LOCUS       DATBSZ010000001         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_888015, whole genome shotgun sequence.
ACCESSION   DATBSZ010000001 DATBSZ010000000
VERSION     DATBSZ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888015"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VIG70_00005"
     CDS             complement(<1..377)
                     /locus_tag="VIG70_00005"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8248961.1"
                     /translation="MRIFLFSLAALAFPVPAQDTAPDALVKSVTLEVIDLISKDKDIK
                     SGSRAKLVEVIEAKVLPHFNFTAMTALAMGQNWNKSTPEQKKRLTEEFKTLLVRTYAS
                     ALAAYSEQKFDFRPLRAKPTDTDV"
     gene            complement(386..1084)
                     /locus_tag="VIG70_00010"
     CDS             complement(386..1084)
                     /locus_tag="VIG70_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY8248962.1"
                     /translation="MTRVVAGMLLLLANGCATSSGEERDPRDPFEGFNRGIYSFNETV
                     DVYVAQPVARAYVKVLHQEIRSRVGNFFSNVQDIFIGVNNFLQGKFVDGVNDWARFTF
                     NTTFGLLGIHDVASEMGLEKHNEDFGQTFGRWGAGSGPYLILPFLGSSTVRDGAGLAI
                     DWWVAPLTEVRPIALRNTLYGVYFVNTRADLLEAGRILEEAALDKYVFQRDAYLQRRR
                     SLIYDGSPPREKYE"
     gene            complement(1092..1565)
                     /gene="mlaD"
                     /locus_tag="VIG70_00015"
     CDS             complement(1092..1565)
                     /gene="mlaD"
                     /locus_tag="VIG70_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEY8248963.1"
                     /translation="MNRSMIDLWVGIFVAVGFAALLFLALKVGNLATFSTGETYAVQA
                     KFANIGGLKVRGPVKSAGVVVGRVADIRFDNESYEAIVWMTIDAHYQFPRDTTAKILT
                     SGILGEQYVGLEAGGDGVMLKHNDRVRLTQSAVVLENLISQFLFNKAAEGKPQEK"
     gene            complement(1567..>2016)
                     /locus_tag="VIG70_00020"
     CDS             complement(1567..>2016)
                     /locus_tag="VIG70_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8248964.1"
                     /translation="AITAEIGLMKATEQISAMEMMAVDPIARVVAPRFWGGVISMPLL
                     AALFSAAGIFGGYLVGVQLIGVDPGAFWSQMQAAVDLRNDILNGVIKSVVFGVAVTWI
                     SVFEGYDAVPTAEGVSRATTRTVVTSSLAILALDFVLTALMFTGGSH"
BASE COUNT          343 a          660 c          664 g          349 t
ORIGIN      
        1 acgtcggtgt cggtcggctt ggcgcgcagc ggccggaagt cgaacttctg ctcgctgtag
       61 gcggcaagcg cgctcgcgta ggtgcgcacg agaagcgtct tgaactcctc ggtgaggcgc
      121 ttcttctgct ccggggtgct cttgttccag ttctgcccca tggcgagcgc ggtcatcgcg
      181 gtgaagttga aatgcggcag caccttcgcc tcgatcacct cgaccagctt ggcgcggctg
      241 ccggacttga tgtccttgtc cttcgagatc aggtcgatga cctcgagggt cacgctcttc
      301 accagcgcgt cgggcgcggt gtcctgcgcc ggcacgggga aagccagggc agccaggctg
      361 aacagaaaaa ttctcattct gtatttcatt cgtatttctc ccgcggcggg ctgccatcgt
      421 agatcaggct gcgccggcgc tgcagatagg cgtcgcgctg gaacacgtac ttgtcgagcg
      481 ccgcctcctc gaggatgcgg cccgcctcca gcaggtcggc gcgcgtgttg acgaagtaca
      541 cgccgtagag cgtattgcgc aacgcgatcg gccgcacctc ggtcagcggc gccacccacc
      601 agtcgatcgc gaggccggcg ccgtcacgca cggtgctcga gccgaggaac ggcaggatca
      661 ggtacgggcc ggacccggcg ccccagcggc cgaaggtctg gccgaagtcc tcgttgtgct
      721 tctccagtcc catttcggag gccacgtcgt gaatgccgag caggccgaac gtggtgttaa
      781 acgtgaagcg cgcccagtcg ttgacaccat cgacgaactt cccttgcagg aagttgttca
      841 cgccgatgaa gatgtcctgg acgttggaaa agaagttgcc gacgcggctg cggatttcct
      901 ggtgcaggac cttcacgtag gcgcgcgcca cgggctgcgc cacgtacacg tcgacggtct
      961 cgttgaagct gtagatgccg cggttgaagc cctcgaaggg gtcgcgcgga tcgcgctcct
     1021 cgccgctcga ggtcgcgcat ccgttcgcga ggagcagcag catccccgca acaacccgcg
     1081 tcacggtgcc ctcatttttc ttgaggcttg ccctcggccg ccttgttgaa caggaactgg
     1141 ctgatcaggt tctcgagcac caccgccgac tgcgtgaggc gcacgcggtc gttgtgcttg
     1201 agcatcacgc cgtcgccccc ggcctcgagc ccgacgtact gctcgccgag gatccccgag
     1261 gtgaggatct tggcggtggt gtcgcgcgga aactggtagt gggcgtcgat ggtcatccac
     1321 acgatcgcct cgtagctctc gttgtcgaag cggatatcgg cgacgcggcc caccaccacg
     1381 ccggcgctct tgaccggccc gcgcaccttg aggccgccga tgttggcgaa cttggcctgc
     1441 accgcgtagg tctcgccggt ggagaaggtg gcgaggttgc ccaccttcag cgcgaggaac
     1501 aagagcgccg cgaagcccac cgcgacgaag attccgaccc agagatcgat catcgagcgg
     1561 ttcatgtcag tggctgcctc cggtgaacat cagcgcggtc aggacgaaat cgagcgcgag
     1621 gatcgcgagc gacgaggtca ccacggtgcg cgtggtggcg cgcgacacgc cctcggcggt
     1681 gggcaccgcg tcgtagccct cgaacaccga gatccaggtg accgcgacgc cgaacaccac
     1741 gctcttgatg acgccgttga gaatgtcgtt gcgcaggtcg accgcggcct gcatctgcga
     1801 ccagaacgcg ccggggtcga cgccgatcag ctgcacgccg acgaggtagc cgccgaagat
     1861 gccggcggcg gaaaacagcg ccgcgagcag cggcatcgag atcaccccgc cccagaagcg
     1921 cggcgcgacc acgcgcgcga tcgggtcgac cgccatcatc tccatcgccg agatctgctc
     1981 ggtcgccttc atcaggccga tctcggcggt gatcgc
//
LOCUS       DATBSZ010000002        18292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1093120, whole genome shotgun sequence.
ACCESSION   DATBSZ010000002 DATBSZ010000000
VERSION     DATBSZ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18292
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093120"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VIG70_00025"
     CDS             <1..608
                     /locus_tag="VIG70_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEY8248965.1"
                     /translation="SRLLGALGRASGPVLDLEELAAHRGSVLGNLPDRPQPSQKMFES
                     ALLQELSNLPGERPVFVEGESKKIGELQVPEALMARMRGSPCVVLETALEARVDLLLD
                     EYRHFVADRSALDRQLDCLVALHGRGRIAEWKALAARGEWREFVARLLVEHYDPAYRR
                     SSQRNFIHLSDAVKVSVSSADEAAFARAAGALLGASAAVAA"
     gene            611..2374
                     /locus_tag="VIG70_00030"
     CDS             611..2374
                     /locus_tag="VIG70_00030"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8248966.1"
                     /translation="MSRVWVLVAALLAPGLCAAQTASQRLDRLAAESRERELDLFPVG
                     EIFSRGAGPRQDRFEQIFTEEHRARQRAHHRRILSELGGIPVAELGPSEKLTHELLGW
                     RARNSLEWLTHPFHQHSALNHIDGGIAFGLVRVVQTQPFRSEADYQAWFRRLRHYPAF
                     LQDAERLLREGIASGVTTPRVLAERALSQLEGLASEDMAKSALWNPMRQFPASMDADA
                     RKRVEAEYRRLLADEMVPALRRLAAFVRNDYLAKARTSDGFGALPNGAAMYRYAVRVN
                     TTTELTPDEIHELGLREVKRIQPSFLAAAKKAGYDGAIKDFRAWLRDNPRNFPFTSGD
                     EVIAHLNAIHARIVPQLPKLFGRMPKARFEIRLTDPAIAAGTPAQYYWPTDDGRPGIF
                     AMPVVNARDTSIFGLTALLVHEGMPGHHFESLKLELKAPEFRRRLSITAFSEGWALYA
                     ESLGHELGVYDEALALAGRYSFELWRAGRLVLDTGLHARGWTREQAIRYFIEECGMTE
                     DAATNEVLRYMVWPGQALSYKIGELTILDLRAKAEKRLGKRFDLRAFHDAILEEGHLP
                     LSMLKSRMEPWIDAQDKKRNL"
     gene            2346..2717
                     /locus_tag="VIG70_00035"
     CDS             2346..2717
                     /locus_tag="VIG70_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8248967.1"
                     /translation="MRRTRNAICSLALALACGAVAAATLFHHIHNAEFLHDYPNLPAW
                     LSRAGVYAAWLIATAIGVIGYRLRHRGLLVLYACYALDGLVHYALAPMSAHTPMMNTT
                     IWLEAAAGAALLIMVFRRRTE"
     gene            2737..3063
                     /locus_tag="VIG70_00040"
     CDS             2737..3063
                     /locus_tag="VIG70_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8248968.1"
                     /translation="MRARLYPLALWLLPGTAFGSDPTGLFVLLFGMPAVAAAVVSLPI
                     SWWAPKLGAAVTGLLLALHMPLVWWANDVGYLKSAGGWIYLSLSLTAIALMISIARLS
                     RGRPSP"
     gene            complement(3038..3967)
                     /locus_tag="VIG70_00045"
     CDS             complement(3038..3967)
                     /locus_tag="VIG70_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY8248969.1"
                     /translation="MTTTAFITHADCLKHDMGAHHPERPARLTAIEDQLIASGVGQHL
                     TRFEAPLATDEQLARVHPPDYVRAIRDAAPRSGTVHLDPDTAMNPHTLQAALRAAGAA
                     VLAVDLVLQRKAGSAFCAVRPPGHHACRARPMGFCIFNNVAVAARHALEAHGLERVAV
                     IDFDVHHGNGTEDIFEGDEHVLMASTFQHPFYPYSGTDNPASNMVNVPLAAGAGSKEF
                     REAVRAEWIPALDDFAPQLIVFSAGFDAHAEDDMAMLRFTDADYGWVTEQLKSVAERH
                     SKGRMVSMLEGGYALSALGRSAVQHIKVMAGLG"
     gene            4024..4971
                     /locus_tag="VIG70_00050"
     CDS             4024..4971
                     /locus_tag="VIG70_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8248970.1"
                     /translation="MLLKAEFPQPSVASSPIDRVVDAASEIILGKETQVRLALACLLA
                     RGHLLIEDLPGVGKTTLAHVLARTLGLHFQRIQFTSDMLPADILGVSIYERDSGNFKF
                     HPGPIFAQVILADEVNRSTPKTQSALLEAMEEHQVTAEGETRKLPAPFFVIATQNPSE
                     QVGTFPLPESQLDRFMMRIELGYPDRDAERALLSGTDRRDLLATLDPCMTPAELMELQ
                     SGVQRIHVAPALLDYVQAIVEYTRRSPEYVTGLSPRAALALVHSAKAWALLEGRDKVI
                     PEDVQAIVPGVASHRLRPAHDGTRRVDVGAQLLAAVPIP"
     gene            5039..7600
                     /gene="glgP"
                     /locus_tag="VIG70_00055"
     CDS             5039..7600
                     /gene="glgP"
                     /locus_tag="VIG70_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY8248971.1"
                     /translation="MQPGPPHPLSTPTHYSLEVNPRIPKRLARLQELANNLWYSWDRP
                     TRSLFAKLNPALWDAVGHNPKAMLKRIDEQRLLDAAADPAFLDSLNRVLAAFDAYHAE
                     PPFKQNSGGFRPEDLVAYFCAEFGVHESLPLYSGGLGILAGDHCKAASDFQLPFIAMG
                     LLYRQGYFIQTIDGEGRQRADYHDSDFDDLPIAPARRDDGSDLRVEVPFPGRKVIAKV
                     WQARVGHVTLYFLDTDLPENSERDQNIAHRLYGGDRVTRLEQEFVLGVGGARALAAMG
                     HKPTVWHINEGHAAFLVLERVRGLMHEGLDLDAAVEAVASNTVFTTHTPVPAGHDHFP
                     ETAVLPYLKAAFGEDHLAALGRAPAGGDFNMTALALRGSRYQNGVSRIHGGVSARLLS
                     DFWPQVSPEENPIGYVTNGVHVATFLAPEWSELLDRFLGVGWMHRLGHPGIWEKIRGI
                     PDHMFWSVRQDIKARLFHMIRHRMALQHTRNHGSESHLERLLRYADPVNPNVLTIGFG
                     RRFATYKRATLLFNDLDNVRRLLSDRTRQVLFLFAGKAHPADEPGQELIRTLAHMSHQ
                     GEFTGRILFIEGYDLHIARRLVSGVDVWLNNPVHPLEASGTSGMKAGMNGVVNLSILD
                     GWWDEGYDGENGWAIKPASPQLDPHRRDSEEARTLYEILQDRVIPLYYDRTEGGYSPG
                     WIRMAKRAMASILPRYDASRMLGEYVSKYYIPASRQGHRYAQNGYEAAKTVSAWKARV
                     RAAWSGVAARRLDTPKTRIQFGESLPVEVAVKLNGLAPGDVFVELLLARGAREGTPVQ
                     HTHELSDAGPLSDGEHRFRTELKPGLAGRLDYRIRIFPRNELLTHPFELGLQCWV"
     gene            complement(7560..9518)
                     /gene="glgX"
                     /locus_tag="VIG70_00060"
     CDS             complement(7560..9518)
                     /gene="glgX"
                     /locus_tag="VIG70_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY8248972.1"
                     /translation="MGATWDGAGVNFALFSKNADRVELCLFDPKGRREIERVPVRERS
                     DFVWHCYLPEARPGLLYGYRLHGPHEPDRGHRFDHNKLLLDPYAKMVKRGRGQVVDPA
                     FTWGDDRPPRTPWQDTVIYELHVKGFTQLHPEVPQQLRGTYAGLATDPVITHLKNLGV
                     TAVELLPIHAIFDERRLLHHGLRNYWGYNSIGFFAPEMRYSASGTLGEFKTMVKTLHA
                     AGIEVILDVVYNHTAEGDHTGPTLSFRGIDNAIYYRLDPAHPRRYLNTTGTGNSFNAA
                     HRVVLALIMDSLRYWVEEMHVDGFRFDLATTLARNTQADYDRNVAFLAAVRQDPVVSR
                     VKLIAEPWDLGEGGYQLGNFPPGWAEWNDKYREAARSYWRGDDGTIGELASRIAGSKD
                     LFGRSGRAPTASINFVTTHDGFTLEDLVSYERKRNEANHEGNRDGTDDNRSWNCGVEG
                     PTEDRSVLEIRKKQKRNLLATLLFSHGVPMLVAGDELGRTQGGNNNAYCQDNEVSWVD
                     WEAADRDLLAFTAKLLTLRKTHPPFRRRTYPKPEDTGWLSPEGREMTDQDWNLPFARC
                     LGCLMDGQRLAERDERGAPIVDDDLLLLLNAHHEAVEFSLPEDSWRVLLDTARPQPTL
                     AGNSYALEARSVVLLARPSTAGRARTDA"
     gene            complement(9591..10019)
                     /locus_tag="VIG70_00065"
     CDS             complement(9591..10019)
                     /locus_tag="VIG70_00065"
                     /inference="COORDINATES: protein motif:HMM:NF027482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HEY8248973.1"
                     /translation="MLRAWLVALALVLPFPAAAAEGQVTGADVAEIRAVIHRQIDAFR
                     RDDARSAFALVSPGVQRTFGTPERFLDAVRMGYRAVYRPASVSFLQLVVMSGDVVQQV
                     QVTDRSGSVWVAYYAMQRQRDGSWRTNGCHLIQPSRIISA"
     gene            complement(10020..10670)
                     /locus_tag="VIG70_00070"
     CDS             complement(10020..10670)
                     /locus_tag="VIG70_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY8248974.1"
                     /translation="MAVPNPNKILTFAILSAGALLIAAAPPDAVAPDAPIVSCAPAEG
                     DDLSAATAPEPLDATQQALAEHVSRRFFVAAAATERMVAAAYRAALEVGLDPLLVLAV
                     ISIESRFNPIAESGMGAKGLMQIIPKYHLDKLRAAGGEDAVFDPESNIHVGARILQEY
                     VHRTGTLEAGLQFYNGAWRDNSAQYAHKVMSERSRLEQVLRDRKLPLKVAANARGI"
     gene            10887..11864
                     /locus_tag="VIG70_00075"
     CDS             10887..11864
                     /locus_tag="VIG70_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY8248975.1"
                     /translation="MNIVAMVLAGGEGTRLYPLTAEHAKPAVPFAGGYRLVDFVLSNL
                     VNSRISTIYVLAQYKPESLIKHVADAWSPWFADGDGSVKVLLPRSNTLGGQFKGTADA
                     VYQCLDLLQAHSPDLVAVFAADHVYRMDVRQMVAFHRESRADVTVSAVAVPLKEASSF
                     GILATNPEGRVTQFREKPKDAAPLPSDPSRAYASMGNYLFDPDVLEQALHEARRAGET
                     DFGRDILPRLCGSRRVYAYDFAENRVPGLQEHEERGYWRDVGTLAALAAAQQDAMGSQ
                     PRFNLWNRRWPIRGEFDAALLTRIRSVTAGVPWQAASRAERTGERLPAS"
     gene            complement(11794..13158)
                     /gene="glgA"
                     /locus_tag="VIG70_00080"
     CDS             complement(11794..13158)
                     /gene="glgA"
                     /locus_tag="VIG70_00080"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEY8248976.1"
                     /translation="MRVLFVTPECTPLAKAGGLGDVSAALPPALRALGVQVDVLLPGY
                     AQVLRQIGDATEVARFSELGLDCRVLKNNPLLVLDCAPLYQREGIYQDPGGQDWPDNA
                     LRFGLLSKVAARLADGYDVVHCNDWPAALMPVYSQRPSLLTVHNLAFQGNFERSWLAR
                     LGLPEPSFSMHQLEFHGRISFLKGGLVNAGAINTVSPTYAREIQTGEFGCGLDGLLRE
                     RHAVLSGILNGIDTDAWNPASDPHLAQPFDASSLDRKASNKRALQRRLNLEPSDELLI
                     GLVGRLTHQKGIDLVAAAAHELAALPAQLAVLGQGERSLEGALAAAAARHPGRIALAT
                     GFDEELAHLIEGGADIFLMPSRFEPCGLNQMYSQRYGTPPVARATGGLVDTIVDGETG
                     FLFDRAEPAALAAAVRRSIAVWREPARWREVQRAGMGRDFSWRAAARQYAQLYSRLAT
                     ERPR"
     gene            complement(13270..13536)
                     /locus_tag="VIG70_00085"
     CDS             complement(13270..13536)
                     /locus_tag="VIG70_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8248977.1"
                     /translation="MKPDVAEKLGEARDFYALTRAVLSMCEPYGPVHAFRLTHNRGAA
                     RVACLIELESPKLQPALARALGAKVLNGAACLEIPVNRDFKTTS"
     gene            complement(13609..14823)
                     /locus_tag="VIG70_00090"
     CDS             complement(13609..14823)
                     /locus_tag="VIG70_00090"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEY8248978.1"
                     /translation="MSDSIHALTGMAGLLALAWLASENRRAIPWRAVTAGLALLVLLA
                     VIFLKIPLVKDAFLKLNDALLVLERATQAGTSLVFGYLGGGSTPFAVSDPASNFVLAF
                     RALPLVLVISALSALLFYWRALPAIVRAISFLLEKAMRVGGVVGLSTAANVFVGMVEA
                     PLFVRPYLARVSRGELFAIMAGGMASIAGTVLFLYGSILGRTQPDAVAHLLIASILSA
                     PAALVIAFLMVPPETTTGGDLVLKSEASGAMDALTRGTLEGAQLLLNIVAMLVVFVAL
                     VALVNLVIAPYTLQGALGWVLAPLAWLAGIPWSEARAAGSLLGTKTVINELVAFLDLA
                     KMTELSERSRLLLTYALCGFANFGSLGIMIGGLGTMSPERRGEVVDLGIKSIAAGTLA
                     TCLTAATVALIL"
     gene            14826..15104
                     /locus_tag="VIG70_00095"
     CDS             14826..15104
                     /locus_tag="VIG70_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY8248979.1"
                     /translation="MESNREFLVACLCAAWCDTCVAYRAGFLAMAERFPRAEFRWVDI
                     EDDAEAVEDMDVDNFPTILVQRGSETLFNGVMLPHHEHLERLLQKIIV"
     gene            complement(15094..15798)
                     /gene="pyrF"
                     /locus_tag="VIG70_00100"
     CDS             complement(15094..15798)
                     /gene="pyrF"
                     /locus_tag="VIG70_00100"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY8248980.1"
                     /translation="MTSIPRAERLIVALDVPSAKEARALAEKLGDAVRFYKIGLELFT
                     AEGYFELLTWLAGRGCKVFADLKLYDIPETVRRAVANLRARGATFLTVHGHRSVMEAA
                     AREKGAMKILAVTVLTSFDHRDLQEMGSTRRVEELVLARARGAAESGCDGVIASGLEA
                     KALKAEFKDRLLVVTPGIRPVGNPTDDQKRTVDVAQAFANGADYIVIGRPIRDAKDPR
                     AAAEAIQGTIASHFKQ"
     gene            complement(15795..16961)
                     /locus_tag="VIG70_00105"
     CDS             complement(15795..16961)
                     /locus_tag="VIG70_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEY8248981.1"
                     /translation="MEKNTWRGFADFCLAVFWRFYEERALQTAGSLTYTTLLSLVPLF
                     TVALAVSTAFPVFDQTMGALQEFVLENFLPDARGIDTIADQITSFTKNAGRLTAIGMG
                     VFIVTAVMLMTTIDISLNRLFRVQRVRPLLQQVLMYWAVLTLGPVLIGGSLSMTSFAV
                     GASFGWLRLSIVADIVLGILPFLFTCIALTLLYVLVPNRAVKPRDALIGGILAGVAFE
                     FAKRGFAIYLARFPTYTLIYGAFATIPIFLVWLYMSWVVVLAGATLTAMLPAYRFAEG
                     KPIPGRDFMDALAVLSVLARAQEKGGPVRLAQISNQVRLMPHRCEAALERAARLGWAV
                     RTDKDSWVLARDADAVRVADLYRSFAFDAETWGIGDADLKRSLRDFATKDKEKK"
     gene            16982..17572
                     /gene="wrbA"
                     /locus_tag="VIG70_00110"
     CDS             16982..17572
                     /gene="wrbA"
                     /locus_tag="VIG70_00110"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEY8248982.1"
                     /translation="MAEILVLYYSVGGSVKRMAELVASGIERVPGATARVRTVPRVST
                     GIEPQPAVPDAGPPYAELRDLEECAGLALGSPTRFGNMAAPLKHFLDGTALLWQRGTL
                     AGKPACVFTSTASLHGGQETTLVSMMLPLLHHGMLVMGLPYTLAELNATRTGGTPYGA
                     SHFAGVTDDQPITDAERSLCIAQGKRLAEIALKLGR"
     gene            17617..17826
                     /locus_tag="VIG70_00115"
     CDS             17617..17826
                     /locus_tag="VIG70_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEY8248983.1"
                     /translation="MSTIDIAKLSPEERLELLERLWDSLSPEAIPMTDAQKEELDRRL
                     DELDREGPVGIPWNEVLDRIRNRAL"
     gene            18072..>18292
                     /locus_tag="VIG70_00120"
     CDS             18072..>18292
                     /locus_tag="VIG70_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8248984.1"
                     /translation="MAEVESVIGDKIKKEITEIEHRTDRCHARRNSVLQWIPRAMTNA
                     RVLFCASLILALFGPLMAQAQPAKTWRLG"
BASE COUNT         2999 a         6217 c         6130 g         2946 t
ORIGIN      
        1 agagccgcct cctcggagcg ctcgggcgcg ccagtgggcc ggtgctcgac ctcgaggagc
       61 tcgccgcgca ccgcggctcg gtgctcggca acctgccgga tcgcccgcag ccctcgcaga
      121 aaatgttcga aagcgcgctg ctccaggagc tctcgaatct tccgggggaa aggccggttt
      181 tcgtcgaagg cgaatcgaag aagatcggcg agctccaggt gcccgaagcg ctgatggcgc
      241 gcatgcgcgg ctcgccttgc gttgtcctgg aaacggcgct cgaggcccgc gtcgacctgc
      301 tgctcgacga gtatcgtcac ttcgtcgccg accggagcgc gctcgaccgg cagctcgact
      361 gcctcgtcgc gctgcacggc cgcggcagga tcgccgagtg gaaggcgctc gccgcgcgcg
      421 gcgaatggcg cgagttcgtc gcgcgcctgc tggtcgagca ttacgatccc gcgtaccgcc
      481 gctcgtcgca gcgcaacttc atccatctct ccgacgccgt gaaggtgtcc gtctccagcg
      541 ctgacgaagc ggctttcgcg cgcgcggcgg gcgctttgct gggcgcaagc gctgccgtag
      601 ccgcgtaaga atgagtcgcg tttgggtcct ggtcgctgcg ctgctggcgc ctggactttg
      661 cgcagcgcag acggcgtcgc agcggctcga ccggctcgcc gccgaatcgc gcgagcgcga
      721 gctggatctg tttcccgtcg gcgagatttt cagccgcggc gccgggccgc ggcaggaccg
      781 cttcgagcag atcttcaccg aggagcatcg cgcgcgccag cgcgcgcatc accgccggat
      841 cctgagcgag ctcggcggga ttcccgttgc cgagctgggg ccgagcgaga agctcacgca
      901 cgagttgctc gggtggcgcg cgcgcaactc gctcgagtgg ctgacgcatc cgttccacca
      961 gcattcggcg ctcaatcaca tcgacggcgg gattgccttc ggcctggtga gggtggtgca
     1021 gacgcaaccg ttcaggagcg aagccgacta tcaggcctgg ttcaggcgcc tgcgccacta
     1081 tcccgcgttt ttgcaagatg ccgagcggct gctgcgcgag ggcatcgcga gcggcgtgac
     1141 gacgccgcgc gtgctcgcgg agcgagcgct gtcgcagctt gaagggctcg cgagcgagga
     1201 catggcgaag agcgcgctgt ggaaccccat gcggcaattc ccggcgtcga tggacgccga
     1261 cgcgcgtaag cgggtcgagg cggagtaccg ccggctgctc gccgacgaga tggttcccgc
     1321 gcttcgccgc ttggcggcgt tcgtacgcaa cgattacctg gcgaaagcgc gcacctcgga
     1381 cgggttcggc gcgctgccga acggcgcggc gatgtaccgg tacgcggtgc gcgtcaatac
     1441 caccacggag ctcacgcccg acgagatcca cgagctcggc ctgcgggagg tgaagcgcat
     1501 ccagccgagc ttcctcgccg cggcgaaaaa ggcaggctac gacggcgcga tcaaggattt
     1561 cagggcttgg ctgcgggata acccgcggaa cttcccgttc acctccggcg acgaggtgat
     1621 cgcgcacctg aacgcgatcc acgcgcgcat cgtgccgcag ctgccgaagc tcttcggccg
     1681 catgccgaag gcgcgctttg agatccggct caccgacccg gcgatcgcgg cgggcacgcc
     1741 ggcgcaatac tactggccga ccgacgacgg ccggcccggg atcttcgcca tgcctgtggt
     1801 caacgcacgc gacacctcga ttttcggcct caccgcgctg ctggttcacg agggcatgcc
     1861 gggccatcac ttcgagagtc tcaagctcga gctcaaggcg ccggaattcc gccggcggct
     1921 gtccatcacc gccttcagcg agggctgggc gctgtacgcc gagtcgctcg gccatgagct
     1981 cggcgtctac gacgaggcgc tggcgctggc cggccgctat tcgttcgagc tgtggcgcgc
     2041 cggccgcctc gtgctggaca ccgggctgca cgcgcgaggc tggacgcgcg agcaggcgat
     2101 ccgctacttc atcgaggaat gcggcatgac cgaggacgcc gcgaccaacg aggtgctgcg
     2161 ctacatggtg tggcccgggc aggcgctcag ttacaagatc ggtgagctca ccatcctgga
     2221 tctgcgcgcg aaggccgaga agcgcctggg caagcgcttc gacctgcgcg ccttccacga
     2281 cgcgatcctc gaggaaggcc atcttccgct cagcatgctg aagagccgca tggagccctg
     2341 gatcgatgcg caggacaaga aacgcaatct gtagcctggc gctcgccctc gcctgtggcg
     2401 cggtcgcggc cgcgaccctg ttccatcaca tccacaacgc ggagttcctg cacgactatc
     2461 cgaatcttcc ggcctggctc tcgcgcgccg gcgtctatgc ggcctggctg attgcgacgg
     2521 cgatcggcgt gatcggctac cgcttgcgtc atcgcggctt gctggttctc tatgcctgtt
     2581 acgcgctgga tggcctcgtc cactacgcgt tggcgccgat gtcggcgcac acgccgatga
     2641 tgaacactac gatctggctc gaagcggcgg ccggcgcggc gctgctcatc atggtttttc
     2701 gacggaggac cgaatgaaca cgcagcgtct cgtagcgtga gagcgcggct ttatccgctc
     2761 gcgctctggt tgcttcccgg gactgccttc ggctcggacc cgacgggact cttcgtgctt
     2821 ctcttcggca tgcctgccgt agcggcggcg gtcgtctctc tgccgatttc ctggtgggca
     2881 ccaaagctcg gcgccgccgt gaccggattg ctccttgccc tgcacatgcc gctggtatgg
     2941 tgggcgaacg acgtcggcta cctgaaaagc gccggcggtt ggatctatct ttcgctctcg
     3001 ctgaccgcga tagccctgat gatttccatc gcaaggctca gccgaggccg gccatcacct
     3061 tgatgtgctg cacggcgctg cggccgagcg ccgagagcgc atagccgcct tcgagcatcg
     3121 agaccatgcg gcccttggag tggcgctcgg ccacgctctt cagctgctcg gtgacccagc
     3181 cgtagtcggc gtcggtaaag cgcagcatcg ccatatcgtc ctcggcgtgc gcgtcgaagc
     3241 cggcggagaa gacaatcagc tgcggcgcga agtcgtccag cgccgggatc cactccgcgc
     3301 gcaccgcctc gcggaattcc ttcgagccgg cgcccgcggc gagcggcacg ttcaccatgt
     3361 tcgatgccgg gttatcggtg ccgctgtagg gatagaacgg gtgctggaag gtcgacgcca
     3421 tcagcacatg ctcgtcgcct tcgaagatgt cctcggtgcc gttgccgtgg tgcacgtcga
     3481 agtcgatgac cgccacgcgc tcgagcccgt gggcttccag cgcatggcgc gcggccaccg
     3541 cgacgttgtt gaagatgcag aaccccatcg gccgcgcgcg gcaggcgtga tggcccggcg
     3601 ggcggaccgc gcagaatgcg ctgccggctt ttctttgaag gactaaatcg acggcgagca
     3661 ccgcggcccc ggcggcgcgc aatgccgcct gcagggtatg cggattcatc gcggtgtcgg
     3721 gatcgaggtg cacggtgccc gagcgcggcg cggcgtcgcg gatcgcgcgc acgtagtcgg
     3781 gcgggtgcac gcgggcgagc tgctcgtcgg tggcgagcgg cgcttcgaag cgcgtcagat
     3841 gctgcccgac gcccgaagcg atgagctggt cctcgatcgc ggtgagccgc gccgggcgct
     3901 cgggatgatg cgcgcccatg tcgtgcttca ggcagtccgc gtgagtgata aaggcggttg
     3961 ttgtcatgca aaggcggaag tagtcacgcg atttcatcat agcgcatcga gtacaatcgt
     4021 cgcatgctcc tgaaggccga atttccgcag ccgagcgtcg cgtcgtcgcc gatcgaccgc
     4081 gtcgtagacg cggcgagcga gatcattctc ggcaaggaga cccaggtgcg cctcgcgctc
     4141 gcctgcctgc tcgcgcgcgg ccacctgctg atcgaggacc tgcccggcgt cggcaagacc
     4201 acgctcgcgc acgtgctcgc ccggacgctc gggctgcact tccagcgcat ccagttcacc
     4261 agcgacatgc tgcccgccga catcctgggc gtgtcgattt acgagcgcga cagcggcaac
     4321 ttcaagttcc accccggccc gatctttgcg caggtgatcc tcgccgacga ggtgaaccgc
     4381 tccacgccga agacgcagtc ggcgctgctc gaagcgatgg aggagcacca ggtgaccgcg
     4441 gagggcgaga cgcgcaagct gccggcgccg ttcttcgtca tcgcgacgca gaacccgtcg
     4501 gagcaggtcg gcacctttcc gctgcccgag tcgcagctcg accgcttcat gatgcgcatc
     4561 gagctcggct accccgaccg cgacgccgag cgcgcgctcc tctccggcac cgaccggcgc
     4621 gacctgctgg cgacgctcga cccgtgcatg acgccggccg agctgatgga gctgcagtcc
     4681 ggtgtgcagc ggatccacgt cgcccccgcg ctgctcgact acgtgcaggc gatcgtcgag
     4741 tacacgcggc gctcgcccga gtacgtgacc gggctctcgc cgcgcgcggc gctcgcgctg
     4801 gtgcactcgg cgaaggcctg ggcgctgctc gaaggccgcg acaaggtgat cccggaggac
     4861 gtgcaggcga tcgtgcccgg cgtcgcctcg caccgcctgc gccccgcgca cgacgggacc
     4921 cggcgcgtcg acgtcggcgc gcagctcctc gcagcggttc cgatcccctg aatggccggg
     4981 gtgtcgcgtt ccggcaacgc atcgcgcgca acgctgattc ggtagaatcc gccgcttcgt
     5041 gcagcccggt cccccgcatc cgctgagtac gcccacccac tactcgctcg aggtcaaccc
     5101 gaggatcccc aagcgcctcg cgcgcctgca ggagcttgcc aacaacctct ggtacagctg
     5161 ggatcggccg acgcgctcgc tgttcgcgaa gctgaatccg gcgctgtggg acgcggtcgg
     5221 gcacaacccc aaggcaatgc tcaagcgcat cgacgagcag cgccttctcg acgcggcggc
     5281 cgatccggca ttcctggaca gcctcaaccg cgtgctcgcg gcgttcgacg cctaccatgc
     5341 agagccgccg ttcaagcaga actcgggcgg cttccggccc gaagacctgg tggcgtactt
     5401 ctgcgccgag ttcggcgtgc acgaaagcct gccgctgtat tcgggcggcc tcggcatcct
     5461 cgccggcgac cattgcaagg cggcgagcga cttccagctg ccgttcatcg ccatgggcct
     5521 gctttaccgg cagggctact tcatccagac gatcgacggc gagggccgcc agcgcgccga
     5581 ttaccacgac tcggacttcg acgacctgcc gatcgctccg gcgcggcgcg acgacggctc
     5641 ggacctgcgg gtcgaagttc ccttccccgg gcgaaaggtc atcgcgaagg tctggcaggc
     5701 gagggtcggc cacgtcacgc tgtatttcct cgacaccgac ctgccggaga acagcgagcg
     5761 cgaccagaac atcgcccacc gcctctacgg cggcgaccgc gtcacgcgcc tggagcagga
     5821 attcgtgctc ggcgtcggcg gcgcgcgcgc gctcgccgcg atgggccaca agcccacggt
     5881 gtggcacatc aacgaggggc atgccgcctt cctcgtcctc gagcgcgtgc gcggcctgat
     5941 gcacgagggg ctcgacctcg acgccgcggt ggaagccgtc gcctcgaaca cggtgttcac
     6001 cacgcatacc ccggtgcccg ccggccacga tcacttcccc gagacggcgg tgttgcccta
     6061 cctgaaggcc gcattcggcg aagaccatct cgccgcgctc ggccgcgcgc cggcgggcgg
     6121 cgacttcaac atgaccgcgc ttgcgctgcg cgggtcgcgc taccagaacg gcgtgtcgcg
     6181 catccacggc ggggtctcgg cgcggctgct gagcgacttc tggccgcagg tatcgccgga
     6241 ggaaaacccg atcggctacg tgacgaacgg cgtgcacgtc gcgaccttcc tcgcgccgga
     6301 atggagcgag ctgctcgacc gcttcctcgg cgtgggctgg atgcaccgtc tcggccatcc
     6361 cggcatctgg gagaagatcc gcggcatccc cgaccacatg ttctggagcg tgcgccagga
     6421 catcaaggcg cgcctgttcc acatgatccg ccaccgcatg gcgctgcagc acacgcgcaa
     6481 ccacggcagc gagtcgcacc tcgagcgcct gctgcgctat gccgacccgg tcaacccgaa
     6541 cgtgctcacg atcggcttcg gccgccgctt cgccacctac aagcgcgcca cgctgctctt
     6601 caacgatctc gacaacgtgc gccgcctgct ctccgaccgc acgcgccagg tgctgttcct
     6661 gttcgccggc aaggcgcacc cggccgacga gcccgggcag gagctcatcc ggacgctcgc
     6721 gcacatgtcg caccaggggg aattcaccgg ccggatcctg ttcatcgaag gctacgacct
     6781 gcacatcgcg cggcgcctgg tgtccggcgt cgacgtgtgg ctgaacaatc cggtgcaccc
     6841 gctggaagcc tcgggcactt ccggcatgaa ggcggggatg aacggcgtgg tcaacctctc
     6901 gatcctggac ggctggtggg atgagggcta cgacggcgag aacggctggg cgatcaagcc
     6961 cgcctcgccg cagctcgatc cgcaccgccg cgacagcgag gaggcgcgca cgctctacga
     7021 gatcctgcag gaccgcgtga tcccgctgta ctacgaccgc accgagggtg gctattcgcc
     7081 gggctggatc cgcatggcca agcgcgcgat ggcatcgatc ctgccgcgtt acgacgcctc
     7141 gcgcatgctc ggcgagtacg tctccaagta ctacatcccg gcctcgcggc agggccaccg
     7201 ctatgcgcag aacggctacg aggcggccaa gaccgtttca gcctggaaag ccagggtgcg
     7261 cgcggcgtgg agcggcgtcg cggcgcgccg cctcgacacg cctaagacgc gcatccagtt
     7321 cggcgagtcg ctgccggtcg aggtcgcggt caagctgaac ggcctcgcgc ccggcgacgt
     7381 gttcgtcgag ctattgctcg cgcgcggtgc gcgcgaaggc acgccggtgc agcacacgca
     7441 tgaattaagc gatgctggcc cgctgtccga cggggagcac cgcttcagaa ccgaactcaa
     7501 gcccggcctc gccggccggc tcgactatcg catccggatc ttcccgcgca acgagctcct
     7561 tacgcatccg ttcgagctcg gcctgcagtg ctgggtctag cgagcagcac caccgagcgc
     7621 gcttccagcg catagctgtt cccggcaagg gtgggctggg gacgcgccgt gtccagaagc
     7681 actctccacg aatcctccgg aaggctgaat tcgaccgctt cgtggtgcgc gttgagaagc
     7741 agcagcaggt cgtcgtccac gatcggcgcg ccgcgctcgt cgcgctcggc gaggcgctgg
     7801 ccgtccatca ggcaccccag gcagcgggcg aacggcaggt tccagtcctg gtcggtcatc
     7861 tcgcgcccct cgggcgagag ccagccggtg tcctcgggct tgggatacgt gcggcgccgg
     7921 aacgggggat gggtttttct cagcgtcaat aatttcgccg tgaaggcgag caggtcccga
     7981 tccgccgctt cccagtcgac ccacgacact tcgttgtcct ggcaataagc gttgttgttg
     8041 ccgccctgcg tgcggccgag ctcgtcgccc gcgacgagca tcggcacgcc gtgcgagaag
     8101 agcagcgtcg cgagcaggtt ccgcttctgt ttttttctta tttcaagaac agaacgatct
     8161 tccgtcgggc cctcaacacc gcaattccag cttcggttgt cgtcggtgcc gtcgcggttg
     8221 ccctcgtggt tggcctcgtt tcgcttgcgc tcgtagctca ccaggtcctc gagcgtgaag
     8281 ccgtcgtgcg tggtgacgaa attgatgctc gcggtggggg cgcggccgct tctcccgaaa
     8341 aggtccttgg atcccgcgat gcgcgaggcg agctcgccga tcgtgccgtc gtcgccgcgc
     8401 cagtaggacc tcgcggcctc ccggtacttg tcgttccatt ccgcccagcc gggcgggaaa
     8461 ttgccgagct ggtagccgcc ttcgccgagg tcccagggct cggcgatcag cttcacccgc
     8521 gagaccaccg ggtcctggcg caccgcggcg aggaacgcga cgttgcggtc gtagtcggcc
     8581 tgcgtattgc gcgcgagcgt ggtggcgagg tcgaagcgga agccgtcgac gtgcatctcc
     8641 tccacccagt agcgcaggct gtccatgatc agcgccagga ccacgcggtg cgcggcgttg
     8701 aagctgttgc cggtgccggt cgtgttgagg tagcgccgcg gatgcgcggg gtcgagccgg
     8761 tagtagatcg cgttgtcgat gccgcggaag ctgagcgtcg gcccggtgtg gtcgccttcg
     8821 gcggtgtggt tgtagaccac gtcgaggatc acctcgatgc cggcggcgtg cagcgtcttc
     8881 accatggtct tgaactcgcc cagcgtaccg ctcgcggagt agcgcatttc cggggcgaag
     8941 aagccgatgc tgttgtagcc ccagtagttg cgcaggccgt ggtgcagcag ccggcgctcg
     9001 tcgaagatgg cgtggatcgg caagagctcg accgcggtca cgcccaggtt tttcaagtgg
     9061 gtgatcacgg ggtcggtggc gaggccggca taggtacccc gaagctgttg cggcacttcg
     9121 gggtgaagct gcgtaaaccc ctttacgtgc agctcgtaga tcaccgtgtc ctgccacggg
     9181 gtgcgcggcg gccggtcgtc cccccacgta aaggccggat cgacgacctg gccgcggccc
     9241 cgcttgacca tcttcgcgta gggatcgagc agcagcttgt tgtggtcgaa gcggtgaccg
     9301 cgatccggct cgtgcggacc gtgcaggcgg tagccgtaca gcaagccggg ccgcgcttca
     9361 ggcaggtagc agtgccagac gaaatcgctg cgctcgcgca ccggcacgcg ctcgatctcg
     9421 cggcggccct tggggtcgaa caggcacagc tcgacgcgat cggcgttctt ggagaacagc
     9481 gcgaagttga ccccggcgcc gtcccaggtc gcgcccaaag gatagggtcg gccgcgccag
     9541 accgcgcgcg gcgctaccag agctcgtgcg ggcgcctcca ctgcgcccga ttatgcagaa
     9601 ataatccggc ttggctggat caggtggcaa ccgttggtcc gccaactccc gtcccgctgg
     9661 cgctgcatgg cgtagtacgc gacccagacc gacccggagc ggtcggtgac ctggacctgc
     9721 tgcaccacgt cgccgctcat cacgacgagc tgcaggaacg acacgctggc ggggcggtag
     9781 accgcgcggt agcccatgcg caccgcgtcc aggaaacgct ccggggttcc gaacgtccgc
     9841 tggacgccgg gagacaccag cgcgaaggcg ctgcgcgcgt cgtcgcggcg gaaagcgtcg
     9901 atctggcggt ggatcaccgc gcggatctcg gcgacgtcgg cgccggtgac ctggccctcc
     9961 gcagccgccg ctggaaacgg caacaccagc gccagcgcga ccagccaggc tctcagcatt
    10021 cagatgcccc gcgcattggc agcgaccttc agcggcagct tgcggtcgcg cagcacctgc
    10081 tcgaggcggc tgcgctcgga catgaccttg tgggcgtact gggcggagtt atcgcgccag
    10141 gcgccgttgt agaactgcag ccccgcctcg agcgtgccgg tgcggtgcac gtattcctgc
    10201 aggatgcgcg cgccgacgtg gatgttgctt tcggggtcga agaccgcgtc ctcgcccccc
    10261 gccgcgcgca gcttgtcgag gtggtacttc gggatgatct gcatcaaccc cttggcgccc
    10321 atgccgcttt cggcaatcgg attgaagcgc gactcgatgg agatgaccgc cagcacgagg
    10381 agcggatcga gcccgacctc gagcgcagcg cggtacgccg cggcgaccat gcgctcggtc
    10441 gccgcggcgg cgacgaaaaa gcgcctcgat acgtgctcgg cgagcgcctg ctgcgtcgca
    10501 tcgagaggct cgggtgcagt cgccgccgaa aggtcatcgc cttcggccgg cgcgcaggac
    10561 acgatcggcg cgtcgggggc gaccgcatca ggcggtgccg ccgcaatcag cagcgctccg
    10621 gcggaaagga tggcgaatgt caggatcttg ttgggattgg ggacggccat gagctcaccc
    10681 agcggtgtgc cgaatggtag ggcgcgggga gaagacatat ctatcgccgc gacttgaatt
    10741 tcctgtcgcg cggcgactac acgcgctccc gcattgacta catcgggata caagtccagc
    10801 cgatagtggc ttccgctacc ggccaatatc atgtcgccgt tactcgacca acaccaactc
    10861 ctgcttacga agaaaggcca acaagcatga atatcgtcgc gatggttctg gcaggcggtg
    10921 aaggcacgcg actgtatccg ctcaccgccg agcacgccaa gccggcagta cctttcgcgg
    10981 gcggctaccg cctggtggac ttcgtgctga gcaacctggt gaattcgcgc atctcgacga
    11041 tctacgtgct cgcgcagtac aagcccgaat cgctgatcaa gcacgtcgcg gatgcgtggt
    11101 ctccgtggtt cgcggacggc gacggctcgg tcaaggtgct gctgccgcgc tccaacaccc
    11161 taggcggcca gttcaagggc accgccgacg ccgtctacca gtgcctggac ctgctccagg
    11221 cgcactcgcc cgacctggtg gcggtgttcg ccgccgacca cgtgtaccgc atggacgtgc
    11281 gccagatggt cgcgttccac cgcgagagcc gcgccgatgt caccgtttcc gccgttgcgg
    11341 tgcccctcaa ggaggcttcg agcttcggca tcctcgcgac caatcccgaa ggccgggtca
    11401 cccagttccg tgagaagccc aaggatgcgg ccccgctgcc ttcagacccc tcccgtgcct
    11461 acgcctcgat gggcaactac ctgttcgatc ccgatgttct ggagcaggcg ctgcacgagg
    11521 cgcgccgcgc cggcgaaacc gacttcgggc gcgacatcct gccccggctg tgcgggtcgc
    11581 gccgcgtgta cgcctacgat ttcgccgaga accgcgtccc gggcctgcag gagcacgaag
    11641 agcgcgggta ctggcgcgac gtcggcacgc tggcggcgct cgccgccgcc cagcaggacg
    11701 cgatgggcag ccagccgcgc ttcaatctct ggaaccgccg ctggccgatc cgcggcgagt
    11761 tcgacgccgc cctgcttacc cggatccggt ccgtcaccgc gggcgttccg tggcaagccg
    11821 cgagtagagc tgagcgtact ggcgagcggc tgcccgccag ctgaagtcgc gccccatccc
    11881 cgcgcgctga acctcgcgcc agcgcgcggg ctcgcgccac actgcaattg atcttctgac
    11941 tgcagccgcc agcgccgcgg gttccgcccg gtcaaacaga aatcccgtct cgccgtccac
    12001 gatcgtatcg accagcccgc cggtcgcgcg cgccaccggc ggggtcccgt agcgctggct
    12061 atacatctgg ttcaacccgc agggctcgaa gcgcgagggc atcaggaaaa tgtcggcgcc
    12121 gccctcgatg aggtgtgcga gctcctcgtc gaagccggtc gcgagcgcga tgcgccccgg
    12181 atggcgcgcc gccgccgcgg cgagcgcgcc ctcgaggctg cgctcgccct ggccgagcac
    12241 cgcgagctgc gcgggcagcg cggcgagctc gtgcgccgcg gccgcgacga ggtcgatgcc
    12301 cttctggtgc gtgaggcggc cgaccaggcc gatgaggagc tcgtcgctgg gctcgaggtt
    12361 gagccggcgt tgcagcgcgc gcttgttcga ggccttcctg tccaggctcg aggcatcgaa
    12421 gggctgagcg agatgcggat cgctcgccgg attccaggcg tcggtatcga tgccgttcag
    12481 gattccgctc agcaccgcat ggcgctccct tagaagcccg tcgaggccgc agccgaattc
    12541 cccggtctgg atctcgcgcg cgtaggtggg gctcaccgtg ttgatggcgc cggcgttcac
    12601 cagcccgccc ttgaggaacg agatgcggcc gtggaactcg agctggtgca tggagaacga
    12661 tggctccggc aggccgaggc gcgcgagcca cgagcgctcg aaatttccct gaaacgcaag
    12721 attgtgaacc gtgagcaagc tgggtctttg agaataaacg ggcatcaaag cggccggcca
    12781 gtcgttgcag tggaccacgt cgtacccgtc ggccagccgc gccgcgacct tcgacagcag
    12841 cccgaagcgc agcgcgttgt ccggccagtc ctgcccgccc ggatcctggt agatgccttc
    12901 ccgctggtag agcggcgcgc aatccagcac caaaagcggg ttgtttttca agacacgaca
    12961 atccaggccg agctcgctga atcgggcgac ctcggtcgcg tcgccgatct gccgcagcac
    13021 ctgcgcatat cccggcagca ggacgtcgac ctgcaccccg agcgcgcgca atgccggcgg
    13081 cagcgcggcg ctgacgtcgc cgagcccgcc cgccttcgcg agcggcgtgc attcgggggt
    13141 gacgaacagg acgcgcatga gcgtccgata ttactgaccc tggaaaaaaa agaccccggt
    13201 agggtgccgg ggtcagttgc ctacatacgc acgaagggag ggaggtgcgt atgcaagggg
    13261 aataagggtc tagctggtcg tcttgaagtc cctgttcacc ggaatctcca gacaagccgc
    13321 gccgttcagc accttggcgc cgagcgcgcg cgccagggcc ggctgcagct tcggcgactc
    13381 gagctcgatc aggcacgcga cgcgcgccgc gccgcggttg tgggtcaggc ggaatgcgtg
    13441 caccgggccg tagggctcgc acatcgacag cacggccctc gtcagcgcgt agaaatcgcg
    13501 cgcttccccc agcttttcgg cgacgtcggg cttcattccg aacgatgcta ttgcccgccc
    13561 cgggggggcg gctatcccaa aatgcgtgaa cctgctgtag tgccgcaact acaggatcag
    13621 tgccaccgtc gccgcggtaa gacaggtggc cagggtgccg gcggcgatcg actttatccc
    13681 caggtccacg acttcgcctc gccgctccgg cgacatggtg ccgaggccgc cgatcatgat
    13741 cccgaggctg ccgaaattgg cgaagccgca cagcgcataa gtgagcagaa gccggctgcg
    13801 ttcggaaagc tccgtcatct ttgccagatc gagaaaggcg acgagctcgt tgatcacggt
    13861 cttcgtcccg agcagcgacc cggccgcgcg cgcttcgctc catgggatgc cggcgagcca
    13921 ggcgagcggc gcgagcaccc agccgagcgc gccctgcagt gtgtaggggg cgatgaccag
    13981 gttgacgagc gcgaccagcg ccacgaacac caccagcatc gccacgatgt tgagcaggag
    14041 ctgcgcgcct tcgagcgtgc cgcgcgtgag cgcgtccatc gctccggagg cttcggattt
    14101 cagcaccagg tccccgcccg tcgtggtctc gggaggaacc atcaggaacg caatcaccag
    14161 cgccgcgggc gccgagagga tcgaggcgat cagaaggtgc gccacggcgt cgggctgcgt
    14221 gcggcccagg atgctgccgt acaggaacag caccgtgccc gcgatcgatg ccatgccgcc
    14281 cgccatgatg gcgaacagct cacccctcga gacgcgcgcg agatacggcc gcacgaagag
    14341 cggcgcctcg accatgccga cgaacacgtt ggccgcggtc gacaggccga ccacgccgcc
    14401 gacgcgcatc gccttctcga gcaggaagga gatcgcgcgc acgatcgccg gcagcgcccg
    14461 ccaatagaag agcagcgccg agagcgcgct gatcaccagg accagcggca gcgcgcggaa
    14521 ggccaggacg aaattcgacg cgggatccga aacggcgaac ggtgtgctcc ctcctcccag
    14581 gtagccgaac acgaggcttg tgcccgcctg cgtggcgcgc tcgagaacca gcagcgcatc
    14641 gttcagctta aggaacgcgt ctttcactag aggtatcttc aggaagatca ccgcgaggag
    14701 gacgagaagc gcaaggccgg ctgtgacggc ccgccacggg atcgcgcgcc ggttctcgct
    14761 ggcgagccag gcgagggcga gcagccctgc cattccggtc agggcgtgaa tagaatcgga
    14821 cacgcgtgga gtctaatcgc gaatttctgg tcgcctgcct gtgcgccgcc tggtgcgaca
    14881 cctgcgtcgc ctaccgcgcc ggcttcctgg cgatggccga gcgcttcccg cgcgccgagt
    14941 tccgctgggt cgacatcgag gacgacgccg aggcggtgga ggacatggac gtcgacaact
    15001 tcccgaccat cctggtgcag cgcggcagcg agacgctgtt caacggcgtg atgctgccgc
    15061 atcacgagca cctggagcgc ttgcttcaaa aaatcattgt ttgaagtggg aagcgatagt
    15121 cccctgaatc gcctccgcgg ccgcgcgcgg atccttcgca tcccggatcg gccgcccgat
    15181 cacgatgtag tcggcaccgt tggcgaacgc ctgcgccacg tcgactgtgc gcttctgatc
    15241 atccgtgggg tttcccacgg gtcgaattcc gggcgtgacg acgagcaacc ggtccttgaa
    15301 ctcggccttc agcgccttcg cctcgaggcc cgaggcgatc acgccgtcgc agccgctctc
    15361 ggccgcgccg cgcgcccgcg ccagcaccag ctcttcgacc ctcctggtcg agcccatttc
    15421 ctggagatcg cgatggtcga agctggtcag caccgtcact gccaggatct tcatcgcgcc
    15481 cttctcgcgc gcggccgcct ccattaccga gcggtggccg tgcacggtga ggaaggttgc
    15541 gccgcgcgcg cgcaggttgg ccaccgcgcg gcgcacggtc tccgggatgt cgtagagctt
    15601 gaggtcggcg aataccttgc agccgcgccc ggcgagccac gtgagaagct cgaaatagcc
    15661 ttcggcggtg aagagctcca gcccgatctt gtagaagcgc accgcgtcgc cgagcttctc
    15721 ggcgagggcg cgcgcctcct tcgcgctcgg cacgtcgagc gcgacgatca ggcgctcggc
    15781 acgggggatg gaggtcattt cttctcctta tccttcgtgg cgaagtcgcg caggctgcgc
    15841 ttcagatcgg catctccgat cccccaggtc tcggcatcga atgcaaacga gcggtagaga
    15901 tcggccactc gaacggcgtc cgcgtcgcgc gccagcaccc agctgtcctt gtcggtgcgc
    15961 accgcccagc cgagccgcgc ggcgcgctcc agcgccgcct cgcagcggtg cggcatcagg
    16021 cgcacctggt tgctgatctg ggcaaggcgc accgggccgc ccttttcctg cgcgcgcgcc
    16081 agcaccgaca gcacggcgag cgcgtccatg aaatcgcgcc ccggaatcgg cttgccttcc
    16141 gcgaagcggt acgccggcag catcgcggtc agcgtggcgc cggcgagcac caccacccag
    16201 gacatgtaga gccagacgag gaaaatcggg atcgtcgcga acgcgccgta gatcagcgtg
    16261 taggtcggaa aacgcgccag gtagatcgcg aagccgcgct tcgcgaactc gaatgcgacg
    16321 ccggcgagga tgccgccgat cagcgcgtcg cgcggcttga ccgcccggtt cggcacgagg
    16381 acgtagagca gcgtgagcgc gatgcaggta aacaagaacg gcaggatccc gagcacgatg
    16441 tccgcgacga tgctcagcct gagccagccg aaggacgcgc ccaccgcgaa cgaggtcatc
    16501 gacaggctgc cgccgatcag caccgggccg agcgtgagca ccgcccagta catcagtacc
    16561 tgctgcagta gcggccgcac ccgctgcacg cgaaacaggc ggttgagcga gatatcgatc
    16621 gtcgtcatca gcatcaccgc cgtgacgatg aacaccccca tgccgatcgc cgtcaggcgc
    16681 ccggcgttct tggtgaacga ggtgatctga tcggcgatcg tgtcgatgcc gcgcgcgtcg
    16741 ggcaggaagt tctcgagcac gaactcctgc agggcgccca tggtctggtc gaacaccggg
    16801 aaggcggtcg acaccgcgag cgccacggtg aagagcggca cgagggagag gagcgtggtg
    16861 taggtcaggc tgcccgcggt ctgcagggcg cgctcctcgt agaagcgcca gaacacagcg
    16921 aggcagaagt cggcgaagcc gcgccaggtg ttcttctcca ttatcctttt atgataccgc
    16981 cgtggccgag attctcgttc tctactacag cgtcggcggc tcggtgaagc gcatggccga
    17041 gctcgtcgcc tccggcatcg agcgcgtgcc cggcgccacg gcccgcgtgc ggaccgttcc
    17101 cagggtttcc accggaatcg aaccccagcc tgccgttccg gatgccggac ctccctatgc
    17161 ggaattgcgc gacctcgagg aatgcgccgg cctcgcgctc ggcagcccga cgcgcttcgg
    17221 caacatggcg gcgccgctca agcactttct cgatggcacc gcgctcctct ggcagcgcgg
    17281 cacgctcgcc ggcaaaccgg cgtgcgtctt cacctccacc gcgagcctgc acggcggcca
    17341 ggagaccacg ctcgtctcga tgatgctgcc gctcctgcat cacggcatgc tggtgatggg
    17401 cctgccctac acgctcgccg agctcaacgc cacgcgcacc ggcggcacgc cctacggcgc
    17461 gagccatttc gccggcgtca ccgacgacca gccgatcacc gacgccgagc gctcgctgtg
    17521 catcgcgcaa ggcaagcggc tcgccgagat tgcgctcaag ctcggcaggt aattcagtgc
    17581 gcaagtgtgc cgcggccggc ggcgtatact gagtagatga gcacgattga tatcgcgaaa
    17641 ttgagccctg aagagcggtt ggagttgctg gaacggctct gggacagcct atctcctgag
    17701 gccatcccga tgactgacgc tcagaaagag gagcttgatc gacggctcga cgagcttgat
    17761 cgcgaaggcc cggtcggcat tccctggaat gaagttctcg accgcatccg caatcgcgcg
    17821 ctctgatgca gttagttgtc cgcccggctg cggcggcgga cattgatgaa gcatttcttt
    17881 ggtacgaaga tcaacgcccg ggcttgggcc aagagtttct agcggcggca caggcagtga
    17941 tcgatgccat tgccaagcac ttccgggttt acgaggaggt tgtcgtggtg gtcgcatgca
    18001 tgcatggtcg ccgcagtccg aggcgatggc aggtgcgcac gtgatggcta acaacgcggc
    18061 ggagtacagg tttggctgag gtcgagtccg tcatcggcga caagatcaaa aaggaaatta
    18121 ccgagatcga gcaccgcacg gatcggtgcc acgcaagacg caacagcgtg ttgcagtgga
    18181 taccaagagc gatgaccaat gcgcgcgtcc ttttttgcgc cagcctgatc ctcgcgctgt
    18241 tcggaccgct catggcgcag gcgcagcctg caaagacctg gcgcctcggc at
//
LOCUS       DATBSZ010000003        17071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_478469, whole genome shotgun sequence.
ACCESSION   DATBSZ010000003 DATBSZ010000000
VERSION     DATBSZ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17071
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478469"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VIG70_00125"
     CDS             <1..189
                     /locus_tag="VIG70_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEY8248985.1"
                     /translation="DGKLEVTATGNADTPMAHGHKCLLTVDVWEHAYYVDYRNERERY
                     VSAILERLNWEFAEKNLG"
     gene            253..471
                     /locus_tag="VIG70_00130"
     CDS             253..471
                     /locus_tag="VIG70_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8248986.1"
                     /translation="MRGNGNEEQQDFAAKLALIESEAYLAAESLPPSTVRDRIQHIAV
                     VAQLLKARLNVTSLTILPPSQQPRRSKT"
     gene            complement(491..3787)
                     /locus_tag="VIG70_00135"
     CDS             complement(491..3787)
                     /locus_tag="VIG70_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY8248987.1"
                     /translation="MQDTHAHAKPIDQVVAALQAHLEHGLTGEEARARLQKFGANELT
                     EKPRPGFLALLGDQFNNYLVIILIIAALIALALGEWVDSVAIMCIVVLNAIVGVIQES
                     KAEQALAALKKMAAPNAQALRDNHQVTVPGRELVPGDIVLLEAGNYVPADLRLVSSVN
                     LKVEEASLTGESVPVEKNARAVLDPEIPLGDRKNSAFMGTMVTYGRGRGLVTGTGMNT
                     QIGLIAEMIQSFEAEDTPLQQKLQQLGKVLGTACIAICAVVLVYGLFRDTRLTEIFSL
                     GLVEYLRAEQKDIINLFMTAVSLAIAAVPEGLPAIVTICLALGMQQMIKRNALIRKLP
                     AVETLGCATVICSDKTGTLTQNEMTVVQGWSGGKRFKVTGEGYDPSGHFLLEDKRFEA
                     AADPDAKLLLQGALLCNDAVLDQGRIIGDPTEGAMAVVAVKAGLQRAELEKSMPRVQE
                     IPFDSERKRMTTIHAVEGKPFAFVKGAPDVVLDLCTHVMKNGQAVPITDPLRKEILDQ
                     NRDLASHALRVLSVAVRPLDAVPENPQPGTVEKDLTFIGLLGMIDPARPEVIEAVRVA
                     NGAGLKSVMVTGDHKDTAEAIAREIGILTPGGLVLTGPEIERMSDAELAASTDKLQVC
                     CRVSPQHKTRIVDAMKASGHVAAMTGDGVNDAPALKRANIGVAMGITGTDVSKQTADM
                     VLTDDNFASIVAAIEQGRIIYSNIRKFVYFLLACNVGEILIIFGAMLFGMPIPLRPVH
                     LLWLNLVSDGAPALALGMEKGDKDIMSHPPRPPKEPVINRDMAIGIGVIALVDAIAIL
                     TVFYLGLQRYPGHLEAAQTLAFVTLCVSELVRAFTARSEYHSIFSIGVFSNRWMVWAV
                     GASLLLVLMVVYVPFLQPFFDTVPLTLDDWLMMAPFFFASAIAMELLKFYFRSRARRQ
                     SEQPVFPLEAPGGNAMLKVLVPVDGSRNCEFAVRHVVRQFMNNTAIEIHLLNVQPPLT
                     SYITRFLSRRNVRDYHQDEAEKALRPVRQILDGFGIPYAVHMEIGDRAECITATAHRL
                     HCDHIVLSAARKNSLTRLVESSTTNRVLELTSVPVEIIAGNAVSRWERYGIPAGLGTL
                     IALLFAAVAD"
     gene            4084..5019
                     /locus_tag="VIG70_00140"
     CDS             4084..5019
                     /locus_tag="VIG70_00140"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8248988.1"
                     /translation="MTHTPDIRRYLKHGTLPQLRAFEASVRLGSFTRAAGELHMAQAT
                     ASVQIKKLGETVGLALFEYVGKRIRLTDAGERVYAGCGEVFRALSDMERGLIGLRGAA
                     CGHLQVAVPSSARYFAGRLLDAFLQHNPDVQASLQVRDRIALAEHDLCMLVEPPPGQE
                     VVAQALVPNPLVVLAAADHPLARDRDIPFERLQHEPFVMREPGSPTRMLVLRFFARHG
                     IVPKSRIEFSSDEAVREAICAGLGISILPRYAVGMDPALARLACLDVQGFPLEAHWHF
                     VYPVGRRLSAAARAFMDFSRAEAKSLFRDCLREAN"
     gene            complement(5021..5572)
                     /locus_tag="VIG70_00145"
     CDS             complement(5021..5572)
                     /locus_tag="VIG70_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8248989.1"
                     /translation="MSRIICGMFDRTIDADAALEELKRAGFQRAEIDAFYVSPPGQHA
                     MTPLGGDSSSSEGSSTAGIGAGIGAAVGAVLGAAAGWAVSGDLGLVVIPLGAGLGAYV
                     GSFAGSMARVRGGHRADASVEHPVEPRGGRMIAINVDRAGTERRAIEILRGHSARDVR
                     RAEGEWADGAWRDFDPRAPLAAV"
     gene            complement(5590..6963)
                     /gene="rpoN"
                     /locus_tag="VIG70_00150"
     CDS             complement(5590..6963)
                     /gene="rpoN"
                     /locus_tag="VIG70_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY8248990.1"
                     /translation="MAPAIALRLRQHLTLTPQVQQALRLLQMSALEFAQEMEQAITAN
                     PFLEENPDAPAVLPGTKSGDGPVGDVMLPQESSGGGAALEREQDEWGGASEAQPSLQE
                     HLREQLRMSRVGDRDRALAEMVIDSLDDDGYFKASFEELAALLPKEHDVLPEEFAAAL
                     KLVQSLDPAGVGARSLDECLRLQLENLPAETPSRAAALAMTNHLSLLGNREWARLQHA
                     IDCSEQELHAARTLIRSLDPRPGHRFGANEARYVIPDVIVRKNRDRWVALINPASLPR
                     LRLNRSYADAIGGRNGANQSLARHLQEARWLLRSIEQRFATIQRVADAIVARQRGFFE
                     YGEVAMKPLTLKLIAGELGLHESTVCRVTNSKYMATPRGLFEFKHFFSRRLATENGGA
                     ASATAVRAVMKELIAAEDPRSPLSDAELARLLAQQGLRVARRTITKYRALMRMPSVDV
                     RRADNRA"
     gene            complement(7118..7600)
                     /locus_tag="VIG70_00155"
     CDS             complement(7118..7600)
                     /locus_tag="VIG70_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEY8248991.1"
                     /translation="MSLDWGAIFGLTVSPLELVVRGTAMYLFLFLIFRVVVRRRIGAV
                     GMADILVLVIIADAAQNGMAGEYRSVTEGAILIGTIIGWSWLIDWLNFKVPALQSWLE
                     APPLLLVDDGRILYRNLRHEFVTEEELKSKLREKGVDDVRQVASAYMEPDGTVSVVRR
                     "
     gene            complement(7724..8005)
                     /locus_tag="VIG70_00160"
     CDS             complement(7724..8005)
                     /locus_tag="VIG70_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8248992.1"
                     /translation="MERLTRRGCKNGTQLAYHPDTMRSILTIAALAGVACGARHFFIF
                     LNEKSKNRLTEKKLEVWEGEGGAVPVAATRTAAQVRPKKPRAASSPGVG"
     gene            8005..9360
                     /locus_tag="VIG70_00165"
     CDS             8005..9360
                     /locus_tag="VIG70_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8248993.1"
                     /translation="MPHLLLVDDDPEALEWLSEFAKAEGFTVSQADSLRAARIHMSRL
                     QPDVLLTDLQLPDGKGVELVADLEQRESTEVVMITGHASVESVVEALRLGASDYLVKP
                     VDTERLKSILRRVPRTGELRAEIGELRDELRKLGRFGHILGSSTPMQKLYDQLGRVAP
                     TSATVLLTGESGTGKELAAQTIHDLSRRKKAPFLPLNCGAVSPQLIESELFGHEKGSF
                     TGADRQHKGYFERAHGGTLFLDEITEMPQELQVKLLRVLETGTFTRVGTTSPISTDVR
                     VLAATNRSPEGAVADGKLREDLYHRLNVFPIGMPPLRERATDIELLAEHFLGILNSQE
                     SATKSFAPATVAALYAHSWPGNVRELKNYVQRAFILADDVIDSNLAPTVVTQQESAPL
                     LSVRVGTTLDDVSRRLIEATLAECGSKRKAADMLGISLKTLYNRLAAYKTETGEERDA
                     A"
     gene            9357..9515
                     /locus_tag="VIG70_00170"
     CDS             9357..9515
                     /locus_tag="VIG70_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8248994.1"
                     /translation="MTPKRRDESRVKSNYLRRRRVAQAATDVTRGLKDTERRGIPNDV
                     PAPRRRRV"
     gene            9571..9831
                     /locus_tag="VIG70_00175"
     CDS             9571..9831
                     /locus_tag="VIG70_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 C-terminal domain-containing protein"
                     /protein_id="HEY8248995.1"
                     /translation="MASTPSSSTQKHIERAGDSAHDVVDRVAETASSYAERFGERADE
                     LLEMKDNWVEGAREYVHEHPVAALGIAVAAGYILSMLMRSRD"
     gene            9833..10225
                     /locus_tag="VIG70_00180"
     CDS             9833..10225
                     /locus_tag="VIG70_00180"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY8248996.1"
                     /translation="MNSLQQPRPRPPGLGELVGRLLGEARSLAADFAELAVLDARRAA
                     IRLSWLLAAVLVVTVLVVTTWMALVAAGVILMLGQGASWIIALAVAAVLNLVGAGALA
                     WWMFALIKELPFTALLRQLRGDAPQKPQ"
     gene            10222..10632
                     /locus_tag="VIG70_00185"
     CDS             10222..10632
                     /locus_tag="VIG70_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8248997.1"
                     /translation="MIPPDSEIFEVEQRIAARRAQVKRHGREAGTRALQSLASPAALI
                     GAAALGFFTATAFARRREKPKHPERRKSDHTKAAKATGLAGVLLPAAMWFVRAQWGSP
                     VRLAQVMLEKFQHRNAPTSLRGDTRRDTRRIAAP"
     gene            10681..13509
                     /locus_tag="VIG70_00190"
     CDS             10681..13509
                     /locus_tag="VIG70_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8248998.1"
                     /translation="MEILSRLTRSAHAAFGLVAALAAMALWGWAVHVPVLRDLGADFA
                     PMSAAAAFAFLLIAGSFLAAERGRQASAYVAAGLAAALAIAALVEHYLALSLNLQIAP
                     AASVTLLVLALVTPLPRGAKALGVPLQGAAAALIGGVAFFALLGLSLRVLRFDIPAPL
                     LGFSAPGALAAMLAAAGLAAARPTVWLLETLAGRRTGAVVTRWLLPAAFIVPLAVGWT
                     RLLAEREGLFGEAVGMALFTTMMIAGFGALILWVARTLDHHERKRAQAEEQAGETREW
                     LQVTLAAIGDGVIATDRAGRVRFLNAAAQRLTGWRAAEAAGRPLDELLQLFDERNGAA
                     LASPLNSCLKTRSISAANGEPALRARDGKVHPVHANAAPIVDSGDGIAGAVLVLREAA
                     AQRQAERAMREAYTELDERVVRRTAALERASTALRERNALLNAITTSTPDLIFAKDRE
                     GRMLMANPAWLTAVGRVREDERPEVTGLDDNERLVLEAGETMILEENVAERTYLATKS
                     PLRDEHGRVIGLIGVATDITERKQAQRDLEKLVVAEQRLRAEAERANRAKDEFLAIVS
                     HELRSPLNALRGWSHLLATTRPLEQPLVERASKAIKRNVDHQTRLIDDLLDTSRIVSG
                     KLTIERRLVNLVEIVLAALDAARPGAASKKIDLRFTPDDASIMVVGDGGRLQQLISNL
                     LSNALKFTPERGAIAVLLLKNAERVQLLVKDNGIGIAPEFLPHVFDRFTQADTSAARR
                     AGGLGIGLALVRHIALLHGGQVRADSPGVGRGSAFTIELPAAPASALRAPAPRPERRG
                     TNEGALGGVRIWILDDDPDAHEVASLTLRQSGALVQSAHSASELAAMLERALPAEGPD
                     VLLVDLAMPGEDGFEALRRVRSLEMASGSERYIPALALTAFTQVEREKLVAAGFADRV
                     DKPIDADKLVAAIRSALHENRRNAMRAA"
     gene            complement(13553..14935)
                     /locus_tag="VIG70_00195"
     CDS             complement(13553..14935)
                     /locus_tag="VIG70_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8248999.1"
                     /translation="MSTRTLEADYLVVGCGAAGMAFTDSLIADSKAQVIMVDRRHAPG
                     GHWNEGYPFLRLHQPSAYYGVNSTPLGGDTIDRHGLNQGLYERASGAEICGYYDRVMQ
                     QRLLPSGQVRYFPMCDYVGEHRFVSRVSGERFEVKVKKKRVDAAYLQPSVPASCPPPF
                     EVAQGARCVPVNDLPRMAPQARGYVIIGAGKTAIDACLWLLEIGVPPGDIRWIQPRES
                     WLANRVFLQGGEQVGKFIEGLSLQLEAAAQATSLDDLFRRLNAAGQLLRVDGSAKPAK
                     YTSATVTAGEITELARIENVVRLGYVRRVERDSILLDQGTVATSPDHLHVHCAARGLN
                     PAPGIPIFANDRITLQPIRPGLVPFNAAMVGFLEATRDDIAEKNRLCPPNPLPQVSLD
                     WVRGTLIGMNADYLWGKEPDIADWLERARLNIGRGLRRRSDDPLVQASSKRFGANARA
                     GLAKLTQFLR"
     gene            complement(15852..16568)
                     /locus_tag="VIG70_00200"
     CDS             complement(15852..16568)
                     /locus_tag="VIG70_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8249000.1"
                     /translation="MPLFGAALDAGRVTTGTRLLDAGCGAGLLALLANLRGAQVTALD
                     ASPGLLTIGRQRLPTADVREGDLEALPFADASFDAVTAVNSLFYAEDMATAMRELVRV
                     VRPGGRLIVTAWGPPHRCEFLTAVMPALGPLMPPPPPGAPPPHPGALSEPGALAALLK
                     GAGLRVVEEGEVACPFVFPSAEASWRGNASAGVNQAAIAHSGEAAVRAVYADADRAHT
                     RTDGTVRYENVFLWVAGERP"
     gene            complement(16765..>17071)
                     /locus_tag="VIG70_00205"
     CDS             complement(16765..>17071)
                     /locus_tag="VIG70_00205"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEY8249001.1"
                     /translation="VFHTDRGIEYAAGAFKERAAELGITQSMNRPGKVTDNAFIESFF
                     HSMKTDVYHGRSFDDDEEIRTVLKDYLPFYNRARVHSSLDYVSPATFEQQARGAGCQ"
BASE COUNT         2672 a         5868 c         5828 g         2703 t
ORIGIN      
        1 gacggcaagc tcgaggtgac ggcgaccggc aacgcggaca cccccatggc gcacggccac
       61 aagtgcctgc tgaccgtcga cgtctgggag cacgcctact acgtcgacta ccgcaacgag
      121 cgcgagcgct acgtctcggc gatcctcgag cgcctgaact gggaattcgc cgaaaagaac
      181 ctcggctagg gtttagcaga gaggattttc ccgacttgcg ggcggccggt gctcctcgga
      241 gaatcggccc ccgtgagggg gaacgggaac gaagaacagc aggactttgc cgccaagctg
      301 gcgctcatcg agagcgaggc gtacctggcg gcggagagcc tgccgccgag caccgtccgc
      361 gaccggatcc agcacatcgc ggtagtcgcg cagctgctga aggcgcgcct gaacgtcacc
      421 tccctgacca tccttcctcc ttcgcaacaa cccaggcgct cgaagacctg agcggaacct
      481 gaaccgctag tcagtcggcg acggccgcga atagcagggc aatcagggtg ccgagcccgg
      541 ccgggatgcc gtagcgctcc cagcgcgaca ccgcgttgcc ggcgatgatc tccaccggca
      601 cggaggtcag ctcgagcacc cggttggtcg tcgagctctc caccaggcgc gtgagggaat
      661 tcttgcgcgc cgcgctgagg acgatgtggt cgcaatgcag gcggtgcgcc gtcgcggtga
      721 tgcattccgc cctgtcgccg atctccatgt gcaccgcata gggaatgccg aagccgtcga
      781 ggatctggcg aaccggccgc agcgcctttt ccgcctcgtc ctggtggtag tcgcgcacgt
      841 tcctgcggct taggaagcgc gtgatgtagc tcgtgagcgg cggctgaacg ttgagcaggt
      901 ggatctcgat cgcggtgttg ttcatgaact gccgcacgac gtgccgcacc gcgaactcgc
      961 agttgcgcga gccgtcgacg ggaaccagga ccttgagcat ggcgttccct cccggggctt
     1021 ccagcggaaa gacgggttgc tcggattgcc tgcgcgcgcg gctgcggaag tagaacttga
     1081 gcagctccat tgcgatcgcc gaggcgaaga agaacggcgc catcatcagc cagtcgtcca
     1141 gcgtgagcgg caccgtgtcg aagaacggct gcaggaacgg cacgtagacc accatcagga
     1201 cgagcaagag cgacgcgccg accgcccaca ccatccagcg gttggagaac acgccgatcg
     1261 agaagatcga gtggtactcg gagcgcgccg tgaaggcccg caccagctcc gagacgcaca
     1321 gcgtgacgaa ggcgagggtc tgcgccgcct cgagatggcc ggggtagcgc tgcagcccga
     1381 gatagaacac cgtcaggatg gcgatcgcgt ccaccagcgc gatcacgccg atgccgatcg
     1441 ccatgtcgcg gttgatcacc ggctccttcg gcggccgggg cgggtgcgac atgatgtcct
     1501 tgtcgccttt ttccatcccc agcgcgagcg ccggcgcgcc gtcgctgacg aggttcagcc
     1561 acagcaggtg gaccggccgc agcgggatcg gcatgccgaa cagcatcgcg ccgaagatga
     1621 tcaggatctc gccgacgttg caggcgagca ggaagtacac gaacttgcgg atgttcgagt
     1681 agatgatgcg tccctgctcg atcgcggcga cgatgctggc gaagttgtcg tcggtgagga
     1741 ccatgtcggc ggtctgcttc gacacgtcgg tcccggtgat gcccatggcg acgccgatgt
     1801 tggcgcgctt gagcgcgggg gcgtcgttca cgccgtcgcc ggtcatggcc gccacgtggc
     1861 cgctcgcctt catcgcatcg acgatgcgcg tcttgtgctg cggcgagacg cgacagcaca
     1921 cctgcagctt gtcggtggac gccgcgagct cggcatcgct catgcgctcg atctcgggac
     1981 cggtcagcac cagcccgccg ggcgtgagga tgccgatctc gcgcgcgatg gcctcggccg
     2041 tgtccttgtg gtcgccggtg accatcacgc tcttcagccc cgcgccattc gccacgcgca
     2101 ccgcctcgat cacttcgggg cgcgccgggt cgatcatgcc gagcaggccg atgaaggtga
     2161 ggtccttctc gactgtgccc ggctggggat tctcgggcac ggcgtcgagc gggcggaccg
     2221 cgacgctcag cacgcgcagc gcgtgactgg cgaggtcgcg gttctggtcg aggatttcct
     2281 tcctcagggg gtcggtgatc gggaccgcct gaccgttttt catcacgtgc gtgcagaggt
     2341 cgagcaccac gtcgggcgcg cccttcacga acgcgaacgg cttgccctcg acggcgtgga
     2401 tcgtggtcat gcgcttacgc tcggagtcga acgggatctc ctgaacccgc ggcatgctct
     2461 tctcgagctc ggcgcgctgc aggccggcct tcaccgccac tacggccatg gcgccttcgg
     2521 tcggatcgcc gatgatgcgc ccctggtcga gcacggcgtc gttgcagagg agcgccccct
     2581 gcaggaggag cttcgcatcc gggtcggcgg ccgcctcgaa gcgcttgtcc tcgagcagga
     2641 agtgcccgct cgggtcgtag ccttcgcccg tgaccttgaa gcgcttgccg ccgctccacc
     2701 cctgcaccac ggtcatttcg ttctgcgtga gcgtgccggt cttgtccgag cagatcacgg
     2761 tggcgcaacc cagcgtctcg accgcgggca gcttgcggat gagcgcgttg cgcttgatca
     2821 tctgctgcat gccgagcgcg aggcagatcg tgacgatcgc cggcaggcct tccgggaccg
     2881 cggcgatcgc gaggctgacc gcggtcatga agaggttgat gatgtccttc tgctcggccc
     2941 gcagatactc gacgagcccg aggctgaaga tttccgtcag gcgggtgtcg cggaacaggc
     3001 cgtagaccag caccaccgcg cagatcgcga tgcacgcggt gccgagcacc ttgccgagct
     3061 gctgcagctt ctgctgcagc ggcgtgtcct cggcctcgaa cgactggatc atctcggcga
     3121 tcaggccgat ctgggtgttc atccccgtgc cggtcaccag gccgcggccg cggccgtagg
     3181 tcaccatcgt gcccatgaag gcggagttct tgcggtcgcc gaggggaatc tccgggtcga
     3241 gcacggccct ggcgttcttt tccaccggga ccgactcccc ggtgagggag gcttcctcca
     3301 ccttgagatt cacgctcgag accaggcgca ggtcggccgg gacgtagttg ccggcttcga
     3361 gcagcacgat gtcgcccggc accagctcgc gccccggaac cgtgacctgg tggttgtcgc
     3421 ggagcgcctg cgcgttcggc gcggccatct tcttcagggc ggcgagcgcc tgctcggcct
     3481 tcgattcctg gatcacgccg acgatggcgt tcagcaccac gatgcacatg atcgccaccg
     3541 agtcgaccca ttcgccgagc gccagcgcga tcagcgcggc gatgatcagg atgatgacca
     3601 ggtagttatt gaactggtcc ccgaggagcg cgaggaaccc gggcctcggt ttctcggtca
     3661 gctcgttcgc gccgaacttc tgcaggcgcg cgcgcgcctc ctcgccggtc aggccgtgct
     3721 cgaggtgcgc ctgcagagca gcgacgacct ggtcgatcgg cttggcatgg gcatgcgtgt
     3781 cctgcatatg acctcccgcg attggcgacg cgggaactat gcggcggtgc agcggcgaag
     3841 gaaatttgaa ccttgtgatg cagagcatta cgtttcgtaa tgcccagggg ttcttgaaag
     3901 ccggccgttt cggtgctatt actctcctac cccaaagggg agtagcttgg gcggccgccc
     3961 ggccgccgtg gcgcgatcgt caagaacggg ctgaaagccc cggtcgcgtc ggcatcgccg
     4021 tcgaatgcga tgcgagcgag acctttgccg aagaggcgaa ggactcactc gaggagcgat
     4081 gccatgacgc acacgccgga catccgacgc tacctgaagc acggcacctt gccgcaacta
     4141 cgcgcgttcg aggcgagcgt gcgcctggga agcttcaccc gcgccgccgg cgagctgcac
     4201 atggcgcaag ccacggcatc ggtgcagatc aagaagctcg gcgagaccgt ggggctcgcg
     4261 ctgttcgagt acgtcggcaa gcgcattcgc ctgaccgacg ccggcgagcg ggtgtacgcc
     4321 ggctgcggcg aggtgttccg cgcgctttcc gacatggagc ggggcctgat cggactgcgc
     4381 ggcgccgcct gcgggcatct ccaggtcgcg gtgccgtcga gcgcgcgcta tttcgccggc
     4441 cggctgctcg acgccttcct gcagcataac cccgacgttc aagcctcgct gcaggtccgc
     4501 gaccgcatcg ccctcgccga acacgatctc tgcatgctgg tcgagccgcc gccggggcag
     4561 gaagtggtcg cgcaggcgct agtcccgaac cccctggtcg tcctggcggc ggccgatcac
     4621 ccgctcgcgc gggataggga catccctttc gagcggctgc agcacgagcc ttttgtcatg
     4681 cgcgagcccg ggtcgcccac gcgcatgctc gtgctgcgct tcttcgcgcg ccacggcatc
     4741 gtcccgaaga gccgcatcga gttctcttcc gacgaggcgg tgcgggaggc gatctgcgcc
     4801 gggctgggca tctcgatcct gccgcgctat gccgtgggca tggacccggc gctcgcgcgc
     4861 ctcgcgtgcc tggacgttca gggcttcccg ctggaggcgc actggcactt cgtctacccg
     4921 gtgggcaggc gtctttccgc ggccgcgcgc gcattcatgg acttctcgcg cgccgaagcg
     4981 aagagcctgt ttcgcgactg tctgcgcgaa gcgaactagc tcagacggcg gcgaggggcg
     5041 cgcgggggtc gaagtcgcgc caggcgccgt cggcccactc gccctcggcc cggcgcacgt
     5101 cgcgcgcgct gtggcccctg aggatctcga tcgcacgcct ctccgtgccc gcgcggtcga
     5161 cgttgatcgc gatcatgcgg ccgccgcgcg gctcgaccgg gtgctcgacc gaggcgtcgg
     5221 cgcggtgccc gccgcgcacc cgcgccatcg agccggcgaa cgagccgaca taggcgccga
     5281 ggcctgcgcc gagggggatc accaccagcc cgaggtcgcc cgacaccgcc cagccggcgg
     5341 cggcgccgag cacggcgccg accgcggcgc cgatgccggc gccgatgccc gcggtgctcg
     5401 agccctcgct cgacgacgag tcgccgccca gcggcgtcat ggcgtgctgt cccggcggcg
     5461 agacgtagaa ggcgtcgatc tcggcgcgct ggaagcctgc gcgcttgagc tcctcgagcg
     5521 ccgcgtcggc gtcgatggtc cggtcgaaca ttccacagat gatgcgggac atttagggcc
     5581 ttcctttttc taggcgcggt tatcggcgcg gcgcacgtcc acgctcggca tgcgcatcaa
     5641 cgctcggtac ttggtgatcg tgcggcgggc cacgcgcagc ccctgctggg caagcagcct
     5701 cgccagctcg gcatccgaca gcggcgagcg cgggtcctcg gcggcgatca gctccttcat
     5761 caccgcgcgc accgcggtgg ccgaggccgc gccgccgttc tcggtggcga ggcgccggga
     5821 gaagaagtgc ttgaactcga acaggccgcg cggcgtcgcc atgtacttgc tgttggtgac
     5881 gcggcacacg gtcgactcgt gcaggccgag ctcgcccgcg atcagtttca gggtgagcgg
     5941 cttcatcgcc acctcgccgt actcgaagaa gccgcgctgg cgcgcgacga tcgcgtcggc
     6001 gacgcgctgg atggtggcga agcgctgctc gatcgagcgc agcagccagc gcgcctcctg
     6061 caggtggcgc gcgagcgact ggttggcgcc gttgcgcccg ccgatcgcgt cggcgtagct
     6121 gcggttcagc cgcagccgcg gcagcgacgc gggattgatg agcgccaccc agcggtcgcg
     6181 gttcttgcgc acgatgacgt ccgggatgac gtagcgcgcc tcgttcgcgc cgaagcggtg
     6241 gcccgggcgc ggatcgagcg agcggatgag cgtgcgcgcg gcgtgcagct cctgctcgct
     6301 gcagtcgatc gcgtgctgga gacgggccca ttcgcggttg ccgagcaggc tgagatggtt
     6361 cgtcatggcc agcgccgcgg cgcggctcgg ggtttcggcc gggaggttct cgagctgaag
     6421 cctcaggcat tcatccaggg agcgcgctcc cactcccgcc gggtcgaggc tctgcacgag
     6481 cttgagcgct gcggcgaact cctccggcag cacgtcgtgc tccttcggca gcagcgcggc
     6541 gagctcctcg aagctcgcct tgaaatagcc gtcgtcgtcg aggctgtcga tcaccatctc
     6601 ggcgagcgcg cggtcgcggt cgccgacgcg cgacatgcgt agctgctcgc gcaggtgctc
     6661 ctgcagcgag ggctgcgctt cggacgcgcc gccccactcg tcctgctcgc gctccagggc
     6721 cgcgccgccg cccgagctct cctgcggcag catgacgtcc ccgaccgggc cgtcgccgct
     6781 tttcgttccc ggcaaaaccg ccggcgcgtc cgggttctcc tcgaggaaag ggttggcggt
     6841 gatcgcctgc tccatttcct gcgcgaactc gagcgccgac atctgcagca gccgcagcgc
     6901 ctgctggacc tgcggtgtaa gcgtgaggtg ttgccgcaga cgtaatgcga ttgccggggc
     6961 catggttttc ctttcccggc aaagtctgca aagggtatgc cagcgcgctt gctcctaagg
     7021 gcgcacggga tttggctgta atacttgcgc cgcgcgcgta gctattaccc ttccggcctg
     7081 cgccttgcag gcgggcttcg catcgcaaac atcatcacta gcgccgtacc acgctgacgg
     7141 tgccgtcggg ctccatatag gcgctcgcga cctggcgcac gtcgtccacg cctttttcgc
     7201 gcagcttcga cttcagctct tcttccgtca cgaactcgtg gcgcaggttg cggtacagga
     7261 tgcggccgtc gtcgaccagc agcagcggcg gcgcctcgag ccacgactgc agcgcaggga
     7321 ccttgaagtt gagccagtcg atgagccagc tccagccgat gatggtgccg atgaggatcg
     7381 cgccctcggt caccgagcgg tactcgcccg ccatgccgtt ctgggcggcg tcggcgatga
     7441 tcacgaggac caggatgtcg gccatgccca ccgcgccgat gcgccgccgc accaccacgc
     7501 ggaagatgag gaagaggaac aggtacatcg cggtgccgcg cacgacgagc tcgagcggcg
     7561 acacggtcag gccgaagatc gcgccccagt ccaggctcac ggccgcgctt ctccttcgta
     7621 gctaacgggg aaataccgcc gcaggcgcga tgcggtgggg aggggaggag acgcagccac
     7681 gcccgggcgg gtgttcccct catgcatacc gcgtgcctgc ttttcagccg acgccagggg
     7741 acgacgcggc ccgcggcttt ttcggccgaa cttgcgcagc ggtccgcgtc gccgcgaccg
     7801 gaaccgcgcc gccttcgcct tcccagactt ccagcttctt ttccgtgaga cggtttttgc
     7861 tcttctcatt caagaagatg aaaaagtggc gggcgccgca ggcgacgccg gcgagcgcgg
     7921 cgatggtcag gatggatctc atggtgtcgg ggtggtacgc aagttgcgtg ccgttcttac
     7981 atccccttct tgtaagcctt tccaatgccc catctgctgc tcgtagacga cgaccccgaa
     8041 gcgctcgaat ggctgtccga gttcgccaag gccgaagggt tcaccgtatc gcaggccgat
     8101 tcgctgcgcg ctgcgcgcat ccacatgtct cggctgcagc ccgacgtgct gctgacggac
     8161 ctgcagcttc cggacggcaa gggcgtcgag ctggtcgccg acctcgagca gcgcgaatcc
     8221 accgaggtgg tgatgattac cggtcacgcc agcgtcgaga gcgtggtcga ggcgctgcgc
     8281 ctgggcgcga gcgactacct ggtgaagccg gtcgacacgg agcggctgaa atccatcctg
     8341 cgccgcgtcc cgcgtaccgg cgagctgcgc gccgagatcg gcgagctgcg cgacgagctg
     8401 cgcaagctcg gccgcttcgg ccacatcctc ggctcctcga cgccgatgca gaagctctac
     8461 gaccagcttg gtcgtgtggc gccgacatcc gcgacggtcc tgctcaccgg cgagagcggc
     8521 accggcaagg agctcgccgc gcaaaccatt catgatttga gcagaaggaa gaaggcgccg
     8581 ttcctgccgc tcaactgcgg cgcggtgtcg ccgcaattga tcgagagcga gctcttcggc
     8641 cacgagaagg gctcgttcac cggcgccgac cggcagcaca agggctactt cgagcgcgcg
     8701 cacggcggga cgctgttcct cgacgagatc accgagatgc cgcaggagct gcaggtgaag
     8761 ctgctgcgcg tcctcgagac cgggaccttc acgcgcgtcg gcaccacctc gccgatcagc
     8821 accgacgtgc gcgtgctcgc cgcgaccaac cgcagccccg agggcgcggt cgccgacggc
     8881 aagctgcgcg aggacctcta ccaccgcctg aacgtgttcc cgatcggcat gccgccgctg
     8941 cgcgagcgcg ccacggacat cgagctgctc gccgagcatt tcctcggaat cctcaacagc
     9001 caggagagcg ccaccaaatc gttcgccccc gcgaccgtgg cggcgctcta cgcgcatagc
     9061 tggccgggca acgtgcgcga gctgaagaac tacgtgcagc gcgccttcat cctggccgac
     9121 gacgtgattg attcgaattt ggcacctaca gtagtaactc aacaggaaag cgccccgctg
     9181 ctgtcggtgc gggtgggcac cacgctcgac gacgtgagcc gccgcctgat cgaggcgacc
     9241 ctcgccgaat gcgggtcgaa aagaaaggcg gccgacatgc tcggcatcag cctgaagacc
     9301 ctctacaaca ggctcgcggc ctacaagacc gaaaccggcg aagagcgcga cgccgcatga
     9361 cgccgaagcg gcgcgacgag tcgcgcgtga aatcgaacta cctgcgccgc cggcgcgtgg
     9421 cccaggccgc caccgatgtg acgcgcggcc tgaaagacac cgagcggcgc ggcatcccga
     9481 acgacgtccc ggcgcctcgg cggcggcggg tatagcagtt gcttttgcaa cacccgaaac
     9541 ccccgaacca ctgaaccaac ggagatcccc atggcaagta ccccgagcag cagcacccag
     9601 aagcacatcg agcgcgccgg cgacagcgcc cacgacgtcg tcgaccgcgt cgccgagacc
     9661 gcgagctcct acgccgagcg cttcggcgaa cgcgccgatg agctgctcga gatgaaggac
     9721 aactgggtgg aaggcgcgcg cgagtacgtg cacgagcacc cggtcgcggc gctgggcatc
     9781 gcggtcgccg cggggtacat cctgagcatg ctgatgcggt cccgcgatta gtgtgaacag
     9841 tttgcagcag ccgcggccgc gtcccccggg cctcggcgag ctggtcggca ggcttctcgg
     9901 cgaggcgcgc agcctcgctg ccgatttcgc cgagctcgcc gtgctcgacg cgcgccgcgc
     9961 ggcgatccgg ctgtcgtggc tgctcgccgc ggtgctggtg gtcacggtgc tcgtcgtcac
    10021 cacctggatg gcgctggtcg ccgccggggt gatcctgatg ctcggccagg gtgcctcctg
    10081 gataatcgcg ctggcggtcg ccgccgtcct gaacctcgtc ggcgctggcg cgctggcgtg
    10141 gtggatgttc gcgctgatca aggagctgcc gttcacggcg ctgctgcgcc agctgcgcgg
    10201 cgacgctccg cagaaacccc aatgattcct cccgacagcg agattttcga ggtcgagcag
    10261 cgcatcgccg ctcgccgtgc ccaggtgaag cggcacggcc gcgaagcggg cacgcgcgcg
    10321 ttgcagtcgc tcgcttcgcc ggcggcgctg atcggcgccg ccgcgctcgg cttcttcacg
    10381 gccaccgcgt tcgccaggcg ccgcgagaag ccgaagcatc cggagcggcg caagagcgac
    10441 cacaccaagg ccgccaaggc gaccggcctc gccggcgtgc tgctgccggc ggcgatgtgg
    10501 ttcgtgcgcg cgcagtgggg ctcgccggtg cggctcgcgc aggtgatgct cgaaaaattc
    10561 cagcatcgaa atgcgccgac gtcgcttcgc ggcgacacgc ggcgtgacac gagacgcatc
    10621 gctgccccgt agctattcgg gcgatattca ttgaccctcg ggggcgctag tctcgccccg
    10681 atggaaattc tgtcgcgcct gacccggagc gcgcacgccg cgttcggcct ggtcgcggcg
    10741 cttgccgcga tggcgctgtg gggctgggcg gtccatgtcc cggtgctgcg cgatctcggc
    10801 gccgacttcg cgccgatgtc ggccgcggcg gcgttcgcgt tccttctgat cgccgggagc
    10861 ttcctcgccg ccgagcgcgg ccgccaggct tcggcgtatg tcgctgccgg gctcgccgcg
    10921 gcgctcgcga ttgccgcgct cgtcgagcac tacctggcgc tgtccctgaa cctgcagatc
    10981 gcgcccgcgg cgagcgtcac gcttctcgtg ctcgcgctcg tgaccccgct gccgcgcggc
    11041 gccaaagccc tcggcgtccc gctgcagggc gccgccgcgg cgctgatcgg cggggtggcg
    11101 ttcttcgcgc tgctagggct ctcgctgcgc gtcctgcgtt tcgacattcc cgcgccgctg
    11161 ctcggattct cggccccggg cgcgctcgcc gcgatgctgg cggcggccgg cctcgccgcc
    11221 gcgcggccca ccgtgtggct gctcgagacg ctcgccgggc ggcgcaccgg cgcggtggtg
    11281 acgcgctggc tgctgccggc ggcgttcatc gtgccgctgg cggtcgggtg gacgcggctg
    11341 ctcgccgagc gcgaagggct gttcggcgag gcggtgggca tggcgctctt caccacgatg
    11401 atgatcgcgg gcttcggcgc gctgatcctg tgggtggcgc gcaccctcga ccatcacgag
    11461 cgcaagcgcg cgcaggccga ggagcaggcg ggcgagacgc gcgaatggct gcaggtcacg
    11521 ctggcggcga tcggcgacgg cgtcatcgcc accgaccgcg cggggcgcgt gcgcttcctc
    11581 aacgccgccg cgcagcgcct gaccggatgg cgcgcggccg aggcggccgg gcggccgctc
    11641 gacgagctgc tgcagctctt cgacgagcgt aacggcgccg cgctcgccag ccccctgaat
    11701 tcctgcctca agacgcggag catctcggcc gcgaacgggg agccggcgct gcgcgcgcgc
    11761 gacggcaagg tgcatccggt gcacgccaac gccgcgccga tcgtcgactc gggcgatggc
    11821 atcgccggcg cggtgctcgt gctgcgcgag gcggcggcgc agcgccaggc cgagcgcgcg
    11881 atgcgcgagg cgtacaccga gctcgacgag cgcgtcgtgc gccgcaccgc cgcgctggag
    11941 cgcgccagca ccgcgctgcg ggagcgcaac gcgctcctga acgcgatcac caccagcacg
    12001 cccgacctga tcttcgccaa ggaccgcgag ggccgcatgc tgatggcgaa cccggcgtgg
    12061 ctgaccgcgg tcgggcgcgt gcgcgaggac gagcgtcccg aggtgaccgg cctcgacgac
    12121 aacgagcgcc tggtgctcga ggccggcgag accatgatcc tcgaggagaa cgtggccgag
    12181 cgcacctacc tcgccaccaa gtcgccgctg cgcgacgagc acggccgcgt gatcggcctg
    12241 atcggcgtcg ccaccgacat caccgagcgc aagcaggcgc agcgcgacct ggagaagctg
    12301 gtggtggccg agcagcggct gcgcgccgag gccgagcgcg ccaaccgcgc caaggacgag
    12361 ttcctcgcca tcgtgtcgca cgagctgcgc tcgccgctca acgcgctgcg cggctggagc
    12421 cacctgctcg ccacgacacg ccctctggaa cagcccctgg ttgaaagggc ttcgaaagcc
    12481 atcaagcgca acgtcgacca ccagacgcgc ctgatcgacg acctgctcga cacttccagg
    12541 atcgtgagcg gcaagctgac catcgagcgg cgtttggtga acctggtcga gatcgtgctc
    12601 gccgcgctcg acgcggcgcg cccgggcgcc gcgtcgaaga aaatcgacct gcgcttcacg
    12661 cccgacgacg ccagcatcat ggtggtgggc gacggcggcc ggctgcagca gctcatctcc
    12721 aacctgctgt ccaacgcgct gaagttcacc cccgagcgcg gcgccatcgc cgtgctgctg
    12781 ctgaagaacg cggagcgcgt gcagctcctc gtcaaggaca acggcatcgg catcgcgccg
    12841 gaattcctgc cgcacgtctt cgaccgcttc acccaggccg acacctccgc cgcgcggcgc
    12901 gcgggcgggc tggggatcgg cctggcgctg gtgcgccaca tcgcgctcct gcacggcggc
    12961 caggtgcgcg ccgacagccc gggcgtcggc cgcggctcgg ccttcaccat cgagctgcct
    13021 gccgcgccgg cgagcgcgct gcgcgcgccg gcgccgcgtc cggagcgccg cggaacgaac
    13081 gagggcgccc tcggcggcgt gcgcatctgg atcctcgacg acgaccccga cgcgcacgag
    13141 gtcgcctccc tcaccctgcg ccagagcggc gcgctggtac agagcgcgca ctcggcctcc
    13201 gagctcgccg cgatgctcga gcgcgccctg ccggccgagg gtcccgacgt gctcctcgtc
    13261 gacctcgcca tgccgggcga ggacggcttc gaggcgctgc gccgcgtgcg ctcgctcgaa
    13321 atggcgtccg gctccgagcg ctacatcccc gcgctcgcgc tgaccgcgtt cacccaggtc
    13381 gagcgcgaga agctcgtcgc cgcgggcttc gccgaccgcg tcgacaagcc gatcgacgcc
    13441 gacaagctcg tcgccgcgat ccgcagcgcc ctgcacgaga accgccgcaa cgccatgcgc
    13501 gccgcctgaa ttcggggacg gagccctaat tggttcgggc tccgtccccg aactagcgta
    13561 gaaactgcgt cagcttggcg agccccgcac gcgcattggc gccgaagcgc ttcgaggacg
    13621 cctgcacgag cggatcgtcg ctgcggcgcc gcaggccgcg accgatgttg agccgggcgc
    13681 gctcgagcca gtccgcgatg tccggctcct ttccccacag gtagtccgcg ttcatcccga
    13741 tgagcgtgcc ccggacccag tcgagcgaca cctgcggcaa ggggttcggc gggcagaggc
    13801 ggttcttctc ggcgatgtcg tcgcgcgtcg cttcgaggaa gccgaccatc gccgcgttga
    13861 aggggacgag gccgggccgg atcggctgca gcgtgatgcg gtcgttcgcg aagatgggaa
    13921 tgccgggagc gggattgagg ccgcgcgcgg cgcagtggac atgcagatgg tccgggctcg
    13981 tcgcgacggt tccctggtcc agcagaatcg agtcgcgctc gacgcgccgc acgtatccga
    14041 gccgcacgac gttctcgatg cgggcgagct ctgtgatttc gcccgcggtc acggtggcgc
    14101 tggtgtactt ggcgggcttg gcgctgccgt cgacgcggag caattggccc gccgcgttga
    14161 gccggcggaa caggtcgtcg agcgacgtcg cttgcgcggc ggcttcgagc tggagcgaga
    14221 gcccctcgat gaacttgccc acctgctccc cgccctgcag gaacacgcga ttggcgagcc
    14281 aggactcgcg cggctggatc cagcggatgt cgccgggcgg gacaccgatc tccagcagcc
    14341 acaggcacgc gtcgatcgcc gtcttgccgg cgccgatgat cacgtagccc cgggcctgcg
    14401 gcgccatccg gggaagatcg ttgacgggca cgcagcgcgc tccttgcgcg acctcgaacg
    14461 gcggcgggca gctcgcgggc accgaaggct ggagataggc ggcgtcgacc cgcttcttct
    14521 tcaccttcac ttcgaatcgc tcgcccgaga cgcgcgacac gaaccggtgc tcgccgacgt
    14581 agtcgcacat cgggaagtag cgcacctgac cggagggcag caggcgctgc tgcatgacgc
    14641 ggtcgtagta cccgcagatc tcggcgccgc tcgcccgctc atacaggccc tggttcagcc
    14701 cgtggcggtc gatcgtatcg ccgccgagcg gcgtggagtt cacgccgtag tacgcggagg
    14761 gctggtgcag gcgcaggaac ggatagccct cgttccagtg gccgcccggc gcgtggcgcc
    14821 ggtcgaccat gatgacctgt gccttggaat cggcgatcag cgaatccgtg aacgccatcc
    14881 cggccgcgcc gcagccgacc acgaggtagt ccgcttcgag ggttcgcgtg ctcatgattc
    14941 gcttgaggtt agcgcgccgg ctcccgcaga ctacttcgag caggcgccat agaacccgca
    15001 cccaagaagg aacgtgatga cgatcgtggt cagaagaccg gcgacgaaca atcctgcgct
    15061 tgccgccaag cgatgcccat gggaaggata ggcgatggct tgaacgaagc acaccgctgc
    15121 ggcagacggc aggcagagcg ttgcaagcgt tatgtctggc gttttccgcg tgactgacga
    15181 aggtgtgaaa gcgtagtgcg tgagagcgat ggaacctaca gcaacgacaa cgagtgcagc
    15241 cagccatacg gtgcgcggtg tcggacgcca catagcgata cctgcagtct aacgttcgcg
    15301 ttgaagggcg cgcgaccacg gacgcaaact gagcgcgtgt tgcttgtttg cgtatccgct
    15361 ctcgaacgca tggctaggcg ccacgcttcg ctctcccgag gtaccgacgt gccgctgctg
    15421 aatcaatgag cgggtaaaga tcgaaatgag caggaatgat gtctgcccag ccattcagca
    15481 gcgcgtgtaa ctcttcgttt gacctcacat caaatagtgc aaccgcgccg cgcccggtgc
    15541 gtgcgtggac aaggcgacat tgcctcgacc ttagctttgg atcaatccag cgccagaatt
    15601 tccttctgct tgccaatacg gtcgatgggc gctccggccg cggggtgcta atgactagaa
    15661 agagcacgca aacctcccca tccgttcggc cgtcattgtt tgtggcacct aacgttccgg
    15721 ttcagcggcg ggccgcgtgg cggaccgtcc gctgcaaccg gttgttagca tgcacgattc
    15781 cacaatccga agcgccgccg catgcgccag gacaccccga taggatggcg ctgcgttggc
    15841 gacgtccttc gctacggtcg ttcgcccgcc acccacagga agacgttctc gtaacggaca
    15901 gttccatccg tacgcgtgtg ggcacggtct gcatccgcgt agacggcacg aaccgcggcc
    15961 tccccgctgt gcgcgatggc cgcctggttg acgccggcgc tggcatttcc gcgccacgat
    16021 gcctcggcgc tggggaagac aaagggacag gcgacctctc cttcctccac gacacgcaag
    16081 cctgccccct tcagaagggc cgctaacgct cccggctcgg acaaggctcc cgggtgcgga
    16141 ggtggagcgc cgggcggcgg gggcggcatg agcggcccga gcgcaggcat cacggcggtt
    16201 agaaactcgc accggtgcgg cggtccccaa gcggtcacga tgagccgccc gccgggccgc
    16261 accacgcgga cgagctcgcg catggcagta gccatgtctt ccgcatagaa gagactgttc
    16321 accgccgtga ccgcgtcgaa gctggcgtcg gcgaagggca gagcttccag atcgccctcc
    16381 cgcacatcgg ctgtgggcaa acgttgccgc cctatggtaa gcagccccgg cgaggcgtcg
    16441 agggcggtca cctgcgcgcc gcgtaggttg gccagcagcg cgaggagtcc tgcgccacac
    16501 ccggcatcga gcaggcgcgt gccggtggtc acacggccgg catcgagggc cgcgccgaaa
    16561 agcggcaagc acacctgctc cacgtaggcc gcccagtcct gagcacgcgc cccccaaagt
    16621 tgaccttgaa tcgttgcgct acccataagc acgtcctttc tctatggtga tccaactcca
    16681 aaggcatgct tcgagacgcg ttggtgcatg ctaacgtccg gcttcaggcg cgcgccgctc
    16741 gcggcgcggg atcttcccct gatttcattg acacccggct cctcgagcct gctgttcgaa
    16801 cgtcgcaggc gacacgtagt ccagtgacga gtgcactcta gcgcggttgt aaaagggcaa
    16861 gtagtccttc agcacagtcc ggatctcctc gtcgtcatcg aagctacggc catgatagac
    16921 gtcggtcttc atggaatgga agaacgactc gatgaacgcg ttgtcggtca ctttacccgg
    16981 tcggttcatg ctctgtgtga tgcctagctc ggcagcgcgc tccttgaagg cgccagccgc
    17041 gtactcaatg ccccgatcgg tatggaacac c
//
LOCUS       DATBSZ010000004        13424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_478702, whole genome shotgun sequence.
ACCESSION   DATBSZ010000004 DATBSZ010000000
VERSION     DATBSZ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13424
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478702"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VIG70_00210"
     CDS             <1..688
                     /locus_tag="VIG70_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEY8249002.1"
                     /translation="YNVNEDFSQANDLASKHPEKLKELQAVFMKEAVRNHVLPIDDRR
                     AERFNPAIAGRPDLLDGRKTLTVYPGMTGMLENAFINVKGVRHTITAEVDLKDARTQG
                     VIIAQAGYFGGWVLYMKDGKVRHEYNFFGLERTNIASSEALAPGKQTIVYEFIPDEAK
                     AGTGGRSILRVGGKQVAEGKIPKTQPFVFSADEGTDVGLDGETNVSTDYKQGNNAFTG
                     RIGKVTVSVE"
     gene            695..1675
                     /locus_tag="VIG70_00215"
     CDS             695..1675
                     /locus_tag="VIG70_00215"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8249003.1"
                     /translation="MRALVAFLSLLTLAANVHGADTLPSWNEGATKSAIVDFVARVTK
                     KGGKDYVPPAQRIATFDNDGTLWAEQPLYFQFLFALERVKAIAPQHPEWKDKEPFASL
                     LKGDLKGALAGGEHALLEIVAETHSGMTTEEFDRIVADWLRTARHPRFKRPYNELVYQ
                     PMLELLRYLRANGFKTYIASGGGVEFMRVFAERAYGIPPEQVIGSSGKTKFELRDGKP
                     VLVKLPGLDFLDDKEGKPINIQKIIGRRPLAAFGNSDGDLQMLQWTCAGPGARFCLFV
                     HHTDEAREWAYDRASHIGRFDKGLDEARAKGWTVVDMKRDWKVVFPFEKK"
     gene            1685..3301
                     /locus_tag="VIG70_00220"
     CDS             1685..3301
                     /locus_tag="VIG70_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEY8249004.1"
                     /translation="MKRALAFLITAGAALLFAGAAGAQQAKRPNIVVIFGDDIGMWNV
                     GAYTHGMMGRTPSIDSIAREGMLFTDHYAQPSCTAGRAAFITGQLPIRTGMTTIGIPG
                     STRGIQKTDPTLATVLKTQGYATGQFGKNHLGDRNEFLPTVHGFDEWFGNLYHLNAEE
                     EPEQLDYPGQKNPEYLKRMGPRGVLHAWATDKDDSTTDPKFGRVGRQRVENTGPLTRK
                     RMETFDAEVLGHTLKWLDGVKDKPFFLWFNTTAIHIHSHSPRKYIQMAVDEGRGEEDV
                     VRAKMIEHDEQVGAILKKLADLGVADNTIVIYTTDNGFEFMLWPDGGYAPFRGEKGST
                     WEGGVRVPFLVKWPGRIKPGSVSNGIQNHEDVFATLAAAAGLPDLKKELMAGKKMGAT
                     SYKVHLDGYNNLAHWTGASEKSARREIFYYDETDLMALRVDAWKMHIGAKKEGIWWNE
                     KHYPSVPYLFNLLMDPMEKMDQESHEWGAAARRFFASKMWALNSSAPFIQAHLKTLMD
                     YPPSQGADTLSMKRALDEVMRKLEKPQTAR"
     gene            3476..5302
                     /locus_tag="VIG70_00225"
     CDS             3476..5302
                     /locus_tag="VIG70_00225"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY8249005.1"
                     /translation="MAEEAAAGYLAGRIDAVREHLSSLAAAAPRLGAEFGKVGDELAR
                     EWQTRAPGSILALIAACVLLGYLVRAGYHYWVRGAEVRDATVRERLRSIGLRFAREVG
                     GVAAFAIGSAAVVLIFEWPARTREAVLGFLAAFVVVWLVIVASRALLSPREARLRILP
                     ASDPEAAFWHSRIVLFASWFAFGWVIKSWLLLLGMDLPALRLVIYALGLGLLAIAIEA
                     FWRRSRLAALYFVLLWVLWSMRAFGALWLAVVAAALPVVMRLVQRGADHVLRPPGTAA
                     AEARGVAAATIGRALRALAIIGAAYLLVDAFGLDVDALAASDAMGMRLARGAVHALVI
                     VLIADVAWMVCAALIDQKLASVRTSTSPENEHRDGRLRTLLPIVRGTLAVVLIVMTAL
                     MVLSALGIQVGPLLAGAGVLGLAVGFGAQTLVRDMFSKFFYLLDDAFRIGEYIQSGNY
                     KGTVEHLGARSIRLRHHRGPVYTIPYGILGAVQNMSRDWVIDKLQIGITYDSDLEKAR
                     KLIKKIGQELAQDPALAPNILETLKMQGVEDFGDFAIKIRMKMMTKPGEQFVIRRKAF
                     GMIKKAFDANGIKFAYPTVQVAGPAEGAVAAAAAQQALKPAP"
     gene            5315..5692
                     /locus_tag="VIG70_00230"
     CDS             5315..5692
                     /locus_tag="VIG70_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249006.1"
                     /translation="MLRIVIVAATLGLASTQAAAQAWIGLLKNTPAERFQEDDLQMFL
                     EHARKALNEAPDNQPVSWENPKTRARGDVTVMRSFEYKGLPCKELRVRNEAQGRKGDN
                     TFNLCNADGKWRLLSSSQLKSKK"
     gene            5769..7529
                     /locus_tag="VIG70_00235"
     CDS             5769..7529
                     /locus_tag="VIG70_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HEY8249007.1"
                     /translation="MLRRCVRAAAVACAALACAAALAQEPGSTGPKYRLTVEAPTKEL
                     REMLSKGLQLSRWQDDREMTPELLRRLADEGVREAREALAAQGYFAALVSFSLDRDRE
                     PWALTLHVQPGPRTVVRTTEILFTGPGATDPEAAGLLKQVRRDWLLRPGQPFTQEAWN
                     EAKREVVKSLSSWRYAAARLASSRARVDPETSSAALEVTLDSGPAFVFGGVEVRGTKR
                     YREEVAGHLSPVEPGATYDRQVLELYTRRLLETGYFASARVDILPEGREGGAAPVRAS
                     VIEGNSQHFEGGVSYNTDAGPRLELSHRNVDLFNTAWRGKSVLRIDQLTQEASYNLDS
                     PPRRGATWWTGTTGAKHSIIQEEENTELSLGVAHNWAGWGSPTAVLVSAVAEEQRISG
                     EAADHRHAIYFGIRIGFRDTDDIVLPRRGYFGQVTAGGAPDELSTRGFQRGTARITLM
                     HSLGRNDDLLLRAEGGIVVARSRQGIPSTFMFRTGGDQTVRGYAFESLGVRQGDAVLG
                     GRYLAIGSVEYTHWFAPAWGLAAFVDGGNAWDTGDYHPVFGYGIGGRFRTPIGPVRLD
                     VAYGEEERSWRLHFSVGFVF"
     gene            7536..10910
                     /locus_tag="VIG70_00240"
     CDS             7536..10910
                     /locus_tag="VIG70_00240"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY8249008.1"
                     /translation="MRWLKWLRWPLRGAVALALAAAAVVVWVLGTEGGLRWALGFVPP
                     EIELEAPRGALWRTVSFDRVAFQGSEARSVALEVDLLALFSDTIAVEFLRIDSLTLRR
                     PEGSSEKSETPLPFRIRVADAQVKSLVFEGYEIHDLQAEYSGSASGHEAQASFSAAGA
                     RARAKVVFDKEISIEADVKAINLAVIDPGLPQTALNASLTARGSTSALAGTLTVANPN
                     PGPIDRERLPFSRIAAAFGTDFKLLTLEKLNAQLHPAGTVQGKGSASLEGAKFDIRIA
                     ALDLRALYSSLRATHLAGALELDLARERQRVKGTLTQEDMSLTADAERKGDDVEVRAL
                     RALAGDSEASGSGRIHLGEPLRFAADLKLTRFNPARFGDYPEGSINGTVQGKGDLNGR
                     GSGRWEIVDSTLFGEKLASRGAATLVGERIVAADAWATLGENRATAKGSFGGPQDRVA
                     WTLHVPDLAALAPDFGGEIRASGTATGTWKQPSVVLDAQATTLRLAEALVFDRATVKA
                     SGTLERHEGNLTAKNEEFDLKATLRGGWRQNAWRGEIASLTNAGAYPLELKTPAALEA
                     GAARVVLGRFEAVLAGGRAAVESVRWEEKRLTSSGRISALPAQWILTALDVDKVKGDL
                     ALDGDWELASTPRLNGRVAVRRAGGDLAIGEVAMELASASVQATFTDDRVEATAEAVS
                     RLVKVQAKGTAAGLTPDSALALSAEIEAAELRSLTEPLWTQARVSGRVGASLKVEGTP
                     RAPVVSGTLRGDALGVEMPVWGVALKDGRVRAELDANRLLVKEARIAAGDGSFSASGT
                     LPLGGDGAASLDWQAQQFRVLSRPDRRLVVSGKGVTTFDGKRFGLVGELRADSGRFEL
                     AADTLPTLDDDVEVVGQKADEIRLTTRKQGPLPLDLDLKVDLGNRLTVRAFGYDGGVA
                     GKLRVATNPAGELIAQGRIEAVKARFRAYGQELQVDPGIVIFDGPFDRAGLDITAWRR
                     HQQVEAGIRLTGTLQTPRVELISNPPVAENEKLSWLVLGRAPTDANGADLAVLQAAGG
                     AVFGRGGDEPFNRKFAQRFGFDELTVRSSSELASNVVALGKRYSDKVYFSFEQAIGTT
                     TEYLVKLDYALTQRVSLRGQTGTTSGVGLFYRYSWD"
     gene            complement(10907..11209)
                     /locus_tag="VIG70_00245"
     CDS             complement(10907..11209)
                     /locus_tag="VIG70_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249009.1"
                     /translation="MLEWAMKSTPIWLAVFALAACAAPVAQWEKSGADQSTTSLDTQQ
                     CREQARLAQPPPLQARAPDFSAQSVMSNDMREDREVREAQQFQLCMREKGYVSVRR"
     gene            complement(11226..11798)
                     /locus_tag="VIG70_00250"
     CDS             complement(11226..11798)
                     /locus_tag="VIG70_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249010.1"
                     /translation="MLDMLDCALLIADDEGRVEYRNCVASTLLARGHGGLTLTAGTLS
                     AKVAKVRDALAQAIRLACDGLQPSGLCLPQPGTPPERWLRMVVAPVYFGGAAGGASRA
                     AVWILNTEAPALPSEELLSALFGLSRAEARLAIGLLMGRTAAEHARHAGVGVATIRSQ
                     LHRIFSKTGVRRQAQLVALLLKVPVVQLGR"
     gene            12003..12713
                     /locus_tag="VIG70_00255"
     CDS             12003..12713
                     /locus_tag="VIG70_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8249011.1"
                     /translation="MERRREFGRIRSTLAASSHFQALEGADLDRLAELGRLQRLRNAE
                     RAAPAGTRDRFWLILSGAVRVSNETPGSKEYVYAVLGPGSYFGLATAVDDTPFTYDAR
                     AFGATDLAAFRGDSLASMVEERPRVWRHVSELLSRRLRLALAMLRDNSVAPLPERIAR
                     RLLGHALSSDLRGAAQVPVRMTQADLALMLGSSRSRTNAALKTLERSGLVHAGYRGIT
                     ILDVPALKRLAGPGVTAY"
     gene            complement(12700..>13424)
                     /locus_tag="VIG70_00260"
     CDS             complement(12700..>13424)
                     /locus_tag="VIG70_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8249012.1"
                     /translation="LPVALVLRCRWPRGRDWIGVAALGVMFYGAFFVVYAEALVYTTA
                     ARGALAISTLPLLTMIVAALLEKEKLTVRKSVGVLVAMSGVALALAAGLAEAPAQAWR
                     GDLIMTAGMLSMALYNVLSRPFMQRSSPLGYATAGMAFGGGFNALLVLQSGGFAAVAA
                     FGPAQWAGVLYLGVFAGALGFYLWVYALERATPTRVANTITVSPIAAAALGAVLLGEP
                     IGASLLIGIAAVALGIWIASTQ"
BASE COUNT         2276 a         4544 c         4645 g         1959 t
ORIGIN      
        1 ctacaacgtg aacgaggatt tcagccaggc gaacgacctc gcaagcaagc acccggagaa
       61 gctgaaggag ctgcaggcgg tcttcatgaa ggaggcggtg cggaaccacg tgctgccgat
      121 cgacgaccgg cgcgccgagc gcttcaaccc ggcgatcgcc gggcgccccg acctgctgga
      181 tgggcgcaag accctgacgg tctacccggg catgacgggc atgctggaaa acgccttcat
      241 caacgtaaag ggcgtgcgcc acaccatcac cgcagaggta gatctgaagg acgcaagaac
      301 gcagggcgtg atcatcgcgc aggccggcta cttcggcggc tgggtgctgt acatgaagga
      361 cggcaaggtg cgccacgagt acaacttctt cgggctggag cgcaccaaca tcgccagctc
      421 cgaggcgctt gctcccggga agcaaaccat cgtctacgag ttcatcccgg acgaagccaa
      481 ggccgggacc ggcggcaggt cgatcctgcg cgtcggcggt aagcaggtcg ccgaagggaa
      541 gatcccgaag acccagccgt tcgtgttctc cgcggatgaa ggcaccgacg ttgggctcga
      601 cggcgagacc aacgtgtcca ccgactacaa gcagggcaac aacgccttca ccggcaggat
      661 cggcaaagtg acggtcagcg tggaatagcc ggccatgaga gcactcgtcg cattcctgtc
      721 gctgctcacg ctcgcggcga acgtccacgg cgccgatacg ctgccgtcgt ggaatgaagg
      781 cgcgaccaag agcgcgatcg tcgacttcgt cgccagggtg acgaagaaag gcggcaagga
      841 ctacgtcccc cccgcgcagc gcatcgccac cttcgacaac gacggcacgc tctgggcgga
      901 gcagccgctc tatttccagt tcctgttcgc gctcgaacgg gtgaaggcca tcgcgccgca
      961 gcacccggag tggaaggaca aggagccgtt cgcttcgctc ctcaagggcg acctcaaggg
     1021 cgcgctagcc ggcggcgagc atgcgctgct ggaaatcgtc gccgagacgc attccgggat
     1081 gacgaccgag gagttcgacc gcatcgtcgc cgactggctg cgtacggcgc ggcatcccag
     1141 gttcaagcgt ccgtacaacg agctggtcta ccagccgatg ctcgagctgc tgcgctacct
     1201 gcgcgcgaac ggcttcaaga cctacatcgc ctcgggcggc ggcgtggagt tcatgcgcgt
     1261 gttcgccgag cgcgcctacg gcatcccgcc cgagcaagtc atcggcagct cgggcaagac
     1321 gaagttcgag ctgcgcgacg gaaaaccggt gctggtcaag ctgccggggc tcgacttcct
     1381 cgacgacaag gaaggcaagc cgatcaacat ccagaagatc atcggccggc ggccgctcgc
     1441 cgcgttcggc aactccgacg gcgatctgca gatgctgcag tggacctgcg ccggcccggg
     1501 cgcgcgcttc tgcctcttcg tgcatcacac ggacgaggcg cgcgaatggg cctacgaccg
     1561 cgcctcgcac atcggccggt tcgacaaggg cctcgacgag gcgcgcgcca aaggctggac
     1621 ggttgtagat atgaaacgcg actggaaagt cgttttccca tttgagaaga aataggaggc
     1681 aagaatgaag cgagctctcg cattcctcat aacggcgggc gccgcactgc tcttcgccgg
     1741 cgcggccggc gcgcagcagg ccaagcggcc gaacatcgtc gtgatcttcg gcgacgacat
     1801 cgggatgtgg aacgtcggcg cctatacgca cggcatgatg ggccgcacgc cgagcatcga
     1861 cagcatcgcc cgggaaggaa tgctgttcac cgatcactac gcccagccga gctgtaccgc
     1921 gggtcgggcg gcgttcatca ccggccagct cccaatccgc accggaatga cgaccatcgg
     1981 cattcccggc tcgacgcgcg gcatccagaa gaccgacccg acgcttgcga ccgtgctcaa
     2041 gacgcagggc tacgccacgg ggcaattcgg caagaaccac ctcggcgacc gcaacgagtt
     2101 cctgcctacg gtgcatggct tcgacgagtg gttcggcaac ctctaccacc tcaacgcgga
     2161 agaggagccg gagcagctcg actaccccgg ccagaaaaac cccgagtacc tcaagcgcat
     2221 ggggccgcgc ggcgtgctgc acgcctgggc cacggacaag gacgactcga ccaccgaccc
     2281 gaaattcggc cgcgtcggcc ggcagcgggt cgagaacacc gggccgctga cgcgcaagcg
     2341 catggagacc ttcgacgccg aagtgctggg ccataccctc aagtggctgg acggcgtcaa
     2401 ggacaagccg tttttcctct ggttcaacac cacggccatc cacattcatt cgcattcgcc
     2461 gcgcaagtac atccagatgg cggtggacga gggccgtggg gaagaagacg tggtgcgcgc
     2521 caaaatgatc gagcacgacg aacaggtggg cgccatcctc aaaaagctcg cggacctcgg
     2581 cgtcgccgac aacaccatcg tcatctacac caccgacaac ggcttcgagt tcatgctgtg
     2641 gccggacggc ggctatgcgc cgttccgcgg cgagaaaggc agcacctggg aaggcggcgt
     2701 gcgcgtgccg ttcctcgtca agtggcccgg caggatcaag cccggcagcg tgtcgaacgg
     2761 catccagaac cacgaggacg tgttcgccac cctggcggcg gcggcggggt tgcccgacct
     2821 caagaaggag ctgatggccg gcaagaagat gggcgcgacg agctacaagg ttcatctcga
     2881 cggctacaac aacctcgccc actggactgg cgcaagcgag aaatccgcgc gccgcgagat
     2941 cttctactac gacgagaccg acctgatggc cctgcgcgtc gacgcctgga agatgcacat
     3001 cggagcgaag aaggaaggca tctggtggaa cgagaagcac taccccagcg tgccctacct
     3061 gttcaacctg ctgatggatc cgatggaaaa gatggaccag gagtcccacg agtggggcgc
     3121 ggcggcgcgc aggttcttcg cttcaaagat gtgggcgcta aactcgtcgg cgccgttcat
     3181 ccaggcccac ctgaagaccc tgatggacta tccgccgagc cagggcgccg acacgctgag
     3241 catgaagcgg gcgctggacg aggtaatgcg caagctcgaa aagccgcaga ccgcccgata
     3301 gcccgcttcc aatgcgtacg ttgaagaacg ttcttgcggc gctctgcctg attgctggac
     3361 cagggctgct ccagggttgc gagggcatgg aagtcaagcc atcgtcggag ttcggctacc
     3421 agcgaaactg gcacccgcct gcgtgaggtg ataaaaggcg gctaagaggg agcgcatggc
     3481 cgaagaagcc gccgcagggt acctggctgg gcgcatcgac gcggtgcgcg agcacctgtc
     3541 gtcgctcgcc gcagcggcgc cgcggctggg cgccgagttc gggaaggtgg gcgacgagct
     3601 ggcgagggaa tggcagacgc gcgcgccggg ctcgatcctc gccctgatcg ccgcctgcgt
     3661 cctcctcggc tacctggtca gggcgggtta ccactactgg gtgcggggcg ccgaggtccg
     3721 ggacgccacg gtgcgcgagc gcctgcgctc gatcgggctg cgcttcgccc gcgaggtcgg
     3781 cggcgtcgcc gcgttcgcca tcggcagtgc cgcggtagtg ctcattttcg agtggccggc
     3841 gcgcacgcgc gaggcggtgc tcggcttcct cgccgcgttc gtcgtggtgt ggctggtcat
     3901 cgtcgccagc cgcgcgctgc tctcgccgcg cgaagcgcgc ctgcgcatcc tgccggcaag
     3961 cgaccccgag gcggcgttct ggcactcgcg catcgtcctg ttcgcgagct ggttcgcctt
     4021 cggctgggtg atcaagagct ggctgctgct gctcggcatg gacctcccgg cgctgcgcct
     4081 ggtgatctac gcgctcggac tcgggctgct cgcgatcgcg atcgaggcct tctggcgtcg
     4141 ctcgcgcctg gccgccctct atttcgtgct gctctgggtg ctctggtcga tgcgggcgtt
     4201 cggcgcgctg tggctggcgg tcgtcgccgc ggcgcttccg gtagtgatgc ggctcgttca
     4261 gcgcggcgcg gaccacgttc tgcggccgcc ggggacggcc gcggccgaag cccgcggggt
     4321 cgcggcggcg acgatcgggc gcgccctgcg cgcgctggcg atcatcggcg cggcgtacct
     4381 gctggtggac gccttcggcc tggacgtcga cgcgctcgcc gcgtccgatg cgatggggat
     4441 gcggctcgcg cgcggcgcgg tgcacgcgct cgtcatcgtc ctcatcgccg acgtcgcgtg
     4501 gatggtgtgc gcagcgctca tcgaccagaa gctggcttct gttcgaacct ccacgtctcc
     4561 ggaaaacgag catcgcgacg gtcgcctgcg cacgctcctg ccgatcgtgc gcggcacgct
     4621 cgccgtggtg ctgatcgtga tgaccgcgct gatggtgctg tcggcgctcg gcatccaggt
     4681 cgggccgttg cttgcgggcg ccggcgtgct cggcctggcc gtcggcttcg gcgcgcagac
     4741 cctggtgcgc gacatgttca gcaagttctt ctacctgctc gacgacgcgt tccgcatcgg
     4801 cgagtacatc cagagcggaa actacaaggg cacggtcgag catctcggcg cgcgctcgat
     4861 ccgcctgcgc caccaccgcg ggccggtcta taccatcccc tacggcatac tcggcgcggt
     4921 gcagaacatg agccgcgact gggtgatcga caagctgcag atcggcatca cctacgacag
     4981 cgacctggaa aaggcgagga agctgatcaa gaagatcggc caggagctgg cgcaggaccc
     5041 tgcccttgcg ccaaacatcc tcgagacgct gaagatgcag ggcgtagagg acttcgggga
     5101 tttcgcgatc aagatccgca tgaagatgat gaccaagccg ggcgagcagt tcgtaatccg
     5161 ccgcaaggcc tttggcatga tcaagaaggc tttcgacgcg aacggtatca agttcgccta
     5221 tccgacggtg caggtggcgg gcccggccga gggcgccgtc gcggcggcgg cggcccagca
     5281 ggcgctgaag ccggccccct agaggaatat cgtcatgctc cgcattgtca tcgtcgcagc
     5341 gacgctcggt ctcgcgagca cccaggccgc ggcccaggcc tggatcggcc tgctcaagaa
     5401 cacgcccgcc gagcgcttcc aggaggacga cctgcagatg ttcctcgagc acgcgcgcaa
     5461 ggcgctgaac gaggcgcccg acaaccagcc ggtgagctgg gagaacccga agacgcgcgc
     5521 ccgcggcgat gtcaccgtga tgaggagctt cgaatacaag gggctgccgt gcaaggagct
     5581 gcgcgtgcgc aacgaggccc agggccgcaa gggcgacaac accttcaacc tgtgcaacgc
     5641 cgacggcaag tggcgcctgc tcagctcttc acagctcaag agcaaaaaat agctttcgat
     5701 ccgaggtagc cagcgagcgg tgggagtggg gcaaaatgcc tccatcaccc ccgctcgaga
     5761 ctacgtgatt gcttagacgc tgcgtccgcg cggccgccgt tgcctgcgcc gcgctcgcct
     5821 gcgctgccgc gctggcgcag gagcccggct cgaccggccc gaaataccgc ctgacggtgg
     5881 aagccccgac caaggagctg cgcgaaatgc tcagcaaggg gctgcagctc tcgcgctggc
     5941 aggatgaccg cgagatgacg ccggagctcc tgcgccgcct cgccgacgaa ggggtgcgcg
     6001 aggcgcgcga ggcgctcgcg gcgcagggat acttcgcggc gctggtcagc ttctcgctcg
     6061 accgcgaccg cgagccgtgg gcgctgaccc tgcacgtgca gcccggtccg cgcaccgtgg
     6121 tacgcacaac ggagattctt ttcaccggcc ccggcgccac cgacccggag gccgccgggc
     6181 tgttgaagca ggtgcggcgc gactggctgc tgcgcccggg ccagccgttc acccaggagg
     6241 cgtggaacga ggcgaagcgc gaggtcgtaa agagcctctc gtcgtggcgc tatgccgccg
     6301 cgcgcctcgc ttcgagccgt gcgcgagtcg atccggaaac gagcagcgcg gcgctggaag
     6361 tcacgctcga cagcgggccc gcgttcgtct tcggcggcgt cgaggtgcgc gggaccaagc
     6421 gctaccgcga agaggtcgcg gggcacttga gcccggtcga gcccggcgcc acctacgacc
     6481 gccaggtgct cgagctctac acgcggcggc tgctcgagac cggctatttc gccagcgcgc
     6541 gcgtcgacat cctgccggag gggcgcgagg gcggcgccgc gccggtgcgc gcctcggtga
     6601 tcgagggcaa ctcgcagcat ttcgagggcg gggtgtcgta caacaccgac gccgggccgc
     6661 gcctggagct gtcgcaccgc aacgtggatc ttttcaacac ggcgtggcgc ggaaagagcg
     6721 tgctgcggat cgaccagctt acccaggagg cgagctacaa cctcgactcg ccgccgcgcc
     6781 gcggcgccac ctggtggacc ggcaccaccg gcgcgaagca ctcgatcatc caggaggagg
     6841 agaacaccga gctctcgctc ggcgtggcgc acaactgggc cgggtggggc tcccccaccg
     6901 cggttctggt ctcggcggtc gccgaggagc agcgcatcag cggcgaggcg gcggaccacc
     6961 ggcatgcaat ctatttcggc atccgcatcg gcttccgcga caccgacgac atcgtgctgc
     7021 cgcggcgcgg ctacttcggg caggtgaccg cgggcggcgc gcccgacgag ctctcgacgc
     7081 gcggattcca gcgcggcacc gcgcgcatca cattgatgca ttcgctcggg cgcaacgacg
     7141 acctcttgct gcgcgccgaa ggcggcatcg tggtggcgcg ctcgcgccaa ggcatcccgt
     7201 cgacgttcat gttccgcacc ggcggcgacc agacggtgcg cggctacgca ttcgagagct
     7261 tgggtgtgcg ccagggcgac gcggtgctcg gcgggcgcta cctcgccatc ggcagcgtcg
     7321 agtacacgca ctggttcgcg cccgcctggg ggctcgccgc gttcgtcgac ggcggcaacg
     7381 cctgggacac gggcgactac cacccggtgt tcgggtatgg catcggcggg cgcttccgca
     7441 ccccgatcgg cccggtgcgc ctcgacgtcg cctacggcga ggaggaaagg agctggcgat
     7501 tgcacttttc agtgggtttc gtgttctgat gaagcatgag gtggctgaag tggctgcgct
     7561 ggcccctgcg cggcgcggtg gccctggcgc tcgccgccgc ggccgtggtg gtctgggtcc
     7621 tcggcaccga gggcggcctg cgctgggcgc tcggcttcgt cccgcctgag atcgagctgg
     7681 aagcgccgcg cggcgcgctc tggcgcaccg tctccttcga tcgcgtcgcc ttccagggca
     7741 gcgaggcgcg cagcgtagcg ctcgaagtcg acctgctcgc gcttttcagc gacaccatcg
     7801 cggtcgagtt cctgcgcatc gatagcctga ccttgcggcg acccgagggg tcctccgaga
     7861 aatcggagac ccccctgccg tttcgcattc gcgtcgccga tgcgcaggtg aagtccctgg
     7921 tctttgaagg ctacgagatc cacgacctgc aagccgagta ctcgggcagc gcttccggcc
     7981 atgaagcgca ggcgtcgttc tctgctgccg gcgcgcgcgc acgcgcgaag gttgtttttg
     8041 ataaagaaat ttcaattgaa gccgacgtca aggcgatcaa cctcgcggtg atcgatcccg
     8101 gccttcccca gaccgcgctc aacgcaagcc tgaccgcccg ggggtcgact tcggcgctcg
     8161 ccggaacact cacggtcgcc aatccgaacc ccggaccgat cgaccgcgag cggctgccgt
     8221 tttctcgaat cgcggcggcg ttcggaaccg atttcaagct tctgactttg gaaaagctca
     8281 atgctcagct gcacccggcc ggaacggtgc aaggaaaagg cagcgcgagc ctcgagggcg
     8341 cgaagttcga catccgcatc gcagccctcg atctgcgcgc gctctactcg agcctgcgtg
     8401 ccacgcacct cgcgggtgcg ctcgagctcg acctcgcgcg cgagcgccag cgcgtgaagg
     8461 gaacgctgac tcaggaagac atgagcctca ccgcggacgc tgagcgcaag ggcgacgacg
     8521 tcgaagtgcg cgcgctacgc gcgcttgccg gcgacagcga ggcgagcgga agcggccgca
     8581 tccacctcgg cgagccgcta cgcttcgcgg ccgatctcaa gctcacacgc ttcaatcctg
     8641 cgcgcttcgg cgactatccc gaaggcagca tcaatggcac cgtgcagggg aaaggcgatc
     8701 tcaacggccg cgggtcgggg cgctgggaga tcgtcgacag cacgctcttc ggcgagaagc
     8761 tggcgagccg cggcgccgcc accctggtgg gagagcgaat cgtcgccgcg gatgcctggg
     8821 cgactctcgg cgagaaccgc gccacggcaa agggctcgtt cggcgggccg caggatcgcg
     8881 tggcgtggac gctgcacgta ccggatctcg cggcgctcgc tccggacttc ggcggcgaga
     8941 tccgcgccag cggcacagcg accggcacgt ggaagcagcc gagcgtggtg ctggacgccc
     9001 aggcgacgac gctgcgcctc gccgaggcgc tggtcttcga ccgcgccacc gtgaaggctt
     9061 cgggaacgct ggaacgccat gagggcaacc tcacagcgaa gaacgaggaa ttcgatctca
     9121 aagcaaccct gcgcgggggc tggcggcaaa acgcgtggcg cggcgagatc gcatcgctga
     9181 caaacgcggg cgcctatccg ctcgagctga agacgccggc cgcgctcgaa gcgggcgccg
     9241 cgcgcgtggt gctcgggcgc ttcgaggcgg tgcttgccgg cggccgcgcg gcggtcgagt
     9301 cggtgcgctg ggaagaaaag cgcctcacct cgagcggccg catcagcgcc ctgcccgcgc
     9361 aatggatcct caccgcgctc gacgtcgaca aggtgaaggg cgacctcgcg ctcgacggcg
     9421 actgggagct cgcctccacg ccgcggctga acggccgggt cgcggtgcgc cgcgcgggcg
     9481 gcgacctggc catcggggag gtcgcgatgg agctcgccag cgcctccgtg caggcgacgt
     9541 tcactgacga tcgcgtggaa gcgaccgccg aagcggtttc gcggctggtg aaagtccagg
     9601 cgaaaggcac cgcggccggg ctcacgcccg actcggcgct cgcgctctcc gccgagatcg
     9661 aggccgcgga gctgcgcagc ctgaccgagc cgctctggac gcaggcgcgc gtatccggcc
     9721 gcgtgggcgc ctcgctcaag gtggaaggaa cgccacgcgc accggttgtt tccggaaccc
     9781 tgcgcggcga cgcgctcggg gtcgaaatgc cggtgtgggg cgtcgcgctc aaggatggcc
     9841 gcgtacgcgc cgagctcgac gccaaccggc tgctcgtcaa ggaagcgcgc atcgccgccg
     9901 gcgacggcag cttcagcgca agcggcacgc tgccgcttgg gggcgatggc gccgcgtcgc
     9961 tcgattggca ggcgcagcag ttccgcgtgc tctcgaggcc cgaccgccgc ctggtcgtct
    10021 cgggcaaggg cgtcaccacc ttcgacggca agcgcttcgg gctggtgggc gagctgcgcg
    10081 ccgacagcgg gcgtttcgag ctcgccgccg atacgctgcc gacgctcgac gacgacgtcg
    10141 aggtggtcgg ccagaaggcc gacgagatcc gcctcacgac acgcaagcag ggcccgctgc
    10201 cgctcgacct cgacctcaag gtcgacctcg gcaaccggct gaccgtgcgc gccttcggct
    10261 acgacggcgg cgtagcaggc aagctgcgcg tagcgaccaa tccggcgggc gagctgatcg
    10321 cgcagggccg catcgaggcg gtcaaggcgc gcttccgcgc gtacggccag gagctccagg
    10381 tcgatccggg catcgtcatc ttcgacggcc cgttcgatag agcgggcctg gacattaccg
    10441 cctggcgccg gcaccagcag gtcgaggccg gaatcaggct caccggcacg ctgcagaccc
    10501 cgcgcgtcga gctgatctcc aacccgccgg tcgccgaaaa cgagaagctc tcctggctgg
    10561 tgctcgggcg cgcgcctacc gacgcgaacg gcgccgacct ggcggtgctc caggcggcgg
    10621 ggggcgcggt gttcggccgc ggcggcgacg aaccgttcaa ccgcaagttc gcgcagcgct
    10681 tcggtttcga cgagctgacg gtgcgcagct cgagcgagct ggcgagcaac gtggtggcgc
    10741 tcggcaagcg ctactcggac aaggtgtact tcagcttcga gcaggcgatc ggcaccacca
    10801 ccgagtacct ggtgaagctc gactacgcgc tcacgcagcg ggtgtcgcta cgcggacaga
    10861 ccggtactac gagcggcgtg ggccttttct accggtattc ctgggattag cgccgaacgc
    10921 tgacgtagcc tttttcccgc atgcagagct ggaactgctg cgcttcgcgc acctcgcggt
    10981 cctcgcgcat atcgttcgac atcacggatt gcgcgctgaa atcaggggcg cgtgcctgga
    11041 gaggcggcgg ttgcgcgaga cgcgcctgct cccggcactg ctgagtatcc aggctggtgg
    11101 tggactggtc cgcgccggat ttttcccact gcgcgacggg agcggcgcag gccgcgagcg
    11161 cgaaaacggc aagccaaatg ggcgttgatt tcatggccca ttccagcaca ttccccggcc
    11221 ggaatctatc gtccgagctg caccaccggg accttgagaa gcagcgcgac gagctgcgcc
    11281 tggcggcgca cgccggtctt ggagaagatg cgatggagct gcgagcggat cgtcgccacg
    11341 ccgacgccgg catggcgggc gtgctccgcc gcggtgcgcc ccatcaggag gccgatggcg
    11401 agccgcgcct cggcgcgcga cagcccgaac agcgcggaga gcagctcttc gctcggcagc
    11461 gccggcgcct cggtgttgag gatccagacc gcggcgcggc tcgcgccgcc cgcggcgccg
    11521 ccgaagtaca ccggcgccac taccatgcgc agccagcgct ccggcggcgt gccgggctgc
    11581 ggcaggcaca gcccgctcgg ctgcagcccg tcgcaggcga ggcggatcgc ctgggcgagc
    11641 gcgtcgcgca ccttggctac cttggccgac agcgtgccgg ccgtgagcgt cagcccgccg
    11701 tgcccgcgcg cgagcagcgt cgaggcgacg caattgcgat actccaccct gccctcgtcg
    11761 tcggcgatga gcagcgcgca gtccagcatg tcgagcacgc gttctagcat taccggcagg
    11821 gctttgctgt tatcgtattg cccggatgcc gggcggtaag gggcgccaaa catggcatga
    11881 tctgatgact ggttcatcca tcagatgatg gaggcgcgac gcgactcgcg ccgcgccgat
    11941 tgcgccgccg ggaacagtcg gcggaaaaat gggcgcggaa tgttccgcgg ggaacgattg
    12001 ccatggagag gcgtcgcgag ttcgggcgta ttcgcagcac ccttgccgcg agctcgcatt
    12061 tccaggcgct ggagggcgcg gatctggacc ggctggccga gctcggccgg ctgcagcgtc
    12121 tgcgcaacgc cgagcgcgcg gcaccggccg gcacgcgcga ccgcttctgg ctgatcctga
    12181 gcggcgcggt gcgggtgagc aacgagaccc ccggctcgaa ggaatacgtg tatgcggtgc
    12241 tcgggccggg aagctacttc ggcctcgcca ccgcggtgga cgacacgccg ttcacctacg
    12301 acgcgcgcgc tttcggcgcg accgacctgg cggcgttccg cggcgactcc ctggcgtcca
    12361 tggtcgagga gcggccgcgc gtgtggcgcc acgtctcgga gctgctttcc cggcggctgc
    12421 ggctcgcgct cgccatgctg cgcgacaaca gcgtcgctcc gcttcccgag cgcatcgcgc
    12481 ggcgcctgct cggccacgct ctgagcagcg acctgcgcgg cgccgcgcag gtcccggttc
    12541 gcatgaccca ggccgacctt gcgctgatgc tcggctcgag ccgctcgcgc accaacgcgg
    12601 cgctcaagac gctcgagcgc agcggcctgg tccacgccgg ctaccgcggc atcacgatcc
    12661 tcgacgttcc ggccctgaaa aggctcgccg gccccggggt cactgcgtac tagcgatcca
    12721 gatccccagc gcgaccgcgg cgatcccgat caggaggctc gcgccgatcg gctcgccgag
    12781 cagcacggcg ccgagagccg cagcggcgat gggactcacg gtgatcgtgt tcgcgacccg
    12841 cgtcggcgtc gcgcgctcga gcgcgtaaac ccagaggtag aagccgagcg cgccggcgaa
    12901 caccccgagg tacagaaccc ccgcccattg cgcgggcccg aacgccgcga ccgccgcgaa
    12961 gccgccgctc tgcaagacca gaagcgcatt gaaaccgccg ccaaaggcca tccccgcggt
    13021 cgcatagccg agcgggctcg agcgttgcat gaaagggcgc gacagcacgt tgtagagcgc
    13081 catcgagagc atgcccgcgg tcatgatgag gtcgccgcgc caggcctgcg caggcgcctc
    13141 ggccaggccg gctgcgagcg cgagcgcaac cccgctcata gcgacaagca cgccgacgct
    13201 cttccgcacc gttaattttt ctttttccaa aagcgcggcc acgatcatgg tcagcaaggg
    13261 cagcgtggaa atcgcgagcg cgccgcgcgc cgcggtggtg tacacgagcg cctcggcgta
    13321 cacgacgaaa aaagcgccgt agaacatgac gccgagcgct gcgacgccga tccagtcgcg
    13381 tccgcgcggc cagcggcagc gcagaaccag tgccaccggc agca
//
LOCUS       DATBSZ010000005        43523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_888649, whole genome shotgun sequence.
ACCESSION   DATBSZ010000005 DATBSZ010000000
VERSION     DATBSZ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 43523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43523
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888649"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /gene="metW"
                     /locus_tag="VIG70_00265"
     CDS             complement(<1..521)
                     /gene="metW"
                     /locus_tag="VIG70_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEY8249013.1"
                     /translation="MGTARRARAGPRLGDGALLRSLWLEREAPGYGVEIEDEAVLACV
                     ANDVNVLQVDLESGLSLFADGSFDCVILSETLQTIHRTEFLLREMLRVGREGIVSFPN
                     FGHWGARLQIALGKMPVSETLPYPWYETPNIHHCTITDFEDLCRRIGIRIRERLVLHA
                     GKPVTVMPNLLGSL"
     gene            complement(547..1683)
                     /locus_tag="VIG70_00270"
     CDS             complement(547..1683)
                     /locus_tag="VIG70_00270"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY8249014.1"
                     /translation="MNAPHDPSSVGAATAQKAHFSEPLHLRGGAELPSYEIAYETYGT
                     LNAAKSNAVLVCHALNASHHVAGFYEKEKDNVGWWDNLVGPGKPLDTNRFFVVGSNYI
                     GSCFGSTGPASVNPATGKPWGADFPVVTVEDWVEAQRRLADRLGIERFAAVIGGSLGA
                     MQALQWTLSYPERIRHSIVIAAAAKLTSQNIAFNEVARQAIMTDPDFHGGHYYDKGVV
                     PVRGLRIARMIGHITYISGEAMAEKFGRVLRRGQPGFDFDIDFEVESYLRYQGDKFSS
                     YFDANTYLRITKALDYFDPAADFGGDLTKALARAKAAFLVVSFKSDWRFTPARSREMV
                     RALLDNRRIVSYLELDAPGGHDAFLLEDPRYHAALRSYFANIDL"
     gene            1796..2722
                     /locus_tag="VIG70_00275"
     CDS             1796..2722
                     /locus_tag="VIG70_00275"
                     /inference="COORDINATES: protein motif:HMM:NF040426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6454 family protein"
                     /protein_id="HEY8249015.1"
                     /translation="MRPILAIAALLASNAVTASELVAERVQKLTRAVRWQPVAEIPVG
                     FKTHHPQGMAKIGDTFFVSSVEITVPTKRFPELKDGYDRDTGEGVGHLFRIDAKGNLL
                     GDLKLGEGSIYHPGGIDYDGRHLWVAVAEYRPNSRSIIYRVDPQTMQATEMLRFADHI
                     GGIVHNTDDRTLHGVSWGSRRLYKWTLGRDGKVTNAGAPPEKLRRPNRSHYIDYQDCH
                     YVGGRRMLCSGLNNYRMKADGPAFPLGGLEIVDLKTDQAVYQVPVELWSPSGKPMTQN
                     PFWVEATDNGLRAYFMPDDDKSTLFVFEAAVK"
     gene            complement(2741..3940)
                     /locus_tag="VIG70_00280"
     CDS             complement(2741..3940)
                     /locus_tag="VIG70_00280"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8249016.1"
                     /translation="MSGPLHGVRVLDLTTVVMGPYATQILGDFGADVIKVEPLEGDVI
                     RQAWPFRNPGMGAIFLNVNRNKRSVALDLKQAAAREACLQLAKRSDVLVYNIRPQAMT
                     RLKLGYEEVKKVNPKIIYVGCFGYSQRGPYAAKAAYDDMIQGASGLPWLLQKQGAPEP
                     RYAPMIVADRSVGQQVASAVSAALYHREKTGKGQRVDVPMWEHLLQIVLSDHLGGYTF
                     EPQKGEPGYLRILTPDRRPYQTQDGYVCALIYNDKQWKSFFDIIGRPEMLKRPEFATH
                     DARSQNYQAAYAMIAEEMKQRPTAFWLEALERGDIPVQRMNSLDDIVADPHLNAIGYL
                     QLVDHPSEGRIRMLAVPSEWSESKPEYRRHAPRLGEHTREVLAEAGLSNDKIQALIDT
                     GAARAAD"
     gene            complement(3937..4248)
                     /locus_tag="VIG70_00285"
     CDS             complement(3937..4248)
                     /locus_tag="VIG70_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249017.1"
                     /translation="MKRRLHELAHARAGDKGDTLTLALFPYREEDYELLRREVTAQAV
                     KRHLAHLVGGEVRRFEVPGLHALQFVCERALDSGVTTSLAIDPHGKTLSYALLEMVID
                     A"
     gene            complement(4229..5584)
                     /locus_tag="VIG70_00290"
     CDS             complement(4229..5584)
                     /locus_tag="VIG70_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEY8249018.1"
                     /translation="MPESSLNSIRIGAGSGFQGDRLEPAVILASQGELDYLALECLAE
                     RTIALAQLRKLADPAAGFDPLLEARMRALLPLAVRHRFRIVTNMGAANPLGAGRRVMA
                     LSREFGLKVRIAVLSGDDVLSLLEPGMRTIEARGAALSEYGALVSANAYFGAEEMLPA
                     LASGAQVVITGRVADPSLFVAPLAHEFKWRLDDWPRLGRATAIGHLLECAGQVTGGYF
                     AEPGLKDVPNLAQLGFPWCDVDAEGNGVLGKVAGTGGAITLRTAKEQLLYEVTDPGGY
                     ITPDVVADFSTVSLQESAPDRVAVRGAGGRPRTPTYKVSVGYRAGFVGEGEISYAGAK
                     AVERARLAGEIVRERLRDDVTDLRVDLIGMDSAHRTDFSHSLPYEVRLRVAARTATRE
                     LAERVGAEVEALWINGPAGGGGVRRHAQERIGVVSLLLERARVRPKVTLLDEDREAQA
                     A"
     gene            complement(5562..6509)
                     /locus_tag="VIG70_00295"
     CDS             complement(5562..6509)
                     /locus_tag="VIG70_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249019.1"
                     /translation="MKWLSSLLVALAIPLAHAQTYPSKPVKLVVPFPAGSATDQVARV
                     VGQQLQESLGQPFVVENKPGAQGSIASAEVARAAPDGYTLMVGTNTPLAANPSLFKKL
                     NYDPVKDFAPVARLGTISFMIMVRPDFPAKNLKELLAHAKANPGKLSAGYGSAGSQVS
                     QAMLRSMGKIDFIDVPYKGLPQAITDVLGGQISFTFADLANALAQIKGGKLRGIAVTS
                     QKRSALAPDVPAIAEELAGYELIAWFALVAPAGTPPAVVARLHDITAKSLAKPEVIAR
                     FASLGTDVAPMNPEQLGGFIRSEIAKFARLAKDAGIQPE"
     gene            complement(6521..8254)
                     /gene="ptsP"
                     /locus_tag="VIG70_00300"
     CDS             complement(6521..8254)
                     /gene="ptsP"
                     /locus_tag="VIG70_00300"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY8249020.1"
                     /translation="MNFVLHGYPVSSGITVGNAHLVSTARLEVAHYEVAPEALDAEVM
                     RFDRAMLAAQEELAALKAHIPSGSPAEFEAFLDLHRMILNDSALALAPRETIRKRRAN
                     AEWALVQHMERLVERFEEIDDPYLRERKADVQQAVERVLKALMGGQTLSPPAISEEEK
                     LIVVAHDLSPADMILFKRHRFGGFVTDVGGVTSHTAIVARSLGIPAVVGLHHARETIA
                     EGDTLIVDGTQGLLIVNPDPLVLAEYRGRQADLKTEKKRLRRLKSTPAATVDGTPIEL
                     YANIELPQDMPEVLEAGAEGVGLFRTEFMFLNRKDLPSEEEQLEAYREVAKAMQGRPV
                     VLRTLDLGADKALNGSGEGSHTMPNPAMGLRAIRLCLAEPQMFLTQLRAILRASHYGK
                     VRMLLPMLAHAHEIDQTLALIAQAKAQLDERRQPYDQDIPIGGMIEIPAAALALPMFM
                     RRLDFLSIGTNDLIQYTLAIDRTDDSVAHLYDPLHPAVLTLVAGTIQTATRGGVPVAV
                     CGEMAGDLQLTRLLLGLGLRNFSMHPSQLLAIKERILRTNLAEVQALAQRVLRASDPV
                     KTRDLLAKLNA"
     gene            complement(8251..8520)
                     /locus_tag="VIG70_00305"
     CDS             complement(8251..8520)
                     /locus_tag="VIG70_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY8249021.1"
                     /translation="MPSVSATIVNRLGLHARAAAKLTHLAGTYPCEIWISRSGRRVNA
                     KSIMGVMMLAAGQGTSVLIEAEGADAEQAIAAITKLIADKFGEGE"
     gene            complement(8513..8917)
                     /locus_tag="VIG70_00310"
     CDS             complement(8513..8917)
                     /locus_tag="VIG70_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249022.1"
                     /translation="MIGVLIVSHGDIGTSLLTSASQILGDEQKQVATLSVWRADDVDD
                     LVLRARELMESLDSGDGVLVLTDIFGATPGNVVSRLLEDGRIEGVSGASLPMLLRVLT
                     GRNGSLSAAVKRALSGGAEGVVHMNNDRCCDA"
     gene            complement(8914..9855)
                     /gene="gshB"
                     /locus_tag="VIG70_00315"
     CDS             complement(8914..9855)
                     /gene="gshB"
                     /locus_tag="VIG70_00315"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY8249023.1"
                     /translation="MEFLFVIDPPDSLKAYKDTTVAMMRAAQKRGHAIWVCEQASLHW
                     LQGRVGAAALRISLADNDEDWFRPHETAQRPLADFSAVLMRKDPPFDLEYVTSTWLLS
                     QAVREGAHVYNAPDAIRDHNEKLAIAEFARFAAPTLVSREPERIQAFVDSERDIVVKR
                     LDVMGGENVFRVRADDPNRNVIVETISQGARRSVMAQRYIPEISQGDKRVLLIDGAVV
                     PHCLARIPKAGDSRGNLAAGARGVAQPITARHREIAAALGPVLAKRGLLVVGLDVIGD
                     WLTEVNVTSPTCFREIQDQTGFDVSGMFLDALEKKLR"
     gene            complement(9862..10626)
                     /locus_tag="VIG70_00320"
     CDS             complement(9862..10626)
                     /locus_tag="VIG70_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8249024.1"
                     /translation="MEAHEHESFIKTPQQLIVVILLAFLVPIIGILLLVQLVLSRPSA
                     DPQALTAESIAARIQPVARFEIGAPAAAPGARSGEAIVQATCSACHQAGVAKAPKIGD
                     KNDWAPHLKHGLNAMVESVIKGKGAMPPKAGDPSLTDDEIRRAVVHMANLAGGSFKAP
                     AAPKEKPPPAHAGHAADGKAVYDKICFACHQQSVAGSPKLGDKEAWAPRIKTGTGAMV
                     QSVIKGKGAMPPKAGNPALSDAEIRAAVEFMVSQSK"
     gene            10727..10803
                     /locus_tag="VIG70_00325"
     tRNA            10727..10803
                     /locus_tag="VIG70_00325"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10761..10763,aa:Arg,seq:ccg)
     gene            complement(10822..11658)
                     /locus_tag="VIG70_00330"
     CDS             complement(10822..11658)
                     /locus_tag="VIG70_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8249025.1"
                     /translation="MPTPFTRVVFVLLVTLTAGCTNTFFQPQRVLVDTPASAGLEYEV
                     LPLRAADGVELFTWFLPARGTPQATVLFLHGNAENISTHYAAVAWMPAEGFNVLVLDY
                     RGYGRSSGAPSLAGVQLDIDAALRALLERRDVDPRRIVLFGQSLGGALAIHYAARGAH
                     RSSVRAVIADSAFADYRLIVTEKMAGFFLTWPFQWLPALTVDNDYSPRSSVGAVSPVA
                     LLLIHGEGDGIVPPHHSRQLYELAGQPKELWIVPAAGHIQSLQDWALRSRLTDFVRRA
                     VR"
     gene            11753..12244
                     /locus_tag="VIG70_00335"
     CDS             11753..12244
                     /locus_tag="VIG70_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HEY8249026.1"
                     /translation="MKKLVAAAAIAALFPAVALAQQNNVGSCGWGSKLMDGQSGVFPQ
                     ALAGITNGTFGNQTFGVSTGTSGCTQDGTVRSTWKTAMFIDGNKERLARDMSVGGGEA
                     LDSLAHILGVDQTDRAAFNRLARDNMARIFPSENANTEQVVSALRAVLMTDAKLARYT
                     VAL"
     gene            12416..14176
                     /locus_tag="VIG70_00340"
     CDS             12416..14176
                     /locus_tag="VIG70_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEY8249027.1"
                     /translation="MHYVPNLIAPGVHGQVDSRAFYNAPDGKNDPGAELEATLLSFFS
                     PAPDSDERQHPQCRFPARYGWLAAELGFDARRMPHPECRRYREWRTALDAKQLTLVFA
                     SAYLNNPGSMYGHTLLRVDAADQDERTRLLAYTISFAANTNETNGLIYAVKGLFGGYA
                     GVISMLPYHAKVREYGDLENRDLWEYELDFTAEEVERVLRHAWELGPSYFDYYFFDEN
                     CSYHLLGLLQVARPDLELTAPFPLWALPIDTVRVLTERPGLVRRITYRPANSTILGAR
                     LAGMRPEERRFARDLGLGTLSASDAALRALPPERAAAVIEAGYDYLIYRRTTGVSEVP
                     DGGALAHDLLVARGAVDAPSQAPRIEPGVRPDQGHRTSRLAAGVGRREREDFVELGVR
                     ATYHDIVDDDAGYIGGAQIEFFKLRARRYEHGDARIESFIPVEIFSLSPRDEFFQPRS
                     ARLAFGWQRTFTRSGAEPLALGAEGGFGGAWKPRTGARFYATVEAAVRAHDAFEEGYT
                     LGAGARLGALVDPTARWRIHAYAQQLGSLAGERHDPGALVLEQRFTLTRDMALRFDLS
                     RQRESGRTFDTALLSLQLYF"
     gene            14236..15201
                     /locus_tag="VIG70_00345"
     CDS             14236..15201
                     /locus_tag="VIG70_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
                     /protein_id="HEY8249028.1"
                     /translation="MIRVFGAAALFTLATSAAAQQYPNRNISMIVPYAAGGPTDTVAR
                     VTAQAMGKPLGQTIIVENRPSAGGILGPEQVKNSKPDGYTILIHHIGMATTPALYRNL
                     RFKPLEDFEPIGLINDVPMTIIGKPQLPAKDFKEFLAYIKKNKDKVSYANAGIGAASH
                     LCGMLFMSAIETDFLTVPYKGTAPAMNDLLGGQVDFMCDQTTNTTSQIKSGKVKAFAV
                     TSAKRVPSLPDIPTLAEVGVKGAEVGIWHGLYAPKGTPKPVLDKLVGALQDALKDEGV
                     QKRFSDLGATTYPVSMATPAALQKHLKAEMDKWGPLIKKAGVYAD"
     gene            complement(15258..15926)
                     /locus_tag="VIG70_00350"
     CDS             complement(15258..15926)
                     /locus_tag="VIG70_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY8249029.1"
                     /translation="MITVHHLNNSRSQRVLWLLEELGLEYEVKRYQRDRQTMLAPPEL
                     RAVHPLGKSPVIVDGGVALAESGAIVEHLADRYGAGKLAPAFGSPERVRYLYWLHFAE
                     GTAQPPLLLKLLFDRIKTKSPFFVRPVARAIADKALTTFVLPNLGRNLDFMESELGKS
                     EWFAGAQFSAADIQMSFPLEASRARGGLDEKRPRLMNFLEKIQARPAYRRAIERGGEY
                     ALMR"
     gene            complement(15968..16843)
                     /locus_tag="VIG70_00355"
     CDS             complement(15968..16843)
                     /locus_tag="VIG70_00355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8249030.1"
                     /translation="MEDSRRDLKNDDLNSLVPDVQVDRRGFVAACIAAGFAVTAEPIL
                     AQAIKTSMDGLDGGDVKIGEIPAYYAVPKGKGKRPVVLVIPEIWGLHEYQKDICRRLA
                     KAGYFGVSLDPTFRIGDLKTVTDIKQSIAMANQLTDQQAFADLDAVVAWAEKHPRANA
                     GKLGITGMCRGGRMVWMYTAHNKKIDAGVAWYGSLMPIPPAMPTGPLDVTDKVDRPVL
                     GLYGSADQGIPMDHVERLRAGLLAFGNDKKSTIHVYEGMPHAFHADYRPTYRKEAAED
                     GWKRMLAWFKKNGVA"
     gene            complement(16943..17497)
                     /locus_tag="VIG70_00360"
     CDS             complement(16943..17497)
                     /locus_tag="VIG70_00360"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY8249031.1"
                     /translation="MKRCSWPTTELDITYHDREWGVPVHDERVLFEFLILEGAQAGLS
                     WSTILKKRENYQRAFARFDPKKVARFDAARKRLLMKDEGIVRNRLKIESTVSNARAFL
                     AVQKEFGSFDRYIWQFVGGQPIQNSRKGRDIPARTAESDAMSKDLKKRGFRFVGSTIC
                     YAFMQATGMVNDHLTACFRYRELK"
     gene            complement(17494..17802)
                     /locus_tag="VIG70_00365"
     CDS             complement(17494..17802)
                     /locus_tag="VIG70_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEY8249032.1"
                     /translation="MLELRPTCEHCNKALPPNSPEARICSYECTFCGACVEKVLGNVC
                     PNCGGGFVPRPVRPSKNWKGDNFLGKDPASTKVKHRPVDPAAHAKFSTPIKGIPPEKR
                     "
     gene            complement(18366..19637)
                     /locus_tag="VIG70_00370"
     CDS             complement(18366..19637)
                     /locus_tag="VIG70_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8249033.1"
                     /translation="MIYSFGNCEVDVDRRELRRGGAAVHVEPQVFDLLVHLVQNPGRV
                     LTKDKLIEAVWNGRIISDATLTSRISALRRAVHDGAEQQRTIRTIPRCGYRFDGVVRE
                     REGPSNRLAASPIVIQAGNMEADVQQQITFCRTADGVHLAVATAGSGPTLVKTANWLN
                     HLEYDWRSPVWAPLFRRLSAQCQLVRYDERGNGLSDWDVQFSFEAFVQDLETVVDAAG
                     LGRFALLGISQGASIGIAYAVRHPERVSQLILSGGFVQGWRKRGSSADIARAEASITL
                     IREGWGQDNPAARQMFTSLIVPDATLEEMRWLNDLERVSTTPENAVRLLYAVGEIDIV
                     DLLPQVSVPTLILHSRGDARVPFEQGLLLARTIPNARFVALDSRNHLILSHEPAWQRY
                     VNEVCCFLKGADTLSGSPAVSRGLLRSRLDG"
     gene            19783..20625
                     /locus_tag="VIG70_00375"
     CDS             19783..20625
                     /locus_tag="VIG70_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8249034.1"
                     /translation="MSHPNPLRRDVLRLTLSAAAALAAVPMSPALAQSTRKATFVLVH
                     PAWHGAWYWKKVTPLLRAKGYEVYTPTLTGLGELAHLARPEVGLRTHIEDVVSVLEYG
                     DLGDVILVGNSSGGMVITGVAERAPARIAQVIYLDAFVPGDGQSLVDLLPPERKHAFE
                     EFAKSEGKGWLVPRFAPLPWEHIVRKLWGVNDDADVRWMLARLVPTPLGHFTEPVQRT
                     NPASDKLVRTYVRCLQFKHPGFDQHAAMAQRAPNWRYREMATPHLPPITHPRELTDLL
                     VQLT"
     gene            complement(20608..22029)
                     /locus_tag="VIG70_00380"
     CDS             complement(20608..22029)
                     /locus_tag="VIG70_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8249035.1"
                     /translation="MLSEICFTPARTLARLIRARKLSATEVVRAFIAQIERVNPKVNA
                     IVTFLPDQALNAAKSQDRKKAFAGPFAGLPIAIKDVIATKGVRTTWGSLVYKDHVPTE
                     DHIMAERLKAAGAILIGKTNTPEFGAGSQTFNKVFGATRNPYDLSKTCGGSSGGAAVA
                     VACGMVPFADGGDFAASLRNPGNYCNVIGFRPSTGRVPAYPHPYPWSNLSELGPMART
                     VSDAAFLLSVQAGPDRRAPTSIMEPGSIFNKPLKRSFKKARVAWSRDLGGLPVEPAVT
                     KVLESQRKTFKDLGCIVEDAEPDFSGATEAFESLRALTWLHRGGALLKEHRDKLKDTV
                     IWNTEEGFKRSAADIAKAESQRTALYHRMRVFLEKYDFFVCPVNQLPPFSVDQPYPTE
                     IAGVKMTNYLDWMKSCYYITLTTHPAISVPAGFTDDGLPVGIQIVGRYRDDFGVLQFA
                     HAFEQATQVWKRRPPLALLGELH"
     gene            complement(22023..23000)
                     /locus_tag="VIG70_00385"
     CDS             complement(22023..23000)
                     /locus_tag="VIG70_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8249036.1"
                     /translation="MRTALIIAASLALAACGGMPLDPQAPKVKITPLGSHDGEFCPLD
                     RALIFEDPDGTRILYDAGRTVRGPNDPRLGRIDAILVSHVHGDHLGDLIQPSANAGAC
                     PKPDFSVSVTPNSNSVNISLAKQAPVIVGGEMGSFLSAKVKSLGGDPAKLVRLVRFGA
                     QTMVGKVMVASVPAVHSNGLPPAFLPPEQARMFEAAGLTAYVGPPGGFVLRFSNGLIA
                     YLSGDTGITAEQDLVVRGHYKANLVVMNIGGFPFTTGPTEAAYVVNDLVRPSSVIASH
                     ANEPATVGGKLSPTSRTAAFQKAAKMPVHVPLSGRTMEFDGSGRCTAGC"
     gene            complement(23027..24475)
                     /gene="rng"
                     /locus_tag="VIG70_00390"
     CDS             complement(23027..24475)
                     /gene="rng"
                     /locus_tag="VIG70_00390"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEY8249037.1"
                     /translation="MEEILINVTPQETRVALTATGVVQELLIERAASRGLVGNIYVGR
                     VARVLPGMQSAFVEVGLERAAFLHVADIWDQRDRPIEKTLSEGQPILVQVVKDPIGTK
                     GARLSTQVSIAGRLLVYLPQDPHIGISQKIEDENGRAALRERLKDLLPADEKGGFIVR
                     TLAETASEQELRADLSYLRELWKAIQQRSGGPNAGATPPLLVYQDLSLAQRVLRDMVS
                     AETARVRVDSRENFQKLAAFAERYMPQVRSRLEHYTGERPLFDLHNVEPEIEKALSRR
                     VELKSGGYLMIDQTEALTTIDVNTGGFVGSRNFDDTVFKTNLEAAQAIARQLRLRNLG
                     GIIILDFIDMESPEHRAAVLDELRRAVARDRTRITLNGFTALGLVEMTRKRTRESLAH
                     VLCEPCPTCAGRGEVRTAHTVCYDILREILREARAFNAREYRVIAGQAVIDLLLEDES
                     TSLGMLSDFIGKPISVQVESGYSQEQFDIVLL"
     gene            complement(24456..25091)
                     /locus_tag="VIG70_00395"
     CDS             complement(24456..25091)
                     /locus_tag="VIG70_00395"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY8249038.1"
                     /translation="MIPIPERGIYLASRSPRRRELLSQIGVRYHLLLFRERPRGGSSE
                     PDIDEAPLAGEPPAAYVERMARAKADAGWSRMLQRNLPPAPVLAADTTVSFQERILGK
                     PRDRAEAAEMLAALSGQRHEVLTGVALKNYDQVEFALSSSEVVLKKLSDDDIAHYAAS
                     GEGDDKAGAYAIQGRAGRFVMELRGSYSGVMGLPLYETSLLLEKLRGRNPY"
     gene            complement(25088..25486)
                     /locus_tag="VIG70_00400"
     CDS             complement(25088..25486)
                     /locus_tag="VIG70_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY8249039.1"
                     /translation="MPRLHVVAVAAKLPGWAEAACVEYLKRMPRGYEVSRIDAKPGKW
                     SSFVKGRLVALDERGKDMTTAQFAPLLKAETTFVIGGPDGLEESVKKDAALLLRLSAL
                     TLPHALAQVVLLEQIYRAATMLTGHPYHRA"
     gene            complement(25452..25844)
                     /gene="rsfS"
                     /locus_tag="VIG70_00405"
     CDS             complement(25452..25844)
                     /gene="rsfS"
                     /locus_tag="VIG70_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010114519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY8249040.1"
                     /translation="MDIRVKQRVVVEALEDVKGHDIVVFNTARLPSMFERVVIASGDS
                     NRQVKALADRVQERVRELGARVYGVEGESSGEWVLVDLGDVVVHIMHPTVRDFYNLEE
                     IWGGKPVKLKGPKKAKARAKAPRRRRSR"
     gene            complement(25844..26386)
                     /gene="nadD"
                     /locus_tag="VIG70_00410"
     CDS             complement(25844..26386)
                     /gene="nadD"
                     /locus_tag="VIG70_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEY8249041.1"
                     /translation="MGGTFDPVHNAHLAMARAALDELELEKLLFMPTGAPRYRTPAVA
                     SGEHRIAMLRLALADNKSCEIDARELAPGASGYTIDTLKELRLELGAATELWLLMGAD
                     QYAKFEAWHRPDDIRKLARIAVFERPGTSLDKRVAVISMKPMPVSASEIRARAGRGED
                     LSALVPPAVADYVRRHGLYA"
     gene            complement(26398..27681)
                     /locus_tag="VIG70_00415"
     CDS             complement(26398..27681)
                     /locus_tag="VIG70_00415"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY8249042.1"
                     /translation="MQLKRNKHAQGDLHSAMQEMGRAARAAARELARAETGAKNRALQ
                     AVAQEIRSRSKDLSKANALDVAKAKKDGCDAAFVDRLTLTPALAEQMAAGVEQVASLP
                     DPVGAISERVKRPTGIEVAKMRVPIGVIGIIYESRPNVTADAAALCVKAGNACILRGG
                     SEAIESNRAIAACVRAGLKAAQLPEAAVQLVGTTDRAAVGELITMREHVDLIVPRGGK
                     ELIERLARESRIPMIKHLDGVCHVYIDDSADPEMAVRIADNAKTQRYGTCNTMETLLV
                     SSGIAQRVLPRISGIFKSKGVEMRADAAALEFVDGKKATDSDYYTEWLAPVVSIRVVD
                     GLDEAIAHIAKYGSQHTDAIVAQDRARAERFLREVDSSSVMWNASTRFADGYEYGLGA
                     EIGISTDKLHARGPVGLEGLTTQKYVVLGHGEIRN"
     gene            27694..29112
                     /locus_tag="VIG70_00420"
     CDS             27694..29112
                     /locus_tag="VIG70_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8249043.1"
                     /translation="MVQDAVIVGGGLAGLACAVALGDAGVRVTLLEREKRLGGRARSW
                     TDAASGDPIDLGPHVVHSEYRNMLAFLERLGTRDLICWQPGKLITFSSGMVLRHRRLP
                     APFSLLPDLVRGSGLSARDLWSNNRVTWRAMRFDEADVPELDRVSALEYLRALGVSEP
                     MIEWFWSFAALSVMNVPLERCSAAALLRVHSQLIGHRRLHFGFPAVALSELFAAQAAN
                     AIERAGGRVLLEAEVAEINDGVVLRDGARIPADHVVAAVPPQDLKKLGLAAPAVEPSP
                     YISSYLWFDRKLTSERFWALLGALKHLNTDFYDLSNIRRGWSARPSVIASNIIYSHRA
                     GTMSDAEIVAATQREIAQFVPEAARSRVVHATVNRIAMAIPCPLPGSESERPAASHCA
                     GISLAGDWTRTGLPCSMEGAVRSGWLAAEEVLRAAGRPRALALAPRPADGLVGLVQRL
                     RSSAARARAWRTPPTARPRPHP"
     gene            complement(29042..30004)
                     /gene="holA"
                     /locus_tag="VIG70_00425"
     CDS             complement(29042..30004)
                     /gene="holA"
                     /locus_tag="VIG70_00425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY8249044.1"
                     /translation="MPRLRTQEQLPAHLAKNLGAAYAILGDEALLVLEAADAVRVAAR
                     KNGFTEREVYEPGRSFDWSELRHAGATLSLFGTKKIVELRLASGKVNAQAAEAIVAWC
                     ERTNPETLLLLTMPRPEGGGWWDAPWFLALNKHGVIVEVQPVTRAALPAWLEKRLALQ
                     KQSASREVLEYLADRVEGNLLAAHQEVQKLALLAPEGELTLETVRGAVADVARFDTHV
                     AAEALLGGDTARYLRILDGLRGEGEAPTYLLFTISSALFALAGGERTSKAMQAVLDKA
                     RRYPPAAIEQAISRAAAIDRAIKGVGVGEPWEEFAKLGLELHST"
     gene            complement(30019..30504)
                     /gene="lptE"
                     /locus_tag="VIG70_00430"
     CDS             complement(30019..30504)
                     /gene="lptE"
                     /locus_tag="VIG70_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY8249045.1"
                     /translation="MSSRRALALALALLLAGCGFRLRGTAEVPFETLYVPSATSGIAL
                     DLKRTIQAGTKARVVDDPGKADAVMQFTEETRQKEILSLTGTGRVREFQLRYRVGFRV
                     HDGKGAEYVPPNTIQLTRDVTFNDAEILAKEQEEQLLFRDMQVDMVQQIMRRLAAAKR
                     P"
     gene            complement(30494..33049)
                     /gene="leuS"
                     /locus_tag="VIG70_00435"
     CDS             complement(30494..33049)
                     /gene="leuS"
                     /locus_tag="VIG70_00435"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY8249046.1"
                     /translation="MQDVYDPAAVEREAQDFWASRDSFRSLEDPAKPKFYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLTRYYRMRGCNVLQPMGWDAFGLPAENAAMANKVPPAKWTY
                     SNIATMKAQLKSLGFALDWSRELATCRPDYYKWNQWLFTRLFRKGLAYKKTGVVNWDP
                     VDQTVLANEQVIEGRGWRTGAPVEKREIPMYFLRITAYAEELLAALDDLPGWPEQVKL
                     MQKNWIGKSEGVRIGFPYQLNNQPQTLWVFTTRADTLMGATFMAVAAEHPIAVFASQK
                     DSKVRAFVDECKRGPAMEAELAQIEKKGMPTGLFVTHPLSGEKLPVWVANYVLMGYGE
                     GAVMGVPAHDERDYEFALKHKLPIKPVIRHPLGDTVPAPWKPEYAEYGTCINSGKYDG
                     LSYREATDAIAADLQRKSLGEKQIQWRLRDWGISRQRYWGTPIPIVHCPSCGDVPVPD
                     EQLPVRLPEDVVPDGTGNPLNKLASFVDTKCPSCGRPAKRETDTMDTFVDSSWYFVRF
                     CSPEVAGAMADERADYWMPVDQYIGGIEHAILHLLYSRFFQRAMQKETGLMRGVDEPF
                     TNLLTQGMVLAESYYTDTSGRREWINPADVEVVRDAKGTIVSARAKDGRAVVYDGIGT
                     MSKSKNNGVDPQALIDKFGADTARFYTMFTSPPTSTLEWSDASVEGSYNFLRKVWGFA
                     FKFSRATCAESIERKIRYQVHLELKKANDDIVRKKFNTVASACMKILNAIEALPEWDA
                     TSKEGLSILLRLLSPIAPHIAHHLWRELKFGEDIMEAPWPEPDPAALEQDEVELVVQV
                     NGKLRGSIRVPKAADRAAIEELARANPNVQRFVAGHSVKKVVVVPGRLVNLVV"
     gene            33132..33989
                     /locus_tag="VIG70_00440"
     CDS             33132..33989
                     /locus_tag="VIG70_00440"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY8249047.1"
                     /translation="MKALLLALLLGVAPLQAFAQAAAVIDGVQMPAWRERDGNRVPLV
                     PGMELRAGDRILTGKEARVLVKLSEGSVVKLGENGTLRFAELQPSKELFRAALQVLEG
                     AFRFTTDLAAKARKREVTVRVEQVTIGIRGTDFWGRSRAERQIVCLLEGAIEVGAAGE
                     QPVTMDKPRQFYRRDKGKTEPVGFVEAKQLEQWALETDIQAGKGALRSGGRFSVQLAQ
                     ADKEEPLRAIRDQLHQEGYPVEIAQRKQGDKLTHIVRIRQLPSREEAQALADQLKGKF
                     GVKDPKVPG"
     gene            34062..36206
                     /locus_tag="VIG70_00445"
     CDS             34062..36206
                     /locus_tag="VIG70_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY8249048.1"
                     /translation="MSTLHLEHLNPEQLAAVTLPDEHALILAGAGSGKTRVLTTRIAW
                     LVKGARVAPSGILAVTFTNKAAREMLTRVGAMMTVNPRGMWIGTFHGLCNRMLRAHAQ
                     EAGLPRAFQILDSQDQLAAIKRLLKSMNVDEERFPAREVQYFVNDQKEEGVRSRDVPP
                     GDENTRRFAELYSAYDEQCQREGVVDFPELLLRSFELLNRNEILREHYRGRFRHILVD
                     EFQDTNRLQYRWLKLIGGKAACLFAVGDDDQSIYTFRGANVGNMADFEREFKVKHVVR
                     LEQNYRSHGNILDAANALIANNRNRLGKNLWTAAGKGEPVRVFEGESDNDEARWIVEE
                     VQALARDGTSLSQVAVLYRSNAQSRVVEHALFSQGIPYRVYGGLRFFERAEIKHALAY
                     LRLIATPDDDNAFLRVANFPPRGIGARTIEQLQDAAKQASVSLMKAADGTKAAPFRLL
                     IESLRNETQGLPLKEAVEVVIAKSGLVEHYKTEREGADRVENLAELVNAAAAFTEEER
                     VTESGEAVDPLIAFLTHAALEAGEHQAGEGQDALQMMTVHSAKGLEFDVVFMTGLEEG
                     LFPHEQSVTERDGLEEERRLAYVTITRARNRLYLSHAQTRMLHGQTRYNIPSRFLEEI
                     PQQLMKWLTPRFSRQKAFTPDYSRQTRTTLPKPRRDVGGFRIGQNVTHPKFGSGVIVD
                     AEGQGTDARVQVNFGATGVKWLAVAVAKLQAA"
     gene            complement(36216..37931)
                     /locus_tag="VIG70_00450"
     CDS             complement(36216..37931)
                     /locus_tag="VIG70_00450"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEY8249049.1"
                     /translation="MEFWNTAIFVGALLLVVSIVASDISSRLGAPLLLVFIALGMLAG
                     EDGPGGIRFDDFETAYVIGTLALAVIIFDGGMRTRRELFRVTLWPAAGLATVGVLVTA
                     LLVGVFAAWLLGLHWLQGMLIGAIVGSTDAAAVFALLRNAGATLQERVASTLEVESAS
                     NDPMAIFLTVVVLELLVAGKTQFEPGVLLAFVQQFGIGTVLGLAGGRLLVWLVNRLRL
                     HSGLYPLLAAAGGLFIFAAAQQAGGSGFLAIFLAGVVLGNSQLQAAQNILRVHDGLSW
                     LSQIVMFIILGLLVTPSEFLHIALPALAIAAFLILVARPLAVVASLLPFRFAWREQAF
                     VSWVGLRGAVPVVLALFPLLYGVENARLYFNVAFFIVLVSLVVQGWTIAPAARLLKLE
                     VPPSAEPAQRVTLDIPGHFEHEIVGYEVQAGAMVEARELGALELPAGLRIVSLLRDGV
                     PQPLEPRLKLAPRDYVYFLTQAHGVPLLNRLFDPHRAPERLEEHAYFGDFVLNGDAVL
                     GDLAAVYGIEVPAEHAGKTLAEYLNERAHGRVVVGDHAPLGNAELVVREVQDDRVTRV
                     GLKLR"
     gene            complement(37941..39236)
                     /gene="phoR"
                     /locus_tag="VIG70_00455"
     CDS             complement(37941..39236)
                     /gene="phoR"
                     /locus_tag="VIG70_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEY8249050.1"
                     /translation="MIYRNTLLALAALAVPAAFTGLVFGAAWGWGVLAVGLAALLFWH
                     TRHLSRLHRWLANPVAGKVPEGRGIWDEVLAALHRLERDTAKREEGLADALARMRRAV
                     QALPDGVVILDSANRIEWYNRTAEAQLGFEGRADIGQNIANLLREPEFIEYLQSSAHE
                     TPVRIDRGQALALSLQLIPFGQSQKMLLSRDVTHAERVDVMRRDFVANVSHELRTPLT
                     VLVGFLETVRELKLDPQRVRDYLGMMQEQAARMQRIIDDLLTLSALDSAPPATDDLRI
                     AVRPLLQRLGADAAALSAGRHVIRLEGETTSEILGVEGELTSALGNLVSNAIRYTPPG
                     GVVTLSWREGPDGARFTVEDTGIGIEAEHIPRLTERFYRVDRSRSRETGGTGLGLAIA
                     KHALARHDAVLEIQSEPGKGSRFSARFPARRLAVQPLVS"
     gene            complement(39233..39919)
                     /gene="phoB"
                     /locus_tag="VIG70_00460"
     CDS             complement(39233..39919)
                     /gene="phoB"
                     /locus_tag="VIG70_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY8249051.1"
                     /translation="MTATVLVVEDEPQIQELVAVNLEHIGHKVLRAASAEEAEAAIRN
                     ALPDVLVLDWMLPGESGVAFARRLRLDERTRELPILMLTARAMEQDKISGLEAGADDY
                     LTKPFSPKELAARIKAVLRRRAPQLSGDAVETEGLRLDPATRRISVQGKRVELSPSEF
                     RLLHFLMTHPGRVYTRAQLLDHVWGDHVFIEERTVDVHIRRLRKALEPSGHDRLIDTV
                     RGSGYGFRTD"
     gene            complement(39916..40596)
                     /gene="phoU"
                     /locus_tag="VIG70_00465"
     CDS             complement(39916..40596)
                     /gene="phoU"
                     /locus_tag="VIG70_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY8249052.1"
                     /translation="MTDHASKQYDLDLGSIRSRVLAMGGLVESQIRRAVDALTSGDLA
                     LSEEVIAADHRVNAMEVGLDEDCSQLIVRRQPAAGDLRMIFAILKTVTDLERIGDEAQ
                     KIARMAKNIHERGGPQAPPVVEVRHAAEAAISMLRRTLDCFARLDCDGAAEVIRDDAG
                     IDTEFRSILRQLITYMMEDPRTISTALEIVWIAKAIERIGDHAKNMAEYVIYIVKGTD
                     VRHTKIEA"
     gene            complement(40593..41366)
                     /gene="pstB"
                     /locus_tag="VIG70_00470"
     CDS             complement(40593..41366)
                     /gene="pstB"
                     /locus_tag="VIG70_00470"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY8249053.1"
                     /translation="MSEVLAAKAEAKGLSFYYGDFRALKNINMTVHERKVTALIGPSG
                     CGKSTFLRCFNRMHDLYPGNRYEGEIVLQPDNTNLLASAVDPIEVRMRIGMVFQKPNP
                     FPKSVFENVAYGLRVRGEGSRRRIEDKVEQALHDAALWNEVKDRLHQPAYNLSGGQQQ
                     RLCIARALATDPELLLFDEPTSALDPIATASIEELMHDLKERVTILIVTHNMQQAARV
                     SDFTAYMYLGDLIEFGVTDELFIKPKMKQTEDYITGRFG"
     gene            complement(41387..42295)
                     /gene="pstA"
                     /locus_tag="VIG70_00475"
     CDS             complement(41387..42295)
                     /gene="pstA"
                     /locus_tag="VIG70_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY8249054.1"
                     /translation="MKPIKDLIKKRKRWDAIFALCGLLALMIGVLTFAALFVDMALDG
                     FGRLSWDFFTSYPSRRPGQAGILSAWIGSTLVMLTTAFFAVPLGVAAGVYLEEYAAKN
                     WITDVIEINITNLAGVPSIVYGLLALGLFVYQFGFGQSILSAGLTLALLILPVVIVAT
                     REAIRSIPQVIREGSYALGATQWQTVRYHILPYSSAGILTGIIIGMARAIGETAPIIT
                     IGALTFIAFLPPAPIQSEPPFISFEWLMSPFTVMPIQMFNWTSRPEAAFHANAAAAGL
                     VLVIMTLAMNGLAIWLRYRLRRNLKW"
     gene            complement(42282..43196)
                     /gene="pstC"
                     /locus_tag="VIG70_00480"
     CDS             complement(42282..43196)
                     /gene="pstC"
                     /locus_tag="VIG70_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY8249055.1"
                     /translation="MPTPKGERLARRRARIVRERLIELLLFLAALVSVFTTVGIVYIL
                     VKESVVFFREVPIVNFLTDTQWTPLFDDAHFGIVVLLSGTLTSSAVALGIAIPLGTVI
                     AIYLSEFAPFRLREVAKPFLEMLGGVPTIVYGYFALLFVTPLLQRIYPELPGFNLLSA
                     GIVMGIMIIPYVSSISEDAMRAVPMSLREGSYAVGATRFQTATRVVVPAAFSGIASAY
                     ILGISRAVGETMILAVAAGMQPNLTFNPLEPAATITAFIVQVALGDLPHGSVGYQTIF
                     AAGLTLMLFTLVCNIGGHMLRKTFREAY"
     gene            complement(43229..>43523)
                     /locus_tag="VIG70_00485"
     CDS             complement(43229..>43523)
                     /locus_tag="VIG70_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein sphX"
                     /protein_id="HEY8249056.1"
                     /translation="EPSMEAVLKGSYTPLARPIFIYVNAKSLAKPEVKEFVEYYMKNG
                     AALATEVKYVPLPDSAYRVALEHVLKGKKGTVFGGVAEVGVTIEELLKREAKL"
BASE COUNT         7028 a        14430 c        14779 g         7286 t
ORIGIN      
        1 aggctgccga gcaggttggg catgaccgtg accggcttgc cggcgtgcag caccagccgc
       61 tcgcggatgc ggatgccgat gcggcggcag agatcctcga agtcggtgat ggtgcagtgg
      121 tggatgttgg gggtctcgta ccacgggtag ggaagggtct cggagaccgg catcttgccg
      181 agcgcgatct gcaggcgcgc gccccagtgg ccgaagttcg ggaagctgac gatgccctcg
      241 cggccgacgc gcagcatctc gcgcagcagg aattcggtgc ggtggatggt ctgcaaggtc
      301 tccgaaagga tcacgcagtc gaacgagccg tcggcgaaga gcgaaagccc gctctcgagg
      361 tccacctgca gcacgttgac gtcgttcgcg acgcacgcga gcacggcttc gtcctcgatc
      421 tcgacgccgt agcccggcgc ctcgcgctcc agccacagcg agcgcaggag cgcgccgtcg
      481 ccgagccgag gtccagcacg cgcgcgcctt gcggtaccca cttcacgatc gcctcctgat
      541 cgggcctcac aggtcgatat tcgcgaagta ggagcgcagc gccgcatggt agcgcgggtc
      601 ttccaggaga aacgcgtcgt gcccgcccgg agcgtcgagc tccagatacg agacgatgcg
      661 ccggttgtcg agcagcgcgc gcaccatctc gcgcgagcgc gccggcgtga agcgccagtc
      721 ggacttgaac gacacgacga ggaacgccgc cttcgcgcgc gccaacgcct tcgtaaggtc
      781 gccgccgaag tcggccgcag ggtcgaaata gtcgagcgcc ttggtgatgc gcaaataggt
      841 gttggcgtcg aaatagctcg agaacttgtc gccctggtag cgcaggtagg actcgacctc
      901 gaagtcgatg tcgaagtcga agccgggctg gccgcgccgc agcacgcggc cgaacttctc
      961 cgccatcgcc tcgcccgaga tgtaggtgat gtggccgatc atgcgcgcga tgcgcaggcc
     1021 gcgcaccggc accacgccct tgtcgtagta gtgcccgccg tggaaatccg ggtcggtcat
     1081 gatcgcctgg cgcgcgacct cgttgaacgc gatgttctgc gacgtgagct tggcggccgc
     1141 cgcgatcacg atcgagtggc ggatgcgctc ggggtacgac agcgtccact gcagcgcctg
     1201 catcgcgccg agcgagccgc cgatgaccgc ggcgaagcgc tcgatcccga gccggtcggc
     1261 gagtcggcgc tgcgcctcga cccagtcctc caccgtgacc accgggaaat ccgcgcccca
     1321 gggcttgccg gtggcgggat tgaccgacgc tgggccggtc gagccgaagc agctgccgat
     1381 gtagttcgag ccgacgacga agaagcggtt ggtgtcgagc ggcttgccgg ggccgaccag
     1441 gttgtcccac cacccgacat tgtctttttc tttttcatag aagcctgcca catggtggga
     1501 ggcgttcagg gcatggcaca cgagcacggc gttcgacttt gccgcgttca gcgtgccgta
     1561 ggtctcgtac gcaatctcgt acgaaggcag ctcggcgccg ccgcgcaaat gcagcggctc
     1621 gctgaaatgc gccttctgag cggttgcagc gccgaccgag gatggatcgt gtggagcgtt
     1681 cacagtctct ttagcggtat ttatatgttt acgacgcggc agtagccgcg tgggcgcccg
     1741 caagctgaca tcaaatcggc gccggcgcat tatagcggca gcccaaggag gagtcatgag
     1801 acccattctt gcaatagcgg ccttgctggc gtcgaacgcg gtgacggcga gcgagctggt
     1861 ggccgagcgc gtccagaagc tgacccgcgc cgtgcgctgg cagccggtgg cggaaattcc
     1921 ggtcggcttc aagacccacc acccgcaggg gatggcgaag atcggcgaca cgttcttcgt
     1981 ttcctcggtc gagatcacgg tgccgaccaa gcgcttcccc gagctcaagg acggctacga
     2041 ccgcgacacc ggcgagggcg tcgggcatct cttcaggatc gacgccaagg gcaatctgct
     2101 cggcgatctc aagctcggcg aaggctcgat ctatcacccc ggcggcatcg actacgacgg
     2161 ccgtcacctc tgggtcgcgg tggccgaata ccggccgaac agccgctcga tcatctaccg
     2221 cgtcgatccg cagacgatgc aggcgaccga gatgctgcgc ttcgccgacc acatcggcgg
     2281 catcgtgcac aacaccgacg atcgcaccct gcatggcgtg agctggggct cgcgccgcct
     2341 gtacaagtgg acgcttggcc gcgacggcaa agtgacgaat gcgggcgcac cgccggagaa
     2401 gctgcgcagg ccaaaccgct cgcactacat cgactaccag gattgccact acgtcggcgg
     2461 ccggcgcatg ctgtgctcgg gcctgaacaa ttaccggatg aaggccgatg ggccggcctt
     2521 cccgctcggt ggactggaga tcgtcgacct taagaccgac caggcggtct accaggtgcc
     2581 ggtcgagctg tggtcgccgt cgggcaagcc gatgacgcag aacccgttct gggtggaagc
     2641 gaccgacaac ggcctgcgcg cctacttcat gcctgacgac gacaagtcga cgctgttcgt
     2701 cttcgaggct gcggtcaaat aaggtaagtc gccgacaacg tcagtccgcg gcgcgcgccg
     2761 cgccggtgtc gatcagggct tgtatcttgt cgttcgagag gccggcttcg gccagaacct
     2821 cgcgcgtgtg ctcgcccagg cgcggcgcat ggcgccggta ctcgggcttc gattcggacc
     2881 actccgaggg cacggcgagc atgcggatgc gcccttcgct cgggtggtcg acgagctgga
     2941 ggtagccgat ggcgttcagg tgcgggtcgg cgacgatgtc gtccagcgag ttcatgcgct
     3001 gcaccgggat gtcgccgcgc tccagcgctt cgagccagaa cgcggtgggg cgctgcttca
     3061 tctcttccgc gatcatggcg tacgcggcct ggtagttctg gctgcgcgca tcgtgggtgg
     3121 cgaactccgg gcgcttgagc atctcgggcc ttccgatgat gtcgaagaac gacttccact
     3181 gcttgtcgtt gtagatcagc gcgcagacat agccgtcctg ggtctggtac ggccgccggt
     3241 cgggcgtcag gatgcgcaga tagccgggct cgcctttctg cggctcaaag gtgtagccgc
     3301 cgaggtggtc ggagagcacg atctgcagga ggtgctccca catcggcacg tcgacgcgct
     3361 ggcctttgcc cgtcttctcg cgatggtaaa gggcggcgct gaccgcgctc gcgacctgct
     3421 gcccgaccga gcggtcggcg acgatcatcg gcgcgtagcg cggctcgggc gcgccctgct
     3481 tctgcagcag ccacggcagc cccgaggcgc cctggatcat gtcgtcataa gcggctttgg
     3541 cggcataggg accgcgctgg ctgtagccga agcagccgac gtagatgatc ttcggattca
     3601 ccttcttcac ttcttcatag ccgagcttca ggcgcgtcat cgcctgcggc cggatgttgt
     3661 agaccagcac gtcgcttcgt ttcgcaagct gcaggcacgc ttcgcgtgca gcggcttgct
     3721 tcaaatcgag cgccaccgag cgcttgttgc gattgacgtt gaggaaaatc gcgcccatcc
     3781 ccgggttgcg gaacggccag gcctggcgga tcacgtcgcc ttcgagcggt tccaccttga
     3841 tcacgtcggc gccgaagtca ccgaggatct gggtcgcgta aggacccatc accacggtgg
     3901 tgagatcgag gacgcggacg ccgtgcagcg ggccgctcat gcgtcgatca ccatctcgag
     3961 cagcgcgtag gacagcgttt tgccgtgcgg gtcgatggcg agcgaggtcg tgacgccgct
     4021 gtcgagcgcg cgctcgcaga cgaactgcag cgcgtgcagc ccgggcacct cgaagcggcg
     4081 cacctcgccg ccgacgaggt gggcgaggtg gcgcttcacc gcctgcgcgg taacctcacg
     4141 gcgcagcagc tcgtagtcct cttcgcgata gggaaacagg gccagcgtca gcgtgtcgcc
     4201 cttgtcgccg gcgcgcgcgt gcgccagctc atgcagcctg cgcttcacgg tcctcgtcca
     4261 gcagcgtgac cttcggccgt acccgcgcgc gctcgagcag cagcgacacc acgccgatgc
     4321 gctcctgggc gtggcggcgc accccgccgc cccccgccgg tccgttgatc cacaaggcct
     4381 cgacttcggc gccgacgcgc tcggcgagct cgcgggtcgc ggttcgtgcc gctacgcgca
     4441 gcctcacttc ataaggtagc gaatgcgaaa aatcggtgcg atgcgcgctg tccatgccga
     4501 tcaggtcgac gcgcaggtcg gtgacgtcgt cgcgcagccg ctcgcgcacg atctcgcccg
     4561 cgagccgcgc gcgctccacc gcctttgcgc cggcgtagga aatctcgcct tcgccgacga
     4621 atcccgcgcg gtagcccacg ctcaccttgt aggtcggggt gcgcgggcgg ccgccggcgc
     4681 cccgcacggc gacccggtcc ggagcggact cctgcaggga aaccgtgctg aaatcggcga
     4741 ccacatccgg cgtgatatag ccgccgggat ccgtcacctc gtagaggagc tgctccttcg
     4801 cggtgcgcag cgtgatggcg ccgcccgtgc cggccacctt gccgagcacg ccgttgccct
     4861 ccgcgtcgac gtcgcaccag ggaaagccga gctgcgccag gttgggcacg tccttgaggc
     4921 cgggctcggc gaagtagccg ccggtgacct ggcccgcgca ttcgagcagg tgcccgatgg
     4981 ccgtggcgcg tccgaggcgc ggccagtcgt ccaggcgcca cttgaattcg tgcgccagcg
     5041 gcgcaacgaa gagcgacggg tcggcgacgc ggccggtgat cacgacctgc gcgccgctcg
     5101 cgagcgccgg aagcatctcc tcggcgccga aataagcgtt ggccgacacc agcgcgccgt
     5161 attcggacag cgccgcgccg cgcgcctcga tggtgcgcat gccgggctcg agcagcgaga
     5221 gcacgtcgtc gccgctgagc accgcgattc tgactttcag tccaaattca cgggaaagag
     5281 ccatgacgcg gcgcccggcg ccaagcgggt tcgccgcgcc catgttggtg acgatgcgga
     5341 agcggtggcg caccgcgagc ggcagcagcg cgcgcatgcg cgcctcgagc agcgggtcga
     5401 atcccgccgc cggatcggcg agcttgcgca gctgcgcgag cgcgatggtg cgctcggcga
     5461 ggcattcgag cgcgaggtag tcgagctcgc cctgcgaggc gaggatcacc gcgggctcga
     5521 gccggtcgcc ctggaatccg ctccccgcac caatccggat gctattcagg ctggattccg
     5581 gcatctttcg cgagtcttgc gaacttcgcg atctcggagc ggatgaagcc gccgagctgc
     5641 tccgggttca tcggcgcgac gtcggtgccc agcgaagcga agcgcgctat tacttccggc
     5701 ttggccaggg acttggcggt gatgtcgtgc agccgcgcga ccacggctgg cggcgtgccc
     5761 gccggcgcca ccagcgcgaa ccaggcgatc agctcgtagc cggcaagctc ttcggcgatg
     5821 gctgggacgt cgggcgcgag cgcggagcgc ttctgcgagg tgacggcgat gccgcgcagc
     5881 ttgccgccct tgatctgggc gagcgcgttg gccaggtcgg cgaaggtgaa gctgatctgg
     5941 ccgccgagca cgtcggtgat cgcctgcggc aggcccttgt agggcacgtc gatgaaatcg
     6001 atcttgccca tcgagcgcag catggcctgc gatacctgcg agcccgccga gccgtagccc
     6061 gcggagagtt tgccgggatt ggctttcgca tgagcgagaa gctccttgag gttcttcgcc
     6121 gggaagtcgg gccgcaccat gatcatgaag gagatcgtgc cgaggcgcgc gaccggcgcg
     6181 aagtccttca ccgggtcgta gttgagcttc ttgaacaggc tcgggttcgc ggcgagcggc
     6241 gtgttggtgc cgaccatgag cgtgtagccg tcgggcgcgg cgcgcgccac ctcggccgac
     6301 gcgatcgagc cctgcgcgcc gggcttgttc tcgaccacga atggctggcc gagcgactcc
     6361 tggagctgct ggccgaccac gcgcgcgacc tggtcggtcg cgctgccggc cgggaacggc
     6421 accaccagct tgaccggctt ggaggggtag gtctgagcgt gggcgagcgg aatggccagc
     6481 gcaacgagaa gagatgacag ccatttcatg aaaatgcctc ctaggcgttg agtttggcga
     6541 gcaggtcgcg cgttttcacc gggtcgctcg cccgcagcac gcgctgcgcg agcgcctgca
     6601 cctcggcgag gttggtgcgc aggatgcgct ccttgatcgc caggagctgc gacgggtgca
     6661 tcgagaagtt gcgcaggccc aggccgagca gcaggcgggt gagctgcagg tcgcccgcca
     6721 tttctccaca caccgcgact ggcacgccac cccgcgtcgc cgtctggatg gtgccggcga
     6781 cgagcgtcag caccgcgggg tgcagcgggt cgtagaggtg cgcgaccgag tcgtcggtgc
     6841 ggtcgatggc gagcgtgtac tggatcaggt cgttggtccc gatcgacagg aagtcgagcc
     6901 tgcgcatgaa catcggcagc gcgagcgccg cggcggggat ctcgatcatg ccgccgatcg
     6961 ggatgtcctg gtcgtagggc tggcggcgct cgtcgagctg cgccttggcc tgggcgatga
     7021 gcgcgagggt ctggtcgatc tcgtgggcgt gcgcgagcat cggcagcagc atgcgcactt
     7081 tcccgtagtg cgaggcccgc aggatcgcgc gcagttgcgt gaggaacatc tgcggctcgg
     7141 cgaggcacag gcggatcgcg cgcaggccca ttgccggatt gggcatggtg tgcgagccct
     7201 cgccgctgcc gttcagcgcc ttgtccgcgc ccagatcgag cgtgcgcagc accaccggcc
     7261 ggccctgcat cgccttcgcc acttcgcggt aggcctcgag ctgctcctcc tcgctcggca
     7321 ggtccttgcg attgaggaac atgaactcgg tgcggaacag gcccacgcct tcggcgccgg
     7381 cctcgagcac ctcgggcatg tcctgcggca gctcgatgtt ggcgtagagc tcgatcggcg
     7441 tgccgtcgac cgtggcggcg ggcgtggact tgaggcgcct gaggcgcttc ttctcggtct
     7501 tgaggtcggc ctggcgcccg cggtactcgg cgagcaccag cggatccgga ttgacgatca
     7561 gcaggccctg cgtgccgtcg acgatcagcg tgtcgccttc ggcgatcgtc tcgcgcgcgt
     7621 gatgcaggcc gacgaccgcg ggaatgccga gcgagcgcgc cacgatcgcg gtgtgcgagg
     7681 tgacgccgcc gacgtcggtg acgaacccgc cgaagcggtg gcgcttgaac aggatcatgt
     7741 cggcgggcga caggtcgtgt gccaccacaa tcagcttctc ctcctccgag atcgccggcg
     7801 ggctcagggt ctggccgccc atcagcgcct tgagcacgcg ctcgaccgcc tgctgcacgt
     7861 cggccttgcg ctcgcgcagg tacgggtcgt cgatctcctc gaagcgctcg accaggcgct
     7921 ccatgtgctg caccagggcc cattcggcgt tggcgcggcg cttgcggatc gtctcgcgcg
     7981 gcgccagcgc cagcgccgag tcgttgagga tcatgcggtg caggtcgagg aaggcctcga
     8041 actcggccgg cgacccggac gggatgtgcg ccttgagcgc ggcgagctcc tcctgggcgg
     8101 cgagcatcgc gcggtcgaag cgcatcactt cggcgtcgag cgcttccggc gcgacttcgt
     8161 agtgcgcgac ctccagccgg gcggtcgaga cgaggtgggc gttgccgacg gtgatgccgc
     8221 tcgacacggg gtacccgtga agaacgaaat tcattctccc tcgccgaact tgtctgcgat
     8281 cagcttggtg atcgcggcga tcgcctgctc ggcgtcggcg ccctcggcct cgatcagcac
     8341 cgaggtgccc tggccggcgg cgagcatcat cacgcccatg atgctcttcg cgttgacgcg
     8401 ccggccgctg cgcgagatcc agatctcgca cgggtaggtg ccggcgaggt gggtgagctt
     8461 cgccgcggcg cgcgcgtgca agccgagcct gttcacgatc gttgccgaga cgctaggcat
     8521 cgcagcagcg gtcgttgttc atgtgcacca cgccttcggc gccgcccgag agcgcgcgct
     8581 tgaccgcggc ggacagcgat ccgttgcgcc cggtcagcac gcgcagcagc atcggcagcg
     8641 aagcccccga gaccccctcg atgcggccgt cctcgagcag gcgcgacacc acgttgccgg
     8701 gggtggcgcc gaagatgtcg gtgagcacca gcaccccgtc gcccgaatcg aggctctcca
     8761 tgagctcgcg cgcgcgcagc accaggtcgt cgacgtcgtc ggcgcgccag acgctcagcg
     8821 ttgccacctg cttctgctcg tcgccgagga tctggctcgc gctcgtgagg agcgaggtgc
     8881 cgatgtcgcc gtgcgagacg atgagaacgc cgatcatcgg agcttctttt ccagggcatc
     8941 gaggaacatc ccggaaacat cgaacccggt ctggtcctgg atctcgcgga aacaggtcgg
     9001 gctcgtcacg ttcacctcgg tcagccagtc gccgatcaca tcgagaccca ccaccagcag
     9061 gcctctcttt gccagcacgg gtcccagggc agccgcgatc tcgcggtgcc tcgcggtgat
     9121 cggctgggcc acgccgcgcg ccccggcggc gaggttgccg cgcgagtctc ccgccttcgg
     9181 gatgcgcgcg aggcagtgcg gtaccaccgc gccatcgatc aggagcacgc gcttgtcgcc
     9241 ctgggagatc tccgggatat agcgctgcgc catcacgctg cggcgagcgc cctggctgat
     9301 ggtctcgaca atgacgttgc ggttcgggtc gtcggcgcgc acgcggaaca cgttctcgcc
     9361 gcccatcacg tcgaggcgct tcaccacgat gtcgcgctcg ctgtcgacga atgcctggat
     9421 gcgctcgggc tcgcgcgata cgagcgtcgg cgcagcgaag cgcgcgaact ccgcgatggc
     9481 gagcttctcg ttgtgatcgc ggatcgcgtc gggcgcgtta tacacgtgcg cgccttcgcg
     9541 cacggcctgc gaaagcagcc aggtgctggt cacgtattcg aggtcgaagg gcggatcctt
     9601 gcgcatcagc acggcgctga agtccgcgag cggccgctgc gcggtctcgt gggggcgaaa
     9661 ccagtcctcg tcgttgtcgg cgagcgaaat gcgcagcgcc gcggcgccga cgcggccctg
     9721 cagccagtgg aggctcgcct gctcgcacac ccagatggcg tggccgcgct tctgcgcggc
     9781 acgcatcatc gcgacggtgg tgtccttgta cgccttgagc gaatcaggcg ggtcgataac
     9841 gaaaaggaat tccaacccac cctacttgct ttgcgaaacc atgaactcca ccgcggcgcg
     9901 gatctctgcg tcgctcaggg ccgggttgcc ggctttcggc ggcatcgcgc ccttgccctt
     9961 gatcaccgac tgcaccatcg cgccggtgcc ggtcttgatg cgcggcgccc acgcttcctt
    10021 gtcgccgagc ttcggcgagc ccgcgaccga ctgctggtgg caggcgaagc agatcttgtc
    10081 gtagaccgcc ttgccgtcgg cggcgtggcc ggcatgggcc ggcgggggtt tttctttcgg
    10141 agcggccggc gccttgaagc tgccgccggc gaggttcgcc atgtgcacca ccgcgcgccg
    10201 gatttcgtcg tcggtgagcg aaggatcgcc cgccttcggc ggcatcgcgc ccttgccctt
    10261 gatcaccgac tccaccatgg cattcaggcc gtgcttgagg tgcggcgccc agtcgttctt
    10321 gtcgccgatc ttcggcgcct tggcgacgcc ggcctgatgg cacgcggagc acgtcgcctg
    10381 cacgatggct tccccggaac gcgcccccgg cgcggcggcg ggcgcgccga tctcgaaccg
    10441 cgcgaccggc tggatacgcg cggcgatcga ctcggcggtg agcgcctgcg gatcggcgct
    10501 ggggcggctc agcacgagct gcaccagaag aaggataccg atgatcggca ccaggaacgc
    10561 aagcaggatc acgacgatca actgctgcgg ggtcttgatg aacgactcgt gctcgtgcgc
    10621 ttccacgatt ggcttttcag tcttgtaaaa acggaattct agcttgaaag ctaacgcgtg
    10681 gacgcggaag ggaaaacggg ctatcctgcg cgtccgtttt tttagagcgc ccgtagctca
    10741 gcggatcaga gtattggcct ccgaagccaa gggtcgtggg ttcgaatccc gccgggcgcg
    10801 ccacacttcc ccggatttcc ctcaacgcac cgcgcgccgc acgaaatcgg tcaaccggct
    10861 tcgcagcgcc cagtcctgca gcgattgaat atggccggcc gccggcacga tccagagttc
    10921 cttcggctgt cccgcgagct cgtagagctg gcgcgagtgg tgcggcggca cgatgccgtc
    10981 gccctcgcca tggatcagca gcagcgcgac cgggctcacg gcgccgaccg agctgcgcgg
    11041 cgagtagtcg ttgtcgacgg tcaatgccgg cagccactgg aacggccacg tgaggaagaa
    11101 gccggccatt ttttccgtga cgatgaggcg gtagtcggcg aacgcgctgt cggcgatcac
    11161 cgcgcgcacg ctcgagcgat gggcgcctcg cgccgcgtag tggatcgcca gcgcgccgcc
    11221 gaggctctga ccgaacagca cgatgcgcct gggatcgacg tcgcgccgct cgagcagggc
    11281 gcgtagcgcc gcgtcgatgt cgagctgcac gccggccagc gacggcgcgc cgctcgatct
    11341 tccatagccg cggtagtcga gcacgaggac gttgaagccc tccgccggca tccaggcgac
    11401 ggcggcgtag tgcgtgctga tgttttccgc gttgccgtga aggaataaga ccgtggcctg
    11461 cggtgtgccg cgggccggca ggaaccaggt gaatagctcg acgccgtcgg cggcgcgcag
    11521 cggcagcacc tcatactcga gaccggcact tgccggcgtg tcgaccagca cgcgctgggg
    11581 ctggaagaag gtgttcgtgc agcccgccgt gagtgtgacg agcagcacaa atacaacgcg
    11641 cgtgaacggg gttggcaccg cgcgaatcgt acgccttgac agtgcccgtc cgcgcgccaa
    11701 gaatgggcca cgttcccgtc cccagtggaa cgcaacttag ggagaactcg aaatgaaaaa
    11761 actcgtagca gccgcggcga tcgccgcgct gttcccggca gttgccctcg cgcagcagaa
    11821 caacgttggc agctgtggct ggggctcgaa gttgatggac ggccagtccg gtgtctttcc
    11881 tcaggcgctg gcgggtatta ccaacgggac cttcggcaac cagaccttcg gcgtgtccac
    11941 cggcacctcg ggctgcacgc aggacggtac ggtgcgctcg acttggaaga ccgcgatgtt
    12001 catcgacggc aacaaggaaa ggctcgcccg cgacatgtca gtcggcggcg gcgaagcact
    12061 cgactcgctg gcgcacattc tcggcgtcga ccagacggac cgcgcggcgt tcaaccgcct
    12121 cgcccgcgac aacatggcgc gcatcttccc gagcgaaaac gccaacacgg agcaggtcgt
    12181 ctccgcgctg cgcgcggtgc tcatgacgga cgccaagctc gcgcgctaca cggtagcgct
    12241 ctaaagctcg gaaccgctgc acccgcaacg caaaaccctg tggccgtggc cacagggttt
    12301 ttttttcctc tggctgctgt ttccggcagg ggtcctcgcc gctgactacg ccggcgagct
    12361 ggtcgagcgc gcccgcagcc tgaagctcgc cgagcgcgcc gagtggcgca agctcatgca
    12421 ctacgttccc aacctgatcg cgccgggggt gcacggccag gtcgacagtc gcgcgttcta
    12481 caacgcgccc gacggcaaga acgacccggg cgccgagctc gaggccaccc tgctcagctt
    12541 cttctcgccg gcgccggaca gcgacgagcg gcagcatcca caatgccgct ttcccgcgcg
    12601 gtacggctgg cttgccgccg aactcggctt cgacgcgcgg cgcatgccgc acccggaatg
    12661 ccggcgctac cgcgagtggc gcacggcact cgacgcgaag cagctgacgc tggtcttcgc
    12721 ttcggcctac ctcaacaacc ccgggtccat gtacgggcac acgctgctgc gcgtggacgc
    12781 ggcggaccag gatgagcgca cgcgcctgct cgcctacacg atcagcttcg ccgccaacac
    12841 caacgagacc aacgggctga tctacgcggt aaaggggctg tttggcggct acgccggagt
    12901 gatatcgatg ctgccgtatc acgcgaaagt gcgcgaatac ggcgacctcg agaaccgcga
    12961 cctgtgggag tacgagctcg acttcaccgc ggaggaggtc gagcgggtgc tgcggcacgc
    13021 gtgggaactg ggcccgagct acttcgacta ctacttcttc gacgagaact gctcgtacca
    13081 cttgctgggc ctgctgcagg tcgcgcgccc ggacctcgag ctgaccgcgc cgttcccgtt
    13141 gtgggcgctg ccgatcgaca cggtgcgcgt gctgaccgag cggcccgggc tggtgcggcg
    13201 aatcacctac cgtccggcaa actcgacgat cctcggcgcg cgtctcgccg gcatgcggcc
    13261 ggaggagcgg cgcttcgcca gggacctggg actcggcacg ctgagcgcga gcgacgcggc
    13321 gctgcgtgcc ttgccgcccg agcgggccgc ggcggtgatc gaggcgggat acgactacct
    13381 gatctatcgg cgcaccacgg gcgtaagcga agtgccggac ggcggcgcgc tcgcccacga
    13441 tctcttggtg gcgcgcggcg cggtggacgc tccttcgcag gcgccgcgta tcgagcccgg
    13501 ggtgcgccct gaccagggcc accgcacctc gcgcctcgcg gccggcgtag ggcggcggga
    13561 gcgcgaggac ttcgtcgagc tcggcgtgcg cgcgacctac cacgacatcg tcgacgacga
    13621 cgcgggctac atcggcggcg cgcagatcga gttcttcaag ctgcgtgccc gtcgctacga
    13681 gcacggcgac gcgcgcatcg aaagcttcat cccggtggag atcttctcgc tctcgccgcg
    13741 cgacgagttc ttccagcccc gctccgcgcg cctcgccttc ggctggcagc gcaccttcac
    13801 ccgcagcggc gccgaaccgc tcgcgctcgg cgccgaaggc ggcttcggcg gcgcgtggaa
    13861 gcccaggact ggggcgcgct tctatgcaac cgtggaagcg gccgtccgcg cgcacgacgc
    13921 gttcgaggaa gggtacacgc tcggcgcggg cgcgcgcctc ggcgccctgg tcgatcccac
    13981 cgcgcgctgg cgcatccacg cctacgccca gcagctcggc tcgctcgccg gcgagcgcca
    14041 cgaccccggc gcgctcgtgc tggagcagag gttcacgctg acgcgcgata tggcgctgcg
    14101 cttcgacctc agccgccagc gcgaatccgg ccgcaccttc gataccgcgc tgctctcgtt
    14161 gcagctctac ttctgactta tcataggagc tgccgatcca ggcagtcctt cctcacggag
    14221 attcaagcca tgccaatgat tcgcgtcttc ggcgcggccg cgctgttcac gctggccacc
    14281 agcgccgccg cccagcagta cccgaaccgg aacatctcga tgatcgtgcc gtacgccgcg
    14341 ggcggcccga ccgataccgt ggcgcgcgtg acggcgcagg ccatgggcaa gccgctcggc
    14401 cagaccatca tcgtcgagaa ccggccgagc gccggcggca tcctcgggcc ggagcaggtg
    14461 aagaactcca agcccgacgg ctacacgatc ctcatccacc acatcggcat ggcgaccacg
    14521 ccggcgctgt acagaaacct gcgcttcaag ccgctggaag acttcgagcc gatcggcctg
    14581 atcaacgacg tgccgatgac catcatcggc aagccgcagc tcccggcgaa ggacttcaag
    14641 gaattcctcg cgtacatcaa gaagaacaag gacaaggtga gctacgccaa cgccggcatc
    14701 ggcgcggcct cgcacctgtg cgggatgctc ttcatgagcg cgatcgagac cgatttcctc
    14761 accgtgccgt acaagggcac tgcgcccgcg atgaacgatc tgctcggcgg ccaggtggac
    14821 ttcatgtgcg accagaccac caacacgact tcccagatca agagcggcaa ggtgaaggcc
    14881 tttgcggtca cttccgctaa gcgcgtgccc tcgctgccgg atattccgac gctcgcggaa
    14941 gtcggcgtca agggtgccga agtcggcatc tggcacggcc tgtacgcgcc gaagggcacg
    15001 ccgaagccgg tgctcgacaa gctggtgggc gccctccagg acgcactcaa ggatgaaggc
    15061 gtgcagaagc gcttctcgga cctcggcgcg acgacctacc ccgtgtcgat ggcgacgccc
    15121 gccgctctgc agaagcacct gaaggcggaa atggacaagt ggggaccgct gatcaagaaa
    15181 gccggcgtct acgccgacta gggaaggttt gaagaagtcg cccccgcgga ggcgggggcc
    15241 ccggtttctt ttttccccta gcgcatcagc gcgtactcgc cgccgcgctc gatcgcccgc
    15301 cggtacgccg gccgggcttg gatcttctcc aggaaattca tcaatcgcgg gcgcttctcg
    15361 tccagcccgc cgcgcgcccg cgacgcctcg agggggaagc tcatctgaat gtcggcggcg
    15421 ctgaattgcg cgccggcgaa ccattcgctt ttcccgagct cgctttccat gaagtccagg
    15481 ttgcggccga ggttcggcag cacgaacgtg gtgagcgcct tgtcggcgat ggcgcgcgcg
    15541 acgggccgca cgaaaaacgg cgacttggtc ttgatgcgat cgaacaggag cttgagcagc
    15601 agcggcggct gcgccgtccc ttcggcgaag tgcaaccaat atagatagcg cacgcgctcc
    15661 ggcgagccga atgccggcgc gagcttgccc gcgccgtagc ggtcagcgag gtgctcgacg
    15721 atcgcgccgg actcggcgag cgccacgccg ccgtcgacga tcaccggcga cttgcccagc
    15781 ggatgcaccg cgcgcagctc cggcggcgcg agcatggtct gccggtcgcg ctggtagcgc
    15841 ttgacttcgt attcgagccc gagctcctcg agcagccaca ggacgcgctg cgagcgcgag
    15901 ttgttgaggt ggtggacggt gatcataaaa aagccgccct caggcggctt ctggagtctg
    15961 cggattccta ggctacaccg ttcttcttga accaggccag catgcgcttc cagccgtctt
    16021 cggccgcttc cttgcggtag gtcggtcggt agtcagcatg gaaggcgtgc ggcatgcctt
    16081 cgtaaacgtg gatcgtggat ttcttgtcgt tgccgaacgc caacagcccg gcgcgcaggc
    16141 gctcgacgtg atccatcggg atgccctggt cggcactgcc gtagaggccg agtaccgggc
    16201 ggtccacctt gtctgtaacg tcgagcgggc cggtgggcat cgccggcggg atcggcatga
    16261 gcgagccgta ccaggcgacg cccgcgtcga tcttcttgtt gtgcgccgtg tacatccaca
    16321 ccatgcggcc gccgcggcac atgcccgtga tgccgagctt tcccgcgttc gcgcgcggat
    16381 gcttctcggc ccaggctacc actgcgtcga gatcggcgaa ggcctgctga tcggtgagct
    16441 ggttggccat cgcgatcgac tgcttgatgt cggttaccgt cttaaggtcg ccaatgcgga
    16501 acgtcggatc gagcgagact ccgaaatagc cagctttagc cagccgccgg cagatgtcct
    16561 tctggtactc gtgcaagccc cagatttccg gaatgacgag cactaccggg cgcttgccct
    16621 tgcccttggg caccgcgtaa tacgcgggta tctctccgat cttgacgtcg ccgccgtcga
    16681 ggccgtccat cgaggtcttg atcgcctgcg cgaggatcgg ctcggcggtc accgcgaagc
    16741 ccgcggcgat gcacgccgcg acgaagccgc gccggtcgac ttgcacgtcc ggcaccaggc
    16801 tgttcaggtc gtcgttcttc aggtccctgc gcgaatcttc catagcctcc tcccgttgtt
    16861 ggatacggtg atttacgtct gcaggcgccg gattattcca ccgatcgcac gtccttcggc
    16921 gggttttcgg cgaaccactg cgtcatttca gttcccggta ccggaagcag gcggtgagat
    16981 ggtcgttgac catcccggtc gcctgcatga aagcgtagca aatggtcgag ccgacgaagc
    17041 ggaagccgcg cttcttcagg tccttgctca tcgcgtccga ttccgccgtg cgcgccggga
    17101 tgtcccgtcc cttcctcgaa ttctggatcg gctggccgcc cacgaactgc catatgtaac
    17161 ggtcgaagga gccgaactcc ttctgcaccg cgagaaacgc gcgcgcgttg ctcaccgtgc
    17221 tctcgatctt caggcgattg cgcacgatgc cctcatcttt catgagaagc cttttgcggg
    17281 cagcgtcgaa gcgtgccacc ttcttcggat cgaagcgggc gaacgcgcgc tggtagttct
    17341 cgcgcttctt caggatcgtc gaccagctca ggcctgcttg cgcgccctcg aggatcagga
    17401 actcaaacag cacccgttcg tcgtgtacgg gtacgcccca ctcacggtcg tggtacgtga
    17461 tgtcgagctc ggtcgtcggc caggaacagc gtttcaccgc ttttcggggg gaatgccctt
    17521 gatgggggtt gaaaacttcg cgtgcgccgc cggatcgacg ggacggtgct tcaccttggt
    17581 gctcgcggga tccttcccga ggaaattgtc gcccttccag ttcttcgacg ggcgaaccgg
    17641 cctcggcacg aacccgccgc cgcagttggg gcacacgttg ccgaggacct tctcgacgca
    17701 cgccccgcag aacgtgcatt cgtagctgca aatgcgcgct tctggtgagt tcggcggcaa
    17761 ggccttgttg cagtgctcgc aggtcggacg aagttcgagc atcgtggtcc ccaaagacgc
    17821 ttagcgatcc ggttcagcgg cggcgcgccc gcgacgccgt tcgagcgtcc gctgcaaccg
    17881 gttgttaggc gtcatgtctt ggctttacct cgcagcatct ctgcgatcca ggccgcgcgt
    17941 cgctcgcgag tggcctgagc cttagctgcc cgaatgtgct cacctgcatc cctcgccatc
    18001 tctcggagac agccaccacg cggctgccag gcccttactc ttcgagagga tgctctccag
    18061 ttccttcggc gtagatgcgt ccgcgagctg aagctcgaga acgacacggt ctccgacatt
    18121 caggcgtgct gtctcgcaaa agggagttgt gaactccaaa aaccagtcac cgcttccctt
    18181 gccccaagct ttgatcgtac ggcgcccgaa aggatgccca ttggcggtac cttcgatcac
    18241 ggttgttcca gcaagacccc aaggctgcac atgccgaccc gaaataacga cgtagaccgg
    18301 caggcgtgga tccttgcgct ggagttgcgt tgagagttgc acttgagcac ccatgacgcc
    18361 taacgttagc cgtcaaggcg tgagcgcagc agcccccggc taacagctgg agacccgctg
    18421 agcgtgtcgg cgcccttcag aaaacaacac acttcattca cgtagcgttg ccaagccggc
    18481 tcatgcgaaa gtatcaggtg gttcctgctg tcgagcgcca cgaaccgcgc attcgggatg
    18541 gtgcgcgcga gcaaaaggcc ttgctcgaag ggtacacgcg catcaccgcg actgtggagg
    18601 atcaacgtag ggacggagac ctgcggcagc agatcgacga tgtcgatttc gccgaccgcg
    18661 tacagcaggc gaaccgcgtt ttcgggagtg gtggagacgc gctcgagatc attgagccat
    18721 cgcatttctt cgagcgtcgc atccgggacg atgagcgacg tgaacatctg gcgcgcggcc
    18781 ggattgtcct ggccccagcc ctcgcggatg agcgtaatcg acgcttccgc gcgagcgata
    18841 tcggctgagc tgccgcgttt gcgccatcct tgcacaaagc cgccggagag aatgagctgc
    18901 gaaacgcgct cggggtgtcg gacggcgtag gcaatgccga tcgatgcccc ttgagaaatg
    18961 cccagcaatg cgaaacggcc gagcccggcc gcatcaacga cggtctcgag atcttgcacg
    19021 aaggcctcga acgagaactg aacgtcccag tcggagaggc cattgccccg ttcgtcgtag
    19081 cggacaagct ggcactgggc cgataggcga cgaaaaagcg gcgcccacac cgggctccgc
    19141 cagtcatatt ccaggtgatt gagccagttg gcggtcttga ccagcgtcgg accgctcccg
    19201 gcagttgcca cggccaagtg cacgccatcg gccgttcggc agaacgtgat ctgctgctgc
    19261 acgtctgctt ccatgttgcc agcctggatg acaatcggcg aggcggcgag acgattggaa
    19321 ggtccttcgc gctcgcgcac gacaccgtcg aatctgtagc cgcaacgcgg aatcgtccgg
    19381 atggtcctct gctgttccgc accgtcgtgg accgcgcgcc gcaatgcact gatcctgctc
    19441 gtcagcgtgg cgtctgaaat gatgcggccg ttccagaccg cttcgatcag cttgtccttg
    19501 gtcagcaccc ggccgggatt ctggacaaga tgtaccagca ggtcgaaaac ctgcggttcg
    19561 acatgcacgg ccgcgccgcc ccgccgcagt tccctccggt cgacatcaac ctcgcagttg
    19621 ccgaagctgt agatcattgc cggtcaccaa tgcgggtcgg ttgggcgccg gccgaaatcc
    19681 gcggattctc agggaaaaat ccggactctc tgcaggactt cctgccgtct gggattcatt
    19741 ctagcgccat gccgaccccg ccgtggagca cgagaggtga cgatgagcca tccgaatccg
    19801 ttacgccgtg acgttctacg gcttaccttg tccgcggcgg cagctcttgc tgccgttcca
    19861 atgtctccag cgctcgcgca aagcacgaga aaagccacgt ttgtgctggt gcatccggcc
    19921 tggcacggcg cctggtactg gaaaaaggtg actccgctgc tccgggccaa gggctacgag
    19981 gtctacacgc ctaccctgac ggggctcggt gagctggccc atttggctcg acccgaggtc
    20041 gggcttcgga cgcatatcga ggatgtggtc agcgtcctcg agtatggaga tcttggcgat
    20101 gtgatcctgg tcgggaacag ctcggggggg atggtaatta cgggcgtcgc cgagcgtgct
    20161 cccgcgcgga tcgcgcaggt gatctacctc gatgccttcg taccgggaga cggccaatcc
    20221 ctcgtcgatc tcctcccgcc cgagcggaag catgcgttcg aggaattcgc aaaatccgag
    20281 ggcaagggtt ggctagtgcc gcgtttcgct ccgcttccct gggagcacat cgtgcgcaag
    20341 ctctggggcg tcaatgacga cgccgacgtg cggtggatgt tggcgcgctt ggttccgacg
    20401 ccgctcgggc acttcacgga accggtccag cgcacgaatc cggcgagcga caaactggtg
    20461 cggacctacg ttcgctgttt gcagttcaag caccccggtt tcgatcagca cgccgcgatg
    20521 gcgcaacgcg cgcccaactg gcgctatcgg gagatggcaa cgccccacct tccgccgatc
    20581 acgcatccac gagagctgac cgatctccta gtgcagctca cctagaagcg ctagcggcgg
    20641 ccggcgcttc cacacctgcg tcgcctgctc gaaggcgtgg gcgaactgca gcacgccgaa
    20701 atcgtcgcgg tagcggccga cgatctggat tcccacgggc aggccgtcgt cggtgaatcc
    20761 cgccggcacc gagatcgcgg ggtgcgtggt gagggtgatg tagtagcagc tcttcatcca
    20821 gtcgaggtag ttcgtcattt tcacgccggc gatctcggtc gggtagggct ggtcgaccga
    20881 aaacggcggc agctggttca ccgggcagac gaagaagtcg tatttttcca ggaacacgcg
    20941 catgcggtga tagagcgccg tgcgctggct ctccgccttg gcgatgtcgg cggcgctgcg
    21001 cttgaagccc tcctcggtgt tccagatcac cgtgtccttc agcttgtcgc ggtgctcttt
    21061 cagcagcgcg ccgccgcggt gcagccaggt cagtgcgcgc agagactcga aggcttcggt
    21121 cgcgccggag aaatcgggct cggcgtcttc gacgatgcag ccgagatcct tgaatgtctt
    21181 gcgctgcgac tcgagaacct tggtgactgc cggctcgacc ggcaagccgc caaggtcgcg
    21241 gctccaggcc acgcgggctt ttttgaagct gcgctttaga ggcttgttga agatcgagcc
    21301 gggctccatg atcgaagtcg gtgcgcgtcg gtcggggccg gcctggacgg aaagcaggaa
    21361 cgcggcgtcg gacacggtgc gcgccatcgg gccgagctcg ctcaggttgc tccacggata
    21421 gggatgcgga tacgccggga cgcggccggt ggaaggccgg aaaccgatca cgttgcaata
    21481 gttgccgggg ttgcggagcg aagcggcgaa gtcgccgccg tcggcgaatg gcaccatgcc
    21541 gcaggccacc gccacggcgg cgccgccgga ggagccgccg caggttttcg acaggtcgta
    21601 gggattgcgc gtggcgccga ataccttgtt gaaggtctgc gagccggcgc cgaactccgg
    21661 cgtgttggtc ttgccgatga ggatcgcgcc ggccgccttc aggcgctcgg ccatgatgtg
    21721 atcctcggtc ggcacgtggt ccttgtagac gagcgagccc caggtggtgc gcacgccttt
    21781 ggtggcgatc acgtctttga tcgcgatcgg caggccggcg aaggggccgg cgaaggcttt
    21841 ttttcgatcc tgggatttcg ctgcgttcaa tgcttggtcg ggcaagaagg tgacgatggc
    21901 gttcaccttc ggattgacgc gctcgatctg cgcgatgaag gcgcgcacga cctcggtcgc
    21961 ggacagcttg cgcgcgcgga tcaggcgcgc cagcgtccgc gccggcgtga agcagatttc
    22021 gctcagcaac cggcggtgca gcgtccgctg ccgtcgaact ccatcgtccg gccgctgagc
    22081 ggcacgtgca ccggcatctt cgccgctttc tggaacgccg cggtacggct ggtgggcgag
    22141 agcttgccgc cgacggtggc gggctcgttg gcgtgcgacg cgatcaccga gctcggccgc
    22201 accaggtcgt tcactacgta ggcggcctcg gtcgggccgg tggtgaacgg aaagccgccg
    22261 atgttcatca ccaccaggtt tgccttgtaa tgcccgcgca ccacgaggtc ctgctccgcg
    22321 gtgatgccgg tatcgccgga gagatacgcg atgagtccgt tgctgaagcg cagcacgaag
    22381 ccgccgggag gcccgacgta cgcggtcagg ccggcagcct cgaacatgcg cgcctgctcg
    22441 ggcggcagaa aggccggcgg caggccgttc gagtgcacgg ccgggacgct cgctaccatg
    22501 accttgccga ccatggtctg cgcgccgaag cgcaccaggc gcacgagctt cgccggatcg
    22561 cccccgaggc tcttcacctt ggccgagagg aacgagccca tctcgccgcc gacgatcacc
    22621 ggcgcctgct tcgccagcga aatattcacg ctgttcgagt tgggcgtcac gctgaccgag
    22681 aaatccggct tggggcaggc gccggcgttg gccgagggct ggatcaggtc gcccaggtgg
    22741 tcgccgtgga cgtggctcac caggatcgcg tcgatcctgc cgagccgcgg atcgttcggg
    22801 ccgcgcaccg tgcggccggc gtcgtagagg atgcgcgtgc cgtccggatc ctcgaagatg
    22861 agcgcgcggt cgagcgggca gaactcgccg tcgtggctgc cgagcggggt gatcttgacc
    22921 ttgggcgcct gcgggtccag gggcatgccg ccgcaggcgg cgagcgcgag gctcgcggca
    22981 atgatgagcg ctgttctcac gggtcctcct cctctggccc gtgatgttac agcagcacga
    23041 tgtcgaattg ctcctgggag taccccgatt cgacctgcac cgagatcggc ttgccgatga
    23101 agtccgacag catgccgagc gaggtcgatt cgtcctcgag cagcaggtcg atcaccgcct
    23161 gcccggcgat cacccggtac tcgcgtgcgt tgaaagcgcg cgcctcgcgc aggatctcgc
    23221 gcaggatgtc gtagcacacg gtgtgcgcgg tgcgcacctc gccgcggccg gcgcaggtgg
    23281 ggcagggctc gcacagcacg tgggcgagcg attcgcgcgt gcgcttgcgc gtcatctcca
    23341 ccaggccgag cgccgtgaag ccgttcagcg tgatgcgggt gcggtcccgc gccacggcgc
    23401 ggcgcagctc gtcgagcacc gccgcgcggt gctcggggct ttccatgtcg atgaagtcga
    23461 ggatgatgat gccgccgagg ttgcgcagcc ggagctggcg cgcgatcgcc tgcgccgcct
    23521 cgaggttggt cttgaacacc gtgtcgtcga agttgcggct gccgacgaag ccgccggtgt
    23581 tcacgtcgat ggtggtcagc gcctcggtct ggtcgatcat caggtagccg cccgacttga
    23641 gctcgacgcg gcgcgacagc gccttctcga tctcgggctc gacgttgtgc aggtcgaaca
    23701 gcggccgctc gccggtgtag tgctccaggc ggcttcgcac ctgcggcatg tagcgctcgg
    23761 cgaacgcggc gagcttctgg aagttctcgc gcgagtcgac ccgcacgcgc gcggtctcgg
    23821 cgctgaccat gtcgcgcagc acgcgctgcg cgagcgacag gtcctggtag acgaggagcg
    23881 gcggtgtagc gcccgcattt ggccccccgg agcgttgctg gatggccttc cacagttccc
    23941 gaagatacga gaggtcggcg cgcagctcct gctcgctcgc agtctcggcg agcgtgcgca
    24001 cgatgaatcc gcccttttcg tcggccggca gcaggtcctt gagccgctcg cgcagcgccg
    24061 cgcggccgtt ctcgtcctcg atcttctggg agatgccgat gtgcggatcc tgcggcaggt
    24121 agacgagcag ccgcccggcg atcgatacct gcgtggacag gcgcgccccc ttggtgccga
    24181 tcggatcctt caccacttgc accaggatcg gctggccttc gctcagggtt ttctcgatcg
    24241 gccggtcgcg ctggtcccag atgtcggcga cgtggaggaa ggcggcgcgc tccaggccga
    24301 cctcgacgaa cgccgactgc attcccggca gcacgcgcgc cacgcgcccg acgtagatgt
    24361 tgccgacgag gccgcggctc gccgcgcgct cgatcagcag ctcctgcacc acgcccgtgg
    24421 cggtgagcgc cacgcgcgtt tcctgcggcg tcacgttaat aaggatttct tccacgcagc
    24481 ttctcgagca gcaggctggt ctcgtacagc ggcagcccca tgactcccga gtagcttccg
    24541 cgcagctcca tcacgaagcg cccggcgcgg ccctggatgg cataggcgcc agccttgtcg
    24601 tcgccttctc cgctcgccgc gtagtgggcg atgtcgtcgt cggacagctt tttcaggacc
    24661 acttcggagc tcgagagcgc gaactcgacc tggtcgtagt tcttcagcgc caccccggtc
    24721 aggacctcgt ggcgctggcc cgagagcgcg gccagcatct ctgcggcctc ggcgcggtcg
    24781 cgcggcttgc ccaagatccg ctcctggaac gaaacggtgg tgtcggcggc gagcaccggc
    24841 gcaggcggca gattccgctg cagcatgcgg ctccagccgg cgtcggcctt ggcgcgcgcc
    24901 atgcgctcga cgtacgcggc cggcggctcg ccggcgagcg gcgcctcgtc gatgtcaggc
    24961 tcgctcgagc cgccgcgcgg gcgctcgcgg aaaagcagca ggtggtagcg cacgccgatc
    25021 tgggacagca gctcgcgccg ccgcgggctg cgcgaggcga gatagatgcc gcgctcggga
    25081 atggggatca tgcgcgatga taaggatgtc cggtcagcat ggtagccgcc cggtagatct
    25141 gctcgagcag cacgacctgc gcgagcgcgt ggggcagggt gagcgcggac aggcgcagca
    25201 gcagcgctgc gtcctttttc acgctttctt cgagcccgtc ggggccgccg atgacgaacg
    25261 tcgtctcggc cttcagtagg ggggcaaatt gagccgtcgt catgtccttt ccgcgctcgt
    25321 cgagcgccac cagccggcct ttcacgaagg agctccactt tccgggcttg gcatcgatcc
    25381 tcgaaacctc gtatccgcgc ggcatgcgct tcaggtactc gacgcaggcc gcctcggccc
    25441 agccgggcag cttagcggct acggcgacga cgtggagcct tggcacgcgc tttggctttc
    25501 ttcgggccct tcaacttcac cggcttgccg ccccagattt cctcgagatt gtagaagtcg
    25561 cgcaccgtcg gatgcatgat gtgcacgacc acgtcgccga ggtcgaccag cacccattcc
    25621 ccgctcgact cgccctcgac gccgtagacg cgggcgccga gctcgcgcac gcgctcttgc
    25681 acgcggtcgg cgagcgcctt cacctggcgg ttcgagtcgc cgctcgcgat caccacgcgc
    25741 tcgaacatcg agggcaggcg cgcggtgttg aacacgacga tgtcgtggcc cttcacgtcc
    25801 tcgagcgcct cgacgaccac ccgctgcttg acccggatat ccatcaggcg taaagcccgt
    25861 ggcgccggac atagtccgcc acggccggcg gcacgagcgc cgagaggtct tcgccgcggc
    25921 ccgcgcgcgc gcggatctcg ctcgccgaca ccggcatggg tttcatcgaa atgacggcaa
    25981 cccgcttgtc gagcgatgtt ccaggccgct cgaacaccgc gatcctggcg agcttgcgga
    26041 tgtcgtcggg gcgatgccag gcctcgaact tcgcgtactg gtcggcgccc atcaggagcc
    26101 acagctccgt ggcggcgccg agctcgagcc tgagctcctt cagcgtgtcg atggtgtagc
    26161 ccgaggcgcc cggtgcgagc tcgcgggcat cgatttcaca ggatttgtta tctgcaaggg
    26221 cgagccggag catggcgatc cggtgctcgc cgctcgccac cgccggcgtg cggtagcgcg
    26281 gcgcgccggt cggcatgaag aggagtttct cgagctcgag ctcgtcgagc gcagcgcgcg
    26341 ccatcgcgag gtgcgcgtta tgcacggggt cgaacgtgcc gcccaaaata ccgatgctca
    26401 gttgcggatt tctccgtgcc cgagaaccac gtatttctgg gtggtcagac cttcgaggcc
    26461 gacgggcccg cgtgcgtgca gcttgtcggt ggagatgccg atctcggcgc ccaggccgta
    26521 ctcgtagccg tcggcgaagc gcgtcgaggc gttccacatc accgagctcg agtcgacctc
    26581 gcgcaggaag cgctcggcgc gggcgcgatc ctgcgccacg atggcgtcgg tgtgctgcga
    26641 gccgtacttg gcgatgtgcg cgatcgcctc gtccagcccg tccaccacgc gaatcgacac
    26701 gaccggcgcg agccactcgg tgtagtagtc gctgtccgtc gctttttttc catccacgaa
    26761 ctcgagcgcg gcggcgtccg cgcgcatctc gacgcccttg ctcttgaaga tgccggaaat
    26821 gcgcggcagc acgcgctgcg cgatcccgga ggaaacgagc agggtctcca tcgtgttgca
    26881 cgtgccgtag cgctgggtct tggcgttgtc ggcgatgcgc accgccatct ccgggtcggc
    26941 gctgtcgtcg atgtagacgt ggcacacgcc gtcgaggtgc ttgatcatcg ggatgcgcga
    27001 ctcgcgcgcg aggcgctcga tcagctcctt gccgccgcgc ggcacgatga ggtcgacgtg
    27061 ctcgcgcatg gtgatgagct cgccgaccgc cgcgcggtcg gtggtgccga cgagctgcac
    27121 cgcggcctcg ggaagctgcg ctgctttcaa gccggcgcgc acgcaggcgg cgatcgcgcg
    27181 gttcgactcg atcgcttccg acccgccgcg caggatgcag gcgttgcccg cctttacgca
    27241 aagcgcggca gcgtccgcgg tcacgttggg ccgcgactcg tagatgatgc cgatgacgcc
    27301 gatcggcacg cgcattttcg cgacctcgat gccggtgggc cgcttcacgc gctcggagat
    27361 cgcgccgacc gggtcgggaa gcgacgccac ctgctcgacg ccggcggcca tttgctcggc
    27421 gagcgccggc gtgagcgtca gccggtcgac gaacgccgcg tcgcagccgt ctttcttcgc
    27481 tttggcgacg tccagcgcgt tggcttttga gagatccttg gaacgtgaac ggatttcttg
    27541 cgccactgcc tgaagcgcgc ggttcttggc gccggtctcg gcgcgcgcga gctcccgcgc
    27601 cgcggcgcgc gccgcgcgtc ccatctcctg catcgcggaa tgcaggtctc cctgggcatg
    27661 tttgttgcgc ttgagctgca tgggcgcatt gtaatggttc aggacgcggt catcgtcggc
    27721 ggagggctcg cagggctcgc ctgcgccgtg gcgcttggcg acgcgggcgt gcgcgtgacc
    27781 ctgctcgagc gcgaaaaacg cctcggcggc cgcgcgcgca gctggaccga cgcagcgagc
    27841 ggcgacccga tcgacctcgg gccgcacgtg gtgcacagcg agtatcgcaa catgctcgcc
    27901 ttcctcgagc ggctcggcac ccgggatttg atctgctggc agcccgggaa gctgatcacg
    27961 ttctcgtcgg gaatggtgct gcggcaccgg cggctgcccg cgcccttcag cctgctgccg
    28021 gatctcgtga ggggctcggg cctttccgcg cgcgatctct ggtccaacaa ccgcgtgacc
    28081 tggcgcgcga tgcggttcga cgaagccgat gtgcccgagc tcgaccgcgt gagcgcgctc
    28141 gaatatctgc gagccctcgg cgtcagcgag ccgatgatcg aatggttctg gtccttcgcc
    28201 gcgctctcgg tcatgaacgt gccgctcgag cgctgctcgg cggcggcgct gctgcgcgtg
    28261 cattcgcagc tcatcggcca tcgccggctg catttcggct ttccggcggt ggccttgagc
    28321 gagctgttcg cggcgcaggc cgccaatgcg atcgagcgcg cgggagggcg tgttctcctc
    28381 gaggcggagg tagctgaaat aaatgatgga gtcgttctgc gcgatggcgc gcgcattccc
    28441 gccgaccacg tggtcgcggc cgtgccgccg caggacctga agaagctggg gctcgccgct
    28501 cctgcggtcg agccgagtcc gtacatcagc tcgtacctct ggttcgaccg caagctgacc
    28561 agcgagcggt tctgggcgct gctcggcgcc ctaaagcacc tgaacacgga cttctacgac
    28621 ctttccaaca tccgccgcgg ctggtcggcg cggccctcgg tgatcgcgtc caacatcatc
    28681 tacagccatc gcgccggcac gatgagcgac gcggaaatcg tcgcggcgac gcagcgcgag
    28741 atcgcgcaat tcgttccgga ggctgcacgc tcgcgcgtgg tgcacgcgac cgtgaaccgc
    28801 atcgcgatgg cgattccctg ccccctgccg ggaagcgaga gcgagcgtcc tgccgcttcg
    28861 cattgcgccg gcatttcgtt ggccggcgac tggacgcgga ccggcttgcc gtgctcgatg
    28921 gagggcgcgg tgcgctcggg atggctcgcg gccgaagaag tgctgcgcgc ggcggggcgg
    28981 ccgcgcgccc tggcgcttgc gccgcgtccg gccgacggcc tggtcgggct ggttcagcga
    29041 ctcaggtcga gtgcagctcg agcccgagct tggcgaactc ctcccacggc tcgcccacgc
    29101 ccacaccctt gatcgcccgg tcgatcgccg cggcgcggct gatcgcctgc tcgatcgccg
    29161 cgggcggata gcggcgcgcc ttgtcgagca ctgcctgcat cgccttgctc gtgcgctcgc
    29221 cgccggcaag cgcaaacagc gcgctcgaga tcgtgaacag cagataggtc ggcgcttcgc
    29281 cttcgccgcg caatccgtcc aggatgcgca ggtagcgcgc cgtgtcgccg ccgaggagcg
    29341 cctcggcggc gacatgggtg tcgaaccgcg ccacgtcggc gaccgcgccg cgcacggttt
    29401 ccagggtgag ctcgccttcg ggcgcgagca gggcgagctt ctgcacctcc tggtgtgcgg
    29461 cgagcaggtt gccttcgacg cggtcggcga gatactcgag cacctcgcgc gaagcgctct
    29521 gcttctgcag cgcgagccgc ttctcaagcc acgccggaag ggccgcgcgc gtcacgggct
    29581 ggacttccac gatcacgccg tgcttgttga gcgcgaggaa ccagggcgca tcccaccagc
    29641 cgccgccctc gggccgcggc atggtgagca ggagcagcgt ctccggattc gtgcgctcgc
    29701 accaggcgac gatcgcctcg gccgcctgtg cgttgacctt gccgctcgcg agcctcagct
    29761 cgacgatctt cttcgtgccg aaaagcgaga gggtggcgcc ggcgtggcgc agctcgctcc
    29821 agtcgaacga gcgccccggc tcgtacacct cgcgctcggt aaagccgttc ttgcgcgccg
    29881 cgacgcgcac cgcgtcggcg gcctcgagga caagcagcgc ttcgtcgccg aggattgcgt
    29941 aggccgcgcc gagattcttc gccagatggg cggggagctg ttcctgggta cgaagtcgcg
    30001 gcattcgtcc ttctactgct acgggcgctt ggcggccgcc aggcggcgca tgatctgctg
    30061 caccatgtcg acctgcatgt cgcggaacag gagctgttcc tcctgctcct tggcgaggat
    30121 ctcggcgtcg ttgaaggtca cgtcgcgcgt gagctggatc gtgttcggcg gcacgtactc
    30181 ggcgcccttg ccgtcgtgga cgcgaaagcc cacgcggtag cgcagctgga actcgcgtac
    30241 ccggccggtg ccggtcagcg acaggatctc cttctggcgc gtctcctcgg tgaactgcat
    30301 caccgcgtcg gccttgcccg gatcgtccac cacgcgcgct ttagtcccgg cctggatggt
    30361 gcgcttgagg tcgagcgcga tgccgctggt cgcgctcggc acgtagagcg tctcgaacgg
    30421 tacttcggcg gtcccgcgca gccgaaagcc gcagcccgcc aggagcagcg ccaacgcgag
    30481 cgccagggct cttctagacg acaagattga ccagccgtcc cggcaccacg accaccttct
    30541 tgacgctgtg gccggcgacg aagcgctgca cgttcggatt ggcgcgcgcg agctcctcga
    30601 tcgccgcgcg gtcggcggcc ttgggcacgc gaatcgagcc gcgcagcttg ccgttcacct
    30661 gcaccaccag ctcgacctcg tcctgctcga gcgccgccgg gtcgggctcg ggccacggcg
    30721 cctccatgat gtcctcgccg aacttgagtt cgcgccacag gtggtgcgcg atgtgcggcg
    30781 cgatcggcga gagcagccgc agcaggatcg agaggccttc tttagaggtt gcatcccatt
    30841 cagggagcgc ttcgatcgca ttgaggatct tcatgcacgc ggaggcgacg gtgttgaatt
    30901 tcttgcgcac gatgtcgtcg ttcgccttct tcagctccag gtggacctgg tagcggatct
    30961 tccgctcgat gctctcggcg cacgtcgccc gcgagaactt gaaggcgaaa ccccagacct
    31021 tgcgcagaaa gttgtaggag ccttcgacgc tggcgtcgct ccattccagc gtgctggtcg
    31081 gcggcgacgt gaacatcgtg tagaagcgcg cggtgtccgc gccgaacttg tcgatcagcg
    31141 cctgcggatc gacgccgtta ttcttcgact tggacatcgt gccgatgccg tcgtagacca
    31201 ccgcccttcc gtccttcgcc ctggcggaaa cgatggttcc cttcgcgtcg cgcaccacct
    31261 cgacgtccgc cgggttgatc cactcgcgcc ggccgctggt gtcggtgtaa tacgactcgg
    31321 cgagcaccat gccctgcgtg agcaggttgg tgaacggctc gtcgacgccg cgcatcaggc
    31381 cggtctcttt ctgcatcgcg cgctggaaga agcgcgagta gagcaggtgc aggatggcgt
    31441 gctcgatgcc gccgatgtat tgatcgaccg gcatccagta gtcggcgcgc tcgtcggcca
    31501 ttgcgcccgc cacctccggc gagcagaagc gcacgaagta ccaggacgag tcgacgaacg
    31561 tgtccatggt gtcggtctcg cgcttcgccg gcctgccgca cgacgggcac ttggtgtcga
    31621 cgaacgatgc gagcttgttg agcgggttgc cggtgccgtc gggcaccacg tcctcgggca
    31681 ggcgcaccgg aagctgctcg tcgggcaccg gcacgtcgcc gcacgacggg caatgcacga
    31741 tcgggatcgg cgtgccccag tagcgctggc gcgagatgcc ccagtcgcgc aagcgccatt
    31801 gaatttgctt ttctcccaga gatttcctct ggagatcagc ggcgatggcg tcggtcgcct
    31861 ccctgtagga aaggccgtcg tacttcccgg agttgatgca agtcccgtat tcggcgtact
    31921 cgggcttcca gggggccggg accgtgtcgc cgagcggatg acggatcacc ggcttaatcg
    31981 gcagcttgtg cttgagcgcg aactcgtagt cgcgctcgtc gtgcgccggc acccccatca
    32041 ccgcgccttc gccgtagccc atcagcacgt agttcgcgac ccagaccggc agcttttcgc
    32101 ccgagagcgg atgggtgacg aaaagcccgg tgggcatccc ctttttctcg atttgcgcca
    32161 gctcggcctc catcgccgga ccgcgcttgc attcgtcgac gaaggctcgg actttagaat
    32221 ctttttgaga agcaaaaact gcaatcgggt gctccgccgc aaccgccatg aaggtcgcgc
    32281 ccatcagggt gtcggcgcgg gtggtgaaca cccatagggt ttggggttga ttgtttaact
    32341 gataaggaaa tccaatccgc acgccttcgg acttgccgat ccagttcttc tgcatcagct
    32401 tgacctgctc gggccagccg ggcaggtcgt cgagcgccgc gagcagctcc tcggcgtacg
    32461 cggtgatgcg caggaagtac atcgggatct cgcgcttctc gaccggcgcg ccggtgcgcc
    32521 agccgcggcc ctcgatcacc tgctcgttcg cgagcaccgt ctggtcgacc gggtcccagt
    32581 tgacgacgcc ggtcttcttg taggcgaggc ccttgcggaa caggcgcgtg aacagccact
    32641 ggttccactt gtagtagtcg ggcctgcagg tggcgagctc gcggctccag tcgagcgcga
    32701 agccgaggct cttcagctgc gccttcatgg tggcgatatt gctgtaggtc cacttcgccg
    32761 gcggcacctt gttcgccatc gccgcgttct cggcgggcag gccgaacgca tcccagccca
    32821 tcggctgcag cacgttgcag ccgcgcatgc ggtagtagcg cgtgaggacg tcgccgatgg
    32881 tgtagttgcg cacgtgcccc atgtgcagct tccccgaggg ataggggaac atggacaggc
    32941 agtagaattt cggcttggcg ggatcttcga gcgagcggaa cgaatcgcgg ctcgcccaga
    33001 agtcctgcgc ctctcgctca accgctgccg gatcgtagac gtcttgcatg tgactttttt
    33061 tgcggaagcc gcggattatc gcatccattg cccgcgccca agccgtataa ggcgcgtcag
    33121 ggagtgaccc catgaaagcg ctacttcttg cgttgcttct cggcgtcgcg ccgctccagg
    33181 cgttcgcgca ggcggcggcg gtgatcgatg gcgtgcagat gccggcctgg cgcgagcgcg
    33241 acggcaatcg cgtgccgctg gtccccggga tggagctgcg cgcgggcgac cgcatcctca
    33301 ccggcaagga ggcgcgcgtg ctcgtcaagc tctccgaggg cagcgtggtg aagctcggcg
    33361 agaacggcac cctgcggttc gccgagctgc agccctcgaa ggagctgttc cgcgccgcgc
    33421 tccaggtgct cgagggcgcg tttcgcttca ccaccgacct cgccgccaag gcacgcaagc
    33481 gcgaagtcac ggttcgcgtc gagcaggtga cgatcggcat ccgcggcacc gacttctggg
    33541 ggcgctcgcg cgccgagcgc cagatcgtgt gcctgctcga gggtgcgatc gaggtcggcg
    33601 ccgcgggcga gcagccggtg acgatggaca agccgcggca gttctaccgg cgcgacaagg
    33661 gcaagacgga gccggtcggc ttcgtcgagg cgaagcagct cgagcaatgg gcgctggaga
    33721 ccgacatcca ggccggcaag ggcgccttgc gcagcggcgg gcggttcagc gtgcagctcg
    33781 cgcaggcgga caaggaagag ccgctgcgcg cgatccgcga ccagctgcac caggagggat
    33841 atcccgtgga gatcgcgcag cgcaagcagg gcgacaagct gacccacatc gtgcggatcc
    33901 ggcagcttcc ctcgcgcgag gaagcgcaag ccctggctga tcagctcaaa ggcaagttcg
    33961 gagtgaagga ccccaaagtt cctggttgag ccgcattggc gggcttgtaa ttcaagctcg
    34021 ctgggcgggc ttgtaattca agcccgcgat aaaatcgcgg cttgtcgacg cttcacctcg
    34081 aacacctcaa cccggaacag ctcgccgcag taaccctgcc ggacgagcac gcgctgatcc
    34141 tcgccggtgc gggcagcggc aagacgcgcg tgctcaccac gcgcatcgcc tggctggtga
    34201 aaggcgctcg cgtcgcgccc tcgggaatcc tcgccgtcac cttcaccaac aaggcggcgc
    34261 gcgagatgct gacccgggtc ggggcgatga tgacggtgaa cccgcgcggc atgtggatcg
    34321 gcaccttcca cggcctgtgc aaccgcatgc tgcgcgccca cgcgcaggaa gccgggctgc
    34381 cgcgggcgtt ccagatcctc gactcgcagg accagctcgc cgcgatcaag cgcctcttga
    34441 aaagcatgaa cgtggacgag gagcgcttcc cggcgcgcga ggtgcagtac ttcgtcaacg
    34501 accagaagga agagggggtg cgctcgcgcg acgtgccgcc gggcgacgag aacacccggc
    34561 gcttcgccga gctctactcg gcctacgacg agcagtgcca gcgcgaaggc gtggtggatt
    34621 ttccggaatt gctcctgcga agcttcgaat tgttgaaccg caacgagatc ctgcgcgagc
    34681 actaccgcgg ccgtttccgc catatcctgg tggacgagtt ccaggatacg aaccggctgc
    34741 agtaccggtg gctcaagctg atcggaggaa aggccgcctg cctgttcgcg gtcggggacg
    34801 acgaccagag catctacacg ttccgcggcg ccaacgtcgg caacatggcc gatttcgagc
    34861 gcgagttcaa ggtgaagcac gtcgtgcgcc tggagcagaa ctaccgctcg cacggcaaca
    34921 tcctcgacgc cgccaacgcg ctcatcgcaa acaaccgcaa tcgcctcggc aagaacctgt
    34981 ggaccgcggc gggcaagggc gagccggtgc gcgtgttcga aggcgagtcc gacaacgacg
    35041 aggcacgctg gatcgtggag gaggtacagg cgctggcgcg agacggcacc tccctttcgc
    35101 aggtggcggt gctctaccgc tcgaacgcgc aatcgcgtgt cgtcgagcac gcgctgttct
    35161 cgcagggcat cccctaccgc gtctacggcg gcctgcgctt cttcgagcgc gccgagatca
    35221 agcacgcact cgcctacctg cggctcatcg ccacgcccga cgacgacaac gcgttcctgc
    35281 gtgtcgcgaa cttcccgccg cgcggcatcg gcgcgcgcac catcgagcag ctccaggacg
    35341 ccgcgaaaca ggcgtcggtt tcgctgatga aggctgcgga cggcaccaag gctgcgccat
    35401 tccgcctctt gattgaaagc ctcagaaacg aaacccaggg cctgccgctc aaggaggcgg
    35461 tcgaagtggt gatcgcaaaa agcggcctgg tcgagcatta caagaccgag cgcgagggcg
    35521 ccgaccgggt cgagaacctg gccgagctgg tgaacgccgc cgcggcgttt accgaagagg
    35581 agcgcgtcac cgagtcgggc gaggcggtcg atcccctgat tgccttcctc acccacgccg
    35641 cgctggaagc gggcgagcac caggcgggcg aaggccagga cgcgctccag atgatgacag
    35701 tgcactcggc gaagggcctc gagttcgacg tggtcttcat gaccgggctg gaagagggcc
    35761 tctttccgca cgagcagagc gtgacggagc gcgacggcct ggaagaggag cgtcgcctcg
    35821 cctacgtgac gatcacgcgc gcgcgcaacc gcctctacct ttcgcatgcg cagacgcgta
    35881 tgctgcacgg ccagacgcgc tacaacatcc cgtcgcgctt cctggaagaa atcccgcagc
    35941 agctcatgaa gtggctgacg ccgcgctttt cacggcagaa agcctttacc ccggattact
    36001 cccgtcaaac gaggaccact ttgccaaaac caagacgcga tgtcggagga ttcaggatcg
    36061 gccagaatgt tacgcatccg aaattcggca gcggcgtgat cgtcgacgcc gaaggccagg
    36121 ggaccgacgc gcgcgtgcag gtgaacttcg gcgcgacggg cgtgaagtgg ctcgcggtcg
    36181 ccgtggcgaa gctgcaggca gcctaggcgc cgtattcagc gcagcttcag acccacccgc
    36241 gtcacgcggt cgtcctgcac ttcgcgcacc acgagctcgg cgttgccgag cggcgcgtgg
    36301 tcgccgacca ccacccggcc gtgcgcgcgc tcgttcaagt actcggcgag ggtcttgccc
    36361 gcgtgctccg ccgggacctc gatgccgtac accgcggcga gatcgccgag caccgcgtcg
    36421 ccgttcagca cgaagtcgcc gaagtaggcg tgctcctcga gacgctcggg ggcgcgatgc
    36481 gggtcgaaca ggcggttcag gagcggcacg ccgtgcgcct gcgtgaggaa gtacacgtag
    36541 tcgcgcggcg cgagcttgag ccggggctcg agcggctgcg gcacgccgtc gcgcaggaga
    36601 ctgacgatcc gcagcccggc gggcagctcg agcgctccca gttcgcgcgc ctcgaccatg
    36661 gctccggcct gcacctcgta gccgacgatc tcgtgctcga agtggcccgg gatgtcgagc
    36721 gtgacgcgct gcgcgggttc tgcggacggc ggcacctcga gcttcagaag ccgcgccgcc
    36781 ggcgcgatgg tccagccttg caccacgagc gacaccagca cgatgaagaa ggcgacgttg
    36841 aagtacagac gcgcgttctc gaccccgtag aggaggggaa agagcgcaag caccaccggc
    36901 accgccccgc gcagcccgac ccagctgacg aaggcctgct cgcgccaggc gaagcggaac
    36961 ggcagcaggc tcgccaccac ggcgagcggc cgcgcgacga gtatcaggaa cgccgcgatc
    37021 gcgagcgcgg gcagcgcgat gtggaggaac tcggagggcg tgaccagcag cccgaggatg
    37081 atgaacatca cgatctggct cagccaggag agtccgtcat gcacgcgcag gatgttctgc
    37141 gccgcttgca gctgcgagtt gccgagcacc acgccggcaa ggaagatggc aagaaagccg
    37201 ctgccgcccg cttgctgggc ggcagcgaag atgaagagcc cgccggccgc cgcgagcaga
    37261 gggtagagcc cggagtgcag gcgcagccgg ttgacgagcc acaccagcag ccggccgcct
    37321 gccaggccga gcacggtgcc gatgccgaac tgctgcacga acgcaaggag cacgccgggc
    37381 tcgaactggg tcttgccggc gaccagcagc tcgagcacca cgaccgtgag gaagatggcc
    37441 atcggatcgt tgctcgccga ttccacctcc agggtcgaag cgacccgctc ctggagcgtg
    37501 gcgcctgcat tgcgcaacag cgcgaacacc gccgcggcgt cggtcgaacc gacgatggcc
    37561 ccgatcaaca tgccctgcag ccagtgcagg ccgagcagcc aggctgcgaa aacgccgacg
    37621 agcagcgccg tgaccagcac cccgaccgtg gcgaggcccg ccgccggcca cagggtgacg
    37681 cggaagagct cgcgccgggt gcgcatgccg ccgtcgaaga tgatgacggc gagcgccagc
    37741 gtgccgatca cataggcggt ctcgaaatcg tcgaagcgga tgccgcccgg cccgtcctcg
    37801 cccgccagca taccgagcgc gatgaagacc agcagcagcg gcgcacccag acgcgaggag
    37861 atgtcgcttg ccacgatgct gacgacgagc agcagcgccc ccacgaaaat cgcggtgttc
    37921 cagaactcca cacggcgaat ttagctgacg agcggctgta ccgcgagccg ccgcgccgga
    37981 aagcgcgcgc tgaagcggct gcccttgccg ggctcgctct ggatctcgag gaccgcgtcg
    38041 tggcgcgcga gcgcatgctt ggcgatcgcc aggccgaggc ccgtgccgcc ggtctcgcgc
    38101 gagcggctgc gatcgacccg gtagaagcgc tcggtgagcc gcgggatgtg ctcggcctcg
    38161 atgccgatgc cggtgtcctc gaccgtgaag cgggcgccgt ccggaccttc gcgccagctg
    38221 agcgtgacca cgccgcccgg cggggtgtag cggatcgcgt tgctcaccag gttgccgagc
    38281 gcgctcgtca gctcgccttc cacgccgagg atctcgctcg tcgtctcgcc ttcgaggcgg
    38341 atcacgtggc gcccggctga cagcgccgcg gcgtcggcgc ccaggcgctg gagcagcggc
    38401 cgtaccgcga tgcgcaggtc gtccgtggcg ggcggcgcgg agtcgagcgc cgacagcgtc
    38461 aggagatcgt cgatgatgcg ctgcatgcgc gccgcctgct cctgcatcat cccgaggtag
    38521 tcgcgcacgc gctgcggatc gagcttcagc tcgcgcaccg tctcgaggaa gcccaccagc
    38581 acggtgagcg gcgtgcgcag ctcgtgcgag acgttagcga cgaagtcgcg ccgcatgaca
    38641 tcgacgcgct ctgcgtgggt gacgtcgcgc gacagcagca tcttctgcga ctgcccgaag
    38701 gggatgagct gcagcgaaag cgcgagcgcc tgcccccggt cgatgcgcac cggggtctcg
    38761 tgcgcgctcg attgcaggta ctcgatgaat tcgggctcgc gcagcaggtt ggcgatgttc
    38821 tggccgatgt cggcgcggcc ctcgaagccg agctgcgcct cggcggtgcg gttgtaccac
    38881 tcgatgcggt tcgcactgtc caggatcacc acgccgtcgg gcagcgcctg caccgcgcgg
    38941 cgcattcggg cgagcgcgtc ggcgaggcct tcctcgcgct ttgccgtgtc gcgctccagg
    39001 cggtgcagcg cggcgagcac ttcgtcccag atgccccggc cttcgggcac cttgccggcc
    39061 acggggttgg cgagccagcg atgtaggcgc gagaggtggc gtgtgtgcca gaagaggagc
    39121 gcagccaagc cgaccgccag cacaccccag ccccatgcgg cgccgaaaac cagcccggtg
    39181 aaggccgcgg gcacggcgag cgccgcgagc gcgagaaggg tattgcggta gatcaatcgg
    39241 tgcggaagcc gtaccccgag ccgcgtaccg tgtcgatcag gcggtcgtgg ccgctcggct
    39301 cgagcgcctt gcgcaggcgc cggatgtgca cgtcgaccgt gcgctcctcg atgaacacgt
    39361 gatcccccca gacatggtcc agaagctgtg cgcgagtata gacgcgcccg ggatgggtca
    39421 tcaggaagtg gagcagccgg aactcggagg gcgagagctc gacgcgcttg ccctggacgc
    39481 tgatgcggcg cgtggccgga tcgaggcgca gtccctcggt ctcgaccgcg tcgcccgaaa
    39541 gctgcggcgc gcggcgccgc agcaccgcct tgatgcgcgc ggcgagctct ttcggcgaaa
    39601 agggcttggt gaggtagtcg tcggcgccgg cctccaggcc ggagatcttg tcctgctcca
    39661 tggcgcgcgc cgtcagcatc aggatcggca gctcgcgagt gcgctcgtcc aggcgcaggc
    39721 gccgcgcgaa cgccaccccg gactcgccgg ggagcatcca gtcgagcacc agcacgtcgg
    39781 gcagcgcgtt gcggatcgcc gcctcggcct cctcggcgct cgccgcgcgc agcaccttgt
    39841 ggccgatgtg ctcgaggttg accgcgacca gctcctggat ctgcggctcg tcttcgacca
    39901 cgaggaccgt ggccgtcatg cttctatctt cgtgtggcgc acgtcggtgc ctttgacgat
    39961 gtagatcaca tactccgcca tgttcttggc gtggtcgccg atgcgctcga tcgctttggc
    40021 gatccacacg atctccagcg ccgtgctgat agtgcgcggg tcttccatca tgtaggtgat
    40081 gagctggcgc aggatcgagc ggaactcggt gtcgatgccg gcgtcgtcgc ggatcacctc
    40141 cgccgctccg tcgcaatcca ggcgcgcgaa gcagtcgagc gtgcgccgca gcatcgagat
    40201 cgccgcctcc gcggcgtggc gcacctcgac caccggcggc gcctgggggc cgccgcgctc
    40261 gtggatgttc ttcgccatgc gcgcgatctt ctgcgcctcg tcgccgatgc gctcgaggtc
    40321 ggtcaccgtc ttcaggattg cgaagatcat gcgcaggtcg cccgccgccg gctggcggcg
    40381 cacgatgagc tggctgcagt cctcgtcgag ccccacctcc atcgcgttca cgcggtggtc
    40441 ggcggcgatc acttcttcgg agagcgcgag gtcgccgctc gtcagcgcgt cgacggcgcg
    40501 gcggatctgc gattccacca ggccgcccat cgccagcacg cgcgagcgga tcgagccgag
    40561 gtcgaggtcg tactgcttgg aagcgtgatc ggtcaaccga atcgtccagt tatgtagtct
    40621 tcagtctgct tcatcttggg tttgatgaag agctcatcgg tcaccccgaa ctcgatcagg
    40681 tcgcccaggt acatgtaggc ggtgaagtcc gagacgcgcg cggcctgctg catgttgtgc
    40741 gtcacgatga ggatggtgac gcgctccttc aagtcgtgca tcagctcctc gatcgaggcc
    40801 gtggcgatcg ggtccagggc ggaggtgggt tcatcgaaca gcaataattc aggatccgtc
    40861 gcaagcgccc gcgcgatgca caggcgctgc tgctgcccac cggagagatt gtacgccggc
    40921 tggtgcaggc ggtccttgac ctcgttccac agcgcggcgt cgtgcagcgc ctgctcgacc
    40981 ttgtcctcga tgcgccggcg cgagccctcg ccgcgcacgc gcagcccgta ggcgacgttc
    41041 tcgaacaccg acttcgggaa ggggttcggc ttctggaaca ccatgccgat cctcatgcgc
    41101 acttcgatcg gatcgacggc gctcgcgagc aggttggtgt tgtcgggctg cagcacgatc
    41161 tcgccctcgt agcggttgcc gggatagagg tcgtgcatgc ggttgaagca gcgcaggaag
    41221 gtcgacttgc cgcagcccga cgggccgatc agcgcggtga ccttgcgctc gtgcaccgtc
    41281 atgttgatgt tcttcagcgc gcgaaagtcg ccgtagtaga aggacaggcc tttcgcctcg
    41341 gccttggccg ccagtacttc gctcatgtgg gtctcgggtg ccgacattac cacttgaggt
    41401 tgcgtcgcaa tcgatagcgc agccatatgg cgagcccatt catggcaagg gtcatgatga
    41461 ccagcacgag gccggccgcc gcggcattgg cgtggaacgc cgcctcgggg cgcgaggtcc
    41521 agttgaacat ctggatcggc atcacggtga agggcgacat cagccattcg aacgagatga
    41581 acggcggctc ggactggatc ggtgccggcg gcaggaaagc gatgaaggtg agcgcgccga
    41641 tggtgatgat gggcgcggtc tcgccgatgg cgcgcgccat tccgatgatg atcccggtca
    41701 ggatgccggc cgaggagtag ggcaggatgt ggtagcgcac cgtctgccac tgcgtcgcgc
    41761 cgagcgcgta cgagccctcg cggatcacct gcgggatcga gcggatcgcc tcgcgcgtcg
    41821 cgacgatgac caccggcagg atgaggagcg caagcgtcag cccggccgac aggatcgact
    41881 ggccgaaccc gaactggtag acgaacaggc ccagcgcgag cagtccgtag acgatcgacg
    41941 gcacgccggc gaggttggtg atgttgatct cgatcacgtc ggtgatccag ttcttcgccg
    42001 cgtactcctc gaggtagacg ccggcggcga cccccagcgg caccgcgaag aaggcggtgg
    42061 tgagcatcac cagcgtcgag ccgatccagg cggagagaat tcccgcctgc cccgggcggc
    42121 gcgacggata gctggtgaag aagtcccacg acaggcggcc gaagccgtcg agcgccatgt
    42181 cgacgaacag cgccgcaaag gtgagcacgc cgatcatcag cgcgagcagg ccgcacagcg
    42241 cgaagatcgc gtcccagcgc tttcgcttct tgatgaggtc cttaataggc ttcacggaat
    42301 gtcttgcgca gcatgtggcc gccgatgttg cacacgagcg taaacagcat gagcgtgagc
    42361 cccgcggcga agatcgtctg gtagccgacc gagccgtgcg gcaggtcgcc gagcgccacc
    42421 tgcacgatga aggcggtgat ggtcgccgcg ggctcgagcg ggttgaaggt gagattgggc
    42481 tgcatgccgg cggcgaccgc caggatcatg gtctcgccca ccgcgcgcga gatgcccagg
    42541 atgtacgccg aagcgatgcc cgagaacgcc gccggcacca ccacgcgggt cgcggtctgg
    42601 aagcgcgtcg cgcccaccgc gtaggagccc tcgcgcaggc tcatcggcac cgcgcgcatc
    42661 gcgtcctcgg agatcgagct cacgtacggg atgatcatga tccccatcac gatgccggcg
    42721 gagagcaggt tgaagccggg cagctcggga tagatcctct gcaggagggg cgtgacgaag
    42781 agcagcgcga agtagccgta gacgatggtc ggcacgccgc cgagcatctc gaggaagggc
    42841 ttcgccacct cgcgcagccg gaagggcgcg aactcggaga ggtagatcgc gatcaccgtg
    42901 ccgagcggga tcgcgatccc gagggccacc gccgagctcg tgagcgtgcc cgagagcagc
    42961 accacgatgc cgaaatgcgc gtcgtcgaag agcggcgtcc actgcgtgtc ggtgaggaaa
    43021 ttcacgatcg gcacttcgcg gaagaacacc accgattcct tgaccaggat gtagacgatg
    43081 ccgacggtgg tgaataccga gaccagcgcg gcgagaaaca ggaggagctc gatcagccgc
    43141 tcgcggacga tgcgtgcgcg ccgccgcgcc aggcgctcac ccttcggggt tggcatcaac
    43201 cccgattcta tagaaagcga agcctgcttc agagcttcgc ttcgcgcttg agcagctctt
    43261 cgatcgtcac gccgacttcg gccacgccgc cgaacaccgt tcccttcttg cccttgagca
    43321 cgtgctccaa ggccacgcgg taggccgagt cgggtagcgg cacatatttc acttccgtcg
    43381 ccagcgccgc gccgttcttc atgtagtact cgacgaactc cttcacttcc ggcttggcga
    43441 gcgactttgc gttcacataa atgaatatcg ggcgcgcgag cggggtgtag ctgcccttca
    43501 gcaccgcttc catcgacggc tcg
//
LOCUS       DATBSZ010000006         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1025587, whole genome shotgun sequence.
ACCESSION   DATBSZ010000006 DATBSZ010000000
VERSION     DATBSZ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025587"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /gene="msrB"
                     /locus_tag="VIG70_00490"
     CDS             <1..345
                     /gene="msrB"
                     /locus_tag="VIG70_00490"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY8249057.1"
                     /translation="YRVLREEGTERAGSSPLNDEKRAGIFACAGCGLPVFTSEMKYES
                     GTGWPSFFTTIPGVFDQKKDFYLIYPRTEYHCKRCGGHHGHVFDDGPPPTNQRWCNNG
                     VSLKFIPKDSKA"
     gene            complement(406..2094)
                     /locus_tag="VIG70_00495"
     CDS             complement(406..2094)
                     /locus_tag="VIG70_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEY8249058.1"
                     /translation="MLRQTLGIMRELPRLHEIASVFIRHGLGDFVQRIGIAGVLERAG
                     QILHRSPVADAVTLDPAQRMRMALEELGPTFVKLGQVMATRVDLFPPRWIAEFEKLHA
                     DVPAVPFELLLPELTRSLGRSPFEVFREIDTRAHGAASIAQVHRARLQDGTPVVLKVR
                     RPGVRETIDADLRLLRRVSELIESEMPEARRYRPAEIAEQFAKSLEREADFAVETRNI
                     ERFAKNFAGDPHIVIPRIYPEYTSDVLLVQEHVDGIPATDLAAVEKSNLDKKLLAARG
                     VDGFLKMILLDGFFHADPHPGNVFYIPGNRIVVIDFGMVGRLSPQRRAQVIDLLGGLA
                     RMAEEPMLEVLLDWAGDAYVDETKLAADVNEMVFDYEGVALKDIRVGNVIRQFAAIVR
                     QHSIVLPSDLSLMFKALITLEGLGRQYDPDFHIINHLTPLVKSALAERYQPGELVRRG
                     RGMVTEFMNVVGGVPRDLARFLREARRGKTRIDLDLKRLDSFGRQLDRTLDRVTVGIM
                     TASLVIGSAIVSTVHDGPTLWGIPVLPAIGLLGYVLAFLNSLWIVYGIWRSGRE"
     gene            complement(2104..>2457)
                     /locus_tag="VIG70_00500"
     CDS             complement(2104..>2457)
                     /locus_tag="VIG70_00500"
                     /inference="COORDINATES: protein motif:HMM:NF042805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6781 family protein"
                     /protein_id="HEY8249059.1"
                     /translation="SALRQMVATGRSISDNEVKQALAGLKKIEEDFVATVSQVASSAS
                     ERTRPELRALVERAAHAGTETGRRTAHLMKEFTFSGMELAGQFSVRFAQLASGVLAGM
                     ADALEKSAAERQRKT"
BASE COUNT          400 a          841 c          829 g          387 t
ORIGIN      
        1 taccgcgtgc tgcgcgagga ggggaccgag cgtgccggca gcagcccgct gaacgacgag
       61 aagcgcgccg gcatcttcgc ttgcgccggc tgcgggctgc cggtcttcac ctccgagatg
      121 aagtacgaat ccggcaccgg ctggccgagc ttcttcacca ccatcccggg cgtgttcgac
      181 cagaagaagg atttctacct gatctacccg cgcaccgagt accactgcaa gcgctgcggc
      241 ggccatcacg gccacgtgtt cgacgacggc ccgccgccga ccaaccagcg ctggtgcaac
      301 aacggcgtct cgctgaaatt catccccaaa gacagcaagg cgtaattcgg ggtcagggtc
      361 acaactcgtt tacctaattc gcaccctgac cccgaattag gtttgctact ccctgcccga
      421 gcgccagatc ccgtagacga tccaaaggct gttcagaaaa gcaagaacat agccgagtaa
      481 cccgatcgcc ggcaggacgg ggatgcccca gagcgtcggg ccgtcgtgca cggtggagac
      541 gatcgccgag ccgatgacga gcgacgcggt catgatgccc acggtgacgc ggtcgagcgt
      601 gcggtcgagc tggcggccga agctgtcgag gcgcttgagg tccaggtcga tgcgcgtctt
      661 gccgcgacgc gcctcgcgca ggaaacgcgc gaggtcgcgc ggcacgccgc cgacgacgtt
      721 catgaactcc gtgaccatgc cgcgcccgcg gcgcaccagc tcgcccggct ggtagcgctc
      781 ggcgagcgcg ctcttcacca gcggcgtgag gtggttgatg atgtggaagt cggggtcgta
      841 ctgccggccc agaccctcga gggtgatgag cgccttgaac atcagcgaca ggtccgaggg
      901 cagcacgatc gagtgctggc gcacgatggc ggcgaactgg cggatcacgt tgcccacccg
      961 gatgtccttg agcgccacgc cctcgtagtc gaacaccatc tcgttcacgt cggcggcaag
     1021 cttcgtctcg tcgacgtagg cgtcccccgc ccagtcgagc agcacctcca gcatcggctc
     1081 ctcggccatg cgcgccagcc cgccgaggag atcgatcacc tgcgcgcggc gctgcggcga
     1141 gagccgcccg accatgccga aatcgatcac cacgatgcgg ttgcccggga tgtagaacac
     1201 gttgcccggg tgcgggtcgg cgtggaagaa gccgtcgagc aggatcatct tgaggaaccc
     1261 gtcgacgccg cgggcggcca ggagtttctt gtctaagttg gatttttcta ctgcagcaag
     1321 gtccgtggcc gggatcccgt cgacgtgctc ctgcaccagc agcacgtcgc tcgtgtattc
     1381 ggggtagatg cgagggatca cgatatgcgg gtcgccggcg aagttcttgg cgaagcgctc
     1441 gatgttgcgc gtctcgaccg cgaaatcggc ctcgcgctcg agcgacttgg cgaactgctc
     1501 ggcgatctcg gccgggcggt agcgccgcgc ttcgggcatc tcggactcga tcagctcgga
     1561 cacgcggcgc agcaggcgca ggtcggcatc gatggtctcg cgcacgcccg gccgcctcac
     1621 cttcagcacc accggcgtgc cgtcctgcag ccgggcgcgg tgcacctgcg cgatcgaagc
     1681 cgcgccgtgg gcgcgcgtgt cgatctcgcg gaacacctcg aacggcgagc ggccgagcga
     1741 gcgcgtgagc tcgggcagca gcagctcgaa cggcaccgcc ggcacgtcgg cgtgcagctt
     1801 ctcgaactcc gcgatccagc gcggcgggaa caggtccacg cgcgtcgcca tcacctggcc
     1861 gagcttcacg aacgtcggcc cgagctcctc cagcgccatg cgcatgcgct gcgccgggtc
     1921 gagcgtgacc gcgtcggcga ccgggctgcg gtgcaggatc tggccggcgc gctcgagcac
     1981 gccggcgatg ccgatgcgct ggacgaagtc gcccaggccg tggcggatga agaccgacgc
     2041 gatctcgtgc agccgcggca gctcgcgcat gatgccgagg gtttgccgca acatcggccg
     2101 gggctaggtt tttctctgtc tttcggcggc ggacttctcg agcgcgtcgg ccatgcccgc
     2161 gagcacgccg ctcgcgagct gagcaaaccg gacgctgaat tgccccgcga gctccatgcc
     2221 cgagaaggtg aactccttca tcaggtgcgc ggtccggcgc ccggtctcgg tgccggcgtg
     2281 cgcggcgcgc tcgaccagcg cgcgcagctc ggggcgcgtg cgctcgctcg ccgagctcgc
     2341 cacctggctc acggtggcga cgaaatcctc ctcgatcttc ttcaacccgg cgagcgcctg
     2401 cttcacctcg ttgtcgctga tgctgcggcc ggtggccacc atctggcgca gcgccga
//
LOCUS       DATBSZ010000007         5962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_957394, whole genome shotgun sequence.
ACCESSION   DATBSZ010000007 DATBSZ010000000
VERSION     DATBSZ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957394"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VIG70_00505"
     CDS             complement(<1..603)
                     /locus_tag="VIG70_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8249060.1"
                     /translation="MKHHEAVRGHVAASVLRARPDETAGAVLARLAHDKPVSVELVLV
                     VNGHGKLKGVVSLGRLFAAPAAVPLKEILDSKSPRVTADTDQEHAASLALHHGVDALP
                     VVDDEGHALGVMPAQALLQVLRREHVEDLHRLAGIQREAAQARHAIEDPPLRRVRHRL
                     PWLLVGLAGSALATAAMAAMEVTLQANVAIAFFVPALVYLA"
     gene            639..1208
                     /locus_tag="VIG70_00510"
     CDS             639..1208
                     /locus_tag="VIG70_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8249061.1"
                     /translation="MTWVKRARQAKPTIDWSTMAEYPAHLAVRRRLADGRSVVIRPIR
                     PQDEVRQREFLDRLSGDTRRRRFMKRADAVDGGLAHFHSDIDYDRHMGFVCESGDTLV
                     GDARYVANADRRSCEFGVVVADDWHHTGIAQLLMDALIRAARARGFETMEGIVLEDNR
                     DMLDFVRELGFERAPAAEQPAIVRIVKKL"
     gene            complement(1265..1753)
                     /locus_tag="VIG70_00515"
     CDS             complement(1265..1753)
                     /locus_tag="VIG70_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY8249062.1"
                     /translation="MSFAVWLTGHSGSGKSAIARELVRLLHERRVDVSVLESDVMRTQ
                     LTPFPRYDDADRDFFYATLGEIGFLLVEKQKAVVFDATANRRAYRDAARKRIARFAEV
                     FVDTPLEVCRARDPKGLYRKGMSIPYEPPQAAELVVRGDQGTPPESAARIVALLEDRG
                     WV"
     gene            complement(1750..2829)
                     /locus_tag="VIG70_00520"
     CDS             complement(1750..2829)
                     /locus_tag="VIG70_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8249063.1"
                     /translation="MPSAFTPQALAAYLGAVHGRAVSGVRVTPLGGGRQGDKGYGYGI
                     PLRVDYTLDGVLRRAVVETVRPGPFGHEHMADRAQSLLWAHGAFGKLPRHVVSLDVGA
                     VRSSGALVQLGNAEELFMLAEFVEGAEYADDLLKLRAGKALSPLDETRADALCDYLVE
                     IHRVAGPDPGLYVRRIRELLGHGECIFGVADSYPGDGPVPNSVLESIEKSCVEWRWKL
                     KARSRRLRQVHGDFHPWNIMFRRGTDFSVLDRSRGEWGDAADDVTCLTLNYLFFSLQR
                     SGRLEGDFERLWRRFWDRYLERSGDREILEVAAPFLAFRGLVMANPLWYPALDTGVRR
                     KLFNFIHAVLAEERFEPASANAYCA"
     gene            2912..3268
                     /locus_tag="VIG70_00525"
     CDS             2912..3268
                     /locus_tag="VIG70_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEY8249064.1"
                     /translation="MKPWQRDQLRRALEQRRETLLEELKRDAARLREERSGDIGDIGD
                     ESVADLLVDLDQADLSRDAGELREIEAARRRLDGGTYGICADCGAAIDFERLRAEPAA
                     ARCVECQARHEKTYRR"
     gene            complement(3256..4128)
                     /locus_tag="VIG70_00530"
     CDS             complement(3256..4128)
                     /locus_tag="VIG70_00530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8249065.1"
                     /translation="MEPAFRRYIEQCPDAVLVTDTAGRIVYANPAFERMTGYASSELA
                     GRTPALLKSGMHEADFYRELWSALLAGREFRALFANRKKSGEPYYEDKIIRPLFDADG
                     RATHFVSFGRDATERAREFDRLLHAATHDSLTDLPNRSLFLDRLGQALRHAARREEQL
                     TLAILDIDRFRDTNNRCGHMAGDTVLQAVAARTRGCVREADTVARIGGDELAVILLDA
                     TEAQAGPVLAKIVAANAAPVRHDGANIRATVSVGASGYPRDAQDADALRKQADLAMYA
                     AKAAGGNRYHFYRR"
     gene            4210..5553
                     /locus_tag="VIG70_00535"
     CDS             4210..5553
                     /locus_tag="VIG70_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8249066.1"
                     /translation="MRALLALLVLLAAPPTAAQAPDFTALVREGAAVVNLSGTLGGVL
                     PDLPLAEEEAEDTGILRDFLRREYGPAPETRSLGSGFVIDPSGYIITNAHLVASTAEI
                     SVRLADRREFEARVLGLDPASDIALLKIEASGLATVRIGDPAKLRPGEWVAAIGSPFG
                     LERSVTAGIVSALGRTLPQESFVAFIQTDVAVNPGNSGGPLFNLRGEVVGVNSGFYSD
                     TGGYIGLSFAIPIDVAMEVAAELRAYGKVTRGRIGVRLQELTAELARALKLPRAAGAL
                     VVETFPGYAAARAGVRPGDVIVRFDGQPVEAEAELVRLTARATPGATVDVELLRFGAP
                     LLLRLKVDATRVATLPAAVRPDGEPLGLQLAPLDRRQRERMRLESGLLVERARAASQR
                     AGLMRGDVIVSVNGKAVSSAEAFAAVLQAAGKGGTVALLVQRDGVRQFVPLRLPR"
     gene            complement(5556..>5962)
                     /locus_tag="VIG70_00540"
     CDS             complement(5556..>5962)
                     /locus_tag="VIG70_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY8249067.1"
                     /translation="PASASIEQAQRTIAWAEHLVVVFPLWLGDMPAVLKGFLEQVLRP
                     GFAIGKAAPGRMAKGLLKGRSARIVVTMGMPAFFYAIYYRAHSLKSLKRNILEFCGVS
                     PVRTTVIGMVEGRKEAREDWLTRLSVLGVQGS"
BASE COUNT          881 a         2123 c         2107 g          851 t
ORIGIN      
        1 ggcgaggtac accagcgcgg gcacgaagaa cgcgatcgcc acgttcgcct gcagcgtgac
       61 ctccatcgcc gccatcgcgg cggtggcgag cgcgctgccg gccaggccga ccagcagcca
      121 gggcagccgg tggcgcacgc gccgcagcgg cggatcctcg atcgcgtggc gcgcctgcgc
      181 cgcctcgcgc tggatgccgg cgagccggtg caggtcctcg acgtgctcgc ggcgcagcac
      241 ctgcagcagc gcctgcgcgg gcatcacgcc gagcgcgtgg ccttcgtcgt cgaccaccgg
      301 cagggcgtcg acgccgtggt gcaaagcgag tgaggccgcg tgctcctggt cggtgtcggc
      361 cgtgacacgc ggagacttgc tgtccaggat ttccttgaga ggcaccgcgg cgggagcggc
      421 gaacagcctg ccgagcgaaa ccacgccttt gagcttgcca tgaccgttca ccaccaggac
      481 gagctcgacg ctcaccggct tgtcgtgcgc gaggcgcgcg agcaccgcgc cggcggtctc
      541 gtccggccgc gcgcgcagca ccgacgcagc cacgtggccg cgcaccgcct cgtgatgctt
      601 catggcgcgc cagtatcgcc gccgcgccgg gaatgccttt gacctgggtc aagcgcgccc
      661 ggcaggcgaa accgacaatc gactggagca ccatggccga gtatcccgcc catctcgccg
      721 tgcgccgccg gctcgccgac ggccgcagcg ttgtcatccg cccgatccgg ccgcaggacg
      781 aggtgcgcca gcgcgagttc ctcgaccgcc tctccggcga cacgcggcgc cggcgtttca
      841 tgaagcgtgc cgacgcagtg gacggcgggc tcgcccattt ccattccgac atcgattacg
      901 accgccacat gggattcgta tgcgaatcgg gcgataccct ggtcggcgac gcacgctacg
      961 tagccaacgc ggaccggcgc agctgcgagt tcggcgtcgt ggtcgccgac gactggcacc
     1021 acaccgggat cgcgcagctc ctgatggacg cgctgatccg cgcggcgcgc gcgcgcggct
     1081 tcgagaccat ggagggcatc gtcctggagg acaatcggga catgctcgac ttcgtccggg
     1141 agctcggctt cgagcgcgct ccggccgccg agcagccggc catcgtcagg atcgtgaaga
     1201 agctttgagt acgcccgtcg tgttcgcggc ccggcctcca gtcgcgctcg ccgcttgacg
     1261 caggtcaaac ccagccgcga tcctcgagca acgcaacgat gcgcgccgcg ctttccggcg
     1321 gcgtgccctg atcgccgcgc accacgagct cggcggcctg cggcggctcg taggggatac
     1381 tcatgccctt gcggtaaagc cccttcgggt cgcgcgccct gcacacctcg agcggcgtgt
     1441 cgacgaagac ctcggcgaag cgcgcgatgc gcttgcgtgc cgcgtcacgg tatgcgcgcc
     1501 ggttcgcggt cgcgtcgaat accacggctt tctgcttctc caccagcagg aagccgattt
     1561 cgccgagcgt ggcatagaag aaatcgcgat ccgcatcgtc gtagcgcgga aagggcgtga
     1621 gctgcgtgcg catcacgtcg gattcgagca ccgaaacgtc tacgcggcgc tcgtgcagca
     1681 gccggacgag ctcgcgcgcg atggcgctct tgcccgagcc cgaatggccg gtcagccaga
     1741 ccgcgaagct catgcgcagt aggcgttcgc gcttgccggc tcgaagcgct cttctgccag
     1801 gacggcatgg atgaaattga agagcttgcg ccgcacaccg gtgtcgaggg cggggtacca
     1861 gagcgggttt gccatcacca gcccccggaa agcgaggaag ggggcggcca cctcgaggat
     1921 ctcgcggtcg cccgagcgct cgaggtagcg gtcccagaag cgccgccaca gccgctcgaa
     1981 gtcgccctca agccttccgg aacgctgcag cgagaagaac aggtagttca gcgtgaggca
     2041 agtcacgtcg tcggcggcgt cgccccactc gccgcgcgag cggtcgagca cgctgaagtc
     2101 cgtgccccgg cggaacatga tgttccaggg atgaaagtcg ccgtgcacct ggcgcagccg
     2161 gcggctgcgc gccttcagct tccagcgcca ttccacgcag ctcttttcaa tggattcgag
     2221 aaccgagttc gggaccgggc catccccggg ataactgtcg gccacgccga agatgcactc
     2281 gccgtggccg agcagctcgc gaatgcggcg cacgtacagg ccgggatccg gtccggcgac
     2341 gcggtgaatc tccaccaggt aatcgcacaa tgcgtcggcg cgggtttcat ccagcggcga
     2401 aagcgctttc ccggcccgca gcttcaggag atcgtccgcg tactcggcgc cctcgacgaa
     2461 ctcggcgagc atgaacagct cctcggcatt gccgagctgc accagcgcgc cgctcgagcg
     2521 caccgcgccg acgtcgagcg acaccacgtg gcgcggcagc ttgccgaagg cgccgtgcgc
     2581 ccacagcagc gactgggcgc ggtcggccat gtgctcgtgg ccgaacgggc cggggcgcac
     2641 ggtttcgacc accgcgcggc gcagcacgcc gtcgagcgtg tagtcgacgc gcagcggaat
     2701 gccgtagccg tagcccttgt cgccctggcg gccgccgccg agcggggtga cgcgcacgcc
     2761 gctcaccgcg cggccgtgca ctgcgccgag ataggccgcc agcgcctgcg gcgtgaacgc
     2821 agaaggcatg ctcgagctta ccggagcctg cggcgcaggc ggtttgatct cggtcaaacg
     2881 cgcccgccgc ccgcccggct acgctggggc gatgaaaccc tggcaacgcg accaattgcg
     2941 ccgcgcgctc gagcagcgcc gcgaaaccct tctcgaagag ctcaagcgcg atgccgcacg
     3001 gctgcgcgaa gagcgttctg gagacatcgg cgacatcggc gacgaatcgg tcgccgacct
     3061 gctggtcgac ctcgaccagg ccgacctgtc gcgcgacgcc ggagagctgc gcgagatcga
     3121 ggccgcgcgc cggcgcctcg acggcgggac ctacggcatc tgcgccgact gcggcgccgc
     3181 gatcgatttc gagcgcctgc gcgccgagcc cgccgccgcg cgctgcgtcg agtgccaggc
     3241 gcggcacgag aagacctacc ggcgatagaa gtggtagcgg ttgccgccgg cggccttggc
     3301 ggcgtacatc gcaaggtccg cctgcttgcg cagcgcgtcg gcgtcctgcg cgtcgcgcgg
     3361 gtagccgctg gcgccgacgc taactgtggc gcggatgttg gcgccgtcgt ggcgcaccgg
     3421 cgccgcgttc gcggccacga tcttggcgag caccggcccg gcctgcgctt cggtcgcatc
     3481 gaggaggatg accgcgagct cgtcgccgcc gatgcgcgcc acggtgtcgg cctcgcgcac
     3541 gcagccgcgg gtgcgcgccg ccaccgcctg cagcaccgtg tcgcccgcca tgtgtccgca
     3601 gcggttgttc gtgtcgcgga agcggtcgat gtcgaggatc gcgagcgtga gctgctcctc
     3661 ccgccgcgcg gcgtggcgca gcgcctggcc cagccgatcg aggaacaggc tgcggttcgg
     3721 caggtcggtc aggctgtcgt gcgtcgcggc gtgcagcagc cggtcgaact cgcgcgcgcg
     3781 ctcggtggcg tcgcgcccga acgagacgaa gtgcgtggcg cggccgtcgg cgtcgaacag
     3841 cgggcggatg atcttgtcct cgtagtacgg ctcgccgctt ttcttgcggt tggcgaacag
     3901 cgcgcggaat tcccgcccgg cgaggagcgc ggaccagagc tcgcggtaga aatcggcctc
     3961 gtgcatcccg gacttgagca gtgccggcgt gcgccccgcg agctcgctcg aggcatagcc
     4021 ggtcatgcgc tcgaacgccg ggttcgcgta gacgatgcgg ccggcggtat cggtgaccag
     4081 caccgcgtcc gggcactgct cgatgtagcg ccggaatgcc ggctccatga ccgaaaacta
     4141 atcgggaccg ggcgaagccc gttgatcctc gtcaaagccg cggcgcaagc gagccggtac
     4201 cgtggccgga tgagagcgct cctcgccctc ctcgtcctcc tcgcggcccc gcccacggcc
     4261 gcccaggcgc cggacttcac cgccctggtg agggaaggcg ccgctgtcgt caacctgagc
     4321 ggcacgctgg gcggggtgct gcccgacctg ccgctcgccg aagaagaagc ggaagacacc
     4381 gggatcctgc gcgacttcct gcgccgcgag tacggcccgg caccggaaac gcgctcgctc
     4441 ggctccggct tcgtgatcga cccttccggc tacatcatca ccaacgcgca cctggtcgcc
     4501 agcactgccg agatcagcgt gcgcctcgcc gaccgccgcg agttcgaggc cagggtcctc
     4561 ggcctggacc cggcgagcga catcgcgctc ctcaagatcg aagcgagcgg gctcgcgacg
     4621 gtgcgcatcg gcgatccggc gaagctgcgt ccgggggaat gggtcgcggc gatcggctcg
     4681 ccgttcggcc tggagcgcag cgtcacggcg ggcatcgtga gcgcgctcgg ccgcacgctt
     4741 ccgcaggaaa gcttcgtggc gttcatccag accgacgtgg cggtcaaccc gggtaactcg
     4801 ggcggcccgc tcttcaacct gcggggcgaa gtggtgggtg tgaactcggg gttctacagc
     4861 gacaccggcg gctacatcgg gctttcgttc gccatcccga tcgacgtcgc gatggaggtg
     4921 gccgccgagt tgcgcgcgta cggcaaggtg acgcgcggcc gcatcggcgt gcgcctgcag
     4981 gagttgaccg ccgagctcgc ccgggcgctc aagctgccgc gcgccgccgg cgccctcgtg
     5041 gtcgagacgt tcccgggcta cgcggcggcg cgcgccggcg tgcgacccgg cgacgtcatc
     5101 gtgcgcttcg acggccagcc ggtcgaggcc gaagccgagc tcgtgcggct caccgcgcgc
     5161 gcgaccccgg gcgcaacggt ggatgtcgag ttgctgcgct tcggcgcccc tcttctcctg
     5221 cggctcaagg tggacgccac acgcgtcgcg acgctgccgg cggcggtacg ccccgacggc
     5281 gagccgctcg gcctgcagct cgcgccgctc gaccgccggc agcgcgagcg catgcgcctc
     5341 gagagcggcc tcctcgtcga gcgcgcgcgc gccgcgtccc agcgcgccgg cctgatgcgc
     5401 ggcgacgtga tcgtgtcggt caacggcaag gcggtgtcga gcgcagaggc gttcgctgcc
     5461 gtgctgcagg ccgcgggcaa gggcggcacc gtggcgctcc tggtgcagcg cgacggcgtg
     5521 cggcagttcg tgccgctgcg gctgccgcgg tagcgctagc ttccctgcac gcccagcacg
     5581 ctgagccggg tcagccaatc ctcgcgcgct tccttgcggc cctcgaccat gccgatcacc
     5641 gtggtgcgca ccggcgagac gccgcagaac tcgaggatgt tgcgcttcag gctcttcagg
     5701 ctgtgggcgc ggtagtagat tgcgtagaag aacgccggca ttcccatcgt cacgacgatg
     5761 cgcgccgagc gccccttgag cagccccttc gccatgcgcc ccggcgccgc cttgccgatc
     5821 gcaaagccgg gccgcagcac ctgctcgagg aagcctttga ggaccgccgg catgtcgccg
     5881 agccagagcg ggaacacgac gacgagatgc tcggcccagg cgatggtgcg ctgggcctgc
     5941 tcgatcgacg ccgaagcggg ct
//
LOCUS       DATBSZ010000008         8744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_684230, whole genome shotgun sequence.
ACCESSION   DATBSZ010000008 DATBSZ010000000
VERSION     DATBSZ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8744
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684230"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /gene="ruvB"
                     /locus_tag="VIG70_00545"
     CDS             <1..727
                     /gene="ruvB"
                     /locus_tag="VIG70_00545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY8249068.1"
                     /translation="NLEPRDVLFIDEIHRLSPVVEEILYPALEDFQLDIMIGEGPAAR
                     SIKLDLPPFTLAGATTRAGMLTNPLRDRFGIVARLEFYSHQDLAKIVQRSARLLKVAL
                     EDEGASEIAKRARGTPRVANRLLRRVRDYAQVKGDGTVSKSVAEAALKMLDVDVLGLD
                     VMDRKLLLAVIEKFSGGPVGLENLAHAVGEEADTIEDVLEPYLIQQGYLQRTPRGRIA
                     TLSAYRHFGIVAPTAVKDLLSDT"
     gene            739..1110
                     /gene="ybgC"
                     /locus_tag="VIG70_00550"
     CDS             739..1110
                     /gene="ybgC"
                     /locus_tag="VIG70_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEY8249069.1"
                     /translation="MYYQDTDAGGVVYHAQYLSFMERARSELLNEAGIDLARFVEEHG
                     TLFMVHRIDVKYHLPARLNDLLSVSAEVVKMGRASFVFRQRVERGAELLVEADVTLAL
                     VDRERMKPARMPAELEQALSK"
     gene            1107..1793
                     /gene="tolQ"
                     /locus_tag="VIG70_00555"
     CDS             1107..1793
                     /gene="tolQ"
                     /locus_tag="VIG70_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEY8249070.1"
                     /translation="MTVTQDLDIWTLILNASIVVKVVMLLLAAVSFMSWMFIFQKWFA
                     IRRAGVQTEKFEREFWSGNDLNALYQGAVNQRHTIGSLERIFEAGFREFAKLRSGQRA
                     GTDASNMVDGARRAMRATFQREMDFLERHLAFLASTGSVSPYVGLFGTVWGIMHAFRS
                     LANVQQATLAQVAPGIAEALVATAIGLFAAIPAVVAYNRYSHDIERLANRFESFMEEF
                     SNILHRQAVR"
     gene            1793..2215
                     /locus_tag="VIG70_00560"
     CDS             1793..2215
                     /locus_tag="VIG70_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8249071.1"
                     /translation="MALRKKRGPVHEINVVPYVDVMLVLLVIFMVTAPLVTPAVIDLP
                     TVDKASAPRLVPLEIFVKSDQGLVVRQRDARGGVVNEQSLSRAQLPGFIKSLKNKGDL
                     SVLIGGDKNARYESVLQVMDELRKHGVQKVGLQVKTSS"
     gene            2257..2973
                     /locus_tag="VIG70_00565"
     CDS             2257..2973
                     /locus_tag="VIG70_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY8249072.1"
                     /translation="MGRSFVLSALVHIVLAAVLVLGVRWQVSAPDVVEVDLVDAPPPP
                     PPAAEVPKPLPKVEPSPVVKPEPPKVVKPQIAIPEKPKPKPKPKPEVKPKADPNFDRT
                     MREQMAREQKVFEQQRQERELRELLARKQAEEARAAAAAKTKALNEYIARIQAKVKSG
                     WILPQDIQGNPEAIFIVVQLPTGEVLPDTGVRLVKSSGNPAYDAAVERAILKASPLPL
                     PPPGVPFSRELKLTFRPHDK"
     gene            3029..4276
                     /gene="tolB"
                     /locus_tag="VIG70_00570"
     CDS             3029..4276
                     /gene="tolB"
                     /locus_tag="VIG70_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEY8249073.1"
                     /translation="MFLVAASQAAAQLSIEITGAGAQRTPIAIVPFAGEAAVGSSLTA
                     IVRADLERSGLFRGLEVPPMSPTPNENTPVSYGEWRSRLADALVLGSVGKSADGRFEV
                     RFRVYDIVKQAPLGAIAYLFTQDNARAAAHRIADFVYEKLTGEKGVFSTQIAYVVKRG
                     STYELQIADADGERAQYLLRSNEPIISPVWSPEGRRIAYVSFESKKPIVYVHHLSENK
                     RQVVASFKGSNSAPAWSPDGKTIAVVLSVEGGSQIYLLNSDGSGPRRRISNSNSIDTE
                     PRYSTDGKLLYFTSDRGGSPQIYRMSSTGGEPQRVTFKGSYNVSPRPSPDGRVLAYVT
                     REAGKFQVALLDLANQQVQVITDSDRDESPSFAPNSKMILLATVRGGRGVLSAVSSDG
                     RIRQNLPYSGGDVREPAWGPFNE"
     gene            4291..4857
                     /gene="pal"
                     /locus_tag="VIG70_00575"
     CDS             4291..4857
                     /gene="pal"
                     /locus_tag="VIG70_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY8249074.1"
                     /translation="MRAFFILLAFLVAASGCQTTPEDQAAASASVEDRTKPGAGKPGA
                     QAQPVERPKVAAVDVTSGRKDPASALKDPANILSKRSIYYELDKFDVRDEYRGLVEAH
                     AKYLRENPKTRVLIQGNTDERGSREYNVGLGQRRSDGVKRMLILLGAREDQIESVSLG
                     EEKPQAEGQSETAWSKNRRSDILYSGEF"
     gene            4857..5675
                     /gene="ybgF"
                     /locus_tag="VIG70_00580"
     CDS             4857..5675
                     /gene="ybgF"
                     /locus_tag="VIG70_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY8249075.1"
                     /translation="MLRALILGFLAATSAGSAWAQLFDDNEARRRIELLRQQVETQRQ
                     TTEARLSKAETAAQEASERAADRGALLEISNQIDVLRGEIAKMRGQLEVLNHQGETAE
                     ARQKQLYLDIDTRLRKLEQAREQQAAVPEKPPAPAPSVADAEPTPAEAKAYQAALDQF
                     KLGNYTLAVAAMQGFLVTYPSSSLAANAQYWIGMAHSGQRDYKSAIAAQRKLLVQWPD
                     SPKAPDAMLSIASAQETMGDRTSARKTLEELIAKYPGSSSATSAKQRLAAFSKR"
     gene            5672..6751
                     /locus_tag="VIG70_00585"
     CDS             5672..6751
                     /locus_tag="VIG70_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEY8249076.1"
                     /translation="MNETYLYVVGGAFLLAFIFGAVGNKTHFCTMGAVSDWVNMGDTS
                     RLRMWLLAIAVAILGSSALHLAGVVDLSKSIYPGPNFTWLSYIVGGFLFGVGMTLGSG
                     CGSKTLIRVGTGNLKSLVVYIFLGIAAYMTLRGLLGAFRVGVLEKATITLPQGQDLPA
                     LLSLNRAILAALIGGGLLAYIYSSKQFRSTFDYTLGGVVTGLVVVGGWYVSGHIGHVA
                     EDPDTLQEAFVATNTGRMESFSFVAPFAFTLEYLMLWTDKSKIITYGIASAAGVIAGS
                     AAYALATRSFRWESFRDAEDTANHIVGGILMGFGGITALGCTIGQGISGFSTLALGSI
                     MTFVAIVAGSAATMKYQVWRIERQG"
     gene            6794..6869
                     /locus_tag="VIG70_00590"
     tRNA            6794..6869
                     /locus_tag="VIG70_00590"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6827..6829,aa:Lys,seq:ttt)
     gene            complement(6939..>8744)
                     /locus_tag="VIG70_00595"
     CDS             complement(6939..>8744)
                     /locus_tag="VIG70_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249077.1"
                     /translation="VGVNQVVWDPATLTLHVESDEVLRQTARYAVIVTNGILDTSGRP
                     VQASDAFDDFRHDLNFGQAKSKELNQYRKALIKTLAALKRWGVSKRDVVALSVFTTQS
                     ATATLENIRDYLKSSPPPALANFAIGPGGSRAVFAVSSIQSITFRQQTGTSTFVNAPV
                     PLAVLNAFGAGAVSTIAWGTFVSPQFIDSNAVMTTHGTLEMPSQFSNATLEFVLFLPS
                     GTQPASGWPVAFLGHGGTTNIHTAAVWNFASSLAAHGFAAIAINAIGRGFGPLGTLSI
                     TKTDGTTVQITAGGRTIDQNGDGVIANQEGAAATAPNLIIGQRDAVRQTAIDYIQLVR
                     VIQAGIDVDGDGSADIDASRMVFLGNSFAVGYETCFLAVEPDVVTAAVGSPGGLPGRP
                     DLLSMRPSGRTMVGAALAARTPSLLNEAHGLTSIGGIPATAPFYNENIPLRDLPPVTN
                     DIPGAMAIQEHFDRIEWVQASSDAATHARHLESEPLAGVPRKSILVLYAKGDMTAPNP
                     RTTALVRAGVLREKTIFYRNDIALAEDPTVPKDPHTFLQPAASPGITGQVARGGQTTI
                     AQFLVSGGTTINHPEPARFFEFPIVVMPEDYSYIP"
BASE COUNT         1526 a         2859 c         2908 g         1451 t
ORIGIN      
        1 gaacctggag ccccgggacg tattgttcat cgacgagatc catcgcctct cgccggtggt
       61 ggaggagatc ctgtacccgg cgctggagga cttccagctc gacatcatga tcggcgaggg
      121 gccggcggcg cgctcgatca agctcgacct gccgccgttc accctcgcgg gcgccaccac
      181 ccgcgccggc atgctgacca acccgctgcg cgaccgcttc ggcatcgtcg cgcggctgga
      241 gttttactct catcaggacc tggccaaaat cgtgcagcgc tccgcacgct tgctgaaggt
      301 ggcgctggag gatgaaggcg cgagcgagat tgcgaagcgc gcgcgcggca cgccgcgcgt
      361 agcgaaccgg ctgctgaggc gggtgcgcga ttacgcgcag gtgaagggcg acggcacggt
      421 gtcgaagagc gtggccgaag cggccctcaa gatgctcgac gtcgacgtgc tcggcctcga
      481 cgtgatggac cgcaagctgc tgctcgcagt gatcgagaag ttcagcggcg ggccggtggg
      541 tctcgagaac ctggcgcacg cggtgggcga ggaggccgac accatcgagg acgtgctcga
      601 gccctacctc atccagcagg gctacctgca gcgcaccccg cgcggacgga tcgcgacgct
      661 ctcggcgtac cgccatttcg gcatcgtggc gccgacggcg gtgaaggact tgttgtcaga
      721 tacctgatcc ccgtccgggt gtactaccag gacaccgacg cgggcggtgt ggtgtaccac
      781 gcccagtacc tctccttcat ggagcgcgcc cgcagcgagc tcctgaacga agccggcatc
      841 gacctggcgc gcttcgtcga ggaacacgga acgctcttta tggtccatcg catcgacgtg
      901 aaatatcatc tgccggcgcg gctcaacgac ctgctctcgg tgagtgccga agtggttaaa
      961 atgggccgcg cgagcttcgt gttccggcag cgcgtggaac gcggcgccga gctgctggtc
     1021 gaagccgacg tcacgctcgc gctggtcgac cgcgaacgga tgaagcccgc gcgcatgccc
     1081 gcagaactcg aacaggcgct ctcgaaatga cggtcacgca agacctcgac atctggacgc
     1141 tgatcctcaa cgccagcatc gtagtcaagg tggtgatgct gctgctcgcc gcggtctcgt
     1201 tcatgtcctg gatgttcatc ttccagaagt ggttcgccat ccgccgcgcc ggcgtgcaga
     1261 ccgagaagtt cgagcgcgag ttctggagcg gcaacgatct caacgcgctc taccagggcg
     1321 cggtcaacca gcgccacacc atcggcagcc tggagcgcat tttcgaggcc ggcttccgcg
     1381 agttcgccaa gctgaggagc ggccagcgcg ccggcaccga cgcgagcaac atggtcgacg
     1441 gcgcgcgccg cgcgatgcgc gccaccttcc agcgcgagat ggacttcctc gagcgccacc
     1501 tcgcgttcct cgcctcgacc ggttcggtga gcccctacgt gggcctgttc ggcacggtgt
     1561 ggggcatcat gcacgcgttc cgaagcctgg cgaacgtcca gcaggcgacg ctcgcgcagg
     1621 tggcgccggg catcgcggag gcgctggtcg ctaccgcgat cggcctcttt gcagcgatcc
     1681 ccgcggtcgt ggcgtacaac cgctactcgc acgacatcga acgcctcgcc aatcgcttcg
     1741 agagcttcat ggaggagttc tcgaacatcc tccatcggca agcagtgagg taatggcctt
     1801 acgcaaaaaa cgagggccgg tgcacgagat caacgtcgtg ccgtacgtcg acgtgatgct
     1861 ggtgctgctg gtgatcttca tggtgaccgc gccgctcgtc accccggcgg tcatcgacct
     1921 ccccaccgtc gacaaggcga gcgcgccgcg cctcgtgccg ctcgagatct tcgtgaagag
     1981 cgaccagggc ctggtggtgc gccagcgcga tgcgcgcggc ggcgtggtga acgagcagtc
     2041 gctcagccgg gctcagcttc cggggtttat caagagtctt aaaaataaag gcgacttgtc
     2101 agtgctgatc ggcggcgaca agaacgcgcg ctacgagtcg gtgctgcaag tcatggatga
     2161 gctgcgcaag cacggcgtgc agaaagtcgg cttgcaggtg aagacctcct cgtgagcacc
     2221 ctgaccttgg ggctctggca gcgcaatgag cattggatgg gccgatcgtt cgtgctttcg
     2281 gcgctggtgc acatcgtgct cgcggcggtg ctggtgctcg gcgtgcgctg gcaggtcagt
     2341 gcgccggacg tcgtggaggt ggacctggtc gacgcgccgc cgcccccgcc gccggccgcg
     2401 gaagtgccca agccgctgcc caaggtcgag ccgtcgccgg tcgtgaagcc cgagccgccg
     2461 aaagtggtga agccgcagat cgcgattccg gagaagccca agccgaaacc caagcccaag
     2521 ccggaggtga agcccaaggc cgacccgaac ttcgaccgga cgatgcgcga gcagatggcg
     2581 cgggagcaga aggtgttcga gcagcagcgc caggagcgcg agctgcgcga gctgctcgcg
     2641 cgcaagcaag ccgaggaggc gcgggccgcc gccgcggcca agaccaaggc gttgaacgag
     2701 tacatcgcgc gtatccaggc caaggtgaag tcgggctgga tcctgccgca ggacatccag
     2761 ggcaaccccg aggcgatctt catcgtcgtt cagctgccga cgggagaggt gctgcccgat
     2821 accggtgtca ggctagtcaa atcgagtggc aatcccgcct acgacgccgc ggtcgagcgg
     2881 gcgatcctga aggcctcgcc gctgccgttg cccccgcccg gcgtgccgtt ttcgcgggag
     2941 ttgaaactta ctttccgccc gcatgacaaa taggatgcgc ttacaagaac atcgggagaa
     3001 aaccagcttg cgccttcttt ttggactagt gttcctggta gctgcgtcac aagcggcggc
     3061 ccagctttcg atcgagatca ccggcgccgg ggcgcagcgc accccgatcg ccatcgtgcc
     3121 gttcgccggc gaggccgcgg tcggctcgag cctgaccgcg atcgtgcgcg ccgacctgga
     3181 gaggagcggc ctgttccgcg gcctcgaggt gccgccgatg tctccgaccc cgaacgagaa
     3241 taccccggtg agctacggcg aatggcgctc gcgcctcgcc gacgcgctgg tgctgggctc
     3301 ggtggggaag agtgccgacg gccgcttcga ggtccggttc cgcgtctacg acatcgtgaa
     3361 gcaggcgccg ctcggggcga tcgcgtatct cttcacccag gacaacgcgc gcgcggcggc
     3421 gcaccgcatc gccgacttcg tctacgagaa gctgaccggc gagaagggag tcttctccac
     3481 ccagatcgcc tacgtggtga agcgcggcag cacctacgag ctgcagatcg ccgacgccga
     3541 cggcgagcgc gcacagtacc tgctgcgctc gaacgagccg atcatctcgc cggtgtggtc
     3601 gcccgagggg cggcgcatcg cctacgtgtc gttcgaatcg aaaaaaccga tcgtctacgt
     3661 gcaccacctg tccgagaaca agcgccaggt ggtggcgagc ttcaaggggt cgaactcggc
     3721 gccggcctgg tcgcccgacg gcaagaccat cgccgtggtg ctctcggtcg agggcggctc
     3781 gcagatctac ctcctcaact cggacggcag cggcccgcgg cggcgcatca gcaactccaa
     3841 ctcgatcgac accgagccgc gctactcgac cgacggcaag ttgctctact tcacctccga
     3901 ccgcggcggc agcccgcaga tctaccgcat gagctcgacc ggcggcgagc cgcagcgcgt
     3961 gactttcaag ggcagctata atgtctcgcc gcgccccagt cccgacggcc gcgtgctcgc
     4021 ctacgtgacg cgcgaagccg ggaagttcca ggtggcgctg ctcgatttgg cgaatcaaca
     4081 ggtgcaggtc attaccgaca gcgaccgcga cgagtcgccg agcttcgccc ccaacagcaa
     4141 gatgatcctg ctcgccacgg tgaggggcgg ccgcggcgtg ctgtccgccg tgtcctccga
     4201 cggtcgcatc cggcagaacc tgccatactc cggcggcgac gttcgcgagc cggcgtgggg
     4261 gccgttcaac gaatgaaagg agtgaaaccc atgcgcgcgt ttttcatcct gttggctttc
     4321 ttggttgcgg cgtcgggctg ccagaccact cccgaagatc aggcggcggc atcagcgagc
     4381 gtcgaggatc gcaccaagcc cggcgccggc aagcccggcg cccaggcgca gccggtcgag
     4441 cggccgaagg tcgccgcggt cgacgtcacc tccgggcgca aggatccggc gagcgccctg
     4501 aaagacccgg cgaacatcct ctccaagcgc tcgatctact acgagctgga caagttcgac
     4561 gtccgggacg aataccgcgg cctggtcgag gcgcatgcca agtacctgcg cgagaacccc
     4621 aagacccggg tgctgatcca gggcaacacc gacgagcgcg gtagccgcga gtacaacgtc
     4681 gggctcggcc agcgccgctc cgacggggtg aagcgcatgc tgatcctgct gggcgcgcgc
     4741 gaagaccaga tcgagtcggt gagcctcggc gaggagaagc cgcaagccga aggccagagc
     4801 gagaccgcgt ggtccaagaa ccggcgcagc gacatcctct atagcggcga gttctgatgc
     4861 ttcgggctct gatactgggt tttctggccg caacctcggc cggctctgcg tgggcccaac
     4921 tgttcgacga caacgaagcg cgccgccgca tcgagctctt gcgccagcag gtcgagacgc
     4981 aacgccagac gaccgaggcg cgcctgagca aggccgagac ggccgcccag gaggcgtcgg
     5041 agcgggccgc ggaccgcggc gcgctgctcg agatctcgaa ccagatcgac gtcctgcgcg
     5101 gcgagatcgc gaaaatgcgc ggccagctcg aggtcctcaa ccatcagggc gagactgcgg
     5161 aagcgcgcca gaagcagctc tatctcgaca tcgacacgcg cctgcgcaag ctcgagcagg
     5221 cgcgcgagca gcaggccgcg gtgcccgaga agccgcccgc acccgcgccg tccgtcgccg
     5281 atgccgagcc cactcccgcc gaggcaaagg cgtatcaggc cgcgctcgac cagttcaagc
     5341 tcggcaacta cacgctcgcc gtcgcggcca tgcaaggctt cctcgtcacc tacccgagca
     5401 gctcgctcgc ggcgaacgcg cagtactgga tcggcatggc ccattccggc cagcgcgact
     5461 acaagagcgc catcgctgcc cagcgcaagc tgctggtgca atggcccgac agcccgaaag
     5521 cgcccgacgc gatgctgtcg atcgcgagcg cgcaggagac catgggcgac cggacctctg
     5581 cgcgcaagac gcttgaagag ctgatcgcca agtatccggg gagcagctcc gcgacgagcg
     5641 ccaagcagcg cctcgccgcc ttttccaagc gttgaacgaa acatatctgt acgtagtcgg
     5701 gggagcgttc ctcctcgcgt tcatcttcgg cgcggtgggc aacaagaccc acttctgcac
     5761 catgggtgcg gtctccgact gggtcaacat gggcgacacc agccgcctgc gcatgtggct
     5821 cctcgccatc gcggtcgcca tcctcggctc gagcgcgctg cacctcgccg gcgtggtcga
     5881 cctgtcgaag tcgatctatc ccggcccgaa cttcacctgg ctctcctaca tcgtcggtgg
     5941 gttcctgttc ggcgtcggca tgacgctcgg ctcgggctgc ggctccaaga cgctcattcg
     6001 cgtcggcacg ggcaatctga aatcgctggt ggtctacatc ttcctcggca tcgcggcgta
     6061 catgacactg cgcgggctac tgggcgcgtt ccgcgtcggc gtgctggaga aagcgaccat
     6121 cacgctcccg cagggccagg atcttccagc cctcctctct ctcaatcgag caatccttgc
     6181 cgcgctgatc ggcggcgggc tgctggcgta catctactcc agcaagcagt tccgttcgac
     6241 cttcgactac accctgggcg gagtggtgac gggcctcgtc gtggtcggcg gctggtatgt
     6301 gagcgggcat atcggccacg tcgccgagga cccggacacg ctgcaggaag ccttcgtcgc
     6361 caccaacacc gggcgcatgg aatccttttc ctttgtggcg ccgttcgcct ttaccctcga
     6421 atatctgatg ctgtggactg acaagtcgaa gatcattacc tacggcatcg cctccgccgc
     6481 cggcgtgatc gccggctcgg cggcctatgc gctcgccacg cgcagcttcc ggtgggaaag
     6541 cttccgcgat gccgaggaca cggcgaacca catcgtcggc ggcatcctga tgggtttcgg
     6601 cggcatcacc gcgctcggct gcaccatcgg gcaggggatc tcggggttct ccacgctcgc
     6661 gctcggctcg atcatgacct tcgttgcgat cgtcgcgggc tccgccgcga ccatgaagta
     6721 ccaggtctgg cggatcgagc gtcaaggctg ataaaattcc gccctttccg cagcaaatcc
     6781 gcgtcgtaag cgtgggtcgt tagctcagct ggtagagcag cggactttta atccgttggt
     6841 cggcggttcg aatccgccac gacctaccaa atcttcttcc caagagtagc cgcccgcgtc
     6901 caaacgtctc ggcgctgagt tgtcagcccc cacggcgact acgggatgta ggagtagtcc
     6961 tccggcatga ccacgatcgg aaattcgaag aaccgggccg gttcgggatg gttgatggtg
     7021 gtcccgcccg aaacaaggaa ctgggcgatt gtcgtctgac cgccgcgggc gacctggccg
     7081 gtgatgccgg ggctcgcggc gggctgcagg aaggtatggg gatccttggg aaccgtggga
     7141 tcctcggcaa gggcgatgtc gttgcggtag aagatggtct tttcgcgcag cacgccggct
     7201 cgtactaagg cggtagtcct gggattggga gccgtcatgt cgcccttggc gtagaggacg
     7261 agaatggact tccttggaac tcccgccagg ggctcggact ccaggtgccg agcgtgggtg
     7321 gccgcgtcac tgctcgcctg cacccactcg atgcggtcga agtgttcctg gattgccatc
     7381 gcaccgggga tgtcgttggt cacgggcggg agatcgcgca gcgggatgtt ctcgttgtag
     7441 aacggcgccg tcgcagggat gccgccgata gaggtcagac catgggcctc gttcaggagc
     7501 gacggcgtgc gcgcggcgag cgcggcgccg accatcgtgc gtcccgaagg ccgcatgctg
     7561 agcaggtcgg ggcggccggg aagccccccg ggcgagccaa ccgcggcggt gacgacgtcg
     7621 ggctcgaccg ccaggaagca ggtctcgtag ccgaccgcga acgagttgcc gagaaacacc
     7681 atcctcgagg cgtcgatgtc ggctgagccg tctccgtcga cgtcgattcc agcctggatg
     7741 acgcggacga gctggatgta gtcgatagcg gtctggcgta ccgcgtctcg ctggccgatg
     7801 atcaggttgg gcgccgtcgc agctgcgcct tcctggttcg caatcacgcc gtcgccgttc
     7861 tgatcgatcg tccgtccgcc agccgtgatc tggaccgtcg tcccgtcggt ctttgtgata
     7921 ctcagtgtgc ccaggggtcc aaagccacgg ccgatggcat tgatggcgat cgcggcaaat
     7981 ccgtgggccg cgagcgaact ggcaaagttc cagaccgcgg cagtgtggat attggtggtg
     8041 ccgccgtgcc cgaggaaggc gaccggccac ccagacgccg gctgggtgcc gctcggcagg
     8101 aacagcacga actcgagagt tgcgttgctg aactggctgg gcatctcgag cgtcccgtgg
     8161 gtggtcatca ccgcattgct gtcaatgaac tgcggcgaaa caaaggtccc ccatgcgatg
     8221 gtgctcacgg ctccggcgcc gaaggcgttc agaacagcga gcggcactgg cgcgttgacg
     8281 aacgtgctcg tcccggtctg ctgtctgaag gtgatgctct ggatcgagga cacggcgaac
     8341 accgcgcgtg acccgcccgg tccgatcgca aagttggcca gagccggggg cggggagctc
     8401 ttcaaatagt cgcgaatgtt ctcgagcgtc gcggtggcgc tctgggtcgt gaagacgcta
     8461 agcgccacca cgtctcgctt ggagacgccc caccgtttga gggccgccag agtcttgatc
     8521 agcgcttttc ggtactggtt gagctccttg ctcttggcct gtccaaaatt caggtcgtgc
     8581 ctgaaatcgt cgaaggcatc cgatgcttgc accggccgcc ccgacgtatc aaggatgccg
     8641 ttagtgacga tcaccgcgta gcgggcggtt tggcggagta cttcatcgga ctcgacgtgc
     8701 agggtgagtg tcgccggatc ccagaccacc tgattgactc caac
//
LOCUS       DATBSZ010000009         9302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2118699, whole genome shotgun sequence.
ACCESSION   DATBSZ010000009 DATBSZ010000000
VERSION     DATBSZ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9302
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2118699"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VIG70_00600"
     CDS             <1..243
                     /locus_tag="VIG70_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8249078.1"
                     /translation="EQLVAAPDNPARQAEAREALTRLLQAIESLPAAQREAFLLHEEA
                     GLSVAEIAAATGTNDEAAKSRLRYAMAKLKAAVDDG"
     gene            236..1015
                     /locus_tag="VIG70_00605"
     CDS             236..1015
                     /locus_tag="VIG70_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249079.1"
                     /translation="MADERKISQRYRELPREEPPHHLDQAILAAARRAAEIRPAPLVV
                     PSGRRRWYFPLAAAAVIVLAVAVTMHVEREQPDVEMAEAPVAASPPPAPLAREEQAAP
                     AQAPAKREAFTPDPKPAPSRDRAPGAELPADLRKAQKEAQNAPSSPAPPPAASAPVPA
                     QDAVSGARESARADAGAKPSAPMRQESRVRASSAPGAAPAVAGALGAFAYTSPEQWLQ
                     GIDDLKRQRRHDEAEKQLAEFRKRYPDYRIPEAILEKFEKR"
     gene            complement(1016..1981)
                     /locus_tag="VIG70_00610"
     CDS             complement(1016..1981)
                     /locus_tag="VIG70_00610"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8249080.1"
                     /translation="MTRCALVAGLWMFAAAALAQSKIATVGVLIAVSSAGHAPHLEQF
                     RKGLRDLGYVEGRNLRLEVRYAEAKIDRLPQLAADLAAIPVDVLVTGGSHVTRVARKA
                     TRDVPIVMAWAGDPVGGGLADSLARPGGRITGLTTLAPQLGGKRLEILREILPKMNDV
                     ALLWNPDVPERVIQFKETQAAAKRLRVTVHSFEARRKEEIDSALSSLAQKRPDAMIIL
                     SDALLDANLSTVAAFAAKHRLVAAHQQRRAAQLGMLLSYGASFSSLHYRAATYVDKIL
                     KGAKPGDLPIEQPTQFELVINLKTAKVLGIRVPQSVLVRADEVIQ"
     gene            complement(2019..2594)
                     /locus_tag="VIG70_00615"
     CDS             complement(2019..2594)
                     /locus_tag="VIG70_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8249081.1"
                     /translation="MAAPSKFAHIVYRTRRFEEMIGWYEKVFEAKVQYRNPVLAFLTY
                     DDEHHRFAFANLSVLDPAGGAGATRPDTGVDHVAYTYGSLADLLGTYTRLKAEGIVPY
                     WPIRHGPTLSLYYKDPDGNRLEFQVDCFATAEAANAYMRGDSFAANPIGVEFDPDELV
                     EQFRKGVPEARLLARPDGPGSPVPREHGIGA"
     gene            complement(2605..3333)
                     /locus_tag="VIG70_00620"
     CDS             complement(2605..3333)
                     /locus_tag="VIG70_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY8249082.1"
                     /translation="MISIRQVSKWYGSFQVLADCSTEVAKGEVVVVCGPSGSGKSTLI
                     KVVNALEPFQKGEITVDGVPVHDAKTDLPKLRSRIGMVFQNFELFPHLSVTRNLTLGQ
                     IKVLGRSEDEARAKGLKLLDRVGLIAHKDKYPGQLSGGQQQRVAIARALSMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELAREGMTMMVVTHEMGFARKVAHRVIFMDAGRIVEDA
                     KKDEFFGTPRSERAQLFLSKILHH"
     gene            complement(3330..4022)
                     /locus_tag="VIG70_00625"
     CDS             complement(3330..4022)
                     /locus_tag="VIG70_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY8249083.1"
                     /translation="MDAGFDYDVIQRSWVYLFKEGMTFTVTLTLLAMTGGIIFGTLLA
                     MMRLSAFKALSAVAGGYVNLMRSVPLVLVIFWFFFLVPYIGAWITGADRPVQVGAWLS
                     AVVTFTMFEAAYYCEIMRAGIQSIPRGQVWAGYALGLNYWQSMGQIVLPQAFRNMLPI
                     LLTQTIILFQDTSLVYVISATDFLGAASKVANRDYRLVEMYTFVAVVYFIISYSLSLL
                     VKRLQDRIAIIR"
     gene            complement(4023..4778)
                     /locus_tag="VIG70_00630"
     CDS             complement(4023..4778)
                     /locus_tag="VIG70_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY8249084.1"
                     /translation="MGYNWNWKVLLETEPGGTGTYLQYLIVGLGWTLVTALAAWIIAL
                     AIGALVGTLRTTPYPWVVRAGNLYVEIFRNIPLIVQMFLWFFVMPELLPQALGDWIKQ
                     MPPPWGSFIPAVLCLGIFTSVRIAEQVRAGINSLPRGQSMAGTALGLTLGQSYRYVVL
                     PQAFRIILPPLTSESMNIIKNSSVALTIGLLELTGRARAMQEFSFRVFEAFATATVIY
                     LLTNLVVVLLMRGLERKVRVPGLIGSHTVQAGH"
     gene            complement(4785..5678)
                     /locus_tag="VIG70_00635"
     CDS             complement(4785..5678)
                     /locus_tag="VIG70_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8249085.1"
                     /translation="MTRIFALLAALAVATTAAAQEGTLKKIKDSGAITIGHRDASVPF
                     SYYDDKQQPVGYAMDICAKVVDAVKSELKLPNLKTNYQLVTSANRIPLMANGTIDLEC
                     GSTTNNLARQEQVWFATTHFVTANRFVSKKSAKIGKLADLKGKTIVSTAGTTNIKQIT
                     EINGEQKLGMNIISANGHAEAFQMVETGRAVAFVMDDILLYSLVAQSRSPKDYDISKE
                     ALSVEPYGIMVRKDDAAFKKTVNGAINALYKSGEINKIYDKWFLKPIPPKNINLNVPM
                     SAQFKKVVANPTDSGDPAAYK"
     gene            complement(5967..6842)
                     /locus_tag="VIG70_00640"
     CDS             complement(5967..6842)
                     /locus_tag="VIG70_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8249086.1"
                     /translation="MDDLDFSKPAGTPKPAAPAPQPQAPLYIPAIARAFFESAGKEET
                     IAAGTVFFAENEKASRILLKRDKMYLLLDGEVSLSVKGKPLGTVKVGHIFGEMAAISD
                     SPRSATAVARSACRVIALDDKQFAAALEKTPQFALMMLGTLIQRLRGMIAQLSGVPNA
                     EAKDSRVFDKKLLAGLQAGLGDHAMVRYDKGKVIMVAGQTGALMYVVLDGRVSISIRG
                     ALVERVGPGGVFGEMALVDQSPRSANAVAESDCALLAINRNVFLNLVKSDPTFGISLL
                     GAMAERVRNTAAGVN"
     gene            6910..7152
                     /locus_tag="VIG70_00645"
     CDS             6910..7152
                     /locus_tag="VIG70_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249087.1"
                     /translation="MRAHKKDKPTGACNVCHALVNGLEHLNHRCNALVNGRRCYGIYK
                     SAITYLWDPCESCEATGKVGTQSCTACAGFGWIMYG"
     gene            complement(7139..>9302)
                     /locus_tag="VIG70_00650"
     CDS             complement(7139..>9302)
                     /locus_tag="VIG70_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEY8249088.1"
                     /translation="LLDITDNIVGERIVTPASTVRRDGDDPYLVVAADKGTATFSDIA
                     NGISIAHNFWLGDAFASGGSAGYDHKKMGITARGAWECVKRHFRELGVDTQKQDFTVA
                     GIGDMSGDVFGNGMLLSRHIRLVAAFDHRHVFLDPAPDPSKSFEERKRLFNLPRSSWA
                     DYDTKLISKGGGVFARSAKSVPLSPEVQKVLGVEAKTLTPTELIGAILKAPVDLLYNG
                     GIGTYVKSSRQPHAEVGDRANDAIRVNGADLRCKVVAEGGNLGFTQLGRVEFALAGGR
                     INTDAIDNSAGVSCSDHEVNIKILLGAAALPLEERNKLLVQMTDEVAALVLRDNTFQT
                     QSLSVSGVLAPSLLDPQERFIRYLEKTGRLNRGLEFLPGDEEFAERRAEKLGLTSPER
                     AVLLAYSKIALNDALVDSDVPDDPFISTALERYFPVPLRERFRGEIQAHPLRREIIAT
                     HVTNSMINRVGSTFVHRMQEETGAGAPDVVRAYLIVREAFGFVDLWRAVEALDGKVPD
                     RVQTAILIDGGRLMVRGTLWFLRHRSHLADLTRSIGHFQAGAERIASALAEILPASEK
                     AGFAAAAERLQKEGVPADLARRAAGSDTLFGVLDIVEVASSLKRDIDAVARAYFAIAG
                     ELEFPWLRAAIGMLAGDDHWHGLAKAALRDDLSSMLRALAHDVLSAEDGDLAAWKARN
                     AVPYERFRQVLADLRGAESPDLAMLSVAMRELRNLSRT"
BASE COUNT         1595 a         2980 c         3149 g         1578 t
ORIGIN      
        1 gagcaactcg tcgccgcgcc ggacaaccct gcgcgacagg ccgaggcgcg cgaggcgctc
       61 acgcggctat tgcaggcgat cgagtcgctg ccggcggcgc agcgcgaggc cttcctgctc
      121 cacgaggaag ccggcttgag cgtggcggaa atcgccgccg ccacgggcac caacgacgaa
      181 gcggcgaaga gccgcttgcg atatgcgatg gccaagctga aagcggcggt ggacgatggc
      241 tgacgaacgg aaaatctcgc agcgctatcg cgagctgccg cgcgaggagc cgccgcatca
      301 cctcgaccag gcgattctcg cggccgcgcg ccgcgccgcc gagattcggc cggcgccgct
      361 ggtcgtgccg agcgggcggc ggcgctggta tttcccgctc gccgccgccg cggtgatcgt
      421 cctcgccgtg gcggtcacga tgcacgtgga gcgcgagcaa cccgacgtcg aaatggcgga
      481 agcgccggtc gccgcatcac ctcccccggc gccgctcgcg cgcgaggagc aggccgcgcc
      541 ggcgcaggcc ccggccaagc gcgaggcgtt cacgcccgat ccgaagcccg cgccttctcg
      601 ggaccgagcc ccgggcgctg agctgccggc ggatcttcga aaggctcaga aagaggctca
      661 gaacgcgccg tcttcgccag cgcctccacc ggccgcgtcc gcgccggtac cggcgcagga
      721 cgctgtgagc ggcgcccgcg aatctgctcg cgcagacgcc ggcgcgaaac cgtcggcgcc
      781 gatgcgtcag gaatcccgtg tacgcgcctc aagcgcgcca ggtgcagcgc ccgccgtagc
      841 gggtgccctc ggagccttcg cctacacgtc gcccgagcaa tggctgcagg gcatcgacga
      901 tctgaagcgg caacgcaggc acgacgaggc cgaaaagcaa ctcgcggaat tccgcaagcg
      961 ctatcccgat taccggattc ccgaggctat cctggaaaag ttcgagaagc gctaatcact
     1021 ggatgacttc gtcggcacgc accagcactg actgagggac ccggattccg agcacctttg
     1081 cggtcttgag attgatgacc agctcgaact gggtcggctg ctcgatcggc agatctccgg
     1141 gtttggcccc cttgagaatc ttgtcgacgt acgtcgctgc ccggtagtgc aggctcgaga
     1201 agctagcgcc gtaggacagg agcatgccga gttgcgcagc acggcgttgc tgatgagccg
     1261 ccacgagtct gtgcttcgca gcgaacgcgg caaccgtgct cagattggca tcgagcagcg
     1321 cgtcgctgag gatgatcatg gcatccggtc gtttctgcgc cagcgagcta agcgcggagt
     1381 cgatctcctc tttgcgtcgc gcttcgaaag aatgcactgt tacgcggagg cgcttcgccg
     1441 ccgcctgcgt ctccttgaac tgaatgaccc gctcaggcac gtcgggattc cagagtagcg
     1501 ccacgtcgtt catcttcggc agtatctctc ggaggatctc gagccgcttt ccgccaagtt
     1561 gcggcgcgag cgtggtgagg ccggtgatcc tgccaccggg gcgcgccagg ctgtcggcga
     1621 gaccaccccc tacggggtcg cccgcccatg ccatcacgat cggcacgtcg cgcgtggcct
     1681 ttcgcgcgac tcgcgtgacg tgcgagccgc ccgtgaccag aacgtcgaca gggattgccg
     1741 ccaggtcggc cgcgagctgc ggcaggcgat cgatctttgc ttcggcgtac cgcacttcga
     1801 gccgcaggtt gcggccttcg acgtatccaa ggtcacgcaa gcctttgcgg aactgctcaa
     1861 gatgaggggc gtgccccgca gaagaaactg cgatgagtac gccgacagtc gcgatcttgc
     1921 tctgcgccaa ggccgccgcc gcgaacatcc acaggccggc aacgagcgcg cagcgcgtca
     1981 ccgcgtcaaa accatcattc ctcgagaagc gccagcgctc aggcgccgat gccgtgctcg
     2041 cgcgggactg gcgatcccgg tccgtcgggc cgggcgagga gcctcgcttc gggcacgccc
     2101 ttccggaatt gctcgaccag ctcgtccggg tcgaactcga ccccgatcgg gttcgcggca
     2161 aagctatccc cgcgcatgta tgcgttggcc gcttcggctg ttgcgaagca gtcgacctgg
     2221 aactcgagcc gattgccgtc gggatccttg tagtagagcg acagcgtcgg gccgtggcgg
     2281 atcggccagt acggcacgat gccttcggct ttcaggcgcg tgtacgtgcc gagcagatcc
     2341 gccaggctcc cgtaggtgta ggcgacgtgg tcgacacccg tatcgggcct ggtcgcacct
     2401 gcgccgcccg caggatcgag caccgacagg ttggcgaagg cgaaacggtg gtgctcgtcg
     2461 tcgtaggtta gaaaggcgag cacgggattg cggtactgca cctttgcctc gaagaccttc
     2521 tcgtaccagc cgatcatctc ctcgaagcgc cgcgtgcggt acacgatgtg cgcgaacttg
     2581 gaaggggcgg ccatggcgat ttcctcagtg atgcaggatc ttggagagaa acagctgcgc
     2641 gcgctcggag cgcggggtgc cgaagaactc gtccttcttc gcgtcctcga cgatgcggcc
     2701 ggcgtccatg aagatgacgc ggtgcgcgac cttgcgcgcg aagcccatct cgtgcgtgac
     2761 caccatcatg gtcatgcctt cgcgcgcgag ctcgaccatg acgtcgagca cctcgttgat
     2821 catctccggg tcgagcgccg aggtcggctc gtcgaacagc atcgcgatcg ggtccatcga
     2881 cagcgcgcgc gcgatggcga cgcgctgctg ctgaccgccc gagagctggc cggggtactt
     2941 gtccttgtgc gcgatcaggc cgacgcggtc gagcagcttc aggcccttgg cgcgcgcttc
     3001 gtcctcgctg cggccgagca ccttgatctg gcccagggtg aggttccggg tcacgctgag
     3061 gtgcgggaac agctcgaagt tctggaacac catgccgata cgcgagcgca gcttgggcag
     3121 gtccgtcttc gcgtcgtgga ccggcacgcc gtcgacggtg atctcgccct tctggaaggg
     3181 ctcgagcgca ttcaccactt taatgagggt cgacttgccg ctgccggaag gcccgcatac
     3241 caccacgacc tcgcccttgg cgacttcggt cgagcagtcc gcgagcacct ggaagctgcc
     3301 gtaccacttg ctgacctgcc tgatcgagat catcggatga tggcgatcct gtcctggagg
     3361 cgtttcacga ggagggacaa cgaataggag atgatgaagt acacgaccgc gacgaaggtg
     3421 tacatctcga ccaggcggta gtcgcggttg gcgaccttgg aggccgcgcc gaggaagtcg
     3481 gtcgccgaga tcacgtacac cagcgaagtg tcctggaaca ggatgatggt ctgcgtgagc
     3541 aggatcggca gcatgttgcg gaacgcctgg ggcagcacga tctggcccat cgactgccag
     3601 tagttgaggc cgagcgcgta gccggcccac acctggccgc gcgggatcga ctggatgccg
     3661 gcgcgcatga tctcgcagta atacgccgcc tcgaacatgg tgaacgtgac caccgccgaa
     3721 agccaggcgc cgacctggac cggccgatcg gccccggtga tccaggcgcc gatatagggc
     3781 acgaggaaga agaaccagaa gatcaccagc accagcggca ccgagcgcat caggttcacg
     3841 tagccgccgg caaccgccga cagcgccttg aacgccgaga gccgcatcat ggcgagcagc
     3901 gtgccgaaga tgatgccgcc ggtcatcgcg agaagcgtga gcgtgacggt gaaggtcatc
     3961 ccctccttga agagatagac ccaggagcgc tggatgacgt cgtaatcgaa gccggcgtcc
     4021 atctagtgcc ccgcctgtac cgtgtgggac ccgatcaggc cgggaacccg gaccttgcgc
     4081 tcgaggcccc gcatgaggag cacgacgacg aggttggtga ggaggtagat cacggtcgcc
     4141 gtggcgaagg cttcgaacac gcgaaagctg aactcctgca tggcgcgcgc acgcccggtg
     4201 agctcgagca ggccgatggt gagcgccacc gaggagttct tgatgatgtt catcgactcc
     4261 gaggtgagcg gcggcaggat gatgcggaac gcctgcggca ggaccacgta gcggtagctc
     4321 tggccgagcg tgaggccgag cgcggtgccg gccatgcttt ggccgcgcgg cagcgagttg
     4381 atccctgcgc gtacctgctc ggcgatgcgc accgaggtga agatgccgag gcacagcacc
     4441 gccgggatga acgagcccca cggcggcggc atctgcttga tccagtcgcc gagcgcctgc
     4501 ggcaggagct cgggcatgac gaagaaccac aggaacatct gcacgatgag cgggatgttg
     4561 cggaagatct cgacgtacag gttgcccgcg cgcaccaccc atggataagg cgtggtgcgc
     4621 agcgtgccga ccagcgcgcc gatcgccagc gcgatgatcc aagcggcgag ggccgtgacg
     4681 agcgtccagc ccaggccgac gatcaggtac tggaggtaag tgccggtccc gcccggctcg
     4741 gtctcgagca ggaccttcca gttccagttg tagcccacga aaggctactt atacgccgct
     4801 gggtccccgg aatcggtcgg gttcgcgacc accttcttga actgggcgct catcggcacg
     4861 ttcaggttga tgttctttgg cgggatcggc ttcaggaacc acttgtcgta gatcttgttg
     4921 atctcgcccg acttgtagag cgcgttgatc gcgccgttga ccgtcttctt gaacgccgca
     4981 tcgtccttgc gcaccatgat gccgtagggc tcgaccgaga gcgcttcctt ggagatgtcg
     5041 tagtccttag ggctgcgcga ctgtgcgacc aggctgtaga gcaggatgtc gtccatgacg
     5101 aacgccaccg cgcggccggt ctcgaccatc tggaacgcct cggcgtggcc gttggccgag
     5161 atgatgttca tgccgagctt ctgctcgccg ttgatctcgg tgatctgctt gatgttggtg
     5221 gtgccggcgg tcgagacgat ggtcttgccc ttcaggtcgg caagcttgcc gatcttggcc
     5281 gacttcttcg acacgaagcg gttggccgtc acgaagtgcg tggtggcgaa ccagacctgc
     5341 tcctggcgcg ccaggttgtt ggtggtggag ccgcactcga ggtcgatggt gccgttcgcc
     5401 atcagcggga tgcggttggc cgaggtgacg agctggtagt tggttttcag gttcggcagc
     5461 ttgagctcgc tcttcactgc gtcgaccact tttgcgcaga tgtccattgc gtagccgacc
     5521 ggctgctgct tgtcgtcgta gtaggagaag ggcaccgagg cgtcgcggtg gccgatggtg
     5581 atcgcgccgc tatccttgat cttcttgagg gttccctcct gcgcggcggc ggtcgtggcg
     5641 accgcgagcg cggcgagcag agcgaaaata cgggtcataa agactcctgg aaggttgttg
     5701 attgttgctc taacgcggaa attctacttt agttgacctg gggaaactct gaaaaagcgc
     5761 cgaatggtcg tccccgcgaa ggcggggacc cagttaagac gcgtgttctc acgctcaaag
     5821 ggagaagaga ctcaagaagt gaaatcgctt ggaagatcag gctgatcgag gagatgaatc
     5881 ccgagtggaa agacccttct gactaggcct tctaattttc tagactgggt ccccgccttc
     5941 gcggggacga cttcttcgga ccttccctag ttgacgccgg ccgccgtgtt gcgcacgcgc
     6001 tcggccatcg cgccgaggag cgagatgccg aaggtcgggt cggactttac gaggttcagg
     6061 aacacgttgc ggttgatggc gaggagcgcg cagtcgctct cggcgaccgc gttcgccgag
     6121 cgcggcgact ggtcgaccag cgccatctcg ccgaacacgc ccccgggccc gacgcgctcg
     6181 accagcgcgc cgcggatcga gatcgagacg cggccgtcga gcaccacgta catcagcgcg
     6241 ccggtctggc ctgcgaccat gatgaccttg cctttgtcgt agcgcaccat cgcgtggtcg
     6301 ccgagccccg cctggaggcc cgcgagcagc ttcttgtcga acacgcgcga gtccttcgcc
     6361 tcggcgttcg gcacccctga gagctgtgcg atcatgccgc gcagccgctg aatcagcgtg
     6421 cctagcatca tcagcgcgaa ctgcggcgtt ttctccaggg cggctgcgaa ctgcttgtcg
     6481 tcgagcgcga tcacccggca cgcgctgcgc gccaccgcgg tggcgctgcg cggcgaatcg
     6541 ctgatcgccg ccatctcgcc gaagatgtgc ccgaccttca ccgtgccgag cggcttgccc
     6601 ttgacgctga gcgacacctc gccgtcgagc agcaggtaca tcttgtcgcg cttgaggagg
     6661 atacggctcg ccttctcgtt ctcggcgaag aacacggtcc cggcggcgat ggtctcttcc
     6721 ttgccggccg actcgaaaaa cgcgcgcgcg atcgccggga tgtagagcgg ggcctggggc
     6781 tgcggtgccg gagccgcggg cttcggagtg cccgcgggct tgctgaaatc cagatcgtcc
     6841 atcagttcct caaaaacgca tcatagtgcc tttgccggcg ggtttgcgcc ttgggacgat
     6901 aggcaggcca tgcgtgcgca caagaaggac aagccgaccg gagcctgcaa cgtctgccac
     6961 gccctcgtca acgggcttga gcatctcaat caccgctgca acgcgctcgt caacggccgg
     7021 cgctgttatg gcatctacaa gagcgccatc acgtacctgt gggacccatg cgagtcctgc
     7081 gaggccaccg gcaaggtcgg aacccagtcg tgcaccgcat gcgcaggctt cggctggatc
     7141 atgtacggct gagattgcgc agctcgcgca tcgcgaccga gagcatcgcg aggtccggcg
     7201 actcggcgcc acgcagatcg gcgagcacct ggcggaagcg ctcgtagggc acggcgttgc
     7261 gcgccttcca ggccgcgaga tctccgtcct cggcgctcag cacgtcgtgc gcaagggccc
     7321 gcagcatgct tgagaggtcg tcgcgcaggg ccgccttggc gaggccgtgc cagtggtcgt
     7381 cgcccgcaag catgccgatg gcggcacgca gccaggggaa ctcgagctcg cccgcgatcg
     7441 cgaaataagc gcgcgccacc gcgtcgatgt cgcgcttcag gctcgaggcg acttcgacga
     7501 tgtcaagcac gccgaacagg gtgtcggacc cggcggcgcg gcgcgcgagg tccgcgggca
     7561 cgccttcctt ctgcaggcgc tcggccgcgg cggcgaagcc cgctttctcc gaagccggga
     7621 ggatctccgc gagggccgac gcgatccgtt cggcgccggc ctggaaatgc ccgatcgagc
     7681 gcgtgaggtc ggcgaggtgc gagcggtggc gcaggaacca gagcgtgccg cgcaccatca
     7741 ggcgtccgcc gtcgatgagg atcgcggtct gcacgcggtc gggcactttg ccgtccagcg
     7801 cctcgaccgc gcgccagagg tccacgaagc cgaacgcttc gcgcacgatg aggtaggcgc
     7861 gcaccacgtc gggcgccccg gcgccggtct cttcctgcat gcggtgcacg aaggtcgagc
     7921 cgacgcggtt gatcatgctg ttggtgacat gggtggcgat gatctcgcgg cgcagcgggt
     7981 gggcctggat ctcgccccgg aaccgctcgc gcagcggcac cgggaagtag cgctcgagcg
     8041 cagtggagat gaaggggtcg tcgggcacgt cggagtcgac cagcgcgtcg ttgagcgcga
     8101 tcttcgagta cgcgagcagc accgcgcgct cgggcgaggt gagcccgagc ttctccgcgc
     8161 gccgctcggc aaactcttcg tcgcccggca ggaattcgag cccccggttg aggcgcccgg
     8221 tcttttccag gtaccggatg aagcgctcct gggggtcgag caggctcggc gccaggacgc
     8281 ccgacaccga caggctctgg gtctggaagg tgttgtcgcg cagcaccagc gcggcgacct
     8341 cgtcggtcat ctggaccaga agcttgttgc gctcctcgag ggggagggcg gccgcgccga
     8401 gcaggatctt gatgttgacc tcgtggtcgg agcacgagac gccggccgag ttgtcgatgg
     8461 cgtcggtgtt gatgcgcccg cccgccagcg cgaactccac tcgccctagc tgcgtaaagc
     8521 cgaggttgcc gccttcggcc accaccttgc agcgcaggtc ggcgccgttc acgcggatgg
     8581 cgtcgttcgc gcggtcgccg acctcggcgt gcggctggcg gctcgacttc acgtaggtgc
     8641 cgatgccgcc gttgtagagg agatccaccg gcgccttgag gatcgcgccg atgagctcgg
     8701 tcggcgtcag ggtctttgct tcaacgccca gcactttctg aacttcgggc gagagcggca
     8761 ccgacttcgc gctgcgcgca aatactccgc cgcctttgga aatcagcttc gtgtcgtaat
     8821 ccgcccagga ggaccgggga agattgaaga gacgctttcg ctcctcgaag ctcttgctgg
     8881 gatcaggtgc tggatcgaga aagacatggc ggtggtcgaa cgccgccacc agtcggatat
     8941 ggcgcgaaag cagcatgccg ttgccgaaca cgtcgcccga catgtcgccg ataccggcga
     9001 cggtgaaatc ctgcttctgc gtgtcgaccc cgagctcgcg gaaatggcgc ttcacgcact
     9061 cccaggcgcc gcgcgcggtg atgcccatct tcttgtggtc gtagccggcc gaaccgcccg
     9121 acgcgaacgc atcgccgagc cagaaattgt gcgcgatgga aatcccgttg gcgatatcgg
     9181 agaacgtcgc agtgcccttg tcggccgcga ccacgagata cggatcgtcg ccgtcgcgac
     9241 gcacggtaga agcaggcgtc acgatgcgct cgccgacaat gttgtcggta atgtccagca
     9301 gg
//
LOCUS       DATBSZ010000010         9897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1094497, whole genome shotgun sequence.
ACCESSION   DATBSZ010000010 DATBSZ010000000
VERSION     DATBSZ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094497"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2284)
                     /locus_tag="VIG70_00655"
     CDS             complement(<1..2284)
                     /locus_tag="VIG70_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY8249089.1"
                     /translation="MKLKSMASGMIAVGLFTLSPSVLTHDDDDDGGRHHGKPPPAAKP
                     ANCCTPGGKDYPTHSGNLGNQAYSGLTQINKGNIKRLGPVWRTHVSAEAPATTDTGQQ
                     TTPIVVDGVIYLDTPNGSVIAVDGATGMTKWKWTPTAFETNGTRRGVSVGDGKVYTLA
                     DGNRVVALNKDTGLEAWVVQPTGPNGASLGNIDKVATVYHDGMVYVGTNDGNRGAAFA
                     VRSSDGSLVWSFYGTGDHGELTDVNGKKTDVAGTWQTGNGCALMAGATPWIHGALDPE
                     LGMAYYAFGNARSCGSSQDGGTRPGENLFANSVVAVDLKTGAYKWHHQSIRHDVWDMD
                     NVHPLLLADVRINGRTRKAIYHGSKSGHLFVLDRANGKALLRVDEVPMTVDSRQVNWP
                     AQPFPNRFLPHCLNWQALDPKNIPGDPWRAVPNYNGYQPNAAGQLVYTEPNYLDPDKP
                     FVTYPPEYGATHRKGCMYDTHWDLPVLSTTSQNGGPDWSAYSFSHKRGLIFIPYGVNP
                     VAHWRGAGGNGQRAIGQYQTGGILALDASTNKVVWRNHLGLDMGHNQSPLSTASDLLF
                     VGMFDGNFLALDAATGKELWRFQTGAAISAGAITYAIDGEQYVAIFSAGTGIPYGNSI
                     TEGDSLWAFKLGGRYKTASGSSEDPTPAPLVIRRPVGGNAVEGSTVSNTIYLARANRT
                     TDTAAARDGVAAGAMNPTHMRVPVGTTVTFLNPGAATFPLFPNLKPHCATQYFEGLFN
                     PKLNPGESFQYTFTRAGEYFF"
     gene            complement(2414..3277)
                     /locus_tag="VIG70_00660"
     CDS             complement(2414..3277)
                     /locus_tag="VIG70_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249090.1"
                     /translation="MSKLKFSFALAAAAGLIAWWWAEVPATDGAPPAAQSPRTLAASE
                     PSPVDLKFQRRALSTASRDPFGTPPKPPPPARPAPRAEAPPPPKAPPLPFQYDGSGAL
                     QGKSFVFLKRQNRTFMVSAGDTIEGTYGVERVARDRVVLRYLPLGIQQVMMYATGAEG
                     PPEVADAPGIGRSVALQVDMPAEVVLGDEFVVTVGLPAGALKATVEVVYDAEVLRMLG
                     AKQRRPGRAIVEVAGGSSATRAQVRFKVLADVPVSTEIDLQVNATDANGKRVAVWAPS
                     AHTVSLVLPGA"
     gene            3501..4325
                     /locus_tag="VIG70_00665"
     CDS             3501..4325
                     /locus_tag="VIG70_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY8249091.1"
                     /translation="MKHDYSLAHLTALHLSPPDLVAAAAAGGYRYVGLRLTRVADDDP
                     FYPLATDAALMKATKARLADTGVEVLDIELARLDPETSPESLQGILEAGAELGARHVI
                     GQLPDPVRSRKVARFAALCDLAKPLGLTVELEFPSWTETPSLAEAAYVLKAVNRPNAG
                     MLVDLLHFARSRSSVEELRMLPRSWFRFAHVCDAPAEIPSSTESLIHTARAERLFPGE
                     GGIDVRGILAALPPDIPYALEIPRVTLSALVGEKENARLAIMAARRHLEALAEPAQ"
     gene            complement(4330..5211)
                     /locus_tag="VIG70_00670"
     CDS             complement(4330..5211)
                     /locus_tag="VIG70_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY8249092.1"
                     /translation="MNLDHFAMDTATLAGPLEARLSAVRAAGFSHVVLNASEIAGYPG
                     GEQAAAAAVRASGLHVCGFQVLRDFEGLSGHLHDYKVDVAKAMLRMCRTVGSELLVAW
                     SSTSNHASGDAAALVRDLRKLATLALPLGIRIGYEALSWGRHVDAYPKAAQLVDEADR
                     ANLGLVLDSFHVLAARTALDELDYVDSRKIFLVQLSDFLWHEAPSAEERGEAGRHARV
                     FPGEGVHSAELADFIRRLDEKGYRGDYSFEVFNDDYLQLPPATVAGRALKSAKWAIDH
                     VSRRSLPLRRPGARRAT"
     gene            complement(5208..6083)
                     /locus_tag="VIG70_00675"
     CDS             complement(5208..6083)
                     /locus_tag="VIG70_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY8249093.1"
                     /translation="MTIQESIPEPTNPIAMDGIEFIEYATSQPQAFGALLQKMGFSAV
                     ARHRSREVMLYRQGAMNVIVNSHPDALPGLAAPVETPMLAAMAFRVRDASQAYRHSLD
                     CGAWEMPTRASAMELNIPGIHGVGDSLIYFVDRYRDFSIYDVDFVPLAGAAPRPPALA
                     GLHWFGVVQAILNDRTNDWVDFYRTLFGFTILPEGKYFGVLPKGTLLESPCHTFYLQL
                     IEPPPGAEDIHWDEGLVRVGLGAPDVRAAVRALKERGIVFIDHGAVQPTEKGALTQVY
                     QGAVTFELVVSHLKP"
     gene            complement(6093..7202)
                     /locus_tag="VIG70_00680"
     CDS             complement(6093..7202)
                     /locus_tag="VIG70_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY8249094.1"
                     /translation="MPSALEVYDELTSRRTRFVRIDELCRRASGLPPQEQLQAEAQLP
                     LKQKKGLERAQADFLSAVLADPARGAHLCHAMLLPREDSLARLTDYQNRGELDLGGAH
                     IQRHGKASVVTMRNPRYLNAEDETTLAPLEIAIDLALLDPATQICILRGGSVEHPKYA
                     GRRLFGAGINLTHLYHGKIRYLWYLERDLGLVNKLYRGLALRERSPEEGSVEKVWVAA
                     VEGFAIGGHCQILLAVDHTLAAADAYMTLPARKEGIVPGAANLRLPRFVGDRIARQAI
                     MAERRLDCASPEGRLICDEIVEPGGMDEGLAATVERLTGSGVVSAASNRRAFRIAQEP
                     LDQFRRYMAIYAREQAECHFSAALVANLEKNWKAK"
     gene            complement(7242..8039)
                     /locus_tag="VIG70_00685"
     CDS             complement(7242..8039)
                     /locus_tag="VIG70_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY8249095.1"
                     /translation="MRLSNMDTTLKPHGDPNFMMSLARGLDVIRGFSREKRYMSIAQL
                     SYKTGIPRAAVRRCLYTLRELGYVECADGRNFSLRPKLLGLGHAYLSSTPLVVSAQPF
                     LDRVSEAANESCSLATLDGEDILYVARSLTSRIISVTLNVGSRLPAYCTSIGHVLLAS
                     LLPAELDGYLSKTSLRPYTERTLTSASRLRELLEQVRAQDYAIADQMMEIGVRSIAVP
                     VRDAAGRVVAGINVIVQAERVSLRDMKALFLPHLQIAARDLGAQLIP"
     gene            8137..8589
                     /gene="aroQ"
                     /locus_tag="VIG70_00690"
     CDS             8137..8589
                     /gene="aroQ"
                     /locus_tag="VIG70_00690"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY8249096.1"
                     /translation="MRILMLHGINLNMFGKRDPAQYGTITLAEIDERLKKLGRELGVE
                     LECFQTNHEGAMCERIHKAFAENVDAVLINAGAWTHYSYGIRDALAILKVPVVEVHMS
                     NIHAREEFRRHSVLAAVAKGQIAGFGVDSYLLALRAAVSAVQASNKTT"
     gene            8586..9428
                     /locus_tag="VIG70_00695"
     CDS             8586..9428
                     /locus_tag="VIG70_00695"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY8249097.1"
                     /translation="MKLLVGLIGAGIQRSLSPALQEEEARHHGLRLHYQLIDLDAAGV
                     GPEVLSELIRAARIMRFAGLNITFPCKQSVIPLLDSLSEEARTIGAVNTVVREGDRLV
                     GHNTDGSGWSWGFRRALPGADLSRVVLLGAGGAGSACADAVLRLGAQQLVVVDREHSR
                     ATELAARLNGNFAGRRAAAAADAAAALRGATGLIHATPVGMTAHPGMPLPEELLPRSL
                     WVSEVVYVPLETPLLKAARRIGCQTADGGHMNVGQAMGGFKLFTGRDADAVRMDAHFR
                     KLVA"
     gene            9425..>9897
                     /locus_tag="VIG70_00700"
     CDS             9425..>9897
                     /locus_tag="VIG70_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY8249098.1"
                     /translation="MTQPIGLAALTVLELPAAEQVSVAAQAGYTHVGLRLIPVAGQTL
                     PPFDVDEIERRLADTGIRVLDLEVFRLSADTRVADFEPALATAARLGASELLVHGADP
                     DEARLVENFGRLCDLAARYRLAANVEPMPWVEVSSVSKTKRVLDQAARKNSALLV"
BASE COUNT         1529 a         3299 c         3392 g         1677 t
ORIGIN      
        1 tgaagaagta ctctcctgca cgggtgaacg tgtactggaa gctctcgccc ggattcaact
       61 tcgggttgaa caatccctcg aagtactggg tcgcgcagtg cggcttcagg ttggggaaga
      121 gcgggaaggt cgctgccccc gggttcagga acgtgacggt tgtgcccacc ggaacgcgca
      181 tgtgcgtcgg attcatcgcg ccagcggcta cgccatcccg cgcggcagcg gtgtccgtcg
      241 tcctgttcgc tcgcgcgagg tagatcgtgt tgctcactgt gctgccttcc actgcgtttc
      301 caccgaccgg gcggcgaata accagcggcg ccggcgtcgg atcctcgctg ctgcccgagg
      361 ccgtcttgta tctgccgccc agcttgaacg cccacagcga atcaccttcc gtgatcgagt
      421 tgccgtaagg aatgccggtg ccggcggaaa agatggcgac gtattgctcg ccatcgatcg
      481 cgtacgtgat cgcacccgca ctgatggccg cgccggtctg gaagcgccac aactccttgc
      541 ccgttgctgc gtccagcgca aggaaattgc cgtcgaacat gccgacgaaa agcaggtcgc
      601 tcgcggtcga gagcgggctc tggttgtgcc ccatgtccag gcctaggtgg ttcctccaga
      661 ccactttgtt ggtcgaggcg tcgagcgcga ggatgccgcc cgtctggtat tgcccgattg
      721 cgcgctggcc gttgccgccc gcgccgcgcc aatgtgcgac cgggttgacg ccgtacggaa
      781 tgaagatcag cccgcgcttg tggctgaaag aataggccga ccagtcgggt ccgccgttct
      841 ggctggtggt cgagaggacc ggcagatccc agtgcgtgtc gtacatgcag cccttgcggt
      901 gcgtcgcgcc gtattcgggc ggataggtca cgaagggctt gtccgggtcg aggtagttgg
      961 gctcggtgta aacgagctgc cccgccgcat tgggctgata gccgttgtag ttcggcaccg
     1021 cgcgccacgg atcgcccggg atgttcttcg gatcgagcgc ctgccagttg aggcagtgcg
     1081 gcaggaaacg gttcgggaac ggttgcgccg gccagttcac ctgccgcgaa tccaccgtca
     1141 tcggcacctc gtcgacccgg agcagggcct tgccgttggc ccggtcgagc acgaacaggt
     1201 gcccggactt gctgccgtgg tagatcgcct tgcgcgtccg gccgttgatc cgcacgtcgg
     1261 ccagcaggag cggatggacg ttgtccatgt cccacacgtc gtggcgaatc gactggtggt
     1321 gccatttgta cgcgccggtc ttcaggtcca ccgcgacgac gctgttggcg aacagattct
     1381 caccgggacg cgttcctccg tcctgggacg agccgcagct gcgagcgttg ccgaacgcgt
     1441 agtaagccat gccgagctca ggatcgagcg ctccgtggat ccagggcgtg gcgccggcca
     1501 tcaacgcaca gccgttgccc gtttgccagg tgccggcgac atcggtcttc ttgccgttca
     1561 catcggtcaa ctctccatgg tccccggttc catagaacga ccagaccaag ctgccgtcac
     1621 tggatcgcac ggcaaacgcc gcaccgcggt tgccatcatt ggtgcccaca taaaccatgc
     1681 cgtcgtgata aacggtggcg accttgtcga tgttcccgag agaggcaccg ttggggcccg
     1741 tgggctgcac gacccaggcc tccagtccgg tgtccttgtt cagggcgacg acgcgattgc
     1801 cgtcggcaag cgtgtacacc ttcccgtcac cgaccgagac gccgcggcgc gtgccgttgg
     1861 tttcgaacgc cgtcggcgtc cacttccact tggtcatacc cgtggcgccg tcgaccgcga
     1921 tgacgctccc gttcggcgtg tcgaggtaga tgacgccatc gaccacgatc ggcgtggtct
     1981 gctggccggt gtccgtggta gcgggcgcct cggccgaaac gtgcgttcgc cataccggcc
     2041 cgagccgctt gatgttcccc ttattgatct gcgtcagccc ggaataggcc tggttgccca
     2101 ggttgccgct atgggttggg tagtccttgc cgccgggcgt gcagcagttc gcgggcttgg
     2161 ccgcgggcgg gggcttgcca tggtggcggc cgccgtcgtc gtcatcgtcg tgcgtgagca
     2221 cgctcggcga cagcgtgaac aggccgacag caatcatccc cgacgccatg gacttgagtt
     2281 tcattgcgct ggtctcccgc gttgttggca gagttctatc aggtagaacc ctattccaaa
     2341 actgtaggag gcccggcctg cgcggtcaac ggcgcggggc cttaccaaag cagatggcgg
     2401 gcgctacttt ggcttacgcg ccgggcagga ccaggctcac cgtgtgcgcg gaaggcgccc
     2461 acaccgcgac tcgcttgccg ttggcgtcag tcgcgttcac ctgcagatcg atctcggtgg
     2521 agaccggaac gtccgccagc accttgaagc gcacctgcgc gcgcgtggcg ctgctgccgc
     2581 ccgccacctc cacgatcgcc cgtcccggcc ggcgctgctt cgcgcccagc atcctcagca
     2641 cctccgcgtc gtacacgacc tcgacggtgg ccttgagcgc gccggcgggc aggccgaccg
     2701 tgaccacgaa ctcatctccc aacacgactt cggcgggcat gtcgacctgc aaagcgacgc
     2761 tgcgcccgat ccccggcgca tccgcgactt cgggcggccc ctcggcccca gtcgcgtaca
     2821 tcatcacctg ctggatcccg agcggaaggt aacgcagcac gacgcgatcc cgcgccaccc
     2881 gttccacgcc atacgttcct tcgatcgtat cgcctgcgct caccatgaaa gtgcggttct
     2941 ggcgcttcag gaagacgaag ctttttccct gcaacgcgcc gcttccgtcg tactggaagg
     3001 gcagcggcgg cgcttttggc ggcggtggag cttcggcgcg aggggctggt cgcgccggcg
     3061 gcggcggttt cggcggcgtg ccgaaaggat cgcgcgatgc cgtggaaagg gcgcgccgct
     3121 ggaacttcag gtcgaccggg gagggctcgg aggcggcgag cgtacggggc gattgcgcgg
     3181 ctggaggggc gccgtcggtg gccgggactt ctgcccacca ccacgcgatg agccccgcgg
     3241 cggccgcgag cgcaaacgag aatttcagct tgctcatgct cgggcgatca aggattgcag
     3301 acacgcagca aagcagccac ccccatcgcg ctttgttcgc gcgtcaggca ccactgcgac
     3361 ttttccttgg caacactact atgttcattc actcctcggc cggccaatcg ggcgattaac
     3421 gcccattatt ctaggcgtta gcttgacgca cgccggagcg gggttcgaaa tatccgcatg
     3481 ccgcaaggag cgcacgcccg gtgaagcacg actactctct cgctcacctg accgcgctgc
     3541 acctttcgcc gcccgatctg gtcgcggcgg cggcggcggg cggctaccgc tacgtcggcc
     3601 tgcgcctgac gcgcgtcgcc gacgatgacc ctttctatcc gctcgcgacg gatgccgctc
     3661 tgatgaaggc gaccaaggcg cgcctcgccg acaccggcgt cgaggtgctg gacatcgagc
     3721 tcgcgcgcct ggacccggag acgtcgccgg agtcgctgca gggaatcctg gaagccggcg
     3781 ccgagctggg tgcgcgccac gtcatcggcc agctgcccga tccggtccgc agccgcaagg
     3841 tggcgcgctt tgccgcactg tgcgacctcg ccaagcctct ggggctgacc gtggagctcg
     3901 agttcccgtc gtggaccgaa acgcccagcc tcgccgaagc cgcctacgtg ctcaaggcgg
     3961 tcaaccgtcc caacgcgggc atgctggtcg atctgctgca tttcgcccgc tcgcgctcga
     4021 gcgtcgagga gctgaggatg ctgccgcgct cgtggttccg cttcgcgcac gtctgcgacg
     4081 cgccggccga gatcccctcg agcacggaga gcctcatcca tacggcgcgc gccgagcgcc
     4141 tgttccccgg cgagggcgga atcgacgtgc gcgggatcct ggccgcgctg ccgcccgaca
     4201 tcccctatgc cttggagatt ccgcgcgtca cgctgtcggc gctggtgggc gagaaggaga
     4261 acgcccggct cgcgatcatg gcggcgcgcc ggcacttgga agcgctcgcc gagccggcgc
     4321 aatgattctt caggtggcgc ggcgcgcgcc gggacggcgc agcggcaagc tgcggcgcga
     4381 gacgtggtcg atcgcccact tggccgactt cagcgcgcgg ccggcaaccg tggccggggg
     4441 cagctgcagg taatcgtcgt tgaacacctc gaagctgtag tctccgcgat agcccttctc
     4501 gtcgagccgg cgaatgaagt ccgccagctc ggcgctgtgc acgccttcgc cggggaaaac
     4561 gcgcgcatgc cggccggctt cgccgcgctc ctcggcggag ggcgcttcgt gccacaggaa
     4621 gtcggacagt tgaaccagga agatcttcct ggagtcgacg tagtcgagct cgtcgagcgc
     4681 cgtgcgggca gccagcacgt ggaacgagtc gagcaccagt ccgaggtttg cgcggtcggc
     4741 ctcgtcgacg agctgcgccg ctttcgggta ggcgtcgacg tgccgtcccc acgatagcgc
     4801 ttcatagccg atgcggatgc cgagcggcag cgcgagcgtc gcgagcttgc gcagatcgcg
     4861 caccagcgcg gctgcgtcgc cgctcgcgtg gttcgaggtg gaagaccagg ccaccagcag
     4921 ctccgagccc acggtgcggc acatgcggag catggccttg gccacgtcca ccttgtagtc
     4981 gtgcaggtgg cccgacaggc cctcgaaatc gcgcaggacc tggaagccgc agacgtgcag
     5041 gccgctcgcg cgcaccgccg cggcggccgc ctgctctccg ccggggtagc ccgcgatctc
     5101 cgaggcgttg agcacgacgt ggctgaagcc cgcggcgcgt accgcgctca gcctcgcctc
     5161 gagcggcccg gcaagggtcg cggtatccat ggcgaagtga tcgaggttca cggcttcaga
     5221 tggctcacca ccagctcgaa ggtgaccgct ccctggtaga cctgcgtcag ggcccctttt
     5281 tcggtgggct gcaccgcgcc gtggtcgatg aacacgatgc cgcgctcctt gagcgcgcgc
     5341 accgcggcgc gaacatccgg cgcgcccagc cctacgcgga ccaggccttc gtcccagtga
     5401 atgtcctccg cgcccggcgg cggctcgatc agctgaaggt agaacgtgtg gcaggggctc
     5461 tcgagcagcg tgcccttggg caggacgccg aagtacttgc cttccggcag gatcgtgaag
     5521 ccgaagagcg tgcggtagaa gtcgacccag tcgttggtgc gatcgttgag gatggcctgg
     5581 accacgccga accagtgcag cccggcgagc gcgggcgggc gcggcgcggc gccggcgagc
     5641 gggacgaagt cgacgtcgta gatggaaaaa tcgcggtagc ggtcgacgaa gtagatgagg
     5701 ctgtcgccca cgccgtggat gcccgggata ttgagctcca tggcagaggc gcgcgtcggc
     5761 atctcccagg cgccgcagtc gagcgagtgc cggtaggctt gcgacgcgtc gcgcacgcgg
     5821 aaggccatgg ccgcgagcat cggcgtttcc accggcgcgg cgagtccggg gagcgcatcg
     5881 gggtgcgagt tcacgatgac gttcatcgcg ccctgccggt agagcatcac ctcgcgcgag
     5941 cggtgccgcg ccactgccga gaatcccatt ttctgcagca gggcgccgaa cgcctgcggc
     6001 tgggaggtgg cgtactcgat gaactcgatg ccgtccatcg cgatcgggtt cgtcggttcc
     6061 gggatgctct cctggatggt catctgcccg ccttacttgg ctttccagtt tttctcgagg
     6121 tttgcgacca gcgcggcgct gaagtggcac tcggcctgct cgcgtgcata gatcgccatg
     6181 tagcgcctga actgatcgag cggctcctgc gcaatgcgaa acgcgcgccg gttgcttgcc
     6241 gcgctgacca cgccggagcc agtcaggcgc tcgaccgtcg ccgcgagccc ttcgtccatc
     6301 ccgcccggct cgacgatctc gtcgcagatc agccgccctt ccgggctcgc gcagtccagg
     6361 cgacgctcgg ccatgatggc ctggcgcgcg atccgatcgc cgacaaagcg cggcagacgc
     6421 aggttggcgg cgcccggcac gatgccttct tttctcgccg gcagcgtcat gtacgcatcg
     6481 gccgccgcga gcgtgtggtc gacggcgagc aggatctggc agtggccgcc gatggcgaag
     6541 ccctccaccg ccgcgaccca gaccttttca accgaaccct cctcgggcga tcgctcgcgc
     6601 agcgcgagac cccggtagag cttgttgacc aggccgaggt cgcgttccag gtaccacagg
     6661 tagcggatct tgccgtgata taggtgagtc aggttgatgc ccgcgccgaa gaggcgccgc
     6721 cctgcatact tcgggtgctc gacgctcccg cctctcagga tgcagatctg ggtcgcgggg
     6781 tcgaggagcg ccaggtcgat cgcgatctcg agcggcgcga gcgtagtctc gtcctcggcg
     6841 ttcaggtagc gcgggttgcg catggtcacc accgacgcct tgccgtgccg ctggatatgc
     6901 gcgccgccca ggtcgagctc gccccgattc tggtagtcgg tgagcctggc gagcgaatcc
     6961 tcgcgcggca ggagcatcgc gtggcacagg tgcgcgccgc gcgccggatc cgcgagcacc
     7021 gccgagagaa agtcggcctg cgcgcgctcc agcccctttt tctgcttcag cggcagctgc
     7081 gcctcggctt gaagctgctc ctgcggcggc aagcccgatg cgcgccggca aagctcgtcg
     7141 atgcgcacga accgggtacg gcggctggtc agctcgtcgt agacctcgag cgcgctgggc
     7201 atgcccgata gtatgcgctg gcgcgcgagg ccgcgcggtg ctcaagggat gagctgggcc
     7261 ccgagatcgc gcgccgcgat ctgcagatgc ggcaggaaga gcgccttcat gtcgcgcagc
     7321 gaaacgcgtt ccgcctggac gatgacgttt attccggcga ccacccggcc ggccgcgtcg
     7381 cgcaccggca ccgcgatgga gcgcacgccg atctccatca tctggtcggc gatggcgtag
     7441 tcctgggcgc gcacctgctc gagcagctcg cgcagccgcg aggccgaagt gagggtgcgc
     7501 tcggtgtacg gtctcaggga ggtcttgctg aggtatccgt cgagctccgc cgggagcagg
     7561 ctggcgagca ggacgtggcc gatggacgtg caataggctg gcagccggct gccgacgttc
     7621 agcgtcaccg agatgatgcg cgaggtcagc gagcgcgcga cgtagaggat gtcctcgccg
     7681 tccaaagtgg cgagcgagca ggactcgttc gctgcctcgc tcacccggtc gaggaatggc
     7741 tgggcgctga ccaccagcgg ggtcgatgaa agataggcat gacccaggcc gagcagcttc
     7801 ggccgcagcg aaaagttgcg cccgtcggcg cattccacgt agccgagctc gcgcagcgta
     7861 tagaggcagc ggcgcaccgc ggcgcgcgga attccggtct tgtagctgag ctgcgcgatc
     7921 gacatgtagc gcttctcgcg cgagaagccg cggatcacgt cgaggccgcg cgcgagcgac
     7981 atcatgaagt tcgggtcgcc gtgcggtttg agcgtggtgt ccatgttcga taaacgcaca
     8041 aaaggtcgat aatcgcgtaa gcccatggta cgccccggcc tgcggcattt gcaacgggac
     8101 ctataatcga tcgcggccac caatcccggg agacccatgc gcatcctgat gctccacggc
     8161 atcaacctga acatgttcgg caagcgcgat ccggcgcagt acgggaccat caccctggcc
     8221 gagatcgacg agcgcctgaa gaagctgggc cgcgagctcg gcgtggagct ggaatgcttc
     8281 cagaccaatc atgagggcgc gatgtgcgag cgcatccaca aagcctttgc cgagaacgtg
     8341 gacgccgttc tgatcaacgc aggcgcctgg acgcactaca gctacggcat ccgcgacgcg
     8401 ctggcgatcc tcaaggtgcc ggtcgtagag gtccacatgt cgaacatcca cgcgcgcgag
     8461 gagttccgcc gccactcggt tctggcggca gtcgcgaagg gacagatcgc cggcttcggc
     8521 gtcgacagct acctgctcgc tctccgagct gcggtgtctg cggtgcaagc ctccaacaag
     8581 acgacgtgaa gttgctggtc gggctgatcg gggccgggat ccagcggtct ctttcgcccg
     8641 cgctccagga agaagaagcg cgtcaccacg ggctgcgcct gcattaccag ctgatcgacc
     8701 tggacgcggc cggcgtcggc ccggaggtgc tgtccgagct gatccgcgcc gcgcgcatca
     8761 tgcgcttcgc cggcttgaac atcacctttc cctgcaagca gtcggtgatc ccgctgctcg
     8821 attcgctttc ggaggaagcg cggacgatcg gcgcagtcaa tacggtggtg cgcgaaggcg
     8881 accgcctcgt cggccacaac accgacggct cgggctggag ctggggcttc cggcgcgcgc
     8941 ttccgggggc cgatctgtcg cgcgtggtgc tgctcggcgc cggcggcgcg ggctctgcgt
     9001 gcgccgacgc ggtgttgcgc ctgggcgcgc agcagctcgt cgtcgtcgat cgcgagcatt
     9061 cgcgcgcaac cgagcttgcg gcgcggctga acggcaactt cgccggccgc cgcgcagccg
     9121 ctgccgccga cgccgcggcg gcgctgcgag gcgcgacggg gttgatccac gctacgccgg
     9181 tcggcatgac cgctcacccc gggatgccgt tgccggagga gctcttgcct cgctcgctgt
     9241 gggtgtcgga ggtggtctat gtgccgctgg aaaccccgct gttgaaggct gcgcggcgca
     9301 tcggctgcca gaccgccgac ggcggccaca tgaatgtcgg tcaggcgatg ggcggcttca
     9361 agctgtttac cggccgcgat gccgacgcgg ttcggatgga cgcgcacttc cgcaagcttg
     9421 tcgcgtgacg cagccaatag gccttgcggc gctcacggtg ctggagctgc cggcggccga
     9481 gcaggtgtcc gttgcggcgc aggccgggta cacgcatgtc ggcctgcgtc tcatcccggt
     9541 ggccgggcag acgctgccgc cgttcgacgt cgacgagatc gagcgtcggc tcgccgacac
     9601 cggcattcgc gtgctcgacc tcgaagtgtt ccggctttcg gcggacacca gggtcgcgga
     9661 tttcgagccg gcgctcgcca ccgccgcgcg gctgggcgcg agcgagctgc tggtgcacgg
     9721 cgcggacccg gacgaggcgc gcctggtcga gaacttcggc cgcctgtgcg acctcgccgc
     9781 gcgctatcgc ctggccgcca acgtcgagcc gatgccctgg gtcgaggttt ccagcgtctc
     9841 gaagacgaag cgggtcctcg atcaggcggc acggaagaat tccgcgctgc tggtgga
//
LOCUS       DATBSZ010000011        18030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_889516, whole genome shotgun sequence.
ACCESSION   DATBSZ010000011 DATBSZ010000000
VERSION     DATBSZ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18030
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889516"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /gene="ssrA"
                     /locus_tag="VIG70_00705"
     tmRNA           complement(<1..126)
                     /gene="ssrA"
                     /locus_tag="VIG70_00705"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(179..1054)
                     /locus_tag="VIG70_00710"
     CDS             complement(179..1054)
                     /locus_tag="VIG70_00710"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY8249099.1"
                     /translation="MGAGAVGCYYGAVLARAGHEVTLIGRLQHVEAVQRSGLRLQTID
                     SDRHIQLRASTEPDAVRGAELVLFSVKSTDTEAAGKALAPHLERDAAILTLQNGVDNA
                     ERLAATLGREVIPAVVYVAVEMAGAGHVRHHGRGELVIASSKQSGAIAAAFGAAGVPV
                     ETSDNVAGALWAKLIVNCAYNALSAITQLPYGRLVQGAGMPAVMRDVVDECLAVAQAA
                     GVNVPGDMHEAVRRIAQTMPGQYSSTAQDLTRAKKTEIDHLNGFVVRKGESLGVRTPV
                     NRVLLALVKTLESKS"
     gene            complement(1069..2034)
                     /locus_tag="VIG70_00715"
     CDS             complement(1069..2034)
                     /locus_tag="VIG70_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY8249100.1"
                     /translation="MKAVDHTFADVELPKASPSGRDLLVKVEAVSVNPVDTKQRKIKT
                     SGSHVLGWDAAGTVESIAKDVTLFKPGDAVYYAGDVTRPGCDSEFHLVDERIVGRKPK
                     KLDFAQAAAMPLTSITAWEAFHDRMKVQPGKSIIIIGGAGGVGSIGIQLAKISNLTVI
                     ATASRPQSIAWVKELGADHVVDHRKPLPPQIAALGLKHVDYIANFRGELDAYWEAMAE
                     LVAPQGALVAIVGNTKPLPMDVVRSKSATVCWELMFTRPRFQTPDMIEQHRLLNKVGE
                     WLDAGKLRGTLKETLSPINGANLRKAHEKLESGTMIGKLVLKGWG"
     gene            complement(2031..2678)
                     /locus_tag="VIG70_00720"
     CDS             complement(2031..2678)
                     /locus_tag="VIG70_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY8249101.1"
                     /translation="MPSRAVLFDFDGTLADTAPDLAAAVNRMLRDQGHEPLPLERLRP
                     FASAGARGLVHAAFGVKPDDPEYKALRESFLGFYGERVCRDTKLFPGVAELLRELGAR
                     EIRWGIVTNKATRFTEQIVSALKLTPDCVACGDTTPYLKPHPASLLHAAEQLGLAPAS
                     CCYLGDDLRDMKAARAAGMRPIAVEWGYHHPESGGPGTWEADAVIAHPMDLIRHL"
     gene            complement(2671..3366)
                     /gene="ubiG"
                     /locus_tag="VIG70_00725"
     CDS             complement(2671..3366)
                     /gene="ubiG"
                     /locus_tag="VIG70_00725"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEY8249102.1"
                     /translation="MNADPAELEKFSNLAHRWWDPEGEFRPLHDINPLRLDWIAGHAA
                     LEGATVLDVGCGGGILAEAMARRGARVTGIDLSEKALRVAQLHLHESRLSIEYLSVSV
                     EGHAATHAGEFDVVTCMELLEHVPDPASMVAACARLVRPGGQVFFSTINRNPKSYLFA
                     VVGAEYVLRLLPKGTHDYMRFIKPSELARWCRAAGLRPEELIGMTYNPITRRYRLGSD
                     CDVNYLQRCARDA"
     gene            complement(3426..3977)
                     /locus_tag="VIG70_00730"
     CDS             complement(3426..3977)
                     /locus_tag="VIG70_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8249103.1"
                     /translation="MIKNVWILLVALAFAAGCATEPAQPEPKPAPPPAPKPAPPPAPK
                     PAPPAPAPEKPKPAPEKPKPVAEKVTFAADVLFDFDKAVIKPEGKSKLDDISNKVRGI
                     NLEVVIAIGHADSIGSDAYNQRLSVRRSESVKAYLVSKGIEPNRVYTEGKGEKQPVAD
                     NKTRDGRAKNRRVEIEVIGTRKN"
     gene            complement(4010..5380)
                     /locus_tag="VIG70_00735"
     CDS             complement(4010..5380)
                     /locus_tag="VIG70_00735"
                     /inference="COORDINATES: protein motif:HMM:NF006549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HEY8249104.1"
                     /translation="MPEPQPARRSRGPGHAKISGPFLVSARWVVPVEPAGAIFEHHAV
                     AVRGGVIEQLLPLAEAARQFPDYERVELPEHVLIPGLVNAHTHAAMSLMRGMADDLPL
                     MRWLQEHIWPAETKHVSPRFVRDGTALACAEMLRGGITCFNDMYFFPEAALEAALEAG
                     MRVALGLIVIDFPTVYASDPDDYLRKGLALRDRSREQPLVSFCFAPHAPYTVSDPAFR
                     QVATLAAELDLPVHLHLHETEHEIERSLAEHGARPLERLRRLGLVGPGLIAVHAVHLE
                     PEEIHLLAHHGCSVAHCPSSNLKLASGFAPVDAMLKSGINVALGTDGAASNNRLDLFM
                     EMRTAALLAKAVARDAQAMPAYAALRAATLGGARALGLAERIGSIEAGKRADLVAVAL
                     RAPELAPCYDPVSHLVYAAGREHVTHVWVDGELRVRDGELRTGAPSLDTRWHIWQNAL
                     EGHADS"
     gene            complement(5326..5907)
                     /locus_tag="VIG70_00740"
     CDS             complement(5326..5907)
                     /locus_tag="VIG70_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8249105.1"
                     /translation="MKPQRLVLIAAALAFIGACATEPEKPAPAPAPAPAPAPVASPKP
                     VPAPPPPAPVAAKPEPKPEPPKKPAVTNLASTELFEFNKAVLTQEARSKLDNEVVAKL
                     RDLKDVRYIIVNGHADRLGSPQYNQRLSEKRADAVRAYLVSKGVDAAKVETLGFGKTL
                     PVKSCPDQKDRKGLIDCLSPNRRVVVEVQGTPR"
     gene            6028..8667
                     /gene="gyrA"
                     /locus_tag="VIG70_00745"
     CDS             6028..8667
                     /gene="gyrA"
                     /locus_tag="VIG70_00745"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY8249106.1"
                     /translation="MDQFAKETLPISLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMNEANNVWNRPYVKCARIVGEVLGKYHPHGDAATYEALVRMAQDFSMRYPL
                     IDGQGNFGSVDGDSAAAYRYTECRLEKIAAEMLVDIDKETVDFVPNYDGKEREPAVLP
                     TRAPNLLVNGSSGIAVGMATNIPPHNLGEVVDACLHVLAQPHCAIEEVIKLMPAPDFP
                     TSGIIYGLAGVHEGYRTGRGRVVMRARTHFEEIGKGDRHAVIVDELPYQVNKRALLER
                     MAEMVTEKRLEGISDIRDESDKDGMRVVIELKRGELPEVVLNNLYKQTQLQDTFGMNM
                     VALVDGQPRVLSLKELLESFISHRREVVTRRTVYELRKARERGHVLEGLAVALSNVDE
                     VIDLIKKAPSPADAKRGLMGRAWGSDLVKALIGSTSVAQFRPQGLSDLYGLKDDGYHL
                     SDEQAQAILELRLQRLTALEQDKIRDEYREVIVAITDLLDILAKPARITKIIGEELRK
                     LREEFADKRKSEIVTVAEDISIEDLIAPQDMVVTFSHGGYVKSQPLTDYRAQRRGGRG
                     KTATTMKEDDFIERLFVAHSHDYLLCFSNRGRLYWLKVYEVPAGSRSARGKPIVNMFP
                     LEEGEKITAVVPVKEFDENHYVFMSTAHGTVKKTPLAEFSRPRPSGIIAVGLEEGDYL
                     VGAALTDGKYDVMLFSSDGKAVRFEEGDVRPMGRQATGVRGMRLGAEQRVVCMLAAKD
                     ESRAVLTATANGYGKRTSVAEYPRHGRGGQGVIAIQVSERNGAVVGAVLVEEHDQVML
                     ISTGGVLIRTAVSQIREMGRSTQGVTLISLDAGERLAGVERIEERDLGGNGNGGGNGG
                     NEGGNGDGHEELPPPESPPPTVH"
     gene            8667..9740
                     /gene="serC"
                     /locus_tag="VIG70_00750"
     CDS             8667..9740
                     /gene="serC"
                     /locus_tag="VIG70_00750"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEY8249107.1"
                     /translation="MSRIFNFSAGPAMLPAEVLARAGDEMLDWRGSGMSVMEMSHRGP
                     EFMSIAAEAEADLRSLLAVPAGYKVLFLQGGATLQFAQVPMNLLRGKGKADYVVTGEW
                     SKKAVKEAKNYCDVAIAASSEDRNFTYAPKNFSVRKDAAYLHYCSNETIGGVEYHSIP
                     KAEIPLVADASSHFLSRPLEVSKFGLIYAGAQKNAGPAGLTIVLVREDLIGQAAKGTP
                     TVMDYKLQADADSMLNTPATYSMYVAGLVFKWLKQLGGLAEIENRNVAKAKALYDYLD
                     SSAFFRNPVAKEDRSRMNIPFTLKDKSLDEAFLKGAAERGLTQLKGHRSVGGMRASIY
                     NAMPLEGVQALVGYMRDFEKKHG"
     gene            9733..10926
                     /locus_tag="VIG70_00755"
     CDS             9733..10926
                     /locus_tag="VIG70_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HEY8249108.1"
                     /translation="MAKAHAAKREILVINQIAQVGLKRFPPEKYAVVKEANDPEAILV
                     RSQDLHKFEFGPSLRAVGRAGAGTNNIPVAALSRRGVPVFNAPGANANAVKELVLAGM
                     LIAARNLAPALEFVATQKNSSDLERKIEDGKKQFAGFELPGHTLGVIGLGKIGSLVAD
                     AAIRLGMNVLGYDPHITVEAAWSLPSQVKRAGSVDEVLKSSHFVSLHVPLLEKTRHLV
                     NAKNIEHMRAGSVLLNFSRDGVVAEDAVTQGLASGRLKWYVTDFPSPALIGAPGVIAL
                     PHLGASTGEAEDNSAVMVVDQLRDYLEHGNLQHAVNFPDAAMPREAPYRLAIANANVP
                     DMLGSISHALGRRKINIHNMLNKSKGEMAYTLVDTDTSVTAEALKELAGLNGVLAVRY
                     LPVDG"
     gene            10919..11986
                     /gene="pheA"
                     /locus_tag="VIG70_00760"
     CDS             10919..11986
                     /gene="pheA"
                     /locus_tag="VIG70_00760"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY8249109.1"
                     /translation="MADEIQKLRAEIDRVDDEVAALLQRRAGLAQKIGALKQGAGAYR
                     PEREAEILNRLSAKAGILAADRVAAVFREIISACRGLEEAIRVSYLGPEGTFSEQAVR
                     KHFGRAVEALPAASVDEAFRRCESGSAQFTVVPVENSTEGAVGRTLDLLLATPLRICG
                     EVELRVQQNLLSKASGLQAIRRIYSHAQSLAQCNGWLAQNLPSVERVPVTSNADAARR
                     AADEPAAAAIAGEAAAERYQLGVLARAIEDDPNNTTRFLVLGNVDPAPTGKDRTSIVL
                     SAENKPGAVHALLTPLAQHRVSMTRIESRPARARSALWEYMFFIDVEGHQKDETVARA
                     LAELKRLAPYLKVLGSYPAAN"
     gene            11996..13099
                     /gene="hisC"
                     /locus_tag="VIG70_00765"
     CDS             11996..13099
                     /gene="hisC"
                     /locus_tag="VIG70_00765"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY8249110.1"
                     /translation="MDPCELSPSYVRSIAPYQPGKPISELAREMGLDERNIVKLASNE
                     NPRGIGPRTRAAIEAALGDIARYPDGNGFELKQALARRYGVDMGSIVLGNGSNDVLEL
                     AALAFLGPGRAAVFSQHCFAVYPLATQARGARSIVVPAKNYGHDLEAMVKAIDDETTV
                     AWLANPNNPTGTFARHEEVEAFLKRVPERVLVVLDEAYNEYLVPDLRSDTFKLVKRHP
                     NLVVTRTFSKAYGLAGLRVGYALAHPSVADVMNRVRQPFNVNSIALAAATAALDDMEF
                     VARSYAENLQGLRQLEEGARGLGLKYIPSHGNFITLQVGKAPEIYKRLLRRGVIVRPV
                     GGGYGLPEHLRVTVGTAQENEKFLGALAASLKE"
     gene            13109..13957
                     /locus_tag="VIG70_00770"
     CDS             13109..13957
                     /locus_tag="VIG70_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEY8249111.1"
                     /translation="MKLAVVGVGLIGGSFALALKQAKLATQVVGVGRNAANLKLALER
                     GAIDSIAPDPATAARGADLILVATPVAQFPSVFASLADAKALITDGGSTKRDVIAAAR
                     KALGKKVAQFVPAHPIAGAENSGAGAASAELFRNRRVVLTPLRENSKPDIAKVEAAWS
                     ACGARVSRMDPEEHDAVLATVSHLPHVLAYALVHGVAKRNNSEQLFSFAAGGFRDFTR
                     IASSHPEMWRDICLANRDRVLQELKSYANELGSIRKLIQKGDAAGLEKVFAAAREARN
                     KWIRSS"
     gene            13939..15201
                     /gene="aroA"
                     /locus_tag="VIG70_00775"
     CDS             13939..15201
                     /gene="aroA"
                     /locus_tag="VIG70_00775"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY8249112.1"
                     /translation="MDSLELKPASRAAGTVRLPGSKSISNRVLLLAALAQGATELDGL
                     LDADDTRVMREALARLGVSFEGNTVKGVGGPFPVKQADLFLGNAGTAFRPLTAALAFS
                     GGEYRLSGVPRMHERPIGDLVDALRGIGARIDYLGKEGFPPLAVHPGRLSLEKLRVRG
                     DVSSQFLTALLMALPLAGKPARVEVQGELISRPYVEITLNVMKRFGIEVKRTGWRYFD
                     VPAGAYRSPGKIYVEGDASSASYFLAAGAIGGGPVRVEGVGRGSIQGDVRFTEVLERM
                     GARVSMEADAIEVSGEKKLKPVDMDMNHIPDAAMTAAVLALFADGPSTIRNVASWRVK
                     ETDRLAAMATELRKLGAQVEEGADFLKISPPAALKGNVLVDTYDDHRMAMSFSLAALG
                     GVPVRINDPQCVAKTFPEYFEVFSAIVA"
     gene            15357..15806
                     /locus_tag="VIG70_00780"
     CDS             15357..15806
                     /locus_tag="VIG70_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249113.1"
                     /translation="MLNKDFREFVELLGSAGVEYLIVGGYALAAHGHPRYTGDLDIWI
                     RSTPANVDRLLQALSGFGFGSLGLKREDFLQPSAVVQLGYPPSRIDLLTGLSGVDFDA
                     CYPNRAEITIAGVRLPFIGLAEFRANKRASGRAKDLADLESLDGKDS"
     gene            complement(15811..16773)
                     /locus_tag="VIG70_00785"
     CDS             complement(15811..16773)
                     /locus_tag="VIG70_00785"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8249114.1"
                     /translation="MLIAPHVVAQPAKKIHRVGFVATTSPLAEISGPDPANPFVRAFV
                     HGLRDLGYVQGKNLVLEMRTLEGKPERLQVLMADFVRLKMEVVFLPSSLLVPPAHKAA
                     PTLPIVGLVNAGHLISTGLAQSMGRPGGTITGLSLDVDEDLEAKRVELFAELVPRARR
                     IAFVGLREEWERPYAVKMRAAAERRGKTMVLVESAPGDFAPAFSRLKQEGVDAYMVER
                     SPRAYGRRHEIGQLVVASGLPGSCGQAELVEQGCLMSYSPDNNDLARRLSVYVDKILK
                     GAKPGDLPIEAPTKFELTINAKTAKALGLAIPQSVLLRADRVIE"
     gene            16848..17528
                     /gene="cmk"
                     /locus_tag="VIG70_00790"
     CDS             16848..17528
                     /gene="cmk"
                     /locus_tag="VIG70_00790"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY8249115.1"
                     /translation="MSAHIPVIAIDGPSASGKGTIAKRVAGALGFHYLESGALYRLVA
                     LVALRQGLQDEAAIADAAEHMDVVFQEDEILLEDQDVSAQIRHETVGSRASEIAPMRK
                     VRAALLTRQRAFRQPPGLVADGRDMGTIVFPDAALKIFLTAGPEIRAQRRYKQLIEKG
                     IAANLGALSRDLAERDKRDATRAVAPLVPAPDSQVLDSSALSIEEGVDRILELWAGGP
                     RGGARFAT"
     gene            17573..>18030
                     /locus_tag="VIG70_00795"
     CDS             17573..>18030
                     /locus_tag="VIG70_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY8249116.1"
                     /translation="MASVSPQPSKSPASESFAALFEESLNRKEMRVGEVITAEVVSID
                     DNFVVVNAGLKSESTIAIEEFRTDAGQVDVKVGDFVSVSIEALEDGYGSTRLSRDKAK
                     RMAAWIGLEKALESGEKIKGVITGKVKGGLTVMANGIRAFLPGSLVDLRPV"
BASE COUNT         3029 a         5869 c         6146 g         2986 t
ORIGIN      
        1 taggcggcta gagcgaaacg ctcgtcgttg gcgtttgtag tgtttccaga atggatttac
       61 gaggtgatct ggtcctcggc atgccctgcg ctgcttcgat gcccacgtcg aaaccaggtc
      121 gcccccggaa tcgtgtgttg ctgcgcattg tcccatatgg ggcctgcggc ggcctcattc
      181 aagacttgct ttccagggtt ttgaccagcg cgagcagcac ccggttcacc ggcgtgcgca
      241 cgccgagcga ctcgcccttg cgcaccacga acccgttgag atggtcgatc tcggttttct
      301 tcgcgcgcgt gagatcctgc gcggtcgagg agtattggcc cggcatggtt tgggcgatgc
      361 ggcgcacggc ttcgtgcatg tctccgggca cgttcacccc ggcggcttgc gcgaccgcga
      421 ggcactcgtc gacgacgtcg cgcatgacgg ccggcatgcc ggcgccttgc accaaccgcc
      481 catagggaag ctgtgtgatt gccgagagcg cgttgtaggc gcagttgacg atgagcttcg
      541 cccagagcgc gccggcgacg ttatccgagg tctccaccgg cacgccggcc gcgccgaaag
      601 cagccgcgat cgccccgctt tgtttgctgg atgctatgac taattctccc cggccgtgat
      661 gccgcacatg acccgccccc gccatctcga ccgccacgta gaccaccgcg gggatgacct
      721 cgcggccgag cgtcgccgcc agccgctccg cattgtcgac gccgttctgc agcgtgagga
      781 tggcggcgtc gcgctcgagg tggggcgcga gcgccttgcc ggcggcttcg gtgtcggtcg
      841 atttcacgga gaacaggacg agctcggcgc cgcgcacggc gtccggctcg gtgctcgccc
      901 tcagctgaat gtgtcggtca gaatcaatgg tctgcagccg cagtcccgaa cgttgcacgg
      961 cttccacatg ctgcagcctg ccgatcaggg ttacctcgtg cccggcgcgg gcgaggacgg
     1021 caccgtagta gcagccgacg gcgccggcgc ccatcacggc aacccgcttc agccccaccc
     1081 tttcagaacc agcttgccga tcatggttcc cgactcgagc ttctcgtgcg ccttcctcag
     1141 gttggcgccg ttgatcggcg aaagcgtctc cttcagcgtg cctcgcagct tgccggcgtc
     1201 gagccactcg ccgactttat tcaggagccg gtgctgctcg atcatgtccg gcgtctggaa
     1261 gcgggggcgc gtgaacatga gctcccagca gaccgtcgcg ctcttcgagc gcacgacgtc
     1321 catcggcagc ggcttggtgt tgcccacgat cgcgacgagg gcgccctgcg gcgcgaccag
     1381 ctcggccatg gcttcccagt aggcgtcgag ctcgccgcgg aagttggcga tgtagtcgac
     1441 gtgcttgaga ccgagcgcgg cgatctgcgg cgggagcggc ttgcgatggt cgaccacgtg
     1501 gtccgcgccc agctctttca cccatgcgat cgactgcggg cgagaggcgg tggcgatcac
     1561 ggtcaagttc gagattttgg cgagctggat gccgatcgag cccacgccgc ccgccccgcc
     1621 gatgatgatg atggacttgc cgggctggac cttcatccgg tcgtggaagg cctcccaggc
     1681 ggtgatcgag gtgagcggca tcgccgcggc ctgcgcgaag tcgagcttct tcggcttgcg
     1741 cccgacgatg cgctcgtcga cgaggtggaa ctcgctgtcg cacccgggcc gcgtcacgtc
     1801 gccggcgtag tagaccgcat cgcctggctt gaacagcgtt acatccttgg caatggattc
     1861 cactgtgcct gcagcgtccc atccgagcac atgcgagccc gaggtcttga ttttgcgttg
     1921 cttggtatcc accgggttga cggagaccgc ttcgaccttc accagcaggt cgcgtccgga
     1981 aggagaggcc ttgggcagct cgacgtcggc aaaggtgtga tcgacggctt tcataggtgt
     2041 ctgatgaggt ccatgggatg agcgatgacc gcgtcggctt cccaggtgcc ggggccgccg
     2101 ctctcgggat ggtgatagcc ccattcgacc gcgatcggcc gcatgccggc cgcgcgcgcg
     2161 gccttcatgt cgcgcaggtc gtcgccgagg tagcagcaac tggccggcgc cagcccgagc
     2221 tgctcggcgg catggagcag cgatgccggg tggggcttga ggtaaggcgt ggtgtcgccg
     2281 caagccacgc agtcgggcgt cagtttgagc gcagaaacga tctgctcggt aaatcgcgtc
     2341 gccttgttcg tgacgatgcc ccagcggatt tcgcgcgcgc ccagctcgcg caggagctct
     2401 gcgacgccgg gaaagagctt cgtgtcgcgg cagacgcgct cgccatagaa gccgagaaaa
     2461 gactcgcgca gcgccttgta ctccgggtcg tcgggcttga cgccgaacgc cgcgtgcacc
     2521 agcccgcgcg cgcccgccga ggcgaacggg cgcaggcgct cgaggggcag cggctcgtgg
     2581 ccctggtccc tgagcatgcg gttcaccgcg gcggcgagat ccggcgcggt gtcggcgagc
     2641 gtgccgtcga aatcgaagag gacggcccgg ctaggcatcg cgcgcgcagc gctgcaggta
     2701 gttcacgtcg cagtcgcttc ccaggcggta gcggcgagtg atcgggttgt aggtcatgcc
     2761 gatcagctcc tccgggcgca gccccgccgc gcggcaccag cgcgcgagct cggagggctt
     2821 gatgaagcgc atgtagtcgt gggtcccctt cggcagcagg cgcagcacat actcggcgcc
     2881 gacgaccgcg aaaagatagg acttggggtt gcggttgatg gtcgagaaaa agacctggcc
     2941 tcccgggcgc accaggcgag cgcaggcggc gaccatgctt gccgggtcgg gcacgtgctc
     3001 gaggagctcc atgcaggtga cgacgtcgaa ctcgccggca tgggtggccg catgaccctc
     3061 gacgctcacg gagaggtatt cgattgaaag acggctttca tgaagatgaa gctgggccac
     3121 gcgcagcgcc ttctccgaca ggtcgatgcc ggtgacgcgc gcgccgcgcc gcgccatcgc
     3181 ctcggcgagg atgccgccgc cgcagccgac gtcgagcacg gttgcgccct cgagcgccgc
     3241 gtgcccggcg atccagtcca ggcgcagcgg attgatgtcg tgcagcggcc ggaactcgcc
     3301 ctcgggatcc caccagcggt gggcgagatt gctgaatttc tcgagctcgg cggggtctgc
     3361 gttcacacgg gtattaaaac aaaaaacccc gccgaagcgg ggttttctgc cgaggcgaag
     3421 accggctagt tcttgcgcgt gccgatgact tcgatctcga cgcggcggtt cttggcgcgg
     3481 ccgtcgcggg tcttgttgtc ggcaaccggc tgcttctcgc ccttgccttc ggtgtagacg
     3541 cggttgggct cgatgccttt cgacaccagg tacgccttca ccgattcgga gcgtcgcacc
     3601 gacagcctct ggttgtacgc gtcggagccg atcgagtcgg cgtggccgat cgcgatcacc
     3661 acttcgaggt tgatgccgcg caccttgttc gagatgtcgt cgagcttcga cttgccctcg
     3721 ggcttgatca ccgccttgtc gaagtcgaac agcacgtcgg cggcgaaggt caccttttcg
     3781 gcgaccggct tgggcttctc cggcgcgggc ttgggcttct ccggcgcagg agcgggtgga
     3841 gcgggcttcg gcgccggcgg cggcgcgggc ttcggcgccg gcggcggtgc gggtttgggt
     3901 tcgggctgcg ccggttcggt cgcgcatcca gccgcgaacg cgagcgcgac gagaaggatc
     3961 cagacgtttt tgatcatttt tcctcccgct ggtcgaagtc ttttttggat caagagtctg
     4021 cgtgaccctc gagggcattc tgccatatgt gccaacgggt gtccaagctg ggagcaccgg
     4081 ttcttagctc tccgtcgcgc acgcgcaact cgccgtcgac ccagacgtgc gtgacgtgct
     4141 cgcggcccgc ggcatagacg agatgcgaga ccgggtcgta gcagggtgcg agctccggcg
     4201 cgcgcagcgc caccgcgacc aggtcggcgc gcttgccggc ctcgatcgag ccgatgcgct
     4261 cggccaggcc cagggcgcgg gcgccgccga gcgtcgcggc gcgcaacgcc gcgtaggcgg
     4321 gcatggcttg cgcatcgcgc gccacggcct tggcgagcag cgccgcggtg cgcatttcca
     4381 tgaacaggtc caggcgattg ttgctcgcag cgccgtcggt tccgagcgcc acgttgatgc
     4441 cgcttttcag catggcatcg accggggcga acccgctggc gagcttgagg ttggacgacg
     4501 ggcaatgcgc taccgagcag ccatgatgcg caaggagatg aatctcttca ggttccaggt
     4561 gaacggcatg gacggcgatc aggccggggc cgaccagccc gaggcggcgc aggcgttcga
     4621 gcggccgcgc gccgtgttcg gcaagcgagc gctcgatctc gtgctcggtc tcgtgcaggt
     4681 ggaggtgcac cggcaggtcg agctccgcgg cgagcgtcgc cacctgccgg aacgcagggt
     4741 ccgagaccgt gtagggcgcg tgcggcgcga agcagaagga cacgagcggc tgctcccggc
     4801 tgcgatcgcg caacgcgagc cccttgcgca ggtagtcgtc gggatcggat gcatagacgg
     4861 tggggaagtc gatcacgatc agcccgagcg cgacgcgcat gccggcttcg agcgccgcct
     4921 cgagcgcggc ctccgggaag aaatacatgt cgttgaagca ggtgatcccg ccgcgcagca
     4981 tttccgcgca ggcgagcgcc gtgccgtcgc gcacgaagcg cggcgagacg tgtttcgttt
     5041 ccgccggcca gatgtgctcc tgcagccagc gcatcagggg caggtcgtcg gccatgccgc
     5101 gcatcagtga catggccgcg tgggtgtgcg cgttgacgag ccctggaata aggacgtgct
     5161 ccgggagctc tacgcgttcg tagtcgggga actgccttgc agcctctgcc aacggaagaa
     5221 gctgctcgat gacgccaccg cgcaccgcga cggcatgatg ctcgaagatc gcgcccgccg
     5281 gctcgaccgg tacgacccag cgggcgctga cgaggaaagg gccgcttatc ttggcgtgcc
     5341 ctggacctcg actacgacgc gccggttggg gctcaggcaa tcgatcaggc ccttgcggtc
     5401 cttctggtcc gggcaggact tgaccggaag cgtcttgccg aagccgagag tttcaacctt
     5461 cgccgcgtcc acgcccttcg acaccaggta cgcgcgcacc gcgtcggcgc gcttctcgga
     5521 caggcgctgg ttgtactgcg gcgatcccag ccggtccgcg tggccgttga cgatgatgta
     5581 gcgcacgtcc ttcaggtcgc gcagcttggc gaccacttcg ttgtcgagct tcgagcgcgc
     5641 ttcctgggta agcaccgcct tgttgaactc gaagagctcg gtcgatgcga gattcgtcac
     5701 cgcgggcttc ttcggcggct cgggcttcgg ttccggcttc gcggccaccg gcgccggcgg
     5761 cggcggggcc ggcacgggct tcggcgaggc tacgggcgcg ggtgccggtg ccggagcggg
     5821 cgccggtgcg ggtttctccg gttcggtggc gcaggcgccg atgaaagcga gcgcggcggc
     5881 gatcagaacg agcctctgcg gtttcatttt cctcccggtt ttttgtcgtc tcgagacgag
     5941 cgaatttaag cacaggaaaa cgttcccgga caagctgttt ttagagggaa aaccgatgct
     6001 tgcatgataa aatttgagcc cttcccgatg gaccaattcg ccaaagaaac gctgccgatc
     6061 tcgctcgaag aggagatgcg gcgctcctac ctcgactacg cgatgagcgt gatcgtcggg
     6121 cgtgcgcttc ccgacgtccg ggacggcctg aaaccggtcc accggcgggt gctgttcgcc
     6181 atgaacgagg cgaacaacgt gtggaaccgg ccctacgtga aatgcgcgcg catcgtcggc
     6241 gaggtgctcg gcaagtacca cccgcacggc gacgcggcga cctacgaggc cctggtgcgc
     6301 atggcccagg acttctcgat gcgctatccg ctgatcgacg gccagggcaa tttcggctcg
     6361 gtcgacggcg attccgccgc ggcctatcgc tacaccgaat gccggctgga aaagatcgcc
     6421 gccgagatgc tggtcgacat cgacaaggag acggtcgact tcgtgccgaa ctacgacggc
     6481 aaggagcgcg agcctgcggt gctgccgacg cgcgcgccga acctcctggt caacggctct
     6541 tcgggcatcg ccgtcggcat ggcgaccaac atcccgccgc acaacctggg cgaggtggtc
     6601 gacgcctgcc tccacgtcct cgcgcagccg cactgcgcga tcgaggaggt gatcaagctc
     6661 atgccggcgc cggatttccc gacctcggga atcatctacg ggcttgccgg cgtgcacgag
     6721 ggctaccgta ccggccgcgg ccgcgtggtg atgcgcgccc gcacgcattt cgaggagatc
     6781 ggcaagggcg accggcacgc cgtcatcgtc gacgagctgc cctaccaggt gaacaagcgc
     6841 gcgctgctcg agcgcatggc ggagatggtc accgagaaga ggctcgaggg catctccgac
     6901 atccgcgacg agtccgacaa ggatggcatg cgcgtggtga tcgagctcaa gcgcggcgag
     6961 ctgccggaag tggtgctgaa caacctctac aagcagacgc agctccagga caccttcggc
     7021 atgaacatgg tggcgctggt cgacggccag ccgcgcgtgc tgtcgctgaa ggagctgctc
     7081 gagtcgttca tctcccaccg ccgcgaggtg gtgacgcgcc gcacggtcta cgagctgagg
     7141 aaggcgcgcg agcgcggcca cgtgctcgaa ggcctggccg tggcactgtc gaacgtcgac
     7201 gaggtgatcg acctgatcaa gaaagcgccg agccctgccg atgccaagcg cggcctcatg
     7261 ggacgcgcct ggggctccga cctcgtgaag gccttgattg gcagcacctc ggtggcgcag
     7321 ttccgtcctc agggactgag cgatctctac gggttgaagg acgacggcta ccacctctcc
     7381 gacgagcagg cgcaagcgat cctcgagctg cggctgcagc gcctcacggc gctcgagcag
     7441 gacaagatcc gcgacgagta ccgcgaagtg atcgttgcga ttaccgatct cctggacatc
     7501 ctcgcgaagc ccgcccgcat cacgaagatc atcggcgagg agctgcgcaa gctgcgggag
     7561 gaattcgccg acaagcgcaa gagcgagatc gtcaccgtcg cagaggatat ttcaatcgag
     7621 gacctgatcg cgccgcagga catggtggtg acgttctcgc acggcggcta cgtcaagtcg
     7681 cagccgctca ccgactaccg ggcacaaaga cggggcggcc gggggaagac cgcgaccacg
     7741 atgaaggaag acgacttcat cgagcgcctg ttcgtcgccc actcgcacga ctacctgctg
     7801 tgcttctcga accgcggccg gctctactgg ctcaaggttt acgaggtgcc ggccggcagc
     7861 cgctcggcgc gcggcaagcc gatcgtcaac atgttcccgc tcgaggaggg cgagaagatc
     7921 acggcggtcg tgccggtgaa ggagttcgac gagaatcatt atgtgttcat gtcgaccgcg
     7981 catggcacgg tgaagaagac cccgctcgcc gaattctcgc gcccgcgccc gagcggcatc
     8041 atcgccgtcg gcctggagga gggcgactac ctggtcggcg ccgcgctcac cgacgggaag
     8101 tacgacgtga tgctcttttc ctcggacggc aaggcggtgc gcttcgagga aggcgacgtg
     8161 cgcccgatgg gccgccaggc gaccggcgtg cgcgggatgc gcctgggtgc ggagcagcgc
     8221 gtggtctgca tgctggcggc gaaggacgaa tcgcgcgcgg tgcttaccgc cacggccaac
     8281 ggatatggca agcgcacctc ggtcgccgaa tacccgcgcc acggtcgcgg cggccagggc
     8341 gtgatcgcga tccaggtgtc cgagcgcaac ggcgcggtcg tcggcgcggt gctggtcgag
     8401 gagcacgacc aggtgatgct gatctcgacc ggcggcgttc tgatccgcac cgcggtatcg
     8461 cagatccgcg agatgggccg ctcgacccag ggcgtgaccc tgatctcgct cgacgcgggc
     8521 gagaggctcg ccggcgtgga gcgcatcgag gaacgcgacc tcggcggcaa cggcaacggc
     8581 ggtggcaacg gaggaaacga gggcggaaat ggggatggcc acgaggaatt gccgccgccg
     8641 gagtcgccgc cgcccacagt ccattgatgt cccgcatctt caatttcagc gcggggcccg
     8701 cgatgctccc cgcggaagtc ctcgcacgcg ccggcgacga gatgctcgac tggcgcggca
     8761 gcggcatgtc ggtgatggaa atgagccacc gcggccccga gttcatgtcg atcgctgccg
     8821 aggccgaggc cgacctgcgc tcgctgctcg ccgtgccggc gggctacaag gtgctgttcc
     8881 tgcagggcgg cgcgaccctg cagttcgccc aggtgccgat gaacctgctg cgcggcaagg
     8941 gcaaggccga ctatgtcgtg accggcgagt ggtcgaagaa ggcggtgaag gaggcgaaga
     9001 actactgcga cgttgcgatc gccgcgagct ccgaggaccg caacttcact tacgcgccca
     9061 agaatttctc cgtgaggaag gacgcggcct acctgcacta ttgctcgaac gagacgatcg
     9121 gcggcgtcga ataccattcg attcccaagg cggaaatacc cctggtcgcg gacgcctctt
     9181 cgcacttcct gtcgcgcccg ctcgaggtgt cgaagttcgg gctgatctac gccggcgcgc
     9241 agaagaacgc gggacccgcg ggcctcacca tcgtcctcgt gcgcgaggac ctgatcggcc
     9301 aggcggcgaa aggcacgccg acggtcatgg actacaagct gcaggcggac gccgattcga
     9361 tgctgaatac gccggccacc tattcgatgt atgtcgccgg cctggtgttc aagtggctga
     9421 agcagctcgg cggtctggcc gaaatcgaga ataggaacgt cgcgaaggcg aaagcgctgt
     9481 acgactacct cgattcgagc gcctttttca ggaatccagt cgcgaaggaa gaccgctcgc
     9541 ggatgaacat tcccttcacg ttgaaggaca agtctctgga tgaagctttc ctgaagggcg
     9601 cggccgagcg cgggcttacc cagctcaagg gccaccgttc cgtcggcggc atgcgcgcgt
     9661 cgatctacaa cgcgatgccg ctcgagggtg tgcaagccct tgtcggctat atgcgcgact
     9721 tcgagaagaa gcatggctaa ggcgcacgcg gcgaagcgcg agatcctggt gatcaaccag
     9781 atcgcccagg tcggcctgaa gcgctttccc ccggagaagt acgcggtggt caaggaggcg
     9841 aacgatcccg aggcgatcct ggtgcgctcc caggacctgc acaagttcga gttcggcccc
     9901 tcgctgcggg ccgtgggccg tgccggcgcc ggcaccaaca acattccggt cgctgcgctc
     9961 tccaggcgcg gcgtgccggt gttcaatgcg ccgggcgcca acgccaacgc ggtgaaggag
    10021 ctggtgctcg cggggatgct catcgccgcg cgcaacctcg cgccggccct cgaattcgtc
    10081 gctacgcaga agaattcttc cgacctggaa aggaagatcg aagacgggaa aaagcagttc
    10141 gccggcttcg agctccccgg ccataccctg ggcgtgatcg gcctgggcaa gatcggcagc
    10201 ctggtcgccg acgcggcgat ccgcctgggc atgaacgtgc tgggctacga cccgcacatt
    10261 accgtcgagg ccgcctggag cctgccctcg caggtcaagc gcgccggctc ggtcgacgag
    10321 gtgctgaagt cgagccactt cgtcagcctg cacgtgccgc tcctggaaaa gacccggcac
    10381 ctcgtcaacg cgaagaacat cgagcacatg cgcgccggct cggtgctgct caacttctcg
    10441 cgcgatggcg tggtggccga agacgcggtg acgcagggcc tcgcgagcgg ccgcctgaag
    10501 tggtacgtga ccgatttccc gagccctgcg ctgattggcg cgcccggcgt gatcgcgctg
    10561 ccgcacctcg gcgcatcgac cggcgaggcc gaagacaact ccgccgtgat ggtcgtcgac
    10621 cagctgcggg actacctcga gcacggcaac ctgcagcatg cggtcaactt ccccgacgcg
    10681 gcgatgccgc gcgaggcgcc ctaccggctc gccatcgcca acgccaacgt gcccgacatg
    10741 ctcggcagca tctcgcacgc gctcgggcgc cgaaagatca acatccacaa catgctgaac
    10801 aagtccaagg gcgagatggc gtatacgctg gtcgataccg atacttcggt gaccgccgag
    10861 gcgctcaagg agcttgctgg cctgaacggg gtcctcgcgg tacggtatct tccggtggat
    10921 ggctgacgag atccaaaaac tgcgcgctga aatcgaccgc gtcgacgacg aggtcgcggc
    10981 gctgttgcag cgccgggcgg ggctggcgca gaaaatcggc gcgctgaagc agggcgcggg
    11041 cgcctaccgg ccggagcgcg aggccgaaat cctgaacagg ctctcggcga aggcgggcat
    11101 cctcgcggcc gatcgcgtcg ccgcggtgtt ccgcgagatc atctcggcgt gccgcgggct
    11161 ggaggaggcg atccgcgtct cctacctcgg gccggaaggc acgttcagcg agcaggcggt
    11221 gcgcaagcac ttcggccgtg cggtggaggc gctgccggcg gcatcggtcg acgaggcgtt
    11281 tcgccgctgc gagtcaggct cggcccagtt caccgtggtg ccggtcgaga attccacgga
    11341 gggcgcagtg ggccgcacgc tcgatctctt gctcgccacg ccgcttcgca tctgcggcga
    11401 ggtcgagctt cgggtccagc aaaatttgct ttccaaggcg agcggccttc aggccatccg
    11461 ccgcatctat tcccacgccc agtcgctggc gcaatgcaac ggctggctgg cgcaaaacct
    11521 gcccagcgtc gaaagagtgc cggtgacctc gaatgccgac gctgcgcggc gcgcagccga
    11581 cgagcccgcg gcggcggcga tcgccggcga ggcggcggcc gagcgctacc agctcggcgt
    11641 gctcgcgcgc gcgatcgagg acgatccgaa caacaccacg cgctttctcg tcctcggcaa
    11701 tgtcgatccc gcgcccaccg gcaaggaccg cacctcgatc gtcctttcgg ccgagaacaa
    11761 gcccggcgcg gtgcacgcgc ttctcacgcc gcttgcccag caccgcgtca gcatgacgcg
    11821 catcgagtcg cgccccgcgc gggcgcgatc ggcgctttgg gaatacatgt tcttcatcga
    11881 cgtcgaaggg caccagaagg acgaaaccgt ggcgagggcg ctggctgagc tgaagcgcct
    11941 ggccccttac ctaaaggtgc tgggctccta cccggcagca aactagaaaa tgttgatgga
    12001 tccctgcgag ctgtcgcctt cctatgtgcg ctcgatcgcg ccttaccagc cgggcaagcc
    12061 gatctcggag ctcgcgcgcg agatgggtct cgacgagcgc aacatcgtga agctcgcctc
    12121 caacgagaac ccgcgcggca tcggcccgcg cacccgcgcc gcgatcgagg cggcgctcgg
    12181 cgacatcgcg cgctatcccg acggcaacgg cttcgagctg aagcaggcgc tcgcccggcg
    12241 ctacggcgtc gacatgggat cgatcgtgct cggcaacggc tcgaacgacg tgctcgagct
    12301 cgctgcgctc gccttcctcg ggccgggccg cgccgcggtg ttctcgcagc actgcttcgc
    12361 cgtgtaccct ctcgccactc aggcgcgcgg cgcgcgctcg atcgtggtgc cggcgaagaa
    12421 ctacggccat gacctcgagg cgatggtgaa ggcgatcgac gacgagacca ccgtcgcgtg
    12481 gctcgccaac cccaacaacc cgaccgggac cttcgcgcgc cacgaggaag tggaagcgtt
    12541 cctcaagcgc gtgcccgagc gcgtcctggt ggtgctcgac gaggcctaca acgagtacct
    12601 ggtgcccgac ctgcgcagcg acacgttcaa gctcgtcaag cgccatccca acctggtcgt
    12661 cacgcgcacc ttctcgaagg cctacgggct cgccgggctg cgcgtcggct atgcgctggc
    12721 gcatccctcg gtcgccgacg tcatgaaccg ggtgcggcag cccttcaacg tcaacagcat
    12781 cgcgctcgcc gcggcgaccg cggcgctcga cgacatggag ttcgtggcgc gcagctacgc
    12841 cgagaacctg cagggactgc ggcagctcga agaaggcgcg cgcgggctcg gcctcaagta
    12901 catcccgtcg cacggcaact tcatcaccct acaggtggga aaggccccgg aaatctacaa
    12961 gcggttgctg cgccggggcg tgatcgtgcg cccggtgggc ggcggctacg ggctgcccga
    13021 gcacctgcgc gttaccgtgg gcacggctca ggagaacgag aagttcctgg gcgcgctcgc
    13081 tgccagcctc aaagaatgaa atgaggcaat gaagctcgcg gttgtcggcg tcggcctgat
    13141 cggcggctct ttcgcgctcg cgctgaagca ggccaagctc gcgacgcaag tcgtcggcgt
    13201 cggtcgcaac gcggcgaacc tgaagctcgc cctggagcgc ggcgcgatcg actcgattgc
    13261 gccggatccc gccactgcgg cgcgcggcgc ggatctgatc ctggtcgcga cgccggtggc
    13321 gcagtttccc tccgtatttg catcgttggc cgatgcaaag gcgctcatca ccgacggcgg
    13381 gagcacgaag cgcgacgtga tcgcggcggc gcgcaaggcg ctcggcaaga aggtcgcgca
    13441 attcgtgccc gcgcatccga tcgccggcgc cgagaacagc ggcgccggcg cggcgagcgc
    13501 ggagctattt cgcaaccggc gcgtcgtgct gacgccgctg cgggagaatt ccaagccgga
    13561 tatcgcgaaa gtcgaagcgg cgtggtctgc ctgcggtgcg cgcgtgtcgc gcatggaccc
    13621 cgaggagcac gacgccgtgc tcgcaacggt cagccacctg ccgcacgtcc tggcctacgc
    13681 gctggtgcac ggcgtcgcga agcgcaacaa ttccgagcag ctcttttcct tcgccgccgg
    13741 aggattccgc gacttcacgc gcatcgcctc gagccacccc gagatgtggc gcgacatctg
    13801 cctcgcgaac cgcgaccggg tgctgcagga actgaagtcc tacgcgaacg agctcggctc
    13861 gatccgcaag ctgatccaga aaggcgacgc ggccgggctg gaaaaggtct ttgccgccgc
    13921 gcgggaagcg cgcaacaagt ggattcgctc gagctgaagc ccgcgtcgcg tgccgcgggc
    13981 acggtgcggc tcccagggtc gaagagcatc tccaaccggg tcctcctgct cgccgcgctg
    14041 gcgcaaggcg cgaccgagct cgacggcctg ctcgacgccg acgacacgcg cgtgatgcgc
    14101 gaggcgctgg ctcgcctcgg tgtttctttc gaagggaata ccgtcaaggg cgtcggcggg
    14161 ccatttccgg tcaagcaggc cgacctgttt ctcggcaacg ccggcaccgc gttccggccg
    14221 ctcaccgcgg cgcttgcctt ctccggcggc gaataccgcc tttcgggagt gccgcgcatg
    14281 cacgagcgcc cgatcggcga cctggtggat gcgctgcgcg gcatcggcgc gcgcatcgac
    14341 tacctgggga aggaaggatt cccgccgctt gccgttcatc ccggcaggct ctctctggaa
    14401 aaactgcggg tgcggggaga cgtctcgtcg cagttcctca ccgcgctgct gatggcgctc
    14461 ccgcttgcgg gcaagccggc gcgcgtcgag gtgcagggcg agctgatctc caggccctac
    14521 gtcgagatca ccctaaacgt gatgaagcgc ttcggcatcg aggtgaagcg caccggctgg
    14581 cgctacttcg acgtccccgc gggcgcctat cgctcgccgg gaaagatcta cgtcgagggc
    14641 gacgcgtcct ccgcctccta tttcctcgcc gccggcgcga tcggcggcgg accggtgcgc
    14701 gtggaaggtg tcgggcgcgg cagcatccag ggcgacgtgc gtttcacgga agtgctggag
    14761 cggatggggg cccgggtttc catggaagca gatgccatcg aggtctccgg cgaaaaaaag
    14821 ctcaagcccg tcgacatgga catgaaccac attcccgacg ccgcgatgac cgccgccgtg
    14881 ctcgcgctgt tcgccgacgg gccgagcacg atccgcaacg tggcgagctg gcgcgtcaag
    14941 gaaaccgatc gtctggcggc gatggccacc gagctgcgca agctcggcgc tcaggtcgag
    15001 gagggtgcgg atttcttgaa gatttccccg ccggcggctc ttaaaggaaa tgttctggtc
    15061 gacacctacg acgatcaccg catggcgatg agcttctcgc tcgccgcgct cggcggcgtg
    15121 ccggtgcgca tcaacgaccc ccagtgcgtc gcgaagacct ttccagagta cttcgaggtg
    15181 ttctctgcca tcgttgcctg agggcgggga tataatgctc acgatgaaga aggtcgttac
    15241 gcgcacttca ctgaaggccc cgccacgaga ccgcgaatat tggctttcgc gcccgcccga
    15301 agagcgcctt gccgccgtcg agcagttgcg ccaggaggcc gccgcggggc gcccggatgc
    15361 tgaacaagga cttcagagag tttgtcgagt tactcggctc agccggggtt gagtacctaa
    15421 tcgtaggcgg ctacgcgctc gcggcgcatg gacatccgcg ctacaccggt gatctggaca
    15481 tctggatccg gtcgacgccc gcaaacgttg atcgattgct tcaggcgctg tccggattcg
    15541 gtttcggcag ccttggattg aaacgcgagg acttcctcca gcccagtgca gtcgtgcagc
    15601 tcggctaccc gccttcgcgg atcgatctgc tcacgggatt gagcggggtg gacttcgatg
    15661 cttgctatcc gaaccgcgcc gagatcacga tcgccggcgt gcgcctccca ttcattggcc
    15721 tggcggagtt ccgggccaac aagcgggcat cgggtcgagc caaggacctt gcggatctcg
    15781 agagtctcga cggcaaggac agttagaagc tcattcgatg acccggtcgg cgcgcagcag
    15841 cacggactgc gggatcgcga ggccgagagc cttcgcggtt ttcgcgttga tcgtcagctc
    15901 gaacttggtc ggcgcctcga tcggcaagtc gcccggtttg gcccccttga ggatcttgtc
    15961 gacgtagacg ctcaagcggc gcgcaagatc gttgttatcc gggctgtagg acatcaggca
    16021 gccctgctcg accagctcgg cctgcccgca cgaccccggc aacccgctcg ccaccacgag
    16081 ctggccgatc tcgtggcggc ggccgtatgc acgtgggctg cgctcgacca tgtatgcatc
    16141 aaccccttcc tgcttcagcc gcgagaacgc cggcgcgaaa tcccccggag cggattcgac
    16201 gaggaccatg gtcttgcccc gccgttccgc cgcggcccgc atcttgaccg cgtacggcct
    16261 ttcccactcc tcgcgcaacc cgacgaacgc gatgcgcctc gcgcgcggca cgagttcggc
    16321 gaacagctcg acgcgcttgg cttcgagatc ctcgtccacg tcgagggaaa gtcccgtgat
    16381 cgtgccaccc ggacgcccca tgctctgcgc gagacccgtg ctgatcaggt ggcctgcatt
    16441 caccagcccg acgatcggca gcgtcggtgc cgccttgtgc gcgggcggca cgagcagcga
    16501 cgaaggcagg aagacgactt ccatcttgag gcggacgaaa tcggccatga gcacttggag
    16561 gcgctcgggc tttccttcga gcgttcgcat ctccagaacg aggttcttgc cttggacgta
    16621 gccgagatcg cgcagcccat ggacgaatgc gcgcacaaaa gggttcgcag ggtcagggcc
    16681 cgagatttcg gcgagcggag aggtagtggc gacgaagccg acgcggtgaa tcttcttcgc
    16741 aggctgcgcg acgacgtgcg gcgcgatgag caccgcgccc gtgatctgca ggacagagcg
    16801 gcgtttcgtc ttgtggcccc ctttcgtgcg gcagaataac cgcgcgcatg tcggcacaca
    16861 tccccgtgat cgcgatcgat ggcccctcgg cgtcgggcaa gggcacgatc gccaagcgcg
    16921 tggccggggc gctcggcttc cactacctgg aaagcggggc cttgtaccgg ttggtcgccc
    16981 tggttgcctt gcggcagggc ctgcaggacg aagcggcgat cgccgacgcc gccgagcaca
    17041 tggacgtcgt gtttcaggaa gacgaaattc tccttgaaga ccaagatgtt agcgcacaaa
    17101 tccggcacga gacggtgggc agtcgggcgt ccgagatcgc gccgatgcgc aaggtgcggg
    17161 cggccctttt gacccgccag agggcgttcc ggcaaccccc cgggctggta gcggatgggc
    17221 gcgacatggg gaccatcgtg ttcccggatg cggcgctcaa aattttcttg acagcgggtc
    17281 cggaaatccg cgctcaacgc aggtataagc agttgataga aaaaggaatc gctgctaatc
    17341 tgggcgccct ttcgcgggac ctcgccgagc gcgacaagcg cgacgcgacc cgtgccgtgg
    17401 cgcccctggt tccggcgccc gattcgcaag tgctggattc gtcggcgctt tcgattgaag
    17461 agggggtcga ccgaattttg gagttatggg cgggcgggcc acgtggcggc gcccggtttg
    17521 caacttagcc cggagcccaa gtctccggtt tgaaaaggtt ttttcaattt acatggccag
    17581 tgtttccccg cagccgtcca aatcccccgc cagcgagtcg ttcgcggcgc tgttcgaaga
    17641 gagcctcaat cgcaaagaga tgcgcgtcgg cgaggtgatc accgccgagg tggtcagcat
    17701 cgacgacaac ttcgtagtgg tgaacgcggg gctgaagtcg gaaagcacga tcgcgatcga
    17761 agaattccgc accgacgccg ggcaggtcga cgtcaaggtc ggcgacttcg tcagcgtttc
    17821 catcgaagcg ctggaagacg gctacggctc gacgcgcctg tcgcgcgaca aggccaagcg
    17881 catggcggcc tggatcggcc tcgaaaaggc gctggagagc ggcgagaaga tcaagggcgt
    17941 cattaccggc aaggtgaagg gcggcctgac ggtcatggcc aacggcatcc gcgccttcct
    18001 gcccgggtcg ctggtcgacc tgcggccggt
//
LOCUS       DATBSZ010000012         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_616522, whole genome shotgun sequence.
ACCESSION   DATBSZ010000012 DATBSZ010000000
VERSION     DATBSZ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616522"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VIG70_00800"
     CDS             complement(<1..554)
                     /locus_tag="VIG70_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8249117.1"
                     /translation="MRASTRREYIAGPAGRIECAVDKPEGIARGVALIAHPHPLFGGT
                     LDNKVVQTLARALVELGYETWRPNFRGVGETEGVHDEGRGELEDLATIVNKLGPPTLV
                     LAGFSFGASLVARLAERVKPAWLVLVGLGITRLAAPPVPEGTLVIHGENDDTVPLAEV
                     LEWARPQELPVVLVPGADHFFHRKL"
     gene            complement(554..865)
                     /locus_tag="VIG70_00805"
     CDS             complement(554..865)
                     /locus_tag="VIG70_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEY8249118.1"
                     /translation="MSYYSKHVFFCCNQRDGGDRPCCNAKGASKMRDYAKGRIKALGL
                     AGEGKIRINQSGCLDRCEEGPCVVVYPDAVWYTYVDQSDIDEIIEEHLQNGRIVERLR
                     M"
     gene            complement(878..>1995)
                     /locus_tag="VIG70_00810"
     CDS             complement(878..>1995)
                     /locus_tag="VIG70_00810"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY8249119.1"
                     /translation="SRRPTTTASTELPAASGPTSGSALARILLLVYALLVVYATLYPL
                     AGWRDVGLSPLAYLTGPWPRHFTAFDLAVNVAGYVPLGLLAVVALYPRVRRLAAFAIA
                     VAGAAALSLSLEAGQSYLPARFPTNLDVLCNVGGAALGAALGQHFTPLIAGGPLSRWR
                     EAAFSRDTGTEFGLVLMALWLFIQLNPAALLFGAGDLRDYLAPLEGRARRPEFFVSTE
                     AFTAAANLVAVALILSVLAAPGRPVRAMVLGVVFAALVVKTAAFAILMRAENVLAWLT
                     HGAQLGLALGIAAALAAVALPRTARLITAAVLLMATTVLVNLSPPNPYLADSLKVWQQ
                     GHFLNFNGMTRLVSALWPFIALGYLMYLASRRREAVS"
BASE COUNT          354 a          657 c          703 g          281 t
ORIGIN      
        1 agcttgcggt gaaagaaatg atcggcgccg ggcaccagca ccaccggaag ctcctgcggc
       61 cgcgcccact ccagcacttc ggcgagcggc accgtgtcgt cgttctcgcc gtggatcacg
      121 agcgtgcctt cagggaccgg cggcgccgcg agccgcgtga ttcccaggcc gaccagcacc
      181 agccacgctg gcttcacccg ctcggcgagc cgcgcgacca gggatgcgcc gaaggaaaat
      241 ccggcaagaa caagcgtggg gggcccgagt ttgttcacga tggttgcaag atcttccagc
      301 tcgccgcggc cttcgtcgtg cacaccctcg gtttcaccga cgccgcgaaa gttcggtcgc
      361 caggtctcgt agccgagctc gacgagcgcg cgggctagcg tttgcaccac cttgttgtcg
      421 agcgtcccgc cgaagagggg gtgcggatgg gcgatgagcg cgacgccccg tgcgattccc
      481 tccggcttgt cgacggcgca ctcgatgcgt ccggccgggc cggcgatgta ttcgcgacgg
      541 gtgctcgccc gcattacatc ctgaggcgtt ctacgatgcg accgttctgc aggtgctcct
      601 cgatgatttc gtcgatgtcg gactggtcga cgtaggtgta ccagaccgcg tcggggtaca
      661 ccacgacgca ggggccctcc tcgcagcggt cgaggcaacc cgactggttg atgcggatct
      721 tgccttcgcc ggcgagaccg agcgccttga tgcgaccctt cgcgtagtcg cgcattttgg
      781 aagcgccctt ggcgttgcag caggggcgat cgccgccgtc gcgctgattg cagcagaaaa
      841 atacgtgctt cgagtagtag gacatgaacc ctgcattcta gctgaccgcc tctcgccgcc
      901 ttgaggcgag gtacatcaaa tagcccagcg cgatgaaggg ccataacgcg ctcacgaggc
      961 gcgtcatccc attgaagttg aggaagtgcc cctgctgcca gaccttcagc gaatcggcaa
     1021 ggtagggatt cggcggcgag aggttgacca gcaccgttgt cgccatcagc agcaccgcgg
     1081 cagtaataag gcgcgcagtg cgcggcaacg cgacggcggc gagcgcagcc gcgatgccga
     1141 gcgcgaggcc gagttgcgcg ccgtgcgtga gccaggcgag cacgttctcg gcgcgcatca
     1201 ggatcgcgaa cgcggccgtc ttcacgacca gcgcggcgaa caccacgccg aggaccatgg
     1261 cgcgcaccgg gcgtcccggc gccgccagca ccgagaggat gagcgcgacg gcgaccaggt
     1321 tcgccgcggc ggtgaacgct tcggtcgaga cgaagaactc cggccggcgc gcgcgcccct
     1381 ccagcggggc gaggtaatcc cggagatcgc cggcgccgaa aagcagcgcc gccgggttga
     1441 gctgaatgaa aagccatagc gccatcagca ccaggccgaa ttcggtcccg gtgtcgcggc
     1501 tgaacgcggc ttctcgccag cgcgacaacg gccctccggc gatgagcggc gtgaagtgct
     1561 gcccgagcgc ggcgccgagc gccgcgccgc caacgttgca cagcacgtcg aggttggtcg
     1621 ggaaccgcgc cgggaggtag ctctgcccgg cttcgagaga gagcgacagc gcggcagccc
     1681 ccgcgaccgc aatggcaaac gccgcgagac ggcgcaccct cgggtagagc gccacgacgg
     1741 cgagcaggcc caggggcacg tagccggcca cgttcaccgc caggtcgaat gcggtgaaat
     1801 ggcgcggcca gggcccggtc aggtaggcaa gcggcgagag gccgacgtcg cgccacccgg
     1861 caagcgggta aagcgtggcg taaacgacga gcagggcata cacaaggagc aggatgcgcg
     1921 cgagcgccga gccgctcgtc gggcctgacg ctgcggggag ctcagtgctt gccgtggtgg
     1981 tgggccgccg gctgg
//
LOCUS       DATBSZ010000013         9976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_753022, whole genome shotgun sequence.
ACCESSION   DATBSZ010000013 DATBSZ010000000
VERSION     DATBSZ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9976
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753022"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VIG70_00815"
     CDS             complement(<1..790)
                     /locus_tag="VIG70_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249120.1"
                     /translation="MSAYDDPPRRKLPVVRILLVVVAAGLIAAGIHFRARFETQPPQV
                     RLAPDSDVIGTAPLEISVTDAGAGLKSLSIMLGETSIAAERFASPVAEKKLSVALSKL
                     PGIKEGPATLRVVARDASLWGWFRGNQAVLERKLTLDLTPPTLEIVADDRYISFGGAG
                     ALVYKTSADAATSGVRVGKHFFPGFPGQIKDKPEHLLVFFAHPYDTPPGTRAVLVSTD
                     KAGNTRDMPLVYELKDVKYRKSTLTLSDGFLQNTVSALAREPAVR"
     gene            complement(795..1142)
                     /locus_tag="VIG70_00820"
     CDS             complement(795..1142)
                     /locus_tag="VIG70_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249121.1"
                     /translation="MATDSPETTRKALIDALLLKLPGVVGKKINKLDAYFVSDRMFAC
                     ISGEGLGLRLPVATVTELQFSRNDVVPFQPGNLSAGATREWVQINRPDAASYQQDLEL
                     IKTSLDFVKAGRR"
     gene            complement(1167..1242)
                     /locus_tag="VIG70_00825"
     tRNA            complement(1167..1242)
                     /locus_tag="VIG70_00825"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1206..1208),aa:Glu,seq:ttc)
     gene            complement(1276..1351)
                     /locus_tag="VIG70_00830"
     tRNA            complement(1276..1351)
                     /locus_tag="VIG70_00830"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1316..1318),aa:Ala,seq:ggc)
     gene            complement(1378..1779)
                     /locus_tag="VIG70_00835"
     CDS             complement(1378..1779)
                     /locus_tag="VIG70_00835"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEY8249122.1"
                     /translation="MRTFVLALAIAVSGSALADIEPGNWEMTVSTQVEGIPAGALAPI
                     VQTRCLTREDARDPSRLVGGAGCEFSDRRDTGSEMSFNVSCGGQLPMRGSGLVRYTSQ
                     SVDGSLELSAETGGQKIMTRSRVVGRRLGAC"
     gene            complement(1782..3149)
                     /gene="gltX"
                     /locus_tag="VIG70_00840"
     CDS             complement(1782..3149)
                     /gene="gltX"
                     /locus_tag="VIG70_00840"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY8249123.1"
                     /translation="MAGTALFRRGRHRLNVRTRFAPSPTGYLHIGGARTALFSWAYAR
                     RHGGVFVLRIEDTDLERSTQASVDAILDGLKWLGIDWDEGAFFQMKRLARYKEIADRL
                     VAAGHAYRDYMPKEELDALRAQQLARGEKPRYDRAIGQKYRGEGPAAIRFKTPQEGDV
                     GWNDLVKGPIAFPNAELDDLVLLRADGVPTYNFGVVVDDIDMKMTHVIRGDDHVNNTP
                     RQIHVYERLGTTIPDFGHVPMILGADGERLSKRHGATSVMDYRDAGFLPEALCNYLAR
                     LGWSHGDDEIFSMDQFKGWFDLEHVSASPARFDGEKLKWLNQQYIKSADAGRLASLVK
                     LPTDGPPVERVIDLVKDRANTLRQLADEAMLFYAKDVPEAAVPAEARDALRTLRARLE
                     KASWDRAAINAAIKETLAGAGLKMPQLAMPLRQVLTGRTQTPSIDAVLELLGRETVLR
                     RLERV"
     gene            complement(3107..4171)
                     /locus_tag="VIG70_00845"
     CDS             complement(3107..4171)
                     /locus_tag="VIG70_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8249124.1"
                     /translation="MIFRQLFDPQSSTYTYLLADPGSREALLIDPVFEQARRDAALID
                     ELGVKLAWTLETHVHADHVTGAWLLREKLGSRIALSQQSGAEGADRYLKPQDQIAFGK
                     RHVEARPTPGHTAGCITYVLDDRTMAFTGDALFIRGCGRTDFQGGDARTLYRSVREQI
                     FSLPDRCLVYPGHDYRGLTVTSVGEEKLYNPRLAESIGEGDFAGYMGNLGLPHPKQME
                     VAVPANLKCGKPEKTSSLDPDWAPLTYTFAGIWEVQPHWLEEHLRDVQIVDVREPDEF
                     NGPLGHVPGARLIPLGALAKNTENLSKATPLVMVCRSGARSAQATVLLGKAGFEKVAN
                     LSGGMLRWRAQRFSVEGGTD"
     gene            4374..4448
                     /locus_tag="VIG70_00850"
     tRNA            4374..4448
                     /locus_tag="VIG70_00850"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4406..4408,aa:Val,seq:cac)
     gene            4495..4698
                     /locus_tag="VIG70_00855"
     CDS             4495..4698
                     /locus_tag="VIG70_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249125.1"
                     /translation="MNAPLSIKQTLLHRAAKLLRGKDKLAVQLDVLEPLLDAWMDGTV
                     EMPDVKMPRLSAILSKAGDQTRG"
     gene            4792..5178
                     /locus_tag="VIG70_00860"
     CDS             4792..5178
                     /locus_tag="VIG70_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249126.1"
                     /translation="MKKDDADKPVPLHVAASVVHQQLSGSSRDWRDDTYDGILESTAL
                     ALSHVGHIYYVNTHGQALRIPDEELAVGMFENGGKRFRTRSGNVYESLSMLRGDMADA
                     LVILKSAQGAQRAGDSPAAEKPKKAT"
     gene            complement(5171..5485)
                     /locus_tag="VIG70_00865"
     CDS             complement(5171..5485)
                     /locus_tag="VIG70_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249127.1"
                     /translation="MSIKFQSLPFTEPLIEAAPDTTGVFALWQDGGVIYLGKASAGAA
                     TIRKALGEQFQGRRWNNVAGTRCSWEIAEDADKRYSELLREFEAAHHCAPLWNDPQRL
                     PT"
     gene            complement(5563..5862)
                     /locus_tag="VIG70_00870"
     CDS             complement(5563..5862)
                     /locus_tag="VIG70_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249128.1"
                     /translation="MPIASPRYAFTQTMVSGAPNDPGVYALWENDELIYYGHARGESV
                     TIQSSLKEKLAGRAGCTAGATHYGWEISANPPLREAELLREYQRAHRSLPRCNRG"
     gene            6018..6257
                     /locus_tag="VIG70_00875"
     CDS             6018..6257
                     /locus_tag="VIG70_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249129.1"
                     /translation="MPIKPNGALCAIKEQIVEDLASGLILQFECHEGRTRLVIAGRAL
                     PFGNREIIFDDQGKEAAAGTLLGEFRRPSWLKGVP"
     gene            complement(6254..6910)
                     /locus_tag="VIG70_00880"
     CDS             complement(6254..6910)
                     /locus_tag="VIG70_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249130.1"
                     /translation="MRFSIEPRDGCLHATVYERDTAEQTREFLLAVHAAAKTHGLSKV
                     LISIRQSRAIFKPEDYGLETGGYANALVAPGCPIALLGDTHEVSSANEYIEMVARQQG
                     LNVRAFRDEGTALRWLNGAGVPSRRYRFTRIVIAGAPDEAGVYALWDGEEVIYYGRAE
                     GENATIRSRLLDHYYGDSRRATHYSWEVCKDPRAREAELLGEHERAFGRLPRDNKKAA
                     "
     gene            complement(6911..7180)
                     /locus_tag="VIG70_00885"
     CDS             complement(6911..7180)
                     /locus_tag="VIG70_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249131.1"
                     /translation="MSAAEFDSLLETMAGLAAANIVLIETLHERGVVDKTHFAHAFAR
                     ALDTLAPEHRGGMIEHVLRNMRDRCMNLPAGEPSDAKEWLRQLLS"
     gene            complement(7177..>9976)
                     /locus_tag="VIG70_00890"
     CDS             complement(7177..>9976)
                     /locus_tag="VIG70_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8249132.1"
                     /translation="AQVTGVGGVWKDLTDNVNSMAANLTGQVRNIAEVTTAVARGDLS
                     KKITVDVKGEILELKNTVNTMVDQLNAFAGEVSRVAKEVGTEGKLGGQAQVKGVGGVW
                     KDLTDNVNSMAANLTSQVRSVASVVTAVAHGDLNRKLTVEAKGEVAALADTINSMIDT
                     LATFADQVTNVAREVGTEGKLGGQARVPGAAGTWRVLTENVNRLAANLSTQVRAIAEV
                     ATAVTKGDLTRSITVEALGEVASLKDNINEMIRNLKDTTRKNTEQDWLKTHLARFTRM
                     LQGHRDLVTVSRLILSELTPLVNAQQAAFYVADHRSGDSQLELLAGYAQRQGKTLPRK
                     IAFGEGLVGQCAFEKKRILLSQAPGDYIRIGSALGAGTPTSIIILPVLFEGQVKAVIE
                     LASFSKFSEIHQTFLDQLTESIGVVLHTIAANMQTEYLLHQSQSLTAELQSQQEELKK
                     TNDRLEQQALNLQKSESLLKSKQDELRSANDQLQEKARQLSEQMHQVEFKNREIEQAR
                     AALEEKAEQLSLSSRYKSEFLANMSHELRTPLNSLLILARLLADNVGGNLTPKQVEFA
                     RTIHSSGAELLALINDILDLAKIESGTVTLNIATERFDEVVDSAERTFRQMAADKGLE
                     FNVLVSPKLPPAIQTDGKRLQQILTNLLSNAFKFTSKGEVRLRIEPAASGWSMGHPVL
                     DRANPAIAFSVVDTGIGIPPNKQRLIFEAFQQADGSTSREYGGTGLGLSISRELARLL
                     GGEIRVASVQGQGSTFTLYLPLDYRPSGDAMGTMAGPQATQPAEDPLAPVSNEDAKAM
                     ALQANEALAGRKVLVVDDDIRNVFAMTSALETRSMRVLHAESGKEGIDTLKRERDVDL
                     ILMDVMMPGLDGLDTIRIIRSLDGYRGVPIVAVTAKAMVGDREKCIEAGANDYIAKPV
                     NVDVLLATAWRALQAAHA"
BASE COUNT         1534 a         3254 c         3372 g         1816 t
ORIGIN      
        1 gccgcaccgc gggctcgcgc gccagggccg acacggtgtt ctgcaggaag ccgtcggaca
       61 gcgtgagggt gctcttgcgg tacttcacgt ccttcagctc atagacgagc ggcatgtcgc
      121 gcgtgttgcc cgccttgtcg gtggacacca gcacggcccg ggttcccggc ggcgtgtcgt
      181 agggatgcgc gaagaacacg agcaggtgct cgggcttgtc cttgatctgg ccggggaagc
      241 cgggaaagaa atgcttgccg acccgcacgc cgctggtcgc ggcatcggcc gaggtcttgt
      301 agacgagggc accggcgccg ccgaagctga tatagcggtc gtcggcgacg atctcgagcg
      361 tgggcggcgt gaggtcgagc gtcagcttgc gctcgagcac cgcctggttg cccctgaacc
      421 agccccagag cgaggcatcg cgggcgacga cgcgcagcgt cgccggtcct tccttgattc
      481 ccggcagttt ggacagggcg acgctcagct ttttttccgc gaccggcgat gcgaaccgct
      541 cggcggcgat actggtttcc ccaagcatga tcgatagcga tttcaggccg gcgccggcat
      601 cggtcacgct gatctccagc ggcgcggtgc cgatcacatc ggagtccggc gcaagccgga
      661 cctgcggcgg ctgcgtctcg aagcgcgcac ggaagtggat tccggcggcg atcaggcccg
      721 cagcgacaac gacgaggagg atgcgaacga ccggaagctt gcggcgcgga ggatcgtcgt
      781 atgcgctcat gaccctacct tctgccggcc ttcacgaagt ccagcgaagt cttgatcagc
      841 tcgaggtcct gctggtagct ggccgcatcc ggtcggttga tctgcaccca ctcccgcgtg
      901 gctcccgcgc tgagattgcc gggctgaaac ggaacgacgt cgttcctcga gaactgcagc
      961 tcggtcacgg tcgcgacggg caggcgcagc cccaggcctt cgccgctgat gcaggcgaac
     1021 atccggtcgc tgacgaaata ggcgtcgagc ttgttgatct tcttgccgac caccccggga
     1081 agcttgagga gcagcgcgtc gatcaacgcc ttgcgggtcg tttcgggtga atcagttgcc
     1141 actgtaccgc tccacgctgg ttgggctggc gtccccacgg ggattcgaac cccggttacc
     1201 gccgtgaaag ggcgatgtcc taggcctcta gacgatgggg actgcgaggc gtcgtgcttc
     1261 ttctgctgcg tcggttggtg gaggtaagcg ggatcgaacc gctgacctct tgcatgccat
     1321 gcaagcgctc tcccagctga gctatacccc cgaccgaggg cgcggattat aacccggcta
     1381 acaggcgccg agccggcgtc cgacgactcg cgagcgtgtc atgattttct ggccgccggt
     1441 ctcggcggaa agctctagcg agccgtcgac ggattgcgac gtgtagcgca ccagaccact
     1501 accgcgcatg ggcagctgcc cgccgcagga gacgttgaag ctcatttccg agccggtgtc
     1561 gcgccggtcg gagaactcgc accccgctcc gccgaccagg cggctcggat cgcgcgcgtc
     1621 ctcgcgcgtc aggcagcgcg tctgcacgat cggcgccagg gccccggcgg ggatgccctc
     1681 gacctgggta ctcacggtca tctcccagtt gccgggctcg atgtcggcga gagcgctacc
     1741 gctgacggca atggcgagtg caaggacgaa ggtgcgcatg ttcagaccct ttccaggcgc
     1801 cgcagcacgg tctcgcggcc gagcagctcg agcaccgcgt cgatcgacgg cgtctgcgtg
     1861 cggccggtga gcacctggcg cagcggcatg gcgagttgcg gcatcttcag gcccgcgccg
     1921 gcgagcgttt ccttgattgc ggcgttgatg gcggcgcggt cccaggacgc tttctcgagc
     1981 ctggctcgca aagtccgcag cgcgtcgcgc gcctcggcgg gaacggcggc ctcgggaacg
     2041 tccttggcgt agaagagcat cgcctcgtcg gcgagctggc gcagcgtgtt cgcgcggtct
     2101 ttcaccaggt cgatgactcg ctccaccggc ggtccgtcgg tcggcagctt tacaagcgaa
     2161 gcaagtcgcc cggcgtccgc gctcttgatg tattgctggt tcagccactt cagcttctcg
     2221 ccgtcgaaac gcgccggcga ggcgctgacg tgctcgaggt cgaaccagcc cttgaactgg
     2281 tccatgctga agatctcgtc gtcgccgtgc gaccagccca ggcgcgcgag gtagttgcac
     2341 agcgcctccg gcaggaagcc ggcgtcgcga tagtccatca cgctggtcgc gccgtgccgc
     2401 ttcgacaggc gctcgccgtc ggcacccaga atcatgggca cgtgcccgaa gtcaggaatt
     2461 gttgttccta atctttcata aacatgaatc tgacgcggcg tattgttcac gtggtcgtcg
     2521 ccgcgaatga catgagtcat cttcatgtcg atgtcgtcga ccaccacgcc gaagttgtag
     2581 gtcggcacgc cgtcggcgcg cagcagcacg aggtcgtcga gctcggcgtt gggaaatgcg
     2641 atcggcccct tcacgaggtc gttccagccg acgtcgcctt cctgaggtgt cttgaagcga
     2701 atggcggccg gaccttcgcc gcggtacttc tgtccgatgg cgcggtcgta gcgcggcttc
     2761 tcgccgcggg cgagctgctg cgcccgcagg gcatcgagct cctccttcgg catgtagtcg
     2821 cggtacgcgt ggcccgcggc gacgagccga tcggcgattt ccttgtagcg cgcgaggcgc
     2881 ttcatctgga agaacgcgcc ctcgtcccag tcgatgccga gccacttgag gccgtcgagg
     2941 atcgcgtcga ccgaggcctg cgtcgagcgc tcgaggtcgg tgtcctcgat gcgcagcacg
     3001 aagacgccgc cgtggcgccg cgcgtaagcc cacgagaaga gcgcggtgcg cgcgccgccg
     3061 atgtgcaggt agcccgtggg actgggcgcg aaccgggtac ggacgttcag tcggtgccgc
     3121 cctcgacgga aaagcgctgt gcccgccacc gcagcatgcc gcccgagagg ttcgccacct
     3181 tctcgaagcc cgccttgccg agaagcaccg tggcctgcgc cgagcgcgcg ccggagcggc
     3241 ataccatgac cagcggcgtt gccttggaaa gattctccgt gttcttcgcg agcgctccga
     3301 ggggtatcaa gcgcgcgccg ggcacatggc cgagcgggcc gttgaactcg tcgggctcgc
     3361 gcacgtcgac gatctgcacg tcgcgcaggt gctcctccag ccagtgcggc tggacttccc
     3421 agatcccggc gaacgtgtag gtgagcgggg cccagtccgg atcgagggag ctcgtttttt
     3481 caggtttgcc gcacttcaag ttcgcgggaa cggcgacttc catctgcttc gggtgcggca
     3541 ggccgaggtt ccccatgtag ccggcgaagt cgccctcgcc gatcgattcc gcgaggcgcg
     3601 ggttgtagag cttctcctcg ccgacgctgg tgaccgtgag gccgcggtag tcgtgcccgg
     3661 gatagaccag gcagcggtcg ggcagcgaga aaatctgctc gcgcacgctg cggtagagcg
     3721 tgcgcgcgtc gccgccctgg aagtcggtgc ggccgcagcc gcggatgaag agcgcgtcgc
     3781 cggtgaacgc catcgtgcgg tcgtcgagca cgtaggtgat gcagccggcg gtgtggcctg
     3841 gagtgggccg ggcttcgacg tgccgcttgc cgaaggcaat ctggtcctga ggcttcaggt
     3901 acctgtctgc gccctccgcg ccgctttgct gagagagagc aatgcggctg cccagcttct
     3961 cgcgcagcag ccacgcgccg gtgacgtgat cggcgtgcac gtgggtctcg agcgtccagg
     4021 cgagcttcac gccgagctcg tcgatgagcg cggcgtcgcg ccgcgcctgc tcgaacaccg
     4081 ggtcgatcag cagcgcctcg cgcgaccccg ggtcggcaag caggtacgta taggtcgagg
     4141 actgcggatc gaacaactgg cgaaaaatca tcgcggcatc atataggtag ctccccggcg
     4201 gactgtccgc aaaaatgcgc gaaaatcgcg gggctcctgc atggataact ttactccagt
     4261 gcgccgctcg tcgtcggcgc tgcgatctcg aacctcagcg gggagattct ggttttcgtt
     4321 tctgccatga tcgccggtgg gctttttgct aaaatcgttt ctcctgccgg atcgggtgct
     4381 tagctcagcg gtagagcact tccctcacac ggaaggggtc gcaggttcaa aacctgcagc
     4441 acccaccaac aatcaaaggc ttagcgataa cctgagccgg tagtacgaaa aagtatgaat
     4501 gcccctctga gcatcaagca gacgctgtta caccgcgccg ccaagctttt acgcggcaag
     4561 gacaagctcg cggttcagtt ggacgtgctg gaaccgctgc ttgatgcttg gatggatggc
     4621 accgtcgaga tgccggacgt caaaatgccc cggctgtcgg ccattctcag caaggcaggc
     4681 gaccagacgc gggggtagcc tggcgagcgg cggcgacgcc gattttgctc tcagggcggc
     4741 gaatatccct tgaccgcctc gttgcggcga gcgcattgtg cttcaccccc catgaagaag
     4801 gacgacgcgg acaagccggt gccgctgcac gtcgcagcct cggtggtcca tcagcagctt
     4861 tcgggctcgt cgcgcgactg gagggacgac acctacgacg ggatcctcga gagcaccgca
     4921 ctcgcgctca gccacgtcgg gcacatctac tacgtgaaca cgcacggcca ggcgctgagg
     4981 atccccgacg aagagctggc cgtcggcatg ttcgagaacg gcggcaaacg gttccgcacg
     5041 cgctcgggga atgtctacga gtcgctgtcg atgctgcgcg gcgacatggc cgacgccctg
     5101 gtgatcctta agagcgcgca gggcgcgcag cgcgccggtg acagcccggc ggcggagaaa
     5161 ccgaagaaag ctacgtagga aggcgctgcg ggtcgttcca gagcggcgcg cagtggtgcg
     5221 cagcctcgaa ctcgcgcagc agctcgctgt agcgcttgtc cgcgtcctcc gcaatttccc
     5281 agctgcagcg cgtgccggca acgttgttcc accggcgccc ctggaactgc tcgcccagcg
     5341 ccttgcgaat cgtggccgcg cccgcggacg ccttgccgag atagatcacg ccgccgtcct
     5401 gccacagcgc gaacacgccg gtggtgtccg gcgcggcttc gatcagcggc tcggtgaacg
     5461 gcaggctttg gaacttgatc gacatttgga tctctccagc gattcccccg ggattccccg
     5521 cgcccatgtt acgcctcgcc gttgcagcgg ggcaagccgc tttcagccgc ggttgcaccg
     5581 cggcaaactt cgatgggcgc gctggtactc gcgcagcagc tcggcttcgc gcagcggcgg
     5641 gttcgccgag atttcccagc cgtagtgcgt cgcgcccgcg gtgcacccgg cgcggccggc
     5701 gagcttctcc ttgagcgaag actggatcgt cacgctctcg ccgcgcgcgt ggccgtagta
     5761 gatcagctcg tcgttctccc acagcgcgta tacgccgggg tcgttcggcg cgccggacac
     5821 catggtttgc gtgaaggcgt agcgagggct cgcgatcggc acttttttcc ctgccgaaaa
     5881 gcttacacca acgctgcgta gggtcgcccg ctgcatttcc ctagtagaaa tgcggtgtaa
     5941 cgcgtcatcg attcacgaca cgaacatggt acgcgtgttg cagggagaac ggatatcacc
     6001 catgcgatga ggcgtagatg ccgatcaagc cgaacggagc gttgtgcgcg atcaaggaac
     6061 agatcgtgga ggacctcgcg agcgggttga tcctccagtt cgaatgccat gaaggccgca
     6121 cgcgcctggt gatagcgggc cgcgcgctgc ctttcggcaa tcgcgagatc atcttcgacg
     6181 atcaggggaa agaagcggcc gccggcacgc tgctcggcga gttccgccgt ccctcgtggc
     6241 tgaaaggcgt cccctaggca gccttcttgt tgtcgcgcgg caggcggccg aacgcgcgct
     6301 cgtgctcccc gagcagctcg gcttcgcgcg cccggggatc cttgcagact tcccagctgt
     6361 agtgggtggc gcggcggctg tccccgtagt agtggtccag cagcctggag cggatggttg
     6421 cgttctcgcc ttccgcgcgg ccgtagtaga tcacttcttc cccgtcccat agcgcgtaga
     6481 cgccggcctc gtcgggcgcg cccgcgatca cgatgcgcgt gaagcggtag cggcggctcg
     6541 gcacgccggc gccgttcagc cagcgcagcg ccgtgccctc gtcgcggaag gcgcgcacgt
     6601 tcaggccctg ctggcgcgcc accatctcga tgtactcgtt cgccgagctg acctcgtggg
     6661 tgtcgccgag cagggcgatc gggcaacccg gcgcgaccag ggcgttggcg tagccgccgg
     6721 tctcgaggcc gtagtcttcg ggcttgaaga tggcgcgcga ctgccggatg gaaataagga
     6781 ctttcgacag gccgtgcgtc ttggcggcgg cgtgcacggc gagcaggaat tcccgcgtct
     6841 gctcggcggt gtcgcgctca tagaccgtcg cgtgcaggca gccgtcgcgc ggctcgatcg
     6901 agaagcgcat ctagctcaac agctggcgca gccactcctt cgcgtcggag ggctcgcccg
     6961 cgggaagatt catgcagcgg tcgcgcatgt tgcgcagcac gtgctcgatc atgccgccgc
     7021 ggtgctcggg ggcgagcgtg tcgagcgcgc gtgcgaaggc gtgcgcgaag tgcgtcttgt
     7081 ccaccacgcc gcgctcgtgc agcgtctcga tcaggacgat gttcgccgcc gcgagaccgg
     7141 ccatggtctc cagcagcgaa tcgaattccg cagcgctcac gcgtgcgccg cctgcagcgc
     7201 tctccatgcg gtggcgagca gcacgtcgac gttgaccggc ttggcgatgt agtcgttggc
     7261 gccggcctcg atgcacttct cgcggtcgcc gaccatggcc ttcgcggtca cggcgacgat
     7321 cggcacgccg cggtagccgt ccaggctgcg gatgatgcgg atggtgtcga ggccgtcgag
     7381 ccccggcatc atcacgtcca tcaggatgag atcgacgtcg cgctcgcgct tgagcgtgtc
     7441 gatgccctcc ttgccgctct cggcgtgcag cacccgcatg ctgcgggtct cgagcgcgct
     7501 ggtcatggcg aacacgttgc ggatgtcgtc gtcgaccacc agcaccttgc ggccggcgag
     7561 cgcctcgttg gcctgcagcg ccatcgcttt cgcgtcctcg ttcgagaccg gcgcgagcgg
     7621 gtcttccgcg ggctgggttg cctgcgggcc ggccatcgtg cccatcgcgt ccccggaagg
     7681 ccggtagtcg agcggcaggt agagcgtgaa cgtgctgccc tggccctgca cgctcgccac
     7741 ccggatctcg ccgccgagca ggcgcgcgag ctcgcggctg atcgacaggc ccaggcccgt
     7801 gccgccgtac tcgcggctgg tggaaccgtc ggcctgctgg aaggcttcga agatcaggcg
     7861 ctgcttgttg ggcgggatgc cgatgccggt gtcgaccacc gagaaggcga tcgccggatt
     7921 ggcacggtcg agcaccgggt ggcccatgct ccagcccgag gccgcgggct cgatgcgcag
     7981 gcgcacctcg cccttgctgg tgaacttgaa cgcattcgaa agcagattcg tgaggatctg
     8041 ctgcaggcgc ttgccgtcgg tctgaatcgc cggcggcagc ttcgggctca ccagcacgtt
     8101 gaactcgagg cccttgtcgg ccgccatctg gcggaaggtg cgctcggccg agtcgaccac
     8161 ctcgtcgaag cgctcggtcg cgatgttgag cgtgaccgta cccgactcga tcttcgccag
     8221 gtccaggatg tcgttgatca gcgccagcag ctcggcgccc gacgaatgga tggtgcgcgc
     8281 gaactcgacc tgcttcggcg tgagattgcc gccgacgttg tcggcgagca gccgcgcgag
     8341 gatcaacagg ctgttgagcg gcgtgcgcag ctcgtgggac atgttcgcca gaaattccga
     8401 cttgtatcgg gaagacaagg acaactgctc ggctttctct tccagcgccg cgcgcgcctg
     8461 ctcgatctcg cggttcttga actcgacctg gtgcatctgc tcggagagct ggcgcgcctt
     8521 ctcctgcagc tggtcgttcg ccgagcgcag ctcgtcctgc ttggatttca ggagcgactc
     8581 ggacttctgc aggttgagcg cctgctgctc caggcggtcg ttggtcttct tgagctcttc
     8641 ctgctggctc tgcagctcgg cggtcagcga ctgcgactgg tgcagcaggt attcggtctg
     8701 catgttcgcc gcaatagtat ggagcacgac gccgatcgat tcggtgagct ggtcgaggaa
     8761 ggtctggtgg atctcgctga acttggagaa cgaggcgagc tcgatcaccg ctttcacctg
     8821 cccctcgaac agcaccggca ggatgatgat gctggtgggc gtgccggcgc cgagcgccga
     8881 gccgatgcgg atgtagtcgc ccggcgcctg cgagaggagg atgcgcttct tctcgaacgc
     8941 gcactgcccg accaggcctt cgccgaacgc gatcttgcgc ggcagcgtct tgccctggcg
     9001 ctgcgcgtag ccggcgagca gctcgagctg cgagtctccg ctgcggtggt cggcgacgta
     9061 gaacgcggct tgttgcgcgt taaccagggg ggttagctcg ctcaagatca atcgggaaac
     9121 ggtgacgagg tcccgatgtc cctggagcat gcgcgtgaac cgcgcgaggt gggtcttcag
     9181 ccagtcctgc tcggtgttct tgcgcgtggt gtccttcagg ttgcggatca tctcgttgat
     9241 gttgtccttc aacgaggcca cttcaccgag cgcttcgacc gtgatcgagc gcgtcagatc
     9301 gcccttggtc accgcggtcg ccacttcggc gatggcacgc acctgcgtcg agaggttcgc
     9361 cgcgaggcgg ttcacgttct cggtgagcac gcgccaggtg ccggcggcgc ccggcacgcg
     9421 cgcctgcccg cccagcttgc cttcggtgcc tacctcgcgc gccacgttgg tcacctggtc
     9481 cgcgaacgtc gccagcgtgt cgatcatgct gttgatggtg tcggcgagcg ccgcgacctc
     9541 gcccttcgcc tcgaccgtca gcttgcggtt gaggtcgccg tgcgcgaccg ccgtcaccac
     9601 gctcgcaacg ctgcgcacct ggctggtgag gttggcggcc atcgagttca cgttgtcggt
     9661 gaggtccttc cacacgccgc cgacgccctt cacctgcgcc tggccgccga gcttgccttc
     9721 ggttcccact tccttggcga cccgggagac ttccccggca aaggcgttga gctgatccac
     9781 catcgtgttc acggtgttct tcagctccag gatctcgccc ttcacgtcca ccgtgatctt
     9841 cttggagaga tcgccgcgcg cgaccgcggt ggtcacctcg gcgatgttcc tcacctggcc
     9901 ggtgaggttg gccgccatcg agttcacgtt gtcggtgagg tccttccaca cgccgccgac
     9961 gccggtcacc tgcgcc
//
LOCUS       DATBSZ010000014         1741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1641231, whole genome shotgun sequence.
ACCESSION   DATBSZ010000014 DATBSZ010000000
VERSION     DATBSZ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641231"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VIG70_00895"
     CDS             complement(<1..357)
                     /locus_tag="VIG70_00895"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEY8249133.1"
                     /translation="MDRRKFLLAGAAAPLWMYPFHAHAQPKTMKDIEALQKNWKTFLA
                     AGAQVPSATEALKLSKDEWKKRLPDSMAYRVLREEGTERAGSSPLNDEKRAGIFACAG
                     CGLPVFTSEMKYESGTG"
     gene            445..1416
                     /locus_tag="VIG70_00900"
     CDS             445..1416
                     /locus_tag="VIG70_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249134.1"
                     /translation="MTRIPRILSAAILALVWIGAGAQQFPTKPIHLVSPYAAGGAMDL
                     TARSLAAVMSETIGQSVVVENRTGGNGLIGTDYVSKAAPDGYTLLMYADANAIAPAIY
                     KKLNHDPVAGFAPITNTILGTHLIVGHPSVPANSLKELIELARKPGSNLTFGSPGTGT
                     PQHLAGELFKQQAGGLDITHVPYRGGGQLIVDLVAGQVPLGLIGLPPTLPHIKAGKLK
                     VFAVTARKRSPSLPDVPTVEEAGLAGYETIQWFGPVAPAGTPKPVIDKLFEHFVKALQ
                     HPSVAERLTKAGLEVAPSASPEAYGAFIRAEVARWPAIVKAAGVEPQ"
     gene            1420..>1741
                     /locus_tag="VIG70_00905"
     CDS             1420..>1741
                     /locus_tag="VIG70_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY8249135.1"
                     /translation="MSRGLSILFITSDQQRGDCYGFEGRRVKTPHLDELAAAGTRFSA
                     CITPNVVCQPSRASILTGLLPRTHGVADNGIDLDEDVGADGFGGTFARAGWRTAFIGK
                     AHFST"
BASE COUNT          273 a          634 c          558 g          276 t
ORIGIN      
        1 gccggtgccg gattcgtact tcatctcgga ggtgaagacc ggcagcccgc agccggcgca
       61 agcgaagatg ccggcgcgct tctcgtcgtt cagcgggctg ctgccggcac gctcggtccc
      121 ctcctcgcgc agcacgcggt acgccatgga atcgggcagg cgcttcttcc actcgtcctt
      181 cgagagcttg agcgcttccg tcgctgacgg aacctgcgct cccgcggcca ggaaggtctt
      241 ccagttcttc tgcagcgctt cgatgtcttt catcgtcttg ggctgtgcgt gggcgtggaa
      301 gggatacatc catagcggcg ccgccgcccc tgcgagcaag aatttccgtc ggtccatggg
      361 ctcctcatcg gttctgcctt ggtcgagcta acgctcaaaa cctaacacga attccgctac
      421 gatgcgggca gggaggaacc tcgagtgaca cgcatcccac gcattctttc ggccgccatc
      481 ctggccctcg tctggatcgg cgccggcgcg cagcagttcc cgacgaagcc gatccacctc
      541 gtctcgccct acgctgcggg cggcgccatg gacctgaccg cgcgctcgct cgccgcggtc
      601 atgtcggaga ccatcggcca gtcggtagtg gtggagaacc gcactggcgg caacggcctg
      661 atcggcaccg actacgtgtc gaaggccgcg cccgacggct acacgctcct gatgtatgcc
      721 gacgccaacg cgatcgcgcc tgcgatctac aagaagctga accacgatcc ggtcgcgggc
      781 ttcgcgccga tcaccaatac catcctcggc acccacctca tcgtcggcca tccctcggtg
      841 ccggcgaaca gcctgaagga gctgatcgag ctcgcgcgca agcccggctc gaacctcacc
      901 ttcggctcgc cgggaaccgg cacgccgcaa cacctcgcgg gcgagctctt caagcagcag
      961 gccggagggc tcgatatcac ccacgtgccc taccgcggcg gcggccagct catcgtcgac
     1021 ctcgtcgccg gccaggtgcc gctcggcctg atcgggctgc cgccaacgct gccgcacatc
     1081 aaggcgggga agctcaaggt gttcgccgtg accgcgcgca agcgctcgcc gtcgctgccc
     1141 gacgtgccga cggtcgagga agccgggctc gccggctacg agacgatcca gtggttcggg
     1201 ccggtcgcgc ccgccggcac gccgaagccg gtcatcgaca agttgttcga gcacttcgtg
     1261 aaggcgctgc agcatccgag cgtggccgag cggctgacga aagcgggcct ggaggtggcg
     1321 ccgagcgcct cgcccgaggc ctatggcgcg ttcatccgcg ccgaagtcgc gcgctggccg
     1381 gcgatcgtca aggccgcggg cgtggagccc cagtaagcga tgagccgcgg gctcagcatc
     1441 ctcttcatca cctcggacca gcagcgcggc gactgctacg gcttcgaggg gcggcgcgtg
     1501 aagacgccgc atctcgacga actcgccgcc gccggcacgc gcttctccgc ctgcatcacg
     1561 cccaacgtgg tctgccagcc ctcgcgcgcg tcgatcctca ccgggctgct gccgcgcacg
     1621 cacggcgtcg ccgacaacgg catcgacctc gacgaagacg tcggcgccga cggcttcggc
     1681 ggcacattcg cgcgcgccgg ctggcgcacc gcgttcatcg gcaaggcgca tttctcgacg
     1741 t
//
LOCUS       DATBSZ010000015         3615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2051104, whole genome shotgun sequence.
ACCESSION   DATBSZ010000015 DATBSZ010000000
VERSION     DATBSZ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2051104"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VIG70_00910"
     CDS             <1..1246
                     /locus_tag="VIG70_00910"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY8249136.1"
                     /translation="GRLLLRGLQFAIVDEADSVLVDEARTPLILSAGSERTAGEKMHR
                     DALEIAGALGESDYRIGEEGIEITDTGQDALARVAKDLGGIWKGPRRREQLVRQALTA
                     LHVFQRDKHYLVREGKVQIIDEYTGRLMPDRSWERGLHQLIEMKEEVEVTAERETLAR
                     ISYQRFFRRYLHLSGMTGTAAEIAGELRAVYRLRVARIPTNRPSRRARQPARYFGSAQ
                     AKWQAVVDTIAACIARGQPVLVGTRSVGASELLAKLLDEKGVPYRLLNARQDRDEAEI
                     VARAGERGCVTVATNMAGRGTDIRLAEGVRELGGLHVVATELHDAARIDRQLFGRCGR
                     QGDPGSYEVIVAMEDDLAKTFAKLASAPLTGLERIPSALGGAVFRLAQWRAERAHSRA
                     RRDLLDLDDYLGDILAFSGRGE"
     gene            complement(1262..1702)
                     /locus_tag="VIG70_00915"
     CDS             complement(1262..1702)
                     /locus_tag="VIG70_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249137.1"
                     /translation="MQGPVRLKVLAAFLLSLAAAAFAAEAELSAEVPAEKWRALRLKG
                     LTQGASLRVRVETSGPIRVILAREDEVQRFPRGLKATFTGSAERRLSFAVSIPAAGTY
                     YVILDNRKGDALREVKLFVEALPARKPRPKPAPKPKSEPGLNAT"
     gene            complement(1681..2478)
                     /locus_tag="VIG70_00920"
     CDS             complement(1681..2478)
                     /locus_tag="VIG70_00920"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8249138.1"
                     /translation="MRLPAALFACLLATSASAAGEYNCLIEAYESVDVRSPVEALIES
                     ILVRRGDVVKKGQVLVRLESAAERAGLDLAKARATMQGEIKAAEARVDLARRKHARAE
                     ELFKQNFVSSTARDEAEAEFKLASEQLRQAQEARQLAELEARRSSELVAMRTLRSPFA
                     GVVVEKLQSPGEMATTNINQPILKLARIDPLHVEVVLPVSEYGKIRNGMKGLVVPEKP
                     IEGRYEAIVKVVDRTVDAASGTFGARLELPNPKAAIPAGVKCRVRFG"
     gene            complement(2511..3464)
                     /locus_tag="VIG70_00925"
     CDS             complement(2511..3464)
                     /locus_tag="VIG70_00925"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEY8249139.1"
                     /translation="MSAAHQPVAMVAVGGNALIRDAQHSSLSDQYDAVCATAAHVVDM
                     IAAGWNVVLTHGNGPQVGFILRRSELSIAEVPPVPMDYAGADIQGAVGYMFVKALRNV
                     FKRRRIKRAAVAVVTQTVVDRNDPAFANPLKPIGSHMEEARAKQLAAKYRWTVREDAG
                     RGWRRVVPSPQPKAIIETGVIRALASQGYVVVACGGGGIPVIEDENGDLQGVEAVIDK
                     DLASSLLARSLQADALVLPTGVEKVALDFNKPTQRWLDRLTLAEARKHHDNDQFDKGS
                     MGPKVAALIDFVGGGGRFGLITDPPNLTRALRGETGTRVVA"
BASE COUNT          506 a         1292 c         1258 g          559 t
ORIGIN      
        1 cggccggttg ctgctgcgcg gcctgcagtt cgccatcgtc gacgaggccg acagcgtgct
       61 ggtggacgag gcacgcacgc cgctcatcct ctccgccggc tcggagcgca ccgcgggcga
      121 gaagatgcac cgcgacgcgc tggagatcgc gggcgcgctg ggcgagtccg actaccgcat
      181 cggcgaggaa ggcatcgaga tcaccgacac cggccaggac gccctcgcgc gcgtcgccaa
      241 ggacctgggc gggatctgga aagggccgcg ccgccgcgag cagctggtgc gccaggcgct
      301 cactgcgctg catgtcttcc agcgcgacaa gcactacctc gtgcgcgagg gcaaggtgca
      361 gatcatcgac gagtacaccg gccggctgat gcccgaccgc tcctgggagc gcgggctgca
      421 ccagctgatc gagatgaagg aagaggtcga agtgactgcc gagcgcgaga cgctggcgcg
      481 catcagctac cagcgcttct tccggcgcta ccttcacctt agcggcatga ccggcaccgc
      541 ggccgagatc gccggcgagc tgcgcgcggt ctaccggctg cgcgtcgcgc gcattccgac
      601 caaccggccg agccgccgcg cgcgccagcc ggcgcgctac ttcggctcgg cgcaggcgaa
      661 gtggcaggcg gtggtcgaca ccatcgccgc ctgcatcgcg cgcggccagc ccgtcctggt
      721 cggcacgcgc tcggtcggcg catcggagtt gctagccaaa cttttggacg aaaagggtgt
      781 gccgtaccgg ttgctcaacg cgcgccagga ccgcgacgaa gccgagatcg tggcacgcgc
      841 cggcgagcgc ggctgcgtca ccgtggcgac caacatggcg gggaggggaa ccgacatccg
      901 gctcgcggag ggcgtgcgcg agctcggcgg cctgcacgtc gtcgccaccg agctgcacga
      961 cgccgcacgc atcgaccggc agctcttcgg gcgctgcgga cgccagggcg atccgggaag
     1021 ctacgaagtc atcgtcgcca tggaggacga cttggcgaag actttcgcca agcttgcatc
     1081 cgcgcccctc accgggctgg agcgcatccc gtccgcgctg ggaggggcgg tgttccggct
     1141 cgcgcagtgg cgcgccgagc gcgcgcactc gcgcgcgcgg cgcgacctgc tcgacctcga
     1201 cgactatctg ggagacatcc tcgccttctc ggggcgcggc gagtagctcg ggtttcgcta
     1261 gtcaggttgc attgagcccg ggctcggact tcggcttcgg cgcgggcttc ggccgcggct
     1321 tgcgcgccgg cagcgcctcg acaaagagct tcacctcgcg cagcgcatcg cccttgcggt
     1381 tgtcgaggat cacgtagtag gtgcccgcgg cgggaatgct gaccgcgaag ctcaggcggc
     1441 gctcggcgct gccggtgaag gtggccttga gccctctcgg aaagcgctga acctcgtcct
     1501 cgcgcgcgag gatcacgcgg atcggccccg aggtttccac ccgcacgcgg agcgaggctc
     1561 cctgcgtcag ccccttcagg cgcagcgcgc gccatttctc ggccggcacc tcggcgctaa
     1621 gctcggcttc ggcggcgaaa gccgccgccg cgagcgacag caggaacgcc gcgagaactt
     1681 tcagccgaac cggaccctgc acttgacccc ggcgggaatg gcggctttcg ggttgggcag
     1741 ctcgaggcgc gcgccgaaag tgccgctcgc cgcgtccacc gtgcggtcga ccaccttgac
     1801 gatcgcttcg tagcgcccct cgatcggctt ctccggcacc accaagcctt tcatgccgtt
     1861 cctgatcttg ccgtactcgc tgaccggcag caccacctcc acgtgcagcg ggtcgatgcg
     1921 cgcgagcttg aggatcggct ggttgatatt ggtggtcgcc atctcgcccg gcgactgcag
     1981 cttctcgacc accacgccgg cgaacgggct gcgcagcgtg cgcatcgcga ccagctccga
     2041 gctgcgccgc gcctcgagct cggcgagctg gcgcgcttcc tgcgcctggc gcagctgctc
     2101 gctcgccagc ttgaactcgg cctcggcctc gtcgcgcgcc gtcgaggaga cgaagttctg
     2161 cttgaacagc tcctcggcgc gggcgtgctt gcggcgcgcg aggtccacgc gcgcctcggc
     2221 ggccttgatc tcgccctgca tggtggcgcg cgccttcgcc aggtcgagcc ccgcgcgctc
     2281 ggccgccgac tccaggcgca ccagcacctg gccctttttc accacgtcgc cgcggcgtac
     2341 caggatggat tcgatcagcg cttcgaccgg gctgcgcacg tcgacgctct cgtaggcctc
     2401 gatcaagcag ttgtattccc ccgcggcgct cgcgctggtg gcgagcaggc aggcgaaaag
     2461 ggcggcgggc aatctcatgc gcccgcgtag cttatccgct ttttcttcag ctaagcgacg
     2521 acgcgcgtcc cggtctcgcc gcgcagcgcg cgcgtgaggt tgggcggatc ggtgatcagg
     2581 ccgaagcggc cgccgccgcc gacgaagtcg atcagcgccg ccaccttggg gcccatgctg
     2641 cccttgtcga actggtcgtt gtcatggtgc ttgcgcgcct cggcgagcgt caggcggtcg
     2701 agccagcgct gcgtcggctt gttgaagtcg agcgcgactt tttccacgcc ggtgggcagc
     2761 accagcgcgt cggcctgcag gctgcgcgcg agcaggctcg aggcgaggtc cttgtcgatc
     2821 accgcctcga cgccctggag gtcgccgttc tcgtcctcga tcaccgggat gccgccgccg
     2881 ccgcaggcga cgaccacgta gccctggctc gcgagcgcgc ggatcacgcc ggtctcgatg
     2941 atcgccttcg gctgcggcga ggggaccacg cgccgccagc cgcggcccgc gtcctcgcgc
     3001 acggtccagc ggtatttcgc ggcgagctgc ttcgcccgcg cttcttccat gtgcgagccg
     3061 atcggcttga gcgggttcgc gaacgccggg tcgttgcggt cgaccacggt ctgcgtgacc
     3121 accgcgaccg ccgcgcgctt gatgcgccgg cgcttgaaca cgttgcgcag cgccttgacg
     3181 aacatgtagc cgaccgcgcc ctggatgtcg gcgccggcgt agtccatcgg caccggcggc
     3241 acttcggcga tcgacagctc ggagcggcgc aggatgaagc cgacctgcgg gccgttgccg
     3301 tgggtgagca cgacgttcca gcccgcggcg atcatgtcga cgacgtgcgc ggccgtggcg
     3361 cagacggcat cgtactggtc gctgagcgac gaatgctgcg cgtcacggat gagcgcgttg
     3421 cctcctaccg ccaccatcgc aacgggctgg tgggcggcgc tcaccgtgtc tccgacccga
     3481 cgtcgtgcgc gagcgccttc accgccgcgg cgaagcatgc catcggcgcg gtggtgactc
     3541 cggcgccgat ctgcccgacc cccgcttgcc gatgggcgat gccgctgttg atcaccggca
     3601 gcacgccgca atcga
//
LOCUS       DATBSZ010000016         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1436447, whole genome shotgun sequence.
ACCESSION   DATBSZ010000016 DATBSZ010000000
VERSION     DATBSZ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436447"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VIG70_00930"
     CDS             <1..339
                     /locus_tag="VIG70_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8249140.1"
                     /translation="PEPKHGIVAAIVMANLVRQVGRKAAFELVALGEPVDAQRALQLG
                     MVNRVVPLSELMREAIALAEKLAAVKRPAMAETKRLFHEVADLPLAPALERGRDTNKR
                     MRNFQQLGKK"
     gene            336..1514
                     /locus_tag="VIG70_00935"
     CDS             336..1514
                     /locus_tag="VIG70_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY8249141.1"
                     /translation="MKPLDQLTILDLSRVLACPFASMILAELGARVIKVEQPGSGDET
                     RGFEPQLARDSAYYFACNRSKESITVNLRSDEGRKLIRSLAQKVDVVLENFPVGTLGR
                     YGLDYAAMSALNPSLVFVSCTGFGQTGPYAEKKGYDTVFQAMGGIMSLTGEKGGAPVK
                     PGLPVADLTSGLWIAIAILAALAGRARTGKGCYVDFSMLDGQVSLLTLAAARYFALNE
                     IPPRLGTEHPGRVPSATFRCKDGKYAHITASDQHWAPLCRVLGLETWGAAFSENSIRV
                     EKRDEIMPVLSEKIGKLDRANLLAILDEADVPVGPVNDVAEILGDPHVRARRLVGSFD
                     YPEVGEFKALAVPYKFLGWDNPEIGRPPALGEHTESVLSDMLGLSSKRIAELKAAKAI
                     "
     gene            1511..>1941
                     /locus_tag="VIG70_00940"
     CDS             1511..>1941
                     /locus_tag="VIG70_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY8249142.1"
                     /translation="MTVEFSVADGVATIALNRPDVRNALNLEMCDELLRVSKELVEKK
                     VRVVFVRGNGPVFCGGADLNERKGKSEDWIRARRMRAFAAYGALESLPMPCVALVHGA
                     AIGSGVEIAAACDFIVATPDASFRTPEAQRGTVGATQRLPR"
BASE COUNT          332 a          628 c          664 g          317 t
ORIGIN      
        1 cccgagccga agcacggcat cgtcgcggcg atcgtgatgg cgaacctggt gcgccaggtc
       61 gggcgcaagg cggcgttcga gctggtcgcg ctcggcgagc cggtcgatgc gcagcgggcc
      121 ttgcagctcg ggatggtgaa cagggtcgtt ccgctttcag aactcatgcg tgaagcgatt
      181 gcgctggcgg agaaactcgc ggcggtgaag cgtcccgcca tggcggagac caagcgcctg
      241 tttcacgaag tggcggacct gccgctcgcg ccggcgctgg agcgcggccg cgataccaac
      301 aagcgcatgc gcaattttca gcagctcggc aagaagtgaa acctctcgat cagcttacga
      361 tcctcgatct gtcgcgcgta ctcgcgtgcc cgttcgcgtc gatgatcctc gccgaactcg
      421 gcgcccgtgt gatcaaggtc gagcagcccg gcagcggcga cgagacccgc ggcttcgagc
      481 cccagctcgc cagggacagc gcgtactact tcgcctgcaa ccgctcgaaa gaatccatta
      541 ccgtgaacct tcgctcggac gaaggcagga agctgatccg cagcctcgcg cagaaggtgg
      601 acgtggtgct ggagaacttc ccggtcggca cgctggggcg gtatgggctg gattacgccg
      661 cgatgtcagc actcaatccc agccttgttt ttgtttcctg cacgggattc gggcaaaccg
      721 gcccgtacgc tgaaaagaag ggctacgaca ccgtgttcca ggcgatgggc gggatcatga
      781 gcctgaccgg cgagaagggc ggcgcgccgg tgaagccggg cctgccggtc gccgatctca
      841 cctccggcct ctggatcgcg atcgcgatcc tcgcggcgct cgccgggcgc gcgcgtaccg
      901 gcaagggctg ctacgtcgat ttctcgatgc tcgacggcca ggtgtcgctc ctcaccctgg
      961 cggcggccag gtactttgcg ctgaacgaga tcccgccgcg gctcggcacc gagcatcccg
     1021 gccgcgtgcc gtcggcgact ttccgctgca aggacggcaa gtacgcgcat atcaccgcga
     1081 gcgaccagca ttgggcgccg ctgtgccgcg ttctcggcct ggagacctgg ggcgcggctt
     1141 tttcggagaa ctccatccgg gtggagaaac gcgacgagat catgcccgtg ctcagcgaga
     1201 agatcgggaa actggatagg gcaaacctgc tcgccatcct cgacgaagcc gacgtgccgg
     1261 tcggcccggt gaacgatgtt gccgagatcc tgggcgaccc gcacgtgcgg gcgcggcgcc
     1321 tcgtcggcag cttcgactac cccgaggtgg gcgaattcaa ggcgctcgcc gttccgtaca
     1381 agtttctcgg ctgggacaac cccgagatcg gccgcccgcc ggcgctgggc gagcacaccg
     1441 agagcgtgct atccgacatg ctgggcctgt catcgaaaag gatcgctgag ctgaaggcgg
     1501 cgaaagcgat atgaccgtcg agttctcggt cgcggacgga gtggccacca ttgcgctgaa
     1561 tcgccccgat gtgcgcaacg cgctcaacct cgaaatgtgc gacgagctcc tgcgggtttc
     1621 aaaagagctg gtcgaaaaga aggtgcgtgt ggtcttcgtg cgggggaacg gcccggtgtt
     1681 ctgcggcgga gccgatctca acgagcgcaa aggcaagagc gaggactgga tccgcgcgcg
     1741 acgcatgcgc gcgttcgcgg cctatggggc gctcgagtct ctgccgatgc cctgcgtcgc
     1801 gctcgtgcac ggcgcagcga tcgggtccgg ggtcgagatc gcagccgcgt gcgatttcat
     1861 cgtcgccacg cccgacgcct cgttccgcac ccccgaagcg cagcgcggca cggtcggcgc
     1921 cacgcagcgc ctgccgcgca t
//
LOCUS       DATBSZ010000017        21367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2392963, whole genome shotgun sequence.
ACCESSION   DATBSZ010000017 DATBSZ010000000
VERSION     DATBSZ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21367
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392963"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /gene="prpE"
                     /locus_tag="VIG70_00945"
                     /gene_synonym="yahU"
     CDS             <1..741
                     /gene="prpE"
                     /locus_tag="VIG70_00945"
                     /gene_synonym="yahU"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928986.1"
                     /note="catalyzes the formation of propionyl-CoA using
                     propionate as a substrate; PrpE from Ralstonia
                     solanacearum can produce acetyl-, propionyl-, butyryl- and
                     acrylyl-coenzyme A, and Salmonella enterica produces
                     propionyl- and butyryl-coenzyme A; not expressed in
                     Escherichia coli when grown on propionate/minimal media;
                     ATP-dependent; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HEY8249143.1"
                     /translation="EALGRPVIDHYWQTETGWPLLSAHPGVEKTPLKMGSPSFPCYDY
                     DVRLLHEGSGKEVKDGEKGVVTVVPPLPPGCMSTVWGDDERFVNTYFNDFKGQQVYST
                     FDWGIRDQDGYYFILGRTDDVINVAGHRLGTREIEEAVSMHPNIAECAVVGVADALKG
                     QMPLAFAVLKDSTKKTSAEEVMQTVDRHLGAIARPKAVHFVALLPKTRSGKTLRRSIQ
                     ALAEGRDPGDLTTIEDPNALEQIRQALR"
     gene            738..1136
                     /locus_tag="VIG70_00950"
     CDS             738..1136
                     /locus_tag="VIG70_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY8249144.1"
                     /translation="MKNRPMLTLEDCRKITAAAEAEAKKNNWNVCIAILDDGGHLLHL
                     ARMDGATPANARIAVEKGRTAAESRRSTAVWQERIKPRPEMLRMPGITPIQGGLPIVV
                     EGTCVGGVGISGVQSHEDEQIAKAGIDALK"
     gene            1144..1998
                     /locus_tag="VIG70_00955"
     CDS             1144..1998
                     /locus_tag="VIG70_00955"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8249145.1"
                     /translation="MPGKQAAWIGLGLIGLPMAARLAAAGWQVKGFDISKERLEMAAA
                     RGISAVASAEAAIEGASLVFTSMPTESALIELAKKLAAGMPKPAILVETTTAGPAASA
                     KVAEALAARRIAYLRGPISGSVNLAETGALTTFVSGPKAAFDAAKPALAAYTRAQIWL
                     GEAEEARYAKLAVNLMVAVTAGMLRETLALAEQGGIGWEAALDLLGESVVASPLVKYK
                     LEPLRRRDYTPTATGALVLKDLDLIVETARQAGIEVPLAREVRAAYQRMAKAQLAGED
                     FISIVKRP"
     gene            2000..3199
                     /locus_tag="VIG70_00960"
     CDS             2000..3199
                     /locus_tag="VIG70_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY8249146.1"
                     /translation="MRVLIAGAGIGGLTAALAFLKRDIDVEVHEQAAELREVGAGIQL
                     SANGTRAFYALGVGDELKALSAETASKELHLWNTGETWRIFDLGKVSVERYGYPYFTV
                     YRPDLLAVLAAAVRREKPDAIRLGSKCTGFSQDSTEVRLRLESGEARGDALVGADGVH
                     SQIRQGLFGQDQPQVTGIIVWRGIVPMERLPKHMARMVGHNWVGPGGHVVHYPLRAGK
                     VMNFVGALERSDWQVESWSARGTTGELSADFEGWHEDIQALIRGIPVPYKWALMTRPP
                     MERWTVGRVTLLGDACHSMVPFLAQGAVMAVEDGVVLARALRELDGDIPSRLARYENA
                     RRERTRGAVEGSADNIGRFHNRALADPAKARDYVEREWAGQNVASRYEWLFKYDVTAA
                     GLFGENS"
     gene            3196..4143
                     /locus_tag="VIG70_00965"
     CDS             3196..4143
                     /locus_tag="VIG70_00965"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8249147.1"
                     /translation="MRADAQLISALERANIHPLWDRYQRITPLAPRAKDAPMHWRWRD
                     IEPFAERAAKEVAIEDVERRALILAHPAFGGATVTTQNLIGAFTVLEPGDKAVPHRHT
                     AAAIRFSTRAEGAVTIVNGRRCEMEEGDLVLTPPMCWHGHINQSGRRTVWFDAANMPA
                     VCALDASFFEPGTRQDERFWEVGDAGSADYRFPGTETRKEIASTALGADGARTVRYTK
                     DGGSVMPTLDLFFARIEKRTRPKRGTYNAICLVVSGEGRSTIGEHRFDWERHDVFTVP
                     HWTWASHEASVGKSGAAELFVVTDRAIYERLDLLREELG"
     gene            4140..6362
                     /locus_tag="VIG70_00970"
     CDS             4140..6362
                     /locus_tag="VIG70_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8249148.1"
                     /translation="MIGSPVRRVEDLRFLTGEGRFIDDFAFPGMLYCALVRSPHAHAR
                     IRAIQFSKELLVLTGRDMEADGVGPMRAGWQIPGMVEPARWALARETVRHVGEPVAAV
                     FAETRALAEDAAERVEVDYEFLPLIHEETAFRWTRGDAAAVDAAFASAAHRVDLELVN
                     NRLCGAAIEARGVLSTSDTLYCATQAPHHIRQHVCAELDLREIDLRVVSPDMGGGFGY
                     KGKHYPEETIAVWAARRLRRPVKWVATRQESFLSDTQGRDHRTRAALALDDEGGFLAL
                     RVGTQADLGAYVSTFGAAIAGPIYSALLAGLYRTPAIHVEVTGMFSNSVPTDAYRGAG
                     RPEACYVLERLADKAARELGLDRAEIRRRNLIPKEAMPYKTPIGPTYDCGDFPRIFER
                     ALAVSDYAGFEARRKSSKKRRGIGIACYLESSGVAPSRLAGMMGARVGFYEAATVRVA
                     PDASVTAYLGTHNHGQGHATTFAQIVAERFALPLERISIVEGDTAMVPVGTGTFGSRS
                     LAVGGSALHVACQKIILKGKKIAAHLLEASAADIEFSAGEFHVGGTDRKIGFAQVAGA
                     AYVPHNYPLETLEPGLDESAFYDPPNFAFSNGVHVCEVEVDPETGAAEVVSYHAVDDI
                     GTVINPMIVEGQVHGGLAQGIGQALAERTVYDAGQLVSGSFMDYALPRAGDLPRFHSE
                     TDESQPCTHNPLGAKGCGEAGAIGAPPAVMNALLDALGVADLEMPATPHRLWQAIMGK
                     "
     gene            6359..7420
                     /locus_tag="VIG70_00975"
     CDS             6359..7420
                     /locus_tag="VIG70_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249149.1"
                     /translation="MIQELNPAKLALLYRRQFELCKVKKGETIAAVSDLATRREYLQA
                     AFAAADDLGADIYELCVNSLPSWTKVGVPTIGKAKGTLEAVKAADLVVIFHVPLFTKW
                     LKQVMDGGTRVLMIIDSPDDLEQLMAPAGLKGACKHADSLYRKTKRVRVRSEAGTDLT
                     YDCGEYPVMTQWGFADERGHFDHWGGGHIHTFPNEGSATGTVVFQPGDIVILPYCRYI
                     ADPVKLEIREGHIVKLEGGIDAKLMRDWLEDGRSGPKDRDPYAVSHLGWGLNPQALWY
                     GIALHGDAPERHRAAARTFPGNFLFSTGPNTQGGGKRDTRGHYDVPMRDCTVELDGKV
                     VVDRGRIVDEKMKVTRETR"
     gene            7417..8577
                     /locus_tag="VIG70_00980"
     CDS             7417..8577
                     /locus_tag="VIG70_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8249150.1"
                     /translation="MKKLLLAIALLCLAPVQAANKVKIGFIGTLSGPNASVGVDIRDG
                     FQLAIKLNGGKLGGLPAEVLIGDDGLKPEQAKSLAERYLRLEKVDFVTGTVFSNVVVA
                     IAPEILAAKTFYIAPNAGPAPYSGAQCNPFFFVSSWPSEAYSEAAGQYITSKGIKNVL
                     FLAPNYQGGKDAATGFKRYYKGKLVDEMYTKLGQLDYAAELSQIRAAKPEALYVFLPG
                     GMGVNFIKQFVAAGMSKDIQLVVPMWGSDQDIIRAVGDPMLGLFSVGHWSLDFDNPAN
                     RKFVAEFEKEYKRLPTGYAASGYDTALLMDAAIRKVKGKIEDKDALRAALRAAEFKSV
                     RGDFKFGPNQFPVQNYYLQLVGKGNDGRIVHKTIGTILQNRADAYVQDCKMK"
     gene            complement(8585..9055)
                     /locus_tag="VIG70_00985"
     CDS             complement(8585..9055)
                     /locus_tag="VIG70_00985"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY8249151.1"
                     /translation="MSPVRISLGFAFAAFATFAAAQGRPDYGPSITLATAKKVAAATI
                     AECQKNKWNVAVAVVDTHGALLYFERLENTQYASNDIAIGKARAAATYQRPTRAFVDA
                     IAKAGPAVLSLGAIYASPGGVPLMADGKVIGAVGVSGVTGDQDEQCAKAGASGM"
     gene            complement(9082..10212)
                     /locus_tag="VIG70_00990"
     CDS             complement(9082..10212)
                     /locus_tag="VIG70_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY8249152.1"
                     /translation="MVMGPSCGLVLADLGAEVIKVEPLAGDNTRRLEAAGAGFFPVFN
                     RNKKSLAVDLQKPEGRDLVLRLLKKADVLTENFRPGALDKLGFSYEKLSKENPRLVYC
                     SLKGFLKGPYEHRLALDEVTQMMGGLAYMTGLPDRPLRAGSSVIDIMGGTFAAVGILA
                     ALREREKTGRGAQVTSALYESTAYLVAQHMAQFEITGEAPLPMSVKRPAWGIYDIFDT
                     LGGGRLFVGVVTDTQWEVFCRAFNAQDLAGDARLRSNGQRVKERAWLVPRINEITKKL
                     SQDELAAKLEAIGLPFAPIAKPWDLLDDPHLNASEGLLETRINSKTIRVPALPLALDG
                     KRLRKRTDPPRIGEHGRELLAALGCSPQEIETLRDRRIVALP"
     gene            complement(10241..11155)
                     /locus_tag="VIG70_00995"
     CDS             complement(10241..11155)
                     /locus_tag="VIG70_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY8249153.1"
                     /translation="MAAMENVHICEVGPRDGLQNAHHLMPTEAKKAWIAALAAAGLKE
                     IEVGSFVPPKLIPAMADTGEIVAFAKTLKGVKVVALAPNLKGFQRALEAGAHKVTFPV
                     SASRKHSESNVRMTPEQMVEEVRKCAQHPHPGVEIEGAVSTAFGCTLQGEVPEDDVVR
                     IAVALADFCDFVCLADTVGYANPAQIRRLFRKVRDEIGAKLEAAHLHNTRGLGLANAL
                     AAYEEGVRHFDSSMGGLGGCPFAPGASGNVITEDLVFMFESMGVRTGVDFQKLLAARE
                     LLVKAIPDEPVYGHTPLAGLPKGFRAAA"
     gene            complement(11157..12329)
                     /locus_tag="VIG70_01000"
     CDS             complement(11157..12329)
                     /locus_tag="VIG70_01000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8249154.1"
                     /translation="MTNWPAVWVIFAGGLAAGAYMTKVPPALPTMRADLGLTLVESGL
                     IQTMLYVIGASVGVFFGVFGERIGVKRAALAGLGVMVAGGLVGALAGGYGTLLASRFL
                     EGVGFLLFVVAAAPLLAAATLPQDRATAFSIWSSYMPTGGTLALLVAPLVLSGFGWRG
                     LWVALAAYAALCAVLLARKVPPAQFGSGIGSLRLLAESLARPGSLALCVGFICYVGQW
                     TSLMTWLPTFVVDERGASPSAAALVTAAFVAINIPGNQLGGLLLRAGLPRWLVMGAGA
                     FAMGATALGFFSTALPDTLRLACVMAFSLLGGVIPAAVFSGTTVHAKSPQHIGTTNGM
                     VMQASHGAQFAIPILIAWVASRAGGWGASLQVMAALALVGIGAAVAVGRFERRLGK"
     gene            complement(12326..13846)
                     /locus_tag="VIG70_01005"
     CDS             complement(12326..13846)
                     /locus_tag="VIG70_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016725283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEY8249155.1"
                     /translation="MTTITRPHLVQSVADALQFISYYHPADYIRALGRAYEREQSAAA
                     KDAIAQILTNSRMCAEGHRPICQDTGIVNVFLKIGMQVRFDFDDLEAAVNEGVRRAYL
                     DPDNKLRASVLDESDFGRKNTKDNTPAVISVQMVPGDKLEVDVAAKGGGSEAKAKFAM
                     LNPSDSIAEWVLKTVPTMGAGWCPPGMLGIGVGGTAEKAMLMAKEALMEPIDMAELKA
                     RGARNKVEELRVELYDKVNALGIGAQGLGGLSTVLDVKIRTYPVHAANRPVAIIPNCA
                     ATRHAHFVLDGSGPAEFEEPKLSDWPAVTWKPDSASKRVSLDTLTRAEVASWKPGQRL
                     LLSGKLLTGRDAAHKRIQDFLEQKKPLPVDFTNRVIYYVGPVDPVRDEVVGPAGPTTS
                     TRMDKFTEMMLAKTGLVAMVGKAERGPAAIEAIRKHKAAYLMAVGGAAYLVSKAIRKS
                     RVLAFEDLGMEAIYEFEVEDMPVTVAVDASGTSVHTTGPAEWSKKIVELKIPVTAA"
     gene            complement(13945..14061)
                     /locus_tag="VIG70_01010"
                     /pseudo
     CDS             complement(13945..14061)
                     /locus_tag="VIG70_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
     gene            14129..16102
                     /gene="acs"
                     /locus_tag="VIG70_01015"
     CDS             14129..16102
                     /gene="acs"
                     /locus_tag="VIG70_01015"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY8249156.1"
                     /translation="MATSTIESVLQEKRVFPPAAAIVKQANISGMEAYRKLVAEFERD
                     FQGTWGRLARETLHWSKPFTKVLDESKAPFFRWFHDGELNASYNCLDRHLKTQPDKTA
                     IIFEADDGKVTRITYQELYKEVCKLANALKAKGIKQGDRVLIYMPMSIQAVVAMQACA
                     RIAATHSVVFGGFSAKSVQERIIDAGAIAVITADGQFRGGREIPLKPVVDEALGMGGC
                     EGIKNVIVYQRSGSKVPMQAGRDTWWDDAVKGQADTCAPTFVNAEHPLFILYTSGSTG
                     KPKGIQHSTGGYLLWCALTMKWVFDSKPGDVFWCTADVGWVTGHSYIAYGPLAVGATE
                     VVFEGVPVYPDAGRFWKVIQDHKVNVFYTAPTAIRSLIKAGGDLPKKYDLSSLRILGT
                     VGEPINPEAWMWYHETVGGGRCPIVDTWWQTETGGHLISPLPGATPTKPGSATLPLPG
                     IFADIVDETGHSVGRGKGGILVITRPWPSMLRTIWGDPERYKKTYWPDDFKGKLYLAG
                     DGASMDEEGYFRIVGRIDDVLNVSGHRLGTMEVESALVANSKLVAEAAVVGKPDELTG
                     EAICAFVVLKGPRPNGAEAKRIAEELRQWVAKEIGPIAKPKDIRFGDNLPKTRSGKIM
                     RRLLRSIAKGEEIKQDVSTLENPQILEQLKQST"
     gene            16235..16432
                     /locus_tag="VIG70_01020"
     CDS             16235..16432
                     /locus_tag="VIG70_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249157.1"
                     /translation="MEEFDVRGQRLAALCLLGLLLFSYPVLAVFNVQATVAGIPVLYA
                     YFFVAWGALIGLVALVIERTP"
     gene            16445..19135
                     /locus_tag="VIG70_01025"
     CDS             16445..19135
                     /locus_tag="VIG70_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY8249158.1"
                     /translation="MLETWVVIVTAFAYLGLLFAIAYYADQRADAGRSVIASPYIYSL
                     SLAVYATAWTFYGSVGRAAENGVGFLPIYIGPTLMIALWWVVMRKILRISKQNRITSL
                     ADFIASRYGKSALLGGVVTVIAVIGILPYISLQLKAVSVSFTTVAGAVPGLGDTALWV
                     ALILAAFTIAFGTRHLDAAEHHEGMVAAIAFESLVKLLAFLAVGAWVSWGLYNGFGDI
                     FARAAAEPELRPMLAPFGGVGGDYSSWIWLTLLSMLAIMFLPRQFQVTVIENKDESHL
                     KKAIWLFPLYMLAINIFVLPIAFGGLLHFPSGSVDADSFVLTLPLSQKQELLALVVFI
                     GGLSAATGMVIVETIALSTMVCNDLVMPVLLRMRALRLNQRRDLTGLLLGIRRGAIVL
                     ILLLGYLYFKVAGEAYALVSIGLISFAAVAQFAPAALGGIFWKGGTRRGALAGLGAGF
                     AVWFYTLLLPAFARSDWLPIGFLEHGPFGFELLKPVALFGVSGLDQITHAMIWSMVAN
                     VGAYVGVSLMRSPSAEEHRQATLFVDVFKRPAGARLRRGTASEAELFNLLARFVGLRA
                     ASDAFAEHARRGADADLVQFVERQLAGVIGAASAHIMLAAVVKEEALTLDEVRAVLDD
                     ASQIVVYSHRLEQKSQELERATQELRAANERLTELDRMKDEFVSTVSHELRTPLTSIR
                     SFTEILHDNPQLPAAERSRFLGIVIKETARLSRLIGQVLDVSRLESGRVEWHESLLDM
                     REVIEDTAASTSQLFRERNVRLERNLPGRVPKVRADLDRIVQVVVNLLSNAVKFIEPG
                     RGRVEIALAEEAGFLRVDVRDNGPGISPEHQEMIFERFRQAGDPLTGKPQGTGLGLYI
                     SRRIIEHSGGRLWVSSRPGEGACFSFTLPLVAEASLGRAA"
     gene            19132..19752
                     /locus_tag="VIG70_01030"
     CDS             19132..19752
                     /locus_tag="VIG70_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8249159.1"
                     /translation="MTAKPAPTAYVVDDDESIRSLWQWLLGSNGIAVQTFATATAFID
                     AYRRGDAGCLVLDVRLPGMSGPELQDHLRREAIEIPIVFVSAHGDVPTAVNAIKGGAV
                     DFIQKPFDYREALAIVRRALERDAQVRERRARQTELAGRFAALTAREREVLHRIIEGK
                     SNKVIAEELDISVRTVEFHRANVMEKTGAESVGALIQQMALHSGLE"
     gene            19857..20123
                     /locus_tag="VIG70_01035"
     CDS             19857..20123
                     /locus_tag="VIG70_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEY8249160.1"
                     /translation="MELTEKHREYWRKNLVITAILLAVWFVVTFVEGWYARELNNISF
                     LGFPLGFYMSAQGSLIIYVVIIGIYARYMNNLDKVYDVHEGEDE"
     gene            20120..>21367
                     /locus_tag="VIG70_01040"
     CDS             20120..>21367
                     /locus_tag="VIG70_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HEY8249161.1"
                     /translation="MSAVYSHKSFNSQLKKYYSVYTGGFIAFVIALAIAEQIGMSRAW
                     IGYWFLFATIALYAAIGIMSRTVDAAEYYVAGRRVPAFFNGMATGADWMSAASFIGMA
                     GTLYLSGYDGLSFVMGWTGGYVLVALFLAPYLRKFGQFTIPDFLGARYGGNIVRSIGI
                     FAAILCSFTYVVAQIYGVGIITSKFTGLEFGIGVFVGLAGILVCSFLGGMRAVTWTQV
                     AQYIILIIAYLTPVVWLGVKHTGIPIPQFAYGYTLQKVGDLEKKITADPKEVEARGLA
                     KAAAAEASAALKDPVKAAAERRAALEQRLAKAKADNAPDHVQKSIQQALSNTPTDPAK
                     AQEYWSARRNLGANANPPRKHADPFPGATEEARHTAKLNFLGIVFCMMFGTAALPHIL
                     MRYYTTPSVRQARTSVFWSLFFIF"
BASE COUNT         3608 a         7017 c         7286 g         3456 t
ORIGIN      
        1 gaggcgctcg gccggccggt gatcgaccac tactggcaga ccgagaccgg ctggccgctg
       61 ctctcggcgc acccgggcgt ggagaagacg ccgctcaaga tgggctcgcc gagcttcccg
      121 tgctacgact acgacgtgcg cttgctgcat gaaggctcgg gcaaggaagt caaagacggg
      181 gaaaaaggcg tcgttacggt cgtgccgccg ctgccgccgg gctgcatgtc gaccgtgtgg
      241 ggcgacgacg agcgcttcgt caatacctac ttcaacgact tcaaaggcca gcaggtctac
      301 tcgaccttcg actggggcat ccgtgaccag gacggctact acttcatcct tggccgcacc
      361 gacgatgtga tcaacgtcgc cggccatcgc ctcggcacgc gcgagatcga ggaggccgtg
      421 agcatgcacc ccaacatcgc cgaatgcgcg gtggtaggcg tggcggacgc gctcaagggt
      481 cagatgcccc tggcttttgc cgttctcaag gattccacca aaaagacttc cgcggaagaa
      541 gtcatgcaaa ccgtcgaccg acacctcggt gccatcgcgc ggcctaaagc ggtgcacttc
      601 gtcgcgctgc tgcccaagac gcgctcgggc aagacgttgc gccgctcgat ccaggcgctc
      661 gccgaaggcc gcgacccggg cgacctcacc accatcgaag atcccaacgc actggagcag
      721 atccgacagg ccctgagatg aaaaaccgac ccatgctgac gctggaggat tgccgcaaga
      781 tcaccgccgc cgccgaagcc gaggcgaaga aaaacaactg gaacgtgtgc atcgcgatcc
      841 tcgacgacgg cgggcacctg ctgcacctcg cacgcatgga cggcgcgacg ccggccaacg
      901 cacgcatcgc ggtggaaaag ggccgcaccg cggccgagtc gcgccgctcg accgccgtct
      961 ggcaggagcg catcaagccg cggcccgaga tgctgcgcat gccggggatc acgccgatcc
     1021 agggcggcct gccgatcgtg gtggaaggca cctgcgtggg cggggtcggg atctccggcg
     1081 tgcagtcgca cgaggacgag cagatcgcca aggccggcat cgacgccctg aagtaggtcc
     1141 gtcatgcccg ggaaacaagc agcctggatc ggtctcggct tgattggcct gccgatggcg
     1201 gcgcggctcg cagcggcggg ctggcaggtg aaaggcttcg acatcagcaa ggagcgcctc
     1261 gagatggcgg ccgctcgcgg aatcagtgcc gttgcctcgg ccgaagccgc gatcgaaggc
     1321 gcgagcctcg tcttcacttc gatgccgacc gagtccgccc tgatcgagct tgcgaagaag
     1381 cttgcggccg gcatgccgaa gcccgcgatc ctggtggaga caaccaccgc gggtccggca
     1441 gcctcggcga aggtggccga agcgctggct gcgcggcgca tcgcctacct gcgagggccg
     1501 atctcgggca gcgtcaacct cgcggagacc ggtgctctga cgactttcgt gtccgggccg
     1561 aaggccgcgt tcgacgccgc gaaaccggca ttggcggcct acacgcgcgc gcagatatgg
     1621 ctgggcgagg ccgaggaagc ccgctatgcc aagctcgcgg tgaatctcat ggtggcggtg
     1681 accgccggca tgctgcgcga gacgctcgcg ctcgcggagc agggcggcat cggctgggaa
     1741 gcggcgctcg atctgctcgg cgagagcgtg gtcgcgtcgc cgctggtgaa atacaagctc
     1801 gagccgctcc ggcggcgcga ctacaccccg acggcgaccg gcgcgctggt gctgaaggac
     1861 ctcgacctca tcgtggagac ggcgcggcag gcgggcatcg aggtgccgct cgcccgcgag
     1921 gtgcgcgcgg cctaccagcg catggcgaag gcgcagctcg ccggcgagga tttcatcagc
     1981 atcgtgaaac gcccctagct tgcgggtcct gattgccggc gccggaatcg gtggactgac
     2041 ggccgcgctt gctttcctca agcgcgatat cgatgtggaa gtccacgagc aggcggccga
     2101 gctgagggag gtcggcgccg gcatccagct cagcgcgaac ggcacgcgcg cgttctacgc
     2161 cctcggcgtc ggcgacgagc tgaaggcgct ttccgccgag accgccagca aggagctcca
     2221 cctctggaat accggcgaga cctggaggat cttcgatctc ggaaaagtgt ccgtcgagcg
     2281 ctacggctat ccgtacttca cggtctaccg tccggacctg ctggccgtcc ttgccgccgc
     2341 cgtgcgtcgc gagaagccgg atgcgatccg cctcggctcg aagtgcacgg gtttcagcca
     2401 ggattcaact gaagtgagac ttcgactcga aagcggtgaa gcgaggggcg atgccctggt
     2461 cggcgccgac ggcgtgcact cgcagatccg ccagggactc ttcggccagg accagccgca
     2521 agtcaccggg atcatcgtct ggcgtgggat cgtgccgatg gagcggctgc caaagcacat
     2581 ggcgcgcatg gtcgggcaca actgggtcgg gccgggcggg catgtggtcc actatccgct
     2641 gcgggccggc aaagtgatga atttcgtcgg cgcgctggag cgcagcgact ggcaggtcga
     2701 gtcctggagc gcgcgcggca cgaccgggga gctgtcggcc gacttcgaag gctggcacga
     2761 ggacatccag gcgctgattc gcggcatacc ggtgccctac aaatgggcgc tgatgacgcg
     2821 cccgccgatg gagcgctgga ccgtgggccg ggtcacgctg ctcggagacg cctgccactc
     2881 catggtgccg tttcttgccc aaggcgcggt catggccgtc gaggatgggg ttgtcctcgc
     2941 acgagccctg agggaactcg atggggacat tccgtcccgc ctcgcgaggt acgaaaacgc
     3001 gaggcgcgag cgcacgcggg gcgcggtcga aggctcagcc gacaacatcg ggcgctttca
     3061 caaccgcgcg ctcgccgacc cggcgaaggc gcgcgactac gtcgagcgcg agtgggccgg
     3121 acagaacgtg gcctcgcgct acgagtggct gttcaaatac gatgtgaccg ccgcaggact
     3181 attcggagag aactcatgag agccgatgcc cagctgatct ccgcactgga gcgagccaac
     3241 atccacccgc tctgggaccg ctaccagcgc atcaccccgc tcgcgccgcg agcgaaggac
     3301 gcgccgatgc actggcgctg gcgcgacatc gagccttttg ccgagcgcgc ggcgaaggaa
     3361 gtggcgatcg aagacgtgga gcgccgcgcg ctgatccttg cgcatcccgc ctttggcggc
     3421 gcaaccgtca ccacgcagaa cctgatcggc gccttcacgg tgctcgagcc gggagacaag
     3481 gccgtgccgc accgccacac cgcggcggcg atccgtttct ccacgcgcgc cgaaggcgcg
     3541 gtcacgatcg tcaacggcag gcgctgcgag atggaggagg gcgacctcgt gctgacgccg
     3601 ccgatgtgct ggcacgggca catcaaccag agcggccggc gcacggtgtg gttcgacgcc
     3661 gccaacatgc cggcggtctg cgcgctcgac gcgagcttct tcgagcccgg cacgcgccag
     3721 gacgagcgct tctgggaggt cggcgacgcg gggtcggccg actatcggtt ccccgggacc
     3781 gagacccgga aagagatcgc gtccaccgcg ctcggcgccg acggcgctag aaccgtgcgc
     3841 tatacgaaag acggcggctc ggtcatgccc acgctggacc ttttttttgc gaggatcgaa
     3901 aagcgaacga gaccgaagcg aggcacgtac aacgcgatct gtctcgtcgt gtccggcgaa
     3961 ggccgctcga ccataggcga gcaccgcttc gactgggaac gccacgacgt cttcaccgtg
     4021 ccgcactgga cctgggcgag ccatgaagcc tcagttggca aatcgggcgc ggcggagctg
     4081 ttcgtggtca ctgaccgcgc gatctacgag cgcctggatc tcctgcgaga agagctgggg
     4141 tgatcgggtc cccggtccgg cgggtggagg acctgcggtt cctcaccggc gagggccgtt
     4201 tcatcgacga ctttgccttc ccggggatgc tttactgcgc cttggtccgc tcgccgcacg
     4261 cgcatgcccg cattcgagcc attcaattct caaaagaatt gctggtgctg acgggccgcg
     4321 atatggaggc ggacggtgtc ggcccgatgc gagccggatg gcagatcccc ggcatggtgg
     4381 agccggcgcg ctgggcgctc gcgcgcgaga ccgtgcggca tgtcggcgag ccggtcgccg
     4441 cggtgtttgc ggaaacccgg gcgcttgccg aagacgccgc cgagcgggtc gaggtggact
     4501 acgaattcct gcccctcatc catgaggaga ccgctttccg ctggacgcgg ggcgacgccg
     4561 cggcggtcga tgcggcgttc gcgagcgccg cgcatcgcgt cgacctcgag ctggtgaaca
     4621 accgcctgtg cggggccgcg atcgaggcgc gcggcgtgct ctcgacaagc gacacgctgt
     4681 attgcgccac gcaggcgccg catcacatcc ggcagcacgt gtgcgcggag ctcgacctgc
     4741 gagagatcga cttgagggtg gtctcgcccg atatgggcgg cggcttcggg tacaagggaa
     4801 agcactaccc ggaggagacc atcgccgtct gggcggcgcg ccgcctgcgc cggccggtca
     4861 agtgggtcgc gacgcgccag gagtccttcc tttccgacac gcagggcagg gaccaccgca
     4921 cgcgcgccgc cctcgccctg gacgacgagg gcggcttcct cgctctccgc gtcggcacgc
     4981 aggccgatct cggggcctac gtctcgacct tcggcgcggc gatcgcgggg ccgatctaca
     5041 gcgcgctgct ggccgggctc tatcgaacgc cggccatcca cgtcgaagtc accgggatgt
     5101 tctcgaacag cgtccccacc gacgcgtatc gcggcgccgg ccggccggag gcctgctacg
     5161 tgctcgagcg gctcgcggac aaggcggcgc gcgagcttgg cctcgaccgc gccgagattc
     5221 gccggcgcaa cctcatcccg aaggaggcga tgccgtacaa gactccgatc ggtcccactt
     5281 acgactgcgg cgatttcccc aggatcttcg agcgcgcgct tgcggtttcg gattacgcag
     5341 gattcgaagc cagacgaaaa tcctcgaaga agcggcgcgg catcggtatc gcgtgctatc
     5401 tggaatcgtc cggcgtggcg ccctcgcgtc tcgcgggcat gatgggcgcg cgcgtcgggt
     5461 tctacgaagc ggcgacggtg cgcgtcgcgc ctgatgcgag cgtgaccgcc tacctgggca
     5521 cgcacaacca tggccagggc cacgcgacga cgttcgcgca gatcgtcgcc gagagatttg
     5581 cacttccgct ggaaagaatc tcaatcgtcg agggcgacac ggccatggtc ccggtcggca
     5641 ccggcacgtt cggctcgcgc tcgctcgccg tcggcggctc ggcgcttcac gtggcctgcc
     5701 agaagatcat cctgaagggc aagaagatcg ctgcgcacct gctggaggct tccgcggccg
     5761 acattgagtt ctcggcagga gagttccacg tcggcggaac ggatagaaaa atcgggttcg
     5821 cgcaggttgc cggcgctgcc tatgtgccgc acaactatcc gctcgagacg ctggagcccg
     5881 gcctggacga gagcgcgttc tacgatccgc cgaacttcgc gttctccaac ggcgtgcatg
     5941 tctgcgaggt ggaagtcgat cccgagaccg gcgcggccga ggtggtctcg taccacgcgg
     6001 tcgacgacat cggcaccgtg atcaacccga tgatcgtcga ggggcaggtg cacggcgggc
     6061 tcgcccaggg gatcgggcag gcgctcgccg aacgcacggt gtacgacgcg ggccagctcg
     6121 tttcggggtc cttcatggac tacgcgctgc cgcgcgccgg cgatctgccg aggtttcatt
     6181 cggaaaccga cgagtcccag ccctgcacgc acaacccgct cggcgccaag gggtgcggcg
     6241 aagcaggggc catcggcgcg ccgccggcgg tgatgaacgc gctcctcgat gcactcggag
     6301 ttgccgacct ggaaatgccg gctacaccgc accgactctg gcaagccata atgggcaaat
     6361 gattcaggag ctgaatccgg ccaagctggc gctgctctac cgccggcagt tcgagctgtg
     6421 caaggtgaag aagggcgaga ccatcgccgc ggtcagcgac ctcgccacgc gccgcgaata
     6481 tctgcaggcc gcgttcgccg ccgccgacga tctgggcgcc gacatctacg agctgtgcgt
     6541 gaactcgctg ccgtcgtgga ccaaggtcgg cgtgccgacg atcggcaagg ccaagggcac
     6601 gctggaagcg gtcaaggcgg cggacctggt ggtgatcttc cacgtgccgc tcttcacgaa
     6661 gtggctgaag caggtgatgg acggcggcac gcgcgtgctg atgatcatcg attcgcccga
     6721 cgacctcgag cagctcatgg cgcccgcggg gttgaagggc gcttgcaaac acgcagacag
     6781 cctctatagg aagacgaagc gcgtccgcgt gcggagcgaa gcgggtacgg acctcactta
     6841 cgactgtggc gaatacccgg tgatgacgca gtggggcttc gccgacgagc gcgggcattt
     6901 cgaccactgg ggcggcgggc acatccacac gtttccgaac gaaggcagcg cgaccggcac
     6961 cgtggtgttc cagccgggcg acatcgtgat cctgccgtat tgccgctaca tcgccgaccc
     7021 ggtgaagctc gagatccgcg aggggcacat cgtgaagctg gagggcggca tcgacgccaa
     7081 gctgatgcgc gattggctgg aagacggaag atcggggccg aaggaccgcg atccctatgc
     7141 cgtgtcgcac cttggctggg gcctgaaccc gcaggcgctc tggtacggca tcgcgctgca
     7201 cggcgacgcg ccggagcgcc accgcgcggc ggcgcgcacg ttcccgggca acttcctgtt
     7261 ttccaccggg ccgaacacgc agggcggcgg caagcgcgac acgcgcgggc actacgacgt
     7321 gccgatgcgc gactgcaccg tcgagctcga cggcaaagtg gtggtcgatc gcggacggat
     7381 cgtcgacgaa aaaatgaaag tcacccggga gacaagatga agaagctatt gctggcgatt
     7441 gcgctgctgt gccttgcgcc ggtgcaagcg gcgaacaagg tcaagatcgg cttcatcggc
     7501 acgctgtcgg ggccgaacgc ttcggtcggc gtcgatatcc gcgacggctt ccagctcgcg
     7561 atcaagctga acggcggcaa gctcggcgga ttgcccgccg aggtgctgat cggcgacgac
     7621 ggcctcaagc ccgagcaggc gaagagcctg gccgagcgct acctccggct ggaaaaggtg
     7681 gacttcgtga ccggcacggt gttttccaac gtggtggtcg cgattgcccc ggagatcctc
     7741 gccgccaaga cgttctacat cgcgccgaac gcgggcccgg cgccgtactc cggcgcgcaa
     7801 tgcaacccgt tcttcttcgt ctcgtcctgg ccgagcgaag cgtacagcga ggcggcaggg
     7861 cagtacatca cgtccaaggg catcaagaac gtgctgttcc tcgcgccgaa ctaccagggc
     7921 gggaaggacg ccgccaccgg cttcaagcgc tactacaagg gcaagctggt cgacgagatg
     7981 tacaccaagc tcggccagct cgactacgcg gcggagctgt cgcagatccg cgcggcgaag
     8041 cccgaggcgc tctacgtctt cctgcccgga ggcatgggcg tcaacttcat caagcagttc
     8101 gtcgccgccg gcatgtcgaa ggacatccag ctcgtcgtgc cgatgtgggg ctcggaccag
     8161 gacatcatcc gcgccgtggg cgatccgatg ctgggcctct tcagcgtcgg gcactggtcg
     8221 ctcgacttcg ataacccggc caaccgcaag ttcgtcgccg agttcgagaa ggagtacaag
     8281 cggctcccga ccggctatgc cgcgtcaggc tacgacactg cgctcctcat ggacgccgcg
     8341 atccgcaagg tgaagggcaa gatcgaggac aaggacgctt tgcgcgctgc gctgcgtgcc
     8401 gcggagttca agagcgtgcg cggcgacttc aagttcggcc cgaaccagtt cccggtgcag
     8461 aactactacc tgcagctggt cggtaaagga aacgacgggc gcatcgtgca caagacgatc
     8521 ggcacgatcc tgcagaaccg tgccgacgcc tatgtgcagg actgcaagat gaaataaggc
     8581 gggcttacat cccgctggcg ccggccttgg cgcactgctc gtcctggtcg ccggtgacgc
     8641 cgctcacgcc gaccgcgccg atgaccttgc cgtccgccat gaggggcacg ccgccgggcg
     8701 aggcgtagat ggcgcccagg ctcaacactg cgggccctgc cttggcgatc gcatcgacga
     8761 acgcgcgcgt cgggcgctga taggtcgccg cggccctggc cttgccgatg gcgatgtcgt
     8821 tgctcgcata ttgcgtgttc tccaaccgct cgaaatacag gagcgcgccg tgcgtgtcga
     8881 cgactgcaac ggcgacgttc cacttgttct tctggcactc ggcgatggtt gccgccgcga
     8941 ccttcttggc cgtcgcgagg gtgatggacg gaccgtagtc cggccgtccc tgggctgcgg
     9001 caaaggtggc gaatgctgcg aaagcaaagc caagcgagat cctgacaggc gacatctgtt
     9061 tctcctctgg tagtagtgag actagggcag cgcgactata cgtcggtcgc gcagggtttc
     9121 gatctcctgc ggtgaacagc cgagcgcggc gagcaattcc ctgccgtgct cgccgattcg
     9181 cggcgggtcg gtgcgcttgc gcaggcgctt gccgtccagc gccagcggca gcgcgggcac
     9241 ccgaatggtt ttcgaattta ttcttgtttc tagaagccct tccgacgcgt tgagatgggg
     9301 atcgtcgagc aggtcccagg gcttggcgat cggcgcgaac ggcagcccga tcgcctcgag
     9361 ctttgcggcg agctcgtcct gcgagagctt cttcgtgatc tcgttgatcc gcgggaccag
     9421 ccaagcgcgc tctttcaccc gttggccgtt gctgcgcagc cgcgcgtcgc cggccaggtc
     9481 ctgagcgttg aacgcccggc agaaaacctc ccattgggtg tcggtcacga cgccgacgaa
     9541 gagccgcccg ccgccgaggg tgtcgaaaat gtcgtagatg ccccacgccg ggcgcttcac
     9601 cgacatcggc agcggcgcct cgccggtgat ctcgaactgc gccatgtgct gcgcgacgag
     9661 atacgcggtg ctttcgtaga gcgcgctggt gacctgcgct ccgcggccgg tcttctcgcg
     9721 ctcgcgcagc gcggcgagga tgccgaccgc ggcgaacgtg cctcccatga tgtcgatcac
     9781 cgacgagccg gcgcgcagcg ggcggtcggg aagcccggtc atgtaggcga gcccgcccat
     9841 catctgcgtc acttcgtcga gggcgaggcg gtgctcgtag gggcccttga ggaagccttt
     9901 cagcgagcag tagaccagcc gcgggttttc cttcgacaac ttctcgtagg agaagccgag
     9961 cttgtcgagc gcgccgggcc ggaagttctc ggtgaggacg tccgcttttt tcaggagccg
    10021 gagaacgagg tcgcggcctt cgggcttctg caaatcgacc gcgaggcttt tcttgttgcg
    10081 gttgaagacc ggaaagaatc cggcgcccgc ggcttcgagc cgccgcgtgt tgtcgccggc
    10141 gagcggctcg accttgatca cctcggcgcc gaggtcggcg agcaccagcc cgcaggaggg
    10201 gcccatcacc atgtgcgcga attcgactac gcgcacaccc tcaagcggca gcacgaaatc
    10261 ctttgggcag tcctgcgagc ggcgtgtggc cgtacacggg ctcgtcggga atggctttca
    10321 ccagtagctc gcgggcggcg agcagcttct gaaaatcgac cccggtgcgc acgcccatgc
    10381 tctcgaacat gaacaccagg tcctcggtga tcacgttgcc gctcgcaccg ggcgcgaagg
    10441 ggcagccgcc gaggccaccc atcgagctgt cgaaatggcg cacgccttcc tcgtaggcgg
    10501 cgagggcgtt ggcgagcccc aggccgcggg tgttgtgcag gtgcgcggct tcgagcttgg
    10561 cgccgatttc atctcggact tttctgaata accttcgaat ctgcgcggga ttcgcatatc
    10621 ccacggtgtc ggcgaggcac acgaagtcgc agaaatcggc gagcgccacc gcgatgcgca
    10681 ccacgtcgtc ttccggcacc tcgccctgca gcgtgcagcc gaacgcggtc gagacggcgc
    10741 cctctatttc gacgcccgga tgcgggtgct gcgcgcactt gcgcacttcc tcgaccatct
    10801 gctccggggt catgcgcacg ttcgattcgc tgtgcttgcg gctcgccgag accgggaacg
    10861 tcaccttgtg cgcgccggcc tcgagcgcgc gctggaagcc cttcaggttc ggggcgagcg
    10921 ccacgacctt cacgcctttc aatgtttttg caaacgcaac aatctcgccg gtgtccgcca
    10981 tggccgggat cagcttgggc ggcacgaacg agccgacttc gatctccttc agccccgccg
    11041 cggcgagcgc ggcgatccag gctttcttcg cctcggtcgg catcaggtgg tgcgcgttct
    11101 gcagcccgtc gcgcgggccg acttcgcaga tatggacgtt ttccatggcc gccattctac
    11161 ttgccgaggc gccgctcgaa ccgcccgacc gcgaccgcgg cgccgatgcc gacgagcgcc
    11221 agcgccgcca tcacttggag cgaggcgccc cagccgccgg cgcgcgacgc tacccaggcg
    11281 atcaggatcg ggatcgcgaa ctgggcgccg tgcgaggcct gcatgaccat gccgttggtg
    11341 gtgccgatgt gctgcgggct cttcgcgtgc accgtcgtgc ccgagaatac cgctgcggga
    11401 atcacgccgc cgagcagcga gaacgccatc acgcacgcga gccgcagcgt gtccggcagt
    11461 gcagtggaaa agaaaccaag cgcggtcgcg cccatggcga aggcgcctgc ccccatcacc
    11521 aaccagcgcg gcaggccggc gcgcagcagc aggccgccga gctggttccc ggggatgttg
    11581 atggcgacga acgctgccgt caccagcgcg gcggcggacg ggctcgcgcc gcgctcgtcg
    11641 acgacgaacg tcggcagcca ggtcatgagc gaggtccact ggccgacgta gcagatgaag
    11701 ccgacgcaga gcgcgaggct gccggggcgg gcgagcgatt cggcgagcag gcgcagcgag
    11761 ccgatgccgc tcccgaactg cgccggcggc accttgcgcg cgagcagcac cgcgcacaac
    11821 gcggcatagg ccgccagggc gacccaaagg ccgcgccagc cgaaccccga gagcacgagc
    11881 ggcgccacga ggagcgcgag cgtgccgccg gtcggcatgt acgagctcca gatcgaaaac
    11941 gccgtcgcgc ggtcctgcgg cagggtggcc gccgccagca gcggcgcggc ggccaccacg
    12001 aacaggagga agccgacgcc ttcgaggaag cgtgaggcca gcagcgtgcc gtagccgccg
    12061 gcgagcgcgc cgaccagccc gcccgccacc atcaccccga ggccggcgag ggccgcgcgc
    12121 ttcaccccga tgcgctcgcc gaacacgccg aagaacacgc cgaccgacgc gccgatcacg
    12181 taaagcatgg tctggatcag gcccgactcg acgagggtga gcccgaggtc ggcgcgcatc
    12241 gtgggcagcg ccggcggcac cttcgtcatg taggcgccgg cagcgagccc gccggcgaag
    12301 atgacccaaa ccgcgggcca attagtcaag ctgcggttac tggaatcttt agctcgacga
    12361 tcttcttcga ccactcggcg ggtccagtgg tgtgcaccga agtgccgctt gcatcgaccg
    12421 cgaccgtcac cggcatgtcc tccacctcga actcgtagat cgcctccatg ccgaggtcct
    12481 cgaaggcgag cacgcgcgac ttgcggatcg ccttcgagac gagataagca gcgccgccga
    12541 ccgccatcag gtaggccgcc ttgtgcttgc ggatcgcctc gatcgccgcc ggcccgcgct
    12601 cggccttgcc gaccatcgcg acgagtccgg tcttcgccag catcatctcg gtgaacttgt
    12661 ccatgcgcgt ggaagtcgtc gggccggccg ggcccaccac ctcgtcgcgc accggatcga
    12721 ccggaccgac gtagtagatg acgcggttgg tgaaatccac gggcagcggc ttcttctgtt
    12781 caagaaaatc ctggattcgc ttgtgggcgg catcgcgtcc ggtaagcaat tttccggaga
    12841 gaagcaggcg ctggccgggt ttccaggacg ccacttccgc ccgcgtcaga gtatccaaag
    12901 aaactctttt tgaagccgaa tcgggcttcc aggttacggc cggccaatcc gagagcttgg
    12961 gctcctcgaa ttccgccggg cccgagccgt cgagcacgaa gtgcgcgtgc cgcgtcgccg
    13021 cgcagttggg gatgatcgcg accggccggt tggcggcgtg cacgggatag gtgcggatct
    13081 tgacgtcgag gaccgtggag agtccgccca ggccttgcgc gccgatgccg agcgcattca
    13141 ccttgtcgta gagctcgacg cgcagctcct cgaccttgtt ccgggccccg cgcgctttca
    13201 gctcggccat gtcgatcggc tccatcagcg cttccttggc catcagcatg gccttctcgg
    13261 ccgtgccgcc gacgccgatg cccagcatcc ccggcgggca ccagcccgcg cccatggtcg
    13321 gcacggtctt cagcacccac tcggcgatcg agtcggaggg gttcagcatc gcgaatttcg
    13381 ccttcgcctc cgagccgccg cctttcgccg cgacgtcgac ctcgagcttg tcgccgggca
    13441 ccatttgcac gctgatcacc gccggggtgt tgtccttcgt gttcttgcgg ccgaaatccg
    13501 actcgtcgag caccgaggcg cgcagcttgt tgtccgggtc gaggtaggcg cggcgcacgc
    13561 cctcgttcac ggcggcttcc aggtcgtcga aatcgaaccg cacctgcatc ccgatcttca
    13621 ggaagacgtt gacgatcccc gtgtcctggc agatcgggcg gtggccctcg gcgcacatgc
    13681 gcgagttggt gaggatctgc gcgatcgcgt ccttggccgc ggcagactgc tcgcgctcgt
    13741 aggcgcggcc gagcgcccgg atgtagtcgg ccggatggta gtaggagatg aactgcagcg
    13801 cgtcggcgac gctctgcacg agatgcggac gcgtgatggt cgtcatgcgt gcttttgttc
    13861 gtgaggggcg cccacggcgg gcgtgatgcg atgcgcggcc gcgatcgcca gcgccgacac
    13921 caggaaagtc agatggatgc cgacctgcgc gacgatcagc ccgaggtaga ggggtagctg
    13981 cagccagcgg ctggcaaaga tgaaagcggg gagcgggcgc aaggcgcgcg cctcggtcat
    14041 tccgggggaa ttctgggcca tcttcgtttc gcttgaatgc gagggtgaaa ctgtagcatt
    14101 ctattcggac cgtccagagg agaagcgcat ggccacgtcg accatcgaat cggtgctgca
    14161 ggagaagcgg gtgtttccgc ctgccgccgc cattgtcaag caggcgaaca tctccgggat
    14221 ggaggcgtat cgcaagctcg tcgccgagtt cgagcgcgac ttccagggca cctggggccg
    14281 cctcgcgcgc gaaacgctgc actggtcgaa gcccttcacg aaggtgctgg acgaatccaa
    14341 ggcgccgttt ttccgctggt tccacgacgg cgagctgaac gcttcctaca actgcctcga
    14401 ccggcacctg aagacgcaac ccgacaagac ggcgatcatc ttcgaagccg atgacggcaa
    14461 ggtgacgagg atcacctacc aggagttgta caaggaagtc tgcaagctcg cgaacgcgct
    14521 caaggcgaaa ggcatcaagc agggcgatcg cgtgctgatc tatatgccga tgtcgatcca
    14581 ggcagtcgtc gccatgcagg catgcgcgcg catcgccgcc acgcattccg tggtgttcgg
    14641 cggcttctcg gcgaagagcg tccaggagcg catcatcgac gccggcgcca tcgccgtgat
    14701 cacggcggac ggccaattcc gcggcggccg cgagatcccc ctgaagcccg tggtcgacga
    14761 ggcgctcggc atgggcggct gcgaggggat caaaaatgtg atcgtgtacc agcgctcggg
    14821 cagcaaggtg ccgatgcagg ccgggcgcga cacctggtgg gacgatgcgg tgaaaggcca
    14881 ggctgatacc tgcgcgccga cgttcgtcaa cgccgagcat ccgctgttca tcctctatac
    14941 ctcgggctcg accggcaagc ccaagggcat ccagcattcc accggcggct atctgctgtg
    15001 gtgtgcgctc accatgaaat gggtgtttga cagcaagcct ggcgacgtct tctggtgcac
    15061 ggcggacgtc ggctgggtga ctgggcacag ctacatcgcc tacgggccgc tcgccgtggg
    15121 cgcgaccgaa gtggtgttcg aaggggttcc cgtctatccc gacgccggcc ggttctggaa
    15181 ggtcatccag gatcacaagg tcaacgtgtt ctacacggcg ccgaccgcga tccgctcgct
    15241 gatcaaggcc ggcggcgacc tgccgaagaa gtacgacctt tcgtcgctgc gcatcctcgg
    15301 cacggtgggc gagccgatca atccggaggc gtggatgtgg taccacgaga ccgtcggcgg
    15361 cggccgctgc ccgatcgtcg acacctggtg gcagaccgag accggcggcc acctgatctc
    15421 gccgttgccg ggcgccacgc cgaccaagcc gggctcggcc acgctgccgc tgcccgggat
    15481 cttcgccgac atcgtcgacg agaccggcca ttcggtgggc cgcggcaagg gcggcatcct
    15541 ggtgatcacg cgcccctggc cgtcgatgct gcgcacgatc tggggcgatc ccgagcgcta
    15601 caagaagacc tactggcccg acgatttcaa ggggaagctg tacctcgccg gcgatggcgc
    15661 gagcatggac gaggaagggt atttccgcat cgtgggacgc atcgacgacg tgctgaacgt
    15721 gtccggccat cgcctgggga cgatggaggt cgaatcggcg ctggtggcga actcgaagct
    15781 ggtcgctgaa gccgcggtgg tgggcaagcc cgacgagttg accggcgagg cgatctgcgc
    15841 cttcgtggtg ctgaaggggc cgcggccgaa cggcgcggag gcgaagagga tcgccgaaga
    15901 gctgcgccaa tgggtggcga aggaaatcgg gccgattgcg aaaccgaagg acatccgctt
    15961 cggcgacaac ctgccgaaga cgcgctccgg gaaaatcatg cggcgcctgt tgcgctcgat
    16021 cgcgaagggc gaggagatca agcaggacgt gtcgacgctc gagaacccgc agatcctcga
    16081 gcagttgaag cagtcaacgt gatcgaacgg cacaaggcgt actggcgcaa gaccctgacg
    16141 atgaccggca ggctgctgta cgccgccatc atcgcgttct acgcctggta cttgaggaag
    16201 cagaccgaac cctgattcgg cgggaggagg aggcatggag gaattcgacg tacgcgggca
    16261 gcgcctggcg gcgctgtgcc tgctcggcct gctgctcttc agctacccgg tgctcgcggt
    16321 gttcaacgtc caggccacgg tggccggcat cccggtgctg tacgcgtact tcttcgtcgc
    16381 ctggggggcg ctgatcgggc tggtcgccct cgtcatcgag cgcacgccat agcggagcga
    16441 aacggtgctc gagacctggg tcgtcattgt caccgcgttc gcttacctcg ggctgctgtt
    16501 cgcgatcgcg tactacgccg accagcgcgc ggacgcgggg cgctcggtga tcgcgagccc
    16561 ctacatctac agcctgtcgc tcgcggtgta cgcgaccgcg tggaccttct acggcagcgt
    16621 cgggcgcgcg gcggagaacg gggtcggctt cctgccgatc tacatcgggc cgacgctgat
    16681 gatcgcgctg tggtgggtgg tgatgcgcaa gatcctgcgc atcagcaagc aaaaccgcat
    16741 cacctcgctc gcggacttca tcgcctcgcg ctacggcaag agcgcgctgc ttggcggtgt
    16801 ggtcaccgtg atcgcggtga tcggcatcct gccctacatc tcgctgcagc tgaaggcggt
    16861 gtcggtcagc ttcaccacgg tcgcgggtgc ggtcccgggg ctcggcgaca ccgcgctctg
    16921 ggtggcgctg atcctggcgg cgttcaccat cgccttcggc acgcgccacc tcgacgccgc
    16981 cgagcaccac gaaggcatgg tggcggcgat cgccttcgag tcgctggtca agctgctcgc
    17041 atttctcgct gtgggcgcct gggtgagctg gggcctgtac aacggcttcg gcgacatctt
    17101 cgcgcgtgcc gcggccgagc cagagctgcg accgatgctc gcgccgttcg gcggcgtggg
    17161 cggcgactat tcgagctgga tctggctcac gctgctctcg atgctggcga tcatgttcct
    17221 gccgcgccag ttccaggtga cggtgatcga gaacaaggac gagagccacc tgaagaaggc
    17281 gatctggctg ttcccgctgt acatgctcgc gatcaatatc ttcgtgctgc cgatcgcgtt
    17341 cggcggcctg ctgcactttc cgagcggcag cgtggacgcc gactccttcg tgctcacctt
    17401 gcccctgtcg cagaagcagg agctgctcgc gctggtggtg ttcatcggcg gattgtcggc
    17461 ggccaccggg atggtgatcg tcgagaccat cgcgctctcg accatggtgt gcaacgacct
    17521 ggtgatgccg gtgctgctgc gcatgcgcgc gctgcgcctg aaccagcgcc gcgaccttac
    17581 cgggctgctg ctcggcatcc ggcgcggcgc gatcgtgctg atcctgctgc tcggctacct
    17641 ttacttcaag gtcgcgggcg aagcctacgc gctcgtctcc atcggcctga tctcgtttgc
    17701 cgcggtggcg cagttcgcgc ccgcggcgct gggcggcatt ttctggaagg gcggcacgcg
    17761 gcgcggcgcg ctggcggggc tgggcgccgg cttcgcggtc tggttttata cgctgctgct
    17821 gccggcgttt gcgcgctcgg actggctgcc gatcggcttt ctcgagcacg ggccgttcgg
    17881 cttcgagctg ctcaagccgg tggcgctgtt cggcgtctcc ggcctcgacc agatcacgca
    17941 cgccatgatc tggagcatgg tggcgaacgt cggcgcctac gtcggcgtgt cgctcatgcg
    18001 ctcgccgagc gcggaggagc accgccaggc gacgctgttc gtcgacgtgt tcaagcggcc
    18061 agccggcgcg cgcttgcggc gcggaaccgc gtcggaagcg gagctgttca acctgctcgc
    18121 gcgcttcgtc ggcctgcgcg cggcgagcga cgcattcgcc gagcatgccc gccgtggcgc
    18181 cgacgcggat ctggtgcaat tcgtcgagcg ccagctcgcg ggcgtgatcg gcgccgcctc
    18241 ggcgcacatc atgctcgccg cggtggtgaa ggaggaggcg ctgacgctgg acgaggtgcg
    18301 cgcggtgctc gacgacgcgt cgcagatcgt ggtgtacagc caccgcctgg agcagaagtc
    18361 gcaggagctc gagcgcgcga cgcaggagct gcgcgcggcg aacgagcgcc tcaccgagct
    18421 cgaccgcatg aaggacgagt tcgtctccac cgtgtcgcac gagctgcgca cgccgctcac
    18481 ctcgatccgc tcgttcaccg agatcctgca cgacaacccg cagctgccgg ccgccgagcg
    18541 cagccgcttc ctcggcatcg tgatcaagga gaccgcgcgg ctcagccggc tgatcggcca
    18601 ggtgctcgac gtctcgcgcc tggaatccgg ccgcgtcgag tggcacgagt cgctgctcga
    18661 catgcgcgag gtgatcgagg acaccgcggc gtcgaccagc cagctgttcc gcgagcgcaa
    18721 tgtgcgcctc gagcgcaacc tgccgggccg cgtgcccaag gtgagggccg acctcgaccg
    18781 catcgtccag gtggtggtga acctgctgtc caacgcggtc aagttcatcg agccggggcg
    18841 cggtcgggta gagatcgcgc tggccgagga ggcgggcttc ctgcgggtcg acgtgcgcga
    18901 caacggcccc ggcatcagcc ccgagcacca ggagatgatc ttcgagagat tccgccaggc
    18961 cggcgatccc ctgaccggca agccgcaggg cacgggcctg ggcctgtaca tcagccgccg
    19021 catcatcgag cactcgggcg gccgcctgtg ggtgagcagc cggccggggg agggcgcatg
    19081 cttctccttc acgctgccgc tggtggccga agcgagcctg ggcagggccg cgtgaccgcg
    19141 aaacccgctc ccaccgccta cgtggtcgac gacgacgaat cgatccgctc cctctggcaa
    19201 tggttgctgg gctcgaacgg catcgcggtg cagaccttcg ccaccgccac ggcgttcatc
    19261 gacgcctatc gccggggcga cgccggctgc ctggtgctcg acgtgcggct gcccggcatg
    19321 agcgggccgg agctgcagga ccacctgcgg cgcgaggcga tcgagattcc catcgtcttc
    19381 gtcagcgccc acggcgacgt gcccaccgca gtcaatgcga tcaagggcgg cgcggtggat
    19441 ttcatccaga agccgttcga ctatcgcgag gcgctggcga tcgtgcggcg cgcactcgag
    19501 cgcgacgcgc aggtccgcga gcggcgtgcc agacaaacgg agctcgccgg gcgttttgcc
    19561 gcactgaccg cacgcgagcg cgaggtgctg catcgcatca tcgaaggcaa gtcgaacaag
    19621 gtcatcgccg aagagctcga catcagcgtg cgcaccgtgg agttccatcg cgccaacgtc
    19681 atggagaaga ccggggcgga atcggtaggc gcgctcatcc agcaaatggc gcttcacagc
    19741 gggctggagt agccgtcgtt tggtgcggtg ccgcaaaacc tagtagtact acgaggggca
    19801 gaggaccagc agttccacta ggatgccgtg caggatttcc aaagggggag gcctgattgg
    19861 aactgactga gaagcatcga gagtactggc ggaagaacct cgtcattacc gccatcctgc
    19921 tggccgtctg gttcgtggtc accttcgtcg aaggctggta cgcaagagag ctgaacaaca
    19981 tcagcttcct cggctttccg ctcgggttct acatgtcggc gcagggctcg ctgatcatct
    20041 atgtggtgat catcggcatc tatgcgcgtt acatgaacaa cctggacaag gtgtacgacg
    20101 tgcacgaggg ggaagacgaa tgagcgccgt ttacagccac aagagcttca attcgcagct
    20161 caagaagtac tactcggtct ataccggcgg cttcatcgcc ttcgtcatcg cgctcgccat
    20221 cgccgagcag atcggcatgt cgcgcgcctg gatcggctac tggttcctgt tcgccaccat
    20281 cgcgctgtac gccgcgatcg gaatcatgag ccgcaccgtt gacgccgccg agtactacgt
    20341 ggcaggccgg cgtgtgccgg cgttcttcaa cggcatggcg accggcgccg actggatgag
    20401 cgcggcctcc ttcatcggca tggccggcac gctctacctc tccggctacg acggcctgtc
    20461 gttcgtcatg ggttggaccg gcggctacgt gctggtggcg ctgttccttg cgccttacct
    20521 gcgcaagttc ggccagttca ccatcccgga cttcctcggc gcgcgctacg gcggcaacat
    20581 cgtgcgctcg atcggcatct tcgccgcgat cctgtgctcg ttcacctacg tggtggcgca
    20641 gatctacggc gtgggcatca ttaccagcaa gttcaccggg ctcgagttcg gcatcggcgt
    20701 gttcgtcggc ctggccggca tcctggtgtg ctcgttcctc ggcggcatgc gcgcggtcac
    20761 ctggacgcag gtcgcgcagt acatcattct gatcatcgcc tacctcacgc cggtggtctg
    20821 gctgggcgtg aagcacaccg gcatcccgat cccgcagttc gcctacggct acacgctgca
    20881 gaaggtcggc gacctggaga agaagatcac cgccgatccg aaggaagtgg aggcgcgcgg
    20941 gctggcgaag gcggccgcag ccgaagccag cgcggcgctg aaagatcccg tcaaggcggc
    21001 cgccgaacgc cgcgcggcgc tggagcagcg cctcgccaag gccaaggcgg acaacgcgcc
    21061 ggaccatgtc cagaagtcca tccagcaggc gctcagcaac acgcctaccg atccggccaa
    21121 ggcgcaggag tactggtcgg cgcggcgcaa cctgggcgct aacgccaatc cgccgcgcaa
    21181 gcatgcagat ccgttcccgg gagcaaccga ggaggcgcgc cacaccgcca agctcaactt
    21241 cctcggcatc gtgttctgca tgatgttcgg caccgcggcg ctgccgcaca tcctgatgcg
    21301 ctactacacc acgcccagcg tgaggcaggc gcgcacctcg gtgttctggt cgctgttctt
    21361 catcttc
//
LOCUS       DATBSZ010000018        16093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_616932, whole genome shotgun sequence.
ACCESSION   DATBSZ010000018 DATBSZ010000000
VERSION     DATBSZ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16093
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616932"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VIG70_01045"
     CDS             <1..1020
                     /locus_tag="VIG70_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8249162.1"
                     /translation="TLQADPQIGRMDTDLQLDLPQLILEPDRVRAAGLNLTSGDVALA
                     VSMLTGGIDIAKYNDYPGDGERYDIRVKAKDGEFQQQADLSKIYLRNRQGQLVRLDQV
                     ARFREALGPAVISRFDLQYSATFYATPTVSLGAAIEKVRAAAQSLPIGYRVVFVGEAE
                     QLEKTATATQFTFILALALLFMVLASQFNSFLQPLYIMLAVPLGIVGGIAALWLSGSS
                     LNIYSGIGLVLLIGLVAKNSILLVDLTNQRRDQGMSVDDALRDACPIRMRPVVMTSLT
                     IILALTPAAIGVGAGAETNKPLSLAVIGGMITSTLLTLVVVPAGYSVVEGWRERRRAG
                     KPKLA"
     gene            962..1801
                     /locus_tag="VIG70_01050"
                     /pseudo
     CDS             962..1801
                     /locus_tag="VIG70_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004542360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            1851..2330
                     /gene="mug"
                     /locus_tag="VIG70_01055"
     CDS             1851..2330
                     /gene="mug"
                     /locus_tag="VIG70_01055"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF007570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEY8249163.1"
                     /translation="MIAPGLKVLFVGINPGLYSAATGHHFARPGNRFWPALKQAGFTE
                     RQLHPSQQEELLKDGYGITNLVNRGTATADELAPWEFVKGRRRLAAKVRRYRPATVAF
                     LGVGAYCHAFSCKEAKVGPQSDRFEAARVWLLPNPSGLNANYQLPELVKLFRALKRS"
     gene            2334..2528
                     /locus_tag="VIG70_01060"
     CDS             2334..2528
                     /locus_tag="VIG70_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249164.1"
                     /translation="MATKLDKFLKREIAIEGNPYMLTIGPEGLKLVPKGKRNGLELSW
                     KALVSGDAALATALNASLQK"
     gene            2509..3117
                     /locus_tag="VIG70_01065"
     CDS             2509..3117
                     /locus_tag="VIG70_01065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY8249165.1"
                     /translation="MPPSRSRDLNGAAPDNCPVALLLIDWINDLEFEGGERLFPQALA
                     AARAAAGLRRRAKQAGVPVIYCNDNFGKWRSDFRSLLEHCLKDEVRGRPIAELLAPDD
                     EDYFVLKPKHSGFHSTSLEVLLAHLGARTLILTGIAGNFCVLFTAHDAYMRDYSLVVP
                     RDCLASEDEADNRHALEHMAKACKADTAASSGIDFANLRRAA"
     gene            complement(3128..3901)
                     /locus_tag="VIG70_01070"
     CDS             complement(3128..3901)
                     /locus_tag="VIG70_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY8249166.1"
                     /translation="MTEPVSEHDLQAYADGELPEDRRAAVAAWLAAHPEDAERIESYR
                     ALAQELRSTYDGVLHEPVPERLEQALRPLRSRRFALVAMWVVLGAALGALAGWQLHST
                     QTSRVVAADGAMVLARRAAVAHATYSPEVRHPVEVGADQETHLVAWLSKRLGTQLRAP
                     KLENVGYSLVGGRLLPGEQGPVAHFMYQCNRGTRVTLYVRSDMGQNRSTAFRYAHEGT
                     VGVFYWVDSKLGYAISSADISKEDLLNVANAAYQQLNPP"
     gene            complement(3898..4398)
                     /locus_tag="VIG70_01075"
     CDS             complement(3898..4398)
                     /locus_tag="VIG70_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8249167.1"
                     /translation="MNEAERLVELIPRLRRYARALVGDRATADDLVQDTLERAWAKLH
                     LYRRGTDLRAWLFTVMHNVHVNRVRAARATDTLEDEMPELAQRAPQGDALLVRDLDRG
                     ITRLPAEQRAVLLLVTLEEMSYEQVARTLGIPIGTVMSRLSRAREKLRAMMLGQPVAA
                     KLKVVK"
     gene            complement(4434..5291)
                     /locus_tag="VIG70_01080"
     CDS             complement(4434..5291)
                     /locus_tag="VIG70_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8249168.1"
                     /translation="MFQASPATAGLLLFLAATLCFAQGDAKRGEYLSKAGGCLGCHTL
                     EAKDAKDKPVPYAGGRALKTPFGTFHGPNITPHPEAGIGRWSEADFIRAMREGRRPDG
                     SNYFPAFPYPSFTQIADADLRDLWAYLRSLPQSSRQSEPHDLGVLYRWRFLVSGWKWL
                     FFTPGPLAPEAGKSAQLNRGAYLVRALGHCGECHTPRNFLGGPKKDRFLAGAKDVAPN
                     LTPTRLGKKGDGELKEFLRTAMTPDGDVAAESMAEVVTNTTSQLTPQDLDALVAYLRA
                     LPPLPEAPK"
     gene            complement(5299..5760)
                     /locus_tag="VIG70_01085"
     CDS             complement(5299..5760)
                     /locus_tag="VIG70_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8249169.1"
                     /translation="MKKTLLAAGVAIALGGVAGVALAQVKPDVLVKQRQSAMTLIAKY
                     WGPIAGMASGKVSPYNADVVSRNATYLENLAQMPWDGFHENTKGEKNTKALPEIWSQK
                     AKFDELAQRLQTETAKLGEIARKKDEAGVKQQFAAVGKVCGACHESFREKQ"
     gene            complement(5831..6412)
                     /locus_tag="VIG70_01090"
     CDS             complement(5831..6412)
                     /locus_tag="VIG70_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8249170.1"
                     /translation="MSAAPVHAQDLAKAQNTANQVCASCHAGDGNSTAPANPKIAGQF
                     PEYLHKQLADFKPQGGKKPARESPIMMGMVANLSDADMKGLAAFYAGQKLKPAAAADK
                     NLAALGQKLWRGGNTATGVPACAGCHGPAGAGMPAQYPRLAGQYAEYVAAQLKAFKEG
                     GRANDPNGMMRGVTARMTEREIRAVAEYAAGLR"
     gene            6489..7064
                     /gene="yihA"
                     /locus_tag="VIG70_01095"
     CDS             6489..7064
                     /gene="yihA"
                     /locus_tag="VIG70_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY8249171.1"
                     /translation="MFSQAQFLAAAGRPGDIPPAGPPEVAFAGRSNVGKSSAINALLG
                     RKRLAYTSKTPGRTQTINFYELGSVARLVDLPGYGFARAPHALRRQWKDLVDAYLQSR
                     PTLAGIVVIMDARHPFTALDEQLLEWLGDSRKLVLLSKADKLSRTEQAALMKSYGDAL
                     LFSSTTRQGVEECCNLLESWLQPAAEIKSPR"
     gene            7207..8265
                     /gene="hemB"
                     /locus_tag="VIG70_01100"
     CDS             7207..8265
                     /gene="hemB"
                     /locus_tag="VIG70_01100"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY8249172.1"
                     /translation="MNIYGNFPALRMRRMRRDEFSRRLMRETTLTTGDLIYPVFVLDG
                     KARTEKVASMPGVERMTVDKLLPLAEQCLKLGIPVLALFPVVESRLKTADGREAFNAK
                     GLVPRAAAALKKRFPELGLMTDVALDPYTSHGQDGLIDENGYILNDATLDVLVKQALA
                     QAAAGVDIVAPSDMMDGRIGRIRAALEKAGHIHTKIMAYSAKYASGFYGPFRDAVGSA
                     KALGKADKRTYQMDPANSDEALWEVGLDLAEGADMVMVKPGLPYLDVLRRVKDEFKAP
                     TYVYQVSGEYSMLRAAIANGWLPESCVMESLIAFKRAGADGILTYFAFEAAKVLQLPE
                     KGSSGSARRSGTRSRAAP"
     gene            complement(8186..9931)
                     /gene="dsbD"
                     /locus_tag="VIG70_01105"
     CDS             complement(8186..9931)
                     /gene="dsbD"
                     /locus_tag="VIG70_01105"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEY8249173.1"
                     /translation="MNRFLIGLLLWILAAPGSAQLFQKSEELLEPEKAFRFSARAAAG
                     EVEVSFAIADGYYMYRDKFRFTAEGNPAVRLGAAQFPAGARHKDEFFGEVETYRRQVR
                     IRIPAQGEGRFDLKVVSQGCADVGVCYVPMESQASLRLVAGDAGGAVMSPGPRQVDAE
                     RRWSIFASDMDIAQLFQGNAALVLASFFGFGLLLSFTPCVLPMIPILGGIVAGEGARI
                     DKSRALLLSGTYVLSMALTYALAGIAAAYVGSLIAAYLQNVWVLGAFAAIFVLLALSM
                     FGVYELRLPHGVQQRLHTAHGKLKGGRLVPVAAMGVLSAVIVSPCVSAPLAGALLEIS
                     RSGDMVLGGSALFAMALGMGVPLIAVGVSEGALLPRAGAWMATVKNFFGLMLLAVAVW
                     ILVPVLPPLASMLAWSLLAFGAASLLRRSRIAAAVPLIAGAALLVGALAGSRDPLRPL
                     AVFTAGTPHSQPVAWTRVASLTELQTRLKAPGKPVMLDFYADWCVSCKEMESFTFSDP
                     KVRSQLDRMLLLQVDVTANNENDKALLKRFSLFGPPGIVFFDAEGREIKGLRVIGYQN
                     AERFLKTLSLATAAL"
     gene            complement(9928..10251)
                     /gene="cutA"
                     /locus_tag="VIG70_01110"
     CDS             complement(9928..10251)
                     /gene="cutA"
                     /locus_tag="VIG70_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEY8249174.1"
                     /translation="MQSTKLLVFTNVPDRAVAEKLADMLVERRLAACVNILAPCRSVY
                     RWKDAVQHDEEHPMLIKTTQERYAALEQALRAGHPYELPEIIAVPIERGLPAYLDWVA
                     AETKA"
     gene            10267..11301
                     /locus_tag="VIG70_01115"
     CDS             10267..11301
                     /locus_tag="VIG70_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEY8249175.1"
                     /translation="MHKVTVQPSGQAFEVQEGESVLTAALRQGVMLPYGCKNGACGSC
                     KGKIVAGAVDYGHYHARVLTEAERAHGKALFCQAKPLGELVIECRTIGAAKDIAVRLL
                     PCRVQKLEKVADDVMIVRLKLPANERLQFLSGQYIDFLLKGGERRSFSMANAPHADEL
                     IELHIRQVAGGTFTDHVFGKMKERDILRLEGPLGSFFLREDSAKPIVFVASGTGFAPI
                     KSIIESAFHRNVTRPMVLYWGARRPKDLYLSSIPETWAREQPNFRYVPVISEARPEDQ
                     WSGRSGFVHRAVMQDFPDLSTHQVYACGVPVMVDSARKDFTTLCQLPEDEFYADSFTT
                     QADLASDELR"
     gene            complement(11315..12016)
                     /locus_tag="VIG70_01120"
     CDS             complement(11315..12016)
                     /locus_tag="VIG70_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249176.1"
                     /translation="MLLEVKDLEVHYGGIRAVKGIDLEVKEGELVCLIGANGAGKTTT
                     LKAICRLIPSRSKKLTYEGSDISAAKVHELPRRGLVMVPEGRGIFPQLTVEENLAMGA
                     YARGSTDTEKQYATFPRLKERRLQVAGTLSGGEQQMLAIARALMSDPKLLLLDEPSMG
                     LAPMMVARIFEVVRDIASRGVTILLVEQNARLALELAHRGYVMESGVISLSGRSADLL
                     NDSRVKEAYLGEDAA"
     gene            complement(12006..12773)
                     /locus_tag="VIG70_01125"
     CDS             complement(12006..12773)
                     /locus_tag="VIG70_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249177.1"
                     /translation="MNLLTADGVSKRFGGVQALNAVSFSIARGQIYGLIGPNGAGKTT
                     LFNVLTGIYPIDAGAFTFDGSALAGLKAHQVAAAGIGRTFQNIRLFVNLSALENVMIG
                     RHARTRAGVVGAVLRNKRTLKEEREIEKRSLELLDYVGIRARANDAAGSLPYGDQRRL
                     EIARALAMEPKLLALDEPAAGMNPAERIALGKLIRRIRDDGVTVLLIEHDVRLVMNVC
                     DRVLVLDYGERIAEGTPAEVQKDPKVIEAYLGAPHAA"
     gene            complement(12770..13813)
                     /locus_tag="VIG70_01130"
     CDS             complement(12770..13813)
                     /locus_tag="VIG70_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249178.1"
                     /translation="MRQYFDTKGKPWLAWVGIFLIALSLAVLPFAIAQAGTTWVRITN
                     YAILYILLALGLNIVVGFAGLLDLGYIAFYAVGAYVYALLASPQLGLHLPFWVILPLG
                     AAVAALFGVMLGAPTLKLRGDYLAIVTLGFGEIIRIFLNNLSRPVNITNGPQGITGID
                     PFVLGGFRFDSPIKYYYLLLAVALGIILVNLRLQNSRIGRAWEAIREDETAARAMGID
                     TRNLKLLAFAMGASFGGIAGGMFSAIQAFISPESFVLVESIMVVSMVVLGGMGNIWGV
                     ILGALLLSFVPEILRWTVTPVQEALFGRMLIEPEVIRMLLFGLALVLVMLYRPAGLLP
                     SAVRKRELEERAA"
     gene            complement(13813..14787)
                     /locus_tag="VIG70_01135"
     CDS             complement(13813..14787)
                     /locus_tag="VIG70_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8249179.1"
                     /translation="MDTFLQQLVNGLTAGSVYAVVALGYTMVYGIIQLINFAHGEVVM
                     IGAMVAFTIINLLAPTGLPALAIVLIGTGCAVPICMLVGYSMERLAYRPLRHAPRLAP
                     LITAIGVSIILQHLAMLVWSRNVLAFPQIIKSQTYFIGGAGITTVQIAILAICVTLMA
                     SLAILVYRTPLGTAMRATAQNAQVAGLMGIDINRVIAITFIIGAGLGAVAGVLFGTYY
                     GIAQYTMGSVLGLKAFSAAVLGGIGNIPGAMLGGVLLGLVEALGAGYIGDFTDLCQWG
                     LPLQACEGGGHFILMGSNYQDVFAFIVLILVLVFRPQGLLGERVGDRA"
     gene            complement(14859..16073)
                     /locus_tag="VIG70_01140"
     CDS             complement(14859..16073)
                     /locus_tag="VIG70_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8249180.1"
                     /translation="MNKISSITLLAAAVALIGCGDQKPAATADKGVAPAAAGGLEVKI
                     GHVGPLTGGIAHLGKDNENGARLAVEEANAAGIKIDGKAVKFTFVAEDDQADPKVGTT
                     VAQKMVDAKVAGVVGHLNSGTSIPASPIYNGAGIPVISGSATNPKLTEQGFKTQFRVV
                     GRDDQQGPAIASYLATNNKPQTVAVIDDATAYGEGIANEVEKTLKAAKINVLPREKGT
                     DKTTDWKAVLTKLRGRNPDAVFYGGMDATGGPLLKQGRELGIKAVFSFGDGACTDKMK
                     ELAGDAAEGLLCSQAGIPVEAADKKFLEAYKKKFNADPILYSPFTYDAANMLIQAMQK
                     ANSSDPKKYLPELQKLDFKGVTGKIAFDSKGDRKDAEMTIFTMKGGKLLPVAVITGGK
                     SQSFDAFVKGMK"
BASE COUNT         2760 a         5364 c         5309 g         2660 t
ORIGIN      
        1 acgctgcagg ccgacccgca gatcggccgc atggacaccg acctgcagct cgacctgccg
       61 cagctgatcc tcgagcccga tcgcgtgcgc gcggcgggcc tcaacctcac ctcgggcgac
      121 gtcgcgctcg cggtgagcat gctcaccggc ggcatcgaca tcgcgaagta caacgactat
      181 ccgggcgacg gcgagcgcta cgacatccgc gtgaaggcga aggacggcga gttccagcag
      241 caggcggatc tctcgaaaat ctacctgcgc aaccgccagg gccagctggt gcgcctcgac
      301 caggtggcgc gcttccgcga agcgctcggc ccggcggtga tctcgcgctt cgacctgcag
      361 tactcggcga ccttctacgc cacgccgacg gtttcgctcg gcgcggcgat cgaaaaggtg
      421 cgcgccgcag cgcagagcct gccgatcggg taccgcgtgg tgttcgtcgg cgaggccgag
      481 cagctcgaga agaccgctac ggcgacgcag ttcaccttca tcctggcgct cgcgctgctc
      541 ttcatggtgc tcgccagcca gttcaacagc ttcctgcagc cgctctacat catgctcgcg
      601 gtgccccttg gcatcgtcgg cggcatcgcc gcgctctggc tttcgggcag ctcgctcaac
      661 atctactcgg gcatcggcct cgtgctcctc atcgggctgg tcgcgaagaa ctcgattctg
      721 ctcgtcgacc tgaccaacca gcgccgcgac cagggcatga gcgtcgacga cgccctgcgc
      781 gacgcctgcc cgatccgcat gcggccggtc gtcatgacct cgctcaccat catcctcgcg
      841 ctcaccccgg cggcgatcgg cgtcggcgcc ggagccgaga ccaacaagcc gctgtccctc
      901 gcggtgatcg gcggcatgat tacctccacc ctccttaccc tcgtggtggt gcccgcgggc
      961 tattccgtgg tcgaaggctg gcgcgagcgg cgccgcgcag gaaagcccaa gcttgcctga
     1021 cgcccgcagc ctggcggttt tctgcgactt cgagaacatc gcgctcggcg tacgcgaggc
     1081 gcggtactcg cagttcgaca tggagaaggt gctcgagcgg ctgctcgcca agggctcgat
     1141 cgtggtgaag aaggcctact gcgactggga gcgctacaag gagttcaagg ccaccatgca
     1201 cgcggcctcg ttcgagctga tcgagatccc gcacgtgcgc cagtcgggca agaactcggc
     1261 cgacatccgc atggtggtcg acgcgctcga cctctgctac accaaatccc acgtggacac
     1321 cttcgtcatc gtcagcggcg actccgactt ctcgccgctc gtgtccaagc tgcgcgagaa
     1381 cgcgaagacc gtgatcggcg tcggcgtcaa gaactcgagc tcggacctcc tcatcaacaa
     1441 ctgcgacgag ttcatctact acgacgacct ggtgcgcgcc gacgagcaga agcgccgcgc
     1501 ggcgaaaaag cggcgcgagg cggcgcccgc gggcgccggc agcgacaagg gcaacggcga
     1561 caagaagcag gaggccttcg acctgatgct cggcacgctc gacgcgctga tggccgagcg
     1621 cgacgccgat gagcgcatct ggggctcgat ggtgaagcag gcgctgaaac gccgcaaccc
     1681 gggcttcaac gaatcgtact atggcttccg tgccttctcc gacctcctcg aggaagccga
     1741 aaagaaaggc ctcatcacgc tcgaagccga cgagaaatcc ggcggctacg tggtcaagcc
     1801 ggcctagcgc cgcggatctc gccgcggcgc gaggcaaggg catccgagac gtgatcgcgc
     1861 ccggcctgaa ggtcctgttc gtcggcatca atcccggcct gtactcggcg gcaaccggcc
     1921 accacttcgc gcgtcccggc aaccgtttct ggccggcgct gaagcaggcc gggttcaccg
     1981 agcgccagct ccacccatca caacaggaag aactgctgaa ggacggctat ggaattacca
     2041 acctcgtgaa ccggggtact gccacggccg acgagctcgc gccgtgggaa ttcgtgaagg
     2101 ggcgacgcag gctcgcggcc aaagtgagac gctaccgccc cgcgacggtc gcttttctgg
     2161 gggtgggcgc ctattgccac gccttttcgt gcaaagaagc caaggttggg ccccagtccg
     2221 accgcttcga ggccgcgcgg gtatggttgc tgcccaatcc gagcggactg aacgcgaact
     2281 accagctgcc cgagctcgtc aaactgtttc gcgcccttaa gcggagttga accatggcca
     2341 ccaagctcga caagttcctg aagcgggaaa tcgcaatcga gggtaacccc tacatgctga
     2401 cgatcgggcc ggagggcctg aaactcgtgc cgaaaggcaa gcgcaacggg ctcgagcttt
     2461 cgtggaaagc gctcgtcagc ggcgacgccg ccctcgccac cgcgctgaat gcctccctcc
     2521 agaagtaggg atctgaacgg cgccgcgccg gacaactgcc cggtagcgct gctgctcatc
     2581 gactggatca acgacctcga gttcgaaggg ggcgagcggc tctttccgca agccctggcg
     2641 gccgcgaggg ctgccgccgg gctgcgtcgg cgcgcgaagc aggccggcgt gccggtaatc
     2701 tactgcaacg acaacttcgg caagtggcgc tcggatttcc gctcgctgct cgagcattgc
     2761 ctgaaggacg aggtgcgcgg gcggccgatc gccgagctgc tcgcgccgga tgacgaggat
     2821 tacttcgtcc tcaagcctaa gcactccggc ttccactcca cctcgctcga ggtcctgctc
     2881 gcccacctcg gcgcaaggac cctgatcctc accggtatcg ccgggaactt ctgcgtgctc
     2941 ttcaccgcgc acgacgcgta catgcgcgac tacagcctcg tggtgccgcg cgattgcctg
     3001 gcctcggaag acgaggctga taaccgccac gcgcttgagc acatggcgaa ggcgtgcaaa
     3061 gccgataccg cagcgtcgtc cggcatcgat ttcgcgaacc tgagaagagc ggcctagccg
     3121 gggtggatca gggcggattg agctgctggt aggcggcgtt ggcgacgttc agcaggtctt
     3181 ccttgctgat gtccgccgaa gatatcgcgt agccgagctt gctgtcgacc cagtagaaca
     3241 cgccgacggt gccctcatgc gcgtagcgga aggcggtgga gcggttctgt cccatgtcgc
     3301 tgcgcacgta gagcgtgacg cgcgtgccgc gattgcactg gtacatgaag tgcgccaccg
     3361 gcccctgctc gcccgggagc agccggccgc cgacgaggct gtagccgacg ttttcgagct
     3421 tcggtgcgcg cagctgcgtg cccaggcgct ttgaaagcca cgcgacaagg tgcgtttcct
     3481 gatcggcgcc cacttcgacc gggtggcgga cctccggaga gtaggtggcg tgcgcgactg
     3541 ccgcgcgccg cgccaggacc atcgccccgt cggccgcaac gacgcgggag gtctgcgtcg
     3601 agtgaagctg ccaaccggcg agcgcgccga gcgccgcgcc gagaaccacc cacatcgcga
     3661 ccagcgcgaa gcgccgcgag cgcagcgggc gcagcgcctg ctccagcctt tcgggcacgg
     3721 gctcgtggag cacgccgtcg tacgtgctgc gcagctcctg ggcgagcgcg cggtagctct
     3781 cgatgcgctc ggcgtcctcg ggatgcgcgg caagccaggc cgctacggcg gcgcggcgat
     3841 cctccggcag ctcgccatcg gcgtaagcct gaagatcgtg ctcgctgacc ggctcggtca
     3901 tttcaccacc ttcaacttcg ccgccacggg ctgcccgagc atcatcgcgc gcagcttttc
     3961 gcgcgcgcgc gacaggcgcg acatcacggt gccgatcgga atcccgagcg tgcgcgccac
     4021 ctgctcatag ctcatttcct cgagcgtgac gagcagcagc accgcgcgct gctccgcggg
     4081 caggcgcgtg atgccgcggt cgaggtcgcg caccagcagc gcgtcgccct gcggcgcgcg
     4141 ctgcgcgagc tcgggcatct cgtcctcgag cgtatcggtg gcgcgcgcgg cgcgcacccg
     4201 gttgacatgt acgttgtgca tcaccgtgaa aagccaagcc cgcaggtccg tgccgcggcg
     4261 atagaggtgc agcttcgccc aagctcgctc caacgtgtcc tgcaccaagt cgtcggccgt
     4321 ggcacggtcg cctaccagcg cgcgcgcgta ccgccgcagc cgcgggatga gctcaaccag
     4381 gcgctcggct tcattcatag agggaacaag cctcccaaga cgcctattcc acgctatttg
     4441 ggtgcttcag gcaacggcgg gagggcgcgc aggtaggcga ccagggcatc gaggtcctga
     4501 ggcgtgagct ggctggtggt attagtcacg acctcggcca tcgactccgc cgccacgtcg
     4561 ccatcggggg tcatggcggt gcgcaggaac tccttcagct cgccatcacc cttcttgccg
     4621 agacgcgttg gcgtcagatt gggcgcgaca tctttcgccc cggcgagaaa gcggtctttc
     4681 ttcgggccgc cgagaaaatt gcgcggcgtg tggcattcac cgcagtggcc aagcgctcgc
     4741 acgagatacg cgccacgatt gagctgcgcg ctctttcccg cttccggcgc gagcggcccg
     4801 ggcgtgaaga acaaccactt ccagccgctg acgagaaagc gccagcggta gaggacaccg
     4861 aggtcatgcg gctcgctttg ccggctgctc tgcggcagcg agcgcaaata cgcccacagg
     4921 tcgcgcaggt cggcgtcagc gatctgagtg aacgagggat aaggaaaggc cgggaagtaa
     4981 ttcgatccgt ccggacgccg cccctcgcgc atcgcgcgga tgaagtcggc ttccgaccag
     5041 cggccgatgc ccgcctcggg atgcggcgtg atgttcggac cgtggaaagt gccgaacggc
     5101 gtcttcaggg cgcgcccgcc ggcgtacggg accggcttgt ccttcgcgtc cttcgcttcg
     5161 agcgtgtggc aaccgaggca accgccggcc ttggaaaggt attcgccgcg cttcgcgtcg
     5221 ccttgcgcga agcaaagcgt agccgcaaga aaaagcaaaa gccccgcggt tgcggggctt
     5281 gcctggaaca ccgttcgcct actgcttctc gcggaagctc tcgtggcagg cgccacagac
     5341 cttgccgacc gcggcgaact gctgcttcac gccggcctcg tcctttttgc gcgcgatctc
     5401 gcccagcttg gcggtctcgg tctgcagccg ttgcgcgagc tcgtcgaact tggccttttg
     5461 cgaccagatc tccggcaatg ccttggtgtt cttctcgccc ttggtgttct cgtggaaccc
     5521 gtcccaaggc atttgcgcca ggttctcgag gtacgtggcg ttgcgcgaaa cgacgtcggc
     5581 gttgtacgga gagactttcc ccgacgccat gccggcgatc gggccccagt acttggcgat
     5641 cagggtcatg gcggactggc gctgcttcac caggacgtcg ggcttgacct gcgcaagcgc
     5701 gactccggca acgccgccga gggcgatcgc aacgccggct gccagaaggg tcttcttcat
     5761 tgggtcgtct cctcgtatcg cggcaagcgc cgcactattg aaaaacacct atggcaacgc
     5821 aagtattccg tcagcgcagc ccggcggcgt actcggctac cgcccggatc tcgcgttccg
     5881 tcatgcgcgc ggtgacgccc cgcatcatgc cattggggtc gttggcccgc ccgccttctt
     5941 tgaaagcctt gagctgcgcg gcgacgtatt cggcgtactg ccccgcgagg cgcgggtact
     6001 gggccggcat gccggccccg gcggggccat ggcagcctgc gcaggccggg acgccggtcg
     6061 cggtattgcc gccgcgccag agcttctggc cgagggccgc gaggttcttg tcggccgcgg
     6121 ccgcgggctt gagcttctgg ccggcgtaga acgcggccag ccccttcatg tcggcgtcgg
     6181 agagattcgc caccatgccc atcatgatgg ggctttcgcg cgccggcttc ttgccgccct
     6241 gcggcttgaa gtcggcgagc tgcttgtgca agtactcggg gaactggccg gcgatcttgg
     6301 gattggcggg cgcggtgcta ttgccgtcgc ccgcgtggca cgaagcgcag acctggtttg
     6361 cggtgttctg cgctttcgcc agatcctgcg cgtgcaccgg cgcggcggac aatgctgcgc
     6421 cggcggccca tatcaatgca cgtatcatcc cctcgcccct cgtcatcgaa aggcgcgatt
     6481 ttattagaat attctcgcaa gcacagtttc tcgccgcggc cggccgcccg ggcgatatcc
     6541 cgcccgcggg cccacccgaa gtcgcctttg ccgggcgctc gaacgtcggc aaatccagtg
     6601 cgatcaatgc tctcctcggg cgcaagcgcc ttgcttacac cagcaagacc ccgggccgca
     6661 cccaaacgat caatttctac gagttggggt ccgttgcgcg cctggtcgac ctgcccggct
     6721 acggcttcgc gcgcgcgccg cacgcgctgc gccggcaatg gaaagacctc gtcgacgcct
     6781 acctgcaatc gcggccgacg ctggccggca tcgtcgtgat catggacgcg cgccatccct
     6841 tcacggcgct cgacgagcaa ttgctggaat ggctcggaga ctcgcgcaag ctcgtgctcc
     6901 tgtcgaaggc ggacaagctc tcgcgtacgg aacaggccgc gctcatgaag agctacggcg
     6961 acgccttgct cttttcgagc actacgcggc aaggcgtcga ggaatgttgc aacctgctgg
     7021 aaagctggct ccagccggct gcagaaataa aaagcccccg gtaaagggga tataaaccgg
     7081 gggcaaaacg ccttaacttt gggttaaggc acccgctcag ggaggagaag cgggagacga
     7141 ttgactcgtc cgaaaggtaa gatggccccc gcccggcaaa gttcccccgt taacatttcg
     7201 aaataaatga acatttacgg caacttcccg gcgctgcgga tgcgccgcat gcggcgcgac
     7261 gagttctccc ggcgtctcat gcgcgagacc acactcacga cgggggatct gatttatccg
     7321 gtcttcgttc tcgacggcaa agcccgcact gaaaaagtgg cttccatgcc tggggtcgag
     7381 cgcatgacgg tggacaagct gctgccgctc gccgagcagt gcctgaagct gggcattccg
     7441 gtgctcgcgc tttttcccgt cgtggaatcc cgattgaaga ccgcggacgg gcgcgaggcg
     7501 ttcaatgcga aaggcctggt cccgcgcgcg gcggcggcgc tgaagaagcg ctttcccgag
     7561 ctcggcctga tgaccgacgt ggccctcgac ccctatacgt cgcacggaca ggatggcttg
     7621 atcgacgaga acggctacat cctcaacgac gccacgctcg acgtcctggt caagcaggcc
     7681 ctcgcgcagg ccgctgccgg cgtcgacatc gtcgcgcctt cggacatgat ggacgggcgt
     7741 atcggccgca tccgcgcggc gctcgagaaa gccggccaca tccacaccaa gatcatggcg
     7801 tactcggcga agtacgcgtc gggtttctac gggcctttcc gcgacgccgt cggctcggcg
     7861 aaagcgctcg gcaaagccga caagcgaacc taccagatgg atccggcgaa ctccgacgag
     7921 gcgctgtggg aagtcggcct cgatctcgcc gaaggcgccg acatggtgat ggtcaagccg
     7981 ggcctgccct acctggatgt tctgcgccgc gtgaaggacg agttcaaggc gccgacctac
     8041 gtctaccagg tgtccggcga atactcgatg ctgcgcgcgg cgatcgccaa cggctggttg
     8101 cccgagtcct gcgtgatgga aagcctgatc gccttcaagc gggcgggcgc cgacggcatc
     8161 ctgacttact ttgccttcga ggccgctaaa gtgctgcagt tgccagagaa agggtcttca
     8221 ggaagcgctc ggcgttctgg tacccgatca cgcgcagccc cttgatctcg cggccctcgg
     8281 cgtcgaagaa gacgatgccg ggcgggccga acagcgagaa gcgcttgagc agcgccttgt
     8341 cgttctcgtt gttggcggtg acgtcgacct ggagcagcag catccggtcg agctgtgagc
     8401 gaactttcgg gtccgagaag gtgaagctct ccatctcctt gcacgacacg caccagtccg
     8461 cgtagaagtc gagcatcacc ggcttgcccg gcgccttcag ccgtgtttgc agctcggtta
     8521 gggaggccac ccgggtccag gcgacaggtt gcgagtgcgg ggtgcctgcg gtgaacacgg
     8581 caagcgggcg caacggatcg cggctgcccg cgagagcgcc gacgagcagc gcggctccgg
     8641 cgatcaaggg cacggcggcg gcgatccggg agcggcgcag cagcgaggcc gcgccgaatg
     8701 ccagcaggct ccaagcgagc atgctggcaa gcggcggcag cacggggacg agaatccaca
     8761 ccgccaccgc gagcagcatc aagccgaaga aattcttgac cgtcgccatc caggcgcccg
     8821 cgcgcggcag cagcgcacct tcggaaacgc ccacggcgat cagcgggacc cccatgccga
     8881 gcgccatcgc gaaaagcgcg ctgccgccga ggaccatgtc gccggaacgc gagatctcga
     8941 gcagcgcgcc ggcgagcgga gccgacacgc atggactgac gatgaccgcc gacagcacgc
     9001 ccatcgcggc cactggaacc agccggccgc ccttcagttt cccgtgcgcg gtgtgcaggc
     9061 gctgctgcac cccatgcgga agccgcagct cgtacacgcc gaacatggag agcgcgagca
     9121 gcacgaaaat tgccgcaaag gcaccgagca cccacacgtt ctgcagatag gcggcgataa
     9181 gcgagccgac gtaggcggct gcgatgccgg caagcgcgta ggtaagcgcc atgctcagca
     9241 cgtaagtacc cgacagcagg agcgcgcgcg acttgtcgat gcgcgcgcct tctcccgcga
     9301 caatgccgcc gaggatgggg atcatcggca gcacgcacgg cgtaaaactg agcaggaggc
     9361 cgaaaccgaa gaagctggcg aggaccagcg ctgcattgcc ctggaagagc tgcgcgatat
     9421 ccatgtcgct cgcgaagatg gaccagcgac gctcggcatc gacctggcgc ggtcctgggc
     9481 tcatcaccgc ccccccggca tcccccgcga ccagccgcag cgaggcctgc gattccatcg
     9541 gcacatagca cacgcccaca tcggcgcagc cctgcgacac gaccttgagg tcgaagcggc
     9601 cctcgccttg cgccgggatg cggatgcgaa cttgccggcg gtaggtctcc acctcgccga
     9661 agaactcgtc cttgtggcgc gcgccggcgg gaaactgcgc cgcgcccagc cgcaccgccg
     9721 gattgccttc ggcggtgaag cggaacttgt cgcggtacat gtagtagccg tcggcgatcg
     9781 cgaagctgac ctcgacctcg cccgccgcgg cgcgggcgct gaagcggaac gccttctcgg
     9841 gctcgagcag ctcctcgctc ttttggaaca attgcgccga tcccggcgcg gcaaggatcc
     9901 acaacaacaa tccgatgagg aagcggttca cgccttggtt tccgcggcta cccagtcgag
     9961 ataggcgggc agtccgcgct cgattgggac cgcgatgatc tcgggaagtt cgtacgggtg
    10021 cccggcgcgc agcgcctgct cgagggccgc gtagcgctcc tgcgtggtct tgatgagcat
    10081 cgggtgctct tcgtcgtgct gcacggcgtc cttccagcga tacaccgagc ggcagggcgc
    10141 gaggatgttg acgcaggcgg cgaggcggcg ctcgacgagc atgtcggcga gcttctccgc
    10201 gacggcgcga tccggcacgt tggtgaagac gaggagcttg gtagactgca tgcggctatt
    10261 ttagcgatgc acaaagttac cgttcagccc agcggccagg ccttcgaggt ccaggaaggc
    10321 gaatcggtgc tcacagcggc gctgcgccag ggcgtcatgc tgccttacgg atgcaagaac
    10381 ggcgcttgcg ggtcatgcaa gggaaagatc gtcgccggcg ccgtcgacta cggtcactac
    10441 catgcgcgcg tgctgacgga ggccgagcgc gcgcacggca aggcgctctt ctgccaggcc
    10501 aagccgctcg gcgagctggt gatcgagtgc cgcaccatcg gcgcggccaa ggacatcgca
    10561 gtgcgcctgc tgccgtgccg cgtgcagaaa ctggagaagg tcgccgacga cgtcatgatc
    10621 gtgcggctca agctgcccgc gaacgagcgg ctgcagttcc tctccggcca gtacatcgac
    10681 ttcctgctga agggcggcga gcggcgcagc ttctcgatgg cgaacgcgcc gcacgccgac
    10741 gagctgatcg agctgcacat ccggcaggtc gccgggggca ccttcaccga ccacgtattc
    10801 ggcaagatga aagagcgcga catcctgcgc ctcgaggggc cgctcggcag tttcttcctg
    10861 cgcgaagaca gcgcgaagcc gatcgtgttc gtcgcaagcg gcaccggctt cgctcccatc
    10921 aagtcgatca tcgagagcgc gtttcaccgc aacgtcacgc gcccgatggt gctttactgg
    10981 ggcgcccgcc ggccgaaaga cctgtacctg agttcgatac ccgagacctg ggcacgcgag
    11041 cagccgaatt tccgctacgt gccggtgatt tccgaggccc ggcccgagga ccagtggagc
    11101 gggcgcagcg gcttcgtgca tcgtgccgtg atgcaggact tccccgacct ttccacgcac
    11161 caggtgtacg cctgcggcgt gcccgtcatg gtggactcgg cgaggaagga cttcaccacg
    11221 ctttgccagt tgcccgaaga cgagttctac gccgacagct ttaccaccca ggcggacctc
    11281 gcttcggacg aactgcgtta atgcttcttt ccctctaagc cgcgtcttct cccaggtagg
    11341 cttcctttac ccggctgtca ttcaagaggt ccgccgaacg acctgaaagt gagatcacgc
    11401 ccgactccat gacgtagcct cgatgcgcga gctcgagcgc gaggcgcgcg ttctgctcga
    11461 ccagcaggat ggtgacgccg cggctggcga tgtcgcgcac cacctcgaag atgcgcgcca
    11521 ccatcatcgg cgcgagcccc atcgacggct cgtcgagcag caagagcttc gggtcgctca
    11581 tcagcgcgcg cgcgatggcg agcatctgct gctcgccgcc ggagagcgtg cccgccacct
    11641 gcaggcggcg ctccttcagc cgcggaaaag tcgcgtactg cttctccgtg tcggtgctgc
    11701 cgcgcgcata ggcacccatg gccaggttct cctcgaccgt gagctgcgga aaaatgccgc
    11761 ggccttcggg caccatcacc agccccctgc gcggcagctc gtgcaccttg gccgcggaaa
    11821 tatcgcttcc ctcgtaagtg agctttttcg atcgggacgg aatcaggcgg cagatcgcct
    11881 tgagggtggt ggtcttgccg gcgccgttgg cgccgatcag gcagaccagc tcgccttcct
    11941 tcacttcgag atcgatgccc ttgacggcgc gaatgccgcc gtaatgcacc tccaggtctt
    12001 tgacttcaag cagcatgcgg ggcaccgagg taggcctcga tgaccttggg gtccttctgc
    12061 acctcggcgg gcgttccctc ggcgatcctc tcgccgtagt cgagcaccag gacgcggtcg
    12121 caaacgttca tcaccaggcg cacgtcgtgc tcgatgagga gcacggtgac gccgtcgtcg
    12181 cggatgcgcc ggatcagttt gccgagcgcg atccgctcgg cgggattcat ccccgcagcc
    12241 ggctcgtcga gcgccagcag cttcggctcc atggcgagcg cgcgcgcgat ctcgagccgg
    12301 cgctggtcgc cgtagggcag gctgccggcc gcgtcgttgg cgcgcgcgcg gatcccgaca
    12361 tagtcgagga gctccagcga gcgtttttcg atttctcttt cctctttcag cgtgcgcttg
    12421 ttacgcaaca ccgcgcccac cacccctgcg cgcgtgcgcg cgtggcgccc gatcatcacg
    12481 ttctcgagcg ccgacaggtt gacgaaaagg cggatgttct ggaacgtgcg gccgatgccg
    12541 gcggcggcga cctggtgcgc cttgagcccg gcgagcgcgc tcccgtcgaa ggtgaacgcg
    12601 ccggcgtcga tcgggtagat cccggtgagc acgttgaaca acgtcgtctt cccggcgccg
    12661 ttcgggccga tcaggccgta gatctggccg cgtgcgatcg agaacgaaac cgcgttcagc
    12721 gcctgcacgc caccgaagcg cttgctcacc ccgtccgcgg tgaggaggtt cacgccgcgc
    12781 gttcctcgag ctcgcgcttg cgcaccgccg atggcagcag gcccgcgggg cggtagagca
    12841 tcaccagcac cagcgccagg ccgaagagca gcatgcggat gacctccggc tcgatcagca
    12901 tgcggccgaa gagcgcctcc tgcaccgggg tgaccgtcca gcgcaggatc tcagggacga
    12961 acgacagcag cagcgcgccc aggatcacgc cccagatgtt ccccatgccg ccgagcacca
    13021 ccatcgacac caccatgatc gattcgacca gcacgaagct ttccggagag atgaaggcct
    13081 ggatcgccga gaacatgccg cccgcgatgc cgccgaagct cgcccccatg gcgaaggcga
    13141 gcagcttgag attgcgcgtg tcaattccca tggcgcgcgc cgcggtctcg tcctcgcgga
    13201 ttgcctccca ggcgcgcccg atgcgcgagt tctgcagccg caggttgacg aggatgatgc
    13261 cgagcgcgac cgcgagcagc aggtagtagt acttgatcgg gctatcgaag cgaaagccgc
    13321 ccaggacgaa gggatcgatg ccggtgatgc cctgcgggcc gttggtgatg ttcaccggcc
    13381 gcgacagatt gttcaagaaa atccggatga tctcgccgaa gccgagcgtc acgatcgcca
    13441 ggtagtcgcc gcgcagcttc agcgtcggcg cgccgagcat cacgccgaag agcgcggcga
    13501 ccgccgcgcc gagcggcagg atcacccaga acggcaggtg caggccgagc tgcgggcttg
    13561 cgagtaatgc gtagacgtac gcgcccaccg cgtagaaggc gatatagccg aggtcgagca
    13621 gtccggcgaa gccgaccacg atgttgaggc cgagggcgag gaggatgtag aggatggcgt
    13681 aattggtgat gcgcacccag gtggtgccgg cctgcgcgat ggcgaacggc agcacggcaa
    13741 gggagagcgc aatgagaaag attccaaccc aggcgagcca cggcttgccc ttggtgtcga
    13801 agtactgccg catcaggcgc ggtctcccac tctttcaccg agcagcccct gcgggcggaa
    13861 cacgagcacc aggatcagca cgatgaaggc gaacacgtcc tggtagttcg agcccatcag
    13921 gatgaagtgc ccgccgccct cgcaggcctg cagcggcagc ccccattggc acaggtcggt
    13981 gaagtcgccg atgtaaccgg cgccgagcgc ctcgaccagg ccgagcagca cgccgccgag
    14041 catcgcgccc gggatgttgc cgatgccgcc gagcactgcc gccgagaacg ccttcaggcc
    14101 gagcaccgag cccatggtgt actgcgcgat gccgtagtag gtgccgaaca gcacgcccgc
    14161 taccgcgccc agccccgcgc cgatgatgaa ggtgatcgcg ataacccggt tgatgtcgat
    14221 gcccatcagc ccggccacct gcgcgttctg cgcggtcgcg cgcatggcgg tgccgagcgg
    14281 cgtgcggtag acgaggatcg cgaggctcgc catgagcgtc acgcagatcg cgaggatcgc
    14341 gatctgcacc gtagtgatcc cggcgccgcc gatgaagtag gtctgcgact tgatgatctg
    14401 cggaaacgcc agcacgttgc ggctccacac cagcatcgcg aggtgctgca ggatgatcga
    14461 cacgccgatc gcggtgatga gcggtgccag gcgcggcgcg tgccggagcg gccggtaggc
    14521 gaggcgctcc atcgaatagc ccaccagcat gcagatcggg actgcgcagc cggtgccgat
    14581 cagcacgatg gcgagcgccg gcaggccggt gggtgcgagg aggttgatga tggtaaaggc
    14641 gaccatcgcg ccgatcatca ccacttcgcc gtgcgcgaag ttgatgagct ggatgatgcc
    14701 gtagaccatc gtgtagccga gggcgacgac ggcgtacacg ctgcctgcgg tcaagccgtt
    14761 gaccagctgc tgcaggaacg tgtccatgcc gggccaaagc ctagcagaaa cgaaaacggc
    14821 acccgaaggt gccgttgtcg tgagcggcta cggcagggct atttcatgcc cttcacgaag
    14881 gcgtcgaagc tctgcgactt gccgccggtg atcaccgcca ccggcagcag cttgccgccc
    14941 ttcatggtga agatggtcat ctcggcgtcc ttgcggtcgc ccttgctgtc gaacgcgatc
    15001 ttgccggtga cgcccttgaa gtcgagcttc tgtagctccg gcaggtactt cttcgggtcg
    15061 ctcgagttcg ccttctgcat cgcctggatc agcatgttgg cggcgtcgta ggtgaacggc
    15121 gagtagagga tcgggtcggc gttgaacttc ttcttgtaag cctcgaggaa cttcttgtcc
    15181 gccgcctcga ccgggatgcc ggcctgcgag cagagcagcc cctcggcggc gtcgccggcg
    15241 agctccttca tcttgtcggt gcaggcgccg tcgccgaacg agaacaccgc cttgatgccg
    15301 agctcgcgtc cctgcttgag cagcgggccg ccggtcgcgt ccatgccgcc atagaacacc
    15361 gcgtcggggt tgcgaccgcg cagcttggtc agcaccgctt tccagtcggt ggtcttgtcg
    15421 gtgcccttct cgcgcggcag cacgttgatc ttcgccgcct tgagcgtctt ctccacttcg
    15481 ttggcgatgc cctcgccgta ggcggtggcg tcgtcgatca ccgccacggt ttgcggcttg
    15541 ttgttggtgg cgaggtagct cgcgatcgcc gggccctgct ggtcgtcgcg gccgaccacg
    15601 cggaattggg tcttgaagcc ctgctcggtg agcttggggt tggtggccga gccggagatc
    15661 accgggatgc cggcgccgtt gtagatcggc gaggccggga tcgaggtgcc ggagttgagg
    15721 tgcccgacca cgccggccac cttggcgtcg accatcttct gcgcgaccgt cgtaccgact
    15781 ttcgggtcgg cctggtcgtc ttcggcgacg aaggtgaact tcaccgcctt gccgtcgatc
    15841 ttgatgccgg ccgcgttggc ctcttcgacc gccaggcggg cgccgttctc gttgtccttg
    15901 ccgaggtgcg cgatgccgcc ggtgagcggc ccgacgtgtc cgatcttgac ctcaaggccg
    15961 ccggcggcgg caggggcgac gcctttgtcg gccgtggcgg ccggcttctg gtctccgcag
    16021 ccgatcaggg cgacggcggc ggccaatagg gtaatgctgg aaatcttgtt catggggtct
    16081 cccgggggta gtg
//
LOCUS       DATBSZ010000019        35285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_753418, whole genome shotgun sequence.
ACCESSION   DATBSZ010000019 DATBSZ010000000
VERSION     DATBSZ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 35285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35285
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753418"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VIG70_01145"
     CDS             complement(<1..426)
                     /locus_tag="VIG70_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249181.1"
                     /translation="MKAILSALLVCAAVAAHADEVSLRLGGKEIDLTPKLRQELSELA
                     RQALARCGPNTSRHPGNFGLAAFGVESRWKALLEGSRLRVRFSEPFLSESHLGGTLGV
                     SEALIGLEHKEFFVGPDFTRHGAAVAEHLQCGYLPLLELA"
     gene            complement(423..1130)
                     /locus_tag="VIG70_01150"
     CDS             complement(423..1130)
                     /locus_tag="VIG70_01150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8249182.1"
                     /translation="MGRLQGKAAFVTGAGAGIGRAIADALAREGATVARVDIDGSTPY
                     QCDVSDRAAVFATMKRFVAEAGGLDILVNNAVSFHYAPLVDMPEDKIHRMLDVGIKGV
                     FWCTQAATPHLIARGGGAIINLSSVAVSFGITNACVYSSIKGAIDTFTRQQAVELAPH
                     GIRVNALAPGPVSTPGSNSVIDAAGWEKRRSRTPLQRLAGADDVAAAAVFLASEESRC
                     IAGVTLKIDGAITVVGP"
     gene            1168..2262
                     /locus_tag="VIG70_01155"
     CDS             1168..2262
                     /locus_tag="VIG70_01155"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8249183.1"
                     /translation="MKRIVFAGLLAAALSAPVLAQTKVQMQSAFPPRGTFADNLVFFA
                     ERVKVMSAGRLEIVPLPTGQIVPAFEVLDATHKKVIDGAHSSSAYWVGKNRAAALFGV
                     TPGGPFGMDGFDYMGWLYDGGGMELYRELFQVVLQRNVFPIPMTSAGPQALGWFKRPV
                     KSWADFKGLKCRQTGISAEVFVKSGMTTVNMAGGEIVPSAQRGVIDCAEWVGPSDDML
                     MGFHQVWKHFYTQSSHDLSLLEIIINGDVWKALTPDLQAIVQAGAIEATLRSQNRRNR
                     LDADALEELRTKHNVVVHRTPPDILKNMLLAWDGIAAEEAAKNPFFKKVYDSQRAYAS
                     KVVTSRLVTAPSYNAIAGHYWPQQPAQKKK"
     gene            complement(2263..3726)
                     /locus_tag="VIG70_01160"
     CDS             complement(2263..3726)
                     /locus_tag="VIG70_01160"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY8249184.1"
                     /translation="MGHIESQKLWNEYERPHADLREAIERLERAGELRRIRGADWKLE
                     MGTLAEIVYREESPPAILFEDIPGYPKGFRALSGATNSAKRLALLLGFPVPSHPLDVV
                     RAYRDRMKTHEPIPPKTVARGPVLENILKGKDVDVLRFPAPLLHEHDGGRYIGTDDLV
                     IMRDPEQHWVNCGTYRAMVQGPDRLGLWISPGKHGRQIREKYWKQGKPCPVLISCGHD
                     PLLFLAGGNEIRFGLSEYDYAAGHRGMPYEVIESELYGLPMPAHAEIVLEGAMHPGET
                     APEGPFGEFTGYYAGGRTPQPVVRVERIYHRNDPILGLACPMVPPSDFSYSKCVMKAG
                     MIWDEVERAGLAGVRGVWVHEPGCARMFNVISIKQAYAGHARQAAVLAASCQSGAYLG
                     RFVVVVDDDIDPANLDQVIWAMCTRCDPAEDIDFIRRAWSGPLDPRLPKGVTWNSRAL
                     IDACRPFEMLGDFPPVVKASPELREKVLRKFEDQLKP"
     gene            complement(3713..4609)
                     /locus_tag="VIG70_01165"
     CDS             complement(3713..4609)
                     /locus_tag="VIG70_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HEY8249185.1"
                     /translation="MNAPEKLKQYNRDAQVVGNIVHLEHFNVIVGDQRFATLFYVVGL
                     GGTRDPYIFSGLENMWVNFGRTQVHLPNRGGKAPEVLRGTAGFVVPSLEEAKKRLEHA
                     GNEMKRIAPDVKTRFSWREKEGTIEATCPWGNRVRLHAPSPEFGRTELALAYVDFDVP
                     QGTAEGIARFYNEIMRAPARFDKNRTSVSIGRDQRLFFTETAAPQPEYDNHHIQIYIA
                     DFASPYHWLKERGLINMETDAEEWRFQWITDPRDGRKLFQIEHEVRSMKHRLFARPLV
                     NRNHGVTNLNFVPGADAFRGTY"
     gene            complement(4633..5304)
                     /locus_tag="VIG70_01170"
     CDS             complement(4633..5304)
                     /locus_tag="VIG70_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA synthetase"
                     /protein_id="HEY8249186.1"
                     /translation="MHSREELLACVIARLIGGARHVAVGAASAIPATACELLKARGAP
                     LRVSMLHKRRGNPFTEGSRELFDLAGQGRIDVFFLGGAQIDGEANINLVRAEGRRFPG
                     SFGSAFMYSVVKNTILFREEHSPRVLVPKVEFISARGDPAALVTGKALFSWQKDKRRF
                     RLESVHQPGDVRADTGFSYDAPDKLGLTPAPSAEDLTLLRGPVARAIAADYPDFAKRV
                     WGLTT"
     gene            complement(5259..6095)
                     /locus_tag="VIG70_01175"
     CDS             complement(5259..6095)
                     /locus_tag="VIG70_01175"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY8249187.1"
                     /translation="MAAQPEDLARLIPDGALLALPPDYSMPAMAIVRALIVQKKRNLR
                     LLGVPVLGLCADLLIGAGCVAEVESSAVSLGEAGLAPRFSEAVEKNLIKVKDATCPAV
                     HTALQASEKGVPFMPLRGVLGSDLVKHRADWQVGRNPFNPEEDPILLVPAIQPDIALF
                     HARWADEAGNVWVGRRRELATIAHAAKQTFVTFEELKPGDMLEDELLAPGVISATYVS
                     AIAPAKRGAWPLGIPGVYDIDDAHLAHYARAARTQEGFQRYLDEYVCIPEKSSSPASS
                     PA"
     gene            complement(6079..7509)
                     /gene="tldD"
                     /locus_tag="VIG70_01180"
     CDS             complement(6079..7509)
                     /gene="tldD"
                     /locus_tag="VIG70_01180"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEY8249188.1"
                     /translation="MILETAKTTLLAPFDLETQKLERVFGTLAMRKVDYADLYFQYSR
                     FEGWSLEEGIVKSGSFNIEQGVGVRAVSGEKTAFAYSDEISFSALQDAAKATRAIASA
                     GQARRVKAAARKVPVPLYRDIDPIASLASESKVALLEKLEKKCRALDPRITQVMASLG
                     GEYEVVLIARADGAIAADVRPLVRLSVQVIAEQNGRRESGSSGGGARTDYSHFTDDVL
                     DAYARQAVSQALVNLEARPAPAGTMTVVLGPGWPGVLLHEAVGHGLEGDFNRKGSSAF
                     SGRLGERVASPGVTVVDDGTMPGRRGSLSVDDEGTPTQRNVLIEDGVLRGYLQDSLNA
                     RLMKMPLTGNARRESFAHVTMPRMTNTYMLAGPHVPAEIIKSVAKGIYATNFGGGQVD
                     ITSGKFVFSTAEAYLIENGKVTHPVKGATLIGNGPDALTRVTMIGNDLQLDPGIGTCG
                     KEGQSVPVGVGQPTLKIEGLTVGGTA"
     gene            complement(7506..8291)
                     /locus_tag="VIG70_01185"
     CDS             complement(7506..8291)
                     /locus_tag="VIG70_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY8249189.1"
                     /translation="MARARIAAIQMVSGPEVAANLAAAERLIAAAAAAGAKLAALPEN
                     FYLIGRHEGDKVALREPDGNGPIQQFLSEVSKKNRIWIVGGTAPISTDDERRIRGACL
                     VYDDTGKRAARYDKMHLFRFEGGGKESYDESRTLQPGTRPLAIASPFGRLALSVCYDV
                     RFPELYRGLGEFDVMFVPSAFTVPTGAAHWETLLRARAIENKAYVVAPAQGGTHASGR
                     RTWGHSMIVDPWGEILASRDEGEGVVLAEIDHEKLKEIRRRLN"
     gene            complement(8291..12073)
                     /locus_tag="VIG70_01190"
     CDS             complement(8291..12073)
                     /locus_tag="VIG70_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HEY8249190.1"
                     /translation="MKRLLRAVEVLAWGGFFAFAALVLAVRFWVLPDIERYREHIVAA
                     MSRGIGLPVKVGEIRAGWLGLRPHIGLSDVRIYDAQGREVLVLPSIENVVAWRSLAHG
                     ELRLHSMAIEGLRLGVRRDAAGDFFVAGLKLEKPREPSGGGFGAWLAGQGQIVVRNAE
                     LEWRDELRGAPPLVLSGLNVQVEASVFSLALGVTARPPAELGSSVEVRALVDARGLQR
                     GEWSARVFGQVGNTDLAAWRAWVDYPFNVRQGQGALRFWSSVERGELKEFTADLALAD
                     VRASLGDVLDPLELVSVHGRISGRWLADGIEFSGRGLALAMEGGPEVPKTDFQIAWRP
                     QAGGTLGASLVDLEAIRDLAGSLPVPPQISQAVKQFAPQGRLTDFRLEWSGPFDAPTR
                     VGVRSRFAELALRAREELPGFSGISGSLEADLDKGKLSLASHKSVLELPRVFPDPHLA
                     LDSLAGQVEWERDASGGLTVRIGSLTFANAHASGNVYGSYARRGDGPGSIDLSGVLNR
                     ADGRQVGRYLPSILQGDVRTWLANSILAGEASDVRVRIRGDLREFPFVDPSTGQFQVT
                     ARVEKGVLEYAAGWPRIEDIAGELNFERDRMEIVGRSGSILGAQLSNVRVAMPSLRSP
                     ERHVQVSGQADGPTAEFLRFLQSSPLRETAGRFTEDIKAAGRGKLRLKLDLPLYALPK
                     TRVAGDYEFAANEVNVLPWLPPIEAASGRLAFGESGFTLHDVRGRVFGGEVGISGGTR
                     AGRGIEVLARGNAALEAARTLAGSMRDHPLGKHLSGTFDYFLTVRAQDGVARVTFESP
                     LRGVESTLPAPLAKSAAEPLPLRVEINPAPKGERDRISVTLGALARAEVQRRSQDGTM
                     VVQRTAVWVNPERDQPIRLPERPGTLVYGSLAAFDLDRWLPLAGGDGGGSSETISVEM
                     KFGTLDAFGRRLSNVGLRASTAAAGWSANVKADELAGDVSYRTRPQPRVIARLAHFTI
                     PADTPGAQPRPTGRPSELPDLDLVADEFTFRGKPLGRVELIARRAGKDWRIESASMIN
                     ADASLTGSGIWRAEPSRSEVQFDLEAGDAGSFLTRVGQPGMVKGGRTRMRGALAWQGD
                     PATLDFPTMSGEVQMQAENGQFLEIEPGLGKLIGLMSLQALPRRVTLDFRDVFSKGFQ
                     FDRIASNAQVDSGVVRLREFRMRGSAADVEMSGEADLAHETQDLRVRVLPSLSDSAAL
                     GVALVNPVAGVAAAIAQRILKNPLGQIFAFDYTVTGSWSDPKVVKILPPPPTELAN"
     gene            12111..14633
                     /gene="glnE"
                     /locus_tag="VIG70_01195"
     CDS             12111..14633
                     /gene="glnE"
                     /locus_tag="VIG70_01195"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEY8249191.1"
                     /translation="MPELTSQTASKIRLSRFAQRVLSARPELAAELADPQPFTRAEML
                     NALGGSGDDGEAQLKRSLRLLRNRVLLRVMARDLSGRAGLDEVCGTMTDLAEVAVSRI
                     LNQENLIVVGMGKLGGRELNVSSDVDLVFLYRGPAEAQERYERAGRRLIRLLSEPTED
                     GFVFRVDMRLRPYGDSGPLACNFEALEHYLITQGREWERYAWLKARPLGASDELRELD
                     AIVRPFVFRKYLDYGTFAALRALHAEVRRDVARRELAEHVKLGPGGIREIEFIVQAQQ
                     LVRGGRDPELRVRSTVRALAVLSEKSLLPESAAREMADAYVFLRNLEHRLQYLDDAQR
                     HDLPADGEDRARIAEMCGFATWEGFYQALQRVRSTVSRHFDAVFAQPDVPAEPWPEHP
                     RLAALRASQRYAALPEESRRRLDRLMPALARAAQATPDPGATLARGADLVEAIASRAA
                     YLALLAEHPEALERVARMIGSSSWAAEFITRHPLLLDELLDDRLLYAPPELVSFQKTL
                     RSQLAAARDDTERRMNVLREMHQAQVFRLLAQDLAGLLTVEKLADHLSALADLMLEVT
                     LELVWEDLRGKHLPAPRFAVAGYGKLGGKELGYASDLDIIFLYDDPDERAPEVYARLA
                     QRLNTWLSSRTSSGMLFETDLQLRPSGASGLLVSSIQAFERYQEENAWVWEHQALTRA
                     RYCAGDRQVGAAFEQIREKILRKPRNAAELRASIVEMRAKLHAAHPNKSGLFDVKHDA
                     GGMIDIEFAVQYLVLQFSREFASLTANLGNIALLKMAADHGLIDAGLAERCRDAYREF
                     RRIQHALRLNGAQYARVPHERVSAERESVQALWQAVLATSDP"
     gene            complement(14617..15036)
                     /locus_tag="VIG70_01200"
     CDS             complement(14617..15036)
                     /locus_tag="VIG70_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEY8249192.1"
                     /translation="MNQFRFSRVARRIAALCGRPATFVLAVTVIVLWGLGGPLFGYSN
                     TWQLVINTTTTIITFLMVFIIQNTQTRDTEAMQLKLDELIRATQGAHNALLDLEDLEE
                     AQLERYRRRYEALAAMAREKLERGELDTDSPEIKDLK"
     gene            complement(15033..15806)
                     /locus_tag="VIG70_01205"
     CDS             complement(15033..15806)
                     /locus_tag="VIG70_01205"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEY8249193.1"
                     /translation="MKRSWTAAAMLLGACASTPDDPLVWSDDLVRSLPRERGEVVEVA
                     RFSRVPPGASPAPWEQWLIRRGNHPTDYRVVEVDGTNVLEADAQEGGSGFSRKIRVDP
                     SRHPVLEWRWRANAEPGTRLEMTSGTSPLARLSLAFHGDANKLDFDDRAKLRLAMVLT
                     ANGLPYASLVYVWMLDVPAETVIRSPYTDRVRYLVVESGAQRVGEWVSVRRNVVEDYR
                     RAFGEEPDDIVAVGVMTDPGDDFSRRRSFYGDITFRSGK"
     gene            complement(15819..16118)
                     /locus_tag="VIG70_01210"
     CDS             complement(15819..16118)
                     /locus_tag="VIG70_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8249194.1"
                     /translation="MLVNNPQRVFYALASLPRRRILAFLSQTALSTADLAERFGISRP
                     AISRHLSVLQRAGLIAGERQGQRVLYRLKRDALLEALARFASEVDGPLRTPRNAV"
     gene            16231..17160
                     /locus_tag="VIG70_01215"
     CDS             16231..17160
                     /locus_tag="VIG70_01215"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY8249195.1"
                     /translation="MSMADRDGWIWHDGKLVEWRSATTHVLTHSLHYGLAVFEGVRAY
                     KTAIGTAVFRLKEHTDRLFSSAHIYMMKIPYTRERLMQAQLDVVQANGHDACYIRPIA
                     FYGSEKMGVSPVGAKVHVAIAAWPWGAYLGPEALSKGIRVKTSSYARHHINVTMARAK
                     MSGTYPNSVLATLEATMHGYDEGLLLDVDGFVAEGAGENIFVVKNNKLYEPELTSALT
                     GITRTSVIELAAELGYPIISKRLTRDDIYIADECFFTGTAAEVTPIRELDARVIGEGK
                     AGPVTKAIQKAFFDVVTGKDKKRRSWLTPVKSK"
     gene            17133..17318
                     /locus_tag="VIG70_01220"
     CDS             17133..17318
                     /locus_tag="VIG70_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEY8249196.1"
                     /translation="MADPRQIEVTEKDLPLHCPTPGTALWALHPRVFLDVAKEGETLC
                     PYCGTKYVYKGAPVKGH"
     gene            17319..18239
                     /gene="waaF"
                     /locus_tag="VIG70_01225"
     CDS             17319..18239
                     /gene="waaF"
                     /locus_tag="VIG70_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEY8249197.1"
                     /translation="MRILVVAPNWIGDALMAQPLLARLKQSGATIDVMAPEWVAPVVR
                     RMPEVEDVIPAAFRHGPLQLGERWRLGRSLKRRGYHRAFVLPNSWKSALVPFFAGIPE
                     RVGYVGELRYGLLNSLHKSTQAPMPLHYARLAGEPVGVPKLQVTEQEILDARRRFGIE
                     GAYVVLCPGAEYGPAKRWPYFKELAEKLPAAVVLGSSSDRSAAAGMAGKNLVGETTLD
                     EAIRLIAGAAAVVSNDSGLMHVAAALGRPQVALFGSSSPEHTPPANDKARVLWLRLEC
                     SPCFERECPLGHFRCMKEISVEQVLASLNP"
     gene            18236..18676
                     /locus_tag="VIG70_01230"
     CDS             18236..18676
                     /locus_tag="VIG70_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8249198.1"
                     /translation="MTGKIFPTAQDAENAFYESLERCDLEGMMAVWSEDEEIVCVHPA
                     GARLTGQDEVRESWARMFAAGPRARLAIERQVAISGMMLAVHSVFESFTIPQAKPEAQ
                     PAPIIATNVYLRTAAGWRMIVHHASPAPAQPQAAPRDAPPKILH"
     gene            18677..19606
                     /locus_tag="VIG70_01235"
     CDS             18677..19606
                     /locus_tag="VIG70_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8249199.1"
                     /translation="MFRSPWWLPGGHLQTIYASLRRPPRVELERRRWETPDGDFIDVD
                     FAGDAAAPRRLALFHGLEGCSDSHYARAIAAHAARRGWRIALPHFRGCSGEPNRKPRA
                     YHSGDTQEVDWILRKIDSPFVIGISLGGNALLKWLGERGDDARALVQRAAAVSAPIDL
                     PAAGRALDRGLNRLFYTRHFLSTLRPKSLAKLERFPGLYDAARVRAARRFREFDDTVT
                     APLHGFRDVDHYWSEAASGPWLERIRVPTLLLNARNDPFLPEHALLAAARKAAPCVVL
                     EFPRTGGHAGFVSGPFPGQHGWLARRLLDFLDQ"
     gene            19603..20976
                     /locus_tag="VIG70_01240"
     CDS             19603..20976
                     /locus_tag="VIG70_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY8249200.1"
                     /translation="MNVPAEIFRAYDIRGIVGRTLTAPLVREIGRALGTVARERGAPA
                     FAVCRDGRLSGPELANALMEGLTAAGADAIDIGMGPTPLAYFAAHHLGCGSCVAVSGS
                     HNPPDYNGLKIVVGGDALYGDDIQDLRTRTESGKFASGKGKRSTADVLDAYVERIAGD
                     VKLARKFHVAIDCGNGVAGMLAPRLYRRLGCEVTELFCEVDGTFPNHHPDPAQPKNLV
                     ELIRTLKTGKAELGLAFDGDGDRLGVVTKDGEIIYADRQLMLLAKDVLSRNPGAEIIY
                     DVKSTRLLAPWIERHGGKPVLWKTGHSLIKAKLKESGALLAGEMSGHTFFKERWYGFD
                     DALYGGARLLEVLSREKDANPVLKNLPNAPSTPELHWELEEGEPHALVARLQESQPFP
                     GAERVVTIDGVRVEYADGFGLARASNTTPTIVMRFEADNSAALERIKKDFRAALQPLK
                     PGAPLPY"
     gene            20957..21481
                     /locus_tag="VIG70_01245"
     CDS             20957..21481
                     /locus_tag="VIG70_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY8249201.1"
                     /translation="MRHFLISLLLGGNTAMAACPALLDRKMDTILDQPQSLCEYTGKV
                     VLVVNTASQCGYTPQYEGLEALYRKYRDRGLVVLGFPSNDFGGQEPGSNKDIAAFCVN
                     QYAIDFPMFAKTELKKNPLYADLAKATGAAPRWNFHKYLIDRGGKQVQSFDTRVDPND
                     PKLTAAIERLLAAQ"
     gene            complement(21497..22774)
                     /locus_tag="VIG70_01250"
     CDS             complement(21497..22774)
                     /locus_tag="VIG70_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8249202.1"
                     /translation="MLRIGARIFSLLVSLLCGASALAADIRLWHSMNAARGAEFERIV
                     ARFNASQKQFRVAATYKGAYDEAAVEAVTARGSRNFGARAPHIVQASELGGAFLLDQK
                     DAARPLWQVMPEAGAVLRAEAALGGAEELVDAEGRLLALPLGSATPVLYYNRDAFRRA
                     GLDPAKPPATWYDMVPTLAALLASGSDCALTMAWPASTLLENMAAWHNQAFATDRLMF
                     NNRLAVRWVSTLATWQKSGYFTYAGGRGEAEARFVAGECALLAASSANYDELRGRAKF
                     DVGIAQFPYYDDFDGAPLNTLASGSAFWVMAGRPAADYAGVARFLAFFARPEVQAEWH
                     QRTGLVPLNAAAYEITRKAGFYKAHPGHEIAVRQLLLRGTPNWKSLRLGQFPKLRSII
                     DEELESTWQGGKSPIDALNHAVARGNAFLDGTR"
     gene            22802..24193
                     /locus_tag="VIG70_01255"
     CDS             22802..24193
                     /locus_tag="VIG70_01255"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8249203.1"
                     /translation="MPALHERSAAELAAAFGCGELSPLEAARAALDRIEAWEPRINAM
                     YRVHREAALEQARASEARWRSRRALSALDGVPVTIKENIYTRGDPAPVGTRANEDAPP
                     QAADAPAAARLREAGCVILGKTTMPDFGMLSSGLSSLHGVTRNPWRLDRNPSGSSSGA
                     AAAAVAGYAPLHVGTDIGGSVRLPATHCGLFALKPSLGRVPVNPPYMGRVAGPMTRSV
                     HDAALLMSVIAQPDARDFMSLPDQELDFPALQPLAAKGLRIGFVPDMGAGLDVHPEVR
                     AAAEAAAKALAGAGCEVGSIRSFLTREMLDGMCRFFEARSYNDLMQLPPAKREKVLPF
                     VAEWCSWRGAHFSGRDVMQAYALVMSMREAAVAASEPYDFILSPTSPILPYEAELPAP
                     GNDPCDALPHIAFTVPYNMSEQPAASINWTYSSEGLPIGVQVVGRRFDDAGVLRMSAL
                     LEGLRPQQRPWPQ"
     gene            complement(24161..24529)
                     /locus_tag="VIG70_01260"
     CDS             complement(24161..24529)
                     /locus_tag="VIG70_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEY8249204.1"
                     /translation="MKLAAEISVLLVALLHIYFLVLEMFLWDKPFGRRTFGMTPEHAA
                     ATKTLAMNQGLYNGFLAAGLLWGLAAGAQVTVFFLVCVIVAGVFGAATASRKILWVQA
                     VPGLLALVLVLLRPGPLLGT"
     gene            complement(24529..24789)
                     /locus_tag="VIG70_01265"
     CDS             complement(24529..24789)
                     /locus_tag="VIG70_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249205.1"
                     /translation="MNSEKIKVRNTDTGQVIEVVVFSKRAESIEVVLGEGVHNVKCTL
                     TPTRNGMAYAGKIMGREIVYERSRDQVQGDIDRLDPRLKKSR"
     gene            complement(24786..26273)
                     /locus_tag="VIG70_01270"
     CDS             complement(24786..26273)
                     /locus_tag="VIG70_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEY8249206.1"
                     /translation="MARIAIGGFQHETNTFAPSKADYAAFEAGGGWPGVRYGEPIFAA
                     VEGANIPAAGAIHALRSLGHSLVGTAWGAASPSAHVTADAFERISTELVKRLRAELPL
                     DGVYLDLHGAMVVETYDDGEGELLRRVREAVGPRVPVVASLDLHANVTRAMFERADAL
                     VAYRTYPHVDMAETGARAARVLDQMLRAGQRPQGGFHQLDYLTGIPSQCSFIEPCKTI
                     YQELSKLEERHGCVLSFTPGFPMADFADCGMSVFGYGSVGALEQLRGAVADAEKDFAM
                     ELHEAPLAVRRARSVGEPGAPVVLADTQDNPGAGGNGDTTGLLRSLIDQNASDAVLGM
                     LIDPASAQRAHEAGQGSSPTFSLGGRSRIPGDAPLSGEFTVERIGDGRFTCSGPMFKG
                     FRMTLGPMALLRSRAAPGVRVVLASRKCQAADQEMFRTLGVEPRKSRIVALKSSVHFR
                     ADFEPIAKEVLVVKSPGPALADPAEFKWSKLRKGVRLRPLGPVHA"
     gene            26314..27900
                     /locus_tag="VIG70_01275"
     CDS             26314..27900
                     /locus_tag="VIG70_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8249207.1"
                     /translation="MRYRVLAAALVLSASLGVAPGFSPLHAQTKVLRVVPHSNLNILD
                     PIWTTQYMARNHGYMVYDTLFGTDEKSRIQPQMVERWTVSKDQKEWSFTLRGGLEFHD
                     GKPVTGEDVIASLQRWGKRDAMGQKLMTFVERMDSPSPASFRIVLREPCGFVLEALGK
                     PSSNVPFIMPKRVADTPADKQIEDAIGSGPYIFKKDEFKPGDKAVYTRNDKYVPRKET
                     PSGTAGGKHVYVDRVEWNLALRDAQSQVNALANGEVDVLERPAFESYEQLKTAPNVQL
                     VTKDPLGFQYMCRFNHLHPPFNNAKVRQAALAAMAQEPFLRAQVGIKEFYRPCASMFT
                     CNTPYGSSKGSEIQSKSSMKKAQELVKSSGYDGTAVVIIKPTDLAAIQKLPDVAAQLL
                     RQAGFKVDLQAMDWNTVVSRRAKKEPPAQGGWNIFCTAWVAPDIWNPLTNAAVGAQGE
                     KSWFGWPSDAQLEKLRDEFARATDEGKKKGLAEAIQARAFEIGTHAPLGEYVNPLATR
                     KNISGWVIGPGDIYWNVKKN"
     gene            27906..28847
                     /locus_tag="VIG70_01280"
     CDS             27906..28847
                     /locus_tag="VIG70_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8249208.1"
                     /translation="MVNFIARRLAATVPVLLVVAIFVFLLLRLTPGDPAAIMAGDAAT
                     AEQIESIRAGLGLDRPIVVQFGLWMQKILSGDLGESFYFRIKVATLIGQRLEPTFALA
                     TLTILLAVAVSIPLGVVAAWRFGGWFDRALMGFSVLGFSIPVFVLAYLLIWLVSLKLG
                     WFPVQGYRRLADGFFPFLHHLVLPAVTLSVIYIALIARVTRASVLEALGEDYIRTARA
                     KGLPELRVLVRHALANAAVPIATVIGIGIAILIGGVVVTESVYAIPGLGRLTVDAVLA
                     RDFPTIQGVILFFSFIYVLVNLLIDLSYVFLDPRIRY"
     gene            28847..29704
                     /locus_tag="VIG70_01285"
     CDS             28847..29704
                     /locus_tag="VIG70_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8249209.1"
                     /translation="MLWRNWSVRIGGAALVLLLLVSMLAPWLGTVDPTLFDAGSRDLR
                     PGATGEIMTLEGEVLKHTFVMGSDSFGRDIYSRVLYGTRVSLVVGIAAALLSVAFGVV
                     LGLSAGYIRWLDGPLMRVMDGIMAIPAILIAIAVVALWRASLPTVIVAIAIPEIPRVT
                     RLVRALVLSIREEPYVEAAVALGTPTPKIMFGHILPNTIAPLLVQGTYICAFSILLEA
                     ILSFLGVGLPPDIPTWGNIMAEGRVQFVAYPHNVFFPGIFLAVTVLAVNILGDGLRDT
                     LDPKMAKRV"
     gene            29704..31308
                     /locus_tag="VIG70_01290"
     CDS             29704..31308
                     /locus_tag="VIG70_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249210.1"
                     /translation="MSTLEIAGLAIRLPAGADRAQAVSDVSFTVERGEIMCLVGESGS
                     GKSVIAQAIMGLLPRALPVSAGKILLESEDLLAASEARLRALRGARMSMIFQEPMTAL
                     NPVMRCGAQIEEVLSHHTRDSAATRRQKMLDLLREVALPDPERILASYPHQLSGGQRQ
                     RIMIAMALALEPALLIADEPTTALDVTTQAQILKLIVELQQRHGMGVLFITHDFGVVA
                     ELAHRVAVLREGRLVELGPKLEVLQRPRHEYTKMLIRSVPTLEPHDRPARDDAVLVLQ
                     TRNLSKTYVDRGWLGRRREVRAASEVSLEVRKGQTLGIVGESGSGKSTVARCIVRLIE
                     PSGGEIRLGGVDLARMRARALRPLRRRVQIVFQDPYRSLNPRRSVGEAIIEGPVNYGV
                     PRAKALERARRLLELVRMDPAVLDRYPHQFSGGQRQRICIARALAMEPELLVADEAVS
                     ALDVSVQAQVLALLAEIRSRLDLAMLFITHDLRVAAQVCDRVAVMQAGRIVEYGPARE
                     VFLSPQHDYTRALFAAAPGRGFAFGG"
     gene            31318..32058
                     /locus_tag="VIG70_01295"
     CDS             31318..32058
                     /locus_tag="VIG70_01295"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY8249211.1"
                     /translation="MDRLHAAAIAIPGVEVRVVERCSSTNDLLLREVPETPLLIAAEE
                     QTAGRGRRGRRWHSAPGAGITFSLGRRIARSVRELAALSLVAGVATTRALRALGAHQA
                     ALKWPNDLVVDGAKLGGILVETRSSHAVIGIGINCRSDATVEARVGRRLAYLNSPVNR
                     NEVIRRIAVALVEAIDQFEKHGLDALRAEWEAMHAYAGQKLRVRLADGRTLSGTAAGL
                     GADGSLQLHTRQGMRSVTSGRVVSARTA"
     gene            32055..32807
                     /locus_tag="VIG70_01300"
     CDS             32055..32807
                     /locus_tag="VIG70_01300"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY8249212.1"
                     /translation="MILAIDAGNSRVKWGWHDGTGWASIATVSLIEFAASSDHVNPFS
                     VTHEDPERIIISNVAGDGAHHLLINWTSIFDVEPLWLRAEAERCGVRNLYERPEQLGP
                     DRWAALIAARALHSSGPALVVNAGTATTVDMLSGDGRFLGGAILPGVELMRFVLHEHT
                     GRLPLDEGQYRDTPRKTNDAIESGCRHAQAGAVERMYRVLRDVELNPLCIVAGGAGRA
                     LVDQLSMPRRYVENLVLDGLARIALHERTLAK"
     gene            32804..33463
                     /locus_tag="VIG70_01305"
     CDS             32804..33463
                     /locus_tag="VIG70_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249213.1"
                     /translation="MRALFLLLLCANLAFFAWARYGAPVDAGDPAPISRQIEPEKLKV
                     IAPANLPAAAAAKKAAPASMSTSTIACMEWGSFTLTDAPRAQQALEPLALGARLSQRR
                     TEEVAGWWVFIPPQGSRQGALKKGLELRNLGIDEYFIVQDEGEHRWALSLGIFRSEEA
                     AQVHLAALQAQGVRSALVGARESVVPKVWLQVKGVDASLQARLKEIARQAEGSELRDC
                     R"
     gene            complement(33468..33848)
                     /locus_tag="VIG70_01310"
     CDS             complement(33468..33848)
                     /locus_tag="VIG70_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8249214.1"
                     /translation="MAATILVVDDDESIRELLRMHLAAAGYEVHAAADAIAAGYHVLR
                     SAPDLIVCDINMPHMDGFEFIAALKADTTLPRIPVIFLTSYDEGDDRGRALGAVGYLT
                     KPVRADKLLQLVAKHVPGGALPIG"
     gene            complement(33961..34965)
                     /locus_tag="VIG70_01315"
     CDS             complement(33961..34965)
                     /locus_tag="VIG70_01315"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEY8249215.1"
                     /translation="MSTQRTIRRTLLAMALVALGANGTALALNEEEPNDLFNQPQALR
                     FDATGLATITGSIENPGMFTPDVDYYSFEGNAGELIALDIDGTSGELDTVLYVFDPTG
                     KMRYIAQDTDPADMGSNFYPTGGTGTYDPSLQFRVDIAGTWKVAVVAMPARLDDFGSW
                     GTGPQDHEAHTGGTYSFFVSRPAPKPSMQYVNIDIKPGDRRDPTPINKAKGYIPVALL
                     ADSQFDPFKIDVSSLRFGRTGKEASLVWCAQHGHRVNRDGKRDRVCYFDNRKTGFTRM
                     DTIGVLKGVTLGKVAFEGKDDVKVYPQKRHKHHGHYKEHGQYRHYERHNHKHDRDDDD
                     "
BASE COUNT         5569 a        12158 c        11954 g         5604 t
ORIGIN      
        1 agcgagctcg agcagcggca ggtaaccgca ttgcaggtgc tcggcgaccg ccgcaccgtg
       61 ccgggtgaaa tccgggccga cgaagaactc cttgtgctcg aggccgatca gcgcttccga
      121 gacgccgagc gtgccgccga gatgcgattc gctgaggaag ggctcggaaa agcgcacgcg
      181 cagccgcgag ccctcgagca gcgccttcca gcggctttcg accccgaacg ccgccagtcc
      241 gaaattgccg ggatgccggc tggtgttcgg gccgcagcgg gcgagcgctt ggcgcgcaag
      301 ctcggagagc tcttggcgca gtttgggagt caaatcgatc tccttgccgc ccaggcgcag
      361 cgatacctcg tcggcgtgcg ccgccacggc agcgcacacg agcaaggcgg acaggatcgc
      421 tttcacggtc ccactacggt aatggcgccg tcgattttca gagtcacgcc ggcaatgcag
      481 cgactttcct ccgacgccag aaacacggcc gcggcggcga cgtcgtccgc gccggcgagg
      541 cgctggagcg gcgtgcgcga gcggcgtttt tcccagcccg cggcgtcgat tacgctgttc
      601 gagcccgggg tggacaccgg tccgggtgcg agcgcgttga cgcggatgcc gtgcggcgcg
      661 agctcgaccg cctgctggcg cgtgaaggtg tcgatcgccc ccttgatcga ggagtagacg
      721 caggcgttgg tgatgccgaa ggacaccgcg accgacgaaa ggttgatgat cgcgccgccg
      781 ccgcgggcaa tgaggtgcgg cgtggcggcc tgtgtgcacc agaaaacgcc cttgatgccc
      841 acgtcgagca tgcggtgaat cttgtcctcg ggcatgtcga ccagcggcgc gtaatggaac
      901 gagacggcgt tgttcaccag gatgtcgagc ccgcccgctt cggcgacgaa gcgcttcatc
      961 gtcgcgaaga ccgcggcgcg gtcggagacg tcgcattggt agggcgtgct tccgtcgatg
     1021 tcgaccctcg ccacggtggc gccttcgcgc gcgagcgcgt cggcgatcgc gcggccgatg
     1081 ccggcaccgg cgccggtcac gaacgcggct ttcccttgca gtcggcccat cggacggata
     1141 atacattccc attccgagga gggatccatg aaacgaatcg tgtttgccgg cctgctggcc
     1201 gcggcgctca gcgcgccggt gctcgcgcaa accaaggtgc agatgcaatc cgcgtttccg
     1261 cccaggggca ccttcgccga caacctggtg ttcttcgccg agcgcgtgaa ggtgatgtcg
     1321 gcgggacggc tggaaatcgt gccgctcccc accgggcaga tcgtgcccgc cttcgaggtg
     1381 ctcgacgcca cccacaagaa agtgatcgac ggcgcgcact cctcttcggc ctactgggtc
     1441 gggaagaacc gcgccgcggc gctcttcggc gtcacccccg gcggcccgtt cggcatggac
     1501 ggcttcgact acatgggctg gctgtacgac ggcggaggca tggagctgta ccgggagctg
     1561 ttccaggtcg tgctccagcg caacgtgttt ccgatcccga tgacctcggc cgggccgcag
     1621 gcgctcggct ggttcaagcg cccggtgaag agctgggccg acttcaaggg actcaagtgc
     1681 cggcagaccg ggatctcggc cgaggtgttc gtcaaatcgg gaatgacgac cgtcaacatg
     1741 gcgggcggcg agatcgtgcc ttcagcgcag cgcggcgtga tcgactgcgc cgagtgggtc
     1801 ggcccgagcg acgacatgct gatgggcttc caccaggttt ggaagcactt ctacacccag
     1861 tcgtcgcacg acctctcgct tctcgagatc atcatcaacg gcgatgtctg gaaagcgctg
     1921 acgcccgacc tgcaggcgat cgtgcaggcc ggcgccatcg aagccacgct gcgctcgcag
     1981 aaccggcgca accggctcga cgccgacgcc ctcgaggagc tgcgcaccaa gcacaacgtc
     2041 gtcgtgcacc gcacgccgcc cgacatcctg aagaacatgc tcctggcgtg ggacggcatc
     2101 gcggccgagg aggcggcgaa gaacccgttc ttcaagaaag tgtacgactc gcagcgcgcc
     2161 tacgcgtcga aggtcgtcac ctcgcgcctg gtcacggcgc cgtcgtacaa cgcgatcgcc
     2221 gggcattact ggccccagca gccggcgcag aagaagaagt agctacggct tgagctggtc
     2281 ttcgaacttg cgcaggactt tctcgcgcag ctcgggtgac gctttcacca ccggcggaaa
     2341 atcgccgagc atctcgaacg gccggcaggc gtcgatcagc gcgcgcgagt tccacgtcac
     2401 gcccttcggc aggcgcgggt cgagcgggcc gctccaggcg cggcggatga agtcgatgtc
     2461 ctcggccggg tcgcagcgcg tgcacatcgc ccagatcacc tgatcaaggt tggcgggatc
     2521 gatgtcgtcg tcgaccacga ccacgaagcg gccgaggtag gcgcccgact ggcagctcgc
     2581 ggcgaggacc gcggcctggc gcgcatggcc ggcgtacgcc tgcttgatcg agatgacgtt
     2641 gaacatgcgc gcgcagccag gctcgtgcac ccacacgccg cgcacacctg caagccccgc
     2701 gcgctcgacc tcgtcccaga tcatcccggc cttcatcacg cacttcgagt acgagaagtc
     2761 cgaaggcggc accatcgggc aggcgagccc gaggatcggg tcgttgcggt ggtagatgcg
     2821 ctcgacgcgc accaccggct gcggcgttcg gccgccggcg taatagccgg tgaactcgcc
     2881 gaacggtccc tccggcgcgg tctcgccggg atgcatcgcg ccctcgagca cgatctcggc
     2941 gtgcgccggc atcggcagcc catagagctc gctctcgatc acctcgtagg gcatgccgcg
     3001 atgcccggcg gcgtagtcgt actcggagag gccgaagcgg atctcgttgc cgccggcgag
     3061 aaagaggagc gggtcgtggc cgcagctgat gagcaccggg cagggcttgc cctgtttcca
     3121 gtacttctcg cggatctgcc ggccgtgctt gcccggcgag atccacagcc cgaggcgatc
     3181 gggtccctgc accatcgcgc gataggtgcc gcagttgacc cagtgctgct ccgggtcgcg
     3241 catgatcacc agatcgtcgg tgccgatgta gcgcccgccg tcgtgctcgt ggagcagcgg
     3301 ggccgggaac cgcaatacat ccacgtcctt ccctttgaga atattttcga ggaccggtcc
     3361 ccgcgccacg gttttcgggg ggataggctc atgggtcttc atccgatccc ggtacgcccg
     3421 cacgacgtcc aagggatggg acggcaccgg gaatccgagc aggagagcaa gccgctttgc
     3481 cgagttcgta gcgcccgaga gcgcgcgaaa acctttcggg tacccgggaa tgtcctcgaa
     3541 caggatcgcg ggcggcgact cctcgcggta gacgatctcc gcgagcgtgc ccatctcgag
     3601 cttccagtcc gcgccgcgga tgcggcgcag ctcgcccgcg cgctctaggc gctcgatcgc
     3661 ctcgcgcagg tcggcgtgcg gccgctcata ctcgttccac agtttctggg actcaatatg
     3721 tcccacgaaa cgcgtcggcg cccggcacga agttcaggtt ggtgacgccg tggttgcggt
     3781 tgacgagcgg ccgggcgaaa aggcggtgct tcatgctgcg cacttcgtgc tcgatctgga
     3841 acagcttcct gccgtcgcgc ggatcggtga tccactggaa gcgccactcc tccgcgtcgg
     3901 tctccatgtt gatcaggccg cgctccttca gccagtggta cgggctcgcg aagtcggcga
     3961 tgtagatctg gatgtggtgg ttgtcgtact cgggctgtgg ggccgcagtc tcggtgaaga
     4021 aaaggcgctg gtcgcggcca atgctcactg aagttctgtt cttgtcgaaa cgggctgggg
     4081 cgcgcatgat ctcgttgtag aagcgcgcga tgccctcggc ggtgccctgc ggcacgtcga
     4141 agtcgacgta ggcgagcgcg agctcggtgc gcccgaactc gggcgacggc gcgtggaggc
     4201 gcacccggtt cccccacgga caggtcgcct cgatggttcc ctctttttcg cgccaggaaa
     4261 acctggtctt cacgtccggg gcgatgcgct tcatctcgtt gcccgcgtgc tccaggcgct
     4321 tcttcgcctc ttccaggctc ggcaccacga acccggcggt gccgcgcagc acttcgggcg
     4381 ccttgccgcc gcggttcggc agatgcacct gcgtgcggcc gaagttcacc cacatgttct
     4441 ccaggccgct gaagatgtac gggtcgcggg tgccgcccag gccgaccacg tagaacagcg
     4501 tcgcgaagcg ctggtcgccg acgatgacgt tgaagtgctc caggtgcacg atgttgccca
     4561 ccacctgggc gtcgcggttg tactgcttga gcttctcggg tgcgttcatg gcactagttt
     4621 attcccctgc ccctaagtag ttaatcccca cacgcgttta gcgaaatcgg ggtagtcggc
     4681 cgcgatggcc cgggccaccg ggcctcgcag cagggtcaaa tcctcggcag agggcgcagg
     4741 ggtcaacccc agcttgtccg gagcatcgta ggaaaaaccc gtatcagccc gcacgtcacc
     4801 cggctggtgc acgctttcca gccggaagcg gcgtttgtcc ttctgccacg agaacagcgc
     4861 cttcccggtc accagggcag cggggtcgcc gcgcgccgag atgaactcga ccttcggcac
     4921 cagcacccgc ggggaatgct cttcgcggaa gaggatggtg tttttcacca ccgagtacat
     4981 gaaggccgag ccgaaggagc ccgggaagcg gcgcccctcg gcgcgcacca ggttgatgtt
     5041 cgcctcgccg tcgatctgcg ccccgccgag gaagaacacg tcgatcctgc cctgccccgc
     5101 gaggtcgaac agctcgcgcg agccctcggt gaacgggttg ccgcgtcgct tatgaagcat
     5161 cgagacgcgg agcggcgcgc cccgtgcctt caacagctca caagcggtcg ccggaatggc
     5221 ggaagcggcg cccaccgcga cgtggcgcgc cccgccgatc aggcgggcga tgacgcaggc
     5281 gaggagctct tctcgggaat gcaaacgtat tcgtctaggt agcgctgaaa gccttcctgc
     5341 gtgcgcgcgg cgcgcgcgta gtgcgcgagg tgcgcgtcgt cgatgtcgta cacgccgggg
     5401 atgccgagcg gccaggcgcc gcgcttcgcc ggcgcgatgg cgctgacgta ggtcgcggaa
     5461 atcacgccgg gcgcgagcag ctcgtcctcg agcatgtcgc ccggcttgag ctcttcgaac
     5521 gtgacgaagg tctgctttgc cgcatgcgcg atcgtcgcca gctcccgccg gcgcccgacc
     5581 cacacgttcc ccgcctcgtc ggcccagcgc gcgtggaaca gcgcgatgtc gggctggatc
     5641 gccgggacga gcaggatggg gtcttcttcc ggattgaatg gattgcgccc gacctgccag
     5701 tcggcccggt gcttcacgag gtcggaaccc aggaccccgc gcagcggcat gaacggcacg
     5761 cccttctcgc tcgcctgcag cgcggtgtgg accgcgggac aggtagcgtc tttcaccttg
     5821 atgagattct tttcgaccgc ttccgagaag cgcggcgcga ggcccgcctc gccgagcgac
     5881 accgcgctcg attcgacttc ggcgacgcag cccgcgccga tcaggaggtc ggcgcacagg
     5941 ccgagcaccg gaacgccgag caggcgcaga tttctttttt tctggacgat caacgcccgc
     6001 acgatcgcca tcgcgggcat cgagtagtcc ggcggcagcg ccagcagcgc gccgtccggg
     6061 atgagacgag ctaaatcctc aggctgtgcc gccaacggtg agcccctcga tcttgagcgt
     6121 cggctgcccg acgccgaccg gcacgctctg cccttccttg ccgcaggtgc cgatgcccgg
     6181 gtcgagctgc aggtcattgc cgatcatggt cactcgcgtc agggcgtcgg gcccgttgcc
     6241 gatgagcgtc gcgcccttca ccgggtgcgt gaccttgccg ttctcgatga gatacgcctc
     6301 ggcggtggag aacacgaact tgccgctggt gatgtcgacc tgcccgccgc cgaagttggt
     6361 ggcgtagatg cctttggcga cgctcttgat gatctcggcc gggacatgcg ggccggcaag
     6421 catgtaggtg ttggtcatgc gcggcatcgt gacgtgggcg aacgactcgc ggcgcgcgtt
     6481 gccggtgagc ggcatcttca tcaggcgcgc gttcaggctg tcctgcaggt agccgcgcag
     6541 cacgccgtcc tcgatcagca cgttgcgctg ggtgggcgtg ccttcgtcgt cgacgctgag
     6601 cgagccgcgc cgcccgggca tcgtgccgtc gtcgaccacg gtgacgccgg gcgaggcgac
     6661 gcgctcgccc aggcgcccgg aaaacgccga gctgcccttg cggttgaagt cgccctcgag
     6721 gccgtggccg accgcttcgt gcagcagcac cccgggccag cccggaccga gcaccacggt
     6781 catcgttccc gccggcgccg gccgcgcctc gaggttgacg agggcctgcg acaccgcctg
     6841 gcgcgcataa gcgtcgagca catcgtcggt gaaatgcgaa tagtcggtac gggcgccgcc
     6901 gcccgagctg ccgctctcgc gcctgccgtt ttgctcggcg atcacctgca ccgagagccg
     6961 caccagcgga cgcacgtcgg cggcgatggc gccgtcggcg cgagcgatca gcaccacttc
     7021 gtactcgccg ccgaggctcg ccatgacctg cgtgatgcgc gggtcgagcg cgcggcattt
     7081 cttctcgagc ttctccagca gggcgacctt ggactccgaa gcgagcgagg cgatcgggtc
     7141 gatgtcgcgg tacagcggca ccgggacctt gcgcgccgcg gccttgaccc gccgggcctg
     7201 tcccgcggaa gcaatggctc gcgtcgcttt ggctgcgtcc tgcagcgcgg agaagctgat
     7261 ctcgtccgaa taggcgaacg cggtcttctc gcccgatacc gcgcgcacgc cgacgccctg
     7321 ctcgatgttg aagctgccgg acttgacgat gccctcctcc aggctccagc cctcgaaccg
     7381 cgagtactgg aagtagaggt cggcgtagtc gaccttgcgc atagcgagcg tgccgaagac
     7441 gcgctcgagc ttctgcgtct cgaggtcgaa gggcgcgagc agcgtcgtct tggcagtttc
     7501 caggatcaat tcaatctcct tcgaatctcc ttcaatttct catggtctat ctcagccagg
     7561 accacgccct cgccctcgtc gcggctcgcg agtatctcgc cccagggatc aacgatcatg
     7621 ctgtggcccc aggtgcgccg cccgctcgcg tgcgtgccgc cctgcgccgg cgcgacgacg
     7681 taggccttgt tttcgatggc gcgcgcccgc agcagcgttt cccagtgcgc ggcgccagtc
     7741 ggcacggtaa aggcggaggg cacgaacatg acgtcgaatt cgccgagccc gcggtagagc
     7801 tcggggaagc gcacatcgta gcaaaccgat agcgccaggc gtccgaaggg gctcgcgatc
     7861 gcgagcgggc gggtgccggg ctgcagtgtg cgcgactcgt cgtagctctc ctttccgccg
     7921 ccttcaaaac ggaaaaggtg catcttgtcg tagcgcgccg cgcgcttgcc ggtgtcgtcg
     7981 tacaccaggc aggcgccgcg aatgcgcctt tcatcatcgg tggagatggg ggcggtgccg
     8041 ccgacaatcc agatcctgtt tttcttggaa acttcagaga gaaactgctg gatcgggccg
     8101 ttgccgtccg gttcgcgcag cgccaccttg tcgccctcgt ggcggccgat caggtaaaag
     8161 ttctcgggga gcgcggcgag cttcgcgccg gccgccgccg cggcggcgat caggcgctcg
     8221 gcggcggcga ggttggccgc aacctcgggg cccgagacca tctggatcgc ggcgatccgg
     8281 gcccgggcca tcagttggcc agctccgtcg gcggcggggg caggatcttg acgaccttcg
     8341 gatcgctcca gctgccggtc accgtgtagt cgaaggcgaa gatctgcccg agcggattct
     8401 tgaggatccg ctgggcgatc gccgccgcga cccccgcgac cggattgacc agcgccacgc
     8461 ccagggcggc gctatcgctc aggctcggca atacgcgcac gcgcaggtcc tgcgtctcgt
     8521 gcgcgaggtc ggcctcgccg ctcatctcta cgtcggccgc cgagccgcgc atgcggaact
     8581 cgcgcagccg gaccacgccg ctgtcgacct gcgcgttcga ggcgatgcgg tcgaactgga
     8641 atcccttgga gaacacgtcg cggaagtcga gcgtgacgcg ccgcggcagg gcctgcaggc
     8701 tcatcagccc gatcagcttg ccgagccccg gctcgatctc caggaactgc ccgttctccg
     8761 cctgcatctg cacttcgccg ctcatcgtcg ggaagtcgag cgtcgccggg tcgccctgcc
     8821 aggcgagcgc gccgcgcatg cgcgtcctgc cgcctttcac catgccgggc tggccgacgc
     8881 gcgtgagaaa gctgccggcg tcgcccgcct cgaggtcgaa ctgcacctcg ctccgcgaag
     8941 gctcggcgcg ccagatgccc gagcctgtca gcgaggcgtc ggcgttgatc atgctcgcgc
     9001 tttcgatgcg ccagtccttg ccggcgcgcc tcgcgatgag ctctacgcgg cccagcggct
     9061 tgccgcggaa ggtgaactca tcggccacca ggtcgagatc gggaagctcg ctcggccgtc
     9121 ctgtcggccg gggctgggcg cccggcgtat cggcagggat cgtgaagtgg gcgaggcggg
     9181 ctatgaccct gggctgaggg cgcgtgcgat aggacacgtc gcctgcgagc tcatcggcct
     9241 tcacgttcgc cgaccagccc gccgccgcgg tgctcgcccg cagcccgaca ttgctcagcc
     9301 tcctgccgaa ggcgtcgagc gtgccgaact tcatctcgac ggagatcgtc tccgagctgc
     9361 cgccgccatc cccgcccgcg agcggcagcc agcgatcgag atcgaacgcg gcgagcgagc
     9421 cgtacaccag cgtgccgggc cgctcgggga gccggatcgg ctgatcccgc tccgggttga
     9481 cccagaccgc ggtgcgctgc acgaccatcg tgccgtcctg gctgcgccgc tgcacctcgg
     9541 cgcgcgcgag cgcgccgagg gtgaccgaga tgcggtcgcg ctcgcctttt ggcgccggat
     9601 tgatctcgac gcgcagcggc agcggctccg cggcgctctt ggcgagcggc gccgggagcg
     9661 tgctctcgac gccgcgcagc ggcgattcga acgtgacgcg agccacgcca tcctgggcgc
     9721 gcacggtgag gaaatagtcg aaggtgcccg acaggtgctt gccgagagga tgatcgcgca
     9781 tcgaacctgc cagcgtgcgc gccgcctcga gcgccgcgtt gccgcgcgcg agcacctcga
     9841 tgccgcggcc ggcgcgcgtt ccgcccgaga tcccgacctc gccgccgaag acgcggccgc
     9901 gcacgtcgtg cagcgtgaaa cccgattcgc cgaacgcgag ccgacccgaa gccgcctcga
     9961 tcggcggcag ccacggcagg acgttcacct cgttcgccgc gaattcgtag tcgccggcga
    10021 ccctggtctt cggcagcgcg tacagcggca ggtcgagctt cagccgcagc ttgccgcgcc
    10081 ctgccgcctt gatgtcttcg gtgaagcggc ccgcggtttc gcgcagcggc gaggactgga
    10141 ggaaccgcag gaattccgcg gtcgggccgt cggcctgccc gctcacctgc acgtgtcgct
    10201 ccgggctgcg caggctcggc atggcgacgc gcacgttcga gagctgcgca ccgaggatcg
    10261 agccgctgcg cccgacgatc tccatgcggt cgcgctcgaa gttgagctcg ccggcgatgt
    10321 cttcgatgcg cggccagccg gcggcgtact cgagcacgcc cttctccacg cgcgcggtga
    10381 cctggaactg cccggtggac ggatcgacga acggaaactc ccgcaggtcg ccgcggatgc
    10441 gcacgcgcac gtcgctcgcc tcgccggcca ggatggaatt ggcgagccag gtgcgcacgt
    10501 ccccctgcag gatgctcggc aggtagcgcc cgacctgcct cccgtccgcg cgattgagaa
    10561 cgcccgagag gtcgatgctg cccggtccgt cgccgcggcg cgcgtagctg ccgtagacgt
    10621 tgccgctcgc gtgcgcgttg gcaaaggtga gcgagccgat gcgcaccgtg agcccgcccg
    10681 aggcgtcgcg ctcccattcg acctgcccgg cgagcgagtc gagcgcaagg tgggggtcgg
    10741 gaaatacgcg cggcaactcg aggaccgact tgtgcgaggc gagcgaaagc ttgcccttgt
    10801 ccaggtcggc ctccaggctg ccggagatgc cggaaaaccc gggaagctcc tcgcgcgcgc
    10861 gcagcgccag ctccgcaaag cgactgcgca cgccgacccg tgtcggcgcg tcgaacggtc
    10921 cgctccactc cagccggaaa tcggtcaggc ggccttgcgg cgcgaactgc ttgaccgcct
    10981 gtgagatctg cggcgggacc ggcagggagc cggcgagatc ccggatcgcc tccaggtcga
    11041 cgaggctcgc gccgagcgtg ccgccggcct gcggccgcca ggcgatctgg aagtcggtct
    11101 tcggaacctc ggggccgcct tccatggcga gggcgaggcc gcgcccggaa aattcgatcc
    11161 cgtccgccag ccagcgcccg gatatccgcc cgtgcaccga gacgagctcg agcgggtcca
    11221 gcacgtcgcc gaggctcgcg cgcacgtcgg cgagcgccag gtccgccgtg aattccttca
    11281 gctcgcctcg ctcgacgctc gaccagaacc gcagcgcgcc ctggccctgg cgcacgttga
    11341 acgggtaatc cacccacgcg cgccaggcgg cgagatcggt gttccctacc tgcccgaaca
    11401 cgcgggcgct ccactcgccg cgctgcagcc cgcgggcatc gaccagcgcg cgtacctcga
    11461 cgctgctgcc gagctcggcc ggcggacgcg ccgtcacccc gagcgcgagc gagaacaccg
    11521 acgcttccac ctggacgttg aggcccgaca gcaccagcgg cggggcgccg cgcagctcgt
    11581 cgcgccattc gagctcggcg ttgcgcacca cgatctggcc ctggcccgcc agccacgcgc
    11641 cgaagccgcc gcccgagggc tcgcggggct tttcgagctt caggccggcg acgaagaagt
    11701 cgcccgccgc gtcgcgccgc acgccgaggc gcagcccttc gatcgccatg ctgtgcaggc
    11761 gcagctcgcc gtgcgccagc gagcgccagg cgaccacgtt ctcgatcgac ggcagcacca
    11821 gcacctcgcg cccctgcgcg tcgtagatcc gcacgtcgga caggccgatg tgcggccgca
    11881 ggccgagcca tccggcccgg atctcgccga ccttgaccgg caggccgatg ccgcgcgaca
    11941 tggcggcgac gatgtgttcg cggtaacgtt cgatgtcggg cagcacccag aagcgcacgg
    12001 cgagcaccag cgccgcgaag gcgaaaaatc cgccccacgc gagcacttcg accgctcgca
    12061 gcagccgctt catggtggag tcacgtccag gcggccattc taatctccta atgccggaac
    12121 taacctcgca aaccgcctcg aaaatccggc tctcccggtt cgcgcaacgc gtgctttccg
    12181 cgcgtcccga gctcgcggcc gagctcgccg acccgcagcc gttcacgcgc gccgaaatgc
    12241 tcaacgcact gggaggctcg ggcgatgacg gcgaggcgca gctgaagcgc tcattgcgcc
    12301 tgcttcgcaa ccgcgtgctc ctgcgcgtca tggcgcgcga cctgtccggg cgcgccgggc
    12361 tcgacgaggt ctgtggaacc atgaccgacc tggccgaagt ggcagtttcc agaatcttga
    12421 atcaagaaaa tctcatcgtc gtcggcatgg gcaagctcgg cgggcgcgag ctcaacgtgt
    12481 cctccgacgt cgatctggtg ttcctgtacc gcgggccggc cgaagcgcag gagcgctacg
    12541 agcgcgccgg ccgcaggctg atccggctgc tgtcggagcc gaccgaggac ggtttcgtgt
    12601 tccgcgtcga catgcggctg cggccgtacg gcgattccgg gccgctcgcg tgcaacttcg
    12661 aggcgctcga gcattacctc atcacccagg gacgcgaatg ggagcgctac gcgtggctga
    12721 aagcgcggcc gctcggcgcg agcgacgagc tgcgcgagct cgacgccatc gtgcggccgt
    12781 tcgtgttccg gaagtacctc gactacggca cgttcgcggc gctgcgcgcg ctgcacgccg
    12841 aggtgcggcg cgacgtcgcg cggcgcgagc tcgccgagca cgtcaagctc gggcccggcg
    12901 gcatccgcga gatcgagttc atcgtgcagg cgcagcagct cgtccgcggc ggccgcgacc
    12961 ccgagctgcg ggtaaggtcg accgtcaggg cacttgcagt tctgagtgag aaatctcttc
    13021 tgccggaaag cgccgcgcgc gagatggccg acgcttacgt ctttctgcgc aacctcgagc
    13081 accggctgca gtacctcgac gacgcgcagc gccacgacct gcccgcggac ggagaggacc
    13141 gcgcgcgcat cgccgagatg tgcgggttcg cgacgtggga ggggttctac caggctctgc
    13201 agcgggtgcg cagcacggtc tcgcggcact tcgacgccgt cttcgcccag cccgacgtcc
    13261 ccgccgagcc ttggcccgag catccgcgcc tcgcggcgct gcgcgcgagc cagcgctatg
    13321 ccgcgctgcc ggaggaatcg cgccgccggc tcgaccgcct gatgccggcg ctcgcgcggg
    13381 cggcgcaggc gacgcccgac ccgggcgcga ccctcgcgcg cggcgccgat ctcgtcgagg
    13441 caatcgcgag ccgcgccgcg tacctcgcgc tgctcgccga gcatcccgag gcgctcgagc
    13501 gcgtcgcgcg catgatcggc tcgtcgagct gggcggcgga attcatcacc cggcacccgc
    13561 tgctgctcga cgagctgctc gacgaccggc tgctgtatgc gccgcccgaa ttggtttcct
    13621 tccagaagac cctgcgctcg cagctcgcgg ccgcgcgcga cgacaccgag cggcgcatga
    13681 acgtgctgcg cgagatgcac caggcgcagg tgttccgcct gctcgcgcag gaccttgcgg
    13741 ggctgctcac cgtcgaaaag ctcgccgacc atctctcggc gctggccgac ctgatgctcg
    13801 aagtgaccct cgagctcgtc tgggaggatc tgcggggcaa gcacctcccg gcgccgcgct
    13861 tcgcggtcgc gggctacggc aagctcggcg gcaaggagct cggctacgcc tcggacctcg
    13921 acatcatctt cctctacgac gaccccgacg agcgcgcccc tgaggtgtac gcgcgccttg
    13981 cgcagcggct caatacctgg ctcagcagcc gcacctcgtc cgggatgctg ttcgagaccg
    14041 acctgcagct tcggccgagc ggcgcctcgg gcctgctcgt gtcgtcgatc caggcattcg
    14101 agcgctacca ggaggagaac gcgtgggtct gggagcacca ggcgctcacg cgcgcacgct
    14161 attgcgccgg cgaccgccag gtgggcgcgg ccttcgagca gatccgagaa aagatcctaa
    14221 gaaagccgcg caacgcggcc gagctgcgcg cctcgatcgt cgagatgcga gccaagctgc
    14281 acgcggcgca tcccaacaag tcggggctgt tcgacgtgaa gcacgacgcc ggcggcatga
    14341 tcgacatcga gttcgcggtg caatatctgg ttctgcaatt ttcaagagag tttgcttcac
    14401 ttacggccaa tctggggaac atcgccctgc tgaagatggc ggctgaccac ggcctgatcg
    14461 acgccgggct cgccgagcgc tgccgcgacg cctaccgaga gttccgccgc atccagcacg
    14521 cgctgcgcct gaacggcgcg cagtacgcgc gggttccgca cgagagggtc tcggcggaga
    14581 gggaatccgt ccaggcgctc tggcaggcgg tgctggctac ttcagatcct tgatctcagg
    14641 ggaatcggtg tcgagctcgc cgcgctcgag cttctcgcgc gccatcgcgg cgagcgcctc
    14701 gtagcgccgg cggtagcgct cgagctgcgc ctcctccagg tcctcgaggt cgagcagcgc
    14761 gttgtgcgcg ccctgcgtgg cgcggatcag ctcgtcgagc ttgagctgca tcgcctcggt
    14821 gtcgcgggtc tgggtgttct ggatgatgaa caccatcagg aacgtgatga tggtcgtggt
    14881 cgtgttgatg acgagctgcc aggtattgct gtagccgaag agcggcccgc ccagccccca
    14941 caggacgatg actgtcactg cgaggacgaa cgtcgccggc cggccgcaga gcgcagcgat
    15001 gcggcgcgcg acacgggaaa acctgaactg gttcatttgc cgctccggaa ggtgatgtcg
    15061 ccatagaagc tgcggcggcg cgagaagtcg tcgccggggt cggtcatcac gccgacggcg
    15121 acgatgtcgt cgggctcctc gccgaacgcg cgccggtagt cctcgaccac gttgcggcgc
    15181 acgctcaccc attcgccgac gcgctgcgcg ccgctctcga ccacgaggta gcgcacgcgg
    15241 tcggtgtacg ggctgcgaat cacggtctcg gccggaacgt cgagcatcca gacgtagacg
    15301 agcgaggcgt acggcagccc gttcgccgtc agcaccatgg cgaggcgcag cttcgcgcgg
    15361 tcgtcgaagt cgagcttgtt cgcatccccg tggaacgcga gcgacagccg cgcgagcggg
    15421 ctggtcccgg acgtcatctc cagccgcgtg ccgggctcgg cattcgcccg ccagcgccat
    15481 tcgagcaccg ggtggcggct cgggtcgacg cggatcttgc gcgagaaccc cgagccgccc
    15541 tcctgtgcat cggcttcgag cacgttcgtg ccgtccacct cgacgacccg atagtcggtg
    15601 ggatgattgc cgcgccggat gagccactgc tcccacggcg ccggcgaagc tcctggcggg
    15661 acgcgcgaga agcgcgcaac ctccacgacc tctccccgct cgcgcggcag cgagcgcacc
    15721 aggtcgtcgg accagacgag cggatcgtcg ggcgtgctcg cgcatgcccc cagcagcatt
    15781 gccgccgcag tccaggaacg cttcatcccc cggaaagact agacggcatt gcggggcgtg
    15841 cgcaaggggc cgtcgacctc ggaagcgaag cgcgccagcg cctcgagcag cgcgtcacgc
    15901 ttgaggcggt acagcacgcg ctgaccctgg cgctcgcccg cgatcagacc ggcgcgctgc
    15961 agcaccgaca ggtggcgcga gatcgccggc cggctgatgc cgaagcgctc ggcgaggtcg
    16021 gcggtcgaga gcgcggtctg cgacaggaat gcgaggatgc gccggcgcgg cagcgaggcc
    16081 agggcgtaaa agacgcgctg ggggttgtta acgagcacgt taatagtatg gcgtattccg
    16141 cggccttgtg attgaatccc cccacttcca ggagaaacac gcatgtccaa aggaaaagcc
    16201 gccctgaagg cggcggcggc tgccgcgccg ctgtcgatgg ccgaccgcga cggctggatc
    16261 tggcacgacg gcaagctggt cgagtggcgc tcggccacca cccacgtgct cacgcactcg
    16321 ctgcactacg gcctcgcggt gttcgagggc gtgcgcgcct acaagaccgc gatcggcacc
    16381 gcggtcttcc gcctgaagga gcacaccgac cgcctgttct cctcggcgca catctacatg
    16441 atgaagatcc cctacacgcg cgagcgcctg atgcaggcgc agctcgacgt ggtgcaggcg
    16501 aacggccacg acgcctgcta catccggccg atcgcgttct acggctcgga gaagatgggc
    16561 gtgtcgccgg tcggggcgaa agtgcacgtg gcgatcgcgg cctggccgtg gggcgcctac
    16621 ctcgggcccg aggcgctttc caagggcatc cgggtgaaga cgtcgtccta cgcgcgccat
    16681 cacatcaacg tcaccatggc gcgcgccaag atgtcgggca cctacccgaa ctcggtgctc
    16741 gccacgctcg aagctacgat gcacggctac gacgagggcc tgctgctcga cgtcgacggc
    16801 ttcgtcgccg aaggcgccgg ggagaatatc ttcgtggtca agaacaacaa gctgtacgag
    16861 ccggagctca cctcggccct gaccggcatc acgcgcacct cggtgatcga gctcgcggca
    16921 gagctcggct atcccatcat ctccaagcga ctgacgcgcg acgacatcta tatcgccgac
    16981 gagtgcttct tcaccggcac cgccgccgag gtcacgccga tccgcgagct cgacgctcgc
    17041 gtgatcggcg agggcaaggc cgggccggtc accaaggcga tccagaaggc cttcttcgac
    17101 gtggtcaccg gaaaagacaa gaagcgcagg tcatggctga cccccgtcaa atcgaagtga
    17161 ccgagaagga cctgccgctg cactgcccga cgccgggcac cgcgctctgg gcgctgcatc
    17221 cgcgcgtatt cctcgacgtg gcgaaggaag gcgagacgct ctgcccgtac tgcgggacca
    17281 agtacgtcta caagggagcc cccgtcaaag gccattagtt gcggatcctt gtcgttgcgc
    17341 cgaactggat cggggatgcg ctcatggcgc agccgctgct cgcgcgattg aagcagtccg
    17401 gcgcgaccat cgacgtgatg gcgccggaat gggtggcgcc cgtggtgcgg cgcatgcccg
    17461 aggtcgagga cgtgatcccg gccgcgttcc gccacggccc gttgcagctc ggcgagcgct
    17521 ggcggctcgg ccgcagcttg aagcgccggg gctaccaccg ggctttcgtc ctgcccaact
    17581 cctggaagtc ggcgctggtg cctttcttcg cgggcatccc ggagcgcgtc ggctacgtcg
    17641 gcgagttgcg ctacggcctc ttgaactcgc tccacaagag cacgcaggcg ccgatgccgc
    17701 tgcactacgc ccggctcgcc ggcgaaccgg tgggggtgcc gaaactgcag gtcaccgagc
    17761 aggaaattct cgatgcccgc cggcgcttcg gcatcgaggg tgcgtacgtg gttctgtgcc
    17821 ccggagcgga gtacggaccg gcgaagcgct ggccgtactt caaggagctc gccgagaagc
    17881 tcccggcggc ggtggtgctg ggctcatcca gcgatcggag cgcggcggcg ggaatggcag
    17941 gcaagaacct cgtcggcgag acgaccctcg acgaggcgat ccggctcatc gcaggcgctg
    18001 ccgcggtggt cagcaacgac tcggggctga tgcacgtcgc cgctgcgctc ggccggccgc
    18061 aggtggccct cttcggctcg tccagccccg agcacacgcc gccggccaac gacaaggcgc
    18121 gcgtgctgtg gctgcgcctg gagtgcagcc cgtgctttga acgagaatgc ccgctgggcc
    18181 attttcgctg tatgaaggaa atttccgtcg aacaggttct cgccagcctc aacccatgac
    18241 cggcaagatc ttccccaccg cgcaggacgc ggaaaacgcc ttctacgagt cgctcgagcg
    18301 ctgcgacctg gagggcatga tggcggtgtg gtcggaggac gaagagatcg tgtgcgtgca
    18361 cccggccggc gcgcgcctca ccggccagga cgaggtgcgc gagagctggg ccaggatgtt
    18421 cgccgccggg ccgcgcgccc gcctcgcgat cgagcggcag gtcgcgatca gcggaatgat
    18481 gctcgcggtg cacagcgtgt tcgagtcgtt cacgatcccg caagccaagc ccgaggcgca
    18541 gcccgcgccg atcatcgcca ccaacgtgta cctgcgcacc gcggcgggct ggcgcatgat
    18601 cgtgcaccac gcctcgcccg cgcccgcgca gccgcaagcg gcaccccggg acgcaccgcc
    18661 aaagattctc cattagatgt ttcgttctcc ctggtggctg ccgggcggcc acctgcagac
    18721 gatttacgcc tcgctgcggc ggccgccgcg ggtcgagctc gagcgccgcc gctgggagac
    18781 gcccgacggc gatttcatcg acgtcgattt cgccggcgac gcggccgcgc cgcgccgcct
    18841 cgcgctgttc cacggtctcg aaggctgctc ggacagccac tacgcgcgcg ccatcgctgc
    18901 gcatgccgcg cgccggggct ggcgcatcgc gttgccgcac ttccgcggct gctcgggcga
    18961 gcccaaccgc aagccgcgcg cctaccattc cggggatacg caggaggtcg actggatcct
    19021 ccggaaaatc gattcacctt tcgtgatcgg catttccctc ggaggcaatg cgctcctcaa
    19081 gtggctcggc gagcgcggcg atgacgcccg ggccctggtg cagcgcgccg ccgcggtatc
    19141 ggcgccgatc gatctgccgg cggccggccg cgcgctcgac cgcggcctga atcggctctt
    19201 ctacacgcgc cacttcctgt ccacgctcag gcccaagtcg ctcgccaagc tcgagcgctt
    19261 tcccgggctc tacgacgcgg cgcgcgtgcg cgccgcgcgc cgcttccgcg aattcgacga
    19321 caccgtgacc gcgccgctgc acggttttcg cgacgtcgat cactactgga gcgaagccgc
    19381 gagcggcccg tggctcgagc gcatccgggt gccgacgctg ctgctcaatg cgcgcaacga
    19441 cccgttcctc ccggagcatg ccctcctcgc cgccgcgcga aaggccgcgc cgtgcgtggt
    19501 gctagaattt cctcgcaccg gaggccacgc cggattcgtt tccggcccgt tcccgggcca
    19561 acatggctgg ctcgccaggc gcctcctgga tttcctagat caatgaacgt ccccgctgaa
    19621 atcttccgcg cctacgacat ccggggcatc gtcgggcgca cgctgaccgc gccgctcgtg
    19681 cgcgagatcg gccgcgcgct cggcacggtg gcgcgcgagc gcggcgcgcc ggccttcgcc
    19741 gtgtgccgcg acgggcgcct ttcgggcccc gagctcgcca acgcgctgat ggaaggactt
    19801 acggcggcgg gcgccgacgc catcgacatc ggcatgggac cgacgccgct cgcctacttc
    19861 gccgcgcatc acctgggctg cggcagctgc gtcgccgtga gcggcagcca caacccgccg
    19921 gactacaacg gcctcaagat cgtcgtgggc ggcgacgccc tgtacggcga cgacatccag
    19981 gatctgcgca cgaggaccga aagcgggaaa ttcgcctcgg gcaaaggcaa gcgcagcacg
    20041 gcggacgtgc tcgacgccta tgtcgagcgc atcgcgggcg acgtgaagct cgcgcgcaag
    20101 ttccacgtcg cgatcgactg cggcaacggg gtcgccggca tgctggcgcc gcgactgtac
    20161 cgccggctgg gatgcgaggt caccgagctc ttctgcgagg tcgacggcac tttcccgaac
    20221 caccaccccg atccggcgca gccgaaaaat ctggtcgagc tgatcaggac gctgaaaacc
    20281 ggaaaggccg agctcggcct cgccttcgac ggcgacggcg accgcctggg ggtggtcacc
    20341 aaggacggcg agatcatcta cgccgaccgg cagctcatgc tgctcgcgaa ggacgtgctc
    20401 agccgcaatc cgggcgccga gatcatctac gacgtgaaga gcacgcggct gctcgcgccc
    20461 tggatcgagc gccacggcgg caagccggtg ctgtggaaga ccggacactc gctgatcaag
    20521 gcgaagctga aggagagcgg cgcactcctc gccggcgaga tgtcgggcca taccttcttc
    20581 aaggagcgct ggtacggctt cgacgatgcg ctctacggcg gcgcgcgcct gctcgaagtg
    20641 ttgtccaggg aaaaggacgc aaacccggtt ctgaaaaacc ttcccaacgc gccctcgacc
    20701 ccggagctgc actgggagct cgaagaaggc gagccgcatg ccctggtggc aaggctgcag
    20761 gagtcccagc ccttccccgg cgccgagcgc gtcgtcacca tcgacggcgt gcgcgtggag
    20821 tatgctgacg gcttcgggct cgcccgcgcc tccaacacca cgccgaccat cgtgatgcgc
    20881 ttcgaagccg acaattccgc cgcactcgag cgcatcaaga aggacttccg cgctgcgctg
    20941 cagccgctca aacccggtgc gccacttcct tattagcctc ttgttgggag gaaatacggc
    21001 catggctgcg tgccctgcgc tgctcgatcg caagatggac acgatcctcg accagcccca
    21061 atcgctgtgc gagtacacgg gcaaggtggt gctggtggtg aacaccgcca gccagtgcgg
    21121 ctatacgccg cagtacgaag ggctggaggc gctctaccgc aagtaccgcg atcgcggcct
    21181 ggtggtgctg gggttccctt cgaacgactt cggcggccag gagcccggct cgaacaagga
    21241 catcgccgcg ttttgcgtca accagtacgc gatcgatttc ccgatgttcg ccaagaccga
    21301 gctgaagaag aacccgctgt acgccgacct cgccaaggcg accggcgccg cgccgcgctg
    21361 gaacttccac aagtacctga tcgaccgcgg cggcaagcag gtgcagagct tcgacacgcg
    21421 ggtcgatccg aacgacccga agctgaccgc ggccatcgag cggctgctcg ccgctcagta
    21481 gcgagcggct gccgggctag cgggtgccgt cgaggaaggc gttgccgcgc gcgaccgcgt
    21541 ggttcagcgc atcgatcggc gacttgcccc cctgccaggt ggactcgagc tcctcgtcga
    21601 tgatgctgcg cagcttcggg aactggccga ggcgcaggct cttccagttc ggcgtgcccc
    21661 gcaggaggag ctggcgcacc gcgatctcgt gcccgggatg cgccttgtag aagcctgcct
    21721 tgcgcgtgat ctcgtaggcc gcggcgttca gcgggacgag gcccgtcctc tgatgccact
    21781 cggcctgtac ctcgggccgc gcgaagaacg cgagaaaccg cgccacgccg gcatagtcgg
    21841 cggccggccg gcccgccatg acccagaaag cggatccgct cgcgagcgtg ttcagcggcg
    21901 cgccgtcgaa gtcgtcgtag tagggaaact gcgcgatgcc gacgtcgaac ttcgcgcgcc
    21961 cgcgcagctc gtcgtagttc gccgacgagg cggcgagcag cgcgcattcg cccgcgacga
    22021 agcgcgcctc ggcctcgccg cgcccgccgg cgtaggtgaa gtagcccgac ttctgccagg
    22081 tggcgagggt cgagacccag cgcaccgcga gccggttgtt gaacatcagg cgatcggtgg
    22141 caaacgcctg gttgtgccag gccgccatgt tctcgagcag cgtcgaggca ggccaggcca
    22201 tcgtcagggc gcagtcgctg cccgaggcga gcagcgcggc gagcgtgggc accatgtcgt
    22261 accaggtggc cgggggcttg gccgggtcga gcccggcgcg gcggaacgcg tcgcggttgt
    22321 agtagagcac cggcgttgcg gatccgagcg gcagcgccag cagccggcct tcggcgtcga
    22381 ccagctcctc ggcgccgccg agcgccgcct cggcgcgcag cacggcgccg gcctcgggca
    22441 tcacctgcca cagcgggcgc gcggcatcct tctggtccag aaggaacgcg ccgccgagct
    22501 cggaggcctg cacgatatgc ggcgcgcgcg cgccgaagtt cctgcttccg cgcgcggtca
    22561 ccgcttcgac cgccgcctcg tcgtacgcgc ccttgtacgt cgcggcgacg cggaactgct
    22621 tctgcgaggc attgaagcgc gcgacgatgc gctcgaattc ggcgccgcgc gccgcgttca
    22681 tcgagtgcca cagacggatg tccgcggcca gcgccgacgc gccgcacaag agggaaacca
    22741 gcagggagaa aatccgggcg ccgatacgca acatgcggcg cgaagtatat attgggtgga
    22801 gatgcctgcc ctccacgaac gcagcgccgc cgagctcgct gccgcattcg gctgcggcga
    22861 gctctcgccg ctggaagcgg cgcgcgcggc tctcgaccgg atcgaggcgt gggagccgcg
    22921 catcaacgcc atgtaccggg tgcaccgcga agccgcgctc gagcaggcgc gcgcctccga
    22981 agcgcgctgg cgcagccgcc gcgcgctgtc cgcgctcgac ggcgttcccg tcacgatcaa
    23041 ggagaacatc tacacgcgcg gcgatccggc gccggtcggc acgcgcgcca acgaggacgc
    23101 gccgccgcag gcggccgacg ccccggcggc ggcgcggctg cgcgaggccg gctgcgtgat
    23161 cctcggcaag acgacgatgc ccgacttcgg catgctctcc tcggggcttt ccagcctgca
    23221 cggcgtgacg cgcaatccct ggcgcctgga ccgcaatcct tcgggctcga gctcgggggc
    23281 ggcggccgcc gccgtcgccg gctacgcgcc gctccacgtc ggaaccgaca tcggcggctc
    23341 ggtgcggctg ccggcgacgc attgcggcct gttcgcgctc aagccctcgc tcggccgcgt
    23401 gccggtcaat cctccctaca tgggccgcgt cgcgggcccg atgacgcgca gcgtgcacga
    23461 cgcagcgctc ctcatgagcg tcatcgcaca gccggatgca cgcgacttca tgagcctgcc
    23521 cgaccaggag ctcgacttcc cggcgctgca gccgctcgcc gccaagggcc tgcggatcgg
    23581 cttcgttccc gacatgggcg cggggctgga cgtgcatccc gaagtgcggg cggccgccga
    23641 ggcggcggcg aaggcgctcg ccggggcggg ctgcgaggtc gggtcgatcc gctcgttcct
    23701 cacgcgcgag atgctcgacg gcatgtgccg cttcttcgag gcgcgctcct acaacgacct
    23761 gatgcagctc ccgccggcga agcgcgagaa ggtgctgccg ttcgtcgccg agtggtgcag
    23821 ctggcgcggc gcgcatttct ccggccgcga cgtgatgcag gcgtatgcgc tggtcatgtc
    23881 gatgcgcgaa gccgcggtcg cggcgagcga gccttacgac ttcatcctgt cgccgacttc
    23941 gccgatcctt ccctacgagg ccgagcttcc cgcgccgggc aacgacccgt gcgatgccct
    24001 gccgcacatc gccttcacgg tgccgtacaa catgtccgag cagccggcgg cgtcgatcaa
    24061 ctggacctac agcagcgaag gtctgccgat cggcgtgcag gtggtcggca ggcggttcga
    24121 tgatgcgggg gtcttgagaa tgtcggcact gctggaaggc ttacgtcccc agcagcggcc
    24181 ctggccgcag tagcacgagg acgagcgcca gcaggcccgg aacggcctgc acccagagga
    24241 tcttgcggct cgccgtggcc gcgccgaaca cgccggcgac gatcacgcag acgaggaaga
    24301 aaaccgtcac ctgcgctccg gcggccaggc cccacagcag gccggcggcg agaaagccgt
    24361 tgtacaggcc ctggttcatc gccagcgtct tggtcgcggc cgcgtgctcg ggcgtcatgc
    24421 cgaaagtgcg ccgtccgaaa ggcttgtccc agaggaacat ctcgagcacc aggaagtaga
    24481 tgtgcagcag cgcgaccagc agtaccgaga tctccgcggc gagcttcatc agcgggattt
    24541 cttcaagcgg ggatccaggc ggtcgatatc gccctgcacc tggtcgcggc tgcgctcgta
    24601 cacgatctcg cgccccatga ttttcccggc ataggccatg ccgttgcgcg tcggtgtgag
    24661 cgtgcatttc acgttgtgca cgccttcgcc cagcaccacc tcgatcgatt cggcgcgctt
    24721 gctgaacacc accacctcga tcacctgccc ggtgtcggtg tttctgacct tgatcttctc
    24781 ggaattcatg cgtgcaccgg cccgagcggc cgcagccgca cgcccttgcg cagcttgctc
    24841 catttgaatt cggcggggtc ggcgagcgcc ggcccgggtg acttgaccac gaggacctcc
    24901 ttcgcaatgg gctcgaagtc ggcgcggaag tgcaccgagc tcttgagcgc cacgatgcgg
    24961 ctcttgcgcg gctcgacgcc gagcgtgcgg aacatttcct ggtcggcggc ctggcacttg
    25021 cgcgaggcga gcaccacgcg cacgccgggc gcggcgcggc tgcgcaagag cgccatcggg
    25081 ccgagcgtca tgcgaaagcc cttgaacatc ggcccgctgc aggtgaagcg gccgtcgccg
    25141 atccgctcga ccgtgaactc gccgctcaac ggcgcatccc ccgggatccg ggaccgcccg
    25201 cccagggaaa aggtcgggct cgacccctgc cccgcctcgt gcgcgcgctg cgccgacgct
    25261 ggatcgatca gcatgcccaa cacggcatcc gaggcgttct gatcaatgag agaacgaagc
    25321 agacccgtgg tgtcgccatt accgccggcg ccggggttgt cctgcgtgtc ggcgagcacc
    25381 accggcgcgc cgggctcgcc gacgctgcgc gcgcgccgca cggcgagcgg cgcctcatgc
    25441 agctccatcg cgaaatcctt ctccgcgtcg gcaaccgcgc cgcgcaactg ctccagggct
    25501 cccacggagc cgtagccgaa gaccgacatg ccgcagtcgg cgaagtcggc catcgggaag
    25561 ccgggggtga atgacagcac gcagccgtgc cgttcttcca acttggacag ttcctgatag
    25621 atcgtcttgc acggctcgat gaaggagcac tgcgacggga tgccggtgag gtagtcgagc
    25681 tggtggaagc cgccctgcgg gcgctggcct gcgcgcagca tctgatcgag aacgcgtgcg
    25741 gcgcgcgcgc cggtttcggc catgtcgacg tgcggatacg tccgataggc gaccagcgcg
    25801 tcggcgcgct cgaacatcgc gcgcgtcacg ttcgcgtgaa ggtcgaggct cgcgacgacc
    25861 ggcacgcgcg ggccgacagc ttcgcgcacg cgccgcagga gctcgccctc gccgtcgtcg
    25921 taggtctcca ccaccatcgc gccgtgcagg tccagataaa cgccatcgag gggcagctcg
    25981 gccctgaggc gcttcacgag ctcggtgctg attctttcga aagcatcggc cgtgacgtgc
    26041 gccgaggggc tcgccgcgcc ccacgcggtg ccgaccaggc tgtgcccgag cgagcgcagc
    26101 gcgtggatcg cgccggccgc cgggatgttc gcgccttcga ccgcggcgaa aatgggctcg
    26161 ccgtagcgca cgccgggcca gccgccgccc gcctcgaacg ccgcgtagtc ggccttggac
    26221 ggcgcaaagg tgttggtctc gtgctggaag ccgccgattg cgatgcgtgc catgccctgc
    26281 taacctgttt tctcctagga ggaggattct cgcatgcgtt atcgcgttct cgccgcagcg
    26341 ctcgtccttt ccgcatccct tggcgtcgct ccgggctttt ctcccctcca cgcccagacc
    26401 aaggtgcttc gggtggtgcc gcactcgaac ctcaacatcc tcgacccgat ctggaccacc
    26461 cagtacatgg cgcgcaacca cggctacatg gtgtacgaca cgctgttcgg caccgacgag
    26521 aagagccgca tccagccgca gatggtcgaa agatggaccg tcagcaagga ccagaaggag
    26581 tggagcttca cgctgcgcgg cggcctggag ttccacgacg ggaaaccggt gaccggcgag
    26641 gacgtgatcg cctcgctcca gcgctggggc aagcgcgacg ccatgggcca gaagctgatg
    26701 acgttcgtcg agcgcatgga ctcgcccagc ccggcgagct tccgcatcgt gctgcgcgag
    26761 ccctgcggct tcgtgctcga ggcgctgggc aagccctcct ccaacgtgcc cttcatcatg
    26821 cccaagcgcg tcgccgatac gccggcggac aagcagatcg aggacgcgat cggcagcggc
    26881 ccctacatct tcaagaaaga cgagttcaag ccgggcgaca aggcggtcta tacgagaaac
    26941 gacaaatacg tgccgcgcaa ggagacgccc tccggcaccg cgggcggcaa gcacgtgtac
    27001 gtcgaccgcg tggaatggaa cctggcgctg cgcgacgcgc agtcgcaggt gaacgcgctc
    27061 gccaacggcg aagtcgacgt gctcgagcgt cccgcgttcg agagctacga gcagctgaaa
    27121 accgcgccca acgtgcaact cgtcaccaag gacccgctcg gcttccagta catgtgccgc
    27181 ttcaaccacc tgcacccgcc gttcaacaat gcgaaagtgc gtcaggcggc gctcgcggcg
    27241 atggcgcagg agcccttcct gcgcgcgcag gtcggcatca aggagttcta ccggccgtgc
    27301 gcctcgatgt tcacctgcaa cacgccttac ggctcgagca agggcagcga gatccagtcg
    27361 aagtccagca tgaagaaagc gcaggagctg gtgaagagct ccggctacga cggcacggcg
    27421 gtggtgatca tcaagcccac cgatctcgcg gcgatccaga agctgcccga cgtcgcggcg
    27481 cagctcctgc gccaggcggg cttcaaggtc gacctgcagg cgatggactg gaacaccgtg
    27541 gtgtcgcgac gggcgaagaa agagccgccg gcgcagggcg gctggaacat cttctgcacc
    27601 gcgtgggtgg cgcccgacat ctggaacccg ctcaccaacg cggcggtcgg cgcgcagggc
    27661 gagaaatcct ggttcggctg gccgagcgac gcgcagctcg agaagctgcg cgacgagttc
    27721 gcgcgcgcga ccgacgaggg caagaagaag ggcctcgccg aggcgatcca ggcgcgcgcc
    27781 ttcgagatcg gcacgcacgc gccgctcggc gagtacgtga acccgctcgc cacccgcaag
    27841 aacatcagcg gctgggtgat cggcccgggc gacatctact ggaacgtcaa gaagaactga
    27901 cctaactggt taacttcatc gcgcggcggc tggcggcgac cgtcccggtc ctgctggtcg
    27961 tcgcgatctt cgtgttcctc ctgctgcggc tgacgccggg cgaccccgcg gcgatcatgg
    28021 cgggcgacgc cgcgaccgcg gagcagatcg aaagcatccg cgccggcctc ggcctcgacc
    28081 ggccgatcgt ggtgcagttc ggcctctgga tgcaaaagat cctgtccggc gacctcggcg
    28141 agtcgttcta cttccgcatc aaggtggcga cgctcatcgg ccagcgcctc gagccgacct
    28201 tcgcgctcgc cacgctcacc atcctcctgg ccgtggcggt atcgattccg ctcggcgtgg
    28261 tggcggcctg gcgtttcggc ggctggttcg accgcgcgct gatgggcttc tcggtgctgg
    28321 gattctcgat cccggtgttc gtgctcgcct accttctgat ctggctggtg tcgctcaagc
    28381 tcgggtggtt tcccgtccag ggctaccggc ggctggcgga cggctttttc ccgttcctgc
    28441 accacctcgt tcttccggcg gtcacgctgt cggtgatcta catcgctctc atcgccaggg
    28501 tgacgcgcgc ctcggtgctg gaagcgctcg gcgaggatta catccgcacc gcgcgcgcga
    28561 aaggcctgcc tgagctgcgc gtgctggtgc gccacgcgct cgccaacgcc gccgtgccga
    28621 tcgcgacggt gatcggcatc ggcatcgcga tcctcatcgg cggcgtggtg gtcaccgaat
    28681 cggtctacgc catccccggc ctcggccggc tcacggtaga cgcggtgctc gcgcgcgatt
    28741 ttccgaccat ccagggcgtg atcctgttct tctctttcat atatgtactg gtgaacctgc
    28801 tgatcgacct gtcgtacgta ttcctcgatc ccaggatccg gtactgatgc tctggcgcaa
    28861 ctggtcggtc cgcatcgggg gggcagccct ggtcttgctt ctcctcgttt caatgcttgc
    28921 gccatggctt gggacggtcg atccgacgct gttcgacgcc ggaagccggg acctcaggcc
    28981 cggcgccacg ggcgagatca tgacgcttga gggcgaagtc ctgaagcaca ccttcgtgat
    29041 gggctcggac tcgttcggcc gcgacatcta cagccgcgtt ctctacggca cacgcgtttc
    29101 gctcgtcgtc ggcatcgctg cggcgctgct ttcggtggcc ttcggggtcg tccttggact
    29161 gtccgcgggt tacatccgct ggctcgacgg cccgctgatg cgcgtcatgg acggcatcat
    29221 ggcgatcccg gcgatcctga tcgcgatcgc ggtggtcgcg ctctggcgcg cgagcttgcc
    29281 caccgtgatc gtggcgatcg cgattcccga gatcccgcgt gtcacgcgcc tggtgcgcgc
    29341 gctagtgctc tcgatccgcg aggagccgta cgtcgaggcc gcagtggcgc tcggcacgcc
    29401 gacgcccaaa atcatgttcg gccacatcct gccgaacacc atcgccccgc tcctcgtgca
    29461 aggcacttac atctgcgcgt tttcgatcct gctcgaggcg atcctgtctt ttctcggcgt
    29521 tggccttccg cccgacatcc cgacctgggg caacatcatg gccgaagggc gcgtgcagtt
    29581 cgtcgcctac ccgcacaacg tgttctttcc gggcatcttc ctcgccgtga cggtgctcgc
    29641 cgtcaacatc ctcggcgacg ggctgcgcga cacgctggac ccgaagatgg cgaagcgcgt
    29701 ctgatgagca cgctggagat cgccggcctg gcgatccggc tgcccgccgg cgccgaccgc
    29761 gcgcaagcgg tttcggacgt ctcgttcacg gtggagcgcg gcgagatcat gtgcctggtc
    29821 ggagagtcgg gctccggcaa gagcgtgatc gcgcaggcga tcatgggcct gcttccgaga
    29881 gcgctgccgg tttcggcggg gaagattctt ctggagagcg aggacctgct cgccgcgagc
    29941 gaagcccgcc tgcgcgcgct gcgcggcgct cgcatgtcga tgatcttcca ggagcccatg
    30001 accgcgctca acccggtgat gcgctgcggc gcgcagatcg aggaggtctt gagtcatcac
    30061 acccgggaca gcgccgcgac ccgccgtcag aaaatgctcg atctcctgcg cgaggtagcg
    30121 ctgcccgatc cggagcgcat cctcgcctcg tacccgcacc agctttccgg cggccagcgc
    30181 cagcgcatca tgatcgcgat ggcgctcgcc ctcgagccgg cgctgctcat cgccgacgag
    30241 cccaccaccg ccctcgacgt gaccacgcag gcgcaaatcc tcaagctgat cgtcgagctg
    30301 cagcagcgcc atggcatggg cgtgctgttc atcacgcacg acttcggcgt ggtggcggag
    30361 ctcgcgcacc gcgtcgcggt gctgcgcgaa ggcaggctgg tcgagctcgg ccccaagctc
    30421 gaagtgctgc agcggccgcg gcacgagtac acgaaaatgc tgatccgctc ggtgccgacg
    30481 ctcgagccgc atgatcgccc ggcccgggac gatgccgtcc tggttctaca gaccaggaat
    30541 ctttcaaaga cgtacgtcga ccgcggctgg ctcggccgcc ggcgcgaagt gcgcgccgcg
    30601 agcgaagtca gcctcgaggt gcgcaagggc cagaccctcg gcatcgtggg cgagtcgggc
    30661 tcgggcaaat cgaccgtggc gcgctgcatc gtgcgcctga tcgagccgag cggcggcgag
    30721 atccggctcg gcggcgtcga cctcgcgcgc atgcgagcgc gcgcgctgcg gccgctgcgc
    30781 cggcgcgtgc agatcgtgtt ccaggatccg taccgctcgc tcaacccgcg ccgcagcgtc
    30841 ggcgaggcga tcatcgaggg accggtcaac tacggcgtgc cgcgcgccaa agcgctcgag
    30901 cgcgcccggc gcctgctcga gctggtgcgc atggacccgg cggtgctcga tcgctacccg
    30961 caccaattca gcggcgggca gcgccagcgc atctgcatcg cgcgcgcgct cgccatggag
    31021 cccgagctgc tggtcgccga cgaggcggtc tcggcgctcg acgtctcggt gcaggcgcag
    31081 gtgctcgccc tgctcgcgga aatccgctcg cgcctggacc tggcgatgct cttcatcacg
    31141 cacgacctgc gcgtcgccgc gcaggtctgt gaccgggtcg cggtgatgca ggcggggcgc
    31201 atcgtcgaat acgggccggc gcgcgaggta ttcctctcgc cgcagcacga ctacacgcgc
    31261 gcgctgttcg ccgccgcgcc ggggcgaggc ttcgcgtttg gcggctaaac tagcaagatg
    31321 gacaggctcc atgcagcggc gatcgccatc cctggcgtag aggtgcgcgt ggtggagcga
    31381 tgcagctcca ccaacgacct gcttctcagg gaggtcccgg aaaccccgct cctgatcgcg
    31441 gccgaggagc aaactgccgg ccgcggccgg cgcggccggc gctggcacag cgcgcccggc
    31501 gcggggatca cgttctcgct tggccggcgc atcgcgcgct cggtgcgcga gctcgcggcg
    31561 ctgtcgctcg tcgccggcgt tgcgacgacg cgagcgctgc gagccctcgg cgcgcaccag
    31621 gccgccctca agtggcccaa cgacctcgtc gtcgacggcg ccaagctcgg cggcatcctg
    31681 gtcgagaccc gcagcagcca tgcggtgatc ggaatcggca tcaattgccg aagtgatgcg
    31741 acggtcgagg ccagggtcgg ccgcaggctc gcttacctga acagcccggt taaccgaaac
    31801 gaggtgatcc ggcgaatcgc ggtcgccttg gtcgaggcca tcgaccagtt cgagaagcac
    31861 ggactggacg cgctgcgcgc ggaatgggaa gcgatgcacg cgtacgcggg ccagaaactg
    31921 cgcgtgcggc tggcggacgg gcgcaccttg agcggcaccg cggctggact gggcgccgat
    31981 ggctctttgc agcttcacac gaggcaaggc atgcgctccg tcacgagcgg ccgcgtggtc
    32041 tccgcgagga cggcatgatt ctcgcgatcg atgccggcaa ctcgcgggtc aagtggggat
    32101 ggcacgatgg caccggctgg gccagcatcg ccacggtctc gctcatcgag ttcgccgcct
    32161 cgagcgacca cgtcaacccg ttctcggtga cgcacgagga ccctgagcgc atcatcatct
    32221 ccaacgtcgc cggcgacggc gcgcaccacc tcctcatcaa ctggaccagc atcttcgacg
    32281 tcgagccgct ctggctgcgc gccgaagccg agcgctgcgg cgtgcgcaat ctctacgagc
    32341 gcccggagca gctcggcccc gaccgctggg ccgcgctgat cgccgcgcgc gcgctgcact
    32401 catcgggccc ggcgctggtc gtgaacgccg gcaccgcgac caccgtcgac atgctctccg
    32461 gcgacggccg cttcctcggc ggcgcgatcc ttccgggggt cgagctgatg cgcttcgttc
    32521 tgcacgagca caccgggcgg ctgccgctcg acgaggggca gtaccgggat accccgcgca
    32581 agacgaacga tgcgatcgaa agcggctgcc gccatgcgca ggcgggcgcc gtggagcgca
    32641 tgtatcgcgt gctgcgcgat gtcgagctga acccgctgtg tatcgtggct ggtggcgccg
    32701 gccgcgcgct ggtcgatcaa ctctcgatgc cgcgccgcta cgtggagaac ctggtcctgg
    32761 acggcctggc gcgcatcgcg ctccacgagc gcacgctggc gaaatgaggg cgctgtttct
    32821 gctgctgctg tgcgcgaacc tggcgttctt tgcgtgggcg cgctacggcg cgcccgtcga
    32881 cgccggcgac ccggcgccga tctcgcgcca gatcgagccg gagaagctca aggtgatcgc
    32941 gcctgccaac ctgccggcgg ctgcggccgc gaagaaagcc gcgcccgcat ccatgtccac
    33001 ttccacgatt gcttgcatgg aatggggcag cttcacgctc acggacgcac cgcgcgcgca
    33061 gcaggcgctc gagccgctcg ccctcggcgc gcgcctctcc cagcgccgca ccgaggaggt
    33121 ggcgggctgg tgggtgttca tcccgccgca gggcagccgc cagggcgcgc tgaagaaagg
    33181 gctggagctc aggaacctcg gcatcgacga gtatttcatc gtgcaggacg aaggcgagca
    33241 ccgctgggcg ctgtcgctcg ggatattccg cagcgaggaa gcggcgcagg tgcacctcgc
    33301 ggcgctgcag gcgcagggcg tgcgcagcgc cctggtcggc gcgcgggaaa gcgtggtgcc
    33361 gaaggtctgg ctgcaggtga aaggtgtcga tgcctcgctc caggcacggc tgaaggagat
    33421 cgcccgccag gcagaaggca gcgagctgag ggattgtcgc tagagcatta accgatcggc
    33481 agggcgccgc cgggcacatg cttcgcgacc agctgcaaca gcttgtcggc acgtaccggc
    33541 ttggtgagat agcccaccgc gccgagcgcc ctgccgcggt cgtccccctc gtcgtacgag
    33601 gtgagaaaaa tcaccgggat gcgcggcagc gtggtatcgg ccttcagcgc cgcgatgaac
    33661 tcgaagccgt ccatgtgcgg catgttgatg tcgcagacga tcaggtccgg ggcgctgcgc
    33721 agcacgtggt accccgcggc gatcgcgtcc gcggccgcgt gcacctcgta gccggccgcc
    33781 gccaggtgca ttcgaagcag ctcgcggatg ctttcgtcgt cgtcgacgac gaggatcgtt
    33841 gccgccatgg attggcaaag attatagggc ggaatgattt ctgcatgatc ctcaggccca
    33901 tggggaaaca aaaaggcccg gggtctccgg gccttttcga catctggatt ttcagccaga
    33961 ttaatcgtcg tcgtctctgt cgtgcttgtg gttatggcgt tcgtagtgtc tgtattgccc
    34021 gtgctctttg tagtggccgt ggtgcttgtg gcgtttctgc ggatagactt tcacgtcgtc
    34081 tttgccctcg aacgccactt tgccgagcgt cacacccttc aggacgccaa tggtgtccat
    34141 tcgggtaaac ccggtcttcc ggttgtcgaa gtagcacacg cggtcgcgct tgccatcgcg
    34201 gttgacacga tggccgtgct gcgcgcacca gacgaggctt gcctctttgc cggtacgtcc
    34261 gaacctgaga ctggagacgt cgatcttgaa tgggtcgaac tgactgtccg ccagcagcgc
    34321 caccggaatg tagcctttcg ccttgttgat cggcgtgggg tcgcggcggt cacccggctt
    34381 gatgtcgatg ttgacgtact gcatcgacgg cttcggcgcc gggcgcgaga cgaagaagct
    34441 atacgtgccg ccggtgtgag cttcgtgatc ctgcggtcct gtcccccagg aaccaaagtc
    34501 gtccaggcgg gccggcattg cgacaaccgc gaccttccag gtgcccgcta tgtccacgcg
    34561 gaattgaaga cttgggtcgt acgtcccggt tcctccggtc gggtaaaagt tggatcccat
    34621 atccgcggga tccgtatcct gggcgatata cctcatcttg cccgtaggat cgaaaacgta
    34681 gaggaccgta tcgagctccc cagacgtccc gtcgatatcg agggcaatga gttcgcctgc
    34741 gttcccctcg aacgagtagt agtcgacgtc gggtgtaaac atgccgggat tctcgatcga
    34801 gcccgtgatc gtagccaacc ctgtggcgtc gaatcggaga gcctggggct ggttgaagag
    34861 atcgttgggc tcctcctcgt tgagggccag cgcggttcca tttgccccca gcgcaaccaa
    34921 cgccattgcc aacagcgtgc ggcgaatcgt gcgttgcgta ctcatttgct cactcctctt
    34981 agttatcgtc acggtcaccg gctttcgtcg gtgactccct gtcgtgcgtg gacgattagg
    35041 cgcgtcgcgc cgccggggtg tcggtgaaca agtgcgcaca cttgctgacc tcacaacctc
    35101 ccgaaaggcc gtttgtcgca cgttgtcgtc gccgtggtga tagacgtcac gcgcggcgac
    35161 gaactttgtc atgcgacaaa aactgtcaga caaaaactgt cagccccacg gcgagcacac
    35221 gttcgctcaa gcgaaaaagc tttgggaagt agaaggaggt atgcgccgcg cgccttcgca
    35281 acaac
//
LOCUS       DATBSZ010000020         4138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_207157, whole genome shotgun sequence.
ACCESSION   DATBSZ010000020 DATBSZ010000000
VERSION     DATBSZ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207157"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VIG70_01320"
     CDS             complement(<1..692)
                     /locus_tag="VIG70_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8249216.1"
                     /translation="MFEPMAELLAARFRITSIDQRGHGRAAKPAIGYAAEDYARDIND
                     RLSEKTLLIGHSLGARNALVTGASYPDKVAAVVAIDFTPYIEPEVFDALEARVEAGGR
                     DFASLEEIRKYLRERYPRLPADAIERRARHGYENVNDKWRPLAAPAAMQETCLGLRQD
                     LSSTLKEIRVPVLLLRGEHSKLVSRHAWTKTRALRPDIAAQEIAGADHYVPEEQPAAV
                     ASAVLDFWDAIQ"
     gene            complement(736..1479)
                     /locus_tag="VIG70_01325"
     CDS             complement(736..1479)
                     /locus_tag="VIG70_01325"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEY8249217.1"
                     /translation="MNVTKLKPKRENATTPARELPQHLIADADRPEHRYLGSELLLYL
                     TSGKLPKEALKDYAVLRWAFQAHANPAMMLSHAAFLQGEDVAHLLENAFDEIFRLAGE
                     GDHPGLWVQFAKLLGATDAELDEVAARPLAEVIGFPRTMTHYCRKSAAEGLATWWGDE
                     KQLPEAHGATALALTKYYGLSDETVEYFYEHVRADIEHTEHSFSLFEGYVKDRGDVVR
                     GRRAAAVTLWAWREMHDGILRYLRQKYDF"
     gene            complement(1476..2390)
                     /locus_tag="VIG70_01330"
     CDS             complement(1476..2390)
                     /locus_tag="VIG70_01330"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8249218.1"
                     /translation="MKMATATINDVQHEWLKRFDAAMKPQAARLKIEIYPASQLGQIP
                     RMVEGVAVGTIESFVTPTSFLVSVEPRYQVFDAIGVFGSPAHLNRVLQDPVLRKRALA
                     IGEEKGLKGIGIFYNSPIIVLARRPIERLADFNGKKIRVFGTPLQIEPMKKLGASPVP
                     MAFGEVIAALQNGTVDGMLAGMPVLTPMKFYDVAKNVTEIHPSIVTSIQVVNKKWFEG
                     LPAEVQAAMLAVGERVDKEAFSFATALVDKANKDWQANGGQLLRLPEAEQAKLMADLK
                     AIGTQLLSANPAIKTEYDELLKVAERTR"
     gene            complement(2468..3340)
                     /locus_tag="VIG70_01335"
     CDS             complement(2468..3340)
                     /locus_tag="VIG70_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8249219.1"
                     /translation="MKGTLIGVGTMGPGIAATLARGGLEVTCFDVSAEQLRKAEAEMR
                     VLQVKATIEPDLARAVAGADLIVEAVPERLDLKQQVFRDLEKLVPPTTILASNTSGIP
                     ITRLQQAVEHQGRVVGMHWSNPPHIIPVVEVIAGAHTDPEVLEKSRKIITDCGLMPVV
                     VKKDVPGFVHNRLLYAILREAVSLVEKGVVSPEELDKCFKWGLGLKLSVIGPMELLDV
                     AGLDIYEAVAGYLNADLENRTSVAPYVTRLTREKHLGMKTGKGIFEYTPEGAQQLRAA
                     RGAKLLAVRKAMQS"
     gene            complement(3337..4107)
                     /locus_tag="VIG70_01340"
     CDS             complement(3337..4107)
                     /locus_tag="VIG70_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY8249220.1"
                     /translation="MASERAGRNMPIMAKNDDTRRGLWDLSRAHRILDLEGHNDMSLG
                     HCSMRDPWERGVWLKRGNIGLEEVREDDFILLDFEGNILAGEGVRHLEWPIHTEVLRA
                     RPDLNFVCHTHPHWATLFSCTREKLMPLTNEGCWFTAPPPHYLTTSDLIDTPQLGKDL
                     AESLGRGEAAFLRNHGILSVGRTVKEMTLCAIFLEKACRAQLRLGASGFKHSPPPAEE
                     VEKKRKNIYPKRAVDNFWDYYNRRLDRVEGNKYEGEVR"
BASE COUNT          639 a         1350 c         1356 g          793 t
ORIGIN      
        1 cattggatgg cgtcccagaa atcgagcact gcggaagcga cggcggccgg ctgctcttcc
       61 ggcacgtagt gatcggcgcc ggcgatctcc tgcgccgcga tgtccggccg aagggcgcgc
      121 gtcttggtcc acgcgtggcg cgacacgagc ttgctgtgct cgcctcgcaa cagcaggacc
      181 ggcacgcgga tctctttcaa cgtggaggaa agatcctggc gcaggcccaa gcaggtttcc
      241 tgcatggccg caggcgccgc cagcggccgc catttgtcat tcacgttttc atacccgtgg
      301 cgcgcgcggc gctcgatcgc atcggccggc agacgcggat agcgctccct caggtatttc
      361 cggatctcct cgagcgacgc gaagtcgcgt cctccggcct cgacccttgc ctcgagagcg
      421 tcgaatacct cgggctcgat gtagggcgtg aagtcgatcg ctacgactgc agcaaccttg
      481 tccggatagc tcgcgcctgt cacgagggcg tttcgcgcgc cgagagagtg gccgatcaac
      541 aaggtttttt cagaaagtct gtcattgata tcgcgagcat agtcctcggc cgcatagcca
      601 atcgccggct tcgcggcgcg gccgtgcccg cgctggtcga tgctggtgat gcgaaagcgc
      661 gcggcgagga gctcggccat cggctcgaac acgtgcgcgt tcgccgtgat gccatgcagg
      721 cacagggcgg cgggcctaga agtcgtactt ctgtctcaga taacgaagga tgccgtcgtg
      781 catctcgcgc cacgcccaga gcgtgaccgc ggccgcgcgc cggccgcgca cgacgtcgcc
      841 gcggtccttg acgtagccct cgaacaggct gaacgagtgc tcggtgtgct cgatgtcggc
      901 gcgcacgtgc tcgtagaagt actcgacggt ttcgtccgag agcccgtagt acttggtcag
      961 cgcgagcgcg gtggcgccgt gcgcctcggg cagctgcttc tcgtcgcccc accaggtcgc
     1021 gaggccttcg gccgcgctct tgcggcagta gtgcgtcatg gtgcgcggga agccgatcac
     1081 ctcggcaagg ggcctggccg ccacctcgtc gagctcggcg tcggtcgcgc cgaggagctt
     1141 ggcgaactgc acccagaggc ccgggtggtc gccctcgccc gccagccgga agatctcgtc
     1201 gaacgcgttc tcgagcaggt gcgcgacgtc ctcgccttgg aggaacgcgg catgcgacag
     1261 catcatcgcc gggttggcgt gcgcctggaa cgcccagcgc agcacggcgt agtccttgag
     1321 cgcttccttc gggagcttgc cgctcgtcag atagagcagc agctcggagc cgagatagcg
     1381 gtgctcgggg cggtccgcgt cggcaatgag gtgctgcggg agctcgcgcg cgggggttgt
     1441 ggcgttttcc ctcttgggct ttagtttggt gacgttcatc tggtccgctc ggcgaccttc
     1501 agaagctcgt cgtactcggt cttgatggcc ggattggccg aaagcagttg cgtgccgatc
     1561 gccttcaggt cggccatcag cttcgcctgc tccgcctcgg gaagcctgag gagctggccg
     1621 ccgttcgcct gccagtcctt gttcgccttg tcgacaagcg cggtggcgaa cgagaaggct
     1681 tccttgtcga cgcgctcgcc cacggcaagc atggcggcct ggacttccgc cggcaggcct
     1741 tcgaaccatt tcttgttgac cacctggatc gacgtgacga tcgagggatg gatctcggtc
     1801 acgttcttcg ccacgtcgta gaacttcatc ggcgtgagca ccggcatccc ggcgagcatg
     1861 ccatcgacgg tgccgttctg cagcgccgcg atgacctcgc cgaaggccat cggcaccggc
     1921 gatgcgccga gcttcttcat cggctcgatc tgcagcggcg tgccgaaaac gcggatcttc
     1981 ttgccgttga agtcggcgag ccgctcgatc ggccggcgcg cgagcacgat gatcgggctg
     2041 ttgtagaaga tgccgatgcc cttcaggcct ttttcctcgc cgatggcgag cgcgcgcttg
     2101 cgcagtaccg ggtcctgcag cacgcggttc aggtgcgcgg gcgagccgaa cacgccgatg
     2161 gcgtcgaaca cctggtagcg cggctcgacg ctcaccagga acgaggtcgg cgtaacgaac
     2221 gactcgatcg tgccgaccgc cacgccctcc accatgcgcg ggatttgccc gagctggctc
     2281 gcgggataga tctcgatctt gagccgcgcc gcctgcggct tcatcgccgc gtcgaagcgc
     2341 ttcagccact cgtgctgcac gtcgttgatg gtcgcggtcg ccattttcat cgtggacgtc
     2401 tgggcgatcg cggcagccga gaacaagatc aatggaatcg aaagccaacg catgaaagtc
     2461 ccctccctca agattgcatc gcttttctca ccgccagcag cttggcgccg cgcgccgcgc
     2521 gaagctgctg agcgccttcc ggcgtgtact cgaaaatgcc tttgccggtt ttcattccga
     2581 gatgcttttc tctcgtcagc cgcgtcacgt acggcgccac gctggtccgg ttctcgaggt
     2641 cggcattcag gtagcccgcc accgcctcgt agatgtcgag gccggcgacg tcgagcagct
     2701 ccatcggccc gatcaccgag agcttgaggc cgaggcccca cttgaagcat ttgtcgagct
     2761 cctcgggcga gaccacgccc ttctccacca gcgacaccgc ctcgcgcagg atggcgtaga
     2821 gcaggcggtt gtggacgaat ccgggcacgt ccttcttcac caccaccggc atcaggccgc
     2881 agtcggtgat gatttttctt gatttttcga gaacctcggg atcggtgtgc gcgccggcga
     2941 tcacttcgac cacaggaatg atgtgcggcg gattggacca atgcatgccg accacgcggc
     3001 cctggtgctc caccgcctgc tggagccggg tgatcgggat gcccgacgtg ttgctggcga
     3061 gaatcgtcgt cggcgggacc agcttttcca ggtctctgaa tacctgctgc ttcagatcga
     3121 ggcgttccgg caccgcctcg acgatgaggt cggcccccgc cacggcgcgc gcgaggtcgg
     3181 gctcgatggt ggctttcacc tgcagcaccc gcatttcggc ctcggccttg cgcagctgct
     3241 ctgcgctgac atcgaagcac gtcacctcga ggccgccgcg cgccagcgtc gccgcgatcc
     3301 cggggcccat cgtgccgacg ccgatcagcg tgcctttcat cgcacttcac cctcgtattt
     3361 gtttccttcg acacgatcga gacggcggtt gtagtagtcc cagaaattgt cgaccgcgcg
     3421 cttggggtag atgttcttgc gcttcttctc cacctcttcc gccggcggcg ggctgtgctt
     3481 gaagccgctc gcgccgagcc tgagctgcgc gcggcaggcc ttctcgagaa agatggcgca
     3541 cagcgtcatc tccttcaccg tgcggccgac cgacaggatg ccgtggttgc gcaggaacgc
     3601 ggcctcgccg cggccgaggg actctgcgag atcttttccg agctgcggcg tgtcgatgag
     3661 atcgctcgtc gtaaggtagt gaggcggggg cgcggtgaac cagcagcctt cgttggtgag
     3721 cggcatgagc ttctcgcgag tgcacgagaa tagcgtcgcc caatgcggat gcgtgtggca
     3781 gacgaagttc aggtccggac gcgcgcgcag cacttcggtg tggatcggcc attcgaggtg
     3841 gcgcacgccc tcgccggcga ggatgttgcc ctcgaagtcg agcaggatga agtcgtcctc
     3901 gcgcacctct tcgaggccga tgttgccgcg cttcagccaa acgccgcgct cccacgggtc
     3961 gcgcatcgag caatggccga gcgacatgtc gttgtggccc tcgaggtcga gaatgcggtg
     4021 cgcgcgcgac aggtcccaca ggccgcgccg cgtgtcgtcg ttcttcgcca tgatgggcat
     4081 gttccgtccc gcgcgttcgc tagccatgag atctatttta ggcggaccca gcgatcga
//
LOCUS       DATBSZ010000021        11121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_548861, whole genome shotgun sequence.
ACCESSION   DATBSZ010000021 DATBSZ010000000
VERSION     DATBSZ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11121
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548861"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            574..1563
                     /locus_tag="VIG70_01345"
     CDS             574..1563
                     /locus_tag="VIG70_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HEY8249221.1"
                     /translation="MERKDIGDDAAKPRLLEQLRDAINRLHYSARTEERYVHWVKRFI
                     YFSGKRHPAELGAAEVTAFLNYLARERDVAAATQNQALSALLFLYREVLRQPLPWLDE
                     LDRARRPVRVPTVLTSEEVRRLLAAMRGTKWLMASLLYGAGLRLRECLRLRVKDIDFG
                     YRQILVRDGKGAKDRLTMLPSVVIEPLQAHLRRAKELHQRDVAEGFGDVELPHALARK
                     YPRAQYEWGWKFAFPSHKLSVDPRTGVVRRHHVYENYVIRGVARAARAAGIAKHVSCH
                     TLRHSFATHLLEAGYDIRTVQELLGHADVSTTMIYTHVLNKGGAGVTSPLDRG"
     gene            complement(1560..2024)
                     /locus_tag="VIG70_01350"
     CDS             complement(1560..2024)
                     /locus_tag="VIG70_01350"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY8249222.1"
                     /translation="MNLELQLAVEQLHARYAHALDAGRLEEWPAFFTEKGRYRIATAE
                     NEERGLPLPVLYAEGGAMLRDRVASLRHANIYEPQRYRHLASSFLVQRVDDRVVSSTS
                     NFVVIRVMQDGATLVFASGRYLDRIVVADMKYEERVVVCDSRRFDTLLAIPL"
     gene            complement(2024..3247)
                     /locus_tag="VIG70_01355"
     CDS             complement(2024..3247)
                     /locus_tag="VIG70_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY8249223.1"
                     /translation="MLERSWPAEGATRVPYWVYQDEAIYREEQAKIFRGDTWSYLCLE
                     AELPEPKSFITTFVGDMPVVVTRDPKGALHAWENRCAHRGALVCMQPRGAAERFTCIY
                     HNWTYDHAGNLTSVAFRRGIAGKGGMPADARPESQAPKQLRVESVHGLVFGTLSKDVS
                     SLEAYIGPEILPRMKRVLVKPLKVLGAYTQVLPNNWKLYMDNVKDTYHASLLHLFFTR
                     FRINRLTQKGGVFVSPDGAHHCTYTEMVEDRGDAYEKDGMRSAGSQEFRLEDPEVLDQ
                     VDELGDGVSIQILSLFPGFVLHQIRNSLAARQVVPKGIDRTELHWTLFGFADDDEAMT
                     RRRLLQANLVGPAGYISMEDGAATGFVQRGVAGSPERASVIEMGGATIGSGDSRITET
                     AVRGLWKAWRQHMQI"
     gene            3268..4218
                     /locus_tag="VIG70_01360"
     CDS             3268..4218
                     /locus_tag="VIG70_01360"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249224.1"
                     /translation="MRAAFLGMVLLATAASAQDWPTRTVRIVVPFPAGGSADLLPRVI
                     GESLQKQWGQPVIVDNRPGAAGNIGAAAVFQAEPDGYTLLSAPPPPLVINRLLYPKLA
                     YDSTQFVPMTVIAAIPNVLLVHPKVGVASVAELIALAKANPGKLNYASQGSGATSHLT
                     TELFKSMAGGLSITHVPYKGTAPALADLLAGQVDLMFDNLGVSLPHVKSGKLRALAVA
                     STRRFRSLPDVPALAETLPGFESVAWFGIVGPPKTPAAIAEKVAAGTAEALRNPEVLR
                     RLSDLSADPLGMTPAETAAFMKQETERWGAVIRSAGVKLE"
     gene            complement(4261..5370)
                     /locus_tag="VIG70_01365"
     CDS             complement(4261..5370)
                     /locus_tag="VIG70_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY8249225.1"
                     /translation="MSEKVLAAVRTGPSRTEFREYPMPEIPEDSALLKMEVAGICGTD
                     VKLYKTPPSSAPVIMGHENIGVIAKAGREFVRRKGFKEGDLVFVEHYVMCGTCEWCHA
                     GQYRHCENTDWRTNPEGIRYGYTSAERAPHLWGGFAQYLYLPWNAVVHRVPQGVSAEL
                     AGLVTPMANGVEWSLFDGGVGYDSAVLIQGPGQQGLSQTVICKQAGASLIVVTGTAKD
                     GKRLETAKALGADHTIDVNAEDPLARIREITGGKGVDVVLDCTAGAGTIPILLGVEAL
                     KRKAGTIVVQGELREFPNFPLERVTTKAITIKSARGHSYKACELALAQLASKRFPLEK
                     VTTHRFALKDVDLAIKSVGGMGLPSVIHASLMPWL"
     gene            5386..5652
                     /locus_tag="VIG70_01370"
     CDS             5386..5652
                     /locus_tag="VIG70_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249226.1"
                     /translation="MERADAASQVKAWTRSHFGDVTVLVTELESALPGFPPLHTVVAF
                     WTAERRHYHFKVFKPLEQVAEEDVPPAWYRDALAVTPGTDCGCC"
     gene            5685..6395
                     /locus_tag="VIG70_01375"
     CDS             5685..6395
                     /locus_tag="VIG70_01375"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY8249227.1"
                     /translation="MKCRWFVALAVVAGFGIHACAQLPSDLAQLGGWTTLIDGEKGLE
                     NWTRVGDANWRPEDGAIVAYRGKGGFLVSKGSYKDFQLRAEFWADPTTNSGVFIRVSN
                     PKRIGADSAYEVNIFDQPPDPAYGTGAIVNFAKVSPMPKAGGKWNTLEITARGQQLTV
                     LLNGVQTVELKHARFAQGPVALQYGLGARNAEGGPIKWRRVQVKPIEEKEPAKAAAAK
                     PSSGGGAASSGGGSAPAR"
     gene            complement(6402..7913)
                     /locus_tag="VIG70_01380"
     CDS             complement(6402..7913)
                     /locus_tag="VIG70_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249228.1"
                     /translation="MKILPALLSLGIAACATQDPHEGHGAHVRLGKVHFAVECNAAAQ
                     RDFDRALAYYHSFAWTQIREPLEGVLQADPGCGMAHWARALASLDNPFAWPGIISPAA
                     LSQGPGILETARRTGLKSQRERDYVDALAVFFRDADKRDHRSRAKALEAAMEQVMQRY
                     PQDKEAATLYALVLSANFDPADRKYTNQLKAAAILEPIFKEQPQHPGVAHYLIHSYDY
                     PPIASQGLDAARRYSRIAPDAAHALHMPSHIFTRVGAWKESIESNRESARVAREAAQG
                     ANAARGLHDVLHAMDYLVYAHLQLGQHREARAVIDQMIDTKGFNPNIRTGPYAIAAST
                     ARYAVERNDWKAAAELEVRPTRFAYVDAITHFARALGAARSGNPETARADIAKLAELR
                     DKLRSAKDAYWAEQVEIQWLVATAWLLHAEGKHDEALSTLGRAADAEDRTEKSVVTPG
                     PLAPARELYGAMLLERGMARDALAAFEATLVKEPNRYNAIAGAAKASTLLARN"
     gene            8000..8938
                     /locus_tag="VIG70_01385"
     CDS             8000..8938
                     /locus_tag="VIG70_01385"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8249229.1"
                     /translation="MDLRQLRTFVTVAEQGTVSKAALRLRIAQPALSRQISHLEDELG
                     VKLFDRVRRRLVLTGEGEQLLGDCRTVLGAVGSLSERAQLLRRGDSGVLRVAATPQMI
                     DGVFSNFLHRYAERRPKVQIKLTEAAGSGLLGMLERGELHVCITTAQVLQPDKNAFDT
                     FALPPIEFLAASHDSLKGGASGNVDIARLAAYPLLLLESTFFVRTAFDAACRLAGFKP
                     NVFIESRTPHTLLSLAEAGHGVAIVPSVLPTHRYRLRIARITHRRKPLRQPLDVVWDR
                     RRLLPPYAKDFCEALAAHMRQVFPISRPGVRARAAR"
     gene            8996..9610
                     /locus_tag="VIG70_01390"
     CDS             8996..9610
                     /locus_tag="VIG70_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY8249230.1"
                     /translation="MKRTLLAGLALGFTIIGCSQMPSWVPGSGWTTLIDGDKGMENFD
                     RVGDANWRAEGGAIVADKGKGGFLVSKNSYKDFDLRAEFWADNNTNSGIFMRCADRKT
                     ITDKSCYEANIFDQRPDQTFATGGIVHFAKVLAPAKAGGKWNTYEISARGSKVTVTLN
                     GTKTAEMDHSQLASGPVALQFGSLPPKGDPGGSIKWRKVQIRPL"
     gene            9630..10241
                     /locus_tag="VIG70_01395"
     CDS             9630..10241
                     /locus_tag="VIG70_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY8249231.1"
                     /translation="MKRLLVIATGLLVAACSSLPAWVPGSGWTTLIDGEKGLENFTRE
                     GDANWRAEGGAIVADKGKGGHLVTKSSYKDFQLRAEFWADHHTNSGIFMRIQDPKKIG
                     ANSAYEVNIYDQRAGPEFGTAGIVDFAKVPPHKYKAGGKWNTFEITAKGSQLTVVFNG
                     DKTVDIQNAKFAQGPFSLQFGNLPPKNAPGGAIRWRKVQVREL"
     gene            10256..10912
                     /locus_tag="VIG70_01400"
     CDS             10256..10912
                     /locus_tag="VIG70_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249232.1"
                     /translation="MFIGHFAVAFAAKRAAPEVSLGTLFLAAQLADLVWPTLVLLGLE
                     RFEIRPGITAVTPLDFVHYPYSHSLVALAGWAFALGFLYYAFRRGGRGAAILFAVVLS
                     HWLLDFATHRPDMPLTIGGEERFGLGLWHSRMATLAVEGLLFGACVWLYARTTRALDR
                     TGRWSFVAFVAFLMVVYVANVFGPPPPSVAAVAWSAQAIWLLVAWGYWIDRHRAASRN
                     "
     gene            complement(10888..>11121)
                     /locus_tag="VIG70_01405"
     CDS             complement(10888..>11121)
                     /locus_tag="VIG70_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8249233.1"
                     /translation="RDALFDRDTSPGAVPEDLLKLDIPALVVPGRDESHATSAARYLE
                     ECLPKSQYWDALPEAQTDAAAAPRLIQFLDAAR"
BASE COUNT         1785 a         3628 c         3839 g         1869 t
ORIGIN      
        1 ccgtgagctc catggctatg agaagggcgg agattagcca cttccaccac gtgtattcct
       61 caatcctctg aggatagatt atccagaggc accaagatcc aagtgtccaa acaagtaacg
      121 cgatccgacc ccagttacga cgccgcgatg cttggaaaat gaacaatgcc gttactgcgg
      181 tcgaagtcaa cgtaaccgta gtccaaagat acttgaactg tgaatctgcg ctcgcctcgg
      241 catcgccgta gatttgccag aaggagttca acgattcgct ggcaaggacc aaccacgaga
      301 tgatgattgc gattcttacc tcaaggggcg cagggttcgg cccatcgtgg ggtaggtccg
      361 ctactctggc tgagggagct cgataaggat cggtagtcat gcaggctacg gcccgcgccc
      421 tgtgcgctct aacgtgaatt aggtgacaaa ttagttgaca gcagtggcga tttgcaaacc
      481 gccaagatgt aacgcgtcat gtcatccctc cgtgtcctca atacgttacg ccccggtagt
      541 gcggcaagca aatatgacac tgggggagag gagatggagc gaaaggacat aggcgatgac
      601 gctgcgaagc cgcgactact ggagcagttg cgcgacgcga tcaacaggct gcattacagc
      661 gcacgaaccg aggagaggta cgttcattgg gtcaagcgct ttatctattt ttccggcaag
      721 cggcatccgg cggagcttgg agccgcggag gtcacggcat tcctgaacta cctggctcgc
      781 gagcgagacg tcgccgcggc cacgcagaac caggcgctgt cggcgctttt gttcctgtat
      841 agggaggtgc tgcgccagcc gctgccttgg ctggacgagc tcgaccgcgc gaggcgcccg
      901 gtgcgtgtgc cgacggtcct gacgagcgag gaggtgcgcc ggctcctcgc cgccatgcgc
      961 gggacgaaat ggttgatggc aagcttgctc tacggcgcgg gcctgcgctt gcgggaatgc
     1021 ctcaggctgc gcgtgaagga catcgatttc ggctaccggc agattctcgt gcgcgacggc
     1081 aaaggcgcga aggaccggct cacgatgctg ccttcggtgg tgatcgagcc gctccaggct
     1141 catttgcgcc gcgcgaagga gttgcaccag cgcgacgtgg cggagggttt tggcgacgtc
     1201 gagctgccgc atgcgctggc gcgcaagtac ccgagggcgc aatacgagtg gggctggaag
     1261 ttcgcgtttc cgtcccacaa gctgtcggtc gatccgcgca cgggggtggt gcggcgccac
     1321 cacgtgtacg agaactacgt gatccgcggc gtggcgaggg cggcgcgcgc cgcggggatt
     1381 gccaagcatg tcagctgcca cacgctcagg cattccttcg ccacgcatct gctggaggcg
     1441 ggatacgaca tccgcacggt gcaggagctg ctcgggcacg ccgacgtctc cacgaccatg
     1501 atctacaccc acgtgctcaa caagggcggc gcgggcgtga cgagcccgct cgaccgcggc
     1561 tagagcggga tggcgaggag ggtgtcgaag cggcggctgt cgcagacgac gacgcgctct
     1621 tcgtacttca tgtctgcgac gacgatgcgg tcgaggtagc ggccgctcgc gaacaccagg
     1681 gtggcgccgt cctgcatgac gcggatcacg acgaagttcg aggttgagct cacgacccgg
     1741 tcgtcgacgc gttgcacgag aaaggaagaa gcgaggtgcc ggtagcgctg cggctcgtag
     1801 atgttggcgt ggcggagcga cgcgacgcgg tcgcgcagca tggcgccgcc ctcggcgtag
     1861 aggacgggga ggggaaggcc gcgctcctcg ttctccgcgg tggcgatgcg gtagcggccc
     1921 ttctcggtga agaacgccgg ccactcctcg agccggccgg cgtcgagcgc gtgcgcgtat
     1981 cgggcgtgaa gctgctcgac cgcgagctgc agctccagat tcatcaaatt tgcatgtgct
     2041 gccgccaggc cttccaaaga ccccttaccg cggtctcggt gatgcgcgag tcgcccgagc
     2101 cgatggtggc gccgcccatc tcgatcaccg aggcgcgctc gggcgagccg gcgacgccgc
     2161 gctgtacgaa gccggtcgcg gcgccgtcct ccatggagat gtagcccgcc gggccgacca
     2221 ggttcgcctg cagcaggcgc cggcgcgtca tggcctcgtc gtcgtcggcg aaaccgaaga
     2281 gggtccagtg gagctcggtg cggtcgatgc ccttcggcac cacctggcgc gcggcgagcg
     2341 agttgcggat ctggtgcagc acgaagccgg ggaacagcga caggatctgg atcgagacgc
     2401 cgtcgccgag ctcgtcgacc tggtccagca cttcgggatc ctcgaggcgg aattcctgcg
     2461 agcccgcgga gcgcatgccg tccttctcgt aggcgtcgcc ccggtcctcg accatctcgg
     2521 tgtaggtgca gtggtgcgcg ccgtcgggcg agacgaacac gccgcctttc tgcgtcaggc
     2581 ggttgatgcg aaagcgcgtg aagaagaggt gcaggaggct cgcatggtag gtgtccttga
     2641 cgttgtccat gtagagcttc cagttgttcg gcagcacctg cgtatacgcg ccgagcacct
     2701 tgagcggctt gacgaggacg cgcttcatcc tcgggaggat ctcggggccg atataggctt
     2761 ccagggagga aacatccttg gagagcgtgc cgaacaccag gccgtgaacc gactcgaccc
     2821 tgagctgctt cggcgcctgc gactcgggcc tcgcgtcggc gggcatgccg cccttgccgg
     2881 cgatgccgcg acggaacgcg acgctggtca ggttgccggc gtggtcgtag gtccagttgt
     2941 ggtagatgca ggtgaagcgc tcggcggctc cgcgcggctg catgcacacc agcgcgccac
     3001 ggtgcgcgca gcggttctcc caggcgtgca gcgcgccctt cgggtcgcgc gtgacgacca
     3061 cgggcatgtc gccgacgaag gtggtgatga agcttttcgg ctcgggcagc tcggcctcga
     3121 ggcagagata gctccacgtg tcgccccgga agatcttcgc ctgctcctcg cggtagatcg
     3181 cctcgtcctg gtagacccag tacggaactc gcgtcgcgcc ttcggcgggc cagcttcgct
     3241 cgagcatcgg gtcattataa tgaggcgatg cgcgcggcct tcctgggaat ggtgttgctg
     3301 gcgacggcag cttcggcgca ggactggccg accaggaccg tgcgcatcgt cgtgccgttc
     3361 ccggcgggcg gcagcgcgga cctgctgccg cgcgtgatcg gcgagagcct gcagaagcag
     3421 tggggccagc cggtgattgt cgacaaccgc ccgggcgcgg cgggcaacat cggcgcggcc
     3481 gcggtgttcc aggcggagcc cgacggctac acattgctct ccgcgccgcc gccgccgctt
     3541 gttatcaacc ggctgctgta tccgaagctc gcgtacgact cgacgcagtt cgtgccgatg
     3601 acggtgatcg cggcgatccc gaacgtgctc ctggtgcatc ccaaggtcgg cgtcgcctcg
     3661 gtcgcggagc tgatcgctct cgccaaagcc aatcccggaa agctgaacta cgcctcgcag
     3721 ggcagcggcg ccacgtcgca tctcaccacc gagctcttca agtcgatggc gggcgggctg
     3781 agcatcaccc acgtgcccta caaaggcacc gcgcccgcgc tcgccgacct gctcgccggc
     3841 caggtcgacc tgatgttcga caacctcggc gtctcgctgc cgcacgtgaa gtcggggaag
     3901 ctgcgggcgc tcgcggtggc gagcacgcgg cgcttccgct cgctgcccga cgtgcccgcg
     3961 ctcgccgaaa cgctgcccgg cttcgaatcg gtggcgtggt tcggcatcgt cgggccgccg
     4021 aagacgccgg cggcgatcgc cgagaaggtg gcggcgggaa ccgccgaggc gctgcgcaac
     4081 cccgaggtgc tgcgccgcct gagcgatctg tcggcggacc cgctcggcat gacccccgcc
     4141 gagaccgccg cgttcatgaa gcaggagacc gagcgctggg gcgcggtgat tcgatccgcg
     4201 ggggtgaagc tcgagtaatt cggggacgga gcccgaacta attagggctc cgtccccgaa
     4261 ttacagccag ggcatcagcg aggcgtggat gacgctgggc aagcccatgc ccccgacgga
     4321 tttgatcgcg aggtccacgt ccttcagcgc gaagcggtgg gtcgtgacct tttccagggg
     4381 aaatcttttc gacgcgagct gggcgagcgc gagctcgcag gccttgtagc tgtggccgcg
     4441 cgcgctcttg atggtgatcg ccttggtcgt cacgcgctcg agcggaaagt tcgggaattc
     4501 cctgagctcg ccctgcacga cgatcgtccc ggccttgcgc ttcaaggctt ccacgcccag
     4561 gagtattgga atcgttcccg cgccggcggt gcagtcgagg accacgtcca cgcccttgcc
     4621 gccggtgatc tccctgatgc gggcgagcgg gtcttcggcg ttcacgtcga tggtgtggtc
     4681 cgcgccgaga gctttcgcgg tctccaatct cttcccgtcc ttggccgtgc cggtcactac
     4741 gatgagcgag gcgcccgcct gcttgcagat cacggtctgc gaaagcccct gttggcccgg
     4801 cccctggatg agcacggctg agtcgtagcc gacgccgccg tcgaagagcg accactcgac
     4861 gccgttggcc atcggcgtga cgagcccggc gagctccgcg cttacgccct gcggcacgcg
     4921 gtgcaccacc gcgttccacg gcaggtacag gtactgcgcg aagccgcccc agaggtgcgg
     4981 tgcgcgctct gccgaggtgt agccgtagcg gatgccctcc ggattggtcc gccagtcggt
     5041 gttctcgcag tggcggtact ggcccgcatg gcaccactcg cacgtgccgc acatcacgta
     5101 gtgctcgacg aagacgagat cgccttcctt gaatcccttg cgccgcacga actcgcgccc
     5161 ggccttggcg atcaccccga tgttctcgtg gcccatgatc accggcgcgc tcgagggcgg
     5221 ggtcttgtac agcttgacgt cggtgccgca gattcccgcg acctccatct tcaggagcgc
     5281 gctgtcctcc gggatctccg gcatggggta ttcgcggaac tcggtgcggc tggggccggt
     5341 gcggaccgcg gccaggactt tctcgctcat aatcggttca gtctaatgga acgagcagat
     5401 gcagcgagcc aggtaaaggc ttggacgcga agccatttcg gcgacgtcac ggtgctggtg
     5461 acggagctgg aaagcgcgct gcccggcttc ccgccgctgc acaccgtggt cgcgttctgg
     5521 accgcggaac gcaggcacta ccacttcaag gtgttcaagc cgctcgagca ggtggcggag
     5581 gaggacgtgc cgcccgcgtg gtaccgcgac gcgctcgccg tgacgccggg cacagactgc
     5641 ggctgctgct agcgctagac ttcgcggcag aaaagggggg atccatgaag tgtcgctggt
     5701 tcgtcgcgct ggctgtcgtc gcgggctttg gcattcacgc atgcgcgcag ctgccgtccg
     5761 atctcgccca gctgggaggc tggaccacgc tgatcgacgg agagaagggc ctggaaaact
     5821 ggacccgcgt cggcgacgcg aactggcgtc ccgaggacgg cgccatcgtc gcctaccgcg
     5881 gcaagggcgg gttcctggtg tccaagggct cctacaagga tttccagctc agggccgagt
     5941 tctgggccga cccgacgacg aacagcggcg tcttcatccg cgtctccaat cccaagcgga
     6001 tcggggcgga tagcgcctac gaagtgaaca tcttcgacca gccgccggac ccggcctacg
     6061 gcaccggcgc gatcgtgaac ttcgccaagg tgtcgccgat gcccaaggcg ggcggcaagt
     6121 ggaacaccct ggagatcacg gcccggggcc agcagctcac ggtgttgctg aacggcgtgc
     6181 agaccgtcga gctcaagcac gccaggttcg cccaggggcc ggtggcgctg cagtacggac
     6241 tcggcgccag gaacgccgag ggcgggccca tcaagtggcg ccgggtgcag gtcaagccga
     6301 tcgaggagaa ggagccggcc aaggccgccg ctgccaagcc ctcgtccggc ggcggcgctg
     6361 cctcgtcggg aggcggtagc gcgcccgcgc ggtaggttcc ttcagtttct cgcgagcagc
     6421 gtggacgcct ttgcggcgcc ggcgatggcg ttgtagcggt tcggttcctt gactagagtg
     6481 gcctcaaacg ccgcaagggc gtccctggcc atcccgcgct cgaggagcat cgcgccatag
     6541 agctcgcgcg ccggcgccag cggtcccggc gtgaccacgg atttttcggt ccgatcctcg
     6601 gcgtcggcgg cccggccgag cgtgctcagc gcttcgtcgt gcttgccctc ggcgtgcagc
     6661 agccaggccg tggcgaccag ccattggatc tcgacctgct ccgcccagta ggcgtccttc
     6721 gccgagcgca gcttgtcgcg cagctcggcg agcttcgcga tgtcggccct ggcggtttcc
     6781 gggttgccgg agcgcgccgc gccgagcgcc cgcgcgaaat gcgtgatcgc atcgacatag
     6841 gcgaagcggg tggggcgcac ctcgagctcc gccgcggctt tccagtcatt gcgttcgacc
     6901 gcgtagcgcg ccgtgctcgc ggcgatcgca tacggtccgg tacggatatt cgggttgaag
     6961 cccttggtgt cgatcatctg atcgatgact gcgcgcgcct cccggtgctg cccaagctgg
     7021 agatgcgcat agaccaggta atccatcgcg tgcaacacgt cgtgcagtcc ccgggcggca
     7081 ttggcgccct gcgccgcctc gcgcgccacg cgggccgatt cccgattcga ttcgatcgac
     7141 tccttccacg cgccgacccg cgtgaagatg tgcgacggca tgtgcagcgc gtgcgcggca
     7201 tcgggcgcga tccggctgta gcgccgcgcg gcgtcgagcc cttggctcgc gatcggcgga
     7261 tagtcgtagc tgtggatgag gtagtgcgcg acgcccggat gctgcggctg ctccttgaag
     7321 atcggttcca agatcgccgc cgccttcagc tgattggtgt acttgcggtc cgcgggatcg
     7381 aagtttgcgg acaggacgag cgcgtacagg gtcgccgcct ccttgtcctg cggatagcgt
     7441 tgcatcacct gctccatcgc ggcttcgagc gccttggcgc ggctgcgatg gtcgcgcttg
     7501 tcggcgtcgc ggaagaacac ggccagcgca tccacgtagt cgcgctcgcg ctgcgacttc
     7561 aggccggtgc gccgcgccgt ctcgaggatg cccggcccct ggctcagcgc ggccggcgaa
     7621 atgatccccg gccaggcgaa cgggttgtcg agcgatgcca gcgcgcgcgc ccagtgcgcc
     7681 atgccgcagc ccgggtcggc ctggagcacg ccctcgagcg gctcgcggat ctgcgtccag
     7741 gcgaacgagt ggtagtacgc cagggcgcga tcgaaatcgc gctgcgccgc cgcgttgcat
     7801 tcgacggcaa agtggacttt ccccaggcgt acgtgcgcgc cgtgcccttc gtgaggatcc
     7861 tgggttgcgc aggcagcgat tccgagtgac agcaaagccg ggaggatttt catggcggca
     7921 gagtgtgggc cgccgctccc tgcgggtcca atacattgtt tctctggtcg ttcatgccgg
     7981 agcggcataa actcgaagcg tggacctgcg ccagctgcga accttcgtca ccgtcgccga
     8041 gcagggcacg gtgtccaagg cagcgctgcg cctgcgcatc gcgcagcccg cgctgtcccg
     8101 gcagatcagc catctcgaag acgagctcgg cgtcaagctg ttcgaccggg tgcgccgccg
     8161 gctggtgctg acgggcgaag gcgagcagct gctcggcgac tgccgcacgg tgctgggcgc
     8221 ggtcggctcg ctgagcgagc gcgcgcagct gctgcggcgc ggcgacagcg gcgtcctccg
     8281 ggtggcggcg acgccgcaga tgatcgacgg ggtcttttcg aacttcctgc accgctatgc
     8341 cgagcggcgg cccaaggtgc agatcaagct gaccgaggcg gccgggtccg gcctgctggg
     8401 gatgctggag cgcggcgagc tgcacgtctg catcaccacc gcgcaggtgc tgcagcccga
     8461 caagaacgcc ttcgacacgt tcgcgctgcc gcccatcgag ttcctcgcgg cctcccacga
     8521 ttcgctcaag ggcggcgctt ccggcaacgt cgatatcgcg cgccttgcag cctatccgct
     8581 gctgctcctg gagagcactt tcttcgtccg tacggcgttc gacgcggcgt gccgcctcgc
     8641 cggcttcaag cccaatgtct tcatcgagag ccggacgccg cacaccttgc tgtcgctggc
     8701 cgaggccggg cacggcgtgg cgatcgtgcc ctcggtactg cccacccatc gctaccggct
     8761 acgcatcgcg cgcatcacgc accggcgcaa gccgttgcgg cagccgctcg acgtcgtctg
     8821 ggaccggcgg cgcttgctgc cgccgtatgc gaaggatttt tgcgaggcgc tggccgcgca
     8881 catgcggcag gtgttcccga tctcgcgacc cggcgtgcgg gcgcgggcgg cgcggtagat
     8941 cccttccggg cgggttttgt ttgaaataca ctgtcggcac aacagaggag acaccatgaa
     9001 aagaacgctg ctcgctggtc ttgctctcgg cttcacgatc atcggctgct cgcagatgcc
     9061 ttcctgggtg ccggggagcg gctggaccac gctgatcgac ggcgacaagg ggatggagaa
     9121 cttcgaccgc gtcggcgacg ccaactggcg cgccgagggc ggcgcgatcg tcgccgacaa
     9181 gggcaagggc gggttcctgg tgtcgaagaa ctcgtacaag gacttcgacc tgcgcgccga
     9241 gttctgggcc gacaacaaca cgaacagcgg catcttcatg cgctgcgccg accgcaagac
     9301 gatcaccgac aagagctgct acgaggccaa catcttcgac cagcggcccg accagacctt
     9361 tgccaccggc ggcatcgtgc acttcgccaa ggtgctcgcg ccggcgaagg ccggcggcaa
     9421 gtggaacacc tacgagatct ccgcgcgcgg atccaaggtg accgtgaccc tgaacgggac
     9481 gaagaccgcc gagatggacc acagccagct cgcctccggc ccggtcgcgc tgcagttcgg
     9541 cagcctgccg ccgaagggcg acccgggcgg ctcgatcaag tggcgcaaag tgcagatcag
     9601 accgctgtaa gagagtaagg gagaccgcca tgaaacgcct gctcgtcatt gcaacgggat
     9661 tgctcgtcgc cgcctgctcg tcgctgcctg cctgggttcc agggagcggc tggaccaccc
     9721 tcatcgacgg cgagaagggg ctggagaact tcacccgcga gggcgacgcc aactggcgcg
     9781 ccgagggcgg cgccatcgtc gccgacaagg gcaagggcgg ccacctggtg acgaagagct
     9841 cgtacaagga tttccagctc cgcgccgagt tctgggccga ccaccacacg aacagcggca
     9901 tcttcatgcg catccaggac ccgaagaaga tcggcgcgaa cagcgcctac gaggtgaaca
     9961 tctacgatca gcgcgccggt cccgagttcg gcacggccgg gatcgtcgac ttcgccaagg
    10021 tcccgccgca caagtacaag gccggcggca agtggaatac gttcgagatc accgccaagg
    10081 gctcgcagct caccgtcgtg ttcaacggcg acaagaccgt cgacatccag aacgccaagt
    10141 tcgcgcaagg gccgttctcg ctgcagttcg gcaacctgcc gccgaagaac gcgcccggcg
    10201 gcgccatccg ctggcgcaag gtgcaggtca gggagctcta agaagtcgct gagcggtgtt
    10261 catcgggcac ttcgcggtcg ccttcgccgc gaagcgcgcg gcgcccgagg tgtcgctcgg
    10321 cacgctgttc ctcgcggcgc agctcgccga tctcgtctgg ccgacgctcg tgctgctggg
    10381 gctggagcgt ttcgagatcc gccccggcat caccgcggtc acgccgctcg acttcgtcca
    10441 ctacccgtac tcgcacagcc tggtagcgct ggcaggatgg gctttcgccc tgggtttcct
    10501 gtattacgca ttcaggagag gaggacgcgg cgcggcgatc ctcttcgcag tcgtgctcag
    10561 tcactggctg ctcgacttcg caacccaccg ccccgacatg ccgctgacca tcggcggcga
    10621 ggagcgcttc ggcctcgggc tgtggcattc gcgcatggca acgctggcgg tcgaggggct
    10681 gctgttcggc gcctgcgtgt ggctctacgc gcgcaccact cgcgcgctgg accgcaccgg
    10741 ccgctggtcg ttcgtcgcct tcgtggcatt tctcatggtc gtgtacgtgg ccaatgtctt
    10801 cggcccgccg ccgccgagcg ttgcggcagt ggcgtggagc gcgcaggcga tctggctact
    10861 ggtggcctgg ggctactgga tcgaccgtca tcgcgcggcg tcgaggaact gaatgaggcg
    10921 cggggcggcc gcggcgtcgg tctgcgcctc gggcagcgcg tcccagtact gcgatttcgg
    10981 caggcattcc tcgaggtagc gcgcggccga ggtcgcgtgc gactcgtcgc ggccggggac
    11041 gacgagggcg gggatgtcga gcttcagcag gtcctccggc acggcgcccg gcgaggtgtc
    11101 gcggtcgaac agcgcgtcgc g
//
LOCUS       DATBSZ010000022         1970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_685678, whole genome shotgun sequence.
ACCESSION   DATBSZ010000022 DATBSZ010000000
VERSION     DATBSZ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685678"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VIG70_01410"
     CDS             <1..421
                     /locus_tag="VIG70_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEY8249234.1"
                     /translation="VYGRSDATLNPGGVRIGTAEIYGVVERIDEVEESVAIGQLWPPD
                     SPTDTRIVLFVKLRSGYGLDDALEDRIRHEIRRHASPRHVPARIIQVADIPRTKNGKV
                     VELAVKAVVHDMPVTNADSLANPEALELFRNLEELAT"
     gene            complement(425..856)
                     /locus_tag="VIG70_01415"
     CDS             complement(425..856)
                     /locus_tag="VIG70_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8249235.1"
                     /translation="MERPVRSIIEDQKPVTASAGITVASAARLMKEHRIGAILVIQDG
                     RLAGIFTERDALFRVIAEGRDPAKTRVGEVMTANPRTIAPDRPFGHALHMMYEGGFRH
                     VPVVDDGRPLGVVSSRDALGPDLQQFIAELDKRTHIGEILG"
     gene            complement(882..1259)
                     /locus_tag="VIG70_01420"
     CDS             complement(882..1259)
                     /locus_tag="VIG70_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEY8249236.1"
                     /translation="MKERIVRGIVLFAHGSRDPAWSRPFESIASQLSKKFLVRMAYLE
                     QMRPSLGEAVSALAAAGAKSIRVVPLFLGPGGHVKDDLPRLVAAARTGHPHVEITLEK
                     PIGEQPTVITAIAEAISSAIPKE"
     gene            complement(1256..1921)
                     /locus_tag="VIG70_01425"
     CDS             complement(1256..1921)
                     /locus_tag="VIG70_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8249237.1"
                     /translation="MSSGRVFRFAPTPERRPNHLFVFLHGCGATAQSMIPIAFKFQAR
                     FPSAALVVPSGFDPDARGGAAQDWYPTRGLNCDNHRERVAAVLPDVEDLVRREQINFT
                     VPASRTVLIGFSQGGTVALEVVKSPGIAGAVVAFSSRFAQTPGPGSRISSRIHLVHGE
                     FDSVVSRVYAERAARVLAGLHVPVTLDIVEDLGHALSHRAISLGSLRLLQGIYEGRRA
                     TLH"
BASE COUNT          336 a          655 c          676 g          303 t
ORIGIN      
        1 ggtgtacggg cgctcggacg cgaccctcaa tcccggcggc gtgcgcatcg gcaccgccga
       61 gatctacggc gtggtggagc gcatcgacga ggtggaggag tcggtggcga tcggccagct
      121 ctggccgccg gacagcccta ccgatacgcg catcgtgctc ttcgtgaagc tgcgctcggg
      181 ctacggcctc gacgacgcgc tcgaggaccg catccgccac gagatccggc gccacgccag
      241 cccgcgccac gtcccggcgc gcatcatcca ggtcgccgac atcccgcgca ccaagaacgg
      301 caaggtcgtc gagctcgccg tgaaggcggt ggtgcacgac atgccggtca ccaacgccga
      361 ctcgctggcg aacccggagg cgctcgagct tttcaggaac ctcgaagagc tagcaacctg
      421 attcctaccc caggatctcg ccgatgtggg tgcgcttgtc gagctcggcg atgaactgct
      481 gaaggtccgg gccgagcgcg tcgcgcgacg agaccacgcc gagcggccgg ccgtcgtcga
      541 ccaccggcac gtggcggaag ccgccctcgt acatcatgtg cagcgcgtgg ccgaacggcc
      601 ggtcgggcgc gatggtgcgc gggttggccg tcatcacttc tccaacccgt gtcttggcgg
      661 gatcgcggcc ctcggcaatc acgcggaaaa gcgcatcgcg ctcggtgaag atgcccgcga
      721 gcctgccgtc ctggatgacg aggatcgcgc cgatgcgatg ctccttcatg aggcgcgccg
      781 ctgaagccac ggtaatgccg gcgctggcgg tgacgggttt ctgatcctcg atgatgctgc
      841 ggactgggcg ttccatggcg ggcgtcctcc cgcgtatcac tctactcctt cgggatggcc
      901 gaagaaatcg cctccgcgat ggcggtaatg accgtgggct gctcgccgat cggcttttcc
      961 agcgtaattt caacgtgcgg atggccggtc cgggcagcgg ccacaaggcg gggcaggtcg
     1021 tccttgacgt gtccgccggg gcccagaaaa agcggcacca cccggatcga ctttgcgcca
     1081 gcggccgcca gcgcgctgac ggcttcgccg agcgagggac gcatctgctc gagatacgcc
     1141 atacgcacga ggaatttctt ggacagttgc gaggcgatgc tttcgaacgg ccgcgaccac
     1201 gcggggtcgc gtgacccgtg agcaaaaagg acaatgcccc ggacgatcct ttccttcaat
     1261 gcagggtggc gcggcgccct tcgtagatgc cctgcagaag ccgcagcgag cccaggctga
     1321 tggcgcgatg cgacaacgcg tgtcccaggt cctcgacgat gtcgagcgtg accgggacgt
     1381 gaagcccggc gagcacgcgc gccgcgcgct cggcgtacac gcgcgacacc accgagtcga
     1441 actcgccgtg caccagatgg atgcgcgagg agatacggct gccggggccg ggcgtttgcg
     1501 cgaagcgcga cgagaacgca accactgcgc cggcgatgcc cggcgacttc accacttcga
     1561 gcgcgacggt gccgccctgc gagaagccga tcagcacggt gcggctcgcc ggcaccgtga
     1621 aattgatctg ctcgcggcgt accaggtcct cgacgtcggg gagcaccgcg gcgacgcgct
     1681 cgcgatggtt gtcgcagttc aggccgcgcg tcgggtacca atcttgcgcc gctccgccgc
     1741 gcgcgtcggg atcgaagccg ctcggcacca ccagcgccgc gctcggaaag cgcgcctgga
     1801 acttgaacgc gatcgggatc atcgattgcg cggtggcgcc gcatccgtgc aggaagacga
     1861 acaggtggtt tggccggcgc tctggcgtgg gggcgaagcg gaaaacccgg cccgaagaca
     1921 ttgcccccga ttgttaaccc gtgacgacag ttctgtaaac tgatttgcat
//
LOCUS       DATBSZ010000023         4704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1642030, whole genome shotgun sequence.
ACCESSION   DATBSZ010000023 DATBSZ010000000
VERSION     DATBSZ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642030"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1642
                     /gene="ftsH"
                     /locus_tag="VIG70_01430"
     CDS             <1..1642
                     /gene="ftsH"
                     /locus_tag="VIG70_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY8249238.1"
                     /translation="YDGKVTITAKPLGDNVPWFVSLLVSWLPFIALIGVWVFLSRQMQ
                     GGAGKAMGFGKSRAKMLTEAHGRVTFEDVAGVDEAKQDLQEIVEFLRDPGRFQKLGGR
                     IPRGVLMVGSPGTGKTLLARAIAGEAKVPFFSISGSDFVEMFVGVGAARVRDMFEQAK
                     KHAPCIVFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLVEMDGFEATMGVIVIAATNR
                     PDVLDPALLRPGRFDRQVVVPLPDIRGREEILKVHMRKVPLAPDVKPDIIARGTPGFS
                     GADLANLVNEAALFAARGNKRLVDMDDFERAKDKIIMGAERRSMVMPEEERRNTAYHE
                     SGHTIVARLLPKTDPVHKVTVIPRGRALGVTMQLPTEDRYSQDRDRLLNMIAVLFGGR
                     IAEELFMNQMTTGASNDFERATEIARRMVTQWGMSDELGPMVYGENEGEVFLGRSITT
                     HKNVSEATLQKVDAEIRRIIDQQYRLARGLLEGNRDKVEAMAKALLEYETLDADQIGD
                     IMSGKPPRPPKPSGSQPAAKPPAADGGAEAATAPTPAA"
     gene            1692..2450
                     /gene="folP"
                     /locus_tag="VIG70_01435"
     CDS             1692..2450
                     /gene="folP"
                     /locus_tag="VIG70_01435"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY8249239.1"
                     /translation="MGVVNITPDSFSDGGRFLGANEAITHARRLADEGADIIDLGGES
                     TRPGAVPVSEDEELRRVVPVLEKLPDLCVSVDTRRTAVMKAALAAGASMINDVDALGA
                     PGALEAVAAGSCAVCLMHKKGEPATMQRDPHYDDVVGEVKGFLNQRIEAAKGAGIAAG
                     RIVVDPGFGFGKTADHNLALLRKLSELSEFPILVGLSRKSTLGKITGRAVDERLAGSL
                     TMALLALQRGATILRVHDVRETRDVVAVWQAYNA"
     gene            2447..3808
                     /gene="glmM"
                     /locus_tag="VIG70_01440"
     CDS             2447..3808
                     /gene="glmM"
                     /locus_tag="VIG70_01440"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY8249240.1"
                     /translation="MRKYFGTDGVRGTVGQNPMTPEFILRFGYAAGVVLAERETRGQD
                     KHPAVCIGKDTRISGYMIESALEAGFSAAGVDVHMCGPIPTPAVAYLARALRLSAGIV
                     ISASHNPYPDNGIKIFSGDGFKLPDDIEAEIEAQMEQPMGCNEASRLGKVWRVDDAGG
                     RYIEFCKSTFPSQLDLKGMRIVLDCAHGAAYNVAPHVFHELGADVSAIGVKPDGMNIN
                     ERTGATSPAALQRQVKDERADLGIALDGDADRVLMVDSSGQRYDGDQLLYVIANHRAK
                     DRRAGGKQKGVAGTLMTNFAFEKAMARLGIAFGRAKVGDRYVLEMMREKGWELGGENS
                     GHVICLDKHTTGDGIVSALQVLHVMRESGRSLAELTADLVMFPQVLLNVAVPRGFQWD
                     KHEGIRKAHADAEQSLNGKGRVLLRPSGTEPVLRVMVEGEPRDAIESAAKSIADAVKQ
                     AAR"
     gene            3805..>4704
                     /locus_tag="VIG70_01445"
     CDS             3805..>4704
                     /locus_tag="VIG70_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8249241.1"
                     /translation="MSKPAHVVIALGLAQTLAWGSTYYLPAILANAMAAELGLATAWV
                     FTAFSAGLLVSAFLGPAAGRLIDLYGGRRVLPASNLVFAAGLAMLGTSAGVVSLFASW
                     LVIGIGMSAGLYEAAFSTLAGIYGREARRSITGITLIAGFASTVCWPLSAYMDVTLGW
                     RATCYVWALAHLAIGLPINLLLPARRAADKAPQPSASEAVERGRLVLMAGLSFVFAAT
                     WFCSTAMAAHLPRVLQESGASLATAVAAAALVGPAQVGARVLEFWLMRHVHPIVSARI
                     ATLAHPIGAAGLIAAGSPAATAFA"
BASE COUNT          765 a         1564 c         1645 g          730 t
ORIGIN      
        1 ctacgacggc aaggtgacca tcaccgcgaa gccgctgggc gacaatgttc cgtggtttgt
       61 ttcgctgctc gtctcgtggc tgcccttcat cgcgctgatc ggcgtgtggg tgttcctgtc
      121 gcgacagatg cagggcggcg ccggcaaggc gatgggcttt ggcaagtcgc gcgccaagat
      181 gctgacggag gcccatggcc gcgtcacctt cgaggacgtc gcgggtgtcg acgaggccaa
      241 gcaggacctg caggagatcg tcgagttcct gcgcgacccg ggccggttcc agaagctcgg
      301 tggccgcatc ccgcgcggcg tgctgatggt cggctcgccg ggcaccggca agacgctgct
      361 cgcgcgcgcc atcgcgggcg aagcgaaggt gccgttcttc tcgatctccg gctccgattt
      421 cgtcgagatg ttcgtcggcg tcggtgcggc gcgcgtgcgc gacatgttcg agcaggcgaa
      481 gaagcacgcg ccgtgcatcg tgttcatcga cgagatcgac gcggtcggcc gccagcgcgg
      541 cgcgggcctc ggcggcggca acgacgagcg cgagcagacg ctcaaccagc tcctcgtgga
      601 gatggacggc ttcgaggcga ccatgggcgt gatcgtgatc gcggcgacca accgtcccga
      661 cgtgctcgac ccggcgctcc tgcgcccggg ccgcttcgac cggcaagtgg tggtaccgct
      721 gccggacatt cgtgggcgcg aggaaattct gaaagtgcac atgcgcaagg tcccgctcgc
      781 gccggacgtc aagcccgaca tcatcgcgcg cggcaccccc ggcttctcgg gggccgacct
      841 cgcgaacctg gtgaacgaag cggcgctgtt cgctgcgcgc ggcaacaagc gcctggtgga
      901 catggacgac ttcgagcgcg cgaaggacaa gatcatcatg ggcgccgagc ggcgctcgat
      961 ggtgatgccc gaggaagagc gccgcaacac cgcgtaccac gaatccggcc acaccatcgt
     1021 cgcgcgcctg ctgccgaaga ccgatccagt gcacaaggtg acggtgattc cgcgcggccg
     1081 cgcgctgggc gtgacgatgc agctgccgac ggaagaccgc tacagccagg accgcgaccg
     1141 gctgctgaac atgatcgcgg tgctgttcgg gggccgcatc gccgaagagc tgttcatgaa
     1201 ccagatgacc accggcgcgt cgaacgactt cgagcgcgcc accgagatcg cgcgccgcat
     1261 ggtgacgcag tggggcatgt ccgacgagct cgggccgatg gtgtacggcg agaacgaggg
     1321 cgaggtgttc ctcggccgct cgatcaccac gcacaagaac gtctccgagg cgacgctgca
     1381 gaaggtggac gccgagatcc ggcgcatcat cgaccagcag taccgcctgg cgcgcgggct
     1441 gctcgagggg aaccgggaca aggtcgaggc gatggcgaaa gcgctgctcg agtacgagac
     1501 gctcgacgcc gaccagatcg gcgacatcat gtcgggcaag ccgccgcggc cgccgaagcc
     1561 gagcggctcg cagcctgcgg ccaagccgcc ggccgccgac ggcggcgccg aggccgcgac
     1621 cgcgccgacg cctgcagcct aagatccttc gctgcggcag attcagcctg ccgctcgaaa
     1681 agcccctggt catgggcgtg gtcaacatca cgcccgattc cttctccgac ggcggccgct
     1741 tcctgggtgc taacgaagcg ataactcatg ctcgccggct cgccgatgaa ggtgcagaca
     1801 tcatcgacct cggcggcgaa tcgacgcggc cgggcgccgt gccggtttcc gaagacgagg
     1861 agctgcggcg cgtcgttccg gtgctcgaaa agctccccga cctgtgcgtt tccgtggaca
     1921 cgcgccgcac cgcggtgatg aaagctgcgc tggcggcggg cgcctccatg atcaacgacg
     1981 tcgacgccct gggcgcgccc ggcgcgctcg aagcggtggc ggcgggctcg tgcgcggtct
     2041 gcctcatgca caagaaaggc gagcccgcga ccatgcagcg cgacccgcac tacgacgacg
     2101 tcgtgggcga ggtgaaaggc tttctcaatc aacgaattga agcagcaaag ggcgccggga
     2161 tcgcggccgg gcgcatcgtc gtcgacccgg gcttcggctt cggcaagacc gcggatcaca
     2221 acctggcctt gctgcggaag ctttcagagc tttccgagtt tcccatcctc gtcggtctct
     2281 cgcgcaagtc gacgctgggc aagatcaccg ggcgcgccgt cgacgagcgg ctggccggca
     2341 gcctgacgat ggctttgctc gcgttgcagc gcggcgcgac aatactgcgg gtgcatgacg
     2401 tgagagagac gcgcgacgtg gtggcagtct ggcaggccta taacgcatga gaaagtactt
     2461 cggcaccgac ggtgttcgcg gcacggtggg gcagaacccc atgacgccgg aattcatcct
     2521 gcgcttcggc tatgccgcgg gcgtggttct cgccgagagg gagacgcgcg gccaggacaa
     2581 gcatccggcg gtgtgcatcg gcaaggacac gcgcatctcc ggctacatga tcgagtcggc
     2641 cctggaggcc gggttctctg ccgcgggcgt cgatgtccac atgtgcggcc cgatccctac
     2701 gccggcggtc gcctatctcg cgcgtgccct gcggctttcg gccgggatcg tcatcagcgc
     2761 gtcgcacaac ccgtaccccg acaacggcat caagatcttc tccggcgacg gcttcaagct
     2821 gcccgacgac atcgaggccg agatcgaggc gcagatggag cagccgatgg gctgcaacga
     2881 ggccagcagg ctgggcaagg tctggcgagt ggacgacgcc ggggggcgct acatcgagtt
     2941 ctgcaagtcc acttttccgt cgcagctcga cctgaagggc atgcgcatcg tgctcgactg
     3001 cgcccacggc gccgcgtaca acgtcgcgcc gcacgtgttc cacgagctcg gcgccgacgt
     3061 gagcgccatc ggggtcaagc ccgacggcat gaacatcaac gagcgcacgg gcgcgacgtc
     3121 gccggcggcc ctccagcgcc aggtgaagga tgagcgggcc gacctgggga ttgccctcga
     3181 cggcgacgcc gatcgcgtgc tgatggtcga cagctcggga cagcgctacg acggcgacca
     3241 gctgctctac gtgatcgcca accaccgcgc gaaggatcgg cgagccggcg gcaagcagaa
     3301 gggcgtggcc ggcacgctga tgaccaactt cgccttcgag aaggcgatgg cgcggctcgg
     3361 catcgcattc ggccgcgcga aggtcggcga ccgctacgtg ctcgagatga tgcgcgaaaa
     3421 gggctgggag ctcggcggcg agaactcggg ccatgtcatc tgcctcgaca agcacaccac
     3481 cggcgacggc atcgtgtccg cgctgcaggt cctgcatgtc atgcgcgagt cgggccgctc
     3541 gctggcggag ctcaccgcgg acctggtcat gttcccgcag gtgcttctga acgtggcggt
     3601 gccgcgcggg ttccagtggg ataaacatga aggaataaga aaagcgcatg cagacgccga
     3661 gcaatcgctc aacggcaagg gccgcgttct gctgcggccc tcgggcaccg agccggtgct
     3721 gcgcgtgatg gtggagggcg agccgcgcga tgcgatcgaa tccgcggcga aaagcatcgc
     3781 ggatgccgtg aagcaagcgg cgcgctgagc aagcccgccc atgtagtcat cgcgctcggg
     3841 ctggcgcaaa cgctggcctg gggatcgacc tactaccttc cggcgatcct cgcgaacgcg
     3901 atggcggccg agctcggcct ggccacggca tgggtgttca cggcgttttc cgccgggctg
     3961 ctcgtctccg cgttcctcgg gccggcggcg ggccggttga tcgacctcta cggcgggcgc
     4021 cgagtgctgc ctgcctccaa cctggtgttc gccgcgggac tcgcgatgct gggcacgtcc
     4081 gccggggtcg tatccctctt cgcgagctgg ctcgtcatcg gtatcggcat gagcgcaggt
     4141 ttgtacgagg cggcgttctc taccctcgcc ggaatctacg gacgcgaagc gcgccgctcg
     4201 atcaccggca tcaccctgat cgcgggattc gccagcaccg tctgctggcc gctttcggcg
     4261 tacatggacg tgacgctggg gtggcgcgcg acctgctacg tctgggcgct cgcgcatctc
     4321 gcgatcggcc tgccgatcaa cctcctgttg ccggcgcggc gcgccgccga caaggcgccg
     4381 cagccgagcg cctcggaagc ggtcgagcgg ggtcgactcg tactgatggc aggcctttcg
     4441 ttcgtcttcg cggcgacctg gttctgcagc actgccatgg cggcgcatct tccccgagtc
     4501 ctgcaggaat ccggcgcaag cctggcgacg gcggtggccg ctgcggcgct ggtcggcccg
     4561 gctcaggtcg gggcgcgcgt tctcgagttc tggctgatgc gccacgtcca cccgatcgtt
     4621 tccgcgcgca tcgccacgct ggcccatcca atcggcgctg ccggattgat cgccgctggc
     4681 tccccagccg ccactgcgtt cgcg
//
LOCUS       DATBSZ010000024         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_344479, whole genome shotgun sequence.
ACCESSION   DATBSZ010000024 DATBSZ010000000
VERSION     DATBSZ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344479"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VIG70_01450"
     CDS             complement(<1..939)
                     /locus_tag="VIG70_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HEY8249242.1"
                     /translation="MCGIGGFFALRPVAPQVVSSMQAALRPRGPDAQHAQLWGQNGLL
                     HARLSIIDPRPEADQPMANARGDVWIVYNGEVYDWQDAAAALQAAAPFRTRSDTEFIL
                     RGYEAWGIGALLERLRGMFAFAILDLARGKAYLARDRMGEKPLVYASVGEELAFGSTV
                     RSVLPFLPREKRELSPEAIDGYLAHRYIPAPRTVYRHVQRLENGHYLEFDLRSRRLEK
                     RRYWQAAPEPGSWLEALDESVRMRTIADRPLGVFLSSGVDSTVVASRLATQRLTSYRT
                     YTAAFPGSDMDESALAAQAAATLGFPNTPVAVPQSLG"
     gene            complement(942..>2245)
                     /locus_tag="VIG70_01455"
     CDS             complement(942..>2245)
                     /locus_tag="VIG70_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8249243.1"
                     /translation="PSPKTVYRGVRKLEPGHALVVAGGKLSLRRYWLPDYSEKFDLGE
                     TEALAGLEAELRRSVESMLVSDVPLGSFLSGGVDSSVVSALMADIARRPIDTFTLGFH
                     GESAVSEHSHAALVSRHIGAVNHVLMLDPSDMLAAIEQWTEAFDEPFADPAALPTMLL
                     AKLTRQHVTVVLTGEGADEVFSGYGNYRKRVREERITGVLGARLSPLRYLVRALPARA
                     RKDRLLKAIGEPLSRRYVTIPMVFDPALHAELLSERFLAARETSMADYAERHFDECNS
                     PHYIDKLMYVDARLWLPDDLLTKVDRATMAYSLEARVPYLDHRLFEFCARLDPGLKQR
                     GRTGKYLLKRLAEKLLPPEIVHRPKQGFFPPLTEWFAGRLKEEARAALSALDKRGLFR
                     AGALQTLSQEHYSGGRNHSGRLWALFVLEKWFRRYAPGFSL"
BASE COUNT          368 a          745 c          805 g          327 t
ORIGIN      
        1 accgagcgat tgcggaacgg cgaccggggt gttcggaaat cccagcgtcg cggcggcctg
       61 cgcggcgagc gccgattcgt ccatgtcgct gccggggaag gcggcggtat aggtgcggta
      121 ggaagtcagg cgctgcgtgg caaggcgact cgcgacgacg gtcgagtcga caccgctcga
      181 gagaaatacg cccagcggac gatcggcgat ggtgcgcatg cgcaccgatt cgtcgagcgc
      241 ctccagccag gatccaggct cgggcgccgc ttgccaatag cgccgttttt ccaggcgccg
      301 ggaccggaga tcgaactcga ggtagtggcc gttctccagg cgctggacgt gacggtatac
      361 ggtgcgcggc gccggaatgt agcggtgcgc gagataccca tcgatcgctt cgggcgagag
      421 ctcgcgcttc tcccgcggca ggaacggcag caccgagcgc acggtggagc cgaaggcgag
      481 ctcctcgccg accgaggcgt acaccagcgg cttctcgccc atgcggtcgc gggcgagata
      541 ggccttgccc cgggcgaggt cgaggatggc gaacgcgaac atgccgcgca gccgctcgag
      601 caacgccccg atcccccagg cctcgtagcc gcgcaggatg aactcggtgt ccgagcgggt
      661 gcgaaacggt gcggctgcct gcagcgcggc ggccgcgtcc tgccagtcgt agacctcccc
      721 gttatagacg atccagacgt cgccgcgcgc gttggccatc ggctggtccg cctcgggccg
      781 gggatcgatg atcgacaggc gcgcgtgcag caggccgttc tggccccaga gctgcgcgtg
      841 ctgcgcgtcg gggccgcgcg gccgcagcgc cgcttgcatc gagctcacga cctgcggcgc
      901 caccggccgc agcgcgaaaa agccgccgat tccgcacatg gctagagcga gaaccccgga
      961 gcgtagcgcc ggaaccactt ctccaggacg aacagcgccc acagccgccc ggaatggttg
     1021 cggcctcccg agtaatgttc ctgggacagg gtttgcaaag ccccggcgcg gaacagcccc
     1081 cgcttgtcca gcgccgaaag cgcagcgcgc gcttcctcct tgagccggcc ggcgaaccat
     1141 tcggtgagtg gcgggaagaa gccctgcttc ggtcggtgca cgatctcggg cggcaggagc
     1201 ttctcggcga gccgcttcag gagatatttg ccggtgcggc cgcgctgctt gagcccgggg
     1261 tcgaggcgcg cgcagaactc gaacaggcga tggtcgaggt aaggcacgcg cgcctcgagc
     1321 gaatacgcca tggtcgcgcg atcgaccttg gtgagcaggt cgtcaggcag ccagagccgc
     1381 gcgtcgacgt acatgagctt gtcgatgtaa tgcggcgagt tgcactcgtc gaaatggcgc
     1441 tcggcgtagt cggccatgga ggtttcgcgc gctgcgagga agcgctccga gagcagctcc
     1501 gcgtgcagcg cgggatcgaa gaccatcgga atggtcacgt agcggcgcga caggggctcg
     1561 ccgatcgcct tcagcagccg gtccttgcgc gcgcgggcgg gcagcgcgcg caccaggtag
     1621 cgcagcggcg agagccgcgc gcccaggacc ccggtgatgc gctcttcgcg cacgcgcttg
     1681 cggtagttgc cgtagccgga aaacacctcg tcggcgcctt ccccggtgag gaccacggtc
     1741 acgtgctgcc gcgtcagctt ggcgaggagc atggtcggca gcgccgcggg atcggcgaac
     1801 ggctcgtcaa acgcctcggt ccattgctcg atcgccgcca gcatgtcgga ggggtccagc
     1861 atcagcacgt ggttcacggc gccgatgtgg cgcgagacga gcgccgcatg cgagtgctcg
     1921 ctcaccgcgc tctcgccgtg gaacccgagc gtgaacgtgt cgatcggccg tcttgcgatg
     1981 tcggccatca gcgcgctgac cacgctcgaa tcgaccccgc ccgaaaggaa gcttcccagc
     2041 ggcacgtcgg agacgagcat gctttccacc gagcggcgca gctcggcttc gaggccggcc
     2101 agggcctcgg tttcgccgag gtcgaatttc tccgaatagt ccggcagcca gtaccggcgc
     2161 agggaaagct tgccgcctgc gacgacgagc gcgtgcccgg gctcgagctt gcgcaccccg
     2221 cggtacacgg tcttcggcga cggga
//
LOCUS       DATBSZ010000025         6595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_753936, whole genome shotgun sequence.
ACCESSION   DATBSZ010000025 DATBSZ010000000
VERSION     DATBSZ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753936"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VIG70_01460"
     CDS             complement(<1..615)
                     /locus_tag="VIG70_01460"
                     /inference="COORDINATES: protein motif:HMM:NF018275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEY8249244.1"
                     /translation="MRALFAKPLSVLNVGLASFADAIVQRGGAATRIEWAPPANGDAA
                     VGAALARLVNDEKMEAANAQAAERYLAAQPRLEGIGIAREVLPGMGSRMILHAGPPIV
                     WPRMCGPMQGAIVGAILYEGWADDAAKARAMAESGDIAFEPCHHHAAVGPMAGIISPS
                     MPVWMVRNPTHGNRTFSNLNEGLGKVLRFGANGPDVIARLKWMEK"
     gene            complement(612..2117)
                     /gene="fdrA"
                     /locus_tag="VIG70_01465"
     CDS             complement(612..2117)
                     /gene="fdrA"
                     /locus_tag="VIG70_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HEY8249245.1"
                     /translation="MYIHRVLPNLYRDSVSLMQLSATLGKLPGVEQVSAVMATPANLE
                     LLRAAGLMDGAVAASPNDLLIALRGKSAAALETAMTAALDALEKPPAQATGGGPAAEP
                     PRSLQMGVAQVPEANLALVSVPGEYAAAEAMKALRLGLNVMLFSDNVPLAAEISLKKY
                     GREQNLLVMGPDCGTAIIDGIPLGFANAVRRGAIGVVGASGTGTQQVTSLVHRRGAGI
                     SHAIGTGGHDLHQDVGGITMLHGLKLLARDPETRVIVLISKPPAPGVAKQVLDAARRA
                     GKPVVVNFVGAAEAASGANLYPVRTLEEAALAAVALAAGKRPPRPVASDRKIPGMKFR
                     KNQKFIRGLYSGGTFCYEASYLLGDAWSNAPVDPARKMKDAWKSLGDTVVDLGDDVFT
                     RGRPHPMIDHRLRNERLLAEAADPEVAVILFDVVLGYGSHPDPAAEMIPTLLKAKKNR
                     SIAFVGSVCGTDEDPQGLERQEAALRKAGVVLAESNARAVRLAAAIAKRRA"
     gene            2147..3958
                     /gene="atzF"
                     /locus_tag="VIG70_01470"
     CDS             2147..3958
                     /gene="atzF"
                     /locus_tag="VIG70_01470"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HEY8249246.1"
                     /translation="MLQDADPLDLASLRRRTESGALRPSQVVAGVLERIARRGEDSAW
                     IHRLPRAELEARAAELERRGPQGLPLYGIPFAVKDNIDAAGHPTTAACKPFSYVPRES
                     AAVVERLLAAGAILVGKTNLDQFATGLVGVRSPYGVPGNTFDPEYIPGGSSSGSAVTV
                     AAGLVSFSLGTDTAGSGRVPAAFNNLAGLKPTKGLLSTRGVVPACRSLDCVSIFALTA
                     ADAAHVLEVARGFDARDPFSRPASPPARAMPRTFAGCRVGVPREDQLAFFGNPDTPGL
                     FKNFLKKIQDLGAEEVRIDFAPFLETARLLYEGPWVAERYVAIREFIERQPEALHPVT
                     RAIIGGATRHSAIDAFSAYYRLQALRRATAPVWSGIDVMLTPTAGTIYRIAEVEADPV
                     KLNSNLGYYTNFMNLLDLAAIAVPAGFQRDGLPFGVTFAAPAFTDDALCVLGEIAQRA
                     MVKTMGATGLPLPAVDIEETFKDQSLIRLAVCGAHMSGLPLNHQLTERGARLARKCRT
                     APQYRLYALTDFAPPRPGMVRADTGGAIDVEVWDVPATAFGGFVEGIPAPLGIGTVTL
                     EDGQQVKGFLCESHATRSARDITGLGGWRQFIKRGNA"
     gene            3971..5314
                     /locus_tag="VIG70_01475"
     CDS             3971..5314
                     /locus_tag="VIG70_01475"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEY8249247.1"
                     /translation="MSDSASAAHGTDRGFAKLDLELNDYPGAIKTSLFGIQHVLVMFT
                     AMVGGPLIVARLLNLPEETRIAMLSGTMIGCGIATMISALGLGFIGPRLPIVMGVFYI
                     FIGPIVAISKEVSLAAAMTALIIGGLVQFAWSPLIGRLHRFFPPIVTGTTILLIGTGL
                     MKIGINVASGFGTPAFAKPLTIGLAALMIVLILVITRFSTGFIRALSLFITMVIGYFV
                     AAAFGLIDLGSVARADWVTVPKLFPYGGLEWPGLIAVITVVVCFFATAVETTGHTLAV
                     SRIVGVPQEGWRIRGAVANDGIGSSLSAVFGGMALTSYSQNIGVISLTGIGSRFVVAF
                     GGAFLIVMALVPKVGAIIALMPNAVLGGVLLFMFGMVASVGIDIIGRNLTSRRDAVIV
                     AASLALGLGIQAAPPGAFEAIAPALRILVTDGIVMGILLAMFLNLIWPREEEARR"
     gene            complement(5456..6136)
                     /locus_tag="VIG70_01480"
     CDS             complement(5456..6136)
                     /locus_tag="VIG70_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8249248.1"
                     /translation="MKRSSKLAAPVYERLKADIFEFRLMPGTRFSENEVARRARVSRT
                     PVREALFRLEREGYLEVHPKSGWSVRALDFEALDHLYDARVVIECAAVQRLCAGNPRA
                     ALAGLEKAWLVAPRNRLSDGARVARLDEEFHTTLVAAAGNPELARIHHTITERIRIVR
                     RLDFTHPERVVYTYLEHGQILRAILARRSARAVELLRTHIESSKSEVRKISLHRLHLA
                     HLDARQGR"
     gene            complement(6133..>6595)
                     /locus_tag="VIG70_01485"
     CDS             complement(6133..>6595)
                     /locus_tag="VIG70_01485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8249249.1"
                     /translation="APPGTAQLGLHEFQLTAPIGFTFDTSLAIARCIYDGFFDRFPNL
                     KLIASHGGGALPYLVGRLDICWDNIPAARSKTAEPPRNYMRRIYVDSVVFRQDVLNMC
                     VSVCGPDNVLYGSDYPHTIGDMAGCLSRVDALPGEVRHQVRGGNAQRIFKL"
BASE COUNT          935 a         2263 c         2306 g         1091 t
ORIGIN      
        1 tttctccatc cacttcaggc gcgcgatgac gtccggcccg ttggcgccga agcgcagcac
       61 cttgcccagg ccctcgttga gattgctgaa ggtgcggttg ccgtgcgtcg ggttgcgcac
      121 catccacacc ggcatcgacg ggctgatgat gccggccatc gggccgacgg cggcgtggtg
      181 gtggcagggc tcgaacgcga tgtcgccgct ctcggccatg gcgcgcgctt tcgcggcatc
      241 gtccgcccag ccttcgtaca ggattgcgcc gacgatcgcg ccctgcatcg gcccgcacat
      301 gcgcggccac acgatcggcg ggccggcgtg caggatcatg cggctgccca tgcccggcag
      361 gacttcgcgc gcgatgccga tgccctcgag gcgcggctgc gcggcgaggt agcgctccgc
      421 ggcctgcgcg ttggcggctt ccatcttctc gtcgttaacc aggcgcgcga gcgcggcgcc
      481 gaccgcggcg tcgccgttcg ccggcggcgc ccactcgatc cgggtggcgg cgccgccgcg
      541 ctgcacgatg gcgtcggcga acgaggcgag gccgacgttc agcaccgaaa gcggcttggc
      601 gaagagcgcc ctcatgcgcg gcgcttcgcg atcgccgcgg cgagccgcac cgcgcgcgcg
      661 ttgctctcgg cgagcaccac gcccgccttg cgcagtgccg cttcctggcg ctcgaggccc
      721 tgcgggtcct cgtcggtgcc gcacaccgag cccacgaagg cgatcgagcg gttcttcttc
      781 gccttgagca gggtggggat catctcggcc gccgggtcgg gatgcgagcc gtatccgagc
      841 accacgtcga acaggatcac cgcgacctcg gggtcggcgg cttccgccag caggcgctcg
      901 ttgcgcagcc ggtggtcgat catcgggtgc ggccggccgc gcgtgaagac gtcgtcgccg
      961 aggtcgacga cggtgtcgcc gagcgacttc caggcgtcct tcatcttgcg cgccgggtcg
     1021 accggcgcat tcgaccaggc gtcgccgaga aggtaggacg cctcatagca gaacgtgccg
     1081 ccgctgtaga gcccgcggat gaacttctga ttcttcctga atttcattcc cggaattttc
     1141 ctgtcggagg ccacgggacg cggtggccgc ttgccggcgg cgagcgccac cgccgctagc
     1201 gctgcttcct cgagcgtcct gaccgggtag aggttcgcgc cgctcgccgc ttcggcggcg
     1261 ccgacgaaat tcaccaccac cggcttgccg gcgcgccgcg cggcgtcgag cacctgcttt
     1321 gccacgccgg gggccggcgg cttggagatc agcacgatca cgcgcgtttc cgggtcgcgc
     1381 gcgagcagct tcagcccgtg cagcatggtg atcccgccga catcctggtg caggtcgtgg
     1441 ccgccggtgc cgatcgcgtg cgagatgccg gcgccgcgtc ggtgcaccag gctcgtgacc
     1501 tgctgcgtgc ccgtgccgga ggcgcccacc acgccgatcg cgccgcgccg caccgcgttg
     1561 gcgaagccga gcggaatgcc gtcgatgatc gcggtgccgc aatcgggccc catgacgagg
     1621 aggttttgct cccgtccata tttcttcagg gaaatttcag cggcgagcgg cacgttgtcg
     1681 ctgaagagca tgacgttcag gccgaggcgc agcgccttca tcgcctccgc ggcggcgtat
     1741 tcgccgggca cggagacgag cgcgaggttc gcctccggga cctgcgcgac gcccatctgc
     1801 aggctgcgcg gcggctcggc ggcgggcccg ccgccggtgg cttgcgcggg cggcttctcc
     1861 agtgcatcga gcgccgcggt catcgccgtc tcgagcgcag ccgcggattt tccgcgcagc
     1921 gcgatcagca ggtcgttcgg gctcgccgcg accgcgccgt ccatcaggcc ggcggcgcgc
     1981 agcagctcga ggttcgccgg cgtcgccatc accgccgaga cctgctcgac gccgggcagc
     2041 tttccgagcg tcgccgagag ctgcatcagc gacaccgagt cgcggtacag gttcggcaac
     2101 acccggtgga tgtacacaga cttgtatact agagtatgct cgcgcgatgc tgcaagacgc
     2161 tgacccgctc gatctggcgt cgctgagacg gcgcaccgag tcgggggcgc tgcggccctc
     2221 gcaagtggtc gccggcgtgc tggagcgcat cgcgcggcgc ggcgaagact ccgcgtggat
     2281 ccaccgcctg ccgcgcgcag agctcgaggc gcgcgccgcg gagctcgagc gccgcgggcc
     2341 gcaggggctg ccgctctacg gcatcccgtt tgccgtcaaa gacaacatcg acgccgccgg
     2401 gcatcccacg accgcggctt gcaagccgtt tagctatgtc ccccgggaga gcgccgcggt
     2461 cgtcgagcgg ctgctggccg cgggcgcgat cctggtgggc aagaccaacc tcgaccagtt
     2521 cgctaccggg ctcgtcgggg tgcgctcgcc ctacggcgtc ccgggcaaca ccttcgatcc
     2581 cgagtacatc cccggcggct cgagctccgg atcggcggtc acggtcgcgg cgggactcgt
     2641 cagtttcagc ctcggcaccg acacggcggg ctcgggccgc gtgcctgcgg cgttcaacaa
     2701 ccttgccggg ctcaagccga cgaagggcct tctcagcacg cgcggcgtag tgccggcatg
     2761 ccgctcgctc gattgcgttt cgatcttcgc gctcaccgcg gcggacgcgg cccacgtgct
     2821 ggaggtggcg cgcggcttcg acgcgcgcga cccgttctcg cgccccgcgt cgccgccggc
     2881 gcgcgccatg ccgcgaacct tcgccggctg ccgggtgggc gtgccgcgcg aggaccagct
     2941 cgcgttcttc ggcaatcccg acacgcccgg gcttttcaag aattttctga aaaagatcca
     3001 ggacctgggc gccgaagagg tgcgcatcga tttcgcgccg ttcctcgaga ccgcgcgcct
     3061 gctctacgaa ggaccctggg ttgcggagcg ctacgtcgcg atccgtgagt tcatcgagcg
     3121 ccagcccgag gcgctgcacc cggtcacgcg cgccatcatc ggcggcgcca cgcgccacag
     3181 cgcgatcgac gcgttctccg cgtactatcg gctgcaggcg ctgcggcgcg cgaccgcgcc
     3241 ggtgtggagc gggatcgacg tcatgctgac gccgacggcc ggcacgatct accgcatcgc
     3301 cgaagtcgaa gccgacccgg tgaagctcaa cagcaacctc ggctactaca ccaacttcat
     3361 gaacctgctg gatctcgccg cgatcgcggt gccggcgggg ttccagcgcg acgggctgcc
     3421 gttcggggtg acctttgccg cgccggcgtt caccgacgac gcgctctgcg tgctgggcga
     3481 gatcgcgcag cgcgcgatgg tgaagacgat gggggcgacc ggcctgccgc tgccggccgt
     3541 ggacatagaa gaaacgttca aggatcaaag cctgatccgg ctcgcagttt gcggcgccca
     3601 catgtcggga ctgccgctca accaccagct taccgagcgc ggggcgcggc tggcgcggaa
     3661 gtgtcgcacc gcgccgcagt accgcctcta cgcgctcacc gacttcgcgc cgccgcgccc
     3721 gggaatggtg cgcgcggaca ccggcggcgc gatcgacgtc gaggtgtggg acgtgccggc
     3781 gacggcgttc ggcggcttcg tcgagggcat tccggcgccg ctcggaatcg gtacggtgac
     3841 gctcgaggac gggcaacagg tgaaggggtt tctttgcgag agccatgcaa cgcgcagcgc
     3901 tcgcgacatt actggcctgg ggggctggcg gcaattcata aaacggggaa atgcatgacg
     3961 ggaggaacat atgagtgact cggcttcagc cgcgcacggc acggaccggg ggttcgccaa
     4021 gctcgacctc gagctgaacg actacccggg ggcgatcaag acctcgctct tcggcatcca
     4081 gcacgtgctg gtgatgttca ccgcgatggt cggtggcccg ctgatcgtcg cacgactgct
     4141 caatctgccg gaagaaaccc gcatcgccat gttgagcggc accatgatcg gctgcggcat
     4201 tgccaccatg atctcggccc tcggactggg cttcatcggc ccgcgcctgc cgatcgtgat
     4261 gggcgtgttc tacatcttca tcggcccgat cgtcgccatc tcgaaggagg tcagcctggc
     4321 ggcagccatg accgcactga tcatcggcgg gctggtgcag ttcgcctgga gcccgctcat
     4381 tggccggctg caccgcttct tcccgccgat cgtcaccggc accaccatcc tcctgatcgg
     4441 caccggcctg atgaagatcg gcatcaacgt cgcgtcgggg ttcggcacgc cggcgttcgc
     4501 caagccgctg accatcggcc tcgcggcact gatgatcgtg ctgatcctgg tgatcacgcg
     4561 cttctcgacc ggttttatcc gcgcgctgtc gctgttcatc accatggtga tcggctactt
     4621 cgtggcggcg gcgttcggcc tgatcgacct cggcagcgtg gcgagggccg actgggtcac
     4681 ggtgccgaag ctgtttccct acggcgggct ggaatggccc ggcctcatcg cggtgatcac
     4741 ggtcgtggtg tgtttcttcg ccaccgcggt cgagacgacc gggcataccc tcgccgtgtc
     4801 gcgcatcgtg ggcgtgccgc aggaaggctg gcgcatccgc ggcgcggtgg ccaacgacgg
     4861 catcggctcg agcctgtcgg cggtcttcgg cggcatggcg ctcacctcgt actcgcagaa
     4921 catcggcgtg atctcgctca ccggcatcgg cagccgcttc gtggtcgctt tcggcggcgc
     4981 gttcctgatc gtgatggcgc tggttcccaa ggtcggcgcg atcatcgccc tcatgcccaa
     5041 cgcggtgctc ggtggcgtgc tgctgttcat gtttggcatg gtcgcctcgg tcggcatcga
     5101 catcatcggg cgcaacctga cttcgcggcg cgacgcggtc atcgtcgcgg catcgctcgc
     5161 gctcggcctg ggcatccagg cggcgccgcc gggcgccttc gaggcgatcg cgccggcgct
     5221 gcgcatcctg gtcaccgacg gcatcgtcat gggaatcctg ctggcgatgt tcctcaacct
     5281 gatctggccg agggaagaag aggcgcggcg ctagggaagc tgtgaattcg tcgcccccgc
     5341 gaaggcgggg gcccagtttt tctttttgag agcatgggtt cccgcccccg actaaagcac
     5401 tcgggggtga cgttcttgcg cgggaacgac gatcgccact ttttccagag cttccctagc
     5461 ggccttggcg cgcgtcgagg tgcgcgaggt gcagccggtg caggctgatc ttgcgcacct
     5521 cgctcttgct cgattcgatg tgcgtgcgca gcagctcgac cgcgcgcgcc gagcggcgcg
     5581 cgaggatcgc gcgcaggatc tggccgtgct cgaggtaggt gtagaccacg cgctcggggt
     5641 gcgtgaagtc gaggcggcgc acgatgcgga tgcgctcggt gatggtgtgg tggatgcgcg
     5701 cgagctccgg gttgcccgcg gcggcgacca gagtggtgtg gaactcctcg tcgaggcgcg
     5761 ctacacgagc gccgtcgcta agtcgattgc ggggcgcgac cagccaggcc ttctccaggc
     5821 cggccagcgc ggcgcgcggg ttgccggcgc atagccgctg caccgcggcg cactcgatca
     5881 ccacgcgcgc gtcgtagagg tggtccagcg cctcgaagtc gagcgcgcgc acgctccagc
     5941 cgcttttcgg gtgcacctcg agatagcctt cgcgctccag gcggaacagc gcctcgcgca
     6001 ccggcgtgcg cgacacgcgc gcgcgccgcg ccacttcgtt ctcggagaag cgcgtccccg
     6061 gcatcagacg gaactcgaag atgtccgcct tgaggcgctc gtataccgga gcggccagct
     6121 tggaggaacg cttcaaagct tgaaaatccg ctgcgcgttg ccgccgcgca cctgatgcct
     6181 gacctcgccg ggcagcgcgt cgacgcgcga caggcagccc gccatgtcgc cgatggtgtg
     6241 cggatagtcc gagccgtaga gcacgttgtc cgggccgcac accgacacgc acatgttcag
     6301 cacgtcctgg cggaatacca cgctgtcgac gtagatccgg cgcatgtagt tgcgcggcgg
     6361 ctcggcggtc ttcgagcgcg ccgcggggat gttatcccag cagatgtcga ggcgccccac
     6421 caggtagggc agcgcgccgc cgccgtgcga ggctatcagc ttgaggttcg gaaagcggtc
     6481 gaagaagccg tcgtagatgc agcgcgcgat cgcgagcgag gtatcgaagg tgaagccgat
     6541 cggcgcggtg agctggaact cgtgcaggcc gagctgcgcc gttccgggcg gtgcg
//
LOCUS       DATBSZ010000026         8888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1232424, whole genome shotgun sequence.
ACCESSION   DATBSZ010000026 DATBSZ010000000
VERSION     DATBSZ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232424"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /gene="rpsE"
                     /locus_tag="VIG70_01490"
     CDS             complement(<1..347)
                     /gene="rpsE"
                     /locus_tag="VIG70_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY8249250.1"
                     /translation="MRFIAEKIDVGSLELKDQVVSINRVTKVVKGGKNLSFSALVVVG
                     DSAGHVGYGMGKAREVSSAIKKGVEAAKKNIVRVPITDKGSTIPHTVTGRYGSGAVLL
                     KPASEGTGVIAGGA"
     gene            complement(372..737)
                     /gene="rplR"
                     /locus_tag="VIG70_01495"
     CDS             complement(372..737)
                     /gene="rplR"
                     /locus_tag="VIG70_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY8249251.1"
                     /translation="MSTRTKEQTRQRLHDRIRKKIRGSESRPRLAVHRSQAHIYAQVI
                     DDDAGTTLCAASSRDKDVRSKLKSGGNVAAAKEVGGLVASRAKQKGIELVVFDRGGFQ
                     YHGRIKALADAAREAGLKF"
     gene            complement(882..1430)
                     /gene="rplF"
                     /locus_tag="VIG70_01500"
     CDS             complement(882..1430)
                     /gene="rplF"
                     /locus_tag="VIG70_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY8249252.1"
                     /translation="MSRVGRKVIVVPAGVKVQVGDRTLAVQGPKGKLETPVPPGISFA
                     LDGQSLSCLRANDEQRLRAFHGMARALAQNAIKGVTEGFSKDLDIVGVGYRAAVDGKK
                     VVFALGYSHPVEYKVPEGIVIAVEKQTRVTVSGIDRQKVGQVAAEIRGLRRPDPYKQK
                     GIRYVGEQLKKKAGKAGATGAK"
     gene            complement(1441..1848)
                     /gene="rpsH"
                     /locus_tag="VIG70_01505"
     CDS             complement(1441..1848)
                     /gene="rpsH"
                     /locus_tag="VIG70_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY8249253.1"
                     /translation="MNMTDPIADMITRIRNGTRARLPKIDVPASKLKAEIARILKDEG
                     YIANFRLIEDGREGGQGAIRIFLRYGPGWERVITDLQRVSKPGCRIYCGKGEIPRVYG
                     GLGINILSTSHGVMTGRQAAKTGVGGEILCNVW"
     gene            complement(1869..2054)
                     /locus_tag="VIG70_01510"
     CDS             complement(1869..2054)
                     /locus_tag="VIG70_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEY8249254.1"
                     /translation="MAKTSMIAKAKRKPKYAVRAYTRCRLCGRPRAYIRKFEMCRICF
                     RKLALAGEIPGVIKASW"
     gene            complement(2067..2609)
                     /gene="rplE"
                     /locus_tag="VIG70_01515"
     CDS             complement(2067..2609)
                     /gene="rplE"
                     /locus_tag="VIG70_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY8249255.1"
                     /translation="MPRVKEAYQKQVVPRLMQERSYTTVMAVPRLVKVVVNMGIGEAT
                     QNIKLLDAAVDELGRITGQRPATRRAKKSIAAFKLRAGMPIAATVTLRGDRMYEFVDR
                     LFNVALPRVRDFRGVPTNAFDGRGNYTLGLKDQILFPEIDYAKIDKTRGMNVTFVTTA
                     RTDEESKALLAALGMPFRNQ"
     gene            complement(2624..2941)
                     /gene="rplX"
                     /locus_tag="VIG70_01520"
     CDS             complement(2624..2941)
                     /gene="rplX"
                     /locus_tag="VIG70_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY8249256.1"
                     /translation="MRLKKNDLVEVITGRDRGKRGKVLQVLPDKGRVLVQGVNFIKRH
                     TRPNPQRNIKGGIAEREAAIHVSNVMIVSPDDDKRTRIGSKLLPDGERVRIGRRSGEV
                     LDK"
     gene            complement(2981..3355)
                     /gene="rplN"
                     /locus_tag="VIG70_01525"
     CDS             complement(2981..3355)
                     /gene="rplN"
                     /locus_tag="VIG70_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY8249257.1"
                     /translation="MIQMRTILQVADNSGARKISCILPIGGSTGRYARLGDVITANVK
                     ESSPEAPEPVRKGKVVKAVIVRCRKEQRRRDGSYIRFDENAAVLIKEDGEPVGTRVFG
                     PVARELRDKKFMKIVSLAPEVL"
     gene            complement(3376..3687)
                     /gene="rpsQ"
                     /locus_tag="VIG70_01530"
     CDS             complement(3376..3687)
                     /gene="rpsQ"
                     /locus_tag="VIG70_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY8249258.1"
                     /translation="MTDSKPEAANDKPKARVGRVGVVKAAKMQKTVVVRVERLVRHAF
                     YGKTIRRTSTFMAHDELGARAGDRVRIVEARPLSARKRWRVTEILERAVVDAVVEAAR
                     A"
     gene            complement(3684..3878)
                     /gene="rpmC"
                     /locus_tag="VIG70_01535"
     CDS             complement(3684..3878)
                     /gene="rpmC"
                     /locus_tag="VIG70_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY8249259.1"
                     /translation="MKAAKVREMGAEELKTKERQLQEQLFKLRVQQSIGQLDNAIKLR
                     ETRREIARVKTVLREKQVSR"
     gene            complement(3878..4294)
                     /gene="rplP"
                     /locus_tag="VIG70_01540"
     CDS             complement(3878..4294)
                     /gene="rplP"
                     /locus_tag="VIG70_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY8249260.1"
                     /translation="MLMPKKVKYRKQQRGRMSGKAWTGGSIAFGDYGLKALECGWVTA
                     REIEAGRIAITRFIKRNGRIWIRMFPDKPYTKKPQETRMGKGKGAPEGWVMVVKPGRI
                     LFEMEGVPESEAREALRLAAHKLSISTRFVTRAEGA"
     gene            complement(4305..4955)
                     /gene="rpsC"
                     /locus_tag="VIG70_01545"
     CDS             complement(4305..4955)
                     /gene="rpsC"
                     /locus_tag="VIG70_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY8249261.1"
                     /translation="MGQKVHPTGFRLGYTKTWRSRWYAEKEYANLLHEDLALKKDLKK
                     RFAHAGVAKVEIERAANKLKITIHTSRPGIIIGRKGQEVDKLKQEVQKRTGKEVFINI
                     QEILKPELDAQLVSESVALQLEKRIAFRRAMRKAVDAALRFGARGIKIRVSGRLNGAE
                     IARSEWYLHGQLPLHTLRADIDFGFHEAHTTFGQIGVKTWIYRGETDAARPARALA"
     gene            complement(4964..5326)
                     /gene="rplV"
                     /locus_tag="VIG70_01550"
     CDS             complement(4964..5326)
                     /gene="rplV"
                     /locus_tag="VIG70_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY8249262.1"
                     /translation="MASLEAHATNRYVRTSAQKAGLVLALIRGKKAGDALSILRFTKK
                     SVAREVEKTLRSAIANAVLVADRNQKRRLDEEDLVVSAAFADQGPSQKRVRPAPMGRA
                     YRVIKRTTHITVQVAEKE"
     gene            complement(5427..5693)
                     /gene="rpsS"
                     /locus_tag="VIG70_01555"
                     /pseudo
     CDS             complement(5427..5693)
                     /gene="rpsS"
                     /locus_tag="VIG70_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            complement(5696..6517)
                     /gene="rplB"
                     /locus_tag="VIG70_01560"
     CDS             complement(5696..6517)
                     /gene="rplB"
                     /locus_tag="VIG70_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY8249263.1"
                     /translation="MALKQYNPTSPGRRFMTTLDFDDLTKKRPERALTEPLRRTGGRN
                     SKGRITVWFRGGGHKRRYRVVDFRRDKRNVPAKVAALEYDPNRSARLALLHYLDGEKR
                     YILAPEGIKIGQTVVAGEGADILPGNALPLKLIPAGTMIHNLELRAGKGGQLVRTAGG
                     AAQLVAKEADYAQVKLPSGEVRKVHVECMATVGQVGNIDHKNVSFGKAGRKRWLGKKP
                     HNRGVSMNPIDHPHGGGEGRTSGGRHPVTPWGKPTKGAKTRNNKRTQRFIVKRRK"
     gene            complement(6528..6821)
                     /locus_tag="VIG70_01565"
     CDS             complement(6528..6821)
                     /locus_tag="VIG70_01565"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY8249264.1"
                     /translation="MKNLYEVIRRPLLTEKSTLLKESQHTLVFEVHRDATKPEIKKAV
                     ETLFERKVADVRVARVHGKVKRQGRYAGQRPDWKKAFVVLKKGEKPVEFFEQA"
     gene            complement(6826..7650)
                     /gene="rplD"
                     /locus_tag="VIG70_01570"
     CDS             complement(6826..7650)
                     /gene="rplD"
                     /locus_tag="VIG70_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY8249265.1"
                     /translation="MAEKKAKATKRPKAGGPGGAPRNPEKRKKADHASAKGATSSATP
                     EKQSAEKPSAEKPSARSTSPKVAVVNAANETIREIQLSPTVFAAKPGAHLVYEAVKQH
                     RAGARRGTHATKNRALVSGSGRKPWRQKGTGRARVGEIRTPLWRHGGTVFGPQPRDYS
                     FAMPRKARAAALRAALSQRLHEGAVKVVEGFEVPQPKTKLLKVLLATLGISGKAVVVD
                     HQPTEFLVLSGRNLEGVKVVADSHLTAYDVMDCKHLVLSQEAVDRLEERLTPQLRG"
     gene            complement(7653..8279)
                     /gene="rplC"
                     /locus_tag="VIG70_01575"
     CDS             complement(7653..8279)
                     /gene="rplC"
                     /locus_tag="VIG70_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY8249266.1"
                     /translation="MSVEGIIGRKVGMTQVYAEDGQAFPVTVIQAGPCVVVQRKSKDK
                     DGYTAVQLGLVESRKVKHVTKAMKGHFDKAGLPPCRVLREFRVADGAEVKVGDKVSVE
                     QFAPGDSIRVTGTSRGHGFQGVVKRHHFRGGAATHGSMFHRAPGSIGASAFPSRTLKG
                     MRAAGHMGSERVTIKSVRVVRVDASNNLLVVRGSVPGAGGSIVVIRKS"
     gene            complement(8405..8734)
                     /gene="rpsJ"
                     /locus_tag="VIG70_01580"
     CDS             complement(8405..8734)
                     /gene="rpsJ"
                     /locus_tag="VIG70_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY8249267.1"
                     /translation="MLNEKIRIRLKAYDYRILDQSTGEIVDTAKRTGARVAGPIPLPT
                     MKNRWTVNRSPHVDKKSREQFEVRTHRRLLDIFEPTPQTVDALMKLDLPAGVDVEIKA
                     FGKEHQK"
     gene            complement(8748..>8888)
                     /gene="tuf"
                     /locus_tag="VIG70_01585"
     CDS             complement(8748..>8888)
                     /gene="tuf"
                     /locus_tag="VIG70_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY8249268.1"
                     /translation="EMVMPGDNTQVSVELITPIAMEKGLRFAIREGGRTVGSGTVSEI
                     VE"
BASE COUNT         1116 a         3183 c         2886 g         1703 t
ORIGIN      
        1 gccccgcccg cgatcacgcc cgtgccttcg gacgccggct tgagcagcac cgcccccgag
       61 ccgtagcggc cggtgacggt gtgggggatc gtcgatccct tgtccgtgat gggcacgcgc
      121 acgatgttct tcttggccgc ctcgacgccc ttcttgatgg cggaggagac ctcgcgcgcc
      181 ttgcccatgc cgtagccgac gtggccggcg ctgtctccca ccaccaccag ggccgagaag
      241 gacaggttct tgccgccctt gacgaccttg gtcacgcggt tgatcgacac cacctggtcc
      301 ttgagctcga ggctcccgac gtcgatcttc tcggcaatga atctcatttg gctcactctt
      361 ccgcgaacga cctagaactt cagcccggcc tcgcgcgcgg cgtccgcgag ggccttgatc
      421 cggccgtggt actggaagcc gccgcggtcg aacacgacga gctcgatgcc cttctgcttc
      481 gcgcggctcg cgaccaggcc gcccacttcc ttggccgcgg ccacgttgcc gccgctcttc
      541 agcttcgagc gcacgtcctt gtcccgcgac gaggcggcgc aaagcgtcgt gccggcgtcg
      601 tcgtcgatca cctgcgcgta gatgtgcgcc tggctgcggt gcacggcgag ccgcggccgg
      661 ctctcgctgc cgcggatctt cttgcggatg cggtcgtgca gccgctgccg ggtctgttct
      721 ttcgtcctcg tgctcatctc tcgctccggc gggcgttccc gggaggatct ccctcccggc
      781 gtccccgccg ctccttttca atacaggggg tgctgcgccg caccccccgt accccccgct
      841 cgctcacggg agtgaatccc gctcgctcgc aggcttcgag cctacttggc accggtcgcc
      901 ccggccttgc ccgccttctt cttgagctgc tcgccgacgt agcggatgcc cttctgcttg
      961 tacgggtccg gccggcgcag gccacggatc tccgccgcca cctgcccgac cttctggcgg
     1021 tcgatgcccg agaccgtgac gcgcgtctgc ttctcgaccg cgatcacgat cccctcgggg
     1081 accttgtact cgaccgggtg cgagtagccg agggcgaaca ccaccttctt gccatcgacc
     1141 gcggcgcggt agccgactcc gacgatgtcg aggtccttgg agaagccctc ggtcacgccc
     1201 ttgatcgcgt tctgcgccag cgcgcgcgcc atgccgtgga acgcgcgcag ccgctgctcg
     1261 tcgttcgcac gcaggcacga caggctctgc ccgtcgagcg cgaacgagat gccgggcggg
     1321 accggcgtct cgagcttgcc cttgggcccc tgcaccgcga gcgtgcggtc gcctacctgc
     1381 accttgactc cggcgggaac gacgatgact ttgcggccga cccgggacat cgcgcgctcc
     1441 ttaccagacg ttgcagagga tctcgccacc gacgccggtc ttggcggcct gtcggccggt
     1501 catgaccccg tgcgaggtgg acaggatgtt gatgccgagc ccgccgtaga cgcgcgggat
     1561 ctcgcccttg ccgcagtaga tgcggcagcc gggcttgctc acgcgctgca ggtcggtgat
     1621 cacgcgctcc cagccggggc cgtagcgcag gaagatgcgg atcgctccct gaccgccttc
     1681 ccggccgtct tcgatcagcc ggaagttggc gatgtagccc tcgtccttca ggatgcgggc
     1741 gatttcggct ttcagcttgg aagccggcac gtcgatcttg ggcagcctcg cccgcgtccc
     1801 gttccggatg cgggtgatca tgtccgcgat gggatccgtc atgttcatcg catttacctc
     1861 gaaggcttct accagctcgc cttgatcact cccggaatct cgcccgccag ggccagcttc
     1921 cggaagcaga tccggcacat ctcgaacttg cgaatgtagg cgcggggccg tccgcacagg
     1981 cggcaccggg tgtaggcgcg caccgcgtac ttcggcttgc gcttcgcctt ggcgatcatc
     2041 gacgtcttgg ccactcggtc tgcctcctac tggttccgga acggcatccc cagggccgcg
     2101 aggagcgcct tcgactcctc gtcggtccgg gcggtcgtca cgaatgtcac gttcatcccg
     2161 cgggtcttgt cgatcttggc gtagtcgatc tcggggaaca ggatctggtc cttcaggccc
     2221 agcgtgtagt tgccgcggcc gtcgaaggcg ttcgtcggca cgccgcggaa gtcgcgcacg
     2281 cgcggcagcg cgacgttgaa cagccggtcc acgaactcgt acatgcggtc gccgcgcagc
     2341 gtcaccgtcg ccgcgatcgg catgccggcg cgcagcttga acgccgcgat cgacttcttg
     2401 gcgcggcgcg tcgcggggcg ctggccggtg atgcgaccga gctcgtccac cgccgcgtcg
     2461 agcagcttga tgttctgggt ggcctcgccg atgcccatgt tgaccacgac cttgacgagg
     2521 cgcggcacgg ccatcacggt cgtgtagctc cgctcctgca tcaggcgcgg gaccacctgc
     2581 ttctggtacg cctctttcac ccgaggcagg gtcttcgaag cactcacttg tccagcacct
     2641 cgccgctgcg gcggccgatc cgcacgcgct cgccgtccgg cagcagctta gagccgatgc
     2701 gcgtgcgctt gtcgtcgtcg ggcgagacga tcatcacgtt cgacacgtgg atcgcggcct
     2761 cgcgctcggc gatcccgccc ttgatgttgc gctgcgggtt gggccgggtg tggcgcttga
     2821 tgaagttcac gccctgcacc agcacgcgcc ccttgtccgg cagcacctgc agcaccttgc
     2881 cgcgcttgcc gcggtcgcgg ccggtgatga cctccacgag gtcgtttttc ttgagccgga
     2941 tccggatccg gaccggcggc cttctctgtc tcgccatggc ctacagcacc tccggagcca
     3001 gcgacacgat cttcatgaac ttcttgtcgc gcagctctcg cgccactggt ccgaagacgc
     3061 gggtgcccac gggctccccg tcctccttga tcagcaccgc ggcgttctcg tcgaagcgga
     3121 tgtagctgcc gtcgcgccgg cgctgttcct tgcggcagcg cacgatcacg gccttcacca
     3181 ccttgccctt gcgcaccggc tcgggcgcct ccggcgacga ctccttgacg ttggcggtga
     3241 tcacgtcacc gagccgggcg tagcgccccg tcgacccgcc gatcggcagg atgcaggaga
     3301 tcttccgggc gccgctgttg tcggccacct ggaggatggt tcgcatctga atcatgacgc
     3361 gctctccctg ccttcctagg ctcgagccgc ttcgacgacg gcgtcgacga cggcccgctc
     3421 gaggatctcg gtcacgcgcc agcgcttgcg cgccgagagc ggccgggctt ccacgatccg
     3481 cacccggtca ccggccctgg cgccgagctc gtcgtgcgcc atgaaggtgg aggtgcggcg
     3541 gatggtcttg ccgtagaagg cgtggcgcac caggcgctcc accctgacca cgacggtctt
     3601 ctgcatcttg gcggctttca cgaccccgac gcgccccacg cgcgccttcg gcttgtcgtt
     3661 ggccgcttcc ggcttgctgt ccgtcatcgc gacacctgct tctctcgcag caccgtcttg
     3721 acccgcgcga tctcgcgacg ggtctcacgg agcttgatgg cgttgtcgag ctgcccgatc
     3781 gactgctgca cccggagctt gaagagctgc tcctggagct ggcgctcctt ggtcttgagc
     3841 tcttccgctc ccatctcacg cactttggcg gccttcatca ggctccctcc gcccgcgtca
     3901 cgaagcgggt gctgatcgag agcttgtggg ccgcgagccg cagcgcctcg cgcgcttcgg
     3961 actcgggcac gccctccatc tcgaagagga tgcggccggg cttcacgacc atcacccagc
     4021 cctcgggagc gcccttgccc ttgcccattc gcgtctcctg cggcttcttg gtgtagggct
     4081 tgtcggggaa catgcggatc cagatgcggc cgttgcgctt gatgaagcgc gtgatcgcga
     4141 tccggcccgc ctcgatctcg cgcgccgtca cccagccgca ctcgagcgcc ttcagcccgt
     4201 agtcgccgaa cgcgatcgag ccgccggtcc aggccttgcc ggacatgcgc ccgcgctgct
     4261 gcttgcgata cttgaccttc ttcggcatca gcatcggctt acctctaggc gagcgcccgc
     4321 gcggggcgcg cggcgtccgt ctcgccgcgg tagatccagg tcttgacgcc gatctgcccg
     4381 aaggtcgtgt gggcctcgtg gaagccgaag tcgatgtccg cgcgcagcgt gtgcagcggc
     4441 agctggccgt gcaggtacca ctcggagcgg gcgatctcgg cgccgttcag gcggcccgag
     4501 acgcggatct tgatgccgcg cgcgccgaag cgcagcgccg cgtcgaccgc tttgcgcatc
     4561 gcgcgccgga acgcaatccg cttctcgagc tgcagcgcca ccgactcgga gacgagctgc
     4621 gcgtcgagct cgggcttgag gatctcctgg atgttgatga agacctcctt gcccgtgcgc
     4681 ttctgcacct cctgcttgag cttgtcgacc tcctgcccct tgcggccgat gatgatgccc
     4741 ggccgcgagg tgtggatcgt gatcttgagc ttgttggcgg cgcgctcgat ctcgaccttg
     4801 gcgacgccgg cgtgggcgaa gcgcttcttc aggtccttct tgagcgccag gtcctcgtgc
     4861 agcaggttcg cgtattcctt ctccgcgtac cagcggctgc gccaggtctt ggtgtaaccg
     4921 agccggaacc cggtgggatg gactttctga cccataggtc ctgctactcc ttttccgcga
     4981 cctggacggt gatgtgcgtg gtgcgcttga tgacgcggta ggcgcggccc atcggcgcgg
     5041 gccggacgcg cttctgcgac gggccctggt cggcgaaggc cgcgctcacg acgaggtcct
     5101 cctcgtcgag ccttcgcttc tggttgcggt cggccaccag caccgcgttt gcgatcgccg
     5161 agcgcagcgt cttctcgacc tcgcgcgcca ccgacttctt ggtgaagcgc aggatgctga
     5221 gcgcgtcgcc ggccttcttg ccgcggatga gcgccagcac cagccccgcc ttctgggcgg
     5281 aggtccgcac gtaccggttg gtggcgtggg cttcgagcga tgccatctcc gcctccctag
     5341 ctcttgcccg ccggcggggc ggccgcgggc gccgccgcgg cgcccggcgc cccgggcctg
     5401 ccgggcgctg ccgtcgaagt cgccaccttc tccaccttcg aggagtggcc cttgaactgg
     5461 cgcgtgggcg agaactcgcc gagcttgtgc ccgaccatgt tctcggtcac gtagaccggg
     5521 acgaacttct tgccgttgta gacggcgagc gtgtggccga ccatctcggg gatgaccgtc
     5581 gagcggcgcg accaggtctt gatgaccttc ttgtcgcccg ccgagttcat cgcctccacc
     5641 ttcttgagca ggtggtcgtc cacgaacggg cccttcttca gcgaccttgc catggctact
     5701 tccgcctctt cacgatgaag cgctgcgttc tcttgttatt gcgcgtcttg gcgcccttcg
     5761 tcggcttgcc ccacggggtc accgggtggc gtccgccgga ggtgcggccc tcgccgccgc
     5821 cgtgcgggtg gtcgatcggg ttcatcgaga cgccgcggtt gtgcggcttc ttgccgagcc
     5881 agcgcttgcg ccccgccttg ccgaacgaca cgttcttgtg gtcgatgttg ccgacctggc
     5941 cgacggtcgc catgcactcg acgtgcacct tgcgcacctc gcccgacggc agcttcacct
     6001 gggcgtagtc ggcttccttc gccaccagct gcgccgcgcc gccggccgtg cgcacgagct
     6061 gtccgccctt gccggcccgc agctcgaggt tgtggatcat ggtgccggcc gggatcagct
     6121 tcagcggcaa ggcgttgccg ggcaggatgt ccgcgccctc gccggcaacc acggtctggc
     6181 cgatcttgat gccctcgggc gccaggatgt agcgtttctc gccgtccagg tagtgcagca
     6241 gcgccagccg cgccgagcgg ttggggtcgt actcgagcgc cgccaccttg gcgggcacgt
     6301 tgcgcttgtc gcggcggaag tccaccacgc ggtagcggcg cttgtgcccg ccgcccctga
     6361 accaaaccgt gatccgaccc ttgctgttgc ggccgccggt gcggcgcaag ggttcggtga
     6421 gcgcgcgctc ggggcgcttc ttcgtcaggt cgtcgaagtc gagcgtcgtc atgaagcgac
     6481 gaccgggcga cgtcgggttg tactgcttca gtgccatgtc gtttccccta cgcctgctcg
     6541 aagaactcga cgggcttctc gcccttcttg aggacgacga aggccttctt ccagtcgggc
     6601 cgctggccgg cgtagcgtcc ctgccgcttc accttgccgt gcacccgcgc cacgcggacg
     6661 tcggccacct tgcgctcgaa gagcgtctcg acggccttct tgatctcggg cttggtcgcg
     6721 tcgcggtgca cctcgaagac gagcgtgtgc tgcgactcct tgagcagggt ggacttctcg
     6781 gtcaagagcg gccggcggat cacttcgtag aggttcttca tcgccctatc cccgcagctg
     6841 cggcgtgagc cgctcctcga gcctgtcgac ggcctcctgg ctcagcacca ggtgcttgca
     6901 gtccatcacg tcgtaggcgg tgaggtggct gtccgcgacg accttcacgc cctcgaggtt
     6961 gcggcccgac agcaccagga actcggtggg ctggtggtcg acgaccaccg ccttgcccga
     7021 aatacccagc gtcgcgagca gcaccttgag cagcttggtc ttcggctgtg gaacctcgaa
     7081 gccctcgacc accttgacgg caccctcgtg caggcgctgg ctgagcgccg cgcgcagcgc
     7141 ggcggcgcgc gccttcctcg gcatcgcgaa cgagtagtcg cgcggctgcg gcccgaacac
     7201 cgtgccgccg tggcgccaga gcggggtgcg aatctcgccg acgcgcgcgc ggcccgtgcc
     7261 cttctgccgc cagggcttcc tgcccgagcc cgagacgagc gcccggttct tggtcgcgtg
     7321 cgtgccgcgg cgggcgcctg cgcggtgctg cttgaccgcc tcgtaaacga ggtgcgcgcc
     7381 gggcttcgcg gcgaacacgg tcggcgagag ctggatctcg cggatcgtct cgttcgcggc
     7441 gttgacgacg gccaccttgg gagacgtcga ccgtgccgat ggcttctcgg cggagggttt
     7501 ctcggcggac tgcttttcag gagtggcgct cgaagtcgcg cccttggcgg aggcgtgatc
     7561 agccttcttc cgcttttccg ggttgcgcgg cgcgcccccc ggacccccag ccttcggccg
     7621 cttcgtcgct ttcgccttct tctccgccat ggctagctct tcctgatcac gacgatcgag
     7681 ccgcccgcgc ccggcaccga gccgcgcacg accagcaggt tgttcgaagc gtcgacgcgc
     7741 accacccgga cgctcttgat cgtgacccgc tcgctgccca tgtgcccggc ggcgcgcatc
     7801 cccttgagcg tgcgggacgg gaacgccgag gcgccgatcg agcccggggc gcggtggaac
     7861 atcgagccgt gggtcgcggc gccgccgcgg aagtggtggc gcttcacgac gccctggaag
     7921 ccgtggccgc ggctcgtgcc ggtcacgcga atcgagtctc cgggggcgaa ctgctcgacc
     7981 gagaccttgt cgccgacctt gacctcggcg ccgtccgcca cgcggaactc gcgcagcacg
     8041 cggcaggggg gaagcccggc cttgtcgaag tgacccttca tcgccttggt gacgtgcttg
     8101 accttgcgcg actcgacgag gcccagctgc acggcggtgt agccgtcctt gtccttggac
     8161 ttgcgctgga cgacgacaca cgggcccgcc tggatgaccg tgaccgggaa cgcctgtccg
     8221 tcttccgcgt agacctgggt catgccgacc ttgcgtccga tgattccttc gactgacatc
     8281 tcgctcgttg ccttcaatct ttctcaaggc ttacagcttt gcgaggtcgc cgcctggcgt
     8341 tgcctctcgc tgcgttgcct tgcgccgggg ggcgctcatc gcccccccgg cacccccccg
     8401 atgactattt ctggtgctcc ttgccgaacg ccttgatctc tacgtcgacg cccgcgggca
     8461 ggtcgagctt catgagcgcg tcgaccgtct gcggcgtcgg ctcgaagatg tcgagcagcc
     8521 gccggtgcgt gcggacctcg aactgctcgc ggctcttctt gtcgacgtgc ggcgagcggt
     8581 tcaccgtcca gcggttcttc atcgtcggca gcgggatcgg cccagccacg cgagcgcccg
     8641 tgcgcttcgc ggtgtccacg atctcgcccg tcgactggtc gaggatccgg tagtcgtacg
     8701 ccttcagccg gatccggatc ttttcgttca gcatcatgtg aagccgccta ctccacgatc
     8761 tcgctgacgg tgcccgagcc gacggtgcgg ccgccctcgc ggatcgcgaa gcgcagcccc
     8821 ttctccatcg cgatcggcgt gatcagctcc accgacacct gcgtgttgtc ccccggcatc
     8881 accatctc
//
LOCUS       DATBSZ010000027        13220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2325327, whole genome shotgun sequence.
ACCESSION   DATBSZ010000027 DATBSZ010000000
VERSION     DATBSZ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13220
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2325327"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(550..1275)
                     /locus_tag="VIG70_01590"
     CDS             complement(550..1275)
                     /locus_tag="VIG70_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8249269.1"
                     /translation="MSNTFRNTFTRKEFALAVACALALGVFSEIANGQVDANENALVV
                     DSSGAPVMSGFGLCVRNGFGPAPSWTVACHGAAPVPAVVYYYPPAAAPAATTAPAPFP
                     VAMAAAAPLPVWEKVAFDANVLFDSDQSALRPAGRVTLDEFVANIHGLESQNILAIGY
                     SDRMGSHASNQALSERRVDAVKAYLVGKGIKAERVQTSARGETRPSTFAGECRDANNT
                     KNVACMQPDRHVFIEVSGTRVAK"
     gene            1461..2204
                     /locus_tag="VIG70_01595"
     CDS             1461..2204
                     /locus_tag="VIG70_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8249270.1"
                     /translation="MGATHPASIPLKANRLLAALPDDGLRALQPFLEAVPLPLGMVVY
                     ESGGTQGYVYFPTSSIVSLLYVLADGASAEIAVTGNEGLVGIALFMGGETTPSRAVVQ
                     SAGHGYRMRAAVLKKAFEAGGDLQHLLLRYTQALITQMTQTAVCNRHHSVDQQLCRWL
                     LLSLDRLPGNELVMTQELIANMLGVRREGVTEAAGKLQAEGLIHYSRGRITVPDRRQL
                     EARVCECYAVVKKEYDRLLPAALSQPLAH"
     gene            complement(2184..2864)
                     /locus_tag="VIG70_01600"
     CDS             complement(2184..2864)
                     /locus_tag="VIG70_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8249271.1"
                     /translation="MRSALAAVLVLAAWSAGAQPAAPAEVRPDVLMSGVTTEVIAVLK
                     HDAAAGQPTDVVRLVETKILPLFDFRHMTRIAVARNWRLASPEQQAALVVQFRTLLVR
                     TYSSALSTYRDEEIEYKPLRFAPGETDVLVRSAVRRRGAETLTLDYDMESTEAGWKVY
                     DVKIAGVSMVLAYRETFAEAVRAGGIDGLIKQLSDKNGQNESRPKGAGPQTLLAPVLV
                     LRLVRERL"
     gene            complement(2876..3187)
                     /locus_tag="VIG70_01605"
     CDS             complement(2876..3187)
                     /locus_tag="VIG70_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8249272.1"
                     /translation="MSFLKRSTAFFAAASLLSVLGCAATSTQESTGEYIDDSVITSKV
                     KTAILNEPSLKVFQIGVDTNKNVVQLSGSVATGAERAKATEIARSVPGVRSVKNDLLL
                     K"
     gene            3364..4101
                     /locus_tag="VIG70_01610"
     CDS             3364..4101
                     /locus_tag="VIG70_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006480091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8249273.1"
                     /translation="MQARRTPKQNHLLAALPAAELMRLAPHLEPVPLPLGAVIYESGG
                     AQDHVYFPASGIVSLLYVMNDGSAAEIAVVGNEGMVGIALFMGGGTTPSRAVVQSAGS
                     AWRLDAARIKAEFARGGALQRLLLRYTQALITQMTQTAVCNRHHVLEQQLCRWLLLSL
                     DRLPSNRLVMTQQLIANMLGVRREGVTEAAGRLQAEGLIDYRRGRICVLDRAKLEKRV
                     CECYGVVRRECDRLFAPAPALVAALAA"
     gene            4101..4805
                     /locus_tag="VIG70_01615"
     CDS             4101..4805
                     /locus_tag="VIG70_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8249274.1"
                     /translation="MAAAIQNSLLAALPRKSYLRLAPGLAPVELVFGEVLYEPGDPIR
                     DVYFPTQSLVSLLTVVEGHLALEVGMVGREGMVGVPLVLGADASPVRALVQGAGPALK
                     MDAQRFRNELNASPPLRREVQRYIYAMMVQISQTAGCNRFHVVEARLARWLLMTRDRM
                     RSGHFRMTHEFLSHMLGVRRVGVTEAASALQRRKLIEYSRGSITILDDRGLEAACCSC
                     YQVVNDMHETERETAA"
     gene            complement(4846..5859)
                     /locus_tag="VIG70_01620"
     CDS             complement(4846..5859)
                     /locus_tag="VIG70_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEY8249275.1"
                     /translation="MNRRTLALLGAALLPATSGAIEGLARCAKLENADERIACYDELA
                     RATEAPGGGPIEASPTPSHLSEAWKLGAKHGDARRLTDILGYRPTYIISRWTSDPNEQ
                     PSSPAPDGDSLEPQDLDANEIKFQISFKVELVSRQAFDQLGVTPFLGHIGINSVRLWF
                     AYTHLVNWQMYNTDASRPFRETNYEPEAILSFGTGNEGDGFKLVNLGLMHQSNGQTEA
                     RSRGWNRVYAQGGWEWDRVSVLARLWYRLPDSAEDDDNPDITHYLGNGDIVTRYQTDR
                     GYVSSLLVRRRFVQLDWATPILAALGAARLHLQLTSGYGETLIDYNHKQTTLGVGVSF
                     GNW"
     gene            complement(5859..6599)
                     /gene="gspN"
                     /locus_tag="VIG70_01625"
     CDS             complement(5859..6599)
                     /gene="gspN"
                     /locus_tag="VIG70_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEY8249276.1"
                     /translation="MRRWSAAAFGLGAFAVALLALAPATLIDAQLARASDGRLRLASA
                     QGSLWAGTGWLEIRDAAGRAGVAKPLAWRVRPASLLRGRFVAEVDLDQTAKPFPVTIS
                     LSQVEIADAGIRLPAAALGLGMPKLAPFRLTGDVLVKIPRLSVERGRADGEATLQWRA
                     AGSALAPISPLGEYEVHLKAVGPTVHAALRTVEGPLQLEGTGSWANGAAPNFLATAHV
                     PEQHRDQLVPLLRLFAVQRGAGAFELQL"
     gene            complement(6596..7087)
                     /gene="gspM"
                     /locus_tag="VIG70_01630"
     CDS             complement(6596..7087)
                     /gene="gspM"
                     /locus_tag="VIG70_01630"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEY8249277.1"
                     /translation="MKAALRRAWESRAPRERIVIAVAAAVLGVASYVLVVHSAERGRR
                     QLSASVDTLRTQAAILDQQATELARLRATPAPAVSPTDLRALVQARVDAAGLSRALTR
                     LDAPDADHVRVTLGAVSVADWLGWIAALQSQNVRLESARVEALSAPGMVGATATLTRA
                     RAP"
     gene            complement(7084..8250)
                     /gene="gspL"
                     /locus_tag="VIG70_01635"
     CDS             complement(7084..8250)
                     /gene="gspL"
                     /locus_tag="VIG70_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HEY8249278.1"
                     /translation="MSLLRIYAPLGDAPLRCEWALIHESEVVRGEGPLAELPRGAGRV
                     QLVIPAAQVLITRAELPPSARRNGGSVLAFALEDVTAAEPEANQVSWLGADAFAALDK
                     AALKRWRDALRAAGLRAAEVHSEILLLPRANREWSLAWDGAEGFVRTGEFEGTATDSG
                     DAASPPLELRLLLERSRPDSLAVYGTAPDLAAWQRELGIPLYAAGAWDWRTAPEQAGV
                     SLAQERRRWRIAPGALASLRPAAWIAGAALALHGTALVTDWMRLAAEQRSVRAGMEAR
                     FRSAFPDAVAVADPALQMRRQLAAARHRAGVPDAGDFAPMIEKVALGLKELPAGGLRT
                     VSYESGRMTLELAAIDEPALRRTVARLTQTGLIADVAAVDTKNLGGKLITVRAP"
     gene            complement(8247..9164)
                     /gene="gspK"
                     /locus_tag="VIG70_01640"
     CDS             complement(8247..9164)
                     /gene="gspK"
                     /locus_tag="VIG70_01640"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEY8249279.1"
                     /translation="MNALRQRGVAIVLALGVVAFAAIAAAAILASQSTWSRQAELGAE
                     HTQAQALVQAGIDWGRALLSDDRRLSNIDHPGEPWALRLPPVPVDNGELAGQIEEQQG
                     LFNLNNLVRSGKVSPAHVAQFRRLLEILALPDTLAASVADWLDADGEPQPQDGAESDY
                     YQGLQPPYLAANRPLGDVAELALVRGFDEAARKRLRPFVTALPRFTPVNVNTASPEVL
                     AAVVSNLDLDQARALTAQRGRAYFRTLPEFLAQLPSGAVADGNDVTTASQYFVASARV
                     TIGGAQARGSALLERESTGWPTVVWRKYP"
     gene            complement(9161..9694)
                     /locus_tag="VIG70_01645"
     CDS             complement(9161..9694)
                     /locus_tag="VIG70_01645"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HEY8249280.1"
                     /translation="MRPRGFTLIELMIALAILSLLALMSFRGLGTVLDARDHVRQETE
                     KWKRVAAFYSRFQRDVQLSAPREVRAAAGRFEFTRFATSEGIDAPRRVAYRLNENHEI
                     EITLWPGLDVAPNAEPTRYAVLPAVAQFELQYLDGNLAWAGAWPRSQRDPPLPRAVRL
                     RLVLASGEELVRVFSLR"
     gene            complement(9691..10074)
                     /gene="gspI"
                     /locus_tag="VIG70_01650"
     CDS             complement(9691..10074)
                     /gene="gspI"
                     /locus_tag="VIG70_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEY8249281.1"
                     /translation="MNRSRWSFAAGFTLVEVLVALAIVSIALMAALRAAGQGTSAVSE
                     LRLRMLAGWVAENRLAEHRARGDWLPLGIARGTESQGGVELAWREEVIATPNSAFRRV
                     DVFVSQPAQESRSLARLTGFVAQPR"
     gene            complement(10061..10567)
                     /locus_tag="VIG70_01655"
     CDS             complement(10061..10567)
                     /locus_tag="VIG70_01655"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY8249282.1"
                     /translation="MPISAPGASRGFTPPHKCGFTLLELLVVLLIIGLFAGLVATITR
                     PDERALLRLEAERLAQLLDLATVESRLTGRPVAWTADAARYQFWRWREDTGWIEVQDD
                     ALRARSLPPGMAISDLRIETMRPPGSMRLEFAPEGSLAYEFQMSLGAARYSVAASPLG
                     EVRIHEPQ"
     gene            complement(10539..10955)
                     /gene="gspG"
                     /locus_tag="VIG70_01660"
     CDS             complement(10539..10955)
                     /gene="gspG"
                     /locus_tag="VIG70_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006580210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEY8249283.1"
                     /translation="MQRGFTLLEVMVVVVILAILAALVVPKIISRPDEARAIAAKQDI
                     ASLMQALKLYRLDNQRYPTTEQGLQALVARPASAPPAPNWKAGGYVERLPKDPWGNPY
                     QYLNPGVHGELDVSSMGGDGAPGGEGSDADIGSWSL"
     gene            11098..11478
                     /locus_tag="VIG70_01665"
     CDS             11098..11478
                     /locus_tag="VIG70_01665"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEY8249284.1"
                     /translation="MAAASLLGVVAAYWSWAFLAPAPTPRAPPLADFAGSSSSARGMF
                     GSVKDGAAPAASSAVRLTGVVAASDGRRGHAVLRLDGKRTVAVLQGEDVEPGLRLAEV
                     HADRIVLERNGARETLTWPEKGGK"
     gene            11508..>13220
                     /gene="gspD"
                     /locus_tag="VIG70_01670"
     CDS             11508..>13220
                     /gene="gspD"
                     /locus_tag="VIG70_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEY8249285.1"
                     /translation="MAPLQAQAQAQAPAPDTPLQAQAPDTVTLNFLNADIEGVVKAVS
                     EITGKNFVLDPRVKGTINIVSARPVPRALVYDVFLSALRLQGYAAVEDRGVVKIVPEA
                     EAKLHPGRGQVQTRVFTLKYESAAQLVPVLRPLIPPANTIAAYPGSNALVITDYADNL
                     QRLARIIDSIDQPGGTDPVVIPLAHASALDVAQMVNRVFAEAPQAAAPGADTSQRLSV
                     VADARSNSLVVRADNPSRIARLRTLVAMLDSPTSAAGNLHVVYLKNAEAVKVAETLRA
                     IYLGETVAAAAPRPTTASAMTPASTSPVGAPLAALPAPQAVALVPGMIQADPATNSIL
                     IHAPDTIYKNLRAALDKLDVRRAQVYVEALIAEITADKAAEFGVQWQSLSGVEKSGTN
                     AFGGTNFGGPGQNVLGIAANPATAGRGLNVGVINGVVNIPGIGEILNLNLLIRALESD
                     SNANILSTPTLLTLDNEEARIIIGQNVPFITGSYAVTGAATTPTPFQTVERKDVGLTL
                     RVKPQISEGGTVRLQIYQEVSSVVDTILPNPAGVTTNKRAVESTVLVDDGQIVVIGGL
                     IQDGV"
BASE COUNT         2051 a         4556 c         4596 g         2017 t
ORIGIN      
        1 cgcgtcccgc accgtgaagc cgcagagggc ggggcagcgg cagaagagcg cgcggatgct
       61 ggcctcgaga tccgcctcga agcggctgcg ttgcaggtcc tcgatccgat gctcttgcgt
      121 tttcatgtcg gcagcgtctc agggatcgtg cgcgctttgg cggcgggtgt atgttcgctt
      181 tcgaacaaat gcgcccgtct gttactggta ccgctcgcgg cgctcgccga gagcgcgagc
      241 ctcaccgtgt catatgttcc tgaacgcgac cagcagcagc agcacgccca atccaatggc
      301 tgccgcgctc aagatgatcg gcacgtagac ggtttccttt tccttcaccc ggagcacgac
      361 cgagccgagc ttcgcgctgt gatccttgcg atgcccatgg tagtccagaa aaaaaggggc
      421 ccgggacggg gggagccgtt gccgggccgg ggggtgctca agggggggac acgcaccccc
      481 tgcgccatca ttcatctttt ccgcgacggg ttctgtgcgg tcccgaacat aagccgggcc
      541 gcccttcgac tacttcgcga ccctggtccc ggatacttcg atgaagacgt gacggtcggg
      601 ttgcatgcag gcgacgttct tcgtgttgtt ggcgtccctg cattcaccgg cgaaggtgct
      661 gggccgcgtt tcaccccggg cgctggtctg cacgcgctcc gccttgattc ccttgccgac
      721 cagataggct ttcacggcgt cgacgcgccg ctctgaaagg gcctggttgg aagcatgact
      781 ccccatgcgg tcggaatacc cgatcgccag gatgttttgc gactcgaggc cgtggatgtt
      841 ggcgacgaac tcgtcgaggg tgacgcggcc ggccggacgc agcgccgatt gatccgagtc
      901 gaacaggaca ttggcgtcga aggcgacttt ttcccacacc ggcagcgggg ccgccgcggc
      961 catcgccacg gggaaggggg ccggggccgt cgttgcggcg ggcgcggcag cgggcgggta
     1021 gtagtagacc acggcgggca ccggggcggc gccatggcat gcgaccgtcc acgacggggc
     1081 cgggccgaaa ccgttgcgca cgcaaaggcc gaagccgctc atgacgggcg cgccgctcga
     1141 atcgacgacc agggcattct cgttggcgtc cacttggccg ttggcgattt cggaaaagac
     1201 gccgagcgcc agcgcgcagg cgacggccag ggcgaattcc ttccgggtga aggtgtttct
     1261 gaaggtgttg ctcatgatgt tctctcgatg actgccttgc agtggctcag aaaaaagata
     1321 ctgagtgatc actcatgcag tgtcggcctt tcttcgtcgg ccatctgcct gctggcacac
     1381 atagcgtctg tgcgctggcg tactaaacgc agcgaccttc agtgttcgat accgtacaaa
     1441 aggcgtatga tggacaccca atgggcgcaa cccatccagc atcgattccg ctgaaagcca
     1501 atcgccttct cgccgccctg cccgacgacg gcctgcgggc gctgcagccc tttctggagg
     1561 cggtgccgct gccgctcggc atggtcgtgt atgagtctgg cggcacccag ggctacgtgt
     1621 acttcccgac gagcagcatc gtgtcgctcc tgtacgtgct ggcggacggt gcctcggcgg
     1681 agatcgccgt taccggcaac gagggcctgg tgggcatcgc gctcttcatg ggcggcgaga
     1741 ccaccccgag ccgcgcggta gtgcagagcg ccggacacgg ctaccggatg cgggccgcgg
     1801 tgctgaagaa ggcgttcgag gccggcggcg acctgcagca cctgctgctg cgctacacgc
     1861 aggcgctgat cacgcagatg acgcagacgg cggtctgcaa tcgccatcac tccgtcgacc
     1921 agcagctttg ccgctggctg ctgttgagcc tggaccggct tcccggcaac gagctggtga
     1981 tgacgcagga actgatcgcc aacatgctcg gggtgcgccg cgaaggcgtc accgaggccg
     2041 ccgggaagct gcaggccgag gggttgatcc attacagccg cggccgcatt accgttcccg
     2101 accgccggca gctcgaagcg cgcgtgtgcg agtgctacgc ggtggtcaag aaggaatacg
     2161 accggctgct gccggccgcc ctctcacagc cgctcgcgca ctagccgcag caccaggacc
     2221 ggtgcgagca gggtctgcgg gccggccccc ttggggcgcg attcgttctg cccgttcttg
     2281 tccgagagct gcttgataag accgtcgata ccgccggcgc gaaccgcctc ggcgaacgtt
     2341 tcacgatagg cgaggaccat gctgaccccg gcgatcttga cgtcgtagac cttccagccc
     2401 gcctcggtgc tctccatgtc gtagtcgagg gtcagggtct cggccccgcg ccgccgcacc
     2461 gcggatctca ccagcacgtc ggtttcgccg ggcgcgaagc gcaaaggctt gtactcgatt
     2521 tcctcgtcgc ggtaggtcga gagcgccgag gaataggtgc gcaccagcag cgtcctgaat
     2581 tgcacgacca gcgccgcctg ctgctccggc gaggcgaggc gccagttgcg cgccacggcg
     2641 atgcgggtca tgtgccggaa atcgaacagc ggcaggatct tggtctcgac cagccgcacc
     2701 acatcggtgg gctgaccggc cgcggcgtcg tgcttgagaa cggcgatcac ctccgtggtg
     2761 accccgctca tcagcacatc ggggcgcacc tcggcaggcg ccgcgggctg cgccccggcg
     2821 ctccaggcgg cgaggaccag gacggcggcc agggcgcttc gcatcgagcg cacggctact
     2881 tgagcagcag gtcgttcttg accgagcgca cgcccgggac cgagcgggcg atctcggtgg
     2941 ccttggccct ctccgcgccg gtcgcgaccg agccgctcag ctgcaccacg ttcttattgg
     3001 tgtcgacgcc gatctggaac accttcagcg aaggctcgtt gaggatcgcg gtcttcacct
     3061 tcgacgtgat cacgctgtcg tcgatgtatt cgcctgtgct ttcctgggtg gacgtcgcgg
     3121 cgcagcccag cactgacagc agcgaggcgg cggcgaagaa ggcggtggag cgcttgagga
     3181 agctcatgag gatctcgatt cgtatcgatg agacggttgg cttgcggaga aactgctgca
     3241 cgcattgtgt gcatccgggg gcgccggttc cgtgcgccag cgcactgtct gcgggccgtc
     3301 gcagccgcca tacgtgggcg agcgtacata ccgtgcgcga ttcagcttgg acaatgaagt
     3361 ccgatgcaag cgcgccgcac cccgaaacaa aaccatctcc tcgccgcgct gccggcggcg
     3421 gagctcatgc ggctcgcgcc gcatctcgag ccggtgcccc tgccgctggg cgcggtgatc
     3481 tacgaatcag gcggcgcgca ggaccacgtg tattttccgg cgagcggcat cgtgtcgctg
     3541 ctctacgtaa tgaacgacgg ctccgccgcg gagatcgcgg tggtgggcaa cgagggcatg
     3601 gtgggcatcg cgctgttcat gggcggcggc acgaccccga gccgcgcggt ggtgcagagc
     3661 gcaggcagcg cctggcggct cgacgcggcg cggatcaagg ccgagttcgc ccgcggcggc
     3721 gcgctgcagc gcctgctcct gcgctacacg caggcgctga tcacgcagat gacgcagacc
     3781 gcggtgtgca accgccacca cgtcctcgag cagcagcttt gccgctggct gctgctgagc
     3841 ctggaccggc tgccgtcgaa ccggctcgtc atgacgcagc agctcatcgc caacatgctc
     3901 ggcgtgcgcc gcgaaggggt caccgaggcc gcgggccggc tgcaggccga gggcctcatc
     3961 gactatcgcc gcggccgcat ctgcgtgctc gaccgcgcga agctcgagaa acgcgtatgc
     4021 gagtgctatg gcgtggtgcg gcgcgagtgc gaccggctgt ttgcgccggc ccccgcgctg
     4081 gttgccgcat tggctgcctg atggctgccg cgatccagaa cagcctgctc gccgctctgc
     4141 cgcgcaagtc ctacctgcgc ctcgcccccg ggcttgcgcc cgtcgagctg gtcttcggcg
     4201 aggtgctcta cgaacccggc gatccgatcc gggacgtgta ctttcccacc cagtcgctgg
     4261 tctcgctgct cacggtggtg gaaggtcacc tcgcgctcga ggtcggaatg gtgggccgcg
     4321 agggcatggt gggcgttccg ctggtactcg gcgccgacgc ttcgccggtg cgcgcgctgg
     4381 tgcagggcgc cggcccggcc ctgaagatgg acgcgcagcg cttccgcaac gagctgaacg
     4441 cgagcccgcc gctgcggcgc gaggtgcagc gctacatcta cgcgatgatg gtgcagatct
     4501 cgcaaaccgc gggctgcaac cgcttccacg tcgtggaagc gcggctcgcc cgctggctgc
     4561 tgatgacccg cgaccggatg cgctcgggac atttccgcat gacgcacgag tttctctcgc
     4621 acatgctcgg cgtgcgcagg gtcggggtca ccgaagcggc gtccgcgctg cagcggcgca
     4681 agctgatcga gtacagccgc ggcagcatca ccatcctgga cgaccgcggg ctcgaggcgg
     4741 cctgctgcag ctgctatcag gtcgtcaacg acatgcacga aacggagcgc gaaacggccg
     4801 cctgaagaaa aagcccggtc ctcctggcac aagccggcat ttgccttacc agttgccgaa
     4861 ggacacgccg acgccgaggg tggtctgctt gtggttgtaa tcgatcaggg tttcgccgta
     4921 acccgaggtc agctgcaggt ggagccgtgc ggcgccgagc gcagccagga tcggcgttgc
     4981 ccagtcaagc tgcacgaatc tgcggcgcac gagcagagag ctcacgtagc cgcgatccgt
     5041 ctggtagcgg gtgacgatgt cgccgttacc caggtagtgc gtgatatccg ggttgtcgtc
     5101 atcctcggcg ctgtccggga gccgatacca gaggcgcgcg agcaccgaga cccggtccca
     5161 ttcccagccg ccctgcgcgt acacccggtt ccagccgcgc gagcgagctt cggtttgccc
     5221 gttcgattgg tgcattaggc cgaggttgac gagcttgaag ccgtcgcctt cgttgccggt
     5281 gccgaagctg aggatcgcct cgggctcgta gttggtttcc cggaaagggc gcgaggcgtc
     5341 ggtgttgtac atctgccagt tcacgaggtg cgtataggcg aaccacaagc gcacgctgtt
     5401 gatgccgatg tgaccgagaa acggggtgac gccgagctgg tcgaatgcct ggcgcgaaac
     5461 gagctccacc ttgaagctga tctggaactt gatctcgttg gcgtcgaggt cctgcggctc
     5521 gagcgagtcg ccgtccggcg ccgggctgct cggctgctcg ttagggtcgc tcgtccagcg
     5581 cgaaatgatg taggtcgggc ggtatcccag gatgtcggtc aggcgccgtg cgtcgccgtg
     5641 cttcgcgccg agcttccacg cctcgctcag gtgcgaagga gtgggcgagg cctcgatcgg
     5701 cccgccgccg ggcgcttcgg tcgccctcgc cagctcgtcg tagcaggcga tccgctcgtc
     5761 ggcgttctcg agcttcgcgc agcgcgcaag gccttcgatc gcgccgctcg tcgccggcag
     5821 cagcgccgcg cccagcagcg cgagcgtgcg gcgattcact acagctgcag ctcaaaagcg
     5881 cccgcgccgc gctggacggc gaaaaggcgc agaagcggca cgagctggtc ccggtgctgc
     5941 tccgggacgt gcgcggtcgc gaggaagttc ggcgcggcgc cgttcgccca gctgccggtc
     6001 ccttcgagct ggagcgggcc ttcgaccgtg cgcagcgcgg cgtgcacggt ggggccgacc
     6061 gccttgaggt gcacttcata ttcacccagg ggcgagatgg gcgccagcgc cgagcccgcg
     6121 gcgcgccatt gcagcgtcgc ctcgccgtcc gcgcggccgc gctcgacgga caggcgcggg
     6181 atcttcacca gcacgtcccc ggtcaggcga aagggagcga gcttgggcat gcccaggccg
     6241 agcgccgcgg ccggcaggcg gatgccggcg tccgcaatct cgacctgcga cagcgaaatc
     6301 gtgaccggga acggcttggc ggtctggtcg aggtcgactt cggctacgaa gcgtccacgc
     6361 agcaacgatg cgggccgcac gcgccacgcg aggggctttg cgactccggc cctgcctgcg
     6421 gcatcgcgga tctcgagcca gccggtgccc gcccacaagc tgccctgcgc gctggcaagg
     6481 cgcagcctgc cgtcgctcgc acgtgccagc tgcgcatcga tcagcgtcgc cggcgcgagc
     6541 gcgagcaatg ccacggcaaa agcgcccagg ccgaacgcgg cggcgctcca gcgcctcacg
     6601 gcgccctggc gcgggtgagc gtcgccgtcg cgccgaccat gcccggcgcg gacagggctt
     6661 cgacgcgcgc cgactcgagg cgcacgttct gcgattgcag ggccgcgatc cagccgagcc
     6721 agtcggcgac ggacaccgcg cccaacgtga cgcgcacgtg atcggcgtcc ggcgcatcga
     6781 ggcgcgtcag cgcgcgggac aggccggcgg cgtccacgcg tgcctgtacc agcgcgcgca
     6841 gatcggtcgg cgaaactgcg ggcgcgggcg ttgcgcgcag gcgggcgagc tcggtcgcct
     6901 gctggtcgag gatcgccgcc tgcgtgcgca gggtatcgac gctcgcgctc agttgcctgc
     6961 ggccgcgctc ggccgagtgc accaccagca cataggacgc aacgcccagg actgccgcag
     7021 ccacggcgat gacgatgcgc tcgcgcggcg cgcgcgattc ccaggctctg cgcagcgcgg
     7081 ctttcatggc gcgcgcaccg tgatgagctt gccgccaagg ttcttcgtat cgaccgcggc
     7141 gacatcggcg atcaatcccg tctgggtcag gcgcgccacg gtgcggcgca gcgccggctc
     7201 atcgattgcg gcgagctcga gggtcatgcg cccgctctcg taggacacgg tgcgcaaccc
     7261 gcccgcgggc agctccttca gcccgagcgc cactttctcg atcatcggcg cgaagtcgcc
     7321 ggcgtcgggc acgccggctc gatggcgcgc cgcggcgagc tggcggcgca tctgcagcgc
     7381 cgggtccgcg accgcgacgg cgtcgggaaa ggcgctgcga aagcgtgcct ccatcccggc
     7441 gcgcaccgag cgctgctcgg cggcgagccg catccagtcg gtgaccagcg cggtaccgtg
     7501 gagggcaagt gccgcgccgg cgatccaggc tgccggccgc aggctcgcca gcgcgccagg
     7561 ggcgatgcgc cagcgtcgcc gctcctgcgc caggctgacg ccggcttgct ccggcgccgt
     7621 gcgccagtcc caggcgcccg cggcatacag gggaatcccc agctcgcgct gccaggccgc
     7681 caggtccggc gccgtgccgt aaaccgcgag ggaatcgggc cgcgagcgct cgagcagcag
     7741 gcgcagctcg agcggcggcg aggccgcatc gccgctgtcg gtcgcggtgc cctcgaattc
     7801 tccggtgcgc acgaaccctt cggcgccgtc ccacgcaagg ctccattcgc ggttcgcgcg
     7861 cggcaacagc aggatctccg agtgcacctc ggcggcgcgc aggcccgcgg cccgcagcgc
     7921 gtcgcgccag cgcttcagcg ccgccttgtc gagcgcggca aacgcgtccg cgccgagcca
     7981 gctcacctgg tttgcttcgg gctcggctgc cgtgacgtct tcgagcgcaa atgcgagcac
     8041 cgatccgccg ttccggcgcg ccgatggcgg cagctccgcg cgcgtgatca gcacctgcgc
     8101 cgcgggaatg accagctgga cgcgaccggc gccgcgcggc agctcggcga gcgggccctc
     8161 gcctcgaacg acctcgctct cgtggatgag agcccactcg cagcgcagcg gcgcatcgcc
     8221 caatggcgcg taaatgcgca gcaggctcat ggatattttc tccacacgac cgtgggccag
     8281 ccggtcgact cgcgttccag cagcgcgctg ccgcgcgcct gcgcgccgcc gatggtcacg
     8341 cgcgcgctcg ccacgaagta ctggctggcg gtcgtgacgt cgttgccgtc ggccacggcc
     8401 ccggagggta gctgcgcgag gaactcgggg agggtgcgga aataggcgcg gccgcgctgc
     8461 gcggtcaggg cgcgcgcctg gtcgagatcc aggttgctca cgacggcggc gagcacctcg
     8521 ggcgaggcgg tgttcacgtt caccggggtg aagcggggga gcgccgtgac gaacggacgc
     8581 aggcgcttgc gcgcggcttc gtcgaagccg cgcaccagcg cgagctcggc gacatcgccg
     8641 agcggccggt tggctgcgag gtacggcggc tgcagcccct ggtagtagtc gctttcagcg
     8701 ccgtcctgcg gctgcggctc gccgtcggcg tcgagccagt cggccacgct tgcggcgagc
     8761 gtgtcgggca aagccaggat ctccagcagg cgccggaact gcgcgacatg cgcagggctg
     8821 accttgccgc ttctcacgag gttgttcaga ttgaacaagc cctgctgctc ctcgatctgg
     8881 ccggcgagct cgccgttgtc gaccgggacc ggcggcaggc gcaacgccca gggctcgcca
     8941 ggatggtcga tgttgctcaa gcgtcgatcg tcgctcagca gcgcgcgtcc ccagtcaatg
     9001 ccggcctgca cgagcgcctg cgcctgcgtg tgctcggcgc cgagctctgc ctgccgcgac
     9061 caggtgctct gcgaggcgag aatcgccgcc gcggcaatcg cggcgaacgc gacgacgccc
     9121 aacgcgagca cgatcgccac gccgcgctgt cgcagggcgt tcatctgagc gagaacacgc
     9181 gcaccagctc ctcgcccgag gcgagcacca ggcgcagccg taccgcccgc ggcaacggcg
     9241 gatcgcgctg cgaacggggc catgcgccgg cccacgccag attgccgtcc agatactgca
     9301 gctcgaactg cgccaccgcg ggcagcacgg cgtagcgcgt cggctccgcg ttgggcgcga
     9361 cgtcgagacc cggccacagc gtgatctcga tctcgtggtt ctcgttcagc cggtaggcga
     9421 cgcggcgcgg cgcgtcgatg ccctcggacg tggcaaagcg ggtgaactcg aagcgcccgg
     9481 cggcagcgcg cacctcgcgc ggcgccgaca gctggacatc gcgctggaag cgcgaataga
     9541 acgccgccac gcgcttccac ttctcggtct cctgcctgac gtggtcgcgc gcatccagca
     9601 ccgtgcccag cccgcggaac gacatcagcg cgagcagcga caggatggcg agcgcgatca
     9661 tcagctcgat cagcgtgaac ccgcgcggtc tcatcggggt tgcgccacga aacccgtgag
     9721 gcgggcaagc gagcgggact cctgcgccgg ctgcgatacg aacacgtcga cgcggcgaaa
     9781 cgccgagttg ggcgtggcga tcacctcctc gcgccaggcg agctcgacgc cgccctggct
     9841 ctcggtgccg cgggcgatgc cgagcggcag ccagtcgccc cgcgcgcggt gctcggcgag
     9901 ccggttctcc gcgacccagc ccgcgagcat ccgcaggcgc agctcgctca cggcgctggt
     9961 tccctgcccc gcggcgcgca gcgcagccat cagcgcgatc gacacgatgg cgagcgccac
    10021 cagcacctcg accagcgtga agcctgccgc gaagctccat ctactgcggt tcatggatcc
    10081 gcacctcgcc gagcggagac gccgcgaccg agtagcgcgc cgcgccgagc gacatctgga
    10141 actcgtacgc gagcgagccc tccggagcga actcgaggcg catgctcccc ggcgggcgca
    10201 tggtttcgat ccgcaggtcc gagatcgcca ttccgggcgg caggctgcgc gcgcgcagcg
    10261 catcgtcctg cacctcgatc cagccggtat cttcgcgcca gcgccagaac tggtagcgcg
    10321 ccgcgtccgc tgtccatgcg accggtcttc ccgtgaggcg cgattccacc gtggcgagat
    10381 cgagcagctg cgccagccgc tccgcctcga gccgcagcag cgcgcgctca tcgggccggg
    10441 tgatcgtggc gaccaggccg gcaaacagcc cgatgatgag cagcaccacg agcagctcca
    10501 atagagtgaa gccgcacttg tgcggcgggg tgaagcccct agaggctcca ggagccgata
    10561 tcggcatcgc tgccctctcc gccgggcgcg ccgtcgccgc ccatgctgga aacatcgagc
    10621 tcgccgtgca cgccgggatt gaggtactgg tagggattgc cccaggggtc cttcggcagg
    10681 cgctcgacat agccgccggc cttccagttg ggcgcgggcg gcgccgacgc cggccgcgcg
    10741 accagcgcct gcagtccctg ctcggtcgtg gggtagcgct ggttgtcgag gcgatagagc
    10801 ttgagcgcct gcatcaggct cgcgatgtcc tgttttgcgg caatcgcgcg cgcctcgtcc
    10861 gggcggctga tgatcttggg caccacgaga gcggcgagga tggccagaat gacgacgacc
    10921 accatcactt cgagcaatgt aaagccacgt tgcatcggga acctcctgca ctcaattacg
    10981 ctgtaagccc cggatcgact gttcggcacc gtacatacgg cgcatcgcgc cggccgtagc
    11041 atcggctcat ttagcggggg cggtatacgt ttgaacaaag cggcatttct cgcaacattg
    11101 gccgcggcct cgctgctcgg cgtggtggct gcgtactgga gctgggcgtt cctcgcgccg
    11161 gctcccacgc cgcgcgcgcc acccttggca gacttcgcag gctcgagctc gtccgcacgc
    11221 gggatgttcg gcagcgtaaa ggacggggca gcgcccgcgg cctcaagcgc agtgcgcctc
    11281 accggcgtag tggccgcatc ggacggcagg cgcgggcacg cggtgctgcg gcttgacggg
    11341 aagagaacgg tcgcggtgct gcaaggcgaa gacgtcgagc cggggctgcg gctcgcggag
    11401 gtccacgcgg atcgcatcgt gctggaacga aacggcgcgc gcgagacgct cacctggccc
    11461 gagaagggcg gcaagtgagg ctggctgcgc tccttttggt tgtcttgatg gctccactgc
    11521 aggcgcaggc gcaggcgcag gcgcccgcgc ccgacactcc actgcaggcg caggcgcccg
    11581 acacggtaac gctcaacttc ctcaacgccg acatcgaggg cgtggtgaag gcggtgagcg
    11641 agatcaccgg caagaacttc gtgctcgacc cgcgcgtgaa gggcaccatc aacatcgtct
    11701 cggcgcggcc ggtgccgcgc gcgctggtgt acgacgtgtt cctctcggcg ctgcgcctgc
    11761 agggctacgc cgcggtcgag gaccgcggcg tggtcaagat cgtgccggag gccgaggcca
    11821 agctccaccc gggccgcggc caggtccaga cgcgggtctt cacgctgaag tacgagtccg
    11881 cggcgcagct cgtgccggtc ctgcggccgc tgatcccgcc cgcgaacacg atcgcggcct
    11941 atccggggag caacgccctg gtgatcaccg actacgccga caacctgcag cgcctcgcga
    12001 ggatcatcga ctcgatcgac cagccgggcg gtaccgaccc ggtggtgatc ccgctcgcac
    12061 acgcttcggc gctcgatgtc gcgcagatgg tgaaccgcgt gttcgccgag gcgccgcagg
    12121 cagcggcgcc cggagccgac acctcgcagc gcttgagcgt ggtggcggat gcgcgctcca
    12181 acagcctggt tgtgcgcgcg gacaacccgt cgcgcatcgc ccggctgcgc accctggtcg
    12241 cgatgctcga ctcgcccacc agcgccgcgg gcaacctgca cgtcgtctat ctgaagaacg
    12301 ccgaggcggt gaaggtggcc gagacgctgc gcgcgatcta tctgggggag accgttgctg
    12361 ccgcagctcc ccggcccacg accgcttctg cgatgacccc tgcgagtacc tccccggtgg
    12421 gcgccccgct cgcagccctg cctgcgccgc aggccgtcgc tctcgtcccc ggcatgatcc
    12481 aggccgatcc ggcgaccaac tcgatcctca tccacgcgcc cgacacgatc tacaagaacc
    12541 tgcgcgcggc gctggacaag ctcgacgtgc gcagagcgca ggtctacgtg gaggcgctga
    12601 tcgccgagat caccgccgac aaggccgcgg aattcggcgt ccagtggcag agcctgagcg
    12661 gcgtggaaaa gagcggcacg aacgcgttcg gcggcacgaa cttcggcgga cccggccaga
    12721 acgtcctggg cattgcggcc aaccccgcca ccgccggccg cggcctcaac gtcggcgtga
    12781 tcaacggcgt ggtgaacatt cccggcatcg gcgagatcct caacctcaac ctgctgatcc
    12841 gggcgctcga gagcgacagc aacgccaaca tcctgtccac cccgacgctg ctgaccctcg
    12901 acaacgagga agcgaggatc atcatcggcc agaacgtgcc gttcattacc ggctcgtacg
    12961 cggtgaccgg cgcagcgacc acgccgacgc cgttccagac cgtcgagcgc aaggacgtcg
    13021 gcctcacgct gcgggtgaag ccgcagatct ccgagggcgg cacggtgcgg ctgcagatct
    13081 accaggaagt ctcgagcgtc gtcgacacga tactgcccaa ccccgccggc gtcaccacca
    13141 acaagcgcgc cgtcgaatcg accgtgctgg tggacgacgg gcagatcgtg gtgatcggcg
    13201 ggctgatcca ggatggcgtc
//
LOCUS       DATBSZ010000028        15050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1915406, whole genome shotgun sequence.
ACCESSION   DATBSZ010000028 DATBSZ010000000
VERSION     DATBSZ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15050
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915406"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VIG70_01675"
     CDS             complement(<1..308)
                     /locus_tag="VIG70_01675"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY8249286.1"
                     /translation="MNLAIFGATGTIGANTLDVVARHAGRYRVFALTANASAEPLAEL
                     CRRYRPRYAVLSAVGEDRALKRRFSEFDTELLFGPEALEVVARHPETQTVMAAIVGAA
                     G"
     gene            complement(325..1110)
                     /locus_tag="VIG70_01680"
     CDS             complement(325..1110)
                     /locus_tag="VIG70_01680"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY8249287.1"
                     /translation="MPDPLRRAGTAAVLLVILLAALFLLPRAGIAALMALIVAIGGLE
                     WGRLCGLGVIAARLYAGALVLALLVLFVLGISSPLFLLGCVLWIVAVPLWLWLGVKPE
                     HRAALAAAGFLVLLPPALAMVVLSPGEALLVLTVAWIADTAAYFAGRRWGRRKLAPAI
                     SPGKTWEGAAGGLIAAAAYAMILSTFPAGAQATRMAAFLGAAALLVMVSIVGDLFESA
                     AKRQAGVKDSGALLPGHGGVLDRIDSATATLPVAAVLIPWLKA"
     gene            complement(1127..1882)
                     /gene="uppS"
                     /locus_tag="VIG70_01685"
     CDS             complement(1127..1882)
                     /gene="uppS"
                     /locus_tag="VIG70_01685"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY8249288.1"
                     /translation="MSSTREIPQASDTPRHVAIIMDGNGRWARRRRLPRIAGHRRGAE
                     AVRAALRACADRGVEYLTLFAFSSENWRRPPEEVALLMKLFRNALEREVDELHASGVR
                     LRVVGDTRRFDPKLRKLIERGEELTAQNRRLTLTIAANYGGRWDILQAMGRLLKDKPS
                     LEQIDEELLAPYLAMSYAPEPDLFIRTGGEQRISNFLLWQLAYSELYFTDTLWPDFDA
                     AALDAAIASFRGRERRFGRTSEQLEQSQRPEVA"
     gene            complement(1879..2439)
                     /gene="frr"
                     /locus_tag="VIG70_01690"
     CDS             complement(1879..2439)
                     /gene="frr"
                     /locus_tag="VIG70_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY8249289.1"
                     /translation="MVIAELKKTTDQKMDKSLQAFKADLGKVRTGRAHTGLLDHVTVD
                     YYGTPTPINQVAKIILLDARTIGVTPFEKKLIPTIEKGIRESDLGLNPVTSGDTIRLP
                     MPALTEERRKELVKVVKHEAENARVAVRNLRRDAIQQLKDALKKHEVSENDERRALDE
                     MQKMTDRHIADIDRLLQEKEKELMAL"
     gene            complement(2456..3169)
                     /gene="pyrH"
                     /locus_tag="VIG70_01695"
     CDS             complement(2456..3169)
                     /gene="pyrH"
                     /locus_tag="VIG70_01695"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY8249290.1"
                     /translation="MTPKYKRVLLKLSGEALMGDDPFGINRQTIESIVSEVAQVSRMG
                     VELGVVIGGGNIFRGLAPGAAGMDRATADYMGMLATVMNALALSDAMRQAGIASRVQS
                     ALNIEQVVEPYIRGKAIRYLEEGKVVIFAAGTGNPFFTTDTAAALRGSEMGAEIVIKA
                     TKVDGVYTADPKRDPRAERYAKISFDEAIARNLRVMDATALTLCRDQKLPIAVFSIFK
                     PGALARVVLGEDEGTLVHV"
     gene            complement(3166..3978)
                     /gene="tsf"
                     /locus_tag="VIG70_01700"
     CDS             complement(3166..3978)
                     /gene="tsf"
                     /locus_tag="VIG70_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY8249291.1"
                     /translation="MAEISAGLVKELRELTGLGMMECKKALTEAGGDLKKAEELLRIK
                     SGAKASKAASRIAAEGIIGSFLSADSKLGALVELNCETDFVARNDDFIAFAKSLAEIA
                     ATRDPGPAEEDRRQKLVQKIGENITIRRFKRMQGKGKLTVYVHAGSKIGVLVDYEGAD
                     EVGKDLAMHIAFAKPAYLTKAQIPQEMVQDERRIQEARAKESGKPPEIVAKMVEGAVN
                     KFLGEKTLGGQPFIKDDKQTVEKMLSAKKAKVHGYAFLVVGEGIDKPGTSVH"
     gene            complement(4047..4796)
                     /gene="rpsB"
                     /locus_tag="VIG70_01705"
     CDS             complement(4047..4796)
                     /gene="rpsB"
                     /locus_tag="VIG70_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY8249292.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRYWNPKMAPYIFGHRNKIHIINLEK
                     TLELYQAALKFARQQAANRGMILFVGTKRQAREIVREEALRCQMPFVDHRWLGGMLTN
                     FKTVKQSIKRLKEMEAMQQDGSLERISKKEALGLTRELAKLTRSLGGIKELTAPPDAL
                     FIIDVGYQKGAVAEANKLGIPVIGVVDTNHTPEGIQYVIPGNDDSSRAIRLYARGMAD
                     AVLEGRTQSLEEVVAASRDEFVEVDEDEAKQ"
     gene            4913..5701
                     /gene="map"
                     /locus_tag="VIG70_01710"
     CDS             4913..5701
                     /gene="map"
                     /locus_tag="VIG70_01710"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY8249293.1"
                     /translation="MAVVLKSSDELERMRIAGRLASEVLDFITPHVRPGVTTGKLNDL
                     CHEHMVAVQGTVPAPLNYAPPGYRPFPKSICTSVNHQVCHGVPGERVLKAGDILNIDI
                     TVIKDGYHGDCSRMFYVGEPSIQAKRLVEVTFECMWLGIRQVRPGGRLGDVGAAIQAH
                     AEKQGFSVVREFCGHGIGRKFHEEPQVLHYGKAGAGVALEPGMTFTIEPMINAGKPGI
                     RELADGWTIVTKDHSLSAQWEHTVAVTESGFEVLTQSNGTQAAP"
     gene            5698..8262
                     /locus_tag="VIG70_01715"
     CDS             5698..8262
                     /locus_tag="VIG70_01715"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY8249294.1"
                     /translation="MKFDLRAEVAAARQALKDAYLKRPNPRALLSKHSQLVDRTVKAL
                     WGETGVAADAALVATGGYGRGELFPCSDIDLLVLLGRDPSEAERDALERLIGRMWDIG
                     LEIGHSVRTVEGCIEAAAGDITIRTTLLESRFLVGSRPLFQKLQKQLIFEPVAFFKAK
                     KLEQEQRHAKHQDSPYSLEPNLKEAPGGLRDLQVIQWMARASGMGRRWSDLVKHGLIE
                     RGEARELGKLENHLHDLRIRLHYLAGRREDRLVFDLQAALAAQLGHRDTPSRRASEAM
                     MQRYYQTAKGVTQLNTILLQNLEARLAPRPDTGPRPLNERFEVRSELLHIRQESTFER
                     SPTAILECFLLMMQHPELRGMTARTLRALWRARFLVNAKFRKDATARLLFLHVLQQPR
                     GIVHEFRRMNQYGILGRYLPEFGRIVGQMQHDLFHVYTVDQHILMVMRNLRRFTMPEF
                     AHEFPLCSELMNGFERRWLLYVAALYHDIAKGRGGDHSELGAKDVRVFSRNHGLARED
                     RELVEFLVERHLAMSHVAQKQDVHDPEVIKAFGELVGTERRLVALYLFTVADVRGTSP
                     KVWNAWKGKLLEDLFRATRRALTGEPLARDAALAEKQAEAGRLLRLYALSDAVKDKLW
                     AGLDTAYFLRHDAQEIAWHTRNLHYRVDAATPVVKARLAPFGEGLQVMIYTRDREALF
                     ARICGYFERAGFNIAEAKVHTTRNGYALDTFVIMGEGRDAHYQDRIAMIETDLAAELQ
                     SDAPLGPPRGGRLSRRVRHFPISPAVNIRPDERGAYHALTIVAADRPGLLYGVARTLS
                     RYRINLQTARINTLGDRAEDVFLISGEVLKDSKAVLQLEQELLEDLTLREPEAALR"
     gene            complement(8259..9074)
                     /locus_tag="VIG70_01720"
     CDS             complement(8259..9074)
                     /locus_tag="VIG70_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEY8249295.1"
                     /translation="MVYKEPGEKVRLERISPDPPKGMTKERAQRRFAELGEELFELQD
                     AMFGAKVNSVMVVLQGRDSAGKDGAIKHVAGCLNPRGVNVVSFGVPTAEEREHDFLWR
                     VHRHAPRLGEFAIFNRSHYEDVLVVRVHGLVPRKLWQERYGHIVDFEELLAEHGTIVL
                     KYFLHVSRKEQKERLLERERTPQAAWKLNVNDWKEREHWDDYTEAYEDAISKTAAPHA
                     PWIVVPANAKWYRNLVVAESIAEALRGHRKSWRKALDETGRKRRAELADYRKG"
     gene            complement(9076..9609)
                     /gene="def"
                     /locus_tag="VIG70_01725"
     CDS             complement(9076..9609)
                     /gene="def"
                     /locus_tag="VIG70_01725"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY8249296.1"
                     /translation="MIRDVLRMGDPRLWQKSLPVSQFSSEELNELLRDMRDTMAHLNG
                     AGLAAPQIGVPLRLVIFGVTANPRYPDIEPVPDTVLINPVLTPLSNEMEEGWEGCLSV
                     PGMRGWVPRHRELKYAGCDEHGNPFERTVQGFHARVVQHEVDHLDGVLYPMRIRDLTK
                     FGFNEELFPGAGLPPED"
     gene            complement(9606..10343)
                     /locus_tag="VIG70_01730"
     CDS             complement(9606..10343)
                     /locus_tag="VIG70_01730"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HEY8249297.1"
                     /translation="MLAIIGGSGLTQLANLEVGRRKVARTPYGDPSGPVTFGRIGSNE
                     VLFLARHGYGHTIAPHEVNYRANLWALKQAGADSVVSVASVGGIRNDIWPGAIVLPHQ
                     VIDYTWGRASTYFEGPGTPVNHIDFTEPYSRELRARLLKAAAACGERVTDGGVYAATQ
                     GPRLESAAEINRLERDGAEIVGMTGMPEAALAREISLQYAAIAVVANYAAGRGDSERA
                     VPLDKIEAVLDEAMGRVRRIIEKLCEQ"
     gene            complement(10346..10891)
                     /locus_tag="VIG70_01735"
     CDS             complement(10346..10891)
                     /locus_tag="VIG70_01735"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEY8249298.1"
                     /translation="MPDTESAWAFLRDSDLIASAAEVQAAVQRVASEVEEKLARSYPL
                     VLAVMGGAVVFAGQILPLLRFPLDFDYVHASRYGAMTRGSRIEWRVRPPALVRGRAVL
                     VLDDILDHGETMNAIRNGLLELGAQTVHCAVLVEKTLKEAKPIKPDFVGLRIPDRFVF
                     GCGMDAKGFWRNLPEIRAMKE"
     gene            complement(10884..11759)
                     /gene="galU"
                     /locus_tag="VIG70_01740"
     CDS             complement(10884..11759)
                     /gene="galU"
                     /locus_tag="VIG70_01740"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY8249299.1"
                     /translation="MHAKLRKAVFPVAGHGSRFLPVTKASPKEMLPIVDKPLIQYAVE
                     EAAAAGITEMIFVTGRNKRAIEDHFDKAHELETELERKNKGVLLDMVRSVLPEGVSCI
                     YLRQAEPLGLGHAVLCAQPVVGNEPFAVLLADDLIDSQPPAMCRMAEVFASTGSSLLG
                     VEEVPREQTQSYGIVTIDKLQGDTARIHSIVEKPKPDKAPSNLGVIGRYVLTPRIFEL
                     LGQVVPGAGGEIQLTDAISALLVIERVLAVRLPGRRYDCGSKLGYLQATLEYGLRHPE
                     TGADFARYLKSRKDA"
     gene            complement(11766..13751)
                     /gene="ligA"
                     /locus_tag="VIG70_01745"
     CDS             complement(11766..13751)
                     /gene="ligA"
                     /locus_tag="VIG70_01745"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY8249300.1"
                     /translation="MTIEKKIRQLRETIERHNRRYYVEDAPEITDAEYDRLFRELQAL
                     EAEHPALRSADSPTQRVGAAPLSEFGEVRHGAPMLSIANAFDEEEVRAFDKRVREGLK
                     VEEVEYAAEPKFDGLAVSLVYRNGVLFQGATRGDGTTGEDVTANLRTVHSIPLKLNSS
                     DESLEVRGEVLMYRRDFEALNQRQRQAGQKEYVNPRNAAAGAIRQLDSRITASRRLRF
                     FAYALISGTGAGTHSQALDRLEKLGFPVCEERGVVQGVDGLLAYYAKIGRRREKLPYA
                     IDGVVYKVNRLDWQERIGFVSRAPRFALAHKYPAEEQTTQVLGIEVQVGRTGTLTPVA
                     RLKPVFVGGVTVTNATLHNEGELRRKDVRIGDTVVVRRAGDVIPEVVSVRTEARPSNA
                     TIFQMPAKCPVCGSAVVRNEDEAAHRCSGGLYCPAQRKQAVLHFASRRAMNIEGLGER
                     LVDQLVEKDIVHTPADLYKLGAKELEDLERMGEKSAANLVAELERSRETTLERLIYAL
                     GIRNVGETTARDLARHFGDLGPLENASEEELLRAPDVGPVVARSIGQFFDEPHNRQVI
                     RDLVTRGVRWPKVAPAPRLSTPAKTFVLTGTLSGMSREEARAAIEAKGHKVAGSVSKK
                     TDFVVAGEDAGANLERARSLGVTILEEKEFTKMLQNL"
     gene            complement(13748..14590)
                     /locus_tag="VIG70_01750"
     CDS             complement(13748..14590)
                     /locus_tag="VIG70_01750"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HEY8249301.1"
                     /translation="MSDLVLGLLVLGGCLFAGVLVYNRLQERSARRAAERAFASPHAD
                     SLLQQAPRREPVLKVETAPDERVDYVIELRGRAPLAAAVMLEAWRPIEQRFARRVLLA
                     AADGGAWRAGLQLVSRAGVVSDAELIEFRSGVETLGARLGAAVSSPEMRPALDAAREL
                     DRTCADSDIQVALHVVGPMLDLNSEALAGEQPFKVVRREDGITLVLDVALTPDLGASY
                     EAMARAGRRLADSHGARLVDDRGNALDDRSLAAIGAQLEAVRQTLAGRGIEPGSPLAL
                     RLFS"
     gene            complement(14600..>15050)
                     /locus_tag="VIG70_01755"
     CDS             complement(14600..>15050)
                     /locus_tag="VIG70_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY8249302.1"
                     /translation="NGHLAKLFPTLFGGGDAKLTMTGEEILDAGVQVMAHPPGKRNTS
                     IHLLSGGEKALTAIALVFSLFQLNPAPFCLLDEVDAPLDDSNTLRFCDLVKRMSAQTQ
                     FLFISHNKISMEMASQLVGVTMPESGVSRVVAVDIEEALRLREELAA"
BASE COUNT         2345 a         5149 c         4977 g         2579 t
ORIGIN      
        1 ccggccgcgc cgacgatcgc cgccatcacc gtttgcgttt ccggatgccg ggcgacgact
       61 tcaagcgctt ccggaccgaa cagcagttca gtgtcgaact cggagaaacg ccgtttgagc
      121 gcgcgatctt cccccactgc ggaaagcacc gcatagcgcg gacggtaacg ccggcacagc
      181 tcggccagcg gctcggccga tgcgttcgcc gtaagcgcaa acacccggta gcgtccggcg
      241 tggcgcgcca ccacgtcgag ggtgttggcg ccgatggtgc cggtggcgcc gaaaatggcg
      301 aggttcatgc gaagagagcg acgttcatgc tttcaaccac gggatcagca ccgcagcgac
      361 cggcaacgtg gcggtggcgc tgtcgatgcg gtcgagcacg ccgccgtgcc ccggcaggag
      421 cgcgccgctg tccttgaccc cggcttgccg cttggccgcg gattcgaaca ggtcgccgac
      481 gatgctgacc atcacgagca gcgctgccgc gccgagaaaa gccgccatcc gggtcgcctg
      541 ggctcctgcg ggaaatgtcg aaagaatcat agcgtaggcg gccgcggcga tgaggccgcc
      601 ggccgcgcct tcccaggtct tgccggggct gatcgcaggg gcgagcttgc gccgtcccca
      661 gcgccggccg gcgaagtaag ccgcggtgtc ggcaatccac gccaccgtga gcacgaggag
      721 cgcctcgccc ggagagagca ccaccatggc gagcgccggc ggcaggagca ccaggaagcc
      781 ggccgcggcg agcgctgcgc ggtgctcggg tttcacgccg agccacagcc acagcgggac
      841 cgcgacgatc cacagcacgc agccgagcag gaacagcgga ctcgagatgc cgagcacgaa
      901 gagcaccaag agtgccagca ccagcgcccc ggcataaagc cgcgccgcga tgacgcccag
      961 cccgcacagg cgcccccact cgaggccgcc gatcgcgacg atcagcgcca tgagcgccgc
     1021 gatacccgcg cgcggcagca ggaacagggc ggcgagcagg atgacgagca gcaccgccgc
     1081 ggtgccggct cgcctcagcg gatcgggcaa ccgggaggaa acttcgtcag gcaacttcgg
     1141 gacgctgcga ctgctcgagc tgctcgctgg tgcgcccgaa gcgccgctcg cggccgcgga
     1201 acgaggcgat cgccgcatcg agcgccgcgg cgtcgaaatc gggccacagc gtgtcggtga
     1261 agtagagctc gctgtacgcg agctgccaca gcaggaagtt gctgatgcgc tgctcgccgc
     1321 cggttcggat gaagagatcg ggctccggcg cgtagctcat cgcgaggtag ggcgcgagga
     1381 gctcctcgtc gatctgctcg agcgacggct tgtctttcag gagccggccc atggcctgca
     1441 ggatgtccca gcgcccgccg tagttggccg cgatcgtcag ggtcagccgc cggttctgcg
     1501 cggtcagctc ctcgccgcgc tcgatcagct tgcgcagctt ggggtcgaag cgccgcgtgt
     1561 cgccgacgac cctgaggcgc acgccgcttg cgtgcagctc gtccacctcg cgctcgagcg
     1621 cgttcctgaa gagcttcatc aggagcgcca cctcctccgg cggccggcgc cagttctcgg
     1681 agctgaaggc gaacagcgtc aggtactcga cgccgcggtc ggcgcaggcg cgcagcgcgg
     1741 cgcgcaccgc ctcggcgccg cggcggtgcc cggcgatgcg cggcaggcgc cggcgccgcg
     1801 cccagcggcc gttgccgtcc atgatgatcg cgacgtgacg cggggtatcg ctcgcctgcg
     1861 ggatctcgcg tgtcgagctc atagcgccat cagctctttt tctttttcct ggagcagccg
     1921 gtcgatgtcc gcgatgtggc ggtcggtcat cttctgcatc tcgtcgagcg cgcggcgctc
     1981 gtcgttctcg gacacctcgt gcttcttgag cgcgtccttg agctgctgga tcgcgtcgcg
     2041 ccgcaggttg cgcaccgcga cgcgggcgtt ctcggcttcg tgcttgacca ccttgaccag
     2101 ctccttgcgc cgctcctcgg tgagcgcggg catcggcagc cggatggtgt cgccgctggt
     2161 gaccgggttc aagcccaggt cggactcgcg gatgcccttt tcgatcgtcg ggatgagctt
     2221 cttctcgaaa ggcgtgacgc cgatggtgcg cgcatcgagc aggatgatct tggccacctg
     2281 gttgatcggg gtcggggtgc cgtaatagtc gaccgtcacg tggtcgagca gcccggtgtg
     2341 cgcgcgcccg gtgcgtacct tgcccaggtc ggccttgaac gcctgcaagc tcttgtccat
     2401 cttctggtcc gtcgtcttct tgagttcggc aatcaccatg gcgacctccc cgccatcata
     2461 cgtgaaccaa agtgccttcg tcctcgccca gcaccacccg ggcgagcgcg ccgggcttga
     2521 agatgctgaa caccgcgatc ggcagcttct ggtcgcggca cagggtgagc gcggtggcgt
     2581 ccatcacgcg caggttgcgc gcgatcgcct cgtcgaagct gatcttcgcg tagcgctcgg
     2641 cgcgcggatc gcgcttcggg tcggcggtgt agacgccgtc gaccttggtc gccttgatca
     2701 cgatctccgc gcccatctcg ctgccgcgca gcgccgccgc ggtatcggtg gtgaagaacg
     2761 ggttgccggt gccggcggcg aaaatcacca ctttgccctc ttccagatag cggatcgcct
     2821 tgccgcggat gtagggctcc accacctgct cgatgttgag cgcggactgc acgcggctcg
     2881 cgatcccggc ctggcgcatc gcgtccgaca gcgcgagcgc gttcatcacc gtggcgagca
     2941 ttcccatgta gtcggcggtg gcgcggtcca tgccggccgc gcccggggcg aggccgcgaa
     3001 agatgttgcc gccgccgatc accacgccga gctccacgcc catgcgcgag acctgggcca
     3061 cctcgctcac gatcgactcg atcgtctggc ggttgatgcc aaaaggatcg tcccccatca
     3121 gggcttcgcc cgacagcttg aggagcaccc tcttgtattt gggagtcagt ggacgctcgt
     3181 cccgggtttg tcgatgcctt cgccaaccac gaggaaagcg tagccgtgaa ccttcgcctt
     3241 cttcgcggac agcatcttct ccaccgtctg cttgtcgtcc ttgatgaacg gctgcccgcc
     3301 cagcgtcttc tcgcccagga acttgttgac cgcgccctcg accattttgg ccacgatctc
     3361 cggcggcttg cccgattcct tcgcgcgcgc ctcttggatt cttctctcgt cttgaaccat
     3421 ttcctgggga atctgggcct tggtcaggta cgcgggcttg gcaaacgcga tatgcatggc
     3481 caggtccttg ccgacttcgt cggcgccctc gtaatccacc agcacgccga tcttgcttcc
     3541 ggcgtgaacg tagaccgtca gcttgccctt cccctgcatg cgcttgaagc ggcggatggt
     3601 gatgttctcg ccgatcttct gcaccagctt ctggcgcctg tcttcttcgg cgggacccgg
     3661 atcgcgcgtg gcggcgatct cggccagcga cttggcgaat gcgatgaaat cgtcattcct
     3721 cgcgacgaag tcggtctcgc aattcagctc gaccagcgcg cccagcttgc tatcggcaga
     3781 aagaaacgat ccgatgatgc cctcggcggc aatccggctc gccgccttgc tcgccttggc
     3841 gccgctcttg atgcgcagca gctcctcggc cttcttcagg tcgccgcccg cttcggtgag
     3901 cgccttcttg cactccatca ttcccagccc ggtcagctcg cgcagctctt tcaccaggcc
     3961 tgcactaatt tccgccatta cttgcagctc cttcaggttg aaaaaaaggg gccagaagcc
     4021 ccttttgtcg tgaatacggg ctcagcctac tgcttggcct catcctcatc cacctcgacg
     4081 aactcgtcgc gcgaggcggc gacgacctcc tccagagact gggtgcggcc ttcgagcacc
     4141 gcgtcggcca tgccgcgcgc gtagagccgg atggcgcggc tcgagtcgtc gttgcccggg
     4201 atcacgtact ggatgccctc gggggtgtgg ttggtgtcga ccacgccgat caccgggatg
     4261 ccgagcttgt tcgcctcggc gacggcgccc ttctggtagc cgacgtcgat gatgaagagg
     4321 gcatcgggcg gcgccgtgag ctccttgatg ccgccgagcg agcgcgtgag cttcgccagc
     4381 tcgcgcgtga gtcccagcgc ctctttcttg gagatgcgct cgaggctgcc gtcctgctgc
     4441 atcgcctcca tctccttcag ccgcttgatc gactgcttga cggtcttgaa gttggtgagc
     4501 atgccgccga gccagcgatg gtcgacgaac ggcatctggc agcgcagcgc ctcctcgcgc
     4561 acgatctcgc gcgcctggcg cttggtgccg acaaaaagga tcattccccg gttggccgcc
     4621 tgctggcgcg cgaacttgag cgccgcctgg tagagctcga gcgtcttttc gaggttgatg
     4681 atgtggatct tgttgcgatg gccgaagata tacggcgcca tcttcggatt ccaataccgg
     4741 gtctgatgtc cgaaatggac cccggcttcc agcatttgac gcatcgttac ggacacttcg
     4801 cttctccttt agggttcggt ctgcgacgcg ctctgccccc gagttttcct cgagagaccc
     4861 ttgtcgaacg cgccggaaga tttgagcccg aaattctagc ataatcaggc taatggccgt
     4921 ggtcctgaag agctcggacg agttggagcg catgcgcatc gccggccggc tcgcctccga
     4981 agtcctcgat ttcattactc cgcacgttcg gccgggtgtc acgaccggaa agctgaacga
     5041 tttgtgccac gagcacatgg tcgcggtaca gggcacggtg cccgcgcccc tcaactacgc
     5101 gccgccgggc taccgcccgt tcccgaaatc gatctgcacc tcggtcaacc accaggtctg
     5161 ccacggcgtg cccggcgagc gggtcctgaa agcgggcgac atcctgaaca tcgacatcac
     5221 cgtcatcaag gacgggtacc acggcgactg cagccgcatg ttctacgtgg gtgagccgag
     5281 catccaggca aagcggctgg tcgaggtgac cttcgagtgc atgtggctcg gcatccgcca
     5341 ggtgcgcccc ggcggtcggc tcggcgacgt cggcgccgcg atccaggccc acgccgagaa
     5401 gcagggcttc tccgtggtgc gcgagttctg cggccacggc atcgggcgca agttccacga
     5461 ggagccgcag gtgctccact acggcaaggc cggcgccggc gttgcgctcg agccgggcat
     5521 gaccttcacc atcgagccga tgatcaacgc cggcaagccc ggaatccggg agctggccga
     5581 cggctggacc atcgtcacca aggaccacag cctgtcggcg cagtgggagc acacggtcgc
     5641 ggtcaccgag tccgggttcg aggtcctgac ccaatcgaac gggacccagg cggcgccgtg
     5701 aagttcgacc tgcgcgccga ggtcgccgcc gcccggcagg cactgaagga cgcgtacctg
     5761 aagcggccga acccgcgcgc gctgctcagc aagcatagcc agctcgtcga ccgcaccgtg
     5821 aaggcgctgt ggggagaaac cggggtcgcg gctgacgccg cgctggtcgc gaccggcggc
     5881 tatgggcgcg gcgagctttt cccctgctcc gacatcgacc tgctggtgct gctggggcgc
     5941 gacccgagcg aggccgagcg cgacgcgctc gagcgcctca tcggccgaat gtgggacatc
     6001 ggcctcgaga tcggccacag cgtgcgcacc gtcgagggat gcatcgaggc cgcggccggc
     6061 gacatcacga tccgcaccac gctgctggag tcgaggtttc tcgtcggcag ccggccgctt
     6121 tttcagaaac tgcaaaaaca acttattttc gagccggtgg ccttcttcaa ggcgaagaag
     6181 ctcgagcagg agcagcgcca cgccaagcac caggactcgc cttactcgct cgagcccaac
     6241 ctgaaggaag cgcccggcgg gttgcgcgac ttgcaggtca tccagtggat ggcaagagcg
     6301 agcgggatgg gccggcgctg gagcgatctc gtcaagcacg gcctcatcga gcgcggcgag
     6361 gcgcgcgagc tcggcaagct cgaaaaccac ctgcacgacc tgaggatccg cctgcactac
     6421 ctcgccggcc ggcgcgagga ccgcctggta ttcgacctgc aggctgcgct cgcggcgcag
     6481 ctcgggcacc gcgacacccc gtcgcgccgc gcgagcgaag cgatgatgca gcgctactac
     6541 cagaccgcca agggcgtcac gcagctcaac accatcctgc tgcagaacct cgaggcgcgt
     6601 ctcgcgccgc ggccggatac ggggcccagg ccgttgaacg agcgcttcga ggtgcgcagc
     6661 gagctgctgc acatcaggca ggaaagcacc ttcgagcgtt cgccgaccgc gatcctcgag
     6721 tgcttcctgc tgatgatgca gcacccggag ctgcgcggaa tgacggcgcg cactctgagg
     6781 gcgttgtggc gcgcgcgctt ccttgtgaat gcaaagttca gaaaggatgc gaccgcgagg
     6841 ctgctattcc tccatgtcct gcagcagccg cgcggcatcg tgcacgagtt ccgccgcatg
     6901 aaccagtacg gcatcctcgg ccgctacctg ccggagttcg gccgcatcgt cgggcagatg
     6961 cagcacgacc tgttccacgt ctacaccgtc gatcagcaca tcctgatggt gatgcgaaac
     7021 ctgcggcgtt tcaccatgcc ggagttcgcc cacgagttcc cgttgtgcag cgagctgatg
     7081 aacggcttcg agcggcgctg gctcctgtac gtcgcggcgc tctaccacga catcgccaag
     7141 ggccgcggcg gcgaccactc cgagctcggc gccaaggatg ttcgggtttt ttcacgaaat
     7201 cacgggctcg cccgcgagga tcgcgaattg gtggaattcc tggtcgaaag gcatctcgcg
     7261 atgtcgcacg tcgcgcagaa gcaggacgtc cacgacccgg aggtgatcaa ggccttcggc
     7321 gagctcgtcg gcaccgagcg gcgcctggtg gcgctctacc tctttaccgt cgccgacgtg
     7381 cgcggcacca gccccaaggt gtggaacgcg tggaagggaa agctgctcga ggatctgttc
     7441 cgcgcgaccc gccgggcgct gaccggcgag ccgctggcgc gcgatgcggc gctcgccgag
     7501 aagcaggcag aagccgggcg gctattgcgg ctctacgcgc tgtcggatgc ggtcaaggac
     7561 aagctgtggg cgggcctcga tacggcctac ttcctgcgcc acgacgcgca ggagatcgcc
     7621 tggcacacgc gcaacctcca ctaccgcgtg gacgccgcaa cccccgtggt caaggcgcgc
     7681 ctcgcgccgt tcggcgaggg cctgcaggtc atgatctaca cgcgcgaccg cgaggcgctc
     7741 ttcgcgcgca tctgcggcta cttcgagcgc gccggcttca acatcgccga ggccaaggtg
     7801 cacaccacgc gcaacggtta tgcgctcgac accttcgtca tcatgggaga ggggcgcgac
     7861 gcgcactacc aggaccgcat cgccatgatc gagaccgacc tggcggcgga gctccagtcc
     7921 gacgcccctc tcggcccgcc gcgcggcggg cgcctgtcgc gccgcgtgcg ccacttcccg
     7981 atctcgcccg cggtcaacat ccgtcccgac gagcgcggcg cctaccatgc gctgacgatc
     8041 gtagccgccg accggccggg cctgctctac ggcgtggccc gcaccctgtc gcgctatcgc
     8101 atcaacctgc agaccgctcg catcaacacg ctcggcgatc gcgccgaaga cgtattcctc
     8161 atctccggtg aagtcctcaa ggactccaag gccgtgctgc agctcgagca ggagctgctc
     8221 gaggatctca ctcttcggga acccgaagca gcgctgcgct agcctttgcg gtagtcggcg
     8281 agctcggcgc ggcgcttgcg gccggtctcg tcgagcgcct tcctccagga cttgcggtgc
     8341 ccgcgcagcg cttcggcgat cgactcggcg accaccaggt tgcggtacca cttggcgttc
     8401 gccggcacca cgatccacgg agcgtgcggg gccgcggtct tcgagatcgc gtcctcgtag
     8461 gcctcggtgt aatcgtccca gtgctcgcgt tccttccagt cgttcacgtt gagcttccag
     8521 gcggcctgcg gcgtgcgctc gcgctcgagg aggcgctcct tttgctcctt cctgctgacg
     8581 tggaggaaat acttgagcac gatcgtgccg tgctcggcga ggagctcctc gaagtccacg
     8641 atgtgcccgt agcgctcctg ccacagcttg cgcgggacga ggccgtgcac gcgcaccacc
     8701 agcacgtcct cgtaatggga gcggttgaag atcgcgaact cgcccagccg cggcgcgtgg
     8761 cggtgcaccc gccagaggaa gtcgtgctcg cgctcctcgg cggtcggcac gccgaaggaa
     8821 acgacattca cgccgcgcgg attgaggcag cctgcgacgt gcttgatcgc gccgtccttg
     8881 cccgcgctgt cgcgaccctg cagcaccacc atcacgctgt tgaccttggc gccgaacatg
     8941 gcgtcctgca gctcgaacag ctcctcgccg agctcggcga agcgccgttg cgccctctcc
     9001 ttcgtcatgc ccttcggcgg atcgggtgaa atacgctcga ggcggacctt ttcgccgggt
     9061 tccttgtaga ccaactcaat cctccggcgg caggccggcg cccgggaaca gctcttcgtt
     9121 gaaaccgaac ttggtcaggt cgcgaatgcg catcggatag aggacgccgt cgaggtggtc
     9181 gacctcgtgc tggaccacgc gcgcatggaa gccttgtact gtcctttcaa aggggttgcc
     9241 gtgctcgtcg cagccggcgt acttcaattc ccggtggcgg ggaacccagc cgcgcatccc
     9301 gggcaccgac aggcagccct cccagccctc ctccatctcg ttcgagaggg gcgtcagcac
     9361 ggggttgatg agcacggtgt cgggcacggg ctcgatgtcg gggtatcggg ggttggcggt
     9421 gacgccgaag atcaccaggc gcaggggcac gccgatctgc ggcgcggcga ggccggcgcc
     9481 attcaggtga gccatcgtgt ctcgcatgtc ccgaagcagt tcattcaatt cttctgaact
     9541 gaactgcgaa acgggcaggg acttctgcca gagccggggg tcgcccatgc gcagaacgtc
     9601 gcggatcatt gctcgcagag cttctcgatg atgcggcgta cgcgccccat ggcctcgtcg
     9661 agcaccgcct cgatcttgtc gagcggcacg gcgcgctcgc tgtcgccgcg tccggcggca
     9721 tagttcgcga ccaccgcgat ggctgcgtac tgcaagctga tctcgcgcgc gagcgcggct
     9781 tcgggcatgc cggtcatgcc gacgatctcg gcgccgtcgc gctccaggcg gttgatctcc
     9841 gccgcgctct cgagccgcgg gccctgggtg gcggcgtaga cgccgccgtc ggtgacccgc
     9901 tcgccgcacg cggcggcggc cttgagcagc cgcgcgcgca gctcgcgcga gtacggctcg
     9961 gtgaaatcga tgtggttgac cggcgtgccc gggccttcga agtaggtcga tgccctgccc
    10021 caggtgtaat cgatgacctg gtgcggcagc acgatcgctc cgggccagat gtcgttgcgg
    10081 atgccgccga ccgaggccac cgacaccacc gaatcggccc ccgcctgctt cagcgcccag
    10141 agattcgcgc ggtagttcac ctcgtgcggt gcgatggtgt ggccatagcc gtggcgggcg
    10201 aggaacagca cctcgttgct tccgatgcgg ccgaaggtca cggggcccga gggatcgccg
    10261 tacggcgtgc gcgcgacctt gcgccggccg acctcgaggt tggcgagctg ggtgagtccg
    10321 cttccgccga tgatggccag catggctatt ctttcatcgc gcggatctcg gggagattgc
    10381 gccagaagcc tttcgcgtcc atgccgcagc cgaagacgaa acggtcggga attctaaggc
    10441 cgacgaaatc gggcttgatc ggcttcgctt ccttgagcgt cttctcgacg aggacagcgc
    10501 aatgcacagt ctgcgcgccg agctcgagca gcccgttgcg gatggcgttc atggtctcgc
    10561 cgtggtccag gatgtcgtcc agcaccagca ccgcacgtcc ccgcaccagc gccggcggcc
    10621 gcacgcgcca ttcgatcctc gagccgcggg tcattgcgcc gtagcgggag gcgtggacgt
    10681 agtcgaagtc cagcggaaag cgcagcagcg gcaggatctg cccggcgaac accactgcgc
    10741 ctcccatgac tgccaacacc agcggatagc tcctggcaag tttttcttca acttcggacg
    10801 cgacgcgctg caccgccgcc tgcacttccg cggcgctggc gatcaggtcg gagtccctga
    10861 ggaacgccca ggcgctctcc gtgtcaggca tctttccgcg acttcaggta gcgcgcgaaa
    10921 tcggccccgg tttccggatg gcgcagcccg tattccagcg tcgcctgcag gtagcccaac
    10981 ttcgagccgc agtcgtagcg ccgtcccggc aagcgcacgg cgaggacgcg ctcgatcacg
    11041 agcagcgcgg agatggcgtc ggtgagctgg atctcgccgc cggcgcccgg cacgacctgg
    11101 ccgagcagct cgaaaatcct gggcgtcagc acgtagcgcc cgatcacgcc gaggttcgag
    11161 ggcgccttgt ccggcttggg tttctcgacg atgctgtgga tgcgcgcggt atcgccctgc
    11221 agcttgtcga tggtgacgat gccgtagctc tgcgtctgct cgcgcggcac ctcctcgacc
    11281 ccgaggagcg aggatccggt gctggcgaac acctcggcca tgcggcacat cgcgggcggc
    11341 tgcgagtcga tcaggtcgtc ggcgagcagc accgcgaaag gctcatttcc aactaccggc
    11401 tgcgcgcaaa gcaccgcgtg gccgaggccg agcggctcgg cctggcgcag gtagatgcaa
    11461 cttacaccct cgggcagcac cgagcgcacc atgtcgagca gcacgccctt gttcttgcgc
    11521 tcgagctcgg tctcgagttc gtgagccttg tcgaaatggt cctctatcgc gcgcttgttg
    11581 cggccggtga cgaagatcat ctcggtgatg ccggccgccg cggcctcctc caccgcgtac
    11641 tggatcagcg gcttgtcgac gatcggcagc atttccttcg ggctcgcctt ggtgaccggc
    11701 aggaagcggc tgccatggcc ggcgaccggg aacacggctt ttctgagctt ggcgtgcatc
    11761 tggagttaga gattctgcag cattttggtg aattcctttt cttccaggat ggtcacgccg
    11821 agagagcgtg cgcgctcgag gttcgctccc gcatcctcgc cggcgaccac gaaatcggtc
    11881 ttcttggaca ccgagccggc cactttgtgc cccttggcct cgatcgccgc gcgcgcctcc
    11941 tcgcggctca tgcccgagag cgttccggtc agcacgaacg tcttcgccgg ggtcgacagc
    12001 cggggcgccg gggcgacctt cggccagcgc acccctctcg tgaccaggtc gcggatgacc
    12061 tggcggttgt gcggctcgtc gaagaactgc ccgatcgagc gcgccaccac gggaccgacg
    12121 tcgggcgccc ggagcaactc ctcctcgctt gcgttttcga gcggcccgag gtcgccgaag
    12181 tggcgcgcaa ggtcgcgcgc cgtggtttcg ccgacgttgc ggatgcccag ggcgtagatc
    12241 aggcgctcga gggtggtctc gcgcgagcgc tcgagctcgg cgaccaggtt ggcggcagac
    12301 ttctctccca tgcgttccag gtcttcgagc tccttggcgc cgagcttgta gagatcggcc
    12361 ggcgtgtgga cgatatcctt ttccacgagc tggtcgacca ggcgctcgcc gaggccctcg
    12421 atgttcattg cacgccggct ggcgaagtga agcaccgcct gcttgcgctg cgccgggcag
    12481 tacaggccgc cgctgcagcg gtgggcggct tcgtcctcgt ttctcacgac cgcggagccg
    12541 cacaccgggc acttggccgg catctgaaag attgtcgcgt tggacggacg agcttcggtc
    12601 ctcaccgaaa ccacttcggg aattacgtcg ccggcgcggc gcaccacgac cgtgtcgccg
    12661 atccgcacgt ccttgcgccg cagctcacct tcgttgtgca gggtggcgtt ggtcaccgtg
    12721 acgcccccca cgaacaccgg cttcagccgc gccaccggag tcagggtacc ggtgcgcccg
    12781 acctggactt cgatgcccag cacctgcgtg gtctgctcct cggcgggata cttgtgcgcg
    12841 agcgcgaaac gcggcgcgcg cgagacgaag ccgatgcgct cctgccagtc gaggcggttg
    12901 accttgtaga ccacgccgtc gatggcatag ggcagtttct cgcgccggcg gccgattttc
    12961 gcgtagtacg cgagcaggcc atcgacgcct tgcaccacgc cgcgctcttc gcaaaccgga
    13021 aagccgagct tctccagccg gtcgagcgcc tgtgaatgcg tccctgcccc cgtgccggaa
    13081 atcagggcgt aagcgaagaa ccgcagccgc cgcgacgcgg tgatccgcga atcgagctgt
    13141 cggatcgcgc cggccgcggc gttgcgagga ttgacgtact ccttctggcc ggcctggcgc
    13201 tggcgttggt tgagcgcttc gaagtcgcgg cgatacatca gcacttcgcc tcgcacttcc
    13261 agagattcat cagaggaatt caacttcaga ggaatcgagt gcacggtgcg caggttcgcg
    13321 gtcacgtcct cgccggtggt cccgtcgccg cgcgtcgccc cctggaagag gacgccgttg
    13381 cggtagacga ggctcacggc caggccgtcg aacttgggct cggcggcgta ttcgacctcc
    13441 tcgaccttga ggccttcccg cacccgcttg tcgaaggctc gcacctcctc ctcgtcgaag
    13501 gcgttggcga tcgagagcat cggtgcgcca tgccgcacct cgccgaactc ggagagcggc
    13561 gccgcgccca cgcgctgggt cggcgagtcg gcgctgcgca gcgccgggtg ctcggcttcg
    13621 agcgcctgca gctcgcggaa gagccggtcg tattccgcgt cggtgatttc cggcgcgtcc
    13681 tcgacgtagt agcggcggtt gtggcgctcg atcgtctccc ggagttgtct gatcttcttc
    13741 tcgatcgtca tgaaaagagc cgcagcgcga gcggactgcc gggctcgatg ccccgcccgg
    13801 cgagcgtctg gcgcaccgcc tcgagctgcg cgccgatggc ggcgagcgag cgatcgtcga
    13861 gcgcgtttcc gcgatcgtcg accagccggg cgccgtgcga atcggccagg cggcggccgg
    13921 cgcgcgccat cgcttcgtag ctcgcgccga ggtcgggcgt gagggcgacg tccagcacca
    13981 gcgtgatccc gtcctcgcgc cgcaccacct tgaagggctg ctcgccggcc agggcttctg
    14041 aattcaaatc aagcatggga cccacgacat gcaatgcgac ttgaatatcg ctgtcggcgc
    14101 aggtgcggtc gagctcgcgc gcggcgtcga gggccggccg catctcgggc gaggacaccg
    14161 ccgcccccag ccttgcgccg agcgtctcga ctccggagcg gaattcgatc agctcggcgt
    14221 cgctcaccac gccggcgcgg ctcacgagct gcaggccggc gcgccacgcg ccgccgtcgg
    14281 ccgcggcgag cagcacccgg cgcgcgaagc gctgctcgat cggccgccag gcttcgagca
    14341 tcacggcagc ggccagcggc gctcggcccc tgagctcgat gacgtaatcg acgcgctcgt
    14401 ccggagccgt ttcgaccttg agaaccggct cgcgccgcgg cgcctgttgc agcagcgagt
    14461 ccgcgtgggg cgacgcgaaa gcgcgctcgg cggcgcgccg cgccgagcgc tcctgcaggc
    14521 ggttatagac gagcacgccc gcgaacaggc accccccgag cacgagcaga ccgagtacga
    14581 ggtcgctcat cacgccgccc taggctgcga gctcctcccg caggcgcagc gcctcctcga
    14641 tgtccaccgc gaccacgcgc gagacgcccg actcgggcat ggtgacgccg acgagctgcg
    14701 acgccatctc catcgagatc ttgttgtggc tgatgaacag gaactgggtc tgcgccgaca
    14761 tgcgcttcac caggtcgcag aagcgcagcg tgttggagtc gtcgagcggc gcgtcgacct
    14821 cgtcgagcag gcagaacggc gccgggttga gctggaacag cgaaaacacc agcgcaatcg
    14881 cggtgagcgc cttttcgccg cccgacagca ggtggatgct ggtgttgcgc ttgcccgggg
    14941 gatgcgccat cacctgcacg ccggcgtcga ggatctcctc cccggtcatc gtcagcttgg
    15001 cgtcgccgcc gccgaagagg gtcgggaaca gcttggcgag atggccgttc
//
LOCUS       DATBSZ010000029        12995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_481053, whole genome shotgun sequence.
ACCESSION   DATBSZ010000029 DATBSZ010000000
VERSION     DATBSZ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12995
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481053"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4183
                     /locus_tag="VIG70_01760"
     CDS             <1..4183
                     /locus_tag="VIG70_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8249303.1"
                     /translation="LLRMVPDVTDVAQIDARGREQLSVSRLKMDELEKYPDRSQTPAF
                     KNARAGQTWFGPVYFRKETEPYMSIAVRSGDSGPVTVAEVNLKFMWDVITRIRIGQKG
                     KAYVVDGTGYLIADPDIGLVLRKTDLSGLEQVKAAFAPGADDSLAMLAKDPAGKEVLT
                     AYAPIEPAREKAGQARTGALGWKVFVEQPVSEVYSALDATILRTVALMIAGLVFSALA
                     AMWLARSMVRPINILQEGAQRIGAGDLDQKIDVRTGDELEALAGQFNRMTDQLRESYS
                     GLERKVEERTAELQETLEQQTATAEILKVISSSPTDVQPVFDAIVQSAARLFHPWNTS
                     ILMREGDLLDRKAFAGPQAALVDREELARIYPLPLDPKRSLAARAMLEGRVVEILDSQ
                     APDSPEAVRALGRAAGFRSVIAVPLMREGEGIGCIGLNHPEVGFRLRDKQLALVRTFA
                     DQAVIAIENVRLFNETKESLERQTATADILRVISSSTTDTQPVFDAIVKNAAALCNAM
                     FANAFSYDGEKLHFLATTTTSAPHLQVLRESYPMRPDRSQISGRTILARSVVRMEDGL
                     NDHEYSSRHAFAGSYRRMLGVPLMREGNPVGAIVVGWEDPGPIPAVQEQLLKTFADQA
                     VIAVENVRLFNETKESLEQQTATSEILKVISRSPTDTKPVFEAIVQSGLKLFPDALVS
                     VALPEDGQVLLAAVASRDAAYSDEWRKRFPFPLTREYMHGVAILDRRMVDVPDCAANT
                     DPVLAVGTRNFLASGLRAQTTMPMLRGDAAIGAISVVRREAGPLSENQIALLRTFADQ
                     AVIAIENVRLFNETNEGLERQTATSEILRIISDSPGDVKPILDAVAERATRLCDTAST
                     AIYVLEGDAMRRTAFNGPAELMGNETVAYTAGTLVGRAIVESKAIHVHDIERAQAEYP
                     VSWEFARKFGRHQTMLAVPLLREGRPFGTMLMRRWEVRPFTDKQIALSKVFADQAAIG
                     IENVRLFNETKEALEQQKASADVLGAISSSIADTAPVFDKILESCQRLFEGNLVGVTL
                     VEHDGQVHLSAYKGEKSEELARIYPMPLSRESGTGCAILDQKVVQFADLGQDSATPPQ
                     VIKGAQVLGFRSIIFGPMMFEGRGIGAIWVARRFAGPFSDKQISLLKTFADQAVIAIQ
                     NARLFREIQEKSAQLEVANKHKSEFLANMSHELRTPLNAIIGFSEVLSERMFGEINEK
                     QADYLKDIHESGRHLLSLINDILDLSKIEAGRMDLEISTFDLPTALSNAMTLVRERAQ
                     RHGIALSLEVDKRLGAFQADERKFKQIVLNLLSNAVKFTPDGGSVDVSARKHNGKIEV
                     AVRDTGIGIAPQDHAAVFEEFKQVGRDYTKKAEGTGLGLTLTKRFVELHGGAISLESA
                     LGKGSTFTITLPVRQ"
     gene            4194..4568
                     /locus_tag="VIG70_01765"
     CDS             4194..4568
                     /locus_tag="VIG70_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8249304.1"
                     /translation="MSTVLIIEDNEKNMKLARDVLQAKGYQTLEAVTGEDGVKLAKER
                     KPDLVLMDIQLPGINGIEAFKQIRGNAATGKIPVIALTASVTPTDRSAINAAGFDAFL
                     GKPINLKEFLDTVKRLVEKSKK"
     gene            4565..5590
                     /locus_tag="VIG70_01770"
     CDS             4565..5590
                     /locus_tag="VIG70_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8249305.1"
                     /translation="MTPAKILVVDDTPANVKLLSDLLSVKGYRVCTAVNGEEALAQVT
                     KEKPDLVLLDVMMPGISGYEVCKKIRENPETALLPVVMCTSLDPNQERVKGIEAGADD
                     FLSKPVNQPELFARVRSLLRIKGLQDERLARLKSFFSPQLAEAIAAGKGEDLLKTHRR
                     EITVEFFDLRGFTHFTETAEPEEVMDLLRAYHNALGKLVLEHQGTLERFAGDAVMVFF
                     NDPVPIDRPAEHAVRTALAMHEAFVPIGKHWEKRGYELALGVGIAQGYATLGAIGFEG
                     RWDYAAIGNVTNLAARLCAEAKAGQVLLDRKTMAALDGLFDFDPVGPLTLKGFSQPVP
                     AFALKKP"
     gene            5592..9053
                     /locus_tag="VIG70_01775"
     CDS             5592..9053
                     /locus_tag="VIG70_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8249306.1"
                     /translation="MGKPRRSTRTGKKAAPRRIERSRRAHPAAAKLAVALREYKEVLE
                     QQAATADILRIVAASVENADPVFAAITRAGQHLVSGSRVGLLVVRDGQLHQAAYSGIP
                     DEQVAILAKHFPAPLDRQTIAGASILDRRVIQVPDIAEVRERFPRSFRSARSSGDRAL
                     LVVPLLHGESAVGSLSIRRTKPGAFTARQVELARTFADQAVIAIRNARMFNETKEALE
                     QQTATAEILKVIAGSPTEVQPVFDVIVERAVRLCGARFGRVYRYDGSTIHMVASHGLS
                     AKGLGQVQRVFPRPAADDTIVGRVILARQPYFIEDVERDQSVPSLSRQMIEALGTRRQ
                     LTIPMLRAGEPIGAITMGWDKPAGFDDQEASLLKTFADQAVIAVENVRLFNETKDALE
                     RQTATAEILKVIASSPSDVQPVFNAIAASAKQLLGGLSGVVTRVDGGMQHLAAFTTTG
                     ESGDETLRRLFPMPRGELISGRAITTLAPAIRTDTETDPSISEAARENARARGYRSAL
                     AVPMVREGVAIGAISVARGIPGSFSDHQIGLLQTFADQAIIAIENVRLFNETKEALDR
                     QTAISEILEKISSSPTDVLPVLQAVAARAAHLCEGEQATVLMVEGDMLRPRVTYSSDQ
                     GPLPNPSTLVYLKRGYITGRAALDAEAINVEDAAALMDSEYTDGRENQQKLGYRSFLA
                     VPMMREGKAIGVIAVWRRFVRKFSDKHVALVKTFADQAAIAIENVRLFNELQARTEAL
                     TKSVRQLTALGEVGQAISSTLDLETVLKTIVSRAVQLTGLDAGAVYEYDERAEEFHLR
                     AAENMSEEIIELLRSTPIKKGDGAVGATAVAREPIQIPEIRDEGFKSQFREVLIRTGR
                     RALLAVPLMREDHIIGALAVQRNTPGPFAPEVVEVLKTFATHSAMAMQNARLFREIAE
                     KGRQLEVASEHKSQFLASMSHELRTPLNALLGFNEMILGDIYGEVPGDMRPPLAQMQT
                     SGKHLLRLINNVLDLAKIEAGRMELALADYSVQDVVEGVRSTLQPLAVEKGLEFLAAV
                     PADVPLAHGDSGRITQCLMNLAGNSLKFTKAGKVEISVVMNSGQLRYCVADTGIGIPR
                     DKIGSLFTEFRQTDATVASEYGGTGLGLSISKKFVEMHGGRIWVESEPGRGSVFTFEV
                     PLRAAAA"
     gene            9050..9424
                     /locus_tag="VIG70_01780"
     CDS             9050..9424
                     /locus_tag="VIG70_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8249307.1"
                     /translation="MSAATILYVEDNEANRMIVRDLLKRTKYTLIEAVDGEAGVAKAL
                     EARPGLILMDIQLPKISGLEAIRRLRAEPATAATPIIAITSFALSGDEQKAKAVGATA
                     YLAKPYSPFDLLKLVRQYLPES"
     gene            9421..>12995
                     /locus_tag="VIG70_01785"
     CDS             9421..>12995
                     /locus_tag="VIG70_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8249308.1"
                     /translation="MKRRKEPSAGVVRDGRREAAEAIKREAATAAILKAIRRSPGDAQ
                     PAFDAIVRSALRLVNGHSALVLRLADGMLHLAAHTSTGRSGDEAIRRFYPRPVAEVTL
                     HGRAIRSRRPCCVADTEKVSASMAAMKEMARKRGYRSLMAVPMLRDAVPIGSIGVSRK
                     QAGAFSRHQIRALETFADQAVIAIENARLFNETKEALERQTATAEILQVIAASPSDTQ
                     PVFDAIARSAQKLLGAHYANVTRVVEGALHLAAHTGTNPAGVESLKKVFPATLAGEGA
                     LGRAALSGEPACIADIETDPAYSASFREGARVRGYRSLLAVPMLRDGVSIGTIAVTRR
                     DPGPFSDHQIGLLKTFADQAVIAIENVRLFNETKEALEQQTATSEILKVISSSPTATQ
                     PVFESIVKNCHSLFGDSRVAVWQISDGRLVPRASTGYFPEPMPVDGESGIGACVLESR
                     TIHLPDLRSGAERYPRIKQLGLKHGYLSGVYAPLLREGQAIGGISLLRRQPGAFSDKE
                     VALLGTFADQAVIAIENVRLFKETKEALEQQKAAADVLRVISSSPTDIMPVYRTILEN
                     VTRLCEANIAALFLYDGEFIRTAAHRNATPEFAQQLDALRVPPSRETPTRRAALERKI
                     VHVEDVLSDPSFSLSEAHRREQPRTVLSAPLLREGGLVGVITVWRREPRLFTDKQVAL
                     VKTFADQAVIAIENTRLFQELQAKNDDLREALEQQTATGDILKVISRSTSDVKPVFET
                     IIRSAVRLCEGAFGTVFTFDGQVLDIGAEYNYSADVTEMFRREYPQPADRSTPSGRAI
                     VEGRVNNIPDIAESDYSQAVRDRAQANGWRGLLTVPMTRAGVAIGAIAVARREAGVFD
                     DSYVALLQTFADQAVIAIENARLFNETKESLEQQTATAKILGVMSSSPTDVQPVLDAV
                     AESAARLCETDDAIIRRVDSDGLRRVAHFGPLPATPETEIHPLVAGSVGGRAVLERRA
                     IHVEDIEEEVRQGRYPEASSLAAAAGWYRSMLAMPLIREDTAIGVIMIRRMEYRPFTD
                     KQIALLRTFADQAVIAIENVRLFNETKEALEQQTAISEVLRVISSSPADVKPVLDAVA
                     ERAARICSASDARIFLVEGEHLRHVAGFGDVGAPEVGVQRLIEPGIVMGRAVIDGRPM
                     HVEDIQAVPHEFPTGAQYGTQFGWRTILVVPLMREQRALGVISLR"
BASE COUNT         2353 a         4262 c         4396 g         1984 t
ORIGIN      
        1 gctcctgcgc atggtgccgg acgtcaccga cgtcgcgcag atcgacgcgc gggggcgcga
       61 gcagctctcg gtgtcgcgcc tgaagatgga cgagctcgag aaatatcccg accgctcgca
      121 gacgccggcg ttcaagaacg ctcgcgccgg ccagacgtgg ttcggaccgg tctacttccg
      181 caaggaaacc gagccctaca tgtcgatcgc ggtgcgctcg ggcgattccg ggccggtgac
      241 cgtcgccgag gtgaacctca agttcatgtg ggacgtcatc acccgcatcc ggatcgggca
      301 gaagggcaag gcctacgtgg tcgacggcac gggctacctc atcgccgacc ccgacatcgg
      361 cctggtgctg cgcaagaccg acctgtcggg actcgagcag gtgaaggcgg ccttcgcgcc
      421 gggcgccgac gattccctcg ccatgctggc aaaagacccc gccggaaaag aggtgctcac
      481 cgcctacgcc ccgatcgagc cagcccggga aaaggccggt caggcgagaa caggcgccct
      541 gggctggaag gtcttcgtcg agcagcccgt gtccgaggtc tactccgcgc tcgacgccac
      601 gatcctgcgc accgtcgcgc tcatgatcgc cggcctcgtg ttctcggcgc tcgccgcgat
      661 gtggctcgcg cgcagcatgg tgcggccgat caacatcctg caggaaggcg cgcagcgcat
      721 cggcgccggc gacctcgacc agaagatcga cgtgcgcacc ggcgacgagc tcgaagcgct
      781 cgccggacag ttcaaccgca tgaccgacca gctgcgcgag tcctactcgg gcctcgagcg
      841 caaggtcgag gagcgtactg cagagctgca ggaaacgctc gagcagcaga ctgcgaccgc
      901 cgagattctg aaggtcatca gcagctctcc aacggacgtg cagcccgtgt tcgacgccat
      961 cgtgcagagc gctgcgcggc tgttccatcc ctggaatacg tcgatcctca tgcgggaagg
     1021 cgatctgctg gaccggaaag cgttcgccgg accccaggcc gcgctagtgg atcgggagga
     1081 gctcgcaagg atctacccgc tgccgctgga tccgaagcgc agcctggctg cgcgtgcgat
     1141 gctcgaaggc cgggtcgtgg aaattctgga cagccaggca cccgatagcc cggaggcggt
     1201 aagagcgctg gggcgggcag ccggctttcg atcggtcatt gcggtgcccc taatgcgcga
     1261 gggggaaggg atcggctgca tcggcctcaa ccatccagag gtcgggttca ggctcaggga
     1321 caagcagctt gcgctggtgc gcactttcgc cgaccaggca gtcatcgcga tcgagaacgt
     1381 gcgcctgttc aacgagacca aggaatcgct cgagcgccag accgcgaccg ccgacatcct
     1441 gcgcgtcatc agcagctcga ccaccgacac gcagccggtg ttcgacgcga tcgtgaagaa
     1501 cgcggctgcg ctttgcaacg ccatgttcgc caatgccttc tcctatgacg gcgagaagct
     1561 gcattttctg gcgacgacca cgacgagcgc accgcacctc caggtgctgc gggagtccta
     1621 tcccatgcgc ccggatcgtt cgcagatctc gggccggacc atcctcgcgc ggtcggtggt
     1681 gcgcatggaa gacggcctga acgaccacga gtattcctcg cgtcatgcct tcgccggctc
     1741 gtaccgccgg atgctcggcg tgccgctcat gcgcgaagga aacccggtcg gcgcaatcgt
     1801 ggtgggctgg gaggacccgg gtccgattcc cgcggtgcag gagcaactgc tcaagacgtt
     1861 tgccgaccag gccgtgatcg cggtcgagaa cgtgcggctg ttcaacgaga ccaaggaatc
     1921 gctcgagcag cagaccgcga cctcggagat tctcaaggtc atcagcagat cccccaccga
     1981 taccaagccg gtgttcgagg cgatcgtgca aagcgggctg aagctgtttc cggacgcctt
     2041 ggtgtcggtc gcgctgcccg aagacggcca ggtgctgctt gccgcggtgg caagccgcga
     2101 cgcggcctac agcgacgagt ggcgcaagcg ctttccgttc ccgctgacgc gcgagtacat
     2161 gcacggcgtg gcgatcctcg accgccgcat ggtggatgtg ccggactgcg cggcgaatac
     2221 cgatccggtg cttgccgtgg gcaccaggaa cttcctggcg agcggtctgc gcgcgcaaac
     2281 caccatgccc atgctgcgcg gcgacgccgc catcggcgcg atcagcgtgg tgcggcgcga
     2341 ggcgggcccg ttgtccgaga accagatcgc gctgctgcgc accttcgccg accaggcggt
     2401 gatcgcgatc gagaacgtgc gcctgttcaa cgagaccaac gaaggcctcg agcggcagac
     2461 cgcgaccagc gagatccttc ggatcatctc ggattcaccg ggagacgtga agccgatcct
     2521 cgatgcggtg gccgagcggg cgacgcggct gtgcgatacc gcatcgaccg cgatctatgt
     2581 cctcgaaggc gatgcgatgc gccgcaccgc gttcaacggg ccggccgagc tgatgggcaa
     2641 cgagaccgtg gcctacaccg ccggcacgct cgtcggccgg gcgatcgtgg agagcaaggc
     2701 gatccacgtg cacgacatcg agcgcgcgca agccgaatat ccggtgagct gggaattcgc
     2761 gcgcaagttc ggccgtcacc agacgatgct cgcggtgccg ctgctgcgag aggggcggcc
     2821 gttcggcacc atgctgatgc gccgctggga agtgcggccg ttcactgaca agcagatcgc
     2881 gctctccaag gtgttcgccg accaggcggc gatcggcatc gagaacgtgc gcctgtttaa
     2941 cgagacgaag gaagcgctcg agcagcaaaa ggcgtccgcg gacgtgctcg gcgcgatctc
     3001 gagctccatc gcagacaccg caccggtgtt cgacaagata ctggagagtt gccagcggct
     3061 gttcgagggc aacctcgtcg gcgtgacgct cgtggagcac gacgggcagg tgcacctaag
     3121 cgcctacaaa ggggagaaaa gcgaggagct ggcgcggatc tatcccatgc cgctcagccg
     3181 cgaaagcggc accggctgtg ccatcctcga ccagaaagtc gttcagttcg ccgacctcgg
     3241 ccaggacagc gccaccccgc cgcaggtgat caagggcgcg caggtccttg gcttcaggtc
     3301 gatcatcttc gggccgatga tgttcgaagg gcgcggcatc ggcgcgatct gggtggcacg
     3361 caggttcgcg ggtccgttca gcgataagca gatctcgctg ctcaagacct tcgccgacca
     3421 ggcggtgatc gcgatccaga acgcgcgcct gttccgcgag atccaggaga agagcgctca
     3481 gctcgaggtc gcgaacaagc acaagtcgga gtttctcgcc aacatgtcgc acgagctgcg
     3541 cacgccgctc aacgcaatca tcgggttctc ggaggtgctc tcggagcgca tgttcggcga
     3601 gatcaacgag aagcaggccg actacctgaa ggacatccac gagtcggggc gtcacctgct
     3661 ctcgctcatc aacgacatcc tggatctctc gaagatcgag gccgggcgca tggacctcga
     3721 gatctcgacc ttcgacctgc ccaccgcgct gtcgaacgcg atgacgctgg tgcgcgagcg
     3781 cgcgcagcgc catggcatcg cgctgtcgct cgaggtcgac aagcggctcg gggcgttcca
     3841 ggccgacgag cgcaagttca agcagatcgt gctgaatctc ctctccaacg cggtcaagtt
     3901 cacgcccgac ggcggcagcg tcgacgtttc ggcccggaag cacaacggca agatcgaggt
     3961 cgcggtgcgc gacaccggca tcggcatcgc gccgcaggac cacgccgcgg tgttcgagga
     4021 gttcaagcag gtcgggcgcg actacaccaa gaaggccgag ggcacggggc tcggcctgac
     4081 gctcaccaag cgcttcgtcg agctgcacgg cggcgcgatc agcctggaga gcgcgctcgg
     4141 caagggatcc actttcacca tcacgttacc ggtacgccaa tgaggaactt ccagtgagca
     4201 cagtgctgat catcgaggac aacgaaaaga acatgaagct cgcgcgcgat gtgctgcagg
     4261 cgaagggcta ccagaccctc gaggccgtca ccggcgagga tggcgtgaag cttgcaaagg
     4321 agaggaagcc cgacctcgtg ctcatggaca tccagcttcc cgggatcaac ggtatcgaag
     4381 ccttcaagca gatccgcggc aacgcggcca ccggcaagat cccggtgatc gcactgacgg
     4441 cgtcggtcac gccgaccgac cgcagcgcga tcaacgccgc gggcttcgat gccttcctcg
     4501 gcaagccgat caacctgaag gaattcctcg ataccgtgaa gcgcttggtg gagaaatcga
     4561 aaaagtgacg ccggccaaga ttctggtcgt cgacgacacg ccggcgaacg tcaagctcct
     4621 ctcggacctg ctctcagtga agggctaccg ggtgtgcacg gccgtgaacg gcgaggaggc
     4681 gctcgcgcag gtgacgaagg agaagcccga cctcgtgctg ctggacgtga tgatgccggg
     4741 aatctccggt tacgaggtct gcaagaagat ccgcgagaac cccgagaccg cgctgctgcc
     4801 ggtggtgatg tgcacttcgc tcgacccgaa ccaggagcga gtgaagggca tcgaggcggg
     4861 agcggacgac ttcctctcca agccggtcaa ccagccggag ctgttcgcgc gcgtgcgctc
     4921 gctgctgcgc atcaagggtt tgcaggacga acgccttgcg cgcctaaaaa gcttcttttc
     4981 gccgcaactc gccgaggcga tcgccgccgg caagggcgag gacctcctca agacccatcg
     5041 gcgcgagatc acggtcgagt tcttcgacct gcgcggcttc acccacttca ccgagaccgc
     5101 cgagcccgag gaagtgatgg acctgctgcg cgcctaccac aacgccctcg gcaagctggt
     5161 cctcgagcac cagggcacgc tcgagcgctt cgccggcgac gcggtcatgg tgttcttcaa
     5221 cgaccccgtg ccgatcgacc ggcccgccga gcacgcggtg cgcaccgcgc ttgccatgca
     5281 cgaagccttc gtcccgatcg gcaagcactg ggagaaacgc ggctacgagc tcgcgctcgg
     5341 cgtcggcatc gcccagggct acgccacgct cggcgccatc ggcttcgagg ggcgctggga
     5401 ctacgccgcg atcggtaacg tcaccaacct cgccgcgcgc ctgtgcgccg aggccaaggc
     5461 cggccaggtg ctcctagacc gcaagaccat ggccgcgctc gacggcctgt tcgacttcga
     5521 tcccgtcggg ccgctgacgc tcaagggctt ctcgcagccg gtgccggcct tcgcgctcaa
     5581 gaagccgtga catgggaaag ccccgtcgct ccacgcgcac tggcaagaag gcggcgccgc
     5641 ggcgcatcga gcgctctcgc cgcgcccatc ctgcggccgc gaagcttgcg gtagcgctgc
     5701 gggaatacaa ggaagtgctc gagcagcagg cggcgaccgc ggacattctt cgcatcgttg
     5761 ccgcatccgt cgagaatgcc gacccggtgt ttgccgccat aacccgcgcc gggcagcacc
     5821 tggtatccgg ctctcgcgtg gggctcctcg tcgtccgtga tggacagctg caccaggccg
     5881 cgtacagcgg catccctgat gagcaagtgg ccattctggc gaagcacttt cctgcgccgc
     5941 tcgaccgcca gaccatcgcg ggagcctcga tcctcgacag gcgagtaatt caggtccccg
     6001 atatcgccga agtgcgggag cgctttcccc gcagcttccg gtcagcccgg tcatccgggg
     6061 accgggcgtt acttgtggtc ccgctcctgc acggcgaaag cgcggtggga tcgctctcga
     6121 taaggcgcac gaaaccgggg gcttttactg cgaggcaggt cgagctggcc cgaacattcg
     6181 ccgaccaagc ggtcatcgcg atccggaacg cccgcatgtt caacgagacc aaggaggcgc
     6241 tcgagcagca gaccgcgacc gccgagatcc tcaaggtcat cgccggttcg ccgaccgagg
     6301 tgcagccggt gttcgacgtc attgttgagc gcgcggtgcg cctgtgtggg gcgcgcttcg
     6361 ggcgggtcta ccgctacgac gggtcaacca tccacatggt ggcgagccac ggcctgagcg
     6421 ccaaggggct cggccaggtg cagcgcgtgt tcccgcggcc cgccgcggac gacaccatcg
     6481 tggggcgcgt gatcctggcg cggcagccgt acttcatcga ggacgtcgag cgcgaccaga
     6541 gcgtaccctc gttgtcgcgc cagatgatag aggcgctcgg cacgcgccgc cagctgacga
     6601 ttcccatgct gcgcgccgga gagccgatcg gggcgatcac gatgggctgg gacaagccgg
     6661 ccggcttcga cgaccaggag gcgtcgctgc tcaagacctt cgccgaccag gcggtgatcg
     6721 cggtcgaaaa cgtgcgcctg ttcaacgaga ccaaggacgc gctcgagcgg caaacggcta
     6781 ccgccgagat cctcaaagtc atcgcgagct cgccatccga cgttcaacct gtcttcaatg
     6841 cgatcgcagc gagcgcaaaa caactcttgg gcggtttgag cggcgttgtt acccgcgtgg
     6901 atggtggcat gcagcatctc gctgccttca ccacgacggg cgaatccggc gacgaaacct
     6961 tgcggcgtct gtttccgatg ccccgcggcg agctgatttc cggcagggcg atcacgacgc
     7021 tggcgccggc aattcgcacg gacacggaaa cggacccctc catttcagag gcggcgagag
     7081 agaacgcccg tgcacggggg tatcgaagcg cgcttgcggt gccgatggtt cgcgaaggcg
     7141 tcgccatcgg ggcgatttcc gtggctcgcg ggataccggg gtcgttttcg gatcatcaga
     7201 tcggtttgct gcagaccttc gcagaccagg ccatcatcgc tatcgagaac gtgcggctgt
     7261 tcaacgagac caaggaagcg ctggaccggc agacagcgat cagcgagatc ctcgagaaga
     7321 tctccagctc cccgacggat gtgctgcccg tgctccaggc ggtcgcggca cgcgccgcgc
     7381 acctgtgcga gggcgagcag gcgacggtgc tgatggtcga aggcgacatg ctgcggcccc
     7441 gggtcaccta ttcgagcgac caggggccgc tgccgaatcc ttcgaccctg gtgtacctga
     7501 agcgcggcta catcaccggc cgcgcggcgc tcgatgcgga agcgatcaac gtcgaggacg
     7561 cggccgcgct catggacagc gagtacacgg acggccggga gaatcagcag aagctcggct
     7621 accgcagctt cctcgcggtt cccatgatgc gcgagggcaa ggcgatcggc gtcatcgccg
     7681 tctggcgccg cttcgtgcgc aagttctccg acaagcatgt tgcgctggtg aagaccttcg
     7741 ccgaccaggc ggcgatcgcg atcgagaacg tgcggctgtt caacgagctg caggcgcgca
     7801 ccgaggcgct cacgaagtcg gtcaggcaac tcacggcgct gggcgaggta gggcaggcga
     7861 tcagctcgac gctcgacctg gagacggtgc tgaagaccat cgtttcgcgc gcggtgcagc
     7921 tcaccggcct ggatgccggt gcggtctacg aatacgacga gcgcgcggag gagttccacc
     7981 tgcgcgcggc ggagaacatg agcgaggaga tcatcgagct cctgcgcagc acgccgatca
     8041 agaaaggcga cggcgcggtc ggcgccaccg cggtggcgcg cgagccgatc cagattcccg
     8101 agatccggga cgagggcttc aagagccagt ttcgcgaggt gctgatccgg acgggacggc
     8161 gggcgcttct cgccgtaccg ctgatgcgcg aggaccacat catcggggcc ctggcggtgc
     8221 agcggaacac gccggggccg ttcgcgcccg aggtggtcga ggtgctcaag acgttcgcca
     8281 cgcattccgc catggcgatg cagaacgcgc gcctgttccg ggagatcgcc gagaaagggc
     8341 ggcagctcga agtcgcgagc gagcacaagt cgcagttcct cgccagcatg agccacgagc
     8401 tgaggacgcc gctcaacgcg ctcctcggct tcaacgagat gattctgggc gacatctacg
     8461 gcgaggtgcc gggcgacatg cgcccgccgc tcgcgcagat gcaaacgagc gggaagcacc
     8521 tgctgcgcct gatcaacaac gtgctcgatc tcgccaagat cgaggccggg cgcatggagc
     8581 tcgcgctcgc cgactactcg gtgcaggatg tcgtagaagg agtgcgctcc acgctgcaac
     8641 ccctcgcggt ggagaagggc ctcgagttcc tggccgcagt gccggctgac gttcctctcg
     8701 cgcacggcga ttccgggcgc atcacgcagt gcctcatgaa cctcgccggc aattcgctca
     8761 agttcaccaa ggccggcaag gtcgagatct cggtggtgat gaatagcggc cagctgcgtt
     8821 actgcgtcgc cgacacgggc atcggcatcc cgcgggacaa gatcggcagc ctgttcacgg
     8881 agttcaggca gaccgacgcc accgtcgcga gcgaatacgg cggcaccggc cttggcctgt
     8941 cgatcagcaa gaagttcgtc gagatgcacg gcgggcgcat ctgggtcgag agcgagccgg
     9001 gacgcggctc ggtgttcacg ttcgaggtcc cgcttcgggc ggcggcggca tgagcgcggc
     9061 cacgatcctg tacgtcgagg acaacgaggc gaaccgcatg atcgtgcgcg acctgctgaa
     9121 gcgcacgaag tacacgctga tcgaggcggt cgacggggag gcgggcgtgg cgaaggccct
     9181 ggaggcgcgc ccgggcctga tcctgatgga catccagctg ccgaagatct ccgggctgga
     9241 ggcgatccgg cgcctgcgcg ccgagcctgc gaccgcggcg acgccgatca tcgcgatcac
     9301 ctcgttcgcg ctctccggcg acgagcagaa ggcaaaggcg gtcggcgcca ccgcctacct
     9361 cgcgaagccg tacagtccgt tcgacctgct caagctcgtg cgccagtacc tgcccgaatc
     9421 atgaagcggc gcaaggagcc gagcgccgga gtcgtacgcg acggtcgccg ggaagcggcc
     9481 gaagcgatca agcgggaggc cgcgaccgcg gcgattctca aggccatccg ccgctcacct
     9541 ggcgacgcgc agcccgcgtt cgacgcgatc gtgcgcagcg cgctgcgcct ggtgaacggc
     9601 cactccgcgc tggtgctgcg gctcgccgac ggcatgctgc acctcgccgc gcacacctcg
     9661 accggccgat ccggcgacga ggccatccgg cgcttctacc ccaggccggt cgccgaggtg
     9721 actctgcatg gccgggcgat ccgatcgcgc cgtccctgtt gcgtcgccga cactgaaaaa
     9781 gtttccgcct cgatggcggc gatgaaggag atggcgcgga agaggggtta tcgcagcctg
     9841 atggccgtgc cgatgctgcg cgacgcagtc ccgatcggct ccatcggcgt gtcgcgcaag
     9901 caggccggcg cgttcagcag gcaccagatc cgcgcgctcg aaaccttcgc cgaccaggca
     9961 gtgattgcaa tcgagaacgc gcgcctgttc aacgagacca aggaagcgct cgagcggcag
    10021 accgccaccg ccgagattct gcaggtcatc gcggcctcgc cgtccgacac gcagccggtg
    10081 ttcgatgcaa tcgcgcgcag cgcgcagaag ctgctcggcg cgcattatgc gaacgtgacc
    10141 cgggtcgtcg agggcgcgct gcacctcgcc gcgcacaccg ggaccaaccc ggctggcgtc
    10201 gaatctctca agaaggtgtt cccggcgacg ctggccgggg agggcgcgct cggcagggcg
    10261 gcgctcagcg gcgagccggc ctgcattgcc gatatcgaaa ccgatccggc gtactccgcc
    10321 tccttccgcg agggggcgcg cgtgcgggga taccggagcc tgcttgccgt gccgatgctg
    10381 cgcgacggag tgtcgatcgg cacgatcgcc gtcacccgcc gggaccccgg accgttctcc
    10441 gatcaccaga tcggcctgct caagaccttc gccgaccagg cggtgatcgc gatcgagaac
    10501 gtgcgcctgt tcaacgagac gaaggaggcg ctcgagcagc agacggccac gtcggaaatc
    10561 ctgaaagtca tcagttcctc gcctaccgcg acgcaaccag tgttcgagtc gatcgtgaag
    10621 aactgccatt ccctgttcgg cgacagccgg gtggcagttt ggcagatcag cgacggccgg
    10681 ctcgttcccc gagcaagcac gggttatttt ccggagccga tgccggtcga cggggagagc
    10741 ggcatcggag cgtgcgtcct cgaatcgcgc accatccatc tacccgactt gcgatcgggt
    10801 gctgagcggt acccgcgcat caagcagctg ggcctgaagc acggttacct ctcgggcgtg
    10861 tacgcgccgc ttctgcgcga aggccaggcg atcggcggta tctcgctgct tcgccgccag
    10921 cccggcgcgt tcagcgacaa ggaggtcgcg ctgctcggca cgttcgccga ccaggcggtg
    10981 atcgcgatcg agaacgtgcg cctgttcaaa gagaccaagg aggcgctcga gcagcagaag
    11041 gctgccgccg acgtgctcag ggtcatcagc agctcgccca ccgacatcat gccggtatac
    11101 cggacgattc tggagaacgt tacccggctg tgcgaggcga acatcgccgc gcttttcctg
    11161 tacgacggcg agttcatccg gacggccgct caccggaacg cgacaccgga gttcgcacag
    11221 cagctagatg cgctgcgcgt gccgccgagc cgtgagacgc cgacgcggcg cgctgcgttg
    11281 gagaggaaga tcgtgcacgt cgaggacgtg ctgagcgatc ccagcttctc gctttccgaa
    11341 gcgcaccgcc gcgagcagcc gcggaccgtg ctctcggcgc cgctgctgcg cgagggcggc
    11401 cttgtcggcg tcatcacggt ctggagacgc gagccgcgcc tgttcaccga caagcaggtc
    11461 gcgctggtga agaccttcgc cgaccaggcg gtgatcgcga tcgagaacac ccgcctgttc
    11521 caggaactgc aggcgaagaa cgacgacctg cgcgaggcgc ttgagcagca gaccgccacc
    11581 ggcgacatcc tgaaagtgat cagccgctcg accagcgacg tgaagccggt gttcgagacc
    11641 attatccgca gcgccgtgcg gctttgcgaa ggcgccttcg gtacggtgtt cacgttcgac
    11701 ggccaggtgc tcgacatcgg cgcggagtac aactacagcg cggacgtgac ggagatgttt
    11761 cgccgcgagt atccgcagcc cgcggaccgc tcgacgcctt cgggaagggc tatcgtcgag
    11821 ggcagggtga acaatatccc cgacatcgcc gagtcggatt actcccaagc ggtcagggac
    11881 cgggcgcaag ccaacgggtg gcgcgggttg ctgaccgtgc cgatgacgcg cgccggcgtc
    11941 gcgatcggcg cgatcgcggt ggcgcggcgc gaggcgggcg tctttgacga ctcgtacgtc
    12001 gcgctgctgc agaccttcgc ggatcaggcg gtaatcgcga tcgagaacgc gcgcctgttc
    12061 aacgagacca aggaatcgct tgagcagcag accgcgaccg ccaagatcct cggtgtcatg
    12121 tcgtcttcac cgaccgacgt gcaaccggtg ctcgatgcgg tcgccgaaag cgccgcgcgc
    12181 ctgtgcgaga ccgacgacgc catcatccgg cgcgtcgact cggacggtct gcggcgcgtt
    12241 gcgcatttcg gcccgctgcc ggcaacgccg gagaccgaga tccatccgct cgtcgccggc
    12301 agcgtcggcg gccgtgcggt gctcgagcgc cgcgcgatcc atgtcgagga tatcgaggaa
    12361 gaggtgcgcc agggacgcta tcccgaggcg agttccctgg cggcggccgc gggctggtac
    12421 cgctcgatgc tcgccatgcc gctcatacgc gaggacacgg cgatcggcgt catcatgatc
    12481 cggcgcatgg aatatcggcc gttcaccgac aagcagatcg cgctgctcag gacgttcgcc
    12541 gatcaggcgg tgatcgcgat cgagaacgtc cggctcttca acgagacgaa ggaggcactc
    12601 gagcagcaga ccgcgatcag cgaggtgctg cgggtgatct cgagctcgcc ggccgacgtg
    12661 aagccggtgc tcgacgcggt ggccgagcgt gccgcgcgga tctgctcggc gagcgacgcg
    12721 aggatcttcc tggtggaagg cgagcaccta cggcatgtgg cgggtttcgg cgacgtgggc
    12781 gcgcctgagg tcggtgttca gcggctgatc gagcccggga tcgtcatggg ccgggcggtc
    12841 atcgatggca ggccgatgca cgtcgaggat attcaggcgg tgccgcatga atttccaacc
    12901 ggggcgcaat acggcaccca gttcggctgg cgcaccatcc tcgtcgtgcc gctgatgcgg
    12961 gagcagcgcg cgctcggcgt catctcgctg cgcag
//
LOCUS       DATBSZ010000030        17091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1983943, whole genome shotgun sequence.
ACCESSION   DATBSZ010000030 DATBSZ010000000
VERSION     DATBSZ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17091
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983943"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VIG70_01790"
     CDS             complement(<1..971)
                     /locus_tag="VIG70_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEY8249309.1"
                     /translation="MHLLYEEDGELKAGTVLERSPASFHVESPHGRRSKIKAASVLLS
                     FERPGAAELLGEAEKFSRDLEIHFLWECSGAREFGFQDLAREYVGREPTPAEAAGVLL
                     KLHSAPMYFYRRGKGRYQPAPEATLKLALAAVEKKKRVQEQVAAWAERLAKGDCPAEI
                     ASLKDELLYAPDRNKPQTRALEQACAQTGLTAARLFERCGLLSDSHDYHLKRFLHELF
                     PRGIAFPAHEVPSAPADLPLAPAGAFSLDDIGTTEIDDAFSVQRLTAGVRIGVHIAAP
                     ALGFAPGSPLDAIARERLSTAYMPGRKFTMLPEDVIERFSLDHGGELP"
     gene            complement(955..1515)
                     /locus_tag="VIG70_01795"
     CDS             complement(955..1515)
                     /locus_tag="VIG70_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEY8249310.1"
                     /translation="MIVYLHGFNSSPQSHKAQVMQRFMAERGLPHEFACPALPPLAGD
                     AMKAIAPLVSKGSCLVGSSLGGFYATYLAEKHGLKAVLINPAIDPHIGLRAYLGPQKN
                     LHSGEPYELTEAHLREWEALYVPRITPQRYLLIVETGDEVLDYRRAVARYAGAEQVVI
                     PGGDHSLQSFPQHLARILEFSRAPSV"
     gene            complement(1490..2848)
                     /gene="mpl"
                     /locus_tag="VIG70_01800"
     CDS             complement(1490..2848)
                     /gene="mpl"
                     /locus_tag="VIG70_01800"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEY8249311.1"
                     /translation="MPAHLHILGICGTFMGGIAAIAKSAGYRVTGCDANVYPPMSEQL
                     RALGIELTPGFGAEQLALRPDVWVVGNVVTRGNPLMEAILDAGERYVSGPQWFAEEVL
                     AGKHVLAVAGTHGKTTVSAMLAWILEKAGRSPGFLIGGVPNDFPVSARLTASPLFVIE
                     ADEYDTAFFDKRSKFVHYRPRTAILNNIEFDHADIFDDLASIERQFHHFVRTVPRSGK
                     VIANGTEETVKRVLTLGCWTPVEAFGVVDGWQAGEADAQGAFEVSFAGQGLGRVQWSL
                     LGTHNRFNALAALAAARHVGVEPAAGIKALGSFGGVRRRMQVRGEAKGVTVYDDFAHH
                     PTAIRTTLEGLRQRVGKARILAVLDPRSNTMKRGVMKDALPPSFAQADRVYIYTSGIG
                     WDARSLFALLGPRARCEEDLEALIAAVVGEARAGDHVLVMSNSGFGGIHGKLLDRLSA
                     RV"
     gene            2882..3520
                     /locus_tag="VIG70_01805"
     CDS             2882..3520
                     /locus_tag="VIG70_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249312.1"
                     /translation="MSRKGTRQTGMRARSQEMRARIAATAARIMAEDGIDDFALAKRK
                     AARQLGASQTEALPGNDEIEAELEAYRALYQPEEHPERIAELRRIALDAMQALERFSP
                     YLTGPVLKGTAGPYAEIELQLFPESAKEVELFLLEQRIAFDTHEGRRFSGDRVHAMSV
                     FTLSWQDAPLKLSVFDPRDERLALKTSQAGRVMERAGIAEVGALLRNGEQAA"
     gene            3501..4028
                     /locus_tag="VIG70_01810"
     CDS             3501..4028
                     /locus_tag="VIG70_01810"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8249313.1"
                     /translation="MASRRRDALILGTAGVAAAAAGALAGVFGLQARSGAAQLLGARF
                     TDLDGRSRRLLDAAKGRALLCNFWATWCAPCREEIPLLVAAKQELTSTRVEIVGIGID
                     SVDKIRQFAATYKINYPLLVADATAVSLLHALGNKSGGLPYTVALDGSGALTERRLGA
                     LKEADLRQVLARLVG"
     gene            4074..4523
                     /gene="accB"
                     /locus_tag="VIG70_01815"
     CDS             4074..4523
                     /gene="accB"
                     /locus_tag="VIG70_01815"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY8249314.1"
                     /translation="MDLRKLKKLIDLVEESGIAELEITEGEEKVKIVKGGVVTVAAAQ
                     PVALAAAPAAAVAVPAAGPATEPEPGQEGHVVKAPMVGTFYRSPSPDAKVFVEVGQAV
                     KEGDTICIIEAMKLMNEIEADASGVVKAILVENGQPVEYGQPLFILG"
     gene            4524..5882
                     /gene="accC"
                     /locus_tag="VIG70_01820"
     CDS             4524..5882
                     /gene="accC"
                     /locus_tag="VIG70_01820"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY8249315.1"
                     /translation="MFGKILIANRGEIALRIQRACRELGIRTVVVHSEADTEAKYVKL
                     ADESVCIGPPPAAASYLNIPAIISAAEVTDAEAIHPGYGFLSENADFAEKVERSGFVF
                     IGPRPENIRLMGDKVSAKAAMTKAGVPTVPGSEGALPEDPKEVVRIARKVGYPVIIKA
                     AGGGGGRGMRVVHTEAALLNAVNMTRAEAQAAFANPVVYLERYLEQPRHIEIQILADA
                     HKNAVHLFERDCSLQRRHQKVLEEAPAPELPTKLRDKIGERCVDACRRIGYRGVGTFE
                     FLYQGGEFYFIEMNTRIQVEHPVTEMITGVDLVQEQIHVAAGERLRLRQKDLAVRGHA
                     IECRINAEDPYRFTPSPGKITSYHPPGGPGIRVDSHVYQGYTVPPNYDSMVGKVIAFG
                     ATRDQALGRMRIALSEMVVEGIQTNIPLHRELLNDTRFMRGGVSIHYLEQKLAQEEKK
                     KK"
     gene            5899..6702
                     /locus_tag="VIG70_01825"
     CDS             5899..6702
                     /locus_tag="VIG70_01825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY8249316.1"
                     /translation="MPWLALTLQVERAAAEAFSDALLEAGAQSVSLETAGAVTALLSV
                     EDDSKTLVATAARAAGIAPPGFSVETLHDQDWVRASQAQFAPVAVGERLWIGPSWHAA
                     PPGRIAVRIDPGLAFGTGSHPTTRLVLRFLEKNIKGGERVLDYGCGSGILAIAAARLG
                     AAHVDAVDLDPQAVETARANAAANRVVLNCALPDALPAAGYDMVVSNILAQPLIALAP
                     MLAPCGARIALSGILDAQAGEVAGAYEPWFDMGIDDRDDGWALLVGSKK"
     gene            6699..7472
                     /locus_tag="VIG70_01830"
     CDS             6699..7472
                     /locus_tag="VIG70_01830"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HEY8249317.1"
                     /translation="MNLVTRCPACGTAFRVQRDQLAAHFGTVRCGKCGGVFNGVAALV
                     EEGAEKLSLDPSPQLGLFDPTRRPAQAAGMRDDAPLPEFLAGERAARPRLWLWLLLSL
                     AAAAMLGAQLYLSRNEATSWLPELRPAFTLVCRLAGCSVALPRRPELLKIVASDMHDD
                     SARQVFVLNATLRNLARFPQQYPDLELTLTNAGKPVTRRVLGPEEYLDSRVASRVIST
                     GLEAGAEEPLRVHVDAKLVRATGYELCIYPQDCLKHATK"
     gene            7469..8413
                     /locus_tag="VIG70_01835"
     CDS             7469..8413
                     /locus_tag="VIG70_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY8249318.1"
                     /translation="MKKILVTGSIAYDTIMVFPDRFRNHLLADQLHILNVCFLTPEMR
                     REYGGTAGNIAYNLKLLGEDALVMATVGEDIQPYLQRFEALGIGTRPLRRIPGQFTAQ
                     AFITTDLDDNQITAFHPGAMNHSHQNHVTRELGAGLAIIAPDGKDGMLQHARECASAG
                     VSFMFDPGQGLPMFSGDELGEFVRLADYVAVNDYEGKMLEEKTGRKLEALAREVKALV
                     CTLGAKGSVILAQGQRHEVPSAPADAVVDPTGCGDAYRAGLLYGIAHGWDWPSTGKLG
                     AVMGALKIAHRGAQNHVATRAEIEGRFRKAFGYSPWQG"
     gene            complement(8396..8812)
                     /locus_tag="VIG70_01840"
     CDS             complement(8396..8812)
                     /locus_tag="VIG70_01840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY8249319.1"
                     /translation="MSDCVFCRIVARQIPATVVHEDEHTLAFMDLGQVNPGHVLVTVK
                     AHAENLYALNDAQAGAVLRVAAKVGRAIRDAFAPHGLSVYQANGKAAGQTVFHYHVHL
                     VPRHEGDGMALTWPVKNPPREKLEDYAAKIRSALPR"
     gene            complement(8809..9624)
                     /locus_tag="VIG70_01845"
     CDS             complement(8809..9624)
                     /locus_tag="VIG70_01845"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEY8249320.1"
                     /translation="MATYAIGDVQGCFDELRQLLAKVGFKKQDRAWFVGDLVNRGPKS
                     LEVLRFVRDLGERAVVVLGNHDLHLVAQYEGIEKIRRADTFQDVLDAPDAAELLAWLR
                     RQPMVHAEGGYAMVHAGLLPQWSIAKAKRLGREVSAALAARDYRTFLEHMYGNTPSRW
                     RDSLSGWDRLRVIVNAMTRMRYCDRAGRMDLSRAGIEPARGYRRWYETRKRERGKTLI
                     FGHWSQLGLVLEPGTIGLDSGCIWGGALSALRLEDRTLYQAACPGYQSPGGEA"
     gene            complement(9649..11148)
                     /locus_tag="VIG70_01850"
     CDS             complement(9649..11148)
                     /locus_tag="VIG70_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY8249321.1"
                     /translation="MTVATVASRALAGIDAPEVTVEVHLGPGLPAFHIVGLPDAQVRE
                     ARDRVRAALNHVHFEFPARRITVNLAPAELPKDSSRFDLPIALGILAASGQLAPQALA
                     GQEFAGELSLTGELRGVRGALAMALSARAAGRAFVLPEGNAPQAALAEGARILPARTL
                     LEVVSHLAGEKPLEQYGAEARCASVAYADFSDVKGQQQAKRALEVAAAGAHSVLMIGP
                     PGTGKSMLAARFPGILPALSEAEALEVAAIHSVSASGFEAARWGERPYRAPHHSASAA
                     ALVGGGVLPRPGEISLAHHGVLFLDELPEFDRDVLEALREPLESGRVSIARAARQAQF
                     PARFQLIGAMNPCPCGHCGDRSGRCRCTPERIARYRGRISGPLADRIDIKLEVPPPRE
                     SELVAPVSGEASEAIRGRVESARKLQASRQGKPNALLGTREIDRHCATDREGDQLLRH
                     ALARLLLSARAYHRVLRVARTIADLAQKTSISGEHIAEAIQYRRLDASF"
     gene            complement(11195..11434)
                     /locus_tag="VIG70_01855"
     CDS             complement(11195..11434)
                     /locus_tag="VIG70_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEY8249322.1"
                     /translation="MIDPKLFDDLRARIDEALRGSPAQDIEKNLRALLAAWFDRLDLV
                     LREDFEVQKKLLERAQAKLTELEARIAELETRKSR"
     gene            11609..12364
                     /locus_tag="VIG70_01860"
     CDS             11609..12364
                     /locus_tag="VIG70_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEY8249323.1"
                     /translation="MRKSILTLSVAAALAAPGLVAAQTAPAPAAAPSPVTGNMAIVSD
                     YRFRGISQTFELPAIQGGIDYAHQSGIYLGNWNSSVSGLSFTDTAGIEMDFYGGWKKS
                     FGDIGFDIGLLQYYYPGAKTGGGEEYNTLEGYIGASWKWLTLKYSYTFSDYFGLVNSD
                     GSGYLDLGFTMEVAPKLTIVAHYGMQTIQNYGALDYNDWKLGVTYDLAGWILGAAYID
                     TDADSGLYTLTNSKGKSKDLGGSTVVLSIMKTF"
     gene            12372..12710
                     /gene="glnK"
                     /locus_tag="VIG70_01865"
     CDS             12372..12710
                     /gene="glnK"
                     /locus_tag="VIG70_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY8249324.1"
                     /translation="MKLVTAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEAAVKSDLLEQALEAIERSANTGKIGDGKIFVFDLEKVIRI
                     RTGETDAAAL"
     gene            12721..14193
                     /locus_tag="VIG70_01870"
     CDS             12721..14193
                     /locus_tag="VIG70_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY8249325.1"
                     /translation="MKKFCAFLALWLSLAFAGATLAQDKPAATPDKPAAAEVKKEEAK
                     PAPPTPNKGDVTWMLMSTALVIMMSVPALALFYGGMVRSKNALSVLMQVFVVFSLVTV
                     LWCIYGYSLAFTEGNAFFGSWKDRLFLSGALDAGKGEFANAATFSKGVVIPEIVFVAF
                     QATFAAITCCLILGAFAERAKFAGVLAFIVLWFTFAYTPVAHMVWFWMGPDAYTSAKV
                     VDEMNAKAGWLWQMGALDFAGGTVVHINAGVAGLVGAYLVGRRIGYGKEAMPAHNLPL
                     TMIGASLLWVGWFGFNAGSALEAGNSAALAFLNTYLATACAVLSWSLGEAMFKGKPTM
                     LGAASGAVAGLVAITPAAGNVGIPGAFVIGLSAGLVCLWGVSGLKKLLGADDSLDVFG
                     VHAVGGILGALLTGVFNSPGLGGPGFVNNWFEMKQGFESIGGQLWIQAKAVGVTILWS
                     GIVALVAYKLVDWTIGLRVAEEEEREGLDTASHGEAAYRM"
     gene            complement(14247..14660)
                     /locus_tag="VIG70_01875"
     CDS             complement(14247..14660)
                     /locus_tag="VIG70_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8249326.1"
                     /translation="MLKPGPLPGLLGYRLRLAQQAVFRDYAAAVPEASPGRAGILFLI
                     EANPGVTQSRLAQAVGLDRSTMVGVLHALEARELVERRRGEDRRTNGLWLTRAGRALV
                     ASLKRRIRVHERRVASRLTAAERTQLLALLEKLAG"
     gene            14722..15711
                     /locus_tag="VIG70_01880"
     CDS             14722..15711
                     /locus_tag="VIG70_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8249327.1"
                     /translation="MQITLVGHDQPVPVQAGDTVLASLLRAGLAFPFSCQAGNCGTCK
                     CELVSGDVHELEYSEHALSAAERARGIVLACRTQVWDDTVVRRIDAEELVVHPSRVMR
                     CRVLEVESLTHDIKGLRLAIEAGGPFTFSAGQYAQVEFAPGLSRHYSMANTPDEPELL
                     FQVRHMPGGRTSSYVATQLKVGDRIKVSGPLGIAYLRDAHRGPVLLVAGGSGLAPIQS
                     ILCTLLERGHESPATLYFGVRSERDLYHEPLLRNLAARHPNFSYHVVLSEQKGASGRR
                     YGLVHEAIEIGDAENAMAYLAGPPVMVEAATAWLAARGLAQRQIHADAFYNQN"
     gene            15715..16608
                     /locus_tag="VIG70_01885"
     CDS             15715..16608
                     /locus_tag="VIG70_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8249328.1"
                     /translation="MDLQGKGAIVTGGARGIGLAIVEELVAQGASVVIVDSGVSISGE
                     PLEPEIAKQVAQRIAGSVAVCGDIADPDVAREAVDLACRKFKSVDIVVNNAAIIRDAF
                     IFKPEPDDWDAVIRTNLSGAQRLLGAATPVMREQVKNGRAPGAIVNIVSTAGIYGNFG
                     QGAYGAAKAGLIGLTRVVAHDLARSKIRCNAIAPFAATRVTESIKPANEAQAAYKAKA
                     LRVPARHVARLVSLLASDRAAHVTGQLFGVRGREVFVFSQPRPAQRMLQTDQGFDLKA
                     LEPHFTALVTDLEAFSGDPVL"
     gene            16619..>17091
                     /locus_tag="VIG70_01890"
     CDS             16619..>17091
                     /locus_tag="VIG70_01890"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEY8249329.1"
                     /translation="MSAYPSIKTVEEFRQQPEAYQTAVRKIVRSHALNELYGAQVFDE
                     PAIGLAPTPYAKWLTCRVAMEEYHHHVRFRELADQIGVRAEEMDARNKRPLNIFEVPL
                     ESWEQFCVIKMLGDLAEILQVEDLLHCSFVPLRNIARTTMPEEKFHAQFGLDFCV"
BASE COUNT         2851 a         5741 c         5763 g         2736 t
ORIGIN      
        1 ggcagctcgc cgccgtggtc gagcgagaaa cgctcgatca cgtcctcggg gagcatggtg
       61 aatttgcgcc cgggcatgta ggcggtcgag agccgctcgc gtgcgatcgc gtcgagggga
      121 gagcccgggg cgaagccgag cgccggcgct gcgatgtgga cgccgatgcg cacgccggct
      181 gtgagccgct gcaccgagaa cgcgtcgtcg atctcggtgg tgccgatgtc gtccaggctg
      241 aaggcgccgg cgggcgctag cggcaggtcg gcgggcgcgc tcggcacctc gtgggcgggg
      301 aacgcgatgc cgcgcggaaa aagctcgtgc aggaagcgct tcaggtggta gtcgtggctg
      361 tcgctcagga gcccgcagcg ctcgaaaagc cgcgccgccg tgaggccggt ctgcgcgcag
      421 gcctgctcga gcgcgcgcgt ctgcggcttg ttgcggtccg gcgcatagag aagctcgtcc
      481 ttcagggaag cgatttcggc gggacagtcg cccttcgcca ggcgctccgc ccacgccgct
      541 acctgttcct ggacgcgctt tttcttctcg accgccgcga gcgcgagctt gagagtggcc
      601 tcgggcgcag gctggtagcg gcccttgccg cggcggtaga agtacatcgg cgccgagtgg
      661 agcttcagca gcaccccggc cgcttcggcg ggggtcggct cgcgcccgac gtactcgcgc
      721 gcgagatcct ggaagccgaa ttcccgcgcg ccgctgcact cccagaggaa gtggatttcc
      781 aggtcgcggg aaaatttctc ggcctcgccg agcagctccg cggcgcccgg ccgctcgaac
      841 gaaagcagca cgctcgccgc cttgatcttc gagcgcctgc cgtgcgggct ttcgacgtgg
      901 aaagacgccg gcgagcgctc gagcaccgtg ccggctttca gctccccatc ttcttcatac
      961 agaaggtgca cgcgagaact cgagtatgcg cgcgaggtgc tgcggaaagc tctgcaggct
     1021 gtgatccccg ccggggatga caacctgctc cgcgcccgcg taacgcgcca cggccctgcg
     1081 gtagtcgagc acctcgtctc cggtctcgac gatgagcaaa tagcgctggg gcgtgatccg
     1141 gggcacgtag agcgcctccc actcccgcag atgcgcctcg gtgagctcgt agggctcgcc
     1201 ggagtgcagg ttcttctgcg gcccgaggta ggcgcgcagg ccgatatgcg gatcgatcgc
     1261 cgggttgatc aggaccgcct tgagcccgtg cttctcggcg agataggtcg cgtagaagcc
     1321 gccgagggag gaaccgacaa ggcacgagcc ctttgaaacc agcggggcga tcgccttcat
     1381 tgcatccccc gccagcggcg gcaaggcagg acaggcgaac tcgtgtggga ggccgcgctc
     1441 ggccatgaag cgctgcatca cctgcgcctt gtgagactgg ggcgaagaat taaacccgtg
     1501 cagatagacg atcaagcaat ttgccgtgga tgccgccgaa gccgctgttg gacatgacga
     1561 gtacgtggtc ccctgccctg gcctcgccga cgaccgccgc gatcagggcc tcgaggtcct
     1621 cctcgcagcg cgcccgggga ccgagcagcg cgaacagcga gcgcgcatcc cagccgatgc
     1681 cggaggtata gatatagacg cggtccgcct gggcgaagct cggcggcagc gcgtccttca
     1741 tgaccccgcg tttcatggtg ttcgaacgcg gatcgagcac cgcgagaatg cgcgccttcc
     1801 ccacccgctg tcgcaggcct tccagcgtgg ttcggatggc ggtgggatgg tgggcgaaat
     1861 cgtcgtagac ggtcacccct ttcgcctcgc ctcttacctg catccggcgc cggacgccgc
     1921 cgaaggagcc gagcgccttt atccccgcag cgggctccac tccgacatgc cgcgcggcag
     1981 ccaaggcggc gagcgcgttg aacctgttgt gggtgccgag cagcgaccat tgcacgcggc
     2041 ctagcccctg ccctgcaaag gacacttcga aagcgccctg cgcgtcggcc tccccggcct
     2101 gccagccgtc gacgacccca aaggcttcaa ccggggtcca gcagcctaac gtgagcactc
     2161 gcttcacagt ctcctcggtc ccgtttgcga tcacctttcc ggaccgaggc acagttcgca
     2221 cgaagtgatg aaattgccgt tcgatcgagg caagatcgtc gaagatgtct gcatgatcga
     2281 actcaatatt attgagaatt gcggtccgcg gccgatagtg gacgaacttc gagcgcttat
     2341 cgaaaaaagc agtgtcgtac tcgtccgcct cgatgacgaa gaggggcgag gccgtcaggc
     2401 gggcggagac gggaaagtcg ttcggcacgc cgccgatgag gaacccgggc gagcgaccgg
     2461 ccttttccag gatccaggcc agcatggcgg agacggtggt cttgccgtgg gtcccggcca
     2521 ccgcgagcac gtgcttgccg gccagcacct cctcggcgaa ccactgcggc cccgacacgt
     2581 agcgctcgcc ggcgtcgagg atcgcttcca tcagcgggtt gccccgggtc acgacgttac
     2641 cgaccaccca cacgtccggg cgaagcgcga gctgctcggc gccgaagccc ggcgtgagct
     2701 cgatgccgag cgcgcgcagc tgctcgctca tcggcggata gacattggca tcgcaaccgg
     2761 tgacgcgata gccggcgctt ttggcgatcg cggcgattcc gcccatgaac gtgccgcaaa
     2821 tgcccaggat gtggaggtgc gccggcatgg agggtcgaga ttctatgatt gaatcgaagc
     2881 cgtgagccgc aagggcactc gccagaccgg aatgcgcgcg cgaagccagg aaatgcgcgc
     2941 gcgcattgca gcgaccgccg cgcggatcat ggccgaggac ggtatcgacg acttcgcgct
     3001 cgccaagcgc aaggctgcgc gccagctcgg ggcatcacag accgaggcgc tgcccggcaa
     3061 cgacgaaatc gaagccgagc tggaggcgta ccgtgcgctc tatcagcccg aggaacatcc
     3121 ggagcgcatt gcggagctgc gccgcatcgc gctcgacgcg atgcaagcgc tcgagcgctt
     3181 cagtccttat ctcaccgggc cggtgctcaa gggcacggcc ggtccttacg ccgagatcga
     3241 gctgcagctc tttccggaga gcgcgaagga ggtcgagctc ttcctgctcg agcagcgcat
     3301 cgcctttgac acgcacgagg gacggcgttt ctcgggcgat cgcgtgcatg ccatgagcgt
     3361 gttcacgctc tcctggcagg acgcgccgct caagctctcg gtgttcgacc cgcgcgacga
     3421 gcggcttgcg ctgaagactt cgcaggccgg ccgggtcatg gagcgcgcgg gcatcgccga
     3481 agtgggtgcg ctgctgcgca atggcgagca ggcggcgtga cgcgctgatc ctcggcactg
     3541 ccggggttgc cgccgcggcc gccggcgccc tggcaggcgt gttcggtctt caagcgcgca
     3601 gcggagccgc acagcttctc ggtgcccggt ttaccgatct ggacggacgc tcgcgtcgct
     3661 tgctcgatgc ggcaaagggc agggcgcttc tatgcaactt ctgggcgact tggtgcgccc
     3721 cttgccggga agagatcccg ttgctcgttg ctgcaaagca agaactgacc tctactaggg
     3781 tcgaaatagt gggcatcggc atcgattcgg tagacaaaat acggcaattt gcggcaacgt
     3841 ataaaatcaa ctatccgttg ctggtggccg acgctacagc cgtgagcctg ttgcatgcct
     3901 taggcaacaa gagcggtggt ctgccctaca ccgtcgcctt ggacggcagc ggagcattga
     3961 cggaacgccg cctcggggcc ctcaaagagg ccgatctgcg acaggtgctg gcaaggttag
     4021 ttggctaaga tgccgcgttc tgcgtcgaat tgcggctaaa aacggggttc tgcatggacc
     4081 tgcgcaagct aaagaagctg atcgacctcg tggaggagtc cggcatcgcc gagctcgaga
     4141 tcaccgaggg cgaggaaaag gtgaagatcg taaagggagg cgtggtcacg gtcgccgctg
     4201 cccagccggt cgccctcgcc gcagcccccg cggctgccgt ggccgtgcct gctgcaggcc
     4261 cggccaccga gcccgaaccc ggccaggaag ggcacgtggt caaggcgccc atggtcggca
     4321 ccttctaccg atcgccttcg cccgacgcca aggtgttcgt cgaggtcggg caagcggtca
     4381 aggaaggcga cacgatctgc atcatcgaag cgatgaagct catgaacgag atcgaggccg
     4441 acgcctcggg cgtggtcaag gccatcctgg tggaaaacgg ccagccggtg gagtacggcc
     4501 agcccctttt catcctcggt tagatgttcg ggaaaatcct catcgccaac cgcggcgaga
     4561 tcgcgcttcg catccagcgc gcctgccgcg aactcggcat ccggacggtg gtggtgcatt
     4621 ccgaggccga taccgaggcg aagtacgtca agctcgccga cgagtcggtc tgcatcggcc
     4681 cgccgccggc ggcggcgagc tacctgaaca tcccggcgat catcagcgcc gccgaggtca
     4741 cggacgccga ggcgatccat cccggctacg gctttctttc cgagaacgcg gacttcgcgg
     4801 agaaggtcga gcgctcgggg ttcgtgttca tcggcccgcg gccggagaac atccgcctga
     4861 tgggcgacaa ggtgagcgcc aaggcggcga tgaccaaagc cggcgtgccc accgtgccgg
     4921 gctcggaggg cgcgttgccc gaggatccga aggaggtcgt gcgcatcgcg cgcaaggtcg
     4981 gctatccggt gatcatcaag gccgcgggcg gcggaggcgg gcgcggcatg cgcgtggtac
     5041 acaccgaagc cgcgctgctc aacgccgtga acatgacgcg cgccgaggcg caagccgcgt
     5101 ttgccaaccc ggtggtctac ctcgagcgct atctcgagca accgcgccat atcgagatcc
     5161 agatcctcgc cgacgcccac aagaacgcgg tgcatctgtt cgagcgcgat tgctcgctgc
     5221 agcgccgcca ccagaaggtg ctcgaggaag cgccggcgcc cgagctgccc accaagctgc
     5281 gcgacaagat cggcgagcgc tgcgtcgacg cgtgccgccg catcggctac cgcggcgtcg
     5341 gcaccttcga gttcctgtac caggggggcg agttctactt catcgagatg aacacccgca
     5401 tccaggtcga gcatccggtc accgagatga tcaccggcgt ggatctggtg caggagcaga
     5461 tccacgtcgc cgcgggcgag aggctgcgcc tgcggcagaa ggacctggcg gtgcgcggcc
     5521 atgccatcga gtgccgcatc aacgccgagg acccgtaccg cttcacgccg tcgcccggca
     5581 agatcacctc ctaccacccg ccgggcgggc ccggcatccg cgtcgattcc cacgtctatc
     5641 agggctacac cgtgccgccc aactacgatt ccatggtggg caaggtgatc gccttcggcg
     5701 cgacccgcga ccaggcgcta gggcgcatgc gcatcgcgct ctccgagatg gtggtggaag
     5761 gcatccagac caacatcccg ctgcaccgcg agctgctcaa cgacacgcgc ttcatgcgcg
     5821 gcggcgtgtc gatccactac ctggagcaga agctcgcgca agaggaaaaa aagaagaaat
     5881 gaaattgaag aagcaatgat gccgtggctc gcgctgactc tgcaggtcga gcgcgccgcg
     5941 gccgaggcgt tcagcgacgc actgctcgag gccggcgctc agtcggtttc gctggaaacg
     6001 gctggagccg tgaccgcgct tctttccgtg gaggatgact cgaaaaccct ggtcgccacg
     6061 gcggcgcgcg cggccggaat cgcgccgccg ggcttctcgg tcgaaacgct gcacgatcag
     6121 gactgggtgc gcgcgagcca ggcgcagttc gcgccggtag cggtgggcga gcggctgtgg
     6181 atcgggccga gctggcacgc ggcgccgccg gggcgcatcg cggtccggat cgatccgggc
     6241 ctcgccttcg gcacgggcag ccatccgact acgcggctgg tgttgaggtt tctcgagaaa
     6301 aacatcaaag gaggcgagcg cgtgctcgat tacggctgcg gctccggcat attggccatc
     6361 gccgccgcca ggctcggcgc ggcgcacgtc gacgcggtcg accttgaccc ccaggcggtc
     6421 gagacggcgc gggccaatgc ggctgccaac cgcgtcgtgc tgaattgcgc gctgcccgac
     6481 gcgttgcctg ccgcgggcta cgatatggtg gtgtcgaaca tcctcgccca gccgctcatc
     6541 gccctcgcgc cgatgcttgc gccgtgcggc gcgcgcatcg cgctttcggg gattctcgac
     6601 gcgcaggccg gcgaggtcgc cggcgcctac gagccctggt tcgacatggg aatcgacgat
     6661 cgggatgacg gctgggccct tctggtagga agcaagaaat gaatctggtc acccgctgcc
     6721 cggcctgcgg cacggcgttt cgcgtgcagc gcgaccagct cgccgcgcac ttcggaacgg
     6781 tgcgctgcgg caagtgcggc ggcgtgttca acggcgtcgc ggcgctggtg gaagagggcg
     6841 cggagaagct ctcgctcgac ccctcgccgc agctcggact gttcgacccc acgcggcggc
     6901 cggcgcaggc ggccggcatg cgggacgacg cgccgctgcc cgaattcctc gcgggggaac
     6961 gcgccgcccg gccgcggctc tggctgtggc tgctgctcag cctggcggcg gctgcgatgc
     7021 tcggcgcgca gctctacctt tcccgcaacg aggcgacgag ctggctcccg gaattgcgcc
     7081 ccgccttcac gctggtctgc cgtttggccg gttgctcggt cgcgcttcca cggcgcccgg
     7141 agctgttgaa gatcgtcgct tcggacatgc atgacgactc cgcgcgccag gtctttgtgc
     7201 tcaatgcgac gctgcgcaac ctcgcccggt ttccccagca gtatcccgat ctggagctca
     7261 cgctcacgaa cgcaggcaaa ccggtcaccc ggcgagtgct tggacccgag gagtacctcg
     7321 actcccgcgt cgcctcgcgc gtgatcagca ctgggcttga ggccggggcc gaggagccgc
     7381 tgcgggtgca cgtcgatgcc aagctcgtgc gcgcgaccgg atacgagctt tgcatctacc
     7441 cgcaggactg ccttaagcac gcgacaaaat gaaaaagatc ctcgtcaccg gctcgatcgc
     7501 ctacgacacc atcatggtgt tcccggatcg cttccgcaat catctgctcg ccgaccagct
     7561 acacatcctg aacgtctgct tcctcactcc cgagatgcga cgcgagtacg gcggcaccgc
     7621 cggcaatatc gcctacaacc tgaagctgct cggcgaggat gcgctggtga tggcgaccgt
     7681 gggggaggac attcagcctt atttgcagcg cttcgaagcg ctcggcatcg gcacgcgccc
     7741 cctgcgccgg atccccggcc agtttaccgc gcaggcgttc atcactaccg atctcgacga
     7801 caaccagatc accgccttcc atcccggcgc gatgaatcac tcgcaccaga accacgtgac
     7861 ccgcgagctc ggcgcgggcc tcgcgatcat cgctcccgac ggcaaggacg gcatgctgca
     7921 gcatgcgcgc gaatgcgcat ccgccggggt ttcgttcatg ttcgaccccg gccagggcct
     7981 gcccatgttc tccggcgacg agctcggcga gttcgtgcgg ctcgccgact acgtggcggt
     8041 gaacgactac gagggcaaga tgctggagga aaagaccggc cgcaagctag aagcgctggc
     8101 gcgcgaggtg aaggcgctgg tctgcacgct gggagccaag ggctcggtga tcctcgccca
     8161 gggccaacgc cacgaggtgc cgagcgcgcc cgcggacgcg gtggtggatc cgacgggctg
     8221 cggcgatgcc taccgcgccg gcctgctcta cggcatcgcg cacggctggg actggccgag
     8281 caccggcaag ctcggcgcag tcatgggcgc gctcaagatc gcccaccgcg gcgcgcagaa
     8341 ccacgttgcc acgcgagccg aaatcgaggg caggttccgc aaggcgttcg gctattcacc
     8401 gtggcaaggc tgacctgatt tttgcggcgt agtcctcgag cttctcgcgc ggcgggtttt
     8461 tcaccggcca ggtgagcgcc atgccgtcgc cttcgtggcg cggcaccagg tgcacgtggt
     8521 agtggaacac cgtttggccg gccgccttgc cgttcgcctg atagaccgaa agaccgtgcg
     8581 gggcaaacgc gtcacggatg gcacggccta cctttgccgc aacccgcaat accgcccccg
     8641 cctgtgcgtc gttcagcgca taaaggttct ccgcatgcgc cttcaccgta accaggacat
     8701 gccccggatt cacctggcct aggtccatga aggcgagggt gtgctcgtcc tcgtgcacca
     8761 cggtggccgg gatctgccgc gccacgatcc tgcagaacac gcaatcgctc atgcttcgcc
     8821 tcctggagac tgatagccgg ggcaagcggc ctgatagagg gtgcggtcct cgagccgcag
     8881 cgcgctgagc gcgccgcccc agatgcagcc gctgtcgagc ccgatcgtac ccggctcgag
     8941 caccagcccg agctgcgacc aatggccgaa gatcagcgtc ttgccgcgct cgcgcttgcg
     9001 cgtctcatac cagcgccgat agccccgggc cggctcgatg ccggcgcggc tgaggtccat
     9061 ccgccccgcg cggtcgcagt agcgcatgcg agtcatcgcg ttgacaatca cgcgcagccg
     9121 gtcccagccc gaaagcgaat cccgccagcg cgacggcgtg ttgccgtaca tatgctcgag
     9181 aaacgtacgg tagtcgcgcg ccgcgagcgc ggcgctgact tctctgccga ggcgcttcgc
     9241 cttcgcgatg ctccactgcg gcaggaggcc ggcgtgcacc atggcgtagc cgccttcggc
     9301 gtgcaccatc ggctgccggc gcagccacgc gaggagctcg gccgcgtcgg gcgcgtcgag
     9361 cacgtcctgg aaagtgtcgg cgcggcggat cttctcgata ccctcgtatt gcgcgaccag
     9421 atgcaggtcg tggttgccga gcacgactac cgcgcgctcg ccgaggtctc tgacgaaccg
     9481 cagcacctcg agcgacttcg gcccgcgatt gacgagatcg cccacgaacc aggcgcgatc
     9541 ctgcttcttg aagccgactt tcgccaggag ttgccgcaat tcatcgaagc atccctgcac
     9601 gtcgccgatc gcgtaggtcg ccatcggcgc cgactatagc tcagccgact aaaagcttgc
     9661 gtcgagccgg cggtactgga tcgcctccgc gatgtgctcg ccgctgattg aagttttctg
     9721 cgccagatcc gcaatcgtgc gcgcgacgcg caggacccgg tgataagcgc gcgccgagag
     9781 cagcaagcgg gcgagcgcat ggcgcaatag ctggtctccc tcgcggtcgg tggcgcaatg
     9841 ccggtcgatc tcgcgcgtgc cgagcaacgc attcggcttg ccttgtctcg acgcctggag
     9901 cttgcgcgca ctctcgacgc ggccgcgaat cgcctccgag gcctcgcccg acaccggcgc
     9961 caccagctcg ctctcgcgcg gcggcggcac ctcgagcttg atgtcgatgc ggtcggcgag
    10021 cggcccggag atgcggccgc ggtagcgcgc gatgcgctcc ggagtgcagc ggcagcgccc
    10081 gctgcgatcg ccgcaatgac cgcacgggca ggggttcatc gcgccgatga gctggaagcg
    10141 cgccgggaac tgcgcctggc gtgcggcgcg cgcaatggaa acgcgtcccg actccaacgg
    10201 ctcgcgcagc gcctcgagca cgtcgcggtc gaattccggc agctcgtcga ggaacagcac
    10261 gccgtgatgc gcgagcgaga tctcgcccgg ccgcggcagc acgccgccgc cgaccagcgc
    10321 cgcggccgag gcactgtggt gcggcgcgcg ataggggcgc tcgccccagc gcgccgcttc
    10381 gaagccgctc gcggaaaccg agtggatcgc ggcgacttcc agcgcttcgg cttcggaaag
    10441 cgcgggcagg atcccgggga agcgcgcggc cagcatcgac ttgccggtgc ccggcgggcc
    10501 gatcatcaag acactgtgag cgcccgccgc ggcgacttcc agcgcgcgtt ttgcctgctg
    10561 ctgccccttg acgtcgctga agtccgcata cgccacgctc gcgcagcgcg cctcggcgcc
    10621 gtactgctcg aggggcttct cgccggcgag atgcgacacc acttcgagca gcgtacgcgc
    10681 cggcaggatg cgcgcaccct cggcgagggc ggcctgcggc gcgttgcctt ccggaagaac
    10741 aaacgcgcgg ccggcggcgc gcgccgaaag cgccatggcg agcgcgccgc gcaccccgcg
    10801 cagctctccc gtaagcgata gctcgcccgc gaattcctgc ccggcgagcg cctgcggcgc
    10861 gagctggccg ctcgccgcga gaattccgag cgcgatcggc aggtcgaagc ggctggagtc
    10921 cttcggcagc tcggccggcg cgaggttgac ggtgatgcgc cgcgcgggaa attcgaagtg
    10981 aacgtggttg agcgccgcgc ggacgcggtc gcgggcttcg cgcacttggg cgtcgggcaa
    11041 accgacaata tggaacgccg gtagcccggg gcccaggtgc acttcgaccg tgacctcggg
    11101 cgcgtctatg cctgcaagcg cgcggctcgc gaccgtagcg accgtcatgg ttcccctttc
    11161 tctggggaat gaacgctcga gacgggcttg ccgtttaccg cgacttgcga gtctccagct
    11221 ccgcgattcg cgcttccagc tcggtcagct tcgcctgcgc gcgctcgagg agcttcttct
    11281 gcacctcgaa gtcctcgcgc agcacgaggt cgaggcggtc gaaccacgcg gccagcagcg
    11341 cgcgcaggtt tttctcgatg tcctgcgccg gcgagccgcg cagcgcttcg tcgatgcggg
    11401 cgcgcaggtc gtcgaagagc ttgggatcga tcatggcgca aaggtaccgc accacgcccg
    11461 ggcgctgtcc tcgaaagcgc gcaccaaaat agggctcagc ttggccgaac caaaagtaac
    11521 ccattgagta caaaaacttt ttttgatggc acaggagatg ctaataggcg acggcactac
    11581 ctatttcaac tcaagggagc ttcccccaat gcgcaagtcc atcctcacct tgtccgtcgc
    11641 tgccgcgctc gccgcccccg gcctggtcgc cgcgcaaact gcgccagcgc ctgcagccgc
    11701 cccgtcgccg gtgaccggca acatggcaat cgtcagcgat taccgattcc gcggcatctc
    11761 ccagaccttc gaattgccgg ctatccaggg cggcatcgac tacgcgcacc agagcggcat
    11821 ctacctcggc aactggaatt cgagcgtgag cggactgagc ttcaccgata ccgccggcat
    11881 cgagatggac ttctacggcg gctggaagaa gagcttcggc gacatcggct tcgatatcgg
    11941 tttgctgcag tactactacc cgggcgccaa gaccggggga ggcgaagagt acaacacgct
    12001 cgaaggctac atcggcgcgt cgtggaagtg gctgacgctc aaatactcgt acacgttcag
    12061 cgactacttc ggcctggtga actccgacgg gtcgggctac ctcgatctcg gcttcaccat
    12121 ggaggtcgcg cccaagctca ccatcgtggc ccactacggc atgcagacga tccagaacta
    12181 cggcgcgctg gattacaacg actggaagct cggcgtcacg tatgacctcg ccggctggat
    12241 cctgggcgcc gcctatatcg acaccgacgc cgacagcggc ttgtacacgc tgacgaactc
    12301 gaagggcaag tccaaggacc tcggcggcag cacggtggtg ctctcgatca tgaaaacatt
    12361 ctgaggaccc catgaaactc gttaccgcaa tcatcaagcc gttcaagctc gacgaggtgc
    12421 gagaagcgct ctcggcgatc ggcgtgcagg gcatcaccgt caccgaggtc aagggcttcg
    12481 gccgccagaa aggccacacc gagctctacc gcggcgcgga gtacgtggtc gatttcctgc
    12541 ccaaggtgaa ggtcgaggcc gcggtcaagt ccgacctgct cgagcaggcg ctcgaggcga
    12601 tcgagagatc ggcgaacacc ggcaagatcg gtgacggcaa gatcttcgtg ttcgatctgg
    12661 aaaaagtcat ccgcatccgc accggcgaga ccgatgcggc ggcgctctga ggagaacgtg
    12721 atgaagaaat tctgcgcatt cctggcgctg tggctgtcgc tcgccttcgc gggcgcgacg
    12781 ctggcgcaag acaagcccgc ggccacgccg gacaagccgg ccgcggcgga agtgaagaag
    12841 gaggaggcga agcccgcgcc gccgacgccg aacaagggcg acgtcacctg gatgctgatg
    12901 tccacggcgc tcgtcatcat gatgagcgtg ccggccctcg cgctgttcta cggcggcatg
    12961 gtgcgctcga agaatgcgct ctcggtgctg atgcaggtgt tcgtggtgtt ctcgctggtg
    13021 acggtgctgt ggtgcatcta cggctacagc ctcgccttca cggagggcaa cgcgttcttc
    13081 ggcagctgga aggatcgcct gttcctgagc ggcgcgctcg atgccggaaa gggcgagttc
    13141 gccaacgcgg ccaccttctc caagggcgtg gtgatcccgg agatcgtgtt cgtcgcgttc
    13201 caggccacgt tcgccgccat cacctgctgc ctgatcctcg gcgcctttgc cgagcgcgcc
    13261 aagttcgcgg gggtgcttgc gttcatcgtc ctgtggttca ccttcgccta cacgccggtc
    13321 gcgcacatgg tgtggttctg gatgggaccc gacgcgtaca ccagcgccaa ggtggtggac
    13381 gagatgaacg ccaaggcggg ctggctgtgg cagatgggcg cgctcgactt cgccggcggc
    13441 acggtggtgc acatcaatgc cggcgtggcg gggctggtcg gcgcctacct cgtcggccgg
    13501 cgcatcggct acggcaagga agcgatgccg gcgcacaacc tgccgctcac gatgatcggc
    13561 gcctcgctcc tgtgggtggg ctggttcggc ttcaacgccg gctcggcgct cgaggccggc
    13621 aactcggcgg cgctcgcttt cctcaacacc tacctcgcca ccgcctgcgc cgtgctctcg
    13681 tggagcctcg gtgaagcgat gttcaagggc aagccgacga tgctcggcgc cgcctccggc
    13741 gcggtagcag gcctcgtggc gatcactccg gcggcaggca acgtcgggat tccgggcgcg
    13801 ttcgtcatcg ggctctcggc cggtctggtg tgcctctggg gtgtgagcgg cttgaagaag
    13861 ctgctcggcg ccgacgactc gctcgacgtg ttcggcgtgc acgcggtcgg cggcattctg
    13921 ggcgcccttc tcaccggggt gttcaactcg ccgggcctcg gcgggccggg gttcgtgaac
    13981 aactggttcg aaatgaagca gggcttcgag agcatcggtg gccagctctg gatccaggcg
    14041 aaagccgtcg gcgtgacgat cctctggtcg ggcatcgtcg ccctggtcgc ctacaagctg
    14101 gtcgactgga cgatcgggct gcgcgtcgcc gaagaggaag agcgcgaagg cctggacacc
    14161 gcctcgcatg gggaggccgc ctaccgcatg taggagggct gctgctgcaa ccggaaaagg
    14221 gcgctccgcg ccctttttct tttgcgctac ccggcgagct tctcgagaag ggcgagcaat
    14281 tgcgtgcgct ccgccgcggt cagccgcgaa gcgacccggc gctcgtgcac gcggatgcgg
    14341 cgcttgaggc tcgccaccag ggcgcgcccg gcgcgcgtga gccacaggcc gttggtgcgc
    14401 cggtcttcgc cgcggcggcg ctcgaccagc tcgcgcgctt cgagcgcatg caggacccca
    14461 accatggttg aacggtcgag ccccaccgcc tgcgcaaggc ggctctgcgt caccccgggg
    14521 ttcgcctcga tcaggaacag gatcccggcg cggccgggcg acgcctcggg cacggcggcg
    14581 gcgtagtcgc ggaacaccgc ctgctgcgcg aggcgcaggc ggtagccgag gaggccggga
    14641 aggggaccgg gcttcagcat gacagatgag tttgtttggg ttccaaacaa ttggtaccat
    14701 cgtaagttct accggcggcg catgcaaatc acactcgttg gccacgacca gcccgtaccg
    14761 gtgcaggcgg gcgatacggt gctcgcgtcc ctgctccgcg ccggcctcgc attccccttc
    14821 tcctgccagg cgggcaactg cggcacctgc aagtgcgagc tggtgtccgg cgacgtgcac
    14881 gagctcgagt actccgagca tgcgctttcc gccgccgagc gcgctcgcgg catcgtgctc
    14941 gcctgccgca cccaggtctg ggacgacacc gtcgtgcgcc gcatcgacgc cgaggagctg
    15001 gtggtccatc cgtcgcgcgt catgcgctgc cgcgtgctcg aggtcgagag cctgacgcac
    15061 gacatcaagg ggctgcggct cgcgatcgag gcgggcggcc cgtttacttt ctctgccggg
    15121 caatacgcgc aggtcgagtt cgcgcccggg ctatcgcgcc actactcgat ggcgaacacc
    15181 cccgacgagc cggagctcct gttccaggtg cgccacatgc cgggcgggcg caccagcagc
    15241 tacgtcgcga cgcagctcaa ggtgggcgac cggatcaagg tgtcgggccc gctcggcatc
    15301 gcctatttgc gcgacgcaca ccgcgggccg gtgctgctcg tcgccggcgg cagcggcctc
    15361 gcgccgatcc agtcgatcct ctgcacgctg ctcgagcgcg ggcacgagtc gcccgcgacg
    15421 ctctacttcg gcgtgcgcag cgagcgcgac ctttatcacg agccgctcct caggaacctt
    15481 gccgcccggc atccgaattt cagctaccac gtcgtccttt cggagcagaa gggcgcgagc
    15541 gggcgccgct atggcctcgt ccatgaagcg atcgagatcg gggacgccga aaacgccatg
    15601 gcctaccttg ctggcccgcc ggtgatggtc gaagccgcca ccgcgtggct tgccgcgcgc
    15661 ggcctggcgc agcggcagat tcacgccgac gcgttctaca accagaatta ggcgatggac
    15721 ctccaaggca agggtgcaat cgtgaccggc ggggcgcgcg gcatcggcct cgcgatcgtg
    15781 gaagagctcg tcgcgcaagg cgcctcggtg gtcatcgtcg attccggcgt ctcgatctcc
    15841 ggggagcctc tggagcctga gattgccaag caggtcgcgc agcgcattgc cggttctgtt
    15901 gccgtctgtg gcgacatcgc cgacccggac gtggcccggg aagcggtgga cctggcctgc
    15961 cgcaagttca agtccgtgga catcgtggtc aacaacgccg cgatcatccg cgacgcgttc
    16021 atcttcaagc ccgagcccga tgactgggac gcggtgatcc gcaccaacct gagcggcgcc
    16081 cagcgcctgc tcggcgcggc gacgccggtg atgcgcgagc aggtgaagaa cggccgcgcc
    16141 ccgggagcga tcgtgaatat cgtctcgacg gcgggcatct acgggaactt cggtcagggt
    16201 gcgtacggcg cggccaaggc aggcctcatc ggcctgaccc gcgtggtggc gcacgacctg
    16261 gcacgctcga agatccggtg caacgcgatc gcgccgttcg cggcgacgcg cgtgaccgaa
    16321 agcatcaagc ccgcgaacga ggctcaggcc gcttacaagg cgaaggcgct gcgcgtgccg
    16381 gcgcgccacg tggcgcggtt ggtgagcctg ctcgccagcg accgcgcagc gcacgtgacc
    16441 ggccagctct tcggcgtgcg cgggcgcgag gtcttcgtgt tctcccagcc gcggccggcg
    16501 cagcgcatgc tgcagaccga ccagggcttc gacctgaagg cgctcgaacc ccacttcacc
    16561 gcgctcgtca ccgacctcga agccttctcc ggcgaccccg ttctctgagg cagcgaacat
    16621 gtccgcgtat cccagcatca agaccgtcga agagttccgg cagcagcccg aggcgtatca
    16681 gacggcggtg cgcaagatcg tgcgcagcca cgcgctgaac gagctctacg gcgcgcaggt
    16741 cttcgacgag cccgccatcg gcctcgcgcc gacgccgtac gccaaatggc tgacctgccg
    16801 cgtggcgatg gaggagtatc accaccacgt gcgatttcgc gagctcgccg accagatcgg
    16861 cgttcgcgcg gaggaaatgg acgcgcgcaa caaacgcccg ctcaacatct tcgaggtgcc
    16921 cctcgagagc tgggagcagt tctgcgtcat caagatgctc ggcgacctcg ccgagatcct
    16981 ccaggtcgag gacctgctgc actgctcctt cgtgccgctg cgcaacattg cgcgcaccac
    17041 gatgccggag gagaagttcc acgcccagtt cggcctcgat ttctgcgtcg a
//
LOCUS       DATBSZ010000031        12323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1096313, whole genome shotgun sequence.
ACCESSION   DATBSZ010000031 DATBSZ010000000
VERSION     DATBSZ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12323
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096313"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VIG70_01895"
     CDS             complement(<1..343)
                     /locus_tag="VIG70_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8249330.1"
                     /translation="MDIHIRELSRLDRAALEPHFLALDSEDRRLRFGVQINDDAVRAY
                     VARIDFERDAAFGVYDDGLHLVGTAHVARSGGHAELGVSVLPGHRGRGIGGALLARAH
                     LRARNWGVWALF"
     gene            complement(395..1666)
                     /gene="hemL"
                     /locus_tag="VIG70_01900"
     CDS             complement(395..1666)
                     /gene="hemL"
                     /locus_tag="VIG70_01900"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY8249331.1"
                     /translation="MKKSRQLFERAQARIPGGVNSPVRAFRAVGGSPVFFERASGPYL
                     WDADGTRYIDYVGSWGPMLAGHGHPAVVEAVQEAASRALSFGAPTEAEVELAELLCRL
                     LPSLELVRLVSSGTEATMTALRLARGFTGRSLVVKFEGCYHGHADALLVKAGSGALTF
                     GNPSSAGVPPETAAATIVLDYNDIDQVRTLFSERGKAVACVIVEPVAGNMNLVLPATG
                     FLETLREECTRHGAVLIFDEVMTGFRVALGGAQQRFGIRPDLTTLGKVAGGGLPLAAV
                     GGRREIMEKLAPLGPVYQAGTLSGNPIAVAAGLATLKVVSVNGFCEKVEATTKALVQG
                     LVAAGGKGFSAQSIGSMFGVYFRDSPPKSFAEVMQCDRERFNRFFHEMLTRGVYLAPS
                     AYETGFVSAAHGVGEIEATFGAARAAFAKIS"
     gene            complement(1663..2262)
                     /gene="thiE"
                     /locus_tag="VIG70_01905"
     CDS             complement(1663..2262)
                     /gene="thiE"
                     /locus_tag="VIG70_01905"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY8249332.1"
                     /translation="MMRGLYAVTPDTADTDVLLRKVEEALKAGVAMLQYRNKIISKDK
                     RLLQAKDLAPLARGYGVPLIVNDDVEIALTVGANGVHLGKEDGDLAGARAKLAGRILG
                     ASCYDDLEKARAAVRAGADYVAFGAVFPSPTKPGAARAPLSLFGSGLGVPLCAIGGIT
                     LKNAPALIAAGASLLAVISDLFDAPDIGARAGEYRKLFQ"
     gene            complement(2259..3008)
                     /locus_tag="VIG70_01910"
     CDS             complement(2259..3008)
                     /locus_tag="VIG70_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEY8249333.1"
                     /translation="MPPIVLTFAASDPTGGAGLQADLLTLAALGCHPLSVLTGLTVQD
                     TSGVEHLESIAADLVTRQASRVLAETRVAAFKVGVLGSAENVRAVAAIAAAHARVPLV
                     LDPVLASGRGDPLANEAVLKALLELLVPRATVVTPNTVEAAQLGGAKRLLELGCRYVL
                     VTGTHEPGAEVSNKLYDSSGLVREDRWQRLPGSYHGSGCTLASAIAAQLAKGRDVPEA
                     ARRAQKFTWQALAAGFRSGAGQSLPNRFFGK"
     gene            3046..3216
                     /locus_tag="VIG70_01915"
     CDS             3046..3216
                     /locus_tag="VIG70_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEY8249334.1"
                     /translation="MQFKTWMCLICGFVYDEAAGLPDEGIVPGTRWEDIPINWTCPEC
                     GARKEDFEMVEI"
     gene            3276..3848
                     /locus_tag="VIG70_01920"
     CDS             3276..3848
                     /locus_tag="VIG70_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY8249335.1"
                     /translation="MSARMSTVNLTHHFLIAMPNMADPYFAKTLTYICEHNDRGALGL
                     VVNRPLDMTLQALFERLSLTLRDRERRDAPVYFGGPVQTDRGFVLHAPAGNWQSTLRV
                     GDAIGLTTSKDILEAVGRGEGPARMLVTLGYAGWSPGQIEHELSQNAWLTVEARDGII
                     FETPAEERLPAAMELLGLDFARLQDEAGHA"
     gene            3841..4227
                     /gene="ruvX"
                     /locus_tag="VIG70_01925"
     CDS             3841..4227
                     /gene="ruvX"
                     /locus_tag="VIG70_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY8249336.1"
                     /translation="MPSRGTVLAFDFGLRRIGVAVGEPELRTAHPLPAISAFAQIQKL
                     VEEWKPASLVVGLPTSAQGEPHRMTRQAEDFARRLERRFKLPVARVDERYTSVEAEHR
                     LRGVKKKAIDSVAAQLILEQYFDQAA"
     gene            4211..4717
                     /gene="pyrR"
                     /locus_tag="VIG70_01930"
     CDS             4211..4717
                     /gene="pyrR"
                     /locus_tag="VIG70_01930"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY8249337.1"
                     /translation="MTRLPDAEKLCEQLVAELKPRIGPKSAMVGLYTGGAWLAERLHP
                     MLDMKSPLGLMDIAFYRDDYAARGLKHDPKRTKIPFDVNGAELLLVDDVLYSGRTVRA
                     AMNELFDYGRPASISLVVLVDRGGRQLPICAQHVGARVEVPGGMRLRLKQEAGGKLAL
                     VLEPERAA"
     gene            4701..5657
                     /locus_tag="VIG70_01935"
     CDS             4701..5657
                     /locus_tag="VIG70_01935"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY8249338.1"
                     /translation="MSVQRNPQLNEKGELQHLLSTEGLPREILLQILDTAESFVGVTE
                     REVKKVPLLRGKSVFNLFFEPSTRTRTTFEIAAKRLSADVINLNITVSSATKGESLLD
                     TVANLSAMQADIFIVRHEFSGAPFLIARHVKPHEHVINAGDGRHAHPTQGLLDLFTIR
                     HYKKDFTGLTVAIVGDILHSRVARSLIHGLTTLGVPEVRVIGPQTLIPAGVEALGVRV
                     FHDMREGLKDCDVVVMLRLQNERMRGALLPSAQEFNKHYGLSGDKLGVARPDAIVMHP
                     GPMNRGVEIDSTVADGPHSVILPQVTFGIAVRMAVMSIVAGN"
     gene            5657..6916
                     /locus_tag="VIG70_01940"
     CDS             5657..6916
                     /locus_tag="VIG70_01940"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY8249339.1"
                     /translation="MRLHIKNGRIVDPASGKEGAGDVFMVDGKISPEGGKADRVIDAK
                     GLVVAPGFIDLSARLREPGYEYKATLESEMDAAVAGGVTSLACPPDTDPPLDEPGLVD
                     MLRRRAKALSRARVYPVGALTVKLEGQALTEMGQLTDAGCVAFSQANVPMADTQVLWR
                     ALQYAATFGYPVWLRAEDASLAKGGVSHDGEVATRLGLPGIPSFAETIALATLLELVR
                     ATRARVHVCRLSTAGGVELMRRAKQEGLPVTCDVAVHHVHLCDMDLGYFDSQCRLEPP
                     LRSQRDRDALSRGLAEGVVDCICSDHTPVDEDGKHLPFAQAEPGATGLELLLPLTLRW
                     GQANKLGLAATLARITSEPARILGVASGKLAPGAPADVVLFDPAAPFKVAPETLKSQG
                     KNTPFLGYELAGRVRTTIVAGNVVYEQ"
     gene            6913..7380
                     /locus_tag="VIG70_01945"
                     /pseudo
     CDS             6913..7380
                     /locus_tag="VIG70_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018127.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
     gene            7475..8599
                     /locus_tag="VIG70_01950"
     CDS             7475..8599
                     /locus_tag="VIG70_01950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY8249340.1"
                     /translation="MRSSLALLVVLFSLTATAAAPQPPSVIGKSWMIGDLSSGQVIAA
                     QKADERFEPASLTKLMTAYVVFAALRDRKLAPDQQVNVSTKAWKAAGSRMFIEPRKPV
                     TVDELIRGMVVQSGNDACIALAEAVAGNEEVFVQMMNREAARLGMKSSKFMNASGLPD
                     AQHYSTAQDLYTLAAALIRDFPNEYAQYYSQKEYRYNNITQPNRNRLLWIDPSVDGMK
                     TGHTEAAGYCLVASSRRGGRRLLSVLLGSTSEATRAQESLKLLNWGYQFFDSVKLHAA
                     GNPVRSLEVWKGAARNVKVGVGNDLFVTVPKGEADRLKAELVSQQPLVAPLGKGQQVG
                     VVRVTHDGKPFGEYPLITLEPVAAAGFFGRTWDTLRLWLK"
     gene            8587..9468
                     /locus_tag="VIG70_01955"
     CDS             8587..9468
                     /locus_tag="VIG70_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HEY8249341.1"
                     /translation="MAEMTVFLNGKLLPIEQATVSVLDRGFIFGDGVYELVPVYSRVP
                     FRLEEHLVRLERSLGEAKIRNPYSRQQWRAHVYQLADAQPFEDQGIYFQVTRGVAKRD
                     HAFPKSVEPTVFMMANPLTNPPQAQIENGSAAVSAQDNRWLRCDIKSISLIGNVLLRQ
                     LSAEAGAAETILFRDGKLTEASASNVFIVKHGVIQSPPKTNLILPGITYDVVVELARA
                     SSLAFEFRDIPEAEVRAADEVWVTSSSKEVLAIVELDGKRVGDGRPGPVFRRMYQLYQ
                     DFKQKVMRAGKREAVSA"
     gene            9482..9754
                     /locus_tag="VIG70_01960"
     CDS             9482..9754
                     /locus_tag="VIG70_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEY8249342.1"
                     /translation="MPVVADNSPLAFPCEFPIKVMGRKQPRFVQAVTSIVRKHAPDFD
                     AATVEMRPSRQGRYLSVTCTVRATSREQLDALYLELCDHPDVVMVL"
     gene            9758..10369
                     /gene="lipB"
                     /locus_tag="VIG70_01965"
     CDS             9758..10369
                     /gene="lipB"
                     /locus_tag="VIG70_01965"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY8249343.1"
                     /translation="MASTLLEEIVVKRLGRAEYSSAWEEMRAFTRARDESTPDELWIL
                     EHPPVYTVGQGASLPQTGNAIPVVKTDRGGDITYHGPGQAVIYTLVDLARRAIKVKRF
                     VWLLEQAVIDLLGGRAERKPGAPGVYVDGAKIAALGIRVVRGRAYHGLALNVDMDLAP
                     FAAIDPCGYPGLRVTQMKELGFADSPETAASRLSQILVEKLDA"
     gene            10362..11300
                     /gene="lipA"
                     /locus_tag="VIG70_01970"
     CDS             10362..11300
                     /gene="lipA"
                     /locus_tag="VIG70_01970"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY8249344.1"
                     /translation="MREAGVKERGARKTIRIVRQENATTRLPKPAWIRVRAASPNSRF
                     YDIKRILREQKLHTVCEEASCPNIAECFGKGTATFMILGDICTRRCPFCDVAHGRPLA
                     PDAEEPRHLAETISLLGLSYVVITSVDRDDLRDGGAAHFVACIAETRKVSPQTTIEVL
                     VPDFRGRLERALEVLDAAPPDVMNHNLETVPRLYRKARPGGDYAHSLRLLQEFKQRHP
                     AVPTKSGLMVGLGETDEEILAVMRDLRAHDVDMLTIGQYLQPSPHHLPVERYVDPGIF
                     KEFENQALAMGFRHAAVGALVRSSYHADQQAHEAGI"
     gene            complement(11297..11806)
                     /locus_tag="VIG70_01975"
     CDS             complement(11297..11806)
                     /locus_tag="VIG70_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8249345.1"
                     /translation="MKAKLIVGIGALASAVGWAAAIVVLSAHTLLKPAPEVRFHPLSG
                     ATFSTSDLRGKVVLVNFWGSYCTPCVKEMPKIVAAHEKFASRGYETVAVAVRHDNPHR
                     VAAFAAKRGLPFRITLDSSGEAAKEFGNVRITPTSFLIDRQGRVLRRYVGEHDWVALH
                     EIVERELAR"
     gene            11805..>12323
                     /locus_tag="VIG70_01980"
     CDS             11805..>12323
                     /locus_tag="VIG70_01980"
                     /inference="COORDINATES: protein motif:HMM:NF025221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEY8249346.1"
                     /translation="MRTAIFAALVMTAAAPALGQLERLTQRDALAGLKAALERGSRAA
                     VAELGRTDGFFGNPQVKIPIPESLERTEKLARRLGFSNEVEELVEAMNRAAEAAVPEA
                     RKLLLDSVKKMTVQDAKGILTGGDTAGTAYFRRTADKTLHDRFLPIVSTATEKVGVAQ
                     RYKEFARPAAALG"
BASE COUNT         2096 a         4095 c         4164 g         1968 t
ORIGIN      
        1 tgaagagcgc ccacacgccc caattgcggg cgcgcaggtg cgcgcgcgcg agcagtgcac
       61 cgccgatgcc gcggccgcgg tgccccggca gcacagagac cccgagctcg gcgtgcccgc
      121 cgctgcgggc gacgtgcgct gtaccgacca ggtgcaggcc gtcgtcgtag acgccgaagg
      181 ccgcgtcgcg ctcgaaatcg atgcgcgcga cgtatgcgcg caccgcgtcg tcgttgatct
      241 gcacgccgaa gcgcagccgg cgatcttcgc tgtcgagggc gaggaaatgc ggctcgagcg
      301 cagcgcgatc gagccgggag agctccctga tatggatgtc catgtcggct tgctgttaga
      361 cccagcacgc cacgcaaggt ttaagcgtgt tttgctacga gatcttggcg aaagcagcac
      421 gcgcggcgcc gaacgtcgct tcgatttcgc ctacgccgtg cgccgcggac acgaatcctg
      481 tctcgtaggc cgaaggcgca aggtacacgc cgcgcgttaa catctcgtga aagaagcggt
      541 taaagcgttc gcggtcgcat tgcatcactt cggcgaagct cttcggcggg gagtcgcgaa
      601 aatagacgcc gaacatgctg ccgatcgact gcgccgagaa gccttttcct cccgccgcca
      661 cgaggccctg gaccagcgct ttcgtcgtgg cttcgacttt ttcgcaaaac ccgttcaccg
      721 atacgacctt cagggtggct agtccggcgg cgacggcaat tggattgccg gaaagcgtgc
      781 ccgcctgata cacgggcccc agcggcgcga gcttctccat gatctcgcgc cggccgccga
      841 ccgcggcgag cggcagcccg ccgccggcga ccttgccgag cgtagtgagg tcggggcgga
      901 tgccgaagcg ctgctgcgcc ccgccgagcg cgacgcgaaa gccggtcatc acttcgtcga
      961 agatcagcac ggcgccgtgg cgcgtgcact cctcgcgcag cgtttcgaga aatccggtgg
     1021 cgggcaagac caggttcatg ttgccggcga ccggttcgac gatgacgcaa gcaactgctt
     1081 ttccacgctc cgaaaaaagc gtcctcacct gatcgatgtc gttgtagtcc agcacgattg
     1141 ttgcggcagc agtctcgggc ggcacgcccg cggagctggg gttgccgaag gtgagggcgc
     1201 cggacccggc tttgacgagc agcgcgtcgg cgtggccgtg atagcagccc tcgaacttga
     1261 ccaccaggct tcggccggtg aagccgcgcg cgagccgcag cgcggtcatg gttgcttccg
     1321 tgccggagga gacgaggcgg acgagctcga gcgaggggag gaggcggcag aggagctcgg
     1381 cgagctcgac ctcggcctcg gtcggcgcgc cgaacgagag cgcgcgcgag gccgcttcct
     1441 gtaccgcctc gaccaccgcg ggatgtccgt gtccggcgag catcgggccc caggagccga
     1501 cgtagtcgat gtagcgcgtg ccatcggcgt cccatagata gggacccgag gcgcgctcga
     1561 agaagaccgg ggagccgccg acggcgcgaa aggcgcgcac cggcgagttg acgccgccgg
     1621 ggatgcgggc ctgggcgcgc tcgaagagtt gcctgctttt tttcattgga agagcttgcg
     1681 gtattcgcct gcccgcgcgc cgatgtccgg cgcatcgaag agatcggaga tcaccgcgag
     1741 caggcttgct ccggcggcga tcagcgcggg cgcgttcttc agtgtaatgc cgccgatcgc
     1801 gcacagcggc acgccaaggc cgcttccgaa cagggagagc ggcgcgcgtg ccgcgccggg
     1861 cttggtcggg gaggggaaaa cggctccgaa ggcgacgtag tcggcgccgg cccgcacggc
     1921 ggccctggcc ttttccaggt cgtcgtagca ggaggcgccg agaatccttc cggcgagctt
     1981 ggcgcgcgcg cccgccaggt cgccatcctc ctttcccaag tggacgccgt tcgcgccgac
     2041 ggtgagcgcg atctcgacgt cgtcgttcac gatgaggggc acgccgtaac cgcgagcgag
     2101 cggcgccaga tctttcgcct gaagcaggcg cttgtccttg gagatgatct tgttgcggta
     2161 ttgcagcatg gcgacgccgg ctttcagcgc ctcctcgacc ttgcgcagga ggacgtccgt
     2221 gtcggcggtg tcgggcgtga cggcgtagag gccccgcatc attttccgaa gaagcgattg
     2281 ggcagcgact gcccggcgcc gctgcgaaat cccgcggcga gcgcctgcca ggtgaatttc
     2341 tgcgcccggc gcgcggcctc gggcacgtcg cggcccttcg cgagctgcgc ggcgatggcg
     2401 gaagcgaggg tgcatcccga gccgtggtaa ctgcccggca gccgctgcca tcggtcctcg
     2461 cgcaccaggc cgctcgagtc gtagagcttg ttgctcacct cggcgcccgg ttcgtgcgtg
     2521 ccggtgacca ggacgtagcg gcagccgagc tcgagcaggc gcttcgcgcc gccgagctgc
     2581 gcggcctcca cggtgttcgg cgtgaccacg gtcgcccggg gcacgagcaa ttccagcaat
     2641 gccttcagga ccgcttcatt cgccagggga tcgccgcggc ctgaagcgag caccgggtcg
     2701 agcaccagcg gaacccgggc gtgcgcggcg gcgatcgccg ccacggcgcg cacgttctcg
     2761 gcggagccga ggacgccgac cttgaacgca gcgaccctgg tttcggcgag cacgcggctc
     2821 gcttggcgcg tgaccagatc ggcagcgatg gattccaggt gctcgacgcc gctcgtgtcc
     2881 tgcaccgtga gcccggtgag caccgaaagc ggatggcagc cgagcgcggc aagcgtaagc
     2941 aggtcggcct gcaatccggc gccgccggtc ggatccgacg ccgcgaaggt aagaactatc
     3001 ggtggcatga gttaatatcc ggcccatttt atgaccgaga gccaagtgca gttcaagacg
     3061 tggatgtgcc tcatctgcgg cttcgtctac gacgaagcgg cggggctgcc cgacgagggc
     3121 atcgtgcccg gcacgcgctg ggaagacatt cccatcaact ggacctgccc ggaatgcggc
     3181 gcacgcaagg aagatttcga gatggtggaa atctagaaaa acttccggca ggccggcgcc
     3241 tctaagccca ttacactaga ataaggggtg ggcgaatgtc cgcgcggatg tccacagtca
     3301 acctgacgca tcacttcctg atcgccatgc ccaacatggc cgatccgtac ttcgccaaga
     3361 ccctcactta tatctgcgag cacaacgacc ggggcgcgct cggcctggtg gtcaaccggc
     3421 cgctcgacat gacgctgcag gcgctgttcg agcgcctgtc gctcacgctg cgcgaccgcg
     3481 agcgcaggga cgcgccggtc tacttcggcg gcccggtgca gaccgaccgc ggcttcgtgc
     3541 tgcacgcgcc ggcgggcaac tggcagtcca cgctgcgcgt gggcgacgcc atcggcctca
     3601 ccacctcgaa ggacatcctc gaggccgtcg ggcgcggcga aggcccggcg cgcatgctgg
     3661 taacgctcgg ctatgccggc tggtcgcccg gccagatcga gcacgagctg tcgcagaacg
     3721 cctggctcac ggtggaagcc cgcgacggca tcatcttcga gacgccggcc gaggagcgcc
     3781 tgcccgcggc gatggagctc ctcggcctcg acttcgcgag gctgcaggac gaagcgggac
     3841 atgcctagcc gaggcacggt cctcgcattc gacttcggtc taaggcgtat tggcgtggcg
     3901 gtcggagagc ccgagctgcg cacggcgcat cctctgcctg cgatttccgc ttttgcgcag
     3961 atccagaaac tggtcgaaga gtggaagccg gcaagcctgg tcgtcggatt gcccacatcc
     4021 gcacagggcg aaccgcacag gatgacgcgc caggccgagg acttcgcgcg gcgtctcgag
     4081 cgccggttca agttgccggt ggcgcgggtc gacgagaggt atacgtcggt ggaagccgaa
     4141 catcgcctgc gtggcgtaaa gaaaaaggcc atcgactcgg tcgcggcgca actcatcctg
     4201 gagcaatact ttgaccaggc tgcctgacgc agaaaaactc tgcgagcagc tcgtcgccga
     4261 gctcaagccg cgcatcggtc cgaagagcgc gatggtgggc ctttacaccg gtggcgcatg
     4321 gctggccgag cgcctccacc cgatgctgga catgaaaagc ccgctggggc tgatggacat
     4381 cgcgttttac cgcgacgact acgcggcgcg cggcctcaag cacgacccca agcgaaccaa
     4441 gatcccgttc gacgtcaacg gcgccgagct gctgctggtc gacgacgtgc tctacagcgg
     4501 gcgcacggtg cgcgcggcga tgaacgagct gttcgactac ggccggcctg cgagcatctc
     4561 gctcgtggtg ctcgtggacc gcggcggacg ccagttgccg atctgcgctc agcatgtcgg
     4621 cgcgcgcgtg gaggtgcccg gcggaatgcg cctgcgcctc aagcaggaag ccgggggaaa
     4681 actcgcactg gtcctggagc ctgagcgtgc agcgtaatcc ccagctcaat gagaaagggg
     4741 agctgcagca tctgctttcc accgagggct tgccgcgcga aatcctgctg cagatactcg
     4801 ataccgcgga atcgttcgtc ggcgtcaccg agcgcgaggt gaagaaagtg cctctgttgc
     4861 gcggcaagag cgtgttcaac ctgttcttcg aaccctcgac gcgcacccgc accaccttcg
     4921 agatcgccgc caagcgcctc tcggccgacg tgatcaacct gaacatcacg gtgtcgtctg
     4981 cgacgaaggg cgaatcgctg ctcgacaccg tggcgaatct ctcggcgatg caggccgaca
     5041 tcttcatcgt gcgccacgag ttctccggcg caccgttcct gattgcgcgc cacgtcaagc
     5101 cgcacgagca cgtaatcaac gcgggcgacg gccggcacgc gcacccgacg caaggcctgc
     5161 tcgatctctt taccatccgt cattacaaga aggacttcac cggcctcacg gtcgccatcg
     5221 tcggcgacat cctgcactcg cgcgtggcgc gctcgctgat ccacggcctc accacgctcg
     5281 gcgtgccgga ggtgcgcgtg atcggcccgc agaccctgat cccggccggc gtcgaggcgc
     5341 tcggcgtgcg cgtgttccac gacatgcgcg agggcctcaa ggactgcgac gtggtcgtca
     5401 tgctgcgcct gcagaacgaa cgcatgcgcg gcgcgctgct tccttcggca caggaattca
     5461 acaagcacta cggcctgtcc ggcgacaagc ttggggtcgc caggccggac gccatcgtga
     5521 tgcaccccgg tccgatgaat cgcggcgtgg agatcgattc caccgtcgcc gacggtccgc
     5581 actccgtcat cctgccgcag gtcaccttcg gcatcgccgt gcggatggcc gtcatgagca
     5641 tcgtcgccgg caactgatgc gccttcacat caagaacggg cgcatcgtcg acccggcgag
     5701 cggcaaggag ggggccgggg acgttttcat ggtggacggc aaaatttccc cggagggcgg
     5761 gaaggccgac cgggtaatcg atgcgaaggg cctggtcgtc gcgccgggct tcatcgacct
     5821 ctcggcgcgg ctgcgcgagc cgggctacga gtacaaggcg acgctcgagt cggaaatgga
     5881 cgccgcggtc gccggcggcg tgaccagcct tgcctgcccg ccggacaccg atccgccgct
     5941 cgacgagccg ggcctggtcg acatgctgcg ccgccgcgcc aaggcgctgt cgcgcgcgcg
     6001 cgtgtatccc gtcggcgcgc tcacggtcaa gctcgagggc caggcgctca ccgagatggg
     6061 ccagctcacc gatgccggct gcgtcgcgtt ctcccaggcg aacgtgccga tggccgacac
     6121 ccaggtgctg tggcgcgcgc tgcagtacgc cgcgactttc ggctatcccg tgtggctgcg
     6181 cgcggaggac gcgtcgcttg cgaaaggcgg cgtgtcgcac gatggcgagg ttgcgacgcg
     6241 gctcggcctg ccgggcatcc cgtctttcgc cgagaccatc gccctggcga ccctgctcga
     6301 gctcgtgcgc gcgacgcggg cgcgagtgca cgtctgccgg ctgtccacgg ccggcggcgt
     6361 ggagctcatg cgccgggcga agcaggaggg cctgccggtg acttgcgatg tcgccgttca
     6421 tcatgtgcat ctctgcgaca tggacctcgg ctacttcgac tcgcaatgcc gcctcgagcc
     6481 gccgctcagg agccagcgcg accgcgacgc gctgtcgcgc ggcctcgccg agggggtggt
     6541 ggattgcatc tgctccgacc acacgccggt tgacgaggac ggcaagcacc tcccgttcgc
     6601 gcaggccgag cccggcgcga ccgggctcga gcttctcctt ccgttgacgc tgagatgggg
     6661 acaggcgaac aagctcgggc tagccgcgac cctggcgcgc atcacctccg agccggcgcg
     6721 catcctgggc gtcgcgtccg gcaagctcgc gccgggtgcg ccggctgacg tcgtgctgtt
     6781 cgatcccgcc gcgccgttca aggtcgcgcc cgagacgctc aagagccagg gcaagaacac
     6841 gcccttcctc ggctacgagc tcgcaggaag agtgcgcact acgatcgtcg cgggcaacgt
     6901 ggtgtacgag caatgaaacg ggctttttca atcgggcttc ttctatgcgt cgtggggtgc
     6961 ggctcggcct cgaaacccgg tggctactac aaggacgacg gcccgcacga gcggccgccg
     7021 gccaacctgg atcgcgtggc cgacgcagtg ccgcgcaacg agccgttgca caagttcgcc
     7081 aaccggccct atgaggcgct cggccagagg ttcgtgccga tgacttcggt gcagccgttc
     7141 aagcagcgcg gcatggcgag ctggtacgga aagcgctacc acggccagaa gacttcgagc
     7201 ggcgagagct acgacatgta cgcgatgacc gccgcgcatc cgacgctgcc gatcccgagc
     7261 tacgcccgcg tcaccaacgt ggcgaacggc aagagcgtcg tggtgcgcat caacgaccgc
     7321 gggccgttca agtcggaacg ggtcatcgac ctgtcgtacg tagcggccta caagctcggc
     7381 tacatccagg ccggccaggc gcgggtcgaa gtcgaagcga tcgttcccgg caggatcgcc
     7441 ggcttataat tcgcggcttt tcagcacgca ccacatgaga agttcactcg cgctcctcgt
     7501 tgttcttttc tcgttgacgg caacggcagc ggcgccgcag ccgccctccg tcatcggcaa
     7561 gtcatggatg atcggcgatc tctcgagcgg gcaggtgatc gcggcgcaaa aggccgacga
     7621 gcgcttcgag ccggcgtcgc tcacgaagct gatgaccgcc tacgtggtgt tcgcggcgct
     7681 gcgcgaccgc aagctcgcgc ccgatcagca ggtcaatgtc tcgaccaagg catggaaggc
     7741 ggcgggctcg cgcatgttca tcgagccgcg caagccggtg accgtcgacg agctgatccg
     7801 cggcatggtg gtgcagtcgg gcaacgacgc ctgcatcgcg ctcgccgagg cggtggcggg
     7861 aaacgaggaa gtgttcgtgc agatgatgaa ccgcgaggca gctcgcctcg gcatgaagag
     7921 cagcaagttc atgaacgcgt cggggctgcc ggacgcgcag cactacagca ccgcgcagga
     7981 cctgtacacg ctcgccgcgg cgctgatccg cgatttcccg aacgagtacg cgcagtacta
     8041 ctcgcagaaa gagtaccgct acaacaacat cacccagccg aaccgcaatc ggctgctgtg
     8101 gatagacccg agcgtggacg gcatgaagac tggtcacacc gaggccgcgg gctactgcct
     8161 ggtcgcctcg tcgcgccgcg gcggccggcg gctgctctcg gtgcttctcg gctcgacgtc
     8221 cgaggcgaca cgcgcccagg agtcgctcaa gctcctcaac tggggctacc agttcttcga
     8281 cagcgtgaag ctgcatgccg cgggcaaccc ggtgcgctcg ctcgaagtgt ggaaaggcgc
     8341 ggcgcgcaac gtgaaggtgg gcgtaggcaa cgatctcttc gtcactgtgc ccaagggcga
     8401 ggccgacagg ctgaaggcgg agctcgtatc gcagcagccg ctggtcgcgc cgctcggcaa
     8461 gggccagcaa gtcggcgtgg tgcgcgtgac gcacgatggc aagcccttcg gcgagtatcc
     8521 gctgatcacg ctcgagcccg tggctgcggc ggggttcttc ggccggacct gggatacact
     8581 gcgcctatgg ctgaaatgac cgtttttctc aatggaaagc tgctcccgat cgagcaggcg
     8641 acggtgtcgg tgctcgaccg cggcttcatt ttcggcgacg gcgtgtacga gctcgtgccg
     8701 gtgtattcgc gcgtgccgtt ccggctcgag gagcacctgg tgcggctcga gcgcagcctc
     8761 ggcgaggcga agatccgcaa tccgtactcg cgccagcagt ggcgcgcgca cgtctatcag
     8821 ctggccgatg cgcaaccctt cgaggaccag gggatctatt tccaggtcac gcgcggcgtc
     8881 gcgaagcgcg accacgcgtt cccgaagagc gtcgagccca ccgtgttcat gatggcgaac
     8941 ccgctcacca acccgccgca ggcgcagatc gagaacggct cggcggcggt gagcgcgcag
     9001 gacaaccgct ggctgcgctg cgacatcaag tcgatctcgc tcatcggaaa cgtcctcttg
     9061 cgccagctct cggccgaggc gggtgcggcg gagacgatcc tgtttcgcga cggcaagctc
     9121 accgaggctt ccgcgtcgaa cgtgttcatc gtgaagcacg gcgtgatcca gtcgccgccc
     9181 aagaccaacc tcatcctgcc cggcatcacc tacgacgtgg tggtggagct cgcgcgcgcg
     9241 agcagcctcg cctttgagtt cagggacatt ccggaggccg aggtgcgcgc cgccgacgag
     9301 gtctgggtca cgtcctcgtc caaggaagtg ctcgcgatcg tcgagctcga cggcaagcgc
     9361 gtcggcgacg gacgcccggg acccgtgttc cgccgcatgt accagctcta ccaggacttc
     9421 aagcagaaag tcatgcgcgc cggcaagcgg gaagcggtat ccgcttaaga gatcgtcgcc
     9481 cgtgccggtc gtagcggata actcgccgtt agcgtttccc tgcgagtttc cgatcaaggt
     9541 aatgggccgc aagcagccgc gctttgtcca ggcggtgacc agcatcgtgc gcaagcacgc
     9601 accggacttc gacgcagcca ccgtcgagat gcggccgagc cgccagggca ggtacctgag
     9661 cgtcacgtgc accgtgcgcg ccacctcgcg cgagcagctc gacgcgctct acctggagct
     9721 gtgcgaccat cccgacgtgg tcatggtgct gtgacgcgtg gcgtctaccc ttcttgaaga
     9781 gatcgtcgtc aaacggctgg gccgcgccga gtattcgtct gcctgggagg agatgcgcgc
     9841 tttcacgcgg gcgcgcgacg agtcgacgcc cgatgagctc tggatcctcg aacatccgcc
     9901 cgtttacacg gtggggcagg gcgcttcgct ccctcagacc ggaaatgcca tcccggttgt
     9961 gaagaccgac cgcggcggcg acatcactta tcacggacct ggccaggcgg tgatctatac
    10021 gctggtcgat ctcgcgcggc gcgcgatcaa ggtgaagcgc ttcgtgtggc tcctcgagca
    10081 ggcggtgatc gacctgctcg gcggccgcgc cgagcgcaag cccggcgcgc cgggcgtcta
    10141 cgtcgacggc gcaaagatcg ccgcgctcgg catccgtgtg gtccgcggca gggcctacca
    10201 tggccttgcg ctcaacgtcg acatggacct cgcgcccttt gcggccatcg atccgtgcgg
    10261 ctatcccggc ttgcgcgtca cccagatgaa agagctgggc tttgccgact ccccggaaac
    10321 agcggcctcg cggctctcac aaatactggt ggaaaagctc gatgcgtgag gccggcgtaa
    10381 aggaaagagg cgcccgcaag acgatccgca tcgtgcgtca ggaaaacgct acgacgcggc
    10441 tgcccaagcc ggcgtggatc cgggtgcgcg cggcgagccc gaattcgcgc ttctacgaca
    10501 tcaagcgcat tctccgagag cagaagctgc acacggtgtg cgaggaagcc tcgtgcccga
    10561 acatcgccga gtgcttcggc aagggcacgg cgacgttcat gatcctcggc gacatctgca
    10621 cgcgccgttg tcccttctgc gacgtcgcgc acggccggcc gctcgcgccg gatgccgaag
    10681 agcctcgcca tctcgccgag acgatttctc ttcttggctt gtcgtatgtg gtcatcacaa
    10741 gtgtcgatcg ggacgatcta cgagacggcg gcgcggcgca tttcgtggcc tgcattgccg
    10801 aaaccagaaa ggtctcgccg cagaccacga tcgaggtcct ggtgcccgac ttccgcggcc
    10861 ggctcgagcg cgcgctcgag gttctcgatg cggcgccgcc cgacgtgatg aaccacaacc
    10921 tcgagaccgt gccgcggctt taccgcaagg cgcgtcccgg cggcgactat gcgcactcgc
    10981 tgcggctgct tcaagagttc aagcagcgcc accctgccgt gccgaccaag tcgggcctga
    11041 tggtcgggct aggggagacc gacgaagaga tcctcgcggt catgcgcgac ttgcgcgcgc
    11101 acgacgtcga catgctgacg atcggccaat acctgcagcc ttcgccgcat cacttgccgg
    11161 tcgagcggta cgtcgatcct ggaattttca aggaattcga gaatcaggct ctagcgatgg
    11221 gctttcgcca tgccgcggtc ggcgcgctgg tgcgctcgtc ctatcacgcc gaccagcagg
    11281 cgcacgaggc agggatctag cgggcgagct cgcgttccac gatttcgtgg agcgccaccc
    11341 agtcgtgctc gccgacatag cgccgcagca cgcgaccttg ccggtcgatg aggaagctgg
    11401 tcggcgtgat gcgcacgttg ccgaactcct tcgccgcctc gcccgagctg tcgagcgtga
    11461 tcctgaaagg caggccgcgc tttgcggcga acgcggcgac gcgatgcggg ttgtcgtgcc
    11521 gcacggcgac cgcgacggtt tcgtagccgc gcgatgcgaa tttctcgtgg gccgcgacga
    11581 tcttcggcat ctccttcacg caaggcgtgc agtagctgcc ccagaagttc accagcacca
    11641 ccttgccgcg caagtcggat gtggaaaatg tcgcgcccga cagcgggtgg aagcgcactt
    11701 ccggcgccgg cttgagcagc gtgtgcgccg aaagcacgac tattgccgcc gcccagccca
    11761 ctgcggacgc aagggcgccg atgccgacga taagcttcgc tttcatgaga acggcgattt
    11821 tcgcagcgct cgtcatgact gccgccgctc cggccctagg ccagttggag cgtctgaccc
    11881 agcgcgacgc cctcgcgggg ctcaaggccg ctctcgagcg cggctcgcgc gcggcggttg
    11941 ccgagcttgg gcgaaccgac ggcttcttcg gcaaccccca ggtgaagatt ccgatcccgg
    12001 aatcgctcga gcgcaccgaa aagctcgccc ggcgcctcgg tttctcgaac gaggtggagg
    12061 agctggtgga ggcgatgaac cgtgccgccg aggcggccgt gccggaggcg cgcaagctgc
    12121 tgctcgactc ggtgaagaag atgacggtgc aggacgccaa aggaatcctg accggcggcg
    12181 acaccgcggg cacggcgtat ttccgccgca ccgccgacaa gacgctgcac gaccgcttcc
    12241 tcccgatcgt aagcacggcg accgagaagg tcggcgtcgc ccagcgctac aaggagttcg
    12301 cgcgaccggc ggccgcgctc ggc
//
LOCUS       DATBSZ010000032        11313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2257758, whole genome shotgun sequence.
ACCESSION   DATBSZ010000032 DATBSZ010000000
VERSION     DATBSZ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11313
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2257758"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /gene="mog"
                     /locus_tag="VIG70_01985"
     CDS             complement(<1..533)
                     /gene="mog"
                     /locus_tag="VIG70_01985"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEY8249347.1"
                     /translation="MIIGLVSVSDRASAGVYKDEGIPSLKEWLAAAIASPEWRDETRL
                     IADEQPVIEATLKELVDEKGCSLVLTTGGTGPAPRDVTPEATLAVADRVMPGFGEQMR
                     AVSLKYVPTAILSRQVAVVRKKALIINLPGQPKAIRETLDGVFAAVPYCIDLIGGPYI
                     ETHEAVVKAFRPKSALK"
     gene            complement(530..979)
                     /locus_tag="VIG70_01990"
     CDS             complement(530..979)
                     /locus_tag="VIG70_01990"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HEY8249348.1"
                     /translation="MNLQPLFKAEISLAAPQELGATPQGRRRIIGITGGRFAGERLSG
                     RVVPGGADWQVVRADGVADLDARYTLETSDGALIYVRNRGYRHGPAEVLEKLSAGEEV
                     DPTLYYMRTTPLFETGDARYAWLNRIVCIATGARRAAAVELDVFEVK"
     gene            complement(951..1448)
                     /gene="yjgA"
                     /locus_tag="VIG70_01995"
     CDS             complement(951..1448)
                     /gene="yjgA"
                     /locus_tag="VIG70_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEY8249349.1"
                     /translation="MDQEIVSKTRKKREMHELQALGVALAELSESLLKEMRLGEDLLE
                     AVLEAKRIRSHEAKRRQMQYIGRLMREVDPAPIRSRLAELEGSSAQATARHRRLETWR
                     ERLLGDDEALTAFAAEHPGADLQALRALIRNARKEQKESKPPRAYRELFRVLKEIESP
                     TPLQG"
     gene            1469..2824
                     /gene="pmbA"
                     /locus_tag="VIG70_02000"
     CDS             1469..2824
                     /gene="pmbA"
                     /locus_tag="VIG70_02000"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEY8249350.1"
                     /translation="MPKRHGFSCERAELDSLAQAVLERARRAGANGCDCDVSEGYGLT
                     VTVRKGKPDTIEHNRDRSIGVTVYFGERPKARRGHASTSDFSRTALEQTVDAAAAIAR
                     HTAEDDCAGLPDAELLAREAPDLDLYHPWPLSTEDAVDLAKRCEAAAFAVSKKIRNSE
                     GATVSAQQTQFAFANSLGFSGGFAGSRHWLSCAVIAEEKGLMQRDDWYSASRVPAKIA
                     DPRALGRYAGSRAAARLGGRKIATCQAPVLFEAPVAIGLIGHFVSAVNGGNLYRKTSF
                     LVDSLGKDVFSPLVTIDERPLEPQGMASSAFDEEGVATCERSIVRNGVVDGYFLGSYA
                     ARKLGMKSTGSAGGHHNLIVKSDGPDFEGMLRKMRRGLLVTELLGQGVNLVTGDYSRG
                     AAGYWVEGGEIAFPVEEITIAGSLKEMFKQIAAIGSDVLVRSGRSSGSILVENMTIAG
                     E"
     gene            2898..3173
                     /locus_tag="VIG70_02005"
     CDS             2898..3173
                     /locus_tag="VIG70_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249351.1"
                     /translation="MGHACTHHDGPDPHEHPVIAVIAIIAIIAAMLLIAWARLEVHGT
                     EDIEAALTEPAVQGDPSAAPRRDLKGHEEREVDAAVTSSRFATGFSP"
     gene            3189..3773
                     /locus_tag="VIG70_02010"
     CDS             3189..3773
                     /locus_tag="VIG70_02010"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8249352.1"
                     /translation="MRTLIAVTAAVLAAAPVLALEPNAPYQYLAQAQPRKPAQTPDVI
                     YVPTPHEVVDDMLRLANVGKGDILYDLGSGDGRIAIAAAKKFGIKAVGIDIDPERIRE
                     ATENARKAGVGNLVEFRQEDLFKADFREATVVTLYLLPDLNVKLRPRLWEELKPGTRI
                     VSHQFDMGTWKPEKRLDSNGRTVYFWTVPAAKKK"
     gene            3775..4377
                     /locus_tag="VIG70_02015"
     CDS             3775..4377
                     /locus_tag="VIG70_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEY8249353.1"
                     /translation="MKSLQIEVASDVVCPWCYIGKRRLEKALALVQDLKFEVRWLPFQ
                     LNPDMPKEGMPRAEYRKAKFGSLERSRALDARVIAEGRSEGIEFAFERIERTPNTAAA
                     HRLIFLAQDQDPVVDALFRAYFEEAKDIGDAAVLAGIAARCGVEGWPGKARDVSGLEE
                     EVRGLGISAVPTFVIERKLGVSGAYPAESLAAAMRQAAAR"
     gene            complement(4370..4966)
                     /locus_tag="VIG70_02020"
     CDS             complement(4370..4966)
                     /locus_tag="VIG70_02020"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8249354.1"
                     /translation="MPIKPERRAAALLYAELRNFTRLSEVLQPDKVLDLANEFFSMAS
                     RAAVAGAGNVIAVHNDSLLASFAQGDARQYNEAALNTAQAIRGQFGSVGERWQKEYGL
                     AAAVALGVHSGEAVFGMAGPLGAQQFVAFGDCVSVAERLVHRARAGEIVISIDVMKAL
                     GNAVQALGAQELPALELGGKRRPLPIYGLLLETRLDFT"
     gene            complement(4979..5668)
                     /locus_tag="VIG70_02025"
     CDS             complement(4979..5668)
                     /locus_tag="VIG70_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004526445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEY8249355.1"
                     /translation="MAELVFRDDPYRVACEATVVSVQDNVVELDRTVFYPLGGGQAGD
                     TGRLGKWRVLDTRKGAAGDSVLHLLEPGAKLAAGDPVEIQIDWERRHRLMRLHTALHL
                     LGAVVKAPVTGGRIGDDKAHLDFDIEMEKLVKEAIEARLHELISSSLETRPTWITDAE
                     LDERPELVKTMSVAPPRGEGRVRLLEIPGVDLQACGGTHVKNTAEIGRLAVARIRSEG
                     KRNKRVTLTFA"
     gene            complement(5661..6257)
                     /locus_tag="VIG70_02030"
     CDS             complement(5661..6257)
                     /locus_tag="VIG70_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEY8249356.1"
                     /translation="MEMTGEQLIPASQQDTWTALNDPEVLKACVPGCESITRVNDNEY
                     QVQMTTRVGPVSAKFRGRLSLFDIKPPQSYSLAFEGQGGAAGFAKGAAQVKLSPEGAK
                     TRLAYDVKANVGGKLAQIGSRLIDAAAKKVADEFFQNFTKRMTSSEDTTVVMHAVDAP
                     EGAAAGDRAMPSVSNTTLIFFAAGALVVVIVALVALFG"
     gene            complement(6248..7435)
                     /locus_tag="VIG70_02035"
     CDS             complement(6248..7435)
                     /locus_tag="VIG70_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8249357.1"
                     /translation="MTASGRLPENVMHFARLLRAAGMRVGPDRVIDCVRALEIAGAHQ
                     FPLRRDDWYCTMSAVLLSRQEQRPIFDQAFRIFWRDPKLAERMMSLMLPQAYGRTPKP
                     EQQQSQRLTDALFAQKTESPETERLELEARLTFSSREILSRMDFDTMSAAELAEAKKM
                     LAELRLPLPLIRTRRFRAHPSGRRIDLRKTLRASLREGGDLIPLVRAAPAELHPPLVV
                     LCDISGSMNPYSRMFLHFLHAITNDRDRVAVFVFGTRLTNITRQLRHRDVDVAMAKVA
                     DAIKDWSGGTRIGASLREFNFKWGRRVLGQNACLLLVSDGLDREAGEGLGEEMERLAK
                     SCHRLVWLNPLLRYEKFEARPAGVRAMLPHVDLFLPVHNLKSLVDLARTLSTPFKENP
                     EWK"
     gene            complement(7443..8759)
                     /locus_tag="VIG70_02040"
     CDS             complement(7443..8759)
                     /locus_tag="VIG70_02040"
                     /inference="COORDINATES: protein motif:HMM:NF017135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HEY8249358.1"
                     /translation="MSASSFLRRLTGGRGSMGGAAQNLFALCDALLAESGEYASTALA
                     REALEAYQRLDERGRREFFDVLARDYSPSPEAVAQSAAAYQADPSPDNLAQLQESVEP
                     LRRELFRRLNAAQGGTAALVELRRELLSNLKANPHWRVIDADLLQLLREWFNRGFLRL
                     ERIDWRTSAIVLEKLIQYEAVHAIQGWRDLRRRLETDRRCFAFFHPQLPDEPIIFIEV
                     ALTRTMSAHIQPLLDIGSPVTPANCDCAMFYSITNCQEGLRGISFGNLLIKRVAEELR
                     REFPHLRRFATLSPIPGFRRWLEQKRSERPIELAALARLEQPDWHVGEISDSLQRALL
                     RLCAYYLLNARQGEVRESEPLDPVARFHLGNGAVLERLNWMGDISENGIQRSAGLMVN
                     YIYWLNELERNHERYWRESVIAASPQVEKLARECVLAPAAEKGAAA"
     gene            complement(8764..9672)
                     /locus_tag="VIG70_02045"
     CDS             complement(8764..9672)
                     /locus_tag="VIG70_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8249359.1"
                     /translation="MQKPLPKSIDETQALLAAGEYVADRSLATSLFLSLAMKRPLFLE
                     GEAGVGKTEIGKVVAQALGRDLIRLQCYEGLDIAQAAYEWNYPRQMIEIRMAEAEGVK
                     SREGLEQDIFNERFLVRRPLLRALQSAGAPPVLLIDELDRTDEPFEAYLLEVLSDWQI
                     TIPEIGTVRAAEPPVVVITSNRTREIHDAVKRRCFYHWVDYPDARRELEILRRKAPQA
                     AEALSREVVSFVQQLRKVDLFKLPGVAETIDWANCLAALDRVALDPETVNNTLGVLLK
                     YRDDLERIAGEEAGRLVGEAKAAAAA"
     gene            9776..10747
                     /locus_tag="VIG70_02050"
     CDS             9776..10747
                     /locus_tag="VIG70_02050"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8249360.1"
                     /translation="MTSRRDVLALLGLAPWLAHAQQRRVYRVGVLETTALRLNTENFD
                     RFRKGMEERGYVEGPSLVLLYRSAEGQTERFDELAAELVNQKVDLILTRGTPATLAAK
                     RATATIPIVMAAVADPVESKLAASLARPGGNVTGLTTASAELVPKRLELFKALVPKMT
                     RVAAYANLSNVAAAATWKDLETAARAMGLEPMLLDVRAPEQIAPAFADAVRQGAQGLV
                     VGIETLAQSNRNAIVELAAQHRLPAIYSGREFVEAGGLMSYGVSYPDLYYRAAGFADR
                     VLKGAVPGELPIERPAKFAFAINRRTAKTLNIVIPPDILLRADWAIE"
     gene            10820..>11313
                     /locus_tag="VIG70_02055"
     CDS             10820..>11313
                     /locus_tag="VIG70_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249361.1"
                     /translation="MEMQMVKELLLQSLEHEMGGTQVYETALKCVVNEDLKEEWEKYR
                     EETEKHVQVLHDACLQLELDPEEQTPGRKITHDIGASLIAAMEAALGTGDKEMAQCVA
                     AECVVHAETTDHFNWQLIGEVAKKMTGAQGKALKEAYEEVEDEEDEHLYHSKGWLREL
                     SLDGL"
BASE COUNT         1809 a         3847 c         3873 g         1784 t
ORIGIN      
        1 ttcttcaggg cggacttggg gcggaaggcc ttgaccacgg cctcgtgggt ctcgatgtag
       61 ggaccgccga tcaggtcgat gcagtagggc acggcggcga acaccccgtc gagggtctcg
      121 cggatcgcct tgggctgtcc cggcaggttg atgatcagag ctttcttccg caccaccgcc
      181 acctggcgcg agaggatcgc ggtgggcacg tacttgaggc tcaccgcgcg catctgctcg
      241 ccgaacccgg gcatgactct gtcggcaacc gcgagggtcg cttccggtgt gacgtcgcgc
      301 ggcgcgggcc cggtgccgcc ggtggtcagg accaggctgc agcctttctc gtcgaccagc
      361 tctttcagcg tggcctcgat caccggctgc tcgtcggcga tcaaccgggt ctcgtcgcgc
      421 cactcgggcg aagcgatcgc ggccgcgagc cattccttga gcgacggaat gccttcgtcc
      481 ttgtacacgc ccgccgaggc gcggtcgctg acggaaacca ggccgatgat catttgactt
      541 caaaaacgtc gagctcgacc gcggcggcgc gccgtgcacc ggtcgcaatg caaacgatgc
      601 ggttgagcca ggcgtagcgg gcgtcgccgg tctcgaagag cggcgtggtg cgcatgtagt
      661 acagggtggg atccacttct tctcccgcgg agagtttttc caggacctcg gcggggccgt
      721 ggcggtagcc ccggtttctg acgtagatca aagccccgtc ggaagtctcg agggtatagc
      781 gcgcatccag gtcggcgacg ccgtcggcgc gcacgacctg ccagtccgcg ccgccgggaa
      841 ccacgcgccc cgacaggcgc tcgccggcga agcgcccgcc ggtgatgccg atgatccgtc
      901 gccgtccctg cggagtcgcg ccgagctcct ggggcgcggc gagcgagatc tcagccttga
      961 agaggggttg gagattcaat ctctttcagg accctgaaga gctcgcggta ggcgcgcggc
     1021 ggcttgcttt ccttttgctc tttcctggca ttgcgaatca aggcgcgcag cgcttgcagg
     1081 tcggcgcccg gatgctcggc ggcgaacgcg gtaagcgcct cgtcgtcgcc gagcaggcgc
     1141 tcgcgccagg tctcgaggcg ccggtggcgc gccgtggcct gcgccgagct gccctcgagc
     1201 tccgcgaggc ggctgcggat cggcgccgga tcgacctcgc gcatgaggcg cccgatgtac
     1261 tgcatctgcc ggcgcttcgc ctcgtggctg cggatgcgct ttgcttcgag caccgcttcg
     1321 agcaggtctt cgccaagccg catttccttc agcagcgatt cggaaagctc ggccagcgcc
     1381 acgccgagcg cctgcagctc gtgcatttcg cgtttcttgc gcgtcttgct gacaatttcc
     1441 tgatccacgc gattatcata gcgcccccat gccgaagcgc cacggtttca gctgcgagcg
     1501 ggccgagctc gacagcctcg cgcaggcggt gctggagcgc gcccggcgcg ccggcgcgaa
     1561 cggctgcgac tgcgacgtgt cggagggcta cggcctgacg gtcaccgtgc gcaagggcaa
     1621 gcccgatacc atcgagcaca atcgcgatcg ctcgatcggc gtcacggtct acttcggcga
     1681 gcggccgaag gcgcgccgcg gccatgccag cacctcggac ttctcgcgca ccgcgctcga
     1741 gcagacggtc gacgccgcgg cggcgatcgc gcgccacacc gccgaggacg actgcgccgg
     1801 cctgcccgac gcggagctcc tcgcgcgcga ggcaccggac ctcgatctgt atcacccgtg
     1861 gccgttgtcc acggaggacg cggtggatct cgcgaagcgc tgcgaggccg cggccttcgc
     1921 ggtgtcgaaa aagatccgca actccgaggg cgcgacggta tcggcgcagc agacccagtt
     1981 cgcgttcgcg aacagcctcg gcttctcggg cggctttgcg ggatcgcgcc actggctgtc
     2041 gtgcgcggtg atcgccgagg aaaaagggct gatgcagcgc gacgactggt acagcgcctc
     2101 gcgcgtgccg gcgaaaatcg ccgaccctcg ggcgctcggc cgctatgccg gcagccgcgc
     2161 ggcggcgcgc ctcggggggc gcaagatcgc cacctgccag gcgccggtgc tgttcgaagc
     2221 gccggtcgcg atcggcctga tcgggcactt cgtctcggcg gtgaacggcg gcaacctgta
     2281 ccgcaagacg tcgttcctgg tggacagcct gggcaaggac gttttctctc ccctggtgac
     2341 gatcgacgag cgcccgctcg agccgcaggg catggcgagc agcgccttcg acgaggaagg
     2401 cgtggcgaca tgcgagcgct cgatcgtgcg caacggcgtg gtagacgggt atttcctcgg
     2461 cagctacgcg gcgcgcaagc tcggcatgaa atcgaccggc agtgccggcg ggcaccacaa
     2521 ccttatcgtt aagtccgacg gtccggattt cgagggaatg ctgcgcaaga tgcggcgcgg
     2581 tctcctggtc accgagctgc tcgggcaggg agtgaacctg gtgaccggcg actactcgcg
     2641 cggggcggcg ggctactggg tcgagggcgg cgagatcgcc ttcccggtcg aggagatcac
     2701 catcgccggc agccttaaag aaatgttcaa gcaaatcgcg gccataggga gcgacgtgct
     2761 ggtgcgcagc ggccgctcgt cgggctccat cctggtcgag aacatgacca tcgccgggga
     2821 gtaattacaa acagatatta gtgaggaggt tttacttcct tccccgaacc cggcgaccgg
     2881 agtagcgtct aatccgaatg ggccacgcct gcacgcacca cgacggcccg gatccccacg
     2941 agcatccggt gatcgccgtc atcgcgatca tcgccatcat cgccgccatg ctgctgatcg
     3001 cctgggcgcg tctcgaggtg cacggcaccg aagacatcga ggctgcgctc accgagccgg
     3061 ccgtccaggg cgacccgtcg gcggcaccga ggcgggatct gaaaggccac gaagaacgcg
     3121 aggtggacgc ggcggtcacg tcgagccgtt tcgccaccgg cttttcgccc tagggcataa
     3181 tttccgggat gcgaaccctc attgcagtaa ccgcggcggt actcgccgcg gcgccggtcc
     3241 tcgccctaga acccaacgcc ccctaccagt acctcgccca ggcgcagccg cgcaagcctg
     3301 cgcagacgcc cgacgtcatc tacgtgccca cgccgcacga ggtggtcgac gacatgctgc
     3361 gcctcgccaa cgtgggcaag ggcgacatcc tctacgacct gggctcgggc gacggccgca
     3421 tcgccatcgc cgcggcgaag aagttcggca tcaaggcggt cggcatcgac atcgacccgg
     3481 agcgcatccg cgaggcgacc gagaatgcgc gcaaggccgg ggtcggcaac ctcgtggagt
     3541 tcaggcagga ggatctgttc aaggccgact tccgcgaggc cacggtggtc acgctgtacc
     3601 tgctgcccga cctcaacgtg aagctgcggc cgcggctctg ggaagagctc aagcccggga
     3661 cgcgcatcgt ctcgcaccag ttcgacatgg gcacctggaa gcccgagaag cgcctcgact
     3721 cgaacggccg cacggtgtac ttctggaccg tccccgcggc gaagaaaaag tagggtgaag
     3781 tccctgcaga tcgaggtcgc ctcggatgtc gtctgtccct ggtgctacat cggcaagcgc
     3841 cgtctcgaaa aagcgcttgc gctggttcag gacctgaagt tcgaagtgcg ctggcttccc
     3901 ttccagctca atcccgacat gccgaaagag ggaatgccgc gggccgagta ccgcaaggcc
     3961 aagttcggct cgctcgagcg ctctcgcgcg ctcgatgcgc gcgtcatcgc cgagggcagg
     4021 agcgagggca tagagttcgc tttcgagcgt atcgagcgca cgcccaatac cgctgccgcc
     4081 caccggctga ttttcctggc gcaggaccag gacccggtgg tcgatgcgct gttccgcgcc
     4141 tatttcgagg aagcgaagga catcggcgac gctgcggtgc tcgccggcat cgccgcgcgc
     4201 tgcggcgtgg aaggctggcc cggcaaggcg cgcgacgtgt cggggctcga ggaagaggtg
     4261 cgcgggctgg gcatttcggc ggtgccgacc ttcgtcatcg agcgcaagct cggcgtgtcc
     4321 ggggcctatc cggcggagtc gctcgccgcc gcgatgaggc aagccgccgc taggtgaagt
     4381 cgaggcgggt ctcgagcagc aggccgtaga tcggcagcgg gcggcgcttg ccgccaagct
     4441 cgagcgccgg cagctcctgg gcgccgaggg cctgcaccgc gttgccgagc gccttcatca
     4501 cgtcgatcga gatcacgatc tcgccggcgc gcgcgcggtg caccaggcgc tcggcgacgc
     4561 tgacgcagtc gccgaaggcg acgaactgct gcgcgccgag cggcccggcc atgccgaaga
     4621 ccgcctcgcc gctatgcacc ccgagcgcga cggccgccgc caggccgtac tccttctgcc
     4681 agcgctcgcc caccgacccg aactgccccc ggatcgcctg cgcggtgttg agcgcggcct
     4741 cgttgtactg ccgggcatcg ccctgcgcga aggaagcgag cagcgaatcg ttgtgcaccg
     4801 cgatcacgtt gccggcgccg gcgaccgccg cgcgcgacgc catcgagaag aactcgttgg
     4861 cgagatcgag gaccttgtcg ggctgcagca cctcggacag ccgcgtgaag ttgcgcagct
     4921 cggcgtagag cagcgcggcg gctctgcgtt caggcttgat cggcatgcgc taccgcgctc
     4981 atgcgaaagt cagggttacc cgcttgttgc gctttccttc ggagcggatg cgcgccaccg
     5041 cgaggcggcc gatctcggcg gtgttcttca cgtgcgtgcc gccgcacgcc tgcaggtcga
     5101 cgcccggaat ctcgagcagc cgcacgcgcc cctcgccgcg cggcggcgcc accgacatgg
     5161 tcttcaccag ctcgggccgc tcgtcgagct cggcgtccgt gatccaggtc ggtcttgttt
     5221 cgagagaaga tgaaatcaac tcgtgcaggc gcgcttcgat ggcctccttc accagcttct
     5281 ccatctcgat gtcgaagtcg aggtgcgcct tgtcgtcgcc gatgcgccct ccggtgaccg
     5341 gcgccttcac caccgcgccg agcaggtgca gcgcggtatg cagccgcatc agccggtggc
     5401 gccgttccca gtcgatctgg atttccaccg ggtcgcccgc agcaagcttt gctcccggct
     5461 cgagcaggtg caggaccgaa tcgccggccg cgcccttgcg cgtgtcgagg acgcgccact
     5521 ttcccagccg gccggtgtcg cccgcctggc cgccgcccag ggggtagaac acggttcggt
     5581 cgagctcgac cacgttgtcc tggaccgaga ccacggtcgc ctcgcaggcg acgcgatacg
     5641 ggtcgtcgcg aaagactagc tcagccaaag agcgccacca aggcgacgat caccaccacc
     5701 agcgcgcccg cggcgaagaa gatgagggtg gtgttcgaga ccgagggcat ggcgcgatcg
     5761 ccggcggcgg cgccttcggg cgcgtccacg gcgtgcatca ccacggtcgt atcctcggac
     5821 gaggtcattc tcttggtgaa gttctggaag aactcgtcgg cgaccttctt cgccgccgcg
     5881 tcgatcaggc gcgagccgat ctgcgcgagc ttgccgccga cgttcgcttt cacgtcgtag
     5941 gcaagccggg tcttcgctcc ctcgggagaa agcttcacct gcgcagcgcc cttcgcgaag
     6001 cccgccgcgc cgccctggcc ttcgaaggcg agcgagtagg actgcggcgg cttgatgtcg
     6061 aagagcgaaa gccggccgcg gaacttggcg ctcaccgggc ccacccgggt ggtcatctgc
     6121 acctggtact cgttgtcgtt cacccgggtg atcgactcgc agcccggcac gcaggccttg
     6181 aggacctccg ggtcgttgag cgccgtccac gtatcctgct gcgacgccgg gataagctgc
     6241 tcgcccgtca tttccattct ggattttcct taaagggcgt gctcagggtt cgcgcgaggt
     6301 cgaccaggct cttcagatta tgcaccggca ggaacagatc gacgtgcggc agcatcgcgc
     6361 gcacgcccgc cggccgcgcc tcgaacttct cgtagcgcaa gagcgggttg agccacacca
     6421 ggcggtggca cgacttggcg aggcgctcca tctcctcgcc gaggccctcg cccgcctcgc
     6481 ggtcgaggcc gtcggacacc aggagcaggc acgcgttctg ccccagcacg cgccgtcccc
     6541 acttgaagtt gaactcgcgc aggctcgcgc cgatgcgcgt gccgccggac cagtccttga
     6601 tcgcgtcggc caccttcgcc atcgccacgt cgacgtcgcg gtgccgcaat tgccgcgtga
     6661 tgttggtgag gcgggtgccg aacacgaaca ccgcgacgcg gtcgcgatcg ttggtgatcg
     6721 cgtgcaggaa gtgcaggaac atgcgcgagt aagggttcat cgagcccgag atgtcgcaca
     6781 gcacgacgag cggcgggtgc agctcggcgg gcgcggcgcg caccagcgga atgaggtcgc
     6841 cgccctcgcg caggctcgcg cgcagggtct tcctcaggtc gatgcgtctt ccggacggat
     6901 gcgcgcggaa gcggcgggtc cggatcagcg gcagcggcag gcgcagctcg gccagcatct
     6961 tcttcgcctc ggccagctcg gcggccgaca tggtgtcgaa gtccatgcgc gagaggatct
     7021 cgcgcgagga gaaggtgagc cgcgcctcga gctccagccg ctcggtttcc ggggactcgg
     7081 tcttctgggc gaacagcgcg tcggtcagcc gctggctctg ctgctgctcg ggcttcggcg
     7141 tgcggccgta ggcctgcggc agcatcaggc tcatcatgcg ctcggcgagc ttggggtcgc
     7201 gccagaaaat gcggaacgcc tggtcgaaga tcgggcgctg ctcctggcgc gacagcagca
     7261 ccgccgacat cgtgcaatac cagtcgtcgc gccgcagcgg gaactggtgc gcgccggcga
     7321 tctcgagcgc gcgcacgcag tcgatgaccc ggtcggggcc gactcgcatg cccgccgctc
     7381 gcagcagccg ggcgaaatgc atcacgtttt ccgggagtct tcccgaagcc gtcatcgcca
     7441 cttcaggcgg cggcgccttt ctccgccgcc ggcgcgagca cgcattcgcg cgccagcttc
     7501 tcgacctggg gcgaggcggc gatgacactc tcacgccagt agcgctcgtg gttgcgctcg
     7561 agctcgttca gccagtagat gtagttgacc atcagcccgg ccgagcgctg gatgccgttc
     7621 tcggagatgt cgcccatcca gttcaggcgc tcgagcaccg ccccgttgcc gaggtggaag
     7681 cgcgccaccg ggtcgagcgg ctccgactcc ctcacctcgc cctgtcttgc gttcaagagg
     7741 tagtaggcgc acagccgcag cagtgcgcgc tgcagcgagt ccgagatctc gcccacgtgc
     7801 cagtcgggct gctccagcct tgcgagcgca gcgagctcga tcggccgctc gctccttttc
     7861 tgctcgagcc agcggcggaa tccggggatc ggcgagagcg tcgcgaagcg acgcaggtgc
     7921 gggaactcgc gccgcagctc ctcggcgacg cgcttgatca gcaggttgcc gaacgagatg
     7981 ccgcgcagcc cttcctggca gttggtgatc gaatagaaca tcgcgcaatc gcagttcgcc
     8041 ggggtcaccg gcgagccgat gtcgagcagc ggctggatgt gcgcgctcat ggtgcgcgtc
     8101 agcgccacct cgatgaagat gatcggctcg tcgggcagct gcggatggaa gaacgcgaag
     8161 cagcgccggt cggtctcgag gcgccggcgc aggtcgcgcc agccctggat ggcgtgcacc
     8221 gcctcgtact ggatcagctt ctccagcacg atcgcggaag tgcgccagtc gatgcgctcc
     8281 aggcgcagga acccgcggtt gaaccactcg cgcaggagct gcagcaggtc cgcgtcgatg
     8341 actcgccagt gagggttggc cttcaggttg ctcagcagtt cccggcgcag ctcgaccagc
     8401 gcggccgtgc cgccctgcgc cgcgttgagg cgccggaaaa gctcccggcg caacggctcg
     8461 acggattcct gcagttgcgc caggttgtcg ggggagggat cggcctggta ggcggctgcg
     8521 ctctgggcga ccgcctcggg ggagggcgag taatcgcgcg ccagcacatc gaagaactcg
     8581 cgccggccgc gctcgtcgag ccgctggtaa gcctcgagcg cctcgcgcgc gagcgcggtg
     8641 ctcgcgtact cgccgctttc ggcgagcagc gcgtcgcaca gcgcgaacag attctgcgcg
     8701 gcgccgccca tgctgccgcg cccgccggtg agccgccgca gaaaggacga ggcgctcatg
     8761 ccgctacgcc gccgccgcgg ccttcgcctc gccgaccagc cggccggctt cctcgccggc
     8821 gatgcgctcg agatcgtcgc ggtacttgag cagcacgccg agcgtgttgt tgacggtctc
     8881 gggatcgagc gccacgcggt cgagcgccgc caggcagttg gcccagtcga tggtctccgc
     8941 gacgccgggc agcttgaaca ggtccacctt gcgcagttgc tgcacgaacg acaccacctc
     9001 gcgcgacagt gcttccgccg cctgcggcgc cttgcgccgc aggatctcga gctcgcgcct
     9061 ggcgtccggg tagtcgaccc agtggtagaa gcagcgccgc ttgaccgcgt cgtggatctc
     9121 gcgcgtgcgg ttggaggtga tcaccaccac cggcggctcg gcggcgcgca cggtgccgat
     9181 ctccgggatc gtgatctgcc agtcggacag cacttccagc aggtaggcct cgaacggctc
     9241 gtcggtgcgg tcgagctcgt cgatcagcag caccgggggc gcgccggccg actgcagcgc
     9301 gcgcagcagc ggccgccgga cgagaaagcg ctcgttgaag atgtcctgct ccaggccctc
     9361 gcgcgacttc acgccctcgg cctcggccat ccggatctcg atcatctggc gcgggtagtt
     9421 ccactcgtag gccgcctgcg cgatgtccag gccctcgtag cactgcagcc ggatgagatc
     9481 ccggccgagc gcctgcgcca ccaccttgcc gatctcggtc ttgcccaccc cggcctcgcc
     9541 ctcgaggaag agcggccgct tcatcgccag cgacaggaac agcgacgtcg ccaggctgcg
     9601 gtcggccacg tactcgcccg cggcgagcag cgcctgggtc tcgtcgatcg acttcggcag
     9661 gggtttctgc ataaagcgtt gcattttccg gcatttggcg aaacgccgcc accccgggtt
     9721 cgctcgctga actcgtgcaa cacatccgta ccgaggaggc gcagactagc ggcgaatgac
     9781 gagccgccgc gacgtgcttg cgctccttgg cctcgcgcct tggctggcgc atgcccagca
     9841 acgcagggtt tatcgcgtcg gggtgctcga gacgaccgcg ctgcggctca ataccgagaa
     9901 cttcgaccgc ttccgcaagg ggatggagga gcgcgggtac gtcgaagggc cgagcctcgt
     9961 gctcctgtac cgctcggccg aagggcaaac cgagcgcttc gacgagctcg ccgcggagct
    10021 ggtcaaccag aaggtggacc tgatcctgac gcgcggaacg cccgcgacgc ttgcggcaaa
    10081 gcgcgctacg gcgacgattc cgatcgtcat ggcggcggtg gccgatccgg tggaaagcaa
    10141 gctggccgca agcctcgcgc ggccgggcgg caacgtcacc gggctcacca ccgcgtcggc
    10201 ggagctggtg cccaagcgcc tcgagctctt caaggccctg gtgccgaaga tgacgcgcgt
    10261 tgccgcgtac gccaacctca gcaacgtcgc cgcggccgcg acctggaagg atctcgagac
    10321 cgcggcgcgc gcgatgggtc tcgagccgat gcttctcgac gtgcgcgccc ccgagcagat
    10381 cgccccggcg ttcgcggacg cggtgcggca gggcgcgcaa gggctggtcg tcggcatcga
    10441 gacgctcgcg cagtcgaacc gcaacgcgat cgtcgagctc gccgcccagc accgcctgcc
    10501 cgccatctat tcggggcggg agttcgtcga ggcgggcggc ctgatgtcgt acggggtgag
    10561 ctatcccgat ctgtactacc gcgccgccgg cttcgccgat cgcgtcctca agggcgccgt
    10621 cccgggcgag ctgccgatcg agcgccccgc gaagttcgcg ttcgcgatca accggcgaac
    10681 cgcgaaaacc ctcaatatcg tcattccgcc cgacatcctc ctgcgcgccg actgggcgat
    10741 cgaataggcg tcgggaattc cccgatcggc atggcgcttg ctcccacgtg ggcgccggca
    10801 accgaaagga gagatttcca tggaaatgca aatggtcaag gagctgctat tgcagtccct
    10861 cgagcatgag atgggcggta cccaagtcta cgagacggcg ctcaagtgcg tcgtcaatga
    10921 ggacctgaag gaagaatggg agaaataccg ggaagaaacc gagaagcacg tgcaggtctt
    10981 acacgacgcg tgcctgcagc tcgagctcga tcccgaggag cagacgccgg ggcgcaagat
    11041 cacgcacgac atcggcgcct cgctgatcgc ggcgatggaa gccgcgctcg gcaccggtga
    11101 caaggaaatg gcgcagtgcg tggccgcgga atgcgtggtg cacgccgaga ccaccgacca
    11161 cttcaactgg cagctgatcg gcgaggtggc gaagaagatg accggcgcgc agggcaaggc
    11221 gctgaaggaa gcctacgaag aggtcgagga cgaggaagac gagcacctct accactccaa
    11281 gggctggctg cgcgagctct cgctcgacgg cct
//
LOCUS       DATBSZ010000033         5368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2258013, whole genome shotgun sequence.
ACCESSION   DATBSZ010000033 DATBSZ010000000
VERSION     DATBSZ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258013"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VIG70_02060"
     CDS             complement(<1..795)
                     /locus_tag="VIG70_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY8249362.1"
                     /translation="MADALAAHAAKRDSLIEALRQARGPNEGPVALAKDTSNLFRDRA
                     ASARRRLDVRGFNQVLSVRDGFVEAEGMTAYEDLTRECLAHGVMPSVVPQLKTITLGG
                     AVAGVGIESSSHRQGLVHDGMLELDVLLGDGRVVSCTPHNAHADLFFGFPNSYGTLGY
                     ALRVKAKTIPVRRYVRLSHLPFTDPDAYFSELEKHVRAGVADFIDGTIFSPQRLFITL
                     GRFTESAPYTSDYTYENIYYRSIAQKREDYLTVHDYLWRWDTDWFWC"
     gene            complement(788..2047)
                     /locus_tag="VIG70_02065"
     CDS             complement(788..2047)
                     /locus_tag="VIG70_02065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY8249363.1"
                     /translation="MRSWIEQTLRQVGERTAVPFAVRFADGVEIRTQDAPPAFTLVFR
                     NSRTYWRMAAFGHVGLLEAYFDGDVDIEGSLAKALAAGMEGQIDRRGTPFTWIRNRWH
                     ELTHSNAERSQARRNANFHYALTPEFYKLWLDDPLLLYTCAYWTEGTRTLEEAQRNKC
                     DHVARKLLLKPGEEVVDVGSGFGGFMFHAQERYGVKVTAINTTGSQAEHARREIERRR
                     LGNALSMQMHEDFRDAGRQFDKVASIGCLEHAGRGQLGELIRAHAGYLKKGGLGLIHF
                     IGHVGRYDTDFFIRKHVFPGGWIPSLADVIVEMERAGLEILDIENLRRHYALTLDVWA
                     ERFDRNWERIRALDPQRFDERFRRIWRVYLYGCAEMFRSPAGRTHLFQIVFSKGNVTQ
                     SSYPMGRDFLYEEIAELASAPRRVGHG"
     gene            2135..2398
                     /locus_tag="VIG70_02070"
     CDS             2135..2398
                     /locus_tag="VIG70_02070"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEY8249364.1"
                     /translation="MAAKKDTERRATERRAPPRTTPNTAKPSAFNDSARADISADELR
                     KLISEAAYYRAKRRGFAPGHELEDWVQAEAEVIGRLSSPGTSH"
     gene            2478..2741
                     /locus_tag="VIG70_02075"
     CDS             2478..2741
                     /locus_tag="VIG70_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249365.1"
                     /translation="MKICVLSRTGRGGQYDPQVFHLGQRRLPVVAVLEQWTDAQHRFF
                     KVRVADGRRFVLRHDPATGAWELAAAYGGESGSSPALAALVRI"
     gene            complement(2738..3523)
                     /locus_tag="VIG70_02080"
     CDS             complement(2738..3523)
                     /locus_tag="VIG70_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8249366.1"
                     /translation="MTKRLNPSPAQRLSAVAIAAALTAFAGAANAQPSSSPGLPGYAV
                     SSDGRPVLSGFGQCVRSGHWVPANAAEPCDRIARADVPPPAPVAAAPEPKLEPAPAAA
                     AAPVVVVPPRPVIQKLTLSTDVLFDFGKADLKDSGKSRLDQLAGEIKDAQVDEIIAVG
                     HADRIASEDYNQKLSEARAQAVKEYLGTKVANANRVTAEGKGESQPVTGDDCKKMGAE
                     RGSNKKLVACLQPDRRVEIEVLGSRQVAGGTPDAGAGATSTSR"
     gene            3688..3840
                     /locus_tag="VIG70_02085"
     CDS             3688..3840
                     /locus_tag="VIG70_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249367.1"
                     /translation="MSHLYTVLGILCAALLFGLAACEREGPAEKAGAAVDRAAKDVGD
                     ALKGKK"
     gene            3945..4367
                     /locus_tag="VIG70_02090"
     CDS             3945..4367
                     /locus_tag="VIG70_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEY8249368.1"
                     /translation="MKALKGSKTHDNLKAAFAGESQANRRYLYFASKADVEGHNDVSA
                     LFRSTAEGETGHAHGHLEYLEQVGDPATNLPIGRTRENLKAAVAGETHEYTDMYPGMA
                     KQARDEGFGEIADWFETLAKAERSHANRFQKALDGLSD"
     gene            4367..>5368
                     /locus_tag="VIG70_02095"
     CDS             4367..>5368
                     /locus_tag="VIG70_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY8249369.1"
                     /translation="MREGSLEAPTRHPLDWKNPAFWDPAAAEKELERIFDICHGCRRC
                     VSLCTAFPTLFDLVDSSPTLEVDGVAKADYAKVVDQCYLCDLCYMTKCPYVPPHPWNV
                     DFPHVMLRAKAIKFRQGESSRNPLRDRLLSSTDALGKLAAIPVVAQAINALNGNSLSR
                     SAMQAVLGVHKDRELPPYAPKKFRNHAAQSPSFPAKDGSNTPGKVAIFATCYANYNEP
                     AMGHDLAAILAHNEIPYVIVESEVCCGMPKLELGDLESVEKHKAANIPVLAKYAREGW
                     AIVTAVPSCTLMFKQEIPLLFPGEEDVKLVRDAMFDPFEYFVLRKKDGLLKTGFKQAL
                     "
BASE COUNT          911 a         1785 c         1813 g          859 t
ORIGIN      
        1 gcaccagaac cagtcggtgt cccagcgcca cagatagtcg tgcaccgtga ggtagtcctc
       61 gcgcttctgt gcgatcgagc ggtagtagat gttctcgtag gtgtaatcgc tcgtgtaggg
      121 agcggattcg gtgaagcggc cgagcgtgat gaacaggcgc tgcggcgaga agatcgtgcc
      181 gtcgatgaaa tcggcaacgc ccgcccgcac gtgcttttca agctctgaaa aataagcgtc
      241 cggatccgtg aagggcagat gcgaaaggcg cacataccgc ctgaccggaa tcgtcttcgc
      301 cttgacccgc agcgcatagc cgagcgtgcc gtacgagttc gggaagccga agaacaaatc
      361 cgcgtgcgcg ttgtgcggcg tgcagctcac gacgcggccg tcgccgagca gcacgtcgag
      421 ctcgagcatg ccgtcgtgga ccaggccctg gcggtgcgac gaggactcga tgccgacgcc
      481 ggcgaccgcg ccgccgagcg tgatcgtctt cagctgcggc acgaccgagg gcatcacgcc
      541 gtgcgccagg cattcccgcg tcaggtcctc gtacgcggtc atgccttcgg cttcgacgaa
      601 gccgtcccgg accgacagca cctggttgaa gccccgcacg tcgagccgcc ggcgcgcgct
      661 cgccgcgcga tcgcgaaaca ggttggaggt gtccttggcg agcgcgaccg ggccctcatt
      721 tggaccccgg gcttgccgta gagcctcgat cagggaatcg cgcttcgcag cgtgcgccgc
      781 cagcgcgtca gccatgcccg acgcggcggg gagcgctggc gagctcggcg atttcctcgt
      841 aaaggaagtc gcggcccatc gggtagctcg actgcgtcac gttgcccttc gagaacacga
      901 tctggaagag gtgcgtgcgc ccggccggcg agcggaacat ttcggcgcag ccgtagaggt
      961 acacgcgcca gatgcggcgg aagcgctcgt cgaagcgctg cgggtcgagc gcccggatgc
     1021 gctcccagtt gcggtcgaaa cgctcggccc agacgtcgag ggtgagagcg tagtggcgcc
     1081 gcaggttctc gatgtcgagg atctccaggc cggcgcgctc catctccacg atcacgtcgg
     1141 cgaggctcgg gatccagccg ccggggaaca cgtgcttgcg gatgaagaag tcggtgtcgt
     1201 agcgcccgac gtggccgatg aagtggatca ggccgaggcc gcccttcttc aggtagcccg
     1261 cgtgcgcgcg gatcagctcg ccgagctggc cgcggccggc gtgctccagg cagccgatcg
     1321 acgcgacctt gtcgaactgg cggccggcgt cgcggaaatc ctcgtgcatc tgcatcgaga
     1381 gcgcgttccc caggcgccgg cgctcgattt cgcgccgcgc atgctcggcc tgcgagccgg
     1441 tggtgttgat cgcggtgact ttgacgccgt agcgctcctg cgcgtggaac atgaagccgc
     1501 cgaagccgct gccgacgtcg acgacttcct cgccgggctt gagcagcagc ttccgcgcca
     1561 cgtggtcgca cttgttgcgc tgcgcctcct cgagcgtgcg cgtcccctct gtccagtagg
     1621 cgcaggtgta gagcaacagg ggatcgtcca gccagagctt gtagaactcg ggcgtgagcg
     1681 cgtaatggaa attggcgttg cgccgcgcct gcgagcgttc ggcgttcgaa tgcgtgagct
     1741 cgtgccagcg gttgcggatc cacgtaaaag gcgtgccgcg gcggtcgatc tggccctcca
     1801 tgccggcggc gagggccttg gcgaggctgc cttcgatgtc gacgtcgccg tcgaagtagg
     1861 cctcgagcag gccgacgtgg ccgaaggcgg ccatgcgcca gtaggtccgc gaattgcgaa
     1921 acaccagcgt aaacgccggc ggggcgtctt gcgtgcggat ctccacgccg tcggcaaagc
     1981 gcaccgcgaa aggaacggcg gtgcgctcgc cgacctgccg cagggtttgc tcgatccagg
     2041 atctcacagg ggccaaattt taccgtccgg tagaagcggg ggcacaactt ctgctagcct
     2101 cggtttcacc cccccgcaag acaggagtcc ccccatggca gccaaaaagg ataccgagcg
     2161 ccgtgccacc gagcgtcgcg caccgccgag gaccacaccc aataccgcca agccctcggc
     2221 tttcaacgac agcgcccggg ccgacatctc ggctgacgag cttcgtaagc tgatctcgga
     2281 ggccgcctac taccgcgcca agaggcgcgg gttcgcgccc ggccacgagc tcgaggactg
     2341 ggtccaggcg gaagccgaag tcatcgggcg tctgagcagc cccgggacct cccactaggc
     2401 tccgaacggc cgcagtccga ccgggctatg gcgccgggtc ggctaattgc tgcgttcctg
     2461 cgttgcagga gcgtgcaatg aagatatgtg ttttgagccg cacgggtcgc ggcgggcagt
     2521 acgacccgca ggtgttccac ctcggccagc gccggcttcc ggtcgtcgcc gttctcgagc
     2581 agtggacgga cgcgcagcac cgttttttca aggtccgggt cgcggacggg cggcgcttcg
     2641 tgctgcgcca cgacccggcc acgggcgcgt gggagctcgc ggccgcctac ggcggcgagt
     2701 cgggctcgtc gcccgccctc gccgccctgg tccggatcta gcgcgacgtc gacgtcgccc
     2761 cggccccggc gtcaggtgtt ccgccggcga cctggcggct ccccagcacc tcgatttcca
     2821 cgcgccggtc aggctgcagg caggccacga gcttcttgtt gctgccgcgc tcggcgccca
     2881 tcttcttgca atcgtcgccg gtcaccggct gggactcgcc tttgccttcg gcagtgacgc
     2941 ggttcgcgtt ggcgaccttc gtgcccagat actccttcac cgcctgcgcg cgggcttccg
     3001 aaagcttctg gttgtaatcc tcggacgcga tgcggtcggc gtggccgacg gcgatgatct
     3061 cgtccacctg ggcgtccttg atctcgccgg cgagctggtc gagccgcgac ttgccggagt
     3121 ccttcaggtc ggccttgccg aagtcgaaca gcacgtcggt cgacagcgtc agcttctgga
     3181 tcaccgggcg gggcggcacg acgacgaccg gggccgcggc cgcggctggc gcaggctcga
     3241 gcttgggctc gggcgcggcg gcgacggggg ccgggggcgg cacgtccgcg cgcgcgatgc
     3301 ggtcgcacgg ctcggcggcg ttcgccggaa cccagtggcc gctgcgcacg cactggccga
     3361 acccgctcag caccgggcgt ccgtccgaag agacggcgta gccggggagc ccagggctgg
     3421 aagacggttg cgcgttcgcg gcgcccgcga acgccgtcag cgctgcggcg atcgccacgg
     3481 cgctcaggcg ctgcgctggg gagggattga gtcgcttggt catagttgct gtcctttccg
     3541 aggaggcact gcaaaaagtt ccgcggcccg gaccatatga cccgccgccg catccaagta
     3601 gcaggggaaa gggagcaact aacgtgcctc gaccggacta ttgcgcaccc cgacgtattg
     3661 ctgcagtgcg cgcgtagcct tgccggcatg agccacctct acaccgtcct cggaatcctg
     3721 tgcgcagcgc tgcttttcgg cctcgcggcc tgcgagagag aaggcccggc cgagaaggcc
     3781 ggagcggccg tcgaccgcgc cgccaaggac gtcggcgacg ccctcaaggg caagaagtga
     3841 cggcaagaat ccggcattga gagaaacgat cgatcgaatt acgaaattcg atttgatcta
     3901 ttgagcgcgg ggccgtaggc tcgtcctccc tacaggagga ccccatgaaa gcgctcaaag
     3961 gcagcaagac ccacgacaat ctgaaggccg ccttcgccgg cgaatcgcag gcgaaccgcc
     4021 gctatctcta tttcgcttcc aaggccgacg tcgagggcca taacgacgtc tcggcgctgt
     4081 tccgctcgac cgcggaaggc gagaccggcc atgcgcacgg ccacctcgag tacctcgagc
     4141 aggtcggcga cccggccacc aacctgccga tcgggaggac tcgggaaaac ctaaaagccg
     4201 cagttgccgg cgagacccac gagtacaccg acatgtaccc ggggatggcg aagcaggcgc
     4261 gcgacgaagg cttcggcgag atcgccgact ggttcgagac gctggccaag gccgagcgca
     4321 gccacgccaa tcgcttccaa aaagcattgg atggtctctc cgactgatgc gtgagggttc
     4381 gctcgaggcc ccgactcggc acccgctcga ctggaagaac ccggcgttct gggacccggc
     4441 ggcggccgag aaggagctcg agcgcatctt cgacatctgc cacggctgcc gccgctgcgt
     4501 cagcctgtgc acggcgtttc cgacgctgtt cgacctggtg gattcgtccc ccaccctgga
     4561 ggtcgacggc gtcgcgaaag ccgactacgc caaggtcgtc gaccagtgct acctgtgcga
     4621 cctgtgctac atgacgaagt gcccctacgt tccgccgcac ccgtggaacg tcgactttcc
     4681 gcacgtcatg ctgcgcgcga aggcgatcaa gttccggcag ggcgagagct cccgcaaccc
     4741 gctgcgcgac cgcctgctct cctccaccga tgcgctgggc aagctcgccg cgattccggt
     4801 ggtggcgcag gctatcaacg cgcttaacgg gaactccctt tccaggagcg cgatgcaggc
     4861 ggttcttggc gtgcacaagg accgcgagct gccgccctac gcgccgaaga aattcaggaa
     4921 tcacgcggcg cagtcgccat cattccccgc gaaggatggc agcaacacgc ccggcaaggt
     4981 ggcgatcttc gccacctgct acgcgaatta caacgagccg gcgatggggc acgacctggc
     5041 tgcgatcctc gcccataacg aaattccgta cgtgatcgtg gagagcgagg tctgctgcgg
     5101 catgccgaag ctcgagctcg gcgatctcga atccgtggaa aagcacaagg cggcgaacat
     5161 ccccgtgctg gcgaagtacg cccgcgaggg ctgggccatc gtcacggcgg tgccgagctg
     5221 cacgctcatg ttcaagcagg agatcccgct cctgttcccc ggcgaggagg acgtgaaact
     5281 cgtgcgcgac gcgatgttcg atccgttcga atatttcgtg ctgcggaaga aggacggcct
     5341 gctcaagacc gggttcaagc aggccctc
//
LOCUS       DATBSZ010000034        53701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_277056, whole genome shotgun sequence.
ACCESSION   DATBSZ010000034 DATBSZ010000000
VERSION     DATBSZ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 53701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53701
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277056"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..560)
                     /locus_tag="VIG70_02100"
     CDS             complement(6..560)
                     /locus_tag="VIG70_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249370.1"
                     /translation="MSARFVFLSLVLAGCASVQPLINPVDAVVGEAITTARAPAAEQA
                     AALARAEKQARSAGTNPLDWLRLATFLATLPPPLRDDARALELLGPLADAASPGVGRF
                     AALLSSQIVERQRIARELDHLAREARERDRLDKERDRVDKERDRLDKERDKREEALKQ
                     QLEALRAIERGILEREEKLRRRQK"
     gene            complement(557..1981)
                     /locus_tag="VIG70_02105"
     CDS             complement(557..1981)
                     /locus_tag="VIG70_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8249371.1"
                     /translation="MAYPRSFLGLCLASFVLVAAPLIAALAYSAWHSERLAEQSRHAV
                     FNASQAARASRTLVNRISSLERLAMLNAPGLDSDLGRVHTSFVLVAQELLQLPLEEDQ
                     LGALKKTMAQEQGLYDLLAAPRSGPRAVAERRQVAALAADLAENASEMLAISTRVADR
                     EVLRLRAEAESVQRQLVLLVIFSTAVALALALALTRFIARPIAELDAAIRQLGGADFA
                     QPISVRGPEDLKTLGERLDWLRRRLVELEDEKNRFLRHLSHELKTPLTALREGAELLH
                     DEVGGPLSGSQRRVVSIMKDNSIKLQRLIEELLDYQRALHAAAALAPRRLALDALVRE
                     VARGHELAAQAKGQRLALELERVQVNADPEKLRSIVDNLIGNALKFTPAGGSVAVLAR
                     SSGDEAVIDVIDSGPGVPAEERDSIFDSFFRGRAKASARVEGSGLGLAIAREFVEAHG
                     GKISVLAEERGAHFRVALPRRMAA"
     gene            2024..2524
                     /locus_tag="VIG70_02110"
     CDS             2024..2524
                     /locus_tag="VIG70_02110"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY8249372.1"
                     /translation="MSQASGPRIYLVAAVAANGIIGAKGKLPWHIPEELKHFKRLTLG
                     HPVIMGRRTWESLKGPLPQRENIVVTRTPGYEAPGAAVASSLGAALALCIGEPVAFVI
                     GGTQLFEEALPHAAGMVLTEIQRDYEGDTWFPAWDRAQWRESQREAHAAVDGTRFDFV
                     LYEKKP"
     gene            complement(2521..3735)
                     /locus_tag="VIG70_02115"
     CDS             complement(2521..3735)
                     /locus_tag="VIG70_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEY8249373.1"
                     /translation="MGDRSSSITHRQSAAVLKQQQSQEARFDTHAEVSLDFELVREAA
                     HQKYTRPFLILDTAIVRSKIRRFRAAMPRVRPHYAVKANPDRRVLKVLAQEGAGFEIA
                     STAELDLLLGLGVPAAEVFYSNPMKSREAIAYAAAKGVEWYVVDSVDELRRVHEVKPD
                     AKQYLRIATPNIGSDWPLSGKFGCGVADAREIVAAAAKMGVDLAGVTFHVGSQCRNPE
                     NWRVALEKAKALFDAMGKAGLKPRLLNIGGGFPVRHVKPIPSIEVIGEVVNEGLKAFP
                     EDVQVIAEPGRYLVSDAGYFVCRVLGTATRAGKRWMHWDAGLFGGVIESSEGLRYRVR
                     TDRSGPDVAWTVGGPTCDSVDVVMREEPLPSDLQEGDFIYIQNAGAYTTAYASQFNGF
                     PLPEVRVFESRS"
     gene            complement(3938..4501)
                     /gene="dcd"
                     /locus_tag="VIG70_02120"
     CDS             complement(3938..4501)
                     /gene="dcd"
                     /locus_tag="VIG70_02120"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013522100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY8249374.1"
                     /translation="MTIKSDKWIRRMAAKGMIEPFEAGQVKAFEGKKIVSYGTSSYGY
                     DVRCSTEFKIFTNINSTIVDPKHFDEKNFVDYRGEVCIIPPNSFALARTVEYFRIPRS
                     VLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPARVYANEGVAQVIFLE
                     SDEVCETSYRDRGGKYQGQKGVTLPRI"
     gene            4524..5627
                     /locus_tag="VIG70_02125"
     CDS             4524..5627
                     /locus_tag="VIG70_02125"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY8249375.1"
                     /translation="MRTIAVLAALLSSGAFAADALFTPGEIRAILAHGPWPAPATSDP
                     SNRVSGRRDAIELGQRLFFDPRLSVNGKFSCGTCHVPERNWTDNRVRGVAAAEVDRNT
                     PTLMNVRLGQWYGWDGGADSLWSQSIRPILDRRELGATPRHVADLMRQDEDLSCRYRR
                     AFGAAPSPTDDEEVLVNVGKILAAFQETFETPPTPFDRFRNALARGEPLKAGLYSEAA
                     QRGLKIFVGKGNCSSCHSGPNFTSGEFRNNGFSADAGRVEGLKAVKASRFNLAGPYND
                     DPLRRVHAAQEPEAGAFKVPTLRHLMLTAPYGHDGKRETLAEVVRHYSEKGSTALPAL
                     KLTAAEQTDLVVFLESVSTLSNPWRPEDHARCQ"
     gene            5630..6475
                     /locus_tag="VIG70_02130"
     CDS             5630..6475
                     /locus_tag="VIG70_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY8249376.1"
                     /translation="MSLSVRPLAPNLGAVISGTDLSCELDDRVFRELYQAFLRYQVLL
                     FPPQDLPPASQVAFARRFGEVQVHVMNQYHADGYPELYRLSNLDENGKPHGRHPDKGT
                     LAWHTDGSWQRVTGQATIIYGEVMPQSGGETHFCDMYGAYERLDASWKARIANLRAVH
                     NLDFSRTRRHGEDPLTEAQRLAKPPVDHPVVRTHPETGRKCLYLGDHAEYIQGMPYDE
                     GRALIEELNALAVHPDLTYEHRWKPRELLVWDNRCVMHRATAYDAATQGRVIRRCTVL
                     GEVPA"
     gene            complement(6541..7626)
                     /gene="apbC"
                     /locus_tag="VIG70_02135"
     CDS             complement(6541..7626)
                     /gene="apbC"
                     /locus_tag="VIG70_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEY8249377.1"
                     /translation="MSVNEGSVQEALRQLVDPNTGKDYVASRSARNIKVSGSDVALEI
                     ELGYPGKSQHEPIRRQVTQALKSVGAANVSVKVSSKVVSHAVQRGVKLVPGIKNIIAV
                     ASGKGGVGKSTTAVNLALALAAEGASVGVLDADIYGPSQPMMLGIAGRPESKDGKRIE
                     PMEGHGLQASSIGFLIDIDTPMVWRGPMVTQALEQLLKDTNWRDLDYLVVDLPPGTGD
                     IQLTLAQKVPVTGAVIVTTPQDIALIDARKGLKMFEKVGIPILGVVENMSFHTCPKCG
                     HESHIFGSGGAERMAKDYKTELLGQLPLDESIRSQTDSGKPTVVSDPDGELAAIYRRI
                     ARRCAVKIAESQRDMTSKFPNIVVQNT"
     gene            7662..9671
                     /gene="metG"
                     /locus_tag="VIG70_02140"
     CDS             7662..9671
                     /gene="metG"
                     /locus_tag="VIG70_02140"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY8249378.1"
                     /translation="MISHAKARKLFVTTALPYANGPFHIGHIMEYIQADIWVRFQRMQ
                     GHEVHFVGADDSHGAPIMLAAEKAGKSPDAFIEAIQKGREPYLEGFHISYDNWHSTHS
                     PENTELSQDIYRKLKAAGLVYAKPVEQFFDPVKRMFLADRYIKGECPNCGAKDQYGDA
                     CENCSKAYSATELKNPYSALSGAKPVLKSSEHHFFKLSDPKCKSFVQEWLEKPGRLQS
                     QVVNKAKEWLEGTGDKALADWDISRDPPYFGIRVPDIQEEKYLYVWLDAPIGYLASFK
                     NYCGKAGLDFERFLQDPKTEQIHFIGKDIIYFHTLFWPAMLKFAGKPYKVPDHVYVHG
                     FITVSGDKMSKSRGTGIDPLRFLKLGMNPEWLRYYIAAKLNANVEDLDFNPDDFLTRV
                     NADLVGKYVNIAARAASFISRQFDGALHPSNDVSLALTSARAIAEAYEQREFGRVIRE
                     AMRIADLVNQRYDQLKPWELAKDPAKREELHRVCSDAIRAFFVLTVYLAPILPATASR
                     VARELFGFKRDFTWADASALPDRIAPFKHLITRVEEAQLDALFEPPQEKTVEQTTLSI
                     DDFNKLDLRVARIARAEQVEGADKLVKLTLEVGDGTRTVFAGIKSDYAPEKLEGRLAV
                     LVANLAPRKMKFGVSEGMVLAASGDGPGIFLVSPDAGARPGMRVK"
     gene            9710..10822
                     /locus_tag="VIG70_02145"
     CDS             9710..10822
                     /locus_tag="VIG70_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEY8249379.1"
                     /translation="MDSAGRTLVCSVLFLDIADYSQKPVAEQLQLKQAFNQALGVALE
                     HVLSRDRIILDTGDGAAITFMGDPEDALFASMAMRDLATSVPVRSGVNLGPVRLVKDL
                     NGQMNIIGDGINVAQRVMSFSRPGQLLVSRSFYEVVSCLSRDYASLFRHEGSRTDKHV
                     RAHEVYAVVGATPAPRRVDEAEARSESTPPGAVGWLSGMGPFGLRRSALAAAPLAFLL
                     IVAGGGVARALMPDEPVVKPQPPRTGAKPAAPAAPAAGSAAPAAGGAAPIAGGGAAVI
                     EEPPKQKKQIVARAEKKPEKPAAAKGPGRLELAVAPWGEVLVDGKSRGLSPPLRVLEV
                     TPGPHTIEIRNSTFPAHVARVEVKPGESVKIQHRFR"
     gene            10822..11736
                     /locus_tag="VIG70_02150"
     CDS             10822..11736
                     /locus_tag="VIG70_02150"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HEY8249380.1"
                     /translation="MGRALFLLLFIVLAGCAQLDQLVVATKARLTLVEGLRQFDAGDH
                     VTSAKTLASALEMGLSDKDRANAHKHLAFIHCAAGRERQCREEFRKALAADPATRLDA
                     AEAGHPVWGPIFRTLSGSAALEAGLKQYESGEYAEAAKNLQGAVDLGVSDKQRANAHK
                     HLAFIHCTANRERQCREEFRKALAADPALQLDAAEAGHPVWGPIFRSMNGPSALSAGL
                     KLYESGNYEESAKNLQVAIELGVPEKLRANAHKHLAFIHCSSNRERQCRDEFRKALAA
                     DPSTDLDAAEAGHPVWGPIFRSLKGAAK"
     gene            11767..12966
                     /locus_tag="VIG70_02155"
     CDS             11767..12966
                     /locus_tag="VIG70_02155"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8249381.1"
                     /translation="MEQPQLGRYVIDSEIGRGAMGVVFKATDTVLQRTVAVKTVNMAL
                     EKDHADKYEARFYQEARAAGGLNHPNIVTVYDAGKAGDVVYMAMEYIQGVELRTLFVE
                     GQPMGVPQALSIAAQVAEGLGYAHQQGVVHRDVKPANIMVVSEGPVKITDFGIARMRA
                     SADLTQTGVMLGSPKYMSPEQVIGKRADHRSDIFSLGVILYEMLTGAAPFSGENVTAL
                     MYQIVNFAPPAPSTVNRQVPEMLDFVVAKMLAKPLDERYQDAREAARDLRECERSLAG
                     PASTTLSGRPLGLASGTQPEMMSTQSKAVVLAQSVNRTRQLDEKTPAEAGAPLARGVS
                     NSFDSNEATQRLAALTGSATIPQTATQAIGAVPHPPRGRWRRRDWLVVGGATLAGLIV
                     AGLIARR"
     gene            12963..13637
                     /gene="can"
                     /locus_tag="VIG70_02160"
     CDS             12963..13637
                     /gene="can"
                     /locus_tag="VIG70_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEY8249382.1"
                     /translation="MRDLQALFRNNRRWAERILRADRRFFDKLARQQSPRYLWIGCSD
                     SRVPANEIVGLLPGELFVHRNIANMVVHSDLNCLSVIQFGVEVLKVRHIIVCGHYGCS
                     GVRAAVMKERHGLVDNWLRHIQDVRHEHAPRLRAVRARARLVDRLCELNVIEQVANVC
                     RTSIVEDCWARRQPLTVHGWIYGIHDGLLRDLGFTAGSPAQAASGYRRAITVSGRRSP
                     RAAAGG"
     gene            complement(13591..14004)
                     /locus_tag="VIG70_02165"
     CDS             complement(13591..14004)
                     /locus_tag="VIG70_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEY8249383.1"
                     /translation="MRKTLALLVLAAGCASVEPQQDRVVYHLSEGLPQASNGLRNIGN
                     HLEVNPKARIVVVSHARGVDFLMKDAKDANGNRYEDLVEQLKMRGVRFDVCEITLRNR
                     KLAKDQFIAEVSWVPSGVAEVTRLQQREGFAYLKP"
     gene            complement(14009..14404)
                     /locus_tag="VIG70_02170"
     CDS             complement(14009..14404)
                     /locus_tag="VIG70_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249384.1"
                     /translation="MRAGLLVIAGLALIGFVAVAVVLPRMQGSEMREAAQALVAGAEA
                     AKQQVGSAAEKSGSLATAGNGIKALENDHPKHGKMKWIVSQNGAIRGWNEKNALEVTL
                     TPSLQGGKASWSCKGYPVDAMPPTCGGKG"
     gene            14465..14743
                     /locus_tag="VIG70_02175"
     CDS             14465..14743
                     /locus_tag="VIG70_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY8249385.1"
                     /translation="MATQRLRITGRVQGVGFRYALQDEAQKLGLAGWVRNRRDGSVEA
                     LAQGSPPALAALATWARRGPPAARVTDVRVEAAPDEPATEGFELRPTL"
     gene            complement(14740..15483)
                     /locus_tag="VIG70_02180"
     CDS             complement(14740..15483)
                     /locus_tag="VIG70_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249386.1"
                     /translation="MPAASRFVEALKRSVRARGLTYAELARRLRISEASVKRMFSRGT
                     FTLSRVEEVLAAVDLDLYEVARMSRGGGAGPAQLTHEQELGLAGDERLLAVFWLLLNG
                     WSFEEILEGYAVARTELTIAFARLEKLKLIEWGPRERARLLVPRDFLWRAGGPAKKAY
                     ARRAMAEFLQARFDGPLELIRFEPRELSAESAATLKRKLEQLLAEFGEMAEADSALPA
                     SRRTPVAMLAACRAWQFSAVSAIRRRKNA"
     gene            15621..16532
                     /locus_tag="VIG70_02185"
     CDS             15621..16532
                     /locus_tag="VIG70_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249387.1"
                     /translation="MSALELSAAVREGRSYDAARLDRLLRVDEGHGLVEVQASTPWKA
                     IAASLRPGDPRAAGTHTTMSTVGDSLAHNAAGPDGRPAVLHVESIAVVTPGGELRRAS
                     RTAHRELFALVAGGQGLFGALYSVTLDIGSLSRAVNNAVERSGRPVVPGRGLRLLLPP
                     EKLQAFLDEARGRCEEWRMALAGVEARHTVPENDSFLRWARREYAAVTLHLAEPAALG
                     AAVRATQLRRSLIDAAIVQGGSFPVSCTPEATRAQAEACYPQLREFLAEKRRIDPGER
                     LVNAWYRHYRTLFGSPCAVRWAHGAVN"
     gene            complement(16529..19909)
                     /gene="dnaE"
                     /locus_tag="VIG70_02190"
     CDS             complement(16529..19909)
                     /gene="dnaE"
                     /locus_tag="VIG70_02190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY8249388.1"
                     /translation="MAAPRFVHLRLHSEYSVSDGIVRIEEAVERARADAMPALALTDA
                     GNLFGMVKFYGAARAAGVKPVIGVDCWLQNDADRDKPFRLLLLCASRAGYLRLCDLLS
                     RAWLRNQHRGRAELAREWLQDAEGLIALSGAASGDVGQALLAGHDEAAERLAQGWAAL
                     FPGRYYLELQRAGQPHAEALLARSVALASRLGLPVVATHPVQFLDAGDFRAHEARVCI
                     ATGHILGDQRRPRRFTPEQYFKRQDEMARLFADIPQALENSIEIARRCNLAIELGKSR
                     LPAFPTPAGVGVEDYLRQQAHDGLKKRGLQNVERLEFELKTIIQMGFAGYFLIVADFI
                     NWAKQNGVPVGPGRGSGAGSLVAYSLGITDLDPLKYDLLFERFLNPERVSMPDFDIDF
                     CQDGRDRVIDYVRQKYGEGSVSQIATFGTMAARAVLRDCGRVLDLPYSFCDQLAKLVP
                     VQPGKLITLDDARKAEPLLAEREKNEDEVRELLELSEKLEGLVRNVGMHAGGVLIAPG
                     RLTDFCPLYAADGAQVISQLDKDDVEAIGLVKFDFLGLTTLTILDWAERFVRGLGQAD
                     FSLAQVPLDDAQTYKLLSSGNTTAVFQLESRGMRDLIKRARPDRFEDVIALVALYRPG
                     PMDLIPEYIERKHGKRVDYLDPRLEPILGPTYGIMVYQEQVMQIAQVMGGYTLGGADL
                     LRRAMGKKKPEEMALQRDSFVEGAQKKGLARGKATQLFDLMEKFAGYGFNKSHAAAYA
                     LLAYQTAYMKTHHAAAFAAANLSAVMDDTDKVRQFHEDALANGLTILPPDVNSSEYRF
                     VPVDRGTIRYGLGAVRGTGQSAIESILSARKEAPFTSLFDFCSRVDKRMVNRRVLEAL
                     VRAGAFDAIDANRARLLASAGRAFEAAEQAERQASQESLFGEAEAPRGGAHVTVEAPP
                     WDTRQKLLEEKAALGFYLSAHLFSIYERELSRFPRTALAKLSPGERVWMAGIVVAARA
                     QMTRRGRMMVVMLDDATAQVEISVFNELFERHREKLKEDALLVVCGRVQDDQFSGGLR
                     VLAEELLDLDGLRARYAARLKISMNGGADAKRLQQVLAPYRATGNGACLVVVAYGNGN
                     AACEVVLGEGWRVRPDSKLLADLGDWLTPENIELVFGS"
     gene            19939..20253
                     /locus_tag="VIG70_02195"
     CDS             19939..20253
                     /locus_tag="VIG70_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249389.1"
                     /translation="MKRSGIVLSILAAIALSASALAQDRTAELDKAVEEARTAYLELQ
                     EAEKRRDQGIESQAGERQGSAGGGSRPNDAYFGRQAQLEQEVELARKRYEAAQKRWND
                     LK"
     gene            20275..21153
                     /locus_tag="VIG70_02200"
     CDS             20275..21153
                     /locus_tag="VIG70_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8249390.1"
                     /translation="MNPAPNSALLALLLGATCIALSPIFVRVSEAGPTATAFWRVALA
                     VPVLWILYALKSKARTRRYSGKWPLLLAAGFAFAGDLAFWHTSIKLTSVANSTLLANL
                     ASIFVTLAAWTFLRQRPSGLFLAGLAAALAGVALLVHTSLEFSATGLAGDALGVVTAM
                     FYAGYILAVKGLRDRGETTLHLMAVTTTITALFLFPVALASGEPMLPASAFGWWMLVG
                     LALISHAAGQGLIAYALAHLPAAFSSVSLLFQPVMAALFAWLLLAEGLVPLQVLGGII
                     VLGGIYLARRGSSAGQ"
     gene            21174..21587
                     /locus_tag="VIG70_02205"
     CDS             21174..21587
                     /locus_tag="VIG70_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249391.1"
                     /translation="MAKAKKKAKSKLRRKKPLKKVVKKVLKAVKKVLKKPRPKAAKPA
                     ISAPAPLPGERIGIVTHYFGHLSVAVVKLEPGVTLRVGDNIHVKGHTSDFSQRVESLQ
                     IGHASVEQVGTDDDFGLKVAEHAREHDVVYRVKPS"
     gene            complement(21584..22609)
                     /gene="purM"
                     /locus_tag="VIG70_02210"
     CDS             complement(21584..22609)
                     /gene="purM"
                     /locus_tag="VIG70_02210"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY8249392.1"
                     /translation="MSCIVKFQSSMSKGLSYRDAGVDIDAGDALVEAIKPFARRTMRP
                     EVLGAIGGFGALCEIPKKYRHPVLVSGTDGVGTKLKLAFQLDRHDTVGIDLVAMSVND
                     VLVQGAEPLFFLDYFASGKLDVQVAASVIQGIAKGCEQAGCALIGGETAEMPGFYAAG
                     EYDLAGFCVGVVEKERIIDGRSIRAGDALLGLASSGPHSNGYSLIRKILERAKPPFDL
                     LTPTRIYVKPVLKLLEGVAVKGLAHITGGGLTGNVPRMLPKDLLATIHKAAWPRPEVF
                     QWLQREGKVAEDEMAGVFNCGIGMVLAVERADVQRAMESLRASGETVYEIGAIEKSSG
                     EPDAVIV"
     gene            22599..23204
                     /locus_tag="VIG70_02215"
     CDS             22599..23204
                     /locus_tag="VIG70_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEY8249393.1"
                     /translation="MQLILDLAAAVEPDFDNYVAGPNAEAVASLRSFAAGATRDAVMY
                     LWGEPGSGRTHLLRSAAKANPGLVTADDVDDLDEPAQQGLFAAINAAREGAPPVLAAG
                     SRPPAQLALREDLRTRLAWGLVYQLKPLSDAEKALHLRAEAARRGLRLPDEVVGYLLT
                     RLPRDLPSLNSVLDALDRYSLAAKRPVTLPLVREALERKFR"
     gene            23284..23697
                     /locus_tag="VIG70_02220"
     CDS             23284..23697
                     /locus_tag="VIG70_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249394.1"
                     /translation="MKARAALFLLFLFAGQTLGQSGLRLPQQGEPGLDPTFARGWLAP
                     DYDRFGFALYTWREAVGFAPGQRMQLTYPFGDRGSLGMSMASGTYYVDENRQVSLYGS
                     YSLSADWALSAETLGRDASGLFRLQDFRIGVQRRF"
     gene            23728..24390
                     /locus_tag="VIG70_02225"
     CDS             23728..24390
                     /locus_tag="VIG70_02225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8249395.1"
                     /translation="MRLALFDLDNTLLAGDSDYEWGQFMCDQGVVDRAAYEAQNRKYY
                     EQYKAGTLDIHEFLGFALRPLANHTATELERWHAQFMRARILPMITPAARTLVRRHLQ
                     AGELCAVITATNSFVTAPIAREFGIEHLIATEPERRNGRFTGRVAGTPCFRDGKLVRL
                     DEWLAGLGRRLADFEASTCYSDSHNDLALLERMTRPVAVDPDETLAREAARRGWERIS
                     LR"
     gene            24406..25731
                     /gene="pcnB"
                     /locus_tag="VIG70_02230"
     CDS             24406..25731
                     /gene="pcnB"
                     /locus_tag="VIG70_02230"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEY8249396.1"
                     /translation="MIKRFIRRVLGLASEGMQRVPRERHGLARERLSPAAAKVCAVLR
                     EAGFSAYVVGGAVRDLLLGIVPVDFDIATDARPEQVKPLFRRALIIGRRFRLVHVMMG
                     PETVEVSTFRSADDTSEKDEHGRVLRDNVFGTQEGDALRRDFSVNALYYDPATEEVVD
                     FHGGLADLKKRVMRVIGDAETRYREDPVRMLRGVRLAAKLGLTLDPHTREPIRSLAPL
                     MERVPPARLFDEMLKLLLSGHASACLRQLREVGLHKGLLPLLDVILEQPLGERFVTLA
                     LAQTDERVLADRPVSPAFLFAALLWHEVLAAWKSRQARGERAIPALETAMDEVLDTQC
                     AKLAITRRLTATMREVWAMQPRFDGRSGNRAFRTLEAPRFRMAYDFLALRAASGEVPA
                     ELEAWWRTFQSADADTRKAMLLPDTAPRKRRRRRRKKGPAAAASAPETA"
     gene            25728..26195
                     /gene="folK"
                     /locus_tag="VIG70_02235"
     CDS             25728..26195
                     /gene="folK"
                     /locus_tag="VIG70_02235"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY8249397.1"
                     /translation="MTIAYVGIGSNLEEPETQVRKAFDELDRMPHTRLVKKSSLYRSA
                     PVGHAAQPDFVNAVARLETGLPAERLLAELQEIEDRHGRRRSFANAPRTLDLDVLLYG
                     ERSLDLPGLKIPHPRMHERAFVLRPLLEISPDLQPRFNACLDACADQKIERID"
     gene            26196..26543
                     /locus_tag="VIG70_02240"
     CDS             26196..26543
                     /locus_tag="VIG70_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEY8249398.1"
                     /translation="MQLSPSVTCGLLLVASNVFMTFAWYAHLKNLAAKPWWIAALASW
                     GIAFFEYLLMVPANRIGYTELSLPQLKILQEAITLGVFVPFAVLYMNQPVKLDYLWAS
                     LCIMGAVYFIFRG"
     gene            26536..27168
                     /locus_tag="VIG70_02245"
     CDS             26536..27168
                     /locus_tag="VIG70_02245"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEY8249399.1"
                     /translation="MDKFRHLVVEGPIGAGKTSLARRLASRLGTDLVLERPEENPFLA
                     RFYDDMPRYALPTQLFFLFQRARMLEPLAQPDMFGRAVIADFLLDKDPLFARLTLSAD
                     ELALYEKIYAVLRLRSPPPDVVIYLQAQPAVLIERVRRRAARYERDISEDYLARLVEG
                     YARFFYHYDASPLLIVNSENLNFVERDPDFELLISRLRAMRSRREFFNLG"
     gene            27168..27965
                     /gene="panC"
                     /locus_tag="VIG70_02250"
     CDS             27168..27965
                     /gene="panC"
                     /locus_tag="VIG70_02250"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY8249400.1"
                     /translation="MQILRDIASLRRAVAAGTVAFVPTMGNLHAGHLSLVRQGRRHAP
                     AVAVSIFVNRLQFQPGEDFERYPRTFERDCELLESEGVEFLFAPDEKVLYPEKQQVRV
                     QAGPLGGELEGRFRPGFFDGVATVVLKLFNCVQPRVAVFGKKDYQQLMVVRALVRQLD
                     LPVAIEAAETVREPDGLAMSSRNSYLSPAERAEAPRLYRVVREVASGRSGPGAAVQLL
                     SKAGWKPDYVEVRRRGDLAVPRPGETGGVVLAAARLGQTRLIDNIEF"
     gene            complement(28007..28243)
                     /locus_tag="VIG70_02255"
     CDS             complement(28007..28243)
                     /locus_tag="VIG70_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249401.1"
                     /translation="MYAIVYKSDGFPICRQVPGVTPDPVVTWNTEAAAKAFISSKGHE
                     AEFQPVQLTDEAMDKLASTMGCPVEAMTFEPYPS"
     gene            28455..29396
                     /locus_tag="VIG70_02260"
     CDS             28455..29396
                     /locus_tag="VIG70_02260"
                     /inference="COORDINATES: protein motif:HMM:NF006452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY8249402.1"
                     /translation="MSNMNDAVGLCMPYSKTDSRYETAFEPAINTVWAYLNPKGVPCY
                     SLELLKAMIAHDKQLADNGGRVDFEGEMRKVDYYVTSSRTPGVFNLGGDLALFVLLIK
                     TGDREALAQYAKLCIDVQYPRTQSFFSPTLTSIALVQGDALGGGFECALASDVIIAEE
                     SAQLGFPEILFNLFPGMGACSLLERRIGMRAAEELILSGKMLPAYKLHEMGVIDVLAP
                     NGQGESAVRSYIADNAKRRNGMQAMFRARQFVRPVTREELDGIVELWVDAALRLGERE
                     MKMMSRLARAQARRMEHEHAPRPQDAAAMLEPLAMAS"
     gene            complement(29393..30331)
                     /locus_tag="VIG70_02265"
     CDS             complement(29393..30331)
                     /locus_tag="VIG70_02265"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8249403.1"
                     /translation="MGRAYFQRRAELPDAGEGRQLRLRAADGYEIGAVFYAALRPRAP
                     RRVAVLHGGAGIPALRYRRFATFLAEWGIPVLTYDYRGIGLSRPRALRGFQATIEDWA
                     EYDAAAAIAWVRERFPRDEILGLSHSIGCLPLGGAPNTAEQSRIVLVGAHTGYYGDYR
                     PLYRLPMAFAWHGLMPALTRFVGYFPADRLGLGEDLPAGMALEWAERRRPDLRPNGAE
                     PAGERRLRLLGHCAALQCPAIAISISDDAFATVDGTKRLLSYFPRLSSPELLLFTPPD
                     AQVRRIGHFGFFRRAVGTTLWPRLLAQLDPGRTGGA"
     gene            complement(30356..32893)
                     /locus_tag="VIG70_02270"
     CDS             complement(30356..32893)
                     /locus_tag="VIG70_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8249404.1"
                     /translation="MAEPSAPAAAAPNGLLAGLKARLKARPDTEHEQGVLRLVIGALL
                     VLYLLPGTTGTERELILWVGSIQFVVGVLIFLRIAYTTHISPSRRIFAQLADVATISC
                     YMAISGEWAAPLFLIYLWVTYGSGFRFGPKYLLTELVMSVVGFAIAIYLNPWWREHTA
                     LGVGLLLGMFFVSMYVLSLVRRMFEALARAEAANVAKRRFISVMSHEMRTPLNAIIGM
                     ADLLRDTTLTREQADMLQTLRGSSRVLLGLVEDVLDFSKIEAGKLALEKTDFDLHALV
                     NSTCRIVAAQAASKGVEFVVSIMPEVPPAVRGDPHHLRQIVINLAGNAVKFTERGSVT
                     VHVSAHSESDKSVRLKFSIRDTGIGIPAEAQARIFESFTQADQSTSRRFGGTGLGTTI
                     AKQLVELMGGKLGLESAVGLGSTFWFEIALEKQPERAGLAGAGDLAGARVLLVGFPQA
                     QREPLEQALAGWGVTPVAEATVEEGVARLVAEISLAKPYHSALLYASGEDPKLAQRFR
                     RAAPNPTPHTILAVPRDADVQRFDVLSSGFTAILELPFDKRQLFNVLHSVSAGEEMRE
                     GVVRLQDYARRGSAKKLRVLVADDNPTNREVIGKILERGGHSVTLANDGEQALDALQR
                     DAFDTVILDRNMPGVGGIEALQALRLMTRGRERLPVIILSADATPGTKREALEAGADA
                     FLPKPVEALKLLEEIQTLCAAAAERAQPAEAIPQKHGARGFEPVPIVNLETIDHLREL
                     GSSVAFVEKLIRVFIADNSTLLGRVEQALAARNYHELRSLLHAMKGSSASMGTDRLTR
                     LCSSYDKLSDAELRLQAPGLLRTLTEELATVRAQLERYVEERKSSAV"
     gene            complement(32880..33962)
                     /locus_tag="VIG70_02275"
     CDS             complement(32880..33962)
                     /locus_tag="VIG70_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8249405.1"
                     /translation="MAKAQVRVLPSARNTVMVVDDQSTGRAILEQVVRSLDERVVVEG
                     FARPVDAVVWATRNVSDLVLVDYMMPEMDGIELVKRLRAMPGYEHVPIVMVTVHDDRK
                     VRYAALDAGITDFLTKPVDARECLARCRNLLTLRRQQLALEDRRRLLEHMVEDATREV
                     KEREKETLLRLARAGEFRDEETGNHLIRMSRYSRLIAHALGLERDEAETIELAAPLHD
                     IGKIGIPDQILLKANALEKDEWQVMRRHPVIGHEILKGSASKYVRMGALIALGHHEKY
                     DGSGYPNGLVGDHVPLCARIVAVADVYDALTSVRPYKKAWPSEQAFEYVRTQAGRHFD
                     PRMVEAFLGAREQVLEIQHEWRDGRA"
     gene            complement(34063..34149)
                     /locus_tag="VIG70_02280"
     tRNA            complement(34063..34149)
                     /locus_tag="VIG70_02280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34113..34115),aa:Leu,seq:caa)
     gene            34181..35170
                     /gene="queA"
                     /locus_tag="VIG70_02285"
     CDS             34181..35170
                     /gene="queA"
                     /locus_tag="VIG70_02285"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY8249406.1"
                     /translation="MRVSEFDYELPTELIAQHPMPQRSASRLLRLDARTGSLQDLKFT
                     DLPQLVDEKDALVLNDTRVVKARLAGRKASGGKIELFVERALGEREALALIKASHPPA
                     PGSQVFIGDLPVTIESRDGELYRVRFSQPIESVLERFGAVPLPPYIRHAPLAEDAERY
                     QTVYAAVPGAVAAPTAGLHFDEGILNAVGARGATVAKITLHVGFGTFQPVRVDELEAH
                     RMHRERYRISSSTLQLIKDRRVLAVGTTALRALEAAHLSGKLEGDTDLFITPGFRFRV
                     VQRLLTNFHLPKSTLLMLVSAFAGLENIRKAYAHAIAERYRFFSYGDAMLIER"
     gene            35167..36261
                     /gene="tgt"
                     /locus_tag="VIG70_02290"
     CDS             35167..36261
                     /gene="tgt"
                     /locus_tag="VIG70_02290"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY8249407.1"
                     /translation="MNFKVTARDGAARRGVLELAHGSVDTPAFMPVGTYGTVKAMSPE
                     EVAGLGAQIVLGNTFHLWLRPGLEVIGKHGGLHRFMAWDKPILTDSGGFQVFSLGNLR
                     KISEEGVQFASPINGDRLFLTPEESMRIQRVLNSDIAMVFDECLAYPATEQQAAESMR
                     MSMRWAARSKKAWDSGNALFGIVQGGVYPALREESVRALKTIGFDGYAIGGLAVGEPK
                     EERSKILEGTKLPEDRPRYLMGVGTPEDIIEAVGAGLDMFDCVLPTRNARNGWLFTRF
                     GDIRIRNARYRDDTAPLDETCGCYTCRHFTRAYLYHLDKAKEILGARLNTLHNLHYYQ
                     ELMRELRGAIEQGMFLKKTRQLLEERRRLL"
     gene            36293..36616
                     /gene="yajC"
                     /locus_tag="VIG70_02295"
     CDS             36293..36616
                     /gene="yajC"
                     /locus_tag="VIG70_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY8249408.1"
                     /translation="MIISPAFAQAAPSGDSGYIGLLPIVLMFVLLYFLMIRPQMKRAK
                     ETKSMIEALQKGDEVITAGGVLGRITRISDAYISLEIAPNLEINVQKAAIQVLLPKGT
                     IKSIQ"
     gene            36613..38496
                     /gene="secD"
                     /locus_tag="VIG70_02300"
     CDS             36613..38496
                     /gene="secD"
                     /locus_tag="VIG70_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY8249409.1"
                     /translation="MNRYPTWGYALIGLAIALAFLYTLPNFFGESPAVQVSSARATVK
                     VDAALLQKVEDTLKKAGIPYRGAVFDPSGIRVRFDDTDTQYKAKDLIHAELNPGAAPQ
                     ATPEDDPKVSYVVALNLLPASPSWLSAIRALPMYLGLDLRGGVHFLLQVDMQGATTKR
                     LESIAGDIRSQLREKNVRHGGVTREGQTLRVRFRDAAMRNQAREVMLGNLPDLTITER
                     EDGQDLLLVATLKPEAVRRNQEYALKQNITTLHNRINELGVAEPIIQQQGAERIVVQL
                     PGVQDVARAKRLIGRTATLEIRMVAEEHTNPEALAAGARGQPPFGTEYYVERNGQPLL
                     VRRQVVLTGDRLTDAQPGFDNQTNEPAVHLRLDGQGARIFQEVTRENVNKRMAILLLE
                     KGKGEVITAPVIRSEIPGGRVQISGRMTTTEANDVALLLRAGSLAAPMEIIEERTVGP
                     SLGRDNIEKGFNATRWGFIAIVLFMSVYYVLFGVISSIALAVNVLLLIALLSLLQATL
                     TLPGIAAIALTLGMAIDANVLINERVREELRGGATPQAAIHAGYERAWATILDSNVTT
                     FIVGVSLLMVPGAVRGFAVVHCLGIMTSMFSAVVVSRAIVNLVYGHRRRVAHLSIGDT
                     AWK"
     gene            38508..39458
                     /gene="secF"
                     /locus_tag="VIG70_02305"
     CDS             38508..39458
                     /gene="secF"
                     /locus_tag="VIG70_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY8249410.1"
                     /translation="MEFFKIRRTIPFMRHALVFNIISLVTFLAAVGFLAFRGLNLSIE
                     FTGGTLIEVGYQRAADVDAIRRTLEGAGYKAEVQNFGSSRDVLIRMHLEKNQSSADLS
                     QKVVSILQEQDKSAELRRVEFVGPQIGSELVDYGALALLVTAVGIVLYLWMRFEWRFG
                     LSGIIANLHDVVIILGFFAFFQWEFSLPVLAAVLAVLGYSVNESVVVFDRVRENFRKM
                     RKATTAEVIDSAITGVISRTIITHLCTQMMVTTMLIFGGPALHYFALALTIGICFGIY
                     SSVLVASPLVMWLGARREQFIVPAKKKPGDRRDEHEGAVV"
     gene            complement(39525..41528)
                     /locus_tag="VIG70_02310"
     CDS             complement(39525..41528)
                     /locus_tag="VIG70_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY8249411.1"
                     /translation="MLYAGTIFLSSFLLFLVQPLIARLILPWFGGSAAVWTTCMLFFQ
                     ALLLAGYAYAHWLAKLSSKRLEAGIHTVLLLAAILTLPIAPGEAWKPAGDEEPVSRIL
                     LLLGASVGLPYFLLAATSPLLQAWFARARPGENPYRLFAVSNLASLVALVGYPFVVEP
                     FLGGAQQVGVWSWLFGVFAVLCAAMAWLTPRQAMEAATADETPTERSPYVLWLALAAT
                     GSVLLLAVTNHLTQNVASVPLLWLAPLTLYLASFIITFEGKGWYRPRWLWSLLLAWIG
                     AMGWLIVDDDFRFDLPLQLGIFLPGLFLGCLFCHGELYRLRPAPSRLTAFYLAVSAGG
                     ALGGLLVAVVAPLAFTGYYELGIALVALALLAAVRFANLNAFARYGSLAVLLGIAGCA
                     AYDGFRYQRDVLIASRSFYGVLRVKEYGLAGEESHLRRLVHGAIMHGEQYLHDKFRTM
                     LTTYYHEDSGIGAAIRSVGKRPARVGVIGLGTGTIAAYGRPGDVYRFYEIDARVISVA
                     QSEFTYLGDSKAKVELVLGDARLALEREAPQGFDVLAVDAFSSDAIPVHLITREALGV
                     YLKHVKPDGIVAFHVSNRFLDLIPVVARLAQEHGAHVALVRDDPDEAVDSRRSRTDWV
                     LVSRDAAALEREAIVERSPEAIEDKAEWRTWTDDYSNLIQILK"
     gene            complement(41528..42322)
                     /locus_tag="VIG70_02315"
     CDS             complement(41528..42322)
                     /locus_tag="VIG70_02315"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8249412.1"
                     /translation="MKLLAFLAVGFAACAGAQEIVTVQTRPGVTQSFFIANMGERKPQ
                     AAALLFAGGGGRINLRTEGGQIRFSQGNFLPRERREFIRNGILPLLLDNATDQKAGDG
                     MSDGFRASRPHAADIRAVVAEVKKRYPGLPVFLVGTSRGTVSVAYLAVALDGEVAGAV
                     LTSSLFYEGPRTNPRPVLAGFDWSAIKMPALLVHHADDSCFATPYRDAARLEKRYPLI
                     TVHGGKPAESGPCEPLAPHGFFGKEAETVDAIAAWMLGKPFAKEIR"
     gene            complement(42319..43683)
                     /gene="purB"
                     /locus_tag="VIG70_02320"
     CDS             complement(42319..43683)
                     /gene="purB"
                     /locus_tag="VIG70_02320"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY8249413.1"
                     /translation="MPSPLAALSPLDGRYSKGADPLRACFSEQALIRYRTRIELEWLQ
                     ALAAERAIKELKGFSPQARTAFASLVKNFSERDAEHIKNIEAETNHDVKAIELWLRSK
                     LAKNAEAQRALEFIHFACTSEDINNLAYALMIAESRAKVMLPKLDEVIGALKNLARKH
                     AALPMLSRTHGQAATPTTLGKEIANFAHRLARARERIAAVTALGKINGAVGNYNAHVT
                     AYPGFDWERFCRRFVERLGLEFNAYTTQIEPHDWLAELLDAYARANSVLLDLDRDLWG
                     YISLGYFRQKVKAGEVGSSTMPHKVNPIDFENSEGNVGIANALLRHLAEKLPVSRWQR
                     DLSDSTALRNVGTALGHTLLAYGACLRGLAKLEADPARLAADLEANWEVLAEAVQQVM
                     RRHGVPDAYDKLKAISRGKRLERRALAAFVKTLPIPPEEKKRLLALTPARYTGLAADL
                     AKRV"
     gene            43704..44285
                     /locus_tag="VIG70_02325"
     CDS             43704..44285
                     /locus_tag="VIG70_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEY8249414.1"
                     /translation="MRISLAVVSREARACRFCEDKLPQEPKPVFRAGKRATLLIVGQA
                     PGRLVHETGIPWNDPSGEVLRAWLGLDRDGFYDTSRIAIVPTGLCYPGTVGGADLPPC
                     AECAPLWQPRFRAALPEVRLTLLVGSYAQAYYLGSSRKKTLGETVRAYRDYLPEFFPL
                     PHPSWRNLLWLKRNPWFARSVIPALRRRVKACI"
     gene            44308..44925
                     /locus_tag="VIG70_02330"
     CDS             44308..44925
                     /locus_tag="VIG70_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8249415.1"
                     /translation="MKLLASPASPYTRKVRIVLTEKKIDCDLEVVDVNPAENPVNRHN
                     PLGKIPTLILDDGTVLYDSRVIVEFLDGKSPISRLIPEDLRDRVAVRRWEALADGVLD
                     AGLLVRYEAMRDASERSKAWSDKQLARVKRGMAQMTSELAERPWCHAERYSLADIALG
                     CCVGWLGFRKPGDVDWHAEYPPLARHYEKLMTRPAFSETVPQVPK"
     gene            complement(45004..46062)
                     /gene="mnmA"
                     /locus_tag="VIG70_02335"
     CDS             complement(45004..46062)
                     /gene="mnmA"
                     /locus_tag="VIG70_02335"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY8249416.1"
                     /translation="MKVVVGMSGGVDSAVAALLAKRAGHEVVGLFMKNWEDDDDDEYC
                     STREDLVDAAAAADTIGIELEAVNFSAEYKERVFAEFLREYAAGRTPNPDVLCNAEIK
                     FRAFLDHAMRLGAEKIATGHYARVVSEGNVFSLMRGKDNTKDQSYFLHRLTQEQLSRV
                     IFPVGGLKKTEVRRIALEAGLPNHAKKDSTGICFIGERPFREFLNRYLPKAPGAIVDD
                     KGKRVGEHMGLAFYTIGQRKGIGIGGAGEAWYVADKRIAANELVVVQGHDHPLLMKKT
                     LSAADTAWVSGNAPATASAHTAKTRYRQADAPCTLSKVLESEVRVDFSSPQWAVTPGQ
                     SVVLYDGEICLGGGVINS"
     gene            complement(46059..46508)
                     /locus_tag="VIG70_02340"
     CDS             complement(46059..46508)
                     /locus_tag="VIG70_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8249417.1"
                     /translation="MAIWKPSVTVAAVIERDGKFLLVQERISGRLVLNQPAGHLDPGE
                     SLAAACSREVLEETAHRFEPTALVGIYRWRDPAKDFTFLRFCYRGTLAGTVERPLDKE
                     IVALHWLTPEELRQRKAEHRSPLVQKCVDDFLAGRRFPLDVFSPEFS"
     gene            complement(46524..47738)
                     /locus_tag="VIG70_02345"
     CDS             complement(46524..47738)
                     /locus_tag="VIG70_02345"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY8249418.1"
                     /translation="MPEISRRANSLGTENAFVVLAEVNALARQGRDIVSFCIGQPDFP
                     APHNVQEAAIRAIKGGKHGYTPSAGIDELREAAAKYLAKMRGGLPIRAEDVVVGAGAK
                     PFIAYAILSTTDHGAGDEVIYPNPGFPIYESQIVANGAKPVPIHLHENRNFSFDPAEL
                     ERLITKKTRLLILNYPHNPTGGCLTRNELETIAGVLKKHPQVWVYADEIYSRLVFQSE
                     FFSIAQVPGMYERTIISDGASKTWAMTGWRIGFTSNPQLAPVFTRWITNTESCASQIS
                     QWAAVEAITGPQEAAAAMKKSFLERRDFIVGLLNEVPGVKCQIPGGAFYVWPNVTEAC
                     RRIGAADSEEFRKRLLHEAGVAVLADIHFGTRVPGEGQHLRFSYAASKQAIEAGVQRM
                     SDFIRKNTKKAA"
     gene            47762..48730
                     /gene="corA"
                     /locus_tag="VIG70_02350"
     CDS             47762..48730
                     /gene="corA"
                     /locus_tag="VIG70_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEY8249419.1"
                     /translation="MLVNCVAYEGGRKLADIEKREISNYLARPGCFVWVALRDATEAE
                     LAEMQEEFDLHPLAIEDARVGHQRPKIEEYGDSLFVVMHTIEVEGDDLRAGEVHVFAG
                     ANYVLSVRHRTERGFQDVRARAEREPDLLSHGSGYVLYALMDAVVDRYFPVLDAVEME
                     LEKLEGRLFTPGASPRENIESLYYVKQKLTTMKHATAPLQENAGKLYGGRVPGVCHGL
                     GEYFRDVYDHLVRINQSIDAARETVNTAIQVALAMVATGQGEITRRLAAYAALVAVPT
                     MIAGVYGMNFEHMPELQWELGYPLTIAAMVVLDVVLFWRFRKAGWV"
     gene            complement(48738..50078)
                     /locus_tag="VIG70_02355"
     CDS             complement(48738..50078)
                     /locus_tag="VIG70_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8249420.1"
                     /translation="MNPRDLSAASALNLEHYWMPFTDNRRFKAKPKLVTAAKDMHFTT
                     AEGRQVLDGLATLWCVNAGHARPRIVEAIRRQAGELDFAASFSLGHPLAFRLAERIAA
                     LAPKGMGHVFFTNSGSEAIDTAMKIAIGYHRLRGEGTRTRFVGRERSYHGVNIAGMSL
                     GGVPANRKAYSGALLPGIDHLRHTHEIEGNSFSRGEPPNGVELANELERIVTLHDASN
                     IAAVVVDPVSASGGVLVPPKGYLKRLREICTRHGILLVFDEVITGWGRLGAPFAAQHF
                     GIEPDLITYAKGITSGAVPLGGVIVKDSIYEAFTSGKVPGVEFFHGYTYSGHPLACAA
                     GIAALDTYEEEKLFEKPRTLGKYFEDGIHSLKGLPNVVDCRNLGLIGAVELAPRPGAA
                     SGVRAQELFEKCWDKGVFVRPVGDSLAFCPPLIAEKKHLDQMFGTVAGVLKETP"
     gene            complement(50101..50469)
                     /locus_tag="VIG70_02360"
     CDS             complement(50101..50469)
                     /locus_tag="VIG70_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8249421.1"
                     /translation="MTSSAKSRKLEIRIGAAADALDRFEAAWNRVAEGRAMRPLDILS
                     FSDLPLLLRTLSPARWTLLQKLHADGAMSIYELARRLQRDYKNVHTDVTQLARIGLVE
                     RRADALAAVAWNLLRAELRL"
     gene            complement(50438..50617)
                     /locus_tag="VIG70_02365"
     CDS             complement(50438..50617)
                     /locus_tag="VIG70_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249422.1"
                     /translation="MLLLRETVVLEGSILEIELHDDLRYRLRWRGVTYDSRRKYDFRS
                     VEQLRYDFEREVQKA"
     gene            50660..52636
                     /locus_tag="VIG70_02370"
     CDS             50660..52636
                     /locus_tag="VIG70_02370"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEY8249423.1"
                     /translation="MKDFFSLYRHGFARVAAATPLVRVGDPQYNLDATVELMREAARE
                     KAVLAVFPELGLSAYTCDDLFHQQALIEGAEEALAALIKSSKNLPIAALVGLPVAVDG
                     LLYNCAALVCQGRLVGVVPKIYLPNYREFYEGRQFTPGDTARRSEIRLAGQVCAFGTD
                     LLFKAAELPKLVLHVEICEDLWVPAPPSSFAALAGATVIANLSASNVVIGKEGYRHQL
                     VGNQSARCLAAYLYSAAGLGESTTDLAWDGHAVIYENGTLLAESRRFADAPQLAVGDV
                     DLDRLLADRMRQNTFGASMRRHADRAFRTVEFSLPLPKGNVALERKIERLPYVPGDPK
                     TRDARCEEVYRIQVQGLVTRMRATGTKKLVIGVSGGLDSTQALIVCARAMDELKLPRA
                     NILAYTMPGFATSTRTRSQAWQLMRAIGATAEEIDIKPSCMQMLKDIGHPFAKGKKVY
                     DVTFENVQAGERTSHLFRLANFHGGMVVGTGDLSELALGWATYGVGDHMSHYNVNASV
                     PKTLIQFLVGWVGESNEFSADVKRALRRVLETEISPELVPGRQSSEATVGPYELQDFN
                     LYYILRFGYSPAKVAFLAWHAWRGKYRLPEIRKWLGVFLKRFFQNQFKRSAVPNAPKV
                     GSGGSLSPRGDWRAPSDGSANAWLKDLARVPTAE"
     gene            52739..52945
                     /locus_tag="VIG70_02375"
     CDS             52739..52945
                     /locus_tag="VIG70_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249424.1"
                     /translation="MKPHSSNLEALLNTAPSAFAGGDLPVLPRIKSLAALRVEVRRHF
                     GKLSNQELDALAAWFNRNFFRLER"
     gene            complement(52953..53129)
                     /locus_tag="VIG70_02380"
     CDS             complement(52953..53129)
                     /locus_tag="VIG70_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249425.1"
                     /translation="MKRKYGAALARETLAEQRMRERAAEMKDNSKNYGSKAAKGSPVR
                     GKRRGRAYKPAYMK"
     gene            complement(53137..>53701)
                     /gene="lpoB"
                     /locus_tag="VIG70_02385"
     CDS             complement(53137..>53701)
                     /gene="lpoB"
                     /locus_tag="VIG70_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HEY8249426.1"
                     /translation="ALGALAAILALAAGCAQEKTRYGDAKGVETLTNQYGSTDLQMMA
                     ESMARSMQQAPAIASANLPILTVQEVRNKTSEYIDTRAITDSIRSELQKGGRVRFAVD
                     AVGMGQQTEELKRQQSELYAKDSAAEMGRMVGAQYRLEGNIISIVKQVKDTKDVYYKL
                     NLQLWNIRNGLLEWSDEKEIRKTTTRG"
BASE COUNT         8678 a        18377 c        17972 g         8674 t
ORIGIN      
        1 cagggtcatt tctgtctccg gcgcagcttt tcctcgcgct cgaggatacc gcgttcgatt
       61 gcgcgcagcg cttccagttg ctgcttgagc gcttcctcgc gcttgtcgcg ctccttgtcc
      121 aggcggtcgc gctccttgtc cacgcgatcg cgctccttgt ccaggcgatc gcgctctctc
      181 gcctcgcgcg cgaggtggtc gagctcgcgg gcaattcgct gccgctcgac gatctgcgag
      241 gagagcaggg cggcgaagcg cccgaccccc ggcgaggcgg cgtccgccag cggcccgagg
      301 agctcgagcg cacgcgcatc gtcgcgcagc ggcgggggca gcgtggcgag gaacgtggcg
      361 agtcgcagcc agtcgagggg gttggtgccg gcgctgcgtg cctgcttctc cgcccgcgcg
      421 agcgccgccg cctgctcggc cgctggagcg cgcgcggtgg tgatcgcctc gccgaccacg
      481 gcgtccaccg ggttgatcag gggttgcacc gaggcacaac ccgcgaggac gaggctcagg
      541 aaaacgaaac gcgcgctcat gccgccattc ggcggggcag cgcgacacgg aaatgcgcgc
      601 cgcgctcctc ggcaagcacg ctgatcttgc cgccgtgggc ctcgacgaac tcgcgcgcga
      661 tggcgaggcc gagcccgctg ccttcgacac gcgcgctcgc cttggcgcgg ccgcggaaga
      721 acgagtcgaa gatcgagtcg cgctcttccg ccggcacccc gggcccggaa tcgattacgt
      781 cgatcacggc ctcgtcgccg ctcgagcgcg ccagcaccgc gacgctgccc cccgcggggg
      841 tgaacttcag cgcgttgccg atcaggttgt cgacgatcga gcgcagcttc tcggggtcgg
      901 cattcacctg cacccgctcg agctcgaggg cgagccgctg ccccttcgcc tgcgcggcga
      961 gctcgtgccc gcgcgccacc tcgcgcacca gcgcgtcgag cgcgaggcgc cgcggcgcga
     1021 gcgccgccgc cgcgtgcagc gcgcgctggt agtcgagcag ctcctcgatc aggcgctgca
     1081 gcttgatgct gttgtccttc atgatcgaca ccacccggcg ctgcgagccg gacagagggc
     1141 cgccgacctc gtcgtgcagc agctcggccc cttcgcgcag cgccgtgagc ggcgtcttca
     1201 gctcgtgcga caggtggcgc aggaagcggt tcttctcgtc ctcgagctcg accaggcgcc
     1261 ggcgcagcca gtcgaggcgc tcgccgagcg tcttcaggtc ctcggggccg cgcaccgaga
     1321 tcggctgcgc gaaatcggcg ccgccgagct gccggatcgc ggcgtcgagc tcggcgatcg
     1381 gccgggcgat gaaccgcgtc agcgcaagcg cgagcgccag cgcaaccgcg gtgctgaaga
     1441 tcaccagcag cacgagctgg cgctgcaccg actcggcctc ggcgcgcagg cgcagcacct
     1501 cgcggtcggc gacccgcgtg ctgatcgcga gcatctcgga agcgttctcg gccaggtcgg
     1561 cggcaagcgc cgcgacttgg cggcgctcgg cgacggcgcg tggaccgctt cgcggcgcgg
     1621 ccagcaggtc gtagaggccc tgctcctgcg ccatcgtctt cttgagcgcg ccgagctggt
     1681 cctcctcgag gggcagctgc agcagctcct gcgccaccag cacgaagctg gtatgcaccc
     1741 ggccgaggtc cgaatcgagc cccggcgcgt tgagcatcgc gagccgctcg agcgagctga
     1801 tgcggttgac gagggtgcgg ctggcgcgcg ccgcctgcga ggcattgaac acggcgtgcc
     1861 ggctctgctc ggcgaggcgc tcgctgtgcc acgcggaata ggcgagcgcg gcgatcaacg
     1921 gcgccgccac cagcacgaag ctggcgaggc acagcccgag aaacgatcgc ggatacgcca
     1981 tgtctttata ctttcaggca aatattgccc ttagcgagga cgaatgtcac aagcctccgg
     2041 cccgcggatt tacctggtcg ccgcggtagc tgcgaatgga atcataggcg caaagggcaa
     2101 gctaccctgg catattcccg aggagctgaa gcacttcaag cggctcaccc tcgggcatcc
     2161 ggtcatcatg ggccggcgca cctgggaatc gctgaaaggg ccgctgcctc agcgcgagaa
     2221 catcgtggtc acccggaccc ccggctacga agcgcccggc gcggcggtgg cgagctccct
     2281 cggcgcggcg ctagccctgt gcatcggcga gccggtggcg ttcgtgatcg gcggcacgca
     2341 gctcttcgag gaggcgctgc cgcacgccgc cggcatggtg ctcaccgaga tccagcgcga
     2401 ctacgagggc gatacctggt ttcccgcatg ggaccgcgcc caatggcgcg agtcgcagcg
     2461 cgaagcgcat gccgcggtgg acggtacgcg cttcgatttc gtgctgtacg agaagaaacc
     2521 ctagcttctg ctctcgaaaa cccgcacttc gggcagcggg aagccgttga actggctcgc
     2581 gtaggccgtg gtgtaggcgc cggcgttctg gatgtagatg aagtcgcctt cctgcaggtc
     2641 ggagggcagc ggctcctccc gcatgacgac gtccaccgag tcgcaggtcg ggccgcccac
     2701 ggtccaggcg acgtccggcc ccgagcggtc ggtgcgcacc cggtacctca gcccttcgct
     2761 cgactcgatt acgccgccga agaggcccgc gtcccagtgc atccagcgct tgcccgcgcg
     2821 cgtcgctgtg ccgagcaccc ggcagacgaa gtagccggcg tcggacacga ggtagcggcc
     2881 gggctcggcg atcacctgca cgtcctccgg gaaggccttc aggccctcgt tcaccacctc
     2941 gccgatgacc tcgatcgagg ggatcggctt gacgtggcgc accgggaaac cgccgccgat
     3001 gttgagcagg cgcggcttga ggcccgcctt gcccatggcg tcgaacaggg ccttcgcctt
     3061 ctcgagcgca acgcgccagt tctcggggtt gcggcactgc gagccgacgt ggaaggtgac
     3121 cccggcaagg tcgacgccca ttttcgccgc cgccgccacg atctcgcgcg cgtcggcgac
     3181 gccgcagccg aacttgcccg agagcggcca gtcgctgccg atgttcgggg tcgcgatgcg
     3241 caggtactgc ttggcgtccg gcttgacttc atgaacgcgc cgcagctcgt ccacgctgtc
     3301 gaccacgtac cactccaccc ccttggccgc ggcgtaggcg atcgcctcgc gcgacttcat
     3361 cgggttggag tagaagactt ccgccgccgg cacgcccagc ccgagcagca ggtcgagctc
     3421 ggccgtcgag gcgatctcga agcccgcgcc ctcctgggcc agcaccttga gcacccggcg
     3481 gtcggggttc gccttgaccg cgtagtgcgg gcgcacgcgc ggcatggccg cgcggaagcg
     3541 ccggatcttc gatcgcacga tggcggtgtc gaggatcagg aacggccggg tgtacttctg
     3601 gtgtgccgcc tcgcgcacga gctcgaagtc gaggctgacc tcggcgtgcg tgtcgaaacg
     3661 agcttcctgc gactgttgct gcttgagtac tgcggcggat tgtctgtgtg tgatgctact
     3721 actgcggtcc cccatcaaag tctctcctct gtcacgaact aagtttcagg aatgtcccga
     3781 acgacgacgt cgtcggccgg acgcgagttc cttgtgcttg tcatgatgac ctccttctct
     3841 tcgttgaatc caaaaagaaa aaaacttgag cggagtatag ggacatgacc ccgcctgtca
     3901 agacctccgc gaggattttt ttggcttttt acgggaacta gatgcgcggc aaggtcacgc
     3961 ctttttgacc ttggtatttg ccgccgcggt cgcggtagga cgtttcgcaa acctcgtcgg
     4021 actccaggaa gatcacttgc gccacgccct cgttggcgta cacgcgcgcc ggaagcggcg
     4081 tggtgttcga gaactcgagc gtgacgtgcc cttcccactc cggctcgagc ggcgtcacgt
     4141 tgacgatgat gccgcagcgc gcgtaagtgg acttgcccag gcagatcgtg agcacgctgc
     4201 gcgggatgcg gaagtactcg acggtgcgcg cgagcgcgaa ggaattgggc gggatgatgc
     4261 acacctcgcc gcggtagtcg acgaagttct tctcgtcgaa gtgcttcggg tcgacgatgg
     4321 tcgagttgat gttggtgaag atcttgaact cggtcgagca gcgcacgtcg tagccgtagc
     4381 tcgaggtgcc gtaggaaacg attttcttgc cctcgaacgc cttgacctgg ccggcctcga
     4441 aaggctcgat catccccttg gccgccatgc ggcggatcca tttgtccgat ttgatggtca
     4501 cgggtctatg atagccgctc aagatgcgga ccatcgccgt cctggcggcc ctgctgagct
     4561 ccggcgcatt cgccgcggat gcgcttttca ctccgggcga aatccgcgcg atccttgccc
     4621 acggcccgtg gccggcgccg gcgacgagcg atccgagcaa ccgggtctcg ggccggcgcg
     4681 acgcgatcga gctcgggcag cgcctcttct tcgacccgcg cctgtccgtc aacggcaagt
     4741 tctcgtgcgg cacctgccac gtgcctgagc gcaactggac cgacaaccgc gtgcgcggcg
     4801 tcgccgccgc ggaagtcgac cgcaacacgc ccaccctgat gaacgtgcgc cttggccagt
     4861 ggtacggctg ggacggcggc gccgacagcc tgtggtcgca aagcatccgg ccgatcctcg
     4921 accggcgcga gctgggcgcc accccgcggc acgttgcgga cctgatgcgg caggacgagg
     4981 atctgtcgtg ccgctatcgc cgcgccttcg gcgccgcgcc ctcgcccacg gacgacgaag
     5041 aggtcctggt caacgtgggc aagatcctgg ccgcgttcca ggaaaccttc gagaccccgc
     5101 cgacgccctt cgaccgcttc cgcaatgcgc tggcgcgcgg cgagcccctc aaagcggggc
     5161 tctattccga ggcggcccag cggggactga agatctttgt cggcaagggc aattgctcga
     5221 gctgccacag cggaccgaac ttcaccagcg gcgagttccg gaacaacggc ttctccgcgg
     5281 acgccgggcg ggtcgaggga ctgaaggcgg tgaaggccag ccgcttcaac ctggccggac
     5341 cctacaacga cgacccgcta aggcgcgtgc acgctgctca ggaacccgag gcaggagcct
     5401 tcaaggtgcc cacgctgcgc cacttgatgc tgaccgcccc ctacggccac gacggcaagc
     5461 gcgaaacgct ggccgaggtg gtgcgccact actcggaaaa ggggtcgacg gccttgccgg
     5521 cgctgaagct gactgcggcc gagcagaccg acctcgtggt gtttctcgag tcggtcagta
     5581 ccctcagcaa tccctggcgc cccgaggacc acgctcgctg ccagtagctg tgtcgctctc
     5641 cgttcgcccc ctggccccga atctcggcgc ggtgatctcc ggcaccgatc tttcctgcga
     5701 gctcgatgat cgggtattcc gcgagctgta ccaggcgttt ctgcgctacc aggtgctgct
     5761 ctttccgccg caggatctgc cgcccgccag ccaggtcgcc ttcgcccgcc gcttcggcga
     5821 agtgcaggtc cacgtgatga accagtacca cgccgacggc tacccggagc tctaccggct
     5881 ctcgaatctc gacgagaacg gcaagccgca cggccgccac ccggacaagg gcacgcttgc
     5941 gtggcacacg gacggctcat ggcagcgcgt gaccgggcaa gccacgatca tctacggcga
     6001 ggtgatgccg caaagcgggg gtgagacgca tttctgcgac atgtacggcg catacgaacg
     6061 cctcgacgcg tcgtggaagg cgcgcatcgc caacctgcgc gccgtgcaca acctggactt
     6121 ctcgcgcacg cggcgccacg gcgaggatcc gctgaccgag gcgcagcgcc tggcgaagcc
     6181 cccggtcgac caccccgtcg tgcgcacgca tccggaaacg gggcgcaagt gcctgtacct
     6241 cggcgatcac gcggaataca tccagggcat gccctacgac gaaggccgcg cgctgatcga
     6301 ggagctgaac gcgcttgccg tccatccgga cctcacctac gagcaccgct ggaagcctcg
     6361 cgagctcctc gtgtgggaca accgatgcgt gatgcaccgt gcgactgcct acgacgccgc
     6421 cacgcagggc cgcgtgatca ggcgctgcac ggtgctcggc gaggttccgg cttgaattcg
     6481 gggacggagc cctaactcca cggctgcgca gctttcggag ttagggctcc gtccccgaat
     6541 tcaggtgttc tggacgacga tgttcgggaa cttggaagtc atgtcgcgct gcgactcggc
     6601 gatcttgacc gcgcagcgcc gcgcgattcg ccggtagatc gccgccagct cgccgtccgg
     6661 gtccgacacc acggtgggct tgcccgagtc ggtctggctg cggatcgact cgtcgagcgg
     6721 caactggccg agaagctccg tcttgtagtc cttcgccatg cgctcggcgc cgcccgagcc
     6781 gaagatgtgg ctctcgtggc cgcacttggg gcaggtgtgg aaagacatgt tctcgaccac
     6841 gccgagaatc ggaatcccga ccttctcgaa catcttgagg cccttgcgcg cgtcgatgag
     6901 cgcgatgtcc tggggcgtgg tcacgatcac cgcgccggtg accgggactt tctgggcgag
     6961 cgtgagctgg atgtcaccgg ttcccggcgg caggtcgacc accaggtagt cgaggtcgcg
     7021 ccagttggtg tccttgagca gctgctcgag cgcctgcgtc accatcgggc cgcgccacac
     7081 catcggcgtg tcgatgtcga tgagaaagcc gatcgagctc gcctgcaggc cgtggccctc
     7141 catcggctcg atgcgcttgc cgtccttcga ctcgggacga ccggcgatgc cgagcatcat
     7201 cggctgcgag ggcccgtaga tgtcggcatc gagcacgccg accgaggcgc cttccgcggc
     7261 aagcgcaagc gcgaggttca cggcggtggt cgacttgccg acgccgccct tgcccgaggc
     7321 gacggcaatg atgttcttga ttcccggaac cagcttcacg ccgcgctgca cggcgtgcga
     7381 caccaccttc gacgacacct tcaccgacac gttggccgcg cccacgctct tcagcgcctg
     7441 cgtcacctgg cggcggatcg gctcgtgctg gctcttgccc ggatagccga gctcgatctc
     7501 gagcgcgaca tcgctccccg agaccttgat gttgcgcgcc gagcggctgg ctacgtagtc
     7561 cttgccggtg ttgggatcga cgagctggcg gagcgcttct tgaacgctgc cttcattcac
     7621 ggacatgatt gaagtgtaag ctaaaatcgt cgccctccct catgattagc cacgcaaaag
     7681 cgcgcaagct cttcgtcacc accgctttgc cgtacgcgaa cggcccgttc cacatcggcc
     7741 acatcatgga atacatccag gccgatatct gggtccgttt ccagcgcatg caaggacacg
     7801 aggtgcattt cgtcggcgcc gacgattcgc acggcgcgcc gatcatgctc gccgccgaga
     7861 aagccggaaa gtcgcccgac gccttcatcg aggcgatcca gaagggccgc gagccctatc
     7921 tggagggctt tcacatcagc tacgacaact ggcattcgac ccactcgccg gaaaacaccg
     7981 agctgtcgca ggacatctac cgcaagctga aagcggccgg cctggtctac gccaagcccg
     8041 tcgagcagtt cttcgacccg gtgaagcgca tgttcctcgc cgaccgctat atcaaggggg
     8101 aatgcccgaa ctgcggcgcg aaagaccagt acggcgacgc ctgcgagaac tgcagcaagg
     8161 cctactccgc caccgagctg aagaatccct attccgcgct ctcgggcgcg aagccggtgc
     8221 tcaagtcatc ggagcatcac ttcttcaagc tctccgaccc gaaatgcaag agcttcgtcc
     8281 aggagtggct ggaaaagccc ggacgcctgc aatctcaggt ggtgaacaag gcaaaggagt
     8341 ggctcgaagg cactggcgac aaggcgctcg ccgactggga catctcgcgc gacccgccct
     8401 acttcggcat ccgcgtcccc gacatccagg aagagaagta cctctacgtg tggctggacg
     8461 cgcccatcgg ctacctcgct tcgttcaaga actattgcgg caaggccggg ctcgatttcg
     8521 agaggttcct gcaggatccg aaaaccgagc agatccactt cataggcaag gacatcatct
     8581 acttccacac cctgttctgg ccggcgatgc tgaaattcgc cggcaagccc tacaaggtgc
     8641 ccgaccacgt ctacgtgcac ggcttcatca ccgtgtcggg cgacaagatg tccaagtcgc
     8701 gcggcaccgg gatcgacccg ttgcgcttcc tgaagctggg catgaacccc gagtggctgc
     8761 gctactacat cgcggccaag ctcaacgcca acgtcgagga cctcgatttc aaccccgacg
     8821 atttcctcac gcgggtgaac gccgacctgg tcgggaagta cgtgaacatc gcggcgcgcg
     8881 cggcgagctt catctcccgg cagttcgacg gcgcgctgca tccatcgaat gatgtaagcc
     8941 tcgccctgac ctcggccagg gcgattgccg aagcctacga gcagcgcgag ttcggaagag
     9001 tgatccgcga agcgatgcgg atcgccgacc tcgtcaacca gcgctacgat cagctcaagc
     9061 cttgggagct cgccaaggac ccggcgaaac gcgaggagct gcaccgcgtc tgcagcgacg
     9121 cgatccgcgc cttcttcgtg ctcacggtgt atctcgcgcc gatcctgccc gccaccgcgt
     9181 cgcgtgtcgc gcgcgagctg ttcggcttca agcgcgattt cacctgggct gacgcgagcg
     9241 cgcttccgga ccgcatcgcg ccgttcaagc atctgatcac ccgcgtcgag gaggcgcagc
     9301 tcgacgcgct gttcgagccg ccccaggaga aaactgtgga gcagacgacc ctttcgatcg
     9361 acgacttcaa caagctcgac ctgcgcgtgg cgcgcatcgc gcgcgccgag caggtcgagg
     9421 gcgccgacaa gctggtcaag ctgaccctgg aggtcggcga cggcacgcgc accgtgttcg
     9481 ccgggatcaa atccgactac gcgccggaga agctcgaggg ccgcctcgcg gtgctggtgg
     9541 cgaatctcgc gccgcgcaag atgaagttcg gcgtctcgga agggatggtg ctcgcggcct
     9601 cgggggacgg accgggcatt tttctcgttt cccccgatgc cggggcccgg ccgggcatgc
     9661 gcgtaaaatg agccgctacg gctcgagagc cgcttaaggg ggaggcccgt tggacagcgc
     9721 tggccgaacg ctggtctgca gcgtcctgtt tctggacatc gccgactact cgcagaagcc
     9781 ggtcgccgag cagctgcagc tcaagcaggc cttcaatcag gccctggggg tggcgctcga
     9841 gcacgtgttg tcgcgcgatc gcatcatcct cgataccggc gacggcgcgg cgatcacgtt
     9901 catgggcgat ccagaggatg cgctgttcgc ttcgatggcg atgcgcgatc tcgcgaccag
     9961 cgtgccggtg cgcagcggcg tgaatctcgg cccggtgcgg ctggtgaagg acctgaacgg
    10021 gcagatgaac atcatcggcg acggcatcaa cgtcgcgcag cgcgtgatga gcttctcgcg
    10081 gcccgggcag ctcctggtct cgcgctcttt ctacgaggtc gtgtcctgcc tgtcgcgcga
    10141 ctacgcgagc ctgttccgcc acgagggctc gcgcaccgac aagcacgtgc gcgcgcacga
    10201 ggtctacgcc gtggtcggcg ccacgcccgc gccgcggcgc gtggacgaag ccgaagcccg
    10261 cagcgaaagc acgccgccgg gcgcggtcgg ctggctttca ggcatgggcc cgttcggcct
    10321 gcgccgctcc gcgctcgccg cagcgccgct cgcttttctc ctgatcgtcg ccggcggggg
    10381 cgtcgcccgt gcgctgatgc ccgacgagcc cgtggtcaaa ccgcaaccgc cgaggacggg
    10441 cgccaagccc gctgcgccgg cggctcccgc ggcgggaagt gcggctccag cggcgggagg
    10501 cgcggctccc attgccggcg gcggcgcggc ggtgatcgag gagccgccca agcagaagaa
    10561 gcagatcgtc gcgcgcgcgg agaagaaacc cgagaagccc gcggccgcga agggtccagg
    10621 gcggctcgag ctcgccgtcg cgccctgggg cgaggtgctg gtcgacggca agagccgcgg
    10681 cctgagcccg ccgctgcgcg tgctcgaagt cacgcccggt ccgcatacga tcgagatccg
    10741 caattccacc ttccctgcgc acgtcgcgcg cgtcgaggtg aagcccgggg aatcggtgaa
    10801 gatccagcac cggttccgtt gatgggacgg gcgctcttcc tcctgctgtt catcgtcctg
    10861 gcgggttgcg cccagttgga tcagctggtg gtggccacca aggcccggct caccctggtc
    10921 gaggggctgc gccagttcga cgccggcgat cacgtcacct cggcgaagac cctggcaagc
    10981 gcgctcgaga tgggattgag cgacaaggac cgcgccaacg cgcacaagca tctcgccttc
    11041 atccattgcg cggccggccg cgagcgccag tgccgcgagg agttccgcaa ggcgctcgcc
    11101 gcggatccgg caacccggct cgacgcagcc gaagccggcc atccggtgtg gggaccgatc
    11161 ttccgcaccc tgagcggcag cgccgcgctc gaggccgggc tgaagcagta cgagagcggc
    11221 gaatacgcgg aagcggcgaa gaacctgcag ggggcggtcg acctcggcgt ctcggacaag
    11281 cagcgcgcca acgcccacaa gcatctcgcc ttcatccact gcacggccaa ccgcgagcgc
    11341 cagtgccgcg aggagttccg caaggcgctc gccgccgacc cggcgctgca gctcgatgcc
    11401 gcggaggccg gccacccggt gtgggggccg atcttccgct cgatgaacgg cccttcggcg
    11461 ctcagcgccg gcctcaagct gtacgagagc ggcaactacg aggaatcggc gaagaacctg
    11521 caggtggcga tcgagctcgg ggtgccggag aagctgcgcg ctaacgcgca caagcatctc
    11581 gcgttcatcc attgctcgtc gaaccgagag cgccagtgcc gcgatgagtt tcgcaaggcg
    11641 ctcgccgccg acccgtcgac ggacctcgac gcggcggagg ccgggcaccc ggtgtggggc
    11701 ccgatcttcc ggtcgctgaa gggggcggcc aaatagttcc ctaagcgggt cggttaagct
    11761 tgcggcatgg agcaacccca gctcggccgc tacgtgatcg attccgagat cggccgcggc
    11821 gccatgggcg tggtgttcaa ggcgaccgac acggtattgc agcgcacggt cgcggtgaag
    11881 accgtcaaca tggcgctgga gaaggaccac gccgacaagt acgaggcgcg cttctaccag
    11941 gaggcgcgcg ccgccggcgg gctgaaccac cccaacatcg tcacggtgta cgacgccggc
    12001 aaggcgggcg acgtggtgta catggcgatg gagtacatcc agggggtgga gctgcgcacg
    12061 ctgttcgtcg aaggccagcc gatgggcgtg ccgcaggcgc tgtcgatcgc cgcccaggtc
    12121 gccgaaggcc tcggctacgc gcaccagcag ggcgtcgtgc accgcgacgt caagcccgcc
    12181 aacatcatgg tggtttccga gggcccggtg aagatcaccg acttcggcat cgcgcgcatg
    12241 cgcgcctcgg ccgatctcac gcagaccggc gtgatgctcg gctcgccgaa gtacatgtcg
    12301 cccgagcagg tgatcggcaa gcgcgccgac caccgctcgg acatcttctc gctcggcgtg
    12361 atcctctacg agatgctcac cggcgcggcg cccttcagcg gcgagaacgt caccgcgctc
    12421 atgtaccaga tcgtcaactt cgccccgcct gcgccgagca ccgtcaaccg ccaggtgccc
    12481 gagatgctcg acttcgtggt ggcgaagatg ctcgccaagc cgctggacga gcgctatcag
    12541 gacgcgcgcg aggcggcgcg cgacctgcgc gagtgcgagc gcagcctcgc cggcccggcg
    12601 tcgaccacgc tgtcggggcg ccccctcggc ctcgcgagcg gcacgcagcc ggagatgatg
    12661 agcacgcaat ccaaggccgt ggtgctcgcc caatcggtga accggacgcg ccagctggac
    12721 gaaaagacgc cggcggaggc gggagcgccg ctcgcgcgcg gcgtatcgaa ttcgttcgat
    12781 tcgaacgagg ccacgcagcg gctcgccgcg ctcacgggat cggccacgat tccgcagacc
    12841 gccacgcagg cgatcggcgc ggtgccgcac ccgccgcgcg ggcgctggcg ccggcgcgac
    12901 tggctggtcg tcggcggcgc aacgctggcc gggctgatcg tggccggact catcgcgcgg
    12961 cggtgaggga tctgcaggcg cttttccgga acaaccggcg ctgggccgag cgcatcctgc
    13021 gcgccgatcg gcgcttcttc gacaagctgg cgcgccagca gtcgccacgc tacctgtgga
    13081 tcggctgctc cgacagccgc gtgccggcga acgagatcgt cggccttctc cccggagagc
    13141 tattcgtcca tcgcaacatc gccaacatgg tcgtgcacag cgacctgaac tgcctttcgg
    13201 tcatccagtt cggcgtcgag gtgctcaagg tgcgccacat catcgtctgc gggcactatg
    13261 gctgcagcgg cgtgcgcgcg gcggtgatga aggagcgcca cggcctggtg gacaactggc
    13321 tgcgccacat ccaggacgtc cggcacgagc acgcgccgcg cctgcgcgcg gtgcgcgcgc
    13381 gcgcgcggct cgtcgaccgg ctgtgcgagc tgaacgtgat cgagcaggtc gccaacgtct
    13441 gccgcacgtc gatcgtcgag gactgctggg cgcgccggca gccgctcacc gtgcatggct
    13501 ggatctacgg catccacgac ggcctgctgc gcgacctcgg cttcaccgcc ggctcgcccg
    13561 cccaggccgc aagcggctac cgccgcgcga tcacggtttc aggtaggcga agccctcgcg
    13621 ctgctgcagg cgggtgacct cggccacgcc cgacggcacc cagctgacct ccgcgatgaa
    13681 ctgatccttg gcgagcttgc ggttacgcag cgtgatctcg cagacgtcga agcgcacgcc
    13741 gcgcatcttc agctgctcga ccaggtcctc gtagcggttg ccgttcgcgt ccttggcgtc
    13801 cttcatcagg aaatcgacgc cgcgcgcgtg cgacacgacg acgatgcgcg ccttcgggtt
    13861 gacttcgagg tgattgccga tgttgcgcag gccgttggag gcctgcggca atccttcgct
    13921 caggtgatag acgacccggt cctgctgcgg ctcgacgctc gcgcatcccg ccgccaggac
    13981 gagtaaggcg agggtcttgc gcaccgggct agcccttgcc gccgcaggtc ggcggcatcg
    14041 cgtcgaccgg gtagcctttg cagctccaac tcgccttgcc gccctgcagc gagggcgtca
    14101 gcgtgacctc gagcgcgttc ttctcgttcc agccgcggat ggcgccgttc tgcgacacga
    14161 tccatttcat cttgccgtgc ttcggatggt cgttctcgag cgccttgatg ccgttgccgg
    14221 cggtggcgag gctgccggat ttttccgctg cgcttcccac ctgttgcttc gccgcctccg
    14281 cgccggcgac cagcgcctgc gcggcctcgc gcatctcaga accctgcatc cgcggcagga
    14341 ccaccgcgac cgccacgaaa ccgatcaggg cgagcccggc gatgaccagc aaaccagcgc
    14401 gcatttcttt tctcccccct tttttgcacc tttcactgct gaaaatcaga ataccatttt
    14461 tgacatggcg acgcagcgac tgcggatcac cgggcgcgtg cagggcgtcg gctttcgcta
    14521 cgcgctgcaa gacgaagcgc agaagctcgg tctcgccggc tgggtgcgta atcgccgcga
    14581 cggcagcgtc gaggcgctgg cgcagggctc accgccggcg ctcgcggcgc tcgccacctg
    14641 ggcgcggcgc ggaccgcccg cggcacgcgt cacggacgtg cgggtcgagg cagcgccgga
    14701 tgaacccgca accgaaggct tcgagctgcg gccgacgctc taggcgttct tccgccgcct
    14761 gatcgcgctg accgccgaga attgccacgc ccggcaagcg gcgagcatcg cgaccggggt
    14821 gcggcggctc gccggcagcg cggagtcggc ctccgccatc tcgccgaact ccgcgagcag
    14881 ctgctcgagc ttgcgcttga gcgtcgccgc cgactcggcg gacagctcgc gcggctcgaa
    14941 gcggatgagc tcgagcggac cgtcgaagcg cgcctgcagg aattccgcca tcgcgcgccg
    15001 cgcgtaggcc ttcttggccg gtccgcccgc gcgccagagg aagtcgcgcg gcaccagcag
    15061 ccgcgcgcgc tcgcgcggcc cccactcgat cagcttcagc ttctccaggc gcgcgaacgc
    15121 gatggtgagc tcggtgcgcg cgaccgcgta gccctcgagg atctcctcga agctccagcc
    15181 gttcaggagg agccagaaca ccgcgagcag ccgctcgtcg ccggcgaggc cgagctcctg
    15241 ctcgtgcgtg agctgcgccg ggccggcgcc gccgccgcgg ctcatgcgcg cgacttcgta
    15301 gagatcgagg tcgaccgcgg cgagcacttc ctcgacgcgc gaaagggtga aagtgccgcg
    15361 cgagaacatg cgcttcacgc tcgcctcgga gatccgcagg cggcgcgcca gctcggcgta
    15421 ggtgagcccg cgcgcgcgaa cgctcctctt gagcgcctcg acgaagcggc tggccgcagg
    15481 catgcgactt acggtatcag ataacgccac cttgcaagcg cctgttgcgc cgcggcatcc
    15541 gctgtcggaa caatgccgca gggaaaacac tttcgcaact tcgagtggag gaggccatga
    15601 ccgatccgcg ttctatcccg ttgtccgctc tcgaacttag cgctgcggtc cgcgaggggc
    15661 gctcctacga cgctgcacgc ctcgatcgcc tgctgcgcgt cgatgaaggc catggcctgg
    15721 tggaggtcca ggcgagcacg ccgtggaaag cgatcgccgc atcgctgcgc cccggcgatc
    15781 cgcgcgccgc gggcacgcac accacgatga gcaccgtcgg cgacagcctc gcgcacaatg
    15841 ccgccggccc cgacggccgg ccggcagtcc tgcacgtcga atcgatcgcc gtggtgaccc
    15901 ccggtggcga gctgcgccgc gcgagccgca ccgcgcatcg cgagctgttc gcgctggtcg
    15961 ccggcggcca gggcttgttc ggcgcgctct acagcgtcac gctcgacatc ggctcgctgt
    16021 cgcgcgcggt caacaatgct gtggaacggt caggaagacc cgtcgtgccg ggacgtgggt
    16081 tgcggctgtt gctgcctccc gagaagcttc aggcattcct cgacgaggcg cgcggccgct
    16141 gcgaagaatg gcgcatggcg cttgccggcg tcgaggcgcg ccacacggtg ccggagaacg
    16201 attcgttcct gcgctgggcg cgacgcgaat acgccgcggt cacgctgcat ctcgccgagc
    16261 cggcggcgct cggcgcggcg gttcgcgcga cccagctgcg ccgcagcctg atcgacgcgg
    16321 cgatcgtgca gggcggcagc ttccccgtgt cctgcacccc ggaggcgacg cgcgcccagg
    16381 ccgaggcgtg ctacccgcag ctgcgcgagt tcctcgcgga gaagcggcgc atcgatcccg
    16441 gcgagcgcct ggtcaatgcc tggtaccgcc actaccgcac gttattcggc agtccgtgcg
    16501 ccgtgcgttg ggcgcatggg gcggttaact aactgccgaa aacaagctcg atgttctcgg
    16561 gggtgagcca atcgccgaga tccgcgagga gcttgctgtc gggccgcacc cgccagcctt
    16621 cgccgagcac gacttcgcac gcagcgttgc cgttgccata ggcgaccacc accaggcacg
    16681 cgccgtttcc ggtggcgcga taaggcgcca ggacctgctg cagccgcttg gcatcggccc
    16741 cgccgttcat ggaaatcttc aggcgggcgg cgtagcgcgc gcgcaggccg tcgaggtcca
    16801 gcagctcctc ggcgagcacg cgcaggccgc cggagaactg gtcgtcctgc accctgccgc
    16861 agaccacgag cagcgcgtct tccttcagct tctcgcggtg tctttcgaac agctcgttga
    16921 acaccgagat ctccacctgc gcggtggcgt cgtcgagcat caccaccatc atgcggccgc
    16981 ggcgcgtcat ctgcgcgcgc gccgcgacca cgatcccggc catccacacc cgctcgccgg
    17041 ggctcagctt tgcgagcgcg gtgcgcggaa agcggctcag ctcgcgctcg tagatcgaga
    17101 acagatgcgc cgaaaggtag aagccgagcg ccgccttttc ctccagcagc ttctgccgcg
    17161 tatcccacgg cggcgcctcg acggtgacgt gcgccccgcc gcgcggcgcc tcggcttcgc
    17221 cgaacaggct ctcctggctc gcctggcgct cggcctgctc ggcggcctcg aaggcgcggc
    17281 ccgcggaggc gagcaggcgc gcgcggttcg cgtcgatcgc gtcgaacgcg ccggcgcgga
    17341 ccagcgcctc gagcactcgc cggttcacca tccgcttgtc caccctgctg cagaagtcga
    17401 acagactcgt gaacggcgcc tccttgcggg cggacaagat gctctcgatc gcggattggc
    17461 cggtaccgcg gacggcgccc agcccgtagc ggatcgtgcc gcggtccacc ggcacgaagc
    17521 ggtactccga cgagttgacg tcgggcggca ggatggtgag gccgtttgcc agcgcgtcct
    17581 cgtggaactg gcgcaccttg tcggtgtcgt ccatgaccgc ggaaaggttg gcggcggcga
    17641 acgcggccgc gtggtgcgtc ttcatgtacg cggtctggta ggcgagcagc gcataggccg
    17701 cggcgtgcga cttgttgaag ccgtagccgg cgaacttttc catcaagtcg aagagctgcg
    17761 tcgccttgcc gcgggcgagg cctttcttct gcgcgccttc gacgaagctg tcgcgctgca
    17821 gcgccatctc ctcgggcttc ttcttgccca tggcgcggcg caggaggtcg gcgccgccga
    17881 gcgtgtagcc gcccatcacc tgcgcgatct gcatcacctg ctcctggtac accatgatgc
    17941 cgtaggtcgg accgaggatc ggctcgaggc gcgggtcgag gtagtcgacg cgcttgccgt
    18001 gcttgcgctc gatgtactcg gggatcaggt ccatcggtcc cggccggtac agcgcgacca
    18061 gcgcgatcac gtcttcgaag cggtcggggc gcgcgcgctt gatgagatcg cgcatgccgc
    18121 gcgattccag ctggaacacc gccgtggtgt tgcccgagga gagaagtttg taggtctgcg
    18181 cgtcatccaa ggggacctga gccagggaaa aatccgcctg tcccaagccg cgcacaaacc
    18241 gctccgccca gtcgaggatg gtgagcgtgg tgaggccgag gaagtcgaac ttcaccaggc
    18301 cgatcgcctc gacgtcgtcc ttgtcgagct gcgagatcac ctgcgcgccg tccgcggcgt
    18361 agagcgggca gaagtcggta agccgccccg gcgcgatcag cacgccgccg gcgtgcatgc
    18421 cgacgttgcg caccaggcct tcgagcttct cggagagctc gagcaactcg cgcacctcgt
    18481 cctcgttctt ctcgcgctcg gcgagcagcg gctccgcctt gcgcgcgtcg tccagcgtga
    18541 tcagcttgcc cggctgcacg ggaaccaatt tcgcgagctg gtcgcagaag ctgtacggca
    18601 ggtcgagcac gcgtccgcag tcgcgcagca cggcgcgcgc tgccatggtg ccgaacgtgg
    18661 cgatctggga aacgctgccc tcgccgtatt tttgccgcac gtaatcgatc acgcggtcgc
    18721 ggccgtcctg gcagaagtcg atgtcgaagt cgggcatcga cacgcgctcc gggttgagga
    18781 agcgctcgaa cagcaggtcg tatttcagcg ggtcgaggtc ggtgatgccc agcgaatagg
    18841 cgaccagcga tcccgcgccc gagccgcgcc ccggcccgac gggaacgccg ttctgcttgg
    18901 cccagttgat gaagtcggcc acgatcagga agtagccggc aaagcccatc tggatgatgg
    18961 ttttcagctc gaactcgagc cgctccacgt tctgcaagcc gcgcttcttc agcccgtcgt
    19021 gcgcctgctg ccggaggtaa tcctcgacgc ccactcccgc cggcgtcggg aaggccggca
    19081 ggcggctctt gccgagctcg atcgcgaggt tgcagcgccg cgcgatctcg atcgagttct
    19141 cgagcgcctg cgggatgtcg gcgaagaggc gcgccatctc gtcctgccgc ttgaagtact
    19201 gctcgggcgt gaagcgccgc gggcggcgct ggtcgccgag gatgtggccg gtagcgatgc
    19261 acacgcgcgc ttcgtgggcg cggaaatcgc cggcgtcgag aaactgcacc gggtgggtgg
    19321 cgaccaccgg caggcccagc cgcgaagcga gcgccaccga gcgcgccagc agcgcctcgg
    19381 cgtgcggctg gcccgcgcgc tgcagctcca ggtaatagcg gcccgggaag agcgccgccc
    19441 agccctgcgc gagccgctcg gcggcctcgt cgtgcccggc aaggagcgcc tgtccgacat
    19501 cgcccgacgc cgctccggac agggcgatga ggccctcggc atcctgcaac cactctcggg
    19561 cgagctcggc ccggccgcgg tgctggttgc gcagccaggc gcgcgacagc aggtcgcaca
    19621 ggcgcaggta gccggcgcgc gaggcgcaca gcaggagcag gcgaaacggc ttgtcgcggt
    19681 cggcgtcgtt ctgcagccag caatcgacgc cgatgaccgg cttcacccct gccgcccggg
    19741 ccgcgccgta gaacttcacc atgccgaaca ggttcccggc gtcggtcagc gcgagggccg
    19801 gcatggcgtc ggcccgggcg cgctcgacgg cctcctcgat gcgcacaatc ccgtcgctca
    19861 ccgagtactc gctgtgcagc ctcaggtgaa cgaaacgcgg cgctgccatg tcaaaaaaat
    19921 tgtaccggaa aggtccccat gaaacgctca ggcatagttc tttcgatcct cgccgcaatc
    19981 gcgctttctg cgtccgccct ggcgcaagat cgcaccgcgg agcttgacaa ggccgtcgag
    20041 gaggcgcgca ccgcctacct cgagctgcag gaggccgaga agcggcgcga ccagggcatc
    20101 gagtcgcagg ctggcgagcg ccaggggagc gccggcggcg gcagccggcc gaacgacgcc
    20161 tacttcgggc gccaggcgca gctcgagcag gaagtcgagc tcgcgcgcaa gcgctacgag
    20221 gcggcgcaaa agcgttggaa tgatctgaag taaagccctt accatgcgcg gggcatgaat
    20281 cccgcgccga attccgccct cctcgcgctc cttctcggcg ccacctgcat cgcgctgtcg
    20341 ccgatcttcg tacgcgtgtc cgaggcgggt ccgacggcga ccgctttctg gcgggtcgct
    20401 ctcgccgttc ccgtgctttg gatcctctat gccctcaagt ccaaggccag aacccggcgc
    20461 tattccggca aatggccgct gctgctcgcg gcgggatttg ctttcgccgg cgacctcgcc
    20521 ttctggcaca cctcgatcaa gctcacgtcg gtggcgaact cgaccctgct cgccaacctc
    20581 gcttcgattt tcgtcacgct cgccgcgtgg accttcctgc gccagcggcc gagcggcctg
    20641 tttctcgcgg ggctggccgc cgcgctcgcc ggcgtcgcgc tcctcgtgca caccagcctc
    20701 gagttctccg ctaccggcct cgccggcgat gcgctcggcg tggtgaccgc gatgttctac
    20761 gcgggctaca tcctcgccgt gaaagggttg cgcgatcgcg gcgaaaccac gctgcacctg
    20821 atggcggtga cgacgacgat caccgcgcta tttctcttcc cggtggcgct cgcctcgggc
    20881 gagccgatgc tgccggcctc ggccttcgga tggtggatgc tcgtcggcct cgcgctcatc
    20941 tcgcacgccg ccggccaggg cctcatcgcc tacgccctcg cccacctgcc cgcggcgttc
    21001 tcgtcggtga gcctgctgtt ccagccggtg atggccgcgc tgttcgcctg gctcctgctt
    21061 gcagagggcc tcgtacccct gcaggtactc ggcggaatca tcgtgctcgg gggaatctac
    21121 cttgcgcggc gcggttcgag cgcgggacaa tgattcaacc ctgaggaggg tgcatggcca
    21181 aggcaaagaa gaaggcgaag tcgaagctgc ggcgcaagaa gccgttgaag aaagtggtca
    21241 agaaggtcct caaggcggta aagaaggtcc tcaagaaacc gaggccgaag gcagccaagc
    21301 cagcgatttc tgcgcccgcg ccgctacccg gcgagcgaat cggcatagtg acgcattact
    21361 tcggccatct gtccgtcgcg gtggtcaagc tcgagcccgg cgtcaccctg cgcgtcggcg
    21421 acaacatcca cgtcaagggc cacaccagcg acttcagcca gcgcgtggaa tcgctgcaga
    21481 tcggtcacgc ctcggtcgag caggtgggga cggacgatga cttcggcctg aaggtggccg
    21541 agcacgcacg cgagcacgac gtcgtgtacc gggtgaaacc gagctagacg atcaccgcgt
    21601 cgggctcgcc cgaggacttc tcgatcgcgc cgatctcgta gaccgtttcg cctgaggccc
    21661 gcaagctctc catggcgcgc tgcacgtcgg cgcgctcgac ggcgagcacc atgccgatgc
    21721 cgcaattgaa cacgccggcc atctcgtcct cggcgacctt gccctcgcgc tgcagccact
    21781 ggaacacctc gggccgcggc caggctgcct tgtggattgt cgcaagcaaa tccttgggga
    21841 gcatccgcgg gacgttgccg gtcaggccgc cgccggtgat gtgcgcgagc cccttcaccg
    21901 cgacgccctc gagcagcttc agcaccggct tgacgtagat gcgcgtcggc gtaagcaggt
    21961 cgaacggcgg cttggcgcgc tcgaggatct tgcggatcag cgaatagccg ttggaatgcg
    22021 ggccgctcga cgcgaggccg agcagcgcgt cgcccgcgcg gatcgagcgc ccgtcgatga
    22081 tccgctcctt ctcgaccacg ccgacgcaga agccggcgag gtcgtactcg cccgcggcgt
    22141 agaagcccgg catctcggcg gtctcgccgc cgatcagcgc gcaaccggcc tgctcgcagc
    22201 ccttggcgat gccctggatc acgcttgcgg cgacctgcac gtcgagcttg ccggaggcga
    22261 agtagtcgag gaagaagagc ggctcggcgc cttgcaccag cacgtcgttg acgctcatcg
    22321 cgaccaggtc gatcccgacg gtgtcgtgcc ggtcgagctg aaacgcgagc ttgagcttcg
    22381 tgccgacgcc gtcggtgccg gagaccagca ccgggtggcg gtacttcttc gggatctcgc
    22441 acagcgcgcc gaagccgccg atggcgccca gcacctccgg gcgcatcgtg cgcctggcga
    22501 acggcttgat cgcctcgacg agcgcgtcgc ccgcgtcgat gtcgacgccg gcgtcgcggt
    22561 aggagagccc tttcgacatg ctagattgga attttacgat gcaactcatc ctcgacctcg
    22621 cggccgcggt cgagccggat ttcgataact acgtcgccgg cccgaacgcc gaagccgtcg
    22681 ccagcctgcg cagcttcgcc gccggggcaa cgcgcgacgc cgtgatgtat ctctggggag
    22741 agccgggaag cgggcgcacc catctgctgc gatcggctgc gaaggcgaat cccggcctgg
    22801 tgaccgccga cgatgtggac gatctcgacg agcccgcgca gcaaggcctg ttcgcggcga
    22861 tcaacgcggc gcgcgaaggc gcgccgccgg tgctcgccgc gggcagccgg ccgccggcgc
    22921 agctcgcgct gcgcgaggac ctgcgcaccc gcctggcctg gggcctcgtg taccagctaa
    22981 agccgttgag cgacgccgag aaggcgctgc acctgcgcgc cgaggcggcg cgccgggggt
    23041 tgcggcttcc cgacgaggtt gtcgggtacc tcctgacccg gctgccgcgc gacctgccga
    23101 gcctcaattc ggtcctcgat gccctcgacc gttactcgct cgccgccaag cgcccggtga
    23161 cccttcccct ggttcgggaa gcgctggaac gaaaatttcg gtaaatccgt ggctccgccg
    23221 ccacagtcgg ggaatcctca cctcgacagt gcccagtccg gcgagtagat tggaatcgct
    23281 ccgatgaaag ccagagccgc cctcttcctg ctgttcttgt ttgccggcca aacgctgggc
    23341 cagtccggcc tgcggctgcc gcagcaaggc gagcccggcc tcgacccgac gttcgcccgc
    23401 ggctggctcg cgccggacta cgaccgcttc ggcttcgcgc tctacacctg gcgcgaggcg
    23461 gtcggcttcg cgccgggcca gcgcatgcag ttgacctacc cgttcggtga ccgcggcagc
    23521 ctcggcatgt cgatggccag cggcacctac tacgtggacg agaaccggca ggtttcgctg
    23581 tacggcagct attcgttgtc cgcggactgg gcgctcagcg ccgagacgct ggggcgcgac
    23641 gcctcgggtc tgttccgcct tcaggacttc cgcatcggcg tccagcgccg gttctaagaa
    23701 gaagttcgcc ttaaactccg ggcgctcatg cgcctggccc tgttcgacct cgacaacacc
    23761 ctcctcgccg gcgacagcga ctacgagtgg ggccagttca tgtgcgacca gggcgtggtg
    23821 gatcgcgcgg cctacgaagc gcagaaccgc aagtactacg agcagtacaa ggcgggcacg
    23881 ctcgacatcc acgagttcct cggcttcgcg ctgcgcccgc ttgccaacca taccgccaca
    23941 gagctcgagc gctggcacgc ccaattcatg cgcgcgcgca tcctgcccat gatcaccccg
    24001 gcggcacgca cgctggtccg ccgccacctg caggccgggg agctctgcgc cgtcatcacc
    24061 gcgaccaaca gcttcgtgac tgcgccgatc gcgcgcgagt tcggaatcga gcacctcatc
    24121 gccaccgagc ccgagcggcg caacggccgc tttaccggcc gcgtggccgg gacgccgtgc
    24181 tttcgggacg gcaagctcgt gcgcctggac gaatggctcg ccgggctcgg ccggcgcctg
    24241 gccgatttcg aggcgagcac gtgctacagc gactcgcaca acgacctcgc gctgctcgag
    24301 cgcatgacgc gcccggtggc ggtcgacccg gacgagactc tcgcgcgcga ggccgcccgc
    24361 cgcggatggg aacgcatctc gctccggtag aatccccggc ctcgaatgat caagcgcttc
    24421 atccggcggg tgctggggct cgcctccgaa ggcatgcagc gggtgccgcg cgagaggcac
    24481 ggcctcgcgc gcgagcgcct gagccccgcc gccgccaagg tgtgcgccgt gctgcgcgag
    24541 gccggctttt ccgcctacgt cgtgggcggc gcggtgcgcg acctgctcct cggcatcgtc
    24601 ccggtggatt tcgacatcgc gaccgatgcg cgcccggagc aggtcaagcc gctctttcgc
    24661 cgcgccctga tcatcggccg gcgctttcgc ctggtgcacg tcatgatggg ccccgagacc
    24721 gtggaggtgt ccaccttccg ctctgccgac gacacctcgg agaaggacga gcacgggcgc
    24781 gtgctgcgcg acaacgtgtt cggcacgcag gaaggcgacg cgctgcgccg cgacttcagc
    24841 gtgaacgccc tgtactacga cccggccacc gaggaagtcg tcgacttcca cggcgggctc
    24901 gccgacctga agaagcgcgt gatgcgcgtc atcggggacg cggaaacccg ctaccgggag
    24961 gatccggtgc gcatgctgcg cggcgtgcgg ctcgcggcga agctcggcct cacgctcgac
    25021 ccccacacgc gcgagccgat ccgcagcctc gcgccgctga tggagcgcgt tccgccggcg
    25081 cgcctgttcg acgaaatgct caagctgctg ctctcgggcc acgccagcgc gtgcctgcgc
    25141 cagctgcgcg aggtcggcct gcacaagggg ctgctgccgc tgctcgacgt gatcctcgag
    25201 cagccgctcg gcgagcgctt cgtcacgctc gcgctcgccc agaccgacga gcgggtgctc
    25261 gccgatcgtc cggtgtcgcc ggcgttcctg ttcgcggcgc tcctgtggca cgaggtgctc
    25321 gcggcgtgga agtcgcgcca ggcacgcggc gagcgcgcga taccggcgct cgagaccgcg
    25381 atggacgagg tgctcgacac gcagtgcgcc aagctcgcca tcacgcgccg gctgaccgcg
    25441 acgatgcgcg aagtctgggc gatgcagccg cgcttcgacg gccgctccgg caaccgcgcc
    25501 ttccgcacgc tcgaggcgcc gcgcttccgc atggcctacg acttcctcgc cctgcgcgcg
    25561 gcgagcggcg aagtgccggc cgagctcgag gcatggtggc ggacgttcca gagcgccgac
    25621 gcggacacgc gcaaggcgat gctgctgccg gacaccgccc cgagaaaacg ccgccggcgc
    25681 cgccgcaaga aaggcccggc ggctgcggcg tccgcccctg aaacggcgtg accatcgctt
    25741 acgttggaat cggctccaac ctcgaggagc cggaaacgca agtccggaaa gcgttcgatg
    25801 agctggaccg catgccccat acccggctgg tgaaaaaatc gtcgctctat cgcagcgccc
    25861 cggtcggtca tgccgcgcag ccggatttcg taaacgccgt cgcccggctc gagaccgggc
    25921 tgccggccga gcgcctgctc gccgagctgc aggagatcga ggatcgccac ggccgccggc
    25981 gcagcttcgc caacgcgccg cgcacccttg acctggacgt cctgctttac ggcgagcgct
    26041 cgctcgacct tccaggcttg aagatcccgc atccgcggat gcatgagcgc gccttcgtgc
    26101 tgcggccgtt gctcgagatt tctccggatc tgcaaccgcg tttcaacgcc tgcctcgacg
    26161 cctgcgcaga ccagaagatc gaaaggatcg attgaatgca actctccccg agcgtcacct
    26221 gcggcctgct cctcgtcgcg tcgaacgtct tcatgacctt cgcctggtac gcgcacctca
    26281 agaacctcgc cgccaagccc tggtggatcg cggcgctcgc gagctggggg atcgcgtttt
    26341 tcgagtacct gctgatggtg ccggcgaacc gcatcggcta caccgagctc tcgctgccgc
    26401 agctcaagat cctgcaggaa gcgatcacgc tcggggtgtt cgtgcccttc gcggtcctgt
    26461 acatgaacca gccggtgaag ctggactacc tgtgggcgtc cctctgcatc atgggagcgg
    26521 tctatttcat tttccgtgga taagttccgc cacctcgtgg tcgaggggcc gatcggcgcc
    26581 ggcaagacga gcctcgcgcg gcgcctcgcc tcgcgcctgg ggacggacct cgtgctcgag
    26641 cgcccggagg agaacccgtt cctcgcgcgc ttctacgacg acatgccgcg ctatgcgctg
    26701 ccgacgcagc tctttttcct cttccagcgc gcgcgcatgc tcgagccgct cgcgcagccc
    26761 gacatgttcg ggcgcgccgt catcgccgac ttcctgctcg acaaggatcc gctctttgcc
    26821 cgcctcacgc tgtccgcgga cgagctcgcc ctctacgaga agatctacgc ggtgctgcgc
    26881 ctgcgctctc cgccgcccga cgtggtgatc tacctgcagg cgcagcccgc ggtcctgatc
    26941 gagcgcgtgc ggcgccgagc ggcgcgctac gagcgcgaca tcagcgagga ttacctcgcc
    27001 cgcctggtcg agggctacgc gcgcttcttc taccactacg acgcctcgcc gctgctcatc
    27061 gtgaactccg agaacctgaa cttcgtcgag cgagatccgg acttcgagct cctgatctcg
    27121 cggctgcgcg cgatgcgcag ccggcgtgag ttcttcaacc tcggctgatg cagatcctgc
    27181 gcgacatcgc gagcctgcgg cgcgccgtcg ccgccgggac cgtcgccttc gtcccgacca
    27241 tgggcaacct gcacgccggg cacttgtcgc tggttcggca agggcgccgc cacgccccgg
    27301 cagtggcggt gagcatcttc gtgaaccgcc tgcaattcca gccgggcgag gacttcgagc
    27361 gctacccaag gaccttcgag cgcgactgcg agctgctcga gtccgagggg gtggaatttc
    27421 tgttcgcgcc ggatgaaaag gtcctgtatc cggaaaaaca acaagtcagg gtgcaggcag
    27481 gccctctcgg cggggagctg gagggccgct tccggcccgg gttcttcgac ggcgtggcga
    27541 cggtggtgct gaagctcttc aattgcgtgc agccgcgcgt cgcagtgttc ggcaagaagg
    27601 actaccagca gctcatggtg gtgcgggccc tggtgcgcca gctcgacctg ccggtcgcga
    27661 tcgaggcggc cgagaccgtg cgcgagcccg atggcctggc gatgagctcg cggaacagct
    27721 acctgtcccc ggccgagcgg gctgaggcgc ccaggctgta ccgggtcgtg cgggaagtgg
    27781 cttccggaag gtcgggcccg ggcgctgccg tccagttact ctcaaaagca ggctggaagc
    27841 cggactacgt ggaggtgcgc cgccgggggg acctggcagt cccgcggccc ggggagactg
    27901 ggggggtcgt gctggctgcc gcccggctcg gacagacccg gctgatcgac aacatcgagt
    27961 tctaaccctc cagttctagg ggggacccct aggacgagag gctcaattag gacggatagg
    28021 gttcgaaggt catggcttcg accgggcagc ccatggtgct ggcgagcttg tccatggcct
    28081 cgtcggtgag ctgcacgggc tggaactccg cttcatgccc cttggaggag atgaaggcct
    28141 tggccgcggc ctcggtgttc caggtcacca ccgggtccgg ggtgaccccg gggacctgcc
    28201 ggcagatcgg aaaaccgtcg gatttgtaga cgatggcgta catcgaccgg ctgcgccttc
    28261 ctcccgggct tgaatttacc cgagttcgga gcaccgcaac aaggcggttt tataagggaa
    28321 accctgattg caaagccttg taagggcggc gtctaatcat cgcgggatcc tggcctgtcg
    28381 tcccctggcg acggacccgg tacccggaag cccccgaccc ggggggcact acttcttggg
    28441 gaaggagaag acaaatgagc aacatgaacg atgcagtggg cctgtgcatg ccgtacagca
    28501 agacagacag ccggtacgag accgcattcg agccggccat caacaccgtg tgggcttatc
    28561 tcaatccgaa gggcgtgccg tgctacagcc tggagctgct caaggcgatg atcgcgcacg
    28621 acaagcaact cgccgacaac ggcgggcgcg tggacttcga aggcgagatg cgcaaggtgg
    28681 attactacgt gacgagctcg cgcacgccgg gcgtgttcaa cctcggcggc gacctggcgc
    28741 tgttcgtgct gctgatcaag accggcgacc gcgaggcgct ggcgcagtac gcgaagctct
    28801 gcatcgacgt ccagtacccg cgcacccaga gcttcttcag cccgacgctg accagcatcg
    28861 cgctggtgca gggcgacgcg ctcggcggcg gcttcgagtg cgcgctcgcc tcggacgtga
    28921 tcatcgccga ggaaagcgcc cagctcggat tcccggaaat cctgttcaac ctgttcccgg
    28981 gcatgggcgc gtgcagcctg ctcgagcggc gcatcggcat gcgcgccgcg gaggagctga
    29041 ttctgtccgg caagatgctg ccggcgtaca agctgcacga gatgggcgtg atcgacgtgc
    29101 tggcgccgaa cggccagggc gagagcgcgg tgcgcagcta catcgccgac aacgccaagc
    29161 gccgtaacgg catgcaggcc atgttccgtg cccgccagtt cgtgcggccg gtgacgcgcg
    29221 aagagctgga cggcatcgtc gagctctggg tcgacgcagc gctgcggctg ggcgagcgcg
    29281 agatgaagat gatgagccgg ctcgcacgcg cgcaggcccg ccgcatggag cacgagcacg
    29341 caccgcggcc ccaggatgcg gccgcgatgc tcgagccgct ggcgatggcg agctaagcgc
    29401 cgccggtccg accgggatcg agttgcgcga gcaggcgcgg ccacagggtc gtgccgaccg
    29461 cgcggcggaa gaacccgaag tgcccgatgc gccgcacttg cgcgtcgggc ggagtgaaga
    29521 gaagcagctc aggagaagag aggcgaggaa agtaggaaag cagccgcttg gtgccgtcta
    29581 cggtagcaaa ggcgtcatcg ctgatggaaa tcgcaatggc cggacactgc aaggcggcgc
    29641 agtggcccag gagccggagc ctgcgctccc ccgcaggctc cgctccgttc ggccgcaggt
    29701 ccggccggcg gcgctccgcc cactcgagtg ccatgccggc cgggaggtct tccccaagcc
    29761 cgagccggtc ggccggaaag tagcccacga atcgcgtcag cgccggcatc agcccgtgcc
    29821 aggcgaaagc catcggcagg cggtacaggg gccggtagtc cccgtagtag ccggtatggg
    29881 cgccgaccag gacgatgcgg ctctgctcgg cggtgttggg tgcgccgccg agcggcaggc
    29941 agccgatcga atgggataac cccaggatct cgtcgcgcgg gaagcgctcg cgcacccagg
    30001 cgatcgcggc cgcggcgtcg tactcggccc aatcctcgat cgtcgcctgg aacccacgca
    30061 gcgcgcgcgg gcgcgacagg ccgatgccgc ggtagtcata tgtgagcacc gggattcccc
    30121 actccgcgag aaaggtcgcg aaccggcggt agcgcaaagc cgggatgccc gcgcccccgt
    30181 gcagcacggc gacccggcgc ggagcgcgcg gcctgagcgc ggcatagaag acggcgccga
    30241 tctcgtagcc gtcggccgca cgcagccgca gctgccggcc ttcgcctgcg tccggaagtt
    30301 ccgctctacg ctgaaagtac gcgcgtccca cgacaatcga tcataaccgc tgccgctaga
    30361 ccgctgaaga tttcctttct tcgacgtaac gctcgagctg cgctcgcacc gtggcgagct
    30421 cttccgtgag cgtgcgcagc agtcccggcg cctgcagcct tagctcggcg tccgacagct
    30481 tgtcgtagct cgagcaaagg cgcgtcagcc ggtcggtccc catgctggcc gaagagcctt
    30541 tcatcgcgtg cagcagcgag cgcagctcgt ggtagttgcg cgcggcgagc gcctgctcga
    30601 cgcggccgag gagcgtggag ttgtcggcga tgaacacgcg gatcaacttc tcgacgaagg
    30661 ccaccgacga gccgagctcg cgcaggtggt cgatggtctc cagattgacg atcggcaccg
    30721 gctcgaagcc tcttgccccg tgcttctggg gaatcgcctc ggcgggctgc gcccgctccg
    30781 ccgccgcggc gcacagcgtc tggatctcct ccagcagctt cagcgcctcc accggcttcg
    30841 gcaggaaggc gtcggcgccg gcctcgagcg cctcgcgctt ggtccccggg gtcgcgtcgg
    30901 ccgacaggat gatcaccggc aggcgctcgc ggccgcgcgt catcaggcgc agcgcctgca
    30961 gcgcctcgat gccgcccacc cccggcatgt tgcggtcgag gatcacggtg tcgaacgcgt
    31021 cgcgctgcag cgcgtcgagc gcctgctcgc cgtcgttggc gagcgtgacg ctatgcccgc
    31081 cgcgctcgag gatcttcccg atgacctcgc ggttggtcgg attgtcgtcg gcgaccagca
    31141 cgcgcagctt cttcgcgctg ccgcggcgcg catagtcctg cagccgcacc acgccttcgc
    31201 gcatctcctc gcccgccgac accgagtgca gcacgttgaa gagctggcgc ttgtcgaacg
    31261 gcagctcgag gatggcggtg aagcccgagc tgagcacgtc gaagcgctgc acgtcggcgt
    31321 cgcgcggcac cgcgaggatc gtgtgcggcg tcgggttcgg cgcggcgcgg cggaagcgct
    31381 gcgcgagctt gggatcctcg ccgctcgcgt agagcagcgc gctgtggtag ggcttggcga
    31441 gcgagatttc cgccaccagg cgcgccacgc cctcctcgac cgtcgcctcg gcgaccgggg
    31501 ttacgcccca gcccgcgagc gcctgctcga ggggctcgcg ctgcgcctgc gggaagccga
    31561 ccagcagcac tctcgcgccg gcgaggtcgc ccgctccggc cagcccggcg cgctcgggct
    31621 gcttctcgag cgcgatctcg aaccagaagg tgctgcccag cccgaccgcg ctctcgagtc
    31681 ccagcttgcc gcccatgagc tccaccagct gcttcgcgat ggtggtgccc aggcccgtgc
    31741 cgccgaagcg ccgcgaggtc gactggtcgg cctgcgtgaa gctctcgaag atgcgcgcct
    31801 gcgcttcggc cgggatgccg atgccggtgt cgcggatcga gaacttgagc cgcacgctct
    31861 tgtcgctctc ggaatgcgcc gagacgtgca ccgtaacgct gccgcgctcg gtgaacttca
    31921 ccgcgttgcc ggcaaggttg atcacgatct ggcgcaggtg gtgcgggtcg ccgcgcaccg
    31981 ccggcggcac ctcgggcatg atcgagacca cgaactcgac gcctttggag gcggcctgcg
    32041 cggcgacgat gcggcaggtg ctgttgacca gcgcgtgcag gtcgaagtcg gtcttctcga
    32101 gcgcgagctt gccggcctcg atcttggaga agtcgagcac gtcctcgacg agtcccagca
    32161 gcacgcgcga ggagccgcgc agggtctgca gcatgtcggc ctgctctcgc gtgagggtgg
    32221 tgtcgcgcag caggtcggcc atgccgatga tggcgttgag gggcgtgcgc atctcgtggc
    32281 tcatcaccga gatgaagcgg cgcttcgcca cgttcgctgc ctcggcacgc gccagcgcct
    32341 cgaacatgcg ccgcaccagc gacagcacgt acatgctgac aaagaacatg cccagcagca
    32401 gccccacgcc gagcgcggtg tgctcgcgcc accatgggtt caggtagatg gcgatcgcga
    32461 agccgaccac gctcatgacg agctcggtca gcaggtactt cggaccgaag cggaagccgc
    32521 tgccgtacgt gacccagagg tagatcagga agaggggcgc cgcccactcg ccggagatcg
    32581 ccatgtagca gctgatcgtc gcgacgtcgg cgagctgggc gaagatgcgc cgcgaaggcg
    32641 agatgtgcgt ggtgtaggcg atgcgcagga agatcagcac gccgaccacg aactggatgc
    32701 tgccgaccca gaggatcagc tcgcgctcgg tcccggtggt gccgggcagc aggtacagca
    32761 ccagcagcgc gccgatcacc aggcgcagga cgccctgctc gtgctcggtg tccgggcgcg
    32821 ccttgaggcg cgccttcagg cctgccagca ggccgttggg cgccgcggcg gcgggggcgc
    32881 taggctcggc catcgcgcca ttcgtgctgg atctcgagca cctgctctcg cgcgccgagg
    32941 aacgcctcga ccatgcgcgg gtcaaagtgc cgccccgcct gcgtgcgcac gtactcgaat
    33001 gcctgctcgc tcggccaggc tttcttgtag ggccgcaccg aggtcagcgc gtcgtacacg
    33061 tcagccaccg cgacgatgcg cgcgcacagc ggcacgtggt cgccgaccag gccgttcgga
    33121 tatcccgagc cgtcgtactt ctcgtggtgc ccgagcgcga tcagcgcgcc catgcgcacg
    33181 tacttggacg cgctgccctt caggatctcg tggccgatca ccgggtggcg gcgcatcacc
    33241 tgccattcgt ctttctctag cgcgttcgcc ttgagcagga tctggtccgg gatgccgatc
    33301 ttgccgatgt cgtgcagcgg cgcggcgagc tcgatggtct cggcctcgtc gcgctcaagc
    33361 ccgagcgcgt gcgcgatcag gcgcgaatag cgcgacatgc ggatcaggtg gttgccggtc
    33421 tcctcgtcgc ggaattcgcc ggcgcgggcg agccgcagca gggtctcctt ctcgcgctcc
    33481 ttcacttcgc gcgtcgcgtc ctcgaccatg tgctcgagga ggcgccggcg gtcctccagc
    33541 gcgagctgct ggcggcgcag cgtcaggagg ttgcggcagc gcgcgaggca ctcgcgcgca
    33601 tcgaccggct tggtcaggaa gtcggtgatg cccgcatcga gcgccgcgta gcgcaccttg
    33661 cggtcgtcgt gcaccgtgac catcacgatc ggcacgtgct cgtagccggg catcgccctc
    33721 aggcgcttca cgagctcgat gccgtccatc tcgggcatca tgtagtcgac cagcaccagg
    33781 tcggagacgt tgcgcgtcgc ccagaccacc gcgtccaccg ggcgcgcgaa cccttccacc
    33841 acgacgcgct cgtccaggct ccggaccacc tgctcgagaa tggcgcggcc ggtcgactga
    33901 tcgtcgacca ccatcaccgt gttgcgcgcc gaaggcagca cccgcacttg cgccttcgcc
    33961 atatcccccc caaagatctg atttctactg ggaaaagcct acccccgccc ccctgccctg
    34021 tccagcaaag gcgcccggtt cacgcttact ttttacaaac tgtggtgccg gaagagagac
    34081 tcgaactctc atggtcttgc gaccggcgga ttttgagtcc gctgcgtcta ccggttccgc
    34141 cattccggct gggaaaaggc ccgaaattat aattccgggg gtgcgggtct ccgagttcga
    34201 ctacgagttg ccgaccgaac tcatcgccca gcacccgatg ccgcagcgca gcgcgagccg
    34261 cctgctgcgc ctggatgcga gaaccggcag ccttcaggac ctcaaattca ccgacctgcc
    34321 gcaactcgtc gacgagaaag atgcgctcgt cctgaacgac acgcgggtgg tgaaggcgcg
    34381 cctcgccggc cgcaaagcga gcggcggcaa gatcgagctc ttcgtcgagc gcgcgctcgg
    34441 cgagcgcgag gcgctggcgc tcatcaaggc aagccatccg ccggcccccg gctcgcaggt
    34501 tttcatcgga gaccttccgg tgacgatcga gtcgcgcgac ggcgagctgt atcgagttcg
    34561 tttttctcaa ccgatagagt ccgtgctgga gcgtttcggg gcagtgccgc taccgccata
    34621 catccggcac gcaccgctcg ccgaagatgc cgagcgctac cagacggttt acgccgcggt
    34681 tcccggcgca gtcgccgcgc cgaccgcggg gctgcatttc gatgaaggga tcttgaacgc
    34741 cgtgggtgcg cgcggcgcca ccgttgcgaa aatcaccctg cacgtcggct tcggaacctt
    34801 ccagccggtg cgtgttgacg aactggaagc ccaccggatg caccgcgagc gctaccgcat
    34861 ttcttcttca accctgcaac tgatcaagga ccgccgcgtg ctcgcggtcg gcacgacggc
    34921 gttgcgcgcg ctggaagccg cacacctgag cggcaagctg gagggcgaca ccgacctctt
    34981 catcaccccg ggattccggt ttcgcgtggt gcagcgcctg ctcaccaact tccatctgcc
    35041 gaaatcgacg ctgctgatgc tggtctcggc gtttgccggg ctcgagaata tccgcaaggc
    35101 ttacgcgcat gcgatcgccg agcgctatcg tttcttctcc tacggcgacg cgatgctgat
    35161 cgagcgatga acttcaaggt cactgcaagg gatggcgcag cccggcgcgg cgttctcgag
    35221 ctcgcccacg ggagcgtcga cacgccggcg ttcatgccgg tcggcaccta cggcaccgtg
    35281 aaggcgatgt cgcccgagga ggtggcgggg ctcggcgcgc agatcgtcct cggcaacacc
    35341 ttccacctgt ggctgcggcc ggggctcgag gtgatcggga agcacggcgg cctgcaccgc
    35401 ttcatggcct gggacaagcc gatcctcacc gactcgggcg gcttccaggt ctttagcctg
    35461 ggcaacctga gaaaaatcag cgaagaaggc gttcaattcg cgtcgccgat caacggcgat
    35521 cgcctgttcc tcacacccga ggagtcgatg cgcatccagc gcgtgctgaa ttccgacatc
    35581 gcgatggtgt tcgacgaatg cctcgcctac ccggcgaccg agcagcaggc ggcggaatcg
    35641 atgcgcatgt cgatgcgctg ggctgcgcgc tcgaagaagg cctgggacag cggtaacgcg
    35701 cttttcggca ttgttcaagg gggggtctac ccggcgttgc gcgaagaatc ggtccgggcc
    35761 ctgaaaacca tcgggttcga cggctacgcg atcggcgggc tggcggtcgg agagccgaag
    35821 gaggagcgct cgaagatcct ggagggaacg aagctgcccg aggaccggcc ccgctacctg
    35881 atgggcgtcg gcacccccga ggacatcatc gaggcggtgg gcgcggggct ggacatgttc
    35941 gactgcgtgc tgccgacgcg caacgcgagg aacgggtggc tgttcacgcg cttcggcgac
    36001 atccgcatcc gcaacgcgcg ctaccgcgac gacaccgcgc cgctcgacga gacctgcggc
    36061 tgctacacct gccgccactt cacccgggcc tacctctacc acctggacaa ggcgaaggag
    36121 atcctcgggg cgcggctcaa tacgctgcac aacctgcact actaccagga gctcatgcgg
    36181 gagctgcgcg gcgccatcga gcaaggaatg ttcttgaaga aaacccgaca gctgctggaa
    36241 gagcgcaggc ggctgttata atttccgccg ttttttccct ctggagtccg cagtgatcat
    36301 ctcgcccgcc ttcgcccagg ccgcgcccag cggcgattcg ggatacatcg gcctgctgcc
    36361 gattgtcctg atgttcgtac tgctgtactt cctgatgatc cggccgcaga tgaagcgcgc
    36421 caaggaaacg aagtcgatga tcgaggcgct gcagaaaggc gacgaggtga tcaccgccgg
    36481 cggcgtgctc gggcgcatca cccggatcag cgacgcctac atcagcctcg agatcgcgcc
    36541 gaacctggag atcaacgtgc agaaagcggc gatccaggtc ctccttccga agggcacgat
    36601 caaaagcatt cagtgaacag atatcccacc tggggctatg cgctgatcgg actcgcgatc
    36661 gcgttggcgt tcctctacac gctgccgaat ttcttcggcg agtcgcccgc ggtgcaggtt
    36721 tcgagcgccc gggcgacggt caaggtcgac gcggcgctgc tgcagaaggt cgaggatacg
    36781 ctgaagaagg ccggcatccc ctaccgcggc gcggtgttcg acccgagcgg catccgcgtg
    36841 cgcttcgacg acaccgatac gcaatacaag gcgaaggacc tgatccacgc cgagctcaat
    36901 cccggcgccg cgccgcaggc cacgccggaa gacgacccga aggtgagcta cgtcgtcgcg
    36961 ctcaaccttc tgccggcctc gccaagctgg ctgtcggcga tccgcgcgct gccgatgtac
    37021 ctcggcctcg acctgcgcgg cggggtgcat tttctgctgc aggtggacat gcagggcgcc
    37081 accaccaagc gcctggagag catcgccggc gacatccgct cgcagctgcg cgagaagaac
    37141 gtgcgccacg gcggcgtgac gcgcgaagga cagaccctgc gcgtgcgctt ccgcgacgcc
    37201 gcgatgcgca accaggctcg cgaggtgatg ctgggcaatc tccccgatct cacgatcacc
    37261 gagcgcgagg atggccagga cctgctgctc gtcgccacgc tcaagcccga ggcggtgagg
    37321 cgaaaccagg aatacgccct caagcagaac atcaccacgc tgcacaaccg catcaacgag
    37381 ctcggggtcg ccgagccgat catccagcag cagggcgccg agcgcatcgt ggtgcagctg
    37441 cccggggtgc aggacgtagc gcgcgcgaag cgcctcatcg ggcgcacggc gaccctggag
    37501 atccgcatgg tggccgagga gcacaccaac cccgaggcgc tcgccgccgg ggcgcgcggc
    37561 cagccgccct tcggcaccga gtactacgtc gagcgcaacg ggcagccgct gctcgtgcgg
    37621 cgccaggtcg tgctcaccgg cgatcgcctc accgacgcgc agccgggctt cgacaaccag
    37681 accaacgagc ccgcggtgca cctgcgactc gacggccagg gcgcgcgcat cttccaggag
    37741 gtgacgcgcg agaacgtcaa caagcgcatg gcgatcctgc tgctcgagaa gggcaagggc
    37801 gaggtgatca ccgcgccggt gatccgcagc gagatcccgg gcgggcgggt gcagatctcc
    37861 ggccgcatga ccaccaccga ggcgaacgat gtcgcgctcc tgctgcgggc gggctcgctg
    37921 gccgcgccga tggagatcat cgaggagcgc acggtcggcc cgagcctggg acgcgacaac
    37981 atcgagaagg gcttcaacgc gacgagatgg ggcttcatcg ccatcgtgct cttcatgtcg
    38041 gtgtactacg tgctgttcgg cgtcatctcg tcgatcgcgc tcgcggtgaa cgtgctgctg
    38101 ctgatcgcgc tgctgtcgct cctgcaggcg acgctgacgc tgcccggcat cgccgccatc
    38161 gcgctcacgc tcggcatggc gatcgacgcc aacgtgctga tcaacgagcg cgtgcgggag
    38221 gagctgcgcg gcggcgcgac gccgcaggcg gcaatccacg ccggctacga gcgcgcctgg
    38281 gccacgatcc tcgactcgaa cgtcaccacg ttcatcgtcg gcgtgtcgct cctcatggtt
    38341 ccgggggcgg tgcgcggctt cgcggtcgtc cactgcctcg gcatcatgac ctcgatgttc
    38401 tccgcggtcg tggtgtcgcg cgccatcgtc aacctggtgt acgggcaccg gcggcgcgtc
    38461 gcgcacctct cgatcggcga caccgcatgg aaatgagagc gcgctagatg gagttcttca
    38521 agatccggcg aaccatcccg ttcatgcggc atgcgctcgt cttcaacatc atctcgctcg
    38581 tcacctttct cgccgccgtc ggcttcctgg cgttccgcgg cctcaacctc tcgatcgagt
    38641 tcaccggcgg cacgctgatc gaggtcggct accagcgggc ggctgacgtc gacgccatcc
    38701 gccgcacgct cgagggagcc ggctacaagg ccgaggtgca gaacttcggc tcctcgcgcg
    38761 acgtgctgat ccgcatgcac ctggagaaga accagagcag tgcggacctc tcgcagaagg
    38821 tcgtttcaat tctccaggag caggacaaga gcgcagagct gcggcgcgtg gagttcgtcg
    38881 gcccccagat cggcagcgag ctggtggact atggcgcgct cgcgctgctc gtcaccgcgg
    38941 tcggcatcgt gctgtacctc tggatgcgct tcgagtggcg cttcggcctc tccggcatca
    39001 tcgccaacct gcacgacgtg gtgatcatcc tcggcttctt cgccttcttc cagtgggagt
    39061 tctcgctacc ggtgctcgcc gcggtgctcg cggtgctcgg ctactcggtg aacgagtcgg
    39121 tggtggtgtt cgaccgcgtg cgcgagaact tccgcaagat gcgcaaggcg accacggccg
    39181 aagtcatcga ctccgcgatc accggcgtga tctcgcgcac catcatcacc cacctgtgca
    39241 cgcagatgat ggtcaccacg atgctgatct tcggcggacc ggcgctgcac tacttcgccc
    39301 tcgccctcac catcggcatc tgcttcggga tctactcctc ggtgctggtg gcgagcccgc
    39361 tggtcatgtg gctcggcgcc cggcgcgagc agttcatcgt tccggccaag aagaagcccg
    39421 gcgaccggcg cgacgagcac gaaggcgccg tcgtctgaat tcggggtcag ggtcgcaatt
    39481 cgtttttacg tacgtgaaaa cgaattgcga ccctgacccc gaattcactt gaggatctgg
    39541 atcaggttcg agtagtcgtc ggtccaggtg cgccactcgg ccttgtcctc gatcgcctcc
    39601 gggctgcgct cgacgatcgc ctcgcgctcg agcgcggcgg cgtcgcgcga taccagcacc
    39661 cagtcggtgc gcgagcggcg cgagtcgact gcctcgtcgg ggtcgtcgcg caccagcgcg
    39721 acgtgcgcgc cgtgctcctg cgcgaggcgc gccacgaccg ggatcaggtc gaggaagcgg
    39781 ttcgagacgt ggaacgcgac gatgccgtcg ggcttgacgt gcttcaggta cacgccgagc
    39841 gcctcgcggg tgatgaggtg gaccgggatc gcgtcgctcg agaacgcgtc gaccgccagc
    39901 acgtcgaatc cctgcggcgc ctcgcgctcg agggcgagcc gcgcgtcgcc gaggacgagc
    39961 tcgaccttcg ccttgctgtc gccgaggtag gtgaattcgc tctgcgccac gctgatcacg
    40021 cgggcatcga tctcgtagaa gcggtagacg tcgcccgggc ggccgtaggc cgcgatggtg
    40081 ccggtgccca ggccgatcac cccgacgcgc gccggccgct tgcccaccga gcggatcgcc
    40141 gcgccgatgc ccgagtcctc gtggtagtag gtcgtgagca tggtgcggaa cttgtcgtgc
    40201 aggtactgct cgccgtgcat gatggcgccg tgcaccagcc gccgcaggtg cgactcctcg
    40261 ccggccaggc cgtactcttt cacccgcagc acgccgtaga agctgcgcga ggcgatgagc
    40321 acatcgcgct ggtatcggaa tccgtcgtac gccgcgcagc cggcgattcc cagcagcacc
    40381 gccaggctgc catagcgcgc aaaggcgttg aggttcgcga agcgcaccgc cgcgagcagc
    40441 gcgagcgcaa ccagagcgat accgagctcg tagtacccgg tgaacgcgag cggcgccacc
    40501 accgccacca gcagcccgcc gagcgcgccg cccgccgaca ccgcgagata gaaggcggtg
    40561 agccggctcg gcgccggccg cagccggtag agctcgccgt ggcagaaaag acagccgagg
    40621 aacagtcccg gcaggaagat cccgagctgc agcggcaggt cgaaacggaa atcgtcgtcg
    40681 acgatcagcc atcccatggc gccgatccac gccaggagca gcgaccacag ccaccgcggc
    40741 cggtaccagc ccttgccctc gaaagtgatg atgaagctcg cgaggtagag cgtgagcggc
    40801 gcgagccaca gcaggggcac cgaagccacg ttctgcgtca gatggttcgt caccgcgagc
    40861 agcagcaccg agccagtagc ggccagcgcg agccacaaga catagggaga tctttcggtc
    40921 ggtgtttcgt ccgcggtcgc cgcctccatc gcctgccgcg gcgtcagcca ggccatcgcg
    40981 gcgcaaagaa ccgcgaacac gccgaagagc catgaccaga cgcccacctg ctgcgcgccg
    41041 ccaaggaagg gctcgacgac gaagggatag ccgacgagcg cgaccagcga ggcgaggttg
    41101 gacaccgcga acaggcggta cgggttctcg cccggccgcg cgcgcgcgaa ccacgcctgc
    41161 agcaacgggc tcgtcgccgc gagcaggaag tacggcaggc cgaccgacgc gccgaggagg
    41221 agcaggatgc ggctcaccgg ctcctcgtct cccgccggct tccaggcttc ccccggcgcg
    41281 atcggcaggg taaggattgc cgctaacaag agaaccgtat gaatgccggc ctcgagtctc
    41341 ttgctcgaga gcttcgccag ccaatgtgcg taggcatagc ccgcgagcag cagcgcctgg
    41401 aaaaacagca tgcaggtggt ccacaccgcg gcgctgccgc cgaaccaggg caggatgagc
    41461 cgcgcaatca gcggctgcac caggaacagc aggaacgacg acaggaagat cgtgccggcg
    41521 tagagcacta acgaatctct ttcgcgaagg gcttccccag catccaggcc gcgatcgcgt
    41581 cgacggtctc ggcttccttt ccgaagaagc cgtgcggcgc caacggctcg cagggcccgc
    41641 tttcggccgg cttgccgccg tgcaccgtga tcagcggata gcgcttctcg aggcgcgccg
    41701 cgtcgcgata cggcgtggca aagcaactgt cgtccgcgtg atgcaccagc agcgccggca
    41761 ttttgatcgc cgaccagtcg aacccggcca gcaccgggcg gggattcgtc cggggcccct
    41821 cgtagaacag cgacgacgtc agtaccgcgc cggcgacctc gccgtcgagc gccactgcga
    41881 gatacgccac ggaaaccgtt ccgcggctcg tgccgaccag gaacacgggc agcccgggat
    41941 agcgcttctt cacctccgcc accacggcgc gaatatcggc ggcatgcgga cggctcgcgc
    42001 gaaatccgtc gctcatcccg tcgccggctt tctgatcggt cgcgttgtcc aggagcagcg
    42061 gcaggatgcc gttgcggatg aattcgcggc gctcgcgcgg caggaagttg ccctggctga
    42121 agcggatctg gccgccctcg gtccgcagat tgatgcggcc gccgccgccg gcgaagagga
    42181 gcgccgctgc ctgcggcttg cgctcgccca tattggcgat gaagaacgac tgcgtgacgc
    42241 ccggccgggt ttgcacggtg acgatctcct gcgccccggc gcacgcggcg aatcccaccg
    42301 ccagaaaagc cagaagcttc aaacgcgctt cgcgaggtcg gcggcgaggc cggtgtacct
    42361 ggcgggagtg agcgcgagca ggcgcttctt ctcctcgggc gggatcggga gcgtcttcac
    42421 gaacgcggcg agcgcgcgcc gctccaggcg cttgccgcgg ctgatggctt tcagcttgtc
    42481 gtaggcgtcc ggcacgccgt ggcgccgcat cacctgctgc accgcctcgg cgagcacttc
    42541 ccagttcgcc tcgagatcgg ccgcgaggcg cgccgggtcg gcctcgagct tggcgaggcc
    42601 gcgcaggcat gcgccgtagg cgagcagcgt atggccaagc gcggtgccga cgtttctcag
    42661 cgcggtcgag tccgagaggt cgcgctgcca gcgcgacacc ggcagcttct cggcgaggtg
    42721 gcgcagcagc gcattggcga tgccgacgtt gccctcggag ttctcgaagt cgatcgggtt
    42781 caccttgtgc ggcatggtgg aggagccgac ctcgcccgcc ttcaccttct ggcggaaata
    42841 gccgagcgag atgtagcccc agagatcgcg atcgaggtcg agcagcacgc tgttggcgcg
    42901 cgcgtaggcg tcgagcagct cggcgagcca gtcgtgcggc tcgatctggg tggtataggc
    42961 gttgaattcc agcccgaggc gctcgacgaa gcgccggcag aagcgctccc agtcgaatcc
    43021 cgggtaggcg gtgacgtgcg cgttgtagtt cccgaccgcg ccgttgatct tgccgagcgc
    43081 cgtcaccgcg gcgatgcgct cgcgcgcgcg agcgagccgg tgcgcgaagt tggcgatctc
    43141 cttgccgagc gtggtcggcg tcgccgcctg gccgtgggtg cgcgacagca tgggcagtgc
    43201 ggcgtgtttc cgtgccagat tcttgagtgc accaatgact tcgtccagct tgggcagcat
    43261 gaccttggcg cgcgactcgg cgatcatcag cgcgtaggcg aggttgttga tgtcctcgga
    43321 agtgcaggcg aagtgaatga actcgagcgc gcgctgcgct tctgcgttct ttgccagctt
    43381 cgatctcagc cagagctcga tcgccttgac gtcgtggttg gtctcggcct cgatgttctt
    43441 gatgtgctcg gcgtcacgct cggaaaagtt cttgaccaaa gaggcgaatg cagtacgggc
    43501 ttggggtgaa aaccccttca gctccttgat cgcgcgctcg gccgccagcg cctgcagcca
    43561 ttccagctcg atgcgggtgc ggtagcggat gagggcctgc tcgctgaagc aggcgcgcag
    43621 gggatcggcg ccctttgagt accgcccgtc gagaggcgac agggcggcga gcggcgatgg
    43681 catggccctc tattataagg gggatgagaa tttcgcttgc cgtggtgagc cgcgaagcgc
    43741 gcgcatgccg cttttgcgaa gacaagctcc cgcaagagcc gaagccggtt tttcgggcag
    43801 gcaagagggc aacgctgctc atcgtcggcc aggcaccggg gcgactggtg cacgagaccg
    43861 gcattccgtg gaacgatccc tcgggcgagg tgctgcgcgc ctggctcggg ctcgaccgcg
    43921 acgggttcta cgacacctcg cgcatcgcca tcgtcccgac cgggctgtgc tatcccggca
    43981 cggtcggcgg cgcagacctg ccgccctgcg ccgaatgcgc gccgctatgg caaccgcgct
    44041 tccgggccgc gctgccggag gttcgattga ctttgctggt gggaagttac gctcaagcgt
    44101 actacctcgg ttcttcccgg aagaaaaccc tgggcgaaac cgtgcgcgcg taccgcgact
    44161 acctgccgga attctttccg ctgccgcatc cgagctggcg aaacctgctg tggctcaagc
    44221 gcaatccctg gttcgcacgg agcgtcatcc ccgcgctcag gcgccgcgtt aaggcgtgca
    44281 tctgacctgt ataatccgcc gcctttcatg aaacttctcg cgtcgcccgc cagcccctat
    44341 acgcgcaagg tccgcatcgt cctcactgag aagaaaatcg actgcgacct ggaggtggtg
    44401 gacgtcaatc cggcggagaa ccccgtgaat cggcacaacc cgctcggcaa gatcccgacc
    44461 ctcatcctcg acgacggcac ggtgctctac gactcgcgcg tgattgtcga attcctcgac
    44521 ggcaagtcgc cgatcagccg cctgatcccg gaggacctgc gcgatcgcgt cgcggtgcgg
    44581 cgctgggagg cgctcgccga cggcgtgctc gacgccgggc tgctggtgcg ctacgaggcg
    44641 atgcgcgacg caagcgagcg tagcaaggcc tggagcgaca aacagctcgc gcgcgtgaag
    44701 cgcggcatgg cgcagatgac gagcgagctc gccgagcgcc cttggtgcca cgccgagcgc
    44761 tactcgctcg ccgacatcgc gcttggctgc tgcgtcggct ggctcggctt ccgcaagccc
    44821 ggcgacgtcg actggcacgc cgaatacccg ccgctcgcgc gccactacga aaagctgatg
    44881 acgcgtcccg ctttctccga gaccgtgccc caagttccca agtaaagaca aaacagagac
    44941 aaaacaggga cagaccacgt tttcggcagg tcgaccactc ggaaaacgtg gtctgtccct
    45001 gaatcagctg ttgatgactc ctccaccgag acagatctcg ccgtcgtaga gcaccaccga
    45061 ctgccccggc gtcacagccc attgcgggct ggagaaatcg acccgaacct cgctttccaa
    45121 caccttcgaa agcgtgcacg gcgcgtcggc ctggcggtaa cgggtcttcg ccgtatgggc
    45181 ggacgccgtc gcgggagcat tgccggacac ccatgccgtg tccgccgccg acagcgtctt
    45241 cttcatcagc agcggatgat cgtggccctg caccaccacc agctcgttcg cagcgatccg
    45301 cttgtcggcc acgtaccacg cctcgcctgc cccaccgatg ccgatgccct tgcgctggcc
    45361 gatcgtgtaa aacgcgagcc ccatgtgctc gccgacccgc ttgcccttgt cgtccacaat
    45421 cgcgcccggc gctttcggca gatagcggtt gagaaattcg cggaacggcc gctcgccgat
    45481 gaagcagata ccggtcgagt ccttcttggc gtggttcggc agcccggcct cgagcgcgat
    45541 gcgccgcacc tcggtctttt tcagcccgcc caccggaaaa atcacgcgcg agagttgctc
    45601 ttgcgtgagg cgatgcagga aatagctctg gtctttggtg ttgtcctttc ccctcatgag
    45661 gctgaagaca ttgccttcag aaaccactcg cgcgtaatgc ccggtcgcga tcttctcggc
    45721 gcccaggcgc atggcatggt cgaggaaggc gcggaacttg atctccgcgt tgcacaggac
    45781 gtcggggttc ggggtgcggc cggcggcgta ctcgcgcagg aattccgcga acacgcgctc
    45841 cttgtactcg gcggagaagt tgaccgcctc gagctcgatg ccgatggtgt cggcggcggc
    45901 cgcggcgtcg accaggtcct cgcgcgtcga gcagtactcg tcgtcgtcgt catcctccca
    45961 gttcttcatg aagaggccga cgacctcgtg gccggcgcgc ttcgcgagca gcgcggcgac
    46021 cgccgagtcc accccgcccg acatgcccac cacgaccttc acgagaactc cggcgaaaaa
    46081 acgtcgagcg ggaaccggcg cccggcgagg aaatcgtcga cgcatttctg caccaggggc
    46141 gagcggtgct ccgctttgcg ctgccggagc tcctcgggcg tgagccagtg gagcgccacg
    46201 atctccttgt ccaggggccg ctcgacggtt ccggcaagcg tcccgcggta gcagaaccgc
    46261 aggaaggtga agtccttcgc cggatcgcgc cagcggtaga tgccgaccag cgcggtcggc
    46321 tcgaagcggt gcgcggtctc ttccaggacc tcgcggctgc acgcggcggc gagcgactcg
    46381 cccggatcga ggtgcccggc cggctggttg agcaccaggc ggcccgaaat acgctcttgc
    46441 accaggagaa acttgccgtc ccgctcgatg accgcggcga cggtcaccga gggcttccaa
    46501 atcgccataa cgtcaaatgc ggctcatgcc gctttcttgg tgttcttgcg gatgaagtcc
    46561 gacatccgct gcacgccggc ctcgatcgcc tgcttcgaag cggcatagga aaagcgcagg
    46621 tgctggccct cgccgggcac gcgcgtgccg aaatggatgt ccgcgagcac cgcgacgccc
    46681 gcctcgtgca gcagccgctt cctgaactcc tcggagtcgg cggcgccgat gcggcggcag
    46741 gcctcggtga cgttcggcca gacgtagaac gcgccgcccg ggatctggca cttcacgccc
    46801 gggacctcgt tcagcaggcc gacgatgaag tctcttcgct ccaggaagct cttcttcatc
    46861 gccgccgccg cttcctgcgg gccggtgatc gcttcgaccg cggcccactg gctgatctgc
    46921 gaggcgcacg actcggtgtt ggtgatccag cgcgtgaaga cgggcgcaag ctgggggttc
    46981 gaggtgaagc cgatgcgcca gccggtcatc gcccaggtct tcgaggcgcc atccgagatg
    47041 atggtgcgct cgtacatccc cggcacctgg gcgatcgaga aaaactcgct ctggaacacg
    47101 aggcgcgaat agatttcgtc cgcgtacacc cagacctgcg gatgcttctt cagaaccccg
    47161 gcaatcgttt cgagctcgtt gcgcgtcagg cagccgccgg tcgggttgtg cgggtagttg
    47221 aggatcagca gccgggtctt tttcgtgatc agacgctcca gctcggccgg atcgaacgaa
    47281 aagttgcggt tttcgtgcag gtggatcggc accggcttcg cgccattcgc cacgatctgc
    47341 gactcgtaga tcgggaagcc ggggttcgga tagatcacct cgtcgccggc gccgtggtcg
    47401 gtggtcgaca ggatggcgta ggcgatgaag ggcttcgcgc cggcgccgac caccacgtcc
    47461 tcggcgcgga tcggcaggcc gccgcgcatc ttcgcgaggt atttcgccgc ggcttcgcgc
    47521 agctcgtcga tccccgcgga gggcgtatac ccgtgcttgc cgcccttgat ggcgcggatc
    47581 gcggcctcct gcacgttgtg gggcgcagga aagtccggct ggccgatgca gaagctgacg
    47641 atgtcccggc cctggcgcgc gagcgcgttc acctcggcca ggacgacgaa ggcgttctcg
    47701 gttcccaggc tattcgcccg gcggctgatt tcaggcataa ggacccttat tatgccgtcc
    47761 catgctcgtc aattgcgtgg cctacgaggg cggccgcaag ctcgccgaca tcgagaagcg
    47821 cgagatcagc aactacctcg cgcggccggg ctgcttcgtg tgggtggcgc tgcgcgacgc
    47881 gaccgaggcc gagcttgccg agatgcagga ggaattcgac ctgcacccgc tcgcgatcga
    47941 ggacgcccgc gtcggccacc agcggccgaa gatcgaggag tacggcgact cgctgttcgt
    48001 cgtgatgcac acgatcgagg tcgagggcga cgacctcagg gcgggcgagg tgcacgtctt
    48061 cgccggagcc aactacgtgc tctcggtgcg ccatcgcacc gagcgcggct tccaggacgt
    48121 gcgcgcgcgc gccgagcgcg agcccgacct cctcagccac ggctcgggct acgtgctcta
    48181 tgcgctgatg gacgccgtcg tcgatcgcta cttcccggtg ctcgacgcgg tcgagatgga
    48241 gctcgagaag ctggaggggc gcctcttcac gcccggcgct tcgccgcgcg agaacatcga
    48301 gtcgctgtac tacgtgaagc agaagctcac caccatgaag cacgccaccg cgcccctgca
    48361 ggagaacgcc ggcaagctct acggcgggcg cgtcccgggc gtgtgccatg ggctgggcga
    48421 atatttccgc gacgtgtacg accacctcgt gcgcatcaat cagtcgatcg acgcggctcg
    48481 cgagaccgtg aacaccgcca tccaggtggc gctcgcgatg gtggccaccg gccagggcga
    48541 aatcacgcgg cgcctcgcgg cttacgccgc gctggtcgcg gttccgacca tgatcgccgg
    48601 cgtctacggc atgaacttcg agcacatgcc ggagctgcag tgggagctcg gctacccgct
    48661 caccatcgcc gcgatggtgg tgctcgacgt ggtcctcttc tggcgcttcc ggaaagcggg
    48721 ctgggtctga acccggccta gggggtttcc ttcaggaccc cggccaccgt gccgaacatc
    48781 tggtcgaggt gctttttctc ggcgatgagc ggcgggcaga aggcgagcga atcgcccacc
    48841 gggcgcacga acacgccctt gtcccagcat ttctcgaata gctcctgcgc gcgcacgccg
    48901 gaggccgcgc cggggcgcgg ggcgagctcg accgcgccga tcaggccgag gttgcggcaa
    48961 tcgaccacgt tcggcaaacc cttgagggag tgaatgccgt cttcgaagta tttccccagg
    49021 gtcctgggct tttcgaacag cttttcttcc tcgtaggtgt cgagcgcggc gattcccgcg
    49081 gcgcaggcga gcggatgccc ggaataggtg tagccgtgga agaactccac ccccggcact
    49141 ttcccgctgg tgaaggcctc gtagatcgag tccttgacga tcacgccgcc cagcggcacc
    49201 gcgcccgagg tgatgccctt ggcgtaagtg atgagatcgg gctcgatgcc gaagtgctgc
    49261 gccgcgaacg gcgcgccgag ccggccccag ccggtgatca cctcgtcgaa caccagcaga
    49321 atgccgtggc gggtgcaaat ctcgcgcagg cgcttgaggt aacccttggg cggcaccagc
    49381 accccgcccg aagcggaaac cgggtcgacg accaccgcgg cgatgttcga cgcgtcgtgc
    49441 agcgtgacga tgcgctcgag ctcgttggca agctccacgc cgttcggggg ttcgccccgg
    49501 gaaaaggaat tcccctcgat ctcatgcgtg tgcctgagat ggtcgatacc tggcaaaagc
    49561 gcaccgctgt acgccttgcg gttcgccggc acgccgccaa gcgacatgcc ggcgatgttg
    49621 acgccgtggt aggagcgctc gcggccgacg aagcgtgtgc gcgtgccctc gccgcgcagg
    49681 cggtggtagc cgatggcgat cttcatcgcg gtgtcgatcg cctccgagcc cgagttggtg
    49741 aagaacacgt ggcccatgcc cttcggagcg agcgcggcga tgcgctcggc gaggcggaag
    49801 gcgagcgggt gcccgagcga gaacgaggcg gcgaagtcga gctcgcccgc ctgccggcgg
    49861 atcgcctcga cgatgcgcgg ccgggcgtgg cccgcgttca cgcaccacag cgtcgccagc
    49921 ccgtcgagca cctgccggcc ctcggcagtg gtgaagtgca tgtccttcgc cgcggtcacg
    49981 agcttgggct tcgccttgaa gcgccggttg tccgtgaacg gcatccagta gtgctcgagg
    50041 ttgagcgcgc tggcggcgga gaggtcgcga gggttcatag gctctatttt gacagccgcg
    50101 ctagagccta agttcggcgc gcaacaaatt ccatgccacg gccgccaagg cgtccgcgcg
    50161 ccgctcgacg agtccaattc gtgccaattg cgtcacatcg gtgtgcacgt tcttgtaatc
    50221 gcgctgcaac cgtcgggcca gctcgtagat cgacatcgcg ccgtccgcgt gaagtttttg
    50281 caacaacgtc cagcgagcag gggatagcgt ccgcaggagc aggggcaggt cagagaagct
    50341 caggatatcg agcggccgca tggcccggcc ctcggccacg cgattccacg ccgcctcgaa
    50401 gcgatcgagc gcgtcggccg ccgcgccgat ccggatttca agctttctgg acttcgcgct
    50461 cgaagtcata gcggagctgc tcgaccgacc tgaagtcgta cttccgcctg gagtcgtacg
    50521 tgacgccgcg ccagcgcagg cggtagcgca agtcgtcgtg cagctcgatc tcgaggatcg
    50581 agccctcgag caccaccgtt tcccgcagca gcaacatatg gcttatcgta ccatatatga
    50641 gcaggcgtaa aattagcaca tgaaggactt tttcagcctg taccgccacg ggtttgcgcg
    50701 cgtggcggcg gcgacgccgc tggtccgtgt cggcgatccg cagtacaacc tcgacgccac
    50761 ggtcgagctg atgcgcgagg ctgcgcgcga gaaggcggtg ctcgcggtgt tccccgagct
    50821 cgggctctcg gcctacacct gcgatgacct gttccaccag caggcgctga tcgagggcgc
    50881 ggaggaagcg ctggccgccc tgataaagag ctccaagaat cttccgatag ctgcgctcgt
    50941 gggcctgcct gtcgccgtcg acggcctgct gtacaactgc gcagcgctcg tctgccaagg
    51001 acggctggtc ggcgtggtgc cgaagatcta cctgccgaac taccgcgagt tctacgaggg
    51061 ccgccagttc acgccgggcg acacggcgag gcgaagcgag atccgcctcg cggggcaggt
    51121 ctgcgctttc ggaaccgacc ttctgttcaa ggcggccgag ctgccaaagc tggtccttca
    51181 cgtggagatc tgcgaagacc tctgggtccc ggcgccgccc tcctcgttcg ctgcgctcgc
    51241 gggcgcaacc gtgatcgcga acctgtcggc gtcgaacgtg gtgatcggca aggaaggcta
    51301 ccgccaccag ctcgtcggca accagtcggc gcgctgcctc gccgcgtacc tctacagcgc
    51361 ggcgggcctc ggcgaatcga ccaccgacct cgcctgggac ggccacgccg tgatctacga
    51421 gaacggcacg ctgctcgccg agtcgcggcg tttcgccgac gcgccgcagc tcgcggtggg
    51481 cgacgtcgac ctcgatcgcc tgctcgccga ccgcatgcgg cagaacacct tcggcgcttc
    51541 gatgcgccgg catgcggacc gggctttccg gaccgtggaa ttttcgctgc cgctcccgaa
    51601 gggaaacgtc gccttggaaa gaaaaatcga gcggctgcct tacgtgccgg gcgacccgaa
    51661 gacgcgcgac gcgcgctgcg aggaggtcta ccgcatccag gtgcaggggc tggtgacgcg
    51721 catgcgcgcc accgggacga agaagctggt gatcggcgtt tccggcgggc tcgactcgac
    51781 gcaggcgctc atcgtgtgcg cgagggcgat ggacgagctc aagctcccgc gcgccaacat
    51841 cctcgcctac acgatgccgg ggttcgcgac ttccacgcgc accaggagcc aggcctggca
    51901 gctcatgcgc gcgatcggcg ccaccgccga ggaaatcgac atcaagccct cgtgcatgca
    51961 gatgctgaag gacatcggcc acccgttcgc caagggaaag aaggtctatg acgtcacgtt
    52021 cgagaacgtg caggcggggg agcgcacgag ccatctcttt cgactcgcca acttccacgg
    52081 cgggatggtg gtgggcaccg gcgacctgtc cgagctcgcg ctcggctggg cgacctacgg
    52141 cgtgggcgac cacatgtcgc actacaacgt gaacgcgagc gtgccgaaga cgctgatcca
    52201 gttcctggtg ggctgggtgg gcgagtcgaa cgagttcagc gccgacgtga agcgggcgct
    52261 ccgccgtgtg ctcgagaccg agatcagccc ggagctggtg ccgggccggc agtcgagcga
    52321 ggcaaccgtc gggccctacg agctgcagga cttcaatctc tactacatcc tgcgcttcgg
    52381 ctattcgccg gcgaaggtgg ccttcctcgc gtggcacgcc tggcgcggca aataccgcct
    52441 gcccgagatc cgcaagtggc tcggggtgtt cctcaagcgc ttcttccaga accagttcaa
    52501 gcgcagcgcg gtgccgaacg cgcccaaggt gggctcgggc ggctcgctct cgccgcgcgg
    52561 cgactggcgc gcgccgtcgg acggcagcgc aaacgcctgg ctcaaggacc tcgcgcgggt
    52621 cccaacggcg gaataagtcc agaagttttc aagacttcac cattgcgcgc agcacaacgc
    52681 gggtcaaatt ttgcgcgcgg tcaaccggcg gtaacgcggc ggcgttaggc tgattgacat
    52741 gaagccgcac tcatccaacc tggaggcgct gctcaacacg gcgccgagcg ccttcgccgg
    52801 cggcgacctg cccgtgctgc cgaggatcaa gtcgctcgcg gccctgaggg tggaagtgag
    52861 gcggcacttc ggcaaactct cgaaccagga gctcgacgct ctcgccgcct ggttcaaccg
    52921 gaatttcttc cggctcgaac gctagtccct acttacttca tgtacgccgg cttgtaggcc
    52981 cgcccgcggc gcttgccgcg caccggcgag cccttggcgg ccttcgagcc gtagttcttg
    53041 ctgttgtcct tcatctcggc ggcgcgttcg cgcatgcgct gctcggcgag ggtttcccgc
    53101 gcgagcgcgg ctccgtactt gcgtttcatg gcttccctat cctctggtgg tcgtcttgcg
    53161 gatttccttc tcgtccgacc actcgagaag tccgttgcgg atgttccata gctgcaggtt
    53221 gagcttgtag tacacgtcct tcgtgtcctt cacctgcttg acgatggaga tgatgttgcc
    53281 ttcgagccgg tactgcgcgc cgaccatgcg ccccatctcg gccgcgctgt ccttggcata
    53341 gagctcgctc tgctgccgct tgagctcctc ggtctgctgg cccatgccga ccgcgtcgac
    53401 cgcgaagcgc acccggccgc ccttctgcag ctcggaacgg atgctgtcgg tgatggcgcg
    53461 cgtgtcgatg tactcgctgg tcttgttgcg cacttcctgc accgtgagga tcggcaggtt
    53521 ggcgctggcg atcgccggcg cctgctgcat cgagcgcgcc atcgactcgg ccatcatctg
    53581 caggtcggtc gagccgtact ggttggtcag ggtttcgacg cccttggcat cgccgtaccg
    53641 ggtcttttcc tgagcacaac cggccgccag ggcgagaatg gcggcgagcg cgcccagcgc
    53701 g
//
LOCUS       DATBSZ010000035        25582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_140447, whole genome shotgun sequence.
ACCESSION   DATBSZ010000035 DATBSZ010000000
VERSION     DATBSZ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 25582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25582
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140447"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VIG70_02390"
     CDS             <1..1122
                     /locus_tag="VIG70_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8249427.1"
                     /translation="FDWPSFDEKTAACLCYTSGTTGNPKGALYSHRSTMIHAYAAALP
                     DTLNLSAREVVLPVVPMFHVNAWSLPYSCTMTGAKMVFPGPHLDGRSLHELFEAEGVT
                     MSAGVPTVWLGLLTYMKEKNLRFSTLKNVVIGGSACPPAMIRAFQDDYGVQVLHAWGM
                     TEMSPLGTAGTLKAAQLKLSKEERYSLQNKQGRAIFGVDFRIVDENGAELPWDGRAFG
                     DLQVRGPWVIKSYFKGEGGDPLREGWFPTGDVCTIDPEGYIQVTDRSKDVIKSGGEWI
                     SSIDLENIAIAHPAIAEAAVIGVRHPKWDERPIVVAVKKAGKEISRDELLRFYEGKIA
                     KWWMPDDVVFVNELPHTATGKLSKLTLRQQFKDYRLDPK"
     gene            1109..1867
                     /locus_tag="VIG70_02395"
     CDS             1109..1867
                     /locus_tag="VIG70_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY8249428.1"
                     /translation="MIRSETKDRVARVRFDRIEKKNAITVEMYPMLGNALAAADADPQ
                     VRAVLLHGTAECFTAGNDVHDFVNVPRQPGTSPAQALFKVLPHMKKPVVAAVGGPAVG
                     IGTTLLLHCDLVYAAPNARFQLPFVPLGIVPEFGSTFLLPLLAGYQRAAELLLLGQPF
                     TAQKAYEAGIVTAVVPQEALLAEAEKAAMALAALPPESVRLTKRLLKSRYTEPLAAAI
                     DEETRIFTERLSSPEAKEAMSAFLEKRKPDFSRF"
     gene            1891..2601
                     /locus_tag="VIG70_02400"
     CDS             1891..2601
                     /locus_tag="VIG70_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY8249429.1"
                     /translation="MSILLIGFSAHADLYRWVDRETGSVKFSNTPPPWFGDPDRERSA
                     PAVEIVPYRGPGTPPRSAATPEVSPETARLVASLETHWATLVRFFAALPPTTDFRPGT
                     GMPQQIDAYQAVSAELDRLDPAGTPRRRAQEAGIFETLRRAREAQVSAKPPAKPPASP
                     PSTQAEARWRQALQQMAALAERPEPARSMPELQDALRTLEALHVELNQLDPSGAAARE
                     REVQATLEGVRRSFQAVR"
     gene            2598..3056
                     /locus_tag="VIG70_02405"
     CDS             2598..3056
                     /locus_tag="VIG70_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY8249430.1"
                     /translation="MSPRALSVRDLESRVGQEVGVSPWVEVTQERIDTFAKAIDDFQW
                     IHVDRARAQASPFGGTIAHGFLTLSLLSHLSERTFGFSDRKMGVNYGLNRVRFTAPVP
                     SGSRVRARFTLHKFEKLADGGVQVTWNTVVEREGADKPVLVAEWLGRHYY"
     gene            3146..4186
                     /locus_tag="VIG70_02410"
     CDS             3146..4186
                     /locus_tag="VIG70_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY8249431.1"
                     /translation="MTERWKNKPAGSNWGEFGPDDQRGRMNLVTREKVLQGIAEVREG
                     LTFCLSLPLDYPGGSVLNPRRSPPRLASTVRSNKQNFCYPLADENPDLTDVVCDDVVL
                     LTLQYSSQWDSFAHVGSRFDADGDGKAEIVFYNGFRAGEHVVPATAKKTEGWDRFEGT
                     EARALGIQNLAEHGAQGRGVMIDLHAHFGRERRPVGYDDLIKVLEKDKVVIERGDMVC
                     LYTGFADVILELKKNPDPKLLHGTCSGLDGRDEKLLQWISDSGLACLISDNYAVELIP
                     TSLAKPKPHAAMPLHEHCLFKNGIHLGEMWYLTELARWLRAHKRSRFLLTAPPLRLPG
                     AVGSPPTPVATV"
     gene            4183..4893
                     /locus_tag="VIG70_02415"
     CDS             4183..4893
                     /locus_tag="VIG70_02415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8249432.1"
                     /translation="MKVALVTGGNSGMGEATVKELAKRGWQVAAMARSKVEKEFRGDV
                     AEDADCRRVAKAVLDKWGRIDALVNSAGTTKFVAHADLEGLSADDILGIFRVNLVGPF
                     QMIRACAAALKASRGCVVNVSSVASILGTGSSIAYAASKAALDTMSFSLARALGPEVR
                     VNVVAPGYVRTPWQVAAHGADGAADLERRFAERAPLKAAAEPQDVADAIAWLVEGARR
                     VTGEIVYVDGGMHIASPR"
     gene            4890..6413
                     /locus_tag="VIG70_02420"
     CDS             4890..6413
                     /locus_tag="VIG70_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8249433.1"
                     /translation="MIYDYVIVGAGSAGCVLASRLSEDSSIKVLLLEAGPRDTDFWIH
                     VPLGYGKLFARTDLNWAYESEPEPALNGRRIFTPRGKVLGGSSSINGLVYIRGQPEDF
                     DGWRIPGWDFASLAPYFEKAESQLKVSDLERHELCDAFIASAMALGIPRNDDFNGAAQ
                     EGTGYYRATAYRGRRRSTATAYLRPAEKRPNLRVEVGALATRILFEGKRAVGVSFRQG
                     ERSHMVRAEKEVLLAGGAFNSPQLLELSGVGPRELLEKHGIAVVHDAPKVGERLQDHF
                     YVRTFWRSRRPITLNDDMASLWRKARIGLQYLLKKTGPLTVSAGHAAAFVRTKPWLRR
                     PDAQIYFINFSTAKRGGVLHAHSGFTCAVSQLQVESRGSVHIRSADPAAPPAIRYNYL
                     AAESDRRVMVEGLKVIRRICATPPLRDYVAAEYLPGEKVVTDEDWLAYCREMGETVFH
                     PTSTCSMGAVVDEKLNVKGVQNLRVIDASVMPAVPSGNINAAVIAVAEKAAALITAQ"
     gene            6418..6726
                     /locus_tag="VIG70_02425"
     CDS             6418..6726
                     /locus_tag="VIG70_02425"
                     /inference="COORDINATES: protein motif:HMM:NF023171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HEY8249434.1"
                     /translation="MWEGAAIVLIAAGIFLWLDSLRARERAVRAGRAACERYALQFLD
                     DTVSFTRIRLGRDPDGQLKIARTYTFEFSDTGNNRRHGAIVMLGGELQDLQLEPYRVQ
                     "
     gene            6736..7464
                     /locus_tag="VIG70_02430"
     CDS             6736..7464
                     /locus_tag="VIG70_02430"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEY8249435.1"
                     /translation="MTSKWLESRVIENRRWTDALFSLRVEGPQLGFEAGQFVRIAVDE
                     RIARPFSFVNAPDDPVLEFYGIVVPEGPLSPKLMQLRAGERLLVAPNPAGFLVLSEVP
                     DADTLWLVSTGTGIAPFLSILRTETPWRRFRNVVLVHAVRHARELVYQDMIRKIVKEK
                     SLRYLTFVSREAASGSLTGRIPAAMRDGRLETAAGLALNQHSHVMLCGNPDMLKDAAG
                     ALAERGLRKHRRRTPGHITVESFW"
     gene            7524..8294
                     /locus_tag="VIG70_02435"
     CDS             7524..8294
                     /locus_tag="VIG70_02435"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HEY8249436.1"
                     /translation="MRFAYENADAYLRWKAGSYLDLQGEEADELDDRIDEFHAWHRRH
                     ALPKYVGLAHEASQRFSDGLSRPDLVWGYDSLRVQARESLRKAGDLVAPLLDRLTPEQ
                     VAQIERRFAEENRQFYRDNLRAPERDRRKKRAKQTVERLEDWVGKLTKAQIQRVREYA
                     DRTPMVDELRDRDRKRVQKDVIAILRAREARARLGERLAHWDRGREAVYVASLDLWRD
                     QYFAMLMDIDRSLTPEQRARAQGHLRRYAEEFEALASR"
     gene            8291..11983
                     /locus_tag="VIG70_02440"
     CDS             8291..11983
                     /locus_tag="VIG70_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8249437.1"
                     /translation="MNDPKPLRSFVEPADARVADPGAYAGRTRYTQLEYEALLANLSI
                     GIAFTRERCFFLCNPKFAEMFGYESSELIGMPGEAVYPSRESYLALGQIAAPVLSAGR
                     QLDLEWEVRRKDGSTFLCRLVAKSIDPANPQHGTVWIVEDITDRRRHADEIARLLREQ
                     DAILGTASVGIVFVRDRRLVRCNRRYEEMYGYGPGEMDGQPAWIIYPGTVEFEEAQSV
                     YEALARGQTSRRIGLRRRKDGSTFWNRADGRAVDPQNPHKGSVWTVEDITEQRRAEDE
                     LQRVLAEQQALLDNVVVGIAISRERRVMRCNRRFEEMFGFGPGEANGASWRQVYFTED
                     EFDLRAQLYVELDQGRTHAREQWLRRQDGSGFWCRISGRAVAAGDPGRGYVWLMEDVS
                     ERRRADESLERLVREQDAVLQNAVTGIIFVKDRRIVRSNRRFEEIFGYGAGELIQRST
                     RFMFATREEYEAGGETLYEPVWRGETVFIERRHVRKDGAQIWCSISGRAVQPGDPAQG
                     SVWLFDDITQEHESEERVQRALAEQELILDNAAVGIAFVRNRAIQRCNRFLEDMVGAG
                     PGELIGQSTATLFASREEWEDAGRRTYTATPPGETFEGEARFRRRDGSTFQCRARGRR
                     TDTGEAEQEWIWSYEDVTAEREAEMRVQRALAEQDLILDNATVGIAFVRSRSIARCNR
                     FFEEMMGAKRGALVGARTSALFADEKDWEEAARRAFEETPPGGIHRSEARLKRADGSS
                     FICAMRGRRIDSGDAEQVWIWSYEDVTAEREADLRVQQALGELERAVAERTAELEEAK
                     ARAQHLADHDALTGLPNRRLLEDRLTQALALGHRNRKNSAVMFVDLDRFKMINDSLGH
                     AVGDALLKEVAGRLVTQLREGDTICRIGGDEFVVVLPEVKRSSDVAHVAQKVIEQLSQ
                     PVTVEERELVVTCSIGIAVYPDDGRDAETLIRNADAAMYHAKELGRANYQFFTEQMNQ
                     AASRRLQLESDLRRAVGKGELRVHYQPIVDVASGHIAAHEALVRWQHPERGLVLPAQF
                     IQLAEETGIILKLGEWILGEACRWNTFIGARSHDGGELQIAINLSARQFNDPHLSKVV
                     ARALKETGLPPRLLELEITESTAMQQTDVTLRTLKKLKQLGVSIAIDDFGTGYSSLSY
                     LRRFPVDKVKIDRSFVAEVPANRDQGAIVSAIIALAHALQIKVVAEGVETEAQREFLK
                     SCGCDLIQGYLVGKPADADSAARDYV"
     gene            12009..12758
                     /locus_tag="VIG70_02445"
                     /pseudo
     CDS             12009..12758
                     /locus_tag="VIG70_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(12709..13350)
                     /locus_tag="VIG70_02450"
     CDS             complement(12709..13350)
                     /locus_tag="VIG70_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8249438.1"
                     /translation="MVLLLAALTLVAPAAFPEPPKSRTEALAALKNTDTAARAEAIVW
                     VANHGSMADAPLLHERLRDESSLVRQFAEQGLWLLWSRSGDPELDRLMARGAEAMQAG
                     RHGDAIGLFSVVIDRKPEFAEGWNRRATVYYLAGEYDKSIADCLEVLKRNPRHFGALS
                     GMGQIYFQLEDWGSALGWYRRALEVNPNMLGVEMNIRLIEEKLKDPPAPGRAT"
     gene            13416..13877
                     /locus_tag="VIG70_02455"
     CDS             13416..13877
                     /locus_tag="VIG70_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249439.1"
                     /translation="MKALFRPFILAGLLASGSVLAAGDPVTTSAPKDPVLEKVSAATA
                     RKDWAGAAAVLRDAVSADPNNANYHNLYAYSIRKGVNPDMNLVFKHYNEALRIDPQHR
                     GAHEYIGEAYLMVGNVGKAKEHLTQLDKLCFFPCVEYTDLKKAIAEHETKR"
     gene            complement(13882..14787)
                     /locus_tag="VIG70_02460"
     CDS             complement(13882..14787)
                     /locus_tag="VIG70_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8249440.1"
                     /translation="MRLNQIRDFIVTVEAGSLRAAARSVGVSQPALTKSLRQLENELG
                     VQLLQRSSRGVSPTRSGHAFLTRARVVQAELRRATEDLADLKGERAGAVAFGVSPSAA
                     VLFVPEAHVRFRQKYPFAAVRVVEGMLQGLLPLLRDETLDFAVGQKPPGALDPAIRHR
                     PLCRARLVVAGRRGHPLRAAHSLRELADASWLMYSPRGQGGILERFFSAAGLPGPKVV
                     VHCESYAAALALLARTDTLGLVVPQLLAQPYAHGFLQQIEIAETAPDLPVGLFARANA
                     PLPPAAAAMAAAVTAAARRLARVNR"
     gene            14864..15814
                     /locus_tag="VIG70_02465"
     CDS             14864..15814
                     /locus_tag="VIG70_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249441.1"
                     /translation="MRTLLAILALALAQAAAAQAFPSKAVRLVNPFPPGGPLDVLGRI
                     LSERLGASLGRPVLVENKPGAAGNIGAAEVAKAPADGHTVLLTLFSIITSNPHLYDRL
                     PFDPLKDFAPVTALASSDSVLIVHPALPAASVKELIAHAKANPGKLNYASAGKASPGH
                     LTAEFFARRSGAAFTHVPYKGNAEAIRSVLAGETQMMFTPTTGALPLIKAGKLKAIAV
                     YLTERVDELPEVPSLEGFDQKTLPFWYGLLAPAGTPREIVMRLNAETVKALKDPQVNK
                     TLRAGRIFPIGNTPEEFGAMMREGHAAWGRVIRETGVKGE"
     gene            15798..17060
                     /locus_tag="VIG70_02470"
     CDS             15798..17060
                     /locus_tag="VIG70_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY8249442.1"
                     /translation="MSKASRPSFVFILADDLGYADLGCYGGRGSCSPNLDRVAAEGLR
                     LTDGYANSPVCSPTRFALATGRWQYRLRGGADEPIASRNRGSAVLGLPPAHPTLASLL
                     RDAGYATALVGKWHLGFPPHFSPLKSGYQEFFGPMSGSVDYFTHCDSAGVHDLYQGEK
                     EVHRTGYLTDLLSDEAVSFVKKAKGKPFFLSLHYTAPHWPWEGRADRDESKRIRTISH
                     FDGGSVKTYLEMIRAMDEGIGRVMQELPGNTCVAFTSDNGGERFSDSWPLVGGKMDLL
                     EGGIRVPYVVRWPKGAILAQLAITMDWVATFLDAADVAAHPDYPLDGVSLLKESRGRE
                     LFWRMKFRDQKAMRSGKWKWLSIEGNEFLFDLSNDARERANMAKRQPATFEELRKRYA
                     VWDASLPPIPDDAKVSLIGGPQEMAKPS"
     gene            complement(17123..17311)
                     /locus_tag="VIG70_02475"
                     /pseudo
     CDS             complement(17123..17311)
                     /locus_tag="VIG70_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193068.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
     gene            complement(17308..18870)
                     /gene="ggt"
                     /locus_tag="VIG70_02480"
     CDS             complement(17308..18870)
                     /gene="ggt"
                     /locus_tag="VIG70_02480"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY8249443.1"
                     /translation="MAPSFATHGMVACPHALASAAGVDALRSGGSAVDAAVAAASSLA
                     VLYPHMCSIGGDAFWLIYDAKARKVSYLDGGGRAAAAAMLERFAGQAEIPFRGLAPAT
                     LTTPGAVASFCEAHARHGRLPFARCLESAIRYAADGFPVSARVSRWIEQTAPEMDAAT
                     AAIFLRGGPVLKNPKLADTLRTIAARGRAGFYEGEVARELAKLGGFFTERDLAAQGAY
                     WGEPIQGSYRGVTIYETPAPTQGFTVLEMLNLLEPLELSKREFLGPDHVHLLVQAKQI
                     AYNDRDRWLADPRFVEVPIEKLLSKPYADQRRRLIDPARALPWDKVPSEGSLTGDTVY
                     VAAVDAAGNAASLIFSLYGVFGACVTSAATGVVLQNRGAYFSLDPAHPNRLEPGKVPL
                     HTLIASLAFRNEKPWAVLGCMGADGQPQIHVQTYVAMIDFGLDIQQALSAPRWLSGRF
                     GLGEARDTLHIEARYPSATIDELVRRGHPVDRWGEWNELAGHANGITIDGETGILAGG
                     ADPRSDGAAVGY"
     gene            complement(18879..19901)
                     /locus_tag="VIG70_02485"
     CDS             complement(18879..19901)
                     /locus_tag="VIG70_02485"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8249444.1"
                     /translation="MQPLLDHAPMLRSRSFEETRAYLARSHAIDMALADPRGQEFPMD
                     VRLNALFLPGQWVAYLKYGAALRLRLSRRSSPWLARESERRQPPAVYWVHFSLQGDMQ
                     ALVGGQTCDGDEDRGIVLSPTSDNTLHTSNGNARLSLAVREEALNRHLAAMLGEAPRQ
                     AVEFAPSIDATSGHGRRLAGILHWAAAESERGASLGEPLVAARFEAFVLDALLLFQPH
                     NYSEALRRVERRVAPRDVRRTLDFIHANLAQPLTLADLVAVSGVPGRTLLQHFRNFHG
                     VSPMRYLRNLRLQRVRDEFASGTATQVAACALHWGFAHPGRFAIEYRSRFGESPSVTL
                     ARSRKQ"
     gene            complement(19973..21037)
                     /locus_tag="VIG70_02490"
     CDS             complement(19973..21037)
                     /locus_tag="VIG70_02490"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY8249445.1"
                     /translation="MFETTIAGSLPKPAWLAETGKLWPAWRTQGTDLERAKRDATLLW
                     LKEQEDAGLDIVTDGEQSRQHFVHGFLEFVEGIDFEQKVKMGIRNNRYDAMVPLVVAP
                     LRLKGRVHQAEARLLRAHSKKKIKITMPGPMTIVDTIADRHYGDKVKLAMAFAGLLNE
                     EARALEKDGVDVIQIDEPAFNVYMDDVKRWGIDALHRAIAGLKCTTCVHICYGYGIQA
                     NLDWKKTLGESWRQYEEIFPALAKSKVDQVSLECIHSKVPVELLKLLKGKDVLVGVID
                     VASDKVETPEEVSQVIQRASKFVPVRHLFPCTNCGMAPMDREIALRKLQALTAGAALA
                     RKKLGKQGQRTVSAKAKRKK"
     gene            21112..21333
                     /locus_tag="VIG70_02495"
     CDS             21112..21333
                     /locus_tag="VIG70_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249446.1"
                     /translation="MFGMVVGTFCSFVMGLFNPYIANKAGPDWMWGGGRNDLLRNLYF
                     RHDGSFRRYGRIALLATLFGGSAALFWVL"
     gene            21369..21707
                     /locus_tag="VIG70_02500"
     CDS             21369..21707
                     /locus_tag="VIG70_02500"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8249447.1"
                     /translation="MRFEVTAPAAISVAECNCSICAKSAYLHLIVPKSRFRLLQGEES
                     VTTYAFNTGVAKHFFCKVCGIKSFYVPRSDPDGVSVNARCLDPGTVTRMEVRPFDGRN
                     WEASIGEKSW"
     gene            21701..21931
                     /locus_tag="VIG70_02505"
     CDS             21701..21931
                     /locus_tag="VIG70_02505"
                     /inference="COORDINATES: protein motif:HMM:NF018369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HEY8249448.1"
                     /translation="MVKLIAAAVVVGLAVSGPALAQRKDCGELKGEIEAKIKKNGVEK
                     FSLDVVDADKQAEGKVVGTCDGGTKKIVYKRD"
     gene            complement(21928..23127)
                     /locus_tag="VIG70_02510"
     CDS             complement(21928..23127)
                     /locus_tag="VIG70_02510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8249449.1"
                     /translation="MLVVFTPFAAGYYLSYFYRYVNAVIAKDLVADFGIAPSDLGLLT
                     SAYFLAFALAQLPLGVALDRFGPRRCTAALFCVAGAGALVFGLARDLAMLSMGRALIG
                     LGVSAGLMGSIKAFTLWFPRERLTSLSGWMIGIGSLGTLSATAPVEALLGPFGWRALF
                     VALAALSVAAAALIFFVVPERALPGQGDPWREQVGVIGRIFARIDFWRLAVPLVICQA
                     TFQALQGLWFAPWLADVHGLDRAAAADYLFFSAAAYMAASLGLGPVSDAAAKRGMAPL
                     RFYQAGMLICAAAFAPLALGASRGALLFLVLFAATSIASIIAYSLLTQLVPAAQTGRV
                     TTASNLLLFGTSFAFQWGVGAILGHWPSAQGRYDPEAYRVAFGLLLAAQAAAAAWLLS
                     APGGAKR"
     gene            23153..23743
                     /locus_tag="VIG70_02515"
     CDS             23153..23743
                     /locus_tag="VIG70_02515"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEY8249450.1"
                     /translation="MKKRLIVGITGATGAVYGVQILKTLRDTDGWESHVILSDAGALN
                     LWHELKMKRKELEKLADVAYHPKDIAATISSGSFLTEGMVIAPCSMKTLAAVAHAHAD
                     DLLSRAADVVLKERRRLVLVPRETPLNLAHLRNMVAVTEMGGIVFPPVPAFYALPKSL
                     EDLVAHTCARVLDLFGVHSAKLARWQGMKGTPEERG"
     gene            complement(23691..24488)
                     /gene="pcaD"
                     /locus_tag="VIG70_02520"
     CDS             complement(23691..24488)
                     /gene="pcaD"
                     /locus_tag="VIG70_02520"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEY8249451.1"
                     /translation="MKAKVNGIEANYEIHGSEGAPWLTFSHSLACSLRMWDGQVAEFK
                     DRFRILVYDTRGHGGSEAPKGPYSLDMLAEDLRQLLAELKIKRTHYVGLSMGGMIGQV
                     VALTDPGVFDRVVLADTGHTQTPETRKQWDERIKTAETKGMQPLVQGTLERWFTEPFR
                     KGKPEIVKKIADLIQSTPVPGYVGCCHAISNLNTTARLKEIKRPVLAIAGEQDAAAPG
                     TRYIGENVPGAKLVMIPQAAHIANIEQAEAFNRALREFLSSPASARA"
     gene            complement(24485..>25582)
                     /locus_tag="VIG70_02525"
     CDS             complement(24485..>25582)
                     /locus_tag="VIG70_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8249452.1"
                     /translation="SIVAARGGKALHLPLRDLPQDKLRDARNVVLMIIDGLGDNYLMR
                     RGAGGELGRRRRASLTSVFPSTTASAITTSYTGRTPLEHGLTGWFTYFGEAGCVAAAL
                     PFRSRGDYLPLARRGVKPEQVYVCGSIFESLADASFVVTYKEIVDSEYNMCHCRGAQR
                     LAYETLDELVAQTEAAVKSGAGRKFVYVYWPVYDMVSHRYGSESAEAFEQLGKIDQAF
                     GKLLARLAGTDSLVIATADHGFIDVAPEESFELPQSLAPLLRLPLCGERRVAYCHVHD
                     PRTFTEKAKEWLGERADVMPSRELVDEGWFGPGTPHPRFAERIGDVTLVMRGRYTVKD
                     WTTGESRHLHIGNHGGTSDDEMLIPLILEAT"
BASE COUNT         4292 a         8554 c         8643 g         4093 t
ORIGIN      
        1 ttcgactggc cctcgttcga cgagaagact gcggcgtgcc tgtgctacac ctccggcact
       61 accggcaatc cgaagggcgc gctctattcg caccgctcga cgatgatcca cgcctacgcg
      121 gcggcgctgc ccgacacgct caacctgtcg gcgcgcgaag tcgtgctgcc ggtggtgccg
      181 atgttccatg tgaacgcatg gagcctgccg tactcgtgca ccatgaccgg cgcgaagatg
      241 gtgttccccg ggccgcacct cgacggcagg agcctgcacg agctgttcga ggccgaaggc
      301 gtgacgatga gcgcgggggt gcccacggtg tggcttgggc tcctcaccta catgaaggag
      361 aagaacctgc gcttttccac cctcaagaac gtggtgatcg gcggctcggc gtgcccgccg
      421 gcgatgatcc gcgccttcca ggacgactac ggcgtgcagg tgctgcacgc ctgggggatg
      481 accgagatga gcccgctcgg caccgccggg acgctcaaag cggcacaact caagctcagc
      541 aaggaagaga gatattcctt gcagaacaag caaggccgcg cgattttcgg ggtcgacttc
      601 cgcatcgtgg acgaaaacgg cgccgagctg ccctgggacg gaagagcctt cggcgacctg
      661 caggtgcgcg ggccctgggt gatcaagagc tacttcaagg gcgagggggg ggatcccctg
      721 cgcgagggat ggttcccgac cggcgacgtc tgcaccatcg atcccgaggg ctacatccag
      781 gtcaccgacc ggtcgaagga cgtgatcaag tccggcggcg agtggatcag ctcgatcgac
      841 ctggagaaca tcgccatcgc gcatccggcg attgccgagg ccgccgtgat cggcgtgcgc
      901 cacccgaaat gggacgagcg cccgatcgtg gtcgcggtga agaaggcggg gaaggagatc
      961 tcgcgcgacg agctgctgcg cttctacgag ggcaagatcg ccaagtggtg gatgcccgac
     1021 gacgtagtct tcgtgaacga gctgccgcat accgcgaccg gcaagctttc caagctcacg
     1081 ctgcggcagc aattcaagga ctaccgtctt gatccgaagt gaaacgaagg accgcgtagc
     1141 ccgggtgcgc ttcgaccgca tcgagaagaa gaacgccatc acggtcgaga tgtacccgat
     1201 gctcggaaac gcgctcgccg ccgccgacgc cgacccgcag gtgcgcgcgg tgctgctgca
     1261 cggcaccgcc gaatgcttca ccgcggggaa cgacgtgcac gatttcgtga acgtgccgcg
     1321 ccagccgggc acttccccgg cgcaagcgct cttcaaggtc ctgccgcaca tgaaaaagcc
     1381 cgtggtcgcg gcggtcggcg gacccgcggt cggcatcggc accaccctgc tcctgcactg
     1441 cgacctcgtg tacgcggcgc cgaacgcgcg ctttcagctg ccgttcgtgc cgctcggcat
     1501 cgtccccgag ttcggctcga ccttcctgct tccgcttctg gccggctatc agcgcgccgc
     1561 cgagttgctt cttcttggcc agccgtttac ggcacagaag gcctatgagg ccggaatcgt
     1621 cactgcggtg gtgccgcagg aggcacttct tgcagaagcg gaaaaggccg cgatggccct
     1681 tgccgcgctg ccgccggagt cggtgcgcct taccaagcgc ctgctgaaat cgcgctacac
     1741 cgagccgctc gccgcggcga tcgacgaaga gacgcgcatc ttcacggagc ggctgtcctc
     1801 gcccgaagcg aaagaggcga tgagcgcctt cctcgagaaa cgcaagcccg acttctcgcg
     1861 tttctagcgg ttgcaatgaa gtatgtcttg atgtcgatcc tcctcatcgg attttccgcg
     1921 cacgccgatc tctaccgctg ggtcgatcgc gagaccggct cggtcaagtt ctccaacacg
     1981 ccgccgccgt ggttcggcga tccggacagg gagcgcagcg cgcccgcggt cgagatcgtc
     2041 ccctaccgcg ggccgggcac gccgcccagg tccgccgcga cgcccgaggt ttccccggag
     2101 acagcgcggc tggtggccag cctcgagacg cactgggcga ccctggtgcg attcttcgct
     2161 gcgctaccgc cgaccaccga ctttcgcccg ggcaccggca tgccgcagca gatcgacgcc
     2221 taccaggcgg tgagcgccga gctcgatcgc ctcgaccccg cgggcacgcc gcgccggcgt
     2281 gcgcaggagg ccggcatttt cgagaccttg cgccgcgcgc gggaagcgca ggtcagtgcc
     2341 aagcccccgg cgaagcctcc cgcgtcccct ccatcgacgc aagccgaggc gcgctggcgt
     2401 caggcgctgc agcagatggc cgcgctcgcc gagcgcccgg aaccggcgcg cagcatgccg
     2461 gagctccagg acgcgctccg gaccctggag gcgctgcacg tcgagctgaa ccagctcgat
     2521 ccgagcgggg cggccgcgcg agagcgcgag gtgcaggcca cgctggaagg ggtccgccgc
     2581 agcttccagg cggtgagatg agcccccgcg cgctctcggt gcgcgatctc gagagtcgcg
     2641 tcggccagga agtcggcgtc tcgccctggg tcgaagtcac gcaggagcgc atcgacacct
     2701 ttgccaaggc gatcgacgac ttccagtgga tccacgtcga ccgtgctcgc gcgcaggcct
     2761 cgcccttcgg cggcaccatc gcgcatggct ttctgaccct gtcgctcctc tctcaccttt
     2821 cggagcgtac gttcggcttt tccgatcgga aaatgggcgt gaactacggc ctgaaccgcg
     2881 tgcgcttcac ggcgccggtt ccgagcggct cgcgcgtgcg cgcgcgcttt acactgcaca
     2941 aattcgaaaa gctcgccgat ggcggcgtcc aggtgacctg gaacacggta gtcgagcgcg
     3001 aaggcgcgga caagcccgtt ctggtcgccg agtggctggg ccgccattac tattgacgca
     3061 aggcaacctg aaaacaggga cagaccacgt tttcgcccat agcacctgct gaaaacgtgg
     3121 tctgtccctg atttacggag ttcggatgac ggaaaggtgg aaaaacaagc ccgcggggtc
     3181 gaactggggc gaattcggcc ccgacgacca gcgcgggcgc atgaacctcg tcacgcgcga
     3241 gaaagtgctg cagggaatcg ccgaggtgcg ggagggactc accttctgcc tctcgctgcc
     3301 gctcgactat cccggcggca gtgtcttgaa tccgcggcgg tcacccccgc ggctcgcctc
     3361 cacggttcga agtaacaaac agaatttctg ctatccgctt gcggacgaga accccgacct
     3421 caccgacgtg gtgtgcgacg acgtcgttct gctgacgctg cagtattcct cgcaatggga
     3481 ctcgttcgcc cacgtcggca gccgtttcga cgccgacggc gacggcaagg ccgagatcgt
     3541 gttctacaac ggctttcgcg cgggcgagca cgtggttcct gcgaccgcga agaaaaccga
     3601 agggtgggac cgcttcgaag gcaccgaggc gcgagcgctc ggcatccaga acctcgcgga
     3661 gcacggcgcg caggggcgcg gcgtaatgat cgacttgcac gcgcatttcg gccgggagcg
     3721 ccgtccggtc ggctatgacg acctgatcaa ggtcctggag aaagacaaag tcgtcatcga
     3781 gcgaggcgac atggtgtgcc tgtacacggg cttcgccgac gtgatcctgg agctgaagaa
     3841 gaatccggac ccgaagctgc tgcatgggac ctgcagcggc ctcgacggcc gcgacgagaa
     3901 gctgctgcaa tggatttccg actccggtct agcctgtctg atctccgaca actacgcggt
     3961 ggagctcatc ccgaccagcc tcgccaagcc gaagccgcat gcggcgatgc cactgcacga
     4021 gcactgcctg ttcaagaacg gcatccacct tggcgagatg tggtatctca ccgagctcgc
     4081 gcgctggctg cgcgcccaca agcgcagccg cttcctgctt accgcgccgc cgctgcgcct
     4141 tccgggcgcc gtcggctcgc cgccgacgcc tgtggccacg gtatgaaggt cgccctcgtc
     4201 acgggcggca attcggggat gggcgaggcc acggtgaagg agctcgcgaa gcgaggctgg
     4261 caggtcgccg cgatggcccg cagcaaagtc gagaaggaat ttcggggaga cgttgccgag
     4321 gacgccgact gccggcgcgt ggcgaaagcg gtgctcgaca agtgggggcg catcgacgcg
     4381 ctggtgaaca gcgccggcac gacgaagttc gtggcgcacg ccgacctcga aggcctgtcg
     4441 gcggacgaca tcctcggcat cttccgcgtc aacctggtcg ggccgttcca gatgatccgc
     4501 gcgtgcgccg cggcgctgaa agcgagccgg ggctgcgtgg tcaacgtctc gtcggtcgcc
     4561 tcgatcctcg gcaccggctc atcgatcgcc tatgccgcat cgaaagccgc gctcgacacc
     4621 atgagcttct cgctcgcccg ggcgctcggc ccggaggtcc gcgtcaacgt cgtggcgccg
     4681 ggatacgtgc gcacgccctg gcaggtggca gcgcacggcg ccgacggcgc cgccgacctc
     4741 gagcgccgct tcgccgagcg cgcgccgctg aaggccgcgg ccgagccgca ggacgtcgcc
     4801 gacgcgatcg cctggctggt cgaaggcgcg cgccgcgtca ccggcgagat cgtctacgtc
     4861 gacggcggca tgcacatcgc ttcgccgcgt tgatctacga ctatgtgatc gtaggtgccg
     4921 gatccgcggg gtgcgtcctc gcgagtcgtc tttctgaaga ctcttccatc aaggtactgc
     4981 tcctcgaggc ggggccgcgc gacaccgatt tctggatcca tgtcccgctc ggctacggca
     5041 agctctttgc tcgcaccgac ctcaattggg cctacgagtc ggagcccgag ccggcgctga
     5101 acggccggcg gatcttcacg ccgcgcggca aggtcctcgg cggttcctcc tcgatcaacg
     5161 gcctggtcta catccgcggc cagcccgagg acttcgacgg ctggcgcatt cccggctggg
     5221 atttcgcgtc gctcgcgccg tacttcgaga aggcggagag ccagctcaag gtttcagatc
     5281 tggagcgcca cgagctgtgc gacgccttca tcgcctccgc gatggcgctg ggcatcccgc
     5341 gcaacgacga cttcaacggc gcggcgcagg aaggcaccgg ctactaccgc gccaccgcct
     5401 accgcgggcg ccggcgcagc acggcgacgg cctatctccg gccggccgag aagcgtccga
     5461 acctgcgcgt ggaagtgggc gccctggcga cccggattct tttcgaggga aagcgtgcgg
     5521 tcggagtctc ctttcgccag ggtgaacgct cgcacatggt ccgggcagag aaggaagttc
     5581 tgctcgccgg cggggcattc aactcgccgc agctgctcga gctctccggc gtcgggccgc
     5641 gcgagctgct cgagaagcat ggcattgcag tggtgcacga cgcgccgaaa gtcggcgagc
     5701 gcctgcagga ccacttctac gtgcgcacgt tctggcgctc gcgcaggccg atcaccctga
     5761 acgacgacat ggcgagcctg tggcgcaagg caaggattgg tcttcaatac ttgttgaaga
     5821 aaaccgggcc gctgaccgtc agcgcgggcc atgccgcggc tttcgtgcgc accaagccct
     5881 ggctgaggcg ccccgacgcg cagatctatt tcatcaattt ttccaccgcg aagcgcggtg
     5941 gagtcctgca cgcgcactcc ggattcacct gcgccgtgtc gcagctccag gtcgagtcgc
     6001 gcggctcggt gcacatccgc tcggcggatc cggcggcgcc gccggcgatc cgctacaact
     6061 atctcgccgc cgagagcgac cgccgcgtga tggtcgaagg gctgaaggtg atccgccgga
     6121 tctgcgcgac gccgcccctg cgcgactacg tggccgccga atacctgccg ggcgagaaag
     6181 tcgtcacgga cgaagactgg ctggcgtact gccgcgagat gggcgaaacc gtgtttcatc
     6241 ccacgtccac ctgcagcatg ggcgccgtgg tcgacgagaa actgaacgtg aagggggtgc
     6301 agaaccttcg cgtgattgac gcttcggtaa tgcccgcggt gccgtcgggg aatatcaacg
     6361 cggcggtgat cgcggttgcg gagaaagcag ccgctctcat cacggcacaa tagcgcaatg
     6421 tgggaaggcg ccgcgattgt cctcattgcc gcagggatat ttctctggct cgacagcctg
     6481 cgcgcgcgcg agcgcgcggt gcgcgccggg cgcgcggcgt gcgagcgcta cgcgctccag
     6541 tttctcgacg acacggtctc gttcacccgc atccggctcg ggcgcgaccc ggacggccag
     6601 ctcaagatcg cgcgcaccta cactttcgag ttctccgata ccggcaataa ccgccgccac
     6661 ggcgcgatcg tgatgctcgg cggggagctg caggatctcc agctcgagcc gtatcgggtg
     6721 caataaggcg cggcaatgac ttccaaatgg ctcgaaagcc gggtgatcga gaaccggcgc
     6781 tggaccgatg cgttgttctc gctgcgggtc gagggaccgc agctcgggtt cgaggcgggg
     6841 cagttcgtcc ggatcgccgt ggacgagcgc atcgcgcggc ctttctcctt cgtcaacgcg
     6901 cccgacgacc cggtgctcga gttctacggc atcgtggtgc ccgagggtcc gctttcgccg
     6961 aagctgatgc aactccgcgc cggcgagcgc cttctcgtgg cgccgaaccc ggcgggattt
     7021 ctcgttttgt ccgaagtgcc ggatgccgac acgctatggc tcgtgtctac cgggaccggc
     7081 attgcgccgt ttctctccat attgaggaca gaaacgccgt ggcggcgctt ccgcaatgtc
     7141 gtgcttgtcc atgcagtccg tcacgctcgc gagctcgtct accaggacat gattcgtaag
     7201 attgtgaaag aaaagtcgct gcgctacctg acgttcgtga gccgcgaggc cgcttcgggg
     7261 tcgctgaccg gccgcattcc cgcggcgatg cgcgacggcc ggctcgagac cgcggcagga
     7321 ttggcgctga accaacattc tcacgtgatg ttgtgcggca accccgatat gctgaaggac
     7381 gcggccggcg cgctcgccga gcgtggcttg cgcaagcacc ggcgccgcac cccgggtcac
     7441 atcaccgtgg agagcttttg gtagctgttt tcagggggat ttttgtcctt ctcctggctg
     7501 cgctcacttg cgggtgcagc acggtgcgct tcgcttacga aaacgcggat gcgtacctgc
     7561 gctggaaggc cggcagctat ctcgacctgc agggcgaaga ggccgacgag ctcgacgacc
     7621 gcatcgacga atttcacgcc tggcatcgcc gacacgcgct gccgaaatac gtgggcctgg
     7681 cgcacgaggc atcgcagcgc ttctccgacg ggctatcgcg cccggacctc gtttggggtt
     7741 acgattcctt gagggtgcag gcgcgggaaa gcctgcgcaa ggcaggcgat ctcgtggcgc
     7801 cgctgctcga ccggctgacg cccgagcagg tggcgcagat cgagcgccgc ttcgccgagg
     7861 agaaccggca gttctatcgc gacaacctcc gcgcgccgga gcgtgaccgg cgcaagaagc
     7921 gagccaagca gaccgtcgag cggctggagg actgggtggg gaagctgacg aaagcgcaga
     7981 tccagcgggt gcgcgaatac gccgatcgga caccgatggt ggacgagctg cgcgaccgcg
     8041 accgcaagcg cgtgcagaag gacgtgatcg ccatcctgcg ggcgcgcgag gcgcgtgcgc
     8101 gactgggcga gcgcctcgcc cactgggacc gggggcgcga ggcggtgtac gtcgcctcgc
     8161 tggacctctg gcgcgaccag tacttcgcca tgctgatgga catcgaccgc tcgctcaccc
     8221 ccgagcagcg cgcgcgggcg cagggccacc tgcgccgcta cgcggaagag ttcgaggcgc
     8281 tcgcttcccg gtgaacgatc ccaagcccct gcgatcgttc gtcgagccgg ccgacgcgcg
     8341 cgtcgccgac cctggcgcct atgcggggcg cacgcgctac acccagctcg agtacgaggc
     8401 gctgctcgcc aatctgtcga tcggcatcgc gtttacccgt gagcggtgct tcttcctctg
     8461 caatccgaag ttcgccgaga tgttcggcta cgaatccagc gagctcattg gcatgcccgg
     8521 cgaggccgtc tacccgagcc gcgagagcta cctcgccctc ggccagatcg cggctccggt
     8581 cctctccgcg gggcgccagc tcgatctcga gtgggaggtg cggcgcaagg atggctcgac
     8641 cttcctgtgc cggctggtcg ccaagtcgat cgatcccgcc aatccgcagc acggcacggt
     8701 gtggatcgtc gaggacatca ccgaccggcg acgccacgcc gacgagatcg cgcgcctgct
     8761 gcgcgagcag gacgcgattc taggcaccgc gtcggtcggc atcgtgttcg tcagggaccg
     8821 ccgcctggtg cgctgcaacc gccgctacga ggagatgtac ggctatggcc cgggcgagat
     8881 ggatggccag ccggcgtgga tcatttatcc gggcacggtc gaattcgagg aggcgcagtc
     8941 ggtctacgag gcgctcgcgc gcggccagac ctcgcggcgc atcgggctgc gccggcgcaa
     9001 ggacggcagc acgttctgga accgcgccga cggccgggca gtcgatccgc agaacccgca
     9061 caagggctcg gtgtggaccg tcgaggacat caccgagcag cgccgcgcgg aggacgagct
     9121 gcagcgcgtg ctcgccgagc agcaggcgct gctcgacaac gtcgtggtcg gcattgccat
     9181 cagccgcgag cgcagggtga tgcgctgcaa ccgccgcttc gaggagatgt tcggcttcgg
     9241 ccccggcgag gcgaacgggg cctcgtggcg ccaggtgtac ttcaccgagg acgagttcga
     9301 cctgcgcgcc cagctctacg tcgagctcga ccaggggcgc acgcacgcga gggagcaatg
     9361 gctgcgccgc caggacggct ccggattctg gtgccgcata tcgggccgcg ccgttgcggc
     9421 gggcgacccg ggccgcgggt acgtgtggct gatggaggac gtgagcgagc ggcgccgcgc
     9481 cgacgagtcg ctcgagcgcc tggtgcgcga gcaggacgcg gtgctgcaga acgcggtcac
     9541 cggcatcatc ttcgtcaagg accgcaggat cgtgcgctcc aaccggcgct tcgaggagat
     9601 cttcggctac ggcgcgggcg agctcatcca gcgctcgacg cgcttcatgt tcgccacccg
     9661 cgaggagtac gaggccggtg gcgagacgct gtacgagccg gtgtggcgcg gcgagacggt
     9721 gttcatcgag cggcgccacg tgcgcaagga cggcgcgcag atctggtgct cgatctccgg
     9781 ccgcgccgtc cagcccggcg acccggcgca gggctcggtg tggctgttcg acgacattac
     9841 ccaggagcac gagtcggagg agcgcgtgca gcgcgcgctc gccgagcaag agctcattct
     9901 cgacaacgcc gcggtcggca tcgccttcgt gcgcaaccgc gcgatccagc gctgcaaccg
     9961 cttcctcgag gacatggtgg gcgccggccc cggggaactg atcggccaga gcaccgcgac
    10021 gctgttcgcc tcgcgcgagg agtgggagga cgccgggcgg cggacctaca cggccacgcc
    10081 gccgggcgaa accttcgaag gcgaagcgcg cttccgccgc cgcgacggct cgaccttcca
    10141 gtgccgcgcc cgcggacggc gcaccgacac cggcgaagcc gagcaggaat ggatctggag
    10201 ctacgaggac gtgaccgccg agcgcgaggc ggaaatgcgg gtgcagcgcg cgctcgcgga
    10261 gcaggacctg atcctcgaca acgccaccgt cggcatcgcg ttcgtgcgca gccgctcgat
    10321 cgcgcgctgc aaccgcttct tcgaggaaat gatgggcgcg aagcgcggcg cgctggtcgg
    10381 ggcgcgcaca tcggcgctgt tcgccgacga gaaggactgg gaggaggcgg cgcggcgcgc
    10441 gttcgaggag acgccgcccg gcggcatcca tcgatcggag gcgcgcctca agcgcgccga
    10501 cggatccagc ttcatctgcg ccatgcgcgg ccggcgcatc gactccgggg acgccgagca
    10561 ggtctggatc tggagctacg aggacgtcac cgccgagcgc gaagccgacc tgcgggtgca
    10621 gcaggccctg ggcgagctcg agcgcgcggt ggcggagcgg accgccgagc tcgaggaggc
    10681 gaaggcgcgc gcccagcacc tcgccgacca cgatgcgctc accggtctgc cgaaccgcag
    10741 gctgctcgag gaccgcctca cgcaggcgct tgcgctcggc catcgcaacc gcaagaacag
    10801 cgcggtgatg ttcgtcgacc tcgaccggtt caagatgatc aacgactcgc tcggccatgc
    10861 ggtgggcgac gcgctgctga aggaggtcgc cggccgcctg gtcacgcagc tgcgcgaagg
    10921 cgacaccatc tgccgcatcg gcggcgacga gttcgtggtc gtgcttcccg aggtgaagcg
    10981 ctcctcggat gtcgcccacg tggcgcaaaa agtgatcgag cagctctcgc agccggtcac
    11041 ggtggaggag cgcgagctcg tggtcacctg ctcgatcggc atcgcggtct accccgacga
    11101 cggccgcgac gccgagaccc tgatccgcaa cgccgacgcg gcgatgtacc acgccaagga
    11161 gcttggccgc gccaactacc agttcttcac cgagcagatg aaccaggcgg cgtcgcgccg
    11221 gctgcagctc gaaagcgacc tgcggcgggc ggtgggcaaa ggcgagctgc gcgtgcacta
    11281 ccagccgatc gtcgacgtgg cgagcggcca catcgccgcg cacgaggcgc tggtgcgctg
    11341 gcagcatccg gagcgcggcc tcgtcctccc ggcgcagttc atccagctcg cggaggagac
    11401 gggcatcatt ctcaagctcg gcgagtggat cctcggcgaa gcctgccgct ggaacacctt
    11461 catcggcgcc cgcagccatg acggcggcga gctgcagatc gcgatcaatc tgtcggcgcg
    11521 ccagttcaac gacccgcacc tgtccaaggt ggtggcgcgc gccctcaagg aaaccggcct
    11581 gccgccgcgg ctgctcgagc tcgagatcac cgagtcgacc gcgatgcagc agaccgacgt
    11641 cacgctgcgc acgctgaaga agctcaagca gctcggcgtg tcgatcgcga tcgacgattt
    11701 cggcaccggc tactccagcc tctcctattt gcggcgcttc cccgtggaca aggtgaagat
    11761 cgaccgcagc ttcgtcgccg aggtgccggc gaaccgcgac cagggcgcga tcgtttccgc
    11821 gatcatcgcg ctcgcgcacg cgctgcagat caaagtcgtc gcagagggcg tcgagaccga
    11881 ggcgcagcgc gagttcctga agtcctgcgg ctgcgacctc atccagggct acctcgtcgg
    11941 caagcccgcc gacgccgaca gcgcggccag agactacgtc taggcgcgcg cttcacttcg
    12001 cgcacgcgaa cggcttcccg gcctcctgct acggggcgtt cctggagccg ctgcgggaaa
    12061 aattcagagt ttcattcatc gacgcgatcg gcaccgatcc gcgctttccg ccgaccgaag
    12121 gctggccgca tctggtcgag cagttgatcg attccatccg agagcctgtg tacggcgtgg
    12181 ggcattccct gggcggctat ctgcactacc tggccgcggc gcgccgtccc gagctctatc
    12241 gcgccatcgt gctgctcgat gcgccgatca tcgggccgtt ccgcggcggc atgctcggtg
    12301 cgaccaagcg cctcggcatc gtcgaccggg tcacgcccgc cggcgccacg cgcgaccggc
    12361 gcagcacctg gcagacgcgc gaggaagcca gatcccactt tcggagccgc gcgctgtttc
    12421 gccgcttcac cgaggagagc atgaacgatt atgtgaacca cggcctggtc gagcacgggg
    12481 ggaagctgag gctgaagatc gacccgctga tcgaataccg gatctaccgc accatcccgc
    12541 acgacatgat gcgccacctg cgcagcctgc gcgtgccggc ggccttcatc ggcggcgccg
    12601 attcggacgt ggtgcggcgc gtgcggcttg cgggcatgcg cccgaaattc agcatgcgca
    12661 aggtgcccgg gggccacctc tttccgttcg agcacccgcg cgaagcggct acgtcgctcg
    12721 cccaggcgct ggaggatctt tcagcttctc ctcgatgagg cggatgttca tctccacgcc
    12781 gagcatgttg gggttgactt ccagcgcccg ccggtaccat ccgagcgcgc tgccccagtc
    12841 ctcgagctgg aaatagatct gacccattcc tgacagcgcg ccgaagtggc gcggattgcg
    12901 cttcagcacc tcgaggcagt cggcgatcga cttgtcatat tcgcccgcga ggtagtagac
    12961 cgtggcgcgg cggttccagc cttctgcaaa ttcgggtttt ctgtcgatca cgaccgaaaa
    13021 aagcccgatg gcgtcgccat gccttcccgc ctgcatcgct tcggcgccgc gcgccatcag
    13081 gcggtcgagc tccggatcgc ccgagcggct ccaaaggagc cacaggccct gctcggcaaa
    13141 ctggcgcacc agcgagctct cgtcgcgcag ccgctcgtgc agcagcggcg cgtcggccat
    13201 gctgccgtgg ttcgcgaccc acacgatcgc ctcggcgcgc gcggcggtat cggtattttt
    13261 taacgcggcc agcgcctctg tgcgggactt gggcggctcg gggaatgcgg ccggcgccac
    13321 gagtgttaac gcggcgagaa ggagtaccat cttcgctgaa tccgggagcc tcattggggc
    13381 gtatatccgg gggtcttcac caacagaggg aacacatgaa agcgcttttt cgtccgttca
    13441 tcttagccgg tctgctcgcg agcggcagcg tgctcgccgc gggcgatccc gtcaccacca
    13501 gcgccccgaa agacccggtc ctcgagaagg tctcggcggc caccgcccgc aaggactggg
    13561 caggcgccgc agctgtgctc agggatgccg tgtcggcgga cccgaacaac gccaactatc
    13621 acaatctgta cgcgtactcg atccgcaagg gcgtgaaccc ggacatgaac ctggtgttca
    13681 agcactacaa cgaggcgctg cgcatcgatc cgcagcaccg cggcgcgcac gagtacatcg
    13741 gcgaggccta cctgatggtg ggcaacgtcg gcaaggcgaa ggagcacctg acccagctcg
    13801 acaagctgtg cttcttcccc tgcgtcgagt acaccgacct gaagaaggcg atcgccgagc
    13861 acgagacgaa gcgctagcgc gctagcggtt cacccgcgcg aggcgccgcg cggcagcggt
    13921 taccgcggcg gccatcgccg ccgcggctgg cggcagcggc gcattggcgc gggcgaagag
    13981 ccccaccggc aagtccggcg cagtttccgc aatctcgatc tgctgcagga aaccgtgcgc
    14041 gtagggctgg gcgaggagct gaggcacgac cagacccagc gtatcggtcc gggcgagaag
    14101 cgcgagcgcg gccgcatacg attcgcaatg cacgacgact ttcggccccg gcagtccggc
    14161 cgccgagaaa aaccgctcca gaatgccgcc ctgtccgcgc gggctataca tcagccagga
    14221 agcatcggcg agctcgcgca gcgagtgcgc agcacgcaga ggatggccac gccgtccggc
    14281 aaccacgagc cgcgcgcggc acagcggccg gtgccggatc gcggggtcca gcgctccggg
    14341 cggcttctgc ccgacggcga aatccagcgt ctcgtcgcgc agcagaggca acagtccctg
    14401 cagcatgccc tcgactacgc gcaccgctgc gaagggatat ttctggcgga agcgcacgtg
    14461 cgcttcgggc acgaacagca ccgcggcgga gggcgagacg ccgaaggcga cggcgcctgc
    14521 gcgctcgccc ttcaagtcgg ccagatcttc cgtcgcgcgt cgtagctcgg cctgcacgac
    14581 gcgcgcccga gtgaggaacg cgtgcccgga gcgcgtcggc gatacgccgc ggctcgaacg
    14641 ctgcaagagc tgaacgccga gctcgttctc aagctgacgc aggctcttgg tcagcgccgg
    14701 ctgggatacg ccgacgcttc tggctgcagc gcgcaggctg cccgcttcga ccgtgacgat
    14761 gaaatctcgg atctgattca ggcgcatggg tgatagctcg cggttatcgc aggagtgtcg
    14821 gcggcatctt acgctccagc gcgcggcgtg atttacttgc gggatgcgca ctcttctagc
    14881 gatcctcgcg ctggccctgg cccaggcggc ggcggcccag gctttccctt cgaaggcggt
    14941 gcgcctcgtc aacccgtttc cgccgggcgg gccgctcgac gtgctcggcc ggattctgtc
    15001 cgagcgcctc ggcgcttcgc tcggccgccc ggtgctggtc gagaacaagc ccggcgcggc
    15061 gggcaacatc ggcgcggccg aagtggcgaa ggcgccggcc gacggccata cggtgctgct
    15121 gacgctgttc agcatcatca ccagcaatcc gcacctctac gaccggctgc cgttcgaccc
    15181 gcttaaggac ttcgcgccgg tcacggcgct cgcaagctcc gattcggtgc tcatcgtcca
    15241 tccggctctc cccgcggctt cggtgaagga gctcatcgca cacgccaagg ccaatcccgg
    15301 caagctgaac tacgcctcgg ccggcaaggc ttcccctgga catctcaccg ccgagttctt
    15361 cgcccggcgg agcggcgcgg cgtttactca cgtaccgtac aagggcaacg ccgaagcgat
    15421 ccgcagcgtg cttgccggcg agacccagat gatgttcacg ccgacgacgg gcgctttgcc
    15481 gcttatcaag gcgggaaagc tcaaggcgat cgcggtctac ctgaccgagc gcgtcgacga
    15541 gctgccggag gtaccgagcc ttgaagggtt cgatcagaaa accctgccgt tctggtacgg
    15601 cctcttggcg cccgcgggca cgccgcgcga gatcgtgatg cgcctcaacg cggagacggt
    15661 gaaggcgctg aaggatcccc aggttaacaa gacgctgcgc gccggacgca tctttcccat
    15721 cggcaacacg cccgaggaat tcggcgccat gatgcgcgag ggccatgcgg cctgggggcg
    15781 agtgatccgc gagaccggtg tcaaaggcga gtaggccgag cttcgtcttc atcctcgccg
    15841 acgacctcgg ctatgccgac ctcggctgtt atgggggtcg gggttcgtgt tctccaaatc
    15901 tcgatcgcgt ggcggccgag ggattacggc tcaccgacgg ctacgccaat tcgccggtgt
    15961 gctcgccgac gcgcttcgcg ctggcgaccg gccgctggca ataccgcctg cgcggcggcg
    16021 ccgacgagcc gatcgcgagc cgcaaccgcg gcagcgccgt cctgggactg cctcccgcgc
    16081 accccacgct cgcctcgctg ctgcgcgatg cgggttacgc caccgcgctt gtcggcaagt
    16141 ggcacctcgg ctttccgccg cacttcagcc cgctgaagag cggctaccag gagttcttcg
    16201 gcccgatgag cggcagcgtc gactacttca cgcattgcga ctcggccggc gtgcatgacc
    16261 tgtatcaggg cgagaaggag gtccaccgca ccggctatct cactgacctg ctgagtgacg
    16321 aggcggtctc cttcgtgaag aaagcgaagg gcaagccgtt cttcctctcg ctgcactaca
    16381 ccgcaccgca ttggccatgg gaggggcgcg ccgaccggga tgaatcgaag cgcatccgca
    16441 ccatcagcca tttcgacggc ggctcggtga agacctatct cgaaatgatt cgcgccatgg
    16501 acgaaggcat cggccgggtg atgcaggagc tgccgggaaa cacctgcgtc gcgtttacca
    16561 gcgacaacgg cggcgagcgc ttctccgatt cgtggccgct cgtcggcggc aagatggatc
    16621 tgctcgaggg cgggatccgc gtgccttatg tcgtgcgctg gccgaaaggc gccatcctgg
    16681 cgcagcttgc gatcaccatg gactgggtcg ccaccttcct cgacgccgcc gatgtcgcgg
    16741 cgcacccgga ctacccgctc gacggggtga gcctgctgaa ggagagccgc gggcgcgagc
    16801 tcttctggcg catgaagttc cgcgaccaga aggcgatgcg cagcgggaag tggaagtggc
    16861 tctcgatcga aggcaacgag ttcctgttcg acctctcgaa cgacgcgcgc gagcgcgcga
    16921 acatggcaaa gcgccaaccc gcgaccttcg aggaactgag gaagcgctat gcggtttggg
    16981 atgcttcgct gcctccgatt ccggacgacg cgaaggtgtc gctcatcggc ggaccgcagg
    17041 agatggcgaa gccgtcctag cgtgctagct tggcgcgcag tttcccgtaa tagcggctct
    17101 cgatccgcac ctgcatcagc ccgagcttct ttacctcggg cggcgtgacc ccggcgttct
    17161 tgaggatccg gttggcgacg cgcagctcct cggggatcag cgcatcgtcg tcgagctcga
    17221 gcggccggcc ggcgccgggc aaatcgtcga actcgccgca tgccatagct tcctgaatct
    17281 tcctttcgac gaggaagtcg agcatgctca gtatcccacc gcagcaccgt cgctgcgagg
    17341 atccgcgccg ccggcgagga tgccggtctc gccatcgatc gtgatgccgt tggcatggcc
    17401 ggcgagctcg ttccactcgc cccagcgatc gacgggatgg ccgcggcgca cgagctcgtc
    17461 gatggttgcg ctcggatagc gcgcctcgat gtgcagggtg tcgcgcgctt cgccaagccc
    17521 gaagcggccg gagagccagc gaggcgcgct gagcgcctgc tggatgtcca ggccgaagtc
    17581 gatcatcgcg acgtaggttt ggacgtggat ctgcggctgc ccatcggcgc ccatgcagcc
    17641 gagcacggcc cacggctttt cattcctgaa ggcaagcgag gcgatgagcg tatgcagcgg
    17701 taccttgccc ggctcgagac ggttggggtg cgccgggtcg agcgagaagt acgcgccgcg
    17761 gttctgcagc accacgccgg tcgcggccga agtcacgcag gcgccgaaaa cgccatacag
    17821 gctgaagatc agcgacgcgg cattgccggc agcgtccacg gccgcgacgt acaccgtgtc
    17881 gccggtgagg ctgccctccg agggcacctt gtcccaaggc agcgcgcgcg ccgggtcgat
    17941 cagccgccgg cgctggtccg catagggttt tgacaacaat ttttcaatgg gtacctcgac
    18001 gaagcgcgga tcggcaagcc agcggtcgcg gtcgttgtag gcgatctgct tcgcttgcac
    18061 cagcaggtgg acatggtccg ggcccaggaa ctcgcgcttc gagagctcca gcggctcgag
    18121 caggttcagc atctcgagca cggtgaagcc ctgcgtcggc gccggcgttt cgtagatcgt
    18181 gacgccgcgg tagctgccct ggatcggctc tccccagtac gcgccctgcg cggcgaggtc
    18241 gcgctcggtg aagaaaccgc cgagcttcgc gagctcccgc gccacctcgc cctcgtagaa
    18301 accggcgcgc ccgcgcgcgg cgatggtgcg caacgtgtct gccaacttcg ggttcttcaa
    18361 gaccggaccg ccgcgcagga aaatcgctgc ggtggcggcg tccatctccg gcgcggtctg
    18421 ctcgatccag cgcgaaacgc gcgcactgac cgggaaaccg tcggccgcat agcggatcgc
    18481 gctctccagg cagcgcgcga acggcaggcg gccatggcgt gcgtgcgcct cgcagaagct
    18541 cgctaccgcg cccggggtag tgagcgtggc gggtgcgaga ccgcggaacg ggatctcggc
    18601 ctgcccggcg aagcgctcga gcatcgccgc cgccgccgct ctaccgccgc cgtcgaggta
    18661 ggaaactttt cttgccttgg cgtcgtagat cagccagaac gcgtcgccgc cgatgctgca
    18721 catgtgggga tacaacacgg cgagcgaaga cgcggcggcc accgccgcat cgaccgccga
    18781 gccgccgctg cgcaaggcgt cgacgccggc cgcggaggcg agcgcgtgcg ggcacgccac
    18841 catgccgtgc gtcgcgaagc tgggagccat gctcgagctt actgcttgcg gctcctcgcg
    18901 agcgtgactg aagggctctc gccgaagcgg ctgcggtact cgatcgcgaa gcgcccggga
    18961 tgggcgaagc cccagtgcag ggcgcacgcg gcgacttggg tcgcagttcc cgaagcgaat
    19021 tcgtcgcgca cgcgctgcaa ccggaggttg cgcagatagc gcatcggcga cacgccgtgg
    19081 aagttgcgga aatgctgcag cagggtacgt ccagggacac cggacaccgc gaccaggtcg
    19141 gcgagcgtga gaggctgcgc gaggttcgcg tggatgaagt ccagcgtgcg gcgcacgtcg
    19201 cgcggcgcga cgcggcgctc gacgcggcgc agcgcttcgc tgtagttgtg cggctggaaa
    19261 agcagcaacg cgtcgagcac gaacgcttcg aagcgcgccg cgaccagcgg ctcgccgagg
    19321 gacgcgccgc gctcggactc ggcggcggcc cagtgcagga tcccggcgag ccggcgcccg
    19381 tgcccggagg tcgcgtcgat cgacggcgca aactccaccg cctggcgcgg cgcctcgcct
    19441 agcatcgcgg cgagatgccg gttgagcgcc tcctcgcgca ccgcgagcga aagccgcgca
    19501 ttgccgttcg aggtgtgcag cgtgttgtcg ctggtgggcg agagcacgat gccgcgatcc
    19561 tcgtccccgt cgcaggtttg accgccgacg agcgcctgca tgtcgccctg cagtgagaaa
    19621 tgcacccagt acaccgccgg cggctgcctg cgctccgact cgcgggcaag ccacgggctc
    19681 gagcggcgcg agagccgcag gcgcaaggcc gcgccgtact tgaggtacgc cacccactga
    19741 cccggcagga aaagagcgtt cagccgtacg tccatgggga attcctgtcc ccgcgggtcg
    19801 gcgagggcca tgtcgatcgc gtggctgcgc gcgagataag cgcgcgtttc ctcgaagctg
    19861 cgcgaacgca gcatcggtgc gtggtcgagc aggggctgca tggcgggaaa agtagcaccc
    19921 gccgcgtttc cgcagtgttt ctgcgccgtt tccatttgga aacagcgatc cgctacttct
    19981 tgcgcttggc cttcgcggaa accgtcctct gtccctgttt gccgagtttc ttgcgcgcca
    20041 aggcggcgcc cgcggtcagg gcctggagct tccttagcgc aatctcgcga tccatcggcg
    20101 ccatgccgca gttggtgcaa ggaaagagat ggcggacggg cacgaacttg ctggccctct
    20161 ggatgacctg ggaaacctct tccggcgttt cgaccttgtc gctcgcgacg tcgatgacgc
    20221 cgacgagcac gtccttgccc ttgaggagct tgagcagctc gaccgggact ttcgagtgga
    20281 tgcactcgag cgagacctgg tcgaccttgc tcttcgccag cgcggggaag atctcttcgt
    20341 actggcgcca ggactcgccg agcgttttct tccagtcgag gttggcctgg attccgtagc
    20401 cgtagcagat gtggacgcaa gtggtgcact tcaggcccgc gatcgcgcgg tgcagggcat
    20461 cgatgcccca gcgcttgacg tcgtccatgt agacgttgaa cgccggctcg tcgatctgaa
    20521 tcacgtcgac gccgtctttt tccagcgcgc gcgcctcctc gttgaggagg ccggcgaagg
    20581 ccatggcgag cttcaccttg tcgccgtagt ggcggtccgc gatcgtatcc acgatcgtca
    20641 tcggcccggg catcgtgatc ttgatcttct tcttcgaatg cgctcgcagg agccgcgctt
    20701 cggcctggtg cacgcggccc ttgaggcgca gcggcgccac gacgagcggc accatggcgt
    20761 cgtagcggtt gttgcggatg cccatcttca ccttctgctc gaagtcgatc ccctcgacga
    20821 actcgaggaa gccgtggacg aagtgctgcc gcgattgctc gccgtcggtg acgatgtcca
    20881 agccggcgtc ctcctgctcc ttcagccaga gcagcgtggc gtcgcgcttg gcgcgctcga
    20941 ggtcggttcc ctgcgtccgc catgccggcc aaagcttgcc ggtctcggcg agccaggcgg
    21001 gcttgggaag acttccggca atcgtggtct cgaacatgtg tcgggctccg gggacagcga
    21061 cgaaatcgtt acgccttaat ataatgtaca gctacaaggg aggattctgc ggtgttcggg
    21121 atggtggtgg gaacgttctg ctcgttcgtc atgggactct tcaatcctta catcgcgaac
    21181 aaggccggtc ccgactggat gtggggcggc ggcaggaacg atttgctgcg caacctctac
    21241 ttccgccacg acgggtcgtt ccggcgctat ggccgcatcg ccctccttgc cacgctgttc
    21301 gggggctcgg ccgctctctt ttgggtgctg tagagatcgt ccactccggc ggctgccact
    21361 gcggccgcgt gcgcttcgaa gtgaccgcgc cggcggcgat ttcggtggcc gagtgcaact
    21421 gctccatctg cgccaagtcc gcctacctgc acctgattgt gccgaaatcc cgcttccgcc
    21481 tgctccaagg cgaggaatcc gtcacgactt acgccttcaa caccggcgtc gccaagcact
    21541 tcttttgcaa agtctgcgga atcaagtcat tctatgttcc gcggtccgac ccggacgggg
    21601 tcagcgtgaa cgcgcgctgc ctcgaccccg ggaccgtgac gcgcatggaa gtgcggccat
    21661 tcgacggccg gaactgggaa gcatcgatag gggagaagtc atggtaaagc tgatcgctgc
    21721 ggcagtggtg gtgggcttgg ctgtctcggg gccggctttg gctcagcgca aggattgcgg
    21781 agagctgaag ggcgagatcg aggccaagat caagaagaac ggcgtcgaga agttttcgct
    21841 cgacgtggtc gacgccgaca agcaggccga aggcaaggtg gtcggcacct gcgacggcgg
    21901 cacgaagaag atcgtctaca agcgcgacta gcgcttcgcc ccgccggggg cactgagcag
    21961 ccaggccgcg gccgcggcct gcgccgccag cagtaacccg aaggccacgc gataggcttc
    22021 cgggtcgtag cggccctgcg cgctcggcca gtggccgagg attgcgccga ctccccattg
    22081 gaatgcgaac gaggtcccga acagcagcag gttcgacgcg gtggtgacgc gcccggtctg
    22141 cgccgccggc acgagctgag tgagcagcga gtaggcgatg atcgacgcga tgctggttgc
    22201 ggcgaacagg accaggaaga gcagcgctcc gcgcgaggcg cccaaagcga gcggagcgaa
    22261 agcagcagcg cagatcagca tccccgcctg atagaagcga agcggcgcca tgccgcgctt
    22321 cgcggccgca tcggaaaccg gcccgagccc gagcgaagcg gccatatagg ctgccgcgga
    22381 aaaaaagagg taatcggcag cggccgcgcg gtcgagcccg tgcacgtcgg cgagccacgg
    22441 cgcgaaccac agcccctgaa gcgcctggaa cgtcgcctgg caaatcacca gcggcaccgc
    22501 gaggcgccag aagtcaatgc gcgcgaagat cctgccgatc acgcctacct gctcgcgcca
    22561 tgggtcgcct tgccccggaa gcgcgcgctc cggcacgacg aagaagatga gggccgccgc
    22621 cgccacgctt agggcggcca gcgcaacgaa cagggcgcgc cagccgaacg gtccgaggag
    22681 cgcctccacc ggcgcggtgg ccgacagcgt ccccaggctg ccgatgccga tcatccagcc
    22741 gctcagcgat gtcaggcgct cgcgcggaaa ccacagcgtg aacgccttga tcgaccccat
    22801 caggcccgcc gaaacgccca gtccgatgag cgcgcgcccc atcgacagca tcgccaggtc
    22861 gcgcgccagg ccgaagacca gcgcgcccgc gccggcgacg cagaagagcg cagccgtgca
    22921 gcggcgcgga ccgaagcggt cgagcgccac gccgagcggc agctgcgcga gcgcgaacgc
    22981 gaggaagtag gcgctcgtca gcaggccgag gtccgagggc gcgataccga agtcggcgac
    23041 caggtccttc gcgatcaccg cgttgacgta gcggtagaag tacgagaggt agtagcccgc
    23101 cgcaaagggg gtgaacacca caagcatcgc gttcaaaggt aggctagccg tcatgaagaa
    23161 gcggctgatc gtcggaatta cgggagccac cggggccgtc tacggtgtcc agatcctgaa
    23221 aaccctgcga gacaccgacg gctgggaaag ccatgtgatc ttgtccgacg ccggagcgct
    23281 gaacctctgg cacgagctca agatgaagcg caaggagctc gagaagctgg cggacgtcgc
    23341 ctaccacccg aaggacatcg cggcgacgat ctcgagcggc tcgtttctca ccgaaggcat
    23401 ggtgatcgcg ccctgctcga tgaagacgct cgccgcggtg gcgcacgcgc acgccgacga
    23461 tctcctctcc cgcgccgccg acgtggtgct gaaggagcgc cgccgcctgg tgctggtgcc
    23521 gcgcgagacg ccgctcaacc tcgcgcacct gcgcaacatg gtggcggtca ccgagatggg
    23581 cgggatcgtg tttccgccgg ttcccgcgtt ctacgcgctt cccaagtcgc tcgaggatct
    23641 cgtcgcgcac acctgcgcgc gcgtgctcga cctcttcggc gtgcactccg ctaagctcgc
    23701 gcgctggcag gggatgaaag gaactcccga agagcgcggt tgaaagcctc ggcctgctcg
    23761 atgttggcga tgtgggccgc ctgcgggatc atcaccagct ttgcccccgg cacgttctcc
    23821 ccgatgtagc gcgttcccgg cgcggcggcg tcctgctcgc cggcgatcgc cagcaccggc
    23881 cgcttgatct ccttcagtct cgcggtggtg ttgaggttcg agatggcgtg gcagcagccg
    23941 acgtagcccg gcaccggggt gctttgaatc aggtccgcga tttttttcac gatctcgggc
    24001 ttcccctttc tgaacggctc ggtgaaccag cgttcgagcg tcccctggac gagcggctgc
    24061 atgcccttgg tctcggcggt tttaatgcgt tcgtcccact gcttgcgcgt ttcgggcgtt
    24121 tgcgtgtgcc cggtgtccgc gaggacgacg cggtcgaaca cgcccgggtc ggtgagcgcg
    24181 accacctggc cgatcatccc gcccatcgac aggcccacgt agtgggtgcg ttttattttt
    24241 aactcggcca gaagttggcg cagatcttca gccagcatgt cgagcgaata aggacccttc
    24301 ggcgcttcgc tgccgccgtg gccgcgcgtg tcgtagacga ggatgcgaaa gcggtccttg
    24361 aactcggcga cctgcccgtc ccacatgcgc aggctgcagg cgagcgaatg gctgaaggtg
    24421 agccaaggcg cgccttcgct tccgtggatc tcgtagttgg cctcgatgcc gttcactttg
    24481 gctttcatgt cgcctccagg atcaatggga tcagcatctc gtcgtcgctc gtgccgccgt
    24541 ggttgccgat gtgcaggtgg cgcgactcgc ccgtcgtcca gtccttgacg gtgtagcggc
    24601 cgcgcatcac gagcgtcacg tcgccgatgc gctcggcgaa gcgcgggtgc ggcgtgcccg
    24661 gcccgaacca gccttcgtct acgagctccc tgctcggcat tacgtccgcc cgctcgccga
    24721 gccattcttt tgctttttcg gtgaaggttc ttgggtcatg gacatggcaa tacgccacgc
    24781 gccgctcgcc gcagagcggc agcctcaaca acggagccag agactgcggc aactcgaacg
    24841 attcctccgg cgcgacgtcg atgaagccgt ggtccgccgt cgcgatcacc agcgagtccg
    24901 ttccggcgag gcgcgcgagc agcttgccga aggcctggtc gatttttccg agctgctcga
    24961 aagcctcggc gctttccgag ccgtagcggt gcgaaaccat gtcgtacacc ggccagtaca
    25021 cgtagacgaa cttgcgaccg gcccccgact tgaccgcggc ctcggtctgc gctacgagct
    25081 cgtcgagcgt ttcgtaggca aggcgctgcg cgccgcggca gtggcacatg ttgtattccg
    25141 agtcgacgat ctctttgtag gtcacgacga aagaagcgtc ggccagggac tcgaagatgg
    25201 agccgcagac gtaaacctgt tccggtttta ccccgcgccg tgcgagcggg agatagtccc
    25261 cgcggctcct gaaaggcagc gccgcagcta cgcagcccgc ctcgccgaaa taggtgaacc
    25321 agccggtaag gccatgctcg agcggcgtgc ggccggtata cgacgtcgtg atggccgaag
    25381 ctgtcgtcga ggggaacacc gaggtcagcg atgcgcggcg ccgccgcccc agctcgccgc
    25441 ccgcgccgcg gcgcatgagg tagttgtcgc cgagcccgtc tatgatcatt aatacgacgt
    25501 tgcgcgcatc gcgcagcttg tcttgcggca gatctctgag cggaagatgc agcgccttgc
    25561 cgccgcgcgc cgcgacgatc ga
//
LOCUS       DATBSZ010000036         8560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_824363, whole genome shotgun sequence.
ACCESSION   DATBSZ010000036 DATBSZ010000000
VERSION     DATBSZ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824363"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            630..1979
                     /locus_tag="VIG70_02530"
     CDS             630..1979
                     /locus_tag="VIG70_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249453.1"
                     /translation="MRRKLLGWGAALIAVAAGFSIWQDRMGVQANRSEHPKYVVDASW
                     PRPLPHQWLIGQVPGIAVDKHDNVWIVQRPRTLTTDERAASNFEPGTPPALDGAARPQ
                     GALADCCLPAPSVMQFDGKGRLLRAWGGPADPEFGVGGPGSKCEEPNCQWPNTEHGIY
                     VDHKMNVWLGGQAGGAVLKFTQDGDFLLQIGRRQPPVRNSNDTNGGINGTPLLAQPAD
                     MEVDPRTNELYIADGYTNLRVIVVDAETGMYKRHWGAYGQNPVDDTLDEPGSGGAYIK
                     NQAPSPNFRNPVHAVRITNDGLVYVADRVNNRIQVFDKRRVGAVCQNPMRAPGVCGFV
                     GEIFVERDTLSNGSAWDVDVSPDRDQKWLFNADGTNQYIWTLLRKTGQIVDRFGRNGR
                     SAGQFHWVHNLAVDSKGNIYTAEVDTGKRAQKFVQLDDNDHNRGRGRGEDDDDDGRR"
     gene            1991..3328
                     /locus_tag="VIG70_02535"
     CDS             1991..3328
                     /locus_tag="VIG70_02535"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEY8249454.1"
                     /translation="MHRSRPAPKGAGRLFACLLAAVLSLPAAAHDIPDEIVLHAFVKP
                     EGDRLHFLVRVPLAMLLNLDLPKRGPGFIDLPLPEERLQAAAAATAKELVLQENGTAM
                     TPRRAAARISEASDKSFETYARALARIAGPKLPDATNVFWNQGYFDAHLEYPIGSERS
                     DFSLDMRLAPGLRGRLKLILRYLPPDGSTRAYELHGGAGHVTLDPRWHQAAATFVASG
                     FRHILGGVDHLLFLLCLVLPFRRIGWTLVAVITSFTVAHSITLIAAARGMVPPGTWFA
                     PLVEVLIAASILYMALENIVRPNLRWRWAVTGLFGLVHGFGFSFLLQSQMQLAGEHLL
                     LSLLAFNVGIELGQLVFIGVAIPLLFLLTEKLRFDARLTSVVISAFVAHAAWHWMTER
                     WDELAKQPWPEVDPWQLALWCLLAAALAVLAWALLGRVSVLRRREAEPGVKPH"
     gene            complement(3269..4096)
                     /locus_tag="VIG70_02540"
     CDS             complement(3269..4096)
                     /locus_tag="VIG70_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8249455.1"
                     /translation="MSGIPARYAAATGAQPVFLLEGTGGLAAAKWRVPPHRVPNLWAP
                     HHLLGYRVSGMSTIVRTCRGVVQRKVPPIGSVTFSPGDCPVEWSSDAPIEAIHVYIPP
                     EALRGIRDFFGITDPWLAAYCRLLDSERALYDDSLFLQQTQHFLLRHLVRHYSNVSKR
                     TPEPRLRVTPLGRGVIRRIEDYLEANQRSDVPLQALAALAHLSVDHFVRAFRGATGKT
                     PHRFALERRLDRAAAMLKGPATIAHIARACGFRSPAHFSVRFHARFGLTPSQYRHSA"
     gene            complement(4098..5558)
                     /locus_tag="VIG70_02545"
     CDS             complement(4098..5558)
                     /locus_tag="VIG70_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8249456.1"
                     /translation="MNCKTTMAVILAGALGAFSAAAAAHDRDDDDRDDRGGWKPVDPA
                     LVKARQKFFGVENVDSRGRVNEDKVIFSWSTNTTYVVSVKGRVLMLDSYLLHVELPTD
                     GPIDRRRTKALPKDFVDLKPEAIFLGHGHGDHADNAAYVAKWTGATIYASPETCDVMQ
                     QDVTRMWNDPNEHNGGAKIIPNGDPVDCVGAVPRNSPPGEYSGTLENPTGGTTRVRRI
                     KQFDRDICILAFKHIHSGNAPVDPSFAHSTLNDLADPRDAGRVFTTPAVTYPALYPTG
                     IPYTPPGNPASRVPGQLNTTTTGFGGAAGIIEIGYHFVLRGDNNFAFSYINSAGPVKE
                     GIGSGSPGLISIAQYNDPVNNGPAIALAAEIGKGLFSLMEDLPQTDVMLGSVISLGAN
                     NNQQRDIISYIQRLKPRVFVPGHFSAVAQRGSSPYYLVNWRETALAMGFPESQWPELR
                     WMVDPLDYFRPMVYDPNDRRWAKSHHAEHKYREHCR"
     gene            complement(5627..7894)
                     /locus_tag="VIG70_02550"
     CDS             complement(5627..7894)
                     /locus_tag="VIG70_02550"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HEY8249457.1"
                     /translation="MSKTNLRLSACALLCAVAVSPVYAHDDDDDDGDRRHRGSSAFRI
                     EVLSSKPHLVSGGDALVRVTVKKKDVRLSDVRVELNGRNVTGALVANHGAKTLTGVVG
                     GMRLGDNRLEADSKRHGVAEAELKLTNYPVTGPIISGPHQFPFACTTQDFVPYPGGAA
                     LGAPLDANCSVARRVNYVYRKTNNTFALLPQPYSTPPADLAMTTTVTGATVPFIVRLE
                     TGTINRAIYQTAILHNPNSGEPSPAAPPAAWNGRVIYPLGGGCIAGWYTQGDGLVTPL
                     NATWLGKGHAVASASLNTFGNNCNDLLSSETILMVKERFIESYGVPKFTIGTGSSGGA
                     YQSNQTGDNYPGTFDGIVTMNSFPDVTTGMIPMHSSRLFELYLASRPGRYTVEQVKAM
                     TGYLSINPDLFVTGQIHEMSRERGDRMDPRVAFPDAILPGVGPQFRYDPVTNPYGARG
                     DVYDHTVNVYGVIRNTPFPGDSKFAQRPLDNVGVQYGLGAMNDGVISVEDFLDLNANM
                     GGVDIDFNRVPNRTVHYPDATKRAYQGGRILSGGGGLASTAIITRSGGNDLLVAGDVH
                     LRYHSFSIRQRIINANGHANNQVIVGNLAPSELLIDQMGQWLTAVVADTSNRSRAAKV
                     VANKPADVVDACWTTAGVKIVEPQVLYGPGQCNVLFPRGVPPEYVAGAPIELDAIKCH
                     LKRIDMSDYKVAFTSQQRARLSQIFPKGVCDWSKKGVKQVKVVPFASFGPSPENLVFD
                     VTDRRRHDDDDDDDD"
     gene            complement(8068..>8560)
                     /locus_tag="VIG70_02555"
     CDS             complement(8068..>8560)
                     /locus_tag="VIG70_02555"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEY8249458.1"
                     /translation="VVKRAAGVRDDTDLSDFRRRGGKIIFYHGLSDSGPPWTYTAYFY
                     DRITDRDHGRHSRHRDFARLYMVPNMGHCSGGPSTDRFDFLTPLMAWVEERKAPDAVV
                     ATGVSFKTAPTTRSRPLCPYPQTLRYTGPAGGDIALAANYTCREWPTRGRGDRDDDDD
                     DDD"
BASE COUNT         1303 a         2806 c         2999 g         1452 t
ORIGIN      
        1 cgcgtgacga atcgcacgat ccagccggga acaccgcctg cagccgtgcc cactgcgcgg
       61 ggggaacgcc gccgcgtaat ctcgctgcct tggtggcttg cgctggcact tgatgatgtc
      121 cgcggcgccg tccgcgcccg ccacctcccg ggggaaggag ttggcgaggc aggctaggcc
      181 gcaggcggtg ttttccgctt acgtccgaag ctcaatcgtc atcgtcatca tcgtcgtcat
      241 cgtcgtggtg accgtgcttg cctttcggct tgcacaggtt cgcggcgatg gcgttcagct
      301 tccggtcggt gctggtcgcg ggcctcggag ctcgcggctg cggcgcacgc aagcaatgcc
      361 gccgcaacgc gggtggcgcg aaaactcatg gtttcttccc cctagtgtgc caactgacaa
      421 ggccaaagct acttggtcgc agcgcaagca tccatgaaac ttgtttctga cctgattgcc
      481 tcggctcatg aatcgccatc ccaaagaaat aaagtcgatt tccggaaaga ttgtcgagaa
      541 cgcgaaagga attgatcccg ccgtctttga cccaaaggcg agcgcggccg aacatcggcg
      601 acgttgtttt tatttctgga ggagacgcca tgagacgcaa gctgctcggc tggggcgcgg
      661 cgctgatcgc ggtcgcggcc ggtttcagca tctggcagga ccggatgggg gtacaggcca
      721 accgcagcga acatccgaaa tacgtggtcg atgcgtcctg gccgaggccg ctgccgcacc
      781 aatggctgat cgggcaggtg cccggcatcg cggtggacaa gcatgacaac gtctggatcg
      841 tgcagcgtcc ccgcacgctg acgaccgatg agcgcgccgc gtcgaatttc gagcccggca
      901 cgcccccggc gctggacggc gccgcgcggc cgcaaggcgc gctcgccgac tgctgcctgc
      961 ccgcaccgtc cgtgatgcag ttcgacggca aaggccggct gctgcgcgcg tggggcggcc
     1021 cggcggatcc cgagttcggc gtgggcggtc ccggcagcaa gtgcgaggag ccgaactgcc
     1081 agtggccgaa taccgagcac ggcatctacg tcgaccacaa gatgaacgtc tggctcggcg
     1141 ggcaggcggg cggcgcggtg ctgaagttca cccaggacgg cgatttcctg ctgcagatcg
     1201 gccggcgcca gcccccggtg cgcaacagca acgacaccaa cggcggcatc aacggcactc
     1261 cgcttctggc gcagccggcc gacatggagg tggacccgcg gacgaacgag ctttacatcg
     1321 ccgacggtta cacgaacctg cgcgtcatcg tggttgacgc cgagaccggg atgtacaagc
     1381 gccactgggg tgcgtacggc cagaacccgg tcgacgacac cctggacgag cccggctccg
     1441 gcggtgccta catcaagaac caggcgccgt cgccgaactt ccgcaacccg gtgcacgcgg
     1501 tgcgcattac caatgacggg ctggtctacg ttgccgatcg cgtcaacaac cgcatccagg
     1561 tgttcgacaa gaggagagtc ggagcggtgt gccagaaccc gatgcgcgca cccggcgtgt
     1621 gcggcttcgt cggcgagatc ttcgtcgagc gcgacaccct gagcaacggc tccgcttggg
     1681 acgtcgacgt ctcgccggac cgggaccaga agtggctgtt caacgccgac ggcaccaacc
     1741 agtacatctg gacgctgctg cgcaagaccg ggcagatcgt cgaccggttc ggccgcaacg
     1801 gccgcagcgc gggccagttc cactgggtcc acaacctggc ggtcgactcg aagggcaaca
     1861 tctacaccgc cgaggtcgac accggcaagc gggcgcagaa gttcgtgcag ctcgatgaca
     1921 acgaccacaa ccgcggccgc ggccgcggcg aggatgacga cgacgacggc cgccgctaga
     1981 ctcctcgtcc atgcatagaa gccggccggc gcccaagggc gccggccgtt tgtttgcgtg
     2041 cctgctcgcc gcggtgctct cgctgccggc ggcggcgcac gacatcccgg acgagatcgt
     2101 cctgcacgcc ttcgtcaagc ccgagggcga ccggctgcac ttcctggtgc gggtgccgct
     2161 ggccatgctg ctgaacctgg accttcccaa gcgcggcccc ggcttcatcg acctgccgtt
     2221 gcccgaggaa aggctccagg ccgcggcggc cgccacggca aaggagctcg tgctgcagga
     2281 gaacgggacc gcaatgacgc cccggcgcgc ggcggcgcgc atctcggagg cgtccgacaa
     2341 atccttcgag acgtacgcaa gagcgctcgc ccgcatcgcc ggaccgaagc tgcccgacgc
     2401 gaccaacgtg ttctggaacc agggctactt cgacgcccac ctcgagtacc cgatcggctc
     2461 cgagcgctcc gacttctcgc tcgacatgag gctcgcgccg gggctgcgcg gccggctgaa
     2521 gctcatcctg cgctacctgc cgcccgacgg atcgacgcgc gcctacgagc tgcacggcgg
     2581 cgccggccac gtgacgctcg acccgcgctg gcaccaggcg gcggccacgt tcgtcgcctc
     2641 gggcttccgc cacatcctgg gcggcgtgga ccacttgctc ttcctgctgt gcctggtgct
     2701 gccgttccgg cgcatcggct ggacgctggt cgcggtgatc acctcgttca ccgtggcgca
     2761 ctcgatcact ctgatcgctg cggcgcgcgg catggtgccg cccggtacct ggttcgcgcc
     2821 gctggtcgaa gtgctgatcg ccgcctcgat cctctacatg gcactcgaga acatcgtgcg
     2881 cccgaacctg cgctggcgct gggcagtgac ggggcttttc gggctggtcc acggcttcgg
     2941 cttctcgttc ctgctgcaga gccagatgca gctcgcgggg gagcacctgc tgctctcgct
     3001 cctcgccttc aacgtcggca tcgagctcgg gcagctcgtg ttcatcggcg tggcgatccc
     3061 gctgctcttc ctgctgaccg aaaagctgcg cttcgacgcc cggctgacga gcgtcgtgat
     3121 ctcggcgttc gtcgcgcatg ccgcatggca ctggatgacc gagcgctggg acgagctcgc
     3181 gaagcagccc tggccggagg tcgacccgtg gcagcttgcg ctgtggtgcc tcctcgccgc
     3241 ggcgctcgcg gtgctcgcct gggcgctgct aggcagagtg tcggtattgc gacggcgtga
     3301 ggccgaaccg ggcgtgaaac cgcactgaga agtgcgccgg gctgcggaag ccgcaggcgc
     3361 gcgcgatgtg cgcgatggtg gccggaccct tgagcatcgc cgcggcgcgg tcgagccggc
     3421 gctcgagcgc gaagcggtgc ggcgtctttc cggtggcgcc gcggaaggcg cgcacgaagt
     3481 gatcgaccga caggtgcgcg agcgctgcca gtgcctgcag cggcacgtcg ctgcgctggt
     3541 tcgcctcgag gtaatcctcg atgcggcgga tcacgccgcg gccgagcggc gttacgcgca
     3601 ggcgcggctc tggcgtcctt ttcgaaacgt tcgagtagtg gcgcaccaga tggcgcagca
     3661 agaagtgctg cgtctgctgg aggaacaggg aatcgtcgta gagcgcacgc tcgctgtcga
     3721 gaaggcggca ataggcggcg agccagggat cggtaatgcc gaagaaatcg cggatgccgc
     3781 gcagcgcctc cggcgggata tagacgtgga tcgcctcgat cggcgcatcg ctcgaccact
     3841 cgaccgggca gtcgcccggc gagaacgtca ccgagccgat cggcggcacc ttgcgctgca
     3901 ccaccccgcg gcacgtgcgc acgatggtcg acatcccgct cacgcggtag ccgaggaggt
     3961 ggtgcggcgc ccagagattg ggcacgcggt gcggcgggac ccgccacttg gccgcagcca
     4021 gcccgccggt gccttcgagc aggaacaccg gctgcgcgcc cgtcgccgcg gcgtagcgcg
     4081 caggaatgcc gctcaactca gcggcagtgc tctcgatact tgtgctccgc atggtgcgac
     4141 ttggcccagc gcctgtcgtt cgggtcgtag accatcggcc ggaagtagtc gagcgggtcg
     4201 accatccagc gcagctccgg ccactggctc tccgggaagc ccatcgcgag cgcggtctcg
     4261 cgccagttga cgaggtagta gggcgaggag ccgcgctgcg cgaccgccga gaagtgcccg
     4321 gggacgaaga cgcggggctt caggcgctgg atgtagctga tgatgtcgcg ctgctggttg
     4381 ttgttcgcgc ccaggctgat caccgagccg agcattacgt cggtctgcgg caggtcctcc
     4441 atcagcgaga acaggccctt gccgatctcc gcggcgagcg cgatcgccgg cccgttgttg
     4501 accgggtcgt tgtactgcgc gattgagatc aggccgggcg agccgctgcc gatgccttcc
     4561 ttcaccggcc cggcagagtt gatgtaggag aaggcgaagt tgttgtcgcc gcgcagcacg
     4621 aagtgatagc cgatctcgat gatgccggcg gcgccgccga agccggtggt ggtggtgttg
     4681 agctgcccgg gcacgcgcga cgccgggttg ccgggcggcg tgtacgggat tccggtcggg
     4741 tagagcgccg ggtaggtcac cgccggcgtc gtgaacacgc gccccgcgtc gcgcggatcg
     4801 gcgaggtcgt tgagcgtcga atgggcgaac gacgggtcga ccggcgcgtt gcccgagtgg
     4861 atgtgcttga acgcgaggat gcagatgtcg cggtcgaact gcttgatgcg ccgcacgcgc
     4921 gtcgtgccgc cggtcgggtt ctcgagcgtg ccggagtatt ccccgggcgg cgagttgcgc
     4981 ggcacggcgc cgacgcagtc gaccgggtct ccgttcggga tgatcttcgc gccgccgttg
     5041 tgctcgttgg ggtcgttcca catccgcgtc acgtcctgct gcatcacgtc gcaggtctcc
     5101 ggcgaggcgt agatcgtcgc gccggtccac ttcgcgacgt aggcggcgtt gtcggcgtgg
     5161 tcgccgtggc cgtggccgag gaagatcgcc tccggcttga ggtcgacgaa atccttcggc
     5221 agcgccttgg tgcggcgccg gtcgatcggc ccgtcggtcg gcagctccac gtgcagcagg
     5281 tagctgtcga gcatcagcac gcggcccttg accgagacca cgtaggtggt gttggtcgac
     5341 cacgagaaaa tgaccttgtc ttcgttcacg cggccgcggc tgtcgacgtt ctcgacgccg
     5401 aagaacttct gccgcgcctt caccagcgcc gggtcgaccg gcttccagcc gccacggtcg
     5461 tcgcggtcgt cgtcgtcgcg gtcgtgggcc gctgcggcag ccgaaaaggc gccgagcgcg
     5521 cccgccagaa tcaccgccat ggttgtcttg cagttcatgc tgtccccttt attgagaaaa
     5581 aaagggaggc cgccaagcct ccctttcctg aacgaatgcc taccggtcag tcatcgtcat
     5641 cgtcgtcatc gtcatggcgg cggcggtcgg tcacgtcgaa caccaggttc tcgggcgacg
     5701 ggccgaacga ggcgaacggc accactttca cctgcttcac gcccttcttg gaccagtcgc
     5761 acacgccttt cgggaagatc tggctcagcc gcgcgcgctg ctgcgacgtg aaggccacct
     5821 tgtagtccga catgtcgatg cgcttgaggt ggcacttgat ggcgtcgagc tcgatcggcg
     5881 cgcccgccac gtactccggc ggcacgccgc gcgggaacag cacgttgcac tggcccgggc
     5941 cgtagagcac ctgcggctcg acgatcttca cgccggcggt ggtccagcag gcgtcgacca
     6001 cgtcggccgg cttgttggcc accaccttgg ccgcccggga gcggttcgac gtgtcggcga
     6061 ctaccgccgt gagccactgg cccatctgat cgatcagcag ctcgctcggc gcgaggttgc
     6121 cgacgatcac ctggttgttg gcgtggccgt tggcgttgat gatccgctgg cggatcgaga
     6181 acgagtggta gcgcagatgc acgtctcccg ccacgaggag gtcgttgccg cccgagcgcg
     6241 tgatgattgc cgtgctcgcg aggccgccgc cgccgctcag gatgcggccg ccctggtaag
     6301 cgcgcttcgt ggcatccggg tagtgcaccg tgcggttggg aacgcggttg aagtcgatgt
     6361 cgacgccgcc catgttggcg ttcaggtcga gaaagtcctc gacgctgatg acgccgtcgt
     6421 tcatcgctcc cagcccgtac tgcacgccga cgttgtccag cggacgctgc gcgaacttgg
     6481 aatcgccggg gaagggcgtg ttcctgatga cgccgtacac gttcaccgtg tggtcgtaga
     6541 cgtcgccgcg cgcgccgtac gggttggtca cggggtcgta gcggaactgc gggccgacgc
     6601 ccggcaggat cgcatccggg aacgcgaccc gcgggtccat ccggtcgcct cgctcccggc
     6661 tcatctcgtg gatctggccg gtcacgaaca ggtccgggtt gatcgacagg tatcccgtca
     6721 tcgccttcac ctgctccacc gtgtaccggc ccggacgcga ggccaggtag agctcgaaca
     6781 ggcgcgagct gtgcatcggg atcatcccgg tggtgacgtc cgggaagctg ttcatggtga
     6841 cgatgccgtc gaacgtgccg gggtagttgt cgccggtctg gttgctctgg taggcgccgc
     6901 ccgagctgcc ggtgccgatg gtgaacttcg gcacgccgta gctctcgatg aagcgctcct
     6961 tcaccatcag gatggtctcg gacgagagca ggtcgttgca gttgttgccg aaggtgttga
     7021 ggctcgccga ggcgaccgcg tggcccttgc ccagccaggt ggcgttgagg ggcgtgacga
     7081 ggccgtcgcc ctgcgtgtac cagccggcga tgcagccgcc gccgagcggg tagatgacgc
     7141 gcccgttcca ggcggcaggc ggcgccgccg ggctgggctc gccgctgttc gggttgtgca
     7201 ggatcgcggt ctggtagatg gcgcggttga tcgtcccggt ctcgagccgc acgatgaacg
     7261 gcaccgtggc gccggtcacc gtcgtggtca tggccagatc ggccggcggg gtcgagtacg
     7321 gctgcggcag cagcgcaaag gtgttgttgg tcttgcggta cacgtagttc acgcgcctcg
     7381 cgaccgagca gttcgcgtcg agcggcgcgc cgagcgccgc gccgcccggg tagggaacga
     7441 agtcttgggt ggtgcaggcg aacgggaact ggtgcgggcc ggagatgatc ggtccggtga
     7501 ccggatagtt ggtgagcttg agctccgcct cggcgacgcc gtggcgcttg ctgtctgcct
     7561 ccagccggtt gtcgcccagg cgcatgccgc cgacgacgcc ggtgagcgtt ttcgcgccgt
     7621 ggttggcgac gagcgcgccg gtgacgtttc gcccgttcag ctcgacgcgc acgtcgctga
     7681 ggcgcacgtc cttcttcttc acggtgacgc gcaccagcgc gtcgccgccg gacaccaggt
     7741 ggggcttgct ggagaggacc tcgatgcgga acgcgctcga gccgcggtgg cggcggtcgc
     7801 cgtcgtcatc gtcgtcgtcg tgcgcgtaga ccggactcac ggccaccgcg cacagcaacg
     7861 cgcacgcgga aaggcgcaga ttggttttgc tcatggttat tttcctttct gggtatcagt
     7921 ggacgagcat agccaccgcc gcgaaggcgg atgtttctgg atgcttctga gttgatcacc
     7981 gccgttcatc aatcggaggc gcagacaaaa aaagggaggc gcgatgcctc ccttcccgga
     8041 ctacagacgc tttacgtcag ctcggcatca atcgtcgtcg tcatcatcgt cgtcgcgatc
     8101 gccgcgcccg cgcgtcggcc actcgcggca ggtgtagttc gcggcgagcg cgatgtcccc
     8161 gcccgcgggg ccggtgtagc gcagcgtctg cgggtacggg cacagcggcc ggctgcgcgt
     8221 ggtgggcgcg gtcttgaagc tgacgccagt cgcgaccacg gcgtccggcg ccttgcgctc
     8281 ctcgacccac gccatgagcg gggtgaggaa atcgaagcgg tccgtcgacg gcccgccgct
     8341 gcaatggccc atgttgggca ccatgtaaag ccgcgcgaag tctcggtgcc ggctgtgcct
     8401 gccgtggtcg cgatccgtaa tgcggtcgta gaagtaggcg gtgtaggtcc acggcgggcc
     8461 ggagtcgctc aggccgtgat agaagatgat cttgccgccg cgccggcgga agtccgagag
     8521 gtcggtgtcg tcgcgcacgc cggcggcgcg cttgaccacc
//
LOCUS       DATBSZ010000037         6236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1097409, whole genome shotgun sequence.
ACCESSION   DATBSZ010000037 DATBSZ010000000
VERSION     DATBSZ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097409"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VIG70_02560"
     CDS             <1..660
                     /locus_tag="VIG70_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249459.1"
                     /translation="AVFIGHFAVAFAAKRAAPEVSLGTLFLAAQLADLVWPTLVLLGL
                     ERFEIRPGITAVTPLDFVHYPYSHSLVALAGWAFALGFLYYAFRRGGRGAAILFAVVL
                     SHWLLDFASHRPDMPLTIGGEERFGLGLWHSRMATLAVEGLLFGTCVWLYARTTRALD
                     RTGRWSFVAFVAFLIVVYVADVFGPPPPSVAAVAWSAQAIWLLVAWGYWIDRHRAASR
                     N"
     gene            complement(636..1490)
                     /locus_tag="VIG70_02565"
     CDS             complement(636..1490)
                     /locus_tag="VIG70_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8249460.1"
                     /translation="MTSTVVLDGIATRYEVLGDGPPLLMYAPGGFDATIEKWRAQGVY
                     ERVKLLEHLPKKYRCIAFDRRECGRSGGRVEQITWRHYAEQGKALLGHLGIKRAHLIG
                     GCMGCSCVIAFAVAYPEATQSMILWWPVGGAKYRIKSRARFTDHVAFVREHGLPASAA
                     LVRQEGKPFNADSRGGPWASLMRADPAFAASFPNMNRGVYTSIVKGMRDALFDRDTSP
                     GAVPEDLLKLDIPALVVPGRDESHATSAARYLEECLPKSQYWDALPEAQTEAAAAPRL
                     IQFLDAAR"
     gene            complement(1491..2201)
                     /locus_tag="VIG70_02570"
     CDS             complement(1491..2201)
                     /locus_tag="VIG70_02570"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY8249461.1"
                     /translation="MAVISDLQKMKQNGRKIVGVVAWDYPIAQIADYAGVDLVSVGDS
                     VGVNLWGQAPHEISIDEMLIVCRAVRRGVKRAVVSVDLPAHEQNAESALRLKDCGADM
                     VKLLGSPDLVRAVVRAGIPVFAEIHGGKDGPAMLVDHARRLQDAGASALDFRHSGPVA
                     GEAVARAVSIPVIGGLGGGPWLDGRLRMVHAAIGYGFSNLGKATDQYANVAQLTHDAL
                     AAYAEDVRAARQIKGQRA"
     gene            2226..3245
                     /locus_tag="VIG70_02575"
     CDS             2226..3245
                     /locus_tag="VIG70_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8249462.1"
                     /translation="MIIDIHGHYTTEPQALLAFRDKQLAGLADPMRKPATNELGITDE
                     QLIKSVEPQMKLQKERGSDLTIFSPRAAGMAHHVGTEATGVEWTRMSNDLIQRICTLL
                     PGNFAPVGQLPQHPGVPPKNCIPELERLAGLGFVGVNLNPDPAGGYWTDPPLTDKWWY
                     PLYEKLCELEMTAMIHVTSSCNPAFHYTGAHYINGDTTAFMQLVQGNLFRDFPALRFV
                     IPHGGGAVPFHWGRYRGLAQEMKKPLLKEHLLGNVFFDSCVYHLPGIELMAKVIPVDN
                     ILFASEMLGAVKGVDPETGHNYDDTKRYFDKLDLSASDKQKIFEGNARRVYPRLNSLL
                     KRRQK"
     gene            3323..4183
                     /locus_tag="VIG70_02580"
     CDS             3323..4183
                     /locus_tag="VIG70_02580"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY8249463.1"
                     /translation="MCEPFVVGARDVLEAQGLPLQKVRVIETRRREFHVGEIHPEHGR
                     AALDAARTAIEGALAGEYAAVVAAPHSETAIHAAGVAFDGYPSFVARTAGLAPEDGIL
                     MLCFRHAGREVRIAHVTLHSSVSTSLKLITKKRVLRTIRAVHEALKRLSYPRPRIAVS
                     GVNPHAGEGALFGDEETTAVTPAIEAARGEGIVVDGPIGADTLIQRTGYDAYVVMLHD
                     QGHVAAKLLAPNRTAALTIGTPVLFSSVAHGAAFDIAGRNRADPAAMVEAITRLVTRR
                     RRGPQTARSS"
     gene            4359..4727
                     /locus_tag="VIG70_02585"
     CDS             4359..4727
                     /locus_tag="VIG70_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249464.1"
                     /translation="MRQSLRRERTVFLLFLGASFVALMAALPIVTAQQTQATPKSEEI
                     QKPSAASGSSAPASPAPSTSEAPSFEQADRNKDGFVDKSESGVVPGLSANFERADRSR
                     DGKLDREEFAKGLQILQVRR"
     gene            4745..5500
                     /locus_tag="VIG70_02590"
     CDS             4745..5500
                     /locus_tag="VIG70_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249465.1"
                     /translation="MLDLRLVKTATDAETARRLAREALWLRSLGGVRSLEGQLPRLIE
                     EGTDAAGRPYLVIGIRPEGDRPAGEIRAFTDEHARFLATLGKARFRAADFEHFGKCQW
                     IQRSLPPHEPILQEAYYDCEKALLYWNGPYVLSQGDFAPWNIRAFGSQLFVFDWGSAH
                     GDASPLDDVLHYLMIQRRLKTVPLLRRAMGRAREFALAAYPEWSWRPPVIGALTLVYL
                     LGVIVHREAKRSDPVVDAYWKLLEKRSAWMKEN"
     gene            5504..5740
                     /locus_tag="VIG70_02595"
     CDS             5504..5740
                     /locus_tag="VIG70_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249466.1"
                     /translation="MSAAATAAGYLYASGRLRGRTAREQEEGPKEIRKKPQKPAPRSR
                     ERIRVAVYQPEPRVQEQKKVPKIEYRTRRRWSAE"
     gene            5737..>6236
                     /locus_tag="VIG70_02600"
     CDS             5737..>6236
                     /locus_tag="VIG70_02600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8249467.1"
                     /translation="MSARSGGLFVGRLLAENGIGTLVEALDLYPGARIDVIGTGLDEG
                     RLAGHPRIRVLGRVGPAEVQERMRAAAYLVLPSLSYEALPRPVVEAFANALPVIASRI
                     GRLAEIVEPGRSGLLFEAGSARDLARRLAWAEAFPEKMRQMGECAKADYQAHYLADWS
                     YQRLFG"
BASE COUNT          995 a         2149 c         2115 g          977 t
ORIGIN      
        1 gcggtgttca tcgggcactt cgcggtcgcc ttcgccgcga agcgcgcggc gcccgaggtg
       61 tcgctcggca cgctgttcct cgcggcgcag ctcgccgatc tcgtctggcc gacgctcgtg
      121 ctgctggggc tggagcgctt cgagatccga cccggcatca ccgcggtcac gccgctcgac
      181 ttcgtccact acccgtactc gcacagcctc gtagcgctgg caggatgggc ttttgccctg
      241 ggtttcctgt attacgcatt caggagagga ggacgcggcg cggcgatcct cttcgcagtc
      301 gtgctcagtc actggctgct cgacttcgca agccaccgcc ccgacatgcc gttgaccatc
      361 ggcggcgagg agcgcttcgg gctcgggctg tggcattcgc gcatggcaac gctggcggtc
      421 gaggggctgc tgttcggcac ctgcgtgtgg ctctacgcgc gcaccactcg cgcgctggac
      481 cgcaccggcc gctggtcgtt cgtcgccttc gtggcatttc tcatagtcgt gtacgtggcc
      541 gatgtcttcg gcccgccgcc gccgagcgtg gcggcggtgg cgtggagcgc gcaggcgatc
      601 tggctactgg tggcgtgggg ctactggatc gaccgtcatc gcgcggcgtc gaggaactga
      661 atgaggcgcg gggcggctgc agcttcggtc tgcgcctcgg gcagcgcgtc ccagtactgc
      721 gatttcggca ggcactcctc gaggtagcgc gcggccgagg tcgcgtgcga ctcgtcgcgg
      781 ccggggacga cgagggcggg gatgtcgagc ttcagcaggt cctccggcac ggcgcccggc
      841 gaggtgtcgc ggtcgaacag cgcgtcgcgc atgcccttga cgatgctcgt gtagaccccg
      901 cggttcatgt tcgggaagga agcggcgaac gccggatcgg cgcgcatcag cgacgcccac
      961 ggcccgccgc gcgagtcggc gttgaagggc ttgccttcct gccgcaccag ggccgccgac
     1021 gccggcaggc catgctcgcg cacgaacgcc acgtggtcgg tgaagcgcgc gcgcgacttg
     1081 atccggtact tggcgccgcc gaccggccac cacaggatca tgctctgcgt cgcttcggga
     1141 tacgcgaccg cgaatgcgat cacgcacgag cagcccatgc agccgccgat gaggtgcgcg
     1201 cgcttgatgc cgaggtgccc gagaagcgcc ttgccctgct cggcatagtg ccgccaggta
     1261 atctgctcga cgcggccccc cgagcggccg cactcgcggc ggtcgaaggc gatgcagcgg
     1321 tatttcttcg gcaggtgctc gagcagcttg acgcgctcgt agaccccttg cgcgcgccac
     1381 ttctcgatgg tggcgtcgaa gcccccgggc gcgtacatga ggagcggcgg tccgtctccc
     1441 aggacttcgt agcgcgtggc gatgccgtcg agaacgacgg tacttgtcat ttatgcgcgc
     1501 tgccctttga tctgccgcgc ggcgcggaca tcctcggcgt acgcggcgag agcgtcgtgc
     1561 gtgagctgcg cgacgttggc gtactggtcg gtggctttcc ccaggttgga aaatccgtag
     1621 ccgatcgcgg cgtggaccat gcgcaggcgg ccgtcgagcc acgggccgcc gccgagcccg
     1681 ccgatcacgg ggatgctcac cgcgcgcgcc accgcttcgc ccgcgaccgg ccccgagtgc
     1741 cggaaatcga gagccgaagc gcccgcgtcc tgcagccgcc tcgcgtggtc gacgagcatc
     1801 gccgggccgt ccttgccgcc gtgaatctcg gcgaacaccg ggatcccggc gcgcaccacc
     1861 gcgcgcacca ggtcgggcga gccgagcagc ttcaccatgt cggcgccgca gtccttcagg
     1921 cgaagggcgg actcggcgtt ctgctcgtgc gccggaagat cgacgctgac gaccgcgcgc
     1981 ttcactcccc gccgcaccgc ccggcacacg atgagcattt catcgatgct gatctcgtgc
     2041 ggggcctggc cccagaggtt gacgccgacg ctgtcgccca ccgaaaccag gtcgacgccc
     2101 gcgtaatcgg cgatctgcgc gatcgggtag tcccacgcga ccacgccgac gatcttgcgg
     2161 ccgttctgct tcatcttctg gagatcggaa atcacggcca cggtcaatgc tagcattccg
     2221 ctgcaatgat cattgacatc cacgggcact acacgaccga gccgcaggcg ctgctcgcgt
     2281 tccgcgacaa gcagctcgcc ggcctggccg atccgatgcg caagcccgca acgaatgagc
     2341 tggggattac ggacgagcag ctgatcaaga gcgtcgagcc gcagatgaag ttgcagaagg
     2401 agcgcgggtc ggatctcacg atcttctcgc cgcgcgccgc gggcatggcg caccacgtcg
     2461 gcaccgaggc gaccggcgtc gagtggacgc ggatgtcgaa cgacctcatc cagcgcatct
     2521 gcacgctgct gccggggaac ttcgcgccgg tgggccagct gccgcagcac ccgggcgtgc
     2581 cgccgaagaa ctgcatcccc gagctcgagc gcctggcggg cctgggcttc gtcggcgtga
     2641 acctgaatcc cgaccccgcg ggcggctact ggaccgatcc gccgctcacc gacaagtggt
     2701 ggtacccgct gtacgagaag ctctgcgagc tcgagatgac ggcgatgatc cacgtcacct
     2761 cgtcgtgcaa cccggcgttc cactacaccg gcgcgcacta catcaacggc gacaccaccg
     2821 ccttcatgca gctcgtccag ggcaacctgt tccgggattt cccggcgctg cggttcgtca
     2881 tcccccacgg cggcggcgcg gtgccgttcc actgggggag atatcgcggc ctcgcgcagg
     2941 agatgaagaa gccgctcctc aaggagcacc tgctcggcaa cgtgttcttc gacagctgcg
     3001 tgtaccactt gccgggcatc gagctgatgg cgaaggtgat cccggtcgac aacattctgt
     3061 tcgcctccga gatgctcggc gccgtgaagg gcgtcgatcc ggagaccggc cacaattacg
     3121 acgacacgaa gcgctacttc gacaagctcg acttgagcgc ttccgacaag cagaagatct
     3181 tcgaaggcaa cgcgcggcgg gtctacccgc gcctgaactc gctattgaaa agaaggcaaa
     3241 agtagcgatc gcgatcggcg accccggcgg catcgggccg gaggtctcgc tcaaggcggc
     3301 gctcgacccc agagtccgcg cgctgtgcga gccgttcgtc gtcggagcgc gggacgtgct
     3361 cgaggcgcaa ggcctgccgc tgcaaaaggt tcgcgtcatc gagaccaggc ggcgcgagtt
     3421 ccacgtcggc gagatccatc ccgagcacgg ccgcgccgcg ctcgacgcgg cgcgcaccgc
     3481 gatcgaaggc gcgcttgccg gcgagtacgc cgcggtggtg gcggcgccgc acagcgagac
     3541 cgcgattcac gccgccggcg tcgccttcga cggctatccg tcgttcgtcg cgcgcactgc
     3601 tggcctcgcg cccgaggacg gcatcctgat gctctgcttc cggcacgcgg ggcgcgaggt
     3661 ccgcatcgcg catgtcacgc tgcattccag tgtttcgact tcattgaagc tgattacgaa
     3721 aaagcgggtg ctgcgaacga tccgcgcggt gcacgaggcg ctgaagcggc tctcctaccc
     3781 caggccgagg atcgccgtca gcggcgtcaa cccgcatgcc ggggagggcg cgctcttcgg
     3841 cgacgaggaa acgacggccg tgacgccggc gatcgaggcg gcgcgcggcg aaggaatcgt
     3901 ggtcgacggc ccgatcggcg ccgacacgct gatccagcgc acgggctacg acgcgtacgt
     3961 ggtgatgctg cacgaccagg gccatgtcgc ggcgaagctg ctcgcgccca accgcaccgc
     4021 cgccctcacc atcggcacgc cggtcctgtt ctcctcggtg gcgcacggcg cggctttcga
     4081 catcgcgggg cgcaatcgcg ccgatcccgc agcgatggtg gaagcgatta cacggttagt
     4141 gactcgtcga cgtcgcggcc cgcagaccgc aagaagctca tagaaacaac gctttagagc
     4201 agagccggcc acaaaggtaa cgccagcccg acgcgcctgg cgttcgcgcc gcgatctgcg
     4261 gtggtctaac tacttgtcgc agcggggatt cgcgccattg gcacgcggct tgcgagagcc
     4321 gtcgacacac aagtgctaac tcgaagccgg agccacccat gagacagagc cttcgccgcg
     4381 agcgaaccgt gttcctgctg ttcctcggcg cgagcttcgt cgcgctgatg gccgcgttgc
     4441 cgatcgtcac tgcgcagcag acccaggcga ccccgaaaag cgaagaaata cagaagccct
     4501 cggcagcgtc cggctcgtcc gctcccgctt ctccagcccc ttccacgagc gaagccccga
     4561 gcttcgagca ggccgaccgc aacaaggacg gtttcgtcga caagtcggaa tcgggcgtgg
     4621 tcccgggcct gtcggccaac ttcgagcgcg ccgaccgcag ccgggacggc aagctcgacc
     4681 gcgaagagtt cgcgaagggg ctccagatcc tgcaggtgcg ccggtagccg ggacgaggat
     4741 gccgatgctc gatcttcgcc tcgtcaagac cgcgaccgac gccgagacgg cgcggcggct
     4801 cgcgcgcgag gcgctgtggc tgcgcagcct cggcggcgtg cgcagcctcg aaggccagtt
     4861 gccgcggctg atcgaggaag gcacggatgc ggccggtcgg ccgtacctcg tgatcggcat
     4921 tcgacccgag ggcgatcggc cggcgggcga gattcgcgca tttaccgacg agcacgcgcg
     4981 cttccttgcc acgctcggca aggcccgctt ccgcgccgcg gatttcgagc acttcggcaa
     5041 gtgccagtgg atccagcgat cgctgccccc ccatgagccc attctccagg aggcgtacta
     5101 cgactgcgag aaggcgctgc tgtactggaa cggcccgtac gtgctttcgc agggcgactt
     5161 cgcgccctgg aacatccgcg ctttcggctc gcagctcttc gtcttcgact ggggcagcgc
     5221 gcacggcgac gcgagccccc tggacgacgt gctgcactac ctgatgatcc agcgccggct
     5281 gaagaccgtg ccgctcttgc gccgcgcgat gggccgcgcg cgcgagttcg cgctcgccgc
     5341 ctacccggaa tggtcctggc ggccgccggt gatcggcgcg ctcaccctgg tgtacctgct
     5401 cggcgtgatc gtgcaccgcg aggcgaagcg cagcgacccg gtggtcgacg cgtactggaa
     5461 gcttctcgaa aagcgctcag cctggatgaa ggagaactaa gaaatgtcgg cagcagctac
     5521 cgccgcgggt tacctctacg cttccggccg cttgcgcggc cgcaccgcgc gcgagcagga
     5581 agagggcccc aaggagatcc gcaagaaacc gcagaagccc gcgccgcgtt cccgcgagag
     5641 aatcagggtc gccgtttatc aacctgaacc ccgggtccag gagcagaaaa aagtcccgaa
     5701 gatcgagtac cgaacgcgcc gtcgctggtc ggcggaatga gcgctcgctc gggaggcttg
     5761 ttcgtcgggc gcctgctggc ggaaaacggc atcggcacgc tcgtcgaagc cctcgatctc
     5821 tatcccggcg cccgcatcga cgtgatcggc accggcctgg acgagggtcg cctcgccggc
     5881 catccgcgca ttcgcgtgct cggccgcgtc ggcccggccg aggtccagga gcgcatgcgc
     5941 gccgctgcct acctcgtgct gccaagcctc tcctatgaag cgctgccgcg gccggtggtc
     6001 gaggctttcg ccaacgcgct gccggtcatc gcgagccgca tcggccgcct cgccgagatc
     6061 gtcgagccgg ggcgcagcgg gcttctgttc gaggccggct cggcgcgcga cctggcgcgg
     6121 cgcctggcgt gggcggaggc atttcccgag aagatgcgcc agatggggga gtgcgccaag
     6181 gcagactacc aggcgcatta cctcgcagac tggagctatc agagactctt cggcga
//
LOCUS       DATBSZ010000038         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2395707, whole genome shotgun sequence.
ACCESSION   DATBSZ010000038 DATBSZ010000000
VERSION     DATBSZ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2395707"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VIG70_02605"
     CDS             complement(<1..825)
                     /locus_tag="VIG70_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8249468.1"
                     /translation="MNKPAELKLETVPVWINGKAVAPAGRTGEVFNPATGQVSRKVVF
                     ADAKLIDAAVSAAAAAFPEWRDTPPLRRARVMQKFLSLLQKNQTQLAQIVTDEHGKTL
                     ADAMGSVQRGIEVVEFACGIPQLLKGEYSQDVGTQVDTHTIRQPVGVCAGITPFNFPV
                     MVPLWMFPMAIACGNTFILKPSEKVPSASIRMAELFKEAGLPDGVFNVIHGDKVAVDG
                     LLSHPEVKAISFVGSTPIAKYIYETAAKNGKRVQALGGAKNHAVVLPDADLEFAADA"
     gene            complement(822..1946)
                     /locus_tag="VIG70_02610"
     CDS             complement(822..1946)
                     /locus_tag="VIG70_02610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8249469.1"
                     /translation="MKLGMFMMPMHALHRSHTETLREDREAVILADQLGFHDAFVGEH
                     LTDKAENVTNSLQFLATLIHSTKKIKLGTGTCNLAQQHPVQVAAQAAMFDHLAEGRFI
                     FGISPGTLASDAEAIGILDQDRMKMFAEAIDVILAIWERDPPYDIEFPGNRYKVTTRK
                     TLHLELGTGVMARPYQKPRPEIVGTVVAPYSKGVVHMGERDFHPLSANFLLSNWLKSH
                     WDNYAEGKAKAGVKADPAEWRIARTIFVADNDKVAASYGRHDSNSPYRFYYNQIQTKL
                     LRAGRIAGFKERMDQPDAEITYDFVLDRLCIYGTVNKVVDHILELREKTGDFGELVIA
                     AMDWVDPKLEKRSLQLMAEQVMPRVNAAIAKEKGGKRVTA"
     gene            1970..3736
                     /gene="mnmC"
                     /locus_tag="VIG70_02615"
     CDS             1970..3736
                     /gene="mnmC"
                     /locus_tag="VIG70_02615"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEY8249470.1"
                     /translation="MPEALVPATLAFQEGTPYSEAFRDVYHNVDGGIAKKRHVFLAGN
                     SLPERWAGRRRFVILETGFGLGLNFLVTLQAWRRDPARSERLHFVSVEKHPFGLADLQ
                     AAHAAYPEVRDEAAELHARWPLLTPGMHRLEFGNAVLTLLFADIKALRDLRLAADAIY
                     LDGFSPARNGDMWSPAVLRAISRLAAPGATAATWSVAGAVRHALEETGFAVEKRPGFG
                     NKKEMLVARNTRPGKVREAPTERRAVVVGAGLAGAAVCERLCARGWEVELYERHAEPA
                     QEASGNHAGTFHPIVTPDDSIFARLTRASFLFSNSYWKSFSGIRWDPCGVLQLARDDR
                     EEASQRASVAALALPPGYAQYVTREEASAHAGVPVGAPGLWFPEGGWIQPRSLVQAQL
                     EACGNRLKTFFRKSLSQLPDVPAVILANSAEAPKLQDVPHLRMRRVRGQVSHLAADAI
                     DAPHVVVLRGGMILPPVGGLCVVGASFDVDDPDPAPRAESDAGNLERLGRIIPGIGTP
                     PVVQNRVAFRAVTPDRLPVVGKLGEGVYGAFAFGSRGLIWAALAAEIIACELEGEPLP
                     IEGKLADAMHPQRFAKRAARFV"
     gene            complement(3733..>3892)
                     /locus_tag="VIG70_02620"
     CDS             complement(3733..>3892)
                     /locus_tag="VIG70_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEY8249471.1"
                     /translation="TMIGRAYAYGLGALGEAGVTRALEIIRAELDVTMGLCGLRDVKD
                     ASPAILRG"
BASE COUNT          572 a         1279 c         1342 g          699 t
ORIGIN      
        1 cgcatcggcg gcgaactcga ggtcggcgtc gggcagcacc acggcgtggt tcttcgcccc
       61 gccgagcgcc tgcacgcgct tgccgttctt cgccgcggtc tcgtagatgt atttcgcgat
      121 cggggtggag cccacgaacg atatcgcctt cacttcgggg tgggaaagca ggccgtccac
      181 ggccaccttg tccccgtgga tcacgttgaa cacgccgtcg ggcaggccgg cttccttgaa
      241 gagctccgcc atgcggatcg aggccgaggg caccttttcc gatggcttca ggatgaaggt
      301 gttgccgcag gcgatggcca tcgggaacat ccacagcggc accatcaccg gaaagttgaa
      361 cggcgtaatc ccggcgcaga cgccgaccgg ctggcggatg gtgtgcgtgt cgacctgcgt
      421 gccgacgtcc tgcgagtact cgcccttcag gagctgcggg atgccgcagg cgaactcgac
      481 cacctcgatg ccgcgctgta ccgagcccat cgcgtcggcg agcgtcttgc cgtgctcgtc
      541 ggtgacgatt tgcgccagct gggtttgatt cttttggagt aacgacaaaa acttctgcat
      601 gacccgggcg cggcgcagcg ggggcgtgtc gcgccattcc ggaaaggccg ccgcggcggc
      661 gctcacggcg gcatcgatga gcttcgcatc tgcgaaaacc actttcctgc tcacctggcc
      721 ggtcgcggga ttgaacactt caccggtcct gcctgcgggc gcgacggctt tgccgttgat
      781 ccagacaggg acggtttcca gtttcagctc tgcgggcttg ttcatgcggt gactcgcttt
      841 ccaccttttt ccttggcgat cgccgcgttg acccgcggca tcacttgctc ggccatgagc
      901 tgcagcgagc gcttctcgag cttgggatcg acccagtcca tcgccgcgat caccagctcg
      961 ccgaaatcgc cggtcttctc gcgcagctcc aggatatggt cgactacctt gttgaccgtg
     1021 ccgtagatgc acaggcggtc gaggacgaag tcgtaggtga tctcggcatc gggctggtcc
     1081 atccgctcct tgaagcccgc gatgcgcccc gcccgcagca gcttggtctg gatctggttg
     1141 tagtagaagc ggtaggggct gttggaatcg tgccggccgt aggaagccgc aaccttgtcg
     1201 ttgtcggcga cgaagatggt gcgcgcgatg cgccactccg ccgggtcggc tttcacgcct
     1261 gccttcgcct tgccctcggc gtagttgtcc cagtgggatt tcagccagtt cgacagcagg
     1321 aagttcgccg acagcggatg gaaatcgcgc tcgcccatgt gcaccacgcc cttcgagtac
     1381 ggcgccacca ccgtgccgac gatctcgggg cgcggcttct ggtagggcct ggccatgacg
     1441 ccggtgccga gctcgaggtg cagcgtcttt cgcgtggtca ccttgtagcg gttgccggga
     1501 aactcgatgt cgtaaggcgg atcgcgctcc cagatcgcga ggatcacgtc gatcgcctcg
     1561 gcgaacatct tcatgcggtc ctggtccagg atgccgatcg cctcggcatc ggaggcgagc
     1621 gtgccggggc tgatgccgaa gatgaagcgg ccctcggcga ggtggtcgaa catcgcggcc
     1681 tgcgccgcga cctgcaccgg gtgctgctgc gcgaggttgc aggtgccggt gccgagcttg
     1741 atcttcttcg tcgagtggat cagcgtggcg aggaactgca gcgagttggt gacgttctcg
     1801 gccttgtcgg tcaggtgctc gccgacgaag gcgtcgtgga agccgagctg gtcggcgagg
     1861 atcacggcct cgcggtcctc gcgcagcgtc tcggtgtggc tgcggtgcag cgcgtgcatc
     1921 ggcatcatga acatgcccag cttcatccgg ccgattctat actcgcgcga tgcccgaggc
     1981 tctcgttccg gcgacactgg cgttccagga aggcacgccg tactccgagg ccttccgcga
     2041 cgtctaccac aacgtcgacg gcggcatcgc caagaagcgc cacgtgttcc tcgccggcaa
     2101 ctcgctgccc gagcgctggg ccgggcgccg gcgcttcgtc atcctcgaga ccggcttcgg
     2161 cctcggcctc aacttcctgg tgacgctgca agcctggcgg cgcgacccgg cgcgcagcga
     2221 gcggctgcat ttcgtctcgg tcgagaagca tcccttcggc ctggccgacc tgcaggcggc
     2281 gcacgcggcg tatccggagg tccgcgacga ggcggccgag ctgcacgcgc gctggcccct
     2341 gctcaccccg ggcatgcatc gcctcgagtt cggcaacgcc gtgctgaccc tgctcttcgc
     2401 cgacatcaag gcgctccgcg acctgcggct cgcggccgac gcgatctacc tcgacggctt
     2461 ttcgccggcc cggaacggcg acatgtggtc gcccgcggtg ctgcgcgcga tctcgcggct
     2521 cgccgcgccg ggcgccaccg cggccacctg gagcgtcgcc ggcgcggtgc gccacgccct
     2581 ggaggagacc ggcttcgcag tcgagaagcg ccctggattc ggcaacaaga aggagatgct
     2641 ggtcgcgcgc aacacccggc caggaaaagt gcgggaggcc cccacggaaa gaagggcggt
     2701 cgtcgtgggc gccgggctcg ccggcgccgc ggtgtgcgag cgcctgtgcg cgcgcggctg
     2761 ggaggtggag ctctacgagc ggcatgccga gccggcgcag gaggcttcgg gcaaccatgc
     2821 cgggacgttc cacccgatcg tgactccgga cgacagtatc ttcgctcgac tgacacgcgc
     2881 tagctttctt ttttccaatt cttattggaa aagtttttca ggcattcgct gggatccttg
     2941 cggggttctc cagcttgccc gcgacgacag ggaggaagcg tcgcagcgcg cctcggtcgc
     3001 ggcgctcgcg ctgccgcccg ggtatgcgca gtacgtcacg cgcgaggagg cgtcggcaca
     3061 cgcgggcgtg ccggtcgggg cgcctgggct ttggttcccc gaaggcggct ggatccagcc
     3121 gcgctcgctg gtgcaggcgc agctcgaggc ttgcggcaat cgcttgaaaa cttttttcag
     3181 gaagagcctc tcgcagctgc ccgacgtgcc ggcggtgatc ctcgcgaact cggcggaggc
     3241 gccgaagctg caggacgtcc cgcacctgcg catgcggcgc gtgcgcggcc aggtcagcca
     3301 cctggcggcc gacgcgatag atgcgccgca cgtcgtggtg ctgcgcggcg ggatgatcct
     3361 gccaccggtg ggcggcctct gcgtggtggg cgcgtcgttc gatgtcgacg accctgatcc
     3421 cgcgccgcgc gcggaaagcg atgccggcaa cctggaaagg ctcggaagaa tcattccggg
     3481 gattggcacg cctcctgtgg tgcaaaaccg cgttgcattt cgcgcagtga cgcccgatcg
     3541 gctgccggtg gtcgggaagc tcggcgaagg cgtctacggc gcgttcgcgt tcggctcgcg
     3601 cgggctgatc tgggcggcgc tggccgccga gatcatcgcc tgcgagctcg aaggagagcc
     3661 gctgccgatc gagggaaagc tggcggatgc aatgcatccg caaaggtttg ctaagcgcgc
     3721 agcgcgcttt gtttagccgc gcagaatcgc cgggctggcg tccttgacgt ccctgaggcc
     3781 gcagagtccc atggtgacgt cgagctcggc gcggatgatc tcgagcgcgc gcgtcacgcc
     3841 cgcctcgccc agcgcgccca ggccgtaggc gtaggcgcgc ccgatcatcg tc
//
LOCUS       DATBSZ010000039        12359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_4790, whole genome shotgun sequence.
ACCESSION   DATBSZ010000039 DATBSZ010000000
VERSION     DATBSZ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4790"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VIG70_02625"
     CDS             complement(<1..956)
                     /locus_tag="VIG70_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY8249472.1"
                     /translation="MILDRKPLPERFCNFERLLAGMAARGLDGIVASTPLNVFYLSGF
                     NGIAHKADEPRPYALVLSRHAPEHPILVIADYYLATFLAQPSWVRDIRPFRAVMMPID
                     RAPQREDIERFIPQEAKQLEWVRAARSRYAFDKASALRGALADLGLEHGKVAEDANGG
                     EAYDVLMYARAVKTPTELELLRRATALNRAAIERTVKSWRKGMRWRELNRAYHLAAID
                     LGGFVRDPGAMVWGHPRGAEAALTLQTGLEDFEVEAGMHVMFDCHGTLDLYCWDGGKT
                     WVVGGEPQAEGRRNLRATAQVAEALVSEMKPGARPSELQARGR"
     gene            complement(953..2119)
                     /locus_tag="VIG70_02630"
     CDS             complement(953..2119)
                     /locus_tag="VIG70_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY8249473.1"
                     /translation="MKSESPLTARAAVRALTASKIRELYNEGLGNPNVLPFWVGEPDE
                     PTPDFIRKAGTDSIGAGELFYTHNLGIPELREALAAYVSKLHRPVDFQQIAVTSAGVN
                     ALMVASQLLVDPGDRVVQVVPLWPNLQEIPKILGATVETVALQFSPSGWKLDLDRLLG
                     ALTAKTRALYLNSPNNPTGWTVSAAELKAILEHCRRHGIWIFADDAYERLYYGDEGVA
                     PSLLNISETEDRLISANTFSKSWLMTGWRLGWLVVPPGLTADLGKLIEYNTSCAPVFV
                     QRAGIAAVKEGEPVIARTRYRFRKARDFLVSELKEIPQIEVAVPTGTMYTFFKVDGVT
                     DSLAFCKKLVRETGLGLAPGSAFGPEGEGFVRWCFAASEEKLADGVARLKRALQ"
     gene            2118..3050
                     /locus_tag="VIG70_02635"
     CDS             2118..3050
                     /locus_tag="VIG70_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8249474.1"
                     /translation="MSRPILDWSLIAAVCTMVAWGMNFAFVKYVLDYLGVGAFMFLRF
                     TILPLLGLVLLVLVFRHRIRHTWPRRADLPRFVLCALIGHVLHISAVMYGMNLSTAFS
                     SSLVLTCQPLFTLAILAWLGTERLRRQQIAGTLIAFAGIALFLADKFAAGFSRAGLGD
                     LTLLIAGAAFSAYTVLSRPLVDRYGPLIVLSYTLLFSMPVMLAVTWPFFAELKAVHLG
                     PMVWAAIFWALVVCSFCGWLVWTWVNSVRGVARSAPFMYLMPPVAGVMAWLTLGETFT
                     WLKIAGAAITMAGVAWAQFGGRPPPKEAGQPDSG"
     gene            3047..4210
                     /locus_tag="VIG70_02640"
     CDS             3047..4210
                     /locus_tag="VIG70_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8249475.1"
                     /translation="MKIPRTVWALGAVSLLMDLSSELVHSLLPLFMVTVLGASMLTVG
                     VVEGIAEATASIVKLFSGAISDRFGRRKPIMVIGYGLAAISKPLFPLAASVPLVLAAR
                     FMDRIGKGIRGAPRDALIADVTPEGMRGAAYGLRQALDTVGAVLGPLAAIGLMLLLAS
                     DVRSVLWWAIVPAFLSVVVLIVFVKEPEGAVRPLERFSLQSFNRLGVQTWAIVALGAV
                     LTLARFSEAFLVLRAQDLGVALALVPIVLVAMNVAYTAVSYPAGLAADHGHRKALLLW
                     GLLALIAADLVIAATASVPLLFLGIVLWGVHMGLTQGLLSTLVAAAAPHNLRGTAFGV
                     FHLASGVAMLLASVIAGALWDGYGAAATFYAGAGFTGFALLGLLLVRPQTATK"
     gene            complement(4181..4777)
                     /locus_tag="VIG70_02645"
     CDS             complement(4181..4777)
                     /locus_tag="VIG70_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8249476.1"
                     /translation="MMPGRVVYVVDDDDAVRNAVSLLFRTADLQVETFSSASAFLERA
                     NLERRCCVLLDIRMPGMTGTDLHEELLGRGVRAPVIFITGHGDIPMAVEAMKKGAYDF
                     IEKPFDDDHLLGQVLGALEDYDEETERHEPEKAPLAALSARQRAVLERVLEGKPNRQI
                     AEELSISLKTVEFHRARIMQKLGVRTAAELFRRCLGAN"
     gene            complement(4774..5775)
                     /locus_tag="VIG70_02650"
     CDS             complement(4774..5775)
                     /locus_tag="VIG70_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8249477.1"
                     /translation="MLAIDETAMALLSALVEALPFPVYVQEIDGGSFLYGNRRYLEIF
                     TTPPELEAEGEFHDAANDRWYVLRAHPLSWINGRKASLCILSDVTEERRARELIAKHR
                     DAAHRTARLIALGEFASAVAHELNQPLAAIATYNHSCMRMLQSGNPDPEQLLQAMHIC
                     RDQAKRAGRIIQRLRGLLRHPGPALAEQDLNELVAAVLELAEPGARDAGVSFERALSA
                     RPPVVRADRLLIEQAALNLVRNAVEAVQELAPERRRITVATRVELDGSGTLAVSDLGE
                     GVLPEVRERLFDAFVTTKRGGLGLGLSICRSVVEAHGGQIRLDHNGRGARFAFTLPR"
     gene            5844..6086
                     /locus_tag="VIG70_02655"
     CDS             5844..6086
                     /locus_tag="VIG70_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249478.1"
                     /translation="MGDPDNRLSRALEGFDLLLAAATALVRAKSARPALLDAYDDASD
                     RLMEGLRGNGVPGHELQAVHKALARLRLAFEQTPPS"
     gene            complement(6079..7074)
                     /gene="ltaE"
                     /locus_tag="VIG70_02660"
     CDS             complement(6079..7074)
                     /gene="ltaE"
                     /locus_tag="VIG70_02660"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEY8249479.1"
                     /translation="MIDLRSDTVTRPSPGMRKAMNDAELGDDVFGDDPTVNQLQARAA
                     EIFGFESALLFPSGTQSNLAALMSHCQRGDEVILGMEAHSYRYEAGGLSVLGSIQPQA
                     IPNRPDGTLDLAEVEAAIKPDDPHFARTRLLALENTITGRVLPRKYLQDAIELAKRKN
                     LSVHLDGARVFNAATQLRMKVKDLCAGFDSVSSCLSKGLGAPAGTVLLGNKPLIEKAK
                     RARKILGGGMRQAGVIAAAGLYALENNVERLADDHANAEKLARGLRELKLEVQHNTNM
                     VLVKIPPEKAQPLEAHMKKSGILVLPRAPMRLVTHLDVDAAGIDRALAAFRGFFS"
     gene            complement(7071..7835)
                     /locus_tag="VIG70_02665"
     CDS             complement(7071..7835)
                     /locus_tag="VIG70_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY8249480.1"
                     /translation="MKQRTHQSLERGLRLVEAVALNGVQTTLAEAARRTGLHRSTAHH
                     LLQTLVSLGYLRQDADTRAYELAAKPFQITGRTWSPEQLAGIAQTSLAELTRRSGEGS
                     SFSVYRDGAVTVVAKREHDGPVRVVQDVGARRPLHCTAVAKAILPWLAAPELAGLLAR
                     IRFERHTPKTIVTRAAFETELRRIRSAGYAVDDEEHIEGIRCIAMPVFGHTGQVLGSI
                     CTLGPKSRMTYQRLRDLRAPLGELSAKLSQRLGFNQ"
     gene            8112..9092
                     /locus_tag="VIG70_02670"
     CDS             8112..9092
                     /locus_tag="VIG70_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEY8249481.1"
                     /translation="MLINCVAYQEGRRLADIELREIHAHLAQPGTFVWVGLRDPTQAE
                     LATMQEQFDLHDLAVEDAGHGHPAVARPKVEEYGDALFVVMNAVELSPTDEIKLGEVG
                     VFVGRNYVLSVRRRSDRGFQEVRARAEREPDLLRHGPGYVLYALMDAVVDRYFPLLDA
                     IEVELEAIEERLFTPGASPRENIESLYYVKQRLTTIKHAAGPLLESTARLFGGRVPPV
                     CAGLGEYFRDVHDHLVRINQSIDAARETVGTAIQVALAMVATGQGEIVRRLAAYAALV
                     AVPTMIVGVYGMNFAHMPELKWTFGYPLVLGAIAAIDAFVFYRFRKAGWL"
     gene            complement(9107..10960)
                     /locus_tag="VIG70_02675"
     CDS             complement(9107..10960)
                     /locus_tag="VIG70_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEY8249482.1"
                     /translation="MPARQSTPALTLIALGVVFGDIGTSPLYAVKETFSAEHGIALTH
                     ENILAGLSLMFWSLMTVVSLKYVMLIMRADNRGEGGIMALIALATQAIRAQPHWRLPL
                     LIIGVFGASLFYGDAVLTPAISVLSAVEGLEVGTGALKPFVVPIAVGVLAALFALQSR
                     GSEAVGTLFGPVTLLWFLAIAAAGIHGIAREPAVLAALNPLHALRFVMTHAVESFVVL
                     GAVVLVVTGAEALYADMGHFGRRPVRIAWFGLVAPALVLNYFGQGALLMTEPAAVSNP
                     FYLLLPGWALYPMVALATAATVIASQAVISGAFSITRQAVQLGFLPRMTIVHTSAREI
                     GQIYIPSVNWVLCVVVIGAVVGFGSSSELAGAYGVAVTGTMLVDTLLTFFVIRYAWRY
                     PLWLCLFATGFFLVIDLAFFSATLLKINDGGWFPLVIASGVFLVMTTWRRGRERLLAH
                     LQESSVPLEPFLKSLFAEPPHRVTGTAVYLTATPDVTPHALLHSLKHYRTLHERVVFL
                     TVEFRDVPWVPFEERVLCERLRNNCWRVGVRYGFMNRLDLLRALELCGALGLEFDMME
                     TSFFLSREKIVPMASWRDRLFAAMARNAGNITDYLNIPTNCVVELGTRVEI"
     gene            11054..12094
                     /locus_tag="VIG70_02680"
     CDS             11054..12094
                     /locus_tag="VIG70_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY8249483.1"
                     /translation="MKWPGYAWAAGATVASTLVALGLDAYFHVVNVALVYVLAVVLVA
                     LRFSRGPMVAVSVLNVVAFNFFFIPPRGTFHVDDPQYLLTFAMMLGVGLIVSGLTERV
                     RARAQAQAALEVEAETERVRSALLASISHDLRTPLAVITGSSSTLAERGERLGAEERA
                     ALAQSIFQQARDMSELVTKVLQMTRLESGAIKLERGWDSLPEIAGAVLRRLHDRLATH
                     LMMVDLPDDLPLVRVDASLIEQVLANLLENAARHTPPRTLIRLRAQVAGGEMVVSVED
                     FGPGLPESDLERIFKKFHKGAGLGLAICRAIVELHKGRIWAERLPGVGTAFRFTLPLE
                     TPPPMPVEAFAR"
     gene            12091..>12359
                     /locus_tag="VIG70_02685"
     CDS             12091..>12359
                     /locus_tag="VIG70_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8249484.1"
                     /translation="MNPTILVVEDEPEIRQFLRTSLKAEGYRVVESPNGRRGSLDAGM
                     HKPDLAIVDLGLPDFDGIEVIRRIREWSPMPIIVLSARIQERSKI"
BASE COUNT         1853 a         4183 c         4254 g         2069 t
ORIGIN      
        1 cgaccgcgcg cctggagctc gctcgggcgt gcgcccggct tcatctcgga gaccagcgct
       61 tcggcgacct gcgcggtggc gcgcaggttg cggcggccct ccgcttgcgg ctcgccgccg
      121 accacccagg tcttgccgcc gtcccagcag tagaggtcga gcgtaccgtg gcagtcgaac
      181 atcacgtgca tgccggcctc cacctcgaag tcctcgagcc cggtctgcag cgtcagtgcg
      241 gcttccgcgc cgcgaggatg gccccacacc attgcgcccg gatcgcgcac gaagccgccg
      301 aggtcgatgg ccgccaaatg gtaggcgcgg ttcagttcgc gccagcgcat gcccttgcgc
      361 cacgacttga ccgtgcgctc gatcgcggct cggttgagcg cggtggcgcg acgcaggagt
      421 tcgagctcgg tgggcgtctt cacggcgcgg gcgtacatga gcacgtcgta cgcctcgccg
      481 ccatttgcgt cctcagcgac cttgccgtgc tcaaggccga ggtcggcgag agcgccgcgc
      541 agcgcggatg ccttgtcgaa cgcatagcgg ctgcgagccg cgcgcaccca ctccaactgc
      601 tttgcttctt gagggatgaa ccgctcgatg tcctcgcgct gcggcgcccg gtcgatcggc
      661 atcatcaccg cgcggaacgg tcgtatgtcg cgcacccagc tcggctgggc gagaaaggtc
      721 gcgaggtaat agtcggcgat cacgaggatc gggtgctcgg gcgcatggcg cgacagcacg
      781 agcgcgtagg gacgcggctc gtccgccttg tgcgcgatgc cgttgaagcc gctgaggtaa
      841 aagacgttga gcggcgtgga ggccacgatg ccgtccagcc cgcgcgctgc catcccggcc
      901 agcaggcgct cgaagttgca gaagcgctcg gggagcggtt ttctatcgag tatcactgca
      961 acgcccgctt cagccgggca acgccgtccg ccagcttctc ttccgaggcg gcgaagcacc
     1021 agcgcacgaa gccttcgcct tccgggccga acgccgagcc gggcgcgagg ccgaggccgg
     1081 tttcgcgcac cagcttcttg cagaacgcga gactgtccgt gactccgtcc accttgaaga
     1141 acgtgtacat cgtcccggtc ggcaccgcga cttcgatttg cgggatttcc ttcagctccg
     1201 aaaccaggaa atctctggcc ttcctgaacc gatagcgcgt gcgcgcgatc accggttcgc
     1261 cctcctttac cgccgcgatg ccggcgcgct gtacgaagac cggcgcgcag gaggtgttgt
     1321 actcgatgag cttgccgaga tccgcggtga ggccgggcgg caccaccagc catcccagcc
     1381 gccagccggt catcagccag gacttggaga acgtgttggc gctgatgagg cgatcttccg
     1441 tttcactgat gttgagaaga gaaggggcaa cgccttcgtc accgtagtac aggcgctcgt
     1501 aggcatcgtc ggcgaagatc cagatcccgt ggcgccggca atgctccagg atcgccttca
     1561 gctcggcggc gctcaccgtc cagccggtcg ggttgttcgg cgagttgagg tacagggcgc
     1621 gggttttcgc cgtgagtgcg ccgaggagac ggtccaggtc cagcttccag cccgaaggcg
     1681 aaaactgcag tgcgaccgtc tccacggtcg cgcccaggat cttggggatc tcctggaggt
     1741 tgggccacag cggcaccacc tgcaccacgc ggtcgcccgg gtcgacgagg agctgcgagg
     1801 cgaccatcag cgcgttcacg cccgcgctcg ttacagcgat ctgctgaaaa tcgaccggcc
     1861 tgtgcagctt cgaaacatac gcggcgagcg cttcgcgcag ctccgggatg ccgaggttgt
     1921 gggtatagaa cagctcgccc gcgccgatcg aatcggtgcc cgccttgcgg atgaagtcgg
     1981 gcgtcggctc gtcgggctcg ccgacccaga acggcagcac gttcgggttg ccgaggcctt
     2041 cgttgtagag ttcgcggatc ttcgaggcgg tcagcgcgcg aaccgcggcg cgcgccgtca
     2101 acggggattc ggatttcatg agccgcccca ttttagactg gtctctcata gcagccgtct
     2161 gcacgatggt ggcgtggggc atgaatttcg ccttcgtcaa gtacgtgctc gactacctcg
     2221 gggtcggcgc gttcatgttc ctgcgtttca ctatcctgcc gctgctcggg ctggtgctgc
     2281 tggtgctcgt gttccggcac cggatccggc acacctggcc gcggcgcgcc gacctgccgc
     2341 gcttcgtcct gtgcgcgctc atcggccacg tgctccacat cagcgccgtg atgtacggca
     2401 tgaacctgtc gaccgcgttc tcgtcgtcgc tcgtgctgac ctgccagccg ctcttcacgc
     2461 tcgccatcct tgcctggctc ggcaccgagc gcctgcgcag gcagcagatc gcaggcaccc
     2521 tcatcgcgtt cgcggggatc gcgctcttcc tcgccgacaa gttcgccgcc ggcttctcac
     2581 gcgccggcct gggcgacctg acgctcctca tcgcgggcgc cgcgttctcc gcctacacgg
     2641 tgctgtcgcg acccctggtc gaccgctacg gcccgctgat cgtgctctcg tacacgctgc
     2701 tgttctcgat gccggtgatg ctcgcggtca cctggccgtt cttcgccgag ctgaaggccg
     2761 tgcacctcgg cccgatggtc tgggcggcga ttttctgggc gctggtggtg tgctccttct
     2821 gcggctggct ggtgtggacc tgggtcaaca gcgtgcgcgg cgtcgcgcgc agcgcgcctt
     2881 tcatgtacct catgccgccg gtggcggggg tgatggcgtg gctcacgctc ggtgagacgt
     2941 ttacctggct gaaaatcgcc ggcgccgcga tcaccatggc aggcgtcgcc tgggcgcagt
     3001 tcggcggccg gccaccgccc aaggaagccg gacaacccga ctccggatga agatcccgcg
     3061 cacggtctgg gcgctcggcg cggtgtcgct gctgatggac ctgtcgtccg agctggtgca
     3121 cagcctgctg ccgctcttca tggtcacggt gctcggcgcg agcatgctga cggtgggcgt
     3181 ggtcgagggc atcgccgagg cgaccgcgtc gatcgtgaag ctgttctccg gcgcgatctc
     3241 ggatcgcttc gggcgcagga agccgatcat ggtaatcggc tacggcctcg ccgcgatttc
     3301 caagccgctc tttccgctcg ccgcgtcggt gccgctggtg ctcgccgcgc gcttcatgga
     3361 tcggatcggc aaggggatcc gaggcgcccc gcgtgatgcg ctgatcgccg acgtcacgcc
     3421 cgaaggcatg cgcggcgccg cctacggcct gcgtcaggcg ctcgacaccg tgggcgcggt
     3481 gctcggcccg ctcgccgcga tcgggctcat gcttctcctc gcgagcgacg tgcgcagtgt
     3541 gctgtggtgg gcgatcgtcc ccgcgtttct gtcggtggtc gtactgatcg ttttcgtgaa
     3601 agaacccgaa ggcgcggtac ggccgctcga gcgattttcc ttgcaatcct tcaatcgact
     3661 tggagttcag acatgggcga tcgtggccct cggtgcggtg cttacgctgg cgcggttctc
     3721 ggaagcgttc ctcgtgctgc gcgcccagga cctcggggtc gcgctcgccc tcgtgccgat
     3781 cgtgctcgtg gcaatgaacg tcgcgtacac cgcggtctcc tacccggcgg gcctcgccgc
     3841 cgaccacggg catcgcaagg cgctgctcct ttggggattg ctcgcgctga ttgcggccga
     3901 cctggtgatc gccgcgacgg cgtcggtgcc tctacttttc ctgggaatcg tgctgtgggg
     3961 cgtgcacatg ggcctgacgc aaggcctgct ctccacgctg gtcgccgccg ccgcccccca
     4021 caatctgcgc ggcacggcgt tcggcgtgtt ccacctcgcg tccggggtcg cgatgctgct
     4081 cgcaagcgtg atcgccggcg ccctgtggga tggctatggc gccgcggcga cgttctatgc
     4141 cggtgctggt tttacggggt tcgcactcct tggccttctc ctagttcgcc cccagacagc
     4201 gacgaaatag ctcggcggcg gtccgcacgc caagcttctg catgatgcgc gcgcggtgga
     4261 actccaccgt cttcaggctg atcgagagct cttcggcgat ctggcggttg ggcttgccct
     4321 cgagcacgcg ctcgagcacc gcgcgctggc gcgcgctcag cgctgcgagc ggcgctttct
     4381 ccggctcgtg cctttcggtc tcttcatcgt agtcctcgag cgcgcccagc acctgcccga
     4441 gcaggtggtc gtcgtcgaac ggcttctcga tgaagtcgta ggcgcccttc ttcatcgcct
     4501 ccaccgccat cgggatgtcg ccgtggccgg tgatgaagat caccggcgcg cgcacgccgc
     4561 gcccgaggag ctcctcgtgg agatcggtcc cggtcatccc gggcatgcgg atgtcgagca
     4621 gcacgcagca gcgccgctcg aggttcgccc gctccaggaa cgcggaggcc gaggaaaagg
     4681 tttccacctg caggtcggcg gtgcggaaaa gcagcgacac ggcgttgcgg accgcatcgt
     4741 cgtcgtccac cacgtacacc acgcgcccgg gcatcatctc ggcagcgtga acgcgaagcg
     4801 cgcgccgcgc ccgttgtggt ccaggcgaat ctgtccgccg tgagcctcga ccacggaacg
     4861 gcagatggag agtccgaggc ccaggccgcc gcgcttggtg gtgacgaagg cgtcgaacag
     4921 gcgctcacgc acctccggca gcaccccctc tccgaggtcg gacaccgcca gcgtgccgct
     4981 gccgtcgagc tcgacgcgcg tggcgaccgt gatgcggcgg cgctccggcg cgagctcctg
     5041 caccgcctcg acggcattgc ggaccagatt gagcgctgcc tgctcgatca gcaggcggtc
     5101 ggcgcgcacc accggcggtc gcgccgacag cgcgcgctcg aacgacacgc cggcatcgcg
     5161 cgccccgggc tcggcgagct cgagcaccgc ggcgaccagc tcgttcaggt cctgctcggc
     5221 gagcgccggg cccgggtggc gcagcaggcc gcgcaggcgc tggatgatgc gtccggcgcg
     5281 ctttgcctgg tcgcggcaga tgtgcatcgc ctgcaggagc tgctcggggt ccgggttgcc
     5341 cgactgcagc atgcgcatgc agctatggtt gtaggtggcg atcgcggcga gcggctggtt
     5401 cagctcgtgc gccaccgccg aggcgaactc gccgagcgcg atcaggcgcg cggtgcggtg
     5461 cgcggcgtcg cggtgcttgg cgatcagctc ccgcgcgcgg cgttcctcgg tgacatcgga
     5521 cagaatgcaa agcgacgcct tgcgcccgtt gatccaagag agcggatgcg cgcgcagcac
     5581 gtaccagcgg tcgttggccg catcgtgaaa ctcgccctcc gcctcgagct caggcggcgt
     5641 tgtaaaaatt tcaaggtagc ggcgattgcc gtagaggaac gagccgccgt cgatctcctg
     5701 cacatagacc ggaaatggca gcgcctcgac cagggccgag agcagcgcca tggcggtctc
     5761 gtcgatggca agcatgagaa cgagcttact agggtttgcc ccagttgttg ccgcgcgggc
     5821 ggttgtttag cctgcacttc agggtgggag atcctgacaa caggctgagc agagcgctcg
     5881 agggattcga cctgctgctc gccgccgcca ccgcgctggt gcgcgcaaaa tccgcccggc
     5941 ccgcgctgct cgacgcctac gacgacgctt cggatcgcct gatggaaggc ctgcgcggca
     6001 acggcgtccc cgggcacgag ctgcaggcgg tgcacaaggc gctcgcgcgc ctgcgcctcg
     6061 ctttcgaaca gacgccccct agctgaagaa gccgcggaac gccgcgagcg cgcggtcgat
     6121 tcccgcggcg tcgacatcga gatgcgtcac cagccgcatc ggcgcgcgcg gcagcacgag
     6181 gatgccgctc ttcttcatgt gcgcttccag cggctgcgct ttttccggcg gaatcttcac
     6241 cagcaccatg ttggtgttgt gctggacctc gagcttgagc tcgcgcagcc cgcgcgcgag
     6301 cttctcggcg ttcgcgtgat cgtccgccag gcgctcgacg ttgttctcca gcgcgtagag
     6361 cccggcggcg gcgatcacgc ccgcctgccg catgccgccg ccgaggattt ttctggctct
     6421 cttggctttc tcgatgagag gcttgtttcc cagcagcact gtgcccgcgg gcgcgccgag
     6481 gccctttgac aggcaggagg aaacggagtc gaagccggcg cacaggtcct tcaccttcat
     6541 cctgagctgc gtggccgcgt tgaagacgcg cgcgccgtcg aggtggactg aaagattttt
     6601 ccgttttgcc agctcgatcg cgtcctgcag gtattttcgc ggcaacaccc ggccggtgat
     6661 cgtgttctcg agcgccagca ggcgcgttct ggcgaaatgc gggtcgtcgg gcttgattgc
     6721 ggcttccacc tcggcgaggt cgagcgtgcc atcggggcgg ttcgggatcg cctgcggctg
     6781 gatcgagccg agcacggaga ggccgcccgc ctcgtagcgg tagctgtgcg cttccatacc
     6841 gaggatcacc tcgtcgccgc gctggcaatg gctcatcagc gcggcgaggt tggattgcgt
     6901 tcccgacggg aagagcagcg ccgattcgaa gccgaagatc tccgcggcgc gcgcctgcag
     6961 ctggttgacg gtagggtcgt cgccgaagac gtcgtcgcca agctcggcgt cgttcatcgc
     7021 cttgcgcatt cccggcgacg ggcgcgtgac ggtatcggag cgcaggtcga tcactggttg
     7081 aaccccagtc gttgcgacag cttcgcgctc agctcgccga gcggcgcgcg caggtcgcgc
     7141 aggcgctggt aggtcatgcg gctcttcggc ccgagggtgc agatggagcc gagcacctgg
     7201 ccggtgtggc cgaacaccgg catggcgatg cagcggatgc cctcgatgtg ctcctcgtcg
     7261 tcgacggcgt agcccgcgga gcggatgcgg cgcagctcgg tctcgaacgc ggcgcgcgtg
     7321 acgatggtct tcggcgtgtg gcgctcgaag cggatgcgcg cgaggagccc ggcaagctcg
     7381 ggcgcggcga gccacggcag gatcgctttc gcgaccgcgg tgcaatggag cggccgccgc
     7441 gcgccgacgt cctgcaccac gcgcaccggg ccgtcgtgct cgcgcttcgc gactaccgtg
     7501 acggcgccgt cgcggtacac ggagaagctc gagccctcgc cgctgcgccg cgtgagctcg
     7561 gcgagcgagg tctgggcgat ccccgcgagc tgctccggcg accaggtgcg cccggtgatc
     7621 tggaacggct tggccgcgag ctcgtaggcg cgcgtgtcgg cgtcctggcg caggtagccg
     7681 aggctcacca gcgtctgcag aaggtggtgc gcggtgctgc ggtgcagccc ggtgcgccgc
     7741 gcggcttcgg cgagcgtggt ctggacgccg ttcagcgcca ccgcttcgac caggcgcagg
     7801 ccgcgctcga gggactggtg ggtgcgttgt ttcatacgct gagaacgttg tcgcgagggc
     7861 gtttcagggg ccagaatacc cgctcctctg gaggaggcca acatgaaggg gtggtggatg
     7921 ctcacgcggg cggccgcgcg ggccgtaccg gcccatgccc agcccaagca caccctgaag
     7981 ttcaaccacg gcaagaagta ggttaccccc gagggacgga tcgccagaaa atagggtccg
     8041 tccctatttt ttcctctatt ttttcctctt gagagccctt tacaagatcg ccgcgccgct
     8101 aggatgcgcg cgtgctcatc aactgcgtgg cctaccagga aggccgcagg ctcgccgaca
     8161 tcgagctgcg cgagatccac gcccacttag cgcagcccgg tacgttcgtg tgggtggggc
     8221 tgcgcgaccc gacgcaggcc gagctcgcca cgatgcagga gcagttcgac ctgcacgacc
     8281 tcgcggtcga ggatgcgggc cacggccacc ccgccgtagc ccgccccaag gtcgaggagt
     8341 acggcgatgc gctgttcgtg gtgatgaacg ccgtagagct atcgcccacc gacgagatca
     8401 agctgggcga ggtcggcgtg ttcgtcgggc ggaactatgt gctgtcggtg cgcaggcgca
     8461 gcgaccgcgg cttccaggaa gtgcgcgcgc gtgcggagcg cgagcccgat ctcctgcgcc
     8521 acggcccggg ttacgtgctc tacgccctga tggacgccgt ggtcgaccgt tacttcccgc
     8581 tgctcgacgc gatcgaggtg gagctcgaag ccatcgagga gcgcctgttt acgcccggcg
     8641 cctcgccgcg cgagaacatc gagtcgctct attacgtgaa gcagcggctc accaccatca
     8701 agcacgccgc cggcccgctg ctcgagagca cggcgcggct gttcggcggc cgcgtgccgc
     8761 ccgtctgcgc cggcctcggc gaatacttcc gcgacgtcca cgaccacctg gtgcgcatca
     8821 accagtcgat cgacgccgcg cgcgagaccg tcggcaccgc gatccaggtg gcgctcgcca
     8881 tggtcgccac cgggcagggc gagatcgtgc gccggctcgc cgcctacgcc gcgctggtgg
     8941 ccgtgccgac gatgatcgtc ggggtctacg gcatgaactt cgcgcacatg cctgagctga
     9001 agtggacgtt cggctatccg ctggtgctgg gtgccatcgc cgccatcgac gcgttcgtct
     9061 tctatcgctt ccgcaaggcc ggctggcttt agcagggagc cggccgctag atctccacgc
     9121 gcgttcccag ctcgaccacg cagttggtcg ggatgttgag gtagtcggtg atgttgccgg
     9181 cgttgcgcgc catcgctgcg aaaaggcgat cgcgccagga cgccatgggc acgatcttct
     9241 cgcgcgagag aaagaacgag gtttccatca tgtcgaattc gaggccgagg gctccgcaga
     9301 gctcgagcgc gcgcagcagg tcgaggcggt tcatgaagcc gtagcgcacg cccacgcgcc
     9361 agcaattgtt cctcaggcgc tcgcacagca cccgctcctc gaacggcacc catggcacgt
     9421 cgcggaactc gaccgtgagg aacaccacgc gctcgtgcag ggtgcggtag tgcttgaggc
     9481 tgtgtagcag cgcgtgcggc gtgacgtccg gcgtcgcggt caggtatacc gcggtgcccg
     9541 tcacgcggtg aggcggctcg gcaaacaggc tcttgaggaa gggctcgagc ggcaccgacg
     9601 actcctgcag gtgtgcgagg agccgctcgc ggccgcggcg ccaggtggtc atgaccagga
     9661 acacgccgct ggcgatgacg agcgggaacc agccgccgtc gttgatcttg agcagcgtgg
     9721 cggagaagaa cgcgaggtcg atgacgagga aaaatccggt cgcgaacagg cacagccaca
     9781 gcggatagcg ccaggcgtag cggatgacga agaaggttag cagcgtgtcg accagcatcg
     9841 tgccggtgac cgcgacgccg tacgcgccgg cgagctcgga cgacgagcca aacccgacca
     9901 ccgcgccgat caccaccacg cacaggaccc agttcacgct cgggatatag atctggccga
     9961 tctcgcgcgc cgaggtgtgc acgatcgtca tccgcggcag gaagccgagc tggaccgcct
    10021 ggcgcgtgat cgaaaaggct ccgctgatca ccgcctggga ggcgatcacc gtggccgcgg
    10081 tggcgagcgc caccatcggg taaagcgccc atccgggcag gagcaggtag aacgggttgg
    10141 acaccgccgc tggctcggtc atcagcagcg cgccctggcc aaaatagttg agcaccagcg
    10201 cgggcgcgac caggccgaac caggcgatgc gcaccggccg ccggccgaag tggcccatgt
    10261 cggcgtagag cgcctccgcg ccggtcacca ccagcaccac ggcgccgagc accacgaacg
    10321 actcgaccgc gtgcgtcatc acgaagcgca gggcgtgcag cgggttcagc gcggcaagca
    10381 ccgccggctc gcgggcgatg ccgtgaattc cggcggcagc gatcgccaga aaccagagca
    10441 gggtcaccgg cccgaacagc gtgccgaccg cctcgctgcc gcgggactgg agcgcgaaca
    10501 gcgccgccag cacgccgacc gcgatgggca cgacgaaggg cttgagcgcc ccggtgccca
    10561 cctcgaggcc ctccactgcc gacagcaccg agatcgccgg cgtcaggacc gcgtcgccgt
    10621 agaagagcga tgccccgaac acgccgatga tgagaagcgg caggcgccag tgcggctggg
    10681 cccggatcgc ctgcgtggcg agcgcgatca gcgccatgat gccgccctcg ccgcggttgt
    10741 cggcgcgcat gatgagcatc acgtacttga gcgagacgac ggtcatcagc gaccagaaca
    10801 tcagcgacag gccggcgagg atgttctcgt gcgtgagcgc gatgccgtgc tcggcggaga
    10861 aggtctcctt caccgcgtag agcgggctgg tgccgatgtc gccgaacacc acgcccagcg
    10921 cgatgagggt cagggcgggc gtcgattggc gcgcgggcat cgggagcgaa gcctacggca
    10981 gcgggcggca ccgtgatcgt gtcgcggtca gtttttacgg cgtcttaacg cgggcgtttg
    11041 ctagagtgag cgcatgaagt ggcctggata cgcgtgggcc gcgggcgcca ccgtcgcatc
    11101 gacgctggtg gcgctcggcc tcgacgcgta cttccatgtc gtgaacgtgg cgctggtcta
    11161 cgtgctcgcc gtggtgctgg tggcgctgcg cttcagccgc ggcccgatgg tcgcggtctc
    11221 ggtgctcaac gtggtcgcgt tcaacttctt cttcatcccg ccgcgcggta ccttccacgt
    11281 cgacgacccg cagtacctgc tgaccttcgc catgatgctc ggcgttggcc tgatcgtctc
    11341 gggcctcacc gagcgcgtgc gcgcacgggc gcaggcgcag gccgcgctcg aggtcgaggc
    11401 cgagaccgag cgcgtgcgca gcgccctgct cgcctcgatc tcgcacgacc tgcgcacgcc
    11461 gctcgcagtg atcaccggat cctcgtcgac gctggcggag cgcggcgagc gcctcggcgc
    11521 cgaggaacgc gccgcgctcg cgcagagcat cttccagcag gcgcgcgaca tgtcggagct
    11581 cgttaccaag gtgctgcaga tgacgcgcct ggagagcggc gcgatcaagc tcgagcgcgg
    11641 ctgggactcg ctgccggaga tcgccggcgc ggtactgcgc cgcctgcacg accgcctggc
    11701 aacgcatctg atgatggtcg acttgcccga cgacctgccg ctggtgcgcg tcgacgccag
    11761 cctgatcgag caggtgctgg cgaacctgct cgagaatgct gcgcgccaca cgccgccgcg
    11821 taccctcatc cgcctgcgcg cccaggtcgc cggcggcgag atggtggtct cggtggaaga
    11881 cttcggcccc ggcctgccgg agagcgacct cgagcgcatc ttcaagaagt tccacaaagg
    11941 cgccggcctc ggccttgcga tctgccgcgc catcgtcgag ctgcataagg gccgcatctg
    12001 ggccgagcgc ctgcccggcg tcggcaccgc gttccgcttc acgcttcccc tcgagacgcc
    12061 gccgcccatg ccggtcgaag cgttcgcgag atgaatccca ccattctcgt cgtggaggac
    12121 gaacccgaga tccggcagtt cctgcgcacc tcgctgaaag ccgagggcta ccgcgtggtc
    12181 gagtcgccga acggtcggcg cggctcgctc gacgccggca tgcacaagcc cgacctcgcg
    12241 atcgtcgacc tggggctgcc cgacttcgac ggcatcgagg tgatccgccg catccgcgaa
    12301 tggtcgccga tgccgatcat cgtgctgtcg gcgcgcatcc aggagcgctc caagatcga
//
LOCUS       DATBSZ010000040        14094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_824646, whole genome shotgun sequence.
ACCESSION   DATBSZ010000040 DATBSZ010000000
VERSION     DATBSZ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14094
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824646"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1691)
                     /locus_tag="VIG70_02690"
     CDS             complement(60..1691)
                     /locus_tag="VIG70_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8249485.1"
                     /translation="MLDLLFRGASIVDGSGKPAHQADVGVSQGRIAEVGAIRSSSRQT
                     VDCAGLSLMPGIVDVHTHYDAQVTWDPTLSPSVSLGVTTAIMGNCGFGIAPCPAPLRE
                     TVLRNLSVVEGMDLEALLAGTRWQFETFGEYLDALERIGPYANVGVLAQHSTIRTAVM
                     GEEASVRKSPSSEQLERMKSQVREAMRAGAAGFASSFSPNHSGWGGRPMPSTIASDEE
                     LAQLVGVLKDEGKGVFVVAAGSRATPDYLEAIAAETGRPAFMVTVLTMHNKAQPGRAM
                     EYYERCAAALARGREVYIHANCQPLSFDFTLRDPYILYSHDAFDRVKAAAPAERAAIY
                     QEKTFRDRLRENLRNPKPGILFYGDWSQVEKDGMPVTQLAADPLDYVFDLPLGTQLVA
                     KLFQNDDDAVAPLLRHPAGVIALSDAGAHLIYFCDAGFGLHFLAHWVRENRAFTLEEG
                     VRRLTSDPARKYRIPRRGLIARGYAADLLLFDPAAVGVGNLERVADLPGGGTRMIRKP
                     RGVHGVWVNGLQAFDGAKYIRRQCGSGEVIRNFDP"
     gene            1739..2692
                     /locus_tag="VIG70_02695"
     CDS             1739..2692
                     /locus_tag="VIG70_02695"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEY8249486.1"
                     /translation="MKKIFVVGFMFTASQVFAQAGFPPPPPKVSPATGVIDMHVHSHP
                     DVFGRNMDDIDVAQLAKSKGMRGILLKNHVAETASRAALVMKVVPGIEVFGGVVLNKA
                     VGGINPDAVEWMHRVYGGRGKVVWLPTFEADRHVKVLSKPDARGLVVAPGGQVTPEME
                     AILKIVARENLVLATGHVHPEEIIAVVRRGRELGVKSMLITHGLTNVPGLTMAQAKQV
                     AEMGAVIEVCFLQFLAGPNAPLAFLTHWTQINAKHVAQALKEIGAKSLVISSDLGQSG
                     NMTHPDGIEAAIAAMKREGISDGDIDVMMRKNPARLLGLAD"
     gene            2664..2867
                     /locus_tag="VIG70_02700"
     CDS             2664..2867
                     /locus_tag="VIG70_02700"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8249487.1"
                     /translation="MRVCWDWRTEVFRPSPDVSRAGHAGVALRGMVDSARLIREAEDA
                     ALGVAGVAAVDNHLISADLFEHD"
     gene            complement(2938..3474)
                     /gene="pal"
                     /locus_tag="VIG70_02705"
     CDS             complement(2938..3474)
                     /gene="pal"
                     /locus_tag="VIG70_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY8249488.1"
                     /translation="MKLASIAMLAVVAGLYGCATTESQDSSSSTPGATPSASASTSGG
                     VTPGTAQPPARMGTGFTGTKPGTRPDLKYSVYFEYDQYDVKPEYRTLVESHARWLKAN
                     PQARLVIEGNADERGSREYNVALGQRRAESVTKLLVLLGAKPEQIEAVSWGEEKPRVT
                     GHDEKSWGENRRDDFVQR"
     gene            complement(3546..3983)
                     /locus_tag="VIG70_02710"
     CDS             complement(3546..3983)
                     /locus_tag="VIG70_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8249489.1"
                     /translation="MRFLLMLLFAGVGCALAEPGEPEEGGSDPEYTQGVAAIEAKDWK
                     TAIRLLSSAALRDTRNAEIQNYLGYAYRNAGELPLAFKHYSRALQLNPRHRGAHEYLG
                     EAYLMIGNLPKAQEHLEILRKICLIPCAETGHLEGKIAAYRAR"
     gene            complement(3980..5575)
                     /locus_tag="VIG70_02715"
     CDS             complement(3980..5575)
                     /locus_tag="VIG70_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249490.1"
                     /translation="MKTLIALLFAFTATGALAHDKVGSEKLGKVLFKTSCTPQAQKEF
                     ERALGMLHSFWFPETVKVFSAIPQTDPGCAIAYWGLAVSIRPNPLVGPWDAATLKRGL
                     EAVEKGEAIGAKTQRERDWLAAIKEFYKNYEAVDQDTRTANYEKAMAALVKKYPNDVE
                     AKVFHALALNEVFDHKNMAPLQQAIKILEPLDKKYPDHPGIAHYLIHSYDFAPLARKG
                     IPAANKYAKIAPSAPHAQHMPSHIYTMVGMWKDSIEANLKSNVVSREYAAEAKLDGVL
                     VGVPHANDFMQYAHLQLGQDGKARQLIEETAQIKKVLGPIHAGQMARAAVPARYFLER
                     QDWKGAAALQPLGSPFPAAEAITHYARAMGAARSGDAAAAQADIEKLKGLREQIEKAK
                     QAYWAEQVEIQILSAQAWLANAQGNKDEALKFMRAAADLEDSSEKHVAMENRLYPMRE
                     QLADMLREHGQPGAALKEYEASMKAAPNRLRGYYGAAKAAEAAGAPKKAAGYYQNLAR
                     LTKEADSDRAELREVKEWLAARQ"
     gene            5689..7329
                     /locus_tag="VIG70_02720"
     CDS             5689..7329
                     /locus_tag="VIG70_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEY8249491.1"
                     /translation="MDRAPSSSNFIRQIVDADLAAGTYAGVRTRFPPEPNGYLHFGHV
                     KSICLNFGLAEQYGGRCNLRFDDTNPEKEEQEYVDSIKEGVKWLGFDWGQTDLYASDY
                     FGKLYQFAEALIEAGQAYVDSQSAEAMSAARGTFTEPGKDSPWRSRSVDENLALFRGM
                     RDGKYKDGAHALRAKIDMRSPNLNLRDPVMYRIRHAHHHRTGDKWCIYPTYDWAHGLS
                     DCLEQITHSLCTLEFEDHRPLYDWFNERVAEAGLIQKPLSKQIEFARLNLTYVVLSKR
                     RLIQLVEQKIVDGWDDPRMPTLAGARRRGYTPEGFRMFAERIGVSKAHQVIEFGILEE
                     SMREHLNDVAPRRMAVLDPVKLVIDDYPEGKEELMDLPSHPQKPEWGKRQVPFSRELW
                     IEREDFQEKPVKGYFRLFPGNEVRLRYGYVVKCIGMKDGVVHCTHHPDSRSGSPGADK
                     YKVKGNIHWVSAKHAHAVKVRLYDRLFRVAEPGKDRDFLQDLNPDSRKTVDAQVEPSL
                     RDRSKRQAYQFERHGYFIADYDGVTFNRTVTLRDSWKA"
     gene            complement(7403..8710)
                     /locus_tag="VIG70_02725"
     CDS             complement(7403..8710)
                     /locus_tag="VIG70_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249492.1"
                     /translation="MKLRPAGLAFAALASVIGVVFGQQQLSENDKQISAHAQRLLDEG
                     RRIFRFDTMGSESFWGDALQLHRAIAGAKNGGVGGGLSPKAALSVGLKVDADALPSEL
                     VAQIKAGKVDLEDPATTLALLKVNAVVGVTGIFDNGRLRSMGVQCSLCHSTVDDSFAP
                     GIGKRLDGWANRDLNVGAIVSLAPNLKPFTELLKVDEATVKKVLASWGPGRYDALLDK
                     DGKAFRPDGGQAGTLIPPAFGLAGVSLNTWTGFGSVPYWNAYVGTTQMHGNGTFIDKR
                     LSDKKQYPVAAKSRSAETRKAPDQVTPKLAALHFYQSSIPAPRPAAGTFDAAGAKRGE
                     ALFNAKAKCSSCHVPPLFTEPGHSLHSPKEMGIDAFQADRSPTRMYRTAPLAGLWTHQ
                     KGGFYHDGRFATLAEVLEHYDRHFKLQLTDPEKVDLMEYLKSL"
     gene            complement(8852..9004)
                     /locus_tag="VIG70_02730"
     CDS             complement(8852..9004)
                     /locus_tag="VIG70_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249493.1"
                     /translation="MKPPCVGCFSRVDLRKGTKEPATLCTRDVFVPCPDERGQGCTPK
                     ETYRCN"
     gene            9014..9499
                     /locus_tag="VIG70_02735"
     CDS             9014..9499
                     /locus_tag="VIG70_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8249494.1"
                     /translation="MSKPDPKWKLDGVRVVHSNELDINTPQTPGMNRAAAITRARAGA
                     EKLWAGTVVIHPKAKTGAHHHGAVESVIYVVSGKARMRWGEKLEFVAEAGPGDFIYVP
                     PYVPHQEINASDTEPLSCVLCRSGQEPVVVNLPDLPVVEKPEEVHWVDNIHPDPTKKP
                     V"
     gene            9615..9818
                     /locus_tag="VIG70_02740"
     CDS             9615..9818
                     /locus_tag="VIG70_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY8249495.1"
                     /translation="MSTGTVKWFNDAKGFGFITPDDGSKDLFVHFSAIQASGFKTLKE
                     NDKVQYEVEQSPKGPRATNIKQV"
     gene            complement(9870..11255)
                     /locus_tag="VIG70_02745"
     CDS             complement(9870..11255)
                     /locus_tag="VIG70_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8249496.1"
                     /translation="MGADPKTTVSRAEFLNLFAAVFLPMFMAAVDQTLLATATPAIAG
                     SLGDLRDSSWIVVGYLLASATIVPLYGRLGDRHGRRNVLLAALGVFALGSAACGLAQT
                     LPQLVAARVLQGLGGGGLMTLSQALIGELVPPRERIRFQGYFALVFTTASIGGPVLGG
                     LVVSHTSWRWLFFANLPFAAFAAWRLSRLPAGERHPEAASGRDVPGQLLFAVAAVSTL
                     YWLTSVGHRFAWGSTWSLALAALALVSIAALAWHESRQAAPFLPVDLLRNRTVGLSAT
                     LTALFAASLFAVIFFLPIYLQLGHRVSPLLSGLLLLPVTAGMVTAALAASKLLGRTGE
                     AHWIPVGGMSLAAVALLLLGLLPDDQGLVIALGFLTGLGLGVVMPINQVVVQTVAGRA
                     RLGAAMALMGLARSTGGATGAALFGAVVFALMPHVDVVEAFHRAFLVAAGVAVLGALT
                     ASRIPRVKLWP"
     gene            complement(11259..12086)
                     /locus_tag="VIG70_02750"
     CDS             complement(11259..12086)
                     /locus_tag="VIG70_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY8249497.1"
                     /translation="MIIEPLAGALGAEIRGVDLKDDGTWEAIHDAFLKYSVLAIRDQQ
                     LEPADLMRIGARFGEPCYYPFVTGIEGFPFIFEVVKEESETVNFGGNWHSDTTYLPQP
                     PLATLLYALETPARGGDTLFTSTYAAYDALSEGMRAMVDQLVGVNSASLKVTGGRRKM
                     HSTIGGMKVQDIEHADGYEAEHPVARTHPDTGRKALYLSRSHTIRFRDMGEEESRPLI
                     DFLQAHQVRPEFTCRVRWSPGTLTVWDNRCTQHSAVNDYHGQRRRMRRLTVGAQTPK"
     gene            12098..13147
                     /locus_tag="VIG70_02755"
     CDS             12098..13147
                     /locus_tag="VIG70_02755"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY8249498.1"
                     /translation="MTKRIAIVGAGALGGYVGGTLAHLGHDVTLIDPWPEHVEIVRKR
                     GLELDGVTPEEKFVARPKILHLTEVQGLAKTPFDVAMVAMKSYDTLWATALIAPYLSE
                     QGFVVSLQNCMNEETIAGVVGWGRTVGAVASLISVDLYEAGRIRRTIAKGGDKYTIFR
                     IGEPHGRITPRIEQLVEWFRGIDSAKATTNLWGERWSKLVQNSMGNGVTAATGMTSPA
                     CLGDAKIRRFQIALAGEAIRVGQALGYTLEKVRAVEPEKFARANEGDAQAMAEVEAAL
                     IPKAGANPRGDIQRPSMAQDILKGRRTEIEAMNGYIARKGAEAGVPAPSHAKLAAIVT
                     RIERGEVKPSPSLLA"
     gene            complement(13119..>14094)
                     /locus_tag="VIG70_02760"
     CDS             complement(13119..>14094)
                     /locus_tag="VIG70_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY8249499.1"
                     /translation="AARLDIDLQADLMRYTVDVTAGLAFGADINTLESDEDVIQTHLD
                     KIFPSLFKRLMAPVPYWEWFRDRKLDRHLEALHRAVGDFISQARSRMEANPKLREQPT
                     NLIEAMIAARDNPGSGLSDTDVAGNVLTMLLAGEDTTANTLAWMIYLLHRHPDALRRA
                     CEEVGSKDLEKYEEVTALPFIDACINETMRLKPVAPIIMLQPTRDTVVTDIEVPKETL
                     IMCLMRAGATDERRFPDAGAFDPGRWLTGASASSPKRVSMPFGAGPRLCPGRYLAILE
                     MKMVMAMLLGGFDIEQVTTPDGREAQEHLAFTMFPVGLRLKLTKTATA"
BASE COUNT         2407 a         4662 c         4645 g         2380 t
ORIGIN      
        1 acccatggcg taaccctatt cggtgcaggt tacgcacgac catggtgcgg cgcgctagat
       61 catgggtcga agttcctaat cacctcgccc gaaccgcact gtcgccgaat atatttcgcg
      121 ccgtcgaagg cctgtagtcc gttgacccag acgccgtgca cgccgcgcgg cttgcggatc
      181 atgcgcgtgc cgccgcccgg gaggtccgcc acgcgctcga ggttgccgac cccgacggcc
      241 gcggggtcga acagcagcag gtccgcggca tagcctcgcg cgatcaggcc gcgccgcgga
      301 attctgtact tgcgtgccgg gtcgctggtc aggcggcgta cgccttcctc gagcgtgaac
      361 gcccggttct cgcgcaccca gtgcgcgagg aagtgcagcc cgaagccggc gtcgcagaaa
      421 tagatgaggt gcgcgccggc gtccgacagc gcgatcaccc cggccgggtg ccgcagcagc
      481 ggcgccaccg cgtcatcgtc gttctggaac agcttcgcca cgagctgcgt gccgaggggc
      541 agatcgaaga cgtagtcgag cggatcggcg gcgagctgcg tcaccggcat cccatccttc
      601 tccacctggg accagtcgcc gtagaaaaga atgcccggct tcggattgcg caggttttct
      661 ctgagtcgat ctctaaacgt cttttcctgg taaatcgcag cccgttccgc cggcgccgcg
      721 gccttcacgc ggtcgaaggc gtcgtgcgaa tacaggatgt acgggtcgcg cagggtgaag
      781 tcgaacgaga gcggctggca gttcgcgtgg atgtacacct cgcggccgcg agccagcgcg
      841 gccgcgcagc gctcgtagta ctccatcgcc cgcccgggct gcgccttgtt gtgcatggta
      901 agcaccgtca ccatgaacgc cggccgtccg gtctcggcgg cgatcgcctc caggtagtcg
      961 ggcgtggcgc gcgagcccgc cgccacgacg aacacgcctt taccttcgtc ttttaacacc
     1021 cccacgagtt gcgccagctc ttcgtcggat gcgatcgtgg aaggcatcgg gcgaccgccc
     1081 cagccgctgt ggttcggcga gaacgacgaa gcgaatcccg ctgcacctgc gcgcatcgct
     1141 tcgcgtacct ggcttttcat tcgctccagt tgctcagacg acgggctttt tcgtaccgaa
     1201 gcctcctccc ccatcaccgc ggtgcggatc gtggaatgct gcgccagcac tccaacgttt
     1261 gcgtaagggc cgatgcgctc gagcgcgtcg aggtactccc cgaaagtctc gaactgccag
     1321 cgagtgcccg caaggagcgc ctcgaggtcc atgccctcga ccaccgagag attgcgcaac
     1381 accgtctcgc gcagcggcgc cggacagggc gcgatgccga aaccgcagtt gcccatgatc
     1441 gcggtggtga cgccgagcga caccgacggc gacagggtcg gatcccaggt gacctgcgcg
     1501 tcgtagtgcg tgtgcacgtc gacgattccc ggcatcaggg agaggccggc gcagtcgact
     1561 gtttgcctcg aggaggacct gatcgcgcca acttcggcga tacggccctg gcttacgccg
     1621 acgtcagcct gatgcgcggg cttcccgctg ccgtcgacga ttgacgcgcc gcgaaacaaa
     1681 aggtcgagca tttcagcaag ctaccagata tgatttttgg caaccacggg aggagtcaat
     1741 gaaaaaaatc tttgtcgtcg gtttcatgtt caccgcgtcc caggttttcg cgcaggccgg
     1801 gtttcctcct cctccgccca aagtcagccc ggccaccggc gtgatcgaca tgcacgtgca
     1861 ctcgcacccg gatgtcttcg gccgcaacat ggacgacatc gacgtcgcgc agctcgcgaa
     1921 atccaagggc atgcgtggca tcctgctgaa aaaccacgtt gccgagaccg cgagccgggc
     1981 cgcgctggtg atgaaggtgg tgccgggaat cgaggtcttt ggcggcgtcg tcctcaacaa
     2041 ggccgtcggt ggcatcaatc ccgacgcggt cgaatggatg caccgggtct atggcgggcg
     2101 cggcaaggtg gtctggctgc cgaccttcga ggccgaccgg cacgtcaagg tgctctcgaa
     2161 gcccgacgcg cgcggcctgg tggtcgcccc cggcggccag gtgacgccgg aaatggaagc
     2221 gatcctgaag atcgtcgcgc gcgagaacct ggtgctcgcc accggccacg tccatcccga
     2281 ggagatcatt gcggtggtgc ggcgcgggcg cgagctcggc gtgaagagca tgctgatcac
     2341 ccacggcctg accaacgtcc ccggcctcac catggcgcag gcgaagcagg tcgcggaaat
     2401 gggcgcggtg atcgaggtgt gcttcctcca gttcctcgcc gggccgaacg ccccgctcgc
     2461 cttcctcacc cactggacgc agatcaacgc caagcacgtc gcccaggcgc tgaaggagat
     2521 cggcgcgaag agcctggtca tctcgtccga cctcggccag agcggcaaca tgacgcatcc
     2581 cgacggcatc gaggccgcta tcgccgcgat gaagcgcgag ggcatctccg acggcgacat
     2641 cgacgtcatg atgcgcaaga accctgcgcg tctgctggga ctggcggact gaggtttttc
     2701 gcccttctcc tgacgttagc cgcgccggcc acgctggcgt cgctctgcgc ggcatggtgg
     2761 acagcgcccg gctgatccgc gaggccgagg acgccgcgct gggcgtcgca ggcgtcgcgg
     2821 cggtcgacaa tcacctgatc tcggccgacc tcttcgagca cgactgaaaa agaatcgggg
     2881 ccagaccctg cggtctgacc ccgatttttg atcccttttc ttactgcaag aaaatcttta
     2941 ccgctgcacg aaatcgtcgc ggcggttctc cccccacgac ttctcgtcat gcccggtaac
     3001 gcggggcttt tcctcgcccc agctcacggc ctcgatctgc tcgggctttg cgccgagcag
     3061 caccagcagc ttggttaccg actccgcgcg gcgctgtccg agcgccacgt tgtactcgcg
     3121 gctgccgcgc tcgtcggcgt tgccttcgat caccaggcgc gcctgcgggt tggccttcag
     3181 ccagcgcgcg tggctctcga ccagcgtgcg gtactcgggc ttcacgtcgt attggtcgta
     3241 ttcgaaatac acgctgtact tgaggtcggg gcgcgtgccg ggtttggtgc cggtaaagcc
     3301 cgtgcccatg cgtgccgggg gttgtgccgt gccgggtgtg acgccgcccg acgtgctggc
     3361 gctcgcggag ggcgtggcgc cgggggtgct gctgctgctg tcctgcgatt cggtcgtggc
     3421 gcaaccgtaa aggccggcga ccactgcgag cattgcgatt gaagcgagtt tcatgggtat
     3481 cctcccagtt cgagttgtgc gcgaaggata atccaaaaac ccgtgttgcg tcagtgaggt
     3541 atgtctcaac gggcccggta ggcggcgatc ttcccctcaa ggtggccggt ctcggcgcag
     3601 gggatgaggc agatcttcct caggatctcg aggtgctcct gggccttggg gaggttgccg
     3661 atcatgaggt aggcctcccc gaggtattcg tgcgccccgc ggtgccgggg gttgagctgc
     3721 agtgcccgcg agtagtgctt gaacgcgagc ggcagctccc cggcgttgcg gtacgcgtac
     3781 ccgaggtagt tctggatctc ggcgttgcgc gtgtcgcgca gggctgcgga cgacagcagc
     3841 cggatggcgg tcttccagtc ctttgcctcg atggcggcca cgccctgggt gtactcaggg
     3901 tcggagccgc cctcctcggg ttcgccgggt tcggccagcg cgcagccgac cccggcgaac
     3961 aggagcatca gcagaaacct cactgccgcg cggcgagcca ctccttcacc tcgcgcagct
     4021 cggctcggtc gctgtcggct tcctttgtaa ggcgcgccag gttctggtag tagccggccg
     4081 ccttcttcgg cgccccagcc gcctcggcgg ccttggccgc tccgtaatag ccgcgcaagc
     4141 ggttcggcgc cgccttcatc gatgcttcgt attccttgag cgccgcgccc ggctggccgt
     4201 gctcgcgcag catgtcggcg agttgctcgc gcatcgggta gagccggttc tccatcgcca
     4261 cgtgcttctc gctcgaatcc tcgaggtccg cggccgcgcg catgaacttg agcgcctcgt
     4321 ccttgttgcc ctgagcattg gcgagccagg cctgggcgga aaggatctgg atttccacct
     4381 gctccgccca ataggcctgc tttgcttttt caatctgctc gcgcaggcct ttcagctttt
     4441 cgatgtccgc ctgcgccgcg gccgcgtcgc cgctgcgcgc cgcacccatg gcgcgcgcgt
     4501 agtgcgtgat cgcctcggcg gccgggaatg gcgagccgag cggctgcagc gcggcggcgc
     4561 ctttccagtc ctgacgctcc aggaagtagc gcgcgggcac ggcggcgcgg gccatctgcc
     4621 cggcgtggat cggtccgagc actttcttga tctgcgccgt ttcctcgatc agctgccgcg
     4681 ccttgccgtc ctgcccgagc tggagatgcg cgtactgcat gaagtcgttc gcgtgcggca
     4741 cgccgaccag cacgccgtcg agcttcgcct cggcggcgta ctcgcgcgac accacgttcg
     4801 acttgaggtt cgcctcgatc gaatctttcc acatgcccac catcgtgtag atgtgcgagg
     4861 gcatgtgctg cgcgtgcggc gcggagggcg cgatcttcgc gtacttgttg gctgctggaa
     4921 tacccttcct ggcaagcggc gcgaagtcgt agctgtggat caggtaatgc gcgatcccgg
     4981 gatggtcggg atacttcttg tcgagcggct cgaggatctt gatcgcctgc tggagcggcg
     5041 ccatgttctt gtggtcgaag acctcgttca gcgcgagcgc gtggaaaacc ttcgcctcga
     5101 cgtcattcgg gtacttcttg accagcgccg ccatcgcctt ttcgtagttg gcggtgcgcg
     5161 tgtcctgatc tacggcttcg tagttcttgt agaactcctt gatcgccgcg agccagtcgc
     5221 gctcgcgctg cgtcttcgcg ccgatcgcct cgcccttctc gaccgcctcg agcccgcgtt
     5281 tcagggtcgc cgcatcccac ggcccgacca gcgggttggg acggatcgac accgcgaggc
     5341 cccaataggc gatcgcgcag cccgggtcgg tctgcgggat cgccgagaac accttgacgg
     5401 tttccgggaa ccagaacgag tgcagcatgc cgagcgcgcg ctcgaactcc ttctgcgcct
     5461 gcggcgtgca ggaagtcttg aacaggacct tgccgagctt ctcgctcccg accttgtcgt
     5521 gcgcaagcgc tccggtggcg gtgaatgcga acagcaatgc aataagcgtt ttcatagacg
     5581 tcttccttcg gatgggtgat tcagtctacg cccacgcgca acgggtgaga gagtgaccga
     5641 ttcacctccc ccgcactgat aaaattcgag gaatttcagt tatttccgat ggatcgcgcc
     5701 ccttccagct ccaactttat tcggcaaata gtggatgccg acctggccgc cggtacctac
     5761 gccggggtgc gcacgcgctt tccgcccgag ccgaacggct acctgcactt cggccacgtc
     5821 aagtcgatct gcctcaactt cggcctcgcc gagcagtacg gcgggaggtg caacctgcgc
     5881 ttcgacgata ccaacccgga aaaggaggag caggagtacg tcgattccat caaggaaggg
     5941 gtcaagtggc tcggcttcga ctggggacag acggatctct acgcctcgga ctatttcggc
     6001 aagctctacc agttcgccga agcgctcatc gaggccggcc aagcctacgt cgacagccag
     6061 agcgccgagg cgatgagcgc cgcgcgcggc acgttcaccg agcccggcaa ggactcgccc
     6121 tggcgcagcc gcagcgtcga cgagaacctc gcgctgtttc gcggcatgcg cgacggcaag
     6181 tacaaggacg gggcgcacgc gctgcgcgcg aagatcgaca tgcgctcccc gaacctcaac
     6241 ctgcgcgacc cggtgatgta ccgcatccgc cacgcgcacc atcaccgcac cggcgacaag
     6301 tggtgcatct atcccaccta cgactgggcg cacggcctat cggactgcct ggagcaaata
     6361 acccattcgc tgtgcacgct cgagttcgag gaccatcgcc cgctttacga ctggttcaac
     6421 gagcgggtcg ccgaggcggg gctgatccag aaacccttgt cgaagcagat cgagttcgcg
     6481 cgcctgaatc tcacatatgt agtactcagc aagagaagac tcatacagct cgtcgagcag
     6541 aaaatcgtcg acggctggga cgatccgcgc atgcctaccc tcgccggcgc gcgccgccgc
     6601 ggctacacgc cggagggatt ccgcatgttc gccgagcgca tcggcgtgtc gaaggcgcac
     6661 caggtgatcg agttcggcat cctcgaggag tccatgcgcg agcacctgaa cgacgtcgcg
     6721 ccgcggcgca tggcggtgct cgacccggtg aagctcgtga tcgacgacta tcccgaaggc
     6781 aaggaagagc tgatggacct gcccagccac ccgcagaagc ccgagtgggg caagcgccag
     6841 gtcccgttct cgcgagagct ctggatcgag cgcgaggatt ttcaggagaa acccgtaaag
     6901 ggctatttcc ggctgttccc cggaaacgaa gtccgcctgc gctatggcta cgtggtcaag
     6961 tgcatcggca tgaaagacgg cgtcgtgcac tgcacgcacc atccggattc gcgctcaggc
     7021 tcccccggcg cggacaagta caaggtgaag ggcaacatcc actgggtgag cgcgaagcat
     7081 gcgcatgcgg tgaaagtgcg cctgtacgac cggctgttca gagtcgccga gcccggcaag
     7141 gaccgcgatt tccttcagga cctcaatccc gactctcgca agaccgtgga tgcgcaggtc
     7201 gagcccagct tgcgggacag gtctaagcgg caggcttacc agttcgagcg ccacggctac
     7261 ttcatcgccg actacgacgg ggtcacgttc aaccgcacgg tgacgctgcg cgattcctgg
     7321 aaggcgtaga gaaccagagg tacgactgct acgccgggaa aaccacccgg tccggtttcg
     7381 cagtcgtacc cctggagggg agtcaaaggg acttcaggta ctccatcagg tcgacctttt
     7441 ccgggtcggt gagctgcagc ttgaagtgcc ggtcgtagtg ctcgagcact tcggcgagcg
     7501 tggcgaagcg gccgtcgtgg tagaagccgc ccttctggtg cgtccacaac ccggcaagcg
     7561 gcgcggtgcg gtacatccgc gtcggcgagc ggtcggcctg gaaagcgtcg atgcccatct
     7621 ccttcggcga atgcagcgag tgccccggct cggtgaaaag cggcggcaca tggcagctcg
     7681 agcatttcgc cttcgcattg aacagcgctt cgccgcgctt cgcgcccgcg gcgtcgaagg
     7741 tgccggcagc cggcctcggc gccgggatcg agctctggta gaagtgcagc gccgcgagct
     7801 tcggggtgac ctgatcgggg gccttgcgcg tttcggccga gcggctcttc gccgcgaccg
     7861 gatactgctt cttgtcgctc aaacgcttgt cgatgaacgt cccgttgccg tgcatctggg
     7921 tagtgccgac gtaagcattc cagtacggaa ccgagccgaa cccggtccag gtattcaggc
     7981 tcaccccggc gaggccgaac gccgggggaa taagcgttcc cgcctggccg ccatcgggcc
     8041 ggaaggcctt gccgtccttg tcgagcagcg cgtcatagcg gcccggtccc cagctcgcga
     8101 gcaccttctt caccgtcgcc tcgtccactt tcaggagctc ggtgaaaggc ttgaggttgg
     8161 gcgcgagcga gacgatcgcg cctacgttca ggtcgcggtt ggcccagcca tcgaggcgct
     8221 tgccgatgcc gggcgcaaag gaatcgtcga cggtggaatg acacagcgag cactgcacgc
     8281 ccatggagcg cagacgcccg ttgtcgaaga ttcccgtgac gccgaccacg gcattcacct
     8341 tgagcagcgc gagcgtggtc gcggggtctt cgagatcgac cttgcctgcc ttgatttgcg
     8401 ccacgagctc cgacggcagc gcgtcggcgt cgactttgag cccgaccgaa agcgcggcct
     8461 tagggctcag gccgccgccg acgccgccgt tcttcgcgcc ggcgatggcc cggtggagct
     8521 gcagggcgtc gccccagaac gactccgagc ccatggtatc gaagcggaag atgcgtcggc
     8581 cttcgtccag caggcgctgc gcgtgcgcgc tgatctgctt gtcgttctcc gagagctgct
     8641 gttgtccgaa gacgacgccg atgacgctgg cgagcgcagc gaaagcgaga ccggccgggc
     8701 gaagtttcat ggcaatgctc cgcagagaat tcacttgccc attagatgga gcccgcgcgc
     8761 tccccgtttc cggcaccgtc gcaaattcgg ggtcagggtg cgaattcgtt tttacgtacg
     8821 tgaaaactga attcgcaccc tgaccccgaa ttcagttgca gcggtaggtt tccttggggg
     8881 tgcagccctg cccgcgctcg tcgggacacg gaacgaacac gtcccgggtg caaagcgtcg
     8941 caggctcctt cgtgcccttg cgcaggtcga cgcgcgaaaa gcacccaacg catggcggct
     9001 tcataatagc gctatgagca agccagaccc gaagtggaag ctcgatggcg tgcgcgtggt
     9061 gcactcgaac gagctcgaca tcaacacgcc gcagaccccc ggcatgaacc gcgcagcggc
     9121 gattacccgc gcgcgcgccg gtgcagagaa gctttgggcc ggcacggtgg tgatccatcc
     9181 caaggcgaag accggcgcgc accaccacgg agcggtcgaa agcgtcatct atgtcgtgag
     9241 cggaaaagcg cgcatgcgct ggggcgaaaa gctcgagttc gtcgccgagg ccggtcccgg
     9301 ggacttcatc tacgtcccgc cttacgtccc gcaccaggag atcaacgcga gcgacaccga
     9361 gccgctttcg tgcgtgctgt gccgcagcgg ccaggagccg gtggtcgtga atcttccgga
     9421 tctcccggtg gtcgagaaac cggaagaagt gcactgggtg gacaacatcc atcccgaccc
     9481 gacgaaaaaa cctgtataac ggtacccgca ggatttcaag cagcacgcgc gtaccgctct
     9541 catccgtccg ttcgttccct ccttgatcgt ccgcaggcaa cccgccttct actaactgat
     9601 tcaaggaaac agacatgagt accggtaccg taaagtggtt caacgacgcg aagggtttcg
     9661 gcttcatcac ccccgacgac ggcagcaagg atcttttcgt tcatttctcc gccatccagg
     9721 caagcggctt caagacgctg aaggagaacg acaaggtcca atacgaggtc gagcagagcc
     9781 ccaagggccc gcgcgccacg aatatcaagc aggtgtaacg cgccgtcagg cgcaacaaag
     9841 gcccgggctt cccgggcttt tttatttccc taaggccaga gcttgacgcg cggtatgcgg
     9901 ctcgcggtca gcgcgccgag caccgcgacg ccggcggcga cgaggaaggc acgatgaaaa
     9961 gcctccacca catcgacgtg cggcatcagc gcgaacacta ccgcgccgaa tagcgccgct
    10021 cccgtcgcgc ccccggtcga gcgcgcgagc cccatgagcg ccatcgcagc gcccaggcgc
    10081 gcgcggcccg cgacggtctg cacgaccacc tggttgatcg gcattacaac gcccaggccg
    10141 agcccggtca ggaagccgag cgcgatgacg agcccctgat cgtccggcag caaacccagg
    10201 agaagcagcg ccaccgccgc gagcgacatg ccgccgaccg ggatccagtg cgcctctccg
    10261 gtgcggccga ggagcttgga cgcggcgagc gcggcggtga ccatgccggc ggtcaccggg
    10321 agcagcagca ggccggagag cagcgggctc acgcggtggc cgagctgcag gtagatgggc
    10381 aggaagaaga tcaccgcgaa caggctagcg gcgaacagcg ccgtcagcgt cgcggacagc
    10441 ccgaccgtac ggttgcgcag caggtcgacc ggcaggaacg gcgcagcctg ccgtgactca
    10501 tgccaggcga gcgcggcgat gctcacgagc gcgagcgcgg cgagggccag gctccaggtc
    10561 gagccccagg cgaagcggtg cccgacggaa gtcagccagt aaagcgtgct gaccgcggcg
    10621 acggcgaaca gcagctgccc gggcacgtcg cgtccgctcg cggcttcagg atggcgctcg
    10681 cccgcgggca ggcgcgagag gcgccacgcc gcaaacgcgg cgaacggcag gttcgcgaag
    10741 aacagccacc gccagctcgt gtgcgagacc accaggccgc cgagcaccgg gccgccgatg
    10801 ctcgcggtgg tgaacacgag cgcgaaatag ccctggaagc ggatgcgctc gcgcggcggc
    10861 acgagctcgc cgatcagcgc ctgcgacagc gtcatcagcc ctcccccgcc caatccctgc
    10921 agcacgcgcg cggcgacgag ctgcggcagc gtctgcgcca ggccgcacgc cgccgagccg
    10981 agcgcgaaca cgccgagcgc ggcgagcagc acgttgcgcc ggccgtggcg gtcgccgagg
    11041 cgcccgtaca gcgggacgat ggtcgccgag gcaaggagat agccgaccac gatccacgag
    11101 ctgtcgcgca ggtcgccgag cgagccggca atggccggcg tcgccgtggc aagcagcgtc
    11161 tggtctaccg ccgccatgaa catcggcagg aacacggcag cgaacagatt caggaactcg
    11221 gcccgggaga ccgtcgtctt cgggtcggcg cccatttctt acttcggagt ctgggctccc
    11281 accgtaagtc ggcgcatgcg gcggcgctgg ccgtggtagt cgttcaccgc gctgtgttgc
    11341 gtgcagcggt tgtcccatac cgtcagcgtc ccgggcgacc agcgcacgcg gcaggtgaac
    11401 tcgggccgga cctggtgcgc ctgcaggaag tcgatgagcg gccggctctc ctcctcgccc
    11461 atgtcgcgaa agcggatcgt gtggctgcgg gagagataga gcgccttgcg gccggtgtcg
    11521 ggatgcgtgc gcgccaccgg gtgctcggcc tcgtagccgt cggcgtgctc gatgtcctgc
    11581 accttcatgc cgccgatggt gctgtgcatc ttgcggcgcc cgccggtcac cttgaggctc
    11641 gcgctgttga cgccgacgag ctggtccacc attgcgcgca tgccttccga cagcgcgtcg
    11701 taggcggcat aggtgctcgt gaagagcgtg tcgccgccgc gcgccggcgt ctcgagcgca
    11761 tagagcagcg tcgcgagcgg cggctgcggc aggtacgtag tgtcggaatg ccagttgccg
    11821 ccgaagttga cggtctcgct ctcctccttc accacctcga agatgaaagg gaagccttcg
    11881 atcccggtga cgaaggggta gtagcacggc tcgccgaagc gcgcgccgat gcgcatgagg
    11941 tcggcgggct cgagctgctg atcgcgaatc gccaggaccg agtacttcag gaacgcgtca
    12001 tgaatcgctt cccacgtccc gtcgtccttc aggtcgacgc cccggatctc ggccccgagc
    12061 gccccggcca agggctcgat tatcatcggg taactatatg acaaaacgca tcgctatcgt
    12121 cggcgcgggc gcgctcggcg ggtacgtcgg cggcacgctc gcgcacctcg ggcacgacgt
    12181 cacgctgatc gacccgtggc cggagcatgt cgagatcgtc cgcaagcgcg gcctggagct
    12241 ggacggcgtc acccccgaag agaagttcgt cgcccggccg aagatcctcc acctcaccga
    12301 agtccagggg ctggcgaaaa cgccgttcga cgtcgcgatg gtggcgatga agtcctacga
    12361 cacgctctgg gccacggcgc tgatcgcgcc ctacctctcg gagcagggct tcgtggtctc
    12421 gctgcagaac tgcatgaacg aggagacgat cgccggcgtg gtcggctggg ggcgcacggt
    12481 aggcgcggtg gcttcgctca tctcggtcga tctgtacgag gccgggcgaa tccgccgcac
    12541 catcgccaag ggcggcgaca agtacaccat cttccgcatc ggcgagccgc atgggcgaat
    12601 cacgccgcgc atcgagcaac tggtcgaatg gttccgcggc atcgacagcg ccaaagcgac
    12661 gaccaacctc tggggcgagc gctggtcgaa gctggtgcag aactcgatgg gcaacggcgt
    12721 cactgcggcc acgggcatga cctcccccgc ttgcctcggc gacgccaaga tccgccgctt
    12781 ccagatcgcg ctcgccggcg aggcgatccg cgtcgggcag gcgctcggct acacgctcga
    12841 gaaggtgcgc gccgtggagc cggagaagtt cgcgcgtgcg aacgaaggcg atgcgcaggc
    12901 gatggccgaa gtcgaggcgg cgctcatccc caaggccggc gccaacccgc gcggcgacat
    12961 ccagcgtccc tcgatggcgc aggacatcct caagggccgg cgcaccgaga tcgaagcgat
    13021 gaacggctac atcgcgcgca agggcgcaga ggccggcgtg ccggcgccga gccatgcgaa
    13081 gctcgccgcg atcgtcactc ggatcgagcg tggtgaagtc aagccgtcgc cgtctttgtt
    13141 agcttgaggc gaagccccac gggaaacatg gtgaacgcga ggtgctcctg cgcctcgcgc
    13201 ccgtcgggcg tcgtcacctg ctcgatgtcg aagccgccga gcagcatcgc cataaccatc
    13261 ttcatctcga ggatcgcgag gtagcgtcct gggcacaggc gcggcccggc gccgaagggc
    13321 atcgagacgc gcttcggcga gctggctgaa gcgccggtga gccagcgtcc cgggtcgaat
    13381 gcgcccgcgt cgggaaagcg ccgctcgtcg gtcgcgccgg cgcgcatcag gcacatgatc
    13441 agcgtttcct tcggcacctc gatgtccgtg acgaccgtgt cgcgcgtcgg ctgcagcatg
    13501 atgatcggcg ccacgggctt cagccgcatg gtctcgttga tgcaggcatc gatgaacggc
    13561 agcgctgtga cctcctcgta cttctccagg tctttcgagc cgacctcctc gcaggcccgc
    13621 cgcagcgcgt cgggatggcg gtggaggagg tagatcatcc acgccagcgt gttcgcggtg
    13681 gtgtcctcac cggcgagcag catggtcagc acgttgccgg cgacgtcggt gtcgctgagc
    13741 ccgctgcccg gattgtcgcg cgcagcgatc atcgcctcga tgaggttggt cggctgctcg
    13801 cgcagcttcg gattcgcttc catgcggctg cgtgcctggg agatgaaatc gcccaccgcg
    13861 cgatgcagcg cctccaggtg cctgtccagc ttccggtccc tgaaccattc ccagtacggc
    13921 accggcgcca tcaggcgctt gaaaagcgac gggaagatct tgtcgaggtg ggtctggatc
    13981 acgtcctcgt cggattcgag cgtgttgatg tcggcgccga aggcgagccc ggcggtaacg
    14041 tcgaccgtgt agcgcatcag gtcagcctgc agatcgatgt ccaggcgggc cgcc
//
LOCUS       DATBSZ010000041        16071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1097789, whole genome shotgun sequence.
ACCESSION   DATBSZ010000041 DATBSZ010000000
VERSION     DATBSZ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16071
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097789"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1592
                     /locus_tag="VIG70_02765"
     CDS             54..1592
                     /locus_tag="VIG70_02765"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY8249500.1"
                     /translation="MSVLSVRDLRVDFGTLQAVKGISFEIPEHSTVALVGESGSGKSV
                     TALSIVGLLPRNAARSGEISYGGKNLLRASARELRALRGAEISMIFQEPMSSLNPVFS
                     VGFQIGEVLRAHRGLSRREARAASLQLLEEVSLSNPRTKLDAYPHQLSGGEQQRVMIA
                     MAIACEPKLLIADEPTSALDVTVQKQILELIAELQRRRRMSVLFITHDLSLVHIIAAH
                     VIVMREGEIREQGDVAQVFGAPRDDYTRVLLAARPRLDAPSPARSGEETVVFEARGLA
                     KDFGKVKAVKGASFRVARGRTLGLVGESGSGKTTLGLLVARLHRASGGSGLLHGQDAV
                     AVPPKVFKRAVQIVFQNPYASLNPRFSAGEILMEPMRIHGIGAGEAERRGLAAELLAR
                     VGLPSSALFKYPHEFSDDQRQRIAIARSLTLKPQLLICDEAVSALDVSIQAQLLNLLR
                     DLQDEYAMSYLFISHDLAVVKSMADEVMVMKDGEIVEMAGAEQLYSDPKHPYTRTLLA
                     SVPQ"
     gene            1589..3367
                     /locus_tag="VIG70_02770"
     CDS             1589..3367
                     /locus_tag="VIG70_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8249501.1"
                     /translation="MKLVVVAVLLMNCEAWAAHAYSQFGDIKYPAGFSHFEWVNPDAP
                     KGGDIDLVAPLRITNFDKYNPYTLKGTAPPGIGLVFESLLTGTMDEPTTAYGLLAEDV
                     EVAADGLSATFRLDPAARFHDGTPVTAADVKHSFDMLMSKQAAPQYRVVFSEVKQAVV
                     AGPRTVRFDFKSVNAELPLMVGGLPVFSRAWGAGKPFDQVVMDMPITSGPYRIGRLNF
                     GRDITYQRDPNYWARDLNVRRGRYNFDRITYKIYKDNTAQTEAFKAGEFDYLRTFSAR
                     EWARTYVGKKFDSGELIKAELPFKNAGDFQGFLINTRREKFKDPRVREALTLAFDFEW
                     MNRQMMYNSYTRVRGFFNNSDFEAKGLPGPDELKVLLPLKKHLPERVFAKEVPLPPST
                     NPPGSLRDNLRKARDLLAAAGWTYRDGALRNAKGEAFTLEYLDSGGGERLIAPYFQAL
                     AKLGIAGEYRRADFALIRKRLDVFDFDLFTVRVPGSEAPGAELLDRYGSQSADTEGSS
                     NLMGIKDPAIDALLNQAIASHTRPELIARLRALDRVLRHGHFVVPQFYSTSYRIAYRS
                     GKFEQPKVMPQYYQAEDWVISTWWRK"
     gene            3383..4435
                     /locus_tag="VIG70_02775"
     CDS             3383..4435
                     /locus_tag="VIG70_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8249502.1"
                     /translation="MWSYVLRRLLLMIPTLFGILLITFVVIQFVPGGPVEQMVAQFQG
                     FEAGGEGGPGPGAAEGGYRGRQGVDAVRVEEIKKLYGFDKPAPERFAQMIGQFARFDL
                     GKSFFHNKDVWQLVLEKLPVSISLGIWTFLLVYLISVPLGIAKAVRAGTPFDTATTFV
                     ILVGYAIPSFVMGVVLLVFFAGGSFVQWFPLRGITSLNWHELSIPGKILDYFWHIALP
                     VTAMVLGGFAVITILTKNSVLEEIRKQYVLTARAKGVDERRVLWRHVFRNAMIPIMTG
                     FPAAFVGAFFSGSLLIETLFSLDGLGLLSYESVIRRDYPVVFGSLFLFTIIGLLTTLL
                     RDLSYLWVDPRVKFFD"
     gene            4438..5520
                     /locus_tag="VIG70_02780"
     CDS             4438..5520
                     /locus_tag="VIG70_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8249503.1"
                     /translation="MSTPSIAIDFPGEKVVHASPGRRAWRRFRANRLGYFSLVIFAVL
                     FGLSLLAEVLSNDKPLVARYEGSWYFPVLQTLPETTFGGDFATPTDYLDPQIQENMSR
                     GDNFALFPLNRYHHSTINYFASEPSPARPSRENVLGSDDRGRDLLARLLYGFRLSVVF
                     ALMVTVVVSLVGILYGAIQGYFAGWTDIAMERFNEIWSAMPTLYMLIIFSSLFSASFG
                     LLVVLLSVFGWLGIAAYVRAEFLRNRTLDYVRAARALGQSNWKIIRRHILPNSLTPVV
                     TLVPFEMAAAIGALTSLDFLGLGVPPGTPSLGELLNQGKNNLDAWWISLSTFGVLMVM
                     LLLLILIGDALRDALDPRKVLKEDEA"
     gene            5517..7094
                     /locus_tag="VIG70_02785"
     CDS             5517..7094
                     /locus_tag="VIG70_02785"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY8249504.1"
                     /translation="MSLLEVRDLSIDFGSRRVVHNVSFNLDAGEKLALVGESASGKTV
                     TALSTLRLIENARLNGEIFFGGKDVLRMSSPELHQLRGRDIAVIFQEPMTALNPIYTV
                     GAQIVESLVLHTGLRGAAAWAEAVEAMRRVGIDEPERRAHSYPHQLSGGQRQRAMIAM
                     ALACNPKLLIADEPTTALDVTIRLQILELLERLRAEAGMALLLITHDLNLVRRFADRV
                     AVMEKGVLVEQGPTAQVIQRPQHPYTQKLVNSRPGREVGNPGSGTVVTARDVHVHYPT
                     RLPGIRGWFKSGRFTAVESVDFDLAPGETLGIIGESGSGKTTLALAVLGLMAAQGDIR
                     IGKKRWAERAERRSMRKEIQVVFQDPLSSLSPRRTVEQIVGEGLEIHEPGLDAAARRE
                     RILRALYDVGLTEGGQAELLLARYPHEFSGGQRQRIAIARALIVNPKVVVLDEPTSAL
                     DVTIQKQVLELLSSLQRKYELSYILVTHDIDVVRAMAHRVMVMKDSKIVESGPLEQVL
                     ERSQSDYTRRLVEAGYA"
     gene            complement(7172..8476)
                     /locus_tag="VIG70_02790"
     CDS             complement(7172..8476)
                     /locus_tag="VIG70_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY8249505.1"
                     /translation="MFEAERLEIRDRARALPSIDRTAPIDDVWQRIRHGFAMPDLDNA
                     LVREKVRYYASRPDYLQRIFDRSRLYLYHIVEEIEKRGLPTEIALLPMVESAFNPMAY
                     SRAHASGIWQFIPGTGTRFELEQNWWYDGRRDIVQSTNAALDYLTKLYEMHGDWQLAL
                     ASYNWGENAVARAIAKNQAAGKPTDYASLPMPAETRHYVPKLQALKNIVNNPEPFGID
                     IGPIPNQPYFATVTKLRDIDVQLAARLAEMPVEEFIALNPGFSRPYIRASVTPRIVLP
                     ADKVDVFHENLSALGDDSLVSWKTYHPKPGETFESIAKKHRMAVAQLKEVNGIPPRTK
                     AVPRLLVVPTNDSAAAAGTRTLPIMYAPPIPVTTRKIFHTVKSGETLASIARHHRVSV
                     EDLKRWNGVSQATAGRKLAVEVRAAAPKSKPRPKAKPKKRTT"
     gene            complement(8649..10076)
                     /locus_tag="VIG70_02795"
     CDS             complement(8649..10076)
                     /locus_tag="VIG70_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY8249506.1"
                     /translation="MKKLVLPALVLLLAACATVQDAPPVPEPAPEPVAVPAPPPLPIA
                     VAPETKKPEPKPVVTKPKPFDAKPVVVEPPPEIVGIDRAKEPTDLWLRIRKGFAIPDL
                     QSPLVRQKTKQYAARPEYLQRILERSKLYLYHIVEEIEKRGMPTELALLPMVESAFNP
                     MAYSRAHASGIWQFIPGTGKRFELEQNSWYDGRRDIVDSTNAALDYLTKLHQMYGDWQ
                     LALASYNWGENAVRKAIARNKAAKKKTDYASLRMPKETRHYIPKLQALENIIANPSAF
                     GVDLDAIPNQPYFAVINEIPDIDVHVAARLAEMPMEEFIALNPGFSRPLIRSSAARRI
                     ILPADKLVAFYDNLDQLDEADLVSWEVYHPERGEKLEAIAKRFEMSLAELKRVNGIHS
                     SSSRVPAELVVPKNGDARATLADLPLMYAPPIPPKGTVRAVHTVKQGDTLGSIAKRYR
                     VSIDSLKRGNNVGRFLQVGQKIYIR"
     gene            complement(10073..10837)
                     /gene="gloB"
                     /locus_tag="VIG70_02800"
     CDS             complement(10073..10837)
                     /gene="gloB"
                     /locus_tag="VIG70_02800"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEY8249507.1"
                     /translation="MLEVVPVKAFKDNYVWTLRNATHAAVVDPGEAGPVLDYLAREKL
                     GLAAILATHHHPDHVGGVAQLLEKYRVPVYGPKNEPIPTLTQPVSEGDTVTIPALGVS
                     FSVLDIPGHTRAHIAYYGAGAIFCGDTLFACGCGRVFEGTAEQMYASLGKLRALPDET
                     KVYCGHEYTLANIRFAKGVEPQNAALAAREARDRRLQEAGKPTLPSTMREEKATNPFL
                     RCTEPAVLESVNKYLGTRVSDPVRVFAAIREWKNRS"
     gene            10836..11603
                     /locus_tag="VIG70_02805"
     CDS             10836..11603
                     /locus_tag="VIG70_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8249508.1"
                     /translation="MRQPFVQNGALKYNGGRSSLSGWLSTPQGAYVLDWELAQFDSAV
                     DDVFGFRAVQVGLPEVDFLRQNRINYRFSLALEPGAALAADPLQLPLASQSVDLVALP
                     HVLEGHHNPHDVLREVERVLMPEGQIVISGFNTASLWRVRQAFTFHRNGAPWDAKFIG
                     LFRLREWLRVLGFELNGGKLGCYAPPFSDQAWLQRFAFMEKAGARWWPVTGGVYVVRA
                     VKRVHGMRIVTPAWRRERARRRQLAPVSQRSHKHHHE"
     gene            11596..12057
                     /gene="rnhA"
                     /locus_tag="VIG70_02810"
     CDS             11596..12057
                     /gene="rnhA"
                     /locus_tag="VIG70_02810"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY8249509.1"
                     /translation="MSDPAGEAVVEIWSDGACRGNPGPGGWGVLLRSNGAEKELYGGE
                     ADTTNNRMELMAAIRALEALKRPSKVKLHTDSTYVMKGITTWIHDWKKKGWRTADKKA
                     VKNEDLWRQLEAVAAKHEVEWHWVKGHAGHPENEWADRLANKGIPGAADRP"
     gene            12038..12742
                     /gene="dnaQ"
                     /locus_tag="VIG70_02815"
     CDS             12038..12742
                     /gene="dnaQ"
                     /locus_tag="VIG70_02815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEY8249510.1"
                     /translation="MRQIVLDTETTGLNPKLGDRIIEIGCVELLSRRLSEREFHAYLN
                     PEREVELGATRVHGLTREDLAAKPKFGEIAREFLDYIRGAEVIIHNADFDVEFLNMEL
                     ERAGLGRVQDHVAAITDTLLSAKELHPGKKNSLDALCERYFVDNSRRTLHGALADARL
                     LAECYLAMTRGQESLMMDLEAPAAAAAAAAGLILDVSKLIVQRALPDELATHEQYLDA
                     MEKDAKRAAVWRRLVS"
     gene            complement(12739..13494)
                     /locus_tag="VIG70_02820"
     CDS             complement(12739..13494)
                     /locus_tag="VIG70_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249511.1"
                     /translation="MRWAALLNKSYRLLRLAVLGVVAAGLLAVLLFARPELAAWLALA
                     LALPAFAMAYLALVKAKMLEFCPEVLGGDVILPRAAISGGRVRLLLPLQFNNTGHADG
                     IIEWIAIRLTLDGRSDQSLLLSPVAEVDMQRFIQAKRMLEEGNTIEPFTSFALEGKRT
                     AAKFVLFDHAEKNRARPLELRPGRYSFELFIKATHSHQPQLARSFEHVLETQQLEDFR
                     GGNTVYLFNYDLTLPGVRRAVAGAEWLPRASAA"
     gene            13584..15224
                     /locus_tag="VIG70_02825"
     CDS             13584..15224
                     /locus_tag="VIG70_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEY8249512.1"
                     /translation="MFPKANAYERDLDKNAANYAPLTPISFLERAAYVYPDKVSVVHG
                     KERYTWKETYARCRRLASALAKRGIGAGDTVAVMLPNIPAMYEAHFGVPMCGAVLNTL
                     NTRLDAEAIGFMLEHGEAKLLLADAEFAPVLEKAIKGRNITVIDVEDPEFSGGKRLGK
                     TTYEELLEEGDPDFAWRWPEDEWEAISLNYTSGTTGNPKGVVYHHRGAYLNGVGNILV
                     WGMPHHAIYLWTLPMFHCNGWCFPWTMAANAGTSICLRRVEAGPIFDLIKKHRVTHYC
                     GAPVVHQLLINAPEEMKRGIEHQVNGLVAAAAPPAAMIEGMQKIGFNITHVYGLTEVY
                     GPATVCAKHPEWERLPLADQVRLNGRQGVRYHVEEGLTVMDPETMQPVPPDGETMGEI
                     MFRGNITMKGYLKNPKATRDAFAGGWFHSGDLAVMQPDGYIKIKDRSKDIIISGGENI
                     SSLEVEDVLYRHPAVMAAAVVARPDPKWGETPCAFVELKPGVAVSETEIIEFCRGQLA
                     KFKAPRAVVFGELPKTSTGKIQKFVLREKAKSTAAIDT"
     gene            15221..16012
                     /locus_tag="VIG70_02830"
     CDS             15221..16012
                     /locus_tag="VIG70_02830"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY8249513.1"
                     /translation="MSAVPQAEQPILLREDRDGICTLTMNRPQQLNLLTRDMLSALQD
                     AFDSLKSDKQTRVVVLAGTGKGFCAGHDLKEIKALKELPKIEALFGQCSRMMQTIPLL
                     PQPVIARVQGAAAAAGCQLVAQCDLAIASDAAKFVTSGVTWGFFCSTPGVAVGRNLQR
                     KHAMEMLLTGEPISATRAMEWGLVNKVVPLDQLDHAVMDLAKTLASKPPATVAAGKRA
                     FYQQMDLGLEQAYALASRVISASFAHEEGRAGMDAFIDKRPPPKH"
BASE COUNT         2701 a         5170 c         5425 g         2775 t
ORIGIN      
        1 gccttcagcc tgtttaccga ctccgtgcgc gacgcgctcg atccgaggct gcaatgagcg
       61 tgctctcggt gcgggacctg cgagtcgatt tcggcacgct tcaggctgtg aagggcatca
      121 gcttcgagat cccggagcac agcacggtgg cgctggtcgg ggagtccggc agcgggaagt
      181 cggtcactgc gctttccatc gtcggcctgc tcccccgcaa cgcggcccgg agcggcgaga
      241 tctcctacgg cggaaagaac ctcctgcggg cctcggcgcg cgagctgcgc gcgctgcgcg
      301 gcgccgagat ctcgatgatc ttccaggagc cgatgagctc gctgaacccg gtgttcagcg
      361 tcggtttcca gatcggcgag gtgctgcgcg cgcaccgagg cctttcgcgg cgcgaggcac
      421 gagcggcttc gttgcagttg ctcgaggagg tcagtctttc aaatccaagg accaagctcg
      481 atgcctatcc gcaccagctc tccggcggcg agcagcagcg ggtgatgatc gccatggcga
      541 tcgcctgcga gcccaagctc ctgattgccg acgagcccac cagcgcgctc gacgtcaccg
      601 tgcagaagca gatcctcgag ctgatcgcag agctgcagcg ccggcgccgc atgtcggtgc
      661 ttttcattac ccatgatttg tctctggtcc acatcattgc cgcgcacgta atcgtcatgc
      721 gcgaaggcga gatccgcgag cagggggacg tggcgcaggt cttcggcgcg ccgcgcgacg
      781 actacacgcg cgtgctgctc gccgcccggc cccgcctgga cgcgccgtcc ccggcgaggt
      841 ccggggagga aaccgtggtc ttcgaggccc gcggcctggc gaaagacttc ggcaaggtca
      901 aggccgtcaa gggcgcgagc ttccgcgttg cccgggggag gacgctcggc ctggtcggcg
      961 agtcgggctc gggcaagacc accctcgggt tgctcgtcgc acggctgcat cgggcctcgg
     1021 gcggctcggg gttgctccac gggcaggacg ctgttgcggt tcctcccaag gtcttcaagc
     1081 gcgcggtcca gatcgtgttc cagaatccct atgcttcgct caatccccgc ttcagcgccg
     1141 gcgagatcct catggagccc atgcgcatcc acggcatcgg cgccggcgag gccgagcgcc
     1201 gcggcctggc cgcggaattg cttgcgaggg tcgggctgcc gtccagtgcg ctcttcaagt
     1261 accctcacga gttctccgac gaccagcgcc aacggatcgc gatcgcgcgg tcgctgacgc
     1321 tcaagccgca gctcctgatc tgcgacgagg cggtttcggc gctggatgta tcgatccagg
     1381 cgcagctttt gaacctgctg cgggacctgc aggacgaata cgcaatgagc tatctcttca
     1441 tctcgcacga tctcgccgtg gtgaagtcca tggccgacga ggtcatggtg atgaaggacg
     1501 gcgagatcgt cgagatggcc ggcgccgagc agctctattc cgacccgaag cacccctata
     1561 cgcgtacgct tctcgcttcg gtcccgcaat gaaactcgtc gttgtggcgg tgctgttgat
     1621 gaactgcgag gcgtgggccg cccacgccta ttcgcaattc ggcgacatca agtatccggc
     1681 cgggttcagc catttcgagt gggtgaatcc ggacgcgccc aaaggcggcg acatcgatct
     1741 ggtggcaccg ctgcgcatca cgaacttcga caagtacaac ccctacaccc tgaaagggac
     1801 ggcgccgcct ggaatagggc tggtgttcga aagcctgctg accggcacga tggacgagcc
     1861 gaccaccgcc tacggccttc tcgccgagga tgtcgaggtc gcggccgacg gcctgtccgc
     1921 gaccttccgc ctcgacccgg cggcgcgctt ccacgacggc acgccggtca cggcggcgga
     1981 cgtcaagcac agcttcgaca tgctgatgag caagcaggcg gcgccccagt accgcgtggt
     2041 gttcagcgag gtgaagcagg cggtggtcgc agggccgcgc accgtgcggt tcgatttcaa
     2101 gagcgtcaat gccgagctgc cgctgatggt cggcgggctg ccggtgttca gccgcgcctg
     2161 gggggccggc aagcccttcg accaggtggt gatggacatg ccgatcacca gcgggccgta
     2221 caggatcggg cggctcaact tcggccgcga catcacctac cagcgcgacc cgaactactg
     2281 ggcgcgcgac ctgaacgtgc ggcgcggacg ctacaacttc gaccgcatca cctacaagat
     2341 ctacaaggac aacaccgcgc agaccgaggc cttcaaggcg ggggagttcg actacctgcg
     2401 cacgttctcg gcgcgcgaat gggcccggac ctatgtcggc aagaagttcg actcgggcga
     2461 gctgatcaag gccgagctgc cgttcaagaa cgccggcgac ttccagggct tcctcatcaa
     2521 cacgcggcgc gagaagttca aggacccgcg ggtgcgcgag gcgctcacgc tcgccttcga
     2581 cttcgagtgg atgaaccggc aaatgatgta caactcgtac acgagggtgc gcgggttctt
     2641 caacaacagc gacttcgaag cgaaggggct gccggggccc gacgagctga aggttctcct
     2701 tccgctgaaa aagcacttgc cggaacgggt gttcgcgaag gaggtgccgc tgccgccctc
     2761 caccaacccg ccgggcagcc tgcgcgacaa cctgcgcaag gcgcgcgacc tgcttgcggc
     2821 cgcgggatgg acctaccgcg acggcgcgct gcgcaacgcc aagggcgaag cgttcacgct
     2881 cgagtacctc gacagcgggg gcggtgagcg cctgatcgcg ccgtatttcc aggcgctggc
     2941 gaagctcggt atcgcggggg agtaccggcg ggcggacttc gcgctgatcc gcaagcgcct
     3001 cgacgtgttc gacttcgacc tcttcacggt gcgcgttccc ggcagcgagg cgccgggcgc
     3061 cgagctgctc gaccgctacg gctcgcaatc ggccgatacc gagggctcga gcaacctgat
     3121 gggcatcaag gatccggcga tcgacgcgct gctgaaccag gcgattgcct cgcacacgcg
     3181 gcccgagctc atcgcgcgcc tcagggcgct cgaccgcgtg ctgcgccacg gccacttcgt
     3241 ggtgccgcag ttctactcga ccagctaccg catcgcgtac cgctccggga agttcgagca
     3301 gccgaaggtc atgccgcagt actaccaggc ggaagactgg gttatttcga cctggtggag
     3361 aaaatagaag gaaaacgccc cgatgtggtc ctacgtcctc aggcgccttc tgctgatgat
     3421 cccgaccctg ttcgggatcc tgctcatcac tttcgtggtg atccagttcg tgcccggcgg
     3481 cccggtcgag cagatggtgg cgcagttcca gggcttcgag gcgggaggcg aaggcggccc
     3541 gggccctggg gcggccgagg gtggttaccg aggcaggcag ggcgtggacg cagtgcgcgt
     3601 cgaggagatc aagaagctct acggcttcga caagccggcg cccgagcgct tcgcgcagat
     3661 gatcggccag ttcgcgcgct tcgacctggg caagagcttc ttccacaaca aggacgtctg
     3721 gcagctcgtg ctcgagaagc tgccagtgtc gatcagcctg gggatatgga ctttcctcct
     3781 ggtgtacctg atctcggtgc cgctcggcat cgccaaggcg gtgcgcgccg gcacgccgtt
     3841 cgacaccgcc accaccttcg tcattctcgt gggctacgcc atcccgagct tcgtgatggg
     3901 cgtggttctg ctcgtgttct tcgccggcgg ctcgttcgtg cagtggttcc cgctgcgcgg
     3961 cattacctcc ctgaactggc acgagctgag cattccagga aagatcctcg actacttctg
     4021 gcatatcgcg ctgccggtca cggcgatggt cctcggcggg ttcgcggtga tcaccatcct
     4081 caccaagaac tcggttctgg aggagatccg caagcagtac gtcctgaccg cgcgcgccaa
     4141 gggcgtggac gagcgccgcg tgctgtggcg gcacgtattc cgcaacgcga tgatcccgat
     4201 catgaccggc ttcccggcgg cgttcgtcgg tgcgttcttc agcggctcgc tgctgatcga
     4261 gacgctgttc tcccttgacg ggctggggct cctttcctac gagtcggtga tccgccgcga
     4321 ttaccccgtg gtgttcggca gcctcttcct cttcaccatc atcggccttc tcacgacgtt
     4381 gctgcgggac ctgagctacc tctgggtcga tccgcgtgtg aagttcttcg actgagcatg
     4441 tcgacgcctt ccatcgcgat cgattttccc ggggagaagg tggtccacgc gagtcccgga
     4501 cgccgggctt ggcggcgctt ccgcgccaac cgtctggggt attttagttt ggtcattttc
     4561 gcggttctct tcggcctgag cctgctcgcc gaggtcctct cgaacgacaa gccgctggtc
     4621 gcgcgctacg agggaagctg gtacttcccg gtgctgcaga cgctgccgga gacgaccttc
     4681 ggcggcgatt tcgccacgcc caccgactat ctcgatccgc agatccagga gaacatgtcg
     4741 cgcggcgaca acttcgcgct tttcccgctc aaccgctacc accacagcac gatcaactat
     4801 ttcgcttccg agccgagccc ggcgcggccg agccgcgaga acgtgctggg cagcgacgac
     4861 cggggccgcg acctgctggc acggctgctg tacggcttcc ggctgtcggt ggtgttcgcg
     4921 ctcatggtga cagtggtggt ttccctggtc gggatcctgt acggcgcgat ccaggggtat
     4981 ttcgcgggat ggaccgacat cgccatggag cgcttcaacg agatctggag cgcgatgccc
     5041 acgctctaca tgctgatcat cttctcgtcg ctgttctcgg cgagcttcgg cctgctggtg
     5101 gtgctgctca gcgtgttcgg ctggctcggc atcgcggcgt acgtgcgtgc cgagttcctg
     5161 cgcaaccgca cgctcgacta cgtgcgcgcg gcgcgcgcgc tgggccagag caactggaag
     5221 atcatccgcc gccacatcct gccgaacagc ctaacgccgg tggtgacgct cgtgcctttc
     5281 gagatggcgg cggcgatcgg cgcgctcacc agcctggact tcctcggcct gggcgtgccg
     5341 ccggggacgc ccagcctcgg cgagctgctc aaccagggga agaacaacct cgacgcctgg
     5401 tggatctcgc tctccacctt cggcgtgctg atggtgatgc tgctgctcct catcctgatc
     5461 ggcgacgcgc tgcgcgatgc gctcgatccg aggaaggtgc tcaaggagga cgaggcatga
     5521 gcctcctcga agtccgcgac ctcagcatcg acttcggctc gcgccgggtg gtgcacaacg
     5581 tgtccttcaa cctcgatgcg ggcgaaaagc tcgcgctggt gggggaatcg gcttccggga
     5641 agacggtgac cgcgctgtcc acgcttcggc tgatcgagaa cgcgcgcttg aacggggaga
     5701 tctttttcgg cggcaaggac gtgcttcgca tgtcctcacc tgaattgcat caattgcgcg
     5761 gccgggacat cgcggtgatc ttccaggagc cgatgaccgc gctgaacccg atctacacgg
     5821 tcggcgccca gatcgtcgaa tcgctggtgc tgcacaccgg gctgcgcggc gccgcggcgt
     5881 gggccgaggc ggtggaagcg atgcggcggg tgggcatcga cgagcccgag cgccgcgcgc
     5941 acagctaccc gcaccaactc tcgggcggcc agcgccagcg cgccatgatc gccatggcgc
     6001 tcgcgtgcaa tccgaagctc ctgattgcgg atgagcccac caccgcgctc gacgtcacga
     6061 tccggctgca gatcctcgag ctgctggagc ggctgcgcgc cgaggcgggc atggcgctgc
     6121 tcctcatcac gcacgacctg aacctggtgc ggcggttcgc cgaccgggtc gccgtaatgg
     6181 agaagggggt gctggtggag cagggcccca ccgctcaagt gatccagcgg ccccagcacc
     6241 cctataccca gaagctcgtg aacagccggc cgggccgcga agtcggcaat ccgggcagcg
     6301 ggacggttgt aaccgcaaga gacgtgcatg tgcactaccc gacgcggctg cccggcatcc
     6361 gcggctggtt caagagcggg cggttcaccg cggtcgagtc ggtcgatttc gacctcgcgc
     6421 ccggtgagac gctcggcatc atcggcgaat cgggctcggg caagacgacc cttgcgcttg
     6481 ccgttctcgg cctgatggcc gcgcagggcg acattcgcat agggaagaag cgctgggcgg
     6541 agcgtgcgga gcgacgcagc atgcgcaagg agattcaggt cgtttttcag gacccgctgt
     6601 cgtcgctgtc gccgcgacgg acggtggagc agatcgtcgg cgagggcctc gagatccacg
     6661 agcccgggct cgacgcggcg gcaaggcgcg agcgcatcct gcgggcgctg tacgacgtcg
     6721 gcctcaccga gggcgggcag gccgagctgc tgctcgcgcg ctatccccat gaattctcgg
     6781 gcgggcagcg ccagcgcatc gccatcgccc gagcgctcat cgttaacccg aaggtggtgg
     6841 tgctcgacga gcccaccagc gcactcgatg tcacgatcca gaagcaggta ctcgagcttc
     6901 tatcgtctct gcaaagaaaa tacgaactga gctacatcct ggtcacccac gacatcgacg
     6961 tagtgcgcgc catggcgcac cgcgtgatgg tgatgaagga cagcaagatc gtggagagcg
     7021 ggccgctcga gcaagtgctc gaaaggtcgc agtccgacta cacgcgccgg ctggtcgaag
     7081 ccgggtacgc ctaagtcgtt tgtgaattcg gggacggagc cctaactcca cggctggcgc
     7141 agctttcgga gttagggctc cgtccccgaa ttcaagtcgt ccgcttcttg ggtttcgcct
     7201 ttgggcgcgg cttgctcttg ggcgcggcgg cgcgcacctc gaccgcgagc ttccgcccgg
     7261 ccgtcgcctg gctgacgccg ttccagcgct tgaggtcctc gaccgagact cggtggtgcc
     7321 ttgcgatcga ggccagggtt tcaccggact tcacggtatg gaagatcttg cgcgtcgtca
     7381 ccgggattgg cggcgcgtac atgatcggca gcgtgcgcgt cccggccgcg gcggcgctgt
     7441 cgttggtcgg cacgacgaga aggcgcggca ccgccttggt gcgcggcggg atgccgttca
     7501 cctccttcaa ctgcgcgacc gccatgcggt gcttcttggc gatcgattcg aaggtttcgc
     7561 ccggcttggg gtgataggtt ttccaggaca ccagcgagtc atcgcccaag gcgctcaggt
     7621 tctcgtggaa cacgtcgacc ttgtccgccg gcagcacgat gcgcggcgtg accgaggcgc
     7681 ggatgtaggg ccggctgaag cccggattga gggcgatgaa ctcctcgacc ggcatctcgg
     7741 cgaggcgcgc ggcgagctgc acgtcgatgt cgcgcagctt ggtgacggtc gcgaaatagg
     7801 gctgattggg aatcggcccg atgtcgatgc cgaagggctc gggattgttg acgatgttct
     7861 tcagcgcctg gagcttgggg acgtagtggc gcgtctccgc cggcatcggc aggctcgcgt
     7921 agtcggtcgg cttgcccgcg gcctggttct tggcgatagc gcgcgccacg gcgttctcgc
     7981 cccagttgta cgaggcgagc gcgagctgcc agtcgccgtg catctcgtag agcttggtga
     8041 ggtagtcgag cgcggcattg gtcgactgga cgatgtcgcg gcggccgtcg taccaccagt
     8101 tttgctcgag ctcgaagcgc gtgccggtgc cggggatgaa ctgccagatc cccgaggcat
     8161 gcgcgcgcga gtacgccatc ggattgaacg cgctttccac catcggcagc agcgcgatct
     8221 cggtgggcag gccgcgcttc tcgatctcct cgacgatgtg atagagatac aggcggctgc
     8281 ggtcgaagat gcgctgcaga tagtcgggcc gtgacgcgta gtaccggacc ttctcccgca
     8341 ccagcgcgtt gtcgaggtcg ggcatggcga agccgtggcg aatgcgctgc cacacgtcgt
     8401 cgatcggcgc ggtgcggtcg atgctcggca gcgcgcgcgc ccgatccctg atttcgagcc
     8461 gttcggcttc gaacaccagc ggcgggaggg gctttgctgc cggggctgcg cgggcaggtg
     8521 gtgcttcgct ggtcgctgca ggaatcggat cgggagcaga ttccaaagac ggctcgggcg
     8581 cctgcggttc catggcgcac gcactcagca gcagcgctgc cagggtcggg cgaagaagct
     8641 tcacccgctt accttatata gattttctgt ccgacctgca ggaagcggcc gacgttgttg
     8701 ccgcgcttga gggaatcaat gctgacccgg tagcgcttgg cgatcgagcc cagcgtgtcg
     8761 ccctgcttca cggtatgcac cgcgcgcacc gtgcccttgg gcgggatcgg cggcgcgtac
     8821 atcagcggca gatcggcgag ggtcgcgcgc gcgtcgccat tcttcggtac gaccaattcg
     8881 gccggcacgc gcgaggaact cgagtgaatg ccgttcacgc gcttgagctc ggcaaggctc
     8941 atctcgaagc gcttggcgat ggcttccagc ttctcgccgc gctccgggtg atagacctcc
     9001 caggagacga ggtcggcttc gtcgagctgg tcgaggttgt cgtagaacgc caccagcttg
     9061 tcggcgggca ggatgatgcg ccgggccgcc gaggagcgga tgagcggccg gctgaagccg
     9121 ggattgagcg cgatgaactc ctccatcggc atctcggcca gcctcgccgc gacgtgaacg
     9181 tcgatgtccg ggatctcgtt gatcaccgcg aaatacggct ggttcggaat cgcgtcgagg
     9241 tcgacgccga acgccgaggg attggcgatg atgttctcga gcgcctgcag cttcgggatg
     9301 tagtgccgcg tctccttcgg catgcgcagg ctcgcgtagt cggtcttctt cttcgcggcc
     9361 ttgttgcgcg cgatcgcctt gcgcacggcg ttctcgcccc agttgtagga cgcgagcgca
     9421 agctgccagt cgccatacat ctggtgcagc ttggtgaggt agtcgagcgc tgcgttggtg
     9481 gaatcgacga tgtcgcggcg cccgtcgtac cacgagttct gctcgagctc gaagcgcttg
     9541 ccggtgccgg ggatgaactg ccagatgccc gaggcgtgcg cgcgcgaata ggccatcgga
     9601 ttgaacgcgc tttccaccat cggcagcagc gcgagctcgg tcggcatccc gcgcttctcg
     9661 atctcttcga cgatgtggta gaggtagagc ttgctgcgct ccaggatccg ctgcaggtac
     9721 tccgggcgcg cggcgtattg cttggtcttt tgccgcacca gcgggctctg caggtcggga
     9781 atcgcgaatc ccttgcggat gcgcagccac aggtcggtcg gttccttcgc gcggtcgatg
     9841 ccgacgatct cgggcggcgg ctcgaccacg acgggcttgg cgtcgaaagg cttgggcttc
     9901 gtgacgaccg gcttcggttc cggcttcttc gtctcgggcg cgaccgcgat cggaagtggc
     9961 ggcggcgcag gcacggcgac cggctccggc gccggctcag ggacgggcgg ggcgtcttgc
    10021 accgtcgcgc aggccgcgag cagcaggacc aaggccggaa ggacgagctt tttcaagagc
    10081 ggtttttcca ctcgcgaatc gcggcaaata cccgcacagg atcagaaacg cgcgtaccga
    10141 ggtatttgtt gaccgattcc agcaccgcgg gctcggtgca acgcaggaag gggttggtgg
    10201 ccttttcctc gcgcatcgtg ctcggcaggg tgggcttgcc cgcttcctgc agccggcggt
    10261 cgcgggcctc gcgggccgcg agcgcggcgt tctgcggctc gacgcccttc gcgaaacgga
    10321 tgttggcgag cgtgtattcg tgcccgcaat acaccttggt ctcgtcgggc agcgcgcgca
    10381 gttttccaag cgaggcatac atctgctcgg cggtgccttc gaacacgcgc ccgcagccgc
    10441 aggcaaagag cgtgtcgccg cagaagatcg cgcctgcccc ataataggca atatgcgcgc
    10501 gcgtatggcc gggaatgtcg agcaccgaga aggagacgcc gagcgcggga atcgtcaccg
    10561 tgtcaccctc ggagacgggc tgggtgagcg tgggaatcgg ctcgttcttc ggcccataga
    10621 cggggacgcg gtatttttcc aggagttgcg ccacgccgcc gacgtggtcg ggatggtgat
    10681 gcgtggcgag gatcgcggcg aggccgagct tctcgcgcgc gagatagtcg agcaccggcc
    10741 cggcttcgcc ggggtcgacc actgcagcgt gcgtcgcgtt gcgcagcgtc cagacatagt
    10801 tgtccttgaa cgccttgact ggaacgactt cgagcatgcg tcaacccttc gtccaaaacg
    10861 gggcgctgaa gtataacggc ggtcgttcat cgctttctgg gtggctctct accccccagg
    10921 gagcgtacgt gctcgattgg gagctcgccc agtttgacag cgctgttgat gacgtgttcg
    10981 gtttcagggc agtacaggtc ggcctaccgg aagtcgattt tttgcgacag aaccgcatta
    11041 attaccggtt cagccttgcg ctggagccgg gggcggcgct ggccgccgat ccgctgcagc
    11101 tcccgctcgc ctcgcaaagt gtggatttag tcgcactgcc gcacgtgctg gaagggcatc
    11161 acaatccgca cgacgtgctg cgcgaggtcg agcgcgtgct gatgccggag ggccagatcg
    11221 tgatctccgg cttcaacacg gcctcgctct ggcgcgtgcg ccaggcgttc acgttccacc
    11281 gcaacggcgc cccctgggac gcgaaattca tcggtctctt tcgcctgcgc gaatggctgc
    11341 gggtgctcgg cttcgagctg aacggcggca agctcggctg ctatgcgccg cccttcagcg
    11401 accaggcctg gctgcagcgc ttcgccttca tggaaaaagc cggcgcgcgc tggtggccgg
    11461 tgaccggggg ggtttacgtc gtccgcgcgg tgaagcgggt gcacggcatg cgcatcgtca
    11521 cgccggcgtg gcgccgggaa cgcgcgcgcc ggcgacagct cgcgccggtg tcgcagagga
    11581 gccacaagca tcaccatgag tgatcccgcc ggtgaggccg tggtggagat ctggagcgac
    11641 ggggcgtgcc gcggcaatcc gggtccgggc ggctggggtg tcttgctgcg ctccaacggg
    11701 gcggaaaagg agctgtacgg cggcgaggcc gataccacga acaaccgcat ggagctgatg
    11761 gcggcgattc gcgccctcga ggccttgaaa cgcccgagca aggtcaagct ccacacggat
    11821 tcgacttacg tgatgaaggg catcaccacc tggatccacg actggaagaa aaaaggctgg
    11881 cgcaccgcgg acaagaaggc ggtgaagaac gaggacctgt ggcggcagct cgaggcggtg
    11941 gccgcgaagc acgaggtcga atggcactgg gtgaaggggc atgccggcca ccccgagaac
    12001 gaatgggcgg acaggctggc gaacaaggga attccgggtg cggcagatcg tccttgacac
    12061 ggaaaccacc gggctgaatc cgaagctcgg cgaccgcatc atcgagatcg gctgcgtcga
    12121 gctcctcagc cggcgcctga gcgagcgcga gttccacgcc tacctcaacc cggagcgcga
    12181 ggtcgagctc ggggcgacgc gagtgcacgg cctgacgcgc gaggacctcg ccgccaagcc
    12241 gaagttcggc gagatcgcgc gcgagttcct cgactacatc cgcggcgcgg aggtgatcat
    12301 ccacaacgcg gacttcgacg tcgagttcct caacatggag ctcgagcggg cgggcctggg
    12361 ccgggtgcag gaccacgtcg cggcgatcac cgacacgctg ctctcggcca aggagctgca
    12421 tcccggcaag aagaactcgc tcgacgcgct gtgcgagcgc tacttcgtcg acaactcgag
    12481 gcgcaccctg cacggcgcgc tcgccgacgc gcggctgctc gccgagtgct acctcgccat
    12541 gactcgcggc caggagagcc tgatgatgga cctcgaggcc ccggccgcgg cggccgcggc
    12601 ggcggcgggg ctgatcctcg acgtgtcgaa gctgatcgtg cagcgcgcct tgcccgacga
    12661 gcttgccacg cacgagcaat acctcgacgc catggaaaaa gatgccaagc gcgccgcggt
    12721 gtggcgccgg ctggtcagct aggcggcgga ggcgcgcggc agccattcgg ctcccgcgac
    12781 tgcccggcgc acgcctggaa gcgtgaggtc gtagttgaag aggtaaaccg tgttcccgcc
    12841 gcgaaaatct tcgagctgct gcgtctccag cacgtgctcg aatgagcgtg cgagctgcgg
    12901 ctgatgcgag tgcgtcgcct tgatgaagag ctcgaagctg tagcgtcccg ggcgcagctc
    12961 gagcggcctg gcgcggttct tctccgcgtg gtcgaagagc acgaacttgg cggccgtgcg
    13021 cttgccctcg agcgcgaagc tggtaaaggg ctcgatcgta ttgccctctt cgagcatgcg
    13081 cttcgcctgg atgaaccgct gcatgtccac ctcggccacc gggctcagga gcagcgactg
    13141 gtcgctgcgc ccgtcgagcg tcaggcggat cgcgatccac tcgatgatgc cgtcggcatg
    13201 gcccgtgttg ttgaactgca gcggcaggag gagccgcacc cggccgccgc tgatcgcggc
    13261 gcgcggcagg atgacgtcgc cgcccagcac ctcggggcag aactccagca tcttcgcctt
    13321 gacgagagcc aggtatgcca tcgcgaatgc cggcaaagcc agcgcaagcg ccagccacgc
    13381 ggcgagttcg gggcgagcga acagcaacac ggccagcaaa ccggcggcga ccacgcccag
    13441 caccgccagc cgcagcaggc ggtaagactt gttgagcaga gctgcccagc gcatcaggag
    13501 attcgacgcg ttgacgcatg aatcttctac ccatggcctg cgacacccgg cctatggcgg
    13561 cggccgaggc tatactttgc gccatgtttc caaaggcaaa tgcctacgag cgcgacctcg
    13621 acaagaacgc ggcgaattac gccccgctga cgccgatctc cttcttggag cgcgccgcct
    13681 acgtgtaccc ggacaaggtt tccgtggtcc acggcaagga gcgctacacg tggaaggaaa
    13741 cgtatgctcg ctgccgccgg ctggcctctg cgctggcgaa gcgcgggatc ggcgccggcg
    13801 acaccgtcgc cgtcatgctg ccgaacatcc cggcgatgta tgaggcccat ttcggcgtgc
    13861 cgatgtgcgg cgcggttctc aacactctga acacccgcct tgacgccgaa gcgatcggct
    13921 tcatgctcga gcacggcgaa gcgaagctcc tgctggcgga cgcggaattc gccccggtcc
    13981 tcgaaaaagc catcaaggga agaaacatca cggtcatcga cgtcgaagac cccgagttca
    14041 gcggcggcaa gcggctcgga aagacgactt acgaggagct gctagaggag ggcgaccccg
    14101 atttcgcctg gcgctggccc gaggacgagt gggaggcgat ctcgctcaac tacacctcgg
    14161 ggacgaccgg caatccgaag ggcgtggtgt accaccaccg cggcgcctac ctgaacggcg
    14221 tcggcaacat cctggtctgg ggcatgccgc accatgcgat atacctgtgg acgttgccga
    14281 tgttccactg caacggctgg tgctttccct ggaccatggc cgcgaacgcc gggaccagca
    14341 tctgtctgcg gcgggtggaa gcggggccga ttttcgatct catcaagaaa caccgggtga
    14401 cccactactg cggcgcgccc gtcgtccacc agctgctcat caacgccccc gaggagatga
    14461 agcgcggcat cgagcaccag gtgaacggcc tggtcgccgc cgcggcgccc cccgcggcga
    14521 tgatcgaagg catgcagaag atcggcttca acatcaccca cgtctacggc ctgaccgagg
    14581 tgtacggacc cgccacggtg tgcgcgaagc acccggagtg ggagcggctg ccgctcgccg
    14641 accaggtgcg cctgaacggc cggcaaggcg tgcgctacca cgtcgaggaa ggcctgacgg
    14701 tgatggatcc cgagaccatg cagccggtcc cgcccgacgg cgagaccatg ggcgagatca
    14761 tgttccgcgg caacatcacc atgaagggct acctcaaaaa ccccaaggcg acgcgcgacg
    14821 cgttcgccgg cggctggttc cattccggcg acctcgccgt gatgcagccc gacggctaca
    14881 tcaagatcaa ggaccgctcc aaggacatca tcatctccgg cggcgagaac atctcgtcgc
    14941 tcgaggtcga ggacgtgctc taccgccacc ccgcggtgat ggccgccgcc gtggtggcgc
    15001 ggcccgaccc gaagtgggga gaaacgccgt gtgccttcgt ggagctcaag cctggggttg
    15061 ccgtttcaga aactgaaata atcgagttct gccgcggcca gctcgcgaag ttcaaggcgc
    15121 cgcgcgcggt ggtattcggc gagcttccca agacctcgac gggtaagatc cagaaattcg
    15181 ttctcagaga gaaggccaag tccaccgccg ccattgacac atgagcgccg tcccccaagc
    15241 tgagcagccg attctcctgc gcgaagaccg cgacgggatc tgcacgctca ccatgaaccg
    15301 gccgcagcag ctgaacctgc tgacccgcga catgctttcc gctcttcagg atgcctttga
    15361 ttccttgaaa agtgataagc aaacccgggt cgtcgtgctc gccgggaccg gcaaaggctt
    15421 ctgcgccggg cacgacctga aggagatcaa ggcgctgaaa gagctgccga agatcgaggc
    15481 gctgttcggg caatgcagcc ggatgatgca gacgattccg ctgctgccgc agccggtgat
    15541 cgcccgcgtg cagggcgcgg cggcggcggc gggctgccag ctcgtcgcgc agtgcgacct
    15601 cgcaatcgcg tcggacgcag ccaagttcgt caccagcggc gtcacctggg gatttttctg
    15661 ttcgacccca ggcgtcgccg tcggccgcaa cctgcagcgc aagcatgcga tggaaatgct
    15721 cctgaccggc gagccgatca gcgcgactcg ggcaatggag tggggcctgg tgaacaaggt
    15781 cgtgcccttg gatcagctcg atcatgcagt aatggatctt gcaaaaacgc ttgcttccaa
    15841 acccccggcg accgtcgccg cgggcaagcg cgctttctac cagcagatgg accttggcct
    15901 ggaacaggcc tacgcgctcg cttcgagggt catctccgca agcttcgcgc acgaggaagg
    15961 ccgcgccggc atggacgcct tcatcgacaa gcgacctccg ccgaagcact aggtgcgcca
    16021 cgctgcgcat gcccgttgcg tcgcggccct gctggctgcc gcgatccttt g
//
LOCUS       DATBSZ010000042         5955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1508222, whole genome shotgun sequence.
ACCESSION   DATBSZ010000042 DATBSZ010000000
VERSION     DATBSZ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508222"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="VIG70_02835"
     CDS             <1..1496
                     /locus_tag="VIG70_02835"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY8249514.1"
                     /translation="SPGASMTRDEKLRIARALERMKVDVIEAGFPASSNGDFEAVRAI
                     ANTIKDSTVCGLCRANDRDIQRGIDALTGAKSWRIHTFIATSALHMEKKLRMTPEQVF
                     EQAKLSVRFARKACPDVEFSPEDGSRSELDFLCRVLEAVIKEGASTINIPDTVGYAVP
                     QQFGEFIRKLREKIPNSDKAVWSVHCHNDLGMAVANSLAAVQIGGARQIECTINGLGE
                     RAGNTSLEEVVMAVKTRSDFFNLRVGLDTSQIVPTSRLVSQITGFVVQPNKAVVGANA
                     FAHASGIHQDGVLKARQTYEIMRAEDVGWSTNKIVLGKLSGRNAFKQRLKEIGIELDS
                     EDAYNKAFQRFKDLADKKADIFDEDLQALVSQEAGRSSDEHWKLVTMNQASGMGERPF
                     AKVTLMEAGQEKKAESQGDGPVDATFKAIETLAKSGAELLLYSVNAVTTGTESQGEVT
                     VRLAKGGRVVNGIGADTDIVVASAKAYISALNKLESKIERLNPQHQL"
     gene            1493..1915
                     /locus_tag="VIG70_02840"
     CDS             1493..1915
                     /locus_tag="VIG70_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY8249515.1"
                     /translation="MRSLALAALLLALPAHAQMYKCVDERGVTHYTDKPRPDCKGGKV
                     DIRPIPPVSGETAAPPSSFAAQDAEFRRRQIEGAQAEEKEKAALDQRCARLRRELDFL
                     SHGGRISNVDAQGRRVYVDDATRASRLAQVKDQLRLCP"
     gene            1915..2721
                     /locus_tag="VIG70_02845"
     CDS             1915..2721
                     /locus_tag="VIG70_02845"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY8249516.1"
                     /translation="MRRSWLFVPGADRGAHAAAARSGADVIILELEDFTPPELRAKAR
                     ALAGEAFTKWRKAGAIAAVRVNPLETCGRDDLRVVMLARPDVVMMSKVVSADQVRSLA
                     ELAPEAELVPNVETAAGLLNAFHIGKASPRVTAMLVASEDMVADLGTARSRGGGELAY
                     VRARFLVECRAAGVEAIDCPYTFSDAKGAAADARTAKRLGYRMKSLVDPSHARAINAV
                     FTPNEKELAKARKIVAAFERARAEGKERVKVDGALIEVPIYAAAKRLLGG"
     gene            complement(2710..4434)
                     /locus_tag="VIG70_02850"
     CDS             complement(2710..4434)
                     /locus_tag="VIG70_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8249517.1"
                     /translation="MQRKLAAILSADAAGYSRLMSEDEVGTVRILATCRELVSGVIAR
                     HGGRVVDMPGDNILAEFASAVSAVEAASAMQAQLGACNGALPENRRLAFRIGVNLGDV
                     VIEDGRLYGDGVNVAARIEALAEPGGICVSAKIYDEVLRKLDLAFEDLGEHALKNIAA
                     PVRVYRVRPNGPAPARAAAPSQAAKPSIIVLPFTNMSGEAEQEFFADGLTEDILTDLS
                     RFHGLFVISRNTSFKYKGQAVEVRKVARELGVQYVVEGSVRKAGKRVRVTVQLIDAES
                     DRHLWAERYDRDLDDIFALQDEVTNAIVAVLPGRVEAAMHDRAARKPTGNMAAYECVS
                     TAKVLHHRATREDNARAMELIERAIDLDPKYAHGHAWKACIVGQAATYGWLDLAQALV
                     AIERELNIARELDDNDSDVHRIFAALKLRQRDYERSSYHQQRALELNPNDDLIVVQQG
                     EILTWLGQAEEGIEWIRKAMRLNPYHPPRFWLHLARALFAARRYPEAAEALSRIAEAD
                     AFHLAFLAACLAQAGEPARAAEHAQAALRRKPDFSVQKDYVPTLHYKREADLAHHRDA
                     LLKAGLPA"
     gene            complement(4406..4753)
                     /locus_tag="VIG70_02855"
     CDS             complement(4406..4753)
                     /locus_tag="VIG70_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEY8249518.1"
                     /translation="MARASQVVAAAALGALVVLCLAWELWLAPLRPGGSLLALKALPL
                     VLPLSGILKGKRYTYQWSSMLILAYFAEGITRAWSERGSSQALGLIEAVLSLVFFAAA
                     VSYARATQARGNP"
     gene            complement(4732..4917)
                     /locus_tag="VIG70_02860"
     CDS             complement(4732..4917)
                     /locus_tag="VIG70_02860"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HEY8249519.1"
                     /translation="MPQQLRCSFCGKHQDQVKRLVAGAKALICDECVAAADRMMKADP
                     DDPEAAKKPSWWRAPPK"
     gene            complement(4917..>5955)
                     /locus_tag="VIG70_02865"
     CDS             complement(4917..>5955)
                     /locus_tag="VIG70_02865"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8249520.1"
                     /translation="ARRNSVLQWIPRAMTNARVLFCASLILALFGPLMAQAQPAKTWR
                     LGMLTVNPRPALAKSAIYIAFLQELRSLGYREGENLVIEWRHTDGHLDRPRQEAAALM
                     AWKPDVVFTVSGTNAIALRNASASVPIVVGMGGDLVGMRLAATLARPGGNVTGQQLLS
                     PDLAVKRLELLTEVLPKLQRVAMLHAETQVDHGFYDRIFADLQAAAPAVGVTMLRFTA
                     KNGAEVESAFREMAASKVDAVLVIASPFITANRRRILALAAERRIPTMHEVADEADAG
                     GLVAYGPKAEAMFRRGAHYVDRVLKGARPAEMPIEQPTQFELVINRRSANALGVKIPA
                     SILVRADRVIE"
BASE COUNT          982 a         2030 c         2039 g          904 t
ORIGIN      
        1 agagccccgg cgcttcgatg acgcgcgacg agaagctgcg catcgcgcgc gcgctggagc
       61 gcatgaaggt cgacgtgatc gaggccggct tcccggcctc gtcgaacggc gatttcgagg
      121 cggtgcgcgc gatcgccaac acgatcaagg actccacggt gtgcgggctg tgccgcgcca
      181 acgaccggga catccagcgc ggcatcgacg cgctgacggg cgcgaagtcg tggcgcatcc
      241 acaccttcat cgccaccagc gcgctgcaca tggagaagaa gctgcgcatg acgcccgagc
      301 aggtgttcga gcaggcgaag ctctcggtgc gcttcgcccg caaggcgtgc cccgacgtcg
      361 agttctcccc cgaggacggc tcgcgctccg agctcgactt cctgtgccgc gtgctcgagg
      421 cggtgatcaa ggagggggcg agcaccatca acatcccgga taccgtcggc tacgccgtgc
      481 cgcagcagtt cggagaattc atccgcaagc tgcgcgagaa gatcccgaac tccgacaagg
      541 cggtgtggag cgtgcactgc cacaacgacc tcggcatggc ggtggccaac tcgctcgccg
      601 cggtgcagat cggcggcgcg cgccagatcg agtgcaccat caacggcctc ggcgagcgcg
      661 ccggcaacac ttccctcgag gaggtggtca tggccgtcaa gacgagatcg gacttcttca
      721 acctccgggt tgggctcgac accagccaga tcgtgccgac ttcgcgcctc gtgtcgcaga
      781 tcacgggctt cgtagtgcag ccgaacaaag cggtggtggg cgcgaacgcc ttcgcgcacg
      841 cctcgggaat ccaccaggac ggcgtgctga aggcgcgcca gacctacgag atcatgcgcg
      901 ccgaggacgt gggctggagc accaacaaga tcgtgctggg caagctgtcg ggcaggaacg
      961 cgttcaagca gcgcctgaag gagatcggca tcgagctcga cagcgaggac gcgtacaaca
     1021 aggcgttcca gcgcttcaag gacctcgccg acaagaaggc cgacatcttc gacgaggacc
     1081 tgcaggcgct ggtgtcgcag gaggccgggc gctcgagcga cgagcactgg aagctcgtca
     1141 ccatgaacca ggcgagcggc atgggagaac ggccttttgc aaaagtcact ttgatggagg
     1201 cgggccagga gaaaaaagcg gaatcgcagg gcgacgggcc tgtggacgcc accttcaagg
     1261 cgatcgagac cctggcgaag agcggcgccg agctgctcct ctattcggtc aacgcggtga
     1321 cgaccggcac cgagtcgcag ggcgaggtga cggtacggct ggcgaagggc gggcgggtgg
     1381 tgaacggcat cggcgccgac accgacatcg tggtcgcctc ggccaaggcc tacatcagcg
     1441 cgctgaacaa gctcgagagc aagatcgaaa ggctgaaccc ccagcaccaa ttgtgagaag
     1501 cctcgcgctc gctgcgctcc ttctggcgct ccccgcccac gcccagatgt acaagtgcgt
     1561 cgacgagcgc ggcgtcacgc attacaccga caagccgcgc ccggactgca agggcggcaa
     1621 ggtcgacatc cggccgatcc cgccggtctc gggcgagacc gcggcgccgc cgagcagctt
     1681 cgcggcgcag gacgccgagt tcaggcgccg ccagatcgag ggcgcgcagg cggaggaaaa
     1741 agaaaaggcc gcgctcgacc agcgctgcgc ccggctgcgc cgggagctcg actttctgtc
     1801 ccacggcggg cgcatctcga acgtggatgc ccaggggagg cgggtctatg tcgacgacgc
     1861 cacccgggct tcgcgcctcg cgcaggtcaa ggaccagctg cgcctctgcc cttgatgcgg
     1921 cgctcatggc tgttcgttcc gggcgccgat aggggcgcgc acgcggccgc cgctcgcagc
     1981 ggggccgacg tcatcattct ggagctcgag gacttcacgc cgccggagct tcgcgcgaag
     2041 gcgcgcgcgc tcgccggcga agcattcacg aaatggagaa aagccggcgc gatcgcggcc
     2101 gttcgcgtca acccgctcga gacctgcggc cgcgacgatc tgcgggtcgt gatgctggcg
     2161 cgcccggacg tggtcatgat gtccaaggtg gtgtcggccg accaagtgcg gtcgctggcg
     2221 gagctcgcgc ccgaggccga gctcgtgccc aacgtcgaaa ccgctgccgg cctcctgaac
     2281 gccttccaca tcggcaaggc gagcccgcgc gtgaccgcga tgctggtggc gagcgaggac
     2341 atggtcgccg acctcggcac cgcgcgcagc cggggcggcg gggagctcgc ctacgtgcgc
     2401 gcgcgcttcc tggtcgagtg ccgcgcggcc ggagtcgaag cgatcgattg cccctacact
     2461 ttcagcgacg ccaagggcgc ggccgccgac gcgcgcaccg cgaagcggct cggctaccgc
     2521 atgaagagcc tggtcgaccc ttcgcacgcc cgcgccatca acgccgtttt cacgccgaac
     2581 gagaaggaat tggccaaggc taggaaaatc gtcgccgcct tcgaaagagc gcgcgccgag
     2641 ggcaaggagc gcgtcaaagt cgacggcgcg ctgatcgaag tgccgatcta cgcggccgcc
     2701 aagcgcctgc taggcgggta gcccggcttt cagcagggcg tcacgatggt gcgcgaggtc
     2761 ggcttcgcgc ttgtagtgca gcgtcggcac gtagtccttc tgcacgctga aatcgggctt
     2821 gcgccttagc gccgcctgcg cgtgctcggc agcgcgtgcg ggctcgcccg cctgcgcgag
     2881 gcaagcggcg aggaacgcaa ggtgaaaggc atcggcctcg gcgatgcgcg acagcgcctc
     2941 ggcggcctcg gggtagcgcc gcgccgcgaa cagcgcgcgc gccagatgca gccagaagcg
     3001 cggcggatgg tacgggttga ggcgcatggc cttgcggatc cactcgatgc cttcctcggc
     3061 ctggccgagc caggtcagga tctcgccctg ctgcaccacg atcaggtcgt cgttgggatt
     3121 gagctcgagc gcgcgctgct ggtggtagct cgagcgctcg tagtcgcgct ggcgcagctt
     3181 cagggcggcg aagatgcggt gcacgtcgct gtcgttgtcg tcgagctcgc gcgcgatatt
     3241 gagctcccgc tcgatggcca ccagggcttg cgccaggtcg agccagccgt aggtcgccgc
     3301 ctggccgacg atgcaggcct tccacgcatg cccgtgcgcg tactttgggt ccaggtcgat
     3361 ggcgcgctcg atcagctcca tcgcgcgcgc gttgtcctcg cgcgtcgcgc ggtggtgcag
     3421 gaccttggcg gtggagacgc actcataggc agccatgttc ccggtcggct tgcgcgccgc
     3481 gcggtcgtgc atcgccgcct cgacgcggcc gggcagcacc gccacgatgg cgttcgtaac
     3541 ctcgtcttgc agggcgaaga tgtcgtcgag gtcgcggtcg tagcgctccg cccagaggtg
     3601 ccggtcggat tcggcgtcga tcagctgcac ggtcacccgc acccgcttgc ctgccttgcg
     3661 cacgctgccc tcgacgacgt attgcacgcc gagctcgcgc gccaccttcc gcacttcgac
     3721 cgcctggccc ttgtacttga aggaggtgtt gcgcgagatg acgaacaggc cgtggaagcg
     3781 cgacagatcg gtcaggatgt cctcggtcag gccgtcggcg aagaattcct gctcggcttc
     3841 gccgctcatg ttggtgaagg gaagcacgat gatcgagggc ttggccgcct gtgagggcgc
     3901 cgccgcccgc gccggcgccg ggccattcgg ccgcacgcgg tacacgcgca ccggcgcggc
     3961 gatgttcttg agcgcatgct cgccgaggtc ctcgaaagcg aggtcgagct tgcgcagcac
     4021 ctcgtcgtag atcttggccg agacgcagat cccgcccggc tcggcgagcg cctcgatgcg
     4081 cgcggcgacg ttcaccccgt cgccgtagag ccgcccgtcc tcgatcacca cgtcgccgag
     4141 gttcacgccg atgcggaacg cgaggcgccg gttctccggc agcgcgccgt tgcacgcgcc
     4201 gagctgcgcc tgcatggcgc tcgccgcctc caccgcgctc accgcgctcg cgaactcggc
     4261 gaggatgttg tcgccgggca tgtcgaccac gcgcccgccg tgccgcgcga tcacgccgct
     4321 gaccagctcg cggcaggtgg cgaggatgcg cacggtgccg acctcgtcct cgctcatcag
     4381 gcggctgtag cccgccgcgt cggcgctaag gattgccgcg agcttgcgtt gcacgcgcgt
     4441 agcttaccgc agcggcgaag aacacgaggc tcagcactgc ttcaatcaat ccgagggctt
     4501 gagaacttcc cctctcggac cacgcgcgcg ttatgccctc ggcgaaatag gcgaggatca
     4561 gcatgctcga ccactgatag gtgtagcgct ttcccttgag gattccgctc aacgggagaa
     4621 cgagcggaag cgccttgagc gcgagcagcg atccgccggg tcgcagcggc gccagccaca
     4681 gctcccacgc caggcacagg acgaccagcg cgccgagcgc ggccgccgcg actacttggg
     4741 aggcgcgcgc caccaggacg gcttcttcgc agcctcgggg tcgtcgggat ccgccttcat
     4801 catgcggtcg gcggccgcca cgcactcgtc gcagatgagg gccttcgcgc cggcgaccag
     4861 tcgcttcacc tgatcctggt gcttgccgca gaaggagcag cgcagttgct gcggcactat
     4921 tcgatcaccc gatcggcgcg caccaggatc gacgccggaa tcttgacgcc gagcgcgttc
     4981 gcgctcctgc ggttgatcac cagctcgaac tgggtcggct gctcgatcgg catctccgca
     5041 ggccgagcac ccttgagcac gcggtccacg tagtgcgcgc cgcggcggaa catcgcttcc
     5101 gccttcgggc catacgcgac caggccgccg gcgtcggcct cgtcggcgac ttcgtgcatg
     5161 gttggaatgc gccgctctgc cgcaagcgcc agaatgcgcc gccggttcgc cgtaatgaac
     5221 gggctggcga tgaccagaac cgcatcgacc ttgctggccg ccatctcgcg aaaggcgctc
     5281 tccacctcgg cgccgttctt ggcagtgaag cgcagcatcg tcactccgac cgccggcgcg
     5341 gccgcctgca gatccgcgaa gatccggtca tagaagccgt gatccacctg cgtctccgcg
     5401 tgcagcatgg cgacgcgttg cagcttcggc agcacctccg tcaggagctc cagccgcttg
     5461 accgccaggt cgggcgaaag gagctgctgt cccgtgacgt tgccgccggg ccgcgccagc
     5521 gtcgcggcca ggcgcatgcc gacgaggtcg ccgcccatgc cgacgacgat cggcacgctt
     5581 gcgctcgcgt tgcgcagcgc gatggcgttc gtcccgctca ccgtgaaaac cacgtcgggc
     5641 ttccatgcca tcagcgccgc agcctcctgg cgcggccgat ccaggtgtcc atccgtatgg
     5701 cgccattcga tcaccaggtt ttctccctcc cggtagccga ggctgcgcaa ctcctgcagg
     5761 aacgcaatgt agatggcgct cttggcaagc gccggacggg ggttcacggt cagcatgccg
     5821 aggcgccagg tctttgcagg ctgcgcctgc gccatgagcg gtccgaacag cgcgaggatc
     5881 aggctggcgc aaaaaaggac gcgcgcattg gtcatcgctc ttggtatcca ctgcaacacg
     5941 ctgttgcgtc ttgcg
//
LOCUS       DATBSZ010000043        16400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2191176, whole genome shotgun sequence.
ACCESSION   DATBSZ010000043 DATBSZ010000000
VERSION     DATBSZ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16400
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2191176"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VIG70_02870"
     CDS             <1..219
                     /locus_tag="VIG70_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249521.1"
                     /translation="CVNPTMLTGLKERLQAIDAETRATFIVIEHNMEFVMSLCRRILV
                     LAEGRIIAEGTPEAVRSNPQVIEAYLGH"
     gene            209..994
                     /locus_tag="VIG70_02875"
     CDS             209..994
                     /locus_tag="VIG70_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249522.1"
                     /translation="MATETRDDGCILVVDGLVAGYGNMTILNETSARIRRGAVTTVIG
                     PNGAGKSTLFNVLAGVIEPDSGSVAFEGRSLLGLRPDQRARAGLVRTFQISRELRGLT
                     VLENVLLASPGQSGDAAWRCFATPRRVRAEQEAAIARARKLLEQVDLWPLANEPAGNL
                     SGGQKKLLEISRALMQEPEIILLDEPAAGVSPLMIKVLGETILRLRAQGITFAIIEHD
                     MDVIAELCEPIYVLAEGRTLTHGTFREVASNREVMQAYLGKVA"
     gene            991..1743
                     /locus_tag="VIG70_02880"
     CDS             991..1743
                     /locus_tag="VIG70_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249523.1"
                     /translation="MSAVLSVAGLHAGYGAGDILKGIDVSLDEGRILTIIGPNGSGKS
                     TLVKTLAGLLPARQGDVRLAGERVNELSPPKRVRRGLAYVPQEYNVFRNLTVLENLRI
                     AHEFMGGGAGAPLPEEMAALFPELPSRYPALAGNLSGGQRQMLAFACALTVRPKVLML
                     DEPSAGLSPLLTVEIFRTVKNVNAMGVSVLMVEQNAIEALRISDACVVLAGGRVRLAA
                     PAAEVLGMKDLQRLYLGDAQQANGQPAGRAAA"
     gene            1743..2603
                     /locus_tag="VIG70_02885"
     CDS             1743..2603
                     /locus_tag="VIG70_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8249524.1"
                     /translation="MLQTAFNGLVTGVIMTLPALAVTLLFGVLKFPNFAVGAMMTVAA
                     YMVFALNAQLGWPLAPATAVVAVAFGGLCIAIDQLTFKPLRGRGGITLMVASLGLGFV
                     LENIARFAYGNTARSFAIELARPFRFLDVRMNREQMITLALSALAMTLMWLLLTRTPV
                     GRAMRAVADNPQLALVRGIDSERIIRWTWFITGILLAVGGVLIGMDRALEPPMGSNYV
                     VGVFGAAILGGLGSALGAFAGAMIIGVVSELSTLVMPPNYRIGIPLAAIALVLVFRPQ
                     GLFGQPYIRK"
     gene            2611..3516
                     /locus_tag="VIG70_02890"
     CDS             2611..3516
                     /locus_tag="VIG70_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8249525.1"
                     /translation="MISFLVLLGIVVCFSAILALALNFQWGLGGMVNFGLAGFYTLGA
                     YSCALLILNGGANTLAATLGAMAIVAAACAATALVTLRVTEEDYFAIVTLGVGEMLRL
                     VALNEDWLTKGALGLTGIPRPLGDVIPADVYVYFLLGLSIALLLGTLWVLNVLARSPF
                     GRVVRALREDDVVAATLGKNVLWARVRIFAIGGAFIGLAGSMHAFYYQYIDPTQFTNI
                     VIAYAFMAVIAGGRGAHAGAIWGAAAVMVLLEGSRFLKDVIPVLDSDRLAAIRIIIIG
                     VGLILLLIYRPQGFMREYRLQVELK"
     gene            3569..4795
                     /locus_tag="VIG70_02895"
     CDS             3569..4795
                     /locus_tag="VIG70_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8249526.1"
                     /translation="MNKTRRDVIKSIGAATASSIAAPYLNLAHAQGAPIPIGVGLPLT
                     GNAGAYGPDMGEAAKRTAAKINAAGGILGGRKLELFIEDSESSASVAANLTQKFINVH
                     KCQSCIGYWGTPEGMSSRPIVVQNKVVLMVSSAANAITEGDTQGYVWRFQMKATDWGI
                     VIGRAAVRQGFKTVGILGLQNAFVLPIMKVFEEQMKAAGRQVVDSLIYNPEQPSYRAE
                     VERIFGKKPEAVYCFGLLPDFVSIMKEVYRGGFDSKVVALSIMADAEGKFVQAVGNQV
                     AEGIRHFQPMPDVSASGYKKFLQLMGAPADRVFLFPPNTHDQLAITALAMERAKSPMA
                     VDWSKQIITVGNGPGPEVDDVVEALKLVRAGKPVNFQGAGSTCDFTPNGDQLGRGMGQ
                     WIIKGGKSVFVEYAKP"
     gene            4798..5931
                     /locus_tag="VIG70_02900"
     CDS             4798..5931
                     /locus_tag="VIG70_02900"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8249527.1"
                     /translation="MLAGLRVLEFGQIAAGPFAGSLLADLGADVVKVEKPDGGDDMRR
                     WPPVSDGYSENFASINRNKRSIAVDLKDAAQVGRLKRLCGKADAIIENYRPGVLERLG
                     LGYEALRAASPRLVYCSINGYGHAGPYAGKGAFDATIQGISGVMSVTGEPGGPPVKCG
                     VPLGDFGAGLYGAFCITSAILRARETGEGAFIDCSMLAGLLGMAALQTSEYFGTGRSP
                     QRLGSAHPRAAPYQGFQASDAPFMIAAGNDKLWMAVCDAIGVPQLKTDPRFETNALRA
                     KNQKRLSEILQREFSKRSKAEWLEELDRRGVPCAPINTYAEVLDDPHVRATRLVRDMT
                     LPNGRKTRTIAFPMKISGYEFSVYRDPPLLGEHNDEVFGEWLE"
     gene            5919..6455
                     /locus_tag="VIG70_02905"
     CDS             5919..6455
                     /locus_tag="VIG70_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY8249528.1"
                     /translation="MARVRDVGPEELPPQVREVYERFAGGYGAFRDQVGVFAHVPSSV
                     AHLMAMLLELREQKNVPYRYVELAVVAVSKLNECHYCVGQHKPLLAIEGISPGGAERV
                     LDYGTNPELDEVDRLVVEYSIAVTNAPQRIHDEIFERLRRHFSEAQIVELTLRIALCG
                     FFNRFNDALQIEREPHDH"
     gene            6445..6846
                     /locus_tag="VIG70_02910"
     CDS             6445..6846
                     /locus_tag="VIG70_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8249529.1"
                     /translation="MITKGIHQLVAEAEKRSKGISVEEAKKRVGDANTVFVDIRDVRE
                     LEREGMIPGAFHAPRGMLEFWVDPESPYYKPVFTDDKTFILYCQADWRGVLAAATLAE
                     MGMTNVLHLNGGFGEWRKAGGPTGPKPEKKK"
     gene            complement(6869..7330)
                     /locus_tag="VIG70_02915"
     CDS             complement(6869..7330)
                     /locus_tag="VIG70_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEY8249530.1"
                     /translation="MKTGLRFKAWVAAVLAVVFVGGCANSLGGSSFAYSYEPQFSFAE
                     LKTYRWTEARPHYWGDPLLESNVRFLADRVLQTKGFVSSADKSDFIVSMRYEGSYVYE
                     LRGLTLSVSRADNSGVIWRGAATGVIRTDASSGDLKNAVEGILSNFPPRQK"
     gene            7433..8344
                     /locus_tag="VIG70_02920"
     CDS             7433..8344
                     /locus_tag="VIG70_02920"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8249531.1"
                     /translation="MADRRLQVFHAVAKHLSFTKAAETLFMTQPAVTFQIKQLEEHLS
                     TRLFDRAQGRIILTPAGQVALEYAERILALSAELDTRLKEMGGHAAGPLLIGASMTIG
                     EYVLPQLIGKFKAQFPAVVPALFVGNSEAVQERVDERTLDLGFIEGDSHLGSLHSELC
                     CEDELQVVCAPSHPLAREGFALPSALTQHPYINREPGSGTRAVIDRYLQKAGVAPESL
                     NVVVELGSPEAIKGLVETGLGFAIMSRAIVAKELQLRLLAQIPLQPRLIRNFSVVYPK
                     ERFHSKLVAAFLAFAKEQLAAAQRLAA"
     gene            complement(8326..9483)
                     /gene="sat"
                     /locus_tag="VIG70_02925"
     CDS             complement(8326..9483)
                     /gene="sat"
                     /locus_tag="VIG70_02925"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEY8249532.1"
                     /translation="MPLVTPHGGGGLKPLALTGKALAAETARARTLPALRISSREKGD
                     LVMLGIGGFTPLDGFMTRADWEGVCERYRTASGLFWPIPITLSSQESFPGGSEIALTD
                     PDGGAPLATMKVVETYKIDKNQECSSVFRTTDPAHPGVAMVMAQPEFNLAGPVKVLSD
                     GGFKAKYGTLFMTPAETRAEFTRLGWSRVAAFQTRNPMQRAHEYLAKVAIEVCDGVLV
                     HSLLGNLKAGDIPADVRTRAIAALIENYFRPGTVVQAGYPLDMRYAGPREALLHALFR
                     QNYGCSHLLVGRDHAGVGSYYGPFDAHRIFDEIPAGSLETRPLKIDLTFWCYRCHGMA
                     SGRTCPHGVKDQLQVSGTQLRKWLEEGAEVPPEFSRPEVLAILREYYAARR"
     gene            complement(9557..10447)
                     /locus_tag="VIG70_02930"
     CDS             complement(9557..10447)
                     /locus_tag="VIG70_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249533.1"
                     /translation="MKLRFAIVLLGLISQPALAQWRVSSDQAVGGFVFPESVAYDAKG
                     KALYVGNFGGQKLAPAEKDGLGYISKVGLDGKVLEQKWVPAQGGEPLHKPKGIWIRGE
                     RLWVTDIDVVWIFDLKTKKGRKLALPGVGFANDPAVMGNALYISDNRNDKLVKVEPAD
                     FLNAKDPKVTEVLAGLGHNPNGVYPTKVGMLLFGGFLAPDKPKGLYALGVSGQVRQIS
                     GPIGRIDGLYEMADGSLLYTDWNTGSLSHWSEKGGVRKLADGFKGPADFTVIPGAGGS
                     MTVVVPDLVQSQLRFIQLRK"
     gene            10562..11422
                     /locus_tag="VIG70_02935"
     CDS             10562..11422
                     /locus_tag="VIG70_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8249534.1"
                     /translation="MRTVALTIALAGALHAGVSLAQRRPATEAEAMSYITSAFISQAD
                     PAAMSPGVALGPELEKALGLQPGTDRVKVYQAVVPLADDPRLEVRRAAPADVADYGAR
                     RGFDPASPHPLFTVQTAKVKLLVQYDLKAFNIVFVGQLGMPDPDPVVVAKADPAAPAP
                     SQPALVNFAWTAEFSYGSAELTPEARARLDGEIVPKLIASKEIRYLNVYGHSDRTGSA
                     EYNRRLSEKRAEAVRDYLVSKGVDPDKIEVFGYGQTLPVKSCRDEKERALIECLAPNR
                     RIVVEIQATP"
     gene            complement(11431..12918)
                     /gene="glpK"
                     /locus_tag="VIG70_02940"
     CDS             complement(11431..12918)
                     /gene="glpK"
                     /locus_tag="VIG70_02940"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY8249535.1"
                     /translation="MRTILALDQGTTSSRAILFDEEGRPVSTAQREFRQIFPQPGWVE
                     HDPKEIFSTQRETARQAVSQSGIPLKNLMAVGITNQRETTIVWDRQTGEPIANAIVWQ
                     DRRTAPQCAELKQIGAEGLVRERTGLVIDPYFSGTKIAWLLDNVPGARKRAELGELAF
                     GTVDTWLTWHLTGNRTHVTDVSNASRTMLFNIHMNDWDPELLRLLDVPRAILPDVHPS
                     AHAFGMLPAGVLGEPLVIGGVAGDQQAALFGQACHRAGMAKNTYGTGCFMLLHTGDKV
                     VQSSHGLISTACAQTRGKEFALEGSVFIGGAVVQWLRDGLEFFSSSAEIEKLAASVLD
                     SGDVYVVPAFTGLGAPYWDPDARGTIVGLTRGTTKAHIARAALESIAFQSAELLEAMQ
                     KDAGEKLSELRVDGGAAANDLLMQFQADLLGVPVVRPKVLETTALGAAYLAGLTVDLW
                     KSRDELASHWKAERRFEPRMSASDSEARMTRWREAVGRSRRWATP"
     gene            12956..13522
                     /locus_tag="VIG70_02945"
     CDS             12956..13522
                     /locus_tag="VIG70_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEY8249536.1"
                     /translation="MPLPVTDVERELRHTRRVRFEGYKRADGLWDLEAHLTDIKSHDN
                     PMKTGVRRAGQPIHEMWIRLTIDRHLTILDASASSDAVPYPGGCETIAPAYKRLIGLN
                     LVRDFRRAVKKHFGDVKGCTHLTEMLAGFPTAAIQTFAGEMPEERTDGRKPFQLDQCH
                     ALDTAGETVKKWYPKWYSGKSGKEKAPA"
     gene            13523..14686
                     /gene="sucC"
                     /locus_tag="VIG70_02950"
                     /pseudo
     CDS             13523..14686
                     /gene="sucC"
                     /locus_tag="VIG70_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
     gene            14934..15812
                     /gene="sucD"
                     /locus_tag="VIG70_02955"
     CDS             14934..15812
                     /gene="sucD"
                     /locus_tag="VIG70_02955"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY8249537.1"
                     /translation="MSILIDKNTRVMTQGITGKTGMFHTQTSRAYANGKACFVAGVNP
                     KKAGENFEGIPIFGSVLEARKTTGATVSVIYVPPPFAAAAIEEAVDADLDLVICITEG
                     IPVRDMIRTKDRMKGRRTLLVGPNCPGVITPDEIKIGIMPGHIHKKGPIGVVSRSGTL
                     TYEAVGQLSELGLGQSSCVGIGGDPVNGVKHIDVLRMFNEDPKTEAVIMVGEIGGSDE
                     ENAAQWVKKNMKKPVVGFIAGITAPPGKRMGHAGAIISGGKGTAQEKLAVMEACGIKV
                     TRNPAEMGKTLKSVLR"
     gene            15844..>16400
                     /locus_tag="VIG70_02960"
     CDS             15844..>16400
                     /locus_tag="VIG70_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249538.1"
                     /translation="MVATLPYAATIFLSSFLLFLVQPIIAKQILPWFGGSAGVWTTCL
                     VFFQSVLLAGYAYADWTTRLGARRQAMLHVALLAASLLCLPILASTGWKPQGDEQPIL
                     RILLLLGATIGLPYFLLSTTTPLLQAWYWRRFRSVVPYRLFALSNLASLLALLGFPVL
                     FEPAFDLTSLGWGWSFLFAGFAILCA"
BASE COUNT         2835 a         5377 c         5476 g         2712 t
ORIGIN      
        1 tgcgtcaacc cgaccatgct gacgggcctc aaggagcggc tgcaggcgat cgacgcggag
       61 accagagcga cgttcatcgt catcgagcac aacatggagt tcgtcatgtc gctgtgccgg
      121 cgcatcctgg tgctggccga aggacggatc atcgccgagg gcacgcccga ggccgtgcgc
      181 agcaaccccc aggtgatcga ggcgtacctt ggccactgag acgcgcgacg acggctgcat
      241 cctggtggtc gacggcctcg tcgccggcta tggcaacatg accatcctca acgagacgtc
      301 ggcccgcata cgccgcggcg ccgtcaccac cgtgatcggg ccgaacggcg ccggcaagtc
      361 gacgctgttc aacgtgctgg cgggcgtgat cgagccggac tccggcagcg tcgctttcga
      421 aggccgcagc ctgctcggct tgcgcccgga ccagcgcgcg cgcgcgggac tcgtgcgcac
      481 cttccagatt tcgcgcgagc tgcgcgggct tacagtgctc gagaacgtgc tgctcgcaag
      541 tcccggccag agcggcgacg cggcgtggcg ctgcttcgcg acgccgcgcc gcgtgcgcgc
      601 ggagcaggaa gcggcgatcg ctcgcgcgcg caaattgctc gagcaggtgg acctgtggcc
      661 gctggcgaac gagcccgccg gcaatctgtc gggcggccag aagaagctgc tcgagatttc
      721 gcgcgcgctg atgcaggagc ccgagatcat cctgctcgac gagcccgcgg ccggggtcag
      781 cccgctgatg atcaaggtgc tcggcgagac gatcctgagg ctgcgcgcgc agggcatcac
      841 gttcgcgatc atcgagcacg acatggacgt gatcgccgag ctgtgcgagc cgatctacgt
      901 gctcgccgag ggccgcacgc tgacccacgg cacgttccgc gaagtggcct ccaaccggga
      961 agtcatgcag gcgtacctgg gcaaggtcgc atgagcgcgg tgctgtcggt cgccgggctg
     1021 cacgccggct acggcgccgg ggacatcctg aaagggatcg acgtatcgct ggacgaaggc
     1081 cgcattctca ccatcatcgg gccgaacggc tccggcaagt cgaccctggt gaagacgctt
     1141 gccggcctgc tccccgcgcg ccagggcgac gtgcggctcg ccggcgagcg cgtgaacgag
     1201 ctgtcgccgc cgaaacgggt gcggcgcggt ctcgcctacg tgccacagga gtacaacgtg
     1261 tttcgcaacc tcacggtgct ggagaacctg cgcatcgcgc acgagttcat gggcggcggc
     1321 gcgggcgcgc cgctgccgga ggagatggcg gcgctgttcc cggagctgcc gtcgcgctat
     1381 ccggcgcttg ccggaaacct gagcggcggc cagcgccaga tgctcgcttt cgcctgcgcg
     1441 ctcacggttc gtccgaaggt gctgatgctg gacgagcctt ccgcggggct ttcgcccctg
     1501 ctcaccgtcg agatcttccg cacggtgaag aacgtgaatg cgatgggcgt gagcgtgctg
     1561 atggtcgagc agaacgcgat cgaggcgctg cgcatctcgg acgcctgcgt ggtgctggcc
     1621 ggcgggcgcg tgcgccttgc cgcgccggcg gccgaggtgc tcggcatgaa ggacctgcag
     1681 cgcctgtacc tcggcgacgc gcagcaggcc aatgggcaac cagcggggag agcggcggcc
     1741 tgatgctgca gaccgcgttc aacggcctgg tcaccggcgt catcatgacg ctgccggcgc
     1801 tcgccgtgac gctgctgttc ggcgtgctca agttcccgaa tttcgccgtc ggcgcgatga
     1861 tgacggtggc cgcctacatg gtgtttgcgc tgaacgcgca gctcggctgg ccgctggcgc
     1921 cggcgaccgc ggtggtcgcg gtcgccttcg gcggcctgtg catcgccatc gaccagctca
     1981 ccttcaagcc gctgcgcggt cgcggcggca tcacgctgat ggtcgcctcg ctcggcctgg
     2041 gtttcgtcct cgagaacatc gcgcgcttcg cctacggcaa cacggcgcgc agcttcgcca
     2101 tcgagctggc gcgaccgttc cgtttcctcg acgtgcgcat gaaccgcgag cagatgatca
     2161 cgctcgcgct atcggcgctg gcgatgacgc tgatgtggct gctgctcacg cgcacgccgg
     2221 tcggccgcgc catgcgcgcg gtcgcggaca atccgcagct cgcgctggtg cgcggcatag
     2281 acagcgagcg catcatccgc tggacctggt tcattaccgg catcctgctc gcggtgggcg
     2341 gcgtgctgat cggcatggac cgcgcgctgg agccgccgat gggctccaac tacgtggtcg
     2401 gcgtgttcgg cgccgcgatc ctcggcggac tgggcagcgc gctcggcgcc tttgccggcg
     2461 cgatgatcat cggcgtggtg agcgagctgt cgacattggt catgccgccc aactaccgca
     2521 tcggcatccc gctcgcggcg atcgcgctcg tcctggtgtt caggccgcag ggcctgttcg
     2581 ggcaacccta tatacgaaaa tgagccgaaa atgatttcgt tcctggtcct gctcggcatc
     2641 gtggtctgct tctcggcgat ccttgcgctg gcgctcaact tccagtgggg cctcggcggc
     2701 atggtgaatt tcggcctcgc cggcttctac acgctcggcg cctacagctg cgcgctgctg
     2761 attctcaacg gcggcgcgaa cacgctggcg gcgacgctgg gcgcgatggc gatcgttgcc
     2821 gcggcctgcg ccgccacggc gctcgtcacg cttcgcgtga ccgaggagga ttacttcgcc
     2881 atcgtcacgc ttggggtcgg cgagatgctg cgcctcgtag cgctgaacga ggactggctg
     2941 accaagggcg cgctcggcct gacgggcatt ccgcgtccgc tcggcgacgt catcccggcg
     3001 gacgtgtacg tttacttcct cctcggcctg tcgatcgcgc tgctgctcgg cacgctgtgg
     3061 gtgctcaatg tcctcgcgcg ttcgcccttc ggccgcgtgg tgcgcgcgct gcgcgaggac
     3121 gacgtcgtcg ccgccaccct gggaaagaac gttctctggg cgcgggtgcg cattttcgcg
     3181 atcggcggcg cgttcatcgg gctggcagga tcgatgcatg ctttctacta ccagtacatc
     3241 gacccgacgc agttcaccaa catcgtcatc gcctacgcct tcatggcggt catcgcgggc
     3301 gggcgcggcg cgcacgccgg ggcgatctgg ggcgcagccg cggtcatggt gctgctggaa
     3361 ggctcccgct tcctgaagga tgtgatcccg gtgctcgact ccgaccggct tgccgccatc
     3421 cgcatcatca tcatcggcgt cggcctgatc ctgctgctga tctaccggcc gcagggcttc
     3481 atgcgcgagt accgcctgca ggtggaattg aagtagtctc gatctctaga gtacgcggag
     3541 aaaccgctta caaattcgga ggagacagat gaacaagacc cgtcgtgacg taatcaagtc
     3601 catcggcgcc gccacggcgt ccagcatcgc cgccccgtac ctcaacctcg cgcacgcgca
     3661 gggcgcgccg attccgatcg gcgtcgggct gccgctcacc ggcaacgccg gcgcctacgg
     3721 gcccgacatg ggcgaagcgg ccaagcgcac ggcggcgaag atcaacgccg ccggcggcat
     3781 cctcggcggc aggaagctgg agctcttcat cgaggactcc gaatcgagcg cttcggtcgc
     3841 cgccaacctg acgcagaaat tcatcaacgt gcacaagtgc cagtcctgca tcggctactg
     3901 gggaacgccg gagggcatgt cctcgcgccc gatcgtggtg cagaacaagg tcgtgctgat
     3961 ggtctcgagc gcggccaacg ccattaccga gggtgacacc cagggctacg tctggcgctt
     4021 ccagatgaag gcgaccgact ggggcatcgt catcggccgt gccgcggtac gccagggctt
     4081 caagacggtc ggcatcctcg gcctgcagaa cgccttcgtg ctgccgatca tgaaggtgtt
     4141 cgaggagcag atgaaggccg cgggccgcca ggtggtcgat tcgctgatct acaacccgga
     4201 gcagccctcc taccgggccg aggtcgagcg catcttcggc aagaagcccg aggcggtgta
     4261 ctgcttcggc ctgctgcccg acttcgtctc catcatgaag gaggtgtacc gcggcggctt
     4321 cgacagcaag gtggtggcgc tttcgatcat ggccgacgcc gagggcaagt tcgtgcaggc
     4381 ggtgggcaac caggtcgccg agggaatccg ccacttccag ccgatgcccg acgtctccgc
     4441 gtccggctac aagaaattcc tccagttgat gggcgcgccc gccgaccgcg tgttcctgtt
     4501 cccgccgaac acgcacgacc agctcgccat caccgcgctg gcgatggagc gcgcgaaaag
     4561 cccgatggcg gtggactggt cgaagcagat catcacggtc ggcaacggcc cggggccgga
     4621 ggtggacgac gtggtcgaag ccctcaagct cgtgcgcgcg ggcaagccgg tcaacttcca
     4681 gggggcgggc tcgacctgcg atttcacgcc gaacggcgac cagctcggcc gcggcatggg
     4741 ccaatggatc atcaagggcg gcaagagcgt gttcgtcgag tacgcgaagc cctgagcgtg
     4801 ctggcgggcc tgcgggtatt ggaattcggc cagatcgccg cagggccttt tgcgggatcc
     4861 ctgctggcgg atctcggcgc ggacgtggtc aaggtcgaga agcccgacgg cggcgacgac
     4921 atgcggcgct ggccgccggt cagcgacggc tatagtgaga atttcgcctc catcaatcgc
     4981 aataagcgca gcatcgcggt cgatctgaag gatgcagcgc aggtcggccg gctcaagcgc
     5041 ctgtgcggca aggccgacgc catcatcgag aactatcgcc ccggcgtgct cgagcgcctc
     5101 gggctgggct acgaggcgct gcgcgccgct tcaccccgcc tcgtctactg ttcgatcaac
     5161 ggctacggtc atgccggacc ctacgctggc aaaggcgcgt tcgacgctac catccagggc
     5221 atcagcggcg tcatgagcgt caccggagag ccagggggcc caccggtgaa atgcggcgtg
     5281 ccgttgggcg acttcggcgc gggactgtac ggcgcgttct gcatcacttc ggcgatcctg
     5341 cgcgcgcgcg agacgggcga gggcgcgttt atcgactgct cgatgctcgc cgggcttctc
     5401 ggcatggcgg cgctgcagac cagcgagtac ttcggcaccg gaaggtcgcc gcagcggctc
     5461 ggctccgcgc acccgcgcgc cgcgccctac cagggtttcc aggcgagcga cgcgccgttc
     5521 atgatcgccg ccggcaacga caagctctgg atggcagtct gcgacgccat cggcgtgccg
     5581 cagctcaaga ccgacccgag attcgagacc aatgccctgc gcgcgaaaaa ccagaagcgg
     5641 ctcagcgaaa tcctgcagcg cgagttctcg aagcgcagca aagccgagtg gctggaagag
     5701 ctcgaccggc gcggcgttcc ttgcgcgccg atcaacacct atgccgaggt tctggacgac
     5761 ccgcatgtcc gggccacccg cctggtgcgc gacatgacgc tgcccaacgg ccgcaagacc
     5821 aggaccatcg cttttccgat gaagatcagc ggctacgaat tcagcgtgta ccgcgatccg
     5881 ccgctgctgg gcgagcacaa cgacgaggtt ttcggcgagt ggctagagta agggacgtcg
     5941 gcccggaaga actgccgccg caggtgcgcg aggtctacga acgcttcgcg ggcggctacg
     6001 gcgccttccg cgaccaggtt ggcgtgttcg cgcacgttcc ctcatcggtc gcgcacctga
     6061 tggcaatgct gctcgagctg cgcgagcaga agaacgtgcc ttaccgctat gtcgagctcg
     6121 cggtcgtcgc ggtgtcgaag ctgaacgagt gccactactg cgtcggccag cacaagccgc
     6181 tgctcgcgat cgagggaatc tcgcccggcg gcgcggaaag ggtcctcgac tacggaacca
     6241 accccgagct cgacgaggtg gaccggttgg tggtcgagta ttcgatcgcc gtgacgaacg
     6301 cgccgcagcg gatccacgac gaaatattcg aaaggctgcg tcggcatttc agcgaggcgc
     6361 agatcgtcga gctgacgctg cgcatcgcgc tgtgcggatt cttcaaccga ttcaacgacg
     6421 ccctgcagat cgagcgagag ccccatgatc actaaaggca tccatcagct ggtcgccgag
     6481 gcggaaaagc gctcgaaggg aatttcggtc gaggaggcga agaagcgcgt gggcgacgcg
     6541 aacaccgtgt tcgtggacat ccgagacgtg cgggagctcg agcgcgaggg gatgattccg
     6601 ggcgcgtttc acgcaccgcg cggcatgctg gagttctggg tggatccgga gagcccgtac
     6661 tacaagccgg ttttcaccga cgacaagacg ttcatcctct actgccaggc cgactggaga
     6721 ggagtactcg cggctgcaac cctggcggag atggggatga ccaacgtgct gcacctgaac
     6781 ggcgggttcg gcgagtggcg caaggccggc gggccgaccg ggccgaagcc ggagaagaag
     6841 aagtaagaat ccgggtccga gagcgacgct atttttgcct tgggggaaag ttgctcagga
     6901 ttccctcaac cgcattcttc agatcgcccg aagaggcgtc ggtcctgatc acccccgtgg
     6961 ccgccccccg ccaaatcacc ccactgttgt cggcgcggga aaccgacagg gtcagcccgc
     7021 ggagctcata gacatagctg ccctcataac gcatcgaaac gatgaagtct gacttgtccg
     7081 cgctcgaaac aaaccctttg gtctgcagca cacgatccgc caggaatcgg acgttgcttt
     7141 ccagcagcgg gtccccccaa tagtgtggcc tggcttcggt ccaccggtag gtcttcaact
     7201 ccgcgaagct gaattggggt tcgtacgagt aggcgaagga cgagccgccg agggaattgg
     7261 cgcagccgcc aacgaacacc acggccagaa cggcggccac ccaagccttg aaacgcagac
     7321 cagtcttcat ggaagctccc aaccgggaat tcaggcccga acgctagcag agaatgggtc
     7381 atgcaccaag gacgtgaagg gatcaggacc ctttttctcg taatatcgcc ggatggccga
     7441 ccggcgcctc caggtattcc acgcggtagc gaagcacctc tccttcacca aggctgccga
     7501 gacgctgttc atgacccagc cggcggtcac tttccagatc aagcagctcg aagagcacct
     7561 gagcacgcgc ctgttcgacc gcgcgcaggg ccggatcatc ctcacgcccg cggggcaggt
     7621 cgcgctcgag tatgccgagc gcatcctcgc gctctccgcc gagctcgaca cgcggctcaa
     7681 ggaaatgggc ggccacgccg ccggcccgct cctcatcggg gcgagcatga ccatcggcga
     7741 atacgtgctg ccgcagctga tcggcaagtt caaggcgcag tttcccgcgg tggtgccggc
     7801 gctcttcgtc ggcaattccg aggcggtgca ggagcgcgtc gacgagcgca cgctcgacct
     7861 cggcttcatc gagggcgact cgcacctcgg ctcgctgcac agcgagctgt gctgcgagga
     7921 cgagctgcag gtggtgtgcg cgccgtcgca cccgctggcg cgcgaaggct tcgcgctgcc
     7981 ctccgcgctc acgcagcacc cctatatcaa ccgcgagccc ggctccggca cgcgcgcggt
     8041 gatcgaccgc tatctgcaga aggcgggtgt cgcgcccgag tccctcaacg tggtggtcga
     8101 gctcggcagc cccgaagcga tcaagggcct ggtcgaaacc ggcctgggct tcgccatcat
     8161 gtcgcgcgcc atcgttgcga aggagctgca gcttcgcctg ctggcgcaga ttcccctgca
     8221 gccgcggctg atccgcaatt tttccgtggt gtacccgaaa gagcgcttcc actcgaagct
     8281 ggtggcggca ttcctcgcgt tcgcaaaaga gcagctcgca gccgctcagc gcctcgccgc
     8341 gtagtactcc cgcaggatcg ccagcacttc cggccgcgag aattccggcg gcacctcagc
     8401 gccttcctcg agccacttgc gcagctgcgt tccggacacc tgcagctggt ccttcacgcc
     8461 gtgcgggcag gtgcgacccg aagccatgcc gtggcagcgg tagcaccaga aggtcaggtc
     8521 gatcttgagc ggacgcgtct cgagcgaccc ggcggggatc tcgtcgaaga tgcggtgcgc
     8581 gtcgaacggc ccgtagtagc tcccgacccc ggcgtggtcg cgccccacga gcaggtgcga
     8641 gcagccgtag ttctggcgga agagggcgtg cagcagcgcc tcgcgcgggc cggcatagcg
     8701 catgtcgagc ggatagccgg cctgcaccac ggtgcccggg cggaagtagt tttcgatgag
     8761 cgcggcgatc gcccgggtgc gcacgtcggc cggaatgtcg cccgccttga ggttgccgag
     8821 cagcgaatgc accaacacgc cgtcgcacac ctcgatcgcg accttggcga gatattcgtg
     8881 cgcgcgctgc atcgggttgc gcgtctggaa cgccgcgacg cgcgaccagc cgaggcgcgt
     8941 gaattcggcg cgcgtctctg ccggcgtcat gaagagcgtg ccgtacttcg ccttgaaccc
     9001 gccgtcggac agcaccttca ccgggccggc gagattgaac tcgggctgcg ccatcaccat
     9061 cgcgacgccg ggatgcgcgg gatccgtcgt gcggaatacc gagctgcact cctggttctt
     9121 gtcgatcttg taagtttcaa cgaccttcat cgttgcgagc ggagcgccgc cgtcagggtc
     9181 ggtgagcgca atttcgcttc cgccgggaaa gctttcttgc gaagacaagg tgatcgggat
     9241 cggccagaac agccctgaag cggtgcggta gcgctcgcac acgccttccc agtcggcgcg
     9301 cgtcatgaag ccgtcgagcg gcgtgaagcc gccgatgccg agcatcacga ggtcgccttt
     9361 ctcgcgcgag ctgatgcgca acgccggcag cgtgcgcgcg cgagcggtct cggcggcgag
     9421 cgctttcccc gtcagcgcga gcggcttgag gccacctcct ccgtgcggcg tgaccagggg
     9481 cacgaagtta ttttcgggag ccgccgcgga acaggcaaaa ccgctgtact gatccggcga
     9541 taaggctccc tgatagctac ttgcgcagct gaatgaagcg cagctggctc tgcaccagat
     9601 ccggcaccac gacggtcatg ctgccgcccg cgcccgggat cacggtgaag tcggccgggc
     9661 ccttgaagcc gtcggcgagc ttgcggacgc cgcctttctc gctccagtgc gagagcgagc
     9721 cggtattcca gtcggtgtag aggagcgagc cgtcggccat ctcgtagaga ccgtcgatgc
     9781 ggccgatcgg gcccgagatc tgcctgacct ggccgctgac gccgagcgcg tacaggccct
     9841 tcggcttgtc gggggcgagg aagccgccga agagcagcat gccgaccttg gtcgggtaga
     9901 cgccgttcgg gttgtggccc aggcctgcga ggacttcggt cactttcgga tccttggcat
     9961 tcaggaaatc ggcgggctcc accttcacca gcttgtcgtt gcggttgtcg ctgatgtaga
    10021 gcgcgttgcc catgaccgcc gggtcgttgg cgaagccgac gccgggcagg gcgagcttgc
    10081 ggcccttttt cgtcttcaga tcgaaaatcc acaccacgtc gatgtcggta acccacaggc
    10141 gctcgccgcg gatccagatg cctttcggct tgtgaagcgg ctcgccaccc tgcgccggga
    10201 cccacttctg ctcgagcacc ttgccgtcca ggccgacctt gctgatgtag cccaagccgt
    10261 ccttctccgc cggggcgagc ttttgcccgc cgaagttgcc cacatagagc gccttgccct
    10321 tcgcgtcgta cgcgaccgac tcgggaaaga cgaagccgcc gaccgcctgg tcggacgaga
    10381 cgcgccattg cgcaagcgcg ggttgagaaa taagacctaa gagaacgatc gcgaagcgga
    10441 gcttcatggg ccacctccct gaggatgcgc gcactctatc gtgaaaaatg tacagggggc
    10501 ttgacgtccg gcggtgccgt gcttacaacg ataggaggtt cagcaggctt ggggaggcct
    10561 tatgcggacc gtcgccctca cgatcgctct tgccggcgcc ttgcacgccg gagtttcgct
    10621 ggcgcaacgg cgcccggcaa cggaagccga agcgatgtcc tacatcacct cggcgttcat
    10681 ctcgcaggcc gaccctgctg ccatgagtcc cggcgtcgcg ctcggccccg agctcgagaa
    10741 ggcgctcggc ctgcagcccg gcaccgaccg ggtgaaggtc taccaggcgg tggtcccgct
    10801 cgccgacgat ccgcggctcg aggtgcggcg cgccgcgccg gccgatgtcg ccgactacgg
    10861 cgcgcgccgc ggcttcgacc ccgcgtcgcc gcatcccctt ttcaccgtgc agacggcaaa
    10921 ggtgaagctc ctggtccagt atgacctgaa ggcgttcaac atcgtgttcg tcggccagct
    10981 cggcatgcct gatcctgatc cggtcgtggt ggcgaaggcc gacccggcgg cgccggcgcc
    11041 cagccagccc gcgctcgtca atttcgcctg gacggcggag ttctcctacg gcagcgccga
    11101 gctcactccc gaagcgcgcg ccaggctcga cggcgagatc gtgccgaagc tcatcgcgtc
    11161 gaaggaaatc cgctacctca acgtctacgg gcacagcgac cgcacgggct ccgccgagta
    11221 caaccgccgg ctgtcggaga agcgcgccga ggcggtgcgc gactacctcg tctcgaaggg
    11281 cgtcgacccg gacaagattg aggtgttcgg ctatggccag acgctgccgg tgaaatcctg
    11341 ccgcgacgag aaggagcgcg cgctgatcga gtgcctcgcg ccgaaccgcc gcatcgtggt
    11401 ggagatccag gctacgccgt agccctgcag ttatggcgtg gcccatcggc gggagcggcc
    11461 caccgcctcg cgccagcgcg tcatcctggc ctcactgtcg gaggcgctca tccgcggctc
    11521 gaagcggcgc tcggctttcc agtgcgacgc gagctcgtcg cgcgacttcc acaggtcgac
    11581 cgtgaggccg gcaaggtagg ctgcgccgag cgcggtggtc tcgagcactt tcgggcgcac
    11641 caccggaacg ccgagcaggt cggcctggaa ctgcatcagc aggtcgttcg ccgcggcgcc
    11701 gccatcgacg cgcagctcgc tcaatttttc cccggcatcc ttttgcatgg cctcgagcaa
    11761 ttccgcgctc tggaatgcaa tcgactcgag cgcggcccgg gcgatgtgcg ctttggtggt
    11821 tcctctcgtc aacccgacga tcgtgcctcg cgcgtccggg tcccagtagg gtgcaccgag
    11881 accggtgaat gcggggacga catagacgtc accgctatcc aagacgctgg cggccagctt
    11941 ttcgatttca gcagaagagg aaaagaactc gaggccgtct cgcaaccact gcaccaccgc
    12001 gccgccgatg aacacgctgc cttccagcgc gaattccttg ccgcgggtct gcgcgcacgc
    12061 cgtcgagatg agcccgtgag acgactgcac gaccttgtcg ccggtgtgca gcagcatgaa
    12121 gcagccggtg ccgtaggtgt tcttcgccat gccggcgcgg tggcaggcct ggccgaacag
    12181 cgccgcctgc tggtcgccgg cgacgccgcc gatgaccagc ggctcgccga ggacgcccgc
    12241 cggcagcatg ccgaacgcgt gcgccgacgg atgcacgtcc ggcaggatcg cgcgcggcac
    12301 gtcgaggagc cgcagcagtt ccggatccca gtcgttcatg tggatgttga agagcattgt
    12361 gcgcgaggcg ttggagacgt cggtgacgtg ggtgcggttg ccggtgagat gccacgtgag
    12421 ccaggtgtcg accgtgccga acgcgagctc accgagctcg gcgcgcttgc gcgcgcccgg
    12481 aacgttgtcg aggagccacg cgatcttggt gccgctgaag tacggatcga tcacgagacc
    12541 cgtgcgctcg cgcaccaggc cctccgcgcc gatctgcttc agctccgcgc actgcggcgc
    12601 ggtgcggcga tcctgccaga caatcgcatt cgcgatcggc tcgccggtct ggcggtccca
    12661 cacgatggtg gtctcgcgct ggttggtgat gccgaccgcc atcaggtttt tcagaggaat
    12721 accggattgg cttactgcct gccgtgccgt ttccctttgg gtggagaaaa tctccttcgg
    12781 gtcgtgctcg acccagccgg gctgcggaaa gatctggcgg aattcgcgct gcgcggtcga
    12841 aaccgggcgg ccttcctcgt cgaacaggat cgcgcgagag ctggtcgtgc cttggtcgag
    12901 agcgaggatg gtgcgcaccg ggtgattcta ctttttccga taaaatccgc gccttatgcc
    12961 gctgccggtc accgacgtcg agcgcgagct gcgccacacg cgccgcgtgc gcttcgaagg
    13021 ctacaagcgc gccgacgggc tgtgggacct cgaggcgcac ctcaccgaca tcaagagcca
    13081 cgacaacccg atgaagaccg gcgtgcgccg cgccgggcag ccgatccacg agatgtggat
    13141 ccgcctcacc atcgaccggc acctgaccat cctcgatgcg tccgcgagct ccgacgcggt
    13201 gccttatccc ggcggctgcg agaccatcgc gcccgcctac aagcgcctga tcggcttgaa
    13261 tctcgttcgc gacttccgca gggcggtgaa gaagcatttc ggcgacgtga aggggtgcac
    13321 ccacctcacc gagatgctgg ccggctttcc caccgcggcg atccagacct tcgccggcga
    13381 gatgcccgaa gagcgcaccg acggccgcaa gccattccag ctcgaccagt gccacgcgct
    13441 cgacaccgcc ggcgaaacgg tgaagaagtg gtatccgaag tggtatagcg gaaaaagcgg
    13501 caaggagaaa gcgccggcat agatgaaaat ccacgagtac caaggcaaag aaatcttccg
    13561 caagtacggc ctccccacgc cgcgcggctt ccccgccttc agcgtggacg aagcggtcga
    13621 ggcggcgaag aagctcggcg gcaaggtctg ggtggtgaaa gcgcagatcc acgccggcgg
    13681 ccgcggcaag ggcggcggcg tcaaggtggc gaagtcgctg gaggaggttc gcaagctttc
    13741 ctcttccatc ctggggatgc aattgaaaac ccatcagacc ggccccggcg gccagaaggt
    13801 gcggcgcctg ctggtcgagg agggcgccga catcaagaag gagctctacg tcggcatggt
    13861 ggtggaccgc ggcacgcaga aggtcgcgct gatggcgtcc tccgaaggcg gcatggacat
    13921 cgaggaagtc gcggcgaaga cgcccgagaa aatccacaag gtgttcatcg acccggcgca
    13981 gggcctctcc gacgccgacg ccgacgagat cgcgctcgcc atcggcattc cgcagtccgc
    14041 gctggggcag gggcgcgcga tcttcaaggc gctctacaag tgcttcgacg aaacggacgc
    14101 ctcactggcg gagataaacc ccttaatagt gacaaattca aatcaggtcg tcgccctcga
    14161 ttccaagctc aacttcgacg acaacgcgct tttccgccat cccgaaatcg cggcgctccg
    14221 ggacctggac gaggaggacc cgttcgagat cgaggcgtcg aagtacgagc tgaactacat
    14281 ctcgctcgac ggcaacatcg cctgcatggt gaacggcgcc ggcctcgcca tggcgacgat
    14341 ggataccatc aagctgttcg gcggctcgcc ggccaacttc ctcgacgtcg gcggcagcgc
    14401 caccgccgag cgggtgacgg cggcgttcaa gatcatgctg tcgaacaaga aggtgaaggc
    14461 catcctggtg aacattttcg gcggcatcat gaaatgcgac accatcgccg ccggcgtcgt
    14521 cgcggctgcg cgcgaagtga agctctcggt gccgctggtg gtgcgcatga aaggcaccaa
    14581 cgaggatctc gggaagaaaa tcctcaagga ctccggcctg ccgatcatct cggcggacaa
    14641 catggccgat gcggcgaaga aggtggttgc cgcggtgaag ggcaagaaga ccaagcctgc
    14701 gaagaagaaa gtcatgctca ggaaaacgaa aaaggctgcc gcaaagagga agtcaaagaa
    14761 gaagggaaag aagaaatgag cgccgtcttg ctttgccccc ctgacaaggg gggtggcgag
    14821 ggagtttctc gcgagccggg gggttgcctt tcgaaaaacc aaaccccccg ttcgccttcg
    14881 gctcacgagc ttttccccct cccagggggt gtcagggggt cgaaccagaa cctatgagca
    14941 tcctgatcga caagaacacg cgcgtgatga cccagggcat caccggcaag accgggatgt
    15001 tccacaccca gacgagccgc gcctacgcca acggaaaagc gtgcttcgtc gccggcgtca
    15061 atccgaagaa ggcgggcgag aacttcgagg gcattccgat ctttggttct gttcttgaag
    15121 ccagaaaaac tacaggggca accgtatcgg tgatctacgt gccgccgccc ttcgccgcgg
    15181 cggcgatcga ggaggcggtc gacgcggacc tcgatctcgt gatctgcatc accgagggaa
    15241 taccggtgcg cgacatgatc cgcaccaagg accgcatgaa gggcaggcgc acgctgctgg
    15301 tcgggccgaa ctgcccgggc gtgatcacgc ccgacgagat caagatcggc atcatgcccg
    15361 gccacatcca caagaagggt ccgatcgggg tcgtgtcgcg ctcgggaacg ctgacctacg
    15421 aggcggtcgg gcagctcagc gagctcggcc tgggccagtc gagctgcgtc ggcatcggcg
    15481 gggatcctgt caacggggtg aagcacatag acgttcttcg gatgttcaac gaggacccca
    15541 agaccgaggc cgtcatcatg gtcggcgaga tcggcggctc ggacgaggaa aacgcggcgc
    15601 aatgggtgaa gaagaacatg aaaaagccgg tggtggggtt catcgccggg atcaccgcgc
    15661 cgccgggcaa gcgcatgggc catgccgggg cgatcatctc cggtggcaag ggcacggcgc
    15721 aggagaagct ggcggtgatg gaagcgtgcg gcatcaaggt gacgcgcaac cccgcggaga
    15781 tgggaaagac gctgaagtcc gtcctgagat agagcgattc ataccctagc aacccggcac
    15841 tcggtggtcg ccacgctgcc ctacgcggcg acgatctttc tttcgtcgtt cctgctgttc
    15901 ctggtgcagc cgatcatcgc caagcagatc ctgccgtggt tcggcggctc ggcgggcgtg
    15961 tggacgacgt gcctggtctt cttccagagc gtgctgctcg cgggctacgc ctatgcggac
    16021 tggacgacgc ggctcggcgc ccgccgccag gcaatgttgc acgtcgcgct gctcgcggcc
    16081 tcgcttctct gcctgccgat cctcgcctcg actggctgga agccgcaggg cgacgagcag
    16141 ccgatcctgc gcatcctgct cctgctcggc gcgacgatcg ggctgccgta cttcctgctc
    16201 tcgaccacca cgccgctcct ccaggcgtgg tattggcgcc gcttccgctc ggtggtgccc
    16261 tatcgcctgt tcgcgctttc gaacctcgcg tcgcttctcg cgctgctcgg ctttccggtg
    16321 ctgttcgagc cggcgttcga cctgaccagc ctgggctggg gatggtcgtt cctgttcgcc
    16381 gggttcgcga ttctctgcgc
//
LOCUS       DATBSZ010000044         8945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1371585, whole genome shotgun sequence.
ACCESSION   DATBSZ010000044 DATBSZ010000000
VERSION     DATBSZ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8945
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371585"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VIG70_02965"
     CDS             complement(<1..511)
                     /locus_tag="VIG70_02965"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HEY8249539.1"
                     /translation="MNTKATPRTTHRTSAWLVGLAGLALFATQAWAQNTIDAFEVSQQ
                     GGKTVVRVTTKEPLKSVPPNFSVATPARIAFDFPNTANALGRSSQDIGQGDLRSMNVV
                     QGAERTRLVLNLRRAVGHEAALEGNTLVITLAEPAIAATPPAGVAHFAEGKADVKHAV
                     RDIDFRRGRA"
     gene            complement(508..1044)
                     /locus_tag="VIG70_02970"
     CDS             complement(508..1044)
                     /locus_tag="VIG70_02970"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEY8249540.1"
                     /translation="MSAGVRLVMLAAALALAACGGDEHSDLKEELHQLTKDLRGRVEP
                     LPQVKPYEPVPYTAEGQVDPFRTERIEVASAGRATGASVALIEEQKKRPPEPLEAFPL
                     ESIQMLGTITQNKETFALVKAGPNLYRVKKGNYMGQNFGVITAIDEAQISLKELVQDS
                     TGDWVERISSLQLVEAKR"
     gene            complement(1041..1688)
                     /locus_tag="VIG70_02975"
     CDS             complement(1041..1688)
                     /locus_tag="VIG70_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEY8249541.1"
                     /translation="MALNFNAIADEFKRTNWKDAGTWGAAPKVVVLLAVLVGVPVAGF
                     FVFNQGQIEELEAGQQKEAKLKEEYVGKKKQAINLDLHRQQLREIDTQFGALLKQLPN
                     KSQMDALLVDINQAGLGRGLQFELFKPAAAEMRREFYAELPIDVKVTGTYHDMGAFAS
                     DVGQLSRIVTLKDVNIVAGRDGNLTMDAIARTFRYLDDDEIAAQRKAATQKGAKK"
     gene            complement(1693..2292)
                     /locus_tag="VIG70_02980"
     CDS             complement(1693..2292)
                     /locus_tag="VIG70_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEY8249542.1"
                     /translation="MNARINLLPHRAERRKRARQHFMTVSGMTVGLGILVAFLIYQFY
                     ARQIEVQTDRNTFLANEIKKLDKDIADINELRNQIQALLARKQIIETLQADRAQTVHL
                     LEQMVRQMPEGVYLKSMKQAGAKVHVVGYAQSNARVSTLMRNIEASPWLTQPVLVEVK
                     AANVDKRRVSEFNMYLNLKRAEPPKEGAKSAPPAAAKKG"
     gene            complement(2289..3365)
                     /locus_tag="VIG70_02985"
     CDS             complement(2289..3365)
                     /locus_tag="VIG70_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY8249543.1"
                     /translation="MAFDLFTAKAPPLFGLDISSSSVKMLELVDGGKSGYRVERYAIE
                     PLPRDSVVDGNINNLEAVTESVKRAHGRMGTRTKHVAMAVPSGAVISKKIIVPATLRE
                     EELEVQVESEANQYIPFALEEVNLDFQILGPSATNPEEQEVLIAATRKEKVEDRIAVA
                     DSAGLKALVMDVESFAQQTALSLVVQGLPGGGRDQNIAVVDVGANVMQVTVLRNDQSV
                     YTREQAFGGNQLTQDIVSRYGMTPEEAENAKRSGGLPDDFEAEVMKPFMENLSMEVQR
                     ALQFFFTSTQYHAVDHILLAGGSAVIPGLDEVVHTRAQVPTSVANPFSLMQASPKVQL
                     KRLMVDAPSLIVACGLAMRRFDPV"
     gene            3557..5872
                     /locus_tag="VIG70_02990"
     CDS             3557..5872
                     /locus_tag="VIG70_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEY8249544.1"
                     /translation="MWIRFVAFPLAVLAGLAVAAILGVGLVVVLAYPQLPSLEALTAY
                     QPKIPLRIYSAEGVLIGEFGEERRSFISIAEVPQQLKNAVIAAEDERFYEHPGIDYVG
                     VLRAAWANLVAGGRRQGASTITMQVARNFFLSSEKTLTRKLYEALLAFKIEHSLSKEQ
                     ILELYVNQIFLGQRSYGFAAASQAYYGKALGQLSLAEISMLAGLPKAPSMNPVSNPQR
                     AKQRQQYVLRRMTELGYIDATQYEDALKAPLRTRREVTEYSVHAEYAAEMVRQALAEH
                     YPEDVYTRGFRVYTTIRKADQEAAYVALRAGVMEYDRRAGYRGPEGYVELPATPDDDD
                     LEDALADHPDNDDLLAAVVLAYDGKQVQAGLRSGERLAVQGQGLQFAARALDAKAAPQ
                     RRIRKGAIIRVQREGKNWQIVQLPEVEAAFIALDPQDGAIRALVGGFDFSRNKFNHVT
                     QAWRQPGSSFKPFIYSAALEKGFTAATVIPDEPVVLEAEQTGSQRWEPKNYDGKFEGP
                     MRLRTALAKSKNMVSIRILDAIGPRYAQEYVTRFGFDPERHPPYLTMALGAGSVTAWQ
                     LARAYSVFANGGFLIQPYFIHKIVDDRGNALALADPRRAGDEGLRVLDPRNAFIMDNM
                     MQDVTRSGTAARAARLGRTDLAGKTGTTNEFVDAWFAGYQPALVGVSWVGFDQPKTLG
                     KNQTGGVVALPIWLGYMERVLGDIPEMSRTVPDGVVAVPTMQYPAPAGEAKTIPEFFY
                     REAVPPPEVLQPPVPVQPPPVLPEPQPNAPA"
     gene            complement(5840..6157)
                     /gene="cyaY"
                     /locus_tag="VIG70_02995"
     CDS             complement(5840..6157)
                     /gene="cyaY"
                     /locus_tag="VIG70_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEY8249545.1"
                     /translation="MDEAEFAAQASVVLDALERAFEEIDLDFELKEGGILEIEFEDGG
                     KIIVNRHAAAREIWVAARSGGFHYRWDGTAWRDTRSGAELFGALSDLLSSQLGRRVRL
                     RLG"
     gene            6177..6341
                     /locus_tag="VIG70_03000"
     CDS             6177..6341
                     /locus_tag="VIG70_03000"
                     /inference="COORDINATES: protein motif:HMM:NF025012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEY8249546.1"
                     /translation="MRFLVLVAACALAAGCGQKGPLYLRDSPPPGIKPAKPKAYEPVP
                     YPRELTEEEK"
     gene            6347..7588
                     /gene="lysA"
                     /locus_tag="VIG70_03005"
     CDS             6347..7588
                     /gene="lysA"
                     /locus_tag="VIG70_03005"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY8249547.1"
                     /translation="MPFTYRKGVLCADEVALDDIARRFGTPCYVYSAAAIEAAYAEFA
                     QSLKGRDARVCYSVKANSNLAVLALLVSLGSGFDIVSGGELARVIAAGGDPRKTLFSG
                     VGKSEAELRFALERGIGCINLESEAELTRVNAVAEKLGKRAPVAFRVNPDVDARTHPY
                     ISTGLRETKFGVAHADAERLYRKAQAMKGVELVGIGCHIGSMLQESAPYVAAVERLVA
                     LVDRLERGGIRLTHIDVGGGIGIRYRDEAPQPPAAFVAGTLAALGERPHTVVLDPGRS
                     IVGNAGVLLTRVEYVKPGAAKSFLVVDAAMNDLIRPALYGAWHDVKTVAESVPGNAVY
                     DVVGPVCESADFLAKDRSLAAAEGDLLAIMSCGAYAMVMSSNYNTRPRAAEVLVSRGE
                     AHLVRRRESVEDLFALEHIQG"
     gene            7602..8780
                     /locus_tag="VIG70_03010"
     CDS             7602..8780
                     /locus_tag="VIG70_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8249548.1"
                     /translation="MTTATRRLALFGLVVVVLAAGVGAYFFAGDGRAARKESAKGAAK
                     GGPQAVLVTSAVVQARTLELYEEVLGTIENVIDPTIGAEVAGRVTRVAGFTGKKVAKG
                     ELIAEIDAADFEIQARGDRAEIGRLTGLLEQQERVVERQTKLVGQGFISQNVVDDTVA
                     QRNALRQQLAAARARADATGRGLTKSRVLAPVDGEIETQIVAIGDYVKVGDPLFKLVG
                     VQQLRVHLLLPEAAAVRIKPGLKVVLSPPAAPEQILEARIDEIKPTLGINNRAIDAIV
                     KINGVGTLFRGGGTVNARIVTNSLDGALMVPEQSVVLRPAGKVVYALEGNVARQRIVE
                     TGMRQDGWQQVVKGLSAGETVAADGAGFLTDGAAVTVRAPRGAEKGTAEESAKGARKG
                     "
BASE COUNT         1394 a         3034 c         2988 g         1529 t
ORIGIN      
        1 cggcgcggcc gcggcggaaa tcgatgtcgc gcaccgcgtg cttgacgtcg gccttgccct
       61 cggcgaaatg cgccactccg gccggcggcg tggcggcgat cgccggctcg gcgagcgtga
      121 tcaccagcgt gttgccttcg agcgcggctt cgtggcccac cgcgcggcgc aggttgagca
      181 ccaggcgcgt gcgctcggcg ccctgcacca cgttcatgct gcgcaggtcg ccctggccga
      241 tgtcctggct ggagcggccg agcgcgttgg cggtgttcgg aaaatcgaag gcgatgcgcg
      301 cgggggtcgc gaccgagaag ttcggcggca cgctcttcag cggctccttg gtggtgacgc
      361 gcaccaccgt cttgcccccc tgctggctga cctcgaaggc gtcgatggtg ttttgcgccc
      421 aggcctgcgt ggcgaagagc gcaaggcccg ccaggccgac gagccaggcc gaagtgcggt
      481 gagtggtgcg gggagtggct ttggtattca tcgttttgcc tccaccagtt gcagcgagct
      541 gatgcgctcc acccagtcgc cggtcgagtc ctggaccagc tccttcaagc tgatctgcgc
      601 ctcgtcgatc gccgtgatga cgccgaaatt ctggcccatg taattgccct tcttcacccg
      661 gtacaggttg gggccggcct tcaccagggc gaaggtttcc ttgttctggg tgatggtgcc
      721 gagcatctgg atcgactcga gcgggaaggc ttcgagcggc tcgggcgggc gcttcttctg
      781 ctcctcgatg agcgcgaccg aggcgcccgt ggcgcggccg gccgacgcca cttcgatgcg
      841 ctcggtgcgg aacgggtcga cctggccctc ggcggtatac ggcaccggct cgtagggctt
      901 gacctgcggc agcggctcga cgcgcccgcg caggtctttg gtgagctggt gcagctcctc
      961 cttgaggtcg ctgtgctcgt cgccgccgca ggcggcgagc gcgagcgccg cggcgagcat
     1021 caccaggcgt acgcccgcgc tcacttcttc gcgcctttct gggtcgccgc cttgcgctgc
     1081 gccgcgatct cgtcgtcgtc gaggtagcgg aaggtgcgcg cgatcgcgtc catcgtcagg
     1141 ttgccgtccc tgccggcgac gatgttcacg tccttgagcg tgacgatgcg ggagagctgg
     1201 ccgacgtcgc tcgcgaacgc gcccatgtca tggtaggtgc cggtcacctt cacgtcgatc
     1261 ggcagctcgg cgtagaactc gcgccgcatt tcggccgcgg cgggcttgaa cagctcgaac
     1321 tgcaggccgc ggccgaggcc ggcctggttg atgtcgacca gcagcgcgtc catctgcgac
     1381 ttgttgggca gctgcttcag cagcgcgccg aactgggtgt cgatctcgcg cagctgctgg
     1441 cggtgcaggt cgaggttgat cgcctgcttc ttcttgccga cgtactcttc cttgagcttc
     1501 gcctccttct gctggccggc ctcgagctcc tcgatctgcc cctggttgaa gacgaagaat
     1561 ccggcaaccg gcacgccgac caggactgcg agcagcacca cgaccttggg cgctgcgccc
     1621 caagtgcctg cgtccttcca gttggttcgc ttgaactcgt cggcgatcgc gttgaagttg
     1681 agcgccatgg ccttatccct tcttcgccgc cgccggcggc gccgacttcg cgccctcctt
     1741 cggcggctcg gcgcgcttca ggttcaggta catgttgaat tcggagacgc gccgcttgtc
     1801 gacgttggcc gccttgacct cgaccagcac cggctgcgtc agccacggcg aggcttcgat
     1861 gttgcgcatc agcgtcgaca cgcgcgcgtt cgactgcgcg tagcccacga cgtgcacctt
     1921 cgccccggcc tgcttcatcg atttcaggta cacgccctcg ggcatctggc gcaccatctg
     1981 ctcgagcagg tgcacggtct gggcgcggtc ggcctgcagc gtctcgatga tctgcttgcg
     2041 cgcgagcagc gcctggatct ggttgcgcag ctcgttgatg tcggcgatgt ccttgtcgag
     2101 cttcttgatc tcgttggcga ggaaggtgtt gcggtcggtc tgcacctcga tctggcgggc
     2161 gtagaactga tagatcagga aggcgaccag gatgccgagg ccgacggtca tcccggagac
     2221 cgtcatgaaa tgctgccgcg cgcgcttgcg ccgctcggcg cggtgcggca gcaggttgat
     2281 gcgcgcgttc ataccgggtc gaacctcctc atcgccaggc cgcaggcgac gatcagagaa
     2341 ggggcgtcca ccatcagccg cttgagctgg accttcggcg acgcctgcat cagcgagaac
     2401 gggttggcga cgctcgtcgg cacctgggcg cgcgtgtgca ccacctcgtc gagcccgggg
     2461 atcaccgccg agccgccggc gagcaggata tggtcgaccg cgtggtactg ggtcgaggta
     2521 aagaagaact gcagcgcgcg ctgcacttcc atcgacaggt tttccatgaa aggcttcatg
     2581 acctcggctt cgaagtcgtc gggcaggccg cccgagcgct tggcgttctc cgcctcctcc
     2641 ggggtcatac cgtagcggct gacgatgtcc tgggtgagct ggttgccgcc gaacgcctgc
     2701 tcgcgcgtat atacggactg gtcgttgcgc agcaccgtca cctgcatcac gttggcgccg
     2761 acgtcgacca cggcgatgtt ctggtccctg cccccgccgg gcaggccttg caccacgagc
     2821 gaaagcgcag tctgctgggc aaaggactcg acgtccatca ccagcgcctt gaggccggcg
     2881 gaatcggcga ccgcaatccg gtcctcgacc ttttcctttc gcgtcgccgc gatcaggact
     2941 tcctgctcct cgggattggt cgccgacggg ccgaggatct ggaaatccag gttgacttcc
     3001 tcgagcgcga acgggatgta ctggttggcc tcgctctcga cctgcacctc gagctcttcc
     3061 tcgcgcaagg tggccgggac aatgatcttc ttgctgatga cggcgccgct cggcaccgcc
     3121 atcgcgacgt gcttggtgcg ggtgcccatg cggccgtggg cgcgcttgac cgactcggtc
     3181 accgcctcga ggttgttgat gttgccgtcg accacggagt cgcgcggcag cggctcgatg
     3241 gcgtagcgct cgacgcgata gccgctcttg ccgccatcga cgagctcgag catcttcacc
     3301 gatgaagagc tgatgtccag cccgaacagc ggtggcgcct tcgctgtgaa gaggtcgaat
     3361 gccatttggt ccttccccgg tatttccctt ttcgcgtcgg ctcttaggcg cgattcgcag
     3421 aaaccgcctt aaatgctagc aacaagtccc acggctgtct acttaacttt ccacaggggg
     3481 taagggggta ttatcccggc ggtccggtca ccccttgcaa caacgcccgt cccgacccgt
     3541 tctgagccac aagaacatgt ggatccggtt tgtcgccttc cccctcgccg tcctggccgg
     3601 attggctgta gccgccatct tgggtgtggg cctggtggtg gtccttgcct atccccagct
     3661 gccctcgctt gaggccctca ccgcctacca gcccaagatc cccctgcgca tctacagcgc
     3721 cgaaggggtt ctgatcggcg agttcggcga ggagcgccgt tcgttcattt cgatcgccga
     3781 ggtgccgcag cagctcaaga acgcggtgat cgccgccgag gacgagcgct tctacgagca
     3841 tcccggcatc gactacgtcg gcgtgctgcg cgccgcctgg gcgaacctgg tcgccggcgg
     3901 ccggcgccag ggggcgagca ccatcacgat gcaggtcgcg cgcaacttct tcctgtcgtc
     3961 ggagaagacc ctgacgcgca agctgtacga ggcgctgctg gccttcaaga tcgagcacag
     4021 cctgtcgaag gagcagatcc tcgagctcta cgtcaatcag attttcctcg ggcaacgctc
     4081 ctacggcttt gcagcggcct cccaggctta ttacggcaag gccctcggcc agctctcgct
     4141 cgccgagatc tcgatgctcg ccgggctgcc caaggcgccg tcgatgaacc cggtttccaa
     4201 tccgcagcgc gccaagcagc gccaacagta cgtgctgcgc cgcatgaccg agctcggcta
     4261 tatcgatgcc acgcagtatg aggacgccct gaaggcgccg ctgcgcaccc ggcgggaggt
     4321 caccgagtac tcggtgcatg ccgagtacgc cgccgagatg gtgcgccagg cgcttgccga
     4381 gcactacccg gaggatgtgt atacccgggg gttccgcgtc tacaccacga tccgcaaggc
     4441 cgatcaggaa gccgcgtacg tcgccctgcg cgccggggtg atggagtacg accgtcgcgc
     4501 cggctatcgc ggcccggaag gctacgtcga gcttccggcc acccccgacg acgacgacct
     4561 cgaggacgcg ctcgccgatc acccggacaa cgacgacttg ctcgccgcgg tggtcctcgc
     4621 ctacgacggg aagcaggtgc aggccgggct gcgcagcggc gagcgcctcg cggtgcaggg
     4681 ccaggggctg caattcgccg cccgtgcgct cgacgcgaaa gccgccccgc aacggcgcat
     4741 ccgcaagggc gccatcatcc gcgtgcagcg cgaaggcaag aactggcaga tcgtgcagct
     4801 acccgaggtc gaagccgcct tcatcgcgct cgacccgcag gacggcgcga tccgggcgct
     4861 cgtcggcggc ttcgacttca gccgcaacaa gttcaaccac gtgacccagg cctggcgcca
     4921 gccgggatcg tcgttcaagc cgttcatcta ttcggccgcc ctcgagaagg gattcaccgc
     4981 cgcgaccgtg atcccggacg agccggtggt gctcgaggcg gagcagaccg gcagccagcg
     5041 ctgggagccc aagaactacg acggcaagtt cgagggcccg atgcgcctga ggaccgcgct
     5101 cgcgaagtcg aagaacatgg tgtcgatccg catcctggat gcgatcgggc cccggtacgc
     5161 ccaggagtac gtcacgcgct tcggcttcga ccccgagcgg cacccgcctt acctgaccat
     5221 ggcgctcggc gcgggatcgg tgaccgcctg gcaactggcg cgcgcctact cggtgttcgc
     5281 gaacggcggc ttcctgatcc agccgtactt catccacaag atcgtggacg accgcggcaa
     5341 cgcgctggcc cttgccgacc cccggcgcgc gggcgacgag ggcctgcgcg tgctcgatcc
     5401 gcgcaacgcc ttcatcatgg acaacatgat gcaggacgtc acgcgctccg gcaccgccgc
     5461 gcgcgccgcg cggctcggcc gcaccgacct cgccggcaag accggcacca cgaacgagtt
     5521 cgtcgatgcc tggttcgccg gctatcagcc ggcgctggtc ggggtgtcgt gggtcggctt
     5581 cgaccagccc aagacgctcg gaaagaacca aaccggcggc gtggtcgcgc tgccgatctg
     5641 gctcggctac atggaaagag tgctcggcga tatccccgag atgtcgcgca ccgtccccga
     5701 cggcgtggtc gcggtgccga caatgcagta tccggcgcct gccggcgagg cgaagacgat
     5761 tccggaattc ttctaccgcg aggcggtccc gccacccgaa gtcctgcagc cgccggtacc
     5821 ggtgcagccg ccgccggtgc tacccgagcc gcagccgaac gcgccggcct agctggctgg
     5881 agagaaggtc cgaaagggcg ccgaagagtt cggcgccgct ccgcgtgtcg cgccaggccg
     5941 tcccgtccca ccggtaatga aacccgccgg agcgtgccgc cacccagatc tcgcgcgccg
     6001 ccgcgtgccg gttgacgatg atcttgccgc cgtcttcgaa ctcgatctcg aggatgcccc
     6061 cctccttaag ctcgaagtcc aaatcaatct cctcgaacgc ccgctccaga gcgtcgagca
     6121 cgacagacgc ctgcgctgcg aattcggctt cgtccatggg gtaacatgaa gattctatgc
     6181 gcttccttgt gctcgtcgcc gcttgcgcgc tggccgccgg ctgcgggcag aaggggccgc
     6241 tctacctgcg cgacagcccg ccgccgggta tcaagcccgc caagcccaag gcttacgagc
     6301 cggttcccta tccgcgcgag ctgaccgaag aagagaagta ggcgccgtgc ccttcaccta
     6361 ccgcaagggc gtgctgtgcg ccgacgaggt cgccttggac gacatcgcgc gccgcttcgg
     6421 cacgccctgc tacgtgtact ccgcggcggc gatcgaagcc gcctacgctg aatttgccca
     6481 gtccctgaaa gggcgcgacg cgcgggtctg ctactcggtc aaggccaatt cgaacctcgc
     6541 ggtgctggcg cttctcgtca gtctgggcag cggcttcgac atcgtgtcgg gcggcgagct
     6601 tgcgcgcgtc atcgccgccg gcggcgatcc gcgcaagacg ctcttctccg gcgtgggcaa
     6661 gagcgaggcc gagcttcgct ttgcgctcga gaggggcatc ggctgcatca atctcgagtc
     6721 cgaagcggaa ttgacaagag ttaacgccgt tgccgagaag ctcggaaagc gcgcgccggt
     6781 cgcgttccgc gtcaacccgg acgtcgacgc caggacccat ccctacatct cgactggcct
     6841 gcgcgagacc aagttcggcg tggcgcacgc cgatgccgag cgcctttacc ggaaagcgca
     6901 agccatgaag ggcgtggagc tcgtcgggat tggctgccac atcggctcga tgctgcagga
     6961 gagcgcgccc tacgtcgccg ctgtcgagcg gctggtcgcg ctcgtcgacc ggctggagcg
     7021 cggcggcatc cgcctgacgc acatcgacgt cggcggcggg atcggcatcc gctaccggga
     7081 cgaggctcca cagccgccgg cagcgttcgt tgccggcacc ctggccgcgc tcggggagcg
     7141 cccccacacg gtcgtcctcg acccgggccg ttcgatcgtc ggtaacgcgg gcgtgctact
     7201 aacgcgcgtc gaatacgtga aaccgggggc agcgaagagc ttcctcgtgg tcgatgcggc
     7261 catgaacgac ctcatccggc ccgcccttta cggagcgtgg catgacgtga agaccgtggc
     7321 ggaatcggtt ccgggcaacg cggtctacga cgtcgttggc ccggtgtgcg agagcgcgga
     7381 tttcctcgcc aaggaccgca gcttggccgc agccgaggga gacttgctcg cgatcatgtc
     7441 ctgcggcgcc tatgccatgg taatgagttc gaactacaac acccggcccc gcgctgcgga
     7501 ggttctggtg agcagggggg aagcccattt ggtgcgccgc cgcgaatcgg tggaggacct
     7561 gtttgcccta gagcacattc agggctgaca atcgccggtc catgaccact gccacccgcc
     7621 gcctcgcgct ttttggcctc gtcgtcgtcg tcctggcggc cggtgtgggc gcgtacttct
     7681 ttgccggcga cggccgcgcg gcgaggaagg agagcgccaa gggggccgcc aagggaggcc
     7741 cgcaggcggt cctcgttacc tcggcggtgg tgcaggcacg cacgctcgag ctttacgaag
     7801 aggtgctggg gacgatcgag aacgtcatcg atccgaccat cggcgccgag gtggccgggc
     7861 gcgttacgcg cgttgccggc ttcacgggca agaaggtcgc caaaggcgag ctcatcgccg
     7921 agatcgatgc cgcggatttt gagatccagg cacgcggcga cagggccgag atcggccgcc
     7981 taacgggcct gctcgagcag caggaaaggg tggtcgagcg ccagaccaag ctcgtcggcc
     8041 agggattcat ctcgcagaac gtggtcgacg acacggtcgc gcagaggaac gccctgcgcc
     8101 agcagctcgc cgccgcgcgc gcgcgcgccg acgccaccgg ccgcggcctc accaagtcgc
     8161 gcgtgctcgc cccggtcgac ggcgagatcg agacccagat cgtcgccatc ggcgattacg
     8221 tcaaggtggg cgatccgctg ttcaagctgg tcggggtgca gcagctgcgg gtccatttgc
     8281 tgctgcccga ggctgccgcg gtgcgcatca agccgggcct gaaggtggtg ctgtccccgc
     8341 ccgcggcgcc cgagcagatt ctggaagccc gcatcgacga gatcaagccg acgctcggca
     8401 tcaacaaccg cgcgatcgac gcgatcgtca agatcaacgg cgtgggcacg ctgttccgcg
     8461 gcggcggcac ggtcaacgcg cgcatcgtga cgaactcgct tgacggcgcg ctgatggtcc
     8521 ccgagcagag cgtggtgctg cgccccgccg gcaaggtggt ctacgcgctc gaaggcaacg
     8581 tcgcgcgcca gcgcatcgtc gagaccggca tgcgccagga cggatggcag caggtcgtga
     8641 agggcctgag cgccggcgag acggtcgcgg ccgacggcgc cggcttcctc accgatggcg
     8701 ccgccgtgac ggttcgggcg ccccgcggcg cggaaaaggg caccgcagaa gaaagcgcga
     8761 aaggcgcgcg caagggctag gcgatgaccc tctccgagct ctcgatccgg cgctacgtct
     8821 tcgccttcat gttgagcgcc ctgctggtgc tgttcggcgc gatcgcgtac cagcgcatgg
     8881 gcgtcgacaa gctgccgtac atcgagtttc cgatcatctc catcaccacg gcgtacaagg
     8941 gcgcg
//
LOCUS       DATBSZ010000045         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_347578, whole genome shotgun sequence.
ACCESSION   DATBSZ010000045 DATBSZ010000000
VERSION     DATBSZ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347578"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VIG70_03015"
     CDS             <1..250
                     /locus_tag="VIG70_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249549.1"
                     /translation="AAAAALLVPVGVLLGVGLPGGMRLLNRARPEIVAWGWGMNGAFS
                     VIGATAAIFIAMNWGFSVTLLAGALTYLLAAGCLKMCR"
     gene            247..1188
                     /locus_tag="VIG70_03020"
     CDS             247..1188
                     /locus_tag="VIG70_03020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8249550.1"
                     /translation="MKLVSLVVPFHNEAGGVEAFHREVSTALNALPAYRFEIVCVDDG
                     SSDATLQGLLQLVERDRRVRVLELSRNFGKEAALTAGLDAARGDAVVAMDADLQDPPQ
                     LIGRLIEEWSRGADVVLARRTDRSADSFLKRTSAGLFYRIHNRLSSVQIPDNVGDFRL
                     IDRAAVEALKALPERQRFMKGLFAWIGFRTATVDYARRPRASGATKFSGWRLWNFALE
                     GITSFSTAPLRVWTYVGALGALLTFLYALFIIARVLILGVDVPGYASLLVAVLFFGSL
                     QLLSVGLLGEYIGRVYLETKQRPVYVVRREFGARDAP"
     gene            1178..1891
                     /locus_tag="VIG70_03025"
     CDS             1178..1891
                     /locus_tag="VIG70_03025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8249551.1"
                     /translation="MHPSAYREMADTEGRHWWFTGRRKILSSLISRLGLPERARIVEI
                     GCGTGGNLEMLSAFGELSAMETDDGARAIATEKAGPRVEIRAGRCPDDIRFAKGSFDL
                     VCLFDVLEHIEEDLATLQAVGALLAPGGLAVITVPAYRWMWSAHDEFLHHKRRYTAGE
                     LRKKIAASGLKARKLSYFNTLLFPLAATARLFKLAGTAVPAPPVNRLFHTVFSAERFV
                     VGRLPLPFGVSLLAVTGRS"
     gene            complement(1875..>2096)
                     /locus_tag="VIG70_03030"
     CDS             complement(1875..>2096)
                     /locus_tag="VIG70_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249552.1"
                     /translation="PDQLEQRPSGDPVRCVVTDLRMGLNWPLGRGGVERVLATLPERG
                     YRLAWRCGDFTVYELGSAGCMRCVPSCGR"
BASE COUNT          294 a          731 c          711 g          360 t
ORIGIN      
        1 cgccgccgcc gccgcgctgc tggtcccggt cggcgtgctg ctcggtgtcg gcctgccggg
       61 cggcatgcgc ttgctcaacc gcgcgcggcc cgagatcgtg gcctggggct ggggcatgaa
      121 cggcgcgttc tcggtgatcg gggcgaccgc ggcgatcttc atcgcgatga actgggggtt
      181 ctcggtcacg ctcctcgccg gcgccctcac ctacctcctg gccgccggct gcctgaaaat
      241 gtgccgatga aactcgtctc gctcgtcgtg ccgttccaca acgaggcggg cggggttgaa
      301 gcttttcatc gggaagtgtc aaccgctttg aacgccctgc ccgcgtaccg gttcgaaatc
      361 gtgtgcgtgg acgacggcag cagcgatgcc acgttgcagg ggcttctgca gctcgtcgag
      421 cgcgaccggc gtgtgcgcgt gctggagctc tcgcgcaact tcggcaagga agccgcgctg
      481 accgccggac ttgacgccgc ccgcggcgac gccgtggtcg cgatggacgc cgacctgcag
      541 gacccgccgc agctgatcgg gcggctgatc gaggaatggt cgcgcggcgc cgacgtagtg
      601 ctcgcgcggc gcaccgaccg cagcgccgac tccttcctca agcgcactag cgccggactc
      661 ttctaccgca tccacaaccg gctctcgagc gtgcagatcc cggacaacgt cggcgatttc
      721 cggctgatcg accgcgcggc ggtcgaggcg ctgaaggcgc ttcccgagcg ccagcgcttc
      781 atgaagggtc tgttcgcctg gatcggcttc cggaccgcga ctgtggatta cgcgcgccgc
      841 ccgcgcgcgt cgggcgcgac caagttctcc ggctggcggc tgtggaactt cgcgctcgaa
      901 ggcatcacca gcttcagcac cgcgccgctg cgggtctgga cctacgtcgg cgcgcttggc
      961 gcgctcctta cctttctgta cgcgctgttc atcatcgcgc gcgtcctgat cctcggcgtc
     1021 gacgtcccgg gctacgcttc gctgctcgtc gccgtcctgt tcttcggcag cctgcagctt
     1081 ctcagcgtgg ggctgctggg cgagtacatc gggcgcgtgt acctcgagac caagcagcgc
     1141 ccggtttacg tggtgcggcg ggagttcgga gcccgcgatg caccctagcg cctaccgtga
     1201 aatggcggac accgaaggcc gccattggtg gttcacggga aggcggaaaa tcttgtcgtc
     1261 gttgatctcc cggctcgggc tgcccgagcg ggcaagaatt gtggagatcg gctgcgggac
     1321 gggcggcaac ctggagatgc tctcggcctt cggcgagctg agcgccatgg aaaccgacga
     1381 cggcgcccgc gccatcgcca cggagaaagc cgggccgcgc gtggagatcc gcgcggggcg
     1441 ttgcccggac gacattcgct tcgccaaggg gagcttcgac ctggtctgcc tgttcgacgt
     1501 gctcgagcac atcgaggaag acctggctac cctgcaagcc gtcggcgccc tgctcgcgcc
     1561 cggcggcctt gcggtgatca ccgttccggc ctaccgctgg atgtggagcg cgcacgacga
     1621 gttcctgcac cataagcggc gctataccgc gggtgagctc cggaagaaga tcgcggcctc
     1681 ggggctcaaa gcccggaagc tgtcgtactt caacacgctg cttttcccgc tggcggcgac
     1741 agcgcggtta ttcaagctgg ccgggactgc ggtcccggcg ccgccggtca accgcctgtt
     1801 ccacacagtg ttcagcgccg agcgcttcgt cgtcgggcgc ctgccgctgc cgttcggcgt
     1861 ttcgctgctc gccgtcaccg gccgcagcta ggcacgcagc gcatgcagcc cgcgctcccc
     1921 agctcgtaca cggtgaaatc gccgcagcgc cacgccagtc ggtagccgcg ctcaggcaat
     1981 gtcgccagca cgcgttccac gccgccgcgg cccagcggcc agttgagccc cattctcaag
     2041 tcggtcacca cgcagcgcac cggatcgccg gacgggcgct gctcgagctg atcggg
//
LOCUS       DATBSZ010000046         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1030303, whole genome shotgun sequence.
ACCESSION   DATBSZ010000046 DATBSZ010000000
VERSION     DATBSZ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030303"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="VIG70_03035"
     CDS             <1..1593
                     /locus_tag="VIG70_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249553.1"
                     /translation="AAAGPSGAQEEQTPEGVPTFAELETAGARVGEIRVETHDIFDLD
                     DPRENNFLYRLANKLHINTRPEVVRRLLLFQTGEPVSVRLIEETERLLRGKNYLYEVS
                     IRPIAYHHGVVDIEVITRDTWSLDLGIGVSREGGANKGRLSIKEANVLGTGILLGLSY
                     SSDVDRSGTTFEIADNNLFGTRGTVAYTYAKYDDGNHQSFSLQRPFYALDARWAAGLS
                     ASSTDSIDALYNAGNNIAEYRHHRQAGNMFGGWSRGRIGRWTRRYSLGLAYEDNDYEL
                     EPGKTAPERLPSDLTLAGPFVRFELIEDAFRTDVNLNLIGRVEDFAMGVQANLQIGRA
                     LTEMGSTRDSWVYSAGVSNGFDITRDSFLLANLNAGGRYAEQAENQSISAAARYYHRQ
                     GRHLLYYAALSGDAVDNPDVPSPLQIGGDTGLRGYPLRYQGGERRVLFTTEARAYTDW
                     YPFRLFRVGGAVFYDTGRAWKGENQNTVNSGWLRDVGFGLRLLSARTSKGNVLHADVA
                     FPLDRDPSIDSVQFLVKTQVAF"
     gene            1761..2216
                     /locus_tag="VIG70_03040"
     CDS             1761..2216
                     /locus_tag="VIG70_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8249554.1"
                     /translation="MVSVTINGTARSFDGDADMPLLWYLRDVLQATGTKFGCGQALCG
                     ACTVHLDGAAVRSCQTPMKAVAGKAIVTIEGLDAKGQHPLQVAWRELGVPQCGYCQSG
                     QIMQAAALLKQKSAPSDADIDAAMSGNICRCGTYTRIKAAIKRAATKGA"
     gene            2219..>2461
                     /locus_tag="VIG70_03045"
     CDS             2219..>2461
                     /locus_tag="VIG70_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY8249555.1"
                     /translation="MGASELSRREFLKVTAAAGGFALSAGWTQVLAQEKYGSDSMPNG
                     AVDNPLVFVSIAANGIVTITCHRAEMGQGVRTGMPMI"
BASE COUNT          438 a          831 c          803 g          389 t
ORIGIN      
        1 gcggcggccg ggccgtccgg cgcgcaggag gagcagacgc ccgagggcgt tccgactttt
       61 gccgagctcg agaccgccgg ggcgcgcgtc ggggagatcc gcgtcgagac ccacgacatc
      121 ttcgacctcg acgacccgag agagaacaac ttcctctacc gcctggcgaa caagctgcac
      181 atcaataccc gccccgaggt cgtccggcgg ctgctgcttt tccagacggg ggaaccggtg
      241 tcggtgcgcc tcatcgagga aaccgagcgg ctgctgcgcg ggaagaatta cctctacgag
      301 gtgagcatcc ggccgatcgc gtaccaccac ggcgtggtag acatcgaagt catcacgcgc
      361 gatacctggt cgctggacct gggcatcggc gtgagccggg aaggcggcgc gaacaagggc
      421 cggctctcga taaaggaggc gaacgttctc ggcaccggca tcctgctggg gctctcctac
      481 agctccgacg tcgaccgaag cggcaccacg ttcgagatcg cggataacaa cctcttcggc
      541 acgcgcggaa ccgtcgccta tacgtacgcc aagtacgacg atggcaacca ccagtcgttt
      601 tcgctgcagc ggccctttta tgcgctcgat gcacgctggg cggcggggct cagcgcttcc
      661 tcgaccgata gcatcgacgc gctctacaac gcgggcaaca acatcgcgga ataccgccat
      721 catcgtcagg cgggcaacat gttcgggggc tggtcgcgag gtcgcattgg ccgctggaca
      781 cgccgttatt ccctcggact cgcgtacgag gacaacgact acgagctcga gccgggcaaa
      841 accgcgcccg agcgcctgcc gtcggacctc acgcttgcgg ggccgttcgt gcgcttcgag
      901 ctgatcgagg acgctttccg caccgacgtc aacctgaacc tgatcggacg cgtcgaggac
      961 ttcgcgatgg gcgtgcaggc gaacctgcag atcggccgcg cgctcactga aatgggctcg
     1021 acgcgcgact cctgggtcta tagcgccggc gtgagcaacg gcttcgacat cacccgagac
     1081 agcttcctgc tcgccaatct caacgccggc gggcgctatg ccgagcaagc cgagaatcag
     1141 tcgatcagcg cagcggcccg ctattaccac cgccagggca ggcatctgct gtactacgcc
     1201 gcgctctcgg gcgatgccgt cgacaacccc gatgtaccct ccccgctgca gatcggcggc
     1261 gacaccggct tgcgcggcta cccgctgcgc taccaaggcg gcgaacgccg ggtgcttttc
     1321 acgaccgaag cgcgcgcata cacggactgg tatccgtttc ggctgttccg cgtcggcggg
     1381 gccgtgttct acgacaccgg ccgcgcctgg aagggggaga accagaacac cgtcaactcc
     1441 ggctggctgc gggacgtggg tttcgggctg cgcctgctca gcgcacgcac ctccaagggc
     1501 aacgtgctgc acgccgacgt cgcgttcccg ctcgatcgcg acccgagcat cgactcggtg
     1561 cagttcctgg tgaagaccca ggtcgcgttc tagcgctcgg gcagggcggt cccatccgac
     1621 gcggttgccg catcgtgggc ggttgcccat cgccagaaca gatggctgcg ccgcgcggcc
     1681 gccgcatatt cgttgtcacc gtgaaggtcg aggactagac ttttccggcg gagcaatcac
     1741 ccccgccctg ggaggaaact atggtttcgg tcacgatcaa cggcaccgct cgctcgttcg
     1801 acggcgatgc ggacatgccc cttctctggt atctgcgcga tgtactgcag gcgaccggaa
     1861 cgaaattcgg ttgcgggcag gccttgtgcg gcgcctgcac ggttcatctc gacggcgccg
     1921 ccgtgcgctc gtgccaaacg ccaatgaagg cggtggccgg caaggcgatc gtcaccatcg
     1981 agggcctcga cgccaagggc cagcatccgc tgcaggtggc gtggcgcgag ctcggcgtgc
     2041 cgcagtgcgg ctactgccag tccgggcaga tcatgcaggc cgcggcgctg ctcaagcaga
     2101 aatccgcgcc cagcgacgcc gacatcgatg ccgcgatgtc cggcaacatc tgccgctgcg
     2161 gaacctacac gcgcatcaag gcggccatca agcgcgccgc gacgaaagga gcctgaccat
     2221 gggtgcgagc gaactttcgc ggcgcgaatt cctcaaggtc actgcggcag cgggcggttt
     2281 cgcgctgtcg gcgggctgga cgcaggtgct tgcgcaggag aaatacggct ccgacagcat
     2341 gccgaacggc gcggtcgaca acccgctcgt cttcgtgtcc atcgccgcca acggcatcgt
     2401 gaccatcacc tgccaccgcg cggaaatggg ccagggcgtg cgcaccggga tgccgatgat
     2461 c
//
LOCUS       DATBSZ010000047        10433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_620943, whole genome shotgun sequence.
ACCESSION   DATBSZ010000047 DATBSZ010000000
VERSION     DATBSZ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620943"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /gene="rpiA"
                     /locus_tag="VIG70_03050"
     CDS             <1..559
                     /gene="rpiA"
                     /locus_tag="VIG70_03050"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY8249556.1"
                     /translation="ALSSIKHKIDGAVAASEASAERLKKHGIRVLDLNSVNELPVYID
                     GADEVTEHLHMIKGGGGALTREKITAAVARKFVCICDGSKLVPVLGKFPLPVEVIPMA
                     RSYVAREIAKMGGHPRLREGFTTDNGNVVLDCHGMTLLDPPKTETELNQIAGVVTSGI
                     FARRAADVLLLAESAGVRILKKTLR"
     gene            560..1720
                     /locus_tag="VIG70_03055"
     CDS             560..1720
                     /locus_tag="VIG70_03055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8249557.1"
                     /translation="MGRAVFLLSLAAFASAASLRATDPLLPLIAGEHGVTTGAASAVV
                     TAFALAYGLLQVACGPLGDRYGRYRTVAAAAFVSAFGSAACAIAPSLDLLVAARFVSG
                     ATMGAFIPLALAWIGDTVAYEQRQPLLARFLVGQMAGVAFGTAAAGWLGEHFGWRTIF
                     FALAALLLVIGVLLLAELRNPLVRSVRRGSLTESFRRMPRMLAQRRLRILFATGFTEG
                     VFIFGALAFVAVHFQSRFALGPGAAGTLMAMYAAGGVVYALLARRAVRRLGERGLVTL
                     GGTALVLGYGALALASTVFIAVIGITAVGAGFYMLHTTVQMHVTQVAPEERGSAVALF
                     ATFLFLGQASGVWLAARVVDNAGVAPVFACAALGLGVLAVLFRYFLSGGGGT"
     gene            complement(1690..1971)
                     /locus_tag="VIG70_03060"
     CDS             complement(1690..1971)
                     /locus_tag="VIG70_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEY8249558.1"
                     /translation="MARMVKCVKLGREAEGLDFPPYPGALGKRIWESVSKEAWAQWLK
                     QQTMLVNENRLNLADQRARKYLMEQTERHFFGTGADVASGYVPPPPDKK"
     gene            complement(1979..3292)
                     /gene="argA"
                     /locus_tag="VIG70_03065"
     CDS             complement(1979..3292)
                     /gene="argA"
                     /locus_tag="VIG70_03065"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEY8249559.1"
                     /translation="MQSPESFVRWFRQVAPYVHDFGGRTFVIAFGGEMVAERARFESF
                     IHDVNVLASLEIRLVLVHGARPQIEAEMKAKGLRSRYAQGLRVTDEPALTAVKHASGV
                     LRVEIEALLSQGLPDSPMARAQIRVASGNYITARPIGVRNGVDFLFTGAVRKVDAAAL
                     AAQLDVTDIVLVPPLGYSATGEVFNLAWEDVAESVAVALRADKLLMYTDRLPTDRKGE
                     VVSELTADQAATLATKKGTLTPAATRALEHLARAVEGGVARGHLVTRRAPGALLLELF
                     THAGVGTMVTADAMEKMRPARIEDVRGMLALIEPLEADGTLVKRSRELLESEIGNFLV
                     VEHDARIVGCAALYPFPDDKSAEFACLAVANEYRDAGYGERLLKACEERARGLRLRKL
                     FALTTHAAHWFLEQGFRAGAVDALPDRRKELYNWRRGSKVFLKRI"
     gene            3378..3971
                     /locus_tag="VIG70_03070"
     CDS             3378..3971
                     /locus_tag="VIG70_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249560.1"
                     /translation="MKPIRTLLVAILLVPALPVKAAEESPATSLNHMLTLVHMFVRIA
                     AESDSSQASLRAIDEVLSGRNVAANEAVAGLMKDALADVSPENRATIAAIGRDLVGVA
                     RKNLLGAGPGQALFPDRSLDARKELTAMGLTYHDSKQFLDAVKRNDTMAAELFIAGRG
                     VDLSATDFWGRDAVDFARRNSNERLAELILRSRPAAR"
     gene            complement(3946..4668)
                     /locus_tag="VIG70_03075"
     CDS             complement(3946..4668)
                     /locus_tag="VIG70_03075"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY8249561.1"
                     /translation="MVPRFYVDAPLRAGSVCTLSEDAAHHAIHVLRLREGEDVTLFNG
                     RGGEFAARIASIQRLKIAIDLLQHRPIEREAPLRVTLVQGVSAGEKMDGTVRKAVELG
                     VAAVQPVLGARSVARPKGDRADSRRGHWQKVVIAACEQCGRNRIPEVHPLIAVSDYRA
                     PGPGMKILLSPLAGLPLSKISFQGNDFTVAAGPEAGFTPEEEAALASAGFVPASLGPR
                     VLRTETAAVAALAALSALQGDS"
     gene            4683..5162
                     /locus_tag="VIG70_03080"
     CDS             4683..5162
                     /locus_tag="VIG70_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY8249562.1"
                     /translation="MKAAQFELLGVDGKRYSPATARGDKGLLVMFICNHCPYVKRVID
                     GIVRDCKALAPLGIGSIAVMSNDPSDYPEDSFDNMKRVAREKGFPFPYVVDETQDVAR
                     AYGTVCTPEFFGFDARLELQYHGRFDELFKAMKQIAETGKGPEKQVPSVGCSIKWKK"
     gene            complement(5229..6749)
                     /locus_tag="VIG70_03085"
     CDS             complement(5229..6749)
                     /locus_tag="VIG70_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEY8249563.1"
                     /translation="MLQAVELTIRLHLDDDVVIARLELATGTLVSRENVRTAVTIPAG
                     HKLAVRDVAQGTPVQRYNQIIGFATRDIKAGEHVHVHNLAMGNFQRDYAFSSLVKNTE
                     YFEKPATFLGIVRADGRVATRNFIGILTSVNCSATVAKMIARHFENNLSQYPNVDGVV
                     ALTHKTGCGMASEGEGMDVLRRTMAGYARHPNFFATQVIGLGCEANQINNLLSTQSLK
                     RHDRLHAFTIQEKGGTMKAVREGIARIEAILPEANKVKRETVPASHLTLGLQCGGSDG
                     YSGISANPALGAAVDLLVRHGGAAILSETPENYGAEHLLTRRAVSKEVGEKLIRRIKW
                     WEEYTARNNMEMNNNPSPGNKAGGLTTILEKSLGAVAKGGTTNLVDVYEYAERVLAKG
                     FVYMDTPGFDPVSATGQVAGGANMICFTTGRGSAYGCKPSPSLKLATNTPLFVHQEED
                     MDFNCGTIIDGTETIAQAGERFFQLILETASGKKSKSEEFGYGDDEFAPWTIGATM"
     gene            complement(6753..7379)
                     /locus_tag="VIG70_03090"
     CDS             complement(6753..7379)
                     /locus_tag="VIG70_03090"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY8249564.1"
                     /translation="MTTTEFRLYCFAQSGNAYKVALMLNLCRAKWEPRFVDYFAGETR
                     TPEYRAINEMGEVPVLEHGAKRLSQSGVILHYLADHFGKFQGNDRLEVLRWMLWDNHK
                     LTSYIATLRWMVTLAKVGEPQVHEFLRGRVKTALVILNSHLEKGAFVVGNQPSIADIS
                     MCGYLYWSEEFGVSWSEYPRIGAWLDRIRALPGWVHPYDLMPGHPLKR"
     gene            complement(7366..9102)
                     /locus_tag="VIG70_03095"
     CDS             complement(7366..9102)
                     /locus_tag="VIG70_03095"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEY8249565.1"
                     /translation="MKKTRKENAWRSIQWFGRQDIYGFIYRSWVKNRGVPQDQFDGRP
                     VIGICNTWSELTPCNTHFRVLAEHVRNGVLEAGGFPLEFPVMSLGETLMRPTAMLYRN
                     LAAMSVEESIRANPLDAVVLLAGCDKTTPATLMGAASVDLPTILVSGGPMLSGKYRDT
                     DIGSGTNTWSMSEDLRAGKITLDEFHEAESCMHRSHGHCMTMGTASTMASMMEALGVA
                     MPGNAAIPAVDARRNVLARMAGRQIVQLVKSNTTLSKILVKPAFENAIRALAAIGGST
                     NAVIHLTAIARRIGVELGLKDFARLGRDVHTLVNLMPSGKYLMEDFYYAGGLPAVLRE
                     LGENNLLNKSALTVNGKTQWENVKDAPCWNREVITRFKKPFKKNTGIAVLRGNLCPDG
                     AIIKPSAATPRLLKHKGRAVVFESIEDFHARIDDPSLDIDERCVMVLKNCGPKGYPGM
                     AEVGNMPLPPKLLKKGITDMVRISDARMSGTAYGTVILHISPEAAAGGTLALVENGDE
                     IELDVAKGRLNLRVSPKELARRKKSWKKPEAPLPRGYWKLYFDHVQQASDGADLDFLV
                     GKSGAFVPRDNH"
     gene            complement(9098..9844)
                     /locus_tag="VIG70_03100"
     CDS             complement(9098..9844)
                     /locus_tag="VIG70_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8249566.1"
                     /translation="MNKLDFAGRNAIVTGGAQGIGLAIVSRLVASGAQVRIWDRDDKL
                     LAKTVAKLGKSVTGDALDVTDAGAIERGTKSALDALGRIDVLVNNAGIAGPSKPVVDY
                     PVDEWKRVIDIDLTGPFLCCRAVVPHMTRAGYGRIVNIASVAGKEGNPNAAAYGAAKG
                     GLIAFTKSLGKELAQSGVLVNCVTPAAAQTAILDQVTDEFAKYMLSKIPMGRFVKVEE
                     IAALACWLASEDCSFSTAGVFDISGGRATY"
     gene            9888..>10433
                     /locus_tag="VIG70_03105"
     CDS             9888..>10433
                     /locus_tag="VIG70_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8249567.1"
                     /translation="MPRLAGKTAFITAAGQGMGRAAAVAFAREGAKVWATDMNAKTVG
                     ELEGKDGIRARALDVTDENSIKKIAGEVGEIDVLFNCAGIVHHGSILDATPKDWDQAF
                     AVNVKSMYLVSRAFLPGMLKKGRGSIINMASVASSIRGLPNRFVYGASKAAVIGLTKA
                     IAADYVGKGIRCNAICPGTVDT"
BASE COUNT         1594 a         3424 c         3559 g         1856 t
ORIGIN      
        1 ggctctttcc tcgataaagc acaagattga cggcgcggtg gccgcctccg aggcgagcgc
       61 cgagcggctg aagaagcacg gcatccgcgt gctcgacctg aacagcgtga acgagcttcc
      121 ggtgtacatc gacggcgccg acgaggtcac cgagcacctg cacatgatca agggcggcgg
      181 cggcgcgctg acgcgcgaga agatcaccgc cgcggtggcg cgcaagttcg tgtgcatctg
      241 cgacggatcc aagctggtgc cggtgctcgg caagttcccg ctgccggtcg aggtgatccc
      301 gatggcgcgc agctacgttg cgcgcgagat cgcgaagatg ggcggccacc cgcggctgcg
      361 cgagggcttc accaccgaca acggcaacgt ggtgctcgac tgccacggca tgacgctgct
      421 cgacccgccg aagaccgaga ccgagctgaa ccagatcgcc ggcgtggtga cgagcgggat
      481 cttcgcccgc cgcgcggccg atgtgctcct gctcgcagaa tctgccggtg taaggattct
      541 caagaagaca ctgcgttaat tgggccgagc ggtatttcta ctgtccctgg ccgcgttcgc
      601 cagcgccgcg agcctgcgcg ccaccgaccc gctgctgccg ctgatcgccg gcgagcacgg
      661 cgtcacgacc ggcgcggcgt ccgcggtcgt gaccgcattc gccctcgcct acgggctgct
      721 gcaggtcgcc tgcggcccgc tcggcgaccg ctacgggcgc taccgcaccg tcgccgcggc
      781 ggctttcgta tcggcgtttg gctccgcagc ttgcgcgatc gcgccttcgc tcgacctgct
      841 ggtcgctgcg cgcttcgtgt ccggggcgac gatgggcgcc ttcatcccgc tcgcgctcgc
      901 ctggatcggc gacaccgtgg cgtacgagca gcgccagccg ctgctcgcgc gcttcctcgt
      961 cggccagatg gccggcgtag cgttcggcac cgcggccgcc gggtggctgg gcgagcactt
     1021 cggctggcgc acgatcttct tcgcgctcgc tgcgctgcta ctggtcatcg gtgtcctgct
     1081 tctcgccgag ctgagaaacc cgctcgtgcg cagcgtgcgg cgcgggtcgc tcacggagag
     1141 cttcaggcgc atgccccgca tgctggcgca gcgccggctg cggatcctgt tcgccaccgg
     1201 cttcaccgag ggcgtgttca tcttcggcgc gctcgcgttc gtcgcggtcc acttccagag
     1261 ccgcttcgcc ctcggcccgg gcgccgccgg cacgctgatg gcgatgtacg ccgcgggcgg
     1321 ggtggtgtac gcgctgctcg cgcgacgcgc ggtgcggcga ctgggggagc gaggcctggt
     1381 cactttgggc ggcacggcac tcgtgctcgg atacggtgcg ctcgcgctcg cctctaccgt
     1441 gttcatagcg gtgataggta tcaccgcggt cggagcgggg ttctacatgc tgcacacgac
     1501 ggtgcagatg cacgtcacgc aggtcgcgcc ggaagaacgc ggctcggcgg tggcgctgtt
     1561 cgcgacgttc ctcttcctgg ggcaggcgtc gggagtgtgg ctcgcggcgc gcgtggtcga
     1621 taacgccggc gtggcgccgg tgttcgcctg cgccgcgctc gggctcggcg tcctggcggt
     1681 tctcttccgc tacttcttgt cgggcggcgg cgggacgtag cccgaggcga cgtcggcgcc
     1741 ggtgccgaag aaatgccgct cggtctgctc catgaggtac ttgcgcgcgc gctggtcggc
     1801 gaggttgagg cggttctcgt tcaccagcat ggtctgctgc ttgagccact gcgcccacgc
     1861 ctctttcgag acgctctccc agatgcgctt gcccagagcg cccggatacg gggggaaatc
     1921 gagcccttcg gcctcgcggc cgagcttcac gcacttcacc attcgcgcca tcgggaaatt
     1981 atattcgctt gaggaagact ttcgagccgc ggcgccagtt gtagagctcc ttgcggcgat
     2041 ccggcagcgc atccacggcg ccggcgcgga agccctgctc gaggaaccag tgggcggcgt
     2101 gcgtggtgag cgcgaagagc ttgcgcaggc gcaggcccct ggcgcgctcc tcgcacgcct
     2161 tgaggagccg ctcgccgtag cccgcgtcgc ggtattcgtt cgccaccgcc aggcaggcga
     2221 actcggcgct cttgtcgtcg ggaaagggat agagcgcggc gcagccgacg atgcgcgcgt
     2281 cgtgctcgac gaccaggaag ttgccgatct cggactccag cagctcgcgg ctcctcttca
     2341 ccagcgtgcc gtcggcttcg agcggctcga tcagggcgag catgccgcgc acgtcctcga
     2401 tgcgcgccgg gcgcatcttt tccatggcgt cggcggtgac catggtgccg acgccggcat
     2461 gcgtgaagag ctcgaggagc agggcgccag gcgcgcggcg cgtgactagg tggccgcgcg
     2521 ccacgccgcc ttcgacagcg cgcgcgagat gttccagggc tcgggtcgcg gccggcgtca
     2581 gggttccctt cttcgtcgca agcgtggcgg cctgatcggc ggtgagttcc gacaccactt
     2641 cacccttgcg gtcggtcggc agccggtcgg tgtacatgag gagcttgtcg gccctgagcg
     2701 ccacggcgac gctctcggcg acatcctccc aggcgaggtt gaacacttcg ccggtcgccg
     2761 agtagccgag gggcggcacc agcacgatgt cggtgacgtc gagttgggcg gcgagcgcgg
     2821 cggcgtccac cttgcgcacc gcgccggtga acaggaagtc gacgccgttg cgcacgccga
     2881 tcggccgcgc ggtgatgtag ttcccggaag ccacccggat ctgggcgcgc gccatcggcg
     2941 agtcgggcag gccctgcgag agcagcgcct cgatctccac gcgcagcacg ccggaggcgt
     3001 gcttgaccgc ggtcagcgcc ggctcgtcgg tgacgcgcag cccctgggcg tagcgcgagc
     3061 gcaggccctt cgccttcatc tcggcctcga tctgcggccg ggcgccgtgc accagcacca
     3121 ggcgaatctc gagcgaggcg agcacgttga catcgtggat gaagctctcg aagcgcgctc
     3181 gctcggcgac catttcgccg ccgaaggcga tgacgaacgt gcgcccgccg aagtcgtgca
     3241 cgtacggcgc gacctggcgg aaccagcgga cgaagctctc ggggctctgc atgcgtgtaa
     3301 ttttttcaaa ttctacttcg gtgaaccatt ttccgtttgg cgcgttatcc tttggatcca
     3361 ccgaggggag cttcaccatg aaaccgattc gcactttgct cgttgccatc ctgctggttc
     3421 ccgcgctgcc ggtgaaggcc gccgaggagt ccccggcgac ctcgctgaac cacatgctga
     3481 cgctggtgca catgttcgtg cgcatcgcag ccgagtcgga cagctcgcag gccagcctgc
     3541 gggcgatcga cgaggtgctg agcgggcgca acgtcgccgc caacgaggcg gtcgcgggcc
     3601 tgatgaaaga cgccctggcc gacgtctcgc ccgagaaccg ggccaccatc gccgccatcg
     3661 gccgcgacct ggtcggcgtg gcgcgcaaga acctgctcgg cgcgggcccc gggcaagcgc
     3721 tgtttcccga tcgctcgctc gatgcgcgca aggagctcac cgcgatgggg ctcacctacc
     3781 acgactcgaa gcagttcctc gacgcggtca agcgcaacga cacgatggcg gccgagctct
     3841 tcatcgccgg gcgcggcgtg gatctttccg cgaccgattt ctggggccgc gacgccgtcg
     3901 acttcgcgcg ccgcaacagc aacgagcgcc tcgccgagct gattctcagg agtcgccctg
     3961 cagcgcgctg agggcggcga gcgcggcgac cgccgcggtc tcggtgcgca gcacccgcgg
     4021 tccgagcgac gccgggacga aaccggcgct cgcgagggcc gcttcttctt ctggcgtgaa
     4081 gcccgcctcg ggcccggccg cgacagtgaa atcgtttccc tgaaaagaga tcttggatag
     4141 cggcagcccg gcgagcgggg atagcaggat cttcatgccc ggcccgggag cccggtagtc
     4201 cgaaaccgcg atcaggggat gcacctcggg gatgcggttg cggccgcact gctcgcaggc
     4261 ggcgatgacg accttctgcc agtgaccgcg gcggctgtcg gcgcgatcgc ctttcggccg
     4321 cgcgaccgag cgcgccccca ggaccggctg gactgcggcc acgccgagct cgaccgcctt
     4381 gcgcaccgtg ccgtccatct tctcgccggc cgacacgccc tgcaccagcg tgacgcggag
     4441 cggcgcctcg cgctcgatcg ggcgatgctg caacaggtcg atcgcgatct tcagccgctg
     4501 gatcgaggcg atgcgcgccg cgaactcacc gccgcggccg ttgaacagcg tgacgtcctc
     4561 gccctcgcgc agtcgcagca cgtggatcgc gtgatgcgcc gcgtcctccg agagcgtgca
     4621 cacgctgccc gcgcgcagcg gggcatcgac gtagaaacgc gggaccatgc taggattttt
     4681 gcatgaaggc ggctcaattc gagcttctcg gagtcgacgg caagcgctac tcgccggcca
     4741 cggcgcgcgg cgacaagggc ctgctggtga tgttcatctg taaccactgc ccgtacgtga
     4801 agcgcgtgat cgacggcatc gtgcgcgact gcaaggcgct ggcgccgctc ggtatcggct
     4861 cgatcgcggt catgtccaac gacccttccg actatccgga agactcgttc gacaacatga
     4921 agcgcgtggc gcgcgagaag ggcttcccgt ttccctacgt cgtcgacgag acgcaggacg
     4981 tggcgcgggc ttacggcacg gtgtgcacgc cggagttctt cggcttcgac gccaggctgg
     5041 agctgcagta ccacggccgg ttcgacgagc tgttcaaggc gatgaagcag atcgccgaga
     5101 cgggcaaggg accggagaag caggtgccct cggtcggctg ctcgatcaag tggaaaaaat
     5161 gaattcgggg acggagccct aactccacgg ctggcgcagc tttcggagtt agggctccgt
     5221 ccccgaattc acatcgtcgc gccgatagtc cacggcgcga attcgtcgtc cccgtagccg
     5281 aactcctcgc tcttcgattt ctttccggac gccgtctcga gaatcagctg gaagaaccgc
     5341 tcgccggctt gcgcgatggt ctcggtgccg tcgatgatcg tgccgcagtt gaagtccatg
     5401 tcctcttcct ggtgcacgaa gagcggcgtg ttggtggcga gcttgagcga gggtgagggc
     5461 ttgcagccgt aggccgagcc gcggccggta gtgaagcaga tcatgttggc gccacccgcg
     5521 acctggccgg tggccgacac cgggtcgaag cccggcgtgt ccatgtagac gaaacctttc
     5581 gccaacaccc gctcggcgta ctcgtagacg tcgaccagat tggtggtgcc gcccttcgcc
     5641 accgcgccga gcgatttctc gaggatcgtg gtgaggccgc cggccttgtt gcctggtgaa
     5701 gggttgttgt tcatctccat gttgttgcgg gcggtgtact cctcccacca cttgatgcgg
     5761 cggatgagct tctcgccgac ttccttcgac accgccctgc gggtgagcag gtgctcggcg
     5821 ccgtagtttt ccggggtttc ggacaggatc gccgctcctc cgtgccggac cagaagatcg
     5881 actgcggcgc ccagggcggg gtttgcgctg atgccggagt agccgtcgga gccgccgcac
     5941 tgcagcccga gcgtgagatg gcttgccggc accgtctcgc gcttcacctt gttcgcctcg
     6001 ggcaggatcg cctcgatgcg ggcgatgccc tcgcgcaccg ccttcatcgt gccgcccttc
     6061 tcctggatgg tgaaggcgtg caggcggtcg tggcgcttca ggctctgcgt ggaaagcagg
     6121 ttgttgatct ggttcgcctc gcagccgagg ccgatgacct gcgtggcgaa gaagttcgga
     6181 tggcgcgcat agcccgccat ggtgcggcga agcacatcca tgccttcgcc ttccgaggcc
     6241 atgccgcagc cggtcttgtg agtgagggca acgacgccgt cgacgttcgg gtactgactt
     6301 aaattatttt caaaatgccg ggcaatcatc ttggccacgg tcgccgagca gttcacgctg
     6361 gtgaggatgc cgatgaagtt gcgagtggcg acgcggccgt cggctcggac gataccgagg
     6421 aaagttgccg gtttttcgaa gtactcagta tttttgacta aagaagaaaa agcgtagtcg
     6481 cgctggaaat ttcccatcgc caggttgtgc acatggacgt gctcgccggc tttgatgtcg
     6541 cgcgtggcga agccgatgat ctggttatag cgctgcaccg gtgttccctg cgcgacgtcg
     6601 cgcaccgcga gcttgtgccc ggccggaatg gtgaccgcgg tgcgcacgtt ctcgcggctc
     6661 accagggtgc cggtggcgag ctccaggcgg gcgatgacga cgtcgtcgtc gaggtgcagg
     6721 cggatggtga gttcgacggc ttgcagcatg ggctacctct tcaacggatg gccgggcatc
     6781 aggtcgtagg gatgcaccca gccgggaagc gcgcggatcc ggtcgagcca ggcgccgatg
     6841 cgcgggtatt cgctccacga tacgccgaat tcctccgacc agtacaggta cccgcacatc
     6901 gagatgtcgg cgatcgaggg ctggttgccg accacgaacg cgcctttctc gagatgggaa
     6961 ttcaggatca ccagcgcggt cttgactcgc ccgcgcagga actcgtgcac ctgcggctcg
     7021 ccgaccttgg cgagcgtgac catccagcgc agcgtggcga tatagctggt gagcttgtgg
     7081 ttgtcccaca gcatccagcg caggacttcc agcctgtcgt tgccctggaa ttttccgaaa
     7141 tggtcggcga ggtagtgcag gatcacgccg gactgggaca ggcgcttcgc cccgtgctcg
     7201 agcaccggca cctcgcccat ctcgttgatc gcgcggtact cgggggtgcg cgtctcgccg
     7261 gcgaagtagt cgacgaagcg cggctcccac ttcgccctgc acaagttcag catgagcgcg
     7321 actttgtacg cgttcccgga ttgggcaaag caatacagcc ggaattcagt ggttgtcacg
     7381 gggcacgaag gcgccgctct tgccgacgag gaagtcgagg tccgcgccgt cgctcgcctg
     7441 ctgcacgtgg tcgaagtaga gcttccagta gccgcgtggc agcggcgcct cgggcttttt
     7501 ccagctcttc ttccggcgcg ccagctcttt cggggaaacc cgcaggttca gcctgccctt
     7561 cgctacgtcg agctcgatct catcgccatt ctcgaccagc gccagcgtgc cgccggcggc
     7621 agcttcgggc gaaatgtgca ggatgaccgt gccgtaggcg gtgccgctca tgcgcgcgtc
     7681 cgagatgcgc accatgtcgg tgatgccctt cttcagcagc ttcggcggca gcggcatgtt
     7741 gccgacttcg gccatgccgg ggtaaccctt cgggccgcag ttcttcagca ccatcacgca
     7801 gcgctcgtcg atgtcgaggc tgggatcgtc gatgcgcgca tggaagtcct cgatgctttc
     7861 gaacaccacc gcgcggccct tgtgcttgag gagccggggc gtcgccgccg agggcttgat
     7921 gatggcgccg tcggggcaca ggttgccgcg cagcacggcg atgccggtgt tcttcttgaa
     7981 cggcttcttg aaccgggtga tgacttcgcg gttccagcag ggcgcgtcct tgacgttctc
     8041 ccactgcgtc ttgccgttga cggtgagagc gctcttgttc aggaggttgt tttcgccaag
     8101 ctcccgcagg acggcgggaa gccctccggc gtagtagaag tcttccatga ggtacttgcc
     8161 cgacggcatg aggttcacca gcgtgtgcac gtcgcggccg aggcgggcga agtccttgag
     8221 gccgagctcg acgccgatgc gccgcgcaat ggccgtaagg tgaatcaccg cgttggtcga
     8281 gcccccgatc gcggcaagcg cgcggatcgc gttttcgaac gccggcttca ccaatatctt
     8341 cgagagagtg gtattgcttt ttactagctg aacgatctgt ctccccgcca tccgcgccag
     8401 cacattgcgg cgcgcgtcca ccgcgggaat cgcggcgttg cccggcatgg cgacgccgag
     8461 cgcctccatc atcgatgcca tggtcgaggc ggtgcccatg gtcatgcagt gtccgtgcga
     8521 gcggtgcatg caggactcgg cctcgtggaa ttcgtccagc gtgatcttgc cggcgcgcag
     8581 gtcctccgac atcgaccagg tgttggtccc cgagccgatg tcggtgtcgc gatacttgcc
     8641 ggagagcatc gggccgcccg agaccaggat cgtcggcagg tcgacgctcg ccgcgcccat
     8701 cagcgtggcc ggggtcgtct tgtcgcagcc ggcgagcagc accaccgcgt cgagcgggtt
     8761 ggcacggatc gattcctcca cgctcatcgc cgcgaggttc cggtacagca tcgcggtggg
     8821 gcgcatcagc gtctcgccga gcgacatcac cgggaattcg agcgggaagc cgccggcctc
     8881 gaggacgccg ttgcgcacat gctcggcgag cacgcggaag tgggtgttgc agggggtgag
     8941 ctcggaccag gtgttgcaga tgccgatgac ggggcggccg tcgaactggt cctgcggtac
     9001 gccgcggttc ttgacccagc tccggtagat gaagccgtag atgtcctgcc gcccgaacca
     9061 ctgaatcgag cgccaggcgt tttccttccg ggtcttctta ataggtcgcc cttcctccag
     9121 aaatgtcgaa aaccccggcg gtggagaagc tgcagtcctc gctcgcgagc cagcacgcga
     9181 gcgccgcgat ctcctccacc ttgacgaagc gccccatcgg gatcttcgac agcatgtact
     9241 tcgcgaattc gtcggtgacc tggtcgagga tcgcggtctg cgccgccgcg ggggtgacgc
     9301 agttgaccag cacgccgctc tgcgcaagct ccttgccgag cgacttggtg aacgcgatca
     9361 gcccgccctt ggccgcgccg taggcggcgg cgttcggatt gccttccttg ccggcgaccg
     9421 acgcgatatt gacgatgcgg ccgtacccgg cgcgcgtcat gtgcggcacg acggcgcggc
     9481 agcagaggaa cggcccggtg aggtcgatgt cgatgacccg cttccactcg tcgaccgggt
     9541 agtcgaccac cggcttgctc ggcccggcga tgccggcgtt gttgaccagc acgtcgatcc
     9601 tgccgagcgc atcgagcgcg ctcttcgtgc cgcgctcgat ggcgccggcg tcggtgacgt
     9661 cgagcgcatc gccggtgacg gacttgccga gcttcgccac cgtcttggcg aggagcttgt
     9721 catcgcgatc ccagatgcgg acctgcgcgc cggaggcgac gagacggctc acgatggcga
     9781 ggccgatgcc ttgcgcgccg ccggtcacga tcgcgtttcg gcccgcgaaa tcgagtttat
     9841 tcatgggaac gcgaagtata atcgcggcct tccatgcagg ccctcatttg cctcgcttag
     9901 ctgggaaaac cgccttcatc accgccgccg ggcagggcat gggccgcgcc gccgccgtcg
     9961 cctttgcgcg cgagggcgcg aaggtatggg ccaccgacat gaacgccaag accgtgggcg
    10021 agctcgaggg caaggacggc atccgagctc gggcgctcga tgtaaccgat gagaattcaa
    10081 taaagaaaat tgcaggcgaa gtcggcgaga tcgacgtgct gttcaactgc gcgggcatcg
    10141 tgcaccacgg ctcgatcctc gacgccacgc cgaaggactg ggaccaggcg ttcgcggtga
    10201 acgtgaagtc gatgtatctc gtgtcgcgcg ctttcctgcc cggaatgctg aagaaggggc
    10261 gcggctcgat catcaacatg gcctcggtcg cgtcctcgat ccgcggcctg ccgaaccgct
    10321 tcgtctacgg cgcgagcaag gccgcggtga tcggcttgac caaggcgatc gccgccgact
    10381 acgtaggcaa ggggatccgc tgcaacgcca tctgcccggg cacggtcgac acc
//
LOCUS       DATBSZ010000048        11682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2123682, whole genome shotgun sequence.
ACCESSION   DATBSZ010000048 DATBSZ010000000
VERSION     DATBSZ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2123682"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VIG70_03110"
     CDS             <1..427
                     /locus_tag="VIG70_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8249568.1"
                     /translation="AHGRLYFEERGWGERFEALVAGIAKDFIEKFDPSRERCWIAEMD
                     GEPVGCVFAVKESKTVAKLRLLLVEPKARGRGLGRRLVEECIAFARAKGYRKLVLWTQ
                     SNLTAARAIYRKTGFTKVKEEKHASFGVRLNGEYWELTL"
     gene            complement(506..1570)
                     /locus_tag="VIG70_03115"
     CDS             complement(506..1570)
                     /locus_tag="VIG70_03115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8249569.1"
                     /translation="MAEQEQRVSISIEQEELRGISRTVAEIHWLLLILVLLYLVFGGE
                     RGDPEADAAVSAGLFFYAAMVMSFRYANFYKRETRWKIAIETLGMIAFITWVLWYTER
                     LASPLVNLYLLPVITSSLTLGKLATLANVGLVASCYILLGSATMDEVLTLRFIAGFAG
                     QLAPVLLVAYITTMFSADIRYGLQQAKLLSETDELTGLYNTRGFAIAANRLFGQAVRY
                     GRPASLLMVDCDNLKPVNDSHGHEAGNRLLRQVANAVQSELRATDVPARYGGDEFIVL
                     LPETPPKGAADVAERIRNAIGARPIAFDGQRITATVSIGIACYPEDGRTLDALASRAD
                     RALYQAKQEGRNKVIRYKPV"
     gene            1608..2381
                     /locus_tag="VIG70_03120"
     CDS             1608..2381
                     /locus_tag="VIG70_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8249570.1"
                     /translation="MEISAKGLRVLVTAGAAGIGRVITRTFVEHGARVHICDVDEKAL
                     AEIKRELPSVSQTAADVARIDDVERLFEDVKKVLRGLDVLVNNAGIAGPTAKIEDIRP
                     EDWDRCIAVDLNGMFYCTRKAMPLIKAAGGGSIINLSSAAGRLAFPMRTPYSAAKWAI
                     VGFTESLAAEAGPDKVRVNCIQPGIVEGERIDRVIAAKAKALGVPEGEVKSKMVEGVS
                     MKTTVTQQDIADMALYLATAGRHISGQAISVCGGTRYLV"
     gene            2440..3375
                     /locus_tag="VIG70_03125"
     CDS             2440..3375
                     /locus_tag="VIG70_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY8249571.1"
                     /translation="MAAAQGKVIITCAVTGSIHTPSMSPHLPVTPAEVARGAIGAAQA
                     GAAIVHLHARNPKDGSPSQDPALFHEFLPQIAVAADVVINLTTGGAATMTTEERLQPA
                     LQLKPEVASLNMGSMNFGLYEMLGRYKEFKYDWEKPYLAGSDDRIFKNTFKDIAYILQ
                     SCSENGTRFEIECYDIGHLYTAAHFVDRGLIKPPMLIQSVFGIRGGIGPHPEDVMHMK
                     RTADRLFGDAYYWSVLGAGRNQMFIAAMSAVMGGNVRVGLEDSLWLGRGQLAKSNAEQ
                     VAKAKRMLEELGLSVATANDAREMLKLKGGRNVAF"
     gene            3362..4309
                     /locus_tag="VIG70_03130"
     CDS             3362..4309
                     /locus_tag="VIG70_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249572.1"
                     /translation="MSLFRFVLLLAFSLPVLAQDWPNKPVKFVSPYPPGGSVDPLARM
                     LGAKLGESLKQNFIVESRTGASGVIGTDYVAKSAPDGYTFVFIFDTHAVHQALNPKLP
                     FDPLKDFAPVMIVGTAPMAITTGTQKPYRNFQDVLKVAKSGGVSLGNVGNGSLAHLTT
                     IVLNQAAGTKFEPVAYKGGGPLSTDLIGGHVELAMASTAAQAQHVRSGKMRALVLTGD
                     KRSHTMPDVPTLKELGIDVVAHAWWGILAPAGTPKPIIDKIHAELVKAIKLPDVNKML
                     TETQGMDVVALSPEATRKWIADNMARWGKVVKDNDIRAE"
     gene            4306..5253
                     /locus_tag="VIG70_03135"
     CDS             4306..5253
                     /locus_tag="VIG70_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEY8249573.1"
                     /translation="MKLAVLDDYQRLALEYADWKSLKDVEVAVFDEPLKDAAQQLAPF
                     DIVCLMRERTPFPRALIEKLPNLKFVSLTGARSPSLDTQALKERGIPVSNTRSAGGGL
                     STVELTWGLIIAAARGLAKGERNMRAGRWHEGLSPGFSLAGKRLGLLGLGNIGARVAA
                     IGSAFGMECAAWSANLTAEKAAAAGASLVSKERLFAESNVVTIHLVLGDRTRGLVGAA
                     DLARMKPGAVLVNTSRGPIVVENDLLAALKAGKIQAALDVYEHEPLPAGHPLRKMENV
                     TLSPHLGYVHEDNYRIFYRDTAENVAAWLAGKPIRVINP"
     gene            5250..6704
                     /locus_tag="VIG70_03140"
     CDS             5250..6704
                     /locus_tag="VIG70_03140"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249574.1"
                     /translation="MRLSYLPPLMVYAAYGVSGLTGIVGTFFVKDYLELSPEFLAALL
                     FWAGIPWALKMPVGHVVDLIWRWKAALVWLGASMIAASLLIMIGLISHTEAMRSVMST
                     GSWFVLSSLLAPTGYMIQDSVADAMTVEAVPRVDDPKLQKSMHTTMQTLGRVALIGGL
                     TLVAAANVYIFHDAEALPKEQKAALYADVYTAALVVPVLSVLGVILHRFLKHEREVVE
                     KTRPNWAVLGGSLAFVAVTLAVGVGGVANAEEIIFAGSLAVILFLLWRLARELEPAAR
                     NVLVATAVVLFLYRAVPLTGDGSTWWMIDVLKFNEAFLAQLSLVTYALTLVGMFALRR
                     FMAEHSIATIVVVLSVAAAALALPTIGMFYGLHEWTAAHTGGRVDARIIMLVNTALES
                     PLGQIAMIPMLAWIANSAPAHLKATFFAVMASFSNLALAASQLGTVYLNRVFVVKRGD
                     YSELGILMVVAAVLTLALPLIAVAGVRLLRLRTA"
     gene            6770..7609
                     /locus_tag="VIG70_03145"
     CDS             6770..7609
                     /locus_tag="VIG70_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8249575.1"
                     /translation="MKRRRILQSLAAVLVAQAWGVPAWASAYPRTAAVPGGVARIRLG
                     AAERAPRVLLEGERVLVVREDGEWVALVGIALTARPGSKVRLEVEHADGKRKQLEVKV
                     AAKAYASQHLKVPPGQVELSAEDLARHERERTHLREVLRTFTDSAPATLAMLQPAPGR
                     RSSVFGLQRYFNGQLRSRHGGMDFAAPEGTPVVAANAGRVIDTGEYFFQGRSIVLDHG
                     QGLLTLYAHLSAIDAPLAAVVEAGSPIGKVGATGRVTGPHLHFSVYLNAVSVDPELFL
                     AAA"
     gene            7625..8059
                     /locus_tag="VIG70_03150"
     CDS             7625..8059
                     /locus_tag="VIG70_03150"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEY8249576.1"
                     /translation="MDRRIAAVFLFVPALLAGCAALAPQSGADSLGGTSWQLVKFQGG
                     DDTVLRPDDKSKYTLAFGADGTVSARIDCNRARGAWKSPEKGRLEFGPMAVTRAMCPP
                     GSLHDQIVKQLPHVRSYVIKDGHLFLSLMADGGIYELEPAAR"
     gene            8056..8301
                     /locus_tag="VIG70_03155"
     CDS             8056..8301
                     /locus_tag="VIG70_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY8249577.1"
                     /translation="MKLWIGNIAPETADDEIRALVKKYASDLECTSIQRVDGDGSRPA
                     AVLEFAAPPLGALQRVSLRLNGMYWKDRALMVQTIIR"
     gene            8298..9095
                     /locus_tag="VIG70_03160"
     CDS             8298..9095
                     /locus_tag="VIG70_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8249578.1"
                     /translation="MTRQDLAKALERVIAHYQPIVALGTGAVAGFEMLARIEDDAGPA
                     RSIGPIIEEVESDPGHVEKLMWRLLQAIQRDVQPLFARFPDFYVSVNVPPAMIGDGRI
                     LAMVKDLGLTPCVNRLVCEVTERQALSAAGRAALDGARRSNVRIAMDDFGTGNSGLAQ
                     LLGMTIDVLKLDRSQVEHLLKDPKADRLVRGIVALANVLRARTVAEGVETAAQAFFLH
                     AAGVDYGQGWFWSKAVPPEELPRVIEQGFGDRQRELLALLHAGAKER"
     gene            complement(9110..9943)
                     /locus_tag="VIG70_03165"
     CDS             complement(9110..9943)
                     /locus_tag="VIG70_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249579.1"
                     /translation="MPMLRALLCVALVSGTAQAQDAGTLRARHAALREQLASNPFGRP
                     LHVESDASGGAHKGEIFAVIDQPYGVVGPALARPANWCDILMLQINIKRCGAADADTL
                     AAFITRKPRDPLDSAHRVDFRYEPAMADADYLRVALGARAGPMGTRDYKILLEAAPLD
                     SRRTFVHMSYAYTLGFAARFAMDAYLAGAGRDKRGFTVDGGERGVVERSAMRHYLAIE
                     AYLASLSAPPGQRLQARLRDWYAATARYPQLREPVGAEEYVEMKVREASPASTGASAP
                     R"
     gene            complement(9974..10930)
                     /locus_tag="VIG70_03170"
     CDS             complement(9974..10930)
                     /locus_tag="VIG70_03170"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEY8249580.1"
                     /translation="MHPYAALTPEVVLDAIDALGLRTDGRLLALSSYENRVYQVWLED
                     DASVVAKFYRPGRWSEAQIDEEHEFARELAEREIPVVAPIHKGKFSDFIYAVYPRRGG
                     RTPELGDPKALEWIGRFLGRIHAVGSTKKFIHREALTAQTFGHDARAYLLKSSLVPAE
                     LLDAWKAVTEQALRLVDSCYQRCEARAIRLHGDCHPGNILWTHEGPHFVDLDDARMGP
                     AMQDLWMLLSGDRAAMSLQLHHVLTGYEQFMRFDRSELHLLEALRTLRLIHYSAWIAR
                     RWDDPAFPAAFPWFNTQRYWQDRILELREQIALMQEEPLAVD"
     gene            10948..>11682
                     /locus_tag="VIG70_03175"
     CDS             10948..>11682
                     /locus_tag="VIG70_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249581.1"
                     /translation="MQQRRITQAAVEIRNLHFSYGDLKVLRGLSLNIPRGEVVAILGG
                     SGSGKSTLLKLIGGQLRPEKGTVRVEGKNVHELSSDELYQLRLQMGMMFQTSGLFSDL
                     SVYENVAFPIRENFVDLPEALVRRLVLMKLHAVGLRGAQAMMPNDLSGGMTRRVALAR
                     AIATDPKLMMYDEPFAGLDPISLNQVGVLVRELNQSLGLTSIVVTYDVQEALKVAGYV
                     YVINDGSVLGKGTPQEIVSSEDPYLKQ"
BASE COUNT         1941 a         3842 c         4026 g         1873 t
ORIGIN      
        1 cgcccacggc cggctctatt tcgaggagcg cggctggggc gagcgcttcg aggccctggt
       61 cgccggcatc gcgaaggact tcatcgaaaa attcgatccg tcccgcgagc gctgctggat
      121 cgccgagatg gacggcgagc cggtcggctg cgtgttcgcg gtgaaggaat cgaaaaccgt
      181 ggccaagctc cggctcctgc tggtggagcc caaggcccgc ggccgcgggc tcggcaggcg
      241 cctggtggaa gagtgcatcg ccttcgcccg cgccaagggc taccgcaagc tcgtgctatg
      301 gacgcagtcc aacctcaccg cggcgcgggc gatctaccgg aagaccggct tcacgaaagt
      361 caaagaggaa aagcacgcga gcttcggggt gaggctgaac ggggagtact gggaactcac
      421 gctctaggaa ttcggggacg gagccctaac tccgaaagct gcgccagccg tggagttagg
      481 gctccgtccc cgaattccca aagctctaga cgggtttgta gcggatgacc ttgttgcgcc
      541 cttcctgttt cgcctggtac agagcgcggt cggcgcggga cgccagcgcg tcgagggtgc
      601 ggccgtcctc cgggtagcac gcgatgccga tgctcaccgt ggcggtgatg cgctggccgt
      661 cgaaggcgat cggccgcgcg ccgatcgcgt tgcggatgcg ctcggcgacg tcggcggcgc
      721 ctttgggcgg cgtctccggg aggagcacga tgaactcgtc gccgccgtag cgcgccggca
      781 cgtcggtcgc gcgcagctcg gactgcaccg cgttcgccac ctggcgcagg agccggttgc
      841 cggcctcgtg gccatggctg tcgttcaccg gcttcaggtt gtcgcagtcc accatcagca
      901 ggctggcggg gcggccgtag cgcaccgcct ggccgaacag gcggttggcg gcgatcgcga
      961 agccgcgcgt gttgtagagc ccggttagct cgtcggtctc ggagaggagc ttcgcctgct
     1021 gcaggccgta gcggatgtcg gccgagaaca tggtggtgat gtaggcgacg aggagcaccg
     1081 gcgcgagctg cccggcgaag cccgcgatga agcgcaaggt gagcacttcg tccatggtcg
     1141 cgctgccgag caggatgtag cacgaggcca ccagcccgac attggcgagc gtggcgagct
     1201 tgcccagggt cagcgacgag gtgatcaccg gcagcaggta gaggttgacc agcgggctcg
     1261 ccaggcgctc ggtgtaccac agcacccagg tgatgaaggc gatcatgccg agcgtctcga
     1321 tcgcgatctt ccagcgcgtc tcgcgcttgt agaagttcgc atagcgaaag ctcatgacca
     1381 tcgcggcgta gaagaaaagg cccgccgaca ccgcggcgtc ggcctcggga tcgccgcgct
     1441 cgccgccgaa cacgagatac agcagcacga ggatcagcag cagccagtgg atctcggcga
     1501 cggtgcgcga gatgcccctg agctcttcct gctcgatcga gatcgagacc ctttgttctt
     1561 gttcggccac tgcgactccc gtagaccccc gtgtattatg cggcggcatg gaaatctctg
     1621 ccaaaggctt gcgcgtcctg gtgaccgcgg gcgcggccgg catcgggcgc gtgataacgc
     1681 gcacgttcgt cgagcacggc gcgcgcgtgc acatctgcga cgtcgacgaa aaagcgctcg
     1741 ccgagataaa aagggagctt ccctcggtga gccagacggc cgcggacgtc gcgcggatcg
     1801 acgacgtcga gcgcctgttc gaggacgtga aaaaagtcct gcgcggcctc gacgtgctgg
     1861 tcaacaacgc cgggatcgcc ggacccacgg caaaaatcga ggacatccgc cccgaggact
     1921 gggaccgctg catcgcggtg gacctcaacg gcatgttcta ttgcacgcgc aaggcgatgc
     1981 cgctcatcaa ggccgccggc ggcggcagca ttatcaattt gtcttccgcc gccggaaggc
     2041 tcgcgttccc gatgcgcacc ccttactccg ccgccaagtg ggcgatcgtc ggcttcaccg
     2101 agagcctcgc cgccgaggcc ggcccggaca aggtgcgcgt gaactgcatc cagcccggca
     2161 tcgtcgaagg cgagcgcatc gaccgcgtga tcgcggccaa ggcgaaggcg ctcggcgtcc
     2221 cggaagggga agtgaaatcg aagatggtcg agggcgtgag catgaagacg acggtcaccc
     2281 agcaggacat cgccgacatg gcgctctacc tcgccaccgc cgggcggcac atctcgggcc
     2341 aggcgatctc ggtctgcggc ggcacccgct acctcgtcta ggaattcggg gacggagccc
     2401 gaactggtta gggctccgtc cccgaactag aatcggatca tggcagcggc ccaaggcaaa
     2461 gtcatcatca cctgcgcagt gacgggctcg atccacacgc cgagcatgtc gccgcacctg
     2521 ccggtgacgc cggcggaggt cgcccggggc gccatcggcg cggcgcaggc gggcgcggcg
     2581 atcgtgcacc tgcacgcgcg caacccgaag gacggcagcc cctcgcagga cccggcgctg
     2641 ttccacgagt tcctgccgca gatcgcggtc gcagccgacg tggtcatcaa cctcaccacc
     2701 ggcggcgcgg cgaccatgac gaccgaggag cggctgcagc ccgcgctgca gctcaagccc
     2761 gaggtcgcct cgctcaacat gggctcgatg aacttcggcc tttacgagat gctagggcgc
     2821 tacaaggaat tcaagtacga ctgggagaag ccctacctcg cgggctccga cgatcgcatt
     2881 ttcaagaaca ccttcaagga catcgcctac atcctgcagt cgtgcagcga gaacggcacg
     2941 cgcttcgaga tcgagtgcta tgacatcggg catctgtaca ccgcggcgca cttcgtggat
     3001 cgcgggctga tcaagccgcc gatgctgatt caatccgtgt tcggcatccg cggcggcatc
     3061 gggccgcacc ccgaggacgt gatgcacatg aagcgcaccg ccgatcgcct gttcggcgac
     3121 gcgtactact ggtcggtgct cggcgccggg cgcaaccaga tgttcatcgc cgcgatgtcg
     3181 gcggtgatgg gcggcaacgt ccgggtgggg ctggaggaca gcctgtggct cggccgcggc
     3241 cagctcgcca aatcgaacgc cgagcaggtg gcgaaggcga agcgcatgct ggaggagctg
     3301 gggctgagcg tagcgactgc gaacgacgcg cgcgagatgc tgaaactcaa aggaggacgc
     3361 aatgtcgctt tttaggtttg ttcttctgct ggctttttcg ctgccggtcc tggcgcagga
     3421 ctggccgaac aagccggtca agttcgtctc gccgtacccg ccggggggct cggtcgatcc
     3481 gctcgcgcgc atgctcggcg cgaagctcgg cgagtcgctg aagcagaact tcatcgtcga
     3541 gagccgcacc ggcgcctctg gagtcatcgg caccgactac gtcgcgaaga gcgcgcccga
     3601 cggctacacc ttcgtcttca tcttcgacac ccacgcggtg caccaggcgc tcaatcccaa
     3661 gctacccttc gacccgctga aggatttcgc cccggtgatg atcgtcggca ccgcgcccat
     3721 ggcgatcacc accggcacgc aaaagccgta caggaacttc caggatgtgc tgaaggtggc
     3781 aaagtcgggg ggcgtatcgc tcggcaacgt cggcaacggc tcgctcgccc acctcacgac
     3841 catcgtgctc aaccaggccg ccgggaccaa gttcgagccg gtcgcctaca agggcggggg
     3901 accgctctcg acggacttga tcggcggcca cgtggagctc gcaatggcgt ccaccgccgc
     3961 gcaagcgcag cacgtgagaa gcggcaagat gcgcgcgctg gtgcttaccg gcgacaagcg
     4021 ctcgcacacc atgcccgacg tacccaccct gaaggagctc ggcatcgacg tggtggcgca
     4081 cgcctggtgg ggcatcctcg cgcccgcagg cacgcccaag ccgatcatcg acaagatcca
     4141 cgccgagctg gtcaaggcga tcaagctgcc cgacgtcaac aaaatgctca ccgagaccca
     4201 gggcatggac gtggtcgcgc ttagccccga ggccacgcgg aagtggatcg cggacaacat
     4261 ggcgcgctgg ggcaaagtgg tgaaggacaa cgacatcaga gcggaatgaa gctggcggtc
     4321 ctcgacgact accagcgcct cgcgctggag tacgccgact ggaagtccct caaagacgtc
     4381 gaggtcgcgg tctttgatga acccctgaag gatgccgcgc agcagctcgc gcccttcgac
     4441 atcgtctgcc tgatgcgcga gcgcacgccg ttcccgcgcg cgctgatcga aaagctgccc
     4501 aacctcaagt tcgtcagcct caccggcgcg cgctcgccga gcctcgacac gcaggcgctg
     4561 aaggagcgcg gcatcccggt gagcaacacg cgctccgccg gcggcgggct ctcgacggtg
     4621 gagctgacct ggggattgat catcgccgcg gcgcgcggcc tcgcgaaggg cgagcgcaac
     4681 atgcgagcgg ggcggtggca tgagggtctt tctcccggat tttcattggc gggaaagcgc
     4741 ctcgggctcc ttggtctcgg caacatcggc gcacgcgtgg ccgcgatcgg aagcgcgttc
     4801 ggcatggaat gcgccgcgtg gagcgcgaac ttgacggcgg agaaagccgc ggccgctggg
     4861 gcttcgctcg tctcgaaaga aaggctgttt gccgagtcga acgtcgtgac gatccacctc
     4921 gtcctcggcg atcgcacgcg cggtctggtg ggtgccgccg acctggcgcg catgaagccc
     4981 ggcgcagtgc tcgtgaacac ctcgcgcggg ccgatcgtgg tcgaaaacga cctcctcgct
     5041 gcattgaaag caggaaaaat ccaggctgcc ctggacgtct acgagcacga gccgctaccg
     5101 gccggccatc cgctgcgcaa gatggagaac gtgaccctct cgccccatct tggctacgtg
     5161 cacgaggaca actaccgcat cttctaccgc gacacggcgg agaacgtcgc cgcctggctc
     5221 gccggcaagc cgatccgcgt catcaacccg tgaggctttc gtacctcccg ccgctgatgg
     5281 tgtacgcggc gtacggggtg tcggggctga cggggatcgt cggcacgttc ttcgtcaagg
     5341 actacctcga gctctcgccg gagttcctcg ccgcgctgct gttctgggcc ggaattccct
     5401 gggcgctgaa aatgccggtc ggccacgtgg tggacctcat ctggcgctgg aaggcggcgc
     5461 tggtgtggct cggcgcctcg atgatcgcgg cgagcctgct gatcatgatc ggcctcatca
     5521 gccacaccga agcgatgcgc tcggtgatga gcaccgggag ctggttcgtg ctctcgtcgc
     5581 tgctcgcgcc gaccggctac atgatccagg actcggtcgc cgacgccatg acggtggagg
     5641 ccgtgccgcg ggtcgatgac ccgaagttgc aaaagagcat gcatacgacg atgcagacgc
     5701 tcgggcgcgt tgcgctgatc ggcggcctga ctctggtagc cgcagcgaac gtctacatct
     5761 tccatgacgc cgaagccctg ccgaaagagc agaaggccgc gctctatgcc gacgtctaca
     5821 ccgccgcgct cgtcgtcccg gtgctgtcgg tgctcggcgt gatcctgcac cgcttcctca
     5881 agcacgagcg cgaggtggtc gagaagaccc ggccgaactg ggcggtgctc gggggcagcc
     5941 tggcgttcgt cgccgtgacg ctcgccgtcg gcgtgggcgg ggtcgcgaac gccgaggaga
     6001 tcatcttcgc cgggtcgctc gcggtgatcc tgttcctgct gtggcgcctc gcgcgcgagc
     6061 tcgagcccgc ggcgcgcaac gtgctggtcg cgaccgcggt ggtgctgttc ctctaccgcg
     6121 cggtgccgct caccggcgac ggctcgacct ggtggatgat cgacgtgctg aagttcaacg
     6181 aggcgttcct cgcgcagctt tcgctcgtca cctatgcgct cacgctggtg ggaatgttcg
     6241 cgctgcgccg cttcatggcc gagcactcga tcgccaccat cgtggtggtg ctgagcgtgg
     6301 ccgcggcggc gctcgcgctc cccaccatcg gcatgttcta tggcctgcac gagtggaccg
     6361 cggcgcacac cggcggcagg gtcgatgcgc gcataatcat gctggtgaac acggcgctcg
     6421 agtcgccgct cggccagatc gccatgatcc cgatgctcgc ctggatcgcc aactcggcgc
     6481 cggcgcacct caaggcgacc tttttcgcgg tgatggcttc gttctccaac ctggcgctcg
     6541 ccgcctcgca gctcggcacg gtatacctca accgggtatt cgtggtgaag cggggggact
     6601 acagcgagct tggtatcctc atggtcgtcg ccgcggtgct cacgctggcg ctgccgctga
     6661 tcgcggtcgc cggtgtgcgc ctgctgcggc tgcgcaccgc ttgacggatc gcccggcgaa
     6721 ggaacctccg tgcaccccgg tggccaaagc aggtgaaaaa ggaggctcga tgaagcggcg
     6781 caggatcctc cagtcgctcg ccgcggtgct cgttgcgcag gcatggggcg tccctgcatg
     6841 ggcttcggcc tacccgcgaa ccgcggcagt cccgggcggg gtcgccagga tccggctcgg
     6901 cgctgccgag cgcgcgccgc gcgtgctcct cgaaggcgag cgcgtgctgg tcgtgcgcga
     6961 agacggcgaa tgggtcgcgc tggtcggcat cgcgctgacc gcgcggcctg gctcgaaggt
     7021 gcggctcgag gtcgagcacg ccgacggcaa gcgcaagcag ctcgaggtca aggtggctgc
     7081 gaaggcctat gcgtcgcagc acctcaaggt gccgccgggc caggtggagc tttccgcgga
     7141 ggatctggcg cgccacgagc gcgagcggac gcatctgcgc gaggtgctgc gcacgttcac
     7201 cgattccgcg cccgccactc tcgcgatgct ccagcccgct cccggccggc gcagcagcgt
     7261 cttcggcctg cagcgctact tcaacggcca gctgcgcagc cgccacggtg gaatggattt
     7321 cgcggcgccc gagggcacgc cggtcgtcgc cgcgaacgcg ggccgggtga tcgacaccgg
     7381 cgagtacttc ttccagggac gcagcatcgt cctcgaccac ggacagggat tgctcacgct
     7441 ctatgcccac ttgagcgcca tcgacgcgcc gctcgcagcc gtcgtggagg ccggcagccc
     7501 gatcggcaag gtgggcgcca ccggccgggt caccgggccg cacctgcatt tctccgtgta
     7561 tctcaacgcg gtctcggtcg atccggaact ttttctggcc gcagcctagg agtaccctcg
     7621 ggtagtggat cgacgcatcg ctgcggtctt cctgttcgta ccagcgctgc tggccgggtg
     7681 cgccgctctc gcgccgcaga gcggtgccga cagcctcggc ggaacctcct ggcagctggt
     7741 gaagttccag ggtggggacg atacggtgct gcggcccgac gacaagtcga agtacaccct
     7801 tgcgttcggc gccgacggca ccgtcagcgc gcggatcgac tgcaaccgcg cccgcggcgc
     7861 ctggaagtcg cccgagaagg gacggctcga gttcggtccg atggcggtca cgcgcgcgat
     7921 gtgtccgccc ggctcgctgc atgaccagat cgtcaagcag ctgccgcacg tccgctcgta
     7981 cgtcattaag gacggccatc ttttcctttc gctgatggcg gacggcggga tctacgagct
     8041 cgagcccgcg gcaagatgaa gctctggatc ggcaacatcg cacccgagac tgcggacgac
     8101 gaaatccgcg cgctggtgaa gaagtacgcg tcggacctcg agtgcaccag catccagcgg
     8161 gtcgacggcg acggctcgcg cccggcggcg gtgctcgagt tcgcggcgcc gcctctgggt
     8221 gcgctgcagc gcgtctcgct gcggctgaac gggatgtact ggaaagatcg cgccctgatg
     8281 gttcagacga tcatccggtg accaggcaag atctcgcgaa ggcgctcgag cgcgtcatcg
     8341 cgcactacca gccgatcgtc gcgctcggca cgggcgccgt cgccgggttc gagatgctgg
     8401 cgagaatcga ggacgacgcc ggcccggcgc gctcgatcgg gccgatcatc gaggaggtcg
     8461 aaagcgaccc ggggcacgtg gagaagctga tgtggcggct gctgcaggcg atccagcgcg
     8521 acgtgcagcc gctgttcgcg cgctttcccg atttctacgt gagcgtcaac gtgccgccgg
     8581 cgatgatcgg cgacggcagg atcctcgcga tggtgaagga cctcgggctc acgccctgcg
     8641 tcaaccggct ggtttgcgag gtgaccgagc ggcaggcgct gagcgcggcc gggcgcgcgg
     8701 cgctcgacgg cgcgcgcagg tccaacgtgc gcatcgcgat ggatgacttc ggcaccggca
     8761 acagcggcct cgcacagctc ctcgggatga cgatcgacgt gctcaagctc gatcgctcgc
     8821 aggtcgagca tttgctgaag gacccgaagg ccgaccggct ggtgcgcggg atcgtcgcgc
     8881 tggcgaacgt gctccgggcg cgcaccgttg ccgaaggcgt tgagaccgcc gcgcaggcgt
     8941 tcttcctgca cgccgccggc gtcgactacg gccagggctg gttctggagc aaggccgtgc
     9001 cccccgaaga gctgccgcgg gtgatcgagc aaggcttcgg agaccggcaa cgcgagctgc
     9061 ttgcgttgct gcacgcgggg gcgaaggagc gctgagcagt cgaaatgggc tagcgcgggg
     9121 cgctggcgcc tgtcgacgca ggactcgctt ccctgacctt catctcgacg tattcttccg
     9181 cgccgaccgg ctcgcgcaat tgcgggtaac gcgcggtcgc cgcgtaccag tcgcgcagcc
     9241 gtgcctgtag gcgctgcccc ggcggcgcag agagcgacgc gaggtaagcc tcgatcgcga
     9301 ggtaatggcg cattgcgctg cgctcgacca cgccgcgctc gccgccgtcg acggtgaagc
     9361 cgcgcttgtc gcgtccggcg ccggcgaggt acgcgtccat ggcaaagcgc gccgcaaagc
     9421 cgagggtata ggcgtaggac atgtggacga aagtgcgccg ggaatcgagc ggcgcggcct
     9481 cgagcaggat cttgtagtcc cgcgtgccca tcggcccggc gcgggcgccg agcgcgaccc
     9541 ggaggtagtc ggcgtcggcc atggcaggct cgtagcggaa gtcgacgcgg tgcgcgctgt
     9601 cgagcgggtc gcggggcttg cgggtgatga atgcggccag cgtgtcggca tcggcggcgc
     9661 cgcagcgctt gatgttgatc tgcagcatca ggatgtcgca ccagttcgcc ggacgcgcga
     9721 gcgccggacc gacgacgccg tagggttggt cgatgacggc gaagatctcc cccttgtgcg
     9781 cgccgccgga agcgtccgac tccacgtgca gcgggcggcc gaacgggttg ctcgcgagct
     9841 gctcacgcag cgctgcgtgg cgcgcacgca gcgttccggc gtcctgcgcc tgcgcggtgc
     9901 ccgataccaa ggcgacgcac aacagggcgc ggagcatggg catggcgagt gcccagcaag
     9961 aaacggtcct tctctagtcg accgcgagcg gctcctcctg catcagcgcg atctgctcgc
    10021 gcagctcgag tatccggtcc tgccagtagc gctgcgtgtt gaaccacggg aacgccgccg
    10081 ggaacgccgg gtcgtcccag cgccgcgcga tccacgccga gtagtggatg aggcgcaggg
    10141 tgcgcagcgc ttcgagaaga tgcagctcgc tgcgatcgaa gcgcatgaac tgctcgtagc
    10201 ccgtcagcac gtgatgcagc tgcagcgaca tggccgcgcg gtcgccggag agcagcatcc
    10261 acaggtcctg catcgccggc cccatgcgcg cgtcgtcgag gtccacgaaa tgcgggcctt
    10321 catgggtcca caggatattg ccgggatggc agtcgccgtg gaggcggatg gcgcgcgctt
    10381 cgcatcgctg gtagcaggaa tcgaccagcc gcaacgcctg ctcggtgacc gccttccagg
    10441 catcgagcag ctccgccggc accagcgagc tcttcagcag ataggcgcga gcgtcgtggc
    10501 cgaaggtttg cgcggtcagc gcttcgcggt ggatgaattt cttcgtcgag cccacggcgt
    10561 gaatccgccc caggaagcgg ccgatccact ccagcgcctt cgggtcgccg agctccgggg
    10621 tgcgcccgcc gcggcgcgga tagacggcat agatgaagtc ggagaatttt cctttgtgta
    10681 tcggtgcgac gacaggaatc tcgcgctcag ccaattcccg ggcgaattcg tgctcctcgt
    10741 cgatctgcgc ctcgctccag cgccccgggc ggtagaactt cgccaccacc gaggcgtcgt
    10801 cctccagcca cacctggtac acgcggttct cgtagctgga cagcgcgagc agtcggccgt
    10861 cggtgcgcag gccgagcgcg tcgattgcgt cgagcaccac ctccggcgtg agtgcggcgt
    10921 acggatgcat tggcgtatag tagttctatg cagcagaggc gcatcacgca ggctgcggtc
    10981 gagatccgca acctgcattt ttcgtacggc gatttgaagg tgctgcgcgg cctttcgctc
    11041 aacatcccgc gcggcgaggt ggtcgcgatc ctcggcggca gcggctccgg aaaatcgacg
    11101 ctgctcaagc tgatcggcgg ccagctgcgc cccgagaagg gcacggtcag ggtcgagggc
    11161 aagaacgtcc acgagctgag ctccgacgag ctctaccagc tgcgcctgca gatgggcatg
    11221 atgttccaga cctcgggcct gttcagcgac ctgtcggtgt acgagaacgt ggcgttcccg
    11281 atccgcgaga attttgtcga tttgcctgag gcgctggtta gacgcctggt tttgatgaag
    11341 ctgcacgcgg tcggcctgcg cggcgcccag gcgatgatgc cgaacgacct gtcgggcggc
    11401 atgacgcgcc gcgtggcgct cgctcgcgcc atcgctaccg accccaagct gatgatgtac
    11461 gacgagccct tcgccggact cgacccgatt tcgctcaacc aggtgggcgt gctggtccgc
    11521 gagctcaacc agtcgctcgg cctgacctcc atcgtggtga cctacgacgt gcaggaagcg
    11581 ctcaaggtgg ccggctacgt ctacgtcatc aacgacggca gcgtgctcgg caaggggacg
    11641 ccgcaggaga tcgtctcctc cgaggatccc tacctcaagc ag
//
LOCUS       DATBSZ010000049        18344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_894307, whole genome shotgun sequence.
ACCESSION   DATBSZ010000049 DATBSZ010000000
VERSION     DATBSZ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18344
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894307"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VIG70_03180"
     CDS             complement(<1..948)
                     /locus_tag="VIG70_03180"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8249582.1"
                     /translation="MKIIALEEHYWDPEVATHFKERGPEMRIPALQERLFDLGALRIK
                     EMDEAGIDLQVLSHGAPATQRLDPETAVPLARRANDRLHEAVSKHPGRFEGFAVLPSA
                     DPKAAADELERAVTRLGFKGAMIHGLTNNLFIDDKRFWPIFERAQALDVPLYLHPAVP
                     HKAVIDVYYRDYLEQFPGLMTAAWGFGVETATQGIRLVLSGVFEAYPGLKIILGHLGE
                     GLPFYLWRIDMALARQGNRKTPFRDTFREHFWITTSGNFSTPSLLCCVMEMGADRILF
                     SVDYPFVPNPPGVKWMEHVPLGPEDRAKILHGNAQRLLKL"
     gene            complement(945..2222)
                     /locus_tag="VIG70_03185"
     CDS             complement(945..2222)
                     /locus_tag="VIG70_03185"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY8249583.1"
                     /translation="MSFALGVLPVALLLLGFPIYLVLLTAVSVALIFYMNVPLVVLHQ
                     NLFGAIDAFALLAVPFFIYAGELMSRGSVAQRIVDFVQAGVGRVPGSLGVTTVGTSAI
                     FGAISGASAATVATVGKAMLPAMRRAGYPEPFAAGLITAVGAIDIIIPPSIPLIVYGM
                     AAQESVPRLYAAGVLPGLLIAGLLAIYVVWTARRQGFEVGSPFQLSAFLGAAARGAWA
                     LGAPFIILGGIYGGIFSPTEAAAVACVYAALVTWVVFRELGWSDVVEAAASTALFTGQ
                     VLIIVACAGVFAWLLTVQQVPQALVAWIQSLGVSQGEFLLVANVLLLLVGCFLDPLSA
                     ILLLTPLLIPVVKALGIDTVHFGIVVTVNLAIGLFHPPFGINIFVAQSALGLKLEVIY
                     RGIVPFIVIYLGALALITYVPQISLAGVRWFLL"
     gene            complement(2219..2659)
                     /locus_tag="VIG70_03190"
     CDS             complement(2219..2659)
                     /locus_tag="VIG70_03190"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY8249584.1"
                     /translation="MNRLMTAIERILALAFIAAVCLNFANVVGRYGFGRSIAAADEIQ
                     IYIMVFMAFLGAAVVSWRRQHLRMDVLVRFLPPGLQSALRICEIVLVAAVAGFVLVQS
                     WRYSAQMLALGRSSDVGGIPMWIPHGAVTLGFFLILIVSLRNSK"
     gene            2689..3654
                     /locus_tag="VIG70_03195"
     CDS             2689..3654
                     /locus_tag="VIG70_03195"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249585.1"
                     /translation="MLVKLFAGLFAGAIAFAAPLAGAQQYPSKPVRVIVPFAPGGGSD
                     FTARLMAQKLTERLGNPFVVENRAGAGGNLGAEMAVRSPADGYTLLLISASYTVNPSV
                     YKLSFDSVQDIAPIIQISGGPYVVAVHPSVPAKTLAEFVAYARQQPEKLAYGSSGNGS
                     VMHVSTAYFLESARIKVLHIPYKGTGPALQDTIGGQVQLVFGAVPTTLPHVRAGKLRA
                     LAVTTAKRIAAAPEIPTIAESGYPDYEVTNWHGLVAPKGAPREVVERLNKEINEMLTS
                     EDFRKVLANDGLEPAGGSPARFGEVLAKETARWKAVVQQAGIKVE"
     gene            3651..4421
                     /locus_tag="VIG70_03200"
     CDS             3651..4421
                     /locus_tag="VIG70_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8249586.1"
                     /translation="MKTNHRIDGRDGRGGRWVTFVTGIANDLTMWDGQVPALAGDFRI
                     LRYDLRGHGGSEATGGDYSMALLVEDLKALLDAQKIQKTSLVGLGLGGALAQAFAIEH
                     PDRLDRLMPCCCRARMVPDFAAMWHKLRETVRQNGLETIVEPTVQRWFSEDFKAAHPD
                     VLDNIRRMIRGTTLEGYLGVTAAFLGLDVEDRLQEIRAPTLYVSGAEDKLGGPPALMQ
                     GLSGKVKGARHLSVPKAAHIANIQNPEGFNRVLGEFLK"
     gene            4418..5359
                     /locus_tag="VIG70_03205"
     CDS             4418..5359
                     /locus_tag="VIG70_03205"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249587.1"
                     /translation="MRLFLVLLFFCSAAGAQPYPSKPLRWIVPTGPGSAVDVNARRVT
                     PKLAEALGQPVIVENRPGANSMIAAREAARAAPDGYTLFQAVVNNAINDALAPDPCCV
                     LNDKLVPVTRLYTTPLVMVVHPSLPGNLKDYVALAKSKPNSLTYAHGGTGAITQMVGE
                     LLKLTAGIQVREVPYKAIGAELPDLTGGHVSTAFLSPVVIRDMVRAGKLRALAIADRQ
                     RLDILPEVPTMAEGGYPIEGLGWNGIFVPAGTPRPVVDRLHKEITRVLQLKEIQDDAM
                     ALGTRAGGEKPEEFAAFVRSEVQKWGKVIKDAAIKAQ"
     gene            5380..6336
                     /locus_tag="VIG70_03210"
     CDS             5380..6336
                     /locus_tag="VIG70_03210"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249588.1"
                     /translation="MKLFSLIAGLFLTAACYAQAWPNKPVRVIVNVAAGGVADVTMRV
                     LGARMTETLGQSFLVENRAGGDGYIGFDAVARSDPDGHTLLYSPGSSMMIAPHIVNRP
                     DLDPLKVLVPIAASGRVSLYVIAHPERPFKNLNELLAYARANPGKLNYGTPGNGTSPH
                     IATEVFNREAKVKLNHVPYKGAGPALQDLIGGQIDIVFDPGVGIAPAKAGKVRMIAIA
                     GPKRHPDFPDIPTLDENGIKGVDGGPHFGLYGPAGTPRDVVLRINSELLKAMQHPAVA
                     ERFKALAVDVAEPMSPEQFAAYVRGEHARYAKLIPELGIKTQ"
     gene            complement(6323..7177)
                     /locus_tag="VIG70_03215"
     CDS             complement(6323..7177)
                     /locus_tag="VIG70_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8249589.1"
                     /translation="MKLRSVELELPDVAQAADFLGSVWGLAPAGTSGATQFFRGTGNH
                     PYILSITRASAPAVAAITFSGAPDELHSVRQRNGSPRMIKFNVPGGGEGFEVRGPEAQ
                     TYRFIEETGVPERLKDADKPIQLTHAVVNSTDVEAAERFAVEKLGFQVSDRTAHMRFL
                     RCNRKHHCVAYAKSELASLNHIAFEMPSLDAVMRGMARVREAGFEPVWGPGRHGPGNN
                     VFGYFIAPYGGVIEYTAEVSEVGDDYKVGGPEDWKWPPGRIDHWGLSKKDTAKTGPAE
                     RVFRFTGS"
     gene            complement(7174..8535)
                     /locus_tag="VIG70_03220"
     CDS             complement(7174..8535)
                     /locus_tag="VIG70_03220"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HEY8249590.1"
                     /translation="MNDSFDVAVVGLGPTGAVCTALLGGLGLKTIAIEREQAVYDKPR
                     AFALDHEIMRVFEDIGIAEAVAPHTALFTSSEYYGVDGQLIKRLGALPPPWPLGWPPS
                     MVFNQPAVDALLRDRAAAVAEVAFTELEGLDPSKKDVKLRLRDRTITAKYVVACDGAS
                     STVRGALGIEYEDLDFDQPWLVVDLLVSPRGLEKLPKVSIQYCEPARPSTYLIGVGAH
                     RRWELMLDPGEEPDVWKLLARWITPDDAQLWRAASYRFHALVAREWRRGRVFLAGDAA
                     HQQPPFTGQGMCQGIRDAANLSWKLHYVLAGKAKERLLDTYEAERSAHAKRLISIVKD
                     IGAVIGERNVEKARARDRWHLAETGGKVVTVPRQHLIPPLKDGFLSSRPHAANGTLFP
                     KDGGTGMRTVKRDPSQTLVADWFDKNICTAAIVRPDHYVYGVARDDAALDRMLKEMAK
                     GLK"
     gene            8546..9376
                     /locus_tag="VIG70_03225"
     CDS             8546..9376
                     /locus_tag="VIG70_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8249591.1"
                     /translation="MPFKPPPLACDAHCHVFGPAAKFPYDPKAAYIPADAPFEALQKL
                     HGRLGIERAVIVHASCHGADMRVTLDAIARSGGRYRGTAIIDESYGDPEFRKMHDGGI
                     RGVRFNFVRHLGGRPEMGFFTRTVERVQAMGWHLILHLDAADLVEFDALFRKIRAPMV
                     IDHMGRVKAADGLQQKPFQVLLEWMKQDNFWVKICGAERVSSKGPPFTDAVPFGRALV
                     EAAPERVLWGTDWPHPNVKWMPDDGALVELFPLMVPEPALQQRILVDNPESLYGFQGR
                     "
     gene            9379..9900
                     /locus_tag="VIG70_03230"
     CDS             9379..9900
                     /locus_tag="VIG70_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249592.1"
                     /translation="MLGLTTLGAIHTTISLIALAAGFVALARHWEISNASRSGKVFVW
                     GTVLSCLTGLGIFQHGGFGNPHILAIVTLVVMAVALAAQYTAVFGRLSRYVSTIGYSL
                     TLFFHFIPGTVETLTRLPVGAPYLSGPLDPKAQPILGFFFLLFLIGATLQVVRLRAGR
                     ETEPLLADRQEES"
     gene            complement(9902..10501)
                     /locus_tag="VIG70_03235"
     CDS             complement(9902..10501)
                     /locus_tag="VIG70_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY8249593.1"
                     /translation="MLWEMTMRNAALALLAFCLGWIPLAGAADLDRPLILVAKPELQD
                     KLYGSSVLVVAPVGGGQHAGFIVNRPTRVTLGSLFPEHGPSQKIVDPVYLGGPIQSQV
                     VFALVQRRDNPGGSSFEMMPGLFAAFDAEVVDRIIESDPTRARFVAGLVAWRAGELKD
                     EIKQGAWYVLEPDAALFMRNPDGLWEELVRRSVRLGNTI"
     gene            complement(10574..11356)
                     /locus_tag="VIG70_03240"
     CDS             complement(10574..11356)
                     /locus_tag="VIG70_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEY8249594.1"
                     /translation="MQLQGKSALVTGAASGIGKEIALAYAREGAKVAIGDLDKRAADA
                     AAAEIKATGGQAIGVAMDVANEDAVNAGVAAMVAAFGGVDILVSNAGIQIVHPLEQFP
                     YAEWKKMLAIHLDGAFLTTRACLQHMYKSGRGGAVIYMGSVHSKEASVLKGPYVTAKH
                     GLIGLAKVVAKEGAKHGVRANVICPGFVRTPLVDKQIPEQAKELGITEAEVIKNVMLK
                     ETVDGEFTTVQDVAEAALFLAAFKTNALTGQSLIVSHGWFME"
     gene            complement(11375..12490)
                     /locus_tag="VIG70_03245"
     CDS             complement(11375..12490)
                     /locus_tag="VIG70_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8249595.1"
                     /translation="MTANWGKYDHAVLVLQGGGALGAYQAGVYEGLAECGAAPDWVAG
                     VSIGAINAALIAGNPPERRVARLREFWDRVSAQLPFIPPAAMDPMRPMLDQMSTASAF
                     VFGVPGFFSPRMVPPFLAPDGSIGALSFYDTQPLKATLEELVDFALINRNGVRLSLGT
                     VDLRTGNSVYFDSKTMPIRAEHVLASGALPPGFPPVEIDGELYWDGGIVSNSPLWYVL
                     DADPEMSALIFQVDVFSGAGEAPRNLAEVQERAKDIQYASKTRFNTSRVKEIEALRGA
                     LRRVLEKLPEALRDDPDVQRLAAASKPRACALAHFINRHHPRATSFKDCEFSRATVTD
                     LWQGGLGDVRRSVAGPQWHRATDLAEGLRVFDMTPRT"
     gene            12576..13406
                     /locus_tag="VIG70_03250"
     CDS             12576..13406
                     /locus_tag="VIG70_03250"
                     /EC_number="1.13.11.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gallate dioxygenase"
                     /protein_id="HEY8249596.1"
                     /translation="MARIIGGIGTSHVPTIGIAYDKGKQEDPDWKPLFRGYEPVAKWL
                     AEKKPDVLFFCFNDHATTFFFDHYPTFALGVSDKYRIADEGLGVRNIPPLRGHAALAR
                     HIATSLVNEEFDLTLFQDLPLDHGLHSPLTMMWPKSPDWPGAVIPLVVNVLQHPLPTP
                     RRCYKLGKAIRRAVESFPENLKVAMVGTGGLSHQMIGERAGFNDEAWDREFLDLIERD
                     PERLASMTIEDFMRRGGNEGAEVIMWLIMRGALDERAKKLHQNYYLPMTTAMAVALFE
                     "
     gene            13403..13720
                     /locus_tag="VIG70_03255"
     CDS             13403..13720
                     /locus_tag="VIG70_03255"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="extradiol catechol dioxygenase that catalyzes the
                     oxidative cleavage of substituted catechols; part of the
                     bacterial aromatic compound degradation pathway; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEY8249597.1"
                     /translation="MKYVFDLDRSARGLRLNRFLHGLTSKEARTLFLSKPEEAMQGLT
                     EPEKDMVRRLDWKAMQDYGASFFCLEKLARTKGVSNPEMVAHFRGETLEQFLKTRRVP
                     GAR"
     gene            13720..14541
                     /locus_tag="VIG70_03260"
     CDS             13720..14541
                     /locus_tag="VIG70_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8249598.1"
                     /translation="MKLKTLISDYPVTKEFRTHTERFEFVPFQGGVAQQFQRVVRKLE
                     FDVAEIALMTFLIAKAHGKPYRLLPFTVMARYQHPFLKFNPERTPGLKPGDLAGKRVG
                     VRSYSVTTGAWIRHILAGDYGVDLAKIRWVTFEEAHVAEFVDPPNVERATAGKEIKAM
                     LLAGELDAAILGDSTPDPRLQPLIGDAEAAGRRWSEKHHAIQLNHLVGVKNSVPKSAA
                     DEVYALLLESKKAAGDPPMLPHGLEANRRNLEVAVDCAYKQNMIPRRFKVEELFE"
     gene            14567..15091
                     /locus_tag="VIG70_03265"
     CDS             14567..15091
                     /locus_tag="VIG70_03265"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY8249599.1"
                     /translation="MDQVQEVIEAPLSSEQLALRYRGLCEDLRFANLPGKIELDNWGR
                     LLMSPASNYHSAVQTALSGKLAALGGRAFVEASVLTSAGVLVADVAWASEEFMRVRGF
                     ETPYTRAPELCIEVVSPSNSRKELSEKVAAYLEAGAEEVWMLYPQSKRCEFHDKQGLV
                     QRSRYAVDLADIFS"
     gene            15116..16066
                     /locus_tag="VIG70_03270"
     CDS             15116..16066
                     /locus_tag="VIG70_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249600.1"
                     /translation="MKRLLAALLAAPLLAQAQAYPTKPVRIVLPFGPGGIADITTRTI
                     APKLSEGLGQQVIVENLPGAGGIRAAETVAKADPDGHTLLLLTNGNAVSQALFKSLPY
                     DPVNDFAMISTVGFFSMVIVTRPESQAKTLQEAIAMAKAHPGKMNIGTITPGGTQHLA
                     GELFRSTAGIDALVIPHKTTGEVIVAARNGNVDLAVDFIAPLLSNIKSGALRALAVTA
                     GKRFPGLPDVPTAIEAGVAGYDVASWNAMAAPAKTPRAVVDRVNQELRKAIASPEVQK
                     RFEELGVDGRTSTPDQLREFFVAESQRWTRVVEAAKIPKQ"
     gene            16066..17580
                     /locus_tag="VIG70_03275"
     CDS             16066..17580
                     /locus_tag="VIG70_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY8249601.1"
                     /translation="MSALRGADVLARTLAAAGTRYVFTLSGNHVMPVFDAALDAGLDL
                     VHVRHEAAAVHMADAWGRLTGEPGIALVTGGPGHANALGALYTALAAQSPLVLISGHA
                     PLAELGRGAFQEMAQAELAAPLTKLSRTVQSAQSLGAELARALREARSSPAGPVHLSV
                     PNDVLDARLEASIPARQELLTPAQTYSTEEAESLLELLGRAERPVIVCGPGGAHGRGR
                     ARLQELEDASGVPVIVMESPRGVNDPALGRLAAVLARADLALLAGKRRDFTLRFGAAF
                     GDGCRVLEVPPALVARLRPPRRLNSGGWLDEARARLAYRPPEWNAIQDSLHPVVVCRA
                     AQEILSGPDAILVCDGGEFGQWAQACMRAPQRVINGPAGSIGSALPFAAAAKLAFPAA
                     PVVAMLGDGTFGFHMSEIDTAVRRGLAYVAVIGNDACWNAEYQIALRAYGAERAHSLD
                     LLPTRYGAVAAAMGAHGEDVSSSSQLQGALARAAQAGKPACVNVMIERAAAPRY"
     gene            complement(17632..18096)
                     /locus_tag="VIG70_03280"
     CDS             complement(17632..18096)
                     /locus_tag="VIG70_03280"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY8249602.1"
                     /translation="MAVRLENRVPPPIVAACVGALMLAAAWALPMLNVEIPGATLAAI
                     LVATASVTIALAGVIQFARAGTTVNPLQPDAASSLVDGGVYRWTRNPMYAGMAGVLLA
                     WAIYLSSIAALAVLPLFVLYLNRFQIVPEERALEARFGAEFERYRNEVRRWL"
     gene            complement(18081..>18344)
                     /locus_tag="VIG70_03285"
     CDS             complement(18081..>18344)
                     /locus_tag="VIG70_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249603.1"
                     /translation="HSMRLAAGQEALVARALTQDGKTGFGFSFRLDGAEARHMAEWHA
                     GARTEPPPLDPVLDHPWEKAFLEGKPIPWDCEPGFAGLTWLSG"
BASE COUNT         3095 a         6117 c         6223 g         2909 t
ORIGIN      
        1 gagcttgagc aggcgctgcg cgttgccgtg caggatcttc gcgcggtcct cggggccgag
       61 cgggacgtgc tccatccact tgacgcccgg cgggttgggc acgaacgggt agtcgaccga
      121 gaacaggatc cggtcggcgc ccatttccat cacgcagcag agcagcgacg gcgtcgagaa
      181 gttgccgctg gtggtaatcc agaagtgttc gcgaaaggtg tcgcggaagg gcgtcttgcg
      241 gttgccctgg cgcgcgagcg ccatgtcgat gcgccagagg tagaagggca ggccctcgcc
      301 gaggtggccg aggatgatct tcaatcccgg atacgcctcg aatacgcccg acagcaccag
      361 ccggattccc tgcgtggcgg tctcgacgcc gaagccccag gccgcggtca tcaggccggg
      421 aaactgttcc aaataatccc ggtagtagac atcgatcacc gccttgtgcg ggaccgcggg
      481 atgcagatag agcgggacgt cgagcgcctg cgcgcgctcg aagatcggcc agaagcgctt
      541 gtcgtcgatg aacagattat tcgtaagacc atgaatcatg gcgcccttga agccgagccg
      601 cgtcaccgcg cgctcgagct cgtcggcggc agccttgggg tcggcgctcg gcagcacggc
      661 gaagccctcg aatcttccgg gatgcttgct tacggcttca tgcaggcggt cgttggcgcg
      721 ccgcgcaagc ggcaccgcgg tctcggggtc gaggcgctgc gttgccggcg cgccgtgcga
      781 gagcacttgc aggtcgatgc cggcctcgtc catttccttg atgcgcagcg cgccgaggtc
      841 gaacaggcgc tcctgcagcg cggggatgcg catctccggg ccgcgctcct tgaaatgggt
      901 cgcgacttcc gggtcccagt agtgttcttc gagggcgatg attttcatag gagaaaccat
      961 cttaccccgg cgagcgagat ctgcggaacg taggtgatga gggcgagcgc gccgagatag
     1021 atcacgatga agggcacgat gccgcggtag atcacctcga gcttcaggcc gagcgccgac
     1081 tgcgcgacga agatgttgat gccgaagggc ggatggaaca ggccgatggc gagattcacc
     1141 gtgaccacga tgccgaagtg gaccgtgtcg atgccgagcg ccttgaccac cgggatcaag
     1201 agcggcgtga ggagcaggat cgccgacagc ggatcgagga agcagccgac caggagcagg
     1261 aggacgttgg ccaccaggag gaattctcct tgtgaaacgc cgagagactg tatccatgcc
     1321 accagcgcct gcggtacctg ctgcaccgtg aggagccacg cgaatacgcc cgcgcaggcg
     1381 acgatgatca gcacctgtcc ggtaaatagt gcagtgctcg cggccgcttc caccacgtcg
     1441 ctccagccga gctcgcgaaa cactacccag gtgacgagcg ccgcgtagac gcaggccacc
     1501 gcggccgcct ccgtggggga gaagatgccg ccatagatgc cgccgaggat gatgaacggc
     1561 gcgccgagcg cccacgcgcc gcgtgccgcg gctccgagaa aagcagacag ctgaaaaggt
     1621 gagccgactt caaaaccctg ccgcctggcg gtccacacta cgtagatcgc gaggagcccc
     1681 gcgatcagca gccccggcag cacgcctgcg gcatagaggc gcggcaccga ctcctgcgcc
     1741 gccatgccgt agacgatgag cggaatgctc ggcggaatga tgatgtcgat cgcgccgacc
     1801 gcggtgatca gcccggcggc gaagggctcc ggataacccg cgcgccgcat cgcgggaagc
     1861 atcgccttgc cgaccgtggc cacggtcgcc gcgctcgcgc ccgagatcgc gccgaagatc
     1921 gccgaggtgc ccacggtcgt aacgccgagg cttcccggca cccggccgac ccccgcctgc
     1981 acgaaatcga cgatgcgctg cgccacgctg ccgcggctca tgagctcgcc cgcatagatg
     2041 aagaacggca ccgcgagcag cgcgaaggcg tcgatcgcgc cgaacaggtt ctggtgcagc
     2101 acgaccagcg ggacgttcat atagaagatg agcgccacgc tcaccgcggt gagcaggacc
     2161 aggtagatcg ggaagccgag cagcagcagg gcgaccggca ataccccgag ggcgaaactc
     2221 atttcgagtt tcttagggaa actatcaata tgaggaagaa ccccagggtc acggccccat
     2281 gcggaatcca catcgggatg ccgccgacat cgctgctgcg cccgagggcg agcatctgcg
     2341 cgctgtagcg ccaggattgc accaggacga agcccgcgac ggcggcgacc aggacgattt
     2401 cgcaaatgcg cagcgccgat tgcagtccgg gcggtagaaa gcgcaccagc acgtccatgc
     2461 gcaggtgctg gcgccgccac gacaccaccg ccgcgcccag gaacgccatg aacaccatga
     2521 tgtagatctg gatttcgtcg gcggcggcga tcgagcggcc gaagccgtag cggccgacca
     2581 cgttggcgaa gttgaggcag accgcggcga taaaggcgag cgccaggatg cgctcgatcg
     2641 ccgtcatcag ccggttcatg gtcaaatcct aaccatgagg aggtttcgat gctcgtaaag
     2701 ctctttgccg ggttatttgc cggcgctatc gcgttcgcgg cgccactcgc cggcgcgcag
     2761 caatatccgt cgaagccggt gcgcgtgatc gtgccgttcg cgccgggggg cggctcggac
     2821 ttcaccgcac gcctcatggc gcagaagctg accgagcgcc tcggcaaccc gttcgtggtg
     2881 gaaaaccgtg ccggcgcggg cggcaacctc ggcgcggaga tggcggtgcg ctcgcccgcg
     2941 gacggctaca cgctgctgct catctcggcg agctacacgg tgaacccgag cgtgtacaag
     3001 ctctcgttcg attcggtcca ggacatcgcg ccgatcatcc agatctccgg cgggccctac
     3061 gtggtggcgg tgcatccctc agtccccgcg aagacgctcg cggaattcgt tgcctacgcg
     3121 cgccagcagc cggagaagct cgcgtacggg tcttccggca acggcagcgt gatgcacgtc
     3181 tccaccgcgt acttcctcga atcggccagg atcaaggtgc tgcacattcc ttacaagggc
     3241 accggccctg cgctgcaaga caccatcggc ggccaggtgc agctcgtctt cggcgcggtg
     3301 ccgaccacgc tgccgcacgt gagagccggc aagctgcgcg ccctggcggt gacgacggcg
     3361 aagcgcatcg ccgccgcgcc cgagattccc accatcgccg agtccggcta tcccgactac
     3421 gaggtgacga actggcacgg cctggtcgcg cccaagggag ctccgcgcga ggtcgtcgag
     3481 cgcctgaaca aagagatcaa cgagatgctg accagcgaag acttcaggaa ggtgctggcg
     3541 aacgacgggc tcgagcccgc gggcggctcg cccgcccggt tcggcgaggt cctcgcgaag
     3601 gaaaccgcgc gctggaaggc tgtcgtgcaa caggcaggca tcaaggtgga gtgaagacaa
     3661 accaccgcat cgacggccgc gacggccgcg gcggccgctg ggtgactttc gtcaccggca
     3721 tcgccaacga tctcaccatg tgggacggcc aggtccccgc gctcgcgggc gacttccgca
     3781 tcctgcgcta cgacctgcgc ggccacggcg gcagcgaggc aacgggcggc gattacagca
     3841 tggcgctctt ggttgaagat ttgaaagcgc ttctcgacgc acagaaaatt caaaagacct
     3901 ctctggtcgg tctcgggctc ggcggcgcgc tcgcccaggc gttcgcgatc gagcacccgg
     3961 atcgcctgga ccgcctcatg ccgtgctgct gccgcgcgcg catggtcccc gacttcgccg
     4021 cgatgtggca caagctgcgc gagacggtgc ggcagaacgg gctcgagacg atcgtcgagc
     4081 cgaccgtgca gcgctggttc tccgaggact tcaaggctgc gcatcccgac gtgctcgaca
     4141 acattcgcag gatgatccgc ggtacgaccc tcgagggcta cctcggcgtt accgccgcat
     4201 tccttggcct cgacgtggag gaccggctgc aggagatccg ggccccgacc ctctacgtga
     4261 gcggcgccga ggacaagctc ggcgggccgc cggcgctcat gcagggattg agcggcaagg
     4321 tgaaaggcgc ccgccacctc tcggttccaa aggcggcgca tatcgctaat attcagaacc
     4381 cggaggggtt caatcgcgtg ctgggggagt tcctgaaatg agactttttc tggtcctgct
     4441 gttcttttgc agcgcggcgg gcgcacaacc gtacccgagt aagccgctgc gctggatcgt
     4501 gccgaccggc cccggcagcg cggtcgacgt gaacgcacgg cgcgtgaccc cgaagctcgc
     4561 cgaagcgctc ggccagccgg tgatcgtcga gaaccggccc ggggcgaaca gcatgatcgc
     4621 ggcgcgcgaa gccgcgcgcg cggcgccgga cggctacacg ctgttccagg cggtggtgaa
     4681 caacgcgatc aacgacgcgc tcgcgccgga tccgtgctgc gtgctgaacg acaagctcgt
     4741 gccggtgacg cgcctctata cgacgccgct cgtgatggtg gtgcacccct cgcttcctgg
     4801 aaatctcaag gattatgtgg cgctggcgaa atcgaagccg aactcgctca cctacgcgca
     4861 tggcgggacc ggcgcgatca cgcagatggt gggcgagctc ctcaagctca ccgcgggcat
     4921 ccaggtgcgc gaggttccgt acaaggcgat cggcgccgag cttcccgacc tcaccggcgg
     4981 gcacgtcagc accgcgttcc tctccccggt ggtgatccgc gacatggtgc gcgcgggcaa
     5041 gctgcgcgcg ctcgcgattg ccgaccgcca gcggctcgac atcctgccgg aggtgccgac
     5101 gatggcggag ggcggctacc cgatcgaggg gctgggctgg aacggcatct tcgtgccggc
     5161 gggcacgccc cggcccgtgg tcgatcgcct ccacaaggag atcacgcgcg tgctgcaact
     5221 gaaggaaatc caggatgacg ccatggcgct cggcacgcgc gcgggcggcg agaaacccga
     5281 ggagttcgcc gcctttgtac gctcggaggt gcagaagtgg ggcaaggtca tcaaggacgc
     5341 cgccatcaag gctcagtagg gaacagggga catacatata tgaagctctt ttcgctcatc
     5401 gccggtcttt tcctgacagc cgcttgctac gcgcaagcct ggcctaacaa gccggtccgc
     5461 gtgatcgtga acgtcgcggc gggcggcgtg gccgacgtca ccatgcgcgt gctcggcgcg
     5521 cgcatgaccg agacgctcgg ccagtcgttc ctggtcgaga accgcgccgg cggcgacggc
     5581 tacatcggct tcgacgcggt ggcgcgctcc gacccggacg gccacacgct gctctactcg
     5641 cccggctcga gcatgatgat cgcgccgcat atcgtgaacc ggcccgatct cgatcccctc
     5701 aaggtgctgg tgccgatcgc cgcctccggg cgcgtatcgc tctacgtgat cgcgcacccc
     5761 gagcggccgt tcaagaacct gaacgagctc ctcgcctatg cccgcgccaa tcccggcaag
     5821 ctcaactacg gcacgccggg caacggcact tcgccgcaca ttgcgaccga ggtcttcaac
     5881 cgcgaggcga aagtgaagct gaaccatgtg ccgtacaaag gtgccggccc ggcgctgcag
     5941 gacctgatcg gcgggcagat cgacatcgtg ttcgatcccg gcgtcggcat cgcgccagcc
     6001 aaggccggca aggtgcgcat gatcgcgatt gcggggccga agcgccatcc cgactttccc
     6061 gacatcccga cgctggacga gaacgggatc aagggcgtcg acggcgggcc ccatttcggc
     6121 ctttacgggc cggccggaac gccccgcgat gtggtcctca gaataaattc ggagctactg
     6181 aaggccatgc agcacccggc ggtggccgag cgcttcaagg cgctcgccgt cgacgtggcc
     6241 gagcccatga gccccgagca gttcgccgcc tacgtgcgcg gcgagcacgc gcgctacgcc
     6301 aagctgattc ccgagctcgg gatcaagacc cagtaaagcg gaacacgcgc tcggcagggc
     6361 cggtcttcgc cgtatctttt ttcgacaggc cccagtggtc gatgcggccc ggcggccact
     6421 tccagtcctc cgggccgccg accttgtagt cgtcccccac ctcgctcacc tcggcggtgt
     6481 actcgatcac gccgccgtag ggcgcgatga agtagccgaa cacgttgttt cccgggccgt
     6541 gccgcccggg cccccagacc ggctcgaatc cggcctcgcg cacgcgggcc atgccgcgca
     6601 tcaccgcgtc gaggctcggc atctcgaagg cgatgtggtt gagcgaggcg agctccgatt
     6661 tcgcgtaggc gacgcagtgg tgcttgcggt tgcagcgcag gaagcgcatg tgcgcggtgc
     6721 ggtcggagac ctggaagccg agcttctcca ccgcgaagcg ctcggcagct tccacgtcgg
     6781 tcgagttcac caccgcgtgc gtgagctgga tgggcttgtc cgcgtctttc aaccgctccg
     6841 gaacgccggt ctcttcgatg aagcgatagg tttgcgcctc ggggccgcga acctcgaagc
     6901 cctcgccgcc gcccggcacg ttgaacttga tcattcgcgg cgatccgttg cgctgcctca
     6961 ccgagtgaag ctcgtccgga gcgccggaaa acgtgatcgc cgccaccgcg ggcgccgagg
     7021 ctctggtgat cgacaggatg tacggatgat tcccggtacc gcggaagaat tgcgtcgcgc
     7081 cggacgtgcc tgccggcgcc aggccccaga cgctgccgag gaaatccgcc gcctgcgcga
     7141 catccggcag ctcgagctcc acgctgcgta gtttcatttc agaccttttg ccatttcctt
     7201 gagcatgcgg tcaagcgcag cgtcgtcgcg cgctacgccg tatacatagt gatccggccg
     7261 cacgatggcg gcagtgcaga tgttcttgtc gaaccagtcg gcgaccaagg tttggctcgg
     7321 gtcacgcttc accgtccgca ttcccgtgcc gccgtccttc ggaaacaggg tgccgtttgc
     7381 cgcgtggggc cgcgatgaga gaaacccatc cttgagcggc gggatcaggt gctggcgcgg
     7441 cacggtgacg accttgccgc cggtttcggc cagatgccag cgatcgcgcg cccgcgcctt
     7501 ttccacgttg cgctcgccga tgacggctcc gatgtccttc acgatcgaaa tcagccgttt
     7561 cgcgtgcgcg ctgcgctcgg cttcgtaggt gtccagcagc ctctctttcg ccttgccggc
     7621 gagcacgtag tgcagcttcc agctcaggtt ggccgcatcg cgaatcccct ggcacatccc
     7681 ctggccggtg aacggcggct gctggtgcgc ggcatcgccg gcgaggaaca cgcgcccgcg
     7741 ccgccactcg cgcgcgacga gcgcgtggaa gcgataggag gccgcgcgcc agagctgcgc
     7801 atcgtcgggc gtgatccagc gcgcgagcaa cttccagaca tcgggctctt cgcccggatc
     7861 gagcatcagc tcccagcgcc ggtgcgcgcc cacgccgatc aggtaggtgg aagggcgcgc
     7921 cggctcgcag tactggatgc tgaccttcgg cagcttctcg agcccgcgcg ggctcaccag
     7981 cagatcgacc accagccagg gctggtcgaa atcgagatcc tcgtactcga tgccgagagc
     8041 gccgcgcacc gtgctgctgg cgccgtcgca ggcgacgaca tacttcgccg tgatggtgcg
     8101 atcgcgcagc cgcagcttca cgtccttttt cgacgggtcg aggccctcga gctcggtgaa
     8161 ggcaacctcg gcgaccgccg cggcgcggtc gcgcagcagc gcgtcgaccg cgggctggtt
     8221 gaacaccatg ctgggcggcc agccgagcgg ccagggcgga ggcagcgcgc cgaggcgctt
     8281 gatgagctgg ccgtcgacgc cgtaatactc ggaggacgtg aaaagcgcgg tatgcggcgc
     8341 cacggcttcg gcgatgccga tgtcttcgaa cacgcgcatg atttcgtgat cgagggcgaa
     8401 cgcacgcggc ttgtcgtaca ctgcctgctc gcgctcgatg gcgatggtct tcaggccgag
     8461 gccgccgagc agggcggtgc agaccgcgcc ggtggggccg aggccgacga cggccacatc
     8521 gaagctatca ttcacctgcc gaagcatgcc atttaaaccg ccgccgctcg cctgtgatgc
     8581 gcattgccac gtgttcggcc cggcggcgaa gttcccctac gaccccaagg ctgcctacat
     8641 tccggcggat gcgccgttcg aggctttgca gaaattgcat ggacggctgg gaatcgaaag
     8701 ggcggtgatt gtccacgcga gctgccacgg ggccgacatg cgcgtcacgc tggatgcgat
     8761 cgcgcgctcg ggcgggcgct accggggcac ggccatcatc gacgagtcct acggtgatcc
     8821 ggaattcagg aagatgcacg acggcgggat ccgcggcgtg cgcttcaact tcgtcaggca
     8881 cctcggcggc cgtcccgaaa tgggcttttt cacgcggacc gtcgagcggg tgcaggcgat
     8941 gggctggcac ctgatcctgc atctcgacgc ggcggacctg gtcgagttcg acgcgttgtt
     9001 ccgcaagatc cgcgcgccca tggtcatcga ccacatgggc cgggtgaagg ccgcggacgg
     9061 gttgcagcag aagccgttcc aggtgctgct cgagtggatg aagcaggaca acttctgggt
     9121 aaagatttgc ggcgcggaac gcgtctcctc gaaagggccg ccgttcaccg acgcggtgcc
     9181 gttcggccgc gccctggtcg aggccgcgcc ggagcgcgtg ctctggggca ccgactggcc
     9241 gcatcccaac gtgaagtgga tgcccgacga tggtgcgctt gtggagctct tcccactgat
     9301 ggtgccggag ccggcgctgc agcagcgcat cttggtggac aatccggagt cgttgtacgg
     9361 cttccaaggg agatgaccat gctggggctc accacgctgg gcgcgatcca taccacaatc
     9421 agcctgatcg ccctcgccgc gggattcgtc gcgctcgcgc gccactggga gatctcgaac
     9481 gcgagccggt ccggcaaggt tttcgtgtgg gggacggtcc tgtcgtgcct gaccggcctc
     9541 ggcatcttcc agcacggcgg cttcggcaat ccccacatcc tggcgatcgt cacgctcgtg
     9601 gtgatggcgg tcgctctcgc cgcgcagtac accgccgtgt tcggtcgcct gtcgcgctac
     9661 gtgagcacca tcggctattc gctgacgctc ttcttccact tcatcccggg cacggtggag
     9721 acactcacac gcctgcccgt gggcgcgccg tatctctcgg gcccgctcga tccgaaagcg
     9781 cagccgatcc tcggcttttt cttcctgctg ttcctgatag gcgcaacgct gcaggtggtg
     9841 cggctgcgcg ccgggcgcga gaccgagccg ctgctcgccg atcggcagga agagtcctag
     9901 gtcagatcgt atttccgagg cgcacggaac ggcgcaccag ctcctcccag agtccgtcgg
     9961 ggttgcgcat gaagagcgcc gcgtcgggct cgagcacgta ccaggcgcct tgcttgatct
    10021 cgtccttcag ctcgcccgcg cgccaggcga ccaggccggc gacgaagcgc gcgcgcgtcg
    10081 ggtccgattc aatgatcctg tccaccacct cggcatcgaa ggccgcgaac aggccgggca
    10141 tcatctcgaa ggagctgccg cccgggttgt cgcgccgctg caccaaggcg aacacgacct
    10201 gggactggat cggcccgccg agatagaccg gatcgacgat cttttgcgag ggcccgtgct
    10261 cggggaacag gctcccgagc gtgacgcggg tgggccggtt gacgatgaat cccgcatgct
    10321 ggccgccgcc gaccggcgcc acgaccagga cgctcgagcc gtagagcttg tcctgcagct
    10381 ccggttttgc caccaggatc agcggccggt cgaggtcggc agcgccggcg agcgggatcc
    10441 agccgaggca gaaggcgaga agggcgaggg cggcgttgcg catggtcatc tcccagagca
    10501 tgaggcgatg cttgcgcacc agcgtttgcg ccgtgctacg gcggcccgcc caggtgttac
    10561 gaatgaaacc tgcctactcc atgaaccatc catggctcac gatgagcgac tgccccgtga
    10621 gcgcgttggt cttgaacgcc gcgagaaaga gcgcggcttc cgccacgtct tgaacggtgg
    10681 tgaattcgcc gtcgacggtt tccttgagca tcacgttctt gatgacctcc gcttcggtga
    10741 tgcccagttc tttcgcttgc tccggaatct gcttgtcgac gagcggcgtg cgcacgaagc
    10801 ccggacagat gacgttggcg cgcacgccgt gcttggcgcc ttccttggcc accaccttgg
    10861 cgaggccgat gaggccgtgc ttggcggtga cgtaagggcc cttgagcaca gaggcctcct
    10921 tggagtgcac cgagcccatg tagatgacgg cgcccccccg gcccgacttg tacatgtgct
    10981 gcaggcaggc gcgggtggtg aggaaggcgc cgtcgagatg gatcgcgagc atcttcttcc
    11041 actcggcgta ggggaactgc tccagcggat gcacgatctg gatgccggca ttgctcacca
    11101 ggatgtcgac gccgccgaac gcggccacca tggctgcgac gccggcattg accgcgtcct
    11161 cgttcgccac gtccatcgcc acgccgatcg cctgcccacc ggtggccttg atttcggcgg
    11221 ccgcggcatc ggcggcgcgc ttgtcgaggt cgccgatcgc gaccttggca ccttcgcgcg
    11281 cgtaggcaag ggcgatctcc ttgccgatgc cgctggcggc gccggtgacc agggccgact
    11341 taccttgcag ttgcatgaga agtcctttct tcggtcatgt cctgggcgtc atgtcgaaaa
    11401 cgcgcaagcc ttcggccaga tcggtcgcgc gatgccattg ggggccggcg accgagcggc
    11461 gcacgtcgcc gaggccgcct tgccataggt cggtcacggt agcgcgggag aattcgcagt
    11521 ccttgaagct cgtggcgcgc gggtggtggc ggttgatgaa gtgcgccagg gcgcaagccc
    11581 tgggcttgct ggccgccgca aggcgttgca cgtcgggatc gtcgcgcagc gcttcgggca
    11641 gcttctcgag cacccggcgc agcgcgccgc gcagcgcttc gatctccttg acccggctgg
    11701 tattgaatcg cgtcttgctc gcgtactgga tgtccttcgc gcgctcctgc acctcggcga
    11761 gattcctcgg cgcctcgccg gcgccgctga acacgtcgac ctggaagatc aacgcgctca
    11821 tctcgggatc cgcgtcgagc acgtaccata gcggcgagtt cgacacgatg ccgccgtccc
    11881 agtacagctc gccgtcgatc tcgaccggcg ggaagcccgg cggcagcgcg ccgctcgcga
    11941 gcacgtgctc ggcgcggatc ggcatggtct tgctgtcgaa atacaccgaa ttgccggtgc
    12001 gcaggtcgac cgtgccgagg gaaagccgca ccccgttgcg gttgatgagc gcgaaatcga
    12061 ccagctcctc gagcgtcgcc ttgagcggct gcgtatcgta gaagctcagc gccccgatgc
    12121 tgccgtccgg ggcgaggaac ggcgggacca tccgcggcga gaagaatccg ggcacgccga
    12181 acacgaaagc cgacgccgtg ctcatctggt cgagcatcgg ccgcatcggg tccatcgccg
    12241 ccggcgggat gaacggcagc tgcgccgaga cgcgatccca gaattcgcgc aggcgcgcga
    12301 cgcgccgttc gggcggattg cccgcgatca gcgcggcgtt gatggcgccg atcgagacgc
    12361 cggcgaccca gtcgggcgcc gcgccgcact cggcgaggcc ttcgtagacg ccggcctggt
    12421 aggcgccgag cgcgccgccg ccctgcaata cgaggacggc atggtcatac ttcccccagt
    12481 tggcggtcac cagcgcgaaa ccatggccgg catgctgtgg gctggcatgc gggtgggcaa
    12541 ttggactttg gtcccatgcc agtagaatcg ccggcatggc acgcatcatc ggcgggatcg
    12601 ggacgtccca cgtcccgacc atcggcatcg cgtacgacaa gggcaagcag gaagatccgg
    12661 actggaagcc gctcttccgg ggctacgagc cggtggcgaa gtggctcgcc gagaagaagc
    12721 ccgacgtgct cttcttctgc ttcaacgacc acgccaccac gttcttcttc gatcactacc
    12781 cgacgttcgc gctcggggtg agcgacaagt accgcatcgc agacgagggt ctgggggtca
    12841 ggaacattcc tccgttgagg ggccacgctg cgctggcgcg ccacatcgcc acgtcgctcg
    12901 tgaacgagga gttcgacctg accctgttcc aggatctgcc gctcgatcac ggcctgcact
    12961 cgccgctcac catgatgtgg ccgaaaagcc cggactggcc tggcgcggtg attccgctgg
    13021 tggtgaacgt gctgcagcat ccgctgccga cgccgcggcg ctgctacaag ctgggcaagg
    13081 cgatccgcag ggcggtcgaa agctttcccg agaacctgaa ggtcgcgatg gtcggcaccg
    13141 gcggcctgtc gcaccagatg atcggcgagc gcgcggggtt caacgacgaa gcctgggacc
    13201 gcgagttcct ggatctcatc gagcgcgatc ccgagcgcct ggcgagcatg accatcgagg
    13261 atttcatgcg acgcggcggc aacgagggcg ccgaggtgat catgtggctg atcatgcgcg
    13321 gcgcgctgga cgagcgcgcg aagaagctgc accagaacta ctacctgccg atgaccacgg
    13381 cgatggcggt cgcgctgttc gaatgaagta tgttttcgat ctggacagaa gcgcgcgcgg
    13441 gctgcggctc aaccggttcc tgcacggcct gacttcgaag gaagcgagga cgcttttcct
    13501 gagcaagccc gaggaggcga tgcagggtct gaccgagccg gagaaagaca tggtgcgccg
    13561 cctggactgg aaggcgatgc aggactacgg cgcgagcttc ttctgcctgg agaagcttgc
    13621 gcgcaccaag ggcgtgtcga accccgagat ggtcgcgcac ttccgcggcg agacgctgga
    13681 gcagttcctg aagacccgcc gggtccccgg agcgcggtag tgaagctcaa gaccctgatc
    13741 agcgactatc cggtcaccaa ggaattccgc acgcacacgg agcgcttcga gttcgtgccg
    13801 ttccagggcg gcgtggcgca gcagttccag cgcgtggtgc gcaagctgga gttcgacgtt
    13861 gccgagatcg cgctgatgac cttcctcatc gccaaggcgc acggcaagcc ctaccggctg
    13921 ctgcccttca ccgtcatggc gcgctaccag catccgttcc tcaagttcaa cccggagcgc
    13981 acgcccggac tgaagcccgg cgacctggcg ggcaagcggg tcggcgtgcg ctcgtactcg
    14041 gtcaccaccg gcgcctggat ccgccacatc ctcgccggcg actacggcgt cgacctggcg
    14101 aagatcagat gggtcacctt cgaagaggcg cacgtcgcgg agttcgtcga tccgccgaac
    14161 gtcgagcgcg cgaccgccgg caaggagatc aaggcgatgc tgctcgccgg tgagctcgat
    14221 gccgccatcc tcggcgactc cacgcccgac ccgcggctgc agccgctgat cggcgatgcc
    14281 gaggcggccg gccggcgctg gagcgaaaag caccacgcga tccagctcaa ccacctggtc
    14341 ggggtaaaga attcggtgcc gaagtcggcc gccgacgagg tctatgcgtt gctcctcgag
    14401 agcaagaagg cggccggcga tccgccgatg ctgccgcacg ggctggaagc gaaccggcgc
    14461 aacctggagg tcgccgtcga ctgcgcctac aagcagaaca tgattccgcg gcggttcaag
    14521 gtcgaggagc tctttgagtg aatgtgcgtc aagtagaatg attttcgtgg accaagtgca
    14581 ggaagtaatc gaagcgccgc tctcgagcga gcaactggcg ctgcgctacc gcgggctttg
    14641 cgaggacctg cgcttcgcaa atctcccggg aaagatcgag ctcgataact ggggccgtct
    14701 cctgatgagc ccggcgagca actaccatag cgccgtgcaa accgcgttgt caggaaagct
    14761 ggctgccctg ggtggccggg cgttcgtcga ggcatcggtt ctcacctccg ccggcgtgct
    14821 cgtcgcggat gtggcctggg cgagcgagga gttcatgcgt gtccgtgggt tcgagacgcc
    14881 gtacactcgg gcgccggagc tatgcatcga agtggtatcg ccatcgaatt cgcgaaaaga
    14941 gctcagcgaa aaagtggcgg catacctcga agcaggtgcc gaagaggtgt ggatgcttta
    15001 cccgcagtcg aaacgatgcg agttccatga caagcagggg ctcgtgcagc gctcgcgcta
    15061 cgcggtcgat cttgcggata tctttagctg acgtagagcc gaggaggagc tccgaatgaa
    15121 aagactgctg gctgcgctgc tcgctgcgcc gttgctcgcg caggcgcagg cctatccgac
    15181 caagccggtg cgcatcgttc tgcccttcgg cccgggcgga atcgccgaca tcaccacgcg
    15241 caccatcgcg cccaagctgt ccgagggact cggccagcag gtgatcgtcg agaacctgcc
    15301 cggcgcgggg ggcatccgcg cggccgagac ggtggccaag gccgaccccg acgggcacac
    15361 gctgctcctg ctcaccaacg gcaacgccgt gagccaggcg ctcttcaagt ctctgcccta
    15421 cgacccggtg aacgatttcg ccatgatctc gaccgtgggc ttcttcagca tggtgatcgt
    15481 cacgcggccc gagtcgcagg cgaaaacgct ccaggaagcg atcgcgatgg cgaaagccca
    15541 tccgggcaag atgaacatcg gcaccatcac gccgggcggt acgcagcacc tcgcgggcga
    15601 gcttttccgc tcgaccgcgg gtatcgatgc gctggtgatt ccgcacaaga cgaccggcga
    15661 agtgatcgtc gccgcgcgca acggcaacgt cgacctggcg gtggatttca tcgcgccgct
    15721 cctctcgaac atcaagtccg gcgcgctgcg ggcgctcgcg gtcaccgccg ggaagcgctt
    15781 ccccggattg cccgacgtgc cgacggcgat cgaggcgggc gtcgccggct acgacgtcgc
    15841 ctcgtggaac gcgatggcgg cgccggcgaa gacgccgcgc gcggtggtcg accgggtgaa
    15901 ccaggagctc cggaaagcga tcgcgtcgcc cgaggtgcaa aagcgcttcg aggagctcgg
    15961 ggtcgacggg cgcaccagca cgccggacca gctccgcgag tttttcgtcg ccgagtcgca
    16021 gcgctggacc cgggtcgtcg aggcggccaa gatcccaaag cagtagtgag cgcgctgcgc
    16081 ggtgccgacg tgctggcgcg tacgctcgcg gcggcgggca cgcgctacgt tttcactctg
    16141 tccggcaacc acgtcatgcc ggtgttcgat gcggcgctcg acgccgggct cgatctcgtg
    16201 cacgtgcgcc acgaggcggc cgcagtgcac atggcggacg cgtggggaag gctgaccggc
    16261 gagcccggca tcgcgctggt caccggcggg cccgggcacg ccaacgcgct cggcgcgctc
    16321 tacaccgcgc tcgccgctca atcaccgctc gttctgattt ccggccacgc gccgctcgcc
    16381 gaactgggcc gcggcgcatt ccaggaaatg gcgcaggccg agctcgctgc gccgcttacc
    16441 aagctctcgc gcaccgtgca gtcggcgcag tcgctcggcg cggagctcgc gcgcgcgctg
    16501 cgcgaggcgc gcagctcgcc cgcgggaccg gtgcatcttt cggttccgaa cgacgtgctc
    16561 gacgcgcggc tcgaggcaag catcccggca cggcaagagc ttcttactcc tgcccagacg
    16621 tactccaccg aggaagcgga aagcctgctc gagctgctgg gccgggctga gcgccccgta
    16681 atcgtctgcg gcccgggcgg cgcgcacggc cgcggccgcg cccgcctgca ggagctcgag
    16741 gacgcgagcg gcgttccggt gatcgtgatg gagagcccgc gcggcgtgaa cgacccggcg
    16801 ctcgggcgct tggccgccgt gctcgcgcgc gccgatctcg cgctgctcgc cggcaagcgg
    16861 cgcgacttca cgctgcggtt cggcgctgcc ttcggcgacg gctgccgggt cctcgaggtg
    16921 ccccccgcgc tcgtcgcaag gctgcgcccg ccgcgccggc tcaactccgg cgggtggctc
    16981 gacgaggcgc gcgcgcggct cgcctaccgg ccgccggagt ggaacgcgat ccaggactcg
    17041 ctgcatccgg tcgtggtgtg ccgggcggcg caggagatac tctccgggcc cgacgcgatt
    17101 ctggtctgcg acggtggcga gttcgggcag tgggcgcagg cctgcatgcg ggcgccgcaa
    17161 cgcgtcatca acggccccgc gggctcgatc ggatcggcgc tgccgttcgc ggccgcggcg
    17221 aagctcgctt tccccgcggc gccggtggtg gcaatgctcg gcgacggcac cttcggcttc
    17281 cacatgtcgg agatcgacac cgcggtgcgc cgcggtctcg cctacgtcgc ggtcatcggc
    17341 aacgacgcct gctggaacgc cgagtaccag attgcgttgc gcgcgtacgg cgctgaacgc
    17401 gcgcacagcc tcgacctgct tccgacgcgc tacggcgcag tggccgccgc catgggcgcg
    17461 catggcgaag acgtgtcttc ttcctcgcag ctccagggcg ctctcgcgcg cgcggcgcaa
    17521 gcgggcaagc cggcctgcgt caacgtgatg atcgagcgcg ccgcagcgcc gcgctactaa
    17581 agcccgcctc ttggcgaatg ccgagcgtgc cgaccagcag ttcccccggc ttcacagcca
    17641 gcggcgcact tcgttgcggt agcgctcgaa ttccgcgccg aagcgcgctt ccagcgcgcg
    17701 ctcctccggg acgatctgga agcgattcag gtaaagcacg aagagcggca gcaccgcgag
    17761 cgcggcgatg ctcgagagat agatcgccca tgcgagcagg acgcccgcca tgcctgcgta
    17821 catcgggttg cgcgtccagc ggtaaacacc gccgtctacg agcgacgaag cggcgtccgg
    17881 ctgcagtggg ttgactgtcg tccctgcgcg cgcgaactgg ataacgcccg ccagcgcgat
    17941 cgtcacgctc gccgtcgcaa cgaggatcgc cgcaagcgtc gcgccgggga tttctacatt
    18001 cagcatcggc agcgcccacg cagcggcgag catgagcgcg ccgacgcacg ccgcgacgat
    18061 cggcggcggt acgcggttct ctaaccggac agccacgtga ggccggcgaa gcccggctcg
    18121 cagtcccagg ggatcggctt tccttcgagg aacgctttct cccacgggtg atcgagcacc
    18181 ggatcgagcg gcggcggctc ggtgcgcgcg ccggcatgcc actcggccat gtggcgcgct
    18241 tcggcgccgt cgagccggaa gctgaagccg aaaccggtct ttccgtcctg cgtcagcgcg
    18301 cgcgcgacga gcgcttcctg gcccgccgcg aggcgcatgg aatg
//
LOCUS       DATBSZ010000050         3700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_6551, whole genome shotgun sequence.
ACCESSION   DATBSZ010000050 DATBSZ010000000
VERSION     DATBSZ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6551"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VIG70_03290"
     CDS             complement(<1..883)
                     /locus_tag="VIG70_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8249604.1"
                     /translation="MELTRIADHVARGADPTDEQFRLLVSSVTDCAIYLLDVSGRVAS
                     WNAGAERIKGYRAGEVTGRHFSLFYTPEDRAAGKPEQALGHAAREGRFQGEGWRVRKD
                     GTRFWAEVTITALRGAGGALGGYAKVTRDMTARHFEREREQLFAATFDSAANGITVAD
                     ITGRYLGANASFLRLVGYAEAELREKSIFDLTHPEDIAESWRAFQAILTGEADRLELE
                     KRFVRKDGAVLWVRASVTRIVDREGTVTRVVAQVDDITERRAADQRLRESEERLHAFT
                     SHSPSAMFLKRADGRYRF"
     gene            911..1282
                     /locus_tag="VIG70_03295"
     CDS             911..1282
                     /locus_tag="VIG70_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8249605.1"
                     /translation="MSGRILVVEDEEVMRELLRLHLSSAGYAVELAEDAIGAGYAVLK
                     TLPDLIICDVEMPHMDGFQFIAALRADKTLPRLPVIFLTSVVEGEGAARVELGAADYL
                     FKPVRLEELLAAVSRQLRPQR"
     gene            complement(1227..1448)
                     /locus_tag="VIG70_03300"
     CDS             complement(1227..1448)
                     /locus_tag="VIG70_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249606.1"
                     /translation="MQSKQEILTKLAQIQEQAQLTLDELPLRLAKDRLRLIIGLARYL
                     STTVEINWEEIPSAAAEAGATRPPAAPRA"
     gene            1545..2780
                     /locus_tag="VIG70_03305"
     CDS             1545..2780
                     /locus_tag="VIG70_03305"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEY8249607.1"
                     /translation="MLETSKAVVSEAAVSIADIRAAAARIAGAIEHTPCVHSRTLSKL
                     TGAEVFLKFENQQFTASFKERGALNKLLSLGAAERSRGVIAMSAGNHAQAVAYHAARL
                     GIPALIVMPKGSPNTKIRNTQIHGATVVLEGENLFEAGKHARALAARDNLVFVHPYED
                     PLIIAGQGTVGLEMLEAVPDLEVLVVPVGGGGLISGVATAAKALRSSIEVYGVESKNY
                     PSMHQRLNGLPVECGGDTIAEGIAVKDVGDTALRIIRALVKEVLVVKEETIERAVAAL
                     IEIEKTVAEGAGAAALAALLEHPARFAGRRVGLPISGGNIDSRVLASVLMRGLVRDAR
                     LVRLRVTMPDVSGSLAKVAALIAEAGGNIVEVQHQRVFGTASVKSTEIEFLIETRDRE
                     HTDSLIALLEKNGLRVSMF"
     gene            complement(2785..>3700)
                     /locus_tag="VIG70_03310"
     CDS             complement(2785..>3700)
                     /locus_tag="VIG70_03310"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEY8249608.1"
                     /translation="GHARRDEMYAHHLDREGNRVSYQELVRLAPASVKEQISEKAIAD
                     RVAACERHLDRLARSIADARLDALVVVGDDQREQYFEDNMPAFLVYCGATIVNSVLEL
                     PGAPEYWRRARSVYHEPDTPREYPVARDLALHLVDALMDEFDLSYADRLAKPRGEGHA
                     FGFVHRRLMNSQPVPVVPLVVNTYYPPNQPRPRRCFRLGSAIRQAVERFRGNERIGII
                     ASGGLSHFTIDEALDRRVLEACRSGDEEALAQIAPARLNSGNSEIRNWIVMAGAAAGL
                     RPQWQEYVPLYRTPAGTGCGMAFAAWSR"
BASE COUNT          564 a         1266 c         1273 g          597 t
ORIGIN      
        1 cgaagcgata gcggccgtcg gcccgcttga ggaacatcgc cgaggggcta tgggaggtga
       61 acgcgtgcag ccgttcctcg ctctcgcgca gccgctggtc ggcggcgcgc cgctcggtga
      121 tgtcgtcgac ctgcgcgacc accctggtca ccgtgccctc gcggtcgacg atgcgcgtga
      181 cgctggcgcg cacccagagc acggcgccgt ccttgcgcac gaagcgcttc tcgagctcga
      241 gccggtcggc ctcgccggtg aggatcgcct ggaaggcgcg ccagctttcg gcgatgtcct
      301 cggggtgggt caggtcgaag atgctcttct cgcgcagctc ggcctcggcg tagcccacca
      361 gccgcaggaa gctcgcgttg gcgccgagat agcgcccggt gatgtccgcg acggtgatgc
      421 cgttggcggc gctgtcgaaa gtcgccgcga acagctgctc gcgctcgcgc tcgaagtggc
      481 gcgcggtcat gtcgcgcgtg accttggcgt agccgccgag ggcgccgccg gcgccacgca
      541 gggcggtgat cgtcacctcg gcccagaagc gggtgccgtc cttgcgcacg cgccagcctt
      601 cgccctggaa gcggccctcg cgcgcggcgt ggccgagcgc ctgctcgggc ttgccggcgg
      661 cccggtcctc cggggtgtag aacagggaga aatgccggcc ggtcacctcc ccggcgcggt
      721 agcctttgat gcgctcggcg ccggcgttcc agctcgccac ccggcccgag acgtcgagca
      781 ggtagatcgc acaatccgta acgctggaca cgagcaggcg aaactgctcg tcggtggggt
      841 cggcgccgcg cgcgacgtgg tcagcgattc gggtgagttc catgccggga ggagcatcat
      901 aatcccgaaa atgagcggga gaatcctggt ggtcgaggac gaggaggtga tgcgcgaatt
      961 gctgcgcctg cacctgtcct cggccggcta tgccgtggag ctcgccgagg atgccatcgg
     1021 cgcgggctac gcggtgctca agaccctgcc cgacctgatc atctgcgacg tcgagatgcc
     1081 gcacatggac ggcttccagt tcatcgccgc gctgcgcgcc gacaagacgc tgccccggct
     1141 tccggtgatc ttcctgacct ccgtggtcga aggcgagggc gccgcgcgcg tcgagctcgg
     1201 cgccgccgac tacctgttca agccggtcag gctcgaggag ctgctggcgg ccgtgtcgcg
     1261 ccagcttcgg ccgcagcgct agggatttct tcccagttga tctccaccgt cgtgctgagg
     1321 tagcgcgcga ggccgatgat caatcgcagc cgatccttgg ccagccgcaa cggcagctcg
     1381 tccagggtga gctgggcctg ctcctggatc tgggcgagtt tggtgagaat ttcctgcttg
     1441 ctttgcatgt tcttggatcc tttttactcc ggaaccgcgg cgccgcctat tccggcgcgc
     1501 ccgaatctcg tgtagcgctg ctacgacagc aagtaaactt tgccatgctc gagacttcca
     1561 aagccgtcgt ctccgaagcc gccgtctcta tcgccgatat tcgcgccgcg gccgcgcgca
     1621 tcgccggggc gatcgagcac accccctgcg tgcacagccg cacgctgtcg aagctcaccg
     1681 gcgccgaggt gttcctcaag ttcgagaacc agcagttcac cgcctccttc aaggagcgcg
     1741 gcgcgctgaa caagctcctg tcgctcggcg cggccgagcg cagccgcggg gtgatcgcga
     1801 tgagcgcggg caaccacgcc caggcggtcg cctaccacgc cgcgcgcctc ggcatcccgg
     1861 cgctgatcgt gatgccgaaa ggctcgccga acaccaagat ccgcaacacg cagatccacg
     1921 gcgcgaccgt ggtgctcgag ggcgagaacc tgttcgaggc cggcaagcac gcgcgcgccc
     1981 tcgccgcacg cgacaacctg gtcttcgtgc atccctacga ggacccgctg atcatcgccg
     2041 gccagggcac cgtcggcctg gaaatgctcg aggcggtgcc ggacctcgaa gtgctggtgg
     2101 tgccggtcgg cggcggcgga ttgatctccg gagtcgccac cgcggcaaag gctctgcgca
     2161 gctcgatcga ggtctacggc gtggagagca agaactatcc ctcgatgcac cagcgcctga
     2221 acggccttcc ggtcgagtgc ggcggcgaca ccatcgccga aggcatcgcg gtgaaggacg
     2281 tcggcgacac cgcgctcagg atcatccggg ccctggtgaa ggaagtcctg gtcgtcaagg
     2341 aagaaaccat cgagcgcgcg gtggcggcgc tgatcgagat cgagaagacc gttgccgagg
     2401 gcgcgggcgc cgcggcgctc gccgcgctgc tcgagcaccc cgcgcgcttc gccggccggc
     2461 gcgtcgggct gccgatctcg ggcggcaaca tcgacagccg cgtgctcgct tcggtgctga
     2521 tgcgcggcct ggtgcgcgac gcgcgcctgg tgcgcctgcg ggtcaccatg cccgacgtct
     2581 cgggcagcct cgccaaggtc gccgcgctga tcgcggaagc cggcggcaac atcgtcgagg
     2641 tgcagcacca gcgcgtgttc ggcaccgcgt cggtcaagtc gaccgagatc gagttcctga
     2701 tcgagacgcg cgaccgcgag cacaccgact cgctgatcgc gctgctcgag aagaacgggc
     2761 tcagggtctc gatgttctga cgcttcagcg gctccaggcg gcgaacgcca tgccgcagcc
     2821 ggttcccgcc ggcgtgcggt agagcggcac atattcctgc cattgcggcc gcagccccgc
     2881 ggcggcgccc gccatcacga tccagttgcg gatttccgaa ttgccggaat tgagcctggc
     2941 gggcgcgatc tgcgccagtg cttcttcatc gccggacctg caggcttcga gcacccggcg
     3001 atcgagcgcc tcgtcgatcg tgaagtggct caggccgccc gaagcgatga ttccgattct
     3061 ctcatttcct cgaaaacgct ccacggcttg acggattgcg ctgccgaggc gaaagcagcg
     3121 ccgcgggcgc ggctggttcg gcgggtagta ggtgttgacc accaggggca ccaccggcac
     3181 cggctgggaa ttcatcagcc tgcggtgcac gaagccgaac gcgtgaccct cgccgcgcgg
     3241 ctttgccagc cggtcggcat aggacaggtc gaactcgtcc atcagcgcgt ccaccaggtg
     3301 cagcgcgaga tcgcgggcca ccgggtattc gcgcggcgtg tcgggctcgt gatagaccga
     3361 gcgcgcccgc ctccagtact ccggcgcccc cggcagctcg agcaccgagt tgacgatggt
     3421 cgcgccgcaa tagacgagga acgcgggcat gttgtcctcg aagtactgct cgcgctggtc
     3481 gtcgcccacg acgaccagcg cgtcgagcct ggcgtcggcg atcgagcgcg ccaggcggtc
     3541 gaggtgccgc tcgcaggcgg cgacacggtc cgcgatcgct ttttcagaaa tctgctcttt
     3601 caccgaagcg ggagcgaggc gcaccagctc ctgatagctc acgcggttcc cctcgcggtc
     3661 gaggtggtgc gcatacatct cgtcgcggcg cgcgtggccc
//
LOCUS       DATBSZ010000051         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1577630, whole genome shotgun sequence.
ACCESSION   DATBSZ010000051 DATBSZ010000000
VERSION     DATBSZ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577630"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VIG70_03315"
     CDS             <1..702
                     /locus_tag="VIG70_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEY8249609.1"
                     /translation="LEARRGKPSAAGALDYNFHVENDRVRVVPRKRGAPLDKLVSEMM
                     ILANSAWGELLAERDVAAIYRVQSTGKVRFSVHPEPHEGLGVASYAWMSSPLRRYVDL
                     VNQWQLAAALAGRRPPYARNSDALLAALRAFEVTAARYDEHQRGMETYWSLRWLLQEG
                     VRTLDAVVLRENMVRVEGLPLVTRVSAAPTLEPGALVRVEVSGVDLIERSLGAVYRET
                     LGSVRNSSEEAGQKA"
     gene            734..1603
                     /locus_tag="VIG70_03320"
     CDS             734..1603
                     /locus_tag="VIG70_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY8249610.1"
                     /translation="MHFIKRGHDRVLGAALGLSLALHIALLSVHFRSPDGLRWKQESA
                     PLEVVLVNAKTKDRPAKADVLAQSRLNRGGTVEENRRAKTPLPVTEPREPGKDLADAQ
                     RRQRSLEAKQQELLAQMSATQSRAPAPAPRQTGVDEPGAHPSGRDMADLALAAMRLQA
                     QIDRKIDEYQKRPRKTFIGANAAEYRFAQYEEDWRVKVERVGTLNYPAEARGKIYGNL
                     RLTVTIRPDGNVESIELDRSSGLKVLDAAAFKIVRMATPFAAFPPDIRRDTDLLVITR
                     TWFFGQGDKIWTE"
BASE COUNT          297 a          556 c          583 g          239 t
ORIGIN      
        1 ctggaagcac gacgcggcaa gccgagcgcc gccggggcgc tcgattacaa cttccacgtc
       61 gagaacgacc gcgtgcgcgt cgtgccgcgc aagcgcggcg cgccgctcga caagctggtt
      121 tccgagatga tgatcctcgc caactcggcc tggggggagc tccttgccga gcgcgacgtc
      181 gccgccatct atcgcgtgca atccaccggg aaggtgcgtt tcagcgtgca tcccgagccg
      241 cacgagggcc tgggcgtggc gtcctacgcc tggatgagct cgccgctgcg gcgctacgtc
      301 gacctcgtca accagtggca gcttgccgcc gcgctcgccg gccgccggcc gccctacgcg
      361 aggaattcgg acgcgcttct ggcggcgttg cgcgcattcg aggtaacagc cgcgcgctac
      421 gatgagcacc agcgcgggat ggagacctac tggagcctgc gctggctgct gcaggagggt
      481 gtcaggacgc tcgacgcagt ggtgctgcgc gagaatatgg tgcgggtaga gggtttgccg
      541 ctcgtgacgc gggtgagcgc cgcgccgacg ctcgagcccg gtgcgctcgt gagggtggag
      601 gtgagcggcg tcgacctgat cgagcgctca ctcggtgcgg tgtaccgcga aaccctcggc
      661 agcgtgcgaa attccagcga ggaggctggc caaaaagcct aaaatatgaa aggcttgtcg
      721 tactaaggtg agaatgcact tcatcaagag ggggcatgac cgcgtgctcg gcgcggcgct
      781 cgggctgtcg ctcgcgctgc acatcgcgct tctgtcggtg cacttccggt cgccagacgg
      841 gctgcggtgg aagcaggaaa gcgcgcccct ggaagtggtc ctcgtcaacg cgaaaaccaa
      901 ggaccgaccc gcgaaagcgg acgtgctggc ccaatcgcgc ctgaaccgcg ggggtacggt
      961 cgaagagaac cgccgtgcga agacgccgct tccggtgacc gagccgcgcg agcctggcaa
     1021 ggatctcgcg gacgcgcagc gccgccagcg cagcctcgag gcgaaacagc aggagctgct
     1081 cgcccagatg agcgcgacgc agagccgcgc gcccgcaccc gcgccgcgcc agaccggcgt
     1141 ggacgagccg ggcgcgcacc cgagcggccg cgacatggcc gacctcgcgc tcgctgcgat
     1201 gcgcctgcag gcgcagatcg accggaagat cgacgaatac cagaagcgcc cgcgcaagac
     1261 gttcatcggc gcgaacgccg ccgaataccg ctttgcgcag tacgaggaag actggcgcgt
     1321 taaggtcgag cgcgtcggca cgctcaacta cccggccgag gcgcgcggca agatctacgg
     1381 caacctgcgg ctcacggtca ccatccggcc cgacggcaac gtggagtcga tcgagctcga
     1441 ccgctcctcc gggctcaagg tgctcgatgc ggcggcgttc aagatcgtgc gcatggcgac
     1501 gccgttcgcc gcgttcccgc cggacatccg gcgcgacacc gatctcttgg tcattacgcg
     1561 aacttggttc ttcggccagg gcgacaagat ctggactgag tagcgagatg agcgaccggt
     1621 acgcggtcat cggcaacccg gtcgcgcatt ccaagtcccc ctggatccac gcgga
//
LOCUS       DATBSZ010000052        14107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1918977, whole genome shotgun sequence.
ACCESSION   DATBSZ010000052 DATBSZ010000000
VERSION     DATBSZ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918977"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VIG70_03325"
     CDS             complement(<1..218)
                     /locus_tag="VIG70_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY8249611.1"
                     /translation="MKIAAVEAVPLSIPFMYGAEGWQLGSGGWKALDFCLVRVETDTG
                     LTGWGEAFSYSCRRAVAAAVRDMIAPLA"
     gene            complement(220..483)
                     /locus_tag="VIG70_03330"
     CDS             complement(220..483)
                     /locus_tag="VIG70_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEY8249612.1"
                     /translation="MFRLHLWAFVLVNLGLNAANWFTGGDWWAFWPLVVWGGVLVAHY
                     LIYKSKRVDERWVEERTDDVRSKSYDRAHMDSIEARHSDPEKK"
     gene            complement(487..909)
                     /locus_tag="VIG70_03335"
     CDS             complement(487..909)
                     /locus_tag="VIG70_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249613.1"
                     /translation="MDLDWEKLVRRYVWHDERTPYFTRVANLTRRQAHYELLAYAIFM
                     GVAAGVIAVAAPSNWVSLYAFSVCCAALLLGMTRHPWAAIWCALAPLGALAGFAVEGF
                     HPRLETAEKALLVAAALAGLAYSRRLVAVARAWPQLPE"
     gene            complement(910..1671)
                     /locus_tag="VIG70_03340"
     CDS             complement(910..1671)
                     /locus_tag="VIG70_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY8249614.1"
                     /translation="MSERLPLTVRVAQRLRGAVDFIGRWAAWLSVPVVVITCIDVIGR
                     KLAYIDDETGRVHSLQLWLSGNIGRFFDSTVLQELEWHFHAALFALVLGYGMVYNTHV
                     RIDLIRDHLDFRRKAWLEFVGLTFFMLPYCALVIWFALDYAHSSYLVHEQSSSTIGLT
                     HRWIIKSVLVFGLIVALVSGIAAWLQVATILWGPRELRFPLMTLDWPEEDVKVEGKTR
                     VRLEDDYGLRLSGDPGNAKPADSPEPRRRATQAAE"
     gene            complement(1664..3160)
                     /locus_tag="VIG70_03345"
     CDS             complement(1664..3160)
                     /locus_tag="VIG70_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY8249615.1"
                     /translation="MEALQRLGSRLANRYALAVALIVLLAIGYVYMTGLSPAARTGLL
                     ERGREFLPLVMFLTLGVLLFSGYPVAFILGGIALLFGLLGLLLGSFKMVHFFNFVPRI
                     WGQAAENLVLVAIPTFIFMGVMMERSGIAQDLLYCVQVLLKRVPGALALGVTIMGTIL
                     AAMTGIIGASVTMMTALALPTMMKQGYSHALACGTIAAAGTLGILIPPSIMLIIMADL
                     MSVSVGHLFSAAIVPGLTLATLYLLYIYTAAKLKPSLAPPLPPDLLKVPKGQMARLLW
                     RAFFPPVFLIGCIKLSILLGWATPSEAGAVGAAGTIVLAALWGRLNFKTLDAVCQSSA
                     RTIAMVFFIIISATCFAFVYRQLGGDEIVEHLIREAGLGSWGFMFLVMAIVFFLGFFL
                     DWIEITLIVLPVFTPLVATLDFGDHVAKADIVYWFLICLAVNLQTSFLTPPFGFALFY
                     IKGIAPKEVKIQSIYKGIIPFVILQLIGLILVLSFPNLALWLMKFGYE"
     gene            complement(3208..4296)
                     /locus_tag="VIG70_03350"
     CDS             complement(3208..4296)
                     /locus_tag="VIG70_03350"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8249616.1"
                     /translation="MTRTSQLGAAAAALAFVAVSTASFAQQKVSWRMQSAFGSSLTHL
                     GTSGVRFVKDVEEMTDGNFSIRFHEPGALVPALECFDAAAKGSVDACWTTPGYHTGKY
                     PALAFFTTVPFGPSYGEFMAWKIFGNGNKLRQEIYDKHDLMAIDCFAIGPETSGWFRR
                     EIKSTDQLKGLKMRFFGLGAQVMQKLGVSTQLLAGGDIFPALERGVIDATEFSMPAMD
                     IKLGFHQVAKFNYFPGWHQQVSVSELLMPKKSFTSLSKANKRILEVAAGYQVHYTYAE
                     SEASQFAVMAEMRTKHKVQIKRWTDKDLAVFEKAWMEVLKEESAKDPLFKKIADDFLA
                     FRAQYAIWGDSQAMKPTYLPKAAAGTTK"
     gene            complement(4342..5688)
                     /locus_tag="VIG70_03355"
     CDS             complement(4342..5688)
                     /locus_tag="VIG70_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEY8249617.1"
                     /translation="MSANPSDFSPPLLRIQQSAPPPLAGWMLRLLLGLITCLLLWAAF
                     GKLDIVAVADGKLVPSTYLKIVQPAEQGIVKEILVKEGEAVVAGQVLIRMDSVLAGAD
                     VKAIRADHDNKRLALRRIDAQLAGRRFIREKGDPAELALQVEAQYAANVRAYENALAQ
                     ERSLLEKARHDLAAAEGTKAKLEQVLPHYVEQERAFEKLTKDGFAGRILYTDKRRERI
                     EKEQDLRTQEFTIGASRALIEQSEKKIAQIGADYRRQLQTERVEIAAQLEKLTQELAK
                     LSHRQELLELRAPTAGVVKDLATHTAGTVAAPGTILMTLVPEGDKLVAEVWVSNQDVG
                     FVRPGQDAKLKLVTFQFQKYGMLEGRVAHVNADATEAPSPNTRSDSLSRDRPMGPLAF
                     RALLDLNRQELLADGQRYPLQPGMQVAGEIHLGTRTVMEYLLSPVQKAFHEAARER"
     gene            complement(5690..7414)
                     /locus_tag="VIG70_03360"
     CDS             complement(5690..7414)
                     /locus_tag="VIG70_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEY8249618.1"
                     /translation="MFVRHESAKELSPDEGATPASSFGFGWFWRELLKHKRVWRDVLA
                     ASLFIQLVGLATPLFTQVVIDKVVVHHTTNTLVVIALGLAMFLAFNAGMSWLRQYFVL
                     HTGNRVDAVLGSEVFRHVLRLPLPYFEHRATGVLIARVRAVETVREFMAGAAVSLILD
                     FPFLLIFVAVMFAYSWQLTLIALAVLTVIALLSVLVTPLLRSRLNRQFLLGARNQAFL
                     TEYVAGIETVKALQMEPRLEARYDEMLAAYLASGFDTRQVSNSYNVAANALEQLMTLA
                     ILCLGALLVMRNEGFTIGMLVAFQMFAARMSQPMLRLAGLWQEFQQASIAVKRLGDIM
                     DAPAEPYALVASRTPGGRAEIAIAGLAFRYSPEHPYLYRNLSLALKPGRLTVLTGPSG
                     SGKSTLAKLLLGFYPPEDGRITIDGRDIRHFSANELRQYFGVVPQETVLFSGTIYENL
                     LAANPNAAFVDGVNACKIAEIHDFIEKLPKGYNTEIGEHGVGLSGGQKQRLAIARAVL
                     KRPRVLIFDEATSSLDQATAEKFAQTVNKLKGAATILFVAHQLPKGLAVDEVFSLAAE
                     KAVQMRVY"
     gene            7418..7888
                     /locus_tag="VIG70_03365"
     CDS             7418..7888
                     /locus_tag="VIG70_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249619.1"
                     /translation="MRLEKLVSAGERRLAARFEVQEPPAVGLDDEGRPRRLAFQERDA
                     RKVRFCERALAGAEAERLNRFEELAQRVRSRETLQEQRRVERDAAELAQAADQPREVL
                     PCQLLVQAPAPSGILTQPLKELLSPGRILAGCAGETYRQKPRVSLSAETPRQAA"
     gene            7915..7990
                     /locus_tag="VIG70_03370"
     tRNA            7915..7990
                     /locus_tag="VIG70_03370"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7949..7951,aa:Cys,seq:gca)
     gene            8171..9826
                     /locus_tag="VIG70_03375"
     CDS             8171..9826
                     /locus_tag="VIG70_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8249620.1"
                     /translation="MPPAHALPPDTQVLIVGGGPFGLMLANELGRRGIGVALVDQNPG
                     TTQNAAANATQARTMEHFRRLGFADEVRALGLPPDFPTDVAYFTRFARHELARFSLPS
                     SRDARQLVTTLSGSWSAAELPHRCNQKFIEPVLRRHAEALPGVSVNYGHRMTRFREFD
                     DHVEADVESTGSKHTIKARYLIGADGPRSGVRQALGIRYGGETGVTRPFVGGRMYAVH
                     ARIPEFYREVPHRAAWMNVAFNPERRCFMPAVDGHGEFAFHTQLRPHENEDQVTEEEA
                     VAMVQETIGLPLRVEVLARAPWTAGHSLVAEEFSRKRIFIGGDAAHLFTPTGGLGYNT
                     AIEDAVNLGWKLAAVLRGIASENLLESYEAERRPLALRNTAYAKGFADSLGNPVPAPE
                     IEHDTPAGDAARRRAGDYYAAHGRAEFNIPGITFGGRYDGSPAIISDGTTPPPDRANE
                     YVPTACPGGRPPHLWLADGRSLYDCFGFEWTLLRLGAGGGAGFIESARQRSIDLKVVD
                     LKIPEGRDLYAADLALIRPDHIVAWRGNSDSSADAVLHCLTGR"
     gene            complement(9967..11262)
                     /locus_tag="VIG70_03380"
     CDS             complement(9967..11262)
                     /locus_tag="VIG70_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249621.1"
                     /translation="MIEKLNSDCFCISLEPDALRRFLDAHSPGLSALIAQRCPNLFAA
                     LPVFVSRRHVDAMAAVVAAVEEAIKLPGYREAAFAHAPAIARHDPGARGAFLGYDFHL
                     GAGGPRLIEINTNAGGALLNAVLGQAQRACCAEVAALVSGPLRPEALERAFFDMFAAE
                     WRHAGRTGLPRRIAIVDRSPEQQFLYPEFVLFERLFKRFDAEAVIRDPAELVFAGGEL
                     RDGRGRIDLVYNRLTDFSLQDASHDALRRAYLDGAAVVTPHPRAHALYADKRNLALLT
                     DAARLRAWGVRPETLEALVRGIARTELVTEAAAPRLWSSRRKLFFKPAGGYGSKAAYR
                     GDKLTRRVWEEILKGKYVAQELVAPSERRIAADATLKVDVRNYVYSGVVQQLAARLYQ
                     GQTTNLRTAGGGFAPVLTTGTDEGRICGTPQQVPVEAET"
     gene            complement(11259..12314)
                     /gene="arsB"
                     /locus_tag="VIG70_03385"
     CDS             complement(11259..12314)
                     /gene="arsB"
                     /locus_tag="VIG70_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEY8249622.1"
                     /translation="MSVQAGTAPVLGTFERWLSIWVLLCIAAGVALGQLLPGLFQALG
                     RLELARVNLPVGMLIWVMIIPMLLRIDFGAMAQVRRHWRGIGVTLAVNWLVKPFSMAF
                     LAWLFLRQLFAPLLPAGEIDAYIAGLILLAAAPCTAMVFVWSRLTQGDPYFTLSQVAL
                     NDAIMIVAFAPIVALLLGLSSIVVPWDTLLVSVLLYIAIPVAIAQVWRAALLRGGEAR
                     LQRSLEAIQPYSTAALLATIVLLFAFQGRQILSQPLIIAILAVPILIQVYLNSGLAYL
                     LNRRLKVAHAVAGPSALIGASNFFELAVAAAITLFGFDSGAALATVVGVLVEVPVMLS
                     VVGIVNATRGWYERGAA"
     gene            complement(12311..12820)
                     /locus_tag="VIG70_03390"
     CDS             complement(12311..12820)
                     /locus_tag="VIG70_03390"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY8249623.1"
                     /translation="MKSYNVLFLCTGNSARSILAEAYLNLAGRGRFTAYSAGSHPAGK
                     VNPFALELLEKNRLPTAGLRSKNWDEYAKSGAPRLDFVFTVCDNAAGEVCPVWPGQPI
                     SAHWGIADPAAVLGSEQDKRRAFLRAFTELSTRINLFLSLPLDKLDRLVLQKKLDEIG
                     RSPAAEKGL"
     gene            complement(12860..13309)
                     /locus_tag="VIG70_03395"
     CDS             complement(12860..13309)
                     /locus_tag="VIG70_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEY8249624.1"
                     /translation="MKRLHVHVAVHDLQQSIRFYSALFAAEPAVRKDDYAKWMLDDPR
                     VNFAISTRSTKAGLDHLGIQAENGAELEDLGSRLAQADVAITAQKGASCCYAKSDKYW
                     TIDPQGIAWESFHTLDAVPMYGQDTRSRAGEAKDAGKAACCGPAMAK"
     gene            complement(13275..13643)
                     /locus_tag="VIG70_03400"
     CDS             complement(13275..13643)
                     /locus_tag="VIG70_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011548584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8249625.1"
                     /translation="METMQAVQALSALAQESRLAIFRLLVQAGPAGLAAGAIGEKLDL
                     PPATLSFHLAGLTRSGLAQSRQEGRFVIYSASFESMNALVAFLTENCCGGATCAPAAC
                     QPETTKGTESETPTRTRRRA"
     gene            complement(13703..>14107)
                     /locus_tag="VIG70_03405"
     CDS             complement(13703..>14107)
                     /locus_tag="VIG70_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8249626.1"
                     /translation="QVGARVLEFWLMRHVHPIVSARIATLAHPIGAAGLIAAGSPAAT
                     AFAVVHGAGNGVMTISIGTLPLALFGAAGYGLRQGFLMAPARLLSASAPFLFDLLLAR
                     YGTASLAVTAGLGVAAFLVLTVIPVKRASSNQ"
BASE COUNT         2466 a         4700 c         4744 g         2197 t
ORIGIN      
        1 atggcgaggg gcgcgatcat gtcccggact gcggcagcga cggcccggcg gcacgagtag
       61 gaaaatgcct cgccccagcc ggtgaggccg gtgtcggtct cgacgcgcac caggcagaag
      121 tcgagcgcct tccagccgcc ggagccgagc tgccagcctt cagcgccgta catgaacggg
      181 atcgagagcg gcaccgcctc gacggcggca atcttcaagc tattttttct ccggatcgga
      241 gtgacgcgcc tcgatgctgt ccatgtgcgc ccggtcgtag ctcttggagc gcacgtcgtc
      301 ggtgcgctcc tcgacccagc gctcgtcgac gcgcttcgac ttgtaaatca gatagtgcgc
      361 cacgaggacg cccccccaga cgacaagcgg ccagaaagcc caccagtcgc cgccggtgaa
      421 ccagttggcg gcgttcaggc caaggttgac caggacgaaa gcccagaggt gcaggcggaa
      481 cacggcttat tccggcagct gcggccaggc gcgcgcgacc gcgacgaggc ggcgcgagta
      541 ggcgagcccg gcgagcgccg cggcgaccag cagcgccttc tcggcggttt cgagccgggg
      601 atggaagccc tcgacggcga atcccgcgag cgcgccaagg ggcgcaagcg cgcaccagat
      661 cgccgcccac gggtggcgcg tcatccccag gagcagcgcc gcgcagcaga ccgagaaggc
      721 atagagcgaa acccagttcg agggcgcggc gacggcgatc acgcccgcag ccacacccat
      781 gaagatcgcg taggcgagaa gctcgtagtg cgcctggcgg cgcgtcaggt tcgcgacgcg
      841 cgtgaagtac ggggtgcgct cgtcgtgcca gacgtagcgc ctgaccagct tctcccagtc
      901 gaggtccatc tactcggcgg cctgggtggc ccggcggcgg ggctcggggc tgtctgccgg
      961 cttcgcgttg ccggggtcgc ccgagaggcg caggccgtag tcgtcctcca ggcgcacgcg
     1021 cgtcttgcct tcgaccttga catcctcctc cggccagtcg agcgtcatga gcgggaagcg
     1081 cagctcgcgc ggcccccaca ggatcgtcgc cacctgcagc caggcggcga tgccggaaac
     1141 gagcgcgacg atcaggccga acaccagaac gctcttgatg atccagcggt gcgtcagccc
     1201 tatggtggag gacgactgct cgtgcaccaa gtacgaggag tgggcgtagt cgagcgcgaa
     1261 ccagatcacc agcgcgcagt acggcagcat gaagaaggtg aggccgacga actcgagcca
     1321 tgccttgcgc cggaagtcga ggtggtcgcg gatcaggtcg atgcgcacgt gcgtgttgta
     1381 gaccatgccg tagcccagca ccagcgcgaa cagcgcggca tggaagtgcc actccagctc
     1441 ctgcagcacc gtcgagtcga agaagcgccc gatgttgccg gagagccaga gctgcaggct
     1501 gtggaccctg ccggtctcgt cgtcgatata cgcgagcttg cgtccgataa cgtcgatgca
     1561 ggtgatcacc acgaccggca cggagagcca ggccgcccag cgcccgatga agtcgaccgc
     1621 gccgcgcagc cgctgggcga cgcgcaccgt cagcggcagc cgctcactca taaccgaact
     1681 tcatcagcca cagcgcgaga ttcgggaagg agagcaccag gatcaggccg atgagctgca
     1741 ggatcacgaa gggaatgatg cccttgtaga tcgactggat cttgacttcc ttcggcgcga
     1801 tgcccttgat gtagaagagg gcgaagccga acggcggcgt gaggaacgag gtctgcaggt
     1861 tgaccgcgag gcagatcaga aaccagtaga cgatgtctgc cttcgccacg tggtcgccga
     1921 agtcgagcgt ggcgacaagc ggcgtgaaca ccggaagcac gatcagcgtg atctcgatcc
     1981 agtcgaggaa gaagccgagg aagaacacga tcgccatcac caggaacatg aagccccagc
     2041 tccccaggcc ggcctccctg atcaggtgct cgacgatctc gtcgccgccg agctggcggt
     2101 agacgaaggc gaagcaggtg gcgctgatga tgatgaagaa caccatcgcg atggtgcgcg
     2161 ccgagctctg gcacaccgcg tccagggtct tgaagttcag gcggccccac agcgcggcga
     2221 gcacgatcgt cccggccgcg ccgaccgcgc cggcctcgct cggcgtcgcc cagccgagca
     2281 ggatcgagag cttgatgcag ccgatcagga acaccggcgg gaagaacgcg cgccacagca
     2341 ggcgcgccat ctggcccttc ggcaccttga gcaggtcggg cggcagcggc ggcgcgaggg
     2401 aaggcttgag cttcgccgcg gtgtagatgt agaggaggta cagcgtggcc agcgtcagcc
     2461 cgggaacgat cgccgccgag aacaggtggc cgaccgacac cgacatcagg tcggccatga
     2521 tgatcagcat gatggagggc ggaatcagga tgccgagcgt gcccgcggcg gcgatggtgc
     2581 cgcaggcgag cgcgtggctg tagccctgct tcatcatcgt cggcagcgcg agcgcggtca
     2641 tcatcgtcac cgaggcgccg atgatgccgg tcatcgccgc gaggatggtt cccatgatgg
     2701 tgacgccgag cgcgagcgcg cccggcacgc gcttgagcag cacctgcacg cagtagagca
     2761 ggtcctgcgc gatgccgctc ctctccatca tcacgcccat gaagatgaag gtcgggatcg
     2821 ccaccagcac caggttctcc gccgcctggc cccagatgcg cggcacgaaa ttgaagaaat
     2881 gcaccatctt gaaggagccg agcaagaggc cgagcagccc gaagagcagg gcgatgccgc
     2941 cgagaatgaa agccaccggg tagcccgaga acagcagcac tcccagagta aggaacatca
     3001 ccagcggcag gaactcccgg ccccgctcga gcagcccggt gcgcgccgcg gggctcaggc
     3061 ccgtcatata gacgtagccg atggcgagaa gcacgatcag cgcaaccgcc aatgcgtagc
     3121 ggttcgcgag cctgctgcca agcctttgca gcgcttccat aggccaggac accgggatgc
     3181 gcgcggcgcg catcccggaa tcggctgcta cttcgtcgtc cccgccgcgg ccttcggcag
     3241 gtacgtcggc ttcatcgcct gcgagtcgcc ccagatcgcg tactgggcgc ggaacgcgag
     3301 gaagtcatcc gcgatcttct tgaacagcgg atccttggcg gactcctcct tcagcacctc
     3361 catccaggcc ttctcgaaga cggcgaggtc cttgtccgtc cagcgcttga tctgcacctt
     3421 gtgtttcgtg cgcatctcgg ccatgacggc gaactgcgac gcctcggatt cggcgtaggt
     3481 gtagtgcacc tggtagccgg cggcgacctc gaggatcctc ttgttggcct tggacagcga
     3541 ggtgaaggac ttcttcggca tcagcagctc gctcaccgag acctgctggt gccagccggg
     3601 gaagtagttg aacttcgcca cctggtgaaa gccgagcttg atgtccatcg ccggcatgga
     3661 gaactcggtg gcgtcgatca cgccgcgctc gagcgccggg aaaatgtctc cgcctgcgag
     3721 cagctgcgtg gagacgccga gcttctgcat cacctgcgcg cccaggccga agaagcgcat
     3781 cttcaggcct ttcagctggt cggtcgactt gatctcgcgg cggaaccagc ccgaggtctc
     3841 gggcccgatc gcgaagcagt cgatcgccat caggtcgtgc ttgtcgtaga tctcctggcg
     3901 cagcttgttg ccattgccga agatcttcca ggccatgaac tcgccgtagc tcggtccgaa
     3961 cggcaccgtg gtgaagaacg cgagcgccgg gtacttgccg gtgtggtagc ccggcgtggt
     4021 ccagcacgcg tcgaccgagc ccttggccgc ggcgtcgaag cactcgagtg ccggcaccag
     4081 cgcgccgggc tcgtggaacc ggatgctgaa gttgccgtcg gtcatctcct ccacgtcctt
     4141 cacaaagcgc acgccggagg tgccgaggtg cgtgagcgag ctgccgaacg cgctctgcat
     4201 ccgccagctc accttctgct gggcgaagga cgcggtggaa actgcgacga aggcgagcgc
     4261 cgctgcagcg gcgcccaact gggacgttct ggtcatatgc cctcctcctc taaggttggc
     4321 gcgaccatga taacgccaaa atcagcgttc gcgcgccgcc tcgtggaagg ccttctgcac
     4381 cggcgagagc aggtactcca tgacggtgcg cgtgccgagg tggatctcgc ccgccacctg
     4441 catgccgggc tgcagcggat agcgctgccc gtcggccagc agctcttgtc tgttcagatc
     4501 gagcaacgcc cggaaagcga gcgggcccat cggccggtcg cggctgagcg agtcggagcg
     4561 cgtgttcggc gagggcgcct cggtcgcatc ggcgttcacg tgcgcgaccc ggccttcgag
     4621 catgccgtac ttctggaact ggaaggtgac gagcttgagc ttcgcgtcct gcccgggacg
     4681 cacgaagccg acgtcctggt tcgataccca gacctcggca acgagcttgt cgccttcggg
     4741 caccagcgtc atgaggatcg tccccggcgc tgccacggtg cccgcggtgt gcgtggcgag
     4801 gtccttcacc accccggccg tcggcgcgcg cagctcgagc agctcctgcc ggtgggaaag
     4861 cttggcgagc tcctgcgtca gcttctccag ctgggcggca atctcgacac gctcggtctg
     4921 aagctgccgg cggtagtcgg cgccgatctg cgcgatcttc ttctccgact gctcgatcaa
     4981 cgcgcggctc gcgccgatgg tgaattcctg ggtgcgcaga tcctgctcct tctcgatgcg
     5041 ctcgcgccgc ttgtcggtgt acaggatgcg gccggcgaag ccgtccttgg tcagcttctc
     5101 gaacgcgcgc tcctgctcga cgtagtgcgg caggacctgc tcgagcttgg ccttggtccc
     5161 ctcggccgcg gcgaggtcgt ggcgcgcctt ctcgaggagc gagcgctcct gcgccagcgc
     5221 gttctcgtag gcgcgcacgt tggccgcgta ttgcgcctcc acctgcagcg cgagctcggc
     5281 cgggtcgccc ttctcccgga taaacctccg tccggcgagc tgagcgtcga tccggcgcag
     5341 cgccaggcgc ttgttgtcgt ggtcagccct aatggctttc acgtcggcgc cggcgagcac
     5401 cgagtccatc ctgatcagca cttgcccggc aacgaccgct tcgccttcct tcaccaggat
     5461 ttccttcacg atgccctgct ccgcgggctg cacgatcttg aggtacgtgg agggcaccag
     5521 cttgccgtcg gcgacggcga cgatatcgag cttgccgaat gccgcccata gcaatagaca
     5581 ggtgatgagg ccgagcaaga gccgcagcat ccagccggcg agcggcggcg gcgcactctg
     5641 ctggatgcgc agcagcggcg gcgagaagtc cgacgggtta gcgctcatgc tagtacaccc
     5701 gcatctgcac ggccttctcg gcggcgaggc tgaagacttc gtcgaccgcg agccccttcg
     5761 gcaactggtg tgcgacgaag aggatcgtcg ccgctccttt cagcttgttg accgtctgcg
     5821 cgaatttctc cgcggtggcc tggtcgaggc tgctcgtcgc ctcgtcgaag atcaggacgc
     5881 gcgggcgctt caggacggcg cgggcgatgg cgaggcgctg cttctgcccg cccgagagac
     5941 cgacgccgtg ctcgccgatc tcggtgttgt agcccttcgg cagcttttcg atgaagtcgt
     6001 ggatctcggc gatcttgcag gcgttcaccc catcgacgaa cgccgcgttc gggttcgccg
     6061 cgagcaggtt ctcgtagatc gtgcccgaga agagcaccgt ctcctgcggc accacgccga
     6121 agtactggcg aagctcgttg gccgagaaat ggcggatgtc gcggccgtcg atggtgatgc
     6181 gcccgtcctc cggcgggtag aagccgagca gcagcttggc gagggtgctc ttgcccgagc
     6241 cggacgggcc ggtgaggacg gtgagcctgc cgggcttcag ggcgaggctg aggttgcggt
     6301 agaggtacgg gtgctcgggc gagtagcgga aggcaagccc ggcgatcgcg atctccgccc
     6361 tgcccccggg cgtgcgcgac gcgacgagcg cgtacggctc ggcgggcgcg tccatgatgt
     6421 cgccgaggcg cttcaccgcg atcgacgcct gctggaactc ctgccacagg ccggcgaggc
     6481 gcagcatcgg ctgcgacatg cgggctgcga acatctggaa ggcgaccagc atgccgatgg
     6541 tgaagccttc gttgcgcatc acgagcagcg cgccgaggca aaggatcgcg agcgtcatga
     6601 gctgctcgag ggcgttcgcc gccacgttgt agctgttcga cacctgccgc gtatcgaacc
     6661 ccgaggcgag gtaggccgcg agcatctcgt cgtagcgcgc ctccagccgc ggctccatct
     6721 gcagcgcctt caccgtctcg atgccggcga cgtattcggt gaggaaggcc tggttgcgcg
     6781 cgccgagcag gaactgcctg ttgagccgag agcgcaacag cggggttacc agcacgctta
     6841 gaagtgctat caccgtcaag acggcgaggg cgatcagcgt cagctgccag ctgtaggcga
     6901 acatcaccgc gacgaagatc agcaggaacg ggaagtcgag gatcagcgac accgccgcac
     6961 ccgccatgaa ttcgcgcacc gtctcgaccg cacgcacgcg ggcgatcagc acgccggtgg
     7021 cgcggtgctc gaagtaaggc aggggcaggc gcaggacgtg gcggaacacc tcgctgccca
     7081 ggaccgcatc gacgcggttc ccggtgtgca gcacgaagta ctgccgcagc cagctcatgc
     7141 cggcgttgaa cgcgaggaac atggcgaggc cgagggcgat cacgaccagc gtgttcgtgg
     7201 tgtggtgcac caccaccttg tcgatgacca cctgcgtaaa gagcggcgtg gcgagcccga
     7261 caagctggat gaagagcgac gccgcgagca cgtcgcgcca gacgcgcttg tgcttgagga
     7321 gctcgcgcca gaaccagccg aagccgaagc tcgacgccgg cgtcgcgcct tcgtccggcg
     7381 aaagctcctt cgcgctctcg tgacgcacga acacggcatg cggctcgaaa agctcgtatc
     7441 cgccggtgag cggcgtctgg ctgcccgctt cgaagtacag gagccgccgg ccgtcggcct
     7501 tgacgacgag ggccggccgc ggcgcctcgc ctttcaggaa cgcgacgcac ggaaggttcg
     7561 cttttgcgag cgcgccctcg ccggcgcgga agccgagcga ctgaatcgct tcgaggaact
     7621 ggcgcagcga gtgcggagcc gggaaacgct gcaggagcag cgccgggtcg aacgggacgc
     7681 ggctgagctg gcacaggctg ccgaccagcc acgcgaagtg ctgccctgtc agctccttgt
     7741 tcaggctcct gccccctcgg ggattctgac tcagccgctc aaggaactct tatctccagg
     7801 tcggattctg gccggctgcg cgggcgaaac ctatcggcag aagccccgag tgagcctatc
     7861 ggcagaaacc cctagacagg ctgcttaaca aatcgtcccc agaatcgctt gcgtggcgaa
     7921 gtggcagagt ggggagatgc tacggtctgc aaagccgtcc acgttggttc gattccaacc
     7981 ttcgcctcca tcccctttcc cataaaaagc cctgaggaac tatcgggcgt cgcggagctg
     8041 tcacagccgc gccctatact gaagtatcgt tccgtggggc ttgcccgatc gccgagcgta
     8101 gccccgatag cacgccgggt tcggcatccc cttcggacga ctctcagggc tgcgaaaatg
     8161 gcgcccagcc atgccgccgg cgcacgccct tccacccgat acccaggtac tgatcgtcgg
     8221 cggcggcccg ttcgggctga tgctcgccaa cgagctcggc cgccgcggca tcggcgtcgc
     8281 gctcgtcgac cagaatcccg gcacgacgca gaacgccgcc gccaacgcca cccaggcgcg
     8341 caccatggag cacttccgcc gcctcggatt cgccgacgag gtgcgcgcgc tcgggctacc
     8401 gcccgacttc ccgaccgacg tcgcctactt cacgcgcttc gcgcgccacg agctcgcgcg
     8461 cttctccctc ccctcgtcgc gcgacgcgag gcagctcgtc accacgctct ccggatcgtg
     8521 gagcgcggcg gagctgccgc accgttgcaa ccagaagttc atcgagccgg tgctgcgccg
     8581 gcacgcggag gctcttccgg gcgtgtccgt gaattacggg caccgcatga cccggtttcg
     8641 cgaattcgac gaccacgtgg aagccgacgt cgaatcgact ggaagcaagc acaccatcaa
     8701 agcccgatat ctgatcggcg cagacgggcc gcgcagcggc gtgcgccagg cgctcggcat
     8761 ccgctacggc ggcgagaccg gcgtgacgcg ccccttcgtc ggcgggcgca tgtacgccgt
     8821 ccatgcacgc atcccggagt tctaccgcga ggtgccgcac cgcgcggcat ggatgaacgt
     8881 cgccttcaac ccggagcgcc gctgcttcat gccggccgtg gatggccatg gcgagttcgc
     8941 ttttcacacg cagttgcgtc ctcacgaaaa cgaggatcag gtaacggaag aagaggccgt
     9001 cgcgatggtc caggagacca tcggtctgcc gttgcgcgtc gaggtgctcg cgcgcgcgcc
     9061 ctggacggcg gggcattcgc tggtcgcaga ggaattttcg agaaagagaa tctttatcgg
     9121 gggcgacgca gctcacctct tcaccccgac cggcggcctc ggctacaaca ccgcgatcga
     9181 ggacgcggtg aatctcggct ggaagctcgc tgcggtcttg cgaggtattg cgtcggaaaa
     9241 tcttcttgaa agctacgagg cggaacggcg gccgctcgcg cttcgcaaca ctgcctatgc
     9301 gaagggcttc gccgattcgc tcggcaaccc cgtgccggcg cccgagatcg agcacgacac
     9361 gcccgccggc gacgccgcgc gccgccgcgc cggcgattac tacgccgcgc acggccgcgc
     9421 cgagttcaac attccgggca tcacgttcgg cggccgctac gacggttcgc cggcgatcat
     9481 ctccgacggc accaccccgc cccccgaccg ggcgaacgag tacgtcccga ccgcgtgccc
     9541 gggcgggcgc ccgccgcacc tttggctcgc cgacggccgc tcgctctacg actgcttcgg
     9601 cttcgaatgg accttgctgc gcctcggcgc cggcgggggc gcgggcttca tcgagtcggc
     9661 gcggcagcgc agcatcgatc tgaaggtcgt ggacctgaaa atccccgaag gccgcgacct
     9721 ttatgccgcc gacctagccc tgatccggcc cgaccacatc gtggcctggc ggggcaactc
     9781 ggatagcagc gcggacgccg tattgcactg cctgacaggt aggtaataag gactgaaaag
     9841 tctgtggcgt ttttcctcgt cctcccggcg gcgcgccctt accgtgaacg ctcgttccga
     9901 agggctgccc gacagccgag tgcgcatata gcgcgccggg gtcggcagac ccatcgggcg
     9961 agcctctcaa gtttcggctt cgactggaac ctgctgcggc gtgccgcaga tccggccctc
    10021 atccgtcccg gtggtcagca ccggcgcgaa gccgccgccc gcagtgcgaa ggttggtcgt
    10081 ctgcccctgg tacagccgcg ccgcgagctg ctgcacgacg cccgaataga catagttgcg
    10141 cacgtcgact ttgagcgtgg catccgcggc aatgcgccgc tcgctcggcg caacgagctc
    10201 ctgggccacg tatttccctt tgaggatttc ctcccacacg cgccgcgtga gcttgtcgcc
    10261 gcgatacgcc gccttgctgc cgtaaccgcc ggcgggtttg aagaaaagct tgcgccggct
    10321 cgaccagagg cgcggcgcag ccgcctcggt gacgagttcg gtgcgcgcga tgccgcgcac
    10381 cagcgcctcg agcgtttccg ggcgcacgcc ccaggcccgc agccgcgccg cgtcggtgag
    10441 caaggcgagg ttgcgcttgt cggcgtagag cgcgtgggcg cgcgggtggg gcgttacgac
    10501 agcggcgccg tccaggtagg cccggcgcag cgcatcgtgc gatgcatcct gcagggaaaa
    10561 atccgtcagg cggttgtaga cgaggtcgat ccggccccgg ccgtcgcgca gctcaccgcc
    10621 cgcgaatacg agctctgcgg ggtcgcggat cacggcctcg gcgtcgaagc gcttgaacag
    10681 gcgctcgaac agcacgaact cgggatagag aaactgctgc tccggagagc ggtcgacgat
    10741 cgcgatgcgg cgcggcagac cggtgcggcc ggcgtgccgc cactcggcgg cgaacatgtc
    10801 gaagaatgcg cgctccagcg cctccgggcg cagcgggccg ctgacgagcg ctgcgacctc
    10861 ggcgcagcag gcacgctgcg cctgccccag caccgcgttg agcagcgcgc cgccggcatt
    10921 cgtattgatc tcgatcagcc gcggaccgcc tgcgccgaga tggaaatcgt agccgaggaa
    10981 tgcgccgcgc gcgccggggt cgtgccgcgc gatcgccggc gcatgtgcga acgcggcttc
    11041 acggtacccg ggcagcttga tcgcctcctc caccgccgct acgaccgcgg ccatcgcgtc
    11101 gacgtgacgg cgcgaaacga agacgggaag cgcggcgaaa aggttcgggc agcgctgcgc
    11161 gatcagggcc gatagcccgg gcgagtgcgc gtcgagaaaa cggcgcagcg cgtcgggctc
    11221 gaggctgatg cagaagcagt cgctgttcag cttctcgatc atgccgcgcc ccgctcgtac
    11281 cagccgcgcg tcgcgttcac gatgccgacc accgagagca tcaccggcac ctcgaccagc
    11341 acgcccacga ccgtggcgag cgccgcgccc gagtcgaagc cgaacagcgt gatcgccgcc
    11401 gcgaccgcga gctcgaagaa gttgcttgcg ccgatcagcg ccgaaggccc ggcaacagcg
    11461 tgcgcgacct tgagccggcg gttgaggagg taggcgaggc ccgagttcag gtacacctgg
    11521 atcaggatcg gcacggccag gatggcaatg atgagcggct gggagaggat ctggcggccc
    11581 tggaacgcga agagcagcac gatggtggcg agcagcgccg ccgtcgagta cggctggatc
    11641 gcctcgagcg agcgctgtag ccgcgcctcg ccgccgcgca gcagcgccgc gcgccagacc
    11701 tgtgcgatgg cgaccgggat ggcgatatag agcagcaccg aaaccagcag cgtgtcccac
    11761 ggcaccacga tcgacgagag gccgagcaac agcgccacga tgggggcgaa ggcaacgatc
    11821 atgatcgcgt cgttgagcgc cacctggctg agcgtgaagt acggatcgcc ttgggtgagg
    11881 cggctccata cgaagaccat cgccgtgcag ggcgccgcag caagcaggat caggccggcg
    11941 atgtacgcat cgatctcgcc ggctggcagg agcggcgcga acagctgccg caggaacagc
    12001 caggcgagga acgccatcga gaatggcttc accagccagt tgacggcgag ggtgacgccg
    12061 atgccgcgcc agtgccggcg cacctgcgcc atcgcgccga agtcgatgcg cagcagcatc
    12121 gggatgatca tcacccagat gagcatcccg accggcaggt tgacccgcgc cagttcgagc
    12181 cgcccgagcg cctggaacaa ccccggcaga agctggccga gcgccacgcc cgcagcgatg
    12241 cacagcaaca cccagatcga aagccagcgc tcgaaggtcc cgagcactgg cgcggttccg
    12301 gcctgcacgc tcacagtccc ttctccgccg ccggactccg gcctatctcg tcgagcttct
    12361 tctgcagcac cagccggtcc agcttgtcga gcggtaggct caggaacagg ttgatccgcg
    12421 tcgacagctc ggtaaatgcg cgcaggaacg ctctgcgctt gtcctgctcg ctaccgagca
    12481 ctgccgccgg atcggcgatc ccccagtgcg cgctgatcgg ctggcccggc cagaccgggc
    12541 agacttcgcc cgccgcgttg tcgcagacgg tgaagacgaa atcgagccgc ggcgccccgc
    12601 tcttggcgta ctcgtcccaa ttcttcgagc gcaaccctgc ggtcggcagc cggttcttct
    12661 cgagcagctc gagcgcgaac gggttgacct tgccggcggg atgactaccc gcgctgtagg
    12721 ccgtgaacct gcccctcccc gcaaggttca ggtacgcctc ggcgaggatc gacctcgccg
    12781 agttgccggt gcataggaag aggacgttgt aggacttcat gccggctcgc tgatgaaatc
    12841 gaagctcatg atgttctcgc tatttcgcca tggcggggcc gcagcacgcg gctttgccgg
    12901 catctttcgc ttcgcccgcg cgcgagcggg tgtcctggcc gtacatcggc accgcgtcga
    12961 gtgtatggaa ggactcccag gcgatgccct gcgggtcgat ggtccagtac ttgtcgctct
    13021 tcgcgtagca gcacgacgcg cctttctgcg ccgtgattgc gacgtcggct tgcgcgagac
    13081 ggctgccgag gtcctccagc tcggcgccgt tctcggcctg gatgccgaga tggtcgagcc
    13141 cggccttagt cgagcgcgtc gagatcgcga aattgacgcg cggatcgtcc agcatccact
    13201 tggcatagtc gtccttcctt actgccggct cggcggcgaa cagcgccgag taaaagcgga
    13261 tgctttgctg caggtcatgc acggcgacgt gtacgtgtag gcgtttcact ttcagttcct
    13321 ttcgtggttt cgggttggca cgcggcgggc gcgcacgtag ccccgccgca gcagttctcg
    13381 gtgagaaacg cgaccagtgc gttcatgctt tcgaagctgg cgctgtagat gacaaagcgg
    13441 ccttcctgcc ggctctgcgc gagtcccgag cgcgtgagac ccgccaagtg aaaagacagg
    13501 gtggcgggcg gcagatccaa cttttctccg atcgcgcccg cggcgagccc ggcgggaccc
    13561 gcctgcacca gcagccggaa aatggcgagg cgggactctt gcgctagggc ggagagggct
    13621 tggacagctt gcatcgtttc catgattcca gaattatgga aatgacgccc gttgtcaagc
    13681 cctcggccgc cgataggcta tttcattgat ttgaagaagc tcttttgacc ggaatgaccg
    13741 tcaaaaccag gaaagcggcg acgcccagac cggccgtcac ggccagcgat gccgtcccat
    13801 agcgcgccag cagcaggtcg aagagaaaag gcgcgctcgc cgagagcagc ctggccggcg
    13861 ccatcaggaa tccctgtctc aatccatagc cggcagcgcc gaagagcgcg aggggcagtg
    13921 tgccgatcga gatcgtcatc acaccattgc ccgcgccgtg cacgaccgcg aacgcagtgg
    13981 cggctgggga gccagcggcg atcaatccgg cagcgccgat tggatgggcc agcgtggcga
    14041 tgcgcgcgga aacgatcggg tggacgtggc gcatcagcca gaactcgaga acgcgcgccc
    14101 cgacctg
//
LOCUS       DATBSZ010000053         3811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1577714, whole genome shotgun sequence.
ACCESSION   DATBSZ010000053 DATBSZ010000000
VERSION     DATBSZ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577714"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..387)
                     /locus_tag="VIG70_03410"
     CDS             complement(82..387)
                     /locus_tag="VIG70_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249627.1"
                     /translation="MEDSESTITDDIFSLGVGGLQPERQLLPRGELLARVSALMQSCE
                     GCENVVVVDVTSLDRPDTAGCNWSYTLVLDTAGVPAEVYGLAYAQVIGMARSSWNLE"
     gene            complement(472..1176)
                     /locus_tag="VIG70_03415"
     CDS             complement(472..1176)
                     /locus_tag="VIG70_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249628.1"
                     /translation="MARAPDPNLGLLIAVAQALGELRERFVFVGGCATGLLITDPAAP
                     AARPTRDVDVIVEAGALLEYREIGRALQAKGFSQPLAAGDPPYRWTGRDGSKLDVMPT
                     EEAILGFSNRWYRDAIRTAREIALRERLSIRLVTAPYFLATKYEAFLGRGRGDYLASH
                     DFEDLIAVIDGRPELEAEVRGCEPRLREHLAAVMRTTIADERFTSALGGFVIENGGSP
                     GRASSLFARMQRLAGL"
     gene            complement(1177..1695)
                     /locus_tag="VIG70_03420"
     CDS             complement(1177..1695)
                     /locus_tag="VIG70_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249629.1"
                     /translation="MILKPQDVVVLLKLVALGEAGWTYQSLASELSLSPSEAHAGVRR
                     AVAARLMTDAKSATGRPIRAALKEFLLHGVKYAFPPERGELTRGVPTGYAAPPLNKEI
                     AQGNDPPPVWPHAEGKVRGYALKPLYKSVPLAAGRDPALYELLALVDAIRDGRARERA
                     LAAREIEQRLEG"
     gene            complement(1842..2249)
                     /locus_tag="VIG70_03425"
     CDS             complement(1842..2249)
                     /locus_tag="VIG70_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8249630.1"
                     /translation="MELRIIKTGEQHRRYVEEARRLAKGDPAPQSDDGARLELLAKLI
                     DDYERERFKFRKPDPIDAIVFRMEEQGLRQADIAAIVGGKSRASEILSRKRPLTLSMV
                     RALHEKLGIPSELLIREPAAEYRVRRRGGKRTR"
     gene            complement(2251..2541)
                     /locus_tag="VIG70_03430"
     CDS             complement(2251..2541)
                     /locus_tag="VIG70_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HEY8249631.1"
                     /translation="MKVLDRERLERFCRKHTDARGWIENWLADVESAAWTTPQDIRRR
                     YASASFLPEGVVIFNVKGNAYRLEVTVAYRTGVVVIDWIGTHREYDERNKRR"
     gene            2766..3617
                     /locus_tag="VIG70_03435"
     CDS             2766..3617
                     /locus_tag="VIG70_03435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8249632.1"
                     /translation="MQSATAVASNTQPVNPNKALWEKGDFTRIAETMRASGDALVASL
                     GVAKGMKVLDLGCGDGTTAIPAAQRGADVLGVDIAANLVAAGNRRAQGLGLANVRFQQ
                     GDACDLQGVPDKSFDLLVSIFGAMFAPRPLEVAKAMVRVVRPGGRIVMGNWIPNDPTL
                     VAQILKISSAYTPPPPAGFVSPMTWGVEATVAERFAAAGVPAANVAFARDTFTFDFAG
                     TPAELVGRFRDFYGPTMNAFDAAQKSGRVAELQRELEALFAAQNSSGRADRTTIAATY
                     LRVSVSL"
BASE COUNT          618 a         1345 c         1233 g          615 t
ORIGIN      
        1 gtgcatgttg gggtcagggc tcgaattctg tttttgcgta cgcgaaaacg aattcgggcc
       61 ctgaccccga attttcagcg cttactccag gttccagcta ctccgcgcca tcccgatcac
      121 ctgagcgtag gcgagcccat aaacctccgc cggcacgccc gccgtgtcga gcacgagcgt
      181 gtacgaccag ttgcagccgg cggtatcggg acgatcgagc gacgttacgt cgacgacgac
      241 cacgttctcg catccctcgc agctttgcat caaggcgctc acgcgtgcga ggagctcgcc
      301 gcgcggcaag agctggcgtt ctggctgcag gccgccgacg ccgagggaaa agatgtcgtc
      361 ggtgatcgtc gactcggagt cttccatggg tgcgcgattt tatatcgaag caacccggtg
      421 caggcgtgat cgagctcgtg ctcgggccgc gctggcgcgg aagaactctc cctacaggcc
      481 tgcgaggcgc tgcattcgcg cgaaaaggga ggacgcccgg cccgggctgc cgccgttttc
      541 gatgacgaag ccgccaagcg cggaggtaaa gcgctcgtcg gcgatcgtcg tgcgcatcac
      601 ggcggcgagg tgctcgcgca gccgcggttc gcagccgcgc acctcggctt cgagctccgg
      661 ccggccgtcg atcaccgcga tgagatcctc gaagtcgtgg cttgcgagat agtctccgcg
      721 gccgcgcccg aggaacgcct cgtacttggt cgcgaggaag tacggcgccg tcaccagccg
      781 gatcgagaga cgctcgcgca acgcgatctc gcgcgccgtg cggatcgcgt cgcgatacca
      841 gcggttggaa aaaccgagta tggcttcctc cgtcggcatg acgtcgagct tggagccatc
      901 gcggccagtc cagcgatagg gcggatcccc cgcggcgagc ggctgcgaaa agcccttcgc
      961 ctggagggca cggccgatct cgcgatactc gagcagcgcg ccggcttcga cgatgacgtc
     1021 gacatcgcgg gtcggtcgcg cggcgggggc agcgggatcg gtgatcagca gccccgtggc
     1081 gcagccgccg acgaagacga agcgctcgcg cagctcgccc aacgcctggg cgaccgcgat
     1141 gagcagcccg agattcggat ccggcgcccg cgccattcac ccctccagcc gctgctcgat
     1201 ctcgcgtgcg gccagcgcgc gctcgcgcgc gcggccgtcg cggattgcgt cgaccagcgc
     1261 cagcagctca tagagcgcgg gatcgcgccc ggcggcgagc ggcacggatt tgtaaagcgg
     1321 cttgagcgca tagcctcgaa ccttgccctc ggcgtgcggc cagaccggcg gcgggtcgtt
     1381 gccctgggcg atctccttgt tgagcggcgg tgccgcgtag cctgtcggca cgccgcgcgt
     1441 gagctcgccc cgctcaggcg gaaacgcgta tttcacgccg tgcagaagga actccttcag
     1501 tgcggcgcgg atcggccggc cggtcgcgct tttcgcgtca gtcataagcc gggcggccac
     1561 cgcccggcgc acgccggcat gcgcctccga cgggctgagc gacagctcgc tcgccaagct
     1621 ctgataagtc catcccgcct cgccgagcgc caccagcttg agtaacacca ctacgtcttg
     1681 cggcttaaga atcatacgct tatgctattc gcgaatcgcg aatagcgcaa ggtcgccgcc
     1741 cagagtcaga ccccgaatct gctggggtca gggccggaat ctggtcaagc tttgcgtcag
     1801 caggttttac ggcccagacc ccgaattttg aacttccggg cttatcgagt ccgcttgcct
     1861 ccgcgcctgc gcacgcggta ttcggcggcg ggctcgcgga tcagaagctc gctcggaatg
     1921 ccgagcttct cgtgaagcgc gcgaaccatc gaaagcgtga gaggccgctt gcgtgaaagg
     1981 atctccgacg cccggctttt cccgccgacg atcgcggcga tgtcggcctg gcgcaggccc
     2041 tgctcctcca tccggaagac aatcgcatcg atcgggtcag gcttgcggaa cttgaagcgc
     2101 tctcgctcgt agtcgtcgat gagctttgcg aggagctcga gccgtgcgcc gtcgtcgctt
     2161 tgcggcgccg ggtcgccctt cgcaaggcgc cgcgcctctt ccacgtagcg gcgatgctgc
     2221 tcgccggtct tgatgatccg cagttccatg tcagcgtctc ttgttgcgct cgtcatactc
     2281 ccggtgcgtg ccgatccaat cgatcacgac gacgccggtc cgataggcaa cggtcacttc
     2341 cagccggtac gcgttgccct tcacattgaa gatcacgacg ccctccggca ggaagctcgc
     2401 gctggcgtag cgccggcgaa tgtcctgcgg cgtggtccac gccgcgctct cgacatcggc
     2461 cagccagttc tcgatccagc cgcgggcatc ggtgtgcttg cggcagaacc gctcgagccg
     2521 ctcgcggtcg agtaccttca tccatgcctg gcgattggag ccgctaactt agcacgaagt
     2581 tcccattttg ggaacctctc attggacccc atgtcgagcc caccccggta gcaacagcgg
     2641 ccgcaatcgc gggaacttcc tttttcctga cggttacatc cgggatacac acccgaaatc
     2701 atcccggccg cgcgcgcgcc taagctcccc tccgcacttt caccagccaa cggaggaaac
     2761 acaccatgca aagcgccacc gcggtcgcgt cgaacaccca gccggtcaac cccaacaagg
     2821 ccctctggga gaagggcgac ttcacccgca tcgcggaaac catgcgcgcg agcggcgatg
     2881 cgctcgtcgc aagcctcggc gtcgcgaagg gcatgaaggt gctcgacctc ggctgcggcg
     2941 acggcaccac cgccatcccc gccgcccagc gcggcgccga cgtcctgggc gtcgacatcg
     3001 cggcgaacct cgtcgccgcg ggcaaccgcc gcgcgcaagg gctcggcctc gccaacgtcc
     3061 gcttccagca aggcgacgcc tgcgacctgc agggcgtgcc ggacaagagc ttcgacctcc
     3121 tcgtcagcat cttcggcgcc atgttcgcgc cgcggccgct cgaggtggcg aaggcgatgg
     3181 tgcgcgtcgt gcgcccgggc gggcgcatcg tcatgggcaa ctggatcccg aacgacccga
     3241 ccctcgtcgc gcagatcctc aagatcagct ccgcctacac cccgccgccg cccgccggct
     3301 tcgtgagccc gatgacctgg ggcgtcgagg cgacggtcgc cgagcgcttc gccgccgccg
     3361 gcgtcccggc cgcgaacgtt gccttcgcgc gcgacacctt caccttcgac ttcgccggca
     3421 ccccggccga gctcgtcggc cgcttccgcg acttttacgg ccccaccatg aacgccttcg
     3481 acgccgccca gaagagcggc cgcgtcgccg agctgcagcg cgagctcgag gcgctcttcg
     3541 ccgcgcagaa ctcgagcggg cgcgcggatc ggaccaccat cgctgcgacc tacctgcgcg
     3601 tcagcgtatc cctctgagga agaggagaag cccgccgacc ccctgacaag ggggtcgtga
     3661 gtccccgcgt aggcggggac gagcgggggg ttttcaaagg cctgcctgcg cacgcgggcg
     3721 gcgacccggg gcatgggccc gcgcatgctc gtgctcacgc cgacgcgcga gctggcgctg
     3781 caggtgcaga aagccacgca cggctactgc a
//
LOCUS       DATBSZ010000054        16294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1850914, whole genome shotgun sequence.
ACCESSION   DATBSZ010000054 DATBSZ010000000
VERSION     DATBSZ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16294
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850914"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /gene="mutL"
                     /locus_tag="VIG70_03440"
     CDS             complement(<1..1580)
                     /gene="mutL"
                     /locus_tag="VIG70_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY8249633.1"
                     /translation="MRSIRVLPQLLVSQIAAGEVVERPASVLKELLENSVDAGAQAIT
                     VVLEAGGIKRVQVEDDGGGIARDELPLALARHATSKIASLEDLEGVATMGFRGEALAS
                     IASVSRLSITSRTREAPHGAAISAEGAQVGEVQPAARAQGTTVAVSDLYFNTPARRKF
                     LRTESTEFGHCDEMFRRVALAHANTAFMLKHNGRASQVLRSQTPAERVSALLGETFQH
                     VPIEAQSGALKLSGFAGTPQAAGERADGQYFFVNGRYVRDRLLSHAAREAYSEMLHGE
                     RQPAYVLFLEIDPRAVDVNVHPAKTEVRFRDPRAVHQFVYHALQRALAPGVAEAPVAY
                     ASVSHGAPIQAAFGLAQPAAAYESFMKAASLPVSEKAPPLGYALAQLHGVYILAQNEA
                     GLVLVDMHAAHERIVMEKLKSNLDAGAVQRQSLLVPAVFQAEALDVATAEESREALER
                     LGLELTVSGPNELAVRAAPAALAGGDIVGLARGVLGEVREYGASQVLSSRQNELLATM
                     ACHAAVRAHRALSIAEMNA"
     gene            complement(1577..2059)
                     /locus_tag="VIG70_03445"
     CDS             complement(1577..2059)
                     /locus_tag="VIG70_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY8249634.1"
                     /translation="MSSVVLTSSSVERVREALVRAGLADTIVELPGAARTARAAAEYL
                     GCEVGQIANSLVFRAEATDAALLVMSSGAKRVDVARLAALAGEPVGKADADFVRRHTG
                     FVIGGVAPVGHGLTRTFIERSLAAYRELWAAAGHPHTVFRLTYGELLRITAGAEADLA
                     "
     gene            2091..2744
                     /locus_tag="VIG70_03450"
     CDS             2091..2744
                     /locus_tag="VIG70_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY8249635.1"
                     /translation="MELLAWFWDLVVHLDAHLASFVAAHGAWVYGLLFAIVFCETGLV
                     VTPFLPGDSLLFVAGAVAAAGDMNITAVMATLVAAALCGDNVNYWVGRWIGPRVFHFE
                     RSRWFNPAHLARAHAFYERHGGKTIILARFVPIVRTYVPFVAGVGTMPYLRYLGFCIL
                     GAMVWVVSLCLLGYWFGNQPIVKNNLTAVILLIVAVSVSPGFFAWIRSRRQPRVSPP"
     gene            2762..3403
                     /locus_tag="VIG70_03455"
     CDS             2762..3403
                     /locus_tag="VIG70_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249636.1"
                     /translation="MARPTKKTGPRGILTPRGQGLPPLFMPFGFATSAEDLGEFIVAG
                     ATATAAKEGIRLFGQTSRPVRNTANPFDFTVPTEAGEQYLDLLEVAPLSYVDGSHEKG
                     PGAYYQGEMADRVWSGIQRKASKSGPVIAAIHLLIHPTDWRFHFSDGTLQLIAVRLAK
                     RRHPFSTVVYYAPDDAGWGDLRVVHPIRAKPDDIVTFDEGTARAVLVEPVALQ"
     gene            complement(3411..4580)
                     /locus_tag="VIG70_03460"
     CDS             complement(3411..4580)
                     /locus_tag="VIG70_03460"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY8249637.1"
                     /translation="MAFLLAFSVGVAEAQVVSSRIWPARDYTRLTLESKEAIQYSIFS
                     IKDPERLVLDLETNDLTAALTELNDKVNAEDPYIQGLRVARNRPGVIRLVLDLKTEVK
                     PMVFGLPPIADYGHRLVLDIHPLVAEDPLLALLAESEKRRPAQKPGVARLATIVIDPG
                     HGGEDPGAIGRRGSREKDITLNIAKRLKTLIDGEPNMRALLTRDGDYFLPLGTRVQKA
                     RAVKADLLVSVHADAVNRPNVRGSSVFALSDRRATSEMARILAKQENDSDLIGGVARK
                     QKDDYVNRTIIDLSQTATIDHSLRLGNSVLKRLGTVNDLHKPHVEQASFAVLTAHDVP
                     SILVETAFISNPQEERRLNDEAYQDQLARAILGGIREYIARHPPRPLGPITFSTR"
     gene            complement(4553..5014)
                     /gene="tsaE"
                     /locus_tag="VIG70_03465"
     CDS             complement(4553..5014)
                     /gene="tsaE"
                     /locus_tag="VIG70_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY8249638.1"
                     /translation="MMLELRLPDEASTLRLGEALATGAAAGRVLFISGELGAGKTTLV
                     RGLLRGLGYSGRAKSPTYALVEPYALSSLDLYHFDFFRFKDRSEWLNSGFREHFNPGS
                     LCVVEWPEKAGDLLAPPDLHIRLEIDDGAHRARLEAPSQAGEAWLSSLRFP"
     gene            4920..6029
                     /gene="queG"
                     /locus_tag="VIG70_03470"
     CDS             4920..6029
                     /gene="queG"
                     /locus_tag="VIG70_03470"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY8249639.1"
                     /translation="MKSTRPAAAPVANASPKRRVDASSGNLSSSIKLWGRELGFQAVG
                     IADADLSAAEPRLLEWLGRGWHGEMEYMARHGALRARPAELKPGTLRVISCRMDYLNG
                     EAQGISPDPGKAHIARYARGRDYHKVLRDRLQKLCERIAQEIGEFGYRVFTDSAPVME
                     VELAARAGIGWRGKHTLLLSRDAGSWFFLGEVYTDLPLEVDQEVANSCGTCERCIEVC
                     PTQAIRGPYQLDARRCISYLTIEHKSAIPEELRPLIGNRVYGCDDCQLVCPWNRFAKL
                     TSEGDFRTRNGLDAATLVELFAWTEAEFDSRLRGSPIRRIGHERWLRNLAVGLGNAPT
                     SLHVVAALRSRADHPSALVREHVAWAISRHASLAA"
     gene            6010..6906
                     /locus_tag="VIG70_03475"
     CDS             6010..6906
                     /locus_tag="VIG70_03475"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8249640.1"
                     /translation="MQAWQPDRFLPGFEALELEFPDDYDGRVLATLVRLAGGEAPRGA
                     VLWVHGFVDYFFQSHVAQRFAMEGYAFYALDLRKHGRSLRPHQHPNFCKNVAEYYPDI
                     TRSIDVIGEPVILAGHSTGGLIAALYAHEGPHRSRVQALWLNSPFFDWNMPDWRRTQL
                     HMAAALGRFFPYLNDPKALLPDYTQALLDQGWEFDTALKPVPGFPVYYGWLGAVADAH
                     AKVHRGLDVRGPVLAMHSDEADIVLDWRHIARWSRSLGPEVTVLAFPGAMHDLTLSRP
                     EIREEVFRQLFSWAERAVMSPA"
     gene            complement(6857..7222)
                     /locus_tag="VIG70_03480"
     CDS             complement(6857..7222)
                     /locus_tag="VIG70_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEY8249641.1"
                     /translation="MADAILVVHFLIVIFIVGGLLLVWVGAALGWRWVRNPWFRYLHL
                     GAIAFVAAEALLGVMCPLTVWEDLLRGGVRADTFVGRWVRYFLYYEAPEWVFTAAYVA
                     WTAATLATLRLVPPRKRAA"
     gene            7232..7783
                     /locus_tag="VIG70_03485"
     CDS             7232..7783
                     /locus_tag="VIG70_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY8249642.1"
                     /translation="MAQRFPKLTPDQMTAAQREVAAEITAGPRGEVRGPFIALLHNPE
                     LASKVQKLGEHLRWKGKLPPRLKELAVLVTARRWTCQHEWVMHSKLALEGGLAPQIVD
                     AIKEGKEPTGMSQEEKDVYTFCREVHATGRAGDAAFAAIQQRFGLDGALELVALNGYY
                     TLMAMVLNTAGLPLPGNAEPPLK"
     gene            complement(7785..8594)
                     /locus_tag="VIG70_03490"
     CDS             complement(7785..8594)
                     /locus_tag="VIG70_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8249643.1"
                     /translation="MGRSAVQVSDTWERGSPYEQYVGRWSRRVAPLFLSWLNVPAGRR
                     WLDVGCGTGALCAAIVDRCSPAAVAGVEPSEGFLKTAKENLAGRAALHQGSASAIPLG
                     NASVDVAVSGLVLNFVPDARAALLEMARATGKEGTIAGYVWDYAGKMELMRYFWDAAV
                     ALDPDVAKLDEGVRFPLCRPEALGKLFASASLEDVEVNPIDIPTPFASFDDYWQPFLG
                     GQGPAPAYAMSLDEAARTRLRDRLKERLPTQANGSISLIARAWATRARVAA"
     gene            complement(8587..9420)
                     /locus_tag="VIG70_03495"
     CDS             complement(8587..9420)
                     /locus_tag="VIG70_03495"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY8249644.1"
                     /translation="MNGSTLVSTGELAANLVKWRVFDCRHDLAKPDLGEKQYREGHIP
                     GALFAHLDRDLSARKTGKNGRHPLPDPQDFEKWLEKTGLTPKDQVVCYDGGPGAMAAR
                     LWWMLRWIGHDKVAVLDGGFAKWTKEGRKITVDVPLFTPLNYPIKPRKDFAVDVAAVA
                     KNLDKLFLIDARAPARYRGEQEPIDPVAGRIPGAKNRFNMENLSAEGVFKDQESLRKD
                     FAAVLGKRLPSEVVHYCGSGVAACHNLLAMEIAGLKGGKLYAGSWSEWSADPKRPQER
                     G"
     gene            complement(9389..10486)
                     /locus_tag="VIG70_03500"
     CDS             complement(9389..10486)
                     /locus_tag="VIG70_03500"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY8249645.1"
                     /translation="MADVAPLKQLKPTSCQLPVSWYFDPEIFEAEKRLLFQSGPNYVG
                     HELMVPNAGDWHTLAWMDHAKVLVRNHTGIELLSNVCRHRQAIMLEGRGNAENIVCPV
                     HRWTYDLKGELLGAPRFADQPCVKLASTPLANWNGLLFAGPRDPARDLARITTNADWN
                     FSGYVLDSVRIDEYDINWKTFIETYLEVYHVDFFHPGLGNFTDCNNFTVDYGPDYSVQ
                     IVEAKSGFARPGTPTYKRWHEACLKVLDGREPKQGALWMTYYPGLMLEWYPNVLIVSQ
                     LIPRSPTRTTNVVEFYYPEEIALFEREFIEAQQAAYVETAIEDNEICMRLDRGRRALY
                     EQGLDDAGPYQSPMEDAEVHFHEWLHTRLDR"
     gene            10527..10766
                     /locus_tag="VIG70_03505"
     CDS             10527..10766
                     /locus_tag="VIG70_03505"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY8249646.1"
                     /translation="MSKPSELDFEKALAELEKIVARMESGELSLEQSLATHKRGLELA
                     RFCQQKLEAAQQQVKVLEGEVLKPLAAAAEPDENS"
     gene            10807..11652
                     /locus_tag="VIG70_03510"
     CDS             10807..11652
                     /locus_tag="VIG70_03510"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY8249647.1"
                     /translation="METALARLLPAAHVAPARLHEAMRYATLEGGKRVRPLLVFAAGE
                     VSGAAPERLEIAAAAVELIHAYSLVHDDLPCMDDDTLRRGKPTVHMEFNEATALLVGD
                     ALQSLAFQLISEHRISDDPRVQLEMVKTLAAAAGSRGMAGGQQIDLEATGKTLTLPEL
                     EFMHIHKTGALIRAAVTLGSLCGSPGENERQKLDRYAKAMGLAFQVVDDVLDHDASTA
                     TLGKTAGKDSRQGKPTYVSAMGVARARELAEDLRGEAHAALADLGARARHLAELADFI
                     VLRKF"
     gene            11653..13533
                     /gene="dxs"
                     /locus_tag="VIG70_03515"
     CDS             11653..13533
                     /gene="dxs"
                     /locus_tag="VIG70_03515"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY8249648.1"
                     /translation="MAAVTHELPANGGNPTSYDLLKTIDEPAQLRSLERKQLAQLAAE
                     LRGYVLESVSKTGGHLSSNLGTVELTIALHYVFDTPEDRIVWDVGHQTYPHKILTNRR
                     EAMARLRMLDGISGFPRRSESKYDTFGTAHSSTSISAALGMAVASKIRKETRRCIAVI
                     GDGAMSAGMAYEAMNNAGAMDVDLLVILNDNEMSISPPVGALTNYLARLLSGRIYSTA
                     RRASEHLLKRVGVWELARRTEEHVKGMVTPSTLFEEFGFNYLGPIDGHDLDALIPTLT
                     NIKKLSGPQFLHIVTRKGQGYKLAEEDPIAYHGPSKFNPAEGLQKAPGGKPTYSQVFG
                     DWLCDMATRDPRLVAVTPAMREGSGMVAFSERFPERYFDVGIAEQHAVTFAAGLACEG
                     MKPVVAIYSTFLQRGYDQLIHDVAIQNLPVLFALDRGGLVGGDGATHNGAFDYAYLRT
                     VPNLTVMAPSDAAECRNMLFTGINLGRPAAVRYPRGAALGDVSREMYAITVGKAEIRR
                     AGRRIAILAFGSMLKPALEAGEALDATVVNMRFVKPLDTAVIDELARTHELLVTVEEH
                     QVMGGAGSAVCEALARGTARVLVLGLPDRFIDHGDPGRLLASVGLDAAGIQKAITDVM
                     SE"
     gene            13551..14366
                     /gene="folE2"
                     /locus_tag="VIG70_03520"
     CDS             13551..14366
                     /gene="folE2"
                     /locus_tag="VIG70_03520"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEY8249649.1"
                     /translation="MDLMNIRDLQSIPDVQATPDSRKLAIDKVGIKAIRHPVQIRERS
                     GAVQHTVATFNMYVGLPHQFKGTHMSRFVEILNAHEREITVETFQVMLQEMVQKLEAK
                     EGRIEMSFPYFVDKAAPVSGVRSLMDYEVTFVAQILKGERCFAMKVLVPVTSLCPCSK
                     KISEYGAHNQRSHVTVTAHTREFVWIEEIIDLIEKQASSELYGLLKRPDEKFVTERAY
                     DNPKFVEDMVRDVAAVLNLDSRITRYVVESENFESIHNHSAYALIERDKRSAG"
     gene            14435..15070
                     /locus_tag="VIG70_03525"
     CDS             14435..15070
                     /locus_tag="VIG70_03525"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY8249650.1"
                     /translation="MVAEVDAMYAETRSETGLARMSPAVRGAMGKVSRHRLVASSEAS
                     RAYRNHPLPIGSGQTISQPYIVALSTDLLVPKPTDVVLEVGTGSGYQAAVLAEIVSRV
                     YSIEIIASLGNEARRRLEELGYGNIEVQIGDGYAGWPEKAPFDGIVVTAAAPRVPQPL
                     VDQLKPGGRMVIPVGGAGDIQYLKLLTKRADGGVDEKRVLPVRFVPLVPGK"
     gene            complement(15136..15774)
                     /locus_tag="VIG70_03530"
     CDS             complement(15136..15774)
                     /locus_tag="VIG70_03530"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEY8249651.1"
                     /translation="MTRIIALLLALACGPAAAVDVALIGLGGDRAATLAIGGGEPKMV
                     SVGQTWRGVKVISVEREFGQATVEIDGKQRVLKIGQHYRSAVAASDRQSVTLAADPRG
                     HFYTDGAINGNPVRFLVDTGATMIALPAADAQRLGIDYRKGERGYSSTAGGVVPIYRV
                     RFDTVRLGSIEVTGVDGVVIEQGLDTALLGMTFLNRVEMKRDGQTMVLIRRF"
     gene            15866..>16294
                     /locus_tag="VIG70_03535"
     CDS             15866..>16294
                     /locus_tag="VIG70_03535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEY8249652.1"
                     /translation="MALDQGEPVPRDPRNFSGLAVMGGPMSANDELPWIAPLLELLRN
                     AVRQDVPVIGHCLGGQLMSRAFGGTVRANPVREIGWGEIRVADNGVAREWLGELQSFQ
                     TFQWHGETFSIPPGATRVLEGEHCANQAFAVGRHIGMQCHV"
BASE COUNT         2722 a         5460 c         5438 g         2674 t
ORIGIN      
        1 gcgttcatct ccgcgatgct gagcgcgcga tgggcgcgta ccgcggcatg gcacgccatg
       61 gtcgcgagga gctcgttctg gcgcgacgac agcacctggc tcgcgccgta ctcgcggacc
      121 tcgccgagca ccccgcgcgc cagcccgacg atgtcgccgc cggcgagcgc cgccggcgcc
      181 gcgcgcaccg cgagctcgtt cggtcccgac acggtgagct cgagcccgag gcgctcgagc
      241 gcctcgcggc tctcctcggc ggtggcgacg tcgagcgcct cggcctggaa caccgccggc
      301 accagcaggc tctggcgctg caccgcgccg gcgtcgaggt tggacttgag cttctccatc
      361 acgatgcgct cgtgcgccgc gtgcatgtcg accagcacga ggccggcctc gttctgcgcg
      421 aggatgtaca cgccgtgcag ttgcgcgagg gcataaccca gcgggggcgc cttttcgctg
      481 actggcaatg atgctgcctt catgaacgac tcgtaagcag ccgccggctg cgcaaggccg
      541 aaggccgcct ggatgggagc cccgtgagaa acagatgcat aggccaccgg cgcctccgcc
      601 accccgggcg ccagcgcccg ctgcagcgcg tggtagacga actggtgcac cgcgcgcggg
      661 tcgcggaagc gcacctccgt cttggccggg tgcacgttga cgtccactgc gcgcggatcg
      721 atctccagga acagcacgta cgcgggctgg cgctcgccgt ggagcatctc cgagtaggct
      781 tcgcgcgccg cgtgcgacag cagccggtcg cgcacatagc gtccattgac gaagaagtac
      841 tgtccgtcgg cgcgctcgcc cgcggcctgg ggcgtgccgg cgaagccgga gagcttcagc
      901 gcaccggact gggcttcgat ggggacgtgc tgaaacgtct ctcccagcaa agccgagacg
      961 cgctcggctg gcgtctggct gcgcagcacc tggctcgcgc ggccgttgtg cttcagcatg
     1021 aacgcggtat tcgcgtgcgc gagcgcgacg cgccggaaca tctcgtcgca atggccgaac
     1081 tcggtcgact cggtgcgcag gaacttgcgc cgcgccggcg tattgaaata gaggtccgac
     1141 accgcgacgg tcgtgccttg cgcgcgcgcc gcgggctgca cctcgccgac ctgcgccccc
     1201 tcggccgaga tcgccgcgcc gtgcggcgcc tcgcgcgtgc ggctggtgat ggagaggcgc
     1261 gacaccgagg cgatcgaggc gagcgcctcg ccgcggaatc ccatggtcgc cacgccctcg
     1321 aggtcctcca ggctcgcgat cttgctggtg gcgtggcgcg cgagcgcgag cggcagctcg
     1381 tcgcgcgcga tgccgccgcc gtcgtcctcg acctgcacgc gcttgattcc gcccgcttcg
     1441 agcaccaccg tgatcgcctg cgcgccggcg tcgacgctgt tctcgagcag ctccttcagt
     1501 acggacgcgg ggcgctcgac cacctcgccc gccgcgatct ggctgacgag gagctggggc
     1561 aggacgcgga tgcttctcat gcgagatcgg cctcggcgcc ggcggtgatg cgcagcagct
     1621 cgccgtaagt caggcgaaag acggtatggg gatggccggc ggcggcccag agctcccggt
     1681 acgccgccag gcttctctcg atgaacgtcc gggtgagccc atggccgacc ggcgcgacgc
     1741 cgccgatcac gaagccggta tggcggcgca cgaagtccgc atccgccttg ccgaccggct
     1801 cgccggcgag tgccgccagc cgcgcgacat cgacgcgctt cgcgcctgag gacatgacga
     1861 gcagcgcggc gtcggtggct tcggcgcgaa acacgagcga gttagcaatt tgtccgacct
     1921 cgcagcccag gtactcggcc gccgcccgtg cggtgcgcgc ggcgcccggc agctcgacga
     1981 tggtgtccgc gagccccgcg cgcaccagcg cttcacgcac gcgttcgacg ctcgaggaag
     2041 tgagaacgac ggaggacatg caagggagcg gccgattata caatcgcgcg atggagctgc
     2101 tcgcctggtt ctgggatctg gtggtgcatc tcgacgcgca tctggcgagc ttcgtcgcgg
     2161 cgcacggcgc ctgggtatac ggcttgcttt tcgcgatcgt gttctgcgaa accggccttg
     2221 tcgtcacccc gttcctgccg ggcgattctc tgctgttcgt cgccggggcg gtcgccgccg
     2281 cgggcgacat gaatatcacc gccgtcatgg cgacgctcgt cgccgcagcg ctctgcggcg
     2341 acaacgtcaa ctactgggtc ggccgctgga tcggcccccg cgtgttccac ttcgagcgct
     2401 cgcgctggtt caacccggcg cacctcgcgc gcgcgcacgc tttctacgag cgacacggcg
     2461 gcaagaccat catccttgcg cgcttcgtcc cgatcgtgcg cacctacgtg ccgttcgtgg
     2521 cgggcgtggg cacaatgccc tacctgcgct atctcggctt ctgcatactc ggcgccatgg
     2581 tctgggtggt ttcgctctgc ctgctgggct actggtttgg taaccagccg atcgtcaaga
     2641 acaacctgac cgccgtcatc ctcctgatcg tcgccgtctc ggtctcgccc ggtttcttcg
     2701 cctggatacg ctcgcgccgc cagccgcgcg tttctccccc ctagagcggc agggtcgatc
     2761 gatggcacga ccgacgaaaa aaaccggacc ccgcggcatc ctgacgccgc gcggccaggg
     2821 cctgccgccg ctcttcatgc cgttcggatt tgcgacctcg gcggaggacc tcggggaatt
     2881 cattgtcgcg ggggccaccg caaccgcggc gaaggaaggg atccggttat tcggccagac
     2941 ctcgagaccg gtgagaaaca cggcgaaccc cttcgacttc acggttccta ccgaagccgg
     3001 cgagcagtac ctggatctgc ttgaagtcgc gccgctctcc tatgtcgacg gttcgcatga
     3061 gaaagggccc ggggcgtact accagggcga gatggccgat cgggtctggt ccgggatcca
     3121 gcgcaaggcc tccaaatccg gaccggtcat cgccgcgatc cacctgctga ttcacccgac
     3181 cgattggcga ttccacttca gcgacggcac gctgcagctg atcgccgtgc gcctggccaa
     3241 gcggcgacat ccgttctcga ccgtcgtcta ctacgctccc gacgacgccg ggtggggtga
     3301 tctgagagtc gtgcatccga tccgcgcaaa gcccgacgac atcgtgacct ttgacgaggg
     3361 cacggcacgc gccgtgctgg tcgagcccgt cgccctgcag tgaagacact ctatcgagta
     3421 gaaaacgtga tcggccccag cgggcgcggc ggatgcctgg cgatgtactc ccggatgccg
     3481 ccgaggatcg cccgggcgag ctggtcctgg taggcctcgt cgttcaggcg gcgctcctcc
     3541 tgcgggttgg agatgaaggc ggtttcgacc aggatcgacg gcacgtcgtg cgcggtgagc
     3601 accgcgaagc tcgcctgctc gacgtggggc ttgtgcaggt cgttgacggt cccgaggcgc
     3661 ttcagcaccg aatttccgag ccgcaggctg tggtcgatcg tggcggtctg cgacagatcg
     3721 atgatcgtcc ggttgacgta gtcgtccttc tgcttgcggg cgacgccgcc gatcaggtcg
     3781 gagtcgtttt cctgcttggc gaggatgcgc gccatctcgg aagtggcgcg gcgatccgac
     3841 agcgcgaaga cggaagagcc gcgcacgttc ggccgattga ccgcgtcggc gtggaccgag
     3901 accagcaggt cggccttgac cgcgcgcgcc ttttggacgc gcgtgccgag cgggagaaag
     3961 tagtcgccat cccgggtgag cagcgcgcgc atgttgggct cgccgtcgat cagcgtcttg
     4021 aggcgcttcg cgatgttgag ggtgatgtcc ttctcgcgcg agccgcgccg gccgatggcg
     4081 cccgggtcct cgccgccgtg ccccggatcg atgacgatcg tggcgagccg ggcgacgccg
     4141 ggcttttgcg caggtcgcct tttctcgctc tccgcgagca acgccaggag cggatcctcc
     4201 gccaccagcg gatggatatc gagcaccagc cggtggccgt agtcggcgat cggcgggagc
     4261 ccgaacacca tgggcttcac ctcggtcttg agatcgagga cgagacggat cacgccggga
     4321 cggttgcgcg ccacgcgcaa gccctggatg tagggatctt cggcgttgac cttgtcgttc
     4381 agctcggtca gcgccgcggt caagtcgttc gtttccaggt cgagcaccag gcgctcggga
     4441 tccttgatgc tgaagatgct gtactggatc gcttccttcg attcgagcgt gaggcgggtg
     4501 taatcgcgcg cgggccagat gcgcgaggag acgacctggg cttcggcgac gcctacggaa
     4561 aacgcaagga ggaaagccag gcttcgccag cttgcgaggg cgcttccagc cgcgcgcggt
     4621 gcgctccgtc gtcgatctcc agcctgatgt gcaagtccgg cggcgccagc aggtccccgg
     4681 ctttctccgg ccattcgacc acgcacagcg agcccgggtt gaagtgctcc cggaagcccg
     4741 aattcagcca ttccgaccga tccttgaatc gaaagaaatc aaagtgatat aagtctaagc
     4801 tcgaaagcgc gtaaggctca accaaggcat aggtgggact cttggcccgg ccggaatacc
     4861 ccagcccccg cagcagcccc ctgaccagcg tagtcttgcc cgcgccgagc tcacccgaga
     4921 tgaaaagcac ccgtccagcg gccgctcccg ttgccaacgc ttcgcccaag cgcagggttg
     4981 acgcctcgtc gggcaatcta agctcgagca tcaagctttg gggccgggag ctcgggttcc
     5041 aggcggtcgg gattgccgac gccgaccttt cggcggccga gccgcggctc cttgagtggc
     5101 tgggacgggg ctggcatggc gagatggagt atatggcgcg acacggagcg ctccgcgcgc
     5161 gccccgccga gctcaagccc gggactctgc gcgtaatttc ctgccgcatg gactacctga
     5221 atggcgaagc ccagggaatt tccccagacc cgggaaaagc acacatagcc cgctacgcga
     5281 gaggccgcga ctatcacaag gtgctgcgcg accggctgca gaagctctgc gagcgcatcg
     5341 cgcaggagat cggcgagttc ggctatcgcg tgttcaccga ctcggcgcca gtgatggaag
     5401 tcgagctcgc cgcgcgcgcc ggcatagggt ggcgcggcaa gcacacgcta cttctcagcc
     5461 gcgatgccgg gtcctggttc ttcctcggcg aggtgtacac ggatttgcca ctcgaagtcg
     5521 accaggaggt cgccaattcc tgtggaacct gcgagcgctg catcgaggtg tgccccaccc
     5581 aggcgatccg cggcccctac cagctcgacg ctcgccgctg catctcctac ctcaccatcg
     5641 agcacaagag cgccatcccc gaggagctgc ggccgctcat cggcaaccgg gtctacggct
     5701 gcgacgactg ccagctggtg tgcccgtgga accggtttgc caagctgacc tccgaagggg
     5761 atttccggac gagaaacggc ctcgacgccg ccacgctggt ggagctcttc gcgtggaccg
     5821 aggccgagtt cgacagccgc ctgcgcggct cgccgatccg gcgcatcggc cacgagcgct
     5881 ggctgcgcaa cctcgccgtc gggctgggca atgcgccgac ttcgttacac gtggtggccg
     5941 cattacgctc ccgggcggac catccctccg cgctagtcag ggagcatgtc gcttgggcaa
     6001 tctctcggca tgcaagcctg gcagcctgac cgcttcctgc ccgggttcga ggcgctcgag
     6061 ctcgagttcc ccgacgacta cgacgggcgg gtgctcgcga cgctggtgcg cctggccgga
     6121 ggcgaggcgc cgcgcggcgc ggtgctgtgg gtccacggct tcgtcgacta ctttttccag
     6181 agccacgtcg cgcagcgctt cgcgatggag ggctacgcct tctacgcgct cgacttgcgc
     6241 aagcacgggc gctcgctgcg gccgcaccag catcccaact tctgcaagaa cgtcgccgag
     6301 tactacccgg acatcacgcg ctcgatcgac gtcatcggcg agccggtgat cctcgccggg
     6361 cattccaccg gcggcctgat cgccgcgctc tacgcccacg aagggccgca ccgcagccgc
     6421 gtgcaggcgc tctggctcaa cagcccgttc ttcgactgga acatgcccga ctggcggcgc
     6481 acgcagctcc acatggcggc ggcgctcggc cgtttctttc cctacctcaa cgaccccaag
     6541 gcgctgctcc cggactacac ccaggcgttg ctcgaccagg gctgggaatt cgacaccgcg
     6601 ctcaagcccg tgcccggctt cccggtctac tacggctggc tcggcgcggt cgccgacgcc
     6661 cacgccaagg tgcaccgcgg cctggacgtg cgcggcccgg tgctggcgat gcattcggac
     6721 gaggccgaca tcgtgctcga ctggcgccac atcgcgcgct ggtcgcgcag cctcggcccc
     6781 gaggtcaccg tgctcgcgtt tccgggcgcc atgcacgacc tgaccctctc gcgccccgag
     6841 atccgcgagg aggtcttcag gcagctcttt tcctgggcgg aacgagccgt aatgtcgcca
     6901 gcgtagcggc ggtccaggcg acgtaggcgg ccgtgaacac ccattccggc gcctcgtagt
     6961 acaggaagta gcgcacccag cggccgacga acgtgtcggc gcgcaccccg ccgcgcagca
     7021 ggtcttccca cacggtcagc gggcacatca ccccgagcag cgcttccgcg gccacgaagg
     7081 cgatcgcgcc gaggtgcagg tagcggaacc acggattgcg cacccagcgc cagccgagcg
     7141 cggcgccgac ccacaccagc agcaggccgc cgacgatgaa gataacgatc aggaagtgga
     7201 cgacgaggat ggcgtccgcc atgcttctag catggcacaa cgatttccga agctcacccc
     7261 cgaccagatg accgccgcgc agcgcgaagt cgccgccgag atcaccgccg gcccgcgcgg
     7321 cgaggtgcgc ggccccttca tcgcgctcct ccacaacccc gagctcgcga gcaaagtgca
     7381 gaaactgggc gagcatctgc gctggaaagg caagctgccg ccgcgcctca aggaactggc
     7441 ggtgctggtg accgcccggc gctggacctg ccagcacgag tgggtgatgc attccaagct
     7501 cgcgctggag ggtggcctcg cgccccagat cgtggacgcg atcaaggagg gaaaagagcc
     7561 aaccgggatg tcgcaggagg aaaaggacgt ctacacgttc tgccgcgagg tgcacgcgac
     7621 gggccgggca ggggacgccg ccttcgccgc gatccagcaa cgcttcgggc tcgacggcgc
     7681 tctcgagctc gttgcgctca acggctacta cacgctgatg gcgatggtgc tcaacaccgc
     7741 cggcttgccg ctaccgggaa atgctgagcc gccgttgaag taacttacgc tgcgactcgg
     7801 gcgcgagttg cccaggcgcg agcaatcaaa gagatcgacc cgttcgcttg cgtcggcagg
     7861 cgctctttta aacggtcccg taggcgcgtc ctcgctgcct catcgagcga catggcgtag
     7921 gcgggagccg gcccttggcc gcccaggaag ggttgccagt agtcatcgaa gctcgcaaac
     7981 ggcgttggta tgtcgatcgg atttacttcg acatcctcca ggctggcgct ggcgaagagt
     8041 ttcccgagcg cctccgggcg gcacagcggg aagcggacgc cttcgtccag cttcgcgacg
     8101 tccgggtcca gtgccaccgc cgcatcccag aagtaccgca tcaactccat ctttccggcg
     8161 tagtcccaga cgtatcctgc gatcgttcct tccttgcccg tcgcccgcgc catctcaagg
     8221 agcgcggcgc gcgcatccgg aacgaagttc aggaccagtc cggaaaccgc cacatcgacc
     8281 gaggcgttgc cgagcggaat cgcgctcgcg ctgccttggt gaagcgcggc gcgaccggcg
     8341 aggttctcct ttgcggtctt gagaaatccc tccgacggct cgacgccggc gaccgcggcg
     8401 ggggagcagc ggtccacgat tgccgcgcat aacgctcccg tgccgcatcc cacgtcgagc
     8461 catctgcggc cggccggaac gttcaaccac gagagaaaca ggggagcgac ccgccggctc
     8521 caacggccga catactgttc gtatggactt ccgcgctccc aggtgtcgga gacctgcacg
     8581 gcggatctac ccacgttcct gcgggcgctt cgggtcggcg ctccactcgc tccacgagcc
     8641 ggcgtacagc ttgccgcctt tcaggccggc gatctccatc gcgagcaggt tgtggcacgc
     8701 ggcgacgcct gagccgcagt agtggactac ctcagagggc aaacgcttgc ccaggacagc
     8761 ggcaaagtct ttccgcaggg attcctggtc cttgaaaact ccctcggccg agaggttctc
     8821 catgttgaag cggttcttcg cgccggggat gcggccggcg acaggatcga tcggctcctg
     8881 ctcaccgcgg tagcgcgccg gggcgcgggc gtcgatgagg aacagcttgt cgaggttctt
     8941 tgccaccgcg gcaacgtcga cggcgaagtc cttgcgcggc ttgatcgggt agttgagagg
     9001 cgtgaagagc ggcacgtcga ccgtgatctt gcgtccttcc ttcgtccact tcgcaaagcc
     9061 gccatcgagc acggcgacct tgtcgtgccc gatccagcgc agcatccacc acaggcgcgc
     9121 ggccatggcg ccgggaccgc cgtcgtagca gacgacctgg tccttggggg tcaggccggt
     9181 cttctcgagc catttttcga agtcctgcgg gtcgggcagg ggatggcggc cgttctttcc
     9241 ggtcttgcgc gcggagaggt cgcggtcgag gtgcgcgaat agcgcccccg gaatatggcc
     9301 ttcgcggtac tgcttctcgc ccaggtcagg cttggcgagg tcgtggcggc agtcgaagac
     9361 ccgccacttg accaggttgg cggctagctc accggtcgag acgagtgtgg agccattcat
     9421 ggaagtgcac ctctgcgtct tccatcgggg attgatacgg gccggcgtcg tcgagtcctt
     9481 gctcgtacag tgcgcgccgg ccgcggtcca ggcgcatgca gatctcgttg tcctcgatcg
     9541 cggtctcgac gtaggcggct tgctgcgcct cgatgaactc gcgctcgaag agggcgattt
     9601 cctcgggata gtagaactcg accacgttgg tagtgcgcgt gggcgagcgc gggatgagtt
     9661 gcgacacgat aagcacgttc gggtaccact cgagcatcag cccggggtaa taggtcatcc
     9721 acagcgcgcc ctgctttggt tcgcgtccat cgagaacctt gaggcaggct tcgtgccagc
     9781 gcttgtacgt cggcgtcccc ggccgggcga acccggactt ggcttcgacg atctgcaccg
     9841 agtagtccgg gccgtagtcg accgtgaagt tgttgcaatc ggtgaaattg ccgagcccgg
     9901 gatggaagaa atcgacgtgg tagacctcga ggtaggtctc gatgaaggtc ttccagttga
     9961 tgtcgtactc gtcgatgcgc accgagtcga gcacgtagcc gctgaagttc cagtcggcgt
    10021 tggtagtgat gcgcgcgagg tcgcgggcgg gatcgcgcgg accggcgaac agcagcccgt
    10081 tccagttcgc gagcggggtc gaagcgagct tgacgcaggg ctggtcggcg aagcgcggcg
    10141 cgccgagcag ctcgcccttc aggtcgtagg tccagcgatg caccgggcaa acgatgttct
    10201 cggcgttgcc gcggccttcc agcatgatcg cctggcgatg gcggcagacg ttcgacagga
    10261 gctcgatccc ggtgtgattc ctgaccagaa ccttggcgtg gtccatccag gctagcgtgt
    10321 gccagtcgcc tgcgttcggg accatcaact cgtggccgac gtagttcggc ccggactgga
    10381 acagaaggcg cttttccgcc tcgaagatct ccgggtcgaa gtaccaggag accggcaact
    10441 gacaactcgt tggttttagc tgcttgagtg gtgcaacatc cgccatgcgt tgtaaagccc
    10501 tcaagaaaac agtattttag caaacgatga gcaagccttc agagctcgac ttcgagaagg
    10561 cgcttgccga gctcgagaaa atcgtcgcgc gcatggaaag cggcgagctt tcgctcgagc
    10621 aatcgctcgc gacccacaag cgcggcctcg agctcgcgcg gttctgccag cagaagctgg
    10681 aggcggctca gcagcaggta aaggtgctcg agggagaagt tttgaagccc ttggctgcgg
    10741 cggccgagcc cgatgaaaat tcttgacagc gaattccagg cctggatggg cggggtccag
    10801 gcgcggatgg aaaccgcgct ggcgcggctg ctgccggcgg ctcatgtcgc gcccgcgcgg
    10861 ctgcacgaag cgatgcgcta cgcgacgctg gagggcggca agcgcgtgcg cccgctgctc
    10921 gtgttcgcgg cgggcgaggt ctccggcgcc gctcccgagc ggctcgagat cgcggccgcc
    10981 gcggtggagc tgatccacgc ctactcgctc gtgcacgacg atttgccctg catggacgac
    11041 gataccctgc gccgcggcaa gccgaccgtg cacatggaat tcaacgaggc gaccgcgctc
    11101 ctggtgggcg acgcgctcca gtcgctcgct ttccagctta tttcggagca caggatttcc
    11161 gatgacccgc gagtgcagct cgagatggtg aagaccctgg ccgccgccgc cggctcgcgc
    11221 ggcatggcgg gcggccagca gatcgacctc gaagccaccg ggaaaacgct cacgttgccg
    11281 gagctcgagt tcatgcacat tcacaagacc ggagcgctga tccgggcggc cgtaacgctg
    11341 ggaagcttgt gtggttcacc aggagaaaat gaaaggcaaa agctcgaccg ttatgccaag
    11401 gccatggggc tcgccttcca ggtggtcgac gacgtgctcg accacgacgc gagcaccgcg
    11461 acgctgggca agacggcggg caaggattcg aggcagggca agccgacgta cgtctcggcc
    11521 atgggtgtgg cgcgcgcccg cgagctcgcc gaggacctcc gcggcgaggc ccatgcggcg
    11581 ctcgcagacc tgggcgcgcg cgcgcgccac cttgccgagc tcgccgattt catcgttctg
    11641 agaaaattct agatggccgc cgttacccac gagctgccgg ccaacggcgg caacccgacg
    11701 agctacgacc tgctcaagac gatcgacgag ccggcgcagc tgcgctcgct cgagcgcaag
    11761 cagctcgccc agctcgccgc cgagctgcgc ggctatgtgc tcgagtcggt ctcgaagacg
    11821 ggcgggcacc tgtcttcgaa cctcggcacc gtggagctga cgatcgcgct gcactacgtt
    11881 ttcgacacgc ccgaggaccg catcgtgtgg gacgtcggcc accagaccta cccgcacaag
    11941 attctcacca accgccgcga agccatggcg cgccttcgca tgctcgacgg catttcgggc
    12001 tttccgcggc gctcggaatc gaagtacgac accttcggca cggcgcattc gagcacttcc
    12061 atttcggcgg cgctcggaat ggcagtggct tcaaaaatac gaaaagaaac tcgacgctgc
    12121 atcgccgtca tcggcgacgg cgcgatgtcg gccggcatgg cctacgaggc gatgaacaac
    12181 gccggcgcca tggacgtcga cctgctggtc atcctgaacg acaacgagat gtcgatctcg
    12241 ccgccggtcg gcgcgctcac caactacctc gcgcggctcc tgtccggccg catctattcg
    12301 accgcgcggc gggcgagcga gcacctgctg aagcgcgtcg gcgtgtggga gctcgcgcgg
    12361 cgcaccgagg agcacgtgaa gggcatggtc acgccctcca cgttgttcga ggagttcggc
    12421 ttcaactacc tcgggccgat cgacggccac gacctcgacg cgctgatccc gaccctgacc
    12481 aacatcaaga agctctccgg gccgcagttc ctgcacatcg tcacgcgcaa ggggcagggc
    12541 tacaagctcg ccgaggaaga cccgatcgcc taccacggtc cgagcaagtt caatcctgca
    12601 gaagggctgc agaaggcgcc gggcgggaaa cctacttatt cgcaagtctt cggcgactgg
    12661 ctgtgcgaca tggcgacgcg cgatccgcgc ctggtcgcgg tcacgccggc gatgcgcgaa
    12721 ggctcgggca tggtggcgtt ctccgagcgc ttcccggagc gctatttcga cgtcggcatc
    12781 gccgagcagc acgccgtcac gtttgccgcc gggctcgcct gcgaaggcat gaagccggtg
    12841 gtcgcgatct attcgacttt cctgcagcgc ggctacgacc agctgatcca cgacgtcgcc
    12901 atccagaacc tgccggtgct gttcgcgctc gaccgcggcg gcctggtcgg cggggacggc
    12961 gccacccaca acggcgcctt cgactatgcc tacctgcgta ccgtgccgaa cctcacggtg
    13021 atggcgccat cggatgccgc ggagtgccgc aacatgctgt tcaccggaat caacctgggc
    13081 cggccggcgg cggtgcgcta tccgcgcggc gccgcgctcg gcgatgtgtc gcgcgagatg
    13141 tacgcgatta ccgtgggcaa ggcggaaatc cggcgcgcag gccggcgcat cgccatcctc
    13201 gcgttcggct cgatgctcaa gcccgcgctc gaggcgggcg aagccctcga cgctaccgtg
    13261 gtcaacatga ggttcgtgaa gccgctggac accgcggtaa tcgatgaact ggctaggacg
    13321 cacgagctgc tcgtgaccgt ggaggagcac caggtgatgg gcggggcggg ttccgcggtc
    13381 tgcgaggcgc ttgcaagggg aacggccagg gtgctggtcc tcggcctgcc ggaccgcttc
    13441 atcgaccatg gcgaccccgg aaggctgctt gcttcggtag gattggatgc cgcaggaatc
    13501 caaaaagcga ttacggacgt aatgtccgag taatttactc ggatgttaaa atggacctca
    13561 tgaacatacg cgacctgcag agcatccccg acgtccaggc caccccggac tcgcgcaagc
    13621 tcgccatcga caaggtgggg atcaaggcca tccggcaccc ggtccagatc agggagcgct
    13681 ccggcgcggt gcagcacacg gtagccacct tcaacatgta cgtcggcctg ccgcaccagt
    13741 tcaagggcac gcacatgtcg cgcttcgtcg agatcctgaa cgcgcacgag cgcgaaatca
    13801 cggtcgagac cttccaggtg atgctccagg aaatggtgca gaagctcgag gccaaggaag
    13861 gccgcatcga gatgagcttc ccgtacttcg tcgacaaggc tgcgccggtg tccggtgtgc
    13921 gcagcctgat ggactacgag gtgacgttcg tcgcccagat cctgaagggc gagcgctgct
    13981 tcgccatgaa ggtgctggtg cccgtaacga gcctgtgccc ctgctcgaag aagatctccg
    14041 agtacggcgc gcacaaccag cgctcgcacg tcaccgtcac cgcgcacacc agggaattcg
    14101 tctggatcga agagattatc gatctcatcg aaaagcaggc ctcgagcgag ctctacgggc
    14161 tgctcaagcg ccccgacgag aagttcgtca ccgagcgcgc ctacgacaac ccgaagttcg
    14221 tcgaagacat ggtgcgcgac gtcgccgcgg tgctcaacct cgacagccgc atcacccgct
    14281 acgtggtgga gtcggagaac ttcgagtcga tccacaacca cagcgcctac gccctgatcg
    14341 agagggacaa acgcagcgcg ggctagcgct tctcgccctg gttatgctcg gattaacggc
    14401 agagggccag gacttcacaa gcgagcgagc ccgcatggtg gccgaggtgg acgcgatgta
    14461 cgcggaaacc cgctcggaga ccggcctcgc gcggatgtcg ccggcggtgc gcggcgcgat
    14521 gggcaaggtc tcgcgccacc ggctcgtcgc atcgagcgaa gcgtcgcgag cgtaccgcaa
    14581 ccacccgttg ccgatcggca gcggccagac catctcgcag ccctacatcg tcgcgctttc
    14641 caccgatctg ctcgtgccga agccgaccga cgtggtcctc gaagtcggaa cgggctcggg
    14701 ctaccaggcg gcggtgctcg cggagatcgt gtcgcgcgtg tattcaatcg agatcattgc
    14761 ctcgctcggg aacgaggcgc gcaggcgcct ggaagagctc ggctacggca atatcgaagt
    14821 acagatcggc gacggctacg ccggctggcc ggagaaggcg ccgttcgacg gcatcgtggt
    14881 gaccgccgcg gcgccgcgcg tgccgcaacc gctcgtcgac cagctcaagc ccggcggccg
    14941 catggtgatc ccggtcggcg gcgcgggcga catccagtat ctcaagctgc tgaccaagcg
    15001 cgccgacggc ggcgtcgacg agaagcgggt gcttcccgtg cggttcgtac ccctggttcc
    15061 tgggaagtga attcggggac ggagccctaa ctccgatagc tgcgcgtcgc cggagttagg
    15121 gctccgtccc cgaattcaga agcggcggat cagcaccatc gtctgcccgt cgcgcttcat
    15181 ctccacgcga ttcaggaagg tcatgccgag gagggccgtg tcgaggccct gctcgatcac
    15241 cacgccgtcg acgccggtga cctcgatcga acccagccgc acggtgtcga acctcacgcg
    15301 gtagatcggc actacgccgc cggcggtgct gctgtagccg cgctcgcctt tgcggtagtc
    15361 gattcccagc cgctgcgcgt cggctgccgg cagggcgatc atcgtcgctc cggtgtcgac
    15421 gaggaagcgg accgggttgc cattgatcgc gccgtcggta tagaaatgcc cgcgcgggtc
    15481 ggcggcgagc gtgacgctct ggcggtcgct ggccgcgacc gcggagcggt agtgctggcc
    15541 gatcttgagc acgcgctgct tgccgtcgat ctcgaccgtg gcctggccga actcgcgctc
    15601 gaccgagatc acctttacgc ctctccacgt ctgcccgacg ctgaccatct tgggctcgcc
    15661 gccgccgatc gcgagcgtcg cggcccggtc gccgcccagc ccgatcaggg cgacgtcgac
    15721 cgcagcggca gggccgcagg cgagagcgag gagaagcgca ataatacggg tcatgaaacc
    15781 agtcggtatt tttagagcag cccgcacgga agggccaggg tattttgcca cctacctcga
    15841 gcggcgctca attccgtgga aagtcgtggc cctcgaccag ggcgagccgg tgccgcgcga
    15901 cccgaggaat ttttccggtc tggcggtgat gggcgggccg atgagcgcga acgacgagct
    15961 gccctggatc gcgccgctcc tcgagctcct gcgcaacgct gtacgccagg acgtgccggt
    16021 gatcgggcac tgcctcggcg ggcagctcat gtcgcgcgcc ttcggcggga ccgtgcgcgc
    16081 gaacccggtg cgcgagatcg gctggggcga aatccgggtg gccgacaacg gcgtcgcgcg
    16141 cgagtggctc ggcgagctcc agtcgttcca gaccttccag tggcacggcg agaccttcag
    16201 catcccaccc ggcgccacgc gcgtgctcga gggcgagcac tgcgcgaacc aggccttcgc
    16261 cgtcggcagg cacatcggca tgcaatgcca cgtc
//
LOCUS       DATBSZ010000055         7638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1510113, whole genome shotgun sequence.
ACCESSION   DATBSZ010000055 DATBSZ010000000
VERSION     DATBSZ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7638
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510113"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VIG70_03540"
     CDS             complement(<1..254)
                     /locus_tag="VIG70_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HEY8249653.1"
                     /translation="MKAERPGRGGVHEALAHDSAGLHVAGEARYIDDLPEPPGTLYAA
                     IGMSECAHGRIRSLDLEPVRRSSGVVAVVAAGDIPGANNIG"
     gene            complement(251..1627)
                     /gene="xdhA"
                     /locus_tag="VIG70_03545"
     CDS             complement(251..1627)
                     /gene="xdhA"
                     /locus_tag="VIG70_03545"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HEY8249654.1"
                     /translation="MRTSLDFCLNGEALSLSGEDPTRTLLTWLRERRGLVGTKEGCAE
                     GDCGACTVVLAQLDADGKTQYLPVNSCILCLGAVDGKEVVTVEGLKGEHPVQRAMVDC
                     HGSQCGFCTPGFVMAMYAHYKRADGASPADAIAGNLCRCTGYRPILEACEQSRALADP
                     KRDLAENQAQLSRLARLPGGSFRVMNFFAPASVAELAAFLLEHPQATILAGGTDVGLW
                     ITKQHRDLDPVAYVGRVAELRVLEEEEDCVEIGAAVTYEDAYAALAGIHPDIGELVRR
                     LGAAQVRATGTLGGNIANGSPIGDTMPVLLALGASVALQRGQQQRTVALDDFYTGYRK
                     NVLQPGEFIRAIRIPKLKRAARFAVYKLSKRFDQDISAVCAAFHVSGNEVRFGFGGMA
                     ATPARAPRAEKMWPQGVEAACSALAEDFKPISDMRASSWYRLAAAQGLLRKFHATVGP
                     RALLEMQP"
     gene            1701..2387
                     /locus_tag="VIG70_03550"
     CDS             1701..2387
                     /locus_tag="VIG70_03550"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8249655.1"
                     /translation="MPRAATRNGRAEERVFNAILDAVLDHRLAPGTKLKERELAEIFG
                     ASRAAVRAALTRLGHSLLVELRPNRGAVIANPSVAETREMFEARRVIESAIVQRLARA
                     LSAKQIAELRAFVAEESRAYRKGDHKAGQRLSIRFHKVLSELAGNRTLDRFMEHLICR
                     TPLLALARTGRIAYCGADEHREVVEALARRDAAAAARCMTQHLNNVEGELVAEPEPKP
                     ESSLAAALTA"
     gene            2384..3223
                     /locus_tag="VIG70_03555"
     CDS             2384..3223
                     /locus_tag="VIG70_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY8249656.1"
                     /translation="MRRFEYFEPATLGEATQILSAGGHVLAGGTDLLVEMKEQLRRVD
                     RVVNIKKIPGMAGLTYDEREGLRIGALVTAREVELSSIVKEKYSGLVQAVRELGSIQV
                     RNRATIVGNVCRASPSADTLPPLIADAALAEIHGRTGTRKVLLEEFFTGPGKTVLGAD
                     ELMTAIVLPPPAPRTGKVYIKHGRRKAMELATVGVAVSLTLNAEVRVVLGAVAPTPIR
                     ARKAEEVLRGRKLNEELIEKAAQAAADESRPISNVRASAGYRRDMVRVLTRRALEQAM
                     RCC"
     gene            3214..3663
                     /locus_tag="VIG70_03560"
     CDS             3214..3663
                     /locus_tag="VIG70_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8249657.1"
                     /translation="MLLKLKINGEARELAVEPYRSLLDVLRNEAGLTGTKKGCDVGDC
                     GACTVIVDGKPVNSCLMLGVEAQGCEVLTIEGLRHPLQESFIRHGGAQCGFCTPGMIM
                     MAKALLDENPRPSEEEVRFAISGNICRCTGYTKIVEAILHVSHRHER"
     gene            3638..5923
                     /locus_tag="VIG70_03565"
     CDS             3638..5923
                     /locus_tag="VIG70_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8249658.1"
                     /translation="MSVIGTSVRRPDVPGKVTGAARYAGDLVLSGMLHAKIKRCAIAH
                     ANIARIDTARALAYPGVKAVLTHLNVPRVLHYGSPHPRSASLARDQYILDSKVRYWGE
                     GVAAVAAASEEIAEEALDLIEVEYQELPAAISVEQALAADAPSIHGTKSNLVTDPVLV
                     KRGDIDRGFAEADLVVEGTYEMGRPTPAYMEPNVCVCHWDEDGKLTAWISTQTPFMVR
                     GILAEVLGLPLSKVRVLVDHMGGGFGAKQDLFQTEFLCALLAKETRRPVRLEFTRKET
                     FLGGRSRHPGTVWLKQGFKKDGTITARQARVTLNSGAYGSHGPGVTAVGTTALTSLYR
                     CENVHLEGRCVYTNTPIAGAYRGYGVVQTYYALDIQMDEAAEKLGIDPAELKLRNAVR
                     EGDLAPSGHAIVGHGLEDCIRRGMEETNWKRIRGQGANSFSRTQKPNSHPDPEFIRRG
                     WGMGCEMHGSSAYPGIKEQGNATVRMNEDGTVQLFTGTTGLGTGAHTALAQIVAEELG
                     VRFEYVSVVHGDTDVVPWDIGAFASHTTYMGGRAAQMAAAEVKTKLLESAAEALEAAT
                     ADLEVKNGVISVKGSDRHLSVRDAVSPKRGIPSAHLIASATYKPTKSYSFAAHFVEVQ
                     VDVQTGQIDVLQVIPVHEIGKVIHPVAAAGQIEGGIQQGIGHTLTEDHAVDPLTGRSL
                     NAGFVDYKMPLSMDMPPIRTILLETAPDPGGPFGAKGVGEDPIIAIGPAIANAVYDAT
                     GVRFRHYPITPEQVLEGLRKK"
     gene            5920..6882
                     /locus_tag="VIG70_03570"
     CDS             5920..6882
                     /locus_tag="VIG70_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8249659.1"
                     /translation="MNPVPVTILTGYLGAGKTTLLNYILTEKHGHRIAVIENEFGDIN
                     IDSELVLSSDEEIFELTNGCVCCVATARADLLRILEKLLARREKYDHILVETSGAADP
                     TPVAQTFFLDSLAQRLQLDAIVTMVDARHAAAHLDHHAVDQIVIADRIILNKVDLVAE
                     RELGELEAQVRRLNATAPILRSTHARVDLENILGIGAFSSQRGALALDVHEQSHDPDL
                     ESESFVFSQGFDRPRFQRYIRELLQERGDDIYRLKGIVAFADEPRRYVLQAVHRISDL
                     KPADAWGEEKPASRLVLIGRALDRTALEAGLSGCLAAASPASSP"
     gene            complement(6841..>7638)
                     /locus_tag="VIG70_03575"
     CDS             complement(6841..>7638)
                     /locus_tag="VIG70_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249660.1"
                     /translation="LSVWSSFAPIGFALGQWAAAYAGDPPLAVVGVSHAAILVLAAVA
                     LHFSIDLQVLQERRPSSGEALRHPPALWTALAFGGVCGVLLASVALAPIVLANQSRLS
                     VAHVASLTALAALPAIVGRIAPGWLLERGFAAFTIFTAASALAAISLAAALMAPLPLA
                     LALALFCAFQILAGALPGLLSAMLPHVAPAPAQLGTVSGMCSQSVNIGNLLGPPLALA
                     VYAAAGTGAAVVLLLAILGGSVAAIARLEVFRRDLRGRSGGSSGQAA"
BASE COUNT         1314 a         2583 c         2628 g         1113 t
ORIGIN      
        1 ccgatgttgt tggcgcccgg aatgtcgccg gccgccacca ccgcgaccac gccggaagac
       61 cggcgtaccg gctcgaggtc cagcgaacga atccgcccgt gggcgcattc cgacatgccg
      121 atggctgcgt agagcgtccc cggcggctcg ggcaagtcgt cgatgtagcg cgcctcgccc
      181 gcgacgtgca gcccggcgct gtcgtgcgcc agcgcttcgt gcacgccgcc gcggccggga
      241 cgctcggctt tcatggctgc atctcgagca gcgcgcgcgg gccgaccgtt gcgtggaact
      301 tgcgcagcag cccttgcgcg gcggcgagcc gataccagct cgaagcacgc atatcggaga
      361 tcggcttgaa gtcctcggcc agggccgagc aggcggcctc cacgccctgg ggccacattt
      421 tttcggcgcg gggggcgcgc gcaggggtgg ccgccatgcc gccgaagccg aagcgcacct
      481 cgtttccact cacgtgaaat gcggcgcaga cggcggagat gtcctggtcg aagcgcttgg
      541 agagcttgta cacggcgaag cgtgccgctc gcttcagttt cgggatgcgg attgcgcgga
      601 tgaactcgcc cggctgcagc acgtttttcc ggtagccggt gtagaagtca tccagggcga
      661 cggtcctttg ctgctgcccg cgctgcagcg ctacgctcgc tcccagagcg agcagcaccg
      721 gcatcgtgtc gccgatcggc gagccgttgg cgatgttgcc gcccagcgtc cctgtcgcgc
      781 gcacctgcgc cgcgccgagg cggcgcacga gctcgccgat gtccgggtga atgccggcga
      841 gcgccgcgta ggcgtcttca tacgtcacgg cggcgccgat ctctacgcag tcctcttctt
      901 cctcgagaac cctcagctcg gcaacccgtc ccacgtaggc aacagggtcg agatcgcggt
      961 gctgcttggt gatccacagc ccgacgtcgg tgccgccggc caggatcgtc gcctgcggat
     1021 gctcgaggag gaacgccgcg agctccgcga cgctcgccgg cgcgaagaag ttcatgacgc
     1081 gaaaagaacc tcccggcagg cgcgccaggc gcgagagctg cgcctggttc tcggcgagat
     1141 cgcgcttcgg gtccgcgagg gcccgtgact gctcgcacgc ctcgaggatc gggcggtagc
     1201 cggtgcagcg gcagaggttg ccggcgatcg cgtcggcggg cgaagcgcca tcggcccgct
     1261 tgtagtgcgc gtacatcgcc atgacgaatc caggcgtgca gaagccgcac tgggagccgt
     1321 ggcagtcgac catcgcgcgc tgcaccgggt gctcgccctt caggccttcg acggtgacga
     1381 cctctttgcc gtcgaccgcg ccgaggcaca ggatgcagct gttgacgggc aagtactgtg
     1441 tcttcccgtc ggcatcgagc tgggcaagta cgaccgtgca ggcaccgcag tcgccctcgg
     1501 cgcagccctc cttggtgccg acgaggccgc ggcgctcgcg cagccaggtg aggagggtgc
     1561 gcgtggggtc ctcgccggag agcgacagcg cttcgccgtt caggcaaaag tcgagcgagg
     1621 tccgcacctg gggatattgt caaccaaatc tcggccggat tgttgacaat ttcgagggtc
     1681 gcgcatagcc tcgacgcgtc atgccccgcg ccgccacccg caacggccgt gccgaggaac
     1741 gggtgttcaa cgcgatcctc gacgccgtgc tcgaccatcg cctcgcgccc ggcaccaagc
     1801 tgaaggagcg cgagctcgcc gagatcttcg gcgcgagccg cgcggcggtg cgcgccgcgc
     1861 tcacgcgcct cggccacagc ctgctggtcg agctgcgccc gaaccgcggc gcggtcatcg
     1921 ccaatccctc ggtcgccgag acgcgcgaga tgttcgaggc gcggcgcgtg atcgaatcgg
     1981 cgatcgtgca gcgcctcgcc cgcgcgctga gcgcaaagca gatcgccgag ttgcgcgctt
     2041 tcgtcgccga ggaaagccgt gcctaccgca aaggcgacca caaagcaggc cagcgcctgt
     2101 cgatccgctt ccacaaggtg ctgtccgagc tcgccggcaa ccgcacgctc gaccggttca
     2161 tggagcacct catctgccgc accccgttgc tcgcgctcgc ccgcaccggc cgcatcgcgt
     2221 actgcggcgc ggacgagcac cgcgaggtgg tcgaggcgct ggcgcgccgc gacgcggccg
     2281 ccgcggcgcg ctgcatgaca caacatctga acaacgtcga aggcgagctc gtcgccgagc
     2341 ccgagcccaa gccggaatcc agtctggccg cggcgctcac cgcatgaggc ggttcgagta
     2401 cttcgagccc gcgacgctcg gcgaggcgac ccagatcctc tcggctggag gtcacgtgct
     2461 tgcgggcggg accgacctgc tggtcgagat gaaagagcag ttgcggcggg tcgatcgcgt
     2521 ggtcaacatc aagaagatcc ccggcatggc cgggcttacc tacgacgaac gcgaagggct
     2581 gcggatcggc gcgctggtca ccgcgcgcga ggtcgaactt tcttcaatcg tcaaggaaaa
     2641 gtattccgga ttggtccagg ctgtgcgcga gctgggctcg atccaggtgc gcaaccgcgc
     2701 caccatcgtc ggcaacgtct gccgcgcctc gccgtcggcg gatacgctgc cgccgctcat
     2761 cgccgacgcg gcattggcag agatccacgg ccgaaccggc acgcgcaagg tcttgctgga
     2821 agaattcttc accggtccgg gcaagacagt gctcggcgca gacgagctca tgaccgcgat
     2881 cgtgctgccg ccgccggcgc cgcgcaccgg caaggtgtac atcaagcacg gccggcgcaa
     2941 ggcaatggag ctcgccacgg tcggcgtcgc ggtctcgctt accttgaacg cggaagtcag
     3001 ggtggtattg ggcgccgtgg cgccgacgcc gatccgcgcg aggaaagccg aggaagttct
     3061 tcgagggaga aaactgaacg aagaattgat tgaaaaggcg gcgcaggcgg cggcggacga
     3121 gtcgcgtccg atcagcaatg tgcgcgcgag cgccggctat cggcgcgaca tggtgcgcgt
     3181 gctcacgcgg cgcgccctcg agcaggcaat gcgatgctgc tgaagctcaa gatcaacggc
     3241 gaggcgcgcg agcttgcggt cgagccgtac cgcagcctgc tcgacgtgct gcgcaacgag
     3301 gccgggctca ccggcacgaa gaagggctgc gacgtcggcg actgcggcgc ctgcaccgtg
     3361 atcgtcgacg gcaagccggt caattcctgc ctgatgctgg gcgtggaggc gcaaggctgc
     3421 gaggtgctca cgatcgaagg gctgcggcat ccgctgcagg aaagcttcat ccggcacggt
     3481 ggcgcgcagt gcggcttctg cacgccaggc atgatcatga tggcgaaggc gctgctcgac
     3541 gagaatccgc ggccgagcga ggaggaagtg cgcttcgcga tctcgggcaa catttgccgc
     3601 tgcaccggct ataccaagat tgtggaagcg attcttcatg tcagtcatcg gcacgagcgt
     3661 taggcgtccg gatgtgccgg gcaaggtcac cggcgccgcg cgctacgccg gcgatctcgt
     3721 gctgtccggc atgctgcacg cgaagatcaa gcgctgcgcg atcgcccacg cgaacatcgc
     3781 gcgcatcgac accgcaagag cgctggccta tcccggggta aaggcggtgc tcacgcacct
     3841 gaacgtgccc cgcgtgctgc actacggctc gccgcacccg cgctcggcgt cgcttgcgcg
     3901 cgatcagtac atcctcgaca gcaaggttcg ctattggggc gagggcgtgg ccgcggtcgc
     3961 cgccgcgagc gaggagatcg ccgaggaagc gctcgacctg atcgaggtcg agtaccagga
     4021 gcttcccgcg gcaatcagcg tcgagcaggc gctcgcggct gatgcgccct cgattcatgg
     4081 gacgaaaagc aatctcgtca ccgatccggt gctcgtcaag cgcggcgaca tcgatcgcgg
     4141 gttcgcggaa gcggacctgg tcgtcgaggg cacctacgag atgggccgcc cgacgccggc
     4201 gtacatggag ccgaacgtct gcgtctgcca ctgggatgag gacggcaagc tcacggcctg
     4261 gatctcgacc cagacgccgt tcatggtgcg cggcatcctc gccgaggtgc tgggccttcc
     4321 gctctccaag gtgcgcgtgc tggtcgacca catgggcgga ggcttcggcg cgaagcagga
     4381 tctcttccag accgagttcc tgtgcgcgct gcttgcgaaa gaaacgcggc ggccggtgcg
     4441 gctggaattc acgcgcaagg aaaccttcct cggcggccgc tcgcgccatc cgggaaccgt
     4501 gtggctgaag cagggcttca aaaaggacgg cacgatcacc gcgcgccagg cgcgcgtcac
     4561 cttgaattcc ggcgcctacg gctcgcacgg ccccggcgtg accgcggtcg gcacgactgc
     4621 gctcacctcg ctttaccgct gcgagaacgt gcacctcgag gggcgctgcg tgtacaccaa
     4681 cacgccgatc gccggcgcct atcgcggcta cggcgtggtg cagacctact acgcgctcga
     4741 catccagatg gacgaggccg cggagaagct cggcatcgat ccggcggagc tcaagctgcg
     4801 caacgcggtg cgtgagggcg acctcgcgcc ttcgggccat gcgatcgtgg gtcacgggct
     4861 ggaagactgc attcggcgcg gcatggagga gacgaactgg aagcgaattc ggggtcaggg
     4921 tgcgaattcg ttttcgcgta cgcaaaaacc gaattcgcac cctgaccccg aattcatccg
     4981 tcgcggctgg ggcatgggtt gcgagatgca cggctcgagc gcctatccgg gcatcaagga
     5041 gcagggcaac gccacggtgc gcatgaacga ggatggcacg gtgcagctct tcaccggcac
     5101 cacgggtctt ggaacgggag cgcataccgc gcttgcgcag atcgtcgccg aggagctagg
     5161 cgtgcgtttt gaatacgtgt cggtggtgca cggcgatacc gatgtcgtgc cgtgggacat
     5221 cggcgcgttc gcgagccaca cgacctacat gggcgggcgc gccgcgcaga tggccgcggc
     5281 ggaagtgaag acgaagctgc tcgagtccgc ggccgaggcc ctggaagccg cgaccgcgga
     5341 tctcgaagtc aagaacggag tgatctcggt caaaggctcg gatcgccacc tgagcgtgcg
     5401 cgacgcggtg agccccaagc gcggcatccc gtcggcgcat ctcatcgctt cggcgaccta
     5461 caagccgacc aagtcgtatt cgttcgcggc gcatttcgtg gaggtgcagg tcgacgtgca
     5521 gaccgggcag atcgacgtgc tgcaggtgat cccggtgcac gagatcggca aggtgatcca
     5581 cccggtggcg gcggcggggc agatcgaggg cgggatccag cagggcatcg gccacacgct
     5641 caccgaggac catgcggtcg acccgttgac cgggcgctcg ctcaacgcgg gcttcgtcga
     5701 ctacaagatg ccgctctcca tggacatgcc gccgatccgc accatcctgc tcgagaccgc
     5761 gcccgatccg ggcgggccgt tcggcgccaa gggcgtgggc gaggatccga tcatcgccat
     5821 cggcccggcg atcgccaacg cggtctacga cgcgaccggc gtgaggttca ggcactaccc
     5881 gatcacgccg gagcaggtcc tggaagggct gcgaaaaaag tgaacccggt tcccgtcacc
     5941 atcctgaccg gctacctggg cgcgggcaag accacgctgc tcaattacat cctcaccgag
     6001 aagcacggcc accgcatcgc ggtgatcgag aacgagttcg gcgacatcaa catcgattcg
     6061 gagctggtgc tgtcctcgga cgaggagatc ttcgagctca ccaacggctg cgtgtgctgc
     6121 gtcgcaaccg cacgcgccga cctgctgcgc attctcgaga agctgctcgc gcggcgcgag
     6181 aagtacgatc acatcctcgt cgagaccagc ggcgcggccg acccgacgcc ggtggcgcag
     6241 accttcttcc tcgattccct ggcgcagcgg ctccagctcg acgcgatcgt caccatggtc
     6301 gacgcaagac acgccgccgc gcacctcgac caccacgcgg tcgaccagat cgtgatcgcc
     6361 gaccgcatca tcctgaacaa ggtcgacctc gtcgccgagc gcgagctagg cgagctcgag
     6421 gcgcaggtgc gccggctcaa cgccaccgcg ccgatcctgc gctcgacgca cgccagggtc
     6481 gacctcgaga acatcctcgg catcggcgcc ttcagctcgc agcggggggc gctcgcgctc
     6541 gacgtgcacg agcagtctca cgaccccgat ctcgagtcgg agtcgttcgt tttttcgcag
     6601 ggattcgacc ggccgcggtt ccagcgatac atccgggaac tgctccaaga gagaggcgac
     6661 gacatctatc gcctgaaggg catcgtcgca ttcgcggacg agccgcgccg ctacgtgctg
     6721 caggcggtgc accgcatcag cgaccttaag cccgccgacg cgtggggcga ggaaaagccg
     6781 gcgagccggc tggtgctcat cgggcgcgcg ctcgatcgca ccgcgctcga agccggcctg
     6841 tcaggctgcc tggccgctgc ttcccccgct tcttcccctt aggtcgcgcc ggaagacctc
     6901 gagccgcgcg atcgcggcga ccgagccgcc gagaatcgcc agcagcagca cgaccgcagc
     6961 gcccgtgccg gcggcggcat acaccgcgag cgcgaggggc gggcccagca ggttgccgat
     7021 gttcacgctc tgcgagcaca tccccgatac cgtgccgagc tgcgccggtg ccggcgcgac
     7081 gtgcggcagc atcgccgaca acagcccggg aagcgcgccg gccaggatct ggaacgcgca
     7141 gaacagcgcg agcgccagtg cgagcggcag cggcgccatc aacgccgccg cgaggctgat
     7201 ggcagcgagc gcgctcgccg cggtgaagat cgtgaacgcg gcaaagccgc gctcgagcag
     7261 ccagcccggc gcgatccgcc cgacgatcgc cggcagcgcc gccagcgcgg tgaggctggc
     7321 gacgtgcgcc accgaaagcc gggattgatt ggcgagaacg atcggcgcca gcgccaccga
     7381 cgcgagcagc accccgcaga ccccgccgaa ggcgagcgcg gtccatagcg ccggcgggtg
     7441 gcgcagcgct tcgccgctcg aagggcgtct ttcctgcagg acctgcaagt cgattgaaaa
     7501 atggagcgcg acagcagcca ggaccagaat cgcggcgtgc gacacgccca cgacggccag
     7561 cggcggatcg ccggcgtacg cggccgccca ttgcccgagc gcgaagccga tcggcgcgaa
     7621 gctcgaccac accgagag
//
LOCUS       DATBSZ010000056         9237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_416862, whole genome shotgun sequence.
ACCESSION   DATBSZ010000056 DATBSZ010000000
VERSION     DATBSZ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416862"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..916)
                     /locus_tag="VIG70_03580"
     CDS             complement(14..916)
                     /locus_tag="VIG70_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8249661.1"
                     /translation="MDPRRRALLAFLASAAALRPALAQDRIMTKQIPSTREPLPIIGV
                     GTWQTFDVGADSAARTPLREVLKLMTQSGRGVVDSSPMYGTSESVAGDLVAELGTREQ
                     LFMATKVWTSGRDAGIRQMETSFKRLRVERMDLMQVHNLVDVDTHTKTLQDWQAKKRV
                     RYIGITHYTSSAYAEVERQMKKAQYDFLQINYSLGERESEKRILGLAKDLSMAVIANR
                     PFAEGALFRRTKGKPLPPWSAELGIESWAQYFLKWIVSHPAVTCAIPGTGNPKHMTDN
                     LGAGRGALPDEAGRKRMAAYFDGL"
     gene            960..1661
                     /locus_tag="VIG70_03585"
     CDS             960..1661
                     /locus_tag="VIG70_03585"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY8249662.1"
                     /translation="MKRRAFILAMTAAAVVPPAFAQGKPMKIGVIGSGRIGSTIGGLW
                     VKAGHEVMFSDRDAEQVKRAIEGLGPRARAGSVPEAAAFGDAILIAVPYAALPAIQQQ
                     VGAQLKGKVVIDPNNPVPSRDGDMAVSAREKGAGVSSAALLPGARIVRSFNSWGYGTM
                     AREANRAAPRMALPVAADDPAALKLGMQLVSDAGFDPVAAGSLAASKAFDLGSSVSGR
                     VLTAPEMRQALQLKP"
     gene            1709..2899
                     /locus_tag="VIG70_03590"
     CDS             1709..2899
                     /locus_tag="VIG70_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8249663.1"
                     /translation="MLWSFAYFFCLLAGYYILRPLRDEMGVAGGVRNLQWLFTATFLV
                     MLAAVPLYGWLVARVPRRRFIPLVYHFFVLNLALFWVLLALDVERETVARVFFVWISV
                     FVLFAVSVFWSFMADVWSSAQGKRLYGFIAAGGSAGALAGPALTIGLAGTIGAVNLLI
                     VAALLLEAAVLCARKLEVEQKGSSERQNAVGGGAIDGILMVLRSPYIGGITLWVGLLS
                     VAATFLYFEQAAIVAAASDDPAVRTRIFAGVDLAVGLLTIAVQFFATGKLIARFGVGA
                     ALALLPVVFAVGFAVLAVAPVLAVVIAFQALQRTANFAISNPAREVLFTVVARDEKYK
                     AKNVIDIVAVRGADAAGGWLFTALRTLGMEVRSIPLVAIGIAAACLVIAVALGRAHER
                     RARL"
     gene            2928..3524
                     /locus_tag="VIG70_03595"
     CDS             2928..3524
                     /locus_tag="VIG70_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY8249664.1"
                     /translation="MGTGGWIVLAVIVAVAIWAIMIYNGLVTLRNRFKNAFSQIDVQL
                     KRRYDLIPNLVETVKGYMAHERGTLEAVVKARGAAVSAAQAAAAAPGDPGAMRGLAQA
                     EGALGGALGRLLAVFEQYPDLKANQNVLGLQEELASTENKVAFARQAFNDSVMEYNTK
                     RESFPENIFAGFFRFEAAELLQATDSAEERKAPKVSFT"
     gene            3526..5376
                     /locus_tag="VIG70_03600"
     CDS             3526..5376
                     /locus_tag="VIG70_03600"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY8249665.1"
                     /translation="MDFFEAQERSRRNSRWLILWYAVAVLCVVASYSAVAALAGAPPT
                     LYLAVGGAVGVFILAVSAYRMWQLAAGGAAIAHLMGARYVEPGKCAPAERRLLNVVEE
                     MAIASGISVPPVYLLDFDDAINALVAGYSPNEAVIIVTKGMVQRLSRDELQGVMGHEF
                     SHILNGDMALNLRLVGVLAGLTWMADAAERLILETARRERGLPREERGAGALAALGAG
                     LIAFIGFPGTFAADAIKGAISRERELLADAASVQFTRNPDGIAGALDSILALRAHTAV
                     RAVQCEPFSHMFFAPAVWRWWSFPTHPPILERIRRAHPRFRRDEYRERRHGIRREVAV
                     IDGSGSVVKNLRMGAALAMVASVGRPAVEHVDYAARLLARLPSRLREALHRADEAEHA
                     MFALALEPDEAAREAELQALAAKRGAESTAKVLELHVYVGGLARNHMLTLADLAVPAI
                     KGQRQKARDAFLADLAAMVESDRRVTLREFVLLTLLRQRLREGAGQPIRTQFRRIEEV
                     APDAHLVLSLVSLDHASFEKGAAVLKLGWTAPIGRDALSTQKVGESLERLRHLSPFAK
                     PGILKACFEAAAADGVFRLAEAELVRMVAATLDCPLPPVLAAQDPQALPA"
     gene            5373..7280
                     /locus_tag="VIG70_03605"
     CDS             5373..7280
                     /locus_tag="VIG70_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY8249666.1"
                     /translation="MTFFEHQSLARRNTRVMVLLFALAVIAVVVSVDLVLGALYVWSS
                     DQLFTLTAVPAGIYIGGAVATAALILLVSLYNVARLAEGGVAVARMVGARRVPPDTAD
                     ALERRLRNVVEEMAIAAGVRVPEVYVMDNERAINAFAAGWEVSGAVVAVTRGTLETLT
                     RDELQGVVAHEFSHILNGDMRLNVRMLGVLAGIVFIGSIGEFLMRSMRGSRDAKGGLA
                     LAGLAIFIIGYLGLVFARLIKSAVARQREFLADASSVQFTRNPDGIAGALDQIRTSGA
                     GTLIANRFAEEMSHMYFGAAVKVWLGGVFDTHPPLEERIRRVHPGFQPAEYRARRETA
                     ASTAREAADVKPTGRRLSDLATAWGRSPEQSAALVGTLDAAKIDYAARLLNALPASLQ
                     ESFRNADAAGAFMIALLLAEPEEAMGRQLEALGKTPLAERARAVAPLARHLGTALHLP
                     LIDLALPAVKSAPEETRKELLAALEAVINADRRVSLHEFVVLALVREQLAPRARAPQT
                     RQISELKAEAATVLALVAYAGTRVDAKGARDAALQSTIKAGAATMGIAESLVSPASLS
                     LQSASAALEALRSLAPMQKGVLIKGLFAAVTVDGTIRVAEAELMRLVGAVLDCPLPPL
                     LDSLDPATLAA"
     gene            complement(7242..8255)
                     /locus_tag="VIG70_03610"
     CDS             complement(7242..8255)
                     /locus_tag="VIG70_03610"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HEY8249667.1"
                     /translation="MVDLVPPLAIAFALRRNARLLAGVLVLAALSGCASLFPQTRELA
                     KGLPPGLPEKVELTEVPFFPQLEYQCGPAALATALSAVGRKVTPEELVPQVYIPDRKG
                     SLQVEMLAAARRHGMVSYALAPRFEDLLREVAAGTPVIVLQNLGLFSATWHYAVAVGY
                     DWGPGTLVLRSGTLERDVVPFAAHEVVWMRSGYWAMVAVPPDRIPVTAEEKSWLNAIA
                     AFERAGDARGARVAYQKFLGRWPENVNAYVGLANSHHALGELAQAAEVLREAARRDPE
                     SVVVLNNLAQTLSDLGRNEEALPVIERASAAGGPFAAAVQKTRETILDRLRGQRGRVE
                     RVQ"
     gene            complement(8231..8590)
                     /locus_tag="VIG70_03615"
     CDS             complement(8231..8590)
                     /locus_tag="VIG70_03615"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEY8249668.1"
                     /translation="MKSVWAKTLCRLLVVLMVWSPMQYAQAGMIGTDQVASAASQADR
                     NSVLQFLDRAEVARQMQSMGVDVATVKDRVAAMTDQEVQSIAGKIQSMPAGASDAGVI
                     ILILVVAAAVWWIWFRR"
     gene            8711..>9237
                     /locus_tag="VIG70_03620"
     CDS             8711..>9237
                     /locus_tag="VIG70_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8249669.1"
                     /translation="MQESLRVVEIQRLEAALRGALAGEVRFDKGMRAAYAADASNYRQ
                     VPIGVVLPRSAEDIVETMRLCASHGAPVLARGGATSLNGQAVNVAVVIDCSKYLGRVL
                     SIDAQSRLARVEPGVVCDALRDAAEAHGLTFAPDPATHSRCTLGGMIGNNSCGPHSVM
                     AGTTVDNIERLEVLT"
BASE COUNT         1312 a         3144 c         3274 g         1507 t
ORIGIN      
        1 cttgcggcgg atctcagagg ccatcgaagt aggcggccat gcgctttctt cccgcttcgt
       61 cgggcagggc gccacggccg gcgcccaggt tgtcggtcat gtgcttcggg ttgccggtgc
      121 cggggatggc gcaggtcacc gccgggtgcg acacgatcca cttgaggaag tactgcgccc
      181 agctttcgat gccgagctcg gcggaccagg gcggcagggg cttgcccttt gtgcgccgga
      241 acagggcgcc ttcggcgaac gggcggttgg cgatcaccgc catgctcaag tccttggcta
      301 aacccaaaat tctcttctcg gactcgcgct cacccagtga gtagttgatc tgcaggaagt
      361 cgtactgcgc cttcttcatc tggcgctcga cctcggcata ggcggaggac gtgtagtgcg
      421 tgatgccgat gtagcgcacg cgcttcttcg cctgccagtc ctgcaaggtc ttcgtgtgcg
      481 tgtcgacgtc gaccaggttg tgcacctgca tgagatccat gcgctcgacg cgcaggcgct
      541 tgaaggaggt ttccatctgc cggatgccgg cgtcgcggcc gctggtccag accttggtgg
      601 ccatgaagag ctgctcgcgc gtgccgagct cggcgaccag gtcgccggcc acgctctccg
      661 acgtgccgta catcggagaa gagtcgacga cgccacgacc actctgagtc atcagtttca
      721 atacttcgcg cagcggggtg cgggcggcgc tgtcggcgcc gacgtcgaag gtttgccaag
      781 tgccgacgcc gatgatcggc agcggctcgc gcgtggatgg aatctgcttg gtcatgatcc
      841 tgtcctgtgc cagcgcgggt ctgagcgcgg cggcgctcgc taggaaggcg aggagcgcgc
      901 gccgtcgcgg gtccatgagt cgattctagc tgtgatagcc tgacccgaag gagacctcga
      961 tgaagcgacg cgctttcatt ctcgccatga cggcggccgc ggtggttccg cccgcgttcg
     1021 cgcagggcaa gccgatgaag atcggcgtga tcggctcggg acggatcggc tcgacgatcg
     1081 gcgggctgtg ggtgaaggcg ggccacgagg tcatgttttc cgaccgcgat gcggagcagg
     1141 tgaagcgcgc gatcgagggc ttgggaccgc gcgcacgagc cggcagcgtg ccggaagccg
     1201 cggcgttcgg cgacgcgatc ctgatcgcgg tgccctatgc ggcgctgccc gcgatccagc
     1261 agcaggtcgg cgcccagctc aagggcaagg tggtgatcga cccgaacaac ccggtgcctt
     1321 cgcgcgatgg cgacatggcg gtgtcggcgc gcgaaaaggg cgcgggcgta tcgagcgcgg
     1381 cgctgctgcc cggggcgcgc atcgtgcgca gcttcaattc gtggggctac gggaccatgg
     1441 cgcgcgaagc gaaccgcgcg gcgccgcgca tggcgttgcc ggtcgccgcc gatgatcccg
     1501 ccgcgctgaa gcttggcatg cagctggttt cggacgcggg cttcgacccg gtggctgccg
     1561 ggtcgctcgc cgcgtcgaag gccttcgacc tcgggtcatc ggtttcgggt cgtgtgctca
     1621 ccgcgcccga aatgcgtcag gcgctgcagc tcaaaccctg aaggttcttt ctcgagtcgt
     1681 tgcggtgcgg ccggaggaag tccgcgccat gctgtggtcg ttcgcgtact tcttctgcct
     1741 gctcgccggc tactacatcc tgcgcccgct gcgcgacgag atgggcgtgg cgggcggcgt
     1801 gcgcaacctg cagtggctct tcaccgcgac cttcctcgtc atgctcgccg cggtgccgct
     1861 ctacggctgg ctggtcgcgc gcgtgccgcg gcgccgcttc atcccgctcg tctaccactt
     1921 cttcgtcctc aacctggcgc tgttctgggt gctgctcgcg ctcgacgtcg agcgcgagac
     1981 ggtggcgcgc gtgttcttcg tctggatcag cgtgttcgtc ctgttcgcgg tctcggtgtt
     2041 ctggtcgttc atggccgatg tctggagctc cgcgcagggc aagcgcctct acggcttcat
     2101 cgccgcgggc ggcagcgcgg gggcgctcgc cgggcccgcg ctcaccatcg gcctcgccgg
     2161 cacgatcggc gcggtgaacc tgctgatcgt cgcggcgtta ctgctcgagg cggcggtgct
     2221 ctgcgcccgg aagctcgagg ttgaacaaaa gggttcctct gaaagacaga atgccgtcgg
     2281 cggcggcgcg atcgacggca tcctcatggt gctgcgctcg ccctacatcg gcggcatcac
     2341 gctctgggtc gggctgctgt cggtggccgc gaccttcctc tacttcgagc aggccgccat
     2401 cgtcgccgcc gcctccgacg accccgcggt gcgcacgcgc atcttcgccg gcgtcgacct
     2461 cgccgtcggc ctgctgacga tcgcggtcca gttcttcgcc accggcaagc tgatcgcgcg
     2521 cttcggcgtg ggcgcggccc tcgcgctgct gccagtcgtg ttcgcggtcg gcttcgcggt
     2581 gctcgccgtg gcgccggtgc tggcggtggt gatcgcgttc caggcgctgc agcgcaccgc
     2641 gaatttcgcg atctcgaatc ccgcgcgcga ggtgctgttc acggtcgtcg cgcgcgacga
     2701 gaagtacaag gccaagaacg tgatcgacat cgtcgcggtg cgcggcgccg acgccgcggg
     2761 cgggtggctg ttcaccgcgc tgcgcacgct cggcatggag gtgcgctcga tccccctggt
     2821 ggcgatcggg attgcggccg cctgcctcgt gatcgcggtg gcgctcggcc gcgcgcacga
     2881 gcgccgggct aggctatagt taatcagcag tcaaaacggg aggacgcatg ggcaccgggg
     2941 gatggattgt tctggcagtg atcgtggctg tggcgatctg ggccatcatg atctacaacg
     3001 ggctggtcac gctgcgcaac cggttcaaga acgcgttctc gcagatcgac gtccagctca
     3061 agcgccgcta cgacctgatt ccgaacctgg tcgagacggt gaaagggtac atggcgcacg
     3121 agcgcggcac gctcgaggcc gtggtcaagg cccgcggcgc cgcggtgagc gccgcgcagg
     3181 cggcggccgc cgcgccgggc gacccgggcg cgatgcgcgg cctcgcgcag gccgagggcg
     3241 cgctcggcgg ggcgctcggc cgcctcctgg cagtgttcga gcagtacccg gatctgaagg
     3301 cgaatcagaa cgtgctcggc ctgcaggagg agctcgccag caccgagaac aaggtcgcgt
     3361 tcgcccgcca ggcgttcaac gactcggtga tggaatacaa caccaagcgc gaatccttcc
     3421 cggagaacat cttcgcgggc ttcttccgct tcgaggccgc ggagctcctg caggcgaccg
     3481 actcggccga ggagcgcaag gcgccgaagg tctcgttcac gtagcttgga ttttttcgaa
     3541 gcgcaggagc gctcgcgccg caactcgcgc tggctcatcc tttggtacgc ggtcgcggtg
     3601 ctctgcgtcg tcgcgagcta cagcgcggtc gccgcgttgg cgggcgcccc gccaacgctc
     3661 tatctggcgg tcgggggcgc cgtaggcgtc ttcatcctgg cggtgtccgc ctaccggatg
     3721 tggcagctcg ccgccggggg ggcggcgatc gcgcacctga tgggggcgcg gtacgtcgag
     3781 ccgggcaagt gcgcgcccgc cgagcgcagg ctgctcaacg tcgtggagga gatggcgatc
     3841 gcctcgggga tctccgtgcc cccggtgtac ctgctcgact tcgacgacgc gatcaacgcc
     3901 ctggtcgccg gctactcgcc gaacgaggcg gtcatcatcg tgaccaaggg gatggtgcag
     3961 aggctttcgc gcgacgagct ccaaggggtg atgggtcacg agttcagcca catcctgaac
     4021 ggcgacatgg cgctcaacct gcggctcgtc ggggtcctcg cgggcctgac ctggatggcc
     4081 gacgccgccg agcgcctgat cctcgagacc gcccggcgcg agcgcggcct gccgcgcgag
     4141 gagcgcggcg cgggggcgct ggccgcgctc ggggcagggc tcatcgcctt catcggcttt
     4201 cccgggacct ttgccgccga cgccatcaag ggcgcgatct cccgcgagcg cgagctgctc
     4261 gcggacgcgg cgagcgtgca gttcacgcgc aaccccgacg gcatcgcggg cgcgctcgac
     4321 tcgatccttg ccctgcgcgc gcacactgcg gtgcgcgcgg tgcagtgcga gccgttctcg
     4381 cacatgttct tcgcgccggc agtctggcgc tggtggagct ttccgaccca cccgccgatc
     4441 ctcgagcgga tccgccgcgc ccatccgcgc ttccggcgcg acgagtatcg cgagcggcgc
     4501 cacgggattc gcagggaagt cgccgtcatc gatggctcgg gcagcgtggt gaaaaatctg
     4561 cgcatgggcg ccgcgctggc gatggtggcc tcggtcgggc ggcccgcggt cgagcacgtc
     4621 gactacgcgg cgcggctgct cgcaaggctg ccgtcgcgcc tgcgcgaggc gctgcaccgc
     4681 gccgatgagg ccgagcacgc gatgttcgcg ctggcgctcg agcccgacga ggcggcgcgc
     4741 gaggccgagc tgcaggcgct ggcggcgaag cgcggcgccg aatcgacggc gaaagtgctc
     4801 gagcttcacg tgtacgtcgg cggactcgcg cgcaaccaca tgctcacgct cgccgacctt
     4861 gcggtccccg cgatcaaggg gcagcggcag aaggcgcgcg acgcctttct cgcggacctc
     4921 gccgcgatgg tcgaatccga ccggcgcgtg accctgcggg agttcgtgct gctgaccctg
     4981 ctgcggcagc gcctgcgcga aggcgcgggc cagcccatcc gcacgcagtt ccgccgaatc
     5041 gaggaggtcg cgcccgacgc gcacctcgtg ctgtccctgg tttcactcga ccacgcctcc
     5101 ttcgaaaaag gcgctgccgt gctgaagctc ggctggacgg cgccgatcgg gcgcgacgcg
     5161 ctgagcacgc agaaggtcgg cgagtcgctc gagcgcctgc gccacctgtc gcccttcgcc
     5221 aagcccggca tcctgaaggc gtgcttcgaa gccgccgccg cggacggcgt gttccggctg
     5281 gccgaagccg agctggtgcg catggtggcg gcgacgctgg actgtccgct gcctcccgtg
     5341 ctggccgcgc aggacccgca ggcgctcccc gcgtgacctt cttcgagcac cagagcctcg
     5401 cgcggcgcaa caccagagtg atggtgctgc tttttgcgct tgcggtgatc gcggtcgtcg
     5461 tttcggtcga cctggtgctc ggcgcgctct atgtgtggag cagtgaccag ctcttcacgc
     5521 tgacagccgt gcccgccggg atctacatcg gcggcgcggt ggccaccgcc gcgctgatcc
     5581 tgcttgtcag cctgtacaac gtcgcgcggc ttgccgaggg cggcgtggcg gtcgcgcgca
     5641 tggtcggcgc tcggcgggtg ccgcccgaca ccgccgatgc gctcgagcgg cgcctgcgca
     5701 acgtggtcga ggaaatggcg atcgccgccg gcgtgcgcgt tcccgaggtg tacgtgatgg
     5761 acaacgagcg cgccatcaac gcgttcgccg cgggctggga agtgtcgggc gcggtggtgg
     5821 cggtgacgcg cggcacgctc gagacgctca cgcgcgacga gctgcagggc gtggtggcgc
     5881 acgagttcag ccacatcctg aacggcgaca tgcggctcaa cgtgcgcatg ctcggcgtgc
     5941 tcgcggggat cgtgttcatc ggctcgatcg gcgagttcct catgcgcagc atgcgcggct
     6001 cgcgcgacgc caagggcggc ctcgccctcg ccggcctcgc catcttcatc atcggctacc
     6061 tgggcctggt ctttgcgcgg ctcatcaagt cggcggtggc gcgccagcgc gagttcctcg
     6121 ccgacgcctc cagcgtccag ttcacgcgca atcccgacgg catcgcgggc gccctggacc
     6181 agatccgcac gtcgggcgcg ggcacgctga tcgccaaccg cttcgccgag gagatgtcgc
     6241 acatgtactt cggcgcggcg gtgaaggtgt ggctcggcgg cgtgttcgac acccatccgc
     6301 cactcgagga gcgcatccgc cgcgtgcatc cggggttcca gcctgccgag taccgggcca
     6361 ggcgggaaac cgcggcctcg acggcgaggg aagccgccga tgtgaagccg accggccgcc
     6421 gcctgagcga cctcgccacc gcgtggggcc gctcgcccga gcagagcgcg gcgctcgtcg
     6481 gcacgctcga tgcggccaag atcgactatg ccgctcgtct gctgaacgcg cttccggcgt
     6541 cgctgcagga aagcttccgc aacgccgacg ccgcgggcgc attcatgatc gcgctcctcc
     6601 tcgccgagcc cgaggaggcg atgggccggc agctcgaggc cctgggaaag acgccgctcg
     6661 cagagcgcgc ccgcgcggtg gcgccccttg cccggcacct cggcacggcg ctccaccttc
     6721 cgctgatcga cctcgcactt ccggcggtga agtctgcgcc ggaagagacg aggaaggagc
     6781 tgctcgcggc cctcgaggcg gtgatcaacg cggaccggcg ggtatcgctg cacgagttcg
     6841 tggtgctcgc gctcgtgcgc gagcagctcg cgcccagagc cagggcgccc cagacccggc
     6901 agatctccga gctgaaagcc gaggccgcga ccgtgcttgc gctggtcgcc tacgccggca
     6961 cgcgcgtcga cgcgaagggg gctcgagacg ccgcattgca gtcgacgatc aaggccggcg
     7021 ccgcgacgat ggggattgcg gaaagccttg tgtcgcccgc cagcctctcg ctccagtcgg
     7081 ccagcgccgc gctcgaagcg ctgcgcagcc ttgcgccgat gcagaaaggc gtgctgatca
     7141 agggactgtt cgccgcggtg accgtcgacg gcaccatccg ggtcgcggaa gccgagctga
     7201 tgcgcctggt cggcgcggtg ctcgactgcc ctctgccgcc gctactggac tcgctcgacc
     7261 cggccacgct ggccgcgtag ccgatccagg atcgtttccc gcgtcttctg cactgcggcg
     7321 gcgaacggac cgcctgcggc cgacgcgcgc tcgatgaccg gcagtgcttc ctcgttgcgg
     7381 ccgaggtccg acagcgtctg cgcgaggttg ttgagcacca cgaccgactc cgggtcgcgg
     7441 cgcgccgctt cgcgcagcac ttccgcggct tgcgccagct cgccgagcgc gtggtgcgag
     7501 ttcgcaaggc cgacgtacgc attcacgttc tccggccagc ggccgaggaa cttctggtag
     7561 gcgacgcgcg cgccgcgcgc atcgccggcg cgctcgaaag cggcgatcgc gttcagccag
     7621 ctcttctcct cggcggtgac gggaatgcga tcgggcggca ccgcgaccat cgcccagtag
     7681 ccgctgcgca tccagacgac ctcgtgcgcg gcgaacggca ccacgtcgcg ctccagcgtt
     7741 ccggagcgca gcaccagcgt tccgggtccc cagtcgtagc cgacggcgac ggcgtagtgc
     7801 cacgtggcgc tgaacaggcc gaggttctgc agcacgatca ctggcgtccc ggccgcgact
     7861 tcgcgcagca ggtcctcgaa gcgcggcgcg agtgcgtacg acaccatgcc atggcgccgc
     7921 gctgccgcca gcatctcgac ctgcaggctg cccttgcgat ccgggatgta gacctgcggc
     7981 acgagctcct ccggcgtcac cttcctgccc acggcagaaa gcgccgtcgc cagcgccgct
     8041 gggccgcact ggtactcgag ctgcggaaag aaaggcactt cggtgagctc gaccttctcc
     8101 ggcaggcccg gcggcagccc cttcgccagc tccctcgtct gcgggaagag gctggcgcag
     8161 cccgagagcg ccgccaaaac gagaacgccc gccaggaggc gggcgtttct tcgtagagcg
     8221 aaggcgatgg ctagcggcgg aaccagatcc accacacggc ggccgcgacc accaggatca
     8281 ggatgatgac gccggcgtcg ctggcgccgg cgggcatgct ctggatcttg ccggcgatcg
     8341 actggacttc ctgatcggtc atggccgcga ctcggtcctt gaccgttgcg acgtcgacgc
     8401 ccatcgactg catctgcctc gcgacttcgg cgcggtcgag gaattgcagt acggagttgc
     8461 ggtcggcctg cgaagcggcg gaggcgacct ggtcggtgcc gatcattccg gcctgcgcgt
     8521 actgcatcgg cgaccagacc atcagcacca ccaacaagcg gcagagcgtc tttgcccaaa
     8581 ctgatttcat gttgtgaagt cctcgggtga tagggggaaa catcttgaca tgacgaccgt
     8641 caagcggttc cggtcgtctg tgaaccagcg tagtccgcgc ctttgatgaa gtcaagaaaa
     8701 cgcggggcat atgcaagaat cactacgggt ggtcgagatc caacgactgg aagcggcgtt
     8761 gcgcggcgca ttggcgggcg aagtgcgctt cgacaaaggc atgcgggctg cgtacgccgc
     8821 cgacgcctcg aactaccgcc aggtaccgat cggcgtcgtg ctgccgcgca gcgccgagga
     8881 catcgtcgaa accatgcggt tgtgcgcgag ccacggcgcg ccggtgcttg cgcgcggcgg
     8941 agcgacctcg ctcaacggcc aggcggtcaa cgtcgcggtg gtgatcgact gctcgaagta
     9001 cctcggccgg gtgctgtcga tcgacgcgca gtcgcggctt gcgcgcgtgg agcccggggt
     9061 ggtgtgcgac gcgctgcgcg atgccgccga agcgcacggg ctcaccttcg cgccggaccc
     9121 ggcgacgcat tcgcgctgca ccctcggcgg catgatcggc aacaactcct gcgggccgca
     9181 ttcggtgatg gcgggcacca cggtggacaa catcgagcgc ctcgaggtgc tcaccta
//
LOCUS       DATBSZ010000057         7778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1099849, whole genome shotgun sequence.
ACCESSION   DATBSZ010000057 DATBSZ010000000
VERSION     DATBSZ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099849"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VIG70_03625"
     CDS             <1..500
                     /locus_tag="VIG70_03625"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEY8249670.1"
                     /translation="SLIVFSCLMEGLFFYVGFAQILALGRQNKMTGAAEQYMYILRDE
                     SMHCNFGIDLVNTIKLENPHLWTAGFREELTGIFRKAVELEYAYAEDTMPRGVLGMNA
                     PMFKEYLRFIANRRCQQIGLDALYPGAANPFPWMSEMIDLKKERNFFETRVIEYQTGG
                     ALSWD"
     gene            528..827
                     /locus_tag="VIG70_03630"
     CDS             528..827
                     /locus_tag="VIG70_03630"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEY8249671.1"
                     /translation="MLEEFDTMRITACKAEDDYKLWLRFEDGLEGRVFLGNLLEIGAF
                     QLWRDVREFEKVSVDPETATVTWEGGIRLDADILYHDVKANQIQHAGGEVPPAVG"
     gene            complement(859..1257)
                     /locus_tag="VIG70_03635"
     CDS             complement(859..1257)
                     /locus_tag="VIG70_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249672.1"
                     /translation="MTKPVRSSFGPTGASATASFTARLAGYGREPVGNGHAAAGFSAV
                     LAPGVAAVDGVVAGAIVSAELAGKAACIAFFFFPAFFFFLADFFLPAAFFLPAVLSDF
                     FFFFDAFFFFFFLPPFFAFFFAILDLLEGC"
     gene            1547..2239
                     /locus_tag="VIG70_03640"
     CDS             1547..2239
                     /locus_tag="VIG70_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY8249673.1"
                     /translation="MNETGITRHLSCALAALRDLFALAGVAAIFVYFVLPLRGPIEQV
                     LPTLPAALIDPLAVMASAAEGPTTVVETPQEREQRAVAEFIAKRYRVAEDAAAGFVDI
                     AYRAGEQHRVDPLLILAVMAIESRYNPVAESVVGARGLMQVIPRYHPEKLEPHGGEQA
                     LLVPEVNIVVGAQILREYTRRFGDLEAALQMYNGALDEPTSRYANKVFAEKAQLDALR
                     VKARKQQIAQAV"
     gene            complement(2202..3356)
                     /locus_tag="VIG70_03645"
     CDS             complement(2202..3356)
                     /locus_tag="VIG70_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8249674.1"
                     /translation="MRTARRAAEIEPFEVMDVLSRAQALERAGRRVVHMEIGEPDFTA
                     PAPVVEAGIRALRDGRTAYTATLGLASLREAIAGHYEKRFGARVSPGRIAITSGASGG
                     LLTVSALYVDPGDEILVPDPGYPGYRHFVRAFEGAAKILPVSAKSDFQPTLEMVRAAW
                     GPRTKGMLLGSPSNPTGTLIAAKELRAIADFIAGRGGVLLVDEIYQGLVYGMEPWTAF
                     GLPGEVVLINSFSKYFCMTGWRLGWVVLPQDALRGFEKLAQHLFISAPAAAQHAAVSA
                     FEPESLSVLEQRRGEFARRRDFLVPALKKAGLSIPAEPHGAFYVYADCGGDARRYARE
                     LLETEAVAATPGVDFGANGTERFVRFAYTRSMEDLEEAARRLARFAASAP"
     gene            3380..5674
                     /locus_tag="VIG70_03650"
     CDS             3380..5674
                     /locus_tag="VIG70_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY8249675.1"
                     /translation="MDEQLRRNALDYHRRHPPGKVSVAPTKQLTNQRDLALAYSPGVA
                     AACDEIKRDPAQAVHYTARANLIAVVTNGTAVLGLGAIGPLAAKPVMEGKSVLFKKFA
                     GIDCFDIEIDERDPHKLIDIVAALEPTFGAINLEDIKAPECFIVERALRQRMKIPVFH
                     DDQHGTAITVGAAIFNGLKLLRKDIASVKLVASGAGAAALACLELLVKMGLRRENIVV
                     TDIKGVVYKGRREEMDPDKERFAVETRARTLDEVIAGADVFLGLSAGGVLKGAMVKKM
                     AERPLILALANPDPEISPDEAKAARPDCVIATGRSDFPNQVNNVLCFPFIFRGALDVG
                     ATTINTEMELAAAKAIAELAQAETSEQVALAYGIDTISFGPEYIIPKPFDPRLIARVA
                     PAVAKAAMDSGVATRPLASLDAYRESLSRFVYHSGVIMQPLFTAAKAAPRRVVYAEGE
                     DERVLRAAQVVVDEGLARPTLIGRPQVIEQRLERLGLRVRPGKEFDLVNPESDPRYRD
                     YWTTYHQLSERKGITPEYARVEMRRHNTLIGAMLMHKGEADGMLCGTFGMHAEHLRYI
                     DQVIGLRAGVRNYAAMNALMLAARNVFICDTHVTEDPGAEHLAEMTLLAADEVRRFGI
                     TPKVALLSASNFGSAQTASSRKMQRALELIRNLAPELEIDGEMHGDAALSEEIRLRVF
                     PRTRLRGEANLLVMPTLDAANISFNLLKTAAGGGVTIGPMLLGAARPVHILTPSATVR
                     RLVNMTALVVADVNAPRAQEQNLF"
     gene            5674..6861
                     /locus_tag="VIG70_03655"
     CDS             5674..6861
                     /locus_tag="VIG70_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8249676.1"
                     /translation="MEPASNVAPFPAGRTRARAGLVLTGGGARAAYQVGVVKAVRDIL
                     GNPTKNPFPILCGTSAGAINVATLAVFADNFSRGVANLLEVWEHMRCEHIYRTDAWNI
                     LRAGARWLSAMMLLSRHNPVSLLDNAPLREMLDKNLNFERIQGHIDSGALYAVCVTAS
                     GYTSGQSVSFFQGGSGIEGWERNQRIGAAVSIKLDYLLASAALPFIFPAVKVHREYFG
                     DGSMRQIAPVSPALHLGADRVLIVGTGRQHTDDARARSNTYPSLAQIAGHALNSIFLD
                     SLMVDIERLERINRTVKLIPPERLAESNVPLRAVKVLFITPSQPIERVASRFIHDLPR
                     TVRFVLRPTGALNRSGSNLASYLLFEESFCRALIDLGYQDTMAREAEVKDFFGPPEER
                     AYG"
     gene            6854..7102
                     /locus_tag="VIG70_03660"
     CDS             6854..7102
                     /locus_tag="VIG70_03660"
                     /inference="COORDINATES: protein motif:HMM:NF043076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HEY8249677.1"
                     /translation="MADDNPPQDPFEMFRRLWGPLGVPIPGMAMPTLDPQEVEKRIAD
                     LRSVEGWLTMNLNMVKLSIQGLEMQKATLQAMQPRKDK"
     gene            7122..>7778
                     /locus_tag="VIG70_03665"
     CDS             7122..>7778
                     /locus_tag="VIG70_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY8249678.1"
                     /translation="MAKAIRYHKQGGPEVLQLDDIPVGEPGQGQVRIRHTAIGVNFVD
                     TYQRSGLYPMQLPAVAGNEAAGVVDAVGPGVKDLKKGDHVAYTGLPGSYCDERIVPAD
                     RMVKIPKGISDEQAASMLLKGMTVHYLIHSTYKVKKGDMVLWHAAAGGVGLIAGQWLK
                     KLGAVTIGTVSSDEKAKLAKAHGYKHVINYSTENFVEKVKEITKGKKLPVVYDAVGKT
                     T"
BASE COUNT         1449 a         2579 c         2569 g         1181 t
ORIGIN      
        1 agagcctgat cgtgttctcg tgcctgatgg aggggctgtt cttctacgtc ggcttcgcgc
       61 agatcctggc gctcgggcgg cagaacaaga tgaccggcgc ggccgagcag tacatgtaca
      121 tcctgcgcga cgagtcgatg cactgcaatt tcggcatcga cctcgtgaac accatcaagc
      181 tggagaaccc gcacctgtgg acggccgggt tccgcgagga gctgaccggc atcttccgca
      241 aggcggtgga gctcgagtac gcctacgccg aggacacgat gccgcgcggc gtgctgggga
      301 tgaacgcgcc gatgttcaag gaatacctgc gcttcatcgc caaccggcgc tgccagcaga
      361 tcgggctcga cgcgctctac cccggggcgg cgaatccctt cccgtggatg agcgagatga
      421 tcgatctgaa aaaggaacgc aacttcttcg agacccgggt gatcgagtac cagaccggcg
      481 gtgcattgag ttgggactga cgtttaaaag caacgagggg gaaccacatg ctcgaggagt
      541 tcgatacgat gcgaataaca gcatgcaaag ccgaagatga ctacaagctc tggctgcgct
      601 tcgaagacgg cctggaaggc cgcgtgttcc tcggcaatct gctcgagatc ggcgccttcc
      661 agctgtggcg cgacgtgcgc gagttcgaga aggtgagcgt cgatcccgag acggcgaccg
      721 tgacgtggga gggaggcata cgcctcgacg cggatattct ttatcatgac gtgaaggcga
      781 accagattca gcatgccggc ggcgaagtgc cgccggcggt tggatagccc gggcttgctt
      841 gcccgagctg ccgtttcttc aacaaccttc aaggagatcg agaatggcga agaaaaaagc
      901 gaaaaaaggc ggcaagaaaa agaagaaaaa gaaagcatca aaaaagaaga agaagtcact
      961 gagaacagct ggtaagaaaa aggcggcagg caaaaagaaa tccgccaaga agaagaaaaa
     1021 ggcggggaag aagaaaaagg cgatacaagc cgccttaccg gccagctccg cggaaacgat
     1081 ggcgcccgca acgacgccat cgaccgctgc gactcccggc gcgagaaccg cgctgaatcc
     1141 agcggccgcg tggccgttcc ccaccggctc ccggccgtaa ccggcgagtc gagcggtgaa
     1201 cgaggcggtg gcggaagccc ccgtcggccc gaagctcgag cggaccggct tggtcattcg
     1261 gcccaagaac ggagacttag gccggtccgc gaggcttcct gaagcgactc cccaagggag
     1321 cgctttggga aactccatct taccggcctc gaccctccca tccaacatga aaaattatgt
     1381 cgaaccacgc gatttgacag gcctttatcg agtggtccgt atgtactaga agtgattgac
     1441 ttttaaaggg aattcgtcat attatgaggc ccgctgcggt gcaacaagga cgcttccggc
     1501 gcacaccccg gcagggcaca ctacggctcg aggcaggagg tccaagatga acgaaacggg
     1561 catcacgcgg cacctgtcgt gcgcgctggc tgcactcagg gacttattcg ccctggcggg
     1621 ggtggcggca atctttgtgt actttgtcct gcccctgcgc gggccgatcg agcaggtatt
     1681 gccgacccta cccgcggcgc tgatcgaccc gcttgccgtc atggcgagcg cagccgaggg
     1741 gccgaccacg gtggtcgaga ccccgcagga gcgcgagcag cgggcggtcg ccgagttcat
     1801 cgccaagcgc taccgcgtgg ccgaggacgc ggcggcgggc ttcgtcgaca tcgcctaccg
     1861 ggccggcgag cagcaccggg tcgacccgct cctgatcctc gcggtgatgg cgatcgagtc
     1921 gcgctacaac ccggtggccg aaagcgtcgt gggcgcgcgc ggtctgatgc aggtgatccc
     1981 gcgctaccac cccgaaaagc tcgagccgca cggcggggag caggcgctgc tcgtgcccga
     2041 ggtcaacatc gtggtcggcg cccagatcct gcgcgagtac acgcggcgct tcggcgacct
     2101 ggaagccgcg ctgcagatgt acaacggcgc gctggacgag ccgacgtccc ggtacgcgaa
     2161 caaggtattt gccgagaaag ctcagctgga cgcgctcagg gtcaaggcgc ggaagcagca
     2221 aatcgcgcaa gccgtctagc ggcctcctcg agatcctcca tgcttcgcgt gtaggcgaag
     2281 cgcacgaagc gctcggtgcc gttggcgccg aagtccacac ccggcgtcgc cgcgaccgct
     2341 tctgtttcga gcagctccct cgcgtagcgg cgggcgtcgc cgccgcagtc cgcgtataca
     2401 tagaatgcgc cgtgcggctc ggccgggatc gacagcccgg ccttcttgag cgcgggcacg
     2461 agaaagtccc gccgtctggc gaattcaccc cttctttgtt ccagaacaga gaggctttcc
     2521 ggttcgaagg cgctgaccgc cgcatgctgc gcggcggcgg gggcgctgat gaaaagatgc
     2581 tgcgcgagct tctcgaagcc gcgcagcgcg tcctgcggca gcacgaccca ccccaggcgc
     2641 cagccggtca tgcagaagta cttcgagaag ctgttgatca gcacgacctc gcccggcagg
     2701 ccgaacgcgg tccagggctc catgccgtac accagtccct ggtagatctc gtccacgagc
     2761 agaacgccgc cgcggcccgc gatgaagtcg gcaattgccc ttaattcctt tgcggcaatc
     2821 aaagttcctg tcgggttcga aggcgagccg agcagcatcc ccttggtgcg cggtccccag
     2881 gccgcgcgca ccatctcgag cgtcggctgg aaatcgctct tcgccgagac cggcaggatt
     2941 ttcgccgcgc cctcgaacgc gcgcacgaag tgccggtacc cgggatagcc ggggtcgggc
     3001 accaggatct cgtcgccggg gtcgacgtag agcgcactga ccgtcagcaa cccgcccgag
     3061 gcgcccgaag taatcgcaat tctgccggga gaaacgcgcg cgccgaatcg cttctcgtaa
     3121 tggccggcga tcgcttcgcg cagtgaagcc aggccaagcg tcgccgtgta ggcggtgcgg
     3181 ccgtcgcgca gcgcgcgaat tcccgcttcc accaccggcg cgggggcggt gaagtcgggc
     3241 tcgccgatct ccatgtgcac cacgcggcgg cccgcgcgct cgagcgcctg cgcgcgcgac
     3301 agcacgtcca tcacctcgaa gggctcgatt tcggcggcgc gccgcgccgt tcgcatggat
     3361 ctgattatag aatcgtcgcg tggacgagca gctgaggagg aacgccctcg actatcaccg
     3421 gcgccacccg cccggcaagg tctcggtggc gccgaccaag cagctcacca accagcgcga
     3481 cctcgcgctc gcctattcgc ccggcgtcgc ggcggcgtgc gacgagatca agcgcgatcc
     3541 ggcgcaagcg gtccactaca ccgcgcgcgc caacctgatc gcggtggtca ccaacggcac
     3601 cgcggtgctc ggcctgggcg cgatcgggcc gctcgcggcg aagccggtga tggaaggcaa
     3661 gagcgtcctg ttcaagaagt tcgccggcat cgactgcttc gacatcgaga tcgacgagcg
     3721 cgacccgcac aagctgatcg acatcgtcgc cgcgctcgag ccgactttcg gcgcgatcaa
     3781 cctggaggac atcaaggcgc cggagtgctt catcgtcgag cgcgcgctgc gccagcgcat
     3841 gaagatcccg gtgttccacg acgaccagca cggcaccgcg atcaccgtgg gggcggcgat
     3901 cttcaacggt ctcaagcttc tcaggaaaga cattgccagc gtcaagctgg tcgcttccgg
     3961 cgcgggcgcg gccgcgctcg cgtgcctcga gctcctggtc aagatgggat tgcggcggga
     4021 gaacatcgtc gtcaccgaca tcaagggcgt ggtgtacaag gggcggcgcg aagagatgga
     4081 tcccgacaag gaacgcttcg cggtcgagac gcgcgcgcgc acgctcgacg aggtgatcgc
     4141 cggcgcggac gtgttcctcg ggctgtcggc gggcggggtg ctcaagggcg cgatggtgaa
     4201 gaagatggcc gagcgcccgc tgatcctcgc gctggcgaat ccggaccccg agatctcccc
     4261 ggatgaggcc aaggccgcgc ggcccgattg cgtgatcgcc accgggcgct cggactttcc
     4321 caaccaggtg aacaacgtgc tgtgcttccc cttcatcttc cgcggcgcgc tcgacgtcgg
     4381 cgccaccacc atcaacaccg agatggagct cgccgcggcc aaggcgatcg cggagctcgc
     4441 gcaggccgag acctcggagc aggtggcgct cgcctacggc atcgacacga tcagcttcgg
     4501 ccccgagtac atcatcccga agccgttcga cccgcgcctg atcgcgcgcg tcgcgccggc
     4561 cgtagcgaag gcggcgatgg actcgggcgt ggcgacgcgg ccgcttgcga gcctggacgc
     4621 ctaccgggag tcgctcagcc ggttcgtcta ccactcgggg gtgatcatgc agccgctctt
     4681 caccgcggcg aaggctgcgc cgcggcgggt ggtctacgcc gagggcgagg acgagcgcgt
     4741 gctgcgcgcg gcgcaggtgg tggtcgacga gggccttgcc cggccgacgc tcatcggccg
     4801 tccgcaggtc atcgagcagc gcctcgagcg cctcggcctg cgcgtgcgac cgggcaagga
     4861 gttcgacctg gtcaatcccg aatccgaccc ccggtatcgc gactactgga cgacctatca
     4921 ccagctctcc gagcgcaagg ggatcacccc ggagtacgcg cgggtcgaga tgcgccgcca
     4981 caacacgctg atcggcgcca tgctcatgca caagggcgag gccgacggca tgctgtgcgg
     5041 caccttcggc atgcacgcgg agcacctgcg ctacatcgac caggtgatcg ggctgcgcgc
     5101 cggcgtgcgc aactacgcgg cgatgaacgc cctgatgctc gcggcgcgca acgtcttcat
     5161 ctgcgacacc cacgtgaccg aggaccccgg cgccgagcac ctcgccgaaa tgacgctgct
     5221 cgcggccgac gaagtgcggc gcttcggcat cacgccgaag gtcgcgctcc tgtcggcgtc
     5281 gaacttcggc tcggcgcaga ccgcctcctc gcgcaagatg cagcgcgcgc tcgagctgat
     5341 ccgcaacctc gcccccgagc tcgagatcga cggcgagatg cacggcgacg ccgcgctttc
     5401 cgaggagatc cgtctgcgcg tgttcccgcg cacgcgcctg cgcggcgagg cgaacctgct
     5461 ggtgatgccg acgctcgacg ccgccaacat ctcgttcaac ctgctcaaga ccgccgccgg
     5521 cggcggcgtc accatcgggc cgatgctgct cggcgcggcg cggcccgtgc acatcctgac
     5581 cccgtcggcg acggtgcgcc gcctggtcaa catgacggcg ctggtggtgg cggacgtcaa
     5641 cgcgccccgc gctcaggagc aaaacctctt ctgatggaac ctgcgagcaa cgtcgcccct
     5701 ttcccggccg gccgcacgcg cgcccgcgcc gggctggtgc tcaccggcgg cggcgcgcgc
     5761 gcggcctacc aggtcggggt ggtgaaggcg gtgcgcgaca tcctcggcaa cccgacgaag
     5821 aacccgttcc cgatcctgtg cggcacttcg gccggcgcga tcaacgtggc gacgctggcg
     5881 gtgttcgccg acaacttctc gcgcggggtc gccaacctgc tcgaagtgtg ggagcacatg
     5941 cgctgcgagc acatttaccg caccgacgcc tggaacatcc tgcgcgccgg cgcgcgctgg
     6001 ctctcggcga tgatgctgct ctcgcgccac aacccggtct cgctgctcga caacgcgccg
     6061 ctgcgcgaga tgctcgacaa gaacctgaac ttcgagcgca tccagggcca catcgactcg
     6121 ggcgcgctgt acgcggtgtg cgtcactgcc tcgggctaca cctcggggca gagcgtgtcc
     6181 ttcttccagg gcggcagcgg catcgagggc tgggagcgca accagcgcat cggcgccgca
     6241 gtcagcatca agctcgacta cctgcttgcc tccgccgcgc tgccgttcat ctttccggcg
     6301 gtcaaggtgc atcgcgaata tttcggcgac ggctcgatgc gccagatcgc gccggtcagc
     6361 ccggcgctgc acctcggcgc agatcgcgtc ctgattgtcg ggaccggccg ccagcacacg
     6421 gacgacgcgc gcgcgcgctc caacacctat ccctcgctcg cgcagatcgc cggtcatgcg
     6481 ctgaacagca tttttctgga cagcctgatg gtcgacatcg agcggctgga gcgcatcaac
     6541 cgcacggtca agctgatccc gcccgaaagg cttgccgagt cgaacgtgcc gctgcgcgcg
     6601 gtgaaagtgc tcttcatcac cccgtcgcag ccgatcgagc gcgtcgcctc gcgtttcatc
     6661 cacgatctgc cgcgcaccgt gcgcttcgtg ctgcggccca ccggcgccct caaccgctcg
     6721 ggctcgaacc tggcgagcta cctgctcttc gaggaatcgt tctgccgcgc gctgatcgat
     6781 ctcggctatc aggacactat ggcgcgcgag gccgaggtca aggatttctt cggaccgccg
     6841 gaagagaggg cgtatggctg acgacaaccc gcctcaggac cccttcgaga tgttccgccg
     6901 cctgtgggga ccgctcggcg tgccgatccc cgggatggcg atgccgaccc tggacccgca
     6961 ggaggtggag aagcgcatcg ccgacctgcg ctcggtcgag ggctggctga cgatgaacct
     7021 caatatggtg aaactttcga tccagggcct tgagatgcag aaggcgacgc tgcaggctat
     7081 gcagcccaga aaagacaagt aaaacccctc aggagagagc aatggcgaag gcgatccgtt
     7141 atcacaagca gggcggcccg gaagtgctgc agctcgacga catcccggtc ggcgagccgg
     7201 gccagggaca ggtgcgtatc cggcacacgg cgatcggcgt gaatttcgtc gacacctacc
     7261 agcgctcggg cctctatccg atgcagctgc cggcggtggc tggcaatgaa gccgccggcg
     7321 tggtcgatgc cgtcggacca ggggtgaagg acctgaagaa gggcgaccac gtcgcctaca
     7381 ccggcctgcc gggctcgtac tgcgacgagc gcatcgtgcc cgccgaccgc atggtgaaga
     7441 tccccaaggg catcagcgac gagcaggcgg cgtcgatgct gctgaagggc atgaccgtgc
     7501 actacctcat ccactccacc tacaaggtga agaagggcga catggtgctg tggcacgccg
     7561 cagccggcgg cgtcggcctg atcgccggcc agtggctgaa gaagctcggc gccgtcacca
     7621 tcggcacggt cagctcagac gagaaagcca agctcgcgaa ggcgcacggc tacaagcacg
     7681 tgatcaacta cagcaccgaa aatttcgtcg agaaggtgaa ggagatcacg aaagggaaaa
     7741 agcttcctgt cgtgtacgac gcggtaggca agaccacc
//
LOCUS       DATBSZ010000058        35402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_621811, whole genome shotgun sequence.
ACCESSION   DATBSZ010000058 DATBSZ010000000
VERSION     DATBSZ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 35402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35402
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621811"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VIG70_03670"
     CDS             <1..559
                     /locus_tag="VIG70_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8249679.1"
                     /translation="VSTESGQVDFAADIVKLKAANADVIFVYTNEEECARVLREARKQ
                     GVKAPIIGETNVISQKVIDLAGDAATGVKGHVGLTADAPIKAVLEFRDKFQKRFKYLP
                     DHNGIKGYTAVYAVKHATEKLGKFDSKQLARSLHGMTITPQQEPGILIETTWDARGDI
                     DRISFLVEVADGKQKVVEILPKLGK"
     gene            594..1439
                     /locus_tag="VIG70_03675"
     CDS             594..1439
                     /locus_tag="VIG70_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8249680.1"
                     /translation="MATGGIYALVAVGFVLLWQTSQTINFAQGEFVMLPAFFVLAAMK
                     FGGLSFPAALLVGFALAALVLGLLFKRLLVDPILRHGVLPLVISTLGLAILMKEAVKE
                     FYAAEAQSFPGMWPETNVSIGGAVISAQSLVIIGVALAAVALLQYFLGATRTGRQMQA
                     TAQNPMLARVLGIPVERMILYTFLVNAALVALASFLVSPIYLAKFTNGELLGLAAFVA
                     AIVGGFNQVRGAIVGGLLLGMVDNFVAAYWSAQYRAAIPLFLLIAVILWRPQGLLGRA
                     EERTV"
     gene            1436..2359
                     /locus_tag="VIG70_03680"
     CDS             1436..2359
                     /locus_tag="VIG70_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8249681.1"
                     /translation="MKKLFLLLLAVVLIAAPFFLKPYGIYLLTLWVVMTIAAIGLNLT
                     LGYAGQISLAQGAFVGIGAYAAALLTTQGWPLWSAFLLAILLSLAIGWLLGYPALRVQ
                     HHYLAFVTLSFATLVYLMLRNEEWLTKGVYGVSNIPRPTLLGWSTKGPVAFYFFCLGV
                     LALVSAATWWLVRSPWGRAFTALRENAMRADSLGVDTRRYTLMAFAFGSAIGGIAGVL
                     YAPMVQFIEPTAFNLNLSLNLLLMVMVGGSGFFLGPFLGAAVSVLLPEWMRFGSEGLY
                     LIFYAIAVMVLMAFCPSGLLGLAERARRKFG"
     gene            2356..3111
                     /locus_tag="VIG70_03685"
     CDS             2356..3111
                     /locus_tag="VIG70_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249682.1"
                     /translation="MNAPPRAVLEVRSLAKAFGGIHAVNGVSFDVREGEILGLIGPNG
                     SGKSTLFNCILGQLPPSAGEVRIDGRAITGMRPCQLNRLGVGRTFQLLQVFPQLTVRE
                     NLILAGQEHQASMWSRLVGRRDAGLGPAADRMIEFFRLAQLAEEKAAALSYGQQKLLD
                     VAMAFMAGPRLVLLDEPAGGVNPTMLAGLRERLREINAKKRATFVVIEHNMEFVMSLC
                     TRVLVLAEGRIIAEGTPEDIRGNPQVIEAYLGG"
     gene            3116..3835
                     /locus_tag="VIG70_03690"
     CDS             3116..3835
                     /locus_tag="VIG70_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249683.1"
                     /translation="MILENVVAGYGGLTVLNGTSLEAARGAITTIIGPNGAGKSTIFK
                     TVFGLLPARSGRVVFEGREVTNLGPRKLLELGISYVPQGRNIFPELSVRHNLELGATA
                     ARDGIEVAARIEAVMDRFPMLRERAAAQASTLSGGQQKLLEIARSLLLDPRLMLIDEP
                     SIGLSPRLVQETFAILQDLRAKGMSILMVEQNAKRALEASDYGVVIELGRTRMAGEAR
                     AILADPKVGQLFLGGGLAATG"
     gene            complement(3825..4796)
                     /locus_tag="VIG70_03695"
     CDS             complement(3825..4796)
                     /locus_tag="VIG70_03695"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8249684.1"
                     /translation="MNRRDLLRWLAGSLLLGSGTSLAQRGSPPRVGWLSYLPEPDPAL
                     ALLREGLSKLGHVEGKSYVMSARYANNDFTRLPALVAELVADRIDVLVSRGPSVDFTK
                     PVRDRVPVVFVYSGDPVAAGFADNLGKPGRNMTGMTFMAMELCAKRVEVLKDLLPGAK
                     RIALLSNPEHAGELLEYRVTEETARRLGAETTRYLVHKPQELAPAFAAIRAAKFDATL
                     VFPDSLTLNRRKEIAEMAAETRMPCMYGWTEFVDAGGLASYGPTITEGFRALARFTDK
                     ILKGGNASEIPIEQAQSISLSLNMTAAKALGMKIPPALLARVDRFIQ"
     gene            complement(4806..5366)
                     /locus_tag="VIG70_03700"
     CDS             complement(4806..5366)
                     /locus_tag="VIG70_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249685.1"
                     /translation="MAFARLSSLALLFVACVAVAQAPTRVRGTISSLKEDVLTVDGKT
                     QVRLGEKTEIVFTQPVALADIKPGDFLGVTSVKHGDGKLIAYEVRRFPKPLNPGHRPF
                     DGRDDQTMTNASVGAVVESVEGAARGREFTMTYEGGMQKIVVPESASVSTLVPGNRSQ
                     LAVGAPVNLTHDPSGVAIRIQVGPKR"
     gene            complement(5344..5916)
                     /locus_tag="VIG70_03705"
     CDS             complement(5344..5916)
                     /locus_tag="VIG70_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249686.1"
                     /translation="MKHLALLALVFVSSLAIAQAPTRVRGTITALDGDVLSVKSRDGK
                     DLKVHLASDVGVSTAKSVKLDELKDKYVGVTAITKDGKMTAVEVHAIPPQAKPGHFPW
                     DLQPDSTMTNANLEGIAQASGGNEITMNYQGGSQKILVPPGTPIVVFVPGSRADLKPG
                     ESIFTIAGQQGDKLLAQRISVSKDGVRPPQ"
     gene            complement(5927..6880)
                     /locus_tag="VIG70_03710"
     CDS             complement(5927..6880)
                     /locus_tag="VIG70_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249687.1"
                     /translation="MKRLAFLLALGIAWPAAAQFPERPLRLVVPQAAGSATDNIARIV
                     APALAKELGQAVVVDNRPGGALTIGIDAVAKAPADGYTLGQGPVGALAITRHMVAKLP
                     YDIERDIQPVALVTTGYMLLAASPKAPFQSVAELIDYAKKNPGKLSNASSSNGSPGHV
                     SGELFKYMTGTDIVHIPYKGGAPAIADLISGQVQLMFESTNSIASHVRAGRVRALAVT
                     GERRAISLPEVPTLIEAGVAGYEVNAWSGIITTAGVPRAVLDKLNAAVNRAIVHPETK
                     ERLAQLGSEGGGGTPEEFAALIRRDSAKWADVVKRSGAKID"
     gene            complement(6877..7308)
                     /locus_tag="VIG70_03715"
     CDS             complement(6877..7308)
                     /locus_tag="VIG70_03715"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY8249688.1"
                     /translation="MSKLTVKLVLAIAAVVLAGCSSMPAWMPGSGAIKVSLTGAEEVP
                     PVSVPGSGSGSFRVAEDGTITGSVTTKDVQGTMAHIHRGAKGVNGPVIVPLDKNGDTY
                     TAPAGRKLTPEQINDLKAGNLYVNVHTNRNKGGEVRGQLAP"
     gene            7357..8550
                     /locus_tag="VIG70_03720"
     CDS             7357..8550
                     /locus_tag="VIG70_03720"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8249689.1"
                     /translation="MPRIAASTALARLRILDFSRVRAGPTCVRQFADFGADVIKIESP
                     PGVDPNEAMGGPRHGPDMQNLHRNKRSMTLNLKLPESRGVLERLAKTADVVVENYRPD
                     VKFRLGIDYASLEKINPRIILASISGFGQDGPYAKRPGFDQIAQGMSGLMSVTGLPGQ
                     GPVRTGAAIADLTAGLYAAIGVMIALLERETSGKGQWVQSSLLQAGISLLDFQAARYL
                     MEGDVPPQVGNDHPTSMPTSAYKTKDGHINVAASGEGMWAGLCKAIGREDMLASPDFK
                     GGKKRAENRAKLNSILNESLSRKTGAEWIEILNKAGIPCGPIYKVDEVFADPQVKHLG
                     VATAVEHPKLGRKEILANAVALSRTPAKVVAPTPEIGAHTDEILKELNYNAAEIAKLR
                     EGGVV"
     gene            8547..9497
                     /locus_tag="VIG70_03725"
     CDS             8547..9497
                     /locus_tag="VIG70_03725"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249690.1"
                     /translation="MKKLIAFAGILFSGICFAQYPNKAVTIVVGFEPGGGTDTTARIV
                     QGPLGEQLGQQVVVENRAGAGGNIAVDYVAKQAPDGYTLVLANVGALAVNPHILKTPY
                     DPLKDLVPISMAAEFANVLVVQPSLGVNTVADFVKIAKAKPATVTYASSGIGGAGHLS
                     GELLRRMADIDIVHVPYKGGGPAMQGFLGGQVASFFATPISSISQIRAGKAKAVATTG
                     SKRAALMPDVPTVAESGYPGYEALNWYGFLGPKGLPKEIVERLNREIVKALAHPQVVS
                     ALHKTGTEPKSSSPEEFAAFIKREYDTWGKVVKEAGIKAQ"
     gene            complement(9498..10433)
                     /locus_tag="VIG70_03730"
     CDS             complement(9498..10433)
                     /locus_tag="VIG70_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY8249691.1"
                     /translation="MRAYFLKSPEMQLELRDAPAPQPKPGQHLVRVRAAGLNRGELLR
                     HGLTKPGPAKIGGTEGAGEVEGSGARVMGRLPGSFAEYALMDAEDAIPVPQNLSWEEA
                     AAIPITFLVVYDMLVQQGSLKKDEWLLVTGVSAGVGVAALLAAKALGAKVIGTSGSDE
                     KLQKLKSLGLDVALRTRNPDFSAKVLEATQGKGVNLVVNNVGGSVFAECVKSLGFEGR
                     LATVGYVDGVMKSEIDIEALHSKRLKLFGVSNKMRNAAQRGVTVAGFNRDFLPLFASG
                     KLKPVIDRVYGFQELPEAKARMEADAHVGKLVVRI"
     gene            10545..10901
                     /locus_tag="VIG70_03735"
     CDS             10545..10901
                     /locus_tag="VIG70_03735"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEY8249692.1"
                     /translation="MMQRLGKWFVDFWIVLAFGLAAALLMPPVHAEMIGTESVQAQDE
                     RARVKALLERPELAKGLERMGIAPRDAVGRVDAMSDEEVLQLAGRLDAALAGGQMSNE
                     TLLLIIIVILLLIIIL"
     gene            complement(11003..12385)
                     /locus_tag="VIG70_03740"
     CDS             complement(11003..12385)
                     /locus_tag="VIG70_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEY8249693.1"
                     /translation="MSDETAALEDYAGRIESLAHRLGLDYYPVDFEMAPQSLMTEIAV
                     YGLPIRMPHWSFGVRYIHQLVRQSMGNSKIFEVMFPGDPCRAFLMDTNSLAENALVTA
                     HVVGHADFSKNNQLFARFHDMAGGNIIEKAAVHAQRIQQAIDTAGLERVEAVLDAALA
                     LESHIDVGGELHRSRYPEFIADKPKAQASHFQKRFQELPGASRPEALPLGLTLGPQRT
                     RVPPHPEYDLLWFIANYAPELEQWERDIFLAVRAESLYFYPVFACQIMNEGWASYWHA
                     RLLREADFLPQTTYLEAIKAHSDVVRPYASGEQTALAINPYHLGFTLWETIVERHGLE
                     RARQVMREEDDFGFIRNWLDRELAEKLDLFVFEARPNDEIRVANRDLNAVREAILAPK
                     FNFGAPRVAAVEIKPDGGLVLAHEHSSDGRGLDAGRARKVLEYARRAWRRPVRLHTIN
                     DRSEAIELAA"
     gene            complement(12378..13529)
                     /locus_tag="VIG70_03745"
     CDS             complement(12378..13529)
                     /locus_tag="VIG70_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEY8249694.1"
                     /translation="MTLRIAAETPWYDLFSRGARDWLRHNQKVRSAVKESLPELLAGG
                     DLITRPGNRTVAVPVKMLEHSRFRLLDADAQQGAGQGKGQPGDVLQPGREDGDSQGRA
                     GGTGNGEIRFVVELKIDEIVDWLWEELKLPELKPKRSAALDEPDYVREGWDKRGARSR
                     LDRRRTLKESIKRRSIQTETVAIVNDDLRFRQLAKRATPATNAAVIFALDVSGSMDEA
                     QRRLAKQFFFFALQGIRRQYTKVETMFLAHAAEAWEFDESQFFQASSSGGTVTSSVAS
                     LALDTVQKRFDPSRYNTYFFYASDGENAAEDRDAARSALGELCKLVNYAGYCETGGVA
                     TFRPRETQLAELFGELKNGGAPVGVSHLGSQEDIWRAIREFFSQQAANE"
     gene            complement(13526..15451)
                     /locus_tag="VIG70_03750"
     CDS             complement(13526..15451)
                     /locus_tag="VIG70_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEY8249695.1"
                     /translation="MPVRAISQNDETREAGRSNLIRSLSDFTKQHRAANWTGSFAAFL
                     ESVLPADSRGIARSSHQYMWDMMRSKDTLFQDELYGIDDTLERVMEYFKAASAGSEVG
                     RRLLLLLGPPSGGKSSMVILLKRGLEAYSHTEAGALYGIDGCPVHESPLHLVPHTMRA
                     QFRQTYGVDIAGELCPYCTSRLQHEFDGDFMQMPVQRIFISEAGRVGVGTYAPHDPTT
                     ADIADLVGSVDLSKVSQFGDEGDPRAWSWSGAVFAASRGALEMIEILKVKREFLYLLL
                     TLTQEKNVKVSRFPLIHLDETILAHTNLAEFRKFLQEPENEALLDRMVIIQVPYTLSY
                     KEEAKIYRKLTSSTQAFREVHLDPHALHVAAAFSVLTRLAESEGESDGTKKARLYAGE
                     DVDGVSGNEVNKIRARNPEEGLAGVSPRFVINALSSAIIQSAKSSLTTMDVLIALKDA
                     IEADVRIDGKKKRKWVDFLVTVRKEFYNRWVKEDVHKALFVSFEQEAQQLLDKYLDEV
                     EATLDNRTLKDPITTEERKPDERFLRNVEEKIRISESGKLSFRQEVVRKAMGAYKRGE
                     KFTLDSHAPLHDAVEQYLFEQRRDVLRLVSSSARPDDETRQKISVVEKRLVDEYGYDS
                     HSAREALNYVTTLLAQE"
     gene            15713..16063
                     /locus_tag="VIG70_03755"
     CDS             15713..16063
                     /locus_tag="VIG70_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249696.1"
                     /translation="MLKEILGVADDPPAKRRWFHDEYFDLFVWQAQGEVTLFQLCYGE
                     DATERALVWDKERGFFHDGPASRDVVALFEDAATALPPDLAAEIRQKVHEFAGRQLTI
                     VSRRKKFRRAAWQR"
     gene            16118..16504
                     /locus_tag="VIG70_03760"
     CDS             16118..16504
                     /locus_tag="VIG70_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY8249697.1"
                     /translation="MKPELMWLLWAVALTFAQMLVAVGGATLQVGLPMLAGNREGLAP
                     CTGWAGRAQRAHHNMLENLVLLAALVLMAVVSGKTNAMTLLGAQLFFWARLAYAFVYL
                     AGIPWLRTAVWLVSVIGLLLIFVQLL"
     gene            complement(16482..17555)
                     /locus_tag="VIG70_03765"
     CDS             complement(16482..17555)
                     /locus_tag="VIG70_03765"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY8249698.1"
                     /translation="MKPIHLMLLLAAFSAQATEPDNLRRLRVPPPPWPQGDERGMANQ
                     IGPATFQRCAWHMQQPGARSYELSYLRSNTMPLSPFAGPYAVKPKASAGIPGTAHAFN
                     SEQMNENAEPAQQGTQMDALGHFGAFKQPWDGKTEFPAGEAVYYGGFTQKDVKPTPDS
                     PLLKLGIDKAPPIITSAVLLDAKAYKKAGMKDGELVSAKDLEGMLKAQGLAGRGVLPG
                     DVVYVRTGWGDHWRDPDTEKFYYTKAPGLSFDAAQWLAEKRIVAIALDTPFVDTVAEG
                     MLMGKAGPAPGTPPGLPFAVHHHMLSQAGIHLVENAKLDELAADRVWNFCTLILPLRD
                     QGAAGSAVRPVAIGVPPIRAGRK"
     gene            complement(17552..18373)
                     /locus_tag="VIG70_03770"
     CDS             complement(17552..18373)
                     /locus_tag="VIG70_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY8249699.1"
                     /translation="MPKFFDRIGVDINQKLPLERAIAWAIEHKVPVIDLCLDQTPDLL
                     EKSSPRLANARKHLEANDIKLGLHTLSAVNIAETSPFVTSAADAYLAAYLDAAKKLGA
                     GWVVVHGGYHFGGDKRARMDAAVARIARAAKAAEKKKVKLLLENLNPEPEQAEVHYLC
                     NDLEECRYYFERLDSPALGWSFTVNHAHIRPNRIDAFMAGLDMSRCGEVRLADCRGTH
                     EEHLRPGEGTIDFGAMFAAIEASGYRGHYMQAFGSLDDMLKGRSTLAQLAEEGLK"
     gene            complement(18357..19835)
                     /locus_tag="VIG70_03775"
     CDS             complement(18357..19835)
                     /locus_tag="VIG70_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY8249700.1"
                     /translation="MELLGHLAQGFLAAATPANLLMCLLGVVLGQIIGVLPGIGPSAA
                     IALLLPLTYGASPTGAIIMFAGLYYGAQYGGTLTSVLINVPGESTSVMTTLDGYQMAL
                     QGRGGVALGIAAIGSFIAGCLGTVGLMLLAPPLAKVALAFGPPEYFALVLLGLMALAA
                     VGGSVLKGLATGVAGLLLGTIGIDPQVGAPRFDFGQLWLLDGVDFIILAVALFGVGEV
                     LASCRMGSATPILNVGRVLPNREEWRQARMAILRGSGIGFLIGILPAAGATIASFVGY
                     IAEKKLAKDPSRFGKGAIEGVAGPESANNAASAGAMVPMFALGVPGSNTTAVMLAALI
                     MFGLRPGPDMFTTNAALVWAVIASMFIGNLILLVMNLPLAGLFARLLQIRYSWIYPPI
                     LAICVTGVVSRANNVEDAWLMLGFGVLGWLMKRYDWSAAPMILGLVLGPIFENSLRQS
                     LTLSHGSGLIFVTRPIAAVLVAFALIAVTAPLWSRFFRAKIL"
     gene            complement(19846..20298)
                     /locus_tag="VIG70_03780"
     CDS             complement(19846..20298)
                     /locus_tag="VIG70_03780"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY8249701.1"
                     /translation="MLNRPAPGEVLLALLFAVLGILWIVAAARMPLWAGFVPQSGFMP
                     LWYGIILVALAAAILANLFLRRDPGQKEEPIGKPLVVLAALAASIAGLDLVGFAPSIF
                     LLLLVLFAAVERLPMVRSLVVATTATAVLFLIFRTWLRVPLPVGPLGI"
     gene            complement(20312..21253)
                     /locus_tag="VIG70_03785"
     CDS             complement(20312..21253)
                     /locus_tag="VIG70_03785"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249702.1"
                     /translation="MRFFAKLLLLAALPVAASAQGWKPTKDVEFVIPFAVGGGADLMA
                     RVIHKIITEDKLVPVPVALVNKPGGGGAAGIGYMSASRKADPNTLVLINGSTQITPII
                     TPTAKTLTEVHPVMNVMLDDFVFFVRGDAPWKTAAEFVKDAKSKPAKTYNFSTGGTTD
                     VMAVTVLAKSTGTELNMISFNSGGEALTALLGGHVHASLGNPLEFMGHLQKGTVRAIG
                     VFRDTRFAMLPNVPTMKEQGINAPTFQMWRGVGVPKGVPADAAKYWEGVMAKVVASPQ
                     FKAYLKDNAASEATIAGAEFEKFLATQERLYRDLLGK"
     gene            21304..22191
                     /locus_tag="VIG70_03790"
     CDS             21304..22191
                     /locus_tag="VIG70_03790"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY8249703.1"
                     /translation="MGMLVEGVWQDIPRDTKATGGAFVRAESAFRDWVSEPEAGRYRL
                     YVAKSCPWAHRTLVARALKGLEKAVPVSYADPYMGENGWQFPEGYLHELYAKAKPGYT
                     GRVTVPVLWDEKQGRIVNNESSEIIRMLNREFDALARRKLADLYPEKRRREIDWLNER
                     IYRTVNNGVYRAGFATAQDKYEEAVSGLFRTLDWLEKRLAKRKWLCGNDFTEADVRLF
                     TTLVRFDAVYYSHFKCNLRRLVDYPNLWRWTRRVYALPGVAKTVDMAQIKSHYYRSMK
                     HINPTQIVPKGPLLDFSLH"
     gene            22233..23513
                     /locus_tag="VIG70_03795"
     CDS             22233..23513
                     /locus_tag="VIG70_03795"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY8249704.1"
                     /translation="MLAPGEAGIGFTWRFEPSPYRGSDRDSDLMMQFRYDSRYFYLQS
                     DRIGLKLESERYRYELFLRRRLEGFASDHVPESMTGMAKRSSGDDLGVAARYRLGAGV
                     VYGEAMQNVSEESDGAELRLGYRYEGWWSGKLRWRPYATLAWRDSKLNNYYYGVRPEE
                     ATAERPEYKAGSGLNLELGVLAAYRFAERWQLLAGAGMTRWSSSVRGSPVVEDGLQPY
                     AMLGLMYGFKPDQSSEWDRRPLIMRVGYGQSSDCDLTPIVTLQCTSVNTQDDTSIVSL
                     EVGQILVRGLNGWPLDLAGFVGFLKHEERGLQEDSWQINGYFKAYYYLGSWREWLRTR
                     LGLGAGISYASRVPFSEQRDQALRGRDTSKLLLYLDPTVDINVGDVLRARALRETYVG
                     LGVSHRSGVFGASRLFNSVNGGSNYIYGYVETSF"
     gene            23494..25128
                     /locus_tag="VIG70_03800"
     CDS             23494..25128
                     /locus_tag="VIG70_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY8249705.1"
                     /translation="MSRRASSLLLVLVALAWLGSLALRPLYKPDEGRYGEIPREMAAS
                     GDWLTPRLNGFKYFEKPPLQYWATAAAFKVFGVSDWAARLWTGLAALAGVLMVFAAGN
                     RLYAPPAGAFAAAILASSPLYVLLGQFNTLDMSVTLFLSAAVFAFALAQRAASEPAVR
                     RRWMLAFWAACAAAVLSKGLIGIVLPLGAVALYVPARRDWALLRRLELVRGGLLFLLI
                     AAPWFVAVSRANSEFAHFFFVQEHWLRFTTRMHHREHPAWFFLPVLVAGMWPWLLAVI
                     AGWVAALRPAPGKEFSPALFLGLWALVVFAFFSGSGSKLPPYILPLVPALALLAGRLL
                     ARGPRSGLLMAQSLLVAAAAMATGGGAWRLTGAGDGALYGLGDDYAPWLAAAATIGLA
                     GAVGAAALAALGRAAGAVASLAAGALAALLVASGGHAVFAPAYSVAPLVASLAQAPSR
                     DAAIFAVQAYDHTMPWALRRTVTMVGYKDELEQAVAWEPDRFIPDLAAFARAWNAERE
                     AYAFFAARDFERLRRELDLPMEIAARGPRYVIVRKP"
     gene            25125..25463
                     /locus_tag="VIG70_03805"
     CDS             25125..25463
                     /locus_tag="VIG70_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEY8249706.1"
                     /translation="MTLFSFWFILAGVLLNAAAQLLLKAGTNAMPLGLRLALEPHILA
                     GLACYVVSVVVWVVALSRVPVSIAYPMLSIGYVVNAIAAWALLGEAVTPMRMAGIAVI
                     VAGVFIVARS"
     gene            25460..26584
                     /locus_tag="VIG70_03810"
     CDS             25460..26584
                     /locus_tag="VIG70_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEY8249707.1"
                     /translation="MSAFLPFARPEIDEQTIAAVAEVLRSGWITSGPQVLRFEEALSA
                     HCGGRPVRTFTSATGALEVALALAGVGPGDEVIVPAMTFVASANVVLRAGARPVLVDV
                     DLDSRNTDAARIEAAITPRTKAVMPVHFAGLPVELAPVCALAKRRGLRVIEDAAHAMG
                     ASHAGRAIGAQGDLVAFSFHPNKNITTIEGGALSFADPAEVERAERLRFHGIRKLPGG
                     EMEVEEPGGKFNLADVAACIGLAQLARLAQFNARRSELARRYLELLQGMRHVRLPAAD
                     RPGHSWHLFAPLVDFGALGITRRALIERMHARGIGAGVHYQAMHLFAAYRRLGYGPGD
                     FPNAETIGARTVTLPLFTQMQNADVERVCAALQAAILGRE"
     gene            26589..27587
                     /locus_tag="VIG70_03815"
     CDS             26589..27587
                     /locus_tag="VIG70_03815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8249708.1"
                     /translation="MAAAPKVSIVVPIYNEEAGLPALLARLYPALDALGQTYELILVD
                     DGSRDHSIRVLRQQFDRRPDVTRVVILAANVGQHMAIMAGFQHARGEVVVTLDADLQN
                     PPEEIHKLLAKMEEGFDYVGSYRSERRDNLFRRAASRAMNRLREDLTRIRMADQGCML
                     RAYARNVVDAINQSPEVNTFVPALAFIYARNPTEIEVRHDERAAGASQYSLSRLIKLN
                     FDLVTGFSVKPLQIFSGIGMIVAVGAVLLYATVLAYQLYTAGLAGALEHVWDRDVLEF
                     FLMGLAVFGIGLTGEYIGRIYEQVRGRPRYMVQAVLEQQPGAQQWQPRQSSSRITT"
     gene            27548..28396
                     /locus_tag="VIG70_03820"
     CDS             27548..28396
                     /locus_tag="VIG70_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HEY8249709.1"
                     /translation="MAAQAVVFAYHDVGVRCLRVLLEAGIAVPLVVTHRDDPAERVWF
                     ASVAELARKHGIETLQDAETAQPLERLRTLGPDFIFSFYYRRMLAPGLLALARRGAFN
                     MHGSLLPKYRGRAPVNWAVLMGETETGATLHEMVAKPDAGRIVDQERVPIGPDDLAVE
                     VMGRVAVAAEVVLRRSLPALLSGTARLRPQDLSRGSYFGGRKPEDGRIDWSRSAREIH
                     NLVRAVAPPYPGAFTHGMKINRTRIEPQRQAPRGALGPYRENGEWFAACGDGRVLRLL
                     EIEPLQ"
     gene            28393..29436
                     /locus_tag="VIG70_03825"
     CDS             28393..29436
                     /locus_tag="VIG70_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HEY8249710.1"
                     /translation="MKRLLILGVNGFIGHHLSKRVLASTDWEIYGMDMQSDRVEDLLG
                     ERRFHFFEGDITINKEWIEYHIRKCDTVLPLVAIATPSVYVKDPLRVFELDFEANLPI
                     VRACVHHRKRLLFPSTSEVYGMSPDREFDPEDSPLVYGPISKQRWIYACAKQLMDRVI
                     WAYGDKVGLDFTLFRPFNWIGAGLDSLATAKEGSSRVVTQFLGHISRGEPIQLVDGGR
                     QKRAFTYIDDGIDALMRIIDNPRGVASGKIYNIGNPRNNLSVRQLAEMMVELALEIPE
                     YQKAARRVRLRRTTAERYYGRGYQDMQNRVPKIDNTKRDLRWHPRVGMRDALRRIFEA
                     YRTEIAEARRLVG"
     gene            29433..30317
                     /locus_tag="VIG70_03830"
     CDS             29433..30317
                     /locus_tag="VIG70_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY8249711.1"
                     /translation="MKLALKVDVDTWRGTRDGVPRLVELLKRHGAGATFLFSLGPDHT
                     GRALRRVFRPGFFSKVRRTSVLRHYGLGTLLYGTLLPGPDIGRRGASTLRAARDAGFE
                     VGIHCWDHVRWQDHVALADSDWTERQMRRARERFTAIFREIPRTWGAAGWQTNRHAAW
                     VEEREFDYASDTRGRGPFRPLWDGVAIGCPQLPTTLPTLDELIGVDGIDEGNVAAHLL
                     DLTAQKSPHVFTAHAELEGGALLGAFEALLRGWKAQGYELVALRSLFESLDLAQLPRH
                     DVVYGEIPGRTGKVALQA"
     gene            30314..30988
                     /locus_tag="VIG70_03835"
     CDS             30314..30988
                     /locus_tag="VIG70_03835"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY8249712.1"
                     /translation="MILRGVALFLLLMASAHAELPPRLQITYDVLRNGSAVAEIVGRL
                     ESDGNTYRLTESWKGRSFYALLGRAMRTSEGSLTPALVRPREFTDERSGRDTARAWFD
                     WGANAMTLRYKGKSRTEPIPPNAQDRLSFILALSAAPADAKHGDYHLVDGRGVSHHIY
                     EFAGRERVQTPAGEFDARKIVRGAKDDDQVQIWLAKPLGGLPVRMLVVEKGGTRWDQV
                     ATRVER"
     gene            31082..31807
                     /locus_tag="VIG70_03840"
     CDS             31082..31807
                     /locus_tag="VIG70_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8249713.1"
                     /translation="MNAPGKVAVGDGIAAGNARWTFAGDVARTFSAHVSKSVPLYDEG
                     HYLICALSDFFVQDSSICYELGSSTGALLSKLATRHSATKARFVGIEIVPEMIAQARA
                     ESGSLPNLEFAQEDINLYPFEKADLIVSFCTIQFVPPYLRQTLFDRLYQCLNWGGALL
                     LYEKVRAPDARFQDYMTSVYTDFKLARGYSPDEIIAKARSLKRAQEPFSTQGYLDLFK
                     RAGFVDVMSVIKWVCFEGFLAIK"
     gene            complement(31800..32576)
                     /locus_tag="VIG70_03845"
     CDS             complement(31800..32576)
                     /locus_tag="VIG70_03845"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY8249714.1"
                     /translation="MPRFDPRAQRCIGVIKLGNALFRHRNSVGPVVFLVALIFSRPHL
                     PYGSTDLRIAFEIAGLIVALAGESLRILAIGYEYIVRGGRSRRVYADSLVRGGMFALC
                     RNPLYAGNILIAIGLSLVVHAHAFYLIVISAIVLAYHSIVLAEEAYLRGKFGAAYEDY
                     CRRVNRWWPRLASLRRTQPALRFNWQRVLVKEYGTILLVAATLVGLHFWGEYSIAGAG
                     ALPSAAALGCGAIAWSAVYLAVRTLKQRGYISDRPRGLIT"
     gene            32672..33355
                     /locus_tag="VIG70_03850"
     CDS             32672..33355
                     /locus_tag="VIG70_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8249715.1"
                     /translation="MRVLIVEDSPDILANLYGYLEPLGYSLDCARDGFAGLELAAHGA
                     HDAIVLDLMLPGLDGLDLCRRLRRELRRSTPVLILTARDTVEDKVLGLRSGADDYLVK
                     PFSLNELDARLQALVRRSRGLVSATLLRVGTLRFDPATYEATREGRRLELSPMGYRIL
                     GVLMRASPALVTRDTLERELWGDERPSSDSLRTHIHALRQALEKPFAQPVLVTIPGLG
                     YRLIDPDAA"
     gene            33378..34625
                     /locus_tag="VIG70_03855"
     CDS             33378..34625
                     /locus_tag="VIG70_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8249716.1"
                     /translation="MLAFVLLAVVIGGAFAGAAYLIIEATEYQLIELRLAHSAARFFE
                     HDPPQSANPFAGVTAAVGTQIPGELGGLAPGHHELHLPGRDVHVLIGYHAGERYAIIE
                     DTSDFERLEQLAFVGLWVAFLSGVLLALAMARAVVNRVIAPLTSLASAVQRDDLREQP
                     QLLNAADEIGVLARAFDARASQLREVLLRERLFTADVSHELRTPLTVMLGAAELLSAR
                     LAAQAELRAAAERIRRTAAETSVRVSALLQLARSPETIESTLLSLRDLVKQEIERCRP
                     LLDGKPVSLNLESSGNVCVHSAHDLAAIAVGNLLRNACTFTQRGAVRVVLEPTGLVIE
                     DTGPGVPPEVRDRLLARFVRGRAEPATGSGHGLSIVKRVAEHLGWAVRLEDGPQGGSR
                     FTLIFVSEAPRRTRAMSAISDRA"
     gene            34726..35097
                     /locus_tag="VIG70_03860"
     CDS             34726..35097
                     /locus_tag="VIG70_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249717.1"
                     /translation="MSQTATDDALSYCARIRDLILHHGGGWADGDALRKFRLLSDAAR
                     RAADDAEATELMRTAAEYAADLFSGSDHQKWATGRTSGADILRLCILAKLDAFRDRVV
                     HLQEAARGRDPSDRMHYPWDE"
     gene            complement(35079..>35402)
                     /locus_tag="VIG70_03865"
     CDS             complement(35079..>35402)
                     /locus_tag="VIG70_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8249718.1"
                     /translation="LFGAAFVAPYGASKGGIVQLTRALACAWARDNIQVNAVLPGWID
                     TALTQRARKDIPGLQENVLRRTPAGRWGEPQDLSGIAVFLCSKASDFITGAAIPVDGG
                     YSSQG"
BASE COUNT         5557 a        11814 c        12072 g         5959 t
ORIGIN      
        1 cgtgtcgacc gagtcgggcc aggtggattt cgccgccgac atcgtcaagc tcaaggcggc
       61 gaacgcggac gtgatcttcg tctacaccaa cgaggaagag tgcgcgcgcg tgctgcgcga
      121 ggcgcgcaag cagggcgtca aggcgccgat catcggcgag accaacgtca tcagccagaa
      181 agtcatcgac ctggccggcg acgccgcgac cggcgtcaag gggcacgtgg gcctcaccgc
      241 cgacgcgccg atcaaggcgg tgctcgagtt ccgcgacaag ttccagaagc gcttcaagta
      301 cctgcccgat cacaacggca tcaagggcta caccgcggtc tacgcggtga agcacgcgac
      361 cgagaaactg ggcaagttcg acagcaagca gctcgccagg agcctgcacg gcatgaccat
      421 cacgccgcag caggagcccg gcatcctgat cgagaccacc tgggacgcga gaggcgacat
      481 cgaccgcatc agcttcctgg tcgaggtggc ggacggcaag cagaaggtgg tggagatcct
      541 gcccaagctg ggcaagtagc ctggccgact tcctccagct cctggtcgcg gggatcgcga
      601 cgggcggcat ctacgcgctc gtcgcggtcg gcttcgtgct gctgtggcag acctcgcaga
      661 ccatcaattt cgcgcagggc gagttcgtca tgctgcccgc gttcttcgtg ctggcggcga
      721 tgaagttcgg cgggctgtcg ttcccggcgg cattgctggt cggcttcgcg ctcgcggcgc
      781 tggtgctcgg gctgctgttc aagcgcctgc tggtcgaccc gatcctgcgc cacggcgtgc
      841 tgccgctggt gatctccacg cttggcctcg ccatcctgat gaaggaggcg gtcaaggagt
      901 tctacgccgc cgaggcgcag tcgtttcccg ggatgtggcc ggagaccaac gtctcgatcg
      961 gcggcgcggt gatctcggcg cagagcctgg tgatcatcgg cgtggcgctc gccgccgtcg
     1021 ccctgctgca gtatttcctc ggcgcgaccc gcaccggccg gcagatgcag gccaccgcgc
     1081 agaatccgat gctcgcgcgc gtgctcggca ttccggtcga gcgcatgatc ctctatacgt
     1141 tcctggtcaa cgcggcgctg gtggcgctgg cgtcgttcct ggtgagcccg atctacctcg
     1201 ccaagttcac aaatggtgaa ctactggggc tcgctgcgtt tgtcgccgct attgtcggcg
     1261 gtttcaatca ggtgcgcggc gcgatcgtcg gcggcctgct gctcgggatg gtcgacaact
     1321 tcgtcgcggc gtactggtcg gcgcagtacc gcgccgcgat cccgctgttc ctgctgatcg
     1381 cggtgatcct ctggcggccg caggggctgc tcggccgtgc cgaggagcgc acggtgtgaa
     1441 gaagctgttt ctcctgcttc tcgccgtggt gctgatcgcg gcgccgttct tcctgaagcc
     1501 gtacgggatc tacctcctca ccctgtgggt ggtgatgacg atcgccgcga tcggcctcaa
     1561 cctgaccctc ggctacgccg ggcagatctc gctcgcacag ggcgccttcg tgggcatcgg
     1621 ggcctacgcc gccgcgctgc tgacgacgca aggctggccg ctctggagcg cgtttctgct
     1681 cgcgatcctc ctttccctgg cgatcggctg gctgctcggc taccccgcgt tgcgcgtgca
     1741 gcatcactac ctcgcctttg tcacgctttc gttcgcaacg ctggtgtacc tgatgctgcg
     1801 caacgaagag tggctgacca agggcgtcta cggggtctcc aacatcccgc ggccgacgct
     1861 gctcggctgg tcgacgaagg gcccggtagc gttctacttc ttctgcctcg gggtcctcgc
     1921 gctcgtctcg gcggcgacct ggtggctggt gcgctcgcca tgggggcgag ccttcaccgc
     1981 gctgcgcgag aacgcgatgc gcgccgactc gctcggcgtc gatacgcggc gctacacgct
     2041 gatggcgttc gccttcggct cggcgatcgg cggaatcgcc ggggtgctgt acgcgccgat
     2101 ggtccagttc atcgagccca ccgccttcaa cctcaacctg tccctgaacc tgctgctgat
     2161 ggtgatggtc ggcggctcgg gcttcttcct cggccccttc ctcggcgccg cggtttcggt
     2221 gctgctgccc gagtggatgc gcttcggcag cgagggcctg tacctcatct tctacgcgat
     2281 cgcggtgatg gtgctgatgg cgttctgtcc ttcgggattg ctggggctgg cagaacgcgc
     2341 aagaaggaag ttcggatgaa tgcgccgcct cgcgccgttc tcgaggtccg cagcctcgcc
     2401 aaggcgttcg ggggcatcca cgccgtgaac ggcgtttcct tcgacgtgcg cgaaggcgag
     2461 atcctcggcc tgatcgggcc gaacggctcg ggcaagtcga cgctgttcaa ctgcatcctc
     2521 ggccagctgc cgccctcggc cggcgaggtg cgcatcgacg ggcgcgccat caccgggatg
     2581 cggccctgcc agctcaaccg gctcggggtc ggccgcacct tccagctgct gcaggtgttt
     2641 ccgcagctca ccgtgcggga gaacctgatc ctcgccgggc aggagcacca ggcgtcgatg
     2701 tggtcgcgtc tggtcgggcg gcgggacgcc ggcctcggcc cggcggccga ccgaatgatc
     2761 gagttcttcc ggctggcgca gctcgcagaa gagaaggccg ctgcccttag ctacggccag
     2821 cagaagctgc tcgacgtcgc gatggcgttc atggccgggc cgcgcctcgt gctgctcgac
     2881 gagcctgccg gcggcgtcaa cccgaccatg ctcgccggtc tgcgcgagcg cctgcgcgag
     2941 atcaacgcga agaagcgcgc cacgttcgtg gtcatcgagc acaacatgga gttcgtgatg
     3001 tcgctgtgca cgcgggtcct ggtgctcgcc gaaggccgca tcatcgccga gggcacgccg
     3061 gaggatatcc gcggcaatcc acaggtcatc gaggcctacc ttggcggata gtcttttgat
     3121 ccttgaaaat gtcgtggccg gctacggcgg gctcactgtc ctgaacggga ccagcctgga
     3181 ggcggcgcgc ggcgcgatca ccaccatcat cggcccgaac ggcgcgggca agtccacgat
     3241 cttcaagacc gtgttcgggc tgctgccggc gcgctcgggc cgcgtcgtgt tcgaggggcg
     3301 cgaggtgacc aacctcggcc cgcgcaagct gctcgagctg ggcatctcct acgtgccgca
     3361 ggggcgcaac atctttcccg agctgagcgt gcgccacaac ctcgagctcg gcgcgaccgc
     3421 ggcgcgcgac gggatcgagg tcgcggcgcg gatcgaggcg gtcatggacc gctttccgat
     3481 gctgcgcgag cgcgccgccg cgcaggcctc cacgctctcc ggcggccagc agaagctgct
     3541 cgagatcgcg cgcagcctgc tgctcgaccc gcggctgatg ctgatcgacg agccgtcgat
     3601 cgggctttcg ccgcggctgg tgcaggagac cttcgccatc ctgcaggacc tgcgcgcaaa
     3661 aggcatgtcg atcctgatgg tcgagcagaa cgcgaagcgc gcgctcgagg cctccgacta
     3721 cggcgtggtg atcgagctcg gccgtacgcg catggcgggc gaggcgcgcg cgatcctcgc
     3781 cgacccgaag gtcgggcagc tctttctcgg cggcgggctg gctgctactg gatgaaccgg
     3841 tcgacgcgcg ccagcagcgc cggcggtatc ttcatcccca gcgccttggc cgcggtcata
     3901 ttgaggctca ggctgatgct ttgcgcctgc tcgatgggga tctcgctcgc attcccgcct
     3961 ttcaggatct tgtcggtgaa tctggcgagc gcccggaagc cctccgtgat cgtgggaccg
     4021 tagctcgcca ggccgccggc gtcgacgaat tccgtccagc cgtacatgca gggcatccgg
     4081 gtctcggcgg ccatctccgc gatttccttg cggcgattga gcgtgagcga gtcgggaaag
     4141 acgagggtgg cgtcgaactt cgccgcgcgg atcgccgcga aggccggcgc gagctcctgg
     4201 ggcttgtgca cgaggtagcg agtggtttcg gcacccaggc ggcgcgcagt ctcctccgtc
     4261 acgcggtact cgagcagctc gccggcgtgc tcgggattgg agagaagggc gatgcgcttg
     4321 gcgccgggca gcaggtcctt cagcacctcc acgcgcttgg cgcagagctc catcgccatg
     4381 aacgtcatgc cggtcatgtt gcgcccgggc ttccccaggt tgtctgcgaa tccggcggcg
     4441 accgggtcgc cgctgtagac gaacacgacg ggaacccggt cgcgcacggg cttggtgaaa
     4501 tccaccgaag ggccgcgtga aaccaggaca tcgatgcgat ccgcgacgag ctcggcgacg
     4561 agcgccggca gccgagtgaa atcgttgttg gcgtagcgcg cgctcatcac gtaggacttg
     4621 ccctcgacgt ggccgagctt gctcaagcct tcgcgaagca aggcgagcgc gggatcgggc
     4681 tcggggagat acgagagcca gcccactcgt ggtgggctgc cacgttgcgc cagagaggtg
     4741 cccgaaccga gcaggagcga tccggcgagc caacgcagga ggtcgcggcg attcacactc
     4801 ccagtttatc gcttcggccc gacctggatc ctgattgcaa cgccactcgg gtcgtgtgtc
     4861 agattcaccg gcgccccgac cgcgagctga gagcggttac ccgggacgag ggtcgagacc
     4921 gacgcgcttt caggcaccac gatcttctgc atcccgccct cgtaggtcat ggtgaattcc
     4981 cggccgcgag cggcgccttc caccgattcg acgaccgcgc cgacactggc attggtcatg
     5041 gtctggtcgt cgcggccgtc gaacggccgg tggccgggat tgagcggctt cgggaagcgg
     5101 cgcacctcgt aggcgatcag cttgccgtcg ccgtgcttca ccgaggtgac gccgaggaag
     5161 tcgcccggct tgatgtcggc cagcgccacc ggctgcgtga agacgatctc ggttttctcg
     5221 ccgagtcgga cctgggtctt tccgtccacg gtcaagacat cttctttcaa ggaagaaatc
     5281 gtgccgcgca cgcgcgtcgg cgcctgcgcg acggccacgc aggcgacgaa caggagcgcg
     5341 aggctactga ggcgggcgaa cgccatcttt gctcaccgag atgcgttgcg cgagcagctt
     5401 gtcgccctgc tggccggcga tggtgaagat cgattcgccg ggcttgaggt cggcgcgcga
     5461 gcccgggacg aagaccacga tcggcgtgcc cggcggcacc agaatcttct gcgagccgcc
     5521 ctggtagttc atggtgatct cgttgccgcc gctcgcttgc gcgatgccct cgaggttggc
     5581 gttggtcatc gtcgagtcgg gctgcaggtc ccaggggaaa tgccccggct tcgcctgcgg
     5641 cgggatcgcg tgcacctcga cggcggtcat ctttccatcc ttggtgatcg cagtgacgcc
     5701 gacgtacttg tccttcagct cgtcgagctt cacggacttc gcggtggaca cgcctacgtc
     5761 ggaggcgagg tggaccttga gatccttgcc gtcgcgcgac ttgaccgaca gcacgtcgcc
     5821 gtcgagcgcg gtgatggtgc cgcgcacgcg cgtgggcgcc tgcgcgattg ccagtgaaga
     5881 aacgaatacc agggcgagaa gtgcaaggtg tttcataacg acctccttaa tcgatcttgg
     5941 cgccggagcg cttcaccacg tcggcccact tggccgaatc gcgccggatc agcgcggcga
     6001 attcctcggg tgtgccgccg ccgccctcgc tgccgagctg cgccaggcgc tccttcgtct
     6061 ccggatggac gatcgcgcgg ttcaccgcgg cgttcagctt gtcgagcact gcgcgcggca
     6121 cgccggcggt ggtgatgatg ccgctccacg cgttcacttc gtagccggcc acgccggcct
     6181 cgatcagcgt cggcacctcg ggcaggctga tggcgcgccg ctcgccggtc accgccagcg
     6241 cgcgcacccg gcccgcgcgg acgtgcgatg cgatggagtt ggtgctctcg aacatgagct
     6301 gcacctggcc ggagatcagg tcggcgatcg cgggcgcgcc gcccttgtac gggatgtgca
     6361 cgatgtcggt gccggtcatg tacttgaaca gctcgccgct cacgtggccg ggcgagccgt
     6421 tgctcgagga agcgttggac agcttgccgg ggttcttctt ggcgtagtcg atcagctccg
     6481 ccacgctctg gaagggcgct tttggcgaag ccgcgaggag catgtatccg gtggtcacca
     6541 gcgccaccgg ctggatgtcg cgctcgatgt cgtagggcag cttcgccacc atgtggcgcg
     6601 tgatggcgag cgcgcccacc ggcccctggc cgagggtgta gccgtcggcc ggcgccttgg
     6661 ccaccgcatc gatgccgatg gtgagcgcgc cgcccgggcg gttgtcgacc accacggcct
     6721 ggccgagctc cttggcgagc gcgggggcga cgatgcgggc gatgttgtcg gtcgcgctgc
     6781 cggcggcctg gggaacgacg aggcgaagcg gccgctcggg aaactgcgcg gcggcgggcc
     6841 acgcaattcc cagagcaaga agaaaagcta ggcgcttcag ggcgccagct gcccgcgcac
     6901 ttcgccaccc ttgttgcggt tggtgtgcac gttgacgtac aggttgccgg ccttcaggtc
     6961 gttgatctgc tcgggcgtga gcttgcggcc agcgggcgcg gtataggtat cgccgttctt
     7021 gtcgagaggc acgatcaccg gcccgttgac gccctttgcg ccgcggtgga tgtgggccat
     7081 cgtgccctgc acgtccttgg tcgtgacgct gccggtgatg gtgccgtcct cggcgacgcg
     7141 gaagctgccg gatcccgagc cggggacact gaccggcggc acttcctcgg cgccggtgag
     7201 gctcaccttg atggcgccgc tgcccggcat ccaggcgggc atgctcgagc agcccgcgag
     7261 aacgaccgca gcgattgcca gaaccagttt cactgtcagc ttgctcatga acgcctccta
     7321 gatagaaccg aacacacgga cggctagaat attcccatgc cacggatcgc tgcttctacc
     7381 gcgcttgctc gcctgcgcat tctagacttc tcgcgcgtgc gcgcgggacc gacctgcgtg
     7441 cgccagttcg ccgacttcgg cgccgacgtg atcaagatcg agtcgccgcc cggcgtcgac
     7501 ccgaacgagg ccatgggcgg gccgcgccac ggccccgaca tgcagaacct gcaccgcaac
     7561 aagcgctcga tgaccctcaa cctcaagctg ccggagtcca ggggcgtgct cgagcgcctg
     7621 gcgaagaccg cggacgtggt ggtcgagaac taccggccgg acgtcaaatt ccgcctcggg
     7681 attgactatg cttccctgga aaaaataaat ccaaggatca tcctggcgag catttccggc
     7741 ttcggccagg acggcccgta cgccaagcgc ccgggcttcg accagatcgc gcaggggatg
     7801 agcgggctca tgtcggtgac cgggcttccc ggccaggggc cggtgcggac cggcgccgcg
     7861 atcgccgacc tcaccgctgg actgtatgcc gcgatcggcg tgatgatcgc gctgctcgag
     7921 cgcgagactt cgggcaaggg ccagtgggtg cagagctcgc tcctgcaggc ggggatctcg
     7981 ctgctcgact tccaggcggc gcgctacctc atggagggcg acgtgccgcc gcaggtgggc
     8041 aacgaccacc cgacgagcat gccgacctcg gcctacaaga caaaggacgg gcacatcaac
     8101 gtcgcggctt cgggcgaggg catgtgggcg ggcctgtgca aggctatcgg ccgcgaggac
     8161 atgctcgcct cgccggactt caagggcggg aagaagcgag ccgagaatcg cgccaagctg
     8221 aattcgatcc tgaatgaaag tctttccagg aagaccggcg ccgaatggat cgagatcctg
     8281 aacaaggccg gcatcccgtg cgggccgatc tacaaggtcg acgaggtgtt cgccgacccg
     8341 caggtgaagc acctgggcgt cgccaccgcc gtggagcatc ccaagctcgg ccgcaaggag
     8401 attctggcca atgcggtcgc cctgtcacgc acgccggcca aggtggtcgc gcccacgccc
     8461 gagatcggcg cgcacaccga cgaaatcttg aaggaactca actacaacgc ggcggagatc
     8521 gcgaaactca gagaaggagg agtggtgtga agaagctgat tgcttttgca ggaatacttt
     8581 tctcgggaat ctgcttcgcg cagtatccca acaaggcggt gacgatcgtg gtcggcttcg
     8641 agcccggcgg cggcaccgac acgaccgcgc gcatcgtgca ggggccgctc ggcgagcagt
     8701 tgggacagca agtcgtggtc gagaaccgcg ccggcgccgg cggcaacatc gcggtggact
     8761 acgtcgcgaa gcaggcgccc gacggctaca ccctcgtgct cgccaacgtc ggcgcgctcg
     8821 cggtgaaccc ccatatcctg aagacgccgt acgacccgct gaaggacctg gtgccgatca
     8881 gcatggccgc cgagttcgcc aacgtgctgg tggtgcagcc ctcgctcggc gtgaatacgg
     8941 tcgccgattt cgtgaagatc gcgaaggcga agcccgccac cgtcacctac gcctcgtcgg
     9001 ggatcggcgg ggccggccac ctttccggcg agctcctgcg gcgcatggcg gacatcgaca
     9061 tcgtgcatgt cccctacaag ggcggcgggc cggcgatgca gggattcctc ggcggccagg
     9121 tcgcttcgtt cttcgccacg ccgatttcct cgatctcgca gatccgcgcc ggcaaggcaa
     9181 aggcggtcgc caccacggga tccaagcgcg ccgcgctgat gcccgacgtg ccgacggtcg
     9241 ccgagtcggg ctatcccggc tacgaggcgc tcaactggta cggcttcctg ggtccgaagg
     9301 gcctgccgaa ggagatcgtc gagcggctga accgggagat cgtcaaggcc ctggcgcacc
     9361 cgcaggtggt gtccgcgctg cacaagaccg gcaccgagcc caagagcagc agccccgagg
     9421 agttcgcggc cttcatcaag cgcgagtacg acacctgggg caaggtcgtg aaggaagccg
     9481 gcatcaaggc ccagtagtca gattcgcacg acgagcttgc cgacgtgggc gtcggcttcc
     9541 atgcgtgcct tcgcctcggg cagctcctgg aagccgtaaa cccggtcgat aaccggtttc
     9601 agcttgccac tcgcgaatag cggcaggaaa tctctattga acccggcgac ggtcacgccc
     9661 cgctgcgcgg cgttgcgcat cttgttcgac acgccgaaga gcttgaggcg cttggagtgc
     9721 agcgcctcga tgtcgatctc gctcttcatt acgccgtcga cgtagcccac cgtggcgagc
     9781 cggccttcga agccgagcga cttcacgcac tcggcgaaca ccgagccgcc gacgttgttg
     9841 accaccaggt tcacgccttt tccttgcgtc gcctcgagga ctttggccga gaaatcggga
     9901 tttcgcgtcc ggagggccac atcgaggcct agtgacttca gcttctgcag cttctcatcc
     9961 gagccggagg tgccgatcac cttggcgcca agagccttgg ccgcaagaag cgcggcgact
    10021 cccacgccgg cggatacgcc ggtcacgagg agccattcgt ctttcttgag gcttccctgc
    10081 tgcaccagca tgtcgtagac cacgaggaag gtgatcggga tcgccgcggc ctcttcccaa
    10141 gagaggtttt gcggcaccgg gatcgcgtcc tcggcgtcca tcagcgcgta ttcggcgaag
    10201 ctgcccggca agcggcccat cacacgggcg ccgctgcctt cgacttcgcc agcgccttcg
    10261 gtgccgccga tcttggcggg ccctggcttg gtgaggccgt ggcgcagcag ctcgccgcga
    10321 ttcaatccgg cggcgcgcac gcgcaccaga tgctggccgg gcttcggctg cggcgccggc
    10381 gcgtcgcgca gctcgagctg catttcgggc gatttgagga aatatgcacg catggcgcga
    10441 gtgtatagcg ccacgtgtag caattcggcg acggcgattt ccggtgcgcg cgacagccaa
    10501 gggagcgcgc cttggctaca ttggggtcga aggagatcaa ccccatgatg cagcggctag
    10561 gcaaatggtt cgtggatttc tggatcgtgc tggctttcgg cctggcggcg gcgctgctca
    10621 tgccgccggt gcacgccgag atgatcggca ccgaaagcgt gcaggcgcag gacgaacgcg
    10681 cacgggtgaa ggcgctgctg gagcgcccgg agctcgccaa gggcctggaa aggatgggaa
    10741 tcgcgccgcg cgacgcagtg ggccgtgtcg acgcgatgtc ggacgaggag gtgcttcagc
    10801 tcgccggccg gctcgacgcc gcgctcgccg gcgggcagat gagcaatgaa accctgctgc
    10861 tcatcatcat cgtcatcctg ctgctgatca tcattctcta agcagcgagc tctaccggct
    10921 ccgcgcggtc gtcgaattcg gggacggagc cctaactccg atagctgcgc gtcgccggag
    10981 ttagggctcc gtccccgaat tcctaggcgg cgagctcgat cgcttcgctg cggtcgttga
    11041 tcgtgtgcag ccggaccggg cggcgccatg cgcgccgcgc gtactcgagc accttccgcg
    11101 cgcgccccgc gtccaggccg cggccgtcgc tcgagtgctc gtgcgcgagc accaggccgc
    11161 cgtcgggctt gatctccacc gcggcgacgc gcggcgcgcc gaagttgaac ttgggcgcga
    11221 ggatcgcttc gcgcaccgcg ttgaggtcgc ggttggcgac gcggatctcg tcgttcgggc
    11281 gcgcctcgaa cacgaaaagg tcgagcttct ccgcgagctc gcggtcgagc cagttgcgga
    11341 tgaagccgaa gtcgtcctcc tcgcgcatca cctggcgcgc gcgctcgagg ccgtggcgct
    11401 cgacgatggt ctcccacagg gtgaagccca ggtggtaggg gttgatggcg agcgccgtct
    11461 gctcgcccga ggcgtacggc cgcaccacgt cggaatgggc cttgatcgcc tcgaggtagg
    11521 tggtctgcgg caggaagtcg gcctcgcgca ggaggcgcgc gtgccagtag gacgcccagc
    11581 cctcgttcat gatctggcag gcgaacaccg ggtagaagta gagcgactcg gcgcgcaccg
    11641 cgaggaagat gtcgcgctcc cactgctcga gctcgggcgc gtagttggcg atgaaccaga
    11701 ggaggtcgta ttccgggtgc ggcggcacgc gcgtgcgctg cgggccgagg gtgagcccga
    11761 gcggcagcgc ctcgggccgc gacgcgcctg ggagttcctg gaatctcttc tgaaagtggc
    11821 tggcttgcgc cttcggcttg tccgcgatga actcgggata gcgcgagcgg tgcagctcgc
    11881 cgccgacgtc gatgtgcgac tcgagcgcga gcgcggcgtc gagcacggct tcgacgcgct
    11941 cgagacccgc ggtgtcgatc gcctgctgga tgcgctgcgc gtgcaccgcc gccttctcga
    12001 tgatgttgcc gcccgccatg tcgtggaagc gcgcgaacag ctggttgttc ttcgagaagt
    12061 cggcgtggcc gaccacgtgc gcggtgacca gcgcgttctc ggcgagcgag ttggtgtcca
    12121 tcaggaacgc gcggcagggg tcgccgggga acatcacctc gaaaatcttc gagttcccca
    12181 tgctctggcg cacgagctgg tggatgtagc gcacgccgaa cgaccagtgc ggcatgcgga
    12241 tcggcagccc gtagaccgcg atttcggtca tcaggctctg cggcgccatc tcgaagtcga
    12301 ccgggtagta gtcgaggccg agccggtgcg cgagcgactc gatgcgtccc gcgtaatcct
    12361 cgagcgcggc ggtctcgtca ctcattcgca gcctgctggc tgaagaattc gcggatcgcg
    12421 cgccagatgt cttcctgcga gccgagatgg ctcacgccca ccggcgcgcc gccgttcttc
    12481 agctcgccga acagctccgc gagctgggtc tcgcgcggcc ggaaggtggc gacaccgccg
    12541 gtctcgcagt agccggcgta gttcacgagc ttgcacagct cgcccagcgc gctgcgcgcc
    12601 gcgtctcggt cctcggcggc gttctcgccg tccgacgcgt agaagaagta ggtgttgtag
    12661 cggctcgggt cgaagcgctt ctgcaccgtg tcgagcgcga ggctcgccac gctggaggtc
    12721 accgtgccgc cgctggagga ggcctggaag aactggctct cgtcgaactc ccaggcttcg
    12781 gcggcgtggg cgaggaacat cgtctcgacc ttggtgtact ggcgccggat gccctgcagc
    12841 gcgaagaaga agaactgctt ggcgagccgg cgctgcgcct cgtccatgct gcccgagacg
    12901 tccagggcaa agatgaccgc ggcgttggtg gcgggcgtcg cgcgcttcgc gagctggcgg
    12961 aagcgcaggt cgtcgttgac gatcgcgacc gtctcggttt gaatcgacct tctcttgatc
    13021 gactccttca gggtgcggcg ccgatccagg cgcgagcggg cgccgcgctt gtcccagccc
    13081 tcgcgcacgt agtcgggctc gtcgagcgcg gcggaacgct tgggcttgag ctcggggagc
    13141 ttcagctctt cccatagcca gtcgacgatc tcgtcgatct tcagctccac cacgaacctg
    13201 atctcgccgt ttccggtgcc gccggcgcgt ccctgagagt cgccgtcctc gcgcccgggc
    13261 tgcagcacat cgcccggctg gcccttgccc tggccggcgc cctgttgagc gtctgcgtcg
    13321 agcaggcgaa agcgcgagtg ctcgagcatc ttcaccggca ccgcgacggt gcggttgccg
    13381 gggcgggtga tgaggtcgcc ccccgccagc agctcgggca ggctctcctt caccgccgag
    13441 cgcaccttct ggttatgccg cagccagtcc cgggcccctc gggaaaagag gtcgtaccag
    13501 ggtgtttccg cggcaattcg gagggtcact cttgcgccaa tagggtcgtg acgtagttga
    13561 gggcctcgcg ggcgctgtgc gagtcgtagc cgtactcgtc gaccaggcgc ttctcgacca
    13621 ccgagatctt ctggcgcgtc tcgtcatcgg ggcgcgccga ggagctcacc aggcgcagca
    13681 cgtcgcggcg ctgctcgaag aggtactgct cgaccgcgtc gtgcagcggc gcgtggctgt
    13741 cgagcgtgaa cttctcgccg cgcttgtacg cgcccatcgc cttgcgcacc acttcctggc
    13801 ggaacgagag cttgcccgac tcggagatgc ggatcttttc ctcgacgttg cgcaggaagc
    13861 gctcgtcggg cttgcgttcc tcggtggtga tcgggtcctt gagggtacgg ttgtcgaggg
    13921 tcgcctcgac ctcgtcgagg tacttgtcga gcagctgctg cgcctcctgc tcgaaggaga
    13981 cgaacagcgc cttgtgcacg tcctccttga cccagcggtt gtagaactcc ttgcgcaccg
    14041 tgaccaggaa atcgacccac ttgcgcttct tcttgccgtc gatgcgcacg tcggcctcga
    14101 tcgcgtcctt gagcgcgatc aggacatcca tggtcgtcag gctgctcttc gcggactgga
    14161 tgatggcgct cgagagcgcg ttgatcacga agcgcggcga cacgccggcc aggccctctt
    14221 cgggattgcg cgcgcggatc ttgttcacct cgttgcccga cacgccgtcg acgtcctcgc
    14281 cggcgtacag gcgcgccttc ttggtgccgt cgctttcgcc ctcgctttcg gcgaggcgcg
    14341 tcagcaccga gaacgccgcg gccacgtgca gcgcgtgcgg atcgaggtgc acctcgcgga
    14401 acgcctgggt ggaggaggtg agcttgcggt agatcttcgc ctcttccttg taggagaggg
    14461 tgtagggcac ctggatgatc accatgcggt cgagcagcgc ctcgttctcg ggctcctgca
    14521 ggaacttgcg gaactcggcg aggttggtgt gcgcgaggat ggtctcgtcg aggtggatca
    14581 gcgggaagcg cgacaccttg acgttcttct cctgggtgag cgtgagcagg agatagagga
    14641 attcgcgctt caccttgagg atctcgatca tctcgagcgc gccgcggctt gcggcgaaca
    14701 ccgcgcccga ccacgaccag gcgcgcgggt cgccttcgtc gccgaactgg gagaccttgg
    14761 agaggtcgac cgagccgacc aggtcggcga tgtccgcggt ggtcgggtcg tgcggcgcat
    14821 aggtgccgac gccgacgcgc ccggcctcgg agatgaagat gcgctgcacc ggcatctgca
    14881 tgaagtcgcc gtcgaactcg tgctgcaggc ggctggtgca gtacgggcag agctcgcccg
    14941 cgatgtcgac gccgtaggtc tggcggaact gggcgcgcat ggtgtgcggg accagatgca
    15001 ggggagactc gtgcaccggg cagccgtcga tgccgtagag cgcccccgcc tcggtgtggc
    15061 tgtaggcctc caggccgcgc ttgagcagga tcaccatgct cgacttgccg cccgagggcg
    15121 gcccgagcag cagcaggagc cgccggccga cctcgctgcc ggcgctcgcc gccttgaaat
    15181 actccatcac gcgctcgagg gtgtcgtcga tgccgtagag ctcgtcctgg aaaagggtgt
    15241 ctttcgagcg catcatgtcc cacatgtact ggtggctcga gcgcgcgatg ccgcgcgaat
    15301 cggcggggag cacgctttcc aggaaggctg cgaagctccc cgtccagttc gccgcgcggt
    15361 gctgcttggt gaaatcgctg agggagcgga tgagattcga acgccccgct tcgcgcgtct
    15421 cgtcgttctg gctgatcgct cgtaccggca tgttcatggt ctcgcctcca tttccttcag
    15481 atgctattaa ccaccaacta gtgcccgtga actaccctcg aaagcaagca acgaacggtc
    15541 caacgggtct acgaaaaatt agttgacgta acaaaatgaa aacgtgccgg gaggtttccc
    15601 tacacgccgc gcggaatcgt cgcagccgcg agaactggcc agcgaccgcg cgtcatgatt
    15661 caatgacagt accgatgaac gcggactcgg tcataatcgt tgactagccg acatgcttaa
    15721 agaaatcctc ggggtcgccg acgacccgcc agccaagagg cgctggttcc acgatgagta
    15781 tttcgacctg ttcgtctggc aggcccaggg cgaggtgacc ctgttccagc tctgctacgg
    15841 cgaggacgct accgagcgcg ccctggtctg ggacaaggag cgcggcttct tccacgacgg
    15901 tcccgcatcg cgggatgtgg ttgcgctatt cgaagacgcc gcaaccgcgc tgccccccga
    15961 cctcgccgcc gagatccgcc agaaggtgca cgagttcgcc ggccgccagc tcacgatcgt
    16021 gtcgcgccgc aagaaattcc gccgcgccgc ctggcagcgc tgaaaagcac gtttgtagcg
    16081 gttggatgac aatccctcct ttcgggaggg aataacaatg aaaccggaac tgatgtggct
    16141 gctgtgggcg gtcgcgctca cgttcgcgca gatgcttgtc gcagtgggcg gggcgacgct
    16201 tcaagtgggg ctgccgatgc tcgccggcaa ccgggaagga ctcgccccgt gcaccggctg
    16261 ggccggccgg gcgcagcgcg cgcaccacaa catgctcgag aacctggtgc tccttgccgc
    16321 gctcgtcctc atggcagtcg tttcaggaaa gaccaatgcc atgacgctgc tcggcgcgca
    16381 gcttttcttc tgggcgaggc tcgcctacgc cttcgtatac ctcgccggca ttccctggct
    16441 gcgcaccgcg gtgtggctcg tctcggtgat cgggctgctc ctcattttcg tccagctctt
    16501 ataggaggca caccgatcgc gaccgggcgc accgccgacc cggcggcgcc ctggtcgcgc
    16561 agcggcagga tcagcgtgca aaaattccac acgcggtcgg ccgccagctc gtcgagcttc
    16621 gcgttctcga ccaggtggat tccggcctgc gagagcatgt gatggtgcac cgcaaacggc
    16681 agccccgggg gcgtgcccgg cgccgggccg gccttgccca tcagcatgcc ttcggcgacg
    16741 gtgtcgacga acggggtgtc gagcgcgatc gccacgatgc gcttctcggc aagccactgc
    16801 gcggcgtcga aggacaggcc gggggccttg gtgtagtaga acttctcggt gtcggggtcg
    16861 cgccagtggt cgccccagcc ggtgcgcacg tacaccacgt cgcccggcag cacgccgcgc
    16921 ccggcaagcc cctgcgcctt cagcatgcct tccaggtcct ttgccgagac cagctcgccg
    16981 tccttcatcc cggctttctt gtatgccttt gcatcgagca gcacggcgct ggtgatgatc
    17041 ggcggggcct tgtcgatgcc gagcttgagg agcggcgaat cgggcgtggg cttgacgtcc
    17101 ttctgcgtga agccgccgta gtacacggct tccccggccg gaaactcggt cttgccgtcc
    17161 cacggctgct tgaaggcgcc gaagtgccct agcgcgtcca tctgcgtgcc ctgctgggcc
    17221 ggttccgcat tctcattcat ttgttctgaa ttgaaggcgt gcgcggtacc cggaatgccg
    17281 gcgctcgcct tcggcttgac cgcgtaaggc ccggcgaacg gcgagagcgg catggtgttc
    17341 gagcgcaggt aggacagctc gtacgacctc gcgcccggct gctgcatgtg ccaggcgcag
    17401 cgctggaacg tcgccggccc gatctggttc gccatgccgc gctcgtcgcc ctgcggccag
    17461 ggcggcggcg gcaccctcag gcgccgcagg ttgtcgggct ccgtcgcctg ggccgagaac
    17521 gcggccagca gcagcatcag atgaatcggt ttcacttgag gccctcctct gcgagttgtg
    17581 ccagcgtgct gcgtcccttc agcatgtcgt ccaggctgcc gaaggcctgc atgtagtggc
    17641 cgcgatagcc gctcgcctcg atggcggcga acatcgcgcc gaagtcgatc gtgccttcgc
    17701 ccgggcgcag gtgctcctcg tgcgtgccgc ggcagtcagc gaggcgcacc tcgccgcagc
    17761 ggctcatgtc gaggccggcc atgaacgcgt cgatgcggtt cgggcggatg tgcgcgtggt
    17821 tcacggtgaa gctccagccg agcgcgggcg aatcgagccg ctcgaaatag tagcggcact
    17881 cctcgaggtc gttgcagagg taatgcacct cggcctgctc gggctcgggg ttcaggttct
    17941 ccagcaacaa cttgactttc ttcttctccg cggccttggc cgcgcgcgcg atgcgcgcca
    18001 cggcagcgtc catgcgcgcg cgcttgtcgc cgccaaagtg gtagccgccg tgcaccacga
    18061 cccagccggc gccgagcttc ttcgcggcgt ccaggtacgc ggcgaggtag gcgtcggccg
    18121 cgctggtgac gaaaggagag gtctcggcga tgttgaccgc cgagagggtg tgaagaccga
    18181 gcttgatgtc gttcgcttcc aggtgctttc tcgcgttcgc cagccgcggc gagctcttct
    18241 cgagcaaatc cggggtctgg tccaggcaaa gatcgatcac cggcaccttg tgctcgatcg
    18301 cccacgcgat cgcgcgctcg agcggcagct tctggttgat gtcgacgcct atgcggtcaa
    18361 agaattttgg cacggaagaa cctgctccaa aggggggcgg taaccgcgat gagcgcgaac
    18421 gcgaccagca cggcagcgat cgggcgcgtg acgaagatca gcccgctacc gtgcgagagg
    18481 gtcagcgact ggcgcagcga gttctcgaag atcggcccga gcaccaggcc gaggatcatc
    18541 ggcgctgccg accagtcgta acgcttcatc agccagccca gcacgccgaa gccgagcatc
    18601 agccacgcat cctcgacgtt gttcgcgcgc gagaccacgc cggtgacgca gatcgcgagg
    18661 atcggcgggt agatccagct gtagcggatc tgcagcaggc gcgcgaacag gcccgcgagc
    18721 ggcaggttca tcaccaggag gatcaggttg ccgatgaaca tgctggcgat caccgcccat
    18781 accagcgccg cgttggtggt gaacatgtcc gggccgggac gcaggccgaa catgatcagc
    18841 gccgcgagca tcaccgcggt ggtgttcgac cccggcacgc cgagcgcgaa catcggcacc
    18901 atggcgccgg ccgaggccgc gttgttagcg gattccgggc cggcgacgcc ctcgatcgcg
    18961 cccttgccga agcgcgacgg gtccttcgcc agcttctttt cggcgatgta gccgacgaag
    19021 gacgcgatgg tcgcgcccgc ggccggcagg atgccgatga ggaagccgat gccgctaccg
    19081 cgcaggatcg ccatgcgcgc ctgccgccac tcctcgcggt tcggcagcac gcgtcctacg
    19141 ttcagaatgg gagttgccga gcccatccgg cagctggcga ggacctcgcc gacgccgaag
    19201 agcgcgaccg ccaggatgat gaagtccacg ccgtcgagca gccagagctg cccgaagtcg
    19261 aagcgcggcg cgccgacctg cggatcgatg ccgatggtgc cgaggagcag gcccgccacg
    19321 ccggtcgcga gccccttgag gaccgagccg ccgacggcgg cgagcgccat caggccgagc
    19381 agcacgagcg cgaaatactc gggcggcccg aacgccagcg cgaccttggc gagcggcggt
    19441 gcgagcagca tgaggccgac cgtgccgaga cacccggcga tgaaggagcc gatggcggca
    19501 atgccgaggg ccacgccgcc gcggccctgc agcgccatct ggtagccgtc gagggtggtc
    19561 atcaccgagg tggactcgcc cggcacgttg atcagcaccg aggtgagcgt tccgccgtat
    19621 tgcgcgccgt agtagaggcc ggcgaacatg atgatggcgc cggtggggct ggcgccgtag
    19681 gtgagcggca gcagcagcgc gatcgccgcc gaaggtccga tgccgggcag gaccccgatg
    19741 atctggccca gcaccacgcc gagcaggcac atcagcaggt tcgccggcgt cgccgccgcg
    19801 aggaacccct gcgcgaggtg gccgagcagt tccatcagat gcccgtcaga tccctaaagg
    19861 cccgaccggc agcggcacgc gcagccaggt ccggaagatg aggaacagca cggcggtagc
    19921 ggtcgtcgcg actaccagcg accggaccat cggcaggcgc tcaaccgccg cgaacagcac
    19981 cagcaggagc aggaagatcg agggggcgaa gccgaccagg tccagcccgg cgatcgaggc
    20041 ggccagcgcg gccagcacga ccagcggctt gccgatcggc tcctccttct ggccggggtc
    20101 cctgcgcagg aacaggttgg cgaggatcgc cgcggccagc gcgacgagga tgatgccgta
    20161 ccagagcggc atgaaccccg actgcggcac gaaccccgcc cacaggggca tgcgcgccgc
    20221 ggccacgatc cagaggatgc cgagaaccgc gaacagcagg gcgagcagca cctcgccggg
    20281 tgccggccgg ttcagcatct ggcgctgcgc cctacttgcc gagcaggtcg cggtagagac
    20341 gctcctgggt cgccaggaac ttctcgaact cggcgccggc gatcgtcgcc tcggaggcgg
    20401 cgttgtcctt gaggtaggcc ttgaactgcg gcgaggccac caccttcgcc atcacgcctt
    20461 cccagtactt cgccgcgtcg gccggcacgc ccttcggcac gccgacgccg cgccacatct
    20521 ggaaggtcgg cgcgttgatg ccctgctcct tcatggtggg gacgttgggc agcatggcga
    20581 agcgcgtgtc gcggaacacg ccgatggcgc gcaccgtgcc cttctgcagg tgacccatga
    20641 actcgagcgg gttaccgagc gaggcatgta cgtggccgcc gagcagcgcg gtcagtgcct
    20701 cgccgccgct gttgaagctg atcatgttga gctcggtgcc ggtcgacttg gcgagcaccg
    20761 tcaccgccat tacgtcggtc gtgccgccgg tggagaagtt gtaggtcttc gccggcttgg
    20821 acttggcgtc cttcacgaac tcggcggcgg tcttccacgg cgcgtcgccg cgcacgaaga
    20881 acacgaagtc gtcgagcatc acgttcatca ccgggtgcac ctcggtcagc gtcttcgccg
    20941 tcggggtgat gatcggcgtg atctgcgtcg agccgttgat caggaccagc gtgttcgggt
    21001 cggccttgcg gctggcgctc atgtagccga tgcccgccgc gccgccgccg ccgggtttgt
    21061 tcaccagcgc cacgggcacc ggcaccagct tgtcttcggt gatgatcttg tggatcacgc
    21121 gggccatcag gtcggcgccg ccgccgaccg cgaacgggat cacgaactcg acatccttgg
    21181 tgggcttcca gccctgggcg gacgccgcga ccggcagagc cgccaggagc agcagttttg
    21241 caaaaaagcg catcgaactc ctcctcgaaa gacgttgttg tgaggcgaac tatactcgcg
    21301 gccatgggca tgctggtaga gggcgtgtgg caggacatcc cgcgcgacac caaggcgacc
    21361 ggcggcgcgt tcgtccgggc ggaaagcgcg tttcgcgatt gggtgagcga gcccgaagcc
    21421 ggccgctatc gcctctacgt tgcgaaaagc tgcccctggg cgcatcgcac gcttgtcgcg
    21481 cgcgcgttga agggcctgga aaaggccgtg ccggtgtcct atgcggatcc ctacatggga
    21541 gagaacggct ggcagttccc cgagggctac ctccacgagc tgtatgcaaa agcgaaaccg
    21601 ggctataccg ggcgcgtgac ggtgccggtg ctgtgggacg agaagcaggg ccgcatcgtc
    21661 aacaacgaat cctccgagat catccgcatg ctgaaccgcg agttcgacgc gctcgcgcgg
    21721 cgcaagctcg ccgatctcta tccagaaaaa cgcagaaggg aaatcgattg gctcaacgaa
    21781 cgcatctacc gcacggtgaa caacggggtg taccgcgcgg gcttcgccac ggcgcaggac
    21841 aagtacgagg aagccgtgag cgggctgttt cgcaccctcg actggctgga gaagcggctg
    21901 gcgaaacgaa agtggctttg cggcaacgac tttacggaag ccgacgtgcg gctcttcacc
    21961 acgctcgtgc gcttcgatgc ggtgtactac tctcacttca agtgcaacct gcggcggctc
    22021 gtcgactacc cgaacctgtg gcgctggacg cgccgggtgt acgcgctgcc gggcgtggcg
    22081 aagaccgtcg acatggcgca gatcaagtcg cactactacc gcagcatgaa gcacatcaac
    22141 ccgacgcaga tcgtgccaaa agggccgttg ctggacttct cgctgcacta gttgcattca
    22201 acgtatatgc cacgagcgac cccctcgacg gggtgctcgc gcccggcgag gcggggatcg
    22261 gcttcacctg gcgcttcgag ccctcgcctt accgcggctc ggaccgcgac tcggacctga
    22321 tgatgcagtt ccggtacgac agccgctatt tctacctgca gtcggatcgg atcggcctca
    22381 agctcgaaag cgagcgttac cgctacgagc ttttcctgcg ccgtcgcctc gagggcttcg
    22441 cctccgacca cgtgcccgaa agcatgaccg gcatggcgaa gcgctcgtcg ggcgacgacc
    22501 tcggcgtcgc ggcccgctat cgcctcggcg ccggcgtcgt ttacggcgag gcgatgcaga
    22561 acgtgagcga ggaatcggac ggcgccgagc tgcgcctcgg ctaccgctac gagggctggt
    22621 ggagcggaaa gttgcgctgg cgaccctacg cgaccctggc ctggcgcgat tcgaagctga
    22681 acaactacta ctacggcgtg cgcccggaag aagccaccgc ggagcggccc gaatacaagg
    22741 caggttccgg attgaatctc gagctgggcg tgctcgccgc gtaccggttc gccgagcgct
    22801 ggcagctgct cgcgggcgcg ggcatgacgc gctggtcgag cagcgtgcgc ggcagcccgg
    22861 tggtcgagga cggcctgcag ccttatgcga tgctcggcct gatgtacggc ttcaagcccg
    22921 accagtcgag cgaatgggac cgccggccgc tgatcatgag agtcggctac gggcagtcga
    22981 gcgattgcga cctgacgccg atcgtcacct tgcagtgcac cagcgtgaat acgcaggacg
    23041 acaccagcat cgtgtcgctc gaggtcggcc agatcctggt gcgcgggctg aacggctggc
    23101 ctctcgacct cgcgggcttc gtcggcttcc tcaagcacga ggagcgcggg ctgcaggagg
    23161 actcctggca gatcaacggt tacttcaagg cctactacta cctcggctcc tggcgcgagt
    23221 ggctgcgcac gcgcctcggc ctcggcgccg gaatttcgta tgcgagccgc gtccccttca
    23281 gcgagcagcg cgaccaggcg ctgcgcggcc gcgacacctc gaagctgctt ctgtacctcg
    23341 atccgaccgt cgacatcaac gtgggtgacg tcttgcgcgc gcgcgcgctg cgcgagacct
    23401 atgtcggcct cggcgtttcg caccgctcgg gcgtcttcgg cgcgtcgcgc ctgttcaaca
    23461 gcgtgaacgg cggctcgaac tacatctacg gctatgtcga gacgagcttc tagcctgctc
    23521 ttagtcctgg tcgcgctcgc ctggctcggc tcgcttgcgc tgcggccgct gtacaagccg
    23581 gacgaaggac gttacggcga aatcccgcgc gagatggcgg cgagcggcga ctggctcacg
    23641 ccgcggctga acggcttcaa gtacttcgag aagccgccgc tgcagtactg ggcgaccgcg
    23701 gcggcgttca aggtgttcgg cgtcagcgac tgggccgccc ggctgtggac cggcctcgcc
    23761 gcgctcgccg gcgtcctcat ggtgttcgcc gccggcaacc ggctgtacgc cccacccgcg
    23821 ggcgctttcg ccgccgcgat tctcgcctca agccccctct atgtgctgct cgggcagttc
    23881 aacacgctcg acatgagcgt caccttgttc ctttcggccg cggtgttcgc gttcgcgctc
    23941 gcgcagcgcg ccgcgtccga gcctgccgtg cggcggcgct ggatgctcgc cttctgggcg
    24001 gcgtgcgcgg ctgccgtgct gagcaagggc ctgatcggca tcgtgctgcc gctcggtgcg
    24061 gtggcgcttt acgtgcccgc acgccgcgac tgggcgctgc tgcgccggct cgagctggtg
    24121 cgcggcgggc tgctctttct tctcatcgcc gcgccctggt tcgtcgccgt gtcgcgggcc
    24181 aactcggaat tcgcgcactt tttcttcgtg caggaacact ggctgcggtt cactaccagg
    24241 atgcaccacc gagagcatcc cgcgtggttc ttcctgccgg tcctcgtggc gggcatgtgg
    24301 ccctggctgt tggcggtcat cgccgggtgg gtcgcggcgc tgcggccagc gcccggaaag
    24361 gagttctcgc cggctctttt cctcggtctc tgggcgctgg tcgtgttcgc tttcttttcc
    24421 ggctcgggct ccaagctccc gccctacatc ctgcctctcg ttccggcgct cgccctgctt
    24481 gccgggcggt tgctcgcgcg cgggccccgc agcgggctgc tgatggcgca atcgctcctc
    24541 gtggcggcag ccgcgatggc tactggcggc ggagcctggc gcctcaccgg ggcgggggac
    24601 ggagcgctgt acggtcttgg cgatgactat gctccctggc tcgccgcggc ggcgacgatc
    24661 gggctcgcgg gcgccgtcgg cgcggcggcg ctggcggcgc tcggacgcgc cgcgggcgcc
    24721 gtggcaagcc tcgcggcggg cgcgcttgct gcccttctcg tcgcctccgg cgggcacgcc
    24781 gtattcgcgc ccgcgtacag cgtggcgccg ctggtcgctt ccctggcgca agctccgtct
    24841 cgcgacgctg cgatcttcgc ggtccaggcc tacgaccaca ccatgccctg ggcgctgcgc
    24901 cgcaccgtga ccatggtcgg gtacaaggac gagctggagc aggccgtcgc ctgggagccg
    24961 gaccggttca tccccgacct cgcggcgttt gcgcgcgcct ggaacgccga gcgcgaagcg
    25021 tatgcgttct tcgcggcgcg cgacttcgag cgcctgcgcc gcgagctcga tttgccgatg
    25081 gaaatcgcgg cgcgcgggcc gcgttacgtg atcgtgagga agccatgacg ctcttcagct
    25141 tttggttcat tctcgccggc gtattgctga atgccgctgc gcagcttctg ctcaaggccg
    25201 gcaccaatgc gatgcccctc ggtctccggc tcgccctcga gccgcacatc ctcgccggcc
    25261 tggcgtgcta tgtcgtcagc gtggtcgtct gggtcgtcgc gctctcgcgc gtgccggtga
    25321 gcatcgctta tccgatgctg tcgatcggct acgtggtcaa cgcgatcgcc gcatgggcgc
    25381 tgctcggcga ggcggtcacg ccgatgcgca tggcgggaat cgctgttatc gtcgcgggcg
    25441 tgttcatcgt ggcgcgctcg tgagcgcgtt tcttcccttc gcgcggcccg agatcgacga
    25501 gcagacgatc gcggcggtgg ccgaggtgct gcgttccggc tggatcacct ccgggccgca
    25561 ggtgctgcgt ttcgaagagg cgctgtcggc gcactgcggc ggccgcccgg tgcgcacctt
    25621 cacctcggcg accggcgcgc tcgaggtggc gctcgccctc gccggggtcg ggccgggcga
    25681 cgaggtaatc gtgcccgcga tgaccttcgt cgcgagcgcg aacgtggtgc tgcgcgcggg
    25741 cgcgcggccg gtgctggtgg acgtcgacct ggacagccgc aacaccgatg cggcgcgcat
    25801 cgaggcggcg atcacgccgc gcaccaaggc ggtgatgccg gtgcacttcg ccggcctgcc
    25861 ggtggagctc gcgccggtct gcgcgctggc gaagcgtcgc ggcctgcgcg tgatcgagga
    25921 cgcagcgcac gcgatggggg cgagccacgc cggccgcgcc atcggcgccc agggcgacct
    25981 ggtggcgttc agcttccacc cgaacaagaa catcaccacc atcgagggcg gagcgctctc
    26041 cttcgccgac ccggccgagg tcgagcgcgc cgagcggctg cgcttccacg gcatccgcaa
    26101 gctgcccggc ggcgagatgg aggtcgagga gcccggcggc aagttcaatc tggccgacgt
    26161 tgccgcgtgc atcgggctcg cccagctcgc caggctggcg caattcaacg cgcggcgcag
    26221 cgagctcgcg cggcgctatc tcgaattgct gcagggcatg cgccatgtgc ggctgccggc
    26281 ggccgaccgg cccgggcatt cgtggcatct gttcgccccg ctcgtcgact tcggcgcgct
    26341 cggcatcacg cggcgcgcgc tcatcgaacg gatgcacgcg cgcggcatcg gcgccggggt
    26401 gcactaccag gcgatgcatc tctttgccgc ctaccggcgc ctcggctatg gcccgggcga
    26461 ttttccgaac gccgagacga tcggcgcgcg taccgtgacg ctgccgctct tcacgcagat
    26521 gcaaaacgcc gacgtcgagc gggtgtgtgc cgcgctccaa gcggctatac tggggcgcga
    26581 ataagcgaat ggcggccgcg cccaaagtat cgatagtcgt cccgatctac aacgaggaag
    26641 ccggcctgcc ggcgctgctc gcgcgcctgt atcccgcgct cgacgccctc ggccagacct
    26701 acgagctcat cctggtcgat gatggcagcc gcgaccactc gatccgggtg ctgcgccagc
    26761 aattcgatcg ccgacccgac gtcacccggg tcgtgatcct cgccgccaac gtcggccagc
    26821 atatggcgat catggccggg ttccagcacg cccgcggcga ggtcgtggtg acgctcgacg
    26881 ccgacctgca gaacccgccc gaggagatcc acaagctgct cgccaagatg gaggaaggct
    26941 tcgattacgt cggcagctat cgcagcgagc gccgcgacaa cctgttccgc cgtgccgcct
    27001 cgcgcgccat gaaccggctg cgcgaagacc tgacccgcat ccgcatggcc gaccagggtt
    27061 gcatgctgcg cgcctacgcg cgcaacgtgg tcgacgcgat caaccagtcg cccgaggtga
    27121 acaccttcgt gccggcgctc gccttcatct atgcccgcaa cccgaccgag atcgaggtgc
    27181 gccacgacga gcgcgcggcc ggcgcctcgc agtactcgct gtcccggctg atcaagctca
    27241 acttcgacct ggtgaccggc ttctccgtga agccgctgca gatcttctct ggcatcggca
    27301 tgatcgtggc cgtgggcgcg gtgctgctat acgcgaccgt gctggcttac cagctgtaca
    27361 cggcgggact ggcgggcgcg ctggagcacg tctgggaccg cgacgtgctc gagttcttcc
    27421 tgatggggct cgccgtcttc ggcatcggtc tcaccggcga gtacatcggc cgcatctacg
    27481 agcaagtgcg cggccggccg cgctacatgg tgcaggccgt cctcgagcag cagccgggcg
    27541 ctcagcagtg gcagcccagg cagtcgtctt cgcgtatcac gacgtaggcg tacgctgcct
    27601 gcgcgtgctg ctggaggcgg gcatcgcggt gccgctcgtc gtcacgcacc gcgatgatcc
    27661 ggcggagcgg gtctggttcg cgagcgtcgc cgagctcgcc cgcaagcacg ggatcgagac
    27721 gctgcaggac gccgaaacgg cgcagcctct tgaaagactc cggacccttg gcccggactt
    27781 catcttctcc ttctactatc ggcgcatgct ggcgccgggg ctgctcgcgc tggcgcggcg
    27841 cggcgcgttc aacatgcacg gttcgctgct gccgaaatac cgcggacgcg cgccggtgaa
    27901 ctgggcggtc ctgatgggcg agaccgaaac cggcgcgacg ctgcacgaga tggtcgccaa
    27961 gcccgacgcc ggccgcatcg tcgaccagga gcgcgtgccg atcggccccg acgatctcgc
    28021 cgtcgaggtc atgggcaggg tggccgttgc ggcggaagtg gttttgcgcc gctccctgcc
    28081 cgctctcctt tccggaaccg cgcgcctgcg gccccaggac ctttcccgcg gcagctactt
    28141 cggcggccgc aagcccgagg acgggcgcat cgactggtcg aggagcgcgc gcgagatcca
    28201 caacctggtg cgcgcggtcg cgccgccgta tcctggggca ttcacccatg gaatgaagat
    28261 caaccgaacg aggatcgaac cacaacggca ggcgccgcgc ggcgcgctcg gcccctatcg
    28321 cgaaaacggc gaatggttcg cggcgtgcgg cgatggtaga gtcctgcgcc tcctggagat
    28381 cgagcccctt caatgaagcg cctgctgatc ctcggcgtca acggcttcat cggccaccac
    28441 ctgtccaagc gcgtgctcgc gtccaccgac tgggaaatct acggcatgga catgcagagc
    28501 gaccgcgtcg aggacctgct cggcgagcgg cgcttccact tcttcgaggg cgacatcacc
    28561 atcaacaagg agtggatcga gtaccacatc cgcaagtgcg acaccgtgct gccgctggtg
    28621 gcaatcgcca cgccctcggt gtacgtgaag gacccgctgc gcgtgttcga gctcgacttc
    28681 gaggccaacc tgccgatcgt gcgcgcctgc gtgcaccatc gcaagcgcct gctgttcccg
    28741 tcgacctcgg aagtgtacgg aatgtctccc gatcgcgagt tcgacccgga agactcgccg
    28801 ctggtgtacg ggccgatcag caagcagcgc tggatctacg cctgcgccaa gcagctgatg
    28861 gaccgcgtga tctgggccta cggcgacaag gtcggcctcg acttcaccct gttccggccg
    28921 ttcaactgga tcggcgcggg cctcgattcg ctcgccaccg ccaaggaagg cagctcgcgc
    28981 gtggtcaccc agttcctcgg ccacatctcg cgcggcgagc cgatccagct ggtcgacggc
    29041 gggcgccaga agcgcgcctt cacctacatc gacgacggca tcgatgcgct gatgcggatc
    29101 atcgacaacc cgcgcggcgt cgcctcgggc aagatctaca acatcggcaa tccgcgcaac
    29161 aacctgtcgg tgcgccagct cgccgagatg atggtcgagc tggcgctcga gatccccgag
    29221 taccagaagg ccgcgcgccg ggtgcggctg cggcgcacca ccgccgagcg ctactacggg
    29281 cgcggctacc aggacatgca gaaccgcgtc ccgaaaatcg acaacacgaa acgcgacctg
    29341 cgctggcatc cgcgcgtcgg catgcgcgac gcgctacgcc gcattttcga ggcgtatcgc
    29401 accgagatcg cggaagcccg ccgcctggtc ggatgaagct ggcgttgaag gtggacgtgg
    29461 acacctggcg cggtacgcgc gacggggttc cccggctggt cgagctcctg aagcgccacg
    29521 gcgccggcgc gacgttcctg ttcagcctgg gcccggacca caccgggcgg gcactgcggc
    29581 gggttttccg tcccggattc ttttccaagg tcagacggac ttcggtcctg cggcactacg
    29641 gcctgggcac gctgctgtac ggcaccctgc tccccgggcc ggacatcggg cggcgcggcg
    29701 cgtccaccct gcgcgccgcg cgcgacgccg gattcgaggt cggcatccat tgctgggatc
    29761 acgtgcgctg gcaggatcac gtggcgctcg ccgactcgga ctggaccgag cggcagatgc
    29821 gacgggcgcg cgagcgcttt acggcgatct tccgggaaat accgaggacc tggggagcgg
    29881 cgggctggca gacgaaccgc cacgccgcct gggtcgagga gcgcgagttc gactacgctt
    29941 ccgacacccg cgggcggggc ccgttccggc cgctgtggga cggcgtcgcc atcggctgcc
    30001 cgcaacttcc gaccacgctg ccgaccctgg acgagctcat cggggtggac ggcatcgacg
    30061 aaggcaacgt cgccgcgcac cttctcgatc tcaccgcgca gaagagcccg catgtcttta
    30121 ccgcgcacgc cgagctcgag gggggcgcgc tgctcggcgc cttcgaggcg ctgctgcgcg
    30181 gctggaaagc gcagggctac gagttggtcg cgctgcgctc gctgttcgag tcgctcgacc
    30241 tcgcgcagct tccccggcac gacgtggtgt atggagaaat tccaggccgc acgggcaagg
    30301 tggcgctgca ggcatgatcc tgcgcggcgt tgccctgttc cttctcctga tggcatcggc
    30361 gcatgccgag ctgccgccgc gcctgcagat cacctacgac gtgctgcgca acggctcggc
    30421 ggtcgccgag atcgtcggcc gcctcgagag cgacggcaac acctaccggc tcaccgagag
    30481 ctggaagggc aggagcttct acgcgctgct cggccgggcg atgcgcacca gcgagggtag
    30541 cctcaccccc gcgctggtgc ggccgcgcga attcaccgac gagcgcagcg ggcgcgacac
    30601 cgctcgcgcc tggttcgact ggggcgcgaa cgccatgacc ctgcgctaca agggcaaatc
    30661 ccgcaccgag ccgatcccgc ccaacgcgca ggaccggctg tcgttcatcc tcgctctctc
    30721 ggccgcgccg gcggacgcga agcacggcga ctatcacctg gtcgacggcc gcggcgtctc
    30781 gcaccacatc tacgaattcg ccgggcgcga gcgcgtgcag actccggcgg gggagttcga
    30841 cgcgcgcaag atcgtgcgtg gcgcgaagga cgacgaccag gtccagatct ggctcgccaa
    30901 gcccctgggc ggcctgccgg tgcgcatgct ggtggtcgaa aagggcggaa cccgctggga
    30961 tcaggtcgcg acgcgcgtcg agcgctagcc tgccgtgcga ggacgacgcc cgagacatta
    31021 acgctgcctt aacgcgccac cggatagagt ggcccgagat tgtcgaatca cagggtggcg
    31081 aatgaatgcg ccggggaaag tcgcagtcgg ggacgggatc gccgcgggga atgcgcgctg
    31141 gacctttgcc ggcgatgtcg cccggacgtt ctccgcgcat gtcagcaagt cggtgccgct
    31201 gtacgacgag gggcattacc tcatctgcgc gctttccgac ttcttcgtgc aggactcgag
    31261 catctgctac gagctgggtt cctccactgg cgcgctgctc agcaagctcg cgacgcgcca
    31321 ttcggccact aaagcgcgct tcgtcggcat cgaaatcgtg cccgaaatga tcgcccaggc
    31381 gcgtgccgaa tccggctccc tgcccaatct cgagttcgcc caggaggaca tcaacctcta
    31441 ccctttcgag aaggccgatc tcatcgtgtc cttctgcacg atccagttcg ttccgcctta
    31501 cctgcggcaa accctgttcg atcggctcta ccagtgcctc aactggggcg gtgcgctgct
    31561 gctttacgag aaggtgcggg cgccggatgc gcgcttccag gactacatga catcggtgta
    31621 caccgacttc aagctggccc gcggctactc cccggatgag atcatcgcca aggcgcgcag
    31681 cctgaaacgt gcgcaggagc cattttcgac tcagggctac ctggacctgt tcaagcgcgc
    31741 cggctttgtc gacgtgatga gcgtcatcaa atgggtctgc ttcgagggat tcctagcgat
    31801 caagtaatga gcccgcgcgg tcgatcgctg atgtagccgc gctgcttgag cgtgcgcaca
    31861 gcgagataga ccgcactcca tgcgatcgcg ccgcaaccca gcgcggcggc cgatggcaaa
    31921 gcgcccgcgc ccgcgatgct gtactcgccc cagaagtgca ggccgaccag cgtcgccgcg
    31981 acgaggagga tcgtgccgta ctccttgacc agtacgcgct gccagttgaa gcgcagcgcg
    32041 ggctgcgtgc ggcgcaagct cgccagtcga ggccaccagc gattgacgcg ccgacagtag
    32101 tcctcgtagg cggcgccgaa cttgccgcgc agataggcct cctcggcgag cacgatggag
    32161 tggtatgcca ggacgatggc ggagatcacg atgagataaa acgcatgggc gtgaactacc
    32221 agcgacaggc cgatggcgat caggatattg ccggcgtaca agggattgcg gcagagggca
    32281 aacatcccgc cccgaaccag gctatcggca tagacgcgcc ggctcctccc gccgcgcacg
    32341 atgtactcgt agccgatggc gagtatccgc aggctctcgc cggcaagcgc cacgatgagg
    32401 ccggcgatct cgaatgcgat tctcaggtcg gtgctgccgt aggggagatg cggccggctg
    32461 aagatcagcg cgacgagaaa gacgaccgga ccgacgctat tccggtgccg gaagagggcg
    32521 ttgccgagct tgatcacacc aatgcagcgc tgcgcacgag ggtcgaatcg cggcacttta
    32581 cgcagtggct cgttaaggct gcgttaaggc cgactgcgtg cacgcggcat acaatcggcg
    32641 agtggccgaa agcccttccc acacgccagt gctgcgcgtt ctcatcgtcg aggacagccc
    32701 ggacatcctg gcgaacctgt acggctacct cgagccgctc ggctacagcc tggactgcgc
    32761 gcgagacggc ttcgctggcc tggaactcgc ggcgcatggc gcgcacgacg cgatcgtact
    32821 tgacctgatg ctgccgggac tcgacggcct tgacctttgc cgccgcctgc gccgagagct
    32881 gcgccgcagc acgcccgttc tgatcctgac tgcgcgcgat accgtcgagg acaaggtcct
    32941 cgggctgcgc tcgggcgccg acgactatct ggtcaagccg ttctcgctca acgagctcga
    33001 cgcacgcctg caggcgctgg tccggcgcag ccgcggtctc gtcagcgcga cgcttctgcg
    33061 cgtcggaacc ctacggttcg atccggccac ctacgaggcc acccgcgagg ggcggcgcct
    33121 ggagctctcg ccgatgggct atcgtatcct cggcgtgctg atgcgcgcga gtcccgcgct
    33181 ggtcacgcgc gacacgctcg aacgggagct gtggggcgac gagcggccct ccagcgactc
    33241 gctgcgcacg cacatccatg ccttacgcca ggctctcgaa aagccgttcg cgcagccggt
    33301 gctggtcacg atcccgggcc tgggataccg cctgattgat cccgatgcgg cctgaaagca
    33361 ccctgcgata ccggttgatg ctggcgttcg tactccttgc cgtggtgatc ggcggtgcat
    33421 ttgccggcgc agcgtatctc atcatcgagg cgacggagta tcagctgatc gagctgcgcc
    33481 ttgcgcacag tgccgctcgc tttttcgagc acgatccgcc gcaaagcgcg aatccgttcg
    33541 ccggcgtgac cgctgccgtc ggcacgcaga tacccggaga gctgggcgga ctggcgccgg
    33601 gtcaccacga gctccatctt ccggggcgtg acgtccatgt cctgatcgga taccacgccg
    33661 gcgagcgcta cgcgatcatc gaggacacga gcgatttcga gcggctcgag cagctcgcct
    33721 tcgtcggctt gtgggtcgcg ttcctctccg gcgttctgct tgcgctcgcg atggcgcgcg
    33781 cggtggtaaa ccgcgtcatc gcgccgctca catcgcttgc gagcgcggtg cagcgcgacg
    33841 acttgaggga gcagccgcag ctgctcaatg cggccgacga aatcggcgtt ctggcccggg
    33901 cattcgatgc cagggcatcg cagttgaggg aggttctcct gcgcgagcgc ctgtttaccg
    33961 ccgatgtaag ccatgagctg cgcacgccgc tcacggtcat gctgggagcc gccgagctcc
    34021 tgagcgcgag gctcgccgcg caagcggagc tgcgtgccgc agcggagcgc atccgccgca
    34081 ccgccgccga gacctccgtg cgcgtgagcg cattgctcca gctggcacgc tcgccggaaa
    34141 cgatcgaaag cacgctgctc tcgctgcggg atctggtgaa gcaggaaatc gagcgctgcc
    34201 ggccgctgct cgacggcaag ccggtgagcc tcaacctcga atcgagcggc aatgtctgcg
    34261 tgcactcggc tcacgatctc gccgccatcg ccgtcggaaa tctgctgcgc aacgcgtgca
    34321 ccttcacgca gcgcggcgcg gttcgagtcg tcctggagcc gaccggactg gtcattgaag
    34381 acacggggcc cggcgtcccg ccggaagtgc gcgaccgcct cctagcgcgc ttcgtgcggg
    34441 gtcgcgccga gccggcgacg ggatccggcc acggcctctc catcgtgaag cgcgtagccg
    34501 agcacttggg ctgggcggtc cggctggagg acggtcctca aggcggcagc cggttcacac
    34561 tgatattcgt ctcggaggca ccgcgccgaa cccgcgcaat gtcggcgatc agcgacaggg
    34621 cgtaggtaga gctcgactcc agaatcgccg cgccccggca gcaaggctgc gcgagctgtg
    34681 aaaaaatccg tggcgcgaat gtagggggag aagtgcgaga actagatgtc gcagaccgcg
    34741 acagacgatg cgctgagcta ttgcgcgcgc attcgcgatt tgatcctgca ccatggcggc
    34801 ggctgggctg acggcgacgc gctgagaaaa ttcaggctgc tgtccgacgc cgcgcgccgc
    34861 gccgcggacg acgcggaagc cacggagctg atgcgcaccg ccgcagagta cgcagcggat
    34921 ctgttttccg ggtctgacca tcagaaatgg gcgacgggca ggacctccgg cgcggacatc
    34981 ctgcggctgt gcatcctggc gaagctcgac gcattccgcg atcgagtggt gcacctccag
    35041 gaggcggcgc gagggcgaga cccctcggac cggatgcact acccctggga cgagtagccg
    35101 ccgtccaccg gaatcgccgc gccggtgatg aagtcggagg ccttgctgca caggaacacc
    35161 gcaattcccg agaggtcctg cggctcgccc cagcggccgg cgggcgtgcg gcgcagcacg
    35221 ttctcctgca gcccggggat gtccttgcgc gcgcgctggg taagggcggt gtcgatccag
    35281 ccggggagca ccgcgttcac ctggatgttg tcccgcgccc aggcgcaggc gagcgcgcgc
    35341 gtgagctgca cgatgccgcc cttgctcgcg ccgtagggcg cgacgaacgc ggcgccgaag
    35401 ag
//
LOCUS       DATBSZ010000059         6895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2124865, whole genome shotgun sequence.
ACCESSION   DATBSZ010000059 DATBSZ010000000
VERSION     DATBSZ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124865"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VIG70_03870"
     CDS             complement(<1..297)
                     /locus_tag="VIG70_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8249719.1"
                     /translation="MEVASLRSRLERLGSTVTSQVWRLGYASRFLAYLVLHSGTALRR
                     FRLTIAEVYFAGVLSLVIILVSGLFVGMVLALQGYETLQRFGASESLGVLVALSL"
     gene            complement(297..1106)
                     /locus_tag="VIG70_03875"
     CDS             complement(297..1106)
                     /locus_tag="VIG70_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249720.1"
                     /translation="MPQAPIVEIKNVSFGYDPARPVLRNIDLAIPQGAVVGIMGQSGC
                     GKTTLLRIIMGAYLPSSGEARVLGQATRGLDAAGLYQMRRKMGMLFQFGALFTDMSVY
                     ENVAFPLREHTDLPADLMHDLVMLKLQAVGLRGAAHLMPTELSGGMARRVALARSIAL
                     DPSLILYDEPFTGLDPISFGVIVRLIRSLNDSLGATSVIVTHDVQEALDVVDYVYFMA
                     AGRVIAQGAPDEIRASPEPFVNQFVNGKPDGPVPFHYPAPEYGADLEVASA"
     gene            1125..1871
                     /locus_tag="VIG70_03880"
     CDS             1125..1871
                     /locus_tag="VIG70_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249721.1"
                     /translation="MREAAPALHVAALAKRYGEFEALSGVDLDVAPGEAFGLVGANGA
                     GKTTLIKCMLDLTACDSGTVEIFGVAAGNPAARRRLAYLPERFNPPHYLRAREFLAMM
                     LELAGARYDPSHVLGILDQLELDRGALEQPVRSLSKGMTQKLGLAGCFVVPRDLYVLD
                     EPMSGLDPAARVAVKSALRRLAAEGRTLFFTSHVLADVEELCTAIAVLERGRVRWRGT
                     PAALCARYADANLERAFMKCIRDEHAAAQA"
     gene            1846..3216
                     /locus_tag="VIG70_03885"
     CDS             1846..3216
                     /locus_tag="VIG70_03885"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8249722.1"
                     /translation="MSTPQPKPRDKPGLLIVDDDPLITDTLAFALGSDFSVYACDSRP
                     HAIELLRQLEAPPPLALVDLGLPPAPHAPDEGFRLIADLLAHSPRMKIFVLSGQNDAA
                     NARHARALGAWEFVAKPADPASLARLLARGLEVPAQEDADFVGASPALAKLKSQIAQF
                     AAAPYPVLIEGESGSGKELVALRLHRLSPRAARPYFSLNCAAISPQLVEPTLFGYVKG
                     AFTGAQSSKAGFFEDAADGTLFLDEIGELPLEMQAKLLRVLENGEYQRVGETQRRTAR
                     CRVIAATNRDLRRAVREGAFRADLYHRLSVFTLGVPPLRELDQDKLLLLDHFRKKSFD
                     FDDSATARWLRYAFPGNVRELRNIVIRLSAKYPGQRISAAELEPELDLDAEIKEKSGL
                     VEQALRQLERTGQFDLDETLKAWERGYIEAALRLTQGNVTQAAKLLGINRTTLYSRMS
                     GQDEEK"
     gene            3219..4661
                     /locus_tag="VIG70_03890"
     CDS             3219..4661
                     /locus_tag="VIG70_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8249723.1"
                     /translation="MALYLEHFGLTEPPFRITPHTDFFFEGADRGATLEALIYAVLHD
                     EGIVKVSGEVGSGKTMLCRVLMERLPQHVVTIYLATPSLASDEILHAIGDELELKLSP
                     ERRSVALRELQEHLISLYGAGRRVVVLIDEAHVMPDDTLEQVRLLSNLESNRHKLLQI
                     VLFGQPELDATLAKAALRQLRDRITHAFRMRPLVVTEVAKYLSFRMRAAGYRGPEVFT
                     PRAVRLVARASGGLSRRINILADKALLSAYTENTHAITDRQVRAAIADSEFAAARKPR
                     LALYLAAAAAAGVAIGAAAQWIATLPLGTRSVAPPPAPAPVVRAPAPALPAAPAAPVT
                     EKEPEPPAQPAPEPEAEAEPQLTAEQARRLAGYSAAGQRLLAERLAATREILERADEE
                     HYALELFVTENTDPARMERFLLRARELVPLEELYVIPVAAGSQYRLWVIFGEFGSRDE
                     AAAAVRRLPPRYQEAFRAAPRSFGELRRQL"
     gene            4750..6489
                     /gene="mshL"
                     /locus_tag="VIG70_03895"
     CDS             4750..6489
                     /gene="mshL"
                     /locus_tag="VIG70_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HEY8249724.1"
                     /translation="METGKAARLTAILVLLGGCGQAPVKPAATHIRADEPRAEGTIPP
                     PVQVTPVLPQPKVTARPETYSVVVNNVRAQELLFALARDARLNVDIHSDITGTVTLNA
                     IDQTLPQLLTRIARQVDMRYEIAGQNLFVMRDTPFLRIYRVDYVNMARDARSVAQLST
                     QVSGTQTGVTTTAQNNSTATINSVSVNKFWDTLVANIKEILQETDKVLPAGASAAPPV
                     AAPAQPGAPAAATPVAAVPSATFREAASVIANPETGILNIRATSRQHEKVQEFLDQVM
                     TNAKRQVLIEATVAEVQLNNEYQRGIDWQRLRTNAATVGVPSFGVGNSGVEFGQSSGN
                     TPAGISTNAFIFGGAITSLNFNFALRLLESFGDVRVLSSPKIAVLNNQTAVLKVVDNL
                     VYFTIKADTTQNQNSSLTTFTTTLNSVPVGFILSLVPQISDSDSVVLNLRPTISRKIG
                     DVPDPNPSLAASNPPVVSLIPVIQTREMESVLRVQSGQVAVLGGLMQDSRSSVEDGIP
                     GVNAIPWLGSLLGQRKDLNQKTELVIFLRTVVVRDASIDGDYAAFRSLLPGPDFLSKP
                     NPSRPPRPEDRSE"
     gene            6491..>6895
                     /locus_tag="VIG70_03900"
     CDS             6491..>6895
                     /locus_tag="VIG70_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249725.1"
                     /translation="MSLLLEALKKAEKAKEEAQRRAQGESPEAAAAPPPREPEPAPEP
                     AADIRRPLEIIGDERPPKHVEPPAFALEPEKPRPEPAAAPRAQPKAEPQASERAAARK
                     VFEAKFKESNPRLPFFITMGALGAFAVGTVVYF"
BASE COUNT         1134 a         2468 c         2301 g          992 t
ORIGIN      
        1 cagcgacagc gccaccagca cgccgagcga ttccgaggcg ccgaagcgct gcagcgtctc
       61 gtagccctgc agcgcgagca ccatgccgac gaacagcccc gacaccagga tgatgacgag
      121 cgacagcacg ccggcgaagt acacctcggc gatcgtcagc cggaagcgcc gcagcgcggt
      181 gcccgagtgc agcacgagat acgcgaggaa gcggctggcg tagccgaggc gccagacctg
      241 cgaagtgacg gtgctgccca ggcgctccag gcggctgcga agcgaggcga cttccatcag
      301 gccgaagcca cctctagatc ggcgccgtat tccggcgccg ggtagtggaa cggcaccggg
      361 ccgtcgggct tgccgttcac gaactggttg acgaacggct cggggctcgc gcggatctcg
      421 tcgggcgcgc cttgcgcgat gacgcggccg gcggccatga agtacacgta gtcgaccacg
      481 tcgagcgcct cctgcacgtc gtgcgtcaca atgaccgacg tcgcgccgag cgagtcgttc
      541 aggctgcgga tcaggcgcac gatcacgccg aaagagatcg ggtcgaggcc ggtgaagggc
      601 tcgtcgtaaa gaatgaggct cgggtcgagc gcgatcgagc gcgccagcgc cacgcgccgc
      661 gccatgccgc ccgagagctc ggtcggcatg aggtgcgcgg cgccgcgcaa tcccaccgcc
      721 tggagcttga gcatcaccag gtcgtgcatc aggtccgcgg gcaggtcagt gtgctcgcgc
      781 agtgggaagg cgacgttctc gtacaccgac atgtcggtga acagcgcccc gaactggaac
      841 agcatgccca tcttgcggcg catctggtag aggcccgcgg catcgagccc gcgcgtggct
      901 tggccgagca cgcgcgcctc gccgctcgac ggcaggtagg cgcccatgat gatccgcagc
      961 agcgtggtct tgccgcagcc cgactgcccc atgatgccga cgactgcgcc ttgcgggatc
     1021 gcgaggtcga tgttgcgcag caccggccgc gccgggtcgt acccgaacga gacgtttttt
     1081 atttcaacaa taggggcttg cggcacggcg taatattatc aagcatgagg gaggcggcac
     1141 cggcactgca tgtcgcggcg ctcgcgaaac gctacggcga attcgaggcg ctgtcgggcg
     1201 tcgacctcga cgtcgccccg ggcgaggcgt tcggcctggt cggcgcgaac ggcgccggca
     1261 agaccacgct catcaagtgc atgctggatt tgaccgcatg cgactccggc acggtggaga
     1321 tcttcggcgt cgccgcaggc aacccggcgg caaggcgccg gctggcttac cttcccgagc
     1381 gcttcaaccc gccgcattac ctgcgcgcgc gcgaattcct cgccatgatg ctcgaactcg
     1441 ccggcgcgcg ctatgaccca tcccatgtgc tggggatcct cgatcagctc gagctcgatc
     1501 gcggcgcgct cgagcagccg gtgcgcagcc tttcgaaggg catgacgcag aagctcgggc
     1561 tcgccggctg cttcgtcgtg ccgcgcgatc tctacgtgct cgacgagccg atgagcggcc
     1621 ttgaccccgc cgcgcgggtc gcggtgaaat cggcgctgcg gcgcctcgcc gccgagggca
     1681 ggacgctgtt cttcacctcg cacgtgctcg ccgacgtcga ggagctgtgc accgcgatcg
     1741 cagtgctcga gcgcggcagg gtgcgctggc ggggcacccc cgcggcgctg tgtgcgcgct
     1801 acgccgatgc gaacctggag cgggccttca tgaaatgcat ccgcgatgag cacgccgcag
     1861 cccaagccta gggacaagcc cggcctgctg atcgtcgacg acgatccgct gatcacggac
     1921 acgctggcgt tcgcgctggg cagcgacttc agcgtctacg cctgcgactc gcgcccgcac
     1981 gccatcgagc tgctgcgcca gctcgaagcg ccgccgccgc tcgcgctggt cgatctcggg
     2041 ctgccgccgg cgccgcacgc gcccgacgag ggcttccgcc tgatcgcgga cctgctcgcg
     2101 cattcgccaa gaatgaagat tttcgtgctc tccgggcaga acgacgccgc caacgcgcgc
     2161 cacgcgcgcg ccctcggcgc ctgggagttc gtcgccaagc cggccgatcc ggcgagcctc
     2221 gcgcgcctgc ttgcccgggg cctcgaggtg ccggcgcagg aggacgccga cttcgtcggc
     2281 gcgagccccg ccctcgccaa actgaagagc cagatcgccc agttcgccgc cgcgccctac
     2341 ccggtgctga tcgaaggcga gtccggcagc ggcaaggagc tggtggcgct gcgcctgcac
     2401 cggctctcgc cgcgcgccgc gcgcccctac ttcagcctga actgcgccgc gatctcgccc
     2461 cagctcgtcg agccgaccct gttcggctac gtgaagggcg ccttcaccgg cgcccagtcg
     2521 agcaaggccg ggttcttcga ggacgcggcc gacggaacgc tgttcctcga cgagatcggc
     2581 gagcttcccc tcgagatgca ggcgaagctg ctgcgcgtgc tggagaacgg cgagtaccag
     2641 cgcgtcggcg aaacccagcg ccgcaccgcg cgctgccgcg tgatcgccgc caccaaccgc
     2701 gacctgcggc gcgcggtgcg cgagggcgcg ttccgcgccg acctttacca tcgcctgtcg
     2761 gtgttcactc tcggcgtgcc gccgctgcgc gagctcgacc aggacaagct cctgctgctg
     2821 gatcacttca gaaaaaaatc tttcgacttc gacgacagcg ccacggcgcg ctggctgcgc
     2881 tacgcctttc ccggcaacgt gcgcgagctg cgcaacatcg tgatccggct ctcggccaag
     2941 taccccggcc agcgcatctc ggccgccgag ctcgaaccgg agctggacct cgatgccgaa
     3001 atcaaggaaa agagcgggct cgtcgagcag gcgctgcggc agctcgagcg cacaggccag
     3061 ttcgacctgg acgagacgct caaggcgtgg gagcgcggct acatcgaggc cgcgctgcgc
     3121 ctgacgcagg gcaacgtcac gcaggcggcg aagctgctcg gaatcaaccg caccaccctg
     3181 tattcccgca tgtcgggaca ggacgaggag aagtagcgat ggcgctctac ctcgagcact
     3241 tcggcctgac cgagcccccg ttccgcatca cgccgcacac cgacttcttc ttcgagggcg
     3301 ccgaccgcgg cgcgacgctg gaggctctca tctacgccgt gctgcacgac gaaggcatcg
     3361 tcaaggtgtc gggcgaggtc ggcagcggca agaccatgct gtgccgcgtg ctgatggaac
     3421 ggctgccgca gcacgtggtg accatttacc tcgccacgcc ttcgcttgcg agcgacgaga
     3481 tcctgcacgc gatcggcgac gagctcgagc tcaagctttc gccggagcgc cgcagcgtcg
     3541 cgctgcgcga gctgcaggag cacctgatct cgctctatgg cgccgggcgc cgcgtggtgg
     3601 tcctgatcga cgaagcgcac gtcatgcccg acgacaccct cgagcaggtg cggctgcttt
     3661 cgaacctcga atcgaaccgc cacaagctgc tgcagatcgt gctgttcggc cagcccgagc
     3721 tcgacgcgac gctcgccaag gcggcgctgc gccagttgcg cgaccgcatc actcacgcgt
     3781 ttcgcatgcg cccgctcgtg gtgacggagg tggcgaaata cctgtcgttc cgcatgcgag
     3841 cggccgggta tcgcgggccg gaggtgttca cgccgcgcgc ggtgcgcctg gtcgcccgcg
     3901 cttcgggcgg gctctcgcga cgcatcaaca tcctcgccga caaggcgctg ctctccgcct
     3961 acacggagaa cacgcatgcc atcaccgacc gtcaggtgcg cgcggcgatc gcggattcgg
     4021 agttcgccgc ggcgcgcaag ccgcggctcg cgctgtacct cgccgccgcc gcggccgccg
     4081 gcgtggcgat cggcgcggcg gcgcagtgga tcgcgaccct gccgctggga acgaggagcg
     4141 tcgcgccgcc gcccgccccc gcgccggtgg tccgggcgcc cgctccggcc ctgccggcgg
     4201 cgccagcggc gccggtcacg gaaaaggagc ccgagccccc ggcccaaccc gcgcccgagc
     4261 cagaagcgga agccgagccc cagctcaccg cggagcaggc gcgcaggctc gccggctatt
     4321 cggccgcggg ccagcggctg ctcgcggagc gcctggcggc gacgcgcgag attctcgagc
     4381 gcgccgacga ggagcactac gcgctcgagc tcttcgtcac cgaaaacacc gatccggcgc
     4441 gcatggagcg ttttctgctg cgtgcgcgcg agctggtgcc gctcgaggag ctctacgtga
     4501 ttccggtcgc cgccggcagc cagtaccgcc tgtgggtgat cttcggcgaa ttcggcagcc
     4561 gcgacgaagc ggccgcggcc gtgcggcggc tgccgccgcg ctaccaggaa gccttccgcg
     4621 ccgctccgcg cagtttcggg gagcttcgcc ggcagttata aattgtagaa agtgctagcg
     4681 cgacagtcgc ttacgctact ttcctcaagg cacttctgtg ctattcttgc cgtcggaggg
     4741 agcacccgca tggaaacggg taaggcggct cgactaactg cgattttggt cctgctcggc
     4801 ggctgcggcc aggcgccggt caagccggcg gcgacgcaca tccgcgccga cgagccgcgc
     4861 gccgagggca cgatcccgcc gccggtgcag gtgacgccgg tgctgccgca gccgaaggtg
     4921 accgcgcggc ccgaaaccta cagcgtggtg gtcaacaacg tgcgcgcgca ggagctcctg
     4981 ttcgcgttgg cgcgcgacgc gcgcctgaac gtcgacatcc attccgacat caccggcacc
     5041 gtcacgctga atgccatcga ccagaccctg ccacagcttc tcacgcgcat cgcgcgccag
     5101 gtcgacatgc gctacgagat cgccggccag aacctgttcg tgatgcgcga tacgccgttc
     5161 ctgcgcatct accgcgtcga ttacgtgaac atggcgcgcg acgcgcgcag cgtggctcag
     5221 ctttccaccc aggtgagcgg cacccagacc ggggtgacga ccacggcgca gaacaactcc
     5281 actgccacca tcaactcggt gtcggtgaac aagttttggg acacgctggt cgccaacatc
     5341 aaggagatcc tgcaggagac cgacaaggtg ctgccggcgg gcgcgtctgc cgcaccaccg
     5401 gttgccgcgc cggcgcaacc cggcgcgcct gcggcggcca cgccggtggc ggcggttccc
     5461 tcggcaacgt tccgcgaggc cgcctcggtg atcgccaacc ccgagaccgg catcctcaac
     5521 atccgcgcca cctcgcgcca gcacgagaag gtgcaggagt tcctcgacca ggtgatgacc
     5581 aatgcaaagc gccaggtgct gatcgaggcg acggtcgccg aggtgcagct gaacaacgag
     5641 tatcagcgcg gcatcgactg gcagcggctg cgtaccaacg cggccacggt cggcgtgccg
     5701 agcttcggcg tgggcaacag cggcgtggag ttcggtcaga gctcgggcaa cacgcccgcc
     5761 ggcatcagca ccaacgcctt catcttcggc ggcgcaatca ccagcctcaa cttcaacttc
     5821 gcgctgcggc tgctcgagtc cttcggcgac gtgcgcgtgc tctccagccc gaagatcgcc
     5881 gtgctcaaca accagaccgc ggtgctgaaa gtggtcgaca acctggtgta cttcaccatc
     5941 aaggccgaca ccacccagaa ccagaactcc tcgctcacca ccttcaccac cacgctcaac
     6001 tcggtgccgg tcggtttcat cctgagtctg gtgccgcaga tctcggactc cgactcggtg
     6061 gtgctcaacc tgcggccgac gatctcgcgc aagatcggcg atgtccccga ccccaacccg
     6121 agcttggccg cctccaatcc tcccgtcgtc agcctgatcc cggtgatcca gacgcgcgag
     6181 atggaatcgg tgctgcgcgt gcagagcggc caggtggcgg tgctcggcgg gctgatgcag
     6241 gattcgcgca gcagcgtcga ggacggcatt cccggagtca acgccatccc gtggctcggc
     6301 tcgcttctcg gccagcgcaa ggacctcaac cagaagaccg agctggtgat cttcctgcgc
     6361 accgtggtcg tgcgcgacgc cagcatcgac ggcgactacg cggcgttccg cagcctgctg
     6421 ccggggccgg atttcctctc caagcccaac ccgagccggc cgccgcggcc ggaagaccgc
     6481 tccgaatgac atgagcctgc tgctcgaggc gctcaagaaa gcggagaagg cgaaggaaga
     6541 ggcgcagcgc cgggcccagg gcgaaagccc ggaggcggcc gccgcgccgc cgccgcgcga
     6601 gcctgaaccc gcgcccgagc cggccgccga catccgccgg ccgctcgaga tcatcggcga
     6661 cgagcgccct ccaaagcacg tcgagccgcc ggcgttcgcc ctcgagcctg agaagccccg
     6721 gcccgagccc gcggcggcgc cgcgagcgca gccaaaagcc gaaccccagg cgagcgagcg
     6781 ggcggcagcc cgcaaggtat tcgaggcgaa gttcaaggag tccaacccgc gcctgccgtt
     6841 cttcatcacc atgggcgcgc tcggcgcgtt cgccgtcggc accgtggtct acttc
//
LOCUS       DATBSZ010000060        31447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1168753, whole genome shotgun sequence.
ACCESSION   DATBSZ010000060 DATBSZ010000000
VERSION     DATBSZ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 31447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31447
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168753"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VIG70_03905"
     CDS             <1..384
                     /locus_tag="VIG70_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HEY8249726.1"
                     /translation="ERVLQVPSQSAAILTVLMLRGPQTAGELRINAERLHSFSDISAV
                     QGFLDELAARPAGPLVVELPRLPGARENRWAHLLSGAPNIETSKVETAKRPATSLEAD
                     VARLEAELAALKDLVARIAKQVGIS"
     gene            complement(639..1091)
                     /locus_tag="VIG70_03910"
     CDS             complement(639..1091)
                     /locus_tag="VIG70_03910"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8249727.1"
                     /translation="MRLVVLLAVLLAGCATPPGPAPEAEPKPEAPPPVAAPAPKENIA
                     IAGLMDSARTEAAAGNLAGAAASLERALRIEPRNPRLWHELARVRLKQGQYAQAESVA
                     ARSNSWVGEDKALRAENWRLIAESRRARGDADGAQAALEQAGASREHK"
     gene            complement(1088..3400)
                     /gene="mrcB"
                     /locus_tag="VIG70_03915"
     CDS             complement(1088..3400)
                     /gene="mrcB"
                     /locus_tag="VIG70_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HEY8249728.1"
                     /translation="MLRRRPKLWKVVRLALLATVLAVAGFTLYLDFRVRSEFEGRRFA
                     LPARIYARPLELHAGLRIAQADVEDELRSLGYREAPPEGEAGWFAKKDDSLEIALRPF
                     VFWDGKQPAKRVTVVFDQNRIAALRDTGGAELALARLEPLAIGGIYPAGNEDRVLVRL
                     GDVPKHFVQALIAVEDRSYFSHAGFDLRGLARAARSVFSDKVQGGSTITQQLVKNFFL
                     TPERTLQRKFTELLMAVLLEIHYDKNEILETYLNEIYFGQDRDRAIHGVGLAAQFYFG
                     KEVQYLTAAESALLVGMVRGPAIYDPYRHPQRALERRNLVLRETRDQKGITPDEYADA
                     RSSPLNVSKKTGTGTTPYPAFLQLVHRQLRRDYDEADLRSEGLRIFTTLDPRVQDAAE
                     TALAKRLAQYDKEKRFGQQAGLEGAVVVTDPQSGEVQALVGGRDPRYRGYNRALDAAR
                     PVGSLLKPAIYLTALSEPARYTLLTPIDDGPFVWKSRGAPDWEPANYDKKFHGIVPLR
                     TALAQSYNAAAARLGTDLGIERVLDNVRRLGVDRPLRPFASTLLGAVELSPFEVAQMY
                     QTIASGGFRSPLRAIRDVTTQEGRPLSRYQLAVEQVFAPEPIYLLAAAMQDVVREGTA
                     QGLNRFLPPETAAAGKTGTTDEQRDAWFAGFTGDRLGIVWLGYDDNRAARLSGAAAAL
                     PVWGDLMSVLAPEPLVLARPERIEMVSIDPQTGLRGGTSCPGATEVPFVQGSAPSERA
                     PCSGAMDAAVEVVGKSVERAKSWLDRLFGR"
     gene            3444..3842
                     /locus_tag="VIG70_03920"
     CDS             3444..3842
                     /locus_tag="VIG70_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8249729.1"
                     /translation="MLTIRGIDHVVLRVRDIDRVRKFYCDVLGAHHVAYRPELGMSHL
                     RIGAAMIDLVKGEGPTGCNMDHFCLRVEPFDQDAIVAHLKKHGVAVGDIRNRFGAEGN
                     GISIYLTDPEGNTVELKGPSDGTAQPPTPK"
     gene            complement(3821..4516)
                     /locus_tag="VIG70_03925"
     CDS             complement(3821..4516)
                     /locus_tag="VIG70_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249730.1"
                     /translation="MIELKKINTFYGLGHILHDLSLSVAEGEVVALLGRNGAGKTTTL
                     RSITGLTPPKSGEIRYKGRDIAGLDAHRVSRLGIALVPETRDIFSYLTARENLAIARR
                     PQSRWQMDNVLGRFPSLKERLNNKGRQLSGGEQQMLAIARALLTGPDLLLLDEPSQGL
                     APLVVSAVMSTIQELKKERVSMLLVEQNAEMALQLADRVYVIDHGTVVFEGTPAALRA
                     DRQVTTTYLGVGG"
     gene            complement(4513..5283)
                     /locus_tag="VIG70_03930"
     CDS             complement(4513..5283)
                     /locus_tag="VIG70_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249731.1"
                     /translation="MEPVLGMEGILETRQLSKSFGALAAVKDVSLSIAAGSLHSVIGP
                     NGAGKTTLFNLLTGTFPPTSGEILFDRKNITGTRADRIAHLGLARSFQRTNVFPAFSL
                     LDNVWVAAFATGRSWNGLLFRKTDRYPEVMERARAALSDVGLLSKANQLAREISHGEQ
                     RQLELAIALAAAPRVLLLDEPAAGLSPEETRNMVALVRTLKGRYTIVLIEHKMDIIMS
                     VSDRISVMHFGSLIAEGAPEEIQRNAEVRRAYLGGIAA"
     gene            complement(5235..6164)
                     /locus_tag="VIG70_03935"
     CDS             complement(5235..6164)
                     /locus_tag="VIG70_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8249732.1"
                     /translation="MKYQNILLVAAVIALPFIVRPVIASEIWIFAIFGLGLNLLLGYT
                     GLLSFGQSTFFGSAAYVAGWLLKHYGINVFFALGIGVGVGALSALLVGYLCVQRSGLY
                     FIMLTFALNQLFYFVAYQWTTVTGGEDGMHGVPRPDLLGINFKDPMTYYVFISLLFLA
                     SLWAMRRIVESPLGRILQAIRENEVRAEAIGYNVPRFKLLAFVIGGAFSGLAGVLYGM
                     LFGIVPLEAISFVFSGNVVFATLIGGSGSLYGPIIGSFVFIWLSESVSVIWARWPLLL
                     GVAFVIVVLFFRGGVVEAWNRFWAWKASSKPAS"
     gene            complement(6161..7018)
                     /locus_tag="VIG70_03940"
     CDS             complement(6161..7018)
                     /locus_tag="VIG70_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8249733.1"
                     /translation="MNLLLSQILNGIATGMLYALMGLGLSLITGVLNIPNFAHGALFA
                     VGAYLLVSTVNVIGNFWLALLIAPIGVAILGLVIEYAGIRRLYSAGHDYQLLFTFGLA
                     LILSEAIIIVWGPVGYSHLPPPILRGGLDLGFTFYPKYRLFVMVVAGLLVAATWLFLE
                     KTRYGAIMRAGIENKEMVSLLGIDIHKLFTVAFALGAYLAGIAGALTAPIRGLSPQMG
                     VDMLGIAFVVVALGGLGNLLGAIAAGLLIGVAQAVVAAYWTEASTAVIFAVMAAVLLV
                     RPQGLFGIR"
     gene            complement(7015..8229)
                     /locus_tag="VIG70_03945"
     CDS             complement(7015..8229)
                     /locus_tag="VIG70_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8249734.1"
                     /translation="MSNSKRRKILKLGAATAALGFPAIVRAQPKEIVMLGIWPATGAF
                     ADVGPILDRGMKMALDERNMRILGRPIRYIVRDSATQAGAATRRVEEAVDGEGAKFVI
                     GPWSSGVALAVSEVAKRKKVFHIFSGGTEDISGARCHRYSFQWAASPYTAAKTVVDNF
                     MKANPKAKRWHLLVADYAFGWSVEKYIKEVGKTHGIEFVGADRHPLGEREFSNYVQKA
                     AANRPDAVAMINFGLDAVSGAREIFNFGLTPKVPLIMTWSSGVEELVQLAPEIRENIW
                     VGSNFYYTADTPVAKEFVKSYQGKFGSPPGYAPSAAYSMTRLTLAAMDKSKIGEVADV
                     VKAMEGIEVDDLLGRMRVDPATHQTIRPYFFMRCKPKSAMKHPMDFADIIATGDAPLP
                     KEYAACKDIGSF"
     gene            8285..8677
                     /locus_tag="VIG70_03950"
     CDS             8285..8677
                     /locus_tag="VIG70_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249735.1"
                     /translation="MRRWIAIAVLQLAAGAAQACLFATSTPPEGWYQWASGLFAGDVT
                     GVAKDGEKPIDVVTVRVVETFKGPDASHGTLTVRLSNRYWTNCRVARPAAGARVLVAM
                     NANSDAMLVPLSESYAEQLRRQRAIGVR"
     gene            8681..8920
                     /locus_tag="VIG70_03955"
     CDS             8681..8920
                     /locus_tag="VIG70_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249736.1"
                     /translation="MIKTATVFAIVLSLAAATAAAQNDSGKKTSDGFGNLLKGMGQEI
                     NKAGGSSTAPKKDAKKADAKKSDAKKKDAKAAKKE"
     gene            complement(8930..9706)
                     /locus_tag="VIG70_03960"
     CDS             complement(8930..9706)
                     /locus_tag="VIG70_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8249737.1"
                     /translation="MDLGLRDKKVLITGGSKGIGLACARAFVAEGARVALVSRSEENL
                     NKARDKLKGAYTVAADLTDAAAAAAMVEQVEKEFGPIDVLVNSAGAAKRTDADDLTPA
                     AWRAGMDAKYFSYINVIDPVIKRMGKRGRGAVINVIGSGGKVASPTHLAGGAANAALL
                     LATAGLAYAYASKGVRVVGVSPGVTKTERVAEGLKAEAKRANVSVEQAEKQMVARLPM
                     GRPAEPEEIADIVVFAASERGRYLTGANISTDGAASHVVI"
     gene            complement(9712..10668)
                     /locus_tag="VIG70_03965"
     CDS             complement(9712..10668)
                     /locus_tag="VIG70_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249738.1"
                     /translation="MLRIVFLALGLLWALPAAAQPYPSQPIRLIAPFPPGGSVDIMAR
                     LIAEPLAGQLGGKIVIDNRSGASGNIGMEAAARAKPDGYTVVLNTIPLATNQALFDKL
                     SWDPVKDFAPIGMVATAPHVLVVPTKVHASKVDDLIRLARTNPGKLTYASAGVGTTFH
                     LCAEMFKDSTSTFILHVPYRGGGPALLDTLGGQVDMSFPTLSAALPHVKAGTLRALGV
                     TSTTRSPLLPDVPTLQEAGVKDFQFTQWLVLLAPAGTPREVVAHLNEGLRKTLTSKDV
                     RDKFSQQGFDAFITTPEDAAKFIASEVKRFGTLIKNRKITAE"
     gene            10871..11896
                     /locus_tag="VIG70_03970"
     CDS             10871..11896
                     /locus_tag="VIG70_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY8249739.1"
                     /translation="MASAFAMPAFAQVPPELKIYGRVNVSMERIETDNSTNPAVPNQS
                     NYEFVDNSSRIGFAYNKDLTAGLKAIFQIESNVNVTDGGQTFSSRDSFAGLQGGFGTI
                     RLGRTIGPVYYATYDYISMHNHDTGTSSDALLAGTVVGNQGFMNNTLWYTSPKIGNFT
                     IEGAFSLLGEQRQAPGMDQPRHIGLVGAYDTGPLHAAISYANTKNDADLGGGRPSDAT
                     AYTFGGAYDFKFMVLGALWEMAERDIATGSVDSDYWRIAAMFPVGQHEFHVNYGLVDA
                     DGDRGAQQWTLGYNYNITKLTKVYAFYTMVDNDTAGNFTMNSTRTAGAATGAQYTSIA
                     VGLRHNF"
     gene            11993..12766
                     /locus_tag="VIG70_03975"
     CDS             11993..12766
                     /locus_tag="VIG70_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY8249740.1"
                     /translation="MKAPSFAYAKPRSLAEAFDLLERPGAKILAGGQSLIPSLNLRLS
                     APELLVDITGISGFSGIALANGSLRIGALCTHAQLEKSEDVRKHLPLVAEAVSHIAHP
                     AIRNRGTLGGSLALADPAAELPACALALDAVLVVAAKKGERRVKAADFFKGLFDTDLK
                     PAEILVGVEFPKADKSVFLELTRRQGDYAIVGLAAVTRASQKRISFFGIAGKPVLLTP
                     KSLDEAKASVPPPAADLYHSSSTKLHLAKVLLDRAWNRL"
     gene            12751..13233
                     /locus_tag="VIG70_03980"
     CDS             12751..13233
                     /locus_tag="VIG70_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8249741.1"
                     /translation="MEQTLTTTMTVNGTQVTRSVPPRQHLVDFLREELGLTGSHLGCE
                     HGVCGACTIRVNGEIVRGCLMLAVQANGCRVDTIEGLSDSKELEKLQGAFHRRNALQC
                     GFCTPGMLMAAQDLIRSGKKATREEIRAHISGNYCRCTGYHAIVDAIEEVIQAEVNHG
                     "
     gene            13226..15613
                     /locus_tag="VIG70_03985"
     CDS             13226..15613
                     /locus_tag="VIG70_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8249742.1"
                     /translation="MGKAELPLHAPGTQVDKGYIGKSVPRPNAQRLLQGRGTFTDDLR
                     FPRLAHVAFFRSPYAHASIKKLDFSKAIKSPGVIAVFDGPTVAEYCKPWVAVLAHLKG
                     IKSPPQHAIAIGRACWQGEPVAAVVAESRSQAEDAVALIEAEWEELPVVVDMEDSLKN
                     TKTIHPELGDNICFKREHDTGGVDEAFAKADVVVEDTFHFGRHTGVCLEGRAILADYN
                     AAEHSLTVYHATQAPHMMQDIFSRHLGIPEANVRVIAKDIGGAYGIKVHVYPDEMATA
                     ALSVMLRRPVKFLADRLESFLSDIHAREHRARVRLACTKSGEILAFELDDLTAIGPYS
                     VYPRTSGIEGNQVVNLTGGPYKHQKYKGRTNVVFTNKNVTCQYRAVGHPIAVALTEGI
                     VDLAAQKLGMDPAQFRKVNLIPDDAYPYTFPSGLKFEKLSHHKALDLLLKITEYDKLR
                     AEQSRLRNQKIYRGIGLAAMIEVTNPSPAFYGVGGARISAQDGATLRLEPTGMVTVLC
                     SVTEQGQGTEGIFAQIAASAVGVSIEKVKVITGDTGVTPYGGGTWASRGAGIGGEAVL
                     QAGRALRSNMLDVASKILNLDKAELDIRDNAVINKLSLERKLELAELGRIGYFRPDTL
                     PADYQSELTVTRHYTPRQYGFTITNGIQASLVEVDPETGFVKLLKHWCVEDSGTVINP
                     MLVDEQIRGGVVQGIGAALYEECLYGPDGQLLNGNMADYLVPMSGEMPDIVCAHIETP
                     TLQSQLGAKGVGEAGTAGAPAAVMNAINDALAPLNARVTRMPFTPERILAALGKV"
     gene            complement(15650..15982)
                     /locus_tag="VIG70_03990"
     CDS             complement(15650..15982)
                     /locus_tag="VIG70_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8249743.1"
                     /translation="MARKREKVDPGSGDVFVDLGFADAGERRLRVQLAIRINDLIAER
                     GLTQSKASELLGVSQPHVSELKNYKLSRFSSERLLRFLTRLDRDVEILIRPKAKGRQA
                     GHLTISMG"
     gene            complement(15966..16304)
                     /locus_tag="VIG70_03995"
     CDS             complement(15966..16304)
                     /locus_tag="VIG70_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006406736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY8249744.1"
                     /translation="MGSAKKDLLEFPDDVVDGLGYALGVVQAGITPASAKPWKGEGPG
                     VFELVQDRRGGTWRSVYTVRFRKAVYVLHCFQKKSPSGIRTAQTDVELVQRRLKAARE
                     DYEVRYGEKA"
     gene            16469..17533
                     /locus_tag="VIG70_04000"
     CDS             16469..17533
                     /locus_tag="VIG70_04000"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8249745.1"
                     /translation="MDNRVSSAISHWAPRFVSNGVLLADFEDVTGSLERWEDWCAAWS
                     ARARVHEDLGRASLKEGHKLSASEHLIRAAIYYHFAKFVFVQDPKQMRAAHMKAVACY
                     RDGIALARPPGERVEIAFSGKTIAGVLRKPGSPKPPVLIMAPGLDSTKEEIHAYEEPF
                     LARGIAVLAIDGPGQGEAEYDIPICGDYERAAKAVVDWIEKRADLNANQVAIWGVSLG
                     GYYAPRAAAYENRIRACIALSGPFEWERIWDGLPELTRETFRVRSHAANETEAREKAK
                     QLTMKEAAPRITCPIFIVTGRQDRLVPAAHAEQLAKAVSGPVELLIVEDGGHNANNRP
                     YRYRSRTADWLAMQFGLPKV"
     gene            17544..18485
                     /locus_tag="VIG70_04005"
     CDS             17544..18485
                     /locus_tag="VIG70_04005"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY8249746.1"
                     /translation="MRFLVAVLVLALGAVAQAQTFPSKTVRFISGVTPGSASDTMARI
                     LAEKLGASLGQPVIVENRLGAGGLVGAKWVATQEPDGHNIMMYASAFTVSTLLNTGLD
                     PKELVPVATVATIPTILITPPGKYKSEKDFVAAAKAQPGKLVCATAGVGSATHMALER
                     FRFAAGFEVLNVHTKGAGEALTEVISGRADCYFALVFQAQKMKDAGKVDAHLVSGPKR
                     TSLMPALPTTEEAGYPGSSYNFWVGTLVAAKTPRPIVERLAKEINALVQSPEISDRIT
                     KLGADPLTLSIAEFQAMIDRELRDNAELIKKAGIKAN"
     gene            18487..20412
                     /locus_tag="VIG70_04010"
     CDS             18487..20412
                     /locus_tag="VIG70_04010"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HEY8249747.1"
                     /translation="MKKDFFEEQDGVAVNPSSEPGISELISRRAMLKGMAASGAFGLF
                     GSVSGCATSGGTQSSGDGSAPLTFAEIGRTTDDKHHVPPGYNAQVLLRQGDPIRPGAP
                     EYNPAQQTGADQEQQFGTDADFISYMPLPLGSASSTRGLLGVNHENHRAQICFPGITA
                     KSQLTRQQVEVQMAAQGFSVVEIAKEGNQWRLVKDSRYNRRISTNAPMRISGPAAGSP
                     RLRTAADPSGTRSFGTFNNCAGGTTPWGTMLTAEENIQNYFSGDAGKGPEAAARKRYN
                     ISGKGRYADWGRYFDRFNIDKEPNEANRFGWIVEIDPYDPNHTSVKRTALGRAAHECA
                     THAVSHDNRVAFYSGDDARMEYVYKFVTEGKYDPANREANRDLLDRGTLYAARFEANG
                     KMRWLPLVHGQGPLTAANGFNSPADVMIEARRAGDLVGATPMDRPEDVQAHPLTGRVY
                     VVLTYNELRKPDQINPANPRANNKYGHIVEIVPPLVNGKPDHTATECDWGFFLLAGNP
                     ADASHAARYSGPVSANGWVAAPDNVAFDPKGRIWISTDGQDDMAGWNDSLYAANVSGP
                     NRGATRCFFTSPVGSEVCGPEFTPDGRTLFLAIQHPGDGSTFDKPSTRWPDFRSELPP
                     RGSVIAITRNDGGEIGS"
     gene            20454..21146
                     /locus_tag="VIG70_04015"
     CDS             20454..21146
                     /locus_tag="VIG70_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8249748.1"
                     /translation="MLTGISKGGFGGGAGGAATPLLAMVIPPAQGAAIMLPVLCVMDV
                     FGIRAYLWRWDARLVRITVSGGLIGCAAGAAAFGHLDDDWIRILVGLTALGFLAYSLL
                     PRKPLPAPSDRQGWFWCALSGFTSFITHSGGAPLMVYLLPQKLDKTVFVATSLVFFAA
                     MNYVKILPYLWLGLLDARNLATSAALLPAGVAGIYLGLRLQRRISARWFYRLIYGLLF
                     ITGVKLLYDGLT"
     gene            21197..22156
                     /locus_tag="VIG70_04020"
     CDS             21197..22156
                     /locus_tag="VIG70_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249749.1"
                     /translation="MSRLLGLVVSFLFAAGASAQAFPSKPVRILVPFPAGGTTDIVAR
                     LVGQRVSEQVGQPVLVENRAGAGGTIAAAEVARSAPDGYTLLIHNITFPIASVAAALA
                     NRATYNADADFAGVSIAVYVPFVWTANPATPGKDLRDAMSHVKQFKPYTYGSTGPGST
                     MHVLGEALSRAAGVEMTHIPFKGAAPLKQELIAGRIHLGGDQLSSSLADIKAGKLKAL
                     ATGASRRIAELPDVPTVRELGYPMLEAEGWNGFFAPAKTPREVLERLNREFATAVKHP
                     DVAKRMREMAAEPVGSSGAEQEAIFKRQMDQFRPIIKEMRIEG"
     gene            22159..23109
                     /locus_tag="VIG70_04025"
     CDS             22159..23109
                     /locus_tag="VIG70_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249750.1"
                     /translation="MRKIFLGLLFVCGTAIAQTFPTKPVKLIVTYPPGGSSDLMSRVY
                     GAKLGDLWGQQVIIESKPGAAGSIGMDFAAKQPNDGYTFVVGNLGPVAVNPLLSKVPY
                     NIEKDFIPVSMISTGPNVLVVRTDSQFKSLAEIIDYAKKNPGKLNYGTSGPGSVSHLS
                     SEMLKNGQKVEAVEVAYKGGVLAIQDLLGGQIQYIFSDSLPAMGHIKAGKLRALCVTG
                     ESRYALLPEPPLCREAAPGLVAVNWWGVLLPAGSPKSVVDKLNADTVKALADPDVKKR
                     FADLGVEAVSSTPAEFAAFIKAEMDKYAKLIKEANIKVNP"
     gene            23106..23759
                     /gene="maiA"
                     /locus_tag="VIG70_04030"
     CDS             23106..23759
                     /gene="maiA"
                     /locus_tag="VIG70_04030"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEY8249751.1"
                     /translation="MKLYGFWRSLASYRVRVALALKGLQAEEISIDLLKGRQSSPDYL
                     AVNPQGVVPALILDDGGPPLFQSIAILEYLEEIRPVPPLLPRDPRGRARVRGLALIAA
                     ADGHPLVTPRIRNYLEKEMRQDEAARNRWLGHWTMHALEAIEGHLSREKETGRFCHGN
                     TPTMADICLAGQVIGALAYFSCDTSSIPAAMRIYNQCMEIEAFAKAHPLKQPGARPH"
     gene            23759..24841
                     /locus_tag="VIG70_04035"
     CDS             23759..24841
                     /locus_tag="VIG70_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8249752.1"
                     /translation="MKPIEVAVIGTGWCGGIRAETLAAHPLVKALHLSEINKVRLQEI
                     KAKTKPKTAGTDYKELLKKAEIEAVYISATPETTHYPIARDCLAAGKHVFLEKPIALT
                     LEEADELIGLAKKSRLKFTIGYSQRFNPKFAYVRQCIRDGTIGRPVSALVSRHITRSL
                     GKKISGRVKLSPAAMESTHDLDFLLWCLEPAKPVRVYSQQNFGVMQETSGQPIADTQW
                     ITVTLDSGLTFVVGGGWTLPLGYPNFSTTWIEMVGTEGAVLVDDSHRDVLVNTVKKGM
                     QLPMSTMPGEFVEHTYAGAMAPETVHFLEAVVYDRPVLVTPEHARMVMEVYIAADLSA
                     ERNAAVDLPCEKPRSGSASRSSARAA"
     gene            24787..25887
                     /locus_tag="VIG70_04040"
     CDS             24787..25887
                     /locus_tag="VIG70_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8249753.1"
                     /translation="MRKTAKRIGLAIVGAGRVGLFRGEVAARHPMVDFIGIADLKEDR
                     LELVQKKTQADFATTDFRELLKRPEITAAIISTDEHLHVEPILAACERGISLLIEKPL
                     ATELADSQKVLDFITKKKVDAVVGYTQRFRRRWLAAKEKVRTGSLGDVTLVSSRAFMN
                     RLVALDNYKRTSDASKISPMVISGTHALDIVMWMMEAKKPVELYARSVDKALGPLCKG
                     IDATAGTLVFEDGSLYHACISWALPIVWPGAVYSLEVGITGTEGVLTIDDTHRDIVLA
                     TTKAQSEGYAPDSQRRVDFLGSYPPGDMALGELRGPMREETEQWLNRVSLGLSTQHAT
                     AAEAHNRLMLTKAFDLSARRKKPVQLPITEDS"
     gene            25884..26846
                     /locus_tag="VIG70_04045"
     CDS             25884..26846
                     /locus_tag="VIG70_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249754.1"
                     /translation="MKKLLSLLVALVVPTIAWGQYPNKAIRMIVPFAPGGASDFVGRI
                     LQPRLTELLGQQIVVDNRAGASGNIGMDAAAKSAPDGYTIYLGNVGTVALNPAVFPKL
                     GVTPTKDFVAITQVVDVPGVLVVNPELQAKTVKEIVAIAKAYPGKLNYASPGAGSQNR
                     LEMEVFRKVAGGLDMVHVPYKGGAGPAVTGLVGGETHLMFVTASSAMNHVKSGRIRLI
                     AVTSSKRLEAFPDAPTMAESGFPELTSGSWQGIFVPTGTPKEVVDKLYGAIMETMKTP
                     EVKQRLSNGGVEVVTSAPGEFAQFVAKETERWGKAVKEAGATAE"
     gene            26879..28147
                     /locus_tag="VIG70_04050"
     CDS             26879..28147
                     /locus_tag="VIG70_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY8249755.1"
                     /translation="MANAGMVALDWQQRINWDRVRKYRLERAREKMKAHGLGAMLLMY
                     DENVRYVTSTLTPGWNRLKPGLRYAMLCGDDAPVLFEQGDIGFQIQRHSPWIPKDHVR
                     YSYAWIKGAAGPASSMQVKKFTNAVKQEMKKANVAGAKLGVDFIDINMLKVFADEKID
                     WTDGMTPMMEARAVKNEDEQEALRMVGAIGDAAHWEFMSYLRPGRTENQLTAHIMEYL
                     YSVPGMEDVEDVIVSSGPNTWPNWRNFSDRIVKPGDIVFMDLAALTWNGYKSCYYRTY
                     CAGKEPSKEQKDIYATALKWLYDSIKAVRVGATTKDIASKWPSAKEAWGYEEEDQAAA
                     NLWGHGLGLAQYDQPVISRIWSLDHPIEIKEGMVFALETQHGKRFEWGVRIEEMLIVH
                     KDKIEIISNFPVEQITVVDPITGYSTLAGR"
     gene            28197..29180
                     /locus_tag="VIG70_04055"
     CDS             28197..29180
                     /locus_tag="VIG70_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY8249756.1"
                     /translation="MVREKPRPGPGEVLVRTAATAVCHTDLSIYTGAHPGVRYPVVMG
                     HESTGTVDAVGEGVGLRIGSPVIINPIIACGHCDPCRRGAGNLCRNAGLFGREVEGSL
                     SEYVKLPSQYIYELPAGLSLEKATLIETLATVRHAQHRAGLAKGESVVVLGQGTGGLL
                     HTRLAVLTGASPVIAVSRTRWKLEMAEKMGAQHVVAASAMDAVEEVKRLTGGKGADLV
                     IDAAGGAEALKAALAMLRPGGRFSAYSISHEPVHGFSTFPLYFGEISLIGSRALTAAD
                     MAPSIELVASGKVDVDGFVSAKYPLADTAEAFKEYEGNPGRILRIVIDSQK"
     gene            29275..30519
                     /locus_tag="VIG70_04060"
     CDS             29275..30519
                     /locus_tag="VIG70_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY8249757.1"
                     /translation="MTFVLPLKDSQAANAARFGSKAARLAALSHAGLPTPGGFCVDAQ
                     AYRLQLAHLGLEEAARGVFAAEDRPQARRHALSVKLGLMTGEIFPALRDDLLEIFNPV
                     VRSSALVEDKAGASFAGQFQSYLGLETEADFLTAVRACWAALWSTRALRYFAASGLDP
                     AATGMGLIIQPLVQARCAGGGLSRTADGDMLVNAAAGPGSSVAQGEVVPDRYVLDRSG
                     ALKESTVGTNYHSMGCKHGRRAFSRLLAAPRLDAEQLAELGSFMKKAEELMQGPVEIE
                     WALDDAGIKLLQARPLSVAEAPVPDEIWLKHPRLSGHPGGAGWGTGRARIVSCECELE
                     RVAPGDVLVTTVAGPALSDVFHRVAGIVAELGGSTSHLASLARERGIPMVLGAMDATR
                     RIPDGAMVAVDGVAGVVRWMRA"
     gene            30519..>31447
                     /locus_tag="VIG70_04065"
     CDS             30519..>31447
                     /locus_tag="VIG70_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEY8249758.1"
                     /translation="MAKAKVVLTDYVWESLDVEKKTLEGLADLVALKTKKPEEFLGEA
                     ADCDALLNTYAGPITAQDMARMPKCRIIARYGIGVDTIDLEAATAAGIIVTNNPTYCI
                     EEVAEQTMAMLLACARKIAFYDRMVRAGRWEVPPGKPMFRLVGQTLGLVGFGNIARQV
                     AIRAAAFGMRVLFSDPFVKEGPGKTDLEKLLAESDFVSLHPPLTPQTRKMINDEAFAK
                     MKPTAFLINCSRGPVVDTEALVRALDAKRIAGAALDTVDPEPLPNPHPLRGRENVILC
                     PHAAWYSEQAMRGLQAGAPGEVRRVLTGEWPVNV"
BASE COUNT         5600 a        10384 c        10458 g         5005 t
ORIGIN      
        1 gagcgtgtgc tgcaggtgcc gtcgcagtcc gccgcgatcc tcaccgtgct gatgctgcgc
       61 ggcccgcaga ctgcggggga gttgcgcatc aacgcggagc gcctgcacag cttttccgac
      121 atctccgcgg tgcagggctt cctggacgag ctcgctgcgc gcccggccgg ccccctcgtc
      181 gtcgagttgc cgcgcttgcc cggtgcgcgc gagaaccgct gggcgcacct cctgagcgga
      241 gctcccaata tcgagacctc taaggtcgag actgccaagc ggccagcaac ttcactcgaa
      301 gccgacgtcg ccaggctcga agccgagctc gcggccttga aggacctggt cgcccgcatc
      361 gcgaagcagg tcggcatttc gtaggtccga ggcggaaagt gatgcccgtc attgccggga
      421 ggagagaaaa ggctcagaag ggcccctttt ttgtgcgctt tctggcattt gcacaatcgg
      481 caaaaacgta gttaaatgaa ccacctccaa tacgaagtta ataaattact ggagagaagg
      541 cgagggttaa acctcctcct cgaaactcct cgcctgcagc tcacagccgg tcagtacgcc
      601 agacgagacg catccccgct ggcgccctga ccggcttttc atttgtgctc tcggctggcg
      661 ccggcctgtt ccagtgcggc ctgggcgcca tcggcgtcgc cgcgggcgcg ccgggattcg
      721 gcgatcagcc gccagttctc ggcgcgcagc gccttgtcct cgccgaccca ggagttggag
      781 cgcgcggcga cgctctcggc ctgggcgtac tgcccctgct tcaggcgcac ccgcgccagc
      841 tcgtgccaca ggcgcggatt gcgcggctcg atgcgcagcg cccgctcgag cgaggcggcg
      901 gcgccggcga ggttgccggc cgcggcctcg gtgcgcgcgc tgtccatcag gcccgcgatc
      961 gcaatattct ctttgggagc aggagcggca accggcggcg gcgcttcggg cttcggctcc
     1021 gcttccggcg cgggaccggg aggcgtggcg cagcccgcga gcaggaccgc aagaagaacg
     1081 acaagcctca tctgccgaac aaccggtcga gccagctctt cgcgcgctcc acggattttc
     1141 cgaccacctc gaccgctgcg tccatggcgc ccgagcacgg cgcgcgctcg cttggcgccg
     1201 agccctgcac gaacgggact tcagtcgcgc cgggacagct cgtgccgccg cgcagcccgg
     1261 tctgcggatc gatcgatacc atttcgatgc gctcgggcct ggcgagcacg agcggctcgg
     1321 gcgcgagcac ggacatcagg tcgccccaga ccggcagcgc ggccgccgcg cccgaaaggc
     1381 gggcggcgcg gttgtcgtcg tagccgagcc acacgatgcc gaggcgatcg ccggtgaagc
     1441 cggcgaacca ggcgtcgcgc tgctcgtcgg tggtgccggt cttgcccgcg gcggcagtct
     1501 cgggcggcag gaacctgttc aggccttgcg cggtgccttc gcgcaccacg tcctgcatcg
     1561 ccgcggcgag caggtagatc ggctcgggcg cgaacacctg ctcgaccgcg agctgatagc
     1621 gcgagagcgg ccggccttcc tgcgtggtga cgtcgcggat cgcgcgcagc ggcgagcgga
     1681 agccgccgct cgcgatggtc tggtacatct gcgccacctc gaagggcgag agctccaccg
     1741 cgccgaggag ggtcgaggcg aacggccgca gcgggcgatc gacgccgagc cggcgcacgt
     1801 tgtcgagcac ccgctcgatg ccgagatcgg tgcccaggcg cgccgcggcg gcgttgtagg
     1861 actgcgccag cgcagtcctc agcggcacga tgccgtggaa cttcttgtcg tagttcgccg
     1921 gctcccagtc cggagcgccg cggcttttcc acacgaacgg cccgtcgtcg atcggcgtga
     1981 gcagcgtgta gcgcgccggt tcgctcaagg ccgtcaggta aatggcgggt ttcagcagcg
     2041 atccgacagg ccgcgcggca tccagcgcgc ggttgtagcc gcggtagcgc gggtcgcgcc
     2101 cgccgaccag cgcctgcacc tcgccgctct gcgggtcggt caccaccacc gccccttcga
     2161 ggccggcctg ctgcccgaag cgcttctcct tgtcgtactg cgcgaggcgc ttggcgagcg
     2221 cggtctcggc cgcgtcctgc acgcgcgggt cgagcgtggt gaagatgcgc aagccctccg
     2281 agcgcaggtc ggcctcgtcg tagtccctcc gcagctgccg gtgcacgagc tgcaggaagg
     2341 cggggtaggg tgtggtgccc gtgcccgttt tcttgctgac attcaagggg ctgctgcgcg
     2401 cgtcggcata ttcgtccggc gtgatgccct tctggtcgcg cgtttcgcgc agcaccaggt
     2461 tgcgccgctc gagcgcgcgc tgcggatggc ggtaggggtc gtagatcgcg ggccctctca
     2521 ccatgccgac gagcagcgcc gattcggccg ccgtcaggta ctgcacctcc ttgccgaaat
     2581 agaactgcgc ggcgagcccc acgccatgga tcgcgcggtc gcggtcctgg ccgaaataga
     2641 tctcgttgag gtaggtctcg aggatctcgt tcttgtcgta gtggatctcg agcagcaccg
     2701 ccatcaggag ctcggtgaac ttgcgctgca gcgtgcgttc cggcgtaagg aagaagttct
     2761 tcacgagctg ctgcgtaatc gtgctgccgc cctggacctt gtccgagaac acgctgcgcg
     2821 ccgcgcgcgc gaggccgcgc aggtcgaagc cggcgtggga gaagtagctg cggtcctcga
     2881 ccgcgatcag cgcttggacg aaatgcttcg gcacgtcgcc caggcgcacc agcacccggt
     2941 cctcgttccc ggccgggtag atgccgccga tggccagagg ctcgaggcgc gcgagcgcga
     3001 gctcggcgcc gcccgtgtcg cgcaacgcag caattctgtt ctgatcaaaa acaacggtta
     3061 cccgcttcgc cggctgtttc ccgtcccaga acacgaacgg ccgcagcgcg atctcgagcg
     3121 aatcgtcctt cttggcgaac cagcccgctt cgccctcggg cggcgcctcc cggtagccga
     3181 ggctgcgcag ctcgtcctcc acgtccgcct gcgcgatgcg cagcccggcg tgcagctcga
     3241 gcgggcgggc gtagatgcgc gccggcagcg cgaagcgccg gccctcgaac tcgctgcgca
     3301 cccggaagtc gagatacagc gtaaaccccg ccaccgcgag cacggtcgcg agcagcgcga
     3361 ggcgcacgac tttccagagc ttcgggcggc gacgcagcat cgcgtcgatt ctaaccgtcc
     3421 aattcgggca aaatccttat gccgtgctga ccattcgtgg catcgatcac gtcgtcctgc
     3481 gggtacgcga catcgaccgc gtgcgtaagt tctactgcga cgtgctcggc gcgcaccacg
     3541 tggcctaccg gcccgagctc ggcatgtcgc acctgcgcat cggcgccgcc atgatcgacc
     3601 tggtcaaggg cgagggaccg accggctgca acatggacca cttctgcctg cgcgtcgagc
     3661 cgttcgacca ggacgcgatc gtggcccacc tcaagaagca tggcgtggcg gtcggcgaca
     3721 tccggaaccg cttcggcgcc gaaggcaacg ggatttcgat ctaccttacc gatccggagg
     3781 gcaacaccgt cgagctgaag gggccctcgg acggaaccgc tcagccgccg acgcctaaat
     3841 aggtcgtcgt gacctggcgg tccgcgcgca gcgcggcggg cgtgccctcg aagaccacgg
     3901 tgccgtggtc gatgacgtag acgcggtcgg cgagctgcag cgccatctcg gcgttctgct
     3961 cgacgagcag catgctcacg cgctccttct tcagctcctg gatggtgctc atcaccgcgc
     4021 tcaccacgag cggcgcgagt ccctgcgagg gctcgtcgag caggaggagg tcgggtccgg
     4081 tgagcagcgc gcgggcgatg gcgagcatct gttgctcgcc gcccgagagt tgccgtcctt
     4141 tgttgttgag ccgctccttc aggctcggaa agcgcccgag gacgttgtcc atctgccatc
     4201 gcgactgggg acgcctcgca atggccaggt tctcgcgcgc ggtcaggtac gaaaagatgt
     4261 cccgggtctc cgggaccagg gcgatgccga ggcgcgagac acggtgcgca tcgaggccgg
     4321 cgatgtcgcg gcccttgtac cggatctcgc cgctcttcgg cggcgtgagg ccggtgatcg
     4381 aacgcaaggt cgtcgttttt ccagcgccgt tccggccgag cagcgcgacc acctcgccct
     4441 cggccacgct cagcgagagg tcgtgcagga tgtgcccgag gccgtaaaac gtattgatct
     4501 tcttcagctc gatcatgccg cgatgccgcc caggtaggcg cgccgcacct cggcgttgcg
     4561 ctgaatttcc tcgggcgcgc cctcggcgat gagcgagccg aagtgcatca ccgagatccg
     4621 gtcggacacg ctcatgatga tgtccatctt gtgctcgatg agcacgatgg tgtagcgccc
     4681 cttcagcgtg cgcaccagcg ccaccatgtt gcgcgtctcc tcgggcgaga gcccggccgc
     4741 gggctcgtcg agcagcagca cgcgcggcgc ggcggcgagc gcgatggcga gctcgagctg
     4801 gcgctgctcg ccgtgggaga tctcccgggc cagctgattc gccttgctca gcaggcccac
     4861 atcggagagc gccgcgcggg cgcgctccat gacctcggga taccggtccg tttttctgaa
     4921 cagcaagccg ttccaggagc ggccggtcgc aaaagccgcg acccagacgt tgtcgagaag
     4981 cgaaaaggcc gggaaaacgt tggtgcgctg gaacgagcgc gcgaggccga ggtgcgcgat
     5041 gcggtcggcg cgcgtgcccg taatattttt cctgtcaaaa agaatttccc cggaggtcgg
     5101 cgggaaggtt ccggtcagca ggttgaagag cgtggtcttg ccggcgccgt tcgggccgat
     5161 gaccgagtgc aggctgcccg cagcgatgct gaggctcacg tccttcaccg ccgccagggc
     5221 gccgaaggat ttgctcagct ggcgggtttc gaggatgcct tccatgccca gaaccggttc
     5281 catgcttcca cgacgccgcc gcggaagaac agcaccacga tcacgaacgc cacgccgagg
     5341 agcagcggcc agcgcgccca gatgacgctc accgactcgg agagccagat gaagacgaac
     5401 gagccgatga tcggcccgta gagcgagccc gagccgccga tcagggtggc gaacaccacg
     5461 ttgccgctga atacgaagct gatcgcctcc agcggcacga tgccgaacag catgccgtag
     5521 agcacgccgg cgaggcccga gaaggcgccg ccgatgacga acgcaagtag cttgaagcgc
     5581 ggcacgttgt agccgatggc ttcggcgcgc acttcgttct cccggatcgc ctgcaggatc
     5641 ctgccgagcg gcgattcgac gatccgccgc atcgcccaca gcgacgccag aaacagaagt
     5701 gagatgaaga cgtagtaggt catcgggtcc ttgaaattga ttccaaggag atccggccgc
     5761 ggcacgccgt gcatgccgtc ctcgccgccg gtgaccgtgg tccactggta ggcgacgaaa
     5821 tagaagagct ggttcagcgc gaaggtcagc atgatgaagt acagccccga gcgctgcacg
     5881 cacaagtagc ccaccagcag cgccgagagc gcgcccacgc cgaccccgat accgagcgcg
     5941 aagaacacgt tgatgccgta gtgcttgagg agccaccccg ccacgtacgc agccgagccg
     6001 aagaaggtcg actggccgaa ggaaagcaag ccggtgtagc cgagcagcag gttcagtccg
     6061 agtccgaaga tggcgaagat ccagatctcg gaggcgatga cgggacggac gatgaagggc
     6121 agggcaatga ccgctgcgac gagaagaatg ttctggtatt tcatcgaatg ccgaacaagc
     6181 cctgaggccg aaccagcagc accgcggcca tcaccgcgaa gatcaccgcg gtggaggcct
     6241 cggtccagta cgccgcgacc accgcctgcg cgacgccgat cagaagcccg gccgcgatcg
     6301 cgccgagcag gttgccgaga ccgccgagcg ccaccacgac gaaggcgatg ccgagcatat
     6361 ccacgcccat ctggggactc agtccccgaa tgggcgccgt cagcgcgccc gcgatgccgg
     6421 cgaggtaggc gccgagcgcg aaggcgacgg tgaagagttt gtggatgtcg atgccgagga
     6481 gcgacaccat ctccttgttc tcgatgccgg cgcgcatgat cgcgccgtag cgcgtcttct
     6541 cgaggaacag ccaggtcgcc gcgaccagca gcccggccac gaccatcacg aacaggcggt
     6601 acttcggata gaacgtgaag ccgaggtcga ggccgccgcg caggatcggc ggcggcagat
     6661 ggctgtagcc gaccggtccc cagacgatga tgattgcttc cgacaggatc agggcgaggc
     6721 cgaaggtgaa aaggagctgg tagtcgtgtc ccgccgagta gaggcgccgg atacccgcgt
     6781 actcaatcac caggccaaga atggctacgc cgatgggcgc gatcagcaac gccagccaga
     6841 agttgccgat tacgttcacg gtgctgacga gaagataggc cccgacggcg aacagcgcgc
     6901 cgtgggcgaa gttggggatg ttgagcaccc ccgtgatcag cgacaggcca agccccatca
     6961 gggcgtacag catccccgtc gcgatcccgt tgaggatctg actgaggagg aggttcagaa
     7021 actaccgatg tccttgcagg ccgcgtactc cttcggcagc ggcgcgtctc ccgtcgcgat
     7081 gatgtcggcg aagtccatcg ggtgcttcat cgccgacttc ggcttgcagc gcatgaagaa
     7141 gtacggccgg atggtctggt gcgtcgccgg gtcgacccgc atgcggccga ggaggtcgtc
     7201 cacctcgatg ccttccatcg ccttgaccac gtccgccacc tcgccgatct tcgacttgtc
     7261 catcgcggcg agcgtgagcc gcgtcatcga gtaggcggcc gagggcgcgt atcccggcgg
     7321 gctgccgaac ttgccctggt agctcttgac gaactccttc gccacaggcg tgtcggcggt
     7381 gtagtagaag ttagagccga cccagatgtt ctcgcggatc tcgggggcga gctgcaccag
     7441 ctcctcgacg cccgaggacc aggtcatgat cagcggcacc ttgggcgtga ggccgaagtt
     7501 gaagatctcg cgcgcgcccg acaccgcgtc gagcccgaag ttgatcatcg ccaccgcgtc
     7561 gggccggttg gccgccgcct tctgcacgta gttggagaac tcgcgctcgc cgagcggatg
     7621 gcggtccgcg cccacaaatt caatgccgtg ggtcttgccg acttccttga tgtatttctc
     7681 taccgaccag ccgaacgcgt agtccgccac cagcaggtgc cagcgcttcg ccttcggatt
     7741 ggccttcatg aaattgtcca ccaccgtttt cgccgcggtg tacgggctcg ccgcccactg
     7801 gaacgagtag cgatggcaac gcgcgcccga gatgtcctcg gtgccgccgc tgaagatgtg
     7861 gaacaccttc ttgcgcttcg cgacctcgga gaccgcgagc gccacgcccg aggaccacgg
     7921 gccgatgacg aacttcgcgc cctcgccgtc gaccgcctcc tcgacgcgcc gcgtcgcggc
     7981 gccggcctgc gtcgccgagt cgcgcacgat gtagcggatc ggccgcccga ggatgcgcat
     8041 gttgcgctcg tcgagcgcca tcttcatccc gcggtcgagg atcgggccga cgtcggcgaa
     8101 cgcgccggtc gcgggccaga tcccgagcat cacgatctcc ttcggctgcg cgcggacgat
     8161 ggcgggaaag ccgagcgccg cggtcgctgc gccgagcttc aggattttcc tgcgcttgga
     8221 attgctcatc ggtgaccctc ctctgggcgg cagcgtaccc ttttcatgtt aggttgcaac
     8281 caccttgagg cgctggatcg ccatcgcggt tcttcagctc gcggccgggg ccgcgcaggc
     8341 gtgcttgttc gcaaccagca cgccgcccga gggctggtat cagtgggcga gcgggctgtt
     8401 tgcgggcgac gtcaccggcg tcgcgaagga cggcgaaaag ccgatcgatg tcgtcacggt
     8461 tcgcgtcgtc gagaccttca aggggccgga cgcatcgcac ggcacgctca cggtgcgcct
     8521 ctcgaaccgc tactggacca actgccgcgt ggcgcggccg gctgccgggg cgcgcgtgct
     8581 ggtcgccatg aatgcgaaca gcgacgccat gctcgtgccg ctgtcggaga gctacgccga
     8641 gcagctcagg cgacaacgtg cgataggggt acgatagccc atgatcaaga ccgccacggt
     8701 gtttgccatc gtgctttccc ttgccgccgc cacggctgcg gctcagaacg acagcgggaa
     8761 gaagaccagc gacggctttg gcaacctgtt gaagggaatg ggccaggaga tcaacaaggc
     8821 tggcggcagc tctactgcgc cgaagaaaga tgccaagaag gcggacgcca agaagtcgga
     8881 cgccaagaag aaggatgcca aggcggcaaa gaaggaatag gggagcgggc tagatcacga
     8941 cgtgcgaggc ggcgccgtcg gtgctgatgt tggcgcccgt caggtagcgc ccgcgctctg
     9001 aggcggcgaa caccacgatg tcggcgattt cctcgggctc ggcgggacgg cccatgggga
     9061 gacgggcgac catctgcttc tccgcctgct cgacagagac gttggctcgc ttcgcttcgg
     9121 ctttcagccc ttcggccact cgctcggtct ttgtgacgcc cgggctgacg ccgacgaccc
     9181 gcacaccctt gctcgcatag gcataggcaa ggccggcggt ggcgaggaga agcgctgcat
     9241 tggccgcgcc accggcgagg tgcgtcggcg acgcgacctt gccgccgctg ccgatcacgt
     9301 tgatcaccgc gccgcgcccg cgcttgccca tgcgcttgat caccgggtcg atcacgttga
     9361 tgtaggagaa gtacttcgcg tccatcccgg cgcgccaggc cgccggcgtg aggtcgtcgg
     9421 cgtcggtgcg cttcgcggcg cctgcggaat tgaccaggac gtcgatcggg ccgaattcct
     9481 tctccacctg ctcgaccatc gccgcggccg ccgcggcgtc ggtgagatcg gcggcgacgg
     9541 tatatgcgcc tttcaatttg tctcttgctt tattcaagtt ctcttcagaa cgcgagacca
     9601 gggcaacgcg cgcgccctcg gccacgaacg cacgcgcaca ggcgaggccg atgcccttgc
     9661 tgccgccggt gatcagcacc ttcttgtctc ttaatccgag gtccatgaca gttattccgc
     9721 ggtgattttt cggttcttga tgagcgtgcc gaagcgcttc acctcggacg cgatgaactt
     9781 ggcggcgtcc tccggcgtgg tgatgaaggc gtcgaagccc tgctgggaga acttgtctcg
     9841 cacgtccttg gaggtgagtg tttttctcaa gccttcattc aggtgtgcta cgacctccct
     9901 tggcgtgccc gcgggggcga gcagcacgag ccactgggtg aactggaagt ctttgacgcc
     9961 ggcttcctgg agcgtgggca cgtcgggaag cagcggcgag cgcgtggtgc tcgtgacgcc
    10021 gagcgcgcgc agagtgccgg ctttcacgtg cggcagcgcc gccgacagcg tcgggaagct
    10081 catgtctacc tgcccgccga gcgtgtcgag cagcgccggc ccgccgccgc gatagggcac
    10141 gtgcaggatg aaggtgctgg tcgagtcctt gaacatctcg gcgcagaggt ggaaggtggt
    10201 gccgaccccg gctgacgcgt aagtcagctt gccggggttg gtgcgcgcga gccttatcag
    10261 atcgtccacc ttgctcgcat gtactttcgt cgggaccacg agcacgtggg gcgcggtcgc
    10321 caccattccg atcggcgcga agtccttcac cgggtcccag gagagcttgt cgaacagcgc
    10381 ttggttggtc gcgagcggga tggtgttgag caccacggtg taaccgtcgg gcttggcgcg
    10441 cgccgccgcc tccatgccga tgttgccgga ggcgccgctg cggttgtcga tcacgatctt
    10501 gccgccgagc tgccccgcca gcggctcggc gatgagccgc gccatgatgt ccacgctgcc
    10561 gccgggcggg aagggcgcga tcagccggat cggctggctc gggtagggtt gcgcggcggc
    10621 tgggagggcc cacagtaacc cgagcgccag aaacacgatc ctcagcatgg ttcccccttg
    10681 gaatgttccc gaacggacaa tgatagcggc tcgtttcaaa cgtgagcccc aaaactgttg
    10741 cgtgcgcgat acaccttgtc gttatttcgc atcgctcggg gcgcggtaag cgtagagttc
    10801 gcgctcaacg ggggagccct ctaactcaca cgaagggaga agtatgcagc gcaagcttct
    10861 agcagcagcc ttggcatccg cctttgcaat gcccgcattt gcccaggtgc cgcccgagct
    10921 caagatctac gggcgcgtca acgtctccat ggagcgcatc gagaccgaca acagcaccaa
    10981 cccggcggtg ccgaaccagt cgaactacga gttcgtggac aactcttcgc gcatcgggtt
    11041 tgcctacaac aaggatctca cggcgggcct gaaggcgatc ttccagatcg agagcaacgt
    11101 caacgtgacg gatggcggac aaaccttctc cagccgcgac agcttcgcgg gcctgcaggg
    11161 cggcttcggc accattcgcc tcggccgcac gattggtccg gtctactacg cgacctacga
    11221 ttacatcagc atgcacaacc acgacaccgg cacgtcctcg gacgcgcttc tcgccggcac
    11281 cgtggtcggc aaccagggct tcatgaacaa cacgctctgg tacaccagcc cgaagatcgg
    11341 caacttcacg atcgaaggcg cgttctcgct gctcggcgag cagcgtcaag ccccgggcat
    11401 ggatcagccg cgacacatcg gcctcgtcgg cgcctacgac acgggcccgc tgcacgccgc
    11461 gatctcgtac gcgaacacga agaacgacgc tgatcttggc ggcggccggc cgagcgacgc
    11521 caccgcgtac accttcggcg gcgcctacga tttcaagttc atggtgctcg gcgcgctgtg
    11581 ggagatggcc gagcgcgaca tcgcgaccgg cagcgtcgac agcgactact ggcgaatcgc
    11641 cgcgatgttc ccggtcggcc agcacgagtt ccacgtgaac tacggcctag tggatgccga
    11701 cggcgaccgc ggcgcgcagc aatggacgct cggctacaac tacaacatca ccaagctgac
    11761 caaggtgtac gcgttctaca cgatggtcga taacgatacc gccggcaact ttacgatgaa
    11821 cagcacgagg acggccgggg cggccaccgg cgcccagtac acgtcgatcg cggtcggcct
    11881 gcgccacaac ttctaaagcc agctgttcag caatagcggg gacgggcctt cgggccggtc
    11941 cccgtttttt ttgcccgctc gggcataatc ggcccgagga ggagggccgg gcttgaaggc
    12001 gccgtcgttt gcttatgcga aaccgcgttc gctcgcggag gcgttcgacc tcctcgagcg
    12061 tcccggcgcg aagattctcg cgggcggcca gagcctgatc ccctcgctca acctgcggct
    12121 gtcggcgccc gagctgctgg tcgacatcac gggcatttcg gggttttccg gcatagcgct
    12181 cgcgaacgga tctctccgaa ttggagccct atgcacgcac gcgcagcttg agaaatccga
    12241 ggatgtcagg aagcaccttc cgctcgtagc ggaagccgtc tcccatatcg cccatccggc
    12301 gatccgcaac cgcggcacgc tcggcggcag cctggcgctc gccgacccgg cggcggagct
    12361 gccggcgtgc gcgctcgcgc tcgacgcggt gctcgtcgtc gcggcgaaaa agggcgagcg
    12421 gcgcgtgaaa gccgccgact tcttcaaagg actgttcgac accgacctca agcccgccga
    12481 gatcctggtc ggcgtcgaat ttcccaaggc cgacaagtca gtcttcctgg agctgacccg
    12541 ccggcagggc gattacgcga tagttggtct ggcggcagta acgagagctt ctcaaaaaag
    12601 aatttcgttt ttcggcatcg ccggcaagcc ggttctcctg acgcccaagt cgctcgacga
    12661 ggcgaaagca tccgtgccgc cgccggcggc agacctctac cactcctcat ccacgaagct
    12721 gcacctcgcc aaagtcctgc tcgatcgcgc gtggaacaga ctctgaccac caccatgacg
    12781 gtgaacggca cgcaggtgac gcgcagcgtc ccgccgcgcc agcacctggt cgatttcctg
    12841 agggaggagc tgggcctgac cggctcgcac ctgggctgcg agcacggggt gtgcggcgcc
    12901 tgcacgatca gggtgaacgg ggagatcgtg cgcggctgcc tgatgctcgc agtgcaggcg
    12961 aacggctgcc gcgtcgacac gattgaaggc ctgtcggatt cgaaagagct ggaaaagctg
    13021 cagggcgcat ttcatcgccg aaacgccctg caatgcggtt tctgcacccc ggggatgctg
    13081 atggccgcgc aggacctcat ccgcagcggg aagaaagcga cccgcgagga aattcgcgcg
    13141 cacatctcgg gcaactactg ccgctgcacg ggataccacg cgatcgtcga cgcgatagag
    13201 gaggtaatac aggcagaggt gaatcatggg taaggccgag cttccgctcc acgcgccggg
    13261 cacccaggtc gacaagggct acatcggcaa gagcgtgccg cggccgaacg cgcagcgcct
    13321 gctgcaggga cgcggcacct tcaccgacga cctgcgcttc ccgcgtctcg cccacgtggc
    13381 gttcttcagg agtccgtacg cgcacgccag catcaagaag ctcgatttct cgaaggcgat
    13441 caagtcgccg ggcgtgatcg cggtattcga cggcccgaca gtcgccgagt actgcaagcc
    13501 gtgggtggcg gtgctcgcgc acctgaaggg catcaagtcg ccgccgcagc acgcgatcgc
    13561 gatcggccga gcctgctggc agggcgagcc ggtcgcggcg gtggtggccg agtcgcgcag
    13621 ccaggctgag gacgcggtgg cgctgatcga ggccgagtgg gaagaactac cggtcgtcgt
    13681 cgacatggaa gattctctaa aaaacaccaa aacaatccat ccggagctcg gggacaacat
    13741 ctgcttcaag cgcgagcacg acaccggtgg ggtggacgaa gcatttgcga aggcggacgt
    13801 cgtggtggag gacacgttcc acttcggccg ccacaccggc gtgtgcctcg agggtcgcgc
    13861 catcctcgcc gactacaacg ccgcggagca ctcgctcacc gtctaccacg cgacgcaagc
    13921 gccgcacatg atgcaggata ttttctcgcg ccacttgggg atccccgagg ccaacgtgcg
    13981 cgtgatcgcc aaggacatcg gcggcgccta cggcatcaag gtacatgtct atcccgacga
    14041 gatggccacc gcagcgctct cggtgatgct gcgccggccg gtgaaattcc tcgccgaccg
    14101 gctggaatcg tttctttcgg acatccacgc gcgggagcac cgggcgcggg tgcgtctggc
    14161 ctgtaccaag tcaggcgaaa tcctggcgtt cgagctggat gacctgactg caatcgggcc
    14221 ctactcggtc tatccgcgca cgagcggcat cgaaggcaat caggtagtga acctcaccgg
    14281 cggaccctac aagcaccaga agtacaaggg ccgcaccaac gtggtgttca cgaacaagaa
    14341 cgtcacctgc cagtaccgcg cggtcgggca tccgatcgcg gtcgcgctca ccgagggcat
    14401 cgtcgacctg gctgcgcaga agctcggcat ggacccggcg cagttcagga aagtgaacct
    14461 gataccggac gacgcctatc cctacacgtt cccgtcggga ctcaagttcg agaagctctc
    14521 gcaccacaag gctttggatc ttcttctgaa gatcaccgaa tacgacaagt tgcgtgcgga
    14581 gcagtcccgg ttacggaacc agaagatcta ccgcggcatc ggcctcgcgg cgatgatcga
    14641 ggtgaccaat ccctcgccgg cgttctacgg cgtcggcggc gcgcgcatct cggcacagga
    14701 cggcgccacg ctgcggctcg agccgactgg catggtgacg gtgctgtgca gcgtcaccga
    14761 gcaggggcaa ggcaccgagg gcatctttgc gcagatcgcc gcaagcgccg tcggggtctc
    14821 catcgagaaa gtcaaagtga tcaccggcga caccggagtc acgccttacg gcggcggcac
    14881 ctgggcgtcg cgcggcgccg gcatcggcgg cgaggccgtg ctgcaggcgg gaagagccct
    14941 gcgctcgaac atgctcgacg tggcttcgaa gattctgaac ctggacaagg cggagctcga
    15001 catccgcgac aacgccgtaa tcaacaagct cagtcttgaa aggaaacttg aacttgccga
    15061 gcttgggcgc atcggctact tccgccccga cacgctgccg gcggactacc agtcggagct
    15121 gaccgtcacg cgccactaca cgccgcgcca gtacggcttc accatcacaa acggcatcca
    15181 ggcctcgctg gtggaagtcg atccggagac cgggttcgtg aagctgctga agcactggtg
    15241 cgtggaggac tcgggcacgg tgatcaaccc gatgctggtc gacgagcaga tccgcggcgg
    15301 cgtggtgcag ggaatcggcg ccgcgctcta cgaggaatgc ctctacgggc ccgacggcca
    15361 gctcctcaac ggcaacatgg cggactacct ggtgccgatg tcgggcgaga tgcccgacat
    15421 cgtgtgtgcg cacatcgaga cgccgacgct ccagtcccag ctcggcgcca agggcgtggg
    15481 cgaggccggc accgccgggg ctcccgctgc ggtgatgaac gccatcaacg acgcgctcgc
    15541 gccgctcaat gcccgagtaa cccgaatgcc gtttaccccg gagcgaattc ttgccgccct
    15601 agggaaggtc tagaaggtca ggtaaggcgt ttgttcgcct gctcgagcat catcccatgg
    15661 agatcgtcag gtggccggct tggcgacctt tggccttcgg ccggatcaag atctcgacat
    15721 cgcgatcgag ccgggtcagg aagcgcagca gccgctcgga cgagaagcgg ctcagcttgt
    15781 agttcttgag ctccgaaaca tgcggctggg acacgccgag cagctctgaa gcctttgact
    15841 gcgtgaggcc gcgctccgca atcaggtcgt tgattcggat ggcgagctga acccgcagcc
    15901 tgcgttctcc cgcatccgcg aagccaagat cgacgaaaac atcgcctgat ccggggtcga
    15961 ccttctcacg ctttctcgcc atagcgcacc tcgtaatctt cccgcgccgc cttcagccgc
    16021 cgctgcacca gttctacgtc cgtctgagcg gttcggattc cggaaggcga cttcttctga
    16081 aagcaatgca gcacatacac tgctttgcgg aaccgcactg tgtacaccga tcgccatgtg
    16141 ccgccacggc gatcctggac cagctcgaat actcccggcc cctcaccttt ccatggtttc
    16201 gccgaagctg gcgttatgcc tgcttgcacg acgccgagcg catatccaag cccgtccacg
    16261 acatcatccg gaaactcaag gagatccttc ttcgctgacc ccatccagtg gagtggtttc
    16321 tccggcagcc ctaatgtccg agccatgttg agtataccgt attcggtata gcgtgatgtt
    16381 tgtcaataca acgtctgagc gacacgacgg atgacagcgc gtatcgctcc ggtgtctaat
    16441 tcgccactac ccgagtagtc acgacccaat ggacaaccgc gtttcctcag ccatctcaca
    16501 ctgggcgccg cgcttcgtct ccaacggcgt gctgctcgcc gacttcgagg acgtcaccgg
    16561 gtcgctcgag cgctgggagg actggtgcgc ggcgtggagc gcgcgcgcca gggtgcatga
    16621 ggatctcggt cgggcttcgc tgaaggaagg ccacaagctc agcgcgagcg agcatctcat
    16681 ccgcgcggcg atctactacc acttcgccaa gttcgtgttc gtgcaggacc cgaagcagat
    16741 gcgcgccgcg cacatgaagg cggtggcgtg ctatcgcgac ggaatcgcgc tcgcgaggcc
    16801 gccgggcgag cgcgtggaga ttgcgttctc ggggaagacc atcgcgggcg ttctgcgaaa
    16861 gcccgggagc cccaagccgc cggtgctgat catggcgccc gggctcgact cgaccaaaga
    16921 ggagatccat gcctacgagg agccgttcct ggcacgcggc atcgcggtac tggcgatcga
    16981 cgggccgggg cagggcgagg cggagtacga catcccgatc tgcggcgact acgagcgcgc
    17041 ggctaaagcc gtcgtcgatt ggatagagaa gagggccgac ttgaatgcaa accaggtcgc
    17101 gatctggggc gtcagcctgg gcggctacta cgccccgcgc gccgcggcct acgagaaccg
    17161 catccgggcg tgcatcgccc tgtcggggcc gttcgagtgg gagcgcatct gggacgggct
    17221 gccggagctg acgcgcgaga ctttccgggt ccgcagccac gcggcgaacg aaacagaggc
    17281 gagggaaaag gcgaaacagc ttacgatgaa ggaagcggcg ccccggatca cctgtccgat
    17341 cttcatcgtc acgggaaggc aggaccggct ggtgccggct gcgcacgcgg agcagctcgc
    17401 caaggcggtg tcgggcccgg tcgagctcct gatcgtcgag gacggcgggc acaatgccaa
    17461 caatcgcccg taccgctatc gcagccgcac cgcagattgg ctggcgatgc agttcggact
    17521 accaaaggta tgaggaggac gcgatgagat ttctggtggc agtgctggta ctcgcgcttg
    17581 gcgctgtagc gcaggcgcaa acgtttccga gcaagacggt gcgcttcatc tcgggggtca
    17641 cgccgggaag cgcctccgat accatggcgc gcatcctggc ggaaaagctc ggcgcgtcgc
    17701 tcggccagcc ggtgatcgtc gagaaccgcc tcggcgccgg cgggctggtg ggcgcgaagt
    17761 gggtcgccac gcaggagccc gacggccaca acatcatgat gtacgcctcg gcgttcacgg
    17821 tttcgacgct cctcaacacc gggctcgatc caaaggagct ggtgccggta gcgacggtcg
    17881 ccacgatccc gaccatcctc atcacgccgc cgggaaaata caagtcggaa aaggactttg
    17941 tggctgccgc caaagcgcaa ccgggcaagc tcgtctgcgc caccgcgggc gtgggcagcg
    18001 cgacgcacat ggcgctcgag cgcttccgct tcgccgccgg cttcgaagtg ctgaacgtgc
    18061 acaccaaagg cgcgggcgag gcgctgaccg aggtgatctc ggggcgcgcc gactgctact
    18121 ttgcgctcgt attccaggcg cagaagatga aggacgccgg caaggtcgac gcgcacctcg
    18181 tgagcgggcc caagcgcacc tcgctcatgc ccgcgctccc gaccacggaa gaggcgggat
    18241 acccgggctc gtcctacaac ttctgggtcg gcacgctggt cgcggccaag acgccgcgcc
    18301 cgatcgtcga gcgcctggcg aaggagatca acgcgctcgt gcaatcgccg gagatctccg
    18361 atcgcatcac aaagctcggc gccgatccgc tcacgctttc gatcgcggag ttccaggcaa
    18421 tgatcgaccg cgagctcagg gacaatgcgg aactcatcaa gaaagcaggg ataaaggcca
    18481 attgacatga aaaaagactt cttcgaagag caggacggcg tcgccgtcaa tccatccagc
    18541 gagcccggga tctcggagct catttccagg cgcgcgatgc tgaagggaat ggccgcgagc
    18601 ggggcttttg gcttgttcgg ctcggtcagc ggttgcgcga cttcgggcgg aacgcaaagc
    18661 agcggcgacg gctcggcgcc gctcaccttc gccgagatcg gccgcaccac ggacgacaag
    18721 caccacgttc cgccgggcta caacgcgcag gtgctgctgc gccagggcga tcccatccgc
    18781 ccgggcgcgc ccgagtacaa cccggcgcag cagaccggcg ccgaccagga gcagcagttc
    18841 ggcaccgacg ccgacttcat ctcctacatg cctttgccgc tcgggtcggc gagctcgacg
    18901 cgcggcctgc tcggcgtcaa ccacgagaat caccgcgcgc agatctgctt tcccggcatc
    18961 accgcgaaga gccagctcac gcgccagcag gtggaagtgc agatggccgc gcagggcttc
    19021 tcggtcgtcg agatcgcgaa ggaaggcaac cagtggcgcc tggtcaagga cagccgctac
    19081 aaccggcgca tctcgaccaa cgcgccgatg cgcatctccg ggccggccgc gggaagcccg
    19141 cgtctgcgca ctgccgcgga tccttccggc acccggtctt tcggcacatt caacaactgc
    19201 gccggcggca ccacgccgtg ggggacgatg ctgacggcgg aagagaacat ccagaactac
    19261 ttttccggag acgccggcaa ggggcccgag gccgcggcgc gcaagcgcta caacatcagc
    19321 ggcaagggcc gctatgccga ctggggccgg tatttcgacc gcttcaatat cgacaaggag
    19381 ccgaacgagg cgaaccgctt cggctggatc gtggagatcg atccgtacga tccgaaccac
    19441 acctcggtga agcgcaccgc gctcggccgc gccgcgcacg agtgcgcgac gcacgcggtg
    19501 agccacgaca accgcgtcgc gttctattcg ggcgacgacg cgcgcatgga gtacgtctac
    19561 aagttcgtca ccgaagggaa gtacgacccg gcgaaccgcg aggcgaaccg cgatctgctc
    19621 gatcgcggca ccctctatgc cgcgcgcttc gaggcgaacg gcaagatgcg ctggctgccg
    19681 ctggtgcacg gccaggggcc gctcaccgcc gcgaacggat tcaacagccc ggcggacgtg
    19741 atgatagagg cgcggcgtgc gggcgatctc gtcggcgcca ccccgatgga ccggccggaa
    19801 gacgtgcagg cacatccgct caccgggcgc gtctacgtgg tgctcaccta caacgagctg
    19861 cgcaagccgg accagatcaa cccggcgaac ccgcgggcga acaacaagta cggccacatt
    19921 gtcgagatcg tgccgccgct ggtgaacggc aagccggatc acaccgccac cgaatgcgat
    19981 tggggtttct tccttctcgc cggcaacccg gccgacgcat cgcacgccgc gcgctacagc
    20041 ggcccggtca gcgcgaacgg ctgggtcgcc gcgcccgaca acgtcgcctt cgacccaaag
    20101 ggccgcattt ggatctcgac cgacggccag gacgacatgg ccgggtggaa cgacagcctc
    20161 tacgcggcca acgtgagcgg gccgaatcgc ggtgcgacgc gctgcttttt caccagtccc
    20221 gtgggctcgg aggtctgcgg gccggagttc acgcccgacg gcaggaccct gttcctggcc
    20281 atccagcacc ccggcgatgg ctcgactttc gacaagccct cgacgcgctg gccggacttc
    20341 cgcagcgagc ttccgccgcg cgggtcggtg atcgcgatca cgcgaaacga cggcggcgag
    20401 atcggaagct gatctcggac ccctggttct gggcgatcgc gctccccgcg gtcgtgctga
    20461 ccgggatttc gaaaggcggc ttcggcggcg gcgccggcgg ggccgccacg ccgctcctgg
    20521 cgatggtcat tccgccggcg cagggcgcgg cgatcatgct gccggtgctg tgcgtgatgg
    20581 acgtgttcgg catccgcgcc tacctgtggc gctgggacgc tcgcctggtg cgcatcaccg
    20641 tgagcggggg cctgatcggc tgcgccgcgg gcgcggctgc cttcgggcac ctggacgatg
    20701 actggatccg catcctggtg ggtctcaccg cgctcggctt tctcgcctac agcctgctgc
    20761 cgcgcaagcc gctgcccgcg ccgtcggacc ggcagggatg gttctggtgc gcgctgtcgg
    20821 gcttcacgag cttcatcacg cactcgggcg gcgcgccgct catggtgtat ctcttgccgc
    20881 agaagctcga caagacggtg ttcgtcgcca cttcgctcgt gttcttcgcg gccatgaact
    20941 acgtgaagat cctgccctac ctgtggctcg gacttctgga cgcgcgcaac ctcgccacct
    21001 cggcggcgct gttgccggcc ggggtggcgg gcatctacct cgggctgcgc ctgcagcggc
    21061 gcatcagcgc gcgctggttc taccgcctga tctacgggct gctcttcatc accggagtga
    21121 aactgctcta cgacggattg acctgaaact gggagcggta agctagacta gccgccgcat
    21181 cctggaggag ggcgcgatga gcagattgct gggccttgtg gtgtcgtttc tattcgccgc
    21241 gggcgcatct gcgcaggctt tcccgtccaa acccgtccgg atactcgtgc cgttccctgc
    21301 gggcggcacc accgacatcg tcgcgcggct ggtcggccag cgggtgagcg agcaggtcgg
    21361 gcagccggtg ctggtggaga accgcgccgg cgccggcggc acgatcgcgg ccgcggaagt
    21421 ggcgcgctcg gcgcccgacg gctacacgct cctcatccac aacatcacct tcccgatcgc
    21481 ctcggtcgcc gccgcgctcg ccaaccgcgc gacctacaac gcggacgcgg atttcgccgg
    21541 cgtctcgatc gcggtgtacg tgcctttcgt ctggaccgcg aatcctgcga cgcccggcaa
    21601 ggacctgcgc gacgcgatga gtcatgtaaa gcagttcaag ccctacacgt acggctccac
    21661 cggaccgggc tccacgatgc acgtgctcgg cgaagcgctg tcgcgcgccg ccggcgtcga
    21721 gatgacgcac atccctttca agggcgccgc gccgctcaag caggagctga tcgccgggcg
    21781 catccacctc ggcggcgacc agctctcgag ctcgctcgcc gacatcaagg ccgggaagct
    21841 caaggcgctc gccaccggcg cttcgcggcg catcgccgag ctgcccgacg tgccgacggt
    21901 gcgcgagctc ggctacccga tgctcgaggc cgagggctgg aacggctttt tcgccccggc
    21961 gaagaccccg cgcgaggtgc tcgagcgcct gaatcgggag ttcgcgaccg cggtcaagca
    22021 tcccgacgtg gccaagcgca tgcgcgagat ggcggccgag ccggtcggct cctcgggcgc
    22081 cgagcaggag gcgatcttca agcggcagat ggaccagttc cggcccatca tcaaggaaat
    22141 gcgaatagag gggtaactat gagaaagatc tttctcggat tgcttttcgt ttgcggaacc
    22201 gcgattgctc agacgttccc gacgaaaccg gtgaaactca tcgtcaccta tccgcccggc
    22261 ggcagctcgg acctgatgtc gcgcgtctac ggcgcgaagc tcggcgacct gtggggccag
    22321 caggtgatca tcgaatcgaa gcccggcgcc gccggctcga tcggcatgga cttcgcggcg
    22381 aagcagccga acgacggcta caccttcgtg gtcggcaacc tcggcccggt ggcagtgaac
    22441 ccgctgctct ccaaggtgcc gtacaacatc gagaaggact tcatcccggt atcgatgatt
    22501 tccaccgggc cgaacgtcct ggtggtgcgc accgattcgc agttcaagtc gctggccgag
    22561 atcatcgact acgcgaagaa gaaccccggc aagctcaact acggcaccag cggcccgggc
    22621 agcgtgtcgc acctctcgag cgagatgctg aagaacggcc agaaggtcga ggcggtcgaa
    22681 gtggcctaca agggcggggt gctggcgatc caggacctgc tcggcggcca gatccagtac
    22741 atcttctccg actcgctgcc ggcgatgggg catatcaagg ccggcaagct gcgtgcgctc
    22801 tgcgtcaccg gcgagagccg ctacgcgctc ctgcccgagc cgccgctgtg ccgcgaagcg
    22861 gcgccgggcc tggtggcggt caactggtgg ggcgtgctgc tgcccgccgg cagcccgaag
    22921 agcgtagtcg acaagctgaa cgccgacacg gtcaaggcgc tggccgatcc cgacgtcaag
    22981 aagcgcttcg ccgacctggg cgtggaagcg gtgtccagca cgccggcgga attcgccgcg
    23041 ttcatcaaag ccgagatgga caagtacgcg aagctgatca aggaagcgaa tatcaaagtg
    23101 aatccatgaa gctttacggc ttctggcggt cgttggcgag ctaccgcgtg cgcgtggcgc
    23161 tcgccctgaa gggcctgcag gccgaggaga tctcgatcga tctcctgaag ggccggcagt
    23221 cgagcccgga ctacctcgcg gtcaacccgc agggcgtggt gcctgcgctc atattggatg
    23281 acggcgggcc gccgctgttc cagtcgatcg cgatcctcga gtacctggag gaaatacgcc
    23341 ccgtgccgcc gctgcttccc agggacccgc gcggccgcgc gcgcgtgcgc gggcttgcgc
    23401 tgatcgccgc ggcggacggg catccgctgg tcacgccgag aatccgcaac tacctggaaa
    23461 aggaaatgcg ccaggacgag gccgcgcgca accgctggct cggccactgg accatgcacg
    23521 cgctcgaggc gatcgaaggc cacctgtcga gggagaagga gaccggccgg ttctgccacg
    23581 gcaacacccc gacgatggcc gacatctgcc tcgccgggca ggtgatcggc gcgctcgcgt
    23641 acttcagctg cgacaccagc tcgatacccg cggcgatgcg catctacaac cagtgcatgg
    23701 agatcgaagc cttcgccaag gcccatcctc tgaagcaacc gggagcaaga cctcactaat
    23761 gaaacccatc gaagtcgccg tcatcggcac cggctggtgc ggcggaatcc gcgccgagac
    23821 tctcgccgcg catccgctgg tcaaagcgct gcacctctct gagatcaaca aggttcgcct
    23881 gcaggaaatc aaggcgaaga ccaagccgaa gaccgcgggc accgattaca aggagttatt
    23941 gaagaaagcc gagatcgaag cggtctacat ctccgccacg ccggagacta cgcattaccc
    24001 gatcgcgcgc gactgcttgg ctgccggcaa gcacgtgttc ctcgagaagc cgatcgcgct
    24061 gacgctggaa gaggcggacg aactgattgg tcttgcaaaa aaatcccgtc tcaaatttac
    24121 gatcggctac tctcagcgct tcaacccgaa gttcgcctac gtgcgccagt gcatccgcga
    24181 tggcaccatc ggcaggccgg tgagcgcgct ggtgagccgg cacatcaccc ggagcctcgg
    24241 caagaagatc agcggccggg tcaagctctc gcccgcggcg atggagtcga cgcacgacct
    24301 cgacttcctg ctctggtgcc tcgagcccgc gaaaccggtg cgcgtctact cgcagcagaa
    24361 cttcggcgtg atgcaggaga ccagcggcca gccgatcgcg gacacgcagt ggatcaccgt
    24421 cacgctcgac agcgggctca ccttcgtcgt cggcggcggc tggaccctgc cgctcggcta
    24481 cccgaacttc tccaccacct ggatcgagat ggtcggcacc gagggcgccg tgctcgtgga
    24541 cgactcgcac cgggatgtac tggtgaacac ggtgaagaag ggcatgcagc ttccgatgtc
    24601 cacgatgccg ggcgagttcg tcgagcacac ctacgccggc gcgatggcgc ccgagacggt
    24661 gcacttcctc gaggcggtgg tctacgaccg gccggtgctg gtgacgcccg agcacgcgcg
    24721 catggtgatg gaggtctaca tcgcggccga cctgtcggcc gagcgcaacg ccgccgtgga
    24781 tcttccatgc gaaaaaccgc gaagcggatc ggcctcgcga tcgtcggcgc gggccgcgta
    24841 ggcctgttcc gcggcgaggt cgccgcgcgc cacccgatgg tcgacttcat cggcatcgcc
    24901 gacctgaagg aggaccggct cgagctcgtc cagaagaaga cgcaggccga tttcgccacc
    24961 accgatttca gggaattgct caagagacct gaaattactg cggcgatcat ttcgaccgac
    25021 gagcacctgc acgtggagcc gatcctcgcc gcctgcgagc gcggcatcag cctgctgatc
    25081 gagaagccgc tcgccacgga gctcgccgat tctcaaaagg tattggattt cattacgaag
    25141 aagaaagtcg atgccgtcgt cggctatacc cagcgcttcc gccggcgctg gctcgcggcg
    25201 aaggagaagg tgcgcaccgg cagcctcggc gacgtcacgc tggtcagctc gcgcgccttc
    25261 atgaaccggc tggtcgcgct cgacaactac aagcgcacct cggacgcgtc gaagatctcg
    25321 ccgatggtca tttccgggac gcacgcgctc gacatcgtga tgtggatgat ggaggcgaag
    25381 aagccggtcg agctctacgc ccgctcggtc gacaaggcgc tcgggccgct gtgcaaggga
    25441 atcgacgcca ccgccgggac cctcgttttc gaggacggca gcctgtatca cgcctgcatc
    25501 tcgtgggcgc tgccgatcgt gtggccgggc gcggtgtaca gcctggaggt cgggatcacc
    25561 ggcaccgagg gcgtgctcac catcgacgac acgcatcgcg acatcgttct tgccacgacg
    25621 aaagcccaat ccgagggcta tgcgccagac tctcagcgca gggtcgactt cctgggaagc
    25681 tatccgccgg gcgacatggc gctcggcgag ctgcgcggcc cgatgcgcga ggagaccgag
    25741 cagtggctga accgcgtctc gctcggcctg tccacccagc atgcgaccgc cgccgaggcg
    25801 cacaaccgcc tgatgcttac caaggcgttc gacctttcgg cgcggcgcaa aaagcctgtg
    25861 caacttccaa taacggagga ttcatgaaaa agctgctttc cctgttggtg gcgttggtgg
    25921 tgcccacgat tgcctggggt cagtacccca acaaggccat acgcatgatc gtgccgttcg
    25981 cgccgggcgg cgcctcggac ttcgtcggcc gcatcctgca gccgcgcctg accgagctcc
    26041 tcggccagca aatcgtggtc gacaaccgcg ccggcgcctc gggcaacatc ggcatggacg
    26101 cggcggcgaa gtcggcgccc gatggctaca cgatctacct cggcaacgtc ggcacggtcg
    26161 cgctcaaccc ggcggtgttc ccgaagctcg gggtcacccc gaccaaggac ttcgtggcca
    26221 tcacccaggt ggtggacgtg cccggcgtgc tggtggtgaa cccggagctg caggcgaaga
    26281 cggtgaagga gatcgtcgcc atcgccaagg cctatcccgg caagctcaac tacgcctcgc
    26341 cgggcgccgg cagccagaac cggctcgaga tggaagtgtt ccgcaaggtc gccggcgggc
    26401 tcgacatggt gcacgttccc tacaaaggcg gcgccggacc cgcggttacg ggcctggtcg
    26461 gcggcgagac gcacctcatg ttcgtcaccg cgtcctcggc gatgaaccac gtgaagagcg
    26521 gccgaatccg cctgatcgcc gtaaccagct ccaagcgcct cgaggcgttc ccggacgcgc
    26581 ccaccatggc ggagtccggc ttccccgagc tcaccagcgg ctcctggcag ggcatcttcg
    26641 ttcccacggg gacgccgaag gaagtcgtcg acaagctcta cggcgcgatc atggaaacca
    26701 tgaagacgcc ggaggtgaag cagcgactat cgaacggcgg cgtcgaggtc gtcaccagcg
    26761 cgccgggcga gttcgcgcag ttcgtcgcca aggagaccga gcgctggggc aaggcggtga
    26821 aggaagcggg cgccaccgcc gagtagaaaa aaccaacgaa ccactggaga gaagagcaat
    26881 ggctaacgca ggaatggtgg cactggactg gcagcagcgc atcaattggg accgggtgcg
    26941 caagtaccgc ctcgagcgcg cgcgagagaa gatgaaggcg cacgggctgg gcgcgatgct
    27001 gctcatgtac gacgagaacg tgcgctacgt gaccagcacg ctgacgccgg gctggaaccg
    27061 gctcaagccc ggcctgcgct acgccatgct gtgcggcgac gacgcgccgg tgctgttcga
    27121 gcagggcgac atcggcttcc agatccagcg ccactcgccg tggatcccga aagaccacgt
    27181 gcgctactcc tacgcctgga tcaagggcgc cgccggcccc gcttccagca tgcaggtgaa
    27241 gaagttcacc aacgcggtca agcaggagat gaagaaggcg aacgtcgcgg gcgcgaagct
    27301 cggcgtcgac ttcatcgaca tcaacatgct gaaggtgttc gccgacgaga agatcgactg
    27361 gaccgacggc atgacgccga tgatggaggc gcgcgccgtc aagaacgagg acgagcagga
    27421 agcgctgcgc atggtcggcg cgatcggcga cgcggcgcac tgggagttca tgagctacct
    27481 caggcccggg cgcaccgaga accagctcac cgcgcacatc atggagtacc tgtactcggt
    27541 gccggggatg gaggacgtcg aggacgtgat cgtgtcctcg gggccgaaca cctggcccaa
    27601 ctggcgcaac ttctccgacc gcatcgtcaa gcccggcgac atcgtgttca tggacctcgc
    27661 cgcgctcacc tggaacggct acaagtcctg ctactaccgc acctactgcg cgggcaagga
    27721 gccgagcaag gagcagaagg acatctacgc cactgcgctg aaatggctgt acgactcgat
    27781 caaggcggtg agggtcggcg cgaccaccaa ggacatcgcc tcgaagtggc cttcggccaa
    27841 ggaagcctgg ggctacgagg aggaagacca ggccgcggcg aacctgtggg gccacgggct
    27901 ggggctggcg cagtacgacc agccggtgat ctcgcgcatc tggtcgctcg accacccgat
    27961 cgagatcaag gaaggcatgg tgttcgcgct cgagacccag cacggcaagc gcttcgagtg
    28021 gggcgtgcgc atcgaagaga tgctgatcgt gcacaaggac aagatcgaga tcatctccaa
    28081 cttcccggtc gagcagatca cggtggtcga cccgatcacg ggctattcca cgctcgcggg
    28141 ccggtgatta gttaatgcgg accgcgcttc tccgagcccc tcggaagctc gacctcgtgg
    28201 ttcgcgagaa gccgcggccc gggccgggcg aggtgctggt gcgcaccgct gcgaccgcgg
    28261 tgtgccacac cgacctttcc atttacaccg gcgcgcaccc cggggtgcgc tacccggtgg
    28321 tgatggggca cgagtcgacc ggcacggtgg acgctgtggg agaaggcgta gggcttcgta
    28381 ttggaagccc cgtgatcatc aatcccatta tcgcctgcgg ccattgcgat ccgtgccggc
    28441 gcggcgcggg caacctgtgc cgcaacgccg gcctcttcgg ccgtgaggtc gagggctcgc
    28501 tcagcgagta tgtgaagctg ccgtcgcaat acatctatga gctccccgcg ggactttcgc
    28561 tagagaaggc gaccctgatc gagacgctcg ccacggtgcg ccacgcgcag caccgtgccg
    28621 gcctcgcgaa gggcgaatcg gtggtggtgc tggggcaggg caccggcggc ctgttgcaca
    28681 ctcggctcgc ggtgctgacc ggcgccagcc cggtcatcgc ggtgtcgcgc acccgctgga
    28741 agctggagat ggccgagaag atgggcgcgc agcacgtggt cgcggcctcc gcgatggatg
    28801 cagtcgaaga ggtcaagcgc ctgaccggcg gcaagggcgc cgacctggtg atcgacgctg
    28861 ccggcggcgc cgaggcgctg aaagcagcgc tcgccatgct gcgccccggc gggcgcttct
    28921 ccgcctactc gatcagccac gagccggtgc acggcttctc caccttcccg ctctatttcg
    28981 gcgagatcag cctgatcggc tcgcgcgcgc tcaccgctgc ggacatggcg ccgagcatcg
    29041 agctcgtcgc ctccggcaaa gttgacgtcg acggctttgt cagcgccaag tacccgctcg
    29101 ccgataccgc cgaggcattc aaggaatacg aaggcaaccc cggcaggatc ctgcgcatcg
    29161 tgatcgactc gcaaaaatga aaccctggac cccgaaatca gggacagacc acgtttttcc
    29221 ggactggcca gctggtggac gaaaacgtgg tctgtccctg attaggtcgg ttcaatgacc
    29281 ttcgtccttc ctctgaaaga cagtcaggcg gcgaacgccg cccgcttcgg ttccaaagct
    29341 gcgaggctcg ccgcgctcag ccacgctggg ctgcctacgc ccggaggctt ctgcgtggat
    29401 gcgcaagcct accgcctgca gctcgcccac ctcggcctcg aggaggcggc gcgcggcgtg
    29461 ttcgcggccg aagaccggcc gcaggcgcgg cggcatgcgc tttcggtcaa gctggggttg
    29521 atgaccgggg agatttttcc ggccctccgc gacgatctgc tcgagatctt caacccggta
    29581 gtgcgctcct cggcgctggt ggaagacaag gccggtgcga gttttgccgg ccagttccag
    29641 agctacctcg ggctcgagac ggaggcggac ttcctgaccg cggtgcgcgc ctgctgggct
    29701 gcactctggt ccacgcgcgc gctgcggtat ttcgcagctt ctggcctcga ccccgctgca
    29761 accggaatgg ggctgatcat ccagccgctc gtgcaggcgc gctgcgcggg cgggggcctg
    29821 tcgcgcaccg ccgacggcga catgctggtg aacgccgccg cgggccccgg ctcgtcggtg
    29881 gcgcaaggcg aggtggtgcc cgaccgctac gtgctcgatc gctccggcgc gctgaaggaa
    29941 tccacggtcg gaacgaacta tcacagcatg ggctgcaagc acgggcgccg cgcgttttcg
    30001 cgcttgctgg cggcgccgcg cctcgatgcg gagcagctcg ccgaactggg ttcattcatg
    30061 aagaaagcgg aagaactgat gcagggcccg gtcgagatcg agtgggcgct ggacgacgcg
    30121 ggcatcaagc tcctgcaggc gcggccgctg agcgtggccg aagcgccggt gccggacgag
    30181 atctggctga agcatccgcg gctttcgggc catcccggcg gcgcgggctg gggcacggga
    30241 cgcgcgcgga tcgtgagctg cgagtgcgag ctcgagcgcg tggcgccggg cgacgtgctg
    30301 gtcacgaccg tcgccggtcc cgccctgtcg gacgtatttc atcgcgttgc cggcatcgtg
    30361 gccgagctcg gcggcagcac ctctcacctc gcgtcgctcg cccgcgagcg cggcatcccg
    30421 atggtgctgg gcgcgatgga cgccacccgc cgaatccccg acggcgcgat ggtggcggtc
    30481 gacggggtgg caggggtggt gcgctggatg agggcctgat ggcaaaagcg aaagtcgtgc
    30541 tcaccgatta cgtctgggaa tcgctcgacg tggagaagaa gacgctcgag ggcctcgcgg
    30601 acctggtggc gctcaagacc aagaagcccg aggagttcct cggcgaggcc gcggactgcg
    30661 acgcgctcct caacacctac gccgggccga tcaccgcgca ggacatggcg cgcatgccca
    30721 aatgccggat catcgcccgc tacggcatcg gcgtggacac catcgacctg gaggcggcga
    30781 ccgccgccgg cattatcgtg accaataatc ccacctactg catcgaggaa gtggccgagc
    30841 agaccatggc gatgctgctc gcctgcgcgc gcaagatcgc gttttacgac cgcatggtgc
    30901 gcgccggccg gtgggaagtg ccgccgggca agccgatgtt ccgcctcgtc gggcagacgc
    30961 tcggcctggt gggcttcggc aacatcgcgc gccaggttgc gatccgcgcc gcggcgttcg
    31021 gcatgcgtgt gctcttctcc gatccgttcg tgaaagaggg acctggaaaa acagacctgg
    31081 aaaagctcct cgcggagtcg gacttcgtgt cgctgcaccc gccgctcacg ccgcagacgc
    31141 gcaagatgat caacgatgag gcgttcgcga aaatgaagcc gaccgcgttc ctcatcaatt
    31201 gctcgcgcgg gccggtggtc gacaccgagg cgctggtgcg cgcgctcgac gcgaagcgca
    31261 tcgcgggcgc cgccctcgat accgtcgatc ccgagccgct gcccaacccg catccgctgc
    31321 gcgggcgcga gaacgtcatc ctctgcccgc acgccgcctg gtacagcgag caggcgatgc
    31381 gcggcctgca ggcgggcgcg ccgggcgaag tgcgccgcgt gctgaccggc gagtggccgg
    31441 tcaacgt
//
LOCUS       DATBSZ010000061        12781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2056876, whole genome shotgun sequence.
ACCESSION   DATBSZ010000061 DATBSZ010000000
VERSION     DATBSZ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12781
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2056876"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..801
                     /locus_tag="VIG70_04070"
     CDS             37..801
                     /locus_tag="VIG70_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY8249759.1"
                     /translation="MSVHPLKSPAADPVQEAKVHLAAAHRLAVLHELEEGIDNHFTVT
                     VPGHNDRYLILPFGLHWSEARARDLIVFDESGATLEGEGVVELSAQCIHAPIHRICGA
                     RVVMHTHQTWALALNMLKDNRLVPASQTAAFFHGHVAYDDTYAGTADVLEEGERLAHV
                     IGSKHVLFMKNHGVLVTGATVAQAYRRLYKLERVCRAQVLAMSTGKPLEILSEDIVAQ
                     VQAPPANDRHSRVERERLFFEAMMRILDREMPGYAS"
     gene            874..1245
                     /locus_tag="VIG70_04075"
     CDS             874..1245
                     /locus_tag="VIG70_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8249760.1"
                     /translation="MRILVLDDNADLLESMRLLLASCGHEAVTSTDAREAIDIQSRRP
                     ADVLITDIFMPGTDGLEAIVAFRSRWPDLKIVAISGGGVVAKRDYLGVAAEIGANAMM
                     RKPFDPQELLDTVDRFARGEG"
     gene            1283..2539
                     /locus_tag="VIG70_04080"
     CDS             1283..2539
                     /locus_tag="VIG70_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY8249761.1"
                     /translation="MTWIIVVLLIAVLVLGLAIWSIKRHKDPHLALESREPLDKLIPS
                     ISGLSLGMAIRGNAVEIFQNGAFFDALLEDILAARHTINFETFLWKEGRIGQRTADAL
                     SKQARAGVKVRLVLDAVGCKEIGEGARAQMRDAGCELAIYHPRTLKNIGVVAERDHRK
                     LTILDGHTAWVGGHCIVDEWLGEAQDRHHVRDLSVRLRGPVVHAVQSVFSENWVEVTG
                     RLFVGDEYFPPLQPAGEVEAHIASMKPEGSAPAVKILHHAVICCARERLWIQNPYFIP
                     EPDAIDAFAQAVERGVDVRIMVPSTEVSDMPIVQHAAHRNFQQLLDSGVRIFEYRRTL
                     LHQKVMTVDGVWCAVGSSNFDDRSFETNDEITVGFHDRALARRLEEIFEEDRVHCAEL
                     QAAQWKKRGLLHRAKDSALYLFNEVL"
     gene            complement(2540..3499)
                     /locus_tag="VIG70_04085"
     CDS             complement(2540..3499)
                     /locus_tag="VIG70_04085"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249762.1"
                     /translation="MLMLRLLVAICALSAAHALAQGWPARPVRFVVPFPPGGSTDVAA
                     RTLADKLTRSLGAQFVVENRAGGSGAIGTTAVARAAPDGYTILFAADPVTTLFLVVKN
                     LEFDTLRDFAAITQVTTQPLAVAVHASVPATSVQELVAYAKANPGKLSFAHSGTGTGQ
                     HFSGELLKKMAGLDIVHVPYKGGGPAVQDLVAGQVPMGVLGSTPLIPHHKSGRIRILA
                     FTSKDRFATMPEIATLHESGFPGFDTGQWLGLLAPRGTPADIVQRLYAATAEALSLAD
                     VRERLAQAALQPVGNTPREFEAVIRADIERWGKLAQELGIQPQ"
     gene            3557..4990
                     /locus_tag="VIG70_04090"
     CDS             3557..4990
                     /locus_tag="VIG70_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEY8249763.1"
                     /translation="MLRLVFAFVVLLPCAAMAQAMGAADARHLLNRAGFDARLEQVDE
                     FAKLARGAAVERLLAGAGGAARTPAPAWVKEWTDPRRLRQMDAEERKLFVREQVERGL
                     ELKGWWFTEMAATASPLTERMTLFWHNHFTSSLQKVRSPPLLYRQNVLLRKHALGNFG
                     ELLHAVSRDPAMLVYLDAATNRRGQPNENFAREVMELFTLGEGRYGERDIREAARAFT
                     GWSIDLPSGEYLYRPRLHDGGEKTVLGRSGNLRGEDVLDILLAQPATAEFVAAKLWRE
                     FVSPQAAPAELARVAAVFRASGYDIRSALRALFATEAFWATENRAALIKSPAELVVGT
                     LRQFDIGFVDPLPFVLLLRALGQDIFSPPNVKGWPGGEAWINSTTLLARKQFLDRVFR
                     VEETRIAMQRPAMREKLGTLKPGARRFMQAVADLQFSARDWQRPFEGREKMVPVVLLA
                     AAPAGSPSGEGMDYVRALVADPVYQLK"
     gene            5049..6134
                     /locus_tag="VIG70_04095"
     CDS             5049..6134
                     /locus_tag="VIG70_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY8249764.1"
                     /translation="MFAQSSGYRRLLVLIELKGGNDGLNTVVPYDDDAYYRLRPRIGV
                     RRDDVLQLDGRTGLHPALKPLLPLWQARELAIVQGVGYPLPNLSHFRSIEIWDTASKS
                     DEYLTEGWLARAFAGQPVPAQFAADAIAVGGNDMGPLAGSGTRAIALNDPAAFLRQAR
                     LANPLGKSHNVALNHILKVEADIAKAASQLHGGMQFGTEFPQGPFGNAVRVAAQLAAN
                     PAGVAAIRLSLGGFDTHQNQPGVHQRLLGMLAEGIAALKSALLEASRWDSTLVMTYAE
                     FGRRPQENNSNGTDHGTASAHFLTGGRVKGGLYGDAPRLSDLDGGNLRHAVDFRSLYA
                     TGLERWWGLSSAGVLKGRFAPLDVLRA"
     gene            complement(6136..6909)
                     /locus_tag="VIG70_04100"
     CDS             complement(6136..6909)
                     /locus_tag="VIG70_04100"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY8249765.1"
                     /translation="MHPMLNIAVRAARRAGSIINRAALDGGGYEVRSKRMNDFVTKVD
                     HAAEESIIEIVRKAYPDHAVLAEESGASTERSEYQWVIDPLDGTTNFIHGFPQYCVSI
                     AIRHRSALAHGVIYDPVKNELFTASKGRGAFLNDRRIRVSKCLRLGDALIGTGFPFKE
                     LGQIERYTGQLKQIMQKTAGVRRAGAAALDLAYVACGRLDAFWELGLSAWDMAAGALM
                     IQEAGGLVGDLRGEDGYLESGEIACATPKIFPPLLEILK"
     gene            6945..7634
                     /locus_tag="VIG70_04105"
     CDS             6945..7634
                     /locus_tag="VIG70_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY8249766.1"
                     /translation="MREIRFVLCRPSHPGNIGAAARAMKTMGLSDLRLVAPERFPAKE
                     AQWMATHAGDVLENATVHENLLDSIKDCVASFALSARGREWSPQVLDVRTAAAQATQI
                     DGPVAFVFGNETAGLTNDEMFACQHLVHIPASREFRSLNLAQAVQVVAYEVRMCLDVA
                     IPFSRSEKLATVEDLEGLYAHLEEAAVASGFMAPASRLRERWRRLFSRVPALEREEVN
                     ILRGLLKALLK"
     gene            7659..8366
                     /gene="cysE"
                     /locus_tag="VIG70_04110"
     CDS             7659..8366
                     /gene="cysE"
                     /locus_tag="VIG70_04110"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEY8249767.1"
                     /translation="MFRKLRADLDCILERDPAARSRWDVLTCYPGLHAVYVHKLAHWF
                     WLRRLYWLGRFTSHLGRWLTGIEIHPGARIGCCVFIDHGMGVVIGETAEVGDGCTIYQ
                     GVTLGGTSLEPGTKRHPTLGAGVIVGAGAKVLGGFKVGDGARIGSNAIVVKEVPAGAT
                     VVGMPGRMLEKAGTREAERFAAYAVVQEQDDPYAKAIQQLVEHSQELERAVAALNEKL
                     ARLENADREHPLRVVQK"
     gene            8481..8942
                     /gene="iscR"
                     /locus_tag="VIG70_04115"
     CDS             8481..8942
                     /gene="iscR"
                     /locus_tag="VIG70_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEY8249768.1"
                     /translation="MRLTTKGRFAVTAMVDLALRQNRGPVTLAAISERQHISLSYLEQ
                     LFGKLRRASLVSSVRGPGGGYNLAQPPNGISVADIVAAVDEPLDATQCGGKENCHDDK
                     RCMTHDLWATLNEKMYEYLSSVSLADLVAHQSGKPVSVIKDFRPSSEKVAA"
     gene            8939..10147
                     /locus_tag="VIG70_04120"
     CDS             8939..10147
                     /locus_tag="VIG70_04120"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEY8249769.1"
                     /translation="MRLPVYLDYSATTPVDPRVAGKMIPYLTEFFGNAASRSHAFGWK
                     AEEAVEEARGHVAALINADPKEIVWTSGATEGNNLAIKGAANFYKTKGKHIITQKTEH
                     KAVLDTCRELERHGFEVTYLEVDSNGLVSVERVKAALRPDTILVSIMMVNNEIGVIQP
                     VWEIGELCRAKGIIFHCDAVQGGGRLDIDVQKFKVDLLTITAHKIYGPKGIGALYVRR
                     KPRVRIEAQIHGGGHERGFRSGTLATHQIVGFGEAARLAKAEMASENERIRALRDRLY
                     KGIQGIEEAYVNGDLKQRIPHNLNVSFNFVEGESLIMAVKDLAVSSGSACTSASLEPS
                     YVLRALGRSDELAHSSIRFTLGRFTTEEEIDFAIELIKRKVAKLRELSPLWEMHQEGI
                     DLNTVQWAAH"
     gene            10186..10596
                     /gene="iscU"
                     /locus_tag="VIG70_04125"
     CDS             10186..10596
                     /gene="iscU"
                     /locus_tag="VIG70_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEY8249770.1"
                     /translation="MAYSDKVIDHYENPRNVGSFDKADDAVGTGMVGAPACGDVMKLQ
                     IKVGADGRIEDARFKTYGCGSAIASSSLVTEWVKGKTLEQATTIKNTQIAQELSLPPV
                     KIHCSILAEDAIKAAVADYRKKHEVSESKDKKVA"
     gene            10596..10922
                     /gene="iscA"
                     /locus_tag="VIG70_04130"
     CDS             10596..10922
                     /gene="iscA"
                     /locus_tag="VIG70_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEY8249771.1"
                     /translation="MAITLTPNAAGHVQRSLSKRGKGLGLRVGVRTSGCSGLAYKLEF
                     ADQANPGDLEFESHGVKVLVDPKSLQYIDGTELDYTREGLNEGFKFRNPKVKDECGCG
                     ESFNVA"
     gene            10994..11500
                     /gene="hscB"
                     /locus_tag="VIG70_04135"
     CDS             10994..11500
                     /gene="hscB"
                     /locus_tag="VIG70_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEY8249772.1"
                     /translation="MQNHFDLFGLAPAFAVEAEALERSYREIQSRVHPDRFAHAGDAE
                     RRASLQWTTRVNEAFQILKNPVTRAKHLLELHGVDVAFETNTAMPADFLVQQMELRET
                     LENARDAAALDALRDSLGKDKRALEKQIGEAIDRRKDYAGAAGLVRKLQFLDRLDTEI
                     DSAYEGVE"
     gene            11501..>12781
                     /gene="hscA"
                     /locus_tag="VIG70_04140"
     CDS             11501..>12781
                     /gene="hscA"
                     /locus_tag="VIG70_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEY8249773.1"
                     /translation="MALMQISEPGMSPEPHQARLAVGIDLGTTNSLVATVRSGVPAVL
                     PDAHGRPLLPSIVRYFPDGRAEVGYAAQARQAEDPKNTVVSVKRYMGRGVKDVAHIEN
                     APYDFVDAPGMLQVRTVAGVKSPVEVSADILRVLRERAELSLQGNIFGAVITVPAYFD
                     DAQRQATKDAGRIAGLNVLRLLNEPTAAAIAYGLEHAAEGTYAVYDLGGGTFDISILK
                     LSKEVFEVVATSGDSMLGGDDFDQRVFCWAVEQAKLPPLDPRDMRRLLVKAREAKEHL
                     TVHGEAPITAKLSTGEVVDLSLDTETFCSITQTLVQKTLGPVRKALRDAGLRVEDVKG
                     VVMVGGATRMPQIQRAVGEFFRRDPLNNLDPDKVVALGAAMQANVLAGNKPQGEDWLL
                     LDVIPLSLGIETMGGLTEKIIPRNTTIPAGRAQEF"
BASE COUNT         2207 a         4205 c         4331 g         2038 t
ORIGIN      
        1 gatgccgcga ccagcctagt cctgtagcat tcacccatgt ccgtccatcc cctgaaatcg
       61 ccggcagccg acccggtgca ggaagccaag gttcacctcg cggccgccca ccgcctggcg
      121 gtgctgcacg agctggaaga aggcatcgac aaccacttca cggtcactgt ccccggccac
      181 aacgaccgct acctgatcct gccgttcggc ctgcactggt ccgaggcccg cgcccgcgac
      241 ctcattgtct tcgacgagtc gggcgcgacc ctggaaggcg agggcgtggt ggagctgtcg
      301 gcgcagtgca tccacgcgcc gatccaccgc atctgcggcg cgcgggtggt catgcacacc
      361 caccagacct gggcgctggc gctcaacatg ctgaaggaca accgcctggt gccggcgagc
      421 cagaccgccg cgttcttcca cggccacgtc gcctacgacg acacgtacgc cggcacggcg
      481 gatgtcctcg aggaaggcga gcggctggcg cacgtgatcg gcagcaagca tgtcctgttc
      541 atgaagaacc acggcgtgct ggtgaccggc gccacggtgg cgcaggctta ccgccggctc
      601 tacaagctcg agcgcgtctg ccgcgcccag gtgcttgcaa tgtcgaccgg caagccactg
      661 gagattctgt ccgaggacat cgtcgcgcag gtgcaggcgc cgccggcaaa cgatcgccat
      721 tcgcgcgtcg agcgcgagcg gctgttcttc gaggcaatga tgcgcatcct ggaccgcgag
      781 atgccgggct acgccagcta agtagtttca ggttgtcggg cgacgcccga cagaccgcag
      841 ccaccgcgct ttggcaatat cgcatccaag aaaatgcgca tcctggtcct ggacgacaac
      901 gccgacctgc tcgagtcgat gaggctgctt ctcgcgagct gcggccacga ggccgtgacc
      961 tcgaccgatg cgcgcgaagc gatcgacatc cagagccggc ggccggccga cgtcctgatc
     1021 accgacatct tcatgccggg gaccgacggg ctggaggcga tcgttgcgtt tcgcagccgc
     1081 tggcccgatc tcaagatcgt cgccatctcg ggcggcggcg tggtcgcgaa gcgggactat
     1141 ctcggtgtgg cggccgagat cggcgcgaac gccatgatgc gcaagccgtt cgatccgcag
     1201 gagctgctcg acacggtgga ccggttcgca cgcggcgagg gttgagcaac gcttgtagtg
     1261 gttcaggcgt attttccccg ccatgacctg gatcatcgtg gtgttgctga tcgccgtcct
     1321 ggtgctcggc ctcgcgatct ggtcgatcaa gcggcacaag gatccccacc tcgcgctcga
     1381 atcgcgcgag ccgctggaca agctgatccc ctccatttcc gggctgtcgc tggggatggc
     1441 gatccgcggc aatgcggtgg agattttcca gaacggcgcg ttcttcgatg cgctgctgga
     1501 agacatcctg gcggcgcggc acacgatcaa ttttgaaacc ttcctctgga aagaaggccg
     1561 gatcgggcag cgcacggccg acgcgttgtc caagcaggcg cgcgccggcg tcaaggtgcg
     1621 cctggtgctg gacgcggtcg gatgcaagga gatcggggaa ggggcgcgcg cgcagatgcg
     1681 cgacgccggc tgcgagctcg cgatctacca cccgcgcacg ctgaagaaca tcggcgtggt
     1741 cgccgaacgc gaccaccgca agctcacgat cctcgatggc catacggcct gggtcggcgg
     1801 ccattgcatc gtcgacgaat ggctgggcga ggcgcaggac cggcatcacg tgcgcgacct
     1861 gtcggtacgc cttcgcggtc ccgtggtgca cgcggtgcag tcggtgttca gcgagaactg
     1921 ggtggaggtg accgggcggc tcttcgtcgg cgacgagtac tttcctccgc tgcagcccgc
     1981 gggggaggtc gaggcgcaca tcgcgagcat gaagcccgag ggctcggcgc cggcagtcaa
     2041 gatccttcac catgccgtca tttgctgcgc gcgcgagcgg ctctggatcc agaatccgta
     2101 cttcatcccc gagcccgacg cgatcgatgc tttcgcccag gcagtcgagc gcggcgtcga
     2161 cgttcgcatc atggtgccgt cgaccgaagt gtccgacatg ccgatcgtgc agcatgccgc
     2221 gcaccgcaac ttccagcagc ttctcgattc cggggtgcgg atcttcgagt accgcaggac
     2281 cttgctgcac cagaaagtca tgacggtcga cggcgtgtgg tgcgcggtcg gctcgagcaa
     2341 cttcgacgac cgctcgttcg agaccaacga cgagatcacc gtcggcttcc acgaccgggc
     2401 gctcgcccgg cggctggagg agatcttcga ggaggaccgc gttcactgcg ccgagctgca
     2461 ggcggcgcaa tggaaaaagc gcggcttgct gcaccgcgcg aaggacagcg cgctctatct
     2521 cttcaacgaa gtgctgtagc tactgcggct ggatgccgag ctcctgcgcg agcttgcccc
     2581 agcgctcgat gtcggcgcgg atcactgcct cgaattcgcg cggcgtgttg ccgacgggct
     2641 ggagcgccgc ctgcgccagg cgctcgcgta cgtcggcgag cgagagcgct tcagcggtcg
     2701 cggcgtacag ccgctggacg atgtccgccg gcgtgccgcg cggcgcgagc aggccgagcc
     2761 actggccggt atcgaacccg gggaagccgg actcgtgcag cgtggcgatc tccggcatgg
     2821 tcgcaaagcg atccttcgaa gtgaaggcga ggatgcggat gcgtcccgac ttgtggtgcg
     2881 gaatgagcgg ggtagagccg agcacgccca tcggcacctg gccggcgacc aggtcttgca
     2941 ccgccggccc gccgcctttg tagggaacgt gcacgatgtc gaggccggcc atttttttca
     3001 gcagctcgcc ggagaagtgc tggccggtgc cggtgccgga gtgggcgaag gagagcttgc
     3061 cgggattggc tttcgcgtag gcgacgagct cctgcacgct cgtcgccggg accgaggcgt
     3121 gcaccgccac cgcgagcggc tgcgtcgtca cctgcgtgat cgcggcgaag tcgcgcagcg
     3181 tatcgaactc caggttcttc accaccagga agagcgtggt caccgggtcg gcggcgaaca
     3241 ggatggtgta gccgtcgggc gcggcgcgcg cgacggcggt cgtgccgatg gcgccgctgc
     3301 cgccggcccg attctccacc acgaactgcg ctcccagcga gcgcgtcagc ttgtccgcga
     3361 gggtgcgcgc cgccacgtcg gtggatccgc ccggcgggaa cggcaccacg aaacgcaccg
     3421 gccgcgccgg ccagccctgc gcgagcgcgt gagcggccga cagcgcgcag atcgcgacca
     3481 gcaggcgaag catcagcatg cggagcctcc cgccgccatt gtcagcgaaa atggcggcgg
     3541 tgcgttagag gaggcgatgc tcaggctggt tttcgcattt gtagtcctcc tgccctgtgc
     3601 cgcgatggcg caggcaatgg gcgcggcgga cgcgcgccac ctcctcaacc gcgccggttt
     3661 cgacgcccgg ctggaacagg tggacgaatt cgccaagctc gcgcgcggcg cggcggtcga
     3721 gcggctgctc gccggcgccg gaggcgcggc gcgcacgccg gcgccggcat gggtcaagga
     3781 atggaccgac ccgcgacgcc tgcggcagat ggacgcggag gaacgcaagc tcttcgtgcg
     3841 cgagcaggtc gagcggggcc tggagctcaa gggctggtgg ttcaccgaga tggccgccac
     3901 cgcgtccccg ctcaccgagc gcatgacgct cttctggcac aaccatttca cctcgagcct
     3961 gcagaaggtg cgctcgccgc cgctcctcta tcgccaaaat gtgctgctgc gcaagcacgc
     4021 gctgggcaac ttcggcgagc tgctgcatgc cgtatccagg gaccccgcga tgctcgtcta
     4081 cctcgacgcg gccaccaacc gccgtggcca gccgaacgag aacttcgcgc gcgaggtgat
     4141 ggagctcttc accctcggcg agggccggta cggcgagcgc gacatccgcg aggcagcgcg
     4201 ggctttcacc ggctggagca tcgatctccc gagcggtgag tacctctacc ggccgaggct
     4261 gcacgacggc ggcgaaaaga cggtgctcgg gcgcagcggc aacctgcgcg gggaggacgt
     4321 gctcgacatc ctgcttgcgc agcccgccac cgccgagttc gtcgccgcca agctgtggcg
     4381 cgagttcgtg tcgccgcagg ccgcgcccgc cgagctcgca cgggtcgcgg ccgtgtttcg
     4441 cgcgagcggc tacgacatcc gcagcgcgct gcgcgcgctc tttgccaccg aggccttctg
     4501 ggccacggaa aaccgcgccg ccttgatcaa gtcgccggcg gagctcgtcg tcgggacgct
     4561 gcgccagttc gacatcggct tcgtcgaccc cctgccgttc gtgctgctgc tgcgcgcgct
     4621 ggggcaggac atcttctcgc cgccgaacgt caagggctgg ccgggcggcg aggcctggat
     4681 caactccacc acgctgctcg cgcgcaagca gttcctcgac cgcgtgttcc gcgtcgagga
     4741 aacgcgcatc gcgatgcaac gcccggcgat gcgcgaaaag ctcgggacgc tgaagcccgg
     4801 cgcgcgccgc ttcatgcagg cggtggccga cctgcagttc tcggcgcgcg actggcagcg
     4861 gccgttcgag ggacgcgaga agatggtgcc ggtcgtgctg ctcgctgcag cgccggccgg
     4921 cagcccttcg ggcgagggaa tggattacgt gcgcgcactc gtggccgatc ccgtctacca
     4981 gctgaaatag cgccgtggac cgcagaaact tcctcgccgc ctccgtcgcc gcgctttttt
     5041 caagctccct gtttgcgcag tcttcgggct accgccggct gctggtgctg atcgagctga
     5101 agggcggcaa cgacggtctg aacacggtgg tgccctatga cgacgacgcg tactaccggc
     5161 tgcggccgcg catcggcgtg cgccgcgacg acgtgctgca gctcgacggc cgcaccggcc
     5221 tgcaccccgc gctcaagccg ttgctcccgc tctggcaggc gcgcgagctc gcgatcgtgc
     5281 agggcgtggg atatccgctc ccgaacctgt cgcacttccg ctcgatcgag atctgggaca
     5341 ccgcctcgaa gagcgacgag tacctcaccg agggctggct cgcccgcgcc tttgccggcc
     5401 agccggtgcc ggcgcagttc gccgccgatg cgatcgcggt cggcggcaac gacatggggc
     5461 cgctcgcggg cagcggcacg cgcgccatcg cgctgaatga ccccgcggcg ttcctgcgcc
     5521 aggcgcggct cgcgaacccg ctcggcaagt cgcacaacgt cgcgctcaac cacatcctca
     5581 aggtcgaggc cgacatcgca aaggcggcga gccagcttca cggcggcatg cagttcggca
     5641 ccgagtttcc gcagggcccc ttcggcaacg cagtgcgcgt agcggcgcag ctcgccgcca
     5701 atcctgccgg cgtcgcggcg atccgcctgt cgctcggcgg tttcgacacc catcagaacc
     5761 agcccggcgt ccaccagcgg ctgctcggca tgctcgccga aggaatcgcc gcgctcaaga
     5821 gcgcgcttct ggaagcgagc cgctgggact cgacgctcgt gatgacctac gccgagttcg
     5881 gccgccggcc gcaggagaac aactccaacg gcaccgacca tggcaccgca agcgcgcatt
     5941 tcctcaccgg cggccgcgtc aagggtggcc tgtacggcga cgcgccgcgc ctctccgatc
     6001 tcgacggcgg caacctgcgc cacgcggtgg atttccgcag cctttacgcc accgggctcg
     6061 agcgctggtg gggcctgtcg agcgcgggcg tgctgaaggg ccgcttcgct ccgctcgacg
     6121 tgctgcgcgc ctgagctact tcaggatttc cagcagtggc gggaagattt tcggcgtcgc
     6181 gcaggcgatc tcgcccgact cgagatagcc gtcctcgccg cgcaggtcgc cgaccagccc
     6241 gccggcttcc tggatcatga gcgctccggc tgccatgtcc caggccgaca gcccgagctc
     6301 ccagaacgcg tcgagcctgc cgcatgcgac atacgcgaga tcgagcgcgg cggcgccggc
     6361 gcggcgcacc ccggcggtct tctgcatgat ctgcttgagc tggcccgtgt agcgctcgat
     6421 ctgcccgagc tccttgaacg gaaaaccggt gccgatgagc gcgtcgccca gccgcaggca
     6481 cttcgagacg cggatgcgcc ggtcgttgag gaaagcgccg cggcctttcg aggcggtgaa
     6541 cagctcgttc ttgaccggat cgtagatcac gccgtgcgcc agcgcgctgc ggtggcggat
     6601 ggcgatcgag acgcagtact gcgggaagcc gtggatgaag tttgtcgtgc cgtcgagggg
     6661 gtcgatgacc cactgatatt cgctgcgctc ggtgctggcg cccgattcct ccgccagcac
     6721 ggcatggtcg gggtaggcct tgcgcacgat ctcgatgatc gattcctcgg ccgcgtggtc
     6781 gaccttggtc acgaagtcgt tcatgcgttt cgagcgcacc tcgtagccgc cgccgtcgag
     6841 cgcggcgcgg ttgatgatcg acccggcgcg gcgcgcggca cgcaccgcga tgttgagcat
     6901 gggatgcatc ggggcgcgta ttttactgac ggtagacttc ggcaatgcgg gaaattcgct
     6961 ttgtcctgtg ccgtccgagc cacccgggaa atatcggcgc ggcggcgcgt gcgatgaaga
     7021 cgatggggct ttccgatctg cgcctggtcg caccggagcg atttcccgcc aaggaggcgc
     7081 aatggatggc gacccacgcc ggggacgttc tggaaaatgc gaccgtccat gagaatcttc
     7141 tcgattcgat caaagactgc gttgcgagct tcgccctgtc ggcccgcggg cgcgagtggt
     7201 cgccgcaggt gctggacgtg cgcacggcgg ccgcgcaggc cacgcagatc gacggcccgg
     7261 tcgcgttcgt cttcggcaat gagaccgccg gcctgaccaa cgacgagatg ttcgcctgcc
     7321 agcacctcgt tcacatcccg gccagccggg agttcaggtc gctcaacctc gcccaggcgg
     7381 tgcaggtagt ggcctacgag gtacgcatgt gtctcgacgt ggcgatccct ttttcccgct
     7441 cggagaagct cgccacggtt gaagacctcg aaggactcta tgcacacttg gaagaagcgg
     7501 cggtggcgag cgggttcatg gcgccggcgt cgcggctgcg cgagcgctgg cggcggctct
     7561 tttcgcgcgt gccggcgctc gaacgcgagg aggtgaacat cctgcggggc ctgctgaaag
     7621 ccttactaaa atagtcggaa acccatagaa tcgtgtttat gttcaggaaa ctgcgagcgg
     7681 acctcgactg catccttgag cgcgatccgg cggcgcgttc gcggtgggac gtgctgacct
     7741 gctacccggg gctgcacgcc gtctacgtcc acaagctcgc gcactggttc tggctgcggc
     7801 gtctttactg gctcggccgc tttacctcgc acctcgggcg ctggctgacc gggatcgaga
     7861 tccatcccgg cgccaggatc ggctgctgcg tcttcatcga ccacggcatg ggtgtcgtga
     7921 tcggcgaaac cgccgaggtc ggcgacggct gcaccatcta ccagggcgtg acgctcggcg
     7981 gtacttcgct cgagcccggg accaagcggc acccgacgct cggggcgggg gtgatcgtcg
     8041 gcgccggcgc gaaagttctg ggcggcttca aggttggcga tggcgcgcgc atcggatcga
     8101 atgccatcgt ggtgaaggaa gtgccggcgg gcgcgaccgt cgtcgggatg cccggcagga
     8161 tgctcgagaa ggccggaacc cgggaagccg agcgcttcgc cgcctacgcc gtggtccagg
     8221 agcaggatga cccgtacgcg aaggcgatcc agcaactcgt cgagcactcc caggagctcg
     8281 agcgcgcggt cgcggcgctg aacgagaagc tcgccaggct tgaaaacgcc gaccgcgagc
     8341 acccgctgcg cgtagtccaa aagtaaccta acccgttatt tccttatagg aaactaatcc
     8401 gacagattta gtcggaattg gggtatactt gactgcgtta atcgggtatt agaaatagac
     8461 gcagaaaggc agaggtacct atgcgactca ctacgaaggg caggtttgcc gtaaccgcga
     8521 tggtcgacct ggcgctgcgc cagaaccgcg gcccggtgac gctcgccgcg atcagcgagc
     8581 gccagcacat ttcgctctcc tacctcgagc agttgttcgg caagctgcgc cgcgcctcgc
     8641 tcgtctcgag cgtgcgcggc cccggcggcg gctacaacct tgcgcagccg ccgaacggca
     8701 tttcggtcgc cgacatcgta gccgcggtgg acgagccgct cgacgcgacg cagtgcggcg
     8761 gcaaggagaa ctgccacgac gacaagcgct gcatgaccca cgacctgtgg gccacgctga
     8821 acgagaagat gtacgagtat ctctcgtcgg tgtccctcgc cgatctcgtc gcgcaccaga
     8881 gcggcaagcc ggtgtcggtg atcaaggatt tccggccgtc ttccgagaaa gtcgctgcat
     8941 gaggctgccg gtctatctcg actattcggc gacgactccg gtcgatcccc gcgtcgccgg
     9001 gaagatgatc ccctatctca ccgagttctt cggcaacgcg gcgagccgct cgcacgcgtt
     9061 cggctggaag gcggaagagg cggtggagga ggcgcgcggg cacgtcgccg cgctgatcaa
     9121 cgccgatccc aaggagatcg tctggacctc gggcgcgacc gaaggcaaca acctcgcgat
     9181 caagggcgcg gccaatttct acaagaccaa gggaaagcac atcatcacgc agaagaccga
     9241 gcacaaggcc gtgctcgaca cctgccgcga gctcgagcgc cacggcttcg aggtcacgta
     9301 cctcgaagtg gattccaatg gattggtttc cgtggaaagg gtgaaagcgg cgctgcgccc
     9361 ggacaccatc cttgtctcga tcatgatggt gaacaacgag atcggcgtga tccagccggt
     9421 ctgggagatc ggcgagttgt gccgcgccaa gggcatcatc ttccactgcg acgcggtgca
     9481 gggcggcggc cgtctcgaca tcgacgtgca gaagttcaag gtcgatctcc tcacgatcac
     9541 ggcgcacaag atatacgggc cgaaaggcat cggcgcgctc tacgtccggc gcaagccgcg
     9601 cgtgcgcatc gaggcgcaga tccacggcgg cggccacgag cgcggcttcc gctcgggcac
     9661 gctcgcgacg caccagatcg tcggcttcgg cgaggcggcg cgcctggcga aggccgagat
     9721 ggcgagcgag aacgagcgca tccgcgcgct gcgcgaccgg ctgtacaagg gcatccaggg
     9781 catcgaggaa gcctatgtca acggcgacct caagcagcgc atcccgcaca acctcaacgt
     9841 ctcgttcaat ttcgtcgagg gcgagtcgct gatcatggcg gtgaaggatc tcgcggtgtc
     9901 gtccggctcc gcgtgcacct cggcgtcgct cgagccctcg tacgtgctgc gcgcgctcgg
     9961 ccgctcggac gagctcgcgc acagctcgat ccgcttcacc ctcgggcgct ttaccaccga
    10021 ggaggagatc gacttcgcaa tagaattgat caagcggaag gtggcgaagc taagagagct
    10081 ctcgccgctg tgggagatgc accaggaagg catcgacttg aatactgtgc agtgggctgc
    10141 acattaaggg tggcctagag gccggcctga aatcaggagt cagacatggc atacagcgac
    10201 aaagtcattg accactacga aaacccgcgc aacgtcggct cgttcgacaa ggcggacgac
    10261 gcagtcggca ccggcatggt cggcgccccc gcgtgcggcg acgtgatgaa gctgcagatc
    10321 aaggtcggcg ccgacggccg catcgaggac gcgcgcttca agacctacgg ctgcggctcg
    10381 gccatcgcct cgagctcgct cgtcaccgag tgggtgaagg gcaagacgct cgagcaggcc
    10441 accacgatca agaacacgca gatcgcgcaa gagctgtcgc tcccgccggt gaagatccac
    10501 tgctcgatcc tcgccgagga cgcgatcaag gcggcggtgg cggactaccg caagaagcac
    10561 gaagtctctg aaagcaagga caagaaggta gcctgatggc gattacgctg acacccaacg
    10621 cggccggcca tgtgcagcgc tcgctctcca agcgcggcaa ggggctcggc ctgcgcgtgg
    10681 gcgttcgcac ctcgggctgc tccggcctcg cctacaagct ggagtttgcc gaccaggcga
    10741 accccggcga cctcgaattt gagagccacg gcgtcaaggt gctcgtggac ccgaagagcc
    10801 tgcagtacat cgacggtacc gagctcgact acacccgcga gggcttgaac gagggcttca
    10861 agttcaggaa ccccaaggtc aaggacgaat gcggctgcgg ggaatccttt aacgtagcct
    10921 agattgcacg acgctcgcgc gaccggccga cacacggtat acgtgtgcgg aggcgcgtga
    10981 tgcgtcgatg cggatgcaaa atcacttcga cttgttcggg ctagcgcctg catttgcagt
    11041 cgaggccgaa gcgctcgagc ggtcctaccg cgagatccag tccagggtcc accccgaccg
    11101 ctttgcgcac gccggcgacg ccgagcgccg cgcgtccctg cagtggacca cacgcgtaaa
    11161 tgaagctttt cagatactga aaaaccccgt gacgcgcgcg aagcatttgc tcgagctcca
    11221 cggtgtggat gtcgccttcg agaccaatac cgccatgccc gcggactttc tggtgcagca
    11281 gatggagctg cgcgaaacgc tcgagaacgc cagggatgca gcggcgctag acgcgctgcg
    11341 cgacagtctg ggcaaggaca agcgcgcctt ggaaaagcag atcggcgagg ccatcgatcg
    11401 caggaaggac tacgccggcg cggccgggct ggtgaggaaa ctgcaattcc tagacaggct
    11461 cgataccgaa atcgattcag cgtatgaagg ggttgaatag atggcgctga tgcagatctc
    11521 ggaaccgggc atgtcgcccg agccgcacca ggcgcggctc gcggtcggca tcgatctggg
    11581 caccacgaac tcgctcgtgg cgaccgtgcg cagcggggtg ccggcggtgc tgccggacgc
    11641 tcacggccgg ccgctgctgc cgtcgatcgt gcggtatttt ccggacgggc gggcggaagt
    11701 cggttatgca gcgcaggctc gccaggccga agatccgaaa aacaccgttg tttcagtgaa
    11761 gcggtacatg ggccgcgggg tcaaggacgt cgcccatatc gagaacgcgc cgtacgactt
    11821 cgtcgatgcg ccggggatgc ttcaggtccg caccgtggcg ggcgtgaaga gcccggtcga
    11881 agtttcggct gacatcctgc gagtactgcg ggagcgtgcg gagctgtccc tgcaaggcaa
    11941 tattttcgga gcggtcatca ccgttcccgc ctacttcgac gacgcccagc gccaggccac
    12001 caaggacgca gggcgcattg ccggcctgaa tgtcctcagg ttgctgaacg agccgacggc
    12061 ggccgccatc gcctatgggc tcgagcacgc cgccgagggc acctacgcgg tctacgatct
    12121 cggcggcggc accttcgaca tctcgattct caaattatcg aaagaggtgt tcgaggtcgt
    12181 cgcgaccagc ggcgattcga tgctcggcgg cgacgacttc gaccagcgcg tgttctgctg
    12241 ggcggtagag caggcgaagc tgcccccgct cgacccgcgc gacatgcgcc ggctgctcgt
    12301 caaggcgcgc gaagcgaagg agcacctgac ggtgcacggc gaggccccga tcacggcgaa
    12361 gctctcgacc ggcgaggtcg tggacctgtc cctggatacc gagacgtttt gcagcatcac
    12421 ccagaccctg gttcagaaga ccctcggccc ggtgcgcaag gcgctgcgcg acgcgggcct
    12481 gcgcgtcgag gacgtcaagg gcgtggtgat ggtcggcggc gcgacgcgca tgccgcagat
    12541 ccagcgcgcg gtcggcgagt tcttccggcg cgatccgctc aacaacctcg acccggacaa
    12601 ggtggtggcg ctcggcgcgg cgatgcaggc gaacgtgctc gccggcaaca agccgcaggg
    12661 cgaggactgg ctgctgctcg acgtgatccc actgtcgctc ggcatcgaga ccatgggcgg
    12721 cctcaccgag aagatcatcc cgcgcaacac caccatcccg gccggaaggg cgcaggagtt
    12781 c
//
LOCUS       DATBSZ010000062         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_76009, whole genome shotgun sequence.
ACCESSION   DATBSZ010000062 DATBSZ010000000
VERSION     DATBSZ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76009"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VIG70_04145"
     CDS             <1..951
                     /locus_tag="VIG70_04145"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY8249774.1"
                     /translation="TMTKVAGPEMRKAGYSPALWTGTLAAGGTLGILIPPSVILVIYA
                     VFTEQSIGALFIAAIIPGAIAAIQYMLVVNLYVRFVPGAVVRQEKAPWRERLVATHQV
                     WPVLLVFGLVVVGIYRGWFSATEGAGVGAFATFVLAIWRGGMRWQGIRESLLATGETT
                     AMMYLIMLGAELFSAGLASSQMPTLFAQKVAGLEASPVVIMMAILLAYFILGCFMESL
                     AMVLLTLPVFIPLVLALDFGMTKDAVLVWFGILVLVSVEVGMISPPFGLNLFLINSMA
                     KNVPMGETYKGVLGFCAMDAIRLAALIFFPALALWLPALK"
     gene            complement(944..2227)
                     /locus_tag="VIG70_04150"
     CDS             complement(944..2227)
                     /locus_tag="VIG70_04150"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY8249775.1"
                     /translation="MRKFLARLEERGELRKITESVDPKYEISAYLCLTGAGPALRFGR
                     VAGSEISIVGNLLCSRERIALGLATSTIELQRKIVAAIDHPRNPRTTERAPCQEIVEE
                     KPDLAKLPIPTFFEHETGPYVTAGIIAARDVESGEANLSYARLKPLGANRAFIGIAPN
                     HHLAVMARRASQLPIAVTLGNSPAVSIAAALYLRFGEDELRVAAALASEELEVVKCTS
                     ADILVPAHCEMVLEGVLKSGEMIEEGPVSEYHGLYENYGRGYVVEFNRLTRRRDAMLQ
                     VIQPGYHPEHVWIGGEAIAAGLARLLGARVAVTPGGAGRLHAVIAVKDGAREAMRGAW
                     SAVNLIKMVTVVDEDVDPWDPVQVELAIATRMRAERDVLVVPSMPSNRSDPLARNGMV
                     GKLGIDATRKSGDRKDWRRAEPPAEVLERVRRRLT"
     gene            2263..>2462
                     /locus_tag="VIG70_04155"
     CDS             2263..>2462
                     /locus_tag="VIG70_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249776.1"
                     /translation="MEQAKPRLLIPGLRAFYEWAEPVSWLLVRVTAGLMLIPHGWPKL
                     MMGVTATANMALVKRGIAPAEPL"
BASE COUNT          345 a          842 c          806 g          469 t
ORIGIN      
        1 accatgacca aggtggccgg gccggaaatg cgcaaggcgg gttactcgcc cgcgctgtgg
       61 accggcacgc tcgccgccgg cggcacgctc ggcatcctga ttccgccgtc ggtgattctc
      121 gtcatctatg ccgtgttcac cgagcagtcg atcggagcgc tcttcatcgc cgcgatcatt
      181 cccggggcca ttgccgccat ccagtacatg ctggtggtga acctgtacgt gcgcttcgtg
      241 ccgggcgcgg tggtgcgcca ggagaaagcg ccctggcgcg agcgactggt cgcgactcac
      301 caggtgtggc cggtgttgct ggtgttcggg ctggtcgtgg tgggaatcta ccgcggctgg
      361 ttctcggcta ccgagggtgc cggcgtcggc gcgtttgcca cgttcgtgct cgcgatctgg
      421 cgcggcggca tgcgctggca gggcattcgc gagtcgctgc tcgccacggg cgagaccacg
      481 gcgatgatgt acctgatcat gctcggcgcg gagctgttct ccgccgggct cgcgtcctcg
      541 cagatgccga cgctgttcgc gcagaaggtc gctggcctcg aggcttcgcc ggtggtgatc
      601 atgatggcga tcctgctcgc ctacttcatc ctcggctgct tcatggagtc gctcgccatg
      661 gtgctgctga cgctgccggt attcattccg ctggtgctcg cgctcgactt cggcatgacc
      721 aaggacgcgg tgctggtgtg gttcggcatc ctggtgctgg tgagcgtgga agtgggaatg
      781 atcagcccgc cgttcgggct gaacctgttt ctcatcaact cgatggccaa gaacgtgccg
      841 atgggtgaga cctacaaagg cgtgctcggc ttctgcgcca tggacgcgat ccgcctcgcg
      901 gcgctcatct ttttccccgc cctggcgctc tggctgccgg cgctcaagta agccggcgcc
      961 gcaccctttc cagcacctcg gccggcggct cggcgcgccg ccaatctttc cggtcgccgc
     1021 tcttccgcgt cgcgtcgatg ccgagctttc cgaccatgcc gttgcgcgcg agcggatcgg
     1081 aacggttgct cggcatgctg ggcacgacga ggacgtcgcg ctcggcgcgc atgcgcgtgg
     1141 cgatcgcgag ctcgacctgc accgggtccc agggatcgac gtcctcgtcc accaccgtca
     1201 ccatcttgat caggttgacc gcgctccatg ctccccgcat ggcttctctc gcgccgtcct
     1261 tcaccgcgat caccgcgtgc aggcgcccgg cgcctcccgg cgtgaccgcg acccgcgccc
     1321 ccagcaggcg cgcgagcccc gccgcgatcg cctcgcctcc gatccagacg tgctcggggt
     1381 ggtagccggg ctggatcacc tgcagcatcg cgtcgcgcct gcgcgtcagt cggttgaact
     1441 cgaccacgta gccgcggccg tagttctcgt acaggccgtg gtattccgag accggccctt
     1501 cctcgatcat ctcgccgctc ttcagcacgc cttccagcac catctcgcag tgcgccggca
     1561 cgaggatgtc cgcgctcgtg cacttcacga cttcgagttc ctcgctagcg agcgccgcgg
     1621 cgacccgcag ctcatcctcg ccgaacctca ggtagagagc ggcggcgatc gataccgccg
     1681 ggctatttcc caacgtcacc gcaatcggca gctgggaagc gcggcgggcc atcactgcca
     1741 gatgatgatt cggcgcgatt ccaatgaaag cgcgattggc ccccagcggc ttgagccgtg
     1801 catacgacag gttcgcttcg ccgctttcca cgtcgcgcgc ggcgatgatg cccgcggtga
     1861 catacggacc ggtctcgtgc tcgaagaacg tcggaatcgg cagcttcgcc aggtccggct
     1921 tctcctcgac gatctcctgg cacggcgcgc gctcggtcgt ccggggattt cttgggtggt
     1981 cgatcgcggc gacgatcttg cgctgcagct cgatcgtgga ggtggccaag cccagcgcaa
     2041 ttcgctccct ggagcagagg aggttgccga cgatgctgat ctcgcttccg gcgacgcgcc
     2101 cgaacctcag cgccggcccg gcgccggtca ggcacagata cgccgagatc tcgtacttcg
     2161 gatccacgct ttcggtgatc ttgcgcagct cgccgcgctc ctcgagccgc gccaggaatt
     2221 tgcgcatgcg ccataatcta gccgaaaggg gagggaaatc tcatggaaca ggcgaagccg
     2281 aggctgctga tcccgggatt gcgcgcgttc tacgaatggg ccgagccggt ctcgtggttg
     2341 ctggtgcgcg tcaccgctgg cctcatgctc atcccgcacg gctggccgaa gctgatgatg
     2401 ggcgtgaccg cgacggcgaa catggcgctc gtgaagcgcg gcatcgcgcc cgccgagccg
     2461 ct
//
LOCUS       DATBSZ010000063        10063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1783131, whole genome shotgun sequence.
ACCESSION   DATBSZ010000063 DATBSZ010000000
VERSION     DATBSZ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783131"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..2104)
                     /locus_tag="VIG70_04160"
     CDS             complement(200..2104)
                     /locus_tag="VIG70_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEY8249777.1"
                     /translation="MAEEYIERPQPKGSFSRTIAVLQGTLITVVVGFVLDLHRSVLKL
                     NLYTEQFLLVVLGLAIALCFLMTSKNRMLDAAAALAGLGICGYLAARYPQLSTELTSR
                     PLDGIITSGILAALVLEGTRRMAGFSLVAFTLGGVVYAMVGHHLPGVFQARPVDFTRL
                     LVYLNLDTNALLGTSLQIAIIVVVPFILLGNVLGRCGGSEFFTDLARAWMGRYRGGSA
                     KVAVVGSSFFGMISGSAVANVSAVGVITIPLMKRSGFPAQIAAAIEAVGSTGGQLMPP
                     VMGAAAFLMAEVLQVPYAEVMIAAIIPAFLYYLALFLQVDVEAAKRGIAGEPAERLPR
                     LAAVLKSGWYFPIPFAVLVYALVALNWQAEYAALTATALLIGLSLAFGYKGSRISPKQ
                     VFTAIVSTGGAVVDLILICAVAGMFIGILNISGLAFGMTLQLLAITGESLALMLVVTA
                     LMAIVLGLGLPTVGVYIIMATLIAPALVKVGVAPMAAHMFLLYFGIMSMVTPPVALSA
                     FAAANIAGADVDKTGWTATRIGWAAYIVPFLFALSPSLLMRGDPLGIAWAVATAALGL
                     WLGTIGVVGYFYRPIVGWMRAAFIAAGVLTLIPADMFPGAILTDVVGIALGAALLARE
                     FYDRRRALHI"
     gene            complement(2110..3054)
                     /locus_tag="VIG70_04165"
     CDS             complement(2110..3054)
                     /locus_tag="VIG70_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY8249778.1"
                     /translation="MKRLLVALCAAALPAALNAQALGFGSAPQGSIGYNMSSAIAKVM
                     AEDAKLQSRVQPYSGSSAVLPLVNSGELDLAVCNVLEIEEATRGEGPYNGRRQDKLRV
                     LAVIFPLLSSIFVRNDSPIKSLADLKGKRIPAGFSAQVTLDRIIDAVLANGGVERKDI
                     VPVLVPNVIRGADDFAEGKIDGGFFALGAAKVLEVHKSTGGIRYLPLSDDPAAVARMK
                     KHMSYAYVTTVNPSPAFAGVATPTKLMAYDYLVAVGAHVKDDQAYTIAKAMYEGKPKL
                     IESLGAFRGFNPELMNRAMPATFHPGAVKFYQEKGIAK"
     gene            complement(3069..3458)
                     /locus_tag="VIG70_04170"
     CDS             complement(3069..3458)
                     /locus_tag="VIG70_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8249779.1"
                     /translation="MIDHIDHIVLTTRDKDACIRFYGQVLGMELVRFKTPTEERLALK
                     FGEQKINLHEWGREFTPRAHVAVPGSLDLCFIASISLEKVIENLEKSSIRIIEGPVAK
                     TGARGPIRSVYVRDPDLNLVEISVYNK"
     gene            complement(3455..4630)
                     /locus_tag="VIG70_04175"
     CDS             complement(3455..4630)
                     /locus_tag="VIG70_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY8249780.1"
                     /translation="MSGPLAGVRVLELGSLVAGPFCGKTLADFGAEVIKIEPPGEGDP
                     LRKWRRMRNGTSLWWQVQSRNKRSVTCDLRRPEGQEIVRRLARASHIVVENFRAGALE
                     KWNLGWGVLSRENPKLVLVRISGYGQDGPYSERPGFAAIAEGMAGFRYITGFPDRPPA
                     RPNLSIGDTIASLHGVIGALMALHSGKGQVVDVALYEAMFNCMESLVADYDGDGYVRE
                     RTGSTLPGIAPSNLYPCKDGSYVLIAGNADSLYHRLMTAIGREDLRDDPALARNDGRA
                     AQMERIDAAIASWTQVRELTEILKTMEKAEVPAGRIYSAADIAADPHFAARGMIEEVI
                     AGDGEPLKVPGIVPKLSATPGAIRSPAPKLGEHTDGVLEELGYSRKAIQELRRSKII"
     gene            complement(4627..5058)
                     /locus_tag="VIG70_04180"
     CDS             complement(4627..5058)
                     /locus_tag="VIG70_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl dehydratase"
                     /protein_id="HEY8249781.1"
                     /translation="MKTVGRGFSFEELKVGMRFRTHRRTITEADLAAFVNLSWLTEEL
                     FAVEEEGKQRLVPGALVYAFAEGLLLPTMQDTGLAFLNATLDVKAPTLVGDTIHVECE
                     VVEHRLTSKGDRGLVRFANRVTNQRNEIVLEYNPLRLLKKK"
     gene            5169..5612
                     /locus_tag="VIG70_04185"
     CDS             5169..5612
                     /locus_tag="VIG70_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249782.1"
                     /translation="MLFSVLLGVLGVVGAGAGVGLLVLPLDMPPGVLLPVLPPMLPLV
                     LPPLLAPAPAPLRASRRQVSRCAPIRSLHLSLASTLVPSEPDALPPAAAPPLTPEPDV
                     VPLAPVLPEVDPDVWAMVTEDRASSAAAVAAVSTFNIMEDLLRVG"
     gene            5717..6184
                     /locus_tag="VIG70_04190"
     CDS             5717..6184
                     /locus_tag="VIG70_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8249783.1"
                     /translation="MERIEKSIEVTCPVRTVYNQWTQFEEFPRFMAGVKEVRQLDDTH
                     VHWHAEVWGKDKEWDSEITEQVPDQVIAWRSTSGTPNAGMVRFEPVSHDRTVVRLIME
                     YEPQGAVEKAGDAIGVFSARVQNTVEDFKKFIEKRGTETGAWRGEVHGGRKPA"
     gene            complement(6205..6633)
                     /locus_tag="VIG70_04195"
     CDS             complement(6205..6633)
                     /locus_tag="VIG70_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEY8249784.1"
                     /translation="MTAVMAFLHHVAAFALVAALAVEFVLIRGELTAATARRLASADM
                     ILGVSAGVLLAAGLLRVFYFEKGAAYYFHSATFIAKLTLFVLVAVLSIYPTVKFISWR
                     KGIDASSVPAIRRIIHLELAGIVLILLFAALMADGIGYWG"
     gene            6848..7384
                     /locus_tag="VIG70_04200"
     CDS             6848..7384
                     /locus_tag="VIG70_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEY8249785.1"
                     /translation="MVSLETLPTRISQRIVSIRGQRALLDADLAALYGVETRVLLQAV
                     RRNHSRFPRDFMITLRNQDVAALRSQFVISKRAGRGGRAYAIHAFTDYGAIMAASVLN
                     SRRAIEVSIFVVRAFVQVRESLAERKELGRRLDELEGRVEQRLGAHDRAISEILEAIR
                     QLMAPPAQRPKPGIGFVR"
     gene            complement(7381..8520)
                     /locus_tag="VIG70_04205"
     CDS             complement(7381..8520)
                     /locus_tag="VIG70_04205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8249786.1"
                     /translation="MDFSLSPEQERIREAIGKLCQRFPDEYWLERDREGGFPADFHQA
                     FARDGWLGIAMPEDYGGSGLGISEAAVMMQAIAESGAGFSGASAVHMNIFGLNPVVVY
                     GSEEQKKRMLPPLVAGRERACFAVTEPNTGLNTKKLKTKAEKRGDKYYVSGQKVWIST
                     AQVAEKVLLLAAADSGLTLFYTDLDRKYVEVREIDKMGRHAVDSNQVFFDGLPVPERD
                     RIGEEGKGFEYILHGMNPERILIAAELVGLGRCAVGRAAKYAKERVVFDRPIGQNQAI
                     QHPLAANWMALEAANLMAFKAASLYDDGKPCGAEANAAKYLAGEACFDACQQAVMTFG
                     GFGYAREYHVERYLRESFVGRIAPVSRELILCFIAERVLGLPKSY"
     gene            complement(8523..9281)
                     /locus_tag="VIG70_04210"
     CDS             complement(8523..9281)
                     /locus_tag="VIG70_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8249787.1"
                     /translation="MPQKPPAAAVSDLAPSGTLRAAINVGNAVLAARPKEAGGAPTGV
                     SVDLAHELARRLGVPLHLIMFDAAGRVVEAGKAGDWDIAFVALDPARATEIAQSPPYV
                     QIEGAYLVRSDSPLKSNEEVDRKGHRVVVARNSAYDLYLTRNLKQAEIVRTPTSATVV
                     DTFMKESLEVAAGVKQQLENDARRVPGVRLLPGRFMVINQAMASQRVTETRGAAGARY
                     VREFIEEMKASGFVAKALERHNQTAVVAPAGDAK"
     gene            complement(9337..9702)
                     /locus_tag="VIG70_04215"
     CDS             complement(9337..9702)
                     /locus_tag="VIG70_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8249788.1"
                     /translation="MDTAIKYLIRQSDLEPYSPANHTGTRNYRLVASKNLEVLIGEIE
                     RGEGALPHSHPGIEQVCYLLEGTAHVEVADEKFEMRAGEACFFPADKPHLFRATSQKV
                     RVMVIYSPPYLENPANVRR"
     gene            complement(9678..>10063)
                     /locus_tag="VIG70_04220"
     CDS             complement(9678..>10063)
                     /locus_tag="VIG70_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8249789.1"
                     /translation="GLHMKEKKIRGRMLISTSLHAESPRNLPHYSAAKAGQTMLVKEL
                     ARALGPHGIRVNAIVPGAIPGEGFKGLPGMEKTIPLGRFGTPADVAAMALAVLSERFG
                     AYVTGASILVDGGIALHNWIPPSNI"
BASE COUNT         1673 a         3352 c         3344 g         1694 t
ORIGIN      
        1 gcgacacggt ccgcgatcgc tttttcagaa atctgctctt tcaccgaagc gggagcgagg
       61 cgcaccagct cctgatagct cacgcggttc ccctcgcggt cgaggtggtg cgcatacatc
      121 tcgtcgcggc gcgcgtggcc cgccatttcc tcggccgggc tgttgagggc gggactgtgc
      181 gaggtgccga acgcggcgac tagatgtgca atgctctcct ccggtcgtag aactcgcgcg
      241 ccaggagcgc tgcgccgagc gcgatgccga cgacgtcggt gaggatcgcg ccggggaaca
      301 tgtcggccgg aatgagcgtc agcacgccgg cggcgatgaa cgccgcgcgc atccatccga
      361 cgatcgggcg gtagaagtag cccaccacgc cgatcgtgcc gagccacagc ccgagcgcgg
      421 cggtggccac cgcccaggcg atgccgagcg gatcgccgcg catcaggagg ctgggcgaga
      481 gggcgaagag gaacggcacg atatacgccg cccagccgat gcgcgtcgcg gtccagccgg
      541 tcttgtccac gtcggcgccg gcgatgttgg ccgcggcgaa ggccgacagc gccaccggcg
      601 gcgtgaccat cgacatgatg ccgaagtaga gcaggaacat gtgcgccgcc attggcgcga
      661 cgccgacctt gaccagcgcc ggggcgatca gcgtcgccat gatgatgtac acgccgaccg
      721 tgggcagccc caggccgagc acgatcgcca tcagcgcggt gaccaccagc atgagcgcca
      781 ggctctcgcc ggtgatcgcg aggagctgca gcgtcatgcc gaacgcaagg ccgctgatgt
      841 tcaggatgcc gatgaacatg ccggccactg cgcagatcag gatcaggtcg accaccgcgc
      901 cgccggtgga gacgatagcc gtgaatacct gcttcgggga gatccgcgag cccttgtagc
      961 cgaacgccag cgacaggccg atcagcaacg cggtcgccgt cagcgcggcg tactccgcct
     1021 gccagttgag cgcgaccagc gcgtacacca gcaccgcgaa cgggatcggg aagtaccagc
     1081 ccgatttcag caccgcggcg agccgcggca ggcgctcggc cggctcgccc gcgatgccgc
     1141 gcttcgccgc ctccacgtcc acctgcagga agagcgcgag gtaatagagg aacgcgggga
     1201 tgatcgccgc gatcatcacc tcggcgtagg gcacctgcag cacctccgcc atgaggaagg
     1261 ccgccgcgcc catcaccggc ggcatgagct ggccgccggt cgagccaacc gcctcgatcg
     1321 ccgcggcgat ctgcgccggg aagcccgagc gcttcatcag cggaatggtg atcacgccca
     1381 cggcgctcac gttcgccacc gcgctgcccg agatcatgcc gaagaagctc gagccgacca
     1441 ccgcgacctt ggccgagccg ccgcgatagc gccccatcca ggcgcgcgcg aggtcggtga
     1501 agaactccga gccgccgcag cgcccgagca cgttgccgag caggatgaac ggcaccacga
     1561 cgatgatggc gatttgcagc gaggtgccga gcagcgcgtt ggtgtccagg ttgaggtaga
     1621 cgagcaggcg cgtgaaatcc accggccgcg cctggaacac gccgggcagg tggtgaccca
     1681 ccatggcgta caccacgccg ccgagcgtga aagcgaccag cgagaatccc gccatgcgcc
     1741 gcgtgccctc gagcacgagc gccgcgagga tgcccgaggt gatgatgccg tcgagcgggc
     1801 gcgaggtgag ctcggtcgag agctgcgggt agcgggccgc aaggtagccg cagatgccca
     1861 gcccggcgag cgccgccgcg gcatccagca tcctgttttt cgaagtcatc aaaaaacaca
     1921 gggcgatcgc aagccccaag accacgagca ggaactgctc ggtatagagg ttgagcttga
     1981 ggacgctgcg gtgcaggtcc aggacgaagc ccaccacgac ggtgatgagc gtgccctgga
     2041 ggacggcgat ggttctcgag aaagaacctt tcggctgagg acgctcgatg tactcctcag
     2101 ccattcaact tacttcgcta tgcctttctc ttggtagaac ttgaccgccc ccgggtggaa
     2161 ggtcgccggc atcgcgcggt tcatgagctc ggggttgaag ccgcggaacg cgcccaggct
     2221 ctcgatcagc ttgggcttgc cctcgtacat cgccttggcg atcgtgtagg cctggtcgtc
     2281 cttgacgtgc gcgccgaccg ccaccaggta gtcgtaggcc atcagcttgg tcggcgtcgc
     2341 cacgccggcg aacgccgggc tcgggttcac ggtggtcacg taggcgtagc tcatgtgctt
     2401 cttcatgcgc gccaccgccg cgggatcgtc cgagagcggc aggtagcgga tgccgccggt
     2461 cgacttgtgc acctccagca ccttggccgc gccgagcgcg aaaaagccgc cgtcgatctt
     2521 gccctcggcg aagtcgtcgg cgccgcggat cacgttcggc accagcaccg gcacgatgtc
     2581 cttgcgctcg acgccgccgt tcgcgagcac cgcgtcgatg atgcggtcga gggtcacctg
     2641 cgccgagaag cccgcgggga tgcgcttgcc cttgaggtcc gccagcgact tgatgggcga
     2701 gtcgttgcgc acgaagatgc tcgaaagcag cgggaagatc accgccagca cgcgcagctt
     2761 gtcctgccgg cggccgttgt aggggccttc gccccgggtc gcctcctcga tctcgagcac
     2821 gttgcacacc gcgaggtcga gctcgcccga gttgacgagc ggcagcaccg cgctcgagcc
     2881 cgagtagggc tggacccggc tctgcagttt tgcgtcctcg gccatcacct tggcgatcgc
     2941 cgagctcatg ttgtagccga tcgagccctg cggcgcgctg ccgaagccca gggcctgggc
     3001 attgagcgcg gcaggcagcg ctgccgcgca cagcgcgacg agcagtcttt tcatggttgc
     3061 ctcctcctct acttattgta gaccgagatc tcgaccaggt tcaggtccgg gtcgcgcacg
     3121 tacaccgagc ggatcggccc gcgcgcgccg gtcttcgcca ccggcccttc gatgatgcga
     3181 atgctgcttt tctccaggtt ctcgatcact ttctcaagcg aaatcgaggc aataaagcaa
     3241 aggtcgagcg aacccggaac cgcgacgtgc gcgcgcggcg tgaactccct gccccactcg
     3301 tgcaggttga tcttctgctc gccgaatttc agggcgagac gctcctcggt cggggtcttg
     3361 aacctcacca gctccatgcc gagcacctgg ccatagaagc ggatacacgc atccttgtcc
     3421 cgggtagtca ggacgatgtg gtcgatgtgg tcgatcatat gattttgctt cgcctcagct
     3481 cctggatcgc cttgcggctg tagcccaatt cttccagaac cccatccgta tgctcgccga
     3541 gcttcggcgc cggcgagcgg atggcgccgg gcgtggccga gagcttcggc acgatccccg
     3601 ggaccttgag cggctcgccg tcgccggcga tgacctcctc gatcatgccg cgcgcggcga
     3661 aatgcggatc ggcggcgatg tccgccgcgc tgtagatgcg cccggccggg acttccgctt
     3721 tctccatggt ttttagaatt tctgtcagtt ctctgacctg ggtccaagaa gcaatggctg
     3781 cgtcgatgcg ctccatctgc gcggcacggc cgtcgttgcg cgcgagcgcc gggtcgtcgc
     3841 gcaggtcttc gcggccgatc gcggtcatga ggcggtggta caggctgtcg gcgttaccgg
     3901 cgatcagcac gtagctgccg tccttgcagg gatagaggtt ggagggcgcg atgccgggca
     3961 gcgtgctgcc ggtgcgctcg cgcacgtagc cgtcgccgtc gtagtcggcg accaggctct
     4021 ccatgcagtt gaacatcgcc tcatagagtg cgacgtcgac cacctgcccc ttgcccgagt
     4081 gcagcgccat gagcgcgccg atcacgccgt ggagcgaggc gatggtgtcg ccgatcgaca
     4141 ggttgggccg cgccggtggc cggtcgggaa agccggtgat atagcgaaag cccgccatgc
     4201 cttcggcgat cgcggcgaag cccgggcgct cgctgtacgg gccgtcctgc ccgtagcccg
     4261 agatgcgcac caggacgagc ttcgggttct cgcgcgacag gacaccccag ccaaggttcc
     4321 acttttcgag cgcgcccgcg cggaagttct caaccacgat gtgcgatgcg cgcgcgaggc
     4381 ggcgcacgat ctcctggccc tcgggccggc gcaggtcgca ggtgaccgag cgcttgttgc
     4441 gcgactgcac ctgccaccac agcgaggtgc cgttgcgcat gcggcgccac ttgcgcagcg
     4501 gatcgccctc gcccggcggc tcgatcttga tcacctcggc gccgaagtcg gcgagcgtct
     4561 tgccgcagaa cgggcccgcg accaggctgc cgagctcgag cacccgcacg cccgccagcg
     4621 ggccgctcat ttcttcttca ggagccgcag cgggttgtac tcgaggacaa tctcgttcct
     4681 ctggttcgtg acccggttcg cgaagcgcac caggccgcgg tcgcctttcg aggtgagacg
     4741 gtgctcgacg acctcgcact cgacgtggat ggtgtcgccg acgagcgtgg gcgccttcac
     4801 gtcgagcgtg gcattgagga acgcgaggcc ggtgtcctgc atcgtcggca ggagcagccc
     4861 ctcggcgaaa gcgtacacga gcgcgcccgg caccaggcgc tgcttgcctt cttcctcgac
     4921 cgcgaacagc tcctcggtca gccacgaaag attgacgaac gcggcgaggt cggcctcggt
     4981 gatggtgcgg cggtgggtgc ggaagcgcat ccccaccttc agctcctcga aggagaaccc
     5041 gcggccgacg gtcttcatgc ggcattctag cgccgctact ttttacctcc gcgtgcgccg
     5101 cgaggcgcgg ttgccgggga ttacgccatc cccggcgaac cgcaacttct tgctgagtac
     5161 gctgctactt gcttttctcg gtgctgctcg gggtgctcgg cgtggtcgga gccggcgccg
     5221 gggtcgggct gctggtgctg ccgctcgaca tgccgcccgg cgtgctgctg ccggtgctgc
     5281 cgcccatgct gccgctcgtg ctgccgccgc tgctcgcgcc ggcgcccgcg ccgctgcgcg
     5341 cgtcgcgcag gcaggtctcg cgctgcgcgc cgatcaggtc cttgcacttg tcgttggcat
     5401 cgacgctcgt gccgagcgaa cccgacgcgc tgccgccggc ggccgcgccg ccgctgacgc
     5461 ccgagcccga cgtcgtgccg ctcgcgcccg tgctgccgga agtggatccc gacgtctggg
     5521 cgatggtcac ggaggacaga gcgagcagcg cagctgcggt tgcggcggta agtactttca
     5581 acatcatgga ggatctcctc agagtcggtt gagttgcgat tggactgcgg atgcgttgca
     5641 cgcaagggtc gtgcccgcac cgcgagcgga acgcgtcttg cctctccgaa gaaactcatc
     5701 cacgacagga gtaaccatgg aacggatcga gaagtcgatc gaggtcacct gccccgtgcg
     5761 cacggtctac aaccagtgga cgcagttcga ggagttcccg cgcttcatgg ccggcgtcaa
     5821 ggaagtgagg cagctcgacg acacccacgt gcactggcac gcggaggtct ggggcaaaga
     5881 caaggaatgg gattccgaga tcaccgagca ggtgcccgac caggtgatcg cgtggcgcag
     5941 cacctccggc acgcccaacg ccggcatggt ccgattcgag ccggtgtcgc acgaccgcac
     6001 cgtggttcgc ctgatcatgg aatacgagcc gcaaggcgcg gtcgaaaagg ccggcgacgc
     6061 gatcggcgtg ttcagcgcgc gcgtgcagaa caccgtcgag gacttcaaga agttcatcga
     6121 gaagcgcggc accgagaccg gcgcgtggcg cggcgaggtg cacggcggga ggaagcccgc
     6181 ctagccgcgc attgatcccc gcatttatcc ccaatacccg atgccgtccg ccatcagcgc
     6241 ggcgaacagc aggatcagca cgatcccggc gagctccaag tggatgatgc ggcggatggc
     6301 cggcacgctc gaggcgtcga tgcccttgcg ccaggagatg aacttcaccg tcggataaat
     6361 cgacagcacc gcgaccagca cgaacagcgt gagcttggcg atgaaggtcg cgctgtggaa
     6421 atagtaggcg gcgcccttct cgaaatagaa cacgcgcagc aaccccgccg ccagcagcac
     6481 gcccgccgag acgccgagaa tcatgtcagc gctcgccagc ctgcgcgccg tcgccgcagt
     6541 gagctcgcca cggatcagga cgaattccac cgccagcgcc gcgaccagcg cgaacgcggc
     6601 gacgtgatgc agaaacgcca tgaccgcggt catttgcgtc tttcctttct gatcacgagc
     6661 ttcaagcccg accaggtgtc gtccaccgcg cacaccttga tgtcgaccag gcccatcggc
     6721 aacgccacct cccggatcgt gtcctctttg acgaacagct ctgtgcccgg cctgatccca
     6781 gcttcctcgc caggggcgta cctgaatatc cggtcatcgt tttgcgttgc ggcgcgtttg
     6841 agatcccatg gtatcgctcg agactctgcc gacccggatt tcacagcgga tcgtgagtat
     6901 tcgaggccag cgcgcattgc tggatgcgga cttggccgcg ctgtacggtg tcgagacacg
     6961 tgtgcttctg caggcggtgc gtcgaaatca ctcacgtttc ccgcgcgact tcatgattac
     7021 cttgaggaat caggatgttg cagctttgag atcacaattc gtgatctcaa agcgagccgg
     7081 ccgcggcggt cgcgcctacg ctatacacgc gttcaccgac tacggcgcga tcatggcggc
     7141 atccgttctg aattcgcggc gcgctattga agtatccata ttcgtcgtac gtgcgttcgt
     7201 tcaagttcgc gagtcccttg ctgaacgcaa ggaactcggc cggcgtctgg atgagctcga
     7261 gggcagagtc gaacagcggc tgggtgccca cgaccgtgcg atctcggaga ttctcgaagc
     7321 cattcgacaa ttgatggcac cgccagcaca gcggccaaag cctgggatcg gattcgtccg
     7381 ctagtaggac ttgggcaggc cgagcacgcg ctcggcgatg aagcagagga tcagctcgcg
     7441 gctcaccggc gcgatgcgcc cgacgaacga ctcgcgcagg tagcgctcga cgtggtactc
     7501 gcgcgcatag ccgaagccgc cgaaggtcat caccgcctgc tggcaggcgt cgaagcaggc
     7561 ctcgcccgcg aggtacttcg ccgcgttcgc ctcggcgccg catggcttgc cgtcgtcgta
     7621 aagcgacgcg gccttgaacg ccatcaggtt ggccgcctcg agcgccatcc agttggcggc
     7681 gagcgggtgc tggatcgcct ggttctggcc gatcggccgg tcgaatacca cgcgttcctt
     7741 tgcatacttc gccgcgcggc cgaccgcgca gcggccgagg ccgacgagct cggcggcgat
     7801 caggatgcgc tccgggttca tgccgtgcag gatgtactcg aagcccttgc cttcctcgcc
     7861 gatgcgatcg cgctccggca ccggcaggcc gtcgaagaac acctggttgg agtcgaccgc
     7921 gtgccggccc atcttgtcga tctcgcgcac ttcaacgtat tttcgatcga gatccgtgta
     7981 gaagagcgtc aatccggaat ctgcggctgc aagcagcaac accttctcgg cgacctgtgc
     8041 cgtcgagatc cacaccttct ggcccgaaac gtaatacttg tcgccgcgct tctccgcctt
     8101 ggtcttgagc tttttcgtat tcaagccggt gttcggctcg gtgacggcga agcaggcacg
     8161 ctccctgccg gcgaccagcg gcggcagcat gcgcttcttc tgctcttcgc tgccgtacac
     8221 cacgacgggg ttgaggccga agatgttcat gtgcaccgcg gaggcgccgg agaaaccggc
     8281 gcccgactcg gcgatcgcct gcatcatcac ggccgcctcg ctgatgccga ggcccgagcc
     8341 gccgtagtcc tcgggcatcg cgatgccgag ccagccgtcg cgagcgaacg cctggtggaa
     8401 atcggcggga aagccgccct cgcggtcgcg ctcgagccag tactcgtcgg ggaagcgctg
     8461 gcacagcttg ccgatggctt cccggatgcg ttcctgctcc ggggaaagcg aaaaatccac
     8521 tcctacttgg cgtcgccggc cggcgccacc accgcggtct ggttgtggcg ctcgagcgcc
     8581 ttggcgacga agcccgaggc cttcatttcc tcgatgaact cgcgcacgta gcgcgcgccg
     8641 gcggcgccgc gcgtctcggt gactcgctgg ctcgccatcg cctggttgat caccatgaag
     8701 cggccgggca gcaggcgcac gcccgggacg cggcgcgcgt cgttctcgag ctgctgcttc
     8761 acgcccgccg cgacctccaa gctctccttc atgaacgtgt cgaccacggt tgccgaggtc
     8821 ggagtgcgca cgatctcggc ctgcttgagg ttgcgcgtga ggtagaggtc gtaggcgctg
     8881 ttgcgggcga ccacgacgcg gtgacctttg cgatccactt cttcgttgct cttgagcggc
     8941 gaatcagagc ggacgaggta cgccccttcg atctgcacgt agggcggcga ctgcgcgatc
     9001 tccgtcgcgc gcgccgggtc gagcgccacg aacgcgatgt cccagtcccc ggccttgccc
     9061 gcctcgacga ccctgccggc ggcgtcgaac atgatgagat gcagcgggac gcccaagcgc
     9121 cgcgcgagct cgtgcgcgag gtccaccgac acgccggtcg gcgcgccgcc ggcttccttc
     9181 gggcgcgctg caaggaccgc gttgccgacg ttgatcgccg cgcgcagcgt gccgctcggc
     9241 gcgagatcgg agaccgcggc tgcgggcggt ttttgcggca tgggcgagca agcggccagc
     9301 aggagtgcgg aaaagcagac cagtgcgaaa cgcatgttat ctccgtacgt tggccgggtt
     9361 ctcgaggtag ggcggcgaat agatcaccat cacgcgcact ttctgcgagg tcgcccggaa
     9421 aagatgcggc ttgtcggccg ggaagaagca cgcctcgccc gcgcgcatct cgaatttctc
     9481 gtcggcgacc tcgacgtgcg cggtgccttc gagcaggtag cacacctgct cgatgcccgg
     9541 gtgcgagtgc ggcagggcgc cttcgccgcg ttctatctcg ccaatcaaca cctcgagatt
     9601 ttttgaagca accaggcgat agttgcgcgt gcccgtgtga ttggcgggcg aataaggctc
     9661 gagatcggac tggcgaatca gatatttgat ggcggtatcc agttgtggag cgcgatgccg
     9721 ccgtcgacca ggatcgacgc cccggtcacg taggcgccga agcgctcgga gagcaccgcg
     9781 agcgccatcg ccgcgacgtc cgccggagtg ccgaagcggc cgagcgggat ggtcttttcc
     9841 atgccgggca gccccttgaa tccttctccg gggatggcgc ccgggacgat cgcgttgacg
     9901 cggattccgt gcggccccaa cgcccgtgcg agctctttca ccagcatcgt ctgcccggcc
     9961 tttgcggcgc tgtagtgcgg caggttgcgc ggcgactcgg cgtgcaggga agtgctgatc
    10021 agcatgcgcc ccctgatctt cttctccttc atgtgcaggc cga
//
LOCUS       DATBSZ010000064         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1238513, whole genome shotgun sequence.
ACCESSION   DATBSZ010000064 DATBSZ010000000
VERSION     DATBSZ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238513"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /gene="mutL"
                     /locus_tag="VIG70_04225"
     CDS             <1..256
                     /gene="mutL"
                     /locus_tag="VIG70_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587194.1"
                     /note="This protein is involved in the repair of
                     mismatches in DNA. It is required for dam-dependent
                     methyl-directed DNA mismatch repair. Promotes the
                     formation of a stable complex between two or more
                     DNA-binding proteins in an ATP-dependent manner without
                     itself being part of a final effector complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HEY8249790.1"
                     /translation="ARGVLGEVREYGASQVLSSRQNELLATMACHAAVRAHRALSIAE
                     MNALLREMEETERSGSCNHGRPTWYQLTMSDLDKLFMRGK"
     gene            253..981
                     /locus_tag="VIG70_04230"
     CDS             253..981
                     /locus_tag="VIG70_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8249791.1"
                     /translation="MKVMIVTGGGRGIGAATARLAAERGYAVCINYLKNGKAAEALAR
                     EIGGIAVQGDVASEADVVRLFKTTDEKLGRVSALVNNAGIVDRGSRVEAMSAERIQRM
                     LAVNVTGSFLCAREAVKRMSTRHGGKGGAIVNLSSVAARLGGSGEYVDYAASKGAIDT
                     FTVGLAKEVGAEGIRVNAVRPGVIRTEIHLASGDPARVERIGGTAPLQRAGEAEEIAR
                     TILWLASDEASYITGALLDVSGGR"
     gene            983..1921
                     /gene="miaA"
                     /locus_tag="VIG70_04235"
     CDS             983..1921
                     /gene="miaA"
                     /locus_tag="VIG70_04235"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004527674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY8249792.1"
                     /translation="MAGTAFALLGPTASGKSALAIKVAAKLPVEIVSMDSALVYRGMD
                     IGTAKPGAALRVQVPHHLIDVIDPDQSFSAGRWREDAISTVLNILKRNQVPLLVGGTM
                     LYYRALTAGLDELPQADARIRAAIEAEATRRGWPALHAELEKVDPKAAGRIAPNDAQR
                     IQRALEVWRIAGKPLSALQGIAGNDLPFDLKGIALVPDRAVLHERIGQRFGAMLRLGL
                     VDEVKQLKKKYRLNAAMPSMRAVGYRQVWEYLDGRYDKESLREKAIAATRQLAKRQLT
                     WLRSFPDLLRLDAGGAQDPAIALHLLLDEGRQPVGR"
BASE COUNT          344 a          666 c          704 g          272 t
ORIGIN      
        1 ggcgcgcggg gtgctcggcg aggtccgcga gtacggcgcg agccaggtgc tgtcgtcgcg
       61 ccagaacgag ctcctcgcga ccatggcgtg ccatgccgcg gtacgcgccc atcgcgcgct
      121 cagcatcgcg gagatgaacg ccctgctgcg cgagatggag gaaaccgagc gctcgggaag
      181 ctgcaaccac ggccggccga cgtggtacca gctcacgatg agcgacctcg ataagctctt
      241 catgcgcgga aaatgaaagt catgatcgtc acgggcggcg gccgcgggat cggcgctgcg
      301 accgcgcgcc tcgccgccga gcgcggctac gcggtatgca tcaactatct gaagaacggc
      361 aaggcagccg aggcgctcgc gcgcgagatc ggaggcattg cggtacaagg cgatgtcgcc
      421 tcggaagccg acgtggtgcg gctgttcaag accacggacg aaaagctcgg gcgagtgagc
      481 gctctggtga acaacgccgg catcgtggac cgcggctcgc gcgtggaagc catgtcggcc
      541 gagcgcatcc agcgcatgct ggcggtcaac gtcaccggca gcttcctgtg cgcgcgcgag
      601 gcggtgaagc gcatgtcgac gcgccacggc ggcaagggcg gcgccatcgt caacctgtcc
      661 tcggtggcgg cgcggctcgg cggatcgggc gagtacgtcg actacgcggc ctccaagggc
      721 gcgatcgata cgttcacggt cggactcgcg aaggaagttg gcgccgaggg catccgcgtg
      781 aacgcggtgc ggcccggcgt gatccgcacc gagatccacc tcgcgagcgg cgacccggcg
      841 cgcgtcgagc gcatcggcgg cacggcgccg ctgcagcgcg cgggcgaggc cgaagagatc
      901 gcgcgcacca tcctctggct cgcctcggac gaggcctcct acattaccgg cgcgctgctc
      961 gacgtctccg gcgggcgcta gattggccgg caccgccttc gcgctcctcg ggccgaccgc
     1021 gagcggcaaa tcggcgctcg cgatcaaagt ggcggccaaa ctgcccgttg agatcgtgtc
     1081 gatggattcc gcgctcgtct accgcggcat ggacatcggc accgccaagc ccggcgccgc
     1141 tctgcgcgtg caggtgccgc accatctcat cgacgtcatc gacccggacc agagcttttc
     1201 cgcgggtagg tggcgtgaag atgcaataag tacggtattg aatatcttga agagaaacca
     1261 agttcccctg ctggtcggcg gcacgatgct ctactaccgc gcgctcaccg ccggcctcga
     1321 cgagctgccg caggccgacg cgaggatccg cgcggcgatc gaggccgaag cgacgcgccg
     1381 cggctggccc gcgctgcacg ccgagctcga gaaagtcgac ccgaaggcgg cgggccgcat
     1441 cgcgccgaac gacgcgcagc gcatccagcg cgcgctggaa gtctggcgga tcgccggcaa
     1501 gccgctgtcg gcgctgcaag gcatcgccgg gaacgacctg ccgttcgatc tgaagggcat
     1561 cgcgctcgtc cccgaccgcg cggtactgca tgagcgcatc gggcagcgct tcggcgccat
     1621 gctgcgcctg gggctggtcg acgaagtgaa gcagctgaag aagaagtacc ggctgaacgc
     1681 cgcgatgccg agcatgcgcg cggtcggata ccgccaggtg tgggagtacc tcgacggccg
     1741 ctatgacaaa gaatctctca gggaaaaagc gatcgcggcg acccgacagc tggcgaaaag
     1801 gcagctgacc tggctgcgca gcttccccga cctactccgg ctcgacgccg gcggcgcgca
     1861 ggatccggcg atagctctcc atctgctcct tgacgaaggc cgccagccgg tcgggcgatg
     1921 aggggctgag cgagaacgcc tgccgctcca ttgccgcctg cacgtcggcg cggcccatgg
     1981 cggcgg
//
LOCUS       DATBSZ010000065        14169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1921230, whole genome shotgun sequence.
ACCESSION   DATBSZ010000065 DATBSZ010000000
VERSION     DATBSZ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14169
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921230"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VIG70_04240"
     CDS             <1..567
                     /locus_tag="VIG70_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY8249793.1"
                     /translation="WFWCSKNVLAQNPLIRRIYGRGRLGSKTYTRIMRWNSRVGVTRK
                     LERLIGLHSESVIQDVDIPISRAAEFLAFYSREIQLWPQWVCPIGPQRDSGRFVLYPV
                     KNTWYVNFGFWDVKRTREAHSPGHFNRQIEEKVSELGGIKSLYSDSFFAEADFHRRYG
                     GAAYDALKKKYDPAGAFPRLYDKCVLKA"
     gene            complement(644..1540)
                     /locus_tag="VIG70_04245"
     CDS             complement(644..1540)
                     /locus_tag="VIG70_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY8249794.1"
                     /translation="MKYKDYYKILGVARGAGDDEIKKAYRKLARKYHPDVSKEANAKE
                     KFQEVSEAYETLRDKEKRAAYDSLGSGFRQGQDFRPPPDWFDRFGSGSRAEDLHGVDL
                     GELFESMGIFGRAQRRSKRAFAGEDYEVPVKLTLEEAFRGTEREVHLDNRSFTARIPR
                     GATDGQRLRLRGKGGPGLNGGPAGDLYLQIALEPHALYRASGHDLEIEVPLGPWEAAL
                     GAQVEIPTLEGRVTMKVPPGSNAGQKLRLAGKGLPKPGGGAGDLYAVLTIAVPATLTE
                     REEKLYQELREASRFDPRARFK"
     gene            complement(1567..2442)
                     /locus_tag="VIG70_04250"
     CDS             complement(1567..2442)
                     /locus_tag="VIG70_04250"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8249795.1"
                     /translation="MALRPFLLLALLSVLPGPVPAQDRFFDSGGVRIRYVDRGAGPPV
                     VLVHGFTGTIERSWIGTGVLPELAREHRVLAFDLRGHGHSDKPHDPRAYDELALDVIR
                     LMDHAGVARAHLVGYSLGGIIAARLLVTHPERFASALLGGAAYRRARSESADEATEAA
                     AREIESGVYRALIVSTAPTDEPPPGEDAIRARSREIAASADVRAHAALMRARRALLVS
                     DAEIAAVRVPVLAVVGSADPALPRVQAMAKRWPGLRLEVVPGAAHPTVHERGLPRHPA
                     FLEAIRRQVGASLQR"
     gene            2484..2822
                     /locus_tag="VIG70_04255"
     CDS             2484..2822
                     /locus_tag="VIG70_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249796.1"
                     /translation="MARKRKGAARKAPAANKAQATRRRLEAEARALKKRLDALMASAR
                     KAEVAARASTMQQIRVLQKRQAAARVVLAKLGRQGAAASGPILVGLQKAWRDIDVAVR
                     EGAKRFRETS"
     gene            2827..3150
                     /locus_tag="VIG70_04260"
     CDS             2827..3150
                     /locus_tag="VIG70_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249797.1"
                     /translation="MIRRIVGLCATMLLASCWSVSSNWYLMDSGYSIVPLQGEQFAVE
                     VHLNQLKQLGGETGSAEFHRFVLERLKWHGICPAGWEPLPCVKDGSCVQRTSRSITVA
                     GRCTS"
     gene            3199..4488
                     /gene="glgC"
                     /locus_tag="VIG70_04265"
     CDS             3199..4488
                     /gene="glgC"
                     /locus_tag="VIG70_04265"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY8249798.1"
                     /translation="MGAGEALSRTISEQAGRWRGGGLARSTYAVILAGGRGSRLKELT
                     DWRCKPAVPFGGKYRIIDFTLSNCVNSGVRRIGVATQYKSHSLIRHLQRGWSFLDGRI
                     GEHVDILPAGQQVAESWYRGTADAVFQNLNILRRDGCKYVLVLSGDHIYKMDYARLLE
                     EHVAGGADATVVCVEVPLADARGFGVVEVDAAARIVGFEEKPAAPRALPGAPGRALAS
                     MGVYVFNAPFLYEQLVRDADDPRSSHDFGKDLIPHVIGRYRVHAHRFAESCVEMPNGE
                     PYWRDVGTIDAYWEANLELAKITPQLNLYDRDWPVWTFQEQLPPAKFVFDDEGRRGMA
                     VDSMISGGCIVSGATVRRSVLFSNVRVDDGALIEDCVLLPDVVVGEGVHLRRAVVDRH
                     CRLQRELLDDPRRFRVTEKGLTLITPEMLGQAVHQLR"
     gene            complement(4485..7151)
                     /locus_tag="VIG70_04270"
     CDS             complement(4485..7151)
                     /locus_tag="VIG70_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEY8249799.1"
                     /translation="MVEHYLRPLLAPRSVALVGATEREGTLGRIVYRNLAAGGLGGAL
                     YPVNPKHREIFGRRAYGRLSELPQPPDLAVIVTPARTVAGIIEDAGAARVRAAVVLTS
                     GFGEAGREGLALQEEVLKAATRGGVRILGPNCLGVMRTDCGLNATFARTPALSGNLAL
                     VSQSGAICGAILDWAHSAQLGFSSVISLGGAIDVDFGEVLEFLAADAASEAILLYVEG
                     IRDARRFVSALRAASRVKPVIALKVGRHASGSRAASSHTGALVGSDAVFDAALRRAGA
                     VRVKTYTQLFAAARVLAGERRAEGERLAILTNGGGPGVIAADSAADNAVPLAVLSETT
                     ISALDKKLPAQWSHGNPIDIIGDAPPERFAAATAAALADPGVDALLAMYAPVAVTAPE
                     DAAHAVADAARGSRKPVLAAWLGDINPNASRAWLEAQGIANFYTPENAVEAFSFLCAH
                     RRNQAQLMQVPAAQRDPDAPLPDLAAAAAIHDLALAQRRELLTEHEAKALLAAFHLPV
                     PKSVIARDRDAAVAAARDIGFPAVIKLHSPDITHKSDVGGVRLNLQSEEMVASAFDDM
                     LRHVRALRPGARIEGVVVQPMLRFAHAREALVGVATDPVFGPVISFGAGGISVEAVRD
                     TAVALPPLNAMLARDLMQRTVLYRLLAGYRNVPPADLDALAAVLTGVSRMVCALPWLS
                     EMDLNPVLTHPGGAVIADARVVIADRSARPRYGHMAIHPYPVDLEGAIRLRDAREVRV
                     RPIRPEDAQREKRFFDRLSERSRYQRFMNHLKELPPRMLARFTQLDYDRELALVALWQ
                     DEFVAVGRYAPNPDGVSAEFALAVADDWQEKGLGHALLERLCDAARAAGYEALVGHIL
                     EANRGMLELAAHLGFVEKERSGREVTLVRRLR"
     gene            7277..7963
                     /gene="rquA"
                     /locus_tag="VIG70_04275"
     CDS             7277..7963
                     /gene="rquA"
                     /locus_tag="VIG70_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodoquinone biosynthesis methyltransferase
                     RquA"
                     /protein_id="HEY8249800.1"
                     /translation="MRSEAIRVPGYLTETYWWAYVHPRAVRVFERQWLVNLILWGNFG
                     KLRDAALDALGERLEGRTLQIACVYGDLTDRLRSRLATGGSLDVVDVLQVQLDNLARK
                     LPGDPRVGLLQGDSSALDIASATYDRALLFFLLHEQPEDVRRRTIAEALRVVKPGGRV
                     VIVDYHRPSLRNPLYLPMVGILKTLEPFAMDLWRNEIPDWFPDAVPIAQLDKKTCFGG
                     LYQLLTLTKG"
     gene            7964..9727
                     /gene="frdA"
                     /locus_tag="VIG70_04280"
     CDS             7964..9727
                     /gene="frdA"
                     /locus_tag="VIG70_04280"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HEY8249801.1"
                     /translation="MQVVQADVVVIGAGGAGLRAAIAAAEREPRLRVALVSKVLPMRS
                     HTVAAEGGAAGVTRAEDSLEQHFNDTVSGGDWLCDQDVVEFFVAHCTAELVRLEHWGC
                     PWSRNPDGHVNTRFFGGMSVQRTWFAADRSGFHMLHTLFQTSLKYPSIRRYDEFFCAE
                     LIVEDGRVQGVVAIEIATGEIVVFECKAVVLATGGAGRVFRQNTNAGTVTGDGMGLAF
                     RAGAPLRDMEFVQWHPTCLPGTGILMTEACRGEGAILVNKDGYRYLQDYGLGPVDPWP
                     RAKAMELGPRDRLSQAFWHELRKGRTVAAPGGPAVHLDLRHLGARKIHERLPLITEAA
                     RTFAGIDAVKQPIPVCPAVHYTMGGILTNVRTETPLRGLYAAGECASVGIHGANRLGS
                     NSLVEIVVFGRVAGESAAEHALSPFPVSSDIQGRAADAAARLASMLNTETGERLAVLR
                     DEMRESMEEGVGIYREAGSLGATCAKLAELRGRYRRGVKLDDRSRAFNTEWLTAIELG
                     FMLEVAEAMAQAAAARRESRGAHMRLDFEARDDANFLKHSLAHHGGEGAPRVEHRPVT
                     ITKSAPKARHYGGEGKKAVLS"
     gene            9724..10449
                     /locus_tag="VIG70_04285"
     CDS             9724..10449
                     /locus_tag="VIG70_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY8249802.1"
                     /translation="MSKTIQIECLRYHPERDAAPRYQTYEVPFTDDMSVLQGLQYIKD
                     RLDGSLTFRWSCRMAICGSCGQMVNGVPVLGCETFLRDYYPGKVRVEPLNHFPIVRDL
                     AIDQTDFVKKLESVKPYLISKNVPEKAAFLQTPRQMEDYYQYSQCINCLLCYAACPQY
                     GLNSAFTGPAALALLHRYNADSRDEGKSERMKVVNAEDGVWGCTLVGYCSEVCPKGVD
                     PARAVNQNKIESTKDYFFRFFRK"
     gene            10446..10829
                     /locus_tag="VIG70_04290"
     CDS             10446..10829
                     /locus_tag="VIG70_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit C"
                     /protein_id="HEY8249803.1"
                     /translation="MKTYTRPMSGWWRRNPFYLWYMLREASCVFITAYALVLLWGLAR
                     LSQGKPQFEAWLASLASPVSIAFHAIALLLVLYHAWTWFKVMPRTMPFVRIGTWRVPD
                     GLIVAFAFASSIVLSGALFIGVWRL"
     gene            10817..11170
                     /gene="frdD"
                     /locus_tag="VIG70_04295"
     CDS             10817..11170
                     /gene="frdD"
                     /locus_tag="VIG70_04295"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HEY8249804.1"
                     /translation="MAALKRSNKPVFWSLFGAGGMLSALIGPMLIYITGIAMPLGIVN
                     FSYEQAAAFAQNGFGKAAILLVISLFLFHGVHRMYHCVHDFGIHVGPGLKAAFHGFAA
                     LGTLAAGWLLLSVGG"
     gene            complement(11202..11924)
                     /locus_tag="VIG70_04300"
     CDS             complement(11202..11924)
                     /locus_tag="VIG70_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8249805.1"
                     /translation="MSTAAQLKREVTCAKCRLRETCLSGDVPAEDLERVENIVYARRR
                     IRRGEALFSAGDPFRCLYAIRTGFFKTSLVDREGREQVTGFFMSGELLGMDGLGDGRC
                     PASAIALEDSDVCAMPYSLIEYIGREVPSLQRRLHSVLAREIVRDHGVMLLLGSMCAE
                     ERLATFLLNLSKRFRRRGYSEAEFHLRMTREEIGSYLGLKLETVSRLFSQFQKDGYIK
                     IAQKLVRIVNIAGLELLLAARN"
     gene            complement(11983..12507)
                     /locus_tag="VIG70_04305"
     CDS             complement(11983..12507)
                     /locus_tag="VIG70_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8249806.1"
                     /translation="MAPAHRLFNRILLPVDGSDHAAHAVEYVIALWRNHPAPATMDIH
                     LMNVQRELPGTASRFVPKESLQDYHRERSEKALERARRMLDEAGVKYAVHLLVGQPWE
                     AISDYAAKHGFDLVVMGTRGLGSYTGSALGSVAHGVAERSSVPVLLVPATQPGAGAAR
                     DVTRGSVSPRQAHP"
     gene            complement(12553..13215)
                     /locus_tag="VIG70_04310"
                     /pseudo
     CDS             complement(12553..13215)
                     /locus_tag="VIG70_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
     gene            13308..13775
                     /locus_tag="VIG70_04315"
     CDS             13308..13775
                     /locus_tag="VIG70_04315"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY8249807.1"
                     /translation="MDIKTPLLLRQDFMGGLAVIAVAAAAFWLARDLPGGNGGGMGPG
                     TLPKGLALLLGLLGLALAIGSLLERGLPLERWSVRGPLLILGALVVFGLAVRPLGLVV
                     AGPLAIVVGAFASSEVRWGETLVFGALMTAFCIGLFKFALGLPIPLAPWLLGY"
     gene            13777..>14169
                     /locus_tag="VIG70_04320"
     CDS             13777..>14169
                     /locus_tag="VIG70_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY8249808.1"
                     /translation="MEFLGHLALGFATALSPMNLLLAFIGCLVGTLIGVLPGVGPIAT
                     IAMLMPLTLKVDPTGALIMLAGIYYGAQYGGSTTAILLNIPGEVTAVVTAIDGHEMAK
                     KGQAGTALGIAALGSFVAGTVATLVIAAI"
BASE COUNT         2183 a         4829 c         4911 g         2246 t
ORIGIN      
        1 tggttctggt gctccaagaa cgtgctggcg cagaatccct tgattcgcag gatctacggc
       61 cgggggcgct tggggtcgaa aacctacaca aggatcatgc gctggaacag ccgggtcggc
      121 gtcacccgga agctcgagcg cctgatcggc ctgcactcgg agtcggtgat ccaggacgtc
      181 gacattccca tttcgcgtgc cgctgagttt ttggcgtttt attcaagaga gattcagttg
      241 tggccgcagt gggtttgtcc catcgggccg cagcgcgatt ccggccgctt cgttctctat
      301 cctgtgaaaa acacctggta tgtcaacttc ggcttctggg acgtaaagcg tacgcgcgag
      361 gcgcattcgc ccggccactt caatcgccag atcgaggaga aggtgagtga gctcggcggc
      421 atcaagtcgc tctactcgga tagctttttt gccgaggcgg atttccaccg ccgctacggc
      481 ggcgcggcct acgacgcgct caagaagaag tacgaccccg ccggcgcttt tccccgcctt
      541 tacgataaat gcgtcctaaa ggcgtgaatt cggggtcagg gtcgcaactc gttttcgcgt
      601 acgcaagaaa cgagttgcga ccctgacccc gaattcctaa aggctatttg aaccgggcgc
      661 gcgggtcgaa gcgcgaggct tcgcgcagct cctggtacag cttttcctcg cgctcggtga
      721 gcgtcgccgg cactgcgatg gtcagcacgg cgtaaaggtc gcccgcgccg ccgcccggct
      781 tcggcaggcc cttgccggcg aggcgcagct tctgtccggc gttcgagccc ggcggcactt
      841 tcatggtgac gcggccttcg agggtcggga tctccacctg cgcgccgagc gcggcttccc
      901 acggcccgag cggcacctcg atttccaggt catggccact cgcgcggtag agcgcgtgcg
      961 gctcgagcgc gatctgcagg tagaggtcgc ccgccgggcc gccgttcagg cccgggccgc
     1021 ccttgccccg cagccgcagc cgctggccgt cggtcgcgcc gcgcgggatg cgcgcagtga
     1081 agctgcggtt gtcgaggtgc acctcgcgct cggtgccgcg gaatgcctcc tccagggtga
     1141 gcttcaccgg cacttcgtag tcttcgccgg cgaaagcccg cttcgagcgg cgctgggcgc
     1201 gcccgaagat ccccatcgac tcgaacagct cgccgaggtc gacgccgtgc aggtcctcgg
     1261 cgcggctgcc ggagccgaag cggtcgaacc agtcgggcgg cgggcggaaa tcctgtccct
     1321 ggcggaagcc gctgccgagg ctgtcgtagg cggcgcgttt ttccttgtcg cgcagcgtct
     1381 cgtaggcctc ggacacctcc tggaacttct ccttggcgtt cgcttccttg gagacgtcgg
     1441 ggtggtactt gcgcgcgagc ttgcggtagg ccttcttgat ctcgtcgtcg ccggcgccgc
     1501 gcgcgacgcc caggatcttg tagtaatcct tgtatttcat aggccgttgt atatgggggc
     1561 ggatcctcag cgctgcaatg acgcgccaac ctgccgccgg atcgcctcca ggaaggcggg
     1621 atggcgcggc aggccgcgct cgtgcaccgt cgggtgcgcg gcccccggca ccacttcgag
     1681 ccgcaacccg ggccagcgtt tcgccatcgc ctgaacgcgc ggcagcgccg ggtccgcgct
     1741 gccgacgacc gcgaggacgg gcacgcgaac cgccgcgatc tcggcgtcgc tcacgagcag
     1801 cgcgcggcgc gcgcgcatga gcgcggcatg cgcccgcacg tcggccgacg cggcgatctc
     1861 gcgcgagcgc gcgcggatcg cgtcctcgcc cggcggcggc tcgtcggtcg gcgcagtcga
     1921 aacgatgagc gcgcggtaca cgccgctctc gatctcgcgc gccgcggcct cggtagcttc
     1981 gtccgcgctc tcgctgcgcg cacgccggta cgccgcgccg ccgagcagcg cgctcgcgaa
     2041 ccgctcgggg tgcgtgacca gcagccgggc ggcgatgatg ccgccgagcg aatagcccac
     2101 gaggtgcgcg cgtgcgaccc ccgcatggtc catcaggcgg atcacgtcga gggcgagctc
     2161 gtcgtaggcg cgcgggtcgt gcggcttgtc gctgtggccg tggccgcgca ggtcgaaggc
     2221 gagcacacgg tgctcgcgcg cgagctcggg tagcaccccg gtgccgatcc acgagcgctc
     2281 aatggtgccg gtgaagccgt gcaccagcac caccggcggc ccggcgccgc ggtcgacgta
     2341 gcggatgcgc acgccgcccg agtcgaagaa ccggtcctgc gccggaacgg ggccggggag
     2401 gaccgacagc aacgcgagta acaggaaagg gcggagcgcc atgggctatg cttgcgccat
     2461 cctaaccacg aaagggaagc cagatggcac gcaagcgaaa gggagccgcg aggaaggcgc
     2521 cggcggccaa caaggcgcag gccacgcggc gcaggctgga agccgaggcg cgcgcgctga
     2581 aaaagcgtct ggacgccctg atggcgagcg cccgcaaggc cgaggtggct gcgcgcgcga
     2641 gcacgatgca gcagatccgg gtgctgcaga agcggcaggc ggcggcgcgg gtcgtgctgg
     2701 ccaagctcgg ccggcagggc gccgcggcga gcgggccgat cctcgtcggt ctgcagaaag
     2761 cctggcgcga catcgacgtc gccgtgcgcg agggcgccaa gcgcttccgc gagacctcgt
     2821 gatcgcgtga tccgccggat cgtcggcctg tgcgccacga tgctgctcgc ctcgtgctgg
     2881 tcggtgagca gcaactggta cctgatggac tccgggtaca gcatcgttcc gctccagggc
     2941 gagcagttcg cggtcgaggt ccacctcaac cagctcaagc agctcggcgg cgaaaccggc
     3001 agcgccgagt ttcaccgctt cgtcctcgag cgcctgaaat ggcatgggat ctgcccggcc
     3061 ggctgggaac ctttgccgtg cgtgaaagac ggctcctgcg tgcagcgcac ctctcgttcg
     3121 atcaccgtcg cggggcgctg cacgagctag ccagccgctc gcattgcgga gtacgatgca
     3181 ggcggcggca gaaacagcat gggcgcaggc gaagcactat cccgcacgat cagcgagcag
     3241 gccgggcgct ggcgcggggg agggctggcg cgcagcacct acgcggtgat tctcgccggc
     3301 ggccgcggca gccgcctcaa ggagctgacc gactggcgct gcaagcccgc ggtcccgttc
     3361 ggcggcaaat accggatcat cgatttcacg ctctccaact gcgtcaactc gggcgtgcgc
     3421 cgcatcgggg tcgccaccca gtacaagtcg cacagcctga tccgccacct gcagcgcggc
     3481 tggagcttcc tcgacggtcg catcggcgag cacgtcgata tcctgcccgc ggggcagcag
     3541 gttgcggaaa gctggtaccg cgggacggcc gacgcggtgt tccagaacct gaacatcctg
     3601 cgccgcgacg gctgcaagta cgtgctggtg ctctcgggcg atcacatcta caagatggac
     3661 tacgcgcggc tgctcgagga gcacgtcgcg ggcggcgccg acgccaccgt ggtgtgcgtc
     3721 gaagtgccgc tggccgatgc gcgaggcttc ggcgtggtcg aggtcgacgc ggcggcgcgc
     3781 atcgtcggct tcgaggagaa gccggccgcg ccgcgtgcgc tgcccggcgc ccccggccgc
     3841 gccctcgcca gcatgggcgt ctatgtattc aacgcgcctt tcctttacga gcagctggtc
     3901 cgcgacgccg acgacccgcg ctcgagccac gatttcggca aagacctgat cccgcacgtc
     3961 atcggccgct accgggtgca tgcgcaccgc ttcgccgaga gctgcgtcga aatgccgaat
     4021 ggcgagccct actggcgcga cgtcggcacg atcgacgcgt actgggaagc gaacctggag
     4081 ctcgccaaga tcacgccgca gctcaatctc tacgaccgcg actggccggt gtggaccttc
     4141 caggagcagc tgcccccggc caagttcgtg ttcgacgacg agggccgccg cggcatggcg
     4201 gtcgactcga tgatctcggg cggctgcatc gtgagcggcg cgaccgtgcg ccgctcggtg
     4261 ctgttctcca acgtgcgcgt ggacgacggc gcgctgatcg aagattgcgt gctgctgccc
     4321 gacgtcgtcg tgggggaggg cgtgcacctg cgccgcgcgg ttgtcgaccg gcactgccgg
     4381 ctgcagcggg aactgctcga tgacccaagg cgcttccggg tcacggagaa ggggctgacg
     4441 ctgatcaccc ccgagatgct cggccaggcg gtgcaccagc tgcgctagcg aagccgccgg
     4501 accagcgtca cctccctgcc cgagcgttcc ttctcgacga agccgagatg ggcggcgagc
     4561 tcgagcatgc cgcgattggc ttcgagaata tggcccacca gcgcctcgta gccggcggcg
     4621 cgcgcggcgt cgcacagccg ctcgagcagc gcgtgcccca gccctttttc ctgccagtcg
     4681 tccgcgaccg cgagcgcgaa ctcggcgctg acgccgtccg ggttcggcgc gtaccggccg
     4741 acggcgacga actcgtcctg ccagagcgcg accagggcga gctcgcggtc gtagtcgagc
     4801 tgggtgaagc gcgcgagcat gcgcgggggc agctccttca ggtggttcat gaagcgctgg
     4861 tagcgcgagc gctcggaaag ccggtcgaag aagcgcttct cgcgctgcgc gtcttccggc
     4921 cggatcggcc gcacgcgtac ctcgcgagcg tcgcgcaggc gaatcgcgcc ctcgagatcg
     4981 accggatagg gatggatcgc catgtgcccg taccgcggcc ttgcgctccg gtcagcgatg
     5041 acgacgcgcg catccgcgat caccgcgccg cccggatgcg tcagcaccgg gttgaggtcc
     5101 atttcgctga gccagggcag agcgcacacc atgcgcgaca cgccggtcag caccgccgca
     5161 agcgcgtcga ggtcggcggg cgggacgttg cgatagccgg cgagcagccg gtacagcacg
     5221 gtgcgctgca tgagatcccg cgccagcatg gcgttgagcg gcggcagggc gaccgcggta
     5281 tcgcgcaccg cttcgaccga gatgccgccg gcgccgaacg agatcaccgg gccgaacacc
     5341 ggatcggtgg cgacgccgac cagcgcttcg cgcgcgtgcg cgaagcgcag catcggctgc
     5401 accacgaccc cttcgatgcg cgcgcccggg cgcagcgcgc gcacgtgccg cagcatgtcg
     5461 tcgaacgccg aggcgaccat ttcctcgctt tgcagattca ggcgcacgcc gccgacgtcc
     5521 gatttgtgag tgatgtccgg cgagtgcagc ttgatcaccg ccgggaagcc gatgtcgcgc
     5581 gccgccgcta ccgctgcgtc ccgatctctc gcaatgacgg atttggggac gggaagatga
     5641 aacgccgcga gcagcgcttt cgcctcgtgc tcggtaagca gctcgcggcg ctgcgccagc
     5701 gcgaggtcgt ggatcgccgc ggccgccgcc aggtccggga gcggcgcgtc gggatcgcgc
     5761 tgcgccgccg gcacctgcat cagctgcgcc tggttgcggc ggtgggcgca aaggaacgag
     5821 aacgcttcca ccgcgttctc cggcgtgtag aagttggcga tgccctgcgc ctcgagccac
     5881 gcgcggctcg cgttcggatt gatgtcgccg agccacgccg cgagcaccgg cttgcggctg
     5941 ccgcgcgctg catcagccac ggcgtgcgct gcgtcctccg gtgcggtcac ggcgaccggc
     6001 gcgtacatcg cgagcagcgc gtcgacgccc ggatcggcaa gcgccgccgc cgtcgccgcg
     6061 gcgaagcgct cgggcggcgc atcgccgatg atgtcgatcg gatttccgtg cgaccactgg
     6121 gcgggaagct tcttatcaag cgctgaaatc gtcgtttctg aaagaacggc taaaggcact
     6181 gcgttgtcgg ccgcgctgtc ggcggcgatc acgcccgggc cgccgccgtt ggtcagtatc
     6241 gcgagccgct cgccttcggc gcgccgctcg cccgcgagca cacgcgccgc ggcgaagagc
     6301 tgggtatagg tcttcacccg caccgccccg gcgcggcgca gcgcggcgtc gaacaccgca
     6361 tcgcttccga ccagcgcgcc ggtgtggctg gacgcggcgc gcgatccgct cgcgtggcgc
     6421 ccgaccttga gcgcgatcac cggcttcacg cgcgaggcgg cgcgcagcgc cgatacaaag
     6481 cggcgcgcgt cgcggatgcc ttcgacgtag agcaggatcg cctcgctcgc cgcgtcggcg
     6541 gcgagaaact cgagcacctc gccgaaatcg acgtcgatcg cgccgccgag cgagatcacg
     6601 ctcgagaagc cgagctgcgc gctgtgcgcc cagtcgagga tcgcgccgca gatcgcgccc
     6661 gactgcgaga ccagcgcgag gtttccggag agcgccggcg tgcgcgcgaa ggtggcgttc
     6721 aggccgcagt cggtgcgcat gacgccgagg caattgggcc cgaggatccg caccccgccc
     6781 cgggttgcgg ccttgaggac ttcctcctga agggccaggc cttcgcggcc cgcttcgccg
     6841 aagccgctcg tcagcaccac cgccgccctt acccgcgccg cgcccgcgtc ctcgatgatt
     6901 cccgccacgg tgcgcgccgg ggtgacgatg acggcgaggt ccggcggctg cggcagctcg
     6961 gacaggcgtc catacgcacg ccgcccgaaa atctcgcggt gcttcggatt gacggggtag
     7021 agcgcgccgc ctagcccgcc ggcggccagg ttgcgataga cgattctgcc aagcgtgcct
     7081 tcgcgctcgg tggcgccgac cagcgcgacg ctgcgcggcg cgagcagcgg ccgcaggtag
     7141 tgctcgacca tgggttcatc catcttacga aaaaacgcag gtttgacctg cgtcaaatgg
     7201 caacgagttt cgttccggac aatccccggc agtaggctag cgtgcattcc atagccgcac
     7261 caagggaggg ctggcgatga ggagcgaagc gatccgggtg ccgggatacc tgaccgagac
     7321 ctattggtgg gcctacgttc atccgcgcgc ggtgcgtgtg ttcgagcggc aatggctggt
     7381 caacctgatc ctgtggggca atttcggaaa gctgcgcgac gctgcgctcg atgcgctcgg
     7441 cgagcggctc gaggggcgca cgctgcagat cgcgtgcgtc tacggcgatc tcaccgaccg
     7501 gctgcgctcg aggctcgcca ccggaggcag cctggacgtg gtcgacgtgc tgcaggtgca
     7561 gctcgacaac ctcgcgcgca agcttcccgg cgatccgcgg gtaggcctgc tccagggcga
     7621 cagctcggcg ctcgacatcg cctcggcgac ctacgaccgg gcgctgctct tcttcctcct
     7681 ccacgaacag ccggaagacg tgcgccggcg cacgatcgcc gaagcgctcc gggtggtgaa
     7741 gcccggcggc cgtgtcgtga tcgtcgacta ccatcgcccg agcctgcgca atccgctgta
     7801 cctcccgatg gtcggcatcc tcaagaccct cgagcccttc gccatggacc tgtggcgcaa
     7861 tgaaatcccc gattggttcc ccgacgcggt tccgatcgcg cagctcgaca agaaaacctg
     7921 cttcggcggc ctgtaccagc tcctcacgct gaccaagggc tagatgcagg tcgtgcaggc
     7981 cgacgtcgtc gtcatcgggg cgggcggcgc tggcctgcgc gctgccatcg ccgccgccga
     8041 gcgcgagccg cgcctgcggg tggcgctggt ttcgaaggtg ctgcccatgc gcagccatac
     8101 cgtggccgcc gaaggcggcg cggcgggcgt gacgcgcgcc gaggacagcc tggagcagca
     8161 cttcaacgac accgtctcgg gcggcgactg gctgtgcgac caggacgtgg tcgagttctt
     8221 cgtcgcgcat tgcaccgcgg agctcgtgcg gctcgagcac tggggctgtc cctggagccg
     8281 caatcccgac ggacacgtca atacccgctt cttcggcggc atgagcgtgc agcgcacctg
     8341 gtttgccgcc gaccgcagcg gctttcacat gctgcacacg ctgttccaga cttcgctcaa
     8401 atacccgtcc atccggcgct acgacgagtt cttctgcgcc gagctgatcg tcgaagacgg
     8461 ccgggtgcaa ggcgtcgtgg cgatcgagat cgccaccggc gaaatcgtgg tgttcgaatg
     8521 caaggccgtg gttctcgcca ccggcggcgc cggccgggtg tttcgccaga acaccaatgc
     8581 cggcaccgtg accggcgacg gcatgggcct cgctttccgc gccggcgcgc cgctgcgcga
     8641 catggaattc gtgcagtggc atccgacctg ccttccggga accggcatcc tgatgacgga
     8701 agcgtgccgc ggcgaaggcg cgatcctcgt caacaaggac ggctaccgct atctgcagga
     8761 ctacggcctc ggaccggtgg atccctggcc gcgcgccaag gcgatggagc tcggcccgcg
     8821 cgaccgcctt tcgcaggctt tctggcacga gctgcgcaag ggccggaccg tggccgcgcc
     8881 cggcggtccg gcggtgcatc tcgatctgcg ccacctcggc gcgcgcaaga ttcacgagcg
     8941 gctgccgctg attacggagg cggcgcgcac ttttgccggc atcgatgcgg tgaagcagcc
     9001 catcccggtc tgcccggcgg tgcactacac gatgggcggc atcctgacca atgtgcgcac
     9061 cgagacgccg ctgcgcggcc tgtacgccgc cggcgagtgc gcgagcgtcg gcatccacgg
     9121 cgccaaccgg ctcggctcca actcgctggt ggagatcgtg gtgttcggac gggtcgcggg
     9181 cgagtccgcg gcggagcatg cgctttctcc cttcccggtg agctcggaca ttcaggggag
     9241 ggccgcggac gccgcggcgc gactggcttc gatgctgaac accgagaccg gcgaaaggct
     9301 cgcggtgctg cgcgacgaga tgcgcgagag catggaggag ggcgtcggga tctaccgcga
     9361 ggccggttcg ctcggcgcga cctgcgcgaa gctcgccgag ctgcgcgggc gctatcgccg
     9421 cggcgtgaag ctggacgacc gcagccgcgc cttcaacacc gagtggctga ccgcgatcga
     9481 gctcggcttc atgctcgaag tggccgaggc gatggcgcag gccgcggctg cccggcggga
     9541 gtcgcgcggc gcgcacatgc gcctggactt cgaggcgcgc gacgacgcga atttcctcaa
     9601 gcattcgctc gcgcaccacg gcggcgaggg cgcaccccgc gtggagcatc ggccggtgac
     9661 gatcaccaag tcggcgccga aggcgcgcca ctacggcggc gagggcaaga aggccgttct
     9721 ctcatgagca agacgatcca gatcgagtgc ctgcgctatc acccggagcg cgacgccgcg
     9781 ccgcgctacc agacctacga ggtgccgttc accgacgaca tgtcggtgct gcaggggctg
     9841 cagtacatca aggaccgcct cgacggcagc ctgaccttcc gctggtcctg ccgcatggcg
     9901 atctgcggga gctgcggcca gatggtgaac ggcgtgccgg tgctcggctg cgagacgttc
     9961 ctgcgcgact actatcccgg aaaggtccgc gtcgagcccc tcaaccactt tcccatcgtg
    10021 cgcgatctcg cgatcgatca gaccgacttc gtcaagaagc tggagtcggt gaagccatat
    10081 ttgatttcga agaacgtccc ggaaaaagcc gccttcctgc agacgccgcg gcagatggag
    10141 gactattacc agtactcgca gtgcatcaac tgcctgctct gctacgccgc ctgcccgcag
    10201 tacggcctca actcggcgtt caccgggccg gcggcgctcg cgctcctgca ccgctacaac
    10261 gccgactcgc gcgacgaagg caagagcgag cgcatgaaag tcgtcaatgc cgaggacggc
    10321 gtctggggct gcacgctggt gggctactgc tccgaggtct gccccaaggg agtggacccg
    10381 gcgcgcgccg tcaaccagaa caagatcgag agcaccaagg actacttctt caggtttttc
    10441 cgcaaatgaa aacctatacg cgcccgatgt cggggtggtg gcgccgcaac cccttctacc
    10501 tctggtacat gctgcgcgaa gcgagctgcg tgttcatcac ggcttatgcg ctggttctgc
    10561 tttggggtct cgcaagactc tctcaaggta aaccgcagtt cgaggcgtgg cttgcgagcc
    10621 tggcaagccc ggtttcgatc gcctttcacg caatcgcgct gctcctcgtg ctgtatcacg
    10681 cctggacctg gttcaaggtg atgccgagaa ccatgccgtt cgtgcgcatc ggcacctggc
    10741 gcgtgccgga cgggttgatc gtcgcgttcg cctttgccag ctcgatcgtg ctgtcgggcg
    10801 cactcttcat cggcgtatgg cggctctgaa gcgttccaac aagcccgtct tctggtccct
    10861 gttcggcgcc ggcgggatgc tgtccgcgct gatcggcccg atgctcatct acatcaccgg
    10921 catcgccatg ccgctcggta tcgtcaactt cagctatgag caggcggccg cgttcgcgca
    10981 gaacggcttc ggcaaggctg cgatcctcct ggtcatctcg ctgttcctgt tccatggggt
    11041 gcaccgcatg taccactgcg tgcacgactt cggcatccac gtcggccccg gcctgaaggc
    11101 ggccttccac ggcttcgctg ccctcggcac gcttgccgca ggttggctgc tgctctccgt
    11161 cggcgggtaa acggcggaat tagccgcgtc cggtgcgggc atcaattcct ggccgcgagc
    11221 aagagctcga ggccggcgat gttgacgatg cgcacgagct tctgcgcgat cttgatgtag
    11281 ccgtccttct ggaactggga gaacaggcgg ctcacggtct cgagcttgag gccgagatag
    11341 ctaccgatct cctcgcgtgt catgcgcagg tggaactccg cctcggagta gccgcgccgc
    11401 cggaagcgct tcgagagatt gaggaggaag gtggcgaggc gctcctccgc gcacatcgag
    11461 cccagcagca gcatgacgcc atggtcgcgc acgatctcgc gcgccagcac cgaatgcagg
    11521 cggcgctgca gcgacgggac ttcccggccg atatactcga tcagcgagta cggcatcgcg
    11581 cagacgtcgc tgtcttcgag cgcgattgcg cttgccgggc agcggccgtc gcccaggccg
    11641 tccatgccga gcagctcccc gctcatgaag aacccggtga cctgctcgcg gccttcgcgg
    11701 tcgacgaggc tggtcttgaa gaagccggtg cggatggcat agaggcatct gaacgggtcg
    11761 cccgcgctga acagggcttc accgcgcctg atccggcggc gcgcgtagac gatgttctcg
    11821 acgcgctcga gatcttcggc gggcacgtcc ccggagaggc acgtttcccg gagcctgcat
    11881 tttgcgcagg tgacttcgcg cttgagctgt gcggctgtgg acatgcccgg ccgcatgatc
    11941 cgggcgcgtc gccgggccat ctgttcggta acgcacacaa ggtcaagggt gcgcttgccg
    12001 cggcgaaacg ctgccccggg tcacgtcgcg agcagcaccg gcgccgggct gcgtggcggg
    12061 gaccagcagg accggcaccg agctgcgctc ggccacgccg tgggcgaccg agccgagcgc
    12121 cgagccggtg taggacccga gcccgcgcgt gcccatgacg acgaggtcga agccgtgttt
    12181 cgcggcgtag tcgctgatcg cctcccacgg ctggccgacc agcaggtgga cggcgtactt
    12241 gacgcccgcc tcgtcgagca tccggcgcgc gcgctcgagc gccttctcgc tgcgctcgcg
    12301 gtgatagtcc tgcagcgact ccttcgggac gaagcggctc gccgtgccgg gcagctcgcg
    12361 ttgcacgttc atgaggtgga tgtccatggt tgcgggcgcg ggatggttgc gccacaacgc
    12421 gatcacgtac tccaccgcgt gcgcggcgtg gtccgaaccg tccaccggca gcaggatgcg
    12481 attgaagagg cgatgcgccg gggccacggc gggagccgga gcggccgctg ccgctgccgc
    12541 gcgccgtgta cgcgtccggc gggcgagcca ggtgccggcc aggacgaccg acacggcgcc
    12601 cgcggccgag gcggcccagt gcagcagcgc ggcgttcgca tcgatccatt ccttgaccag
    12661 cgggtcggtg acgaacatct ctcccgcgac ccacccgagc agcgcggcgc cgatggtgat
    12721 gatgaccggg aagcgctcca tcagcctgag gagaagcgtg ctgccgaaga ccaccagcgg
    12781 gatgctgatc gcgaggccga gaatgagcag caggaggctg cccttcgccg cggcggccac
    12841 ggcgatgacg ttgtcgaggc tcatcaccag gtctgcgatg aggatcgtcc tcatcgcgcc
    12901 ccagatgccg atgacgctgc cagattgcgc gccgtgcccg ccctcgggct gaagcagctt
    12961 caccgcgatc cccaggagaa gcacaccgcc ggcaatcttc agcagaggca accggagcag
    13021 ctcgaccgcg actatggtca gcacgatccg catcagcacc gcggcgccgc tgccccagac
    13081 gatcgcctgg cgctgctgct tcgccggcag ggagcgcgcg gcgagcgcga tgaccacggc
    13141 attgtcgccc gacaggacga ggttgatcag gatgatctgt ccgagcccgg tccagaaccg
    13201 ggggtcgccg agcatttcgg gcatgcgggg cagcatggcg gcgcatgcaa cgcgagattt
    13261 gacacagatc aacctcgccg cccgaaacga cagaagaatg gcccgcagtg gatatcaaga
    13321 cccctctgct gctcaggcag gacttcatgg gcgggctcgc ggtgattgcg gtcgcggcgg
    13381 ctgccttctg gctggcgcgc gatctgcccg gcggcaacgg cggcggcatg ggcccgggaa
    13441 cgctgccgaa gggcttggcg ctgctgctcg gcctattggg ccttgccttg gcaatcggtt
    13501 ccttgctcga aaggggattg ccgctcgagc gctggtcggt gcgcggcccg ctgctgatcc
    13561 tgggcgcgct ggtggtgttc ggcctcgcgg tgcgcccgct cggcctcgtg gtcgcgggcc
    13621 cgctcgcgat cgtcgtgggc gcattcgcca gcagcgaagt gcgctggggc gaaacgctgg
    13681 tgttcggagc gctgatgacg gcgttctgca tcgggctttt caagttcgcc ctcgggctcc
    13741 ccattccgct ggcgccctgg ctgctcggct actgagatgg agttcctcgg ccacctcgcc
    13801 ctcggcttcg ccaccgcgct ctcgccgatg aatctgctgc tcgccttcat cggctgcctg
    13861 gtcgggacgc tgatcggcgt gctgcccggc gtcgggccga tcgcgaccat cgccatgctg
    13921 atgccgctca cgctgaaggt ggacccgacg ggcgcgctga tcatgctcgc gggcatctat
    13981 tacggcgcgc aatacggcgg ctcgaccacc gcaatcctgc tcaacatccc cggcgaggtg
    14041 accgcggtgg tcacggccat cgacggccac gaaatggcga agaagggcca ggcgggcacc
    14101 gcgctcggca tcgccgcgct cggctcgttc gtcgccggca cggtggccac gctcgtcatc
    14161 gccgcgatc
//
LOCUS       DATBSZ010000066        10279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1375507, whole genome shotgun sequence.
ACCESSION   DATBSZ010000066 DATBSZ010000000
VERSION     DATBSZ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10279
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375507"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1237
                     /locus_tag="VIG70_04325"
     CDS             47..1237
                     /locus_tag="VIG70_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY8249809.1"
                     /translation="MKRENVKAGFVWDDPLLLEQQLTQDERMVRDAANAYCQKKLAPR
                     VLEAFRQEKTDPAIFREMGELGLLGPTIPAEYGGPGLNYVSYGLIAREVERVDSGYRS
                     MMSVQSSLVMVPIFEFGSAATKKKYLPKLATGEWIGCFGLTEPNHGSDPGSMQSRAKQ
                     VAGGYSLTGSKTWISNSPIADVFVVWAKDEAGDIRGFVLEKSFKNLSAPAIHGKVGLR
                     ASITGEIVMDGVFCPEENAFPEVRGLKGPFTCLNSARYGIAWGALGAAEDCWHRARTY
                     VMDRKQFGRPLAANQLIQKKLADMQTEIALGLQGCLRLGRMKDEGSASPEITSILKRN
                     SCGKALDIARLARDMLGGNGISDEFGVIRHLVNLEVVNTYEGTHDIHALILGRAMTGI
                     QAFN"
     gene            1315..1785
                     /locus_tag="VIG70_04330"
     CDS             1315..1785
                     /locus_tag="VIG70_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY8249810.1"
                     /translation="MLIAKPSLTDPNFRETVVLVTQTTDSSTVGVILNRPTPNKHEPT
                     DAPLFFGGPVMREVLVALYRSEGAPEAAAFHVLKGVYLTMHPRNLEKLWGNDRERYRL
                     FMGFAGWAPGQLESELARDDWFVLPASADIVFRRDTRGLWEELVRKARGRVAMR"
     gene            1806..2420
                     /locus_tag="VIG70_04335"
     CDS             1806..2420
                     /locus_tag="VIG70_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY8249811.1"
                     /translation="MNPVFRVLAFLGLLVSSASAHADGDEAMILIAHPAFRDLEYRQT
                     VLIAAPAPNGGHVGVILNRPTRRSLGSLFPEHEPSKKVMDPVYYGGPFSRGALVALVR
                     ADRAPGAGSVLLMSNLYLAFRANTIDHVIETTPNEARYFVGYVGWRPGELKSEIDRGL
                     WSVTGAELDTVFRKDTEGLWEELLQQSKRIRATLPLDEGFALLR"
     gene            2485..2805
                     /locus_tag="VIG70_04340"
     CDS             2485..2805
                     /locus_tag="VIG70_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEY8249812.1"
                     /translation="MFRLSKIEWLVLAGALLLCPGFSFGQAGAGATAPAASLFDRLDR
                     NKDGYLSRGELGSDEAKTRNWIAVDRDRDGRISRAEFGLVNIPSAAPQPSAAAGGTAP
                     PKQE"
     gene            2876..4441
                     /locus_tag="VIG70_04345"
     CDS             2876..4441
                     /locus_tag="VIG70_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8249813.1"
                     /translation="MKQALIALCSAVLLVSCATPTREQSLVSRAVDAMGGADRIAGVK
                     TITFKGTSKYWEPEQSDAPGGESRFANESSFEGFIDAASRSSRTDIVRNFAYPAPRTF
                     TFSEVVTPEAGYVLGVDSNGRNAQNLKSTPPGHSMSGFRLATTQREARRAGTGQLMLA
                     MQRSPDKVQPAADINGQPAVSFDGFIVAFDPATGLPSRVRTLDYDNIWGDVNYDMILS
                     DWRDLGGYKVPMNRKYELAGRPVVETNLTDVRFNQSVDTARLQVPADLRTGAAKPAGR
                     NVPYQWVMRRQFIGTYLDSDNVSYDTRGSQGLRLQEIAPGVHQIQGGSHNSLVVEMSD
                     HLIVLDAPVTDAQSMWLVGATRAQFPGKPIRWVVLTHHHMDHAGGVRGLLAEGGTLVV
                     GQGAGAHFRRVLSAPMTRNPDLPSRSFHMNPILEVPESHVMSDASGRQVIVYVMDNPH
                     AKGMLMGWVPHARLGYVTDIWTPGPPLPPKPNPGLISVVNTVKRAGLQPERFAGGHGS
                     TADYQSLTRLVGP"
     gene            complement(4556..7111)
                     /gene="clpB"
                     /locus_tag="VIG70_04350"
     CDS             complement(4556..7111)
                     /gene="clpB"
                     /locus_tag="VIG70_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY8249814.1"
                     /translation="MRFEKFTTKLQEALATAQSNALGQDHQYIEPQHLLLALLEDQGI
                     SGLIAKAGGNPGVLKKSLSESVARLPKVEGTPGEVHLSRELAQLLNVADKEAQKRGDQ
                     YIASELFLLAAVKLLGVPKPALEKAIQEVRGGESVSSPAGEEQRQALAKYTLDLTERA
                     KAGKLDPVIGRDDEIRRCIQILQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPE
                     SLKNKRVLVLDMASLLAGAKYRGEFEERLKSVLKELAKDEGNTIVFIDELHTMVGAGK
                     AEGAIDAGNMLKPALARGELHCIGATTLNEYRKYVEKDAALERRFQKVLVGEPSVEAT
                     IAILRGLQEKYEVHHGVEITDPAIVAAAELSNRYITDRFLPDKAIDLIDEAAARIKME
                     IDSKPEALDRLDRRLIQLRIEREAVKKEKDEASQKRLKLIEEEIGRLEKEAADLEEEW
                     KAEKATVAGSQHVKEELEKLKLQMEEAKRKGDWQKMSEIQYGKIPPLEAQLKNSSLPA
                     KTRLLRTQVGAEEIAEVVSRATGIPVSKMMQGEREKLIKMEERLHERVVGQDEAVRLV
                     ADAIRRSRSGLSDPNRPYGSFLFLGPTGVGKTELCKALAMFLFDAEDHLIRIDMSEYM
                     EKHSVSRLIGAPPGYVGYDEGGQLTEQVRRKPYSVILFDEIEKAHPDVFNALLQVLDE
                     GRLTDGQGRTVDFRNTVVVMTSNLGSQMIQSMAGSDYQVVKLAVMGEVKTQFRPEFVN
                     RIDEIVVFHALGEEHIKAIARIQLKILETRLAKMDYGLEISEAAVAELAKVGFDPVYG
                     ARPLKRAIQSELENPLAKAILEGRFAPKDTIAADWRGGKMVFEKKKATARRAA"
     gene            7203..9017
                     /gene="ftsH"
                     /locus_tag="VIG70_04355"
     CDS             7203..9017
                     /gene="ftsH"
                     /locus_tag="VIG70_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY8249815.1"
                     /translation="MERRTQFNIGYLLFALVAILVVQQWWQQAQTIEVVPYSEFEKLL
                     AEDRIDEVMVSDQRITGKLKSPEKNGKTVAVANLVQPDLAERLSKFGVKYTRVHESTF
                     LRDLLSWVLPALVFFGVWFLLARRMANQAGGLGGFMSIGKSRAKVYVENKTGVTFDDV
                     AGVDEAKDELREVVEFLKEPKKYGRLGARVPKGVLLVGPPGTGKTLLARAVAGEAGVP
                     FFSISGSEFVEMFVGVGAARVRDLFEQARQKAPAIIFIDELDALGRARGAGGFGGHDE
                     KEQTLNQLLVELDGFDPSTGLVLLAATNRPEILDPALLRAGRFDRQVLVDRPDKPGRI
                     QILRVHSKKIKLQHGVDLEQVAALTPGFTGADLANLVNEAALLATRRGAESVSLADFT
                     QAIERIVAGLEKKNRLLNPKERRIVAHHELGHAMVAMALPGTDPVHKVSIIPRGVGAL
                     GYTIQRPIEDRFLMTRGELENKMAVLLGGRAAEDVVFGHLSTGAADDLAKVTDIARSM
                     VMRYGMVKSLGHVAYDEERSPFLAGQIIPKSREYSEETAREIDVAVRDIVKACYEKAR
                     GILSREKALLESWALKLLEKETLAEAELAELRASISPA"
     gene            complement(9019..>10279)
                     /locus_tag="VIG70_04360"
     CDS             complement(9019..>10279)
                     /locus_tag="VIG70_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEY8249816.1"
                     /translation="DRHYATIPLPEAMDLVRGALGAQGEGTERHVHSPGPEQRLNVVL
                     VSVESLGAEFLGRYGNPDGLTPHLDRLASESLWFSRVYATGNRTVRGMEALSLALPPT
                     PGQSIVRRPNNDTLFSLGSVFEDKGYAVVFAYGGYGYFDNMNAFFDANDYRSVDRRAI
                     PSARVQHETVWGVADEHLFDQVLDEIDREHKTRPERPFFVHVMTTSNHRPYTYPAGRI
                     DIPSGSGRGGAVKYTDYAIGRFLAAARAKPWFDDTLFVITADHGANARGTTRIPVDKY
                     LIPVFVYAPKHVAPRRIDRLMSQIDIPPTVLGLLDFDYYSKFFGRDVLRSPVESDRAF
                     VANYQTLGYLARDRMALLHPRRKTEAFRVDAEKNILQQIDDPQLVREAIAFYSTASFV
                     FRNGLYRDEEQTPPEQRAALVRGRPGG"
BASE COUNT         1692 a         3460 c         3374 g         1753 t
ORIGIN      
        1 gcatcggcgt cggccagggc ctcgccgtca tcctggagcg catgcaatga aaagagagaa
       61 cgtgaaagcg ggcttcgtgt gggacgaccc gctgctgctc gagcagcagc tcacccagga
      121 cgagcgcatg gtgcgcgacg cggcgaacgc gtactgccag aaaaagctcg caccccgggt
      181 gctggaagcc ttcaggcagg aaaagaccga cccggcgatc tttcgcgaga tgggcgagct
      241 cgggctgctc gggccgacga tccccgcgga atacggcggg ccggggctca actacgtctc
      301 ctacggcctg atcgcgcgcg aggtggagcg cgtcgactcg ggataccgct cgatgatgag
      361 cgtgcagtct tcgctcgtga tggtgccgat ctttgaattc ggcagcgcgg ccacgaagaa
      421 aaaatacctg ccaaaactcg ccaccggcga gtggatcggc tgcttcggcc tcaccgagcc
      481 gaaccacggc tcggatcccg gctcgatgca atcgcgcgcg aaacaggtcg ccggcggcta
      541 cagcctcacc ggaagcaaga cctggatctc gaattcgccc atcgccgacg tgttcgtggt
      601 gtgggcgaaa gacgaggcgg gcgacatccg tggatttgtt ctggagaaaa gcttcaagaa
      661 tctctcggca ccggcgattc acggcaaggt cggcctgcgc gcctcgatca ccggcgagat
      721 cgtgatggac ggcgtgttct gcccggagga gaacgcgttt cccgaggtgc gcggcctgaa
      781 ggggccgttc acctgcctca actcggcgcg ctacggcatc gcctggggcg cgctcggcgc
      841 ggccgaggat tgctggcacc gcgcgagaac ctatgtcatg gaccggaagc agttcggccg
      901 gccgctcgcc gccaaccagc tgatccagaa gaagctcgcc gacatgcaga ccgagatcgc
      961 gctcggcctg cagggctgcc tgcgcctggg acgcatgaag gacgaaggca gcgcttcgcc
     1021 ggaaatcacg tccatcctga aaagaaactc ctgcggcaag gcgctcgaca tcgcgcgcct
     1081 cgcgcgcgac atgctcggcg gcaacggaat ttcggacgag ttcggcgtca tccggcacct
     1141 ggtcaatctc gaggtggtga acacctacga gggcacgcac gacatccacg cgctgatcct
     1201 ggggcgcgcc atgaccggaa tccaggcatt caactgaagc cctggctctc ggcgctgctg
     1261 gtcgcggcgg cgccggtcct cgcgcaaccg ctggccgagc cgccgaacgg gctcttgctg
     1321 atcgcgaagc ccagcctcac cgatccgaat ttccgcgaga ccgtggtgct cgtgacccag
     1381 accacggact cgagcaccgt cggcgtgatc ctcaaccggc cgacgccgaa caagcacgag
     1441 ccgacggatg cgccgctttt cttcggcggc ccggtgatgc gcgaagtgct ggtcgcgctc
     1501 tatcgctcgg aaggcgcgcc cgaggccgca gcgtttcatg tcctcaaggg cgtctatctc
     1561 accatgcacc cgcgcaacct ggagaaattg tggggaaatg acagggaacg ctatcgcttg
     1621 ttcatgggct tcgccggctg ggcgccgggc cagctcgaga gcgagctcgc gcgcgacgac
     1681 tggttcgtgc tgccggcgag cgcggacatc gtgtttcgca gggacacgcg cgggctgtgg
     1741 gaagagctcg tgagaaaagc ccgcggccgt gtagcgatgc gctgaaagga aaagtatact
     1801 ggcgcatgaa ccccgttttc cgcgtgctgg cgttcctcgg gctgctcgtg agctccgcct
     1861 cggcgcacgc cgatggcgac gaagcgatga tcctcatcgc ccatccggca ttccgcgacc
     1921 tcgagtaccg gcagacggtc ctgatcgccg ccccggcgcc gaacggcggc cacgtcggcg
     1981 tgatcttgaa ccgcccgacg cggcgctcgc tcgggagcct gttccccgag catgagccct
     2041 ccaagaaggt aatggacccg gtctattacg gcggcccgtt ctcgcgcggc gcgctggtgg
     2101 cgctggtcag ggcggaccgg gcgccgggcg ccgggtccgt gctgctgatg agcaacctct
     2161 acctggcgtt ccgcgccaac accatcgacc acgtgatcga gaccacgccg aacgaggcgc
     2221 gttatttcgt gggctacgtc ggctggcgtc cgggcgagct gaagagcgag atcgaccggg
     2281 ggctgtggtc agtgaccggc gccgagctcg acactgtctt ccgcaaggac accgaaggcc
     2341 tctgggagga gctgctgcag cagtccaagc gcatccgcgc caccctgccg ctcgacgagg
     2401 gcttcgccct cctgcgctga ccggctcacg cccggtcttt ttcccgatgt aagtagttga
     2461 agcttcgcgg gctattctgg ccgcatgttc aggctgtcga agatcgaatg gctggtcctt
     2521 gccggagcgc ttctcctgtg cccggggttt tcattcggcc aagcgggcgc cggcgccacg
     2581 gcgcccgcgg cgtcgctctt cgatcgcctc gaccgtaaca aggacggcta cctcagccgc
     2641 ggggagctgg gaagcgacga ggcgaagacc cgcaactgga tcgccgtcga ccgcgaccgc
     2701 gacggccgca tcagccgggc cgaattcggc ctcgtcaaca tcccgtcggc cgcaccgcag
     2761 ccgagcgccg ccgccggcgg caccgccccc ccgaaacagg aatagtgaag caggaatagt
     2821 tgtagtcgcg gtcctgttcg cctaggctgg tcgacccatc ctgggaggag cattcatgaa
     2881 acaggccctc atcgcgctgt gcagcgccgt ccttcttgtt tcctgcgcca ccccgactcg
     2941 agaacagagc ctcgtcagcc gcgccgtcga cgccatgggc ggcgccgacc gcatcgccgg
     3001 ggtcaaaacc attaccttca agggaacttc caagtactgg gagccggagc agtccgacgc
     3061 cccgggcggc gagtcgcgct tcgccaacga atccagcttc gaggggttca tcgacgcggc
     3121 gagccgctcc agccgcaccg acatcgtcag gaacttcgcc tatcccgcgc cgcggacgtt
     3181 caccttcagc gaggtcgtca cgcccgaggc cggttatgtg cttggcgtgg acagcaacgg
     3241 ccgcaatgcg cagaacctga aatccacccc gcccgggcat tcgatgtcgg gcttcaggct
     3301 cgcgacgacg cagcgcgaag cgcgccgcgc cggcaccggc cagttgatgc tcgcgatgca
     3361 gcgcagcccg gacaaggtgc agcccgcggc cgacatcaac ggccaacccg cggtttcctt
     3421 cgacggcttc attgtcgcgt tcgaccctgc caccggcctg ccttcgcgcg tgcgcacgct
     3481 cgactacgac aacatctggg gcgacgtgaa ctacgacatg atcctgtcgg actggcgcga
     3541 ccttggcggg tacaaggtcc cgatgaaccg caagtatgag ctcgccggca ggccggtcgt
     3601 cgaaacgaac ctgacggatg tccgcttcaa ccagtcggtc gacaccgcgc gcttgcaggt
     3661 cccggcggac ttgcgaaccg gcgcggccaa gcccgccggg cgcaacgtgc cttaccagtg
     3721 ggtgatgcgg cgccagttca tcggcaccta cctggattcg gacaacgtga gctacgacac
     3781 gcgcggctcg caggggctgc ggctgcagga aatcgcgccg ggcgtccacc agatccaggg
     3841 cgggtcgcac aacagcctgg tcgtcgagat gagcgatcac ctgatcgtgc tcgatgcgcc
     3901 ggtgaccgac gcgcagtcga tgtggctggt cggcgcgacg cgcgcgcaat ttcccggcaa
     3961 gccgatccgc tgggtcgtgc tcacgcacca tcacatggac cacgccggcg gcgtgcgcgg
     4021 cctcctcgcc gaaggcggca cgctggtcgt cggccagggc gcgggggcgc atttccgccg
     4081 cgtgctctcg gcgccgatga cgcgcaatcc cgacctgccg tcgcgctcgt tccacatgaa
     4141 cccgatcctg gaagtgccgg agagccacgt catgtccgac gcgagcggcc gtcaggtcat
     4201 cgtgtatgtc atggacaacc cgcatgccaa gggcatgctc atgggctggg tgccacacgc
     4261 gaggctcggg tacgtaacgg acatctggac gcccgggccg ccgctccccc cgaagccgaa
     4321 tcccgggctc atctcggtgg tcaacaccgt gaagcgcgcc gggctgcagc ccgagcgctt
     4381 cgccggcggc cacggctcga ccgcggacta ccagtcgctt acgcggctag taggcccgta
     4441 aaagttcggg gacggagccc taactccgtg ggggacggag ccctaactcc gaaagctgcg
     4501 cagccgtgga gttagggctc cgtccccgaa ttgtttaggg ctccgtcccc gaactctacg
     4561 cggctcgtcg ggccgtagcc ttcttcttct cgaacaccat cttgccgccg cgccagtcgg
     4621 cggcgatggt gtccttgggc gcgaagcgcc cctcgaggat cgccttcgcc agcgggtttt
     4681 cgagctcgga ctggatcgcg cgcttgagcg ggcgcgcgcc atacaccggg tcgaagccga
     4741 ccttcgccag ctcggccacc gcggcctccg agatttccag gccgtagtcc atcttcgcga
     4801 ggcgggtctc gagaatcttc agctggatcc gcgcgatcgc cttgatgtgc tcctcgccga
     4861 gcgcgtggaa caccacgatc tcgtcgatgc ggttcacgaa ctccggccgg aactgcgtct
     4921 tgacctcgcc catcaccgcg agcttcacca cctgatagtc gctgccggcc atgctctgga
     4981 tcatctgcga gccgaggttc gaggtcatca ccaccacggt gttcctgaaa tccacggtgc
     5041 ggccctggcc gtcggtgagg cgcccctcgt cgagcacctg gaggagcgcg ttgaacacgt
     5101 cggggtgcgc cttctcgatc tcgtcgaaca ggatcaccga atagggcttg cgccgcacct
     5161 gctcggtgag ctgcccgccc tcgtcgtagc cgacatagcc cggcggcgcg ccgatcagcc
     5221 ggctcaccga gtgcttctcc atgtactcgg acatgtcgat gcggatgaga tggtcctcgg
     5281 cgtcgaacag gaacatggcg agcgccttgc agagctcggt cttgcccacg cccgtcgggc
     5341 cgaggaacag gaagctccca tacggacggt tgggatccga aagacctgag cgcgagcgcc
     5401 ggatcgcgtc ggccacgagg cgcaccgcct cgtcctggcc gaccacgcgc tcgtgcagcc
     5461 gctcttccat cttgattagc ttctcgcgct cgccctgcat catcttcgac accgggatgc
     5521 cggtcgcgcg cgacaccacc tcggcgatct cctcggcgcc gacctgggtg cgcagcagcc
     5581 tcgtttttgc aggaagagaa gaattcttga gctgcgcttc gagcggcgga atcttgccgt
     5641 actggatctc cgacatcttc tgccagtcgc ccttgcgctt cgcctcttcc atctggagct
     5701 tgagcttctc cagctcttcc ttgacgtgct ggctgcccgc gaccgtcgcc ttctcggcct
     5761 tccactcttc ctcgaggtcg gcggcttcct tctccagccg gccgatttcc tcttcgatca
     5821 gcttcaacct tttttgcgaa gcctcgtcct tctccttctt gaccgcctcc cgctcgatgc
     5881 gcagctggat caggcggcga tcgaggcggt cgagcgcctc gggcttggag tcgatctcca
     5941 tcttgatgcg cgccgccgct tcgtcgatca ggtcgatcgc cttgtcgggc aggaagcggt
     6001 cggtgatgta gcggttggaa agctccgccg cggcgacgat cgccgggtcg gtgatctcga
     6061 cgccgtggtg cacttcgtac ttctcctgca ggccgcgcag gatggcgatg gtcgcctcga
     6121 cgctcggctc gccgaccagc actttctgga agcggcgctc gagcgccgca tccttctcga
     6181 catacttgcg gtactcgttg agcgtggtcg cgccgatgca gtgcagctcg ccccgcgcca
     6241 gcgcgggctt gagcatgttg ccggcgtcga tcgcgccttc ggccttgccc gcgcccacca
     6301 tcgtgtgcag ctcgtcgatg aagacgatgg tatttccctc gtccttcgcc agctccttca
     6361 gcaccgactt cagccgctcc tcgaactcgc cgcggtactt cgcgccggcg agcagcgacg
     6421 ccatgtcgag caccagcacg cgcttgttct tcagcgactc gggcacctcg ccgttcacga
     6481 tcctttgcgc gaggccttcc acgatcgcgg tcttgcccac ccccggctcg ccgatcagca
     6541 ccgggttgtt cttggtgcgc cgctgcagga tctggatgca gcggcggatc tcgtcgtcgc
     6601 gcccgatgac cgggtcgagc ttgccggctt tcgcgcgctc ggtgaggtcg agggtgtatt
     6661 tggcgagggc ctgcctttgc tcttccccgg ccggggaaga aacgctttcg ccgccgcgca
     6721 cttcctggat cgctttctcc agggcgggct tcggcacgcc gagaagcttc accgcggcga
     6781 gcaggaacag ctccgaggcg atgtactggt cgccgcgctt ctgcgcctcc ttgtcggcga
     6841 cgttgaggag ctgcgcgagc tcgcgcgaga gatgcacctc gcccggcgtg ccttccacct
     6901 tcggcagccg tgccaccgac tccgataggc tctttttcag gacaccagga tttccgccgg
     6961 ccttggcaat gaggcccgaa atgccctggt cctcgagcag cgccagcagc aggtgctggg
     7021 gctcgatgta ctggtggtcc tgcccgagcg cgttgctctg cgccgtggcc agggcctcct
     7081 gcagcttggt ggtgaatttc tcgaatctca tataacccct tgggagcaga tggggattgg
     7141 accgcgcctt tcaagtctga ttcttgcttt gaaaatgggg ttttagcccc caaattccgg
     7201 agatggaacg ccgcacgcag ttcaacatag gctacctgct gttcgccctg gtcgcgatcc
     7261 tcgtcgtgca gcagtggtgg cagcaggcgc agaccatcga ggtcgttccg tacagcgagt
     7321 tcgagaagct gctggccgag gaccgcatcg acgaggtgat ggtctccgac cagcgcatca
     7381 ccggcaagct caagtccccg gaaaagaacg gcaagacggt cgcggtggcg aacctggtgc
     7441 agcccgacct cgccgagcgc ctctccaaat tcggggtcaa gtacacgcgc gtgcacgaga
     7501 gcaccttcct gcgcgacctc ctttcgtggg tgctgccggc gctggtgttc ttcggcgtct
     7561 ggttcctgct ggctcgccgc atggccaacc aggccggcgg gctgggcggc ttcatgagca
     7621 tcggcaagag ccgcgccaag gtctatgtcg agaacaagac cggtgtcacc ttcgacgacg
     7681 tggcgggcgt cgacgaggcc aaggacgagc tgcgcgaggt ggtcgagttc ctgaaggagc
     7741 cgaagaaata cgggcgcctc ggcgcccgcg ttcccaaggg cgtgctgctg gtcgggccgc
     7801 cgggcaccgg caaaacgctg ctcgcgcgtg cggtcgccgg cgaagcgggc gtgccgttct
     7861 tctcgatctc gggctccgag ttcgtcgaga tgttcgtcgg cgtcggcgcc gcgagagtca
     7921 gggacctgtt cgagcaggcg cggcagaaag ccccggcgat catcttcatc gacgagctcg
     7981 acgcgctcgg ccgcgcgcgc ggcgccggcg gcttcggcgg gcacgacgag aaggagcaaa
     8041 ccctcaacca gttgctggtc gagctcgacg gcttcgaccc gagcaccggc ctggtgctgc
     8101 tggcggcgac caaccggccg gaaatcctcg atccggcgct gctgcgcgcc gggcgcttcg
     8161 accgccaggt gctggtcgac cggccggaca agcctgggcg catccagatc cttcgggttc
     8221 attccaagaa gataaagctc cagcacggcg tcgacctcga acaggtcgcg gcactgacgc
     8281 cgggcttcac cggcgccgac ctcgccaacc tggtcaacga ggcggcactg ctcgccacgc
     8341 ggcgcggcgc cgagtcggtg tcgctcgccg acttcacgca ggcgatcgag cgcatcgtcg
     8401 ccggcctcga gaagaagaac cgcctgctca acccgaagga gcgccggatc gtcgctcatc
     8461 acgagctcgg ccacgccatg gtggcgatgg cgctgccggg aacggatccg gtgcacaagg
     8521 tgtcgatcat cccgcgcggc gtgggcgcgc tgggctacac catccagcgc ccgatcgagg
     8581 accgcttcct catgacgcgc ggcgagctcg agaacaagat ggcggtgctg ctcggcggcc
     8641 gcgccgccga ggacgtggtg ttcggccacc tgtcgaccgg cgccgccgac gacctcgcga
     8701 aagtgaccga tatcgcgcgc agcatggtga tgcgctacgg catggtgaag agcctgggcc
     8761 acgtcgcgta cgacgaggag cgctcgccgt tcctggcggg gcagatcatc ccgaagagcc
     8821 gcgaatacag cgaggaaacc gcgcgcgaga tcgacgtcgc ggtgcgcgac atcgtcaagg
     8881 cctgctacga gaaggcgcgc ggcatccttt cccgggaaaa agcgctgctc gagagctggg
     8941 cgctgaagct gctcgagaaa gagacgctcg ccgaggccga gctcgccgag ctcagggctt
     9001 caatcagccc agcgtgagct agccgccggg acgcccccgc actaatgcgg cgcgctgctc
     9061 gggcggcgtc tgctcctcgt cgcgatagag gccgttgcgg aatacgaaag aggccgtcga
     9121 gtagaacgcg atggcttcgc gcaccagctg cggatcgtcg atctgctgga ggatgttctt
     9181 ctcggcgtcg acgcgaaacg cctcggtctt gcgccgggga tgcagcagtg ccatgcggtc
     9241 gcgcgccagg tagccgaggg tctggtagtt ggcgacgaac gcgcgatcgg actcgaccgg
     9301 cgatctgagg acgtcgcggc cgaagaactt gctgtagtag tcgaagtcaa gcaggccgag
     9361 caccgtcggc gggatatcga tctgcgacat caaccggtcg atgcggcgcg gcgcgacatg
     9421 cttgggcgcg tagacgaaca ccgggatcag gtacttgtcg accggaattc gcgtggtgcc
     9481 gcgcgcattg gcgccgtggt ccgcggtgat gacgaacagc gtatcgtcaa accacggctt
     9541 ggcgcgcgcc gcggcgagaa accggccgat ggcgtagtcg gtgtacttca cggcgccacc
     9601 gcgaccgctt cccgaaggga tgtcgatgcg cccggccgga tacgtatacg gccggtggtt
     9661 gctggtcgtc atcacgtgga cgaagaacgg acgctccggc ctggtcttgt gctcgcggtc
     9721 gatctcgtcg agcacctggt cgaacaggtg ctcgtcggcc acgccccaga ccgtttcatg
     9781 ctgcacgcga gccgaaggaa tggcgcgccg gtcgaccgag cggtagtcgt tcgcgtcgaa
     9841 gaatgcattc atgttgtcga aatagccgta gccaccgtac gcgaaaacca cggcgtaacc
     9901 cttatcctcg aacacgctgc cgagcgagaa cagcgtgtcg ttgttcggcc ggcgcacgat
     9961 cgactggccc ggcgtcggcg gcagggcgag cgacagcgcc tccatgccac gcacggtgcg
    10021 gttgccggtg gcgtacacgc gcgagaacca caggctctcg ctcgccaggc ggtccaaatg
    10081 cggcgtcagc ccgtccgggt tgccgtagcg gccgagaaat tccgcgccca ggctctccac
    10141 gctcaccagg acgacgttca gccgctgctc gggcccgggc gagtgcacgt gccgctcggt
    10201 gccctcgcct tgcgctccca gtgcgccgcg caccaggtcc atcgcctccg gcagcgggat
    10261 cgtcgcgtaa tggcggtcg
//
LOCUS       DATBSZ010000067        15776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_419144, whole genome shotgun sequence.
ACCESSION   DATBSZ010000067 DATBSZ010000000
VERSION     DATBSZ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15776
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419144"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..607
                     /gene="greA"
                     /locus_tag="VIG70_04365"
     CDS             137..607
                     /gene="greA"
                     /locus_tag="VIG70_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY8249817.1"
                     /translation="MKTPMTKRGAELLREELHRLKTIERPANSAAIGEARAHGDLSEN
                     AEYHAARERAGFIEGRISELEAKLGNAQVIDPKLVDADGRCVFGSTVDLEDESGEKAT
                     WQIVGEDEADIKKGRISVVSPMARALIGKESGETATVQTPGGVKKYEILDVRYE"
     gene            complement(617..2182)
                     /locus_tag="VIG70_04370"
     CDS             complement(617..2182)
                     /locus_tag="VIG70_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249818.1"
                     /translation="MLDVLDWVKKRIDDTAALQKARSARTDAPSIPRAAEPLTTLADL
                     TVRLKAAGDAGNDPRARSAALASIQDTAAVPVGALLTRYLGLGSASQAAREATWKSLV
                     GFQLRLAQAMCAAAGAELTAASAVHALAACRVLAKLHLMHYASVPGRLWHVAYALHAA
                     AEKAGVAGTPVHAQADPRTMTTVEQELLRLLMLHVSAPDMLTPQEIECADRAVERVGA
                     EFTLRQPGVADNPFCYEPAGEFGPRRAKGRQPAATTRHFGPGIGYESLERIAKQPGAA
                     KPEDFRPFGKDIAPRVQLSTVQHLLIFWRVDCPYAPPAHEPASGTMHVVHGYDRIWQE
                     ISRAPGGKHELSLAATSPGVAQLAETWQLRGVGGGELAAEVPPESRSWLKCGEVIGLS
                     VHDGAEHWMGMVRRIHAQPDGRLQADISLMSRAPKAHTLRKVLGKGEDRVFTEASAKQ
                     FGVSLVRALILADGAEPGRPANFMLPPGSWAEQCVFELQSDAGSRHLRGLQIVRRWDD
                     YVRATFEWVEVPG"
     gene            complement(2282..3361)
                     /gene="gcvT"
                     /locus_tag="VIG70_04375"
     CDS             complement(2282..3361)
                     /gene="gcvT"
                     /locus_tag="VIG70_04375"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY8249819.1"
                     /translation="MPNEKTVLHALHAELGARFVPFAGFDMPIQYREGLRAEHLHTRS
                     AAGLFDVSHMGQVRVTGPGLHESLERALPLDFDGWPEGVQRYSLLLNERGGIEDDLMV
                     TRLRDEVRIVVNASGREKDVALLRTLCPTLSFELLDRALIALQGPAAEDAFASLKPQA
                     RKLGFMQANAFDGWLVSRSGYTGEDGFEISVPTENAEPIVRQLLKDPRVKPIGLGARD
                     TLRLEAGLHLYGQDMDADTSVHEASLGWAIARSRRAGGASQGGFPGAERTLREAREGT
                     ARRLVGLAGEEPAPIRHGAALFASEEEAGVVTSGTVSPSTQRAIMLGYVGAKFLDQPL
                     SALVRDARRPVRIAKLPFVPKRYKK"
     gene            complement(3351..6161)
                     /gene="gcvP"
                     /locus_tag="VIG70_04380"
     CDS             complement(3351..6161)
                     /gene="gcvP"
                     /locus_tag="VIG70_04380"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEY8249820.1"
                     /translation="MTLDELLDKDGFAARHIGPPPAAQREMLAALGVSSLDALVREVV
                     PKDILLEQPLALGEAKTENEALAEISAHAGRNELWRNFIGMGYYGTHTPAVIQRNVLE
                     NPGWYTAYTPYQAEISQGRLEALLNFQQMVIDLTGLPVANASLLDEATAAAEAMAMIR
                     RSAKSKASAFFVDAAVHPQVLGVMRTRAKWLGIELVVGDEPQPEKVFGAYLQYPDTCG
                     RIRDFSAVIVKVHAAHGLVAMGTDLLALVLLRPPGALGADIAIGSAQRFGVPMGYGGP
                     HAAFMACRDEHKRSMPGRIIGVSVDAAGRPALRMALQTREQHIRRDKATSNICTAQAL
                     LANMAGFYAVYHGPRGLARIALRTNTMARLLAACVKPESSEFFDTLVFAVRSVEEVKG
                     RAGARRINLRYLDGARVGVSFDELTSLDDVADVAEALSGTRHNIQAIAKELAAEPGSI
                     PGALRRTDAVLSHPVFNRYHTETEMMRYLKRLENRDLSLVHAMIPLGSCTMKLNAAAE
                     LTPISWPRFAAMHPFAPPEQARGYAGMLRELEAMLAEITGFAAVSLQPNSGAQGEYAG
                     LLAIRSYLAAQGQGARDVCLIPASAHGTNPATAQMMGLRIVVVGCDENGNVDIADLER
                     KIAGHASRIAALMITYPSTHGVFEEAIVRICRLVHDAGGQVYMDGANMNALAGLAKPA
                     LIGADVCHINLHKTFCIPHGGGGPGMGPIAVASHLKAFLPDTTGPVSAAPYGSALILP
                     ISWMYIRMMGARGIREATEVAILNANYIAARLKRYFPVLYAGARGRVAHECILDLRKL
                     KESCGVSAEDVAKRLMDYGFHAPTLSFPVADTLMVEPTESESKDELDRFCDAMISIHG
                     ELEKIRSGAWDRAGNPVKNAPHTALELAGEWPHRYSREEAAFPKPWLRAAKYWPPVKR
                     IDNVAGDRNLVCTCPPIEAYAE"
     gene            complement(6161..6544)
                     /gene="gcvH"
                     /locus_tag="VIG70_04385"
     CDS             complement(6161..6544)
                     /gene="gcvH"
                     /locus_tag="VIG70_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY8249821.1"
                     /translation="MELPDKLKYTESHEWIADNGDGTVTVGITAVATEQLGDLVYVEL
                     PPVGSSVARGANCAIVESTKAASEVYAPVAGEVLAANTDLAKHPERVNQSPYQDGWLF
                     KLKVSDRAELDRLLVRDAYAKSAGV"
     gene            complement(6555..7931)
                     /locus_tag="VIG70_04390"
     CDS             complement(6555..7931)
                     /locus_tag="VIG70_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8249822.1"
                     /translation="MKRQKTITPVDGSVYVERDLASNSAIERALFMAEKAQKAWKQVP
                     VGERAAICRSMAEWCVSRANELATELSWQMGRPVAQSPGELHRGFRERALYMCSVAEK
                     TLEDLKVEPKPGFQRFIRREPLGVVFVVAPWNYPWLTSVNAVVPALLAGNSVILKMAA
                     QTPLVAERYAEAFKAAGLPQGVFQFLHLDHAQVAKVIADPRIAYVAFTGSVPGGQAVQ
                     QAAAARFIGTGLELGGKDPAYVRADADLPFAVENLVDGAYFNSGQSCCGIERIYVHQK
                     IHKGFVEAFVELARQYRLGNPLEKETNLGPLVRTQAADAVRAQIRQALKKGAKALLQL
                     KDRPRTPYLPPEVLINVDHGMDVMVEESFGPVVGIQAVKDDDEAVKLMNDSRYGLTAS
                     VWTPDVDAALRIGERVETGTWFMNRCDYLDPALAWTGLKDSGRGCSLSRLGLETFTRP
                     KSFHLRLT"
     gene            complement(7928..8650)
                     /gene="soxA"
                     /locus_tag="VIG70_04395"
     CDS             complement(7928..8650)
                     /gene="soxA"
                     /locus_tag="VIG70_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HEY8249823.1"
                     /translation="MKFCTVILSALVAAVSHAGEIPSAERRSGYEFMSRDTRAMQDDE
                     VTGPAVLWLLDGEALWGKLGCTGCHGDAKAGMKGVAARYPALVDGKLVNLEQQVRHKA
                     PALAYESRELLALTAYVARQSRGMPIQVNEKTKPLMDRGKDFFHRRQGQLNLACAQCH
                     DDNWGKKLAGNTIPQAHPTGYPIYRLEWQTVGSLARRFRNCLTGMRAELYAPGAPEFV
                     ELELYLMWRARGMKLESPAVRP"
     gene            complement(8647..8967)
                     /gene="soxZ"
                     /locus_tag="VIG70_04400"
     CDS             complement(8647..8967)
                     /gene="soxZ"
                     /locus_tag="VIG70_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEY8249824.1"
                     /translation="MARVVVNVPAEAKRGEVIEIRTLAGHPMETGFRRTQLGELIPRD
                     IIRSLVCTYNGVEVFRADFHPAMAANPLLTFTTVATESGTLEFRWTGDNGFSATQTAA
                     IKVL"
     gene            complement(8967..9431)
                     /locus_tag="VIG70_04405"
     CDS             complement(8967..9431)
                     /locus_tag="VIG70_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxY-related AACIE arm protein"
                     /protein_id="HEY8249825.1"
                     /translation="MRITRRAFLLLGAAAGTAHAQLPPNIAELRRQALDEAIRKVVGN
                     AQVRTGKVKLDLPPLIDNGNTVPLSVEVDSPMTAADHVKAIHVFTEKNPQPYVLSAHL
                     GPRSGRARITTRARIADTGVVTAIAQMSDGSFWSDSVKVIVTLSACLEEGLI"
     gene            complement(9436..9840)
                     /gene="soxX"
                     /locus_tag="VIG70_04410"
     CDS             complement(9436..9840)
                     /gene="soxX"
                     /locus_tag="VIG70_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEY8249826.1"
                     /translation="MRVLAAALAFLTSTAAADDAIPSSLTGTAGDASRGRAIVLDRHV
                     GLCLLCHSGPFPEEQFQGNLAPSLAGVGARLSAGQIRLRIVDSSRVNPDTIMPAYFRT
                     ENQARVAPAYRGKTVLNAQQIEDIVAFLVTLK"
     gene            complement(9852..12023)
                     /locus_tag="VIG70_04415"
     CDS             complement(9852..12023)
                     /locus_tag="VIG70_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY8249827.1"
                     /translation="MKMNRRSFIVSSAAVTGGLAVGFNPFQTNIVRAQDGSPELTAWV
                     VIQPNDTVIIRMARSEMGQGTITGLAQLVAEELECDWNKVTYEYPTPGTSLKRKRVWG
                     DFSTGGSRGIRTSHDYVRLGGATARVMLIQAAANQWKVPASECVAANSVITHKPSGRK
                     VTYGKVAEAAARLEPPKDVKLKDPKEWKLIGKPVKRLDTVDKVNGKQVYGFDLKLPGM
                     LNAAIKDCPVFGGKVKSFDAGRVTSMKGVKKVVPVGDSAVAVVAETWWQAKTALDALP
                     IQWDEGPNAKVSQEGILATLKEGLGASQAFVGNQNGDVKAGLGKATKTVEATYFYPFL
                     NHAPMEPMNATARYTPDRCEVWVPTQDGEASFAALLAASGLPADKCEVYKINLGGGFG
                     RRGAFQDYVTQAVIIAKQMPGVPVKLLWTREEDMLHGHYHPIMMAKLTGGLDASGNLV
                     GLHVRLSGQSILAAVRPHIVEQQKGRDPLTFQGLADTGEHSFGYSIPNLLIDHAMRNT
                     HVPPGFWRGVNINQNAVFMECFMDELAQAAGQDELEFRRKNMQGLPRNLGVLNAVAER
                     IGWSKPAKPGIYRGLAQMKAFNSFVAAACEISVQDGNKVKVHRIVAATDPGYAVNPAQ
                     IERQVSGSFVYGLSALFMQENTVKDGRIVEDNFHTFPSMRISQMPKVETIIIANGGGI
                     WGGIGEPTICVAAPAVMNAFFKATGKRLRDVPLKNQGIELV"
     gene            complement(12033..12491)
                     /locus_tag="VIG70_04420"
     CDS             complement(12033..12491)
                     /locus_tag="VIG70_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8249828.1"
                     /translation="MVRLTINGKAHNVDVDPNTPLLWVIREQVGLTGTKYGCGIAQCG
                     ACTVHVNGAPMRSCMLPVSTVVGQKITTIEGLASGKTLHRVQQAWIAHDVPQCGYCQS
                     GMIMAAAALLASKPKPTDADIDAAITNICRCGTFQEVRTAIHAAAKATKA"
     gene            12765..14006
                     /locus_tag="VIG70_04425"
     CDS             12765..14006
                     /locus_tag="VIG70_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8249829.1"
                     /translation="MEMIERGLSEEQRMMRASCRKFVDGVVTPFIRSHWQREWLMDPD
                     ARLPREILEQADRIGIRTLGVPEGFGGVQLDAATEVRTFALIAEEIARGDSGLADKLV
                     QNWKVSVLLRNFAPRRLQEKWFSRLVAEPGFLLAHCLTEPRGASDRWLPYNVPEAAMH
                     TRATESRDFWIINGRKQFISNGYDAGLFVVYANTNPKVGMLQGTSSFLVPRDTPGLSI
                     TKCNETIGCRFMNNGELVFEDMRVPKDHLLVQGDALGKAGIYFRPGKIIQAAKNLGVG
                     MAAYERTAEYVQSYVQGGRILIKHQAVALRLADMATRLSAVRSLIKEAALAVDENAGD
                     ADALCNMAKVFASEEIMKVVQHAMELHGGNGAMLDFGIEKLFRDGSIFLHMDATADIS
                     RLKIVKAMFPRTAGAYAGPEN"
     gene            complement(14003..14554)
                     /locus_tag="VIG70_04430"
     CDS             complement(14003..14554)
                     /locus_tag="VIG70_04430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY8249830.1"
                     /translation="MKILGISGSLRKGSYNTALLRAAAGLMPDGAQLEVGSIAGIPLY
                     DGDLEASQGIPPAVQTLKKSITEAQGLLLVTPEYNNSIPGVFKNAIDWLSRPSSDIAK
                     VFGGRPVGVIGASPGGFGTILSQNAWLPVLRTLGTRPFFGGRLQVSRAGNVFNPAGEL
                     ADEAIGGQLAKYLAGFVSFIKNP"
     gene            complement(14551..15312)
                     /locus_tag="VIG70_04435"
     CDS             complement(14551..15312)
                     /locus_tag="VIG70_04435"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY8249831.1"
                     /translation="MRLKDKVALITGAGSGIGRQTALLFSKEGAAVVCVDVNENAARE
                     TASSIRNSIAVKADVSNAADCRQMVMAAERQFGKLHVLFNNAGIMHAKDDDAMSTSEE
                     VWDLTMSINAKGVFLGCKYGIPAIQRAGGGSVINTASFVAVLGAATPQIAYTASKGAV
                     LSMTRELAVIHARQKIRVNALCPGPLKTELLMSFLNTEAKKQRRLVHIPMGRFGEAEE
                     IAQAALYLASDESSYVTGTEFLVDGGITAAYVTPE"
     gene            complement(15309..>15776)
                     /locus_tag="VIG70_04440"
     CDS             complement(15309..>15776)
                     /locus_tag="VIG70_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY8249832.1"
                     /translation="VPDVMVFYAPTVNSYKRYVDASWAPTRLAWSNDNRTAGFRIVGS
                     GPSTRIECRIPGADCNPYLALAASLASGLDGIASKIEPPEVFVGDIYQAKDLPRVPYT
                     LSQATDLFEKSAFAKKAFGKDVHEHYTHFYRTEAAAYDKAVTDWERRRYFERI"
BASE COUNT         2465 a         5210 c         5380 g         2721 t
ORIGIN      
        1 gcgtcaccta ctacacaacg gtggccggtg ccaaggcggc ggtcgccggc atgaagcatc
       61 tggcgcagct cgagccatac cgcctgcagg acctgcacgc caggcttcgc caggcgtccc
      121 cggaggccgt tgccccatga agacccccat gacaaagcgc ggcgccgagc tgctgcgcga
      181 agagctgcac cgcctgaaga ccatcgagcg gcccgccaac tcggcggcga tcggcgaagc
      241 gcgcgcgcac ggcgacctgt cggagaacgc cgagtaccac gcggcgcgcg agcgtgccgg
      301 cttcatcgag ggccgcatct cggagctcga ggccaagctt ggcaacgctc aggtgatcga
      361 tcccaagctg gtcgacgccg acggacgctg cgtgttcggc tcgaccgtcg atctcgagga
      421 cgagtcgggc gagaaggcga cctggcagat cgtcggcgag gatgaggccg acatcaagaa
      481 gggacgcatc tcggtcgtct cgccgatggc gcgcgccctg atcggcaagg agtccggcga
      541 gaccgccacc gtgcagaccc cgggcggcgt gaagaaatac gagatcctcg acgtccggta
      601 cgagtagcgg cgcgcgtcag ccgggcactt ccacccactc gaaggtcgcg cgcacgtagt
      661 cgtcccagcg gcgcacgatc tgcaggccgc gcaggtggcg cgatccggcg tcgctctgca
      721 gctcgaatac gcattgctcc gcccagctcc ccggcggcag catgaaattc gccgggcgcc
      781 cgggctcggc gccgtcggcg aggatcaggg cgcgcaccag gctcacgccg aactgcttgg
      841 ccgaggcctc ggtgaacacg cgatcctcgc ctttcccgag cactttgcgc agcgtgtgcg
      901 ccttgggcgc gcggctcatc agggagatgt ccgcctgcag gcgcccatcg ggctgggcat
      961 ggatgcggcg gaccatcccc atccagtgct cggcgccgtc gtgcaccgac aggccgatca
     1021 cctcgccgca cttcagccag gagcggctct ccggcggtac ttccgcggca agctcgccgc
     1081 cgccgacgcc ccgcagctgc caggtttccg cgagctgtgc cacgccgggc gacgtggcgg
     1141 cgagcgacag ttcatgcttg ccgcccgggg ccctggagat ctcttgccag atccggtcgt
     1201 agccatggac gacgtgcatg gtccccgacg ccggctcgtg cgcgggcggt gcgtaggggc
     1261 aatcgacgcg ccagaagatg agcaggtgct gcacggtgct cagctgcact ctcggcgcga
     1321 tgtccttgcc gaagggcctg aagtcttcgg gcttcgccgc gcccggctgc ttggcgatgc
     1381 gctcgagcga ctcgtacccg atcccggggc cgaagtgacg cgtagtcgca gccggctgcc
     1441 gccccttggc acgccgtggg ccgaactcgc ctgcgggctc gtagcagaac gggttgtcgg
     1501 cgacgccggg ctggcgcagc gtgaactcgg cgcccacgcg ctcgacggcg cggtcggcgc
     1561 actcgatctc ctgcggcgtc agcatgtcgg gtgcgctcac gtgcagcatg agcaggcgca
     1621 ggagctcctg ctccacggtg gtcatggtcc gggggtcggc ctgcgcgtgc accggcgtgc
     1681 ccgcgacgcc ggccttctcg gcggccgcat gcagtgcgta cgccacgtgc cacagccgtc
     1741 ccggcacgct cgcgtaatgc atgagatgca gcttggcgag gacgcggcag gctgccagcg
     1801 cgtgcaccgc gcttgcggcg gtgagctcgg cgcccgccgc cgcgcacatt gcctgcgcca
     1861 ggcgcaactg gaaaccgacc agcgacttcc aggtggcttc gcgcgcagcc tgcgaggcgc
     1921 tgccgaggcc gagataccgc gtgagcagcg cgcctaccgg gacagcggcc gtgtcctgga
     1981 tcgacgcgag agcggcgctg cgcgccctgg gatcgttacc ggcgtcgccc gccgccttca
     2041 gccgcacggt cagatccgcg agcgtggtga gcggctcggc ggcgcgagga atcgaaggcg
     2101 catcggttct cgccgacctg gccttctgca gcgcggccgt gtcgtctatg cgcttcttga
     2161 cccaatccag aacgtccagc atcgacgccc tcccgggtgc gctccccaga agattgtagg
     2221 cctcggggga ggcggcatcc gtgacaatag tcaccggccg cgccccattc ttctcttcaa
     2281 cctacttctt gtagcgcttc ggcacgaagg ggagcttggc gatgcgcacc gggcggcgcg
     2341 catcgcgcac cagggccgaa aggggctggt cgagaaattt cgcccctacg tagcccagca
     2401 tgatggcgcg ctgggtcgac ggcgatacgg tgccgctcgt cacgacaccg gcttcctctt
     2461 cggaagcaaa gagcgcagcg ccgtggcgga tcggcgccgg ctcttcgccg gccaggccga
     2521 ccagccggcg cgcggtgcct tcgcgcgctt cacgcagggt gcgctcggcg ccgggaaagc
     2581 cgccctggct ggcgccgccg gcgcggcgcg agcgcgcgat cgcccagccc aggctggcct
     2641 cgtggacgct cgtgtccgca tccatgtcct ggccgtagag gtgcagcccg gcctccaggc
     2701 gcaaggtgtc acgggcgccg agcccgatgg gtttcactct ggggtctttc aggagctgcc
     2761 ggacgattgg ctcggcgttt tccgttggca cggaaatctc gaagccgtct tcccccgtgt
     2821 agcccgagcg ggagacgagc cagccgtcga aagcgttggc ttgcatgaac ccgagcttgc
     2881 gagcttgcgg cttgagtgac gcaaaggcgt cttcagctgc agggccctgg agagcgatca
     2941 gcgcgcggtc gaggagctcg aacgacaggg tggggcagag cgtgcgcagc agcgcgacgt
     3001 ccttctcgcg gccgctcgcg ttgaccacga tgcgcacttc gtcgcgcagg cgcgtgacca
     3061 tgagatcgtc ctcgatcccg ccgcgctcgt tgaggagcag cgagtagcgc tgcacgccct
     3121 cgggccagcc gtcgaaatcg agcggcagcg cgcgctcgag cgattcgtgc agcccggggc
     3181 cggtgacgcg cacctgcccc atgtgggaga cgtcgaaaag tcccgccgcg ctgcgcgtgt
     3241 gcagatgctc cgcgcgcagg ccctcgcggt actggatcgg catgtcgaac ccggcgaagg
     3301 gcacgaagcg cgcgccgagc tccgcatgca gtgcatgcag tacggtcttt tcattcggca
     3361 tacgcttcga tcggcgggca cgtacagaca aggttgcggt cgcccgcgac gttgtcgatg
     3421 cgcttcaccg gcggccagta cttcgcggcg cgcagccacg gcttggggaa cgcggcttcc
     3481 tcgcggctgt accgatgcgg ccattcgccc gcgagctcga gcgcggtgtg cggcgcgttc
     3541 ttgaccgggt tgccggcgcg gtcccacgcg ccggagcgga tcttctccag ctcgccgtgg
     3601 atcgagatca tcgcgtcgca gaagcggtcg agctcgtcct tcgattcgct ctcggtcggc
     3661 tcgaccatca gcgtgtcggc caccggaaac gagagcgtcg gggcgtggaa gccgtagtcc
     3721 atcagccgct tcgcgacgtc ctcggcgcta acgccgcagc tttccttcag cttgcgcaga
     3781 tccaggatgc actcgtgcgc gacgcgcccg cgcgcgccgg catagagcac cgggaagtag
     3841 cgcttgagcc gtgcggcgat gtagttcgcg ttcaggatcg cgacctcggt cgcctcgcgt
     3901 attccccgcg cgcccatcat gcggatgtac atccacgaga tcggcaggat gagggcgctg
     3961 ccgtagggag cggccgacac cggcccggtc gtgtcgggca agaaagcttt caggtgcgag
     4021 gcaacggcaa tcggccccat cccgggccct ccgccgccgt gcgggatgca gaaggtcttg
     4081 tgcaggttga tgtggcagac gtcggcgccg atcagggcgg gcttggcgag cccggcgagc
     4141 gcgttcatgt tggcgccgtc catgtacacc tggccgccgg catcgtgcac cagcctgcag
     4201 atgcgcacga tcgcctcctc gaacacgccg tgcgtcgagg ggtacgtgat catcagggcg
     4261 gcgatgcggc tcgcgtgccc ggcgatcttg cgctcgaggt cggcgatgtc gacgttgccg
     4321 ttctcgtcgc agccgacgac gacgatgcgc agccccatca tctgggcggt ggcggggttg
     4381 gtgccatgcg ccgaggcggg gatgaggcag acgtcgcgcg cgccttgtcc ctgcgcggcg
     4441 agatagctgc ggatggcgag gagtcccgcg tactcgcctt gcgcgccgga attgggttgc
     4501 agcgaaaccg cggcgaagcc ggtgatctcg gcgagcatcg cttccagctc gcgcagcatg
     4561 ccggcgtagc cccgcgcctg ctcgggcggc gcgaacgggt gcatcgccgc aaagcgcggc
     4621 cacgagatcg gcgtgagctc ggccgcggcg ttgagcttca tcgtgcacga gccgagcgga
     4681 atcatcgcgt gcaccagcga caggtcgcgg ttctccagcc gcttgaggta gcgcatcatc
     4741 tcggtctcgg tgtggtagcg gttgaagacc ggatgcgaga gcacggcgtc ggtgcggcgc
     4801 aaagcccccg ggatggaacc gggttctgca gccaattcct tcgcgatggc ttgaatattg
     4861 tgtcttgtcc ctgaaagcgc ctccgcgacg tccgccacgt cgtccaacga ggtgagctcg
     4921 tcgaagctca ctcccacgcg agcgccgtcc aggtagcgca ggttgattcg ccttgcgccc
     4981 gcgcggccct ttacttcctc gacggagcgc acggcgaaaa ccagggtgtc gaaaaattcg
     5041 ctgctctcgg gcttgacgca cgcggcgagc agacgagcca tggtgttcgt gcgcagcgcg
     5101 atgcgcgcca gcccccgcgg tccgtggtac acggcgtaga acccggccat gttggcgagc
     5161 agggcctggg cggtgcagat gttgctcgtg gccttgtcgc ggcggatgtg ctgctcgcgc
     5221 gtctgcagcg ccatgcgcag cgccggcctg cccgcggcat cgaccgagac gccgatgatc
     5281 ctgccgggca tcgagcgctt gtgctcgtcg cggcacgcca tgaacgcggc gtgcgggccg
     5341 ccgtagccca tcggcacgcc gaagcgctgc gccgagccga tcgcgatgtc cgcgccgagc
     5401 gcgcccggcg ggcgcagcag cacgagcgca agcaggtcgg tgcccatggc gacgaggccg
     5461 tgggcggcat ggactttcac gatgactgcg gagaaatcgc ggatgcggcc gcaggtatcg
     5521 ggatattgca ggtacgcgcc gaagactttc tccggctgag gttcatcgcc gacgacgagc
     5581 tcgatgccga gccactttgc gcgcgtgcgc atgaccccga ggacctgcgg atgcaccgcc
     5641 gcgtcgacga agaacgcgct cgccttgctc ttcgcgctgc gccggatcat cgccattgcc
     5701 tccgccgcgg cggtcgcttc gtcgagcagc gaagcgttcg ctaccggcag cccggtgagg
     5761 tcgatcacca tctgctggaa attgaggagc gcctcgagcc ggccttgcga gatctccgcc
     5821 tggtagggcg tgtacgcggt gtaccagccg gggttctcga gcacgttgcg ctggatcacg
     5881 gccggcgtgt gcgtgccgta gtaccccatg ccgatgaagt tgcgccagag ctcgtttcgc
     5941 cccgcgtgcg cgctgatctc cgcgagcgcc tcgttctcgg tcttcgcttc tccgagcgcg
     6001 agcggctgct cgagcaggat gtccttcggc acgacctcgc gcaccagcgc gtcgagcgag
     6061 gacacgccga gggccgcgag catctcgcgc tgcgccgccg ggggcggacc gatgtggcgg
     6121 gcggcgaaac cgtccttgtc gagcagctcg tccagcgtca tcagacgccc gcggacttcg
     6181 cgtaggcgtc gcgtacgagc agccggtcga gctccgcgcg atcgctgacc ttgagcttga
     6241 acagccaccc gtcctgatag ggcgactggt tcacccgctc gggatgcttg gcgaggtctg
     6301 tattcgcggc gagcacctcg ccggcgaccg gcgcatagac ttccgaggcg gccttggtgg
     6361 actccacgat cgcgcagttc gcgccgcgcg cgacgctcga gcccaccggg ggcagctcga
     6421 cgtagacgag gtcgccgagc tgctcggtcg ccaccgcggt gatgccgacg gtgacggtgc
     6481 cgtcgccgtt gtcggcgatc cattcgtgcg attcggtgta cttgagcttg tcgggcagct
     6541 ccaaggcgtc ctcctcaggt tagccgcaag tgaaacgact tcgggcgcgt gaacgtctcc
     6601 agcccgagcc tcgaaagcga gcagccgcgc ccggagtcct tcaacccggt ccaggcgagc
     6661 gccggatcga ggtagtcgca gcggttcatg aaccaggtgc cggtctcgac gcgctcgccg
     6721 atgcgcagcg ccgcgtcgac gtccggcgtc cagaccgagg cggtgaggcc gtagcggctg
     6781 tcgttcatca gctttaccgc ctcgtcgtcg tctttcaccg cctggatacc gaccaccggc
     6841 ccgaagctct cctcgaccat cacgtccatg ccgtggtcga cgttgatcag cacctcgggg
     6901 ggcagatagg gcgtgcgcgg ccggtctttg agctgcagca gggctttcgc ccctttcttc
     6961 aacgcctggc ggatctgggc gcgcaccgcg tccgccgcct gggtgcgcac caacggcccg
     7021 aggttggtct ccttttccag cggattgccg agccggtact gacgcgcgag ctcgacgaag
     7081 gcctccacga atcctttgtg gattttctga tgcacgtaga tgcgctcgat cccgcagcag
     7141 gattgcccgg aattgaagta cgcgccgtcg acgaggttct ccaccgcgaa cggcaggtcc
     7201 gcgtcggcgc gcacatacgc ggggtccttg ccgccgagct cgaggccggt gccgatgaag
     7261 cgcgcggcgg cggcttgctg caccgcctgc ccgccgggca ccgagccggt gaacgccacg
     7321 taggcgatgc gcggatccgc gatcaccttg gcgacctgcg cgtggtcgag gtgcaggaac
     7381 tggaacacgc cctgcggcag gccggcggcc ttaaacgcct cggcatagcg ctcggcgacg
     7441 agcggcgtct gcgccgccat cttcaggatc acgctgttgc ccgcgagcag cgccggcacc
     7501 accgcgttca ccgaggtgag ccagggatag ttccacggcg ccaccacgaa caccacgccg
     7561 agcggctcgc gccggatgaa gcgctgaaag cccggctttg gctccacctt gagatcctcg
     7621 agggtctttt ctgcgacgct gcacatgtaa agcgcgcgct cgcggaaccc gcgatgcagc
     7681 tcgcccgggc tctgcgcgac cggccgcccc atctgccagc ttagctcggt ggcgagctcg
     7741 ttggcccggg acacgcacca ttcggccatg ctgcggcaga tcgcggcgcg ctcgccgacc
     7801 ggcacctgct tccaggcctt ctgcgccttc tccgccatga agagggcgcg ctcgatcgca
     7861 gagttcgacg ccaggtcccg ctctacgtaa accgagccgt cgaccggcgt gatggttttt
     7921 tgccgcttca tggacgcacc gccggggatt ctagtttcat gccgcgcgcg cgccacatga
     7981 ggtaaagctc gagctcgacg aattccggcg cgccgggcgc gtagagctcg gcgcgcatgc
     8041 cggtgaggca gttgcggaat cgccgcgcga gcgagcccac ggtctgccat tccagccggt
     8101 aaattggata gccggtcgga tgcgcctgcg ggatggtgtt gccggcgagc ttcttgcccc
     8161 agttgtcgtc gtggcactgg gcgcacgcga gattgagctg gccctggcga cgatggaaga
     8221 aatcctttcc tcggtccatt agaggcttcg tcttttcatt tacttgaata ggcatgccgc
     8281 gagactgccg cgccacgtaa gccgtcagcg cgagcagctc gcggctctcg taggcgagcg
     8341 cgggcgcttt gtgacgaacc tgctgctcca ggttgacgag cttcccgtcc accagcgccg
     8401 ggtagcgcgc ggccacgcct ttcatgccgg ccttcgcgtc gccgtggcag ccggtgcagc
     8461 cgagcttgcc ccacagcgcc tcgccgtcga gcagccacag caccgccggc ccggtgacct
     8521 cgtcgtcctg catggcgcgc gtgtcgcggc tcatgaattc gtagccgctg cggcgctcgg
     8581 ccgaggggat ttcacccgcg tgggaaacgg cggcaaccag cgccgaaagg atgacggtgc
     8641 aaaacttcaa agtaccttga tcgccgccgt ctgggtcgcg gagaagccgt tgtcgccggt
     8701 ccagcggaat tccagggtgc cgctctccgt tgcgacggtg gtgaaggtga gaagcggatt
     8761 ggccgccatc gcgggatgga aatcggcgcg gaacacctcg acgccattgt aggtgcacac
     8821 caggctgcgg atgatgtcgc gcgggatcag ctcgccgagc tgggtgcggc ggaagccggt
     8881 ctccatcggg tggccggcga gggtgcggat ctcgatcacc tcgccgcgct tcgcctctgc
     8941 cgggacgttg accacgacgc gcgccatcag atcagtcctt cctcgaggca tgccgaaagc
     9001 gtaacgatca ccttcacgct atcggaccag aacgagccat cgctcatctg cgcaatggcg
     9061 gtgacgacgc cggtatcggc gatgcgcgcg cgcgtcgtga tgcgcgcgcg gccggagcgc
     9121 gggccaaggt gcgcgctgag cacgtagggc tgcgggttct tctcggtaaa gacgtgaatc
     9181 gctttcacat ggtcggccgc ggtcatcggg ctgtcgacct cgaccgagag cggcaccgtg
     9241 ttgccgttgt cgatgagcgg cggcaggtcg agcttgacct tgccggtgcg cacctgggcg
     9301 ttgccgacga ccttgcggat cgcttcgtcg agcgcctgcc tgcgcagctc ggcgatgttg
     9361 gggggaagct gcgcgtgggc ggtccccgcg gcagcaccca acagcaagaa ggcgcggcga
     9421 gtgatcctca ttttcctatt tcagcgtgac gaggaaggcg acgatgtctt cgatctgctg
     9481 cgcattgagc acggtcttgc cgcgataggc gggcgccacg cgcgcctggt tttcggtcct
     9541 gaagtacgcc ggcatgatgg tgtccggatt gacgcggctc gagtcgacga ttctgagccg
     9601 gatctggccc gccgacaatc tcgcccccac gccggcgagg ctcggcgcga gattgccctg
     9661 gaactgctcc tcggggaacg ggccgctgtg gcacagcagg cacaggccga cgtgcctgtc
     9721 caacacaatg gcgcggccgc gcgaggcgtc tcccgccgtc ccggtgaggg acgacgggat
     9781 cgcatcatct gcggcagccg tgctggtgag gaaggcgagg gcggcggcca acaccctcac
     9841 acgaacctca tctacaccaa ttcgatgccc tggttcttga gcgggacgtc gcgcaggcgc
     9901 ttgcccgtgg ccttgaagaa ggcgttcatc acggccggcg cggcgacgca gatcgtgggc
     9961 tcgccgatgc cgccccagat cccgccgccg ttggcgatga tgatcgtctc caccttcggc
    10021 atctgcgaga tgcgcatcga cgggaaggtg tggaagttgt cctccacgat gcgtccgtcc
    10081 ttgaccgtgt tctcctgcat gaacagcgcc gagaggccgt agacgaacga gcccgagacc
    10141 tggcgctcga tctgcgccgg gttcacggcg tagcccgggt cggtcgccgc gacgatgcgg
    10201 tgcaccttca ccttgtttcc gtcctggacc gagatctcgc acgccgccgc gacgaagctg
    10261 ttgaaggcct tcatctgcgc caggccgcgg tagatccccg gtttcgcggg cttgctccag
    10321 ccgatgcgct cggccaccgc gttcagcacg ccgaggttgc gcggcaaacc ctgcatgttc
    10381 ttgcgccgga attcgagctc gtcctggccc gcggcctgcg ccagctcgtc catgaagcat
    10441 tccatgaaca ccgcgttctg gttgatgttg acgccgcgcc agaagcccgg cggcacgtgc
    10501 gtgttgcgca tcgcgtggtc gatgagcagg ttcgggatgc tgtagccgaa cgagtgctcg
    10561 ccggtgtcgg cgaggccctg gaaggtgagc ggatcgcgcc ccttctgctg ctcgacgatg
    10621 tgcggtcgca ccgccgcgag gatcgactgg cccgacaggc gcacgtgcag accgaccagg
    10681 ttgccgctcg cgtcgagccc gccggtcagc ttcgccatca tgatcggatg gtagtgcccg
    10741 tgcagcatgt cctcttcacg cgtccacagc agcttcaccg gcacgccggg catctgcttc
    10801 gcaataatga ccgcttgcgt gacgtagtcc tggaacgcgc cgcgccggcc gaagccgccg
    10861 ccgaggttga tcttgtagac ctcgcacttg tccgccggca ggccggaggc cgcgagcagc
    10921 gcggcgaacg aggcttcgcc gtcctgcgtc ggcacccaga cctcgcagcg gtcgggcgtg
    10981 tagcgcgcgg tggcattcat cggctccatc ggcgcgtggt tcaagaaggg gtagaagtac
    11041 gtcgcttcga cggtcttcgt agctttgccg aggcccgcct tgacgtcgcc gttctggttg
    11101 ccgacgaaag cctgcgacgc gccgaggcct tccttcagcg tggcgaggat gccttcctgc
    11161 gacaccttgg cgttcgggcc ttcgtcccac tggatcggca gcgcatcgag cgcggtcttc
    11221 gcctgccacc aggtttcggc gaccacggcg acggcggagt cgccgaccgg cacgaccttt
    11281 ttcacgcctt tcatgctggt gacgcggccg gcgtcgaagc tcttcacctt gccgccgaac
    11341 accgggcagt ccttgatcgc ggcgttcagc atgcccggca gcttgaggtc gaagccgtac
    11401 acctgcttgc cgttcacctt gtccacggtg tcgagccgct tcaccggctt gccgatgagc
    11461 ttccactcct tcgggtcctt gagcttgacg tccttgggcg gctcgaggcg cgcggcggcc
    11521 tcggcgacct tgccgtacgt caccttgcga cccgacggct tatgggtgat gacgctgttc
    11581 gccgccacgc attcggacgc cggcaccttc cactggtttg cggcggcctg gatcagcatc
    11641 acgcgcgcgg tcgcgccgcc caggcgcacg tagtcgtgcg aggtgcggat gccgcggctg
    11701 ccgccggtgg agaagtcacc ccagacgcgc ttgcgcttga ggctggtgcc cggcgtcggg
    11761 tattcgtacg ttaccttgtt ccagtcgcac tcgagctcct cggcgaccag ctgcgccagg
    11821 ccggtaatgg ttccctggcc catctcggag cgcgccatgc ggatgattac ggtgtcgtta
    11881 ggctggatca cgacccaggc ggtgagctcg ggcgacccgt cttgagcgcg aacgatgttg
    11941 gtttgaaacg ggttgaaacc gacagcaaga cccccagtga cggcggcgga gctgacgatg
    12001 aacgagcggc gattcatttt catggctccc ccttatgcct tcgtcgcttt cgcggcggcg
    12061 tggatcgccg tgcgcacttc ctgaaacgtg ccgcagcggc agatgttggt gatcgcggcg
    12121 tcgatgtcgg cgtcggtcgg cttcggcttg gatgcgagca gcgccgcggc cgccatgatc
    12181 atgccggact ggcagtagcc gcactgcggc acgtcgtgcg caatccaggc ctgctgcact
    12241 ctgtgcagcg tcttgccgga ggcaaggcct tcgatggtgg tgatcttctg gccgacgacg
    12301 gtgctcaccg gcagcatgca ggagcgcatc ggcgcgccat tgacgtgcac ggtgcaggcg
    12361 ccgcactggg cgatgccgca accgtacttg gtgccggtca acccgacctg ctcgcggatc
    12421 acccagagca gcggcgtatt cggatcgacg tctacgttat gagccttgcc attgatggtt
    12481 aagcgaacca ttgcctgcct cctcccggtg ggtaactggt cgcgaatcga ggtcgctcag
    12541 ctactctagt ccggtagctt cggcggcggc aacagctttt gcgagattgc ggcgtcgcag
    12601 cagccgggcg cctaggcgac cagcacgctc accgtgacga ggacgaggcc gatggaataa
    12661 acccagtaat caagcgtgct catgccggta tcgtggcgcc ggcggcttag caatcgattt
    12721 caggggcttg tgtacgtttt acaactgaaa caagggcgaa cagcatggag atgatcgagc
    12781 ggggactgtc cgaagagcag cgcatgatgc gcgcgagctg ccggaaattc gtggacggcg
    12841 tggttacgcc gtttatccgt agccactggc agcgcgaatg gctgatggat ccggacgcgc
    12901 ggctgccgcg cgaaatcctg gagcaggccg accgcatcgg catccgcacc ctcggcgttc
    12961 ccgagggatt cggcggcgtg cagctcgacg cggcgaccga ggtgcgcacc tttgccctga
    13021 tcgcggagga aatcgcccgc ggcgactcgg gcctcgccga caagctggtg cagaactgga
    13081 aagtgtcggt gctgctgcgc aacttcgcgc cgcgccgcct gcaggagaaa tggttttcaa
    13141 gattggtcgc cgaaccgggt ttcctgctgg cccactgcct cacggagccc cgcggcgcgt
    13201 ccgatcgctg gctgccgtac aacgtgccgg aagcggccat gcacacgcgg gcaaccgaat
    13261 ccagggattt ctggattatt aatggccgca agcagttcat cagcaacggc tatgacgcgg
    13321 gcctgtttgt ggtctacgcc aacacgaatc ccaaggtggg gatgctgcag ggcacctcca
    13381 gcttcctcgt gccgcgagat acgcccgggc tgagcattac gaaatgcaat gaaaccatcg
    13441 gctgccgctt catgaacaac ggcgagctgg tattcgagga catgcgcgtg cccaaggacc
    13501 accttctcgt ccagggcgac gcgctcggca aggccgggat ctacttccgg cccggcaaga
    13561 tcatccaggc ggcgaagaac ctgggggtcg ggatggcggc ctacgagcgc accgccgagt
    13621 acgtgcagag ctacgtccag ggcggcagga tcctcatcaa gcaccaggcg gtggctttgc
    13681 gcctcgcaga catggcgacg cgactctctg cggtcagatc cttgattaaa gaagctgctc
    13741 tggcagtgga cgaaaacgca ggggatgccg acgccctctg caacatggcg aaggtgttcg
    13801 cttccgagga gatcatgaag gtggtgcagc acgcgatgga gctgcacggc ggcaacggcg
    13861 cgatgctcga cttcggcatc gagaagctgt tccgcgacgg ctcgatcttc ctgcacatgg
    13921 acgccaccgc cgacatctcg cgcttgaaga tcgtgaaggc gatgttcccg cgcaccgccg
    13981 gcgcctacgc cggccccgag aattagggat tcttgataaa agaaacgaac ccggcgaggt
    14041 acttggcgag ctggccgccg atcgcctcgt cggcaagctc cccggctgga ttgaacacat
    14101 tgccggcgcg cgagacctgc aggcggccgc cgaagaaggg ccgcgtgccc agcgtccgca
    14161 gcaccggcag ccaggcgttc tgcgagagga tggtgccgaa cccgccgggc gaggcgccga
    14221 tcacgccgac cggccggccg ccgaagacct tggcgatgtc ggaagagggg cgcgagagcc
    14281 agtcgattgc gttcttgaac acgcccggga tcgagttgtt gtattcgggg gtgaccagga
    14341 gcagcccttg cgcctcggta atgctctttt tcaaggtttg aactgcagga ggaatgccct
    14401 gcgatgcctc caggtcgccg tcgtagagcg ggattccggc gatcgagccg acctcgagct
    14461 gcgcgccgtc gggcatcagc ccggccgccg cgcgcaggag ggcggtgttg tacgagcctt
    14521 tgcgcaggct tccggagatc ccgagaatct tcattccgga gtcacgtaag cggcggtgat
    14581 cccgccgtcg acgaggaact cggtgccggt gacgtaggac gattcgtccg aggcgaggta
    14641 cagcgccgcc tgggcgatct cttcggcttc cccgaagcgc cccatcggga tgtggacgag
    14701 gcggcgctgc ttcttcgctt ctgtattcag gaagctcatc aggagctcgg ttttcagggg
    14761 gccgggacag agcgcattga cccggatctt ctgccgcgca tggatcaccg cgagctcgcg
    14821 ggtcatcgag agtactgcac ccttgctcgc ggtgtaggcg atctgcggcg tggccgcgcc
    14881 caagaccgcc acgaacgacg ccgtattgat caccgagccg ccgcccgcgc gctgaatcgc
    14941 cgggatgccg tacttgcagc cgaggaacac gcccttggcg ttgatgctca tcgtgaggtc
    15001 ccagacctcc tcgctggtcg acatcgcgtc gtcgtccttc gcgtgcatga tcccggcgtt
    15061 gttgaacagc acgtgcagtt ttccgaattg ccgctcggcg gccatgacca tctgccggca
    15121 atcggcggcg tttgagacat ccgccttgac ggcaatggaa ttcctaatgg aagaagcggt
    15181 ctcgcgagcc gcgttctcgt tcacatctac gcaaacgacc gccgcgccct ccttggagaa
    15241 gagaagcgcg gtttgccgcc cgatgccgct gccggcgccc gtgatgagcg ccactttgtc
    15301 tttcagtctc atatccgctc gaagtatctc ctgcgttccc agtcggtgac cgctttgtcg
    15361 taagccgcgg cttcggtgcg atagaagtgg gtgtagtgct cgtgcacgtc tttgccgaac
    15421 gctttcttcg cgaaagcgct tttttcgaac agatcggtgg cctggctgag cgtgtagggc
    15481 acccgcggca ggtccttggc ctggtagatg tcgccgacga agacttccgg cggctcgatc
    15541 ttgctggcga tgccgtcgag gccagaggcg agggaggcgg caagggcgag atacggattg
    15601 cagtcggcgc ccgggatgcg gcactcgata cgcgtgctcg gtcccgagcc cacgatgcgg
    15661 aatccggcgg tgcggttgtc gttgctccag gcaagccgcg tcggcgccca ggacgcgtcg
    15721 acgtagcgct tgtaggagtt caccgtcggc gcatagaaca ccatcacgtc gggaac
//
LOCUS       DATBSZ010000068         9001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_282845, whole genome shotgun sequence.
ACCESSION   DATBSZ010000068 DATBSZ010000000
VERSION     DATBSZ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282845"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1393
                     /locus_tag="VIG70_04445"
     CDS             104..1393
                     /locus_tag="VIG70_04445"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY8249833.1"
                     /translation="MATMLRRVAVLGGVRLPFARANGAYNELSNQHMLTAAMAALVER
                     FQLKNQKIGEVAAGAVIKHSRDWNLARECVLGSGFDPATPAYDLQRACGTSLSAVAQL
                     ANRIAQGEIDSGVAGGADSASDIPLSYSPGLQRAVLSAARNKSWTGKVASIGSLRPRD
                     LAPAAPGIAEPRTGLSMGQHCEEMAKEWAIGRREQDELALASHKNAAAAWRDGFFDDL
                     TVEFSGLKTDNNVRGDTSLEKLSSLRTAFDPSPAGTLTAGNSSPLTDGAACVLLGSEE
                     WARSHGLEVQCFITHSETAAVDFVGMNGAKEGMLMAPAYAVPRMIDRAGLKLQDFDIY
                     EIHEAFAAQVLCTLKAWESEEYCRSKLKRAGALGRVERTKLNPKGSSVALGHPFAATG
                     ARIVATLAKQLAQRGSGRGLISICAAGGMGVTAILER"
     gene            complement(1390..2367)
                     /locus_tag="VIG70_04450"
     CDS             complement(1390..2367)
                     /locus_tag="VIG70_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8249834.1"
                     /translation="MLSRYKKLLEQRPASWDPGFRSLWGFFLGATPRATQLVARIQDL
                     GAQRIADMDATGIARQLVFLSAPGVQVFDSATAVSLSTDLNDQLLQKIRQYPERYAAL
                     AAIAPQAPNAAAAELQRSVKKLGFKGAVINSHTQGEYLDDPKYWEIFEAAEALDVPLY
                     LHPNTPPPAMIDPFLPRGLDGAIYGFAVETGLHLLRIVVAGVFDRFPKLRMVVGHLGE
                     GLPFWLFRIDFMHRAMVAAKRYPGVQALKKKPSDYLKENVWVTTSGMQWAPAILFSQQ
                     VLGVDRVLYAMDYPYQFVPEEVKVTDELPISDADRKKLYQTNAEQVFRL"
     gene            complement(2406..3353)
                     /locus_tag="VIG70_04455"
     CDS             complement(2406..3353)
                     /locus_tag="VIG70_04455"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249835.1"
                     /translation="MVRFAFLLALAAAAPHAVSQDYPAREIRSICNFAPGSGADIIVR
                     YYSDQLARLAGKPVIVENKPGAQGTIATAYVAKQPADGYTIHITPASSTLATAPHIFK
                     QLPFDPLRDFAPVTTINSLTFVVAVDAAKPLESINDLIAHLKQKPGHGFYGTQSNSGQ
                     VAAELFKSKTGLDTVYVPFKVTGDAFANLLSGQIDFMSVDSTWARGQSKVRILAVTAA
                     KRSSTMPEIPTLAEAGVAGVDIAPWWGVVVPAGTPRPVIDKLAGWFNQITASSETRQF
                     LARAALDPFPGSPEQMSALLKTELDRWGGFVKLAKIQPQ"
     gene            complement(3384..5984)
                     /locus_tag="VIG70_04460"
     CDS             complement(3384..5984)
                     /locus_tag="VIG70_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249836.1"
                     /translation="MELNQAIQSLRQAKLDEREAAAVVADKKAPAQYKWWQQGIWFGV
                     EVLRYAWLNIYPLLALLVGVVFVFYVKQTREILVDLQQQRLTAAVLAAWAASIWYSMR
                     VLSSTDYPGDADPHPAAKAIMPWLYGQAPRVAPFAGLAVIACSSSIFLTDNPGPSWIA
                     PLAVGVVVVTWAAAWFCDGVIGLVSPPLEPTRVYRWSTLVIAVGAGLIASISWSTVPQ
                     PMRDAPQPLHLEDWLLWLCVALILIPLLVRRRGRLAQWSMAASFAIWLWIVYQTVQSH
                     PGAPRMPFLVLAFAGFGLWFTLRRRELFGITQQAAKPEVEVGRSTFVALGVALFLQLV
                     MVIALTLSPITIGMWIGTLGLLFLGLALLPFFGIVWVFVPKYLTLPSLALVPLIWGIP
                     LGNQPDYLLRDSWFAHQAPERPQLAHHFEQWRAQLPKPDESPVFFVAAAGGGLRAAYW
                     TATMLAAADDHTCGEFGRHVYAYSGVSGGSLGIAAYLAQRQVWEAKSPPPSERCQTGR
                     RQEMADLLRRDFLAPVAGSMLFAEMTQRYVPFDYLEEDRGSILARAWSKAWDDVFPAD
                     KGRFDRPFLDVFASVKDGRAGPAVFLNATAVETGRRAIATNVRVAFPAAIDIFRPVQK
                     AALKTDGLPLREAVLNSARFTYVSPAGTVHSCKQPATDGSCAVEAKLWDRLVDGGYFE
                     NSGVATLTDVMRALATRAKTPGKDQMFVIVIDNSNESELVCRKGRAPERDGNDSLQAG
                     VAPLSGITAPIEALLHVREARGQLEVRRLRMDFSCAEGRLVDWNLFGDQADRSAAQAA
                     GQEPALGWFLSRRSAQWIGQRANEVAQDFPFRHAACHTGKPAGPVRAVVGDPAQPNVV
                     CIEPAKSLKP"
     gene            6086..8659
                     /locus_tag="VIG70_04465"
     CDS             6086..8659
                     /locus_tag="VIG70_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY8249837.1"
                     /translation="MPAALSKADLFARLEQGHAALVTVVTPNKRLAQALQAEFDAFQI
                     KKNLSVWEAPDILPFGAFVERLWEDALYSDLGESLPLLLTPAQEQHLWQEILKEFNFL
                     LKDGAAQQCREAWRLMHQWRIGTGTGTEDAQAFASWSKKYQRSVGGDIDAARLPDLMA
                     QHLDKLKKPKLLVAYAFDVMPPQTREFLEKFEFASCTPGSVPGECARASFPSAKHEIE
                     AAAKWARARLEEGRARIGVVIPELSLHRSQVVRVFSRVMQPDYNLPGAPRTPMPFNVS
                     LGKPLSSFPLVHFALDLLELAFQSVEFSRASHLVRSPFLGRADTELARRGALDARLRK
                     DADNTITLGKLIGSAEAVPLLRKQLEKVFEITRTRPESPAEWARHFSALLDAAGFPGE
                     RALDSDEFQTRAKWHEALGELSKLERLTANFSFAQALAFLKRHCADTLFQPESPDAPI
                     QILGVLESAGLRFDCLWVSGLTDEAWPLDARPNPFIPIAAQKKAGIPQASAEASAELD
                     RRITDEWKRSAAEVVFSYPRKEEDRDFAPSPLIVDVVEKPVAIPDFPRYRDLIFQSKQ
                     LESFADAKAPPVPPGKVRGGTRVLADQAACPFRAFAKWRLGAEPLEEPTAGLDPRDRG
                     KLMHALMKELWVRVKGSSSLGTNLAPAITQAAAAAVKEMGLEGRFAELERERLAKLAR
                     EWLEVERLRAPFKVSSTEQQRSIVLAGLEFQSRIDRMDELERGGHVLIDYKGSRMLSP
                     KDWDGPRPDDPQLPLYAVAAPEELAAVVFAKVLPGEMRFMGYSQAESLLPKVQVYRDW
                     KKLLAQWKQDAEALATSFAAGDARVDPKYELKTCRRCDLQTLCRVYEKFSVLKDEGTR
                     S"
     gene            8743..>9001
                     /locus_tag="VIG70_04470"
     CDS             8743..>9001
                     /locus_tag="VIG70_04470"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEY8249838.1"
                     /translation="MSSRPHTREAKATGRNPATHVVAFQFPAFRPYSRTGEAAAVDHA
                     LTQEDLEPLIHAIRGQRVVLDADLARLYGVGTKALNQAVKRI"
BASE COUNT         1532 a         3011 c         3048 g         1410 t
ORIGIN      
        1 cggccgagaa gttgcacacc gagcggatct cgcggaccgg atagtcctgc cccagcgccg
       61 gaatcgcggt cagcgccgcg atgaatagga ttagagtcct catatggcaa ccatgttacg
      121 ccgggtcgcg gtgctgggcg gcgttcgcct ccccttcgcg cgcgcgaacg gcgcgtacaa
      181 cgagctcagc aaccagcaca tgctcaccgc cgcgatggcg gcgctcgtcg agcgcttcca
      241 gctgaagaat cagaagatcg gggaagtggc ggcgggcgcg gtgatcaagc actcgcgcga
      301 ctggaacctg gcgcgcgaat gcgtcctcgg cagcgggttc gatcccgcca cgccggcgta
      361 cgacctgcag cgcgcctgcg gcacgagcct ctctgccgtg gcgcagctcg cgaaccgcat
      421 cgcccagggc gagatcgact cgggcgtggc cggcggcgcc gacagcgcga gcgacatccc
      481 gctctcgtac agtcctgggc tgcagcgcgc cgtcctttcc gctgcccgga acaagtcgtg
      541 gaccggcaag gtcgcgagca tcggcagcct tcggccgcgc gacctcgcgc ccgccgcgcc
      601 cggcatcgcc gagccgcgca ccggcctgtc gatgggccag cattgcgagg agatggcgaa
      661 ggaatgggcg atcgggcgcc gggagcagga cgagctcgcg ctcgccagcc acaagaacgc
      721 cgcggccgcg tggcgggatg gtttcttcga tgatctgacc gttgaatttt ccggattgaa
      781 aaccgataac aacgtgcgcg gcgacacctc gctcgagaag ttgagctcgc tgagaaccgc
      841 gttcgatccg agccccgcgg gtacgctcac ggcgggcaac agctcgccgc tcaccgatgg
      901 cgcagcctgc gttcttctcg ggtcggaaga atgggcgcgt tcacacggcc tggaagttca
      961 atgtttcatc acccattccg aaaccgctgc ggtcgacttc gtcggcatga acggcgcgaa
     1021 ggaaggcatg ctgatggcgc cggcgtacgc cgtgccgcgc atgatcgacc gcgccggcct
     1081 gaagctgcag gacttcgaca tctacgagat ccacgaggcg ttcgctgcgc aggtgctgtg
     1141 cacgctgaaa gcttgggaat ccgaagagta ttgccgtagc aagttgaagc gtgcgggcgc
     1201 actcggacgc gttgaaagaa ccaagctcaa ccccaaaggc tcgagcgtgg cgctcggcca
     1261 tccattcgcc gcgaccggcg cgcgcatcgt cgccacgctc gcgaagcagc tcgcccagcg
     1321 cggctcgggg cgcgggctga tctcgatctg cgccgccggc gggatgggcg tgaccgcgat
     1381 cctggaacgc tagagccgga agacctgctc cgcgttggtc tggtagagct tcttgcgatc
     1441 ggcgtcgctg atcggcagct cgtcggtgac cttcacttct tccggcacga actggtacgg
     1501 atagtccatc gcgtagagca cgcggtcgac cccgagcacc tgctgcgaga agaggatcgc
     1561 cggcgcccac tgcatgccgc tggtggtgac ccagacgttt tccttcaggt agtcgctggg
     1621 ctttttcttg agagcctgaa ctccgggata gcgcttcgcc gccaccatcg ctcgatgcat
     1681 gaagtcgatg cggaacagcc agaacggcag tccctcgccg aggtgcccga cgaccatccg
     1741 cagctttgga aagcggtcga acacgccggc cacgacgatg cgcagcaggt gcagcccggt
     1801 ctccaccgcg aagccgtaga tcgcgccgtc gaggccgcgc ggcaggaacg ggtcgatcat
     1861 cgccggcggc ggcgtgttgg gatggaggta gagcggcacg tcgagcgcct cggccgcctc
     1921 gaagatctcc cagtacttcg ggtcgtcgag gtattcgccc tgggtgtgcg agttgatcac
     1981 cgcgcccttg aatcccagct tttttacgga acgttgaagc tcggcggccg cggcgttcgg
     2041 ggcttgcggc gcgatcgcgg cgagcgctgc gtagcgctcg gggtattgcc ggatcttctg
     2101 caatagctgg tcgttgaggt ccgtgctgag cgaaaccgcc gtggccgaat cgaagacctg
     2161 cacgcccggc gcgctaagga acacgagctg cctggcgatc ccggtcgcat ccatgtcggc
     2221 gatgcgctgc gcgccgaggt cctggatgcg cgcgacgagc tgcgtcgcgc gcggcgtcgc
     2281 gccgagaaag aacccccaca ggctgcggaa ccccgggtcc caagacgcag gtcgttgttc
     2341 aagtaatttt ttgtaccgcg aaagcaactc ggaaggagcc caggcctcct ccgccgcaat
     2401 tcgaatcact gtggctggat cttggcgagc ttgacgaagc cgccccagcg gtcgagctcg
     2461 gtcttgagga gcgcgctcat ctgctcgggc gatccgggga aggggtcgag cgcggcgcgc
     2521 gcgaggaatt gccgggtctc gctgctcgcg gtgatctggt tgaaccagcc ggcgagcttg
     2581 tcgatgaccg gccgcggggt gcccgcggga accaccacgc cccaccacgg cgcgatgtcg
     2641 acgccggcga cgccggcttc ggcgagggtc gggatctcgg gcatggtgga cgagcgcttg
     2701 gctgcggtga cggcgaggat gcgcaccttc gactggccgc gcgcccaggt cgagtccacc
     2761 gacatgaagt cgatctggcc ggacagcagg ttggcgaacg cgtcgcccgt caccttgaac
     2821 ggcacgtaca cggtgtcgag gccggtcttc gacttgaaga gctcggcggc gacctggccg
     2881 ctgttcgact gcgtgccgta gaagccgtgg ccgggcttct gcttcaaatg ggcaatcagg
     2941 tcgttgatcg attcaagggg tttcgcggca tcgaccgcca ccacgaaggt gagcgagttg
     3001 atggtggtga ccggggcgaa gtccctgagc gggtcgaacg gcagctgctt gaagatgtgc
     3061 ggcgcagtgg cgagcgtcga gctcgccggg gtgatatgga tggtgtagcc gtcggccggc
     3121 tgcttggcga cgtacgcggt ggcgatggtg ccctgcgcgc cgggcttgtt ctcgacgatc
     3181 accggcttgc cggcgagcct ggcgagctgg tcgctgtagt agcgcacgat gatgtcggcg
     3241 cccgagccgg gcgcgaagtt gcagatcgag cggatctcgc gcgccgggta gtcctgcgaa
     3301 accgcgtgcg gcgccgcggc cgcgagcgcc agcaagaaag cgaaccgcac catagctaga
     3361 ctcctcctcg cgggtagtct agctcagggc ttgaggctct tcgccggctc gatgcagacc
     3421 acgttcggct gcgccgggtc gccgaccacg gcgcgcaccg ggccggcggg cttgccggtg
     3481 tggcaggcgg cgtggcggaa ggggaaatcc tgcgccacct cgttcgcgcg ctggccgatc
     3541 cactgcgccg agcgcctgga gaggaaccag ccgagcgccg gctcctgtcc cgcggcctgc
     3601 gccgcgctgc ggtccgcctg gtcgccaaac aggttccagt cgacgagcct cccttcggcg
     3661 cagctgaagt ccatgcgcag ccggcgcacc tcgagctggc cgcgagcttc gcgcacatgc
     3721 agcagcgcct cgatcggcgc ggtgattccg gagagcggcg ccacgcccgc ctgcaggctg
     3781 tcgtttccgt cgcgctcggg cgcgcggccc ttgcggcaga cgagctcgct ctcgttcgaa
     3841 ttgtcgatca cgatcacgaa catctggtcc ttgcccggag ttttcgcgcg cgtggcgaga
     3901 gcgcgcatca cgtcggtcag cgtggcgacg ccggaattct cgaagtagcc gccgtcgacc
     3961 aggcggtccc agagtttcgc ttccaccgcg cagctcccgt ccgtggccgg ctgcttgcaa
     4021 ctgtgcacgg tgccggccgg gctcacgtag gtgaagcgcg cggagttgag caccgcttcg
     4081 cgcagcggca ggccgtcggt cttgagcgcc gctttctgta ccgggcggaa gatgtcgatg
     4141 gccgccggga aggcgacgcg cacgttggtg gcgatcgcgc gccgcccggt ctcgaccgcg
     4201 gtggcgttga ggaacaccgc cgggcccgcg cgcccgtcct tcacgctggc gaacacgtcg
     4261 aggaaggggc ggtcgaagcg gcccttgtcg gcgggaaaga cgtcgtccca ggccttgctc
     4321 caggcgcgcg cgaggatcga gccgcggtcc tcctcgaggt agtcgaacgg cacgtagcgc
     4381 tgcgtcatct cggcgaacag catcgagccc gccaccggcg cgaggaagtc gcgccgaagg
     4441 aggtcggcca tctcctgccg gcggccggtc tggcagcgct cggaaggcgg gggcgacttc
     4501 gcctcccaca cctggcgctg cgcgaggtag gcggcgatgc cgagcgagcc gcccgagacg
     4561 cccgagtacg cgtacacgtg gcgcccgaac tcgccgcagg tatggtcgtc ggccgccgcg
     4621 agcatggtcg cggtccagta cgcggcgcgc agcccgccgc cggcggcggc gacgaagaac
     4681 accgggctct cgtcgggctt cggcagttgc gcgcgccatt gctcgaagtg atgcgcgagc
     4741 tgcgggcgct ccggcgcctg gtgcgcgaac caggagtcgc gcagcaggta gtcgggctgg
     4801 ttgccgagcg ggatacccca gatgagcggc acgagcgcga gcgaaggcag ggtcaggtac
     4861 ttcggcacga atacccagac gatgccgaag aacgggagca gcgcgagccc aaggaacagc
     4921 agtccgagcg tgccgatcca catgccgatc gtgatcggcg acagagtgag cgcgatcacc
     4981 atcacgagct gcaggaagag cgcgacgccg agcgcgacga aggtggagcg gccgacctcg
     5041 acttcgggct tcgccgcctg ctgcgtaatg ccgaagagct cgcgcctgcg cagcgtgaac
     5101 cagagcccga agcccgcgaa cgcgagcacg aggaaaggca tcctcggggc gccgggatgg
     5161 ctctgcacgg tctggtagac gatccacagc cagatcgcga acgaggcggc catcgaccac
     5221 tgggcgagcc tgcctcgacg gcgcaccagc agcggtatga gtatcagcgc cacgcaaagc
     5281 caaagcagcc agtcctccag gtgcagcggc tgcggcgcgt cgcgcatcgg ctgcggcacc
     5341 gtggaccatg aaatcgacgc gatcagcccc gcgccgaccg cgatcacgag cgtgctccag
     5401 cgatagacgc gcgtcggctc gagtggcggc gagaccaggc cgatgacgcc gtcgcagaac
     5461 cacgccgctg cccatgtcac caccaccacg ccgacggcga gcggcgcgat ccagctcggg
     5521 ccgggattgt cggtgaggaa gatcgaagac gagcaggcga tcaccgcgag gccggcgaac
     5581 ggcgcgacgc gcggcgcctg gccgtacagc cacggcatga tcgccttcgc cgccggatgg
     5641 ggatcggcgt cgccgggata gtcggtgctc gatagcacgc gcatcgagta ccagatcgac
     5701 gccgcccagg cggcgagcac cgcggcggtc agccgctgct gctgcaggtc gacgaggatc
     5761 tcgcgcgtct gcttgacgta gaacacgaac accacgccca ccaggagcgc gagcagcggg
     5821 tagatgttga gccaggcgta gcgcaggacc tcgaccccga accagatacc ctgctgccac
     5881 cacttgtact gcgccggggc tttcttatca gccacgacgg cggcggcttc acgctcgtcg
     5941 agcttcgcct ggcgcagcga ttggatcgcc tggttgagtt ccatcgctcc ctcccgaatt
     6001 gccggacgat cataggcttc ggcttccggc gcggccttat cccgcagtcc tgaagacgag
     6061 gtgattagtg cactaaactc gccgcatgcc ggcggcgctt tccaaagctg atcttttcgc
     6121 ccgcctcgag cagggccacg cggcgctggt cacggtcgtc acgccgaaca agcgccttgc
     6181 gcaggccctg caggcggaat tcgatgcctt tcaaatcaaa aagaatctca gcgtctggga
     6241 agcccccgac atcctgccct tcggcgcgtt cgtcgagcgc ctgtgggaag acgccctcta
     6301 ttcggacctg ggcgaaagcc tgccgctcct gctcacgccc gcgcaggagc agcacctctg
     6361 gcaggagatc ctcaaggagt tcaatttcct tctgaaagac ggcgcggcgc agcagtgccg
     6421 cgaggcctgg cgcctgatgc accaatggcg catcggcact ggcacgggca ccgaagatgc
     6481 gcaggccttt gcttcgtggt cgaagaaata ccaacgcagc gtcggcgggg acatcgacgc
     6541 tgcgcgcctg cccgacctca tggcgcagca cctcgacaag ctgaagaagc ccaagcttct
     6601 ggtggcctac gccttcgacg tcatgccgcc gcagacgcgc gagttcctcg agaagttcga
     6661 gttcgcctcc tgcactccag gctccgtccc cggggaatgc gcgcgggcat cgttcccgtc
     6721 agccaagcac gagatcgagg ccgcggcgaa atgggcgcgc gcgcgcctgg aggaaggaag
     6781 ggcgcgcatc ggcgtcgtca tcccggaact gtctctgcac agatcccagg tcgtccgggt
     6841 cttctcccgc gtgatgcagc ccgactacaa ccttcccggc gcgccgcgga ccccgatgcc
     6901 gttcaacgtc tcgctgggca agcccttgtc gtcatttccc ctggttcatt tcgccctgga
     6961 cctgctcgag ctcgcgttcc aaagcgtgga attcagccgc gcgagccacc tcgtgcgctc
     7021 gcccttcctc ggccgcgccg acaccgagct cgcgcgccgc ggcgcgctcg acgcgcggct
     7081 gcgaaaggac gcggacaaca ccattacatt ggggaaactc attggctctg ctgaagcggt
     7141 tcctctcctg cgaaagcagc tcgagaaggt gttcgagatc acccgcaccc ggcccgagtc
     7201 gcccgccgaa tgggcgcggc acttctcggc gctgctcgac gccgccggct tccccggcga
     7261 gcgcgcgctc gactccgacg agttccagac gcgcgcgaag tggcacgaag ctttgggtga
     7321 attatcaaaa ctggaaaggc tgaccgcgaa tttctcgttc gcccaggcgc tcgcattcct
     7381 caagcgccac tgcgccgaca cgctgttcca gcccgaatcg cccgacgcgc cgatccagat
     7441 cctcggcgtg ctcgagtcgg cgggcctgcg cttcgactgc ctgtgggtga gcggcctgac
     7501 cgacgaggcc tggccgctcg acgcgaggcc gaaccccttc atcccgatcg cggcgcagaa
     7561 gaaggccggc atcccgcagg cgagcgcgga agcctcggcg gagctcgacc gtcgcatcac
     7621 ggacgagtgg aagcgctccg ccgccgaagt ggtcttctcc tatccgcgga aggaagagga
     7681 ccgcgacttc gctccttcgc ccctcatcgt cgacgtcgtg gagaagcccg ttgccatccc
     7741 cgatttcccg cggtaccggg atctcatctt tcaatcaaaa cagctggaga gcttcgcaga
     7801 cgccaaagct ccccccgttc cgcccggcaa ggtgcgcggc ggcacccgcg tgctcgccga
     7861 ccaggccgcg tgcccgttcc gcgccttcgc caagtggcgc ctcggcgccg agccgctcga
     7921 ggagcccacc gccggcctcg acccgcgcga ccgcggcaag ctcatgcacg cgctgatgaa
     7981 ggaactatgg gttcgtgtga aaggttcttc ttctctcggg acgaacctcg ccccggccat
     8041 cacccaggcc gccgccgcgg cggtgaagga gatgggcctc gaaggccgct tcgccgagct
     8101 cgagcgcgag cgcctggcga agctcgcgcg cgagtggctc gaggtcgagc ggctgcgcgc
     8161 gcctttcaag gtttcatcca cggagcaaca acgaagcatc gtcctggcgg gactggaatt
     8221 ccagtcgcgc atcgaccgca tggacgagct cgagcgcggc gggcacgtgc tgatcgacta
     8281 caagggctcg cgcatgctct cgccgaagga ctgggacggg ccgcgccccg acgatccgca
     8341 gcttccgctc tatgccgtgg ccgcgcccga ggagctcgcc gccgtggtgt tcgccaaggt
     8401 gcttcccggc gagatgcgct tcatgggcta ttcgcaggcc gagagcctcc tgcccaaggt
     8461 ccaggtctac cgcgactgga aaaagctgct cgcgcagtgg aagcaggacg cggaggcgct
     8521 ggcgacctcg ttcgccgccg gcgacgcgcg cgtcgacccg aagtacgagc tcaagacctg
     8581 caggcgctgc gacctgcaga cgctgtgcag ggtgtacgag aagttttcag tattgaagga
     8641 tgaggggacg agaagttgaa ctcgatgcta gtgcctgcta acgagcaacc ctttgatcgt
     8701 tccacgctca actctgcccc ggcgaactca tcacgatctg tgatgagttc caggccacac
     8761 acccgggagg cgaaagccac cggaagaaac cccgcaactc atgtcgtcgc ctttcagttc
     8821 ccggcgttta gaccttattc gcgcacaggg gaggcggctg cagtggacca cgcactaacg
     8881 caggaggatc tcgaaccgct aatacatgcc atccgcgggc aacgagtcgt actggacgcc
     8941 gatttggcgc ggctctacgg cgtgggcacg aaagcactga accaggccgt caagcgaatt
     9001 g
//
LOCUS       DATBSZ010000069         6552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1239686, whole genome shotgun sequence.
ACCESSION   DATBSZ010000069 DATBSZ010000000
VERSION     DATBSZ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239686"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VIG70_04475"
     CDS             <1..221
                     /locus_tag="VIG70_04475"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEY8249839.1"
                     /translation="MLGTITQNQETFALVRAGPNLYRVRKGNYMGQNFGVITAIDEAQ
                     ISLKELVQDSTGDWVERQSALQLVEAKR"
     gene            218..2326
                     /gene="pilQ"
                     /locus_tag="VIG70_04480"
     CDS             218..2326
                     /gene="pilQ"
                     /locus_tag="VIG70_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEY8249840.1"
                     /translation="MNTKATPRISALLLWLAGLALFATTAAAQNSIDSFEVRQEAGRT
                     VVRVTTKEPLKSVPPNFSVATPARIAFDFPNTVNALGRSNQDIGQGELRSMNIVQGAE
                     RTRMVLNLRSPRGHEAVLDGNTLVITLAEAAVAQTGPAATAQFAEGRGDVKHAVRDID
                     FRRGRGGEGRVVVDLSDTTTGIDIRVQGQNIVVDFLKTSLPENLRRRLDVIDFGTPVN
                     TVSTFQQADNVRMVIEPKGQWEHNAYQSDTQFVLEVKPIVADPSRASAQRGRYTGERL
                     SLNFQNVEVRAVLNVIADFTDLNIITSDTVSGNITLRLRDVPWDQALEIILQTRGLDS
                     RRSGNVIWIAPRDELATREKLQLEAANQISDLEQTRTESFQMNYQKAADVQKLLSDPT
                     QRILSKRGSAVVDARTNTLFVQDTPSRLEEVRRLIAKIDVAVRQVMIEARIVEASDTF
                     SRNLGARIALNDRQASGHRVLGQNSPRVIAGGGLASEGFMTTQIETQPNFFGDTTNVN
                     LPAVGLNNFGAGQVSLILFNSAASRFLNLELSALEADGKGKIISSPRVLTADQVEALI
                     EQGAEIPYQTATSSGATALSFRKAVLSLKVKPQITPDGNVIMTLDVNKDAPGQQVPGG
                     VQINTKHVKTEVLVENGGTVVIGGIYEQNDRTDVTQVPWFGDLPVIGWLFKNKATTNA
                     KTELLVFVTPRIVNERLTIR"
     gene            2396..2929
                     /locus_tag="VIG70_04485"
     CDS             2396..2929
                     /locus_tag="VIG70_04485"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY8249841.1"
                     /translation="MQHAANLYLVDMMGTGKTTVGRTLARRLKLRFVDSDHEIEARCG
                     VKIPVIFEIEGERGFRGRETQAIAELTALDGIVLATGGGVVLAEENRSRLAARGTVVY
                     LRATPEHLYERVRQDRNRPLLAGGDPLGRLRELYRERDPLYSALADLVVDTGRQSVQV
                     LARGLLEQLGARWKASA"
     gene            2911..3993
                     /gene="aroB"
                     /locus_tag="VIG70_04490"
     CDS             2911..3993
                     /gene="aroB"
                     /locus_tag="VIG70_04490"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY8249842.1"
                     /translation="MESLSVALGSRAYPIHIGAGIIGSDGLYQPHLAGGSAAIVSNQV
                     VAPLYLDKVKRALVAAGARHTAIIVPDGEQTKSWQTLNQIFDALLGARCGRDTLIVAL
                     GGGVVGDLAGFAAAVYQRGVPFIQVPTTLLAQVDSSVGGKTAINHALGKNMIGAFHQP
                     RAVISDVATLDSLPERELRSGIAEVIKHGFALDAGFVEWLESNIEKILKKDRAALVHA
                     VRRSCELKARIVAEDERESGARALLNFGHTFGHAIEAGTGYGAWLHGEAVAAGMVMAA
                     ELSQLMGHLKKTEVVRVRDLLKRAGLPVAGPALAPERLMELMALDKKAAKGRTRFVVL
                     EALGRAALRADIDEAAVRGAIVAAAQ"
     gene            4016..>6552
                     /locus_tag="VIG70_04495"
     CDS             4016..>6552
                     /locus_tag="VIG70_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEY8249843.1"
                     /translation="MSLRLDPPEQGLYNPANEHDACGVGFVAHIKGKKSHAIVEQGLS
                     VLKNLSHRGAVGYDPKLSDGAGCLIQIPDRFYREEMAKQGVKLPPAGQYGVGMVFLPR
                     DPASRIACEYEIERAIKDEHQMLLGWRDVPVDNSDLAVPAKQLEPVIRQVFIGRGRRL
                     TVTDALERKLYIIRKSSGHAIQALKLKHGREFYVPSMSARTVCYKGMLLAHQVGEYYL
                     DLKDARVESALALVHQRFSTNTFPSWDLAHPFRLICHNGEINTLRGNVNWIRARQGAI
                     SSPILGGDLAKIWPLIYDGQSDSASFDNALELLVMGGYSVAHAMMMMIPEAWENHTLM
                     DPARRAFYEYHAAMMEPWDGPASIAFTDGRQIGATLDRNGLRPSRYIVTDDDLVIMGS
                     ECGCLPIPEERIVKKWRLQPGKMFLVDVEKGRIVDDKELKETLANAKPYADWIERIRV
                     RLDEVDTEKTRPLKSEVPLLDRQQAFAYTQEDLKFQLAPMAQAGEEPVGSMGNDASLA
                     VLSRRNKTLYHYFKQLFAQVTNPAIDPIREELVTSLVSFIGPKPNLLGIDELNPPLRL
                     EVSQPVLDFYEMEKIRHIERYTGGKFKACELDITYPVAWGKEAIEARLASLAAQAEDA
                     VRSGYSIIVISDRLVDRERVAIPALLALSAIHQHLVNKGLRTSTGLVVETGSAREVHH
                     FALLGGYGAEAVHPYLALETILSFSSDKTKAVKNYVKAIGKGLKKVMSKMGISTYMSY
                     TGAQQFEAVGLEKSLVDKYFTGTASNVQGIDLFEVAEEALRTHRAAFGEKDPVLAAAL
                     DAGGEYAWRVRGEDHTWTPDAIAKLQHASRQNSFQTYKEYAALINDQA"
BASE COUNT         1205 a         2214 c         2149 g          984 t
ORIGIN      
        1 agatgctcgg caccatcacc cagaaccagg agaccttcgc gctggtcagg gccggcccga
       61 acctgtaccg ggtgcggaag ggcaactaca tgggccagaa cttcggcgtg atcaccgcga
      121 tcgacgaggc ccagatcagc ctcaaggagc tggtgcagga ctcgaccggc gactgggttg
      181 agcgccaaag cgcgctgcaa ttggtggagg caaaacgatg aataccaaag ccactccccg
      241 catttctgcg ctcctgctct ggctggcagg gctcgccctg ttcgcaacca cggcggccgc
      301 gcagaacagc atcgacagtt tcgaagtccg ccaggaggcc ggcaggacgg tggtgcgcgt
      361 cacgaccaag gagccgctga agagcgtgcc gccgaacttc tcggtcgcca cgccggcgcg
      421 catcgcgttc gactttccca ataccgtgaa cgcgctgggc cgctccaacc aggatatcgg
      481 gcagggcgag ctgcgcagca tgaacatcgt gcagggcgcc gagcgcacgc ggatggtgct
      541 caacctgcgc agcccgcgcg gccacgaggc agtgctcgac ggcaacacgc tggtcatcac
      601 cctcgcggag gcggcggtgg cgcagaccgg accggcggcg acggcgcagt tcgccgaggg
      661 ccgcggcgac gtcaagcacg ccgtgcgcga tatcgacttc cgccgcggcc gcggcggcga
      721 ggggcgcgtg gtggtcgatc tttcggacac caccaccggc atcgacatcc gcgtgcaggg
      781 gcagaacatc gtggtcgatt tcctcaagac ctcgctgccc gagaacctgc ggcgccggct
      841 cgacgtgatc gacttcggca cgccggtcaa tacggtgagc acctttcagc aggcggacaa
      901 cgtgcgcatg gtgatcgagc cgaaaggaca atgggagcac aacgcttacc agtccgacac
      961 gcagttcgtg ctcgaggtga agccgatcgt cgccgatccg tcgcgcgcca gcgcgcagcg
     1021 cgggcgctat accggcgagc ggctctcgct caacttccag aacgtggaag tgcgcgcagt
     1081 gctgaacgtg atcgccgact tcaccgacct gaacatcatc accagcgaca cggtcagcgg
     1141 caatatcacg ctgcggctgc gagacgtgcc gtgggaccag gcgctcgaga tcatcctgca
     1201 gacgcgcggc ctcgacagcc ggcgcagcgg caacgtgatc tggatcgcgc cgcgcgacga
     1261 gctcgccacg cgggagaagc tgcagctcga ggcggcgaac cagatctctg atctggagca
     1321 gacccgcacc gagtcgttcc agatgaacta ccagaaagcg gccgacgtgc agaagctgct
     1381 gtcggacccg acgcagcgca ttctctccaa gcgcggcagc gcggtggtcg atgcccgcac
     1441 caacacgctg ttcgtgcagg acacccccag ccgcctcgag gaagtgcgcc gactgatcgc
     1501 gaagatcgac gtcgcggtgc gccaggtgat gatcgaggcg cgcatcgtag aggcgagcga
     1561 cacgttcagc cgcaacctcg gcgcgcgcat tgcgctgaac gaccgccagg cctccggcca
     1621 tcgcgtcctg ggacagaaca gcccgcgcgt catcgccggc ggcggcctgg cgagcgaggg
     1681 tttcatgacg acccagatcg aaacccagcc gaacttcttc ggcgacacga ccaacgtcaa
     1741 cctgccggcc gtcggcctca acaacttcgg cgcgggacag gtctcgctta tcctgttcaa
     1801 cagcgcggcg agccgcttcc tcaacctcga gctctcggcg ctggaggccg acggcaaggg
     1861 caagatcatc tccagcccgc gtgtgctgac cgccgaccag gtcgaggcgc tgatcgagca
     1921 gggcgccgag atcccgtatc agaccgcgac ttcgtcgggc gcgaccgcgc tctccttccg
     1981 caaggcggtg ttgtcgctga aggtgaagcc gcagatcacc cccgacggca acgtcatcat
     2041 gacgctcgac gtaaacaagg acgcgcccgg ccagcaagtg cccggcggcg tgcagatcaa
     2101 caccaagcac gtgaagaccg aggtcctggt cgagaacggc ggcacggtgg tgatcggcgg
     2161 catctacgag cagaacgacc gcaccgacgt cacccaggtg ccgtggttcg gcgacctgcc
     2221 cgtcatcggc tggctgttca agaacaaggc cacgacgaac gccaagaccg agctgctggt
     2281 gttcgtcacg ccgcgcatcg tcaacgagcg cctcactatt cgctagcacc gcctcttcgc
     2341 ttctctcctc tcgacggccg gtccccccgg ccgtttttgt tcctacaatg ccgggatgca
     2401 gcacgcggca aacctctacc tggtcgacat gatgggcacg ggcaagacca ccgtcgggcg
     2461 cacgctcgcg cggcgcttga agctgcgctt cgtcgactcg gaccacgaga tcgaggcgcg
     2521 ctgcggcgtg aagatcccgg tgatcttcga gatcgaaggc gagcggggct tccgcggccg
     2581 cgagacgcag gcgatcgccg agctcacggc gctcgacggc atcgtgctcg ccaccggcgg
     2641 cggcgtggtg ctcgccgaag agaaccgcag ccggctggcc gcgcgcggca ccgtggtcta
     2701 tctgcgcgcg acccccgagc acctgtacga gcgcgtgcgc caggaccgca accggccgct
     2761 gctcgccgga ggcgatccgc tcgggcgctt gcgcgagctc taccgcgagc gcgatccgct
     2821 gtatagcgcg ctcgccgatc tggtggtcga taccggcagg cagagcgtgc aggtgctggc
     2881 gcgcggcctg ctcgagcagc tcggagcgcg atggaaagcc tcagcgtagc cctcggcagc
     2941 cgggcctatc ccatccatat cggggccggc atcatcggct cggacgggct ttaccagcca
     3001 caccttgccg gtggcagcgc cgcgattgtc agcaaccagg tagtagcgcc gctttacctc
     3061 gacaaagtga agcgagcgtt ggtcgccgcg ggcgcacgcc acacagcaat catcgtcccg
     3121 gacggcgaac agacgaaaag ctggcagacc ctgaaccaga tcttcgatgc gctgctcggc
     3181 gcgcgctgcg ggcgcgatac gctgatcgtt gccctgggcg gcggcgtcgt cggcgatctc
     3241 gccggcttcg ccgctgcggt gtatcagcgc ggcgtgccct tcatccaggt gccgaccacg
     3301 ctgctcgcgc aggtcgattc ctccgtcgga ggcaagaccg caatcaacca cgcgctaggc
     3361 aagaacatga tcggcgcgtt ccaccagccg cgcgcagtga tctcggacgt ggcgacgctc
     3421 gactccctgc ccgagcgcga gctgcgctcc ggcatcgccg aggtgatcaa gcacggcttc
     3481 gcgctcgacg cggggttcgt ggaatggctc gagtccaaca tcgaaaaaat cctgaagaag
     3541 gatcgcgcgg ccctggttca tgcggtccgt cgcagctgcg agctgaaggc gcgcatcgtg
     3601 gccgaggacg agcgcgaatc gggagcgcgg gccttgctca acttcggtca caccttcggt
     3661 cacgccatcg aagccggcac cggctacggc gcctggctgc acggcgaagc cgtcgccgcc
     3721 gggatggtga tggcggcaga gctatctcag ctaatgggtc acttgaaaaa aactgaagtc
     3781 gtccgcgttc gcgacctgct gaagcgcgcg gggctgcccg tggccggccc ggcgctcgcc
     3841 cccgagcgcc ttatggagtt gatggcgctc gacaagaagg cggccaaggg caggacgcgc
     3901 ttcgtcgtgc tggaggcgct cggacgcgcg gcgctgcgcg ccgacatcga cgaagctgca
     3961 gtgcgcggag ccattgtcgc tgcagcgcag taaatgtata ttcagtagcg ccgtcatgag
     4021 cttgcgactc gatccgcccg agcaagggct ctataacccc gcgaacgagc acgacgcgtg
     4081 cggcgtcggc ttcgtggcgc acatcaaggg caagaagtcg cacgcgatcg tcgagcaggg
     4141 cctgtcggtt ctgaagaacc tgagtcatcg cggcgcagtg ggctacgacc cgaagttgtc
     4201 ggatggcgcg ggctgcctga tccagatccc ggaccggttc tatcgcgagg agatggcgaa
     4261 gcagggcgtc aagcttccgc ccgcggggca atacggcgtc ggcatggtat tcctgccgcg
     4321 cgatcccgcc tcgcgcatcg cctgcgagta cgagatcgag cgcgccatca aggacgagca
     4381 ccagatgctg ctcggctggc gcgacgtccc ggtcgacaac tcggacctcg ccgtgccggc
     4441 gaagcagctc gagccggtga tccggcaggt cttcatcggc cgcggccggc gcctgacggt
     4501 gaccgatgcg ctcgagcgca agctctacat catccgcaag agctcgggcc acgccatcca
     4561 ggcgctgaag ctcaagcacg ggagagagtt ctacgtgccg tcgatgtcgg cgcgcaccgt
     4621 gtgctacaag ggcatgctgc tcgcccacca ggtcggcgag tactacctcg acctcaagga
     4681 cgcgcgcgtc gaatcggcgc tcgcgctcgt gcaccagcgc ttctcgacca acaccttccc
     4741 gtcgtgggac ctggcgcacc cgttccgcct gatctgccac aacggcgaga tcaacaccct
     4801 gcgcggcaac gtgaactgga tccgcgcgcg ccagggtgcg atttcatcgc ccatcctggg
     4861 cggtgatctc gcgaaaatct ggccgctgat ctacgacggc cagtccgatt cggcctcgtt
     4921 cgacaacgcg ctcgagctgc tggtgatggg aggctactcg gtcgcgcacg cgatgatgat
     4981 gatgatcccg gaggcgtggg aaaaccacac gctcatggat ccggcgcggc gcgcgttcta
     5041 cgaataccac gcggcgatga tggagccctg ggacggcccg gcgtcgatcg ctttcaccga
     5101 cggccgccag atcggcgcca cgctcgaccg caacggcctg cggccttcgc gctacatcgt
     5161 caccgacgac gacctggtca tcatggggtc ggagtgcggc tgcctgccga tccccgaaga
     5221 gagaatcgtc aagaagtggc gcctgcagcc cggcaagatg ttcctggtcg acgtggagaa
     5281 gggccgcatc gtcgacgaca aggagctcaa agagacgctg gcgaacgcca agccctacgc
     5341 cgactggatc gagcgcatcc gcgtgcggct cgacgaggtc gacaccgaga agacccggcc
     5401 gctcaagtcg gaggtcccgc tgctcgaccg ccagcaggcg ttcgcttaca cgcaggagga
     5461 cctcaagttc cagctcgcgc cgatggcgca ggcgggcgag gagccagtcg gctcgatggg
     5521 caacgacgcc tcgcttgcgg tgctgtcgag gcgcaacaag accctctacc actatttcaa
     5581 gcagctcttc gcgcaggtga ccaacccggc catcgacccg atccgcgagg agctggtcac
     5641 ctcgctggtg agcttcatcg ggcccaagcc caacctcctc ggcatcgacg agctgaaccc
     5701 gccgctgcgc ctcgaagtgt cgcagccggt gctcgatttc tacgagatgg agaagatccg
     5761 ccacatcgag cgctacaccg gcggcaagtt caaggcctgc gagctcgaca tcacctaccc
     5821 ggtggcctgg ggcaaggagg cgatcgaggc gcgtctcgcc tcgctcgcgg cgcaggccga
     5881 ggatgcggtg cgctccggct attcgatcat cgtgatctcg gatcgcctgg tcgaccgcga
     5941 gcgcgtcgcg attcccgcgc ttctcgccct gtccgcgatc caccagcacc tggtgaacaa
     6001 gggcctgcgc acttcgaccg ggctggtggt cgagaccggc agcgcccgcg aagtccacca
     6061 cttcgcattg ctcggcggct acggcgccga ggcggtgcac ccgtatctcg cgctcgagac
     6121 catcctttcc ttctcgtccg acaagacgaa agcggtcaag aactacgtca aggcgatcgg
     6181 caaggggctc aagaaggtca tgtccaagat gggcatctcg acctacatgt cctacaccgg
     6241 cgcgcagcag ttcgaagccg tgggcctgga gaaatcgctg gtcgacaagt acttcaccgg
     6301 cacggcgagc aacgtgcagg gcatcgatct gttcgaggtc gccgaagagg cgctccgcac
     6361 ccaccgcgcg gcgttcggcg agaaggaccc ggtgctggcg gcggcgctcg acgccggcgg
     6421 cgagtacgcc tggcgcgtgc gcggcgagga ccacacctgg acgccggacg cgatcgccaa
     6481 gctgcagcat gcgtcgcgcc agaactcctt ccagacctac aaggaatacg cggcgctgat
     6541 caacgaccag gc
//
LOCUS       DATBSZ010000070        16353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2264009, whole genome shotgun sequence.
ACCESSION   DATBSZ010000070 DATBSZ010000000
VERSION     DATBSZ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16353
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2264009"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VIG70_04500"
     CDS             <1..819
                     /locus_tag="VIG70_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY8249844.1"
                     /translation="AYSNLPVGLNFLLAGYAYSQGELAFDALPLQDARTRVHVLPVGY
                     VRSLDVFGKAGNVALLLPLVDLTGTATLDGASEVRREVSGLGDPTVRLAVNLYGAPAL
                     RPAEFAAWRQDLIVGASLAVTAPFGQYDNDRLVNIGSNRWSIKPELGVSQALGRWTVE
                     LAAGVTWFTRNDEFFNGNTREQDPLYSVQLHLTRQLGRAAWGAFSVTYYDGGRNSING
                     VENTQALGGTRLGATLSLPVDQRNSVRLSAHSGVYARTGSDFDGVSALWQHLWY"
     gene            830..4159
                     /locus_tag="VIG70_04505"
     CDS             830..4159
                     /locus_tag="VIG70_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY8249845.1"
                     /translation="MKRLAYALGALVVLAALVVLVAPAYIDTPAVRAEIQRRLAHAVD
                     GEVTWEALEIGLFPAPHGELRRVRIEMPDRLSAAADDVKAYLRLWPLLRGRAEIASFS
                     VLRPEVRIQASGESEQQELDAVAAYRKVVEPLVQALQRFAPDTQLRIEQAAVDPGGLR
                     ELNANVRTAADGVDLELSAASAWWKRLSIKGRVEYASLAARAQVELDALVVDKDLPPA
                     TIRAQLRTDAKTVVEGDFDASLAAVAAAKGKLLLPAGKTPELSARVDVADLAKALALA
                     RLKVAGLDAIESAEGRLSADVAVSLAAQWQAKIEITKSDAAVKLAQLPWKLSAHSGHV
                     TVTPEQVHVAGARGLVGDSTFENAAARIELGKAPRLSAASGRATVKLEQWLPWLQTKL
                     PLEQIASASGSVDVALKRLALRFDRPAEVDFEAVATPRKVSAKLTALPQPVNLAGGTI
                     HADAKRVRASGLQGSIGDSTFEGVAAEINLGKAPALSAASGRATVKLEQWFPWLQAKV
                     PVEDLTALSGTVEVDVKRVALRFDRPAEVDFDAVATPRNVRAALKLLPGSVSVTGGSL
                     RAGPEKVNLDKLAVEMLDARAQVSGSLSVKNTALELALSGGELGEKAMRWALERSGVP
                     PRFEPKTPLRFAARRIAWAPDEPLEVDARVDFDGGPALGVVLAWKPEALEVRRLTIKD
                     ARSDAVLGATVAGDRIDTSFSGRLHGQSIPAMLRQPLPAASGDARGELRVSIDRKRPE
                     RTTAEGSLHIEALDLSWLAGRRAVVQRADLSTEGDRLRITQARLDVEEQVFDLRGEIR
                     RTDQAPVIEARVESAGVMLDRLLPAPDPSAPKKKSSALWPLPVAGRVEVAAGFIQYKD
                     YRIEPFDGILSLEPKRARLDVKEARMCGVSFPMEVEIQPEDASAAAHITIKNQPLERT
                     LHCLTGGNVQITGNVDLKAELRVQGRRPHLVRGLTGTVETEVRQGRVKKFALLGNILS
                     FRGIASVSEMKEEGFPYRAITAKGHFANGEFLVDESFFDSDAVRLAAQGRVDLLGANS
                     QLTVLVGLLTHVERIAGAIPIIGDVFGGSMIALPMEVRGDIRDPVIVPLGPRAVTNQL
                     LGIFERTLKLPGKLVPAPEAK"
     gene            complement(4280..4852)
                     /locus_tag="VIG70_04510"
     CDS             complement(4280..4852)
                     /locus_tag="VIG70_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249846.1"
                     /translation="MKRTLLAAALLVPALVLAQPRGAIVTDRIEAVVTVTKVDPKART
                     VTMRGPRGNLGVLHVPPEAQNLDRVKVGDRVKVVYAEAAVIALTRGGEPAATVEEEVQ
                     LAPKGGTPGGIKVRTTKISAVVDAIDYKNRYVALRGPKGNTLALPVSDEVKNLESVKV
                     GDKLTIAYTQALALELVVEEKPKKPAAKKK"
     gene            complement(4924..5547)
                     /locus_tag="VIG70_04515"
     CDS             complement(4924..5547)
                     /locus_tag="VIG70_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8249847.1"
                     /translation="MPRETALYDTVPCVRVVDDDAAVLKSLGRLLNAAGFAVRTFPSS
                     QDFLRQHDRDAPGCVVIDLAMPGVDGLQLQQVLARSGDPCPVIFISGYGDVPSSVRAM
                     KAGAVDFLTKPFDQSQFLDAVRTAIDKDRSARAARAERSSVGVRVAGLTPREREVMTH
                     VIAGHLNKQIAAELGIAEKTVKVHRARVMKKMEVGSVADLVWQVRRN"
     gene            complement(5579..5959)
                     /locus_tag="VIG70_04520"
     CDS             complement(5579..5959)
                     /locus_tag="VIG70_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249848.1"
                     /translation="MRLFLGLAAAALVQGCVFYPRTTTVHDEECRIETRRMTLQAEPF
                     GYLASCSGKECASMLVVIGAVTAGSAVVSGSIVVAGNVVYWMERRGKCPARHLDSKIV
                     TASAFLFLFQNDRDVSQARCATKR"
     gene            5992..7125
                     /locus_tag="VIG70_04525"
     CDS             5992..7125
                     /locus_tag="VIG70_04525"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8249849.1"
                     /translation="MAITRPREGLLALLCALALVPLPAAAQTYRSDEHSFRVVKVVEG
                     FEQPWSLAFLPDGRMLVTEKAGRLRIVSQGKLDPKPIEGLPQVTVHGQGGLQDVVLHP
                     DFSKNSLVYLAYAARGSDGVGTELARGRLVGHRLEGVEVLFKQTPKGSRGQHFGGRIV
                     FDRAGYVYLTLGDRGEMPRAQRPDDHAGSVIRLHDDGRLPADNPFAGKPGWKPEKFTL
                     GNRNMQGAALHPQTGMLWTHEHGPQGGDEVNVIRPGVNYGWPVITYGVNYGIGTKIGE
                     GTRKEGMAQPIHYWVPSIAPSGMAFYTGDKFARWKGDLFVGALRDQMLVRLKLDGEKV
                     VSEERLLKGVLGRIRDVRAGPDGFIYVLTDESNGVLARLEPAT"
     gene            7208..7678
                     /locus_tag="VIG70_04530"
     CDS             7208..7678
                     /locus_tag="VIG70_04530"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEY8249850.1"
                     /translation="MNRFRRITLATMLGGLAAGIGFKAFGYSRRHGYGPIDPADLEKR
                     LDRMLKHFYVEIDATEEQKQKLEPIVKQAAKDLMPLREQLHAGRREAIELLSQDRVDP
                     AALEALRGRKIQLADEASRRLTRAIAEAADVLTPAQRKGLATHFARRHGRWGHA"
     gene            7790..8149
                     /locus_tag="VIG70_04535"
     CDS             7790..8149
                     /locus_tag="VIG70_04535"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY8249851.1"
                     /translation="MPHAVSGPLGSAAVLAAVVLFLSSVRALRIAGTPVPGDRPTTAI
                     VRAGPFRYSRNPIYLSFSLLHAGVACWVNSLWLIVTLMPAAALMSFVVIPREERYLEA
                     RFPSEYLPYKAAVRRWL"
     gene            8146..8757
                     /locus_tag="VIG70_04540"
     CDS             8146..8757
                     /locus_tag="VIG70_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8249852.1"
                     /translation="MRAAIILLLSVACAGCGSWTGFGFRADGAEMARLREVLALEAGK
                     VVADVGAGKGQLTLAFAREVGSNGRVFSTEIDAARLKQLRIAVVDARLDNVTVVEALS
                     HETGLPPGCCDAIVLRRVYHHLSDAASINASVLRALRPGGVLAVIDFPPPFFWSRGSL
                     GVPAKTVVTEVAGSGLEPLRLIDDWPGRGPLGSYCAVFRKPRV"
     gene            8768..9451
                     /locus_tag="VIG70_04545"
     CDS             8768..9451
                     /locus_tag="VIG70_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8249853.1"
                     /translation="MERVLLIEDDARLAAMVSEYLGGSGYRVTTAASGASGLERLEKE
                     PYDALVLDLSLPDMDGLEVCRRLRARWELPVLMLTARGDAMDRIVGLEIGADDYLPKP
                     FEPRELLARLKAVLRRANRQGKDSLLRFGRLEIDRDSRAVRVGGKEAALTSFQFTLLV
                     TLAENAGRVLSRDALMNLMKGEKLEAFDRSIDVHVSRIRAAIEDDPKKPRRIITVRGA
                     GYVFAKQQN"
     gene            9538..10695
                     /locus_tag="VIG70_04550"
     CDS             9538..10695
                     /locus_tag="VIG70_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8249854.1"
                     /translation="MRRLYLRIYFAVLASLVAFAVGAGVLWRTLAEPREGTELGVIAR
                     NVLAPAEAAPAEQQAALERVARGARMDLALFSRDGIRLAAVGGALTAPERTGWRHSGG
                     DPSWATQLSDGRWLVSRPHHRRGPAFGVFALALVLVAVGVGAYPVARRLTQRLERLQR
                     GVDALGAGELSARVKVEGRDEVARLAESFNRAAARIEELVGAHKSLLANASHELRTPL
                     ARIRLALELGKDKREIERDIAELEQLIDEVLIASRLDAVTSLENTEEVDLLALAAEEA
                     ARYDVEAQGEPVVIRGDPRLLRRMIRNLLENARRHGAPPIEIHVKGRQLRVCDAGAGV
                     PAAERERIFEPFYSKRGTGLGLALVRQIARRHGGDVRCEGNCFVVTLFIPA"
     gene            complement(10682..11629)
                     /locus_tag="VIG70_04555"
     CDS             complement(10682..11629)
                     /locus_tag="VIG70_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249855.1"
                     /translation="MNRLLAVLTSLWVTTAFAQYPDKPIRLIVPFPPGGAAELGARIF
                     SVPLGQALGQPIVIDTKPGADGAIAADAVMKAAPDGYTLFYATNTAFNWVPATRKNPP
                     YNPVTDFTPVSFVGYFGFFLFSHPSVPAKSVAELLAYARANPGKVNYGSGNSTAQLFG
                     AQLAQLEKLDIVHVPYKGDGPLVIDLLAGRVHIAFSTPGTAAPQVKEGKLRALAAMFP
                     NRSALLPEVPTAAEAGLGKMSLTPWGGVFGPKGMPKEVVDRVARELAVVLKRAEVRDA
                     FGKLAFEPKSSTPEELSAILKQQIEIWGSTAREVGIKPE"
     gene            complement(11639..12433)
                     /locus_tag="VIG70_04560"
     CDS             complement(11639..12433)
                     /locus_tag="VIG70_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8249856.1"
                     /translation="MYRGMDRAALDAAYNNGAAVADSPKIVADWEARSARLRAAHPEG
                     MDLRYGPDERNRIDYFAARRDGPVLAFIHGGYWQNRAKEIFSFVAEGPLAWGINVALI
                     GYTLAPQKRLDGIVAEIRAALTWLHTAIPLLGGEKARLFVSGWSAGGHLTAMAMDHPA
                     VKGGLAISGIYDLEPMRLCYINDKLCLDEAEEKRNSPMFLKAAEKPLFIAYGADELPE
                     LCRQSEEYSKKLSHTTLLAVPQRNHFTVLEELASPHGALTALARKL"
     gene            complement(12433..13425)
                     /locus_tag="VIG70_04565"
     CDS             complement(12433..13425)
                     /locus_tag="VIG70_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8249857.1"
                     /translation="MPALRLTLACWDYDRTRALMDGTVAPDGIELVYLNQPVEETFFR
                     MLRYREFDCSEMSLSSYVASLQAENPPFVAIPVFPSRYFRHSCIFVSAKSGIRKPEEL
                     KGRRIGVPEYQMTAPVWIRGILSDDHGVKVTDVEHYSGGEEEPGRDEKLKLNLPPNIR
                     LKAIPGDKTLSRMLADGELDALVTARSPSTFHKEPDKVKRLFPNYVELEKDYYRRTKI
                     FPTMHTVVIRRDVYAKNPWVAQSLYKAFAEAKAKAYRLYDQTAALPAMVPWLVAELEE
                     ARREMGEDWWPYGLEPNRKVLETFLRYHHEQGLSKRRFAPDELFARETLKSFKV"
     gene            complement(13441..14586)
                     /locus_tag="VIG70_04570"
     CDS             complement(13441..14586)
                     /locus_tag="VIG70_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8249858.1"
                     /translation="MKKLRIGVAGLGRAFAVMAGAFRDPRVELVAAADPRREALAKFK
                     ADHGGRVFTSTEDLCADPGVDVVYIATPHQFHAPHTRLAASHGKHILVEKPMALFLQD
                     CRAMIDVARKAGVALIVGHSHSFDAPILRTRDLIQSGAYGAVRMVTALDYTDFLYRPR
                     RPDELDTEFGGGVVFNQAAHQVDIVRLLAGGNVRSVRAYTGKWDPSRQTEGAYTCLLG
                     FDNGAFASLVYSGYAHFDSDEFMGWIGEMGQERDPRQYGTARRALASGDELAVKNARN
                     YGGPEFKEIPPVAHQHFGVVIVSCEKGDLQPLPTGVMIHGDNERRFDRLPLPEVPRKE
                     VIDELYAAVVEKRAPLHSGEWAMGNLEVSLAILRSAREGVDIPLRNR"
     gene            14603..15892
                     /locus_tag="VIG70_04575"
     CDS             14603..15892
                     /locus_tag="VIG70_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8249859.1"
                     /translation="MLTKEQNQLLCRVEGDAVMGQMSRRHWLPVCMAEEVGQADGAPV
                     RARLLGEDLVVFRDTTGRLGVLGEHCLHRRASLVFGRNEECGLRCLYHGWKFDVEGNV
                     VDMPSEAPGAAARLGKKTKAYPAREGGGFVWIWMGPREEMREFEPPAWAPKPGIRYAI
                     VKMHAACNWAQVLEGSIDSAHSSSLHSTNMPSAEVEGSTATDSAWLRPSTDKAPKLQF
                     EATSYGFRYAAIRKPIRNPETHQYVRTTLYIAPFTCLIPPNDQYNLAQMLIPIDDVNT
                     LFYWIAWHPDPKKGITQQAWRRFCGAEVGVDLDENFRKKRNLDNRYLQDRDAMKRGDW
                     TGIHGIPTQDMAMWESMGPISDRSQDHPGASDLAVAQFRRLVIAAAKKFQEGGPAIGA
                     AEPRVRQVTLASFEGVVPKSVDWRTLGAPQEEPVGAK"
     gene            complement(15908..>16353)
                     /locus_tag="VIG70_04580"
     CDS             complement(15908..>16353)
                     /locus_tag="VIG70_04580"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEY8249860.1"
                     /translation="CIDGVAVEVGRGDDRTSLLRFILTDRWHRAQRPPATEWRLAGYW
                     ELSAGLWDNREESTADVGFTPVFRIERGTLYFEGAVGVHLVQARISAQRSFSTALQFA
                     DHLGAGIHSGKYDFGVRVQHISNGGFHSPNPGINFVTLQVHYNLE"
BASE COUNT         2579 a         5427 c         5684 g         2663 t
ORIGIN      
        1 gcctactcga acctgccggt cgggctgaat tttctgctcg ccggctatgc gtactcgcag
       61 ggcgagcttg cgttcgacgc gctgccgctg caggacgccc gcacgcgcgt ccacgtgctg
      121 ccggtgggct acgtgcgttc gctcgacgtg ttcggcaagg ccggcaacgt cgcgctgctg
      181 ctgccgctcg tcgacctgac ggggacggcg acgctggacg gcgcgagcga ggtacggcgc
      241 gaggtgagcg gcctcggcga tcccacggtg cggctggcgg tcaatctcta cggcgcgccg
      301 gcgctgcgcc cggcggagtt cgccgcctgg cgccaggacc tgatcgtcgg cgcgagcctc
      361 gccgtcactg cgcccttcgg ccagtacgac aacgaccggc tggtcaacat cgggtccaac
      421 cgctggtcga tcaagccgga gctcggcgtg tcgcaggcgc tcggccgctg gaccgtcgag
      481 ctcgcggcgg gcgtcacctg gttcacccgc aacgacgagt ttttcaacgg aaatacccgc
      541 gagcaggacc cgctttattc ggtgcagctg caccttaccc gccagctcgg ccgcgccgcc
      601 tggggcgcgt tcagcgtcac ttactacgac ggcggccgaa actccatcaa cggcgtcgaa
      661 aacacgcaag ctctcggtgg cacgcgcctg ggcgcgacct tgtcgttacc ggtcgaccag
      721 cgcaattcgg tcaggctgtc tgcgcacagc ggagtctacg cacgcaccgg ctccgacttc
      781 gacggcgtca gcgcgctctg gcagcacctc tggtattaga gtaggccgca tgaagcggct
      841 ggcctacgcc ctcggggcgc tcgtcgtcct agcggcgctg gtggtcctgg tcgcgcccgc
      901 gtatatcgac acacccgcgg tgcgcgccga gatccagcgc cggctcgcgc acgccgtgga
      961 cggcgaggtg acctgggaag cgctcgaaat cggcctgttc ccggcgccgc acggcgagct
     1021 gcgccgggtg cgcatcgaga tgcccgaccg cctgagcgcc gctgccgacg atgtcaaggc
     1081 atacctcagg ctgtggccgc tgctgcgcgg ccgagccgag attgcgtcgt tctccgtgtt
     1141 gcggcccgaa gtccgcattc aggcttcagg agaatctgag cagcaggagc tcgacgcggt
     1201 cgccgcctac cgcaaggtgg tcgagccgct ggtgcaggcc ctgcagcggt tcgcgccgga
     1261 tacgcagctg cgcatcgagc aggcggcggt cgacccgggg gggctgcgcg agctgaacgc
     1321 caatgtgcgg accgccgccg acggcgtaga cctcgagctg tcggcggcga gcgcgtggtg
     1381 gaagcgcctc tccatcaagg ggcgtgtcga atacgccagc ctcgcggcgc gcgcgcaggt
     1441 cgagctcgac gcgctggtgg tggacaagga cctcccgccg gctacgattc gagcgcagct
     1501 gcgcaccgac gcgaagaccg tcgtcgaagg cgacttcgat gccagcctcg ccgccgtcgc
     1561 ggcggcgaag ggcaagctcc tccttcccgc tggaaagacg ccggagctga gcgcccgcgt
     1621 cgacgtggcc gatcttgcga aggcgctcgc gctggctcgg ctcaaggtcg cgggcctcga
     1681 cgctatcgag tcggcggaag ggcgcttgtc cgcggatgtg gccgtttccc tggcggctca
     1741 gtggcaagcc aaaatcgaaa tcacgaaatc cgatgccgcc gtaaagctgg cgcagctacc
     1801 gtggaagctg tcggcgcact cggggcacgt cacggtcacg ccggagcagg tgcatgtcgc
     1861 cggagcgcgc ggtctcgtgg gggattcgac tttcgagaat gccgccgccc ggatcgagct
     1921 cgggaaagcg cctcgcctat cggcagcctc cgggcgggca accgtaaagc tcgaacaatg
     1981 gctgccctgg ctgcagacaa agctgccgct cgagcagatc gcttcggctt ccggcagcgt
     2041 cgacgttgcg ctgaagcgcc tcgctctgcg cttcgaccgc ccggcggagg tcgacttcga
     2101 ggctgtcgcc acgccgcgca aggtgagtgc caagctcacg gcgctcccgc agccggtgaa
     2161 cctcgcgggt ggaacgattc atgccgacgc gaagcgcgta cgtgcctcag ggttgcaggg
     2221 ctcgatcggc gattcgacgt tcgagggagt tgctgccgag atcaatcttg gaaaagcacc
     2281 cgcgttgtcg gcggcttccg ggcgagcgac ggtaaagctc gagcagtggt ttccctggct
     2341 gcaggcgaag gtaccggtgg aagacctcac cgccctttcg gggacggtcg aggtggatgt
     2401 gaagcgcgtg gcgctgcgct tcgatcgccc cgcggaggtc gatttcgacg ccgtcgccac
     2461 gccgcgcaat gtcagggccg cactgaagct gctgccgggt tcggtcagcg ttacgggggg
     2521 gtcgttgcgc gccgggcctg agaaagtgaa cctggataag ctcgccgtgg aaatgctcga
     2581 cgcgcgcgcc caggtgtcgg gaagcctctc ggtgaagaat acggcgttgg agctcgcgct
     2641 gtccggtggc gagctcggcg agaaagcgat gcgctgggcg ctggagcgca gcggcgtgcc
     2701 gccgcggttc gagccgaaaa ctccgctgcg ctttgcggcg cggcgcatcg cgtgggcgcc
     2761 ggacgagccg ctggaagtcg acgcgcgcgt cgatttcgat ggcggccctg cgctcggggt
     2821 ggtgctggcg tggaagcccg aggctctgga ggtgcgccgc ctgacgatca aggacgcgcg
     2881 cagcgacgcg gtgctcggcg ccaccgtcgc cggcgaccgg atcgacacaa gcttttccgg
     2941 caggcttcac ggccaaagca tccccgcgat gctgcgccag ccgttgccgg ctgcctccgg
     3001 cgacgcgcga ggcgagctgc gcgtgagcat cgaccgcaag cgcccggagc gcaccacggc
     3061 cgaaggaagc ctgcacatcg aggcgctcga cctgagctgg ctcgccggca ggcgcgcggt
     3121 ggtccagcgc gcagatctca gcaccgaagg cgatcgcctg cgcatcacgc aggctcggct
     3181 cgacgtggag gagcaggtat tcgatctgcg cggcgagatc cggcgcaccg accaggcccc
     3241 ggtgatcgaa gcgcgcgtcg agtctgcggg tgtgatgctc gatcggctgc tgccggctcc
     3301 agacccgagc gcgccgaaaa aaaagtcgtc cgcgctctgg ccgctgccgg tcgccgggcg
     3361 cgtcgaggtc gcggccggct tcatccagta caaggactac aggatcgagc cgttcgacgg
     3421 catcctgtcg ctcgagccga agcgcgcgcg cctagacgtg aaggaagcgc gcatgtgcgg
     3481 cgtctcgttc ccgatggagg tcgagatcca gcccgaggac gcctccgccg cggcgcacat
     3541 cacgatcaaa aaccagccgc tggagcgcac gcttcactgc ctgaccggcg gcaacgtgca
     3601 gatcacgggc aacgtcgacc tgaaggccga gctgcgcgtg caggggaggc ggccgcacct
     3661 cgtgcgtggc ctcaccggta cggtagaaac cgaggtgcgg cagggccggg tgaagaaatt
     3721 cgccctgctc ggcaacatcc tgtccttccg cggcatcgcc tcggtcagcg agatgaagga
     3781 ggagggattc ccttatcgcg cgatcacggc caaagggcac ttcgcgaacg gcgagttcct
     3841 ggtcgacgag agcttcttcg acagcgacgc ggtgcgcctc gccgcgcagg ggcgcgtgga
     3901 cctgctcggc gcgaactcgc agctcaccgt cctggtcggt ctgttgacgc acgtcgagcg
     3961 tatcgcaggc gcgattccga tcatcggcga cgtcttcggc ggctcgatga tcgcgctccc
     4021 catggaggtg cgcggcgaca tccgcgaccc ggtcatcgtg ccgctcggtc cgcgggcggt
     4081 caccaatcag ctcctgggaa tcttcgagcg cacgctcaag cttcccggca agctcgtgcc
     4141 cgcgccggaa gcgaagtagg gaaattcggg gacggagccc taactccagg cggcgattgc
     4201 gccgagcacc ttcagcccga acgccgtgag caccgcggtc gcggtcgctc ccatgctctc
     4261 gagatccatg gccgcccggc tacttcttct tcgcggccgg cttcttcggc ttttcctcca
     4321 cgaccagctc cagcgccagc gcctgggtgt aggcgatcgt gagcttgtcg ccgactttca
     4381 cgctttcgag attcttaacc tcgtccgaga ccggcagcgc cagcgtgttg cccttcggcc
     4441 cgcgcagcgc gacgtagcgg ttcttgtagt cgatggcgtc gaccacggcg ctgatcttgg
     4501 tggtgcgcac cttgatcccg cccggcgtgc cccccttcgg cgcgagctgc acctcctcct
     4561 cgaccgtcgc ggccggctcg ccgcccctgg tgagcgcgat caccgcggcc tcggcataga
     4621 cgaccttgac ccggtcgcca accttcacgc ggtcgaggtt ctgcgcctcg ggcggcacat
     4681 gcaggacgcc caggttgccg cgcggcccgc gcatggtgac ggtgcgcgcc ttcgggtcca
     4741 ccttggtgac ggtgacgacg gcctcgatcc ggtccgtgac gatcgcgccg cggggctggg
     4801 caaggaccag cgccgggaca agcagggcgg cagcgagtag ggtgcgtttc atggcgttct
     4861 cctttggacc gccattagag gcaccccgcg ggggcgcggc aattggacct tggtcccatt
     4921 gctctagtta cgtcggacct gccacaccag gtcggcgacc gaccctactt ccattttctt
     4981 catcacgcgc gcgcggtgca ccttcaccgt cttctcggcg atgccgagct cggcggcgat
     5041 ctgcttgttg aggtggccgg cgatcacgtg cgtcatgacc tcgcgctcgc gcggcgtgag
     5101 ccccgccacc cgcacgccga ccgaagagcg ctcggcacgc gcggcgcgcg ccgagcggtc
     5161 cttgtcgatc gccgtgcgca ccgcgtccag gaactggctc tggtcgaagg gcttggtgag
     5221 gaagtcgacc gccccggcct tcatcgcgcg cacgctgctc gggacgtcgc cgtagccgct
     5281 gatgaagatc accgggcagg gatcgcccga gcgggcgagc acctgctgga gctgcagtcc
     5341 gtccacgcca ggcatcgcga gatcgatgac cacgcaaccc ggggcatcgc ggtcgtgctg
     5401 ccgcaggaag tcctgcgaag aggggaaagt gcggactgca aagcccgccg cgttcagcag
     5461 gcggccgagc gacttcagga cggccgcatc gtcgtcgacg acgcggacgc aaggtacggt
     5521 gtcgtacaga gcagtctccc ttggcagcgc aggctacacc catttgatgg atatggggtt
     5581 atcttttcgt cgcgcagcgg gcctgtgaaa catcccgatc gttctggaat aagaatagga
     5641 atgccgaagc ggtaacaatc ttggaatcca gatggcgagc ggggcacttg ccccgtcgct
     5701 ccatccagta cacgacgttg cccgcgacga cgatgctacc ggagacgacc gcgctaccgg
     5761 cggtcacggc accgatcacg acaagcatcg aggcgcattc cttgccgctg catgaagcga
     5821 ggtagccgaa cggctcggcc tgcagcgtca tccggcgcgt ctcgatcctg cattcctcgt
     5881 cgtgcacggt cgtggtgcga gggtagaaga cgcagccttg aaccagtgcg gccgcggcga
     5941 ggccgaggaa tagtctcact tgtcatgccg ttatcatgga gaccgattcc catggcaatc
     6001 acccgtccga gggagggcct gctcgcgctt ctgtgtgcgc tggcccttgt tcccttgccc
     6061 gccgccgcgc agacctaccg ctcggacgag cactcgttcc gcgtggtcaa ggtcgtcgaa
     6121 ggattcgagc agccctggtc gctcgccttc ctgcccgatg ggcggatgct ggtcaccgag
     6181 aaggcaggcc gcctgcgcat cgtgtcgcag ggcaagctcg acccgaagcc gatcgaaggc
     6241 ttgccgcaag tgacggtgca cgggcagggc gggctgcagg acgtggtgct tcatcccgat
     6301 ttttcgaaga attccctggt gtacctcgca tacgcggcgc gcggcagcga cggcgtgggc
     6361 accgagctcg cgcgcggccg gctggtcggt catcgcctcg aaggcgttga agttcttttc
     6421 aagcaaactc ccaagggctc gcgcggccag catttcggcg ggcgcatcgt gttcgaccgt
     6481 gcgggctacg tgtatctcac cctcggcgac cgcggcgaga tgccgcgcgc gcagcgcccg
     6541 gacgaccacg ccggctcggt gatccgcctg cacgacgacg ggcgcctgcc ggcggacaat
     6601 ccgtttgccg gcaagccggg ctggaagccg gagaagttca ctctcggcaa ccgcaacatg
     6661 cagggcgcgg cgctgcatcc gcagaccggg atgctgtgga cccacgagca cggcccgcag
     6721 ggcggagacg aggtgaacgt gatccgcccc ggcgtgaatt acggctggcc ggtgatcacc
     6781 tatggcgtca actacggcat cggcacgaag atcggcgagg ggacccgcaa ggagggcatg
     6841 gcgcagccga tccactactg ggtgccgtcg atcgcgcctt cgggcatggc gttctacacc
     6901 ggcgacaagt tcgcgcgctg gaagggcgat ctcttcgtgg gcgcgctgcg cgaccagatg
     6961 ctggtgcgcc tgaagctcga cggcgagaag gtggtgagcg aagagcgcct gctgaaaggc
     7021 gtgctcggcc gcatccgcga cgtgcgcgcc ggtcccgacg ggttcatcta cgtgctcacc
     7081 gatgaaagca acggcgtgct cgcccgcctc gagcccgcga cttgagcttt acgtaacttt
     7141 acgcagcgga aaaacgccgc ttaccggtca ccgccacact ccttcgcact tcaccgaagg
     7201 agaacgcatg aatcgcttca gacgcatcac cctcgccacg atgctcggcg gcctcgccgc
     7261 cggcatcggc ttcaaggcct tcggctacag ccgccggcat ggatacggac cgatcgaccc
     7321 cgcggacctc gagaagcggc ttgaccggat gctcaagcac ttctacgtcg agatcgacgc
     7381 caccgaggag cagaagcaga agctcgagcc gatcgtgaag caggcggcga aggatctgat
     7441 gccgctgcgc gagcagctcc acgccgggcg gcgcgaagcg atcgagctgc tatcgcagga
     7501 ccgcgtggat ccggcggcgc tcgaagcgct gcgcgggcgc aagatccagc tcgcagacga
     7561 ggcgtcgcgg cgcctaacgc gcgcgatcgc cgaggctgcc gacgtgctga cgccggcaca
     7621 gcgcaagggg ctcgccaccc acttcgcccg ccgccacggg cgctggggac acgcctaggc
     7681 gcatgtggaa gcggccgtcg cgaaccgcgg gatcgtacgg ccgccctcgg tatttgcggg
     7741 agcgatcgcc gggggcctgt tgctgcacgg cgcctggcca gcggggctcg tgcctcatgc
     7801 cgtaagcggg ccgctcggga gtgccgccgt actggcggcc gtcgtgctgt tcctctcctc
     7861 tgtgcgcgcg ctgcggatcg ccggcacccc ggtgccgggc gatcgcccta cgactgcgat
     7921 cgtgcgcgcg gggccgtttc gctacagccg caatcccatc tacctgtctt tctctctgct
     7981 ccacgccggc gtcgcgtgct gggtcaacag cctctggctc atcgtcaccc tgatgccggc
     8041 ggcggcgctg atgtcgttcg tagtcattcc gcgagaggag cgctacctgg aggcccgctt
     8101 tccgtcggag tacttaccct acaaggccgc ggtgcgtcgc tggctatgag ggcggcgatc
     8161 atcctgctgc tctcggtcgc ctgcgccggt tgcggcagct ggaccgggtt cgggtttcgc
     8221 gccgacggcg ccgaaatggc gcgcctcagg gaggtgctgg cgctcgaagc ggggaaggtg
     8281 gtggcggacg tcggcgccgg gaaaggccag ctgacgctcg ccttcgcgcg cgaagtgggg
     8341 tcgaacgggc gggtgttttc taccgagatc gatgccgcgc ggctgaagca gcttcgcatt
     8401 gcggtcgtcg acgccaggct cgacaacgtc accgtcgtcg aagcgctcag ccacgagacc
     8461 ggattgccgc cgggctgctg cgacgcaatc gtgctgcgcc gcgtgtatca ccatttgagc
     8521 gacgctgcga gcatcaacgc cagcgtgctg cgggcgctgc gcccgggcgg cgtgctcgcg
     8581 gtgatcgact tcccgccgcc cttcttctgg agccgaggct cgctcggcgt cccggcgaag
     8641 acggtcgtca ccgaagtcgc cggcagcggc ctcgagccgc tccggctgat cgacgactgg
     8701 ccgggccgcg gcccgctcgg gagctattgc gcggtgttcc gcaagccgcg ggtatagtcg
     8761 aagcgcagtg gaacgcgtcc tcttgatcga agacgacgcc cggctcgccg cgatggtttc
     8821 ggagtatctg ggcggctcgg gttaccgggt caccaccgcg gcgagcggcg cgagcgggct
     8881 cgagcggctc gagaaagagc cgtacgacgc cctggtgctg gacctctcgc tgcccgacat
     8941 ggacgggctc gaggtctgcc gccggctgcg cgcgcgctgg gagctcccgg tgctgatgct
     9001 caccgcgcgc ggcgatgcga tggaccgcat cgtcgggctc gagatcggcg ccgacgacta
     9061 cctgcccaag ccgttcgagc cgcgcgagct cctggcgcgc ttgaaggcgg tattgaggcg
     9121 cgcaaatcgc caggggaaag acagcctgct gcgcttcggt cgcctggaaa tcgaccgcga
     9181 ttcgcgcgcc gtgcgcgtag ggggcaagga agcggcgctg acttccttcc agttcaccct
     9241 cctcgtgacg ctcgccgaga acgcggggcg cgtgctctcg cgcgacgcgc tgatgaacct
     9301 gatgaagggc gagaagctcg aggccttcga ccgctcgatc gacgtgcacg tctcgcgcat
     9361 ccgcgcggcg atcgaggacg acccgaagaa gccgcgccgc atcatcaccg tgcgcggcgc
     9421 gggctacgtg ttcgccaagc aacaaaactg atggcggaat tcggggtcag ggtggaaatt
     9481 acgcttgcgc gtacgcggca gcgtaattcc caccctgacc ccgaattcgg gtgctgaatg
     9541 cgcaggctct acctaagaat ctacttcgcg gtgctcgcga gcctggtggc tttcgcggtg
     9601 ggcgctggcg tgctctggcg cacgctcgcg gagccccgcg agggcaccga gctcggggtt
     9661 atcgcgcgca acgtgctcgc gccggccgag gcggctcccg ccgagcagca ggcagcgctc
     9721 gagcgcgtgg cgcgcggcgc gcgcatggac ctcgcactct tcagccgcga cgggatccgt
     9781 ctcgccgccg tgggcggcgc gctgaccgcg cccgagcgca ccggatggcg gcatagcggc
     9841 ggcgacccgt cgtgggcgac gcagctctca gacgggcgct ggctggtatc gcgcccgcat
     9901 caccgcaggg gaccggcttt cggcgttttc gccctcgcgc tggtcctcgt cgccgtcggc
     9961 gtcggcgcct atcccgtggc gcggcgcctc acccagcggc tcgagcggct gcagcgcggc
    10021 gtcgacgcgc tgggcgcggg cgagctgtcg gcgcgcgtca aggtcgaggg cagggacgag
    10081 gtcgcccggc tcgccgagag cttcaaccgc gccgcggccc gcatcgagga gctggtcggc
    10141 gcgcacaagt cgctgctcgc caacgcctcg cacgagctgc gcacgccgct cgcgcgcatt
    10201 cgcctggcgc tcgagctggg gaaggacaag cgcgagatcg agcgcgatat cgccgagctg
    10261 gagcagctca tcgacgaggt gctgatagcg agccggctcg acgccgtcac ttctctggag
    10321 aatacggaag aggtcgatct gctcgcgctg gcggcggagg aagcggcgcg ctacgacgtc
    10381 gaggcgcagg gcgagccggt ggtcatccgc ggcgaccctc ggctcctgcg ccgcatgatc
    10441 cgcaatcttt tggaaaacgc gcgtcgccac ggcgcgccgc cgatagagat ccatgtcaaa
    10501 ggtcgccaac tcagggtctg cgacgccggc gccggcgtgc ccgcagccga acgcgagcgc
    10561 atcttcgagc cgttctattc caagcgcgga accgggctcg gcctcgcgct ggtgcgccag
    10621 atcgcccgcc ggcacggcgg cgacgtgcgc tgcgagggga actgcttcgt ggttacgctc
    10681 tttattccgg cttgatgccg acttcgcgcg cggtgctgcc ccagatctcg atctgctgct
    10741 tgaggatggc gctgagctct tcgggcgtcg agctcttcgg ctcgaacgct agcttgccga
    10801 acgcgtcgcg cacctcggcg cgcttcagga ccaccgcgag ctcgcgcgcg acgcgatcca
    10861 cgacctcctt cggcattccc ttggggccga acacgccgcc ccacggcgtg agcgacatct
    10921 tgcccagtcc agcctcggcg gcggtgggaa cctcgggaag cagcgcgctg cggttcggaa
    10981 acatcgccgc gagggcgcgc agcttgcctt ccttgacttg cggcgcggcg gtgccgggcg
    11041 tcgagaacgc gatgtgcacg cggcccgcaa gcaggtcgat caccagcggc ccgtcgcctt
    11101 tgtaggggac gtgcacgatg tcgagcttct cgagctgcgc gagctgggcg ccgaagagct
    11161 gcgcggtgct gttgccggag ccgtagttga ccttgcccgg attggcgcgc gcgtaggcga
    11221 gcagctcggc gaccgatttc gccggcacgc tcggatggct gaacaggaaa aatccgaaat
    11281 acccgacgaa cgacaccggg gtgaaatccg tgaccgggtt gtacggcggg ttcttgcgcg
    11341 tggcgggtac ccagttgaac gcggtgttgg tcgcgtagaa gagcgtgtag ccgtcgggcg
    11401 cggccttcat caccgcatcg gccgcgatcg cgccgtcggc gccgggcttg gtgtcgatca
    11461 cgatcggctg gccgagcgcc tggccgagcg gtaccgagaa gatgcgcgcg ccaagctcgg
    11521 ccgcgccgcc gggcgggaac gggacgatca gacggatggg cttgtcgggg tactgggcga
    11581 aggccgtggt cacccagagc gaggtaagga cggcgagaag gcgattcatg gggaaattct
    11641 aaagctttcg cgcgagcgcg gtgagggcgc cgtgagggga ggcgagctcc tccagcaccg
    11701 tgaagtggtt gcgctggggc accgccaaca gggttgtatg actaagtttt tttgaatact
    11761 cctctgactg tcgacagagc tccggcagct cgtcggctcc gtaggcgatg aacaggggtt
    11821 tctccgcggc cttgaggaac atcggactgt tgcgcttctc ctcggcttca tcgaggcaca
    11881 gcttgtcgtt gatgtagcaa agccgcatcg gctcgaggtc atagattccg ctgatggcga
    11941 ggccgccttt caccgctgga tgatccattg ccatcgcggt gagatgcccg cccgccgacc
    12001 agccggagac aaaaagccgg gccttctccc cgccgagcag cggaatcgcg gtatgcagcc
    12061 aggtgagcgc ggcgcggatc tcggcgacga tgccgtcgag ccgcttctgc ggcgcgaggg
    12121 tgtagccgat gagggcgaca ttgatgcccc aggcgagcgg cccttcggcg acgaaggaaa
    12181 aaatctcctt ggcgcggttc tgccagtagc cgccgtggat gaacgcgagc accgggccgt
    12241 cgcgccgcgc ggcgaaatag tcgatgcggt tgcgctcgtc cgggccgtag cgcaggtcca
    12301 ttccctcggg gtgcgcggcg cgcagccgcg cgctgcgcgc ttcccagtcg gcgacgatct
    12361 tcgggctgtc ggcgactgcg gcgccgttgt tgtaggcggc atcgagcgcc gcgcggtcca
    12421 ttccgcggta catcagacct tgaacgactt gagggtctcg cgcgcgaaca gctcgtcggg
    12481 cgcgaagcgc cgcttggaca gcccctgctc gtggtgatag cgcaggaagg tctcgaggac
    12541 cttgcggttg ggctcaaggc catacggcca ccagtcttcg cccatctcgc gccgcgcctc
    12601 ctccagctcg gccacgagcc acggcaccat cgccggcaac gccgcggtct ggtcgtagag
    12661 tcgataggct ttcgccttgg cctccgcgaa tgccttgtag agcgattgcg ccacccacgg
    12721 gttcttcgcg tacacgtcgc gccggatcac gacggtgtgc atcgtgggaa agatcttcgt
    12781 gcgccggtag tagtctttct ccagttccac gtagttcggg aacagccgct tcaccttgtc
    12841 cggctccttg tggaaggtgg aaggcgagcg cgcggtgacg agggcatcca gctcgccgtc
    12901 ggcaagcatg cgggacaggg tcttgtcccc aggaatagct ttcaagcgga tgttgggagg
    12961 taaattcagc ttcagcttct cgtcgcgccc gggctcctcc tcgccgccgg agtagtgctc
    13021 gacgtcggtc accttcacgc cgtgatcgtc cgagaggatg ccgcggatcc acaccggcgc
    13081 ggtcatctgg tactcgggca cgccgatgcg ccggcccttg agctcttcgg gctttcggat
    13141 gccgctcttc gccgacacga agatgcacga gtggcggaag tagcgcgacg ggaacaccgg
    13201 aatcgccacg aacggcgggt tctcggcctg cagggatgcc acgtaggagg agagcgacat
    13261 ctccgagcag tcgaactccc ggtagcgcag catgcggaag aacgtttcct ccaccggctg
    13321 gttcaggtag acgagctcga tgccgtcggg cgccaccgtg ccgtccatca gggcgcgcgt
    13381 gcggtcgtag tcccagcagg cgagcgtcag gcgtagcgcc ggcatcggtg aaggggcttc
    13441 ttaccggttt cgcagcggaa tgtcgacgcc ttcgcgcgcc gaacgcaaga tcgcaagcga
    13501 cacctccagg ttccccatcg cccattcgcc gctgtgcagc ggtgcgcgtt tctcgacgac
    13561 ggccgcgtag agctcgtcga tcacttcctt gcgcggcact tcgggcagcg gcagccgatc
    13621 gaagcgtctc tcgttgtcgc cgtggatcat gaccccggtg ggcaggggct gcaggtcccc
    13681 cttctcgcag gagacgatca ccacgccgaa gtgctggtgc gcgaccggcg ggatttcctt
    13741 gaactccggc ccgccgtagt tgcgcgcgtt cttgacggcg agctcgtcgc cgctggcgag
    13801 ggcgcgccgc gccgtgccgt actggcgagg atccctttcc tgccccattt ccccgatcca
    13861 gcccatgaac tcgtcgctgt cgaaatgcgc gtagccgctg tagaccaggc tcgcaaaggc
    13921 gccgttgtcg aagcccagca ggcaggtata ggcgccctcg gtctgacgcg acgggtccca
    13981 cttgccggtg taggcgcgca cgctgcggac gttgccgccg gcgagcaggc gcacgatgtc
    14041 gacctggtgc gccgcctggt tgaacaccac gccgccgccg aactcggtgt cgagctcgtc
    14101 ggggcgccgc ggccggtaga ggaagtcggt gtagtcgagc gcggtgacca tgcgcaccgc
    14161 gccgtaggcg ccgctttgaa tcaggtcccg cgttcgcagg atcggggcgt cgaagctgtg
    14221 gctgtggccg acgatcagcg cgaccccggc cttgcgcgcg acgtcgatca tcgcgcggca
    14281 gtcctgcagg aagagggcca tcggcttctc gaccaggatg tgcttcccat gcgacgccgc
    14341 gaggcgcgtg tggggcgcgt ggaactgatg gggcgtcgcg atgtagacca catcgacccc
    14401 gggatcggcg cacaggtctt cggtcgaagt gaaaacccgg ccgccgtggt cggccttgaa
    14461 cttggcgagc gcctcccggc gcgggtcggc ggccgcgacc agctcgaccc gcggatcgcg
    14521 gaaagcgcct gccatgaccg caaatgcgcg gcccaggccg gctacgccga ttcttagctt
    14581 tttcatgcta tctatatgcc ctatgttaac caaggagcag aaccagctcc tgtgccgcgt
    14641 ggaaggcgac gccgtgatgg ggcaaatgtc acggcgtcac tggcttccgg tttgcatggc
    14701 ggaagaggtc ggccaagccg acggcgcgcc ggtgcgcgcg cggctcctcg gcgaagacct
    14761 tgtcgttttc agggacacaa cgggaagact gggtgtcctc ggcgagcact gcctgcaccg
    14821 gcgtgcgtcg ctcgttttcg ggcgcaacga ggaatgcggt ctgcgctgcc tctaccacgg
    14881 ctggaaattc gacgtcgagg gcaacgtcgt cgacatgccc tccgaggcgc ccggcgcggc
    14941 tgcgcgcctg ggcaagaaga ccaaggctta tcctgcgcgc gagggtggcg gcttcgtctg
    15001 gatctggatg ggcccgcgtg aagaaatgcg cgagttcgag ccgcccgctt gggcgccgaa
    15061 gcccggcatt cgctacgcga tcgtcaagat gcacgccgcg tgcaactggg cgcaggtgct
    15121 cgaaggctcg atcgattcgg cgcacagctc gagccttcac tcaaccaaca tgccctccgc
    15181 cgaggtggaa ggatcgaccg cgaccgactc ggcctggctg cgcccgtcga ccgacaaggc
    15241 gccgaaattg cagttcgagg cgacgagcta cgggttccgc tacgccgcca tccgcaagcc
    15301 gatccgcaat ccggagacgc accagtacgt tcgcaccacg ctctatatcg cgccgttcac
    15361 ctgcctgatc ccgccgaacg accagtacaa cctggcgcag atgctcatcc cgatcgacga
    15421 cgtgaacacc ctgttctact ggatcgcgtg gcatccggac ccgaagaagg gcatcacgca
    15481 gcaggcgtgg cgccggttct gcggggccga agtcggcgtc gacctggatg aaaatttccg
    15541 gaaaaagaga aacctggaca accgctatct gcaggaccgc gacgccatga agcgcggcga
    15601 ctggaccggc atccacggca tcccgacgca ggacatggcg atgtgggaat cgatggggcc
    15661 gatctcggac cgcagccagg accatcccgg cgcgagcgac ctcgcggtgg cgcaattccg
    15721 ccgcctggtg atcgccgcgg cgaagaaatt ccaggaaggc gggcccgcga tcggcgccgc
    15781 agagccgcgg gtacggcagg tcacgctcgc ctcgttcgaa ggcgtggtgc cgaagagcgt
    15841 cgactggcgc acgctgggcg cgcctcagga agagccggtc ggcgcgaaat aggttgcacg
    15901 accgacatta ctccaggttg taatgcacct ggagggtcac gaagttgatc ccggggttcg
    15961 ggctgtggaa gccgccgttc gaaatgtgct gcacgcgcac gccgaagtcg tacttgccgg
    16021 aatggatccc ggcgccgagg tggtcggcga actgcagcgc ggtgctgaac gagcgttgcg
    16081 cgctgatccg cgcctgcacg aggtgcacgc cgaccgcccc ttcgaagtag agcgtgcccc
    16141 tctcgatgcg gaacaccggc gtaaagccga cgtcggcggt gctttcctcg cggttgtccc
    16201 aaaggccggc cgatagctcc cagtagccgg cgagccgcca ttcggtcgcg ggcgggcgct
    16261 gcgcccggtg ccagcggtcg gtgaggatga agcgcagcag gctggtgcgg tcgtcgccgc
    16321 ggcccacttc cacggcgacg ccatcgatgc agt
//
LOCUS       DATBSZ010000071        27078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_78180, whole genome shotgun sequence.
ACCESSION   DATBSZ010000071 DATBSZ010000000
VERSION     DATBSZ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 27078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27078
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78180"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="VIG70_04585"
     CDS             complement(<1..148)
                     /locus_tag="VIG70_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY8249861.1"
                     /translation="MTSPVDRIRGYHAELREFRRDLHAHPELAFEESRTSALVVESLK
                     KWGIE"
     gene            complement(145..1116)
                     /locus_tag="VIG70_04590"
     CDS             complement(145..1116)
                     /locus_tag="VIG70_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8249862.1"
                     /translation="MAAFILRRIAQAVLVMLTVGLIAFSLFRFVGDPVTFMLGQDATP
                     EQRIEVTRALGLDQPFYVQYVKFVGNALQGEFGLSLRQVQPVSRLIVERLPATLELSI
                     VAALFALLAGIPMGVYTALRPRSFFSQVLLAVSLAGVSLPTFLIGILLILVFSVQLGW
                     MPSFGRGDVVQIGWWSTGLLTASGLRALVLPAITLGLFQMTLIQRLVRSEMLEVLRTD
                     YIRFARARGLTDRAVHFGHALKNTLVPVITITGLQLGAIIAFAIITETVFQWPGMGLL
                     FIQAVSFADVPVMAAYLCLIALFFVLINLAVDLLYYAVDPRLRLQTP"
     gene            1091..2125
                     /gene="mutY"
                     /locus_tag="VIG70_04595"
     CDS             1091..2125
                     /gene="mutY"
                     /locus_tag="VIG70_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY8249863.1"
                     /translation="MRRRMNAAILRECYPMSFAEKLIGWQRRHGRHGLPWQGTRDPYR
                     IWLSEIMLQQTQVGTVVPYYMRFIKRYPTVEALAAASEDEVLQLWSGLGYYARGRNLR
                     LAAQSVVSRGAFPQTAAQIAELPGVGRSTAAAIAVFAFGERAAILDGNVKRVLARRYG
                     IADKDELWLLAERLLPRKNVETYTQALMDLGATVCTRKPDCGACPVRAGCVARNTGRI
                     AELPAPRLRKALPLKQATWFVYRDAGRVLLERRPSSGIWGGLWCFPERPLVGSSDPKK
                     LATIDHGFTHFRLRIHPLLHEVRKGARIDFPGLWVDLHDALRAAIPTPVKNLLQELQR
                     NATRGSRSRR"
     gene            complement(2384..2953)
                     /locus_tag="VIG70_04600"
     CDS             complement(2384..2953)
                     /locus_tag="VIG70_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY8249864.1"
                     /translation="MEDLPLFPLHTVLFPGGRLPLRIFEARYMDMAKACLKDGSPFGV
                     CLIREGKEVGAPALPAAVGCLARIAEWDMPQLGLLQVIARAERRFRILERRVQPDGLA
                     RASVELLAEEADGVPPEKLSRCVRLLERVIEQHPGLIEQPHRLDSCSWVASRLAELLP
                     LSLETKQALLELDDAKVRLERVHALVRPD"
     gene            complement(2950..3804)
                     /gene="rapZ"
                     /locus_tag="VIG70_04605"
     CDS             complement(2950..3804)
                     /gene="rapZ"
                     /locus_tag="VIG70_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY8249865.1"
                     /translation="MPQNLVVVSGLSGSGKSIALKVLEDAGYYCVDNLPATLLLEVVD
                     FLTLEAGHERVAVSVDARSASLPALPENIERLKNRGFECRVLYLEASTPTLLRRFSET
                     RRRHPLSGSMSLPEAIERERALLEGVAPLGQRIDTSDLQPRVLQNWIRDLLGLGAGAL
                     TLLFESFSYRQGIPLDADWVLDARMIANPHYDAQLRPLTGRDAPVIDYLARDSLAQQW
                     LEDVRALLARWLPEIVRENRSHVTVAIGCTGGRHRSVYLAEKLAAAFRADWKVLVRHR
                     GLTEEPPP"
     gene            complement(3789..4178)
                     /locus_tag="VIG70_04610"
     CDS             complement(3789..4178)
                     /locus_tag="VIG70_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEY8249866.1"
                     /translation="MVLRAILIGMALSFSAQAQEKKPTAQQERMAECNKRASAKGMKG
                     DERKDFMSSCLKDKAPSAAQQAQQERMADCNKQASAKGMKGDARKDFMSGCLKDSPQD
                     RMTKCNKEAAGKKGDERKKFMSECLKT"
     gene            complement(4178..5104)
                     /gene="hprK"
                     /locus_tag="VIG70_04615"
     CDS             complement(4178..5104)
                     /gene="hprK"
                     /locus_tag="VIG70_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEY8249867.1"
                     /translation="MLQVNVARLHDDNRESLSLAWVAGREGGTAAVRREAAQAASLIG
                     HLNLTHPNSIQVIGAYEAGIVGGFVDKLLESSPAAVIVADGVAPPLALVESATRTRTP
                     LFTSPLPAPRVIERLARYLAKALAETVELHGVFMDVLGLGVLITGESGVGKSELGLEL
                     ISRGHGLVADDVVEVSRFGAGGLEGRCPALLKDFIEVRGLGVLNIRTIFGETAVRRKM
                     KLRLVAHLARPAPGTAGAARDPTERLPLSELSEEILGVTIRKVIIPVAAGRNLAVLVE
                     AAVRNEILKLRGIDSMAEFLARQTQEIQKGEE"
     gene            complement(5073..5558)
                     /gene="ptsN"
                     /locus_tag="VIG70_04620"
     CDS             complement(5073..5558)
                     /gene="ptsN"
                     /locus_tag="VIG70_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEY8249868.1"
                     /translation="MTLVSKILSVSHVSVDLQASSKKRLFEQAGLLFENLDGIGRSLV
                     YDSLFARERLGSTGLGQGVAIPHGRIKGLKEALGAFVRLAQPVPFDAPDGNPVSLVFV
                     LLVPEKATEKHLQILSELAQMFSDKALREAMTTAPDAAALHQLIAAWQPDAAGQRRAA
                     A"
     gene            complement(5555..5848)
                     /gene="raiA"
                     /locus_tag="VIG70_04625"
     CDS             complement(5555..5848)
                     /gene="raiA"
                     /locus_tag="VIG70_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY8249869.1"
                     /translation="MNLSVSGHHLEVTPALRSYVEAKIGRVTRHFDHVIDAHVILTSS
                     KLTQKAEVTLHVSGKDLHCESEEGDLYAAIDLLADKLDRQVLKYKDKLYAKRA"
     gene            complement(5848..7260)
                     /locus_tag="VIG70_04630"
     CDS             complement(5848..7260)
                     /locus_tag="VIG70_04630"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY8249870.1"
                     /translation="MKPSLQFRLSQHLTLTPQLQQSIRLLQLSTIELNQEIDRILMEN
                     PALEREDHEEGVEVQAKGPAAETPAPASTSEGPGSEAPDWPSEVASNWRGPEDDDEGD
                     RTHATPDTPTLRDHLRAQLSLTNLGPRDRAFVELLIDALDEDGYLTQSLEEIAAMLTA
                     HLPGDPEAGLEDLSIALCHLQHFEPAGVGARSPAECLSLQIKNLPDDLVRGLALEIVD
                     KHLDLLAARDYTRLKSATGADDDALRAAQRLIQALNPRPGAAFAKVEARYVIPDVIVK
                     KLRNVWRASLNADAMPRLRINRLYAELAAAARSGAGISSQLQEARWLIKNVQQRFDTI
                     LRVSQAIVDRQRHFLEHGEVAMRPLVLREIAGTLGLHESTISRVTTQKFMATPRGTFE
                     LKYFFGSHVATEAGGAASSTAIRALIKQLVAAEDAKAPLSDARISQILGEQGIVVARR
                     TIAKYRESLQIPPVNQRKAL"
     gene            complement(7283..8005)
                     /gene="lptB"
                     /locus_tag="VIG70_04635"
     CDS             complement(7283..8005)
                     /gene="lptB"
                     /locus_tag="VIG70_04635"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY8249871.1"
                     /translation="MSELRSQRLRKRYKNRTVVQDVSLEVSSGEVVGLLGPNGAGKTT
                     CFYMIVGLIPVDAGSIHLDSRELTHLPIHRRARLGISYLPQENSVFRRLTVEENVQAI
                     LELQGLGAEEIEPRLAELLKDLNIAHLRKHAAISLSGGERRRLEIARALGTRPAFILL
                     DEPFAGVDPIAVLDIQRIIRFLKERGIGVLITDHNVRETLGICDRAYIINEGAVLASG
                     HPEEIVYNDSVRRVYLGENFRM"
     gene            complement(8013..8525)
                     /gene="lptA"
                     /locus_tag="VIG70_04640"
     CDS             complement(8013..8525)
                     /gene="lptA"
                     /locus_tag="VIG70_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEY8249872.1"
                     /translation="MKRAWIAAALLACSSVLAEKADRDQPTQVEANKMSADDARRLNV
                     FEGNVVVTKGTIRLTADRLVVRQDAEGFQFATATGKPARFRQRQDPKPGEKEGIWVEG
                     EALRIELDDRNQKIELFDNARVNRGGDQVAGNYIFVDQRSDFYQVTAGKGDRVKAIIQ
                     PKAPSAEAGK"
     gene            complement(8522..9097)
                     /gene="lptC"
                     /locus_tag="VIG70_04645"
     CDS             complement(8522..9097)
                     /gene="lptC"
                     /locus_tag="VIG70_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY8249873.1"
                     /translation="MRFSTTRLFPLGLMLALALLTFYLERTVREEDAPPALRRHDPDY
                     IVAKFTTTTYNRDGDAETVMSAERMVHYPDDDTTEIFAPRVVQTKPNQPRYTVRAERG
                     QLSRDGDEIFLYGDVVLVRDASAEGPEARMTTEFLHVLRDRALVRTDREVKIVEGRRS
                     LTGRGMEYNNASRELLLRSDVVARFTPDPAE"
     gene            complement(9094..9579)
                     /locus_tag="VIG70_04650"
     CDS             complement(9094..9579)
                     /locus_tag="VIG70_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit delta"
                     /protein_id="HEY8249874.1"
                     /translation="MIFDVDGVLTDGTLLYGPAGEELKAFSAHDGHGLKMLAASGVAC
                     ALLSGRSSAAVALRAAELGIKDVLQGIEDKLAAFQKITREKDLTVESIGYMGDELVDL
                     PVLTRCGFACAPREAPEAVRARVHYVAAAPAGRGAAREVCELVMRAQRTLEKALQSYL
                     G"
     gene            complement(9627..10646)
                     /locus_tag="VIG70_04655"
     CDS             complement(9627..10646)
                     /locus_tag="VIG70_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY8249875.1"
                     /translation="MIKAASRVRKTNHGSSALELGRRVLEIEADAVRALITRLDQRFV
                     DAVELIAARKGRVVVSGMGKSGHIARKIASTLASTGTPAYFVHPAEASHGDLGMVEAG
                     DVFIAISYSGASEELVQILPQVKRRGAKLIALTGKPDSALAREADVHLDAGVAQEACP
                     HNLSPTASTTAALALGDALAVALLDRRGFSAADFARTHPRGGLPGQALARQALLHVED
                     VMRTGDQIPKVASGTPLRQAVIEMSRGRIGMTAVMDPAGLVVGVFTDGDLRRTIEKST
                     ELNQVLVDDVMTKSPRTVRPKTLAAEAVHVMEEHKVSQVLVVDERGALVGALNMHDLF
                     RARVI"
     gene            10665..12644
                     /locus_tag="VIG70_04660"
     CDS             10665..12644
                     /locus_tag="VIG70_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEY8249876.1"
                     /translation="MDPSDIAVNPGVQSVLVLLASSVLAVAACRSLRLPPMIGYLVTG
                     LALGPHALGVVSEREEINRLAEFGVVFLMFSIGLEFSLTKLKSMRRLVFGLGLAQVGA
                     TLALAVAAAAALGAPWQMGLALGGIMAMSSTAIVSKLLAERGELDSAHGREVIAVLLF
                     QDLAVVPLLVLIPVLGQPADAIGLAVAAALAKAAAALFIVIVGGPRPMRAWLRTVARR
                     RTNELFVLNVLLVILVAAFITAAAGLSLVLGAFLAGMLISETEYRFQVEQDIKPFRDV
                     LLGLFFITVGMMLDLRLVAAQFWLVVLFLVLLMIAKFFLIAGLSRVFGAAPGTALRVA
                     LALAQGGEFGFVLLPLAGAAGIVPDALLQPLLAAMIVSMLCTPFVIAASDRIVLRLSR
                     TEWLQRSLALHRVAVQSLEAERHVVIVGYGRNGQRLARLLDAEGVRYVALDLDPERVR
                     EAAAAGDTVVFADGLRREALVAAGIMRAAAAVLTFADAATAVRMLAHIHDLNPSLPVI
                     VRARDEADIAPLTAAGASEVVPEAFESGLMLASHALVWVGVPLSRVMRRVSQVRGERY
                     GLLRGLFHGREREDGEGDAGAQLHSVTLEPGARAIGRTLEELDLGVQVRAVRRRGVKA
                     RLDAASARELQEGDVVVLLGPPDALAAAEERLAAR"
     gene            complement(12529..14241)
                     /locus_tag="VIG70_04665"
     CDS             complement(12529..14241)
                     /locus_tag="VIG70_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HEY8249877.1"
                     /translation="MNATSRPRAGFFFADPARISLALAGLLAAIPFLFPEHRPPLRSF
                     YDEWIGAGLATLAIAAFLPSSRSVKDAVPDLSVWLLLFAGWLGLQAALRPPAYAQLPV
                     AGVAYVLLAAQLAWLGHALAARFGAERVVDVLAWALLAGALANAVIGILQFYGVPRFL
                     SGFIGTTSGPRIVGHVGQPNLFAEYVALGQAALLYLVARGLLRGNWWWSAAALLVLSS
                     AYTQSRSAILFSLWICALGFALRHRGPPWRRLALHAGILALATLVAMALIRASALDRL
                     ADGTGEPRLALWGLAWRLFAGSPWLGVGWGEFAGAAFAAGLPPLLGAYEVVWTSAHNI
                     VLQLLAETGLVGAAVIVVAALRWWARAWTSLRGSPALPQWWIVAAVGTISLHALVEEP
                     LWYAHVLCLAALIAGIASATTAKLPAVPVRLCLLAVNLAALAVLAWSLFDYQRFDRAY
                     VVASGRTLAPAEEVSAALSELRAAARGPLGAQVAPWLYQSLPLAEDAAAAVEMGERAL
                     RRWPSARVIARHAAARELLERPRSPSAPPSAPPRPQARREDRAAPRRRLPAARAPRRR
                     RASP"
     gene            complement(14255..14905)
                     /locus_tag="VIG70_04670"
     CDS             complement(14255..14905)
                     /locus_tag="VIG70_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8249878.1"
                     /translation="MKAAQAKQQGFTLVEIAIVLVIIGLLLGGILKGQEMITQAKIKN
                     VVSDFSGVSAAYYGYQDRYRAIPGDDINAATRWTVAPAAVAGDGNAVVAGKYNSQTDA
                     NESRKWWDHLRRAGFVSGSGYSQPINAVAGIIGVQTGDAAGGPAMGGIPGLIVCSTNL
                     PDKIAIAVDTQMDDGAPDKGTVRGDAQTGSNPDLDDALPSGYQETGTNIYVVCRAM"
     gene            complement(15048..15575)
                     /locus_tag="VIG70_04675"
     CDS             complement(15048..15575)
                     /locus_tag="VIG70_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249879.1"
                     /translation="MTMIYLQSYDLGGANTTPYQKLDYRRLVDWLRSILELDPRSQYP
                     LFAAARVYTETPDPVRLRIALEFVHEEFLRDPNRRWPWLAHAALLAKHRLKDLPLARR
                     YAHDLHRQTTARDVPLWAKQMEIFILEDMNELEAAKIMIAGLLETGAVSDPAELRFLK
                     QRLDELEARAAKENR"
     gene            complement(15767..16528)
                     /locus_tag="VIG70_04680"
     CDS             complement(15767..16528)
                     /locus_tag="VIG70_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8249880.1"
                     /translation="MPAATLARSVLLEARRGGLPWLAAAAVALGLVLAAFVSQVALTE
                     SLALQAALIAAVLRACAVFLVAAQVTASTLREVHDKGLELMLSLPLSRTTHYLGRLAG
                     FVACGAALAVVFALPLLLWAQPGAVTLWGLSLACETALVAAAALFFAMTLGQFVPAIA
                     ATGGLYLLGRSIGTIQAIASGPLAEASAASRIAEYAVDAVALVLPRLDALTRTAWLLY
                     DPPPAGVYAAALASAALYTVVLAAAGLFDFYRRNV"
     gene            16561..18270
                     /locus_tag="VIG70_04685"
     CDS             16561..18270
                     /locus_tag="VIG70_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8249881.1"
                     /translation="MQPAVLTRLANRTWTFSQRYWRIVVVAMLALLHVAVFRGVTDPW
                     ARALMLAHLGLLLLWQPFLRAEQRVSATQGLMLALVATGVMLWLDWWFLAFWVVVLAG
                     LVGGKAYQQHARWQRRCYLVVLAYLLALLAIAILPEIAPRREIDAEIRDYAEYLLPLA
                     FVLIALFPAEPDAAEAAHLIDFFYSLFLMLLLVVVILGSFTFMSLRRTPYLEALTYTV
                     FITAGALLLVALAWNPRAGGGLEVFFARYLFSIGLPVERWLHFLAELSLVEARPERFL
                     EEAVAALVRLPSVAGVRWRAGEASGEQGSRSAQAVDYAASELELTIYSRYRLGAALRW
                     HLHLLGQLLGEFHVAKLREEKLRQASYLQAVHETGARMTHDIKNLLQSLSVLTSVAAR
                     DDSGSSQLHGLVRRQLPLIERRLADTLEKLQRPQATGETYVAARGWWEALARQYRGEG
                     VEFDAIDPPPGTRVPRSLFESVADNLIRNALAKRADDEGLRVRVSLECGERVALRVCD
                     SGTAVPPEISASLLRAPVSSRSGLGIGLYQAARQAESNGYRLELETNRDGEVCFVLTG
                     LAG"
     gene            complement(18267..18947)
                     /locus_tag="VIG70_04690"
     CDS             complement(18267..18947)
                     /locus_tag="VIG70_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8249882.1"
                     /translation="MRRARGFTLVELAVALAVIALLLGVLAVPLNTQIDQHRINETQK
                     QANLIVEAILGFAVTWGRLPCPAAATTANTMAGAGSEARTGTACTQPEGVVPWAALGV
                     AETDAWGRRFTYRVTAMLADDPTGGLQASFALTDTGNLTVRASAAGVDIASNIAAMVV
                     SHGKNGFGAFQTNGAQVAGAAGDELENADNDGIFVHKLHEPAFDDVLAWVSPNVLKSR
                     MVAAGRLP"
     gene            complement(18944..20437)
                     /locus_tag="VIG70_04695"
     CDS             complement(18944..20437)
                     /locus_tag="VIG70_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249883.1"
                     /translation="MALVLVAVLLLGGAAFAFSSFNLTTVRVDRDRSTNEALARAKDA
                     LIAYAAADTARPGELPCPDVNDDGKLVIGQDIIGSACASLIGRLPWVTLGLPDLRDDA
                     GERLWYTLSNDFHANGPVPLNSDTAYRAANVSLTMTGTSPATNLVAIVFSPGAVLTRT
                     DGRVQTRGCGAACDPRDFLDIASGEDNADANRTFVSAERSGTFNDRLMPVFSDDIMRL
                     VEKRAARELAQHLRNHYNAWQNSTVSGVKGFYPFAVPWNDPSSTPAQLGTDGTTAGLL
                     PLSTAAPVWSNATPVVCTGNGTPILECSSLVVCLFGICVPDIASARIDNVATRFIDPP
                     QAANFQLLGLSLGGSASWTLNKAARRLEFDYSGILVAGWLDFRVTAPPISSWVTGWLG
                     DNNWHQNAYYAFSPGYAIDGVDACGGAAPACFTVTNTGAANDDNHAVVVMTGRALPSA
                     AQIARPVTPLPANFNQFFEATNAGVGTVFESNARTASFNDTPVGVKP"
     gene            complement(20476..20889)
                     /locus_tag="VIG70_04700"
     CDS             complement(20476..20889)
                     /locus_tag="VIG70_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249884.1"
                     /translation="MTRLFAALLLMTAQTCFAQELGRLFFTPEERSALDARRRARVPD
                     KPAAAVVASPTTKLDGYVTRSRGPSTVWVNGEPLPEGSSDAPRIGRSQEGRVSVPVGD
                     GGRRAGLKPGEVIDRGTGEVRDVIGDGEIRIRRAE"
     gene            complement(20886..21494)
                     /locus_tag="VIG70_04705"
     CDS             complement(20886..21494)
                     /locus_tag="VIG70_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249885.1"
                     /translation="MNLTREELKKIALPLLAAAALLGAGAALIWLAGQSLTANQRQLA
                     AVQAERRQSTERLARIAEEEREVKEKLDVYKQLKGLNILGEERRLEWADAITRIRTQR
                     ELLDLSYRIDRQRMLKSVPGKPDNVEFLASTMRVQLALLHEEDLLRFLSDLRASGNAY
                     YSVKSCQLARTGQAATGVTITPRLRASCEIDLITVVDRGAKR"
     gene            complement(21491..23041)
                     /locus_tag="VIG70_04710"
     CDS             complement(21491..23041)
                     /locus_tag="VIG70_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249886.1"
                     /translation="MAARYLLYFTAEDHYLYCSRHGRLELEAKFAGDDLGVTAFREHL
                     RGHKGALFAVVADLAGEDFHEDQVPLLRGGDREAIVQRRLAQRYRDTRLAAALSLGTI
                     AGTQRRNERLLLASFTNTQQFAPWLDALEEAGARLAGVYSVPLLAPALAARLGARGPR
                     ALVITANRAGLRQCFVEDGKLRFARLERAVDMVPQALAAFARSETLRLVQYLATLRAL
                     PREGPPIPALVVAPRGERAVFEQALVSDARVVFRTIDGAQALAAVRLRQVPEGAAAEA
                     LYLHLAARQPPREQFASTGDRRRYFVWRLQRAVVAAGALGFAACAVYAGTRWFESYDL
                     ASRAAAQSREASLAAQRYERITASFPVTETTTENLRVTVLEFRRIAEASASPEPAFVH
                     VSQVLARFPQVEIDSVNWSVGRPGKPAAQAAKPAAQPVKPEAQSEQAVLVEISGRVRA
                     TQRNDYRGITAQVQSLAGALAGSGYTLVRTQLPFDVTSEGTLTGDIGGSDSGEAPRFT
                     IVLARRLP"
     gene            complement(23047..24150)
                     /locus_tag="VIG70_04715"
     CDS             complement(23047..24150)
                     /locus_tag="VIG70_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8249887.1"
                     /translation="MELDLVAGAPASDMSRLLGGGKVKRQDLINFCFHLEQLASAGVP
                     LVEGLADLRDSVENLRFREVVSGLIEAIQGGQSLSQALADYPSVFSKVFISLVRSGEQ
                     TGKLAEVLKSLTESLKWEDELAAQTKKLAMYPAFVGGIVLVVTFFLMIYLVPQMTAFI
                     RNMGQEIPLQTRILMVVSGLFVDYWWAILAAPFAAWGALKLAVRSNPRVAYAVDHYKI
                     SMPLLGPILRKIILSRFASSFAMMYSSGITVLDSIRSCEEIVGNLTLERALRRAGQQI
                     AEGKNLTAAFQDLAVFPPLVIRMLRIGEATGRLDTALLNVSYFYNREVREAISRTQAM
                     IEPALTVLLGAILGWVMLAVLGPVYDAISKMKF"
     gene            complement(24267..25976)
                     /locus_tag="VIG70_04720"
     CDS             complement(24267..25976)
                     /locus_tag="VIG70_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY8249888.1"
                     /translation="MNQPAPPQAKRHLGQILIDQGILTEDQLRIALLEQTKQHIPVGK
                     LLVQLGFVSEATLRDALSEKLGLQSVDLARIIVDPAALKHLPREMARRNRMFPVALDR
                     QQRKFIVALADTNNIVAIDQLRAHLKGEYEIELRLAGDSEIERAIEHYYGHEFGIDGI
                     LKEIETGEVDYATLSEGDEYSQPVVRLISALLADAVERGGSDIHFEPEQNFLRIRYRI
                     DGVLRQIRSLHKTYWPAMAVRLKVMAKMNIAETRAPQDGRISATMSGRVVDFRVASMP
                     TTHGENLVLRILDRQKGIVPLEKLGLEETQLELLKRMIARPEGIILVTGPTGSGKTTT
                     LYSILNHINSDGVNVMTLEDPVEYPMTLIRQTSVSEASKLDFANGIRAMMRQDPDVIL
                     VGEVRDHETAEMAFRAAMTGHQVYTTLHTNSAVGAFPRLLDIGIVPDILAGNIIGILA
                     QRLVRRLCRACRQSYDPDVKERRLLGFSPTEPAALFRAVGCEKCEYQGYSGRQSILEL
                     LKIDAGIDELVARRATAREILTAARASGFKTLADDGVRLARSGATSLEEVMRVVDLTD
                     RMA"
     gene            complement(26050..>27078)
                     /locus_tag="VIG70_04725"
     CDS             complement(26050..>27078)
                     /locus_tag="VIG70_04725"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8249889.1"
                     /translation="KAEPQASERAAARKVFEAKFKESNPRLPFFITMGALGAFAVGTV
                     VYFWIQLRPPAPLVNTNPQRPTGEQVAAAPAAAPRPSAPAASAAQSAIPGLPAPAPAP
                     AVPAIPAKPAPPVAETPLLKPQPRVLAPAPAPRAPAPAPAESEISTRRPVPQVHPKVG
                     SAYAAYQAGDLPTARSDYQQALREEPANRDALLGLAALDVRSGRLEAAEGAYLRLLQA
                     DPRDAHAQAALLALRAGRVDPVAAESRVKSMLADNPGAHVLNFTLGNQLAQQGRWAEA
                     QQEYFKAFAAEPDNPDFAYNLAVSLDHLRQPKVALEYYRRALALAKTRGASFDTAAAQ
                     ARVDQLGH"
BASE COUNT         4020 a         9153 c         9611 g         4294 t
ORIGIN      
        1 tctcgatgcc ccacttcttc agcgactcca ccaccagcgc cgaggtgcgg ctctcctcga
       61 aggcgagctc ggggtgggcg tgcaggtcgc ggcggaactc gcgcagctcg gcgtggtagc
      121 cgcggatcct gtcgacgggg ctggtcatgg ggtctggagg cggagccggg ggtcgaccgc
      181 atagtaaagc aggtcgaccg cgagattgat gaggacgaag aatagcgcga tcaggcagag
      241 gtaagccgcc atcaccggca catcggcgaa gctgaccgcc tggatgaaga gcaggcccat
      301 gccgggccac tggaacaccg tctcggtgat gatggcaaag gcgatgatcg cgcccagctg
      361 cagcccggtg atggtgatga ccgggaccag cgtgttcttc agggcgtggc cgaaatgcac
      421 cgcgcggtcg gtgaggccgc gcgcgcgggc gaagcggatg tagtcggtgc gcagcacctc
      481 cagcatttcg gagcgcacca gccgctggat cagcgtcatc tggaacagcc ccagcgtgat
      541 cgccggcaag accaatgccc gcagccccga ggccgtgagg aggcccgtgc tccaccagcc
      601 gatctgcacc acgtcgccgc ggccgaagga gggcatccag ccgagctgca ccgagaacac
      661 caggatcagc aggatgccga tgaggaaggt cggcagcgat acgcccgcga gcgatacggc
      721 cagcaggacc tgcgaaaaga acgagcgggg ccgcaatgcg gtgtacacgc ccatgggaat
      781 gcccgccagg agcgcgaaca gcgcggcgac gatcgagagc tcgagcgtcg ccggcagccg
      841 ctccacgatg agcctggaaa ccggctgcac ctggcgcagg ctcaggccga actcgccctg
      901 cagcgcgttg ccgacgaact tcacgtactg cacgtagaac ggctggtcga ggccgagcgc
      961 gcgcgtcacc tcgatgcgct gctcaggggt cgcgtcctgt cccagcatga aggtcacggg
     1021 atcgccgacg aagcggaaca gcgagaaggc gatcaggccc acggtcagca tgacgaggac
     1081 cgcctgggcg atgcgccgca ggatgaacgc agccattttg agagaatgtt acccgatgtc
     1141 tttcgcggaa aagctcatcg gctggcagcg ccgccacggg cgccacggcc tgccctggca
     1201 gggcacgcgc gatccctacc gcatctggct ctccgagatc atgctgcagc agacgcaggt
     1261 cggcaccgtc gttccttact atatgaggtt catcaagagg tacccgacgg tcgaggcgct
     1321 cgccgccgct tcggaggacg aggtcctgca gctctggagc gggctcggct actacgcgcg
     1381 gggacgcaat ttgcgcctcg ccgcacagag cgtcgtttct cgcggcgcgt ttccgcaaac
     1441 cgccgcccag atcgcggagc tgccgggcgt cggccgctcg accgcggcgg cgatcgcggt
     1501 gttcgcgttc ggcgagcgcg ccgcgatcct cgacggcaac gtcaagcgcg tgctggcacg
     1561 ccgctacggg attgcggaca aggatgagct ttggcttctc gcggaaaggc tcttgccgcg
     1621 gaagaacgtc gaaacatata cccaggcgct gatggatctc ggcgcgacgg tctgcacgcg
     1681 caagccggac tgcggcgcgt gtccggtgcg cgcgggctgc gtggcccgca acaccgggcg
     1741 catcgccgag ttgcccgcgc cgcggctgcg caaggcgctg ccgctgaaac aagcgacctg
     1801 gttcgtctat cgggatgcgg gccgggtgct cctcgagcgg cgtccgtcgt cgggcatctg
     1861 gggaggcttg tggtgtttcc ccgagcgtcc actcgtcggg tcgagcgatc cgaaaaagct
     1921 cgcgacgatc gaccacggct tcacgcattt tcggctgcgg atccacccgc tgctgcacga
     1981 agtgaggaaa ggcgcacgca tcgatttccc gggcctgtgg gtcgatctgc acgacgccct
     2041 gcgcgcggcg atcccgacgc cggtaaagaa tctcttacag gaattgcaga gaaacgcgac
     2101 acgcggcagc cgcagccggc gatagcaccg cggcgggcac ggtgtgaaaa tgctcgcatg
     2161 aaaaacgaag ggcgcggccg ctagccgcgc cctccgggat tgcctctcaa gcggcgatcg
     2221 aatcagcgac tacacgacgg agcgggctgc gctccttgtt tggccctacc gggtaccggc
     2281 tcggagtcgg ttgctcggat ggggctggta acagcgggtc ggtcacttcc tcgttcgtgt
     2341 gtagagcatg cgctacacgc gtcgaataat ctacggcagc cccctagtcc ggtcggacta
     2401 gggcgtgcac gcgttccagg cgcaccttgg catcgtccag ctcgagcagc gcctgcttgg
     2461 tctcgagcga cagcggcagc agctcggcga gccgcgaggc cacccacgag cacgagtcca
     2521 ggcggtgcgg ctgctcgatg agccccgggt gctgctcgat tacgcgctcg aggagccgca
     2581 cgcagcggga gagcttttcc ggcgggacgc catcggcttc ctcggccaga agctcgacgc
     2641 tggcgcgcgc gaggccgtcg ggctggaccc ggcgctcgag gatgcgaaag cgccgttccg
     2701 cgcgcgcgat cacctggagc aagccgagct gcggcatgtc ccattcggcg atccgggcga
     2761 ggcagccgac ggccgcgggc agcgccggcg caccgacttc ctttccttcg cggatcaggc
     2821 agacgccgaa gggcgaaccg tccttcaggc acgccttggc catgtccatg tagcgcgcct
     2881 cgaaaatgcg cagcggcagc cggccgccgg ggaacagcac cgtgtggagc gggaagagcg
     2941 ggaggtcttc tatggggggg gttcttcagt caggccgcgg tgacggacga gcaccttcca
     3001 gtccgcccgg aacgcggccg ccagcttctc cgccaggtac accgagcggt ggcggccccc
     3061 ggtgcagccg atcgccacgg tgacatggct gcggttctcg cgcacgatct ccggcagcca
     3121 gcgcgcgagg agcgcgcgca cgtcctccag ccactgctgc gcgagcgaat cgcgcgcgag
     3181 gtagtcaatt accggcgcgt cgcgcccggt cagggggcgc aactgcgcgt cgtagtgcgg
     3241 gttggcgatc atgcgcgcgt cgagcaccca gtcggcgtcc agcgggatgc cctgcctgta
     3301 cgagaacgac tcgaacagca gcgtgagcgc gccggcgccc aggccgagca ggtcgcggat
     3361 ccagttctgc agcacgcgcg gctgcaggtc gctcgtgtcg atgcgctgcc cgagcggcgc
     3421 cacgccctcg aggagcgcgc gctcgcgctc gatcgcttcc ggcagcgaca tgctgcccga
     3481 cagcgggtgg cgccgccgcg tctcggagaa gcgccgcagc agggtcgggg tgctcgcctc
     3541 caggtacagc acccggcact cgaagccgcg gttcttgagg cgctcgatgt tttccggcag
     3601 cgccggaagc gaggcgctgc gcgcatcgac gctcaccgcg acgcgctcgt ggccggcctc
     3661 gagggtgagg aagtcgacca cctcgagcag cagcgtcgcc ggcaggttgt cgacgcagta
     3721 gtagcccgcg tcctccagga ccttcagcgc gatactcttt ccggagccgg aaagtccgct
     3781 gacgacgact aggttttgag gcactcgctc atgaacttct tgcgctcgtc acccttcttg
     3841 ccggccgctt ccttgttgca cttcgtcatg cggtcctgcg gcgagtcctt gaggcagccc
     3901 gacatgaagt ccttgcgcgc gtcgcccttc atccccttgg ccgaggcctg cttgttgcaa
     3961 tcggccatcc gctcctgctg cgcctgctgc gcggccgacg gcgccttgtc cttgaggcag
     4021 ctcgacatga agtccttgcg ctcgtcgcct ttcatgccct tcgccgacgc ccgcttgttg
     4081 cactcggcca tccgttcctg ctgtgccgtg ggttttttct cctgtgcctg ggcggaaaac
     4141 gacagcgcca tgccgatcag gatcgctcga agcaccatca ctcctctcct ttctggattt
     4201 cctgcgtttg ccgcgcgagg aactcggcca tcgagtcgat gccgcgcagc ttgaggattt
     4261 cgttgcgcac cgcggcctcg accagcactg cgaggttgcg gccggcggcc accgggatga
     4321 tgaccttgcg gatggtgacg ccgaggatct cctcgctcag ctcggagagc ggcagccgct
     4381 cggtgggatc gcgcgccgcg ccggccgtgc cgggcgccgg gcgggcgagg tgcgccacca
     4441 gccgcagctt cattttccgc cgcaccgcag tctcaccgaa gatggtgcgg atgttgagca
     4501 cgccgaggcc gcgcacctcg atgaagtcct tgaggagcgc cgggcagcgc ccctcgagcc
     4561 cgccggcgcc gaagcgcgac acttccacca cgtcgtcggc gaccaggccg tggccgcggc
     4621 tgatgagctc cagtccgagc tcgctcttgc cgacgcccga ctcgccggta atcagcacgc
     4681 ccaggccgag cacgtccatg aacacgccgt gcagctcgac ggtctcggcg agcgccttgg
     4741 cgagatagcg cgcgaggcgc tcgatcacgc gcggcgcggg caggggggag gtgaagagcg
     4801 gcgtgcgggt gcgcgtcgcc gactcgacca gcgccagcgg cggcgcgacg ccgtctgcga
     4861 cgatcaccgc ggcgggcgag gactcgagca gcttgtcgac gaagccgccg acgatgccgg
     4921 cctcgtaggc gccgatgacc tggatgctgt tcgggtgggt gaggttgagg tggccgatga
     4981 gggacgcggc ctgcgccgcc tcgcgccgca cggcggcggt gccgccttcg cggcctgcta
     5041 cccaggcgag cgacagcgac tcgcggttgt cgtcatgcag ccgcgcgacg ttgacctgca
     5101 gcatccggct gccaagcggc gatcagctgg tggagggcgg cggcgtcggg cgccgtggtc
     5161 atcgcttcgc gcaacgcctt gtcggagaac atctgcgcga gctccgacag gatctgcagg
     5221 tgcttctcgg tggccttttc cggcaccagc agaacgaaca ccaggctgac ggggttgccg
     5281 tcgggagcgt cgaacggcac cggctgcgcg agccgcacga aggcgccgag cgcttccttc
     5341 aggcccttga tgcggccgtg cgggatggcg acaccctggc ccaggccggt cgagccgagg
     5401 cgctcgcgcg cgaacaggct gtcgtacacc aggctgcgcc cgatgccgtc gaggttctcg
     5461 aacagcaggc ccgcctgctc gaacaggcgc ttcttgctcg acgcctgcag gtcgacgctg
     5521 acgtgcgaga cggacaggat cttggaaacg agggtcatgc tctcttggcg tagagtttat
     5581 ctttgtattt gagaacctgc cggtcaagct tgtccgccag cagatcgatc gcggcgtaca
     5641 ggtcgccttc ctcgctttcg cagtgcaggt ccttgccgct cacgtgcagc gtcacctcgg
     5701 ccttctgcgt cagcttgctc gatgtgagga tgacgtgggc gtcgatcacg tggtcgaaat
     5761 ggcgcgtgac gcggccgatt ttcgcctcca cgtagctgcg cagggcgggg gtcacttcga
     5821 gatggtgccc gctgacgctg agattcatta caaggccttt ctctggttga ccggagggat
     5881 ctgcagcgac tcgcggtact tcgcgatggt gcgccgcgcc accacgattc cctgctcgcc
     5941 gaggatctgc gaaatgcgcg cgtccgacag cggtgccttg gcgtcctcgg ccgcaacgag
     6001 ctgcttgatg agcgcgcgga tcgcggtgga ggaggcggcg ccgcccgcct cggtcgcgac
     6061 gtggctgccg aagaagtact tgagctcgaa ggtgccgcgc ggcgtcgcca tgaatttctg
     6121 cgtcgtgacg cgcgaaatgg tgctctcgtg cagcccgagc gtgccggcga tctcgcgcag
     6181 cacgaggggc cgcatcgcca cctcgccgtg ctccaggaaa tggcgctggc ggtcgacgat
     6241 cgcctgcgac acgcgcagga tggtgtcgaa gcgctgctgc acgttcttga tcagccagcg
     6301 cgcttcctgc agctgcgagg agatgccggc gccgctgcgt gcggccgcgg cgagctcggc
     6361 gtagagccgg ttgatccgca ggcgcggcat cgcgtcggca ttaaggctcg cgcgccacac
     6421 gttgcgcagc ttcttcacga tcacgtccgg gatcacgtag cgcgcctcga ccttggcgaa
     6481 cgccgcgccg gggcgcggat tgagcgcctg gatcagccgt tgcgccgcgc gcagcgcgtc
     6541 gtcgtcggcg ccggtcgcgc ttttgaggcg cgtgtaatcg cgcgccgcga gcaggtcgag
     6601 atgcttgtcg acgatttcga gggcgaggcc gcgcaccagg tcgtccggca gattcttgat
     6661 ttgcagggac aggcactctg cgggggagcg ggcgccgacg ccggcgggct cgaagtgctg
     6721 caggtggcaa agcgcgatgc tcaaatcctc gaggccggct tcggggtcgc ccggaagatg
     6781 cgcggtcagc atcgccgcga tctcctcgag gctctgcgtc aggtagccgt cctcgtcgag
     6841 cgcgtcgatc agcagctcga cgaaggcgcg gtcgcgcggt ccgaggttgg tgagcgagag
     6901 ctgcgcgcgc aggtgatcgc gcagcgtcgg cgtgtccggc gtggcatgcg tgcggtcgcc
     6961 ttcgtcgtcg tcttccgggc cgcgccagtt agaggcgacc tcgctcggcc agtccggggc
     7021 ttccgagccc gggccctcgg aggtgctcgc cggcgccggg gtttccgccg ccggaccctt
     7081 tgcctgaacc tcgacgcctt cctcgtggtc ctcgcgctcg agcgccgggt tctccatgag
     7141 gatccggtcg atctcctggt tgagctcgat cgtggagagc tgcagcagcc ggatcgactg
     7201 ctgcagctgc ggcgtgagcg tcaggtgctg cgaaaggcga aattgaagcg aaggcttcat
     7261 tcgggaagac tagaccccgc tgctacattc ggaaattttc acccagatat actcggcgaa
     7321 cgctgtcatt atagacaatc tcctcgggat ggcccgatgc gagcaccgcc ccctcattga
     7381 taatgtacgc gcgatcgcag atgccgaggg tctcgcggac gttatggtct gttatgagca
     7441 cgccgatgcc gcgctccttc aggaacctaa tgatgcgctg gatgtcgagc accgcgatcg
     7501 ggtcgacccc ggcgaagggc tcatcgagca ggatgaaggc cggccgggtg ccgagcgccc
     7561 gcgcgatctc caggcggcgc cgctctcccc cggagagcga gatcgccgcg tgcttgcgca
     7621 ggtgggcgat gttcaggtcc ttcaggagct cggcgaggcg cggctcgatc tcctccgcgc
     7681 cgagcccctg cagctcgagg atcgcctgca cgttctcctc caccgtcagg cggcggaaca
     7741 ccgagttctc ctgcggcagg taggagatgc ccaggcgcgc gcgccggtgg atcggcaggt
     7801 gcgtgagctc gcggctgtcg aggtggatcg agccggcgtc gaccgggatc agccccacga
     7861 tcatgtagaa gcaggtggtc ttgccggcgc cgttcggccc gagcagcccg accacctcgc
     7921 cgctggacac ttccagcgac acgtcctgga ccacggtacg gttcttgtag cgcttgcgca
     7981 gccgttgcga gcgcagctcg ctcatggacg ggtcattttc ccgcttccgc gctcggcgcc
     8041 ttgggctgga tgatcgcctt gacgcggtcg cccttgcccg cggtcacctg gtagaagtcc
     8101 gagcgctggt cgacgaagat gtagttgccg gcgacctggt cgccgccgcg gttgacgcgg
     8161 gcgttgtcga acagctcgat cttctggttg cggtcgtcga gctcgatgcg cagcgcctcg
     8221 ccctcgaccc agatgccttc cttctcgccc ggcttcggat cctggcgctg gcggaagcgc
     8281 gcgggcttgc cggtcgcggt ggcgaactgg aagccctcgg cgtcctggcg caccaccagg
     8341 cgatcggcgg tgagccggat ggttcccttg gtgacgacga cgttgccctc gaacacgttc
     8401 agccgccgcg cgtcgtcggc gctcatcttg ttcgcctcga cctgggtggg ctggtcgcga
     8461 tccgctttct ccgcgaggac ggaagagcaa gccagcagcg cggccgcaat ccaggctcgt
     8521 ttcattcggc cgggtcgggt gtgaagcgcg ccaccacgtc gctgcgcagg aggagctcgc
     8581 gcgatgcgtt gttgtattcc atgccgcgcc cggtgagcga gcgccggccc tcgacaatct
     8641 tgacctcgcg gtcggtgcgc accagcgcgc ggtcgcgcag cacgtgcagg aattccgtgg
     8701 tcatgcgcgc ctcgggcccc tcggccgagg catcgcgcac cagcaccacg tcgccgtaga
     8761 ggaagatctc gtcgccgtcg cgcgagagct ggccgcgctc ggcgcgcacg gtgtagcgcg
     8821 gctggttcgg cttggtctgc accacgcgcg gcgcgaagat ctcggtggtg tcgtcgtcgg
     8881 ggtagtgcac catgcgctcc gccgacatca cggtctccgc gtcgccgtcg cggttgtagg
     8941 tggtggtggt gaacttcgcc acgatgtagt cggggtcgtg gcggcgcagg gccggcggcg
     9001 cgtcttcctc gcgcaccgtg cgctccaggt agaaggtgag cagcgccagc gcgagcatca
     9061 ggccgagcgg gaacaggcgg gtggtggaga atctcatccg agatacgact gcaatgcctt
     9121 ttccagcgtg cgctgcgcgc gcatcacgag ctcgcacacc tcgcgcgcgg cgccgcggcc
     9181 ggccggcgcc gccgccacgt agtgcacgcg cgcgcgcacc gcttcgggcg cctcgcgcgg
     9241 cgcgcaggcg aagccgcatc gcgtgagcac cggcaggtcg accagctcgt cgcccatgta
     9301 accgatgctt tcaacagtca gatctttttc acgggtgatt ttctggaaag cggccaactt
     9361 gtcctcgatg ccctgcagca cgtccttgat gccgagctcg gcagcgcgca gcgccaccgc
     9421 cgcggagctc cgcccggaga gcaaggcgca cgcgaccccg ctcgccgcga gcatcttcag
     9481 gccgtggccg tcgtgggccg agaacgcctt cagctcctcg cccgccgggc cgtagaggag
     9541 cgtgccgtcg gtgagcacgc cgtcgacgtc gaagatcatg agacgcaccc cgcgcgcccg
     9601 ctcgagcgct tctgcagacg actctttcaa attactctgg cgcggaaaag gtcgtgcatg
     9661 ttgagcgctc ccacgagcgc gccgcgctcg tcgaccacga gcacctgact gaccttgtgc
     9721 tcttccatca catggactgc ctctgccgcc agcgttttgg gccgcaccgt ccgagggctt
     9781 ttcgtcatca catcatccac gagtacctga ttaagctcgg tgctcttttc gattgtgcgg
     9841 cgaaggtcgc catcggtgaa cacgcccacg accaggcccg ccgggtccat gaccgccgtc
     9901 attccgatac ggccacgcga catttcgatt acggcctgtc tgagcggtgt gccgctggcg
     9961 actttcggga tctggtcccc ggtacgcatc acgtcttcta cgtggaggag cgcttgacga
    10021 gccaacgctt ggccgggaag accgccgcgg ggatgggtac gcgcaaagtc cgcggcggag
    10081 aatccgcgcc tgtcaagaag cgctacggcc agcgcgtcgc cgagggcgag ggcagcagtc
    10141 gtactcgcgg tcggagagag attgtgtgga caggcctcct gcgcaacgcc ggcatcgaga
    10201 tgcacgtccg cttcgcgcgc cagcgccgaa tcaggcttgc cggtgagcgc aatcagcttg
    10261 gcgccgcggc gcttgacttg cggcaggatc tgcacgagct cttcgctggc gcccgaatac
    10321 gaaatggcga tgaacacgtc gccggcctcg accatgccga ggtcgccgtg gctcgcctcg
    10381 gcggggtgca cgaagtacgc cggcgtcccg gtgctggcga gcgtcgaggc gatcttgcgg
    10441 gcgatgtggc ccgacttgcc catgccgctc accaccaccc ggcccttgcg cgccgcgatc
    10501 agctcgacgg cgtcgacgaa gcgctgatcc aggcgtgtga tgagtgcgcg caccgcgtcg
    10561 gcctcgatct ccagcacacg ccgcccgagc tccaaggccg agctgccgtg atttgttttt
    10621 cgcactcttg aagctgcctt tatcatccgc ggtgagtata gcccatggat cctagcgaca
    10681 tagccgtcaa cccgggcgtg caatcggtgc tcgtgctgct cgcgtcgagc gtgctcgcgg
    10741 tcgccgcgtg cagaagccta cgcctgccgc cgatgatcgg ctatctcgtc accggcctgg
    10801 cgctcgggcc gcacgccctg ggcgtggtct cggagcgcga agagatcaac cgcctcgccg
    10861 agttcggggt ggtgttcctg atgttctcga tcggcctcga gttcagcctg acgaagctga
    10921 agtcgatgcg gcgcctcgtg ttcgggctgg gccttgcgca ggtcggcgcc acgctcgcgc
    10981 tcgcggtcgc ggcggcggcg gcgctcggcg cgccgtggca gatggggctc gcgctcggcg
    11041 gcatcatggc gatgtcgtcg accgcgatcg tctcgaagct cctcgccgag cgcggcgagc
    11101 tcgacagcgc gcacggccgc gaggtgatcg cggtgctgct gttccaggac ctggcggtgg
    11161 tgccgctgct ggtgctgatc ccggtgctcg ggcagcccgc cgatgcgatc ggcctcgcgg
    11221 tggcggccgc gctcgccaag gccgcggcgg cgctcttcat cgtgatcgtc ggcgggccga
    11281 ggccgatgcg cgcgtggctg cgcaccgtcg cgcgccggcg cacgaacgag ctcttcgtgc
    11341 tgaacgtgct gctggtgatc ctggtcgcgg ccttcatcac cgccgccgcg ggcctgtcgc
    11401 tcgtgctcgg cgcgttcctc gcggggatgc tgatctccga gaccgagtac cgcttccagg
    11461 tcgagcagga catcaagccg ttccgcgacg tgctgctggg cctgttcttc atcaccgtgg
    11521 gaatgatgct cgacctgcgc ctggtcgcgg cgcagttctg gctggtagtg ctgtttctgg
    11581 ttttgctcat gatcgcgaaa ttctttctca ttgccggcct ttccagggta ttcggcgctg
    11641 cgcccggcac ggcgctgcgc gtcgcgctgg cgctcgcgca gggcggcgaa ttcggcttcg
    11701 tgctgctgcc gctcgccggc gccgcgggca tcgtgcccga cgcgctgctg cagccgctgc
    11761 tcgcggcgat gatcgtgtcg atgctgtgta cgccgttcgt gatcgccgcg agcgaccgca
    11821 tcgtgctgcg cctgtcgcgc acggagtggc tgcagcgctc gctcgcgctg caccgcgtcg
    11881 cggtgcagtc gctcgaggcc gagcgccacg tggtgatcgt cggctacggc cgcaacggcc
    11941 agcgcctcgc gcgcctgctc gacgccgagg gcgtgcgcta cgtcgcgctc gacctcgatc
    12001 ctgagcgcgt gcgcgaggcc gccgccgcag gcgacacggt ggtattcgcc gacggcctgc
    12061 ggcgcgaggc actggtcgcg gcgggcatca tgcgcgccgc ggccgcggtg ctcaccttcg
    12121 ccgacgccgc gaccgcggtg cgaatgctcg cgcacatcca cgacctgaat ccgtcgctgc
    12181 cggtgatcgt gcgggcgcgc gacgaggccg acatcgcgcc gctcaccgcg gccggcgcct
    12241 cggaagtggt gcccgaggcg ttcgagtcgg gcctgatgct cgcttcgcac gcgctggtct
    12301 gggtcggcgt gccgctcagc cgcgtcatgc ggcgcgtgag ccaggtgcgc ggcgagcgct
    12361 acggcctgct gcgcggcctg ttccacggcc gcgagcgcga ggacggcgag ggcgatgccg
    12421 gcgcgcagct ccactcggtg acgctcgaac ccggcgcgcg cgccatcggc cgcacgctgg
    12481 aggagctcga cctcggggtt caggtccgcg cggtgcggcg gcgcggcgtc aaggcgaggc
    12541 tcgacgccgc ctcggcgcgc gagctgcagg aaggcgacgt cgtggtgctg ctcggtcctc
    12601 ccgacgcgct tgcggccgcg gaggagcgct tggcggcgcg ctaggagagc gcggtcgctc
    12661 gagcagctcg cgcgcggccg cgtgccgtgc aatgacgcgc gcactcggcc agcggcgcag
    12721 cgcgcgctcg cccatctcca ccgcggccgc ggcatcttcg gcgagcggca aggactggta
    12781 gagccacggc gcgacctgcg cgccgagcgg gccgcgcgcg gctgcgcgca gctccgacag
    12841 agccgccgag acctcctcgg ccggcgcgag cgtgcgcccg ctggcgacga cataggcgcg
    12901 atcgaagcgc tggtagtcga aaagcgacca ggcaagcacg gcgagcgccg ccaggttcac
    12961 cgccagcagg caaagccgca ccggcaccgc cggaagtttc gcggtcgttg ccgaggcgat
    13021 tccggcgatg agcgccgcga ggcagagaac gtgcgcgtac cagagcggct cctcgacgag
    13081 ggcgtgcagg ctgatcgttc cgaccgccgc gacgatccac cactgcggca gcgccgggct
    13141 gccgcgcaga ctggtccacg ctctcgccca ccagcgcaac gccgcgacga caatcaccgc
    13201 ggcgccgacc agcccggttt cggcaagcag ttgcagcacg atgttgtgtg cggaggtcca
    13261 gaccacttcg taggctccca ggagcggcgg caggccggcg gcgaaggcgg cgccggcaaa
    13321 ctctccccag cccacgccga gccagggcga gccggcgaac aggcgccacg cgagccccca
    13381 gagcgcgagc ctcggctcgc ccgtcccgtc ggccagccgg tcgagggcgg acgcacgaat
    13441 gagcgccatc gcgacgagcg tggcgagcgc gaggatcccg gcatgcagcg cgagtcgccg
    13501 ccacggcggc ccgcgatgac gcagggcgaa gccgagcgcg caaatccaga gcgagaacag
    13561 gattgcgctg cgcgactgcg tataggcgct cgacagcacg agcagcgccg ccgcgctcca
    13621 ccaccagtta ccccgcagga ggccgcgcgc gacgagatag agcagcgcgg cctggccgag
    13681 cgccacatac tcggcgaaca ggttgggctg gccgacatga ccgacgatgc gcggcccgga
    13741 agtcgtcccg atgaagccgc tcaggaagcg cggaactccg tagaactgca ggatgccgat
    13801 caccgcgttc gccagcgctc ccgccaggag ggcccaggca aggacatcga ccacgcgctc
    13861 ggcgccgaag cgggcggcaa gcgcgtgccc cagccatgcg agctgcgccg caagcagcac
    13921 gtacgcgacg ccggcaacgg gaagctgcgc gtaggcgggg gggcgcagcg ccgcctgcaa
    13981 gccgagccag cctgcgaaga gaagcagcca gaccgagaga tcgggaaccg cgtccttgac
    14041 cgagcgcgac gatggcagga aggcggctat ggcgagcgtt gcaagaccgg cgccgatcca
    14101 ctcgtcgtag aagctgcgca gcggcggccg gtgctcaggg aagaggaaag ggattgcagc
    14161 gagcagtccc gcgagcgcga gcgagatgcg cgcagggtcg gcgaaaaaaa agccggcgcg
    14221 aggccggctt gtcgcattca aggcgccgcg cgccttacat cgcgcggcac accacgtaga
    14281 tattggttcc ggtctcctgg taccccgagg ggagcgcatc gtcgagatcg gggttggacc
    14341 cggtctgcgc gtcaccgcgc accgtgccct tgtcgggcgc gccatcgtcc atctgcgtgt
    14401 cgacggcgat ggcgatcttg tcgggcaagt tggtcgagca cacgatcagg cccgggatgc
    14461 cgcccatggc cgggccgccg gcggcgtctc cggtctgcac gccgatgatg ccggcgacgg
    14521 cgttgatggg ctgactgtag cccgagcccg agacgaaacc ggcgcgccgc aggtggtccc
    14581 accacttccg cgactcgttg gcatcggtct ggctgttgta cttgcctgcg accacggcat
    14641 taccgtcgcc cgcgaccgca gcaggagcga ctgtccagcg cgtcgccgcg ttgatgtcgt
    14701 cgcccgggat cgcgcgatag cggtcctggt agccgtagta ggcggcggat acgcccgaaa
    14761 aatcggagac cacgttcttg atcttcgcct gggtgatcat ctcctgtccc ttcaggatgc
    14821 cgccgagcag caatccgatg atgaccagca cgatggcgat ctcgaccagc gtgaatccct
    14881 gttgttttgc ctgcgctgct ttcatagccc ctccatgggc gtgaccggtg cctcgtgggc
    14941 cgcaaggagt atgccaaaca gtagtattta aaaaacagta acttactgac gtaaccttat
    15001 caaaaaccgt cagaattccg acaataccgt tgacagccgg acaggcttta gcggttttcc
    15061 ttggcggcgc gcgcctcgag ctcgtccagc cgttgcttga ggaagcggag ctccgccggg
    15121 tcgctgaccg ccccggtttc cagcagtccc gcgatcatga tcttcgccgc ctcgagctcg
    15181 ttcatgtcct cgaggatgaa gatctccatc tgcttcgccc ataggggcac gtcgcgggcg
    15241 gtcgtctggc ggtggagatc atgcgcgtac cggcgcgcga gcggcaggtc tttcagccgg
    15301 tgcttggcga gcagggccgc gtgcgcgagc cagggccagc ggcggttcgg gtcgcgcagg
    15361 aactcttcat ggacaaactc gagcgcgatg cgcagacgca ccggatcggg ggtctcggtg
    15421 tatacgcgcg ccgcggcgaa caacggatac tggctgcgcg gatcgagctc gagaatcgag
    15481 cgcagccagt ccaccagtcg ccggtagtcg agcttctgat agggcgtcgt gttggcgccg
    15541 ccaagatcgt aggactgcag gtagatcatc gtcactcgcg cgagcgcctc ggcttcgccg
    15601 aagctcgcga ggcgcagcgc cggcgcgccc ggcggcggcg gcagatcggc cggggcgacg
    15661 ctgccagcgt cgcgccttgc ctgccaggcg acctgcgcgg caaggctgac ggcgagcagc
    15721 gcgaataccc agcgcggtac cgcggcgagc ggccggtagt cccgcgtcag acgtttctcc
    15781 ggtaaaaatc gaacaggcct gcggcggcca gcactacggt atagagagcc gcgctggcga
    15841 gcgctgcggc atacacgcct gccggcggcg ggtcgtacag cagccaggcg gtgcgggtca
    15901 gcgcatcgag gcgcggcagc accagcgcca cggcgtcgac ggcgtactcg gcgatccggg
    15961 acgcagccga cgcctcggcg agcggcccgc tcgcgatcgc ctggatcgtg ccgatcgagc
    16021 gcccgagcag gtacagcccg ccggttgccg cgatcgcggg cacgaactgg ccgagcgtca
    16081 tggcgaagaa gagcgccgcc gcggccacca gcgcggtttc gcaggcgagc gacaggcccc
    16141 agagggtgac ggcgccgggc tgcgcccaga ggaggagcgg cagggcgaac accaccgcca
    16201 gcgccgcgcc gcaggcgacg aagccggcga ggcgcccgag gtaatgggtg gtgcgcgaca
    16261 gcggcagcga gagcatgagc tcgagcccct tgtcgtggac ctcgcgcagc gtgctggcgg
    16321 tcacctgcgc ggctacgagg aacaccgcgc aggcgcgcag caccgcggcg atcagggccg
    16381 cctggagcgc aaggctctcg gtgagcgcga cttgcgagac gaacgccgcc agcacgaggc
    16441 cgagcgcgac ggccgcggcc gcgagccacg ggagcccgcc gcggcgggct tcgagcagga
    16501 ccgagcgagc gagagtggcg gcgggcacgt cttctgtaga ctagtcgcgg gggagtgctc
    16561 atgcaacccg cggtgctgac gcggctcgcg aacagaacat ggaccttcag ccagcgctac
    16621 tggcgcatcg tggtggtggc gatgctggcg ctcctgcacg tcgcggtatt ccgcggcgtc
    16681 accgacccgt gggcgcgtgc gctgatgctc gcgcatctgg gactgctgct gctgtggcag
    16741 ccgttcctgc gagccgagca gcgcgtctcg gcgacgcaag gcctgatgct ggccctcgtc
    16801 gcgaccggcg tgatgctgtg gctcgactgg tggttcctcg ccttctgggt ggtggtgctc
    16861 gccggcctgg tcggcggcaa ggcgtaccag cagcatgcgc gctggcagcg ccgctgctac
    16921 ctggtggtgc tcgcgtacct gctcgcgctg ctcgccatcg cgatccttcc ggagatcgcg
    16981 ccgcgccgcg agatcgacgc cgagatccgc gactacgcgg agtacctgct gccgctcgcc
    17041 ttcgtgctga tcgcgctgtt tcccgccgag cccgacgcgg ccgaggccgc gcacctcatc
    17101 gacttcttct acagcctgtt cctgatgctg ctgctggtgg tcgtgatcct cggcagcttc
    17161 accttcatga gcttgcggcg cacgccctat ctcgaggcgc tcacctacac cgtgttcatc
    17221 accgcaggcg cgctgctgct cgtggcgctg gcgtggaatc cgcgcgccgg cggcgggctc
    17281 gaggtgttct tcgcgagata cctcttctcg atcggcctgc cggtggaacg gtggctgcat
    17341 tttctcgccg agctgtcgct ggtcgaggcg cgcccggagc gctttctcga ggaagcggtg
    17401 gcggcgctgg tgcgcctgcc ctcggtcgcc ggcgtgcgtt ggcgcgccgg cgaggcgagc
    17461 ggcgagcagg ggagccgcag cgcgcaggcg gtcgactacg cggcgagcga gctcgagctc
    17521 accatctact cgcgctaccg gctcggcgct gcgctgcgct ggcacctgca cctcctcggc
    17581 cagctgctcg gcgagttcca tgtcgcgaag ctgcgcgagg agaagctgcg ccaggcgagc
    17641 tacctgcagg cagtgcacga gaccggcgcg cgcatgacgc acgacatcaa gaacctgctg
    17701 caatccctct cggtgctgac ctcggtcgct gcgcgggacg atagcgggtc ctcgcagctt
    17761 cacggcctgg tgcgccgcca gcttccgctc atcgagcggc ggctcgccga cacgctggag
    17821 aagctgcagc gcccgcaggc caccggcgag acctatgtgg cggcgcgcgg ctggtgggag
    17881 gcgctcgcgc gccagtaccg cggcgagggc gtggagttcg atgcgatcga tccgccgccc
    17941 ggcacgcgcg tgccgcgctc gctcttcgag agcgtcgccg acaacctgat ccgcaatgcg
    18001 ctcgccaagc gcgccgacga cgagggcctg cgggtgcgcg tctcgctcga atgcggcgag
    18061 cgcgtcgcac tgcgcgtgtg cgacagcggg acggcagtgc cgccggagat ctccgccagc
    18121 ctgctgcgcg ctcccgtcag ctcgcgcagc ggcctcggca tcggcttgta ccaggccgct
    18181 cgccaggccg agtcgaacgg ctaccgcctg gagctcgaaa ccaaccgcga cggcgaagtc
    18241 tgcttcgtcc tgaccgggct cgccggctag ggcagccggc ccgccgcgac catgcggctc
    18301 ttcagcacgt tcggcgagac ccaggcgagc acgtcgtcga acgccggctc gtgcagcttg
    18361 tggacgaaga tgccgtcgtt gtcggcattc tccagctcgt cgccagcggc gccggccacc
    18421 tgcgcgccgt ttgtctggaa cgcgccgaag ccgttcttgc cgtgcgagac gaccatggcg
    18481 gcgatgttac tggcgatatc gacgccggcg gcgctcgccc tcaccgtcag gtttccggtg
    18541 tcggtcagcg cgaacgaagc ctgcaggcct ccggtgggat cgtcggcgag catcgccgtc
    18601 acgcgataag tgaagcggcg tccccaagca tcggtctccg ctacgccgag cgccgcccag
    18661 ggaaccacgc cctcgggctg cgtacacgct gttcccgttc tggcctccga acccgcgccg
    18721 gccatggtgt tagcggtggt cgccgccgcc gggcacggca gccggcccca ggtcacggcg
    18781 aatccgagga tcgcttccac gatcaggttc gcctgcttct gcgtctcgtt gatgcggtgc
    18841 tgatcgatct gcgtgttgag cgggaccgcc agcaccccga gcagcagggc gatgaccgcg
    18901 agcgccacgg caagctcgac cagcgtgaag ccgcgcgcgc gcctcatggc ttcaccccga
    18961 ctggcgtgtc attgaagctt gcagtcctgg cattgctttc gaacaccgtg ccgacgcccg
    19021 cgttggtggc ctcgaaaaac tggttgaaat tggccggcaa cggggtcacg ggacgggcga
    19081 tctgcgccgc ggaaggcagc gcgcgtcccg tcatcaccac gaccgcgtgg ttgtcgtcgt
    19141 tggcggctcc ggtattcgtg acggtgaagc aggccggcgc cgccccgccg caggcgtcga
    19201 cgccgtcgat cgcatagccc ggtgagaagg cgtagtaggc gttctggtgc cagttgttgt
    19261 ctcccagcca tcccgtcacc cagctgctga tgggaggggc ggtcaccctg aagtcgagcc
    19321 atcctgcgac caggatcccg ctgtagtcga actccagccg gcgcgcggcc ttgttcaggg
    19381 tccagctcgc gctgccgccg aggctcaacc cgaggagctg gaagttggcc gcctgcggcg
    19441 gatcgataaa acgcgtcgcg acattgtcga tccgcgccga cgcgatgtcc gggacgcaga
    19501 ttccaaagag gcagaccacc aggctgctgc actcgaggat cggcgtgccg tttccggtgc
    19561 agaccacggg cgtcgcgttc gaccagacgg gcgccgcggt ggagaggggc agcaatccgg
    19621 ccgtggtacc gtcggtgccg agctgcgccg gcgtggacga cggatcgttc cagggaacgg
    19681 cgaagggata gaagcccttc accccgctga ccgtcgagtt ctgccaggcg ttgtaatggt
    19741 tgcgcaggtg ctgcgcgagc tcgcgcgcgg cgcgcttttc caccagccgc atgatgtcgt
    19801 cggagaacac cggcatcagc cggtcgttga aggtcccgct gcgctccgcg gatacgaagg
    19861 tgcggtttgc atcggcgttg tcctcgccgg aggcgatgtc gaggaagtcc ctcgggtcgc
    19921 aggccgcgcc gcaacctcgg gtttgcactc tgccgtcggt gcgcgtcagc accgcgccgg
    19981 gcgagaacac gatcgccacg aggtttgtcg ccggcgaggt tccggtcatc gtcagcgaaa
    20041 cgtttgcggc gcggtaggcg gtgtcgctgt tcagcggcac agggccgttg gcgtggaaat
    20101 cgttggacag cgtgtaccac agccgctccc cggcatcgtc ccttagatcg ggcaatccca
    20161 gcgtgaccca gggcaggcgg ccgatgaggc tcgcgcaggc gctgccgatg atgtcctgac
    20221 cgatcacgag cttgccgtcg tcgttcacgt ccggacaggg cagctcgccc ggccgggcgg
    20281 tgtcggcggc ggcataggcg atgagcgcgt ccttggcccg ggcgagcgct tcgttggtgc
    20341 tgcggtcgcg atcgacgcgg accgtagtga ggttgaacga gctgaacgcg aatgcggcgc
    20401 cgcccaggag aagaacggcc accagcacga gggccacgac gccccgctgc cgggatcggg
    20461 tggccggccg cggcttcatt cggcgcgacg gatgcggatc tcgccgtcgc cgatcacgtc
    20521 gcgcacttcg ccggtgccgc ggtcgatgac ctcgccaggc ttcaggcccg cgcgccggcc
    20581 gccgtcgccc accggcaccg agacccggcc ctcctggctg cgaccgatgc gcggcgcatc
    20641 gctcgagcct tcgggaagcg gctcgccgtt cacccagacc gtcgacgggc cgcgcgagcg
    20701 cgtgacatag ccatcgagct tggtcgtcgg cgaggcgacc accgccgccg ccggcttgtc
    20761 gggcacgcgc gcccggcgcc gcgcgtcgag cgccgagcgc tcctccggcg tgaaaaagag
    20821 ccggccgagc tcctgggcaa agcaagtctg cgcggtcatg agaagcaacg cggcgaaaag
    20881 gcgcgtcatc gcttcgctcc ccggtcgacc acggtgatga gatcgatctc gcagctcgcg
    20941 cgcaggcgcg gcgtgatcgt gacgccggtc gccgcctggc cggtacgcgc gagctggcag
    21001 ctcttcaccg aatagtaggc attgcccgag gcgcgcaggt ctgagaggaa gcgcagcagg
    21061 tcctcctcgt gcagcaacgc gagctgcacg cgcatggtgc tcgcgaggaa ctccacgtta
    21121 tccggcttgc ccggcacgga cttgagcatg cgctggcggt cgatgcggta gctgagatcg
    21181 agcagctcgc gctgcgtgcg gatgcgcgtg atggcgtcgg cccattccag gcggcgctcc
    21241 tcgccgagga tgttgaggcc cttcagctgc ttgtagacgt cgagcttctc cttcacctcg
    21301 cgctcctcct ccgcgatgcg ggcgagccgc tcggtgctct gccggcgctc ggcctgcacc
    21361 gcggcgagct ggcgctggtt ggcggtgagc gactggcccg cgagccagat gagcgcggcc
    21421 ccggcgccga gcagcgcggc cgcggcgagc agcggcaacg cgatcttctt cagctcttcg
    21481 cgcgtcaggt tcatggcagt cggcgcgcca gcacgatggt gaagcgcggc gcttcgcccg
    21541 agtccgagcc gccgatgtcg ccggtgaggg ttccttccga ggtgacgtcg aacggcagct
    21601 gcgtgcgcac cagcgtgtag cccgacccgg cgagcgcgcc ggccaggctt tgcacctggg
    21661 cggtgatacc gcggtagtcg ttgcgctggg tcgcgcgcac tcggcccgag atctcgacca
    21721 gcactgcctg ctcgctctgc gcctcgggct tgacgggctg tgccgcaggc ttggcggctt
    21781 gcgccgcggg cttgccgggg cgcccgacgc tccagttcac gctgtcgatc tccacctgcg
    21841 gaaatcgcgc cagaacctgc gacacgtgca cgaacgcggg ctccggggag gcgctcgcct
    21901 cggcgatgcg gcggaactcg agcaccgtca cccgcaggtt ctcggtggtg gtctcggtca
    21961 ccgggaagct cgccgtgatg cgctcgtagc gctgggcggc gaggcttgcc tcgcggctct
    22021 gcgccgccgc gcggctggcg aggtcgtagc tttcgaacca gcgcgtgccg gcgtataccg
    22081 cgcacgcggc gaatccgagc gcgccggcgg cgaccaccgc gcgctgcagc cgccagacaa
    22141 agtagcgccg gcgatcgccg gtgctcgcga actgctcgcg cggaggctgg cgcgcggcga
    22201 gatgcagata gagcgcctcg gccgcagcgc cctccggaac ctggcgcagc ctgaccgccg
    22261 ccagcgcctg cgcgccgtcg atcgtgcgga acacgacgcg cgcgtccgaa accagcgcct
    22321 gctcgaacac ggcgcgctcg ccgcgcggcg cgacgaccag cgccgggatc ggcgggccct
    22381 cgcgcggcag cgcgcgcagc gtcgcgagat actgcacgag gcgcagcgtc tccgagcgcg
    22441 cgaacgcggc gagcgcttgc ggcaccatgt cgacggcgcg ctccaggcgg gcgaagcgca
    22501 gcttgccgtc ctcgacgaag cactgccgca gccccgcgcg gttcgcggtg atcaccagtg
    22561 cgcgcgggcc gcgcgcgccg agccgggcgg cgagcgccgg cgcgagcagc ggcaccgagt
    22621 acacgccggc gagccgcgcg ccggcttcct cgagcgcgtc gagccagggg gcgaactgct
    22681 gcgtgttggt aaaggaggcg agcagcagcc gctcgttgcg ccgctgcgtc ccggcgatcg
    22741 tgccgagcga cagcgctgcg gcgagccggg tgtcgcggta gcgttgcgcg aggcgccgct
    22801 gcacgatcgc ctcgcggtcg ccgccgcgca ggagcggcac ctggtcctcg tggaagtcct
    22861 cgccggcgag atccgcgacc acggcgaaga gggcgccctt gtggccgcgc aggtgctcgc
    22921 ggaacgccgt caccccgagg tcgtcgccgg cgaacttcgc ctcgagctcg agcctgccgt
    22981 gccgcgagca gtagaggtag tggtcctcgg cggtaaagta gagcaggtag cgcgcggcca
    23041 tggggctcag aacttcatct tcgagatggc gtcgtacacc gggccgagca ccgcgagcat
    23101 cacccagccg aggatcgcgc cgagcagcac ggtgagcgcg ggctcgatca tcgcctgcgt
    23161 cctgctgatc gcttcgcgca cctcgcggtt gtagaagtag ctcacgttga ggagcgcggt
    23221 gtcgagcctg ccggtggcct cgccgatgcg cagcatgcgg atcaccagcg gcgggaacac
    23281 ggccaggtcc tggaacgccg cggtcaggtt cttgccctcg gcgatctgct ggccggcgcg
    23341 gcgcagcgcg cgctcgagcg tcaggttgcc gacgatctcc tcgcagctgc ggatcgagtc
    23401 gagcaccgtg atccccgagc tgtacatcat cgcgaagctc gaggcgaagc gcgacaggat
    23461 gatcttgcgc aggatcgggc cgagcagcgg cattgagatc ttgtagtggt cgaccgcgta
    23521 agccacgcgc gggttgctgc gcaccgcgag cttgagggcg ccccacgccg cgaacggggc
    23581 ggcgaggatc gcccaccagt agtccacgaa gaggcccgaa accaccatca ggatgcgcgt
    23641 ctgcagcgga atctcctgcc ccatgttgcg gatgaacgcg gtcatctgcg gcacgaggta
    23701 gatcatcagg aagaacgtca ccaccagcac gatgccgccg acgaacgccg ggtacatggc
    23761 gagcttcttg gtctgcgccg cgagctcgtc ctcccacttg agcgactcgg tgaggctctt
    23821 cagcacctcg gcgagcttgc cggtctgctc gccggagcgc accaggctga tgaacacctt
    23881 gctgaacacg ctcggatagt cggcgagcgc ctgcgatagg ctctgcccgc cctggatcgc
    23941 ctcgatcagg ccggacacca cctcgcggaa gcgcaggttc tccacgctgt cgcgcaggtc
    24001 ggcgaggccc tcgaccagcg gcacgccggc ggaggcgagc tgctcgaggt ggaagcagaa
    24061 gttgatcagg tcctggcgct tgaccttgcc gccacccagc aggcgcgaca tgtcgctcgc
    24121 cggcgcgccg gcgacgaggt cgagctccat gcgggcaagg cgctgctcca ggtcaaacag
    24181 gtttaccgcg tccagccggc cgacgacgct ctttcccccc gcatcgattg ccttatagga
    24241 aaacaggggc gtaagcgttt cctcctctag gccatgcggt ccgtcaggtc gaccacgcgc
    24301 atcacttcct cgagcgaggt ggcgccgctg cgtgcgaggc gcacgccgtc gtcggcgagc
    24361 gtcttgaagc cgctggcgcg cgccgccgtg agaatctcgc gcgcggtcgc gcggcgtgcg
    24421 accagctcgt cgatcccggc gtcgatcttg agcagctcga ggatgctctg gcggccgctg
    24481 tagccctggt actcgcattt ctcgcatccc accgcgcgga acagcgccgc cggctcggtc
    24541 ggcgagaatc ccagcaggcg ccgctccttg acgtcgggat cgtaggactg gcggcaggcg
    24601 cggcacaggc gccgcaccag gcgctgcgcg aggatgccga tgatgtttcc ggccaggatg
    24661 tcgggcacga tgccgatgtc gagcaggcgg gggaacgcgc cgacggcgga gttggtgtgc
    24721 agcgtggtgt agacctggtg gccggtcatc gccgcgcgga acgccatctc ggcggtctcg
    24781 tggtcacgca cctcgcccac caggatgacg tcggggtcct ggcgcatcat cgcgcggatg
    24841 ccgttggcga aatcgagctt ggaggcctcc gacaccgagg tctggcggat cagcgtcatc
    24901 gggtactcga ccgggtcttc cagcgtcatc acgttgacgc cgtcggaatt gatgtggttc
    24961 aggatcgagt agagcgtggt ggtcttgccg ctgccggtcg gcccggtgac caggatgatc
    25021 ccctccgggc gcgcgatcat gcgcttcagg agctcgagct gggtttcctc gagcccgagc
    25081 ttctccagcg gcacgatgcc tttctggcgg tcgagaatgc gcagcaccag gttctcgccg
    25141 tgcgtcgtgg gcatgctcgc gacgcggaag tcgaccacgc ggcccgacat ggtggcggag
    25201 atgcggccgt cctgcggcgc gcgcgtctcc gcgatgttca tcttggccat caccttcagg
    25261 cgcaccgcca tcgccggcca gtaggtcttg tgcagcgagc ggatctggcg cagcacgccg
    25321 tcgatgcggt agcggatgcg caggaagttc tgctcgggct cgaagtggat gtccgagccg
    25381 ccgcgctcca ccgcgtcggc gagcagcgcc gagatcaggc gcaccaccgg ctgcgagtac
    25441 tcgtcgcctt cggagagggt ggcgtagtcg acctcgccgg tctcgatctc cttgagaatc
    25501 ccgtcgatgc cgaactcgtg gccgtagtag tgctcgatcg cgcgctcgat ctccgagtcg
    25561 ccggcgaggc gcagctcgat ctcgtattcg cccttgaggt gcgcgcgcag ctggtcgatc
    25621 gcgacgatgt tgttggtgtc ggcgagcgcc acgatgaact tgcgctgctg gcggtcgagc
    25681 gccaccggga acatgcggtt gcgccgcgcc atctcgcgcg gcagatgctt gagcgccgcc
    25741 gggtcgacga tgatgcgcgc gaggtcgacc gattgcaggc cgagcttttc cgacagcgcg
    25801 tcgcgcagcg tcgcttccga aacgaagccg agctgcacca gcagcttgcc gaccgggatg
    25861 tgctgcttgg tctgctccag cagggcgatg cgcagctggt cctcggtcag gatcccctgg
    25921 tcgatcagga tctggccgag gtggcgcttg gcctgcggcg gagcgggctg gttcatgccg
    25981 gaccgacgat ggtaaggcgc gcgctcacgg ggggggtgtc aacttgttcc gggcccatat
    26041 gagtttgtgt caatgaccga gctggtcgac gcgcgcctgg gcggccgcgg tgtcgaagct
    26101 cgcgccgcgc gtcttggcca gcgcgagcgc gcgccggtag tactcgagcg ccaccttcgg
    26161 ctggcgcagg tgatcgaggc tcaccgcgag gttgtaggcg aagtcggggt tgtcgggctc
    26221 ggcggcgaac gccttgaagt actcctgctg ggcctcggcc cagcgtccct gctgggcgag
    26281 ctggttgccg agggtgaagt tgaggacgtg cgcgcccgga ttgtcggcga gcatcgactt
    26341 gacgcgcgac tcggccgcca ccgggtcgac gcgcccggcg cgcagcgcga gcagcgcggc
    26401 ctgcgcatgc gcgtcgcgcg gatcggcctg cagcagccgc aggtacgccc cttcggcagc
    26461 ctcgagccgc ccgctgcgca cgtcgagcgc cgcgagcccg agcagcgcgt cgcggttcgc
    26521 cggctcctcg cgcagcgcct gctgataatc gctgcgcgcg gtcggcaggt cccccgcctg
    26581 ataagcggca tacgccgagc ccaccttggg gtgcacctgc ggcaccgggc ggcgggtact
    26641 gatctcgctc tcggccggcg ccggcgccgg cgcgcggggc gcgggcgccg gcgcgagcac
    26701 tctgggctgc ggcttcagaa gcggcgtttc tgccaccggc ggagccggtt tggcgggaat
    26761 cgcgggcaca gccggtgccg gtgcgggcgc gggcaggccc ggaatggcag attgcgcggc
    26821 agacgccgcc ggtgcgctcg gccggggcgc agcggcgggg gcggcggcga cctgttcgcc
    26881 cgtgggccgc tgcggatttg tgttgaccag cggcgccggc gggcgcagtt ggatccagaa
    26941 gtagaccacg gtgccgacgg cgaacgcgcc gagcgcgccc atggtgatga agaacggcag
    27001 gcgcgggttg gactccttga acttcgcctc gaataccttg cgggctgccg cccgctcgct
    27061 cgcctggggt tcggcttt
//
LOCUS       DATBSZ010000072        23915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1854433, whole genome shotgun sequence.
ACCESSION   DATBSZ010000072 DATBSZ010000000
VERSION     DATBSZ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23915
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854433"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VIG70_04730"
     CDS             <1..560
                     /locus_tag="VIG70_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8249890.1"
                     /translation="PDPTTVSDIFLRDTSVVPGLIKAWEERRYQPVVIDTTAAALGSG
                     AFTRELERLGGTQLLVHGVHDADGRALTFFTFACRPGSISARQTYLAQLLTPALHASW
                     VRTQLQRTPTSADKASNQGVLTVRELDILKWIYLGKSNFEIGAILKISPLTVKNHVQK
                     ILRKLNVVNRTQAIGKSLELRILNP"
     gene            697..1041
                     /locus_tag="VIG70_04735"
     CDS             697..1041
                     /locus_tag="VIG70_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249891.1"
                     /translation="MFMKLILIGLLFVSGAAVAAEPHSFHGAGAAPAAPPALYSFVDV
                     YRLTVAGAMAGQPPVETAPDAPIRVSTVQSPSNEPRFSISPVPQPDKWLLLIAGFALA
                     GWVAHRRLVHAL"
     gene            1041..1835
                     /locus_tag="VIG70_04740"
     CDS             1041..1835
                     /locus_tag="VIG70_04740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsD family peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY8249892.1"
                     /translation="MKKILLLTLLALPTAACEKAAADRPAAQLAAKVNGTEISIHQLR
                     AGGSGNVAQALEKVIDRELLVQKALADKLDRDPQVVQSIENARRQVLAQAYLDRSVSA
                     AAGSSRDEVRAFYAENPALFAQRRVYRLRELTLSASAETVDLLRAEAAKARDLEELAG
                     WLRRRGAKFRVDGLTQPAEQLPLSQLPQLARMKPGEIAVFATPLGASAIQLVHAEEAP
                     FTEQQAAPLIEQFLAGRKRLETAAAEVKRLREVATIEYLGEFKRGN"
     gene            1835..2581
                     /gene="epsE"
                     /locus_tag="VIG70_04745"
     CDS             1835..2581
                     /gene="epsE"
                     /locus_tag="VIG70_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein EpsE"
                     /protein_id="HEY8249893.1"
                     /translation="MKRIAVGVFLLFLAAPGLAQTEKLGSGDAIRVTVFQQPDLTTEA
                     RVTERGTINVPLVGEVKVEGMSLAEAGKQIAGEFARGQFLKNPQVNVALTTLRSSQVS
                     VLGLVARPGRYALDDTSAQLTDVIAAAGGIAAGGADTVTVLRAGKEHKVDMLAKPFKL
                     QGGDTVHVERAPVFYIYGEVARSGAYPVQPNMTVMQAIVAGGGITPRGSERRLKLRRA
                     GADGKLVETDTSLRDIVKPDDVIYVKEALF"
     gene            2594..4030
                     /locus_tag="VIG70_04750"
     CDS             2594..4030
                     /locus_tag="VIG70_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEY8249894.1"
                     /translation="MDLNQFILALRARRKAFVIAFAATVVTAIAVALILPKKYVATAT
                     VLVETRDEQTMTPIRLTARERAGYLQTQVDLLKSNRVAAQVARDLKLAQRPGVVEAWE
                     QDTGGAGLIEDWIASDLLKNIVIDTSVSSFLLINYPSDNPRYAAEVANGFTKAYLDMV
                     LYLRTEPTREAAAWFDQQLKTLRTQVAQAQTKLNTYQKEKGILAEDARVDVESARLAE
                     LSTQLLAARNATYDAISRQKQASEILEREGSPEAIPDVLANPHIISIKTALGAVEARR
                     AQESTVLGENHPQFLRTAAEVQGLRDKLQVEVKKVVTALGNAVEQQRKRERELQAALE
                     AQNQRLLNLKDFRIDMAAMTRDIESAQRSYDTVLARHMTNKIDASATRANVMLLAPAI
                     EPLKPVHPKVGLIAGLAVVLGVVLAAAIVYVFEMLDRRVRSRADLEARLAVPSLGLLS
                     KWQATGGRLLPAPIPSGARAARALPHPW"
     gene            4043..4933
                     /gene="epsG"
                     /locus_tag="VIG70_04755"
     CDS             4043..4933
                     /gene="epsG"
                     /locus_tag="VIG70_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein tyrosine kinase
                     EpsG"
                     /protein_id="HEY8249895.1"
                     /translation="MLAKTTDNVLPMESVSRPNTGRHLGAILMDEGKLSAADAEQVLK
                     RQRELGWRFGEAAIELNFITDADLREALAKQYEFPYLVSGPEGVSKELIAAWDPFNPV
                     VEELRSVRTQLLVRWFNPGAGRRTLAITSPCAREGRSFIAANLAVVFSQLGQRTLLID
                     ADFRAPRQQSIFNISDRFGLSSALSGRSDLSAAAPVAGITGLAVLPAGPLPPNPLELL
                     SRGAFAALLAKAQSEFDVILIDTPPVTEYSDACAITFRAGDALMISRKDATRMADTER
                     AMRELGDASARVVGSLMNAY"
     gene            4942..5073
                     /locus_tag="VIG70_04760"
     CDS             4942..5073
                     /locus_tag="VIG70_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249896.1"
                     /translation="MKKQKLLLAALALLIGAASAAGSKSPPGSNDMIFHQLSARAAP"
     gene            5070..6509
                     /locus_tag="VIG70_04765"
     CDS             5070..6509
                     /locus_tag="VIG70_04765"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY8249897.1"
                     /translation="MTPASSSLRFDASSAARALLAMLVLAGLAVAGGFALAVGELQAL
                     YVGLSLVACVAILIDYRVGAILLVLLLPISSTAVFPHALMGFTGLNPMNLLLGGTLLS
                     YLLRGRAEHAGPFVPWQVLWLLVVPIAIAGVMGTQHFDDILPEFFDRMAVAFTTWRGY
                     LGVTVVKPVLLVLAALLVAAAVVKARKPEAFVVALAAGACALALLMIGFVAISDIRLG
                     MLASPRARAFFNEIGAHANDFGRVFVTAYALMLFTWWETKDARARLALFIVLGILSLG
                     ILLTFSRNAFLGFFVVNGLFLLWKFNMKKLALALLGTAVAAALAPGAVYRRIMYGFDS
                     GSVDTVSAGRVEGLWLPLLPETLNSPLWGQGLDSIMWSDRLAIGAVGLAGHPHNAYLQ
                     ALLDMGLIGLALLLAYYWHVWNGFRALGSNPYLTPAMRGFFQGACAALVAFAAACMTG
                     GSLRPQPDNVLLWIAIGIMYGLLARKPAS"
     gene            6510..7655
                     /locus_tag="VIG70_04770"
     CDS             6510..7655
                     /locus_tag="VIG70_04770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8249898.1"
                     /translation="MPLAERVRTGSAERSRPAALPARKHLCFVAPRAWPVLSGDPAIP
                     VVGGAEVQQSILARLFASHGHRVSMVCLDYGQPERARIDGIEVFRIFGLKQGLPLLRF
                     LHPRVTSMWRALREVDADIYYCRSVSPWVGVIAEFCRRHGRRAIYAGASDRDFVPGQG
                     GQMRHARDRWLFRRGLAAMDAIVAQNEVQRASCRETYGRDALVIPSCYELPQKRQAAA
                     RDCVLWVGTLHENKRPELLLELAARLPQRRFVLIGGPRQGSEAFYERIRAQAAAAPNV
                     TLTGFLPLAQVEPWFDRAAVLVNTSTYEGMPNTFLQAWARGVPTVATVDVGASVHRVA
                     PAVDQLAEGIEAAFSDPSLGARCLEYFGRQHSSAETLRRYAQVFDGL"
     gene            7652..9088
                     /locus_tag="VIG70_04775"
     CDS             7652..9088
                     /locus_tag="VIG70_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY8249899.1"
                     /translation="MNTAVRAISWNRLKRRALSLGAVKAFDHATHFLLPVVLTRCLDA
                     ATFGQYRLLWLVVGTVMTIATLNMAGGLFYFVPRSDARGKRLYIHQTMLFLAVSGLVC
                     AAVAGPWNPLLPAAVAPLAAYGWLVPAFVALWVASVLLDWLPTIDERIRWQAYATGST
                     AILRVALVAAAGWFTGELRAILWMLLAFVLFKLALLLYYVYRHHGLGRPWLERAAFAD
                     QFAHCAPFGVSNALYSLRAQTDQWVAASLFALSSFAAFSVAAIVGHVVQILRHSVMEA
                     FMPTMSRMQAGGDVRGMMTMNSRANIMVGTLLYPLLAFAFAFAEDIITVVYTAAYLEA
                     APVMRVYIVGMLAMVIEVGSVVLLLRQGPFALRITALALAVSVALSWSAAQYLGLAGA
                     ALGSVAAIYLDRALLLRRIARHTGIALRELQKWGPLLRTLALAAACGLVSWLLVQRFL
                     GAALPMVRLAAGATVLALAYVPLWRAIK"
     gene            9085..10236
                     /locus_tag="VIG70_04780"
     CDS             9085..10236
                     /locus_tag="VIG70_04780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8249900.1"
                     /translation="MRLLFITGSLVHGGAERHTITLLNRLAQRGHECHAAYVRDDVSQ
                     LDRLKGAAGATCLHARRYLDLAALQHLAALIKKLRPSAVVAANPYAMLYASLALRQSG
                     VSAPLVVTFHTTLLVSAKAWLQMLYYRPFFWSADSLVFVCEAQRRHWQRRLVSGRSNE
                     VIHNGVDPQYWRSPGPEVSARMRRVLGFADDDYVIGMCAVLRSEKNPLQLIEALAGLR
                     ARGLAPRALFIGDGPMRAAIEARARALGVAAAVLIAGFQHDVRPLLAAADVVALCSTT
                     ETFSLAALEAMAIGRPVVLSEVGGAAEMVRSGSEGFLFPVGDTRALVDRLAALHAADL
                     RRRTGAAARETVEARFSERAMVDRYETLLQQLVLKRSERENLRRSATAY"
     gene            10202..11227
                     /locus_tag="VIG70_04785"
     CDS             10202..11227
                     /locus_tag="VIG70_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8249901.1"
                     /translation="MKTYEEAQQRIKSRRRTWLVTGAAGFIGSNLAEALLKLDQNVVG
                     LDNFSTGKRENLQDGMRLIEGDIRSLETCREACRGVDVVLHQAALGSVPRSIERPLAT
                     HDNNVNGFLNMLVATRDAGIPRLVFASSSAVFGDDPLLPKVEQRIGRAASPYGLSKHI
                     NELYADVFAACYGFQWIGLRYFNVFGPRQDPDGAYASVIPAWIGALLRGRSAYINGDG
                     SCARDFCHVDNVVQMNLRAAITDDPAAIDQAYNVAHGEMTSLSELFEIIRTLLGRRLP
                     HLKAVRPVYREPRRGDILLSRADIGKAQRLLGYEPTMRIMDGLESTMNWYVASLAPRE
                     ERKVANV"
     gene            11220..12545
                     /locus_tag="VIG70_04790"
     CDS             11220..12545
                     /locus_tag="VIG70_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY8249902.1"
                     /translation="MSEVLEKIDIENQAALPTVAVLGLGYVGLPVAVAFGRQRRTIGY
                     DLSQKRVENLKHGVDVTGEVSGAELSEAKHFRPTAHPGSLQDADFIIVAVPTPINAAR
                     QPDLRPLQSASETVGRHLKQGATVIYESTVYPGCTEEICVPILERHSGMRWRQDFHVG
                     YSPERINPGDKEHSFVRIKKIVSGDDEATLEKVAALYGSVVTAGVYRAPSIRVAEAAK
                     VIENTQRDLNIAFVNELAIIFEKLGIDTQEVLKAAGTKWNFLPFRPGLVGGHCIGVDP
                     YYLTHKAELAGYHPEVILAGRRINDGMGSHIARKTVQQMIHAGRNIKGARVNVLGLTF
                     KEDVPDIRNSKVIDIVRELHEFGVETFVHDPLAGPDEALREYGLKLADWESLPAADAL
                     IVAVAHQGFLRLPHAELMKKIVRGGCVIDVKSVLDAEAFRREGLSVWRL"
     gene            12642..13751
                     /locus_tag="VIG70_04795"
     CDS             12642..13751
                     /locus_tag="VIG70_04795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8249903.1"
                     /translation="MSSSYSRLVMLGAAPETRGSIAAVVESYRAHGLFKRWAIDYLPT
                     HGDGGAARNATLTLDALRRFALLRARHGRMPVHVHMSARGSPARDAAVVAAVLAARSP
                     LVLQLHGAGFERSHGDALMRFLLERAAYVVIPSESLRAWVRSVARGAHAVCLPNPVAC
                     APAAEGAVPPSRSNLVLFLGRMEPAKGVLDLLDALAAVRAVVPDVRLVCAGDGDRLGV
                     AHYAERLGIADAVKFTGWVGPSGKRALFEAAAVFALPSYEEALPISLLEAMAAGVPPV
                     VSAVGGIPEVVADGVSGLLVAPGDKASLERALRRLLADSGLGERIGAAARETARLRFA
                     PERALPRLEEIYASLGVHALEAEIPARVADLRRAA"
     gene            13748..14848
                     /locus_tag="VIG70_04800"
     CDS             13748..14848
                     /locus_tag="VIG70_04800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8249904.1"
                     /translation="MKQPVVLLLGPDRGAISGVSTHLNLLFGSALARRFDLAQFQVGS
                     EGRTEGRIGLLARMAASPFLLAAAILRTRAAIVHVNTSLNTRSYWRDLAYVAAAKLCG
                     ARVVYQIHGGTLQDFYKPVLRATLRWPDVIVVLSRCELEETRAIVPEQSVALVPNAID
                     CTPFQRHRRPAPDPRAPLGLIHIGRLVKEKGVFEMVEGLALARRQGVAASLVIAGDGP
                     ALSGLQERVERLGLAERVSFAGPAFGERKARLLGEADVLLFPTYHREGLPYVLLEGMA
                     AGLAPITTRVAAIPDVITEGLHGLFVPPRDPEAVARAIAALAADRTRLARMSAACRER
                     VAASYSIERLADDFTMLYHKLERKSWAPSQAG"
     gene            14824..16560
                     /gene="asnB"
                     /locus_tag="VIG70_04805"
     CDS             14824..16560
                     /gene="asnB"
                     /locus_tag="VIG70_04805"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY8249905.1"
                     /translation="MGAIAGWVAGRNAPDAAALLPPLETLVHRGGDVGGIFTFQAESG
                     HRVVLGQAHYAEDAGIAVILDGGHAFGGESPAQTLVQAYERWDKEAVRHLRGAFAFAL
                     WDARKERLMLARDRFGEKPLYVCEQGGVLHFASQVKALVKSPGVSARPDPNAVRDYLA
                     YRYVPGPRTLLAGIRKLAPGSYSLWQFGRLREVRYWVSPDKQPQGIRDSADPVAEFTE
                     KLDEAVRQRLPAGVLLSGGIDSAALVALAAKHQKVTTFSAGFADDTQSELAPAARVAK
                     QFGTEHHEIVLSPREVIARLPQVVAERDAPVSRPADVALYFIAAEVARHGGARAVLTG
                     DGADEVLGGYRRYVAERYGWGLRSLPTLLMLAAPLASSRPRLRTALASLRTSDWRERY
                     VRWIGVERNTLLGKTPSGPASKDKPPFDGDPRASSLRRALYFDQSSWLPDNVLERTDR
                     MTMAASLEARAPFLDHRLVEFVSTLPDELRVRGLATKWILRQAARPLLAGLAPRKAGF
                     RIPVGAWLRSELREFLLDHLRGAGSLTRDYYDTAVLDRLLDQHLAGRRNHEEILWTLL
                     NLEIWHRACRPA"
     gene            16509..18539
                     /locus_tag="VIG70_04810"
     CDS             16509..18539
                     /locus_tag="VIG70_04810"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEY8249906.1"
                     /translation="MDASEPRNLAPGLSPGLRRKLAWRLNRLRCMTPAEVANRVLRAL
                     QARAERSGLFGARRVPEPDLAAFSNPWHRCEPGIDPKPYVAAADRIAEGWLDVFTLRG
                     VQVGSPPRWNRDPKTGIEAPLAFGKELDYRDADLVGDIKYLWEPNRHQHLVTLAQAYA
                     LTQKPAYLQAITEHLDSWFLACPYGYGPNWASSLEAALRLINWAAVWQLIGGSQAKEF
                     NTLEGGAFRDRWLKSVYQHARFIRGWLSLHSSANNHLIGEATGLFIAGLAWPHWPAAR
                     GWAATGKLILEREALKQNAPDGVNREQAVWYQQFVLDFMVTALLAGKANGQWFSADYE
                     SRIEAMMDFLASIMDAGGNVPMIGDADDGFVAKLSLEKLPYSSLLALGAILFKRGDFK
                     LKARKLDDRARWLVGAAADAEFERLDAEKTRLPLRQAFPEGGTFVLGCAFETPQEIRV
                     VADAGPLGYRSIAAHGHADALSFTLSAGGREFLIDPGTYAYHTQERWRQYFRGTAAHN
                     TVRIDGLEQSVSGGNFMWLKKARAGCSLWLSSTDQDSFEGWHDGYMRLDDPVKHRRLI
                     QLHKKARQVVIEDTLEMGEEHDVELLFHCSEHCRVDAVPGGYLVSQGEHSIRILLPAI
                     DGATASVQRGSVAPIMGWVSRSFDQRAPTSAIVWRARLSSAARLRTEIAVTQ"
     gene            18624..19937
                     /locus_tag="VIG70_04815"
     CDS             18624..19937
                     /locus_tag="VIG70_04815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY8249907.1"
                     /translation="MKICVLGLGYVGAVSAGCLAKDGHEVIGVDPERAKVDLINAGRS
                     PIIEKDIGGIIQEQVAAGRLSATAEVSHAVRHTDLALVCVGTPSQANGGIDLKFVKRV
                     CEQIGEAIQTHPGAPVIVMRSTMLPGTMREVVTPLLEKCSGKRAGEEFGLCINPEFLR
                     EGTAVHDYYHPPKTVIGEVNRASGDLLAAVYSKMPGPMIRTDIETAEMVKYADNAWHA
                     LKVGFANEVGSLCKAVQVDAHRVMEIFCQDQKLNLSPYYLKPGFAFGGSCLPKDLRAL
                     LYKAKTLDVALPILSSVLPSNQLQIERGVQAVIEKGHREIGILGISFKAGTDDLRESP
                     MVELTERLIGKGYEVRVYDRNVSLACLHGANRDYILNKIPHLSRLMVPTIDEVLGHAR
                     TIVIGNGAPEFGDVPQRISEGQTIIDFVRVSQSRSIAGVYEGLCW"
     gene            19928..20422
                     /locus_tag="VIG70_04820"
     CDS             19928..20422
                     /locus_tag="VIG70_04820"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8249908.1"
                     /translation="MLVALRPPVRFAFAKSKREFGFLERRFTPRTVFMEIGGADCSFA
                     VRAAGYVERVYAIDVSGRFLQSVAAPLNLKLVLCDGVRIPVPEAAVDIAWSGAFMDHL
                     HPDDATGHLRNVRRALADGGEYLCETASPQETRRRMLAAGFCAVRMPLLSRFMKSARI
                     AAIK"
     gene            20434..21642
                     /locus_tag="VIG70_04825"
     CDS             20434..21642
                     /locus_tag="VIG70_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8249909.1"
                     /translation="MPRRVLILVENLPSPFDRRVWQEATTLRDAGYTVSIICPTGRGY
                     EKKYEAVEGIDIWRYDLPTEAEGAIGYALEYFVALSGTFLLSLRVFFGRGFDVIHACN
                     PPDLFFLIGNFFKLFGRKFVFDHHDANPELYEAKFGRRDLFWKAMCLLERMTFRFADV
                     SIATNESYRRIAIERGGMPPERVFVVRSGPSLERLKQGLPVPSLKRGKRYLVGYVGVM
                     GRQEGIDLLLQAVRTLVFDFQRKDIHFGLVGGGTSLEEMQALAISLGVEDYVTFTGRV
                     PDAELLAILNTADVCVNPDVANAMNDISTMNKIMEYMALGKPIVQFELTEGRFSAQDA
                     SLYAKRNDPVDFAEKIVALIDDPEKRKAMGAFGRRRVREELEWRYEAPKLLAAYAALW
                     NPAPRQQPAT"
     gene            21606..22484
                     /locus_tag="VIG70_04830"
     CDS             21606..22484
                     /locus_tag="VIG70_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8249910.1"
                     /translation="MEPRAAPAACDLSRVVETRTAAQVLEHYLIERELAERLRRAPRA
                     ERPRLYREVYDELFRRVPHHPQLRAAQEPEQAGRRRRSVEQQLRFLRRYLAPGKTFME
                     IGAGDCALALRAAAFASRVYALDVSDQITRGARRPPNFELLLTEGARVPVAPGVVDVA
                     LSNQLMEHLHPEDADEQLRGIHRSLAPGGTYLCITPNRLYGPRDVSAHFDAVATGLHL
                     REYAAHELRRLLLEAGFSEVRFYAGGGGRFVRCPYAAVALFEALLERLPQRLRKRLAD
                     NPPARALLGLRVAATK"
     gene            22481..23458
                     /locus_tag="VIG70_04835"
     CDS             22481..23458
                     /locus_tag="VIG70_04835"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY8249911.1"
                     /translation="MSFAGPLFVVGMPRSGTKLLCGLLERHPRLGVTGLETEFLPWLA
                     RRFGRFGDLRRRERFDAFYAEMLRHSYFRYQAERGRLIDAARWHAACADFSAAGVFEA
                     LVRTDAGVPRGSGRIWGDKSPSYIDDLPLLKALYPAAKVLHIVRDVRDYCLSTHEAWG
                     KDMLRAAQRWADDVRKARADGASLGGDYLELRYEGLLAGTEAELRRLCAFLGVEFEPA
                     MLELARSTENLGDARGAQRVVADNHGKFRKKMPARTLARVEEIAGPVLLECGYALELP
                     PRRPRRLSGVEMRLAQLRDAWHLARADRSGWGFWRTALFHLRYFVTTRG"
     gene            23483..>23915
                     /locus_tag="VIG70_04840"
     CDS             23483..>23915
                     /locus_tag="VIG70_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEY8249912.1"
                     /translation="MNYLDQLESESVYILREAFNRIEKLAMLWSLGKDSNTMVWLAKK
                     AFFGHVPFPVLHVDTGKKFPEMYAFRSRYAKEWGLNLIVRPCPPVEEVDPTLPPAARS
                     AARKTLGLKNVMKEFAFQGVIAGIRRDEESIRAKERVFSPRG"
BASE COUNT         3521 a         8432 c         8172 g         3790 t
ORIGIN      
        1 cgccggaccc gaccacggtc tcggacatct tcctgcgcga cacctccgtc gtgccggggc
       61 tgatcaaggc gtgggaggag cggcgctacc agccggtggt gatcgacacg accgccgcgg
      121 cgcttggctc cggcgcgttt acgcgagagc tcgaacgcct cggcggcacg cagctcctgg
      181 tgcacggcgt gcacgacgcc gacggccgcg cgctcacctt cttcaccttc gcctgccgcc
      241 cgggcagcat cagcgcgcgc cagacctatc ttgcgcagct gctcacgccc gcgctgcacg
      301 cctcgtgggt gcgcacgcag ctgcagcgca cgccgacctc ggccgacaag gcctccaacc
      361 agggcgtgct cacggtgcgc gagctcgaca tcctgaagtg gatctacctc ggcaagtcga
      421 acttcgagat cggcgcgatc ctcaagatca gcccgctcac ggtgaagaac cacgtgcaga
      481 agatcctgcg caagctgaac gtcgtgaacc gcacgcaggc gatcggcaag tcgctcgagc
      541 tgcggatcct gaatccctga gaaggcgtcc gagccgttct gcaagaaacg gggccgcgcg
      601 ccccgttttc ttttttaccg atagtccgcc cggagtaccg caggcaacgg ctggaggctc
      661 ggggacgtaa taccccgcct ctagcattgc gggttcgtgt tcatgaaact catcctgatc
      721 ggccttctct tcgtgtccgg cgccgccgtt gctgccgagc ctcactcttt ccatggtgcg
      781 ggcgcagcgc cggccgcgcc accggcgctg tattcgttcg tcgacgtcta ccgcctgacg
      841 gtggcgggcg cgatggccgg ccagccgccg gtcgagaccg cacccgacgc gccgatccgc
      901 gtctcgaccg tgcagtcccc gtcgaacgag ccgcgcttct cgatctcccc ggtgccgcag
      961 cccgacaagt ggctgctcct cattgccggg tttgccctgg ccggctgggt cgcgcaccgg
     1021 cgcctggtgc acgcgctttg atgaaaaaga ttcttctgct cacgctcctc gccctgccga
     1081 cggccgcctg cgaaaaagcg gcggccgacc ggccggccgc gcagctcgcg gccaaggtga
     1141 acggcaccga gatctcgatc caccagctgc gcgccggcgg ctccggcaac gtcgctcagg
     1201 cgctcgagaa ggtgatcgat cgcgagctgc tggtgcagaa ggcgctcgcc gacaagctcg
     1261 accgcgaccc gcaggtggtg cagtcgatcg agaacgcgcg ccgccaggtc ctggctcagg
     1321 cgtacctgga cagaagcgtc tccgccgccg ccggcagctc gcgcgacgaa gtgcgcgctt
     1381 tctatgccga gaatccggcg ctgttcgcac agcggcgcgt ctaccggctg cgcgagctaa
     1441 cgctgtcggc gtccgccgag acggtcgacc ttctgcgcgc cgaggccgcc aaggcacgcg
     1501 acctggaaga gctcgcaggc tggctgcgcc gccgcggcgc gaaattccgg gtcgacgggc
     1561 tgacgcagcc cgccgagcag ctcccgctgt cccaattgcc gcagctcgcg cgcatgaagc
     1621 caggcgagat cgcggtgttc gcgacgccgc tgggcgcttc ggcgatccag ctggtgcacg
     1681 cggaggaggc gccgttcacc gagcagcagg cggcgccgct gatcgagcag ttcctggcgg
     1741 ggcgcaagcg gctggagacc gccgcagccg aagtgaagcg gctgcgcgag gtggccacta
     1801 tcgagtatct aggcgaattc aagagaggta actgatgaaa cgcatagcag tgggagtttt
     1861 tcttctgttc ctggcggctc cggggctcgc gcagaccgag aagctggggt cgggcgacgc
     1921 gatccgcgtc accgtgttcc agcagccgga tctcaccacc gaggcgcgcg tcaccgagcg
     1981 cggcacgatc aacgtgccgc tggtgggcga ggtcaaggtg gaggggatgt cgctcgccga
     2041 ggccggcaag cagatcgcgg gcgagttcgc gcgcggccag ttcctcaaga atccccaggt
     2101 gaacgtcgcg ctcaccacgc tgcgcagcag ccaggtgtcg gtgctcgggc tggtggcgcg
     2161 cccggggcgc tacgcgctcg acgataccag cgcgcagctc accgacgtga tcgccgccgc
     2221 gggcggcatc gccgccggcg gcgccgatac cgtcaccgtg ctgcgcgcgg gcaaggagca
     2281 caaggtcgac atgctggcga agcccttcaa gctccagggc ggcgacacgg tccacgtcga
     2341 gcgcgccccg gtgttctaca tctacggcga agtggcgcgc tcgggcgcct atccggtcca
     2401 gcccaacatg accgtgatgc aggccatcgt cgccggcggc ggcatcacgc cgcgcggcag
     2461 cgagcgccgg ctgaagctgc ggcgcgcggg cgccgacggc aagctggtcg agaccgatac
     2521 cagcctacgc gacatcgtca agcccgacga cgtcatctat gtcaaggaag ctctgttcta
     2581 ggaaggcatg cacatggacc tgaaccaatt cattctggct ctgcgcgcgc ggcgcaaggc
     2641 gttcgtgatc gcgttcgccg cgaccgtcgt caccgcgatc gccgtggcgc tgatcctgcc
     2701 gaagaagtac gtcgccaccg cgaccgtgct ggtcgagacg cgcgacgagc agaccatgac
     2761 cccgatccgc ctgacggcgc gcgagcgcgc gggctacctc cagacccagg tcgaccttct
     2821 caagagcaac cgcgtcgcgg cgcaggtggc gcgcgatctc aagctcgccc agcggccggg
     2881 cgtggttgaa gcctgggagc aggacaccgg cggcgccggc ctgatcgagg actggatcgc
     2941 gtcggacctg ctgaagaaca tcgtcatcga cacctcggtg agcagcttcc tgctcatcaa
     3001 ctatccctcg gacaacccgc gctatgccgc cgaagtggcc aacggcttca ccaaggccta
     3061 tctcgacatg gtgctctacc tgcgcaccga gccgacgcgc gaggcggcag catggttcga
     3121 ccagcagctc aagacgctgc gcacgcaggt ggcccaggcg cagaccaagc tcaacaccta
     3181 ccagaaggag aaaggcatcc tcgccgagga cgcccgagtc gacgtcgagt cggcccggct
     3241 cgccgagctg tcgacccagc ttctggccgc gcgcaacgcg acctacgacg cgatcagccg
     3301 gcaaaagcag gccagcgaga tcctggagcg cgagggctcg cccgaggcga ttccggacgt
     3361 gctggcgaac ccgcacatca tcagcatcaa gaccgccctc ggcgccgtgg aagcgcgccg
     3421 ggcgcaggag agcaccgtgc tgggcgagaa ccatccccag ttcctgcgca ccgcggccga
     3481 agtgcagggc ctgcgcgaca agctgcaggt cgaagtgaag aaggtggtga cggcgctggg
     3541 caacgcggtc gagcagcagc gcaagcgcga gcgcgagctg caagcggcgc tggaggcgca
     3601 gaaccagcgg ctcctcaacc tcaaggactt ccgtatcgac atggcggcga tgacgcgcga
     3661 catcgaaagc gcccagcgct cctacgacac ggtgctggcg cgccacatga ccaacaagat
     3721 cgacgccagc gcgacccggg ccaacgtcat gctgcttgcg ccggcgatcg agccgctcaa
     3781 gccggtgcat cccaaggtgg gcctgatcgc gggactcgcg gtggtgctcg gtgtagtgct
     3841 ggccgccgcg atcgtgtacg tgttcgagat gctcgaccgc cgcgtgcgct cgcgcgccga
     3901 cctcgaggcc cggctcgccg tgccctcgct cggcctgctg tcgaagtggc aggcgaccgg
     3961 cggccggctg ctgccggcgc cgattccttc cggcgcgcgc gccgctcgcg cccttccaca
     4021 tccctggtga ttggtgaacc ccatgctagc caagacgact gacaacgtac tgccgatgga
     4081 aagcgtttcg cgcccgaaca ccgggcggca cctcggcgcc atcctgatgg acgagggcaa
     4141 gctgtccgcg gccgatgccg agcaggtgct caagcgccag cgcgagctgg gctggcgctt
     4201 cggcgaggcg gcgatcgagc tcaacttcat caccgacgcc gatttgcgcg aggcgctcgc
     4261 caagcagtac gagttcccgt acctggtctc cggcccggag ggcgtgagca aggagctgat
     4321 cgccgcctgg gatccgttca acccggtggt cgaggagctg cgcagcgtgc gcacccagct
     4381 cctggtgcgc tggttcaacc ccggcgccgg ccggcgcacg ctcgccatca ccagcccctg
     4441 cgcgcgcgag ggccgcagct tcatcgccgc caacctggcg gtggtgttct cgcagctcgg
     4501 ccagcgcacg ctcctgatcg acgccgattt tcgtgccccg cggcagcaga gcatcttcaa
     4561 catctccgac cgcttcggcc tgtcctcggc gctctccggg cgctcggacc tctcggcggc
     4621 ggcgccggtc gccggcatca ccgggctcgc cgtgctgccg gccgggccgc tgccgcccaa
     4681 cccgctcgag ctgctctcgc gcggcgcttt cgccgcgctg ctggcgaagg cgcagtcgga
     4741 gttcgacgtg atcctgatcg acacgccgcc ggtcaccgag tactcggatg cctgcgccat
     4801 caccttcagg gcgggcgacg cgctgatgat ctcgcgcaag gacgcgaccc gcatggcgga
     4861 caccgagcgc gcgatgcgcg agctgggcga cgccagcgcg cgcgtggtcg gctcgctgat
     4921 gaacgcctac tgaccgggcc ggtgaagaaa cagaagctcc tgctcgccgc cttggcgctc
     4981 ctcatcgggg cggcgagcgc ggccgggtcg aaatcgccgc caggatcgaa cgacatgatc
     5041 ttccaccagc tctccgcgcg ggccgcaccg tgacgcccgc gtcctcgtcg cttcgcttcg
     5101 acgcctccag cgcggcgcgc gcgctgcttg cgatgctggt gctcgccggc ctggcggttg
     5161 cgggcggttt cgcgctggcg gtgggggagc tccaggccct ctacgtcggc ctgtcgctcg
     5221 tcgcctgcgt ggcgatcctg atcgactacc gggtgggcgc catcctgctg gtgctcctgc
     5281 tgccgatctc gtcgaccgcg gtcttcccgc acgccctgat gggattcacc ggcctcaatc
     5341 cgatgaacct gctgctcggc ggaacgctcc tttcgtacct tctgcgcggg cgtgccgagc
     5401 acgccgggcc gttcgtgccg tggcaggtgc tgtggctgct cgtcgtgccg atcgcgatcg
     5461 ccggcgtgat gggcacccag cacttcgacg acatcctgcc ggagttcttc gaccggatgg
     5521 ccgtcgcgtt caccacctgg cgcggctacc tgggcgtgac ggtggtgaag cccgtgctcc
     5581 tcgtgctggc ggcgctgctc gtagccgcgg ccgtggtgaa ggcgcgcaag cccgaggcct
     5641 tcgtggtcgc gctcgccgcc ggcgcgtgcg cgctcgcgct cctgatgatc ggcttcgtcg
     5701 ccatctccga catccggctg gggatgctcg cctcgccgcg cgcgcgcgcg ttcttcaacg
     5761 agatcggcgc gcacgccaac gacttcggcc gcgtgttcgt caccgcctac gcgctgatgc
     5821 tgttcacctg gtgggaaacc aaggacgcgc gcgccaggct ggcgctgttc atcgtgctcg
     5881 gcatcctgtc cctcggcatc ctcctcacgt tctcgcgcaa cgcgtttctg ggctttttcg
     5941 tggtcaacgg gctcttcctg ctgtggaagt tcaacatgaa gaagctcgcg ctcgcgctgc
     6001 tcggcaccgc ggtggcggcg gcgctggcgc ccggggccgt ctaccggcgc atcatgtacg
     6061 gcttcgactc cggcagcgtg gacacggtgt ccgccgggcg cgtcgagggc ctgtggctgc
     6121 cgctgctgcc cgagaccctg aacagcccgc tctggggcca gggcctcgac tcgatcatgt
     6181 ggtcggaccg gctggcgatc ggcgccgtgg ggctggcggg acatccgcac aacgcctacc
     6241 tgcaagccct gctcgacatg ggcctgatcg gcctcgcgct gctgctcgcg tactactggc
     6301 acgtgtggaa cggctttcgc gcgctgggca gcaatccgta cctgacgccg gcgatgcgcg
     6361 gcttcttcca gggcgcttgc gccgccctgg tcgccttcgc ggcggcgtgc atgaccggcg
     6421 ggagcctgcg gccgcagccg gacaacgtgc tgctgtggat cgccatcggc atcatgtacg
     6481 gcctgcttgc ccggaagccc gcgagctaga tgcccctcgc tgagagggtg cgcacgggca
     6541 gcgcagagcg ctcccgcccg gcggccctgc ctgccagaaa gcacctctgc ttcgtcgccc
     6601 cgcgcgcctg gccggtgctc tcgggcgatc cggcgatccc cgtcgtcggc ggggccgagg
     6661 tccagcaaag catcctcgcg cgcctcttcg cctcgcacgg ccaccgcgtg tcgatggtct
     6721 gcctcgatta cggccagccg gagcgcgccc gcatcgacgg catcgaggtc ttcaggatct
     6781 tcggcctgaa gcaaggcctt cccctgctgc gtttcctgca cccgcgcgtc acctcgatgt
     6841 ggcgtgcgct gcgcgaggtc gacgccgaca tctactattg ccgctcggta tcgccgtggg
     6901 tcggcgtcat cgccgagttc tgccgccggc acggcaggcg cgcgatctac gccggggcct
     6961 cggaccgcga cttcgttccc gggcagggcg gccagatgcg ccacgcccgc gaccgctggc
     7021 tgtttcgccg cggccttgcc gcgatggacg ccatcgtggc gcagaacgag gtgcagcgcg
     7081 cgagctgccg tgaaacctac gggcgcgacg cgctggtcat accgagttgc tacgagcttc
     7141 ctcaaaaacg acaggcggcg gcccgggact gcgtcctgtg ggtcggcacg ctgcacgaga
     7201 acaagcggcc ggagcttctc ctcgagctgg ccgctcgcct gccgcagcgg cgcttcgtcc
     7261 tcatcggcgg cccgcggcag ggctccgagg cgttctacga gcgcatccgg gcgcaggcgg
     7321 cggcggctcc gaacgtcacg ctgaccggtt ttctgccgct cgcgcaggtc gagccgtggt
     7381 tcgaccgcgc cgcggtgctg gtgaacacct ccacttacga gggcatgccg aacaccttcc
     7441 tgcaggcctg ggcgcgcggc gtgccgaccg tcgccaccgt cgacgtgggg gcgagcgtgc
     7501 accgcgtggc cccggccgtg gaccagctcg cggaaggcat cgaggctgca ttctcggatc
     7561 cttcgctcgg cgcgcgctgc ctcgagtatt tcgggcgcca gcactcctca gccgagacgc
     7621 tccggcgcta cgcgcaggtg ttcgacggcc tatgaacacc gcggtcaggg cgatctcgtg
     7681 gaaccggctg aagcgccgcg cgctctcgct cggcgcggtg aaggcgttcg atcacgcgac
     7741 gcatttcctg ctgccggtcg tcctgacgcg ctgcctcgac gcggccacct tcggccagta
     7801 ccgcctgctc tggctggtgg tgggcacggt gatgaccatc gccacgctca acatggccgg
     7861 cggcctgttc tacttcgtgc cgcgctcgga cgcgcgcggc aagcgcctct acatccacca
     7921 gacgatgctg ttcctcgccg tctcggggct ggtgtgcgcg gccgtggccg ggccctggaa
     7981 cccgctgctg ccggcggcgg tcgcgccgct cgcggcctac ggctggctgg tgccggcgtt
     8041 cgtcgcgcta tgggtagcct cggtgctgct cgactggctg cccaccatcg acgagcggat
     8101 ccgctggcag gcctatgcga ccggcagcac cgcgatcctg cgggtggcgc tggtcgccgc
     8161 cgccggctgg ttcaccggcg agctgcgcgc gatcctgtgg atgctgctcg cgttcgtgct
     8221 gttcaagctc gcgctgctcc tctactacgt ctaccggcac cacgggctgg gccggccgtg
     8281 gctcgagcgc gcggcgttcg ccgaccagtt cgcgcactgc gcgccgttcg gcgtgtcgaa
     8341 cgcgctctat tcgctgcgcg cgcagaccga ccagtgggtc gcggcgagcc tgttcgcgct
     8401 ctcgagcttc gccgcttttt ccgtcgccgc gatcgtcggc cacgtggtgc agatcctgcg
     8461 ccactcggtg atggaggctt tcatgcccac catgagccgc atgcaggccg gcggcgacgt
     8521 gcgcggcatg atgacgatga acagccgcgc caacatcatg gtgggcacgc tgctctaccc
     8581 gctgctcgcc ttcgccttcg ccttcgccga ggacatcatc acggtggtct acaccgccgc
     8641 ctacctcgag gctgcgccgg tgatgcgcgt ctacatcgtc ggcatgctgg cgatggtgat
     8701 cgaggtcggc agcgtcgtgc tgctgctgcg ccaggggccg ttcgcgttgc gcatcactgc
     8761 gctcgccctc gcggtctcgg tcgcgctgag ctggagcgcc gcgcagtatc tcgggctcgc
     8821 cggcgcggcc ctgggcagcg tggcggcgat ctacctcgac cgcgcgctgc tcctgcggcg
     8881 catcgcgcgc cataccggca tcgcgctgcg cgagctgcag aagtggggcc cgctcctccg
     8941 caccctggcc ctcgcggcgg cgtgcggcct ggtctcctgg ctgctggtgc agcgcttcct
     9001 cggcgccgcg cttccgatgg tccgtctcgc cgcgggcgcc accgtccttg cgctcgctta
     9061 cgtgccgctg tggagagcga tcaaatgagg ctgctcttca tcaccggctc gctggtgcac
     9121 ggcggcgccg agcgccacac gatcacgctc ctcaaccggc tcgctcagcg cggccacgag
     9181 tgccacgccg cctacgtgcg cgacgacgtg agccagctcg atcgcctgaa gggcgcggcg
     9241 ggcgccacgt gcctgcacgc ccggcgctac ctcgacctcg cggctttgca acatctcgcg
     9301 gctctcatca agaaactaag gccttcggcc gtggtcgcgg ccaatcccta cgcgatgctg
     9361 tacgcgtcgc tcgcgctgcg gcaatcgggg gtgagcgcgc cgctcgtggt caccttccac
     9421 accacgctgc tcgtgagcgc gaaagcatgg ctgcagatgc tctattaccg gccgttcttc
     9481 tggagcgccg atagcctggt attcgtgtgc gaggcgcagc gccgccactg gcagcggcgc
     9541 ctggtctcgg ggcgctccaa cgaggtgatc cacaacggcg tcgacccgca gtactggcgc
     9601 tcgcccggcc cggaggtgag cgcgcggatg cgccgcgtgc tcgggttcgc cgacgacgac
     9661 tacgtgatcg gcatgtgcgc ggtgctgcgc tccgagaaga acccgctgca gctcatcgag
     9721 gcgctcgccg gcctgcgcgc gcgcggcctg gcgccgcgcg ccctgttcat cggcgacggt
     9781 ccgatgcgcg ccgcgatcga ggcgcgcgct cgcgcgctcg gcgtcgccgc cgcggtgctg
     9841 atcgcgggat tccagcatga cgtgcggccg ctgctggcag cggccgacgt cgttgccctg
     9901 tgcagcacga ccgagacgtt ctcgctcgcc gcgctcgagg cgatggcgat cggccggccg
     9961 gtggtcctct ccgaggtcgg gggcgcggcg gagatggtgc gctcggggag cgagggcttt
    10021 ctttttccgg tcggcgatac ccgcgccctg gtcgatcggc tggccgcgct gcacgcagcg
    10081 gatctgcgcc gccgcacggg cgccgcggcg cgcgaaacag tggaggcacg cttttccgag
    10141 cgcgccatgg tggaccgtta cgaaacgctg ctgcagcagc tggtcttgaa aaggagcgaa
    10201 cgtgaaaacc tacgaagaag cgcaacagcg tattaagagc cgccgccgca cctggctcgt
    10261 caccggggcg gccggcttca tcgggtcgaa cctggccgag gcattgctga agctcgacca
    10321 gaacgtcgtc gggctcgaca acttctcgac cggcaagcgc gagaacctgc aggacggcat
    10381 gcgcctcatc gaaggcgaca tccgctcgct cgagacctgc cgcgaagcct gccgcggcgt
    10441 cgacgtcgtg ctgcaccagg cggcgctcgg cagcgtgccg cgctcgatcg agcgtccgct
    10501 cgcgacgcac gacaacaacg tgaacgggtt cctcaacatg ctggtcgcga cgcgcgacgc
    10561 cggcatcccg cgcctcgtgt tcgcgagctc gagcgcggtg ttcggcgacg acccgctgct
    10621 gcccaaggtg gagcagcgca tcggccgcgc cgcctcgccg tacggtcttt cgaagcacat
    10681 caacgagctc tacgccgacg tgttcgccgc ctgctacggc ttccagtgga tcgggctgcg
    10741 ctacttcaac gtgttcggcc cgcgccagga ccccgacggc gcctacgcct cggtgatccc
    10801 ggcctggatc ggcgcgctgc tgcgcggccg ctccgcctac atcaacggcg acggcagctg
    10861 cgcgcgcgac ttctgccacg tcgacaacgt ggtgcagatg aacctgcgcg ccgcaatcac
    10921 cgacgacccg gcggcgatcg accaggccta caacgttgcg cacggcgaga tgacctcgct
    10981 cagcgagctg ttcgagatca tccgcacgct gctgggccgg cgcctgccgc acctgaaggc
    11041 ggtgcgcccg gtgtaccgcg agccgcggcg cggcgacatc ctgctctcgc gcgccgacat
    11101 cggaaaggcg cagcgcctgc tcggctacga gccgacgatg cggatcatgg acggcctgga
    11161 gagcaccatg aactggtacg tggcgagcct cgcgccgcgc gaggagagga aggtcgccaa
    11221 tgtctgaggt tctcgaaaaa attgacattg aaaaccaggc ggcgcttccc acggtcgccg
    11281 tcctcggcct gggctacgtc ggcctgccgg tagcggtggc cttcggcagg cagcgccgga
    11341 ccatcggcta cgacctgtcg cagaagcgcg tcgagaacct caagcacggg gtcgacgtca
    11401 cgggcgaggt ctcgggcgca gaactttctg aagcgaagca tttccgcccc acagcgcatc
    11461 ccgggagctt gcaggatgcc gatttcatca tcgtcgcggt gccgacgccg atcaacgccg
    11521 cgcgccagcc cgacctgcgg ccgctgcagt cggcgagcga gaccgtcggc cgccacctga
    11581 agcagggcgc gacggtgatc tacgagtcga ccgtgtaccc cggctgcacc gaagaaatct
    11641 gcgtgccgat cctcgagcgc cactcgggca tgcgctggcg ccaggacttc cacgtcggct
    11701 actcgcccga gcggatcaat cccggggaca aggagcacag ctttgtcaga ataaaaaaaa
    11761 tagtctcggg cgacgacgaa gccacgctcg aaaaagtcgc cgcgctctac ggctcggtgg
    11821 tgaccgcggg cgtgtaccgc gcgccctcga tccgcgtcgc cgaggccgcc aaggtgatcg
    11881 agaacaccca gcgcgacctg aacatcgcct tcgtcaacga gctcgccatc atcttcgaga
    11941 agctcggcat cgacacccag gaggtgctca aggccgcggg caccaaatgg aacttcctgc
    12001 cgttccggcc gggcctggtc ggcggccact gcatcggcgt cgatccgtac tacctcacgc
    12061 acaaggccga gctcgccggc taccaccccg aggtgatcct cgccgggcgg cgcatcaacg
    12121 acggcatggg cagccacatc gcgcgcaaga ccgtgcagca gatgatccac gccgggcgca
    12181 acatcaaggg cgcgcgcgtc aacgtgctcg gcctcacctt caaggaggac gtgcccgaca
    12241 tccgcaactc caaggtgatc gacatcgtgc gcgagctgca cgagttcggc gtcgagacgt
    12301 tcgtgcacga cccgctggcc ggccccgacg aggcgctgcg cgaatacggg ctgaagctcg
    12361 ccgactggga aagcctcccc gccgccgacg ccctgatcgt cgccgtggcg caccaggggt
    12421 tcctgcgcct tccgcacgcc gagctgatga agaaaatcgt gcgcggcggc tgcgtgatcg
    12481 acgtgaagtc ggtcctcgac gccgaggcgt tccggcgcga gggcctcagc gtatggcgcc
    12541 tgtagcgcac gggcgacgcc gccggtttgc ctaggcggcg agcgctgttt catcacgtcc
    12601 ggccgagata atcgccggat gatcagccta caggcgaccc gatgagctcc tcctattccc
    12661 gactggtaat gctgggcgct gcgcccgaaa cccgcggcag catcgccgcc gtggtcgagt
    12721 cctaccgcgc gcacggcctg ttcaagcgct gggcgatcga ctatctgccg acgcacggcg
    12781 acggcggtgc ggcgcgcaac gcgaccttga cgctcgatgc gctgcggcgc ttcgcgctgc
    12841 tccgcgcccg gcacgggcgc atgccggtcc acgtgcacat gagcgcgcgc ggcagcccgg
    12901 cgcgcgacgc tgcggtcgta gccgccgtgc tcgcggcgcg cagcccgctg gtgctccagc
    12961 tccacggcgc cggtttcgag cgctcgcacg gcgacgcgct catgcgcttc ctgctggagc
    13021 gcgcggcgta cgtggtgatt ccgtccgagt cgctgcgcgc ctgggtgcgc agcgtcgcgc
    13081 gcggcgcgca tgcggtgtgc ctccccaacc cggtggcctg cgcgccggct gccgaaggcg
    13141 cggtgccgcc gtcgcgctcg aacctggtcc tgttcctcgg gcgcatggag cccgccaagg
    13201 gcgtcctcga cctgctcgac gcgctcgccg cggtgcgtgc cgtggtcccc gacgtgcgcc
    13261 tggtctgcgc cggcgacggc gaccgcctcg gcgtcgcgca ctatgccgag cgcctgggca
    13321 tcgccgacgc ggtgaagttc accggctggg tcggcccttc cggcaaacgt gcgctgttcg
    13381 aggcggcggc ggtgttcgcg ctgccgtcct acgaagaagc gctgccgatc agcctgctcg
    13441 aggcgatggc ggccggcgtg ccgccggtcg tttccgccgt cggcggcatc ccggaggtcg
    13501 tggcggacgg cgtgagcggc ttgctcgtcg cgccgggcga caaggcgagc ctggagcgtg
    13561 cgctgcgcag gctgcttgcc gacagcgggc tgggcgagcg catcggagcg gccgcgcgcg
    13621 agaccgcgcg cttgcgcttt gcccccgagc gcgcgctgcc caggctcgag gagatctacg
    13681 cctcgctcgg cgtgcacgcg ctcgaggccg agattccggc gcgcgtcgcc gatctcaggc
    13741 gcgcggcatg aagcagccgg tcgtcctgct cctcgggccc gaccgcggcg cgatcagcgg
    13801 cgtgtcgacg cacctcaacc tgctgttcgg ctcggcgctg gcgcgccgct tcgacctggc
    13861 gcagttccag gtgggcagcg aaggcaggac ggaaggccgg atcggcctgc tggcgcgcat
    13921 ggcggcgagc ccgttcctgc tcgccgccgc catcctgcgc acccgcgccg cgatcgtcca
    13981 tgtcaacacc tcgctcaaca cccgctccta ctggcgcgac ctggcgtacg tggcggcggc
    14041 gaagctctgc ggcgcgcgcg tggtgtacca gattcacggc ggcacgctgc aggacttcta
    14101 caagccggtg ttgcgcgcga cgctcagatg gccggatgtc atcgtggtgc tgtcgcgctg
    14161 cgagctggag gagacgcgcg cgatcgttcc cgagcagagc gtcgcgctgg tgccgaacgc
    14221 catcgattgc accccgttcc agcggcaccg gcgcccggcg ccggacccgc gcgcacccct
    14281 cgggctgatt catatcggcc ggctggtgaa ggagaagggc gtattcgaga tggtggaggg
    14341 gctcgccctg gcgcggcggc aaggcgtcgc ggcgagcctg gtcatcgcgg gcgacgggcc
    14401 ggcgctttcg ggtttgcagg aaagggtcga acgcctcggg ctcgccgagc gggtgtcgtt
    14461 cgccgggccg gcgttcggcg agcgcaaggc aaggctgctc ggcgaggccg acgtgctcct
    14521 gtttccgact taccaccgcg agggcctgcc gtacgtgctg ctggaaggga tggcggccgg
    14581 cctcgcgccg atcacgaccc gcgtcgccgc gattccggac gtgattaccg aagggctgca
    14641 cgggcttttc gtgccgccgc gcgacccgga agcggtcgcg cgggcgatcg ccgcgctggc
    14701 cgccgaccgc acgcggctcg cgcgcatgag cgccgcctgc cgcgagcggg tagccgcgtc
    14761 ctattcgatc gagcggctcg ccgacgactt caccatgctg taccacaagc tcgaaagaaa
    14821 atcgtgggcg ccatcgcagg ctgggtagcg gggcgcaacg cacccgacgc ggccgctctg
    14881 ctgccgccgc tcgagacgct cgtgcaccgc ggcggcgacg tcggggggat cttcacgttc
    14941 caggccgaat ccgggcaccg ggtggtgctc ggtcaggcgc actacgccga ggacgccgga
    15001 atcgcggtga tcctcgacgg cggtcatgcc ttcggaggcg aatccccggc gcagacgctg
    15061 gtgcaggcct acgagcgctg ggacaaggaa gccgtgcgcc acttgcgcgg cgctttcgcg
    15121 ttcgcgctgt gggacgcgcg caaggagcgc ctgatgctgg cgcgcgaccg cttcggcgag
    15181 aagccgctgt acgtctgcga gcagggcggg gtgctgcact tcgcctcgca ggtgaaggcg
    15241 ctggtcaaga gccccggggt cagcgcgcgg cccgacccga acgcggtgcg cgactacctc
    15301 gcctaccgct acgtgcccgg cccgcgcacg ctgctcgccg gcatccgcaa gctcgcgcct
    15361 ggcagctatt cgctgtggca gttcggcagg ctgcgcgagg ttcgctattg ggtctctccc
    15421 gacaagcaac cccagggaat ccgcgactct gccgatcccg tggccgagtt caccgaaaag
    15481 ctggacgaag cggtccggca gcggcttccc gccggcgtcc tgctctcggg cggaatcgat
    15541 tccgccgcgc tcgtcgccct cgcagccaag catcagaagg tcaccacgtt ctcggccggc
    15601 ttcgccgacg acactcagag tgagcttgcg ccggccgcgc gggtggcgaa gcagttcggc
    15661 accgagcatc acgagatcgt gctctcgccg cgcgaggtga tcgcgcggct gccgcaggtg
    15721 gtggccgagc gcgacgcgcc ggtctcccgc ccggccgacg tcgcgctcta cttcatcgcc
    15781 gcggaggtgg cccgccacgg cggcgcgcgc gcggtgctga ccggcgacgg cgccgacgag
    15841 gtcctcgggg gctaccgccg ctacgtcgcc gagcgctacg gctggggatt gcgcagcttg
    15901 ccgacgctcc tgatgctggc ggcgccgctc gccagtagcc ggccgcggct gcgcaccgcg
    15961 ctcgccagcc tgcgcaccag cgactggcgc gagcgctacg tgcgctggat aggtgttgaa
    16021 cgaaatacct tgctcggaaa aactccctcg ggtcccgctt ccaaagacaa acctcccttc
    16081 gacggcgatc cgcgcgcgag cagcctgcgg cgcgcgttgt atttcgacca gtcgagctgg
    16141 ctgcccgaca acgtgctcga gcgcaccgac cgcatgacga tggcggcgtc gctcgaggcg
    16201 cgcgcgccgt tcctcgacca tcgcctggtg gagttcgtct ccacgctgcc cgacgagctg
    16261 cgcgtgcgcg gcctcgccac caagtggatc ctgcgccagg cggcaaggcc gctgctggca
    16321 ggcctggcgc cccgcaaggc cggcttccgc attccggtcg gcgcatggct gcgtagcgag
    16381 ctgcgcgagt tcttgctgga tcatttgcgc ggcgcgggct cgctgacgcg cgactactac
    16441 gacaccgcgg tcctcgaccg cctgctcgac cagcaccttg ccggtcggcg caaccatgaa
    16501 gagatcctgt ggacgcttct gaacctcgaa atctggcacc gggcctgtcg cccggcctga
    16561 ggcgcaagct cgcctggcgc ctcaaccggc tgcgctgcat gacgccggcg gaggtggcca
    16621 accgcgtgct gcgcgcgctg caggcgcgtg ccgagcgctc gggcctcttc ggcgcgcgcc
    16681 gcgtgcccga gccggatctg gcggcgtttt cgaatccgtg gcaccggtgc gagcccggga
    16741 tcgaccccaa gccctacgtc gccgcggccg accgcatcgc cgagggctgg ctcgacgtgt
    16801 ttaccctgcg cggggtgcag gtcggctcgc cgccgcgctg gaaccgcgat ccgaagaccg
    16861 gcatcgaggc gccgctcgcc ttcggcaagg agctcgacta ccgcgacgcc gacctcgtcg
    16921 gcgacatcaa gtacctctgg gagccgaacc gccaccagca cctggtcacc ctcgcgcagg
    16981 cgtacgcgct gacgcagaag cccgcctatc tgcaggcgat caccgagcat ctcgatagct
    17041 ggttcctcgc ctgtccctac ggctatggtc cgaactgggc gagctcgctg gaggccgcgc
    17101 tgcggctcat caactgggct gccgtgtggc agctaatagg gggttcgcaa gcgaaggaat
    17161 tcaacaccct ggagggtgga gcttttcgag atcgttggct gaagtccgtc taccagcacg
    17221 cgcggttcat ccgcggctgg ctgtcgctgc actcgtcggc caacaaccac ctgatcggcg
    17281 aggcgacggg cctgttcatt gccgggctcg cctggccgca ctggccggcg gcgcgcggct
    17341 gggccgccac cggcaagctg atcctcgagc gcgaggcgct gaagcagaac gcgcccgacg
    17401 gcgtcaaccg cgagcaggcg gtctggtacc agcagttcgt gctcgatttc atggtgaccg
    17461 cgctgctcgc cgggaaggcg aacggccagt ggttctcggc cgactacgaa tcgcgcatcg
    17521 aagcgatgat ggacttcctc gcctcgatca tggatgccgg cggcaacgtg ccgatgatcg
    17581 gcgatgccga tgacggattc gtggcgaagc tgtcgctgga aaagcttccc tattcgtcgc
    17641 tgctcgccct tggagcgatc ctcttcaagc gcggcgactt caagctcaag gcgcgcaagc
    17701 tcgacgaccg cgcgcgctgg ctcgtcggcg ccgctgccga cgccgagttc gagcgcctcg
    17761 acgccgagaa gacgcggctg ccgctgcgcc aggcgtttcc cgagggcggc accttcgtgc
    17821 tcggctgcgc gttcgagacg ccgcaggaga tccgcgtggt ggccgacgcc gggccgctcg
    17881 gctaccgctc gatcgccgcg catgggcacg cggatgcgct ttcgttcacg ttgtcggccg
    17941 gcgggcgcga gttcctgatc gaccccggga cctacgccta tcacacccag gagcgctggc
    18001 ggcagtactt ccgcggtacc gccgcgcaca acacggtgcg catcgacggc ctcgagcagt
    18061 cggtgtccgg cggcaacttc atgtggctca agaaggcgcg cgccggctgc agcctgtggc
    18121 tgtcgtcgac cgaccaggac agcttcgagg gctggcacga cggctacatg cgcctggacg
    18181 acccggtgaa gcaccggcgg ctgatccagc tccacaagaa ggcgcgccag gtggtgatcg
    18241 aggacacgct cgagatgggc gaggagcacg acgtcgagct cctgttccat tgcagcgagc
    18301 actgccgcgt cgacgccgtg cccggcggct acctcgtcag ccagggagag cattcgatca
    18361 ggatccttct ccccgccatc gacggcgcca ccgcgagcgt gcagcggggc agcgtcgcgc
    18421 cgatcatggg ctgggtgtcg cgcagcttcg accagcgcgc gcccacctcg gccatcgtgt
    18481 ggcgcgcgcg cctttcgagc gcggcgcgcc tgcgcacgga gatcgccgtc acgcaataag
    18541 ccgaccggcg cattaccagc cggactacgc gcaagcgcgc tgccccggcg tccgcttcaa
    18601 gagaaatccc gcatcctcgc tgaatgaaaa tctgcgtgtt ggggctcgga tacgtcggcg
    18661 ccgtatccgc gggatgcctt gcgaaggacg gtcatgaagt gatcggcgtc gatccggagc
    18721 gcgccaaggt ggatctcatc aacgccgggc gctcgccgat catcgagaag gacatcggcg
    18781 gcatcatcca ggagcaggtg gcggcggggc ggctcagcgc caccgccgag gtgtcgcacg
    18841 ccgtgcgcca caccgatctc gcgctggtgt gcgtcggcac gccgagccag gcgaacggcg
    18901 gcatcgatct caagttcgtg aagcgcgtgt gcgagcagat cggcgaggcg atccagacgc
    18961 atcccggcgc gccggtgatc gtgatgcgca gcaccatgct gccggggacg atgcgcgagg
    19021 ttgtcacgcc tctcctcgaa aaatgttccg gtaaacgggc cggagaggaa ttcggcctgt
    19081 gcatcaatcc cgaattcctg cgcgaaggca ccgcggtgca cgactactac catccgccga
    19141 agaccgtgat cggcgaggtc aaccgcgcga gcggcgacct gctcgccgcg gtgtactcga
    19201 agatgcccgg gccgatgatc cgcaccgaca tcgagaccgc ggagatggtc aagtacgccg
    19261 acaacgcgtg gcacgcgctc aaggtcggct tcgccaacga agtcggcagc ctgtgcaagg
    19321 cggtgcaggt cgacgcgcac cgcgtgatgg agatcttctg ccaggaccag aagctcaacc
    19381 tttcgcccta ttacctgaag cccggcttcg ccttcggcgg ctcgtgcctg ccgaaggacc
    19441 tgcgcgcgct gctctacaag gcgaagaccc tcgacgtggc gctgccgatc ctctcttcgg
    19501 tgctgccatc caaccagctg cagatcgagc gcggcgtgca ggccgtgatc gagaagggcc
    19561 accgggagat tggaatactc ggcatcagct tcaaggccgg caccgacgac ctgcgcgaga
    19621 gcccgatggt cgagctcacc gagcggctca tcggcaaggg ctacgaggtg cgcgtctatg
    19681 accgcaacgt gagcctcgcg tgcctgcacg gcgccaaccg cgactacatc ctgaacaaga
    19741 tcccgcatct ctcgcgcctg atggtgccga cgatcgacga agtgctcggc cacgcccgca
    19801 ccatcgtgat cgggaacggc gcgcccgagt tcggcgacgt cccccagcgc atctccgaag
    19861 gccagaccat catcgacttc gtgcgcgtgt cgcagtcgcg cagcatcgcc ggcgtgtacg
    19921 agggcctatg ctggtagcgc ttcgtccgcc ggttcgtttt gcttttgcca agagcaagag
    19981 ggaattcggc tttctcgagc gccgcttcac gccgcgcacg gtgttcatgg agatcggcgg
    20041 cgccgactgc tcgttcgccg tgcgtgccgc ggggtacgtc gagcgggtct acgcgatcga
    20101 tgtctccggt cgcttcctgc agagcgtggc tgcgccgttg aacctgaaac tcgtgctgtg
    20161 cgacggggtg cgcatcccgg tgccggaggc ggccgtcgac atcgcctgga gcggcgcctt
    20221 catggaccac ctgcatccgg acgacgcgac cgggcacctg cgcaacgtgc gccgagccct
    20281 ggccgacggc ggcgagtacc tttgcgagac cgcctctccc caggagacgc ggcgccgcat
    20341 gctcgcggca gggttttgcg cggtgcggat gccgctgctt tcccgcttca tgaagtcggc
    20401 gcgcatcgcc gccatcaagt agcgaaccgc ctcatgcccc ggcgcgtcct gatcctggtc
    20461 gaaaacctgc cgagcccgtt cgaccggcgg gtgtggcagg aggccaccac gctgcgcgac
    20521 gccggctaca cggtgtccat catctgcccg accggccgcg gctacgagaa gaaatacgag
    20581 gcggtcgagg gcatcgacat ctggcgctac gacctgccga cggaggccga gggcgcgatc
    20641 ggctatgcgc tcgagtattt cgtcgccctg tccgggacct ttcttctcag cctgagggtg
    20701 ttcttcggcc gcggcttcga cgtgatccac gcctgcaacc cgccggatct cttcttcctg
    20761 atcggcaact tcttcaagct gttcggccgc aagttcgtgt tcgaccacca cgacgccaac
    20821 ccggagctct acgaggccaa gttcggccgc cgcgacctgt tctggaaggc gatgtgcctg
    20881 ctcgagcgca tgaccttccg tttcgccgac gtgtcgatcg ccaccaacga gtcctaccgc
    20941 cgcatcgcca tcgagcgcgg cggcatgccg ccggaacgcg tgttcgtggt acgcagcggc
    21001 ccgagcctcg agcgcctcaa gcagggcctt cccgttccct cgttgaagcg tggaaagcgc
    21061 tacctcgtcg gctatgtcgg cgtgatgggg cggcaggaag gcatcgatct gcttctgcaa
    21121 gcggtacgaa ctctggtttt cgattttcaa agaaaagata ttcatttcgg cctggtcggc
    21181 ggcggcacct cgctcgagga gatgcaggcc cttgccatct ccctcggcgt cgaggactac
    21241 gtgaccttca ccggccgcgt ccccgacgcc gagctgctcg cgattctgaa cacggcggac
    21301 gtgtgcgtga accccgacgt ggcgaacgcg atgaacgaca tctcgaccat gaacaagatc
    21361 atggagtaca tggcgctcgg caagccgatc gtgcagttcg agctgaccga gggacgcttc
    21421 tcggcccagg acgcctcgct ctacgcgaag cgcaacgatc cggtggactt cgccgagaag
    21481 atcgtcgccc tgatcgacga tcccgagaag cgcaaggcga tgggcgcttt cggccggcgg
    21541 cgggtgcgcg aagagctcga gtggcgctac gaggcgccca agctgctcgc cgcgtacgcc
    21601 gcgctgtgga accccgcgcc gcgccagcag cctgcgacct gagccgcgtg gtcgagaccc
    21661 gcaccgcggc gcaggtactg gagcactacc tcatcgagcg cgagcttgcc gagcggctgc
    21721 ggcgcgcgcc gcgggcggag cggccgcgcc tctaccggga ggtgtacgac gagctgttcc
    21781 gccgcgtgcc gcaccatccc cagctgcgcg cggcgcagga accggaacaa gccgggcgac
    21841 ggcggcgcag cgtcgagcag cagcttcgat ttctgcgacg ctaccttgct cccggcaaga
    21901 cgttcatgga gatcggcgcc ggcgattgcg cgcttgccct gcgggccgcg gcgttcgcga
    21961 gccgcgtcta cgcgctggat gtgtcggacc agatcacgcg gggcgcgcgc cggccgccga
    22021 acttcgagct tctgctgacc gagggcgccc gcgtcccggt tgcccccggt gtcgtggatg
    22081 tcgccctcag caatcagttg atggagcatc tccaccccga ggacgccgac gagcagctgc
    22141 gcgggatcca tcgcagcctg gcccccggcg ggacctacct ttgcattacc ccgaaccggc
    22201 tgtacgggcc gcgcgacgtc tcggctcact tcgacgccgt ggcgaccggg ctgcacctgc
    22261 gcgaatacgc ggcgcacgag ttgaggcggc tgctgctcga agccggcttt tccgaggtgc
    22321 gcttctacgc cgggggcggc gggcgattcg tccgctgccc ctacgctgcg gtggcgctgt
    22381 tcgaggcgct tctcgagcgc ctgccgcagc ggctgcgaaa gcgcctcgcc gacaaccccc
    22441 cggcgcgggc cctgctgggc ctgcgcgttg ccgcaaccaa atgagcttcg ccggaccgct
    22501 cttcgtcgtg ggcatgccgc gctcgggcac gaagctgctg tgcgggttgc tggaacggca
    22561 tccgcggctt ggcgtaaccg gcctcgaaac cgagttcctt ccctggctgg cgcgccgctt
    22621 cggccgcttc ggcgacctgc ggcggcgcga gcgcttcgat gcgttctatg cggagatgct
    22681 gcgccacagc tacttccgct accaggccga acgcggccgt ctgatcgacg cggcccgctg
    22741 gcacgcggcg tgcgccgatt tctccgccgc tggagtgttc gaggcgctgg tgcgcacgga
    22801 tgccggcgtt ccgcgcggca gcggccgtat ctggggcgac aagtcgccct cgtacatcga
    22861 cgatctgcct ctgctcaagg cgctctatcc ggcggcaaag gtgctgcata tcgtgcgcga
    22921 tgtgcgcgac tattgcctgt cgacgcacga ggcctggggc aaggacatgc tgcgcgctgc
    22981 gcagcgctgg gccgacgatg tgcgcaaggc gcgggcggac ggggcgtcgc tcggcggcga
    23041 ctacctcgag cttcgctacg agggcctgct cgccggcacc gaggccgagc tgcggcgcct
    23101 gtgcgcgttc ctcggcgtcg agttcgagcc cgcgatgctg gagctcgccc gctccacgga
    23161 gaacctgggc gacgcccgcg gcgcgcagcg cgtggtcgcc gacaaccacg gcaagtttcg
    23221 caagaagatg ccggcgcgca cgctcgcgcg ggtcgaggag atcgcgggcc cggtgctgct
    23281 cgagtgcggc tacgcgctgg agctgccgcc gcggcggccc cggcggctgt cgggcgtgga
    23341 aatgcgcctc gcccagctca gggacgcctg gcacctcgcg cgcgccgacc gcagcggctg
    23401 gggattctgg cgcacggcgc tgttccatct gcgctacttc gtcaccacgc gcgggtaacg
    23461 ggccgccact agactggcct tcatgaacta tctggatcag ctcgaaagcg agagcgtcta
    23521 catcctgcgc gaagcgttca accgcatcga gaagctggcg atgctgtggt cgctcggcaa
    23581 ggactcgaac acgatggtgt ggctcgcgaa gaaggcgttc ttcggccacg tgccgttccc
    23641 ggtgctgcac gtcgataccg gcaagaaatt ccccgagatg tacgctttcc gcagccgcta
    23701 tgcgaaggaa tggggcctga acctgatcgt gcgcccctgc ccgccggtcg aggaggtcga
    23761 cccgaccctg ccgcccgcgg cgcgctcggc agcgcgcaag accctcggct tgaagaacgt
    23821 gatgaaggaa ttcgccttcc agggggtcat cgccggcatc cggcgcgacg aggagtcgat
    23881 ccgcgccaag gagcgcgtgt tcagcccgcg cggcg
//
LOCUS       DATBSZ010000073         3937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_897999, whole genome shotgun sequence.
ACCESSION   DATBSZ010000073 DATBSZ010000000
VERSION     DATBSZ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897999"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VIG70_04845"
     CDS             complement(<1..1052)
                     /locus_tag="VIG70_04845"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEY8249913.1"
                     /translation="MAALRVRAPHAGQRRSRAARRRLRQGFRLKTQIYITGLIGAVLV
                     LAVTWFLSTHDRVTRQEWVGPSGEARLRDFLAAERFAERMAFEVAEVRSLPELDALPP
                     GGVLLVPNRRQELQAARIADLVRWAERGGHLIAEAEFLGVPDPLLDQLGVKRSQAEAQ
                     RKPLAVQLPGQSRKLSVYFDAAMKLEAPQGDMRLSAGTHLVSLARGRGMVTVATSLRF
                     ARNPGWDDHFAKLRRRPARSIGAGDHAELLLHVLSLTPGRELLVYFQPERLSLWGFLK
                     ENAAAALAAGGLLLALWIWSIVPRFGPVAPDAPPGRRRLLDHLRASGRYYWVKGLRAR
                     LVTAARDAALRRITRA"
     gene            complement(965..2380)
                     /locus_tag="VIG70_04850"
     CDS             complement(965..2380)
                     /locus_tag="VIG70_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249914.1"
                     /translation="MQLAEIAISLRRRSPWEAMDLGLTMLQRWWRQVYLPHLLVAVPL
                     MAAAIAIGWAFERAWVAILIVWWLKPLLDRVVLHVLSRAVFGELQSTRAVLRAAREWL
                     GTGLLMALTFRRFDAARAFNLPVRQLEGQAGRAARERRAVLGRRVGAYATLLTLVCFA
                     LEVIVLWTTFGLLAEIFLPAKADQGKSILEVLFGDTDNAAAVFTFEDAFAYPLAVLIL
                     EPFYVAAGFALYLNRRTLLEGWDIEVALRRIAQRHAAALLFLCCALFAFVMPRDAQAQ
                     EKDPKTEIHEVLKAKEFGYYREVKRWEAILRGEDSGTDLTWLQAIAYALAKIGEIALW
                     VGAGALIAYALWWASRMVPRAGAPAPEPYQPPAALFGMDLAPEKLPPDVPAAAAALAR
                     EGRLREALGLLYRGALSELVHRRGVQLLASHTEGEAVRLARLPYFEVLVDAWQRCAYA
                     RRMPASAEVEQLAAGYGKAFA"
     gene            complement(2367..3320)
                     /locus_tag="VIG70_04855"
     CDS             complement(2367..3320)
                     /locus_tag="VIG70_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEY8249915.1"
                     /translation="MKQTPFERAHASEWEAFEAFLDDRKSFDPAEMPARFRRVCQALA
                     LAAQRCYSADLVDRLNRLALRGHHALYVNRRRESRQVLDFMAGGFPRLVRQEWRVVAA
                     AALLFFGPLLGFIALLQFFPDFVHYLLSPRQIAGFNEMYDPANRRLGMREADTNIAMF
                     GFYIWNNVRIGFQTFAGGMLAGVGTAWFLGANGVIIGAVAGYLTQVGYHATFWSFVAG
                     HSSLELVAIVLSGAAGFKLGLAIIAPGNRSRKSALEAAAQPAVRIMYGAAVMFFAAAF
                     VEAFWSPLTEIPFDLKIAVGLAGWAILLAYFALAGRGRAAR"
     gene            complement(3317..>3937)
                     /locus_tag="VIG70_04860"
     CDS             complement(3317..>3937)
                     /locus_tag="VIG70_04860"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY8249916.1"
                     /translation="PVPRALAWAIDFLLRAALVLMVQMIAAFFGRAGMGVVLIAAFFA
                     EWLLPAWFEARWGGQTPGKRMLGIAVLNDDGTPLRWPGALTRNLLRAADFLPFFYGVG
                     LIAMMANRDFKRLGDLAAGTVVVYRAPATETARKIPEAAPVVPPVELHPEEQRAVLEL
                     AERSATLTRERFEELAALPAPLVGRLEGERAAARILGVANYLAGRQ"
BASE COUNT          644 a         1379 c         1419 g          495 t
ORIGIN      
        1 tgcgcgcggg tgatgcggcg aagcgccgca tcgcgcgcgg cggttaccag ccgcgcgcgc
       61 aagcccttca cccagtagta gcgcccgctc gcgcgcaggt ggtcgagcag gcggcggcgc
      121 cccggcggcg cgtctggcgc caccggcccg aagcgcggca cgatgctcca gatccagagc
      181 gcgagcagca ggccgccggc ggcgagggcg gcggcggcgt tctccttcag gaatccccac
      241 agcgacaggc gctcgggctg gaagtagacc aggagctcgc gccccggcgt gagcgacagc
      301 acgtgcagga ggagctccgc gtgatcgccg gcgccgatgg acctcgccgg gcggcgacgc
      361 agcttggcga agtgatcgtc ccagccgggg ttgcgcgcga agcgcaggct ggtcgcgacg
      421 gtgaccatgc cccggccgcg ggctagcgag acgagatgcg tgccggcgct caggcgcatg
      481 tcgccttgag gagcctcgag cttcattgcg gcgtcgaagt agacgctcag cttgcggctc
      541 tgcccgggca gctggaccgc gagcggcttg cgctgcgcct ccgcctgcga gcgcttgacg
      601 ccgagctggt ctagcagcgg gtcgggcacg ccgagaaact cggcctcggc gatgagatgg
      661 ccgccgcgct ccgcccagcg caccaggtcg gcgattcttg cggcctgcag ctcctgcctg
      721 cggttgggaa ccagcagcac gccgccgggg ggcagcgcat cgagctcggg cagcgagcga
      781 acttcggcta cctcgaacgc catgcgctcg gcgaagcgct cggcggcgag gaagtcgcgc
      841 agccgcgcct cgccgcttgg gccgacccac tcctgcctcg tcacccggtc gtgggtcgag
      901 agaaaccagg tgacggcgag caccagcacg gcgccgatga ggcccgtgat gtagatctgc
      961 gttttcaggc gaaagccttg ccgtagccgg cggcgagctg ctcgacttct gcgctggccg
     1021 gcatgcggcg cgcgtacgcg cagcgctgcc acgcgtcgac caggacctcg aaatagggca
     1081 gcctggccaa tctcacggct tcgccttccg tgtggcttgc gaggagctgg acgccgcgcc
     1141 tgtgcacgag ctcggagagc gcgccgcgat acagcaggcc gagcgcctcg cgcagccggc
     1201 cttcgcgcgc gagcgccgcc gccgccgcgg ggacgtccgg cggcagcttc tcgggcgcga
     1261 ggtccatccc gaacagcgcg gccgggggct ggtaaggctc gggcgccggc gcgccggcgc
     1321 gcggcaccat gcgcgacgcc caccacaagg cataggcgat gagcgcgccg gcgccgaccc
     1381 acagcgcgat ctcgccgatc ttcgcaagcg cataggcgat ggcctgcagc cacgtgaggt
     1441 cggtgccgct gtcctcgccg cgcaggatgg cctcccagcg cttcacttcc ctgtagtagc
     1501 cgaattcctt cgctttcagc acttcgtgga tctcggtctt ggggtccttc tcctgggcct
     1561 gggcgtcgcg cggcattacg aacgcaaaca acgcgcagca caggaacagc aacgcggccg
     1621 catgccgctg cgcgatgcgt cgcaaggcga cctcgatgtc ccagccttcc aggagcgtgc
     1681 ggcggttgag gtagagcgcg aagccggccg cgacgtagaa cggctcgagg atcagcaccg
     1741 cgagcggata ggcaaacgcg tcctcgaagg tgaacaccgc ggcagcgttg tcggtgtcgc
     1801 cgaacagcac ctcgaggatg ctcttgccct ggtccgcctt ggcgggcagg aagatttccg
     1861 cgaggaggcc gaacgtggtc cagagcacga tgacctcgag ggcgaagcac accagcgtca
     1921 gcaacgtggc gtaggccccg acgcggcggc cgagcaccgc gcggcgctcg cgcgcggcgc
     1981 ggccggcctg gccctcgagc tggcgcaccg ggaggttgaa ggcgcgcgcg gcgtcgaagc
     2041 gccggaaggt caacgccatg agaagccccg tgccgagcca ttcgcgggcg gcgcgcagca
     2101 ccgcgcgggt gctttgcagc tccccgaaca ccgcgcgcga gagcacgtgc agcaccacgc
     2161 ggtcaaggag gggcttcagc caccagacga tgagaatcgc gacccaggcg cgctcgaagg
     2221 cccagccgat cgcgatcgcc gccgccatca gcggcaccgc aaccagcagg tgcggcaggt
     2281 acacctggcg ccaccagcgc tgcagcatgg tgaggccgag gtccatcgcc tcccacggcg
     2341 agcgccggcg cagcgaaatg gcgatctcag cgagctgcac ggccgcgtcc cgcaagggcg
     2401 aagtaggcga gcaggatggc ccaacccgca aggccaacag cgatcttgag atcgaagggt
     2461 atttcggtca gcggcgacca gaacgcttcc acgaacgcgg cggcgaagaa catgaccgct
     2521 gcgccgtaca tgatgcgcac cgcgggctgc gccgccgcct cgagcgcgct tttccggctg
     2581 cggttgcccg gggcgatgat cgcgaggccg agcttgaagc cggcggcgcc cgacagcacg
     2641 atcgccacga gctcgagcga cgagtgcccg gcgacgaacg accagaaggt cgcgtggtag
     2701 ccgacctgcg tgagatagcc cgcgaccgcg ccgatgatga cgccgttcgc gccgaggaac
     2761 cacgccgtgc cgacgccggc gagcatgccg ccggcgaagg tctggaagcc gatgcgcacg
     2821 ttgttccaga tgtagaagcc gaacatcgcg atgttggtgt cggcctcgcg catgcccagg
     2881 cgccggttcg ccgggtcgta catctcgttg aaccccgcga tctgccgcgg cgagagcagg
     2941 taatgcacga aatccgggaa gaactgcaag agcgcgatga agccgaggag cggcccgaag
     3001 aagagcagcg ccgccgcggc gaccacgcgc cactcctggc gcaccaggcg cggaaagccg
     3061 ccggccatga agtcgagcac ctgccgagac tcgcgccgcc ggttcacgta gagcgcgtgg
     3121 tggccgcgca gcgcgagccg gttcagccgg tcgacgagat cggcgctgta gcagcgctgc
     3181 gcggcgagcg cgagcgcctg gcacacccgc cggaagcgcg ccggcatctc ggcaggatcg
     3241 aacgatttcc tgtcgtcgag gaacgcctcg aacgcctccc attcgcttgc gtgcgctcgc
     3301 tcgaacggcg tctgcttcat tgccgaccgg ccaggtagtt cgccacgccg aggatccgcg
     3361 ccgcggcgcg ctcgccttcg aggcggccga cgagcggcgc cggcagcgcc gccagctcct
     3421 cgaagcgctc gcgggtgagc gtcgccgagc gctcggcgag ctcgagcacc gcgcgctgct
     3481 cttccggatg gagctcgacc gggggaacga ccggcgcggc ttcggggatt tttctcgccg
     3541 tttcggtcgc gggggcccgg tagacgacca ccgtgccggc cgcgaggtcg cccaggcgct
     3601 tgaagtcgcg gttcgccatc atcgcgatca ggccgacgcc gtagaagaac ggcaggaagt
     3661 ccgctgcgcg cagcaggttg cgggtcaggg cgccgggcca gcgcagcggc gtgccgtcgt
     3721 cgttcaggac cgcgatgccg agcatgcgct tgcccggggt ctggccgccc caccgggcct
     3781 cgaaccatgc cggcagcagc cactcggcga agaacgcggc gatcagcacc acgcccatgc
     3841 cggcgcgccc gaagaacgcg gcgatcatct gcaccatcag caccagcgcc gcacgcagca
     3901 ggaagtcgat cgcccaggcg agcgcgcgcg gcaccgg
//
LOCUS       DATBSZ010000074        19698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_693893, whole genome shotgun sequence.
ACCESSION   DATBSZ010000074 DATBSZ010000000
VERSION     DATBSZ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693893"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VIG70_04865"
     CDS             complement(<1..288)
                     /locus_tag="VIG70_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY8249917.1"
                     /translation="MTGEELPKARIRRRRLFRLVWVVPVIALAVGVYLVWQHMRSLGP
                     EIAIRFDDAAGLRVGQTPVIYRGVQIGEVTAIKLSDDRKQAVVKARLHDSAA"
     gene            complement(285..890)
                     /locus_tag="VIG70_04870"
     CDS             complement(285..890)
                     /locus_tag="VIG70_04870"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEY8249918.1"
                     /translation="MAQESVVSCDTCGLVQTVEGLVPGTAAECIRCGSFVAARSSTRS
                     LEVTAALSIAALILYVPANVYPIMRMYLYGGYSESTVWDGVVTLVRTDQWVVAVIVFL
                     ASMVIPVVKLAGLFSLVVTAWMGWGRRLRSRTQLYKFIDAIGPWAMLDVFLLAVLVAL
                     VKLSTWATILPGPGLIAFTAVVVLTMFASAAFDPKLIWERQ"
     gene            940..1599
                     /locus_tag="VIG70_04875"
     CDS             940..1599
                     /locus_tag="VIG70_04875"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY8249919.1"
                     /translation="MRYELFYWPSIQGRGEFVRLALEEGGADYRDVCRSSRRLPENPQ
                     AFAPPFLKAGKLIIPQTANILLYLGPRLGLAPKAEGERLRLHGLQLTIADWLAEVHDT
                     HHPIASSLYYEDQKREAKRRARIFLRERLPKFMRYFERAVDERFSYVHLSLFQMIEGL
                     RYAFPQGMAKLEKKHGRLVRLRDRVAARPRVAAYLASKRRIPFNQQGIFRHYPELDER
                     R"
     gene            1586..1900
                     /locus_tag="VIG70_04880"
     CDS             1586..1900
                     /locus_tag="VIG70_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249920.1"
                     /translation="MKDANYFLVVRFSVEPQAEAQLLRWLDGGHVAEVLRQKGFLWCK
                     RLRLGAHEFAMLYGIESRAAFEAYEANTALKAKFARERAPFEKHMRIERFCGEAETTY
                     AA"
     gene            1890..2267
                     /locus_tag="VIG70_04885"
     CDS             1890..2267
                     /locus_tag="VIG70_04885"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY8249921.1"
                     /translation="MPLDWNTGLVKHLGIRFVETEKDRIVAELDIRPELMTVGGALHG
                     GTLMAFADTIGAAGTVVNLNPGQSTATLESKTNFFAAAFEGVVRAESTPLHKGKRTHV
                     WQTRVTDANGKLLSLTIQTQVIL"
     gene            2272..3126
                     /locus_tag="VIG70_04890"
     CDS             2272..3126
                     /locus_tag="VIG70_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY8249922.1"
                     /translation="MGGIAAVLTAVMLSQTPFDLQGHRGARGHAPENTLPGFERALAI
                     GVDTLELDVGVTRDGVVVIHHDRRLNPDLARGPDGNWVAAPAPTIHSLSYPELQRYDV
                     GRIRPGSEYAQRFPHQKPIDGTPIPRLAALFSIVPSSIKFNIETKLVPEAPGETLPPE
                     PFARALIAEARKAGVAARTTIQSFDFRTLAVVQREAPEIATAYLTSGKTGEAVPRMVH
                     EAKGPIWSPNFQDLDPRALAVARELGVKVVAWTVNEPADIALVLDMKVDGIISDYPDR
                     VRAALKRR"
     gene            3123..3710
                     /locus_tag="VIG70_04895"
     CDS             3123..3710
                     /locus_tag="VIG70_04895"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY8249923.1"
                     /translation="MTPVTSALLVANIAVYLLGEMGVVSLGAFALWPPGGFESHFAPW
                     QLVTYAFLHGGFAHIFFNMLGLYMFGSEVERLFGSRFYLGYYFGCVVSAALCHLAVNA
                     WLGAAPYPTVGASGGVFGLLLAFGVYFPHRRVLLLFPPIPLPARVFVIGFAVLELVFG
                     ITGTAAGVAHFAHLGGMIGGWLMIQYRRGGFPFRS"
     gene            3784..4512
                     /locus_tag="VIG70_04900"
     CDS             3784..4512
                     /locus_tag="VIG70_04900"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEY8249924.1"
                     /translation="MADHAVQLYQEESDLADALTRFAAEGIALGEGVVVIGSTQRWQT
                     MTERLRAGGVDTHNAVLRGQLRLFGAKVILSSCMNRGVPDRFAFDAAIGGVLGLVRMR
                     YPVVRVFAELTDLLWAEGRRGAAEALERFWNKLAESQSFSLLCACPLDSLDGRAYEGA
                     LQSLCALHTHLVPAADCNAFNEAVGGAIREVLEPQLVGMLHSLSAQHRPATQMPIGQA
                     AILWLRQHMPRTAEKVLARARARM"
     gene            4645..5226
                     /locus_tag="VIG70_04905"
     CDS             4645..5226
                     /locus_tag="VIG70_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249925.1"
                     /translation="MPPAITVQRIAPQLVTFAGSAANFQNLVTGLSQGTQVQLFSTLP
                     DGFTQVVTFTPTAALAPEQIAQALETARQRLIGLGIANPTAEQIANTLLGVVVPTPLG
                     GSRIAGVLAPQNPPSPAVQIQANAAAGSTTPISTTPIPSIPAAATPVTSPVNVQLTAP
                     ATPAATPAPTPVQSSAKAAAQPGTANSGQRIAR"
     gene            5240..7483
                     /locus_tag="VIG70_04910"
     CDS             5240..7483
                     /locus_tag="VIG70_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY8249926.1"
                     /translation="MLYLNGRLRRHARVELVRKEKPAAREGPKAGPRSLLRLGYNCWA
                     IARAERAAFIVDAKDYFEAFYRAALLAKRSIMILGWDFNSQTRLHYDPVPKDGPPAVL
                     GDFLNYLTRRRRGLHIHVLTWDFPVMFAGDREVAPIFGLGWEPGRRVHFRYDDAHPVG
                     ASQHQKVAVIDDAMAFVGGIDLTLRRWDCCDHAPQDPRRTAEGQPYPPFHDTMIALDG
                     EAAHRLAEMTRERWRLATGQRLKPLEPDPQSDPWPAELEPHVTGVDVGIARTLPPRGE
                     APAVREVEKLYLDMIAAARRTIYIENQYFTAPRLAAALEKRLAEADGPEVVLVLRLLS
                     HGWLEEATMHVLRTRLIQKLQQADLHGRFRVYYPHVPGLAEGKCIDVHSKVMVIDDAI
                     LRIGSANLCNRSMALDTECDIVVESRGEAGVAAAIGDFRDRLLAEHLDTHPARVREET
                     ERAGSVNGAITVFRDQPRTLRQLDELPEWSEAVLNVAAVADPEEPIALETLLSERHAS
                     DAVSSDKASWGKLAGIVLAILALIALWRFTPLREAASAEAAIAWAKAFGEQPWAPWAL
                     ILSYTPACLVMFPRPLITLAAVLAFGPWLGFFYSLTGICASSAVTWYMGRHMRRDTVR
                     RLAGPRLDRMVDVLKKHGLLAMTLLRLVPLAPFAVESIVAGAIRMKLWHVVVGTAIGL
                     LPGTLTTTVFGDAIETAVSGSGEVNWWIVGGALTLLAAGAWAVKRWFTRMERRMQAHG
                     EAGGQPR"
     gene            7455..8234
                     /locus_tag="VIG70_04915"
     CDS             7455..8234
                     /locus_tag="VIG70_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY8249927.1"
                     /translation="MARPADSLANWRALDAQLGPDVLRVASYNVHSCCGADGKKDAQR
                     IARVIDECGVDTVGLQEADHRLDYIAQQLGMQAIPGLTLARHDGPFGNALLTRRKVLA
                     VRRLGFTYSRREPRNALDVDLEVAGETVRVIVTHLGLWPAERRFQVKQILKMLRETPI
                     CERVVVLGDINEWLPLGRPLRWMHALFGRSPAERSFPSRWPMVALDRVWVRPRHALLA
                     LKAHRSALAQQASDHLPVKAIVAIHQAENRAEQRNCEVLAA"
     gene            complement(8307..8723)
                     /locus_tag="VIG70_04920"
     CDS             complement(8307..8723)
                     /locus_tag="VIG70_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249928.1"
                     /translation="MGPASTTALGAIVGLIVFLAMALTGVIAVNGVHNPGIGIAPAHA
                     SAAAGSTGEASAERPPSMRSLDLNGDGKLSLAEAAGYGEIVTRFDRADRNRDGKLTQA
                     EFERLAKLPPPKAGKAKPRVVRRPATTEERSAAAGG"
     gene            8813..9880
                     /locus_tag="VIG70_04925"
     CDS             8813..9880
                     /locus_tag="VIG70_04925"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEY8249929.1"
                     /translation="MKLAIALLAGLLFLGNAHAQRVYDQAELDAMLAPIALQPDGVVS
                     QVLIAATYPDEVHAAARWSRANPHMRGDPALRAVENEPWDPAVKALVAFPELLTRMVE
                     SPQWLQDLGEAFVRQQAQVMDTVQALRRRAQAAGHLASTDQTAVYDQGEAIVVQPRTQ
                     IVYVNYYDPYVVYGPWWWPYYRPVFWHPWVVRPVFVAHGFFYSKPVWQHRHVAVVHPP
                     VHVVRHSVVPGKWQHGKYATGVVKPHVVSKPYVRVPESQRKPIVQQHMPAAGVVKPHV
                     GSKPVVSKPYVRVPESQRQPIVQNHMPAASGFTQHYAPRQQHAEVRHGPQVGHAAGGH
                     GPRGGGQGQGGGRSQGGYGRR"
     gene            9925..10791
                     /locus_tag="VIG70_04930"
     CDS             9925..10791
                     /locus_tag="VIG70_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEY8249930.1"
                     /translation="MIIRKQEDVTEAVLAEMHRTPDARTRQILGSLVTHLHAFVREVR
                     LTDKEFHEAIGHINAIGQKTTPSHNEAMLVAGALGVSNLVCLLNNAHPTQANNLGPFY
                     RAGAPHCADGDSLLRSPTPGERLVFKGFVKDTRGKPVAGAEVDVWHSSPVGLYENQDP
                     GQAEMNLRGRFTTLADGSFSFTTVKPAGYPVPIDGPNGALLGAQKRHNFRPAHLHFLI
                     YKPGFKTIASQVYDPADPNLQTDSQFGVTDALIGNFVRKDDGYLLEFTFVIAPGEARL
                     PRAPITEKAKAL"
     gene            10800..11858
                     /locus_tag="VIG70_04935"
     CDS             10800..11858
                     /locus_tag="VIG70_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HEY8249931.1"
                     /translation="MAWNAGVPLAACALLAATWSRPLGWLLVCLVAAALVAVVLVAVH
                     HAEVVALRVGEPFGTLILALAVTVIEAGLIVSMMLSGGAGTSALVRDTVFATVMIICN
                     GVVGLCLLTGAIRHHVLAFRIEGTNQALSVLASLVTLTLVLPAYTTTVPGPIYSPGQL
                     VFAGAASLILYAMFVFVQTVRHRGYFLPIGAEGDEEAPPPSARAAWASLGLLALSLVA
                     VIGLAKTLSPSIQSGVEAAAAPAAVVGIAIALLVLAPETVAAVRAANRNRMQTSLNLA
                     LGSALATIGLTIPTVAALSILLGLPLELGLAAKETVLLALTLLVSTLTIASGSATVLQ
                     GVVHLVLFAAFLFLAVVP"
     gene            complement(11866..12735)
                     /locus_tag="VIG70_04940"
     CDS             complement(11866..12735)
                     /locus_tag="VIG70_04940"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249932.1"
                     /translation="MLTGYPAGGLVDVVARLVAEQMKPKFPRGLVVQIKPGAAGSVAV
                     TELVRSQPDGYTFILTPHSALVIAPQLQELAYKTPEDYQPFINVVSYYPMIAVRSESP
                     YQNINQLVADAKAKPGQIRVGSPGEGTSSHLNLEEFMRLAGIKMIHAPYQGWAHSSVA
                     VLGGHIDAVVAQPGELKGQVDGKRMRILVNFTPQRHPVFPDVPTARELGWDVSNGVWY
                     LLMVPKGTPAGVVKYLHDAAKDAVEDPRFGKIMAERGIDVDYRPADKLRADLAREYKA
                     HTEILRRIGMIKN"
     gene            complement(12854..14650)
                     /locus_tag="VIG70_04945"
     CDS             complement(12854..14650)
                     /locus_tag="VIG70_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY8249933.1"
                     /translation="MRIEDYALIGDCHTAALVGLNGSIDWLCWPRFDSAACFAALLGG
                     PENGYWKIAPVGVSKASRSYRADTLILETEFETAEGAVTLVDFMPVRSSQSDLVRMVI
                     GRRGRVAMQSVGVLRFDYGASVPWVTLSADRKALRAVAGPDLALLCSPVPLENRERKT
                     RARFEVSAGETLSFVLTHTASHLDVPRQVDAGKALDDTEAFWKHWSARCTVGGEWGGT
                     VRRSLITLKALTYAPTGGIVAAPTTSLPERIGGVRNWDYRYCWLRDATLTLLALMDGG
                     YYDEARAWREWLVRAVAGSPEQMQIMYGIAGERRLPEFEVPWLAGYEKSKPVRIGNAA
                     AAQLQLDVYGELIDALYQACKGGLAGHDIAWSLQRALLDHLEKVWTEPDAGLWEIRAS
                     PRHFTYSKVMAWVAFDRAIKMVEGFGVAGPVERWRELRARIHADVCERGFSRKRNAFV
                     QSYGSEELDASLLLIPITGFLPVEDSRVTATVEAIRRDLTVDGLVLRYRTHSAADGLP
                     QGEGVFLACSFWLADNLCMQARWDEAHDLFARLTGLANDVGLLAEEYDPVARRFLGNF
                     PQAFSHVGLVNTAMNLFERDKPAEQRAEKKAA"
     gene            complement(14683..15702)
                     /locus_tag="VIG70_04950"
     CDS             complement(14683..15702)
                     /locus_tag="VIG70_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8249934.1"
                     /translation="MTAEPRAIAARGDLASDEKSTIELFERSKGSVVFITTTQLVRDR
                     WSRNMFSIPRGAGSGFVWDADGHVITNNHVVSGAAEAKVRLNDGRDYSARLVGASPAH
                     DIAVLRIRVPERQPTALPIGTSADLKVGQKVFAIGNPFGLDWSLTTGVVSALDRSLPA
                     EDERTVIEHLIQTDAAINPGNSGGPLLDSSGRLIGINTAIFSPSGAYAGVGFAVPVDT
                     VNRVVPQLIAKGKYTRPVLGISTDEQLNQLIAKQLGVKGVAVLKVEPGSPAEAAGLRA
                     ARSGADGSLVPGDVITALDGKAVDSVARLVNRLDEHKVGDTIRLSVQREGKAAELSVT
                     LKAGT"
     gene            15880..17301
                     /locus_tag="VIG70_04955"
     CDS             15880..17301
                     /locus_tag="VIG70_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY8249935.1"
                     /translation="MRDYVDIAFGLAVLVAALFASGHAVLYKRESRSASLWVILIWVM
                     PVLGPILYMLMGVNRVQRRAARMRAFMVRHRADPQTIQGEPPGTHLAPLARMLNNVVE
                     RPLLPGNSVEVLVDGTQAYPAMLEAIESARASVMLASYIFDSDGIGAEFVAALERAHK
                     RGVAVRVLVDDVDVRFSRRPVTKPLRRAGVPVGVFNPPFVPARLNAVHLRNHRKILVV
                     DGTVGFTGGMNIDGRYWSADAAGEGKEISRDLHFRLQGPVLAQLAEVFADDWQFVTGE
                     ALRGAPWFVPLEPAGSVLARCIDDGPDESTEPLRWAIVAGLNQAQRSVRIMTPYFVPD
                     GALITALDVAAMRGVDVDIVLPARSDLPHVHWAAWGQLWQVLERGCHVWCTPGPFDHS
                     KLMVVDGAWTLLGSANWDARSLRLNFELNVECYSVEFGAHMEGLVLARINTAKPVTLS
                     DINARSLPVKLRDGVARLFAPFL"
     gene            17381..18133
                     /locus_tag="VIG70_04960"
     CDS             17381..18133
                     /locus_tag="VIG70_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8249936.1"
                     /translation="MPRGPRLVVPGVALHVVQRGHDRRDCFQHETDYLVYLSSLRELS
                     TQTQCALHAYCLMTNHVHLLLAPSSVQGCAVLMRNLGQRYVQYFNRRYQRRGTLWEGR
                     YHSCLVDSAPYVIACYRYIERNPVRAGMVASAADYLWSSHKGNAGEANNKLLTPHPEY
                     SALALNDASRHTAYQGLFAAADDPAFLAAIRDATNGGFALVGEALKASLPANLQHRLE
                     RKQPGPRPAPTAQSVDLAAQMTLELGLRPRTS"
     gene            complement(18149..>19698)
                     /gene="ettA"
                     /locus_tag="VIG70_04965"
     CDS             complement(18149..>19698)
                     /gene="ettA"
                     /locus_tag="VIG70_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY8249937.1"
                     /translation="GVLGLNGSGKSSLLKIMAGTDDNYEGEVVRMPGIRVGYLEQEPV
                     LGKNKTVREAVEESLGETAAAKKRLDEVYAAYSEPDADFDKLAEEQAKLEAMINSGGA
                     DELEIAADALRLPPWEQKVDVLSGGEKRRVALCRLLLSKPDLLLLDEPTNHLDAESVE
                     WLEQFLQRFPGTVVAVTHDRYFLDNAAGWILELDRGYGIPWEGNYSSWLEQKEKRLQV
                     EASQEVARIKAMQAELEWVRQNPKGRGTKSKARLARFEELSSQETQTRNETQEIFIPV
                     AERLGNEVIEFQNVSKGYGERLLIDKLSFKVPPGAIVGIIGPNGAGKSTLFKLINKKE
                     KPDSGKILIGKTVKIAHADQSREELQSEKTVFEDISGGQDMLKLGRFEMPARSYLARF
                     NFRGADQQKPVGQLSGGERGRLHLARMLMSGANVLLLDEPSNDLDVETLRALEEALLE
                     FAGCVLVISHDRWFLDRIATHILAAEDDSQWVFFNGNYQEYEADKRKRLGEEAAKPHR
                     IKYKALK"
BASE COUNT         3061 a         6864 c         6508 g         3265 t
ORIGIN      
        1 cgccgccgag tcgtgcaggc gcgccttcac caccgcctgc ttgcggtcgt cggacagctt
       61 gatggccgtc acttcgccga tctgcacgcc gcgatagatg accggcgtct ggccgacgcg
      121 caggccggcg gcgtcgtcga aacggatcgc gatctcgggg ccgagcgaac gcatgtgctg
      181 ccatacgagg tacaccccca ccgcgagcgc gatgacgggc accacccaga ccagccggaa
      241 caggcgccgg cggcggatcc tcgccttggg aagctcttcg ccggtcattg ccgctcccag
      301 atgagcttcg gatcgaacgc cgccgaggca aacatggtca gcacgaccac ggcggtgaag
      361 gcgatcaggc ccgggccggg caggatcgtc gcccacgtgc tgagcttgac cagcgccacc
      421 agcacggcga gcaggaacac gtccagcatc gcccacgggc cgatcgcgtc gatgaacttg
      481 tagagctggg tgcggctgcg caggcgccgg ccccagccca tccaagccgt caccacgagg
      541 gagaagagcc cggccagctt caccaccgga atcaccatgc tcgcaagaaa gacgatcacc
      601 gcgacgaccc actggtcggt acgcacgagc gtgacgacgc cgtcccacac cgtgctctcc
      661 gagtacccgc cgtagaggta catcctcatg atcggataga cgttcgccgg cacgtagagg
      721 atcaacgcgg caatcgagag cgccgcggtg acttcgaggc tgcgggtgga gctgcgcgcc
      781 gcgacgaacg agccgcaacg gatgcactcg gccgcggtgc ctgggacgag gccttccacc
      841 gtctgcacca ggccgcaggt atcgcaggaa acgaccgact cttgggccat cgaaatggcc
      901 gtgcaatcag catgcccggc ctcatgtacc cttcggagca tgcgctatga gctgttctac
      961 tggccctcga tccagggtcg cggagagttc gtgcgactgg cgctggagga aggcggggcc
     1021 gattaccggg atgtctgccg ctcctccagg cgcctccctg agaaccccca agcgttcgcg
     1081 ccgccgttcc tcaaggcggg aaagctgatc atcccgcaga ccgccaacat cctgctctat
     1141 ctcggcccgc gcctcggcct ggcgccgaaa gccgagggag agcgcctcag gctgcacggg
     1201 ctacagctca ccatcgccga ctggctcgcc gaggtgcacg acacccacca tccgatcgcc
     1261 tccagcctct actacgagga ccagaagcgc gaggcgaagc ggcgcgcgcg gatttttcta
     1321 agggagcgtc tgccgaagtt catgcgctat ttcgagcgcg ctgtggacga gaggttctcc
     1381 tacgtgcatc tgtcgctctt ccagatgatc gaaggactgc gctacgcttt tccgcaaggg
     1441 atggcgaagc tggagaaaaa gcatggcaga ttggtcaggc tgcgtgatcg cgtcgcggca
     1501 cggccgcgcg tcgccgccta cctggcgtcg aagcgccgca tccccttcaa ccagcaaggc
     1561 atcttccgcc actatccgga gctcgatgaa agacgctaac tactttctgg ttgtccgctt
     1621 ctcggtcgag ccgcaggccg aagcgcagct gctgcgctgg ctcgacggcg gtcacgtcgc
     1681 cgaagtcctg cggcagaagg gcttcctctg gtgcaagcgc ctgcggctgg gcgcgcacga
     1741 attcgccatg ctctatggca tcgaatcgcg cgcagcgttc gaggcctacg aagcgaacac
     1801 ggcgctcaag gcgaaattcg cccgcgagcg cgcaccgttc gagaagcaca tgcgcatcga
     1861 gcgcttttgc ggagaggcgg agaccactta tgccgcttga ttggaacacc ggcctggtaa
     1921 agcacctcgg catccgcttc gtcgaaacgg agaaagaccg catcgtcgcc gagctcgaca
     1981 tccggccgga gctcatgacg gtgggcggcg cgctgcacgg cggcactctg atggcgttcg
     2041 ccgacacgat cggcgcggcc ggcacggtgg tgaacctcaa tcccgggcag agcacggcga
     2101 cgctcgagtc gaagaccaac ttcttcgccg ccgcgttcga gggggtggtg cgcgcggagt
     2161 cgacgccgct gcacaagggc aagcgcacgc acgtgtggca gacgcgcgtg accgatgcca
     2221 acggcaagct gctgtcgctt accattcaga cccaggtgat cctctaggcc gatgggcggg
     2281 atcgccgcgg tcctgaccgc cgtcatgctg agccagacac ctttcgactt gcagggccac
     2341 cgcggggcac gcgggcacgc gcccgagaac acgctgccgg ggttcgagcg cgcgctcgcc
     2401 atcggcgtcg acacgctcga gctcgacgtc ggcgtcacgc gcgacggcgt ggtggtgatc
     2461 catcacgacc gccggctcaa ccccgacctc gcgcgcggcc ccgacggcaa ctgggtcgcc
     2521 gcgcctgcgc cgacgattca ttcattgagc tacccggagc tgcagcgcta cgacgtgggg
     2581 cgcatccggc cggggtcgga atacgcgcag cgctttccgc accagaagcc gatcgatggc
     2641 accccgattc cgcgcctggc cgcgctcttt tccatcgtcc cttcttccat caagttcaac
     2701 atcgaaacca agctggttcc cgaggcgccc ggagaaaccc tccctccgga gcctttcgcg
     2761 cgcgcgctca tcgccgaggc gcgcaaggcc ggcgtggcgg cgcgcacgac gatccagtcc
     2821 ttcgacttcc gcaccctggc ggtcgtgcag cgcgaggcgc ccgagatcgc cacggcctat
     2881 ctcacctccg ggaaaacggg cgaggccgtg ccgaggatgg tgcacgaagc gaaaggcccc
     2941 atctggtcgc cgaacttcca ggacctggat ccgcgcgcgc tggcggtggc gcgcgaattg
     3001 ggcgtcaagg tggtcgcgtg gacggtcaac gagcctgcgg acattgcgct cgtcctcgac
     3061 atgaaggtgg atggcatcat cagcgattac cccgatcgcg tgcgcgcggc gctgaaacgg
     3121 cgatgacccc ggtcacgagc gcgctgctgg ttgcgaatat cgccgtgtac ctgctgggcg
     3181 agatgggcgt ggtctcgctc ggcgcgttcg cgctctggcc gcccggcggg ttcgagtcgc
     3241 acttcgcgcc gtggcagctc gttacctatg ccttcctgca tggcggcttc gcgcacattt
     3301 tcttcaacat gctcggcctg tacatgttcg gctccgaggt ggagcgcctg ttcggctcgc
     3361 gtttctacct cgggtactac ttcggctgcg tggtgagcgc ggcgctctgc cacctcgcgg
     3421 tcaacgcctg gctgggcgcc gctccctacc ccactgtcgg cgcctcgggc ggcgttttcg
     3481 gcctgctgct cgcgttcggc gtctacttcc cgcaccgccg cgtgctcctt cttttcccgc
     3541 cgattccgct cccggcgcgc gttttcgtga tcgggttcgc ggtgctcgag ctggtgttcg
     3601 gcattaccgg caccgcagcg ggcgtggcgc acttcgcgca cctcggcggc atgatcggcg
     3661 gctggctgat gatccagtac cgccgcggcg gcttcccctt ccgcagctag tgtcgatgcg
     3721 gtgctacagc gggttacggc ggcggcgaca gccaaacctg ccgcacaggc ctatcgtggc
     3781 tggatggcag accacgccgt ccagctctac caggaggaaa gcgatctcgc ggacgcgctg
     3841 acgcggttcg cggccgaggg catcgcgctc ggcgagggcg tggtggtgat cggcagcaca
     3901 cagcgctggc agacgatgac cgagcgcctg cgcgcgggcg gcgtcgatac gcacaacgcg
     3961 gtgctccgcg ggcagcttcg cctcttcggc gccaaggtga tcctgtcgag ctgcatgaac
     4021 cgcggcgtgc ccgaccgctt tgcgttcgat gcggcgatcg gcggcgtgct ggggctcgtg
     4081 cgcatgcgct acccggtcgt gcgcgtgttc gccgagctga ccgacctcct ctgggccgag
     4141 ggacggcgcg gcgccgccga ggcgctcgag cgcttctgga acaagctcgc cgagtcgcaa
     4201 tccttttccc tcctgtgcgc ttgcccgctc gacagcctcg acggccgcgc ctacgaaggc
     4261 gcgctacaga gcctctgcgc gctgcatacc catctcgtgc ccgcagccga ctgcaacgcc
     4321 ttcaacgaag ccgtcggcgg cgccatccgc gaggtgctcg agcctcagct cgtcggcatg
     4381 cttcattcgc tatccgccca gcaccgtccc gccacccaga tgccgatagg acaggcggcc
     4441 atcctgtggc tgcggcagca catgccgcgc acggcggaga aggtcctcgc ccgcgcccgg
     4501 gcgaggatgt agtggggcac gaaaagtgca tgagccgtcc cgaaaggagg cttcatgcca
     4561 ttacccgccc gttttgccgc atttctccct cttgtagccg cgctagccat agcctgtcag
     4621 ggcgcagcgg cccagaccat cgtcgtgccg cccgccatca cggtgcagcg catcgcgccg
     4681 cagctcgtca cctttgcggg ctcggcggcc aacttccaga acctggtgac cggcctctcc
     4741 cagggaactc aggtgcaact tttttcgacg ttgcccgacg gctttaccca ggtcgtcacg
     4801 ttcactccca ctgcggccct cgcgcccgag cagatcgcgc aggcgctcga gaccgcgcgc
     4861 cagcggctga tcgggctcgg gatcgcgaat cccaccgcag agcagatcgc gaacacgctg
     4921 ctgggtgtgg tggtacccac gcccctcggc ggctcgcgga tcgcgggcgt gctggccccg
     4981 cagaatccgc cgagtccggc ggtgcagatc caagccaatg ccgccgccgg aagcaccacg
     5041 ccgatcagca ccacgccgat cccgtcgata ccggctgccg caacgccggt gacgtcgccg
     5101 gtgaacgtgc agctcacggc gccggccacc ccggccgcaa cgccggcgcc cacgcccgtc
     5161 cagtcgtcgg cgaaagcggc ggcgcagccc ggaactgcga actcaggcca gagaatcgcg
     5221 agataagcgc gtgggcagcg tgctctacct aaacggtagg ctgcgccgcc acgcgcgcgt
     5281 cgagctcgtc cggaaggaaa agccggccgc gcgcgagggg cccaaagccg ggcctcgctc
     5341 gctcctgcgc ctggggtaca actgctgggc gatcgcgcgc gccgagcgtg ccgccttcat
     5401 cgtcgacgcg aaggactact tcgaggcctt ctatcgcgcc gcgctcctcg ccaagcgctc
     5461 gatcatgatc ctcggctggg atttcaacag ccagacgcgg ctgcattacg acccggtgcc
     5521 gaaggacggc ccgccggcgg tgctcggcga cttcctcaac tacctcaccc ggcgccggcg
     5581 cggcctccac atccacgtcc tgacgtggga cttcccggtg atgttcgccg gcgaccgcga
     5641 ggtggcgccg atcttcggcc tcggctggga acccgggcgg cgcgtgcatt tccgctacga
     5701 cgacgcgcat cccgtcggcg cgagccagca tcagaaggtg gcggtgatcg acgatgcgat
     5761 ggcctttgtc ggcggcatcg acctgacctt gcggcgctgg gactgctgcg accacgcgcc
     5821 ccaggacccg cgccgcaccg ccgagggtca gccgtacccg ccgttccacg acaccatgat
     5881 cgcgctcgac ggcgaggcgg cgcatcggct cgccgagatg acgcgcgagc gctggcggct
     5941 cgccaccggc cagcgcctga agccgctcga gccggacccg caatccgatc cctggcccgc
     6001 cgagctcgag ccgcacgtca ccggcgtcga cgtgggcatc gcccgcaccc tgccgccgcg
     6061 cggcgaggct ccggcggtgc gcgaggtgga gaagctctac ctcgacatga tcgccgcggc
     6121 gcggcgcacg atctacatcg agaaccagta cttcaccgcg ccgcggctcg ccgccgcgct
     6181 cgagaagcga ctggccgagg ccgacgggcc ggaagtcgtc ctggtgctgc ggctgctctc
     6241 gcacggctgg ctcgaggaag cgacgatgca cgtcctgcgt acgcgcctca tccagaagct
     6301 gcagcaggcc gacctccatg gccgcttccg cgtgtactac ccgcacgtcc cggggctggc
     6361 cgagggcaag tgcatcgacg tgcattcgaa ggtcatggtg atcgacgacg cgatcctgcg
     6421 catcgggtcg gcgaacctgt gcaaccgctc gatggcgctc gacaccgagt gcgacatcgt
     6481 ggtcgagtcg cgcggcgagg cgggcgtcgc cgcggcgatc ggcgatttcc gcgatcgcct
     6541 gctcgccgag cacctcgata cccaccccgc gcgagtgcgc gaggaaaccg agcgcgccgg
     6601 cagcgtcaac ggggcgatca ccgtgttccg cgaccagccg cgcaccttgc gccagctcga
     6661 cgagctgccg gagtggtccg aggcggtact caacgtcgcg gcggtggccg atcccgagga
     6721 gccgatcgcg ctcgagacgc tcctctccga gcgccacgcc agcgacgcgg tctcgagcga
     6781 caaggcctcc tggggcaagc tcgccggcat cgtcctcgcg atcctcgcgt tgatagcgct
     6841 ctggcgcttc acgccgctca gggaagccgc gagcgccgag gccgccatcg cctgggccaa
     6901 ggcgttcggc gagcagccgt gggcgccctg ggcgctgata ctgtcctaca cgccggcctg
     6961 cctcgtgatg ttcccgcgcc cgctgattac cctcgccgcg gtcctcgcat tcggcccgtg
     7021 gctcggcttc ttctacagcc tgaccggcat ctgcgcctcg tcggccgtga cctggtacat
     7081 ggggcgccac atgcggcgcg acacggtccg ccgcctcgcc gggccgaggc tcgaccgcat
     7141 ggtcgacgtg ctgaaaaagc acgggctgct ggcgatgacg ctgctgcgcc tggtgccgct
     7201 cgcgccgttc gcggtggaga gcatcgtcgc cggcgcaatc cgcatgaagc tctggcatgt
     7261 cgtcgtgggc accgcgatcg gcctgctgcc gggcaccctg accaccacgg tcttcggcga
     7321 cgcgatcgag accgcggtca gcggctcggg cgaagtgaac tggtggatcg tcggcggcgc
     7381 gctgacgctg ctggccgccg gcgcctgggc cgtgaagcgc tggttcaccc gcatggaacg
     7441 cagaatgcaa gcgcatggcg aggccggcgg acagcctcgc taactggcgc gcgctcgacg
     7501 cgcagctcgg cccggatgtc ctgagggtgg cctcctacaa cgtccacagc tgctgcggcg
     7561 ccgatggcaa gaaggacgcc cagcgcatcg cgcgcgtgat cgacgagtgc ggcgtcgaca
     7621 ctgtcggcct gcaggaggcc gaccaccggc tcgactacat cgcgcagcaa ctcggcatgc
     7681 aggcgatccc cggcctgacg ctcgcgcgcc acgacggccc attcggcaac gcgctgctca
     7741 cccggcgcaa ggttctggcg gtgcggcggc tgggcttcac ttactcgcgg cgcgagccgc
     7801 gaaacgccct cgacgtcgac ctcgaagttg ccggggagac tgtgcgcgtg atcgtcacgc
     7861 atctcgggct gtggccggcg gagcgccgtt tccaggtgaa acagattctg aaaatgctga
     7921 gagaaacccc gatttgtgag cgcgtggtgg ttctcggcga catcaacgag tggctgccgc
     7981 tcgggcggcc gctgcgctgg atgcacgcgc tcttcgggcg ctcgcctgcc gagcgctcgt
     8041 ttccctcgcg ctggccgatg gtcgcgctcg accgcgtgtg ggtgcgcccg cgccatgccc
     8101 tgctcgcgct caaagcgcac cgcagcgcgc tcgcccagca ggcctcggac catctccccg
     8161 tgaaggcgat cgtcgcgatc catcaagcgg aaaaccgggc tgaacagcgg aactgcgaag
     8221 tgctagcagc ttgaattcgg ggtcagggtg cgaattctgt tttcacgtac gtaaaaacga
     8281 attcgcaccc tgaccccgaa ttcggtctag ccgccagcgg cggccgatct ctcttccgtc
     8341 gtcgccgggc gacgcaccac tctgggcttg gcctttccgg ccttgggcgg cgggagcttg
     8401 gcgagccgtt cgaactccgc ctgggtgagc ttcccgtccc gattgcggtc ggcgcggtcg
     8461 aagcgcgtga cgatctcgcc atagccggcg gcttcggcga gcgacagctt gccgtcgccg
     8521 ttcaggtcga gcgagcgcat cgagggcggt ctttcggcgc tcgcctcgcc agtgctgccg
     8581 gcagccgccg acgcatgtgc cggcgcgatc ccgatccccg ggttatggac gccattgacc
     8641 gcgatcaccc cggtcagggc catcgccagg aagacgatga gcccgacgat ggcgcccaag
     8701 gcggtggtac tggcgggtcc catgccccct ccgccgcagg atgcgtgccc ggggttactt
     8761 ctggttacac atcaggacag atgctgcgct gctcaacgtc tacattagcc tcgtgaaact
     8821 cgcgatcgcg ctcctcgccg gcctcctctt cctgggtaac gcccacgcgc agcgcgttta
     8881 cgaccaggcc gagctcgacg cgatgctcgc cccgatcgcg cttcagcccg acggggtcgt
     8941 ttcccaggtg ctgatcgccg cgacctatcc cgacgaagtc cacgctgccg cgcgctggtc
     9001 gcgcgcgaat ccgcacatgc gtggcgaccc ggcgttgcgc gcagtggaga acgagccgtg
     9061 ggacccggcg gtcaaggcgc tggtcgcttt ccccgagctg ctgacccgca tggtcgaaag
     9121 cccgcagtgg ctgcaggacc tcggcgaggc cttcgtcagg cagcaagcgc aggtcatgga
     9181 cacggtgcag gcgctgcgcc gccgcgcgca agccgccggc caccttgcga gcacggacca
     9241 aaccgcggtc tacgaccaag gcgaagcgat cgtggtgcag ccgcgcaccc agatcgtcta
     9301 cgtcaactat tacgacccct acgtcgtcta tgggccgtgg tggtggccgt attaccgccc
     9361 cgtgttctgg cacccatggg tcgtgcgccc ggtcttcgtc gcgcacggct tcttctacag
     9421 caagcccgtc tggcaacacc gtcatgtcgc cgtggtgcac ccgccggtcc acgtcgtccg
     9481 ccacagcgtg gttcccggca aatggcagca cggcaagtac gcaacgggtg tggtgaaacc
     9541 ccacgtcgtg tcgaagccct acgttcgcgt gcccgagtcg cagcgcaagc cgatcgtgca
     9601 acagcacatg ccggcggccg gtgtggtgaa gccccacgtc gggtcgaagc ccgtggtgtc
     9661 gaaaccctat gtccgcgtgc ccgaatcgca gcgccagccg atcgtgcaga accacatgcc
     9721 ggcggcgagc ggattcacgc agcactatgc gccgcggcag caacacgccg aagttcgcca
     9781 cgggccccag gtcggacacg ccgcgggtgg acacggcccg cgcggcggcg gccaaggtca
     9841 gggcggcggt cgtagccagg gcggttacgg ccgccgctag cagcacgtga gctagacttt
     9901 cctcctgcgg gaggagagcg gctcatgatc attcgcaagc aggaggacgt caccgaagcg
     9961 gtgctcgcgg agatgcaccg cacccccgac gcgcgcacga ggcagattct cgggtctctg
    10021 gtgacgcact tgcacgcctt cgtgcgcgag gtgcggctga cggataagga attccacgag
    10081 gcgatcggcc atatcaacgc gatcggccag aagaccacgc cgagccacaa cgaggccatg
    10141 ctggtggccg gcgcgctcgg ggtgtcgaac ctcgtctgcc tgctgaacaa cgcccacccg
    10201 acgcaggcga acaacctggg gcccttctac cgcgcgggcg cgccgcactg cgccgatggc
    10261 gattcgctgc tgcgctcgcc gacgccgggc gaaaggctgg tgttcaaggg gttcgtcaaa
    10321 gacacccgcg gcaagccggt cgcgggcgcc gaggtcgacg tctggcactc ctccccggtg
    10381 ggcctctacg agaaccagga ccccggccag gccgagatga acctgcgcgg caggttcacc
    10441 acgctcgccg acggctcgtt cagcttcacc acggtcaagc ccgccggcta cccggtgccg
    10501 atcgacggac cgaacggggc actgctcggg gcgcagaagc gccacaactt ccgtcccgcg
    10561 cacctgcatt tcctcatcta caagcccggc ttcaagacca tcgcatcgca ggtctacgat
    10621 cccgcggacc cgaatctcca gaccgacagc cagttcggcg tgaccgacgc cctcatcgga
    10681 aacttcgtcc gcaaggacga tggctatttg ctcgagttca cgttcgtcat cgcgccgggc
    10741 gaggcacggc tgcccagggc gccgatcacg gagaaggcga aagcgctgta gtgaaggcga
    10801 tggcgtggaa cgccggggtg ccgctcgccg cttgcgcgct gctcgcggcg acgtggtcgc
    10861 ggccgctcgg ctggctgctc gtctgcctgg tcgcggcagc gctggtcgcg gtggtgctgg
    10921 tcgcggtcca tcatgccgag gtcgtcgcgc tgcgcgtcgg cgagcccttc ggcacgctga
    10981 tcctcgcgct ggcggtcacg gtgatcgaag ccggcctgat cgtctcgatg atgctttccg
    11041 gcggcgcggg caccagcgct ctcgtgcgcg ataccgtgtt cgccacggtg atgatcatct
    11101 gcaacggcgt ggtcggactg tgcctgctga ccggcgccat ccgccaccac gtgctcgcat
    11161 tccgcatcga gggcacgaac caggccctct cggtgctcgc gtcgctcgtc acgctgacgc
    11221 tcgtgcttcc cgcttatacg accaccgtgc cgggcccgat ctactcgccg ggccagctcg
    11281 tgtttgccgg cgctgcgtcg ctcatcctgt acgcgatgtt cgtcttcgtg cagacggtgc
    11341 ggcatcgcgg ctatttcctg ccgatcgggg ccgaggggga cgaggaagcg cccccgccat
    11401 ccgcgcgcgc ggcgtgggcg agcctgggat tgctggcgct ttccctggtg gcggtgatcg
    11461 gactcgccaa gacgctctct ccgagcatcc agtcgggcgt cgaagccgcg gcagcgcccg
    11521 cggcggtcgt cggaatcgcc atcgcgctcc tggtgttggc gccggagacc gtggcggcgg
    11581 tgcgagcggc gaaccgcaac cggatgcaaa cgagcctcaa cctcgccctc gggtcggcgc
    11641 tcgccacgat cggcctgact atcccgacgg tggcggcgct gtcgatcctg ctcgggctgc
    11701 cgctggagct cgggctggcc gccaaggaaa ccgtcctgct cgcgctcacc ctgctcgtct
    11761 caaccctgac gatcgccagc ggcagcgcga ccgtgctgca aggcgtcgtg cacctcgtcc
    11821 ttttcgccgc gttcctcttc ctggcggtgg tgccttgaaa agcgcctagt tcttgatcat
    11881 gccgatgcgg cgcaggatct cggtgtgagc cttgtactcg cgcgccaggt ccgcgcgcag
    11941 cttgtcggca gggcgatagt ccacgtcgat gccgcgctcg gccatgatct tgccgaaccg
    12001 cgggtcttcg accgcgtcct tcgcggcgtc gtgcaggtac ttgaccacgc cggccggcgt
    12061 gcccttcggc accatcagga ggtaccagac gccgttcgac acatcccagc cgagctccct
    12121 cgccgtcggc acgtccggga acacgggatg gcgctgcggc gtgaaattga ccagtatgcg
    12181 catgcgcttg ccgtcgacct ggcccttgag ctcgcccggc tgcgcgacca cggcgtcgat
    12241 gtgcccgccc agcaccgcga cgctcgaatg cgcccagccc tggtagggcg cgtggatcat
    12301 cttgatgccg gcgaggcgca tgaactcctc gaggttgaga tggctcgagg tgccctcgcc
    12361 cggggagccg acgcggatct ggcccggctt ggctttcgca tccgcgacca gctgattgat
    12421 gttctgataa ggggactcgg aacgcaccgc gatcatgggg taataggaca ccacgttgat
    12481 gaacggctga tagtcctccg gcgtcttgta ggcgagctcc tggagttgcg gcgcaatgac
    12541 cagcgccgaa tgcggcgtga ggatgaaggt gtagccgtcg ggctgcgagc gcaccagctc
    12601 ggtgaccgcg accgagcccg ccgccccggg cttgatctgc accaccaggc ccctggggaa
    12661 tttcggcttc atctgctcgg cgaccaggcg cgccaccaca tcgaccaggc cgcccgccgg
    12721 ataaccggtg agcatcgtga ccggacgctc cgggtattgt gctgcagcgg gaaaggacag
    12781 cgatgcagct acagcaagcg cacacccaaa aatggttttc agcatgacga aagcccctcc
    12841 tcggggatcg attctaggcg gctttcttct cggcgcgctg ctcggcgggc ttgtcgcgct
    12901 cgaagagatt catcgcggtg ttgacgaggc cgacgtgcga gaacgcctgc ggaaagttgc
    12961 cgaggaagcg gcgcgcgacc gggtcgtact cctcggcgag caggccgacg tcgttggcga
    13021 ggccggtcag gcgcgcgaac aggtcgtgcg cctcgtccca gcgggcctgc atgcacaggt
    13081 tgtcggcgag ccagaagctg caggcgagga acacgccttc gccctgcggc aggccgtccg
    13141 cggccgagtg cgtgcgatag cgcagcacca ggccgtccac ggtcaggtcg cggcgaatgg
    13201 cttccacggt agcggtgacg cgtgaatcct caaccgggag aaagccagtg atggggatca
    13261 ggagcaggct cgcgtcgagc tcctccgacc cgtagctctg cacgaaggcg ttgcgcttgc
    13321 ggctgaaccc gcgctcgcag acgtcggcgt ggatccgcgc gcgcagctcg cgccagcgct
    13381 cgaccggtcc ggcgacgccg aagccttcga ccatcttgat cgcgcggtcg aatgcgaccc
    13441 acgccatcac tttcgagtag gtgaagtgcc gcggggacgc gcggatttcc cacaggcccg
    13501 cgtcgggctc ggtccaaacc ttttccaggt ggtcgagcag cgcgcgctgc aggctccagg
    13561 cgatgtcgtg cccggcgagc ccgcccttgc aggcctggta cagcgcatcg atcagctcgc
    13621 cgtacacgtc gagttgaagc tgcgcagccg cggcgttgcc gatgcgcacc ggcttggact
    13681 tctcgtagcc cgcgagccag ggcacctcga attccggcag gcggcgctcg ccggcgatgc
    13741 cgtacatgat ctgcatctgc tcggggctgc cggcgaccgc tcgcaccagc cattcgcgcc
    13801 acgcgcgcgc ctcgtcgtag tagcccccgt ccatcagcgc gagcagcgtg agcgtcgcgt
    13861 cgcgcagcca gcagtagcga tagtcccagt tgcggacccc gccgatccgt tccggcaggg
    13921 aggtggtcgg cgccgcgacg atgccgccgg tcggcgcata agtgagcgcc ttcagcgtga
    13981 tgagcgagcg gcgcaccgtc ccgccccatt cgccgcccac ggtgcagcgc gcgctccagt
    14041 gcttccagaa cgcctccgtg tcgtccagcg ccttgccggc atccacctgc cgcggcacgt
    14101 cgaggtgcga agcggtgtgc gtgagcacga aggagagcgt ttcgcccgcg ctcacctcga
    14161 accgcgcgcg cgtcttgcgc tcccggttct cgagcggaac gggcgagcac agcagcgcga
    14221 gatccggacc cgcgaccgcg cgcagggcct tgcgatcggc gctcagcgtg acccagggaa
    14281 cggaagcgcc gtagtcgaag cgcagcacgc ccaccgactg catcgcgacg cgcccgcgcc
    14341 ggccgatgac catacgcacc aggtccgact gcgagctgcg caccggcatg aagtcgacca
    14401 gcgtgactgc cccctcagcg gtctcgaatt cggtttccag gatcagggtg tcggcccggt
    14461 aggaacggct cgctttcgaa acccccactg gagcgatctt ccagtagccg ttctcgggac
    14521 cgccgagaag cgccgcgaag cacgcggccg agtcgaagcg cggccagcac agccagtcga
    14581 tcgagccgtt caggccgacg agtgccgcgg tgtggcagtc gccgatcagc gcgtagtctt
    14641 cgatcctcac ctaggagctg actgttccgg cccgctcttg gttcaggtcc cggctttcag
    14701 cgtgacactc agctcggcgg cctttccctc gcgctgcacc gacagccgga tggtgtcgcc
    14761 gaccttgtgc tcgtccaggc ggttgacgag gcgcgccacg ctgtccaccg ccttgccgtc
    14821 cagcgccgtg atgacatccc cgggcacgag gctcccgtcg gcgccgctgc gcgccgcacg
    14881 cagccccgcc gcctcggcgg gcgagcccgg ctccaccttg agcaccgcaa cgcctttcac
    14941 gccgagctgc ttcgcgatga gctggttcag ttgctcgtcg gtgctgatgc cgagcaccgg
    15001 gcgggtgtac ttgcccttgg cgatgagctg cggcacgacg cggttcaccg tatcgaccgg
    15061 cacggcgaag ccgacgccgg cgtaggcacc cgacgggctg aagatcgcgg tattgatgcc
    15121 gatcaggcgc ccgctcgagt cgagcagcgg gccgcccgaa ttgccgggat tgatcgcggc
    15181 atcggtctga atcaggtgct cgatgaccgt gcgctcgtcc tcggcaggca acgagcggtc
    15241 cagcgcggac accacgcccg tcgtgagcga ccagtccagg ccgaaaggat tgccgatggc
    15301 gaacaccttc tgcccgacct tgaggtcggc cgaggtaccg atcggcaatg ccgtcggctg
    15361 gcgctccggg acgcggatgc gcagcaccgc gatgtcgtgg gccgggctcg cgccgaccag
    15421 ccgcgcggag tagtcgcgcc cgtcgttgag ccgcaccttg gcctcggctg cgccgctgac
    15481 gacgtgattg ttcgtgatga cgtgcccgtc cgcgtcccag acgaagccgg agcccgcgcc
    15541 gcgcgggatg ctgaacatgt tgcgcgacca gcgatcgcgc acgagctgcg tcgtggtgat
    15601 gaacaccacc gagcctttcg agcgctcgaa gagctcgatg gtgcttttct cgtccgaggc
    15661 gaggtcgccg cgggctgcga tggcgcgcgg ctcggcggtc agggcgggag catcggtgcg
    15721 ggcgagccag ggccggccct gccagaccag cacccctcct gccgccgcgg cgacgagcag
    15781 ccagaaactt ctcacctgaa gagcctatcg cagttccgtc ccgcggatct accggaccag
    15841 cccggtcgac gggcactttc tgtaccatcg cgcaacccca tgcgcgacta cgtcgacatc
    15901 gcgttcggcc tcgcggtcct ggtagccgcc ttgttcgcct ccggacacgc ggtgctctac
    15961 aagcgcgagt cgcgctcggc gtcgctgtgg gtgatcctga tctgggtcat gccggtgctc
    16021 gggccgatcc tctacatgct gatgggcgtg aaccgggtgc agcgccgcgc ggcacgcatg
    16081 cgcgcgttca tggtgcggca ccgcgccgac ccccagacga ttcaaggcga gccgcccgga
    16141 acccacctcg cgccgctcgc gcgaatgctg aacaacgtgg tcgagcgacc gctgctgccg
    16201 ggaaactcgg tcgaggtgct ggtcgacggc acccaggcct acccggcgat gctcgaggcg
    16261 atcgagtcgg cgcgcgcgtc ggtgatgctc gcctcctata tcttcgattc cgacggcatc
    16321 ggcgccgagt tcgtcgccgc gctggagcgc gcgcacaagc gcggcgtggc ggtgcgcgtg
    16381 ctggtcgacg acgtcgacgt gcgcttctcg cgccgtccgg tgaccaagcc gctgcgccgc
    16441 gcgggcgtgc cggtcggcgt attcaacccg ccgttcgtgc cggcgcggct gaacgccgtg
    16501 catctgcgca accaccgcaa gatcctggtg gtcgacggca ccgtaggctt caccggcggc
    16561 atgaacatcg acggccggta ctggagtgcg gacgcggcgg gcgagggaaa ggagatctcg
    16621 cgcgacctgc acttccggct gcagggcccg gtgctggcgc agctcgccga agtgttcgcc
    16681 gacgactggc aattcgtcac cggggaagcg ctgcgcggcg cgccctggtt cgtgccgctc
    16741 gagccggcgg gcagcgtgct cgcgcgctgc atcgacgacg gcccagacga gagcaccgag
    16801 ccgctgcgct gggcgatcgt cgccgggctg aaccaggcgc agcgctcggt acgcatcatg
    16861 acgccatact tcgtgcccga cggcgcgctg atcaccgcgc tcgacgtcgc cgcgatgcgc
    16921 ggcgtcgacg tggacatcgt gctcccggcg cggagcgacc tgcctcacgt gcactgggcg
    16981 gcgtggggcc agctgtggca ggtgctggag cgcggctgcc acgtctggtg cacaccgggg
    17041 cctttcgacc actcgaagct gatggtggtg gacggcgcct ggacgctgct cggctcggcc
    17101 aactgggacg cgcgcagcct gcgcctcaac ttcgagctga acgtggagtg ctatagcgtc
    17161 gaattcggcg cgcacatgga ggggctggtg ctcgcgcgca tcaacaccgc caagccggtc
    17221 acgctctcgg acatcaacgc gcgatcgctg cccgtcaagc tgcgcgacgg cgttgcccgc
    17281 cttttcgcgc ccttcctcta attcggggac ggagccctaa ctggttcggg gacggagccc
    17341 taaccagtca accccaagca acgccgggcg ttgtgtcctt atgccaaggg ggccgcggct
    17401 cgttgtgccg ggggttgcgc tacacgtcgt gcagcgtggc cacgatcgcc gagattgctt
    17461 tcagcacgag accgattacc tcgtctatct gtcgagcttg cgagagctat ccacgcagac
    17521 gcaatgtgcg ctgcatgcct actgcctaat gacaaatcac gtgcacctgc tgcttgcgcc
    17581 ttcgagcgtc cagggatgtg ctgtcttgat gcgcaatctg ggccaacgct atgtccagta
    17641 ctttaaccgg cggtatcagc gacgcggcac cctatgggag ggccgctatc actcctgcct
    17701 cgtggattcc gcaccgtacg taatcgcttg ttatcgatac atcgagcgca acccagtccg
    17761 cgcgggcatg gtcgcatcgg cggctgacta cctttggtca agccacaagg gcaatgctgg
    17821 tgaagccaac aacaagctcc tgacgccgca tccagagtat tcggcgcttg ccttgaatga
    17881 cgcgtcgcgg cataccgcat atcaaggcct gtttgccgcg gcggacgatc ccgctttcct
    17941 cgccgcgatt cgcgatgcta cgaatggcgg cttcgcactc gtcggcgagg cgcttaaggc
    18001 cagcttgcct gccaatctcc agcaccggct cgaacgtaag cagcccggcc cccgaccggc
    18061 gcccacagcg caatcagttg atctcgctgc tcaaatgacg ctcgagttag ggctccgtcc
    18121 ccgaaccagt tagggctccg tccccgaatt acttgagcgc tttgtatttg atgcggtggg
    18181 gcttggcggc ttcctcgccc agacgctttc tcttgtcggc ctcgtattcc tggtagttgc
    18241 cgttgaagaa cacccactgc gaatcgtcct cggcggcgag gatgtgggtg gcgatgcggt
    18301 cgaggaacca gcggtcgtgc gagatcacca gcacgcagcc ggcgaattcc agcagcgcct
    18361 cctcgagcgc gcgcagggtc tcgacgtcga gatcgttcga cggctcgtcg agcagcagca
    18421 cgttggcgcc cgacatgagc atgcgcgcga gatgcagccg gccgcgttcg ccgcccgaga
    18481 gctggccgac cggcttctgc tggtcggcgc cgcggaagtt gaagcgggcg aggtaggagc
    18541 gcgcgggcat ctcgaagcgc ccgagcttga gcatgtcctg accgcccgag atatcctcga
    18601 acacggtctt ctcgctctgc agctcctcgc gggactggtc ggcgtgcgcg atcttgaccg
    18661 tcttgccgat gagaatcttt ccgctgtccg gcttttcttt cttgttgatg agcttgaaca
    18721 gcgtggactt gcccgcgcca ttcggcccga tgattccgac gatggcgccg ggcggaacct
    18781 tgaacgagag cttgtcgatc agcagccgct cgccgtagcc cttggagacg ttctggaact
    18841 cgatcacctc gttgccgagg cgctcggcga ccgggatgaa gatctcctgc gtttcgttcc
    18901 tggtttgggt ttcttgagaa gataattctt caaaccgtgc gaggcgcgct ttcgacttgg
    18961 tgcccctgcc cttcgggttc tgccgcaccc attcgagctc ggcctgcatc gccttgatgc
    19021 gcgcgacttc ctggctcgcc tcgacctgca gccgcttctc cttctgctcg agccagctcg
    19081 aatagtttcc ctcccacggg atgccgtacc cgcggtcgag ctcgaggatc cagccggccg
    19141 cgttatccag gaagtatcga tcgtgagtga ccgccaccac cgtgcccgga aagcgctgca
    19201 ggaactgctc gagccactcc acgctctccg cgtcgaggtg gttggtcggt tcatccaata
    19261 acaataggtc cggcttggaa agcagcagcc tgcacagcgc cacgcgccgc ttttcgccgc
    19321 cggataacac gtcgaccttc tgctcccacg gcggcagtcg cagcgcgtcg gccgcgattt
    19381 cgagctcgtc ggcgccgccc gagttgatca tcgcctcgag cttcgcctgc tcctcggcga
    19441 gcttgtcgaa gtcggcgtcg ggctccgagt acgcggcgta gacctcgtcg agtcgcttct
    19501 tcgccgccgc ggtctcgccg agggattcct ccacggcttc gcgcacggtc ttgttttttc
    19561 ctagaacagg ctcttgctcg aggtacccga cgcggatgcc cggcatgcgg accacctcgc
    19621 cttcgtagtt gtcgtcggtg cccgccatga tcttcagcag cgacgacttg cccgagccgt
    19681 tcagcccgag cacgccga
//
LOCUS       DATBSZ010000075        17663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_967197, whole genome shotgun sequence.
ACCESSION   DATBSZ010000075 DATBSZ010000000
VERSION     DATBSZ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17663
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967197"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..656
                     /locus_tag="VIG70_04970"
     CDS             207..656
                     /locus_tag="VIG70_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEY8249938.1"
                     /translation="MRIWVDADACPAVIKDILYRAAERLKIPMTLVANKLLRTPPSPY
                     IRALQVQRGIDVADSHIVRELAAGDLVVTADIPLAADVIAKGGHALNPRGEFYSTENI
                     QERLALRNYLDELRGTGERTGGPAALDNTDRKRFADQLDRFLSKRKP"
     gene            913..1557
                     /locus_tag="VIG70_04975"
     CDS             913..1557
                     /locus_tag="VIG70_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY8249939.1"
                     /translation="MKKGDRYDVSGMVEAQFEPGSRRRVLRNNLGIRSSRAMDVAEAR
                     ALTQAMEAFVAQYDERHRFTADDICGMHRRWLGGIYPWAGAYRQVNISKGGFPFAAAN
                     RVPTLMEGYSREVLARYTPCRPSGVTEVAKALAQTHVELVLIHPFREGNGRIARALST
                     LMGLQAGLPPLDFGSITGKATKDYFMAIQRGMDRDYRLMERLFEAIIERSVAGT"
     gene            1820..2095
                     /locus_tag="VIG70_04980"
     CDS             1820..2095
                     /locus_tag="VIG70_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY8249940.1"
                     /translation="MKYSTRIKPISYVKANAAELIRELAEKREPLVITQNGEATAVMQ
                     DVASYEATQETLALLKILALGRRQVEDGKAIPLAEATRRLRARRAGR"
     gene            2095..2379
                     /locus_tag="VIG70_04985"
     CDS             2095..2379
                     /locus_tag="VIG70_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY8249941.1"
                     /translation="MRFDIRLTPDALADLEEIHDWIALHAAVASLARHPSRGPHPREL
                     LALGIREYRETFFKPYRIVYGVQGRRVDVYLIADGRRDMQALLSRRLLGA"
     gene            complement(2409..3611)
                     /locus_tag="VIG70_04990"
     CDS             complement(2409..3611)
                     /locus_tag="VIG70_04990"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8249942.1"
                     /translation="MTFSTLGLDSTLLDNVTRLGYTEPTPVQREAIPAVLAGGDLLVS
                     SQTGSGKTAAFVLPGLQRLRTRAATRGMGPRMLVLTPTRELALQVQKATHGYCTGQNL
                     LTACLVGGVPFGKQLGAVRKMVDIVIATPGRLKDHLDRGSVDLSRVELLVLDEADRML
                     EMGFQEELDSIIARAPKERQTLLFSATLHGVVGHLANRVTRNAKRVEIARKEETKLDI
                     TQHALIADNDEHKNRMLDCLLRETDVEQTLVFTAMKRTAAELTNSLVIKGFTAGALHG
                     DMHQSDRTRTLNQMRDGRVRVLVATDVAARGIDVPGINLVVNYDAPRQAADYVHRIGR
                     TGRAGRAGIAVTFLGHADRHLVRQIERFTGNKVSLMEIAGLEPRTRGERKPDSGRREG
                     YSARRFSR"
     gene            complement(3766..4005)
                     /locus_tag="VIG70_04995"
     CDS             complement(3766..4005)
                     /locus_tag="VIG70_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249943.1"
                     /translation="MKATLSLKARRHAITRIIEARFSVSAFEDVRRKNPQYWRSPRND
                     IARGIYGEAMREKQRLIGSSDEMLEAESLALRLAS"
     gene            complement(4189..4908)
                     /locus_tag="VIG70_05000"
     CDS             complement(4189..4908)
                     /locus_tag="VIG70_05000"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEY8249944.1"
                     /translation="MAPRNLLHAIALGALATVAHPFTFAARAASAAGSSFGRTARRFI
                     DSRGAAARSKLDTAQWTPELLKRLEWRRFEEVCAAYFEALGFRADLAGAGADGGVAIN
                     VYEQGAKNASIIVQCRPWTAHHIGIKPVRELRGAMASGHVSEGVLMTSGKFTKEARDF
                     AGKEGVSLIDGTDLAAKLAGLVPEKALAVYKLATQGDFLTPTCPSCAIKMISRKSTAH
                     GRPYWGCRNYPACKHTFFEAL"
     gene            4959..5423
                     /locus_tag="VIG70_05005"
     CDS             4959..5423
                     /locus_tag="VIG70_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249945.1"
                     /translation="MADYLRDELGKLLNQHDEQRRAVLAREQKVKDDDALFVAGFADL
                     RRSVIRPAFETAAAMLAERGHKVTIAEQEFSVGAGAKVTEAEISLRIVVSGAKEENAR
                     AFAISTRHYNKTVLINAGKPLEGKTGTYALENIDRHRVEEELVRFVAGIVAG"
     gene            complement(5451..7139)
                     /locus_tag="VIG70_05010"
     CDS             complement(5451..7139)
                     /locus_tag="VIG70_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8249946.1"
                     /translation="MPLLTLADAELHYGDLPLLDRAALAVEAGERIGLIGRNGTGKSS
                     LLGILAGTIALDDGELKKMDGLKIVRVEQEPGVPESSGIEAHKLEKFLHLFKVDQTVS
                     GGELKRAALAHAFAQEPDLLLLDEPTNHLDIDGILVLEQLLLRTPAAIVVTHDRAFLD
                     RVATRIVELDRGRLLSFAGNFSSYEKTKAAQLAAEAVTQRKFDKFWAQEEAWIRKGIE
                     ARRTRNEGRVRRLERLRDERAARRSRIGDVKIAIGEGQRSGKLVSEFLKVGKSFHDRV
                     IVKDLDLIVSRGDRLGLVGPNGAGKTTLLNLILGTLAPDSGRVRLGTNVQVAYFDQMR
                     EVLDPEKTVAESIDSNLPAGKHVMSYLGDFLFPPRRANSKVSMLSGGERNRLLLARLF
                     AKPANVLVLDEPTNDLDIESLELLEAALQDYAGTILLVSHDRAFLDNVVTQTLASEGD
                     GKWKEYAGGYSDYLRQRPAVVAPLTRGDASAPRERGVGSRKLSYKETRELAALPAEIA
                     ALEAEQKQLAEKMAAPDYYRQPPDLLRADQGRSAEIEALLTEKLERWEALEAKV"
     gene            7224..7469
                     /locus_tag="VIG70_05015"
     CDS             7224..7469
                     /locus_tag="VIG70_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY8249947.1"
                     /translation="MKNVTITLDQDTTARARVKAAERNMSLSRYIGEVLRKELRHGDE
                     YEAAYRAWRKSKPFPLKGPPEPYPKRDEIYDRIIRRR"
     gene            7444..7956
                     /locus_tag="VIG70_05020"
     CDS             7444..7956
                     /locus_tag="VIG70_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8249948.1"
                     /translation="MTGSFVDANVFIYALDPRDARKHQRARDWVDALWTDRLGRTSAQ
                     AIAEAYSVSTRKLGVAPHTAWGELERFFAWNPQPVDEPLLRRAREIELRYKLSWWDSM
                     IVAAAQLQDCVLLLTEDLQDGAAFGAVTARSPFTLEVREAAAAYAVERRGPERYRPRG
                     RPRRAAAPAP"
     gene            complement(8032..8910)
                     /locus_tag="VIG70_05025"
     CDS             complement(8032..8910)
                     /locus_tag="VIG70_05025"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8249949.1"
                     /translation="MTAQYDPKARYEVEVQEMEYRRSPKGRPLMARLYQPKRKNEGSG
                     PFPVLLDLHGGAWNNKDRFANETMARAVAASGVLVASIDMTLASDAPYPASVQDANYG
                     VRWLKWKAPGWHGDPSTLGILGSSTGGHMGELVGMKPRDPKYSAFPLAGAPALDASVA
                     YVAARSPISDPFARFQQAEKMKRAEMMQNTKNYWKPWETIYEGNPQQILERKEKVSLP
                     PLLIMQGSLDDNVLPALQEKFAATYRAAGGECQLEIFEGCEHLWVDKPGPMTDKAHEM
                     VKAFIARNLGKLRQAA"
     gene            9116..9301
                     /locus_tag="VIG70_05030"
     CDS             9116..9301
                     /locus_tag="VIG70_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249950.1"
                     /translation="MQKLPELHLVRASEAAAASNVVHISRLRSHGPASLYAVGRSWPL
                     QHAWRDLVLSLMTKPTP"
     gene            9298..9618
                     /locus_tag="VIG70_05035"
     CDS             9298..9618
                     /locus_tag="VIG70_05035"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8249951.1"
                     /translation="MNAWIASAMLAALCAFTNASAQEKLQEKGGGFNDAVITELVTSA
                     LDQDPVLRQMNIAVQTQGGVVRLSGFVDSMAQVDRAGALARRIEGVSSVRNTIRVTNR
                     PSRA"
     gene            complement(9724..10455)
                     /locus_tag="VIG70_05040"
     CDS             complement(9724..10455)
                     /locus_tag="VIG70_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8249952.1"
                     /translation="MLLLDLSPLALAYCILVLLLNFTLRGSLGFGGALGLPLLALAIP
                     VKVIAPAWSLIGIVSSVAIVGRSRRHIDWREFVGLLPGCAIGILAGLFAFKALDAKLL
                     ARALGVFIIAYAAYSWWKPRFELRLLRPAASVLSGIVGTLFGAMATIFFAMYLDAGKM
                     TKDAFRATMSAMILTISVARTLGYAAVGELTLDSLILCAASIPAMGVGLLVGDRLHTG
                     LSPIAFQRFVCIALAACGVALWGQG"
     gene            10599..11159
                     /locus_tag="VIG70_05045"
     CDS             10599..11159
                     /locus_tag="VIG70_05045"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEY8249953.1"
                     /translation="MKLEMHQNSLFAVLLRSPWWVSLLVAIGLAAVLRMFLPLEFALF
                     GGLPFAAIAAVAGWRQLKRPGPKRIAATLARARALSADAFCAALEEGFRRQGHGTRRG
                     EGAADLVLTHKGIVSVVGCRRWKAARTGIEPLREFDAATSESGAHKRIYVAVGEVSDS
                     ARELAAKKGIRLVQAEELAGLLSALR"
     gene            11177..12442
                     /locus_tag="VIG70_05050"
     CDS             11177..12442
                     /locus_tag="VIG70_05050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8249954.1"
                     /translation="MSPRSAALGVIALTLAISYGIWYSYSVILVALLAEFGWSRSVLA
                     GAFSVFTLVHGGINPVVGALCARFRPLRVMAAGGAAMGLALFADSFVTTPLGFYAAFG
                     VLTAIAVAAGGWVPALVFVQGQFKERLGLSIGIISSGVGVGMVLVVPFTQLLIDAYGW
                     RVAFRVLGVIAFAWIVPSSLWLRWWMLRHREIRGQSPNSFSLTQKQNSGSDPMTLREA
                     MRTQPFWLLMAAFFFGNISSQTLHVHQVAYLVDHGLSAIVAATVVGVVGFASIFGKIG
                     GGWLSDRMEREVVYAGGIAIMVASAFALLAIGTASSVWAAYGYAVLLGVGYSATASLI
                     PAMLSDRFSGRHFGAIVGVCLIGSSTGSAVGPWLAGMLYDATSSYTAAFLIAAACGVI
                     AGAAGWRARALRIRAPISSRSPEPTTRAP"
     gene            complement(12399..13187)
                     /locus_tag="VIG70_05055"
     CDS             complement(12399..13187)
                     /locus_tag="VIG70_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY8249955.1"
                     /translation="MPIVPNHALRQLRAGKLAIGLGVNQARTVGIATMAKTAGFDWLF
                     IDAEHGSLDPDTAAQISTAALAAGVSPIVRVAGFEHWHASRLLDNGAQGIVFPHVDDA
                     ASAKRVADHCRYPPVGKRSMGGALAQLGFASVPVGEAARMVNEETLVVVMIESPQGVQ
                     NADEIAAVKGVDALLIGTNDLCFEMGIPGQFNDPKVKDAYTKVIAACRKHGKFPGMGG
                     MYTAELLERHIGMGVQLILSGSDFSLLMQGATARASLVRGFEKK"
     gene            13292..14689
                     /locus_tag="VIG70_05060"
     CDS             13292..14689
                     /locus_tag="VIG70_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY8249956.1"
                     /translation="MKAKVAYVALLALAIVGTGVGVSQLPLQFPHANAQAQPAAVQTL
                     PDFAAIVEANKAAVVNITASSAAKPQAEVPELPEEFRNSPFGEFFRRFQGPQPNVPRQ
                     GMGSGFIVESDGTVLTNAHVVEGADEVRVRLTDRREFKGKVVGVDKPSDIAVVKIEAR
                     GLPVVRLGDPSKVRVGEWVLAIGSPFGFENTVTAGIVSATSRSLPEGTYVPFIQTDAA
                     VNPGNSGGPLFNLKGEVIGIASQIYSRTGGYQGLAFAIPIDVAANVKEQLVKHGRVER
                     GRIGVAIQEVTQSLAQSFGLDRPRGALVAQVDNDGPAAKGGIKAGDVLLGVNGKAIDR
                     SAELPPLVAAVKPGTKASFDVWRDGRKQVLEVTVGALEPEKVAAVGPQGGGAPTGKLG
                     LAVRPSEDGLVVENATGAAARAGIRPGDVVTAVNGRPVKSVDDLRAAADNAKGTVALL
                     VRRGEASIFVPVDVG"
     gene            complement(15025..15396)
                     /locus_tag="VIG70_05065"
     CDS             complement(15025..15396)
                     /locus_tag="VIG70_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249957.1"
                     /translation="MSQRYPGDPRKRLNRAFALLATHVTDPEALGCGMANAAVGIIGQ
                     GRPARQAIEAHKAKLHAKLAELCVRSGASEPELLAEQLFLLIQGAQAVLILGVHSPAR
                     NAGPAAASLIDSRLAAAALPS"
     gene            complement(15483..15833)
                     /locus_tag="VIG70_05070"
     CDS             complement(15483..15833)
                     /locus_tag="VIG70_05070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004537279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY8249958.1"
                     /translation="MQQPYDFIPGGLIKEDLVVGSGARAEAGNDVVVHYVGWMQGGKQ
                     FDSSRARRDPLDLALGAGDVIRGWDEGIPGMRVGGKRRLTIPPALAYGSHGLAGVIPP
                     EATLVFEVELLEVS"
     gene            15922..>17663
                     /locus_tag="VIG70_05075"
     CDS             15922..>17663
                     /locus_tag="VIG70_05075"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY8249959.1"
                     /translation="MAATAFERLNPRQREAACAGPAPLLIVAGAGTGKTNTLAHRVAH
                     LVLQGASPEKILLLTFTRRAALEMTRRASRIVAEVRKDVRLPWSGTFHSVANRLLRLY
                     SKRISLAESFSVLDRGDASDLMDVVRHERGLSKSEKRFPRKDTCLAIYSHRVNTQRPL
                     ADTLALMFPWCAEWEAELTGLFRNYVERKIQNQSLDYDDLLLYWHAMMQDEALAAEIG
                     VRFDHVLVDEYQDTNVLQAEILQRLKPSGEGVTVVGDEAQAIYSFRAATVDNIRNFQK
                     QGRTTVSLEENYRSTQQILDAANALIGKSLYSRTKLGAKPRYVTVADDEGQAQYVVTR
                     VLEARERGVLLRNQAVLFRASHHSDLLELELVRRNIPYVKYGGLKFLEAAHVKDLLAV
                     LRWADNPRNRVAAFRALQLLPGVGPAGAARVFTRFEAEGFSWKPLKTGSAGLDELMLY
                     LGEAPWEGQVQRVREWYLPHLERIYDAAQVRAGDLLQLEKISGQFADRSTFLTEMALD
                     PPSATSDLSGEPYLDEDYLVLSTVHSAKGQEWEAVHILNVADGNFPSEFSTGRPELID
                     EERRLLYVAMTRAK"
BASE COUNT         2814 a         5668 c         6293 g         2888 t
ORIGIN      
        1 gctcgatcgt cccgataccg ccggctgcaa ctggtcgtac acgctcgtgc tcgacacggc
       61 gggcgtgccg gcggaggtct atgggctcgc ctacgctcag gtgatcggga tggcgcggag
      121 tagctggaac ctgcggtaaa aattcggggt cagggcccta aacctgctgg cgcgtagctg
      181 gaccagattc gagccctgac cccaacatgc gcatctgggt tgacgctgac gcgtgtcccg
      241 cggtgatcaa ggacatcctc taccgcgcgg cggagcgctt gaagattccg atgacgctgg
      301 tggcgaacaa gctgctgcgc acgccgccgt cgccgtacat acgcgcgctg caggtgcagc
      361 gcggcatcga tgtcgcggac agccacatcg tgcgcgagct cgcggcgggg gatctggtcg
      421 ttactgcgga tataccgctg gcggccgacg tgatcgccaa gggcgggcac gcgctcaatc
      481 cgcgtggcga gttctattcg acggagaaca tccaggagcg cctggcgctg cgcaactacc
      541 tggacgagct gcgcgggacg ggggagcgga ccggcggtcc ggcggcgctc gacaataccg
      601 atcgcaagcg cttcgcggat cagctcgatc ggtttctttc gaagcgaaaa ccctaaaagc
      661 tatctgctgt accgtggccg ctgctcgggt ctgccgtccg cctcggccac ccgcgctggt
      721 gaatacgatg tgctggtttg tggcggctac gcacaccgct tcttcggcca ggacttccgc
      781 acgccggagg gcgtagggtg gtgaagtgcc cgtaggtcaa cgcgtgccgc cacgccggcg
      841 ttgagatcgg gcatgctccc gaaatcccaa gtcgctgcgc gcgccgggcg cgactacccc
      901 ggtaattgac gagtgaagaa gggcgaccgc tacgacgttt ccggaatggt cgaggcgcag
      961 ttcgagccgg gctctcgccg gcgcgtcctt aggaacaatc tcggcatcag gtcttcgcga
     1021 gcgatggacg ttgccgaagc aagggcgctg acgcaggcga tggaggcgtt cgtcgcgcag
     1081 tacgacgagc gtcatcgctt caccgcagac gacatctgcg ggatgcaccg ccggtggctg
     1141 ggcggtatct atccgtgggc gggcgcatac cgccaggtga atatcagcaa gggcggtttt
     1201 cccttcgcgg cggcgaaccg cgtcccgacg ctgatggagg gctacagtcg tgaagtgctg
     1261 gcgaggtaca cgccatgtcg gccgagcggc gtgacggagg tggccaaggc gctggcgcaa
     1321 acgcacgtcg agctggtgct gatccatccg ttccgggaag gaaacgggcg cattgcgcgt
     1381 gcgctctcca ccctcatggg attgcaggca gggctgccgc ctctcgactt cgggtcgatt
     1441 accgggaaag cgacgaagga ctatttcatg gcgatccagc gtggcatgga ccgcgactac
     1501 cggctgatgg agcggctttt cgaggcgatt atcgagcgga gcgttgccgg gacctgagcg
     1561 atgcgacgcg tccgtgccgc ttcagcgttc gcgcgatggt ctcgggcttc ctgccggttt
     1621 cgaccgaggt actggtcgcg acgttcgtga taaggccggc acgccgcctc ctcgcctcgc
     1681 ggaggtgagc attggtctcg atgagcgtct tcttcggcac ggcggcatct taacatcccg
     1741 ggcttgcggg gcgaaccgag gatgggcagc agacgccgcg ggattgcggc cggaccgaaa
     1801 caggactaaa ttcggtctca tgaaatactc tacccggatc aagcccatca gctacgtgaa
     1861 ggcgaacgcc gccgagctga tccgcgagct ggccgagaag cgcgagccgc tcgtcatcac
     1921 ccagaacggg gaagcgaccg cagtcatgca ggacgtcgcg agctacgagg cgacgcagga
     1981 gacccttgcg ctgctgaaga tcctggcgct cggcaggcgc caggtcgagg acggcaaggc
     2041 cattccgctc gccgaggcta cgcgccggct gcgcgcgcgc cgagccgggc gctgatgcgc
     2101 ttcgacatcc ggctgacgcc ggatgcgctc gccgatctcg aggagattca cgactggatc
     2161 gcgctgcacg ccgctgtcgc gtcgcttgcc cggcatccga gccgtgggcc gcatccaaga
     2221 gagctgctcg cgctcggcat acgcgagtac cgcgaaacat ttttcaagcc ttatcggatc
     2281 gtttacggcg tgcaaggacg ccgggtcgac gtgtacctga tcgccgatgg ccggcgtgac
     2341 atgcaggcct tgctgtcgag aagattgttg ggcgcgtgaa aaaaatcggg gtcagacccc
     2401 ggtgtctcct acctgctgaa ccgcctcgcc gagtagcctt cgcgccgccc ggagtcaggc
     2461 ttgcgctcgc cccgcgtccg cggctcgagg ccggcgatct ccatcaggct gaccttgttg
     2521 ccggtgaagc gctcgatctg gcgcaccagg tggcggtcgg cgtggccgag gaaggtcacg
     2581 gcgatgccgg cgcgcccggc gcggccggtg cggccgatgc ggtggacgta gtctgccgcc
     2641 tggcgcgggg cgtcgtagtt caccaccagg ttgatgcccg gcacgtcgat gccgcgcgcg
     2701 gcgacgtcgg tggccaccag gacgcgcacc ctgccgtcgc gcatctggtt cagcgtgcgg
     2761 gtgcggtcgc tctggtgcat gtcgccgtgc agcgcgccgg cggtgaagcc tttgatcacc
     2821 agcgagttcg tcagctcggc cgcggtgcgc ttcatggcgg tgaagacgag ggtctgctcg
     2881 acgtcggtct cgcgcagcag gcaatcgagc atgcggttct tgtgctcgtc gttgtcggcg
     2941 atgagggcgt gctgggtgat gtcgagcttg gtttcttcct tgcgcgcgat ctcgacgcgc
     3001 ttcgcgttgc gcgtgacgcg gttcgcgagg tggccgacca caccgtggag cgtcgccgag
     3061 aagaggagcg tctggcgctc cttcggcgcg cgcgcgatga tcgaatcgag ctcttcctgg
     3121 aagcccatct cgagcatgcg gtcggcctcg tcgagcacca atagctcgac gcggctgagg
     3181 tcgacgctgc cgcggtccag gtggtccttg aggcggccgg gcgtggcgat gacgatgtcg
     3241 accatcttcc ggacggcccc gagctgcttg ccgaacggca cgccgccgac caggcaggcg
     3301 gtgaggaggt tctggccggt gcaatagccg tgcgtggctt tctgcacctg cagcgccagc
     3361 tcgcgcgtcg gcgtgaggac gagcatgcgc gggcccatgc cccgggtcgc cgcccgcgtg
     3421 cgcaggcgct gcagccccgg caggacgaag gcggcggtct tgccgctgcc ggtctgcgac
     3481 gagaccagca ggtcgccgcc cgccagcacc gcgggaatgg cttcgcgctg caccggcgtc
     3541 ggctcggtgt agccgaggcg ggtgacgtta tccaacaggg tcgaatcgag gccgagcgtc
     3601 gagaaagtca tcggatctcc ggtggcgccg accaaaaccg ctttgccgga ccgaaaggaa
     3661 atctttcgac gggactgcga gagggaggcg ggactaaaac agcgagggcg gactgtacgc
     3721 taaagcgccc cgtctggcga ctactttatc cagtggcggg agccgctagc tcgcgaggcg
     3781 gagcgccagg ctctcggcct cgagcatctc gtcgctcgag ccgatcagcc gctgcttctc
     3841 gcgcatcgcc tcgccgtaga tgccgcgcgc gatgtcgttg cgcggcgagc gccagtactg
     3901 cggattcttg cgccgcacgt cctcgaaggc gctgacgctg aagcgcgcct cgatgatgcg
     3961 ggtgatggcg tgccggcgcg ccttgagcga gagcgtcgct ttcatgcccc agtatgcgcc
     4021 ccgcgcacgc gcggctctgt gcgttggcga acccgcgaat ccatcatttg gtggacagac
     4081 agcgcccggc gcttcttcca caatggggtc cccggagcca ttcacccttc ccccattggg
     4141 tggggctcta tgtgagaggc tgccttcggg cagcctcttt tttttcggct acagcgcttc
     4201 gaagaaggtg tgcttgcagg cggggtagtt gcggcagccc cagtacggcc ggccgtgcgc
     4261 ggtgctcttg cgcgagatca tcttgatggc gcacgacggg caggtgggcg tcaggaagtc
     4321 gccctgggtc gcgagcttgt agaccgcgag cgccttttcc ggcaccagcc cggcgagctt
     4381 ggcggccagg tcggtgccgt cgatcaggct gactccttcc ttgccggcga agtcgcgcgc
     4441 ttccttggtg aacttgcccg acgtcatcag cacgccttcg ctcacgtgcc ccgacgccat
     4501 cgcgccgcgc agctcgcgca ccggcttgat gccgatgtgg tgcgcggtcc acggccggca
     4561 ttgcacgatg atggaggcgt tcttggcgcc ctgctcgtac acgttgatgg cgacgccgcc
     4621 gtcggcgccg gcgccggcaa ggtcggccct gaacccgagc gcctcgaaat aggcggcgca
     4681 cacttcctcg aagcgccgcc actcgagccg cttcagcagc tccggggtcc attgcgcggt
     4741 gtcgagcttc gagcgcgcgg cggcgccgcg cgagtcgatg aatcgccggg cggtgcggcc
     4801 gaacgaggac ccggcggcgg acgcggctct ggcggcgaag gtaaaagggt gcgcgaccgt
     4861 ggcgagggcg ccgagggcaa tcgcgtggag caggtttctt ggcgccatgg ccgagtttaa
     4921 ccgcggattg cccttcgcag ggcgtctaga atgcggcggt ggcggactac ctgcgcgacg
     4981 agctcggcaa gctgctgaac cagcacgacg agcagcgccg cgcggtgctg gcgcgcgagc
     5041 agaaggtcaa ggacgacgat gcgctcttcg tcgcgggctt cgcggacctg cggcgcagcg
     5101 tgatccggcc ggcgttcgag accgccgcgg cgatgctggc ggagcgcggc cacaaggtca
     5161 ccatcgccga gcaggagttc tcggtcggcg ccggagccaa ggtcacggag gccgagatct
     5221 cgctgcgcat cgtggtttcg ggagcgaaag aagaaaacgc gcgcgcgttc gcgatcagca
     5281 cccggcacta caacaagacg gtgctgatca acgccggcaa gccgctcgag ggcaaaacgg
     5341 gcacctacgc gctggagaat atcgaccgcc accgcgtcga ggaggagctc gtcaggttcg
     5401 tcgccggcat cgtcgccggc taggcccccg ccttcgcggg ggtgacttgt tcagaccttg
     5461 gcttcgagtg cttcccagcg ctcgagcttt tccgtcagca gggcctcgat ctcggcgctg
     5521 cggccctgat cggcgcgcag caggtcgggc ggctggcggt agtagtcggg cgcggccatc
     5581 ttctccgcga gctgtttctg ctcggcttcc agcgcggcga tttcggcggg gagggcggcg
     5641 agctcgcggg tttctttgta agacaacttc cgagaaccaa ccccccgctc gcgcggagcg
     5701 ctcgcatccc cccttgtcag gggggcgaca acagcagggc gctggcgcag atagtcgctg
     5761 tagccgccgg cgtattcctt ccacttgccg tcgccctcgg aagcgagcgt ttgggtgacc
     5821 acgttgtcga ggaaggcgcg gtcgtgggag acgagcagga tggtgccggc gtagtcctgc
     5881 agcgcggcct cgaggagctc gagggactcg atgtcgaggt cgttggtcgg ctcatccagc
     5941 accagcacgt tggcgggctt ggcgaagagg cgcgcgagca gcaggcggtt gcgctcgccg
     6001 ccggagagca tcgagacctt cgagttggcg cggcgcggcg ggaagagaaa atcgccgagg
     6061 tagctcatca cgtgctttcc ggcgggaagg ttggaatcaa tactctctgc aaccgttttc
     6121 tcggggtcca gcacttcgcg catctggtcg aagtacgcca cctgcacatt ggtccccagg
     6181 cgcacgcggc cggagtcggg cgcgagcgtg ccgaggatca ggttgaggag cgtggtcttg
     6241 ccggcgccgt tggggccgac caggccgagg cggtcgccgc gcgagacgat gagatcgagg
     6301 tcttttacga tcacccgatc gtggaacgac ttacctactt taagaaactc tgaaacaagt
     6361 ttgcccgagc gctggccctc gccgatggcg attttgacgt cgccgatgcg cgagcggcgt
     6421 gcggcgcgct catcgcgcag gcgctccagg cgccggacgc ggccttcgtt gcgcgtgcgg
     6481 cgcgcctcga tgcccttgcg gatccaggcc tcctcctgcg cccagaactt gtcgaacttg
     6541 cgctgcgtga cggcctcggc ggcgagctgc gccgccttgg tcttctcgta ggaggagaaa
     6601 tttcccgcga aggacaggag ccgcccgcgg tcgagctcga cgatgcgcgt cgcgacgcgg
     6661 tcgaggaagg cgcggtcgtg ggtcaccacg attgccgctg gggttcttaa gagcaattgc
     6721 tctaaaacca aaatgccatc gatgtcgagg tggttggtcg gctcgtcgag cagcagcagg
     6781 tcgggctcct gcgcgaaggc gtgggcgagg gcggcgcgct tgagctctcc gcccgacacg
     6841 gtctggtcga ctttgaaaag atgcagaaac ttctcgagct tgtgcgcctc gatgccggag
     6901 gactccggca cgccgggctc ctgctcgacg cgcacgatct tcaagccatc catcttcttc
     6961 agctcgccgt cgtcgagcgc gatcgtgccg gcgaggatgc cgaggagcga ggacttgccg
     7021 gtgccgttgc ggccgatgag gccgatgcgc tcgccggcct ccaccgcgag cgcggcgcgg
     7081 tcgaggagcg ggaggtcgcc gtagtgcagc tcggcgtcgg cgagggtgag gaggggcacg
     7141 cgccgaatat ataaggtcag cgaaacggtc agcgaaacag ggcggctcgg taattacacg
     7201 gccttcgtgt aatatcggca gccatgaaga acgtgacgat cacgctcgac caggacacga
     7261 cggcgcgggc gcgcgtcaag gcggccgaac gcaacatgag cctgtcgcgc tacatcggcg
     7321 aggtgctgcg caaggagctg cgccacggcg acgagtacga ggcggcgtac cgcgcctggc
     7381 gcaagagcaa gccttttccc ctgaaggggc cgccggagcc ctaccccaag cgcgacgaga
     7441 tctatgaccg gatcattcgt cgacgctaac gtcttcatct acgccctgga tccgcgggat
     7501 gcgcgcaagc accagcgcgc gcgggattgg gtcgatgcgc tatggaccga tcgtcttggc
     7561 cgcaccagcg cccaggcgat cgccgaggcg tactcggtct ccactcgcaa gctcggcgtc
     7621 gcgccgcaca cggcgtgggg agagctcgag cggtttttcg cctggaatcc ccaacccgtc
     7681 gacgagccgc tgctgcgccg cgcgcgcgaa atcgagcttc gctacaagct ctcgtggtgg
     7741 gacagcatga tcgtcgccgc cgcacagctg caggactgcg tgctgctgct cacggaagac
     7801 ctgcaggacg gggctgcgtt cggcgccgtg accgcgcgca gcccgttcac gctcgaggtg
     7861 cgcgaagcgg cggctgccta cgcggtcgaa cgccgcggcc ccgagcggta ccggccccgg
     7921 ggcaggccgc ggcgcgcagc ggcgcccgcg ccatgaggcg atggaaattc ggggtcaggg
     7981 ccgtaaacct gctgacgcga agctggaaca gattccagcc ctgaccccag ttcaggcggc
     8041 ctggcgaagc ttcccgagat tgcgcgcgat gaaggccttc accatctcgt gcgccttgtc
     8101 ggtcatcggc ccgggcttgt ccacccacag gtgctcgcaa ccctcgaaga tctcgagctg
     8161 gcattcgccg ccggcggcgc ggtaggtggc cgcgaacttc tcctgcagcg ccggcagcac
     8221 gttgtcgtcg agcgagccct gcatgatgag gagcggcggc agcgagacct tctccttgcg
     8281 ctcgaggatc tgctgcgggt tgccctcgta gatggtctcc cacggcttcc agtagttctt
     8341 cgtgttctgc atcatctcgg cgcgcttcat tttctcggcc tgctggaagc gcgcgaaggg
     8401 gtcgctgatc ggcgagcgcg cggcgacgta ggcgacgctg gcgtcgagcg ccggcgcccc
     8461 cgcgagcggg aaggcgctgt acttcgggtc gcgcggcttc atgccgacga gctcgcccat
     8521 gtgtccgccg gtggagctgc ccagtatccc gagggtcgag gggtcgccgt gccagccggg
     8581 cgccttccac ttcagccagc gcacgccgta gttcgcgtcc tgcaccgaag cggggtaggg
     8641 cgcatcggag gcgagggtca tgtcgatcga ggcgaccagc acgccgctcg cggctaccgc
     8701 gcgcgccatg gtctcgttgg cgaagcggtc cttgttgttc caggcgccgc cgtgcaggtc
     8761 gaggagcacc ggaaagggcc ccgagccctc atttttcctt tttggctggt agaggcgcgc
     8821 catcagcggg cggcccttcg ggctgcgccg gtattccatt tcctgcactt cgacttcgta
     8881 gcgggctttg gggtcgtatt gagcggtcat ggtgaagcga ttgtaaggcg gcgcggccgg
     8941 cgcgcgcgcc cgccgggtat tccttgaccc cgctgaagcc ggcgcgtatg gtggggcgca
     9001 cgttccggaa gtaacctgtc gtccaccgcc gctcgcggcg attctcagca gacctcccgc
     9061 tactcagcag tacgcccggc cgagccgccg ctttcaggcg gacagaaagg acgcgatgca
     9121 aaagctgccc gagctgcacc tggtgcgcgc gagcgaggcc gccgcggcga gcaacgtggt
     9181 tcatatcagc cgcctccgct cgcacgggcc cgcgagcctt tacgccgtcg ggcgatcgtg
     9241 gccgctgcag cacgcgtggc gcgacctcgt gctgagcctg atgacgaagc ccacgccgtg
     9301 aacgcctgga tcgcgagcgc gatgctcgcc gcgctctgcg ccttcaccaa cgcgagcgcg
     9361 caggagaagc tgcaggaaaa gggcgggggc ttcaacgacg cggtgatcac cgagctcgtg
     9421 acgagcgcgc tcgaccagga tcccgtgctg cgccagatga acatcgccgt acaaacacaa
     9481 ggcggcgtgg tccgcctcag cggcttcgtc gactcgatgg cccaggtgga ccgggccggg
     9541 gcgctcgcgc gccggatcga gggcgtgagc agcgtacgca atacgatccg cgtgacgaac
     9601 cggccatcgc gcgcatgagc gccgggcgcg cgcccggcac ctgaaattcg gggtcagggc
     9661 cggaattcgt ttgaactacg ttcaaccaga attccagccc tgaccccctc agccctgacc
     9721 ccctcagccc tgaccccata gcgccacgcc gcacgcggcg agcgcgatgc aaacgaagcg
     9781 ctggaaggcg atcggggaga ggccggtgtg gaggcggtcg ccgaccagga ggccgacgcc
     9841 catcgccggg atcgaggcgg cgcagaggat cagcgagtcg agcgtgagtt cgcccaccgc
     9901 ggcatagccg agggtgcgcg cgacgctgat ggtgaggatc atcgccgaca tggtggcgcg
     9961 gaacgcgtcc ttggtcatct tgccggcgtc caggtacatc gcgaagaaga tggtcgccat
    10021 ggcgccgaac agggtgccga cgatgccgga cagcacgctc gccgcgggcc ggagaagcct
    10081 cagctcgaaa cgcggcttcc accacgaata tgcggcgtac gcgatgatga agacgccgag
    10141 cgcgcgcgcg aggagcttcg catcgagcgc cttgaacgcg aacaggcctg cgaggatgcc
    10201 aatggcgcag ccgggcagca ggccgacgaa ttcgcgccag tcgatgtgcc ggcggctgcg
    10261 gccgacgatc gcgacggagg agacgatgcc gatgaggctc cacgcgggcg cgatgacctt
    10321 caccggaatg gcgagcgcga ggagcgggag tcccagcgcg ccgccgaagc cgagcgagcc
    10381 gcgcagggtg aagttcagca gcagcaccag gatgcagtag gcgagcgcga ggggggagag
    10441 gtcgaggagg agcaagatgc aattctagga aattcgggga cggagcccta tctatctcgc
    10501 ggcgtcaacg cgcgtgacgc catgcatatc gccgctgtaa ccgcgacctg ccctcggcgc
    10561 gggataattc gggctctgac cccgaattcc aatcacgcgt gaagctggag atgcaccaga
    10621 actcgctgtt cgcggtgctg ctgcgctcgc cgtggtgggt gagcctgctg gtggcgatcg
    10681 gcctggcggc ggtactgcgg atgttcctgc cgctcgagtt cgcgctgttc ggcgggctgc
    10741 cgttcgcggc gatcgccgcg gtggccggtt ggcggcagct caaaaggccg gggccgaagc
    10801 gcatcgccgc cacgctggca cgcgcccgcg cgctttcggc ggacgcgttc tgcgccgcgc
    10861 tcgaggaagg gttcaggcgc cagggccatg ggacccggcg cggcgagggc gcggcggacc
    10921 tggtgctcac gcacaaggga atcgtgagcg tggtgggctg ccggcgctgg aaggcggcgc
    10981 gcaccggcat cgagccgctg cgcgagttcg acgccgcgac gagcgagagc ggcgcacaca
    11041 agcggatcta cgtcgcggtt ggcgaggtca gcgacagcgc ccgcgagctc gcggcgaaga
    11101 agggaatccg cctggtgcag gcggaggagc tcgccgggct gctctccgcc ctgcgctaga
    11161 ttcggggctc ggacccatga gcccccgctc cgcagccctc ggcgtcatcg cgctaacgct
    11221 cgcgatctcc tatggcatct ggtactccta cagcgtcatc ctggtggcgc tgctcgccga
    11281 gttcggctgg agccgctcgg tgctcgcggg cgcgttctcg gtgttcacgc tggtgcacgg
    11341 cggcatcaac ccggtggtcg gagcgctgtg cgcgcgtttc cggccgctgc gcgtgatggc
    11401 ggcgggcggg gcggcgatgg ggctcgcgct cttcgccgac agcttcgtca ccacgccgct
    11461 cgggttctac gccgccttcg gcgtgctcac cgcgatcgcc gtggcggcgg gcggctgggt
    11521 gccggcgctg gtcttcgtgc agggccagtt caaggagcgg ctggggctct cgatcggcat
    11581 catcagctcg ggcgtcggcg tggggatggt gctggtggtg cctttcacgc agctgctgat
    11641 cgacgcgtac ggctggcggg tcgcgtttcg cgtgctgggg gtgatcgcgt tcgcgtggat
    11701 cgtgccgtcg tcgctgtggc tgcggtggtg gatgctcagg cacagggaaa ttcggggtca
    11761 gagcccgaat tcgttttcgc tgacgcaaaa acagaattcg ggctctgacc ccatgacgct
    11821 ccgggaggcg atgcgcacgc agcctttctg gctgctgatg gcggcgtttt tcttcggcaa
    11881 catcagctcg cagaccctgc acgtgcacca ggtggcgtac ctcgtggacc acgggctgag
    11941 cgcgatcgtg gcggcgacgg tggtgggcgt ggtgggcttc gcgagcatct tcggcaagat
    12001 cggcggcggg tggctctcgg accgcatgga gcgggaggtg gtgtacgcgg gcgggatcgc
    12061 catcatggtg gcgagcgcct ttgcgctgct cgcgatcggg acggcctcct cggtgtgggc
    12121 ggcgtacggc tacgccgtgc tgctcggcgt gggctactcc gccaccgcgt cgctcatccc
    12181 ggcgatgctg agcgaccggt tcagcggccg gcacttcggc gcgatcgtcg gcgtgtgcct
    12241 gatcgggagc tcgaccggaa gcgcggtcgg gccgtggctc gcgggtatgc tctacgacgc
    12301 cacgagcagc tacacggccg cgttcctgat cgcggccgcg tgcggcgtga tcgccggcgc
    12361 ggcaggctgg cgcgcccggg cgctgcggat ccgcgcgcct atttcttctc gaagccccga
    12421 accaacgacg cgcgcgccgt agcgccctgc atcagcagcg agaaatccga gccgctgagg
    12481 atgagctgca cgcccatgcc gatgtgccgc tcgaggagct cggccgtgta catgccgccc
    12541 atgccgggga acttgccgtg cttgcggcag gccgcaatca ccttggtgta ggcgtccttc
    12601 accttcgggt cgttgaactg gccggggatg cccatctcga agcagagatc gttggtgccg
    12661 atgaggagcg cgtcgacgcc cttcaccgcg gcgatctcgt ccgcgttctg tacgccttgc
    12721 ggcgattcga tcatcaccac gacgagcgtc tcctcgttga ccatgcgcgc ggcctcgccg
    12781 acggggacgg aggcaaagcc gagctgcgcc agcgcgccgc ccatcgagcg cttgcccacc
    12841 ggcgggtagc ggcagtgatc ggccacgcgc ttcgccgagg ccgcgtcgtc gacatgagga
    12901 aaaacgattc cctgcgcgcc gttgtccagc aggcgcgagg cgtgccagtg ctcgaacccc
    12961 gcgactcgca caatcggcga gacgccggcg gcgagcgcgg cggtagagat ctgcgcagcg
    13021 gtgtcggggt cgagcgagcc gtgctcggcg tcgatgaaca gccaatcgaa gccggcggtc
    13081 ttcgccatgg tggcgatgcc gacggtgcgc gcctggttga cgcccaggcc gatggcgagt
    13141 tttccggcgc gcagctgccg gagagcgtgg ttggggacga tgggcatgcg tgaagactag
    13201 aacatttcgt tacaacacgg ggccgcgttt cgggaaacta ccggcgggat cgctggtctg
    13261 ttagctcaga acgtctcaag gagaaaaatc catgaaggca aaagtggctt atgtggccct
    13321 gcttgcgctc gccattgtcg gaacaggcgt cggcgtatcg cagctgccac tgcagtttcc
    13381 ccatgcaaac gcacaagcac agccggccgc ggtgcagacg ctgccggact tcgctgcgat
    13441 cgtcgaggcg aacaaggcgg cggtggtgaa catcacggcg tccagcgcgg cgaagccgca
    13501 ggcagaggta ccggagctgc cggaggaatt ccgcaacagc ccgttcggcg agttcttccg
    13561 ccgcttccag gggccgcaac cgaacgtgcc gcgccagggg atgggctcgg gcttcatcgt
    13621 cgagtccgac ggcacggtgc tcaccaacgc gcacgtggtc gagggcgccg acgaggtgcg
    13681 cgtgcgcctc accgaccggc gcgagttcaa gggcaaggtg gtcggcgtgg acaagccgag
    13741 cgacatcgcg gtggtgaaga tcgaagccag agggctgccc gtcgtcaggc tgggcgatcc
    13801 ctcgaaagtc cgcgtgggcg aatgggtgct cgcgatcggc tcgccgttcg gcttcgagaa
    13861 cacggtgacc gccggcatcg tgagcgcgac ctcgcgctcg ctgcccgagg gcacttacgt
    13921 accgttcatc cagaccgacg ccgccgtcaa ccccggcaac tcgggcgggc cgctgttcaa
    13981 cctgaagggc gaggtgatcg gcatcgcctc gcagatctac tcgcgcaccg gcggctacca
    14041 ggggctcgcg ttcgcgatcc cgatcgacgt cgcggccaac gtgaaggagc agctcgtgaa
    14101 gcacgggcgc gtcgagcgcg gccgcatcgg cgtggcgatc caggaggtga cgcagtcgct
    14161 cgcccagtcc ttcggcctcg accggccgcg cggcgcgctg gtggcccagg tcgacaacga
    14221 cggccctgcc gcaaagggcg ggatcaaagc cggcgacgtg ctcctcgggg tgaacgggaa
    14281 ggcgatcgac cgctcggccg agctgccgcc gctggtcgcg gcggtgaagc cgggaaccaa
    14341 ggcgagcttc gacgtctggc gcgatggcag gaagcaggtg ctggaagtca ccgtcggcgc
    14401 gctcgagccc gagaaggttg cggccgtggg tccgcagggc gggggcgcgc ctaccggcaa
    14461 gctcgggctc gcggtgcggc cctccgagga cggcctcgtg gtcgagaacg ccaccggcgc
    14521 cgcggcgcga gccgggatcc gccctggcga tgtggttact gcggtaaacg ggcggccggt
    14581 gaagagcgtg gacgacctgc gcgccgcagc cgacaacgcc aagggcacgg tcgcgctgct
    14641 ggtgcggcgg ggcgaagcca gtatttttgt accggtcgac gtcggataac gaaacaaagt
    14701 cagggccgtg acccgatgag cgccgattcc ctgtaggggt aatccctgta gcgctcccgt
    14761 gacacccaaa atccggggcg ggctacagac agggcgcggc aacgccccta gagtgtggct
    14821 tcccccccga gccactcatc ctccctccct tggatagtgg ctcaccccta agaggccgtc
    14881 ttcggacggc ttcttttttt tctgcctgcc tttttgagtt gcttggcctc gcgccgcagc
    14941 ttgttgtgga tcttctgcag gcggatacgg tttcggcgat gcatcgaggt atgtctcatg
    15001 gggcggattc cagtgggcga aaggtcagga tggtaatgcg gcggcggcca gccgcgagtc
    15061 gatgagcgaa gcggcggcgg gcccggcgtt gcgtgcgggg gaatggacgc cgaggatcag
    15121 gaccgcctgc gcgccttgga tgaggagaaa gagctgctcg gcgaggagct cgggctcgct
    15181 cgcgccgctc cgcacgcaga gctcggcaag cttggcgtgc agcttcgcct tgtgcgcctc
    15241 gatcgcctgg cgcgcggggc ggccttgccc gatgatcccg accgcggcgt tggccatgcc
    15301 gcagccgagc gcctccgggt cggtgacgtg ggtggcgagg agcgcgaacg cccggttcag
    15361 ccgcttgcgc ggatcgcccg ggtagcgctg cgacatcgcg tcccagcgct gccagaagcg
    15421 cgcgctgcgt agccgcagcc actcggcgac cagctcgtcc cgcgaggagc cgcgatcgcg
    15481 aatcaggaaa cctcgagcag ctccacttcg aacaccaggg tggcctcggg cgggatcacg
    15541 ccggcgaggc cgtgcgagcc gtaggcgagc gcgggcggga tggtgaggcg ccgcttgccg
    15601 ccgacgcgca tcccggggat gccttcgtcc cagccgcgga tcacgtcgcc cgcgccgagg
    15661 gcgaggtcga gcgggtcgcg gcgcgcccgg ctcgagtcga actgcttgcc gccctgcatc
    15721 cagccgacgt agtgcaccac cacgtcgttt cctgcttcgg cgcgcgcgcc gctgccgacg
    15781 accagatctt ccttgatcag gccgccgggg atgaaatcgt agggttgctg catggaaccg
    15841 cctttccgga atgggccgct attgtacggg cttccagcat gtactggtca gatatacagt
    15901 actggcgcta tagtcgcggc catggcggcg accgcgttcg aaagactgaa ccccaggcag
    15961 cgcgaggcgg cgtgcgccgg ccctgcgccg ctcctcatcg tcgccggggc aggcaccggc
    16021 aagaccaaca ccctcgcgca ccgcgtggcg cacctggttc tgcaaggcgc ttccccggaa
    16081 aaaatcctac tgttgacctt cacccgcagg gccgcgctcg aaatgacgcg ccgcgcgagc
    16141 cgcatcgtgg ccgaggtgag gaaagacgtg cggctcccct ggtcggggac cttccactcg
    16201 gttgccaacc ggttgttgag actctactca aaaagaatct ccctcgcgga aagcttcagc
    16261 gtcctcgacc gcggcgacgc gtcggacctg atggacgtgg tgcgccacga gcgcgggctt
    16321 tccaaatcgg agaagcgctt tccgcgcaag gacacctgcc tcgccatcta ctcccaccgc
    16381 gtcaacaccc aacgcccgct tgccgacacg ctcgcgctca tgttcccgtg gtgcgcggaa
    16441 tgggaagcgg aattgaccgg gcttttccgg aattatgtag agaggaagat tcaaaaccag
    16501 agcctggatt acgacgacct gctgctctac tggcacgcga tgatgcagga cgaagcgctc
    16561 gcggccgaga tcggggtgcg cttcgaccac gtgctggtcg acgagtacca ggacaccaac
    16621 gtgctgcagg cggagatcct gcagcggctc aagccctcgg gcgagggcgt gaccgtggtg
    16681 ggcgacgagg cgcaggcgat ctattcgttc cgcgcggcca cggtcgacaa catccgcaat
    16741 ttccagaaac agggacggac cacggtttcg ctggaagaga actaccgctc gacccagcag
    16801 atcctcgacg cggcgaacgc gctgatcggc aagagcctct attccaggac gaagctcggc
    16861 gcgaagccgc gctacgtgac ggtggcggac gacgaggggc aggcgcagta cgtggtgacg
    16921 cgcgtgctcg aggcgcgcga gcgcggggtg ctcctgagga accaggcggt actgttccgc
    16981 gcctcgcacc actccgacct gctcgagctg gagctcgtga ggcgcaacat tccttacgtg
    17041 aagtacggcg ggctcaagtt cctcgaggcg gcgcacgtga aggacctgct cgcggtgctg
    17101 cgctgggcgg acaacccgag gaaccgggtc gcggcgttcc gggcgctgca gctcctgccc
    17161 ggcgtcgggc cggcgggcgc ggcgcgggtg ttcacgcgct tcgaggccga ggggttctca
    17221 tggaagccgc tcaagaccgg atcggccggg ctcgacgagt tgatgctcta ccttggagaa
    17281 gccccctggg aggggcaggt gcagcgcgtg cgcgagtggt acctgccgca cctcgagcgg
    17341 atctacgacg cagcgcaggt cagggcgggg gatttgttgc aattggaaaa aatctcgggc
    17401 cagttcgcgg atcggtccac cttcctgacc gaaatggccc tcgaccctcc cagcgcgacg
    17461 agcgacctgt caggcgagcc ctacctcgac gaggactacc tcgtgctctc gacggtgcac
    17521 tcggcaaaag ggcaggagtg ggaggcggtg cacatcctca acgtggccga cggcaacttc
    17581 ccctccgagt tctccaccgg ccggcccgag ctgatcgacg aggagcggcg cctgctctac
    17641 gtggcgatga cgcgcgcgaa gag
//
LOCUS       DATBSZ010000076        19549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_762013, whole genome shotgun sequence.
ACCESSION   DATBSZ010000076 DATBSZ010000000
VERSION     DATBSZ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19549
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762013"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..501
                     /locus_tag="VIG70_05080"
     CDS             268..501
                     /locus_tag="VIG70_05080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8249960.1"
                     /translation="MRDGERLAAMSYTVAGTRVIIDHTDVDERLRGTGAGKKLVAAAV
                     EWARKENARLMPLCPFAKSVFDKTPEYSDVLAK"
     gene            543..1565
                     /locus_tag="VIG70_05085"
     CDS             543..1565
                     /locus_tag="VIG70_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006046617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8249961.1"
                     /translation="MASDEQLQDWRSRALRVEEEVGKAIVGQQGTARLISVALFARGH
                     VLLEGDVGIGKTTILRAFSRAIGGGFERVEGTVDLMPGDLIYHTYVDAEGKPRIEPGP
                     LLRHGEHLVTFFFNEINRARPQVQSLLLRAMAERTVSAFNREYRFPHMLVFADRNRVE
                     KEETFELPSAARDRFMFELRMPTPADAAVRRALVFNPDFHDVDELLQRVAPGVLPWQE
                     LERIAAAIQRSVTASPALERYVLDLWEASQSPQKFGVRLDGVDMDKLILAGASPRGMM
                     TLARAARVVAWLAGRTYLVPDDVQSLAPSVLRHRVFFTPIYELRRAQIAEALVDRILE
                     RVPTPR"
     gene            1570..2424
                     /locus_tag="VIG70_05090"
     CDS             1570..2424
                     /locus_tag="VIG70_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8249962.1"
                     /translation="MSPPREFHYRLPDRVAGWRPGAHPGTSLGAGQEFVSHMSLHERP
                     DPRRLDLRASLRDLRGDWLVRVHRQRAGACVHAVVDVSASMGFGSHKTKLQVAADFVE
                     ALGQSAFRVGDALGMIAFDATERTDLFVPPLVRRGMGAVMASMLRQCACAAGGFEGLA
                     DAAQRAAGRRGLVFLVSDFHWPLERLAAALDLLAHARVVPIVVWDPAEIEPPMQDAIA
                     PLRDAESGVRRTLWVRPKLRGQWRDAVARRRLELARFLAAHAIRPFYVSGAFDAEAMS
                     QYFLEAAA"
     gene            2421..3278
                     /locus_tag="VIG70_05095"
     CDS             2421..3278
                     /locus_tag="VIG70_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium incorporation protein MxaA"
                     /protein_id="HEY8249963.1"
                     /translation="MRHTIAALALAAGATSVAAQPEAVVEQPRAFGYVLGDVLTQRVL
                     LQLEGHEFAPPALPKAERVGVWLERRAARIESRPDGRRWLAVDYQLINAPQALTTVNL
                     PALELQDKSAGVSLKIAAWPISVNALTPRAAFGAGGLDELRPDRAAPMIATQPIRRRI
                     AIWSGAFLLALAAWLGWVAWRNWRAASARPFATALREMRRLDETAPAAWQALHRAFDQ
                     AAGRVTQAATLPALFERAPHLRPLRAQIEQFFAQSGELFFGRGSPADRISVRALCARL
                     RRIEKRHER"
     gene            3275..4291
                     /locus_tag="VIG70_05100"
     CDS             3275..4291
                     /locus_tag="VIG70_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8249964.1"
                     /translation="MSGGVDFAQPLALLLLPLALLPLSRKRRDTLAFSHLAWLPRDRL
                     GQIAGFLWRALAVLAIGSMVIALAGPGRPETQVTRTGYGAEILLLIDRSRSMDERMLP
                     SDWRTIDPLSLIHQVRSRGPIKAQVARELLAEFVHQRPSDRFAVMFFSTNPLNVVPFT
                     QHDAVVLAGIEAGGVGRGLGDTDVGNAMSAAIGEFERRPYSGSRIILLVSDGGAQLDD
                     ATRRRLRAGLLRSRIALYWIYVRSFNGPALDTQDRGSEALPEIALHRFFQALATPYQA
                     YQAEIPEDLAKAVADVGKQQNLPLDFVEQVPRQDYSRHFVAAAALACLLLLAYRSLLL
                     RSWA"
     gene            4288..4812
                     /locus_tag="VIG70_05105"
     CDS             4288..4812
                     /locus_tag="VIG70_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MxaK protein"
                     /protein_id="HEY8249965.1"
                     /translation="MRQRTIHFAFAAAAIGFGSIAAYHGLRLHRASQVNAAIATASVS
                     GSEAGVAEAQFARALASSESGDSAAALQLYKALIQGGRADLKRAALYNLGNLHLREAM
                     KSDEAARSLPLIELAKQSYRDLLRIDPADWDARYNLERALWLAPEMEEAAVNEPPAPT
                     RRAISTAPGRSELP"
     gene            4809..5705
                     /locus_tag="VIG70_05110"
     CDS             4809..5705
                     /locus_tag="VIG70_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxaL protein"
                     /protein_id="HEY8249966.1"
                     /translation="MSLRALVAGAVDKQSRAILSALALLLCAWAMPAFNWPRATYDYI
                     VVFDITQSMNVEDYRIGDSPVSRFAYAREATRRALRDLPCGSRVGWGAFAEYRTLLLL
                     APIEVCAHYHDLLASLDKIEGQMRWGNASEVRKGVFWSIRAAKEASGHPNVIFLSDGH
                     EAPPLAAEGRAGFDDVKPGDIRGWLIGVGGYTPRPIPRTDDQGRLVGYWRAEDVVQIA
                     GSDGDAAAEREHLSALRESHLRTLAQQVGFEYARLAGPESISEAMRDPRFARARPVPI
                     DLYWLPVAAALLLLAIRFRPGS"
     gene            complement(5714..6355)
                     /locus_tag="VIG70_05115"
     CDS             complement(5714..6355)
                     /locus_tag="VIG70_05115"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEY8249967.1"
                     /translation="MAAPKITCAPSGPYLLADGSGVRRASGETWPAAASVALCRCGGS
                     KNKPFCDGTHGKSGFRDDNTADPAKNKRKAYAGKGITIFDNRAICAHAAFCTDELKSV
                     FRHHDEPWIDPDGAQADEIIRTIRKCPSGALSYAINGVEAQPPQRPPMVTVTDNGPYA
                     VTGGIELVGVKLGAGASLEHYTLCRCGASKNKPFCDGSHWSLPAGWGQLADPP"
     gene            complement(6357..7313)
                     /locus_tag="VIG70_05120"
     CDS             complement(6357..7313)
                     /locus_tag="VIG70_05120"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249968.1"
                     /translation="MKIITAFFCGMALSTAAFAQTWPAKPIKLILGYPTGGATDAMAR
                     PLIAKMEPMLKQPLIIEYRPGAGATAGAIAVAGAAPDGYTLHLIDAGPATIVPNVQKL
                     PYDPLTSFTPIGLICAGGTAIVAHPSVPANSLAELLKLAKSQPGKLSYGTSGVAGAGH
                     MAAELLQIATQVQMVHIAYKGGGPAMTDLLGGHVPVLFSSMATAVPHVKSGKIKGLAV
                     TTPARVPAISDVPTVAELGFPGFEALIWFGLVGPAKLPPEITATMSRALVGALEDKSV
                     QDAIRNLGYEPVPNTPAQFAAIIRDDLEKWRKVVQEAKIKVD"
     gene            complement(7310..8179)
                     /locus_tag="VIG70_05125"
     CDS             complement(7310..8179)
                     /locus_tag="VIG70_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY8249969.1"
                     /translation="MKPLERIAYSPITQRKPLRLPGGARMVVWTIVNVEEWDIDQAMP
                     RTVLTPPAGGSPMPDIPNWAWHEYGNRVGFWRLAEILDSLSIKAALAINGTAVRAYQP
                     ISRAALERGWEFMGHGYTQRNMQKVENEREDIARTTEAIRAFTGKRPRGWLGPGLTET
                     WDTPDLLVEAGYEYVADWVLDDQPVVLKTRSKPIVNIPYTQECNDVAMMLIQHHEAGQ
                     YYQRAIDQFEQIYADSRDSARVMALVVHPYIMGVPHRAKYYRRALEGIRKHKEVLFWT
                     GEQILDWYLEETK"
     gene            8310..9170
                     /locus_tag="VIG70_05130"
     CDS             8310..9170
                     /locus_tag="VIG70_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8249970.1"
                     /translation="MDAKFWLIQTFNGVSYGALLFLLASGLTLIFGVMRIVNLSHGAY
                     FLLGGYVALTVIKATGSWLLALPLAGLAIALIGLLVERIFLRPLGFDPLRQVLLTVGF
                     AFLFQQAALDIWGGDNLIIQPPPALSKSVVIGGLHLPLYRIFMIALAAAIGLALWFAM
                     ERTRMGAMVRATVDDAEMARGVGIDTSRVSMFIFALGAFLAALGGVIGGAFLGVYPGL
                     DFEVLPIAFAVVIIGGMGSLAGAAIGSLLVGLADNFGKALFPEVAYFSLYAPMVLILA
                     MKPTGLLGRE"
     gene            9167..10096
                     /locus_tag="VIG70_05135"
     CDS             9167..10096
                     /locus_tag="VIG70_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8249971.1"
                     /translation="MKQILVLLGILGIVSVLAPFAGPFVILLLAQVLVLGIMAMSVDL
                     LLGYTGLPSLGQAAYLGVGAYLTAILATKYGIGLGWDFWMVVALGMLSGAATAAFFGL
                     FAIRASGVYFLMITLALGMCVWGLAYRWNSLTGGDNGINMRGRPKLGELAFSNDLTFF
                     YVVLGFFVASLFALHLLVSSPFGKSLVGIRERELRMRILGYNTWLHKYIAFVIAGAFG
                     GLAGVLWAHLNGIVSPSDVILTTSVDALLMVVLGGPGTLVGGLIGATVVVFLREYLAT
                     LVQWWQYVLGAVYVLTILYLPGGIMALFKGGRR"
     gene            10093..11322
                     /locus_tag="VIG70_05140"
     CDS             10093..11322
                     /locus_tag="VIG70_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8249972.1"
                     /translation="MKRFTLILAGVFAASPAYAQELKIGFLAPMTGIFAQIGKDMENG
                     FQMYLDEQKSNFAGARVTFIVEDEEGKPPVSVRKAEKLARQDNVHMLVGGLLASTGYA
                     IAPVSTRLKTVYIPSIPAADDLTQRDLKDYPYLVRTGWTSSQPHHPLGRWACDNKYKR
                     VVAIAADYAFGHEVVGGFQKVFEDCGGQIVQKIWPPLGTKDFGPYIPTIKQDADAIFS
                     LMVGPMALQFPKQLQESGVKKPIIGGGTSYDEFVLPSMGDEVLGHVSALQYSAAIDTP
                     KNREFVRKYREKYGKVPSYYSESNYTTAQMIHEVMKQTKGKWPGPEKFVAMMAGLKID
                     AVRGPVRFDEMRNPVQNIYIKKVEKKKMHGYDKDELWNSVIQTYPNVGQFWTYKKEEF
                     LKQPVYSRDFPPCKYCN"
     gene            11304..12044
                     /locus_tag="VIG70_05145"
     CDS             11304..12044
                     /locus_tag="VIG70_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249973.1"
                     /translation="MQVLQLKGLSKSFGGLAAVREVTLEVHAGERKAIIGPNGAGKTT
                     LFNLITGVFPCSSGSIVLFEKDVTRWPSHRRTALGMARTFQITSLFPKLTVLDNVLLA
                     LAGVGWTKLVMWKPLASYEKFKTRARQLLEAAGYWDRRDVEIRNLSHGEQRQIEVVLG
                     LASDPKLLLLDEPAAGLSSGESREMAEFLKRLDPSLAILLIEHDMDVVFDVVSDISVL
                     HFGAVVESGSCTQIQKSERVQQIYLGTG"
     gene            12041..12733
                     /locus_tag="VIG70_05150"
     CDS             12041..12733
                     /locus_tag="VIG70_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8249974.1"
                     /translation="MILQVDDIHTYYGDAYVLQGLSLSLEKGQILGLLGRNGVGKTTL
                     VNSIVGFNRPRRGRIVFKGADITRLSSFETIRNGMGLVPQGRRVFASLTVEENLKVAR
                     SAEGAWTLPRVYALFPRLEERRRQRAKTLSGGEQQMLAIARGLMTNPDCLIMDEPSEG
                     LAPIIIQGLWQAIAKLKAQGLSILLVEQNAALALQLVDYVHVISKGRVVYSGAPKALW
                     ADEDVKSRYLGI"
     gene            complement(12740..13726)
                     /locus_tag="VIG70_05155"
     CDS             complement(12740..13726)
                     /locus_tag="VIG70_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249975.1"
                     /translation="MSYPNDLAVLYEHPEWQKPLFAALERRGIRYAAVDLKRAAFADA
                     DEPLAPLYFNQASPSAYVRGNVRAVPYALAHMRALQIRGVRVLNGADAFALEISKTAQ
                     AALLRNLGVPAPRVWTFNHLEALRARAHEIAFPVLLKPEQGGSGARILLVESFQQLEA
                     FLNQHPDHWLPDNLLLLQEYLDTDPAKGIVRMEFLGGELLYAMRVISHGRFNLCPSPV
                     CNSETASCEVENTVPPAEFYPYKDVPAQAVAWGKRIVAAGKLDVAGIEYLETRDGRLV
                     FYDVNANSNLRPSVAQAFGFDPFERVVDFLAREIRAYEGFLRFAGLRGRIEV"
     gene            13909..14376
                     /locus_tag="VIG70_05160"
     CDS             13909..14376
                     /locus_tag="VIG70_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY8249976.1"
                     /translation="MRASLAIAILALAGCSSTPQKAPVAGSGSTRPTADIAGQAAGYA
                     QTMIGKPYRYGGTSPKGFDCSGLVFYSYKQAGVALPHSTEKQRSAARSIKMAELRRGD
                     LLFFNQEGKKYGHVAIYVGDGRFVHAPSSGKSVRSDRLDTPYWKKHLSEARRI"
     gene            14507..15616
                     /locus_tag="VIG70_05165"
     CDS             14507..15616
                     /locus_tag="VIG70_05165"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY8249977.1"
                     /translation="MQKTSVRFLTALLGLLVSAQVAAATFVYVSNAEDGDIGLYTLRP
                     DGSLQPGQRFKAGPMVMPMSVSPDKRFLVAAVRSKPFSAHTYSIDRGTGALKPIGSGP
                     LAESFPYIAFDRTGRHLLGASYGANLVSVNPVNPDGTIGAPAQVIPTARNAHAIITDR
                     TNRFVFVPHLGTDQVFQFQFDEKSGRLAANTPPVLQLKAGSGPRHLITSPDNRFVYLL
                     NELTATVSTLSLENGLLKEVSSASALPPESKLGPGMPRPQQGRDVSNDIWASDLHLTP
                     DGRFLYAAERTGSTINGFSVDSASGKLTYLGSTPTEKQPRGFRIDPTGHFMVVSGEKS
                     ETISSYRIESNGLLVPVGRVATGKGSNWVEIVSFD"
     gene            15666..16448
                     /locus_tag="VIG70_05170"
     CDS             15666..16448
                     /locus_tag="VIG70_05170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8249978.1"
                     /translation="MPASRLFAAFLLAAFSALAFAQAQKAPAAYEPTVGQEGKDVVWV
                     PTPQAVVDKMLDMAKVTSKDFVMDLGSGDGRTVITAAKRGARAVGVEYNPDMVELSKH
                     NAEKEGVAGNATFMKADLFETDFSQATVITMFLLPDINIKLRPKILSLKPGTRIVSNS
                     FTMGDWKADETAELNSSQGCNNSWCTALLWIVPAKVGGTHKVPQGELMLKQEFQMLSG
                     TLRADGKTYAVTGKVNGEEVVFKAGGREYHARMNGKQLELHK"
     gene            16445..17410
                     /locus_tag="VIG70_05175"
     CDS             16445..17410
                     /locus_tag="VIG70_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8249979.1"
                     /translation="MRRLCGLLLAAALALPGAAAAAYPDKPISLVVAYSPGGGTDLIA
                     RQIAPFLEKYLGGGARIIIVHRPGAGGEIGFAAIANAAPDGYTIGFVNTPPLITVPIE
                     RTAQFGGPQRFELLGNVIDDPCNFTVHADSEIRDLKGLAAYAKANPGKVTVGSSGIGS
                     DDHLLMLMFERAAGVKMTHIPFKGAADVRTAVLAKQIMVGAINIGEAVQSIKGGAPLR
                     NIGQFSPARTTVAPELPTAREQGYDIELSSLRGLAAPRGLPADVRERLVKAVADAAND
                     PEFQAKATSVFAPLRFLTPAQFDAVIREAEVGFKQLWKELPWGDK"
     gene            complement(17482..18093)
                     /locus_tag="VIG70_05180"
     CDS             complement(17482..18093)
                     /locus_tag="VIG70_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8249980.1"
                     /translation="MFERLHHIAYRCRDAQRTVDFYTKVVGLKYCAGLLPPESRSNPS
                     WPLNEPGRPPRKVVGAPADSIHIFFELGDGSYLAFFDVASAPEEAPEITPWWVKHIAF
                     ETESMEKLLAAKARLESHGVQVLGPKDHDFCQSIYFMDPDGHRLEITVRTEQPDSWDK
                     LAAEAPRMLAQWNERKRRKFGKSTEPLPKKLAAMPEDSTIEPA"
     gene            18179..18955
                     /locus_tag="VIG70_05185"
     CDS             18179..18955
                     /locus_tag="VIG70_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8249981.1"
                     /translation="MSLVSERQNGAGAAARLEPFAGHWVVEGEVQKSAYGPASRWTSD
                     ERCEWLPGQRFLVNRWDARVGERDFRGMAVFGYDPQRGYFARFHDHAGNAPDYRMNID
                     GPTWTLAGDAQRAVYEFGTGTIAIRWEWRDDNGWHSLRTLRARRGVSASEMVHEYFAA
                     FQAQDRATAERLLAQDFVFSSPRDDRIDRAAYFERCWPNASRLRAFWMERLFEHDGEV
                     FVRYSAERLADGVRFRNTEIIRVARGQIREIDVYFGRDLR"
     gene            complement(19022..>19549)
                     /locus_tag="VIG70_05190"
     CDS             complement(19022..>19549)
                     /locus_tag="VIG70_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY8249982.1"
                     /translation="MLTLGIPGSATTAIMLGGLMMWGLRPGPMLFEKNPQFVWGLIAS
                     QYIANILLLILSTLCIPLFVRAVRIPYSILMPLIVVFCVTGAYSLKNNLWDVGQMLVF
                     GVIGYFMKKLGYSPAALVLALVLGPLAERALRQSLIISDAGLGILFMRPISGVLTVLA
                     LLAVAVPVVRAFLRK"
BASE COUNT         3246 a         6534 c         6501 g         3268 t
ORIGIN      
        1 gcggcaacca gaaccagctg tgcgacgagc agcaggactt tcatggttcc ccctccggag
       61 acggcctgcc tgccatggta cgccgtagtg cgcctggcgg ataatgggcg cctccccgga
      121 gggtcccatg gcaaaccgcg agcagcgcag caaccgagag aagaagaagc ccaagcagcc
      181 caacaagccg tcgccgacgc ccgggacgcc gcaaccctgg aagaaataac gcacgagcgc
      241 tcgggccacc gcggcgcgtt cgtctggatg cgcgacggcg agcgcctcgc ggcgatgagc
      301 tacacggttg ccggcacccg ggtgatcatc gaccacaccg atgtggacga gcggctgcgc
      361 ggcaccggcg ccggcaagaa gctggtcgcg gcggcggtgg aatgggcgcg caaggaaaac
      421 gcgcgcctga tgccgctctg tccgttcgcc aagtcagtgt tcgacaagac gccggaatat
      481 tccgacgtgc ttgcgaagta ggatctttga agcccgcctc cccacgaaaa agggagcacg
      541 cgatcgcctc cgacgagcaa ctccaagact ggcgaagtcg cgcactgcgc gtggaggagg
      601 aggtggggaa agcgatcgtc ggccagcagg gcacggcgcg gctgatcagc gtcgcgctgt
      661 ttgcccgcgg acacgtgctg ctcgaaggcg atgtcggcat cggcaagacc acgatcctgc
      721 gcgctttctc gcgcgccatc ggcggcggct tcgaacgcgt cgaaggcacc gtcgacctga
      781 tgcccggcga tctcatctat cacacgtacg tggacgcgga aggcaagccg cgcatcgaac
      841 cgggtccgct gttgcgccac ggcgagcacc tggtgacgtt cttcttcaac gagatcaacc
      901 gtgcgcggcc gcaggtgcaa tcgctgctgc tgcgcgcgat ggccgaacgc accgtttccg
      961 ccttcaatcg cgaataccgc ttcccgcaca tgctggtgtt cgccgaccgc aaccgggtcg
     1021 agaaggagga gaccttcgag ctgccgtccg cggcgcgcga ccgcttcatg ttcgagctgc
     1081 ggatgccgac gccggcggat gccgcggtcc ggcgcgcgct ggtattcaac cccgacttcc
     1141 atgacgtgga cgagctgctg caacgcgtcg cgcccggcgt gctgccgtgg caggagctcg
     1201 agcgcatcgc tgccgccatt caacgcagcg tcaccgcgag cccggcgctg gagcgttacg
     1261 tgctggatct gtgggaggcg agccagtcgc cgcagaaatt cggcgtgcgc ctcgacggcg
     1321 tcgacatgga caagctgatt ctcgctggcg ccagcccgcg cggcatgatg acgctcgcgc
     1381 gcgcggcgcg cgtcgtcgcc tggctcgccg gccgcaccta tctcgtcccg gatgacgtcc
     1441 agagcctcgc gccttcggtc ctgcgacacc gcgtgttctt cacgccgatc tacgagctgc
     1501 gccgcgcgca gattgccgag gcgctcgtcg accggatcct ggagcgagtc cctaccccgc
     1561 gctagcgaca tgagcccgcc ccgggagttt cactaccgtc tgcccgaccg ggtcgcgggc
     1621 tggcggcccg gcgcacatcc cggaacaagc ctcggcgcgg gacaggaatt cgtctcgcac
     1681 atgagtctcc acgagcggcc ggatccgcgc cggctcgact tgcgcgcgag cctgcgcgat
     1741 ttgcgcgggg actggctggt gcgcgtacat cgccagcgcg ccggggcgtg cgtccacgcc
     1801 gtggtcgatg tctcggcttc catgggcttc ggctcgcaca agaccaaact gcaggttgcg
     1861 gcggacttcg tggaagcgct cggccaaagc gccttccgcg tcggcgacgc gctcgggatg
     1921 atcgctttcg atgccacgga gcgcaccgat ctgttcgtgc cgccgctcgt gaggcgcggt
     1981 atgggcgcgg tcatggcctc catgctgcgg caatgcgcct gcgccgccgg gggcttcgag
     2041 ggactcgcag atgccgcgca gcgcgccgcg gggcggcgcg gcctggtatt tctcgtttcc
     2101 gacttccatt ggccgctcga acggctggcg gccgcgctcg acttgctcgc gcacgcccgc
     2161 gtcgtgccga tcgtggtctg ggacccggcc gaaatcgagc cgccgatgca ggatgcgatc
     2221 gcgccgctgc gcgacgccga gtccggcgtg cgccgcacgt tgtgggtgcg cccgaagttg
     2281 cgcggccagt ggcgcgatgc ggtggcgcgc cggcggctgg agctcgctcg attccttgct
     2341 gcgcatgcga tccgtccgtt ctacgtgagc ggcgcattcg acgccgaggc catgtcgcag
     2401 tacttcctgg aggcggccgc atgaggcata ccatcgctgc gctcgccctt gcggcaggcg
     2461 cgaccagcgt cgccgcccag cccgaagccg tcgtcgagca accgcgcgcc ttcggctacg
     2521 tcctgggcga tgtgctgaca cagcgcgtgc ttctgcagct cgagggacac gaattcgcgc
     2581 cgcccgcgct ccccaaggcc gagcgggtcg gcgtttggct ggagcggcgc gcggcgagga
     2641 tcgagtcgcg ccccgacgga cgtcgctggc tggccgtcga ctatcaactg atcaacgccc
     2701 cgcaagcgct gacgacggtg aacctgccgg ccctggagct gcaggacaaa tccgccgggg
     2761 tgagcctcaa gattgccgcc tggccgatca gcgtgaacgc gctcacgccg cgcgctgcgt
     2821 tcggcgcagg agggctggac gagctgaggc ctgaccgcgc tgcgccgatg atcgcgacgc
     2881 agccgatacg acgtcgcatc gcaatctggt cgggcgcgtt cctgctcgcg ctggccgcct
     2941 ggctcggctg ggtcgcatgg cgcaactggc gcgcggcttc cgcgcggccg tttgcaacag
     3001 cgctgcgcga gatgcgtcgt ctcgatgaga cggcgccggc agcatggcag gcgttgcatc
     3061 gtgccttcga tcaggcggcc ggccgcgtca cgcaggccgc gacgctcccg gcgctgttcg
     3121 agcgggcgcc gcatttgcgc ccgctgcgtg cgcagatcga gcagttcttc gctcaatcgg
     3181 gcgagctgtt cttcggccgc ggctcgccgg ccgatcgcat ctcggttcgt gccctctgcg
     3241 cccggctgcg gcgcatcgaa aaacgccacg aacgatgagc ggcggcgtcg atttcgccca
     3301 gcccctcgcc ctgctgctgc tgccgctcgc cttgctgccg ctctcacgca aacggcgcga
     3361 taccctggcc ttctcgcacc tcgcttggtt gccgcgcgac cgcctcggtc aaatcgccgg
     3421 cttcctttgg cgcgccctgg cggtgctcgc catcggcagt atggtcatcg cgctggcggg
     3481 cccaggccgg cccgaaacgc aggttacgcg caccgggtac ggcgcagaga tcctgctgct
     3541 cattgatcgc agccgcagca tggacgagcg gatgctgccg agcgactggc gcacgatcga
     3601 tccgctttcc ctgatccacc aggtgcgctc gcgcggcccg atcaaggccc aggttgcacg
     3661 cgagctgctc gcagaattcg tgcaccagcg tccgagcgat cggttcgcgg tgatgttctt
     3721 cagcaccaat ccgctgaacg tcgttccgtt cacgcaacac gacgcggtcg ttctggccgg
     3781 gatcgaggcg ggcggcgtgg gccgcggcct gggcgacacc gacgtcggca atgcgatgag
     3841 tgcggcgatc ggggaattcg agcggcgccc gtactccggc agccggatca tcctgctcgt
     3901 gtcggatggc ggcgcccagc tggatgacgc aacgcgccgt cgcctgcggg ccggcctgtt
     3961 gcgcagccgc attgcgctgt actggatcta cgtcagatcc ttcaacggtc cggcgctcga
     4021 tacgcaagat cgaggcagcg aagctctgcc ggaaatcgcg ctgcaccggt tttttcaggc
     4081 cctggcgacg ccgtatcaag cctatcaggc ggagatcccc gaggatctgg cgaaggccgt
     4141 tgccgacgtc ggaaagcagc agaatctgcc gctcgatttt gtcgagcagg tcccgcggca
     4201 ggactacagc cggcacttcg tggcggccgc cgcgcttgcc tgcctgctgc tgctcgccta
     4261 tcgatcgctg ctgctcagga gctgggcgtg aggcagcgca cgattcactt cgcgttcgct
     4321 gcagcggcaa tcggctttgg ctcgatcgct gcttatcacg gcttgcgcct gcaccgcgcc
     4381 agccaggtca acgcggccat cgccacggca agcgtttcgg gctccgaggc tggcgtggcc
     4441 gaagcgcagt ttgcgcgcgc gctcgcctcg tccgagtcgg gcgactccgc ggcggcgttg
     4501 cagctctaca aggcgctgat ccagggcggc cgcgccgacc tgaagcgggc tgcgctctac
     4561 aacctgggca acctgcatct gcgcgaagcc atgaagagcg atgaggcggc gagatcgctg
     4621 ccgctcatcg agctggccaa acagagctat cgggacctgc tgcgcatcga cccggccgat
     4681 tgggacgcgc gctacaacct cgaacgcgcg ctatggctcg ctccggaaat ggaagaggcg
     4741 gctgtcaacg agccgccggc accgacgcga cgcgcgatca gcactgcgcc gggcagaagc
     4801 gagctgccgt gagcttaaga gcgctggtcg cgggcgcagt cgacaagcag tcgcgcgcga
     4861 tcttgtcggc actggcgctg ctgctctgcg catgggccat gccggccttc aactggccgc
     4921 gcgcgaccta tgactacatc gtggtcttcg acatcacgca gagcatgaac gtcgaggact
     4981 acaggatcgg cgattcgccc gtaagccgct tcgcgtatgc gcgtgaggcc acgcggcggg
     5041 cgctgcgcga tctgccgtgc ggctcccggg tcgggtgggg agccttcgca gagtaccgca
     5101 cgcttctcct gctcgcgccg atcgaggtgt gcgcgcatta ccacgacctg ctcgcatcgc
     5161 tcgacaagat cgagggccaa atgcgctggg gcaacgcgag cgaagtgcgc aagggggtgt
     5221 tctggtcgat ccgcgcggcg aaggaagcga gcggccatcc gaacgtcatc ttcctgagcg
     5281 acgggcacga agcgcctccg ctcgccgcag agggtcgcgc ggggttcgac gacgtgaagc
     5341 ccggcgacat ccgcggatgg ctgatcggcg tcggcggcta cacacccagg ccgattccca
     5401 ggaccgacga ccagggcagg ctcgtcggct attggcgcgc cgaagatgtc gttcagatcg
     5461 ccggcagcga tggcgatgct gcggcagagc gcgagcatct gtcggcgttg cgcgaaagcc
     5521 acctccgcac cctcgcgcag caggtcggct tcgagtacgc acgcctggcc ggacccgagt
     5581 cgattagcga agccatgcgc gatccacggt tcgcccgggc gaggccggtc cccatcgatc
     5641 tctattggct gccggtggct gccgcgctac ttctgctggc catacgcttt cgccctggga
     5701 gttagataga gagtcacggc ggatcagcga gttggcccca cccggctggt aggctccaat
     5761 ggctcccgtc gcagaacggc ttgttcttcg acgcgccgca gcggcacagc gtgtaatgct
     5821 ccagggaggc gccggcgccg agcttcactc ctacgagctc gatgccgccc gtcaccgcgt
     5881 acgggccgtt gtcggtcacc gtcaccatcg gcggccgctg cggcggctgc gcctcgacgc
     5941 cgttgatggc gtagctcagc gcgccggagg ggcatttccg aatcgtcctg attatttcgt
     6001 cggcctgcgc tccgtccgga tcgatccacg gctcgtcgtg gtgacgaaac acggatttca
     6061 gctcgtcggt gcagaacgcc gcgtgcgcac agatggcgcg gttgtcgaag atggtgatgc
     6121 ccttgccggc gtaggccttc ctcttgttct tggcaggatc ggcggtgttg tcgtcccgga
     6181 agccgctctt gccgtgggtg ccgtcgcaga acggcttgtt cttcgagccg ccgcagcggc
     6241 acaacgcgac gcttgcagcc gccggccagg tttccccgga ggcacggcgc acgcccgatc
     6301 catcggcgag cagatacgga ccgctcggcg cgcaggtgat cttcggcgcg gccacctcaa
     6361 tccaccttga tcttcgcctc ctgcaccacc ttgcgccatt tttcgaggtc gtcgcggatg
     6421 atggcggcga actgcgcggg cgtgttgggc acaggctcgt agccgagatt gcggatcgcg
     6481 tcctgcacgc tcttgtcctc gagcgcgccc acgagcgctc ggctcatggt ggcggtgatt
     6541 tccggcggca gcttggccgg cccgaccagg ccgaaccaga tcagcgcctc gaagccggga
     6601 aatccgagct cggccacggt gggcacgtcg gagatcgcgg gcacgcgcgc cggcgtggtg
     6661 accgcgaggc ccttgatctt cccggatttc acgtgcggca ccgcggtcgc catcgaggag
     6721 aacagcaccg gcacgtggcc gccgaggagg tcggtcatcg ccggcccgcc gcctttgtag
     6781 gcgatgtgca ccatctgcac ctgcgtggcg atctgcagca gctccgccgc catgtgcccg
     6841 gcgccggcga cgcccgaggt gccgtagctc agcttgccgg gctgcgattt cgcgagcttg
     6901 agcagctcgg cgagggaatt cgccggcacc gaagggtgcg cgacgatcgc cgtgccgccg
     6961 gcgcagatga gcccgatcgg cgtgaagctg gtgagcggat cgtaaggaag cttctgcacg
     7021 ttcggcacga tagtcgccgg gccggcgtcg atgaggtgca gcgtgtagcc atcgggcgcc
     7081 gcgcccgcca cggcgatggc gccggcagtg gcgcccgccc cgggacggta ttcgatgatc
     7141 aacggctgct tcagcatcgg ctccatcttg gcgatgagcg gccgcgccat tgcgtcggtc
     7201 gcgccgccgg tgggatagcc gaggatcagc ttgatcggct tggcgggcca ggtctgggcg
     7261 aatgccgcgg tcgaaagtgc catgccgcag aaaaaggcgg tgatgatctt catttggtct
     7321 cctccagata ccaatcgagt atctgctctc cggtccaaaa gagtacctct ttgtgcttgc
     7381 ggatgccttc cagcgcgcgc cggtagtact tggcgcggtg cggcacgccc atgatgtagg
     7441 gatgcactac gagcgccatc acgcgcgcag agtcgcgaga gtcggcgtag atctgctcga
     7501 actgatcgat tgcgcgctgg tagtactggc cggcctcatg gtgctggatc agcatcatcg
     7561 cgacatcgtt gcattcctgg gtgtacggaa tattgacgat cggcttcgag cgcgtcttga
     7621 gcaccaccgg ctggtcgtcg agcacccagt cggcgacgta ctcgtagccc gcctccacca
     7681 ggaggtcggg cgtgtcccag gtctcggtga gccccggccc cagccagccg cgcgggcgct
     7741 tgccggtgaa cgcgcggatc gcctcggtag tcctggcgat gtcctcgcgc tcgttctcga
     7801 ccttctgcat gttgcgctgc gtatagccgt ggcccatgaa ctcccagccg cgctcgagcg
     7861 ccgcccgcga gatcggctgg tacgcccgta ccgcggtgcc gttgatcgcc agcgcggcct
     7921 tgatgctcaa ggaatccagg atttcggcaa gccgccagaa accgacgcgg ttgccgtatt
     7981 cgtgccacgc ccagttcggg atgtcgggca tcggcgagcc gcccgcgggc ggcgtgagca
     8041 cggtgcgcgg catcgcctgg tcgatgtccc attcctcgac gttgacgatg gtccagacca
     8101 ccatgcgcgc gccgccggga aggcgcaaag gcttgcgctg ggtgatgggc gaataggcga
     8161 tgcgctcgag tggcttcata gagcgaatgt aacgacaaac caaaatcagg gacagaccac
     8221 ggtttgatgc cggttccact gagcgaaaac ggggtctgtc cctgattttg agcctgtaat
     8281 ccgttactct tgcgcccgga ggaggaggcg tggacgcgaa gttctggctg atccagacct
     8341 tcaacggcgt gtcgtacggc gcgctgctgt tcctgctcgc aagcggcctc accctcatct
     8401 tcggcgtcat gcggatcgtg aacctttcgc acggcgcgta cttcctgctc ggcggctatg
     8461 tcgcgctgac ggtgatcaag gcgaccggct cctggcttct tgcattgccg ctggcaggcc
     8521 tcgcaatcgc cttgattggc ctcctcgtcg agagaatctt cctcaggccc cttggcttcg
     8581 atccgctgcg ccaggtgctg ctcacggtgg gcttcgcgtt cctgttccag caggcggcgc
     8641 tcgacatctg gggcggcgac aacctgatca tccagccgcc gccggcgctt tcgaagagcg
     8701 tcgtgatcgg cggcctgcac cttccgctgt accgcatctt catgatcgcg ctcgccgccg
     8761 cgatcgggct cgcgctctgg ttcgcgatgg agcgcacgcg catgggcgcc atggtgcgcg
     8821 cgaccgtgga cgacgcggag atggcgcgcg gcgtgggcat cgacacctcg cgcgtgtcga
     8881 tgttcatctt cgcgctcggc gccttcctcg ccgcgctcgg cggcgtgatc ggcggcgcgt
     8941 tcctcggcgt ctaccccggc ctcgacttcg aggtgctgcc gatcgcgttc gcggtggtga
     9001 tcatcggcgg catgggcagc ctcgccggcg ccgccatcgg cagcctgctg gtcggcctcg
     9061 cggacaattt cggcaaggcg ctgtttccgg aggtcgcgta cttcagcctg tacgcgccga
     9121 tggtcctgat cctcgcgatg aagcccaccg gattgctggg ccgggaatga agcagatcct
     9181 cgtccttctc ggcattctgg gaatcgtctc ggtgctggct cccttcgccg gcccgttcgt
     9241 catcctgctg ctcgcgcagg tgctggtgct cggcatcatg gcgatgagcg tcgacctgct
     9301 gctcggctac accggcctgc cgtcgctcgg gcaggcggcg tacctcggcg tcggcgcgta
     9361 cctcaccgcg atcctcgcca ccaagtacgg catcggcctg ggatgggact tctggatggt
     9421 cgtggcgctc gggatgctga gcggcgccgc caccgcggcg ttcttcggcc tgttcgcgat
     9481 ccgcgcgagc ggggtgtact tcctgatgat cacgcttgcg ctcggcatgt gcgtctgggg
     9541 cctcgcctac cgctggaact cgctcaccgg cggcgacaac ggcatcaaca tgcgggggcg
     9601 cccgaaactc ggcgagcttg cattttcgaa tgacctgacc ttcttctacg tggtgctcgg
     9661 attcttcgtc gcctcgctct tcgcgctgca tctcctggtg agctcgccct tcggcaagag
     9721 cctggtcggc atccgggagc gcgagctgcg catgcgcatc ctcgggtaca acacctggct
     9781 gcacaaatac atcgccttcg tgatcgcggg cgcgttcggc ggccttgccg gcgtgctgtg
     9841 ggcgcacctg aacggcatcg tgagcccgag cgacgtgatc ctcaccacgt cggtcgacgc
     9901 gctcctgatg gtggtgctcg gcggacccgg taccctggtc ggcggcctga tcggcgcgac
     9961 cgtcgtggtg ttcctgcgcg aatatctcgc caccctggtg cagtggtggc agtacgtgct
    10021 cggcgcggtc tacgtgctga cgattctcta tctgcccggc ggaataatgg ctctcttcaa
    10081 aggaggaaga cgatgaagcg cttcacattg atattggccg gagtatttgc ggcaagtcct
    10141 gcttatgcac aagaactgaa aatcggcttc ctcgcgccga tgacggggat cttcgcgcag
    10201 atcggcaagg acatggagaa cggcttccag atgtacctcg acgagcagaa gagcaatttc
    10261 gcgggcgcaa gagtgacctt catcgtcgag gacgaggaag gcaagccccc ggtcagcgtg
    10321 cgcaaggccg agaagctcgc gcgccaggac aacgtgcaca tgctggtcgg cggcctgctc
    10381 gcctccaccg gctacgcgat cgcgccggtc agcacccgcc tgaagaccgt gtatatcccc
    10441 tcgatcccgg ccgccgacga ccttacgcag cgcgacctca aggactatcc gtacctggtg
    10501 cgcaccggct ggaccagctc gcagccgcac cacccgctcg gaagatgggc ctgcgacaac
    10561 aagtacaagc gcgtggtggc gatcgccgcc gactacgcct tcggccacga ggtggtgggc
    10621 ggcttccaga aggtgttcga ggactgcggc gggcagatcg tgcagaagat ctggccgccg
    10681 ctcgggacca aggacttcgg gccctacatc ccgaccatca agcaggacgc cgacgccatc
    10741 ttctcgctga tggtcggccc gatggcgctg cagttcccga agcagctcca ggaatcgggc
    10801 gtcaagaagc cgatcatcgg cggcggcacg agctacgacg agttcgtgct tccgtccatg
    10861 ggcgacgagg tgctcggcca cgtctcggcg ctgcagtaca gcgccgcgat cgacacgccg
    10921 aagaaccgcg agttcgtgcg caagtaccgc gagaagtacg gcaaggtgcc ctcctactac
    10981 tccgagagca actacaccac cgcgcagatg atccacgagg tgatgaagca gaccaagggc
    11041 aagtggcccg ggccggagaa gttcgtcgcg atgatggccg gcctcaagat cgacgcggtg
    11101 cgcggcccgg tgcgcttcga cgagatgagg aacccggtgc agaacatcta catcaagaaa
    11161 gtcgagaaga agaagatgca cggctacgac aaggacgagc tctggaacag cgtgatccag
    11221 acctatccca acgtcgggca gttctggacc tacaagaagg aagagttcct gaagcagccg
    11281 gtgtacagcc gcgacttccc gccttgcaag tactgcaact aaaggggctg tcgaaaagct
    11341 tcggtggcct cgcggcggtc cgcgaggtca ccctggaggt tcatgccggc gagcgcaagg
    11401 ccatcatcgg cccgaacggc gccggcaaga cgacgctctt caacctgatt acgggggttt
    11461 tcccctgttc gtccggaagc attgtccttt ttgaaaaaga cgtgacgcgc tggccgagcc
    11521 accggcgtac cgcgctcggc atggcgcgca cgttccagat caccagcctg tttcccaagc
    11581 tcacggtgct cgacaacgtg ctgctcgcgc tcgccggggt gggctggacc aagctggtga
    11641 tgtggaaacc gctcgcttct tatgaaaagt tcaaaacccg ggcaagacaa ctgctggagg
    11701 cggcgggcta ctgggaccgg cgcgacgtcg agatccgcaa cctgtcgcac ggcgagcagc
    11761 gccagatcga ggtggtgctc gggctcgcga gcgacccgaa gctgctgctg ctcgacgagc
    11821 cggcggccgg gctctcctcg ggcgagtcgc gcgagatggc cgagttcctc aagcgcctcg
    11881 atccctcgct cgccatcctg ctcatcgagc acgacatgga cgtggtgttc gacgtggtga
    11941 gcgacatctc ggtattgcac ttcggcgccg tggtggagtc cggctcctgc acccagatac
    12001 agaaaagcga gcgcgtgcag cagatctacc taggcacggg atgattctcc aagtcgacga
    12061 catccacacc tactacgggg atgcctacgt cctgcaggga ctgtcgctct cgctcgagaa
    12121 gggccagatc ctcggcctgc tcggcagaaa cggcgtcggc aagaccacgc tggtcaactc
    12181 gatcgtcggc ttcaaccggc cgcgccgcgg ccgcatcgtg ttcaagggcg ccgacatcac
    12241 gcgcctctcc tccttcgaaa cgatcaggaa cggaatgggc ctggtgcccc agggccggcg
    12301 ggtgttcgcc tccctcacgg tggaagagaa tttgaaagta gcgcgaagcg cagaaggcgc
    12361 gtggaccctg ccccgcgtgt acgcgctgtt tccgcgcctg gaagagcgcc gccgccagcg
    12421 cgccaagacg ctctcgggcg gcgagcagca gatgctcgcc atcgcccgcg gcctcatgac
    12481 caatccggac tgcctgatca tggacgagcc ctccgagggc ctcgcgccga tcatcatcca
    12541 ggggctgtgg caggcaatcg cgaagctcaa ggcgcagggc ctgtcgatcc tgctcgtcga
    12601 gcagaacgcg gcgctcgcgc tgcagttagt tgactacgtg cacgtcatca gcaaaggccg
    12661 cgtcgtctac tcaggcgccc ctaaggcgct ctgggccgac gaggacgtca agtcccgcta
    12721 tctcggcatc tagaaaaaat cagacctcga tccgccctcg cagcccagcg aagcgcagga
    12781 agccttcgta ggcgcgtatt tcgcgcgcga ggaaatcgac cacgcgctcg aacgggtcga
    12841 agccgaacgc ctgcgcgacc gagggccgca ggttggagtt ggcgttgacg tcgtagaaca
    12901 ccaggcgccc gtcgcgcgtt tcgaggtact cgatgccggc gacgtcgagc ttgccggcgg
    12961 ccacgatgcg cttgccccag gcgactgcct gcgcgggcac gtccttgtag ggatagaact
    13021 ccgcgggcgg gacagtattt tccacttcac agctcgcggt ctcgctattg cacaccggcg
    13081 aggggcacag attgaagcgc ccatgcgaaa tcacgcgcat ggcatagagc agctcgccgc
    13141 cgaggaactc catgcgcacg atccctttcg ccgggtcggt gtcgaggtat tcctgcagca
    13201 gcagcaggtt gtccggcaac cagtggtccg gatgctggtt tagaaatgcc tccagttgtt
    13261 gaaaactctc cacgagcagg attcgggcgc cgctgccgcc ctgctcgggc ttgagcagca
    13321 ccgggaacgc gatctcgtgc gcccgcgcgc ggagcgcctc gaggtggttg aacgtccaca
    13381 cgcgcggcgc gggcacgccc aggttgcgca atagcgcggc ctgggcggtc ttgctgatct
    13441 ccagggcgaa cgcgtcggcg ccgttcagca cgcgcacgcc gcggatctgc agcgcccgca
    13501 tgtgcgcgag cgcatacggc accgcgcgga cgttgccgcg cacgtaggcc gacgggctcg
    13561 cctggttgaa atagagcggc gcgagcggct cgtcggcgtc ggcgaacgca gcccgcttga
    13621 ggtccacggc cgcgtagcgg atgccgcggc gctcgagcgc ggcgaagagc ggcttctgcc
    13681 actcgggatg ctcgtacagc actgccagat cgttgggata gctcatccgc gtattgtcta
    13741 ggctcgagca gccttggcga acgaacgaaa aagaatgacc ttatgcgatc ggaaactgcc
    13801 tgtgcctcgc gatccggcgg caacgaagtg atgcaagaag ttatcaggac catggcactg
    13861 cgtagcagtc cacgggcagc ttcacctccc tgaggtaaca tcgccgccgt gagagcctca
    13921 ctcgccatcg cgatcctcgc cctcgccggc tgctcgagca cgccgcagaa ggcgcccgtt
    13981 gccggatcgg gctcgacccg gccgacggcc gacatcgcgg gccaggccgc gggttatgcg
    14041 cagacgatga tcggcaagcc ctatcgctac ggcggcacga gccccaaggg tttcgactgc
    14101 agcggcctcg tgttctacag ctacaagcag gcgggcgtcg cactgccgca cagcaccgag
    14161 aaacagcgct ccgccgcacg cagcatcaag atggcggagc tgcgccgcgg cgacttgctg
    14221 ttcttcaacc aggaaggcaa gaagtacggc cacgtcgcga tctacgtcgg cgacggcagg
    14281 ttcgtccacg cgccctcgtc gggcaagtcg gtgcgcagcg accgcctcga caccccgtac
    14341 tggaagaagc acctctccga agcgcgccgg atttagtctt cctggatcac cgccgggccc
    14401 tcggtgctcg ccgcgcgcgc gggcgacgtc cttcgcggga atgtaggcgt acatgggcga
    14461 tttctgtagc atggcgctct cctcggttga ccgaaaatgg aatctcatgc aaaaaacaag
    14521 cgttcgcttt ctcaccgcgc tgctcggcct cttggttagc gcccaggtgg cggcggcgac
    14581 cttcgtctac gtctcgaacg cggaggacgg cgatatcggc ctgtatacgc tgcggcccga
    14641 cggctcgttg cagcccggcc agcgcttcaa ggccgggccg atggtgatgc cgatgtcggt
    14701 cagcccggac aagcgcttcc tcgtcgccgc ggtgcgctcg aagccgttct cggcgcacac
    14761 ctacagcatc gaccgcggca ccggcgcgct caagccgatc ggcagcgggc cgctcgccga
    14821 gagcttcccg tatatcgcct tcgaccgcac cgggcgccac ctcctcggcg cgtcctatgg
    14881 cgcgaacctc gtcagcgtca atcccgtgaa cccggatggc acgatcggcg cgccggcgca
    14941 ggtgattccc accgcgcgca acgcgcacgc gatcatcacc gacaggacga accgcttcgt
    15001 attcgtgccg cacctcggca ccgaccaggt gttccagttc cagttcgacg agaagagcgg
    15061 ccggctcgcg gcaaacacgc cgccggtgct gcagctcaag gcgggcagcg gcccgcgcca
    15121 cctgatcacc tcgcccgaca accgcttcgt ctacctgctg aacgagctca ccgccaccgt
    15181 cagcacgctc tctctcgaga acgggttgct gaaggaagtc tcttcggcct cggcgctgcc
    15241 gccggagagc aagctcggcc ccggcatgcc gcgcccgcag cagggccgcg acgtcagcaa
    15301 cgacatctgg gcttccgacc tccacctcac gcccgacggc cgcttcctgt acgccgccga
    15361 gcgcaccggc agcacgatca acggcttctc ggtggacagc gcgagcggaa agctgaccta
    15421 cctggggtcg acgccgaccg agaagcagcc gcgcggcttc cgcatcgatc cgaccgggca
    15481 cttcatggtg gtgtccggcg agaagtcgga aaccatctcg agctaccgga tcgaatcgaa
    15541 cgggctgctg gtgcccgtcg gcagggtcgc gaccggcaag ggctcgaact gggtcgagat
    15601 cgtcagcttc gactgaaaaa aggaatgcta acctagcatt tcgccctaac ctcccggaga
    15661 cctgcatgcc cgcttcacgc cttttcgccg ctttcctgct tgccgcgttc agcgcccttg
    15721 ccttcgccca ggcgcagaaa gcccctgccg cctacgagcc gacggtcggc caggaaggaa
    15781 aagatgtcgt ctgggtcccg accccgcagg ccgtggtcga caagatgctc gacatggcca
    15841 aggtgacctc gaaggacttc gtcatggacc tcggctcggg cgacggccgc accgtgatca
    15901 ccgcggcaaa gcgcggcgcg cgcgccgtgg gcgtcgagta caaccccgac atggtcgagc
    15961 tttccaagca caacgccgag aaggaaggcg tggccggcaa cgccaccttc atgaaggcgg
    16021 acctgttcga gaccgacttc tcgcaggcga ccgtaatcac gatgttcctg ctgcccgaca
    16081 tcaacatcaa gctgcgcccg aaaatcctct cgctcaagcc cggcacgcgc atcgtgtcga
    16141 acagcttcac catgggcgac tggaaggcgg acgaaaccgc cgagctgaac tcttctcagg
    16201 gctgcaacaa ctcctggtgc accgcgctcc tgtggatcgt cccggcgaag gtcggcggca
    16261 cccacaaggt cccgcagggc gagctcatgc tcaagcagga gttccagatg ctctccggca
    16321 cgctgcgcgc cgacggcaag acctacgccg tgacgggcaa ggtcaacggc gaggaagtcg
    16381 tcttcaaagc gggcggccgc gaataccacg ccagaatgaa cggcaagcag ctcgagcttc
    16441 acaaatgaga cgcctgtgcg ggctgctgct ggcggcagcg ctcgcattgc ccggcgccgc
    16501 tgccgcggcg tatcccgaca agccgatcag cctggtcgtc gcctattcgc cgggcggcgg
    16561 caccgacctg atcgcgcgcc agatcgctcc gttcctcgag aaatacctcg gcggcggcgc
    16621 gcgcatcatc atcgtgcacc ggccgggcgc cggcggcgag atcggcttcg cggcgatcgc
    16681 caacgccgcg cccgacggct acaccatcgg cttcgtcaac accccgccgc tcatcaccgt
    16741 gccgatcgag cgcaccgcgc agttcggcgg cccgcagcgc ttcgagctgc tcggcaacgt
    16801 gatcgacgac ccgtgcaatt tcaccgtgca tgcggactcc gagattcgcg acctgaaagg
    16861 gctggcggcg tatgcaaaag caaatcccgg caaggtgacg gtgggctcga gcggcatcgg
    16921 ctccgacgac cacctgctga tgctgatgtt cgagcgcgcg gcgggcgtga agatgaccca
    16981 catcccgttc aagggtgcgg ccgacgtgcg caccgcggtg ctggcgaagc agatcatggt
    17041 cggcgcgatc aacatcggcg aggcggttca gtcgatcaag ggcggcgcgc cgctgcgcaa
    17101 catcggccag ttcagcccgg cgcgcactac ggtcgcgccg gagctgccga ccgcgcgcga
    17161 gcagggctac gacatcgagc tctcgtcgct gcgcggcctc gccgcgccga ggggcctgcc
    17221 tgccgacgtg cgcgagcgcc tggtgaaagc ggtggccgac gcggccaacg acccggagtt
    17281 ccaggcgaag gcgaccagcg tgttcgcgcc gctgcgcttc ctcaccccgg cgcagttcga
    17341 cgcggtgatc agggaagccg aggtgggatt caagcagctc tggaaagagc tgccctgggg
    17401 cgacaaatag aattcggggt cagggtgcga attcgttttt gcgtacgtga aaacgaattc
    17461 gcaccctgac cccgaattcc gctaggcagg ctcgatcgtg ctgtcctcgg gcatcgccgc
    17521 gagctttttc ggcagcggct cggtcgattt gccgaacttg cggcgcttgc gctcgttcca
    17581 ctgcgcgagc atgcgcggcg cttccgcggc gagcttgtcc caggagtcgg gctgctcggt
    17641 gcgcaccgtg atctcgaggc ggtggccgtc gggatccatg aagtagatcg actggcagaa
    17701 gtcgtggtcc ttgggtccga gcacctgcac gccgtggctt tcgaggcgcg ccttggcggc
    17761 gagcagcttc tccatgctct cggtctcgaa ggcgatgtgc ttcacccacc acggcgtgat
    17821 ctcgggcgcc tcctcgggcg ccgaggcgac gtcgaagaac gcgaggtagc tgccgtcgcc
    17881 gagctcgaag aagatgtgga tcgagtccgc cggcgcgccg accaccttgc gcggcggccg
    17941 gcccggctcg ttcagcggcc aggaagggtt gctgcggctc tcgggcggca gcaggccggc
    18001 gcaatatttc aggccgacca ccttggtata gaagtcgacg gtgcgctgcg cgtcgcggca
    18061 gcggtaggcg atgtggtgca ggcgctcaaa catgaccggc gttcccttcc gttcgataag
    18121 gcattctgaa tagtaggcgc ggagcttgct tgcttcaaga agtaccaaga ggtaccccat
    18181 gagccttgtt tccgaaagac aaaacggcgc aggcgcagcc gctcgcctgg agccgttcgc
    18241 cggccactgg gtggtcgaag gcgaagtgca gaagagcgcc tacggcccgg cgtcgcgctg
    18301 gacttcggac gagcgctgtg aatggctgcc cggccagcgc ttcctcgtca accgctggga
    18361 tgcgcgcgtc ggcgagcgcg acttcagggg catggccgtg ttcggatacg acccgcaacg
    18421 cggctacttc gcgcgcttcc acgaccatgc cggcaacgcg ccggactacc gaatgaacat
    18481 cgatggcccg acctggaccc tggccgggga cgcgcagcgc gcggtgtatg aattcggcac
    18541 gggaaccatc gccatccgct gggaatggcg cgacgacaat ggctggcact cgctgcgcac
    18601 gttgcgcgcg cgccgggggg tgagcgcctc ggaaatggtg catgaatact tcgccgcctt
    18661 ccaggcgcag gaccgcgcca cggccgagcg cctgctcgcc caggacttcg tcttcagcag
    18721 cccgcgcgac gaccgcatcg accgcgccgc atacttcgag cgctgctggc cgaacgcgag
    18781 ccggctgcgc gctttctgga tggagcgcct gttcgagcac gacggcgagg tgttcgtgcg
    18841 ctacagcgcc gagcgcctgg ccgacggcgt gcgcttccgc aacaccgaga tcatccgcgt
    18901 ggcgcgcggg cagatccgcg agatcgacgt ctacttcggc cgcgacctcc ggtgaattcg
    18961 gggacggagc cctaactccg gaagctgcgc aagccgtgga gttagggctc cgtccccgaa
    19021 ttcatttccg gagaaaagcc ctcaccaccg gcaccgccac cgccagtagc gccagcaccg
    19081 tcagcacgcc cgagatcggg cgcatgaaga ggatgccgag gccggcatcc gagatgatga
    19141 gcgactggcg caacgcgcgc tcggcgagcg gcccgagcac cagcgcgagc accagcgcgg
    19201 cgggcgagta gccgagcttc ttcatgaagt agccgatgac gccgaacacc agcatctggc
    19261 cgacgtccca caggttgttc ttgagcgagt acgcgccggt gacgcagaag acaacgatca
    19321 gcggcatcag gatcgagtag ggaatgcgca ccgcgcggac gaagagcggg atgcagagcg
    19381 tgctgaggat caagagcagg atgttggcga tgtactgcga ggcgatcagg ccccagacga
    19441 actgcgggtt cttctcgaac agcatcggcc cggggcgcag gccccacatc atcaggccgc
    19501 cgagcatgat cgcggtggtc gccgagcccg ggatgccgag cgtgagcat
//
LOCUS       DATBSZ010000077         5397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2264902, whole genome shotgun sequence.
ACCESSION   DATBSZ010000077 DATBSZ010000000
VERSION     DATBSZ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2264902"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2801
                     /locus_tag="VIG70_05195"
     CDS             <1..2801
                     /locus_tag="VIG70_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8249983.1"
                     /translation="LGPIEEALASAELPDAMRPQLDVAHRNSMRLLKLVNSLLDFARI
                     EAGRVTAAFEPVDLATLTEDLSSNFRAAMDRAGLRFDVSCPPLDAPVHVDREMWEKIV
                     LNLLSNAFKFTFEGEISVSLQHRGDAFELAVRDTGTGIPAAELPHVFERFRRVAGAKS
                     RSHEGTGIGLALVQELAKLHGGAIAAQSAEGEGSTFSVSIPAGKDHLPSERIGAGSAP
                     SPTGVNAEAFVEETLHWLPGGGTLSSKEDLSTHGPRARIVWADDNADMREYVRKLLAT
                     RYEVEAVPDGEAALAAVRARLPDLVLADVMMPKLDGLGLLRALRADPQTHTIPAIVLS
                     ARAGEEGRIEGLDAGADDYIVKPFGARELLARVDSQLKMSRLRREASSKIAESEQGLQ
                     AELADAKLLQGIAIELVHEDNVNLLYDKIMDAAVAVMRSDFASLQMLYPERGSGGELR
                     LLAFRGFNPQAAKFWEWVRADSECTCGAALRAGQRVIAADVEKCDFMAGTEDLATYLK
                     TGIRAVQSTPLLSRTGKLVGMISTHWREPHRPTERDLRVLDILARQAADLIERSAGEQ
                     ALRDSREQLLEADQRKDEFMAMLAHELRNPLSAIALGADLLKRARLDDPKAQFAVPAI
                     ERQAKQLQRLADDMLDMARATYGKLTLKTERIHLLEMAKAVAALHARTGKSPELEVAG
                     EPAWVDGDAVRVQQMIGNLIENAVKYGGTKIKVRVRREEAWSRLSVQDDGQGIAPELL
                     PDLFKPFVQGEQSLDRAQGGLGLGLALVDRLAALHGGSVEAQSPGVGQGSTFTLSLPA
                     AAPAAAKDKAGPISAALKRRVLVVEDEKDVREMLRVLLESEGHEVSIADSGTDGLAKF
                     GSFRPDVALVDIGLPGMDGYELARQARSLPGGSRFKLVAITGYGQEKDRRLALEAGFD
                     LHVTKPVVYDQLARALET"
     gene            complement(2806..3093)
                     /locus_tag="VIG70_05200"
     CDS             complement(2806..3093)
                     /locus_tag="VIG70_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249984.1"
                     /translation="MKTALQAQIEDLFSRHPALCGFSVRGVDELPDHCPRGEEHDGEL
                     FVDDIGVLPVLGREQLGEILQEIVAVLAEFIADEPGAGEALRGRTFARVLH"
     gene            3314..3436
                     /locus_tag="VIG70_05205"
     CDS             3314..3436
                     /locus_tag="VIG70_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249985.1"
                     /translation="MERLQQWRRKQEGKLGYILLWALGVPIPVLFVIFLLRGCN"
     gene            3437..3793
                     /locus_tag="VIG70_05210"
     CDS             3437..3793
                     /locus_tag="VIG70_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249986.1"
                     /translation="MDPLIYKLVAAAFAAAFFASSSHAAPRAQSAEECGIAADMALVA
                     QSLAREEIQPAKASAIMARIYDVAESERGQELMRSILGAAYTRNVSQGTPFAEELFTA
                     CMSSGGNMDKILGRSL"
     gene            complement(3809..4108)
                     /locus_tag="VIG70_05215"
     CDS             complement(3809..4108)
                     /locus_tag="VIG70_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249987.1"
                     /translation="MIHLASQFKADIYLAGRDPLHAWALAQRRRIDLGGSGAWIAPPE
                     YVIVRKLEYLREGGSDKHLRDIRFILAATSIDRTFVETEVARLGVGAQWQRCQQR"
     gene            complement(4210..4428)
                     /locus_tag="VIG70_05220"
     CDS             complement(4210..4428)
                     /locus_tag="VIG70_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249988.1"
                     /translation="MKDILPADPEALHPVQVEGFRRMSPARKLQMVADLYAAGIRLRA
                     AGLRLAHPDWTEDELQRQARRSLLHAGT"
     gene            complement(4513..4950)
                     /locus_tag="VIG70_05225"
     CDS             complement(4513..4950)
                     /locus_tag="VIG70_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8249989.1"
                     /translation="MTLPDTRWTQEFHRRRGEGPLRVIVADDDADTVATLSAILQDAG
                     YLVHAVSNGKDVLPAARFVRPDAMILDISVPGMSGYAVAEVIRNSFTEARRPLLIAIS
                     GWWKEPSDRRVAQHVGFDHHLLKPCDPQDVLALLEPLRRPDPA"
     gene            complement(5043..>5397)
                     /locus_tag="VIG70_05230"
     CDS             complement(5043..>5397)
                     /locus_tag="VIG70_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8249990.1"
                     /translation="LRLEGHEVKAVHDGLQALSSLKVFDPHVVVLDIGLPGLDGYAVA
                     RQLRERGDTSHVLLIALTGYGQKEDRQRAADAGFDYHYVKPADPREIQAAIERGRNTG
                     GEPGLEAHRIGTGSN"
BASE COUNT          880 a         1836 c         1873 g          808 t
ORIGIN      
        1 tgctgggacc catcgaagaa gcgctcgctt cggcggagtt gcccgacgcg atgcgccctc
       61 agctcgacgt tgcgcaccgc aactcgatgc gattgctcaa gcttgtcaac tcactgctcg
      121 actttgcgcg catcgaggcc ggccgcgtca cggccgcatt cgaaccggtg gatctcgcca
      181 cgctcaccga ggacctgtcc agcaattttc gcgccgccat ggaccgcgcc gggctcaggt
      241 tcgatgtgag ttgcccacca ctcgatgcgc cggtgcacgt cgatcgcgag atgtgggaaa
      301 agatcgtcct caacctgctg tcgaacgcgt tcaagttcac gttcgagggc gaaatatcgg
      361 tttccctgca gcaccggggg gacgctttcg agctcgcggt gcgcgacacc ggcaccggga
      421 tccccgccgc ggagctgccg catgttttcg agcgcttccg ccgcgtcgcc ggcgccaaga
      481 gccgcagcca cgagggcacc ggcatcggcc tcgccctggt tcaagagctt gccaagctcc
      541 acggcggcgc gatcgcggcg caaagcgccg agggcgaggg cagcacgttc agcgtctcca
      601 tccccgccgg gaaggatcat cttccgtccg agcgaatagg cgcggggagc gcaccctccc
      661 cgaccggcgt taacgccgaa gccttcgtgg aagagacgct ccactggctt ccaggcggag
      721 ggacgctatc ttcaaaagaa gacctctcca cgcacggccc gcgcgcgcgc attgtctggg
      781 ccgacgacaa cgccgacatg cgcgagtacg tgcgcaagct cctcgccacg agatacgaag
      841 tcgaagcggt gcccgacggc gaggcggcgc ttgcggcggt gcgcgcgcgc ctccccgacc
      901 tggtgctcgc cgatgtcatg atgccgaagc tcgacgggct gggcctgctg cgcgcgctgc
      961 gcgccgatcc gcaaacgcac acgatcccgg cgatcgtgct ctcggcgcgc gcgggcgagg
     1021 aaggacgcat cgagggcctc gacgcgggcg cggacgacta catcgtcaaa cctttcggcg
     1081 cgcgcgagct gctggcgcgc gtcgattcgc agctcaagat gtcgcgcctg cggcgcgagg
     1141 cgagctcgaa gatcgccgag tccgagcaag ggctgcaggc ggagctggcc gacgccaagc
     1201 tgctccaggg catcgccatc gagctggtcc acgaggacaa cgtcaatctg ctctacgaca
     1261 agatcatgga cgccgcggtc gcggtgatgc gctcggattt cgccagcctg cagatgctgt
     1321 acccggagcg cggcagcggc ggcgagctcc ggctgctcgc gttccgtgga ttcaatccgc
     1381 aggccgccaa gttctgggag tgggtgcgcg ccgattcgga atgcacctgc ggcgccgccc
     1441 tgcgcgccgg ccagcgggtc atcgcggcgg atgtcgagaa atgcgacttc atggccggca
     1501 cggaggacct ggcgacgtac ctgaagaccg gcatccgcgc ggtccagagc acgccgctct
     1561 tgtcgcgcac cgggaagctc gtcggcatga tctccacgca ctggcgcgag ccgcaccggc
     1621 ccaccgagcg cgatctgcgc gtgctggata tcctcgctcg ccaggccgcc gacctgatcg
     1681 agcgcagcgc gggagaacag gccctgcgcg acagccgcga gcagctcctg gaggccgacc
     1741 agcgcaagga cgagttcatg gcgatgctcg cgcacgagct gcgcaacccg ctgtcggcga
     1801 tcgcgctcgg cgccgacctg ctgaagcgcg ccaggctcga cgaccccaag gcgcagttcg
     1861 ccgtgcccgc catcgagcgg caggcgaagc agctgcagcg cctcgccgac gacatgctgg
     1921 acatggcgcg ggccacctac ggcaagctca cgctcaagac cgagcgcatc catctgctgg
     1981 agatggcgaa ggcggtcgcc gcgctgcacg caaggaccgg gaagagcccg gagctcgagg
     2041 tcgccggcga gcccgcgtgg gtcgacggcg acgccgtgcg cgtgcagcag atgatcggca
     2101 acctgatcga gaacgcagtc aaatacggcg gcacgaagat caaggtgcgc gtgcgtcgcg
     2161 aggaagcctg gagccgccta tcggtgcagg acgacgggca gggtattgcg cccgagctgc
     2221 tgccggacct gttcaagccg ttcgtgcagg gcgagcaatc cctcgatcgc gcgcagggcg
     2281 ggctgggcct cgggcttgcg ctcgtggacc ggctcgctgc cctccacggc ggcagcgtcg
     2341 aggcgcaaag ccccggggtc ggccagggca gcacctttac cctctcgctg cccgccgcag
     2401 ccccggcggc agcaaaggac aaggcggggc cgatctccgc ggcgctcaag cggcgggtcc
     2461 tggtggtcga ggacgaaaag gacgtgcgcg agatgctgcg cgtccttctc gagagcgaag
     2521 gccacgaggt gtcgatcgcg gacagcggca ccgatggcct cgccaagttc ggctcgttcc
     2581 ggccggatgt cgcgctggtg gatatcgggc tgccggggat ggacggctac gagctggcgc
     2641 gacaggcgcg ctcgcttcca gggggcagcc gcttcaagct cgtggccatt accggctatg
     2701 ggcaggagaa ggaccggcga ctcgccctcg aggccggctt cgacctccac gtcaccaagc
     2761 cggtcgtcta cgaccagctc gcgcgggctc tcgaaaccta gcgaatcagt gcaggacgcg
     2821 ggcgaaggtg cggccacgca gcgcctcgcc ggctccaggc tcgtccgcga tgaactcggc
     2881 aagcacggcc acgatctcct gcaggatctc gccgagctgc tcgcggccga gcaccggcaa
     2941 gacgccgata tcgtcgacga aaagctcgcc gtcgtgttcc tcgcctcgcg ggcaatggtc
     3001 gggcagctcg tcgaccccgc ggaccgagaa gccgcagagc gcgggatggc gcgagaacag
     3061 gtcttcgatc tgcgcttgca gtgcggtttt catgggccca tggtggacgc gcaggcagcg
     3121 cctggatgcc gccgggcgcg gcgccgggcc gtcgaactct cctgaaattt cccgcgccgt
     3181 tcgatgacgg gcgtttccct tcacgcgaaa aacccttgtc ggacttggcc ttgcaccgtt
     3241 cgcagcgccg cgcgcgaccg caccggcatc acgcttgctg cgtacgctga tcgatcatcg
     3301 acccggaggc aatatggaaa ggctgcagca gtggcgtcgg aaacaggaag gcaagctcgg
     3361 ctatatcctc ttgtgggcgc tcggcgtgcc cattcccgtg ctgttcgtga ttttccttct
     3421 gcgcggctgc aactagatgg accccctcat ctacaagctg gtcgcggcgg ccttcgcggc
     3481 cgccttcttc gcatcgtcga gccatgccgc gccgcgcgcg cagagcgccg aggaatgcgg
     3541 catcgccgcc gacatggcgc tcgtcgcgca atcgctggcg cgcgaggaaa tccagcccgc
     3601 caaggcgagc gcgatcatgg cgcgcatcta cgacgtggcg gagtccgagc gcggccagga
     3661 gctgatgcgc tcgatcctcg gcgcggccta caccaggaac gtgtcgcagg gaacgccgtt
     3721 cgccgaagaa ttattcaccg cctgcatgtc gagcggcggc aacatggaca agatcctcgg
     3781 ccgcagcctg tgaccgagtc tccctaggct aacgctgctg gcagcgctgc cactgcgcgc
     3841 cgacgccgag gcgcgcgacc tcggtttcca cgaacgtgcg gtcgatcgaa gtcgccgcga
     3901 gaatgaagcg gatatcgcgc aggtgcttgt cggaaccgcc ctcgcgcagg tattcgagct
     3961 ttcgcacgat gacgtattcg ggcggggcga tccaggcgcc gctgccgccg agatcgatcc
     4021 ggcggcgttg ggcaagcgcc caggcgtgca gcgggtcgcg cccggcgagg tagatgtccg
     4081 ccttgaactg gctcgcgagg tgaatcacgt tgaacgcacc gcgcgcgctg cgcgcgagct
     4141 ccagacgcag cgtctcgtcc ggcggcaggt agtacgagga ttgcggaaac gcggcgcggc
     4201 gcgagcggct caggtgccgg catgaagcag cgaccggcgc gcctggcgct gcagctcgtc
     4261 ctccgtccag tccgggtgcg cgaggcgcaa gcccgccgcc ctgagtcgta tcccggccgc
     4321 atacaggtcg gcaaccattt gcagtttccg ggccgggctc atccgccgga agccctcgac
     4381 ctggaccggg tggagcgctt cgggatcggc cggaaggatg tccttcatgg cgcggacatc
     4441 ataccccccg acccgctcga cggaccgcac gccccgccgg ccggtgcccc cgcaaaagcc
     4501 gccgcccggc gactaagccg ggtcggggcg gcgcaacggt tccaggagag cgagcacgtc
     4561 ctgcggatcg cagggcttga ggagatggtg gtcgaagccg acgtgctgcg ccaccctccg
     4621 gtcggacggc tctttccacc agccggaaat tgcgatcagc agcggacggc gcgcctcggt
     4681 gaagctgttg cggatcactt cggcgaccgc gtagccgctc attcccggca cggaaatatc
     4741 caggatcatc gcgtccgggc gcacgaagcg agcggccggc aggacgtcct tgccgttgga
     4801 cacggcatgc accaggtacc cggcgtcctg cagaatcgcc gaaagggtgg cgacggtgtc
     4861 cgcatcgtcg tcggccacga tgacccgcag cgggccctcg ccgcggcggc gatggaattc
     4921 ctgcgtccag cgagtatccg gcagggtcac gagcgcttgg ccggagtgtg atccccggca
     4981 ggcgacattc ttcctgcgct gaatagtccg aacggtccga ccgggtgcgc cgatcgtcgg
     5041 gcctagttcg agccggttcc gatgcggtgg gcctcgagcc ccggctcgcc gccggtgttg
     5101 cggccgcgct cgatcgcggc ctggatctcg cgcggatcgg cgggcttgac gtagtggtag
     5161 tcgaagccgg cgtcggcggc gcgctggcgg tcttccttct ggccgtagcc ggtgagcgcg
     5221 atcagcagca cgtggctggt gtcgccgcgc tcgcggagct ggcgcgcgac ggcgtagccg
     5281 tcgaggccgg gaaggccgat gtcgagcacc accacgtgcg ggtcgaacac cttgagcgag
     5341 gaaagcgcct gcaggccatc gtgcaccgcc ttcacctcgt ggccttctag gcgcagg
//
LOCUS       DATBSZ010000078         9114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_79480, whole genome shotgun sequence.
ACCESSION   DATBSZ010000078 DATBSZ010000000
VERSION     DATBSZ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9114
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79480"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VIG70_05235"
     CDS             complement(<1..671)
                     /locus_tag="VIG70_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8249991.1"
                     /translation="MSRKSVLISYFFGDDMIPLGVSCANAFRDLGYDVLRFNSQVESR
                     WQPALLKPVNRLARGLGYRGRELGARLPVARINFKKRMLERAARKFRPRWVFVIRAHE
                     FVDAELVARLKRDCGVEKVFAWRVDGPLDTPDLLEDAAVYDRYFCSHRHGYDPRRDRI
                     DFLPVYGMDFSLYRNLHVGTYEHELVLVGGHNARREQLVAGLLELPLEIYGKWGRTRS
                     KLSLTG"
     gene            676..1284
                     /locus_tag="VIG70_05240"
     CDS             676..1284
                     /locus_tag="VIG70_05240"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8249992.1"
                     /translation="MRLRNPQIARDLEQGQPLRLDIGSGPRPRPGFYALDQIELDGID
                     IVADLNQPLDLLPDACAEHIFASHALEHVEQLLPLIAEIHRIARPGALVEIIVPHFSN
                     PYFYSDPTHVRPFGLYTMNYFVDTEKQPEVWRVQTFYSKARFEMESVKIAFYRFNLWD
                     RLFVPFLRYFVNRSARTQNFYELRLSWLFPAAEIRYRMRACK"
     gene            1289..2347
                     /locus_tag="VIG70_05245"
     CDS             1289..2347
                     /locus_tag="VIG70_05245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8249993.1"
                     /translation="MLQRVLIAYTSKPPIINYLTAAFARRGIEVRAVFADENTWFDRL
                     VIHRLNKLAHNFRVIPKSKNFFENRPASHMNFRSARLRDAISAYDPDLVFHIRGLGFR
                     PLAFEGARRKFAWWVESDERVDEALAEVPWFDRYFFINSANVEAAQRAGYGHASYLPH
                     AVDASVFRPLPGVVKDIDFAFVGLWSERRQKFIEAALEVSGNGALYGPKWYRKTFPDR
                     RFRRIFRGRYIDGAPLVRLYNRAKVVLNITTWGANGGANRSGMTMRTFEAPATGSLLL
                     TDHSAELSLAVTPGTHVDTFSDLDDFKKKLHYYLDHAPERQRIAVHGMQHVRVHHTYD
                     AMVDSILAAYEDLPTGER"
     gene            2350..3555
                     /locus_tag="VIG70_05250"
     CDS             2350..3555
                     /locus_tag="VIG70_05250"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY8249994.1"
                     /translation="MHPLSLSAWAAAFFVSATLFAHTVAFRLLMLACCAGLALVAIAR
                     ERGAIRALPPIWPALALWTAWALLSLSWSMDPERTAKELRNEVGYTALAFWACYVAGQ
                     AHHAVRVVAPVVGAAAVLLCGLAFYYFPHGNQWNPPPWPGGPGNLSSTLLTLMPCALL
                     GAWYGQRARDRLVSLASLALVVLLFAAAYTTLNRTIWVGFAAQFLLIGALLASRKVRP
                     MPLRAKVVGTALATLIVASAALATFQIQMARQQAIPAAALDRDSRISLWPQVLEHVKE
                     RPLTGYGFGRGMLRSSLLKEFDDTFLWHAHNLFLDTVVQVGLPGLVLLLFLLGATVRE
                     GWRLARASDRFAAACGFALLCVVAGMVMRNMTDTLWVRQNALLYWGVLGLLLAWGHTA
                     RTSASRRST"
     gene            complement(3532..5433)
                     /locus_tag="VIG70_05255"
     CDS             complement(3532..5433)
                     /locus_tag="VIG70_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEY8249995.1"
                     /translation="MNSRSLFAFAHDVVAAALAWLAAFWLRFNFEIPESYQELMLERL
                     PWAAAIYAVLFWRLGLYRGLWRYASLPDLQRIVIAVGVGALAVPAVLIVAGAGASVPR
                     TVYVLTPMLLILVMGGSRLAYRAWREGRLLPIVSKPTATPVLVLGAGSFGNTLLKELA
                     ASAQWRVVGLLDDDPRKQGAEILGVKVRGPIDRVGEIANAHRVTQAIIAMPGASHTSR
                     RRALDLCAQAGLSVMTVPAISDIVSGRVSVSALRKIELDDLLGRDPVQLDDAGLHAFI
                     DGKAVLVTGAGGSIGAELCRQIVRFAPARLVLLDNSEFALYSIEQEFRDRHPAVRVAA
                     VLGDAKDERRVAEVFSRYRPQVVFHAAAYKHVPLMEDDNAFEGVANNVVSTVCVARSA
                     QRAGAEKFVLVSTDKAVNPTSVMGASKRLAEMVCQALQRGSGTQFTIVRFGNVIGSNG
                     SVVPRFREQISRGGPVTVTHPDIQRYFMSIPEAAQLVLQAGMMGKGGEIYVLDMGEPV
                     RILDLARQMIRLSGLAEGEIRIEYTGLRPGEKLYEEPLADAEKTLPTPHPKLRVAQAR
                     APENGRLLGEVVEWLTSPGEKSVDRVRDKLRIWIPEYVVELRPEAKPRAQAESFRQAA
                     ATLGRPPRR"
     gene            complement(5430..6404)
                     /locus_tag="VIG70_05260"
     CDS             complement(5430..6404)
                     /locus_tag="VIG70_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEY8249996.1"
                     /translation="MIGGLAIASFVVAWAAGRLLASRLRSVALDQPNARSLHERPVPR
                     TGGIAILLGAALALGLGAGELWPLMAMALALAAVSFLDDLRGLPTVARLLAHFAACGA
                     FAWYVLSPMHPVELAVLIVAGVWITNLYNFMDGADGLAGGMAAIGFGAYSAAAGLAGH
                     LPLASMCAALAAASAGFLTQNFFPARLFLGDVGSIPLGFLVYALGILGWRDDIWPLWF
                     PVLVFAPFIGDATLTLLKRLLRMEAVWRAHRDHYYQRMVRMGVGHRGTALAAYAVMLA
                     CAIAALLARQQPPAIQASVFLGMLLLLGMLAAWVDVRWRRSAHDRETA"
     gene            complement(6415..7257)
                     /locus_tag="VIG70_05265"
     CDS             complement(6415..7257)
                     /locus_tag="VIG70_05265"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8249997.1"
                     /translation="MIVAVTGARGFVGATLCPALAARAHRPVALPLHGVAEVRLPPQA
                     DTVVHLAALAHGRGASPEELHRVNVDLADKVGRAAAAAGARMIFVSTVKVHGEQSDVP
                     LDEGCPVAPRDPYAESKARAEERLRRIAGLRLTVLRPPLMYGPGVKANFRALMSALAR
                     GVPLPLAGVANRRSILYAGNLADAVLCCIAREASIERTYLLSDGPVLSSGELCRRLGN
                     ALGRPARLFRLPTRLLEALPGLRALTRSLEVDDTAIRRELDWRPAFSQQEGLAATAAW
                     FGRG"
     gene            complement(7254..8381)
                     /gene="wecB"
                     /locus_tag="VIG70_05270"
     CDS             complement(7254..8381)
                     /gene="wecB"
                     /locus_tag="VIG70_05270"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEY8249998.1"
                     /translation="MKVATIVGTRPELIKLSRVLAELDRAAEHVLVHTGQNYDYELNE
                     IFFKDLEIRRPDHFLEAAAPSVGETIGNVIARSYALLQKLAPDAVLLLGDTNSCLAAI
                     SAKRLKIPLFHMEAGNRCFDERVPEEVNRRLVDHVSDINLVYTEHARRHLLAEGLRPD
                     TVIKTGSPMREVLGHYAARIEASDVLQRLKLQAGRYFVVSSHREENIDAEASFRALVG
                     ALQAVWQRHRLPTVFSTHPRTQKRLEAAGTDMAGADVRFLKPMGFFDYVRLQSSAYCV
                     LSDSGTLTEECSILGFPAVTLREAHERPEGMDVGTLVMSGLGAANVLEAIALVRRHHD
                     EGRRSAAVPDYKAADVSAKVVRIILSYTDYVNRRVWMKPPR"
     gene            complement(8386..>9114)
                     /locus_tag="VIG70_05275"
     CDS             complement(8386..>9114)
                     /locus_tag="VIG70_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEY8249999.1"
                     /translation="PEQAVLTNCIGARNIVQAIEQGRLPVATVVGISTDKACKPVNVM
                     GMTKALQERIFVAANILNARTRFICVRYGNVLASRGSVIPLFHEQIRAGGPVTITDPR
                     MTRFLLSLEQAVDTVLTALKEARRGETYVPRASSATILNLAKALIGERKVPLKTIDVR
                     PGEKMHEIMVSEEEIHHCARRGEYLVIRPMLPELRRDDANEPNALDKEFSSEDTVIDL
                     AGTAALLKKHRLMIEDAPAGELLR"
BASE COUNT         1474 a         3161 c         3028 g         1451 t
ORIGIN      
        1 ccggtcaggg aaagcttcga gcgggtgcgc ccccatttgc cgtagatctc cagcggcagc
       61 tcgagcagcc cggcgacgag ctgctcgcgc cgcgcgttgt ggccgcccac cagcaccagc
      121 tcgtgctcgt acgtcccgac atgaagattg cggtagagcg agaagtccat cccgtagacg
      181 gggaggaagt cgattctatc cctccgcgga tcgtagccgt gccggtggct gcagaagtag
      241 cggtcgtaga ccgccgcgtc ctcgagcagg tcgggcgtgt cgagcgggcc gtcgacgcgc
      301 caggcgaaca ccttctccac cccgcagtcg cgtttgagcc tcgccaccag ctcggcgtcg
      361 acgaactcgt gcgcgcgaat cacgaacacc cagcgcggcc tgaacttgcg cgccgcgcgc
      421 tcgagcatgc gcttcttgaa gttgatccgc gccacgggca gccgtgcgcc gagctcgcgg
      481 ccgcggtacc ccagcccccg cgccagccgg ttcaccggct tgagcagcgc cggctgccag
      541 cggctctcga cttgactgtt gaaacggaga acgtcataac cgaggtcgcg gaaggcattg
      601 gcgcacgaca cgccgagcgg aatcatgtcg tcgccgaaga aatagctgat cagtacgctt
      661 tttctggaca tcaaggtgag acttcgcaac ccgcagatcg cacgcgacct cgaacagggc
      721 cagcccctgc gtctcgacat cggcagcggc ccgcgcccac gccccggatt ctatgcgctc
      781 gaccagatcg agctcgacgg catcgacatc gtcgccgacc tgaaccagcc gctcgacctc
      841 ctgcccgacg cctgcgccga gcacatcttc gcctcgcacg cgctcgagca cgtggagcag
      901 ctcctgccgc tcatcgccga gatccaccgc atcgcccggc ccggcgccct ggtcgagatc
      961 atcgtgcccc acttctcgaa cccgtacttc tactcggacc cgacgcacgt ccgtccgttc
     1021 ggactgtaca cgatgaacta cttcgtcgac accgagaagc agcccgaggt ctggcgggtg
     1081 cagaccttct acagcaaggc gcgcttcgag atggagtcgg tgaagatcgc gttctaccgc
     1141 ttcaacctct gggatcgcct gttcgtcccg ttcctgcgat acttcgtcaa ccgctcggcg
     1201 cgcacgcaga acttctacga gctgcgcctg agctggctgt tcccggcggc ggagatccgc
     1261 taccggatgc gcgcctgcaa gtaagcgaat gctgcaacgc gtcctgatcg cgtacacctc
     1321 gaagccgccg atcatcaact acctgacggc ggccttcgcc cggcgcggaa tcgaagtgcg
     1381 ggcggtattc gcggacgaga acacctggtt cgaccgtctc gtgatccacc gtctgaacaa
     1441 gctggcgcac aacttccggg tgatccccaa atcgaagaac ttcttcgaga accggcccgc
     1501 gtcacacatg aatttccgca gcgcccggct gcgcgacgcg atctctgcct acgacccgga
     1561 cctggtgttc cacatccgcg gcctggggtt ccgcccgtta gcgttcgagg gggcgcgccg
     1621 gaagttcgcg tggtgggtgg agtccgacga gcgcgtcgac gaagcgcttg ccgaagtccc
     1681 ctggttcgac cgctacttct tcatcaactc ggccaacgtc gaggcggcgc agcgcgcggg
     1741 ctacggccac gccagctacc tcccgcacgc ggtcgacgcc tcggtctttc gcccgcttcc
     1801 cggcgtggtg aaggacatcg acttcgcctt cgtcgggttg tggtcggagc gcaggcagaa
     1861 gttcatcgag gcggcgctcg aggtctccgg taatggcgcg ctgtacggcc cgaagtggta
     1921 tcgcaagacc ttccccgacc ggcgctttcg ccgcatcttc cgggggcgct acatcgacgg
     1981 cgcgccgctg gtgcggctct acaaccgggc gaaagtcgtc ctcaacatca ccacctgggg
     2041 cgcgaacggc ggcgcgaacc gcagcggcat gacgatgcgc acgttcgagg cgccggcgac
     2101 cggcagcctt ctcctgacgg accattccgc agagctgagc ctcgccgtga cgcccggaac
     2161 ccacgtcgac acgttctcgg acctggacga cttcaagaag aagctgcact actacctcga
     2221 tcacgcaccg gaacgccagc ggatcgccgt gcacgggatg cagcacgtcc gcgtccacca
     2281 cacctacgat gccatggtcg attcgatcct cgcggcatac gaggacctgc cgaccggaga
     2341 gcgatagccg tgcacccgtt gtccctttcc gcatgggccg cggctttctt cgtctccgcg
     2401 acgttgtttg cgcacacggt ggcgttccgg ctgctgatgc tcgcctgctg cgccgggctg
     2461 gcactcgtgg cgatcgcccg ggagcgcggc gcgattcgag cgctcccgcc gatctggccg
     2521 gcgctcgcgc tctggacagc ctgggcgctg ctctcgctct cgtggtcgat ggatccggag
     2581 cgcaccgcga aggaactgag gaacgaggtc ggctacaccg ccctggcgtt ctgggcctgc
     2641 tacgtcgccg gccaggcaca ccacgccgtc cgggtcgtcg cgcccgtcgt tggtgcggcg
     2701 gcggtgctgc tgtgcggact cgccttctac tattttccac acgggaatca gtggaatcca
     2761 ccgccctggc ctggcggacc gggaaatctc tcgagtaccc ttctaacgct aatgccgtgc
     2821 gccctcctag gcgcctggta cggccagcgt gcgcgcgacc ggctggtgtc gctagcgagc
     2881 ctcgcgcttg tcgttctgct gttcgccgcc gcctatacga cgctcaaccg gacgatctgg
     2941 gtcgggttcg ccgcgcagtt ccttctcatc ggagcgctgc tcgcctcccg gaaggtgcgc
     3001 ccgatgccct tgcgcgcaaa ggtggtcggc accgcgctcg cgaccctgat cgtggcgagc
     3061 gcggcgctcg cgacgttcca gattcagatg gcgcgccagc aggccatccc cgcggccgcg
     3121 ctcgaccggg actcgcgcat cagcctgtgg ccccaggtgc tcgagcacgt gaaggaacgg
     3181 cccctcaccg gctacggctt cggccgcggc atgctacgca gctcactgct gaaggagttc
     3241 gacgacacct tcctttggca tgcccataac ctgttcctgg ataccgtggt ccaggtcggg
     3301 ctgccgggcc tcgtgctgct gctctttctc cttggagcga cggtgcggga gggctggcga
     3361 ctggcgcgcg cctccgaccg ctttgcggcc gcctgcgggt tcgcgctgct gtgcgtcgtc
     3421 gccgggatgg tgatgcgcaa catgaccgat acgctctggg tgcggcagaa cgcgctcctt
     3481 tactggggag tgctcgggtt gctgctcgcc tggggccaca cggcgcgaac ctcagcgtcg
     3541 aggaggtcta cctagcgtgg cggcggcttg ccgaaaagac tctgcctgcg cgcgcggctt
     3601 cgcctcaggc ctgagctcga ccacgtattc gggtatccag atccgcagct tgtcgcgcac
     3661 ccggtccacg ctcttctcgc cggggctggt gagccactcg accacttccc cgagcaacct
     3721 gccgttctcc ggggcacgcg cctgcgccac gcgcagcttc ggatgcggcg tgggaagggt
     3781 cttctcggcg tcggcgagcg gctcctcgta gagtttctcg ccgggccgca acccggtgta
     3841 ctcgatgcgg atctcgccct cagcgagccc cgacaggcgg atcatctgcc gcgcaaggtc
     3901 gaggatcctt accggctcgc ccatgtcgag cacatagatc tcgcccccct tgcccatcat
     3961 gccggcctgc agcacgagct gggctgcctc gggaatcgac atgaaataac gctggatgtc
     4021 gggatgggtg acggtgaccg ggccgccgcg cgagatctgc tcgcgaaaac gcggcaccac
     4081 gctgccattg gagccaatca cgttgccgaa gcggacgatg gtgaactgcg tgccgctgcc
     4141 ccgctgcagc gcctggcaca ccatctcggc gaggcgcttc gaggcgccca tcacgctggt
     4201 gggattcacc gccttgtcgg tggacacgag gacgaacttc tcggcacccg cgcgctgcgc
     4261 cgagcgcgcc acgcacactg tcgagacgac attgttggcc accccttcga aggcgttgtc
     4321 gtcctccatc agcggcacgt gcttgtacgc cgcggcgtgg aacaccacct gcggccgata
     4381 ccggctgaat acttccgcca cgcggcgctc gtctttggcg tcgcccagga ccgccgcgac
     4441 gcggactgcg gggtgacggt cgcggaactc ctgctcgatg gaatagagcg cgaactccga
     4501 gttgtccagc agcacgagcc gcgcgggcgc gaagcggacg atctgccggc agagctccgc
     4561 cccgatcgag ccgccggcgc cggtgacgag caccgccttt ccgtcgataa aggcgtgcag
     4621 cccggcatcg tcgagttgca ccgggtcgcg cccgagcagg tcgtcgagct cgatcttgcg
     4681 cagcgccgag acgctgaccc ggcctgaaac gatatcggag atcgccggca ccgtcatgac
     4741 cgaaagcccg gcctgcgcgc acaggtcgag ggcgcgccgc cgcgaggtat ggctcgcgcc
     4801 gggcatcgcg atgatggcct gggtgacgcg atgggcgttc gcgatttctc cgacccggtc
     4861 gatcggcccg cggaccttga cgccgaggat ttccgcgccc tgcttgcgcg ggtcgtcgtc
     4921 gagcaggccg acgactcgcc actgcgcgct cgccgcaagc tccttgagga gggtgttgcc
     4981 gaagctcccc gcgcccagca cgagcaccgg ggtcgcggtc ggcttagaca cgatgggcag
     5041 caggcggcct tcgcgccagg cgcggtaggc gagccggctt ccgcccatga cgaggatgag
     5101 aagcatgggc gtcagcacgt agactgtccg cggcacgctc gccccggcgc cggcgacgat
     5161 cagcacggcc ggcaccgcga gcgcgcccac gcctaccgcg atgacgatgc gctggagatc
     5221 cgggaggctg gcgtagcgcc acaggccgcg atagaggccc agcctccaga agaggacggc
     5281 gtagatcgcc gctgcccagg gcagccgctc gagcatcagc tcctggtagc tctcgggaat
     5341 ctcgaagttg aagcgcagcc agaatgccgc gagccaggcc agggcggcgg caacgacatc
     5401 atgagcgaag gcgaagaggc tccggctatt catgcggtct ctcggtcgtg cgcggatctg
     5461 cgccagcgca catcgaccca agcagccaac atgccgagca gcagcagcat tcccaggaac
     5521 accgacgcct gaatcgcggg cggttgctgc cgcgcgagca gcgccgcgat ggcgcacgcg
     5581 agcatgaccg cgtacgcagc caatgccgtc ccgcgatggc ccacgcccat gcgcaccatg
     5641 cgctggtagt agtggtcacg atgcgcgcgc cataccgcct ccatcctgag cagccgcttc
     5701 agtagcgtga gcgtggcgtc tcctatgaac ggcgcgaaga cgagcacggg aaaccacagc
     5761 ggccatatgt cgtcgcgcca gcccaggatg ccgagcgcgt agacgaggaa ccccagcgga
     5821 atcgatccca cgtcgccgag gaaaaggcgt gccgggaaaa agttctgcgt gagaaagccg
     5881 gcgctcgccg cggcaagcgc ggcgcacatc gaggcaagcg gcagatgccc cgccagcccg
     5941 gccgcggccg aatacgcgcc aaagccgatc gcggccatgc cgccggcgag gccgtcggct
     6001 ccgtccatga agttgtagag gttggtgatc cacactccgg cgacgatcag caccgcgagc
     6061 tccaccggat gcatcgggct caatacgtac caggcgaagg ccccgcaggc ggcgaagtgc
     6121 gccagcagcc gcgcaaccgt cggcagcccg cgcaggtcgt cgagaaacga cactgccgcg
     6181 agcgccagcg ccatcgccat gagcggccag agctctcctg cgccgagccc cagagcgagc
     6241 gcggcgccga gcaggattgc gatcccgccg gtgcggggaa ccggccgctc gtgcagcgag
     6301 cgcgcgttcg gctggtcgag cgcgacggag cgcaagcgtg acgcgagcag gcgcccggcc
     6361 gcccatgcca ccacgaacga cgctatcgcg agtcccccga tcacccggcg cattctaacc
     6421 gcggccaaac cacgcggccg tcgcagccag gccttcctgc tgcgaaaacg ccggccgcca
     6481 gtcgagctcg cgccgtatcg ccgtgtcgtc cacttcgagg gagcgggtca gcgcgcgcag
     6541 gccgggaagc gcctcgagca ggcgcgtggg caggcgaaag aggcgcgccg gacgcccgag
     6601 cgcattgccg aggcggcggc acagctcgcc gctggaaagc accgggccgt cggaaaggag
     6661 atacgtgcgc tcgatactcg cctcccgcgc gatgcagcag aggacggcgt cggcgagatt
     6721 acctgcgtaa agaatgctcc tgcggttcgc gacgcccgcg aggggcagcg gcacgccgcg
     6781 cgcgagcgcg ctcatgagag cgcggaagtt cgcctttacg cccgggccgt acatcagcgg
     6841 ggggcgcagc accgtgaggc gcaggcccgc gatccggcgc aggcgctctt cggcgcgggc
     6901 cttcgactcc gcatacggat cgcgcggcgc aacggggcag ccctcatcga gcgggacgtc
     6961 gctctgctcg ccgtgaacct tcacggtgct gacgaagatc atgcgcgccc cggccgcagc
     7021 cgccgcgcgc ccgaccttat cggcgagatc cacgttgaca cgatgcagct cttcgggact
     7081 tgcgccgcgg ccatgggcaa gcgcggcgag gtgaacgacg gtgtccgcct gcggcggaag
     7141 acggacttcg gcgacaccgt gcagcggcag cgccaccggg cgatgcgcgc gggccgcgag
     7201 cgccgggcac agcgttgctc cgacgaatcc tcgtgccccg gtgaccgcga cgatcaacgc
     7261 gggggtttca tccagacgcg gcggttcacg taatcggtgt agctgagaat gattcgaacc
     7321 accttcgccg acacgtcggc ggccttgtag tcgggcaccg ccgcggatct tcgtccttca
     7381 tcgtgatgcc gcctgacgag cgcgatcgcc tcgaggacgt tggccgctcc caggccggac
     7441 atgacgagcg tccccacgtc cattccctcg gggcgctcat gcgcctcgcg cagggtgact
     7501 gccggaaagc cgaggatcga gcattcctcc gtaagcgtcc cgctgtccga cagcacgcaa
     7561 tacgcgctcg actgaagccg cacgtagtcg aagaagccca tcggcttaag gaagcgcacg
     7621 tcggcccccg ccatgtccgt gcctgccgcc tccaggcgct tctgcgtgcg cggatgagtg
     7681 gagaacacgg tgggcagccg gtgccgctgc cacaccgcct gcagcgcccc gacgagcgcc
     7741 ctgaagctcg cctcggcgtc gatgttctcc tcgcggtggg aactcaccac gaagtagcgg
     7801 ccggcctgaa gcttcagccg ctggaggacg tcggaagcct cgatgcgcgc cgcgtagtgg
     7861 ccgagcactt cgcgcattgg cgagccggtc ttgatcacgg tgtccggccg gaggccttcg
     7921 gccagcaggt gccggcgcgc gtgctcggtg tagaccaggt tgatatcgga gacatgatcc
     7981 accaggcggc ggttgacctc ctcggggacg cgctcgtcga agcaccggtt gccggcttcc
     8041 atgtggaaca gcgggatctt caggcgcttc gccgagatcg cggcaaggca gctgttggtg
     8101 tccccgagca gaagcaccgc gtccggggcg agcttctgca gcagcgcgta ggagcgcgcg
     8161 atgacgttgc cgatggtctc gcccacgctc ggtgcggcgg cctccagaaa gtggtcgggc
     8221 cggcggatct cgaggtcctt gaagaagatc tcgttcagct cgtaatcgta gttctggccg
     8281 gtatggacga ggacgtgctc ggcggcgcgg tcgagctcgg cgagcacgcg cgagagcttg
     8341 atgagctcgg gccgcgtgcc gacgatcgtt gcgaccttca tcgcgctagc gcagcagctc
     8401 gcccgccggc gcgtcttcga tcatcagacg gtgtttcttc aggagcgccg cggtgcccgc
     8461 caggtcgatg acggtatctt ccgagctgaa ctccttgtcg agcgcgttgg gttcgttcgc
     8521 gtcgtcgcgg cgcagctcgg gcagcatcgg ccggatgacc aggtattcgc cgcggcgcgc
     8581 gcaatggtgg atctcctctt ccgacaccat gatctcgtgc atcttctcgc cgggccgtac
     8641 gtcgatggtc ttgagcggga ccttgcgttc gccgatcagc gccttggcga ggttgaggat
     8701 cgtggccgaa gacgcccgcg gcacgtacgt ctcgccgcgg cgcgcctcct tcagcgcggt
     8761 gagcacggta tcaaccgcct gctcgaggct gagcagaaag cgcgtcatgc gcggatcggt
     8821 gatcgtgacc gggcccccgg cgcggatctg ctcgtggaag aggggaatca ccgagccgcg
     8881 cgaggcgagc acgttgccgt agcgcacgca gatgaaccgc gtgcgcgcgt tgaggatgtt
     8941 ggcggcaacg aagatgcgct cctgcagcgc cttggtcatg cccatcacgt tgaccggctt
     9001 gcaggccttg tcggtgctga tgccgaccac cgtcgccacc ggcaggcggc cctgctcgat
     9061 cgcctgcacg atgttgcgcg cgccgatgca attggtgagc accgcctgtt ccgg
//
LOCUS       DATBSZ010000079        19125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1991992, whole genome shotgun sequence.
ACCESSION   DATBSZ010000079 DATBSZ010000000
VERSION     DATBSZ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19125
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991992"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VIG70_05280"
     CDS             <1..465
                     /locus_tag="VIG70_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY8250000.1"
                     /translation="AVHDASLIEGLDKRGPEVANEFRRLNPPVVHPAVRLHAESGRKA
                     LYVNERVRNFLGLSEAESKPIVKFLCEHAVQPRHTYRHYWSIGDLVMWDNRCLVHLAV
                     GDYDPAEIRHMIRTSCMGDYYGRYLDPEAAAAQMKSAPVPAADKKSVAALHD"
     gene            complement(462..1436)
                     /locus_tag="VIG70_05285"
     CDS             complement(462..1436)
                     /locus_tag="VIG70_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250001.1"
                     /translation="MMRKTLAALAAALVSFACCAPALAQGYPSQPVKILVGFPPGGTT
                     DVMGRLTAQELGVQTGKPVLVENRPGASGSIAAGVVAKSAPDGHTLIMVPSTYGTASA
                     LYDNLPYTDAELVPVGLIGSTPYVFVVHPSIPVASFSELIRLLKQNPGKYEFASSSPG
                     TAQHLGGELVKRMAGVDMLHVPYKGSGALLPDLLSGRVPMMFENVAIMTPHIKKGSLK
                     PLAISSAKRSALMPEVPTVAETGQGLEGFEVLGWFALMAPAKTPPEVLRFLNAELNKM
                     ITKPAIVTRFQELGAEPMGGTAEQAAAYIRAEQEKWGKVIRDAGIKAN"
     gene            complement(1486..2376)
                     /locus_tag="VIG70_05290"
     CDS             complement(1486..2376)
                     /locus_tag="VIG70_05290"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEY8250002.1"
                     /translation="MKKTTALRRLLKKKEFIYMPVAYDPLGGRLLQSIGFDCVYTGGF
                     VTGASRCTSEPLLTMDEQLRLAGDVAAAIDVPLVVDGGAGFGDPLHTMRTVRECIRAG
                     IAGAHIEDQLYPKRAHYHKYVAHAVPRKEFADKIRFACKQRDRSDPDFVVIARTDTCR
                     FEGLKEAVARVNMAADAGADLGLIFPRNDEEMKKAPKQARIPLVYVISRGNRDGRPLP
                     TAAQLADMGYKAAIDALTYLLASFHFAKLAYREIKRTGGYTGLTQEQCVAARKEIETL
                     VGLDEFYEIEEQTVEKKRPT"
     gene            complement(2384..2794)
                     /locus_tag="VIG70_05295"
     CDS             complement(2384..2794)
                     /locus_tag="VIG70_05295"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8250003.1"
                     /translation="MKKIRKEVINVPGVSKHLKKEGIPLNPVIRAGNFVFVSGLTPMD
                     RRTGKIPLVTIDKQARMVLENMKVCLKAAGSSLDNVLKCTVYITNTAYFDEVNKVYRK
                     YFRKDQLARVFCVVGSWPKKFDVEIDAIALVDQD"
     gene            complement(2791..3774)
                     /locus_tag="VIG70_05300"
     CDS             complement(2791..3774)
                     /locus_tag="VIG70_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY8250004.1"
                     /translation="MLPKVHILGTGGSISGIGPHRLDYTLYPEVGKKLSIEQLLARIP
                     EARELAELSGEDVLQVGSPSIGPAEWLQILQRIETTSAAGIVITHGTATLEETAYFLH
                     LTVKSARPVVVTGAMRPPSAVGTDADLNLLDAIRLAASPEAAGRGVLTVLNNEIQSAR
                     DVSKSNAFRVNTFDSRDFGLLGYVDADARVLFYRSVTRAHTTATPFDVRGRKDLPRVD
                     IVYAYAGADGLLVDALAQHRPAGIVLVGFGGGSFPAAFLDAGKRAVQAGIPVVLATRS
                     WNGRVVTTPKKDADGFIVCDDLMPQKARILLMLALTVTRERGAIQRMFYDL"
     gene            complement(3774..4805)
                     /locus_tag="VIG70_05305"
     CDS             complement(3774..4805)
                     /locus_tag="VIG70_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY8250005.1"
                     /translation="MKVARFHKFGGPEVLVHEEAPKPTPKPGEALVRVRAVGINHVDL
                     DHRAGTSRIPLTFPHILGREFAGEVAGLNGDGAGFKEGDRVWVTCRLPCRKCELCLAG
                     RDNLCLQEGYFGLDIPGGYAEHVAVPIANLNALPSHVRFEDAAAAQIAFGTAWHVLVN
                     RGFLQAGQTVLIQAAGSGIGSAAVQVAKLAGAAAIIATASSEKKLEQAKALGATHLIN
                     YSKESFSEKVMAFTGGRGVDVVMEHIGGEVFTQSLKCIARGGIIVTVGAHAGEVVPFD
                     IIPFFRRELRLSGSKNASVLELRKVMGLVADGKLKPVIHKALPLAQAAEGHRVVDSRD
                     FFGKVILLP"
     gene            complement(4802..6259)
                     /locus_tag="VIG70_05310"
     CDS             complement(4802..6259)
                     /locus_tag="VIG70_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8250006.1"
                     /translation="MFDLVIRGERVVTPHGVGPWEIAVKDGVIAALASPGTFPAAQAA
                     RVIEAGRALVMPGGIDPHVHCAWYIPPMRPGDPPGTSGPPEQVSRAALWGGTTTLIDF
                     AACKPDRAGDESSSQSVRDAIEARDKDWAGKCYCDYAYHVMLRGKVPPATIEELKEAV
                     QAGYATVKIFTTDITPSRAGRMIRFGEIWEIFKVLAREGGLAVIHAEDDDIVRHMYEK
                     LFRENRSGFEHMAEVHNALSEDLSFRRVIRLAENVEGMALYMMHVSAASGVDAIAESR
                     ARGYPIYGETLHQYLLYTSEDYKKPNGQIYHTYPSLKSKRDQQGLWDGLQNGSLHCVG
                     TDEVCCNLSVKVRGSRIDDTTGGNAGVEPRVAVMYTETVEKRGYSLEKFVDVVSSNAA
                     RILGLYPRKGAIAVGSDADIVVLDTSQRRTVRAQDLHEADYSPWEGKEVGAWPSVTVL
                     RGKVMVADGKFHGDLKDGQYLKRKIPPEILKRGSS"
     gene            complement(6244..7206)
                     /locus_tag="VIG70_05315"
     CDS             complement(6244..7206)
                     /locus_tag="VIG70_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8250007.1"
                     /translation="MSQLPISVVTGFLGSGKTTLLSRLLRDPAAARTAVIVNEFGEVG
                     LDHLLLESSDEEIVLLEGGCLCCRVRGDLVRTAGDLLARRARGEAAFDRIVVETTGLA
                     DPAPILQALMTDREIADALRLESVIATVDCAAGDATLDAHPESVKQAALADRIVLTKS
                     DLADPAASGLAERVRRINPGAPQLVALHGAVDASLLFNSSVYEPRLYESHAHSGEVTT
                     FCLRRSAPLHAVTLSLFLQLIAEHCGDRLLRLKGLVDVVESPGRPAVIHGVQHVFHPP
                     AWLDAWPDEDHSTRIVVISRGLDAQWLQDLLEVLDDEVKACSIS"
     gene            complement(7203..7496)
                     /locus_tag="VIG70_05320"
     CDS             complement(7203..7496)
                     /locus_tag="VIG70_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250008.1"
                     /translation="MSSHKTDRLGRVLSGALRVWAVSARVEHDPEGAFLVRTSSGTTM
                     RIVPAAPYGWCVWREGHPLGVHAGLPGLLRSLREELAPEAARGRLIIGSQSLI"
     gene            7632..8657
                     /gene="dctP"
                     /locus_tag="VIG70_05325"
     CDS             7632..8657
                     /gene="dctP"
                     /locus_tag="VIG70_05325"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY8250009.1"
                     /translation="MNHKLPLLLSVFGCALGLAAGDAGAQAKFNLRWGHYLADSPFVQ
                     LEKDFAAAIEKRTNGQVKITITFAQGLGKTTELLTLTAKGAIDMTATAPGYNPDQLHY
                     WRAFQLPLTFETSKQAMHVLGVVVKEFPIYRQEMDRAGVEWLFQQPLGEYYLSGPSPE
                     CDSMASLKGKKIRSFGADIPKAFTAIGAVPVTVAPTDIYEALQRGTLDYSFINAGNMQ
                     AFKLVEVAKQHCGPVMAITGHNITISKRTWARLPKDIQQVFREQSVKTAQDYLAWVGD
                     FETKAAAGMKSQGATFKPFPAAELKKWRSSAPDFLAAWAEDTAKATGDAATPKKVAAR
                     WRELISK"
     gene            8658..9188
                     /locus_tag="VIG70_05330"
     CDS             8658..9188
                     /locus_tag="VIG70_05330"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY8250010.1"
                     /translation="MQTLSAWLSRLALGLLLLGTVGMIASMLIGVADVVGTKFFDWPV
                     PGTLEFTESTMVLVVFGALAYAQGSRSHIRVELLYSHTGPRGQSFMEAVTHIVAFVFF
                     VLVAWQGLSELSYSWELKEATMGTVRFPLYPARFFLLLGVGLLLVRLALDIVVDIGRM
                     RRGEAPPVAAAPEQRE"
     gene            9192..10511
                     /locus_tag="VIG70_05335"
     CDS             9192..10511
                     /locus_tag="VIG70_05335"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY8250011.1"
                     /translation="MELVSPQVAGWIVVGLLLLWLTLGVHIGVALGLTGFIGIYLTVG
                     PDAALAQIGTIPFSTTNSFALAVIPLFILMGSFATVAAITTDLFRAAYVWLGNLRGGL
                     AMATVMSSAAFGAASGSTIVNAAVFTRMAMPEMTRFGYDVRLSSGCIAAAGTLAALIP
                     PSILMVVYAVITEQSIGKLLIAGIVPGILTAAIYCMGIYVLARWRPDLAPLASIAFTW
                     KERFRSLYSVYGIIILFALVVGGIYGGYFPATYAGAVGAFGAFVIALVKGRLGIKSLV
                     EVLKDAAVTTSVIFIIVIGGIIFARFLTYSGLVDIISHGLLGLGTGKYVYLLGFIVLF
                     IVLGCFIEPIAIMVMTLPIMFPVMVKAGFDPIWLGVVAVKLAEISVLTPPVGLNVFVV
                     KSSSPVAVTLGQVFAGVTPFILLDFLSLVLYVLFPEMLLWLPNLMKS"
     gene            10525..11925
                     /locus_tag="VIG70_05340"
     CDS             10525..11925
                     /locus_tag="VIG70_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8250012.1"
                     /translation="MAVLKAQGKTFPTTTEVLIIGAGACGTIAALAAKERGAEVVILE
                     RDAKPSGSTSLSSGQIPAAGTKLQRSAGLLDDTPELLYKDILAKAHGECDHAIARLIA
                     EESRNTVEWLAERHQVPLSCVLNFQYPGHSRPHMHAAKSLDGAELHAALLAAVERHEI
                     ALATAAHATDLYADGDGRVRGVRVTRPDGKTEDIGCEALILACSGYGGNKEMLKKYIP
                     DAAELFYEGHVGNQGDAVRWGEALGASIKDMGSFQGHGAMATPHGVHLNWATVTEGGF
                     HVNLDGRRFSNENAGYSEQALKVHRQPGGVAWAIWDERSDAIAIQQASHKEAKDAGAI
                     KRCDAVEAIARVVGCDPQALAQTFADVAAAIRGEKQDPFGRDFKGKPPLKPPYCVSKI
                     AGALTHTQGGLEVNTQMRVLRKDGTPLPNLFAGGGAARGLSGPSDWGYLSGSGLMTAT
                     SMGRLAGIAAAKLVKG"
     gene            11928..12614
                     /locus_tag="VIG70_05345"
     CDS             11928..12614
                     /locus_tag="VIG70_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8250013.1"
                     /translation="MIKFYYSTAPNPMKVALCLEEMGLAYEPIPVDTRKGAQHEPGYL
                     AINPNAKVPSIVDGDVTMFDSNAIVLYLAEKTGKFLPKKAQERGPMLSWLMFVASGIG
                     PFTGQYVHFKNYAPEKIAYALNRYEFEALRHWRIVDAHLAKSKYMVGDIYTVVDMALW
                     GWARLLSNILGEQAAKFANVKRLVDEISARPAAQKAVALKDRYTFKTEMDDEAKRAMF
                     PGNYRAQAGA"
     gene            12647..13510
                     /locus_tag="VIG70_05350"
     CDS             12647..13510
                     /locus_tag="VIG70_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY8250014.1"
                     /translation="MITLGAAQTGPVNEDDWRPNVPGACAMIEEAGRRKVDILSFSEL
                     FLTPFFPNRLTRDYDKFFVTLDEPELAPIFKAAVDSRVALVFPFGEKNGRHYHNSCLV
                     TDKNGKRLGIYRKTHIPAILPSNLPGGTGSYEKFYFSPGTEFPVFDLCGTKIGIQICY
                     DRKYPEGSRLLALKGAEILFMPICAATYGETKLRGNTWELPLQARAYENGVYVVAVNR
                     CGMEQGRNHIGRSMIVDPIGADMLAVASSDTPEVLVATVDLDNVAAAQTSLPWWRDRR
                     PELYASLAAAV"
     gene            complement(13507..14877)
                     /locus_tag="VIG70_05355"
     CDS             complement(13507..14877)
                     /locus_tag="VIG70_05355"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY8250015.1"
                     /translation="MTLARELVRAARAAASTPLPEDVATAARLHFLDAIGVGLASAGS
                     PIGAAYRGFAADVVKGGPATVLGQSTAAGAADAALINGGLIHSLEFDDTHTASIAHGS
                     AVLAAGALAVAEAQNASGAALLGAYARGWEVLIRFGLAAPNGFHAKGFMSTSVTGTLA
                     VALLAAELMGLDEERTVAAVGIALSQASGVMEFLTNGSSVKSLHPGWAAHGGVVAAML
                     ARSGMTGPETSLDGKHGLFRQFTGDEAAAGRFRALIGGIGREWHLPKAAYKFYPCCHY
                     LHPFIEAAGKLAERGVRPGDVERIVCRVPQGEAAVICEPWERKQAPDNGHAARWSLPI
                     AVAAQLVEGKVDLATFEAPAGAAVRQLAQRIAWEPLPGARFPQRFEAEVVCKTKTETQ
                     TIRIDDVFGNHTRPPGSEAVLAKFRANAARSLKSDAVGALERAAQDLGAASDLKALSA
                     ALRVTH"
     gene            complement(14874..16262)
                     /locus_tag="VIG70_05360"
     CDS             complement(14874..16262)
                     /locus_tag="VIG70_05360"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY8250016.1"
                     /translation="MTPNENPARAQAAANTRKLIEWAVEAVRKPLPEPVRRRAAIILS
                     DDIGAMVAGSLETQVAKAREVLLRTSSGKSEATVLAKGAPRADRYAAASANGMAITWC
                     ELDEGFRNASCHGGAYTIPALLAESEAQGRTVEEVLRALAIAYEVTTRFALAFPFPVF
                     NVHPHAAFAAIGGAAAAALARKQDAKTMLAAVTGGASMSFAGPFDTAVEGALIRNAWT
                     SAGAWLGLRAADWAEAGIGGNDVTPYDVFVGAYKTNAKPDALVAGLGDTWSVTNGYHK
                     VFACCGYAHSAVEATLELLGRMQRKKEEIAEIVVETAPGGQALRTVEPETVLAAKFSL
                     PHAIAATVQLGTAGARAFTQDKLEDAEISKLRTRVRLQPYPDIKPAPNDRPARVTLRF
                     GDGAEMSAVVESARGGADKPFDEPTLLSKLAENAGSVFPAIPETMKKVIAGDSELLSQ
                     SWRDALARMVKA"
     gene            16318..17148
                     /locus_tag="VIG70_05365"
     CDS             16318..17148
                     /locus_tag="VIG70_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY8250017.1"
                     /translation="MRIWHQSFTDLTVMPLYRKTITEHAAAVMGQDAAVSVHGLRPGT
                     YTEGCAPIDAIKYRYVATVHELQVCDAALVAQREGFDAFALGCFFDPGLRAARSLVDI
                     PVVGLGETCALVACSLARKFGLVTLCEDQSADYTDLMLAYGLERRFAGALALDPPIDE
                     FALEADEKAARAIEERFDVACAAMVARGAEIIIPADGVLNEFLVRRRRLTARGGVPVM
                     DSLGTLFQHAAFLVRLARTTGTGISRHQLFAKPSRAMVEHVRGFSRQPELKSDFSGDR
                     "
     gene            complement(17153..18538)
                     /locus_tag="VIG70_05370"
     CDS             complement(17153..18538)
                     /locus_tag="VIG70_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEY8250018.1"
                     /translation="MSDFVLRGGTLVFPDREPEKQDVLVKGGKIEKLLAPGAPTQGVA
                     EQSAAGLHVFPGLIDCHVHFGMGEKITEYSTETANAAQGGFTTVLGYFLNNEAYGDVF
                     RRELEHAIARAHVDFGFHFSTANELHIKELGEYVRDYGVTSFKYFMNFKGEEGRYLGL
                     DGTDDGFFYDLLKESARVGRPIVVCHTENIEIVNRERRKIQAAGGTTLKDWSASKPAI
                     TESEPALRAMYLAEELGARVYFPHISSRKALNVIRHWRQRFQDVIVETCPHYLTHTED
                     SDLGGMGKANPPFRTKDDREALWEAIFDGTVDLIASDHNARKKLNKDKPLWLASQGFP
                     ATASMLSVMLSEGYHKRKLPLQRICQLLGSVPAKVFDLAPLKGSLSPGADADFTLVDL
                     NKERVLHAADFASYADYNLHEGWNLKGWPVRSISRGETVMDNHKIVGKGGHGKYLWRR
                     IGQAPIPGKTR"
     gene            complement(18535..>19125)
                     /locus_tag="VIG70_05375"
     CDS             complement(18535..>19125)
                     /locus_tag="VIG70_05375"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8250019.1"
                     /translation="SSRLQYPELDKQVAMAERIVVTKKDLADPGPVIAFLKEMNPHAE
                     LVDAISRIFSTHGKARKPVASIATLGPIRNREHFSGIRAYAFRPPSPVTWAGWSAWSR
                     LVAAEFGARLIRVKGLLKMEDTGEIAFVQGVQGVFHAPQRFDGWPDDDQANRLVCIAR
                     DLNEQDIAATLPALNTPAGTQAVYSLKDLQERQAQQ"
BASE COUNT         3021 a         6353 c         6496 g         3255 t
ORIGIN      
        1 gcggtgcacg acgcgagcct gatcgagggc ctggacaagc gcgggccgga agtggccaac
       61 gagttccgcc gcctcaatcc gccggtcgtg catccggcgg tgcggctaca tgccgaatca
      121 gggcgcaagg cgctctatgt gaacgagcgc gtgcgcaact tcctcgggtt gtcggaggcc
      181 gagagcaagc cgatcgtcaa gttcctgtgc gagcacgccg tccagccgcg tcacacctac
      241 cggcactact ggagcatcgg cgacctggtg atgtgggaca accgctgcct ggtgcacctc
      301 gcggtcggcg actacgatcc cgcagagatc cggcacatga tccgcacgtc ctgcatgggc
      361 gactactacg gccgttacct cgacccggaa gctgccgccg cgcagatgaa gagcgcgccg
      421 gtgccggctg ccgacaagaa gtccgttgcc gcgctacacg actagtttgc tttgatcccg
      481 gcgtcgcgga tcactttgcc ccacttctcc tgctccgcgc ggatgtaggc cgcggcctgc
      541 tccgccgtgc cgcccatcgg ctcggcgccg agctcctgga agcgtgtcac gatcgccggc
      601 ttggtgatca tcttgttgag ctccgcattg aggaagcgca atacttcggg tggtgttttc
      661 gccggcgcca tcagggcgaa ccagcccagc acctcgaagc cctccagccc ctgaccggtt
      721 tccgcaaccg tcggcacctc gggcatcagc gccgagcgct tcgcgctcga gatcgcgagg
      781 ggcttgagcg agcctttctt gatatgcggc gtcatgatcg ccacgttctc gaacatcatc
      841 ggcacgcggc ccgagagcag gtcggggagc agcgcgccgc tgcccttgta gggcacgtgc
      901 agcatgtcga cgcccgccat acgcttcacc agctcgccgc cgaggtgctg cgcggtgccg
      961 gggctggagg aggcgaactc gtacttgccc gggttttgct ttagcaggcg gatcagctcg
     1021 ctgaagctcg ccaccgggat cgaggggtgc acgacgaaca catagggggt gctgccgatc
     1081 aggcccacgg gcacgagctc ggcgtcggtg tagggcaggt tgtcgtagag cgcgctcgcg
     1141 gtgccgtagg tgctcggcac catgatcagc gtgtggccgt cgggcgcaga cttggccacc
     1201 acgcccgcgg cgatggagcc gctggcgccc ggccggtttt ccaccaggac cggcttgccg
     1261 gtctgcactc cgagctcctg cgcagttagc cgccccatca cgtccgtagt gccgcccggc
     1321 gggaagccga cgaggatctt caccggctgg cttgggtaac cctgcgccag cgcaggggcg
     1381 cagcacgcga acgagaccag cgctgcggcg agggccgcca gtgtttttct catcatgatt
     1441 tctcctccag tcgggctgct gaaatgggca gacagcgcca ccatcttacg taggtctctt
     1501 cttctcgacc gtctgctcct cgatttcgta gaactcgtcc aggccgacga gcgtttcgat
     1561 ttctttcctg gcggcgacgc actgctcctg cgtaaggccg gtgtagcctc cggtacgctt
     1621 gatttcccgg taggcaagct tggcaaagtg gaagctcgct agcagatacg tgagcgcgtc
     1681 gattgccgcc ttgtagccca tgtcggcgag ctgcgccgcg gtcggcagcg gccggccgtc
     1741 gcggtttcct cggctgatga cgtagacgag cgggatgcgc gcctgcttgg gcgccttctt
     1801 catctcctcg tcgttgcgcg ggaagatgag accgaggtcg gcgccggcgt ctgccgccat
     1861 gttgacccgc gcgacggctt ccttcagtcc ttcgaagcgg caggtgtcgg tgcgcgcgat
     1921 gacgacgaag tccggatcgg accggtcgcg ctgcttgcac gcgaaccgga tcttgtcggc
     1981 gaactccttg cgcggcacag cgtgcgcgac gtacttgtgg tagtgcgcgc gcttgggata
     2041 gagctggtcc tcgatgtgcg cgcccgcgat gccggcgcgg atgcactcgc gcacggtgcg
     2101 catcgtgtgc agcgggtcgc cgaagcccgc gccgccgtcg accaccagcg gaacgtcgat
     2161 cgccgcggcc acgtcgcccg ctaggcgcag ctgctcgtcc atggtgagca gcggctcgga
     2221 ggtgcagcgc gaggcgccgg tgacgaagcc gccggtgtag acgcagtcga agccgatgct
     2281 ctgcagcagg cggccgccga gcggatcgta ggcgaccggc atgtagatga attccttctt
     2341 cttcagcagc cggcgcagcg cggtggtctt cttcatgcct tccctagtcc tggtccacga
     2401 gcgcgatggc gtcgatctcc acgtcgaact tcttcggcca cgagccgacc acgcagaaca
     2461 cgcgcgccag ctggtccttc ctgaagtact tccggtagac cttgttcacc tcgtcgaagt
     2521 aggcggtgtt ggtgatgtag acggtgcact tcagcacgtt gtcgagcgag gagcccgccg
     2581 ccttgaggca gaccttcatg ttctcgagca ccatccgggc ctgcttgtcg atggtgacga
     2641 gcgggatctt gccggtgcgc cggtccatcg gcgtgaggcc cgagacgaag acaaagttgc
     2701 cggcgcggat caccgggttg agcgggatgc cttccttctt cagatgcttg ctcacgcccg
     2761 gaacgttgat gacttccttc cttatctttt tcataggtcg tagaacatcc tctggattgc
     2821 gccgcgctcg cgcgtgacgg taagcgccag catgagcagg atgcgcgctt tctgcggcat
     2881 caggtcgtcg cagacgatga agccgtcggc gtccttcttc ggcgtggtca cgacgcggcc
     2941 gttccaggag cgggtggcga ggacgaccgg gatgccggcc tgcaccgcgc gcttcccggc
     3001 gtccaggaaa gcggcgggaa acgagccgcc gccgaacccc acgagcacga tgcctgcggg
     3061 acgatgctga gcgagcgcat cgaccagcag gccatcggcg ccggcatagg cgtaaacgat
     3121 gtcgacgcgc ggcaggtcct tcctgccgcg cacgtcgaac ggcgtcgcgg tggtgtgcgc
     3181 gcgcgtgacg ctgcgataga aaagcacgcg cgcgtccgca tcgacgtagc ccagcaggcc
     3241 gaagtcgcgg ctgtcgaagg tgttcacgcg gaacgcgttc gacttgctga cgtcgcgcgc
     3301 gctttgaatc tcgttgttga ggacagtgag cacgccgcgg ccggcggcct cgggcgaggc
     3361 ggcgagccgg atggcgtcga ggagattgag gtcggcgtcg gtgccgaccg cgcttggcgg
     3421 ccgcatggcg cccgtgacca cgaccgggcg cgcggatttc accgtgaggt gcaggaagta
     3481 cgcggtctcc tcgagcgtcg cggtgccgtg ggtgattacg attcctgcag cggacgtcgt
     3541 ttcgatccgc tgaagaatct gaagccactc cgctgggccg atcgacgggc tgcccacctg
     3601 cagcacgtct tcgccgctca gctcggcgag ctcgcgcgcc tccggaatgc gggcgagcaa
     3661 ctgctcgatg ctcagtttct ttccgacctc cggatagagc gtgtagtcga gccggtgcgg
     3721 cccgatgccg gagatgctgc cgcccgtgcc gaggatgtgg actttcggca gcatcaggga
     3781 agcaggatca ccttgccgaa aaagtcgcgc gagtcgacca cgcggtgccc ttcggccgcc
     3841 tgcgcgagcg gcagcgcctt gtggatgacg ggcttcagct tgccgtcggc gacgaggccc
     3901 atgacttttc tcagctccag caccgaggcg ttcttcgacc cggagagccg cagctcgcgg
     3961 cggaagaacg ggatgatgtc gaagggcacc acctcgcccg cgtgcgcgcc caccgtgacg
     4021 atgatgccgc cgcgcgcgat gcacttgagg ctctgcgtga agacctcgcc gccgatgtgc
     4081 tccatgacca cgtcgacgcc gcgccccccg gtgaaggcca tgaccttctc ggagaaactc
     4141 tccttgctgt agttgatgag gtgggtcgcg ccgagcgctt tcgcctgttc gagcttcttc
     4201 tcgctgctcg ccgtggcgat gatcgccgcc gcgccggcga gtttcgccac ctgcacggcg
     4261 gcgctgccga tgcctgaccc cgccgcctgg atgagcacgg tctgccctgc ctgcaggaag
     4321 ccgcggttga cgagcacgtg ccacgcggtg ccgaacgcga tctgcgccgc cgccgcgtcc
     4381 tcgaagcgga cgtgcgaggg cagggcgttg aggttggcga tcggcacggc gacgtgctcg
     4441 gcgtagcccc cggggatgtc gagaccgaag tagccctcct ggaggcacag gttgtcgcgc
     4501 ccggcgaggc acagctcgca cttgcggcac ggcaggcggc aggtgaccca gacgcggtcg
     4561 ccctccttga agcccgcgcc gtcgccattc aggccggcga cttcgcccgc aaattcgcgg
     4621 ccgaggatgt gcgggaaggt gagcgggatc cgcgacgtgc ccgcgcggtg gtcgagatcg
     4681 acatggttga tgcccaccgc ccgcacgcgc accagcgcct caccgggttt cggcgtgggc
     4741 ttgggcgctt cctcgtggac caggacctcg gggccgccga atttgtggaa ccgcgcgacc
     4801 ttcatgaaga cccccttttc agaatctcag gcgggatctt gcgcttgagg tactggccgt
     4861 ccttgagatc gccgtggaac ttgccgtcgg cgaccatcac cttgccgcgc agcacggtaa
     4921 ccgacggcca ggcgccgact tccttgcctt cccacggcga gtagtcggcc tcgtgcaggt
     4981 cctgcgcgcg caccgtgcgg cgctgactgg tatcgagcac cacgatatcg gcatcgctgc
     5041 ccaccgcgat ggcgcccttg cgtggataga ggcccagaat gcgcgccgcg ttggaggaga
     5101 cgacgtcgac gaatttctcc agcgagtagc cgcgcttctc gaccgtctcc gtgtacatca
     5161 ccgccacgcg aggctcgacg ccggcgttgc cgccggtcgt gtcgtcgatg cggctgcctc
     5221 gcaccttgac cgaaaggttg cagcacacct cgtccgtgcc gacgcagtga aggctgccgt
     5281 tctgcagccc gtcccagagg ccctgctggt cgcgcttcga cttgagcgaa gggtaagtgt
     5341 gatagatctg gccgttcggc ttcttgtagt cctcgctcgt atacagcagg tactggtgca
     5401 gggtttcgcc gtagatcggg tagccgcgcg cgcggctctc ggcgatcgca tcgactcccg
     5461 acgccgcgct tacgtgcatc atgtagagcg ccatgccttc gacgttctcg gcaaggcgga
     5521 tgacgcgccg gaacgagagg tcttccgaaa gcgcgttgtg cacctcggcc atgtgctcga
     5581 acccggagcg gttctcgcgg aacagcttct cgtacatatg cctgacgatg tcgtcgtcct
     5641 cggcatggat gaccgcaagc ccgccttcgc gcgcgagcac cttgaagatc tcccagatct
     5701 cgccgaagcg gatcatccgg ccggcgcggc tcggcgtgat gtcggtggtg aagatcttga
     5761 ccgtggcgta gccggcctgg accgcctcct tgagctcctc gatggtggcg ggcggcacct
     5821 tgccgcgcag catgacgtga tacgcatagt cgcagtagca cttgccggcc cagtccttgt
     5881 cgcgcgcctc gatggcgtcg cgcacgctct ggctggaaga ctcgtcgccg gcgcgatccg
     5941 gcttgcacgc ggcgaagtcg atcagcgtgg tggtcccgcc ccacagcgcg gcgcggctga
     6001 cctgctcggg cgggccgctg gtccccggcg gatcgccggg acgcatcggc gggatgtacc
     6061 aagcgcagtg cacgtgggga tcgatgccgc cgggcatgac gagcgcgcgg cccgcctcga
     6121 tgacgcgcgc cgcctgcgca gcgggaaagg tgccgggcga tgcgagcgcc gcgatgacgc
     6181 catccttgac ggcgatttcc caggggccga cgccatgcgg ggtgacgacg cgctcgccgc
     6241 ggatcacgag atcgaacatg ctttcacctc atcgtccaga acttcgagca ggtcctgcag
     6301 ccattgcgca tccaggccgc gcgagatcac gacgatgcgg gtgctgtgat cctcgtcggg
     6361 ccacgcgtcg agccacgcgg gcgggtgaaa cacatgctgc acaccgtgga tcaccgccgg
     6421 gcggccggga ctctcgacga cgtcgaccag gcccttcagg cgcagcagcc tgtcgccgca
     6481 atgctcggcg atgagctgca ggaagaggct caacgtgacc gcgtgcagcg gcgcgctgcg
     6541 gcgcaggcag aaggttgtca cctcgccgga atgggcgtgc gattcgtaga gtcgtggttc
     6601 ataaaccgaa gaattgaaaa gcaggctcgc gtcgactgcg ccgtgcaacg cgacgagctg
     6661 gggcgctccg ggattgatcc tgcgcacgcg ctccgcgagg ccgctcgccg ccgggtcggc
     6721 gaggtccgac ttggtgagca cgatgcggtc ggcgagcgcg gcctgcttca ccgactcggg
     6781 atgcgcgtcc agcgtggcgt cgccggcggc gcagtccacg gtggcgatga ccgattccag
     6841 ccgcagcgca tccgcgatct cgcggtcggt catcagcgcc tgcaggatcg gcgcgggatc
     6901 ggcgaggccg gtggtctcga ccacgatgcg gtcgaaggcc gcttcgccgc gcgcccgccg
     6961 cgcgagcaga tcgcccgcgg tgcgcaccag gtcgccgcgc accctgcagc acaggcagcc
     7021 gccttcgagc agcacgattt cctcgtcgct cgattccagc agcagatggt cgaggcctac
     7081 ttcgccgaac tcgttgacga tcaccgcggt gcgcgcagcc gcgggatcgc gcagcaggcg
     7141 cgaaagcagc gtggtcttcc cgctgccgag gaagccggtg accacgctga tcggcagctg
     7201 gctcatataa gggactgcga gccgatgatc agccggccgc gggcagcctc gggcgcgagc
     7261 tcctcgcgca gcgagcgcag caggccgggc aggccggcat gcactccaag ggggtgccct
     7321 tcgcgccaca cgcaccatcc ataaggcgcg gcgggcacga tgcgcatcgt cgtgcccgaa
     7381 gacgtacgca ccaggaatgc gccttcgggg tcgtgctcga ctcgggccga gaccgcccac
     7441 acgcgcagcg cgccggagag cacgcggccg agccggtcgg ttttatggct tgacatttac
     7501 tttgagcagc gaaactatga cagcgaaaga tagatgttgc attgggtatt tgcgagatcg
     7561 atatcaaata tcgctgacgt cgataccggc gccggcaaag tgtgagcgca ttccctgagg
     7621 agggaggatc gatgaaccac aagcttccgt tgctgctttc cgtattcggc tgcgccctgg
     7681 gcctggccgc cggcgacgcc ggcgcacagg cgaaattcaa cctgcgctgg ggccattatc
     7741 tggccgacag cccgttcgtg cagctcgaga aggatttcgc agccgcgatc gagaagcgca
     7801 ccaacgggca ggtgaagatc accatcacct tcgcgcaggg cctgggcaag acgaccgagc
     7861 tcctcacgct gaccgccaag ggcgccatcg acatgaccgc gacggcgccg ggctacaacc
     7921 cggaccagct ccactactgg cgcgcgttcc agctgccgct caccttcgag accagcaagc
     7981 aggcgatgca cgtcctcggc gtcgtggtga aggagttccc catctaccgg caggagatgg
     8041 accgggccgg cgtggagtgg ctcttccagc agccgctcgg cgagtactac ctcagcggcc
     8101 cctcgcccga gtgcgacagc atggcgagcc tgaagggcaa gaagatccgc agcttcggcg
     8161 cggacattcc gaaggcattc accgccatcg gcgcagtgcc ggtgacggtg gcgcccaccg
     8221 acatctacga ggcgctgcag cgcggtaccc tcgactattc gttcatcaac gccggcaaca
     8281 tgcaggcgtt caagctggtg gaggtggcaa agcagcactg cggaccggtg atggcgatca
     8341 ccggccacaa catcacgatc agcaagcgca cctgggctcg cctgccgaag gacatccagc
     8401 aggtgttccg cgagcagtcc gtcaagaccg cgcaggacta cctcgcctgg gtcggcgatt
     8461 tcgagaccaa ggccgccgcg ggcatgaagt cgcagggggc gacattcaag ccgttccccg
     8521 ccgcggagct gaagaagtgg cgctcgagtg cgcccgattt cctcgccgcc tgggccgagg
     8581 acaccgccaa ggcgaccggc gacgcggcca cgccgaagaa ggtggcggcg cgctggcggg
     8641 agctgatctc caagtagatg cagacgctca gcgcgtggtt gtcgcgcctc gcgctgggcc
     8701 tgctgctgct cggcaccgtg ggcatgattg cctcgatgct gatcggcgtc gcggacgtgg
     8761 ttggcacgaa gttcttcgac tggcccgtgc ctggcacgct cgaattcacg gagagcacca
     8821 tggtgctggt ggtgttcggc gcgctcgcct acgcgcaggg gagccgctcg cacatccgcg
     8881 tcgagctgct ctatagccac accggcccgc gcggccagtc gttcatggag gcggtgacgc
     8941 acatcgtcgc attcgttttc ttcgtgctcg tcgcatggca ggggctgagc gagctcagct
     9001 acagctggga gctgaaggag gcgaccatgg ggacggtgcg cttcccgctc tacccggcgc
     9061 gcttcttcct cctgctcggg gtggggctgc tgctcgtgcg cctcgcgctg gacatcgtgg
     9121 tcgacatcgg acgcatgcgg cgcggcgaag cgccgccggt cgcggccgcg cccgagcaaa
     9181 gggagtaagg cgtggagttg gtgtcgccgc aggtcgcggg ctggatcgtg gtgggcctgc
     9241 tcctcctgtg gctcacgctc ggcgtgcaca tcggcgtggc gctcggcctg accggattca
     9301 tcggcatcta cctcaccgtg gggccggatg cggcgctcgc gcagatcggc accatcccgt
     9361 tcagcaccac caactcgttc gcgctggcgg tgattccgct gttcatcctg atgggctcct
     9421 tcgcgaccgt ggcggcgatc accacggatc ttttccgcgc cgcctacgtc tggctcggca
     9481 acctgcgcgg cgggctcgcc atggcgacgg tgatgtcctc cgccgcattc ggcgcggcct
     9541 ccgggtcgac gatcgtcaat gcggcggtat tcacgcgcat ggcaatgcct gaaatgacgc
     9601 gcttcggcta cgacgtgcgc ctgagctccg gctgcatcgc cgccgccggc acgctcgccg
     9661 cgctcatccc gccgagcatc ctgatggtgg tgtacgcggt gatcaccgag cagtcgatcg
     9721 gcaagctgct catcgccggc atcgttcccg gcatcctcac cgcggcgatc tactgcatgg
     9781 gcatctatgt cctcgcccgc tggcggccgg accttgcgcc gctcgccagc atcgccttta
     9841 cgtggaagga gcgcttccgg tcgctctaca gcgtatacgg catcatcatc ctctttgcgc
     9901 tggtggtggg cggcatctac ggcggctact ttcccgccac ctacgcgggc gcggtcggcg
     9961 ccttcggcgc cttcgtgatc gcgctggtca aggggcggct gggcatcaag tccctggtcg
    10021 aggtgctgaa ggatgcggca gtgacgacct ccgtcatctt catcatcgtg atcggcggca
    10081 tcatcttcgc gcgcttcctc acctactcgg gcctggtgga catcatctcg cacgggctgc
    10141 tcggcctcgg cacggggaaa tacgtgtacc tgctcggctt catcgtgctc ttcatcgtgc
    10201 ttggatgctt catcgagccg atcgcgatca tggtcatgac gctgccgatc atgtttccgg
    10261 tgatggtcaa ggccggcttc gatccgatct ggctcggcgt cgttgcggtg aaactggccg
    10321 agatcagcgt gctcaccccg ccggtcgggc tcaacgtatt cgtggtgaag agctcctcgc
    10381 cggtggcggt gaccctcggc caggtattcg ccggcgtgac gcccttcatc ctgctcgact
    10441 tcctttccct cgtgctgtac gtgctcttcc cggagatgct gctgtggctg ccgaacctga
    10501 tgaagtccta gctggagttt cgaaatggcg gtactgaagg cccaaggcaa gactttcccc
    10561 accaccaccg aggtgctgat catcggcgcc ggcgcgtgcg gcaccatcgc cgcgctcgca
    10621 gcgaaggagc gcggcgcgga ggtggtgatc ctcgagcgcg acgcgaagcc ctcgggcagc
    10681 acctcgctgt cgagcgggca gatcccggcg gcgggaacca agctgcagcg ctccgcgggg
    10741 ctgctcgacg acacgccgga gctcctctac aaagacatcc tggccaaggc gcacggcgaa
    10801 tgcgaccacg cgatcgcgcg cctcatcgcc gaggagtcga ggaacacggt cgagtggctg
    10861 gccgagcggc accaggtgcc gctttcctgc gtgctcaact tccagtatcc cggccacagc
    10921 cgcccgcaca tgcacgcggc gaagtcgctc gacggcgcgg agctgcacgc ggcgctcctc
    10981 gcggccgtgg agcgccacga gatcgcgctc gcgaccgcgg cgcacgccac cgacctctat
    11041 gccgacggcg acggccgcgt gcgcggcgtg cgcgtcacgc gccccgacgg caagaccgag
    11101 gacatcggct gcgaggcgct catccttgcg tgcagcggct acggcggcaa caaggaaatg
    11161 cttaagaaat acatccccga cgcggccgag ctgttctacg aaggacacgt gggaaatcag
    11221 ggcgacgcgg tgcgctgggg cgaggcgctg ggcgcatcca tcaaggacat ggggtcgttc
    11281 cagggccacg gcgcgatggc cacgccccac ggcgtgcacc tcaactgggc cacggtcacc
    11341 gagggcggct tccacgtgaa cctggacggc aggcgcttct cgaacgagaa cgccggctac
    11401 tcggagcagg cgctgaaggt ccatcgccaa ccagggggcg tggcctgggc gatctgggac
    11461 gagcgctccg acgccatcgc catccagcag gcgagccaca aggaggcgaa agacgcgggc
    11521 gccatcaagc gctgcgacgc cgtcgaggcc atcgcgcgtg tcgtcggctg cgatccgcag
    11581 gcgctggcgc agacgttcgc cgacgtcgca gccgccatcc gcggcgaaaa gcaggacccg
    11641 ttcggccgcg acttcaaagg caagccgccg ctcaagccgc cgtactgcgt ctcgaagatc
    11701 gcgggtgcgc tcacgcacac gcagggcggt ctcgaagtga acacgcagat gcgcgtcctg
    11761 cgcaaggacg gcacgccgct cccgaacctc ttcgccggcg gcggcgccgc gcggggactg
    11821 tccgggccgt cggactgggg ctatctttcc ggttccggcc tgatgaccgc gaccagcatg
    11881 ggccgcctcg ccggcattgc agcagccaaa ctcgtcaaag gataacgatg atcaagtttt
    11941 actacagcac cgcgcccaat ccgatgaaag tcgccctctg cctggaggag atggggctcg
    12001 cctacgaacc gatacctgtg gacacacgca agggtgcgca acatgagccc ggctacctgg
    12061 cgatcaaccc caacgccaag gtgccctcga tcgtggatgg cgacgtgacg atgttcgaca
    12121 gcaacgccat cgtgctctat ctcgccgaga agaccggcaa gttcctgccc aagaaggcgc
    12181 aggagcgcgg gcccatgctg tcgtggctga tgttcgtcgc ctcgggcatc gggcccttca
    12241 ccggccagta cgtgcacttc aagaactacg cgccggagaa gatcgcctac gctctcaacc
    12301 gctacgagtt cgaggcgctg cgccattggc gcatcgtgga tgcgcacctg gcgaagagca
    12361 aatatatggt cggcgatatc tacaccgtcg tcgacatggc gctttggggc tgggcgcggc
    12421 tgttgtccaa tattctgggc gagcaggcgg cgaagtttgc caacgtgaag cgcctggtgg
    12481 acgagatcag cgcccgcccg gcagcgcaga aggcggtggc tctcaaggac cgctacacct
    12541 tcaagaccga gatggacgac gaggccaagc gcgccatgtt cccgggtaac taccgggcgc
    12601 aggctggcgc gtgaagcgcg tttaatcgga ggttctaatg ccacgcatga tcacgttggg
    12661 ggcggcacaa accggcccgg tcaacgaaga cgactggcgt cccaacgtgc cgggcgcctg
    12721 cgccatgatc gaggaggccg gacgccgcaa ggtcgacatc ctgtcgttct cggagctgtt
    12781 cctcacgccc ttctttccca accggctcac ccgggactac gacaagttct tcgtcacgct
    12841 ggacgagccg gagctggcgc cgattttcaa ggccgcggtc gacagccgcg tggcgctcgt
    12901 gtttccgttc ggcgaaaaga acggccgcca ctaccacaac tcctgcctgg tcacggacaa
    12961 gaacggcaag cgcctgggca tttaccgcaa gacgcacatt ccggcgatcc tgccctcgaa
    13021 cctgcccggc ggaacgggaa gctacgagaa gttctatttc tcgccgggca ccgagtttcc
    13081 ggtgttcgat ctttgcggca cgaagattgg catccagatc tgctacgacc gcaaataccc
    13141 ggaaggctcg cgcctgctcg cgctgaaggg cgcggagatc ctgttcatgc cgatctgcgc
    13201 cgcaacctat ggcgagacca agctgcgcgg caacacctgg gagctcccgc tgcaggcgcg
    13261 cgcctacgag aacggggtgt acgtggtggc ggtgaaccgt tgcggaatgg agcaaggccg
    13321 caaccatatc ggccgcagca tgatcgtgga cccgataggc gccgacatgc tggcggtcgc
    13381 ctcctcggac acgcccgagg ttctcgtggc gacggttgac ctggacaacg tcgccgccgc
    13441 gcaaaccagc ctgccctggt ggcgcgaccg ccggccggag ctctacgcgt cgctcgccgc
    13501 ggctgtctag tgagtcacgc gtagcgccgc cgacagcgcc ttcaggtcgc tggcggcgcc
    13561 aaggtcctgc gccgcacgct cgagcgctcc gaccgcatcg ctcttcagcg accgcgcagc
    13621 gttcgcgcgg aacttggcga gcaccgcttc cgagcccggg gggcgggtgt ggttgccgaa
    13681 cacgtcgtcg atccggatcg tctgggtttc ggtcttggtc ttgcagacta cctcggcttc
    13741 gaagcgctga gggaagcgcg ccccgggcag cggctcccag gcgatgcgct gggcgagctg
    13801 ccgcaccgct gcccccgccg gcgcctcgaa cgtcgcgaga tcgacctttc cctccaccag
    13861 ctgcgccgcg acggcgatcg gcaggctcca gcgtgcggcg tgaccgttgt ccggagcctg
    13921 cttgcgctcc cacggctcgc agatgaccgc cgcttcgcct tgcggcacgc ggcagacgat
    13981 gcgctcgacg tcgcccgggc gcacgccgcg ctcggccagc ttgcccgcgg cctcgatgaa
    14041 cggatgcagg tagtggcagc aggggtagaa cttgtacgcc gctttcggca ggtgccattc
    14101 gcggccgatg ccgccgatca aggcgcggaa gcgcccggcg gcggcttcgt cgccggtgaa
    14161 ctggcggaac aggccgtgct tgccgtcgag cgaagtctcc ggtccggtca tccccgagcg
    14221 cgccagcatc gccgccacca cgccgccgtg cgccgcccac cccgggtgga gcgactttac
    14281 cgacgagccg ttggtgagga attccatcac gcccgaggcc tggctgagcg cgatgccgac
    14341 cgcggccacc gtgcgctctt cgtccaggcc catcagctcc gcggcgagca gcgccaccgc
    14401 aagcgtgccg gtgaccgagg tgctcatgaa tcctttcgcg tggaagccgt tcggcgccgc
    14461 gaggccgaag cggatcagca cctcccaacc tctcgcgtaa gcgccgagca gcgcggcgcc
    14521 gctcgcgttt tgcgcctccg caacggcgag ggcgccggcg gcaagcaccg cgctgccgtg
    14581 cgcgatcgag gcggtgtgcg tgtcgtcgaa ctccaggctg tggatcaggc cgccattgat
    14641 cagcgctgcg tcggcggcgc cggcggcggt cgactggccg agcacggtgg cgggtccgcc
    14701 cttgacgacg tcggcggcga agccgcggta cgccgcgccg attggcgatc cggccgatgc
    14761 gaggccgacg ccgattgcgt ccagaaaatg cagccgtgcc gcagtcgcga cgtcttcagg
    14821 caaaggcgtc gaggcggcgg ctcgcgccgc gcgcacgagc tcgcgggcaa gggtcatgcc
    14881 ttgaccatcc gggccagggc gtcgcgccag gattgagaaa gaagctcgga atcgccggcg
    14941 atgaccttct tcatcgtctc ggggatcgcg gggaacaccg agccggcgtt ctccgccagc
    15001 ttggagagca gcgtcggctc gtcgaagggc ttgtcggcgc cgccgcgcgc gctttccacc
    15061 acggcgctca tctcggcgcc gtcgccgaag cgcagcgtga cgcgcgccgg ccggtcgttc
    15121 ggtgcgggct tgatgtcggg atagggctgc aggcgcacgc gcgtgcgcag cttggagatc
    15181 tccgcgtcct cgagcttgtc ctgcgtgaag gcgcgcgctc ccgcggtgcc gagctgtacc
    15241 gtcgcggcga tggcgtgggg cagcgaaaac ttggccgcga gcacggtctc gggctcgacc
    15301 gtgcgcagcg cctggccgcc cggcgccgtc tccaccacga tctcggcgat ctcctccttt
    15361 ttcctctgca tgcggccgag aagctcgagc gtcgcttcga cggcggagtg cgcgtagccg
    15421 cagcaggcaa agaccttgtg gtagccgttg gtgaccgacc acgtgtcgcc caggcccgcg
    15481 acgagggcgt ctggtttcgc atttgtcttg taggcgccga cgaaaacatc gtagggcgta
    15541 acgtcgttgc cgccgatccc ggcctcggcc cagtcggcgg cgcgcagccc gagccacgcg
    15601 ccggccgagg tccaggcgtt gcgtatcagc gcgccctcga ccgcggtgtc gaacggcccc
    15661 gcgaaggaca tgctcgcgcc gccggtcacc gccgcgagca tggtcttcgc atcctgcttg
    15721 cgggcgagcg ccgccgcggc ggcaccgccg atcgcggcga acgcggcgtg cggatgcacg
    15781 ttgaacaccg ggaagggaaa cgcgagcgcg aagcgcgtgg tgacttcgta tgcgatggcg
    15841 agcgcgcgca gcacttcctc gaccgtgcgc ccttgcgcct cggactcggc gagcagcgcc
    15901 gggatggtgt acgcgccgcc gtggcaggac gcgttgcgga acccttcgtc gagctcgcac
    15961 caggtgatgg ccatgccgtt ggcgctggcg gcggcgtagc ggtcggcgcg aggcgcgccc
    16021 ttggcgagca ccgtggcttc cgatttgccg gaggaggtgc gcagcagaac ttcgcgcgcc
    16081 ttggcaacct gcgtctcgag cgatccggcg accatcgcgc cgatgtcgtc ggagaggatg
    16141 atcgccgcgc gccggcgcac cggctcgggc agcggcttgc ggaccgcctc gacggcccat
    16201 tcgatcagct tgcgcgtgtt ggcggcggct tgcgcgcgcg ccggattttc attgggtgtc
    16261 atatcgaaga atcctgttca caagttaagc tgtcggtgtt gcctaagggg aaaactcatg
    16321 cgcatctggc atcagagctt taccgatctg acggtcatgc cgctctaccg caagacgatc
    16381 accgagcatg ccgccgcggt gatggggcag gacgccgccg tgagcgtgca cggcctgcgg
    16441 cccggcacgt acaccgaggg gtgcgcgccg atcgacgcga tcaagtatcg ctacgtggcg
    16501 accgtgcacg agctccaggt ctgcgacgcg gcgctcgtgg cgcagcgcga gggcttcgac
    16561 gcgttcgcgc tcggctgctt cttcgacccg ggcctgcggg cggcgcgctc gctcgtcgac
    16621 atccccgtgg tgggtctcgg agagacatgc gcgctcgtcg cctgctcgct cgcccggaaa
    16681 ttcggcctgg tcacgctgtg cgaggaccaa tccgccgact acaccgatct catgctggcc
    16741 tatgggctgg agcgccgctt cgccggcgcg ctcgcgctcg acccgccgat cgacgagttc
    16801 gcgctcgagg ccgacgagaa ggccgcgcgc gcgatcgagg agcgcttcga tgtcgcgtgc
    16861 gcggcgatgg tcgcgcgcgg cgccgagatc atcatcccgg ccgacggggt gctcaacgag
    16921 ttcctcgtgc gccgccgccg cttgaccgcg cgcgggggag tgcccgtcat ggacagcctc
    16981 gggacgctgt tccagcacgc cgccttcctg gtaaggctcg cgcgcacgac ggggaccggg
    17041 atcagccgtc atcagctctt tgccaagccg tcgcgggcga tggtggagca cgtgcggggc
    17101 ttctcgcggc agcccgagct caagagcgac ttctcgggag atcgctgacc gatcaccgag
    17161 tcttccccgg aatcggcgcc tgcccgatgc ggcgccagag atacttgccg tgtccgcctt
    17221 tgccgacgat cttgtggttg tccattacgg tctcgccgcg cgagatgctg cgcaccggcc
    17281 agcccttcag gttccacccc tcgtgcaggt tgtagtcggc gtaggaggcg aagtcggctg
    17341 cgtgcagcac acgctcctta ttcaggtcga cgagcgtgaa gtcggcgtcc gcgccagggc
    17401 tcagtgagcc cttgagcggc gcaaggtcga acaccttcgc cggaaccgag ccgaggagct
    17461 ggcagatgcg ctggagcggg agctttcgct tgtggtagcc ctcggaaagc atcaccgaaa
    17521 gcatgctcgc ggtcgcaggg aagccctgcg acgccagcca aagcggcttg tccttgttca
    17581 acttcttgcg cgcgttgtgg tcggatgcga tgagatcgac ggtgccgtcg aagatcgctt
    17641 cccacagcgc ctcgcggtcg tccttggtgc ggaacggtgg attggccttg cccatgccgc
    17701 cgaggtccga gtcctcggtg tgcgtgaggt agtgcgggca ggtctcgacg atgacatcct
    17761 ggaagcgctg gcgccagtgg cggatcacgt tgagcgcctt gcgcgaggag atgtggggaa
    17821 agtagacgcg cgcgccgagc tcctcggcca ggtacatcgc gcgcagcgcg ggctcggatt
    17881 cggtgatcgc gggcttggaa gcggaccagt ccttgagcgt ggtgccgccc gccgcctgga
    17941 tcttgcgcct ctcgcggttc acgatctcga tattctctgt atggcagacg acgatcgggc
    18001 gcccgacgcg cgcggattcc ttgagcaggt cgtagaagaa gccgtcgtcg gtgccgtcca
    18061 gaccgaggta gcgtccttcc tcgcccttga agttcatgaa gtacttgaac gaggtgacgc
    18121 cgtagtcgcg gacgtactcg ccgagctcct tgatatgcag ctcgttggcg gtgctgaaat
    18181 ggaagccgaa gtccacatgg gcgcgcgcga tggcgtgctc gagctcgcgc cggaagacgt
    18241 cgccataggc ctcgttgttg aggaagtagc caagcaccgt ggtgaagccg ccctgcgcgg
    18301 cgttggcggt ctcggtcgag tactcggtga tcttctcgcc catgccgaag tgcacatggc
    18361 agtcgatcag ccccggaaag acgtgcaggc ccgcggccga ttgctcggcg acgccctggg
    18421 tgggcgcgcc aggcgcgagc agcttctcga tctttccacc cttgacgagc acgtcctgct
    18481 tctccggctc ccggtccggg aacaccagcg tgccgccgcg caatacgaaa tcgctcattg
    18541 ctgtgcctgc ctctcttgga gatccttcag tgaatagacg gcctgcgtac cggccggcgt
    18601 gttgagcgcg ggcagggtcg ccgcgatgtc ctgctcattc aggtcgcgcg cgatgcagac
    18661 caggcgattg gcctgatcat cgtcgggcca accgtcgaag cgctgcggcg cgtggaacac
    18721 gccctgcacg ccctggacga aggcgatttc gccggtgtcc tccatcttca gaaggccttt
    18781 cacgcgaatc aggcgcgcgc cgaactccgc ggccaccagc cgcgaccagg ccgaccagcc
    18841 ggcccaggtg acgggcgagg gcgggcggaa ggcgtaggcg cggatccccg agaaatgctc
    18901 gcggttgcgg atcgggccga gcgtcgcgat cgatgcgacg ggttttcgcg ctttcccgtg
    18961 ggtggaaaag attcttgaaa tggcatccac cagctcggca tggggattca tttccttgag
    19021 aaaggcaatg accggtccgg gatcggccag gtctttcttg gtgacgacaa tgcgctctgc
    19081 cattgcgacc tgcttgtcca gttcggggta ctgcaggcgc gagga
//
LOCUS       DATBSZ010000080         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_216793, whole genome shotgun sequence.
ACCESSION   DATBSZ010000080 DATBSZ010000000
VERSION     DATBSZ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216793"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VIG70_05380"
     CDS             <1..226
                     /locus_tag="VIG70_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250020.1"
                     /translation="EITVGQNNTWAIPALEFRGVPVGIDVRRVVETGIQPAINTGIAH
                     RRAGVGQVGAGVARAPLACFEKALRALGRP"
     gene            207..1577
                     /gene="fdrA"
                     /locus_tag="VIG70_05385"
     CDS             207..1577
                     /gene="fdrA"
                     /locus_tag="VIG70_05385"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HEY8250021.1"
                     /translation="MPSVVLNRVERGRYLDSVAAMRLSRRLEALPGVEGAALMIGSPS
                     NKTLLRDAGLLAGDGEAAGANDLVIAVRASDEGSGRRALDSALNLEPEKTAGLAHART
                     LGGAIERLPQANLALISVPGDFAALEARKALDAGLHALVFSSGVSLEEERALKLVAKE
                     KGLLLMGPDCGTALIAGTPIAFANAVPRGDIGIVSASGTGLQEVSCLIAHLGGGVSHG
                     IGVGGRDLHEQVGALGTLAAIDALEADPATKTIVLISKPPAPQVAEEVLARLSRSRKR
                     SVVCFLGLDKPGLARTLREAAELATGKKIPDERPEKRKIKGRVEALYCGGTLCSEAEL
                     VFRRLGLAGHRFVDLGDERHTRGRPHPMIDPEIRNDHIARALADPEVGVILVDLVLGY
                     GSHPDPAGVLIKQEIKKTFVASVTGTETDPQVRSRQVAKLREAGVLVAPSNAHAAEWA
                     ASFVSN"
     gene            1650..>2091
                     /locus_tag="VIG70_05390"
     CDS             1650..>2091
                     /locus_tag="VIG70_05390"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HEY8250022.1"
                     /translation="MTPGQSYAVSVTMQNTGGKTWTAAINHRLGSQNPQDNTTWGGNR
                     VNLPSSTVTGANATFSFNVTAPTTPGTYNFQWKMVQDLVEWFGAQSANATIKVGLDNA
                     AFVSQSVPTTMLAGQGYPVSVTMQNTGSTTWAASTVGIGSQNPAD"
BASE COUNT          354 a          741 c          693 g          303 t
ORIGIN      
        1 cgagattacg gtcggccaga acaacacatg ggccattccg gcgctggagt ttcgcggcgt
       61 gccggtcggc atcgacgtgc gccgcgtggt cgagaccggc atccagccgg cgatcaacac
      121 cggcatcgcg caccgccgcg ccggcgtcgg ccaggtcggc gccggcgtcg cgcgcgcgcc
      181 gctcgcctgc ttcgagaaag cccttcgtgc cctcggtcgt ccttaaccgc gtagagcgcg
      241 gccgctacct cgactcggtc gcggcgatgc gcctttcgcg gcgcctcgaa gcgcttcccg
      301 gcgtcgaagg cgcggcgctg atgatcggca gcccgtcgaa caagaccctg ctgcgcgacg
      361 ccggcctgct ggcgggagac ggcgaggcgg cgggcgccaa cgatctcgtg atcgccgtgc
      421 gggcctccga cgaaggatcc ggccgccgcg cgctcgattc cgccttgaac ctggagccgg
      481 agaaaaccgc aggccttgcg cacgcccgca ccctcggcgg cgcgatcgag cgcctgccgc
      541 aggcaaatct cgcgctcatc tcggtgccgg gcgatttcgc cgcgctcgag gcgcgcaagg
      601 cgctcgacgc cggcctgcac gccctcgtgt tctcgtcggg cgtgtcgctc gaggaagagc
      661 gcgcgctcaa gctcgtggcg aaggaaaaag gattgctgct gatgggcccg gactgcggta
      721 cggcgctcat cgcgggaacg ccgatcgcct tcgcaaacgc cgtgccgcgc ggcgacatcg
      781 gcatcgtttc ggcatccggc accggcctgc aggaagtgtc gtgcctgatc gcccacttgg
      841 gcgggggcgt gtcgcacggc atcggcgtcg gcggccgcga cctccacgag caagtgggcg
      901 cgctcggcac gctcgccgcc atcgacgcgc tcgaagccga ccccgccacc aagaccatcg
      961 tcctcatttc caagccgcct gcgccgcaag tcgccgagga ggttctcgcc cgcctttcga
     1021 gatcccggaa acgcagcgtg gtgtgcttcc tcgggctgga caagccgggc ctggcgcgca
     1081 cattacggga ggccgctgag ctcgcgaccg gaaagaaaat tccggacgag agacccgaga
     1141 aaagaaaaat caaaggccgt gttgaagccc tctactgcgg cggcacgctc tgcagcgagg
     1201 ccgagctcgt ctttcgccgc ctcggcctcg ccggccaccg cttcgtcgac ctgggcgacg
     1261 agcgccacac gcgcggccgg ccgcacccga tgatcgatcc cgagatccgc aacgatcaca
     1321 tcgcgcgcgc gctcgccgat cccgaggtcg gcgtgatcct ggtcgacctc gtgctcggct
     1381 atggctcgca tcccgacccg gccggggttt tgatcaaaca ggaaatcaag aagacattcg
     1441 tcgcctcggt caccggcacg gaaaccgatc cgcaggtgcg ctcgcggcag gtggcgaagc
     1501 tccgcgaggc gggcgtgctg gtcgcgccgt ccaacgcgca tgccgccgaa tgggcggctt
     1561 ctttcgtctc gaactgagac tcataggtag gtcgtcgtaa aggaagggcc ggcgctcgcc
     1621 ggcgttttcg tgctgcactc gcgccggtca tgaccccggg ccagagctat gcggtcagcg
     1681 tcacgatgca gaacaccggc ggcaagacct ggaccgccgc catcaaccat cgcctgggct
     1741 cgcagaaccc gcaagacaac accacctggg gcgggaaccg cgtgaacctg cctagctcga
     1801 ccgtcaccgg cgcgaacgcg accttcagct tcaatgtgac ggcgcccacc acgcccggca
     1861 cgtacaactt ccagtggaag atggtgcagg acctagtcga gtggttcggc gcgcagtcgg
     1921 cgaacgccac gatcaaggtg ggcctggaca acgcagcgtt cgtctcgcag agcgtcccca
     1981 cgaccatgct ggcgggacag ggctatccgg tgagcgtcac catgcagaac accggcagca
     2041 ccacctgggc ggcaagcacc gtcggcatcg gctcgcaaaa tcccgcggac a
//
LOCUS       DATBSZ010000081         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1446843, whole genome shotgun sequence.
ACCESSION   DATBSZ010000081 DATBSZ010000000
VERSION     DATBSZ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446843"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VIG70_05395"
     CDS             <1..455
                     /locus_tag="VIG70_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250023.1"
                     /translation="AYVAEVVWGTEEDVSFRARGWPISQPAQNNIKPKWFGGSVMGHW
                     ELSRASALTLRYDYLDDQDGWRTGVDQKLNAATVVYTLHLSALAGKAPLAVTYPRTQV
                     RIHDVDLKMEYRYNWSNVQVFSDAAPPFADTDPKKAGHQIQIQAAINF"
     gene            466..1038
                     /locus_tag="VIG70_05400"
     CDS             466..1038
                     /locus_tag="VIG70_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250024.1"
                     /translation="MIARSLIMMLALAASTAAAHITPPVVLVPERDALASQLAGAKKL
                     SLREVSLTADERKAVQSTYGWRAGDELHAFYMGRDDAGKLLSATVFMTQATMHGVIRV
                     AVGLTPDGRVKVAEVVEVSEEIYGHMKPLIDRGFMKRHAGLWIGSSFEPPAAGGGADA
                     MAQHYDAIVSRLVQRAVILYDVCVTKRGVT"
     gene            1042..2556
                     /locus_tag="VIG70_05405"
     CDS             1042..2556
                     /locus_tag="VIG70_05405"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY8250025.1"
                     /translation="MRAARWLLALAAAWAGGAAGHAGLVSSTPADGAALAAPPASIEL
                     RFTEPVTPMVMRLFAGSAEPVTLAAPQGASDVIKLALPANLREGLHTLSFRVISADSH
                     PIGGSIVFAVGERPPPPRAATSDAGGPSIGMTLARALRDLALLVAAGGALFALAIARF
                     PAERQVLGIAGLLAAVAALACIGVHGAAMTDAAVTSREAWRSGLHSSFGLSACLAAAG
                     AVCIAAGAFAASAVRAGLLLAGALAVIASLPLTGHAMTAKPTAVAVAALAAHGLAAAF
                     WIGSLVALLSIMNVAVLRRFSRWAMVAVALLVIAGVAFAVLQVRSVEELFGSDYGWLI
                     VGKVFFLTLLLVLAVLNRFRWLPMLERGARAAAARLRWSIAGELALAVCVIAVTAVLV
                     QTPPPHGFTQKVAHKEDVAEVSVTPARAGTNAIVVRFQDKDGQPFDPEEVLLEIGNEA
                     AGVEPAARAIRRVGPGHYSREGSELAFPGVWTIEVHARLDASGMASFRSQVPIR"
BASE COUNT          362 a          882 c          901 g          417 t
ORIGIN      
        1 ccgcgtacgt cgccgaagtg gtatggggta cggaagagga cgtctccttc cgcgcgcgcg
       61 gctggccgat ctcgcagccc gcgcagaaca acatcaagcc caagtggttc ggcggctcgg
      121 tcatggggca ctgggagctg agccgtgcgt cggccctcac gctgcgctat gactatctgg
      181 acgaccagga tggctggcgc acgggcgtcg atcagaagct gaacgcagcg accgtcgtct
      241 acacgctgca cctgtcggcg ctggcgggga aagcgccgct cgccgtgacc tacccgcgca
      301 cgcaggtccg tatccacgac gtcgacctga agatggagta ccgctacaac tggtcgaacg
      361 tgcaggtgtt ctcggacgcc gcgccgccct tcgcggacac ggatccgaaa aaagcgggcc
      421 accagatcca gatccaggcg gcgatcaact tctaaggacc cggccatgat cgcgcgcagt
      481 ctcatcatga tgctcgcact ggcggccagc actgccgccg cccatatcac accgccggtg
      541 gtgctggtcc ccgagcgcga cgcgctggca tcgcagctcg cgggggcgaa gaagctgtcg
      601 ctgcgggaag tcagcctcac tgccgacgaa cgcaaggcag tgcagtcgac gtacggctgg
      661 agggccggcg acgagctgca tgccttctac atgggacgcg acgatgccgg aaagctgctt
      721 agcgcgacgg tcttcatgac ccaggccacg atgcacggcg tcattcgcgt agcggtcggc
      781 ctgacgcccg acggccgcgt gaaggtcgcg gaagtggtgg aggtctccga ggaaatctat
      841 ggccacatga agccgctcat cgaccgcggc ttcatgaagc gtcatgcggg cctgtggatc
      901 ggctcgtctt tcgagccgcc cgcagccggc ggcggcgccg acgcgatggc gcagcactac
      961 gacgccatcg tgagccggct ggtgcagcgc gcggtgatcc tctacgacgt ctgcgtgacg
     1021 aagcgaggcg tcacctaggg gatgcgcgcg gcgcgctggc tgctcgcgct tgccgccgcc
     1081 tgggccggcg gagcggccgg acacgccggg ctggtctcat cgaccccggc cgacggcgcc
     1141 gcgctcgccg cgccccccgc gagtatcgag ctgcgcttca ccgagccggt gacaccgatg
     1201 gtgatgcgcc tcttcgcagg ttcggcggag ccggtcacgc tggcggcgcc gcagggcgcg
     1261 agcgacgtca tcaagctggc gttgccggcc aacctgcgcg aaggcctgca caccctcagc
     1321 tttcgcgtga tctcggcgga ctctcacccg atcggcggct cgatcgtgtt cgcggtcggc
     1381 gagcgaccgc cgccgccgcg cgccgccacc agcgacgctg gcgggccgtc gatcggcatg
     1441 acgctcgccc gtgccctgag agatctggct ttgctggtcg ccgcgggcgg cgccctgttt
     1501 gcgctcgcca tcgcccgctt tcctgccgag cgccaagtcc tgggcatcgc aggcttgctg
     1561 gccgccgtcg ctgcgctggc ctgcatcggc gtgcatggcg cggcgatgac ggacgcagcc
     1621 gtgacctcgc gcgaagcatg gcgcagcggc ttgcacagct ccttcggcct gagcgcctgc
     1681 ctcgccgccg cgggggcggt gtgcattgcc gcgggagcat tcgccgcgag cgcagtacgg
     1741 gcgggtctat tgctggccgg agcgctcgcc gtgatcgcga gcctgccgct cacgggtcac
     1801 gcgatgaccg caaagccgac cgcggtggcg gtggcggcgc tcgccgcgca tggcctcgcc
     1861 gcggcgtttt ggattggatc gcttgtcgca ttgctctcaa tcatgaacgt cgctgtcctg
     1921 cggcgtttct cgcgctgggc catggttgcc gtcgcgctcc tcgtgatcgc gggcgtcgcg
     1981 ttcgcggtgc tgcaggtccg ctccgtcgaa gagctgttcg ggtctgacta cggctggctc
     2041 atcgtgggga aggtgttctt cctcaccctg ctgctcgtgc tggcggtcct caaccgcttt
     2101 cgctggcttc cgatgctcga gcgcggcgct cgcgccgcgg ccgcgcgcct gcgctggtcg
     2161 atcgccggcg agctcgcgct ggcggtttgc gtgatcgccg tcaccgcggt gctggtgcag
     2221 acgccgccgc cgcacggctt cacgcaaaag gtcgcccaca aagaggacgt cgccgaagtt
     2281 tcggtgaccc ccgcgcgcgc cgggacgaac gccatcgtgg tgcgcttcca ggacaaggac
     2341 gggcaaccgt tcgatcccga agaggtgctc ctcgagatcg gcaacgaggc ggcaggtgtc
     2401 gagcccgcgg cgcgcgcgat tcgccgcgtc ggtccgggcc actacagccg cgagggcagc
     2461 gagctcgcct ttccgggcgt gtggaccatc gaagtgcatg cgcgcctcga cgcgagcggc
     2521 atggcgagct tccgctcgca ggtgccgatt cgctagcggc ga
//
LOCUS       DATBSZ010000082        21914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1446935, whole genome shotgun sequence.
ACCESSION   DATBSZ010000082 DATBSZ010000000
VERSION     DATBSZ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21914
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446935"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VIG70_05410"
     CDS             complement(<1..235)
                     /locus_tag="VIG70_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY8250026.1"
                     /translation="MDGKQSTVAPKQLLTVWDGIILICGMVIGAGIFKAPSIVAGNTS
                     SGAEFLGAWVLGGAISLCGALVYAELATRHPETG"
     gene            complement(211..1038)
                     /locus_tag="VIG70_05415"
     CDS             complement(211..1038)
                     /locus_tag="VIG70_05415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8250027.1"
                     /translation="MAVLPAAAQPQAWAWDDGTVPYVQTPMEIVERMLRMAEVGRDDY
                     VIDLGSGDGRIIIEAAKRGARGLGVDLDPSLVKHATENAQRAGVGDRTKFLVKDIFET
                     DLSGATVVAFYLLPDFNAKLLPQLLKLKPGTRIVSHDGGIGDWPPDERLEMRAPEKPV
                     GVGGVSRVELWIVPANAKGAWTSELPGHGGTWKFQVKQHFQVLDIDVAAQGRDLLVRN
                     SRLRGEEIKVIVTGVVNGRAWHHYFVGTLRDDRITGEVTVSDGNNKRSFPWTASRAR"
     gene            1113..1188
                     /locus_tag="VIG70_05420"
     tRNA            1113..1188
                     /locus_tag="VIG70_05420"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1146..1148,aa:Arg,seq:cct)
     gene            1388..1810
                     /locus_tag="VIG70_05425"
     CDS             1388..1810
                     /locus_tag="VIG70_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250028.1"
                     /translation="MKLSKAMILGLALAAGGCSMLEVQPIAKETLKNSQGHVIGYRER
                     LFDGAIGEELDRVVIFTPRHSERGAVVAYEERIKGGVLLHDLKGKRIGSRYVDLRSRG
                     SNARNGGLTIVFVPREADRLAVAQVTIEEIKQHLNITN"
     gene            complement(1824..3344)
                     /locus_tag="VIG70_05430"
     CDS             complement(1824..3344)
                     /locus_tag="VIG70_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY8250029.1"
                     /translation="MFELLGAGIVNLMQVKYLLPLFLGTLAGVLGGALPGVTITMTII
                     VVLPFTFGLDPLQGLAAMTGVYVGGSAGGLVTATLLGLPGTPSAIATTFDGHPMAKKG
                     EPGRAIWLGIWASFFGGLLGGVFLIGATEPLAAFALRFGPWEFFALFILALSMVAGLA
                     EASLLKGLLSGCLGLIVTILGTDPVMGQDRLTLGIPFIKGGIDFLPVLIGVFAFSQVM
                     GEIERGGAPAADAVERAGRLVVSQARVIWEILSRPFQLLWFAFIGVVIGVLPAIGGSA
                     ASMMAYDQAKKISRRPQEFGSGVPEGIIASESSNNANVGGSLVTIMAFGIPGDAVTAV
                     MLGALTIHGIQSGPLFISQNTQLAYGIFAAYLLAHPLMVLIMAAGARWMLRIVTVPKA
                     ALFPVVLVLCTIGAYALNNTMANVYVLLAFGVLGYAMVKVGLPLAPFILGVILGDQIE
                     INLVRSLMTDANLWLFVTRPISALLLGAAAASIVLSIWQHRRMQKKSAVAAGAADF"
     gene            complement(3347..3811)
                     /locus_tag="VIG70_05435"
     CDS             complement(3347..3811)
                     /locus_tag="VIG70_05435"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY8250030.1"
                     /translation="MKLSKDGWSGLAVLAASLFLFALTLGLKDNPLVPIGPGFYPRIV
                     LGLTALFAVALVAFDLLRKATPPAGPHAAYGAVVLHFAVFAVYAVALPWVGFRIATFV
                     YVAATNALLDVPRSVRGWLRVALLALGTVLATYYMFEHYLSVLLPRGRWTNF"
     gene            complement(3808..4746)
                     /locus_tag="VIG70_05440"
     CDS             complement(3808..4746)
                     /locus_tag="VIG70_05440"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250031.1"
                     /translation="MTRLLAGIALALAPLASFAAYPERPVKMIVPWAAGGDTDNIFRP
                     FGQVMQKHLGQTVVIANVGGASGTRGAKEAKDSPADGYTLYAVHDYIHSTYWAGVADV
                     QYTDFEPICLISSTPSVLTASPKTPWKSWKDLLADAKARPGQITVGATLASTSHFFPA
                     LIEKASGVKFKYVSYEGLAPRMNAILGGHVDLTDSNLTQKGKVEAGQLKFLAIATEKR
                     HPEMPNVPTLKELGVNVVYDVNRGVMTPKGTPADVIAKLASSCAAAAKEPEFAQAMKL
                     QGTDVRYMDRAAYGKWLKQNDDLNRNLAKDLGLLKR"
     gene            complement(4775..5803)
                     /locus_tag="VIG70_05445"
     CDS             complement(4775..5803)
                     /locus_tag="VIG70_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8250032.1"
                     /translation="MKRVLLIGAGHAHLVALRSLAQKPLYGARITLVSPLARQVYSGM
                     LPGILAGHYRRAEAEIDVAALAERGYVEFERGIVERFDAGKRLVALKDGRSSGYDVVS
                     FNVGSLIAASIPGSEHALAVKPFDEFLARLRIAERVAVIGAGAAGAELAMALRHRGAE
                     VTLYSETPVLAPRVLQQLRRRKVDFRPGMPVTAIEPGPVVIAGTARQDFELVLLATGA
                     APMPWLRQSGLQTDERGFVLVGATLQSVSHPEVFALGDCGTLRDSPHPKSGVYAVRHG
                     ESLVDNLRRLVVGAPFEPYVPQEKALSLMSCGARYAIAERAGWSAQGRWVWWWKDGID
                     RRWIASFR"
     gene            complement(5800..7443)
                     /locus_tag="VIG70_05450"
     CDS             complement(5800..7443)
                     /locus_tag="VIG70_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8250033.1"
                     /translation="MPDRYAELYASFRWNVPERYNMARACCGNWAGERHRFALYWEDE
                     SGAMAAYSFWDIQQAANRLSNALVALGVKRGERVAIILPQRPETAIAYMAVFQMGAIA
                     LPLSHLFGPDALEYRMQHAEASIAIVEPATIVNLWAIKDRLSHLRHVIGVGGANETKV
                     RRWEDLLGSASSRFTPLDTKADEPALIIYTSGTTGPPKGALKAHRVLLGNAPGFVHSH
                     DFFPRPGDVFWSPADWAWTGGLMDALLPAWLFGIPILGYRGRFDAEKAYYLLEKYGIR
                     NSFLFPTALKLMMKAVPQPRGKYDLNLRSVMSAGESVGVTVFEWAKEHLGVTINEMFG
                     QTEINYVVGNCQSAWPAKPGSIGRPYPGHRVAVIDDAGKPLPANELGEIAVNRSCNGQ
                     RDPVFFLEYWKNEQATREKFIGDWGCTGDQAKMDEDGYLWYQGRSDDVIKSSGYRIGP
                     AEIESCLVKHPAVLNAAVIGKPDAARGAIVKAFVVLQPNYSPGESLVEDIQNHVRGRL
                     APYEYPREIEFIDALPMTTTGKVQRKELRKREESKLQKA"
     gene            7442..8110
                     /locus_tag="VIG70_05455"
     CDS             7442..8110
                     /locus_tag="VIG70_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEY8250034.1"
                     /translation="MRRFYTSAMAAEAFVFDAYGTLFDVYSVGARCESCWPGKGAQVS
                     LLWRAKQLEYTWQRSLMQRYAPFSTVTREALAYACAALGLELSVAQMEGLMAEYLGLA
                     LYPDVRATLEKLDSRRAILSNGSPDMLQPLVKHSGLRFDAVISVDKLKIFKPAPQVYE
                     LAVKKLNLEKNRIAFVSSNCWDALGAKSYGFRVYWINRAKAPVDRLGFKPDAVLTSLN
                     EVLR"
     gene            8094..8621
                     /locus_tag="VIG70_05460"
     CDS             8094..8621
                     /locus_tag="VIG70_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8250035.1"
                     /translation="MKFCGNCAAPVVKRVPPGDNLPRWVCDQCGEIHYQNPRLVVGSI
                     PEYEGKLLLCRRAIEPRYGYWTLPAGFMENAETTAQAALRETLEEAGAQVELGAPFTM
                     ISVPHVNQVHLFYRARVLGLEFKPGEESLEVALWEEGAIPWKEIAFRTVGLTLKRWFA
                     DRGRGAFGFHAEDLQ"
     gene            8647..9930
                     /locus_tag="VIG70_05465"
     CDS             8647..9930
                     /locus_tag="VIG70_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY8250036.1"
                     /translation="MKILIGLLLAYAPAALAQAPAAAPKIDAANTAWMLTASVLVLFM
                     TLPGLALFYAGLVRTKNVLSVLMQCFGITCVVTLAWVAVGYSIAFGDGNAWWGGLGKS
                     FLSGIQVSTVKGSIPETVFAMYQLTFAIITPALVIGAFAERVRFGGMLLFSVLWLLIV
                     YCPIAHWVWGDGWLMKRGLMDFAGGTVVHLNAGVAALVCALVLGRRRGFPETAMPPHN
                     MTMTVTGAAMLWVGWFGFNAGSALAADGAAGMAMLVTHIGAATGAFAWMLCEWLRYGK
                     PSALGIVTGMVAGLGTITPASGFVGPIGALIIGGAAGVACFFATNYMKRALNVDDSLD
                     VFPVHGVGGLLGTLLTGILVSSAFGGIGYPDKVTMGDQFMTQVIGVVATAVWSGVITW
                     ILLKLVDAIAGMRVATEEETEGLDTVLHNEQGYNL"
     gene            9935..10624
                     /locus_tag="VIG70_05470"
     CDS             9935..10624
                     /locus_tag="VIG70_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY8250037.1"
                     /translation="MERRGFIKFCAASAAALGSPGVAADARAQFYSRARLVDEKGAPL
                     RAAAIPVHRNFIFHYPFAATPCFLLNLGKAVKPAQLKTVAGEPYEWKGGVGAVRSVVA
                     YSAICAHRLSYPTKDISFISFRTEKSARNRIANVIHCCSEHSQYDPAEGARVVAGPAP
                     QPLATILLEHDLKTDELHAVGTLGGEMFGEFFDRFGFKLQLEHGTPRAAVSGSCVVQE
                     LDNYCKQQVKC"
     gene            10618..11319
                     /gene="aat"
                     /locus_tag="VIG70_05475"
     CDS             10618..11319
                     /gene="aat"
                     /locus_tag="VIG70_05475"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY8250038.1"
                     /translation="MLAWLESKDPFPPVERALKNPNGLLAAGGELSVERLLEAYRHGV
                     FPWYSGAEPVLWWSPDPRMVLYCAELKVPRSLAKSVRNKGFEVRIDTAFAAVLEGCAD
                     RPQTWLGADMRKAYLRLHRAGYAHSFETWRKGELVGGLYGVALGRMFYGESMFSQATD
                     ASKVALVALVGELRGRAFPLIDCQMHTPLLASLGARAIPRRSFLRAIATLVNYPEPEG
                     KWTSRAGNRRTLESA"
     gene            11326..12087
                     /locus_tag="VIG70_05480"
     CDS             11326..12087
                     /locus_tag="VIG70_05480"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEY8250039.1"
                     /translation="MSRLNDLPHAVLQFYVTAPYPCSYLPDRMARSQVATPSHLINTE
                     LYGDLVKAGFRRSGIFTYRPHCDACRACVPVRIPVGQFDANRSQRRAWKQHSTLTAHP
                     QPLKFRLEHYALYLRYQSKRHSGGGMDNDSRDQYSHFLLQSRVDTRLIEFRDAGQLVM
                     VSIVDYLPDGLSSVYTFYEPDRRTASFGTYNVLWQIEQCRALALPYLYLGYWIRESPK
                     MAYKSKFEPIEGFVGGTWRRLGRRLTEESKEEKRA"
     gene            12107..13099
                     /locus_tag="VIG70_05485"
     CDS             12107..13099
                     /locus_tag="VIG70_05485"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY8250040.1"
                     /translation="MNLYRLARPLLFSLDPETAHKVGLNLAALAPRRRLHCPVRAMGL
                     EFANPVGLAAGLDKDAAHIDALARMGFGFIEVGTVTPRPQPGNPRPRLFRIPEKEAII
                     NRFGFNNVGVDAFLENVARARWRGVLGINIGKNADTPVERAIDDYELCLQKVYAAASY
                     VTINISSPNTAGLRSLQERDSLEDLLGKLSAARQDLSRRHGKRVPLVLKIAPDLAPED
                     IPSIAASVQRHAIDGLIATNTSLSREGVANLPHAGEAGGLSGAPIRDRSTRVLKEFSA
                     SLPGTTLIGAGGILSGRDAEEKFSSGAALVQLYTGLVYRGPGLVSECVSAFRAK"
     gene            13072..14115
                     /locus_tag="VIG70_05490"
     CDS             13072..14115
                     /locus_tag="VIG70_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY8250041.1"
                     /translation="MRIGIPREVKDGEHRVALSPSGIRGLGDVVVEPGAGQGVGFSDD
                     DYRQAGARLGDPWDCDLVVKVKELQESEYARPRPGQTVFGFQHFGPDPDLLDAALASG
                     ATYLAYETVGQAGGRLPILAPMSAIAGKLAVQVGAWCLLKQNGGSGVLLPGLDGVPPG
                     KVVILGAGNVGSNALAVAYGLGARVAVFAKSEKRFPGLKAKYPHAGFHVGPLGQTLAD
                     ADLVIGGVLTPGEMSPKLIDRKTLRNLRTGSALVDVGIDQGGIAETSRPTSHSEPIYI
                     EEGVVHYCVPNMPAACARTASLALERAVRPFVLKIVKGEIDSDLATAIQVKAGKVTHE
                     HLARDTGRAYSRL"
     gene            14115..14312
                     /locus_tag="VIG70_05495"
                     /pseudo
     CDS             14115..14312
                     /locus_tag="VIG70_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
     gene            14460..15080
                     /locus_tag="VIG70_05500"
     CDS             14460..15080
                     /locus_tag="VIG70_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY8250042.1"
                     /translation="MLGTQDLLLFVLSALLLNITPGPDTLYIVGRSSTQGLKAGVIAA
                     LGISAGIMVHVTAAALGLSAIFTASATAFAVLKLAGAAYLVYVGISLLRTRVVETRAP
                     ERAALRTVFIQGFWTNVLNPKVALFFLAFLPQFVDLDAPGKVIPLLFLGLLFNVNGTL
                     WNLFVAWSSARVGRAVLAHGPAATWFNRSVGALFIYLGVRLALTRE"
     gene            15077..15709
                     /gene="nth"
                     /locus_tag="VIG70_05505"
     CDS             15077..15709
                     /gene="nth"
                     /locus_tag="VIG70_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY8250043.1"
                     /translation="MNPQKRREIFRRLQAANPTPTTELEYRTPYELLVAVVLSAQATD
                     KSVNLATSKLFPATPEKLVQLGVAGVEPYIKSIGLYRTKAKNVVALSRMLLDEHCGAV
                     PQNREALERLPGVGRKTANVVLNTAFGQPTVAVDTHIFRVSNRTGLAPGKDPLEVEEK
                     LLKFTPPEYLKNAHHWLILHGRYVCLARKPGCPQCLIRDLCEFRPKTKDV"
     gene            15702..16127
                     /locus_tag="VIG70_05510"
     CDS             15702..16127
                     /locus_tag="VIG70_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEY8250044.1"
                     /translation="MFNPSREEVRETFFAAWRKYRAGQPLAGIEALALEVILLHPEYH
                     DALASPDRSRDKDYFDESNPFLHMSLHVALEEQLSIDQPHGIAQRFQALVSRFQDRHQ
                     ALHQALECLAETVWRAQRDGAPPDAAAYLDCLAARALRK"
     gene            16234..16845
                     /locus_tag="VIG70_05515"
     CDS             16234..16845
                     /locus_tag="VIG70_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY8250045.1"
                     /translation="MATTERLSLPALFMAACAIAAIAWSSPARTADDSDSIILVAKRQ
                     LQDKLYGSTILIARPIGADRHVGFIINKPTQVTLGKLFPTHEPSQKVADPVFLGGPVS
                     PEVIFALVQGKESPGGRSVKILDDLYLAIDSDVVDRVIEKQPAKARFFAGMVLWRPGE
                     LHEEIKKGLWYVNDARADLVLRKPTESMWEELVGRSERKANAI"
     gene            complement(16850..17176)
                     /locus_tag="VIG70_05520"
     CDS             complement(16850..17176)
                     /locus_tag="VIG70_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8250046.1"
                     /translation="MRGLLVALAAAVFAVPAHAKGNAASGKEKAAQVCAACHGPDGNK
                     PSDPTQPILAGQHYDYLVRALTDYKLGRRNNPIMKGFASQLSKKDIEDLAAWFSSQKS
                     NLHDQR"
     gene            complement(17173..17523)
                     /locus_tag="VIG70_05525"
     CDS             complement(17173..17523)
                     /locus_tag="VIG70_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8250047.1"
                     /translation="MSGAILKIAGFILLGVALAFGAHAQEKKPAAAAKTGQGMENAHA
                     MCIGCHGIPGYKTAFPDVYHVPKIAGQQPGYLVNALKAYKSGERSHPSMRGIAASLSE
                     EDMKQLADYYGGSK"
     gene            17572..18417
                     /locus_tag="VIG70_05530"
     CDS             17572..18417
                     /locus_tag="VIG70_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8250048.1"
                     /translation="MRFKGSQSYVSTEDLTLAVNAAITLQRPLLVKGEPGTGKTMLAL
                     EVARSLGVPILEWHIKSTTKAQQGLYEYDAVSRLRDSQLGDKRVHDIHNYIVKGMLWQ
                     AFTMDQPCVLLIDEVDKADIEFPNDLLRELDRMEFYVYETRELVKAKHRPVVFITSNN
                     EKELPDAFLRRCFFHYIRFPDKETMEKIVAVHFPNLKKSLLREAMEVFFEVREVPGLK
                     KKPSTSELLDWLKLLLAEDIPPEALRSKDRKTIIPPLHGALLKNEQDVHLFERLVFMT
                     RAKGG"
     gene            complement(18426..18836)
                     /locus_tag="VIG70_05535"
     CDS             complement(18426..18836)
                     /locus_tag="VIG70_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8250049.1"
                     /translation="MNGSRQFTPAPKAKVAVIDDDRFIRYLLDLHLRKAGYEVFAAED
                     AVAAGRVIVERTPDVILCDVDMPFMDGYEFVSALRADPATRHIPVIFLTVNKSVGDRA
                     KQLGAGHLVKPVMADELLRAVAQFAPPAQPSAGD"
     gene            18952..21321
                     /locus_tag="VIG70_05540"
     CDS             18952..21321
                     /locus_tag="VIG70_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HEY8250050.1"
                     /translation="MPKDFNLGLVFLSYLVAVTASHVTLLLAARVRDPHATNWKLWVV
                     CGGFAMGIGIWSMHFVATLALKLPIRVLYDLSITALSWVFAIVACGAAFIVLRRLTGK
                     HREFLLPGALIGIGIASMHYTGDASMRLSPGIRYDPALFVASVLIAIGAATAALWIAF
                     HLAKQRAVWTNFGAALVMGAAVVGMHFTAVAAAEFHPASICLASGLRLDEEWMAYTIG
                     GFTFVILTATMLLSAYDARLSSAIAGGAERLRQANEQLEMRVRERTEQLRRAHDAARA
                     GSEAKSAFVATMSHEIRTPLNAVIGMLELFSYSKLSSDQREMLSAARESSRSLLALID
                     DVLDFSKIERGKLEVRPEPCSVARLVDGVNATFEHVASGKGLLLTRRIDPALWPAHLV
                     DPSRLRQILTNLVSNAIKFTHAGTVTLEVKVLAAGEGRQKFCLSVHDSGIGMSADTQG
                     RLFEPFQQGESETSRRYGGTGLGLAISRQLIELMGGTIQVTSTESLGTRVSVTLSAEQ
                     ASVGAIRDDGQTPSGARAPVPVSAPAGTKLLIVDDHPINLAMLKRQLKVLGLEADTAA
                     SGVEALAKWRREGHNLVITDLQMPEMDGYAFARALRADRDQENMPKPTVIAFTANTQS
                     DALERCIDAGMDDYLTKPAELVTLRAKLAQWLGSDAPLRTAPSTRPASAPDGNPINRA
                     RIQQLAGSPQGIAEVLAELESGVRADIAALQVALESANGEALRRAAHRIKGSALTIGA
                     ERLAALAAGVVDAPAGLAEPQLMSSARGLLDELERVLLSARVHRSAPAS"
     gene            complement(21340..21741)
                     /locus_tag="VIG70_05545"
     CDS             complement(21340..21741)
                     /locus_tag="VIG70_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8250051.1"
                     /translation="MANVIDEVTPEVLQAFADAWNRHDVDALMSFMTEDCVFEASAGP
                     DVCGTRYAGHDAVRAGFAEVWATFPDAHWGNARHMVCGERGVSEWTFTGTRRDGTRVE
                     VHGCDLFTFRDGRIALKNSYRKNRPAVGVRK"
BASE COUNT         3691 a         7494 c         7140 g         3589 t
ORIGIN      
        1 cgccggtctc gggatggcgc gtggcgagct cggcatagac cagcgcgccg cagagcgaga
       61 tcgccccgcc cagcacccac gcccccaaga actcggcccc gctcgaggta ttgccggcga
      121 cgatcgacgg cgccttgaag atgccggcgc cgatcaccat gccgcagatc aggatgatgc
      181 cgtcccacac cgtcagcagc tgcttcggtg ctaccgtgct ctgcttgccg tccaagggaa
      241 gctcctcttg ttattgccgt cggaaaccgt gacttcgccg gtgatgcggt cgtccctgag
      301 ggtgccgacg aagtagtgat gccaggcgcg cccgttgacg acgccagtaa cgatcacctt
      361 gatctcttcg ccgcgcagcc ggctgttgcg caccagcagg tccctgccct gcgccgcgac
      421 gtcgatgtcc agcacttgaa agtgttgctt cacctgaaac ttccaagttc ctccgtgtcc
      481 cggcagctcg ctggtccacg cgcccttcgc gttggccggc acgatccaga gctcgacgcg
      541 cgacaccccg ccgacgccga ccggcttctc cggcgcacgc atctccagcc tctcgtcggg
      601 cggccagtcg ccgatgccgc cgtcgtgcga cacgatgcgc gtgccgggct tgagcttcag
      661 gagctgcggc agcagcttgg cgttgaagtc gggcagcagg tagaacgcca cgaccgtcgc
      721 gcccgagagg tcggtctcga agatgtcctt caccaggaat ttcgtccggt cgccgacgcc
      781 cgcccgctgc gcgttctcgg tggcgtgctt cacgaggctg gggtcgagat ccacccccag
      841 gccgcgcgcg ccgcgcttcg ccgcttcgat gatgatgcgg ccgtcgcccg agcctaggtc
      901 gatcacgtag tcgtcgcggc cgacctcggc catgcgcagc atgcgctcga cgatctccat
      961 cggggtctgc acgtacggca ccgtgccgtc gtcccacgcc caagcctgcg gctgcgcggc
     1021 ggcgggaagc acggccatga cccccgcggc tatcagaacc ctaggattca tacagagcgg
     1081 ctaagtctaa ccggactaaa attgcgggcc acgccccggt agctcagtgg atagagcagt
     1141 cccctcctaa gggacaggtc gcacgttcga ttcgtgtccg gggtgccacg ccgctcgtcg
     1201 ccggccgccc accgtccagc catcagatgt ctaaaatttc ataaatataa acaaagacat
     1261 gcatatcgta gtggtgaatt tcgcagcccg cttagggcat tcgtcccttt gcctcgttgt
     1321 ccgaccgggc gtagagtcgc gaccaataaa agcagtgaac aagagggggt gggaaaggaa
     1381 gcacagcatg aaactctcca aagcaatgat tctcggcctc gcgcttgcgg ccggcggctg
     1441 ctcgatgctc gaggtccagc cgatcgccaa agaaaccctg aagaactccc aaggccacgt
     1501 catcggctac cgcgaacggc tgttcgacgg cgccatcggc gaggagctcg accgggtagt
     1561 gatcttcacc ccgcggcaca gcgagcgcgg ggcagtggtg gcctacgaag agcgaatcaa
     1621 gggcggcgtc ctgctgcacg acctgaaggg caagcgcatc ggctcgcgct acgtcgacct
     1681 gaggagccgc ggcagcaacg cccgcaacgg cggcctcacc atcgtgttcg tgccgcgcga
     1741 agccgaccgc ctcgcggtgg cgcaggtcac gatcgaagag atcaagcagc acctgaacat
     1801 cacgaactag agcgttttcg cgactagaaa tccgcggcgc cggcggcgac cgcggatttt
     1861 ttttgcattc ggcggtgctg ccagatcgac agcacgatcg aagccgccgc cgccccgagc
     1921 agcagcgccg agatcggccg cgtcacgaac agccacaggt tggcatcggt catcagcgag
     1981 cgcaccaggt tgatctcgat ctggtcgcca aggatcacgc cgaggatgaa cggcgccagc
     2041 ggcaggccca ccttgaccat ggcatagccg agcaccccga aggcgagcag cacgtagacg
     2101 ttcgccatcg tgttgttgag cgcataggcg ccaatcgtgc acagcaccag caccaccggg
     2161 aacagggctg ccttcggcac cgtcacgatg cgcagcatcc agcgcgcgcc ggccgccatg
     2221 atcagcacca tcagcggatg cgccagcaga taggcggcga agatcccgta cgcgagctgg
     2281 gtattctgcg agataaacag cgggcccgac tggatgccgt ggatggtcag ggcaccgagc
     2341 atcaccgcgg tgaccgcgtc gccgggaatg ccgaacgcca tgatggtcac cagcgagccg
     2401 ccgacattgg cgttgttcga ggactcggag gcgatgatgc cctccggcac gccgctgcca
     2461 aattcctgcg gccggcgcga gatcttcttc gcctggtcgt aggccatcat gctcgcggcg
     2521 ctaccgccga tcgcgggcag cacgccgatc accacgccga tgaacgcgaa ccacaacagc
     2581 tgaaacggcc gcgacaggat ctcccagatc acccgcgcct gcgacaccac cagccggccg
     2641 gcgcgctcca cggcgtccgc cgcaggcgcg ccgccacgct cgatctcgcc cattacctgg
     2701 gagaaggcga acacgccgat cagcaccggc aggaaatcga tgccgccctt gatgaacggg
     2761 atgccgagcg tcagccggtc ctggcccatc accggatcgg tgccgagaat cgtgacgatc
     2821 aggccgaggc agcccgacag cagtcccttg agcagcgagg cttcggccag ccccgcgacc
     2881 atggaaagcg cgaggatgaa cagcgcgaag aactcccacg gcccgaagcg cagcgcgaac
     2941 gccgccagcg gctcggtggc gccgatcagg aacacgccgc ccagcagccc gccgaagaac
     3001 gaggcccaga tgccgagcca gatcgcgcgc ccgggctcgc cctttttcgc catcgggtgg
     3061 ccgtcgaaag tggtggcgat cgccgacggc gtccccggca ggccgagcag cgtcgccgtg
     3121 accaggccgc cggccgagcc gccgacatac acgccggtca tggcggccag cccttgcagc
     3181 ggatcgaggc cgaaggtgaa cggcagcacc acgatgatcg tcatcgtgat ggtgacgccc
     3241 ggcagcgcac cgcccagcac gcccgcgagc gtgccgagga acagcggcag caggtacttg
     3301 acctgcatca ggttgacgat tcccgcgccg agcagctcga acatcgctag aaattggtcc
     3361 agcgcccgcg gggcagcagc accgagaggt aatgctcgaa catgtagtag gtggcgagca
     3421 ccgtgccgag cgccagcagc gcaacccgca gccagccgcg gaccgagcgc ggcacgtcga
     3481 gcagcgcatt ggtcgctgcg acgtagacga aggtggcgat gcgaaacccg acccagggaa
     3541 gcgccaccgc gtacacggcg aacaccgcga aatgcagcac caccgcgccg tacgccgcgt
     3601 gcggcccggc cggcggcgtg gctttgcgga gcagatcgaa cgccaccagc gccaccgcga
     3661 acagcgccgt caatcccagc acgatgcgcg gatagaagcc cggcccgatc gggaccagcg
     3721 ggttgtcctt gaggccgagg gtgagcgcga aaagaaaaag gctcgccgca agtacggcga
     3781 gcccgctcca gccgtccttc gacaatttca acgcttcagc aagccgagat cttttgcaag
     3841 gttccggttc aggtcgtcgt tctgcttgag ccacttcccg taggcggcgc ggtccatgta
     3901 gcgcacgtcg gtgccctgga gcttcatcgc ctgcgcgaac tcgggctcct tcgccgcggc
     3961 ggcgcacgac gaggcgagct tcgcgatcac atcggccggc gtgcccttcg gcgtcatgac
     4021 gccgcggttc acgtcgtaca ccacgttcac gcccagctcc ttcagcgtcg gcacgttcgg
     4081 catctcaggg tggcgcttct cggtcgcgat ggcgaggaac ttgagctgcc cggcttcgac
     4141 cttgcccttc tgcgtgagat tggagtcggt caggtcgaca tggccgccga ggatcgcgtt
     4201 catgcgcggc gcgaggcctt cgtaggagac gtacttgaac ttgacgccgc tcgccttctc
     4261 gatcagcgcc gggaagaagt ggctggtcga ggcgagcgtc gcgcccacgg tgatctgccc
     4321 ggggcgcgcc ttggcgtcgg cgagcaggtc cttccagctc ttccacgggg tcttcgggct
     4381 cgcggtgagc accgaaggcg tggacgagat caggcagatc ggctcgaaat cggtgtactg
     4441 cacatcggcg acgccggccc agtaggtcga atggatgtag tcgtgcaccg cgtaaagcgt
     4501 gtacccgtcc gcaggcgagt ccttcgcctc cttcgcgccg cgcgtgccgg aagcgccgcc
     4561 gacgttcgcg atcacgaccg tttgcccgag gtgcttctgc atcacctggc cgaacggccg
     4621 gaagatgttg tcggtgtcgc cgcccgccgc ccagggcacg atcatcttga ccgggcgctc
     4681 ggggtaggca gcgaaagatg cgagcggcgc gagcgccaag gcgattccgg cgagaagtct
     4741 ggtcatgtca ccctcctcca aaggatgaac gatcttacct gaagctcgcg atccaacggc
     4801 ggtcgatgcc gtccttccac caccacaccc agcggccctg cgcgctccag ccggcgcgct
     4861 cggcaatcgc atagcgcgcg ccgcagctca tcagcgagag cgccttttcc tgcggcacgt
     4921 agggctcgaa cggcgcgccg accacgaggc ggcgtaggtt gtcgaccagc gattcgccgt
     4981 ggcgcacggc gtacacgccc gacttcgggt gcggcgaatc gcgcagcgtg ccgcagtcgc
     5041 ccagcgcgaa gacctcggga tgggacacgc tctgcagcgt ggcgccgacc aggacgaagc
     5101 cgcgctcgtc ggtctggagg cccgactggc gcagccacgg catcggcgcc gcgccggtcg
     5161 cgagcagcac gagctcgaag tcctgccgcg cggttcctgc gatcaccacc ggccccggct
     5221 cgatcgccgt caccggcatg cccggccgga aatccacttt tctccggcgc agctgctgca
     5281 gcacgcgcgg ggcgagcacc ggcgtttccg agtacagcgt cacctccgcc ccgcggtggc
     5341 gcagcgccat cgcgagctcg gcgccggccg cgccggcgcc gatcaccgcg acgcgctcgg
     5401 cgatgcgcag tctcgcaagg aactcgtcga acggctttac cgcgagcgca tgctccgatc
     5461 cggggatcga ggctgcgatg agcgagccga cattgaaaga gacgacgtcg tagcccgagc
     5521 tgcgcccgtc cttgagcgcc acgagcctct tgcccgcgtc gaagcgctcg acgatgcccc
     5581 gctcgaactc cacatagccg cgttcggcga gcgccgccac gtcgatctcg gcctcggcgc
     5641 ggcggtagtg cccggcgagg atgccgggca gcatcccgga gtagacctgt cttgcgagcg
     5701 ggctcaccag ggtgatgcgc gccccgtaca gcggcttctg tgccaggctg cgcagggcta
     5761 cgaggtgcgc gtgccccgcg ccgatgagca ggacgcgctt cacgccttct gcagcttaga
     5821 ttcttctcgt ttgcgcaatt ccttgcgctg gaccttgccg gtcgtggtca tcggcagcgc
     5881 atcgatgaac tcgatctcgc gcggatactc gtagggcgca agccggccgc gcacgtggtt
     5941 ctggatgtcc tccaccaggc tctctccggg agagtagttc ggctgcagga ccacgaacgc
     6001 cttgacgatc gcgccgcgcg cagcgtcggg cttgccgatc accgcggcgt tgagcaccgc
     6061 cgggtgcttc accaggcagc tctcgatctc ggccggcccg atgcggtagc ccgagctctt
     6121 gatcacgtcg tcggagcggc cctggtacca aaggtagccg tcctcgtcca tcttcgcctg
     6181 gtcgcccgtg cagccccaat cgccgatgaa cttctcgcgc gtcgcctgct cgttcttcca
     6241 gtactcgagg aagaacaccg gatcgcgctg gccgttgcac gagcggttga ccgcgatctc
     6301 cccaagctcg ttcgcaggaa ggggtttgcc tgcatcatct atgactgcga cccgatgccc
     6361 cggatacggc cgcccgatcg agcccggctt cgccggccag gcgctctggc agttgcccac
     6421 cacgtagttg atctcggtct gcccgaacat ctcgttgatc gtcacgccga ggtgttcctt
     6481 cgcccactcg aacaccgtca ctcccaccga ctcgcccgcg ctcatgaccg agcgcaggtt
     6541 gagatcgtat ttcccccggg gctgcggcac cgccttcatc atcagcttga gcgcggtggg
     6601 aaacaggaac gaattgcgga tgccgtactt ctcgaggagg taatacgcct tctcggcgtc
     6661 gaaccgcccc cggtagccga ggatcggaat gccgaagagc cacgccggca gcagtgcgtc
     6721 catcaggccg ccggtccagg cccagtcggc gggcgaccag aacacgtcgc cgggacgcgg
     6781 gaagaagtcg tgcgagtgca cgaagcccgg cgcgttgccg agcagcacgc gatgcgcctt
     6841 gagcgcgcct ttcggcgggc cggtggtgcc gctggtgtaa atgatgagcg ccggctcgtc
     6901 ggctttcgta tcgagcggcg tgaaacgcga gctcgcgctt ccgagcaggt cctcccagcg
     6961 cctcaccttg gtctcgttcg cgccgccgac gccgatcaca tgacgcaggt gcgaaagccg
     7021 gtccttgatc gcccagaggt tgacgatggt ggcgggctcc acgatcgcga tcgacgcttc
     7081 ggcgtgctgc atgcggtact cgagcgcgtc gggcccgaag agatgcgaca gcggcagcgc
     7141 gatcgcgccc atctggaaca ccgccatgta ggcgatggcg gtctccgggc gctgcggcag
     7201 gatgatcgcg acgcgctcgc cgcgcttcac gccgagcgcg acgagcgcgt tggagagccg
     7261 gttcgccgcc tgctggatgt cccagaacga gtacgccgcc atcgcgcccg actcgtcctc
     7321 ccagtagagg gcgaagcgat ggcgctcccc ggcccagttg ccgcagcacg cgcgcgccat
     7381 gttgtagcgc tcgggcacgt tccagcgaaa gctcgcgtag agctctgcgt agcggtccgg
     7441 catgcggaga ttctatactt cggccatggc ggccgaggcg ttcgtcttcg atgcatacgg
     7501 aacgctcttc gatgtgtatt cggtcggagc gcgctgcgaa tcgtgctggc cgggaaaagg
     7561 cgcgcaggtc tcccttctct ggcgtgcgaa gcagctcgaa tacacctggc aacgcagcct
     7621 gatgcagcgc tacgcgccct tctccaccgt gacgcgcgaa gcgctcgcgt acgcctgcgc
     7681 agcgctcggg ctggaactga gcgtcgcgca gatggaaggg ctgatggcgg aatatctcgg
     7741 cctcgccctg taccctgacg ttcgtgccac gctggaaaaa ctggattcca ggcgcgcgat
     7801 cctgtccaac ggctcaccgg acatgcttca acctctggtc aagcactcag gattgcggtt
     7861 cgacgccgtg atcagcgtgg acaagctgaa gatctttaag ccggcgccgc aggtctacga
     7921 gctcgccgtc aagaagctca acctcgagaa aaacaggatc gccttcgtct cctccaactg
     7981 ctgggacgcg ctcggcgcca agtcgtacgg cttccgcgtc tattggatca accgggccaa
     8041 ggcgccggtc gaccgcctcg gtttcaaacc tgacgcggtg ctgacttcgc taaatgaagt
     8101 tctgcggtaa ctgcgccgcc ccggtggtga agcgtgtccc gccgggcgac aacctgccgc
     8161 gctgggtgtg cgaccagtgc ggcgagatcc actaccagaa tccccgcctg gtggtgggct
     8221 cgatcccgga atacgagggc aagctgctgc tgtgccgccg cgcgatcgag ccgcgctacg
     8281 gctactggac gctgcccgcg gggttcatgg agaacgccga gacgaccgcg caggccgcgc
     8341 tgcgcgagac gctcgaggaa gccggcgcgc aggtggagct cggcgcgccc ttcaccatga
     8401 tctcggtccc gcacgtgaat caggtgcacc tcttctaccg cgcgcgggtg ctcggcctgg
     8461 aattcaagcc cggcgaggaa agcctggagg tcgcgctatg ggaagagggc gcgattccct
     8521 ggaaggaaat cgcgttccgc accgtcgggc tgaccctcaa gcgctggttc gccgaccgcg
     8581 gccgcggcgc tttcggcttc catgcggagg acctacaata aagtgacaac aaggggaggt
     8641 tggaaaatga aaattctcat cgggttgttg ctcgcgtacg cgcctgccgc gcttgcccag
     8701 gcgccggcgg ctgcgccgaa gatcgacgcc gccaacacgg cgtggatgct cacggcgtcc
     8761 gtgctggtgc tcttcatgac gctgcccggc ctcgcgctct tctacgcagg cctggtgcgc
     8821 acgaagaacg tgctctctgt gctgatgcag tgcttcggga ttacgtgcgt agtgacgctc
     8881 gcctgggtgg cggtcggcta cagcatcgcc ttcggcgatg gcaacgcctg gtggggcggg
     8941 ctcggcaaga gcttcctctc cgggatccag gtgagcacgg tgaagggaag catccccgag
     9001 accgtgttcg cgatgtacca gctcaccttc gccatcatca cgccagcgct ggtcatcggc
     9061 gccttcgccg agcgcgtgcg cttcggcggc atgctgctct tcagcgtgct gtggctgctc
     9121 atcgtgtact gcccgatcgc ccactgggtc tggggcgacg gctggctgat gaaacgcggc
     9181 ctgatggact ttgccggcgg aacggtggtg cacctgaacg ccggcgttgc ggcgctggtg
     9241 tgcgccctgg tgctcggccg gcgccgcggc ttcccggaga ccgcgatgcc gccgcacaac
     9301 atgacgatga ccgtgaccgg cgcggccatg ctgtgggtgg gctggttcgg cttcaacgcc
     9361 ggcagcgccc tcgccgccga cggcgccgcc ggcatggcga tgctcgttac ccacatcggc
     9421 gcggccaccg gcgccttcgc ctggatgctc tgcgagtggc tgcgctacgg caagccttcg
     9481 gcgctcggca tcgtgaccgg catggtcgcc gggctgggca ccatcacccc cgcctcgggc
     9541 ttcgtcgggc cgatcggcgc gctgatcatc ggcggagccg cgggcgtcgc ctgcttcttc
     9601 gcgaccaact acatgaagcg cgcgctcaac gtcgacgatt cgctcgacgt gttcccggtc
     9661 cacggcgttg gcggcctgct cggcacgctt ctcacgggta ttcttgtatc cagtgcattt
     9721 gggggaatag ggtatccaga caaggtgacg atgggcgacc agttcatgac ccaggtcatc
     9781 ggcgtcgtgg cgaccgcggt ctggtccggc gtgatcacct ggatcctgct caagcttgtc
     9841 gacgcgatcg cgggcatgcg cgttgctacg gaggaggaga ccgagggact ggataccgtg
     9901 ctgcacaacg agcagggcta caacctctaa gggcatggag cggcgtggct tcatcaagtt
     9961 ctgcgccgca tcggccgccg ctctcggttc tccgggcgta gcggccgacg cccgggcgca
    10021 gttctattcc agggcccggc tggtcgacga gaaaggcgcg ccgctgcgcg ccgcggcgat
    10081 tccggtccac cgcaatttca tcttccatta ccccttcgcc gcgacgccct gctttctcct
    10141 gaatctgggc aaggcggtga agccggcgca gctcaagacg gtcgccggcg agccctacga
    10201 gtggaaaggc ggcgtcggcg cggtgcgctc ggtggtcgcg tattcggcga tctgcgcgca
    10261 ccggctctcc taccccacca aggacatcag cttcatcagc tttcgcaccg agaagagcgc
    10321 gcgcaaccgc atcgccaacg tgatccactg ctgctccgag cacagccagt acgaccccgc
    10381 cgagggcgcg cgcgtggtcg ccggcccggc gccgcagccc ttggcgacga ttctgcttga
    10441 acatgacttg aagacagacg agcttcatgc cgtcggcacc ctcggcggcg agatgttcgg
    10501 cgagttcttc gacaggttcg gcttcaagct gcagctcgag cacggcacgc cgcgcgccgc
    10561 ggtttccggg tcctgcgtcg tccaggagct ggacaattac tgcaagcaac aggtgaagtg
    10621 ctagcgtggc ttgaaagcaa ggaccccttc ccgccggtcg agcgggcgct gaagaatccg
    10681 aacgggctgc tcgccgcggg cggcgagctc tcggtcgagc ggctgctgga ggcctaccgc
    10741 cacggcgtgt tcccctggta ttccggcgcc gagccggtgc tgtggtggtc gcccgatccg
    10801 cgcatggtgc tctactgcgc ggagctcaag gtgccccgct cgctcgccaa gagcgtgcgc
    10861 aacaagggct tcgaggtgcg catcgacacc gccttcgccg cggtgctgga gggctgcgcc
    10921 gaccggccgc agacctggct cggcgccgac atgcgcaagg cctacctgcg cctgcaccgg
    10981 gcagggtacg cgcactcgtt cgagacctgg cgcaaaggcg agctggtcgg cgggctctac
    11041 ggggtggcgc tcggccgcat gttctacggc gagtcgatgt tctcgcaggc gaccgacgcg
    11101 tcgaaagtcg ccctggtggc gctggtcggg gaactccgcg ggcgtgcatt ccctctcatc
    11161 gactgccaga tgcacacgcc gctcctcgcc tcgctcggcg cccgcgccat cccgcggagg
    11221 tcgtttttgc gcgcaatcgc caccttggta aactaccctg agcccgaggg aaagtggacc
    11281 tcgagggccg gcaacaggag gacgctagag tccgcgtgaa gatcagtgtc aagacttaac
    11341 gacctgccgc acgcagtact gcagttctac gtaaccgcac cgtacccgtg cagctacctt
    11401 cccgaccgca tggcgcgctc gcaggtggcg acgccgagcc atctcatcaa caccgagctg
    11461 tacggcgacc tggtgaaagc cggcttccgc cgcagcggca tcttcaccta ccgcccgcac
    11521 tgcgacgcct gccgggcctg cgtcccggtg cgcatcccgg tggggcagtt cgacgccaac
    11581 cgctcgcagc gccgcgcctg gaagcagcat tccacgctca ccgcgcaccc gcagccgctc
    11641 aagttccggc tcgagcacta cgcgctctac ctgcgctacc agtccaagcg ccattcgggc
    11701 ggcggcatgg acaacgacag ccgtgaccag tactcgcact tcctgctgca gagccgcgtc
    11761 gacacgcggc tcatcgagtt ccgcgacgcg ggccagctcg tgatggtgtc gatcgtcgac
    11821 tacctgcccg acggcctgtc ctcggtctac accttctacg agccggaccg tcgcaccgcg
    11881 agcttcggca cctacaacgt gctgtggcag atcgagcagt gccgcgccct cgccctgccc
    11941 tacctctacc tcggctactg gatccgcgag agcccgaaga tggcctacaa gtccaagttc
    12001 gagccgatcg agggctttgt cggcggcacc tggcgccgcc tcgggcggcg gctgaccgaa
    12061 gagtccaagg aagagaagcg ggcgtgagcg ttcgggcgag agggtcttga acctctaccg
    12121 gctcgcccga ccgctcctct tctcgctaga ccccgaaaca gcccataaag tcggtctcaa
    12181 cctggccgcg ctcgcgcccc ggcggcgcct ccattgcccg gtcagggcga tgggcctgga
    12241 attcgcgaac ccggtcggcc tggcggccgg cctggacaag gacgccgcgc acatcgacgc
    12301 gctcgcgcgc atgggcttcg gcttcatcga ggtcggcacg gtgacgccgc ggccgcagcc
    12361 gggaaacccg cgcccgcgcc tgttccgcat cccggagaag gaggcgatca tcaaccgctt
    12421 cgggttcaac aacgtcggcg tcgatgcgtt cctggagaac gtggcgcgcg cccgctggcg
    12481 cggtgtcctc ggcatcaaca tcggcaagaa cgccgatacc ccggtggaac gggcgatcga
    12541 cgactacgag ctgtgcctgc agaaggtcta cgccgcggcg tcctacgtga cgatcaacat
    12601 ttcctcgccg aacaccgccg ggctgcgctc gctgcaggag cgcgattcgc tcgaggacct
    12661 cctcggaaag ctgagcgccg cgcggcagga tctgtcccgg cggcatggca agcgcgtgcc
    12721 gctcgtcctg aagatcgcgc ccgacctcgc gccggaggac atcccctcca ttgccgcctc
    12781 ggtgcagcgc cacgccatcg acgggctcat cgccaccaac acgtcgctct cgcgggaggg
    12841 cgtggcgaac ctgccgcacg ccggcgaggc cggcggcctt tcgggcgcgc cgattcgcga
    12901 ccggtcgacg cgggtcctga aggagttttc cgccagcctt ccgggaacca cgctgatcgg
    12961 cgcgggcgga attctcagcg gccgcgacgc cgaggagaag ttctccagcg gagcggctct
    13021 ggtgcagctc tacacggggc tggtctaccg tgggccgggc ctggtctcag aatgcgtatc
    13081 ggcattccgc gcgaagtaaa ggacggcgag caccgggtcg ccttgtcgcc ctcggggatt
    13141 cgcggcctcg gcgacgtcgt cgtcgagccc ggcgcgggac agggcgtggg attctccgat
    13201 gacgactacc gccaggccgg agcgcggctc ggcgatccct gggactgcga tctcgtagta
    13261 aaggtcaagg aactgcagga atcggagtac gcaagaccgc ggccgggcca gaccgtgttc
    13321 ggcttccagc acttcggccc tgacccggat ctgctggacg cggcgctcgc ctcgggcgcg
    13381 acctacctcg cctacgagac cgtcggccag gccggcggcc ggctgccgat cctcgcgccc
    13441 atgtccgcca tcgccggaaa gctcgcggtg caggtgggcg cgtggtgcct gctcaagcag
    13501 aatggcggca gcggggtact ccttccgggg ctggacggag tgccccccgg caaagtcgtg
    13561 atcctcggcg ccggcaacgt cggctcgaac gcgcttgccg tggcctatgg gctcggcgcc
    13621 cgcgtcgccg tgttcgccaa gagcgagaag cgcttccccg ggttgaaagc aaagtacccg
    13681 cacgctggct tccacgtcgg ccccctcggc caaacgctcg ccgatgccga tctcgtcatt
    13741 ggcggcgtgc tcacgccggg cgagatgtcg ccgaagctga tcgatcgcaa gaccttgaga
    13801 aacctgcgga ccggctcggc cctggtcgac gtcggaatcg accagggcgg gatcgcggag
    13861 acttcgcgac cgacctcgca tagcgaaccg atctatatcg aggaaggcgt ggtgcactac
    13921 tgcgtgccga acatgcccgc cgcgtgcgcg cgtaccgcga gccttgcgct cgagcgcgcc
    13981 gttcggccct tcgtgctgaa gatcgtgaaa ggcgagatcg actccgacct cgcgaccgcc
    14041 atccaggtca aggcgggaaa agtgacgcac gagcatttgg cgcgcgatac cggaagggca
    14101 tactcgaggc tatgaaacgg caggccgttg cgctcatcga cgagagccgc tgcatcggct
    14161 gcacgctgtg catcgacgcc tgcccggtcg atgccatcgt tggggcgtcg cagctcatgc
    14221 acacggtggt cgagccctgg tgcatcggct gcgagctttg cgtcccgcct tgcccggtcg
    14281 actgcatcgc catggcgccg gcgcgcggag aatggaatgc gcagctcaag cgggccgccg
    14341 gcgatcgggg acgcgctcga gcgaaaagaa tgaagcaggc ccgggtcccg gcacgcgtcg
    14401 tcgatcggaa gaccatcctg gctgcggcac tcgagcgcaa gaaactgcgc taggccgcca
    14461 tgctcggaac ccaggatctc ctgctgttcg tgctttcggc tctgctgctg aatatcacgc
    14521 cgggtccgga cacgctctac atcgtcggcc gcagctcgac ccaggggctg aaggccggcg
    14581 ttatcgccgc gctggggatc agcgcgggaa tcatggtgca tgtcacggct gccgcgctgg
    14641 gcctctcggc catctttacg gcttctgcaa ccgcctttgc cgtgctcaag ctggcggggg
    14701 cggcttacct cgtctacgtc ggcatttcgc tgctgcgcac gcgcgtcgtc gagacgcgcg
    14761 cgcccgaacg cgcggcgctg cgcaccgtat ttatccaggg attctggacc aacgtcctca
    14821 atcccaaagt cgccctgttc tttctcgcat tcctgcccca gttcgtggac ctcgacgcac
    14881 cgggcaaggt gattcccctg cttttcctgg gactgctgtt caacgtcaac ggcactctct
    14941 ggaacctgtt cgtggcctgg tcttcggctc gagtcggccg cgccgtattg gcgcatggtc
    15001 cggcggcgac ctggttcaac cgctccgtcg gtgcgctctt catctatctc ggcgtgcgac
    15061 tcgccctgac ccgggaatga atccgcagaa gcggcgcgag atctttcgcc gcctgcaggc
    15121 ggcgaacccc acgccgacca ccgagctcga gtaccgcaca ccgtacgagc tcctggtcgc
    15181 cgtcgtgctg tcggcgcagg cgaccgacaa gtcggtgaac ctcgcgacga gcaagctgtt
    15241 ccccgccacc ccggagaagc tggtgcaact gggcgtcgcg ggtgtcgagc cgtacatcaa
    15301 gagcatcggc ctctaccgca ccaaggcgaa gaacgtggtg gcgctgtcca ggatgctgct
    15361 cgacgagcat tgcggcgcgg tgccgcagaa tcgcgaagcg ctcgagcgcc tgccgggcgt
    15421 gggacgcaag accgcgaacg tggtgctgaa caccgctttt ggccagccga cggtggcggt
    15481 cgacacgcat atctttcgcg tttccaaccg caccggccta gccccgggca aggatcccct
    15541 ggaggtggaa gagaagctgc tcaagttcac cccgcccgaa tacctcaaga acgcgcacca
    15601 ctggctgatc ctgcacggac gctacgtctg cctcgcccgc aagcccggct gcccccagtg
    15661 cctgatccgg gacttgtgcg aattccggcc gaagaccaag gatgtttaat ccaagccggg
    15721 aggaggttcg ggagacgttc ttcgctgcct ggcgcaagta ccgcgccggc cagccgctcg
    15781 ccggaatcga ggcgctggcg ctcgaggtga tcctgctgca cccggagtac cacgatgccc
    15841 tggcgagccc cgatcgcagc cgggacaagg actatttcga cgaatcaaac ccgttcctgc
    15901 acatgagcct gcacgtggcg ctggaggagc agctgtcgat cgatcagcca cacgggatcg
    15961 cacagcgctt tcaggctctg gtttcgaggt ttcaggaccg gcaccaggcg cttcaccagg
    16021 cgctcgagtg cctcgcggaa acggtgtggc gcgcgcagcg agacggcgcg ccgccggatg
    16081 cggccgcgta cctcgattgc ctcgcggcgc gcgcgctacg caagtagttg cgcgatgaca
    16141 ggtttggaac tgagccgtca ggactaattg cgctattcgc gccgggaata ttcgcgggcg
    16201 catgatggct ccacaacgaa cgcggagacc gccatggcca cgaccgaacg cctatcccta
    16261 ccggcacttt tcatggcggc atgcgcgatc gccgcgatcg catggtcgtc acccgcgcgc
    16321 accgcggacg attccgactc catcatcctc gtcgcgaagc gccagctcca ggacaagctc
    16381 tacggctcga cgatcctcat tgcgcggccg atcggcgccg atcgccacgt cggattcatc
    16441 atcaacaagc cgacgcaggt gacgctcggc aagctgttcc cgacgcacga gccttcccag
    16501 aaagtggccg accccgtgtt cctcggcggc ccggtgagcc ccgaggtgat cttcgcgctg
    16561 gtgcagggca aggaaagccc cggcggccgc tcggtcaaga tcctcgacga cctgtacctc
    16621 gcgatcgaca gcgacgtcgt cgaccgcgtc atcgagaagc agcccgcgaa ggcgcgcttc
    16681 ttcgccggca tggtgctgtg gcgcccgggc gagctccacg aggagatcaa gaaaggcctc
    16741 tggtacgtga acgacgccag ggccgacctc gtgctgcgca agccgaccga gagcatgtgg
    16801 gaagagctcg tcggccgctc cgagcgcaaa gccaacgcga tctaggaacc taccgctggt
    16861 cgtgcaggtt ggacttctgg ctggaaaacc acgccgccag gtcctcgatg tccttcttcg
    16921 atagctgcga cgcgaacccc ttcatgatcg ggttgttgcg ccggccgagc ttgtagtccg
    16981 tcaaggcgcg caccaggtag tcgtagtgct gccccgcgag gatcggctgc gtcggatccg
    17041 agggcttgtt gccgtcggga ccgtggcacg cggcgcacac ctgggcggct ttctcttttc
    17101 ccgacgccgc attgcctttg gcgtgcgcag gaactgcaaa caccgcagcg gcgagagcga
    17161 cgagcagacc cctcacttgc ttcccccgta atagtcggcg agctgcttca tgtcctcttc
    17221 ggacaggctc gcggcgatgc cgcgcatcga cgggtgcgag cgctcgcccg acttgtaggc
    17281 cttgagcgcg ttgacgaggt agccgggctg ctggccggcg atcttgggca cgtggtacac
    17341 gtcggggaac gcggtcttgt agcctgggat gccgtggcag ccgatgcaca tggcgtgcgc
    17401 gttctccatt ccctgcccgg tcttcgcggc ggcagcgggc ttcttttcct gcgcgtgcgc
    17461 gccgaacgcc agcgcaacgc caagcaggat gaaaccagca attttcagaa tggccccgga
    17521 catccgcggc gaattctagc gtaaaaaaag cccgctgcta gaatcggccg catgcgtttc
    17581 aagggatcgc agtcctacgt ctcgaccgaa gacctgacgc tcgcggtcaa cgcggcgatc
    17641 acgctgcagc ggccgctgct cgtcaagggc gagcccggca ccggcaagac catgctcgcg
    17701 ctcgaggtgg cccgctcgct cggcgtgccg atcctcgagt ggcacatcaa gtccaccacc
    17761 aaggcgcagc agggcctcta cgagtacgac gcggtgagcc ggctgcgcga ctcccagctc
    17821 ggcgacaagc gcgtgcacga catccacaac tacatcgtga agggcatgct gtggcaggcg
    17881 tttaccatgg accagccctg cgtgctcctg atcgacgagg tcgacaaggc cgacatcgag
    17941 ttcccgaacg acctcctgcg cgagctcgac cgcatggagt tctacgtcta cgagacgcgc
    18001 gagctcgtga aggccaagca ccggccggtg gtgttcatca cttcgaacaa cgagaaggag
    18061 ctgcccgacg cgttcctgcg gcgctgcttc ttccactaca tccgtttccc ggacaaggag
    18121 acgatggaga agatcgtcgc ggtgcacttc ccgaacctca agaaatcgct cttgcgcgag
    18181 gcgatggagg tgttcttcga ggtgcgcgag gtgcccggcc tgaagaagaa gccctccacc
    18241 agcgagctcc tcgactggct gaaacttctc ctcgccgagg acatcccgcc ggaggcgctg
    18301 cgctccaagg accgcaagac catcatcccg ccgctgcacg gcgcgctcct gaagaacgag
    18361 caggacgttc atctgttcga gcggctggtg ttcatgaccc gcgccaaggg cgggtagccg
    18421 accggctaat cgccggcgct tggctgtgct ggcggcgcga attgcgccac cgcccggagc
    18481 aactcgtcgg ccatcaccgg cttcaccagg tggccggcgc cgagctgttt cgcgcggtcg
    18541 ccgacgctct tgttcaccgt aaggaagatc accgggatgt ggcgcgtcgc cggatcggcg
    18601 cgcagcgccg acacgaactc gtagccgtcc atgaacggca tgtccacgtc gcagaggatc
    18661 acgtcgggcg tgcgctccac gatcacgcgc ccggcggcca ccgcatcctc ggccgcgaag
    18721 acctcgtagc ctgccttgcg cagatgcagg tcgaggaggt agcgaatgaa gcgatcgtcg
    18781 tcgatgacgg cgaccttcgc cttaggcgca ggggtaaatt gcctcgatcc gttcatggcg
    18841 ggccaagcta ccacagctcc ttgcagagct ctttgcgcct gacagaactt gtggcgggcc
    18901 gcgtggaata agccacagtc ctggcgccac cggcgcgggc aagatgcgca agtgccgaag
    18961 gacttcaatc tagggctggt attcctgtcg tacctcgtgg cggtgaccgc gtcgcacgtc
    19021 acgctgctcc tcgccgcgcg cgtgcgcgat ccgcacgcca ccaactggaa gctgtgggtc
    19081 gtgtgcggcg gcttcgcgat gggcatcggc atctggtcga tgcatttcgt cgccacgctg
    19141 gcgctcaagc tgccgatccg cgtgctgtac gacctgtcga tcaccgcgct ttcctgggtg
    19201 ttcgcgatcg tcgcgtgcgg cgcggcgttc atcgtcctgc gcaggctcac gggcaagcat
    19261 cgcgagttcc tcctgccggg cgcgctgatc ggcatcggca tcgcctcgat gcactacacc
    19321 ggagacgcgt cgatgcgcct ctcccccggg atccggtacg acccggcgct gttcgtcgcc
    19381 tcggtcctga tcgccatcgg cgcggccacc gcggcgctgt ggatcgcgtt ccacctcgcc
    19441 aagcagcgcg cggtgtggac gaacttcggg gccgcgttgg tgatgggcgc ggccgtggtc
    19501 ggcatgcact tcacggcggt ggcggcggca gagttccatc ccgcgtcgat atgcctcgcc
    19561 tccgggctgc ggctcgacga ggagtggatg gcctacacca tcggcgggtt cacgttcgtc
    19621 atcctgaccg ccaccatgct cctttcggcg tacgacgcgc gcctctcctc ggccattgcc
    19681 ggcggcgcgg agaggctgcg gcaggcgaac gagcagctgg agatgcgcgt gcgcgagcgc
    19741 acggaacagc tgcgccgggc gcacgatgcg gcgcgcgccg ggtcggaagc gaagagcgcc
    19801 ttcgtcgcca ccatgagcca cgagatccgc acgccgctga acgcggtcat cggcatgctg
    19861 gagctcttct cctacagcaa gctgagctcc gaccagcgcg aaatgctgtc ggcggcgcgc
    19921 gaatcgagcc gctcgctcct cgcgctgatc gacgacgtgc tggacttctc caagatcgag
    19981 cgcggcaagc tcgaggtccg gccggagccc tgctccgtgg cgcgcctggt ggacggcgtg
    20041 aacgcgacct tcgagcatgt ggcgagcggc aaggggctgc ttctcacgcg gcgcatcgat
    20101 ccggcgctgt ggccggcgca cctcgtcgac ccttcgcgcc tgcgccagat tctcaccaac
    20161 ctcgtcagca atgccatcaa gttcacgcac gccgggacgg tcaccctgga ggtgaaggtg
    20221 ctggcggcgg gggaaggacg ccagaaattc tgcctcagcg tgcacgattc aggcatcggc
    20281 atgagcgccg acacgcaggg gcgcctgttc gagccgttcc agcagggcga atccgagacc
    20341 agtcggcgct acggcggcac cggcctcggg cttgccatct cgcgccagct gatcgagctg
    20401 atgggcggca ccatccaggt caccagcacc gaaagcctgg gcacgcgcgt ttcagtgacg
    20461 ctctcagccg aacaggcgag cgtcggcgca atccgcgacg acgggcagac tcccagcggc
    20521 gcgcgcgctc ccgtcccggt ctctgcaccc gccgggacca agcttttgat cgtcgacgac
    20581 catccgatca acctcgccat gctcaagcgc cagctgaagg tcctgggcct cgaggccgac
    20641 accgcggcga gcggcgtcga ggcgctcgcc aagtggcgcc gcgaaggcca caacctcgtc
    20701 atcaccgacc tgcagatgcc cgagatggac ggctacgcgt tcgcgcgcgc cctccgcgcc
    20761 gaccgggatc aggaaaacat gcccaagccg acggtgatcg cctttaccgc caacacgcag
    20821 agcgatgcgc tcgagcggtg catcgacgcg gggatggacg actatctcac caagccggcc
    20881 gagctcgtga cgctgcgcgc aaagctcgcg cagtggctgg gcagcgacgc gccgctccgg
    20941 accgcgccga gcacccggcc cgccagcgcg ccggacggga atccgatcaa tcgtgcgcgc
    21001 atccagcaac tcgccggcag tccgcagggc atcgccgaag tgctggccga gctcgagtcg
    21061 ggcgtgcgcg ccgacatcgc cgccctgcag gtcgcgctcg agagcgccaa cggcgaagcg
    21121 ctgcggcgcg ccgcgcaccg catcaagggc agcgccctaa ccatcggtgc cgagcgcctg
    21181 gcggcgctgg ccgcaggcgt cgtagacgcg cccgccggcc tcgccgagcc gcagctcatg
    21241 agcagcgcgc gcgggcttct cgacgagctg gagcgagttc tcctatccgc acgggtgcat
    21301 cgcagcgcgc cggcttcgta gttgccagcg gccgatcttt cacttccgca cgcccacggc
    21361 cgggcggttc ttgcgataag agtttttcag cgcgatcctg ccgtctcgaa aggtgaacag
    21421 atcgcaacca tggacttcga cgcgcgtgcc gtcgcgccgc gttcccgtga acgtccactc
    21481 cgatacccct cgctctccac acaccatatg gcgcgcattg ccccagtggg cgtcggggaa
    21541 agtcgcccag acctccgcga agccggctcg gacggcgtcg tggcctgcgt atcgggtgcc
    21601 gcagacgtcg ggtcctgcag aggcttcgaa aacgcaatcc tcggtcatga atgacatgag
    21661 agcatcgacg tcgtgccgat tccatgcatc ggcaaaggct tgcaagacct ccggggtcac
    21721 ttcatcgatc acattggcca tggtctggcc ctcctttcaa ttgcggatcc ggacaacggg
    21781 gccgatgacg attcactctg cacgttggca ggtcggccgt cgtgccatgg tagaaccgct
    21841 gcgaactgtt aggcattctg cttggaaagc actagctgcg gcattgggtg aatctcaaac
    21901 ccaaagccaa catg
//
LOCUS       DATBSZ010000083         3349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1787556, whole genome shotgun sequence.
ACCESSION   DATBSZ010000083 DATBSZ010000000
VERSION     DATBSZ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787556"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="VIG70_05550"
     CDS             complement(<1..128)
                     /locus_tag="VIG70_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY8250052.1"
                     /translation="MKEPVDFYFDFSSPYGYLASHKIEPLAAKYGRGVTWRPMLLGA"
     gene            complement(125..1246)
                     /locus_tag="VIG70_05555"
     CDS             complement(125..1246)
                     /locus_tag="VIG70_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8250053.1"
                     /translation="MSAQAEEIRQAVRRFAREQLAPNAAQWDREKLFPKDALKGLAAM
                     GLYGVAIAEEWGGAGLDYTALAVACEEIAAGDCATATIVAVNNLVAGILSGYADKNQK
                     EKYLRPLAQGKLLGAFALTEPQVGSDASAITTRAEKQGAHYVLNGVKQFITSGKNADI
                     AVVFAVTDKGAGKKGISAFLVPTSAPGYVVARIEEKTGQRASDTAQIVFENCKAEHLL
                     GGEGVGYRIALANLESGRINVAAQATGVARAALEAAIAYARERKSMGKHLIEHQAVNF
                     RLADMATQVEAARQLYLHAARLRDEGQPCLKEASMAKLFASEMAEKVCSDAIQIHGGY
                     GYVSDFPVERLWRDVRVTKIYEGANDIQRLVIGRALEEG"
     gene            complement(1230..1625)
                     /locus_tag="VIG70_05560"
     CDS             complement(1230..1625)
                     /locus_tag="VIG70_05560"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY8250054.1"
                     /translation="MKRPLQQLLDRSRFLRPYGFRVVTANKDKCTLELPHKAALERPG
                     GIINGPALMAAADCAMWLAIKARIGVENDALTSELNTAFLAPAKGEHVYCTARILKMG
                     RRRIYGVAECHGRKGNLFSHHTLTYVRAG"
     gene            complement(1622..2806)
                     /locus_tag="VIG70_05565"
     CDS             complement(1622..2806)
                     /locus_tag="VIG70_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY8250055.1"
                     /translation="MNPDPVVIVGLARTPMGGFQGELKGFAAPELGAAAIRASVERAK
                     IKPEEVQEVIMGCVLPAGQGQAPARQASLGAGLPLATGCTTVNKMCGSGMKAAMFAHD
                     LLLAGSSEIIVAGGMESMTNAPYLLPKARAGLRMGHGQVIDHMFYDGLEDAYDKGRLM
                     GSFAEDCASKYQFSRQAQDDFAIQSLKRAQQANREGWFAWEITPLAIKVGKEEKFVEI
                     DEQPFKANLEKIPTLKPAFRKDGTVTAANSSSISDGAAALVMMRRSSAEKRGLAPLAV
                     VVGHATHAQEPGWFTTAPVGAIRKLFEKTGWTAKQVDLYEINEAFAAVTMAAMKEHGL
                     PHDKVNVHGGACALGHPIGASGARLVVTLLGALRKYGLKRGVASLCIGGGEATAMAVE
                     LL"
     gene            complement(2803..3207)
                     /locus_tag="VIG70_05570"
     CDS             complement(2803..3207)
                     /locus_tag="VIG70_05570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY8250056.1"
                     /translation="MFETARGTVHSWQCDHMGHANLRAYGEFFEQALWHVFHRIGLTP
                     SVLRGDAIRMAGVQQCINYKKELLPGDLVVVHSRLAEFKDRSLKMVHEMQHVESGELC
                     ATCELTAVCIDAQTRKPRTFPAEIAARAKEIL"
BASE COUNT          501 a         1105 c         1132 g          611 t
ORIGIN      
        1 gcgcccagga gcatcggacg ccacgtgacg ccgcggccgt acttcgcggc gagcggctcg
       61 atcttgtgcg aggcgaggta gccgtaaggg gacgagaagt cgaagtagaa gtcgaccggc
      121 tctttcatcc ttcctccagg gctcgtccga tgaccaatcg ctgaatgtcg ttcgcgcctt
      181 catagatctt ggtcacgcgc acgtcgcgcc acaggcgctc gaccgggaag tcggagacat
      241 agccgtagcc gccgtggatc tggatcgcgt cggagcagac tttctcggcc atctcggagg
      301 cgaagagctt cgccatcgag gcctccttga ggcagggctg gccctcgtcg cgcagccggg
      361 ctgcgtgcag gtagagctgg cgcgccgcct cgacctgggt cgccatgtcg gccaaccgga
      421 agttcaccgc ctggtgctcg atcaggtgct tgcccatgct cttgcgctca cgcgcgtagg
      481 caatcgccgc ttcgagcgcc gcgcgcgcca cgccggtcgc ttgcgcggcg acgttgatgc
      541 gccccgattc gaggttggcg agcgcgatgc gatagccgac gccctctcct ccgagcaaat
      601 gctctgcttt gcagttctcg aaaacgatct gcgcggtgtc ggaggcgcgc tggccggtct
      661 tctcctcgat ccgcgctacg acatagccgg gggccgaggt cgggacgaga aaggcgctga
      721 tgcccttctt gcctgctccc ttgtccgtca ctgcaaagac gacggcgatg tcggcgttct
      781 tgccggaggt gatgaactgc ttcacgccgt tcagcacgta gtgcgcaccc tgcttttcgg
      841 cccgcgtggt aatcgcggag gcgtcggacc cgacctgcgg ctcggtgagc gcgaaggcgc
      901 ccagcagctt tccctgcgcg aggggccgca ggtacttttc cttctggttc ttgtctgcgt
      961 agccggaaag gatcccggcc accaggttgt tcacggcgac gatggtcgcg gtggcgcaat
     1021 cgccggcggc gatttcctcg caggcgacgg cgagcgcggt gtagtcgaga ccggcgccgc
     1081 cccattcctc ggcgatggcg acgccgtaga ggcccatggc agccaggcct ttgagggcat
     1141 ctttcggaaa aagcttctcg cggtcccact gggcggcatt cggcgcgagc tgctcgcgcg
     1201 cgaagcgccg caccgcctgc cggatttcct cagcctgcgc ggacataggt gagcgtgtga
     1261 tggctgaaaa ggttgccctt gcggccgtgg cattcggcga cgccgtagat gcggcggcgt
     1321 cccatcttca ggatgcgcgc ggtgcagtag acgtgctcgc ccttggcggg agcgaggaac
     1381 gcggtattca gctccgaggt aagcgcgtcg ttctcgacgc cgatgcgcgc cttgatggcg
     1441 agccacatcg cgcagtcggc cgccgccatc agcgccgggc cgttgatgat gccgccgggc
     1501 cgttccaagg ccgccttgtg cggcagctca agggtgcact tgtccttgtt cgccgtgaca
     1561 acccggaatc cgtagggccg caagaagcgg gatcggtcga gcagttgctg gagcggtctt
     1621 ttcaaagcag ctcgaccgcc atggcggtag cctcgccgcc gccgatgcac aggctcgcca
     1681 ccccgcgctt cagcccgtac ttgcgcagcg cgccgagcag tgtcacgacg aggcgcgcgc
     1741 cggaggcgcc gatggggtga ccgagcgcgc aagcgccgcc gtgcacgttc accttgtcgt
     1801 gcggcagccc gtgctccttc atggccgcca tggtgaccgc cgcgaaagcc tcgttgatct
     1861 cgtagaggtc gacctgcttt gccgtccagc ctgtcttctc gaagagcttt ctgatcgcgc
     1921 cgaccggcgc ggtcgtgaac cagccgggct cctgcgcgtg cgtggcgtgg ccgacgacca
     1981 ccgcgagcgg cgcgaggccg cgcttctcgg cgctcgagcg acgcatcatg acgagtgccg
     2041 cggcgccgtc ggagatcgag ctcgagttcg ccgcggtcac cgtgccgtcc ttgcggaacg
     2101 cgggcttgag ggtcgggatc ttctccaggt tcgccttgaa cggctgctcg tcgatctcga
     2161 cgaacttttc ctccttgccg accttgatcg cgagcggcgt gatctcccag gcgaaccagc
     2221 cttcgcgatt ggcctgttgt gctcttttca aggattgaat cgcgaagtcg tcctgcgcct
     2281 gccgggaaaa ctggtacttg gaagcgcagt cctcggcaaa gctccccatc aaccgccctt
     2341 tgtcatacgc gtcctccagg ccgtcgtaga acatgtggtc gatcacctgg ccgtggccca
     2401 tgcgcaggcc cgcgcgcgcc ttgggcagca ggtacggcgc gttggtcatc gattccattc
     2461 cgccggcgac gatgatttcc gaagaacccg ctagcagcag atcgtgggcg aacatggccg
     2521 ctttcatgcc ggagccgcac atcttgttca ccgtggtgca gccggtggcg agcggcaatc
     2581 cggcgccgag cgaagcctgg cgcgcgggcg cctggccctg gcccgcgggc agcacgcagc
     2641 ccatgatcac ctcctgcacc tcctcgggct tgatcttcgc gcgctcgacc gaagcgcgga
     2701 tggcggcggc gccgagctcg ggagcggcga agcccttgag ctcgccctgg aagccgccca
     2761 tcggagtgcg cgccaggccg acgatgacta cgggatcggg gttcatagga tttcctttgc
     2821 tctggccgcg atttccgcgg ggaaggttct gggcttgcgg gtctgggcat cgatgcacac
     2881 cgcggtgagc tcgcaggtgg cgcagagctc gccggattcg acgtgctgca tctcgtggac
     2941 catcttcagg ctgcgatcct tgaattcggc gaggcgtgaa tgcaccacga ccaggtcgcc
     3001 gggcagcaat tccttcttgt agttgatgca ttgctgcacc ccggccatcc ggatcgcgtc
     3061 accgcgcagc accgagggcg tgaggccgat gcggtggaac acgtgccaca gcgcctgctc
     3121 gaagaattcg ccgtacgcgc gcaggttggc gtgccccatg tggtcgcact gccaggaatg
     3181 gacggtgccg cgcgcggtct cgaacatcag cttttgagca acctggcggt ggcctggacg
     3241 gcggcgagcg tttcggcctc cagcgccgcg acgagccttg cggcgggaag cgcgcgcgaa
     3301 agcggcgccg cctgcccggc ccacatcgcg acgaagtccg gcctgccgg
//
LOCUS       DATBSZ010000084         4815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2403591, whole genome shotgun sequence.
ACCESSION   DATBSZ010000084 DATBSZ010000000
VERSION     DATBSZ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2403591"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1563)
                     /locus_tag="VIG70_05575"
     CDS             complement(49..1563)
                     /locus_tag="VIG70_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEY8250057.1"
                     /translation="MEIPREYNAAVDLIERHLAAGRGGKIAYIDDSGSTTYAELAERV
                     DRATSALRGLGIKREQRVAIAMLDSADWVALFLGAIKAGVVPVALNTLLTPADYEYQL
                     NDSRARALFFSSALAKSFDGIQPKCRHLKYVVNDAQLKDLLATADSKAEPAGTMRDDM
                     CFWLYSSGSTGAPKGTVHLHSHLIDTADLYAKPVLGIRESDVVFSAAKLFFAYGLGNS
                     LTFPLAVGATTILMAERPTPDAVFKRLVSGKPTIFYGVPTLYAALLASSNFPKKDQLS
                     LRICTSAGEALPAEIGRRWTERTGAELLDGIGSTEMLHIFLSNRPGDVRPGTTGKPVP
                     GYQLRLVDDQGNVVTKPGELGELQISGPTSAIMYWNQREKTKNTFQGPWTRSGDKYSL
                     DQDGYYAYGGRSDDMLKVSGIYVSPAEVEAALVSHEAVLEAAVVGAADEQKLIKPKAF
                     VVLKPGKSPSDTLKTALQQHVKDKLAPYKYPRWIEFLGELPKTATGKIQRFKLR"
     gene            complement(1530..2177)
                     /locus_tag="VIG70_05580"
     CDS             complement(1530..2177)
                     /locus_tag="VIG70_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8250058.1"
                     /translation="MFRHGLLPQLLVFEAVARLGSVTRAAAELHLAQPTVSLQLKKLA
                     ETLEAPLFEQRGRRLHLTGAGHALRETCEELLQCLARAETRLAAWRQPKKENLLLAAE
                     PEARAVAARMLADFCAHHPGVQATLHVGERADLLARLAAGADDVYLFALEVEGLPEER
                     RWSLAHPKGRRLGPAAERFFREALRLDAEAGAANNAPASEEEQEQWRSPGNTTPR"
     gene            2418..3098
                     /locus_tag="VIG70_05585"
     CDS             2418..3098
                     /locus_tag="VIG70_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY8250059.1"
                     /translation="MFTPEFWSALAAIVVIDLVLAGDNAIVIALAARNLPKALQRRAV
                     LWGTFGAVALRASLTVAVLWLLEIPGLMLAGGILLAWIGYRLLAGSENGGEHEVTPAR
                     SFWGAMRTIVVADAVMGLDNVLAVAGAAHGSAVLVVAGLVISIPIVVWGSTLILHWIE
                     RFPALLYLGGAVLAWTAAKMIADEPLYQEWLDERAGIKSLLFIVITGGVLGLAWLRNR
                     RATKEVLQ"
     gene            3095..3595
                     /locus_tag="VIG70_05590"
     CDS             3095..3595
                     /locus_tag="VIG70_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8250060.1"
                     /translation="MKVLVPVDASEAALAPIGYLETLARSGVPLEVLVMNVQPRFHRH
                     VARFTSRAARDGVRAERSRAAMARAIEALSLRNIAFRAFAEVGLPAPRIAAVAEREQV
                     DEVMMGVGRHPAWLRWVNPSIAQGVMERTDIPVTVLARGRAGALERYALPAGVAGLAA
                     LLLAGE"
     gene            3598..4062
                     /locus_tag="VIG70_05595"
     CDS             3598..4062
                     /locus_tag="VIG70_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8250061.1"
                     /translation="MRALIPVLDSVNALPAVRYVIRESLSGERHDVRLLDVRSPLGHA
                     LEGWLTGDALQPACELLDRFRVRHTVQRIAAQDRADAIVAAALSEGPDAIVLGTARYR
                     SATRMSEDGVIQKLLEVAPLPVLLVTGREVSRLERYGIAAGLGATLGLIFLG"
     gene            complement(4059..4544)
                     /locus_tag="VIG70_05600"
     CDS             complement(4059..4544)
                     /locus_tag="VIG70_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8250062.1"
                     /translation="MIKKLHHNAYRCRDSEETRAFYEDFLGLKLACTLDIEETMTGRK
                     TRTLHTFYEMGDGSYLAFFEAPDRPFEFKDQHDYDLHIALEVEEKALAPMMAKGKSRG
                     IETRGVSNHGFIHSIYFRDPNGYVIELTAKTPGHAEATDPKRNSARAKLDRWQASKAA
                     A"
     gene            complement(4553..>4815)
                     /locus_tag="VIG70_05605"
     CDS             complement(4553..>4815)
                     /locus_tag="VIG70_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY8250063.1"
                     /translation="RVWLGILDKDLIGPSKKFLCGSTPTIADYFAAPILTLGDLLRCD
                     YAAYPNIRRWLATMKALPSWPKVNEAFHGYAHAIKDQPFHTP"
BASE COUNT          701 a         1574 c         1706 g          834 t
ORIGIN      
        1 gcgaattgcc accttgcggc aggacgtgag cgacgcgact gactttcctc atcgaagctt
       61 gaacctctgg attttgccgg ttgcagtctt aggcagctcg ccgaggaatt cgatccatcg
      121 cgggtacttg tagggcgcga gcttgtcctt gacgtgctgc tgcagggccg tcttcagcgt
      181 atcagacggc gacttgccgg gcttcaggac cacgaacgcc ttgggcttga tcagcttctg
      241 ctcgtcggcg gcgcccacca cggcggcttc gagcaccgct tcatgcgaga cgagtgcggc
      301 ttccacttcg gcgggcgaca catagatgcc gctcaccttg agcatgtcat ccgagcggcc
      361 gccgtaggcg tagtagccgt cctggtcgag gctgtacttg tcgccgctgc gcgtccacgg
      421 tccctggaag gtgttcttgg tcttctcgcg ctggttccag tacatgatcg ccgaggtcgg
      481 gccgctgatc tgcagctcgc cgagctcgcc cggcttggtg acgacgttgc cctggtcgtc
      541 caccaggcga agctggtagc ccggcaccgg cttgccggtc gtgccggggc gcacgtcgcc
      601 gggccggttg gaaaggaaga tgtgcagcat ctcggtcgag ccgatgccgt cgaggagctc
      661 ggctcccgtg cgctcggtcc agcgccgacc gatttccgct ggaagagcct cgccggcgga
      721 ggtacagatg cgcagtgaaa gctgatcttt cttcgggaag ttggacgaag caagcagtgc
      781 cgcgtacagg gtcggaacgc cgtagaagat cgtcggtttt cccgaaacca gacgcttgaa
      841 aacagcatcg ggcgtcggac gctccgccat gagaatcgtg gttgcgccca cggcgagcgg
      901 gaaggtgagc gagttgccca ggccgtaggc gaagaagagc ttcgccgcgg agaacacgac
      961 gtcggactcg cggatgccga ggaccggctt ggcgtagagg tcggccgtgt cgatgaggtg
     1021 cgagtgcagg tgcaccgtgc ccttgggcgc cccggtcgag cccgacgagt aaagccagaa
     1081 gcacatgtcg tcgcgcatcg tccccgccgg ctcggctttc gagtccgcgg tcgcgagcag
     1141 gtctttaagc tgcgcgtcgt tgacgacgta cttgaggtgg cggcacttcg gctggatgcc
     1201 gtcgaagctt ttcgccagcg cggaggagaa gaagagcgcc cgggcgcgac tgtcgttgag
     1261 ctggtattcg tagtccgccg gcgtgagcag cgtgttcagc gccaccggca cgacgccggc
     1321 cttgatcgcg ccgaggaaga gcgccaccca atccgccgag tcgagcatcg cgatggcgac
     1381 acgctgctcg cgcttgatgc cgaggccgcg cagcgcgctc gtggcacggt cgacgcgctc
     1441 ggcgagctcg gcgtaggtcg tgctgcccga atcgtcgatg taggcgatct tgccgccgcg
     1501 gcccgccgcg aggtggcgct cgatcaggtc taccgcggcg ttgtattccc gggggatctc
     1561 cattgctcct gctcctcctc gctcgccgga gcattgttcg cggcgccagc ctcagcgtca
     1621 aggcgcaggg cctcgcggaa gaagcgctcc gccgccgggc cgagacggcg gcccttggga
     1681 tgggcgaggc tccagcgccg ctcttccggc aggccttcga cctcgagcgc gaagaggtag
     1741 acgtcgtccg cgccggccgc gagtcgcgcc agcaggtcgg cgcgctcgcc gacgtgaagc
     1801 gtggcctgca cgccgggatg gtgcgcgcag aagtcggcaa gcatgcgcgc cgcgaccgcg
     1861 cgcgcctcgg gctcggcggc gagcagcagg ttctcctttt tcggttggcg ccaggcggcg
     1921 agcctcgttt ctgcgcgcgc gaggcactgg agcagctcct cgcaagtctc gcgcagggcg
     1981 tgcccggcgc cggtgaggtg cagccgccgc ccgcgctgct cgaagagcgg cgcttccagc
     2041 gtttccgcga gcttcttcaa ctggagggaa acggtgggct gggcgaggtg cagctccgcc
     2101 gcggcgcgcg tcacgctgcc gaggcgcgcg acggcttcga aaaccagcag ctggggcagc
     2161 agcccatgtc ggaaaatcgg cgcgcagcac gagacatccc ggctcctttc ggtaaccgag
     2221 gtctggcttg cagcgctgga tcgggctgcc ggagtgaccg gggatgtttt ccggaatgac
     2281 ggccactccg cgggaagcta ctcccctgcg gaaaggccat tgtagaggaa tattggcggc
     2341 cacgtattgc gaaccgcaat acgttccatc gcatatcgtc aattttcagc cggccgcgcc
     2401 gcgcacagac tcggtgcatg ttcactcccg aattctggtc cgccctcgcc gccatcgtcg
     2461 tcatcgacct cgtgctcgcg ggcgacaacg cgatcgtcat cgcgctcgcc gcgcgcaacc
     2521 tgccgaaggc gctgcagcgg cgcgccgtgc tctggggcac gttcggcgcg gtcgcgctgc
     2581 gcgcgagcct caccgtcgcg gtgctctggc tgctggagat ccccggcctg atgctcgcgg
     2641 gcggcatcct gctcgcgtgg atcggctacc gcctgctcgc cggcagcgag aacggcggcg
     2701 agcacgaggt aaccccggcg aggagcttct ggggtgcgat gcgcaccatc gtcgtcgccg
     2761 acgccgtgat gggcctcgac aacgtgctcg cggtcgcggg cgccgcccac ggcagcgcgg
     2821 tgctagtggt cgccgggctc gtcatcagca tcccgatcgt ggtgtggggc tcgaccctga
     2881 tcctgcactg gatcgagcgc tttcccgcgc tgctgtacct cggcggcgcg gtgctcgcct
     2941 ggaccgcggc aaagatgatc gccgacgagc cgctctacca ggaatggctc gacgagcggg
     3001 ccggcatcaa gtcgcttctg ttcatcgtga tcaccggcgg cgtgctcggc ctcgcctggc
     3061 tgcgcaaccg cagggcaaca aaggaggtct tgcaatgaaa gtgctcgttc ccgtagacgc
     3121 ttccgaggcg gcgctcgcgc cgatcggcta cctcgaaacg ctggcgcgct ccggcgttcc
     3181 gctcgaagtg ctggtgatga acgtgcagcc gcgcttccac cgccacgtcg cgcgcttcac
     3241 cagccgcgcc gcgcgcgacg gcgtgcgcgc cgagcgcagc cgcgccgcga tggcgcgcgc
     3301 gatcgaggcg ctgtcgctgc gcaacatcgc gttccgcgca ttcgccgaag tcgggctgcc
     3361 ggcgccgcgc atcgccgcag tggccgagcg cgagcaggtc gacgaggtga tgatgggcgt
     3421 cggccgccat ccggcctggc tgcgctgggt gaacccgtcg atcgcgcagg gcgtgatgga
     3481 gcgcaccgac attcccgtca ccgtgctggc gcgcggccgg gccggcgcgc tggagcgcta
     3541 cgcccttccc gccggcgtcg ccggcctcgc cgcgctgctg ctcgcgggag aatagccatg
     3601 cgcgccctga taccggttct cgattcggtg aacgcgctgc cggcggtgcg ttacgtgatc
     3661 cgcgagtccc tgagcggcga acgccacgac gtccggctcc tggacgtgcg ctcgccgctc
     3721 ggccatgcgc tggaaggctg gctgaccgga gacgccctgc agcccgcctg cgagctgctc
     3781 gacaggttcc gcgtccggca cacggtgcag cgcatcgcgg cgcaagacag ggccgacgcg
     3841 atcgtcgctg ccgccctaag cgaggggccc gacgcgatcg tcctgggcac cgcgcgttac
     3901 cgctcggcca cgcggatgag cgaggacggc gtgatccaga agctgctcga ggtcgcaccg
     3961 ttgccggtcc tgctggtgac cggaagggaa gtcagccggc tcgagcgcta cggcatcgcc
     4021 gcaggcctcg gcgcgacgct cgggctgatc ttcctgggct aggccgcggc cttggatgcc
     4081 tgccagcgat cgagcttcgc gcgcgccgag ttgcgcttcg gatcggtcgc ctcggcgtgg
     4141 cccggcgttt tcgccgtgag ctcgatgacg tagccgttcg ggtcgcggaa gtagatcgag
     4201 tggatgaagc cgtggttgga gacgccgcgc gtctcgatgc cgcgcgactt gcccttggcc
     4261 atcatcggcg cgagcgcctt ctcctccact tcgagcgcga tgtgcaggtc gtagtcgtgc
     4321 tggtccttga actcgaacgg ccggtcgggc gcctcgaaga acgcgaggta ggaaccatcg
     4381 cccatctcgt agaaggtgtg gagagtgcgg gttttgcgcc cggtcatggt ctcctcgatg
     4441 tccaaggtgc aagcgagctt gaggccgaga aaatcctcgt agaaagcgcg cgtctcttcc
     4501 gagtcgcggc agcggtaggc gttgtgatgc agtttcttga tcatggcgtg cctcagggcg
     4561 tgtggaaggg ctggtccttg atcgcgtgcg cgtagccgtg gaaggcctcg ttgaccttgg
     4621 gccagctcgg cagtgccttc atggtcgcca gccagcggcg gatgttggga taggcggcgt
     4681 agtcgcagcg cagcagatcg ccgagcgtca ggatcggcgc cgcgaagtag tcggcgatgg
     4741 tcggggtgct tccgcacagg aacttcttgc tcgggccgat caggtccttg tcgaggatgc
     4801 cgagccagac gcgtg
//
LOCUS       DATBSZ010000085         5846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1652036, whole genome shotgun sequence.
ACCESSION   DATBSZ010000085 DATBSZ010000000
VERSION     DATBSZ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652036"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VIG70_05610"
     CDS             <1..1086
                     /locus_tag="VIG70_05610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8250064.1"
                     /translation="AYSVATIATGLGGIAMGHFADRMPVRRVALFGALVPGLAFFFLS
                     GLQTTSQLYALHALMGLVGIGAIMAPLNSLSSQWLARNPGLAIGIVSAGGAAGQGLVP
                     YFARHLILTEGWRQAYVVLGVVFIGLMVPLALVLRDAPRPQIVRRPSAGGASATRLLA
                     LLSLAAALCCICMATPLVHVVTLGSDRGLPGRDAAGLLAVMMVSGMAGRIAFGRLTER
                     VGSLNTYITASAGQTLLAFMFPYAGSATQLFALSALFGFVFSGAMTSFLLCAREYAPA
                     GKTGLSIGVVMFFAWVGMAVGAWQGGLFYDLCGNYFPSFANASLGGVANLLVLALLVL
                     YTREKGVRPDRAHAPAAPRFAPRSAGG"
     gene            complement(1022..1906)
                     /gene="htpX"
                     /locus_tag="VIG70_05615"
     CDS             complement(1022..1906)
                     /gene="htpX"
                     /locus_tag="VIG70_05615"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEY8250065.1"
                     /translation="MKRIFLFLATNIAVLVVLSVIINIFGLDQWLTAEGIDYTSLLVF
                     SAVVGFAGAIISLLISKWMAKRSTGAQVIDGSEGTTQYWLVETVRKLAEKSGIGMPEV
                     AIFEGAPNAFATGAFKNSALVAVSTGLLETMSKEEVEAVLGHEVAHVANGDMVTLTLI
                     QGVVNTFVIFLSRVVGMIVDKAIFKTERGVGPGFFIASLVAQIVLGILASMIVAWFSR
                     QREFRADRGSAELLGTPRPMVAALARLGGLQTAELPQSFKASGIAPGMSKLAGLFSSH
                     PPIEVRIAALQGRAPGQV"
     gene            complement(1946..3517)
                     /locus_tag="VIG70_05620"
     CDS             complement(1946..3517)
                     /locus_tag="VIG70_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8250066.1"
                     /translation="MVVGAGSAGCLLANRLSRDPQVRVLLLEAGGRDDWFWIDIPVGY
                     LYTIANPRTDWCYKTEPDEHLAGRSIHYARGRVLGGCSSINAMIYMRGQKEDWDHWAA
                     LGNRGWSWEEVLPLFRSLEDYEHGAVDGYGAGGEVRIEDPRVRWDIIDAWREAAAECG
                     IPPVKVFNRGDNFGCAYFQMNQKRGRRWSATNAFLRPVTHRANLAVMTGAQVLKLNFS
                     GRACTGVDFVRRNEKFSVEARKETILAAGAIGSPQLLQLSGIGSPALLQSLEIPLVHY
                     LPGVGENLQDHLQIRMQYKVKNVKTLNGIANSLWGKAAMALEYFAFRTGPLTMPPSQA
                     GAFARSDPAQPTPNIEWHVQPLSLDKFGDPLHSFPAITPSVCNLRPASRGSVRIKSRD
                     ATTYPEIRLNYLKAEEDRRVAVDAMRFTRRIMSAQALAKYQPEEYRPGSRIVTDEDLA
                     KAAGELGTTIFHPVGTCKMGNDPMAVVDDELRVHGTERLRVIDASIFPRITAGNTNAP
                     TYVIAEKGARAILKL"
     gene            3579..4325
                     /locus_tag="VIG70_05625"
     CDS             3579..4325
                     /locus_tag="VIG70_05625"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEY8250067.1"
                     /translation="MDEKISGALLAVRAAHFDARGAAGDYAALAASAERARLAECLAE
                     LEGFDTRRVRIPAQMAFWINVFNAGVLRDAAELQAASGAKAVEAFFERPRLKVGAHRY
                     SLDDIEHGLLRGNVPKQGRKRTPMERGDPRLEYMPIVFDERMHFGLHSACRSSPPLRV
                     FEEGRLDRQLEEAAVDYVRQGARVENRGAVIVLPRLFHWFAEDFGGASGIVEFVVARI
                     DDEATVEMIDRRLGRVKLQYAPFDWTLNRK"
     gene            4467..5021
                     /locus_tag="VIG70_05630"
     CDS             4467..5021
                     /locus_tag="VIG70_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY8250068.1"
                     /translation="MNGTRRVFFGNAAKLSAAAVALLAGNHALAQGSGSMADVNILNV
                     ALGLEWEGINAYTLGAQSGLLQKPVLDVAVRFQADHKEHADTLAGAIRKMGGKPVEPK
                     ALGDYAKALKADTLKSQADVLDLAARLELGATNAYIGVIPSFKNPALAQVAARLAADE
                     VMHWTILNNALGRALPAGAMPFGA"
     gene            5025..5396
                     /locus_tag="VIG70_05635"
     CDS             5025..5396
                     /locus_tag="VIG70_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8250069.1"
                     /translation="MFSLKGVFACAALSAAQAFSSQALAADPKRGKTLYESRCIGCHS
                     IDENRAGPAHRGVFGRRAGIVKGFDYSPALKSSRLTWNEKTLERWLTDPEAAIPGQKM
                     GFPVSEAADRADIIEYLRTAK"
     gene            5430..>5846
                     /locus_tag="VIG70_05640"
     CDS             5430..>5846
                     /locus_tag="VIG70_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8250070.1"
                     /translation="MGGTKIEALALDGGGREVFRKRVPTPRGDYAATIQAVRLLVDEA
                     GPGSVGVGIPGAMSQVTGLVKNANSTWLIGKPLKEDLEKALARPVRLENDANCFALSE
                     AVDGAGKGAGVVFGVILGTGVGGGVVVDGKVLVGANA"
BASE COUNT          897 a         1952 c         1991 g         1006 t
ORIGIN      
        1 gcgtattcgg tcgccaccat cgcgaccggc ctcggcggga tcgcgatggg gcatttcgcc
       61 gaccgcatgc cggtgcgccg cgtggcgctc ttcggcgccc tggttcccgg cctcgcgttc
      121 tttttcctgt ccgggctgca gaccacgtcc cagctctacg cgcttcatgc actgatgggc
      181 ctggtcggca tcggcgcgat catggcgccg ctcaacagcc tgtcgagcca gtggctcgcg
      241 cgaaacccgg ggctcgccat tggcatcgtt tccgcgggcg gcgcggcggg gcaggggctg
      301 gttccctatt tcgcccgcca cctgatcctc accgaaggct ggcgccaggc ctacgtcgtc
      361 ctcggcgtgg tgttcatcgg cctgatggtc ccgcttgcgc tcgtccttcg cgacgcgccg
      421 cgcccgcaga tcgtccggcg cccgagcgcc gggggagcgt cggcgacacg cctgctcgcg
      481 ctgctttcgc tcgctgccgc gctgtgctgc atctgcatgg cgacgcctct cgtgcatgtc
      541 gtcacgctcg gctcggatcg cgggctcccg ggccgcgacg ctgcgggctt gctcgcggtc
      601 atgatggtgt ccgggatggc ggggcggatc gcgttcgggc gcctgaccga gcgcgtcggc
      661 agcctcaata cctacatcac ggcctccgcc gggcagacgc tgctcgcgtt catgtttccc
      721 tacgcgggca gcgccacgca gctcttcgcg ctgtccgcgc tcttcggctt cgttttcagc
      781 ggcgccatga cctcgttcct gctttgcgcg cgcgagtacg cgcccgcggg caagaccggg
      841 ctgtcgatcg gcgtggtgat gttcttcgcc tgggtcggca tggccgtggg cgcctggcag
      901 ggcggcctgt tctacgacct ctgtggaaac tatttcccgt cgttcgccaa cgcctctctc
      961 ggcggcgtgg cgaatctgct ggtcctcgct ctgctggtcc tctacacccg cgaaaaaggg
     1021 gtcagacctg accgggcgca cgcccctgca gcgccgcgat tcgcacctcg atcggcgggt
     1081 ggctagagaa caggccggcc agcttcgaca ttcccggcgc gatgcccgac gccttgaacg
     1141 actgcgggag ctccgcggtc tgcaggccgc ccaggcgcgc gagcgcggcc accatcggcc
     1201 gcggcgtgcc gagcagctcg gccgagccgc ggtcggcgcg gaactcgcgc tggcgcgaga
     1261 accaggcgac gatcatggag gcgaggatgc cgagcacgat ctgcgcgacc agcgaggcga
     1321 tgaagaagcc cggcccgacg ccgcgctcgg tcttgaagat cgccttgtcc acgatcattc
     1381 ccacgacgcg cgagaggaag atcacgaagg tgttgaccac gccctgaatc agggtgagcg
     1441 tcaccatgtc gccattggcg acgtgcgcca cctcgtggcc gagcaccgcc tcgacctctt
     1501 ccttgctcat cgtttccagc aggccggtgg agaccgcgac cagcgccgag ttcttgaacg
     1561 cgccggtggc gaaggcgttc ggcgcacctt cgaagatcgc cacttccggc atgccgatgc
     1621 ccgacttctc ggcgagcttg cgcaccgtct cgaccagcca gtactgggtg gtgccttcgg
     1681 agccgtcgat cacctgcgcg ccggtgctcc tcttggccat ccacttggag atgaggagcg
     1741 agatgatcgc accggcgaag ccgaccaccg ccgagaacac cagcagcgac gtgtagtcga
     1801 tgccctcggc cgtcagccac tggtcgagcc cgaagatgtt gatgatgacc gagaggacca
     1861 ccagcaccgc gatgttggtg gcgaggaaca ggaagatccg cttcattttt tctcctttat
     1921 cgggctctta gagtggggac ggattctaaa gtttcaagat cgcccgcgca cccttttcgg
     1981 caatcacata ggtcggcgcg ttggtgttgc cggcagtgat gcgcgggaag atcgaggcgt
     2041 cgatgacgcg cagccgctcg gtgccgtgca cgcgcagctc gtcgtcgacc accgccattg
     2101 ggtcgttccc catcttgcag gtgccgaccg ggtggaagat ggtcgtgccc agctctccgg
     2161 ccgctttcgc aaggtcttcg tcagtcacga ttcgggatcc aggccggtat tcctcgggct
     2221 ggtacttcgc caacgcctgt gcggacatga tgcgacgcgt aaaccgcatc gcatccaccg
     2281 caaccctgcg atcttcctct gctttcaaat aatttagcct gatctcgggg tacgtcgtcg
     2341 cgtcccgcga cttgatgcgc accgagccgc gcgaggccgg ccgcaggttg cacacgctcg
     2401 gcgtgatcgc cgggaagctg tgcagcgggt caccgaactt gtcgagcgac agcggctgca
     2461 cgtgccactc gatgttcggc gtcggctgcg cgggatcgct gcgcgcgaag gcgcccgcct
     2521 gcgacggcgg catggtgagc ggcccggtgc gaaacgcgaa atactcgagc gccatcgccg
     2581 ccttgcccca gaggctgttg gcgatgccgt tcagcgtctt cacgttcttc accttgtact
     2641 gcatgcggat ctgcaggtgg tcctgcaggt tctcgcccac gccgggaagg tagtgcacca
     2701 ggggaatttc caggctctgc aatagcgccg gcgagccgat gccggacaac tggagcaatt
     2761 gcggcgaccc gatcgcgccg gcggcgagga tcgtctcttt cctcgcttcg accgagaatt
     2821 tctcgttgcg gcggacgaaa tcgacgccgg tgcaggcccg gccgctgaaa ttcagcttga
     2881 ggacctgggc gccggtcatg acggcgaggt tggcgcggtg cgtgaccggg cgcaggaacg
     2941 cgttggtcgc gctccagcgc cggccgcgct tctggttcat ctggaagtag gcgcagccga
     3001 agttgtcgcc gcggttgaag accttgaccg gcgggatgcc gcattcggcc gccgcctcgc
     3061 gccaggcgtc gatgatgtcc cagcgcacgc gcgggtcctc gatgcgcacc tcgccgccgg
     3121 cgccgtaccc gtcgacggcg ccgtgctcat agtcctccag ggaccgaaac aagggcagga
     3181 cttcctccca cgaccagccc cggttgccga gcgccgccca gtggtcccaa tcctccttct
     3241 ggccgcgcat gtagatcatg gcgttgatcg atgagcagcc gccgagcacg cgtccgcggg
     3301 cgtagtggat cgagcgcccg gcgaggtgct cgtcgggctc ggtcttgtag caccagtcgg
     3361 tgcgcgggtt ggcgatggtg tagaggtagc cgaccgggat gtcgatccag aaccagtcgt
     3421 cccgcccccc ggcctcgagg agcaggaccc ggacttgcgg gtcgcgcgaa agccggttcg
     3481 ccaggaggca gccggcgctg cccgccccca cgaccacgta atcgaatgcg ccgaaattct
     3541 gcaggttcgc ctttcccggc ggtattgtaa tgagcgggat ggacgagaaa atctcgggag
     3601 cgctcctggc ggtgcgggcc gcccacttcg acgcgcgcgg cgcggcgggc gactacgccg
     3661 cgcttgccgc ctcggcagag cgtgcgcggc ttgccgagtg cctcgccgag ctcgagggct
     3721 tcgacacccg gcgggtgcgc attcccgcgc agatggcctt ctggatcaat gtgttcaacg
     3781 ccggcgtgct gcgcgacgcg gccgagctgc aggccgcctc gggcgcaaag gcggtcgagg
     3841 cgttcttcga gcgcccgcgc ctcaaggtcg gcgcccaccg ctattcgctc gacgacatcg
     3901 agcacgggct gctgcgcggc aacgtgccca agcagggccg caagcgcacg cccatggagc
     3961 gcggcgaccc gcgcctcgag tacatgccga tcgtgttcga cgagcgcatg cacttcgggc
     4021 tgcattcggc ctgccgctcg tcgcctccgc tgcgggtgtt cgaggagggc cggctcgatc
     4081 gccagctcga ggaagccgcg gtcgactacg tgcgccaggg cgcgcgcgtc gaaaaccggg
     4141 gcgcggtgat cgtgctgccc cggctgttcc actggttcgc cgaggatttc ggcggcgcga
     4201 gcggcatcgt cgaattcgtc gtcgcgcgca tcgacgacga agccacggtt gagatgatcg
     4261 accggcggct cggaagggta aagctgcagt acgccccgtt cgactggacg ctcaatcgaa
     4321 agtgaagtcg cgggaaaaat gaatacgaat ccgccgccgc gccggatgca ctgagtttcg
     4381 cctttccttc cgcccgaatc cgcgccgcgc ctgccgccgt aaatggcggc gtgcgcgctt
     4441 gcgcatacga ccaacaagga gcggaaatga acggaaccag acgtgtcttt tttggcaacg
     4501 cggcgaagct gtccgcggcc gccgttgcgc tgctcgccgg caaccacgcc ctcgcgcagg
     4561 gctcgggcag catggcggac gtcaacatcc tcaacgtcgc gctcgggctg gagtgggaag
     4621 gcatcaacgc ctataccctc ggcgcgcaaa gcggcctgct gcagaagccg gtgctcgacg
     4681 tggcggtgcg cttccaggcc gaccacaagg agcacgccga caccctcgcg ggcgcgatcc
     4741 gcaagatggg cggcaagccg gtcgagccga aggcgctggg cgactacgcc aaggcgctca
     4801 aggcggacac gctgaagagc caggcggacg tgctggatct cgccgcgcgg ctcgagctcg
     4861 gcgcgaccaa cgcctacatc ggcgtgatcc cctcgttcaa gaacccggcg ctcgcccagg
     4921 tcgcggcgcg cctcgcggcc gacgaggtca tgcactggac gatcctcaac aacgccctcg
     4981 gccgggcgct gcccgccggc gcgatgcctt tcggcgcttg ataggtgttc tcccttaaag
     5041 gtgtctttgc ctgcgcggct ctctccgccg cgcaggcgtt ttcatcacag gcgctggcag
     5101 ccgaccccaa gcgcggaaag acgctttacg agagccgctg catcggctgc cattcgatcg
     5161 acgagaaccg cgccggccct gcgcaccgcg gcgtctttgg ccgcagggcg ggcatcgtga
     5221 aggggttcga ctattcgccc gcgttgaaat cttcccgttt gacctggaac gagaagaccc
     5281 tcgagcgctg gctgaccgat ccggaggccg cgatcccggg acagaagatg ggcttcccgg
     5341 tgtccgaggc ggccgaccgc gcggatatca tcgagtacct gcgtacggcg aaatgaggcg
     5401 acggtatttc ggtcaggatc ggcatcgacc tgggcggcac gaagatcgaa gcgctggcgc
     5461 tcgatggcgg cgggcgtgag gttttccgca agcgcgttcc tacgccgcgc ggcgactacg
     5521 cggccacgat ccaggcagtt cgattgttgg tcgatgaggc gggccccggt tctgttggtg
     5581 tcggcatacc cggagcaatg tcgcaggtga ccggactggt gaagaacgca aactcgacct
     5641 ggctcatcgg caagccgctc aaggaggatc tcgagaaggc gctggcaagg ccggtcaggc
     5701 tggaaaacga cgccaactgc tttgccttgt cggaagcggt ggacggagcg gggaagggcg
     5761 cgggcgtggt gttcggcgtg atcctcggca ccggggtcgg cggcggggtc gtggtcgacg
     5821 gcaaggtgct ggtcggggcg aacgcc
//
LOCUS       DATBSZ010000086         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1447655, whole genome shotgun sequence.
ACCESSION   DATBSZ010000086 DATBSZ010000000
VERSION     DATBSZ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447655"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1039
                     /locus_tag="VIG70_05645"
     CDS             47..1039
                     /locus_tag="VIG70_05645"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY8250071.1"
                     /translation="MSDDVSRRTFVRTAGVSTAGLALANLMLTSAEARHGRDRDRTRD
                     FIRDVKQARLFDLSFTWDENSPIAGVNPPYAMELAATHANTLGTFGDGGKLSFTSEIM
                     QWSGQHGAPSIDAIGHIGRNGKLFGGVDAVKATSDPRGLGRSGVGAHLDIAHYPKEML
                     VNRAVLLDVARFINRNKREALAAGFDITGDMLHDTARHQGVRIERGDTVLIRTGWGKY
                     FKTNPDFYKGDAAAGVGVSGARFLVREKARVVGNDTLTFEVRPPIAGTPGGPDFEVFP
                     VHMLLIPDNGINIIENYFLEEIAAAEEYEFILVVPPIKIRGGTGSALRSFALVP"
     gene            1041..>2125
                     /locus_tag="VIG70_05650"
     CDS             1041..>2125
                     /locus_tag="VIG70_05650"
                     /inference="COORDINATES: protein motif:HMM:NF022073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HEY8250072.1"
                     /translation="MKPLWIGAALSLAAFSAPLVADETPIVRSSLKVTVYDGVTDDLL
                     SAGLNQAGLISAVAPGFADPNAPTPAELRRRAFHGNYRGIVDTVPGGGIGLLWGPGSA
                     GSPTFPAPVVPGLIPGTEYKAYLRLPEHNPHINNVPAAVQIPRHFNPEKPCIVAALPS
                     GSRSLYGGMAIAEWALFKGCAVALPGKGTDTGFHLLDAVPYAVDDLDGVAGSPGTLGN
                     RAQFALRDSRKLEAYATANPHRIAVKHAHSQINPERLWGEFALQGIEFAFWALNDHFR
                     GKPHFSRKNTLVIAAGASNGGGMALRALEADDKGLIDGLVVTEPNVGPRDGNFTIKFG
                     ADAPFDPAGRTIYDSITLFSVYAACAA"
BASE COUNT          346 a          772 c          699 g          308 t
ORIGIN      
        1 ggcctttagg ctacactccc gccacccaac ctccgaggag gacaccatga gcgacgacgt
       61 cagccgacgc accttcgtac gaaccgccgg tgtgtcgacg gcgggcctgg ccctggccaa
      121 cctgatgctc acctcggccg aggcccggca cgggcgggac cgcgaccgga cgcgggactt
      181 catccgcgac gtcaagcagg cacggctctt cgacctgtcg ttcacgtggg acgagaactc
      241 gccgatcgcg ggcgtcaacc cgccgtacgc gatggagctc gccgccacgc acgccaacac
      301 cctcggcacc ttcggcgacg gcggcaagct ctcgttcact tccgagatca tgcagtggag
      361 cggccagcac ggcgcgccca gcatcgacgc gatcggccac atcggccgca acgggaagct
      421 cttcggcggc gtggacgctg tgaaggccac cagcgacccg cgcggcctcg ggcgcagcgg
      481 cgtcggcgcg catctcgaca tcgcgcatta cccgaaagag atgctggtga accgggcggt
      541 gctgctcgac gtggcgcgct tcatcaaccg caacaagcgc gaggcgctgg cggccgggtt
      601 cgacatcacc ggcgacatgc tccacgacac tgcgcgccac cagggcgtgc gcatcgagcg
      661 cggcgacacg gtgctcatcc gcaccggatg gggcaagtac ttcaagacga accccgactt
      721 ctacaagggc gacgccgccg cgggcgtcgg cgtgagcggc gcgcgcttcc tggtgcgcga
      781 gaaggcgcgc gtggtcggca acgacacgct caccttcgaa gtgcgcccgc caatcgccgg
      841 cacgccgggc gggcccgatt tcgaggtgtt cccggtgcac atgctgctca tcccggacaa
      901 cggcatcaac atcatcgaga actatttcct ggaggagatc gccgcggcgg aagagtacga
      961 gttcatcctg gtcgtgccgc cgatcaagat ccgcggcggc accggctcgg cgctgcgctc
     1021 cttcgcctta gtgccctgac atgaaaccgc tctggatcgg cgcagcgttg tctctggcgg
     1081 cgttctcggc tccgcttgtc gcggacgaga cgccgatcgt ccgcagcagc ctcaaggtca
     1141 ccgtctacga cggcgtgacc gacgacctct tgtccgcggg cctcaaccag gccgggctga
     1201 tcagcgcggt ggcgcccggc ttcgccgacc cgaacgcccc gacgcccgcg gagctgcgcc
     1261 ggcgcgcgtt ccatggcaac tatcgcggca tcgtcgacac ggttccgggc ggcggcatcg
     1321 gcctgctctg gggtcccgga tctgcgggtt caccgacgtt tcccgccccg gtggttcccg
     1381 ggctgattcc agggaccgag tacaaggcct acctgcgcct gccggagcac aacccccaca
     1441 tcaacaacgt gccggcggca gtacagatcc cgcgccactt caatcccgag aagccgtgca
     1501 tcgtcgccgc gctgccctcg ggttcgcgca gcctctacgg cggcatggcg atcgccgagt
     1561 gggcgctctt caagggctgc gcggtggcgc tgccgggcaa aggcaccgac accggtttcc
     1621 acctgctcga cgccgtgccc tacgcggtgg acgacctcga cggcgtcgcc ggctcgccgg
     1681 gcacgctcgg caaccgggcg cagttcgcgc tgcgcgacag ccgcaagctc gaggcctacg
     1741 ccacggcgaa cccgcaccgc atcgccgtca agcacgcgca ctcgcagatc aaccccgagc
     1801 ggctgtgggg cgagttcgcc ctgcagggca tcgagttcgc gttctgggcg ctgaacgatc
     1861 acttccgcgg caagccgcac ttctcgcgca agaacacgct ggtgatcgcg gcgggcgcct
     1921 cgaacggcgg cggcatggcg ctgcgagcgc tcgaagccga cgacaagggc ctgatcgacg
     1981 gcctggtggt gaccgagccc aacgtcggcc cgcgcgacgg gaatttcacc atcaagttcg
     2041 gcgccgacgc gccgttcgac ccggcggggc gcacgattta cgattcgatc accctcttca
     2101 gcgtgtacgc ggcgtgcgca gcgca
//
LOCUS       DATBSZ010000087        19741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_422656, whole genome shotgun sequence.
ACCESSION   DATBSZ010000087 DATBSZ010000000
VERSION     DATBSZ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19741
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422656"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..388
                     /locus_tag="VIG70_05655"
     CDS             230..388
                     /locus_tag="VIG70_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250073.1"
                     /translation="MRSDRFRFGLRPDYRSGIQAFTAGVDKVDLRAVVGAQRFESVLA
                     HAEDFLLA"
     gene            complement(440..1522)
                     /gene="amrS"
                     /locus_tag="VIG70_05660"
     CDS             complement(440..1522)
                     /gene="amrS"
                     /locus_tag="VIG70_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEY8250074.1"
                     /translation="MERVNESYPGRWWHALEDGRVQCDLCPRDCKLHEGQRGLCFVRK
                     MEQGRMVLTTYGRSSGFCVDPVEKKPLNHFYPGTSILSFGTAGCNLACKFCQNWDISK
                     SKEMDRLQDQASPEKIAEAAVRMGCKSVAFTYNDPVIFAEYAMDIADACHDKGVQAVA
                     VTAGYIHAKPRREFYAKMDAANVDLKAFTDGFYYKICGGRLQPVLDTLVYLVKETEVW
                     TEITTLLIPGKNDSDEELKEECKWIRANLGPNVPLHFTAFHPDWKMTDVPRTPAATLT
                     RAREIAQLAGLNYVYTGNVHDEAGGSTYCPQCEQAVVSRDWYDIRGYELDDRGTCKYC
                     RTQIPGRFQKFSKPFGPRRIPVRLAA"
     gene            complement(1510..2868)
                     /gene="amrB"
                     /locus_tag="VIG70_05665"
     CDS             complement(1510..2868)
                     /gene="amrB"
                     /locus_tag="VIG70_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEY8250075.1"
                     /translation="MSSIRPAAVAGMFYPGDARALAAEVDDLLGGVGVPAPRLGYPKA
                     LVVPHAGYIYSGPVAARAYDELAAARGSVRRVVLLGPTHRVAVKGLAVPSAAAFATPL
                     GTVRIDAEALREVGDLPQVVASDAAHALEHSLEVQLPFLQKVLGEFALAPFAVGRASV
                     EEVAEVIERLWGGPETLVVVSTDMSHYHSYEQARAIDGATIDRIAAFATDIDHEEACG
                     ATPLNGLLFSSKRKNLSLKLLAACNSGDTAGGKGRVVGYSSFGLYEGGEVSLDDAGRK
                     LLSLARGAIENSLLSTASPAPDSPWLKQTGATFVTLTKDDALRGCIGSLAPSRPLGDD
                     VAQNALGAAFRDPRFPALTAAEWPQCKVEVSLLSTPKPLRFADEADLLEQIEAGEDGL
                     IIEALIDGVVKRGTFLPQVWEDLPEKRAFLAQLLKKAGLPADTRLTRCKVARYRVVKW
                     KE"
     gene            complement(2865..3341)
                     /locus_tag="VIG70_05670"
     CDS             complement(2865..3341)
                     /locus_tag="VIG70_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY8250076.1"
                     /translation="MDELAKRLGQRLKAQGEKLVTAESCTGGWVAQVVTSVAGSSDWF
                     ERGFVTYSNDAKQELLGVRPSTLETHGAVSEQTAREMAQGALARSKGSIALAVTGVAG
                     PGGGSREKPVGMVCFAWAYGKDLESETRRFPGDRESVRRQSVIHALEGVLKRIIAA"
     gene            complement(3341..3826)
                     /locus_tag="VIG70_05675"
     CDS             complement(3341..3826)
                     /locus_tag="VIG70_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEY8250077.1"
                     /translation="MIVFRPRPGFVFAHPAHAIAFGFGAGLAPAAPGTAGTALGWAIG
                     WGLGTLPPTAMLATAFAFFVIGLWACEVTGRNLGVADHGGMVWDEVAAFLLVLAIVPR
                     ELAWQAAAFVAFRFFDIVKPPPIRHLERRYGGGFGVMFDDVVAAAYTLLLLAAVKRLL
                     F"
     gene            complement(3823..4797)
                     /gene="thiL"
                     /locus_tag="VIG70_05680"
     CDS             complement(3823..4797)
                     /gene="thiL"
                     /locus_tag="VIG70_05680"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY8250078.1"
                     /translation="MALLAVDPGARGVNEFELIQRYFARPAKRALLGVGDDCALLRPD
                     AGLDLAVTTDMLIEGRHFRPGAEPRALGHKALAVNLSDLAAMGATPRWATLAIALPAA
                     EPAWLAEFSAGFYALAERYGIELVGGDTTASEARAICITAIGEVPPGMALERAGAQPG
                     DDVWVSGELGGAALGLAHPEIAEAAKRLHLPEPRVELGERLRRLAHSAIDVSDGLAGD
                     LGHIAERSGVLAIIEYDLVPRAKAFKSLKAPELEKDCVLSGGDDYELLFTAPRGRSAE
                     LGALAAELKLPLTRIGRIEAGEARITVLAADGKPIAHRGGFDHFEKRK"
     gene            complement(4758..5603)
                     /locus_tag="VIG70_05685"
     CDS             complement(4758..5603)
                     /locus_tag="VIG70_05685"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY8250079.1"
                     /translation="MDDPRSLAILAASGVGMPHDLGAALRHLQQAAEHGCRSSQAELA
                     ALVGNWRLVGEIAAGKTLKDGMPERLSAAVDLAAWRRLPEGEAMSAAPRIAMVRKFIS
                     APICDWLMRLGAPHLRAAEVFDVESGDPAEYVGRTNRSAELLLARSDVVLAFVRTRIA
                     ALANVAIQDLEPSQILHYEVGQEFRPHHDFFDVSLPGYAREVAERGQRALTVLIYLND
                     GYEGGHTAFPHLGRSFKGRKGDALVFWNVGEDGAPDWRTLHAGTAPTAGEKWLFSQWI
                     RVRAA"
     gene            complement(5603..6025)
                     /gene="nusB"
                     /locus_tag="VIG70_05690"
     CDS             complement(5603..6025)
                     /gene="nusB"
                     /locus_tag="VIG70_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY8250080.1"
                     /translation="MRHARRQAREVALQALYAWQLSGCDPLEEARSNEAYEKTDFLDE
                     LLRGVRSRAADLEGLIAPHLDREFARLSPVERAILYIGTFELSAHPKTPFKVVLNEAV
                     ELGKSFGASEGYRFVNGVLEKIAAQLRPEEVERSRNRA"
     gene            complement(6012..6425)
                     /gene="ribH"
                     /locus_tag="VIG70_05695"
     CDS             complement(6012..6425)
                     /gene="ribH"
                     /locus_tag="VIG70_05695"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY8250081.1"
                     /translation="MRLGIVASRFNEEICGKLLERARAEAQKQKAEFTVVQVPGALEI
                     PLALQWMAQSGRFDALVALGVVIRGDTYHFDIVANESARGVMDVMLELGVPVANGILT
                     TDDEAQAKARLDKGAEAVRVAVEMAKLRVQLHEAR"
     gene            complement(6430..7515)
                     /gene="ribBA"
                     /locus_tag="VIG70_05700"
     CDS             complement(6430..7515)
                     /gene="ribBA"
                     /locus_tag="VIG70_05700"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEY8250082.1"
                     /translation="MRRESAAISPVAEVIQAVRAGEIVILVDDEDRENEGDLVFAAEF
                     VTPEKINFLAKHGRGLVCMPITEEHATRLGLRPMVEQNRSRHGTNFTVSIEAAEGIAT
                     GISAHDRALTIRVASSPNAKSDDIVQPGHVFPLIAHPGGVLVRAGHTEACCDLARLAG
                     LTPAAVLCEIMRDDGTMARLPDLLEFAATHKLRIGTIADLIEYRSRNESLVQREQERD
                     IDTPWGSFHLVSYRDLAVGSLHLALVAGKPDARKETLVRVHEPLSILDLLDSGKGTHS
                     WGVGEALRKVQEAGCGVMVLMNCNQEEARPPKAGRGIDLRLYGIGAQILRDLGVGKMR
                     LMAAPRKMPSMAGFGLEVTSYVEQPRR"
     gene            complement(7525..7920)
                     /gene="rplS"
                     /locus_tag="VIG70_05705"
     CDS             complement(7525..7920)
                     /gene="rplS"
                     /locus_tag="VIG70_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY8250083.1"
                     /translation="MDLIRTLEQEEIKRLKKEVPDFSPGDTVIVNVNVVEGERKRSQA
                     FEGMVIAKRNRGLNSSFIVRKISSGEGVERTFQTYSPLIASIEVKRKGDVRRAKLYYL
                     RARSGKSARIRERTGGEEAGSAGGGKAEE"
     gene            complement(7992..8687)
                     /gene="trmD"
                     /locus_tag="VIG70_05710"
                     /pseudo
     CDS             complement(7992..8687)
                     /gene="trmD"
                     /locus_tag="VIG70_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
     gene            complement(8669..9127)
                     /gene="rimM"
                     /locus_tag="VIG70_05715"
     CDS             complement(8669..9127)
                     /gene="rimM"
                     /locus_tag="VIG70_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY8250084.1"
                     /translation="MGRVAGSYGVRGWIKVEAPQQALSRCTRWSIGGAEFPVEQVKEH
                     SGTLLAKLAGLESREAALKLKGSTVYVRRESLPAPEAGHYYLADLVGLEVVNEKGVVL
                     GVVKRWLFNGAQDVMEVGGDRTRLIPWIESVVRQVDLGKRQVQVEWGADW"
     gene            complement(9141..9392)
                     /gene="rpsP"
                     /locus_tag="VIG70_05720"
     CDS             complement(9141..9392)
                     /gene="rpsP"
                     /locus_tag="VIG70_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY8250085.1"
                     /translation="MVVIRLRRGGANKRPFFHVVVADSRRAASKKFIERLGFYDPKAP
                     AGRESLRVNMERVKHWTGKGAQLSGTAARLVKQFAKKAA"
     gene            9518..9967
                     /locus_tag="VIG70_05725"
     CDS             9518..9967
                     /locus_tag="VIG70_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8250086.1"
                     /translation="MRLGLIGLASFALTACAPDSANVEAEVQQLVRSYITSTDIAASV
                     NLLDSAPSVTSITGQGRIVRGRDAIKEEASKQIATLPQLKVTAGEIQVTRIGTTHALA
                     IAPFGIGPSSASQTPTTQGAATLLLAKRDSAWKIVHEHFSYAAALKP"
     gene            complement(9964..10908)
                     /locus_tag="VIG70_05730"
     CDS             complement(9964..10908)
                     /locus_tag="VIG70_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250087.1"
                     /translation="MAEDSGRTRVCSVLFLDIVDYSKRDVGEQVRLKQRFNLVMASAL
                     GHVEPDERVVVDTGDGAAITFLGDPERALYVALEVFDNVGELPVRMGINLGAVSLMKD
                     LNGLDNVIGDGINVAQRVMSFAGSGELLVSKLYYDVVSRLSDDYASMFRAEGSHKDKH
                     QRAHDVYSVSKAVRVGRKVASEQLIAKRKRRAGPDATFQPVGRTPAQVFDVGTHYLVS
                     GYSKEGVSDAVEKLTAQGAKLISPLSQVGRKWLASVTNPKLDVEATVEELGFKRVITG
                     PTRESVEVKLDDLIERGSKLVQPPELTDGVWTAVCEHV"
     gene            complement(10920..12269)
                     /gene="ffh"
                     /locus_tag="VIG70_05735"
     CDS             complement(10920..12269)
                     /gene="ffh"
                     /locus_tag="VIG70_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY8250088.1"
                     /translation="MLDTLTSRLARIVKTIRGEARLTEANIQEALREVRVALLEADVA
                     LPVVKHFTDSVRAAALGEEVVGSLTPGQALVGVVHRSLTRLMGEANVGLDLATQPPAV
                     ILMAGLQGSGKTTTCGKLARLLVNDKKKVALVSCDVYRPAAIQQLETVAGQANAEFIQ
                     SSVGEKPGDIARRALEYARTRYMDVLIVDTAGRLAIDEEMMREIAELHATLKPIETLF
                     IVDAMQGQDAVNVARAFDERLPLTGVILTKLDGDARGGAALSVRHVTGKPVKFAGVGE
                     KLDGLEPFHPERMAARILGMGDILSLVEEAHAKIDLEEAQRVAEKVKKGKGFDLEDFK
                     QQIVQMKKMGGVAALMDKLPAQLVGQIDPAKLQDEKQFKRIEGIINSMTPAERRKPEL
                     IKASRKRRIAAGAGVPVQAVNQLLNQFEQMQKMMKMMKGGNLARMMRGMKGMLPGIR"
     gene            12302..13120
                     /gene="ccsA"
                     /locus_tag="VIG70_05740"
     CDS             12302..13120
                     /gene="ccsA"
                     /locus_tag="VIG70_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY8250089.1"
                     /translation="MPDIVIHGIVSAMYVGLAWHFWNTRWRGRAPAGVQQAGLRGWER
                     AAILVPLGLHGWLLYQGVLGRELRFGFAQALSVMMFIGVALYWVESLYYNLEGLQPLV
                     LPLAALAAPLPALFAGRSIRDFSTEFKLHLILAMIAYSLFVMALLHAALMALVERRLH
                     QRSASPPGNLPPLLTVEHSLFRVIAAAFVFLTLTLATGMALTDAMFGRLLRFNHETVF
                     AILSWATFAGLLLGRYAYGWRGRTALRWTIAGFVLVLLANIGTAFVLEVVLRRT"
     gene            13120..14418
                     /locus_tag="VIG70_05745"
     CDS             13120..14418
                     /locus_tag="VIG70_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEY8250090.1"
                     /translation="MEDLTLQWMVGSLVVLLVISGFFSIAETSMMAINRYRLKHMVAQ
                     GHGGAAHVEELLRRTDKLLGAILIGNNLVNAAAATIASLIAVELFGQGKLAYAVSTLA
                     ISFLIIVFAEITPKVIGATYPERIAPALAYVVKPVQWLLQPGVWFVNLFVRPLLLLVG
                     IRPEENTQVEKLSPEEIRTLVLESSSFMPKKHFSILMNLFDVGAVTVQDIMVPRARIE
                     SIKLDDDMETIIHQIVTSYHQRLPVFRSALGEVIGVLHLKKVLAALPTRTLDKTTLEE
                     LLGEPYFVPATTPVLAQMQYFQENRERIAMVVDEYGELMGLVTLEDIIEEIIGKFTTS
                     LPSAGLALTWDPSGAATAEGTTPVREVNRALGLSLPTDGPKTLNGLIVEHLQDIPEAD
                     VSIKIGNVPMEIVHAQGRTVKTVRIFKPVEIVENTEPSEA"
     gene            14499..15722
                     /locus_tag="VIG70_05750"
     CDS             14499..15722
                     /locus_tag="VIG70_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8250091.1"
                     /translation="MATAAAALKGPKERTFTWEGKDKAGKTIRGELRAASEAAANATL
                     RRQGIVVQKVKPVRRKRGGSVSSKDISLFTRQLATMMKAGVPLLQSFDIVSKGATNPA
                     VGKLLTDIKTDVETGSSLAAAFRKFPLHFDNLYCNLVQAGEAAGILETLLDRLATYQE
                     KTLAIKSKIKAALFYPIAIVAVAFIITAVIMIFVIPAFKEVFTNFGADLPAPTLLVMA
                     ISDWFVSYWYIIFPIIGLAIYGFFESWKRSLAVQMFMDKWMLRVPVFGHLVRISTIAR
                     WTRTLSTMFAAGVPLVEALDSVGGASGNYVYAAATKQIKQEVATGTSLTVAMQNSNVF
                     PSMVIQMCSIGEETGALDGMLAKVADFFEAEVDDAVEALSSLMEPMIMVVLGTLIGGM
                     VIAMYLPIFKIGQAV"
     gene            15727..16593
                     /locus_tag="VIG70_05755"
     CDS             15727..16593
                     /locus_tag="VIG70_05755"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY8250092.1"
                     /translation="MTAAGWLVQPAVFPWAALVLGLCVGSFLNVVIHRLPKMLERGWR
                     AECAELTGQPLPPEDAYNLVVPRSGCPKCGYRIRAWQNIPVVSWLVLRGKCAGCGAKI
                     GLKYPLVELLAGIGAAYAAWRFGASAAALGATLFVWFTIALAFIDQETGLLPDDLTLP
                     LVWLGLLVNLYGAFVPLADAVIGAVAGYLALWLIYQGYKLLTGKEGMGYGDFKMNAAV
                     GAFLGWKILPLVILLSSLVGLVFGALQMFAARGRWDAGFRFHFGPYLAIAGIVAMFWG
                     EPIVRWYLQRIL"
     gene            16590..17192
                     /gene="coaE"
                     /locus_tag="VIG70_05760"
     CDS             16590..17192
                     /gene="coaE"
                     /locus_tag="VIG70_05760"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234741.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY8250093.1"
                     /translation="MTFVVGLTGGIGSGKSAAADEFAKLGVTIVDTDVISHELTRNNG
                     AAMPAIRKLFGPDAVEPNGAMNRKKVRDLVFADPQKKRSLEALLHPMIREESVRRIAA
                     ASGPYVVHVVPLLVESPDYRSRVDRVLVVDCPEETQVERVRARSGLSEPEVRAIMRGQ
                     IPRAERLAAADDVIENGGARDALRNQVAALHQKYLQFASP"
     gene            17189..17947
                     /gene="zapD"
                     /locus_tag="VIG70_05765"
     CDS             17189..17947
                     /gene="zapD"
                     /locus_tag="VIG70_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEY8250094.1"
                     /translation="MITYEYPLNERIRTLLRLEDLFERSRHFIARTEPHDHHMALLTL
                     FEIMEVAGRADLKSDLLQELERQKQVLLSFRNNPDIAEEALAHVLRDIEQASQALFSM
                     TGKIGQYLRENDWLMSIKQRTGIPGGACEFDLPSYHYWLHRPSDERTGQLAAWTGPLY
                     PLRDGTAIILRILRESGKSQALAAPQGMYQQMLGGKTAQMLRLRLDHTMPCVPEISAN
                     KYVLNIRFLAQNGEDPKARTADWDVGFELTFCNL"
     gene            17935..18117
                     /locus_tag="VIG70_05770"
     CDS             17935..18117
                     /locus_tag="VIG70_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEY8250095.1"
                     /translation="MQSVRAVPCPRCGTLSEYSEANKWRPFCSERCRLIDLGKWASDE
                     YRLPDDKKSSSKPPES"
     gene            complement(18092..18985)
                     /locus_tag="VIG70_05775"
     CDS             complement(18092..18985)
                     /locus_tag="VIG70_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HEY8250096.1"
                     /translation="MIEVAAAVIQRGDGAFLLAQRPQGKVYAGYWEFPGGKVEPGEPA
                     DRALARELHEELGIDIGPPFPWITRVFAYPHGTVRLNFFRVHEWKGEPHPREDQAIAW
                     QSAGAPMVAPMLPANAPVLASLALPTEYGITNAAHYGIADMRARLEQRLRQGLKLLQV
                     REPGLVESERRRFTEQAIGLAHRHGCKVLVKSAFPGADGIHFTASQLMRLTGKPEGLV
                     AASCHTRAELDRAMELQLDFVVLGPVKDKSPALGWQRFAELARGATIPVYAIGGLTRA
                     DLQDAWRAGAHGLAMIRGAWS"
     gene            complement(18982..>19741)
                     /locus_tag="VIG70_05780"
     CDS             complement(18982..>19741)
                     /locus_tag="VIG70_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8250097.1"
                     /translation="RWRSGGRGRPGWLQPVRHVHRIKLSDLRGIDKQIERVEQNTRQF
                     IDGRPANNVLLTGARGTGKSSIVKGLLNKYSKQGLRLIEVEKNDLIDLPTIVDQVAPR
                     PERFLLFCDDLSFHGAEEGYIALKVALDGSISTTSENLLIYATSNRRHLMPEYMAENL
                     ETKYVGDEIHPGETVEEKISLSERFGLWVTFYPFDQDEYLEIVEQWLKSFGVSSNEKS
                     KEEALQWALQRGSRSGRVAYQFARDWAGRHAKKK"
BASE COUNT         3132 a         6835 c         6464 g         3310 t
ORIGIN      
        1 gcgatgtagg cgcgcttggc gccgaagtgg tcggcgatga agccgacgat gaagagcgat
       61 gtcaggtagc cgaggtggaa cgcagcctgc accagccccg cttctttcga agaaagattc
      121 cattcggcct gcgtcaaagg caagaccgcc gagtaggcga cgaaccatgt cgcggcgaga
      181 acgcggctgg tgcacagcgc accgagccat gtcccggagc ctggagtaaa tgaggtcaga
      241 tcgtttccgc ttcggacttc ggccggatta tcgctcggga atccaggcct tcaccgcggg
      301 cgtggacaag gtcgacctgc gggcggtcgt cggcgcacag cgcttcgagt cggtgctcgc
      361 gcacgcggag gacttcctcc tcgcctaggg cattgcggct gaaaagcaac gcccggctag
      421 ccgccgggcg ttggcttttt cacgcggcaa gtctcacggg gatgcggcgc gggccgaaag
      481 gcttgctgaa cttctggaac cggccgggga tctgcgtgcg gcaatacttg caggtgccgc
      541 ggtcgtcgag ctcgtagccg cggatgtcgt accagtcgcg gctgaccacc gcctgctcgc
      601 actgcgggca gtaggtcgag ccgccggctt cgtcgtgcac gttgccggtg tacacgtagt
      661 tgagcccggc gagctgcgcg atctcacgcg cgcgcgtcag cgtcgccgcg ggcgtgcgcg
      721 gcacgtcggt catcttccag tcggggtgga atgcggtgaa atgcagcggc acgttgggcc
      781 cgaggttggc gcggatccac ttgcactcct ctttcaactc ctcgtcggaa tcgttcttgc
      841 cgggaatgag gagcgtggtg atctcggtcc acacctcggt ttccttcacc aggtagacca
      901 gcgtgtcgag caccggctgc agccgcccgc cgcagatctt gtagtagaag ccgtcggtga
      961 aggctttcag gtccacgttc gccgcgtcca tcttggcgta gaactcccgc cgcggcttgg
     1021 cgtggatgta gccggcggtg accgcgaccg cctgcacgcc cttgtcgtgg caggcgtcgg
     1081 cgatgtccat cgcgtactcg gcgaaaatca ccgggtcgtt gtaggtgaac gcgaccgact
     1141 tgcatcccat gcgcaccgcg gcctcggcga tcttctcggg actcgcctgg tcctgcagcc
     1201 ggtccatctc cttcgacttg gagatgtccc agttctggca gaacttgcac gccaggttgc
     1261 agccggcggt gccgaacgaa aggatcgagg tgccggggta gaagtggttg agcggcttct
     1321 tctccaccgg gtcgacgcag aagcccgagg agcggccgta ggtggtgagc accatccggc
     1381 cctgttccat cttgcgcacg aagcacagcc cgcgctggcc ctcgtgcagc ttgcagtccc
     1441 gcgggcacag gtcgcactgc acgcggccgt cctcaagcgc gtgccaccag cggccgggat
     1501 agctttcgtt cactctttcc atttgacgac acggtagcgc gccaccttgc agcgcgtgag
     1561 ccgcgtatcg gccggcagcc cggccttctt cagcaactgg gcgagaaacg cgcgcttctc
     1621 cggcaggtct tcccagacct gcggcagaaa ggtcccgcgc ttgacgacac catcaatgag
     1681 ggcctcgatg attagcccat cctcgccggc ctcgatctgc tcgagcaggt cggcctcgtc
     1741 ggcgaagcgc agcggcttgg gcgtcgacag cagcgacacc tcgactttgc actgcggcca
     1801 ctccgcggcc gtcagcgcgg ggaagcgcgg gtcgcgaaac gccgcgccga gcgcgttctg
     1861 cgccacgtcg tcgccgagcg ggcgcgacgg cgcaaggctg ccgatgcagc cgcgcagcgc
     1921 gtcgtccttg gtcagggtga cgaaggttgc gccggtttgc ttcaaccaag gagaatcggg
     1981 agcgggcgac gccgtgctca acagcgaatt ctcgattgcc ccgcgggcga gagacagcag
     2041 cttgcggccc gcatcgtcga gcgacacctc gccgccctcg tagagcccga aggacgaata
     2101 ccccaccacc cgacccttgc cgcccgcggt gtcgccggag ttgcacgcgg caagcagctt
     2161 cagcgacaaa ttcttccgct ttgaggaaaa caagaggcca ttcaatggcg tcgctccgca
     2221 cgcctcctca tgatcgatat cggtcgcgaa cgcggcgatg cgatcgatgg tagcgccgtc
     2281 gatcgcgcgc gcctgctcat acgagtggta gtgcgacatg tcggtcgaga cgacgaccag
     2341 cgtttccggg ccgccccaca ggcgctcgat cacttcggcg acctcttcca cgctcgcccg
     2401 accgaccgcg aacggcgcga gcgcgaactc gccgagcacc ttctgcagga acggaagctg
     2461 cacctcgagc gaatgctcga gcgcgtgcgc cgcatcgctc gcgaccacct gcggcaggtc
     2521 gccgacttcg cgcagcgcct cggcgtcgat gcgcacggtg ccgagcgggg tggcgaacgc
     2581 cgcggcgctc ggcacggcga gccccttcac cgccacgcgg tgcgtcggac cgagcaacac
     2641 cacgcggcgc acgctgccgc gcgcggcggc gagctcgtcg taggcgcgcg ccgcgaccgg
     2701 gccggaatag atgtagcccg cgtgcggcac gaccagcgcc ttgggatagc ccaggcgcgg
     2761 cgccggcacg ccgaccccgc cgagaaggtc gtccacctcg gccgcgaggg cgcgcgcgtc
     2821 tcccgggtag aacatccctg cgaccgcggc gggtctgatc gagctcacgc tgcgattatc
     2881 ctcttcaaga ccccctccag agcgtggatc accgactgcc gcctcacgct ctcgcggtca
     2941 cccgggaagc gccgcgtctc gctctcgagg tcctttccgt aagcccaggc aaagcacacc
     3001 atgccgacgg gcttctcgcg gctgccgccg ccgggaccgg cgacgccggt gaccgccagc
     3061 gcgatcgagc ccttgctgcg cgcgagcgcg ccctgcgcca tctcgcgcgc ggtctgctcg
     3121 ctcacggctc catgggtttc cagggtggac ggacggaccc cgaggagctc ctgcttggcg
     3181 tcgttcgaat aggtgacgaa gccgcgctcg aaccagtcgg acgacccggc gaccgaggtg
     3241 accacctgcg ccacccagcc gccggtgcag gattccgcgg tgaccagctt ctcgccctgc
     3301 gccttcaggc gctggccgag ccttttcgcc agctcgtcca tcagaagagc agccgcttga
     3361 cggccgcgag cagcagcaac gtgtaagccg ccgccaccac gtcgtcgaac atcacgccga
     3421 agccgccgcc atagcgccgc tcgagatggc ggatcggcgg cggcttgacg atgtcgaaga
     3481 agcggaacgc gacgaaggcg gccgcctgcc aggcgagctc gcgcggcacg atcgcgagca
     3541 cgagcaggaa cgcggccacc tcgtcccaca ccatgccgcc gtggtctgcg accccgagat
     3601 tgcggccggt gacctcgcac gcccacaggc cgatcacgaa gaatgcgaac gccgttgcca
     3661 acatcgcggt tggcgggagc gtcccaaggc cccagccgat cgcccagccg agcgcggttc
     3721 cggcggtccc cggcgcggcg ggcgcaaggc cggcgccgaa cccgaacgcg atcgcgtgcg
     3781 ccggatgcgc gaacacgaac cccgggcgcg ggcggaacac gatcatttgc gcttctcgaa
     3841 gtggtcgaag ccgccgcgat gcgcgatggg cttgccgtcg gcggcgagca cggtgatacg
     3901 cgcctcgcca gcctcgatcc gcccgatgcg cgtgaggggc agcttgagct cggcggcgag
     3961 cgctccgagc tcggcgctcc gcccgcgcgg cgcggtgaac agcagctcgt aatcgtctcc
     4021 cccggaaagc acgcagtcct tttcgagctc gggcgctttc aacgatttga aagccttggc
     4081 gcgcggcaca agatcgtatt cgatgatggc cagcaccccg gagcgctcgg cgatgtggcc
     4141 gagatcgccg gcgaggccgt cggacacgtc gatcgcgctg tgcgcgaggc ggcgcagccg
     4201 ctcgccgagc tcgacacgcg gctcgggaag gtgcaggcgc ttcgccgcct cggcgatttc
     4261 ggggtgcgcg aggccgagcg cggcgccgcc gagctcgccc gacacccaga cgtcgtcgcc
     4321 gggctgcgcg ccggcgcgct cgagcgccat tcccggcggc acttcgccga tcgcggtgat
     4381 gcaaatcgcg cgtgcttcgc ttgctgtggt atcgccgccg acgagctcga tgccgtagcg
     4441 ctcggcgagc gcgtagaagc cggcggagaa ctcggccagc cacgcgggct cggcggccgg
     4501 cagcgcgatc gctagggtcg cccagcgcgg cgtcgccccc atcgcggcga ggtccgagag
     4561 gttgaccgcg agcgccttgt gcccgagcgc gcgcggctcg gcgccgggcc ggaagtgccg
     4621 cccctcgatc agcatgtcgg tggtgaccgc gaggtcgagc ccggcatcgg gccgcagcag
     4681 cgcgcagtcg tcgcccacgc ccagcaacgc gcgctttgcc ggccgcgcga agtagcgctg
     4741 gatgagctcg aattcgttca cgccgcgcgc acccggatcc actgcgagaa gagccatttc
     4801 tcgcccgcgg tcggcgcggt tccggcgtgc agcgtgcgcc agtcgggcgc gccgtcctcg
     4861 cccacgttcc agaagacgag ggcatcgccc ttgcgtccct tgaagctgcg gccaaggtgg
     4921 gggaacgcgg tgtggccgcc ttcgtagccg tcgttcaggt agatcagcac cgtgagcgcg
     4981 cgctggccgc gctccgccac ctcgcgagcg tagcccggca ggctgacgtc gaagaaatca
     5041 tggtgcgggc ggaattcctg gccgacctcg tagtgcagga tctggctcgg ctccaggtcc
     5101 tgtatcgcca cgttcgccag cgccgcaatg cgcgtgcgca cgaaagcgag caccacgtcg
     5161 ctgcgcgcga gcagcagctc cgcgctgcgg ttggtccggc cgacgtactc ggcggggtcc
     5221 ccgctctcga cgtcgaacac ctccgccgct cgcagatgcg gcgcaccgag ccgcatcagc
     5281 cagtcgcaga tcggagcgga aatgaacttg cggaccatcg cgatgcgcgg cgccgcgctc
     5341 attgcctcgc cctcgggcag gcgccgccac gcggcgaggt cgacggcggc actcagccgc
     5401 tcgggcatcc cgtccttgag ggtcttgccc gcggcaatct cgcccaccag gcgccagttg
     5461 cccacgagcg cggcaagctc ggcctgcgac gagcggcagc cgtgctccgc ggcctgctgc
     5521 aggtggcgca acgccgcgcc gaggtcgtgc ggcatgccga cccccgaggc ggcgaggatt
     5581 gcgagagacc gcgggtcgtc catcaggcgc ggttgcgcga gcgttccacc tcttcgggcc
     5641 gcagctgggc ggcgatcttc tcgagcaccc cgttgacgaa gcggtagccc tccgaggcgc
     5701 cgaagctctt gccgagctcg accgcctcgt tcagcaccac cttgaacggc gtcttcggat
     5761 gcgcggaaag ctcgaacgtc ccgatgtaca ggatggcgcg ctcgaccggc gagagccggg
     5821 cgaactcgcg gtcgaggtgc ggcgcgatca gcccctcgag gtcggcggcg cggctgcgca
     5881 ccccgcgcag caactcgtcc aggaaatcgg tcttctcgta ggcttcgttc gagcgcgctt
     5941 cctcgagcgg gtcgcagccg gagagctgcc aggcgtagag cgcctgcaac gccacctcgc
     6001 gcgcctggcg cctagcgtgc ctcatggagc tgcaccctca gcttggccat ttccacggcc
     6061 acgcgcaccg cctccgcgcc cttgtcgagg cgcgccttgg cctgcgcctc gtcgtcggtg
     6121 gtcaggatgc cgttcgccac cggcacgccg agctcgagca tcacgtccat cacgccgcgc
     6181 gccgactcgt tcgccacgat gtcgaagtga taggtgtcgc cgcggatcac gacgccgagc
     6241 gcgaccagcg catcgaagcg acccgactgc gccatccact gcagcgcgag cggaatctcg
     6301 agggcaccgg gcacctgcac caccgtgaat tcggccttct gcttctgcgc ctcggcgcgc
     6361 gcgcgctcga ggagcttgcc gcagatctct tcgttgaacc ggctcgcgac gatgccgagg
     6421 cgcatcgtgc taccgccggg gctgctctac gtagcttgtc acctcgaggc cgaagccggc
     6481 catgctcggc atcttgcgcg gcgcggccat cagccgcatc ttgccgacgc cgaggtcgcg
     6541 caggatctgc gcgccgatgc catacaggcg caaatcgatg ccgcgtcccg ccttgggcgg
     6601 gcgcgcttcc tcctggttgc agttcatgag caccatcacg ccgcaaccgg cttcctggac
     6661 cttccgcagg gcttccccga cgccccagga atgcgtgcct tttccggaat ccagcagatc
     6721 gaggatggag agcggctcgt gcacccgcac cagcgtctct tttctcgcgt ccggcttgcc
     6781 ggcgacgagc gccaggtgga gggaaccgac cgccaggtca cggtacgaca caagatggaa
     6841 actcccccag ggagtatcga tgtcgcgctc ctgctcgcgc tgcaccagcg actcgttgcg
     6901 gctgcggtac tcgatcaggt cggcgatggt gccgatcctg agcttgtgcg tggccgcgaa
     6961 ctcgagcagg tccggcagcc gcgccatcgt gccgtcgtcg cgcatgatct cgcacagcac
     7021 cgcggccggc gtaaggccgg cgaggcgcgc gaggtcgcag caggcctcgg tatgcccggc
     7081 gcgcaccagc acgccgccgg gatgcgcgat caagggaaac acgtggcccg gctgcacgat
     7141 gtcgtcggat ttagcatttg gagaagaagc aacccgaatc gtcagggcac gatcgtgcgc
     7201 cgagatgccg gtggcgatgc cctccgcggc ctcgatcgaa acggtgaagt tggttccgtg
     7261 gcgcgagcgg ttctgctcga ccatgggcct gaggccgagc cgggtcgcgt gctcctcggt
     7321 gatcggcatg cagaccaggc cgcggccgtg cttggcgagg aagttgatct tctcgggggt
     7381 gacgaactcg gccgcgaaca cgaggtcgcc ttcgttctcg cggtcctcgt cgtcgaccag
     7441 gatgacgatc tcgccggcgc ggaccgcctg gatgacttcg gctacggggc tgatggcggc
     7501 agattcgcgg cgcatcagcc gattttactc ctcggctttg ccgccgcccg ccgagccggc
     7561 ttcttctcct ccggtgcgct cgcggatgcg cgccgatttg cccgagcgtg cgcgcaggta
     7621 gtagagcttg gcgcggcgca cgtcgccctt gcgcttcacc tcgatcgagg cgatcagcgg
     7681 ggagtaggtc tggaaggtgc gctcgacgcc ctcgcccgag gagatcttgc gcacgatgaa
     7741 cgaggagttg aggccgcggt tgcgcttggc gatcaccatg ccctcgaagg cctgcgagcg
     7801 cttgcgctcg ccctcgacca cgttcacgtt cacgattacc gtgtcgccgg gcgagaagtc
     7861 ggggacctcc ttcttgaggc gcttgatctc ttcctgctcg agggtcctga tgaggtccat
     7921 aactatctcc tgtgttcctg ctggaattct gccagcagct tcgattcctc tgcgctcagc
     7981 ttcttcaccg ccagcaggtc gggcctgcgc agccaggtcc ttcccagcgc ctgcttcagc
     8041 cgccagcggc ggatgttctc atggtgcccg gagagcagca cctccggcac ccgccggccc
     8101 gcctcattcg gcggccaggc ctccggccgc gtgtactgcg ggcagtcgag cagaccgtcg
     8161 gcgaacgact cttccagcgc cgacgcctcg tcgccgagcg cgcccggcag ctgccgcacc
     8221 accgcgtcca ccagcgccat cgcggcgatc tcgccacccg agagcacgaa gtcgccgatc
     8281 gatacttcct cgtcgacccg gcgctcgagc agccgctcgt ccacgccttc ataccttccg
     8341 cacagcaggg tcagagcctt ttcccgcgag agctccagca ccccgcggtg atccagcttc
     8401 ctgccctgcg gcgagagata gagcaccctt ccgcctcccg ccaacctgat cccgtccagc
     8461 gcccgctcca gcggctcggc cagcatcacc atccccggcc cgccgccgta cgggcggtcg
     8521 tccaccgtcc ggtaccggtc cgccgtgaaa tcgcgcggat tccacgtggt cagcgaatac
     8581 agccccgcgt ccagcgcccg gctggtaatg ccgcagccgg tcagcgccac gaacatctcc
     8641 gggaacagcg tcaccacgtc gaagcggatc accagtccgc gccccattcg acctggacct
     8701 ggcgcttgcc cagatccacc tgccggacca cggactcgat ccaggggatc aagcgcgtcc
     8761 tgtcgccgcc gacttccatc acgtcctgcg cgccgttgaa cagccagcgc ttgaccacgc
     8821 cgagaaccac gcccttctcg ttcacgacct ccaggccgac caggtcggcg aggtagtaat
     8881 gccccgcttc gggcgcgggc agcgactcgc gccgcacgta caccgtcgag cccttcagct
     8941 tcagcgccgc ctcgcggctc tcgagccccg cgagcttcgc cagcagcgtg cccgagtgct
     9001 ccttcacctg ctcgacggga aattccgccc cgccgatcga ccaccgcgtg cagcgcgaca
     9061 gcgcctgctg cggcgcctca accttgatcc acccgcgcac gccgtacgag ccggcgacgc
     9121 ggcccatctc tacgaagtgc tcaggcggcc ttctttgcaa attgctttac gagccgcgca
     9181 gcggtgcccg agagctgcgc gccctttcca gtccagtgct tgacgcgctc catgttcacg
     9241 cgcagcgact cgcgccccgc cggcgccttc gggtcgtaga acccgagccg ctcgatgaat
     9301 ttcttgctgg ccgcgcgccg cgaatcggcc accaccacgt gaaagaacgg gcgcttgttg
     9361 gcgccgccgc gccgcagtcg aatcaccacc atttggaaga ctccgcaaac cgcgaaaagg
     9421 cgcggattat acgggagaaa tcagggcttt gggaagcttt ggaccgctac tgcatgtttt
     9481 ccgatatggt gccggtcaac cgcctgggag ggttcaaatg agactcggct tgataggcct
     9541 cgcgagcttc gcgctgacgg cctgcgcacc ggattctgcg aatgtcgaag cggaagttca
     9601 gcagcttgtc cgcagctata tcaccagtac cgatatcgcc gccagcgtga acctgctcga
     9661 ctcggcgccg tcggtgacct cgattaccgg tcagggcaga atcgtgcgcg gccgcgacgc
     9721 aatcaaggaa gaagccagca agcagatcgc cacgctgccg cagctgaaag tcacggcagg
     9781 cgaaattcaa gtaacccgga tcggcaccac gcacgccctc gcgatcgcgc ctttcggcat
     9841 cggtccgagc tcggcatccc agacgccaac gacgcaaggc gcagcgacct tgctgctcgc
     9901 caagcgcgat tcagcctgga agatcgtgca cgaacacttt agctacgccg cagcactcaa
     9961 gccttagacg tgctcgcaga cggcggtcca tacgccgtcg gtgagctcgg gcggctggac
    10021 cagcttcgag ccgcgctcga tcaggtcatc gagcttgacc tcgaccgatt ccctggtcgg
    10081 gccggtgatc acgcgcttga agccgagctc ctcgaccgtc gcctcgacgt cgagcttcgg
    10141 gttggtgacg ctggcgagcc acttgcggcc gacctgcgag agtggagaga tgagcttggc
    10201 gccctgcgcg gtgagctttt ccaccgcgtc gctcacgcct tccttgctgt accccgacac
    10261 gagatagtgc gtccccacgt cgaacacctg ggccggcgtg cgccccaccg gctggaaggt
    10321 ggcatcgggt ccggcgcggc gcttgcgctt cgcgatcagc tgctcggagg cgaccttgcg
    10381 cccgacgcgc accgccttgc tgaccgagta gacgtcgtgg gcacgctggt gcttgtcctt
    10441 gtgcgagcct tccgcccgga acatgctcgc gtagtcgtcc gagaggcgtg aaaccacgtc
    10501 gtagtagagc ttcgagacca gcagctcgcc gctgcctgca aagctcatga cacgctgggc
    10561 gacgttgatg ccgtcgccga tgacgttgtc gagcccgttc aggtccttca tcagcgacac
    10621 cgcgccgagg ttgatgccca tgcgcaccgg cagctcgccg acgttgtcga acacttccag
    10681 cgccacgtac agcgcgcgtt ccggatcgcc gaggaacgtg atcgccgcgc cgtcgccggt
    10741 gtcgacgacc acgcgctcgt cgggctccac atgcccgagc gcgctcgcca tcaccaggtt
    10801 gaaccgctgc ttcagccgca cctgctcgcc cacgtcgcgc ttcgagtagt cgacgatgtc
    10861 gaggaaaagg acgctgcaga cccgcgtgcg gcccgagtct tcggccatgc tgcctccgtc
    10921 taacggatgc ccgggagcat gcctttcatc ccgcgcatca tgcgtgccaa attcccgcct
    10981 ttcatcattt tcatcatttt ctgcatctgc tcgaactggt tcaggagctg gttgaccgcc
    11041 tggaccggga cgccggcgcc ggcggcgatg cggcgcttgc gcgaggcctt gatcagctcg
    11101 ggcttgcggc gctcggccgg ggtcatcgag ttgatgatgc cctcgatgcg cttgaactgc
    11161 ttctcgtcct ggagcttggc ggggtcgatc tggccgacga gctgcgccgg cagcttgtcc
    11221 atgagcgcgg cgacgccccc catcttcttc atctgcacga tctgctgctt gaagtcctcg
    11281 aggtcgaagc ccttgccctt cttcaccttc tcggcgacgc gctgggcttc ctcgaggtcg
    11341 atcttggcgt gcgcctcctc gaccagcgac aggatgtcgc ccatgccgag gatgcgcgcc
    11401 gccatgcgct cgggatggaa cggctcgagg ccgtcgagct tctcgccgac gccggcgaat
    11461 ttcaccggct tgccggtgac gtgccgcacg gagagcgcgg cgccgccgcg cgcatcgccg
    11521 tcgagcttgg tcaggatcac gccggtgagc ggcaagcgct catcaaatgc ccgcgccacg
    11581 ttgaccgcgt cctggccctg catcgcatcg acgatgaaca gcgtctcgat cggcttgagc
    11641 gtcgcgtgca gctcggcgat ctcgcgcatc atctcctcgt cgatggcgag ccggcccgcg
    11701 gtgtcgacga tcaggacgtc catgtagcgc gtgcgcgcgt attcgagggc ccgtcgagcg
    11761 atgtcaccgg gtttctcacc tacggacgat tggatgaatt ccgcgtttgc ctgacccgca
    11821 accgtttcca attgctggat ggctgcagga cggtagacgt cgcaggagac cagcgcgact
    11881 ttcttcttgt cgttgaccag aagacgggcg agcttgccgc aggtcgtcgt cttgcccgag
    11941 ccctgcagtc cggccatgag gatcaccgcc ggcggctgcg tcgcgaggtc gaggcccacg
    12001 ttcgcctcgc ccatcaaccg ggtgaggctg cggtgcacga cgcccaccag cgcctggccg
    12061 ggcgtcaggc tgccgaccac ctcttccccc agcgccgccg cgcgcaccga gtcggtgaag
    12121 tgcttcacca ccggaagggc gacgtccgcc tcgagcagcg cgacgcgcac ttctcgcagc
    12181 gcctcctgga tgttcgcctc ggtcaggcgc gcttcgccgc gaatcgtctt gacgatgcgc
    12241 gcgaggcgtg aagtcagggt atccagcacg ggtgtcgagg ctcgagtgat agactgaagc
    12301 ggtgcccgac attgtaattc acggcatcgt ctcggccatg tacgtcgggc tggcctggca
    12361 cttctggaac actcgctggc gggggcgcgc gccggcgggc gtgcagcagg ccggcttgcg
    12421 gggctgggag agagcggcga ttctggtgcc gctcgggctg cacggctggc tgctgtacca
    12481 gggcgtgctc ggcagggagc tgcgcttcgg cttcgcgcag gcactgtcgg tgatgatgtt
    12541 catcggcgtc gcgctctact gggtcgagag cctgtactac aacctcgaag gcctgcagcc
    12601 gctggtgctg ccgcttgccg cgctggcggc gccgctgccg gcgctgttcg ccgggcgcag
    12661 catccgggac ttcagcaccg agttcaagct gcacctcatc ctcgccatga tcgcctatag
    12721 cctgttcgtc atggcgctgc tgcacgccgc gctgatggcg ctggtcgagc gcaggctcca
    12781 ccagcgcagc gcgagccccc ccggcaacct gccgccgctc ctcacggtcg agcattcgct
    12841 gttccgcgtc atcgcggctg cgttcgtgtt ccttacgctc acgctcgcca ccggcatggc
    12901 gctgaccgac gcgatgttcg gccgcctgct gcgcttcaac cacgagaccg tgttcgcgat
    12961 cctgtcgtgg gcgacgttcg ccggcctgct gctggggcgc tatgcgtacg gctggcgcgg
    13021 gcgcaccgcg ctgcgctgga ccatcgccgg cttcgtgctg gtgctgctcg ccaacatcgg
    13081 cacggccttc gtgctggaag tcgtcctgcg gcgcacctga tggaagacct caccctgcag
    13141 tggatggtgg gctcgctggt ggtgctgctc gtgatctcgg gctttttctc gatcgccgag
    13201 accagcatga tggcgatcaa ccgctaccgc ctgaagcaca tggtggcgca gggccacggc
    13261 ggcgcggcgc acgtcgagga gctgctgcgg cgcaccgaca agctgctcgg cgcgatcctg
    13321 atcggcaaca acctggtgaa cgcggccgcg gccacgatcg ccagcctgat cgcggtggag
    13381 ctcttcggcc agggcaagct cgcctatgcg gtcagcacgc tcgccatctc gttcctgatc
    13441 atcgtgttcg ccgagatcac gccgaaggtg attggcgcca cgtatcccga gcgcatcgcc
    13501 cccgcgctcg cctacgtggt gaagccggtg cagtggctgc tgcagcccgg cgtctggttc
    13561 gtgaacctgt tcgtgcgccc gctgctgctc ctcgtcggca tccgccccga ggagaacacg
    13621 caggtggaga agctctcgcc cgaggagatc cgcaccctgg tgctggaatc ttcgagcttc
    13681 atgccgaaga agcacttctc catcctcatg aacctgttcg acgtcggcgc ggtgacggtg
    13741 caggacatca tggtgccgcg cgccaggatc gagtcgatca agctcgacga cgacatggag
    13801 acgatcatcc accagatcgt caccagctac caccagcggc tgccggtgtt ccgcagcgcg
    13861 ctcggcgagg tgatcggcgt gctgcacctg aaaaaggtgc tggcggcgct gccgacccgc
    13921 acgctcgaca agacgaccct ggaggagctg ctcggcgagc cctacttcgt gccggccacc
    13981 acgccggtgc tggcgcagat gcagtacttc caggagaacc gggagcgcat cgcgatggtg
    14041 gtggacgagt acggcgagct gatgggcctg gtcaccctcg aggacatcat cgaggagatc
    14101 atcggcaagt tcaccacctc gctgccgagc gccgggctgg cgctcacctg ggacccgagc
    14161 ggcgcggcca ccgccgaggg cacgaccccc gtgcgcgagg tgaaccgcgc gctcgggctg
    14221 tcgcttccga ccgatggccc caagaccctc aacggcctca tcgtcgagca cctgcaggac
    14281 atccccgagg ccgacgtgtc gatcaagatc ggaaacgtgc cgatggaaat cgttcacgcg
    14341 cagggccgga cggtgaaaac ggtaaggatc tttaagcccg ttgaaattgt agaaaatacc
    14401 gagccctcag aggcttagaa ccggaacttc aggctttaca aacgcccccc cggagggcat
    14461 catccggcgc ttagagggga aggcccaggg agcacgcgat ggcaacagca gcagcggcct
    14521 taaaagggcc gaaagagcgc acgttcacgt gggaaggcaa ggacaaggcc gggaaaacga
    14581 tccgcggcga gctgcgcgcg gcgagcgagg ccgcggccaa tgcgaccctg cgccgccagg
    14641 gcatcgtggt gcagaaggtc aagccggtca ggcgcaagcg cggcggctcg gtcagctcga
    14701 aggacatctc gctcttcacg cggcagctcg ccacgatgat gaaagcgggc gtgccgctgt
    14761 tgcaatcgtt cgacatcgtc agcaagggcg cgaccaaccc ggcggtcggg aagctcctca
    14821 ccgatatcaa gaccgacgtc gagaccgggt cgtcgctcgc cgcggcgttc cgcaagttcc
    14881 cgctgcattt cgataacctg tactgcaacc tggtgcaggc cggcgaggcg gcgggaattc
    14941 tggagacgct gctcgatcgc cttgcgacct accaggaaaa aacgctcgcc atcaagtcga
    15001 agatcaaggc cgccctcttc tacccgatcg cgatcgtcgc ggtcgcgttc atcatcaccg
    15061 cggtgatcat gatcttcgtg atcccggcgt tcaaggaggt gttcacgaac ttcggcgccg
    15121 acctgcccgc gcccacgttg ctcgtgatgg ccatctcgga ctggttcgtc tcctactggt
    15181 acatcatctt cccgatcatc ggcctcgcca tctacggctt cttcgagtcc tggaagcgct
    15241 cgctggcggt gcagatgttc atggacaagt ggatgctcag ggtgccggtg ttcggccacc
    15301 tggtgcgcat ctcgaccatc gcgcgctgga cgcgcacgct gtcgaccatg ttcgccgccg
    15361 gcgtcccgct ggtcgaggcg ctcgactcgg tcggcggcgc ctcgggcaac tacgtgtatg
    15421 ccgccgccac caagcagatc aagcaggagg tggccaccgg cacttcgctc accgtggcga
    15481 tgcagaactc caacgtgttc ccgagcatgg tgatccagat gtgctcgatc ggcgaggaga
    15541 ccggcgcgct cgacggcatg ctcgccaagg tcgccgactt cttcgaggcc gaggtcgacg
    15601 acgcggtcga ggcgctctcc tccctgatgg agccgatgat catggtcgtg ctcggcaccc
    15661 tcatcggcgg catggtgatc gccatgtacc tgccgatctt caagatagga caggcggtct
    15721 agcgtaatga cggcggctgg ctggctggtt cagccggccg ttttcccgtg ggcggcattg
    15781 gtgctcgggc tgtgcgtcgg ctcgttcctc aacgtcgtca tccaccggct gccgaagatg
    15841 ctcgagcgcg gctggcgcgc cgagtgcgcc gagctcaccg gccagccgct gcctcccgag
    15901 gacgcctata acctcgtcgt gccgcgctcg ggctgcccga agtgcggcta tcgcatccgc
    15961 gcgtggcaga acatccctgt cgtgagctgg ctcgtcctgc gcggcaagtg cgccggctgc
    16021 ggcgcgaaga tcggcctcaa gtatccgctg gtcgagcttc tcgccgggat cggcgccgcg
    16081 tacgcggcct ggcgcttcgg ggcttccgct gccgcgctcg gggcgacgct gttcgtctgg
    16141 ttcaccatcg cgctcgcgtt catcgaccag gaaaccggcc tcctgcccga cgacctgacg
    16201 cttccgctgg tgtggctggg gctgctggtc aacctgtacg gcgctttcgt gccgctggcc
    16261 gacgcggtga tcggcgcggt cgcgggctac ctcgcgctgt ggctcatcta ccagggctac
    16321 aagctgctca ccggcaagga gggcatgggc tacggcgact tcaagatgaa cgccgcggtc
    16381 ggcgccttcc tcggctggaa aatcctgccg ctcgtgatcc tgctctcgtc gctcgtcggc
    16441 ctcgtgttcg gcgcgctgca gatgttcgcc gcgcgcggcc gctgggacgc gggcttccgc
    16501 ttccatttcg ggccgtacct cgcgatcgcc ggcatcgtcg cgatgttctg gggcgagccg
    16561 atcgttcgct ggtacctgca gcgcatcctg tgaccttcgt agttggatta accggcggga
    16621 tcggcagcgg caagagcgcc gccgccgacg agttcgccaa gctcggcgtg accatcgtgg
    16681 ataccgacgt aatctcgcac gagctaaccc ggaacaatgg tgcggcgatg cccgcgatcc
    16741 ggaagctttt cggcccggac gcggtcgaac ccaatggggc catgaacaga aaaaaggtac
    16801 gcgacctggt attcgccgac ccgcagaaga agcgctcgct cgaggcgctg ctgcacccga
    16861 tgatccgcga ggaatccgtg cggcgcatcg cggccgcatc ggggccgtac gtcgtgcacg
    16921 tcgtgccgct gctcgtcgaa tcgcccgact accgcagccg ggtcgaccgg gtcctcgtgg
    16981 tcgactgccc ggaggaaacc caggtcgagc gcgttcgtgc gcgcagcggc ctgtcagagc
    17041 ccgaggtgcg cgccatcatg cgcggccaga tcccgcgcgc ggagcgtctc gccgccgcgg
    17101 acgacgtgat cgagaacggc ggcgcgcgtg acgcattgcg caatcaggtc gccgcgctgc
    17161 accaaaaata cctacaattc gcaagcccgt gatcacctac gagtacccgc tcaacgaacg
    17221 gatccggacg ctgctgcggc tggaagacct gttcgagcgc tcgcgccact tcatcgcgcg
    17281 caccgagccg cacgatcacc acatggcgct gctcacgctg ttcgagatca tggaggtcgc
    17341 cggccgcgcc gatctcaagt ccgacctgct gcaggagctc gagcggcaga agcaggtgct
    17401 gctctcgttc cgcaacaacc ccgacatcgc cgaggaagcg ctggcgcacg tgctgcgcga
    17461 catcgagcag gcgtcgcagg cgctgttctc gatgaccggc aagatcgggc agtacctgcg
    17521 cgagaacgac tggctgatgt cgatcaagca gcgcaccggc atccccggcg gcgcgtgcga
    17581 gttcgacctc ccttcctacc actactggct gcaccgtccc tccgatgaac gcaccggcca
    17641 gctcgcggct tggaccgggc cgctctatcc gctgcgcgac ggcaccgcga tcatcctcag
    17701 gatcctgcgc gagtcgggca agtcgcaggc gctggcggcg ccgcagggca tgtaccagca
    17761 gatgctcggc ggcaagaccg cgcagatgct gcgcctgcgg ctcgaccaca ccatgccgtg
    17821 cgtgcccgag atcagcgcca acaaatacgt cctcaacatc cgcttcctcg cacagaacgg
    17881 cgaagatccg aaagcgcgca ccgccgactg ggatgtcggc ttcgagctga cgttctgcaa
    17941 tctgtgaggg cagttccctg cccgcgctgc ggcacgctga gcgagtattc cgaggcgaac
    18001 aagtggcgcc cgttctgcag cgagcgctgc aggctgatcg acctcggcaa gtgggcctcc
    18061 gacgagtatc ggttgcccga cgacaagaaa tctagctcca agccccccga atcatagcga
    18121 ggccgtgggc gccggcgcgc caggcgtcct gcagatcggc gcgcgtgagg ccgccgatgg
    18181 cgtacaccgg aatcgtcgcc ccgcgcgcga gctcggcgaa gcgctgccag ccaagcgccg
    18241 gcgacttgtc tttcactggc ccgaggacga cgaaatcgag ctgcagctcc atggcccgat
    18301 ccagctccgc acgcgtgtgg caggaagccg cgaccaggcc ttccggtttc ccggtcaggc
    18361 gcatgagctg ggaagcggtg aagtgaatgc cgtcggcgcc cgggaaggcc gacttcacca
    18421 gcactttgca gccatggcga tgcgcaaggc cgatcgcctg ctcggtaaaa cgccttctct
    18481 cggattccac gagtccgggc tcgcgcacct gcagcaactt caatccctgc cggaggcgct
    18541 gctcgagccg cgcccgcatg tcggcgatcc cgtagtgcgc ggcattggta atcccgtatt
    18601 cggtcggcag ggcgagcgag gcgagtaccg gcgcgttggc cggcagcatc ggcgcaacca
    18661 tcggcgcgcc ggcgctctgc caggcaatgg cctggtcttc acgcggatgc ggctcgccct
    18721 tccattcatg cacccggaaa aagttgaggc gaaccgtccc gtgcgggtag gcgaacaccc
    18781 gtgtgatcca gggaaagggc ggtccgatgt cgatgcccag ctcctcgtgc agctcacggg
    18841 cgagcgcccg gtcggccggc tcgcccggct cgaccttgcc gccggggaat tcccagtagc
    18901 cggcgtacac cttgccttgc ggacgctgcg cgagcaggaa ggcgccatcg ccgcgttgga
    18961 tcactgcggc cgcaacttca atcacttttt cttggcgtgc ctgccggccc aatcgcgggc
    19021 aaattgatag gccacccgtc cgctgcgcga gccgcgctgc agcgcccact gaagcgcctc
    19081 ttccttggat ttctcattgg aagaaacccc gaaggatttg agccattgct caacgatctc
    19141 caggtactcg tcctggtcga acggatagaa ggtcacccac agcccgaagc gctcggagag
    19201 cgagatcttc tcctcgacgg tctcgcccgg atggatctcg tcgccgacgt acttggtctc
    19261 gaggttctcg gccatgtact ccggcatcag gtggcggcgg ttcgaggtcg cgtagatcag
    19321 caggttctcc gatgtggtgg agatcgaccc gtcgagcgcc accttgagcg cgatataccc
    19381 ctcctccgcg ccgtggaagc tcaggtcgtc gcagaagagg aggaagcgct cggggcgcgg
    19441 cgcgacctgg tcgacgatgg tgggcaggtc gatcaggtcg ttcttctcga cctcgatcag
    19501 ccgcaggccc tgcttcgaat acttgttgag caaccccttg acgatggagg acttgccggt
    19561 gccgcgcgcg ccggtgagga gcacgttgtt ggccggcctg ccgtcgatga actggcgcgt
    19621 gttctgctcg acgcgctcga tctgcttgtc gatgccgcgc aggtcggaaa gcttgatgcg
    19681 gtggacgtgg cgcaccggct gcagccatcc cgggcggcct cggccgcccg agcgccagcg
    19741 g
//
LOCUS       DATBSZ010000088        24345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_149086, whole genome shotgun sequence.
ACCESSION   DATBSZ010000088 DATBSZ010000000
VERSION     DATBSZ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 24345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24345
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149086"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VIG70_05785"
     CDS             <1..338
                     /locus_tag="VIG70_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY8250098.1"
                     /translation="AVIYILLGTALDGVSMIVLTTSIVIPMVEKAGFDLVWFGIFIVL
                     LVEIAELTPPLGFNIFVLQTMTGRDSNYVALASLPFFCMMVLCIALITAFPALATWLP
                     DALIGATRK"
     gene            335..1018
                     /locus_tag="VIG70_05790"
     CDS             335..1018
                     /locus_tag="VIG70_05790"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEY8250099.1"
                     /translation="MKRAHLEAARDTVVVTVRQRCFWLFAALLLLIVSVPFLEETPYG
                     RIALNVTTLLVLISGAAAIGRSAGAIVITVLLAMPTAVFLFLAVVQGQKHFMIYSQAF
                     GAAFFFAITSYLLSYTLRRDVMTMDKLYGAAAAFLMLGVLWAYLYAILLAFYPGALLM
                     NGAPMTAWLPSTMLYFSFVTLTATGMSDIMPAHAVARMLCALEMITGILFIAVLIARL
                     AGSYPPPER"
     gene            1033..1653
                     /locus_tag="VIG70_05795"
     CDS             1033..1653
                     /locus_tag="VIG70_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY8250100.1"
                     /translation="MILDVGGVLAHDIWEPMFDDLAKQYRRNRKKLHAAGRLLWETFA
                     YVPETPANTWRDMQRRYWELFLLISRLPVTVASLIRLTDRYVIPVEGMRPILEKLRAR
                     GTPLVICSNNNEIWWPRQAKKLQLKRFFGEERLVLSSRVGAPKASPRLEMFRAAVAAA
                     GVPAASCFFVDDRRENITRARRFGIDAMLFRGPEHFAAALRKRKLL"
     gene            complement(1670..2581)
                     /locus_tag="VIG70_05800"
     CDS             complement(1670..2581)
                     /locus_tag="VIG70_05800"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY8250101.1"
                     /translation="MLTLNALRAAPVALANWLGLSGRDERPTGRILLFHGTPQRDAAA
                     LERQLRWLKRRFSIVPLRSLVAAAKAGDALGRKVALTFDGGLRSNVAVAYPVLHRLAL
                     PATFFVCPGLVDRGAWLWMHEACRRLARLEPAARRELAAEWQAPAGVEAFVEWMKTLA
                     LDARRRVEARLCEATPSFVPTVAERGECDLASWQELRRLDPALVTIGSHTLSHAILPG
                     VPADELEVEVRDSRRRLERGLERPVEFFSYPNGDHNADTDACVRRHYRAAVTSSFGWV
                     RGGAAAHRLPRRVAPTGVLRLAREIYA"
     gene            2626..4035
                     /locus_tag="VIG70_05805"
     CDS             2626..4035
                     /locus_tag="VIG70_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY8250102.1"
                     /translation="MQLHGPIYLTEDIRLMERAAGDGAALMERAGAAAADIAVPLAGD
                     KGKDVLVLAGPGNNGGDARIVAERLRERYFRVHLASQLDQIPQKSWGLVVDGLFGIGL
                     ARDIEGGQAALVDYANRQRCPVLALDIPSGLHADTGRVLGRAVRATHTVTFIALKPGL
                     LTLDGPDHCGEIVVSELDLAVTELAAPTAWVAEPGLFRNLLKPRPRNFHKGMAGSLAI
                     LGGAAGMSGAALLAGRAALKMGAGRVYVGLLEENLSLDFQNPELMLRHPDDALGQDLD
                     ALVVGPGIGQGERAETLVGAALASDLPCVVDADALNLISENEDLRHACARRTADTLLT
                     PHPAEAARLLALSTADVQADRVKAAKILAANLNAHVVLKGAGSILVARDGHWFVNTSG
                     NPGMASAGMGDVLAGILGALLAQRYSGESSLVLGVHLHGAAADDLVKKGIGPIGLTAG
                     EVIDAARRTWNGWLAGEAR"
     gene            complement(4015..4839)
                     /locus_tag="VIG70_05810"
     CDS             complement(4015..4839)
                     /locus_tag="VIG70_05810"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HEY8250103.1"
                     /translation="MLHITNGDHAVAVLSGVVSGTLLPWRDVLHEGPVRAGLSLEELS
                     RERAAFIAAAGWGALPAVEREFQARDAAFRRAGEHDEIVLWFEHDLYDQLQLIQVLEG
                     LSALRGPPISLVCEAEYLGRMKPERAAELFALRNPVTRRHFQEAGAAWAAFRAPDPRA
                     LQNLKTTSLPFLAAALRRHLQEFPWTTDGLSLLERRIVQALEGGPLPFAQLFRAVEED
                     PAFLGDAVLEWHLQRLREEGLLEGKKRRRRLPRWLGGHEVKDETLRWDPALARLTG"
     gene            complement(4850..5191)
                     /locus_tag="VIG70_05815"
     CDS             complement(4850..5191)
                     /locus_tag="VIG70_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250104.1"
                     /translation="MKRALLFLGIVFHVPVFALSPAAQEFMAISKELEPVQCEKRRLR
                     REIAFAEAEQRDADAKAARARFARLDKDPKTARLEKRLAELEPRIKKSSDPEDLRAIS
                     LQQREAFYRCE"
     gene            complement(5188..5586)
                     /locus_tag="VIG70_05820"
     CDS             complement(5188..5586)
                     /locus_tag="VIG70_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8250105.1"
                     /translation="MHDAQGVQLQPASDVTYRHGGYGELLEALERALEGKAPGESIRL
                     QLEPEQAFGEYDETLLRVEPAERYGDGVAVGMEVEEDSRFYTVTDIAEGKVVLDGNHP
                     LAGMALRFSVEVIAVRSASPEEIRQGVAPA"
     gene            complement(5619..6092)
                     /locus_tag="VIG70_05825"
     CDS             complement(5619..6092)
                     /locus_tag="VIG70_05825"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY8250106.1"
                     /translation="MLEPGQPAPSFELPDADMQEVSLSAFKGKKHVVLYFYPKDGTPG
                     CTMVTTEFSDHEDEFGKCDCVIMGLSRDDCLTHAEFRDRNGVTISLLSDPEGEVCRKY
                     GVIQEKEVTEGVRRECLVRSTFVIDKKGVIRHAAYGVNPRGHAAEVFDLVRRLRS"
     gene            6220..7209
                     /locus_tag="VIG70_05830"
     CDS             6220..7209
                     /locus_tag="VIG70_05830"
                     /inference="COORDINATES: protein motif:HMM:NF042820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8250107.1"
                     /translation="MRSYWQKRPLFVRGAIPGFSGIIDRNGLAALAARDDVESRIVER
                     RGRRLETVPGPFPRVKAKSSGWTLLVNGVNLHVPGADALLQRFRFVPQARLDDVMVSY
                     ATPGGGVGPHVDHYDVFLLQGPGRRAWRVENRRFVVEKGDLLYLPPGVRHDGVALEPC
                     FTYSIGFRAPRGAELGAAFLDWLHERGLPQAEYRDPGIAPARRSAEVPRSMAAFAQAL
                     LSRIRWSRRDVDRFLGEYLSEPKPHVVFKPANSRRSLAHSLVRLDPKTRLLYRGRRFF
                     INGESVELGRSSAARLRELADRRMVHGARLAHAPLAGLISTWRGLGYLSLEKI"
     gene            7210..7695
                     /locus_tag="VIG70_05835"
     CDS             7210..7695
                     /locus_tag="VIG70_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250108.1"
                     /translation="MDAPIYERFDTNEGFQASIERLLEQPGRELRVFDPDGAALRLND
                     PPRIEALGRFLLASRTRRLYLVVHSTDHVTRQCPRLMMLLRRYSHAMQINRTHEEIRE
                     VQDAFLLLDSMHYVRRPVATLFRGAMGLGDENEGQALRGRFGEIWSASYPAVSSTTIG
                     L"
     gene            complement(7764..8534)
                     /locus_tag="VIG70_05840"
     CDS             complement(7764..8534)
                     /locus_tag="VIG70_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8250109.1"
                     /translation="MRFTSLASGSSGNCLVAEAAGTVVLVDCGLSLTETERRLARAGL
                     QPSQVSALLVTHEHGDHACGAFEFAAAHRIAVYLTHGTLAALKAEGKVLDGVALSIVN
                     GRQSFFVDGIQVLPFTVPHDAREPVQYVLSDGASRLGVLTDVGISTSHVEKMLSGLDA
                     LVLECNYDRDLLWNGGYPRWLKERIAGPFGHLDNRESERLLAALDRSRLKHVIGAHLS
                     QQNNRPELARAALARALGCEESWIGIAGQDDGFAWRSI"
     gene            complement(8550..9713)
                     /gene="bamC"
                     /locus_tag="VIG70_05845"
     CDS             complement(8550..9713)
                     /gene="bamC"
                     /locus_tag="VIG70_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEY8250110.1"
                     /translation="MYRFLAITAATAVLSGCSAFKDTSAVDYKSAGKLPPLEVPPDLT
                     SPQRDNRYSIPESARSSATLSGYEAERRDPSRAAAARSSASGAPVVLPEVERMKIERA
                     GTQRWLVVQEPPEKLWPLVKDFWQENGFLIKTEMPEAGVMETDWAEYRARIVESPVRD
                     FLGKLAESLRSTSERDKYRTRLDRTADGKGTEIYISHRGMQELYTTREATAGTPGQTA
                     WQPRPPDPELEAEFLRRLMVRLGAPEERAKQLAAGGQPPQQRAEIVKSNDGTERLQVY
                     EPFDRAWRRVGLALDRVGFTVEDRDRQKGLYFVRYADPESEMKEKDKGLFGRLFSWGS
                     DSKVKPEQYRVQVSQETAGEASGSQVFVLNKDGSAERSKTGQRILTLLHEQLK"
     gene            complement(9713..10621)
                     /gene="dapA"
                     /locus_tag="VIG70_05850"
     CDS             complement(9713..10621)
                     /gene="dapA"
                     /locus_tag="VIG70_05850"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY8250111.1"
                     /translation="MITGSLVAIITPMHEDGRLDLARFRQLIDWHAAEGTDGIVVVGT
                     TGESPTVDFDEHKELIRIAVQHSRGRIPVIAGTGGNSTAEAIELTASAKQNGATACLS
                     VVPYYNKPTQEGLYRHFRKIAETVDLPMIVYNVPGRTVADLANDTTLRLAEVPGIIGI
                     KDATANIERGTDLLKRAPRNFAIYSGDDATALALVLLGGHGVISVTANVAPKLMHQMC
                     AAALVGDVKKARELNLKLFALHQRLFVEANPIPVKWALAEMGMIEPGLRLPMSPLAEK
                     FHQTVREALHEAGIALPGLRVVEGRG"
     gene            complement(10618..11139)
                     /locus_tag="VIG70_05855"
     CDS             complement(10618..11139)
                     /locus_tag="VIG70_05855"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8250112.1"
                     /translation="MTHDCLETPSPWVVRWAPLVTRGPVLDVACGGGRHARLFLERKL
                     PVVAVDREAQVLPEGIRFVKADLEDGSPWPFAGQRFGAIVVTNYLHRALFPDLVAALG
                     EDGVLIYETFMIGNERFGRPSNPNFLLRPGELLEAFGVLTLVAFEQGTTQRAVVQRIC
                     VIRGDASRARIPA"
     gene            complement(11120..11488)
                     /locus_tag="VIG70_05860"
     CDS             complement(11120..11488)
                     /locus_tag="VIG70_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEY8250113.1"
                     /translation="MIRVVRLGSPRASGEGLRLGTVRRPPRGVKKSEFARRDYYDVWL
                     PDLSPSAPLIAKAHRDWTAFVKAYRREMSKPAAQRLLELLARLSHHAEFSVGCYCEDE
                     SRCHRSILRQLLAENDARLS"
     gene            complement(11463..11780)
                     /locus_tag="VIG70_05865"
     CDS             complement(11463..11780)
                     /locus_tag="VIG70_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250114.1"
                     /translation="MNAFAQEPSFSGGDGSSCEAAVVPLAAEGMVQSQYHWLRKTFGG
                     GTLVRQTLGASTDGKRRYDLILWRKPDGQTAEICFDVTTVFEETIRRVEEQESGDSRR
                     PAR"
     gene            complement(11819..12235)
                     /locus_tag="VIG70_05870"
     CDS             complement(11819..12235)
                     /locus_tag="VIG70_05870"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8250115.1"
                     /translation="MGPIREGGCLCGAVRYRAQGNPARTVVCHCRFCQRYTGSAFGVW
                     PTYVPGKVELRGTLSTYEHRSDESGRWIRLNFCPRCGTTLTSTFEKGPGEVAILGGTF
                     DDTGWITVDRHVWTRSRQHWVPASAGVASFEKSSSG"
     gene            complement(12235..12522)
                     /locus_tag="VIG70_05875"
     CDS             complement(12235..12522)
                     /locus_tag="VIG70_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250116.1"
                     /translation="MGGLLPLPVIALYGLEGGIVFALSVVHLIVYLGVFYYLARRIRK
                     PVTAGIVLLALFALSFVPIYGSGENLASGGKLSLNAFQAYQQAFVEVFAKR"
     gene            complement(12641..14404)
                     /locus_tag="VIG70_05880"
     CDS             complement(12641..14404)
                     /locus_tag="VIG70_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8250117.1"
                     /translation="MVLATTSKTSALAGLLVAAAFLYLARDVLIPLALAILLAFLLAP
                     MVRQLERLRLGRIASTFIAVVIAFSVIGGVGWVAARQALSLAAKLPEYRENIREKIRA
                     VRAPQNGTIGRATEAIKDLENEAAPGAEPMAVTETAPTAFAALRELVAPFVQPIGTAL
                     AVIVFTILLLLNRENMRERLIGLVGARKINVTTQALEEASYRVSSYLYMQLVVNACFG
                     IPFAIALWLIGIPNAMLWGLLGTLLRFVPYAGVWVAVALPATLAFAIFDGWTHVAWVL
                     GTFLALELILVNVVEPWLYGRSAGLSPIAIIAAAIVWTWLWGPVGLLLAIPLTVCVAV
                     LGRYIPEMGYLNVLLGVEPVLSPEARFYQRLIARDQDEAITLAEEYAGEHGAAGLFDT
                     LLIPALALIETDRHKGALEPESERFAFDTTRQILEEMQTEETSAGAAPCSGVCIVPAR
                     DEADELAGAMLARLLPGAQLLSSDSLAAEMLEQVGKGACRLVCISAVPPHAASHAAYL
                     ARRLKKQLPELKVVVALWTSEGIDKIKPRLLSAGVDEVFTRLADVQSHLRQLAGPAKP
                     ATAKQGQTTISETRKGTARSN"
     gene            complement(14382..16526)
                     /gene="glgX"
                     /locus_tag="VIG70_05885"
     CDS             complement(14382..16526)
                     /gene="glgX"
                     /locus_tag="VIG70_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY8250118.1"
                     /translation="MNAPELRLARVKEGLPYPRGATWDGKGVNFALFSAHATKVEVCL
                     FDREGKETERIELPEYRDETFHCYMPDIGPGTFYGYRVHGPYAPDEGHRFNPNKLVLD
                     PYARAHIGELRGSPNWDPAVFAYKLETGDDTTFDERDSAPFMPKCVVVDPDFNWKRER
                     VWRPVPWENTIVYETHVRGYTKLHPKVPEHQRGTYAGLAVKEVIDYIKSLGVTSVELL
                     PVHTFINDEFLLKRNLTNHWGYNSIGFFAPDPRYAADPSKSLQEFKEMVARFHDAGLE
                     VIIDVVYNHTAEGNELGPTLSFKGIDNASYYRLMPDKKRYYINDTGTGNTLNLSHPVV
                     IQMVTDSLRYWAEEMHVDGFRFDLGTILAREPGGFDTHNGFMKAVMQDPVLHRVKLIA
                     EPWDCGPGGYQVGGFPPGWAEWNDKFRDTVRDYWRLEATPAELAPRLCGSPDAFMRRG
                     RQPWASVNFITAHDGFTLNDLVSYNDKHNEANGEGNKDGHSHNRSWNHGAEGPTDDEA
                     IRGLRKRQMKNLLATLLLSQGTPMLLAGDEFAHTQNGNNNAYCQDNEISWLRWDHDDR
                     SQRLIRFVQKLTGLRSRYPILRRTRFLSGQFDEDLGVKDVTWINTTGEAMREENWKDP
                     NARVFGMLLDGRAQTTGIRKRGEEATLLIIFNPWQDVVRFKLPPSNGGGDWTLLADTN
                     FPDSKEEPRFPIGHEYQVTARSLLLFGLSHNK"
     gene            16635..16931
                     /locus_tag="VIG70_05890"
     CDS             16635..16931
                     /locus_tag="VIG70_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEY8250119.1"
                     /translation="MFDTRSWDRFVHTTPGRRFQERYRRMQHEKGSAWKRCAFVLAGI
                     ALSVVGLFFMAVPGPGIPILAVGLALIAQESSVMARLLDRAEIRLRRWWKRIRS"
     gene            complement(16928..17053)
                     /locus_tag="VIG70_05895"
     CDS             complement(16928..17053)
                     /locus_tag="VIG70_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250120.1"
                     /translation="MHRLWPKALLIAVFAFATAAAFAAYLKPGMLVEFANLVLCS"
     gene            complement(17034..18230)
                     /locus_tag="VIG70_05900"
     CDS             complement(17034..18230)
                     /locus_tag="VIG70_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8250121.1"
                     /translation="MNPGTVLVCGCMILFLAFGARAGFGLYLQPISLEFGWGREVLSF
                     SLALQQIVWGALGPLAGIVADRYGTGRVVAACGALYTLGLIGMAFAQTPTAMHFAAGL
                     LVGAALAGATFATVLAVIGRTVAPERRSTALGVATAAGSFGQFALLPVNQLLIGAFNW
                     HVALLIMAGMTALIMPLAAALSSRPAAAPGTGQSLTAALREAMRERGFHLLFWGYFVC
                     GFHIAMLTVHLPSFVTDAGLSATHGMTALALIGLFNIIGTLSAGWLGGRFSKKYILSS
                     IYSVRGVLIALLVFFPLSPLTLYVFACGIGLLWLGTVPLTNGLVGQIFGLRYMATLTG
                     LVFFGHQIGSFVGVWLAGYLYDTTGSYLGAFYTSIGLSIFAALINLPVNEKPLAERGK
                     HAQALA"
     gene            complement(18291..19580)
                     /locus_tag="VIG70_05905"
     CDS             complement(18291..19580)
                     /locus_tag="VIG70_05905"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY8250122.1"
                     /translation="MIWVMLGLLLATMLIGIPILLCIALIGFIGIAAEPGVVMPMFAQ
                     KSFAQLDSYTLLALPFFILAGALMTAGGMSEQLVDFSRVLVGHLKAGLAHASVVCSMI
                     LSGVSGSSTADASAIAQVLVPTMKQNGYKAGFAAALIATAGTIGAIIPPSMTMVVYGA
                     IAQVSIGGLFLGGILPGLLIGFFLMGVVKIYTYHPAYPELRAVHGSFEFQKVLSSLKR
                     VWPALLGPLVIVGGILGGIFTATEAGVVACLYAYLVGAFWYRKIRLGDLRDIFVEAAI
                     TTTMVSAVIAISGAMGWLLAYMQFNDMAVAWVTTASKNAYVVLLVFALVMILLGTFVD
                     SLAILLVFAPVAVELCKRYGIDPIQMGLVMVICNQIGAVSPPTAPLLFVTTNIAKTSL
                     DDTTRHVWPFFAAECLALVVVIFVPGLSTWIPRYFLG"
     gene            complement(19577..20044)
                     /locus_tag="VIG70_05910"
     CDS             complement(19577..20044)
                     /locus_tag="VIG70_05910"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY8250123.1"
                     /translation="MKRAFAWIDRGAEYGVAAIFAAMCAVGLLQVFNRFVLNRSLSWS
                     EEFQIYCHVWIVFLGIPIAYRRGAHLAVESFRNKFPPKVGKAFDYFIEILWIWFAVAL
                     AWLAWRVSEVAKLQSSPGLELPMNYVYYSMVVGGVYLLIVVLRRVFLKETPDP"
     gene            complement(20041..21018)
                     /locus_tag="VIG70_05915"
     CDS             complement(20041..21018)
                     /locus_tag="VIG70_05915"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8250124.1"
                     /translation="MKRTFMLLLLMTFALTATAQTKKLLRIHTAGPNDTNVDNTKLAV
                     EFQDRANAAQSTVEIKVFPASQLGQTREVIEAMRLGSGAAGTTGGPAEYASFVRRLGV
                     LGLPFEWKSYDHALQVLDGPVGKELDQDMEKAGFKVLAWAVSWGYRNVITSKKEVKTA
                     ADLRGLKIRTIPTKVFVAAINSMGANATPMNFGEIYTSLQSGVLDGYEHTAATTISFK
                     MYEVACCMALTKHLMDPTFLVFSLAEWKKLSAPEQAALQKVAIEAGKIVREMAPQREG
                     QALAEVKRLGMKVNEIDTAPLVKAALAAQDELAKEFGAEKLLEQIRKTP"
     gene            complement(21042..21962)
                     /locus_tag="VIG70_05920"
     CDS             complement(21042..21962)
                     /locus_tag="VIG70_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8250125.1"
                     /translation="MTLTELRYIVAVARERHFGRAAETCFVSQPTLSVAVKKLEEELG
                     VILFERGPGEVSVTPSGQKIIEQAQRVLEEASRIREIAAAGRDPLAGPLRLGAIYTIG
                     PYLLPKLIPILRRTAPAMQLLIQENFTHRLADELKNGEVDVILVALPFEEPGVLTRAV
                     YDEPFLVAVPKGHPWEGRKRVTSEELTSESLLLLGEGHCFRDQVLEICHTVRARERSP
                     LARTLEGGSLETIRQMVAGGVGVTVLPATSTGAGGAGDLIRILPFARPVPTRRVAIAW
                     RRSFPRPEAIEVLRKSILACNLPQVEKLAS"
     gene            complement(21959..23287)
                     /locus_tag="VIG70_05925"
     CDS             complement(21959..23287)
                     /locus_tag="VIG70_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY8250126.1"
                     /translation="MDGKQSTVAPKQLLTVWDGIILICGMVIGAGIFKAPSIVAGNTS
                     SGAEFLGAWVLGGAISLCGALVYAELATRHPETGGEYAFLSRGMGRGVAFVFAWARMT
                     VIQTGAIAAVAFVFGDYASEIFSFGKNSSAIYAALGVVALTALNVAGTVEAKGLQKVM
                     QFLLFAGLAFLVIAGLMAGAPAKPAVAASSGGSFGFAMIFVLLTFGGWNEAAYLSGEV
                     RDARRNMIRILIGGILTLTVIYLLVNVAYLAALGLGGMKESKAVAADVVRLVMGDKGA
                     IVIALIVCLAALTTMNAAIFTGARTTYALGRDFPFFGKLGAWRESGSTPANALILQGA
                     VTLVLVLASTFTPDGFSAMVAYTSPVFWTFFLLTALTLFVFRRREPQAPAFRVPLYPV
                     VPIAFCIACVYMLYSSVNYVRFAVEFGVAVFAGLAIMALGIPLYFLARNR"
     gene            complement(23263..24132)
                     /locus_tag="VIG70_05930"
     CDS             complement(23263..24132)
                     /locus_tag="VIG70_05930"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8250127.1"
                     /translation="MLDRHVARFLLPLLFLAAPAVAQPQTWAWDDGTVPYVQTPAEIV
                     ERMMRMAEVGRGDYLIDLGSGDGRIVIEAAKRGARGLGVDLDPSLVKLATENANGAGV
                     GERARFEVRDIFETDLSGATVVAFYLLPEFNAKLLPKLLKLKPGTRIVSHDGGIGDWA
                     PDERLEMRAPEKPVGVGGVSRVELWIVPADAKGSWASDLPAHGGRWNFRIKQTYQVLD
                     IEVGAQGRDLLVRNSRLRGEEIKVIVTGVVNGRAWHHYFVGTLRDDRITGEVTVSDGN
                     NKRSFPWTASRAR"
     gene            24193..24268
                     /locus_tag="VIG70_05935"
     tRNA            24193..24268
                     /locus_tag="VIG70_05935"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24226..24228,aa:Arg,seq:cct)
BASE COUNT         3997 a         8217 c         8161 g         3970 t
ORIGIN      
        1 tcgccgtgat ctacatcctg ctgggcaccg cgctcgacgg cgtctcgatg atcgtgctca
       61 ccacctcgat cgtcattcca atggtcgaga aggccggctt cgacctggtc tggttcggca
      121 tcttcatcgt gcttctggtg gaaatcgccg agctgacgcc gccgctgggc ttcaacatct
      181 tcgtgctgca gaccatgacc gggcgcgaca gcaactacgt ggcgctcgcc tcgctgccgt
      241 tcttctgcat gatggtgctg tgcatcgcgc tgatcaccgc gttcccggcg ctcgccacct
      301 ggctgcccga cgcgctgatc ggggcgaccc ggaaatgaag cgcgcgcatc tcgaagccgc
      361 gcgcgatacg gtcgtggtga ccgtgcgcca gcgctgcttc tggctgttcg ctgcgctgct
      421 gctgctgatc gtgtcggtgc cgttcctgga ggagacgccg tacggtcgca tcgctctgaa
      481 cgtcacgacc ttgctggtgc tcatctccgg cgccgccgcg atcgggcgaa gcgccggtgc
      541 catcgtgatc acggtgctgc tagccatgcc gaccgcggtg ttcctgttcc tcgccgtcgt
      601 ccaggggcag aagcatttca tgatctattc gcaggcattt ggcgccgcct tctttttcgc
      661 gatcaccagc tacctgctga gctacaccct gcggcgcgac gtcatgacga tggacaagct
      721 ctacggtgcc gccgccgcgt ttctaatgct gggcgtcctg tgggcctatc tctacgccat
      781 cctgctcgcg ttctaccccg gcgcgctgct gatgaacggc gcgccgatga cggcctggct
      841 acccagcacc atgctgtatt tcagcttcgt cacgctgacc gccaccggca tgagcgacat
      901 catgccggcg cacgcggtgg cgcggatgtt gtgcgcgctc gagatgatca ccgggatcct
      961 gttcatcgcc gtgctgatcg cgcgccttgc cggtagctat cccccgcccg agcgctgatg
     1021 gccgcccgag cggtgatctt ggacgtcggc ggcgtgctgg cgcacgacat ctgggagccg
     1081 atgttcgacg acctcgcgaa gcagtaccgc cggaaccgca agaagctcca cgcggcgggc
     1141 aggctgctct gggaaacctt cgcctacgtc ccggaaacgc ccgccaatac ctggcgcgac
     1201 atgcagcgcc gctattggga actgttcctt ctgatcagcc ggctgcctgt tacggtcgcc
     1261 tccctcatcc gcctcaccga ccgctacgtg attcccgtcg agggcatgcg cccgatcctg
     1321 gagaagctgc gcgcgcgcgg cacgccgctc gtcatctgct cgaacaacaa cgagatctgg
     1381 tggccgaggc aggcgaaaaa gctccagctg aagagattct tcggggagga gcggctcgtc
     1441 ctgtcgtcgc gcgtcggcgc gccgaaggcg agcccgcgcc tggagatgtt tcgggccgcg
     1501 gtcgcggcgg ccggcgtgcc ggccgcgagc tgcttcttcg tcgacgaccg ccgggaaaac
     1561 atcacgcggg cccggcgttt cggcatcgac gcaatgctgt tccgcgggcc ggagcacttc
     1621 gccgccgcgt tgcgcaagcg caagctgctc tagtccgcag gactgttcct caggcgtaga
     1681 tctctcgggc gagccttagg acgcctgtcg gcgcgacgcg cctagggagc cggtgcgcgg
     1741 ccgcgccgcc gcggacccag ccgaacgaac tcgtgaccgc ggcgcggtaa tgccggcgta
     1801 cgcaggcatc ggtatccgcg ttgtgatcgc cgttcggata ggaaaaaaat tcgaccggac
     1861 gctcgagccc acgctcgagc cgccggcggc tgtcgcgcac ctcgacttcc agctcgtcgg
     1921 ccggcacgcc cggcaggatg gcgtgcgaaa gggtgtgcga gccgatggtc accagcgccg
     1981 gatcgaggcg gcgcagctcc tgccagctcg ccaggtcgca ctccccgcgc tcggccacgg
     2041 tgggcacgaa gctcggcgtg gcctcgcaca ggcgcgcctc gacccgccgg cgcgcgtcca
     2101 gggccagcgt cttcatccat tccacgaaag cctcgacgcc ggcgggcgcc tgccactcgg
     2161 ccgcgagctc gcgccgcgcg gcgggctcga gccgcgcgag ccggcggcac gcctcgtgca
     2221 tccagagcca ggcgccgcgg tcgaccagcc cggggcagac gaagaacgtc gccggcaggg
     2281 ccaggcggtg caggaccggg taggcgaccg cgacgttgct gcgcaggccg ccgtcgaagg
     2341 tgagcgcgac cttcctgccg agcgcgtcgc ccgctttcgc ggcggcgacc agcgagcgca
     2401 gcggcacgat gctgaagcgg cgcttgagcc agcgcaactg gcgctcgagc gccgcggcgt
     2461 cgcgctgcgg cgtgccgtga aacagcagaa tccgcccggt cggacgttcg tcccggccgg
     2521 agagcccgag ccagttggcc agggcgaccg gcgcagcgcg cagcgcgttg agggttagca
     2581 tcgactcggg tgggggcaag ggacgttccg cttagaatcc tagcggtgca actgcacggt
     2641 ccgatctact tgaccgagga cattcggctc atggagcgcg ccgcgggcga cggggccgcg
     2701 ctcatggagc gggccggcgc cgccgccgcg gatatcgcag tgccgctggc aggcgacaaa
     2761 ggcaaggacg tgctggtgct cgccgggcct ggcaacaacg gcggcgacgc acgcatcgtg
     2821 gccgagcggc tcagagagcg ctacttccgg gtgcacttgg cttcgcagct cgaccagatc
     2881 ccgcagaaat cctggggcct ggtggttgac ggtctcttcg gcatcggcct cgcgcgcgac
     2941 atcgagggcg gccaggcggc gctcgtcgac tacgccaacc gccagcgctg cccggtcctc
     3001 gcgctcgaca tcccgagcgg cctgcatgcc gataccggcc gcgtcctcgg ccgcgcggtg
     3061 cgcgccacgc ataccgtcac cttcatcgcg ctcaagcccg gcctgctgac gctcgacggc
     3121 ccggatcatt gcggcgagat cgtcgtttcc gagctggatc tcgccgtgac ggagcttgcg
     3181 gccccgaccg cgtgggtcgc ggagccgggc ctgttccgca atctcctcaa gccccggccg
     3241 cgcaatttcc acaaaggaat ggccggctcg ctcgccatcc tcggcggcgc tgccgggatg
     3301 tccggtgccg ccctcctcgc cggccgcgct gcgctgaaaa tgggcgctgg cagagtgtac
     3361 gttggcctgc tcgaagagaa tctttccctg gacttccaaa acccggagct gatgctgcgc
     3421 catcccgacg acgcgctcgg acaggacctg gacgcgctgg tggtcggccc cggcatcggc
     3481 cagggtgagc gcgccgagac gctggtcggc gccgcgctcg cgagcgacct cccgtgcgtc
     3541 gtagacgccg acgctttgaa tctgatttca gaaaacgagg acctgcgcca cgcctgcgct
     3601 cggcgcactg cggatacgct gctcacgccg caccccgccg aggccgcccg gctgctcgcc
     3661 ctgagcaccg ccgacgtgca ggcggatcgg gtgaaggcgg ccaagatcct cgcggccaat
     3721 ctcaacgcgc acgtagtgct caaaggtgcg ggcagcattc tggtggcgcg cgacggccac
     3781 tggttcgtca acaccagcgg caaccccggc atggcgagcg cgggcatggg cgacgtgctc
     3841 gcgggaatcc tcggcgccct cctcgcccag cgctattcgg gcgaaagctc gctcgtcctc
     3901 ggcgtgcacc tgcacggcgc ggcggccgac gatctcgtca agaagggaat cgggccgatc
     3961 ggcctcactg cgggcgaggt gatcgacgcc gcccggcgaa cctggaacgg ctggctagcc
     4021 ggtgaggcgc gctagcgccg ggtcccagcg cagcgtttcg tctttcactt catggccgcc
     4081 gagccaccgc ggcaggcggc gccggcgttt cttgccctcc agcagccctt cctcgcgcaa
     4141 gcgctgaagg tgccattcca gcaccgcgtc acccagaaac gccggatcct cctccaccgc
     4201 gcgaaacagt tgtgcaaacg gcagcggccc gccttccagc gcctggacga tgcgccgctc
     4261 gaggagcgac aggccgtcgg tggtccacgg aaattcctgg agatggcggc gcagcgccgc
     4321 ggcgagaaac ggaagcgacg tggttttcag gttctgcagc gcgcggggat cgggagcgcg
     4381 aaacgccgcc caggctgcgc ccgcctcctg gaaatgccgc cgcgtgaccg gattgcgcag
     4441 cgcgaacaac tcagccgcgc gctcgggctt catgcggccc aggtattccg cctcgcacac
     4501 cagcgagatc ggcggcccgc gcagggcgga caggccttcg agcacctgga tgagctgcaa
     4561 ctggtcatag aggtcgtgct cgaaccacag cacgatctcg tcgtgctcgc ccgcgcggcg
     4621 gaaagccgca tctcttgcct ggaattccct ttcgactgcg ggaagcgcgc cccagcccgc
     4681 cgcggcgatg aacgccgcac gctcgcgcga caactcctcc agcgagaggc cggcccgaac
     4741 cggcccttcg tgcagcacgt cgcgccacgg caggagggtc cccgatacga ccccggaaag
     4801 caccgcgacg gcgtgatcgc cgttggtgat gtggagcaaa ccggtgtccc tattcgcagc
     4861 ggtagaacgc ctcgcgctgc tggaggctga tcgcccgcag gtcctctgga tcggagctct
     4921 tcttgatgcg cggctcgagc tcggccagcc gcttctcgag ccgcgcggtc ttcgggtcct
     4981 tgtccagccg ggcgaagcgg gcgcgcgctg ctttcgcatc ggcatcgcgc tgctcggcct
     5041 cggcgaaggc gatctcgcgc cgcaggcgcc gcttctcgca ctgcaccggt tcgagctctt
     5101 tggagatcgc catgaattcc tgggctgccg gactcagggc gaaaaccggg acatggaaaa
     5161 cgatccccaa gaaaagcagc gctcgcttca cgccggtgct actccctgcc tgatctcctc
     5221 agggctcgcg ctgcgcacgg cgatgacttc gaccgagaac ctgagagcca ttccggcgag
     5281 cggatggttg ccgtcgagaa ccaccttgcc ctcggcaatg tcggtcaccg tgtagaagcg
     5341 ggaatcctct tcgacttcca tgccgaccgc gacgccgtcg ccatagcgct cggcaggctc
     5401 gacgcgcagc agcgtctcgt cgtactcgcc gaacgcctgc tcgggctcga gctgcagccg
     5461 gatcgactcg cccggcgcct tgccctccag agcgcgctcg agcgcctcga gcagctcacc
     5521 gtagccgccg tgccggtagg tgacgtcgga cgcgggctgg agctgcacac cctgggcgtc
     5581 gtgcatctcg atgcgcaggg aaacgacggt gttcccggct acgagcgcag gcgcctcacc
     5641 aggtcgaaca cttcggcggc gtggccgcgc ggattgacgc cgtaggcggc gtggcggatg
     5701 acgcctttct tgtcgatgac gaaggtcgag cgcaccaggc actcgcggcg cacgccttcg
     5761 gtgacctctt tttcctggat gaccccgtat ttgcggcaga cctcgccctc gggatcggac
     5821 aagagcgaga tcgtcacgcc attgcggtcg cggaattcgg cgtgggtcag gcaatcgtcg
     5881 cgcgacaggc ccatgatcac gcagtcgcac ttgccgaact cgtcctcgtg gtcggagaac
     5941 tcggtggtga ccatggtgca gccgggggtc ccgtccttag ggtagaagta cagcaccacg
     6001 tgcttcttgc ccttgaaggc cgacaggctg acttcctgca tgtcggcatc gggcagttcg
     6061 aacgacggag caggttgtcc cggttcgagc atggtgcgac gattctattc caattctttt
     6121 ttactttgac aagtgcgccg gcgcgtttgt cgctcgcgcg ctacatataa ttcctcgatg
     6181 aactcgcgaa tgctcggcgg cttgacgccg cgagattttt tgcgaagtta ttggcagaag
     6241 cggccgcttt tcgtgcgcgg cgcgatcccc ggcttctccg gaatcatcga tcggaacggg
     6301 ctggcggcgc tcgccgcgcg cgatgacgtc gaatcccgga tcgtggaacg ccgcggccgg
     6361 cgcctggaaa ccgtgccggg tcctttccca agggtgaaag caaagtcttc cggctggacc
     6421 ttgctcgtga acggcgtcaa cctgcacgtt ccgggcgccg acgcgctgct gcagcgcttt
     6481 cgtttcgtgc cgcaggcacg cctggacgac gtgatggtga gctacgccac gccgggcggc
     6541 ggcgtcggcc cgcacgtcga tcactacgac gtattcctgc tgcagggccc cggccggcga
     6601 gcctggcggg ttgaaaacag gagatttgta gtagaaaagg gagatctcct ctatctgcca
     6661 ccgggagtgc ggcacgacgg cgtggcgctc gagccctgct ttacctattc gatcggcttt
     6721 cgcgcgccgc gcggcgccga gctcggcgcc gccttcctcg actggctcca cgaacgcggc
     6781 ctgccgcagg ccgagtatcg cgacccgggg attgcgcctg cgaggcgctc cgcagaagtg
     6841 ccgcggtcga tggcggcttt tgcgcaagcg cttctttccc gcatccgctg gtcgcgccgc
     6901 gatgtcgacc ggttcctcgg cgaatacctg agcgagccga agccgcacgt ggtgttcaag
     6961 ccggcgaaca gccgccgttc actcgcccac tcgctagtgc gccttgatcc caagacgcgg
     7021 ctcctgtacc gcggccggcg cttcttcatc aacggcgaaa gtgtggaatt gggccggagc
     7081 agcgcagccc gcctgcgcga gctcgccgac cgccgcatgg tgcacggcgc gcggcttgcc
     7141 catgcgcccc tcgccggtct aatctcgacc tggcgggggc tgggttacct gagcttggag
     7201 aagatctgaa tggacgcgcc gatctacgag cgcttcgaca cgaacgaagg cttccaggcg
     7261 agcatcgagc gcctgctcga gcagccgggt cgggagctgc gggtgtttga tccggacggc
     7321 gccgcgctgc ggttgaacga ccctccgcgc atcgaggcgc tcgggcgttt cctgctcgcg
     7381 agccgcacgc gccggctcta cctcgtggtg catagcaccg atcacgttac gcgccagtgc
     7441 ccgcggctga tgatgttgct gcggcgctat tcccacgcga tgcagatcaa ccgcacgcac
     7501 gaggagatcc gcgaagtgca ggatgccttc ctgctgctcg actcgatgca ctacgtgcgc
     7561 cggccggtcg cgacgctgtt caggggcgcg atgggcctgg gcgacgagaa cgaaggccag
     7621 gcgctgcgcg gccgcttcgg cgagatctgg tccgcgtcct acccggcggt gtccagcacg
     7681 accatcgggc tgtgattcgg ggtcagggtg cgaattcgtt tttacgtacg tgaaaacaga
     7741 attcgcaccc tgaccccgaa ttactagatg ctccgccagg cgaagccgtc gtcctggccg
     7801 gcgatcccga tccagctttc ctcgcatccc agcgcgcgcg cgagcgctgc gcgcgccaac
     7861 tcggggcggt tgttctgctg cgagagatgc gccccgatca cgtgcttgag gcgcgagcga
     7921 tcgagcgccg cgagcaggcg ctcggactcc cggttgtcga ggtgtccgaa cgggccggcg
     7981 atgcgctcct tgagccacct cgggtagccg ccgttccaca gcaggtcgcg gtcgtaattg
     8041 cattcgagca ccagcgcgtc gaggccggaa agcattttct cgacgtgcga agtggagatg
     8101 ccgacgtcgg tgagcacgcc gagcctggag gcgccgtccg acagcacgta ttgcaccggc
     8161 tcgcgcgcgt cgtgcggcac ggtaaacggc aggacctgga tgccgtcgac gaagaacgac
     8221 tggcggccgt tcacgatgct gagcgccacg ccgtcgagca ccttgccctc ggccttcagc
     8281 gcagccagcg tcccgtgcgt caggtaaacg gcgatccggt gcgcggcggc gaactcgaac
     8341 gcgccgcagg catggtcgcc gtgctcgtga gtgacgagga gcgccgagac ctgggagggc
     8401 tgcaggccgg cgcgcgcgag gcgccgctcg gtctcggtca gcgaaaggcc gcagtcgacc
     8461 agcaccaccg tgcccgccgc ctcggctact aggcagttgc cggagctgcc gctcgcgagc
     8521 gaggtgaagc gcaaacggtc tagttcaggc tatttcagct gctcatggag cagcgtcagg
     8581 atgcgctgcc cggtcttcga gcgctcggcg gagccgtcct tgttgagcac gaacacttgg
     8641 ctccccgagg cctcgcccgc ggtttcctgc gacacctgta cccggtattg ctcgggcttc
     8701 accttcgaat cgctgcccca ggaaaacagg cgcccgaaca gccccttgtc cttttccttc
     8761 atctcggact ccgggtcggc gtagcgcacg aagtacaggc ccttctggcg gtcgcggtcc
     8821 tcgacggtga agccgacgcg atcgagcgcg aggcccacgc gccgccaggc ccggtcgaac
     8881 ggctcgtaaa cctgcagccg ctcggtgccg tcgttgctct tcacgatctc ggcgcgctgc
     8941 tgcggcggct gcccgcccgc ggcgagctgc ttcgccctct cttcgggcgc gcccagccgc
     9001 accatcagcc ggcgcaggaa ctcggcctcg agctcgggat cgggcggacg cggctgccac
     9061 gcggtctggc ccggcgtgcc cgcagtcgcc tcgcgcgtgg tgtagagctc ctgcatgccg
     9121 cggtggctga tatagatctc ggtgcccttg ccgtcggcgg tgcggtcgag gcgcgtgcgg
     9181 tacttgtcgc gctccgaggt cgaccgcagg ctctcggcca gcttgccgag gaagtcgcgc
     9241 accggcgatt ccacgatgcg cgcgcggtac tcggcccagt cggtctccat caccccggct
     9301 tcgggcatct cggtcttgat gaggaagccg ttctcctgcc agaaatcctt gaccagcggc
     9361 cagagcttct cgggcggctc ctgcaccacc agccagcgct gcgtgccggc gcgctcgatc
     9421 ttcatgcgct cgacttccgg cagcacgacc ggcgcgccgc tcgcgctcga gcgtgcggcg
     9481 gcggcgcgcg agggatcccg gcgctccgct tcgtagcccg agagcgtcgc gctggagcgc
     9541 gcgctctcgg gaatcgagta gcggttgtcg cgctgcggcg aggtgaggtc gggcggcact
     9601 tcgagcggcg gcagcttgcc cgccgacttg tagtcgaccg ccgaggtgtc cttgaacgcg
     9661 ctgcaacccg acagcacggc cgtcgcggcc gtgatggcga ggaaccgata catcaacccc
     9721 ggccttcgac gactcttagt ccgggaagcg caatacccgc ttcgtggagc gcttcccgca
     9781 cagtctggtg gaacttctcg gcgagcggcg acatcggcag ccgcagcccg ggctcgatca
     9841 tgcccatctc ggcgagcgcc cacttgaccg gaatcggatt cgcctcgacg aacaggcgct
     9901 ggtgcagcgc gaacagcttc aggttgagct cacgcgcttt cttcacgtcg ccgacgagcg
     9961 ccgcggcgca catctggtgc atgagcttcg gcgccacgtt cgcggtcacc gagatcacgc
    10021 cgtggccgcc gagcagcacg agcgcgagcg cggtcgcgtc gtcgccgctg taaatcgcga
    10081 agttgcgcgg cgcgcgcttg aggaggtcgg tgccgcgctc gatattcgcg gtcgcgtcct
    10141 taatgccgat gatccccggc acctcggcga ggcgcagcgt ggtgtcgttc gcgaggtcgg
    10201 ccacggtgcg cccgggcacg ttgtacacga tcatcggcag gtccaccgtc tccgcgatct
    10261 tgcggaagtg gcggtacagg ccttcctgcg tcggcttgtt gtagtacgga accaccgaca
    10321 ggcaggcggt ggcgccgttt tgcttggccg acgcggtgag ctcgatcgct tccgcggtcg
    10381 agttgccgcc cgttcccgcg atcacgggaa tgcggccgcg cgagtgctgc acggcgatgc
    10441 ggatcagctc cttgtgctcg tcgaagtcca cggtcggcga ttcgccggtg gtgccgacca
    10501 ccacgatgcc gtcggtaccc tcggcggcgt gccagtcgat gagctgcctg aagcgcgcga
    10561 ggtcgaggcg gccgtcctcg tgcataggag tgatgatggc aaccaggctt ccggtaatca
    10621 tgcggggatt ctagcgcgac tcgcgtctcc ccggatgaca cagatgcgct ggaccaccgc
    10681 cctttgcgtc gtcccttgct cgaaggcgac gagcgtcagc acgccgaaag cctccagcag
    10741 ctcgcccggg cgcagcagga agttcggatt ggaaggccgg ccgaagcgct cgttgccgat
    10801 catgaacgtt tcgtagatca gcacgccgtc ctcgccgagc gcggcgacga ggtcgggaaa
    10861 aagcgcccgg tgcagatagt tggtgaccac gatggcgccg aagcgctggc cggcgaacgg
    10921 ccaggggctg ccatcttcca ggtccgcctt cacgaacctg atcccttccg gcagcacctg
    10981 cgcttcgcgg tcgaccgcca cgacggggag ttttctctcc agaaacaggc gcgcgtgacg
    11041 ccccccaccg caggcgacgt cgagcaccgg gccgcgcgtc accagcggcg cccatcgcac
    11101 cacccacggc gacggcgttt caagacagtc gtgcgtcatt ttccgccagc agctgccgca
    11161 ggatggaccg gtggcagcgg ctttcgtcct cgcaatagca gccaaccgag aactccgcgt
    11221 gatgcgacag gcgcgcgagc agctcgagca ggcgctgcgc agccggcttc gacatctcgc
    11281 ggcggtaggc tttgacgaac gcggtccagt cccggtgggc tttcgcgatc agcggcgcgc
    11341 tgggcgagag atcgggcagc cagacgtcgt agtagtcgcg ccgggcgaac tcgctcttct
    11401 tcacgccgcg cggcgggcgc cgcaccgtgc ccaggcgcag gccctcgccg ctcgcccgcg
    11461 ggctaccgag ccggacgacg cgaatcaccg gattcctgct cttccacgcg gcggatcgtc
    11521 tcctcgaaca cggtcgtcac gtcgaagcag atctcggcag tctgtccgtc cggcttgcgc
    11581 cacaggatga gatcgtagcg ccgcttgccg tcggtggatg cgcccagcgt ctgccgcacc
    11641 agcgtgccgc cgccgaaagt cttgcgcagc cagtggtatt gcgactgcac catgccctcg
    11701 gcggcgagcg gaaccacggc ggcttcgcac gacgagccgt cgccgccgga gaaggagggc
    11761 tcctgagcga acgcattcat gaccatcagg catgagagac atagcgcgac ccgcgccatc
    11821 aaccgctcga gcttttctcg aatgaggcca cgcccgcgga cgccggcacc caatgctgcc
    11881 tcgaccgcgt ccacacgtgc cggtcgaccg tgatccagcc ggtgtcgtcg aacgtgccgc
    11941 cgagaatcgc gacctcgccg ggtcctttct cgaaggtcga ggtgagcgtg gttccgcagc
    12001 gcgggcagaa gttcagccgg atccagcggc cgctctcgtc cgaacgatgc tcgtaagtgg
    12061 acagcgttcc ccgcagctcg accttgcccg gaacgtaggt cggccacacg ccaaatgcgc
    12121 tgccggtgta gcgctggcag aacctgcagt gacacaccac cgtgcgcgcg ggattccctt
    12181 gcgcgcggta gcgcaccgcg ccgcacaggc aacctccttc gcgaatcgga cccattacct
    12241 cttcgcaaac acctcgacga aagcctgctg ataggcctgg aaggcgttga gactcaactt
    12301 gcctccgctc gccagattct cgccgctgcc atagatcgga acgaagctta gcgcgaacag
    12361 ggcgagcagc acgatgcccg ccgtcacggg ttttcggatg cgccgggcca aatagtagaa
    12421 aacaccgagg tacacgatca ggtgaacgac tgagagggca aacacgatgc ctccctcgag
    12481 cccgtagagc gcgatcaccg gcagcggcag cagcccgccc acggccaaca tgtaccaggg
    12541 acaggggaac gcgaggacca gtccgatgaa ggtcgcgctg cgcacttctt taccgaaaca
    12601 gctccatttc cctgcacgtt acgccgcgcg cagcggtgaa tcaattcgac cgcgccgtgc
    12661 ctttccgtgt ttcggaaatc gtggtctgtc cctgttttgc ggttgccggc tttgctggtc
    12721 cggcaagctg gcgcaggtgc gactggacgt cggcgaggcg ggtgaagacc tcgtcgacgc
    12781 cggcgctgag gaggcgcggc ttgatcttgt cgatgccttc gctcgtccac agcgccacca
    12841 cgaccttgag ctcggggagc tgcttcttca ggcgccgcgc gaggtaggcg gcgtggctcg
    12901 cggcatgcgg cggcaccgcg gaaatgcaca ccagccggca ggcgcctttg cctacctgct
    12961 caagcatttc cgcggcgagg ctgtcggagg agaggagctg cgcgccggga agcaggcgcg
    13021 cgagcatggc gccggcgagc tcgtcggctt cgtcgcgcgc cggaacgatg cagacgccgg
    13081 agcacggcgc cgcccccgcc gatgtttcct cggtctgcat ctcttcgagg atctggcgcg
    13141 tggtgtcgaa cgcgaagcgc tcgctctcgg gctcgagcgc gcccttgtgc cggtcggtct
    13201 cgatgagcgc gagcgccggg atcaggagcg tatcgaaaag tcctgcggcg ccgtgctcgc
    13261 cggcgtattc ctcggcgagc gtgatcgcct cgtcctggtc gcgcgcgatg agccgctggt
    13321 agaagcgcgc ttccggcgac agcaccggct ccacgccgag cagcacgttg aggtagccca
    13381 tctccgggat gtagcggccc agcaccgcga cgcacaccgt gagcggaatg gcgagcagca
    13441 gccccaccgg gccccacagc caggtccaga cgatggccgc ggcgatgatc gcgatcggcg
    13501 agagcccggc gctgcggccg tacagccagg gctcgacgac attcaccagg atcagctcca
    13561 gcgccaggaa agtgcccagg acccaggcca cgtgggtcca gccgtcgaag atcgcgaacg
    13621 cgagcgtggc gggcagcgcc acggccaccc agacccccgc gtagggaacg aagcgcagca
    13681 gcgtgccgag caggccccac agcatcgcgt tcgggatgcc gatcagccag agcgcgatcg
    13741 cgaacggaat gccgaagcac gcgttcacca ccagctgcat gtacaggtag ctgctgacgc
    13801 ggtaggaggc ctcctccagc gcctgcgtgg tgacgttgat cttgcgcgcg ccgaccaggc
    13861 cgatcaggcg ctcgcgcatg ttttcgcgat tgagcagcag gaggatcgtg aacacgatca
    13921 ccgcgagcgc ggtgccgatc ggctgcacga acggcgcgac cagctcgcgc agcgcggcga
    13981 acgcggtcgg cgcagtttcc gtcaccgcca tcggctctgc gccgggcgcg gcttcgttct
    14041 ccagatcctt gatcgcttcg gtcgcccttc cgatcgtgcc gttctgcggc gcgcgcacgg
    14101 cgcggatctt ctcccggatg ttctcgcggt actcggggag cttcgccgcg agcgacagcg
    14161 cctgccgcgc cgcgacccag ccgacgccgc cgatcaccga gaaggcgatg accacggcga
    14221 tgaaggtgga ggcgatgcgc ccgagccgca gccgctcgag ctgtcgcacc atcggggcga
    14281 gcaggaacgc aagcaggatc gcgagcgcga gcgggatcag cacgtcgcgc gcgaggtaca
    14341 ggaacgccgc ggcgaccagc agcccggcga gcgccgaagt cttacttgtt gtggctaaga
    14401 ccaaacagca ggagcgagcg ggccgtgacc tggtactcgt ggccgatcgg gaagcgcggc
    14461 tcttccttgg agtccgggaa gttggtgtcg gcgaggagcg tccagtcgcc gccgccgttc
    14521 gacggcggca gcttgaacct cactacgtcc tgccacgggt tgaagatgat gaggagggtc
    14581 gcttcctcgc cgcgcttgcg gatgccggtg gtttgggcgc ggccgtcgag cagcatgccg
    14641 aacacgcgcg cgttgggatc cttccagttc tcctcgcgca tcgcttcgcc ggtggtgttg
    14701 atccacgtga cgtccttgac gccgaggtcc tcgtcgaact ggccgctcaa gaagcgcgtg
    14761 cggcgcagga tcgggtagcg gctgcgcagc ccggtcagct tctggacgaa acggatcagg
    14821 cgctgggagc ggtcgtcgtg gtcccagcgc agccaggaga tctcgttgtc ctggcagtac
    14881 gcgttgttgt tgccgttttg ggtgtgggcg aactcgtcgc cggcgagcag catcggcgtg
    14941 ccctgcgaca gcagaagcgt cgccagcagg ttcttcatct gccgcttgcg caggccgcga
    15001 atcgcctcgt cgtcggtcgg gccctcggcg ccgtggttcc acgagcggtt gtgcgaatgg
    15061 ccgtccttgt tgccctcgcc gttcgcctcg ttgtgcttgt cgttgtagct cacgaggtcg
    15121 ttgagggtga agccgtcgtg cgcggtgatg aagttgacgc tcgcccaggg ctggcggccg
    15181 cggcgcatga aggcgtcggg cgagccgcag agccgcgggg cgagctccgc cggcgtggct
    15241 tcgagcctcc aatagtcgcg caccgtgtcg cggaacttgt cgttccactc ggcccagccg
    15301 ggcgggaagc cgccgacctg atagccgccc gggccgcaat cccagggctc ggcgatcagc
    15361 ttcacccggt gcagcaccgg gtcctgcatc accgccttca tgaagccgtt atgggtgtcg
    15421 aagccgccgg gctcgcgcgc gaggatggtg ccgaggtcga agcggaagcc gtcgacgtgc
    15481 atctcctcgg cccagtagcg caggctgtcg gtgaccatct ggatcaccac cgggtgcgag
    15541 agattgagcg tgttgccggt cccggtgtcg ttgatgtaat agcgcttctt gtcgggcatc
    15601 agccggtagt acgacgcgtt gtcgattccc ttgaacgaca gcgtcggccc gagctcgttg
    15661 ccctcggcgg tgtggttgta gacgacgtcg atgatgactt cgagcccggc gtcgtggaag
    15721 cgcgccacca tctccttgaa ctcctgcagc gacttcgagg gatcggcggc gtagcgcggg
    15781 tcgggcgcga agaagccgat cgagttgtag ccccagtggt tcgtcagatt gcgcttcagc
    15841 aggaattcgt cgttgatgaa cgtgtgaacg gggagcagct cgaccgaggt gacgccgagc
    15901 gacttgatgt agtcgatgac ctccttcacc gcgaggccgg cgtacgtgcc gcgctggtgc
    15961 tcgggcacct tcggatgcag cttggtgtag ccgcgcacgt gcgtctcgta gacgatggtg
    16021 ttctcccacg gcaccgggcg ccagactctc tcgcgcttcc agttgaagtc cgggtcgacc
    16081 accacgcatt tcggcatgaa gggggcgctg tcgcgctcgt cgaatgtggt gtcgtcgccg
    16141 gtctcgagct tgtaggcgaa caccgcggga tcccagttgg gcgagccgcg cagttctcct
    16201 atgtgcgcgc gcgcataggg gtcgagcacc agcttgttcg ggttgaagcg gtggccttcg
    16261 tcgggcgcgt agggaccgtg cacgcggtag ccgtagaacg tccccggccc gatatccggc
    16321 atgtagcagt ggaaagtctc gtcgcggtac tccggcagct cgatgcgctc ggtttccttc
    16381 ccctcgcgat cgaacaggca gacctcgacc ttggtcgcgt gggcggagaa cagggcgaag
    16441 ttgaccccct tcccgtccca ggtggcgccg cgcgggtacg gcaggccttc cttgacgcgg
    16501 gcaaggcgca attcgggagc gttcatttgg ggcaagacaa tagattgcac ggaccgtgcc
    16561 cgcgccactt gggccatggt ccgggtggct catgggcggt agatgcgcgg cccggcgctt
    16621 gcttgaatcc ggccatgttc gacactcgga gctgggaccg cttcgtccac accacgccgg
    16681 gacggcgctt ccaggagcgc taccggcgca tgcagcacga gaaaggcagc gcgtggaaac
    16741 gctgcgcttt cgtcctggcc ggtatcgcgt tgtccgttgt agggctgttt ttcatggcgg
    16801 tgcccggtcc cggcatcccg atcctcgcgg tcggcctcgc gctcatcgcg caggagtctt
    16861 cggtcatggc gcgcctgctc gaccgcgccg agatccggct gcgccgctgg tggaagcgga
    16921 tccgctccta gctacagagc acgaggttcg cgaactcgac cagcatcccc ggcttgaggt
    16981 aagcggcgaa cgccgccgcg gtcgcgaacg cgaacacggc gatcagcaaa gccttaggcc
    17041 agagcctgtg catgctttcc acgctcggcg aggggcttct cgttcaccgg caggttgatg
    17101 agggccgcga agatgctcag cccgatcgac gtgtaaaaag cgccgaggta gctgccggtg
    17161 gtgtcataga gataccccgc gagccacacg ccgacgaagc tgccgatctg gtggccgaag
    17221 aacactagcc ccgtgagggt cgccatgtag cgcaggccga agatctgccc cacaaggccg
    17281 ttggtgagcg gcacggtccc cagccacagc aggccgatgc cgcacgcgaa cacatacaag
    17341 gtcaggggcg aaagcggaaa gaagacgaga agcgcgatca gcacgccgcg caccgaatag
    17401 atgctcgaca ggatgtactt cttgctgaag cgcccgccga gccagcccgc gctcagcgta
    17461 ccgatgatgt tgaagaggcc aatgagcgcg agcgccgtca tgccgtgcgt tgcgctcagg
    17521 ccggcgtcgg tgacgaagga cggcaggtgc accgtcagca tggcgatgtg gaagccgcac
    17581 acgaagtagc cccagaacag caggtggaag ccccgctcgc gcatggcttc gcgcagcgca
    17641 gcggtgagcg actgccccgt tcccggagca gcggccggcc ggctggacaa agcggccgcg
    17701 agcggcatga tgagcgccgt catccccgcc atgatgagca gcgcgacatg ccagttgaag
    17761 gcgccgatca ggagctggtt gacgggaagc agcgcgaact ggccgaacga ccctgccgcg
    17821 gtggcaacgc cgagcgccgt gctgcgcctt tcgggggcca cggtgcggcc gatgaccgcg
    17881 agcaccgtcg cgaaagtcgc gccggcaagc gcggcgccga ccagcaggcc ggccgcgaaa
    17941 tgcatcgcgg tcggcgtctg cgcgaacgcc atgccgatca ggccaagcgt atagagcgcg
    18001 ccgcaggccg cgaccacgcg gccggtgccg tagcggtcgg cgacgatgcc ggcaagcggc
    18061 ccgagcgcgc cccacacgat ctgctggagc gcgagcgaga acgaaagcac ctcacggccc
    18121 cagccgaact cgaggctgat cggctgcagg tagaggccga aaccggcgcg cgcgccgaac
    18181 gccaggaaca ggatcatgca gccgcagacg agcaccgtgc cgggattcat ggcgcccagt
    18241 ctaccggctc gcgacgcatg ccgcgccggc acagttgcca tgaaaaaggg tcatcccagg
    18301 aagtagcgcg ggatccaggt cgaaaggccg gggacgaaaa tcaccaccac cagcgcaagg
    18361 cactccgccg cgaagaacgg ccacacgtgc cgggtcgtgt cgtcgagcga ggtcttggcg
    18421 atgttcgtgg tcacgaagag cagcggcgcc gtgggcggcg acaccgcgcc gatctggttg
    18481 cagatcacca tcaccaggcc catctggatc ggatcgatgc catagcgctt gcacagctcc
    18541 accgcgaccg gcgcgaacac cagcaggatc gcgagcgaat cgacgaaggt gccgaggagg
    18601 atcatcacca gggcgaagac cagcagcacg acgtaggcgt tcttcgacgc cgtcgtgacc
    18661 catgccaccg ccatgtcgtt gaattgcatg taggcgagca gccagcccat cgcgccggaa
    18721 atcgcgatca ccgccgacac catggtggtg gtgatggccg cctccacaaa aatgtccctt
    18781 aaatctccta agcgaatctt tcggtaccag aacgcgccga cgaggtatgc gtaaagacac
    18841 gccacgacgc ctgcctcggt cgccgtaaag atcccgccga gaatgccgcc cacgatgacc
    18901 aacggcccga gcagcgcggg ccataccctt ttcaatgaag agaggacctt ctggaactcg
    18961 aaagaaccgt gcaccgcgcg cagctcgggg tacgccgggt ggtaggtgta gatcttcacc
    19021 acgcccatca ggaaaaatcc gatcagcagg ccgggcagga tgccgccgag gaacagtccg
    19081 ccgatggaga cttgcgcgat ggcgccgtac accaccatcg tcatgctcgg cgggatgatc
    19141 gcgccgatcg tgcccgcagt ggcgatgagc gcggcggcga agccggcctt gtagccgttc
    19201 tgcttcatcg tcggcaccag cacctgggcg atggcggagg cgtcggcggt ggaggagccc
    19261 gacacgccgg agaggatcat cgagcagacg accgaggcgt gggccagccc ggccttcagg
    19321 tgaccgacca gcacgcgcga gaaatcgacc agctgctcgc tcatgccgcc ggccgtcatg
    19381 agcgcgccgg cgaggatgaa gaacggcagc gcgagcagcg tgtacgagtc gagctgggcg
    19441 aagctcttct gcgcgaacat cggcatcacc acgccgggct cggcggcgat gccgatgaag
    19501 ccgatcagtg cgatgcacag caggatcggg atgccgatca gcatcgtggc gagcagcagg
    19561 cccagcatca cccagatcat gggtccgggg tttccttcag aaatactctc ctcagtacaa
    19621 cgatgaggag ataaacacct ccgacgacca tgctgtagta gacgtagttc atcggcagct
    19681 cgagccccgg cgagctctgc agcttcgcca cctcggacac gcgccaggcg agccacgcga
    19741 gcgcgaccgc gaaccagatc cacaggatct cgatgaagta gtcgaacgcc tttcccacct
    19801 tcggcggaaa cttattgcgg aaggattcca ccgcgaggtg ggcgccgcgg cggtaggcga
    19861 tcgggatgcc gaggaacacg atccagacgt ggcagtagat ctggaattcc tcgctccagc
    19921 tgagcgagcg gttgagaacg aagcggttga agacctgcag caggccgact gcgcacatcg
    19981 cggcgaagat cgccgcgacg ccgtactcgg cgccgcggtc gatccacgca aaggctcttt
    20041 tcaaggggtt tttctgattt gctccagcag cttctcggcg ccgaactcct tcgccagttc
    20101 gtcctgcgcc gcgagcgcgg ccttgaccag cggcgcggtg tcgatctcgt tcaccttcat
    20161 gcccaggcgc ttcacctcgg cgagcgcctg cccctcgcgc tgcggcgcca tctcgcgcac
    20221 gatcttgccg gcctcgatgg ccaccttctg cagcgccgcc tgctccgggg ccgagagctt
    20281 cttccactcg gcaagcgaga acaccaggaa cgtcgggtcc atcaggtgct tggtgagcgc
    20341 catgcagcac gccacctcgt acatcttgaa cgagatggtg gtcgccgcgg tgtgctcgta
    20401 gccgtcgagc acgccgctct gcagcgaggt gtagatctcg ccgaagttca tcggcgtggc
    20461 gttggcgccc atcgagttga tcgccgcgac gaacaccttg gtcgggatgg tgcggatctt
    20521 gaggcccctc agatcggcgg cggtcttcac ttccttcttc gaggtgatga cgttgcggta
    20581 gccccagctc accgcccacg ccagcacctt gaatcccgcc ttctccatgt cctggtcgag
    20641 ttccttgccg accgggccgt cgagcacctg cagcgcgtga tcgtagctct tccactcgaa
    20701 cggcaggccg agcacgccga ggcgacgcac gaaggaagcg tattccgccg ggccgccggt
    20761 ggtgcccgcc gcgcccgagc ccagccgcat cgcctcgatc acctcgcgcg tctggccgag
    20821 ctggctcgcc gggaacacct tgatttcgac cgtgctctgc gccgcattgg cgcgatcctg
    20881 gaactcgacc gcgagcttgg tgttgtcgac gttggtgtcg ttcggcccgg cggtatggat
    20941 gcgcagcagt ttcttggttt gcgccgtcgc cgtgagcgca aaggtcatta gcaacagcag
    21001 catgaatgtt cgcttcatga gtcccctcct cttgaacctc gttaggatgc gagcttttcc
    21061 acctgcggga gattgcaggc gaggatgctt ttgcgcaaca cctcgatcgc ctcggggcgc
    21121 gggaagctgc gccgccacgc gatcgcaacg cgccgcgtcg gcaccggccg cgcgaacggc
    21181 aggatgcgga tcaggtcgcc cgcgccgccg gcgccggtcg acgtcgcggg cagcacggtg
    21241 acgccgacgc cgccggccac catctggcgg atggtctcga gcgagccgcc ctcgagggtc
    21301 cgggcgagcg ggctgcgctc gcgcgcgcgc acggtgtggc agatctcgag cacctggtcg
    21361 cggaagcaat gcccctcgcc gaggagcagc aggctctcgc tcgtcagctc ttcggaggtc
    21421 acgcgcttgc ggccctccca cggatgcccc ttcggcaccg cgaccaggaa cggctcgtcg
    21481 taaacggcgc gcgtcaggac gcccggctcc tcgaacggca gcgcgaccag gatcacgtcg
    21541 acctcgccgt tcttcagctc gtcggcgagg cgatgggtga agttctcctg aatgaggagc
    21601 tgcatcgcgg gcgcggtgcg cctcaggatc gggatgagct tcggcagcag gtacggaccg
    21661 atggtgtaga tcgcgcccag gcgcagcggc cccgccagcg gatcgcggcc ggcggcggcg
    21721 atctcgcgga tgcgcgaggc ctcctccagc acgcgctgcg cctgctcgat gatcttctgt
    21781 cccgacggcg taacgctgac ctcgcccggc ccgcgctcga acaggatgac gccgagctcc
    21841 tcttccagct tcttcaccgc gaccgaaagc gtcggctgcg aaacgaagca ggtctccgcc
    21901 gcccgcccga agtggcgctc gcgcgccact gcgacgatgt agcggagctc cgtcagcgtc
    21961 atctattccg ggcaaggaag tacagcggaa tgccgagcgc catgatggcc aggccggcga
    22021 acacggcgac gccgaactcg accgcgaagc gcacgtagtt gacgctcgaa tagagcatgt
    22081 agacgcaggc gatgcagaac gcgatcggca ccaccggata aagcggcacg cgaaacgccg
    22141 gcgcttgcgg ttctcttcgc ctgaagacga acagcgtcag cgcggtgagc aggaagaagg
    22201 tccagaacac cggggacgta taggcaacca tcgcgctgaa gccatcgggc gtgaacgtgc
    22261 tcgccagtac cagcaccagc gtgaccgcgc cctgcaggat cagcgcgttg gccggcgtgc
    22321 tgcccgattc acgccaggcg ccgagcttgc cgaagaacgg gaagtcccgg ccgagcgcgt
    22381 aggtggtcct cgcgccggtg aagatcgcgg cgttcatggt ggtcagggcg gcgaggcaga
    22441 cgatcagggc gatcacgatg gcgcccttgt cgcccatcac gagccgcacc acatcggcgg
    22501 cgaccgcctt ggattccttc atcccgccga gcccgagggc cgcgaggtag gcgacgttca
    22561 cgaggaggta gatcaccgtc agcgtgagaa ttccaccgat caggatgcgg atcatgttgc
    22621 gccgcgcgtc gcgcacttcg cccgacaggt acgcggcctc gttccagcca ccgaaggtga
    22681 gcagcacgaa gatcatggcg aagccgaacg agccgccgct gctcgcagcc accgcgggtt
    22741 tcgcgggtgc gccagccatc aggccggcga tcacgaggaa ggcgaggccg gcgaacagga
    22801 ggaactgcat gaccttctgc agccccttcg cctcgaccgt gcccgccacg ttcagcgccg
    22861 tgagcgcgac gacgccgagc gcggcgtaga tcgcggaact gttttttcca aaagagaaaa
    22921 tttctgacgc gtagtcgcca aagacgaagg ccactgcggc gatagcgccg gtctggatca
    22981 cggtcatgcg cgcccaggcg aacacgaacg ccacgccgcg ccccatgccg cgcgacagga
    23041 aagcgtactc gccgccggtc tcgggatggc gcgtggcgag ctcggcatag accagcgcgc
    23101 cgcagagcga gattgccccg cccagcaccc acgccccgag gaactcggcc ccgctcgagg
    23161 tgttgccggc gacgatcgac ggcgccttga agatgccggc gccgatcacc atgccgcaga
    23221 tcaggatgat gccgtcccac accgtcagca attgcttcgg tgctaccgtg ctctgcttgc
    23281 cgtccaaggg aagctcctct tgttgttgcc gtcggaaacc gtgacttcgc cggtgatgcg
    23341 gtcgtccctg agggtgccga cgaagtagtg atgccaggcg cgcccgttga cgacgccggt
    23401 gacgatcacc ttgatctcct cgccgcgcag ccggctgttg cgcaccagca ggtcccggcc
    23461 ctgcgctccg acctcgatgt cgagcacctg ataggtttgc ttgatcctga agttccagcg
    23521 cccgccgtgc gcgggcagat cgctggccca gctacccttg gcgtcggcgg gcacgatcca
    23581 cagctcgacg cgcgacacgc cgccgacgcc gaccggcttc tccggcgcgc gcatctcgag
    23641 gcgctcgtct ggcgcccagt cgccgatgcc gccatcgtgc gacacgatgc gcgtgccggg
    23701 cttgagcttg agcagcttgg gaagcagctt ggcgttgaac tcgggcagca ggtagaacgc
    23761 gaccaccgtg gcgccggaaa gatcggtctc gaagatatcg cgcacctcga agcgcgcgcg
    23821 ctcgccgacg ccggcgccgt tcgcgttctc ggtcgcgagc ttcaccaggc tgggatcgag
    23881 gtccacgccc aggccgcgcg cgccgcgctt cgccgcctcg atcacgatgc ggccgtcgcc
    23941 cgagccgaga tcgatcaggt agtcgccgcg cccgacttcc gccatgcgca tcatgcgctc
    24001 gacgatttcg gccggcgtct gcacataggg cacggtgccg tcgtcccagg cccaggtttg
    24061 gggttgtgcg accgcaggcg cggccaggaa taaaagggga aggagaaacc gggcgacgtg
    24121 cctatcgagc atgccggaat catataggcc gcctaagacc cttttgtgcg cgggaagtaa
    24181 aatccccgcc gcgccccggt agctcagtgg atagagcggc cccctcctaa ggggtaggtc
    24241 ggacgttcga ttcgtctctg gggcgccacc aactgctcgg gctctgaacc cgatttacct
    24301 gagcttgcgc accgcccaat cacgcgcgcg ccgaatcccg cgcag
//
LOCUS       DATBSZ010000089         3283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1379387, whole genome shotgun sequence.
ACCESSION   DATBSZ010000089 DATBSZ010000000
VERSION     DATBSZ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379387"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VIG70_05940"
     CDS             complement(<1..900)
                     /locus_tag="VIG70_05940"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY8250128.1"
                     /translation="MTIKAAALQNAILTSASFSIIATDERGVIQLFNVGAERMLGYRA
                     AEVVDRISPSDIHDPAEVTARARALSLELGTPIAPGFEALAFKASREIEDSYELTYIR
                     KDGSRFPAVVSITALRDDDARIIGYLLIGTDNSVRKLVESRLNEAMAAAENANRAKTD
                     FISSMSHELRTPLNAILGFAQLMDSGTPAPTPSQKRNLEQILKAGWYLLELINEVLDL
                     ALIESGKVTLSREPVSLVEVMRECRAMIEPQAHKRGIAMQFPRFDVPQFISADRMRFK
                     QVLINLLFNAVKYNRPGGAVTVEC"
     gene            complement(909..2099)
                     /gene="gspF"
                     /locus_tag="VIG70_05945"
     CDS             complement(909..2099)
                     /gene="gspF"
                     /locus_tag="VIG70_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEY8250129.1"
                     /translation="MEAFRYQALDAAGRSVSGVVQADTPREARAQLRGRGLLPSEVEK
                     NARHSWARGISAAQLSLLTRQMATLLDSGLTMEQALAALIEEAEAPLTREVLAGVKAE
                     VMAGASLATALGSHARSFREFYRALVHGGEESGALPTVLAHLADYLDARQALRQKTSL
                     ALLYPSLIGVVAIAIVAGLLVYVVPQVVQVFQQSRQALPLLTRALIGLSEFLRSAWPF
                     LLLIVGGIAGGAHMLLRRDAPRRRWHAFLLRLPWLGPLIRGVDTSRFASTLAILVGGG
                     VPLLAALASGARAMGNSVMREAVERAIERVREGTSLARALGETRNFPPLLVHLVASGE
                     MSGKLEQMLERAARLETQALERRLAVFLTLLEPAMILVMGAVVLLIVLAILLPIIGIN
                     QLVH"
     gene            complement(2102..>3283)
                     /gene="gspE"
                     /locus_tag="VIG70_05950"
     CDS             complement(2102..>3283)
                     /gene="gspE"
                     /locus_tag="VIG70_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEY8250130.1"
                     /translation="DVDLSRLLQEIPKVADLLDSRDDAPVIRLINALLTQALRDGASD
                     IHFEAFETRSLVRLRIDGTLRDLVEPARALHGAIVSRLKIMAQLDIAEKRLPQDGRIA
                     LRVAGKPVDVRVSTIPTGHGERVVLRLLDKQAGRLDLAQLGMDDATLAHMDRLIKEPH
                     GIVLVTGPTGSGKTTTLYAALSRLDTAALNIMTVEDPIEYDLDGISQIPINARIDMSF
                     ARALRTILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVFATLHTNDAVSAVTRLVDM
                     GVEPFLLASSLIGVGAQRLVRRLCLECRKPVVQEDKTYYSAVGCAACNRSGYRGRTGI
                     YELLTVDDDLRRLIHDRASEQALRAHVVARGMKSLRDDGMRWAAAGVISLEEAIRVTR
                     E"
BASE COUNT          513 a         1098 c         1150 g          522 t
ORIGIN      
        1 gcactccact gttaccgcac ccccgggccg gttgtacttg acggcgttga acagaaggtt
       61 gatcaggacc tgcttgaagc gcatgcggtc ggcgctgatg aactgcggca catcgaagcg
      121 cgggaactgc atggcgatgc cgcgcttgtg ggcctgcggc tcgatcatcg cgcggcactc
      181 ccgcatcacc tcgaccagcg acaccggttc gcgcgacagc gtcaccttgc ccgactcgat
      241 cagcgcgagg tcgagcactt cgttgatcag ctccagcagg taccagccgg ccttgagtat
      301 ctgctcgagg ttgcgcttct gcgagggcgt gggcgccggc gtacccgagt ccatgagctg
      361 ggcgaagccg aggatcgcgt tgaggggcgt gcgcagctca tggctcatgc tggagatgaa
      421 gtcggttttc gcgcggttgg cgttttccgc ggccgccatg gcctcgttca gcctggattc
      481 gaccagcttg cgcacggagt tgtcggtgcc gatcagcagg tagccgatga tgcgagcgtc
      541 gtcgtcgcgc agcgcggtga tcgacacgac cgccggaaag cggctgccgt ccttgcggat
      601 gtaggtgagc tcgtagctgt cctcgatctc gcgcgaagcc ttgaacgcca gcgcctcgaa
      661 gcccggcgcg atcggcgtgc cgagctcgag gctcaaggcg cgcgcgcgcg ccgtcacctc
      721 cgccgggtcg tggatgtcgc tcgggctgat ccggtcgacg acctcggcgg cgcggtagcc
      781 cagcatgcgc tcggcgccga cattgaagag ctggatgacg cctctctcgt cggtggcgat
      841 gatcgaaaag ctcgcgctgg tgaggatcgc gttctgcaag gctgcggctt tgatggtcat
      901 ttttcctttc agtgcaccag ctggttgatc ccgatgatcg gcagcaggat ggcgagcacg
      961 atcagcagca ccaccgcgcc catcaccagg atcatcgccg gctcgagcag ggtgaggaac
     1021 accgcgaggc gccgctcgag cgcctgggtc tcgaggcgcg cggcgcgctc cagcatctgc
     1081 tcgagcttgc cgctcatctc gccgctcgcc accaggtgca ccagcaacgg cggaaagttg
     1141 cgcgtctcgc cgagcgcgcg cgccaggctg gtgccttcgc gcacgcgctc gatcgcgcgc
     1201 tcgacggcct cgcgcatgac gctgttgccc atcgcccgcg ccccggaggc gagcgccgcg
     1261 agcagcggca cgccgccgcc gaccaggatg gccagcgtgc tggcgaagcg ggaggtgtcg
     1321 acgccgcgaa tcagcgggcc gagccacggc aaacgcagca agaacgcgtg ccaacggcgg
     1381 cgcggtgcat cgcggcgcaa cagcatatga gctccgcctg cgatcccacc aacgatcaaa
     1441 agtagaaaag gccatgccga gcgcaggaac tcgctaagcc cgatgagcgc tcgcgtcagc
     1501 agcggcaggg cttgccgcga ttgctggaac acctgcacca cctggggcac gacatacacc
     1561 agcaggccgg ccacgatggc gatggccacg acgccgatga ggctcgggta gagcagcgcg
     1621 aggctggttt tctgccgcag ggcttggcgt gcgtcgagat agtccgcaag atgggcgagc
     1681 accgtcggca gcgcgcccga ttcttcgccg ccgtgcacga gcgcgcggta gaactcgcga
     1741 aagctgcgcg catggctgcc gagcgcggtg gcgagagagg cgccggccat gacctcggcc
     1801 ttgacgccgg cgagcacttc gcgggtcaag ggtgcctcgg cctcctcgat cagcgccgcg
     1861 agcgcctgct ccatcgtcag gccggagtcg agcagcgtcg ccatctgccg ggtcagcagg
     1921 ctgagctggg cagcggagat gccacgcgcc caggaatgcc gtgcattctt ttccacttca
     1981 gagggcagca ggccgcgtcc gcgcaattgc gcgcgcgcct cacgcggcgt atcggcctgc
     2041 actactccgg aaaccgagcg gcccgcggcg tcgagcgcct ggtagcggaa ggcctccatg
     2101 gttattcccg cgtcacgcgg atcgcttctt ccaggctgat cacgcccgcc gcggcccagc
     2161 gcatgccgtc gtcgcgcaag gacttcatgc cgcgcgcgac gacgtgcgcg cgcagcgcct
     2221 gctcggaggc gcggtcgtgg atcaggcggc gcaggtcgtc atccacggtc agaagctcgt
     2281 agatgccggt gcggccgcgg tagcccgagc ggttgcacgc ggcgcagccc accgcggaat
     2341 aataggtctt gtcctcttgg accacgggtt tgcggcattc caggcacagg cgccgcacca
     2401 ggcgctgcgc gccgacgccg atcaggcttg aggcaagcag gaacggctcg acgcccatgt
     2461 cgaccaggcg cgtgaccgcg ctcaccgcgt cgttggtgtg cagcgtggcg aataccaggt
     2521 gcccggtcag gcttgcctgc accgcgatct gcgccgtctc caggtcgcgg atctcgccga
     2581 tcatcaccac gtcggggtcc tggcgcagga tggtgcgcag cgcgcgcgcg aagctcatgt
     2641 cgattcgcgc gttgatcggg atctggctga tgccgtcgag gtcgtactcg atcgggtctt
     2701 ccaccgtcat gatattgagc gccgcggtat cgagccgcga cagcgcggca tagagcgtgg
     2761 tggtcttgcc cgagccggtc gggccggtga ccagcacgat gccgtgcggc tccttgatca
     2821 gccggtccat atgcgcgagc gtcgcgtcgt ccatgccgag ctgcgcgagg tccaggcggc
     2881 cggcctgctt gtcgagcagg cgcagcacca cgcgctcgcc gtgcccggtg gggatggtcg
     2941 acacgcgcac gtcgaccggc ttgccggcga cgcgcagcgc gatgcggccg tcctgcggca
     3001 ggcgcttctc ggcgatgtcg agctgcgcca tgatcttgag gcgcgacacg atcgcgccgt
     3061 gcagcgcgcg cgccggctcg accaggtcgc gcagcgtgcc gtcgatgcgc aggcgcacca
     3121 gcgagcgggt ctcgaaggcc tcgaaatgga tgtccgaggc gccgtcgcgc agcgcctggg
     3181 tgaggagcgc gttgatcagg cggatcaccg gcgcgtcgtc ccggctgtcg aggagatcgg
     3241 cgaccttcgg gatctcctgc agcagccgcg acagatccac gtc
//
LOCUS       DATBSZ010000090        17540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1242871, whole genome shotgun sequence.
ACCESSION   DATBSZ010000090 DATBSZ010000000
VERSION     DATBSZ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17540
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242871"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VIG70_05955"
     CDS             complement(<1..399)
                     /locus_tag="VIG70_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250131.1"
                     /translation="MHAVAGRSAAGRLYRSQLFRKYFLLILALVCGTLLISGGIGLYF
                     AYQENKEALASLQREKAAAAAARIEQYVLQIEQHLSSVALTQYGAQSLEQRRLEFLKL
                     LRMVPDVTDVAQIDARGREQLSVSRLKMDEL"
     gene            complement(509..688)
                     /locus_tag="VIG70_05960"
     CDS             complement(509..688)
                     /locus_tag="VIG70_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250132.1"
                     /translation="MTKTEHLQFLVLMIPTFLILTAAAVSMADLASPAAQPVYLAAAD
                     PSVPAHLVWDDRPAE"
     gene            832..1467
                     /locus_tag="VIG70_05965"
     CDS             832..1467
                     /locus_tag="VIG70_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8250133.1"
                     /translation="MKDRTVEVLPQPLLDKVAAVATVRTFPKRAILVTEGDDTDSLYV
                     VLSGKVRVFVADDKGREVQLNQLGVGEYFGEVTLDGGPRSASVMAIEDCRCAVVKRAE
                     LTTFLEKQPELSLHIVRKLAHRVRELTENVRSLALMDVYGRVARLLLELAEEKEGKLV
                     IDEALTHKDIASRVGASREMISRIFSDLSDGGYVRKEDGHLVIARKPPPRW"
     gene            1535..1735
                     /locus_tag="VIG70_05970"
     CDS             1535..1735
                     /locus_tag="VIG70_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250134.1"
                     /translation="MADLSDAVVWIVRLVLVVGLGWGAWLCVGHLLLPARSERVLDME
                     HFATFALIVLLLTTLGGFLHAS"
     gene            1851..2060
                     /locus_tag="VIG70_05975"
     CDS             1851..2060
                     /locus_tag="VIG70_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250135.1"
                     /translation="MKSADPSERRWLGRQLANVLAALPAAQDELAAQSTRARLPQMKE
                     LARQVAMLETVVLELACEHRIEELA"
     gene            complement(2191..3279)
                     /locus_tag="VIG70_05980"
     CDS             complement(2191..3279)
                     /locus_tag="VIG70_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8250136.1"
                     /translation="MAERILVVDDQRTNAEMVSGLLRNLGYDVEVALSGDEALEMVRA
                     KAPDLMITDILMPGLDGYEVCRRLRANPATALLPVILVTSAEPQTERVKGIEAGADDF
                     LAKPVNWAELFGRVRRLLHVKALQDEIKELNARLEERVRDQVAQLERLSRMKRFFSRA
                     VAEAIVAPGGEELLEPHRREITAVFLDLRGFTAFTDRADPDEVLDLLRAYHATLGRTV
                     DEFGGTLEHFAGDGVMIFFNDPIEIANPAERAIRMALALQRAFNPIAEAWVKIGHEVG
                     LGIGIAQGETTLGVIGFEQRWEYAAIGNVPNLAARLCGAATAGEIIVDGQTEQDIVAI
                     ADTEFIGALTLRGFQQPVPAFRLKSIRD"
     gene            complement(3294..4400)
                     /locus_tag="VIG70_05985"
     CDS             complement(3294..4400)
                     /locus_tag="VIG70_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEY8250137.1"
                     /translation="MPRKAKAALCRAHNTPVLVESIAVDPPKRGEVTVKLGACGVCHS
                     DLSAITGTIALPLPLVLGHEGAGVVEEVGEGVADLAKGDHVVFSFIYMCGKCRFCVAG
                     RPVLCLEAGKALATPREGTPRVRDAAGAPLNIFSGCGAMAEYATVSAENLIRIDPKIP
                     LDCAALVGCGVTTGVGAVFNTAKVEPGSSVAVFGCGGVGLSVIQGARIAGAERIVAID
                     TLEAKLEMAKKFGATDTLVFGEDVVKPLKKMTGGGPDYAFECVGSGELAATAYRAIRR
                     GGVAVVVGVAKPSDSTAVRTMTLPFEEKVLTGSSFGSCVPRVDFPRMLALYMAGRLKL
                     EELITRRYSIDEAPQAFADLQAGKNARGVIVFGP"
     gene            4427..5854
                     /locus_tag="VIG70_05990"
     CDS             4427..5854
                     /locus_tag="VIG70_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8250138.1"
                     /translation="MLLRDQLFIGGVWVAPSGKQTIDVFNAGNGEKLGRVPLGDEKDV
                     EHAVAAARAAFEGWSGGSAAWRAGFLEKISAGLKVRADELAKLIAQEVGMPLKLSARI
                     QAGLPIANFANYARLVKEFPFESRVGNSVVVREPVGVVGAITPWNYPLHQITLKVAPA
                     LAAGCTVVLKPSEIAPLNAFVLAEVMETAGLPKGVFNLVTGTGQKAGEALVRHPGVDM
                     ISFTGSTRAGKQISKLAAKTVKRVALELGGKSASVILEDADLATAVKGTVNGCYLNSG
                     QTCTALTRMLVPESRYEEAAKLAAEAAKGFTLGDPLAESTRLGPLSSLAQLQRVREYI
                     KKGVQEGAELITGGPELPEGVPAGGYYVRPTVFGKVKNSMTIAQEEIFGPVLSIITYK
                     DEEDAVRIANDSPYGLAGAVWSKDDAHAQRIARRIRAGQVDVNGGAFNLNAPFGGFKQ
                     SGHGREAGVYGLEEFLEYKSLQLKG"
     gene            5851..7521
                     /locus_tag="VIG70_05995"
     CDS             5851..7521
                     /locus_tag="VIG70_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8250139.1"
                     /translation="MRLARLITGLVVASFVVGHFLNHALGVVSIEAMDRMRFMLAVWW
                     RSPVGTFLLYGSLLTHFFLALASLYKRSTLRMPWWEAAQLALGLAIPPLLIVHIVGTR
                     LTWTLLGHNIDYERIVGLLWSSEWSTAKQVLLLLVVWGHVCAGLHYWLRLRQWYRNAL
                     PLGFALALLLPALALAGFASAGFALWPSVESVGGIHKYNVDLAGMTARDRELIAHWRD
                     GLEYGFWIALGATLLARWLRTRIGGTYRLRHASGRVINAPVGRSILEAIRDEGLPHAS
                     VCGGRARCTTCRVRIGDGLAHLPPPGRMEAQALGRIEAPTNVRLACQCRPTRDVTVTP
                     LLPPNAGPSSALQREGKTPGGALGRERPIVAMFVDLRDSTHLAEGRLPYDVVFIMNQF
                     FAEMHAALRATGGYYAQFRGDGLLALYGLESSLASACRAALEGAAEMQTRIVRLNQSL
                     ADELEEPLRIGIGIHAGVAIVGTMGPPDAPIYSAIGDMVNTAARLENMTKALGCVLVV
                     SAEALRQAGTDPGSAREHQVRIRGKTERLTVYAVDDSRKLIQGQTTVS"
     gene            complement(7518..7979)
                     /locus_tag="VIG70_06000"
     CDS             complement(7518..7979)
                     /locus_tag="VIG70_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8250140.1"
                     /translation="MSLEQEVELIRQFPIFAKIQPAMQKLLCFSAERLTYAPGQVMFK
                     VGDMGDAAYIVIEGSVQISVPSPSGPIIVNTLGRNDIIGEIAIFGDVPRTATATAQTK
                     LETLKISKELFEKIIRENPDAALELIRVLAARLANTTAQLSRTMSGTRPGA"
     gene            complement(7976..10609)
                     /locus_tag="VIG70_06005"
     CDS             complement(7976..10609)
                     /locus_tag="VIG70_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY8250141.1"
                     /translation="MERSLFGFIIRYSKRDQLRIVPFVLLTMVVYYASLDLPKTIINE
                     AIQGGRFTDGSSSAGFFGLELGRLEYLFALSMIFLGLIVLNGWLKFRINTMKGWMGER
                     MLRRLRYVLYDHILRFPLSRFRRLKAAEMATMIKDEVEPLGGFIGEALITPLFLGGQA
                     ATAMFFILYQHWALGLIALAVVGVQAYFIPRLRKRLIVLGRERQLTARALAGRIAETV
                     DGAAEIHANATSNYERAEISARLGRIFHIRFEFYQRKFLIKFLNNFLSQVTPFLFYTV
                     GGYLVIMGSLDIGALVAVIAAYKDLPGPVKELIDWDQQRLDVEVKYEQVMEQFLDEEV
                     APPEMQALLESPQLPKGGKLRAANLSLVDEHGTRLVESVSFEAGLDQHVAIVGRPGSG
                     ASELAQLLARLQQPTSGSFELGGVDMTRAPEAITGRAIGYVGSPAYLFPARVRDNVLY
                     GLKHRLVQEKTYDKEAATQREFQAREAARTGNSTLDIEADWIDYEAAGCTGAEDCDKR
                     IVELLRAVDLEETIFELGLRSRAQKHAGSDVGARVLEARRHMRERLESLGISDWVERF
                     DPAAYNRNATLAENLLFGTPLGRVFDMENLAGNAYVRQVLRATGLDELLLRTGHKVAE
                     TMVELFSGLPAGHEFFGQYSFINQDDLPQFEAILKRVGEVGLKGLAAEDTRALLALTF
                     MLIATRHRLGLIDEGFEGRVIEARRYFAENLPPELRDAVEFFDPARYNDAATLQDNIL
                     FGKIVTGQAGATERITKLVRELLEELGLRPMVVRNGLDYQVGVGGSRLPVADRQKVAM
                     VRAMLKRPALLVLDQAAAVLDPASQLRVVEGVLRERKDQAVAWVLSRPEYADRFGVVL
                     VMERGRLVEKGKFAELKRKTG"
     gene            10653..11489
                     /locus_tag="VIG70_06010"
     CDS             10653..11489
                     /locus_tag="VIG70_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8250142.1"
                     /translation="MTFSVRFWGVRGSIACSGPRTARYGGNTSSLEVRCGERMLLFDA
                     GTGLRYLGNSLSSAQPASQPVDADLFLTHTHFDHVCGLPFFRPFFQPQNRFRLWAGHL
                     AEGMTLRRVLGEFMMAPLFPVPPQVFRARMEYREFKAGETLRPAEGVAVRTATLNHPD
                     GATGYRVEYGGRSVCYLTDTEHVPGAPDRHILELIAGADLVIYDSMYTDAEYDTYVGW
                     GHSTWQEGVRLCRAAGAKRLAVFHHDPEHDDDMLDGVAREVARELPGSIVARDGLVVE
                     LN"
     gene            11476..12153
                     /locus_tag="VIG70_06015"
     CDS             11476..12153
                     /locus_tag="VIG70_06015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8250143.1"
                     /translation="MSLIERYAAELEAIGAEPAPAPRWDQDWFPRLDAAAAYAMVRST
                     RPRRIVEVGSGHSTRFLARAVADGGLDTRITAIDPKPRAAIRGLNVEWLQARVETLDH
                     GPFARLGENDILFIDSSHQLRPGSDVEFLVNQVIPRLPAGVHVHFHDIFLPDDYPVRW
                     AWRRYNEQAAVASLIENNLFRIDFSSHVTVSRSPEKIAGVLARLPLVPGAVESSLWLT
                     KLSTSSR"
     gene            12120..13280
                     /locus_tag="VIG70_06020"
     CDS             12120..13280
                     /locus_tag="VIG70_06020"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8250144.1"
                     /translation="MADEALDIQPVIDWLVEGARPARRPQDVLVDTCQRTVSAGLPLY
                     RVGVFVRTLHPNVLGRAFIWQADTGAVEISEAAHDLLESETFLKSPIRVVFTEHVEVR
                     RRLADPACPMDFPILEDLRKEGATDFVAAPLRFVNGEVHAASFATRRAGGFRDAELAA
                     LRRLLAPFTRMVEIYGNMQKARNILDAYLGRGAGEKVLAGQIKRGDGQDIDAVIWFCD
                     LRDSAALADSMSRREFLALLNEYFECVLGPVLERQGEVLRFIGDAALAIFPVNGRPAE
                     ACAKALAAAQEAFARMDKLNKKRHSPLRFGIGLHLGELTYGNVGTPSRIEFTVVGAAA
                     NEAARIESLCKQLGVDLLVSERVARALPGPWKSLGSHTLRGVGDKMELFTLS"
     gene            13303..14991
                     /locus_tag="VIG70_06025"
     CDS             13303..14991
                     /locus_tag="VIG70_06025"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8250145.1"
                     /translation="MTALGGLFADWYWRMDGQLRLTYTSHQFAEKTGLDPADDYWEHN
                     RRQLERHQGFRDFEIRRIASDERCVWLALSAEPVFDESGTFQGYHGVGRNITAHKRGE
                     QVLRLEHAVARALAQSPGVAEGLQAGLRAMCDIEGWDYGRCFRVDPASAEITFEEGWF
                     AREPATEQFLSRARATWQEGKPVFAKSPRDSSGSFATFAFPAVAQGKTIGLLVFSGHR
                     SGDPDERLLEAAQAIGSLFGQFLQRKQTEELLRASEARFRSLTHLSSDFFWETDAQHR
                     LTSIVHGPSYGSPQVARGIIGKTPWGVPSASPDQAGWAAHMAVLNSHLPFRDFEFARV
                     MPDGGTRYLSVGGEPRFDAMGVFLGYRGIGRDVTEISLARERIASLAYNDALTGLANR
                     TSLSPALEQAIERTRRHRSKLAGVFMDLDGFKQINDLHGHAAGDAFLVEVARRLRRSL
                     RASDVVARLGGDEFFVVLEQMQDAPAVERVVSKLASEVLKPYELFGGVKTRISASLGV
                     SIFPDDAGDAATLMAHADKAMYAAKRAGKNAFCIFSSGTSGSPTSIPSSDHRAA"
     gene            15058..15270
                     /locus_tag="VIG70_06030"
     CDS             15058..15270
                     /locus_tag="VIG70_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250146.1"
                     /translation="MGPEDNPEDTYRAALVRAAHLLGGARRLGERLQVPMPELTRWLA
                     GDGKPSIGTFLKVIDVLIEESGKPAE"
     gene            15319..15573
                     /locus_tag="VIG70_06035"
     CDS             15319..15573
                     /locus_tag="VIG70_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY8250147.1"
                     /translation="MKTLTVRLPESLAASIEAESRARKLSKSDVVRERLERAPAGAAE
                     PNPASYDIADLIGSVDGLPADLSARKKHYLRLGYGRKRSR"
     gene            15551..15961
                     /locus_tag="VIG70_06040"
     CDS             15551..15961
                     /locus_tag="VIG70_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8250148.1"
                     /translation="MAASVLVDASFVVALFNRRDSNHRWAAAQGSNHARPWHTCEAAL
                     SEAFYLLGPRGAPALRTFLRRGSLRLAFDLDGQLDPVLQLIEKYASVPMSLADACLVR
                     MTETLSNPLLLTIDSDFRIYRRHSRQVVPCVMPD"
     gene            complement(16103..16249)
                     /locus_tag="VIG70_06045"
     CDS             complement(16103..16249)
                     /locus_tag="VIG70_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250149.1"
                     /translation="MRNGIPSVWEHRQFTDVGGLLSYGPDFREDYCIAARYVDRILKG
                     AKAA"
     gene            16302..16430
                     /locus_tag="VIG70_06050"
     CDS             16302..16430
                     /locus_tag="VIG70_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250150.1"
                     /translation="MISASDVFAAANVASNSSGKDATRTCSSIPSERAELVHGVGE"
     gene            complement(16639..>17540)
                     /locus_tag="VIG70_06055"
     CDS             complement(16639..>17540)
                     /locus_tag="VIG70_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8250151.1"
                     /translation="FHDGSFVGPFVYGYSMRLNMQTLKREYTDDRSAVFRLRFFCLGD
                     DYEFWSLIPGRFHFVCPAERGTLYLLGTDRLGRDLFSRIVYGTRISLTVGLFGIAVSF
                     LIGITLGGISGFYGGWIDNVIQRFIEMVRSFPELPLWMALSAALPVNWSPILVYFGIT
                     IILGLLDWPGLARAVRSKLLALREEDFATAAVLMGASPARVIRKHLLPSFTSHLIASA
                     TLSVPAMILGETALSFLGLGLRPPVTSWGVLLNEAQNINVVALYPWLMLSVVPVFIIV
                     LAFNFFGDGLRDAADPYSTTART"
BASE COUNT         2827 a         5988 c         5911 g         2814 t
ORIGIN      
        1 gagctcgtcc atcttcaggc gcgacaccga gagctgctcg cgcccccgcg cgtcgatctg
       61 cgcgacgtcg gtgacgtccg gcaccatgcg caggagcttc aggaactcga ggcggcgctg
      121 ctcgagcgac tgggcgccgt attgcgtcag cgccaccgac gagaggtgct gctcgatctg
      181 cagcacgtac tgctcgatgc gggccgccgc tgccgccgcc ttctcgcgct gcaggctcgc
      241 cagcgcttcc ttgttttcct ggtaggcgaa gtagaggccg atcccgcccg agatcagcag
      301 cgtgccgcac acgagggcga ggatcagcag gaaatacttg cggaagagct ggctgcggta
      361 caggcgcccg gccgcggaac gccccgcgac cgcgtgcatt tcgacgatcg gctctgcttt
      421 caggccctcc tccccgagaa atgcccctta acggggcagt atggccctcc ctcgagaaag
      481 tttcatctgg ccgtccaggg gtggattgct attcggccgg ccggtcgtcc cagaccaggt
      541 gggcggggac gctcgggtcg gcggcagcca ggtagaccgg ctgcgcggcc ggggaggcga
      601 ggtccgccat cgacaccgcg gcggcggtga ggatcaggaa ggtcgggatc atcagtacca
      661 ggaactgcag atgctcggtc ttggtcatgt tccaactccc ctaagagtta tagcgacact
      721 agaccttgtc ccggcctccg gtcagcgaag aacggcgcct cctggtagtg ctgtattcac
      781 gttcgtaagg caaaatgcac caaacatcac tagatcggtt ggaggaggag gatgaaggac
      841 cggacggtag aggtgctgcc gcagccgctg ctcgacaagg tcgcggcagt ggctacggtc
      901 cgcaccttcc ccaagcgcgc gatcctagtc accgaggggg acgacaccga ctccctgtac
      961 gtggtgctgt cgggcaaggt gcgggtattc gtcgccgacg acaagggccg cgaggtgcag
     1021 ctgaatcagc tcggggtcgg cgagtacttc ggcgaggtaa cgctcgacgg cggcccgcgc
     1081 tcggcctcgg tgatggcgat cgaggactgc cgctgcgcgg tggtcaagcg cgccgagctc
     1141 acgacgttcc tcgagaagca gcccgagctc tcgctgcata tcgtgcgcaa gctcgcgcac
     1201 cgggtgcgcg agctgaccga aaacgtgcgc agcctggcgc tgatggatgt ctacgggcgc
     1261 gtcgcccggc tgctgctcga gctcgccgag gagaaggaag gcaagctggt gatcgacgag
     1321 gccctcacgc acaaggacat cgcgtcgcgg gtgggcgcct cgcgcgagat gatcagccgc
     1381 atcttctccg acctctccga cggcggctac gtgcgcaagg aggatgggca cctggtgatc
     1441 gcgcgcaagc ccccgccgcg ctggtgaatc cgtcacaggg gacgagcgca aacggtcatt
     1501 gaggggctgc aggccgccgc gtagggtttg cggcatggcg gacctgtcgg acgcggtggt
     1561 ttggatcgtg cggctcgtgc tggtcgtcgg cctgggctgg ggggcgtggc tgtgcgtagg
     1621 ccacctgctc ctgccggcga ggtcggaaag ggtgctcgac atggagcact tcgccacctt
     1681 cgcgctgatc gtcctgctcc tcaccacact cgggggcttt ctccatgcca gctgatcccg
     1741 ccgtctggcc ggtcgccgag gcgactagcc gatccatcgc cgaggcgact agccgatcca
     1801 tcgacgtctc cctgagccgc atccgcgatt gcgcgggcct agcctcgctc atgaagagcg
     1861 ccgacccgag cgagcggcgc tggctcggcc ggcagctcgc caacgtgctc gccgccctgc
     1921 ccgcggcgca ggacgagctt gccgcgcaat cgacgcgcgc gcgcctgccg cagatgaagg
     1981 agctcgcacg ccaggtcgcg atgctggaaa ccgtggtgct cgagctcgcc tgcgagcacc
     2041 gcatcgagga gctcgcctag ctcgctaggt ggggcaccct gtgcgaagaa tgctcgaggc
     2101 tcacggagtt cgacgctagc caggtgaaga aaagcgggac cgcgggctga gcccgcatta
     2161 gggctccgtc cccgaattca atccccgaat tcaatccctt atcgacttca gccggaacgc
     2221 gggcaccggc tgctggaagc cgcgcagcgt gagcgcgccg atgaactcgg tgtcggcgat
     2281 ggcgacgatg tcctgctcgg tctggccgtc gacgatgatc tcgccggcgg tcgctgcgcc
     2341 gcacagccgc gccgccaggt tcggcacgtt gccgatcgcc gcgtattccc agcgctgctc
     2401 gaagccgatc acgccgagcg tggtctcgcc ctgggcgatg ccgatgccga gaccgacctc
     2461 gtggccgatc ttgacccagg cctcggcgat cggattgaac gcgcgctgca gcgcgagcgc
     2521 catgcggata gcgcgctcgg cgggattggc gatctcgatc gggtcgttga agaagatcat
     2581 gacgccgtcg ccggcgaagt gctcgagcgt gccgccgaac tcgtcgaccg tgcggccgag
     2641 cgtcgcgtgg taggcgcgca agagatccag cacctcgtcg ggatcggcgc gatcggtgaa
     2701 cgcggtgaag ccgcgcagat ccaggaacac ggcggtgatc tcgcgccggt gcggctcgag
     2761 cagctcttcg ccgcccggcg cgacgatcgc ctcggcgaca gcgcgggaga agaagcgctt
     2821 catgcgcgac aggcgctcga gctgcgcgac ctggtcgcgc acgcgctcct ccagcctggc
     2881 attcagctcc ttgatctcgt cctggagcgc cttgacgtgc agcaagcgcc tcacgcgccc
     2941 gaacagctcc gcccagttga ccggcttggc gagaaagtcg tcggcgccgg cctcgatgcc
     3001 cttgacgcgc tcggtctgcg gctcggccga ggtgaccagg atcaccggca ggagcgcggt
     3061 ggccgggttc gcgcgcaggc gccggcagac ctcgtagccg tccaagcccg gcatcaggat
     3121 gtcggtaatc atcaggtccg gcgccttggc gcgcaccatc tcgagcgctt cgtcgccgct
     3181 cagcgcgacc tcgacgtcgt agccgaggtt gcgcagcagt cccgacacca tctcggcatt
     3241 ggtgcgctgg tcgtccacca ccaggatgcg ctcggccatg gaaagtggaa cggctatggc
     3301 ccgaacacga tcacgccgcg cgcattcttt ccagcctgca ggtcggcgaa cgcctgcggc
     3361 gcttcgtcga tcgaatagcg ccgcgtgatc agctcctcga gcttgagccg cccggccatg
     3421 tagagcgcga gcatgcgcgg gaaatcgacg cgtggcacac aggagccgaa cgagctgccg
     3481 gtgagcacct tttcctcgaa cggcagcgtc atggtgcgca ccgcggtcga gtcgctcggc
     3541 ttcgccacgc ccaccacgac tgcgacgccg ccgcggcgga tggcgcggta ggccgtcgcg
     3601 gcgagctcgc ccgagccgac gcactcgaag gcatagtcgg gtccgccgcc ggtcatcttc
     3661 ttcaggggtt tgacgacatc ctcgccaaaa accagggtgt ccgtggcgcc gaatttcttc
     3721 gccatctcca gcttcgcctc gagcgtgtcg atggcgacga tgcgttcggc accggcgatg
     3781 cgcgcgccct ggatcacgga aagcccgacc ccgccgcagc cgaacaccgc caccgacgag
     3841 cccggctcga ccttggcggt gttgaacacg gcgccgacgc cggtggtgac gccgcagccg
     3901 acgagcgccg cgcagtccag cggaatcttc ggatcgatcc ggatcaggtt ctcggccgac
     3961 acggtggcgt actcggccat cgcgccacag ccggaaaaaa tgttcagcgg agcgcccgcg
     4021 gcgtcgcgca cgcgcggcgt gccttcgcgc ggcgtggcga gcgccttgcc cgcttcgagg
     4081 cacagcaccg gccggcccgc gacgcagaag cggcacttgc cgcacatgta gatgaacgag
     4141 aacacgacat ggtcgccctt ggccaggtcg gctacgccct cgcccacctc ttcgaccacg
     4201 cccgcgccct cgtggccgag caccagcggc agcggcagcg cgatggtgcc ggtgatcgcc
     4261 gacagatcgg aatggcagac gccgcaggcg cccagcttca ccgtcacctc gccgcgcttg
     4321 ggcggatcga ccgcgatcga ctcgacgagg accggcgtgt tgtgcgcgcg gcagagcgcc
     4381 gctttggcct tccggggcat cgcttgagta tagctagaat atcgcaatgc tcctccgaga
     4441 ccagcttttc atcggcggtg tgtgggtcgc accgtccggc aagcagacga tcgatgtgtt
     4501 caacgcgggc aacggcgaga agttgggcag agtgccgctc ggcgacgaga aggatgtcga
     4561 gcatgcggtc gcggcagcgc gcgccgcttt cgagggatgg agcggcggct cggccgcatg
     4621 gcgcgccgga tttctcgaga aaatatcagc cggcctgaag gtgcgcgccg acgagctggc
     4681 caagctcatc gcgcaggaag tgggcatgcc gctcaagctc tccgcgcgca tccaggccgg
     4741 gctgccgatc gcgaatttcg cgaattacgc aaggctggtg aaggagtttc ccttcgagag
     4801 ccgggtcgga aattcggtgg tggtgcgcga gccggtgggg gtggtgggcg cgatcacgcc
     4861 gtggaactat ccgctgcacc agatcacgct caaggtcgcg ccggcgctcg ccgcgggctg
     4921 cacggtggtg ctcaagccct ccgagatcgc gccgctgaac gcgttcgtgc tcgccgaggt
     4981 gatggaaacc gcgggcctgc cgaaaggggt gttcaacctc gtcaccggca ccgggcagaa
     5041 ggcgggcgaa gcgctggtgc ggcatcccgg cgtcgacatg atctcgttca ccggctcgac
     5101 gcgcgccggc aagcagatct cgaagctcgc ggccaagacc gtgaagcgcg tcgcgctgga
     5161 gctcggcggc aagagcgcct cggtgatcct cgaggacgcc gatctcgcga ccgccgtgaa
     5221 gggcaccgtc aatggctgtt acctgaattc aggacagacc tgcaccgcgc tcacccggat
     5281 gctggtgccc gaatccaggt acgaagaggc cgccaagctg gcggcggaag cggcgaaagg
     5341 cttcaccctg ggcgacccgc tggcggaatc cacccggctg gggccgctca gctccctcgc
     5401 tcagctgcag cgcgtgcgcg agtacatcaa gaaaggcgtg caggaaggcg cagagctcat
     5461 caccggcggt cccgagctgc ccgaaggcgt gcccgcgggc ggctactacg tgcggcccac
     5521 cgtattcggc aaggtaaaga acagcatgac catcgcccag gaggagatct tcggcccggt
     5581 gctctcgatc atcacgtaca aggatgagga ggacgcggtg cgcatcgcca acgacagccc
     5641 ctacggcctc gccggcgcgg tgtggtcgaa ggacgacgcg cacgcccagc gcatcgcccg
     5701 gcgcatccgg gccggccagg tcgacgtcaa cggcggggcc ttcaacctga atgcgccctt
     5761 cggcggcttc aagcagtccg ggcacggacg cgaagccggc gtgtacggtc tcgaggaatt
     5821 tctcgaatat aagtccctcc aactgaaggg atgagactcg cacgcctgat tacggggctg
     5881 gtggtcgcca gcttcgtggt cgggcacttc ctcaaccacg ccctcggcgt ggtctcgatc
     5941 gaggcgatgg accgcatgcg gttcatgctc gccgtgtggt ggcgcagccc ggtgggcacg
     6001 ttcctcctat acggctcgct tctaacgcat ttttttctgg ctttggcttc cctctacaaa
     6061 cgaagcacgt tgcgtatgcc atggtgggaa gcggcgcagc tcgccctcgg cctggccatc
     6121 ccgccgctcc tcatcgtgca catcgtcggc acgcgcctca cctggacgct cctcggccac
     6181 aacatcgact acgagcgtat cgtcgggctg ctttggtcga gcgagtggtc gacggccaag
     6241 caggtgctgt tgctcctcgt cgtgtggggg catgtctgcg ccggcctgca ctactggctg
     6301 cggctcaggc agtggtaccg gaacgcccta ccgctcggct tcgcgctggc gctgctcctg
     6361 ccggcgctcg ccctcgccgg ctttgcgagc gcggggttcg ccctctggcc gagcgtcgag
     6421 agcgtgggcg gaatccacaa gtacaacgtc gacctcgccg gcatgacggc gcgcgaccgc
     6481 gagttgatcg cccattggcg cgacggcctc gagtatgggt tctggatcgc gctcggcgcc
     6541 acgctgctcg cgcgctggct gcgcacgcgc atcggcggca cctatcggct gcgtcacgcg
     6601 tccggccgcg tgatcaacgc gccggtcggg cgcagcatcc tcgaggcgat ccgcgacgag
     6661 ggactgccgc acgcgtcggt ctgcggcggc cgcgcgcgct gcacgacctg ccgggtgcgc
     6721 atcggcgacg gcctcgcgca cctgccgccg cccgggcgca tggaagcgca ggcgctcggc
     6781 cgcatcgagg cgccaacgaa cgtgcgcctc gcgtgccagt gccgcccgac gcgcgacgtc
     6841 acggtgacgc cgctcctgcc gccgaacgcg gggccttcgt cggcgctgca gcgcgagggc
     6901 aagacgccgg gcggcgcgct cgggcgcgag cgcccgatcg tcgcgatgtt cgtcgacctg
     6961 cgcgattcga cccacctggc cgaagggcgc ctgccgtacg acgtggtgtt catcatgaac
     7021 cagttcttcg ccgagatgca cgccgcgctg cgcgcgaccg gcggctatta cgcccagttc
     7081 cgcggtgacg gtctcctcgc cctctatggt ctcgagtcgt cactcgcgag tgcatgccgc
     7141 gccgccctcg agggcgcggc cgagatgcag acacgcatcg tgcggctcaa ccagagcctc
     7201 gccgacgagc tggaggagcc gctgcgcatc ggcatcggca tccacgccgg cgtcgccatc
     7261 gtcggcacca tgggaccgcc cgatgcgccg atctactccg cgatcggcga tatggtcaac
     7321 acggccgcgc gcctggaaaa catgaccaag gcgttgggct gcgtgctggt ggtttccgcc
     7381 gaagcgctgc gtcaggccgg caccgatccc ggcagcgcgc gcgagcacca ggtgcggata
     7441 cgaggcaaga ccgagcggct gaccgtctac gcggttgacg attcccggaa gctgattcag
     7501 ggacagacca cggtttccta ggctcccggg cgggtgccgc tcatggtgcg cgaaagctga
     7561 gcggtcgtgt tggcgaggcg cgcggcgagc acgcggatca gctcgagcgc agcgtcgggg
     7621 ttctcgcgga tgatcttctc gaacagctcc ttcgagatct tgagggtctc gagcttggtc
     7681 tgcgcggtcg cggtggcagt gcgcggcaca tcgccgaaga tcgcgatctc gccgatgatg
     7741 tcgttgcgcc cgagggtgtt cacgatgatc gggcccgacg ggctcggcac gctgatctgc
     7801 acgctgccct cgatcacgat gtaggccgcg tcgcccatgt cgcccacctt gaacatcacc
     7861 tgcccgggcg cgtaggtcag ccgctcggcg ctgaagcaca ggagcttctg catcgccggc
     7921 tggatcttgg cgaagatcgg gaattgtctg atcagctcga cttcctgttc caggctcatc
     7981 cggttttcct tttcagctcg gcgaacttgc ctttctcgac cagccgtccg cgctccatca
     8041 ccagcaccac gccgaagcgg tcggcgtatt ccgggcgcga gagcacccag gccaccgcct
     8101 ggtccttgcg ctcgcgcagc acgccctcga ccacgcgcag ctgggacgcc gggtcgagca
     8161 ccgccgccgc ctggtcgagc accagcagcg cggggcgctt gagcatcgcg cgcaccatcg
     8221 cgactttctg gcggtcggcc acgggcaagc gcgagccgcc cacgccgacc tggtagtcga
     8281 gcccgttgcg caccaccatc ggccgcagcc cgagttcttc gagcagctcg cgcacgagct
     8341 tggtgatgcg ctcggtcgcc ccggcctggc cggtgacgat cttgccgaac aggatgttgt
     8401 cctgcagcgt ggcggcgtcg ttgtagcgcg ccgggtcgaa gaactccacc gcgtcgcgca
     8461 gctccggcgg caggttctcg gcgaaatagc ggcgcgcctc gatgacgcgc ccttcgaatc
     8521 cctcgtcgat cagccccagg cggtgccggg tggcgatcag catgaaggtc agcgcgagca
     8581 gcgcccgggt gtcctccgcc gccaggccct tcagaccgac ctcgcccacg cgtttgagga
     8641 tcgcctcgaa ctgcggcaga tcgtcctggt tgatgaagct gtactggccg aagaactcgt
     8701 ggccggccgg caggccgctg aagagttcca ccatggtttc cgccaccttg tggccggtgc
     8761 gcaggagcag ctcgtcgagg ccggtggcgc gcagcacctg gcgcacgtag gcattgccgg
     8821 cgaggttctc catgtcgaac actcggccga gcggcgtgcc gaacagcagg ttctcggcga
     8881 gcgtggcgtt gcggttgtac gcggccggat cgaagcgctc gacccagtcg ctgatgccga
     8941 gcgactcgag gcgctcgcgc atgtgccgac gcgcttcgag cacgcgcgcg ccgacgtcgc
     9001 ttccggcgtg cttctgggcg cggctgcgca aaccgagctc gaagatcgtc tcctcgaggt
     9061 cgacggcgcg cagcagctcg acgatgcgct tatcgcagtc ttcggcccca gtgcatcccg
     9121 cagcctcgta atcgatccag tccgcctcga tgtcgagcgt gctgttgccg gtgcgggccg
     9181 cctcgcgcgc ctggaactcg cgctgcgtgg cggcctcttt gtcgtaggtc ttttcctgga
     9241 cgaggcggtg cttcagcccg tagagcacgt tgtcgcgcac ccgcgccggg aagaggtacg
     9301 cgggcgagcc gacgtagccg atggcgcggc cggtgatcgc ctccggcgcg cgcgtcatgt
     9361 cgaccccgcc gagctcgaag gagccgctcg tcggctgctg cagccgcgcg aggagctgcg
     9421 ccagctcgct cgcgcccgag ccgggccggc cgacgatcgc gacatgctgg tcgagcccgg
     9481 cctcgaacga cacgctttcc accaggcgcg tgccgtgctc gtcgacgagc gaaaggttcg
     9541 cggcgcgcag cttgccgccc ttcggcagct gcggcgactc gagcagcgcc tgcatctcgg
     9601 gcggcgcaac ctcctcgtcc aggaactgct ccatcacctg ctcgtacttc acctcgacgt
     9661 cgaggcgctg ctggtcccag tcgatcagct ccttcaccgg gccgggcagg tccttgtagg
     9721 ccgcgatcac cgcgaccagc gcgccgatgt cgaggcttcc catgatcacg aggtagccgc
     9781 ccacggtgta gaagagaaac ggcgtcacct gcgagaggaa attgttcagg aacttgatca
     9841 ggaatttccg ctggtagaac tcgaagcgga tgtggaagat gcgcccgagg cgcgccgaga
     9901 tctcggcgcg ctcgtagttc gaggtggcgt tggcgtggat ctcggccgcg ccatcgacgg
     9961 tctcggcgat gcgcccggca agggcgcgcg cggtgagctg gcgctcgcgg ccgagcacga
    10021 tcaggcgctt gcgcagccgg gggatgaagt aggcctgcac gccgaccacc gcgagcgcga
    10081 tcagcccgag cgcccagtgc tggtagagga tgaagaacat ggccgtcgcc gcctggccgc
    10141 cgaggaacag cggcgtgatc agcgcctcgc cgatgaagcc gccgagcggc tcgacctcgt
    10201 ccttgatcat ggtggccatc tcggcggcct tcagccggcg aaaccgcgac agcgggaagc
    10261 gcaggatgtg gtcgtacagc acgtagcgca ggcgccgcag catgcgctcg cccatccagc
    10321 ccttcatcgt gttgatgcgg aacttgagcc agccgttgag cacgatcagg ccgaggaaga
    10381 tcatcgacag cgcgaacagg tactccaggc gcccgagctc cagcccgaag aatccggcgc
    10441 tcgagcttcc gtcggtgaac ctgccgccct ggatcgcctc gttgatgatg gtcttgggca
    10501 ggtcgagcga ggcgtagtac accaccatcg tcagcagcac gaacggcacg atgcgcagct
    10561 ggtcgcgctt gctgtagcgg atgatgaatc cgaagaggct gcgttccacc cgggctttcc
    10621 ccagcgcgca tcttacaagc tatagttggc gcatgacctt cagcgtccgc ttctgggggg
    10681 ttcgcggctc gatcgcttgc tccggcccgc gcaccgcgcg ctacggcggc aacacctcct
    10741 cgctcgaagt gcgctgcggc gaacgcatgc tgctcttcga tgccgggacc ggcttgcgtt
    10801 acctgggcaa ctcgctttcc agtgcgcagc ccgcatctca acccgtggac gccgatcttt
    10861 tcctgaccca cacgcatttc gaccacgtct gcggcctgcc cttcttccgc ccgttcttcc
    10921 agccgcagaa ccgatttcgc ctctgggccg ggcacctcgc cgagggcatg acgctacggc
    10981 gcgtgctcgg cgagttcatg atggcgccgc tcttcccggt gccgccgcag gtgttccgcg
    11041 cgcgcatgga ataccgcgag ttcaaggcgg gcgagacgct gcgccccgcc gagggcgtcg
    11101 cggtgcgcac cgcgacgctc aaccatcccg acggagccac cggctatcgc gtcgagtacg
    11161 gcggccgctc ggtctgctac ctcaccgata ccgagcacgt tcccggcgcg cccgaccgcc
    11221 atatcctcga gctcatcgcc ggcgccgatc tcgtgatcta cgacagcatg tacaccgatg
    11281 ccgagtacga cacgtatgtc ggctggggac attcgacctg gcaggaaggg gtgcgcctgt
    11341 gccgcgccgc cggcgcgaag cgcctcgccg tgttccacca cgatccggag cacgacgacg
    11401 acatgctcga cggcgtggcg cgcgaagtcg caagagagtt gccggggtcg atcgtggcgc
    11461 gagacggcct ggttgttgag cttaattgaa cgctacgccg ccgagctcga agccatcggc
    11521 gccgagccag cgccggcgcc gcgctgggac caggactggt tcccgcggct cgacgccgcc
    11581 gcggcgtatg ccatggtgcg cagcacgcgt ccgcggcgca tagtcgaagt cggctcaggg
    11641 cattcgacgc gcttccttgc gcgcgcggtc gccgacggcg ggctcgacac ccgcatcacc
    11701 gcgatcgacc cgaagccgcg cgccgcgatc cgcggcctga acgtcgaatg gctgcaggcg
    11761 cgcgtggaga cgctggatca cgggccgttc gcgcggctcg gtgaaaacga catcctgttc
    11821 atcgactcga gccaccagct caggccgggc agcgacgtcg agttcctcgt gaaccaggtg
    11881 atcccccggc tgccggccgg ggtgcacgtg cacttccacg acatcttcct gcccgacgac
    11941 tatcccgtgc ggtgggcctg gcggcgctac aacgagcagg ccgcagttgc ttctctcatt
    12001 gaaaataacc tgttcagaat cgatttttcg agccacgtca cggtctccag gtcacccgag
    12061 aagatcgcag gcgtgctcgc gcgcctgccg ctcgtccctg gcgcggtcga atcgagcctg
    12121 tggctgacga agctctcgac atccagccgg taatcgactg gctggtcgaa ggcgcgcggc
    12181 ccgcgcggcg gccgcaggac gtgctggtcg atacatgcca gcgcacggtg tccgctgggc
    12241 tacccctcta ccgcgtcggc gtgttcgtgc gcacgctgca ccccaacgtg ctcggccgtg
    12301 cgttcatctg gcaggcggac acgggcgcgg tcgagatcag cgaagccgcc cacgacctgc
    12361 tcgagtccga aacgtttctg aagagcccga tccgcgtggt gttcaccgag catgtcgagg
    12421 tgcgccggcg gctcgccgat ccggcgtgcc caatggactt tcccatcctg gaagacctgc
    12481 gcaaggaggg cgcgaccgac ttcgtcgcgg cgccgttgcg cttcgtgaac ggcgaggtgc
    12541 atgccgcgag cttcgccacg cgccgcgccg ggggttttcg cgacgccgag ctcgccgcgc
    12601 tgcgccggct gctcgccccg ttcacgcgca tggtcgagat ctacggcaac atgcagaagg
    12661 cgcgcaacat cctcgacgcc tacctcggcc ggggtgccgg ggagaaggtg ctcgccgggc
    12721 aaatcaagcg cggcgacggc caggacatcg acgcggtgat ctggttctgc gacctgcgcg
    12781 actccgcggc gctcgccgac tcgatgagcc ggcgcgaatt cctggccctc ctgaacgaat
    12841 atttcgagtg cgtgctcggc ccggtgctcg agcgccaggg cgaggtgctg cgcttcatcg
    12901 gcgatgccgc gctcgccatc ttcccggtga acggccggcc cgccgaggcc tgcgccaagg
    12961 cgctcgcagc cgcccaggag gccttcgcca ggatggataa gctgaacaaa aagaggcact
    13021 caccgctgcg cttcggcatc ggcctgcacc tgggcgagct tacctacggc aacgtgggga
    13081 cgccctcgcg catcgagttc acggtcgtcg gcgcggccgc caacgaggcg gcccgcatcg
    13141 agagcctttg caagcagctg ggggtggacc tcctggtctc ggagcgcgtc gcgcgggcgc
    13201 tgccgggccc ctggaaatcg ctcggcagcc acacgcttcg cggcgtcgga gataagatgg
    13261 agttattcac tttgtcctga cctccggaag agaatgaata gcgtgacggc tctcggcggg
    13321 cttttcgccg actggtactg gaggatggac ggccagctcc gcctcaccta cacctcgcac
    13381 cagttcgccg agaagaccgg cctcgacccc gccgacgact actgggagca caaccgccgc
    13441 cagctcgagc gccaccaggg cttccgcgat ttcgagatcc ggcgcatcgc ctccgacgag
    13501 cgctgcgtat ggctcgcgct cagcgccgag ccggtgttcg acgagagcgg gactttccag
    13561 ggctaccacg gcgtcggccg caacatcacg gcgcacaagc gcggcgagca ggtcctgcgg
    13621 ctcgagcacg cggtggcgcg cgccctcgcc cagtcgcccg gcgtcgccga gggcctgcag
    13681 gccgggctgc gcgcgatgtg cgacatcgag ggctgggact acggccgctg cttccgggtc
    13741 gacccggcga gcgccgagat cactttcgag gagggctggt tcgcgcgcga gcccgccacc
    13801 gagcagttcc tcagccgcgc gcgcgccacc tggcaggaag gcaagccggt gttcgcaaaa
    13861 tcgccgcgcg attcgagcgg gtctttcgcc accttcgcct tccccgcggt cgcccagggc
    13921 aagaccatcg gcctgctggt gttttccggg caccgcagcg gcgaccccga cgagcggctg
    13981 ctcgaggcgg cgcaggcgat cggcagcctc ttcggccagt tcctgcagcg caagcagacc
    14041 gaggagctgt tgcgcgcgag cgaagcgcgc ttccgcagcc tgacgcatct gtcgtcggac
    14101 tttttctggg agaccgacgc ccagcaccgg ctgacgagca tcgtgcacgg cccgagctac
    14161 ggctcgccgc aggtcgcgcg cgggatcatc ggcaagacgc cctggggcgt gccctcggcg
    14221 agccccgacc aggcaggctg ggcggcgcac atggcggtgc tcaacagcca cctgccgttc
    14281 cgcgacttcg agttcgcccg cgtcatgccc gacggcggca cccgctacct ctcggtgggc
    14341 ggcgagccgc gcttcgatgc gatgggcgta ttcctcggct atcgcggcat cgggcgcgac
    14401 gtgaccgaga tctcgctcgc ccgcgagcgc atcgcctcgc tcgcctataa cgacgcgctg
    14461 accggcctcg cgaaccggac cagcctctcg ccggcgctcg agcaggcaat cgagcgcacc
    14521 cgccgccacc gctccaagct cgcgggcgtg ttcatggacc tcgacggctt caagcagatc
    14581 aacgacctgc acggccacgc ggccggcgac gctttcctcg tcgaggtcgc gcgccgcctg
    14641 cgccgcagcc tgcgcgcaag cgacgtggtg gcgcgcctgg gcggcgacga gttcttcgtg
    14701 gtgctggagc aaatgcagga cgcgccggcg gtcgagcgcg tggtgagcaa gctcgcgagc
    14761 gaggtgctga agccctacga gctcttcggc ggcgtcaaga cgcgcatctc ggcgagcctg
    14821 ggcgtgagca tcttccccga cgacgccggc gatgccgcca ccctgatggc gcacgccgac
    14881 aaggcgatgt acgcggccaa gcgcgccggc aagaacgcgt tctgcatctt ctcctcggga
    14941 acctccggca gcccgacctc gatcccctcc tcggaccacc gggcggcgta aagatcccgt
    15001 taatttcctc gccgcgcggt agaactcgcg gcagccatgg cctaggctgg cccgccaatg
    15061 ggccccgagg acaaccccga agatacctat cgcgccgcgc tggttcgcgc tgcccatctc
    15121 ctcggcggcg cccgccgcct gggcgagcgc ttgcaggtgc ccatgccgga gctcacgcgc
    15181 tggctggccg gcgatggcaa gccgagcatc ggcacgttcc tcaaggtcat cgacgtgctt
    15241 atcgaagaga gcgggaaacc ggccgagtaa cgcatccctc gtattgcggg tctgccagca
    15301 gtgtaataca gtacccgcgt gaagactctc acagtgcgcc tgccggaatc gctcgcagcc
    15361 tccatcgagg ccgaatcccg cgcgcgcaag ctctcgaagt ctgacgtcgt gcgcgagcgc
    15421 ctcgagcgcg cgccggctgg cgccgcggag cccaaccctg cttcctacga catcgccgat
    15481 ttgatcggct cggttgacgg cctgcccgct gatctgagcg ctcgaaagaa gcattacttg
    15541 cgcctcgggt atggccgcaa gcgttctcgt tgacgcgagc ttcgtcgtag ccctcttcaa
    15601 ccgtcgagac agcaatcacc gctgggcggc cgcgcagggc tccaatcatg cgcgtccatg
    15661 gcacacctgc gaggcggcgc tctccgaggc cttttacctg ctagggccgc gcggcgctcc
    15721 agcactgagg acgtttctga ggcggggttc tctccggctg gcgttcgacc tcgatggcca
    15781 gctcgacccg gtgctccagc tcatcgagaa gtacgccagc gtgcccatga gcctggcgga
    15841 cgcatgcctg gtacgcatga ccgagacgct ttcgaatccg ctgcttctga ccatcgattc
    15901 cgatttccgg atctatcgcc gtcacagcag gcaggtcgtg ccctgcgtga tgccggacta
    15961 gtcggcgctt aggagatgca tccgagctat tgaatgatct gacgcctcgc acgagcaacg
    16021 gcgcggggat gcgcaggccg agggcgcgag ccgccttgag attgacgacc aggtcgagct
    16081 tggcgacctg ctcgaccggc atctaggcgg ccttcgcgcc cttgaggatc cggtcgacgt
    16141 agcgcgccgc gatgcaatag tcctcccgaa aatccggccc gtaggacagc aacccgccga
    16201 cgtcggtgaa ctggcgatgc tcccaaaccg atgggatccc attgcgcatc gccagatcca
    16261 cgagctgtcg ccggtgggca gcgaaagttc cggaaggcaa gatgatcagc gcttccgacg
    16321 tcttcgccgc agcgaacgtc gcatcgaatt cctcaggcaa ggatgcgacc aggacctgca
    16381 gctcgattcc gagcgaacgc gccgagctcg tgcatggcgt cggcgaatga tttcatcagc
    16441 ccattcggcg ccggctggcc cagcaagacg ccgaccctgg cgattcttcg cgatggttgc
    16501 gcgcgggcga tccgcgccgc accctgcgcc gccagccaac ccagcaccaa cttgcgccgt
    16561 tgcatgcgta cccccgcagg cacgctaagc ccgccgcgcc agccgtcaag aaaacgtggt
    16621 ctgtccctgt ttttggttct aggtgcgggc ggtggtgctg tagggatcgg cggcgtcgcg
    16681 caggccgtca ccgaagaagt tgaaggcgag cacgatgatg aacaccggga cgaccgagag
    16741 catgagccag ggatagagcg cgaccacgtt gatgttctgc gcctcgttca gcagcacgcc
    16801 ccagctcgtc acgggcggtc ttagccccag gcccaggaag gaaagggcag tctcccccag
    16861 gatcatcgcg gggaccgaga gcgtggcgga tgcgatcagg tggctggtaa agctcgggag
    16921 caggtgcttg cggatgacgc gcgccgggct cgcgcccatc agcaccgcgg cggtcgcaaa
    16981 gtcttcttct cttagggcca acaattttga tcggaccgca cgggcgagcc ccggccagtc
    17041 gagcaggccg aggatgatgg tgatgccgaa atagaccagg atcgggctcc agttcaccgg
    17101 cagcgcggcc gacagcgcca tccacagcgg cagctcggga aacgagcgca ccatctcgat
    17161 gaagcgctgg atcacgttgt cgatccagcc gccgtagaac ccggagatgc ccccgagggt
    17221 gatgccgatg aggaagctca ccgcgatgcc gaacaggccc acggtgagcg agatgcgcgt
    17281 gccgtagacg atgcgcgaga acagatcccg ccccagccgg tcggtcccga ggaggtagag
    17341 cgtgccccgt tccgccgggc agacgaagtg gaagcgcccg gggatcaagc tccagaattc
    17401 gtagtcgtcg ccgaggcaaa agaagcgcaa gcggaacacc gcgctgcggt catccgtgta
    17461 ttcgcgcttc agtgtctgca tgttgaggcg catgctgtag ccgtagacga acgggccgac
    17521 gaagctgccg tcgtggaaga
//
LOCUS       DATBSZ010000091         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_696570, whole genome shotgun sequence.
ACCESSION   DATBSZ010000091 DATBSZ010000000
VERSION     DATBSZ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696570"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VIG70_06060"
     CDS             complement(<1..363)
                     /locus_tag="VIG70_06060"
                     /inference="COORDINATES: protein motif:HMM:NF042805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6781 family protein"
                     /protein_id="HEY8250152.1"
                     /translation="MKTGGDVARQMRDITLEALKNRRFDREGIREVVRAVTEGMAAAA
                     PASGGSMRKLMGQAFRGMDEALTKSVEAGESALRQMVATGRSISDNEVKQALAGLKKI
                     EEDFVATVSQVASSASERT"
     gene            complement(395..1195)
                     /locus_tag="VIG70_06065"
     CDS             complement(395..1195)
                     /locus_tag="VIG70_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8250153.1"
                     /translation="MAGNVVEIRDLHFSFGDLEVLRGLSLDIPRGKVVAILGGSGSGK
                     STLLKLIGGQLRPLRGSVTVEGKNVHRLSAEELYKLRLEIGMMFQTSGLFTDLSVYDN
                     IAFPIRENFPLPEELLRRLVLMKLHAVGLRGAHAMMPADLSGGMTRRVALARAIATDP
                     KLMMYDEPFAGLDPISLNQVGELIRSLNDALGLTSIVVTYDVQEALKVADYVYVIGDG
                     AILGKGTPEEIAASEDPYLKQFVNALPDGPVRFHFPGPPLEEDLRIHG"
     gene            1213..>1671
                     /locus_tag="VIG70_06070"
     CDS             1213..>1671
                     /locus_tag="VIG70_06070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEY8250154.1"
                     /translation="MHPYAALTPEVVLDAVAAAGLRPDGRLLALSSYENRVYQVYLDE
                     GPPVVAKFYRPQRWSEAQIEEEHAFAHELANREIPVVAPSHKGTFGGFIFAVYPRRGG
                     RTPELDNPKVLEWIGRFMGRIHAVGAAKKFIHRESLTPKSFGHDPRAYLLA"
BASE COUNT          277 a          572 c          540 g          282 t
ORIGIN      
        1 cgtgcgctcg ctcgccgagc tcgccacctg gctcacggtg gcgacgaaat cctcctcgat
       61 cttcttcaac ccggcgagcg cctgcttcac ctcgttgtcg ctgatgctgc ggccggtggc
      121 caccatctgg cgcagcgccg attcacccgc ctcgaccgat ttcgtgagcg cctcgtccat
      181 gccgcggaac gcctgtccca tcagcttgcg catcgagccg ccgctcgccg gcgcggcggc
      241 cgccatgccc tcggtcacgg cgcgcaccac ttcgcggatg ccttcgcggt cgaagcgccg
      301 gttcttcagc gcctcgaggg tgatgtcgcg catctggcgg gcgacgtcgc cgccggtctt
      361 catcgcttcg gaagcagcct ttctaagaga gtcatcagcc atggatgcga aggtcctctt
      421 ccagtggcgg tccggggaag tggaaacgta ccggcccgtc gggcagcgcg ttgacgaact
      481 gcttgagata aggatcctcc gacgccgcga tctcctcggg agtgcctttg ccgaggatcg
      541 cgccgtcccc gatcacgtag acgtagtcgg caaccttgag cgcctcctgc acgtcgtagg
      601 tgacgacgat cgaggtgagc ccgagcgcgt cgttcaggct gcggatcagc tcgcccacct
      661 ggttgagcga gatcgggtcg agcccggcga agggctcgtc gtacatcatg agcttcgggt
      721 cggtggcgat ggcgcgcgcg agcgcgacgc gccgcgtcat tccgcccgag aggtcggccg
      781 gcatcatcgc gtgcgcgccg cgcaggccca cggcatgcag cttcatcagc accaggcgcc
      841 gcagcagctc ttcgggcaga gggaaatttt cgcggatcgg gaacgcgatg ttgtcgtaca
      901 ccgaaaggtc ggtgaacaag cccgaggtct ggaacatcat gccgatctcg agccgcagct
      961 tgtagagctc ctcggccgag agccggtgga cattcttgcc ctcgaccgtg acgctgccgc
     1021 gcagcgggcg cagctggccg ccgatcagct tgagcagggt cgacttgccc gagccgctgc
     1081 cgccgaggat ggccaccacc ttgccgcggg ggatgtcgag cgacagtccg cgcagcacct
     1141 caagatcgcc gaaggagaag tgcagatcgc gaatctcgac gacattccct gccatgcaaa
     1201 tactatacgc cgatgcatcc atatgctgca ctcacgcccg aagtcgttct cgacgcggtc
     1261 gccgccgccg gcctgcgtcc cgacgggcgg ctgctcgcgc tgtccagcta tgagaaccgc
     1321 gtgtaccagg tgtacctcga cgaaggcccg ccggtcgtcg ccaagttcta ccgcccgcaa
     1381 cgctggagcg aagcgcaaat cgaggaggag cacgcgttcg cgcacgagct ggcgaaccgg
     1441 gagatcccgg tcgtggcgcc gtcgcacaag ggcaccttcg gcggctttat cttcgcggtc
     1501 tatccgcgcc gtggcgggcg cacgccggag ctcgacaacc cgaaggtgct cgaatggatc
     1561 ggccgcttca tggggcggat ccacgcggtc ggcgccgcga aaaaattcat ccaccgggag
     1621 agcctgacgc cgaaaagctt cggacacgat ccgcgcgcct atctcctcgc c
//
LOCUS       DATBSZ010000092         5028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_81346, whole genome shotgun sequence.
ACCESSION   DATBSZ010000092 DATBSZ010000000
VERSION     DATBSZ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81346"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VIG70_06075"
     CDS             complement(<1..188)
                     /locus_tag="VIG70_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEY8250155.1"
                     /translation="MEVAPVLIAAPILLATYRRFPLTSLLYALICVHALVLILGGAYT
                     YARVPLGFWLQDLLELER"
     gene            complement(266..1186)
                     /locus_tag="VIG70_06080"
     CDS             complement(266..1186)
                     /locus_tag="VIG70_06080"
                     /inference="COORDINATES: protein motif:HMM:NF005792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HEY8250156.1"
                     /translation="MSVFAIIAALVVEQWRPLGERKSVSAALAAWAAYLERAFNGGER
                     QHGMVAWLVAVLPPVAAAILLHALLSAAGWVFALAFEIALLYLTLGFRQFSHYFTDIQ
                     LAIKADDIEQARFLLDQWRGASGVVRTREEVVRLAIEEALVAAHRHVFGVLLWYLVLP
                     GPAGAILYRLAAYLAVRWRPLGAFGRFAAEAFHILEWPAVRLTAAAFALVGDFENTVY
                     CWRTQARTWPDPDAGVLLSAGAGAMGVRLGMPVQDVDGPQPRPELGVGEAADGPFLDT
                     TVGMLWRALVLWVAVLAGAALVAHSSRFWA"
     gene            complement(1183..1770)
                     /locus_tag="VIG70_06085"
     CDS             complement(1183..1770)
                     /locus_tag="VIG70_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY8250157.1"
                     /translation="MPLDLAELRRRLASSSAPEFTLFGDDGTEGRDAASTAAAVLIPL
                     IAHGELTVLFTQRTTHLKSHAGQVSFPGGRAEPGDASAEFTALREAGEEIGLAAERVE
                     ILARLPDYRTRTGFRVTPVVGLVTPPLALAPDPREVAEVFEVPLAFLLDPRNRQRRTR
                     EFQGQQVGYYVFEYQERVIWGATAGMLVNLHKMLE"
     gene            1806..2054
                     /locus_tag="VIG70_06090"
     CDS             1806..2054
                     /locus_tag="VIG70_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250158.1"
                     /translation="MTRIALAAALVAAAFIATPLAFANNCPNEWKAIDAALPKAKLDD
                     KQMAEVKKLRAEGEKLHKEGKHSESMAALGKAKKILKI"
     gene            complement(2061..3032)
                     /locus_tag="VIG70_06095"
     CDS             complement(2061..3032)
                     /locus_tag="VIG70_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY8250159.1"
                     /translation="MAKAIRYHKQGGPEVLQIDEVTVGDPGPGQARVRHKAIGVNFVD
                     TYQRSGLYPMQLPGTAGNEAAGVVEAVGAGVSNVRAGDRVTYTGLPGSYCDVRLVPAD
                     RLVKLPDGVTEEMAASMTLKGMTVQYLIHRTYAVKSGDTVLWHAAAGGVGLIAGQWLK
                     AIGATGIGTVGSEEKAALAKSHGWQHVINYTKENFVEKVKSITGGKGLPVVYDAVGKT
                     TWEGSLECLRPRGLMVSFGNASGPVAPVNLGILSQKGSLYVTRPTLATYIASRPELEE
                     TAKSLFDVVKSGKVKIETTKRYKLDDAAQAHRDLEGRKTTGSVVLVP"
     gene            complement(3044..4072)
                     /locus_tag="VIG70_06100"
     CDS             complement(3044..4072)
                     /locus_tag="VIG70_06100"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HEY8250160.1"
                     /translation="MFAYGHAAAPTWRECASACAERLGRPDRGLGFVYFSDALVGSAQ
                     EILDTLRSRTGVEDWVGSVGVGVLATGAEYQDEPAMAAMVADIDAFSVFSGRAPLKGG
                     HFAVVHADPSAPDVAGLIADMSAKVASGFLVGGLSSSRSRTVQIANAVLSGGLSGAAL
                     APEVAVATRLTQGCVPYPGRWRITECTENIIGTLDGRPALDVLKEVVGNDRQVLVGLP
                     IPGSDTGDYTARNLVGIDPRNKLIAIGDRVEPGTELVFCRRDQAAAREDLQRISKELK
                     SRVPQPRGALYFSCLARGEHMFGARGAELELIRRALGEVPLVGFFCNGEISRDRLYGY
                     TGVLTLFH"
     gene            complement(4073..4333)
                     /locus_tag="VIG70_06105"
     CDS             complement(4073..4333)
                     /locus_tag="VIG70_06105"
                     /inference="COORDINATES: protein motif:HMM:NF043076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HEY8250161.1"
                     /translation="MPDDSPPQDPFEMFRRLWGPLGVPIPGMAMPTFDPKEVEKRIAE
                     LRSVEAWLSMNLNMLKMSIQGLELQRAALQAMKSASEPPKKE"
     gene            complement(4326..>5028)
                     /locus_tag="VIG70_06110"
     CDS             complement(4326..>5028)
                     /locus_tag="VIG70_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8250162.1"
                     /translation="TSGQSVSFFQGGSGLEGWERNQRIGAAVPISLDLLLASAALPFI
                     FPAVKFNREYFGDGSMRQIAPVSPALHLGADRVLIVGTGRQTGEEARVRSNVYPSLAQ
                     IAGHALNSIFLDSLMVDIERLERINRTVQLIPQDRLSDSGVQLRPVKVLFITPSQPIE
                     RIAARFIHELPRTVRFILRPTGALNRSGSNLASYLLFEESFCRALIDLGYRDTVAREA
                     EVREFFSLEENRAHA"
BASE COUNT          857 a         1738 c         1700 g          733 t
ORIGIN      
        1 ttgcgctcga gctcgagcag gtcctgcagc cagaagccga gcggcacgcg cgcgtacgtg
       61 taggcgcccc cgaggatgag cacgagggcg tgcacgcaaa tgagcgcata caagaggctg
      121 gtcagcggga agcgccggta agtggcgagg agaatcggcg ccgcgatcag gacgggtgcg
      181 acctccatca gccacgttgc ccggtcatag ggcttgattc ccgaaacgac caacgccccg
      241 agcacaacaa ccaatcccag cagcttcaag cccaaaaccg cgagctatgc gcgacgagtg
      301 ccgccccggc gagcacggcc acccacagca ccagcgcgcg ccacagcatg cctacggtgg
      361 tgtcgaggaa cgggccgtcg gccgcctcgc cgacgccgag ctccggccgc ggctgcgggc
      421 cgtcgacgtc ctgcaccggc atgcccaggc gcacgcccat cgcgccggcg cctgccgaga
      481 gcagcacgcc cgcgtcggga tcgggccagg tgcgcgcctg cgtgcgccag cagtacaccg
      541 tgttctcgaa atcgccgacc agcgcgaacg cggccgcggt aagccgcacc gccggccatt
      601 cgaggatgtg gaaggcctcg gcggcgaagc gcccgaatgc acccagcggg cgccagcgca
      661 cggcgaggta ggcggcgagc cggtagagga tcgccccggc cggcccgggc agcaccaggt
      721 accacagcag cacgccaaac acgtggcgat gcgcggcgac cagcgcctcc tcgatcgcga
      781 ggcgcaccac ttcctcgcgc gtgcgcacca cgcccgaggc gccgcgccac tggtcgagga
      841 ggaagcgcgc ctgctcgatg tcgtcggcct tgatggcgag ctggatgtcg gtgaagtagt
      901 ggctgaactg ccggaagccc agcgtcaaat aaagcagcgc gatctcgaac gcgagcgcga
      961 acacccagcc cgccgcagaa agcagagcgt gcagcaggat cgcggccgcc accggcggca
     1021 gcaccgcgac cagccaggcg accatgccgt gctggcgctc gccgccgttg aacgcgcgct
     1081 ccaggtaagc cgcccaggcg gcgagcgcgg ccgagacgct tttcctctcg ccgagcggac
     1141 gccattgctc gaccaccagc gccgcaatga tcgcaaaaac gctcattcga gcattttatg
     1201 caggttaacg agcatcccgg cggtcgcgcc ccagatgacc cgctcctggt attcgaacac
     1261 gtagtagccg acctgctggc cctggaactc gcgcgtgcgc cgctgccggt tgcgcggatc
     1321 gagcaggaac gcgagcggaa cctcgaacac ctcggccact tcccttgggt cgggtgcgag
     1381 cgcgagcggc ggcgtgacca gcccgacgac cggcgtcacg cgaaagccgg ttcgcgtgcg
     1441 gtagtcggga agccgcgcca ggatctcgac gcgctcggcg gcgaggccga tttcctcgcc
     1501 agcctcgcgc agcgcggtga actccgcgct cgcgtcgccg ggctcggcgc gcccgccggg
     1561 aaagctcacc tggcccgcat ggctcttgag gtgcgtggtg cgctgggtaa acaggacggt
     1621 caattcgccg tgagcaatca aggggatgag caccgcggcg gccgtgctcg ccgcgtcgcg
     1681 gccctcggta ccgtcgtcgc cgaagagcgt gaattccggc gcactggagc tcgcgaggcg
     1741 cctgcgcaac tcggccagat caagcggcat aatgcgggcg taggggccaa cccaggagaa
     1801 aacgcatgac ccgcattgca ctcgccgcag ccctagtcgc ggccgccttc attgccaccc
     1861 cccttgcctt cgccaacaac tgcccgaacg aatggaaggc gatcgacgcc gcgctaccca
     1921 aggccaagct cgacgacaag cagatggccg aggtgaagaa gctccgcgcc gagggcgaga
     1981 agcttcacaa ggagggcaag cactccgagt ccatggccgc gctcggcaaa gcgaagaaga
     2041 tcctgaagat ctagatctcg ctaggggacc aggacgaccg aaccggtcgt cttgcgtccc
     2101 tccaggtcgc ggtgcgcctg cgccgcgtcg tccagcttgt agcgcttggt cgtctcgatc
     2161 ttcaccttgc ccgacttcac cacgtcgaac agggatttcg cggtttcctc gagctcgggg
     2221 cgcgaggcga tgtaggtggc gagcgtgggg cgcgtgacgt agagcgagcc cttctgcgac
     2281 aggatcccga ggttcaccgg cgccaccggg ccggacgcgt tgccgaacga caccatcagg
     2341 ccgcgcggcc gcaggcactc gagcgagcct tcccaggtcg tcttgccgac cgcgtcgtac
     2401 accaccggca ggcccttgcc gccggtgatc gacttcacct tctcgacgaa attttccttg
     2461 gtgtagttga tgacgtgctg ccagccgtgc gatttcgcga gcgccgcctt ctcctccgag
     2521 cccaccgtgc cgatgccggt ggcgccgatc gccttcagcc actggccggc gatcaggccg
     2581 acgccgcctg cggcggcgtg ccaaagcacc gtgtcgcctg atttgaccgc ataggtgcgg
     2641 tggatgaggt actgcacggt catgcctttc agcgtcatcg acgccgccat ctcctcggtg
     2701 acgccgtcgg gcagcttcac caggcgatcg gccggcacga ggcgcacgtc gcagtaggag
     2761 cccggcagcc cggtgtaggt cacgcggtcg ccggctctta cgttgctcac gccggcgccc
     2821 acggcttcga ccacgccggc ggcctcgttg cccgcggtcc cggggagctg catcggatac
     2881 aggcccgagc gctggtaggt gtcgacgaaa ttcaccccga tcgccttgtg gcgcacgcgc
     2941 gcctggcccg ggccgggatc gccgaccgtg acttcgtcga tctgcaaaac ctccggtccg
     3001 ccctgcttgt gataacggat tgccttcgcc atttcgtttc tccttagtgg aatagggtca
     3061 gcacgccggt atagccgtac aaccggtcgc gcgaaatctc gccgttgcag aagaatccta
     3121 ccagcggcac ctcgccgagc gcgcgccgga tcagctcgag ctcggcgccg cgggcgccga
     3181 acatgtgctc gccgcgcgcg aggcaggaaa agtacagcgc gccacggggc tgcggcaccc
     3241 tcgatttcaa ttccttcgaa attctctgta gatcctcgcg cgcggcggcc tgatcgcgcc
     3301 ggcagaacac cagctcggtc cccggctcga cccgatcgcc gatcgcaatc agcttgttgc
     3361 gcgggtcgat gccgacgagg ttgcgcgcgg tgtagtcgcc ggtgtccgag ccgggaatgg
     3421 gcaggccgac caggacttgc ctgtcgtttc cgaccacttc cttcagcacg tcgagggcgg
     3481 gccggccgtc gagggtcccg atgatgtttt ccgtgcattc ggtgatacgc cagcggcctg
     3541 ggtacggcac gcagccctgc gtgaggcgcg tcgccaccgc cacctcgggc gcgagggcgg
     3601 cgccggaaag cccgcccgaa agcacggcgt tcgcgatctg caccgtgcgc gagcgcgagc
     3661 tcgacagccc gccgacgaga aagccgctcg ccaccttggc cgacatgtcg gcgatcagcc
     3721 cggcgacgtc gggcgccgag ggatcggcgt gaaccaccgc gaaatgcccg cccttgagcg
     3781 gcgcgcggcc ggagaagacg ctgaaagcat cgatgtcggc gaccatcgcc gccatcgccg
     3841 gctcgtcctg gtattcggcg ccggtggcga gcacgccgac gccgacgctt ccgacccagt
     3901 cctcgacgcc ggtcctgctg cgcagggtgt ccaggatttc ctgtgccgag cccaccagcg
     3961 catcgctgaa gtagacgaag cccaagccgc ggtcggggcg cccgaggcgc tcggcgcaag
     4021 ccgaagcaca ttcgcgccag gtgggcgccg ccgcgtggcc gtaagcaaac atctattcct
     4081 tcttcggcgg ctccgaggcg gacttcatgg cttgaagcgc cgcgcgctgc agctcgaggc
     4141 cctggatcga catcttgagc atgttgagat tcatgctgag ccacgcctcg accgagcgca
     4201 gctcggcgat gcgcttctcg acttccttgg ggtcgaaggt gggcatcgcc atgccgggga
     4261 tcggcacgcc gagcggcccc cacagccggc gaaacatctc gaacggatcc tgcggcgggg
     4321 agtcgtcagg catgggcgcg gttttcttcc aaactaaaga attcgcggac ctcggcctcg
     4381 cgtgcgaccg tgtcgcggta gccgaggtcg atcagcgcgc ggcagaacga ctcctcgaag
     4441 agcaggtagc tcgcaagatt ggagcccgag cggttgagcg cgccggtcgg gcgcaggatg
     4501 aagcgcaccg tgcgcggcag ctcgtggatg aagcgcgccg cgatgcgctc gatcggctgc
     4561 gacggggtga tgaacagcac cttcaccggg cgcagctgca cgccggagtc ggacagcctg
     4621 tcctgcggaa tcagctgcac ggtgcggttg atgcgctcga ggcgctcgat gtcgaccatc
     4681 aggctgtcga ggaagatgct gttcagcgcg tggccggcga tctgcgccag cgacggatag
     4741 acgttggagc gcacgcgcgc ctcctcgccc gtctgccggc cggtgccgac gatgagcacg
     4801 cggtcggcgc caaggtgcag cgccgggctg accggcgcga tctggcgcat cgagccgtcg
     4861 ccgaagtact cgcgattgaa cttcaccgcc gggaagatga agggcagcgc cgccgaggcg
     4921 agcagcaggt ccaggctgat cggcaccgcg gcgccgatgc gctggttgcg ctcccagccc
     4981 tcgaggccgc tgccgccctg gaaaaacgag acgctctgcc cggaggtg
//
LOCUS       DATBSZ010000093         6626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2404590, whole genome shotgun sequence.
ACCESSION   DATBSZ010000093 DATBSZ010000000
VERSION     DATBSZ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2404590"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VIG70_06115"
     CDS             complement(<1..1106)
                     /locus_tag="VIG70_06115"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004815.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8250163.1"
                     /translation="MSSDLCTLSATELVDAFRKKTLSPVDVASAVLKRIEKLNPVFNA
                     FNLVSDRALEDAKASEARWLAGQPKGLLDGVPVSIKDIVLTKGWPTLRGSKTIDPKGP
                     WNDDAPATARLREHGAVLLGKTTTPEFGWKGVTDSPLTGITRNPWNKEKTPGGSSGGA
                     AAAVAAGMGPLAVGTDGGGSIRIPCSFTGLFGIKASFGRVPAWPLSPFGTVAHVGPMT
                     RSVADAALMLNVLAQPDARDWHALPYDPRDWRTGLEQGIADLRIAYSPDLGYAKVDPE
                     IRKIVQDAVSVFSDLGAKVEEKNPGFADPAPIFMTHWFSGAAFLLKGIPKEKHKLVDP
                     GLLEMAAQGEKVTAHDLLDAQLKRGALGTHMNVF"
     gene            1140..2474
                     /locus_tag="VIG70_06120"
     CDS             1140..2474
                     /locus_tag="VIG70_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8250164.1"
                     /translation="MGPQTRYALSGDVNIAYQVIGEGPVDLVFVMGWVSNIDEFWTEP
                     SFAHFLQRLARFSRLIVFDKRGTGLSDRVDEKNVPTLEQRMDDVRAVMDAVGSRKAAL
                     LGISEGGPMCALFAATYPERTAALMTFGSFARPLAAPDYPWGRTEEAQRQFVEAIRTG
                     WGSKPVGIEARVPSKAHDEAFRQWWTRYLVRSASPSAVLALVRMNILIDVRAVLPAIR
                     VPTLVMHRTGDLALDVGGSRYMAQQIPGARFAEYPGVDHLPWTSDPEPVLGDIEQFVT
                     GARHAPEPDRVLATVMFADIVDSTQRAAAIGDRAWTRELQEFYGLARRQLERFRGREI
                     DTAGDGYFATFDGPARAVRCAQNLSREMSQLGLTIRAGLHTGEVEVIGEKVGGIAVHI
                     GARIAGHAGPGEVVVSNMVKDLVAGSGLQFEDRGVRPLKGVPGEWRLYRASP"
     gene            complement(2471..3184)
                     /locus_tag="VIG70_06125"
     CDS             complement(2471..3184)
                     /locus_tag="VIG70_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY8250165.1"
                     /translation="MDQTLLDDLVAANRILAEHGVIDAYGHVSIRSPHDPKRFFLARN
                     IAPETVQAGDLLEYGLDGEPVDAGRARDSVSERYIHSEVYRARPEVMAVVHNHSPSVV
                     PFSVTNVPMRALFHMAAFVGEGVPNFEIRKVKKGSDLLIRTPALGKALAKTLAAKPAC
                     LMRGHGSVVVGENLPRAVGRSVYLEISARMQMQALLLSKKITYFDAAEVKASNPVQDY
                     KRAWPLWREKALAKAKAER"
     gene            complement(3184..4137)
                     /locus_tag="VIG70_06130"
     CDS             complement(3184..4137)
                     /locus_tag="VIG70_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250166.1"
                     /translation="MRGIAAAVLASCLAGGALAQEFPSRTVHLIVPYTPGTGADTLAR
                     LLGARLAETWKVGVVTENKTGATGNIGAAHVAKSAPDGYTLLFVATSFGTTPAVSRAP
                     FDPVTDFAPVALVATSGLVIVANPRLAARTMREFIELAKREPGKLHYSSPGNGGPQHL
                     AMELLKLELGLDIVHVPYRSAAPAISDVVGGHVHATIAAVQTAQPQIASGQLRALAVM
                     SAERSQAFAQVPTMKEQGMPELEVETWYGAFAPAGTPVAVVSRVNADLNSLLKEAAIR
                     EMLAKQGMTAKGGPPERLGELLMRELARWKRVVDAAGIKAD"
     gene            4136..5737
                     /locus_tag="VIG70_06135"
     CDS             4136..5737
                     /locus_tag="VIG70_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY8250167.1"
                     /translation="MRLIIISRLLDTEVLIVGAGPVGLVLAIDLGQKGVRCTLIERKP
                     APQFLPKMERCNARTMEIFRRMGLAARVRAAGLRADVPMDVYIITAMNQPPLLRHAYP
                     SVAEARRQIDACTDASMPLEPYQLISQYTLEPLLKSVAESLPSVSVRYGCEFLSFSQD
                     SVSVTTQTSAGPIRSQYLVGCDGGASAVRQQLDIALHGEGDLLRLWQGLFHCEELFDR
                     IPIANGPGRGRHYHVADERSTFLIMQDSTRHWTLHAQLDDERAMAPQFEKAIGVPVKY
                     EMLYAGEWKQNLLIADRYGAGRVFLAGDAVHLVIPTGGLGMNSGVGDAVDLGWKLAAT
                     LQGWGGPKLLASYEAERRPVGARNVAASRYASQGRRKWRGLWRNLDELVAVAEVEQRK
                     SNEMIGAELGYRYAGSPIIADEPGGPDDDFRQYVPTTWPGARLPHVWLDVHTAIQDRI
                     GRGYTLLRLGNSRPESAGLEKAMKALGAPFEVLEVPDQVARDLYGVDLILLRPDLHVA
                     WRGNRLPAEPSQLAALVTGHYPLGR"
     gene            complement(5719..6045)
                     /locus_tag="VIG70_06140"
     CDS             complement(5719..6045)
                     /locus_tag="VIG70_06140"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY8250168.1"
                     /translation="MTVYVIADIKVTDDRWVPPYAASVHDLVHEHGGKYLSRSGNVKT
                     LEGKPLDTTLIAIIQFPSAEAVEAFVTDPRYARYGAARRDGSESRFQLIDDTDLAGTI
                     PYLPRG"
     gene            6295..>6626
                     /locus_tag="VIG70_06145"
     CDS             6295..>6626
                     /locus_tag="VIG70_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8250169.1"
                     /translation="MVLLRELVAGSTRFNDLRRGVPKMSPTLLSQRLKELEAAGVIER
                     RRLKSEKGVFEYQMTASGRDLRPVVEAVGFWGQKWVEAQLSLKNLDPSLLMWDMRRNL
                     NPSPLPAGR"
BASE COUNT         1033 a         2201 c         2235 g         1157 t
ORIGIN      
        1 tggaagacgt tcatgtgcgt cccgagcgcg ccgcgcttga gctgggcgtc gagcaggtcg
       61 tgcgccgtca ccttctcgcc ctgcgccgcc atttcgagca gcccggggtc caccagcttg
      121 tgcttctcct tcgggattcc cttgagcagg aacgccgcgc cggagaacca gtgcgtcatg
      181 aagatcggag cggggtccgc gaacccgggg ttcttctcct cgactttcgc tccaagatcg
      241 ctgaataccg agaccgcgtc ctgaacgatc ttcctgattt cgggatccac tttcgcgtat
      301 ccgagatccg gcgaatacgc gatgcgcagg tcggcgatcc cttgctcgag gccggtgcgc
      361 cagtcgcgcg ggtcgtaggg cagggcgtgc cagtcgcgcg cgtcgggctg cgcaagcacg
      421 ttcagcatga gcgcggcgtc ggcgacgctg cgcgtcatcg gcccgacgtg cgccacggtg
      481 ccgaagggcg agaggggcca cgccggcacg cggccgaacg aagccttgat gccgaacagt
      541 cccgtgaagc tgcacgggat gcggatcgag ccgccgccat cggtgcccac cgccagcggt
      601 cccattccgg cggcgaccgc cgctgcggcc ccgccggacg atccgcccgg ggtcttctcc
      661 ttgttccagg gattgcgcgt gatgccggtg agcgggctgt cggtcacgcc cttccagccg
      721 aattcggggg tcgtggtttt tccgagaagc accgcgccgt gctcgcgcag gcgcgccgtc
      781 gccggcgcat cgtcgttcca cggccccttc gggtcgatcg tcttcgagcc gcgcaaggtc
      841 ggccagccct tcgtgaggac gatgtccttg atggagaccg gcacgccgtc gagcaggcct
      901 ttcggctgtc cggcgagcca acgcgcttcg gaagcctttg catcctccaa agcccggtcg
      961 gaaaccaggt tgaatgcgtt gaatacggga ttcagctttt cgatgcgctt gagcaccgca
     1021 gaggccacgt ccaccggcga caaggtcttc ttgcggaacg cgtcgaccag ctcggtcgcg
     1081 ctgagcgtgc acagatcgga actcatcgcg gcgattgtat ttgataagct ggcggcgcca
     1141 tggggcccca gacccgatat gcgctgagcg gcgacgtcaa tatcgcctac caggtgatcg
     1201 gcgaagggcc ggtcgacctg gtgttcgtga tgggctgggt gtcgaacatc gacgagttct
     1261 ggaccgagcc ttcgttcgcg catttcctgc agcggctcgc ccgcttctcc cggctgatcg
     1321 tgttcgacaa gcgcggcacc gggctatccg accgcgtcga cgagaagaac gtgccgacgc
     1381 tcgagcagcg catggatgac gtgcgcgcgg tgatggacgc ggtgggctcg cgcaaggcgg
     1441 cgctgctcgg gatttcggag ggcgggccga tgtgcgccct ttttgccgcc acctacccgg
     1501 agcgtaccgc cgcgctgatg acgttcggct ctttcgcgcg gccgcttgcc gcgcccgatt
     1561 acccctgggg ccgcacggag gaagcgcaac gccagttcgt cgaagcgatc cgcacgggat
     1621 ggggctcgaa accggtgggc atagaggcgc gcgttccgtc caaggcgcac gatgaggcct
     1681 ttcgccagtg gtggacgcgc tacctcgtgc gcagcgcgag cccgagcgcg gtgctggccc
     1741 tggtgcgaat gaacatcctg atcgacgtgc gcgccgtgtt gccggcgatt cgcgtgccga
     1801 cgctcgtcat gcatcgcacc ggcgacctgg cgctggatgt ggggggcagc cgctacatgg
     1861 cgcagcagat cccgggcgcc cggttcgccg agtacccggg ggtggaccac ctgccctgga
     1921 ccagcgaccc ggagcccgtg ctgggcgata tcgagcagtt cgtcaccggc gcgcgccacg
     1981 cgcccgagcc cgaccgcgtg ctcgcgaccg tgatgtttgc ggacatcgtc gattcgacgc
     2041 agcgcgcggc ggcgatcggc gaccgcgcgt ggaccaggga gctgcaggag ttctatggcc
     2101 tggcgcggcg ccagctcgag cgcttccgcg gccgcgagat cgacactgcc ggcgacggct
     2161 acttcgcgac cttcgatggg ccagcccgcg cggtgcgttg cgcgcaaaac ctgtcccggg
     2221 agatgtccca gctcggcctg acgatccgcg ccggcctgca caccggcgaa gtcgaggtga
     2281 tcggcgagaa ggtcggcggc atcgcggtgc acatcggcgc gcgcatcgcg ggccacgccg
     2341 gcccgggcga ggtcgtggtg tccaacatgg tcaaggatct cgtcgccggc tccggcctgc
     2401 agttcgagga ccgcggcgtg cgcccgttaa agggcgtgcc cggggagtgg cggctctacc
     2461 gcgcctcgcc ctagcgctcg gctttcgctt tcgccagcgc cttctcgcgc cacagcggcc
     2521 aggcgcgctt gtagtcctgc accgggttcg aggccttgac ttcggccgcg tcgaagtagg
     2581 tgatcttttt cgagagcaga agcgcctgca tctgcatccg ggcgctgatc tcgaggtaga
     2641 cgctccggcc cacggcgcgc ggcaggttct cgcccaccac caccgagccg tggccgcgca
     2701 tcaggcacgc gggcttcgcg gcgagggttt tcgccagcgc cttgcccagc gcgggagtgc
     2761 ggatcaggag gtcgcttccc ttcttcacct tgcggatctc gaagttcggc acgccttcgc
     2821 cgacgaacgc ggccatgtgg aacagcgccc gcatcggcac gttggtgacg ctgaacggca
     2881 ccacggaggg cgagtggttg tgcaccaccg ccattacctc ggggcgcgca cggtacacct
     2941 cgctgtggat gtagcgctcg ctgaccgagt cgcgcgcgcg gccggcgtct accggctctc
     3001 cgtcgaggcc gtattccagg aggtcgccgg cctggacggt ttcgggggcg atgttgcggg
     3061 cgaggaagaa gcgcttcgga tcgtgcggcg agcggatgct gacgtgcccg tacgcatcga
     3121 tcacgccgtg ctccgccagg atgcggttcg cggcaaccag gtcgtcgagc agggtttgat
     3181 ccatcagtcg gcctttattc ccgcggcatc cacgacccgc ttccagcgcg cgagctcgcg
     3241 catcaggagc tcgccgagac gctccggcgg gccgcccttc gccgtcatgc cctgcttggc
     3301 gagcatttcg cggatggcgg cctctttcag caacgaattg aggtcggcgt tgaccctcga
     3361 aaccacggcc accggtgtcc ccgcgggcgc aaaagccccg taccaggtct cgacctccag
     3421 ttcgggcatg ccttgctcct tcatcgtcgg aacctgcgcg aacgcctgcg agcgctccgc
     3481 gctcatgacg gcgagcgcgc gcagctggcc cgaagcgatc tgcggctggg ccgtctgcac
     3541 cgccgcgatc gtcgcgtgca cgtgcccgcc cacgacatcg ctgatcgccg gggccgcgct
     3601 cctatagggc acatgcacaa tatcgaggcc gagctcaagc ttgaggagct ccatggcgag
     3661 gtgctgcggg ccgccgttgc ccggcgacga atagtgcagc ttgccgggct cgcgcttcgc
     3721 gagctcgatg aactcgcgca tcgtgcgcgc cgcaagccgc gggttggcga cgatcaccag
     3781 gccgctcgtc gcgaccagcg ccaccggcgc gaagtccgtt accggatcga acggcgcgcg
     3841 cgacacggcc ggcgtggtac cgaaggaagt ggcgacgaaa aggagcgtgt agccgtcggg
     3901 tgcgctctta gccacgtgcg ccgcgccgat attgccggtc gcgccggtct tgttctcggt
     3961 gaccacgccg accttccagg tctcggcgag ccgcgcgccg agcaggcggg cgagggtgtc
     4021 ggcgcccgtt ccgggcgtgt acggcacgat gaggtggacc gttctggagg gaaactcctg
     4081 ggcaagcgct cctccagcca agcaagacgc cagtaccgcc gctgcgatcc ccctcatgcg
     4141 gctcattata atcagccgct tgctcgacac cgaggtcctc atcgtcggcg cggggccggt
     4201 cggattggtt ctcgcgatcg acctcggcca gaaaggcgtg cgctgcacgc tgatcgagcg
     4261 caagcccgcg ccgcagttcc ttccgaagat ggagcgctgc aacgcgcgca cgatggaaat
     4321 cttccgccgc atgggtctcg cggcaagagt acgcgcggcc ggcctgcgcg cggacgtgcc
     4381 gatggacgtc tacatcatca cggcgatgaa ccagccgccg ctgcttcgcc acgcctatcc
     4441 gtcggtcgcc gaggcgcgcc ggcagatcga cgcttgcacc gacgcgtcga tgccgctcga
     4501 gccctaccag ctcatctcgc aatacacgct cgagccgctg ctgaaatcgg ttgccgagtc
     4561 gctgccctcg gtcagcgtgc ggtacggctg cgagtttctg tctttttcgc aggattcagt
     4621 ttccgtcacg acgcaaacca gcgcgggccc catccgttcg caatacctgg tcggttgcga
     4681 cggcggcgcg agcgcggtgc ggcagcagct cgacattgcg ctgcacggcg aaggcgatct
     4741 gctgcgtctc tggcagggac tctttcattg cgaggagctg ttcgaccgga taccgatcgc
     4801 caacggtccg ggaaggggcc ggcactatca cgtcgccgac gagcgctcga ccttcctcat
     4861 catgcaggac tcgacccggc actggacgct gcacgcccag ctcgacgacg agcgcgccat
     4921 ggcgccgcaa ttcgagaagg cgatcggcgt gccggtgaag tacgagatgc tttacgcggg
     4981 cgagtggaag cagaacctgc tgatcgcgga ccgctatggc gccgggcgcg tgtttctcgc
     5041 cggcgatgca gtgcaccttg tgatcccgac gggcgggctc ggcatgaaca gcggcgtcgg
     5101 cgacgccgtc gacctcggct ggaagctcgc cgccacgctc caaggctggg gcgggccgaa
     5161 gctcctggcc tcctacgaag ccgagcgccg tccggtcgga gcgcgcaacg tcgccgcgtc
     5221 gcgctacgcc tcgcaaggcc ggcgcaaatg gcgcggcctg tggcgcaacc tggatgagct
     5281 cgttgcggta gcggaggtcg agcagcgcaa gagcaacgag atgatcggcg ccgagctcgg
     5341 ctaccgctac gccggctcgc cgatcatcgc agacgagccc ggcgggcccg acgacgattt
     5401 tcgccagtac gtcccaacca cctggcctgg tgctcggctg ccccacgtat ggctagacgt
     5461 tcacacggcg atccaggacc ggatcggtcg cggctacaca ctgttgcggc ttggcaattc
     5521 cagacccgag agcgccggac tggaaaaggc aatgaaagcg ctcggggccc cattcgaggt
     5581 cctggaggtt ccggatcagg tcgcgcgtga cctctatggc gtcgacctca tactcctgcg
     5641 gcctgacctg cacgtcgcgt ggcgcggcaa tcgacttccg gccgagcctt cgcagcttgc
     5701 ggcgctcgtc accggccatt acccccttgg caggtaaggg atggtcccgg ccagatcggt
     5761 atcgtcgatc agctggaagc gactttcgct gccgtcccga cgggccgccc cgtagcgggc
     5821 atatctggga tcggtgacga aagcctcgac cgcctcagcc gatggaaact ggatgatcgc
     5881 gatcagggtg gtgtcgagcg gcttgccctc caatgtcttc acgtttccgc ttcgcgacag
     5941 gtacttgcca ccgtgttcat ggaccaggtc gtgtaccgaa gccgcgtaag gcggcaccca
     6001 tctgtcgtca gttaccttga tgtctgctat cacgtaaaca gtcattgtgt ctctcctgtg
     6061 gtcggttgtt cggcaacggg ttatccgtgc gctgaccata gcctcgcagg cgagcgcccg
     6121 agcggtctag tccagaaact gtagcgagcc ggtaacgcgt cggtactagt gccgttacac
     6181 atggctgcca caatcggcac ctcttgccgg aaatggcagc gaatgacgca acccgcatac
     6241 aagcagttct gccctgtcgc catggccgca gagctacttt gcacgcgctg gactatggtg
     6301 ctgcttcgtg aattggttgc cggatcgacg cgcttcaacg atttgcgccg gggcgttcca
     6361 aagatgtccc cgacgcttct gtctcaaagg ctcaaggagc ttgaggcggc cggtgtcatc
     6421 gagcgcaggc ggctcaagtc ggaaaagggc gtgttcgagt accagatgac ggcgtccggc
     6481 agggacctgc gtccggtagt cgaagcggtg ggtttttggg gtcagaaatg ggtcgaggcg
     6541 cagctgtctc tcaagaatct cgatccatcg ttgctcatgt gggacatgcg gcggaatctg
     6601 aatccgtctc cgctacccgc tggccg
//
LOCUS       DATBSZ010000094         7743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2404710, whole genome shotgun sequence.
ACCESSION   DATBSZ010000094 DATBSZ010000000
VERSION     DATBSZ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2404710"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VIG70_06150"
     CDS             complement(<1..273)
                     /locus_tag="VIG70_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel"
                     /protein_id="HEY8250170.1"
                     /translation="MGAHEARERIDAARATLALTVRQRCFYLFCALVLLIVAVPLYEE
                     SRAGRIALNIMSLLILLSGASAIGRGGGSFVISLLLAAPAGIFQMLG"
     gene            complement(292..1605)
                     /locus_tag="VIG70_06155"
     CDS             complement(292..1605)
                     /locus_tag="VIG70_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY8250171.1"
                     /translation="MTPLLAGLILFALLLVLLVGGVWIAIALAAVAWFGLQFFTSTPP
                     DVNLFQSFWGSSASWTLAALPLFVWMGEILYRTKLSEEMFEGLAPWLGWLPGKLMHVN
                     VLGCAIFGTVSGSSTATCATIAKVALPELKRRGYDEKTCLGSLAAAGTLGILLPPSII
                     MVVYAVAAEVSIIKVFLAGFIPGIVLTVLFSGYIVLWALANPGKTPRDETTFSLREKL
                     YRARHLIPCVVLILFIVWVMVIGWATATEAAAYGVLGALAIAWWSGGLSRASFAASLM
                     GATRLSCMIMFILAGASFLSSCMAFTGIPRALAEWVAALAPNKYLLIAILAGIYLLLG
                     TALDGVSMIVLTTSIVLPMVEKAGFDLVWFGIFIVLLVEIAELTPPLGFNIFVLQTMT
                     GRDSNYVALASLPFFFMMVLCIALITVFPELATWLPDWLIGVAKK"
     gene            complement(1602..2135)
                     /locus_tag="VIG70_06160"
     CDS             complement(1602..2135)
                     /locus_tag="VIG70_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY8250172.1"
                     /translation="MRRFLDRLYAASGVLAAICLAAICVVMLAQAFGRTAGVLIRGAD
                     DIVAWLCAASAFLALGHTFRHGELVRVGLWLHKLEPRARRIAEIVALGITALFVGYML
                     WAASRFVYESWAFKEVAQGLIRVPIWIPQLSFVIGVVIFFVAVLDELILVLQKKKPSY
                     QVAEEARIAAGDFSETV"
     gene            complement(2140..3102)
                     /locus_tag="VIG70_06165"
     CDS             complement(2140..3102)
                     /locus_tag="VIG70_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8250173.1"
                     /translation="MLRSLLFAIAALSLGAQAQTKWDMPTPYSEGEFHTRNVKQFVES
                     VQKAAGGKLEIVVHSNGSLIKHPDILRAVSTGQVNIAEFLLSQFGNEDPVFEADNIPF
                     VAAGYDDAWKFYQAQKPLLEKKLQGRGLTLLYSVAWPGQGIYTKEPLKSVADLKGTKF
                     RTYSPLTARLAELLGASPTVVQVPDVPQMFATGGIQAMITSSATGTSTKAWEFVKNYY
                     DTSAFHPKNAVVVNTKGFSRLPADVQQAIMRAASTAETRGWGMSKVRTLEANELLAKN
                     GMTIHKPDAAMQDAYAKVGHQILDEWLKKSGAEGQSIIKAYRGK"
     gene            complement(3132..4265)
                     /locus_tag="VIG70_06170"
                     /pseudo
     CDS             complement(3132..4265)
                     /locus_tag="VIG70_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(4210..5394)
                     /locus_tag="VIG70_06175"
     CDS             complement(4210..5394)
                     /locus_tag="VIG70_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEY8250174.1"
                     /translation="MNKQETSLRLTVLLGVLMALPALGTDLFLPALPVLAQALAADVA
                     AAQLTLTTYFLGLAMGQLLWGPLSDRFGRKPVLLAGLFLLLASSLAAAAVDSVGAVTA
                     ARLVQGLATSSGAVIVRSIVRDLYAHEQAARLLASITIVFSIVPIAAPLGGALLVGSG
                     GWQAVFWCLALAAALLIVATGAGLRETAPGERRSAHPADIARTFRTILAERRFVAPFL
                     LILCAQIGILAWVSNSAFTLVSGLGVSVGAYSVMFAAVMLGQISGAWCSSRLVLRLGI
                     VTLLRSGAALMLAGGLAAAALAWGGVAHWAAVVAPFFAFLLGSALTVPNATASALSPF
                     PKAAGSASSLIGAIAFTAGALISAGLGAAFDGSARPMATVAALAGVAAFLFERFLVRG
                     KR"
     gene            5414..6520
                     /locus_tag="VIG70_06180"
     CDS             5414..6520
                     /locus_tag="VIG70_06180"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY8250175.1"
                     /translation="MQATLANWRTAPFNRWAFHHVRELVPSADIPHDPARARRLPQHA
                     ADLPSDFEVFLRETDTDGIAILHRGKLIFERYANGMTEETPHILMSVSKSLLALLFHE
                     LGLDSALRVPQVVPELESTAYRDATLRDLLDMRAGLVWDENYLATSGPIVEYRKATGW
                     NPTASGETASDLKSFYRTLRETGPHGGRFHYVSPNSDLLGWVIERATGARYAELMSER
                     VWKKVGAERSAYITVDRLGAPRCAGGMCATLRDLARVGQWLIESRRGFLEDLERGDAK
                     AWAAGDLAHYYPGLPMCYRNQWYVLDANRGKSAQDVNAAPLVFGMGIHGQNLFVDRAN
                     EIVIAKFSSQEAPLDPVRISLTMRMVALLRKRFA"
     gene            6513..>7743
                     /locus_tag="VIG70_06185"
     CDS             6513..>7743
                     /locus_tag="VIG70_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY8250176.1"
                     /translation="MPERWQFWIDRGGTFTDVVARRPDGALATHKLLSENPGRYDDAA
                     LAGIREILGVSSDRPLPVHLIEAVKMGTTVATNALLERKGERTALFITRGFRDALRIA
                     YQNRPRIFDRHIVLPEMLYSKVVEVEERVAARGEVVVPLNEDDARTRLAETYNEGYRS
                     IAIVFMHGYRFPEHERRTAELAKAAGFTQISVSHRVSPLMKLVGRGDTTVVDAYLSPI
                     LRRYVEQVAAELRGVRLLFMQSNGGLADARRFQGKDAILSGPAGGIVGAVRTSAAAGF
                     DRIIGFDMGGTSTDVSHYAGELEREFETRVAGVRMRAPMMSIHTVAAGGGSILHFDGA
                     RYRVGPDSAGANPGPASYRRGGPLTVTDANVLLGKIQPAHFPAVFGSSGRESLDREVA
                     TGKFLELQKSIGDGRSVE"
BASE COUNT         1346 a         2590 c         2677 g         1130 t
ORIGIN      
        1 gccgagcatc tggaagatgc cggcaggcgc ggcaagcagc agcgaaatga cgaacgagcc
       61 gccgccgcgg ccgatggcgg aggcgccgga cagcaggatc agcaagctca tgatgttgag
      121 ggcgatacgg cccgcccgtg attcttcgta cagtggtacc gcgacgatca gcagcaccag
      181 ggcgcagaag aggtagaagc agcgctgccg cacggtgagg gcaagggtgg cgcgcgccgc
      241 gtcgatccgt tccctcgcct cgtgcgctcc catgccgcgg attctacgcc gctacttctt
      301 cgctaccccg atcagccaat cggggagcca ggtggcgagc tcggggaaga cggtgatgag
      361 cgcgatgcag agcaccatca tgaagaagaa gggcagcgag gcaagcgcga catagttgct
      421 gtcgcgcccg gtcatggtct gcagcacgaa gatgttgaag ccgagcggcg gggtgagctc
      481 ggcgatctcc accagcagca cgatgaagat gccgaaccac accaggtcga agccggcttt
      541 ctcgaccatc ggcagcacga tcgaggtcgt gagcacgatc atcgacacgc cgtcgagcgc
      601 ggtgccgagc aggaggtaga tgccggcgag gatcgcgatc agcaggtatt tgttcggcgc
      661 gagcgccgcc acccactcgg cgagcgcgcg cgggatgccg gtgaatgcca tgcacgaaga
      721 gaggaagctg gcgccggcga ggatgaacat gatcatgcac gagagccgcg tcgcgcccat
      781 caggctggcg gcaaagctgg cgcgcgacag gccgcccgac caccaagcga tcgcgagcgc
      841 gccgagcacg ccgtaggccg cggcctcggt ggcggtggcc cagccgatca ccatcaccca
      901 gacgatgaac aggatcagca ccacgcaggg gatgagatgc ctcgcccggt agagcttttc
      961 cctcagggaa aacgtggtct catcgcgcgg cgtcttgccg ggattggcca gcgcccacag
     1021 cacgatgtag cccgagaaca gcaccgtcag cacgatcccc gggatgaagc cggcgaggaa
     1081 caccttgatg atcgacacct cggcggcgac cgcgtagacc accatgatga tcgacggcgg
     1141 cagcaggatg ccgagcgtcc ccgcggcggc gagcgatccg aggcaggtct tctcgtcgta
     1201 gccgcggcgc ttgagctcgg gcagcgccac cttggcgatc gtggcgcagg tcgcggtcga
     1261 ggagcccgac acggtgccga agatcgcgca gccgagcacg ttgacgtgca tcagcttccc
     1321 cggcagccag cccagccacg gcgcgagccc ctcgaacatc tcctcggaaa gcttggtccg
     1381 gtacaggatt tcgcccatcc agacgaacag cggcagcgcg gcgagggtcc acgacgcgct
     1441 cgagccccag aacgactgga acaggttgac gtcgggcggc gtgctggtga agaactgcag
     1501 cccgaaccag gcgaccgcgg cgagcgcgat cgcgatccac accccgccga ccagcagcac
     1561 cagcagcagg gcgaacagga tcagccccgc caacagcggt gtcatacggt ctccgaaaag
     1621 tcgccggccg cgatgcgcgc ttcctcggcg acctgatagg agggcttctt cttctgcagc
     1681 accaggatca gttcatcgag cacggcgacg aagaaaatca ctaccccgat cacgaagctg
     1741 agctgcggga tccagatcgg cacgcggatc aggccctgcg cgacctcctt gaacgcccag
     1801 ctctcgtaga cgaagcggct cgccgcccac agcatgtaac cgacgaagag cgccgtgatg
     1861 ccgagggcga cgatctcggc gatccggcgc gcgcgcggct cgagcttgtg cagccacagg
     1921 ccgacgcgca ccagctcgcc gtggcggaac gtgtgaccga gcgcgagaaa ggcggacgcg
     1981 gcgcaaagcc aggccacgat gtcgtcggcg ccgcggatca gcacgccggc ggtgcggccg
     2041 aatgcctgcg ccagcatcac cacgcagatg gcggcgaggc agatggcggc gagaacgccg
     2101 cttgcggcgt agaggcggtc taaaaaccgc ctcaagaggt tatttgcctc tgtacgcttt
     2161 gatgatcgac tgaccttctg ccccggactt cttcagccac tcgtccagga tctggtggcc
     2221 gaccttggcg tacgcgtcct gcatcgctgc gtcgggcttg tggatggtca tgccgttctt
     2281 ggcgagcagc tcgttcgcct cgagcgtgcg caccttcgac atgccccagc cgcgcgtctc
     2341 ggcggtagac gccgcgcgca tgatcgcctg ctgcacgtcc gccggcaggc gcgagaaccc
     2401 cttggtgttc accacgaccg cgtttttcgg atggaacgcg ctcgtgtcgt agtagttctt
     2461 gacgaactcc catgccttgg tggaagtgcc ggtcgcggac gaggtgatca tcgcctggat
     2521 gccgccggtg gcgaacatct gcggcacgtc gggcacctgc accaccgtcg ggctcgcgcc
     2581 gagcagctcg gcgagccgcg cagtgagcgg gctgtaggtg cggaacttcg tgcccttcag
     2641 gtccgccacg ctcttcagcg gctccttggt gtagatgcct tggcccggcc aggcgaccga
     2701 gtagagcagg gtcaggccgc gcccctgcag cttcttctcg agcagcggct tctgcgcctg
     2761 gtagaacttc caggcgtcgt cgtagcccgc ggcgacgaac gggatgttgt cggcctcgaa
     2821 caccgggtcc tcgttgccga actgcgagag caggaattcc gcgatgttca cctggccggt
     2881 agagaccgcg cgcaggatgt cgggatgctt gatgagcgag ccgttcgagt gcaccacgat
     2941 ctcgagcttg ccgccggcgg ccttctgcac gctctcgacg aactgcttga cgttgcgggt
     3001 atggaactcg ccctcggaat acggcgtggg catgtcccat ttggtctgtg cctgggcgcc
     3061 gagcgagagg gcggcgatcg cgaacagcaa gctccggagc atggggttcc ccctatggac
     3121 gattgtgcat cttagctcag ttcattcagc acgatggcgg cgagcgcgag gctcgaggtg
     3181 agcccgggcg actcgatgcc gaagagctgc accaggcccg gcacgccgtg cacgcgcggt
     3241 ccctgcaccg cgaagtccgg cgccggctcg ccggggccgg cggtcttggg ccggattccg
     3301 gaatagcccg gcgccagcgc gccgtcgggc aggccgggcc agtagcgccg gatcgcggca
     3361 tagaagcgct cggcgcgctt cggatcgacg gcgtagtcga tgcgctcgac ccattccacg
     3421 tccgggccga agcgcgcctg cccggcgagg tcgagcgtca cgtgcacccc gagcccgccg
     3481 ggctccggca ccggatagac gaggcgcgag aagggcgcgc gccgcgcgag ggaatagtag
     3541 ttgcctttgg cgtagtactc gcccggcgct ttgtgttcgt aaccttgaat gaggcgagcg
     3601 acgctgggtg ccctgaggcc ggcgctattg acgagcacgc gggcccggat cggatccgca
     3661 ccgtcgacgc gcacgacgaa gccgtctttc tcgaccgtgg ctttctcgaa gcggcttttc
     3721 agcgcgagca tcgcgccccg gtcctcggcg tcgccgaggt aggcgagcat cagcgcgtgg
     3781 ctgtcgatga tgccggtgga gggcgaatac agcgcggcca cgcaggaaag ctcgggctcg
     3841 agcgcgagcg cctggctgcg ggtcatccac acaatgtcgg taacgccgtt ggcgtgcgct
     3901 ctgcgttgta tagagagaag ttcctcgttc tggctctccg aggtagaaac gatcagcttg
     3961 cccgagcgcc ggtgcggcac gccgtgcgcg acgcaatact cgtacagcag ctctttaccc
     4021 gcgacgcagc tgcgcgcctt cagggagccc ttcgggtaat agatgccggc gtggatcacc
     4081 tcggaattgc gcgagctggt gtgcgtgccg atcgcgtcct cggcctcgag gatcacgacc
     4141 tcgcgccccg cccgggcgag ctcgcgcgcc accgccagcc cgaccacgcc gccgccgatg
     4201 acgaccgcgt caacgctttc cacggaccag gaaccgctcg aacaagaacg ccgcgacgcc
     4261 cgcgagcgcc gcgaccgtcg ccatcggccg ggcgctgccg tcgaacgccg cgccgagccc
     4321 ggcgctgatc agtgctccgg cggtgaacgc gatcgcgccg atcagcgacg aggccgagcc
     4381 cgccgccttg ggaaacggcg aaagcgccga cgcggtggca ttcggcacgg tcagcgcgct
     4441 gcccaggagg aacgcgaaga acggcgccac gacggcggcc cagtgggcga ccccgcccca
     4501 ggcgagcgcc gccgcggcga gcccgcccgc gagcatcagc gcggcgcccg agcgcagcag
     4561 cgtgacgatt cccaggcgca gcacgaggcg gctggagcac caggcgcccg agatctggcc
     4621 gagcatgacc gcagcgaaca tcacgctgta ggcgccgacc gacacgccca ggccgctcac
     4681 cagcgtgaag gccgagttgg agacccaggc gaggatgccg atctgcgcgc acaggatgag
     4741 gaggaacggc gccacgaagc gccgctcggc gaggatcgtc cggaaggtgc gcgcgatgtc
     4801 cgcggggtgc gccgagcgcc gctcgccggg cgcggtttcg cgcaggccgg cgccggtcgc
     4861 gactatgagg agcgccgccg ccagcgccag gcaccagaac accgcctgcc agccgccgct
     4921 gccgacgaga agcgcgccgc cgagaggcgc ggcgatcggc acgatgctga agacgatggt
     4981 gatgctggcg aggagccgcg ccgcctgctc gtgcgcgtag aggtcgcgca cgatcgagcg
     5041 cacgatgacc gcgccgctcg aggtcgccag tccctggacc aggcgcgccg cggtcaccgc
     5101 gccgaccgag tccaccgccg ccgcagcgag cgaagacgcg agcagcagga aaagtcccgc
     5161 cagcagcacg ggcttgcgcc cgaagcggtc ggagagcggc ccccacagga gctgccccat
     5221 ggcgaggccg aggaagtagg tcgtgagcgt gagctgcgcg gcggcgacgt ccgcggcgag
     5281 cgcttgcgcg agcaccggca gcgccggaag gaaaagatcc gtgccgagcg cgggcagcgc
     5341 catgagcacg cccagcagca ccgtgagacg gagggaagtt tcctgcttgt tcaagttagg
     5401 atgcggattc taaatgcagg ccacgctcgc caactggcgc accgcgcctt tcaaccgctg
     5461 ggcattccat catgtgcgcg agctggtgcc gtcggccgac atcccgcacg atcccgcgcg
     5521 cgcgcgccgg ctgccgcagc acgccgccga tctgccctcg gatttcgagg tatttctgcg
     5581 cgagaccgac accgatggca tcgccatcct gcatcgcggc aagctgatct tcgagcgcta
     5641 cgccaacggc atgaccgagg agacgccgca cattctcatg tcggtgtcga aatcgctgct
     5701 tgcgcttctc ttccacgagc tgggcctcga ttctgcgctg cgcgtgccgc aggtggtccc
     5761 cgagctcgaa agcaccgcct accgcgacgc cacgctgcgg gacctgctcg acatgcgcgc
     5821 cgggctcgtc tgggacgaga actacctcgc cacctccggg ccgatcgtcg aatatcgcaa
     5881 ggcgacgggt tggaatccga cggcgagcgg cgaaaccgcg tcggatctca agtcgttcta
     5941 ccgcaccctg agggaaaccg gcccccacgg cggccgcttt cactatgtat cgccgaacag
     6001 cgaccttctc ggctgggtga tcgagcgcgc gaccggcgcg cgctatgccg aactgatgag
     6061 cgagcgcgtc tggaagaagg tgggcgcgga gcgcagcgcc tacatcacgg tcgatcgtct
     6121 cggcgcgccg cgctgcgctg gcggcatgtg cgccacgcta cgcgacctgg cgcgcgtagg
     6181 gcagtggctg atcgaaagcc ggcgcgggtt cctcgaggac ctcgagcgcg gcgacgcgaa
     6241 ggcgtgggcg gccggggacc tcgcgcacta ctaccccggg ctgccgatgt gctaccgcaa
     6301 ccagtggtac gtgctggacg caaatagggg caagagcgca caggatgtga atgccgcacc
     6361 cctcgtcttc ggcatgggga tccacggcca gaacctgttc gtcgaccgcg cgaacgagat
     6421 cgtgatcgcc aagttctctt cccaggaggc gccgctcgac ccggtgcgca tttcgctcac
     6481 gatgcgcatg gtcgcgttgc tgaggaaacg ctttgcctga gcgctggcaa ttctggatcg
     6541 accgcggcgg cacgttcacc gacgtggtcg cgcgccggcc cgacggcgcg ctggcgacgc
     6601 acaagctgct gtcggagaac cccggacgct acgacgatgc cgcgctcgcg ggcattcggg
     6661 agattttggg tgtgagttcg gatcggcctc tgccggttca cctcattgaa gcggtcaaga
     6721 tgggcaccac ggtggcgacc aacgcgctcc tcgagcgcaa gggtgagcgc acggcgctgt
     6781 tcatcacgcg cggattccgc gacgcgctgc gcatcgccta ccagaaccgg ccgcgcatct
     6841 tcgaccgcca catcgtgctg ccggaaatgc tctattcgaa ggtcgtcgag gtcgaagagc
     6901 gcgtcgcggc gcgcggggag gtggtcgttc ccctgaacga ggacgacgca cgcacccggt
     6961 tggcggaaac ctacaacgaa ggctatcgct cgatcgcgat cgtgttcatg cacggctacc
     7021 gcttccccga gcacgaacgc aggacggccg agcttgcgaa agccgcgggc ttcacccaga
     7081 tctcggtctc gcaccgggtc agcccgttga tgaagctggt gggacggggc gacaccacgg
     7141 tggtcgacgc ctatctctcg ccgatcctgc gccgctacgt cgagcaggtc gccgccgaat
     7201 tgcgcggcgt gcggctgctc ttcatgcagt cgaacggcgg cctggcggat gcacggcgct
     7261 tccaggggaa ggacgcgatt ctgtccggcc ccgcaggcgg catcgtcggc gcggtgcgca
     7321 cctcggcggc ggcgggcttc gaccgcatca tcggtttcga catgggcggc acctcgaccg
     7381 acgtgtcgca ctacgcgggc gagctcgagc gcgagttcga gacccgggtc gccggggtgc
     7441 gcatgcgcgc gccgatgatg agcatccata ccgtcgcggc gggcggcggc tcgatcctgc
     7501 atttcgacgg cgcgcgctac cgcgtcggcc ccgattccgc cggggcgaat cccgggccgg
     7561 cgagctaccg ccgcggcggg ccgctcaccg tcacggacgc gaacgtcctg ctcggcaaga
     7621 tccagccggc gcattttccc gcagtctttg gttcttccgg cagggagtct ctcgaccggg
     7681 aggtagcgac gggaaaattt ctggaattgc agaagagcat cggcgatggt cgctcggtgg
     7741 aag
//
LOCUS       DATBSZ010000095         3929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_218578, whole genome shotgun sequence.
ACCESSION   DATBSZ010000095 DATBSZ010000000
VERSION     DATBSZ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218578"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VIG70_06190"
     CDS             <1..483
                     /locus_tag="VIG70_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250177.1"
                     /translation="IVAGLMKDAHQARGLIRALADAAFEREEIDMSGGPVVGLVARGI
                     PEGEAQIFAEGIRRGGAVVAVKADDEMEAEQAALLMNRHGAVDLEACGTGWRSQGWSG
                     RITAPEDRVTIERYAYVFGEYPAGAGMIYRDRRARASYRGPERRHIDKSYAGINRRAA
                     "
     gene            complement(464..1558)
                     /locus_tag="VIG70_06195"
     CDS             complement(464..1558)
                     /locus_tag="VIG70_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006479109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8250178.1"
                     /translation="MGAAPRAFRIQIPDAALADLHARLDRTRFPDEPPLEAWSTGTSL
                     PYLKELIAYWRSGFDWRAQEAKLNRFAQYEVSLAGIDLHFIHEPGRGPKPMPLLLMHG
                     WPGSVWEFHRLIPLLTEHFTVVAPSLPGFTFSFKPGQPRFGVEEMADAFAELMTGVLG
                     YSRFGVQGGDWGAFIAARLGYAHARHLAGIHLNYLPVRREPKLSDDPGWAKQLAHWAA
                     EESGYIGIQGTKPQTLAYGLSDSPAGLAAWIAEKFRTWSDEPIGRDDLLTNVSLYWFT
                     GAIGSSFWPYYARAHRGWPIPEGAAVAVPVGYAEFPKEILRPPRSLAEKTYTRIERWT
                     SMKKGGHFAALEQPEALAPEIVSFFRPLAG"
     gene            1579..1767
                     /locus_tag="VIG70_06200"
     CDS             1579..1767
                     /locus_tag="VIG70_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250179.1"
                     /translation="MPTAQQFICKKCRSLVTIETFQHSRDGTPYCPCPNCGAKNAVVE
                     AGLTPSQPGILPVTRLLD"
     gene            1894..2133
                     /locus_tag="VIG70_06205"
     CDS             1894..2133
                     /locus_tag="VIG70_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250180.1"
                     /translation="MDKRKVWREQEQQLVEKWNSAAQRYRDIMAQIAREDPFATPGGP
                     SEEIMSKAAAVRAEIDTLRKKVARLKAEFNTGKRY"
     gene            2145..2357
                     /locus_tag="VIG70_06210"
     CDS             2145..2357
                     /locus_tag="VIG70_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250181.1"
                     /translation="MRPGRSAAWTRERIDLLTTPEIRQLRDNADRLGESEIVGLCEEI
                     LKVRPKTGSGLKNVKTAVKAKRPAAA"
     gene            2354..2692
                     /locus_tag="VIG70_06215"
     CDS             2354..2692
                     /locus_tag="VIG70_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250182.1"
                     /translation="MSIPTVERRRFKVGKYDIIANPIPNSAHMLRYTVFVAGKRIGAT
                     VSVPTESDCRFLEKPPIVPPLKIFSSYRPGRPKKGSRPPQPAESAGHQTREALPLDVS
                     LGGISATTDR"
     gene            complement(2716..3681)
                     /locus_tag="VIG70_06220"
     CDS             complement(2716..3681)
                     /locus_tag="VIG70_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250183.1"
                     /translation="MAKKRAPAKRRPRKAKPGTRGLTPAETRLESLSGEAQAVSERVE
                     QVGGHVLGCYNDPLGKQPVLVAILPIESVEPTPFQRDLSQVHHRKLADVLDRTGMFLD
                     PIIAVTAPEKGFWTPNGMHRLMAMRRLGARAITALVVPKREIAWQILALNTEKAHNLR
                     EKALEVIRIYRGLLGEDAGRTESGFSFYLEEASLVTLGICYEKNGKFAGGAYNPIVRR
                     LEKFSEEAISKAIRIHEKHAGLLMELDERVTEAVKKLKEKGFISPYLRTFVVARINPL
                     RWIQGEPPPLEEVLKTMRDRAARFNVDKIKQSDLANMGGAPGDQD"
BASE COUNT          682 a         1306 c         1304 g          637 t
ORIGIN      
        1 atcgttgcgg gcttgatgaa ggacgcccac caggcgcgcg gcctgatccg cgccctcgcc
       61 gacgcggcgt tcgagcgcga ggaaatcgac atgagcggcg gcccggtcgt ggggctggtc
      121 gcccgcggaa ttcccgaggg cgaggcgcag atcttcgctg aaggcatccg ccgcggcggc
      181 gcggtcgtcg cggtgaaggc cgacgacgag atggaagccg agcaggccgc gctgctgatg
      241 aaccggcacg gcgccgtgga cctcgaggcg tgcggaaccg gctggcgcag ccaggggtgg
      301 agcggccgca tcaccgcgcc cgaggatcgc gtcacgatcg agcgctacgc gtacgtattc
      361 ggggaatacc ccgcgggcgc ggggatgatc taccgcgacc ggcgcgcaag ggcttcgtac
      421 cgcgggccgg agcgccggca tatcgacaag tcgtacgccg ggatcaaccg gcgagcggcc
      481 tgaaaaaact cacgatctcc ggggccagcg cctcgggttg ctcgagggcg gcgaaatggc
      541 cgcccttttt catgctcgtc cagcgctcga tgcgcgtgta ggtcttctcg gcgagcgagc
      601 gcggcggccg caggatctcc ttggggaact cggcgtagcc cacgggcacg gcgacggctg
      661 cgccttcggg gatcggccag ccgcggtgcg cgcgcgcgta gtagggccag aacgacgagc
      721 cgatcgcccc cgtgaaccag tagagcgaaa cgttcgtgag caggtcgtcg cggccgatag
      781 gctcgtcgct ccaggtgcgg aatttttcgg cgatccacgc ggccaatccg gccggcgagt
      841 cgctcaagcc gtacgcgagg gtctgcggct tcgtgccctg gatgccgatg tagccgcttt
      901 cttcggccgc ccagtgcgcg agctgcttcg cccagccggg atcgtcggag agcttcggct
      961 cgcgcctgac cggcaggtag ttgagatgga tgcccgcgag gtgtcttgcg tgcgcgtagc
     1021 cgaggcgcgc cgcaatgaac gcgccccagt cgccgccctg cacgccgaag cgcgagtagc
     1081 cgagcacgcc ggtcatcagc tcggcgaacg cgtcggccat ctcctccacg ccgaagcgcg
     1141 gctggccggg cttgaagctg aaggtgaatc ccggcagcga cggcgcaacc acggtgaaat
     1201 gctcagtgag aagcggaatg agccggtgga attcccagac cgatcccggc cagccgtgca
     1261 tcagcaggag cggcatcggc ttcggtcccc gccccggctc gtggatgaaa tgcaggtcga
     1321 tgccggccag ggacacctcg tactgtgcaa agcgattcag cttcgcttcc tgcgcgcgcc
     1381 agtcgaaccc ggaccgccaa tacgcgatca gctctttcag ataaggcagc gaggtgcccg
     1441 tggaccaggc ttcgagcggc ggctcgtcgg gaaagcgcgt gcgatcgagg cgggcgtgca
     1501 ggtcggcgag cgccgcatcg ggaatttgaa tgcgaaaagc cctcggagcg gctcccatcc
     1561 gcacagtatg attgccgcgt gcccacagca cagcagttca tctgcaagaa gtgccgatcg
     1621 ctcgtgacga tcgaaacctt tcagcactcc cgagacggca cgccctactg cccctgcccc
     1681 aactgcggcg cgaaaaacgc ggtcgtcgaa gccggcctta cgccgagcca gccgggcatc
     1741 ctgccggtca cccggctgct ggattaaggg cttacgtaag ttccgaattt tgttcagtaa
     1801 tgtgccggta ctgtggtaaa cttcacgcct tactccgcag tttcctccgg ttcctcccaa
     1861 acgtccgctc cggcgggcgc gaaaaggaca ttcgtggaca agcgcaaggt gtggcgcgag
     1921 caggagcagc aactggtcga gaagtggaat tccgccgcac agcgctaccg ggacatcatg
     1981 gcccagatcg ctcgcgagga tcccttcgcc acgcctggcg ggcccagcga ggaaatcatg
     2041 agcaaggcag ccgcggtacg cgccgagatc gataccctgc ggaagaaggt ggcgcgcctg
     2101 aaggcggagt tcaacacggg aaagcgatac tgacgatgag acttgtgaga cctggacgtt
     2161 cggcggcctg gacccgagaa cggatcgacc tgctgacgac gcccgagatc cgccagttgc
     2221 gcgacaacgc cgaccggctg ggcgagtcgg agatcgtcgg gttgtgcgag gagatcctca
     2281 aggtgcggcc caagacgggc agcggcctga agaacgtcaa gacggccgtc aaagccaagc
     2341 gcccggcggc ggcatgagca tccccaccgt cgagcgccgg cgcttcaagg taggcaagta
     2401 cgacatcatc gccaatccca ttccgaattc cgcgcacatg ctgcggtaca ccgtattcgt
     2461 cgccggcaag cgcatcggag cgacggtgag cgtgccgacc gagtccgact gccgcttcct
     2521 ggagaagccg ccaatagtgc cgccgctcaa gatcttcagc agctaccggc ccgggcgccc
     2581 gaagaaaggc tcccgcccgc cgcagcccgc cgagagcgcc ggacaccaga cgcgcgaagc
     2641 cctgccgctc gatgtctcgc tcggcggaat tagcgcgacg accgaccgct agcaaccccg
     2701 cagcaaagac cgcgctcagt cctgatcgcc gggcgcgccg cccatgttgg cgagatcgga
     2761 ctgcttgatc ttgtcgacgt tgaagcgcgc cgcgcgatcg cgcatggtct tcagcacttc
     2821 ctcgagaggc ggcggctcgc cctggatcca gcgcagcggg ttgatgcgcg cgacgacgaa
     2881 agtccgcagg tagggactga tgaaaccctt ttccttcagc ttcttcactg cctcggtaac
     2941 gcgctcgtcg agctccatca gcaggccggc gtgcttttca tggatgcgga tcgccttgct
     3001 gatcgcttcc tcggaaaact tctccaggcg cctcacgatc gggttgtagg cgccgccggc
     3061 gaactttccg ttcttctcgt agcagatccc gagcgtcacc agcgaggctt cctccaggta
     3121 gaaggagaac cccgactcgg tccggcctgc gtcctcgccg agcagacccc gatagatgcg
     3181 gatcacctcg agcgccttct cgcgcaggtt gtgcgccttc tcggtgttga gcgcgaggat
     3241 ctgccacgcg atctcgcgtt tgggcaccac cagcgccgtg atcgcgcgcg cccccaggcg
     3301 ccgcatcgcc atcaggcggt gcattccgtt cggcgtccag aagcctttct cgggggcggt
     3361 gaccgcgatg atcgggtcga ggaacatccc ggtgcggtcg agcacatcgg caagcttgcg
     3421 gtggtggacc tgcgagaggt ccctctggaa cggtgtgggt tccacgctct cgatcggcag
     3481 gatcgccacc agtaccggct gcttgccgag cggatcgttg tagcagccca gcacgtgccc
     3541 gcctacctgc tcgacccgct cgctcaccgc ttgcgcttct ccgctcagac tctcgaggcg
     3601 cgtctcggcg ggggtcaggc cccgcgtccc gggctttgcc ttgcgcggcc ggcgcttcgc
     3661 gggggctcgt ttcttcgcca tgcgcaaatt atctcaagac ggtaggcagg caaaaaaagt
     3721 gatatgcgcg ggcacccctt cccccgtagg tacgcgagtc gcatatcacc aaaaaaagaa
     3781 gcgggcctcg aaggcccgct agcttccgtc gccggctagt tcgccgggtg ggcctggtcc
     3841 aggcccgtca gcctgtccgg ctcgtagccc tgcttcttcg cctcgcgctg ctcgtcgccc
     3901 gacgagggcg tggcgcccag cgcgctttc
//
LOCUS       DATBSZ010000096         7958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1380043, whole genome shotgun sequence.
ACCESSION   DATBSZ010000096 DATBSZ010000000
VERSION     DATBSZ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380043"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VIG70_06225"
     CDS             <1..258
                     /locus_tag="VIG70_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEY8250184.1"
                     /translation="WTELDIWRYCQRERIPCVELYFSKNGKRYRSLGDQDITSPVASN
                     ASTLEEILAELETTKVSERSGRKMDHESEDAFERLRADGYM"
     gene            267..2132
                     /gene="cysC"
                     /locus_tag="VIG70_06230"
     CDS             267..2132
                     /gene="cysC"
                     /locus_tag="VIG70_06230"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR02034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY8250185.1"
                     /translation="MKLVIVGHVDHGKSTLVGRLLHETGALPDGKVAAVKASSEKRGM
                     PFEWAFVIDAMQAERDQGITIDTTQIRFKTAARDYLLIDAPGHKQFLKNMVSGAAGAD
                     AVILVVDAHDGVQDQTRRHAYLLRLLGIRRIIVAINKMDLVGYREERYQEVRRDVAAY
                     LRSIGVNAVDINFLPLAARDGENMRSRGTMPWYEGPTLLEALDAVPAPLAATDLPLRL
                     TVQDVYKFDHRRIIAGRIESGSLRVGDTLLFSPINATARVASIEAWNAPAPAKAGVGQ
                     SVGLTLDEDIFVERGHVASHAHSAPVVSNVFRANLFWLSRSPLVPGAQYRLRRGAAET
                     LAVVERIDRVVDTNTLASGPGEKVEWNGVAEVTIRTRGLLALDHHADNALTGRFVLVD
                     GHLIVGGGTASTEGYPDQRRGTAPKAANLFSVRHRISRDDRARLSGHRGGILWFTGLS
                     GSGKSTLAVELERVLHAKGYQVFLLDGDNVRQGLCADLGFSPQDRTENIRRVGEVSAL
                     FAEAGMIVVSAFISPYRVDRDRIRGAHGDVFHEVYINAPLHVCESRDVKGLYKKARAG
                     QVKEFTGISAPYEAPVAPELEIPSGEWTPERCIATLSEYVERRLAYRPAKIHAVK"
     gene            2132..3082
                     /locus_tag="VIG70_06235"
     CDS             2132..3082
                     /locus_tag="VIG70_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEY8250186.1"
                     /translation="MRRIFVFNGDADGLCALQQLALAEKPVSMELVTGPKRRTELLAR
                     VTAGAGDEATVLDVSFHANREGVQRLLAAGARVRYFDHHHAGLLPSHERLEAHIDLLP
                     STCTSIIVNRYLDFKYKAWAAVGAYGDNLADTGRSLVDSRELEKLGTLLNYNAYGETE
                     ADLFFPPAELHRRLAPYADPLAFIGEDAAYQVLADGYAQDMQRAADLKPVAARGNAAV
                     YVLPDASWARRVSGALANRLARSAPARAHAMLSPNRAGGMQVSVRAPLARPSGAAALC
                     LDYATGGGREAAAGINHLPMESVGDFTDRFIRYFDRGSTG"
     gene            complement(3048..4022)
                     /locus_tag="VIG70_06240"
     CDS             complement(3048..4022)
                     /locus_tag="VIG70_06240"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8250187.1"
                     /translation="MKIRKPDLSSTPLNQEHEQHGLPPAEQIAATAAAHPVLRTATWF
                     TAITLTALTIYLVARLFLPNNWVAGLQTIATTLEGSPFWTAVAVGLLAQAVDGALGMA
                     YGVTASTFLLGMGASPAVASASVHIAEIFTTGVSGMAHVKYGNVNRKLFLRLLVPGVL
                     GAVLGVWVVTQIDGKVLKPYISAYLLIVGLYILSKAFRHLRARQREPRHVAKLAVFGG
                     FVDSVGGGGWGPVVTSTLVGSGNDPRTTIGSVNFAEFFLTLATAASFTLLAVEGTWPV
                     VAGLVVGGLFAAPFAALLVKKLSTRTLLISVGVLITALSLWNLYRSIV"
     gene            4170..5684
                     /locus_tag="VIG70_06245"
     CDS             4170..5684
                     /locus_tag="VIG70_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250188.1"
                     /translation="MRFLRTLALVAVPLAANAAPSAVSTFHSIGLYWSPEGGSESNAA
                     RVEFRASGSAEWRQGLDLWFDARNREYRGSLVELEPGTEYEVRLALSSGGVESMKTRT
                     WSERFPVKRTVDVSGKKRVTIGPEDSGDEKGYVVFTAPPGRNVIEGNDTDESCVVVRQ
                     GAHHVIVRGLELRNCKRYGVLIERQAQREGQPALDAQTHDIVIEGNEIAGWGGFGNLP
                     SAKDLADTDGAIHCNYYRETEDARRPDRIVIQGNRIRDPRHGSHPWVSAKHGRKHPKG
                     PLGVMFNRCGSNHVIRYNDISSKNGNHYEDGLGGADNFSAAGFPWADSDIYGNRIANV
                     YDDGIEAEGGNRNVRIWGNHLERVNVAIANAATTTGPLYVWRNVSANTQGMYDPEGRE
                     RRRQDGPFIKAGSRDATANGGRAYYFHNTGMGAGAGIARSGGRAYNFVARNNQWPKSR
                     VDPADGPFDLGKDRVERIANFNDRYARPESGAHQSGAPPMRFGAEAYTAGRSAD"
     gene            complement(5581..6393)
                     /locus_tag="VIG70_06250"
     CDS             complement(5581..6393)
                     /locus_tag="VIG70_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY8250189.1"
                     /translation="MALYGLIAGIQGNREALSAALEALERHGADRVVCLGDVVGYNAD
                     PEECVALVRGRCASAIAGNDDLIGVRQLDFRRCSTKAEYSLRRTRRALSPESRAWLAA
                     LPAVRILQEGVILVHGGVRDVEQPMASAEHIRGNAQLLREDFPGARLCFYGHGHEQKV
                     YRVEGNEAVELPGDRHSLEENHLYFVSPGSVDASRKRGPRLAECALFDSFDRTIDFLR
                     VPYDSAATEAKAIVFGYRINPLTDRLYTLRRRTASAARRIGERLTRAAHTGR"
     gene            6560..7291
                     /locus_tag="VIG70_06255"
     CDS             6560..7291
                     /locus_tag="VIG70_06255"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HEY8250190.1"
                     /translation="MKVAIHQPHFLPWLGYLHRMAQADLFVLLDHVQFERRNYQNRTM
                     IRMNGEARWLTVPVIQRSRDERIADKEVDNRLDGAKWWSPNHFTTLRHAYRDARFFDT
                     YAAEFRRLFDTRWDKLVDLNQAGLDLLRDAFGIRTPLVRSSEFTVEGTRMELILNVCR
                     AAGADTLIAGMGGSRGYLDADAFARAGVRIEYHDFKHPEYPQGGRGAPQAVPFIKGLS
                     AIDMLFNCGPQASSLLLPDRMAKAA"
     gene            7288..>7958
                     /locus_tag="VIG70_06260"
     CDS             7288..>7958
                     /locus_tag="VIG70_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY8250191.1"
                     /translation="MNAPLPARAFQTRSISLASRYGGRTVLAIGAHPDDLELAIGGTL
                     ARLARESARVVMAVVSIPADFAMRRAEAQRAAEILGCELRILVDDDCCRVDDIRNHQL
                     VGMLDALVRSVKPAAVFTHSASEFHRDHVVVHNACLSTQRLEPFDFFQFSPTMTRAVP
                     VAFHPRAYVDVSSTIDAKMEAIAAHASQFGTRGLDAEMFRDIARLNGRMVGVKYAEGL
                     DVGRML"
BASE COUNT         1325 a         2773 c         2694 g         1166 t
ORIGIN      
        1 tggaccgagc tcgacatctg gcgctactgc cagcgcgagc gcattccctg cgtggagttg
       61 tacttctcga aaaacgggaa aaggtatcgc tcgctgggcg accaggacat cacctcgccg
      121 gtggcgagca acgcctcgac cctcgaggag atcctggccg agctcgagac caccaaggtg
      181 tcggagcgct ccggccgcaa gatggaccac gagtccgagg atgcctttga acgcctgcgc
      241 gcagatgggt acatgtgatg agtcacttga aactggtgat cgtcggtcac gtcgaccacg
      301 gcaaatccac gctggtgggc cgcctgctgc atgaaaccgg cgcgctgccc gacggcaagg
      361 ttgccgcggt gaaggcctcg tccgaaaagc gcggcatgcc cttcgagtgg gcgtttgtga
      421 tcgacgcgat gcaggccgag cgcgaccagg gcatcaccat cgacaccacg cagatccgct
      481 tcaagaccgc ggcgcgcgac tacctgctga tcgacgcccc gggacacaag cagttcctga
      541 agaacatggt gagcggcgcg gccggcgccg acgccgtgat cctggtggtc gacgcgcacg
      601 acggcgtgca ggaccagacg cgccgccacg cctatctgct gcgtctgctc ggcatccgcc
      661 gcatcatcgt cgccatcaac aagatggacc tggtcggcta ccgcgaggag cgctaccagg
      721 aggtgcggcg cgacgtcgcc gcctacctgc gctcgatcgg cgtgaacgcc gtggacatca
      781 acttcctgcc gctcgccgcg cgcgacggcg agaacatgcg cagccgcggc accatgccgt
      841 ggtacgaggg cccgacgctg ctcgaggcgc tcgacgcggt gccggcgccg ctcgccgcca
      901 ccgacctgcc gctgcgcctc acggtgcagg acgtctacaa gttcgaccac cgccgcatca
      961 tcgccggccg catcgagagc ggcagcctgc gcgtcggcga cacgctgctg ttctcgccga
     1021 tcaacgccac cgcccgggtt gcttccatcg aagcctggaa tgcgcctgcc cccgcgaagg
     1081 cgggggtcgg ccagtcggtc ggcctgacgc tggacgagga catcttcgtc gagcgcggcc
     1141 acgtcgcgag ccatgcgcac agcgcgccgg tggtgagcaa cgtgttccgg gcgaacctgt
     1201 tctggctctc gcgctcgccg ctcgtgccgg gcgcgcagta ccgcctgcgc cgcggcgcgg
     1261 ccgagaccct cgccgtggtc gagcgcatcg accgagtggt cgacaccaac acgctcgcct
     1321 ccgggccggg cgagaaggtc gagtggaacg gcgtcgccga ggtgacgatc cgcacccgcg
     1381 gcctgctcgc gctcgaccac cacgccgaca acgcgcttac cgggcgcttc gtgctggtcg
     1441 acggccatct catcgtcggc ggcggcaccg cgagcaccga gggctacccc gaccagcgcc
     1501 gcgggacggc gccgaaggcc gccaacctgt tctcggtccg ccaccgcatc tcccgcgacg
     1561 accgggcgcg cctgagcggc caccgcggcg gcatcctctg gttcaccggc ctctccggct
     1621 cgggcaagtc gaccctcgcg gtggagctcg agcgcgtgct gcacgccaag ggctaccagg
     1681 tgttcctgct cgacggcgac aacgtgcgcc aggggctgtg cgcggacctc ggcttctcgc
     1741 cgcaggaccg caccgagaac atccgccgcg tcggcgaggt gtcggcgctc ttcgccgagg
     1801 ccgggatgat cgtggtgtcg gccttcatct cgccctaccg cgtcgatcgc gaccgcatcc
     1861 gcggcgcgca cggcgacgtg ttccacgagg tgtacatcaa cgcgccgctc cacgtctgcg
     1921 agtcgcgcga cgtgaagggc ctctacaaga aggcgcgcgc cggccaggtg aaggagttca
     1981 ccggcatctc ggcgccgtac gaggcgccgg tcgcgcccga gctcgagatc ccgagcggcg
     2041 aatggacccc ggagcgctgc atcgccacgc tgagcgagta cgtcgagcgg cgtctcgcgt
     2101 accggccggc aaagatccac gccgtcaagt agtgcggcgc atcttcgtct tcaacggcga
     2161 tgcggacgga ctctgcgctc tgcagcagct ggccctcgca gaaaagccgg tttcgatgga
     2221 gctcgtcacc ggccccaagc gccgcaccga gcttctcgcc cgcgtaaccg ccggcgccgg
     2281 cgacgaggcc accgtcctcg acgtttcgtt ccatgccaac cgcgagggcg tccagcggct
     2341 gctcgccgcc ggcgcgcgcg tgcgctattt cgaccatcac cacgcgggcc tcctgccgag
     2401 ccacgagcgg ctcgaggcgc atatcgatct cttgccctcg acctgcacca gcatcatcgt
     2461 caatcgttat ctggatttca aatataaggc gtgggcggcg gtcggcgcct atggggacaa
     2521 cctcgccgac acgggaagat ccctggtcga ttccagggaa ctggaaaagc tcggcaccct
     2581 gctcaattac aacgcttacg gcgagaccga ggcggacctt ttcttcccgc ccgccgagct
     2641 gcatcgccgc ctcgcgcctt atgccgatcc cttggctttc atcggggaag acgccgccta
     2701 ccaggttctg gcggacggct atgcgcagga catgcaacgc gcggcggacc tgaagccggt
     2761 agcggcgcgc gggaacgccg ccgtctacgt gctgccggat gcgagctggg cgcggcgcgt
     2821 gagcggggcg ctcgcgaacc gcctggcgcg ctcggcgccc gcgcgcgcgc atgcgatgct
     2881 cagcccgaac cgcgccggcg gcatgcaggt cagcgtgcgc gcgccgcttg cgcggccctc
     2941 gggcgcggcg gcgctatgcc tggactatgc caccggcggc gggcgcgaag ccgccgccgg
     3001 gatcaatcac ctgccgatgg agagcgtggg cgatttcacc gaccgcttca tacgatactt
     3061 cgatagaggt tccacaggct gagcgcggta atcagcacgc cgaccgagat cagcagcgtg
     3121 cgcgtgctca gcttcttgac cagcagagcg gcaaagggcg cggcgaacag gccgccgacg
     3181 accagacccg cgacgaccgg ccaggtgccc tcgaccgcga gcagcgtgaa cgaagccgcg
     3241 gtcgcgagcg tcaggaagaa ctcggcgaaa ttgaccgagc cgatggtcgt gcggggatcg
     3301 ttgcccgagc cgaccagcgt gctcgtcacc accgggcccc agccgccgcc gccgacggaa
     3361 tcgacgaacc cgccgaagac cgcgagcttg gccacgtggc gcggctcgcg ttgccgcgcg
     3421 cgcaggtgac ggaacgcctt gctcaggatg tagaggccga cgatcagcag gtacgcggag
     3481 atgtagggct tgagcacctt gccgtcgatc tgggtcacca cccacacgcc gagcaccgcg
     3541 cccaggactc ccggcaccag gagccgcagg aaaagcttgc ggttcacgtt gccgtatttc
     3601 acgtgcgcca tgccggaaac gccggtcgtg aagatctcgg cgatgtgcac gctcgcgctc
     3661 gccaccgcgg ggctcgcgcc catgccgagc aggaaggtgc tcgcggtcac gccgtaggcc
     3721 atgccgagcg cgccgtcgac cgcctgcgcc agcagcccga ccgccaccgc ggtccagaat
     3781 gggctgcctt cgagcgtggt cgcgatggtc tgcagcccgg caacccagtt attgggcagg
     3841 aaaaggcgcg ccaccaggta gatcgtcagg gcggtgaggg tgatcgcggt gaaccacgtc
     3901 gcggtgcgca acacagggtg cgcggccgcc gtggcggcga tctgctccgc aggcggaagg
     3961 ccgtgctgct cgtgctcctg gttgagcggg gtgctgctga ggtccggctt gcgtattttc
     4021 aatcggctct cggggaggtt tcggaaggaa acgcatgttc gtcgatttca ttgacattcc
     4081 gattcgccgc gcctagtccg cgtggaacgg cgttggctgg cagcgacgtc aactcgatcc
     4141 cacatgcttg aggccatgag attcagggca tgagattcct gaggaccttg gcgctggtcg
     4201 ccgtgccgct cgccgcgaac gcggcgccga gcgccgtctc cacgttccac tcgatcgggc
     4261 tgtactggag tcccgaaggc ggcagcgagt cgaacgccgc gcgcgtcgag ttccgggcgt
     4321 cgggcagcgc cgaatggcgc cagggcctcg acctgtggtt cgacgcgcgc aaccgcgagt
     4381 accgcggcag cctggtcgag ctcgagccgg gaacggaata cgaggtccgg ctcgcgctgt
     4441 cctcgggagg agtcgagagc atgaaaaccc gaacctggag cgagcgcttc ccggtcaagc
     4501 gcaccgtaga cgtgtccgga aagaagcgcg tgacgatcgg tcccgaggac tcgggcgacg
     4561 agaagggcta cgtggtgttc accgcgccac cggggcgcaa cgtgatcgag gggaacgaca
     4621 ccgatgagag ctgcgtcgtg gtgcgccagg gcgcgcacca cgtcatcgtg cgcggcctgg
     4681 agctcagaaa ctgcaagcgc tacggcgtgc tgatcgagcg ccaggcgcaa cgggagggac
     4741 agccggcgct cgacgcgcag acccacgaca tcgtgatcga gggcaacgag atcgccggct
     4801 ggggcgggtt tggcaacctt ccctccgcca aggacctcgc cgacaccgac ggcgccatcc
     4861 actgcaacta ctatcgcgag accgaggacg cgcggcggcc ggaccgcatc gtcatccagg
     4921 gcaaccgcat tcgtgatccg cgccacggct cgcacccctg ggtctcggca aagcacggcc
     4981 gcaagcaccc gaaggggccg ctcggcgtga tgttcaaccg ctgcggcagc aaccacgtga
     5041 tccgctacaa cgacatttcc tcgaagaacg gcaaccacta cgaggacggc ctgggcggcg
     5101 ccgacaactt cagcgccgcg ggcttcccct gggccgactc cgacatctac ggcaaccgca
     5161 tcgcgaacgt ctacgacgac ggcatcgagg ccgagggcgg caaccgcaac gtgcgcatct
     5221 ggggaaatca tctcgagcgc gtgaacgtcg cgatcgccaa cgccgcgacg accaccgggc
     5281 cgctttacgt atggcgcaac gtgagcgcga acacccaagg catgtacgac ccggaaggcc
     5341 gcgagcggcg ccggcaggac gggccgttca tcaaggccgg cagccgcgac gcgacggcga
     5401 acggcggccg agcctactat tttcacaata cggggatggg cgcgggcgcg ggcatcgccc
     5461 gctccggcgg gcgcgcctac aatttcgtcg cccgcaacaa ccagtggccc aagtcacggg
     5521 tggatcccgc ggacgggccg ttcgacctcg gcaaagaccg ggtcgagcgc atcgcgaact
     5581 tcaacgaccg gtatgcgcgg cccgagtcag gcgctcacca atccggcgcg ccgccgatgc
     5641 ggttcggcgc cgaagcgtat acagccggtc ggtcagcgga ttgatgcggt agccgaatac
     5701 gatcgccttg gcttcggtcg cggcgctgtc atagggaacg cgcaggaagt cgatcgtccg
     5761 gtcgaaactg tcgaacagcg cgcactcggc gagccggggg ccgcgcttgc gcgaggcgtc
     5821 gaccgagccg gggctgacga aatagaggtg attttcctcc agggaatggc gatcgccggg
     5881 cagctccaca gcctcgttgc cttcgacccg gtacaccttc tgctcgtggc cgtggccgta
     5941 gaagcacagg cgcgcgccgg gaaaatcttc gcgcaggagc tgcgcgttcc cgcggatgtg
     6001 ctcggcgctc gccatgggct gctccacgtc gcgcacgccg ccgtgcacca ggatgacgcc
     6061 ttcctgcagg atcctgacgg cagggagcgc cgccagccag gcgcggcttt ccggcgagag
     6121 cgcgcgccgc gtgcggcgca acgagtactc ggccttggtc gagcagcgcc ggaaatcgag
     6181 ctgccttacg ccgatcaggt cgtcgttgcc ggcgatggcg gacgcgcagc ggccgcgcac
     6241 cagcgcgacg cattcctcgg gatcggcgtt gtagcccacc acgtcgccga ggcagacgac
     6301 gcggtcggcg ccgtggcgct cgagggcttc gagcgcggcg gacaatgctt cgcgattgcc
     6361 ctggatgccg gctatgaggc cgtagagcgc catcgcgggt gtcgctcggc agcgacgccg
     6421 gggtcgctgg aatgccgaaa ttccatgcgg gacaggttac gcgggcgctt cggaaatcgc
     6481 ggccccgcag gctgagccaa gcggctcaca cgaggccggt cgatagtcga tgccgctctc
     6541 ggtacgctgg ttccgctcca tgaaagtcgc gatccaccag ccgcatttcc tgccctggct
     6601 cggctacctg caccgcatgg cgcaggccga cctcttcgtg ctgctcgacc acgtgcagtt
     6661 cgagcggcgc aactaccaga accgcaccat gatccgcatg aacggcgagg cgcgctggct
     6721 cacggtgccg gtcatccagc gctcgcgcga cgagcgcatc gcggacaagg aggtcgacaa
     6781 tcgcctcgac ggcgcgaaat ggtggtcgcc gaaccatttc accacgctgc gccacgccta
     6841 ccgtgacgcg cgcttcttcg atacctacgc cgccgaattc aggcgcctgt tcgacacgcg
     6901 ctgggacaag ctcgtcgacc tgaaccaggc cgggctcgac ctgctgcgcg acgccttcgg
     6961 catccgcacg ccgctcgtgc gcagctcgga gttcacggtc gagggcactc gcatggagct
     7021 gatcctgaac gtctgccgcg ccgccggcgc cgacacgctg attgccggga tgggcggctc
     7081 gcgcggctat ctcgacgccg atgccttcgc gcgcgcgggc gtgcgcatcg agtaccacga
     7141 cttcaagcat cccgagtatc cgcagggcgg acgaggtgcg ccgcaggcgg ttcccttcat
     7201 caagggactg tccgcgatcg acatgctgtt caactgcggc ccgcaggcga gctcgctgct
     7261 cctgcccgac cgcatggcaa aggcggcatg aacgcgcccc tgcccgcgag agccttccag
     7321 acccgcagca tctcgcttgc ttcccgctac ggcgggcgca cggtgctcgc gatcggcgcc
     7381 caccccgacg atctggagct cgccatcggc ggcacgctgg cgcgcctcgc gcgcgaatcg
     7441 gcgcgcgtgg tgatggcggt ggtctcgatc ccggcggact tcgcgatgcg ccgcgccgag
     7501 gcgcagcgcg cggccgagat cctcggttgc gagctgcgca tcctggtgga cgacgactgc
     7561 tgccgcgtcg acgacatcag gaaccaccag ctcgtcggca tgctggacgc cctggtgcgc
     7621 agcgtgaagc ccgcagcggt gttcacgcac agcgcgagcg agttccaccg cgaccacgtt
     7681 gtcgtgcaca acgcctgcct ctcgacgcag cggctcgagc ccttcgattt cttccagttc
     7741 agccccacca tgacgcgcgc ggtgccggtg gcgttccatc cgcgcgcgta tgtcgacgtc
     7801 agcagcacca tcgacgccaa gatggaggcg atcgcggcgc acgccagcca gttcggcacg
     7861 cgcggcctcg acgccgagat gttccgcgac atcgcccggc tgaacggccg catggtcggc
     7921 gtgaagtacg ccgagggcct cgacgtcggg cgcatgct
//
LOCUS       DATBSZ010000097         8732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1106524, whole genome shotgun sequence.
ACCESSION   DATBSZ010000097 DATBSZ010000000
VERSION     DATBSZ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106524"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VIG70_06265"
     CDS             <1..804
                     /locus_tag="VIG70_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiB"
                     /protein_id="HEY8250192.1"
                     /translation="PGNVIFLPDHRMVIIDFGMVGRLSPQRRKQVTDLLAGLAHLEER
                     PMLDVLVDWAGDAYVDEAKLAADVNELVFEYQDVPLKDIRIANVVRQFSAIVREHSIV
                     LPADLSMMFKALITLEGLGRQYDPEVHIIDHLAPLLRDALDQRYQPAELVRRGRSALA
                     EFLNLVGSVPRDFARLLREARRGKTRLDLDLKRLDQFGAQVERSINRGSMALMTASLV
                     IGSAIVLNVPNGPTVLGIDVLATLGFAGYAAAFFNSLWVIYGVWRSGRE"
     gene            848..1861
                     /locus_tag="VIG70_06270"
     CDS             848..1861
                     /locus_tag="VIG70_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY8250193.1"
                     /translation="MTDRDRRIVAAWLLVCAALVFAIVLVGGVTRLTRSGLSIVEWQP
                     LVGVLPPLSTADWEALFARYRETPEFRQVNFDMTLESFKGIFWWEYVHRLLARGIGLV
                     FFLPYLVFLMRKKLDRPLAWKLGGIFALGALQGALGWWMVKSGLIDDPKVDPVRLAAH
                     LGLALAIFSAELWIALQLLSPRVRPFETPALLLPGVVFLMALSGGMVAGLRAGSAYQT
                     FPLMNGHLVPPEVLMLEPWWRNFLYNMATVQLVHRAVFWLLAVLVPLAWWRARGTLAG
                     NALLAAFVLQAVLGISTLLLGVPVGLGAAHQGGAVLLLAAALWNAHSAGVWPALRPMH
                     AGA"
     gene            1858..2835
                     /locus_tag="VIG70_06275"
     CDS             1858..2835
                     /locus_tag="VIG70_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250194.1"
                     /translation="MKGYSLPLLVLGFVSLAFGVAGGLARLSALEVPGTAIALHGPLM
                     VSAFFGTVISLERAVALGRPWGYASPLAAGLGGIFLLINFPLTGFALMVLAAAVFVAC
                     GVLVVQRQPSLETATLLAGAGAWLAGNMMLLEGQAAVPWWIAFFVLTIAGERLELSRD
                     LKRPPWVRRAFAVLVVLLVACPLLPQAFGGVLVLLALWLFAFDLARVTIRQRGLPRYV
                     AACLLAGCFWLGLGGALIALSTAYDAALHAVFVGFVFSMVFGHAPVILPAVLRLRFPY
                     HPVLYAPLAVLHASLAVRVLVSVPVGAWGNAAAIGLFVALAAALVLRKR"
     gene            2851..3306
                     /locus_tag="VIG70_06280"
     CDS             2851..3306
                     /locus_tag="VIG70_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250195.1"
                     /translation="MRVTLVLLLALAAGGCFAPPRTAEPLYGREAVALEPAERDFVLA
                     EMRQFVLAIRGIIEGVADNDMKGVAAAARAVGLQVHRSQIAGPGPLAAAVAHKVPAGF
                     RTLSLATHTAFDEMALHAEQVGDRDQVLASLADNLRRCVACHAAYRFPE"
     gene            complement(3319..4020)
                     /locus_tag="VIG70_06285"
     CDS             complement(3319..4020)
                     /locus_tag="VIG70_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8250196.1"
                     /translation="MRGLKGKTAIVTGGAAGIGAAVVERFKAEGARVIVFDLKGDPPI
                     DITDYAAVKKALDSAGPVDILVNNAGWDMFRPFLKTDPAFWQKIISINLVGAMNLLHC
                     VLPGMVERGRGKVVNVASDAGRVGSSGEAPYSACKGGIIALTKTLAREMASKNIQLNV
                     VCPGLTETNMLEQFMQGAGNPEKLREAYRRAVPMGRLGKPDDLPGAILFFASDDADFI
                     TGQVLSVSGGLTMHG"
     gene            complement(4070..5704)
                     /locus_tag="VIG70_06290"
     CDS             complement(4070..5704)
                     /locus_tag="VIG70_06290"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEY8250197.1"
                     /translation="MAALLPRGGIELLRDPLLNKGTAFTRAERDSLGLRGLLPPHVHT
                     QDEQAQRFLAHLHGLPSALEKYIDLNALHDRNEALFFRVVGEHPDEILPLIYTPTVGL
                     ACQQFGHIFQRPRGLFIGADDRGRIAGVLRNWPERDIGIIVLTDGERILGLGDLGANG
                     MGIPVGKLSIYTGCAGVHPKRCLPVMLDAGTDNEALRADPFYVGLKRKRLRGRAYDEL
                     VDEFVAAVQKVFPGALIQFEDFANRNAFRLLERYRDRVCTFNDDIQGTGATALAGLYS
                     ALRITGSTLPEQRILCLGAGEAATGICNTVASALVDEGLPQDDARRRCWLYDVRGLVV
                     ASRTDLGQEQRAYAHEHAYLRDFARAVNALRPTAIIGAAGVGGTFTKAVIGSMARINR
                     RPIVFALSNPTSKSECTAEQAYAWSAGRALFACGSPFDAVTLKGKTHVPRQSNNSYIF
                     PGVGLGVIASGARRVTDEMFLAAARALARQTSKADLAQGSLYPPLARIRDVSAHIAAA
                     VAGVAYEQRLTPRRRPPNLLARIRAQMYDPRYPRYA"
     gene            complement(5705..7330)
                     /locus_tag="VIG70_06295"
     CDS             complement(5705..7330)
                     /locus_tag="VIG70_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY8250198.1"
                     /translation="MRKPFAITLDPGSSLANHTGTWRTERPVYVDRLPPCNAACPAGE
                     NIQGWLAHAESGAYEAAWRKLTEDNPFPAVMGRVCYHPCETSCNRATLDSAVGINSVE
                     RFLGDEAIKRGWTFAPSSVDSGKKVLVVGAGPSGLAAAYHLARAGHKVEIHDAGPFAG
                     GMMRFGIPKYRLPRDVLDAEIRRIVELGVEIRLNTKVNSILETMKQGRFDAAFLAVGA
                     HIGKRAYIPAGTSARILDAVSVLRSMEGEGQPMLGRRVVVYGGGNTAFDVARTAKRLG
                     AAEAIVVYRRTREKMPAHDSELEEALQEGVMVKWLSTIKHASDTALTVEKMVLDEKGF
                     PQPTGEIETLEADSLVLALGQDVDLGLLDNIPQIEKQDGVVKVASNMMTGYAGIFAGG
                     DMVPSERTVTVAVGHGKKAARHIDAWLKGGVFSKKTNGETASFDKLNTWYYSDAPKTL
                     RPVLDIVRRQSTFDEVLGGLDESNALFEARRCLSCGNCFECDNCYGVCPDNAVIKLGP
                     GKRFEFNYDYCKGCGLCAAECPCGAIKMVPEEI"
     gene            complement(7330..8313)
                     /locus_tag="VIG70_06300"
     CDS             complement(7330..8313)
                     /locus_tag="VIG70_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY8250199.1"
                     /translation="MAFQPINFYQTGTFTVGNRLLAPETRSVQANMRRTNSLNSGHRA
                     CQGCGEALGARYAIDAAMNATGNQLVAANATGCLEVFSTPYPETSWQIPWIHSLFGNA
                     AAVATGIAAALKAKNKTHVRVIAQGGDGATTDIGFGCLSGMFERNDDVLYICYDNEAY
                     MNTGVQRSSATPPAARTATTSNGNDFGQGKNLPSIAMAHGIPYVATATVAELHDLEYK
                     VQRAMALRGARYIHIFVSCPLGWGHASNDSIRIARLAKETGLFPVFEAEHGEVVSVSR
                     IRRRTPVEEYLKPQRRFAHLFSPVLKADLIARIQAAADRNIRKFGLLAEAA"
     gene            complement(8324..>8732)
                     /gene="porA"
                     /locus_tag="VIG70_06305"
     CDS             complement(8324..>8732)
                     /gene="porA"
                     /locus_tag="VIG70_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEY8250200.1"
                     /translation="QGVKIGVLGVTCFRPWPHAAVRTMLGGARRVVVLEKSLAVGVGG
                     IVSTNVRTALTGRAIPVYDVIAGLGGRAITKASLARAFALAMNDQLDLTTFLDLERGV
                     VERVLAREREVRRSGPIAENILRQVGMGAAGTG"
BASE COUNT         1199 a         2940 c         3043 g         1550 t
ORIGIN      
        1 cccggcaacg tcatcttcct gcccgaccac cgcatggtga tcatcgactt cggcatggtc
       61 gggcggctct cgccgcagcg ccgcaagcag gtcaccgacc tgctcgccgg gctcgcgcac
      121 ctggaagagc gcccgatgct cgacgtgctg gtcgactggg cgggcgacgc ctacgtcgac
      181 gaggcgaagc tcgcggccga cgtgaacgag ctggtgttcg agtaccagga cgtgccgctc
      241 aaggacatcc gcatcgcgaa tgtcgtgcgc cagttctcgg ccatcgtgcg cgagcactcg
      301 atcgtgctgc ccgcggacct ttccatgatg ttcaaggcgc tcatcacgct cgagggcctc
      361 gggcgccagt acgacccgga agtgcacatc atcgatcacc tggcgccgct cttgcgcgac
      421 gcgctcgacc agcgctacca gcccgcggag ctcgtgcgcc gcgggcgcag cgcgctcgcc
      481 gagttcctca acctggtggg cagcgtgccg cgcgacttcg cccggctgct gcgcgaggcg
      541 aggcgcggca agacgcggct cgacctcgac ctcaagcgcc tcgaccagtt cggcgcccag
      601 gtggagcgca gcatcaaccg cggctcgatg gcgctcatga ccgcgtcgct cgtcatcggc
      661 tcggccatcg tgctgaacgt gccgaacggg ccgaccgtgc tcggcatcga cgtgctggcc
      721 accctcggct tcgccgggta cgcggcggcg ttcttcaaca gcctgtgggt gatctacggg
      781 gtctggcggt cggggcggga ataagcggcg ctgctcgtag aggtgccagt agggcatcat
      841 gcgcgccatg accgatcgcg accgccgcat cgtggcggcg tggctcctgg tttgcgccgc
      901 gctggtgttc gcgatcgtcc tggtgggcgg cgtgacgcgg ctcacgcgct cggggctgtc
      961 gatcgtcgaa tggcagccgc tcgtgggcgt gctgccgccg ctttcgacgg ccgactggga
     1021 agcgctcttc gcccggtacc gcgagacccc ggagttccgc caggtcaact tcgacatgac
     1081 gctcgagagc ttcaagggca tcttctggtg ggagtacgtc caccgcctgc tcgccagagg
     1141 aatcggattg gtcttctttc ttccttacct ggttttcctc atgaggaaga agctcgaccg
     1201 tcccctcgcg tggaagcttg gcggcatctt cgcgctcggc gcgctccagg gcgccctggg
     1261 atggtggatg gtgaagtccg ggctgatcga tgaccccaag gtcgacccgg tgcggctcgc
     1321 cgcgcacctc ggcctggcgc tcgcgatctt ttccgccgag ctgtggatcg ccctgcagct
     1381 cctttcgccg cgggtgcggc cgttcgaaac gccggcgctt cttctccccg gggtcgtttt
     1441 tctgatggcg ctctccggcg gcatggtggc gggcctgcgc gccgggtcgg cgtaccagac
     1501 cttcccgctg atgaacggtc atctcgtgcc gcccgaagtc ctgatgctcg agccgtggtg
     1561 gcgcaacttc ctctacaaca tggcgacggt gcagctcgtg caccgcgcgg tcttctggct
     1621 cctggcggtg ctggttccgc tcgcctggtg gcgcgcccgc ggcacgctgg caggcaatgc
     1681 gctgctcgcc gcgttcgtgc tgcaggccgt cctcgggatt tcgacgctcc tgctgggcgt
     1741 gccggtcggc cttggcgccg cccaccaggg cggcgcggtc ctgctgctcg ccgccgcctt
     1801 gtggaacgcg cactcggcgg gcgtctggcc ggcgttgcgg ccgatgcatg ccggagcgtg
     1861 aaaggatata gcctcccgct gctggtgctg ggattcgtgt cgctcgcctt cggcgtggcg
     1921 ggcgggctcg cgcgcctgtc tgcgctggaa gttcccggaa ccgcgatcgc gctgcacggc
     1981 ccgctgatgg tgtcggcgtt cttcggcacc gtgatctcgc tcgagcgcgc cgtggcgctc
     2041 ggccggccgt ggggttacgc atcgccgctg gcagcgggac tgggcggtat tttcttgctg
     2101 atcaattttc ccctgacggg ttttgcgctg atggtcctcg ccgcggcggt cttcgtcgcg
     2161 tgcggcgtgc tcgtcgtgca gcgccagccg tccctcgaga ccgcgaccct gctcgcggga
     2221 gccggcgcgt ggctcgcggg caatatgatg ctgctcgaag gccaggcggc ggtgccgtgg
     2281 tggatcgcgt tcttcgtgct cacgatcgcc ggcgagcggc tcgagctttc acgcgacctc
     2341 aagcggccgc cttgggtgcg cagagcgttt gccgtgctcg tggtcttgct ggttgcctgt
     2401 cctttgcttc cgcaggcatt tggcggggtg ctcgtgctcc tcgctctgtg gctcttcgcg
     2461 ttcgatctcg cgcgcgtcac catccggcag cgcggtctgc cgcgctatgt cgccgcgtgc
     2521 ctgctcgccg gctgcttctg gctcgggctc gggggtgcgc tgatcgcgct ttccacggcc
     2581 tacgacgcgg cgctgcatgc cgttttcgtg ggcttcgtgt tctcgatggt gttcggccac
     2641 gcgccggtga tcctgccggc ggtgctgcgg ctgcgctttc cctatcaccc ggtcctgtac
     2701 gcgccgctcg ccgtgctgca cgcctcgctc gcggtgcgcg tgctcgtctc cgtccccgtc
     2761 ggcgcctggg gcaacgccgc ggccatcggg ctgttcgtcg ccctcgcggc ggctctggtg
     2821 ctgagaaagc gctagcatgg aagcgctgcc atgcgcgtga ctctggtcct gctgcttgcg
     2881 ctcgccgcgg gcggatgctt cgcgccgccg cgcaccgccg agcccttgta cggccgcgag
     2941 gcggtggcgc tcgagcccgc ggagcgcgac ttcgtgctcg cggagatgcg ccagttcgtt
     3001 ctggcgatcc gcggaatcat cgagggcgtc gcagacaacg acatgaaagg cgtcgccgcc
     3061 gccgcgcgcg cggtgggcct gcaggtgcac cgctcgcaga tcgccggtcc cgggccgctc
     3121 gccgcagccg tggcgcacaa ggtgccggcc ggtttccgca cgctcagcct ggccacgcac
     3181 acggcgttcg acgaaatggc gctgcacgcc gagcaggtcg gcgaccgcga tcaggtgctc
     3241 gcgagcctcg cggacaacct gcgccggtgc gtcgcctgcc acgccgccta ccgatttccc
     3301 gagtagacgc cttgttcgct agccgtgcat cgtcaggccg cctgacacgc tcagcacctg
     3361 gccggtgatg aagtcggcgt cgtcgctcgc gaaaaacagg atcgcgccgg gcaggtcgtc
     3421 gggcttgccg agccgcccca tcgggaccgc gcggcgatag gcctcgcgca gcttctccgg
     3481 gttgcccgcg ccctgcatga actgctcgag catgttggtc tcggtcaggc cggggcagac
     3541 cacgttcagc tggatatttt tcgaagccat ttcccgcgcc agcgtcttgg tgagcgcgat
     3601 gatgccgccc ttgcaggccg agtagggcgc ctcgcccgag gagccgacgc ggccggcgtc
     3661 ggaggcgacg ttcaccacct tgccgcggcc gcgctcgacc atgccgggca gcacgcagtg
     3721 gagcaggttc atcgcgccga ccaggttgat cgagatgatc ttctgccaga aggcgggatc
     3781 ggtctttagg aagggcctga acatgtccca gccggcgttg ttgacgagga tgtccaccgg
     3841 gccggcgctg tcgagcgctt tcttcaccgc ggcgtagtcc gtgatatcga tcggcggatc
     3901 gcccttgaga tcgaagacga tgacgcgcgc gccttcggcc ttgaaccgct caactacggc
     3961 cgcgccgatg ccggcggcgc cgccggtgac gatcgcggtc ttgcccttca ggcctcgcat
     4021 gaaacgtctc ccgtgctcat tttccgggat tatggcggtc gtctcgggcc tacgcgtagc
     4081 gcgggtagcg cgggtcgtac atctgcgcgc ggatgcgggc gaggaggttc ggcggtctgc
     4141 gcctcggggt caggcgctgc tcgtaggcca cgccggcgac cgcggccgca atgtgcgccg
     4201 atacgtcgcg gatgcgcgcg aggggcggat aaaggctgcc ctgggcgaga tcggccttgg
     4261 aagtctgccg cgccagcgcg cgcgcggcgg cgaggaacat ctcgtcggtg acgcggcgcg
     4321 cgccgctcgc gatcacgccc agcccgacgc cgggaaagat gtaggagttg ttgctctgcc
     4381 gcggcacgtg ggtcttgccc ttcaaggtca ctgcatcgaa agggctgccg caggcgaaca
     4441 gcgcgcggcc cgccgaccag gcgtaggcct gctcggcggt gcactcggac ttggacgtcg
     4501 gattcgaaag cgcaaacacg atcggacgcc ggttgattcg cgccatcgac ccgatgaccg
     4561 ccttggtgaa ggtgccgccg acgccggcgg cgccgatgat cgcggtcggc cgcagcgcgt
     4621 tcacggcgcg ggcgaagtcc ctgagatacg cgtgctcgtg ggcgtaagct ctctgctcct
     4681 ggccgaggtc ggtacggctg gcgacgacca ggccgcgcac gtcgtagagc cagcagcgcc
     4741 gccgggcgtc atcctgcggc aggccctcgt cgaccagcgc cgacgccact gtgttgcaga
     4801 tgccggtcgc cgcctctccg gcgccgaggc aaagaatgcg ctgctccggc aacgtcgatc
     4861 ccgtgatgcg cagcgcgctg tagagcccgg cgagcgccgt ggcgccggtg ccctggatgt
     4921 cgtcgttgaa ggtgcagacc cggtcgcggt agcgctccag cagcctgaag gcgttgcggt
     4981 tggcgaagtc ctcgaactgg atgagcgcgc cgggaaacac tttctgcacc gcggccacga
     5041 actcgtcgac cagctcgtcg tacgcccggc cgcgcagccg cttgcgcttc aggccgacgt
     5101 agaacgggtc tgcgcgcaat gcctcgttgt cggtgccggc gtcgagcatc accggcaggc
     5161 aacgcttcgg atgcacgccg gcgcagccgg tatagatcga gagcttgccc accgggatgc
     5221 ccatgccgtt cgcgccgagg tcgccgagcc cgaggatgcg ctccccgtcg gtgagcacga
     5281 tgatgccgat gtcgcgctcg ggccagttgc gcagcacgcc ggcgatgcgg ccgcggtcgt
     5341 ccgccccgat gaacaggccg cgcggcctct gaaagatgtg gccgaactgc tggcaggcga
     5401 gccccacggt gggggtgtag atgaggggca ggatctcgtc cgggtgctcg cccaccacgc
     5461 gaaagaacag cgcctcgttg cggtcgtgca aggcgttgag atcgatgtac ttctcgagcg
     5521 cgctgggcag gccgtgcagg tgcgcgagga agcgctgcgc ctgctcgtcc tgggtatgga
     5581 cgtgaggcgg cagcaggccg cgcagtccga gcgagtcgcg ctccgcgcgg gtgaacgcgg
     5641 ttcccttgtt gagcagcggg tcgcgcagca gctcgatgcc cccgcgcggc aacagcgccg
     5701 ccattcagat ctcctcggga accatcttga tcgcgccgca ggggcattcc gcggcacaca
     5761 gcccgcagcc cttgcagtag tcgtagttga actcgaagcg cttgccgggc ccgagcttga
     5821 tcaccgcgtt gtcggggcag acgccgtagc agttgtcgca ttcgaagcag ttgccgcacg
     5881 acaggcagcg gcgcgcttcg aacagcgcgt tgctctcgtc cagcccgccc agcacctcgt
     5941 cgaatgtgga ctggcggcgc acgatgtcga gcaccggccg cagcgtcttc ggcgcgtcgg
     6001 agtagtacca ggtattgagc ttgtcgaagc tcgccgtttc gccgttcgtc ttctttgaaa
     6061 agactccgcc tttcagccac gcgtcgatat gccgcgccgc cttcttgccg tgaccgaccg
     6121 ccaccgtgac ggtgcgctcg gaaggcacca tgtcgccgcc ggcgaagatg cccgcgtacc
     6181 cggtcatcat gttggaggcg accttgacca cgccatcttg cttttcgatt tgagggatat
     6241 tgtccaaaag gccgagatcg acgtcctggc cgagcgcgag cacgagcgag tcggcttcca
     6301 gcgtctcgat ctccccggtg ggctgcggga agcccttttc gtcgaggacc atcttctcga
     6361 ccgtgagcgc cgtgtcggat gcgtgcttga tggtggagag ccacttgacc attacgccct
     6421 cctgcagcgc ttcttcgagc tcggagtcgt gcgcgggcat cttctcgcgc gtgcgcctat
     6481 agacgacgat ggcctcggca gcgccgaggc gcttcgcggt gcgggcgacg tcgaacgcgg
     6541 tgttgccgcc gccgtagacc accactcgcc ggccgagcat cggctggccc tcgccttcca
     6601 tcgagcgtag caccgacacc gcgtcgagaa tgcgcgccga agtgcctgcc gggatatagg
     6661 ctcgcttgcc gatgtgcgcg ccgaccgcga ggaacgcggc gtcgaaccgg ccttgcttca
     6721 tcgtttccag gatgctgttg accttggtgt tcagccggat ctcgaccccc agctcgacaa
     6781 ttctccggat ctccgcgtcg agcacgtcgc gcggcaggcg atacttgggg attccgaagc
     6841 gcatcatgcc gccggcaaac ggcccggcat cgtggatctc gaccttgtgc cccgcgcgag
     6901 cgaggtggta ggcggcggcg aggccggagg gcccggcgcc caccacgagg actttctttc
     6961 cggaatcgac ggatgaaggg gcgaacgtcc agccgcgctt gatcgcctcg tcgccgagga
     7021 agcgttcgac cgagttgatg ccgaccgcgc tgtcgagcgt cgcccggttg caggaagtct
     7081 cgcacgggtg gtagcacacg cgtcccatga cggcggggaa ggggttgtct tccgtcagct
     7141 tgcgccacgc cgcctcgtag gcgccggact ctgcgtgcgc aagccagccc tggatgttct
     7201 cgccggcggg gcaggcggca ttgcagggcg gcagccggtc gacgtagacc ggccgctcgg
     7261 tgcgccaggt gccggtatgg ttggcgaggc tcgagcccgg gtcgagggtg atggcgaagg
     7321 gcttgcgcat catgcagcct ccgcgagcag gccgaacttg cggatgttgc ggtcggccgc
     7381 ggcctggatc cgggcaatga ggtccgcctt caggacaggg gaaaacaggt gcgcgaaacg
     7441 ccgctgcggc ttcaggtatt cctcgaccgg agttcgtctg cggatcctgg agacgctcac
     7501 gacctcgccg tgctccgctt cgaacaccgg gaagagtccc gtctccttcg ccaggcgcgc
     7561 gatccggatc gagtcgttcg aggcgtggcc ccagccgagg ggacacgaca cgaagatgtg
     7621 gatgtagcgg gcgccccgga gcgccatcgc gcgttgcacc ttgtattcga ggtcgtgcag
     7681 ctccgcgacc gtggccgtgg cgacgtaggg aatgccgtgc gccatcgcga tcgacggcag
     7741 gttcttcccc tgcccgaaat catttccgtt gctggtggtg gcggtgcgcg cagccggagg
     7801 cgtggccgac gagcgctgca cgccggtgtt catgtaggcc tcgttgtcgt agcagatgta
     7861 gagcacgtcg tcgttgcgct cgaacatccc cgacaggcag ccgaagccga tgtcggtcgt
     7921 ggcgccgtcg ccgccctggg cgatcacgcg gacatgggtc ttgttttttg ccttaagagc
     7981 ggcagcaatc ccggtcgcga ccgccgcagc gttgccgaac agcgaatgga tccacgggat
     8041 ctgccacgag gtctcgggat agggcgtcga aaacacctcg aggcagccgg tcgcattggc
     8101 ggcgaccagc tggttgccgg tcgcattcat cgccgcgtcg atcgcatagc gcgcgccgag
     8161 cgcttccccg cagccctggc aggcgcggtg gcccgagttc agcgagttgg tgcggcgcat
     8221 gttcgcctgc accgagcggg tctccggcgc aagcagccgg ttcccgacgg tgaacgtgcc
     8281 ggtctgatag aagttgatcg gttggaacgc catcggctgc ctcctacccg gtacccgccg
     8341 cgcccatacc gacctgccga aggatgttct cggcgatcgg gccgctgcgg cggacttcgc
     8401 gctcgcgcgc gagcacccgc tccacgaccc cgcgttcgag atcgaggaag gtcgtgagat
     8461 cgagctgatc gttcatcgca agggcaaacg cacgggcgag cgaggccttg gtgatggcgc
     8521 ggccgcccaa gccggcgatg acgtcgtaca ccgggatggc gcgcccagtg agcgcggtgc
     8581 gcacgttggt agaaacgatg ccgccgacgc ccaccgcgag gctcttctcc agcactacga
     8641 cgcgccgcgc gccgccgagc atcgtgcgca cggccgcgtg cggccacggc cggaagcagg
     8701 tcacgccgag aacgccgatc ttcacgccct gg
//
LOCUS       DATBSZ010000098        37557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2336959, whole genome shotgun sequence.
ACCESSION   DATBSZ010000098 DATBSZ010000000
VERSION     DATBSZ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 37557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37557
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2336959"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VIG70_06310"
     CDS             <1..820
                     /locus_tag="VIG70_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8250201.1"
                     /translation="TDKIVWENGNRYTFRLRPSTYMQTAMLIPEAVKLKKKRWAIVYP
                     NYEYGQSATASFKRLLKAAQPDVEFVAEQAPPLGKIDAGAVVQALADAKPDAIFSSLF
                     AADLQRFVREGNTRGLFRNTPVFNLLAGEPEYLDTLKDETPDGWWVTGYPWSEINTPE
                     HRAFREAYQKRWKDYPRQGSVVGYTAVYAVAEAFRKAKSVETEKVVEALKGLKMQTPF
                     GPIEWRALDHQSTMGAYVGQLARKGGKGEMVNWRYADGTKVQPSDGEIRALRKE"
     gene            831..2651
                     /locus_tag="VIG70_06315"
     CDS             831..2651
                     /locus_tag="VIG70_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8250202.1"
                     /translation="MSSSAVLLQFLNGLAGASSLFLVAAGLSLIFGVSRIVNFAHGSL
                     YMLGMYLAVWLSARVGFWASVPLAAGVVAAVGLVIEVLLLRRIYRAPELLQLLATFAL
                     VLVIKDFALWAWGPEDMLGPRAPGLASAVTIAGRQFPQYDLFLIVLGPAVFVALHVLL
                     RHTRFGILIRAATEDREMAGALGVNQRLLFTMVFALGAALAGLGGALAIPREPANLEI
                     DLALVSDAFVVVVVGGMGSIPGAFLAALLIGEIKAFCIGLGYSEVTLVAEFIVMAVVL
                     VLRPWGLLGRPLAEARGLTPTGDFDVASRNPLLIALVALAVVPLLSDGYALVLLTDVL
                     VFALFAVSLHLLLGPGGMVSFGHAAYFGLGAYAAALLFLRWRLPMELALLAAPLAAAA
                     GAALFGWFCVRLSGIYLAMLTLAFAQIAWSVAFQWDALTGGSNGLVGIWPAPWLASRT
                     AYYFVALVLCAGAIACLWRISASPFGYALRGSRDSPLRAGAIGIDVRTQQWAAFIVAG
                     SFAGLAGALFAFSKGSISPETLSVPRSVDALVMVLLGGVQTLTGPVWGAALFTWLEDA
                     VSREVAYWRAAIGAVILLLVLAFPQGIGGFFGQALTRWSR"
     gene            2639..3379
                     /locus_tag="VIG70_06320"
     CDS             2639..3379
                     /locus_tag="VIG70_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8250203.1"
                     /translation="MVALTVNRISKAYGGVQAVREVSFSVAPGEMVAIIGPNGAGKTT
                     CFNMLNGQLVPDSGEIRLGDASLAGLPPHRVWRLGVGRTFQVTATFGSMSVRENVQTA
                     LRSHHKKNYSLWSRARGSFGQEAEALLERVGMRSQAERPCAVLAYGDLKRVELAIALA
                     NRPRLLLMDEPTAGMAPEERGALMALAATLARDDRLAVLFTEHDMDVVFGFASRVIVM
                     HLGEVIAAGTPAQVRADARAREVYLGDA"
     gene            3342..4016
                     /locus_tag="VIG70_06325"
     CDS             3342..4016
                     /locus_tag="VIG70_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8250204.1"
                     /translation="MRVHARSTLATLDIRGLSAGYGRAQVLFDVALEARQGEVVALLG
                     RNGAGKSTTLKAIMGLIEPLSGEVIFEGRNLKDLEPHEIARLGLGYVPEDRRIFTDLT
                     VTENLEVARQPPRGAAPAWTEERLFALFPALAGLRNRRGARMSGGEQQMLCIARTLAG
                     NPRAILLDEPSEGLAPLVVEQVARAIVELKRSGITVLLAEQSRHFAGRVADRAYVLEK
                     GIIRGA"
     gene            4013..4477
                     /locus_tag="VIG70_06330"
     CDS             4013..4477
                     /locus_tag="VIG70_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250205.1"
                     /translation="MKRGLLLTLTEPPPGMEEEFNAWYDEEHLPERLAIPGFRSARRW
                     VAGRTYLATYELDSPEVLSSAPYLARYRNQTPWSRRCLGKCVTFKRWACEQLDPGDAD
                     PHPGAKGLILAMDARSPPRVVQARRFAADSGERITLYELSDPDPTGYRLYKS"
     gene            4452..5360
                     /locus_tag="VIG70_06335"
     CDS             4452..5360
                     /locus_tag="VIG70_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY8250206.1"
                     /translation="MATASTSLERYAWLSIAAALATIGLKTAAWWLTGSVGLLSDALE
                     SLVNLAAAMLALSMLRLAASPPDEDHPYGFSKAEYFAAGIEGALIVLAAGGILAAAIP
                     RLIEPRALDAPILGLALTVAASAINFAVAVVLVRVGRREHSITLEADGQHLMTDVWTS
                     AGVIIGVAAVLATGWLRLDPLVALAVAAHIVWTGLRLMRRSWKGLLDAAISTEDTGEV
                     TKLFKEYSRRQGVAFHALRTRQAGARRFISFHLLVPDAWSVARAHQLSEEIESRIRSM
                     VPNAAVFTHIEPISDPASYDDQGLDR"
     gene            complement(5373..6968)
                     /locus_tag="VIG70_06340"
     CDS             complement(5373..6968)
                     /locus_tag="VIG70_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEY8250207.1"
                     /translation="MWSSLKPGPANYTALSPIVFLPRAAEIFPDRVAVLHGSTRFTYK
                     QLFERAKRLASALQKAGVKRGDVVSAMLPNIPAMLEAHYGVPMLGAVLNTINTRLDAD
                     TVAYILEHGEAKVFITDRMFAGVCGPALAKLKKKPRVVDVDDPQYGGPGERLGQVEYE
                     AFLKTGSPGFEWSLPQSESDPIALNYTSGTTGRPKGVVYHHRGTFLEACGNVMAWAMP
                     ASPVYLWTLPMFHCNGWCFPWSVTAMAGTHVCLRAVEPKRIFELIVEHGVTHMCGAPT
                     VLGMLIAAPEGERRKFPHPVHIQTGGSPPPAKVIQAMEELGFQVLHIYGMTELQGPST
                     FCAPQDWDDKYTEMARQGVRYPVVEGHIVADPKTCKPLPKDGSAIGEILVRGNTVMLG
                     YLKDAKATAEAFAGGWMHTGDLAVWHPSHYVEIKDRKKDIIISGGENISSVEVEIALY
                     KHPATQLAAVVARPDQKWGETPCAFVQLKPGASATEQDYIDWCRQNLARFKIPKKVVF
                     GPVPTTATGKIQKFVLRERAKAL"
     gene            7078..8253
                     /locus_tag="VIG70_06345"
     CDS             7078..8253
                     /locus_tag="VIG70_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8250208.1"
                     /translation="MKTLAIAVALALSTSAFAQEKSIRIAGFGAKSGVVRSFGINSEA
                     AMLAAADVINKQGGVKLADGSKAKLVIDYLDDRCTAEEGISVLRRIYGTDAIVAVGPT
                     CSNVAEPLFGILQKKVDDKGDSGLQMPVFTDVAIKIGLAKISEWAFRNVPNESIMYSS
                     LFYWLKTKYPNAKTVFGGVEEDFAHSRASWYAIMKDRAEANGYQVLGNSKWLLNDTNF
                     ATQVREMKNANPDIVAISAHPFTTCGVLKEMQRQGVKPKVLVGLTSSSSLETLQGCAK
                     QAEGMIIPTSFAPVTKEGKTAAETTAKFKGAADLHSMAAWENMFILKQVIESEKISGD
                     KSQLQADRRKIRDGLAKLRETKGLMGVTKRTDDREADKPYLFVQANKGEWKVLHNPL"
     gene            8260..9123
                     /locus_tag="VIG70_06350"
     CDS             8260..9123
                     /locus_tag="VIG70_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8250209.1"
                     /translation="MLDFLQAVCDGLLFGATYALIGIGFTLVFGVMHKLNLAYAAASV
                     GAAYAGLLILQLAAGAPPWVVFLCAFLGGGVIGWLVYVTCFHFIPLSNQLAPLMATVG
                     MLLLIDEIIVHLTAGMPQNYPAMFDDVMLRAGEFTLRGDLLLVAGVSVVCMIALLALL
                     YRTRLGLATRAVSQQAVAAQLCGIRVGQVNAMTFIVTGLLGGIAGALIGASVGALSPL
                     LTAPLTVKGLIVTVIGGLGSIPGAIVAGLLVGAFENVFQQFRGVTERDLYVMLLLFAF
                     LIFRPSGLFKQ"
     gene            9123..9986
                     /locus_tag="VIG70_06355"
     CDS             9123..9986
                     /locus_tag="VIG70_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8250210.1"
                     /translation="MDFYYGLAQTVGIHTLLGLSAYIVLLTGQVTLAQAAFFAIGAYA
                     AGMLTVLAGWHIVPALGASAMFGAATACAVGFPALRVKGLMLVVATLAFGEGVRLFFF
                     NFDYQVAKGEIKVGPLGGEGFRQIRYFQEHGWSTGEVMLFVWVFVVLAMAALWWLDRS
                     RYGAVLRAVGEDELAAQSVGVNLTAVKVSAMTVGGAVAGIGGGLYAHYTTHIEHANFN
                     VLLATFAIAYPIVGGLKNVFGTLAAVIFVQAFLIEGLRFLGDWRNLLFGALIVAAMNL
                     RPQGFLGGRRA"
     gene            9979..10722
                     /locus_tag="VIG70_06360"
     CDS             9979..10722
                     /locus_tag="VIG70_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8250211.1"
                     /translation="MLELKGLSRHFGGVKAVDGLDLAVRQGEIVGLIGPNGSGKSTTV
                     NVIAGLFPVTAGEVRFNNESLEGLPTHKRLQRGIARTFQNIRLFGQLSVWQNLWVAQN
                     APEDHARRGFFARWLGGQSGVRAEIAQLLEFSGLAHKQDELAANLAFGEQRRLELARA
                     LAAKPKLLLLDEPAAGMNSEEIGQLRDRILKLRAQGITILLIEHVMELVMGVTDRIAV
                     LNFGQKIAEGSPAEIQADPLVRKAYLGGA"
     gene            10722..11426
                     /locus_tag="VIG70_06365"
     CDS             10722..11426
                     /locus_tag="VIG70_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004200384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8250212.1"
                     /translation="MLRVQDLVTAYGKIEALKGVSLEVAQGSITCLLGPNGAGKTTLM
                     MTLAGILRPRSGSIRFSEDEITGLPPHAIVARGIALVPENRLVFPSMTVLENLQAGAY
                     QRRNGFEDDIERMFTRFPRLKEREAQPAGTLSGGEQQMLAVARALMSRPKLLLMDEPS
                     LGLAPLVVEEIFAIITELHRDGATIFLVEQNAHMALRVAQRFYLMEQGRVTFSGDPGS
                     LAEDEVIRRAYLGSSR"
     gene            11410..12288
                     /locus_tag="VIG70_06370"
     CDS             11410..12288
                     /locus_tag="VIG70_06370"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8250213.1"
                     /translation="MAPVVDFLQNSLDGLSAGAAYALLAVGFTLIFGVLRRANLSYGP
                     AIMLGAYAATWLFLQTGAGLLVLFAVAVLATVLAGSYVERLCFAPHRRGAATAAMVAS
                     FAIWMQLEELATLLLPRHLYAFPALYEGGPLTFGPFFVRVESLLILLSAVAVCVAGWK
                     VLYATRFGIAVRAVIDDPRAAELSGIDVGAVSLRIFAVASAIGGATGFLIVASHEQVT
                     PMLGMWSTLKGMLAMMLGGLGSLPGAIAGGLALGLIEAHSQWYFGPQFRDFIAYLLLF
                     ALLALRPPWMNTRSAY"
     gene            12261..13145
                     /locus_tag="VIG70_06375"
     CDS             12261..13145
                     /locus_tag="VIG70_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8250214.1"
                     /translation="MDEYAIGVLTSVGLLSFVATSAYLLLIAGELSFGQQAFFGVGAY
                     AAGIATAMLGLPLFLGLLIAMVCGAAVAALVGLPALRLRGLYFAMATLAAAEMLRILF
                     ELFHWQVEVGGELVGPDGTQGFRGIRWVFERGVEPLGFLALVWGMLAVVLVVIHLLER
                     TRLGSAVRALGEDPELAGTLGIDVLRLKLAVVAAAGALAALGGALFAHHNTYIEPRNF
                     DIMLGVHTLAYALIGGLGTVVGPLLGVALDLGLLEGTRVFQGYRMIVFGGLVALLLIW
                     RPRGLLDERVVHWLRTRR"
     gene            13142..13537
                     /locus_tag="VIG70_06380"
     CDS             13142..13537
                     /locus_tag="VIG70_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEY8250215.1"
                     /translation="MRWLLGLTMVLSFEAVAQKAEARLDCIHTGKDFIYDCVIEIQRQ
                     GLPLTDLDVRMSADMPSMPMAHQVKPVRADATSTPGEYRARLDLEMGGEWAVKLRLAG
                     PVRDQLVLHYEFDERGARPVRGSGKPPRK"
     gene            complement(13506..14084)
                     /locus_tag="VIG70_06385"
     CDS             complement(13506..14084)
                     /locus_tag="VIG70_06385"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEY8250216.1"
                     /translation="MADEKMLDVILREARSHGNFHDRPVDQALLRKAHELMKWGPTTA
                     NSQPARIFYLFSKESREKLRPALSAVNVEKTMKAPLVALVAYDTRFYEHLPRLYHNPD
                     AINWYNKPAVAEPNAFRNSSLQGAYLIIALRAVGLDCGAMSGFNNATLDAAFFPDGRL
                     KSNFLINIGYGTGQGLPPRNPRLTFEEACRIL"
     gene            14095..15243
                     /locus_tag="VIG70_06390"
     CDS             14095..15243
                     /locus_tag="VIG70_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8250217.1"
                     /translation="MVELSSVIRPGDGILWGQACAEPQTLVEALVSQRGRLGGVGCFL
                     GSSYSGIVKAEHADDLTLSSYCGIGTNRALADAGVLGILPVPYSQLGSLIRSRAIPAD
                     VVLVQVSPPNARGEYSLGLAADYLVPALDTCRAIVAEVNEQVPWTHTERLLTRADFDL
                     VVESSRPPATPAARPPGDLEQAIARNAAAFVPDGAVLEFGIGALPDAVCSALGRHEGL
                     RVHSGSIGDGVIELAERRIVSRIDCAMLIGSRKLFDFARDNDRVRLRSSEYTHNAEVL
                     ARQPRFVAINSAVEVDLTGQVSGELAGASYVGAVGGALDFVRAANQSAGGIAITVIPS
                     SRIVERISSTVSVPRSEAGVIVTEKGAADLRGCGLRERERRMRAISGR"
     gene            complement(15288..16547)
                     /locus_tag="VIG70_06395"
     CDS             complement(15288..16547)
                     /locus_tag="VIG70_06395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8250218.1"
                     /translation="MPATCATSGRSSASNLPKRLSTPLAVLAACFVLNLFGRGLGDTY
                     AVFLLPLEREFGWTRSELTGVYSVYLLVNGLTAPLVGLVFDRLGPRWVYGAGMASLGV
                     AFFLAGGLVDLWQFYLLIGALVGIGVSLNGMVPASALLARWYRERLSSAIGIAYSAFG
                     AGALIFIPLAQYLVGELDWRATYRLMGGALLLLAPLIVFGLPWKTYAAGHPAARAEAR
                     GKAGAGWTLAAALRAPLFWGLVQVFFCTSIAMFSIIVQLVVFLVDAGFSPLAAAGAFG
                     VVGLLSAASVMGSGFASDRFGYRQTISLSFAGTASGMALLLAMTAAPSALLLALFVPL
                     FGLCMGVRGPIVSSVAARYFAGPRVATIYGLIYASNAVGAAIGAFVGGLLHDLTGGYR
                     VGLVFALVFVALAAAPFWTIPALRNFR"
     gene            complement(16486..17670)
                     /gene="pncB"
                     /locus_tag="VIG70_06400"
     CDS             complement(16486..17670)
                     /gene="pncB"
                     /locus_tag="VIG70_06400"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY8250219.1"
                     /translation="MIIQSLLDTDLYKFTMMQVVLHHFPGAQVEYKFKCRTEGVDLRP
                     DLPAIEAELVKLCSLRFRDDELTYLRGLRFMKSDFVDFLALFHFSERYVRLGAGEKPG
                     EIDITVRGPWLHTIMYEIPLLAIVSELYFRRTQPKPNLAEGRRRLKAKIGVARAVEPE
                     LGFKISDYGTRRRFSRDWQEEVLKTFKRDIPQNFAGTSNVWFAMRNNLTPLGTMAHEY
                     MQACQALGPRLRDSQTFAFDKWAQEYRGDLGIAVADTYGTDAFLRDFDMYFCKLFDGA
                     RHDSGDPFAWGERVIAHYRRNRVDPRTKTLIFSDQLTVPIAIEIARRFHGRALTAFGI
                     GTNLTNDLGHEPINIVIKMTECNGQPVAKVSDAPGKTISKDPGYLRYLRQVFGLEPAE
                     KT"
     gene            17697..18278
                     /locus_tag="VIG70_06405"
     CDS             17697..18278
                     /locus_tag="VIG70_06405"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY8250220.1"
                     /translation="MCGRYALHANPDVVALQFGLESPPEFLPRYNIAPATDILVVRNH
                     EAALARWGLRGRFVNLRAETVASKFKASARCLVPVSGFYEWKREGGRKQPFYFFPENE
                     PLFGFAALWERDTCSLITTEPNAVIGKVHDRMPLIVPREGYAAWLAGDESILATAPEI
                     AMLAHPVSMAVNQAANDSPKLIEPVELAPGLFD"
     gene            complement(18255..19295)
                     /gene="dinB"
                     /locus_tag="VIG70_06410"
     CDS             complement(18255..19295)
                     /gene="dinB"
                     /locus_tag="VIG70_06410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY8250221.1"
                     /translation="MRRIVHLDMDAFYAAVEQRDDPDLRGKPVAVGGRPESRGVVAAA
                     SYEARVFGVRSAMPMARAVRLCPELVVVPPDFERYRGVSVQVMEILRACTPLVEPLSL
                     DEAYLDVTENLWGEPLGSRVARRLKNEILERTGLTASAGVAPNKFLAKIASGYRKPDG
                     LTVISPERVESFLQELPVEALWGVGPVTARKLKEIGLEKLVQVRTVDMELLRRTVGSL
                     ADWLRQLSHGDDPRPVEPNRESKSSSTENTYAQDLTELEAIRAELDRMARENAEWLAR
                     NHLTARTITIKVRYADFKTVTRSHTLSMPTDDADVIASWAVELMARTEAGRRPVRLLG
                     VRVQGLVEQPGG"
     gene            19317..19946
                     /gene="lexA"
                     /locus_tag="VIG70_06415"
     CDS             19317..19946
                     /gene="lexA"
                     /locus_tag="VIG70_06415"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY8250222.1"
                     /translation="MDELTERQAEILRLVRELTEVSGYPPTRAEIAQRMGFRSVNAAE
                     QHLRALEKKGAIEISSGASRGIRVRDGRSAGRLGRLLELPVVGRVAAGSPILAEQHLD
                     GRYQVDPNLFTPRADYLLRVRGMSMRDAGILEGDLLAVHRTHEARTGQIVVARLADEV
                     TVKRYRRRGHAVQLEAENPEFAPIEVDLRHDALTIEGVAVGVIRNGKTF"
     gene            20031..21839
                     /locus_tag="VIG70_06420"
     CDS             20031..21839
                     /locus_tag="VIG70_06420"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY8250223.1"
                     /translation="MNHSTTANGTNTGLEVSTHAVEAEIRDRADPQQVLADRVAQLYR
                     QMPIAIGATFIAGLIATFELQGRWLKELVLIWWGLVVAYSVAASLLLYAYHRSADKVT
                     TAPEWLRWLGIAALGNGASWGLAGGVFFRSLSDEQQVFLAFLFAGMASVGIPVYAASW
                     PIFALYAAGILGPFFYVLLTFGNRLFVEIALLVPLFYVINVAIAYRLTHVFHSGYRLR
                     HAYGKLAQDHRLLNQRLERQLVELEEARRQVEASGRKLALFAERAPIAVLELQPDGTV
                     AEVNHAAEILFGYAAAELVGGPVKKLVLAEFHDEFDRIWLQLLSSRESIAGLKIRNPR
                     RDGIELTCEWTVTPLVNFQKDVIAVIAQGRDVTAQLEAERMKKEFTSTLSHELRTPLT
                     SIIGSLQLINSGVMGDVDKEVGELTMVAERNGQRLLDLINDILDIEKIESGKVTLAPE
                     VMRVDELVRESLVLNKAFGERFKVSFQSDGDIPERQVHADRKRLLQVMTNLLSNAAKF
                     SPEGGIVEISTEDAGPNVRIAVHDRGSGIPEAFRSRIFGRFTQADSTATRQKGGSGLG
                     LAICKRLIELMRGRIGFDDRPGGGTTFWFELPRRTA"
     gene            21850..22239
                     /locus_tag="VIG70_06425"
     CDS             21850..22239
                     /locus_tag="VIG70_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8250224.1"
                     /translation="MPARPLNRICYVEDDEDIQRIVRMSLERVGKMTVAVVGDPTQAI
                     AMITEFNPDLVMLDWMMPVMDGPTLFRQLKLRPETSALPVVFITARASQPDLDELKTL
                     GAAGTISKPFSPKDLPDQLRAIWAALP"
     gene            complement(22236..23501)
                     /locus_tag="VIG70_06430"
     CDS             complement(22236..23501)
                     /locus_tag="VIG70_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY8250225.1"
                     /translation="MRCLIIDDDEDLRNLLSRYILGQWPDAEIEAYDPLERDLPDASF
                     ALGSYNVIVLDYMLGRGDGLEWLKVLKQRTDCPKILFLTGAGNEIIAVRAMKAGADDY
                     QRKQELTREKLIASLRELTASAVERTVSPDLAARMEAHSLGAKVRIPGIEVLHLIGEG
                     GTARVYLASRENDDEPLVVKILRQEIVLNKNALERFMEEYAMVERIQSRHVARIFGHG
                     NDQNNVYLVMEFFEGGDLSKRLGGKPVPPLEALKFFRELMFALGDIHEKGILHRDLKP
                     QNLMFRRDGSLAIVDFGIAKNIAAADRTGAGEVLGTPRYMSPEQVQGRALDLRTDIYS
                     AGVLLYQMLTGKHLFDGETAMEVAMHHLNTQPPPLPDHLEQYQRLIEKLVEKDREARF
                     RNADEVIGFLARKLEQLPAEPGDKTQVLH"
     gene            complement(23522..23827)
                     /locus_tag="VIG70_06435"
     CDS             complement(23522..23827)
                     /locus_tag="VIG70_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY8250226.1"
                     /translation="MNAEDYKVTVAKDLADLIPVFMKNRHKELDTLRTALAAANFEQL
                     RQLGHRMKGVGNSYGFTHVSTLGKYIEDGARSGDRAALQASIDEYQDYLAKVQITYE"
     gene            complement(23859..24347)
                     /locus_tag="VIG70_06440"
     CDS             complement(23859..24347)
                     /locus_tag="VIG70_06440"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8250227.1"
                     /translation="MVEILRLISSYRTRRPARVSAFLDALFTRLARCARAEAGATEDR
                     IWDVWMHHPHHAAARELDLATGDIVARRYDLAETRLTRLLRRAPDFAEAWHKRAALYY
                     MLGRDRECLDDIRRTLELEPRHFGAMLHFAEILRSADAVAEASFAMLAARSIHPHLTI
                     PD"
     gene            complement(24358..27330)
                     /locus_tag="VIG70_06445"
     CDS             complement(24358..27330)
                     /locus_tag="VIG70_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEY8250228.1"
                     /translation="MAFLPDYAELHCLSNFTFLRGASHPGELVARAHALGYKALALTD
                     ECSLAGAVRAHQEAKECGLDLILGTEILVEESLKLVALATDRRSYGAISSLITIGRRR
                     SKKGRYSITREDLEGLAGSGALLLWIPGEDFTSAAWLAERFDGAAWIAAELHCGPNDC
                     AKMGSLKEISRKSGLPLVAAGDVHMHVRSRRRLQDVLTAVRLGKPVAQCGRALFPNAE
                     RHLRLRVRLAQLYPPELLAETVAIAERCRFSLDELRYEYPQELVPENETPASWLRRLT
                     EEGLRRRFPAGVPPKVAAQVEHELRLIAELGYEPFFLTVHDVVRFARGEGILCQGRGS
                     AANSVVCYALGITEVDPDRMNLLFERFVSRERNEPPDIDVDFEHQRREEVIQYVYGKY
                     GRDRAALAATVICYRTKSALRDAGKALGLPLDEVDRLAKSFTFWDQKLQPSSPLFELA
                     GALRGFPRHLSQHVGGFVVSRGPLAELVPIENAAMPERSVIQWDKDDLEALGLLKVDV
                     LALGMLSAIRRALSMIGIEMQDVPAEDPEVYRMIQKADTIGVFQIESRAQMSMLPRLR
                     PASFYDLVIEVAIVRPGPIQGGMVHPYLRRRRGLEPVVYPSEAVKRVLSRTLGVPIFQ
                     EQVMELAMVAAGFTPGEADRLRRSMAAWKRSGGLEPFEERLKSGMQRNGYPAEFAEAI
                     YRQILGFGEYGFPESHSASFALLVYVSSWIKCHYPAAFCAALLNSQPMGFYAPAQLVQ
                     DARRHGVEVRPPDVQASDWDCTLEGNALRLGLSMVSGLGEKEGRRIAAGKPYGRVLDL
                     KLSKRDLRHLAAAGALQSLAGHRRLAHWAAAGAARRAPLDAPAPEPLPRLEAPREGEE
                     IVADYASLGLTLGRHPLALLRKRLDAMRLLRAEELKSRPHGSPAQVAGLVTCRQRPDT
                     ASGVVFVTLEDETGCMNIVVWRNLVESQRRELLGARLMGVQGVVERDGDVVHLVARHL
                     SDYSALLGPLAALSRDFH"
     gene            complement(27314..28486)
                     /locus_tag="VIG70_06450"
     CDS             complement(27314..28486)
                     /locus_tag="VIG70_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEY8250229.1"
                     /translation="MLWVALHFPRLPTQALGPIAAWACQFTPKVSLEPPRELLLEVQG
                     SLRLFGGFEPLQQKLVAGLAELGFAFSLAQALTPRAALWLARGEGKTLEALPVEVAGL
                     DAEFFRSIGISTIGALLELPREGLGQRCGARVLEELDRALGAAPEPRAFFAPPPHFDA
                     SLELPAEVAQAEGLLFGARRLLAHLEGLLAARHEGIRSFLLKLIHPQGGCTEVQVGLA
                     STTRHAERLAQVLRERLARLSLMEPVASIRLEARDFAPLPGTSRGLFGDPASEAEGWA
                     RLVERLRARLGHGAVCGLAAYPDHRPEHAWRRVEPGEWDPRESPVPGPRPLWLLEPKK
                     LNEGEFTALAGPERIESGWWDGDDAKRDYFVARLPNGALAWIYREAQAWYLHGLFA"
     gene            complement(28401..29105)
                     /gene="imuA"
                     /locus_tag="VIG70_06455"
     CDS             complement(28401..29105)
                     /gene="imuA"
                     /locus_tag="VIG70_06455"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HEY8250230.1"
                     /translation="MAALEEILQRHPVWLGESKAAFSPAVPTGYPSLDQALPGGGWPA
                     AALTEILPTQAGIGELQLVLPSLAALSWAGKRVVWLAPPHLPYAPALAAAGVDLAHLA
                     VVRAPGRRDALWAAEQVLRAGSCHALIGWFRRAGYDELRRLAVAAEGSAAWVALFRPR
                     EAARDSSPAALRIALDPDGDALAVRILKRRGAPAAASFSLQVKRPFHALGRASFPAPA
                     DASARADRRLGLPVHA"
     gene            complement(29133..30272)
                     /gene="tagH"
                     /locus_tag="VIG70_06460"
     CDS             complement(29133..30272)
                     /gene="tagH"
                     /locus_tag="VIG70_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HEY8250231.1"
                     /translation="MITVKITKRAADGRPTLYATRDLDVLPVSIGRDAACTIALEDPN
                     KHMSRFHVEIEEEGGTYWMSVVSKVNPVMVKGRRYGPGTRLTLQSGDSFELGDYEVHV
                     LLPESGPAAQAEPATQPPAARPAAIPADQAERLFNESTFLGGDQPIAAKKPMAQPAPM
                     PEETFIPQKKPAAAPPAPGAGPSDALRAFLEGAGLPPKELPPREADRMLRDCGAILRA
                     AIDGIMMLLLARAEMRKEFDTGERTMVAARDNNPLRMMSDPREAMDFLFDPGERTDGF
                     LDPVQAVRDACEDLRSHERALMAGMRAAVLGALRRFDPPALERAFEKNAKGFSLSSRK
                     AKLWEAFVAGQEKLSRDAQEDFNKAFGRDFMGAYQAELRRLKGGR"
     gene            complement(30285..32756)
                     /locus_tag="VIG70_06465"
     CDS             complement(30285..32756)
                     /locus_tag="VIG70_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY8250232.1"
                     /translation="MAEAELKTLGRYNIERTLGKGAMGVVYEGLDPKLNRRVAIKTIL
                     KSHLDPDTAKDYSMRFSREAQAVARLNHPHIVQVYDFAEEGDIAYLVMEFIRGKELKN
                     FFDANERFDIREAVRIMCELLDALEFAHNAGIIHRDIKPANVMLDAQARTKLTDFGVA
                     RVQDVDRTHAERTQAGTMVGTPAYMSPEQITGASIDKRTDVFSAGIILYQFLTGEKPF
                     TGGGAWTIAKKIIQEDPPLPSSLNNAVTPYFDAVVNKALSKNPETRYPSAKDFAVALK
                     RALQGQGEEDDSDKTVVGEMSLPPAARSAPQPRPAAAVGGSGTQTSTQAGNQEVELEF
                     WRAIKDGNDPDDFELYVQQFPTGIYAALAKRKIAKLRGATPEETTAEAKKQERKEAEE
                     AASREADARAKLAAEKEKLELELKRKEEEFQKREAEREAEAQARAVAQAQAEKARLEA
                     IEKAKQEASAALEAERERARLEAEKARQEAENAKREVEEKVKKENEALAKKQAEERAK
                     REAEFKKREAELKAQARGKKTSPAVLLGVPAVIIALAGAGWYFFAPDDAVQKARIAAM
                     QRQLEDAKRQAEQAEKAKKDADDARIALERARKEEEAARKSGDAKQKQLAEEARARAE
                     SEAKKTAEASRLRDAEAKKTADAAKAATDKASAEERTKAKLAQEKAEKDRAAAEKAQA
                     AKLAADRAAAEKALADAKAAAEKAKADADKAIADARAAEAKAKADAEKAKREAATTTV
                     ASAPAGSDTLFEQASALEQQGKGGEAVKAYTRAARAGNSKAMKRLGEIYDKGIPGVSR
                     DYAESLKWYNAARVLGEEVPLAKGR"
     gene            complement(33008..33895)
                     /locus_tag="VIG70_06470"
     CDS             complement(33008..33895)
                     /locus_tag="VIG70_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8250233.1"
                     /translation="MAEQASEAAVLFADVSGSTKLYETAGNAVAHAAVESCVNVMRQK
                     TQAAKGRVIKTIGDEVMSAFSSADDAADAAIEMQLAISELPPVGNTTLGIRVGFNYGP
                     VVERDGDVFGDAVNLASRLASVATKGQIITARDTVMMMSPMLKANTRAITTIQVKGKA
                     QEIQVYELMWQQSEDMTTLATHKSVFKPRNTTLRLKMQDTELVLSSERPSLAMGRDQS
                     ADLVIKERMASRAHGKIERRLDKFILTDHSANGTFVTIEGDKEIVLRREEFTLRGHGW
                     IAFGQSRTGSSDVVEFFCE"
     gene            complement(33989..>37557)
                     /locus_tag="VIG70_06475"
     CDS             complement(33989..>37557)
                     /locus_tag="VIG70_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HEY8250234.1"
                     /translation="SGAIATTTGTLTKDGAGTLTLSGTNTYTGTTTISAGNLTLQGGA
                     AIADAGVVALNGGTLAIDAAETIGRLSGAGGTSVVLDATLTFGDATDTTVASTISGTG
                     GLTKLGTSTVTLGGANTHTGTTAINAGRITLNGGAAIADAGAVTVAAGATLNLGTNET
                     IGSLAGAGDVTLNAQTLTAGADDTSTTFSGAVSGTGGLTKVGAGTLTLSGTNTYTGTT
                     NVNGGTLSLDGGTAILDAGAVVLANVAGATLNLAASETIGSLAGGGALGGNVTLNANT
                     LTTGANDGTTTYAGMISGSGGLTKQGVGTFTLSGSNTYTGATAIAAGTLTVQGGTALA
                     DTSAVTVAAGATFNVTTSETIGSLAGAGSVTLPATTVLTAGADNSDTTFSGVISGAGG
                     GALTKVGSGTMTLSGTNTYTGITNVNGGTLSLAGGTALLDTGAVVLANAAGATLDLAA
                     SETIGSLAGGGAAGGNVTLGGNTLSTGGNNGTTTYSGSISGPGGLTKQGTGSMTLIGT
                     NAYTGATAIDAGFLIVQGGTAIADASAVTVASGATLQLSNSETIGSLAGAGDVTLPGG
                     NTLTTGGNNASTTYSGVASATGGITKVGTGTMTLSGANTYTGTTTVNAGTLALGASNR
                     LADGSSLSVNGGTFELGGFSDTVNAVTLVTGTLQNGTLASGTDFALQSGTVSAVLAGG
                     VGLTKSTAGTATLSGANTYTGVTSINAGTLTASNASALGTAGGGTTIASGGTLHINGV
                     AIGAEAVSVAGTGAGGNALAWTGTSSLGGNVTLTGATTVGGTGDMTFSGTVNGGNALT
                     VNSGNNTTFAGAVGNSTALASFTTNAGGSTSINGGLIRTTGAQSYGDAVLLGAGTTLT
                     STTTGSIGFSSTINGAQSLAVNAATSASFAGAVGGGTPLASLAVTAPSISLGNVTTTG
                     AQQYNGNAVLNSTYATSGGAFGITGNTVLGSDTTISTGSGNVTFGGTINGGNSLTVND
                     SGTNNFMGAIGNTTALANITTDAPGASILGGNVTVSGAINLLDATTANGITMASTGGG
                     AITLNNANTTSPASVNTTGDVTFTSPLIGTSGTPMNFVVTPDSVTMTQLVTAFFSGPA
                     IPGTVVFPEGSSITFNGALIAQSIIQQQAAAAASTASTSVTAAIVEEANKTFGTDSVA
                     EDVEYGFAGEIGATPPMDHRIDESGISLPHCVQEAREGLPCK"
BASE COUNT         5597 a        12660 c        12613 g         6687 t
ORIGIN      
        1 cacggacaag atcgtctggg aaaacggcaa tcgctacacc ttccggctgc ggccctcgac
       61 gtacatgcag acggcgatgc tgattcccga ggcggtgaag ctgaagaaaa agcgctgggc
      121 gatcgtctat cccaactacg aatacggcca gtcggccacg gcctcgttca agaggctgct
      181 gaaggccgcg caacccgacg tggagttcgt cgccgagcag gccccgccgc tgggcaagat
      241 cgacgcgggc gcggtggtgc aggcgttggc ggacgcgaag ccggatgcga tcttcagttc
      301 gctgttcgcc gccgacctgc agagattcgt gcgcgaaggc aacacgcgcg gcctgttccg
      361 gaacaccccg gtgttcaacc tgctcgccgg cgagccggaa tacctcgata cgctgaagga
      421 cgagaccccg gacggatggt gggtcaccgg ctatccgtgg agcgagatca acaccccgga
      481 gcacagggcg tttcgcgagg cctatcagaa acgctggaag gactatccgc gccagggctc
      541 ggtggtcggc tacaccgccg tatacgccgt ggccgaggct ttcaggaaag cgaagagcgt
      601 agaaacggag aaggtcgtcg aagctctcaa gggattgaag atgcagacgc ccttcggccc
      661 gatcgagtgg cgcgcgctcg accaccagtc tacgatgggt gcgtatgtcg gccagctggc
      721 aaggaagggc ggtaaaggcg agatggtcaa ctggcgctac gcggacggca cgaaggtcca
      781 accttccgat ggggaaataa gagcattaag gaaggagtaa cgccacagga gtgagcagct
      841 ccgccgttct gctgcagttc ctgaacgggc tcgccggcgc gagctcgctc tttctcgtcg
      901 ccgccggact gtcgctcatc ttcggcgtca gccgcatcgt caacttcgcc cacggctcgc
      961 tgtacatgct cggcatgtac ctcgccgtgt ggctctccgc cagggttggc ttctgggcca
     1021 gcgtgccgct cgcagcgggc gtggtggccg ccgtgggtct ggtcatcgaa gttcttctcc
     1081 tgcgaaggat ctatcgggcg cccgagctgt tgcagctcct tgcgaccttc gccctggtgc
     1141 tcgtcatcaa ggactttgcg ctctgggcct ggggtccgga ggacatgctc ggcccgcgcg
     1201 cgccgggatt ggcaagcgca gtcaccatcg ccggccgtca gtttccgcag tacgacctgt
     1261 ttctcatcgt gctcggcccc gcggtgttcg tagcgcttca tgtcctcctc aggcacaccc
     1321 gcttcggcat cctgattcgc gccgcgaccg aggaccgcga gatggccggc gcgctcgggg
     1381 tcaatcagcg gctgctgttt acgatggtgt tcgcgctcgg cgcggcgctc gcgggactgg
     1441 gcggcgcgct cgccatcccg cgcgagccgg cgaatctcga gatcgatctc gctctcgtgt
     1501 ccgatgcgtt cgtcgtggtg gtggtcggcg gcatgggctc gatcccgggc gccttcctcg
     1561 ccgcgctgct catcggcgag atcaaggcgt tctgcatcgg cctgggctac tcggaggtca
     1621 cgctggtcgc cgagttcatc gtcatggcgg tggtgctggt cctgcgtcca tggggcctcc
     1681 tgggccggcc gctcgccgag gcccgcggct tgacgccaac cggtgatttt gatgtggctt
     1741 cccggaatcc cctgctgata gcgctcgtgg cgctggcggt cgtcccgctg ctctccgacg
     1801 gctacgcgct ggttctcctc accgacgtgc tcgttttcgc gctgttcgcg gtcagcctgc
     1861 acctcctcct cgggccgggc ggcatggtgt cgtttggcca tgccgcctac ttcggccttg
     1921 gcgcctacgc ggccgcgctg cttttcctgc gctggcgcct gccgatggag ctggcgctgc
     1981 tcgcagcgcc gctggccgcg gcggccggcg ctgcgctctt cggctggttc tgcgtgagac
     2041 tctcggggat ctatctcgcc atgctcactc tcgcctttgc gcagatcgcc tggtcggtcg
     2101 cgttccagtg ggatgcgctc accggcggct ccaacgggct ggtcggaatc tggccggcgc
     2161 cctggctggc atcgagaacc gcctactact tcgtcgcgct cgttctttgc gccggggcga
     2221 tcgcctgcct gtggagaatc tctgcttcgc ctttcggcta cgccttgcgg ggaagccggg
     2281 attcgccgct gcgcgcaggg gccatcggca tcgacgttcg cacgcagcag tgggcggcct
     2341 tcatcgtcgc cggcagcttc gccggccttg ccggcgcgct gttcgccttt tccaaaggct
     2401 cgatctcgcc agaaaccctg tcggtgccgc gttcggtgga cgccctggtg atggttctgc
     2461 tgggcggcgt gcaaaccctc accggcccgg tctggggcgc ggcgctattc acctggctgg
     2521 aggatgccgt gtcgcgcgag gtcgcctact ggagggccgc gatcggagcc gtgatcctgc
     2581 tgctcgtgct ggctttcccg caaggcatcg ggggcttctt tggacaggca ctgacccggt
     2641 ggtcgcgcta accgtcaacc ggatctccaa agcctatggc ggcgtgcagg ccgtgcgcga
     2701 agtctcgttc agcgtcgctc cgggggaaat ggtcgcgatc atcgggccga acggcgccgg
     2761 caagaccacg tgcttcaaca tgctgaacgg gcagctcgtg cccgactcgg gcgagatccg
     2821 gctcggcgac gcgagcctgg ccgggctgcc gccgcaccgg gtctggcggc tcggcgtcgg
     2881 ccgcaccttc caggtcacgg ccaccttcgg ctcgatgagc gtgcgcgaga acgtccagac
     2941 cgccctgcgc tctcatcaca agaaaaacta ctccttatgg agtcgtgcgc gcggctcttt
     3001 cgggcaagag gcagaagcgc tgctcgagcg cgtgggcatg cgcagccagg ccgagcggcc
     3061 ttgcgcggtt ctcgcctatg gcgatctgaa gcgcgtcgag cttgcgatcg cgctggcaaa
     3121 ccggccgcgg ctcctcctca tggacgagcc gaccgcgggc atggcgccgg aagagcgcgg
     3181 ggccctgatg gctctcgccg cgaccctggc gcgcgacgac cgtctcgccg tgctcttcac
     3241 cgagcacgac atggacgtcg tcttcggatt cgccagccgg gtcatcgtca tgcacctcgg
     3301 cgaagtcatc gcggcgggca cgccggccca ggtgcgagcg gatgcgcgtg cacgcgaggt
     3361 ctaccttggc gacgcttgat atacgtggcc tgagcgcggg ctacggccgc gcccaggtcc
     3421 tgttcgacgt cgctctggag gcgcgccagg gcgaagtggt ggcgttgctc ggccgcaacg
     3481 gggcgggcaa gtcgaccacg ctgaaggcga tcatgggctt gatcgagcct ttgtccgggg
     3541 aagtcatctt cgaaggcagg aacctgaagg acctcgagcc ccacgagatc gcgcggctgg
     3601 ggcttggcta cgtccccgag gatcggcgta tcttcacgga cctgaccgtg accgagaacc
     3661 tcgaagtcgc tcgccagccg ccgcgcggcg ccgcacccgc ctggaccgag gagcggttgt
     3721 tcgcgctctt tccggcgctc gccggcctgc gcaaccggcg cggcgcgcgc atgtcgggag
     3781 gagagcagca gatgctctgc atcgcgcgca cgctcgccgg caatccgcgc gcgatcctgc
     3841 tcgacgagcc ctccgaggga ctcgcgccgc tggtcgtcga gcaggttgcg agggccatcg
     3901 tggagctcaa gcgctcgggc atcaccgtgc tgctcgcgga gcagagccgg catttcgccg
     3961 gccgcgtcgc cgaccgcgcc tacgtcctgg aaaaaggcat catcagaggc gcatgaagcg
     4021 cggcctcctc ctcacactca ccgagccgcc gcccgggatg gaggaagaat tcaacgcctg
     4081 gtacgacgag gagcatctgc ccgaacgcct cgcgataccc ggattccgct cggcgcgacg
     4141 ctgggttgcc ggcaggacgt acctcgcgac ctacgagctc gactcgcccg aggtcctctc
     4201 ttccgcgccc tatctcgcgc gctaccggaa tcagacgccc tggtcgcgcc gctgcctcgg
     4261 caagtgcgtg acgttcaagc gctgggcgtg cgagcagctc gacccgggcg acgccgaccc
     4321 gcacccgggc gcgaagggct tgattctcgc catggacgcc aggtcgcccc ctcgcgtggt
     4381 ccaggcccgg cgcttcgccg ccgactccgg cgaaagaatt acgctctacg agctctccga
     4441 tcccgacccg actggctacc gcctctacaa gtcttgagcg ctacgcctgg ctgtccatcg
     4501 cggcggcgct cgcgacgatc ggcctgaaga cggctgcgtg gtggctgacc gggtcggtcg
     4561 gcctgctttc ggatgcgctg gaatcgctcg tcaacctcgc ggcggccatg ctcgcgctgt
     4621 ccatgctgcg cctcgcggcc tcgccgcccg acgaagacca tccctacggc ttctccaagg
     4681 cggagtactt cgccgccgga atcgaaggcg ccctcatcgt gctcgccgcc ggcgggatcc
     4741 tcgccgccgc catcccgcgg ctgatcgagc cgcgcgcgct cgacgcgccg atactcggcc
     4801 ttgcgctgac cgtcgcggcg tcggcgatca atttcgcggt cgccgtggtc ctggtccgcg
     4861 tcggccgcag ggagcacagc atcaccctcg aggccgacgg ccagcacctg atgaccgacg
     4921 tctggacctc ggcgggcgtg atcattggcg tcgcggccgt gcttgccacg ggctggctgc
     4981 gcctcgaccc gctggtcgcg ctcgcggtgg ccgcgcacat cgtgtggacc ggcctgcggc
     5041 tgatgcgccg gtcatggaaa ggcctgctcg acgccgcgat ctcgaccgag gacacgggcg
     5101 aggtgaccaa gcttttcaag gaatattcgc gacgtcaggg cgtcgcattc cacgcgctgc
     5161 gcacgcgcca ggcgggcgca cggcgcttca tctcgttcca tctcctggtg cccgatgcct
     5221 ggagcgtggc gcgggcgcac cagctttccg aggaaatcga atcgcgcatc cgctcgatgg
     5281 tgccgaacgc cgccgtcttt acccacatcg agccgatctc ggaccccgct tcgtacgacg
     5341 accaggggct cgatcgttga tcaatgcccg ggtcagaggg ctttcgcgcg ctcgcgcagc
     5401 acgaacttct ggatcttccc ggtcgcggtg gtcggcacgg ggccgaacac caccttcttc
     5461 gggatcttga accgcgcgag gttctgccgg caccagtcga tgtagtcctg ctccgtcgcg
     5521 cttgcgccgg gcttgagctg gacgaaggcg cacggcgtct cgccccactt ctggtccggc
     5581 cgcgcgacca ccgcggcgag ctgggtcgcc gggtgcttgt agagcgcgat ctcgacctcg
     5641 accgacgaga tgttctcgcc gcccgagatg atgatgtcct tcttgcggtc cttgatctcg
     5701 acgtagtggc tcgggtgcca caccgccagg tcgccggtgt gcatccatcc gccggcgaag
     5761 gcttccgcgg tcgctttcgc gtccttgaga tagccgagca tcacggtgtt gccgcgcacc
     5821 aggatctcgc cgatcgcgct tccgtccttc ggcaacggct tgcaggtttt cggatcggcg
     5881 acgatgtggc cctcgacgac cggatagcgc acgccttgcc gtgccatttc ggtgtacttg
     5941 tcgtcccagt cctgcggcgc gcagaacgtc gacggcccct ggagctcggt catgccgtag
     6001 atgtgcagca cctggaatcc gagctcctcc atcgcctgga tgaccttggc cggcggcggg
     6061 gagcccccgg tctggatgtg cactgggtgc gggaacttgc gccgctcccc ttccggcgct
     6121 gcgatcagca tgcctaatac cgtcggggcg ccgcacatgt gggtgacgcc atgctccacg
     6181 atcaattcaa aaatccgctt gggctcgacc gcccgtaggc aaacgtgcgt gccggccatc
     6241 gcggtcaccg accaggggaa gcaccagccg ttgcagtgga acatcggcag cgtccacagg
     6301 tataccgggc tcgcgggcat ggcccacgcc atgacgttgc cgcaggcctc gaggaaggtg
     6361 ccgcgatggt ggtagacgac gccttttggc ctgccggtgg ttcccgacgt gtagttgagc
     6421 gcgatggggt cggattccga ttgcggaagc gaccattcga acccggggct tccggtcttg
     6481 agaaaagctt cgtattcgac ctggccaagc cgctcgcccg gtccgccgta ttgcgggtcg
     6541 tcgacatcga cgacccgcgg cttcttcttg agcttggcga gcgccggccc gcaaacaccc
     6601 gcaaacatgc gatcggtaat gaataccttg gcttcgccat gctcgaggat gtaggcgacc
     6661 gtatcggcat ccaggcgggt gttgatggtg ttcagcactg cgccgagcat cggcaccccg
     6721 tagtgcgcct ccagcatggc cgggatgttg ggcagcatgg ccgagaccac gtcgccacgc
     6781 ttgacgccgg ccttctgcag cgccgaggcg aggcgctttg cgcgctcgaa aagttgcttg
     6841 taggtgaacc gggtagagcc atgcaggacc gcaacgcggt cgggaaagat ctctgcggcg
     6901 cgcggcagga agacgatcgg tgagagggcc gtgtagttcg cgggacctgg cttcagggac
     6961 gaccacatgc cgtttttgcg cgttgcagca ttcgctttac gattcttcgg tcggcgcttg
     7021 gccattcgct tcccctcgct ttagactcgg cgaatctact ccaaggagga ggaatccgtg
     7081 aagacactgg ccatcgcagt ggcgttggcg ctttccacga gcgcctttgc gcaggagaag
     7141 tcgatccgca ttgccggttt tggcgccaag tcgggcgtgg tgcgctcgtt cggcatcaac
     7201 agcgaagcgg cgatgctcgc cgcggcggac gtgatcaaca agcagggcgg cgtcaagctc
     7261 gccgacggct cgaaagcgaa gctggtgatc gattacctcg atgaccgctg caccgcggag
     7321 gaaggcatct cggtgctgcg ccggatttac ggcaccgatg ccatcgtggc ggtcggcccc
     7381 acctgctcca acgtggccga gcctctgttc ggcatcctgc agaagaaggt cgacgacaag
     7441 ggcgactcgg gcctgcagat gccggtgttc accgacgtcg cgatcaagat cggcctcgcc
     7501 aagatctcgg aatgggcgtt ccgcaacgtg cccaacgaaa gcatcatgta ctcgtcgctc
     7561 ttctactggc tgaagaccaa gtatccgaac gcgaagacgg tcttcggcgg cgtggaggag
     7621 gatttcgcgc actcgcgcgc gtcctggtac gcgatcatga aagaccgggc ggaggcgaac
     7681 ggctaccagg tgctcggcaa ctcgaagtgg ctgctgaacg acaccaactt cgccacccag
     7741 gtgcgggaaa tgaagaacgc caaccccgac atcgtcgcga tctcggcgca cccgttcacc
     7801 acctgcggcg tgctgaagga gatgcagcgc cagggcgtga agcccaaggt gctggtggga
     7861 ctcacgagct cctcgagcct ggagacgctg caaggctgcg ccaagcaggc cgagggcatg
     7921 atcatcccga cctcgttcgc cccggtcacc aaggaaggca agaccgcggc cgagaccacg
     7981 gccaagttca agggcgcggc cgacctgcac tcgatggcgg cgtgggagaa catgttcatc
     8041 ctgaagcagg tcatcgagtc cgagaagatc tccggcgaca agagccagct gcaggccgac
     8101 cgccgcaaga tccgcgacgg cctcgccaag ctgagggaga ccaagggcct catgggcgtg
     8161 acgaagcgca ccgacgatcg cgaggccgac aagccctacc tcttcgtcca ggcgaacaag
     8221 ggcgaatgga aggtgctgca taatcctctc taagggtttt tgctcgactt cctccaggcc
     8281 gtctgcgacg gcctactgtt cggcgcgacc tatgcgctga tcggtatcgg cttcacgctg
     8341 gtgttcggcg tgatgcacaa gctgaacctc gcgtacgccg cggcctcggt cggcgcggcg
     8401 tatgcggggc tcctgatcct gcagcttgcc gccggcgccc cgccgtgggt ggtgttcctg
     8461 tgtgcgttcc tcgggggcgg tgtcatcggc tggctggtgt acgtcacctg cttccatttc
     8521 atcccgcttt cgaatcagct cgcgccgctg atggcgacgg tcgggatgct gctcctgatc
     8581 gacgagatca tcgtgcacct cacagcgggc atgccgcaga actacccggc gatgttcgac
     8641 gacgtaatgc tgcgcgccgg cgagttcacg ttgcgtggcg acctactgct ggtggccggg
     8701 gtgtcggtgg tctgcatgat tgcgcttctc gccctgctct accgcacgcg cctcgggctc
     8761 gccacgcgcg cggtttcgca gcaggccgtg gcagcgcagc tctgcggcat ccgcgtcggg
     8821 caggtgaacg ccatgacctt catcgtcacc ggcttgctcg gcgggatcgc cggcgcgctc
     8881 atcggcgcct cggtgggagc gctgtcgccg cttctcaccg cgccgctcac ggtaaagggc
     8941 ctgatcgtca ccgtgatcgg tggcctgggc agcatccccg gcgcgatcgt cgccggcctg
     9001 ctggtcggcg ccttcgagaa tgtgttccag cagttccgcg gagtcactga gagagatctt
     9061 tacgtgatgc tgctgctttt cgccttcctc atcttccgcc cttcgggact cttcaagcag
     9121 taatggactt ctattacggc ctggcgcaga ccgtggggat tcacaccctc ctcggacttt
     9181 ccgcctacat tgtgctgctc accgggcagg tcaccctggc gcaggcggcg ttcttcgcca
     9241 tcggcgccta cgccgccggc atgctcaccg tgcttgcggg atggcacatc gtgccggccc
     9301 tcggcgccag cgcgatgttc ggcgcggcga ccgcgtgcgc ggtgggcttt cccgccctgc
     9361 gcgtgaaggg gttgatgctg gtcgtggcca ccctcgcctt cggtgaaggg gtgcgcctct
     9421 tcttcttcaa cttcgactac caggtcgcga aaggcgagat caaggtgggt ccgctcggcg
     9481 gcgaaggctt ccgccagatc cgctacttcc aggagcacgg ctggagcacc ggagaagtga
     9541 tgctgttcgt ctgggtcttc gtcgtcctgg ccatggcggc gctctggtgg ctcgaccgct
     9601 cgcgctacgg cgcggtgctg cgggcggtcg gcgaggacga gctcgccgca caatccgtcg
     9661 gggtcaacct caccgcggtg aaggtgagcg cgatgaccgt cggcggcgcg gtggcgggta
     9721 tcggtggcgg cctgtacgcg cattacacga cgcacatcga gcacgcgaac ttcaacgtgc
     9781 tcctcgccac cttcgcgatc gcctatccga tcgtcggcgg actgaagaac gtcttcggca
     9841 cgctcgccgc ggtgatcttc gtgcaggcct tcctcatcga agggctgcgc ttcctcggcg
     9901 actggcgcaa cctgctgttc ggggcgctga tcgtcgcggc gatgaacctg cggccgcagg
     9961 ggttccttgg gggccggcgt gcttgagttg aaagggcttt cgcgccactt cggcggcgta
    10021 aaggcggtcg acggcctcga cctcgcggtg cgccaaggcg agatcgtcgg cctgatcggc
    10081 cccaatggct cgggcaagag cacgaccgtg aatgtgatcg ccgggctctt tcccgtcacc
    10141 gcgggtgaag ttcgtttcaa caacgaaagt ttggaagggc taccaaccca caaacggctg
    10201 cagcgcggca tcgcgcgcac tttccagaac atccggctct tcggccagct ctcggtctgg
    10261 cagaacctgt gggtggcgca gaatgctccc gaggaccatg cgcgccgcgg cttcttcgcg
    10321 cgctggctcg gcggccagtc cggagtgcgc gccgagatcg cgcagctcct cgagttcagc
    10381 gggctcgccc acaagcagga cgagctggcg gcgaacctcg ccttcggcga gcagcgccgg
    10441 ctcgagctcg cccgcgcgct ggccgcgaaa ccgaagctgc tcctgctcga cgaacccgcg
    10501 gccggcatga acagcgagga gatcgggcag cttcgcgacc ggatcctgaa gctccgcgcc
    10561 cagggcatca cgatcctgct gatcgagcac gtaatggagc tggtgatggg ggtgaccgac
    10621 cgcatcgcgg tgctcaactt cgggcagaag atcgccgaag gcagccccgc cgagatccag
    10681 gcggaccccc tggtgagaaa agcctacctg ggcggagctt gatgctgcgc gttcaggatc
    10741 tcgttactgc ctacggcaag atcgaggcgc ttaagggcgt gtcgctcgag gtggcgcagg
    10801 gctcgatcac ctgcctgctc gggcccaacg gcgccggcaa gacgacgttg atgatgacgc
    10861 tcgccggaat cctcaggccg aggagtggct cgatccgttt ttcggaagat gagattacgg
    10921 ggttgccgcc gcacgcgatc gtcgcccgcg ggatcgccct cgtgccggag aaccgcctcg
    10981 tctttcccag catgacggtg ctcgagaacc tgcaggcagg cgcctaccag aggcggaacg
    11041 gcttcgaaga cgacatcgag cgcatgttta cgcgcttccc gaggctcaag gagcgcgaag
    11101 cgcagcccgc cggcacgctc tccggcggcg agcagcagat gctcgccgtg gcgcgcgcgc
    11161 tcatgtcccg gccgaagctc ctgctcatgg acgagccttc gctcggcctc gcgccgctgg
    11221 tggtggagga gatcttcgcc atcatcaccg agctgcaccg cgacggcgcc accatcttcc
    11281 tcgtcgagca gaacgcgcac atggcgctca gggtcgcgca gcgcttctac ctgatggaac
    11341 agggccgcgt caccttcagc ggcgacccgg gctctcttgc ggaggatgaa gtcatccggc
    11401 gggcctatct tggctccagt cgttgatttt cttcagaaca gcctcgacgg cctgagcgcg
    11461 ggcgcggcat acgctctcct cgcagtcggc ttcacgctga tcttcggggt cctgcggcgc
    11521 gccaacctgt cttacgggcc ggcgatcatg ctgggcgcgt atgcggcgac ctggctcttc
    11581 ctgcaaaccg gcgccgggct gctggttctt ttcgccgtgg cggtgctggc gacggtcctg
    11641 gcgggaagct atgtcgagcg cctttgcttc gctccgcatc gtaggggcgc cgcgacggcg
    11701 gcgatggtgg cgagcttcgc gatctggatg cagctcgagg agcttgccac actgctgctg
    11761 ccgcggcacc tctacgcgtt tcccgcgctt tacgagggcg gcccgctgac cttcgggccg
    11821 ttcttcgtgc gcgtggagtc gctcctgatt ctgctgagcg ccgtcgcggt gtgcgtcgcc
    11881 ggctggaaag tcctctatgc gacgcgcttc ggtattgcgg tgcgcgcggt gatcgatgat
    11941 ccccgcgcgg cggagctttc gggaatcgat gtcggcgcgg tgtcgctgcg catcttcgcg
    12001 gtcgcgtcgg ccatcggggg cgcaaccgga ttcctgatcg tcgcgagcca cgagcaggtg
    12061 acgccgatgc tcggcatgtg gtcgactctc aagggcatgc tcgcgatgat gctcggcggg
    12121 ctcggctcgc ttcccggcgc gattgcgggc gggctcgcgc tcggcctgat cgaggcgcac
    12181 agccagtggt acttcggccc gcagttccgc gacttcatcg cttacctgct gctcttcgcc
    12241 ctcctcgccc tgaggccgcc atggatgaat acgcgatcgg cgtattgacc agcgtcgggc
    12301 tgctgtcgtt cgtcgccacc tcggcctacc tgttgctgat cgcgggcgag ctctcgttcg
    12361 gccagcaggc ctttttcggc gtcggcgcct acgccgccgg gattgccact gcgatgctcg
    12421 gcttgccgct tttcctgggt cttctcatcg ccatggtctg cggcgccgca gtcgccgcgc
    12481 tcgtcggcct gccggcgctg cggttgcgcg ggctctattt cgccatggcg accctcgcgg
    12541 cggcggagat gctgcgcatc ctgttcgagc tgttccactg gcaggtcgag gtcggcggag
    12601 agctcgtcgg ccccgacggc acccagggct ttcgaggcat ccggtgggtg ttcgagcgcg
    12661 gcgtcgagcc gctcggtttt ctggcgttgg tttggggcat gcttgcggtc gtgctcgtcg
    12721 tcattcacct ccttgagagg acaagactgg gcagcgcggt ccgcgccctc ggcgaggacc
    12781 ccgagctcgc gggcacgctc ggcatcgacg tcctgcgcct caagctggcc gtggtcgccg
    12841 cggccggcgc gctcgcggcg ctcggcggcg cgctcttcgc gcatcacaat acctacatcg
    12901 agccgcgcaa cttcgacatc atgctcggcg tgcatacgct tgcttacgcg ctcatcggcg
    12961 gcctgggcac cgtggtcggg ccgctgctcg gcgtcgcgct cgacctcggc ctcctcgagg
    13021 gcacgcgggt cttccagggc taccgcatga tcgtgttcgg cggcctggtt gccctgctgc
    13081 tcatctggcg cccgcgcggc ctgctcgacg agcgcgtggt gcactggctg aggacacgaa
    13141 gatgagatgg ctcctggggc tcaccatggt cctttcgttc gaggcagttg cccaaaaagc
    13201 cgaagcgcgg ctcgactgca ttcacaccgg caaggatttc atttacgact gcgttatcga
    13261 gatccagaga cagggactac ccctcaccga cctggacgtg cggatgagcg cggacatgcc
    13321 gtcgatgccc atggcgcacc aggtgaagcc ggtcagggca gacgcgacca gcacgcccgg
    13381 tgagtatcgc gcgcggctcg acctggagat gggcggcgaa tgggcggtga agctgaggct
    13441 tgccggcccg gtgcgcgacc agctcgtgct gcactacgaa ttcgacgagc gcggcgcgcg
    13501 tccggtcaga ggatccggca agcctcctcg aaagtgaggc gcgggttgcg cggcggcagg
    13561 ccttggccgg tgccgtagcc gatgttgatg aggaagttcg acttcaggcg cccatccggg
    13621 aagaaggcgg cgtcgagggt agcgttgttg aagccggaca ttgcgccgca atcgagcccg
    13681 acggcacgca gggcgatgat caggtaggcg ccctgcaggc tcgagttgcg gaacgcgttg
    13741 ggctcggcga cggcaggctt gttgtaccag ttgatcgcgt cggggttgtg atagagccgc
    13801 ggcaggtgct cgtagaaccg cgtgtcgtag gcgacgagcg cgaccagcgg cgccttcatc
    13861 gtcttctcca cgttgaccgc cgacagcgcc gggcgcagct tctcccgcga ttccttcgag
    13921 aacagataga agatccgcgc cggctggctg ttggcggtgg tcggccccca tttcatcagc
    13981 tcgtgcgcct tgcgcagcag cgcctggtcc accggccggt cgtgaaagtt tccatgcgag
    14041 cgcgcctcgc gcaggatcac atccagcatt ttttcgtcag ccataatcgc ccccatggtg
    14101 gagctgagta gtgtaattcg ccccggcgat gggattctgt ggggacaggc ctgcgccgag
    14161 ccccagacgc tcgtggaagc gctggtgtcc caacgcggcc gcctgggcgg agtgggatgc
    14221 ttcctcggct cgagctactc cggcatcgtc aaggccgaac acgccgatga cctgacgctc
    14281 tcctcctatt gcggcatcgg caccaaccgg gcgcttgcgg atgccggcgt gctcgggatc
    14341 ctgccggtgc cttattcaca gcttgggtca ctgattcgtt cacgggcaat cccggccgat
    14401 gtggtcctgg ttcaagtgag cccgcccaac gcgcgcggcg aatacagcct gggccttgcc
    14461 gccgactacc tggtgccggc gcttgacacc tgccgggcga tcgtggccga ggtgaacgag
    14521 caggtgccgt ggacgcacac cgagcgcctg ctcacgaggg ccgatttcga cctcgtggtg
    14581 gaaagctcgc ggccgcccgc cacgcctgca gcgcgtccgc cgggtgacct ggagcaggcg
    14641 atcgctcgca acgccgctgc cttcgtcccg gacggcgcgg tgctcgaatt cggcattggc
    14701 gccctgcccg acgccgtatg cagcgccctc ggccgccacg agggcttgcg ggtgcactcc
    14761 ggcagcatcg gcgacggcgt gatcgagctc gcggagcgca ggatcgtgag ccgcatcgac
    14821 tgcgcgatgc tgatcggctc caggaagctg ttcgacttcg cgcgcgacaa cgaccgcgtg
    14881 cgcctgcgca gctccgagta cacgcacaat gccgaagttc tcgcccggca gccgcgcttc
    14941 gtcgcgatca actccgcggt cgaggtcgac ctgaccggac aggtcagcgg cgagctcgcc
    15001 ggagcgagct atgtgggggc ggtcgggggc gcgctcgact tcgtgcgcgc cgcgaaccag
    15061 tcagccggcg ggattgcgat taccgtaatc ccaagctcga gaatcgtaga acgtatctcc
    15121 agcaccgtgt cggtgccgcg cagcgaggca ggggtcatcg tgaccgagaa aggcgccgcc
    15181 gacttgcgcg gctgcggcct gcgcgaacgc gagcggcgca tgcgcgccat ttccgggagg
    15241 taactaatgc ggtggttttc aactcgacgg ccaagggccg ccgaagatca gcggaagttc
    15301 ctgagcgccg ggatggtcca gaacggcgct gctgcgagcg cgacgaagac cagcgcgaaa
    15361 acgaggccga cgcggtagcc gccggtcagg tcgtgcagca ggccgccgac gaacgccccg
    15421 atcgccgcgc cgacggcgtt gctggcgtaa atgagaccgt agatggtggc gacgcggggg
    15481 ccggcgaagt agcgcgccgc gaccgacgag acgatcggcc cgcgcacgcc catgcacagg
    15541 ccgaagagcg ggacgaacag cgcgaggaga agcgccgacg gcgctgccgt catggcgagg
    15601 agcagcgcca tgccgctcgc agtgccggcg aagctcaggc tgatggtctg gcggtagccg
    15661 aagcgatccg atgcaaaccc gctgcccatc acgctcgcgg cggagagcag gccgacgacg
    15721 ccgaacgcgc cggcggcggc aagcggcgag aagcccgcgt cgaccagaaa gacgacgagc
    15781 tgcacgatga tggagaacat ggcgatcgaa gtgcagaaaa acacctggac caggccccag
    15841 aacagcggcg cgcgcagcgc cgccgccagc gtccagcccg cgccggcttt cccccttgcc
    15901 tcggcgcgtg cagcggggtg gcccgcggca tacgttttcc aaggcaagcc gaacacgatg
    15961 agcggcgcca acagcagaag cgccccaccc atgaggcggt aggtcgcgcg ccagtcaagc
    16021 tcgccgacga ggtactgggc aagaggaatg aagatcagcg cgccggcgcc gaacgccgag
    16081 tacgcgatgc cgatcgcgct cgacaggcgc tcgcgatacc agcgggcgag cagtgccgag
    16141 gccggcacca tcccgttcag gctgacgccg atgccgacca gcgcgccgat gaggaggtag
    16201 aactgccaga ggtccacgag cccgcccgcg aggaagaacg ccacccccag gctcgccatg
    16261 ccggcgccat agacccagcg cggcccgagc cggtcgaaga ccaggccgac cagcggcgcg
    16321 gtaaggccgt tcaccagcag gtacaccgag tagacgccgg tgagctcgga gcgcgtccag
    16381 ccgaactcgc gctcgagcgg cagcaggaac acggcatagg tgtcgcccag cccgcgcccg
    16441 aacaggttca ggacgaagca ggcggcgaga acggcgagcg gtgtactaag tcttttcggc
    16501 aggttcgagg ccgaagacct gcctgaggta gcgcaggtag ccgggatcct tgctgatcgt
    16561 cttgcccggc gcgtcggaca ccttggccac cggctggccg ttgcactcgg tcatcttgat
    16621 gacgatgttg atcggctcgt ggccaaggtc gttggtcagg ttggtgccga tgccgaacgc
    16681 agtgagcgcg cggccgtgga agcggcgcgc gatctcgatg gcgatcggca cggtgagctg
    16741 gtcggagaag atcagcgtct tggtgcgcgg atcgacgcgg ttcctgcggt agtgcgcgat
    16801 cacgcgctcg ccccacgcga acggatcgcc cgagtcgtgg cgcgcgccgt cgaacagctt
    16861 gcagaagtac atgtcgaagt cgcgcaggaa ggcgtcggtg ccgtaggtgt cggccaccgc
    16921 gatgccgagg tcgccacggt attcctgcgc ccacttgtcg aaggcgaagg tctgcgagtc
    16981 gcgcagccgc gggccgagcg cctggcaggc ctgcatgtac tcgtgcgcca tggtgccgag
    17041 cggcgtcagg ttgttgcgca tcgcgaacca gacgttcgag gtgccggcga agttctgcgg
    17101 gatgtcgcgc ttgaaggttt tcagcacctc ctcttgccag tcgcgcgaga agcgccgccg
    17161 ggtgccgtag tccgagatct tgaagccgag ctccggctcg accgcgcgcg ctacgccgat
    17221 cttggccttg aggcgccgcc gtccttcggc gaggttgggc ttgggctgcg tgcggcggaa
    17281 atagagctcc gagacgatcg cgagcagcgg gatctcgtac atgatcgtgt gcagccacgg
    17341 tccccgcacc gtgatgtcga tctcgccggg cttctcgccg gctcctaagc gcacgtacct
    17401 ctcgctgaaa tgaaacagcg ccaggaaatc gacgaagtcc gacttcatga acctcaagcc
    17461 ccgcaggtag gttagctcgt cgtcgcggaa gcgcaggctg cacagcttga cgagctcggc
    17521 ctcgatcgcg ggcaggtccg gcctcagatc caccccctcg gtgcggcact tgaacttgta
    17581 ctcgacctgc gcgcccggaa agtggtgcag cacgacctgc atcatggtga acttgtacag
    17641 gtcggtgtcg agcagcgact ggatgatcat gccgcggcca ttgtaacctc acgcgcatgt
    17701 gtggccgcta tgcgctgcat gcgaatcccg acgtcgtcgc gctgcagttc gggctcgagt
    17761 ctccgcctga attcctgccg cgctacaaca tcgcgccggc gaccgacatc ctcgtggtca
    17821 ggaaccatga agcggcgctc gcgcgctggg gcctgcgcgg ccgtttcgtg aatctgcgcg
    17881 cggagacggt cgcttcgaaa ttcaaggcga gcgcacgctg tctcgtgccg gtgagcggct
    17941 tttatgaatg gaagagggaa ggcggccgca agcagccctt ctattttttt ccggagaacg
    18001 agccgctgtt cggcttcgcc gcgctgtggg agcgcgacac ctgctcgctc atcaccaccg
    18061 aaccgaatgc cgtcatcggc aaggtgcatg accgcatgcc gctcatcgtc cccagggagg
    18121 gctacgcggc atggctggcc ggggatgaaa gcattctggc caccgcgccg gaaatcgcca
    18181 tgctcgcgca ccccgtgagc atggcggtga accaggcggc gaacgactcg ccgaaactga
    18241 tcgagccggt cgagctagcc cccgggctgt tcgactagcc cttgtacgcg tacgccgagc
    18301 agcctcaccg gccggcggcc ggcctcggtg cgcgccatca gctcgacggc ccaggacgcg
    18361 atcacgtcgg cgtcgtccgt cggcatcgac agggtgtggc tgcgcgtgac ggtcttgaag
    18421 tcggcgtagc ggactttgat cgtgatggtg cgcgccgtga gatggttgcg cgccagccac
    18481 tcggcgttct cgcgcgccat gcgatcgagc tccgcacgaa tcgcttcaag ctcggtcagg
    18541 tcctgagcgt aggtgttctc cgtgctggaa gatttcgatt cgcggttggg ctctaccgga
    18601 cgcgggtcgt cgccgtgcga gagctggcgc agccagtcgg cgaggctgcc gacggtccgg
    18661 cgcaggagct ccatgtcgac cgtgcgcacc tgcacgagct tttccagtcc aatctctttc
    18721 agttttcgag ccgtgacggg ccctactccc cacagcgcct cgaccggcag ctcctgcagg
    18781 aagctttcca cccgctcggg cgagatcacg gtgaggccgt cgggcttgcg gtagcccgag
    18841 gcgatcttgg cgaggaactt gttcggcgcc acgcccgctg acgcggtgag gccggttctt
    18901 tccaggatct cgttcttcaa ccgccgggcg acgcggctcc cgaggggctc gccccagagg
    18961 ttctcggtca cgtccaggta agcctcgtcg agcgagagcg gctcgacgag cggggtgcag
    19021 gcgcgcagga tctccatcac ctggaccgac acgccgcggt agcgctcgaa gtcggggggc
    19081 accaccacga gttcgggaca caggcgcacc gcgcgcgcca tcggcatcgc cgagcgcacg
    19141 ccgaagacgc gcgcttcgta gctcgccgcg gcgaccacgc cgcgcgattc gggtcggccg
    19201 ccgacggcca cgggcttgcc gcggaggtcc ggatcgtcgc gctgctcgac cgccgcatag
    19261 aacgcgtcca tgtccaaatg gacaatgcgc cgcatatact gtatataatg ccactagtgg
    19321 acgaactgac agagcgtcaa gccgagattc tgcgcctggt gcgcgagctc accgaggttt
    19381 cggggtaccc gccgacgcgc gcggagatcg cgcaacgcat gggctttcgc tcggtgaacg
    19441 cggccgagca gcatctgcgt gcgctggaga aaaagggcgc gatcgagatc tcctcgggcg
    19501 cctcgcgcgg cattcgcgtg cgcgacggtc ggtcggccgg ccgcctggga cggctgctgg
    19561 agctgcccgt cgtcgggcgc gtcgccgccg gcagcccgat cctcgccgag cagcacctcg
    19621 acgggcgtta ccaggtcgat ccgaatctgt tcacgccgcg ggccgactat ctgctgcgcg
    19681 tgcgcggcat gtcgatgcgc gacgccggta tcctcgaagg cgacctgctc gccgtgcacc
    19741 gcacgcacga agctcgcacc gggcagatcg tcgtcgcgcg actcgccgac gaggtcacgg
    19801 tcaagcgtta ccggcgccgc ggccatgcgg tgcagctcga agccgagaac ccggaattcg
    19861 cgccgatcga agtggatttg cggcacgacg cgctcacgat cgaaggcgtc gcagtcggcg
    19921 tgatcaggaa cggcaagact ttctaggtct tagttccgct ctcgcgggac tcagggataa
    19981 ccccccgggg ctttgcctat agaattggcg caggttccct tctgcgccgg atgaaccact
    20041 ccacgaccgc caacggcacc aacaccgggc tcgaggtctc gacccacgcc gtcgaggcgg
    20101 agattcgcga ccgcgccgac ccccagcagg tcctcgccga ccgcgtggcg cagctctacc
    20161 ggcagatgcc gatcgcgatc ggcgcgacct tcatcgcggg cctgatcgca accttcgagc
    20221 ttcagggccg gtggctgaag gagctggtgt tgatctggtg ggggctcgtg gttgcctaca
    20281 gcgttgccgc cagcctgctc ctctacgcct accaccgcag cgcggacaag gtcaccaccg
    20341 cgcccgagtg gctgcgctgg ctcggtatcg cagcgctcgg caacggcgcg agctgggggc
    20401 tcgccggcgg ggtgttcttc cgctcgctgt cggatgagca gcaggtattc ctcgcttttc
    20461 tcttcgccgg catggcttcc gtcggcatcc cggtgtatgc cgcctcgtgg ccgatcttcg
    20521 cgctctacgc cgcgggcatc ctcgggccgt tcttctacgt cctgctcacg ttcggcaatc
    20581 ggctgttcgt ggagatcgcg ctcctcgtgc cgctgttcta cgtcatcaac gtcgccatcg
    20641 cctaccgcct gacgcacgtg ttccattccg gctaccgcct gcgccacgct tatggaaagc
    20701 tggcgcagga ccaccgcttg ctcaaccagc ggctcgagcg gcagctggtc gagctcgaag
    20761 aggcgcgccg ccaggtcgaa gcgagcgggc gcaagctggc gctcttcgcc gagcgcgcgc
    20821 cgatcgcggt actcgagctg cagcccgatg gcacggtcgc ggaggtgaac cacgccgccg
    20881 agatcctgtt cggctacgcc gcggccgagc tggtcggcgg cccagtgaag aagctggtgc
    20941 tggccgagtt ccacgacgag ttcgaccgca tctggctgca gctcctgagc tcgcgcgagt
    21001 cgatcgccgg gctcaagatc cgcaacccgc ggcgcgacgg catcgagctc acctgcgagt
    21061 ggacggtgac cccgctggtc aacttccaga aggacgtgat cgccgtgatc gcgcagggcc
    21121 gcgacgtgac cgcgcagctc gaggcggagc gcatgaagaa ggaattcact tcgacgctct
    21181 cccacgagct gcgcacgccc ctcacctcga tcatcggctc gctgcagctc atcaacagcg
    21241 gcgtgatggg cgacgtcgac aaggaggtcg gcgagctcac catggtcgcc gagcgcaacg
    21301 gccagcgcct gctcgacctg atcaacgaca tcctcgacat cgagaagatc gagtcgggca
    21361 aggtgacgct cgcgcccgag gtgatgcggg tggacgagct ggtgcgcgag tcgctggtgc
    21421 tcaacaaggc tttcggcgag cgcttcaagg tgagctttca gtccgatggg gacattccgg
    21481 agcgccaggt gcacgccgac cgcaagcggc tgctgcaggt gatgaccaac ctgctatcga
    21541 acgcggcgaa gttctccccg gaaggcggga tcgtcgagat cagcaccgag gatgccgggc
    21601 ccaatgtgcg catcgcggtg cacgaccgcg gcagcggcat ccccgaggcg ttccgctcgc
    21661 gcatcttcgg ccgcttcacc caggcagact cgaccgcgac gcggcagaaa ggcggcagcg
    21721 gcctcggcct cgccatctgc aagcggctga tcgagctgat gcggggccgc atcggcttcg
    21781 acgaccgccc ggggggcggc actacgttct ggttcgagct cccgcgccgg acagcgtagt
    21841 atcgagaata tgccggcccg ccccctgaac cgcatctgct acgtcgagga cgacgaggac
    21901 atccagcgca tcgtgcgcat gtcgctcgag cgcgtcggca agatgaccgt cgccgtggtg
    21961 ggcgacccga cgcaggcgat cgcgatgatc accgagttca accccgacct ggtgatgctc
    22021 gactggatga tgccggtgat ggacgggccg acgctgttcc gccagttgaa gctgcgtcct
    22081 gagacgagcg cgctgccggt ggtattcatc accgcgcggg cctcgcaacc cgacctcgac
    22141 gagctgaaga ccctcggcgc ggccggcacg atctcgaagc ccttctcgcc caaggacctg
    22201 cccgaccagc tgcgggcgat ctgggcggcg ctgccttagt gcaggacctg ggtcttgtcg
    22261 cccggctcgg cgggcagctg ctcgagcttg cgcgccagga agccgatcac ctcgtcggcg
    22321 ttgcggaagc gcgcttcgcg gtccttctcc accagctttt cgatcagccg ctgatactgc
    22381 tccaggtgat ccggcagcgg cggcggctgg gtgttcaggt ggtgcatcgc cacctccatc
    22441 gcggtctcgc cgtcgaacag gtgcttgccg gtgagcatct ggtagagcag cacgcccgcg
    22501 ctgtagatgt cggtgcgcag gtcgagcgcc ctcccctgca cctgctccgg gctcatgtag
    22561 cgcggcgtgc cgaggacctc gcccgcgccc gtgcggtccg cggcggcgat gttcttggcg
    22621 atgccgaagt ccacgatcgc gaggctgccg tcgcggcgga acatcaggtt ctgcggcttg
    22681 aggtcgcggt gcaggatgcc cttctcgtgg atgtcgccga gcgcgaacat caactcgcga
    22741 aagaacttga gcgcctccag cggcggcacg ggcttgccgc cgaggcgctt gctgaggtcg
    22801 ccgccctcga agaactccat caccaggtag acgttgttct ggtcgttgcc gtggccgaag
    22861 atgcgcgcca cgtggcggct ctggatgcgc tcgaccatgg cgtattcctc catgaagcgc
    22921 tccagcgcat tcttgttcag cacgatctcc tgccgcagga tcttgaccac cagcggctcg
    22981 tcgtcgttct cgcgcgaggc gagatagacg cgcgcggtgc ccccttcgcc gatgaggtgc
    23041 agcacctcga tgcccggaat gcgcaccttg gcgccgagcg agtgggcttc catgcgcgcc
    23101 gcgaggtcgg gcgacaccgt gcgctcgacg gcgctcgcgg tgagctcgcg cagcgacgcg
    23161 atcagctttt cccgggtgag ctcctgcttg cgctggtagt cgtccgcgcc ggccttcatc
    23221 gcccgcaccg cgatgatctc gttgccggcg ccggtgagga acaggatctt cgggcagtcg
    23281 gtgcgctgct tgaggacctt cagccactcc aggccgtcgc cgcggccgag catgtaatcc
    23341 aaaacgatga cgttgtacga gccgagcgcg aagcttgcgt cgggcaggtc gcgctcgagg
    23401 ggatcgtagg cctcgatctc ggcatccggc cactggccga ggatgtagcg cgacagcagg
    23461 ttgcgcaggt cctcatcgtc gtcgatgatc aggcatcgca tcggcgacgg actactccgg
    23521 actactcata ggtgatctgc accttggcga gatagtcctg gtattcgtcg atgctcgcct
    23581 ggagcgcggc gcggtcgccg ctgcgcgccc cgtcctcgat gtacttgccg agggtcgaga
    23641 cgtgcgtgaa cccgtacgag tttcccaccc ctttcatgcg gtgcccaagc tggcgcagct
    23701 gctcgaagtt tgcggccgcg agcgcggtgc gcagcgtgtc gagctccttg tggcgattct
    23761 tcatgaagac ggggatgagg tccgcgaggt ccttggcgac cgtcaccttg tagtcttccg
    23821 cgttcatggg cggcattata gttgccgggc cccgctcgct agtcagggat tgtcagatgc
    23881 ggatggatcg agcgtgcggc gagcatagcg aagctcgctt ccgcgaccgc gtcggcggaa
    23941 cgaaggattt cggcgaaatg cagcatcgcg ccgaaatgcc ggggctccag ctcgagcgtg
    24001 cgacggatat cgtccaggca ctcacggtcg cggccgagca tgtaatagag cgcggcgcgc
    24061 ttgtgccacg cctcggcgaa gtcgggcgcg cggcgaagga gccgggtcaa gcgtgtttcg
    24121 gcgaggtcgt agcgccgggc gacgatatcg ccggtggcga ggtcgagctc gcgcgccgcg
    24181 gcgtgatggg ggtggtgcat ccacacgtcc cagatgcggt cctcggtcgc gcccgcctcg
    24241 gcccgggcgc agcgcgcgag gcgcgtgaag agcgcgtcca ggaaggccga aacccgggcc
    24301 ggccgccggg tgcgatagct gctgatcagc ctgaggattt caaccaatcg gcaaatctta
    24361 gtggaaatcg cgcgaaaggg cagcgagcgg cccgagcagg gcgctgtagt ccgaaaggtg
    24421 cctggcgacc aagtgcacca cgtcgccgtc gcgctcgacc acgccctgca cgcccatcag
    24481 gcgcgcgccg agcagctcgc ggcgctggct ttcgaccaga ttgcgccaca ccacgatgtt
    24541 catgcacccg gtctcgtcct cgagcgtgac gaataccacg ccgctcgccg tgtcgggccg
    24601 ctgccggcag gtgaccaggc cggccacctg cgccgggctg ccatggggcc tggatttgag
    24661 ctcttcggct ctcagaaggc gcattgcgtc cagccgcttc ctgagcaatg ccagcgggtg
    24721 ccggcccagg gtgaggccga ggctcgcata gtcggcgacg atttcctcgc cctcgcgcgg
    24781 cgcctcgagc cgcggcagag gctcgggcgc aggcgcgtcg aggggcgccc ggcgcgcggc
    24841 gccggccgcc gcccagtggg cgaggcggcg atgcccggcg agcgattgca gcgcccctgc
    24901 cgccgcgaga tggcgcagat cgcgcttgct caatttcaag tcgagaacac ggccgtaggg
    24961 cttcccggcc gcaatgcggc gcccctcttt ttcgccaagg ccgctgacca tgcttagccc
    25021 caggcgcaag gcattgcctt cgagggtgca gtcccagtcg ctcgcctgca cgtccggcgg
    25081 ccgcacctcg accccgtgcc ggcgcgcatc ctggaccagc tgtgccggcg cgtagaagcc
    25141 catcggctgg ctgttgagca gcgccgcgca gaacgccgcc gggtaatggc acttgatcca
    25201 ggacgagaca taaaccagca gcgcaaaact cgccgagtgc gattccggga agccgtattc
    25261 accgaagccg aggatctgcc ggtagatcgc ctcggcgaac tcggccgggt agccgttgcg
    25321 ctgcattccg ctcttcagcc gttcttcgaa tggctccaac cccccgcttc gcttccaggc
    25381 cgccatcgaa cggcgcagcc ggtcggcctc gccgggggtg aagcccgccg cgaccatggc
    25441 gagctccatg acttgttctt gaaaaatagg aacacccaaa gtgcggctga gcacgcgctt
    25501 caccgcttcg ctcgggtaaa ccaccggctc caggccgcgc cgccgcctca gatacggatg
    25561 caccatgccg ccctggatcg gcccaggtcg cacgatcgcc acctcgatca ccaggtcgta
    25621 gaagcttgcc gggcgcagcc gcggcagcat gctcatctgg gcgcgcgatt cgatctggaa
    25681 cacgccgatg gtgtcggcct tctggatcat gcggtagacc tcgggatctt ccgccggcac
    25741 gtcctgcatt tcgatgccga tcatgctcaa agcccttcgg attgcagaca gcatccccag
    25801 ggcgaggacg tcgaccttca gcagcccgag tgcctcgagg tcgtccttgt cccactggat
    25861 cacgctgcgc tcgggcatcg cggcgttctc gatcggcacc agttccgcca gcgggccgcg
    25921 cgacacgacg aagccgccaa cgtgctgcga caggtggcgc ggaaagccgc gcagcgcgcc
    25981 ggccagttcg aagagcgggc tggagggttg cagcttctgg tcccagaacg taaagctctt
    26041 ggccagccga tcgacctcgt cgagcggcag gccaagcgct ttgcccgcgt cgcgcagcgc
    26101 gctcttggtg cggtagcaga tcaccgtcgc ggcgagcgcg gcgcggtcgc ggccgtactt
    26161 gccgtagacg tactggatca cttcctcgcg gcgctggtgc tcgaagtcga cgtcgatgtc
    26221 gggcggctcg ttgcgctccc gggagacgaa gcgctcgaac aggagattca tgcggtccgg
    26281 atccacttcg gtgatgccga gcgcgtagca gaccaccgag ttcgccgccg agccccggcc
    26341 ctggcacaag atgccctcgc cgcgcgcgaa gcgcaccacg tcgtgcaccg tgaggaagaa
    26401 aggctcgtag ccgagctcgg cgatgagcct gagctcgtgc tcgacctgcg cggccacttt
    26461 cggcgggacg cccgccggga agcgccgccg caatccttcc tccgtcagtc gccgtagcca
    26521 gctcgccggg gtttcgtttt ccgggaccag ctcctgcgga tactcgtagc gcaattcatc
    26581 gagtgaaaac cggcagcgct cggcgatcgc gaccgtctcg gcgaggagct cgggcgggta
    26641 aagctgcgcc aggcgcacgc gcagccggag gtgccgctcg gcgttcggaa acagcgcccg
    26701 cccgcactgc gcgaccggct ttccgagccg cacggcggtg agcacgtcct gcaggcggcg
    26761 gcgcgagcgc acatgcatgt gcacatcgcc ggccgcgacc agcggaagcc cgcttttgcg
    26821 agagatctct tttaaagaac ccatctttgc gcagtcgttc ggaccgcaat gcaattcggc
    26881 ggcgatccag gccgcgccgt cgaagcgctc cgccagccac gccgcggacg tgaagtcttc
    26941 tccgggaatc cagagcagca acgcgccgct tccggccaac ccctcgaggt cctcccgggt
    27001 aatggaatac cgtcccttct tgctgcggcg cctgccgatg gtgatcaagg acgagatcgc
    27061 gccgtacgag cgccggtcgg tggcgagcgc gacgagcttg aggctctctt cgaccaggat
    27121 ctcggtgccg aggatgaggt cgaggccgca ttccttggcc tcctggtgcg cgcgcaccgc
    27181 gccggcaagc gagcactcgt cggtcagagc gagcgccttg tagcccagcg cgtgcgcgcg
    27241 cgccaccagc tccccggggt gcgaggcgcc gcgcaggaag gtgaagttcg acaggcagtg
    27301 cagctcggcg tagtcaggca aaaaggccat gcaggtacca tgcctgggct tcccggtaga
    27361 tccaggcaag cgcgccgttc ggcagccggg cgacgaagta atcgcgcttc gcgtcgtcgc
    27421 cgtcccacca gcccgactcg atgcgctccg gaccggcaag cgccgtgaat tcgccttcgt
    27481 tcagcttttt tggctcgagc agccatagcg gccgcgggcc ggggacaggc gattcgcgcg
    27541 gatcccattc ccccggctcg acgcgccgcc aggcgtgctc cgggcggtga tcggggtacg
    27601 ccgcgaggcc gcatacggcc ccgtggccga ggcgcgcgcg cagccgctcg acgagccgcg
    27661 cccagccttc ggcttcactg gccggatcgc cgaacaggcc gcggctcgtg ccggggagcg
    27721 gcgcgaagtc cctggcctcc aggcggatgg aagcgacagg ctccatcaag gaaagccttg
    27781 ccaggcgttc gcgcaacacc tgcgccaggc gctcggcatg gcgcgtcgta gaagcgagac
    27841 cgacttgaac ctcggtgcaa ccgccttgcg ggtggatcag cttcaaaaga aaactgcgga
    27901 tgccctcgtg ccgcgccgcc agcaacccct cgagatgcgc cagcagccgc cgggcgccga
    27961 agagcagccc ttcggcctgc gcgacttcgg cgggcagctc caggcttgcg tcgaagtgcg
    28021 gcggcggcgc gaagaatgcg cgcggctccg gagcggcgcc gagcgcacgg tcgagctctt
    28081 cgagcacgcg tgcgccgcag cgctgcccga gcccttcccg cggtagctcg agcagcgccc
    28141 cgatcgtgct gatcccgatg cttctgaaaa attcggcgtc caaccccgcg acctcgaccg
    28201 gcagcgcctc cagggttttg ccctcgccac gcgcgagcca aagcgcagcc cttggggtga
    28261 gtgcctgagc gagcgaaaat gcaaatccca gctcggccag ccccgccacc agcttttgct
    28321 gcaagggttc aaagcccccg aacaggcgca agctgccctg cacttcgagc agcagctccc
    28381 gcggcggctc gagcgagacc ttaggcgtga actggcaggc ccaggcggcg atcggcccga
    28441 gcgcttgcgt cggcaggcgc gggaaatgaa gcgcgaccca gagcatggaa aggtcttttc
    28501 acctggagag agaaggaagc ggcggccggc gctccccggc gcttgaggat gcgaacggcc
    28561 agcgcgtcgc cgtccggatc gagcgcaatg cgcaaggcgg cgggcgagga gtcgcgcgca
    28621 gcctcgcgcg ggcgaaacag cgctacccac gccgcgcttc cttcggcggc gaccgccaga
    28681 cgcctcagct cgtcgtatcc cgcgcggcgg aaccagccga tcagcgcgtg gcagcttcct
    28741 gcgcgcagca cttgctccgc tgcccatagc gcgtcgcgcc ggccgggagc gcgcaccacg
    28801 gcgaggtgcg ccaggtccac gccggcggcg gcgagcgcag gcgcataggg cagatgcggc
    28861 ggcgcgagcc agaccacccg cttcccggcc caggaaagcg cagccaggga gggcagcacg
    28921 agctgcagct cgccgatgcc tgcctgggtg ggcaggattt cggtcagcgc tgcggccggc
    28981 cagccgccgc ccggcagagc ctggtcgagg ctagggtatc ccgttggaac tgcgggggaa
    29041 aacgctgcct tggactcgcc gagccagacc ggatgccgtt gcaggatctc ttcgagcgcc
    29101 gccatgatac tgtatacaca tacagtatct gatcagcggc cccctttcaa gcgccgcagc
    29161 tcagcctgat acgcccccat gaaatcgcgc ccgaacgcct tgttgaaatc ttcctgcgcg
    29221 tcgcgcgaga gcttttcctg cccggcgacg aacgcttccc acagcttcgc cttgcggctc
    29281 gacaggctga agcccttggc gttcttctcg aacgcgcgct cgagggcggg cgggtcgaag
    29341 cgccgcagcg cgccgaggac cgcggcgcgc atcccggcca tcaacgcgcg ttcgtgcgag
    29401 cgcaggtctt cgcaggcgtc cctgaccgcc tgcaccgggt cgaggaagcc gtcggtgcgc
    29461 tctcccgggt caaacaggaa gtccatcgcc tcgcgcggat cggacatcat cctgagggga
    29521 ttgttgtcgc gcgccgcgac catggtgcgc tcgccggtgt cgaattcctt gcgcatttcg
    29581 gcgcgcgcga gcagcagcat catgatgccg tcgatcgcgg cgcgcaggat ggcgccgcag
    29641 tcgcgcagca tccggtcggc ttccctcggc gggagctcct tgggcggcag cccggcgccc
    29701 tcgaggaacg cgcgcagcgc atcggaaggc cccgcgcccg gggccggcgg cgccgcggcc
    29761 ggctttttct gcggaatgaa ggtttcttcg ggcatcggcg cgggctgagc catcggcttc
    29821 tttgcggcga tcggttgatc gccgccgagg aacgtcgatt cgttgaacag gcgctccgcc
    29881 tggtcggcgg ggatcgccgc cggccgggcg gccgggggct gcgtcgccgg ctcggcctgc
    29941 gccgcgggcc cggactcggg cagcagcacg tggacctcgt agtcgccgag ctcgaacgag
    30001 tcgcccgact gcagcgtgag ccgtgtgccg gggccgtagc gccggccctt gaccatgacc
    30061 gggttgacct tggagaccac cgacatccag tacgtgccgc cctcctcctc gatctccacg
    30121 tggaagcgcg acatgtgctt gttcgggtct tcgagcgcga tggtgcaggc cgcgtcgcgg
    30181 ccgatgctca ccggcagcac gtcgagatcg cgcgtcgcgt agagcgtcgg ccggccgtcc
    30241 gcggcgcgct tcgtgatctt gacggtaatc aagccagatg cggcctagcg gcccttcgcc
    30301 agcgggacct cttcaccaag cactcgagcc gcgttgtacc acttgagcga ttcggcgtag
    30361 tcgcgcgaca cgccggggat tcccttgtcg tagatttcgc cgagccgctt cattgccttg
    30421 ctgttgccgg cgcgagcggc gcgcgtgtag gccttgacgg cctccccgcc cttgccttgc
    30481 tgctcgagcg cagacgcctg ctcgaagagc gtgtcgctcc ccgcgggcgc ggaggctacg
    30541 gtcgtggtgg ctgcctcgcg cttggccttt tccgcatcgg ctttcgcttt ggcctcggcg
    30601 gccctcgcat cggcgatcgc cttgtcggcg tcggctttgg ctttctcggc ggcggccttg
    30661 gcgtccgcga gggctttctc ggccgccgct ctgtcggcgg caagcttggc cgcctgggct
    30721 ttctcggcgg ccgcccggtc cttctccgcc ttttcctgcg cgagcttcgc cttggtcctt
    30781 tcctcggccg aggccttgtc cgtcgcagct ttcgccgcgt cggcggtctt cttcgcttcc
    30841 gcatcgcgca gcctggaggc ctctgcggtc ttcttcgcct cggattccgc ccgcgcgcgc
    30901 gcttcctccg cgagctgctt ctgcttggcg tcgcccgact tcctcgccgc ctcttcctcc
    30961 ttgcgcgccc tctcgagcgc gatgcgcgcg tcgtcggcgt ccttcttcgc cttctcggcc
    31021 tgctcggctt gccgcttcgc gtcctcgagc tgtctctgca tcgctgcgat gcgcgctttc
    31081 tgcacggcat cgtccggggc gaagaagtac cagccggcgc cggcaagcgc gatgatcacg
    31141 gcagggaccc ctagcagcac cgctggggag gttttcttgc cacgggcctg ggctttcagc
    31201 tcggcttcgc gtttcttgaa ttcggcttcg cgcttggcgc gctcctccgc ctgcttcttc
    31261 gccagcgctt cgttttcctt cttgaccttc tcctcgactt cgcgcttcgc gttctccgct
    31321 tcctgcctgg ccttctcggc ctcgagccgc gcgcgctcgc gctctgcctc gagcgccgcg
    31381 gaggcttcct gcttcgcctt ttcgatcgcc tcgagccgcg ccttctctgc ctgcgcctgc
    31441 gccacggcgc gcgcttgcgc ttcggcctcg cgctcggcct cgcgtttctg gaattcttcc
    31501 tctttgcgct tcagctcgag ctcgagcttc tccttctcgg cggcaagctt ggcgcgcgcg
    31561 tcggcttcgc ggctcgcggc ttcttcggct tccttcctct cctgcttctt tgcctcggcg
    31621 gtggtttcct cgggcgtggc gccgcgcagc ttggcgatct tgcgcttcgc gagcgcggcg
    31681 tagatgccgg tggggaactg ctgcacgtag agctcgaagt cgtcggggtc gttgccgtcc
    31741 ttgatcgcac gccagaactc gagctcgacc tcctggttgc ccgcctgcgt gctggtctgc
    31801 gttccgctgc cgccgacggc cgccgcgggc cgcggctgcg gcgcggagcg cgccgcgggc
    31861 ggcaggctca tctcgcccac cacggtcttg tcggagtcgt cctcttcgcc ctgcccctgc
    31921 agcgcgcgct tcagcgcgac ggcgaagtcc ttcgccgacg ggtagcgcgt ctccgggttc
    31981 ttggagagcg ccttgttcac caccgcgtcg aaatagggcg tcaccgcatt gttgagcgac
    32041 gacggcagcg gcggatcctc ctggatgatc ttcttcgcga tggtccacgc gccgccgccg
    32101 gtgaacggct tctcgccggt gaggaactgg tacaggatga tcccggccga gaacacgtcg
    32161 gtgcgcttgt cgatgctggc gccggtgatc tgctcgggcg acatgtaggc cggcgtgccg
    32221 accatggtgc ccgcctgcgt gcgctcggcg tgggtgcggt cgacgtcctg cacgcgcgcc
    32281 acgccgaagt cggtgagctt ggtgcgcgcc tgcgcatcga gcatcacgtt cgccggcttg
    32341 atgtcgcggt ggatgatgcc ggcgttgtgc gcgaactcga gcgcgtcgag cagctcgcac
    32401 atgatgcgca ccgcttcgcg gatgtcgaag cgctcgttgg cgtcgaagaa gttcttcagc
    32461 tccttgccgc ggatgaactc catcaccagg taggcgatgt cgccttcctc ggcgaagtcg
    32521 tacacctgca cgatgtgcgg atggttcagc cgggcgacgg cctgcgcctc gcgcgagaag
    32581 cgcatcgaat agtctttcgc cgtgtccgga tcgaggtggc tcttgaggat ggtcttgatc
    32641 gcgacccggc gattgagctt cggatcgagg ccctcataga cgacacccat cgcgcccttg
    32701 ccgagcgtgc gctcgatgtt gtagcgcccg agggtcttga gttctgcttc cgccattccc
    32761 ctgtgcctgt agcgggtctg ggcccgcccc cttccctatc gacccgacat tatcgagtga
    32821 tatcaaggac ctgacaagga gaaaaggcgc tgcacctgag gaacttgcgc acacattgaa
    32881 gctcatgcgc ggcaaccatc ggcgcgcgcg cctgcgactc taagcttctg ctttgatggc
    32941 tctaagcttc tgctttggcg cagtttccgc gccgccgggc cgcgcgcggc gctgacttct
    33001 ttttacacta ttcgcagaag aactcgacca cgtcgctcga gcccgtgcgc gactgtccga
    33061 acgcgatcca gccatggccg cgcagggtga attcctcgcg ccgcaggacg atttccttgt
    33121 cgccctcgat ggtgacgaag gtgccgttcg cactgtggtc ggtgaggatg aacttgtcga
    33181 ggcggcgctc gatcttgccg tgggcgcgcg aggccatgcg ctccttgatc accaggtcgg
    33241 ccgactggtc gcgccccatc gcgagcgacg gacgctcgga cgagaggacg agctcggtgt
    33301 cctgcatctt cagcctgagc gtggtgttgc gtggcttgaa caccgacttg tgcgtggcga
    33361 gcgtggtcat gtcctcgctc tgctgccaca tcagctcgta gacctggatc tcctgcgcct
    33421 tgcccttgac ctggatggtg gtgatggcgc gcgtgttcgc cttgagcatc ggcgacatca
    33481 tcatcaccgt gtcgcgcgcg gtgatgatct ggcccttggt cgccaccgag gcgagccgcg
    33541 aggcgaggtt cacggcatcg ccgaacacgt cgccgtcgcg ctcgaccacc gggccgtagt
    33601 tgaagccgac gcggattccg agcgtggtgt tgccgaccgg cggcagctcc gagatcgcga
    33661 gctgcatctc gatcgcggcg tccgcggcgt cgtccgcgct ggaaaacgcc gacatcacct
    33721 cgtcgccgat ggtcttgatc acgcggccct tggcggcctg cgttttctgc cgcatcacgt
    33781 tgacgcagct ctccaccgcc gcgtgcgcga ccgcgttgcc cgccgtctcg tagagcttgg
    33841 tgctgccgct cacgtcggcg aaaagaaccg ctgcctcgct agcctgttcc gccatctaga
    33901 accgcatgcc tccccggtgc aggggattgt acctgtgaaa aagaaacggg gcgccgcagc
    33961 gccccgtccc acaaccggga cgtgactgtt atttgcaggg cagcccttcg cgcgcttcct
    34021 gcacgcagtg gggcagagag attccgctct cgtcgatgcg gtgatccatc ggcggcgtcg
    34081 cgccgatctc gccggcgaag ccgtactcga cgtcctccgc gacgctgtcg gtgccgaaag
    34141 tcttgttcgc ttcctcgacg atggccgcgg tgacggaggt cgacgcggtg ctcgccgcag
    34201 cggccgcttg ctgctggatg atgctttgcg cgatcagcgc gccgttgaac gtgatcgacg
    34261 agccctccgg gaacaccacc gtccccggaa tcgccgggcc gctgaagaac gcggtcacca
    34321 gctgggtcat ggtgacgcta tcgggcgtaa ccacgaagtt catcggcgtt ccggacgtgc
    34381 cgatcagcgg gctggtgaag gtgacgtcgc cggtcgtatt cacggatgcg ggcgaggtgg
    34441 tattcgcgtt gttcagcgtg atcgcgccac cgccggtgct cgccatcgta atgccgtttg
    34501 ccgtggtggc atccaggagg ttgatcgccc cgctgaccgt gacgttgccg cccaaaatgc
    34561 tcgcgcccgg cgcgtccgtc gtgatgttcg cgagcgccgt cgtatttccg atcgcgccca
    34621 tgaagttgtt ggtccccgag tcgttgaccg tcagcgaatt gccgccgttg atcgttccgc
    34681 cgaaggtcac gttgccggaa cccgtgctga tggtggtgtc gcttccgagg accgtgttac
    34741 cggtgatgcc aaatgcgccg ccactcgtgg cgtaggtgct gttcaacacc gcgttgccgt
    34801 tgtactgctg ggcgccggtc gtcgtgacgt ttccaagcga gatgctgggc gcagtgaccg
    34861 ccaggctcgc cagcggcgtt cctccgccca ccgcgccggc gaagctggcg ctggtcgccg
    34921 cgttgaccgc cagcgactgg gcaccgttga tcgtgctgga gaagccgatc gaaccggtgg
    34981 tcgtcgaggt cagcgtggtt ccagccccga gcagcactgc atccccatag ctctgcgcgc
    35041 cggtcgtgcg gatcaagccg ccgttgatgc tcgtcgagcc gccggcgttc gtggtgaagc
    35101 tcgcgagcgc cgtcgagttg ccaaccgcac ccgcgaacgt cgtgttgttc cccgagttga
    35161 cggtgagcgc attgccgccg ttgacggtcc ccgagaaggt catgtccccg gtgccgccga
    35221 cggttgtcgc tccggtcagc gtcacgttgc cgccgagcga agacgtgccg gtccaggcaa
    35281 gggcattgcc gccggcgcca gttcccgcca ccgataccgc ttctgcaccg attgcgacgc
    35341 cattgatatg gagcgtgccg ccgctcgcga tcgttgtccc gccgccggcg gtgccgagcg
    35401 cgcttgcatt cgaagcggtg agcgtgccgg cgttgatgct ggttacgccg gtgtaggtat
    35461 tggcgcccga caatgttgcc gtgcccgcag tgctcttggt cagtcccacg cccccggcaa
    35521 ggactgcgct gaccgtgccg ctttgaagcg cgaaatcggt tcccgaggcg agcgttccat
    35581 tctggagggt tcccgtcacc agggttaccg cattgaccgt gtcgctgaat ccgccgagct
    35641 cgaacgtgcc gccgttgact gacaggctgc tgccgtcggc tagcctgttg ctcgccccga
    35701 gggccagcgt tcccgcattc acggtcgtcg taccggtgta ggtgttggcg ccggaaagcg
    35761 tcatcgtgcc cgtgccgacc ttcgtgattc cgccggtggc gctggccacg ccgctgtaag
    35821 tcgtactggc gttgttgccg ccggtcgtca gcgtatttcc tcccgggagc gtcacatcgc
    35881 ccgcacccgc cagagaccca atggtttcgc tattgctcag ctggagcgtc gcgcccgaag
    35941 ccaccgtcac ggcgctcgcg tccgcgattg cagtaccacc ctgaacgatg agaaacccgg
    36001 cgtcgatggc tgtcgctccg gtgtacgcgt tggtcccgat gagcgtcatg ctgccggtgc
    36061 cttgctttgt aagtccgccg ggcccgctga tcgagcccga gtacgtcgtc gtgccgttgt
    36121 tccctccggt gctcagcgtg ttcccgccca gggtcacatt accgcccgcg gccccgccgc
    36181 cggccagcga tccgatcgtt tcgctcgccg ccaggtcgag cgttgcaccg gctgcgttcg
    36241 ccagcaccac ggcacccgtg tcgaggagcg ccgtaccgcc agccaggctc agcgtgccgc
    36301 cgtttacgtt ggtgatgccg gtgtaggtgt tggtgccgga cagagtcatc gtgccgctgc
    36361 ccactttcgt aagcgcgccg ccgcccgcgc cgctgatgac accactgaag gtcgtatcgc
    36421 tgttgtcggc gccggccgtc aataccgtcg tagccggcag cgttacactg cctgcgcccg
    36481 ccagcgagcc gatcgtttcg ctcgtggtca cgttgaacgt cgcgcctgcg gcgaccgtca
    36541 ccgcgctcgt atccgcaagg gctgtgccgc cctgcacggt cagcgtgccg gcagcgatcg
    36601 ccgtcgcgcc cgtatacgtg ttgctccccg atagtgtgaa cgtcccaacg ccctgcttgg
    36661 tgagaccgcc gctgccgctg atcatccccg cgtacgtggt cgtgccgtcg ttggctcccg
    36721 tggtcagcgt gttcgcgttg agcgtcacat ttccgccgag ggcgccgcca cccgccagcg
    36781 agccgatcgt ctcgcttgcc gcgaggttca gcgtcgcgcc ggccacgttg gcgagaacga
    36841 cggcgcccgc gtccaggatt gccgtgcccc cgtcgaggct cagcgtgccg ccgtttacgt
    36901 tggtcgtgcc ggtgtaggta ttggtgcccg agagtgtcag tgtgcctgcg ccgactttcg
    36961 tcaacccgcc cgtgccgctc accgcaccgc tgaaagtcgt gctggtgtcg tcggcacctg
    37021 cagtcagcgt ttgcgcgttg agggtcacgt cgccggcgcc ggccagcgag ccgatcgttt
    37081 cgttcgtacc caagttcagc gtcgcgccag cggccaccgt cacggcgccc gcgtcggcga
    37141 ttgccgcgcc gccgttgagc gtgatcctgc ccgcgttgat cgccgttgtg cccgtatggg
    37201 tgttggcccc cccgagcgtg accgtgctgg tgcccagctt ggtgagtccg cccgtgccgc
    37261 tgatcgtgct cgcgaccgtg gtatcggtcg catcgccgaa ggtaagcgtc gcatccagca
    37321 ccacgctcgt gcccccggcc cccgagagcc gcccgatggt ctcggccgca tcgatcgcca
    37381 gcgtcccgcc gttgagcgcc actactccgg catcggcgat cgccgccccg ccttgcagcg
    37441 tgaggttgcc cgcgctgatc gtcgtggtgc cggtgtaggt gttggtcccc gacagcgtca
    37501 gcgtgcccgc gccgtccttg gtgagcgtcc ccgtggtcgt ggcgatcgcc ccgctct
//
LOCUS       DATBSZ010000099         5543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_492468, whole genome shotgun sequence.
ACCESSION   DATBSZ010000099 DATBSZ010000000
VERSION     DATBSZ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492468"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1258
                     /locus_tag="VIG70_06480"
     CDS             41..1258
                     /locus_tag="VIG70_06480"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEY8250235.1"
                     /translation="MRLLYTIVLRLALPLILLRLWWRGRREPGYRDHVQERFGIYSGE
                     KPPKALWLHAVSVGEARAAAPLVRALKGLLPDHTVVMTCTTAAGRETLKQVYGQSVLA
                     AFLPYDYPESVQGFLERFRPRLGVLMETELWPNLLAQCERRAVPVVLANARMSEKSAR
                     GYRRWRVLTAPGIRSLAAVCAQSEADAERLRALGAPRVEVTGNLKFDATLDEAQLAAG
                     REWKAKLGRPVLLLASTREGEEKLLLDQFREEAFLVVVVPRHPQRFDEVAQWADARRS
                     RNDVPDGKTRIYLGDTMGEMAFYYAACDVAVIGGSFLPLGGQNLIEALAAGAPVVVGP
                     SMFNFAEATRLALDAGAAVQAADASTAIRQAVALISDRKKRDEMARAGKSLCEAHRGA
                     TAKHLEVCRRLIR"
     gene            complement(1215..2243)
                     /locus_tag="VIG70_06485"
     CDS             complement(1215..2243)
                     /locus_tag="VIG70_06485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY8250236.1"
                     /translation="MNSILVVRRDNIGDLVCTTPLFSALRRRYPRAWIGALVNSYNAA
                     VLDRNPDLDRVVAYTKLKHLDPGQSALGALARRVGDLWRLRRRRLDCVVLATPLVVPR
                     TFALARALAPARIVGFSDETLRPVAGLHEVERVFALAAHLGIDAPIPPLTVVPDPRLA
                     AQALDAFGLSREKFKVAVQISARRPAQQWPAERFAELVQRLDALGAAAMLLWSPGPAE
                     HPRHPGDDDKAAAVAERLAGKAPLVAYSTPRLPELIGALAACDAAITSDGGAMHLAAA
                     LQKPIVCFFGDVAPEQWRPWGVPHRVLRPQSRNVADISVDQTIAAFRELVAQASGSAA
                     GRLRDASR"
     gene            complement(2272..3624)
                     /locus_tag="VIG70_06490"
     CDS             complement(2272..3624)
                     /locus_tag="VIG70_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY8250237.1"
                     /translation="MPGPGRSTRLFRFTSGFVALALPLAAGAEDLMQVYREAQRSDAV
                     YAAARHSVDAGRERLPQGRALLLPTLNLSANAQRTRAEVDSRDPSISPSFIRYPESAS
                     YTLTFTQPLFRYQNWLQYEQGGHQVRQAEATFAQAYQDLIIRVAQAYTDVLAAHDTLA
                     LVRAQKAATAEQLAQAKRNFEVGTATITDTHEAQARYDLIAAQEIAALNDLESKRRAL
                     QLIIGREPGELATLRPDVKLNPPNPNDMQAWVELAEKQGYPVQIAEAAAEVAALEAKR
                     NRAAHLPTLDFVATHGQLGQNATTESQVGRDSTTTVIGLQFAMPLYQGGGLTSRDRES
                     AALSMKSKEDLDNARRNAALAARQNFLLVINGIAVVQALEQALISSQSALDSNKLGYE
                     VGVRINIDVLNAQQQLFLTRRDLAVARYGLIMANLRLKAAAGALREEDLERVNAVLVP
                     "
     gene            complement(3581..3904)
                     /locus_tag="VIG70_06495"
     CDS             complement(3581..3904)
                     /locus_tag="VIG70_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8250238.1"
                     /translation="MKQISPTELAAWLSDPRREKPVLLDVREPWEWQAARIEGAQHIP
                     MREVPARVEEIDRGQEVVAICHHGGRSQQVAMFLEKNGFAKIHNLVGGVDAWSRTVDP
                     AVPLY"
     gene            complement(3901..4554)
                     /locus_tag="VIG70_06500"
     CDS             complement(3901..4554)
                     /locus_tag="VIG70_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY8250239.1"
                     /translation="MNLEQARTNMVEQQIRTWEVLDQDVLDLLYVVPREEFVPAKHRT
                     LAFSDMEIPIGEGEKMWQPKMEARVIQELAIRKTDRVLEAGTGSGYLTALMSHRASRV
                     LSVELKASLAAFGRANLERHGADNVSLETGDAAHGWPSGAPYDVIVLTGSTPTLPRTL
                     AEQLAPGGRLFAVVGEPPVMSARLIVATAPGVWHGTDLFETVIAPLANAEHPARFRF"
     regulatory      4578..4686
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4677..>5543
                     /locus_tag="VIG70_06505"
     CDS             4677..>5543
                     /locus_tag="VIG70_06505"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEY8250240.1"
                     /translation="MNANPKFLAATAHVDAAAVQALPNSRKIHVGALRVPMREITQSG
                     GNPPIAVYDTSGPYTDPAAKIDIRKGLFPLRQDWIEARGDTQLLENPTSAYGRKRLED
                     PRLAELRFDLGRKPRRGKCVTQMHYARKGIITPEMEFIALRENQRLIDSLSVQHPGQS
                     FGAATPKEITPEFVRSEVARGRAIIPANVNHPETEPMIIGRNFLVKINCNIGNSAVTS
                     SIGEEVDKMTWAIRWGADTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKTGG
                     KAEDLTWEIFRDT"
BASE COUNT          875 a         1846 c         1945 g          877 t
ORIGIN      
        1 ggcagccgcc cgcggccggc gaagtgctgc aagcgctcgc atgagactgc tgtacacgat
       61 cgtgctgcgc ctcgcgctgc cgctcattct cctcaggctg tggtggcgcg ggcggcgcga
      121 gccgggctac cgcgaccacg tccaggaacg cttcgggatc tattcgggcg aaaagccgcc
      181 gaaggcgctg tggctgcatg cggtttcggt cggcgaggcg cgcgcggcgg cgccgctcgt
      241 gcgcgcgctc aaaggcctgc tgcccgatca caccgtagtc atgacctgca cgacggccgc
      301 ggggcgcgag acgttgaagc aggtgtacgg ccaatcggtg ctcgccgcct ttcttcccta
      361 cgactatccg gaatcggtgc agggcttcct cgagcgcttt cggccgcgcc tcggcgtgct
      421 gatggagacc gagctctggc cgaatctcct cgcgcaatgc gagcgccgcg ccgtacccgt
      481 ggtcctggcg aatgcccgca tgtcggagaa gtccgcgcgc ggctaccggc gctggcgcgt
      541 gctcacggcg cccgggatcc gaagccttgc cgcggtgtgc gcgcagagcg aagccgatgc
      601 ggagcgcctg cgcgcgctcg gcgcgccgcg ggtcgaggtc accggcaacc tcaagttcga
      661 cgccacgctc gatgaggccc agcttgcggc cggccgtgaa tggaaagcga agctcggccg
      721 gccggtcctg cttctcgcga gtacgcggga gggtgaagaa aaacttttac tggatcagtt
      781 ccgtgaggaa gcgttcctgg tcgtcgtggt tcctcgccat ccgcagcgtt tcgacgaagt
      841 cgcgcaatgg gccgacgccc gccgcagccg caacgacgtc ccggatggga agacccggat
      901 ctacctcggc gacacgatgg gcgagatggc gttctactac gctgcgtgcg acgtggcggt
      961 gatcggcggc agcttcctgc cgctcggcgg ccagaacctg atcgaggcgc tggccgccgg
     1021 cgcgccggtc gtcgtcgggc cgagcatgtt caacttcgcc gaggcgacgc ggcttgctct
     1081 ggatgccggg gcggccgtcc aggcggccga cgcttccacc gcgatacggc aggcagtggc
     1141 gctgatttca gaccggaaaa agcgcgacga aatggcgcgc gccggaaaga gcctctgcga
     1201 ggcgcaccgc ggcgctaccg cgaagcatct cgaagtctgc cggcggctga tccgctagcc
     1261 tgtgccacca gctcgcggaa tgccgcgatc gtctggtcga ccgagatgtc ggcgacgttg
     1321 cggctctgcg ggcgcagcac gcggtgcggc accccccacg ggcgccattg ctcgggcgcc
     1381 acgtcgccga agaagcacac gatcggcttc tgcagcgcgg ccgcgaggtg cattgcgccg
     1441 ccgtcgctgg tgatcgccgc gtcgcaggcg gcgagcgcgc cgatcagctc gggcagccgc
     1501 ggcgtactgt aggcgacgag cggcgccttt cccgccagcc gctcggcgac ggcggcggcc
     1561 ttgtcgtcgt ctccgggatg acgcggatgc tcggcggggc cgggcgacca cagcagcatc
     1621 gccgccgctc cgagcgcatc caggcgctgc accagctctg caaagcgttc ggccggccat
     1681 tgctgcgccg gccggcgcgc gctgatctgc acggcaactt tgaatttttc tctggaaaga
     1741 ccaaaagcat cgagcgcttg cgcggccaga cgcggatcgg gcacgaccgt cagcggcgga
     1801 atgggcgcgt cgatgccgag atgcgccgcg agcgcgaata cgcgctcgac ttcgtgcagg
     1861 ccggctaccg ggcgcagggt ttcgtcggag aagccgacga tgcgcgcggg cgcgagcgcg
     1921 cgtgcaagcg cgaacgtgcg cggcacgacg aggggcgtcg ccagcaccac gcagtcgagg
     1981 cggcggcgcc gcagccgcca aaggtcgccc acgcgccgcg cgagcgcgcc gagcgcggat
     2041 tggccggggt cgaggtgctt gagcttggta taggcgacga cgcggtccag gtccgggttg
     2101 cggtcgagca ccgccgcgtt atagctgttg acgagcgccc cgatccacgc tcgaggatag
     2161 cgccggcgca gggcgctgaa gagcggcgtg gtgcagacca ggtccccgat gttgtcgcgc
     2221 cgaacgacga ggatgctgtt caaaccgcga tgcagaacgc ggggttcgcg ttcagggcac
     2281 caggacggcg ttgactcgct cgaggtcttc ttcgcgcagc gctccagcgg cggccttgag
     2341 gcgcaggttg gccatgatga ggccgtagcg cgcgaccgcg aggtcgcgcc gcgtgaggaa
     2401 cagctgctgc tgcgcgttga ggacgtcgat gttgatgcgc acgccgacct cgtagccgag
     2461 cttgttcgaa tcgagcgcgc tttgcgagga gatgagcgcc tgctcgagcg cctgcaccac
     2521 cgcgatgccg ttgatcacca gcaggaaatt ctggcgcgcg gcgagcgccg cgttgcggcg
     2581 agcgttgtcg agatcttcct tcgacttcat gctgagggcg gcggattctc ggtcgcgcga
     2641 agtgagcccg ccgccctggt acagcggcat ggcgaactgc aggccgatga cggtagtggt
     2701 gctgtcgcgc cccacctggc tttcggtcgt ggcgttctgg ccgagctggc cgtgggtcgc
     2761 gacgaaatcg agcgtcggca ggtgcgcggc ccggttgcgc ttggcttcga gcgcggcgac
     2821 ctcggcggcc gcctcggcga tctgcaccgg atagccttgc ttctcggcga gctcgaccca
     2881 cgcctgcatg tcgttcgggt tgggcggatt gagcttgacg tccgggcgca gcgtcgcgag
     2941 ctcgccgggc tccctgccga tgatgagctg caacgcgcgg cgcttgcttt cgaggtcgtt
     3001 cagcgccgcg atctcctgcg cggcgatcag gtcgtagcgc gcctgcgcct cgtgggtgtc
     3061 ggtgatggtc gcggtgccga cctcgaagtt gcgcttcgcc tgcgcgagct gctcggcagt
     3121 cgccgccttc tgtgcgcgca ccagcgcgag cgtgtcgtgc gccgcgagga cgtcggtgta
     3181 cgcctgggcg acccggatga tcaggtcctg gtaggcctgg gcgaaggtgg cctcggcctg
     3241 ccggacctgg tggccgccct gctcgtattg cagccagttc tggtagcgaa acagcggctg
     3301 ggtgaaggtg agcgtatagc tcgccgactc cgggtagcgg atgaagctgg gcgagatcga
     3361 gggatcgcgc gaatcgacct cggcgcgcgt gcgctgcgcg ttggccgaga ggttcagcgt
     3421 cggcagcagc agcgcgcgtc cctgcggcag ccgctcgcgg cccgcgtcga ccgaatgccg
     3481 cgcggcggca tacacggcgt cgcttcgctg cgcctcgcga tagacctgca tcaggtcctc
     3541 ggcgccggcg gcgaggggaa gcgcgagagc gacgaagccg ctagtaaagc ggaacagccg
     3601 ggtcgaccgt ccgggaccag gcatcgacac cccccaccag gttatgaatt ttggcaaagc
     3661 cgttcttctc caggaacatc gccacctgct gactgcgccc gccgtggtgg cagatcgcga
     3721 ccacctcctg gccgcgatcg atttcctcca cccgcgccgg cacctcgcgc atcgggatgt
     3781 gctgcgcgcc ttcgatgcgc gcggcctgcc actcccaggg ctcgcgcacg tcgagcagca
     3841 ccggcttctc gcggcgcgga tcggagagcc acgcggcaag ctctgttgga gaaatctgtt
     3901 tcaaaatctg aatctcgcgg gatgctcggc gttcgcgagc ggcgcgatca cggtctcgaa
     3961 gagatcggtg ccgtgccaga cccctggcgc cgtcgccacg atcagccgcg cgctcattac
     4021 cggcggctca ccgaccacgg cgaacaggcg tccgcccggc gcgagctgct cggccagcgt
     4081 tcgcggcagc gtcggagtgg acccggtgag gacgatgacg tcgtagggcg ccccgctcgg
     4141 ccagccgtgc gccgcgtcgc cggtctcgag cgagacgttg tcggcgccat gccgctcgag
     4201 gttcgcgcgg ccgaacgccg ccagcgaggc cttcagctcg acggaaagca cgcgcgaggc
     4261 acggtgcgac atcagggcgg tcaggtagcc gctgccggtt ccggcctcca gcacccggtc
     4321 ggtcttgcgg atcgccagct cctggatcac ccgcgcctcc attttcggct gccacatctt
     4381 ttcgccttcc ccgatcggaa tttccatgtc ggagaacgcg agcgtgcggt gcttggcggg
     4441 gacgaactcc tcgcgcggca ccacgtacag gagatcgagc acgtcctgat cgagcacctc
     4501 ccaggtgcgg atctgctgct cgaccatatt ggtgcgtgcc tgctccagat tcatgggcac
     4561 gcggattata atcgtcgcct tagcgttggg ggtcccgggc ccaatggaag atgagggcct
     4621 gggtgagaga cacccttcga acctgatgcg ggtcatgccg ccgtagggag acgcaaatga
     4681 acgccaatcc gaagtttctt gccgccaccg cgcacgtcga cgccgccgcg gtccaggcgc
     4741 tgccgaattc caggaagatc cacgtcggcg cgctgcgcgt gccgatgcgc gagatcacgc
     4801 aaagcggcgg caatccgccg atcgcggttt acgacacgtc ggggccgtac accgatccgg
     4861 ccgcgaagat cgacatccgc aaggggcttt ttcctctccg gcaagactgg atcgaggcgc
     4921 gcggcgatac gcagctcctc gaaaacccga cctcggcgta cggcaggaag cgcctcgagg
     4981 atcccaggct cgccgagctg cgcttcgacc tggggcgcaa gccccgcaga ggcaagtgcg
     5041 tcacgcagat gcactacgcg aggaagggga tcatcacccc cgagatggag ttcatcgcgc
     5101 tgcgcgagaa ccaacggctg attgattcat taagtgttca gcatcccggg cagtcgttcg
     5161 gcgctgcgac tcccaaggag atcaccccgg aattcgtgcg cagcgaagtc gcccgcggcc
     5221 gcgccatcat tccggcgaac gtcaatcacc cggaaaccga gccgatgatc atcgggcgca
     5281 acttcctggt gaagatcaac tgcaacatcg gcaacagcgc ggtcacctcg tcgatcggcg
     5341 aggaggtcga caagatgacc tgggcgatcc gctggggcgc cgacacggtg atggacctct
     5401 ccaccggcaa gcacatccac gagacgcgcg agtggatcat ccgcaacagc cccgtgccca
     5461 tcggcacggt gcccatctac caggcgctgg aaaagaccgg cggcaaagcc gaggacctga
     5521 cctgggagat cttccgcgac acg
//
LOCUS       DATBSZ010000100         1992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2405686, whole genome shotgun sequence.
ACCESSION   DATBSZ010000100 DATBSZ010000000
VERSION     DATBSZ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405686"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /gene="pbpC"
                     /locus_tag="VIG70_06510"
     CDS             complement(<1..1410)
                     /gene="pbpC"
                     /locus_tag="VIG70_06510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEY8250241.1"
                     /translation="MGVGALWGLIDSFGPAPLGRDLEMSKTVLDRNGRLLRSYVTSQG
                     RWRLPATKEQVDPRFFDVLLAYEDKRFFQHHGVDPLAFGRAALQFITHGHIVSGGSTI
                     TMQVARLLEPRRERSLYVKLREAVRAIQLERVMSKDEILDLYLTLAPYGGNLEGIRAA
                     SLSYFGKEPRRLTLGEAALLVALPQSPEMRRPDRFHEAAKNARNRVLDRMARYGMFDA
                     AEIERAKIEPVPHVRKKMPMIAPHAADDAVAGAANAREIRLTIDGGLQKTLEGLALDR
                     ARALGPDMSVAIIVVDSATGEVLARVASPDYFDERRAGQVDLTRAVRSPGSALKPFIY
                     GIGFEDGLIHPETLIEDRPLRYGSYAPENFDLTFQGTVTVRRALQQSLNVPAVAVLEE
                     VGPSRLTARLAEAGAALVLPRREAPGLALGLGGVGIRLADLTMLYSGFARGGTVQPLV
                     EQLDAPRLGAKRLLEPVAAW"
     gene            complement(1599..>1992)
                     /locus_tag="VIG70_06515"
     CDS             complement(1599..>1992)
                     /locus_tag="VIG70_06515"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250242.1"
                     /translation="DVTHAKQNQRFVVVLKITDAQPQFARVALIDYLPAGFEIDNPRL
                     VSSGDTGTLDWIENAGTPVHTEFRDDRFTAAFDRTNSDPAVFTVAYVVRAVSPGDYVL
                     PQAGVEDMYRPDRFGRTGTGKVGVQAAK"
BASE COUNT          328 a          647 c          695 g          322 t
ORIGIN      
        1 ccaggcggcg acaggttcca acaggcgctt cgcgccgagc cttggagcat caagctgctc
       61 gaccagaggc tgcaccgtgc cgccgcgcgc aaagcccgaa tagagcatgg tgaggtctgc
      121 gagcctgatg ccgacaccgc cgaggccgag ggccaatcca ggcgcctcgc gtcgcggcag
      181 cacgagcgcg gcacctgcct cggcaagacg cgcggtgagc cggctcgggc caacctcttc
      241 gagcaccgcg accgccggca cgttcagcga ctgctgcaac gcgcgccgca cggtaacggt
      301 accctggaag gtgaggtcga aattttccgg ggcatagctg ccgtagcgga gcggccggtc
      361 ctcgatcagc gtttcgggat ggatcagccc atcctcgaag ccgattccgt agatgaaagg
      421 cttgagcgcc gatcccggcg agcgaacggc gcgggtgagg tcgacctggc cggcgcgccg
      481 ctcgtcgaaa tagtctggcg atgcgacgcg ggcgagcacc tcgccggttg cgctgtcgac
      541 cactatgatg gcgaccgaca tgtcgggccc gagcgcccgg gcccggtcga gcgcgagccc
      601 ttccagagtc ttctgtagtc cgccgtcgat ggtgaggcgg atctcgcgcg cgttcgccgc
      661 gccggccacg gcgtcgtcgg cggcgtgcgg cgcgatcatc ggcatcttct tgcgtacatg
      721 cggcaccggc tcgatcttgg cgcgctcgat ttccgccgca tcgaacatcc cgtaacgcgc
      781 catgcggtcg agcacccggt ttcgcgcgtt cttggccgct tcgtggaagc gatcaggccg
      841 gcgcatctcc ggcgattgcg gcagggccac gagcagggct gcttcgccga gcgtgagccg
      901 gcgcggctcc ttgccgaaat aagagagcga ggcggccctg atcccttcca gattgccgcc
      961 gtaaggagcg agcgtgaggt agaggtcgag gatctcgtcc ttgctcataa cgcgctcaag
     1021 ctgaatggcg cggactgctt ccctgagctt cacgtaaagc gagcgctcgc gtcggggctc
     1081 gagcaggcgt gcgacttgca tggtgatggt cgagccgccg gagacgatat gcccgtgggt
     1141 gatgaattgc agcgcggcgc ggccgaaggc gagcggatcg acgccgtgat gctggaagaa
     1201 gcgcttgtcc tcataggcca agagcacgtc gaagaagcga ggatcgactt gctctttagt
     1261 cgccggcagg cgccagcggc cctggctcgt gacgtaggac cgaagcagcc gcccgttgcg
     1321 gtcgagaacg gtcttcgaca tttcgaggtc gcgcccgagc ggcgccggac cgaacgagtc
     1381 gatcaatccc cacagcgcgc cgacccccac gcagactaac gcggcaagcc cgacagctgc
     1441 ataacggatg cggcgccacc actttcccct ctcccctgtg gggagagggc tggggtgctg
     1501 gggctccgag cggcgaggag aaatcttgtt cccctcaccc ggcgtgccga gcgcgccgtc
     1561 gtctccgcgc cggggagagg aggacgtgga tcgctggttc atttggcggc ctgcacgccg
     1621 accttgccgg tgccggtacg gccgaagcgg tctgggcggt acatgtcctc gaccccagcc
     1681 tgcggcagca catagtctcc cggcgatacc gcccgcacca cgtaggccac ggtgaacacc
     1741 gccggatcgc tgttggtgcg gtcgaaggcc gcggtgaagc ggtcgtcgcg gaactcggta
     1801 tgcaccggcg tgccggcatt ctcgatccag tcgagcgtgc cggtgtcgcc ggaggagaca
     1861 agccgcggat tgtcgatctc gaagcctgcc ggcagatagt cgatcagcgc cacgcgcgcg
     1921 aattgcggtt gcgcgtcggt gatcttgagc accaccacga agcgctggtt ctgcttggcg
     1981 tgggtgacgt cg
//
LOCUS       DATBSZ010000101         2640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_151151, whole genome shotgun sequence.
ACCESSION   DATBSZ010000101 DATBSZ010000000
VERSION     DATBSZ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151151"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1150
                     /locus_tag="VIG70_06520"
     CDS             200..1150
                     /locus_tag="VIG70_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEY8250243.1"
                     /translation="MGRKKLLGAALVVALAAAAASVLMPGGEQAKPGTEAQVSARPGA
                     EAGARAEPIDLPERSSLRKPGGELFAAPPPPPAPARPVVEARPEPKPQAPPMPYRVAG
                     QVSHEGTTVVLARDERVYLVRVGETLDGGYRVESIRPDGVTLVYTPLDLREHLAAAST
                     LRVDPPAALAQAAKPAPVAAAKAIAPAQLRWQGPARVQAGNPFEVALKLTSDQAVRGS
                     PLQLSYDARLLEPVAVRAGEFFADGSFTYRVSPDGSILVGAFGKGEVPADAEFLVVTF
                     KPVRAGVTAELKLSSVALQDAAGGPVVHAPLAAFRTSITP"
     gene            1147..1530
                     /locus_tag="VIG70_06525"
     CDS             1147..1530
                     /locus_tag="VIG70_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250244.1"
                     /translation="MSCKRLLALALALSALLLVPQLAGAHGDHAQPPSIAQEEAAPSV
                     APAAVSVAPSLSESCPGAPGRKCCCGTAHALAGAGKITSVNANAWTLAPAVAASATKF
                     ADSSVPPIAAPWSSSARPRAPPLSA"
     gene            1614..>2640
                     /locus_tag="VIG70_06530"
     CDS             1614..>2640
                     /locus_tag="VIG70_06530"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY8250245.1"
                     /translation="MQSVSRFAAPAALLLAAATSTAALAQERPISLDKPSAAAKALGD
                     LEISGFAVGSINYSSHIQMVPEFAGGAQALSDPRQLNFRFDKVGLALAKRFGPTLSVS
                     AAFEIESHKDKHSHLRTNPTTQCVGVPVPCESFGAETPTTESTLDKLAITWFPWTSLG
                     FSFGRFDVPFGIERHDEVLNMTATTSEIYHFAKPQNYTGLQTMWQLGPVVDVNLWIAN
                     RWESHTTHEPFDDNNSAKSFGGRIGFSPARRDELLNFGIGGWYGAERDNPANVADLGM
                     NGPKRTLIDFDATWSPSSQAAYVAEVVWGTEEDVSFRARGWPISQPAQNNIKPKWFGG
                     SVMGHWEL"
BASE COUNT          399 a          965 c          889 g          387 t
ORIGIN      
        1 gcgggcgcgg acggcgccgc ggacatcatc aagtgccagc gcaagccacc aaggcagcga
       61 gattacgcgg cggcgttccc cccgcgcagt gggcacggct gcaggcggtg ttcccggctg
      121 gatcgtgcga ttcgtcacgc ggggttgcca tcgactaagc aggattgcgc gccccgcggg
      181 gggcgcataa gctaggtctg tgggaagaaa gaaactcctc ggcgcagcac tcgtcgtcgc
      241 cctcgcggct gcggccgcaa gcgttttgat gcccggcggc gagcaagcga agccgggcac
      301 tgaagcgcaa gtgtccgctc gccccggcgc cgaagcgggt gcgcgcgcgg agccgatcga
      361 cctgcccgag cgctcgagcc tgcgcaagcc gggcggcgag cttttcgccg cgccgcctcc
      421 gccgcccgcc cccgcgcggc cggtcgtcga ggcgcggccg gagcccaagc cccaggcacc
      481 ccccatgccc taccgcgtcg cggggcaggt gtcgcacgaa ggaaccacgg tggtgctcgc
      541 gcgcgatgag cgcgtgtatc tcgttcgagt gggagaaacg ctcgacggcg gctaccgcgt
      601 cgagtcgatc cggcccgacg gcgtaacgct cgtgtacacg cccctcgacc tgcgcgagca
      661 tctcgccgcc gcttcgacgc tgcgcgtcga tccgccggcc gcgcttgcgc aggcggcgaa
      721 gcccgcgccg gtcgccgccg cgaaagccat cgcgccggcg cagctgcgct ggcagggacc
      781 ggcgcgcgtg caggccggca atccgttcga ggtcgcgctc aagctcactt ccgaccaggc
      841 cgtgcgcggc tcgccgctgc agctctcgta cgacgcgcgc cttctcgagc cggtcgcggt
      901 gcgcgccggc gagtttttcg ccgacggcag cttcacctac cgcgtgagcc ccgacggctc
      961 gatcctggtc ggcgccttcg gcaagggcga ggtgccggcg gacgccgaat tcctggtggt
     1021 gacgttcaag ccggtgcgcg ccggcgtcac cgccgagctc aagctctcct ccgtggcgct
     1081 gcaggacgcg gccggcgggc ccgtggtcca cgcgccgctc gcggctttcc gcacctcgat
     1141 taccccatga gctgcaagcg tctgctggcg cttgcgctcg ctctgagtgc tcttctacta
     1201 gtaccgcagc tggccggcgc gcacggcgat cacgcgcagc cgccgtcgat cgcgcaggaa
     1261 gaagcggcgc cctccgtcgc gcccgccgcg gtttccgtcg cgccaagcct gtcggaatca
     1321 tgcccgggcg ctcccgggcg caagtgctgc tgcggcaccg cgcacgcgct cgccggcgcc
     1381 ggcaagatca cgtccgtcaa tgccaatgcc tggacgcttg cgcccgcggt ggccgcgagc
     1441 gcaacgaagt tcgccgattc ttcagtcccc ccgatcgccg cgccgtggag ctcctcggcg
     1501 cgcccccgcg cacctccgct ttccgcctga ttcacccgta gtccgtggcg ccgcgccggg
     1561 gaaagcccgc gcggcgtgcg cttcgtacgt caaaacggaa aggggaggaa gccatgcaat
     1621 cggtatcgag gtttgccgcg cccgcggcgt tgctgctcgc cgcggcaacc agcaccgcag
     1681 ctctcgcgca ggaacgtccg atttcgctcg acaagccgtc cgcggccgca aaggctctcg
     1741 gcgatctcga gatctcgggc tttgccgtcg gctcgatcaa ttacagctcg cacatccaga
     1801 tggtcccgga gtttgccggc ggcgcgcagg cgctttccga cccgcgccag ctgaatttcc
     1861 gcttcgacaa ggtgggcctc gcgctcgcca agcgcttcgg gccgacgctc agcgtcagcg
     1921 cggcgttcga gatcgagagc cacaaggaca agcacagcca cctgcgcacc aacccgacca
     1981 cgcagtgcgt gggcgtgccg gtcccctgcg agagcttcgg cgccgaaacc ccgacgaccg
     2041 agtccacgct cgacaagctg gcgatcacct ggtttccctg gacctcgctc ggcttttcct
     2101 tcggccgctt cgacgtgccc ttcggcatcg agcggcacga cgaagtgctg aacatgaccg
     2161 ccacgacgag cgagatctac cacttcgcca agccgcagaa ttacaccggg ctgcagacga
     2221 tgtggcagct cgggccggtg gtcgacgtca acctgtggat cgccaaccgc tgggagtcgc
     2281 acaccacgca cgagccgttc gacgacaaca actcggcgaa gagcttcggc gggcgcatcg
     2341 gcttctcgcc ggcgaggcgc gacgagctgc tcaacttcgg catcggcggc tggtacggcg
     2401 ccgagcgcga caacccggcc aacgtcgcgg acctgggcat gaacggcccg aagcgcacgc
     2461 tgatcgactt cgacgcgacc tggtcgccga gctcgcaagc cgcgtacgtc gccgaagtgg
     2521 tatggggtac ggaagaggac gtctccttcc gcgcgcgcgg ctggccgatc tcgcagcccg
     2581 cgcagaacaa catcaagccc aagtggttcg gcggctcggt catggggcac tgggagctga
//
LOCUS       DATBSZ010000102        23619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_14894, whole genome shotgun sequence.
ACCESSION   DATBSZ010000102 DATBSZ010000000
VERSION     DATBSZ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23619
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14894"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..522)
                     /locus_tag="VIG70_06535"
     CDS             complement(106..522)
                     /locus_tag="VIG70_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8250246.1"
                     /translation="MNFQRGKQKEAPEINLVPMIDVMLVILIFLMITTTYSKYTELQI
                     NLPSAQAERQLERPNEVTILVNAQGQYVVNRTPVPFRSVEQLAGELRRAAAALQEPIV
                     VISADAAATHQSVIRVMEAARVAGLSQITFTTQSNK"
     gene            complement(519..1130)
                     /locus_tag="VIG70_06540"
     CDS             complement(519..1130)
                     /locus_tag="VIG70_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY8250247.1"
                     /translation="MFAIVQAAGWPIWFLLLASVIAVALIIERSVSLRASKIIPPQLL
                     DQVVGVYKRQGLSPEVIERLSRDSPLGAVLAAGLRNLKSSRYVMKEAIEEAGRGVAHD
                     LERFLTTLGTIATAAPLLGLFGTVIGMIEIFGSQSPTGGTNPQQLAHGISIALYNTAF
                     GIAIAIPALIFYRHFRARVDTFVVEMEQLAAKLVDIVHGERAA"
     gene            1561..2730
                     /gene="xseA"
                     /locus_tag="VIG70_06545"
     CDS             1561..2730
                     /gene="xseA"
                     /locus_tag="VIG70_06545"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY8250248.1"
                     /translation="MAELLRSVRDTLERRFPLAWVRGELSNLSRAPSGHCYFTLKDGA
                     AQVDCVMFRSRTAALDWEPRDGMQVEVRALPTLYEPRGRFQLTVEGMRRAGLGPLHER
                     FLRLKEKLGAEGLFDPALKREPPAFPRRIGVVTSLAAAALRDVLTTLERRNPAIEVIV
                     YPVPVQGESAAERIAAMLARASRRAECDVLLLVRGGGSIEDLWPFNEEAVARAIRASR
                     IPVVAGIGHETDFTIADFAADRRAPTPTAAAELVSPARAELAARLIRGIRCLSREMER
                     RFRYAAQAVDSCARRLVHPAERLRSYQSLVTQLKARLAFSFTHKVHRCSTHVERLQAS
                     LASLDPTAVLARGYSITYNAAGEVMKDSKYVQTGERIRTALARGSIESQVKKTVD"
     gene            2736..3287
                     /locus_tag="VIG70_06550"
     CDS             2736..3287
                     /locus_tag="VIG70_06550"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY8250249.1"
                     /translation="MGHRLSKIATRTGDAGETGLGDGTRVPKDHPRVAALGDIDELNS
                     ALGLVLAEEVPADIRAALGEVQQDLLDLGGELSIPGHSLLKPERVAFLDESLASWNEG
                     LEPLKEFILPGGSRAAAAAHLARTVCRRAERRVVALARKDPVSEPARQYLNRLSDLLF
                     VTGRVLNRAAGVGDVQWRHQRKR"
     gene            3263..3814
                     /locus_tag="VIG70_06555"
     CDS             3263..3814
                     /locus_tag="VIG70_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8250250.1"
                     /translation="MAPPAQALEGLLARSALGDRGAFRALYDATAPKLFGVALRILRR
                     EDWAEEVLQECYVSVWRHATEYSASRAAPMTWMTSIVRNRCLDWLRRPNPQPLDDEQL
                     EALESDNPGPLAELERGRDTAALARCLRGLDAKQRQAIALAFFDGLSHAELASHLREP
                     LGTVKTWVRRGLARLRNCLELQG"
     gene            3798..4481
                     /locus_tag="VIG70_06560"
     CDS             3798..4481
                     /locus_tag="VIG70_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY8250251.1"
                     /translation="MNYRDKPELRERLAAEYALGTLRGRARERLRRWLRDDAELAREV
                     AAWEARLVPMAEAIPPVAPPPRLWQGIENRLENARKPFSFWKTLGLVASGAAAALLAV
                     AVLLPPQRAGISPSYIAVLSDPKTNHPVLVATAERGEMVLRVNTLDPAIHVAGRSLEL
                     WALPRGAQPRSLGLVSDQKAILRLAAAADQSLGDVPALAVSLEPQGGSPTGQPTGPVL
                     YMGPCVKYW"
     gene            4481..4885
                     /locus_tag="VIG70_06565"
     CDS             4481..4885
                     /locus_tag="VIG70_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8250252.1"
                     /translation="MPREETATRYRVLIVDDEESMRLVLAGMLSQELPVDVQLAGTSE
                     QALRLAEKQPFDAILLDLMMPGVGGFGVLYGVRRSVPNATTPVIMVSVFSEKKTIERC
                     LAAGANAYLVKPVRSAELAAAVKKQLATRRRA"
     gene            4878..5261
                     /locus_tag="VIG70_06570"
     CDS             4878..5261
                     /locus_tag="VIG70_06570"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8250253.1"
                     /translation="MPEIKIYNVDALGKPVGPYSHVARVKGGETLYIAGMLAPGDSFE
                     AQCSAVYAQIEKALQSAGGGWKNVVQFTTYLVHSQDIARFYRWREQNYPKMFPDGKYP
                     PNTLLIIDRLVQEQFLIEIQTVAAI"
     gene            complement(5286..6794)
                     /locus_tag="VIG70_06575"
     CDS             complement(5286..6794)
                     /locus_tag="VIG70_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY8250254.1"
                     /translation="MEVLNNLILGFGVAISPINLLYALIGVMLGTLIGVLPGIGPVAT
                     IAMLLPITFNLNPIAALIMLAGIYYGAQYGGSTTAILVNLPGESASVVTCLDGYQMAR
                     QGRAGPALAVAACGSFFAGCVATLIIAVAAPPLAEVALKFGPAEYFSLMVLGLVAATV
                     LAHGSLIKAIAMVVFGLLLGIIGTDVNSGVLRFTFDVPELSDGIGFVVVAMGMFGTAE
                     IIANLEQGEHREVFTSKVGRLMPTREDIRRAIPAVLRGTALGSFLGILPGGGALLSSF
                     GAYTVEKKLSKHPEQFGKGAIEGVAGPEAANNAGAQTSFIPMLTLGIPGNAVMALMIG
                     ALMIQGIAPGPQVMNEKPQLFWGLIASMWVGNAFLVILNLPLIGMWIKLLTVPYRYLY
                     PAILVFMSIGVFSLSNNPFDVLIMAIFGVLGYICVKLECEPAPMILGFILGPLMEENL
                     RRAMLLSRGDPTTFVTKPISAGFIIASVILLVIVALPAIRKKRDEAFVEQKT"
     gene            complement(6808..7425)
                     /locus_tag="VIG70_06580"
     CDS             complement(6808..7425)
                     /locus_tag="VIG70_06580"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY8250255.1"
                     /translation="MKTKIKGPKDFWAGLMFIGVGVFFLIWALAHYQMGTAVRMGPAY
                     FPAMLGGLLAFLGVLVLLESFVMRGGELKLPFNIVDMVVALAIFAAVMYLGKLLAFSM
                     DFAILGATAIVSVLTIRYRPNAKALVLISAACVVYGYLMKPLGLVLATAALVFIGAYG
                     GHEFKWKEVTILFLVLIVFSILVFVKGLTLPFPVCPAFMETCPIR"
     gene            7491..8081
                     /locus_tag="VIG70_06585"
     CDS             7491..8081
                     /locus_tag="VIG70_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY8250256.1"
                     /translation="MRWAVVLVLIFGCSQRPKLERLPSDAVVLAFGDSLTFGTGANED
                     ESYPAQLEALIGRRVVRAGVPGEVTAQALARLPAALDEHAPRLLLLCIGGNDFLRRLG
                     NQQAERNVREMVQLARSRGISVLLIGTPEPGFTVSPPAFYAGIAKEFRLPYEGGIIGQ
                     VLKDASLKADPIHPNARGYRLIAERVAETLKSSGAI"
     gene            complement(8032..8625)
                     /locus_tag="VIG70_06590"
     CDS             complement(8032..8625)
                     /locus_tag="VIG70_06590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEY8250257.1"
                     /translation="MIIVFARAPVPGEAKTRLIPKLGAWRTARLHERLTLHALRTARL
                     ASGRVELHGTRRHSFFRRLGVAFRLQRGRGLGERMVHALSKARRPAILIGTDCPVLTS
                     ADLRRAARWLRGGCDAVLAPAEDGGYALIGLRRARKEFFEGILWGGPQVYADTVKRLA
                     GYRWRALRRVWDLDRPEDLERFRSLRLTSGFRRPALR"
     gene            complement(8622..9293)
                     /locus_tag="VIG70_06595"
     CDS             complement(8622..9293)
                     /locus_tag="VIG70_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEY8250258.1"
                     /translation="MVAQRLSVVIPALNEARGIRAALEALAPLRARGHEVIVADGGSV
                     DGTVELAAGLCDRVLNAPRGRAVQMNAGAHAATGFALVFLHADTRLPAQADDLVLESL
                     KKHLWGRFDAKIDGRHPLLKIVGCAMSLRSRLTGIATGDQAIFVRREAFPGFPEIALM
                     EDVAFCKRMKRGGPPACLRAKVLTSGRRWESRGVLRTIVLMWSLRLLYFLGAPPERLI
                     RFYKQ"
     gene            complement(9254..10009)
                     /locus_tag="VIG70_06600"
     CDS             complement(9254..10009)
                     /locus_tag="VIG70_06600"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEY8250259.1"
                     /translation="MRWVFLVVLALAPPAPAFDHSHPGWNALLKKHVVVLDGGKASRL
                     NYAAIAQERAALQGYLEGLSRVGEAEFQGWSRPEQMGFLINAYNAHAVEKVLTRYPNL
                     KSIWDFGRFFANPFRDPFFSLFGARMSLDTIEHEILRKKYPDPRIHYAVNCASIGCPM
                     LREEAYAGAHLEKQLEEQAERFLSDRTRNRLRDGRLEVSKIFDWFKEDFEPRDKYFQR
                     YAGVLGLPPGASPPLVFLDYDWSLNDSRSSSPR"
     gene            complement(10006..12132)
                     /locus_tag="VIG70_06605"
     CDS             complement(10006..12132)
                     /locus_tag="VIG70_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8250260.1"
                     /translation="MSRARLLVVGVFILAAVVFFAAGGQRYFSFENLKAQQAALEAWR
                     DAYPWQSGLVFFAVYVAFTSLSLPAASLLTLLAGAIFGLGWGVLIVSFASAVGATLAM
                     LAARFVLRDWVQERFRAQLQGVNRGVEKEGAFYLFTLRLIPAVPFFLINLAMGLTPIR
                     VWTYYWVSQVAMFPATVLYVNAGTQLARLDSLRGIFSWQLIGAFVLLGLFPLAAKRVV
                     DFVSARRVYARWRKPRRFDRNLVVVGAGSAGLVAAYIAAAVRAKVTLVEKHRMGGDCL
                     NTGCVPSKALIKTAKVLSQIRRAKDFGLKSATAEFDFAEVMERVQRVIRTIEPHDSVE
                     RYTQLGVECIKGDARITTPWTVEVNGKTLTTRAIVIAAGAEPFIPPIPGIDTVECLTS
                     DTVWGLRKLPPRLLVLGGGPIGSELAQAFARLGSKVTQVEMFERILPREDPEFSHMVA
                     ARMREDGVDVRVGHKAVRFTGTNLLLCEHQGREVRIEFDALLCAVGRVARTKGYGLEE
                     LGIPVTKARTVETNEFLQTAYPNIYACGDVAGPYQFTHTAAHQAWYASVNALFGGLKK
                     FRADYSVIPWATFTDPEVARVGLNETEAKERAIPYEVSVYGIDDLDRAIADEAAHGKV
                     KVLTVPGKDRILGATIAGEHAGDLILEFVAAMRHGFGLNKILGTIHIYPTFGEANKYA
                     AGVWKKAHAPQGLLARLEKLHTWMRG"
     gene            complement(12138..12680)
                     /locus_tag="VIG70_06610"
     CDS             complement(12138..12680)
                     /locus_tag="VIG70_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8250261.1"
                     /translation="MPERPQQLSHLEYLGDATKFAGQMHALIPKCALLENFSAAEVQL
                     LAHFMDVYRAEATREIIREGDGGDFLLMVLEGTVEVHKRDRWNTQQLLASVDAGRTLG
                     EMSMIDGEPRFATCIAAAPSVIAVLHRESLARIIVEQPLLGAKILMELVLMLSQRLRA
                     TSQRLLGLLDEQSQGAAGIL"
     gene            complement(12715..13800)
                     /locus_tag="VIG70_06615"
     CDS             complement(12715..13800)
                     /locus_tag="VIG70_06615"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY8250262.1"
                     /translation="MTEPINDKTSLTDDERIDNIVPLPPPEHLIRFFPIQGTAVESQI
                     AETRARLRQILHGRSDRLLVVIGPCSIHDPAAALEYARKLSGERKRLSGELEILMRVY
                     FEKPRTTVGWKGLINDPYLNGSFRINEGLRIARDLLVRINYAGVPAGCEFLDVISPQY
                     IGDLVSWGAIGARTTESQVHRELASGLSAPVGFKNGTDGNVRIAVDALLAARQKHHFL
                     SVHKSGQVAIVETRGNDDCHIILRGGKAPNYDAAAVQAACTELARAKLPERLMIDCSH
                     ANAAKDYRRQAAVAADIAGQIAGGERRIVGVMVESHLVEGRQDLEPGRALTFGQSITD
                     ACLGWDDSVRLLEEMAHAVAERRKRQR"
     gene            complement(13977..14393)
                     /locus_tag="VIG70_06620"
     CDS             complement(13977..14393)
                     /locus_tag="VIG70_06620"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY8250263.1"
                     /translation="MTTTTHRDFKSFGDGYLPGLVGVEIVTVSRELVESRLAVRREVM
                     APNGFLHAASVIALADTSCGYGCVAALPEGAKGFTTVELKSNFLGTARDGTIACRATP
                     VHLGRTTQVWDAVITHEATGAKIALFRCTQMVLWPK"
     gene            14526..15479
                     /locus_tag="VIG70_06625"
     CDS             14526..15479
                     /locus_tag="VIG70_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8250264.1"
                     /translation="MAIPIAGFKDVYDVSSVEKALQELSPTANEALRALYEKMMRLGG
                     QRFTVKPSALPEMERLFGELPNFTEVLEDIRKHLALCIDSNDSVELPPMLLLGDPGIG
                     KTHFARKVAALLGTGFGFVPMSSLTAGWVLSGASSQWKNAKPGKVFDTFLNGDYANPV
                     IVVDELDKASGDGQYDPLGALYELLEVQTATRFIDEFVELPIDASGAVWLATANDAAR
                     IPEPLLDRLAVYEIEAPDAEGSARIASTIYQEIRGSHDWGRQFPEAPAASLLERLASL
                     PPRDMRRALHAAFGTAKLAGRTELGPDDIQDGRSKKQRIGF"
     gene            complement(15480..15983)
                     /locus_tag="VIG70_06630"
     CDS             complement(15480..15983)
                     /locus_tag="VIG70_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HEY8250265.1"
                     /translation="MSPAPGGALGALEASGLGQAMRQWLWLYPSVETIHIVGIGLLFG
                     SIAVLDVRLLGFSRHMSARTLAKHVLPWTLCSFLLIIPSGLLMFTAHATEFIDSEVFI
                     LKMGLIMAAALNAALFHTITFRTADVWDSEEMRKLPPPPSARLAGALSLLLWISVIAC
                     GRLLAYF"
     gene            complement(15980..16339)
                     /locus_tag="VIG70_06635"
     CDS             complement(15980..16339)
                     /locus_tag="VIG70_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEY8250266.1"
                     /translation="MKRLFVAGLFALPLAVSAHHGWSEYDTSNALKLTGKIVESGYEH
                     PHGHIRFEAKGEGATAGKTWTVTLAPPSRMENRGLPKEWLKAGAAATVEGYPNRNKPE
                     EMRAERITINGKTIELR"
     gene            complement(16336..18981)
                     /gene="alaS"
                     /locus_tag="VIG70_06640"
     CDS             complement(16336..18981)
                     /gene="alaS"
                     /locus_tag="VIG70_06640"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY8250267.1"
                     /translation="MKSNAIRNGFLEYFRSKGHTVVPSSSLVPANDPTLLFVNSGMVQ
                     FKDVFLGQDKRPYKRATTAQRSLRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     KDAIQFAWELLTRVYKLQPDRLWATVYETDDEAYELWTKMIGLPAGRVVRIGDKAGGQ
                     KYQSDNFWQMADTGPCGPCSEIFYDHGPGIEGGPPGSPEADGDRYIEVWNLVFMQFNR
                     DEKGVMHPLPKPSVDTGMGLERIAAVLQGKHSNYEIDLFQDLIKAAHRETIGSTKQGD
                     LKNPSLNVIADHIRACSFLIVDGVIPGNEGRGYVLRRIVRRAIRHGYKLGQKQPFFFR
                     MVDDLDRAMGDAYPELRKAKGRVAEVLKQEEERFAETLENGMTVLESALASGEKLLDG
                     ETVFRLYDTFGFPVDLTADIARERGVRIDMAGFEAAMDQQRERARAASKFSAASTLEY
                     QGGKTEFHGYDTLALPAKVVALYREGAQAQALKSGDSGTVVLDRTPFYAEAGGQVGDR
                     GELVGTGGTFEVADTQKIQADVYGHQGTLKTGTLKVGDKVEARVNTTLRASTMRNHSV
                     THLMHKALREVLGSHVQQKGSLVDADKTRFDFSHDKPVTDQQIREVELRVNTEIMQNA
                     PTRAQVMPIEEAKKSGAMMLFGEKYGDEVRVLEIGSSREFCGGTHVGRTGDIGVFKIT
                     SEGGVAAGIRRVEATTGANALALMNRQLEEFHEVARELRAQPGAGMVEKAVKSLLDEK
                     KALEKELARARSKLAMGQGTDLASQAVDVKGKDGAATRVLVATLDGADAKTLRETMDK
                     LKDRLKSAAIVLGAVNDGKVALIAGVTDDLTGKLKAGELVNYVAQQVGGKGGGRPDMA
                     QAGGTEPAKLPVALQSVKSWVEQRV"
     gene            complement(19042..19380)
                     /locus_tag="VIG70_06645"
     CDS             complement(19042..19380)
                     /locus_tag="VIG70_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250268.1"
                     /translation="MDQAQALAIVRSLANGVDPESGEVFAADSSYQRPQVVRALYEAA
                     AALERIERFERRRSQMPPKTGEPWSEDEDRKLLAAFDAGRALQELAAAHERTMGAVRA
                     RLLKYGRINA"
     gene            19545..20432
                     /locus_tag="VIG70_06650"
     CDS             19545..20432
                     /locus_tag="VIG70_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250269.1"
                     /translation="MLAPAAALAQAVTDAELQKRANAILTLMGFAVTPDVTTGSLSLT
                     DQTAGNPFFRQTSLSGGGTLPGRRLYLEGTLAYGRYDPDFATNDDSPASRVAVKWDSL
                     LGTGGVGWDFPLAAHLVLRPIFNFSLGRIESEGMVTAAPPATRSPALEFLSNGRLNAY
                     GLGGSLMLDYERYRPGNEIDVELRYSSIHLQSFDSSRAVEGEADAQTLSLWSRYRAPT
                     GMTALERPVRYVLEYAYTRFLGDLDGTLGFTYVNSFGGGLELDSSARDVYVTRSRLMV
                     RYKVGNNVTGWAVGLGLSF"
     gene            complement(20456..21001)
                     /gene="recX"
                     /locus_tag="VIG70_06655"
     CDS             complement(20456..21001)
                     /gene="recX"
                     /locus_tag="VIG70_06655"
                     /inference="COORDINATES: protein motif:HMM:NF001055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEY8250270.1"
                     /translation="MQSLATSRRAMGSGFLSGMPAAGKSSSPRGSAAQLEPDSLSELR
                     ARALRHLARREHSRDELARKLSPYAESPEVLEGLLRELESKKQLSDERFAEVRTHWLA
                     RKYGAAKIRQDLTSKGIPQELAERAAVGLNETEKAKAILERKYRAPAATREERAKRAR
                     FLQGRGFSGDVIRRLVLQSED"
     gene            complement(20895..21203)
                     /locus_tag="VIG70_06660"
     CDS             complement(20895..21203)
                     /locus_tag="VIG70_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250271.1"
                     /translation="METWYCKTLGDGRAAYAPSLRIQQMFSPLFAASGQPSSMAVFSR
                     HDRKANKVTAYFSPRAAKLAALFDAKPCDKPPSDGIGLLVGDARSWEIFFPERKRRAA
                     "
     gene            complement(21207..22271)
                     /gene="recA"
                     /locus_tag="VIG70_06665"
     CDS             complement(21207..22271)
                     /gene="recA"
                     /locus_tag="VIG70_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY8250272.1"
                     /translation="MDDNKSKALAAALSQIEKQYGKGSIMRMDDAAIKDIDVVSTGSL
                     GLDLALGVGGLPRGRVVEIYGPESSGKTTLTLQVIAEMQKIGGTAAFIDAENALDISY
                     AAKLGVKTADLLISQPDTGEQALEIADMLVRSGAVDVIVIDSVAALTPKAEIEGEMGE
                     PQMGLQARLMSQALRKLTANIKRTNTLVIFINQIRMKIGVLFGNPETTTGGNALKFYA
                     SVRMDIRRIGSIKKGDEVIGSETRVKVVKNKVAPPFKQAEFDILYGEGISREGEVLET
                     GVNLAVLEKSGAWYIYNGDRLGQGKDNARDFLKENPALAREIEVKIREKAGVQKAAPL
                     AAAAEEADSKVEKLPTRKRG"
     gene            22417..23295
                     /locus_tag="VIG70_06670"
     CDS             22417..23295
                     /locus_tag="VIG70_06670"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8250273.1"
                     /translation="MAKVAFLGLGVMGYPMAGHLLKKGGHDVTVYNRSPAKAQQWAKE
                     YGGKHAATPREAAKDCDFVMMCVGNDDDVRSVAYGDNGVLAGMKKGAILVDHTTASAT
                     VARELSQKTKEKGIGFVDAPVSGGQAGAVNGQLGIMCGGEQATFDKAKPVLDTYAKMC
                     ALIGGPGAGQLTKMVNQITIVGMAQALAEGLAFAKRNNLDLEKVISVISKGAAQSWQM
                     ENRWKPMSELKTEGFGFAIEWMRKDVGICLAQAKQSNARLPVTALVDQFYAALEARGG
                     KRWDSTASLIQLLLKD"
     gene            complement(23292..>23619)
                     /locus_tag="VIG70_06675"
     CDS             complement(23292..>23619)
                     /locus_tag="VIG70_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250274.1"
                     /translation="SQADIAGNTIRMNGGNGIQVDRNSQAEIGSNTIDQNTGNAVNAA
                     FGAGVNLGVGTTARWQLRANASTVANTRFALGCTTGGYTTGSLGGLLGTLGTKSILNG
                     CIDTVP"
BASE COUNT         3967 a         7984 c         7763 g         3905 t
ORIGIN      
        1 ccgaatcttg tagagaagaa gccggagcgc gacgacgatg ccaaacagca ggctcagcgg
       61 ccacagcagc aaggctactg ggcctcgtcg gtaccagaac ttcacctact tgttcgactg
      121 ggtggtaaag gtgatctgcg acaggccggc gacgcgcgcg gcctccatca cgcggatcac
      181 cgactggtgc gtggcggcgg cgtcggcgct gatgacgacg atcggctcct gcagcgcggc
      241 ggcggcgcgg cgcagctcgc cggcgagctg ctcgacgctg cgaaacggca ccggggtacg
      301 gttgaccacg tactgcccct gcgcgttcac caggatcgtc acctcgttcg gtcgctcgag
      361 ctggcgctcg gcctgcgcgc tcggcaggtt gatctgcagc tcggtgtact tcgagtaggt
      421 ggtggtgatc atgaggaaga tcaggatcac cagcatcacg tcgatcatcg gcacgaggtt
      481 gatctcgggc gcctccttct gcttgccgcg ctggaagttc acgccgcgcg ctcgccgtgc
      541 acgatgtcga ccagcttcgc cgcgagctgc tccatctcga cgacgaaggt gtccaccctc
      601 gctctgaagt gccggtagaa gatgagcgcc gggatcgcga tcgcgatgcc gaaggcggtg
      661 ttgtagagcg cgatcgagat gccgtgcgcg agctgctgcg ggttggtgcc gccggtcggc
      721 gactgcgagc cgaagatctc gatcatgccg atcaccgtgc cgaacaggcc gagcagcggc
      781 gcggcggtgg cgatggtgcc gagggtggtc aggaagcgct cgaggtcgtg cgccacgccc
      841 cgtcccgcct cctcgatcgc ctccttcatc acgtagcgcg agctcttcag gttgcgcagg
      901 cccgccgcca gcacggcgcc gagcggcgag tccctggaca ggcgctcgat cacctcgggc
      961 gacaggccct ggcgcttgta caccccgacg acctggtcaa gcagctgcgg cgggatgatc
     1021 ttgcttgccc gcagcgagac cgagcgctcg atgatcaggg caacggcgat gacggaagcg
     1081 aggaggagga accagatggg ccagcctgcg gcctggacga tcgcgaacaa cgcgtttcct
     1141 tatcgttatt ttggttgcta cggctactga ctgtaagcca aagggtcggc gcgcagcaag
     1201 ttccgcagcg cagcaaatcc gccgcccgtg gcaccgcagg agcgtcgcgc cagcgcaacg
     1261 cgtagtgcaa tttgcgatat ccacaaaaac ctgtggataa gtttgtggat tgtcgatgta
     1321 cggactgtca caagcccgtt gcgggcccgg taaagacggt ggcgatcaaa atttgagcag
     1381 gatttttcgc aagcccgaca acccgttacg caaaagaatc ggggtccgcg agcgcttctg
     1441 tcgccgcccg gcggcgctcg gccgcgaatg tggattagtg taggctcgct cgcactcttg
     1501 acaccgatgc agaacgatat tcccgggccc gctgtcgttt ccgccttacg catcctgtcg
     1561 gtggcggagt tgctgcgcag cgtgcgcgac acgctcgagc ggcgctttcc gctcgcctgg
     1621 gtgcgcgggg agctctccaa cctctcgcgc gcgccctcgg gccactgcta tttcactctg
     1681 aaggacggtg ccgcgcaggt cgattgcgtc atgttccgca gccgcacggc ggccctcgac
     1741 tgggagccgc gcgacggcat gcaggtcgag gtgcgcgccc tgccgacgct gtacgagccg
     1801 cgcgggcgct ttcagctcac cgtggaaggc atgcgccgtg ccggcctcgg gccgctccac
     1861 gagcgcttcc tgcggctgaa ggaaaagctc ggcgccgagg gcctgtttga tccggctttg
     1921 aaacgcgaac caccggcatt tccccgcagg ataggggtcg taacgtcttt ggcggcagca
     1981 gcgctgcgcg acgttctgac caccctagag cgccgcaacc ccgcaatcga agtgatcgtc
     2041 tatccggtcc cggtccaggg cgagagcgcg gcggagcgca tagcggcgat gctcgcccgc
     2101 gcgagccgcc gcgccgagtg cgacgtactg ctgctcgtgc gcggcggcgg ctcgatcgag
     2161 gacctttggc cgttcaacga agaggccgtc gcgcgcgcca tccgagcgtc gcgcatcccg
     2221 gtcgtagcgg gcatcggcca cgaaaccgac ttcaccatcg ccgatttcgc cgccgaccgg
     2281 cgcgcgccga ccccgacggc cgccgccgag ctcgtcagcc ccgcgcgcgc ggagctcgcc
     2341 gccaggctga tccgcggcat tcgctgcctg agccgcgaga tggagcggag atttcgctac
     2401 gcggcgcagg ccgtcgattc ctgcgcgcgg cgcctggtgc atccagccga gcggttgcgc
     2461 tcgtatcaga gcctcgtgac ccagctcaaa gcgcgcctgg ccttttcctt cactcacaaa
     2521 gtgcaccgct gctccaccca tgtggagcgg ctgcaggcca gccttgcgag cctggatcca
     2581 accgcggtgc tcgcgcgcgg ctacagcatc acctacaacg cggccggaga agtcatgaag
     2641 gattcgaaat acgtgcaaac aggcgagcgc atcaggaccg cgctggcgcg aggcagcatc
     2701 gagagccaag tgaagaaaac cgtggactga accctatggg ccatcggcta tcgaaaatcg
     2761 ccacccgcac cggcgacgcc ggcgagaccg gcctgggcga cggcacccgg gtgccgaaag
     2821 accacccgcg cgtagccgcg ctcggcgaca tcgacgagtt gaacagcgcc ctgggactcg
     2881 tcctcgccga ggaggtgccg gccgacatcc gcgccgcgct cggcgaggtg cagcaggacc
     2941 tgctggatct cggcggcgag ctgagtattc cgggccactc gctcctgaaa cccgagcggg
     3001 tcgcgtttct cgacgagtcc ctggcctcgt ggaacgaggg gctggaaccc ctcaaggaat
     3061 tcatcctgcc cggcggcagc cgcgcggcgg cggcggcgca tctcgcgcgc accgtgtgcc
     3121 ggcgcgccga gcgccgcgtg gtcgccctcg cacgcaagga ccctgtaagc gagcccgcgc
     3181 gccaatacct taaccggctt tccgatctgc tgttcgtaac cggccgcgtg ctcaacaggg
     3241 cggccggggt gggcgacgta cagtggcgcc accagcgcaa gcgctagaag gcctccttgc
     3301 ccgcagcgcg ctgggcgacc gcggcgcgtt ccgcgcactt tatgacgcga cggcaccgaa
     3361 actgttcggg gtcgccctgc gtatattgag acgtgaagac tgggccgagg aagtgctgca
     3421 ggaatgctac gtcagcgtgt ggcgccacgc gacggagtac agcgcgagcc gcgccgcgcc
     3481 gatgacctgg atgacgagca tcgtgcgcaa ccgctgcctc gactggctgc gccggcccaa
     3541 cccgcagccg ctcgacgacg agcagctcga agcgctggag agcgataacc cggggccgct
     3601 ggccgagctc gagcgcggcc gcgataccgc cgccctcgcc cgctgcctgc gcggcctgga
     3661 cgccaagcag cgccaggcga tcgccctcgc gttcttcgac ggcctgtcgc acgccgagct
     3721 cgcgagccac ctgcgcgagc cgctcggcac ggtgaagacc tgggtgcgcc gcgggctcgc
     3781 ccgcctgagg aattgccttg aactacaggg ataagcccga gctgcgcgag cgcctcgccg
     3841 ccgagtacgc gctcggcacg ctgcgcggcc gcgcgcgcga gcggctgcga cgctggctgc
     3901 gcgacgacgc cgagctggcg cgcgaggtag cggcctggga ggcgcgcctt gtgccgatgg
     3961 ccgaggccat cccccccgtg gcgccgccgc cacgcctgtg gcaagggatt gaaaatcgac
     4021 tggagaacgc aagaaagccg ttctcgttct ggaaaaccct cggcctcgtc gcgagcggcg
     4081 ccgcggcggc gctgctcgcg gtggccgtgc tgctgccgcc gcagcgcgcg gggatctccc
     4141 cttcgtacat cgcggtgctg agcgatccga agaccaatca tccggtgctg gtcgcgaccg
     4201 cggagcgggg cgaaatggtg ctgcgcgtca ataccctcga cccggcgatc cacgtcgcag
     4261 gccgcagcct cgagctgtgg gcgctgcctc gcggcgcgca gccgcggtcg ttgggattgg
     4321 tttctgacca aaaagccata cttaggctgg ccgcggcagc cgaccagtcg ctcggcgacg
     4381 ttccggcgct ggcggtgagc ctcgagccgc aaggcggctc acccaccgga cagccgaccg
     4441 ggccggtgct ctacatgggg ccttgtgtaa agtactggtg atgccacgcg aggaaacggc
     4501 gacccgctac cgggtactga tcgtcgacga cgaggaatcg atgcggctcg tgctggcggg
     4561 gatgctctcg caggagctgc cggtcgacgt gcagcttgcc ggcaccagcg agcaggcgct
     4621 gcgcctcgcc gagaagcagc cctttgacgc catcctcctc gacctgatga tgcccggcgt
     4681 cggcggcttc ggcgtgctct acggcgtgcg ccgctcggtg ccgaacgcca ccacgccggt
     4741 catcatggtg tcggtgttct ccgagaagaa gaccatcgag cgctgcctgg cggccggcgc
     4801 caacgcctac ctcgtcaagc cggtgaggtc cgccgagctc gcagccgccg tgaagaagca
     4861 gctcgccacg cgccgccgtg cctgagatca agatctacaa cgtcgacgcc ctcggcaagc
     4921 ccgtcggccc ctactcccac gtcgcgcgcg tcaagggcgg cgagacgctc tacatcgccg
     4981 gcatgctcgc gccaggcgac agcttcgagg cgcaatgctc ggccgtctat gcgcagatcg
     5041 agaaggcgct gcagtccgcg gggggcggct ggaagaacgt ggtgcagttc accacgtatc
     5101 tcgttcattc gcaggacatc gccaggttct accgctggcg ggagcagaac tacccgaaaa
     5161 tgttccccga cggaaagtac ccgccgaaca cgctgctcat catcgaccgc ctcgtccagg
     5221 agcaattcct gatcgagatc cagaccgtcg ccgcgatcta agaagcgcca gaagccgcgc
     5281 taacttcagg tcttctgttc gacgaaggcc tcgtcgcgct ttttgcggat ggccggcagc
     5341 gccacgatca ccagcaggat caccgacgcg atgatgaagc cggcgctgat cggcttggtg
     5401 acgaaggtcg tgggatcgcc gcgcgagagc agcatcgcgc gccgcaggtt ctcctccatc
     5461 agcgggccga ggatgaagcc gagaatcatc ggcgccggct cgcactcgag cttgacgcag
     5521 atgtagccca gcacgccgaa gatcgccatg atcagcacgt cgaacgggtt gttcgacagg
     5581 ctgaagacgc cgatcgacat gaataccagg atcgcggggt agaggtagcg gtagggcacg
     5641 gtcaggagct tgatccacat gccgatcagc ggcaggttga ggatcacgag gaacgcgttg
     5701 ccgacccaca tcgaggcgat cagcccccag aagagctgcg gcttctcgtt catcacctgc
     5761 ggtccgggcg cgatgccctg gatcatcagc gcgccgatca tcagcgccat caccgcgttg
     5821 cccgggatgc cgagcgtgag catcgggata aaggaagtct gcgcgccggc gttgttggcg
     5881 gcttcggggc cggcgacgcc ctcgatcgcg cctttgccga actgctcggg gtgcttggag
     5941 agcttcttct cgaccgtata cgcgccgaac gaggagagca gcgcgccgcc gccgggcagg
     6001 atgccgagga acgagccgag cgcggtgccg cgcagcaccg cggggatggc gcgccggatg
     6061 tcttcgcgcg tcggcatcag gcgcccgacc ttcgaggtga agacttcgcg gtgctcgccc
     6121 tgctcgaggt tggcgatgat ctcggcggtg ccgaacatgc ccatcgccac cactacgaag
     6181 ccgatgccgt cggagagctc cggcacgtcg aaggtgaagc gcagcacgcc ggaattgacg
     6241 tcggtgccga taatcccgag cagcagcccg aacaccacca tggcgatcgc cttgatgagc
     6301 gagccgtgcg cgagcaccgt ggccgcgacg agacccagca ccatcagcga gaagtactcc
     6361 gccgggccaa atttgagcgc cacttcggcg agcggcggcg cggcgacggc gatgatcagc
     6421 gtggcgacgc agccggcgaa gaacgagccg cacgccgcga cggcgagcgc cggcccggcg
     6481 cgcccctggc gcgccatctg gtagccgtcc aggcaggtga ccaccgaagc cgattcgccc
     6541 ggcaggttga cgagaatcgc ggtggtggag ccgccgtact gcgcgccgta gtagatcccg
     6601 gcgagcatga tcagcgccgc gatcgggttc aggttgaagg tgatcggcag cagcatggcg
     6661 atggtcgcca ccgggccgat gccgggcagc acgccgatca gcgtgccgag catcacgccg
     6721 atcagggcgt agagcaggtt gatcggcgag atcgcgacgc cgaacccgag gatcaggttg
     6781 ttgaggactt ccatcatcgc cgtcccccta gcggatcggg caggtttcca tgaaggccgg
     6841 gcacaccggg aacggcagcg tcaggccttt cacgaacacc aggatcgaga acacgatcag
     6901 cacgaggaac aggatcgtca cttccttcca cttgaactcg tgaccgccgt acgcgccgat
     6961 gaacaccagc gcggcggtgg cgagcaccag gccgagaggc ttcatcaggt agccgtacac
     7021 cacgcaggcg gcgctgatca gcaccagcgc cttggcgttc ggccggtacc ggatcgtcag
     7081 caccgacacg atcgcggtcg cgccgagaat cgcgaagtcc atcgagaagg cgagcagctt
     7141 gccgaggtac atcacggcgg cgaagatggc gagcgcgacc accatatcga cgatattgaa
     7201 aggcagcttg agctcgcccc cgcgcatcac gaacgattcc agcaggacga gcacaccgag
     7261 gaacgcgagc aggccgccga gcatggcggg gaagtacgcc ggccccatgc gcaccgcggt
     7321 tcccatctgg taatgggcca gcgcccagat cagaaagaac acgccgacgc cgatgaacat
     7381 cagcccggcc cagaagtcct ttggaccctt gatcttggtc ttcatcccct ctcctccgcc
     7441 gctttcgcgg tctgcggcaa agtgtagcat ttcgagccga aatgcaagcc ttgcgctggg
     7501 ccgtcgtcct cgtgctcatt ttcggctgca gccagcgccc caaactggag cgcctgccga
     7561 gcgacgcggt ggtgctcgcg ttcggcgaca gccttacctt cggcaccggc gcgaacgagg
     7621 acgagagcta tccggcgcag ctcgaagcct tgatcggccg gcgcgtggtg cgcgccggcg
     7681 tgcccgggga ggtcacggcg caggcgctcg cccgcctgcc cgccgcgctc gacgagcacg
     7741 cgccgcggct cctgctgctc tgcatcggcg gcaacgactt cctgcggcgg ctcggcaacc
     7801 agcaggcgga acgcaatgta cgagagatgg tgcagctcgc gcgtagccgc ggcatctcgg
     7861 tgctactcat cggcaccccc gagccgggct tcaccgtgtc gccgccggcg ttctacgccg
     7921 gcattgcgaa ggaattccgc ctgccctacg aaggagggat catcggccag gtgctgaagg
     7981 acgcgagcct caaggccgat ccgatccacc ccaacgcacg cggctatcgc ctcatcgcag
     8041 agcgggtcgc cgaaaccctg aagtcaagcg gagcgatctg aagcgctcca ggtcctcggg
     8101 ccggtcgagg tcccagaccc ggcgcagcgc gcgccagcga tagccggcca gtcgcttcac
     8161 ggtgtccgca tagacctgcg ggccgcccca gaggatgccc tcaaagaatt ccttccgggc
     8221 gcggcgcagg ccgatcagcg cgtaaccccc atcctccgcc ggggcgagca cggcgtcgca
     8281 gccgccgcgc agccagcgcg ccgcgcggcg caggtccgcg gaggtcagaa ccgggcaatc
     8341 ggtgccgatg aggatcgccg ggcgtcgtgc tttggaaagc gcgtgcacca tgcgctcgcc
     8401 gaggccgcgg ccgcgctgca ggcggaaggc gacgccgagg cgcctgaaaa acgaatgccg
     8461 gcgcgtgccg tgaagctcca ctcgcccgct tgcaagcctg gcggtgcgca gggcatgcag
     8521 cgtcaggcgc tcatgcagcc gcgccgtgcg ccaggcgccg agcttcggga tgaggcgcgt
     8581 cttcgcctct cccggcaccg gtgcgcgcgc gaacacgatg atcattgctt gtagaaacga
     8641 atcaaacgct ccggaggcgc accgaggaaa tagagcagcc gcaagctcca catcaggacg
     8701 atcgtgcgca gcacgccgcg cgactcccag cgccgccccg aggtgagcac cttcgcgcgc
     8761 aggcaggcgg gcggcccgcc gcgcttcatg cgcttgcaga aggcgacgtc ttccatcagc
     8821 gcgatctcgg ggaagccggg aaaagcctcg cgccgcacga agatcgcctg gtcgccggtg
     8881 gcgatgcccg tcagccgcga gcgcaggctc atggcgcagc cgacgatttt cagcagcgga
     8941 tggcggccgt cgatctttgc atcgaacctg ccccagaggt gctttttcaa tgactccagc
     9001 accagatcat cggcctgggc agggagcctc gtatcggcat gcaggaacac aagcgcaaac
     9061 ccggtggcgg cgtgcgctcc ggcgttcatc tgcacggctc ggccgcgcgg tgcgttcaat
     9121 acccggtcgc acaggcccgc cgcgagctcg accgtgccgt cgacgctacc gccgtcggcg
     9181 acgatcacct cgtggccgcg agcgcgcagc ggggcaagcg cctcgagcgc ggcgcggatg
     9241 ccgcgcgcct cgttcagcgc ggggatgacg accgagagtc gttgagcgac caatcgtagt
     9301 ccaggaagac gagcggcggg ctggcgcccg gcggcaaacc caatacgcct gcatagcgct
     9361 ggaaatattt gtcgcgcggc tcgaagtcct ccttgaacca gtcgaagatc ttcgacactt
     9421 cgagccggcc gtcgcgcagc cggtttcgcg tgcggtcgga cagaaagcgc tccgcctgtt
     9481 cctccagttg cttttccaaa tgcgctccag cgtacgcctc ctcgcgcagc atcgggcagc
     9541 cgatcgaggc gcagttgacc gcgtagtgga tgcggggatc gggatatttc ttcctcagga
     9601 tctcgtgctc gatcgtatcc aggctcatgc gcgcgccgaa cagcgagaag aacgggtcgc
     9661 ggaacggatt ggcgaagaac cggccgaaat cccagatcga cttgaggttc gggtagcggg
     9721 tgagcacctt ctcgaccgcg tgcgcgttgt aggcgttgat gaggaagccc atctgctcgg
     9781 gccgcgacca gccctggaac tcggcctcgc ctacgcgcga cagcccttcc aggtatccct
     9841 gcaatgcggc gcgctcctgg gcaatggcgg cgtagttcag ccgcgaggcc ttgccgccgt
     9901 cgagaaccac gacgtgcttc ttgagcagcg cattccagcc cgggtgcgaa tggtcaaacg
     9961 ccggcgccgg cggtgcgagc gcaagcacga cgaggaaaac ccacctcacc cccgcatcca
    10021 cgtgtgcagc ttttccagcc gggcgagcag gccctgcggc gcatgcgcct tcttccagac
    10081 accggcggcg tacttgttcg cctcgccgaa agtcggatag atgtggatcg tgccgaggat
    10141 cttgttcagg ccgaagccgt ggcgcatcgc ggcgacgaac tcgagaatca ggtcgccggc
    10201 gtgctcgccg gcgatggtag cgccgaggat gcggtccttg cccggcacgg tgagcacctt
    10261 gaccttgccg tgcgcggcct cgtcggcgat cgcgcggtcg agatcgtcga tgccgtacac
    10321 cgacacctcg tagggaatgg cgcgctcttt cgcctcggtc tcgttcagcc cgacgcgcgc
    10381 cacctcggga tcggtgaacg tagcccacgg gatcaccgag tagtcggcgc ggaatttctt
    10441 cagcccgccg aagagcgcgt tgaccgacgc gtaccacgcc tggtgcgcgg cggtatgggt
    10501 gaactggtag gggccggcga cgtcgccgca ggcgtagatg ttcggatagg ccgtctgcag
    10561 gaactcgttg gtctccaccg tgcgcgcctt cgtcaccggg atgccgagct cctccagccc
    10621 ataacccttg gttcgcgcga cacggcccac ggcgcagagc agcgcgtcga actcgatgcg
    10681 cacctcgcgg ccctggtgct cgcagagcag gagattcgtg ccggtgaacc gaacggcctt
    10741 gtggccgaca cgcacgtcca cgccgtcttc gcgcatgcgc gcggcgacca tgtgcgagaa
    10801 ctccgggtcc tcgcgcggca ggatgcgctc gaacatctcg acctgcgtca ccttcgagcc
    10861 caggcgcgcg aacgcctgcg cgagctccga gccgatcggc ccgccgccca gcaccaggag
    10921 ccgcggcggc agcttgcgca gcccccagac cgtgtcggag gtgaggcact ccaccgtgtc
    10981 gattcccggg atcggcggga tgaaaggctc cgcgcccgcg gcgatcacga tggcgcgggt
    11041 cgtgagcgtc ttgccgttga cttccacggt ccacggcgtg gtgatcctcg cgtcgccctt
    11101 gatgcactcc acgccgagct gcgtgtagcg ctccactgaa tcgtgcggct cgatcgtgcg
    11161 aatgacgcgc tgcacgcgct ccatcacctc ggcaaagtcg aactccgccg ttgcgctttt
    11221 caagccaaag tccttggctc ttcttatctg agacaagacc ttggccgtct tgatgagcgc
    11281 cttggaagga acgcagccgg tgttcaggca gtccccgccc atgcggtgct tctccaccag
    11341 cgtgaccttg gcacgcacgg cggccgcgat ataggcggcg accaggccgg cggagccggc
    11401 gccgacgacc accaggttgc ggtcgaacct gcgcggcttc ctccagcgcg cgtagacccg
    11461 gcgcgcgctt acgaaatcca cgaccctctt cgccgccagc ggaaacaagc cgagcagcac
    11521 gaacgcgccg atcagctgcc aggagaagat gccccgcagc gagtcaaggc gcgcgagctg
    11581 cgtgcctgcg ttcacgtaga gcaccgtggc cgggaacatc gccacctggc tcacccagta
    11641 gtacgtccat acgcggatcg gcgtcagccc catcgcgaga ttgatgagga agaacggcac
    11701 cgccggaatg agacgcagcg tgaaaaggta gaacgcgcct tccttctcca cgccgcggtt
    11761 cacgccctgg agctgcgcgc gaaaacgctc ctgcacccag tcgcgcagca cgaagcgcgc
    11821 ggcgagcatc gcgagggtgg cgccgaccgc cgaggcgaaa gacactatca gcacccccca
    11881 gcccaggccg aagatcgccc cggcgaggag cgtgagcagc gacgccgccg ggagcgaaag
    11941 cgaagtaaac gccacgtaga cggcgaaaaa gacgagcccg ctctgccacg gatacgcgtc
    12001 gcgccaggcc tcgagcgcgg cctgctgcgc cttgaggttc tcgaagctga agtagcgctg
    12061 gccgccggcg gcgaaaaaga cgacggcggc gaggatgaaa acgccgacaa cgagaagccg
    12121 ggctcggctc aagccgccta caaaatcccc gccgcgcctt gggactgctc gtcgagcagc
    12181 ccgaggagcc gctggctggt ggcgcgcagc cgctgcgaca gcatgagcac gagctccatc
    12241 aggatcttgg cgccgagcag cggctgctcg acgatgatgc gcgccaggct ctcgcgatgg
    12301 agcaccgcga tcaccgaggg cgcggcggcg atgcaagtgg cgaagcgcgg ctcgccgtcg
    12361 atcatcgaca tctcgccgag cgtgcgcccg gcgtcgaccg aggcgagaag ctgctgggtg
    12421 ttccagcggt cgcgcttgtg cacttcgacc gtgccttcga gcaccatcag caagaagtcg
    12481 ccaccgtcgc cctcgcggat gatctccctg gtcgcctcgg cgcggtagac gtccatgaag
    12541 tgcgcgagga gctgcacctc ggccgcggag aagttctcca gcagcgcgca tttcgggatc
    12601 agcgcatgca tttgcccggc gaacttggtg gcgtcgccga ggtactcgag gtgcgaaagc
    12661 tgttgcgggc gttcgggcat gagcgggcgg ctcgcgcccg attatgccga aaccctaccg
    12721 ctggcgctta cgccgctcgg ccacggcgtg ggccatttcc tccagcagtc ttaccgaatc
    12781 gtcccagccg aggcaggcat ctgtaatact ttgtccgaac gtgagcgccc ggcccggctc
    12841 gaggtcctgc cgtccctcga ccaggtgcga ttccaccatc acgccgacga tgcgccgctc
    12901 gccgccggcg atctgcccgg cgatgtcggc ggcgaccgcg gcctggcggc gatagtcctt
    12961 cgcggcgttc gcgtgcgagc agtcgatcat caggcgctcg ggcagcttgg cgcgggcgag
    13021 ctcggtgcac gccgcctgca ccgccgcggc gtcgtagttc ggcgcctttc cgccgcgcag
    13081 gatgatgtgg caatcgtcgt tgccgcgcgt ctccacgatc gccacctgcc cgctcttgtg
    13141 caccgagagg aagtggtgtt tctggcgcgc tgcgagcagc gcgtcgaccg cgatcctgac
    13201 gttgccgtcg gtgccgttct tgaacccgac cggcgccgac agccccgagg cgagctcgcg
    13261 gtgcacctgg ctctcggtgg tgcgcgcgcc gatcgcgccc cacgacacca ggtcgccgat
    13321 gtactgcggc gagatgacgt ccagaaactc gcagcccgcc ggcacgccgg cgtaattgat
    13381 gcgcaccagg agatcgcgcg cgatgcgcag gccctcgttg atgcggaagc tcccgttgag
    13441 gtacgggtcg ttgatcagcc ccttccagcc caccgtggtg cgcggcttct cgaagtacac
    13501 gcgcatcagg atctcgagct cgccgctcag ccgtttcctc tctccactca attttcttgc
    13561 atattccaaa gcggcagccg ggtcgtggat cgagcacggc ccgatcacga ccagcagccg
    13621 gtcggagcgg ccgtgcagga tctggcgcag ccgggcgcgc gtctcggcga tctgcgattc
    13681 gacggcggtc ccctggatcg ggaagaagcg gatcaggtgc tccggcggcg gcagcggaac
    13741 gatgttgtcg atgcgctcgt cgtcggtcag tgatgtcttg tcgttgatgg gctcggtcat
    13801 gaacgcctcg ggcaaaaaaa aaccgccagg ctttgggcac tggcggtttt tggttggatt
    13861 cggtggtgag tgctagccgg tcccttccgc cagttggcag gggaagtaaa agaagaagcc
    13921 gaagaagaac cggtgcatca tcgagcggga ttcaaacatg aaagcgcatg cactgtctac
    13981 ttgggccaca acaccatctg cgtgcaccgg aaaagcgcga tcttcgcgcc ggtggcctca
    14041 tgggtgatca cggcgtccca gacctgggtg gtgcggccca ggtgcaccgg ggtcgcccgg
    14101 caagcgatgg tgccgtcgcg cgcggtgccg agaaagttgc tcttcagctc caccgtggtg
    14161 aatcctttcg cgccttcggg cagcgcggcg acgcagccgt agccgcagct cgtgtcggcg
    14221 agggcaatca ccgacgccgc atggaggaag ccgttcggcg ccatcacctc gcggcgcacc
    14281 gccaaacggc tctccaccag ctcgcgcgac accgtgacaa tttccacacc caccaggccg
    14341 ggaaggtagc cgtcgccgaa acttttgaag tcccgatgcg ttgttgtagt catggatcga
    14401 ctgtacgtga ttggcactct acacagaggg ctgccaggca aggcgtttgc taggcggaaa
    14461 gcatcatgct agcgttttcc caccggcaag cgatcaagaa aggcaggcga taaatgtcgg
    14521 ctaacgtggc tatccccatc gcgggcttca aggacgtcta cgacgtctcg tcggtcgaga
    14581 aagcgctgca ggagctttcg cccaccgcga acgaagcgct gcgcgcgctc tacgagaaga
    14641 tgatgcgcct cggcgggcag cgcttcaccg tcaagccctc ggcgctgccg gagatggagc
    14701 gcctgttcgg cgagctgccg aacttcaccg aggtgctgga agacatccgc aagcatctcg
    14761 ccctgtgcat cgactcgaac gactcggtcg agctgccgcc gatgctgctg ctcggcgacc
    14821 ccggcatcgg caagacgcac ttcgcgcgca aggtggccgc cctgctcggc accgggttcg
    14881 gcttcgtgcc gatgagctcg ctcaccgccg gctgggtgct gtcgggcgct tcgtcgcagt
    14941 ggaagaacgc caagccgggg aaggtgttcg atactttcct gaacggcgac tacgccaacc
    15001 cggtgatcgt ggtcgacgag ctggacaagg cgagcggcga cggccagtac gacccgctcg
    15061 gcgcgctcta cgagctgctc gaggtgcaga ccgcgacgcg cttcatcgac gagttcgtcg
    15121 agctgccgat cgacgcctcg ggcgcggtgt ggctcgccac cgccaacgac gcggcgcgca
    15181 tccccgagcc gctgctcgac cgcctcgcgg tgtacgagat cgaggccccc gacgccgagg
    15241 gctcggcgcg catcgcgtcc accatctacc aggagatccg cggctcgcac gactgggggc
    15301 gccagttccc cgaggcgccc gcggcgtcgc tgctggaaag gctcgcctcg ctgccgccgc
    15361 gcgacatgcg ccgcgcgctg cacgccgcgt tcggcactgc caagctcgcg ggacgcaccg
    15421 agctcggccc ggacgacatc caggacggac gctcgaagaa gcagcggatc ggcttttagc
    15481 tagaagtacg cgagcaaccg cccgcaggcg attaccgaga tccagagcag cagcgacagc
    15541 gccccggcga ggcgcgccga cggcggcggc gggagcttgc gcatctcctc cgagtcccac
    15601 acgtccgcag tgcggaaggt gatggtgtgg aagagcgccg cgttcagcgc ggcggccatg
    15661 atcaggccca tcttgaggat gaagacctcg ctgtcgatga actcggtggc gtgcgcggtg
    15721 aacatcaaga ggccgctcgg gatgatcagc aggaagctgc agagggtcca aggcaacacg
    15781 tgcttcgcca aggttctcgc ggacatgtgc cgggaaaagc ccagcagccg cacgtccagc
    15841 accgcgatcg agccgaacag gagcccgatc ccgacgatgt gaatcgtctc cacgctcggg
    15901 tagagccaca gccactgccg catcgcctgc ccgaggccgg aagcctcgag cgcgcccaga
    15961 gcgccgcccg gcgccgggct catcgcaact ctatggtttt gccattgatc gtgatgcgct
    16021 cggcccgcat ctcttcgggc ttgttgcggt tgggatagcc ctcgacggtc gccgcggcgc
    16081 ccgccttgag ccattccttc ggcagcccgc ggttctccat gcgcgacggc ggcgcgagcg
    16141 tcacggtcca ggtcttgcct gccgtggccc cttctccctt ggcctcgaag cggatgtggc
    16201 cgtgcgggtg ctcatagccc gactcgacga tcttgccggt caacttgagc gcgttcgagg
    16261 tgtcgtactc gctccagccg tggtgcgccg aaaccgccag cggaagtgcg aacaatcctg
    16321 cgacaaacag ccttttcata cgcgttgctc cacccaggat tttacggatt gcagcgctac
    16381 cggcagcttc gccggctcgg tgccgccggc ctgcgccatg tccggccggc cgccgccctt
    16441 gccgcccacc tgctgtgcta cgtagttgac aagctcgccc gccttgagct tcccggtcag
    16501 gtcgtccgtg acgccggcga tgagcgccac cttgccgtcg ttcacggcgc cgagcacgat
    16561 ggctgcggac ttgaggcgat ccttcagctt gtccatggtc tcgcggagcg tcttcgcatc
    16621 ggcgccgtcg agcgtggcca ccagcacccg cgttgccgcc ccgtccttgc cctttacgtc
    16681 caccgcctgc gacgcgaggt ccgtgccctg ccccatcgcc agcttcgagc gcgcccgcgc
    16741 cagctccttt tcgagcgcct ttttctcgtc gagcaggctt ttcacggctt tttcgaccat
    16801 cccggcgccc ggctgcgcgc gcaattccct cgcgacttcg tggaattcct cgagctggcg
    16861 attcatcagc gcgagcgcgt tcgcgccggt cgtcgcctcg acgcggcgga tgccggcggc
    16921 cacgccgcct tccgaagtga tcttgaatac gccgatgtcg ccggtgcgac cgacatgcgt
    16981 gccgccgcag aattcgcgcg agctgccgat ctccagaacg cgcacctcgt cgccgtactt
    17041 ttcgccgaac agcatcatcg cgcccgactt cttggcttcc tcgatcggca tgacctgggc
    17101 gcgcgtcggc gcgttttgca tgatctcggt attcactcgc agctcgactt cgcggatctg
    17161 ctggtcggtc accggcttgt cgtgggagaa gtcgaagcgc gtcttgtcgg cgtccacgag
    17221 cgagcccttc tgctgcacgt gcgaccccag cacctcgcgc agcgccttgt gcatcaggtg
    17281 cgtaacggag tgattgcgca tggtgctcgc gcgcagcgtc gtattcacgc gcgcctcgac
    17341 cttgtcgccc accttcagcg tgccggtctt gagcgtgccc tggtgtccgt acacgtcggc
    17401 ctggatcttc tgggtatcgg cgacctcgaa ggtgccgccg gtgccgacca gctcgccccg
    17461 gtcgccgacc tggccgccgg cctcggcata gaacggcgtg cggtcgagca ccaccgtgcc
    17521 ggaatcgccg cttttcagcg cctgcgcctg cgcgccttcc cggtagagcg cgacgacctt
    17581 ggccggaagc gcaagcgtgt cgtagccgtg gaactcggtc ttccctccct ggtactcgag
    17641 ggtggatgcc gcggaaaact tcgacgctgc ccgcgcccgt tcgcgctgct ggtccatcgc
    17701 ggcctcgaag cccgccatgt cgatgcgcac cccgcgctcg cgcgcgatgt ccgccgtgag
    17761 gtccaccggg aagccgaacg tgtcgtagag gcggaacacg gtctcgccgt cgagcagctt
    17821 ctcgcccgag gcgagcgccg actcgagcac cgtcatgccg ttttccagcg tctcggcgaa
    17881 gcgctcctcc tcctgcttca gcacctcagc gacacggccc ttggcctttc tcaattcggg
    17941 ataggcatcg cccatcgccc ggtcgagatc gtcgaccatg cggaagaaga acggctgctt
    18001 ctgcccgagc ttgtagccat ggcgaatggc tcgtctcacg attcttcgaa gaacgtatcc
    18061 ccgcccctcg ttccccggaa tcacgccgtc gacgatcagg aagctgcagg cgcggatatg
    18121 gtccgcgatc acgttgagcg aagggttctt caagtcgccc tgcttcgttg agccgattgt
    18181 ctccctatgg gccgccttga tcagatcctg gaacagatcg atctcgtagt tagagtgctt
    18241 gccctgcagc accgcggcga tgcgctcgag gcccatgccg gtgtcgaccg acggcttggg
    18301 aaggggatgc atcacgccct tctcgtcgcg gttgaactgc atgaacacga ggttccagac
    18361 ctcgatgtag cggtcgccgt ctgcctcggg cgagcccggg ggaccgccct cgatgccggg
    18421 cccgtggtcg tagaagatct cgctgcacgg cccgcaaggg ccggtgtcgg ccatctgcca
    18481 gaagttgtcc gactggtact tctggccccc ggccttgtcg ccgatgcgca cgacgcggcc
    18541 ggccggcagg ccgatcatct tcgtccagag ctcgtaggcc tcgtcgtcgg tctcgtacac
    18601 ggttgcccac agccggtcgg gctgcagctt gtacaccctg gtcagcagct cccacgcgaa
    18661 ctggatcgcg tccttcttga agtaatcgcc gaaggagaag ttgccgagca tctcgaagaa
    18721 cgtgtggtgg cgtgccgtgt agccgacgtt ctcgaggtcg ttgtgcttgc cgcccgcccg
    18781 aagcgagcgc tgcgcggtcg tcgcgcgctt gtaggggcgc ttgtcctggc cgaggaacac
    18841 gtccttgaac tgcaccatgc cggaattgac gaacagcagc gtcgggtcgt tcgccggcac
    18901 gagcgagctc gaagggacca cggtgtgccc cttggagcgg aaatactcga ggaatccgtt
    18961 gcgaatcgcg ttcgacttca ccgaaccatt ctactgcacc gcaaaaagcc ggggcgccgc
    19021 ggacgccccg gaaccgcagt tctaagcgtt gatgcgcccg tacttgagca acctcgcccg
    19081 cacggctccc atcgtgcgct cgtgcgccgc cgccagctcc tgcagcgcgc gcccggcgtc
    19141 gaacgccgcg agcagcttgc ggtcctcgtc ctcgctccac ggctcgccgg ttttcggcgg
    19201 catctggctg cggcgccgct cgaagcgctc gatgcgctcg agcgccgcgg ctgcctcgta
    19261 cagcgcgcgc accacctgcg gacgctggta actgctgtcc gcagcgaaca cctcgccgct
    19321 ctccggatcg acgccgttcg ccagcgatcg aacgatcgcc aatgcttgtg cctgatccat
    19381 gccctcacct cctctctcag gccgaagcct gtccgtagat gaacgtatgt acagtagtca
    19441 tggatgacag gcggcgctat cttcggactc aaagcaaaaa cggcatgcgg ttcgcaaaaa
    19501 acgacaggcg gtcttgccat ctgattggcc tggcattggc ctggatgctc gcgccggcgg
    19561 cagcgctggc ccaggccgtc accgacgccg agcttcagaa gcgggcgaac gccatcctga
    19621 cgctgatggg cttcgcggtc acgccggatg tcaccaccgg ctccctttcc ctcacggacc
    19681 agacggcagg gaatcccttt tttcgccaga cctccctcag cggcggcgga acgctgccag
    19741 gacgccggct ctatctcgag ggaacgctcg cgtacggtcg ctacgacccg gacttcgcca
    19801 cgaacgacga ttcgccagcc tcccgcgtcg ccgtgaagtg ggacagcctg ctcgggaccg
    19861 gcggggtcgg ctgggacttt ccgctcgcgg cgcacctcgt cctgaggccg atcttcaact
    19921 tttccctcgg acgcatcgag agcgagggca tggtcaccgc cgcgccgccc gccacgcgca
    19981 gccccgccct ggagttcctg agcaacggcc ggctcaatgc ctacggcctc ggcggctcgc
    20041 tcatgctcga ctacgagcgc taccgacccg gcaacgagat cgacgtcgag ttgcgctaca
    20101 gcagcatcca tctgcagagc ttcgacagct cgcgggcggt ggaaggcgag gctgatgcgc
    20161 aaacgctctc gctctggtcg cgctatcgcg cacccacggg catgacggcg ctcgagcgcc
    20221 cggtgcgcta cgtgctcgag tacgcgtata cccgcttcct cggcgatctc gacggaacgc
    20281 tcggcttcac ctatgtgaat tcgttcggcg gcggcctcga gctggattcg agcgcccgcg
    20341 acgtctacgt gacgcgctcg cggctcatgg tccgctacaa ggtcggcaac aacgtgaccg
    20401 gctgggcggt cgggcttggg ctgagcttct agacgacgcc gtaccgcgcg gtagcctagt
    20461 cttcgctctg cagcacgagc cggcggatga cgtcgccgga aaaaccacga ccctgaagga
    20521 agcgcgcacg cttcgcgcgc tcctcccgcg tggcggccgg cgcgcggtac ttgcgctcga
    20581 gaatcgcctt ggccttctcc gtttcgttca atccgaccgc agcgcgctcg gcaagctcct
    20641 gcggaattcc tttgctcgtc aggtcctggc ggatcttggc cgcgccgtac ttgcgagcga
    20701 gccagtgcgt tcggacttcg gcaaagcgct cgtcggagag ctgcttcttg ctctccaact
    20761 cgcgcaacag gccttcaagg acttcgggtg attccgcgta gggcgagagc ttgcgggcca
    20821 gctcgtcccg cgaatgctcc ctgcgggcca ggtgtcgcag cgcccgcgct ctgagctcgc
    20881 tcagcgagtc cggttcaagc tgcgcggcgc ttcctctcgg ggaagaagat ttcccagctg
    20941 cgggcatccc cgacaagaag cccgatccca tcgctcggcg gcttgtcgca aggctttgca
    21001 tcgaacaggg cagccagctt ggctgcccgc ggcgaaaaat acgccgtcac cttgtttgcc
    21061 ttgcggtcgt gtcgcgagaa aacggccatg ctcgacggct gacccgatgc ggcgaacagc
    21121 ggcgagaaca tttgctggat cctgagcgac ggcgcgtaag cggcgcggcc gtcgccgagt
    21181 gtcttgcagt accaggtttc catcgcctat ccgcgcttgc gggtcggcag cttctcgact
    21241 ttgctgtctg cctcttctgc cgccgcggcg agcggcgcgg ccttctgcac gccggccttc
    21301 tcgcggatct tgacctcgat ctcgcgcgcg agcgccgggt tctccttgag gaagtcgcgc
    21361 gcgttgtcct tgccctggcc gaggcggtcg ccgttgtaga tgtaccaggc gcccgatttc
    21421 tcgagcaccg cgagattgac gccggtctcg agcacctcgc cttcgcgcga gatgccttcg
    21481 ccgtagagga tgtcgaattc cgcctgcttg aaaggcggcg cgaccttgtt cttcacgacc
    21541 ttgacgcggg tttcggagcc gatcacctcg tcgcccttct tgatcgaacc gatgcggcgg
    21601 atgtccatgc gcaccgaggc gtagaacttg agcgcgttgc cgccggtggt ggtctccggg
    21661 ttgccgaata gaaccccgat cttcatgcgg atctggttga tgaagatcac cagcgtattg
    21721 gtgcgcttga tgttcgcggt gagcttgcgc agcgcctggc tcatcaggcg cgcctgcagg
    21781 cccatctgcg gctcgcccat ctcgccctcg atttcggcct tgggcgtgag cgccgccacc
    21841 gagtcgatga cgatcacgtc gaccgcgccc gagcgcacca gcatgtcggc gatctccagc
    21901 gcctgctcgc cggtgtcggg ctgcgagatc agcaggtccg cggtcttgac gccgagcttg
    21961 gcggcgtagg aaatgtcgag cgcgttctcc gcgtcgatga aggcggcggt gccgccgatt
    22021 ttctgcatct ccgcgatgac ctggagtgtg agcgtggtct tgcccgagga ctcgggcccg
    22081 tagatctcga ccacccggcc gcgcggcagg ccgcctacgc cgagcgccag atccaggccg
    22141 agcgagccgg tggacaccac gtcgatgtcc ttgatcgccg cgtcgtccat ccgcatgatc
    22201 gagcctttgc cgtactgctt ctcgatctgc gacagcgctg cggccaatgc cttagacttg
    22261 ttgtcgtcca tattcctgta cctcttctta ggttgtcggc atctacggca gacgtttagc
    22321 gctgggccaa caccggatac tgtatatacg ctcagtattg tgggacggtt gaccggggct
    22381 gtcaacgacg agaaaaatcc tttaggagac aaaacgatgg cgaaagtagc gttcctcggc
    22441 ctcggcgtca tgggctatcc catggccggc cacctgctca agaaaggcgg ccacgacgtg
    22501 accgtctaca accgcagccc ggcgaaggcg cagcaatggg ccaaggagta cggcggcaag
    22561 cacgccgcca cgccgcgcga ggccgccaag gactgcgatt tcgtcatgat gtgcgtcggc
    22621 aacgacgacg atgtgcgctc cgtcgcctac ggcgacaacg gcgtcctcgc cgggatgaag
    22681 aagggcgcga tcctggtcga ccatacgacc gcctcggcca ccgtggccag ggagctctcc
    22741 cagaaaacca aggagaaagg gatcggcttt gtcgacgcgc cggtctcggg cggccaggcc
    22801 ggcgcggtga acggtcagct cggcatcatg tgcggcggcg agcaggcgac cttcgacaag
    22861 gcgaagccgg tgctcgatac ctacgccaag atgtgcgccc tgatcggcgg gccgggcgcc
    22921 gggcagctca ccaagatggt gaatcagatc accatcgtcg gcatggcgca ggcgctcgcc
    22981 gaagggctcg ccttcgcgaa gcggaacaac ctcgacctgg agaaagtgat ttcggtgatc
    23041 tcgaagggcg cggcgcagtc ctggcagatg gagaaccgct ggaaacccat gagtgagctc
    23101 aaaaccgagg gcttcggctt cgcaatcgag tggatgcgca aggacgtcgg catctgcctg
    23161 gcgcaggcga agcaaagcaa tgcgcgcctg ccggtgacgg cgctggtcga ccagttctac
    23221 gcggcgctcg aagcgcgcgg cggcaagcgc tgggacagca ccgcctcgct gattcagctc
    23281 ctgctgaagg actagggaac ggtgtcgatg cagccgttga gtatgctttt cgtacccagc
    23341 gtgccgagca gcccgcctaa gctaccggtc gtgtagccgc cggtcgtaca accgagcgcg
    23401 aagcgggtgt tggcaaccgt cgaggcattg gcgcgcaatt gccaccgagc cgtggtgccc
    23461 acaccgaggt tgacgccggc accgaacgcc gcgttcaccg cgtttccggt gttctggtcg
    23521 atggtgttcg agccgatttc agcctgcgag ttgcgatcga cctggatgcc attcccgccg
    23581 ttcatgcgga tcgtattgcc ggcgatgtcc gcctgcgac
//
LOCUS       DATBSZ010000103        17785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_493054, whole genome shotgun sequence.
ACCESSION   DATBSZ010000103 DATBSZ010000000
VERSION     DATBSZ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17785
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493054"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1185
                     /locus_tag="VIG70_06680"
     CDS             172..1185
                     /locus_tag="VIG70_06680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8250275.1"
                     /translation="MQFGIFDWVEASARPPAEVYEHKLELASAADRAGFHGFFIAEHH
                     GTPLSIDGSPGALLSAIFQRTRKLRAGALTFCLPWYHPYRFYSEVCMLDQMSHGRLEL
                     GVGRGVSPIESKIFGLHSMDESRERYRETLETFFAACASPTMKINGIEAELHVKPYQR
                     PYPPLWFPSSNLQSIEFTARHGYHTAFIGMHADCKPLFERYRELWEKHRNDPGRHNAH
                     VAVPFLARTQHLVIADTDAEAEKLGLSAYGAWAAHIHHLTRKLGRPDVHKTTPYGEDS
                     AQRLIAGSPSTVLGKLQEMLRLTGANYLLCILSFGDLAAEDAMRSLELFSSEVKPGLS
                     RSS"
     gene            complement(1145..2050)
                     /locus_tag="VIG70_06685"
     CDS             complement(1145..2050)
                     /locus_tag="VIG70_06685"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8250276.1"
                     /translation="MRAKVFPGAQSLPLMAGVQQGIFERHGIKLELLFTQTSQEQRDG
                     LASGDFQVAHAAVDNAVAMVERAKHDVVIVTGGDSSMNELFAQADVRSIADLKGRVVI
                     VDAPNTAYALQAKKILRLNGLKDSDYTIKPVGGTLFRYRAMREDKSHAASMLNPPFSI
                     DAPGAGLKSLGRAVDLVGPYQATGAFVLRKWAAANADMLTRYIAAQIESTRWAVAPAN
                     KDAAVKMLVERFKLQPEVASRTWDLLADPKFGLAADAQFSLDGFRNVLALRAEIEGDW
                     GGTPPAPERYIDLSYYEKARASLRN"
     gene            2172..3518
                     /locus_tag="VIG70_06690"
     CDS             2172..3518
                     /locus_tag="VIG70_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8250277.1"
                     /translation="MLRESIPDLSKCVEPDRVRREVYTDPQIFDLEMERIHERVWIYC
                     GHESQIPKTGDYYTLQIGRQPLLMARGSDGKISVLYNRCPHRGNMMCGDRHGNTGEFF
                     RCSYHAWTFKHDGKLRSIPMMESGYAGTRFGKDNPDCSMKKAARVENYRGFVFASLSA
                     QGPGLLEFLGESRVAFDDMCDRAPEGEVEVVPNCFRVIQHSNWKIFLENQLDALHPSV
                     VHQSTGRAAHDVEKQLAKKTKKNRGEKGQVPLSYHMLSAFATVTIDKWDNFQTLNYPG
                     GHCVLTGYMGLRPQDPDTLAYDKIMIKAYGQKRFEEIIGVNIHHVLIYPGLSVQSPLQ
                     QLRAVRPLGVDRTLTEIWHFRLKGAPEAIYRRSLAYYNLVNSPATLINADDLENFWKC
                     HQGLSSDGGDWVSFGRHFGRDVEKDGHVETGPNMGTSEAPMRGQMKAWAQYMGNGK"
     gene            3515..3991
                     /locus_tag="VIG70_06695"
     CDS             3515..3991
                     /locus_tag="VIG70_06695"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY8250278.1"
                     /translation="MKSQHAVEQFLYKQADLLDGKRWQDWIDLFAPDGIYWMPPDASY
                     KTWDGQPAIFAEDRNLMTVRMNRVLHPDAWSQRPLWGTNHVVSNVVIEKESKTGDVVA
                     RSRFHMMELRRDDVRHFAGSYRHELKKTKGGYRIKLQRVDMTNAQAAYDYVLQVWV"
     gene            complement(3995..4207)
                     /locus_tag="VIG70_06700"
     CDS             complement(3995..4207)
                     /locus_tag="VIG70_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250279.1"
                     /translation="MKANGHDVTNSFWRNDDGSWICIDPVTLEHPKGRIQLAPGTTLL
                     PGEIFMGVDVAAWLDEQDRLRRAAAI"
     gene            complement(4294..4995)
                     /locus_tag="VIG70_06705"
     CDS             complement(4294..4995)
                     /locus_tag="VIG70_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8250280.1"
                     /translation="MPAAAQRRPANRLLAALPPGTYRRMSRTLEHVHLQFGQVLNKAG
                     DALQHVYFPLDSMVSLLAEVDDGDTLEVGLVGREGLTGMPLALGNPTSPVRELVQGTG
                     SALRMPAKRFIAELGRTPKLRRLVDRCIFVAMSTAMQIAACNKSHHLEARLARWLLMV
                     RDRLGSNEFYLTQDFLARMLGVRRAGVTEAAGVLQGRKLIDYSRGKIKLLDVEGLRPA
                     ACSCYQVIRKLENGN"
     gene            complement(5129..5857)
                     /locus_tag="VIG70_06710"
     CDS             complement(5129..5857)
                     /locus_tag="VIG70_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8250281.1"
                     /translation="MAHTNVPEPRSNSILAALPERYYDRLSKHLEPVELPLGSTLYEA
                     GSRVRRLYFPTHGIVSLLQVMADGASAEIAVVGNDGVVGIAMFMGGETTPSRAVVQSV
                     ARAYSMTADVLKDEFARGGMLQFLLLRYTQALITQMSQTAVCNRHHSVEQQLCRWLLM
                     SIDRIGKNEDLVMTHELIANMLGVRRGGVTVAASKLQDEGLIRYSRGRIEVIDRPKLE
                     KRVCECYAVVKKEYDRLALLRPRR"
     gene            5978..6160
                     /locus_tag="VIG70_06715"
     CDS             5978..6160
                     /locus_tag="VIG70_06715"
                     /inference="COORDINATES: protein motif:HMM:NF027273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HEY8250282.1"
                     /translation="MNRAIETLGSAERLARALGVSVAEIHSWAAGLSDPTPGAFLKAI
                     DIVARPGYFPPASEQS"
     gene            6468..7112
                     /locus_tag="VIG70_06720"
     CDS             6468..7112
                     /locus_tag="VIG70_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250283.1"
                     /translation="MDHSSSVLRSRNNRWTIERIARLAQREVTQLRTNAEQLGQAAVV
                     ALCDQVLLLERPVVLAEKAGRRLKAKHPRLVTRRNAFETRGVMLSAPSSWGGIRPSDG
                     TVVMSIWKDDIRSENGLCRYLLWAPNEKGSRPWSDKPDGRERLEHCRQACERGSAEAL
                     LVYGEPLAGHIPEDRAATVSGIDPAVVLHVEVVLRGREYWAIWGGRRTGVAASL"
     gene            7293..7541
                     /locus_tag="VIG70_06725"
     CDS             7293..7541
                     /locus_tag="VIG70_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250284.1"
                     /translation="MRLFVNPFAIWTGLAFKTGEAMLASVHAASARANAPRVAVIAAA
                     DAPPAKQVRQASKRPRAKAAPAKVRSKTRTGGRRKTAR"
     gene            complement(7545..15725)
                     /locus_tag="VIG70_06730"
     CDS             complement(7545..15725)
                     /locus_tag="VIG70_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY8250285.1"
                     /translation="MVATPLRRSQAAAESPLRSELYSADQMERHGEVLAAAHKLRPGR
                     TRDVLLKRLAENEGVLVATGELLKAAVTAGRAIAPAGEWLLDNFHLIEEQVRTAKRHL
                     PEAYSRELPRLLEGPSAGLPRVYDIALEAIAHGDGRLDAEGLRRFVAAYQTVGPLKLG
                     ELWAIPIMLRLALIENLRRVAARIAAGTSERDLAAAWADRMIEIAGRDAKSLILATAD
                     MARSNPPMASSFVAELARRLQGQGAALALPLTWIEQRLFELNLTIEQLVQSQTQEQAA
                     DQVSMANSIGSLRLLGAMDWREFVEAMSVVEQTLRADPVYGAMDFATRDRYRHAIEAM
                     AKASPLAEGEVAREAIRLAHAAAAHVGFYLIDQGLPELERTARVRVSVLDALRRSSRR
                     NSLALYLGAIALVTGIFSAGLVLGYAGGAREWLLVLIGLVSALATSQLAVTLVNRLAT
                     ALAAPEPLARMDFRGGIPPPSRTLVVVPAMLAGAEHIERLVEALEVRFLANRDENLHY
                     ALLTDFQDADAETLPGDEALALAAFHGIDELNRKYRSAERFFLFHRPRRWNPGERRWM
                     GYERKRGKLSQLNSCLRDGRTDRFALVAGETKVLRGVKYVITLDTDTQLPRDAARQLV
                     GAMAHPLNRYGILQPRMAVSLPATSQSRYAQLFGAEPGIDPYTRAVSDVYQDLFGEGS
                     FIGKGIYDVDAFERALEGRFPENRILSHDLLEGCYARAGLLSDVELYEEYPSRYSADV
                     SRRHRWIRGDWQIAGWLLRRVPTEGAGRFKNPLSWLSQWKIFDNLRRSLAPLALVALL
                     FAGWGLAVLAIVLTPALLASFLEMLRKPIDVPLRRHLAAALPSSGRHLAQAAFTLACL
                     PYEAFFSLDAIVRTIGRMLFTHTRLLEWTASSDLAGERNDGVSSFRAMWIGPVTAIAA
                     TLWLVAWSPVALAAAAPILLLWLASPAIAWWLSRPLGRRGARLTPDQITFLRTAARKT
                     WAFFETFVGPEDHWLPPDNYQEHPVAVVAHRTSPTNMGLALLANLSAYDFGYISAGAL
                     IERTDNALSTMRSLERHRGHFYNWYDTRSLKPLPPLYVSSVDSGNLACHLLTLRPGLL
                     ALADAPIVTPRCFEECRQAALDELTHFRLPDDGAIPTLRELASLNNESGRRARERIGT
                     LERLARESGELARMEYGFLVDKARQLLAIGYNVAEHRLDASCYDLLASEARATTFVAI
                     AQGQLPQESWFALGRLLTSARGEPALLSWSGSMFEYLMPLLVMPAYENTLLDRTCHAA
                     VNRQIAYGRECGVPWGMSESGYNTVDAALNYQYRAFGVPGLGLKRGLTEDVVVAPYAS
                     ALALMVAPEAACLNLQRLAAAGLEGRFGFYEAIDYTASRLQRGQSSAVIRSFMAHHQG
                     MVLLSLAHLLLDRPMQKRFQSDARLQATMLLLQERIPRPTAFYLENAGRFDARAASSG
                     PQTPVRVLATPDTPIPEVQLLSNGRYHVMVTNAGGGYSRWKDLAVTRWREDATCDNWG
                     SFCYLRDLASGEFWSSAHQPSLKHAQGYEAIFSEARAEFRRRDHDYDTHTEIVVSSED
                     DIELRRLRITNRAATRRHLEVTTYAEVVLAPGAADALHPAFSNLFVQTEIIRERQAIL
                     CTRRPRSAAERSPWMVHLMAVHGGEAGEISYETDRARFIGRGCTLAAPDAMNRPLSGS
                     EGSVLDPCVAIRSRIALEPQQSATIDMVFGIGETREACLALAGKYQDRNLADRVFDLA
                     STHSWVTLRQINATESDAQLYARLASSVLYANAALRADAGVLMKNRRGQSGLWGYAIS
                     GDLPIVLARIGDAANIDLVRQLVQAHAYWRLKGLAVDLVIWNEDHAGYRQLLQDQITG
                     LIAAGIEAQVMERPGGIFLRRAEQISEEDRVLFESVARAVISDKRGTLAEQISQKRVK
                     ERRLEGRDARWNAPRLRVVHSAPQAPAARPDLAFFNGTGGFSADGREYVITTCPGQAT
                     LASPGHYTPAPWVNVLANPHFGTVVSESGPGYTWSENAHEFRLTPWGNDAVSGASGEA
                     FYLRDQESGEFWSPTPLPCGGPTPYVTRHGFGTSVFEHTEGGIRSELRVFVDLEAAVK
                     FSVLKLANRSGRTRRLSATGYVEWVLGDLRPKTAMHVVTEIDPSSGALYATNAYNTEF
                     PDRAAFFHVDDAARTVSGSRAEFLGRNGTLGNPAAMRRAQLSNKVGAGLDPCGALQVA
                     FELADGQEREIVFTLGIAGARPAAPRAALEKVQQYWERILGAVQVETPDRAFDLLANG
                     WLLYQTLACRLWGRSGHYQSGGAFGFRDQLQDVMALLHAEPRLAREHLLRCAAHQFRD
                     GDVQHWWHPPSGRGVRSHCSDDYLWLPWAASRYVLSTGDGAVLDEPVHFLDGRAVNPE
                     DDSYYDLPARSSESASLYQHCVRAIERGVAVGEHGLPLIGSGDWNDGMDQVGVKGKGE
                     SVWLAFFRHDVLDRFAGIARAHGDAAFAERCLREAVELRRSIEQHGWDGGWYRRAYFD
                     DGSPLGSAGNAECRIDSIAQSWSVLSGAGSAERSRAAMEALDRHLVRREEGLVKLLDP
                     PFDKSASNPGYIKGYVPGVRENGGQYTHAAIWAAMAFAELGDSRRAWELFSMINPVNH
                     AKSAAAVATYKVEPYVMAADVYAAAAHTGRGGWTWYTGSAGWMYRLAVESLLGLRLEG
                     DKLRLAPCLPASWPGFKLRYRYRDTVYRIAVVRDESGSVEHSDHVIPLVDDRGEHRVE
                     VRIRTLILPKQNEN"
     gene            16073..16450
                     /locus_tag="VIG70_06735"
     CDS             16073..16450
                     /locus_tag="VIG70_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEY8250286.1"
                     /translation="MQNPASISRHSVLPLLVVLPVGLWVFSLFCDLLYLGGADAEIWS
                     RLALYTMVGGFIGAFTAVALPGFVVHMNVTLVVVALYAVNIWLRLGDPHLAMAIALSV
                     IGVGMLAVSSWLGSATVRVHGAK"
     gene            complement(16582..16980)
                     /locus_tag="VIG70_06740"
     CDS             complement(16582..16980)
                     /locus_tag="VIG70_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250287.1"
                     /translation="MDYGDEGRITECPGYVRVDFGSCNAAELSAVYRAFTAFCLDNKV
                     TRALLKAGDDHPNGHYALRDALATMARLARIPVDFKLALIPSTRPIEAVYREAQRHLR
                     AAGFNAWVFATENEAVDWLEGRALGGQAAS"
     gene            complement(16986..>17785)
                     /locus_tag="VIG70_06745"
     CDS             complement(16986..>17785)
                     /locus_tag="VIG70_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8250288.1"
                     /translation="VKSGPEREALESGQVDAVLDPETRTAFLLPDAARALSAAGGPIA
                     NSLLAALPREDYQGLLGELEPLTLSSGEVLYEPGERIRHVYFPGDAQVSLLVVMPDRK
                     ALEVGLVGREGMVGIPLVLGAEVSSVRVLVQGSGSALRMKAASFREALARCLPLQREL
                     YRYAYAKLGQARQTAACNRFHQVEARLAGWLLLTRDRVRSDQFHLTHEFVAATLGIRR
                     VGVTNAAGALQRRKLISYRRGNIRILDRKGLKAASCGCYEIVGKLAS"
BASE COUNT         3015 a         5942 c         5966 g         2862 t
ORIGIN      
        1 cctgtgcact tcgctgatgt cgccgctgac gaccgccgag tggagcaacg tgtttccgaa
       61 cgggccttga gtgttgggat tgccgagggt gcgattcatg cgcccgatct ttgcgtatat
      121 agatgacaac tagatacagc aagcggttcc cctcgtatta cgattgaaac catgcaattt
      181 ggaatcttcg actgggtcga agcctcggca cgcccgcccg ccgaggtcta cgagcacaag
      241 ctcgagctcg catcggccgc ggaccgcgcg gggttccacg gctttttcat cgccgagcac
      301 cacggcacgc cgctctcgat cgacggctcg ccgggcgcgc tgctctcggc gatcttccag
      361 cgcacgcgca agctccgcgc cggcgcgctg accttctgcc tgccctggta ccatccttac
      421 cgcttctaca gcgaagtgtg catgctcgac cagatgtcgc acggcaggct tgagctcggg
      481 gtcgggcgcg gggtgtcgcc gatcgagtcg aagattttcg gtctccattc aatggacgaa
      541 tcgagggagc gataccgcga aacgctggaa acgttcttcg ccgcctgcgc ctctcccacc
      601 atgaagatca acggcatcga ggctgagctg cacgtgaagc cctaccagcg gccctatccg
      661 ccgctgtggt tcccgagctc gaacctgcag tcgatcgagt tcaccgcccg ccacggctat
      721 cacaccgcgt tcatcggtat gcacgccgac tgcaagccgc tcttcgagcg ctaccgggag
      781 ctatgggaga agcaccggaa tgaccccgga cggcacaacg cgcatgtcgc agtgccgttc
      841 ctggcccgca cgcagcacct cgtcatcgcg gacaccgatg ccgaggcgga aaagctgggg
      901 ctgagcgcct atggcgcctg ggccgcgcac atccaccacc tcacgcgcaa gctcgggcgg
      961 cccgacgtgc acaagaccac gccttacggc gaggattccg cgcagcggct gatcgccgga
     1021 tcgccgagca cggtgcttgg gaagctgcag gaaatgctgc ggctgaccgg cgcgaactac
     1081 ctgctctgca tcctctcctt cggcgacctc gcggccgagg atgcgatgcg ctcgctcgag
     1141 cttttcagtt ccgaagtgaa gcccgggctt tctcgtagta gctgaggtcg atgtagcgct
     1201 cgggcgcggg cggcgtgccg ccccagtcgc cctcgatctc ggcgcgcagc gcgagcacgt
     1261 tgcggaagcc gtcgaggctg aactgcgcat ccgcggcgag gccgaatttc gggtcggcga
     1321 gcaggtccca ggtgcgcgag gccacctcgg gctggagctt gaagcgctcg accagcatct
     1381 tcaccgcggc gtccttgttc gccggcgcca ccgcccagcg cgtcgactcg atctgcgccg
     1441 cgatgtagcg agtgagcatg tcggcgttgg ccgccgccca cttgcgcagc acgaaggcgc
     1501 cggtcgcctg gtaggggccg acgaggtcca ccgcgcggcc gaggctcttc agcccggcgc
     1561 ccggcgcgtc gatcgagaac ggcgggttga gcatcgaggc ggcgtggctc ttgtcctcgc
     1621 gcatcgcgcg gtagcggaac agcgtcccgc cgaccggctt gatcgtgtag tcggaatcct
     1681 tgaggccgtt caggcgcagg atcttcttcg cctgcagcgc gtaagcggtg ttcggcgcat
     1741 cgacgatcac cactcgcccc ttgaggtcgg cgatcgagcg cacgtcagcc tgggcgaaaa
     1801 gctcgttcat gctcgagtcg ccgccggtga cgatcaccac gtcgtgcttc gcgcgctcga
     1861 ccatcgccac cgcgttgtcc acggcggcgt gcgctacctg gaagtcgccc gaggcgaggc
     1921 cgtcgcgctg ctcctgggaa gtctgggtga agaggagctc gagcttgatc ccgtggcgct
     1981 cgaagatccc ctgctgcacc ccggccatca gcggcagcga ctgcgcgccc ggaaacacct
     2041 tggctcgcaa tggggtttga ccaagcgcat taccaaggat taggagtgac aggcaaaaaa
     2101 cgaatccgcg gatcatggcg cctcctccag ggggacggct agattatcat tggctccgag
     2161 gagaaaacat catgctgcgc gaatccattc ccgatctttc caagtgcgta gagcccgacc
     2221 gggtgcggcg cgaggtgtac accgaccccc aaatatttga cctggagatg gagcgcatcc
     2281 acgagcgcgt gtggatctac tgcggccacg aaagccagat cccgaagacc ggcgactact
     2341 acacgctgca gatcggccgc cagccgctgc tgatggcgcg cggctccgac ggcaagatca
     2401 gcgtgctcta caatcgctgc ccgcaccgcg gcaacatgat gtgcggcgac cgccacggca
     2461 acaccggcga gttcttccgc tgctcctacc acgcctggac gttcaagcac gacggcaagc
     2521 tgcgctcgat cccgatgatg gagtcgggct acgccggcac ccgctttgga aaggacaacc
     2581 ccgactgcag catgaagaag gccgcgcgcg tcgagaacta ccgcgggttt gtttttgcaa
     2641 gtctgtccgc tcaggggccg ggcctgctcg aatttcttgg agagtccagg gtcgcgttcg
     2701 acgacatgtg cgaccgcgcc cccgagggcg aggtggaggt ggtgccgaac tgcttccgcg
     2761 tcatccagca ctcgaactgg aagatcttcc tcgagaacca gctcgacgcg ctgcacccct
     2821 cggtggtgca ccagtccacc ggccgcgccg cgcacgacgt ggagaagcag ctcgcaaaga
     2881 agacgaagaa gaatagaggg gagaaagggc aggtgccgct ctcctaccac atgctctcgg
     2941 ccttcgcgac cgtgaccatc gacaagtggg acaacttcca gaccctcaac tatccgggtg
     3001 gccattgcgt gctgaccggc tacatgggcc tcaggccgca ggaccccgac acgctcgcct
     3061 acgacaagat catgatcaag gcgtacggcc agaagcgctt cgaggagatc atcggcgtca
     3121 acatccatca cgtcctcatc tacccgggcc tgtcggtgca gtcgcccctg cagcagctcc
     3181 gcgcggtgcg gccgctgggc gtcgaccgca ccctcaccga gatctggcac ttccgcctga
     3241 aaggagcgcc cgaggcgatc taccgccgct cgctcgccta ctacaacctg gtgaactctc
     3301 cggccacgct gatcaacgcc gacgacctgg agaacttctg gaaatgccac caggggctct
     3361 cgtcggatgg cggcgactgg gtcagcttcg gccggcattt cggccgcgac gtcgagaaag
     3421 acggacacgt cgagaccggc ccgaacatgg gcacctccga agcgccgatg cgcgggcaga
     3481 tgaaggcctg ggcgcagtac atggggaatg gaaagtgaag agccagcacg cggtagagca
     3541 gtttctctac aagcaggccg atctcctcga tggcaagcgc tggcaggact ggatcgatct
     3601 ctttgccccc gacggcatct actggatgcc gccggacgcc tcgtacaaga cctgggacgg
     3661 gcaaccggcg atcttcgccg aagacaggaa cctgatgacc gtgcgcatga accgcgtgct
     3721 gcacccggac gcctggtcgc agcggccgct ctggggcacc aaccacgtcg tttcgaacgt
     3781 ggtgatcgag aaggaatcga agaccggcga cgtcgtggcc cgctcgcgct tccacatgat
     3841 ggagctgcgc cgcgacgatg tgcgccactt cgcgggctct taccggcacg aactgaagaa
     3901 gaccaagggc ggttaccgca tcaagctgca gcgcgtggac atgaccaacg cgcaggcggc
     3961 gtacgactac gtgctgcagg tctgggttta gagcctatat cgccgcagcg cggcgcagcc
     4021 gatcctgctc gtcgagccag gcagcgacat cgactcccat gaagatttca ccgggcagca
     4081 gcgtcgtgcc gggggcgagc tggatcctgc ctttgggatg ctcgagggtg acggggtcaa
     4141 tgcagatcca cgaaccgtcg tcgttccgcc agaaggaatt cgtgacgtcg tggccgttcg
     4201 ccttcattcg cccgagcctc agtgcctgtc ccaaaaaaca tatgcccttc tttttcgagc
     4261 tgcaacattt gttcggtagc gtacctaagc ccctcagttg ccgttttcca gcttgcggat
     4321 cacctggtag caggaacacg cggccgggcg caggccctcc acatcgagca gcttgatctt
     4381 gccgcggctg tagtcgatca gtttgcggcc ctgcagcacg cccgccgcct cggtgacccc
     4441 ggccctgcgc acgccgagca tccgggcgag gaaatcctgg gtgaggtaga actcgttgct
     4501 ccccagccgg tcgcgcacca tgagcagcca gcgcgcgagg cgcgcttcga ggtggtgcga
     4561 cttgttgcag gccgcgatct gcatggcggt gctcatcgcc acgaaaatgc agcgatcgac
     4621 gagccgccgc agtttcggcg tacgaccgag ctcggcgatg aagcgcttgg cgggcatgcg
     4681 cagcgcgcta cccgtgcctt ggaccagctc gcgcaccggc gaggtcggat tgccgagcgc
     4741 gagcggcatc ccggtcaggc cctcccggcc gacgagcccg acttcgaggg tatcgccgtc
     4801 gtcgacctca gcgagcagcg agaccatgga atcgagcggg aaatacacgt gctgcagcgc
     4861 atcgcctgct ttgttcagca cctggccgaa ttgaaggtgg acatgctcca gggtgcgcga
     4921 catgcgccgg taggtgcccg gcggcagcgc cgcgagcagg cggtttgcgg gtcggcgttg
     4981 cgccgcggcc ggcatggaga ctcccggttg acgtcagttc cagtggactg acggcaggag
     5041 ccctgaaagg ctgacccggt gtcgagagcc gactgcttgg atgaagctag tacggccagt
     5101 gtacgctcag tgacggacga ccactgcgct atcttcgcgg cctgaggagg gcgagccggt
     5161 cgtattcctt cttgaccacc gcgtaacact cgcaaacccg cttctcgagc ttcggccggt
     5221 cgatgacctc gatgcggccg cgggaatagc ggatcagtcc ttcgtcctgc agcttgctcg
     5281 ctgccaccgt cacgcctccg cggcgcaccc caagcatgtt ggcgatcagc tcatgcgtca
     5341 tgaccaggtc ttcgttcttg ccaatgcggt cgatgctcat cagcagccag cggcagagct
     5401 gctgctctac gctgtgatgg cggttgcaca ccgcagtctg cgacatctgg gtaatcagcg
     5461 cctgggtgta gcgcagcagc aggaactgca gcatgccgcc gcgcgcgaac tcgtccttga
     5521 gcacgtcggc agtcatcgaa tacgcgcgag cgacgctctg cacgacggcg cgcgacggcg
     5581 tggtttcgcc tcccatgaac atcgcgattc caactacacc gtcgttgccg accaccgcga
     5641 tctctgccga agcgccgtcc gccatcacct gaaggagcga gactatgccg tgggtaggga
     5701 aatagagccg ccgcacccgg cttccagcct catagagcgt ggatccgagc ggcaactcga
     5761 cgggctcgag atgcttgctt agccggtcgt aatagcgctc ggggagtgcc gcaagaatgg
     5821 agttcgaacg cggctcaggg acgttggtat gggccataac gtacatgcga ttttaacccg
     5881 acggacctgc catttgtacg ataccggaca gactcgtgcg agcccgaggc ggatactttg
     5941 ctagcttatg tccggcgaca ccgcctatac gcgaaccatg aaccgtgcca tcgagacgct
     6001 cgggagcgcc gagcgtctgg cgcgcgcgct cggcgtttcg gtcgccgaaa tacattcgtg
     6061 ggcagcaggg ctttcggatc caacgccggg cgcgttcctc aaggcgatcg acatcgtcgc
     6121 gcggccgggc tactttccgc ccgcgtcgga acaatcctga cttcctagtg cgctgagctt
     6181 tcctcgagct ccacgccgag cgcaatcagg cgcgtgacgc gctgcgcaag gtcctcttcc
     6241 tgcgctggat ggcgtcgagc tcaccgcgct catcaaagcg ctgcccaatt ctccctcgat
     6301 cgtcgtgatc accgccggct ccgctgcggg cgtcgatctg ctgctggaga agcatcacca
     6361 cgagaagcct ggcgagcttg cacgagcgcc gcgtttagct cagcgggtcg atctccaagg
     6421 aatagggggc gaccaggtgc gaatcagcgt ataagaaaga gactgccatg gaccattctt
     6481 cttccgtgct tcgttcacgc aacaatcgat ggacgatcga gcgaatcgcc cggctcgcgc
     6541 aacgcgaagt aacccagctg cgcacgaacg cagagcagct cgggcaggcg gcagtcgtgg
     6601 cgctttgcga ccaggtactg ctgctcgagc gcccggttgt tctcgcggag aaggcggggc
     6661 gccggctgaa agcgaagcac ccgcgcctcg tgacgcggcg aaacgcgttc gagacgagag
     6721 gcgtgatgct gtccgcgccg tcgagctggg gcgggatccg gccctcggac ggcaccgtgg
     6781 tgatgtcgat ctggaaggac gacatccgca gcgaaaacgg cctgtgccgc tacctgctgt
     6841 gggcgccgaa cgagaaaggc tcgcgcccgt ggtcggacaa acccgacggc cgggagcgcc
     6901 tggagcattg caggcaggcg tgcgagcgcg gctcggcgga ggcgcttctc gtgtacggag
     6961 agcccctcgc cgggcacatt cctgaagacc gggccgcgac ggtgtcgggc atcgatcctg
     7021 cggtcgtgct gcacgtcgaa gtggttcttc gcggccgcga atactgggcg atctggggcg
     7081 gccgcagaac cggcgtcgcc gcgtcgcttt gagcccgggc gacctcgccg cggtgcgcaa
     7141 gccttagttt ctcggaaatc caagaataag ctcgtgtatg tgcgccagca ggcagcgcaa
     7201 ctgttggcga gcgccaacaa tggctgcgga ggtagtccaa tgaaaacgat gtcgactcgc
     7261 gaagcactat gaactcgaaa ccggagaagt ctttgcgcct gttcgtaaac ccgttcgcga
     7321 tctggaccgg tctcgcgttc aagaccggcg aggcgatgct ggcgtcggtg catgccgcgt
     7381 ccgcgcgcgc gaatgccccg agagtggccg tgatcgctgc cgccgacgcg ccgccggcga
     7441 agcaggttcg gcaagcatcg aagcggcctc gtgccaaagc cgcgccggca aaggtgcgca
     7501 gcaagacaag gacaggcggt aggcggaaaa cggcgcgcta agcgctagtt ctcattctgt
     7561 ttcggcagga tcagcgtccg gattctcact tcgacgcgat gttccccgcg atcgtcgacc
     7621 agcgggatga cgtggtctga gtgctcgacg ctgccgcttt cgtcgcgcac tacggcgatg
     7681 cgataaaccg tatcgcggta gcggtagcgt agcttgaacc cgggccagct cgccggcagg
     7741 cacggcgcga ggcgcagctt gtcgccttcg agccgcaggc ccaggagcga ttccaccgcg
     7801 agccggtaca tccagcccgc agacccggtg taccaggtcc agccgccgcg gccggtgtgc
     7861 gcggccgccg catagacatc cgccgccatc acgtacggct ccaccttgta ggtggcaact
     7921 gctgccgcgg atttcgcgtg gttgaccggg ttgatcatgg agaagagctc ccatgcgcgg
     7981 cggctgtcgc ccagctcggc gaatgccatc gccgcccaga tcgcggcatg cgtgtactgc
     8041 ccgccgttct cccgcacccc cgggacgtag cccttgatat acccgggatt cgacgccgac
     8101 ttgtcgaacg gcggatcgag cagcttgacc agcccctcct cgcggcgaac caggtggcga
     8161 tcgagcgcct ccatcgcggc gcgcgagcgc tccgcgctgc cggcgccgga gagcaccgac
     8221 cagctctggg cgatcgaatc gatgcggcat tccgcgttgc ccgccgagcc gagcggcgag
     8281 ccgtcgtcga agtacgcgcg gcggtaccac ccgccatccc agccgtgctg ctcgatactg
     8341 cgtcgcagct cgaccgcttc gcgcaggcag cgctcggcga aggcggcgtc gccatgggcg
     8401 cgcgcgatcc cggcgaagcg atcgagcacg tcatggcgga aaaaggcgag ccagacgctt
     8461 tcgcccttcc ctttcacgcc cacctggtcc atgccgtcgt tccagtcgcc cgacccgatg
     8521 agcggcagcc cgtgctcgcc gacggcgact ccgcgctcga tggcgcgcac gcagtgctgg
     8581 tacaggctcg ccgattcgct cgagcgcgcg ggcaggtcgt aataggagtc gtcctcgggg
     8641 ttcaccgcgc ggccgtcgag gaagtggacc ggctcgtcga gcaccgcgcc atccccggtg
     8701 ctcagcacgt agcgcgacgc agcccagggc agccagaggt aatcgtccga gcaatgcgag
     8761 cgcacgccgc gtcccgaggg cggatgccac cagtgctgca cgtcgccgtc gcggaactga
     8821 tgcgccgcgc agcgcagcag gtgctcgcgg gcgaggcgtg gctccgcatg aagcagcgcc
     8881 atgacgtcct ggagctggtc gcggaagccg aaagcgccgc ccgactggta atggccgctg
     8941 cgcccccaga ggcggcacgc cagcgtctgg tacaggagcc agccgttcgc cagcaggtcg
     9001 aaggcccggt cgggcgtttc cacctgcact gcgcccagga tgcgctccca gtattgctgc
     9061 accttttcca gcgcggcgcg cggcgccgcc ggtctcgccc cggcgatgcc gagggtgaag
     9121 acgatctcgc gctcctgccc gtcggcgagc tcgaaagcga cctgcagcgc gccgcaggga
     9181 tcgagccccg cgccgacctt gttcgagagc tgcgcgcggc gcatcgcggc cgggttcccg
     9241 agcgtgccgt tgcgtccgag aaactcggcg cggcttccgc tcaccgtgcg ggccgcatcg
     9301 tcgacgtgga agaacgctgc ccgatcgggg aactcggtgt tgtaggcgtt ggtcgcgtag
     9361 agcgcgccgc tcgagggatc gatctcggtg accacgtgca tcgccgtttt cggccgcagg
     9421 tctcccagca cccactcgac gtagcccgta gccgagagcc ggcgcgtgcg gcccgagcgg
     9481 ttggcgagct tcagcaccga aaatttgacg gccgcctcga ggtccacaaa gacgcgcagc
     9541 tcggagcgga tgccgccctc ggtatgctcg aagacgctgg tgccgaagcc gtgccgcgtg
     9601 acgtagggcg tgggcccgcc gcacggcagc ggcgtcggcg accagaactc tccgctctct
     9661 tggtcgcgca ggtaaaaggc ttctccgctc gccccgctca ccgcatcgtt cccccagggg
     9721 gtgagccgga actcgtgggc gttctcgctc caggtgtagc ccgggccgct ctccgacacc
     9781 acggtgccga agtgcgggtt cgccagcacg ttcacccacg gcgccggcgt gtagtgaccg
     9841 ggcgacgcta gcgtcgcttg accggggcaa gtcgtgatca cgtactcgcg tccgtcggcg
     9901 ctgaacccgc cggtgccgtt gaaaaaggcg agatcggggc gcgccgctgg cgcctgcggc
     9961 gcggagtgca cgacccgcaa gcgcggagcg ttccagcgcg cgtcgcgccc ttcgaggcga
    10021 cgctccttta cgcgcttctg gctgatctgc tcggccagcg ttccccgctt gtcgctgatg
    10081 acggcacggg cgaccgattc gaaaaggacg cggtcctcct ccgagatctg ctccgcccgg
    10141 cgcaggaaga tgccgcccgg gcgctccatc acctgcgctt cgatgcccgc ggcgatcagc
    10201 cccgtgatct ggtcctggag caattgccgg taaccggcgt ggtcctcgtt ccagataacg
    10261 agatccaccg ccagtccctt caggcgccag tacgcatggg cctggacgag ctggcgcacg
    10321 agatcgatgt tcgccgcgtc cccgatgcgc gcgagcacga tcggcaggtc gccggaaatg
    10381 gcgtagcccc agagccccga ttggccgcgg cggtttttca tcagcacgcc cgcatcggcg
    10441 cgcagcgcag cgttggcgta gagcaccgag ctcgcaaggc gtgcatagag ctgggcgtcg
    10501 gactcggtgg cattgatctg gcgcagcgtg acccagctgt gcgtcgacgc caggtcgaac
    10561 acacggtccg cgagatttcg gtcctgatat ttgcccgcca gcgcgaggca agcctcgcgc
    10621 gtctcgccga tgccgaaaac catgtcgatc gtcgccgatt gctgcggctc cagcgcgatg
    10681 cggctgcgga tcgcaacgca cggatcgagg acggagccct cgctccccga gaggggccgg
    10741 ttcatagcgt cgggcgcagc cagcgtgcat ccgcgcccga tgaagcgcgc gcgatcggtc
    10801 tcgtaggaga tttcgccggc ctcgccgccg tgcaccgcca tcaggtgaac catccagggc
    10861 gagcgctccg cagccgagcg cggccgccgg gtgcagagga tcgcctggcg ctcgcggatg
    10921 atctcggtct ggacgaagag gttgctgaag gccggatgca gggcgtcggc cgcgccgggc
    10981 gcgagcacga cttcggcgta ggtcgtgacc tcgaggtgcc ggcgcgtcgc ggcgcggttg
    11041 gtgatgcgca ggcgccgcag ctcgatatcg tcttccgacg agaccacgat ctcggtatgt
    11101 gtatcgtagt cgtggtcgcg gcgccggaac tcggcgcgcg cctccgagaa gatcgcttcg
    11161 tagccctgcg cgtgcttgag cgagggctga tgggcgctcg accagaactc cccgctcgcc
    11221 aggtcgcgca ggtagcagaa gctgccccag ttgtcgcaag tagcgtcctc gcgccagcgg
    11281 gtgacggcaa gatccttcca gcggctgtag ccgccgccgg cattcgtgac catgacgtga
    11341 tagcggccgt tcgacagcag ctgcacctcg gggatcgggg tatccggggt ggcgagcacg
    11401 cgcaccggcg tctgcggccc gctggaggcg gcgcgcgcgt cgaagcggcc ggcgttttcc
    11461 agatagaacg cggtcggcct tggaatgcgc tcctgcagaa ggagcatcgt cgcctgaaga
    11521 cgcgcatcgc tctggaatct cttctgcatc ggacgatcga gcagcagatg ggcgagcgac
    11581 agcaggacca tgccctggtg atgcgccatg aaggagcgga tcacggcact cgactggccg
    11641 cgctgcaggc gtgaagcggt gtagtcgatc gcctcgtaga agccgaatcg gccttcgagg
    11701 cccgcggcgg cgagccgctg caggttcagg cacgccgcct cgggcgccac catcagcgcg
    11761 agcgccgagg cgtagggcgc aaccacgaca tcctcggtca gcccgcgctt cagcccgagg
    11821 cccggcacgc cgaaggcgcg gtactggtag ttgagggcgg cgtcgaccgt gttgtagccc
    11881 gattcggaca tgccccaggg cacgccgcat tcgcggccgt aggcgatctg ccggttcacg
    11941 gccgcgtggc aggtgcgatc cagcagcgtg ttttcgtacg ccggcatcac cagaagcggc
    12001 atcaggtact cgaacatcga gccgctccag gacagcaggg ccggctcgcc gcgggcgctc
    12061 gtcaggaggc ggccgagggc gaaccagctc tcctgcggca gctgcccctg cgcgatcgcc
    12121 acgaaggtgg tcgcgcgcgc ctccgaggcg agcaggtcgt agcagctcgc gtcgagccgg
    12181 tgctcggcca cgttgtagcc gatggcgaga agctggcgcg ccttgtcgac caggaacccg
    12241 tactccatgc gggcaagctc gccggattcc cgcgccaggc gctcgagcgt gccgatgcgc
    12301 tcccgcgcgc gccggcccga ttcgttgttc aggctggcca gctcgcgcag cgtcgggatg
    12361 gcgccgtcgt cgggcaagcg aaaatgggtc agctcgtcga gagcagcctg gcggcactct
    12421 tcgaagcagc gcggcgtcac gatcggtgcg tcggcaagcg cgagcagccc cggccgcaag
    12481 gtcagcagat ggcaggcgag gttcccgctg tccaccgacg agacgtagag cggcggcagc
    12541 ggcttcaggg acctcgtgtc gtaccagttg tagaaatgtc cccggtggcg ctcgagcgac
    12601 cgcatcgtgc tgagcgcatt gtcggtgcgc tcgatgagcg ctccggcgct gatgtagccg
    12661 aagtcgtacg cggacaggtt cgccagcagc gcgagcccca tgttggtcgg cgaggtgcga
    12721 tgcgcgacga cggcaaccgg gtgctcctgg taattgtcgg gcggcagcca gtggtcctcc
    12781 ggtccgacga aagtctcgaa gaacgcccag gtcttgcgcg cggccgtgcg caggaaggtg
    12841 atctgatccg gtgtcaggcg cgccccccgg cgcccgagcg gtcggctcag ccaccacgcg
    12901 atggcgggcg aggcgagcca caggaggaga atcggcgccg cagccgccag cgcgacgggg
    12961 ctccacgcaa ccaaccagag cgttgcggcg atggcggtga ccgggccgat ccacatcgca
    13021 cgaaaggagg agacgccatc gttccgttca cctgcgaggt cgctcgacgc ggtccattcc
    13081 agcagccggg tatgcgtgaa gagcatccgg ccgatcgtgc gaacgatcgc gtccaggctg
    13141 aagaacgcct cgtagggcag gcacgcgagc gtgaaggccg cctgtgccag gtggcgcccc
    13201 gacgaaggca atgccgcggc gaggtgccgg cgcaacggca cgtcgatggg cttgcgcagc
    13261 atctccagga acgaggcaag cagggcagga gtcaggacga tggcgagcac ggccaggccc
    13321 cagcccgcga acagcagcgc caccagcgcc agcggcgcga ggctgcgccg caggttgtcg
    13381 aagatcttcc actgcgacag ccacgagaga gggttcttga aacggcctgc accttccgtg
    13441 ggcacgcggc gcagcagcca gccggcgatc tgccagtcgc cgcggatcca gcggtgccgg
    13501 cggctcacgt ccgcgctgta gcgcgacgga tactcctcgt acagctcgac gtcgctcagc
    13561 agcccggcgc gcgcataaca gccttccagc agatcgtggc tgaggatgcg gttctcggga
    13621 aagcgtccct cgagcgcgcg ctcgaacgca tcgacgtcat agatgccctt gccgatgaac
    13681 gagccttcgc cgaagagatc ctggtacacg tcggaaacgg cgcgcgtgta cgggtcgatg
    13741 ccgggctccg cgccgaagag ctgcgcgtag cgcgactggc tcgtggccgg caggctgacg
    13801 gccatgcgcg gctgaaggat gccgtatcga ttcagcgggt gcgccatcgc gcccaccagc
    13861 tggcgcgccg catcgcgggg gagctgcgta tcggtgtcga gggtgatcac gtacttcacg
    13921 cctcgcagga ctttcgtctc gccggccacg agcgcgaagc gatcggtccg gccgtcgcgc
    13981 aggcaggaat tcaattgcga gagcttgccg cgcttgcgct cgtagcccat ccagcggcgc
    14041 tcgccgggat tccagcggcg cggccggtgg aaaagaaaga agcgctccgc gctgcggtac
    14101 ttgcggttga gctcgtcgat gccgtggaaa gcggcgagcg ccagcgcctc gtctcctggc
    14161 agcgtttccg cgtccgcatc ctggaaatcg gtcagcagcg cgtagtgcag gttctcgtcc
    14221 cggttggcga ggaaacgcac ttcgagcgcc tcgaccagcc gctcgatgtg ctcggcaccg
    14281 gccagcatgg ccgggaccac gaccagggtg cgcgatggcg gcggaatccc gccgcggaaa
    14341 tccatccgcg ccagcggttc gggcgccgcc agcgccgtgg cgaggcggtt caccagcgtc
    14401 accgcgagtt gactggtggc gagagccgaa accaggccga tcaggaccag cagccactcg
    14461 cgtgctccgc cggcatagcc aagcaccaga cccgcgctga agatcccggt gaccagcgcg
    14521 atcgcgccga ggtacagcgc gagcgaattc cggcggctcg acctgcgcag agcatcgagg
    14581 accgaaaccc gcactcgcgc cgtgcgttcg agctccggca gtccctggtc gatcaggtag
    14641 aacccgacgt gcgcggccgc cgcatgcgcc aggcggatcg cttcgcgcgc cacctcgcct
    14701 tcggcgagcg ggctcgcttt cgccatcgcc tcgatcgcgt gccggtagcg gtcgcgcgtg
    14761 gcgaaatcca tcgcgccgta gaccgggtct gcgcgcagcg tctgctcgac gacgctcatc
    14821 gcctcgacga actcgcgcca gtccatcgcc ccgaggaggc gcaggctgcc gatgctgttc
    14881 gccatcgaga cctggtcggc ggcctgctcc tgggtctgcg actgcaccag ttgctcgatc
    14941 gtcagattca gctcgaagag ccgctgctcg atccaggtga ggggcagcgc gagcgcggcg
    15001 ccctgaccct gcaagcggcg cgcgagttcc gcgacgaagg agctcgccat cggcgggttc
    15061 gagcgcgcca tgtccgccgt cgcgaggatc aggctcttcg catcgcggcc ggcgatctcg
    15121 atcatccggt ccgcccacgc ggcggccaga tcccgctcgg aggtgccggc ggcgatgcgc
    15181 gcggccacgc gccggaggtt ctcgatcagc gcgaggcgca gcatgatggg gatggcccac
    15241 agctcgccca gcttgagcgg cccgaccgtc tggtaggcgg cgacgaaacg ccgcaggcct
    15301 tccgcgtcca gccgcccatc gccgtgcgcg atggcctcga gggcgatgtc gtaaacgcgc
    15361 ggcagccccg ccgacggccc ctccagcagg cgcggcagct cgcggctgta cgcctccggc
    15421 agatgccgct tggcggtgcg aacctgctct tcgatcaggt ggaagttgtc gagcagccat
    15481 tccccggccg gcgcgatcgc gcggccggcg gtgacggccg ccttcagcag ctcgccggtg
    15541 gcaaccagca cgccttcgtt ctcggcgaga cgcttcagga ggacatcgcg ggtgcgtccc
    15601 gggcgcaact tgtgcgcagc cgccaggacc tcgccatggc gctccatctg gtctgcgctg
    15661 tagagctccg agcgcagcgg ggactccgcg gcagcctgcg agcggcgcag cggcgtcgcc
    15721 accatcaacg cttcggcgga agcgacttgt ccatgagcag cgtcaggtta gagggaatgg
    15781 cctgcacggt atgtacgctg ccgcacggga agggttcgcc atgacgacga tgtcgtcatt
    15841 ccaagacaac tgctaagcag gctggcgaac agacgcaaga gcgctcgccg cgtagattgg
    15901 gctgacagga gatcagtcat gatcaggacg caagtggaac aacgggcttg gttgaacggc
    15961 cggtcgtggg aagccatgaa ggcgcccgtg ccgcggcccg gggatccggt ccgtgaggcc
    16021 gagcaccgct gggagaacga gggtggcaat ccaccaatga cggagccgcc ggatgcaaaa
    16081 ccccgcaagt atctctaggc actcggttct tccgctgctg gtcgtgctgc cggtcggcct
    16141 ttgggtgttt tcgctcttct gcgacctgct ctacctgggc ggcgccgatg ccgagatctg
    16201 gtccaggctc gcgctctaca ccatggtggg cgggttcatc ggcgcgttta ccgcagtggc
    16261 gttgccggga tttgtcgttc atatgaacgt caccctggtt gtcgtcgcgc tctacgcagt
    16321 gaacatctgg ctgcgcctcg gcgatccgca cctggcgatg gccatcgcgc tctcggtcat
    16381 cggcgtcggc atgctcgccg tgtcgagctg gctcggcagc gcaacggtcc gcgtccacgg
    16441 agcgaaatga gcaagagctc cggcgcgcgc atcaggggtt tcctcggcat ttcgcgatcc
    16501 gcaatgtccg cagggtgctg catcaacgca agctccacgc gagcgctctc gcacggtgac
    16561 ggtcagcttc ccgctcgccg gctaggacgc cgcctgcccg cctagcgccc gcccctccag
    16621 ccaatccacc gcttcgtttt ccgtagcaaa gacccaggca ttgaacccgg ccgcgcgcaa
    16681 gtggcgctgc gcctctcgat acaccgcctc gatgggccgc gtgctcggta tcagcgccag
    16741 cttgaagtcc accggaatcc tggcgagccg cgccatggtc gcaagagcat cgcgcaacgc
    16801 gtagtgacca ttggggtggt cgtcacccgc tttcaatagc gcgcgggtga ctttgttgtc
    16861 gaggcagaaa gcagtaaagg cccggtagac cgccgaaagc tctgcggcgt tacaactgcc
    16921 gaaatcgacg cgcacgtacc ccgggcattc ggtgatgcgg ccctcgtcgc cgtagtccat
    16981 ggtcatcagc tcgccagctt cccgacgatc tcgtagcagc cgcaggaggc cgctttgagc
    17041 cccttgcgat cgagaatccg aatgttgccc cgccgatagc tgatcagctt gcgtcgctgc
    17101 agggcgccgg cggcattggt gacccccacg cgccggatgc ccagagtggc ggcgacaaat
    17161 tcgtgggtca ggtggaattg gtccgagcgc acgcggtcgc gggtcagcag cagccagccg
    17221 gcgaggcgcg cttcgacctg gtgaaaccgg ttgcacgcag cggtctgcct tgcctggccc
    17281 agcttggcat aggcatagcg gtacagctcg cgctgcaagg gcaggcagcg cgcgagcgcc
    17341 tcgcgaaagc tggccgcttt catccgcagg gcgcttcccg atccctgcac caggacacgc
    17401 acggatgaaa cttccgctcc cagaaccagc ggaattccga ccatgccttc gcggccgacc
    17461 aggccgactt ccagcgcctt gcggtccggc atcacgacca gcagcgacac ctgggcgtcg
    17521 ccgggaaagt agacgtgccg gatccgctcg ccgggctcgt acagcacctc accggagctc
    17581 agcgtgagcg gctcgagctc gccgagcaga ccctggtaat cctcgcgggg cagggctgca
    17641 agcaggctgt tggcgatcgg gccgccggcc gcggacagcg cgcgcgcggc atcgggcagc
    17701 aggaaggcgg tgcgggtctc agggtcgagc accgcgtcga cctggcccga ctcgagcgcc
    17761 tcgcgctcgg ggccgctctt gacca
//
LOCUS       DATBSZ010000104         4536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1518441, whole genome shotgun sequence.
ACCESSION   DATBSZ010000104 DATBSZ010000000
VERSION     DATBSZ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518441"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1628
                     /locus_tag="VIG70_06750"
     CDS             <1..1628
                     /locus_tag="VIG70_06750"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8250289.1"
                     /translation="RGKTVVPGLIESHTHFVSLANRPGYHVAQWELANNTAEVLATLA
                     ARRARGDVPVGAFITAMGAGTPNIWFERRMPTLAELDAAVPDRPVFLYQGGGGPARVN
                     SLGKAFFATVTNPTVTVGADGVMAGANSNAALYHLRIRQSFEDKKRSCLDAQAYTAQV
                     GVTTNLDQTLVAVATGTLQPETLDPQPNHALFNLNHYRMYDAWLALHAEGRNPTRLQI
                     NFLHNQGYIAGHDRPGQPVPDIGGQLPELRERLKNQFQFFGDDMVRTGAIGEWAAPFA
                     APTNANGYAVWLEAQRLCAKARWRNENSQAGSPTNQDAIEQVVSTYEMMHAEMIAAGF
                     PDGIKSLRWGLQHADHATVGQLERLKALNCGVSASGFRWTSAPRADGLPVGPMFPRLV
                     ASGIPLGLHQDGVHITAHNPWFAIHYATTGLNNIIPAGATQGQQINPGQQISREEALH
                     MYTRGASWYLNREDDLGSIEEGKFADLVVLDRDLFDTRRVTDAQVRAMRPILTVVDGK
                     VVHDTGALDDGGHHHHDDDDNDDDDDGGGRGKR"
     gene            1625..3352
                     /locus_tag="VIG70_06755"
     CDS             1625..3352
                     /locus_tag="VIG70_06755"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8250290.1"
                     /translation="MKTSLKLLSAALLAALALPALANGRHHDHDDDDRDTRKVKRAEA
                     LALVNGRIHTMDAKNRVVEEVLIENGRFVEVGRRVDRSGRVKVIDLRGRTVIPGIIDA
                     HNHIVLVGNRPGWHTGMEHVFTIPDAVAAYRARVAELATAGVPADEFVTTIGPISAIQ
                     FLENRLPTCDEMNSISLTRPVYIQAAQGGSRANAAAAAWFTTNAPTVVVNPTTCAVTG
                     PALQTLRQRFLTTETRKRSARDALQYFTALGITTHGDAGAFHSELPSNGIANENTYTM
                     HFPFMELQRERKLPARLRIDFLHQDSATAEPPLPTLAPRLRNSFQFFGDDWMRTQGIG
                     EFTGGGLAGLVAIGKAGWRGEDHSLSLNNFLGLVTLRQQAHDQLVAEGFPDGITKLRW
                     RISHVPGTTPESVAQWKGLGGGLLVGWGPTRGAAANGQPIGPAYRSLYDSGIPLGWHS
                     DGGDITVISPWLNFYSTITGKSLRGMHDATTPLANAGNTITRQETLRMATAANKWFTA
                     ENDLGSIEPGNHGDLVVLDRDFFDASRVPDADIPRIRSLLTVIGGQIRYDSGFFGKHK
                     GHDDDDDDD"
     gene            3339..4271
                     /locus_tag="VIG70_06760"
     CDS             3339..4271
                     /locus_tag="VIG70_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8250291.1"
                     /translation="MTTIEVEAPVRSVLICGYGLMGRGVGNTFADAGFDVKVRSSRAD
                     ALKGTRPDIAFLRELPETPPDLILELVPEDIETKRAVFREIEARYGDADFLLATGTSG
                     LDLNVLAEGLRRPERLIAIHYYMPAESIPLVEVAAGPAAPREAVDRAAEALGRTGKLP
                     LKIYKPVVGFIVNRLQHAILHEAYWMITQGIATAADVDFAAARMLGPRMCASGLILQK
                     DISGLKVNRDAQRAIVPHLFHNNEPNPLPGALFARGETGLAAGKGFYDWAGLDPAEVR
                     KTAARELDKLLAFLNRETFKNTAHLQPKPRELKP"
     gene            4306..>4536
                     /locus_tag="VIG70_06765"
     CDS             4306..>4536
                     /locus_tag="VIG70_06765"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEY8250292.1"
                     /translation="MRGRREGVAINLRRRFIDPLFEEVDASSLVALRVMFGVIMLVEC
                     WRFWSKGWIERHYIAPEFFFKYYGFEWVQAWPG"
BASE COUNT          794 a         1622 c         1497 g          623 t
ORIGIN      
        1 tgcgcggcaa gactgtggtg cccggcctga tcgagagcca cacccacttc gtgagcctgg
       61 ccaaccgtcc gggctaccac gtcgcgcagt gggagctcgc gaacaacacc gccgaggtac
      121 tggcgacgct cgcggcgcgc cgcgcgcgcg gcgacgtacc ggtgggggcc ttcatcaccg
      181 cgatgggcgc gggcacgccg aacatctggt tcgagcgccg catgcccacg ctggccgagc
      241 tcgatgccgc cgtgcccgac cggccggtgt tcctgtacca gggcggcggc gggccggcgc
      301 gcgtcaactc gctcggcaag gcgttcttcg cgaccgtgac aaacccgacg gtcaccgtcg
      361 gtgccgacgg cgtgatggcc ggcgccaatt caaacgccgc gctctaccac ctcaggatcc
      421 gccagagctt cgaggacaag aagaggagct gcctcgacgc gcaggcgtat accgcgcagg
      481 ttggcgtcac caccaatctc gatcagacgc tggtcgcggt ggcgaccggg acgctgcagc
      541 ccgagacgct cgacccgcaa ccgaaccacg cgctgttcaa cctcaaccac taccgcatgt
      601 acgacgcgtg gcttgcgctg cacgccgagg gcaggaaccc gacgcggctg cagatcaact
      661 tcctgcacaa ccagggctac atcgcgggtc acgaccggcc cggccagccg gtgccggaca
      721 tcggcggcca gctgcccgag ctgcgcgagc gcctgaagaa ccagttccag ttcttcggcg
      781 acgacatggt gcgcaccggc gcgatcggcg aatgggccgc gccgttcgcc gcgccgacca
      841 atgcgaacgg ctacgcggtg tggctggaag cgcagcgcct gtgcgccaag gcgcgctggc
      901 gcaacgagaa cagccaggcc ggtagcccga ccaaccagga cgcgatcgag caggtggtct
      961 cgacctacga gatgatgcac gccgaaatga tcgccgccgg cttccccgac ggcatcaaga
     1021 gcctgcgctg gggcctgcag cacgccgacc acgccacggt cgggcagctc gagcggctga
     1081 aggcgctgaa ctgcggcgtc tcggcctcgg gtttccgctg gaccagcgct ccgcgcgccg
     1141 acggactgcc ggtcgggccg atgttcccgc gcctcgtcgc gagcggcatc ccgctcgggc
     1201 tgcaccagga cggcgtgcac atcacggcgc ataacccgtg gttcgcgatc cactacgcga
     1261 cgaccgggct caacaacatc atccccgcgg gcgcaacgca aggccagcag atcaacccgg
     1321 gccagcagat ctcgcgcgaa gaggcgctgc acatgtatac gcgcggcgcc tcctggtacc
     1381 tgaaccgcga ggacgacctc ggctcgatcg aggaaggcaa gttcgccgac ctggtagtgc
     1441 tcgaccgcga cctcttcgac acgcgccggg tcaccgacgc ccaggtgcgc gcgatgcgcc
     1501 cgatcctcac cgtggtcgac ggcaaggtcg tgcacgacac cggcgcgctg gacgacggcg
     1561 ggcaccacca ccacgacgat gacgacaatg acgacgacga tgatggcggc ggaaggggca
     1621 aacgatgaaa accagtctga aactcctgag cgcggcactg ctcgccgcgc tcgcacttcc
     1681 ggcgctcgcc aacggccggc accatgacca cgacgacgac gaccgcgaca cgagaaaggt
     1741 caagcgcgcc gaagcgctcg cgctcgtcaa cggccgcatc cacaccatgg atgcgaagaa
     1801 ccgcgtggtc gaggaggtgt tgatcgagaa cgggcgcttc gtcgaagtcg gccgccgggt
     1861 cgaccgcagc ggccgcgtca aggtgatcga cctgcgcggc cgcaccgtca ttcccggcat
     1921 catcgacgcg cacaaccaca tcgtgctcgt cggcaaccgc ccggggtggc ataccgggat
     1981 ggagcatgtg ttcaccatcc cggacgctgt cgccgcctac cgggcgcgcg tcgccgagct
     2041 ggctaccgcc ggcgtccctg ccgacgagtt cgtcaccacc atcgggccga tctccgcgat
     2101 ccagttcctg gagaaccggc tgcccacgtg cgacgagatg aacagcatca gcctgacgcg
     2161 cccggtctac attcaggcgg cgcagggcgg cagccgcgcg aacgccgccg ccgcggcatg
     2221 gttcaccacc aacgcgccca cggtcgtcgt caatccgacc acctgcgcgg tcaccggccc
     2281 ggcgctgcag acgctgcggc agcggttcct cacgacggaa acgaggaagc gcagcgcgcg
     2341 cgatgcgctt cagtacttca cggcactcgg catcaccacg cacggcgacg ccggggcatt
     2401 ccactccgag ctgccctcga acgggatcgc gaacgagaac acgtacacga tgcacttccc
     2461 gttcatggag ctgcagcgcg agcgcaagct cccggcgcgg ctgcgcatcg acttcctgca
     2521 ccaggactcc gcgaccgccg agccgccgct gccgaccctc gcgccgcggc tgaggaactc
     2581 gttccagttc ttcggcgacg actggatgcg cacccagggc atcggcgagt tcaccggcgg
     2641 cgggctggcg ggactggtcg cgatcggcaa ggccggatgg cgcggcgagg accattcgct
     2701 cagcctcaac aacttcctcg gcctggtcac tctgcgccag caggcgcacg accagctcgt
     2761 ggccgaggga ttccccgacg gcatcaccaa gctgcgctgg cgcatctcgc acgtgccggg
     2821 caccacgccg gagtcggtgg cgcagtggaa ggggctgggc ggcggcctgc tggtgggctg
     2881 gggcccgacg cgcggcgccg cggcgaacgg ccagccgatc ggcccggcgt accgctcgct
     2941 ctacgacagc ggaattccac tgggctggca ctccgacggc ggcgacatca ccgtgatcag
     3001 cccgtggctc aacttctact ccacgatcac cgggaagagc ctgcgcggca tgcacgacgc
     3061 gacgacgccg ctggccaacg ccggcaatac gatcacgcgc caagagacgc tgcgcatggc
     3121 caccgcggcg aacaagtggt tcaccgccga gaacgacctg ggctcgatcg agcccggcaa
     3181 ccatggcgac ctggtggtgc tcgaccgcga tttcttcgat gcaagcaggg tcccggacgc
     3241 ggacatcccg cgcatccgct cgctcctgac ggtgatcggc gggcagatca ggtacgacag
     3301 cggcttcttc ggcaagcaca agggacacga cgacgacgat gacgacgatt gaggtcgaag
     3361 cgccggtccg cagcgtcctc atctgcggct acggcctcat gggccgcggc gtcggcaaca
     3421 ccttcgccga tgccggcttc gacgtcaaag tgcgctcgag ccgcgccgat gcgctgaagg
     3481 gcacgcggcc ggacatcgct ttcctgcgcg agctgcccga gacgccgccc gacctcatcc
     3541 tcgagctggt ccccgaggac atcgagacca agcgcgcggt cttccgtgag atcgaggcgc
     3601 gctatggcga cgcggacttc ctgctcgcca ccggcacctc ggggctcgac ctcaacgtgc
     3661 tcgcggaagg attgaggcgc cccgagcggc tgatcgccat ccactattac atgccggccg
     3721 agtcgatccc gctggtcgag gtggcggccg ggccggccgc accgcgcgag gcggtcgacc
     3781 gcgccgccga ggcgctcggg cgcaccggca agctgccgct gaagatctac aagccggtgg
     3841 tcggcttcat cgtcaaccgg ctgcagcacg cgatcctgca cgaggcctac tggatgatca
     3901 cgcagggcat cgccaccgcc gcggacgtcg atttcgccgc ggcgcgcatg ctcgggccgc
     3961 gcatgtgcgc gagcgggctc atcctgcaga aggacatcag cggcctgaag gtcaaccgcg
     4021 acgcgcagcg cgcgatcgtg ccgcacctct tccacaacaa cgaacccaac ccgctcccag
     4081 gcgccctgtt cgcccgtggc gagaccgggc tcgccgccgg caagggcttc tacgactggg
     4141 ccgggcttga ccccgccgag gtgcgcaaga ccgccgcgcg cgagctcgat aagcttctcg
     4201 ccttcctgaa ccgggaaacc ttcaagaaca ctgcgcacct ccagcccaag ccccgcgagc
     4261 taaaaccctg aggcccgacc ccaggggtgg gatttctggc aaaatttgag agggagacgg
     4321 gagggggttg ccatcaacct gcggcgccgg ttcatcgatc cgctgttcga ggaggttgac
     4381 gcctcttcgc tggtcgcgct gcgcgtgatg ttcggcgtca tcatgctggt ggagtgctgg
     4441 cgcttctggt cgaagggctg gatcgagcgc cactacatcg cgcccgagtt cttcttcaag
     4501 tactacggct tcgagtgggt ccaggcctgg ccgggc
//
LOCUS       DATBSZ010000105        18022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_83172, whole genome shotgun sequence.
ACCESSION   DATBSZ010000105 DATBSZ010000000
VERSION     DATBSZ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18022
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83172"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VIG70_06770"
     CDS             complement(<1..1341)
                     /locus_tag="VIG70_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY8250293.1"
                     /translation="MAVISQRLAEFTARLSLEQMPAAVRLRAKHLLLDALGCAFAARR
                     EEFATRIAASVARLAGTGPRRVIGMPHRLPLRDAALVNGMLMHGLDYDDTHAAGILHV
                     TVSTFPAALAVAAHVRASGAALLTAYIAGVETGARIAAAVKGGLHQVGFHPTGVVGAF
                     ASTLVAGRLMALSPERLAEAQGIALSFASGNLQFLEDGSWTKRIHPGWAAACGITAAA
                     LAADGFPAPREAYEGRFGLYRSHLPPDLLEKSDLSLATTGLGSVWELDNVAVKPFPAC
                     HFVHGCADAAIALHRAGVDVERVRSVRALVPEGVVKAVCEPVAAKRRPKSDYDAKFSI
                     PYAVASSLARGRLGVAEFLPAAFAEPRIERLMDQVDYAVDAQSTFPRHYTGEVVVTLD
                     DGRTLRNRVAVNRGNPDRPLTNAEIEAKYFENCALTLEESAAKRIRNAVLDLESI"
     gene            complement(1382..2107)
                     /locus_tag="VIG70_06775"
     CDS             complement(1382..2107)
                     /locus_tag="VIG70_06775"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8250294.1"
                     /translation="MKTEASPRYLRIAQDLRQAIAKGRYPVGAQLPTEHELCRQLGVS
                     RFTIREAIRVLSNAGLVRRKPRAGTLVAALPDDTRYSHGISSLRDLFQYAQSTHFHYV
                     YIGRVGLTRPQARALSAAAGEEWIHAVALRRETAGAKPFGVTRLFLNPALKGIEKALR
                     KWSGAVYALIERDFGVRIGRVEQVIAALALDEQDAASLGVPAGTPALSIRRRYFDEGG
                     RVVEMADTVHPADRFAYRMELRR"
     gene            2163..2684
                     /locus_tag="VIG70_06780"
     CDS             2163..2684
                     /locus_tag="VIG70_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY8250295.1"
                     /translation="MSGEAKRVGERTYRESFGRYFEDFRVGDIYQHRPGRTITQYDNV
                     SFTLLTMNTHPMHFDEEYAKHSEFGRCIVCSPLTVALMVGMSVTDVSQKAIANLGWTD
                     IRLTHPLFAGDTLYAESEVLDKRESASRPGAGIVAVKTVGLNQAGVKVCEFNRTMLIM
                     KRAGNDVEIRANY"
     gene            2692..3843
                     /locus_tag="VIG70_06785"
     CDS             2692..3843
                     /locus_tag="VIG70_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8250296.1"
                     /translation="MGHSDPIEEAALLDAIQRWLEKEVRPQVMRLEHDDIYPREMVET
                     MKQMGLFGAVLDPQWGGLGLSASTYAKIVMQISTVWMSLTGIFNSHLMMAMQVQKFGT
                     EEQKKRFLPRMASGELRGGLALTEPDAGTDLAAIRTRAVREGDHYVINGTKTWISNGI
                     EGGCCAVLVRTNPQTQPARKGLSMFIMEKGPGFTASRKLEKLGYKGIDSAELVFDNFK
                     VPAANLIGGEGEGFYCAVSGLELGRINVAARGAGIAKAALDESVRYAQLRKTFGKPIA
                     QHQAIQLKLGEMATRVEAARLLIEQAARAYDAGQRCDMEAGMAKYFASEAGLENALEA
                     MRIHGAYGYSKEYPIERLVRDAPLMCIGEGTNEMQRIIIARQLIERNPV"
     gene            complement(3840..4877)
                     /locus_tag="VIG70_06790"
     CDS             complement(3840..4877)
                     /locus_tag="VIG70_06790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY8250297.1"
                     /translation="MAAARSNLQPGPWSYLIGGAETETTVKRNRQALDSIAFRPRVLR
                     DVSRINSSSTFLGRKTRLPVMLAPIGGLESIVDGGAAAAARAAAQFGVPQMLSSVCQP
                     GLEQTAAAADAVRVFQLYVRGDDAWVDDFVRRAKDNGFCAFCLTVDVAHYSRRERDIV
                     GRFVKPWRARAVEGAAFQAGLSWDHVKRFKDKHDLPLIIKGIATVEDAEIAVQHGVEV
                     VYVSNHGGRQLDHGLGSLSVLPEVIKTVAGRAEVWVDGGFMRGSDVVKAIALGAKTVG
                     IGRLCGLGLAAGGPAGLVRTLELLEEEVRVCLGLLGVTSYAELTPAHLAPALPANPPD
                     TFSAFPLLTLP"
     gene            complement(4904..5239)
                     /locus_tag="VIG70_06795"
     CDS             complement(4904..5239)
                     /locus_tag="VIG70_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEY8250298.1"
                     /translation="MKIYGIRNCDTMKKAFASLEARKLAYEFHDYKKDGVPPGKLQEW
                     AKRVGWERLANTRGPTWRNIPDAEKAGLTEARALALLERYTSAIKRPVLEAGSRLLVG
                     FDPAQYDQL"
     gene            complement(5236..6408)
                     /locus_tag="VIG70_06800"
     CDS             complement(5236..6408)
                     /locus_tag="VIG70_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8250299.1"
                     /translation="MLIDFFLKLKSHKLPVSIKEYLTLLEAMGKGVISPSIDEFYYLS
                     RTALVKDEANFDKFDRAFGEFWQGVETIPGIEAQIPLEWLLKQVELHLTDEEKKQIEA
                     LGGWEKLMETLKQRLEEQKGRHQGGNKWIGTGGTSPFGAYGYNPEGVRIGQEKSRNRS
                     AVKVWDQREYRNLDDSVELGTRNIKVALRRLRKFAREGAPDEFDLQTTIEETAKKTYL
                     DIHMRPERRNAIKVLMLMDIGGTMDDHIKLAEELFSAAKTEFKHLEFYYFHNCLYDFV
                     WKDNRRRHSERTRTWELLHKYGHDYKVIFVGDATMSPYEILQPGGSVEYFNEEPGAVW
                     IKRVTDIYAKCVWLNPEPEEIWSYRQSIGIVKELMNGRMYPTTLNGLERAMKLLAK"
     gene            complement(6402..7082)
                     /locus_tag="VIG70_06805"
     CDS             complement(6402..7082)
                     /locus_tag="VIG70_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250300.1"
                     /translation="MSLAGKGVVAIWHGIRPEGRDEFYEWHNREHMPERVGIPGFLRG
                     RRYIAREGSPEFFTLYETATPEVLSGADYLARLNAPTEWTRRATLHFTDASRSLCRVV
                     LSLGPGAGGWLMTWRYDVAPGREDEQRARLEPVLKEIAQAQGVVGAHLCIADASASAI
                     QTEEKKGRPKNAVPGWVVMAEGGAEPDALHAACRRLAAPAFFTEAGALPGGQRGLYQL
                     QNAILPAC"
     gene            7154..8044
                     /locus_tag="VIG70_06810"
     CDS             7154..8044
                     /locus_tag="VIG70_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8250301.1"
                     /translation="MTGASGFIGANVVHALRARSHEVVEFSLEAGGDVTDTQLLEKRM
                     REHRIEAVWHGAAITSGAEREKREAGRILEVNTLATVRALEAAARAGVKRFVYPSSSA
                     AYGSTAFEGEGPVEEHEPLRPFNLYGISKAAAESAVLRLGPALGVEACAGRITAVFGP
                     WERDTGLRDTLSPHLQMAAMARDGREAVLARGADRDWVYAPDAAQAFVRMLEAPSLPA
                     VAMNITQGKIWPLEIMARALPGLRWRYGEPTNLGYGGPIDRPRRCLSNRLILETLGWA
                     PAYSPETACTDYVRRLPSRT"
     gene            complement(8031..8558)
                     /locus_tag="VIG70_06815"
     CDS             complement(8031..8558)
                     /locus_tag="VIG70_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEY8250302.1"
                     /translation="MPIMKKLLLIAAGALLAACQSTPDDPLRATAGLQPTKGSKAFGE
                     ATFEEVEGGKVRVIAFAQGLKPNAEHGFHIHEAGDCSSGDGMSAKGHFNPYAKPHGDP
                     KSAERHAGDLPSFKAGKDGRAKIDVTVDILTVRAGPASVVGRGLIIHADPDDYKTQPT
                     GNAGARIACGVIKSG"
     gene            8595..9692
                     /gene="dctP"
                     /locus_tag="VIG70_06820"
     CDS             8595..9692
                     /gene="dctP"
                     /locus_tag="VIG70_06820"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY8250303.1"
                     /translation="MKHLIAVACAVAALFASTAGAQAPKPVVLRFASDFTGPPHPAGM
                     AMKHFGDRLPQVIPGSEARLYFAGALYTIPEAFEAMRQGNLEMTWMQMGKAAPVEPWM
                     MAVVGPGILTTAGAVDAFDKTQTYKMLVERLEKRQAIKVFGAGQMSFGMGVGAKKRYL
                     KPEDFTGRKIRSMGPAENPVLESWKASPVVMAFGEVPTALESGVIDGLMTSIGGWLSV
                     REQSPYYTTGGAGVFTGDYYMISASRRWWDRLPKATQTALEGLISETIQLQKEFNWCV
                     DKLTYEKYGTKDPNKPGVYWMTPQEVSKLTDALGDGPSRWIKSKMPPEAAKWVDTFKQ
                     EGKTLSAQNPVGSSWIEKIDCSKHASKIVIR"
     gene            9696..10247
                     /locus_tag="VIG70_06825"
     CDS             9696..10247
                     /locus_tag="VIG70_06825"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY8250304.1"
                     /translation="MEAAYAAWRAFQDRILAPAAAILFVGSTLVALLEVGRRYILGLS
                     FEWQQDAVTYFILSGVFLYFSISQRREAHLTVTLIPELFNVFGGRWRAAAEVVKLVAL
                     VFSFAFMSAVVWWGIPEVEDAVKYESRSESLAFPMWPFLAVLLFAFASMAITMLFQIY
                     REVQALRGRTVLVEKREEGDGTH"
     gene            10247..11530
                     /locus_tag="VIG70_06830"
     CDS             10247..11530
                     /locus_tag="VIG70_06830"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY8250305.1"
                     /translation="MAIVGIVLFALLLLAVPIGIALSSAAAAFIMVDPLLETTVIFRQ
                     FFSFIGRYSLTAIPLFIFAGFLMEKTGLIRQLFAFADSLVGHLPGGFGVATVIACVLF
                     GSISGSSVAMAAAMSVIAIPEMKKRGYPDWLSGGLVATAGGIAILIPPSIPLLIYGIV
                     TETSVVRLFFAGVIPGLMLALGNILTIIVIALWLKLPRGGKFVAARALRESKGALPAL
                     GMPVVILGGLYGGLFTPTEAAAAACGYAMLYGLIVGRGQFVRELLPTAMRAMNLTGVI
                     LFLVGSVGVFQFLAANMYWPQRITDTIVGWGLTPLGFVFAYMGVLLILGMFVDGVAMI
                     LLTVPVIFPVAYAMKIDPVALGIMLTLNIEMGVVTPPVGLNLFAVSGTSRIPLHEVIK
                     GTIPFFIGDGVVLLAVIFFPGLATWLPDLLVKSVF"
     gene            11535..13376
                     /locus_tag="VIG70_06835"
     CDS             11535..13376
                     /locus_tag="VIG70_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8250306.1"
                     /translation="MIRLSQVSLRRGAKLLLEQTDLTLNPGEHAGLIGANGSGKSSLF
                     ALLRDELHADKGDVDLPGHWRIAHVAQETPALERPAVEYAIDGDARLRGLEQKLSFAE
                     SANDGSLMAELHAALADADVYTARPRAEALLLGLGFKVEELEQPVSSFSGGWRMRLNL
                     AQALMSPSDLLLLDEPTNHLDLDAIIWLEEWLRRYPGTLMVISHDRDFLDGVVKAIVH
                     IDSRKLRRYTGDYSSFEQQRAQTVLLTQNLIEKQARERAHLQSFIDRFRAKATKARQA
                     QSRMKMLAKMEDLAPLHVAAPFSFEFRDPERSPDPLLVLEDVAAGYGEKVVLKDLKLS
                     IRAGERIGLLGVNGAGKSTLVKTIAGTLSPLAGNIVFGKGLEVGYFAQHQVEMLRDDE
                     SPLWHLKRISENKIREQQLRDYLGSFNFPGDMATDPVGNFSGGEKARLSLALIVWQKP
                     NLLLLDEPTNHLDLDTREALAVALAQFEGTLVLVSHDRHLLRATTEKLLIVANGALRE
                     FDGDLDDYRDWLLKRDLVPKKEKERKPVPAKPKPASRKPNEARVKRLEELMTRLNARK
                     AEIEARLGDPGVYQDPAVLKALLLDQAYVAKELEQVEAEWLERTAGD"
     gene            13373..14533
                     /locus_tag="VIG70_06840"
     CDS             13373..14533
                     /locus_tag="VIG70_06840"
                     /inference="COORDINATES: protein motif:HMM:NF022738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 domain-containing protein"
                     /protein_id="HEY8250307.1"
                     /translation="MSRFYADLPVITEFSAVTQAERFAPLPGDWHVAACDVRNSTAAV
                     QAGNYKQVNTVAAAAVTALLNAAGDIDIPFVFEGDGSAFCVPPALLEDVRAALAKTRD
                     MARASFGLELRVATVPATRVREAGFDILVARYQVSENYVQAVFAGGGMAWAERYMKDP
                     ATAGSCAIGAGVAPRGSHEGLECRWRDIPSPHGETVSLIVRSTAGGTRVYRDLIEKVA
                     QIYGSDEQCHPLTLPALSMTLGGRRLDNEVGVRAADRDWWGRWRYRMWIRLVVVLGWF
                     LMKFGVRTEETDWTRYKPTLIRNSDVRKFNDVYRQILAGTAAQREALTAWLEERYARR
                     ELAYGLHVTDRAHMTCLVFNYSGRHLHFIDGADGGFFSAATAFKERAARLAA"
     gene            14560..15636
                     /locus_tag="VIG70_06845"
     CDS             14560..15636
                     /locus_tag="VIG70_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8250308.1"
                     /translation="MKEKSQTRRELLKRGAALAVAAPYVITARSQETLIVNTQGGEYQ
                     ELVERVVIRPFEKKFGVKVIHDATGTASQDYAKIRAARGAPGFDVAGLLTPPEVILGV
                     KEGFLEKLTEREVPNLKYLWDKTWQVIPGGSGAPHTLQYAALVYHKEKIERPRSWADY
                     WEPQKRYGDKIKGHVINYNPANLLSVYALIHGAQLGGGGVDNMDPAWARLKAQKPYVG
                     VVVTGSAEAVPHFENGQVWISPYWSGRSGYYIDRGLPFEMLIPQEGVIGLFDVACVPV
                     GAKNKKLAYEFLNWRLDKEVQRQWALAYFTSPTRSDITDLPAKFAASQIVTKEQMAKT
                     QFPDSDVIGARRKDWTLKWQEIMA"
     gene            15633..16469
                     /locus_tag="VIG70_06850"
     CDS             15633..16469
                     /locus_tag="VIG70_06850"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8250309.1"
                     /translation="MSRTPWLLVAAPMALLLVFFVVPNALLLSTSFLKSEAQTLTGEL
                     TLENYGFLFSKPIYQWAFARTFIVGASVGLLCVLLGYPLAYFLVRTKSRWKGVLIALS
                     LAPLLASVVVRTYGWYIILNRFGVANDLLLNIGLIHERIAFMPSTGAIIIGLAHALLP
                     YTVLTIMGSLNGINPNLEQAAMSLGASRTRTFFHVVLPLSLPGIAGGFILAFSIAISA
                     YATPAILGGPATQVMATAIYGFMTQLLDWSIGAALAVILIVSSLALLYAASRVGAKQA
                     AI"
     gene            16466..17269
                     /locus_tag="VIG70_06855"
     CDS             16466..17269
                     /locus_tag="VIG70_06855"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8250310.1"
                     /translation="MSPWAALRLAVVFIYLFMLGPILITAAVSFNQSNRSEFPPRGFS
                     LQWWGRALSPEWIDPLVFSLKLGLLAALISTLLAMPLAFALARHRFRGRDAMVALTLG
                     PLMLPALVTGVGLLQLFQYAGLREYIGFTALLVGHVVICLPFAVRTVAISLHTLPPNL
                     ELAAASLGASRWRTLWHVVFPLIKSGIVAGAVFAFVHSFTDVNLSLFLVRPGEVPITV
                     KILGFLEFGFAPTLAAVSVITLLLPLALVAVVEKLTGLGDFIYGERERV"
     gene            17247..>18022
                     /locus_tag="VIG70_06860"
     CDS             17247..>18022
                     /locus_tag="VIG70_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8250311.1"
                     /translation="MASASVSEAALRLAGITKRYGGVTAVDGVDLEVREGEFLTLLGP
                     SGCGKTTTLGLIAGFFPPSAGEIYLKGRPVAGLPPFKRDIGVVFQDYALFPHMSAGEN
                     VAFGLRMRNLPGNEIAQRIRQALDLVQLGGLAERRPLELSGGQRQRVALARALVIRPA
                     VLLLDEPLSNLDLKLREEMRVEIAGLQRRLGITTVFVTHDQGEALVMSDRIAVMNAGR
                     IEQLGRPAEIYERPATRFVAEFIGRMNFFRVDGKTIAIRP"
BASE COUNT         2889 a         5993 c         6053 g         3087 t
ORIGIN      
        1 gatcgactcg aggtccagca cggcgttgcg aatccgcttc gcggcacttt cctcgagcgt
       61 gagcgcgcag ttctcgaagt acttcgcctc gatctccgcg ttggtgagcg ggcggtccgg
      121 attgccgcgg ttcaccgcga cgcggttccg cagcgtgcgg ccgtcgtcca gggtcaccac
      181 cacttcgccg gtgtagtgcc gcggaaaggt ggattgcgca tcgacggcgt agtcgacctg
      241 gtccatgagc cgctcgatgc gcggctctgc gaaggccgcg ggcaggaact ccgccacccc
      301 gaggcggccg cgcgcgaggc tgctcgccac cgcgtagggg atgctgaact tcgcgtcgta
      361 gtcgctcttc ggccggcgct tggccgccac cggctcgcag accgccttga ccaccccctc
      421 gggcaccagc gcgcggaccg agcgcacgcg ctcgacgtcg acgccagcgc ggtgcagggc
      481 gatcgccgca tcggcgcagc cgtgcacgaa gtggcatgcg gggaagggct tgaccgcgac
      541 gttgtcgagc tcccagacgc ttcccaggcc cgtagtggca agggaaagat ccgatttttc
      601 cagcagatcc ggcggaagat ggctgcggta gagaccgaag cgcccttcgt aggcttcgcg
      661 cggcgccggg aagccgtcag cggcgagcgc ggcagcggtg atgccgcagg ccgcagccca
      721 tcccggatga atgcgcttgg tccacgagcc gtcctcgagg aactgcaggt taccgctcgc
      781 gaacgagagt gcgatgccct gcgcctcggc gagacgctca ggggaaagcg ccatcaggcg
      841 gccggcgacc agcgtcgacg caaaggcgcc gactacgccc gtggggtgaa agcccacctg
      901 gtgcaggccg cccttcaccg cggctgcgat gcgtgccccg gtctcgacgc cggcgatata
      961 tgcggtcagc aacgcggcgc ccgaagcgcg gacgtgggcc gcaacggcca gcgcggcggg
     1021 aaacgtgctc accgtgacgt ggagaatgcc ggccgcgtgc gtatcgtcgt agtccaggcc
     1081 gtgcatcagc atgccgttca ccagcgccgc gtcgcgcagc ggcaggcgat ggggcatgcc
     1141 gatcacccgc cgcgggcctg tgccggcaag ccgcgcgacc gaggcggcga tgcgagtcgc
     1201 gaattcctcc ctgcgggcgg cgaacgcgca gccgagggcg tcgagcagca ggtgtttggc
     1261 gcgcagccga accgccgccg gcatttgctc gagcgacagg cgcgcggtga attcggcgag
     1321 ccgctgcgag atcacagcca tcgcgtaatt gtccggacaa gaccgaagtg cgtcaagcgg
     1381 gctagcggcg cagctccatg cggtaggcga agcggtcggc gggatgcacg gtgtcggcca
     1441 tctcgaccac gcggccgcct tcgtcgaagt agcgccgccg gatcgacagc gcgggcgtgc
     1501 cggccggcac gccgaggctg gcggcatcct gctcgtccag agcgagcgcg gcgatcacct
     1561 gctcgacgcg gccgatgcgc acgccgaagt cgcgctcgat cagcgcatac accgcgccgc
     1621 tccatttgcg cagcgccttc tcgatgccct tgagcgccgg gttgaggaac aggcgcgtca
     1681 ccccgaaagg cttcgcgccg gcggtttcgc ggcgcagcgc cacggcgtgg atccactcct
     1741 cgcccgccgc ggccgagagc gcgcgcgcct gcggccgggt cagcccgact cggccgatgt
     1801 agacgtaatg gaagtgcgtg ctctgcgcat actggaacag gtcgcgcagc gagctgatgc
     1861 cgtggctgta gcgggtgtcg tcgggcaggg cggcgaccag cgtgcccgcg cgcggcttgc
     1921 ggcgcaccag gccggcgttc gacaggacgc ggatcgcctc ccggatggtg aagcgcgaca
     1981 cgccgagctg ccggcacagc tcgtgctcgg tgggcagctg cgcccccacc gggtagcgtc
     2041 ccttggcgat cgcctggcgc aggtcctgcg cgatcctcag gtaacgcggc gaggcttcgg
     2101 ttttcattct tcctcttttg tccatacaat tgtagccgcc acggccattc taggagagcc
     2161 ccatgtcagg cgaagcgaag cgggtcggcg aacgcaccta ccgggagtcc ttcggccgct
     2221 atttcgagga cttccgggtc ggcgacatct accagcaccg tcccgggcgc accatcacgc
     2281 agtacgacaa tgtttccttc acgctgctca cgatgaatac gcacccgatg cacttcgacg
     2341 aggagtacgc caagcatagc gagttcgggc gctgcatcgt gtgcagcccg ctcaccgtgg
     2401 cgctgatggt gggcatgagc gtgaccgacg tgagccagaa ggcgatcgcc aacctgggct
     2461 ggaccgacat aagactgacg cacccgctgt tcgccggcga cacgctctac gccgaatcgg
     2521 aggtgctcga caagcgcgaa tcggcgagcc ggcccggcgc cggcatcgta gccgtgaaga
     2581 ccgttggcct gaaccaggcc ggggtgaagg tgtgcgagtt caaccgtacc atgctgatca
     2641 tgaagcgcgc cggcaacgac gtcgaaatca gggcgaacta ctgaggtagc catgggccat
     2701 tccgatccaa tcgaagaagc cgcgctgctc gacgccatcc agcgctggct ggagaaggag
     2761 gtgcgtccgc aggtgatgcg cctcgagcac gacgacatct acccgcgcga gatggtcgag
     2821 accatgaagc agatgggcct gttcggcgcg gtgctcgacc cgcaatgggg cggcctgggc
     2881 ctgtcggcct cgacctacgc caagatcgtg atgcagatct ctaccgtctg gatgtcgctc
     2941 accggcatct tcaattcgca cctgatgatg gcgatgcaag tgcagaagtt cggcaccgag
     3001 gagcagaaga agcgcttcct gccgcgcatg gcgagcggcg agctgcgcgg cggcctcgcg
     3061 ctcaccgagc ccgacgccgg caccgacctc gcggcaatcc gcacgcgcgc cgtgcgcgag
     3121 ggcgaccatt acgtgatcaa cggcaccaag acctggatca gcaacggcat cgaaggcggc
     3181 tgctgcgccg tgctggtgcg caccaacccg cagacgcagc cggcgcgcaa gggcctgtcg
     3241 atgttcatca tggagaaggg cccgggattc accgcgtcgc gcaagctcga gaagctcggc
     3301 tacaagggca tcgactcggc cgagctcgtc ttcgacaact tcaaggtgcc ggcggccaac
     3361 ctgatcggcg gcgaaggcga agggttctac tgcgcggtca gcgggctcga gctcgggcgc
     3421 atcaacgtcg cggcgcgcgg cgccgggata gcgaaggcgg cgctcgatga atcggttcgt
     3481 tacgcccagc ttcgcaagac cttcggcaag ccgatcgcgc agcaccaggc gatccagctc
     3541 aagctcggcg agatggcgac gcgcgtggaa gcggcgcgcc tgctgatcga gcaggcggcg
     3601 cgcgcctacg acgcgggcca gcgctgcgac atggaggcgg gcatggccaa gtacttcgcc
     3661 tccgaagccg gcctcgagaa cgcgctcgag gcgatgcgca tccacggcgc gtacggctat
     3721 tccaaggagt acccgatcga gcgcctcgtg cgcgacgcgc ccctgatgtg catcggcgag
     3781 ggcaccaacg agatgcagcg catcatcatc gcgcgccagc tgatcgagcg gaatcccgtc
     3841 tagggcaggg tcagaagcgg aaacgcgctg aaagtgtccg gcggattcgc cggaagcgcc
     3901 ggggcgaggt gcgccggcgt gagctcggcg taggacgtta cgccgagcag gcccaagcag
     3961 acgcggacct cctcttccag caactcgagc gtgcgcacca ggccggcagg accgccggcg
     4021 gcgagcccga ggccgcaaag ccggccgatg ccgacggtct tcgcgccgag cgcgatcgcc
     4081 ttcaccacgt ccgacccccg catgaagccg ccgtcgaccc aaacctcggc gcgtccagca
     4141 acggttttga ttacttccgg gagaaccgaa aggctgccta gcccgtgatc cagttgccgg
     4201 ccgccgtggt tggagacata gaccacctcg acgccgtgct ggacggcgat ctcggcgtcc
     4261 tccacggtag cgatgccctt gatgatgagc ggaaggtcgt gtttgtcctt gaatcgcttg
     4321 acgtggtccc aggagagccc ggcctggaaa gcagcacctt cgaccgcgcg tgcgcgccag
     4381 ggcttgacga agcgtccgac gatgtcgcgc tcgcgccggc tgtagtgcgc cacgtccacc
     4441 gtgaggcaga acgcgcagaa gccgttgtcc ttcgcacggc gcacgaaatc gtccacccac
     4501 gcatcgtcgc cgcgcacgta aagctggaac acgcgcaccg cgtcggccgc ggccgcggtc
     4561 tgctcgaggc ccggctggca cacggaggac agcatctgcg gcacgccgaa ctgcgccgcg
     4621 gcgcgcgccg ccgcagccgc gccgccgtcg acgatcgact ccaggccgcc gatcggcgcc
     4681 agcatcaccg gtagccgggt tttccttcca agaaacgttg acgaggaatt gatgcggctc
     4741 acatcccgca gcacgcgcgg gcggaacgcg atcgagtcga gcgcctggcg gttgcgcttg
     4801 acggtggtct cggtctcggc gccgccaatc aggtagctcc acggccccgg ctgcaggttg
     4861 ctgcgcgccg cggccacgat ctcctggagg gtgaggaatt ctttcagagc tggtcgtact
     4921 gtgcggggtc gaagccgacc aggagccggg agccggcttc cagcaccggc cgcttgatcg
     4981 cgctcgtata cctctccagc agcgcgaggg cgcgcgcttc ggtgaggccg gccttctcgg
     5041 cgtcggggat gttgcgccac gtcggcccgc gcgtgttcgc gagcctctcc cagcccacgc
     5101 gcttcgccca ctcctgcagc ttgccgggcg gcacgccgtc cttcttgtag tcgtggaact
     5161 cgtaggcaag cttcctcgcc tcgagtgaag cgaaggcctt cttcatcgtg tcgcagttcc
     5221 ggatgccgta gatcttcact tcgccagcag cttcatcgcg cgctcgaggc cgttcagcgt
     5281 ggtcggatac atgcgcccgt tcatcagctc cttcacgatg ccgatcgact ggcggtagga
     5341 ccagatctcc tcgggctcgg ggttgagcca gacgcacttc gcgtagatgt cggtgacgcg
     5401 cttgatccac accgcgccgg gctcctcgtt gaagtactcg accgagccgc cgggctgcag
     5461 gatctcgtag gggctcatgg tcgcgtcgcc gacgaagatc accttgtaat cgtggccgta
     5521 tttgtgcagc agctcccagg tgcgcgtgcg ctcggagtgc cgccgccggt tgtccttcca
     5581 cacgaagtcg tagaggcaat tgtggaagta gtagaactcg aggtgcttga actcggtctt
     5641 tgccgcggaa aagagctcct cggcgagctt gatgtggtcg tccatcgtgc cgccgatgtc
     5701 catgagcatc agcaccttga tggcgttgcg gcgctcgggc cgcatgtgga tgtcgaggta
     5761 ggtcttcttc gccgtctcct cgatcgtggt ctggagatcg aattcgtccg gcgcgccttc
     5821 acgagcgaat tttcggagtc ttctcaaggc caccttgatg ttgcgggtgc ccagctcgac
     5881 cgagtcgtcg aggttgcgat actcgcgctg gtcccagacc ttgaccgcgc tgcggttgcg
     5941 cgatttttcc tggccgatgc gtacgccctc ggggttgtag ccgtaggcgc cgaagggcga
     6001 ggtgccgccg gtgccgatcc acttgttgcc gccctggtgg cggccctttt gctcctccag
     6061 ccgctgcttc agcgtctcca tcagcttttc ccacccgccg agcgcctcga tctgcttctt
     6121 ttcctcgtcc gtcaggtgca gctccacctg cttcagcagc cactcgagcg ggatctgcgc
     6181 ctcgatgccc ggaatggtct cgactccctg ccagaactcg ccgaacgcgc ggtcgaactt
     6241 gtcgaagttg gcctcgtcct tcaccagcgc ggtgcgcgac aggtagtaga actcgtcgat
     6301 cgaggggctg atcacgccct tccccatcgc ctcgagcagc gtcaggtact ccttgatgga
     6361 gaccggaagc ttgtgcgact tcagcttcag gaagaaatcg atcagcatgc ggggagtatc
     6421 gcattttgca gctggtacaa gccgcgctga ccgcccggaa gcgcgcccgc ttcggtgaag
     6481 aacgccggcg ccgccaggcg ccggcaggcc gcgtgcagcg catcgggctc cgcgccgccc
     6541 tccgccatca cgacccagcc cggcacggcg tttttcggcc ggcccttctt ctcctcggtt
     6601 tggatggcgc tggcgcttgc atcggcgatg cacagatgcg ctcccacgac gccttgcgcc
     6661 tgcgcaattt ccttgaggac gggctcgagc cgtgcacgct gctcgtcttc ccgccccggc
     6721 gcgacgtcgt aacgccaggt catgagccag ccgcctgcgc caggcccgag cgagagcacc
     6781 acgcggcaaa gcgagcgcga cgcgtcggtg aagtgcagcg tggcgcggcg cgtccattcc
     6841 gtgggcgcgt tcagacgcgc gaggtagtcc gcgcccgaga gaacttcagg cgtggcggtt
     6901 tcgtaaaggg tgaagaactc gggagaaccc tcgcgcgcga tgtagcgccg gccgcgcagg
     6961 aatccgggaa ttcccacgcg ctcgggcatg tgctcgcggt tgtgccactc gtagaactcg
     7021 tcgcggccct ccggccggat gccgtgccag atggccacca cgcccttgcc ggcgaggctc
     7081 acgttgtcag cttaggtccg ccctataatc ttcgtcaaat cggtttaccg agtcttctgc
     7141 tttgagaatt ctggtgacgg gcgcaagcgg gttcatcggc gccaacgtcg tccatgcgct
     7201 tcgcgcgcgc agccacgaag tcgtcgagtt ttcgctggaa gccggcggcg acgtcaccga
     7261 tacccagctg ctcgaaaagc ggatgcgcga gcatcgcatc gaggcggtgt ggcacggcgc
     7321 ggcgatcacg tcgggggcgg agcgcgagaa gcgcgaggcg ggcaggattc tcgaggtcaa
     7381 cacgctcgcg accgtccggg cgctggaggc ggcggcgcgc gcaggagtga agcggttcgt
     7441 ctatccgagt tcctccgccg cgtacggtag caccgccttc gaaggcgaag gcccggtcga
     7501 ggaacacgag ccgctgcgcc cgttcaacct ttacggcatc agcaaggcgg cggcggaaag
     7561 cgcggtgctg cgcctcggtc cggcgctcgg cgtcgaggcg tgtgccgggc ggatcacggc
     7621 ggtattcggg ccctgggaac gcgataccgg gctgcgcgac acgctcagcc cccacctgca
     7681 gatggccgcg atggcgcgcg acgggcgcga ggcggtgctc gcgcgcggcg cggatcgcga
     7741 ctgggtctat gcgccggacg cggcgcaggc gttcgtgcgc atgctggaag cgccgtcgct
     7801 gccggcagtc gcgatgaaca tcacgcaggg gaagatctgg ccgctggaga tcatggcgcg
     7861 ggcgctgccg ggcctgcgct ggcgttacgg ggagccgacc aacctgggct atggcggacc
     7921 gatcgatcgt ccccgccgtt gtctttccaa tcgcttgatt ctcgaaacgc tcggatgggc
     7981 gccagcctat tcgccggaaa ccgcctgcac cgactacgtg cgccgcctcc ctagccggac
     8041 ttgatcactc cgcaggcgat gcgcgcgccg gcgttgccgg tcggctgagt cttgtagtcg
     8101 tccggatcgg catggatgat gaggccgcgg ccgacgacgc tcgccgggcc cgcgcgcacg
     8161 gtgaggatgt ccaccgtgac gtcgatcttg gcgcgtccgt ccttgcccgc cttgaatgac
     8221 ggcaggtccc ccgcgtgacg ctcggccgat ttcggatcgc cgtgcggctt ggcgtacgga
     8281 ttgaagtggc ccttggcgct catgccgtcg cccgagctgc agtcgccggc ctcgtggatg
     8341 tggaagccgt gttcggcgtt gggcttgagc ccctgggcga acgcgatcac ccggaccttg
     8401 ccgccctcga cctcttcgaa ggtcgcctcg ccgaaggcct tgctcccctt ggtgggctga
     8461 aggcccgcag tggcgcgcag gggatcgtcc ggggtgcttt ggcatgccgc gagcagcgcg
     8521 ccggctgcga tcaacaataa cttcttcatt attggcatcc tcctcctcta gaatctactt
     8581 caggaggagg acaaatgaaa catctgatag cagtcgcgtg cgccgtggcc gcgcttttcg
     8641 cgtccaccgc cggcgcgcag gccccgaagc cggtggtgct gcgctttgcc tcggacttca
     8701 ccgggccgcc gcatccggcc ggcatggcga tgaagcactt cggcgatcgc ctgccgcagg
     8761 tgattccggg cagcgaggca aggctctact ttgccggcgc actctatacc attcccgagg
     8821 cgttcgaggc catgcgccag ggcaacctgg agatgacctg gatgcagatg gggaaggcgg
     8881 ccccggtgga gccctggatg atggcggtcg tcgggccggg cattctcacc acggccggcg
     8941 cggtcgatgc cttcgacaag acccagacct acaagatgct ggtggagcgg ctcgagaagc
     9001 gccaggccat caaggtcttc ggcgccggcc agatgagctt cggcatgggc gtcggcgcca
     9061 agaagcgcta cctcaagccc gaggacttca ccggccgaaa gatccgcagc atggggccgg
     9121 ccgagaaccc ggtgctcgag tcgtggaagg cgagcccggt ggtgatggcc ttcggcgaag
     9181 tgccgaccgc gctcgagtcg ggcgtgatcg acggcctgat gaccagcatc ggcggctggc
     9241 tcagcgtgcg cgagcagtcg ccgtactaca cgaccggcgg cgcgggcgtc ttcaccggcg
     9301 attactacat gatcagcgcg agccgccgct ggtgggaccg gctgcccaag gccacccaga
     9361 ccgcgctgga agggttgatc agcgagacga tccagctgca gaaggagttc aactggtgcg
     9421 tggacaagct cacctatgag aagtacggca ccaaggatcc gaacaagccg ggcgtctact
     9481 ggatgacgcc gcaggaagtc tcgaagctga ccgacgcgct cggcgacggc ccgagccgct
     9541 ggatcaagag caagatgccg cccgaggcgg cgaagtgggt cgacaccttc aagcaggaag
     9601 gaaagacact ctcggcgcag aacccggtcg gctcgtcctg gatcgagaag atcgactgct
     9661 ctaagcacgc ctccaagatc gtgattcgct aggcggtgga ggccgcctac gccgcctggc
     9721 gggcgttcca ggaccggatc ctggcgcccg cggcggccat tctcttcgtc ggctcgacgc
     9781 tggtcgcgct gctcgaggtc gggcggcgct atatcctcgg cctgtccttc gagtggcagc
     9841 aggatgcggt cacctacttc atcctcagtg gtgtcttcct ttatttcagc atttcgcagc
     9901 ggcgtgaggc gcacctcacc gtcacgctga tcccggagct gttcaacgtg ttcggcgggc
     9961 gctggcgcgc ggccgccgag gtggtgaagc tggtcgcgct ggtgttctcg ttcgccttca
    10021 tgtccgcggt ggtctggtgg ggaattccgg aggtcgagga cgcggtgaag tacgagagcc
    10081 gcagcgagag cctggccttt cccatgtggc ctttcctggc ggtgctgctt ttcgcctttg
    10141 catcgatggc gatcacgatg ctgttccaga tctaccgcga agtgcaggcg ctgcgcgggc
    10201 gcacggtact cgtggagaag cgcgaggaag gggacgggac acactgatgg ccatcgtcgg
    10261 catcgtgctc tttgcgctgc tcttgctcgc ggtgccgatc ggcatcgcgc tcagctccgc
    10321 ggcggcggcg ttcatcatgg tcgatccgct gctcgaaacg accgtcatct tccggcagtt
    10381 cttcagcttc atcgggcgct attcgctcac cgcgatcccg cttttcatct tcgccggctt
    10441 cctgatggag aagaccggcc tgatcaggca actgttcgct ttcgcggact cgctggtcgg
    10501 ccacctgccg ggcggcttcg gcgtcgccac cgtgatcgcg tgcgtgctgt tcggctccat
    10561 ctccggctcg agcgtggcga tggcggcggc gatgagcgtg atcgcgattc ccgaaatgaa
    10621 gaagcgcggc tatcccgact ggctttccgg gggcctcgtg gcgaccgcgg gcggaatcgc
    10681 catcctgata ccgccgagca ttccgctcct catctacggc attgtcaccg aaacctcggt
    10741 ggtgcggctg ttcttcgctg gcgtcattcc aggcctgatg ctcgcgctcg gcaacatcct
    10801 caccatcatc gtgatcgcgc tgtggctcaa gctcccacgt ggcggcaagt tcgtagcggc
    10861 tcgtgcgctg cgcgagtcca agggcgcgct gcccgcgctc ggcatgccgg tggtgatcct
    10921 cggcgggctc tacggcggcc tgttcacacc gaccgaggcc gcggcggccg cttgcggcta
    10981 cgcgatgctc tacgggttga tcgtcggccg cggccagttc gtcagggaac tgctgccgac
    11041 cgcgatgcgc gcgatgaacc tcaccggcgt gatcctgttc ctcgtcggca gcgtcggcgt
    11101 gttccagttc ctcgccgcca acatgtactg gccgcagcgc atcaccgaca ccatcgtcgg
    11161 ctggggactg accccgctcg gcttcgtctt cgcctacatg ggcgtgctgc tcatcctggg
    11221 aatgttcgtc gacggcgtgg ccatgatcct gctcaccgtg ccggtgatct tcccggtggc
    11281 ctacgccatg aagatcgacc cggtggcgct cggcatcatg ctcaccctca atatcgaaat
    11341 gggcgtggtg acgcccccgg tcggcctgaa tctcttcgcc gtgagcggca cctccaggat
    11401 tcccctgcac gaggtgatca aggggacgat tccgtttttc atcggcgacg gcgtcgtcct
    11461 gttggcggtg attttcttcc ccggcctcgc cacctggctg cccgacctgc tggtgaaatc
    11521 cgtcttctag cgctttgatt cgtctttcgc aggtcagcct caggcgcggc gcgaagctgc
    11581 tgctagaaca aaccgatctg acgctcaacc cgggcgagca tgcggggctc atcggcgcca
    11641 acggctccgg caagtcgagc ctgttcgcgc tgctgcgcga tgagctgcac gccgacaagg
    11701 gagacgtcga cctgccgggc cactggcgca tcgcgcacgt cgcgcaggag accccggcgc
    11761 tggagcgccc cgccgtcgag tacgccatcg acggcgatgc caggcttcgt ggtttggaac
    11821 aaaaactttc atttgcggaa tcggcgaatg acggcagctt gatggcggag ctgcacgcgg
    11881 cgctcgccga cgccgacgtc tacaccgcgc ggccgcgcgc ggaggccctg ctgctcggcc
    11941 tcgggttcaa ggtcgaggag ctggaacagc cggtgtcgag cttttcgggc ggctggcgca
    12001 tgcggctcaa cctggcgcag gcgctgatgt cgccctcgga cctcttgctg ctcgacgagc
    12061 cgaccaacca cctcgacctc gatgcgatca tctggctcga ggagtggctg cggcgctacc
    12121 cggggacgct gatggtgatc tcgcacgacc gcgatttcct cgacggggtg gtgaaggcca
    12181 tcgtgcacat cgacagccgc aagctgcggc gctacacggg cgactactcg agcttcgagc
    12241 agcagcgcgc gcagaccgtc cttcttactc aaaatttgat tgaaaaacag gcacgtgaac
    12301 gtgcgcattt gcagtcgttc atcgaccgct tccgcgccaa ggcgaccaag gcgcgccagg
    12361 cgcagagccg catgaaaatg ctggcgaaga tggaggacct ggcgccgctg cacgtcgccg
    12421 cgccgttttc cttcgagttc cgcgacccgg agcgctcgcc cgatccgttg ctggtgctgg
    12481 aagacgtcgc cgccgggtat ggggaaaaag tcgtattgaa ggacctgaaa ctctcgattc
    12541 gtgcgggcga gcgcatcggc cttctcggcg taaacggcgc cggcaagtcc acgctggtga
    12601 agaccatcgc cggcacgctt tcgccacttg ccggcaatat tgttttcggc aaaggcctgg
    12661 aggtcgggta cttcgcgcag caccaggtag agatgctgcg cgacgacgag tcgccgctct
    12721 ggcatctaaa acgaatttca gaaaacaaga ttcgtgaaca gcagctgcgc gactatctgg
    12781 gcagcttcaa tttcccgggc gacatggcga ccgacccggt ggggaatttt tccggcggcg
    12841 agaaggccag gctgtcgctc gcgctcatcg tctggcagaa gccgaacctg ctgctgctcg
    12901 acgagccgac caaccacctg gacctcgaca cccgcgaagc gctcgcggtc gccctggcgc
    12961 agttcgaggg cacgctggtg ctcgtgtcgc acgaccgcca tctgctgcgc gccacgaccg
    13021 agaagctcct gatcgtcgcg aacggcgcgc tgcgtgaatt cgacggcgac ctggacgact
    13081 accgcgactg gctcctgaag agagacctgg tcccgaagaa agaaaaggag aggaaaccgg
    13141 ttcccgccaa gcccaagccg gccagtcgca agccgaacga agcgcgcgtc aagcgcctcg
    13201 aggagctgat gacccgcctc aacgcccgca aggccgagat cgaggcacgg ctcggcgacc
    13261 cgggggttta ccaggacccg gcggtgctca aggcgcttct gctcgatcag gcctacgtcg
    13321 ccaaggagct cgagcaggtc gaagccgagt ggctggaacg gaccgccggc gattgagccg
    13381 tttctacgcc gacctgccgg tgatcaccga gttcagcgcc gtgacgcagg ccgagcgctt
    13441 cgcgccgctg ccgggggact ggcatgtggc ggcgtgcgac gtgcgcaact cgaccgcggc
    13501 ggttcaggcc ggcaattaca agcaagtcaa taccgtcgcc gcggccgcgg tcaccgcgtt
    13561 gctcaacgcc gcgggggaca tcgacatccc gttcgtgttc gagggcgacg gcagcgcgtt
    13621 ctgcgtgccg ccggcgctgc tcgaggacgt gcgcgcggcg ctcgcgaaga cacgcgacat
    13681 ggcgcgcgcc tcgttcgggc tcgagttgcg cgtggccaca gtgccggcga cccgcgtgcg
    13741 cgaagcgggc ttcgacatcc tggtggcgcg ctatcaggtg tcggagaact acgtccaggc
    13801 ggtgttcgcc ggcggcggca tggcgtgggc cgagcgctac atgaaggatc cggccaccgc
    13861 cggctcgtgc gcgatcggcg ccggcgtcgc gccgcgcggc agccacgaag ggctggaatg
    13921 ccgctggcgc gacatcccga gcccccatgg cgagacggtg agcctcatcg tgcgctcgac
    13981 ggccggggga acgcgggtct accgcgacct gatcgagaag gtggcgcaga tctacggcag
    14041 cgacgagcag tgccacccgc tgacgctgcc cgcgctgtcg atgacgctcg gcggccggcg
    14101 cctcgacaac gaggtcggcg tccgcgcagc ggaccgggat tggtggggcc gctggcgcta
    14161 ccgcatgtgg atccgcctgg tggtggtgct cggctggttc ctgatgaagt tcggcgtgcg
    14221 caccgaggag accgactgga ctcgctacaa gccgacgctg atccggaaca gcgacgtgcg
    14281 caagttcaac gacgtctacc gccagatcct cgccggcacc gcggcgcagc gcgaggcgct
    14341 caccgcatgg ctcgaggagc gctacgcgcg gcgggagctc gcctacgggc tgcacgtcac
    14401 cgaccgcgcg cacatgacct gcctggtgtt caactattcg ggcaggcacc tgcatttcat
    14461 cgacggcgcc gacggcggct tcttctccgc cgcaacagcc ttcaaggagc gcgcggcgcg
    14521 gctcgccgcg tgagttatgc ttcgcgctga ggaggagcta tgaaggagaa atcgcagacg
    14581 cgcagagagt tactcaagcg gggcgcggca ctcgcggtcg cggcgcccta cgtgatcacc
    14641 gcacgctccc aggaaacgct gatcgtcaac acgcagggcg gcgaatacca ggagctggtc
    14701 gagcgcgtgg tgatccgtcc gttcgagaag aagttcggcg tcaaggtgat ccacgacgcg
    14761 accggcaccg cgtcgcagga ctacgccaag atccgcgccg cgcgcggcgc gcccgggttc
    14821 gacgtcgcgg ggctgctcac gccgcccgag gtgatcctcg gggtgaagga gggcttcctc
    14881 gagaagctca ccgagcgcga ggtgccgaac ctgaaatacc tgtgggacaa gacctggcag
    14941 gtgatccccg gcggctcggg cgcgccgcac acgctgcaat acgcggcgct cgtctaccac
    15001 aaggaaaaga tcgagcggcc ccgctcctgg gccgactact gggagccgca gaagcggtac
    15061 ggcgacaaga tcaagggcca cgtcatcaac tacaaccccg cgaacctgct ctcggtgtac
    15121 gcgctgatcc acggcgcgca gctcggcggc ggcggggtcg acaacatgga ccccgcctgg
    15181 gcgcggctca aggcgcagaa gccgtacgtc ggcgtggtgg tcaccggctc ggccgaggcg
    15241 gtgccgcact tcgagaacgg ccaggtctgg atctcgccgt actggagcgg gcgctcgggc
    15301 tactacatcg accgcggcct gccgttcgaa atgctcattc cccaggaggg cgtgatcggc
    15361 ctgttcgacg tcgcctgcgt gccggtcggt gcgaagaaca agaagctcgc ctacgagttc
    15421 ctcaactggc gcctcgacaa ggaagtccag cgccaatggg cgctcgcata cttcaccagc
    15481 ccgacgcgca gcgacatcac cgacctgccg gccaagttcg ccgcaagcca gatcgtgacc
    15541 aaggagcaga tggccaagac ccagttcccg gactcggacg tgatcggcgc ccggcgcaag
    15601 gactggacct tgaagtggca ggaaatcatg gcgtgagccg tacgccgtgg ttgctggtgg
    15661 cggcgccgat ggcgctgctg ctggtgttct tcgtcgtccc gaacgcgctg ctgctctcga
    15721 ccagcttcct caagtcggag gcgcagaccc tcactggcga gctgaccctc gagaactacg
    15781 gcttcctttt cagcaagccg atctatcagt gggctttcgc gcgcaccttc atcgtcggcg
    15841 cgagcgtcgg cctgctgtgc gtcctgctcg gctacccgct cgcgtacttt ttggtgcgca
    15901 cgaagagccg ctggaaaggc gtgctgatcg cgctgtcgct cgcgccgctg ctggcgagcg
    15961 tcgtggtacg cacctacggc tggtacatca tcctgaaccg cttcggcgta gcgaatgatt
    16021 tgctcctgaa tatagggctc attcacgagc ggatcgcttt catgccgagc accggcgcga
    16081 tcatcatcgg gctcgcccat gcgctgctgc cgtatacggt gctcaccatc atgggctcgc
    16141 tcaacggcat caaccccaac ctcgagcagg cggcgatgag cctcggcgcg agccgcacgc
    16201 gcacgttctt ccacgtcgtg ctgccgctca gcctgccggg catcgccggc ggattcatcc
    16261 tcgcgttctc gatcgccatc agcgcctatg ccacgcccgc catcctcggc ggcccggcga
    16321 cgcaggtgat ggcgaccgcg atctacggct tcatgaccca gctcctcgac tggtcgatcg
    16381 gcgccgcgct cgcggtcatc ctgatcgttt cctcactggc tcttctttat gccgcctccc
    16441 gggtgggcgc gaagcaggct gcgatatgag cccgtgggct gcgcttcgcc ttgctgttgt
    16501 ctttatctat ctcttcatgc ttggtccgat cctcattacg gcggccgttt cgttcaatca
    16561 gtccaaccgc tcggaattcc cgccgcgcgg cttctcgctg cagtggtggg gccgggcgct
    16621 ttcgccggag tggatcgatc cgctggtgtt cagcctgaag ctcgggctcc tggcggccct
    16681 tataagcacg ctgctcgcga tgccgcttgc gttcgctctc gcgcgccacc gctttcgcgg
    16741 gcgcgacgcg atggtcgcat tgacgctcgg gccgctcatg ctgccggcgc tggttaccgg
    16801 cgtcggcctg ctgcagctct tccagtacgc cgggctgcgc gaatacatcg gcttcacggc
    16861 gctcctcgtc ggccacgtgg tgatctgtct gccgttcgcg gtgcgcacgg tggccatcag
    16921 cctgcacacc ctgccgccga acctcgagct tgccgccgcg agcctcggcg cgtcgcgctg
    16981 gcgcacgctg tggcacgtgg tgtttccgct gatcaagagc ggcatcgtcg ccggcgcggt
    17041 gttcgctttc gtgcactcgt ttaccgacgt caacctgtcg ctgttcctgg tgcggccggg
    17101 ggaggtgccg atcacggtca agatcctcgg cttcctcgag ttcggcttcg cgccgacgct
    17161 cgccgccgtg tcggtgatca cgctcctcct gccgctcgcg cttgtcgcgg tggtggagaa
    17221 actcaccggg ctgggcgact tcatctatgg cgagcgcgag cgtgtctgag gccgcgctca
    17281 ggctcgccgg catcaccaag cgctacggcg gcgtgactgc ggtcgacggc gtcgatctcg
    17341 aggtgcgcga gggcgagttc ctgacactcc ttggcccttc gggctgcggc aagaccacga
    17401 cgctcggcct gatcgccggg ttctttccgc cgagcgcggg cgagatctac ctcaagggac
    17461 gtccggtcgc cgggctgccg ccgttcaagc gcgatatcgg cgtcgtgttc caggattacg
    17521 cgctgttccc gcacatgagc gccggggaga acgtggcgtt tggcctgcgg atgagaaatc
    17581 tcccgggaaa cgagatcgca caacggataa gacaagccct cgatctggtc cagctcggcg
    17641 gcctcgcgga acgccgcccg ctcgagctct cgggagggca gcgccagcgc gtggcgctcg
    17701 cccgggcgct ggtgatccgc cccgctgtgc tcctgctcga cgagcccctt tccaacctcg
    17761 acctcaagct gcgcgaagag atgcgcgtcg agatcgccgg cctgcagcgg cgcctcggca
    17821 tcaccacggt gttcgtcact cacgaccagg gcgaggcgct ggtgatgtcc gaccgcatcg
    17881 cggtgatgaa tgcgggccgc atcgagcagc tcggccgccc ggccgagatc tacgaaagac
    17941 cggcgacgcg cttcgtcgcg gagttcatcg gtcgcatgaa cttcttccgg gtcgacggca
    18001 agacgatcgc gatccgcccg ga
//
LOCUS       DATBSZ010000106         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2337952, whole genome shotgun sequence.
ACCESSION   DATBSZ010000106 DATBSZ010000000
VERSION     DATBSZ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2337952"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /gene="xdhB"
                     /locus_tag="VIG70_06865"
     CDS             <1..1677
                     /gene="xdhB"
                     /locus_tag="VIG70_06865"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HEY8250312.1"
                     /translation="PGEHGAMTIYSSTQHPTEIQHLAAAALGIPDAAVTVELRRLGGG
                     FGGKETQAAAYAMAAALLARATGRPVKLRVDRDDDIIGTGKRHDFLYELDVGFDGEGR
                     IAGLDLMLASRCGLSCDLSNAVNDRAVLHCDNAYFLPALKVVSHRCKTNTVSATAFRG
                     FGGPQGMFAIEGAIAAIARRLGIDPLDVRKRNLYGDAPRNVTHYGMTLKDNVLPELIA
                     ELELRSDYRRRRKEITRWNAANPWFKRGIALTPVKFGIAYTNVPMNQGGALVHIYTDG
                     SVHLNHGGTEMGQGLMIKVAQIVADELEVDLSRVRITPTSTGKVPNTSPTAASSGTDI
                     NGEAARVACRVLKERLAPFRAKHPDHSLAQLARAAYNARVSLSATGYWSTPEIHFDRA
                     SFTGEPFYYFAYGAAVSEVVIDTLTGESRLLRADLLHDVGRSVNPAIDRGQVEGGFIQ
                     GMGWLTMEEVVWDAKGTLLTHAPSTYKIPVASDCPPVFNTWLWERGENVRDIVYRSKA
                     VGEPPLMLALSVFHAITDAVGSTGASRDPVPLDAPATPESILKTICALRS"
     gene            1659..>2306
                     /gene="xdhC"
                     /locus_tag="VIG70_06870"
     CDS             1659..>2306
                     /gene="xdhC"
                     /locus_tag="VIG70_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HEY8250313.1"
                     /translation="MRIEILIVETRGSAPREAGTRMWVGATEIAGSIGGGNLEYTALK
                     IAREMLLSSEMQRERKFALGDSLGQCCGGSVTLRFTKVEEMSASPEPDFDVVLFGAGH
                     VGKEVARILERLPCRLTWIDPRPEAFPSSVGKDVKVVVEEEPAWMVDEAPPGALYLVM
                     THSHALDLEIVERVLKRKDVAFLGLIGSETKAAKFRLRLQQRGVSAEGLVCPVGLF"
BASE COUNT          400 a          807 c          742 g          357 t
ORIGIN      
        1 cccggcgagc acggcgcaat gacgatctac tcgtcgaccc agcatcccac cgagattcag
       61 cacctcgccg ccgccgcgct cgggatcccc gacgccgcgg tcacggtcga gctgcgccgg
      121 ctgggcgggg gcttcggcgg caaggaaacg caggccgccg cctatgcgat ggcggccgcc
      181 ctgctcgcgc gcgccactgg ccggccggtg aagctgcgcg tggaccgcga cgacgacatc
      241 atcggcaccg gcaagcgcca cgatttcctc tacgagctcg acgtcggctt cgacggcgag
      301 gggcgcatcg cgggactcga cctgatgctc gcctcgcgct gcgggctgtc gtgcgacctt
      361 tccaatgcgg tcaacgaccg cgcggtgctc cattgcgaca acgcctactt cctgccggcg
      421 ctgaaggtcg tctcgcaccg gtgcaagacc aacaccgtat cggccacggc gttccgcggc
      481 ttcggcggcc cacagggcat gttcgcgatc gagggcgcga tcgccgccat cgcacggcgc
      541 ctgggcatcg atccgttaga cgtgcgaaag cgcaatctct acggcgacgc gccgcgcaac
      601 gtcacgcact acggcatgac gctgaaggac aacgtcctgc ccgagctgat cgccgagctt
      661 gaattgcgct ccgactaccg caggcgcagg aaggaaatca ctcgctggaa tgcagccaat
      721 ccgtggttca aaagagggat tgccctgacg ccggtcaagt tcggcatcgc ctatacgaac
      781 gtgccgatga accagggcgg cgccctggtg catatctaca ccgacggcag cgtgcacctg
      841 aaccacggcg gcaccgagat gggacagggg ctgatgatca aggtggcgca gatcgtggcc
      901 gacgagctcg aggtcgacct ctcgcgcgta cgcatcaccc cgacctcgac cggcaaggtg
      961 ccgaacacct cgcccaccgc agcctcgagc ggcaccgaca tcaacggcga ggcggcgcgc
     1021 gtcgcctgcc gcgtgctgaa agagcgcctc gcgccgtttc gcgccaagca ccccgatcac
     1081 agcctcgcgc agctcgcccg cgcggcgtac aacgcgcgcg tgtcgctgtc ggccaccggc
     1141 tactggagca cgcccgagat ccatttcgac cgcgcgagct tcaccggcga gccgttctac
     1201 tacttcgcgt acggcgccgc cgtgtcggag gtcgtgatcg atacgctgac cggcgaatcg
     1261 cgcctgctgc gcgccgacct gctgcacgac gtcggccgct cggtgaaccc ggcgatcgac
     1321 cgcggccagg tcgaaggcgg cttcatccag ggcatgggct ggctcacgat ggaagaagtc
     1381 gtctgggacg ccaagggcac gctcctcacc cacgcgcctt cgacgtacaa gatcccggtg
     1441 gcgagcgatt gcccgccggt cttcaacacc tggctgtggg agcgcggcga gaacgtgcgc
     1501 gacatcgtct accgctccaa ggcggtcggc gagccgccgc tcatgctcgc gctctcggtg
     1561 ttccacgcga tcaccgatgc cgtgggctcg accggcgctt cgcgcgatcc cgtgccgctc
     1621 gacgcgccgg ccacgcctga gagcatcctg aagacgatat gcgcattgag atcctgatcg
     1681 tcgagacccg cggctcggcg ccgcgcgagg ccggcacacg catgtgggtc ggcgcgaccg
     1741 agatcgccgg ctcgatcggc ggcggcaacc tcgaatacac cgcgctcaag atcgcgcgcg
     1801 agatgcttct gtcgtccgaa atgcaaaggg aacgcaagtt cgcgctcggc gacagcctcg
     1861 gccagtgctg cggcggcagc gtaacgctgc ggttcacgaa ggtggaagag atgagcgctt
     1921 cgcccgaacc cgatttcgac gtcgtactct tcggcgccgg ccacgtcggc aaggaagtcg
     1981 cgcgcatcct cgagcggctg ccgtgccggc tgacctggat cgatcctcgc cccgaggctt
     2041 tcccatcatc agtcggaaag gacgtgaaag tcgttgtaga ggaagagcca gcctggatgg
     2101 tcgacgaagc gccgcccggc gcgctctatc tcgtcatgac ccacagccat gcgctcgatc
     2161 tcgaaatcgt cgagcgcgtc ctcaagcgca aggacgtcgc gtttctcggc ctgatcggct
     2221 cggaaacgaa ggccgcgaaa ttccgccttc gccttcaaca acgaggagtt tctgcggaag
     2281 gcctggtttg ccccgtcggc cttttc
//
LOCUS       DATBSZ010000107        28102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_698336, whole genome shotgun sequence.
ACCESSION   DATBSZ010000107 DATBSZ010000000
VERSION     DATBSZ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 28102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28102
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698336"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..513
                     /locus_tag="VIG70_06875"
     CDS             145..513
                     /locus_tag="VIG70_06875"
                     /inference="COORDINATES: protein motif:HMM:NF016050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HEY8250314.1"
                     /translation="MKIRISAAGASLEAVLKDTPTAKALVAAMPFEARAQTWGEEVYF
                     ITPVSARLEPDAKQVVEPGTVCFWTEGDALALPYGRTPISTDARPKLANPCNLVGTLV
                     GDPKALSRIKAGEKITVSKA"
     gene            513..722
                     /locus_tag="VIG70_06880"
     CDS             513..722
                     /locus_tag="VIG70_06880"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEY8250315.1"
                     /translation="MQRKRVNSSKLRSVGYDEPTRTLEIEMSNGEVFQYGGVYPEVYR
                     RLMAAPNPTSFYDDKIADEYTAKRV"
     gene            765..1262
                     /locus_tag="VIG70_06885"
     CDS             765..1262
                     /locus_tag="VIG70_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250316.1"
                     /translation="MRRGLIMAMLGLACAAAQAATPQRELAQPAPVFAFDSLHGIVSW
                     LRQHGREGYIGADVADAMGIPRPEDEPLVAAWQRGFKNDDVLRIAQVLDNPAADGERD
                     FILFMVQRPDDQVYFYLSTPLGGLQKAFLSIPSKNLVMPLERAEAELRFREEVLYWKD
                     RSEAR"
     gene            1262..2366
                     /gene="prfB"
                     /locus_tag="VIG70_06890"
     CDS             join(1262..1342,1344..2366)
                     /gene="prfB"
                     /locus_tag="VIG70_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417196.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY8250317.1"
                     /translation="MEAERLNQIASQLTDLQARSAELRRYLDLEGKQKRLTEVERELS
                     DPQVWSDQQRAQELGKEKKALESVVGTLGRVGSGLSDAAELFGMARAESDDATLAGVA
                     SDVEKIEKDVAAMEFRRMFSNPLDPNNCFIDIQAGSGGTEAQDWAAMLERMYLKFCDR
                     RGFSAEVLEHTPGEVAGIKSASLKVSGDYAYGTLRTETGVHRLVRKSPFDANARRHTS
                     FASVFVYPEVDESIEVEVNPADLRIDTYRASGAGGQHVNRTDSAVRITHLPTNTVVQC
                     QNDRSQHRNRAEAMAMLKAKLYELEIRKRLAEKQKLEDAKTDIGWGHQIRSYVLDQSR
                     IKDLRTGVEKGATQAVLDGDLDDFITASLKQGV"
     gene            2368..3819
                     /gene="lysS"
                     /locus_tag="VIG70_06895"
     CDS             2368..3819
                     /gene="lysS"
                     /locus_tag="VIG70_06895"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY8250318.1"
                     /translation="MEEDNKLVDERREKLKALRSAGPAYPNDFRREHLAGDLHASHGN
                     KIKEALEKAVIPVKVAGRMMLKRVMGKASFATLQDSSGRIQIYVSNEVENYEAFKHWD
                     LGDILGVEGLLFKTMKGELTVSARKIRLLAKALRPLPEKFHGLADHEMRYRQRYVDLI
                     VNPQVKDVFFKRTRIIQTLREVLAADGYVEVETPMMQPIPGGAAARPFKTHHNALDME
                     LYLRIAPELYLKRLVVGGLERVFEINRNFRNEGMSTRHNPEFTMLEWYCAYQDWRYMM
                     EHTEKLVRAAAGGAVVTYQGKQIDFGKPFARITMPDAIRKQGITGDLRDRAFLSKSLT
                     AMKIEHEKHHGWGALQLMLFEAVAEKHLVEPTFVTDFPAEVSPLSRRRDGDPEVADRF
                     ELFIDAKEIANGFSELNDPEDQAQRFLEQVKKKNAGDDEAMHYDADYIRALEYGMPPC
                     AGGGLGIDRLVMLLTDSPSIRDVILFPQMRPEV"
     gene            3921..4532
                     /locus_tag="VIG70_06900"
     CDS             3921..4532
                     /locus_tag="VIG70_06900"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY8250319.1"
                     /translation="MQATASRLHPLLTLAAISVTVLSAVGVASLTGMIPSSKSQENTL
                     QLPSEVLKPIEPAISHPVTKPAARKIVARKAAPQPVEPTVLVSETPKSQPLVGQLAVV
                     ESVREVKEPGDAKGVGAVAGGVVGGVLGNKLGKGKGLVTILGAAGGAFAGHQIEKQAR
                     AEKRWEIALRLDDGSQRTLSSDAEPAWRTGDRVRLVDDRLERI"
     gene            complement(4529..5437)
                     /gene="rssA"
                     /locus_tag="VIG70_06905"
     CDS             complement(4529..5437)
                     /gene="rssA"
                     /locus_tag="VIG70_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HEY8250320.1"
                     /translation="MPRKLRLGLALGAGSSRGWAHIGVIRALEERGIKPDLVCGTSIG
                     ALVGAAYAGGELERLEQWVTGLAWTTVVRLMDLTWRGGLIRGTRLFTLFRTILQDRDI
                     SELPIAYGAVATELQSGRELWLRTGKVLDAVRASCAMPGLLTPVIRDGVVLVDGGLVN
                     PVPVSMCRALGADLVVAVDLSWGKLGPYRRTKEREVAPREEPSWLGRFVPGKAQRKGK
                     PDNPSIVSQDEPSIPSIFNVFLTSLDIVEMRVARSRLAGEPADVLITPLLPDFATMDF
                     HRAGEAIAEGRAAVDRMGPLLKQVLG"
     gene            5590..5853
                     /locus_tag="VIG70_06910"
     CDS             5590..5853
                     /locus_tag="VIG70_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250321.1"
                     /translation="MRQSRIETACAEIRRALLERRRAIAEEIARHPRPITACDVHFNR
                     MLEERASISQEIDRFDAFISTSELLTPAAKARVLVSEENVVCP"
     gene            complement(5863..7044)
                     /locus_tag="VIG70_06915"
     CDS             complement(5863..7044)
                     /locus_tag="VIG70_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HEY8250322.1"
                     /translation="MSNQVVVVSGVRTGIGDYGGALKELAATKLGAVAIKEAVARAKI
                     EPARVGHVVMGSVIHGEAKDMYLSRVAAIEAGIPVGTPCLTVNRLCGSGLQAIVSAAQ
                     HLMIGDCDVAVAGGAESMSRAAYFLPAGRWGQRMGDATVVDAMTGALHDPFGHGHMGV
                     TAENIAAKYGFTRQQQDEFSISSHQKAARAIEAGHFKSQIVPIELKTRKGVEQFTSDE
                     HVRKDIKPEDMAKLKTVFKKDGTVTAGNASGINDAGAAVVLTSSSFAEKNNLKPMARL
                     VAYAHAGVDPQVMGLGPIPAVKRVFEKAGLKPADMDVVESNEAFAVQAMAVTKDLGLD
                     PARVNPNGGAVALGHPIGATGCILTVKALYELERTQKRYALVTMCIGGGQGIAAIFER
                     L"
     gene            complement(7041..8342)
                     /gene="rimO"
                     /locus_tag="VIG70_06920"
     CDS             complement(7041..8342)
                     /gene="rimO"
                     /locus_tag="VIG70_06920"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY8250323.1"
                     /translation="MLKSSSRTKAPTVGFVSLGCPKALVDSERILTQLRAEGYETSKT
                     YADADLVIVNTCGFIDAAVEESLGAIGEALAENGKVIVTGCLGVKNDFIRAKHPAVLA
                     ITGPHDTPAVMSAVHAHLPKPHDPYTDLIGVKLTPPHYAYLKIAEGCNHRCTFCIIPS
                     MRGDLVSRPIGEVLQEAENLVRGGVKELLVISQDTSAYGVDVKYRTGFWQGRPVKTRM
                     TQLCESLGQLGAWVRLHYVYPYPHVDEVIPMMAEGKILPYLDVPFQHASPRILKLMKR
                     PANAEKVLERIRGWRAVCPDITLRSTFIVGFPGETEDDFSELLSFLKEAQLDRVGCFE
                     YSPVEGAAANALPDPLSPEVKRYRKEQFMKTQEAISASKLKRKVGRTVKVLVDEPGVG
                     RSSADAPEIDGVVRFKGGKAGEFRDVTIDRAGEHDLYGRLQ"
     gene            complement(8326..8892)
                     /gene="phaR"
                     /locus_tag="VIG70_06925"
     CDS             complement(8326..8892)
                     /gene="phaR"
                     /locus_tag="VIG70_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY8250324.1"
                     /translation="MENQLRLIKKYPNRRLYDTKTSSYITLADVKQMVLKQEEFQVVD
                     AKSGEDLTRQILLQIILEEESGGVPMFSSELLSQLIRFYGHAMQGLMGTYLEKNIRAF
                     HDIQKTLQEQSTRMYGDNSRASQELWAQFMNLQGPAMQSLMQAYMEQSQKMFAQFQDQ
                     MQSQARNMFSGLSFPGFSQTGKSDPEKQ"
     gene            complement(8951..9697)
                     /gene="phbB"
                     /locus_tag="VIG70_06930"
     CDS             complement(8951..9697)
                     /gene="phbB"
                     /locus_tag="VIG70_06930"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEY8250325.1"
                     /translation="MAKQRVAVVTGGMGGLGETISLKMAGAGYRVVVTYSPSNTKYKS
                     WLEEMKGKGHSFAAFPVDVVDYDDCARKIAAIQAEVGPVDILVNNAGITRDMTFKKMS
                     KADWDAVMRTNLDSCFSMTKQVMDGMVDRGWGRVINVSSVNGQKGAFGQTNYSAAKAG
                     IHGFTKALALEVARKGVTVNTISPGYIGTKMVTAIPKEILDSKILPQIPIGRLGKPDE
                     VAGLIIYLCSEEAAFVTGANISINGGQHMY"
     gene            complement(9736..10536)
                     /locus_tag="VIG70_06935"
     CDS             complement(9736..10536)
                     /locus_tag="VIG70_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HEY8250326.1"
                     /translation="MGNQAEVVQRPETWRDESSPVVSRIAFVSGGMGGIGSAICRRLG
                     RAGHTVVAGCLPGYEKKTEWLDAMRALGFRVHAAEGDVSEFESCAEMFYNVRSVVGPI
                     DILINNAGITRDSVFKRMTEQDWMAVINTNLNSVFNVTRQVIDGMSERGWGRIINISS
                     VNAIKGQFGQTNYSAAKAGMAGFSKALAQEVVRKGVTVNTVSPGYVETDMVMAIRQEI
                     RDQIVASIPMGRLAKPEEIAAVVAFLASEEAGYITGANISVNGGMHMM"
     gene            complement(10555..11730)
                     /locus_tag="VIG70_06940"
     CDS             complement(10555..11730)
                     /locus_tag="VIG70_06940"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY8250327.1"
                     /translation="MEEIYIVGAARTPIGKFGGSLAKTPAADLGALIIRKVLERAGVA
                     PDQVSEVIMGQVLAAGVGQNPARQAAIKADLPHMIPAMTINKVCGSGLKAAMLGAQSI
                     AHGDADIVVAGGQESMSLSPHALMGSRDGFRMGDAKMVDTMIVDGLWDVYNQYHMGVT
                     AENVAKEYAVTREEQDEFAVTSQNKTEVAQKAGRFKDEIVPVEIPSRKGPVMFADDEY
                     PRHGATLEAMKSLKPAFDKNGSVTAGNASGINDGAAAVVLASAKKAKELGLKPLARIK
                     AFASAGVDPKYMGMGPVPASKRTLSRAGWEPKDIDLMEINEAFAAQAIAVNRQMGWDT
                     KKVNVNGGAIALGHPIGASGCRILVTLLHEMQKRDARRGLASLCIGGGMGVALAVER"
     gene            complement(11741..13429)
                     /gene="phaC"
                     /locus_tag="VIG70_06945"
     CDS             complement(11741..13429)
                     /gene="phaC"
                     /locus_tag="VIG70_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004527151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEY8250328.1"
                     /translation="MQPGSPDALLAAIAQSSNEVTQGWMRVLASAPAAAGALPWSNSS
                     LQRSYFEKQARLWAALASGKRETLATPEPGDRRFAAKEWRENPYYDYLKQSYLLAAQY
                     LEELVQDAQLEPTAKERARFAARQWIDALCPANFPATNPEALRQALDTRGESLARGLA
                     NLLGDVQKQRISQTKESAFEVGRNLAVTPGDVVYENELIQLIQYRATTPSVARRPLVM
                     VPPCINKYYILDLQPENSFVAHAVAEGNTVFMVSWRNVEADQGRFGWDDYLEQGVFQA
                     LRVASEITKSEQVNALGFCVGGTLLGAALAVLSAKKQDPIASATLLAAMLDFSETGQI
                     GLFVDESSVAAREAAIGSGGIFPGRDLAFVFSSLRANDLIWPYVVNNYLLGGEPAAFD
                     LLYWNSDSTNLPGPMYCYYLRNTYLENRLRQPGALVNCGVPVDLGSVKLPVFVLATRE
                     DHIVPWRSAYRTLGLVGGGDKTFVLGASGHIAGVINPAAKKRRSHWVSDSYPADPQGW
                     LGVAVEKPGSWWPRWSQWLAKHHGGQRKAPAKQGSAKFKPIEPAPGRYVKHRIH"
     gene            complement(13505..14266)
                     /locus_tag="VIG70_06950"
     CDS             complement(13505..14266)
                     /locus_tag="VIG70_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY8250329.1"
                     /translation="MTLAWILAASIAGGTLSAGLAAASLALRATWVPMLVSFAIGALL
                     GAAFLEIIPHAFEQGEAHQVAFSILGGIFGFFVLEKLLLWRHCHTENCEVHDPHAAPH
                     DHGRSGALIIVGDTVHNFVDGVLIAAAFLQSIELGVVTAVAIIAHEIPQEVGDFLILL
                     HSGYTRLRALAMNLLSSVATVVGGVIGYFGLQLFEGLEATLLGIVAASMIYVAVADLI
                     PGLHRRPELRDTAFQALLIALGIGSIAMVRAVLPH"
     gene            complement(14280..14921)
                     /gene="pgeF"
                     /locus_tag="VIG70_06955"
     CDS             complement(14280..14921)
                     /gene="pgeF"
                     /locus_tag="VIG70_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY8250330.1"
                     /translation="MIKPDWPAPARVHAFATTRAFGDLKAQRTREKLRALLPAEPAWL
                     KQVHGKRVIDLDRDPPTEADAALTRRTGTVCVAQMADCLPVLFADEAGTTVASAHAGW
                     RGLAAGVLEATVEAMSVPRHKLLAWLGPAIGPRVYEVGDEVRAAFHGHEAAFTPTRPG
                     HWLLDLYAVARGKLTGLKSVSGGDFCTYSERERFFSFRRDRTAERMGAFIWLA"
     gene            complement(14908..15855)
                     /locus_tag="VIG70_06960"
     CDS             complement(14908..15855)
                     /locus_tag="VIG70_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY8250331.1"
                     /translation="MREKNYTDWDASFVAQMTESVPAALGGLRLDQALARLFPQYSRN
                     RLQAWLKSGHITIDGKRLDANYAVSGGEQIALRPPRVPDAAAPQAQRMPLKVVFEDAE
                     LIVIDKPAGLVVHPGAGQPDRTLLNALLAHAPALGAVPRAGIVHRLDKDTSGLLVVAK
                     TVSAQAELVKQLADRSMRRVYLAVVQGDPPASGRIDAPVGRDVRARTRMAVTHRGKPA
                     RTNYRVLERYGRAALVECRLETGRTHQIRVHFQHIRHPLVGDETYRRGTRHGVSFPRQ
                     ALHAAELALLHPRTRDVVSWKSPLPRDMKRLVDMLRDQA"
     gene            15854..16657
                     /locus_tag="VIG70_06965"
     CDS             15854..16657
                     /locus_tag="VIG70_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY8250332.1"
                     /translation="MRLSLLVVIAAFFVVGCASDIDDETAGWSAQRLYGEAKDAMSSS
                     DWPRAIKYLEKLEARYPYGRFAQQAQLEIAYAHWKDGERASGIAAVDRFIKMYPNHAN
                     ADYAWYLKGLINFNDQHGMLSHLATPDMSDRDPKSTRESFAAFKEVVTRFPDSRYAAD
                     AALRMRYLVNALASHEVHVARYYMKRGAYLAAANRAQYAIQHYPQAPALEEAVFVLVL
                     AYDRLGLDALRDAADRVMRSNFPESQYLKPGGERLAKGVPWWRLWDPDW"
     gene            16654..17598
                     /gene="denD"
                     /locus_tag="VIG70_06970"
     CDS             16654..17598
                     /gene="denD"
                     /locus_tag="VIG70_06970"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEY8250333.1"
                     /translation="MSKILVIGAAGMIGRKLAAQFARDELILQDVVPFDRESIVSDLS
                     APGEAEKLVASRPRLIVHLAAVVSGEAEADFEKGYRVNLDATRRLFEAVRNEGYKPRV
                     VFTSSIAVFGAPFPEAIGDEFLNAPLTSYGTQKAIGELLLSDYSRRGFIDGIGIRLPT
                     ICVRPGRPNKAASGFFSGIIREPLSGQEAVLPVPDGVRHWFASPRAAIDFLLHAAKMD
                     SGQLGARRNLSMPGISATVAEQIEALRKVAGDKVARLIRREPDPTIMRIVEGWPRNFD
                     AKRALALGFRADKSFEDIIRIHVEDELQKKPSPPPAHE"
     gene            complement(17534..19492)
                     /locus_tag="VIG70_06975"
     CDS             complement(17534..19492)
                     /locus_tag="VIG70_06975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY8250334.1"
                     /translation="MGQGGGAELKTALERAFAADGPLASAVGGFKLRPQQVEMAQAIH
                     DAMAHTGVLVAEAGTGTGKTFAYLVPALLTGGKVIVSTGTKTLQDQLFDRDLPAVRAA
                     LATGASVALLKGRANYVCLYRLRRAAEQATFSSREEISQLKRIERFAAVSATGDRADL
                     ADVPEDAPVWAHATSTRDNCLGQPCPDYGNCFVMRARRNALAADVVVINHHLFFADVV
                     LRDEGISELLPACNTVVFDEAHQLSETARLFFGESVSSAQLVDLARDARAELRVAGGA
                     SPEVERLANRLDKAARDLRLAFGDSTTRLAWTQALRVPGFAEILETTQQALKALRGPL
                     EAQAERSEGLESCARRTGVALATLVRLRETEPADEVRWVEVFAHAVRLHATPLSSAEL
                     FSRQMDDHPRAWIFTSATLAVGEDFGHFTRELGLPQAAARRWASPFDFARQALLYLPK
                     GLPGDPNEAAYTDAVIDAALPVLEASGGRAFLLFTTLRALRRAHERLRGSLRYPLLVQ
                     GTGSRSELLARFRALGNAVLLGSQSFWEGVDVRGEALSVVVIDKLPFAPPDDPVLAAR
                     IEAIRARGGNPFTELQLPQAVLSLKQGAGRLIRDEADRGVLMLCDPRLASKAYGRQIL
                     KSLPPMKLTRELAAVKAFFAIHPQHESG"
     gene            complement(19465..20202)
                     /locus_tag="VIG70_06980"
     CDS             complement(19465..20202)
                     /locus_tag="VIG70_06980"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEY8250335.1"
                     /translation="MFGLALAGFASVTFTQQALEIIPLRYRSVEQVLPALQPLVEPGG
                     TLSGSRGQLFLRASPGNVAEIRRALEAIDRPPKRLQISVRLDDAQDRERRGIEGSGTI
                     GSAGARVDISAQDSRRSAEERVDQRVQVLEGGRAMIFTGQSRPLRTPGGTVIQDIASG
                     FEVVPRLAGGGVSLDILQQRETPGAQPGSVQGQRLSTTITAPLGQWVEIGGTASSAAR
                     DDRGIASASSARGTGSRRVWVKVEELN"
     gene            20325..20738
                     /locus_tag="VIG70_06985"
     CDS             20325..20738
                     /locus_tag="VIG70_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY8250336.1"
                     /translation="MAWNDGATTDRDRPLIHALQALGVKYRYGGRSPETGFDCSGLIT
                     HVFERAWGMLLPPGTAALSKVGTAVKPKELEPGDLVFYNTRNRPYSHVGIYLGDGRFL
                     HAPRPGATVRVESVHTPYWRTRFNGARRLDPPSFS"
     gene            20810..21832
                     /locus_tag="VIG70_06990"
     CDS             20810..21832
                     /locus_tag="VIG70_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8250337.1"
                     /translation="MTVFKGIVLALAGLLAGSASAQDQVLNLYSSRHYQTDEALYTNF
                     TKATGIKINRIEGGEDPLIERIRNEGERSPADVLITVDAGRLWRAEQMGLFQPVKSAM
                     LESRIPANLREPSGLWYGFSTRARVIAYNKATVKAGEIRNYEDLADPKWKGRVCMRSS
                     TNIYNLSLLGAIIDHLGEAKAEAWAKGVRTNLAVEPKGGDTDQLKAVASGQCDVTISN
                     QYYYARLLRSSKPDEREVGEKIGIVFPNQASWGTHVNISGAGVLKNAPHRDAAIKFLE
                     YLASDEAQRYFADGNNEWPVVKSVKVDNPVLKMLGEFKQDPLNVAVLGKNQPSSQKVY
                     DRVAWK"
     gene            complement(21855..23432)
                     /locus_tag="VIG70_06995"
     CDS             complement(21855..23432)
                     /locus_tag="VIG70_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY8250338.1"
                     /translation="MAALLVLPVVLVVASFFAGASDLWGHLAATALPRYVGNTALLLA
                     AVAFGVVTIGVVAAWLVTAYRFPGREWLEWALLLPLAMPAYVMAYAYTDWLQFAGPVQ
                     SALRAATGWQAREYWFPEVRSLPGAAAMLSFALYPYVYLIARTAFQDLSRSAIEAGRL
                     AGLGALGAFLRVAVPLARPAIVAGATLALMETLADFGTVSYFGVEVFTTGIFKAWLSM
                     GDSVAAAQLSTCLLGFVVVLLALERANRGRAAYHGIAPRKARAHRLRGIGALGALVAC
                     AAPVVFGFALPALLLAVLAFHETMPLARLGSLVANSFGIAALTALIAVGIALVMVYAA
                     RLTRSPLVAGANRVAGLGYAVPGAVIAVGVLVPLGRLDNALAGWLEGAFGVRFGLLLT
                     GTIVALVYAYLVRLLAVALQTTDAGLAKITPSMEDAARSLGATPAAALARVHVPLLAP
                     SLATAALLVFVDVLKELPATFALRPFNFDTLAVEAYNLAKDERLAEAALPSLAIVAAG
                     LLPVIYVSRRYFQGVRG"
     gene            23535..24566
                     /locus_tag="VIG70_07000"
     CDS             23535..24566
                     /locus_tag="VIG70_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8250339.1"
                     /translation="MLLEVENLRHAYGDQEVVRDLSFSLARGAIGCLLGPSGCGKTTV
                     LRCIAGFEGIREGEIRLAGRVVSGAGTMVPPEKRRIGMVFQDYALFPHLSVAGNIAFG
                     LHALPPAERRARAAGMAELVGLSGALDKYPHEISGGQQQRVALARALAPRPELLLLDE
                     PFSNLDVDLRERLSLEVRDIIKASGATAVLVTHDQQEAFAMADEIGVLHDGRIQQWDS
                     PYNLYHRPANRFVADFVGQGVFLPAKALNGRQLQIELGVLHGDSQGIENLEVLLRPDD
                     VVHDDAAPTQAEVVHKAFRGAEILYTLRLASGRKVLALVPSHHNHALGEKIGIRLDVD
                     HVVAFRPET"
     gene            complement(24563..25294)
                     /locus_tag="VIG70_07005"
     CDS             complement(24563..25294)
                     /locus_tag="VIG70_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY8250340.1"
                     /translation="MIDNRNRAFHYAVLASLALHALLLFAFPDFIDTARRAASLPPQI
                     IARLIAPEPAPDPVVPVEPVRREAPPPPKAAKPARPEALPEAPPAPKPAPVLSTPQPA
                     PVPAPPVQAAAPPSPPVAALEPQPQPQSASPPQASPPPPAQAPESLSRDQYRLQLIDE
                     ARRHKRYPPLARENNWQGDVRVAVAIAANGRPSVTLKGSSGYEVLDRQALEMFTQAAR
                     AVPIPPALRGKEFALEVRAVYGLED"
     gene            complement(25284..27602)
                     /locus_tag="VIG70_07010"
     CDS             complement(25284..27602)
                     /locus_tag="VIG70_07010"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8250341.1"
                     /translation="MSAPALQIQFDPASARFGASRSQKRLEDDRLLLGRGVFSDDRDF
                     SGAAWLVLVRSPHAHARIKEIHLEGIRKAPSVIAAWSMADLRADGVKHIPVPPLFKRA
                     DGSPMAAPPRTPLAEGKVFYVGQPVVAIVAGTRQQAQDAAELVEIEYEDLPAVVDPRK
                     AIAPGAPLLWEAAGGNVAAEAAYGNREAVEKAFAEAAHVTRIELHNQRLNAMAMEPRC
                     AIGVHEAGRTMLYTQSQQPTSARDLLGAVFGAKPQDFRVINGDIGGGFGMKTGLTPED
                     ALVCYAARKLGRPVKWRAERSEEFVAAHMARDQHYSAALALDREGHILALRMEMLANI
                     GAVPVGSSAMIPLAMVAKVATSAYRVPAVDYRIKGVLTNTMATGAYRGAGRPEGNYLI
                     ERLLEKSARETHIDRIELRRRNLLTPDELPYKTHVGETYDSGEFERMLEGALRASDWE
                     GFSRRKKESSSRGKLRGRGVSVYLEWTGALPTETVDIELDAHGMVTVFSGTQAMGQGL
                     ETTYTQLVTEVFGIASDKVRIVQGDTDHANGVGSVGSRSAFVGGSAVVAAGRKAVVEC
                     KTLAAEALEAAAGDIELVDGRLRISGTDREISFAELAARQPAKLIRVSATQTPSTPSW
                     PNGAQVAEVEIDPETGEVALARLSSCDDIGRIINHAIVEGQIHGGMAQGAGQALWEQV
                     RYDPDSGQLASGSLMDYCVPRADQLPPLQARFDESVPCKTNLLGVKGCGELGTIGAVP
                     AVVHAVLDALDLPHLEMPLTSEKIWRALRRDR"
BASE COUNT         4739 a         9489 c         9343 g         4531 t
ORIGIN      
        1 ggaggtgaag ctgcgcggcg accggctgag cttcaagctc gccggccgca agggcgagtt
       61 cagcggccag gtgaaaggaa agagcatcga gggcgtggtc gagtccggcg gcagcaagtc
      121 gccctggagc gcgaccctcg gcgggtgaag atccgaatca gcgccgcggg cgcgagtctc
      181 gaagcggttc tcaaagacac cccgaccgca aaagcgctcg tcgccgccat gccgttcgag
      241 gcgcgcgccc agacctgggg agaagaggtc tacttcatca cgccggtcag cgccaggctc
      301 gagcccgatg ccaagcaggt cgtcgagccg ggcacggtgt gcttctggac cgagggcgat
      361 gcgctcgccc tgccctacgg ccgcacgccg atctccaccg acgcgcggcc caagctggca
      421 aatccctgca acctggtcgg aaccctggtc ggcgacccga aagcgctttc gcgcatcaag
      481 gcgggtgaaa aaattaccgt gagcaaggcg taatgcagcg caagcgggtg aattcctcga
      541 agctgcgctc ggtcggctac gacgagccga cgcgcacgct cgagatcgag atgtcgaacg
      601 gcgaggtgtt ccagtacggc ggcgtgtacc cggaggtcta tcgccggctg atggccgcgc
      661 ctaacccgac ttcgttctac gacgacaaga tcgccgacga atacaccgcg aagcgcgtct
      721 gacggcgtaa actagcggca agaattgcgg caagggaggc ggcaatgcgg cggggattga
      781 tcatggccat gctcggcctg gcgtgcgcgg ctgctcaggc ggcgacgccg cagcgcgagc
      841 tggcgcagcc cgcgccggtt tttgcgttcg acagcctgca cggcatcgtg agctggctca
      901 ggcagcacgg ccgcgaaggc tacatcggcg ccgacgtcgc agacgcgatg ggcatcccgc
      961 gcccggagga cgagccgctg gtcgcggcat ggcagcgcgg gttcaagaac gacgacgtgc
     1021 tgcgcatcgc gcaggttctg gacaaccccg cggccgacgg cgagcgcgat ttcatcctct
     1081 tcatggtgca gcggcccgat gaccaggtgt acttctacct ctcgacgccg ctcggcgggc
     1141 tgcagaaggc gttcctctcg atcccgagca agaacctggt gatgccgctc gagcgcgccg
     1201 aggcggaact gcgctttcgc gaagaggtcc tgtactggaa ggaccggagc gaagcacgtt
     1261 aatggaagcc gagcgcctca accagatcgc ctctcagctc accgacctgc aggcgcggtc
     1321 ggccgagctg cggaggtatc tttgacctcg aaggcaagca aaagaggctg actgaagtcg
     1381 agcgcgagct gagcgatccg caggtctgga gcgaccagca gcgcgcccag gagctcggca
     1441 aggaaaagaa ggcgctcgaa tccgtcgtcg gcacgctcgg ccgtgtcggc agcggcctgt
     1501 ccgacgccgc ggagcttttc ggcatggcgc gggcggagtc ggacgatgcc acgctcgccg
     1561 gggtcgcgtc cgacgtcgag aaaatcgaaa aggacgtcgc ggcgatggaa ttccgccgca
     1621 tgttctccaa cccgctcgat ccgaacaact gcttcatcga catccaggcg ggctcgggcg
     1681 gcaccgaggc gcaggactgg gcggcaatgc tcgagcgcat gtacctcaag ttctgcgacc
     1741 gccgcggctt cagcgccgag gtcctcgagc acaccccggg cgaggtggcg ggaatcaaga
     1801 gcgcctcgct caaggtgagc ggcgactacg cctacggcac gctgcgcacc gagaccggcg
     1861 tgcaccgcct ggtgcggaag agccctttcg atgccaacgc gcggcggcac acctcgttcg
     1921 ccagcgtgtt cgtctatccc gaggtcgacg agtcgatcga ggtcgaggtg aatcccgcgg
     1981 atctgcgcat cgacacctac cgcgcctcgg gcgccggcgg ccagcacgtc aaccgcaccg
     2041 acagcgcggt gcgcatcacg catctgccga cgaacaccgt ggtgcaatgc cagaacgacc
     2101 gctcgcagca tcgcaaccgc gccgaggcaa tggcgatgct gaaggcgaag ctctacgagc
     2161 tcgagatacg caagcgcctg gcggagaagc agaagctcga ggacgcgaag accgacatcg
     2221 gctggggcca ccagatccgc tcctacgtgc tcgaccagtc gcgcatcaag gacctgcgca
     2281 ccggcgtgga gaaaggcgcg acgcaggcag tgctcgacgg tgatctcgac gacttcatca
     2341 ccgccagcct caagcaagga gtctaggttg gaagaagaca acaagctggt cgacgaacgc
     2401 cgcgagaagc tgaaagcgct gcgcagcgcc gggcctgcgt acccgaacga tttccgccgc
     2461 gagcatctcg ccggcgatct acacgcgtcc cacggcaaca agatcaagga agcgctcgaa
     2521 aaggcggtca tcccggtgaa ggttgccggc cgcatgatgc tcaagcgcgt catgggcaag
     2581 gcaagcttcg ccaccctgca ggacagctcc ggccgaatcc agatttatgt ttcgaatgag
     2641 gtagaaaact acgaagcttt caagcactgg gacctcggcg acatcctcgg cgtcgagggc
     2701 ctgctcttca agacgatgaa gggcgagctc acggtcagcg caaggaagat ccgtctgctc
     2761 gccaaggccc tgcgcccgct gccggagaag ttccacggcc tcgccgacca cgagatgcgc
     2821 taccgccagc gctacgtcga cctgatcgtc aatccgcagg tgaaggatgt ctttttcaag
     2881 cggaccagga tcatccagac cctgcgcgag gtcctggccg ccgacggcta cgtcgaggtc
     2941 gagacgccga tgatgcagcc gatcccgggc ggcgccgcgg cgcggccttt caagacgcac
     3001 cacaacgcgc tcgacatgga gctctacctg cgcatcgcgc cggagctcta tctcaagcgg
     3061 ctggtggtcg gcggcctgga gcgcgtgttc gagatcaacc gcaatttccg caacgagggc
     3121 atgtcgacgc gccacaaccc cgagttcacg atgctcgagt ggtactgtgc ctaccaggac
     3181 tggcgctaca tgatggagca caccgagaag ctggtgcgcg ctgcggcggg cggcgcagtg
     3241 gtcacgtatc agggcaagca gatcgacttc ggcaagcctt tcgcgcgcat cacgatgccg
     3301 gacgcgatcc gcaaacaggg gatcaccggg gatctgcggg atcgcgcatt tctctcgaaa
     3361 tcgctgaccg cgatgaaaat cgagcatgaa aaacaccacg gctggggcgc gctgcaactg
     3421 atgctgttcg aggcggtagc ggagaagcac ttggtcgagc cgacgttcgt caccgacttt
     3481 ccagccgagg tatcgccgct ctcgcgccgg cgcgacggcg atcccgaggt cgcggaccgc
     3541 ttcgagctct tcatcgacgc gaaggagatc gcgaacgggt tctccgagct gaacgacccc
     3601 gaggatcagg cacaacggtt tctggaacaa gtgaagaaga aaaatgcggg ggatgacgag
     3661 gcgatgcact acgacgccga ctacatccgc gcgctcgagt acggcatgcc gccctgcgcc
     3721 gggggcggcc tgggcatcga ccggctggtg atgctcctga ccgacagtcc gagcatccgc
     3781 gacgtgatcc tgttcccgca gatgcggcct gaagtttgaa ccgaacggtt tagatacatc
     3841 cggtaacaaa cgctcaccgt cagtcgtcgg cgctccccgt agcctgggcg ttgttcgcag
     3901 cacgaaagga gaacaacgcc atgcaagcca cagccagccg cctgcacccg ctcctgaccc
     3961 tcgcggcgat ttccgtcacc gtgctttccg cggtgggcgt cgccagcctg accggcatga
     4021 ttccgtcctc gaagagccag gagaacacgc tgcagctccc aagcgaggtc ctgaagccga
     4081 tcgagccggc gatcagccat ccggtgacca agcctgcggc gaggaagatc gtcgctcgaa
     4141 aagccgcgcc gcagccggtc gagccgaccg ttctagtttc agaaactccc aaatcgcagc
     4201 cgctcgtcgg tcaactcgcc gtcgtggaat cggtgcgcga ggtgaaggag cccggcgacg
     4261 cgaagggcgt gggcgcggtg gccggcggcg tcgtcggcgg cgtgctcggc aacaagctcg
     4321 gcaaaggcaa aggcctggtc acgattctcg gcgcggccgg cggcgccttc gccgggcacc
     4381 agatcgagaa gcaggcgcgc gccgagaagc gctgggagat cgcgctgcgc ctcgatgacg
     4441 gctcgcaacg cacgctgtcg agcgacgcgg agccggcgtg gcgcacgggc gatcgcgtgc
     4501 gcctggtgga cgaccggctg gagcggatct agcccagaac ctgcttgaga agggggccca
     4561 tgcggtccac cgcggcgcgg ccctcagcga tcgcctcccc ggcgcggtgg aagtccatgg
     4621 tggcgaagtc gggcaggagc ggggtgatca ggacgtcggc gggctcgccc gcgaggcggc
     4681 tgcgcgcgac gcgcatctcc acgatgtcga gcgaggtcag gaagacgttg aagatcgaag
     4741 gaatcgacgg ctcgtcctgg ctgacgatcg acgggttgtc cggcttgccc ttgcgctgcg
     4801 ccttgccggg cacaaagcgg cccagccagc tcggctcctc gcgcggcgca acctcgcgct
     4861 ccttggtgcg gcggtagggg ccgagcttgc cccagctcag gtccactgcc accaccaggt
     4921 cggcgccgag cgcgcggcac atcgagaccg gcaccggatt gaccagcccg ccgtcgacca
     4981 gcactacgcc gtcgcggatc accggcgtca ggaggcccgg catggcgcag gaggcgcgca
     5041 ccgcgtcgag caccttgccg gtgcgcagcc agagctcgcg gcccgactgc agctcggtgg
     5101 cgaccgcgcc gtacgcgatc ggcagctccg agatatcgcg gtcctggagg atggtgcgga
     5161 agagcgtgaa gaggcgcgtg ccgcggatca ggccgccgcg ccaagtgagg tccatcagcc
     5221 gcaccaccgt agtccaggca aggccggtga cccattgctc gagccgctcg agctcgcccc
     5281 cggcgtaggc ggcgccgacc agcgcgccga tcgaagtgcc gcacaccagg tcgggcttga
     5341 tgccgcgctc ctcgagcgcg cggatcacgc cgatgtgcgc ccagccgcgc gagctgccgg
     5401 cgccgagcgc gagtccgagg cggagcttgc ggggcatgaa tcagggacag gctacgttga
     5461 tcggtgaaag cgcgattttg ccagccgaaa cgccacccgc ggggacgacc ataacagtcg
     5521 ggttttcaaa accttgccac ccccttggcg acggtcgttg cgcaggctgc gcgaggctat
     5581 gctagcccca tgcgccaatc gcgcatcgaa accgcctgtg cggagatccg ccgggcgctc
     5641 ctggagcgca ggcgggccat tgccgaagag atcgctcgtc atccacggcc gatcaccgcg
     5701 tgcgacgtgc atttcaaccg catgctcgag gagcgggctt cgatttcgca ggagatcgat
     5761 cgcttcgatg cgttcatctc gacgtcggag ttgttgacgc cggcggcgaa ggccagggtc
     5821 ctcgtctccg aggaaaacgt ggtctgtccc tgatttccgg ttctagagcc gctcgaagat
     5881 cgccgcgatg ccctgcccgc cgccgatgca catggtgacg agcgcgtagc gcttctgggt
     5941 gcgctcgagc tcgtagagcg ccttcaccgt aaggatgcag ccggtcgcgc cgatcggatg
     6001 gccgagggcg actgcgccgc cgttcggatt cacccgcgcc gggtcgaggc cgagatcctt
     6061 ggtgaccgcc atcgcctgca ccgcgaaggc ctcgttcgat tccaccacgt ccatgtcggc
     6121 gggcttgagg ccggcttttt cgaacacgcg cttcaccgcg gggatcggcc cgaggcccat
     6181 gacctgcggg tcgacgccgg cgtgcgcgta cgcgacgagg cgcgccatcg gcttcagatt
     6241 gttcttttcc gcaaaagaag aagaagtcag aacaacggca gctcccgcgt cgttgatgcc
     6301 ggaggcattg ccggcggtga ccgtgccgtc cttcttgaac acggtcttta gcttcgccat
     6361 gtcctctggc ttgatgtcct tacgcacgtg ctcgtcggac gtgaactgct cgacgccctt
     6421 ccgggtcttc agttcgatcg gcacgatttg cgacttgaaa tggccggcct cgatcgccct
     6481 ggcagctttc tgatgcgaag aaatggagaa ttcgtcctgc tgctgccggg tgaaaccgta
     6541 cttcgcggca atgttttcgg cggtgacgcc catgtggccg tgcccgaacg gatcgtgcag
     6601 cgcgccggtc atcgcatcga ccaccgtcgc gtcgcccatg cgctggcccc agcggccggc
     6661 gggcaggaag taggcggcac ggctcatgct ctcggcgccg ccggcgacgg cgacgtcgca
     6721 gtcgccgatc atcaggtgct gcgccgccga gacgatcgcc tgcaggcccg agccgcacag
     6781 ccggttgacg gtgaggcacg gcgtgccgac cggaatcccg gcctcgatcg ccgccacccg
     6841 cgacaggtac atgtccttcg cctcgccgtg gatcacgctt cccatgacga cgtggccgac
     6901 gcgcgccggc tcgatcttcg cgcgcgccac cgcttccttg atcgcgacgg cgcccagctt
     6961 ggttgcggca agctccttga gggcgccgcc gtagtcgccg atgcccgtgc gcacgcccga
     7021 aaccaccacc acctgattgc tcattgcagt ctcccgtaga gatcgtgctc gccggcacga
     7081 tcgatcgtca cgtccctgaa ttctcccgct ttgccgccct tgaagcgcac cacgccgtcg
     7141 atttccggcg cgtctgcgct cgagcggccg acgccgggct cgtcgaccag caccttgacc
     7201 gtccggccga ccttgcgctt cagcttggat gcgctgatgg cttcctgggt cttcatgaat
     7261 tgctctttcc ggtagcgctt gacctcgggg gaaagcgggt cgggcagcgc gttcgctgcc
     7321 gcgccctcga ccggcgaata ctcgaagcag ccgacgcgat cgagctgagc ttccttcaga
     7381 aaagagagca gctcagagaa atcatcctcg gtctcccctg gaaagccgac gatgaaggtc
     7441 gagcgcaggg tgatgtcggg gcacaccgcg cgccagccgc ggatgcgctc gagcactttc
     7501 tcggcgttcg ccggccgctt catcagcttg aggatgcgcg ggctcgcatg ctggaacggc
     7561 acgtcgaggt acggaaggat ctttccttcg gccatcatcg ggatgacctc gtcgacgtgc
     7621 ggatacgggt acacgtaatg cagcctcacc catgcgccga gctgtcccag gctttcgcag
     7681 agctgggtca tgcgcgtctt caccgggcgg ccctgccaga agccggttcg atacttcacg
     7741 tcgacgccgt acgcgctcgt gtcctgcgag atcacgagca gctccttcac gccgcccctc
     7801 accaggttct cggcttcctg caggacttcg ccgatcggcc gcgacacgag atcgcctcgc
     7861 atcgaaggga tgatgcagaa cgtgcagcga tgattgcagc cctcggcgat cttcagatac
     7921 gcatagtgcg gcggcgtgag ctttaccccg atcaagtccg tgtacggatc gtgcggcttg
     7981 ggaagatggg cgtgcacggc gctcatcacg gccggcgtgt cgtgcgggcc ggtgatggcc
     8041 agtaccgccg ggtgcttcgc tcttatgaag tcgttcttga cgcccaaaca gcccgtgacg
     8101 atcaccttgc cgttctcggc gagcgcctcg ccgatcgcgc cgagtgattc ctcgaccgcc
     8161 gcgtcgatga agccgcaggt gttcacgatc accaggtcgg cgtccgcgta cgtcttcgag
     8221 gtctcgtagc cttccgcgcg caactgcgtg aggatgcgct cggagtcgac gagcgccttg
     8281 ggacagccga gggaaacgaa gccgactgta ggcgcctttg tcctgctact gcttttcagg
     8341 atcgctcttg cccgtctggg agaagccggg gaaggacagg cccgagaaca tgttgcgcgc
     8401 ctggctctgc atctggtcct ggaactgcgc gaacatcttc tggctctgct ccatgtaggc
     8461 ctgcatcagg ctctgcatcg ccggcccctg caggttcatg aactgcgccc acagctcctg
     8521 cgaggcgcgg gagttgtcgc cgtacatcct ggtcgactgc tcctgcaggg tcttctggat
     8581 gtcgtgaaac gctcggatgt tcttctcgag atacgtgccc atcagcccct gcatggcgtg
     8641 gccgtagaat cggatcagct gcgacagcag ctcggaggag aacatcggca cgccgcccga
     8701 ctcctcctcg aggatgatct gcagcaggat ctggcgcgtc aggtcctcgc cgctcttcgc
     8761 gtcgaccacc tggaactctt cctgcttcag gaccatctgc ttgacgtcgg cgagcgtgat
     8821 gtagctcgac gtcttggtgt cgtacagccg ccggttgggg tacttcttga tcagccggag
     8881 ctggttctcc atcttcgaat ggttcttgga atggttcgct tcggtcagcg ccacagatgc
     8941 aatctactcc ttaatacatg tgctggccac cgttgatcga gatgttggcg ccggtgacga
     9001 acgcggcctc ctcggagcac agatagatga tcaggcccgc cacctcgtcg ggcttgccga
     9061 ggcgcccgat cgggatctgc ggcaggatct tggagtcgag gatctccttc gggatcgcgg
     9121 tgaccatctt ggtgccgatg tagccgggcg agatggtgtt gacggtgacg cccttgcgcg
     9181 ccacctcgag cgcgagcgcc ttggtgaagc cgtggatgcc cgctttcgcc gccgagtagt
     9241 tggtctggcc gaacgcgcct ttctggccgt tcaccgagga cacgttgatg acgcggcccc
     9301 agccgcgatc gaccatgccg tccatcacct gcttggtcat gctgaaacac gagtcgaggt
     9361 tggtgcgcat cacggcgtcc cagtccgcct tgctcatctt cttgaaggtc atgtcgcgcg
     9421 tgatgccggc gttgttgacc aggatgtcga ccgggcccac ctcggcctgg atggctgcga
     9481 tcttgcgcgc gcagtcgtcg tagtcgacca catccaccgg aaaggcggcg aacgaatggc
     9541 ccttgccctt catctcctcg agccacgact tgtacttcgt gttggacggc gaataggtga
     9601 ccaccacgcg gtagcccgcg cccgccatct tcagggaaat cgtctcgccg agaccgccca
     9661 tcccaccggt aaccacggcg acgcgctgct tggccattat ctcctccagc tcatgtccac
     9721 gagcgggaga aaagtctaca tcatgtgcat gccgccattg accgagatgt tggcgccggt
     9781 gatgtagccg gcttcctccg aggcgaggaa ggcgacgacg gcggcgatct cctcgggctt
     9841 ggcgagccgc cccatcggga tcgaggcgac gatctggtcg cggatttcct ggcggatggc
     9901 catcaccatg tcggtctcga cgtagcccgg cgacacggtg ttgaccgtga cccccttgcg
     9961 caccacttcc tgcgccagcg ccttggagaa cccggccatg cccgccttgg ccgccgagta
    10021 gttggtctgc ccgaactggc ccttgatcgc gttgaccgac gagatgttga tgatgcggcc
    10081 ccagccgcgc tcgctcatgc cgtcgatcac ctggcgcgtg acgttgaaca cgctgttcag
    10141 gttggtgttg atgaccgcca tccagtcctg ctcggtcatg cgcttgaaca ccgagtcgcg
    10201 cgtgatgccg gcgttgttga tcaggatgtc gatcgggccg accaccgagc gcacgttgta
    10261 gaacatctcg gcgcacgact cgaactccga cacgtcgccc tcggccgcgt gcacgcgaaa
    10321 gcccagcgcg cgcatggcgt cgagccactc ggtcttcttc tcgtagccgg gcaggcagcc
    10381 ggcgaccacg gtatgccccg cgcgcccgag gcggcggcag atcgcgctgc cgattccgcc
    10441 catgccgccg gagacgaagg cgatgcgcga aacgacgggc gaagactcgt cgcgccacgt
    10501 ctccggccgt tgtaccactt ctgcttggtt tcccatcttt gtcattttcc ctttctagcg
    10561 ttccacagcc agcgcgacgc ccatcccgcc gcctatgcac agcgacgcaa ggccgcgcct
    10621 tgcatcgcgc ttctgcatct cgtgaagcag cgtcaccaga atgcgacagc ccgaggcgcc
    10681 gatcggatga ccgagcgcga tcgcgccccc gttcacgttg actttcttcg tgtcccagcc
    10741 catctggcgg ttcacggcta tggcctgggc ggcgaaggcc tcgttgatct ccatcaggtc
    10801 gatgtccttg ggctcccagc cggcgcgtga cagcgtgcgc ttggaggccg gcaccggccc
    10861 cattcccatg tacttcggat cgacgccggc cgaggcgaat gccttgatgc gggccagcgg
    10921 cttgagcccg agctccttgg ccttcttcgc cgaggccaga accacggcgg cggcgccatc
    10981 gttgattccg gacgcgttgc cggcggttac gctgccgttc ttgtcgaaag cgggcttgag
    11041 gctcttcatc gcctcgagcg tagcgccgtg gcgtgggtac tcgtcgtcgg cgaacatcac
    11101 cggccctttg cgcgacggga tctcgaccgg cacgatctcg tccttgaatc tcccggcctt
    11161 ctgcgcgacc tcggtcttgt tctggctcgt caccgcgaac tcgtcctgct cctcgcgcgt
    11221 caccgcgtac tccttggcca cgttctcggc ggtgacgccc atgtggtact ggttgtagac
    11281 gtcccacagg ccgtcgacga tcatggtgtc gaccatcttc gcgtcgccca tgcggaagcc
    11341 gtcgcgcgat cccatcagcg cgtgcggcga caggctcatc gactcctggc cgccggccac
    11401 cacgatgtcc gcatcgccgt gcgcgatcga ctgggcgccg agcatcgccg ccttgagccc
    11461 ggagccgcag accttgttga tggtcatcgc cgggatcatg tggggcaggt cggccttgat
    11521 cgcggcctgg cgcgccgggt tctggccgac gccggcggcg agcacctggc ccatgatcac
    11581 ctcggatacc tggtcggggg cgacgcccgc gcgctccagc accttgcgga tgatgagcgc
    11641 gccgaggtcc gcggcgggcg tcttcgcgag gctgccgccg aacttgccga tcggggtgcg
    11701 ggctgcgccg acgatataga tttcttccat gggggctcct ctaatggatt cggtgcttca
    11761 catagcggcc gggcgcgggc tcgatcggct tgaacttcgc gctgccttgc ttcgcgggcg
    11821 ccttgcgctg gccgccgtga tgcttggcga gccattgcga ccagcgcggc caccagctcc
    11881 ccggtttttc aaccgcaacc cccagccagc cttgtggatc cgcggggtag gaatcggata
    11941 cccagtggct gcgccgcttt ttcgccgcag gattgatgac gccagcgatg tggccgctcg
    12001 cgccgagtac gaacgtcttg tcgccgccgc cgacgagccc gagcgtgcgg tatgccgagc
    12061 gccacggcac gatgtggtcc tcgcgcgtgg cgagcacgaa caccggcagc ttcacgcttc
    12121 ccaggtccac cgggacgccg caattcacca gcgcgccggg ttggcgcagc cggttctcga
    12181 gatacgtgtt gcgcaggtag tagcaataca tcggccccgg caggttggtg ctgtccgagt
    12241 tccagtagag caggtcgaac gccgccggct cgccgccgag aaggtagttg ttcaccacgt
    12301 agggccagat gaggtcgttc gcgcgcaggc ttgaaaacac gaacgcgagg tcgcgcccgg
    12361 ggaagatacc gcccgagccg atggccgcct cgcgcgccgc gacgctcgat tcgtccacga
    12421 acaggccgat ctgcccggtc tccgagaaat cgagcatcgc cgccagcaag gtcgcactcg
    12481 caatcggatc ctgcttcttt gccgagagaa ccgccaaagc agcgcccagt agagtcccgc
    12541 cgacgcaaaa gccgagcgcg ttgacctgct cgctcttcgt aatctcgctc gcgacgcgca
    12601 gcgcctgaaa cacgccctgc tcgaggtagt cgtcccagcc gaacctgccc tgatcggcct
    12661 cgacgttgcg ccacgaaacc atgaacacgg tattgccctc ggctacggca tgggcgacga
    12721 aggagttctc cggctgcagg tcgaggatgt agtacttgtt gatgcacggc ggcaccatca
    12781 ccagcgggcg ccgagcgaca ctgggggtcg tcgccctgta ctggatcagc tggatcagct
    12841 cgttctcgta caccacgtcg ccgggcgtga ccgcgaggtt gcggccgacc tcgaacgccg
    12901 attccttggt ctggctgatg cgctgcttct gcacgtcgcc gagaagattg gcgagccccc
    12961 gcgccaggct ctcgccgcgc gtgtcgagcg cctggcgcag cgcttcggga ttggtggcgg
    13021 gaaagttcgc cgggcacaac gcatcgatcc actggcgcgc agcgaagcgc gcgcgttctt
    13081 ttgccgtggg ctccaactgc gcgtcttgca ccaattcttc caggtattgc gccgcgagca
    13141 ggtacgactg cttcaggtag tcgtagtacg ggttctcgcg ccactctttg gcagcgaagc
    13201 gccggtcgcc gggctcgggc gtggcgagcg tctcgcgctt gcccgaggcg agggcggccc
    13261 aaaggcgcgc ctgcttctcg aaatagctgc gttgcagcga agaattgctc cacggaaggg
    13321 cgccggcagc agccggggca ctcgccagga ctcgcatcca gccctgggta acctcgttgc
    13381 tcgactgcgc gatcgccgca aggagggcgt ctggggagcc gggttgcatc gccgggcgat
    13441 tttgcgacgc acaacgagcc ccgtcaaatg attcttaaac cactgacgca atagtacttt
    13501 ttgcttagtg cggcagcacc gcccggacca tggcgatcga gccgattccg agggcgatca
    13561 ggagggcctg gaaagccgtg tcgcgcaact ccggccggcg gtgcaaaccg gggatcaggt
    13621 cggcgaccgc gacgtagatc atgctcgcgg ccacgatgcc gaggagtgtc gcctcgagcc
    13681 cttcgaagag ctgcaggccg aaatagccga tcaccccgcc gaccaccgtc gctaccgacg
    13741 acagcaggtt catcgcgagc gcgcgcagcc gtgtataccc cgagtgcagc aggatcagga
    13801 agtcgccgac ctcctgcggg atctcgtgcg cgatgatcgc caccgcggtg accacgccga
    13861 gctcgatgct ctgcaggaag gcagccgcga tcagcacgcc gtcgacgaag ttgtgcacgg
    13921 tatcgccgac gatgatcagc gcgccgctgc gcccgtgatc gtgcggcgcg gcatgcgggt
    13981 cgtggacttc gcagttctcg gtgtggcaat ggcgccagag caggagcttc tccagcacga
    14041 aaaagccgaa gatgccgccg aggatcgaga acgcgacctg gtgcgcctcg ccctgctcga
    14101 aggcgtgggg aatgatctcg aggaaggccg cgccaaggag cgcgccgatg gcgaacgaca
    14161 ccagcatcgg cacccaggtc gcgcgcagcg ctagcgacgc cgcggctaag ccggcgctga
    14221 gcgtgccgcc ggcgatgctt gcggcgagga tccaggcgag cgtcatgggg ggctggattc
    14281 tacgccagcc atatgaatgc tcccattcgc tcggcggtgc ggtcgcgccg aaaggagaaa
    14341 aagcgctcgc gttctgagta ggtgcaaaag tcgccgcccg agacgctttt caggccggtg
    14401 agcttgcccc gcgccactgc gtacaagtcg agcagccagt gcccggggcg agttggcgtg
    14461 aacgcagcct cgtggccgtg gaaagccgcg cgcacctcgt cgcccacttc gtaaacccgc
    14521 gggccgatgg ccggtccaag ccacgcgagt agcttgtgcc ggggcacact catcgcttcc
    14581 accgtggcct cgagcacgcc ggcggccagc ccgcgccacc cggcgtgcgc cgacgccacg
    14641 gtcgtgccgg cctcgtcggc gaacagcacc ggcaggcaat cggccatctg cgccacgcac
    14701 acggttccgg ttcttcgcgt cagggccgcg tccgcctccg tcggcgggtc gcggtccaaa
    14761 tcgatgacgc gcttgccgtg aacctgcttc agccacgcgg gctcggccgg aagcagcgcg
    14821 cgcagcttct ccctggtgcg ttgagctttg agatcaccga aggcgcgggt ggtggcgaat
    14881 gcatgcacgc gcgccggcgc cggccagtca ggcttgatca cgaagcatgt ccacgaggcg
    14941 cttcatgtcc cgcggcaagg gcgatttcca cgagaccacg tcgcgggtgc ggggatggag
    15001 cagcgccagc tccgcggcat gcagcgcctg gcgcggaaag ctcacgccgt gccgtgtgcc
    15061 gcgccggtag gtttcgtcgc cgaccagcgg gtggcggatg tgctggaagt gcacgcggat
    15121 ctggtgcgtg cggccggtct cgagccggca ctcgaccagc gcggcgcgcc catatcgctc
    15181 gagcacgcgg taattcgtgc gcgcgggctt gccgcggtgg gtgaccgcca tgcgcgtgcg
    15241 cgcgcgcacg tcgcggccga cgggcgcgtc gatcctgccg ctcgccggcg gatcgccctg
    15301 caccaccgcg aggtagacgc ggcgcatgct gcggtccgcg agctgcttca cgagctcggc
    15361 ctgagccgac acggtcttcg ccaccacgag gagcccgctc gtgtccttgt cgagccggtg
    15421 cacgatgccg gcgcgcggca ccgcgccgag cgcgggcgcg tgcgcgagca gcgcattgag
    15481 cagcgtgcgg tccggctggc cggcgccggg gtgcaccacc agcccggcgg gcttgtcgat
    15541 cacgatgagc tcggcatcct cgaacaccac tttcagcggc atgcgctgcg cctgcggcgc
    15601 ggcggcgtcc gggacgcgcg gcggacgcag cgcgatctgc tcgcctcccg aaaccgcgta
    15661 gttcgcgtcg aggcgcttgc cgtcgatcgt gatgtggccg gacttgagcc acgcctgcag
    15721 ccggttcctc gaatactgcg gaaagagccg cgccagcgcc tggtcgaggc gcagcccgcc
    15781 gagggccgcg ggcacgcttt cggtcatctg cgccacgaag ctcgcatccc agtcggtata
    15841 atttttctcc cgcatgcgcc tcagtttact ggttgtcatc gctgcattct tcgtcgtcgg
    15901 gtgcgcgagc gacatcgacg acgaaaccgc cggctggtcg gcgcagcgcc tctacggcga
    15961 ggcgaaggac gcgatgtcct ccagtgactg gccgcgcgcg atcaagtacc tcgagaagct
    16021 cgaagcgcgt tacccctacg ggcgcttcgc gcagcaggcg cagctcgaga tcgcctacgc
    16081 gcactggaag gacggcgagc gggcgtcggg aatcgcggcg gtggaccgct tcatcaagat
    16141 gtatccgaat cacgccaacg ccgactatgc ctggtacctg aaggggctga tcaacttcaa
    16201 cgaccagcac ggcatgctgt cgcacctcgc gacgcccgac atgagcgatc gcgacccgaa
    16261 gtcgacgcgc gagtcgttcg ccgccttcaa ggaagtggtg acgcgcttcc ccgactcgag
    16321 atacgccgcg gacgcggcgc tgcgcatgcg ctacctcgtc aacgccctcg cctcgcacga
    16381 ggtgcacgtg gcgcgctact acatgaagcg cggcgcctac ctcgcggccg cgaaccgcgc
    16441 gcagtacgcc atccagcact acccgcaggc cccggcgctc gaggaagcgg tcttcgtgct
    16501 ggtgctcgcc tacgaccggc tcggcctgga cgcgctgcgc gacgcggccg accgcgtgat
    16561 gcgcagcaac ttccccgaga gccagtatct caagcccggc ggcgagcgcc tcgcaaaggg
    16621 cgtgccgtgg tggcggctgt gggatcccga ctggtgagca agatcctggt gatcggcgcg
    16681 gccgggatga tcggccgcaa gctcgcggcg cagttcgccc gggacgagtt gattctgcag
    16741 gacgtcgtgc ccttcgatcg cgagagcatc gtttccgacc tctcggcgcc gggcgaagcg
    16801 gaaaagctcg tcgcttcgcg tccccggctc atcgtccacc tcgccgccgt cgtctcgggc
    16861 gaggccgagg cggatttcga gaaaggctac cgcgtcaacc tggatgcgac gcggcgcctc
    16921 ttcgaagcgg tccggaacga ggggtacaaa ccgcgcgtgg tcttcacttc atccatcgcg
    16981 gtgttcggcg cgcctttccc cgaggcgatc ggcgacgagt tcctcaatgc ccctctcacc
    17041 agctacggca cgcagaaggc gatcggcgag ctgctgctct cggactacag ccggcgggga
    17101 ttcatcgacg gcatcggcat ccgcctgccg acgatctgcg tgcggccggg caggccgaac
    17161 aaggcggctt cgggcttctt ctcgggcatc atccgcgagc cgctctccgg ccaggaagcg
    17221 gtgctgccgg tgcccgacgg cgtgcgccac tggttcgcct ctccgcgcgc cgccatcgac
    17281 ttcctgctgc atgccgccaa gatggacagc gggcaactcg gcgcgcgccg caacctcagc
    17341 atgccgggga tcagcgcgac ggtggccgag cagatcgagg cattgcgaaa agtcgcggga
    17401 gacaaggtcg cgcgcctgat ccggcgcgag cccgacccca cgatcatgcg catcgtcgag
    17461 ggctggccgc gcaatttcga tgcgaaacgc gcgctcgccc tgggattccg cgccgacaag
    17521 agcttcgagg acatcatccg gattcatgtt gaggatgaat tgcaaaaaaa gccttcaccg
    17581 ccgccagctc acgagtgagc ttcatcggcg gcaggctctt caggatctgc cgcccgtagg
    17641 cctttgacgc cagccgcggg tcgcagagca tcagcacgcc gcggtcggcc tcgtcgcgga
    17701 tcagcctgcc ggcgccctgc ttgaggctca gcaccgcctg cggaagctgc agctcggtga
    17761 acgggttgcc gccgcgggcg cggatcgcct cgatgcgcgc cgcgagcacc ggatcgtcgg
    17821 gcggcgcgaa cggcagcttg tcgatcacga ccaccgacag cgcttcgccg cgcacgtcaa
    17881 ccccttccca gaaggactgg ctgccgagca gcacggcgtt gccgagcgcg cgaaaccgcg
    17941 cgagcagctc gctgcgcgag ccggtgccct gcacgagaag cggatagcgc agcgacccgc
    18001 gcaaccgctc gtgcgcgcgg cgcagcgcgc gcagcgtggt gaagagaagg aacgcgcgtc
    18061 cgccgctcgc ctcgagcacc gggagcgcgg catcgatcac cgcgtcggtg tacgccgcct
    18121 cgttcggatc ccccggcagc cctttcggca ggtacagcaa tgcctggcgc gcgaagtcga
    18181 acgggctcgc ccagcggcgc gccgccgcct gcggcaggcc gagctcgcgc gtgaaatgac
    18241 cgaagtcctc gcccacggcg agggtcgccg aagtgaagat ccaggcgcgc gggtggtcgt
    18301 ccatctgccg gctgaagagc tccgccgagg aaagcggcgt cgcgtgcagc cgcacggcgt
    18361 gggcgaacac ttcgacccag cgcacctcgt cggcgggctc ggtttcgcgc aggcgcacca
    18421 gtgtggcgag cgccaccccg gttcggcgcg cgcaggactc gaggccttcc gagcgctcgg
    18481 cctgcgcctc gagcggtccg cgcagggcct tcagagcctg ttgtgtggtt tcaagaattt
    18541 ccgcaaagcc gggcactcga agggcctgcg tccaggcgag gcgtgtcgtc gagtcgccga
    18601 acgcgaggcg caggtcgcgc gccgccttgt cgagacggtt ggcgaggcgc tcgacctcgg
    18661 gcgaggcgcc gccggcgacg cgtagctcgg cgcgcgcgtc gcgcgcgaga tcgaccagct
    18721 gcgcgctcga cacgctctcg ccgaagaaca ggcgcgcggt ctcggagagc tggtgcgcct
    18781 cgtcgaacac gaccgtattg cacgccggca gcagctcgga gatgccttcg tcgcgcagca
    18841 ccacgtcggc gaaaaagaga tggtggttga tcaccaccac gtcggcggcg agcgcgttgc
    18901 gccgcgcgcg catcacgaag cagttgccgt agtccgggca gggctgcccg aggcagttgt
    18961 cgcgcgtcga cgtggcgtgc gcccacaccg gcgcgtcttc cggcacatcg gcgaggtcgg
    19021 cgcgatcgcc ggtcgccgac acggcggcga agcgttcaat ccgtttcaac tgggagattt
    19081 cctcccggga gctgaacgtt gcctgctccg cggcgcggcg caatcgataa aggcaaacgt
    19141 agttggcccg gcccttgagc agggctacgc tcgcgccagt ggcgagtgca gcgcgcaccg
    19201 ccggcaggtc gcggtcgaat agctgatcct ggagcgtctt ggtcccggtg gagacgatca
    19261 ccttgccgcc ggtgagcagc gcgggcacca gatacgcgaa ggtcttgccg gtgcccgtgc
    19321 cggcctcggc cacgagcacc ccggtgtgcg ccatcgcgtc gtggatcgcc tgagccatct
    19381 cgacctgctg cgggcgcagc ttgaatccac cgacagccga ggcgagcgga ccgtcggcgg
    19441 caaaggcacg ctcgagcgcg gtcttcagtt cagctcctcc accttgaccc atacccggcg
    19501 cgagccggtg ccgcgcgcgc tgctcgccga ggcgatgcca cggtcgtcgc gcgcagcgct
    19561 cgaagcggtg ccgccgatct cgacccattg cccgagcggc gcggtaatcg tcgtggatag
    19621 ccgttgcccc tgtacgctgc ccggctgggc tcccggtgtt tcgcgctgct ggaggatgtc
    19681 gagcgacacg ccgccgcctg cgagcctggg cacgacttca aacccactcg cgatgtcctg
    19741 gatcacggtt cctcccggcg tgcgcagggg ccgggactgg ccggtaaaga tcatcgcgcg
    19801 cccgccctcc agcacttgca cacgctggtc gacgcgctcc tccgcgctcc ttcgcgagtc
    19861 ctgcgcgctg atgtccaccc gtgcgcctgc gctgccgatc gtgccgctcc cttcgatgcc
    19921 gcggcgctcg cgatcctgcg catcgtccag gcgcaccgag atctgcaggc gcttgggcgg
    19981 ccgatcgatc gcctccaggg cgcgcctgat ctcggcaacg ttgccagggc tcgcccgcag
    20041 gaagagctgg ccgcggctgc cgctcagggt gccgccgggc tcgaccagcg gctgcagcgc
    20101 gggcagcacc tgctcgacgc tgcggtagcg gagcggaatg atttcaaggg cttgctgggt
    20161 aaatgtgacg ctggcgaagc ccgccaaggc caacccgaac accaactgga ctagatgcga
    20221 gcgattcatc ccttccccat gaacgcgcca tccttcgcct cgtgcacttg cccattatca
    20281 gatacgcgct tctcctgctc gcatggcttt tcagcttgcc ggccttggcc tggaacgacg
    20341 gcgcgaccac ggatcgcgat cggcccctga tccacgcctt gcaggcgctg ggcgtgaagt
    20401 accgctacgg cggccgctct cccgagaccg gtttcgactg cagcggcctc attactcacg
    20461 tattcgagcg cgcatgggga atgctgctgc cccctggcac cgcggcgttg agcaaggtgg
    20521 gaacggccgt caaaccgaag gaactcgagc caggcgatct ggtgttctac aacacgcgta
    20581 accggccgta ctcgcatgtc ggcatctacc tcggggacgg gcgctttctc catgcgccac
    20641 gccctggagc aaccgtacgc gtagaaagtg tccacactcc ctactggcgt acgcgcttca
    20701 acggggcgag gcgcctcgat ccgccctctt ttagctgata atagttctcg ttgacagggt
    20761 tggatgagag tccctatcat ttggggctac ttctcaactc gaggtcgaca tgactgtttt
    20821 taaagggatt gtccttgcgc tcgccgggct gctggcaggc tccgcgtcag cgcaggacca
    20881 ggtactcaac ctctactcgt cgcgccacta ccagaccgac gaggcgctgt acacgaactt
    20941 caccaaggcc acgggcatca agatcaaccg catcgagggc ggcgaagacc cgctcatcga
    21001 gcgcatccgc aacgaaggcg agcgcagccc cgccgacgtc ctgatcaccg tggacgccgg
    21061 gcggctttgg cgcgccgagc agatgggcct gttccagccg gtgaagtcgg ccatgctgga
    21121 atcgcgcatc ccggcgaatc tgcgcgagcc aagcgggctt tggtacggct tctcgacacg
    21181 cgcgcgcgtc atcgcctaca acaaggcgac ggtgaaggcg ggcgagattc gcaactacga
    21241 ggacctcgcg gatcccaagt ggaagggacg tgtgtgcatg cgctcctcca ccaacatcta
    21301 caacctctcg ctgctgggcg cgatcatcga tcacctcggc gaagccaagg ccgaagcctg
    21361 ggcgaagggc gtacgcacca acctggcggt ggagccgaag ggcggcgaca ccgaccagct
    21421 gaaggcggta gcctccggcc agtgcgatgt cacgatctcc aaccagtact actacgcccg
    21481 cctgctgcgt tccagcaagc ccgacgagcg cgaggtcgga gagaagatcg gcatcgtgtt
    21541 cccgaaccag gcgagctggg gaacccacgt caacatctcg ggcgcggggg tgctgaagaa
    21601 cgcgccgcac cgcgacgccg cgatcaagtt cctcgagtac ctggcgagcg acgaggcgca
    21661 gcgctacttc gccgacggca acaacgagtg gccggtggtc aagagcgtca aggtcgacaa
    21721 cccggtgctg aagatgctcg gcgagttcaa gcaggacccg ctgaacgtcg cggtgctcgg
    21781 caagaaccag ccgagctcgc agaaggtcta cgaccgcgtc gcctggaagt aaggaagcgg
    21841 gggtgagaaa caccctaccc ccgcaccccc tgaaaatagc gcctcgagac gtaaatcacc
    21901 gggagtaatc ccgccgcaac gatcgccagc gaaggaaggg cggcttccgc cagccgctcg
    21961 tccttcgcca ggttgtaggc ctcgactgcc agcgtatcga agttgaacgg ccgcagcgcg
    22021 aaagtcgccg gcagctcctt caacacgtcc acgaatacca gcagcgccgc cgtagccagg
    22081 ctcggcgcga gcagcggcac gtgtacgcgc gcgagcgccg ctgccggggt agcgccgagc
    22141 gagcgcgccg cgtcttccat gctcggcgtg atcttggcga gccctgcgtc ggtggtctgc
    22201 agcgcgaccg ccagcaacct caccaggtag gcgtacacca gcgcgacgat ggtccccgtg
    22261 agcagtagtc cgaaccggac gccgaaggcc ccctcgagcc agccggcgag cgcgttgtcg
    22321 agccggccta gcggcaccag cacgccgacc gcgatcaccg cccccggcac ggcatagccc
    22381 agccctgcca cgcgattggc gccggcgacg agcgggctgc gcgtaaggcg cgcggcatag
    22441 accatgacca gcgcgatgcc gacggcgatc agcgcagtga gcgcggcgat gccgaagctg
    22501 ttcgcgacca gggagccgag gcgagccagg ggcatcgttt catgaaaagc aagaacggca
    22561 agcagcagcg ccggcagcgc gaaaccgaag accaccggtg cggcgcacgc gacgagcgcg
    22621 ccgagcgcgc cgatgccgcg caggcggtgc gcacgcgctt tccgcggcgc gatcccgtgg
    22681 taggcggccc ggccgcgatt cgcgcgctcg agcgctagca gcaccacgac gaagccgagc
    22741 aggcaggtgg agagctgcgc cgcggcaacg ctgtcgccca tcgacagcca ggccttgaag
    22801 atcccggtgg taaacacctc gacgccgaaa taggacacgg tgccgaaatc ggcgagcgtc
    22861 tccatcagcg cgagcgtcgc cccggcaacg atcgccggcc gcgcgagcgg caccgccacg
    22921 cgcaggaacg cgccgagcgc gccgagaccc gcgagccgcc cggcttcgat cgcgctgcgc
    22981 gagagatcct ggaacgcggt gcgtgcgatc aggtagacgt aggggtagag cgcgaacgaa
    23041 agcatcgccg cggcgcccgg aagcgagcgc acctccggaa accagtactc gcgcgcctgc
    23101 cagccggtcg ccgcgcgcag cgccgactgc accgggccgg cgaactgcag ccagtcggta
    23161 tacgcatagg ccatcacgta ggccggcatc gcaagcggta gaagcaaggc ccactcgagc
    23221 cactcgcgtc ccgggaagcg gtacgccgtg accagccacg cggccaccac gccgatcgtg
    23281 acgacgccga aagccaccgc cgcgagcagc aacgcggtat tcccgacgta gcgcggcaac
    23341 gcggtcgccg caagatggcc ccacaggtcg ctcgcgccgg cgaaaaagct cgccaccacc
    23401 aagacgaccg ggagcacgag cagcgcggcc accagcagcg aggttcctcc cagcagtcct
    23461 aaggtcttta aattggtctt taaattcaaa ggcttcgttt ttatcggaaa tgagaattat
    23521 tactataatc gagcatgctt ctcgaagtcg aaaacctgcg ccatgcctat ggcgaccagg
    23581 aggtcgtgcg ggacttgagc ttctcgctcg cgcgcggtgc catcggctgc ctgctcggtc
    23641 cgagcggctg cggcaagacc acggtgctgc gctgcatagc gggcttcgaa ggcatccgcg
    23701 aaggcgagat ccgtcttgcc ggccgcgtcg tctccggcgc cgggacgatg gtgccgcccg
    23761 agaaacggcg catcggcatg gtgttccagg actatgcgct attcccccat ctttccgttg
    23821 ccgggaacat cgccttcggg ctgcatgccc tcccgcctgc ggagcgccgc gcgcgcgccg
    23881 ccgggatggc ggagctggtg ggcctctcgg gcgcgctcga caagtacccg cacgagatct
    23941 ccggcgggca gcagcagcgc gtagccctag cccgcgcgct cgcgccgcgc ccggagctcc
    24001 tgctgctcga cgagccgttc tcgaacctcg acgtcgacct gcgcgagcgc ctgtcgctcg
    24061 aggttcgaga catcatcaag gcgagcggcg ccaccgcggt cctggtgacg cacgaccagc
    24121 aggaggcctt cgccatggcc gacgagatcg gcgtcctgca cgacggccgc atccagcagt
    24181 gggacagccc gtacaacctc taccaccggc cggccaaccg cttcgtcgcc gacttcgtcg
    24241 gccaaggcgt gttcctgccg gcgaaggcgc tcaacggccg gcagctccag atcgagctcg
    24301 gtgtcttgca cggcgatagc cagggaatcg agaacctgga ggtgctgctg cgccccgacg
    24361 acgtagtgca cgacgacgcc gcgccgaccc aggccgaagt ggtgcacaag gcgttccgcg
    24421 gcgccgagat tctctacacg ctgcgcctcg cgagcggccg aaaagtgctg gcgctggtgc
    24481 cgagccacca caaccacgcc ttgggcgaga agatcggcat ccggctcgac gtggaccacg
    24541 tcgtcgcttt tcggcccgaa acctagtcct ccagtccgta gacggcgcgc acttcaagcg
    24601 cgaactcctt gccgcgcagc gccggcggga tcggcacggc gcgcgccgcc tgggtgaaca
    24661 tttcgagcgc ctgccggtcg agcacctcgt accccgagct gcccttcagc gtcaccgacg
    24721 gccgcccgtt cgcggcaatg gccacggcca cccgcacgtc gccctgccag ttgttctcgc
    24781 gcgcgagcgg gggatagcgc ttgtggcggc gcgcctcgtc gatgagctgg agacggtact
    24841 ggtcgcgaga gagcgattcc ggcgcctgcg caggcggtgg cggggacgct tggggaggag
    24901 aagcggactg gggctgaggc tggggctcga gcgccgcgac gggcggagaa ggcggcgccg
    24961 cagcctggac cgggggcgcg ggtacggggg caggttgcgg cgtagaaagc accggcgctg
    25021 gcttcggcgc gggcggcgct tcgggcagtg cttcaggccg tgcaggtttc gcggcttttg
    25081 ggggcggcgg tgcttcccgc cgcaccggtt ccacgggaac taccgggtct ggagcgggct
    25141 cgggggcaat cagacgcgcg atgatctgtg gcggaaggct cgccgcgcgg cgcgcggtat
    25201 cgatgaaatc ggggaacgcg aacagcagga gcgcgtgcag cgccagcgat gcgaggaccg
    25261 cgtagtgaaa agcgcggttg cggttatcga tcacggcgca gcgcgcgcca gattttctcg
    25321 gaggtgagcg gcatctcgag gtgcggcagg tcgagcgcgt cgagcaccgc atgcaccacc
    25381 gctggaacgg cgccgatggt gccgagctcg ccgcagccct tgacgcccag gagattcgtc
    25441 ttgcagggga cgctttcatc gaagcgcgcc tgcagcggcg gcagctggtc ggcgcgcggc
    25501 acgcagtagt ccatgagcga gcccgaggcg agctggccgc tgtccgggtc gtagcgcacc
    25561 tgctcccaca gcgcctggcc ggcgccctgc gccatgccgc cgtggatctg cccctccacg
    25621 atcgcgtggt tgatgatgcg gccgatgtcg tcgcagctcg aaagccgcgc gagcgcgacc
    25681 tcgccggtct cggggtcgat ctccacctcg gccacctgcg ccccgttcgg ccaggatggc
    25741 gtggaagggg tttgcgtcgc ggagactctg attagcttgg cgggctggcg cgcagccaat
    25801 tcggcaaaag agatctcgcg atccgtgccc gagatgcgca gccggccgtc caccagctca
    25861 atgtcgccgg cagcggcctc cagggcttcc gcggccaacg tcttgcattc gacaacggcc
    25921 ttccgtcctg cggccaccac ggcggatcca ccgacaaatg cggagcgcga accgacgcta
    25981 cccacgccgt tcgcgtggtc ggtgtcgccc tggacgatcc tgactttgtc agaagcgatt
    26041 ccgaaaacct cggtcactag ctgcgtatac gtcgtctcga gaccttgtcc cattgcctgg
    26101 gtgcccgaaa acaccgtcac catcccgtgc gcgtcgagct cgatgtctac cgtctcggtg
    26161 ggcaacgcgc cggtccactc caggtagacc gaaacgcccc ggccgcgcag cttcccgcgg
    26221 ctcgacgatt ccttctttct ccgggaaaag ccttcccaat cgctcgcccg cagcgccccc
    26281 tcgagcatgc gctcgaactc gccgctgtca taggtctcgc cgacgtgcgt cttgtagggc
    26341 agctcgtcgg gcgtgagcag gttgcgccgg cgcagctcga tgcgatcgat gtgcgtctcg
    26401 cgcgccgact tctcgagcag gcgctcgatc aggtagttgc cctcgggccg cccggcgccg
    26461 cggtaagcgc cggtggccat ggtattcgtg agcacgccct tgatgcgata gtcgacagcg
    26521 ggaacccggt aggccgaggt cgccaccttc gccaccatcg cgagcgggat catcgccgac
    26581 gagccaaccg ggaccgcgcc gatgttggcg agcatctcca tgcgcagcgc aaggatgtgc
    26641 ccctcgcgat cgagcgccag cgccgccgaa tagtgctggt cgcgcgccat gtgagcggcg
    26701 acgaactcct cggagcgctc ggcgcgccac ttcaccgggc ggccgagctt gcgcgctgca
    26761 tagcacacca gcgcgtcctc cggcgtaagg ccggtcttca tgccgaagcc gccgccgatg
    26821 tcgccgttga tgacccggaa atcctgcggc ttcgcgccga acaccgcgcc gagcaggtcg
    26881 cgcgcgctgg tcggctgctg gctctgggta tacagcatgg tgcgcccggc ctcgtgaacg
    26941 ccgatcgcgc agcgcggctc catcgccatg gcgttcaggc gctggttgtg cagctcgatg
    27001 cgcgtgacgt gcgcggcctc ggcgaacgcc ttttcaactg cctctcggtt gccataagcc
    27061 gcttcggccg cgacgttccc cccggcggct tcccacaaga ggggcgcgcc cggcgcgatg
    27121 gcctttctcg gatccaccac cgccggcaga tcctcgtatt cgatctcaac gagctctgca
    27181 gcgtcctgcg cctgctggcg cgtcccggcg acgatcgcga ccaccggctg gccgacgtag
    27241 aacaccttgc cttcggcgag cggcgtgcgc ggcggcgcag ccatcggcga gccatcggca
    27301 cgcttgaaca gcggcggaac cgggatgtgc ttcacgccgt cggcgcgcag gtccgccatg
    27361 gaccacgcgg caatgaccga gggtgctttt cgaatccctt ccagatgaat ttccttgatt
    27421 cgtgcatggg catgcggaga gcgcacgagc accagccatg ccgcgccgga aaagtcgcgg
    27481 tcgtcgctga agacgcctcg gccgagcagc agccggtcgt cctccaagcg cttctgcgag
    27541 cgcgacgcac cgaagcgcgc cgacgccgga tcgaactgga tctgcagtgc tggtgcgctc
    27601 atctagggaa gtggcggaga gagagtctgc ccaaggccta gggtttacga ggatcttcag
    27661 gcatcgcgcg ccggtggcct agccggtggc ccagttcacc cggcgcgcag cgcagcgagc
    27721 tcgaggcgcg gcctgccggg cgtttcgggg cgagggaagc tcggcgtgca cggtgaggga
    27781 ttctagccgc cgccttgagt cctgctgcat ggtgtgcgag cgcgccgctt catggcgaag
    27841 cgccgcaggc ccggcagcga agaacggcaa ctcccgtcgc cgaggtgaaa ccctcacggc
    27901 tcacgccgcg tcagtcgagg tcatcgcggt cgatctaccc ccaagaacgg acgttctagt
    27961 gactggccgt gaacggtgat ttcgtgccaa tttgagacct gagcacttgc agcaagttgc
    28021 ggtactactc aatcacctca tcagcacgga caagcagcgt gggcggcaca gtaaggccaa
    28081 gcgccttcgc cgtcttgaga tt
//
LOCUS       DATBSZ010000108         6463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_766799, whole genome shotgun sequence.
ACCESSION   DATBSZ010000108 DATBSZ010000000
VERSION     DATBSZ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766799"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VIG70_07015"
     CDS             complement(<1..209)
                     /locus_tag="VIG70_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250342.1"
                     /translation="MAHTFLFEQGTWRVEGEHRDAAGAVSPVSGENSIRHEPGRWHFE
                     AALRLRGDPLRVTHNRYQIEPFSPGA"
     gene            complement(209..553)
                     /locus_tag="VIG70_07020"
     CDS             complement(209..553)
                     /locus_tag="VIG70_07020"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY8250343.1"
                     /translation="MIAVIFEFTAAEGRFADYKRLAEGLSEEVRNFDGFISIERFQSI
                     GDAARFVSLSFWRDEEAVRKWRNMQKHREAQAKGRGGIFSAYRLRICNVVRDYGMGER
                     GEAPKDSIAVHG"
     gene            600..1253
                     /locus_tag="VIG70_07025"
     CDS             600..1253
                     /locus_tag="VIG70_07025"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEY8250344.1"
                     /translation="MKPLPFKPHLGRIFLTGLLTLIPAVATVYLLVWLIGAVDRMFGK
                     PLRVLMPDETYLAGMGIVVAVAVVFGVGLLMHGVLFRKLFRRAEQAMLAVPLVRSIYS
                     AVKDLIGLFGRDGPAMQVVTLTFPGTSWRLLGFVTRAEFTDLPRGIGTAKEVAVYLPM
                     SYQVGGYTVIVPREAIHPVEMSREDAMKFILTAGLKTAGAPRTHSAAARAPGRTRPS"
     gene            complement(1183..2241)
                     /locus_tag="VIG70_07030"
     CDS             complement(1183..2241)
                     /locus_tag="VIG70_07030"
                     /EC_number="1.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HEY8250345.1"
                     /translation="MRSFVYSGLPARVVFGVGALSKLPEELDRLGAKHALVLSTPEQA
                     ESARSVKAALGARAVGLYDKAAMHVPVDIAEDARRTARELGADCCVTVGGGSTTGLGK
                     AIALTSTLPILAVPTTYAGSEMTTIYGITEGGAKKTGRDVRVLPKAVIYDPRLTLSMP
                     PGLSAASGMNAIAHAVEALYSHEGNPIVSLMAEEGIRALAVALPRILEESKNLDARSD
                     ALYGAWLCGIVLGSAGMALHHKLCHVLGGSFNLPHAETHSIVLPHAVRYNHAAAPDAM
                     ARIEKAIQASDAAAGIFDLEKRLGLPLRLADIGMKEADLERAARIATEAPYPNPRKVE
                     YAPVLELLRNAYEGRRPS"
     gene            complement(2250..3194)
                     /locus_tag="VIG70_07035"
     CDS             complement(2250..3194)
                     /locus_tag="VIG70_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250346.1"
                     /translation="MKKLLAAFAAIPFLALAQSYPARPVRVIVPFPPGGTTDLIARIV
                     QPRFQEYMGQSVLIENRSGAGGSVGAAEAAKAAPDGYTLLMVFDTHAVNHHIFKMAPD
                     PFKELEHISLMVTSPSAFVAVTSFAPNTMSEIVAYAKANPEKVTYSTPGSGSSNHLAA
                     LLFEQHAGIRLTHVAYKGGGPMIQALLGQQVNITFLSMPLILPHIKAGKVKAIATGGK
                     KRAPQLPDVPTLSETYPGFEQISWFGILGPAGLPQQVSTKVHREMTRTLRAPDVAQRL
                     TEQGFDIVASSPEEFFKYVQTESDKLGRLIRGNKIVAE"
     gene            3232..3630
                     /locus_tag="VIG70_07040"
     CDS             3232..3630
                     /locus_tag="VIG70_07040"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8250347.1"
                     /translation="MLKQQIRTAKLVDPGGHFSQAVAIPSKGVMVFVSGMTARRADGK
                     IAGIGDVEEQTRQVCENIKSALEAAGGSMDDVCRVDVYLRNIGDRERVNKVRREFFPS
                     PPPASTLVEVSKLASPEFLVEISAIAVISA"
     gene            complement(3608..4147)
                     /locus_tag="VIG70_07045"
     CDS             complement(3608..4147)
                     /locus_tag="VIG70_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8250348.1"
                     /translation="MTSVPEFSFSHMGIFVTDPGRMEDFYTRVLGFAVTDRGPLGSLT
                     LVFLSRDPQEHHQIVLASGRPASGGFNPINQMSFRMADFAGLREMHRRLQAEGVAELA
                     PVSHGNALSVYFKDPEGNRLELFVDTPWYVQQPLRVPMDMKLADAELWDWAEREARKQ
                     PGFKPVEEWRAALKLKLRQ"
     gene            4154..4999
                     /locus_tag="VIG70_07050"
     CDS             4154..4999
                     /locus_tag="VIG70_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8250349.1"
                     /translation="MKLATFKTAQGASYGAVTGKGIVDLRRYLGNQYADLKALIEGNA
                     LDQAKKHLSETPDYQPSDVTWLPVIPNPDKIVCVGLNYEEHRAETGRDKTEQPALFLR
                     VAESQVGHRQPIVRPRESTHLDFEAEIAIVIGQPGRRISQKDAFKHVAGYSCYNDGSV
                     RDWQRHTIQWTAGKNFARTGSFGPWMVTADEIPPNTVLTLSCRLNGERMQHATTDQMI
                     FKIPKLIEYISTFTTLLPGDVIVTGTPGGVGARRTPPLWMKPGDKVEIEIDKVGVLEN
                     TIGGD"
     gene            4999..5952
                     /locus_tag="VIG70_07055"
     CDS             4999..5952
                     /locus_tag="VIG70_07055"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250350.1"
                     /translation="MFRIFALVLACFAGPVFAQPYPSKPIRFIVSFPPGGSSDLIVRA
                     IAPRMGERLGQPVVVENRPGAGGMIGVEAVAKAPPDGYTIGLAAAGALSSNISLYPSM
                     PYHPEKDLAPVSMLAMIPFFLIAHPSQASSLKELIETARAKPGTLSYGYGGNGSTMHL
                     AGELFNRMANVKVQGVPYKGSGPVSADVLGGQVPLGVVDVPSAIANVKAGKIRALAVT
                     TKRRIAAAPDVPTFEEAGLPGYEAVGWFGTVAPAGTPANVVKKLNSEIRAALTVPDIR
                     ERALAAGAEPYTDTPEEFAEFIRAETKKWAQVIKDAGVKLQ"
     gene            5949..>6463
                     /locus_tag="VIG70_07060"
     CDS             5949..>6463
                     /locus_tag="VIG70_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3-carboxymuconate-6-semialdehyde
                     decarboxylase"
                     /protein_id="HEY8250351.1"
                     /translation="MTRSPVIDVHTHMLTNEWVSLLEQHGGPSYTLKPVAGGLKAIHL
                     DGAPFMTPVPNMFDWEARIANMNKARVDIAVTSLTCPNVFWGSAEASLKAARVANDDM
                     ARAQKTWPERIRWFASLPWQHEKLALEELARACSAGAVGVMVLANIGGKALTDESFKK
                     IWQEIDRRALPV"
BASE COUNT         1101 a         2169 c         2120 g         1073 t
ORIGIN      
        1 gcgccgggcg agaacggctc gatctggtag cggttgtgcg tgacccgcag cggatcgccg
       61 cgcagccgca gcgccgcctc gaaatgccag cggccgggct cgtgccggat cgagttctcg
      121 ccggagaccg gcgacacggc accggccgcg tcgcgatgct cgccctcgac gcgccaggtg
      181 ccctgctcga atagaaaagt gtgcgccact agccgtgcac ggcgatgctg tctttcggcg
      241 cctcgccgcg ctcccccatc ccatagtccc gcaccacgtt gcagatcctg aggcgatagg
      301 cgctgaagat gccgccgcgc cccttcgcct gcgcctcgcg gtgcttctgc atattgcgcc
      361 atttgcgcac cgcctcctcg tcacgccaga acgagagcga cacgaagcgc gccgcatcgc
      421 cgatgctctg gaagcgctcg atggagatga agccgtcgaa gttgcgcact tcctcgctca
      481 agccttcggc gagccgcttg tagtccgcga agcgcccttc ggcggcggtg aactcgaaga
      541 tgactgcaat catgctttcc ccctagtctg cgggaatcat ggcgcaaaac ggagggttca
      601 tgaagcccct gccttttaaa cctcacctgg gtcgcatttt cctcaccggg ctgctgacgc
      661 tgattccggc ggtcgcgacc gtctatctcc tggtctggct gatcggcgcg gtcgaccgca
      721 tgttcggcaa gccgctgcgc gtgctgatgc cggacgagac ctatctcgcc ggcatgggca
      781 tcgtggtcgc ggtcgccgtc gtgttcggcg tcggcctgct gatgcacggt gtgctgttcc
      841 gaaagctctt ccgcagagcg gagcaggcga tgctcgccgt gcccctggtg cggtccatct
      901 acagcgccgt caaggacctg atcgggctct tcggccggga cgggccggcg atgcaggtgg
      961 tcacgctgac cttccccggc acgagctgga ggctcctcgg cttcgtcacg cgcgccgaat
     1021 tcaccgacct gccgcgaggg atcggcacgg ccaaggaggt tgcggtctac ctgccgatga
     1081 gctaccaggt cggcggctac accgtgatcg tgccgcgcga ggcgatccat ccggtcgaga
     1141 tgtcccgcga ggacgccatg aagttcatcc tgacggcggg gctcaagacg gccggcgccc
     1201 ctcgtacgca ttccgcagca gctcgagcac cggggcgtac tcgaccttcc tagggttcgg
     1261 gtaaggcgcc tcggtcgcga tgcgcgcggc gcgctcgagg tcggcttcct tcatgccgat
     1321 gtcggcaagg cgcagcggca acccgagcct tttctcgaga tcgaagattc ccgccgccgc
     1381 atcgctcgct tgaatggctt tctcgatacg ggccatcgca tcgggcgcag ccgcgtggtt
     1441 gtagcgcacc gcgtgcggca ggacgatcga gtgggtctcg gcatgcggca gattgaaact
     1501 gccgccgagc acgtggcata gcttgtgatg cagggccatg ccggccgagc cgagaacgat
     1561 gccgcagagc catgcgccgt acagggcgtc gctccgggca tcgaggttct tggactcctc
     1621 cagaatgcga ggtaacgcaa cggccagggc gcgaatccct tcctcggcca tgagcgaaac
     1681 gatcgggttg ccttcgtgcg aatacagcgc ctcgaccgcg tgcgcgatgg cgttcatgcc
     1741 gctcgccgcc gacaggcccg gcggcatgct gagggtgagc ctggggtcgt agatcaccgc
     1801 cttgggcagc acccggacat cgcgcccggt cttcttcgcg ccgccctcgg tgatgccgta
     1861 gatcgtcgtc atctccgagc ccgcgtaggt ggtcggcacg gcgaggatcg ggagcgtcga
     1921 ggtcagcgcg atcgccttgc ccaggccggt cgtcgagccg ccgcccacgg taacgcagca
     1981 atcggcgccg agctcgcgcg ccgtgcggcg ggcatcctcg gcgatatcga ccggcacgtg
     2041 catcgccgcc ttgtcgtaca gcccgacagc gcgggcaccc aatgcggcct tcacggagcg
     2101 cgccgactcg gcctgctcgg gagtggaaag cacgagcgcg tgcttcgcgc ccagcctgtc
     2161 gagctcctcg ggcagcttgc tcagcgcgcc cacgccgaac accacccgcg cgggcaggcc
     2221 gctatagacg aagctccgca tcgtctcttc tattccgcca cgatcttgtt gccgcggatc
     2281 agtcttccga gcttgtcgga ctcggtctgc acgtacttga aaaattcctc cggcgagctc
     2341 gcgacgatgt cgaagccctg ctcggtcagg cgctgggcta cgtcgggcgc gcgcagcgtg
     2401 cgcgtcatct cccgatggac tttcgtggaa acctgttgcg gcaggccggc gggcccgagg
     2461 atgccgaacc aggagatctg ctcgaagccg ggataggtct cggacagcgt cggcacgtcg
     2521 ggcagctgcg gcgcgcgctt ctttccgccg gtcgcgatcg ccttcacctt gcccgccttg
     2581 atgtgcggca ggatcagcgg catggagagg aacgtgatgt tcacctgctg gccaagcagc
     2641 gcctggatca tcgggccgcc acccttgtag gcgacgtggg tcaggcggat gccggcgtgc
     2701 tgctcgaaga gcagcgcggc aaggtgattg gagctgcccg agcccggcgt cgagtaggtc
     2761 accttctccg ggttggcttt ggcgtaggcc acgatttcgc tcatcgtgtt cggcgcgaag
     2821 ctggtcaccg ccacgaacgc gctcggcgaa gtcaccatca ggctgatgtg ctcgagctcc
     2881 ttgaacgggt cgggcgccat cttgaagatg tggtggttca ccgcgtgggt gtcgaacacc
     2941 atgagcagcg tgtagccgtc gggcgcggcc ttcgccgctt ccgcggcgcc gaccgagccg
     3001 ccggcgccgg agcggttctc gatcagcacg gactggccca tgtactcctg gaacctcggc
     3061 tgcacgatgc gcgcgatcag gtcggtcgtt ccgccgggcg ggaaaggcac gatgacgcgc
     3121 accggcctgg ccggataaga ctgagcaagg gcaaggaagg gaatcgcggc aaatgcggcc
     3181 agcaattttt tcatggctgc ctcctcgcgg gccagtgtaa cctagcgccc catgctgaag
     3241 cagcagatcc gcaccgcgaa gctcgtcgat ccgggcggcc acttctcgca ggcggtcgcg
     3301 atcccgtcga agggcgtgat ggtgttcgtg tccggcatga ccgcgcgccg cgcggacggc
     3361 aagatcgccg gcatcggcga tgtcgaggag cagacgcgcc aggtctgcga gaacatcaag
     3421 agcgcgctcg aagccgcggg cggctcgatg gacgacgtgt gccgcgtcga cgtctacctg
     3481 cgcaacatcg gcgaccgcga gcgcgtcaac aaggtgcgcc gggagttctt cccctcgccg
     3541 ccgccggcgt cgaccctggt cgaggtgagc aagctcgcct cccccgagtt cctggtcgag
     3601 atcagcgcta ttgccgtaat ttcagcttaa gagcagcgcg ccattcctct accggcttga
     3661 accccggctg cttgcgcgcc tcgcgctcgg cccagtccca gagctcggcg tccgcgagct
     3721 tcatgtccat ggggacgcgc agaggctgct gcacgtacca cggcgtgtcg acgaagagct
     3781 cgaggcggtt gccttccggg tccttgaagt acaccgagag cgcgttgccg tgcgacaccg
     3841 gggcgagctc ggctacgccc tccgcctgca gcctgcggtg catctcgcgc agcccggcga
     3901 agtccgccat gcggaacgac atctggttga tcggattgaa cccgccggac gcgggccggc
     3961 cgctcgccag gacgatctgg tggtgctctt gcggatcgcg gctgagaaac acgagcgtca
     4021 gcgaaccgag cgggccgcga tcggtgaccg caaaaccgag cacgcgtgtg taaaaatcct
     4081 ccatccgccc ggggtcggtg acgaagatcc ccatgtggct gaacgaaaac tccggaaccg
     4141 aggtcatctc tctatgaagc tcgcgacgtt caagactgcg caaggcgcca gctacggcgc
     4201 cgtgaccggg aaaggcattg tcgatctgcg ccgctatctc ggcaaccagt atgcggatct
     4261 caaggccctg atcgaaggaa acgcgctcga ccaggcgaaa aagcatctct ccgagacccc
     4321 cgactaccag ccctccgacg tcacctggct gccggtgatc ccgaacccgg acaagatcgt
     4381 ctgcgtcggg ctcaactacg aggagcaccg cgccgagacc gggcgcgaca agaccgagca
     4441 gccggcgctg ttcctgcgcg tcgcggagtc gcaggtcggg caccgccagc cgatcgtgcg
     4501 gccgcgcgag tcgacccacc tcgacttcga ggccgagatc gcgatcgtca tcgggcagcc
     4561 cggaagacgg atttcgcaaa aggacgcttt caagcacgtc gccggctata gctgctacaa
     4621 cgacggctcg gtgcgcgact ggcagcgcca caccatccag tggaccgccg gcaagaactt
     4681 cgcgcgcacc ggctccttcg gcccgtggat ggtcaccgcc gacgagatcc cgccgaatac
     4741 cgtgctgacg ctgtcgtgcc gcctgaacgg cgagcgcatg cagcacgcca ccaccgacca
     4801 gatgatcttc aagatcccga agctcatcga gtacatctcg accttcacca cgctcctgcc
     4861 gggcgatgtc atcgtcaccg gcactccggg cggcgtggga gcgcggcgca cgccgccgct
     4921 ctggatgaag cccggcgaca aggtcgagat cgagatcgac aaggtcggcg tgctcgagaa
     4981 cacgatcggg ggggactagt gtttagaatt ttcgcgcttg tgctcgcgtg cttcgcgggc
     5041 ccggtgtttg cacagcccta tccttcaaaa cctatccgct tcatcgtgag cttcccgccc
     5101 ggcggcagct cggacctcat cgtgcgcgcg atcgcaccgc gcatgggcga gcggctcggc
     5161 cagccggtcg tggtcgagaa ccgccccggc gccggcggca tgatcggcgt cgaggcggtg
     5221 gcgaaggcgc cgcccgacgg ctacaccatc ggcctcgcgg cagccggcgc actctcgtcg
     5281 aacatcagcc tgtatccgag catgccgtac caccccgaga aggacctcgc gccggtctcg
     5341 atgctcgcga tgattccgtt cttcctcatc gcgcatcctt cgcaggcttc ttcattgaag
     5401 gaattgattg aaaccgcaag ggcaaagccc ggcaccctct cgtacggcta cggcggcaac
     5461 ggctcgacca tgcatctcgc gggcgagctg ttcaacagga tggcgaacgt caaggtgcag
     5521 ggcgtgccct ataagggcag cggcccggtg tcggccgacg tgctgggcgg ccaggtgccg
     5581 ctcggtgtcg tcgacgtgcc ctcggcgatc gccaatgtca aggcgggaaa gatccgtgcg
     5641 ctcgccgtga cgacgaagcg ccgcatcgcg gccgccccgg acgtcccaac gttcgaggaa
     5701 gccggactgc cgggctacga agcggtcggc tggttcggga cagtagcgcc ggcgggaact
     5761 ccggctaacg tagtaaaaaa actcaactct gaaatccggg cagcgctcac ggtacccgac
     5821 atccgcgagc gcgcgctggc cgccggcgcc gagccgtaca ccgacacgcc agaggagttc
     5881 gccgagttca tccgcgcgga aacgaagaag tgggcccagg tcatcaagga cgcgggcgtg
     5941 aagctccaat gacccgcagc cccgtcatcg acgtccacac ccacatgctc accaacgagt
     6001 gggtatcgct gctcgagcag cacggcgggc cgagctacac gctgaagccg gtggcgggcg
     6061 gcctcaaggc gatccacctc gacggcgcgc ccttcatgac ccccgttccc aacatgttcg
     6121 actgggaagc gcgcatcgcg aacatgaaca aggcgcgcgt cgacatcgcc gttacctccc
     6181 tcacctgccc gaacgtgttc tggggcagcg ccgaggcgag cttgaaggcg gcgcgcgtag
     6241 cgaacgacga catggcgagg gcgcagaaaa cctggcctga gcgcatccgc tggttcgcct
     6301 cgcttccgtg gcagcacgag aagcttgccc tcgaagagct agcccgtgct tgttcggctg
     6361 gagccgtcgg ggtcatggta ttggcaaaca tcggcggtaa agcgctgacc gatgaatctt
     6421 tcaagaaaat ctggcaagaa atcgatcggc gcgcgctgcc ggt
//
LOCUS       DATBSZ010000109        20089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1860037, whole genome shotgun sequence.
ACCESSION   DATBSZ010000109 DATBSZ010000000
VERSION     DATBSZ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20089
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860037"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="VIG70_07065"
     CDS             complement(<1..1255)
                     /locus_tag="VIG70_07065"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEY8250352.1"
                     /translation="MRRASVPIVSASLAGFALLAPAVHATGKHHRPPAEPVTCVELAT
                     DPDNGLLGAPGVKSVTSLIVPAAGANLAYCQVNILYGTNANQNINIRVGLPLNSLDGG
                     AGGVQGAWNGRTQGIGGGGCSGSVNVNAPVNAGYVGSGNDTGHVGGNCEPGVNEDGTY
                     NLQFINDFIRNGMKQQVLFSKSVAKTYYAAKPAYNYWNGCSTGGRQGYVVAQELGKEL
                     DGILANAPAIYWTRFQTAQMWGQIVMKDLVGAPIAAAKLSQATASAVAACDAADGVVD
                     GVIDDPRTCKFSATANVCGTATAPAANCLTLQEADAIDLIWDGPRNARGNRIWFGLDR
                     GTNLSGLNGTNPFALGVTQFHWDMHDRNFDWRTVTIDGYAQVAQEGSRNIADVTDTFG
                     DLDTFRRSGGKLLTFVGANDQLIMPR"
     gene            complement(1365..2624)
                     /locus_tag="VIG70_07070"
     CDS             complement(1365..2624)
                     /locus_tag="VIG70_07070"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY8250353.1"
                     /translation="MALIVQKFGGTSVGSVERIRNVARRIAKWKAAGHDLVVVPSAMA
                     GETNRLIGLAESVQPQPDARELDVVASTGEQVTVGLLSMALIGLGVKAKSYAGWQVKI
                     LTDSAFTKTRIISIDQQRIRDDLKAGHVVVVAGFQGVDGEGNITTLGRGGSDTSAVAL
                     AAALKAQECQIYTDVDGIYTTDPRLVPEARRLSTITFEEMLEMAGAGSKVLQIRSVEF
                     AGKYRVPTRVLSSLTDPALPIGEEARSGTLITFEEDPHMAMEKAVISGVAFNRDEAKI
                     TVHDVPDRPGIAYAILGPIADAHIDVDVIVQNVGHDGMTDLSFTVHRNDYAKTMKLLK
                     GQVQPHIKCRDVSGDERIAKVSIVGMGMRSHAGIAAQMFRTLAEEGINIQMISTSEIK
                     ITVVIEEKYTELAVRVLHKAFELENAG"
     gene            2720..3553
                     /locus_tag="VIG70_07075"
     CDS             2720..3553
                     /locus_tag="VIG70_07075"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HEY8250354.1"
                     /translation="MKRLLIAIYCLAALPLTGAAQPTHDYEFFPRDAPFRPPVADPAE
                     PRVFMSGLQVKRDAGSFSAAFLGVGVDFGLLRRSSARPDHGWQLSVFGSADSLFNLDL
                     PGDALVNTDYRFGLPVTWRNGPFSLRGRFYHQSSHLGDELILGGNAPRRVDLSFEAVD
                     LLVAWERSGWRLYGGASQVVRSSTDSYEGSGVHAGFDYVGPPVFFGQRLTAGLDVKWL
                     EQADWRSGVSAKAGIKIGSHTLEQRGITLLLEAYEGFAPFGQFFVEDLRYRGATLQFD
                     F"
     gene            complement(3550..4533)
                     /locus_tag="VIG70_07080"
     CDS             complement(3550..4533)
                     /locus_tag="VIG70_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8250355.1"
                     /translation="MTFLWPEFLWLLLAVPASIAGYLLLLKVKKKAAVRYPSLGLIRE
                     AAGKGLWWRRHLPPAMLVLGLAAMIAAIARPAAVITLPTSHETVILAIDVSGSMRAND
                     VEPSRIEAAQAAARTFVNEQPRSTRIGVVAFAGSAALVQPPTSNRTDVVAAIDQLQLQ
                     HATAVGSGLLVSLKAIFPELDFDLQKKPDLKPVPPGSYSSAAIVLLTDGQTTTGPDPV
                     DAARLAAERGVRVFTVGVGTDNGQILTGEGWSMRVRLDEDALKAIADLTRAEYFYAGN
                     AVDLKKVYQSLRSKLVMEKKQTEITALFSAIAAAIVLLSATLSMLWFNRIV"
     gene            complement(4535..5341)
                     /locus_tag="VIG70_07085"
     CDS             complement(4535..5341)
                     /locus_tag="VIG70_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8250356.1"
                     /translation="MKAKWRDLYKRHERPLAISAFIMLAAFAAASHVTKSPVAPAISQ
                     ERIDAAVARSLSEQPLPSPAAKVYEAVKGAIVLVRAIGDALETDEYLQKAAGTGVVVV
                     NRGTILTSLHVVAGAGRIRVVFADGLESDAKVVGQQPENDLAVLQAETVPDDLAAAVM
                     RPRADLQPGDTVVAVGFPFGIGPSVSAGVVSGLKREYRSPGGESHLSNLIQFDAAVNP
                     GSSGGPLLNAAGEVVGIVTAMLDPGGGGFAGIGFAVPVETAAAAAGTAPF"
     gene            complement(5615..6643)
                     /locus_tag="VIG70_07090"
     CDS             complement(5615..6643)
                     /locus_tag="VIG70_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8250357.1"
                     /translation="MTFLWPEMLWLMLALPLLAAAYVLVLRRKKKLALRYASLGLVRE
                     AMGVGQRVKRHIPPFLFFLALAAMLFAIARPQATVTLPTQHETVILAMDISGSMRATD
                     VEPNRLVAAQNAAKQFVAEQPENVRIGVVAFAGTATVAQAPTRNREDVVAAIDRFQLQ
                     RATAIGSAIIVSLATIFPEAGIDIGALTYGAETPKKGEKPAAQAHKPVPPGSYASAVI
                     ILLTDGQRTTGPDSVQAAKMAAERGVRIYTVGVGTPDGKIIGFEGWSMRVRLDEETLK
                     SVADITRGEYFYAGNALDLKKVYQALNAKLVLERKKTEVTALFAAIAALFAVVSAALS
                     LAWFHRIV"
     gene            complement(6640..7593)
                     /locus_tag="VIG70_07095"
     CDS             complement(6640..7593)
                     /locus_tag="VIG70_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8250358.1"
                     /translation="MSRLQARNPEAVLRRLEWTVIRRLDGLLQGDYRTLFRGFGLDLA
                     DLREYQYGDDVRHIDWNVTARLQTPYVREYHEDREVTAWFLLDLSSSLDFGSGEVRKR
                     GVSADFVAVLTRILTRHGNKVGALFYGEDVDAMMPARTGRRHVLQILHAMLARPAPTR
                     NAPTDLGELLAAGAHLARRRSLVFVVSDFFSVPGWEARLGQLAQRHEVVAVRLHDPLE
                     SSLPDLGMLVVQDAETGEQIFVDTHDRAFRKRFTAAAQRREAALRAAFRDAGIDALEL
                     STDDDLVDAIRRFADMRKRRSRLAGGAPLPKHLEVMGTNAS"
     gene            complement(7571..8563)
                     /locus_tag="VIG70_07100"
     CDS             complement(7571..8563)
                     /locus_tag="VIG70_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8250359.1"
                     /translation="MENVATLMEKILYEVKKVVVGQDHFLERVLVATLAQGHLLVEGV
                     PGLAKTLTVKTLAQTIRGSFRRIQFTPDLVPADLVGTRIYNQKTGEFHTSLGPVFCNL
                     LLADEINRAPAKVQSALLEVMQERQVTIAGETHHVPSPFLVMATQNPIETEGTYPLPE
                     AQVDRFMMKVLVDYPSEEEEFVIVERVTGVLAEAHGVATTDELGALQRECRRGYVEPS
                     LMQYAVRLAAATRDPERYGIKGLAKYINYGASPRASIHVIEGARALAFLRGRDYALPE
                     DVTDLVPDVFRHRLVLSYEALAEGLSPDELVKRVMQHIRQPAKPLETHVKVAGA"
     gene            complement(8572..9456)
                     /locus_tag="VIG70_07105"
     CDS             complement(8572..9456)
                     /locus_tag="VIG70_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8250360.1"
                     /translation="MKRAAVYSSSSRARAAAKPAVPANPPITFREWRKRHARGIKVGA
                     AVAGALVLGLFLWMLAPAKGLTLADIEVAVQKALESQQPALTAADAYESILPSVVHVR
                     GLPDAPDADVPSERESQSGTGVVIVDTGIILTNLHVVHGAKSIKVVFHDGLESDAIVT
                     GVQPEHDLAVLQAKIIPDDLYAATLRSTQGLRPGEPVVAVGFPFGIGPSVSAGVISGL
                     GREYQTLDGKRVLTNLIQFDAAVNPGNSGGPLITLEGEVIGIVTGLLNPSEQRVFIGI
                     GFAVPIENAAGAVGLSPF"
     gene            complement(9493..9580)
                     /locus_tag="VIG70_07110"
     tRNA            complement(9493..9580)
                     /locus_tag="VIG70_07110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9544..9546),aa:Ser,seq:tga)
     gene            9697..10428
                     /locus_tag="VIG70_07115"
     CDS             9697..10428
                     /locus_tag="VIG70_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEY8250361.1"
                     /translation="MQPELKPVRTDDQGFGRGTRYEPVQDVAVTDQGQKVLRNTYMLL
                     ALTMVPTVIGAWIGMATSSIIVASPIISTLVMLAAVIGLQFGIAANRNSGVGVALLLL
                     MTGLLGWWLGPILNVALSMKNGMALVGYAAVGTGVIFFAMGALAQTIKRDLSFMGKFL
                     FVGMIALLIAMIANMFLQIPALALTISTLVIVVFSLFLLYDLQRIMRGGETNYVMATT
                     GVYMSLFNIFANLLHLLLALAGDRD"
     gene            complement(10469..11818)
                     /gene="rlmD"
                     /locus_tag="VIG70_07120"
     CDS             complement(10469..11818)
                     /gene="rlmD"
                     /locus_tag="VIG70_07120"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEY8250362.1"
                     /translation="MQHAARRSRETSGAALAGVPVALENLILNIDALDAEGRGVARNA
                     EGKVVFVEGALPSERATVRLFKEKRKFDLGRATSIVERSAGRREPRCPHFGVCGGCAT
                     QHADERTQMAAKQRWLEDCLERIGKVKPETLLPIVYGPEWGYRHRARLSVRHVQSRGA
                     MVGFRERRSTHVADMHECHVLPEGISALISPLRALVENLSIRERLPQIEVAVGDNAVV
                     LVFRHLLPLTDADHLALKDFSERHQIHVWLQPAGPDSAHPFHPEASELHYDLPEFGVR
                     IRFRPTEFTQVNFAMNRVLVSRALRLLDPQPGERIADLFCGLGNFSLPLAAKGAQVVG
                     FEGSAGLVERARQNAAANGLVAQFEAVDLFKHGIAAFGPFDKLLIDPPREGAVEIVKS
                     LPETWPRRVVYVSCDPATLARDAAVLVHTKGWRLAAAGVVNMFPHTAHVESIALFEK"
     gene            complement(11740..12513)
                     /locus_tag="VIG70_07125"
     CDS             complement(11740..12513)
                     /locus_tag="VIG70_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY8250363.1"
                     /translation="MTPVLAFDIETIPDVAGIRRLYDLPADLPDQDVAELAAQKRRVQ
                     TGNDFLPTYLHRVVVISCVLRDDDGVRVFSIGEPEANEAAAIKRFFEGIDKYVPQIVS
                     WNGRGFDLPVLVNRALQHGVGAACFWDTGRDNQDFRYSNYINRFHERHVDLMDVLSLY
                     GGRGSPLDDVARLAGLPGKVGIGGNAVWESYRRGELAAIRNYCEADCVNTYLLFLRFQ
                     QMRCVFDAARYAEECSMLRAALEKRPEPHWREFLSLWKT"
     gene            complement(12529..12720)
                     /locus_tag="VIG70_07130"
     CDS             complement(12529..12720)
                     /locus_tag="VIG70_07130"
                     /inference="COORDINATES: protein motif:HMM:NF022823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 family protein"
                     /protein_id="HEY8250364.1"
                     /translation="MKIVAIVLVVLGIGLAFWGYRLSGGVGAQLSEAVTGSPTDKIMA
                     FYIGGAASLAVGLFLLFRK"
     gene            complement(12746..13633)
                     /gene="cysM"
                     /locus_tag="VIG70_07135"
     CDS             complement(12746..13633)
                     /gene="cysM"
                     /locus_tag="VIG70_07135"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEY8250365.1"
                     /translation="MKRTVEDFIGNTPLVRLQRLPGSDKNILLAKLEGDNPAGSVKDR
                     PAISMIRRAEKRGRIKPGDTLVEPTSGNTGIALAMAAAIRGYRLVLIMPEHLSAERRQ
                     TMRAYGAEIILTPQAGGMEAARDLAERMVREKKGTMLDQFANPDNPLVHYETTGPEIW
                     RDTDGRITHFVSSMGTTGTIMGCSRFFKEKNAAIQVIGCQPQEGSQIPGIRKWPEAYL
                     PKIYDARRVDRLEYVSQAEAEEMTRRLAREEGIFAGISSGGAMHVALRVSKELKNAVI
                     VTIVCDRGDRYLTTGVFPD"
     gene            complement(13639..14622)
                     /gene="rfaD"
                     /locus_tag="VIG70_07140"
     CDS             complement(13639..14622)
                     /gene="rfaD"
                     /locus_tag="VIG70_07140"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEY8250366.1"
                     /translation="MTYTIVTGAAGFIGSKLVEGLNRRGITEVIAVDNLQHSDKFRNL
                     AGCEIADYVDQAEFIANLERYEGAVEAVFHQGACSDTMESDGRYMMDNNYGYSKRLLE
                     WCQDEEVPLIYASSASVYGAGPEFREERRCEKPLNVYGYSKFLFDQYVRGTLETRTSQ
                     VVGLRYFNVYGPNEAHKGRMASVALHAYQQLHAEGRVKLFVGCDGYRDGEQRRDFIYV
                     DDVVAVNLWFLERRDVSGVFNCGTGKAQSFNRLAAAVVNAVHGTQAMLQDLVAKRLIE
                     YVPFPPALVGKYQSFTEADLSRLRAAGYPGEFKNVEQGVAAYMAELKKQEQ"
     gene            complement(14619..15506)
                     /gene="rfaE1"
                     /locus_tag="VIG70_07145"
     CDS             complement(14619..15506)
                     /gene="rfaE1"
                     /locus_tag="VIG70_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEY8250367.1"
                     /translation="MLDRYWFGEVSRISPEAPVPVVLVNEKSEVLKLGGAANVAWNCK
                     ELGARTRLLSVVGTDEPGRSLEKLLKKMGVEASLHHDRSLSTTQKLRVIGRRQQLLRI
                     DFERPPSREVLASKLEDFKRALPESDVIILSDYGKGGLHHIAEMIRSARRAGKRVLVD
                     PKGDDYARYKGASIITPNLAELREVVGTWKDEKDLKARAEALRDKLKLEALLLTRGGD
                     GMTLFGKKVMSIKAEAREVFDVTGAGDTVIATLAVMLAAGATLEAGVRIANRAAGIVV
                     GKLGTAAATHAELFGKGSK"
     gene            complement(15545..16855)
                     /locus_tag="VIG70_07150"
     CDS             complement(15545..16855)
                     /locus_tag="VIG70_07150"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY8250368.1"
                     /translation="MNVTIIGTGYVGLVTGACLADAGNNVFCLDVDEQKIRALNAGRI
                     PIYEPGLEPIVQRNEAAGRLRFSTDVAASVAHGELQLIAVGTPPDEDGSADMQYVLAA
                     ARAIGKHMKSYKVIATKSTVPVGTADRIAEAVKKETDVPFSVVSNPEFLKEGAAIEDF
                     MRPDRIIVGSEDPRAIALLRQLYAPFQRNRERIIVMGVRSAELTKYAANAMLATRISF
                     MNEIANIAEALGADVEEVRHGIGADPRIGTAFLYPGAGYGGSCFPKDVRALQRTASEA
                     KRELRVVAAVEAVNEAQKHVVAAKIKRRFGDRLNGKCFAVWGLAFKANTDDMREASSR
                     TLISDLVGAGARVRAYDPAAGHEGKRLYRDSPGIEIVDSALAALEGADALAVVTEWRE
                     FRSPDFAAIKARLKTPAIFDGRNLYDPSVLKGLGFEYFPIGRKS"
     gene            complement(16852..18015)
                     /gene="lapB"
                     /locus_tag="VIG70_07155"
     CDS             complement(16852..18015)
                     /gene="lapB"
                     /locus_tag="VIG70_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEY8250369.1"
                     /translation="MEFEYWWLLGFPLFFGLGWIAARIDIKQIVSESRALPRSYFKGL
                     NFLLNEQPDKAIEAFIEVVKVDPETIELHFALGSLFRRRGEYDRAIRMHQNLLERADL
                     AGEQKLQAQMELGQDYLKAGILDRAEEVFARLEKTTQGTAARRFLLEIYEQEKDWPRA
                     IEMTKQVETDPRALAQYLCELAASESAQSHPDAARRHLDAALEANRKCVRASLLWGDL
                     ERSANNIDRAVEHWMRVESQDPAYLALVAQRLVEAYRDAGRAEEGLTLLAGYLERYPS
                     LDLLDNVFQQTLEAKGPEQAYAMVRDELRRNPTLLGLDRLLEAQIIAAGSPDKRRDLE
                     LVRNLVHGHTRRLARYRCESCGFKARQFHWHCPACGGWETYPPRRTEEFDLTP"
     gene            complement(18018..18287)
                     /locus_tag="VIG70_07160"
     CDS             complement(18018..18287)
                     /locus_tag="VIG70_07160"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEY8250370.1"
                     /translation="MRIVTWTIRVGVFLLLIAFAAKNVEPVVLRFYFDLALQAPLVLL
                     LLGAFALGALFGVLALLTTVLRQRRQISLLKRPLEPVVPPIPPLV"
     gene            complement(18316..18648)
                     /locus_tag="VIG70_07165"
     CDS             complement(18316..18648)
                     /locus_tag="VIG70_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEY8250371.1"
                     /translation="MTKSELIARLAQRYPQLVAKDAEYAVKMILDAMTQSLLSGHRIE
                     IRGFGSFGLNYRPPRTGRNPKSGEKVQVPEKYVPHFKAGKELRERVDSATPQSSSAPQ
                     PRASQPVR"
     gene            complement(18676..>20089)
                     /gene="rpsA"
                     /locus_tag="VIG70_07170"
     CDS             complement(18676..>20089)
                     /gene="rpsA"
                     /locus_tag="VIG70_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY8250372.1"
                     /translation="WIGLEKALESGEKIKGVITGKVKGGLTVMANGIRAFLPGSLVDL
                     RPVKDTTPYEGKEMEFKVIKLDRKRNNVVVSRRAVLEETAGAEREQLLASLQEGATVK
                     GVVKNITDYGAFVDLGGIDGLLHITDLAWRRVKHPSEVLNVGDEVTAKVLKFDSEKNR
                     VSLGLKQLGEDPWVGIARRYPQGTRLFGKVTNLTDYGAFVEVESGIEGLVHVSEMDWT
                     NKNVHPSKVVQVGDEVEVMILEIDEERRRISLGMKQCMPNPWEDFAREYKKSDKVKGQ
                     IKSITDFGVFVGLPGGIDGLVHLSDLSWSAPGEEAVKNFKKGEEVEAVVLSIDIERER
                     ISLGIKQLEGDPFTNFIAGHEKNSVVTGAVKSVDAKGAVVDIGGVEGYLRASEFARDR
                     IDDLTKHLKEGDSVQAMIINVDRKNRSINLSVKAKDLAEETDAMKQLRTEQAAASAGA
                     TNLGALLRAKLDKKDGNPQQ"
BASE COUNT         3112 a         6733 c         6737 g         3507 t
ORIGIN      
        1 cgcgcggcat gatgagctgg tcgttcgcgc cgacgaaggt gagcagcttg ccgccgctgc
       61 gacggaaggt gtcgagatcg ccgaaggtgt cggtgacgtc ggcgatgttg cgcgagcctt
      121 cctgcgccac ctgcgcgtac ccgtcgatcg tcacggtgcg ccagtcgaag ttgcggtcgt
      181 gcatgtccca gtggaactgg gtcacgccga gcgcgaacgg gttggtgccg ttcaggccgc
      241 tcaggttggt cccgcgatcg agcccgaacc agatcctgtt gccgcgcgcg ttgcgcggcc
      301 catcccagat cagatcgatg gcgtcggcct cctggagcgt caggcagttc gccgccgggg
      361 cggtcgccgt gccgcagacg tttgccgttg cgctgaactt gcacgtccgc ggatcgtcga
      421 tcacgccgtc gaccacgccg tcggctgcat cgcaggccgc gaccgccgag gcggtggcct
      481 gggagagctt ggcagcggcg atcggggcgc cgacgaggtc cttcatcacg atctggcccc
      541 acatttgcgc ggtctggaat cgtgtccagt agatcgccgg ggcgttcgcg agaatgccgt
      601 cgagctcctt gccgagctcc tgcgccacca cgtagccctg gcgcccgccg gtcgagcagc
      661 cgttccagta gttgtaggcg ggcttggctg cgtaataggt tttcgccacc gacttggaga
      721 agagcacctg ctgcttcatc ccgttgcgga tgaagtcatt gatgaactgc aggttgtacg
      781 tcccgtcctc gttgacgccg ggctcgcagt tgcccccgac gtggccagta tcgttgcccg
      841 atcccacgta gcccgcgttg accggcgcgt tgacgttcac gcttccggaa cagccgccgc
      901 cgccgatccc ctgcgtcctg ccgttccagg ctccctgcac accgcccgcg ccgccgtcca
      961 gcgagttgag cggcaggccg acgcggatgt tgatgttctg gttggcgttc gtgccgtaca
     1021 ggatgttcac ctggcagtag gcgaggttcg ctccggccgc cgggacgatg aggctcgtga
     1081 ccgacttgac gcccggggcg ccgagcaggc cgttatcggg atcggtggca agctcgacgc
     1141 aggtcaccgg ctcggccggc gggcggtggt gcttgccggt ggcgtgcact gcaggtgcga
     1201 gtaaagcgaa accggcgaga ctggccgaga cgattggcac ggatgcgcgg cgcatgggac
     1261 tcctcctcta gaggcagacc ctagtaagcg cggttttgcc tgtcaatcaa aacggtttgc
     1321 accatagtgc atcccacggc cgatccatga gcaacgctta tgaatcagcc ggcgttctcg
     1381 agctcgaaag ccttgtgcag cacgcgcacg gcgagctcgg tgtacttctc ctcgatgacc
     1441 acggtgatct tgatctcgga ggtggagatc atctggatgt tgatgccctc ctcggcgagg
     1501 gtgcggaaca tctgcgccgc gatgccggcg tgcgagcgca tgcccatgcc gacgatcgac
     1561 accttggcga tgcgctcgtc gccgctcacg tcgcggcact tgatgtgcgg ctggacctgc
     1621 cccttcagga gcttcatcgt cttggcgtag tcgttgcggt gcacggtgaa cgacaggtcg
     1681 gtcatgccgt cgtggccgac gttctgcacg atgacgtcga cgtcgatgtg ggcatcggcg
     1741 atcgggccga ggatggcata ggcgatgccg ggacggtcgg gaacgtcgtg caccgtgatc
     1801 ttggcctcgt cgcggttgaa cgcgacgccg gagatgactg ctttttccat cgccatgtgc
     1861 gggtcttcct cgaaggtgat cagcgtgccg gagcgcgcct cctcgccgat cggcagcgcc
     1921 gggtcggtca ggctggagag cacgcgcgtc ggcacgcggt acttgccggc gaactcgacc
     1981 gagcggatct gcagcacctt ggagccggcg cccgccatct cgagcatctc ctcgaacgtg
     2041 atggtgctca ggcgccgcgc ctcgggcacg agccgcgggt cggtggtgta gatgccgtcg
     2101 acgtcggtgt agatctggca ctcctgcgct ttcagggccg cggcgagcgc caccgcggag
     2161 gtgtcggagc caccgcggcc gagcgtggtg atgttcccct ctccgtccac gccctggaag
     2221 cccgctacga ccacgacgtg gcccgctttc agatcgtccc ggattcgctg ctgatcgatg
     2281 gaaatgattc gggtcttggt gaacgccgaa tccgtcagga tcttcacctg ccagccagca
     2341 tagctcttcg ccttcacgcc gagcccgatc agcgccatcg aaagcaggcc gacggtcacc
     2401 tgctcgccgg tcgaggcgac gacgtcgagc tcgcgcgcgt cgggttgcgg ctgcacgctc
     2461 tcggcgagcc cgatcaggcg gttggtctcg cccgccatcg ccgaagggac gaccacgagg
     2521 tcgtggcccg ccgccttcca cttcgcgatg cggcgcgcga cgttgcggat tcgctcgacc
     2581 gaaccgacgg aggtgccgcc gaacttctga acgattaacg ccatggaaaa acgaggaatt
     2641 ttaccggata ccgctatttc atccggcccc gagcgccgct agacggcaag cgctctcttg
     2701 ccggactctg ctggctcctg tgaagagact cctgatcgcc atctattgcc tcgcggcgct
     2761 ccccttgacc ggcgctgcgc agccgacgca cgactacgag ttcttcccgc gcgacgcccc
     2821 attccgcccg ccggtggcgg accccgccga gccgcgcgta ttcatgagcg ggctccaggt
     2881 gaagcgcgat gccggcagtt tcagcgcggc gtttctcggc gtcggggtgg acttcggcct
     2941 gctacgccgc agtagcgcga gaccggacca tggctggcag ctgagcgtgt tcggctcggc
     3001 ggactcgctc ttcaacctgg atttgccggg cgacgcgctg gtcaacaccg actaccgctt
     3061 cggcctgcct gttacctggc gcaacgggcc cttctccctg cgcgggcgtt tctaccacca
     3121 gagctcgcat cttggcgacg agctgattct cggcggcaac gcgccgcggc gcgtcgacct
     3181 cagcttcgag gcggtcgacc tgctcgtcgc ctgggagcgc tcgggctggc gcctgtacgg
     3241 aggcgcgagc caggttgtac gcagcagcac cgacagctac gagggcagcg gcgtgcacgc
     3301 cggcttcgac tatgtcggcc caccggtgtt tttcggccag cggctgaccg cgggcctgga
     3361 cgtgaagtgg ctcgagcagg ccgactggcg ctcgggcgtc agcgcgaagg ccgggatcaa
     3421 gatcggtagc catacgctcg agcagcgcgg gatcaccttg ctgctggagg cctacgaggg
     3481 cttcgccccg ttcgggcagt tcttcgtcga ggacctcagg taccgtggcg cgacgctaca
     3541 gttcgatttc tagacgattc tgttaaacca aagcatcgac agcgtcgcgg acagaaggac
     3601 aatcgccgcc gcgatcgcag agaacaacgc ggtgatttcg gtctgcttct tctccatcac
     3661 cagcttcgag cgcagcgact ggtacacctt cttcaggtcc accgcgttgc cggcgtagaa
     3721 gtactcggcg cgcgtcaggt cggcgatcgc cttcagcgcg tcctcgtcga ggcgcacgcg
     3781 catcgaccag ccctcgccgg tgaggatctg gccgttgtcg gtgccgacgc cgaccgtgaa
     3841 cacgcgcacg ccgcgctcgg cggcgaggcg cgccgcatcg accgggtctg gcccggtggt
     3901 ggtctggccg tcggtgagca gcacgatcgc cgccgaggaa taggaccccg gcggcaccgg
     3961 cttgaggtcc ggttttttct gcagatcgaa atcgagctcc ggaaagatcg ccttcagcga
     4021 cacgaggagc ccgctgccca cggcagtggc gtgctggagc tgcagctggt cgatcgcggc
     4081 gaccacgtcg gtgcggttgc tggtcggggg ctggaccagg gcggcgctgc cggcgaacgc
     4141 caccacgccg atgcgcgtgc tgcgcggctg ctcgttgacg aacgtgcgcg ccgccgcttg
     4201 cgccgcctcg atccgcgagg gctcgacgtc gttcgcccgc atgctccccg agacgtcgat
     4261 cgccaggatc acggtctcgt ggctcgtggg cagcgtgatc accgcggccg gacgcgcgat
     4321 cgcggcgatc atggcggcga gcccgaggac cagcatcgcc ggcggcaggt ggcggcgcca
     4381 ccacaggcct ttgcccgcgg cttctcgaat caatccaagg ctcgggtagc gcaccgctgc
     4441 tttcttcttc accttcaata gcaggagata accggcgatg ctcgccggca cggcgagcag
     4501 aagccacagg aattcgggcc agaggaaagt catgtcagaa cggcgccgtg ccggcggccg
     4561 cagcggcggt ctcgaccggc accgcgaagc cgatgccggc gaagccgccg ccgcccggat
     4621 cgagcatcgc ggtgacgatg ccgaccactt cgccggcggc gttgaggagc ggcccgcccg
     4681 agctgcccgg gttgaccgca gcatcgaact ggatgaggtt cgacagatgg gactcgccgc
     4741 ccggcgagcg gtactcacgc ttcaggccgg acacgacgcc ggcgctgacc gaaggcccga
     4801 tgccgaacgg gaagccgacc gcgaccaccg tatcgcccgg ctgcaggtcg gcgcgcggcc
     4861 gcatcaccgc cgcggcgaga tcgtcgggca cggtctcggc ctggaggacg gcgagatcgt
     4921 tctcgggctg ctggccgacg accttcgcgt cggattcgag gccgtcggcg aacaccaccc
     4981 ggatccggcc cgcgcctgcg accacgtgca ggctggtaag aatcgtgccg cgattgacga
     5041 ccaccacgcc cgtgccggct gccttctgca ggtattcgtc ggtctcgagc gcgtcgccga
     5101 tcgcgcgcac cagcacgatc gcgcctttca ccgcctcgta gaccttcgcc gcgggcgacg
     5161 gtaaaggctg ctcggaaagc gatcgcgcga ccgcggcgtc gattctctcc tgcgagatcg
     5221 ccggcgccac cggcgacttc gtcacatgcg acgcggcggc gaacgctgca agcatgatga
     5281 aagctgagat cgccagcggg cgctcatggc gcttgtagag atcgcgccac ttcgccttca
     5341 caggcgcttc gacaggtttt tccggactgg gcgattcagg acgagcgccg acgggagaac
     5401 gaggggaatt gctatagagc gacacgcgtc tcatctggcg ttgaccacat tctggtcgcg
     5461 ccagttcttc gccgtgcagt tcgggctatg gtccggtcgt ctagggaatc cctgaaaaag
     5521 cagcagttgg tcgttcccgc gaacgcggga acctatgtgc ctcaaaaaca aattaggccc
     5581 ccgccttcgc gggggcgact ccttcagacc ttccctaaac gattctgtga aaccaggcga
     5641 gagacaacgc cgcggaaacg acggcgaaca gcgcggcaat cgcagcgaac agcgcggtga
     5701 cttcggtctt cttgcgctcg agcacaagct tggcgttgag cgcctggtag accttcttga
     5761 gatccagcgc gttgccggcg tagaaatact cgccgcgcgt gatgtcggcg accgacttca
     5821 gcgtctcctc gtcgagccgc acgcgcatcg accagccctc gaagccgatg atcttgccgt
     5881 cgggcgtgcc gacgccgacg gtgtagatgc gcacgccgcg ctcggcggcc atcttcgcgg
     5941 cctggaccga gtccgggccg gtggtgcgct ggccgtcggt gagcaggatg atgaccgcag
     6001 aagcgtacga gcccggcggc accggcttgt gcgcctgcgc ggcgggcttc tcgcccttct
     6061 tcggggtctc cgcgccgtag gtgagcgctc cgatgtcgat tcccgcttcc gggaagatgg
     6121 tggcgagcga cacgatgatc gcgctgccga tggcggtggc gcgctgcagc tggaagcggt
     6181 cgatcgcggc gaccacgtcc tcgcggttgc gcgtcggcgc ctgcgccacg gtcgcggtgc
     6241 cggcgaaggc gaccacgccg atgcgcacgt tctccggctg ctcggcgacg aactgcttcg
     6301 cggcgttctg cgccgcgacc agccggttcg gctcgacgtc ggtggcgcgc atgctgccgg
     6361 agatgtccat cgccaggatg accgtttcgt gctgcgtggg caacgtgacc gtggcctgcg
     6421 ggcgcgcgat cgcgaacagc atcgccgcaa gcgcgaggaa gaacaggaac ggcgggatgt
     6481 ggcgcttgac gcgctgtccg acgcccatcg cctcgcgcac cagcccgagg ctcgcgtagc
     6541 gcagcgcgag ctttttcttc cttctcaaga ccaggacata ggccgcagcc agcagcggca
     6601 gcgccagcat cagccaaagc atctcgggcc ataagaaggt catgaggcgt ttgtccccat
     6661 gacttccaag tgcttgggca gcggggcgcc gccggcgagc cggctgcggc gcttgcgcat
     6721 gtcggcgaag cggcgaatcg cgtccaccag gtcatcgtcg gtcgagagct cgagcgcgtc
     6781 gatgccggcg tcgcggaacg cggcgcgcag cgcggcctcg cggcgctgcg ccgctgccgt
     6841 gaagcgcttg cggaacgcgc ggtcgtgcgt atcgacgaag atctgctcgc cggtctccgc
     6901 gtcctgcacc accagcatgc cgaggtcggg caggctcgac tccagcgggt catgcaggcg
     6961 cacggcgacg acctcgtggc gctgcgcgag ctgtccgagc cgcgcctccc agcccggcac
     7021 gctgaagaaa tcggacacca cgaacacgag cgatcgccgc cgcgccaagt gcgcacccgc
     7081 ggcgaggagc tcgccgaggt cggtgggcgc attgcgcgtg ggcgcggggc gcgccagcat
     7141 ggcgtgcagg atctgcagca cgtggcgccg gccggtgcgt gccggcatca tcgcgtcgac
     7201 gtcctcgccg tagaacagcg cgcccacctt gttgccatgg cgcgtgagga tgcgcgtgag
     7261 caccgcgacg aaatcggccg acacgccgcg cttgcgcact tcgcccgagc cgaaatccag
     7321 cgacgaggac agatccagca ggaaccaggc ggtgacctcg cggtcctcgt ggtattcgcg
     7381 cacgtagggc gtctgcagcc gcgcggtcac gttccagtcg atgtggcgca cgtcgtcgcc
     7441 gtactggtat tcgcgcaggt cggcgaggtc caggccgaag ccgcggaaca gcgtgcggta
     7501 gtcgccctgc agcagcccgt cgagccgccg gatcaccgtc cactccaggc ggcgcaggac
     7561 cgcctcggga ttacgcgcct gcaaccttga catgagtctc gagcggcttg gccggctggc
     7621 ggatgtgctg catcacgcgc ttgacgagct cgtcgggcga aagtccctcg gcgagcgctt
     7681 cgtaggacag caccaggcga tggcggaaca cgtcgggcac gagatcggtc acgtcctccg
     7741 gcagcgcata gtcgcgcccg cgcaggaacg cgagcgcgcg cgcgccctcg atgacgtgga
     7801 tcgaggcgcg cgggctcgcg ccgtagttga tgtacttcgc gaggcccttg atgccgtagc
     7861 gctcggggtc gcgcgtcgcc gcggcgaggc gcaccgcgta ctgcatgagg gagggctcga
     7921 cgtagcccct gcggcattcg cgctgcagcg cgccgagctc gtcggtggtc gccacgccgt
     7981 gcgcctcggc cagcacgccg gtcacgcgct cgacgatgac gaactcctcc tcctcgcttg
     8041 ggtagtcgac cagcaccttc atcatgaagc ggtcgacctg cgcctcgggc agcgggtagg
     8101 tgccctcggt ctcgatcggg ttctgcgtcg ccatcaccag gaacggcgag ggcacgtggt
     8161 gggtctcgcc cgcgatcgtc acctggcgct cctgcatcac ctcgagcagc gcgctctgca
     8221 cctttgccgg cgcgcggttg atctcgtcgg cgagcagcag gttgcagaac accggcccga
     8281 gcgaagtgtg gaactcgccg gtcttctggt tgtagatgcg cgtgccgacg aggtcggcgg
     8341 ggaccaggtc gggcgtgaac tggatgcggc ggaagctgcc gcggatggtc tgcgcgaggg
     8401 tcttcacggt gagcgtcttc gcgaggcccg gcacgccttc gaccagcagg tggccctgcg
     8461 cgagcgtcgc caccagcacg cgctcgagga agtgatcctg gccgaccacg accttcttca
     8521 cctcgtagag gatcttctcc atcaaggtcg cgacgttttc catgggttcc tttagaaagg
     8581 ggagagaccg accgcgccgg cggcgttctc gatcgggacg gcaaaaccga tgccgatgaa
     8641 cacgcgctgc tcgctcgggt tgaggagccc agtgacgata ccgatcacct cgccctcgag
     8701 cgtgatcagc ggcccgccgg agttgcccgg gttcaccgcg gcgtcgaact ggatgaggtt
     8761 ggtcagcacg cgcttgccgt cgagcgtctg gtactcgcgc ccgaggcccg agatcacgcc
     8821 tgcagacacc gacgggccga tgccgaaagg aaagcccact gccacgaccg gctcgccggg
     8881 ccgcaggccc tgcgtcgagc gcagcgttgc cgcataaaga tcgtcgggga tgatcttcgc
     8941 ctgcagcacc gcgagatcgt gctccggctg cacgccggtc acgatcgcgt cggactccag
     9001 gccatcgtgg aacacgacct tgatgctctt cgcgccgtgc accacgtgga ggttcgtcag
     9061 gatgatgccg gtgtcgacga tcaccacgcc ggtgccgctc tggctctcgc gctccgaggg
     9121 cacgtcggcg tcgggcgcat ccggcagccc gcgtacgtgc accaccgaag gcaggatgct
     9181 ctcgtaggcg tcggccgcgg tcagcgccgg ctgctgcgat tcgagcgcct tctggaccgc
     9241 gacctcgatg tcggcaaggg tgagtccctt ggccggcgcc aacatccaga ggaagagtcc
     9301 caggaccagc gcgcccgcca ccgcggcgcc aaccttgatg ccgcgggcgt ggcgcttgcg
     9361 ccactcgcga aacgtaatgg gaggattggc cggcactgcg ggcttggccg ctgcccgcgc
     9421 ccggctcgaa ctgctgtaga ccgccgctcg tttcatgcct gaatccgttg ggattctatg
     9481 cccacatttg cctggcggag agtgagggat tcgaaccccc gagccctcgc gggctaacgg
     9541 ttttcaagac cgccgcattc gaccgctctg ccaactctcc ggcttgaaaa acccctgtaa
     9601 cgcccacatc ttaccttttg tctccgctcc ggctgttgca acttgggggg gctttggtag
     9661 tcttagtcgt tacatacata aagacttgga ggaaggatgc aacccgaact gaaacccgtt
     9721 cgcaccgacg atcagggctt tggccgtggc acccgctacg agccggtcca ggacgtcgca
     9781 gtcacggacc agggccagaa ggtcctgcgg aacacctata tgttgctggc gctgaccatg
     9841 gtgccgacgg tgatcggcgc ctggatcggc atggcgacga gcagcatcat cgtggcgagc
     9901 ccgatcatca gcacgctggt gatgctcgct gcggtcatcg gcctgcaatt cggcatcgcg
     9961 gccaaccgca acagcggcgt gggggtggcg ctgctgctcc tgatgacggg gctgctcggc
    10021 tggtggctcg ggccgatcct gaacgtcgcg ctctccatga agaacggcat ggcgctggtc
    10081 ggctacgccg cggtcggcac cggggtgatc ttcttcgcca tgggcgcgct cgcgcagacg
    10141 atcaagcggg atctgagctt catgggcaag ttcctgttcg tcggcatgat cgcgctcctg
    10201 atcgcgatga tcgccaacat gttcctgcag attccggcgc tcgcgctcac catctccacc
    10261 ctggtgatcg tggtgttctc gctgttcctg ctctacgacc tgcagcgcat catgcgcggc
    10321 ggcgagacca actacgtgat ggcgacgacc ggcgtctaca tgagcctgtt caacatcttc
    10381 gccaacctgc tgcacctgct cctggcgctg gcaggcgacc gcgactaagc tgcttgcacc
    10441 ttgagaaagg cgccttcggg cgcctttttt atttctcgaa cagcgcgatg ctttcgacgt
    10501 gcgcggtgtg ggggaacatg ttgaccacgc ccgcagcggc cagccgccag cctttcgtat
    10561 gcaccaggac ggcggcgtcc cgggcaaggg tcgccgggtc gcacgagacg tagaccacgc
    10621 gccgcggcca ggtttcgggg agcgacttca cgatttccac ggcaccttcg cgcggcgggt
    10681 cgatcagcag cttgtcgaac ggcccgaacg ccgcgatgcc gtgcttgaag agatccaccg
    10741 cctcgaactg cgccaccagg ccgttggccg ctgcgttctg ccgggcgcgc tccacgagac
    10801 cggcgctgcc ttcgaaaccc acgacctgcg cgcctttcgc ggcgagcggc agagaaaaat
    10861 tgcccaggcc gcagaaaagg tccgcgatgc gctcgcccgg ctgcggatcg agcagccgca
    10921 gcgcgcgcga gacgagcacg cggttcatcg cgaagttcac ctgcgtgaac tcggtcgggc
    10981 gaaaccggat gcgcacgccg aactccggca ggtcgtaatg cagctcgctc gcctcggggt
    11041 ggaacgggtg cgcgctgtcg gggccggcgg gctgcagcca gacatggatt tggtgtcttt
    11101 cggagaagtc tttcagcgcg agatggtcgg cgtccgtcaa cggcaacaaa tgccggaaca
    11161 ccagcaccac ggcgttgtcg ccgacggcga cttcaatctg cggcagccgc tcgcggatgg
    11221 acagattttc caccagagcg cgcaacggag aaatcaacgc gctgattccc tcgggcagca
    11281 cgtggcattc gtgcatgtcg gccacgtgcg tcgagcggcg ctcgcggaag cccaccatcg
    11341 cgccccggct ctgcacgtgg cgcaccgaga gccgcgcgcg gtgccgatag ccccattcgg
    11401 gaccgtagac gatcggcagc agcgtctccg gcttcacctt gccgatgcgc tcgaggcagt
    11461 cttccagcca gcgttgcttt gccgccatct gggtgcgctc gtcggcatgc tgcgtggcgc
    11521 aaccgccgca gacgccgaaa tgcgggcagc gcggctcgcg ccggcccgcg gagcgctcga
    11581 cgatggaggt cgcgcggccg aggtcgaact tgcgcttctc cttgaacaag cggaccgtcg
    11641 cgcgttcgga gggcaacgcg ccctcgacga acaccacctt gccctccgca ttgcgggcga
    11701 cgccgcgccc ttcggcgtcg agcgcgtcga tgttgagaat caggttttcc agagcgacag
    11761 gaactcccgc cagtgcggct ccggacgttt ctcgagagcg gcgcgcagca tgctgcactc
    11821 ctcggcgtag cgcgcggcgt cgaacacgca gcgcatctgc tggaagcgca ggaagagaag
    11881 gtaggtgttg acgcaatccg cctcgcagta gttgcggatc gcggcgagct cgccgcggcg
    11941 gtagctctcc cacaccgcgt tgccgccgat gccgaccttg ccgggaaggc ccgcgagcct
    12001 ggcgacgtcg tcgagcggcg agccgcgccc gccgtagagc gagagcacgt ccatgagatc
    12061 gacgtggcgc tcgtggaaac ggttgatgta gttgctgtag cggaagtcct ggttgtcgcg
    12121 gccggtgtcc cagaagcacg ccgcgccgac gccgtgctgc agtgcgcggt tcaccagcac
    12181 cggcaggtcg aagccgcgtc cgttccacga cacgatctgc ggcacgtact tgtcgatccc
    12241 ctcgaagaag cgcttgatcg cggcagcctc gttggcctcg ggctcgccga tcgagaacac
    12301 gcgcacgccg tcgtcgtcgc gcagcacgca ggagatgacc acgacgcggt gcagataggt
    12361 cggcaggaag tcgttgccgg tctgcacgcg gcgcttctgc gccgcaagct cggcgacgtc
    12421 ctggtcgggc agatccgccg gcaggtcgta cagccggcgg atgcccgcga cgtcggggat
    12481 ggtctcgatg tcgaacgcga ggaccggcgt catctcggct gggtcgccct atttcctgaa
    12541 cagcaggaac aggccgacgg caaggctggc tgcgccgccg atataaaaag ccatgatctt
    12601 gtcggtcggc gaacccgtga ctgcctcgct cagttgtgcg ccgacgccgc cggacaaccg
    12661 gtacccccag aacgccaggc cgatgcccag gacgaccagg acgattgcaa cgatcttcat
    12721 tgagttcctc ccatggtggg atgcgtcagt cggggaacac cccggtggtc agatagcggt
    12781 cgccgcggtc gcagacgatg gtcacgatga ccgcgttctt cagctctttc gagactcgca
    12841 gcgccacgtg catcgcgccg ccggaggaga tgccggcgaa gatgccctcc tcgcgcgcca
    12901 ggcgccgcgt catctcctcg gcttcggcct ggctcacgta ttccagccgg tcgacgcgcc
    12961 gcgcgtcgta gatcttcggc aggtaggcct cgggccactt gcggatgccc ggaatctgcg
    13021 agccctcctg cggctggcag ccgatgacct ggatcgccgc gttcttctcc ttgaagaagc
    13081 gcgagcaccc catgatggtg ccggtggtcc ccatgctcga gacaaagtgc gtaatgcggc
    13141 cgtcggtgtc gcgccagatc tccgggccgg tggtctcgta gtgcaccagc gggttgtcgg
    13201 ggttggcgaa ctggtcgagc atggtgccct tcttctcgcg caccatgcgc tcggcgaggt
    13261 cgcgcgcggc ttccatgccg cccgcctgcg gcgtcaggat gatctcggcg ccgtaggccc
    13321 gcatcgtctg gcgccgctcg gccgaaaggt gctcgggcat gatcagcacc aggcggtagc
    13381 cgcggatcgc cgccgccatg gccagcgcga tcccggtatt gcccgaggtc ggctcgacca
    13441 gcgtgtcgcc gggcttgatg cggccgcgct tctcggcgcg gcggatcatc gagatcgccg
    13501 gccggtcctt caccgagccc gccggattgt cgccttcgag cttggcaagg aggatgttct
    13561 tgtccgaacc gggcaggcgc tgcaggcgga cgagcggcgt attcccgatg aagtcttcga
    13621 cggtcctttt cacctctcct actgctcctg cttcttcagc tcggccatgt acgccgccac
    13681 gccctgctcc acgttcttga actcgcccgg gtagccggcc gcgcgcagcc gcgacaggtc
    13741 ggcctccgtg aagctctggt atttcccgac cagcgccggc ggaaacggca cgtactcgat
    13801 cagccgcttg gcgaccaggt cctgcagcat cgcctgcgtg ccgtgcaccg cgttcaccac
    13861 ggcagcggcg agccggttga aggactgcgc cttgccggtg ccgcaattga acacgccgga
    13921 aacgtcgcgg cgctcgagga accagaggtt caccgccacg acgtcgtcga cgtagatgaa
    13981 gtcgcgccgc tgctcgccgt cgcggtagcc gtcgcagccg acgaacagct tgactcggcc
    14041 ctcggcgtgc agctgctggt acgcgtgcag cgccaccgag gccatgcggc ccttgtgcgc
    14101 ctcgttcggg ccgtagacgt tgaagtagcg caggccgacg acctgcgagg tcctcgtttc
    14161 gagggtgccc ctcacgtact ggtcgaacag gaacttggag tagccgtaga cgttgagcgg
    14221 cttctcgcag cggcgctcct cgcggaattc gggaccggcg ccgtacaccg acgccgacga
    14281 ggcatagatg agcggcactt cctcgtcctg gcaccattcg agcaggcgct tcgagtagcc
    14341 gtagttgttg tccatcatgt agcgcccgtc gctctccatg gtgtcggagc aggcgccctg
    14401 gtggaacacc gcctcgaccg cgccctcgta gcgctcgagg ttcgcgatga actcggcctg
    14461 gtcgacgtag tcggcgatct cgcagccggc gaggttgcgg aacttgtcgg agtgctggag
    14521 gttgtcgacc gcgatcacct cggtgatgcc gcggcggttg aggccctcga ccagcttcga
    14581 gccgatgaag ccggccgccc cggtgacgat ggtgtaagtc acttgctgcc ttttccgaaa
    14641 agctcggcgt gcgtggcggc cgcggtgccg agcttgccca ccacgatgcc ggcggcccgg
    14701 ttggcgatgc gcaccccggc ttccagcgtg gcgccggcgg cgagcatcac cgcaagggtg
    14761 gcgatcacgg tgtccccggc gccggtgacg tcgaacacct cgcgcgcctc ggccttgatg
    14821 ctcatcacct tcttcccgaa cagcgtcatg ccgtccccgc cgcgggtcag gagcaacgct
    14881 tcgagcttga gcttgtcgcg cagcgcctcg gcgcgggcct tgaggtcctt ctcgtccttc
    14941 caggtgccga cgacctcgcg cagctcggcg aggttgggcg taatgatgct cgcgcccttg
    15001 tagcgcgcat agtcgtctcc tttcggatcg accagcacgc gcttgccggc gcggcgcgcg
    15061 ctgcgaatca tttccgcgat gtggtgcagc ccgcccttgc catagtcgga gagaatgatg
    15121 acgtcgctct ccggcagcgc gcgcttgaag tcctccagct tggacgcgag cacctcgcgt
    15181 gagggcgggc gctcgaaatc gatgcgcagc agctgctggc gcctgccgat cacgcgcagc
    15241 ttctgcgtcg tgctcaggct gcggtcgtga tgcaggctcg cttcgactcc cattttcttc
    15301 agcagcttct cgagcgagcg tcccggctcg tcggtgccga cgaccgagag cagccgggtg
    15361 cgcgcgccga gctccttgca gttccacgct acgttggcgg cgccgccgag cttcagcacc
    15421 tcgcttttct cgttcaccag gacgacgggc accggcgcct cgggcgagat gcgcgacacc
    15481 tcgccgaacc agtagcggtc gagcatgacg tcgcccacca ccagcactct cgccttggcg
    15541 gtatctatga ctttctcccg atcgggaaat actcgaagcc gagacccttg aggacgcttg
    15601 ggtcatagag gttacgcccg tcgaagatcg cgggagtctt gaggcgcgcc ttgatcgccg
    15661 cgaaatccgg gctgcgaaac tcccgccact cggtcaccac ggcgagcgcg tcggccccct
    15721 ccagcgccgc gagggcggag tcgacaattt cgatgccggg gctgtcgcgg tagagccgct
    15781 ttccctcgtg gcccgccgcg ggatcgtaag cgcggacgcg cgcccccgcg cctaccaggt
    15841 cggagatcaa ggtgcggctc gacgcctcgc gcatgtcgtc ggtatttgcc ttgaaagcga
    15901 gcccccagac cgcgaagcac ttgccgttca gccggtcgcc gaagcgccgc ttgatcttcg
    15961 ccgcgacgac gtgcttttgc gcctcgttga ccgcttccac ggcagccacc acgcgcaatt
    16021 cccgcttggc ctccgacgcg gtgcgctgca gcgctctcac gtccttcgga aagcaggagc
    16081 caccatagcc cgctcccggg tacaggaagg cggtgccgat gcgcgggtcg gcgccgatgc
    16141 cgtggcggac ctcttcgacg tccgcgccga gggcctcggc gatattcgcg atctcgttca
    16201 tgaacgagat gcgtgtggcg agcatggcgt tcgccgcgta cttggtgagc tcggccgagc
    16261 gcactcccat gacgatgatg cgctcgcggt tgcgctggaa cggcgcgtag agctggcgca
    16321 agagcgcgat ggcgcgcgga tcttcggagc cgacgatgat gcggtcgggc cgcatgaaat
    16381 cctcgatcgc ggcgccttcc ttgaggaact cgggattgga aaccacggag aagggaacgt
    16441 cggtctcttt tttcacggct tccgcgatcc ggtccgcggt gccgaccggc accgtcgact
    16501 tggtggcgat caccttgtag gacttcatgt gtttgccgat ggcgcgcgcc gcggccagca
    16561 cgtactgcat gtcggccgag ccgtcctcgt ccggcggcgt gccgaccgcg atcagctgca
    16621 gctccccgtg cgccacgctc gccgccacgt cggtcgagaa gcgcagccgg ccggcggcct
    16681 cgttgcgctg cacgatcggc tcgagcccgg gctcgtagat cgggatgcgc ccggcgttga
    16741 gcgcgcgaat tttctgctcg tccacgtcga ggcagaacac gttgttgccc gcgtcggcga
    16801 ggcaagcgcc agtaaccaag cccacgtatc ccgttccaat gatcgtgacg ttcatggcgt
    16861 aaggtcgaac tcctcagtgc ggcgcggcgg ataggtttcc cagccgccgc aggccgggca
    16921 atgccagtgg aactggcgcg ccttgaagcc gcagctctcg cagcgataac gggcaagacg
    16981 ccgcgtatga ccatggacga ggttgcgcac caactccagg tcgcggcgct tgtcgggcga
    17041 gcccgcggcg atgatttgcg cttcgagcag gcggtcgagg ccgagcagcg tcgggttgcg
    17101 gcgcagctcg tcgcgcacca tggcgtaggc ctgctcgggg cccttcgctt cgagcgtctg
    17161 ctggaacacg ttgtcgagca ggtcgagcga gggatagcgc tcgaggtagc ccgcgagcag
    17221 cgtcaggccc tcctcggcgc gcccggcgtc gcggtaggcc tcgaccaggc gctgcgcgac
    17281 cagcgcgagg tacgccgggt cctgcgactc gacgcgcatc cagtgctcga cggcgcgatc
    17341 gatgttgttg gcgcttcgtt ccaggtcacc ccacagcagg ctcgcgcgca cgcacttgcg
    17401 gttcgcctcg agcgcggcgt cgaggtggcg gcgggcggca tcgggatgcg actgcgccga
    17461 ttcggaggcg gcgagctcgc acaggtactg ggcgagcgcg cgcggatcgg tctcgacctg
    17521 cttggtcatc tcgatggcgc gcggccagtc cttctcctgc tcgtagatct ccaacagaaa
    17581 gcggcgcgcc gcggtcccct gggtggtttt ctcgagtcgg gcaaagactt cctccgcgcg
    17641 gtcgaggatc ccggccttga ggtagtcctg gccgagctcc atctgcgcct gcagcttctg
    17701 ctcgccggcg aggtcggcgc gctcgagcag gttctggtgc atgcggatgg cgcggtcgta
    17761 ctcgccgcgc cggcggaaca ggctccccag cgcgaagtgc agctcgatcg tttcgggatc
    17821 gaccttgacc acctcgatga acgcctcgat cgccttgtcc ggctgctcgt tcagcaggaa
    17881 gttcaggccc ttgaagtagg agcgcggcag cgcgcgggat tcggacacga tctgcttgat
    17941 gtcgatgcgc gcggcgatcc agcccagccc gaagaatagc gggaacccga gcagccacca
    18001 gtattcgaac tccatggcta gacgagcggg gggatcggcg gcacgaccgg ttccaggggt
    18061 ctcttgagaa gcgagatctg ccggcgctgc cgcagcaccg tagtcagcag ggcgagcacg
    18121 ccgaacagcg cgccgagagc gaaggcgccg agcaggagga gcaccagcgg cgcctgcagc
    18181 gcgagatcga aatagaagcg cagcaccacc ggctcgacat tcttcgctgc gaaggcgatc
    18241 aggagcagga aaacgccgac gcggatcgtc caggtgacga tacgcatgaa gacgccgcga
    18301 gttttccctg tcgacctagc ggaccggttg ggaagcgcgc ggctgcggcg cggacgaaga
    18361 ctgcggcgtc gccgagtcga cgcgctcgcg cagctccttg ccggccttga agtgcggcac
    18421 gtacttctcc ggcacctgca ccttctcgcc ggatttcggg ttacgcccgg tgcgcggcgg
    18481 acggtagttg agcccgaagc tgccgaagcc gcggatctcg atgcgatgcc cgctgagcag
    18541 gctctgcgtc atcgcgtcga ggatcatctt caccgcgtat tcggcgtcct tcgccaccag
    18601 ctgcgggtag cgctgggcaa ggcgcgcaat cagttccgac ttggtcatgg cgaccattcc
    18661 tcccctttgc cggtcttatt gctgcgggtt gccgtctttc ttgtccagct tcgcccgcag
    18721 cagggcgccc agattcgtcg ccccggcgct tgccgccgcc tgctcggtgc gcagctgctt
    18781 catcgcatcg gtctcctcgg cgaggtcctt cgccttcacc gagagattga tcgagcggtt
    18841 cttgcgatcg acgttgatga tcatcgcctg cacgctgtcg ccctccttca ggtgcttggt
    18901 gaggtcgtcg atgcggtcgc gcgcgaactc ggaggcgcgc aggtagccct cgacgccgcc
    18961 gatgtccacc accgcgccct tggcgtcgac cgacttcacc gcgccggtga ccacgctgtt
    19021 cttctcgtgg ccggcgatga agttggtgaa cggatcgccc tcgagctgct tgatgccgag
    19081 cgaaatgcgc tcgcgctcga tgtcgatcga gagcacgacc gcctccacct cctcgccttt
    19141 cttgaagttc ttgaccgcct cctcgccggg cgccgaccac gaaagatcgg acagatgcac
    19201 caggccgtcg atgccgccgg gcaggccgac gaacacgccg aaatcggtga tcgacttgat
    19261 ctggcccttc accttgtcgc tcttcttgta ctcgcgcgcg aagtcttccc acgggttggg
    19321 catgcactgc ttcatgccca gggaaattcg gcggcgctcc tcgtcgatct cgaggatcat
    19381 cacctcgacc tcgtcgccca cctgcaccac cttggacggg tgcacgttct tgttggtcca
    19441 gtccatctcg gagacgtgca ccaggccctc gatgcccgac tcgacctcga cgaacgcgcc
    19501 gtagtcggtg aggttcgtga ccttgccgaa caggcgcgtt ccctgcggat agcgccgcgc
    19561 gatgccgacc caggggtcct cgccgagctg cttgagcccg agcgagacgc ggttcttctc
    19621 ggagtcgaac ttgaggacct tggcggtcac ttcgtcgccc acgttgagga cctcggacgg
    19681 atgcttcacg cgccgccagg cgaggtcggt gatgtgcagc aggccgtcga tcccgccgag
    19741 atccacgaac gcgccgtagt cggtgatgtt cttgaccacg cccttgacgg tggcgccttc
    19801 ctgcagcgag gcgaggagct gctcgcgctc ggcgcccgcg gtctcctcga gcaccgcgcg
    19861 gcgcgatacg accacgttgt tccgcttgcg gtcgagcttg atgaccttga actccatctc
    19921 cttgccttcg tacggcgtgg tgtccttgac cggccgcagg tcgaccagcg acccgggcag
    19981 gaaggcgcgg atgccgttgg ccatgaccgt caggccgccc ttcaccttgc cggtaatgac
    20041 gcccttgatc ttctcgccgc tctccagcgc cttttcgagg ccgatccag
//
LOCUS       DATBSZ010000110        23758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2133187, whole genome shotgun sequence.
ACCESSION   DATBSZ010000110 DATBSZ010000000
VERSION     DATBSZ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23758
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2133187"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="VIG70_07175"
     CDS             complement(<1..116)
                     /locus_tag="VIG70_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEY8250373.1"
                     /translation="MDRLILEFDRALRAIAGVAHASRPSPAERLPEQPVSEEG"
     gene            complement(188..1423)
                     /locus_tag="VIG70_07180"
     CDS             complement(188..1423)
                     /locus_tag="VIG70_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY8250374.1"
                     /translation="MQKKLLGLAVAGALAAPGAALAQVEVYGFISVAVSNFKYSDGTA
                     AGTPSVSKWDVSSHGTNYGVRGRENLGGGLTAWFQIEQNAPMERSNNVAITPASRNSA
                     VGFQGNWGNFFIGQWTTPWADLESLWSIGAVGGYSPSLSIIGRRETTGTSPNPNCING
                     HVSGAVGFTTTQPVCDPVEAAGGIGHPFWRRISQSIFYYSPVFGGAQVKLAYQTNDGK
                     STQSNATTTVADPSMWSAAVQWSGMGGRARVGAAFDSHKDFSTIGQTDSGFAIKGGYD
                     FGIVDVGLAYESMTYKSATGDCDAVQMGVALAIPIGQGHIRASYSQADDLDGTFNGAG
                     TCGAAGAGDNGAKEYTIGYDYRFSKRTTVGVGYAQIKNDPSAVFTWTGAPGNQSGTGL
                     APFPGSDPSAFFLSVIHRF"
     gene            complement(1609..2370)
                     /gene="trpC"
                     /locus_tag="VIG70_07185"
     CDS             complement(1609..2370)
                     /gene="trpC"
                     /locus_tag="VIG70_07185"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY8250375.1"
                     /translation="MSILERIVAAKRTEVAAAKTRVPEVKLDAAPAVRDFIGALRAKR
                     PAVIAEIKRASPSKGLLRSNFDPAAIAKSYEKGGAACMSVLTDREFFQGAAEHLSTAR
                     GACALPVLRKDFLIDPYQVFESRALGADCVLLIAACLEDRQMRELEALARSVGMAVLV
                     EVHDGEELERALKLDTPLLGINNRNLRTFETRLETTLELLPRVPHDRLVITESGILSR
                     ADVARMRQRGVEAFLVGEAFMRAPDPGAELKALFA"
     gene            complement(2367..3407)
                     /gene="trpD"
                     /locus_tag="VIG70_07190"
     CDS             complement(2367..3407)
                     /gene="trpD"
                     /locus_tag="VIG70_07190"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY8250376.1"
                     /translation="MKQITIAEAIQRTVEHREVFHDEMLHVMRQIMRGELSPAQIAGF
                     IVGLRVKKETIGEIAAAAQVMRELATPVEVKDDRHLVDTCGTGGDAAHTFNISTCAAF
                     VAAAAGAKVAKHMGRSVSSSSGSAEVLEALGAQIMLTPADSAESIDKLGIGFMFAPAH
                     HAAMKHAAPVRKDLGVRTIFNILGPLTNPAGAKNQVMGVFHPDLVGIEVRVLQRLGSK
                     HVMVVHGLDGLDEISVSGPTMVGELANGEISEYLVHPAELGMELYDRHAIEVHTVAES
                     KAMIQAVLENQPGPAHNIVALNAGAAIYVSGVAKSFKAGIERAVQAIKSGAAKQKLEE
                     FVAFTQKVKVKA"
     gene            complement(3404..3976)
                     /locus_tag="VIG70_07195"
     CDS             complement(3404..3976)
                     /locus_tag="VIG70_07195"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY8250377.1"
                     /translation="MLLMIDNYDSFTFNLVQYFGELGAEVKVFRNDELDVQKIEALAP
                     SHIVISPGPCTPNEAGVSLETIERLAGRIPILGVCLGHQAIGQAFGGKVVRAKHVMHG
                     KVSRIHHDGRGVFSKLDNDFVATRYHSLVVERASLPACLEISAQSEDGEIMGLRHREA
                     PVEGVQFHPEALLTEHGHKLLENFIKGEKS"
     gene            4149..5669
                     /locus_tag="VIG70_07200"
     CDS             4149..5669
                     /locus_tag="VIG70_07200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8250378.1"
                     /translation="MESEIAFAHAQLSARQQAEGAYGPALESMTTALRLAPGAAALWA
                     QFSDLIRYFNLRHPVPPAVRELLSKALEHPAVDPGNLVRPITTLALSRPAGTAFEEPL
                     LLRMLEDVVIRDGELERLITDTRRRMLDEHVPANIALAIAHQCFNTEYVFDETAEERA
                     KVDRLSVDSPHAYAAYAAYRSLGTLKDVKLDGTSLARRQIVEPMEERRLAAEIASLST
                     EKGSVSAAVRAQYEANPYPRWVRMQSVFAAAPLAQVVHELFPHARARFISQTPRILVA
                     GCGTGQNAIATAQRFSGASVLAVDLSLASLGYARRKTLELGLKNLQYLQADILALGAL
                     EERFDLIECSGVLHHLEDPFEGWRILAALRKPGGLMRVGLYSEAGRRPIARARELIAA
                     RGFVPDAEGIRTCRAAIRADPSFVQVARNEDFYSMSGCRDLLFHVHEHHFNLPQIEAM
                     IARLGLRFLGFEFPDSGATLGRFRAKFGQDSSLENWHRFEQEFPDTFARMYQFWVS"
     gene            complement(5651..7117)
                     /gene="trpE"
                     /locus_tag="VIG70_07205"
     CDS             complement(5651..7117)
                     /gene="trpE"
                     /locus_tag="VIG70_07205"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEY8250379.1"
                     /translation="MSYTRIPVVAEARADLYTPLAVYLKLANGPYSYLLESVVGGERF
                     GRYSFIGLACAERIEARTTARGTTVRRLLRDARGADVCLESVEADPFEYARAWLAGHR
                     VAPVPAALRFGGGLAGYFGYESVRNIEKNALKEEKPDALGTPDMLLLVSDELAVVDNV
                     LGKLYLVVYADPRQPDAERNAKARLEHLRSRLAGPLPDERFFENQKPKEAKLDCSFTE
                     AGFLDAVRRAKEYIFAGDVMQVQISQRTSRAFDAPPLALYRALRGVNPSPYMYYFDFG
                     DHHVVGASPEILVRLAGETVTLRPIADTRPRGTNPQEDAATAEELLADPKERAEHVML
                     IDLGRNDVGRIAATGSVKVTERMVIERYSHVMHIVSNVEGRLAAGLGALDVLKASFPA
                     GTVTGAPKVRAMQIIDELEPVKRGVYSGAVGYLGFNGDMDVAIAIRTAVVKDRRLHVQ
                     AAAGVVADSDPRSEWLETQHKARALLRAAEIADQDTQN"
     gene            complement(7114..7770)
                     /gene="rpe"
                     /locus_tag="VIG70_07210"
     CDS             complement(7114..7770)
                     /gene="rpe"
                     /locus_tag="VIG70_07210"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY8250380.1"
                     /translation="MTFRIAPSILSADFSRLGDEVRAVERAGADLIHFDVMDNHYVPN
                     LTVGPLVCAALKPHVKLPLDVHLMVKPVDALVPEFAKAGASIISFHPEASDHVDRTIA
                     LIKEHGCRAGLVLNPATPVSWLDHTLEKLDLVLLMSVNPGFGGQQFIRSVLPKITEVR
                     RRIDALKKQIWLEVDGGVKIDNIGEIARAGADTFVAGSAIFGSKDYAATIREMRTRLS
                     "
     gene            7797..8189
                     /gene="apaG"
                     /locus_tag="VIG70_07215"
     CDS             7797..8189
                     /gene="apaG"
                     /locus_tag="VIG70_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY8250381.1"
                     /translation="MAQEKQEKLYEVAVNAATQYLADQSDESAGRYVFAYTITIKNTG
                     AVAAQLISRHWVITDAQGLVQEVRGLGVVGAQPLLQPGERFEYTSGASIATPVGTMRG
                     SYQMVATDGTRFEAAIPEFTLSVPRILH"
     gene            complement(8186..8587)
                     /locus_tag="VIG70_07220"
     CDS             complement(8186..8587)
                     /locus_tag="VIG70_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250382.1"
                     /translation="MSPLSPLSLDTTAYGTNSVSKNLRAASSSADGRLFARSQTLLNR
                     AQPAMNARKLGSSCARLQPGRSAKPRASYRAVSGCGFGGGQTGHFTGAGAHAATIRIK
                     TAQRMWIIYLEIIVVLALVALIVWFTWPKKP"
     gene            8556..9401
                     /locus_tag="VIG70_07225"
     CDS             8556..9401
                     /locus_tag="VIG70_07225"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEY8250383.1"
                     /translation="MSSESGDSGLITGYYEPIIDGSRTPSAAHRYPIYGVPADLVVVD
                     LGAVSAETRPLRLRGRLDGRRLVPYYSRGEIDARGDFAAPVIAWSGDPVELFFLQVQG
                     SGQVRLENGERIRVGYADQNGHPYRSLGRHLVDRGELVLEQASMQGIKAWAAVNPQKL
                     QEALNQNPSYVFFRELPPTDGPIGALNIPLHAERSLAVDRRFVPLGAPVYLATTFPLS
                     EEPLERLMAAHDTGGAIRGVVRADFYWGTGPEAGAQAGRMRQQGKMWLLWPTGEPLPA
                     GPAAP"
     gene            complement(9361..10092)
                     /locus_tag="VIG70_07230"
     CDS             complement(9361..10092)
                     /locus_tag="VIG70_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEY8250384.1"
                     /translation="MIARFFILITVFFVNAALANEAQIRKALEPKLSGAKIEGIQPGP
                     VPGLFEVRFRTSEGGVQIVYTDASATHVFVGRVYEVRTDRNLTEERTRALNAIKFESL
                     PLDLAVKVQRGNGKRVLAMFSDPYCPACRQFERALAQIDDISVYVFMYPVIRPENSDH
                     SRAVWCSPDRAKAWLELAAAAQPKVPQAGTSCPNPLDKVHELGRKLGVNSTPTLFLAN
                     GERLSGGLGPDDLKELLDRPATARR"
     gene            complement(10089..11237)
                     /locus_tag="VIG70_07235"
     CDS             complement(10089..11237)
                     /locus_tag="VIG70_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HEY8250385.1"
                     /translation="MINPGRHDLAVVGGGPVGASLARAARGMSVLLVAQQRPAPIGSG
                     FDARVYALSPGNVAFLKAIGAWQAIPEDRLVPVHAMRIHGDDASRLDFDAYSAGVPQL
                     AWIVEDALLQDALWRDLEADVLVAQCGGLSIDEDWATLGMHDGGRIGAKLIVGADGAH
                     SFVRRQAGIGATDRDYGQSAVVANFACGKPHGNVAYQWFQRGAVLALLPLPGNHVSMV
                     WSLPTEQARSTGALEGEALCRAVEVAAGGVLGELETVTAPRSYPLRRLAAERLVAPRV
                     ALAGDAGHVVHPLAGQGLNLGLQDARTLAAVLHSREPQRDPGELRLLRRYERGRAEPI
                     LAMDTMVDSLFRLFGAEDRFAARLRNAGLNLTDRLPVVKSMLMRQAMT"
     gene            11253..11329
                     /locus_tag="VIG70_07240"
     tRNA            11253..11329
                     /locus_tag="VIG70_07240"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11287..11289,aa:Met,seq:cat)
     gene            complement(11335..11700)
                     /locus_tag="VIG70_07245"
     CDS             complement(11335..11700)
                     /locus_tag="VIG70_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8250386.1"
                     /translation="MDVLIIDDHPIIHETLTAMVRKAIPGASVHAESELGAAIAIGMG
                     LADFRLALLDLGLPGYSGIEALLRFRSAFPKVRVIVVSATEDPVTMERAIAAGAAWYL
                     PKTAKPYAMVAAIKSTAGV"
     gene            complement(11703..12455)
                     /gene="tatC"
                     /locus_tag="VIG70_07250"
     CDS             complement(11703..12455)
                     /gene="tatC"
                     /locus_tag="VIG70_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY8250387.1"
                     /translation="MSDQESFISHLIELRARLVRAVGAVLAIFVVLFLYPGSGYIYDL
                     LALPLMNALPEGSKMIATGVITPFMVPVKVTALMGFMVALPYVLYQAWAFVAPGLYEH
                     EKKLAMPLIVASTVLFFLGVAFCYFFVFGKVFAFIHDFAPKSITPAPDIEAYFSFVIT
                     MFLAFGITFEIPIVVIVLVRMGVVTVEKLREARPYVIVGAFVVAAVVTPPDVLSQFML
                     AVPMCLLYEAGVFLARFVGTRPKAESEYKTQP"
     gene            complement(12605..12832)
                     /gene="tatB"
                     /locus_tag="VIG70_07255"
                     /pseudo
     CDS             complement(12605..12832)
                     /gene="tatB"
                     /locus_tag="VIG70_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014906112.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
     gene            complement(12909..13133)
                     /gene="tatA"
                     /locus_tag="VIG70_07260"
     CDS             complement(12909..13133)
                     /gene="tatA"
                     /locus_tag="VIG70_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEY8250388.1"
                     /translation="MGSLSIWHWLIVLVIVMLIFGTKKLRNMGTDLGGAVRGFKDGMR
                     EGGEKPADPAPQVSGKTIEGEVKGKTEQKA"
     gene            complement(13143..13502)
                     /locus_tag="VIG70_07265"
     CDS             complement(13143..13502)
                     /locus_tag="VIG70_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEY8250389.1"
                     /translation="MNDPNCIFCKIVRGEIPAKKIYEDAEVLAFPDIRPQAPVHLLVI
                     PKVHIATLYDAGAAHHAALGKMLAVAGRLAREAGAADGFRTIVNTGRVGHQEVYHVHM
                     HVLGGPDPLGPMLQRQR"
     gene            complement(13510..13839)
                     /locus_tag="VIG70_07270"
     CDS             complement(13510..13839)
                     /locus_tag="VIG70_07270"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEY8250390.1"
                     /translation="MKLGQAETLERIAASINERKGGDPAKSYVAKLFAQGDDAMLKKI
                     GEEATETLLAAKSGDRLHLVREVTDLWFHCMIVLARHGLGPGDVIAEMQRREGISGID
                     EKARRDK"
     gene            complement(13836..14219)
                     /gene="hisI"
                     /locus_tag="VIG70_07275"
     CDS             complement(13836..14219)
                     /gene="hisI"
                     /locus_tag="VIG70_07275"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY8250391.1"
                     /translation="MNFLDEVPWNADGLVAVVAQDAASGRVLTVAWMNRDALKETAET
                     KRAVYWSRSRKRLWRKGEESGHVQKVLEVRIDCDADAILLKVEQVGGIACHTGRESCF
                     FRKLENGRWVTIDPVLKDPSLIYKK"
     gene            complement(14216..14974)
                     /gene="hisF"
                     /locus_tag="VIG70_07280"
     CDS             complement(14216..14974)
                     /gene="hisF"
                     /locus_tag="VIG70_07280"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012362933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY8250392.1"
                     /translation="MPLAKRVIPCLDVTAGRVVKGVNFVGLRDAGDPVEIAARYDGEG
                     ADEVCFLDITASSDERDMLLHVIEAVAERVFIPLTVGGGVRQVGDVRRLLNAGADKVS
                     INTAAVQNPQLVRDAAGIVGCQCIVVAIDAKKNSTGWEVFTHGGRKATGLDAVEWARR
                     MAHAGAGEILLTSMDRDGTRDGFDLGLTRAVADAVGVPVIASGGVGSMEHLAEGVLAG
                     GADAVLAASVFHFGDFTVRAAKEHMRARGIEVRL"
     gene            complement(14977..15726)
                     /gene="hisA"
                     /locus_tag="VIG70_07285"
     CDS             complement(14977..15726)
                     /gene="hisA"
                     /locus_tag="VIG70_07285"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEY8250393.1"
                     /translation="MLIIPAIDLKDGRCVRLKQGDMSTATVFSEDPVAMARHWAAEGA
                     KRLHIVDLNGAIAGRPKNEKVIRDIVAAVGDAVPIQLGGGIRDLDAIESYIDAGVTYV
                     VIGTAAVKNPGFLSDACYAFPGHVIAGLDAKDGKVAVEGWSKLTGHDVIDLAKKFEEY
                     GVEAIIYTDIGRDGMMSGVNIDATFKLAQQLKTPIIASGGLASLKDVQAVCEKLVPEG
                     IIGVIAGRALYEGRIDLKKAQQAANKALGKE"
     gene            complement(15746..16357)
                     /gene="hisH"
                     /locus_tag="VIG70_07290"
     CDS             complement(15746..16357)
                     /gene="hisH"
                     /locus_tag="VIG70_07290"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY8250394.1"
                     /translation="MRIAVVDYGMGNLRSVSKALEHVAPQAEVLVTAEPGAISSSERV
                     VVPGQGALPDCMRQLEASGAREAVVEAAARKPFLGICIGLQMLFERGEEGDAAGLGLL
                     PGRVPRFALTGLKIPHIGWNEVAQTRPHDLWAGIPDLSRFYFVHSYYPAPRDAALTAA
                     TAVYGAPFTCAIARDNIFAVQFHPEKSQSAGLRLLSNFVRWRP"
     gene            complement(16365..16958)
                     /gene="hisB"
                     /locus_tag="VIG70_07295"
     CDS             complement(16365..16958)
                     /gene="hisB"
                     /locus_tag="VIG70_07295"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY8250395.1"
                     /translation="MRSAEVTRNTKETQIRVKLNLDGKGAAKLSTGLPFLEHMLDQVA
                     RHAMLDLDIEAKGDLHIDAHHTVEDIGITLGQAVAKAIGDKAGVRRFGHAYVPLDEAL
                     SRVVLDLSGRPGLEYHVKFTRALIGEFDVDLMHEFFQGFVNHAQVTLHIDNLRGDNAH
                     HQCETMFKAFARALRMAAEPDPRAAGAVPSTKGTLQG"
     gene            complement(16955..18013)
                     /gene="hisC"
                     /locus_tag="VIG70_07300"
     CDS             complement(16955..18013)
                     /gene="hisC"
                     /locus_tag="VIG70_07300"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY8250396.1"
                     /translation="MKPEDLVRPEILALKAYPVPSSEGMVKLDAMENPYTLPDGLRRE
                     LAAVLARVDVNRYPTPSPQELREAIARRMSVPAGMEVLLGNGSDELIQMLITALARPG
                     AAMMFPSPTFVMYSMGATFSGMRAVPVPLREDFSLDADAFIARMQAEKPALVFLAYPN
                     NPTGVLYPEADVVKIIRASSGLVVVDEAYHVFARKSFLPRLAEFGNLVVIRTVSKLGL
                     AGIRLGYLVGRPEWVAQLDKVRPPYNVSALTQAAATFMLERLEVLEEQAARIRSERKN
                     LGEALQGLKGVTVFPSEANFFLVRVPDADRAFEALKRQGVLVRNLNSPALKNCLRVTV
                     GTPDENRILLNALREALQ"
     gene            complement(18010..19272)
                     /gene="hisD"
                     /locus_tag="VIG70_07305"
     CDS             complement(18010..19272)
                     /gene="hisD"
                     /locus_tag="VIG70_07305"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY8250397.1"
                     /translation="MKLRRLSTQQRNFSEKLSALTRYEATQDPAVQKAVRRILADVRK
                     RGDAAVRDYTKKFDGVAPKQYAVSEKFLEAIPAEQAQALRAAHDRIRAFHEKQLQKSW
                     EFTDADGTRLGQRLSPLERVGLYVPGGKAAYPSSVLMNAVPAKVAGVRELVMASPNPN
                     ALVLAAAALAGVDRVLGIGGAQAIAALAYGTKSIPRVDKIVGPGNAYVAEAKRQVFGE
                     VGIDMLAGPSEILVIADGAAPADWVAMDLFSQAEHDESAQAILLSPNEGYLDEVAASI
                     RKLLDEMPRRKVIAASLKARGALILTRDLEEACAVANRIAPEHLELSVENPEGLVEKV
                     RNAGAIFLGRWSSEAIGDYCAGPNHVLPTAGTARFSSPLGVYDFQKRTSVIRVSQSAA
                     AKLGRVAMTLAEGEGLTAHARAAEMRFK"
     gene            complement(19269..19910)
                     /gene="hisG"
                     /locus_tag="VIG70_07310"
     CDS             complement(19269..19910)
                     /gene="hisG"
                     /locus_tag="VIG70_07310"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012327780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY8250398.1"
                     /translation="MASISIALSKGRIFDETAPLLARVGIRPKENPETSRKLVIGTNR
                     RDLRLVVVRAADTPTYVQHGGADLGIAGRDVLAEHGGEGLYQPVDLRIAACRMMVAVR
                     KGFDYAAAVRQGARLRVATKYLKTAREHFAAKGVHIDLIRLYGSMELAPLVGLADAIV
                     DLVSTGKTLKANGLVAVEEIMPVSARLIVNQAALKTKRATLQPLIEAFAEAAR"
     gene            complement(19971..21224)
                     /gene="murA"
                     /locus_tag="VIG70_07315"
     CDS             complement(19971..21224)
                     /gene="murA"
                     /locus_tag="VIG70_07315"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY8250399.1"
                     /translation="MDKLKITGGRPLEGEVRVSGAKNAALPIMCAALLTAKPLNISNV
                     PHLRDVLTMAKLLREMGLRVESSPEGQVALHAAAIKEPTASYELVKTMRASVLVLGPL
                     LARCGRAKVSLPGGCAIGARPVDQHVKGLEAMGATIAVEHGYMHASAERLRGARIVMD
                     LVTVTGTENLMMAASLAEGETLLENAAREPEVVDLARCLTAMGARIDGAGSDTIRIQG
                     VRSLGGAEYRVMPDRIETGTYLAAAAATGGKVRLTGTAPDSLDATLDKLREAGAKIRI
                     QGSSIELEADRKSDAVSIRTAPYPGFATDMQAQFMALATVARGTALITETIFENRFMH
                     ALEMQRLGADIRLEGNTAVVKGVERLQGAKVMATDLRASAGLVIAGLVADGDTVVDRI
                     YHLDRGYEALETKLSSLGARVVRIR"
     gene            complement(21256..21501)
                     /locus_tag="VIG70_07320"
     CDS             complement(21256..21501)
                     /locus_tag="VIG70_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY8250400.1"
                     /translation="MVTPESVKSGIEAGLACDHVEVVGDGQHFQALVVSAQFAGRNRV
                     QRHQLVYKALGDRMREEIHALSMQTLTPDEWKKSGTP"
     gene            complement(21505..22257)
                     /locus_tag="VIG70_07325"
     CDS             complement(21505..22257)
                     /locus_tag="VIG70_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8250401.1"
                     /translation="MSFWTLFAKELLRFVKVSVQTVAAPVLTSLLYLVIFGQALEGRV
                     QVFDGVRYTAFLVPGLVMMSVLQNAFANSSSSLIQSKITGNIVFILLAPISYAEFFWA
                     YVAAAVARGLAVGAGVLAVTAWFVDLRLEAPLWTAAFALLGAALLGALGLLAGIVSEK
                     IDQLAAFQNFVILPLTMLSGVFYSIRSLPAFWRGLSHANPFFYMIDGFRYGFFASSDF
                     PPGVSLAVVAVAFGAVAALCLLLLKSGYRLRT"
     gene            complement(22254..23108)
                     /locus_tag="VIG70_07330"
     CDS             complement(22254..23108)
                     /locus_tag="VIG70_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8250402.1"
                     /translation="MNAIEVRGVEKRYGAVHALSGVSLAIEQGEFFGLLGPNGAGKTT
                     LINVIAGLARADAGSVSVMGADAIADYRRARRMLGVVPQELVFDPFFTVRETLRIQSG
                     YFGIRGNEAWIDEVMHHLDLSAKADANMRSLSGGMKRRVLVAQALVHKPPVIVLDEPT
                     AGVDVELRQGLWQFITRLNRDGHTIVLTTHYLEEAELHCSRIAMLKAGRIVALDSTRN
                     LLASFAGSRSTVRVVARDGRQFTLELQGFGQLEAELARLREQGVEIADLELERPDLEQ
                     VFLQLTRS"
     gene            complement(23105..23389)
                     /locus_tag="VIG70_07335"
     CDS             complement(23105..23389)
                     /locus_tag="VIG70_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY8250403.1"
                     /translation="MIRREGRRLTVSGPVTLATAAQLLEAGRQHLAEGVRTVDLGEVT
                     EMDSALLALALAWLRDARAAKRELAFVNPPEALQTLSRLYGVEELLPLKT"
     gene            complement(23386..>23758)
                     /locus_tag="VIG70_07340"
     CDS             complement(23386..>23758)
                     /locus_tag="VIG70_07340"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8250404.1"
                     /translation="STPEQKKRLTEEFKTLLVRTYASALAAYSEQKFDFRPLRAKPTD
                     TDVTVNVRVLQPGAQAVTLDYSMEKTANGWKCYDVMVGGVSLVANYRTEFANTVRNSG
                     IDGLIKDLQTKNRSLAKQDKK"
BASE COUNT         3878 a         8039 c         7814 g         4027 t
ORIGIN      
        1 ccctcctcgc tgacgggctg ttccgggagg cgctccgccg gcgagggccg gctcgcgtgc
       61 gccacgccgg cgattgcacg caacgcgcgg tcgaattcga ggatcaagcg gtccactgca
      121 aaagactagc agaaagcaga aaaaaaacgg gcacccgaag gcgcccgtct tgtcgatact
      181 gctgcggcta gaaccggtga atcacgctca ggaagaacgc gctcggatcc gagccgggga
      241 aaggcgcgag gccagtgccc gactggttgc cgggcgcgcc cgtccacgta aacaccgccg
      301 aggggtcgtt tttgatttgc gcgtagccaa caccgaccgt ggtgcgcttc gagaaccgat
      361 agtcgtaccc gatcgtgtac tccttggcgc cgttgtcgcc cgcgcccgcc gcaccgcagg
      421 ttccggcccc gttgaaggtg ccgtcgagat cgtccgcctg ggaataggag gcacgaatgt
      481 gaccttggcc gatcggaatg gcaagagcga cgcccatctg cacggcgtcg caatcgcccg
      541 tcgcgctctt gtaggtcatc gattcgtacg caaggccgac gtccacgatg ccaaagtcat
      601 atcccccctt gatggcaaag cccgaatcgg tctggccgat ggtggagaag tctttgtgcg
      661 agtcgaaggc cgcgccgact cgcgcgcgtc cgcccatccc gctccactgc accgctgccg
      721 accacatcga gggatcggcc accgtggtcg tggcgttgct ttgcgtcgac ttgccgtcgt
      781 tggtctgata ggcgagcttg acctgcgcac cgccgaatac cggcgagtag tagaagatcg
      841 actgcgaaat gcgccgccag aacgggtggc cgatgccgcc ggcagcttcg accggatcgc
      901 aaacgggctg cgtggtcgtg aatccgacag cgccggaaac atggccgtta atgcagttcg
      961 ggtttggcga ggtacccgtg gtctcgcggc ggccgatgat cgaaagcgac gggctgtagc
     1021 cgcccaccgc accgatcgac cacagtgatt ccaggtccgc ccagggcgtg gtccactggc
     1081 cgataaagaa gttgccccag ttgccctgga agccgaccgc gctgttccgc gacgccggcg
     1141 tgatggcgac gttgttcgac cgctccatcg gcgcgttctg ctcgatctgg aaccacgccg
     1201 taaggccgcc gccgaggttt tccctgccgc gaacgccata gttggtgccg tggctcgaca
     1261 cgtcccactt cgataccgac ggcgttcccg cagcggtgcc atccgaatac ttgaaattgc
     1321 tgaccgccac cgagatgaag ccgtaaacct caacctgcgc gagtgcagcg cccggcgcag
     1381 ccaatgcgcc cgcgaccgcc aagcccaaaa gtttcttttg catgcgttct cctctccttg
     1441 tagaacgaaa ctggcgcagc gccgactggc ccccgccaac ccctcctttg agaagtcctt
     1501 ggaagtgtgc cgatgcccct acgcgccaac aaggaatagc ccttgttaca gaggagaaac
     1561 ggtggcgatg ttgcgattac ccaacaggct ttgctgcggc gcagcaggct acgcgaagag
     1621 tgctttcagc tccgcaccag gatccggcgc gcgcatgaac gcttcgccga cgagaaacgc
     1681 ctcgacgccg cgttgccgca tgcgggcgac gtcggctctg gacaggatgc cgctctcggt
     1741 gatcaccagg cggtcgtgcg gcacgcgcgg caggagttcg agcgtggtct cgagccgcgt
     1801 ctcgaaggtg cgcaggttgc ggttgttgat gccgaggagc ggcgtgtcga gcttgagcgc
     1861 gcgctcgagc tcctcaccgt catgcacctc gaccagcacg gccataccaa cgctgcgggc
     1921 gagcgcctca agttcgcgca tttggcggtc ttcaaggcag gcggcgatca gcagtacgca
     1981 atcggcgccg agcgcgcgcg actcgaacac ctggtacggg tcgatcagga aatccttgcg
     2041 cagcaccggc aacgcgcacg cccctcttgc tgtagagagg tgttcggcag cgccctgaaa
     2101 gaactccctg tcagtaagca ctgacatgca agccgctccg cctttttcgt agctcttggc
     2161 gatcgccgcg gggtcgaagt tcgatctgag caaccctttt gaagggctgg cacgcttgat
     2221 ctcggcgatc accgccggcc gcttcgcccg caaggcccca atgaaatcgc gcactgctgg
     2281 agcggcatcg agcttcacct cgggtaccct cgtcttggcc gcggcgacct cggtgcgctt
     2341 ggccgcaacg atgcgctcga ggatgctcaa gccttgactt tcaccttttg cgtgaacgcc
     2401 acgaactctt cgagcttctg cttggcagcg ccgctcttga tcgcctgcac cgcgcgctcg
     2461 attcccgcct tgaagctctt ggccacgccg gaaacgtaga tcgccgcgcc ggcattgagc
     2521 gcgacgatgt tgtgcgccgg gccgggctgg ttctccagca ccgcctggat catcgccttc
     2581 gactcggcga ccgtgtgcac ctcgatcgcg tggcggtcgt agagctccat gccgagctcg
     2641 gccgggtgga cgaggtactc gctgatctcg ccgttcgcga gctcgccgac catcgtcggg
     2701 cctgagaccg agatctcgtc caggccatcg agcccgtgga ccaccatcac gtgcttcgag
     2761 cccaggcgct gcagcacgcg cacctcgatg cccaccaagt cgggatggaa cacccccatc
     2821 acctggttct tcgctcctgc gggattggtc agcgggccca ggatgttgaa gatggtgcgc
     2881 acgccgaggt ccttgcgcac cggcgcggcg tgcttcatcg cggcgtggtg cgcgggcgcg
     2941 aacatgaagc cgatgccgag cttgtcgatc gactcggcgc tatcggccgg cgtgagcatg
     3001 atctgcgcgc cgagcgcctc gagcacctcg gcgctgcccg aggacgacga caccgagcgt
     3061 cccatgtgct tcgcgacctt cgcgccggcc gccgcggcga cgaaagccgc gcacgtcgag
     3121 atgttgaacg tgtgcgcggc atcgccgccg gtgccgcagg tgtcgaccag gtgccgatcg
     3181 tccttcacct cgaccggagt cgccagctcg cgcatgacct gcgccgcggc ggcgatctcg
     3241 ccgatggtct ccttcttcac gcgcaggccg acgatgaagc cggcgatctg cgccgggctg
     3301 agctcgccgc gcatgatctg gcgcatgacg tgaagcatct cgtcgtggaa cacctcgcgg
     3361 tgctcgacgg tgcgctggat cgcctcggcg atcgtgatct gcttcatgac ttttcccctt
     3421 tgatgaaatt ctccagcaat ttgtgtccgt gctcggtcag cagcgcttcc ggatggaact
     3481 gcacgccctc gaccggagcc tctcggtgcc gcagccccat gatctcgccg tcctcggatt
     3541 gcgcgctgat ctccaggcac gccggaaggc tcgcgcgctc gacgaccaga gagtggtagc
     3601 gcgtggcaac gaagtcgttg tcgagcttcg agaaaacccc gcgcccgtcg tgatggatcc
     3661 gggaaacctt gccgtgcatg acgtgcttcg cccgcaccac tttcccgccg aacgcctggc
     3721 cgatcgcctg gtgcccgagg cacacgccga ggatcgggat gcggccggca aggcgctcga
     3781 tggtttcgag ggagacacca gcttcgttcg gcgtgcacgg gccgggcgag atgacgatgt
     3841 gcgagggcgc gagtgcctcg atcttctgca cgtccagctc gtcgttgcgg aacaccttca
     3901 cctcggcgcc gagctcgccg aagtactgca ccaggttgaa ggtaaatgaa tcgtagttgt
     3961 cgatcatcaa tagcattgcg gccgctatcc gtttgattct actggttgtg tttgcctgct
     4021 tctgttcgcc gggtttgcct ccggcgcagg cgtggggtat ctaggcgcgc ggcgagcgcc
     4081 agcgccagga caggacgaaa aacggttgca gcttcacgcg gacgattcta gcccagatac
     4141 ttcgggccat ggaaagcgaa atcgccttcg cgcacgcgca gctctcggca cgccagcagg
     4201 ccgagggcgc gtacgggccg gcgctcgaga gcatgaccac ggcactgcgg ctcgcgcccg
     4261 gcgccgcggc gctgtgggcg cagttttcgg atctgatccg ctacttcaac ctgcgccatc
     4321 ccgtgccgcc ggcggtgcgc gagctgctgt cgaaggcgct ggagcacccg gcggtcgatc
     4381 cgggaaacct cgtacgcccg atcaccacgc tggcgctgtc gcggcccgcg gggacggcgt
     4441 tcgaggagcc gctgctgctg cgcatgctcg aggacgtcgt gatccgcgac ggcgagctcg
     4501 agcggctgat taccgacact cggcgccgca tgctcgatga acacgttccg gccaacatcg
     4561 cgctggcgat cgcgcaccag tgcttcaaca ccgagtacgt cttcgacgaa accgcggagg
     4621 aacgcgccaa ggtcgatcgc ctgtccgtcg attcaccgca tgcgtacgcc gcttacgccg
     4681 cctatcgctc gctcggtacc ctgaaggacg tcaagctcga cggcacctcg ctcgcccgca
     4741 ggcagatcgt cgagccgatg gaggagcgac gcctcgcggc ggaaatcgcc tcgctcagca
     4801 ccgagaaagg cagcgtgtcg gctgcggttc gggcgcagta cgaagcgaat ccttacccgc
     4861 gctgggtgcg catgcaatcg gtcttcgccg ccgcgccgct cgcgcaggtg gtgcatgagc
     4921 tatttccgca tgcccgtgcc cgcttcatct ctcagacgcc gagaattctg gttgccggct
     4981 gcggcaccgg gcagaacgcg atcgccacgg cgcagcgctt ctccggggcg agcgtgctcg
     5041 cggtggatct cagcctcgca agcctgggct atgcgcgacg caagacgctc gagctcggcc
     5101 tgaagaacct ccagtacctg caggccgaca tcctcgcgct cggcgccctg gaagagcgct
     5161 tcgacctcat cgagtgctcg ggggtgctgc accacctcga ggatccgttc gagggctggc
     5221 ggattctcgc ggccttgcgc aagcccggcg gcttgatgcg ggtgggcctt tacagcgaag
     5281 ccggccgccg ccccatcgca cgcgcgagag agttgatcgc tgctcgcggc ttcgtacccg
     5341 atgccgaggg catccgcacc tgccgggcag caattcgtgc ggatccctca ttcgtccagg
     5401 tcgcgcggaa tgaggacttc tacagcatga gcggctgccg cgacctcctg ttccacgtgc
     5461 acgagcacca cttcaacttg ccgcagatcg aagcgatgat cgcccggctc ggtcttcgct
     5521 tcctgggctt cgagtttccc gacagtggcg ccaccctcgg ccgtttccgg gcgaagtttg
     5581 gccaagattc aagtctcgaa aactggcacc ggttcgagca ggagtttccc gacaccttcg
     5641 cccgcatgta tcaattctgg gtgtcctgat cggcaatctc ggcagcccgt agcaacgcgc
     5701 gggccttgtg ctgcgtctcg agccattccg agcgcgggtc cgaatccgcc accacgcctg
     5761 ctgcggcctg cacgtgcagc cgccggtcct tgaccaccgc ggtgcggatc gcgatcgcca
     5821 cgtccatgtc gccgttgaag ccgaggtagc cgaccgcgcc gctgtagacg ccgcgtttca
     5881 ccggctcgag ctcgtcgatg atctgcatcg cgcgcacttt cggcgcgccg gtcactgtgc
     5941 cggccgggaa gctcgccttc agcacgtcga gcgcgccgag acccgccgcg aggcggcctt
     6001 cgacgttcga cacgatgtgc atcacgtgcg aatagcgctc gatcaccatc cgctcggtaa
     6061 ccttgacgct gccggtggcg gcgatgcggc cgacgtcgtt gcgcccaaga tcgatcagca
     6121 tcacgtgctc tgcgcgctcc ttgggatcgg ccagcaactc ctcggcggtc gccgcgtcct
     6181 cttgagggtt ggttcccctc gggcgggtgt ccgcgatcgg ccgcaaggtc accgtctcgc
     6241 ccgccagccg caccaggatc tccggcgacg ctcccacgac gtgatggtcg ccgaagtcga
     6301 agtagtacat atagggcgat ggattgacgc cgcgcagggc gcggtagagc gcgagcggcg
     6361 gcgcgtcgaa ggcgcgcgag gtgcgctgcg agatctgcac ctgcatgacg tcgccggcaa
     6421 agatatattc cttcgcgcgc cgcaccgcgt cgaggaaccc ggcctcggtg aaggagcagt
     6481 cgagcttcgc ttcctttggt ttttgatttt caaagaaacg ctcgtccggg aggggtcccg
     6541 cgaggcgcga gcgcagatgc tccaggcggg ctttcgcgtt gcgctccgcg tcgggctgcc
     6601 gcggatcggc gtacaccacg aggtagagct tgccgaggac gttgtcgacc accgcaagct
     6661 cgtccgagac cagcagcagc atgtccggcg tgccaagcgc atcgggtttt tcttctttca
     6721 gcgcgttttt ctcgatattc cgcacgctct cgtagccgaa gtagccggcg agcccgccgc
     6781 cgaaacgcag cgccgccggg accggcgcga cgcgatggcc ggcgagccac gcgcgcgcgt
     6841 actcgaacgg atcggcctcg acgctttcca ggcagacgtc ggcgccgcgc gcgtcgcgca
     6901 acaggcgccg caccgtcgta ccgcgcgccg tggttctcgc ctctatccgc tcggcgcagg
     6961 cgaggccgat gaacgaatag cgcccgaagc gctcgccgcc gaccaccgat tccaggagat
     7021 atgagtaggg cccgttggcg agcttcaggt agaccgctag cggggtatag aggtcggcgc
     7081 gcgcctccgc cacgaccgga atgcgggtgt agctcaactc aaacgggtac gcatctcgcg
     7141 aatggtcgcg gcgtaatcct tggaaccgaa aatcgcggag ccagcgacga aggtatcggc
     7201 gccggcgcgc gcgatctcgc cgatgttgtc gatcttcacg ccgccgtcga cctcgagcca
     7261 gatctgcttc ttgagtgcgt cgatgcggcg ccgcacttca gtgatctttg gcagcaccga
     7321 gcggatgaac tgctggccgc cgaagccggg gttcaccgac atcagcagca cgagatcgag
     7381 cttctccagc gtgtggtcga gccaggagac cggcgtggcg ggattcagca ccagcccggc
     7441 cctgcatcca tgctctttga tgagggcgat ggtgcggtcg acgtgatcgg aggcttcggg
     7501 atggaagctg atgatgctcg cgcccgcctt ggcgaactcg ggtaccagcg cgtcgaccgg
     7561 cttcaccatg aggtgcacgt cgagtggcag cttgacgtga ggcttgagcg ctgcgcacac
     7621 cagcggcccg acggtcaggt tcggcacgta gtggttgtcc atgacgtcga agtggatcag
     7681 gtcggcgccg gcgcgctcga ccgcgcgcac ttcgtcgccc aggcgggaga aatcggccga
     7741 caggatgctg ggcgcgatgc gaaacgtcat ggccgcgcgg agtttacaat gccgccgtgg
     7801 cacaggagaa gcaggagaag ctctacgagg tcgcggtgaa cgcggcaacg cagtatctcg
     7861 ccgatcagtc cgacgagtcg gccgggcgct acgtgttcgc ctacaccatc accatcaaga
     7921 ataccggcgc cgtcgccgcg cagctcatca gccgccactg ggtcatcacc gacgcgcagg
     7981 gcctggtgca ggaggtgcgc gggctcggcg tggtcggcgc gcagccgctg cttcagcccg
     8041 gcgagcgctt cgagtacacg agcggcgcct cgatcgccac gccggtcggc acgatgcgcg
     8101 gcagctacca gatggtcgcc accgacggca cgcgcttcga agccgcgatc ccggagttca
     8161 cgctttccgt gccccgcatc cttcactaag gttttttggg ccaggtaaac cagacgataa
     8221 gggccacgag tgcaaggacg acgatgatct ccaggtagat gatccacata cgctgcgctg
     8281 ttttgattct aatggtcgcg gcgtgcgcgc ccgcgccggt gaaatgcccg gtctgtccgc
     8341 cgccgaagcc gcagccggag acggcccgat atgaagcgcg cggcttcgcc gaccttccgg
     8401 gctggagccg cgcgcaactc gagcccagct tgcgggcatt catcgccggc tgcgcgcgat
     8461 tcaagagagt ttgtgacctc gcaaagagcc tgccatcggc ggatgaggag gcggcgcgca
     8521 ggttctttga gaccgagttc gtgccgtatg cggtcgtgtc gagcgagagc ggcgacagcg
     8581 ggctcatcac cggctattac gagccgatca tcgacggcag ccgcacgccg agcgcggcgc
     8641 atcgctatcc gatctacggc gtgccagcgg acctggtcgt ggtggatctc ggcgccgtga
     8701 gcgccgagac gcggccgctg cgcctgcgcg ggcgcctcga cggccggcgc ctcgtgccct
     8761 actactcgcg cggcgagatc gacgcgcgcg gcgacttcgc cgcgccggtg atcgcctgga
     8821 gcggcgaccc ggtggagctg ttctttctcc aggtccaggg atcgggacag gtgcggctcg
     8881 agaacggcga gcgcatccgc gtcggctatg ccgaccagaa cggccatccc taccgctcgc
     8941 tcggccggca cctggtcgat cgcggcgagc tggtgctcga gcaggcgtcg atgcaaggca
     9001 tcaaggcgtg ggcggccgtc aaccctcaaa agctgcagga ggcgctcaac cagaacccga
     9061 gttacgtctt cttccgcgag ctaccgccga ccgacggccc gatcggcgcg ctgaatatcc
     9121 cgctgcacgc cgagcgcagc ctcgcggtcg accgccgctt cgtgccgctc ggggcgccgg
     9181 tgtacctcgc gacgaccttt ccgctttcgg aagagccgct cgagcggttg atggcggctc
     9241 acgataccgg cggggcgatc cgcggcgtgg tgcgcgcaga cttctactgg ggcaccggtc
     9301 ccgaggctgg cgcccaggcc ggacgcatgc gccagcaggg aaagatgtgg ctgctgtggc
     9361 ctaccggcga gccgttgccg gccggtccag cagctccttg agatcgtcgg ggcccaggcc
     9421 ccccgacagg cgctcgccat tggcgagaaa cagcgtcggc gtgctgttga cgccgagctt
     9481 gcggccgagc tcgtgcacct tgtcgagcgg attcgggcag ctcgtgccgg cctgcggcac
     9541 cttgggctgc gccgccgcgg caagctcgag ccaggccttc gcgcggtcgg gcgagcacca
     9601 caccgcgcgc gagtggtcgg agttttccgg ccggatcacc gggtacatga agacgtagac
     9661 cgagatgtcg tcgatctgcg cgagcgcgcg ctcgaactgc cggcacgccg gacagtacgg
     9721 gtcggagaac atcgcgagca cgcgcttgcc gtttccgcgc tgcaccttca ccgcgaggtc
     9781 gagcggcagc gactcgaact tgatcgcgtt cagtgcgcgc gtgcgctctt cggtgaggtt
     9841 gcggtcggtg cgcacctcgt agacgcgacc gacgaacacg tgcgtcgcgc tggcgtcggt
     9901 gtagacgatc tgcaccccgc cttcggaggt gcggaagcgc acctcgaaca ggcccgggac
     9961 cggcccgggc tggattccct cgatcttcgc gccgctgagc ttgggctcga gcgccttgcg
    10021 gatctgcgcc tcgttcgcaa gcgcagcatt tacaaaaaaa acggtaatca ggatgaaaaa
    10081 gcgcgcgatc acgtcatcgc ctggcgcatc agcatgctct ttacgaccgg cagccgatcg
    10141 gtcaagttca accccgcatt gcgcagccgg gccgcaaatc ggtcctcggc gccgaacaac
    10201 cggaatagcg aatcaaccat ggtgtccatc gcgagtatag gctcggcgcg cccacgctca
    10261 tagcgccgca gcaggcgcag ctcgcccgga tcgcgctgcg gctcgcggga atgcagcacc
    10321 gcggcgagtg tgcgcgcgtc ctgcaagccg agattcagtc cctgtccggc cagaggatgg
    10381 accacgtggc cggcgtcgcc ggcgagggcc acgcgcggcg cgaccagacg ctcggccgcg
    10441 agccgccgca acggatagct tcgcggcgcg gtgacggtct cgagctcgcc gagcacgccg
    10501 cccgctgcga cctcgaccgc acggcaaagc gcttcgcctt ccaaggcgcc ggtgcttctt
    10561 gcttgctcgg tcggcagcga ccaaaccatc gacacgtggt tacccgggag cggcagcagc
    10621 gcgagcaccg cgccgcgctg gaaccattga taggcgacgt tgccgtgcgg cttgccgcag
    10681 gcgaagttcg ccaccaccgc gctctgaccg tagtcacggt cggtagcccc gatgccggcc
    10741 tgccgccgca cgaaggaatg cgcgccgtcg gcgccgacga tcagctttgc gccgatcctg
    10801 ccgccgtcgt gcatgccgag ggtggcccag tcctcgtcga tgctcaagcc tccacactga
    10861 gccaccagca cgtcggcttc gagatcgcgc cacagcgcgt cctgcagcag cgcgtcctcg
    10921 acgatccacg cgagctgcgg aacgccggcg ctgtaggcgt cgaaatccag cctcgaagcg
    10981 tcgtcgccat gaatgcgcat ggcgtgcacc ggcacgagcc ggtcttcggg gatcgcctgc
    11041 cacgcgccga tcgctttcag gaacgccacg ttgccggggc tcagggcgta gacccgcgca
    11101 tcgaagccgg agccgatggg tgcgggacgc tgctgcgcca ccagcagcac cgacatcccg
    11161 cgcgccgcgc gcgcgagact tgcgccgacc ggaccgccgc cgacgacggc gaggtcgtgc
    11221 cgcccagggt ttataatcgc gtctcgatcc gtggcgaatt agctcagctg gttagagcag
    11281 cggaatcata atccgtgtgt ccggggttcg agtccctgat tcgccaccac taattcagac
    11341 gccggcggtc gacttgatcg ccgcgaccat cgcgtaaggc ttcgcggtct tcggcaggta
    11401 ccacgccgcg ccggccgcga tcgcgcgctc catggtgacc gggtcctcgg tcgccgacac
    11461 cacgatcacg cgcaccttcg ggaacgcgct gcgaaatcgc agcagcgcct cgatgccgga
    11521 gtagccgggc aacccgaggt cgagcaacgc gagccggaag tccgcgagtc ccatgccgat
    11581 tgcgatcgcc gcgccgagct cgctctcggc gtgcacgctc gcgccgggga tcgccttgcg
    11641 caccatcgcc gtcagcgtct cgtggatgat cgggtggtcg tcgatgatca ggacgtccat
    11701 ggctagggct gtgtcttgta ctcggactct gccttcggcc gcgtgccgac aaagcgggcg
    11761 agaaacaccc cggcctcgta gagcaggcac atcggcaccg ccagcatgaa ctgcgacagc
    11821 acgtccggcg gcgtcaccac ggcggcgacg acgaatgcgc ccacgatcac gtagggccgc
    11881 gcctcgcgca gcttctccac cgtgacaacg cccatccgca ccagcacgat caccacgatc
    11941 gggatctcga aggtgatgcc gaaggcgagg aacatggtga tcacgaagct gaagtacgcc
    12001 tcgatgtccg gcgccggggt gatcgacttg ggggcgaagt cgtggatgaa ggcgaacacc
    12061 ttgccgaaca cgaagaagta gcagaacgcg acgccgagga agaacagcac cgtgctcgcg
    12121 acgatcagcg gcatggcgag cttcttctcg tgctcgtaga gccccggcgc gacgaaggcc
    12181 cacgcctggt agagcacgta gggcagcgcc accatgaacc ccatgagcgc ggtcaccttc
    12241 accggcacca tgaacggcgt gatcacgccg gtcgcgatca tcttggagcc ctccggcagc
    12301 gcgttcatca gcggcagggc gagcaggtcg tagatatagc ccgagcccgg gtagagaaac
    12361 agcaccacga agatcgcgag caccgcgccg accgcgcgca ccagccgcgc gcgcagctcg
    12421 atcagatggg agatgaagct ttcctggtcg ctcatccgac cttcgcctgc tcgggtttct
    12481 tggattcctc gaccgcgatt tcagagggat tggcctgttc ggccgcaggc ggaggcggat
    12541 cccccacgac ggcttcgacc gacttgttca gctcggtctc cgtcttggtc atctcgcttt
    12601 gcaccgagct ctcgaagttg ctcgccgcct gctgcatcga gtcgcgcatc ttgcgcagct
    12661 cgtcgagctc gatctcgcgg ttgatgtccg ccttcacgtc ggacacgtag cgttgcagcc
    12721 gcccggcgag gtggccgacg gtgcgcgcca tgcgcggcag cttctccggg ccgatcacga
    12781 tgagcgcgac caccccgatc acgagaagct cggtgaagcc tatgtcgaac atggcggcgt
    12841 agaaaagtag agaagcagaa taaaaaggcg ccgcgcgggc gccttctcaa cacaagccgg
    12901 gaagtcgctc aggccttctg ctcggtcttg cccttgactt cgccttcgat cgtcttcccg
    12961 ctgacctgcg gtgcgggatc ggccggcttc tcgccgcctt cgcgcatccc gtccttgaat
    13021 ccgcgcaccg cgccgccaag atccgtgccc atgttgcgca gcttcttggt gccgaagatc
    13081 agcatcacga tcaccaggac gatcagccaa tgccagatgc tgagcgaacc catatttgcc
    13141 tcctatcgtt gcctctggag catcggcccg agaggatccg ggccgcccag gacgtgcata
    13201 tggacatgat acacctcctg atgccccacc cggccggtgt tgacgatggt gcggaagccg
    13261 tcggcggcgc ccgcctcgcg cgcaaggcgg ccggcgacgg caagcatctt gcccagcgcg
    13321 gcgtggtgcg ccgcaccggc gtcatacagc gtcgcgatgt gcaccttggg gatgaccagc
    13381 aggtgaaccg gcgcctgcgg gcgaatgtcc gggaaggcga ggacttcggc gtcttcgtaa
    13441 attttctttg ccgggatctc gccgcgcacg atcttgcaaa aaatgcaatt cgggtcgttc
    13501 attcttccct catttgtccc tccgggcttt ctcgtcgatt ccggagatgc cctcgcggcg
    13561 ctgcatctcg gcgatcacgt cgcccggccc gaggccgtgg cgcgcaagca cgatcatgca
    13621 gtggaaccag agatcggtca cctcgcgcac caggtgcagc cggtcgccgc tcttcgccgc
    13681 gagcagcgtc tcggtcgcct cctcgccgat cttcttgagc atcgcgtcgt cgccctgcgc
    13741 gaacagcttc gcgacgtagg acttcgccgg atcgccccct tttctttcat tgatggaagc
    13801 agcaatgcgc tccagcgtct ctgcttgtcc gagtttcatt ttttgtagat gagcgaggga
    13861 tccttgagca ccggatcgat cgtcacccac ctcccgttct ccagtttgcg gaaaaagcag
    13921 ctctcgcgtc ccgtatggca ggcgatgccg ccgacctgct cgaccttgag caggatcgcg
    13981 tcggcgtcgc agtcgatgcg cacctcgagc accttctgta cgtggccgga ttcttcgccc
    14041 ttgcgccaca gcctcttgcg cgagcgcgac cagtacaccg cccgcttcgt ttccgcggtc
    14101 tctttcagcg cgtcgcggtt catccaggcg acggtcagca ctcttccgct agcagcgtcc
    14161 tgcgccacga ccgcgaccag cccgtcggcg ttccagggaa cttcatccag aaagttcaaa
    14221 ggcgaacctc tatgccgcgc gcgcgcatgt gctccttcgc cgcgcgcacc gtgaagtcgc
    14281 caaaatggaa cacgctcgcc gcgagcaccg catcggcgcc gccggcaagc acgccctcgg
    14341 cgagatgctc catcgagccg acgccgccgg aagcgatgac cgggacgccc accgcatcgg
    14401 cgaccgcgcg ggtgaggccg aggtcgaagc cgtcgcgcgt gccgtcgcga tccatgctgg
    14461 tgagcaggat ctcgcccgcg cccgcgtgcg ccatgcgccg cgcccattcg accgcgtcga
    14521 gcccggtggc cttgcggccg ccgtgggtaa agacctccca gccggtggaa ttcttcttcg
    14581 cgtcgatggc gacgacgatg cactggcagc cgacgatgcc cgcggcgtcg cgcacgagct
    14641 gcggattctg caccgcggcg gtgttgatcg agaccttgtc cgcacccgcg ttgaggaggc
    14701 ggcgcacgtc gcccacctgg cgtacgccgc cgccaacggt gagcggaatg aacacgcgct
    14761 cggcgaccgc ctcgatcacg tgcagcagca tgtcgcgctc gtccgagctc gccgtgatgt
    14821 cgaggaagca gacctcgtcc gcgccttcgc cgtcgtagcg cgcggcgatc tcgaccgggt
    14881 cgcccgcgtc gcgcaggccg acgaagttca cgcccttcac cacgcgcccc gcggtgacgt
    14941 ccaggcaagg aataacgcgc ttcgcgaggg gcactgctat tctttcccga gggccttgtt
    15001 cgcggcttgc tgggccttct tcaggtcgat cctgccttcg tacagcgcgc gcccggcgat
    15061 cacgccgatg atcccttccg gcaccagctt ctcgcacacg gcctgcacgt ccttcaggct
    15121 ggcgagcccg cccgaggcga tgatcggcgt cttcagctgc tgggcgagct tgaacgtggc
    15181 gtcgatgttg acgccgctca tcatgccgtc gcggccgatg tcggtgtaga tgatcgcctc
    15241 gacgccgtat tcctcgaact tcttcgcgag atcgatcacg tcgtggccgg tgagcttgga
    15301 ccagccctcg accgcgacct tgccgtcctt cgcgtcgagc ccggcgatca cgtggccggg
    15361 gaaggcatag caggcatcgg acaggaagcc gggattcttg accgcggcgg tgccgatgac
    15421 cacgtacgtg accccggcgt cgatatagct ttcgatcgca tcgaggtcac ggatgccgcc
    15481 gccgagctgg atcggcaccg cgtcgcccac ggcggcgacg atgtcgcgga tcaccttctc
    15541 gttcttcggc cggccggcga tggcgccgtt caggtcgacg atgtgcaggc gcttcgcgcc
    15601 ctcggccgcc cagtgccgcg ccatcgccac cgggtcctcg gagaacaccg tcgccgtgct
    15661 catgtcgccc tgcttcaggc gcacgcagcg gccgtctttc agatcaatcg cagggatgat
    15721 gagcactgca gtgctcgatt aacgatcagg gacgccagcg cacgaagttg gagaggagtc
    15781 gcaggccggc ggactggctt ttctcggggt ggaactggac ggcaaaaata ttatcccggg
    15841 caatggcgca ggtaaagggc gcgccgtaca ccgcggtggc ggcggtcaac gcggcgtctc
    15901 gcggcgccgg atagtagctg tgcacgaaat agaagcggct caggtccggg atccccgccc
    15961 acagatcgtg cgggcgcgtc tgcgccacct cgttccagcc gatatggggg atctttagcc
    16021 ccgtaagggc gaagcgcggc acgcgcccgg gcagcaggcc gagacccgcc gcgtcgccct
    16081 cttccccgcg ctcgaacagc atctgcagcc cgatgcagat gccgaggaag ggcttgcgcg
    16141 ccgcggcctc gacgaccgcc tcgcgcgccc cgctggcctc gagctggcgc atgcaatcgg
    16201 gaagcgcgcc ctgcccggga acgacgaccc tctccgagga cgaaatcgcc ccgggctccg
    16261 ccgtcaccag gacctcggcc tggggcgcga cgtgctcgag cgccttcgaa accgagcgca
    16321 ggttgcccat gccgtaatcg accaccgcga tccgcatagg aaactcagcc ctggagcgtt
    16381 cccttggtcg aaggcacggc tccggcggcg cgcgggtcgg gctcggcggc catgcgcagc
    16441 gcccgcgcga acgccttgaa catggtctcg cactggtggt gcgcgttgtc gccgcgcaga
    16501 ttgtcgatgt gcagcgtgac ctgcgcgtgg ttgacgaagc cctggaagaa ctcgtgcatc
    16561 aggtcgacgt cgaattcgcc gatcagcgcg cgcgtgaact tgacgtggta ttcgaggccc
    16621 ggccggccgg aaaggtcgag caccacgcgc gacagcgcct cgtcgagcgg cacgtaggcg
    16681 tggccgaagc ggcgcacgcc ggccttgtcg ccgatcgcct tggccaccgc ctggccgagc
    16741 gtgatgccga tgtcctcgac cgtgtggtgc gcgtcgatgt gcaggtcgcc cttcgcctcg
    16801 atgtcgaggt cgagcatcgc gtggcgcgcc acctggtcga gcatgtgctc gaggaacggc
    16861 agcccggtcg aaagcttcgc cgcgcctttc ccgtccaggt tgagcttgac gcggatctgc
    16921 gtctctttcg tgttgcgggt gacttcagcc gatctcattg cagggcttcg cgcaaggcat
    16981 tcagcagtat acggttttca tccggcgtgc cgaccgtgac gcgcaggcag ttcttcagcg
    17041 ccggactgtt gaggttgcgc accagcacac cctggcgttt gagcgcctcg aaggcgcggt
    17101 cggcgtccgg cacgcgcacc aggaagaagt tcgcttcgga gggaaagacc gtgacgccct
    17161 tcagtccctg caaggcttcc cccaggttct tgcgctcgga gcgaatgcgc gccgcctgct
    17221 cctcgaggac ctctagcctc tccagcatga aggtagcggc ggcctgcgtc agcgcgctca
    17281 cgttgtaggg cgggcgcacc ttgtcgagct gcgcgaccca ctccggccgg ccgaccaggt
    17341 agcccagccg gatgccggca aggccgagct tggacacggt gcggatcacc accaggttgc
    17401 cgaactccgc gagccgcggc aggaagctct tgcgcgcgaa cacgtggtac gcctcatcta
    17461 ccaccaccag tccgctggaa gcgcggatga tcttgaccac gtctgcttcc gggtacagca
    17521 cgccggtggg attgttcggg taggccagga acacgagcgc tggcttctcc gcctgcatgc
    17581 gcgcgatgaa agcatcggcg tcgagcgaaa aatcctcgcg cagcggcacc ggcacggcgc
    17641 gcatgccgct gaaggtcgcg cccatcgagt acatcacgaa cgtcggcgac gggaacatca
    17701 tcgccgcacc cggccgcgcg agcgcggtga tcagcatctg gatcagctcg tccgagccgt
    17761 tgcccaggag gacctccatg ccggccggca cgctcatgcg ccgtgcgatc gcttcgcgca
    17821 gctcttgcgg gctgggcgtg ggatagcgat tgacgtcgac gcgcgcgagc accgccgcaa
    17881 gctcgcgccg cagcccgtcg ggcagcgtgt aggggttttc catcgcgtcg agcttcacca
    17941 tcccctcaga ggaggggacc ggataggcct tgagcgcgag aatctccggc cgaacaagat
    18001 cctcaggttt catttgaatc tcatctcggc ggcgcgcgcg tgcgcggtga ggccctcgcc
    18061 ttcggcgagc gtcatcgcga cgcggcccag cttggccgcg gcggattgcg aaacccggat
    18121 cacgctggtt cgcttctgga agtcgtagac gccgagcggc gacgagaacc gcgcggtgcc
    18181 cgcggtaggc agtacgtggt tcggcccggc gcaatagtcg ccgatcgctt ccgagctcca
    18241 gcggccgaga aagatggcgc ccgcgttcct gactttttca acgaggcctt ccggattttc
    18301 caccgagagc tcgaggtgct cgggcgcgat gcggttcgcc accgcgcacg cctcttccag
    18361 gtcgcgcgtc aggatgagcg cgccacgcgc cttcagggac gcggcaatga ctttccgcct
    18421 tggcatttcg tccagcagtt tccgaatgga agcggcaacc tcatcgaggt acccctcgtt
    18481 cggtgacagc aggatggcct gggccgattc gtcgtgctcg gcctgcgaga acaggtccat
    18541 cgcgacccag tcggcgggcg cggctccgtc cgcgatcacc aggatctcgg aggggccggc
    18601 gagcatgtcg atgccgacct cgccgaacac ctggcgcttt gcctcggcga cataggcatt
    18661 gcccgggccg acgatcttgt ccacccgcgg gatcgatttc gtgccgtagg cgagcgcggc
    18721 gatcgcctgc gcgccgccga tgccgaggac gcggtcgaca ccggcgagcg cggctgccgc
    18781 cagcaccagc gcgttggggt tggggctcgc catcaccagc tcgcgcaccc cggccacttt
    18841 cgccggcacc gcattcatca ggaccgagga cgggtaggcc gccttgccgc cgggcacgta
    18901 caggccgact cgctcgagcg ggctcagcct ctggccaagg cgcgtgccgt cggcatcggt
    18961 gaactcccac gacttctgca gctgcttttc gtggaacgcc cggatgcggt cgtgcgcggc
    19021 gcgcagcgct tgagcctgtt ccgcggggat ggcctcgagg aatttttctg acaccgcgta
    19081 ctgcttcggc gcgacaccgt cgaatttctt cgtgtaatcg cgcaccgcgg cgtcgccgcg
    19141 cttgcgcaca tccgcaagga tcctgcgcac cgctttctgc accgccgggt cctgcgtggc
    19201 ctcgtagcgc gtgagcgccg acagcttttc cgaaaaattc ctttgttggg tcgaaagacg
    19261 acgcagtttc atcgcgccgc ctcggcgaac gcttcgatca gcggctgcag cgtcgcgcgc
    19321 ttcgtcttga gggctgcctg gttgacgatc aggcgcgccg acaccggcat gatctcctcc
    19381 accgcgacca ggccgttcgc cttcagggtc ttgcccgtcg aaaccaggtc cacgatcgcg
    19441 tcggccagcc cgacgagcgg cgcgagctcc atcgagccgt acagccggat gaggtcgatg
    19501 tgcacgccct tggcggcgaa atgctcgcgc gccgtcttga ggtacttggt ggcgacgcgc
    19561 aagcgcgcgc cctggcgcac cgccgcggcg tagtcgaagc ccttgcgcac cgcgaccatc
    19621 atgcgacagg cggcgatgcg caggtccacc ggctggtaca ggccttcgcc gccgtgctcc
    19681 gcgagcacgt cgcgcccggc gatgccgaga tcggcgccgc cgtgctgcac gtaagtggga
    19741 gtgtcggcgg cgcgcaccac gaccagccgc aggtcacggc ggttggtgcc gatcaccagc
    19801 ttgcgcgagg tttcggggtt ttccttaggc cggatgccga cgcgcgcgag cagcggtgcg
    19861 gtctcgtcga agatccgccc cttcgacaac gcaatgctga tgctcgccat gcgccatatc
    19921 cggaaactct agttcaatga ttcggtgggg cgcgcattct accgcaatcc ttagcggatg
    19981 cgcaccacgc gcgcgccgag cgaggagagc ttggtctcga gtgcttcgta gccgcggtcg
    20041 aggtgataga tgcggtcgac gacggtatcg ccgtcggcga ccaggccggc gatcaccagg
    20101 cctgccgaag cgcgcaggtc ggtcgccatg accttcgcgc cctgcagccg ctcgacgcct
    20161 ttcacgaccg cggtattgcc ttccagccgg atgtcggcgc ccaggcgctg catctcgagc
    20221 gcgtgcatga agcggttctc gaagatggtc tcggtgataa gcgcggtgcc acgcgcgacc
    20281 gtggcgagcg ccatgaactg cgcctgcatg tcggtggcga agccgggata cggcgcggtg
    20341 cggatgctga ccgcatcgga tttcctgtcg gcttcaagct cgatcgacga gccctggatc
    20401 ctgatcttgg caccggcctc gcgcaacttg tccagggtgg catcgagcga atccggagcg
    20461 gtgccggtga ggcgcacctt gccgccggtg gcggccgcgg ccgccaggta ggtgcccgtc
    20521 tcgatgcggt cgggcatcac gcggtactcg gcgcccccga gcgagcgcac gccctggatg
    20581 cggatcgtgt cgctgcccgc gccgtcgatc ctcgcgccca tcgcggtgag gcagcgcgcg
    20641 agatccacga cctccggctc gcgcgccgcg ttctcgagca gcgtctcgcc ctcggcgagc
    20701 gacgcggcca tcatcaggtt ctcggtgccg gtcacggtca cgaggtccat gacgatgcgc
    20761 gcgccgcgca gccgctccgc gctcgcgtgc atgtagccgt gctcgaccgc gatcgtcgcg
    20821 cccatggcct cgaggccctt gacgtgctgg tcgaccggcc gcgcgccgat ggcgcaaccg
    20881 ccgggcaggg agaccttggc gcggccgcag cgcgcaagga gtgggcccag caccagcacc
    20941 gaggcgcgca tcgtcttgac gagctcgtag gatgccgtcg gttccttgat ggcggcggcg
    21001 tggagggcga cctgaccttc gggggagctc tccacacgca ggcccatttc ccgcaggagc
    21061 ttcgccatgg tcaagacgtc gcgcaagtgc ggcacgttgc tgatgttcaa tggcttcgca
    21121 gtcagcagcg cggcgcacat gatcggcagc gccgcgtttt tcgcgccgga gacgcgcacc
    21181 tcgccctcga gcggccggcc gccggtgatc tttagcttgt ccattgcgac gttctagcgc
    21241 ccaaaaatgc gtttcttatg gcgttccgct ctttttccat tcgtcgggcg tcagcgtctg
    21301 catggacagt gcgtggattt cttcgcgcat gcgatcgccc agggccttgt agacgagctg
    21361 gtggcgttgc acgcgattgc ggccggcgaa ctgcgccgac accaccagcg cctggaaatg
    21421 ctggccgtcg ccgacgacct cgacatgatc gcaggccagc cccgcttcga tgccgctttt
    21481 cacgctttcc ggcgtcacca ttttctaggt ccttaacctg taccctgatt tgagcagcaa
    21541 gaggcacaaa gccgccaccg caccgaaggc gaccgcgacg accgcgagac tgactccggg
    21601 cgggaagtcg gacgaagcga aaaagccgta acggaagccg tcgatcatgt agaagaaggg
    21661 gttcgcgtga gacaggccgc gccagaacgc gggcagcgac cggatggagt agaacacgcc
    21721 cgagagcatg gtgagcggca ggatgacgaa gttctggaac gcggcgagct ggtcgatttt
    21781 ctccgaaacg atgccggcga ggaggccgag cgcgccgagg agcgccgcgc cgaggagggc
    21841 gaaggccgcc gtccacagcg gcgcctccag gcgcaggtcg acgaaccacg cggtgacggc
    21901 aagcacgccc gccccgacgg cgaggccgcg cgccaccgcc gcggcgacat aggcccagaa
    21961 gaattccgcg tacgagatgg gcgcaagcag gatgaacacg atgttgccgg tgatcttgga
    22021 ctggatcagg ctggaggagc tgttggcgaa ggcgttctgc agcacgctca tcatcaccag
    22081 gcccggcacc aggaacgcgg tatagcgcac gccgtcgaaa acctggaccc ggccttccag
    22141 cgcctggccg aaaatcacga gatacaggag cgaggtgagc accggcgccg ccacggtctg
    22201 cacgctgacc ttgacgaagc gcagcagctc cttggcgaac aaggtccaaa agctcatgac
    22261 cgggtgagct ggagaaaaac ctgctcgagg tcgggccgct cgagctcgag gtcggcgatc
    22321 tcgacgccct gctcgcgcag gcgggcgagc tccgcttcga gctggccgaa accctggagc
    22381 tcgagggtga actgccggcc gtcgcgcgcc accacgcgca ccgtcgagcg cgagccggcg
    22441 aagctcgcca gaagattgcg cgtcgagtcg agcgccacga tgcgccccgc cttgagcatc
    22501 gcgatccggc tgcagtgcag ctcggcttcc tccaggtagt gcgtggtgag gacgatggtg
    22561 tggccgtcgc ggttgaggcg ggtaatgaac tgccacagcc cctggcgcag ctcgacgtcg
    22621 accccggcgg tgggctcgtc gagcacgatc accggcggct tgtgcaccag cgcctgcgcc
    22681 acgagcacgc gccgcttcat cccgccggag agcgagcgca tgttggcgtc cgccttggcg
    22741 gacagatcga ggtgatgcat cacctcgtcg atccacgcct cgttgccgcg gattccgaag
    22801 tagcccgact ggatgcgaag cgtctcgcgc accgtgaaga aaggatcgaa aaccagttcc
    22861 tgcggcacca caccgagcat gcgccgggcg cgccggtagt ccgcgatcgc gtccgcgccc
    22921 atcaccgaca ccgagccggc atcggcgcgc gcgagtcccg caatcacgtt gatcagcgtg
    22981 gtcttgccgg cgccgttcgg gccgagcagg ccgaagaact cgccctgctc gatcgccagg
    23041 ctcacgcccg acagcgcgtg caccgcgccg tagcgcttct ccacgccgcg gacttcgatg
    23101 gcgttcaggt ttttaaaggc agaagttctt ccacgccata caggcgggac agggtctgca
    23161 gggcctcggg cgggttgacg aacgcgagct cgcgcttcgc ggcgcgcgcg tcgcgcagcc
    23221 aggcgagcgc cagcgcgagc agcgccgagt ccatctcggt aacctcgccg aggtcgacgg
    23281 tgcgcacgcc ctcggcgaga tgctggcggc ccgcctcgag caactgcgcg gcggtggcga
    23341 gcgtgaccgg cccgctgacg gtgaggcggc ggccttcgcg ccggatcact tcttgtcctg
    23401 cttggcgagc gagcggttct tggtctgcag gtccttgatc aggccgtcga tgcccgagtt
    23461 gcgcacggtg tttgcgaact cggtgcggta gttggcgacc aggctcacgc cgcccaccat
    23521 cacgtcgtag cacttccagc cgttcgcggt cttttccatg ctgtagtcga gggtcaccgc
    23581 ctgcgcgccg ggctgcagca cgcgcacgtt gaccgtaacg tcggtgtcgg tcggcttggc
    23641 gcgcagcggc cggaagtcga acttctgctc gctgtaggcg gcaagcgcgc tcgcgtaggt
    23701 gcgcacgaga agcgtcttga actcctcggt gaggcgcttc ttctgctccg gggtgctc
//
LOCUS       DATBSZ010000111         5307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_15885, whole genome shotgun sequence.
ACCESSION   DATBSZ010000111 DATBSZ010000000
VERSION     DATBSZ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15885"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..832)
                     /locus_tag="VIG70_07345"
     CDS             complement(128..832)
                     /locus_tag="VIG70_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY8250405.1"
                     /translation="MNVSVWRGGAEGRFQQFEVPRHPSQTVLDVVTYIQRTLDPTLAY
                     RFACRVGMCGSCAMMVNGVPRWTCRTHVDRVVEKDALTVAPLRNLPVIRDLVADMREF
                     FDKWARAKGEFRGRQTKQDDFAKVPPQSEERVLADKGIECIGCAVCYAACDVVKWNPD
                     YLGPAALNRAWTLVKDVRDRGKLERLRAVSGDAGCHACHSHVSCTERCPKHLSPTAAI
                     AGLKREVAKAVLLGEL"
     gene            complement(829..2001)
                     /locus_tag="VIG70_07350"
     CDS             complement(829..2001)
                     /locus_tag="VIG70_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEY8250406.1"
                     /translation="MQNLSKSSFWGYKRENGRVGVRNHVLILPVDDLSNAAAQAVENN
                     VKGTLAIPHPYGRLQFGADLELHFRTLIGAGSNPNVAAVVVICIEDTWAKKVADGIRA
                     SGKPVAHFGIEQHGDHDTIMRASKAAREFLQWASELRREERPLKDLWVSTKCGESDTT
                     SGCGANPTVGNAFDKMYPHGVTMVFGETTELTGGEHLVAARCRNEEVRKKFQFVFDRY
                     QQVIERHKTSDLMDSQPTKGNIEGGLTTIEEKALGNIQKIGRKCLVDGVLDKAEVPTG
                     PGLWFMDSSSAAAEMVTLCAAAGFAVHLFPTGQGNVIGNPILPVIKLSANPRTVRTMS
                     EHIDVDVSGLLRKELTPDQAGDKLLECMFRTANGRLTAAEALGHREFVLTRLYESA"
     gene            complement(2013..2294)
                     /locus_tag="VIG70_07355"
     CDS             complement(2013..2294)
                     /locus_tag="VIG70_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEY8250407.1"
                     /translation="MIHFVVHEEGDGVGVVVVEGVKSGQPLSGWIMEDDKDVAVTAKN
                     DIPIGHKIALKDYQPGDTVIKYGVDIGKVVKPIAKGEHLHVHNLKTKRW"
     gene            2314..3843
                     /locus_tag="VIG70_07360"
     CDS             2314..3843
                     /locus_tag="VIG70_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY8250408.1"
                     /translation="MYGRENARRRFLLSLGALAVSTHVRAQPRFASYPFTLGVASGYP
                     SPESVVLWTRLTGELAPQAIPVRWEVFADESLRTAVASGQALADPLWAHSVHVEAPGL
                     EPGRWYWYRFSAGDAQSPVGRTRTAPRAGAPAPRLRFAFASCQQYEQGYYGAYRHMVA
                     DAPDLVAFLGDYIYESSWGRQLVRSHGSAEAHTLEDYRARYALYRSDPDLQAAHAACP
                     WIVTWDDHEVDNDYADDRSEDGMEREAFLARRTAAYRAYYEHMPLPPRMRPEGPNMRI
                     YTHLDWGALARFYVLDDRQYRSWHACPRPQRRAGSNTIDIERCPRLPAPGRSMLGRAQ
                     EQWLDRELGESRAAWNLIAQQTRMAQFDYKPGPGRSAWTDAWDGYPAARTRLLTSLSS
                     ERVRNPVVIGGDVHTFYANQLKLDFDDPQAPVIAAEFVTSSITSESWAQERLNQYLPD
                     NPHVLLAESRSRGYTRVDVTPERFQVDLRGMETVQRRDAPCSTLVSFVVDDGIPGPVR
                     A"
     gene            complement(3851..4162)
                     /locus_tag="VIG70_07365"
     CDS             complement(3851..4162)
                     /locus_tag="VIG70_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250409.1"
                     /translation="MAAVKALLLGVLLLAGCSSNTAWKVSVGDPNPGAQVQVHSTGDL
                     AALFGLAFLAAGAYSAASGDLAYTYGAAQRAPEMAPDRRVSEQDCTKPIDYSLGNIRC
                     K"
     gene            complement(4164..5105)
                     /locus_tag="VIG70_07370"
     CDS             complement(4164..5105)
                     /locus_tag="VIG70_07370"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY8250410.1"
                     /translation="MNPEVRRAAQILRAGGLVAFPTETVYGLGADAASEKAVARLYQT
                     KRRPAAHPVIVHFENSEKAFAWAREIPAAARKLAEKFWPGPLTLILKRSALAKDFVTG
                     GQDTVGLRVPSHAIAHQLLKEFGGAVAAPSANRFGFVSPTTAAHVREDLGKDVDLVLE
                     GGPSEVGIESTILDLSGAAPVLLRPGHLSKEQLKQVLGVEVFEKTEASPRHSGGLERH
                     YAPRTPARMVPTYGLDKAIAELKARVAVLAFSRPDERVDYWLRMPREPQAYAQRLYAA
                     LRELDAAGCERILVEAPPDSPEWTAVRDRLRRACGQA"
     gene            complement(5102..>5307)
                     /locus_tag="VIG70_07375"
     CDS             complement(5102..>5307)
                     /locus_tag="VIG70_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEY8250411.1"
                     /translation="LDSLLSTVQMPKGIPVATFAIGEAGAANAALFAVALLALSDKGL
                     ASKLEEFRRKQTEAVLKGAPLKL"
BASE COUNT          813 a         1866 c         1722 g          906 t
ORIGIN      
        1 atctcggccg ccgtcagccc gccctggacc gcgtagacga tggtgacgag atgcacgacg
       61 acgcacactg ccagcacggc cgcgctcgcg cgctgcgcga tccacagggc cgcctgcgtc
      121 ttgaagctca aagctcgccc agcaggacgg ccttcgccac ctcgcgcttc agcccggcga
      181 tcgcggcggt gggagagagg tgcttcgggc agcgctcggt gcagctcacg tggctatgac
      241 aggcgtggca gccggcgtcg cccgagaccg cgcgcagccg ctcgagcttg ccccggtccc
      301 tgacgtcctt gaccagcgtc caggcgcggt tcagcgcggc cgggccgagg tagtccggat
      361 tccacttcac cacgtcgcag gcggcatagc acaccgcgca accgatgcat tcgatgcctt
      421 tgtcggcgag gactctctct tcggactgcg gcggcacttt cgcgaaatcg tcctgcttcg
      481 tctggcggcc gcggaactcg cccttcgccc gcgcccactt gtcgaagaac tcgcgcatgt
      541 ccgcgaccag gtcgcggatc accggcaggt tgcgcagcgg cgcaacggtc aacgcgtctt
      601 tttcaacaac ccgatctaca tgcgtgcggc aggtccagcg cggcacgccg ttcaccatca
      661 tcgcgcacga gccgcacatc ccgacccggc acgcaaagcg gtaggcgagc gtcggatcga
      721 gcgtgcgctg gatgtaggtg acgacgtcga ggacggtctg gctgggatgg cgcggaacct
      781 cgaactgctg aaagcggcct tccgcgcccc cgcgccagac ggaaacgttc aagccgattc
      841 gtacaaccta gtgagaacga attcgcggtg cccgagcgcc tcggctgcgg tgaggcggcc
      901 gttggcggtg cggaacatgc actcgagcag cttgtcgccg gcctgatcgg gcgtcagttc
      961 ctttcgcaga aggcccgaaa cgtcgacgtc gatgtgctcg gacatggtgc ggacggtgcg
     1021 cggattcgcg ctcagcttga tcaccggcag gatcgggttg ccgatgacgt tgccctgccc
     1081 ggtgggaaag agatgcaccg caaagccggc ggcggcgcac agggtcacca tttccgccgc
     1141 ggcggaggac gaatccatga accacaggcc cgggcccgtc ggcacttccg ccttgtcgag
     1201 aaccccgtcc accaggcatt tcctgccgat cttctggatg ttgccgagcg ccttctcctc
     1261 gatcgtggtc aggccgccct cgatgttgcc cttggtcggc tgcgagtcca tgagatcgct
     1321 cgtcttgtgg cgctcgatga cctgctggta gcggtcgaac acgaactgga acttcttgcg
     1381 cacttcctcg ttccggcagc gcgccgcgac caggtgctcg ccgccggtga gctcggtggt
     1441 ctcgccgaac accatggtga cgccgtgggg gtacatcttg tcgaacgcat tgccgacagt
     1501 gggattggcg ccgcagcccg aggtggtatc ggactcgccg catttcgtcg atacccagag
     1561 gtctttgagc ggccgctcct cgcggcgcag ctcgctcgcc cattgcagga attcgcgcgc
     1621 cgctttcgac gcccgcatga tggtgtcgtg gtcgccgtgc tgctcgatgc cgaagtgcgc
     1681 caccggcttg ccgctcgcgc ggatgccgtc ggcgaccttc ttcgcccagg tgtcctcgat
     1741 gcagatcacc accaccgcgg cgacgttcgg gttggagccg gcgccgatga gggtgcggaa
     1801 atgcagctct aggtcggcgc cgaactgcag ccggccgtac gggtgcggaa tcgcgagcgt
     1861 ccccttgacg ttgttctcca cggcttgcgc ggctgcgttc gagaggtcgt ccaccggcag
     1921 gatgaggacg tggttgcgca cgccgacgcg gccgttttcc cgcttgtagc cccagaagct
     1981 gctcttggaa aggttctgca ttttttcccc ggctaccagc gtttcgtttt caggttatgc
     2041 acatgcagat gctcgccctt ggcgatgggc ttcaccactt tgccgatgtc gacgccgtac
     2101 ttgatcaccg tatcgccggg ctggtagtcc ttgagcgcga tcttgtggcc gatcgggatg
     2161 tcgttcttcg ccgtgaccgc gacgtccttg tcgtcctcca tgatccagcc gctcagcggc
     2221 tggccggatt tcacgccctc gacgacgacg acgccgacac catcaccttc ttcgtggaca
     2281 acgaaatgaa tcatggagcg ttattctatt cgtatgtacg ggagagaaaa cgcgcgccgc
     2341 cgcttcctgc tgtcgctcgg cgccttggcc gtttcgacgc acgtacgggc gcagccgcgg
     2401 ttcgcgtcct atccgttcac gctcggcgtc gcctcgggct atccgtcgcc ggaaagcgtc
     2461 gtcctctgga cgcgcctcac cggcgagctc gcgccgcaag cgatcccggt acgctgggag
     2521 gtttttgcgg acgagtcgtt gcgcacggcc gtggcgagcg ggcaagcgct tgccgatccg
     2581 ctctgggcgc actcggtgca cgtcgaagcg ccggggctcg agcccgggcg ctggtactgg
     2641 taccgcttca gcgccggaga cgcgcaaagc ccggtcgggc gcacgcgcac cgcgccgcgt
     2701 gccggagcgc cggcgccgcg cctgcgcttc gcattcgctt cgtgccagca atacgagcag
     2761 ggctactacg gtgcgtaccg ccacatggtc gcagacgcgc ccgacctggt cgccttcctc
     2821 ggcgactaca tctacgaatc gagctggggc cggcagctcg tgcgcagcca cggctctgcc
     2881 gaggcccaca cgctggaaga ttaccgcgcg cgctacgcgc tctaccgctc cgatcccgac
     2941 ctgcaggccg cgcatgccgc ctgcccctgg atcgtcacgt gggacgacca cgaggtcgac
     3001 aacgattacg ccgacgaccg gtccgaggac gggatggagc gcgaggcgtt cctcgcgcgc
     3061 cgcaccgcgg cgtatcgcgc ctactacgag cacatgccgc tgccgccgcg catgcgtccc
     3121 gaaggaccga acatgcgcat ctacacgcat ctcgactggg gcgcgctcgc gcgcttctac
     3181 gtcctggacg accggcagta ccgctcatgg cacgcctgcc cgaggccgca gcgccgcgcc
     3241 ggctccaaca ccatcgacat cgagcgctgc ccgcgtcttc cggcgcccgg acgctcgatg
     3301 ctcgggcgcg cgcaggagca atggctcgac cgcgagcttg gcgagtcgcg cgcggcctgg
     3361 aacctgatcg cccagcagac gcgcatggcg cagttcgact acaagcccgg ccccggccgc
     3421 agcgcctgga ccgacgcctg ggacggctat cccgccgcac gcacgcggct tctcacgtct
     3481 ctctcttcgg agcgggttcg caaccccgtc gtgatcggcg gcgacgtgca cacgttctac
     3541 gccaatcagc tcaagctcga cttcgacgat ccgcaagcgc cggtgatcgc ggccgaattc
     3601 gtcaccagtt cgatcacctc ggagtcgtgg gcgcaggagc gactgaatca atacctgccc
     3661 gacaatccgc acgtcctcct cgccgaaagc cgctcccgcg gctacacgcg cgttgacgtg
     3721 acacccgagc gcttccaggt cgacctgcgc ggcatggaaa cggtccagcg gcgcgacgcc
     3781 ccctgcagca cgctcgtctc gttcgtcgtc gacgacggga tcccgggccc ggtccgggcc
     3841 tagacggctg tcacttgcag cgaatgtttc ccagcgagta gtcgatcggc ttggtgcagt
     3901 cctgctcgct gaccctgcgg tccggcgcca tttctggcgc gcgctgcgcg gctccgtagg
     3961 tgtaggccag gtcgccgctc gccgccgaat acgcgcctgc ggcgaggaag gccaggccga
     4021 acaacgccgc caggtcgccg gtgctgtgca cctgcacctg cgcgccggga ttcgggtcgc
     4081 ccacgctcac tttccacgcc gtattcgagc tgcatcccgc gagcagcaag acacctagaa
     4141 gcagagcctt cacggccgcc atgttacgcc tgcccgcagg cgcgcctgag gcggtcgcgc
     4201 accgcggtcc attccgggga atccggcggc gcctccacca ggatgcgctc gcaaccggcg
     4261 gcatcgagtt cgcgcagcgc tgcgtacagg cgctgcgcat aggcctgcgg ctcgcggggc
     4321 atgcgcagcc agtagtccac gcgctcgtcc ggacgcgaaa acgcgagcac cgccacgcgt
     4381 gctttcagct cggcgatcgc cttgtccagt ccgtaggtgg gaaccatgcg cgcgggcgtg
     4441 cgcggcgcgt agtggcgctc cagcccgccg gagtggcgcg gcgaagcttc ggtcttctcg
     4501 aagacctcca ccccgaggac ctgcttgagt tgctccttcg acaggtggcc cgggcgcagc
     4561 agcaccggcg ccgcccccga aagatcgagg atcgtcgact cgatgccgac ctcgctcggc
     4621 ccgccctcga gcaccagatc gacatccttg ccgagatcct cgcgcacgtg cgcggcggtc
     4681 gtgggcgaga cgaagccgaa gcggttcgcc gacggagcgg caaccgctcc accgaattcc
     4741 ttgagaagct gatgagcaat ggcgtgagag ggcacgcgca atcccacggt gtcctgtccg
     4801 cccgtgacga agtccttggc cagcgcggag cgcttgagga tcagcgtgag cggacccggc
     4861 cagaatttct cggccagctt gcgtgcggcg gccggaattt cgcgcgccca tgcgaaggct
     4921 ttctccgagt tctcgaaatg cacgatcacc ggatgcgcgg ccggccgtcg tttcgtctga
     4981 tagaggcgtg cgactgcctt ttcgctggcc gcatcggcgc ccaggccgta caccgtctca
     5041 gtggggaacg cgacgagccc gccggcgcgc aggatctgcg ccgcgcggcg gacttcggga
     5101 ttcacagctt gagcggcgcg cccttgagca ccgcttcggt ctgcttgcgg cggaactcct
     5161 ccagcttgct tgcgagcccc ttgtccgaaa gcgccagcag cgccaccgcg aagagcgccg
     5221 cgttcgccgc gccggcctcg ccgatcgcaa acgtcgccac cgggattccc ttcggcatct
     5281 gcaccgtgga cagcagcgag tcgagtc
//
LOCUS       DATBSZ010000112         5328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_699389, whole genome shotgun sequence.
ACCESSION   DATBSZ010000112 DATBSZ010000000
VERSION     DATBSZ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699389"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="VIG70_07380"
     CDS             <1..1284
                     /locus_tag="VIG70_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY8250412.1"
                     /translation="LTAPILPTLLRLSAPGVLLVLFQSMVSVGDTYFVGRLGTEPLAA
                     LALVFPLIMLLQMTSAGAMGGGVSSAIARALGSGRAAAARKLVVHALVIAVAMGVGFT
                     ALILLLGESIYHLLGGRGETLGNALRYSNIVFGGAIAVWVANTLSSVLRGSGNMHTPA
                     FTLISAAVVHLPLSATLVGRIGIAGAGIAYVTTFGIAGLVMAAIVFRRSSALRPHRED
                     WTLEWRLFREILRVGAISMLSSAQTVLTAVIITGFVGRYGAAALAGYGVGLRLELLQI
                     PLVFAVGQALVVLVGTHVGAGRPERAKEIAWVGAALAASISLAIGATVWAFPLAWVGL
                     FSTDAAVLNAGAQYLRTVAPFYPFLAVGIALYFASQGAGDVLRPVLAGTARLALVIAG
                     GLVVVSLQGVFAVIAAAMIAFGALTMWIVASARWK"
     gene            1286..2629
                     /locus_tag="VIG70_07385"
     CDS             1286..2629
                     /locus_tag="VIG70_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY8250413.1"
                     /translation="MKESISERLADALARAGEPPREIVEKLLIDVAGLCVAARNTDYV
                     HAALAGWEASGKCTAIGHARALDSAGAAFVNGTAAHGEDFDDTFEGGPVHAGAVIVPA
                     VLALCEREKIPGQMALRGIAVGVELTCRGSTVAPKLIHKAGFHPTAVLGAMGAAAAAG
                     TVLNLTKPEFVNALGIAGSMASGIIEYLAEGTWTKRMHPGWAAQSGIRAADLARNGFV
                     GPRTVFEGEHGFFHGFAHTTSGAWDKLVDGFGRRWVTETVAFKLYPCGTMTHPYIDCA
                     RRLAKKVKVEEIEEIVCEAAEGTLHRLWEPLAAKQRPPNAYAGKFSTPYCIAAGFVLG
                     NAGLDAFTDERVADPRLRALAANVRYEVDPDNPYPNEYTGHVRVRTRDGSVVEERQPH
                     LRGGAHEPLSRADVEEKFKGNCAYGGWSAAQADAFLAWAKNAFGAAPVDLSAFRK"
     gene            complement(2619..3410)
                     /locus_tag="VIG70_07390"
     CDS             complement(2619..3410)
                     /locus_tag="VIG70_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8250414.1"
                     /translation="MNFEVNGKRAFAYTAAHELDASKPSVVFVHGAGLDHSSFALQSR
                     YFGYHGRNVLAVDLPGHGRSEGPPLPSIEAMADWLSAVLKQLKIGKASIVGHSMGSLV
                     ALEFAARHPAQCERIAFVAVGYPMKVAEPFLEAARHDDYAAFDMSTIWGHAAQVPLAA
                     NPNPGMWMYGDALARLERLAPGVLYNDLKACNDYGAGAERAAGVKCAVLFIAGRRDVM
                     TPPRAMKLLQEKIAGAKTVEIAPSGHTLMAEAPDATLDALIGFIS"
     gene            complement(3407..4699)
                     /locus_tag="VIG70_07395"
     CDS             complement(3407..4699)
                     /locus_tag="VIG70_07395"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEY8250415.1"
                     /translation="MAAPKYPALDTLALHAGQSPDPATGSRAVPIYQTTSYVFRDTEH
                     AASLFNMERAGHVYSRISNPTVAVLEERMAALEGGVGSIATASGQAALHLAIATLMGS
                     GGHIVASTALYGGSHNLLAYTLKRFGIDTTFVNPRNLDDWKRSIKSNTRLLFGETLGN
                     PGLDVLDIPSLSTIAHEAKVPLLVDATFTTPVLMKPFEFGADLLYHSATKFLGGHGVA
                     IAGVLVDSGRFDWEASGKFETLTAPYAGFHGMDFEEESGTRAFLLRARREGLRDFGAC
                     MAPMTAFQILQGIETLHLRMPRHVENTRKVVAFLSKNGSVEKVTYPELPEHPDHALAK
                     KLLPHGCGAVFTFEIKGGRPAGKRFIESLRVFSHLANVGDAKSLVIHPATTTHYRMSD
                     EDLKKAGITAGTVRLSIGLEDPQDLIDDLDRALRASGK"
     gene            complement(4772..>5328)
                     /locus_tag="VIG70_07400"
     CDS             complement(4772..>5328)
                     /locus_tag="VIG70_07400"
                     /inference="COORDINATES: protein motif:HMM:NF042855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HEY8250416.1"
                     /translation="MRHEVHLHADIPILEGVSRRQLEQAFAPLLEYLDADGLADVKSL
                     EPEEPGLKFDEKELLLAVCWTGEIGRSFHGALEAALEALGPYCYEAVEIDLTLYHENG
                     EQESKLLFVGPTAQAIHEAQRRCMIEDVAAILARQFAKEEVAQVTTLVNELFDRDWKE
                     KASQQKKPERAFESYTRPSRKNLH"
BASE COUNT          802 a         1749 c         1852 g          925 t
ORIGIN      
        1 ctcacggcgc cgatcctgcc gaccctgctg cgcctgtcgg cgccgggcgt gctgctggtg
       61 ctgttccagt cgatggtctc ggtgggcgat acctatttcg tcggccgcct cggcaccgag
      121 ccgctcgctg cgctcgcgct cgtgttcccg ctgatcatgc tgctgcaaat gacctccgcc
      181 ggcgccatgg gtggcggcgt ctcctcggcg atcgcccgcg ccctcggctc gggcagggcg
      241 gcggctgcgc gcaagctcgt ggtgcacgcg ctcgtgatcg cagtcgcgat gggggtggga
      301 ttcaccgcct tgattctgtt gctgggagaa tcgatctatc acctgctcgg gggccgcggc
      361 gaaaccctgg gcaatgcgct gcgctactcg aacatcgtgt tcggcggtgc gatcgcggtg
      421 tgggtcgcca atacgctctc gagcgtcctg cgcggcagcg gcaacatgca cacgccggca
      481 tttaccctca tcagcgcggc cgtggttcac ctgccgcttt ccgcgaccct cgtgggccgc
      541 atcggcatcg ccggcgccgg aatcgcgtac gtcacgacct tcggcatcgc cgggctcgtg
      601 atggcggcga tcgtgttccg gcgctcgagc gcgctgcgcc cgcaccgcga ggactggacg
      661 ctcgaatggc gcctgttccg cgagattctg cgcgtcggcg cgatctcgat gctgtcttcg
      721 gcgcagacgg ttctcaccgc cgtgatcatc accgggttcg tcgggcgcta cggcgcggcc
      781 gccctcgccg gctacggcgt cgggctgcgc cttgagctgc tgcagatccc gctggtgttc
      841 gcggtcggac aggcgctcgt cgtgctcgtg ggcacccacg tgggtgccgg acgccccgag
      901 cgcgccaagg agatcgcctg ggtcggggca gcgcttgccg cctcgatcag tctcgccatc
      961 ggcgccacgg tgtgggcatt tccgctcgcc tgggtcgggc tgttcagcac cgacgccgcg
     1021 gtgctgaatg ccggcgcgca gtacctgcgc acggtcgcgc cgttctatcc tttcctcgcc
     1081 gtcggcatcg cgctctactt cgcttcgcag ggcgccgggg acgtgctgcg cccggtgctc
     1141 gccggaacgg cgcggctcgc cctggtgatc gcgggcggcc tggtggtcgt ttcgctgcag
     1201 ggcgtgtttg ccgtgatcgc ggctgcgatg atcgccttcg gcgccctgac gatgtggatc
     1261 gtcgccagcg cccggtggaa atgacatgaa agagtcgatc tccgaaagac tggccgacgc
     1321 gctcgcccgc gcgggtgagc cgccgcgcga gatcgtcgag aagctgttga tcgacgtcgc
     1381 ggggctttgc gtggcggcgc gcaataccga ctacgtgcac gccgcgctcg ccggctggga
     1441 agcctcggga aagtgcaccg cgatcggcca tgcgcgcgca ctcgactcgg ccggcgcggc
     1501 gttcgtgaat ggcaccgcgg cgcatggcga agacttcgac gacaccttcg aaggcggacc
     1561 ggtgcatgcc ggcgcggtca tcgtgcccgc cgttctcgcg ctctgcgagc gcgaaaaaat
     1621 ccccgggcaa atggctctgc gcggcatcgc ggtcggggtc gagctcacct gccggggcag
     1681 cacggtggcg ccgaagctga tccacaaggc cggtttccac ccgaccgcgg tgctcggggc
     1741 gatgggggcc gcggcggccg caggaacggt gttgaatctg accaagcccg aattcgtaaa
     1801 cgcgctgggt atcgccggct cgatggcctc ggggatcatc gagtacctcg cggaaggcac
     1861 ctggaccaag cgcatgcatc cgggctgggc ggcgcagtcg ggcatccgcg cggccgatct
     1921 cgcgcgcaat ggcttcgtcg ggccgcgcac cgtcttcgaa ggcgagcacg gcttctttca
     1981 cgggttcgcg cacaccacga gcggcgcgtg ggacaagctg gtcgatggct tcggcaggcg
     2041 ctgggtcacg gagacggtgg cgttcaagct ttatccctgc ggaaccatga cgcatccgta
     2101 catcgactgt gcgcgccgtc ttgcgaaaaa agtgaaggtc gaggagatcg aggaaatcgt
     2161 gtgcgaggcg gccgaaggga cactgcatcg cctgtgggag ccgctggcag ccaagcagcg
     2221 tccgcccaac gcgtatgccg gcaaattcag caccccgtat tgcatcgccg ccgggttcgt
     2281 cctcggcaac gcgggcctgg acgcgtttac cgacgagcgc gtggccgacc cgcgcctgcg
     2341 cgcgctcgcg gccaacgtgc gctacgaggt cgacccggac aacccctacc cgaacgaata
     2401 caccgggcat gtgcgcgtca gaacgagaga cggatcggtg gtggaagaga ggcaacccca
     2461 tctgcgtggc ggcgcgcacg agccgctgtc gcgcgccgat gtcgaggaga agttcaaggg
     2521 aaactgcgcc tatggcggct ggagcgccgc gcaggcggac gcgtttcttg cctgggcgaa
     2581 gaacgctttc ggcgccgcgc cggtcgacct ctcggcgttc aggaaataaa gccgatcagg
     2641 gcgtcgagcg tcgcgtccgg cgcttccgcc atcagcgtgt ggccggaagg cgcgatctcc
     2701 acggttttcg ccccggcaat cttctcctgc agaagcttca tggctctcgg cggcgtcatc
     2761 acgtcgcgcc ggccggcgat gaaaaggacc gcgcatttca ccccggccgc acgctcggcg
     2821 ccggcgccgt agtcgttgca cgccttgagg tcgttgtaga gcacgcccgg ggcgaggcgc
     2881 tcgagccgcg cgagcgcatc gccgtacatc cacatgcccg ggttcgggtt tgccgcgagc
     2941 ggcacctgcg ccgcgtgccc ccagatggtc gacatgtcga acgcggcgta gtcatcgtgg
     3001 cgcgcggcct cgagaaacgg ctcggcgacc ttcatcgggt agccgacggc gacgaacgcg
     3061 atgcgctcgc actgcgccgg atggcgcgcg gcgaactcga gcgcgaccag cgagcccatc
     3121 gagtggccga cgatgctcgc cttcccgatc ttgagctgct tcagaactgc ggacagccag
     3181 tccgccatcg cctcgatcga cggcaacggc gggccttcgc tgcgtccgtg gccgggaagg
     3241 tcgacggcga gcacgttgcg gccgtggtag ccgaagtagc ggctctgcaa ggcaaacgag
     3301 gaatggtcga ggcccgcgcc atgcacgaac acgacgctcg gcttgcttgc gtcgagctcg
     3361 tgggccgccg tataggcgaa cgcccgctta ccgttgactt cgaaattcac ttcccggaag
     3421 cgcgcagggc gcggtcgagg tcgtcgatca ggtcctgcgg atcctccagg ccgatcgaaa
     3481 gccgcaccgt gccggccgtg atgccggctt tcttgaggtc ctcgtcgctc atgcggtagt
     3541 gcgtggtggt cgccgggtgg atgaccagcg acttcgcgtc gcccacgttg gcgaggtgcg
     3601 agaacacgcg tagcgactcg atgaagcgct tgcccgcggg ccggccgccc ttgatctcga
     3661 aggtgaacac tgcgccgcag ccgtgcggca gcagcttctt tgccagcgca tggtcggggt
     3721 gctcagggag ctcgggatag gtcactttct cgacggaacc atttttcgac agaaaagcga
     3781 cgaccttgcg ggtgttctcg acatgccggg gcatgcgcag gtgcagcgtc tcgatgccct
     3841 gcaggatctg gaacgcggtc atcggcgcca tgcacgcgcc gaagtcgcgc agcccctcgc
     3901 ggcgcgcccg cagcaggaac gcccgcgtgc ccgactcctc ctcgaagtcc atgccgtgga
     3961 agccggcata gggcgcggtg agcgtctcga acttgcccga ggcttcccag tcgaagcggc
     4021 cggagtcgac cagcacgccc gcgatcgcca cgccgtggcc gccgaggaat ttggtcgccg
     4081 agtgatagag gagatctgcg ccgaactcga acggcttcat gagcaccggg gtggtaaacg
     4141 tcgcatcgac cagcagcggc accttggcct cgtgggcaat agtcgacaac gagggaatgt
     4201 cgagcacgtc caggcctggg ttgcccagcg tttcgccgaa cagcaagcga gtattgcttt
     4261 tgatgcttct tttccagtcg tccaggttcc gtggattgac gaaagtcgtg tcgatgccga
     4321 aacgcttgag tgtgtacgcg agcaggttgt gcgagccgcc gtagagcgcg gtcgaggcga
     4381 cgatgtggcc gcccgagccc atcagcgtgg cgatggcgag gtgcagcgcc gcctggcccg
     4441 aggcggtggc gatcgatccc accccgccct cgagcgcggc catgcgctcc tccagcaccg
     4501 cgacggtcgg attggagatg cgcgaataca cgtggcccgc gcgctccatg ttgaagaggg
     4561 aggccgcgtg ctcggtatcg cggaagacgt acgacgtggt ctggtagatc ggcaccgcgc
     4621 ggctgcccgt cgccgggtcg ggcgactggc cggcgtgcaa tgccagggtg tctaaggcgg
     4681 ggtacttcgg tgctgccatg gaattcgggg acggagccct aattccggca acgcgcagct
     4741 gaaggaatta gggctccgtc cccgaattcc atcagtgcag atttttcctg gacgggcggg
     4801 tgtagctctc gaaggcgcgc tcgggttttt tctgctgcga cgccttttct ttccaatcgc
     4861 ggtcgaacag ctcgttgacc agcgtggtga cctgcgcgac ttcctccttg gcgaactggc
     4921 gcgcgagaat cgccgcgacg tcctcgatca tgcagcggcg ctgcgcctcg tggatcgcct
     4981 gcgcggtcgg gccgacgaag agcagcttcg attcctgctc gccgttctcg tggtagagcg
     5041 tgaggtcgat ttccaccgcc tcgtagcaat acgggccgag cgcctccagc gcggcttcga
     5101 gcgcgccgtg gaagctgcgg ccgatctcgc cggtccagca caccgcgagc agcagctcct
     5161 tctcgtcgaa cttcaggccc ggctcttcgg gctcgaggct cttcacgtcg gctaagccgt
     5221 cggcgtcgag gtactcgagc agcggcgcga acgcctgctc aagctggcgc cgcgaaacgc
     5281 cttcgagaat ggggatgtcg gcgtggaggt gaacttcgtg ccgcatgc
//
LOCUS       DATBSZ010000113         9862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1109133, whole genome shotgun sequence.
ACCESSION   DATBSZ010000113 DATBSZ010000000
VERSION     DATBSZ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109133"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VIG70_07405"
     CDS             complement(<1..1171)
                     /locus_tag="VIG70_07405"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY8250417.1"
                     /translation="MSARNVVVIGTQWGDEGKGKIVDWLTDRAQGVVRFQGGHNAGHT
                     LVIGGKKTVLHLIPSGILREKVDCFIGNGVVVSPQALVEEMDELASAGARGVVARLSI
                     SEACPLILPYHSALDQAREAAKGEKKIGTTGRGIGPAYEDKAARRAIRLQDLFDPKRF
                     AEKLRELLEFHNFVLKNYYRFGEIDYQKTLDETLALAPRLAPLIADVPRALYHANRAG
                     KNLLFEGAQGSLLDIDHGTYPFVTSSNCVAGAAAAGAGIGPMHLHYVLGITKAYSTRV
                     GGGPFPTELSDNVGEMLRQRGQEFGATTGRPRRTGWFDAAALKRSIQLNGVSGLCITK
                     LDVLDGGESVKICVGYKIDGELSEILPVGAEELERCIPQYEELPGWRESTVGVKHF"
     gene            complement(1269..2210)
                     /locus_tag="VIG70_07410"
                     /pseudo
     CDS             complement(1269..2210)
                     /locus_tag="VIG70_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448278.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
     gene            complement(2221..2412)
                     /locus_tag="VIG70_07415"
     CDS             complement(2221..2412)
                     /locus_tag="VIG70_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEY8250418.1"
                     /translation="MWTTTFLMALALMLIFEGVLPFIAPNLWRDTFRKITQMTDGQIR
                     FVGLSSMIVGLLLLLWART"
     gene            complement(2417..3301)
                     /gene="hflC"
                     /locus_tag="VIG70_07420"
     CDS             complement(2417..3301)
                     /gene="hflC"
                     /locus_tag="VIG70_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY8250419.1"
                     /translation="MRALGPIVAILAVVALLVSSTLFTVDQRQNAIVFQLGEVKEVVN
                     RSGLHFKWPLLQNVRHFDMRILTFDDAEPLRFLTQGNRPVLVDSFVKWRIADVRQYFI
                     SVQGDEFRAATRIKQTVAGVLRDEFGARTVHEVVSGERDQVMSRVRDKVDQDLKRIGV
                     EIIDVRLKRVDLPQDVSESVYRRMEAERKRIANELRSTGAAEAEKIRADADRQREVLL
                     AEAYRDAQNVRGQGDAKSAAIYAAAFNQNPEFYSFYRSMEAYRSTFRGRNDLMLLETN
                     SDFLRYFRDSLGKPAARK"
     gene            complement(3302..4492)
                     /gene="hflK"
                     /locus_tag="VIG70_07425"
     CDS             complement(3302..4492)
                     /gene="hflK"
                     /locus_tag="VIG70_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY8250420.1"
                     /translation="MSLNDPNWGRGSRGPQGPGGGNQGPPDLDELWRNFNRRLSELFG
                     RRRGGDEPPRPPSARGLGGGAGVLVALVLAVWLASGFYIVVEGTRGVVLTFGRYSQET
                     ASGLRWRMPWPIQSHEIVNLQQVRTLEVGYRNNVRTKVLKESLMLTDDENIVDLQFAV
                     QYLVKDARDFVFNVRRPDESAMQIAETAMREIIGKNRMDAILYETQVDVANRSRDLMQ
                     QIHDRYGTGITVSTVTIQNAQPPEQVQAAFDDAVKAGQDRERQKNEGQSYANDVIPRA
                     RGTAARLQQEAEGYRQRVIASAEGDASRFKQVLAEYAKAPAVTRERIYIETMQAVLTS
                     TSKIMMDYRGAGNLLYLPLDKLMQGAGAPGEGAAPRAAPAEPPAEAGPRSRETLRNRE
                     RGDR"
     gene            complement(4508..5653)
                     /gene="hflX"
                     /locus_tag="VIG70_07430"
     CDS             complement(4508..5653)
                     /gene="hflX"
                     /locus_tag="VIG70_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY8250421.1"
                     /translation="MLDRPDAAADPQAAIIVCLDFGDPHYAEGVEEVVRLVQSAGVLR
                     HQVLKGKRSRPDPRLYAGSGKVEEIGRAAEELNAMYVVFNHALSPAQERNVEKVIARR
                     VMDRAELILEIFAQRAQTSEGKLQVELAQLEHLSTRLVRGWTHLERQRGGLGKTGGPG
                     ETQLELDRRYIASRVKTLKSKMVQLQKQRSVQRRARARRDVLSVSLVGYTNAGKSTLF
                     NALARAKAYEADQLFATLDTTTRRVWIPEAGNVVISDTVGFIRDLPHGLVAAFRATLE
                     ETVHADLLLHVVDASSAVRDEQANAVDKVLAEIPAGEAPRLLVWNKIDAARLEPGVER
                     DQYGRISRVFVSARTGAGLELLRGAIAEFAKSRAAQRGASALPLHTH"
     gene            complement(5676..5912)
                     /gene="hfq"
                     /locus_tag="VIG70_07435"
     CDS             complement(5676..5912)
                     /gene="hfq"
                     /locus_tag="VIG70_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY8250422.1"
                     /translation="MSNKGQLLQDPFLNTLRKEHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLRNSVTQMVYKHAISTVVPSRPISLGPDKEPE"
     gene            complement(5981..7069)
                     /gene="aroC"
                     /locus_tag="VIG70_07440"
     CDS             complement(5981..7069)
                     /gene="aroC"
                     /locus_tag="VIG70_07440"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY8250423.1"
                     /translation="MSGNTFGTLYCVTSFGESHGPAYGGVVDGCPPGLELSSADIQRE
                     LDRRKPGSSRHVTQRRESDAVEILSGVYEGRTTGTAIGFLIRNEDQRSKDYSAIADKF
                     RPGHADYTYWQKYGIRDARGGGRASARETVVRVAAGAIAKKWLKERYGVVVRGYLAQL
                     GPIRIAFASWHEVEGNPFFAPNAKIVPQLEKFVDQLRESGDSCGARVNVVAEGVPVGW
                     GEPVYGRLDADIAWAMMSINAVKGVEIGAGFGAIEQKGSEHGDEMTPRGFVQNNNGGV
                     LGGISTGQDVTVSLALKPTSSIRLPRKTINTRGEATSIETTGRHDPCVGIRATPIAEA
                     MLACVLADHALRHRAQCADVTPPTRLTR"
     gene            complement(7077..8270)
                     /locus_tag="VIG70_07445"
     CDS             complement(7077..8270)
                     /locus_tag="VIG70_07445"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8250424.1"
                     /translation="MGVAAAAIGAAAWRYWPEQGFWNPCHAQLPPRLANHDLVRQAWD
                     GIDASQCWDSHSHLIGSGDSDSGIFVNPATDSLLNPGQYARRLFFLNAGCVHDAAGSV
                     DRAYVDRVRNLIDGMRPGVKLLLYAFERAYDERGEPDLPHTMFHIPNAYARDVAKRHP
                     ESFEWAASIHPYRKDALEALEQAKRDGARAVKWLPSAMGIDPASPQCDRFYRALNKLN
                     LPLIAHAGLERAVLGREAHDFSNPLRLRRALDAGVRVVVAHCASMGEDRDLDRGANGP
                     YVESFSLFVRIFEKHKNAYGDISAMTQINRAGPALARVVENDAWHARLLNGSDYPLPG
                     VMPIFSVDYLVSLKMIEAGAADVLREIRLHNPLLFDFVLKRILRSNGKALSTSVFQTR
                     QFFMR"
     gene            complement(8290..8724)
                     /locus_tag="VIG70_07450"
     CDS             complement(8290..8724)
                     /locus_tag="VIG70_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8250425.1"
                     /translation="MQVKEILRVKGSRLISIEPAGRAADAVATMAKENLGSLVVLDQG
                     RMMGMLTFHELLRALATRAGALGEVKVSEIMLRNPVTATPDMEVNDLRRTMIESGARY
                     LPVMQDGKLLGVISFRDVAKAVLEEQDFENKMLKGYIKNWPA"
     gene            8787..9470
                     /locus_tag="VIG70_07455"
     CDS             8787..9470
                     /locus_tag="VIG70_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8250426.1"
                     /translation="MEKVALVTGGAQGIGRATALLLAGRGYQVAVADVQRSELFSVRT
                     DVSREPSARACVRAVVKRFGRLDALVNNAGLTGPASGPIEKLPLKDWNRRLAVNLTGA
                     FLMAKHAVPHLRRARGAMVNIASTRALQSEPDTEAYAASKGGLVALTHALAMSLGPEV
                     RVNCISPGWISHKPVRKKDHAQHPVGRVGVAADVAELVAYLLSDAASFVTGQNFVIDG
                     GMTRKMIYV"
     gene            complement(9486..>9862)
                     /locus_tag="VIG70_07460"
     CDS             complement(9486..>9862)
                     /locus_tag="VIG70_07460"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEY8250427.1"
                     /translation="PYDVEAALGAAASAKAQAALVLSSGAFFVVRDKLIAAAHRHRLP
                     VVANPNYAEAGALVAFGASFSHMYARAAEYADRILKGAKPNEMPVEQPSQYELIVNLK
                     SARALGIKLPQPVLVRASRIIE"
BASE COUNT         1528 a         3321 c         3283 g         1730 t
ORIGIN      
        1 cgaagtgctt tacgccgacc gtgctctcgc gccagcccgg aagctcctcg tactgcggta
       61 tgcagcgctc gagctcctcg gcgccgaccg gcaggatctc cgacagctcg ccgtcgatct
      121 tgtagccgac gcagatcttc accgactcgc cgccgtcgag cacgtcgagc ttggtgatgc
      181 acaggccgga gacgccgttc agctggatcg agcgcttgag cgcagcggcg tcgaaccagc
      241 cggtgcgccg cgggcggccg gtggtggcgc cgaattcctg gccgcgctgg cgcagcatct
      301 cgccgacgtt gtcggacagc tcggtcggga acggcccgcc gccgacgcgc gtgctgtagg
      361 ccttggtgat gcccagcacg tagtgcaggt gcatcggccc gatgccggcg cccgccgcgg
      421 ccgcgccggc gacgcagttc gacgaagtca cgaacggata ggtgccgtgg tcgatgtcga
      481 gcagcgagcc ctgcgcgccc tcgaacagca ggttcttgcc ggcgcggttg gcgtggtaga
      541 gcgcgcgcgg cacgtcggcg atcagcggcg cgaggcgcgg cgcgagcgcc agcgtctcgt
      601 ccagggtttt ctgatagtcg atttccccga accgatagta gtttttcagg acgaagttgt
      661 ggaactcgag cagctcgcgc agcttctccg cgaagcgctt ggggtcgaac aggtcctgca
      721 gccggatcgc gcgccgcgcc gccttgtcct cgtaggccgg gccgatgccg cggccggtgg
      781 tgccgatctt cttctcgccc ttcgccgcct cgcgcgcctg gtcgagcgcg gagtggtacg
      841 gcaggatgag cgggcaggct tccgagatcg agagccgcgc caccacgccg cgcgccccgg
      901 ccgatgcgag ctcgtccatc tcctccacca gcgcctgggg cgagaccacc acgccgttgc
      961 cgatgaagca gtcgaccttc tcgcgcagga tgcccgacgg gatcaggtgc agcaccgtct
     1021 tcttgccgcc gatcaccagc gtgtggccgg cgttgtgccc gccctggaaa cgcaccacgc
     1081 cctgggcgcg gtcggtgagc cagtcgacga tcttgccctt gccttcgtcg ccccactgcg
     1141 tgccgatgac gacgacgttg cgcgcgctca tacgctcgcg gacagaatca gggacagacc
     1201 acgtttttgc ccgcctgcag gcccccggaa aacgtggtct gtccctggtt tcggtttcgt
     1261 cattgcatta ccccggcgag acttctcaga tcgatcgaaa aacccgtcgc gggtcttgca
     1321 cggccaaaag ctttgcccac ctcgtcgtaa cggccacctc gtgccaccgc acccgtaacg
     1381 ccatcgcagt aagcgtcaaa taccacgccc gaatggtagt ggtagccgcg cagctcggcg
     1441 agatcgaagc tggcgggaaa cttgcaggct ttggcgagct tgcgcagcgt ggcgagcgcg
     1501 cgcgtgattt cggggagctt cggaagcttt gcctccgcga cgtcgagcac ttcggcgttg
     1561 ccgtagagac ttggcagcag gaggagagca tctcgcgctt tcgccttcag agaacgactt
     1621 agatttttta tctccggtat gtccttgcgc tgcaaggcct cgaacagctc cgcctcgcgc
     1681 tccgctccca cctccgcctg gtgcgcgatc gcgcggaaca cggcgacgtg tccgatgtcg
     1741 atgcgcgccg cgcgcacgcc ggcgagctcg agcgcgcggc acaggagctc gaggatctcg
     1801 acgtccgcct ccacgccggc ggcgccgtac atctcggcgc cgatctgaag cggctcgcgc
     1861 gtggcgcccg ggctcaacgg ccgggtgtgc agcacgctgc cgcagtagca cagccgggtc
     1921 acgcctttgc ggttgaggag atgcgcgtcg atgcgcgcca cctgcggcgt gatgtcggcg
     1981 cgcacgccca tcatccgccc cgagagctgg tcgacgacct tgaaggtgcg caattccagg
     2041 tcgtgcccgg tgccggtgag gagcgagtcc atgtactcga ggagcggcgg catcacgagc
     2101 tcgtactttt tctcgaagaa cagatcgaga atcttgcgcc gcagattttc gatgcgcctg
     2161 gcttcggccg gaagaatgtc ctcgatgtat tccggcagaa cccacttcaa cgcatttctc
     2221 ctaggtgcgc gcccacagca gaagcagcag gccgacgatc atcgacgaca gcccgacgaa
     2281 gcggatctgg ccgtcggtca tctgcgtgat cttgcgaaag gtgtcgcgcc agaggttcgg
     2341 cgcgatgaac ggcagcacgc cctcgaagat cagcatcagg gcgagcgcca tgaggaacgt
     2401 cgtcgtccac atggcgttac ttgcgcgccg cgggcttgcc tagcgagtcc ctgaagtagc
     2461 gcaggaagtc ggagttggtc tcgagcagca tgaggtcgtt gcggccgcgg aacgtcgagc
     2521 ggtaggcctc catgctgcga tagaaggaat agaactcggg gttctggttg aacgccgcgg
     2581 cgtaaatcgc cgcggacttg gcatcgccct ggccgcgcac gttctgcgcg tcgcgatagg
     2641 cctccgccag cagcacctcg cgctgccggt cggcgtcggc gcggatcttc tccgcctcgg
     2701 ccgcgccggt ggagcgcagc tcgttggcga tgcgcttcct ctccgcctcc atgcggcggt
     2761 agaccgactc gctcacgtcc tgcggcaggt cgacgcgctt caggcgcacg tcgatgatct
     2821 cgacgccgat gcgcttcagg tcctggtcga ccttgtcgcg cacacggctc atcacctggt
     2881 cgcgctcgcc tgacaccacc tcgtgcaccg tgcgcgcgcc gaactcgtcg cgcagcacgc
     2941 cggcgacggt ctgcttgatg cgcgtcgcgg cgcggaactc gtcgccctgc acgctgatga
     3001 agtactggcg cacgtccgcg atacgccact tgacgaagga atcgaccagc accgggcggt
     3061 tgccctgggt gaggaagcgc agcggctcgg cgtcgtcgaa ggtgaggatg cgcatgtcga
     3121 agtggcgcac gttctgcagc agcggccact tgaagtgcag ccccgagcgg ttgaccactt
     3181 ccttcacctc gccgagctgg aacacgatcg cgttctggcg ctggtcgacg gtgaacagcg
     3241 tgctcgacac gagcagcgcg acgacggcga ggatcgcgac gatcgggccg agggctctca
     3301 tctagcgatc gccccgctcg cgattgcgca gcgtctcgcg cgagcgcggg ccggcctcgg
     3361 ccggcggctc ggcgggcgcg gcgcgcggcg ccgcgccttc gcccggcgcg ccggcgccct
     3421 gcatcagctt gtcgagcggc aggtagagca ggttccccgc gccgcggtag tccatcatga
     3481 tcttgctggt cgaagtaagg accgcctgca tggtctcgat gtagatgcgc tcgcgcgtca
     3541 ccgccggcgc cttggcatac tcggcgagca cctgcttgaa tcgcgaggcg tcgccctcgg
     3601 cgctggcgat cacgcgctgg cggtagcctt cggcctcctg ctgcagccgc gcggcggtgc
     3661 cgcgcgcgcg cgggatgacg tcgtttgcgt acgactggcc ctcgttcttc tgccgctcgc
     3721 ggtcctggcc ggccttcacc gcgtcgtcga acgccgcctg cacctgctcg ggcggctgcg
     3781 cgttctggat cgtgaccgtg ctcaccgtga tcccggtgcc gtagcggtcg tggatctgct
     3841 gcatcaggtc gcgcgagcgg ttggccacgt ccacctgcgt ctcgtacagg atcgcgtcca
     3901 tgcggttctt gccgatgatt tcccgcatcg cggtctcggc gatctgcatc gccgactcgt
     3961 cgggccggcg cacgttgaac acgaagtcgc gcgcgtcctt caccaggtac tgcaccgcga
     4021 actgcaggtc gacgatgttc tcgtcgtcgg tgagcatcag cgattctttg agcaccttgg
     4081 tgcgcacgtt gttgcggtag cccacttcca gcgtgcgcac ctgctgcaga ttgacgatct
     4141 cgtgcgactg gatcggccac ggcatgcgcc agcgaagccc cgaggcggtt tcctgcgagt
     4201 agcggccgaa ggtgagcacc acgccccgcg tgccttccac cacgatgtag aacccgctcg
     4261 ccagccacac cgcgagcacc agcgccacca gcacgccagc gccgccgccc aggccccgcg
     4321 cgctcggcgg ccgcggcggc tcgtcgccgc cgcggcggcg gccgaacagc tccgagagcc
     4381 ggcggttgaa gttgcgccac agctcgtcca ggtccggcgg gccctggttg ccgccaccag
     4441 ggccctgcgg gccgcgggag ccgcgtcccc agttggggtc gttcaaggac acttggtcaa
     4501 atgcgctcta gtgagtatgt agcgggaggg cgctggcgcc tcgttgtgct gcccggctct
     4561 ttgcgaattc ggcgatcgcg ccgcgcagca actcgagtcc ggctccggtg cgtgcgctga
     4621 cgaaaacgcg ggaaatccta ccatactggt cacgctccac tccgggctcc agcctcgccg
     4681 catcgatctt gttccagacc aggagccggg gcgcttcccc agcgggaatt tccgcgagca
     4741 ccttgtccac ggcgttggcc tgctcgtcgc gcacggccga agaggcgtcg accacgtgca
     4801 gcaacaggtc cgcgtgcacg gtttcttcga gcgtcgcgcg aaacgccgcc accaggccgt
     4861 gcggcaggtc gcggatgaag ccgacggtat cggaaatcac cacatttccc gcttcgggta
     4921 tccacacccg ccgcgtggtc gtatcgaggg tggcgaaaag ctggtccgct tcgtaggcct
     4981 tggcgcgcgc gagcgcgttg aacagcgtcg acttgccggc gttggtatag ccgacgagcg
     5041 agaccgacag cacgtcgcgc cgcgcgcggg cacgtctctg gacgcttctc tgcttttgca
     5101 gctgaaccat tttcgatttc agcgtcttta cccgcgacgc gatgtagcgc cgatccagct
     5161 cgagctgggt ctcgccgggg ccgccggtct tgccgaggcc gccgcgctgg cgctcgaggt
     5221 gggtccagcc gcgcaccagg cgcgtcgaca ggtgctcgag ctgcgcgagc tccacctgca
     5281 gcttgccctc gctcgtctgc gcgcgctgcg cgaagatctc gaggatcagc tcggcgcggt
     5341 ccatcacgcg gcgcgcgatc accttctcga cgttgcgctc ctgcgcaggc gacagcgcgt
     5401 ggttgaacac cacgtacatg gcgttcagct cctcggcggc gcggccgatc tcctccacct
     5461 tgccgctgcc ggcgtacagg cgcgggtcag gcctgcttct cttgcccttc aggacctgat
     5521 ggcgaagcac gccggcgctt tgcaccagac gcaccacttc ctcgacgcct tccgcgtaat
     5581 gcgggtcgcc gaagtcgagg caaacgatga tcgccgcctg ggggtcggcg gctgcgtcgg
     5641 gacggtctag caaataacga tggctgagac gcccgctact cgggttcctt gtcggggccg
     5701 aggctgatcg ggcgcgaggg gacgacggtc gagatggcgt gcttgtacac catctgggtg
     5761 acggagttgc gcagcagcac cacgtactgg tcgaacgatt cgatctggcc ctgcagcttg
     5821 atgccgttta ccaggtagat cgagaccggg acgtgctcct tgcgcagcgt gttgagaaac
     5881 gggtcttgta gcaactgccc cttgttgctc atgttcttct ctcttggaag ttttatcgcc
     5941 tggcttatgg ccatactcta attgatttta ctgggcttta ctaccgggtc agccgggtcg
     6001 gcggcgtcac gtcggcgcac tgggcgcggt ggcgaagggc atggtcggcg agcacgcagg
     6061 cgagcatcgc ttcggcgatc ggcgtggcgc ggatgccgac gcaggggtcg tggcgcccgg
     6121 tggtctcgat ggaggtcgcc tcgccgcggg tgttgattgt ctttcggggc agccgaatgc
     6181 tggaggtggg cttcaacgcg agcgagaccg tcacgtcctg gccggtggaa atgccgccga
     6241 gcacgccgcc gttgttgttc tgaacaaatc cccggggtgt catctcgtcg ccgtgctcgc
     6301 tgcccttctg ctcgatggcg ccgaatcccg cgccgatctc gacgcctttc accgcgttga
     6361 tcgacatcat cgcccaggcg atgtcggcgt cgagccggcc gtagaccggc tcgccccagc
     6421 ccaccggcac cccctcggcg accacgttca cgcgcgcgcc gcacgagtcg ccgctctcgc
     6481 gcagttggtc cacgaacttc tcgagctgcg gcacgatctt cgcgttgggc gcgaagaacg
     6541 gattgccttc cacttcgtgc cacgaagcaa aagcgatcct gatcgggccg agctgggcga
     6601 gatagccgcg caccacgacg ccgtagcgct ccttgagcca tttcttcgca atcgcgccgg
     6661 cggcgacgcg caccacggtc tcgcgcgccg aggcgcggcc gccgccgcgc gcgtcgcgga
     6721 tgccgtactt ctgccagtag gtgtagtcgg cgtggccggg acggaacttg tcggcgatcg
     6781 ccgagtagtc cttcgagcgc tggtcctcgt tgcggatgag aaagccgatc gccgtgcccg
     6841 tggtgcgtcc ttcgtagacg cccgagagaa tttccacggc gtcggactcg cgccgctgcg
     6901 tgacatggcg cgatgagccg ggctttcttc tatccaattc cctctgtatg tcagcggacg
     6961 aaagttcgag ccccggcgga cagccgtcga cgaccccgcc gtaggcgggt ccgtgcgact
     7021 cgccgaacga ggtcacgcag tacagcgtgc cgaaggtatt gccggacatg cagaaatcat
     7081 ctcatgaaaa actgccgggt ctggaacacg ctcgtggaaa gcgccttgcc gttcgaacgc
     7141 agaatgcgct tgagcacgaa gtcgaacagc agcgggttgt gcaggcggat ctcccgcagc
     7201 acgtcggccg cgcccgcctc gatcattttc agcgacacca ggtagtcgac cgagaagatc
     7261 ggcatcacgc ccggcagcgg atagtcggag ccgttcagca ggcgcgcgtg ccaggcgtcg
     7321 ttctcgacca cgcgcgcgag cgccgggccg gcgcggttga tctgcgtcat ggcggagata
     7381 tcgccatagg cattcttgtg tttctcgaaa attcgaacaa agagggaaaa actctcgacg
     7441 tagggcccgt tcgcgccgcg gtcgaggtcg cggtcctcgc ccatcgaagc gcaatgcgcc
     7501 accacgacgc gcacgccggc gtcgagcgcg cgccgcagcc gcagcgggtt gctgaagtcg
     7561 tgcgcctcgc ggccgagcac cgcgcgctcg aggccggcgt gggcaatcag gggtagattc
     7621 aatttgttca gagcccgata aaagcgatcg cattgcggcg aggcgggatc gatgcccatc
     7681 gccgaaggca gccacttcac cgcgcgtgcg ccgtcgcgct tcgcctgctc gagcgcctcg
     7741 agcgcatcct tgcggtaggg gtggatcgag gcggcccatt cgaaggactc gggatggcgc
     7801 ttcgcgacgt cgcgggcgta ggcgttcgga atatggaaca tggtgtgcgg caggtcgggc
     7861 tcgccgcgct cgtcgtacgc gcgctcgaat gcgtagagga ggagcttcac gccgggcctc
     7921 atgccgtcga tcagattgcg cacccggtcg acgtaggcgc ggtccacgct gccggccgcg
     7981 tcatgcacgc agccggcgtt caggaagaat agccgccgcg cgtactgtcc cgggttgagc
     8041 aggctgtcgg tcgcggggtt gacgaagata ccgctatcgg agtcgcccga cccgatgagg
     8101 tgcgagtggc tgtcccagca ttgggaggcg tcgattccgt cccaggcctg gcgcacgagg
     8161 tcgtggttcg cgagccgcgg cggcagctgc gcgtggcagg gattccagaa gccctgctcg
     8221 ggccaatagc gccaggcagc cgcgccgatt gcggcggctg cgacgcccaa tcctcccaga
     8281 agaaacgtcc tacgcaggcc agttctttat gtagcccttg agcatcttgt tctcgaagtc
     8341 ctgctcttcg agcacggcct tcgcaacgtc gcggaacgag atgacgccga gcagcttgcc
     8401 gtcctgcatc accggcaggt agcgcgcgcc gctctcgatc atggtgcggc gcaggtcgtt
     8461 cacttccatg tcgggcgtcg cggtcaccgg gttgcgcagc atgatctcgg acaccttgac
     8521 ctcgccgagc gcaccggcgc gcgtggcgag cgcgcgcagc aactcgtgga aggtcagcat
     8581 gcccatcatg cggccctgat ccagcaccac cagggagccg aggttctctt tcgccatggt
     8641 ggccaccgcg tcggcggcgc ggcccgccgg ctcgatgctg atcaagcggc tgcccttgac
     8701 gcgcaagatt tccttcactt gcatgggcgt gacctcctca agggaagtct agtccaaacg
     8761 ccgccgccgc ggatagcatg ggagctgtgg aaaaagttgc cctcgtgacc ggcggtgcgc
     8821 agggcatcgg ccgcgcgact gccctgctgc tcgccggccg cggctaccag gtcgccgtcg
     8881 ccgacgtcca gaggtcggag cttttctccg tcaggaccga tgtctcgcgc gagccctcgg
     8941 cccgcgcctg cgtccgcgcc gtggtcaagc gcttcggccg gctggacgca ctggtaaata
     9001 atgccgggct cacgggcccc gcttccggcc ccattgaaaa acttccactg aaggactgga
     9061 accgacgcct cgccgtcaac ctcaccggcg cgttcctgat ggcgaaacac gcggtgccgc
     9121 acctgcggcg cgcgcgcggc gcgatggtga acatcgcatc cacgcgcgcc ctgcagtcgg
     9181 aacccgacac cgaagcctac gccgcgagca aaggcggcct ggtcgccctc acccacgcgc
     9241 tcgcgatgag cctcggcccc gaggtgcgcg tcaactgcat cagtcccggc tggatcagcc
     9301 acaagccggt acggaagaag gaccacgcgc agcacccggt cgggcgcgtc ggcgtcgccg
     9361 cggacgtggc cgagctcgtc gcctacctgc tctccgacgc cgccagcttc gtcaccgggc
     9421 agaacttcgt catcgacggg ggcatgacgc gaaagatgat ttacgtctga ggagctgtgg
     9481 ttcctctact cgataatccg gctcgcgcgc accagcacgg gctgcggaag cttgatgccc
     9541 agcgcccggg ccgatttcag gttgacgatc agctcgtact gcgagggctg ctcgaccggc
     9601 atttcgttcg gttttgcgcc cttcaggatg cgatccgcgt attccgcggc gcgcgcatac
     9661 atgtgagaga agctggcgcc aaaggccacg agcgcgcccg cttccgcata gttcgggttt
     9721 gcgactacgg gcaggcgatg tcggtgcgcc gcggcgatca atttgtcgcg cacgacaaag
     9781 aacgctcctg aggagagcac cagcgcggcc tgcgccttcg ccgatgcggc cgcgccaagg
     9841 gcggcctcga cgtcatacgg gc
//
LOCUS       DATBSZ010000114         3962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1109142, whole genome shotgun sequence.
ACCESSION   DATBSZ010000114 DATBSZ010000000
VERSION     DATBSZ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109142"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VIG70_07465"
     CDS             <1..190
                     /locus_tag="VIG70_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="depolymerase"
                     /protein_id="HEY8250428.1"
                     /translation="NRWADTNRLIVLYPQAIARYWWVYNPRGCWDWWGYTGSRYATKE
                     GAQIRAVMGMVERLGAPR"
     gene            211..810
                     /locus_tag="VIG70_07470"
     CDS             211..810
                     /locus_tag="VIG70_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY8250429.1"
                     /translation="MKTASMLFAAALVLSLSGCGYNTLQSTDEQIKSAWSEVLNQYQR
                     RADLVPNLVATVQGFANQEKEVLTRVTEARARVGAIQATPELVENPEAFAKFQAAQGE
                     LSGALSRLLVVAENYPQLKSDANFRDLQAQLEGTENRIAVARNRYIKAVQEYNVTVRS
                     FPSNLTAMAFGYKTKANFAVENEREIAKPPKVDFGQKAK"
     gene            807..1646
                     /locus_tag="VIG70_07475"
     CDS             807..1646
                     /locus_tag="VIG70_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HEY8250430.1"
                     /translation="MKFFLLALGLAWAQVAVPPLTARVTDLTGTLTREQQAGLERMLQ
                     EFEARKGTQLAVLLVKTTAPEAIEQYALRVAEQWKLGRKQVDDGAILVVAKDDRALRI
                     EVGYGLEGVLNDATASRIIREIIVPRFREGDFYGGINAGLDRMLRLIDGEPLPEPARS
                     APAAGAGGAMQMLPVLLVVALIGGAILRAALGRFLGAAVTGGAVGFLAWMLAGVIAVA
                     LLAAVLAFFFTLAGGFGGRRPYGGMGLPRSGGWGGGSWGGGGGGFRGGGGGFGGGGAS
                     GRW"
     gene            1647..2144
                     /locus_tag="VIG70_07480"
     CDS             1647..2144
                     /locus_tag="VIG70_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY8250431.1"
                     /translation="MDLRRIARHLAVLPGAVKRAFPAAAMAAIERAIAQSETQHSGEV
                     RFAAEPALDTAALLAGQSARERAIEVFSLLRLWDTDERNGVLIYLLLAERDIEIVADR
                     GVSAKVGAAEWEAICREMEAALRRGEYGEAVVRGIEAASRLLARHFPRGAGDRNELPD
                     RPVTL"
     gene            2148..2591
                     /locus_tag="VIG70_07485"
     CDS             2148..2591
                     /locus_tag="VIG70_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HEY8250432.1"
                     /translation="MEPSLNRTAWSATLHCLTGCAIGEVLGMVIGTALGWSNGPTIAL
                     AIVLAFFFGYALTLRPLLSAVSFAAAMRLALAADTASITVMEIVDNAIMLVIPGAMGA
                     GLASLLFWASLAISLAIAAVAAFPVNRWLIARGRGHAVVHAHHHH"
     gene            2591..3928
                     /locus_tag="VIG70_07490"
     CDS             2591..3928
                     /locus_tag="VIG70_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEY8250433.1"
                     /translation="MDRRAFLASSGALALAPRVSFSQKRIDKLRLSVARQSLVGAAHP
                     ATEVWAYNGSVPGPELRFRQGERLRIEVENALGAGTTVHWHGVRLPNAMDGVPGVTQP
                     PIAAKGGRFTYEFDLPDAGTYWYHPHLGTPEQVARGLYGALIVEEREPPAADRDVVWV
                     LSDWELDREARIDEEFANPMAASHDGRIGNTVTLNGAVPDSFGVRAGERIRLRLINAA
                     SARIYGLAFEGHAPTLIAFDGQPVAPHPAARVVLGPGMRADLILDCTAQPGSRHRVVD
                     GFYRRNAYELIKLQYRDEAPLRRSFPPVPVLGMNPVARPELGTAARHRIVFGGGAMGA
                     MPRQAEHKGMFWTVNGKPVREHHHHEPLLTLKMKQSYVLELVNDTSWYHPIHLHGHVF
                     RILPGDRWGDTVLLEPKSGAEIAFVADNPGDWMLHCHVLEHQASGMMALVRVA"
BASE COUNT          616 a         1329 c         1397 g          620 t
ORIGIN      
        1 caaccgctgg gcggacacga accggctgat cgtgctctac ccgcaggcga tcgcgcgcta
       61 ctggtgggtc tacaaccccc gggggtgctg ggactggtgg ggctataccg gctcgcgcta
      121 tgccaccaag gagggcgcgc agatccgggc ggtgatggga atggtggagc ggctgggtgc
      181 gccgcggtag gctctggcga aggagatctc atgaagactg catcgatgct tttcgcggcg
      241 gcgctcgtgc tgtcgctttc gggctgcggc tacaacacgc ttcagtccac cgacgagcag
      301 atcaagtcgg cctggtccga ggtcctcaac cagtaccagc gccgcgccga cctggtgccg
      361 aacctggtcg cgacggtgca gggcttcgcc aaccaggaaa aagaagtcct gacgcgcgtc
      421 accgaggcgc gcgcccgcgt cggcgcaatc caggcgacgc cagagctggt cgagaacccc
      481 gaggcgttcg cgaagttcca ggcggcgcag ggcgagctct ccggcgcgct gtcgcgcctg
      541 ctggtcgtcg ccgagaatta cccgcagctc aagtccgatg cgaacttccg cgacctgcag
      601 gcccagctcg agggcacgga gaaccgcatc gcggtggcgc gcaaccgcta catcaaggcg
      661 gtgcaggagt acaacgtcac cgtgcgctcg ttcccgtcga acctgacggc gatggcgttc
      721 ggctacaaga ccaaggcgaa tttcgcggtc gagaacgaac gcgagatcgc caagccgccg
      781 aaggtggact tcggtcagaa agcaaaatga aattttttct gcttgcgctc ggcctcgcct
      841 gggcgcaggt cgcggttccg ccgctcacgg cgcgcgtcac cgacctcacg ggaacgctca
      901 cgcgcgagca gcaagcagga ctcgagcgca tgctgcagga gttcgaggcg aggaagggaa
      961 cgcagcttgc cgtgctcctc gtgaagacca ccgcgcccga ggcgatcgag caatacgcgc
     1021 tgcgcgtcgc cgagcaatgg aagctcggcc ggaagcaagt cgacgacggt gccatcctcg
     1081 tcgtggcgaa ggacgaccgc gcgctgcgca tcgaggtggg atacgggctc gaaggcgtgc
     1141 tgaacgacgc caccgcgagc cgcatcatcc gcgaaatcat cgtgccgcgc ttccgcgagg
     1201 gcgacttcta cggcggcatc aacgccggcc tcgaccgcat gttgcggctc atcgacggcg
     1261 agccgctgcc cgagccggcg agaagcgcgc ctgctgcagg ggccggcggc gcgatgcaaa
     1321 tgctccccgt gctcctcgtg gtcgcgctca tcggcggcgc gatcctgcgc gcggcgcttg
     1381 gcaggttcct cggcgcggcc gtcaccggcg gcgcggtcgg atttcttgcc tggatgctcg
     1441 ccggggtcat cgcggtcgcg cttctggccg ctgtgctcgc ctttttcttc acccttgcgg
     1501 gaggtttcgg cgggcgacgc ccctatgggg gaatggggct gccccggtcg ggcgggtggg
     1561 gcggcgggag ttgggggggc ggcgggggcg ggttccgcgg cggcggcggc ggcttcggcg
     1621 gcggcggcgc atccgggcgg tggtagatgg acctccgccg catagcgcgc catctggccg
     1681 tccttcccgg ggcagtgaag cgggcttttc ccgccgccgc catggcggcc atcgagcggg
     1741 cgatcgcgca gagcgagacc cagcacagcg gcgaagtgcg cttcgccgcc gagccggcgc
     1801 tcgataccgc agcgctcctc gccggccagt cggcgcgcga gcgcgcgatc gaagtgtttt
     1861 cgctcctgcg actgtgggac accgatgaga ggaacggcgt gctgatctac ctcctcctcg
     1921 ccgagcgcga catcgagatc gtggccgacc gcggcgtgag cgccaaggtg ggcgccgcgg
     1981 aatgggaagc gatctgccgg gaaatggaag ccgcgctcag gcgaggcgaa tacggcgagg
     2041 ccgtcgtgcg cggcatcgag gcggcgtcgc gactcctggc gcgccacttc ccgcgcggcg
     2101 ccggcgaccg caacgagctc ccggaccggc cggtgacgct ctagcgcatg gaaccaagtc
     2161 tcaaccgcac tgcatggtcg gccacgcttc actgcctcac cggttgcgcc atcggcgaag
     2221 tgctcggcat ggtgatcggc acggccttgg gctggagcaa tggcccgacg atcgccttgg
     2281 cgatcgtgct ggcttttttc ttcggctacg ccctgaccct gcggccgctc ctgtcggcag
     2341 tgtcattcgc ggcggccatg cggctcgcgc ttgcggctga taccgcgtcg atcacggtga
     2401 tggaaatcgt cgacaacgcc atcatgttgg tgatcccggg agccatgggc gcggggcttg
     2461 cgagcctgct gttctgggcg agccttgcga tctcgcttgc aatcgcggcc gttgctgcct
     2521 tcccggtcaa tcgctggctg atcgcccgtg gccgcggcca cgcggtggtc cacgcacatc
     2581 accatcattg atggatcgcc gggcgttcct cgcaagcagt ggagcgctgg cgctggcgcc
     2641 ccgggtttcg ttttcccaga agaggatcga caagcttcgc ctctcggtcg cgcggcaatc
     2701 tctcgtcggc gccgcccatc cagccaccga ggtctgggcc tacaacggaa gcgttccggg
     2761 cccggagctg cgcttcaggc agggcgagcg gctgcgcatc gaggtcgaaa acgcgctcgg
     2821 ggccggcacg acggtgcact ggcatggcgt gcggctgccg aacgcgatgg acggtgtgcc
     2881 gggcgtcacg cagccgccga tcgcggcgaa gggcgggcgc ttcacatacg agttcgatct
     2941 tccggacgcg ggcacctact ggtaccaccc gcatctcggc accccggagc aggtggcgcg
     3001 cggcctctac ggcgcgctga tcgtcgagga gcgcgagccg ccggcggccg accgcgacgt
     3061 cgtctgggtg ctatccgact gggagctcga ccgggaggcg cgcatcgacg aggagttcgc
     3121 caacccgatg gccgcaagcc atgacgggcg catcggcaac accgttacgc tgaacggcgc
     3181 ggtgcccgac agcttcgggg tacgcgcggg cgagcgcatc cggctgcggc tcatcaatgc
     3241 ggcctccgcc cggatctacg ggctcgcgtt cgaaggacac gcgccgaccc tgatcgcatt
     3301 cgacggccag cccgtcgcgc cgcaccccgc cgcgcgcgtg gttctcgggc cgggcatgcg
     3361 cgccgacctg atacttgatt gcacggcgca gccgggaagc cggcaccgcg tcgtcgacgg
     3421 cttctaccgg cgcaacgcgt acgagctgat caagctccag tatcgggacg aggcgccgct
     3481 gcggcgatcg tttccgccgg tcccggtact agggatgaat cccgtggcgc gacccgagct
     3541 cggaaccgcg gcccgccacc gcatcgtgtt cggcggcggc gcgatgggcg cgatgccgcg
     3601 gcaggccgag cacaaaggca tgttctggac ggtgaacggc aagccggtgc gggaacacca
     3661 ccaccatgag cctttgctga ccctcaagat gaagcagtcc tatgtgcttg agctggtcaa
     3721 cgacacgtcc tggtaccacc cgatccacct gcacggccat gtgttccgca tcctccccgg
     3781 ggatcgatgg ggcgacacgg tgctgctcga gccgaaaagc ggcgccgaga tcgcgttcgt
     3841 cgccgacaac cccggcgact ggatgctcca ctgccacgtc ctcgagcacc aggcgagtgg
     3901 aatgatggcc ctggtacgcg tcgcatgatc gatccggtgc agcgctacgc actgctcgcg
     3961 ct
//
LOCUS       DATBSZ010000115         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1313904, whole genome shotgun sequence.
ACCESSION   DATBSZ010000115 DATBSZ010000000
VERSION     DATBSZ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313904"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VIG70_07495"
     CDS             complement(<1..570)
                     /locus_tag="VIG70_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8250434.1"
                     /translation="MNLFDLKGKTALVTGGNGGIGLGMAQGLAAAGAKVAIAGRDKAK
                     NAAALKTLEGALAVEADLKDEKACRAMVEDAARRLGRLDILVNNAGTNVRKRPEEYSL
                     EEWKFVLDTNLTSAFVASQAAYSHMKRAGGGKIVNIGSMMSLFGAAFVAPYGASKGGI
                     VQLTRALACAWARDNIQVNAVLPGWIDTAL"
     gene            complement(567..1532)
                     /locus_tag="VIG70_07500"
     CDS             complement(567..1532)
                     /locus_tag="VIG70_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250435.1"
                     /translation="MPERASDWVTVKGGNEFPLAEHTKPHHGYPIADWEKVRAWVNGL
                     EPEARRAQAWTACERAWLLHFRDALGAGFRLDESESAAVVSSLEPNVARATLEFMART
                     VRRIGAVLQGIVQLAPWGKDILVVFDDEESYYRYVSYYYPETGEFAFSGGMHIDSGCS
                     HFVTVKKDLRVLEATIVHEMTHACLEHLPLPAWLNEGLAVNTEQRLAGAPPLALGASD
                     RLHDKLRRFWSVARIQEFWSGVAFHLADSQTFSYELARILVEQLAKDWPPFAQFVLRA
                     DRADAGAAAARECLGLDLGTLVTTLLERETPKSWSPDPAKWEKTE"
     gene            1589..>2208
                     /locus_tag="VIG70_07505"
     CDS             1589..>2208
                     /locus_tag="VIG70_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY8250436.1"
                     /translation="MNRIDAVRRFNRFYTRRIGALQQGYMGSAFPLPQARVLYEIGQR
                     EACTASELGAELDLDLGYLSRLLAGLRRQGMVQGEAAKEDARRVRLSLTARGRKAHQQ
                     LDTRSRDLVAGMLGKLGAPDQARLVGALQAVESVLDKKERQQIALRPHRPGDMGWVVH
                     AHGRLYFEERGWGERFEALVAGIAKDFIEKFDPSRERCWIAEMDGEP"
BASE COUNT          335 a          752 c          763 g          358 t
ORIGIN      
        1 aagggcggtg tcgatccagc cggggagcac cgcgttcacc tggatgttgt cccgcgccca
       61 ggcgcaggcg agcgcgcgcg tgagctgcac gatgccgccc ttgctcgcgc cgtagggcgc
      121 gacgaacgcg gcgccgaaga gggacatcat cgagcctatg ttcacgatct tgccgccgcc
      181 ggcgcgcttc atgtgagagt aggcggcctg cgaggcgacg aacgcgctcg tcaggttcgt
      241 gtccagcacg aacttccatt cctcgaggct gtattcctcc gggcgcttgc gcacgttggt
      301 gccggcgttg ttcaccagga tgtcgagccg gccgaggcgc ctggcggcgt cctcgaccat
      361 cgcgcggcac gccttttcgt ccttgagatc ggcttcgact gcgagcgctc cctcgagtgt
      421 cttcaacgcg gcggcgttct tggccttgtc gcgcccggcg atcgccacct tcgcgccggc
      481 cgccgcgagc ccttgcgcca tgccgaggcc gatgccgccg ttgccgccgg tcacgagcgc
      541 ggtctttccc ttgaggtcga acaggttcat tccgtcttct cccatttggc aggatcgggc
      601 gaccaggact tcggggtttc tctctcgagc agcgtggtga ccagcgtgcc gagatcgagg
      661 cccaggcact cccgcgcggc agccgctccg gcatcggcgc ggtcggcgcg caggacgaat
      721 tgcgcgaacg gcggccagtc cttggcgagc tgctcgacca ggatgcgcgc cagctcgtag
      781 gaaaaggtct ggctgtcggc aaggtggaac gcgacgccgg accagaattc ctggatgcgc
      841 gccaccgacc agaagcgccg cagcttgtcg tgaagccggt cggacgcccc cagcgccaga
      901 ggcggcgcgc cggcgagccg ctgctcggtg ttgacggcga gcccttcgtt cagccaggcg
      961 ggcagcggca ggtgctcgag gcaagcgtgg gtcatctcgt gcacgatcgt ggcctcgaga
     1021 acccgcagat ccttcttcac ggtgacgaag tggctgcatc ccgagtcgat gtgcatgccg
     1081 ccgctgaacg cgaactcgcc tgtctcgggg tagtagtacg agacgtaacg gtaatagctt
     1141 tcctcgtcgt cgaagaccac caggatgtcc ttgccccagg gcgcgagctg cacgatcccc
     1201 tgcaatacgg cgccgatgcg ccgcaccgtg cgcgccatga actcgagcgt cgcgcgcgcc
     1261 acgttcggct cgagcgaaga cacgaccgcc gcgctctccg actcgtccag gcggaacccg
     1321 gcgccgagcg cgtcgcggaa gtgcagcagc cacgcgcgct cgcacgcggt ccaggcctgg
     1381 gcgcggcgcg cttcgggctc gagcccattc acccaggcgc gcaccttctc ccagtcggcg
     1441 atcgggtagc cgtggtgcgg tttcgtgtgc tcggcgagcg ggaattcgtt gccgcccttg
     1501 accgtgaccc agtcggatgc gcgctccggc atggggtcga ttatgtaata cttgacaaaa
     1561 gcaagtatct ggcgggacca tcgccggcat gaatcgcatc gacgcggtgc ggcgcttcaa
     1621 ccgcttctac acccggcgca tcggcgcgct gcagcagggc tacatgggca gcgcgtttcc
     1681 gctgccgcag gcgcgcgtgc tctacgagat cggccagcgc gaggcgtgca ccgcgagcga
     1741 gctcggcgcc gagctggatc tcgacctcgg ctacctcagc cggctgctcg ccggcctgcg
     1801 gcgccagggc atggtgcagg gcgaggcggc gaaggaagac gcgcgccgcg tacggctttc
     1861 cttgacggcc cggggccgca aagcccacca gcagctcgac acgcgctcgc gcgacctggt
     1921 cgcgggcatg ctcggcaagc tcggtgcgcc cgaccaggcg cgcctggtgg gggctctcca
     1981 ggccgtcgaa tcggtgctcg acaagaaaga acgacagcag attgcgctgc gcccgcaccg
     2041 ccccggcgat atgggatggg tggtgcacgc ccacggccgg ctctatttcg aggagcgcgg
     2101 ctggggcgag cgcttcgagg ccctggtcgc cggcatcgcg aaggacttca tcgaaaaatt
     2161 cgatccgtcc cgcgagcgct gctggatcgc cgagatggac ggcgagcc
//
LOCUS       DATBSZ010000116        23396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_836440, whole genome shotgun sequence.
ACCESSION   DATBSZ010000116 DATBSZ010000000
VERSION     DATBSZ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23396
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836440"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2245)
                     /locus_tag="VIG70_07510"
     CDS             complement(<1..2245)
                     /locus_tag="VIG70_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8250437.1"
                     /translation="MGATRQRRKSPVRPARMSALLGLQKVELFAGLDSYSLREIAAQC
                     KWTRCKRNEYVIRRDGTDRDVYFVIAGLVRVGAAAGRGRRIIFRDIPAGEVFGEHSAI
                     DGRTRFADVLAVRESLLASMSPEVFRAILANHASVRERLLRRLTGSVRELADRLLELG
                     AQPVQRRIWVELLRLARVAGVDANRACLDPAPTNTDIASRVGTSREQISRELSSLARQ
                     GVLERTGRTLVLTDVASLERLAGDPRLDAQAEAAMAGEEPRASAGILAPRQRRAILAA
                     EISDSVAMMERDEERTVERYRAFLAHATSQTIPMHAGRSMLRIPADGFVAEFPDAVQA
                     LRCAFELHAELARFNANLAAPALGMRVGMHVADVIVEAFNVLGDGVNVAARLAELANP
                     GETIVSAQVRDQLTSGVEASVEDLGEQRLRNRERAVRAFRAWPPAHSAVWTPSRAVQA
                     HGRPSVAVIPFQLRSEDPRFESIGDGLADDVIAALSRMADFFVISRLSTMAFRHAPLS
                     VRRIGELLGVQYVLSGSVQTAYPGALLMAELADARDGRIVWSERFQGDLADVFAMQGE
                     LARKVVQSVAPFVRSLELHRARITSFEQLDAYAITLRGVELMHRVSREDFMQARTAFE
                     TAIARDPVSPRPHAWLAKWHILRIAHGVSDDVAQDSAAATACAARALDCDPDDALALA
                     ADAHVSAWSRHDLDAAESRLAQALAANPNEPLAWLWRGITHAWRGRGDEAVECTDRAL
                     SLSPLDPM"
     gene            2317..2562
                     /locus_tag="VIG70_07515"
     CDS             2317..2562
                     /locus_tag="VIG70_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY8250438.1"
                     /translation="MDTLARIRQLAAKEFSINPDGLDPAAPLDTLGIDSLSFIEFMFK
                     VEEEFGVSVSDEDLKTIKTLADLERHVAAALTAAGKV"
     gene            2572..3807
                     /locus_tag="VIG70_07520"
     CDS             2572..3807
                     /locus_tag="VIG70_07520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY8250439.1"
                     /translation="MAARRVAVTGAGVISPLGTTLEEFQRSLAAGRSGIRRLAPALSE
                     RSGVQVGATLDWNPAPFFKEAEAANLDRVSQFALAAAAQAIAASGLRMADLNRDRIGV
                     SWGTGMGGAHTQEATYRQVYGDDEWRVRPLTVVMSMSNAASSNIAVRHGLRGPFASYS
                     TACSSSAMALGEAMRAIAAGRADAMIAGGSEALLTPGVLAAWQALRTLAPADPADAAA
                     SCKPFDKRRAGLVLGEGAAAFILEEESRARARGAPIYGLLTGYGNTCDAVHMSRPDRD
                     GQVRAMRDALAEAELPPDAIGYINAHGTATTVGDVVEAEAINTVFGPAAREVRVSSTK
                     SLHGHLLGGSGALEFAAALLAFEGILPPTGFLEQPDPACVLRHVARTAERVAPPPRAV
                     MSNSFAFGGSNVVLVAARA"
     gene            3863..4159
                     /locus_tag="VIG70_07525"
     CDS             3863..4159
                     /locus_tag="VIG70_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250440.1"
                     /translation="MQNRSKGRLGLKVIHRHRAGDSTFRRVSELVFIRGRPRAVLGWI
                     DAGGVRTPIYICELDPAKLKKAAAMAGTYYYDEVTVDPRYEPALPAGDEKHPER"
     gene            4272..4661
                     /locus_tag="VIG70_07530"
     CDS             4272..4661
                     /locus_tag="VIG70_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8250441.1"
                     /translation="MGATILVVDDEESARELLYLHLSSAGYRVLLAEDAITAGHYLLE
                     QRVDMLLADIEMPFMDGLDLVQAIRNDPAVSSMPVVFVTSHGHYEDRAKELGAVAYLR
                     KPVRGDQLLATVAKHVAPRSQASAASM"
     gene            4978..5301
                     /locus_tag="VIG70_07535"
     CDS             4978..5301
                     /locus_tag="VIG70_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250442.1"
                     /translation="MKWTEKKMKFPIKGVSVNDLKIAADDLRDGRWELVMQLALVSAV
                     FGLGWLAWPEGLMSSPFASWDLVSVVFGSVTVLLYALGLVMLYFVVLEPVRVYVRGMS
                     STNDG"
     gene            complement(5511..5586)
                     /locus_tag="VIG70_07540"
     tRNA            complement(5511..5586)
                     /locus_tag="VIG70_07540"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5551..5553),aa:Phe,seq:gaa)
     gene            5641..6552
                     /locus_tag="VIG70_07545"
     CDS             5641..6552
                     /locus_tag="VIG70_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8250443.1"
                     /translation="MAEGDRLEQTVPLVAPDGYVLGATLFEGAAGTTPRHAATICGGA
                     AIPARAYRHFAAWLAARGVPVLTYDNRGIGASRPPRMSGFRAAFEDWSDYDCAAAIAW
                     LRGHYPGSQLIGIGHSIGGFVLASARNAEMLDRLLFIATHTGYWGDYHPRWRWLLAIM
                     WHGVMPAITRLCGYFPGRALGLGADIPADFAGQWARRRTPAVRYEGERAEACLARCRR
                     LQGRALALTFTDDAFATAQGTRRVLEHLPGVTAEHRMIAPRDAGLASIGHFGFFRASA
                     GKSLWPAVGDWILQQGNPNEKGAEAPF"
     gene            complement(6596..8251)
                     /gene="groL"
                     /locus_tag="VIG70_07550"
     CDS             complement(6596..8251)
                     /gene="groL"
                     /locus_tag="VIG70_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481718.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY8250444.1"
                     /translation="MPAKEVRFHEGARAKMVHGLNILADAVKVTLGPKGRNVVLERSF
                     GAPTITKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIVRE
                     GMKFVASGMNPMDLKRGIDKAVIAVVEELKKISKPCTTSKEIAQVGAISANADFDIGK
                     IISDAMDKVGKEGVITVEDGKSLQNELDLVEGMQFDRGYLSPYFINNPEKQIAVLEDP
                     YILLHDKKISSIRELLPILEQVAKAGKPLLIIAEEVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLEDMAILAGGTVISEELGLKLENVTLKDLGRAKKIEAAKENTTIID
                     GAGDKKQIEGRVKQIRVQIDEATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVPGGGVAFIRAKQVLEGKLKGDNQDQDGGIKIVMRALEE
                     PMRMIVDNSGAEPSVVLNKVLENKGNYGFNAQTEEYGDLVQQGVIDPTKVVRFALQNA
                     ASVAGLMLTTDAMVAELVEEKKGGGGHSHGGMPGMGGMGDMDM"
     gene            complement(8294..8587)
                     /locus_tag="VIG70_07555"
     CDS             complement(8294..8587)
                     /locus_tag="VIG70_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY8250445.1"
                     /translation="MKIRPLHDRVIVKRIEEERKSAGGIVIPDTAAEKPDQGEIVAVG
                     KGKKDDQGKLIALDVKPGDRVLFGKYSGQTVKVKGDELLVMREEDIMGVIEGA"
     gene            complement(8776..10245)
                     /gene="ubiD"
                     /locus_tag="VIG70_07560"
     CDS             complement(8776..10245)
                     /gene="ubiD"
                     /locus_tag="VIG70_07560"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041322210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEY8250446.1"
                     /translation="MKYSDLREFIAQLEANGDLKRIKAEVSPRLEMTEICDRVLRAGG
                     PALLFERPAGHQIPVLANLFGAVRRVAAAMGVARGEENRSALREIGQMLAFLREPEPP
                     KGLRDLWEKFPVWKQLLNMPPKTVSTASSQEIILEGKDVDLARLPVQTCWPQDAGPLI
                     TWGLTVTRGPAKPRQNLGIYRQQVIGRNRVIMRWLAHRGGALDYRDHVAVSKEPFPVA
                     VALGADPATMLAAVAPIPDTLSEYQFAGLLRGARTELVKCLGNALQVPASAEIILEGV
                     LGEETAEEGPHGDHTGYYNEVERFPVMAVERITMRRSPIYHSTYTGKPPDEPAMLGVA
                     LNEVFVPLLQKQFPEIVDFYLPPEGCSYRLAVVSMRKQYPGHAKRVMFGIWSYLRQFM
                     YTKLIVVCDDDVDVRDWKEVIWALTTRVDAARDTTLVENTPIDYLDFASPVSGLGSKM
                     GIDATNKWPGETGRRWGRPIVMDDAVKRRVDRLWQDLGI"
     gene            complement(10242..10778)
                     /locus_tag="VIG70_07565"
     CDS             complement(10242..10778)
                     /locus_tag="VIG70_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250447.1"
                     /translation="MNEPVSAVSRFEFASGLLRGTQLTLHPNCLVHRSAHHVETLPLA
                     GMTAVRVAFQRDTRKLGWGIALVVIALVMLAVAGPLGRFASGAAGEMATAGAQGVPGA
                     LHSFFRLLEALASLLPVAALGCVIIGGVLCALGWMGSTTLLISLPGAERLYSTRGRDT
                     LLLDFAESVSDRLMSLRR"
     gene            complement(10775..11641)
                     /gene="ubiA"
                     /locus_tag="VIG70_07570"
     CDS             complement(10775..11641)
                     /gene="ubiA"
                     /locus_tag="VIG70_07570"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEY8250448.1"
                     /translation="MDLAFIAKRLDAYERLIRLDKPIGALLLLWPTLWAVWLASGGRP
                     RPDILIIFVLGTLLMRSSGCAINDYADRGFDPHVERTRRRPLAAGEILPREALAVAAV
                     LAAAAFVLVLFLNGFAIALSFVALAIAALYPFTKRFFAFPQAILGIAFGFGIPMAYAA
                     IQERLPLECWLLLAANVFYSFAYDTEYAMVDRDDDARIGIRTAALTLGRWDVAAVMAS
                     YASMVLILAILGAYLSLSWPYYAGLAAAAAMMLYHWRLIRDRSREGSFKAFMHNNWVG
                     AAIFAGIYLGMS"
     gene            complement(11677..13698)
                     /gene="recG"
                     /locus_tag="VIG70_07575"
     CDS             complement(11677..13698)
                     /gene="recG"
                     /locus_tag="VIG70_07575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY8250449.1"
                     /translation="MKGGLEKQLQKLGLRSRFDFVLHLPLRYEDETSLTPLGSVVPGT
                     PIHVEAKVSKAKVMYRPTRQLIVYTDGPVLHFFNFFPSQLRQLQQAAEQGLKVRAFGE
                     VRRGRLGVEMAHPRYRLVKEGEPLPQTLTPIYPTTEKLRQHELRTRVLESLDAETLDD
                     TLPQWLRQRLNLADFGASVRFLHRPPPGTDLGVLSNRSHPAWRRVKFDELLAQQLSMR
                     FAYRRRRSRQAPVLTVNGALLKGFLSTLPFSLTKAQMRAMNEVLRDLREPHPMQRLLQ
                     GDVGSGKTIVAAIACLAAVDSGAQAAVMAPTEILSEQHWRKFNEWLAPLGVRLGYLRG
                     GMAKKEKKQMLEGRVLIGTHALIQKDVEFSRLGLVVVDEQHRFGVEQRLALRKKAAST
                     GGPASRAIVPHQLMMSATPIPRTLEMTYFADLDVSTIDEMPPGRQPVRTRTFSAEKRP
                     EVLKRIREACAEGQQAYWVCPVIEESKEGDLQTALDAHASLTSELKGLRVGLLHGRLP
                     PGDKALVMDAFEKGAMQVLVCTTVIEVGVDVPNASLMVIESAERFGLAQLHQLRGRIG
                     RGQRESVCILLFGAKLSEAAGKRLGIVRDCSDGFEIARKDLELRGPGEFLGERQSGAP
                     LLRFADLERDQDLVGAAVHAAAELIDRDPAAARAHVARWLGSRRELAEA"
     gene            complement(13694..14071)
                     /locus_tag="VIG70_07580"
     CDS             complement(13694..14071)
                     /locus_tag="VIG70_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEY8250450.1"
                     /translation="MKTPIQTRDAPEAIGTYSQAVRAGDTVYLSGQIGLDPGSMQLAE
                     GIDGQTHRVFRNLAAVAAAAGLSLDQAVRMTVYLTDLAHFARVNEIMAQYVREPYPAR
                     AAVGVAGLPRGAIVEIDAILHAD"
     gene            14090..14413
                     /locus_tag="VIG70_07585"
     CDS             14090..14413
                     /locus_tag="VIG70_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8250451.1"
                     /translation="MISQLEKLLGTPRDGALLRYSLGLEHAKGGNHERAIEYLRDAVT
                     RDPLYSAAWKALGKSLSDSNLHADALEAYRQGIAAAKQKGDRQAEKEMTVFAKRLEKL
                     GKQPG"
     gene            complement(14393..14770)
                     /locus_tag="VIG70_07590"
     CDS             complement(14393..14770)
                     /locus_tag="VIG70_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8250452.1"
                     /translation="MSDERLRRIAIADGSPAFLTAAANHVADLPGYILAGTSNTAMQA
                     LTLVSSAGPDVLLLDLGMPPVRGLEMVKQVKAAPGAPAVVAMTLFHTPEAAAAAKRAG
                     ADALVGKESFIAGLRDALARLFP"
     gene            complement(14868..15512)
                     /locus_tag="VIG70_07595"
     CDS             complement(14868..15512)
                     /locus_tag="VIG70_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8250453.1"
                     /translation="MIRVLLADDHALVRAGIHSLLQGMREVQVVGEASSGEEALELAN
                     RAQPDVVLMDIAMKGITGLEAAARLRERHPEVRVVILSMHSGEEYVLQALRAGAVGYL
                     LKDAATGELELALRSVMRGESWLSPSVSRQVVEGYMHRTGGETAPEVLTARQREVLKL
                     VAGGKSTKEIAFLLNLSVKTVETHRAQIMERLGIRDVAGLVRYALRTGLVPPEG"
     gene            complement(15509..16645)
                     /locus_tag="VIG70_07600"
     CDS             complement(15509..16645)
                     /locus_tag="VIG70_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY8250454.1"
                     /translation="MNPLRQVAALLQRLVDKIDPHEGRYRMLFERSPLPMWVIDAESL
                     RFLAVNEAALHLYEYSREEFLAMTAFDIRPPEDQDSFSAFVRREPGDVRHGTFRHLKK
                     SGEPIDISGVGHLVAWRGRPAWLVQINDITERLRTQQTLERLNRELELSHERLRALSR
                     RLFEVQEEERRRLARDLHDDVGQALTALKIQLESVARAGGEVAARSRVDECVDTVQHT
                     LERVRHLSISLRPPQLDDLGLAAALRSHLDRQARVAGLQAHFETEDAPQELAPDTETA
                     CFRVAQEAINNVLRHARARNLWVRLFTAGGRLAISVRDDGRGFELDSVRERSANGSSL
                     GLIGMEERMALAGGSFELRSAPGQGTVLLATFPLGAAGKERAVG"
     gene            16735..17820
                     /locus_tag="VIG70_07605"
     CDS             16735..17820
                     /locus_tag="VIG70_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEY8250455.1"
                     /translation="MHPEGSAEAAGLRYVNDAMPGISRRRAGAVFRYHGPDGAGVRDP
                     AVLARIKALAVPPAWEKVWICPRDDGHLQATGRDARGRKQYRYHPRWREVRDETKYGR
                     MLSFAQVLSRIRRRVRRDLALSGLPRDKVLATVVHLLETTRMRVGNAEYARDNDSFGL
                     TTLRTRQVRVRGSTMQFRFRGKSGVWHEFALSDRRLASIVRRMRDLPGYELFQYVDEA
                     GETRAVDSADVNAYLKSIAGKEFTSKDFRTWAGTVLAAEALHEFGAEGLTKAIEQVAR
                     LLGNTKAVCRKCYIHPAVIDACLDGSLGQAMNGCSAEACVKRLIARRRRPRSLVPALQ
                     QSLRKLRRRGTQLAGRRGRIRATHPIP"
     gene            18089..18304
                     /locus_tag="VIG70_07610"
     CDS             18089..18304
                     /locus_tag="VIG70_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250456.1"
                     /translation="MSRCENALAFEKEKCLQDERAAATGSANRPAATGASAPNSGSPA
                     PSSTATPAGTMAPQNTAPNPAPAGTVR"
     gene            complement(18403..19200)
                     /locus_tag="VIG70_07615"
     CDS             complement(18403..19200)
                     /locus_tag="VIG70_07615"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEY8250457.1"
                     /translation="MLVHIRYTAATGEKLVNVTFGPNNNIRRRTADTEEVHEVEIRNG
                     RISSTPFSLDENGFVLVEQRTAVRNFFDKAELKLVYYPEVSELIRSVSGAARAVVFDH
                     TLRSGDEAEREARSIREPLPSAHNDYTEWSGPNRVRELMGDEAETLLKRRFAIIQVWR
                     AINQPIQCNPLAIADSKSVAPEDLLIAERRYPHRIGQTYRLKYSPKHRWFYFPQMRRD
                     EALVFKVFDSESDGRARFTPHASFDDPSTPLGAPPRQSIEARALAFF"
     gene            complement(19510..19585)
                     /locus_tag="VIG70_07620"
     tRNA            complement(19510..19585)
                     /locus_tag="VIG70_07620"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19550..19552),aa:Ala,seq:cgc)
     gene            19635..20384
                     /locus_tag="VIG70_07625"
     CDS             19635..20384
                     /locus_tag="VIG70_07625"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEY8250458.1"
                     /translation="MQISPPFGYKEVVPFLKTQKVRLLAPGEVPEFVQRGNAVPISHT
                     EFQPIARHYPIVFTTGDEGKTYAAVAVLGISAGENLFYADSRWSSGAYIPAYARRYPF
                     CMARVNLNKVEQKDRLICVEKSSLDEEGGEAMFDAAGQASDKWKGVERLLAEYEADLE
                     RMREMCALLGDYGLLEPFSMQATLKKEKGGGSMQMSGMHRVAEKNLENLNTAQLKNLM
                     RKGFLARIYLHLLSLENFARLLDRKGERSAA"
     gene            20463..20882
                     /locus_tag="VIG70_07630"
     CDS             20463..20882
                     /locus_tag="VIG70_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250459.1"
                     /translation="MSRNEGAMSYSDLDKARECHGALREIFIHHPGGWADKEALYRVE
                     KLAWQAMTLVKDAECQEKMGIVEHYAAALYSEAEHHRWRMTSLPGADFLRLQILRALV
                     AFHGRLFAIEAARRNDVDPNAALARAPALEQGSERTG"
     gene            20951..21256
                     /locus_tag="VIG70_07635"
     CDS             20951..21256
                     /locus_tag="VIG70_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250460.1"
                     /translation="MNERRKSRVGLKVIHRHRISDKVFRRVSELVMIDGQPKALLGWI
                     NLGEVRTPIYLCDLDPSKLHRSATSATTFYYDEMTVDPRFEALGPGLRDGDLPTSAV"
     gene            21369..21569
                     /locus_tag="VIG70_07640"
     CDS             21369..21569
                     /locus_tag="VIG70_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250461.1"
                     /translation="MKTLLAIAVTAALTGCGLETASTAATAASIKKQEVQEAQKTMHN
                     AQQRIDAATQQMHQSAESREPQ"
     gene            21660..22229
                     /locus_tag="VIG70_07645"
     CDS             21660..22229
                     /locus_tag="VIG70_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY8250462.1"
                     /translation="MKNHRWLIVVGLLAAVTSGPAAAQLYAGGSIGYSQFKDVCVLAN
                     VPCDDTDTAWRAFGGYQFNPYFALEVGFADLGATTGSGSVGSFSVEAKQAWDLSALFT
                     IPVTTRFSFLARIGAHRVRTTVDQQVTGVGPLHEAKTGSSWTYGAGAEYSLGKLGVRA
                     EWQRYDNIAGGTLVEDDVDVLSIGLLFRF"
     gene            complement(22254..22327)
                     /locus_tag="VIG70_07650"
     tRNA            complement(22254..22327)
                     /locus_tag="VIG70_07650"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22291..22293),aa:Ile,seq:cat)
     gene            complement(22341..22766)
                     /locus_tag="VIG70_07655"
     CDS             complement(22341..22766)
                     /locus_tag="VIG70_07655"
                     /inference="COORDINATES: protein motif:HMM:NF022841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3192 domain-containing protein"
                     /protein_id="HEY8250463.1"
                     /translation="MGIWRTSILAAGVLAGCSTLIIDSIEQLRENNKRNIAQLAIGTP
                     RAQVEKLMGDASAGGKMGDILFGRLQYLEVKNPQRVERVTGPDGAAYDVLFYYTDLKA
                     RDDKITDDELTPLVFRGHELAGIGYGFLARLAPKYKGLQ"
     gene            complement(22797..>23396)
                     /locus_tag="VIG70_07660"
     CDS             complement(22797..>23396)
                     /locus_tag="VIG70_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8250464.1"
                     /translation="DKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETI
                     NKMNRISRQILQETGQEPDPATLAQRMEMPEEKIRKILKISKEPISMETPIGDDDDSH
                     LGDFIEDTSTVAPSEAATYASLRDVTKEVLDTLTPREAKVLRMRFGIEMSTDHTLEEV
                     GKQFDVTRERIRQIEAKALRKLRHPSRSERLKSFLDQEG"
BASE COUNT         3842 a         7855 c         7735 g         3964 t
ORIGIN      
        1 tcatcgggtc gaggggcgag agcgaaagcg cgcgatcggt gcactcgacc gcctcgtcgc
       61 cgcgtccccg ccaggcgtgg gtaattccgc gccagagcca ggcgagcggc tcgttggggt
      121 tggccgccag cgcctgcgcg aggcggctct cggcggcatc gagatcgtgt ctcgaccacg
      181 ccgagacgtg cgcatccgcg gccagggcga gcgcatcgtc ggggtcgcag tcaagcgcgc
      241 gcgcggcgca cgcggttgcc gccgcgctgt cctgggcgac gtcgtcgctt acgccgtgcg
      301 cgatgcgcag aatgtgccac ttggcaagcc aggcgtgcgg ccgcggcgaa accggatcgc
      361 gcgcgatcgc ggtctcgaac gccgttcgcg cctgcatgaa gtcctcgcgc gacacgcgat
      421 gcatcagctc gacgccgcgc agcgtgatgg cgtaggcgtc gagctgctcg aagctggtga
      481 tgcgcgcgcg gtgcagctcg agggagcgca cgaacggcgc gacgctctgc accaccttgc
      541 gagcgagctc tccctgcatc gcgaagacgt cggcgagatc gccctgaaag cgctcgctcc
      601 acacgatgcg gccgtcgcgc gcgtcggcga gctcggccat cagcagcgcg cccgggtagg
      661 cggtctgcac gctcccggaa agcacgtatt gcacgccgag gagctcgccg atccgccgca
      721 cgcttagcgg cgcatgccgg aaagccatgg tcgacaggcg cgagatgacg aagaagtccg
      781 ccatgcgcga cagcgcggcg atgacgtcgt ccgccaggcc gtcgccgatc gactcgaagc
      841 gagggtcctc cgaccggagc tggaacggga tcaccgccac ggagggccgg ccgtgcgcct
      901 gcaccgcccg gctcggcgtc caaaccgcgc tatgcgccgg cggccatgcg cggaacgcgc
      961 gcaccgcgcg ctcgcggttg cgcaagcgct gctcgccaag gtcctccacc gaggcctcga
     1021 cgccgctcgt gagctggtcg cgtacctgcg ccgacacgat cgtctcgccc ggattggcga
     1081 gctcggcgag gcgcgccgcg acgttcacgc cgtcgcccag gacgttgaac gcctcgacga
     1141 tgacgtcggc gacgtgcatg ccgacgcgca tgccaagcgc gggcgcagcc aggttggcgt
     1201 tgaagcgcgc gagctcggcg tgcagctcga aggcgcagcg cagcgcctgg accgcgtcgg
     1261 gaaattcggc gacgaagccg tcggccggaa tccgcagcat gctgcgaccg gcgtgcatgg
     1321 ggatggtctg cgacgtggcg tgggcgagaa acgcgcggta gcgctcgacg gtgcgctcct
     1381 catcacgctc catcatggcc accgaatcgg agatctcggc ggccagaatg gcgcgccgct
     1441 ggcgcggcgc gaggatgccg gcggaggcac gcggctcctc gcccgccatc gccgcttccg
     1501 cctgcgcgtc gagacgcgga tctccggcca gccgctcgag cgacgccacg tcggtcagca
     1561 ccaaagtgcg gcccgtgcgc tccagcacgc cctgccgcgc caggctggaa agctcgcgcg
     1621 agatctgctc gcgcgaggtg ccgacgcggc tcgcgatgtc ggtgttcgtc ggcgcgggat
     1681 cgaggcaggc gcggttggcg tcgactcccg cgaccctggc gaggcgcagg agctcgaccc
     1741 agatgcggcg ctgcaccggc tgcgcgccga gctcgagcag gcggtccgcg agctcgcgca
     1801 cggatccggt gagccggcgc agcaggcgct cgcgcaccga ggcatggttc gcgaggatcg
     1861 cgcggaacac ttcgggcgac atcgaggcga gtagcgattc gcgcaccgcg agcacgtcgg
     1921 cgaagcgtgt gcgcccgtcg atcgccgaat gctcgccgaa cacctcgccc gccggaatgt
     1981 cgcggaagat gatgcgccgg ccgcgcccgg ccgccgcgcc gacgcgtacc aagccggcga
     2041 tcacgaaata gacgtcgcga tcggtgccgt cgcggcgaat gacgtactcg ttgcgcttgc
     2101 agcgcgtcca tttgcactgc gcggcgatct cgcgcaggct gtagctgtcc aggcccgcga
     2161 acagctccac cttctgcagg ccgagcagcg cagacatgcg cgccggcctg accgggctct
     2221 tgcggcgctg gcgggtggcg cccatgccgc ctactgcagc gaggtgacgc ggatggcgaa
     2281 catcgtttcc tccatcgcag actataattc atccggatgg acacgctcgc tcgcatccgg
     2341 cagttggcgg cgaaggaatt ctcgatcaat cccgacggcc tcgacccggc agcgccgctc
     2401 gatacgctcg gcatcgactc gctcagcttc atcgagttca tgttcaaggt cgaagaggag
     2461 ttcggggtca gcgtgtccga cgaggacctg aagacgatca agacgctcgc cgacctcgag
     2521 cgccacgtcg ccgccgcgct caccgccgcc ggcaaagtct gatcccgcgg catggccgct
     2581 cgccgcgtcg ccgtcaccgg agccggcgtg atctcgccgc tcggcaccac cctggaggag
     2641 tttcagcgct cgctcgccgc gggacgctcg ggcattcgcc ggctcgcgcc ggcgctgagc
     2701 gagcgctcgg gggtgcaggt gggcgcgacg ctcgactgga accccgcgcc gttcttcaag
     2761 gaagccgagg ccgcgaacct cgaccgcgta tcgcagtttg ccctcgccgc ggcagcgcag
     2821 gccattgcgg caagcggtct gcgaatggcc gacctgaacc gtgaccgcat cggcgtgtcc
     2881 tggggcaccg gaatgggcgg cgcgcacacg caggaggcga cctaccgcca ggtctacggc
     2941 gacgacgaat ggcgcgtgcg gccgctgacc gtcgtgatga gcatgagcaa cgccgccagc
     3001 tcgaatatcg ccgtgcgcca cgggctgcgc ggcccgttcg cgagctactc gacggcgtgc
     3061 tcgtcgtcgg cgatggcgct cggcgaagcg atgcgcgcca tcgccgcggg acgcgcggac
     3121 gcgatgatcg ccggcggctc cgaggcgctg ctcacgccgg gcgttctcgc cgcctggcag
     3181 gcgctgcgca cgctggcgcc cgccgacccg gccgacgccg cggcgagctg caagcctttc
     3241 gacaaacgcc gcgccggcct ggtcctcggc gaaggcgcgg ctgcgttcat tctcgaggag
     3301 gaatcgcgcg cgcgcgcccg cggcgcgcca atctacggcc tgctcaccgg gtatggcaac
     3361 acctgcgacg ccgtgcacat gtcgcggccc gatcgcgatg gacaagtgcg tgcgatgcgc
     3421 gatgcgctcg ccgaagccga gctgccgccc gatgccatcg gctacatcaa tgctcacggc
     3481 acggcgacaa cggtcggtga cgtggttgaa gccgaggcga tcaacacggt cttcgggccc
     3541 gcggcgcgcg aggtgcgcgt gagctcgacc aagtcgttgc acggccatct cctcggcggc
     3601 tcgggcgcgc tcgaattcgc cgccgcgctg ctcgccttcg agggcatcct gccgcccacg
     3661 ggttttctcg agcaacccga tcccgcctgt gtcctgcgcc acgtggcgcg caccgccgag
     3721 cgcgtcgcgc cgccgccgcg ggccgtaatg tccaattcgt tcgctttcgg cggctcgaac
     3781 gtcgtgctgg tcgctgcacg cgcgtaaacg cgtgtagttc tttcccctac cggcacggcg
     3841 cctgcatggg attgcgtact ccatgcaaaa tcgcagcaaa ggccgactcg gcctcaaggt
     3901 gatccatcgc caccgcgcgg gcgactccac ctttcgtcgt gtttccgagc tggtcttcat
     3961 ccgcggtcgg ccgagggccg tgctcggctg gatcgacgcc ggcggcgtgc gtaccccgat
     4021 ctacatctgc gagctcgatc cggccaagct gaagaaggcc gccgcgatgg ccggaacgta
     4081 ctactacgac gaggtcaccg tagatccccg ctacgagccg gcgctgccgg ccggggacga
     4141 aaagcacccc gagcgctagg cgccggcgac ttcgatccgg tgaagcgtta agaaatgttt
     4201 ggccggtccg cgactccgct cgaggccctt acagtgtcga gcggaagaac tcccgggacc
     4261 gatcccacct gatgggcgca acgattctgg tcgtggatga cgaggagagc gctcgcgagc
     4321 tgctctacct ccacctctcg agcgccggct atcgcgtgct gctcgccgaa gacgccatca
     4381 ccgccgggca ttacctgctg gagcagcgcg tggacatgct gctcgccgac atcgaaatgc
     4441 cgttcatgga cggcttggat ctggtgcagg cgatccgcaa cgaccctgcg gtctcctcaa
     4501 tgccggtggt cttcgtgact tcgcacgggc actacgagga ccgcgcgaag gagctgggag
     4561 cggtcgcgta cctgaggaag cccgtgcgcg gcgaccagct gctcgccacc gtggcgaagc
     4621 atgttgctcc gcgatctcag gcgagcgccg cgtcgatgtg acctgcgtca cgcgctcagg
     4681 ctgatgccct gaggaggagc ctgatccctg cgatcaggga taacccgata cgggtcaggc
     4741 tttggcgcga tgaaggcagg tggtgcgcga tctcaggcgt gtggaaggag tcgcctgatc
     4801 gactggttgc gcgacaagtg ggcttcgatc actacctcgt aaagccctgc gatccaaaga
     4861 cgctgctacg tctctaaggc ggggacagcg tcgtgaactt gtttgcgcgg gcgagaggaa
     4921 ttcaagaaac ccctacagac gcttgctttg aacggggaat atattcgaag ggccgttgtg
     4981 aagtggaccg aaaagaagat gaaatttccc atcaaagggg tgtcggtgaa cgatctgaaa
     5041 attgctgctg acgatctgcg cgatggtcgt tgggagttgg tgatgcagct tgctcttgtt
     5101 tcggcggttt tcggcctcgg ctggttggcg tggcctgaag gtctgatgtc ctctccgttt
     5161 gcatcgtggg atctcgtctc ggtcgtgttc ggaagcgtga cggtactgtt gtacgccttg
     5221 gggctggtga tgctctattt cgtggtgctc gagccggtcc gggtgtatgt ccgtgggatg
     5281 agctcaacga atgacggtta gctgcgttcc catggcctca tgagggcctt agggacgcag
     5341 ctggcacgct tgctggcctt cccggccttc tcgagtccat aagactcacg aactagccgc
     5401 agtccgctct gcggtcggcg tcattcgtct cgccgttcgt cgcggccact accgggcccg
     5461 cgccttagcg cgctacgctc gctccaactg tatggcctga gactgggatt tggtggccaa
     5521 gggcggaatc gaaccaccga cacaaggatt ttcagtcctc tgctctacca actgagctac
     5581 ttggccgccg ggaaatcgca acgggagcaa ctgcggaagg cgcaaattat agcaacgcag
     5641 gtggctgaag gagatcgcct ggaacagacc gtgcctctcg ttgcgccgga cggctacgtt
     5701 ctcggcgcga cgctgttcga aggcgcggca ggcaccacgc cgaggcacgc tgcgacgatc
     5761 tgcgggggcg ccgcaatccc ggcacgcgcg taccggcact ttgcagcctg gctcgccgca
     5821 cgcggcgtac ccgtcctgac ttacgacaac cgcggcatcg gcgcttcgcg gccgccgcgg
     5881 atgtccggct tccgcgcggc attcgaggac tggtcggact acgactgcgc cgcggccatc
     5941 gcgtggctac gcggccacta cccgggctca cagctgatcg gcatcggcca cagcatcggc
     6001 ggcttcgttc tggcgagcgc ccgcaacgcc gaaatgctcg accggctgct gttcatcgcg
     6061 acccacaccg ggtactgggg cgactatcac ccgcgctggc gctggctgct ggcgatcatg
     6121 tggcacggcg tgatgcccgc gatcacccgc ctgtgcggct acttcccggg ccgggcgctg
     6181 ggcctgggcg cggacattcc cgccgacttc gcggggcaat gggcgcggcg acggacgccg
     6241 gcggtgcgct acgagggcga gcgcgccgag gcatgcctgg cacgctgccg ccgcctgcag
     6301 ggccgtgccc tggcgctcac cttcaccgac gacgcatttg cgaccgcgca aggcacgcgg
     6361 cgcgtgctgg agcatcttcc cggagtgacc gcggagcatc gcatgatcgc gccgcgcgac
     6421 gccggcctcg ccagcatcgg gcacttcggt ttttttcgag cgagcgcggg taaatcgctc
     6481 tggcccgccg tcggcgactg gatccttcag cagggaaatc caaatgaaaa gggcgccgaa
     6541 gcgccctttt gaatgggatc cccgcctgcg cggggataac tctgcgtagg cggagttaca
     6601 tatccatgtc gcccatgcca cccatgccgg gcatgccacc gtgcgaatgg ccgccgccgc
     6661 ccttcttctc ctcgaccagc tcggccacca tggcgtcggt ggtgagcatc aggccggcaa
     6721 cggaggcggc gttctgcagc gcgaagcgca cgaccttggt cgggtcgatc acgccctgct
     6781 gcaccaggtc gccgtactcc tcggtctggg cgttgaagcc gtagttgccc ttgttctcga
     6841 gcaccttgtt cagcaccacc gagggctcgg cgccgctgtt gtcgacgatc atgcgcatcg
     6901 gctcttccag cgcgcgcatc acgatcttga tgccaccgtc ctggtcctga ttgtcaccct
     6961 tgagcttgcc ctcgagcacc tgcttggcgc ggatgaacgc cacgccgccg cccgggacga
     7021 cgccctcttc caccgcggcg cgggtcgcat gcagcgcatc ctcgacgcgg gccttcttct
     7081 ccttcatctc gacttcggtg gccgcgccca ctttgatgac ggcaacgccg ccggcgagct
     7141 tcgccacgcg ctcctgcagc ttctccttgt cgtaatcgct ggtcgcctcg tcgatctgga
     7201 cgcggatctg cttgacccgg ccctcgatct gcttcttgtc gccggcgccg tcgatgatgg
     7261 tggtgttctc cttggcggct tcgatttttt tcgcgcggcc gaggtccttc agcgtcacgt
     7321 tctcgagctt gagaccgagc tcctcggaaa tcacggtgcc gccggcaagg atcgccatgt
     7381 cttccagcat cgccttgcga cggtcgccga agccgggcgc cttgaccgcg acggtcttga
     7441 gaatgccgcg gatgttgttc accaccagcg tggcgagcgc ctcgccctcg acttcctcgg
     7501 cgatgatgag gagcggcttg cccgccttcg ccacctgctc gaggatcggc agcagctcgc
     7561 ggatcgacga gatcttcttg tcgtgcagca ggatgtacgg gtcttcgagc actgcgatct
     7621 gcttctcggg attgttgatg aagtacggcg agaggtagcc gcggtcgaac tgcatgccct
     7681 cgacgagatc gagctcgttc tgcagcgact tgccgtcctc gaccgtgatc acgccttcct
     7741 tgccgacctt gtccatggcg tcggagatga tcttgccgat gtcgaagtcg gcgttggccg
     7801 agatcgcgcc aacctgggcg atttccttgg aggtggtgca gggcttggag atcttcttca
     7861 gctcctcgac gacggcaatg actgctttgt cgatgccgcg cttcaggtcc atcgggttca
     7921 tgcccgaggc gacgaacttc atcccctcgc gcacgatcgc ctgcgcgagc acggtcgcgg
     7981 tcgtggtgcc gtcgccggca acgtcggagg ttttggacgc gacttccttc accatctgcg
     8041 cgcccatgtt ctcgaacttg tccttgagct cgatctcttt cgcgaccgag acgccgtcct
     8101 tggtgatggt cggtgcgccg aacgagcgct cgagcacgac gttgcggccc ttcggcccca
     8161 gcgtcacttt cacggcgtca gccaggatgt tcagtccatg gaccatcttg gcgcgggcgc
     8221 cttcgtggaa acgtacttct ttggcaggca ttgcgattct cctaattcag ttcaataagt
     8281 gcgttgacca ggcttatgcg ccttcgatga cgcccatgat gtcttcctcg cgcatcacga
     8341 ggagctcatc gcccttcacc ttcacggtct gcccggaata cttgccgaac agaacgcggt
     8401 cgccgggctt gacgtcgagc gcgatcagct tcccctgatc gtctttcttg cctttgccga
     8461 cagccacgat ctcgccctgg tcgggcttct cggcggcggt gtcggggatg acgatgcccc
     8521 cggcggactt gcgctcttct tcgatgcgct tgacgatgac gcggtcatgc agcggacgga
     8581 ttttcatgaa gacctccaat tgagaaactt cgatgtatta caatggcttg gattcaccaa
     8641 gccaaacacg tttgggtgtt agcactcatc tacagcgagt gctaataata gcggttgtgc
     8701 cccgcctttt caagagcttc cccgtcctgt gggcgcttat ttcttttggc gccactggcg
     8761 ccgagcttca gtggatcaga tcccgaggtc ctgccagagg cggtctacgc gacgcttcac
     8821 cgcgtcgtcc atgacgatcg gccggcccca gcgccggccg gtctcgccgg gccacttgtt
     8881 cgtcgcgtcg atgcccatct tggagccgag cccggacacg gggctcgcga agtcgaggta
     8941 gtcgatcggg gtgttttcga ccagcgtcgt gtcccgcgcg gcgtctaccc gtgtggtcag
     9001 cgcccagatc acttccttcc agtcgcgcac gtcgacgtcg tcgtcgcaca ccacgatgag
     9061 cttggtgtac atgaactgcc tgagatagct ccagatgccg aacatcacgc gcttggcgtg
     9121 gccgggatac tgcttcctca tgctcaccac ggcgaggcgg tacgagcagc cctcgggcgg
     9181 caggtagaag tccacgatct cgggaaactg cttttgcagc agaggcacaa aaacttcatt
     9241 cagcgccacg ccgagcatcg ccggctcgtc gggcggcttg ccggtgtagg tcgagtgata
     9301 gatcggcgag cggcgcatcg tgatccgctc caccgccatc accggaaagc gctcgacctc
     9361 gttgtagtag ccggtgtggt ccccgtgcgg accttcctcg gcggtttcct cgccgagcac
     9421 gccttcgagg atgatctcgg cgctcgccgg cacctgcagc gcgttgccca ggcatttgac
     9481 cagctcggtg cgcgccccgc gcagcaaccc ggcgaactgg tactccgaca gcgtgtccgg
     9541 gatcggcgcc accgccgcga gcatggtcgc cgggtcggcg cccagcgcga cggcgacggg
     9601 gaacggttcc tttgaaacag caacatggtc gcggtaatcg agggcgccgc cccggtgcgc
     9661 gagccagcgc atgatgacgc ggttgcggcc gatcacctgc tggcgataga tgccgaggtt
     9721 ctggcggggc ttggcgggcc cgcgggtcac cgtgaggccc caggtgatga gcggaccggc
     9781 gtcctgcggc cagcaggtct gcaccggcag ccgcgccaga tccacgtctt tcccttctag
     9841 gatgatctcc tgcgaggaag ctgtcgaaac cgtcttcggc ggcatattca ggagctgctt
     9901 ccagaccggg aacttctccc agaggtcgcg caggcccttc ggcggctcgg gctcgcgcag
     9961 gaacgcgagc atctggccga tctcgcgcag cgccgaccga ttctcctccc ccctggcgac
    10021 gcccatcgcc gccgcgacgc ggcgcacggc gccgaagagg ttggcgagca ccggaatctg
    10081 gtggccggcg ggccgctcga acagcagcgc cggcccgccc gcgcgcagca cgcggtcgca
    10141 gatctcggtc atttcgagcc gcggcgacac ctcggccttg atgcgcttca ggtcgccgtt
    10201 cgcttcgagc tgtgcgatga actcccgcag gtccgagtac ttcatcgcct gagggacatg
    10261 agccggtcgc tcaccgactc ggcgaagtcg agcagcagcg tgtcgcgccc gcgcgtcgag
    10321 tagagacgct cggcgccggg caggctgatc agcagcgtag tgctacccat ccacccgagc
    10381 gcgcacaaca cgccgccgat gatcacgcag ccgagcgccg cgaccggcag caggctcgcg
    10441 agggcctcga gcaggcgaaa gaagctgtgc agcgcgcccg gcacgccttg cgcgccagcc
    10501 gtggccatct cgccggcagc gccgcttgcg aacctgccga gcggtcctgc caccgcgagc
    10561 atcacgagcg cgatcaccac gagcgcgatt ccccagccga gcttgcgcgt atcgcgctgg
    10621 aaggccacgc ggaccgcggt cattccggca aggggcagcg tttcgacgtg gtgcgcgctc
    10681 cggtgcacga ggcagttcgg atgcagcgtg agctgtgtcc cgcgcagcag gccgctggcg
    10741 aattcgaagc gcgagactgc cgacacgggt tcgttcatga catgcccaga taaatgcccg
    10801 cgaagatcgc cgccccgacc cagttgttgt gcatgaatgc cttgaacgag ccttcgcggc
    10861 ttcggtcgcg aatcagccgc cagtggtaga gcatcatcgc ggccgcggct gccagccccg
    10921 cgtagtacgg ccaggacagc gaaagatatg caccgaggat tgcgaggatc aggaccatgc
    10981 tggcatagct cgccatcacc gcggcgacat cccagcggcc cagggtcagc gccgcggtgc
    11041 ggatgccgat gcgcgcgtcg tcgtcgcggt cgaccatcgc gtattcggtg tcgtaggcga
    11101 acgagtagaa gacatttgcc gcgaggagga gccagcactc gagcggcagg cgttcctgga
    11161 tcgcggcata ggccatggga atgccgaagc cgaaagcgat gccgaggatc gcctgcggaa
    11221 acgcgaagaa gcgcttggtg aacggataga gcgcggcgat ggcgagcgcg acgaacgaga
    11281 gcgcgatcgc gaagccgttg agaaacagca ccaggacgaa agcggcagca gcgagcacgg
    11341 cggccacggc gagcgcttcg cgcggcagga tctccccggc tgcgagcggg cgtcggcgcg
    11401 tacgctccac gtgcggatcg aagccgcggt cggcgtagtc gttgatggcg cagccggaag
    11461 agcgcatcag cagcgtgccg agaacgaaga tgatcaggat gtcgggccga ggccggccac
    11521 cggaggcgag ccataccgcc cacagcgtcg gccacagcag gagcagcgcg ccgatcggct
    11581 tgtcgagccg gatcagccgc tcgtaggcat cgaggcgctt ggcaatgaac gccagatcca
    11641 tactcgaagg ataactcagc ggataacccc cgcttctcat gcttcggcga gctcccgccg
    11701 ggagccgagc cagcgcgcca cgtgcgcgcg cgccgcggcc ggatcgcggt cgatcagctc
    11761 ggccgccgca tgcaccgcgg ccccgaccaa gtcctggtcg cgctcgagat ccgcgaagcg
    11821 caggagcggc gcgccgctct ggcgctcgcc aaggaactct cctggccccc gcaactcgag
    11881 atccttgcgc gcaatctcga acccgtccga acagtcacga acgatcccta gacgcttgcc
    11941 tgccgcttcc gacagcttcg cgccaaaaag caggatgcac acgctctctc gctggccgcg
    12001 accgatccgc ccgcgcagtt ggtgcagctg cgcaagtccg aagcgctcgg cgctctcgat
    12061 caccatcagc gaggcattcg gcacgtcgac gccgacttcg attaccgtgg tgcagacgag
    12121 cacctgcatc gctccctttt cgaacgcgtc catcacgagc gccttatcgc ccggcggcag
    12181 gcgcccgtgc agcagcccga cgcgcaaccc cttcagctcg ctcgtgagag acgcatgggc
    12241 atcgagcgcg gtctggagat caccctcctt cgactcctcg atcacggggc acacccaata
    12301 ggcctgctgc ccttctgcgc aggcttcgcg aatccgcttg aggacctctg gacgcttttc
    12361 cgccgagaac gttcgagtcc tgactggctg acgccccggc ggcatttcat cgatcgtcga
    12421 gacgtcgagg tcggcaaaat aggtcatctc cagggtacgc gggataggcg ttgcgctcat
    12481 catgagctgg tgcggcacga tagctcggga cgcgggaccc cccgtagagg ccgccttctt
    12541 acgcagcgcg aggcgctgct cgacgccgaa gcgatgctgc tcatcgacca cgaccaggcc
    12601 caggcgcgag aattccacgt ccttctggat cagggcatgc gtgccgatga gcacgcgccc
    12661 ttccagcatc tgcttcttct ctttcttggc catgccgccg cgcaggtagc ccaggcgcac
    12721 gccgagcggc gcgagccatt cgttgaattt gcgccaatgc tgctccgaca ggatctcggt
    12781 cggcgccatc accgcggcct gcgcacccga gtccaccgcg gcgaggcagg cgatcgcggc
    12841 gacgatcgtc ttgccgctgc cgacgtcgcc ctgtagcagc cgctgcatgg gatgcggttc
    12901 gcgcaggtcc cgcaggacct cgttcatggc gcgcatttga gctttggtca acgagaacgg
    12961 caacgtggag agaaagccct ttagcagagc gccgttcacc gtaagcaccg gcgcctgacg
    13021 cgagcggcgc cggcgataag caaagcgcat ggagagctgc tgcgcgagca gctcgtcgaa
    13081 cttgacgcgc ctccaggccg ggtggctgcg attcgaaagc acgccaagat cagtcccggg
    13141 gggcggccgg tgcaggaacc tcacactggc gccgaaatcg gcaaggttca gccgctgccg
    13201 aagccattgc ggaagcgtat cgtccaaggt ttcagcgtcg agcgactcca gcacacgcgt
    13261 acgcagctca tgctgcctca acttttcggt cgtcgggtag atcggcgtca aagtctgcgg
    13321 cagcggctcg ccctctttca cgaggcgata tctcgggtgt gccatctcaa cgcctaatcg
    13381 cccgcgccga acctcgccga atgcccgcac cttcaggccc tgctctgcgg cttgctggag
    13441 ctgccgcagc tggctgggga aaaagttgaa gaagtgaagt accgggccgt ccgtgtacac
    13501 gatcagttgg cgtgtcggcc ggtacatcac cttcgccttt gagacctttg cctcaacgtg
    13561 tatcggcgta ccgggtacca cggatccgag aggagtaagc gaggtctcgt cctcgtagcg
    13621 cagcggcagg tggagcacga agtcgaagcg cgagcgcagc ccgagcttct gcagttgctt
    13681 ttccagtccg cctttaatcg gcgtgcagga tcgcgtcgat ctcgacgatc gcgccgcgcg
    13741 gcaacccggc cacgccgacc gcggcgcgcg ccgggtaggg ctcgcgcacg tactgagcca
    13801 tgatctcgtt cacgcgcgcg aagtgcgcga gatcggtgag gtacacggtc atgcgtaccg
    13861 cctggtcgag gctcaggcct gccgcggcgg cgacggcggc gaggttgcgg aatacccggt
    13921 gcgtctgccc gtcaatgccc tccgcaagct gcatgctgcc tggatcgaga ccgatttgcc
    13981 ctgagagata cacggtgtcg ccagcccgga ccgcctgtga atacgtgccg atcgcctcgg
    14041 gcgcatcgcg cgtctggatc ggggttttca tcgggagtat cttagcctaa tgatttcgca
    14101 gctcgaaaag ctcctcggga cgccgcgcga cggcgcgctg ctgcgctact cgctcgggct
    14161 cgagcacgct aagggcggta atcatgaacg cgcgatcgaa tacttgcgcg acgcggttac
    14221 ccgggacccc ctctactcag ccgcgtggaa ggcgctcggc aagtcgctca gcgattcgaa
    14281 tcttcacgcc gacgcgctcg aggcgtaccg ccaaggcatc gcggcggcaa agcagaaggg
    14341 cgaccgtcag gccgagaagg aaatgaccgt gtttgcgaaa cgactcgaaa aactagggaa
    14401 acagccgggc tagcgcatcg cgcagccctg cgatgaagct ttccttgccg accagcgcgt
    14461 cggcgcccgc gcgctttgcg gcggccgccg cttccggcgt gtggaacagc gtcatcgcga
    14521 cgaccgccgg cgcgccgggt gcggccttca cctgcttcac catctccagt ccgcgcaccg
    14581 gcggcatgcc cagatcgagc agcagcacat ccggacccgc cgagctgacg agcgtaagcg
    14641 cctgcattgc ggtgttcgaa gtgccggcca ggatatagcc cggcaggtcc gccacgtggt
    14701 tcgcggccgc ggtcaggaag gccgggctgc catcggcaat cgcgatacgg cggagacgct
    14761 cgtcggacat tgggagccaa gatagagccg tacccgaggc acggctatcg gggccttccc
    14821 tagcctgcgc tgggaatttc ctgaggcgac tgcagcacag cccgcatcta gccctcgggc
    14881 ggcaccagcc cggttcgcag ggcgtatctc acaagcccgg cgacatcgcg gatgccgagc
    14941 cgctccatga tctgcgcgcg gtgggtctcc accgtcttca ccgacaggtt caggaggaac
    15001 gcgatttcct tcgtgctctt gcccccggcg acgagcttca gcacctcgcg ctggcgtgcg
    15061 gtgagcactt cgggcgcagt ctcgccgccg gtgcggtgca tgtagccctc gaccacctgg
    15121 cgcgagaccg agggactgag ccagctttcg ccgcgcatca cgctgcgcag cgcgagctcg
    15181 agctcgccgg tggcggcgtc cttcagcagg tagccgacgg cgcccgcgcg cagcgcctgc
    15241 agcacgtatt cctcgccgga gtgcatcgag aggatcacca cccgcacctc cggatggcgt
    15301 tcgcgcaggc gcgcggctgc ctcgaggccg gtgatgccct tcatcgctat gtccatcagg
    15361 acgacatcgg gctgggcgcg gttggcaagc tcgagcgcct cctcgccgct cgaggcttcg
    15421 cccaccacct ggacctctcg catcccctgc aggagcgagt ggataccggc gcgcaccagc
    15481 gcgtggtcgt cggcgagcag cacccggatc atccgaccgc ccgctccttt ccggccgcgc
    15541 cgagcggaaa cgttgccagc agaaccgtgc cctgcccggg cgccgagcgc agctcgaacg
    15601 agccgccggc gagcgccatg cgctcctcca tgccgatcag gccgaggctc gagccgttcg
    15661 ccgagcgctc gcgcaccgag tcgagctcga agccgcggcc gtcgtcgcgc accgagatcg
    15721 cgagccggcc cccggcggtg aacaggcgca cccaaaggtt gcgcgcgcgg gcatggcgca
    15781 gcacgttgtt gatcgcctcc tgcgcaacgc gaaagcaggc ggtctcggta tcgggggcga
    15841 gctcctgcgg cgcatcctcg gtctcgaagt gcgcctgcag gccggcgacg cgagcctggc
    15901 gatcgaggtg cgagcgcaac gccgccgcca ggccgaggtc gtcgagctgc ggcggccgca
    15961 ggctgatcga gaggtggcgc acacgctcga gcgtgtgctg cacggtgtcg acgcactcgt
    16021 cgacgcgcga gcgcgccgcc acctcgccgc cggcgcgcgc gaccgattcc agttggatct
    16081 tcagcgcagt gagcgcctgg ccgacgtcgt cgtgcaggtc gcgcgcgagg cgccggcgct
    16141 cctcctcctg cacctcgaac agccgccgcg acagcgcgcg cagccgctcg tgcgacagct
    16201 cgagctcgcg gttcaggcgc tcgagcgtct gctgcgtgcg cagccgctcg gtgatgtcgt
    16261 tgatctgcac cagccacgcc ggccggccgc gccaggcgac caggtggccg acgccggaaa
    16321 tgtcgatcgg ctcgccgctc ttcttcagat gacggaacgt gccgtgccgc acgtcgcccg
    16381 gctcgcggcg cacgaaggcg gagaacgagt cctgatcttc cggcggccgg atatcgaacg
    16441 ccgtcatggc gaggaattct tcgcgcgagt actcgtagag gtgcagcgcc gcttcgttga
    16501 ccgcgagaaa gcgcagcgac tcggcgtcga tgacccacat cggcagcggg ctgcgctcga
    16561 agagcatgcg gtagcgcccc tcgtgcggat cgatcttgtc gaccaggcgc tgcagcaggg
    16621 cggcgacttg gcgtagcggg ttcatcgaag cgcgaatggt aacgctggca cgcagtttgc
    16681 gcgtgcctgc tcctgcgcat gaatacccgc cgaaacggaa gctcttacca tctcttgcat
    16741 ccggaaggtt ccgccgaggc cgccgggctg cgctatgtga acgacgccat gcccggaatt
    16801 tcgcgccggc gcgcgggcgc ggtgtttcgc taccatggcc cagacggcgc cggggttcgc
    16861 gacccggcgg ttcttgcgcg catcaaggcg cttgccgtcc cgccggcctg ggagaaagtc
    16921 tggatctgcc cgcgcgacga cggccacctt caggcaaccg gacgcgacgc gcgcgggcgc
    16981 aagcagtacc gctaccaccc ccgctggcgc gaagtgcgcg acgagaccaa gtacggccgc
    17041 atgctctcgt tcgctcaagt cctgtcgcgc atccggcgcc gcgtccgccg ggatctcgcg
    17101 ctttccgggc tgccgcgcga taaggtgctt gccacggtgg tgcatctcct cgaaaccacg
    17161 cgcatgcgcg tgggcaacgc cgagtacgcg cgcgacaacg attcgttcgg cctcaccacg
    17221 ctgcgtacgc gccaagtgcg cgtgcgcggc tcgacgatgc agtttcgctt tcgcggcaag
    17281 agcggcgtct ggcacgagtt cgcgctgagc gaccgccggc tcgcgtcgat cgtgcgccgc
    17341 atgcgcgacc tgccgggcta cgagttgttc cagtacgtcg acgaagcggg cgagacccgc
    17401 gcggtcgact cggccgacgt gaacgcctac ctgaagagca tcgccggcaa ggaattcacc
    17461 agcaaggatt tccgcacttg ggccggtacg gtgctcgcgg ccgaggcgct gcatgagttc
    17521 ggcgccgagg ggctgacgaa agcgatcgag caggtggcgc ggctgctcgg caataccaag
    17581 gccgtgtgcc gcaagtgcta catccatccc gccgtcatcg acgcctgtct cgacggcagc
    17641 cttgggcaag cgatgaacgg ctgcagcgcg gaagcgtgcg tgaagcggct gatcgcgcgt
    17701 cggcgccggc cgcgctcgct cgtgcccgcc ctccagcagt cgctacgcaa gctgcgccga
    17761 cgcggcacgc agcttgcagg gcgtcggggg cgtatccgcg caactcaccc cattccctga
    17821 aggaaacctc atgacccgga acatcctttt cgcagcgatg ctcgcggccg caagcgcgtc
    17881 gttcgcccaa tccccccagc ccggacagac tacgccgagc gtgacgccgt cccagacgcc
    17941 tggcgtaacg ccgtctcaga cgcctgccgt aacgccctct caaacgccat caacgtccca
    18001 gaatcccgtc gtagcgccgc catcaggcgc gcctgtcgtc ggcaacacag cgcctgcgcc
    18061 cgtggtcgag ccacgctcga tcggcggcat gagcaggtgc gagaacgccc tcgccttcga
    18121 gaaggaaaag tgcctgcagg acgagcgcgc agccgcgacg ggctcggcca accgtccggc
    18181 cgccaccggc gccagtgccc cgaatagcgg cagccccgca ccgagcagca ccgcaacgcc
    18241 cgcgggcacc atggcgccgc agaacaccgc gcccaacccc gcacccgccg gcacagtgcg
    18301 ctagatcagc cgaggagcac cgcggcggag cgcggcacca ccaccggcgc gagcggcggc
    18361 ggcaccctcg gcggcgcgac gccgcgatag cggaaaaggc ccttagaaga aggctagcgc
    18421 ccgcgcctcg atgctctgcc gcggcggggc gcccagtggc gtcgacggat cgtcgaacga
    18481 cgcgtgcgga gtgaagcgcg cgcggccgtc cgactcgctg tcgaacacct tgaaaaccag
    18541 cgcctcgtcg cgccgcatct gcgggaaata gaaccagcgg tgcttcgggc tgtacttcag
    18601 gcgataggtc tgcccgatgc ggtgcggata gcgccgctcg gcgatgagca ggtcttctgg
    18661 cgcgacgctc ttcgagtccg cgatcgcgag cggattgcat tggatcggct ggttgatcgc
    18721 gcgccagacc tggatgatgg caaagcggcg cttgagcagg gtctcggcct cgtcgcccat
    18781 cagctcgcgt acgcggttcg gccctgacca ttcggtgtag tcgttgtgcg ccgagggcag
    18841 cggctcgcgg atggagcgcg cctcgcgctc ggcctcgtcg cccgagcgca gggtgtgatc
    18901 gaatacgacg gcccgcgcgg cacccgagac gctccgaatg agctctgaca cttccgggta
    18961 gtagacgagc ttgagctcgg ccttgtcgaa gaaattccgg accgcggtcc tttgttcgac
    19021 caacacgaag ccgttctcgt cgagggaaaa cggggtggag gaaatgcggc cattgcgtat
    19081 ttccacctca tggacttctt cggtgtccgc agtgcggcgc cggatgttgt tgttcgggcc
    19141 gaaggtcacg ttgacgagct tttcgccggt ggcggcggtg tagcggatgt ggaccagcat
    19201 ccccccaact atagacttgt cggctggtgc agggcgcgcg caatgtcgtc gtagaacgat
    19261 ttacctcgga gcaccggcac tgccgggacg ttccgggacg ggaaaggaaa ctcttacatg
    19321 cgccaagcgt ctcatgtgtt ccttcgcaga gacaccggca gctacttctt tcgctggacc
    19381 atccctgcaa cgattcgcca gcttctccgc ggacaccaag ctgatttgcc cgacggagcg
    19441 ggacgtttct tctcgaacgc gagcatagcg ccgcagtgtt tgctgctgat tccgctacgg
    19501 ttgggaatct ggtggagtgg aggaggatcg aactcccgac cttcgcattg cgaacgcgac
    19561 gctctcccag ctgagctacc accccgcggt tgcccgactg caggcggcgg attttaccgt
    19621 aatattcttt ccccatgcag atctcgccgc cgttcggata caaagaggtc gtcccgttcc
    19681 tgaagacgca gaaggtgcgc ctgctcgcgc ccggcgaggt gccggaattc gtgcagcggg
    19741 gcaacgcggt gccgatcagc cataccgagt tccagccgat cgcgcgccac tatccgatcg
    19801 tgttcaccac cggcgacgag ggcaagacct acgccgcggt cgccgtgctc ggcatctccg
    19861 ccggcgagaa cctgttctat gccgacagcc gctggtcgag cggcgcctac attcccgcct
    19921 atgcgcggcg ctatccgttc tgcatggcgc gggtgaacct gaacaaggtc gagcagaagg
    19981 accgcctcat ctgcgtggag aagtcatcgc tcgacgagga gggcggcgag gcgatgttcg
    20041 acgccgcggg acaagcgagc gacaagtgga agggcgtgga gcggctgctc gcggagtacg
    20101 aggccgatct cgagcgcatg cgcgagatgt gcgcgctgct cggcgactac gggctgctcg
    20161 agccgttcag catgcaggcc acgctcaaga aagaaaaggg cggcggctcg atgcagatga
    20221 gcggcatgca tcgcgtcgcg gagaagaacc tggagaacct gaacaccgcg cagctgaaga
    20281 acctgatgcg caagggcttc ctcgcgcgca tctacctgca cctgctctcg ctcgagaatt
    20341 tcgcccgcct gctcgaccgc aagggcgagc gctccgcggc ctagtcaccg aacgccgaca
    20401 cgctgtcgtg gccgcgatga cggcctcagg cgcgattgcg ccgtagcatt tatgcgccga
    20461 gaatgagccg caatgagggc gccatgagct attccgatct cgataaggcg cgcgagtgcc
    20521 acggcgcgct gcgggagatt ttcattcacc atcccggcgg ctgggcggac aaggaagcgc
    20581 tctacagggt ggagaagctc gcctggcagg cgatgacgct cgtgaaggac gccgagtgcc
    20641 aggagaagat gggcatcgtc gagcactatg ccgccgcgct ctattcggaa gccgagcacc
    20701 accggtggcg catgacctcg ctgcccggcg cggattttct caggctccag atcctgaggg
    20761 cgctggtcgc gtttcacggc cgcctcttcg ccatcgaggc cgcgcggcgc aacgatgtcg
    20821 acccgaatgc cgctctggcg cgcgctccgg cactcgagca gggttcggag cgcaccggct
    20881 gatcggcagg taggggtcct gcgacccagg cacgactcct gctccgtcgt agagacgagc
    20941 aggagtgcag gtgaacgagc gcaggaaaag ccgtgtaggc ctcaaggtga tccacaggca
    21001 ccggatcagc gacaaggtat tccgccgcgt ctccgagctc gtgatgatcg acggtcagcc
    21061 caaggccctc ctcggctgga tcaacctggg cgaggtgcgc accccgatct acctctgcga
    21121 tctcgacccg tccaagctgc accgttccgc caccagcgcc accaccttct attacgatga
    21181 gatgacggtc gatccgagat tcgaagcgct gggtccgggc ctgcgcgatg gcgacctgcc
    21241 gaccagcgcc gtttaacgag cgtacttcag ggcgtacctc aggtttttca ctctttctag
    21301 ccttcctcgc cgaccggtga ggaatttttg acgcggcaag gcggccgctt gcgcctacag
    21361 tagcgctcat gaaaaccctc ctcgccatcg ccgtcacggc agccctcacg ggctgcggtt
    21421 tggaaaccgc cagcaccgcc gccaccgccg cctcgatcaa gaagcaggag gtgcaagagg
    21481 cgcagaagac catgcataac gcgcagcaga ggatcgacgc ggcgacgcag cagatgcacc
    21541 agagcgcgga atctcgcgaa ccgcagtaag tcaccgcctg ccgcggccat cgaatttggc
    21601 cgatccgaca actcgttgta gtgtgtgctg ttgtcgggca agaataaatg ggggttggca
    21661 tgaaaaacca caggtggctg atcgttgtgg ggctgctggc ggcggtgacc tcgggtccgg
    21721 cggccgcgca actctacgcc ggcggctcaa tcggctattc gcagttcaag gatgtctgcg
    21781 tgcttgccaa tgttccgtgc gatgacaccg acacggcctg gcgcgctttc ggcggctatc
    21841 agttcaaccc gtactttgcg ctcgaggtgg gattcgccga cctcggcgcg accaccggct
    21901 cggggtcggt cggcagcttc agcgtcgagg cgaagcaggc ttgggacctg tccgcgcttt
    21961 tcaccatccc ggtgacgacc cgcttttcgt ttctcgccag aatcggtgca catcgcgtcc
    22021 gaaccacggt ggatcagcag gtgaccggcg ttggacccct acacgaggcc aagactggct
    22081 ccagttggac ctatggcgcg ggcgcggagt actcgttggg caagcttggc gtgcgcgccg
    22141 aatggcagcg ctacgacaac atcgccggcg ggaccctcgt cgaggacgac gtcgacgtcc
    22201 tcagcatcgg cctgctcttc aggttctgac cgcttccgcc cggggcgggt gggtgggccg
    22261 ggttggattc gaaccaacga ccaagggatt atgagtcccc tgctctgacc gactaagcta
    22321 ccggcccccc aaaaccgaga ttactgcaag cctttgtact tcggcgcgag cctggcgagg
    22381 aagccgtagc ctatgcccgc gagctcgtgg ccgcgaaaca ccagaggcgt gagctcgtcg
    22441 tcggtgatct tgtcgtcgcg cgccttgagg tcggtgtaat agaacagcac gtcgtacgcc
    22501 gcgccgtccg gcccggtgac gcgctccacg cgctgcgggt ttttcacctc caggtactgc
    22561 agccggccga ataggatatc gcccatcttg ccgccggcgc tcgcgtcgcc catcagcttc
    22621 tccacctgcg cgcgcggcgt cccgattgca agctgcgcga tgttgcgctt gttgttctcg
    22681 cgcagctgct cgatgctgtc gatgatcagc gtagagcaac cggcgagaac accggctgca
    22741 agaatggacg tccgccagat ccccatagca gaagggccgc tttcgcggcc ccttttttat
    22801 ccctcttgat cgaggaagct ttttagtcgc tcggaccgcg acgggtggcg cagcttgcgc
    22861 agcgccttgg cctcgatctg gcgtatgcgc tcgcgcgtga cgtcgaactg cttgccgact
    22921 tcttccagcg tgtggtcagt cgacatctcg atgccgaagc gcatgcgcag gaccttggcc
    22981 tcgcgcgggg tgagcgtgtc gagcacctcc ttggtgacat cccggaggct cgcgtaggtg
    23041 gcggcctcgg agggcgcgac ggtcgaggtg tcctcgatga agtcgccgag gtgcgaatcg
    23101 tcgtcgtcgc cgatcggcgt ctccatggag atcggctcct tggagatctt caggatcttg
    23161 cggatcttct cctcgggcat ttccatgcgc tgggcgagcg tcgccgggtc gggctcctgc
    23221 ccggtctcct gcaggatctg gcggctgatg cggttcatct tgttgatggt ctcgatcatg
    23281 tgcaccggga tgcggatggt gcgcgcctgg tcggcgatcg agcgcgtgat ggcctggcgg
    23341 atccaccacg tggcgtaggt cgagaacttg tagccgcggc ggtattcgaa cttgtc
//
LOCUS       DATBSZ010000117        10380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_904600, whole genome shotgun sequence.
ACCESSION   DATBSZ010000117 DATBSZ010000000
VERSION     DATBSZ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10380
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904600"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VIG70_07665"
     CDS             <1..819
                     /locus_tag="VIG70_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250465.1"
                     /translation="IAQKMIEASGLSVIVENRPGGSTLIGAREVQKSPPDGHTLLYTI
                     AIHVQLPHLYQAPPWDAFRDFTPITAGARSATVLTAHAALPFNTVPELVTYAKANPGK
                     LNYASFGAGTTSHLNGELLKRLAGIDIVHVPYKGAADAMKDLLAGTVQLYFDGPTTAI
                     ASAKTGKVKLIATAAETRKAALPDLPTMRETGYDVGMWGYLWFWGPAGMSPATLQAVY
                     QPLGRAIRHPEIAELFAKGGAEAAGMPPAEFAPLARELSERWGAVIRELGVKLD"
     gene            819..1961
                     /locus_tag="VIG70_07670"
     CDS             819..1961
                     /locus_tag="VIG70_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY8250466.1"
                     /translation="MSGPLSGIRVLEFESIGPAPFAGMLLADMGADVLVVDRPATTDL
                     GLKRERRHDVMMRGKRSVTLDLKSASAKEAALALAARADALIEGMRPGVMERLGLGPD
                     EVLGKNPKLVYGRMTGWGQDGPLAPRAGHDINYIAVAGVLHAIGRKGEAPVPPLNLIG
                     DFGGGGMLLGFGVACALLEAARSGRGQVVDAAMVDGASLLAAMFAGLLKAGTWSEKRG
                     ENILDTGAPWYNVYETKDGKYVSIGSIETRFYEELLQKLGAPDLGQHDRARWPEMRSL
                     FEKAFKAKPRDEWCKVFEGSDACFAPVLSWSEARRDSHNKSRKSFIEVSGVEQPAPAP
                     RFSRTPGKVRRAPPERGEDGQQALAEWGFTQEEIRNLSRLGLGIGE"
     gene            1964..2590
                     /locus_tag="VIG70_07675"
     CDS             1964..2590
                     /locus_tag="VIG70_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEY8250467.1"
                     /translation="MLGRLMPREGKFFDLFNLHAERIVEGSRELAAMIGTFSELEAHA
                     QRIDAAERAADKVTHETIALLHKTFITPFDRDQIHQLITTMDDILDLTQDVAESVALY
                     DLRKITPEAKQLADICHMSCERVAAVVGLLRNVKRTDAIMKICEEIDRLESDADRVMR
                     SALSKLFREENDLKQVIKLRVIYDLLETITDRCEDVANVIEGIVLENS"
     gene            2610..3626
                     /locus_tag="VIG70_07680"
     CDS             2610..3626
                     /locus_tag="VIG70_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY8250468.1"
                     /translation="MDGSVTHIGLGVVIALVLVALAFDFMNGFHDAANSIATVVSTRV
                     LKPYQAVVLAAFFNFVAVFVFQLSVAATVGKGIVDQGIVDHYVVFGALVGAISWNLIT
                     WYYGIPSSSSHALIGGIVGAGVAKAGTGMLIPAGILKTVAFIFISPLIGLVLGGTFLV
                     VISWLFVRSTPSRVDRLFRRLQLVSSSLYSLGHGGNDAQKTIGIIWMLLIAAGYTGAH
                     ESVPVWVVVACYVTIGLGTLFGGWRIVKTMGQRITKIRPVGGFCAELSGSIALFLATS
                     LGIPVSTTHTITGSIVGVGTAQSVSAVRWGVAGDLLWAWLLTIPCSAFVAAIAWALAR
                     AAFA"
     gene            complement(3635..4084)
                     /locus_tag="VIG70_07685"
     CDS             complement(3635..4084)
                     /locus_tag="VIG70_07685"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY8250469.1"
                     /translation="MRLALVALLLVAAQALAHNHGKGDIQVRHPWSRATPPGAKVAAG
                     YMEIRNLGSQPDRLLSVTSAAAQRVEMHVTQRDGEVMKMRQVQAFEIPARERITLRPG
                     GSHLMLVDLAQPLEKGARFVMKLRFERAGELEVEVEVQEIGSRHPRH"
     gene            complement(4081..5709)
                     /locus_tag="VIG70_07690"
     CDS             complement(4081..5709)
                     /locus_tag="VIG70_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY8250470.1"
                     /translation="MFSRHFAHWPPGQPRTLELPKASVYSNLVASTERHPQRAAIDYY
                     GSTLSYAQLKRQIDAAAGYLQQRLGVRRGDRVLLYAQNSPQFIIAYYAILRADAVVVP
                     VNPMNRTEELRHYVADSDAAVAIAGNDVLRELEPLGLLHVLPIVYADYLTADTDLPVP
                     EFLRTPGSPAWKNALAENLAPGPHLAGPGDLAVMPYTSGTTGKPKGCMHTHASVQATT
                     VPYLHWRGAQAQSVVLCAMPMFHVTGMQAGMNAPIHIGATLVVLSRWDRDCAAMQIER
                     AQVTNWSAITTMLVDFLSNPRLKEFDLSSLRVLGGGGAAMPEALARRLEDVIGLPFVE
                     GYGLSETMAPTHINPPHRPKRQCGGIPFFNTDARVIDVETLAELPPGKVGEIIVHGPQ
                     VFKGYWKQPEATEQAFLSHDGKRFFRTGDLGYYDEEGYFFITDRLKRMINCSGYKVWP
                     AEVESMLYAHPAIQEACVIAARDGYRGETVKALVVLKQDFSDKTSSQDIIAWARERMA
                     AFKVPRAVEFVDELPKTSTGKILWRKLQEEEYRK"
     gene            complement(5736..6794)
                     /locus_tag="VIG70_07695"
     CDS             complement(5736..6794)
                     /locus_tag="VIG70_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY8250471.1"
                     /translation="MRAVAAPMKRPLVTSIATVGVAPLLLLDLETDAGIVGRSYLFAL
                     AKHNLAPLAKLVEAMGEMVKGDEVAPFDLERKLRARHALLGVHNMVLFAMSGIDMAAW
                     DALGHALEQPLVRLLGARPRPIPAYNSKGLGILPENELAKEAGELVDEGFRAIKLRLG
                     RARAADDLNALKAVKKQIGPDITLMVDFNQALSVAEAIARGRMIDDEGGVTWIEEPVR
                     ADDFAGAARVRIEVKTPIQIGENFMGPEQMAQALAASACDYVMPDAERIGGVTGWMRA
                     AALAQGAGMEMSSHLFPEVSCHLLAATPTCHWLEYVDWADPVLEQSVQLKDGHVLVPE
                     SPGIGIRWNERAVERYQI"
     gene            complement(6812..7561)
                     /locus_tag="VIG70_07700"
     CDS             complement(6812..7561)
                     /locus_tag="VIG70_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY8250472.1"
                     /translation="MGWIVFEQPARRNAINGAMWRGIAPAVKQFDADAEVRCVAFRGA
                     GVEAFSAGADISEFEKTRAATESVGEYDGLLDQVLHAIQDSRKPSVAMIYGYCLGGGV
                     EIALACDLRYCAASAQFGIPAAKLGLAYNVEGHRRLLETVGHARAREIMFLGRRYDAE
                     QALAMGLVHKVLPSDALESFVGEVLQTLCENAPLSIANTKTILEEYVKSSGTPDARRM
                     REAMERCARSEDYREGRRAFMEKRKPRFTGR"
     gene            complement(7588..8478)
                     /locus_tag="VIG70_07705"
     CDS             complement(7588..8478)
                     /locus_tag="VIG70_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8250473.1"
                     /translation="MPTLAADDGVKLYYEEVGSGAPIVFVHEFGGDHRSWEPQVRHFS
                     RRYRCIAYNARGYPPSDVPEDFERYSQARARDDIRCVLDALGIQRAHVVGLSMGAFAT
                     LHFGMAYGYRATSITVAGGGYGSHPAQYRQFQADSKKNADLIRREGMAKFVSTYGHGP
                     QRVQLELKDPRGFAEYLRQFEEHSPVGAANTLLGVQNRRPSFYDLTAEMARIDVPTLI
                     MSGDEEEPCLEVNLLMKRVIPTAGLAILPRSGHAINLEEPALFNRLLEDFFHQVEAGR
                     WGPRDPRSTLPSVYGPSGKP"
     gene            8493..9470
                     /locus_tag="VIG70_07710"
     CDS             8493..9470
                     /locus_tag="VIG70_07710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEY8250474.1"
                     /translation="MKAVLCKQFGPPDSLVVEDVASPRAGPGEVVVTMKAASVNFPDV
                     LIIQNKYQFKPPLPFSPGSELAGVVKEVGADVQNVRPGDKVIAFTTYGAFAEEVKTEA
                     ARLMPLPDRMDFVTGAAFLLTYGTTDHALRDRAALAAGETLLVLGASGGVGLAAVEIG
                     KALGARVIACASSEDKLAVCRSHGADATINYAAEDLRERLKALTEGRGADVIYDPVGG
                     AYSEPAFRSIAWRGRHLVVGFAAGEIPKLPLNLALLKGAAVVGVFWGDFARREPQRFA
                     ASVGQLTRWFAEGRLKPHVSQTLPLEKAAEALKLMAARQVKGKLVLTAG"
     gene            complement(9457..9978)
                     /locus_tag="VIG70_07715"
     CDS             complement(9457..9978)
                     /locus_tag="VIG70_07715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8250475.1"
                     /translation="MAVELRAARPEDLPAIHSIYAHHVLHGLASFEEEPPSVDELGRR
                     LRDVTQRGLPYLVAEYGLEVAGYGYCSPYRARSAYRYALEDSIYIRHDMAGRGIGRRL
                     LDELIRRCEGLGYRQMIAVIGDSANAASIGVHAACGFLRVGTLRSVGFKFGRWVDSVY
                     MQRPLGAGDATPP"
     gene            complement(9980..>10380)
                     /locus_tag="VIG70_07720"
     CDS             complement(9980..>10380)
                     /locus_tag="VIG70_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY8250476.1"
                     /translation="EGGRLEMVEQNALRFHDDIAYDDIYNGLVVDNAEGDRLARALGG
                     KRVMFLANHGVIVVGPTVAEAFDALYYLERACRLQVLARSMGGKFRPVRPEVVRDAYR
                     MMLADAPKYAGAHFEALKRILDREEPAYSR"
BASE COUNT         1637 a         3516 c         3499 g         1728 t
ORIGIN      
        1 atcgcccaga agatgatcga agcctccggc ctgagcgtga tcgtcgagaa ccggccgggc
       61 ggttccaccc tcatcggcgc gcgcgaagtc cagaagtcgc cgcccgacgg tcacacgctg
      121 ctctacacga tcgcgatcca cgttcagctg ccgcacctgt accaggcgcc gccgtgggat
      181 gcgttccggg atttcacgcc gatcaccgcg ggcgcccggt cggcgaccgt gctcaccgcc
      241 cacgccgcgc ttccatttaa taccgtgcct gagctggtga cctatgcgaa ggcgaatccc
      301 ggcaagctga actacgcctc attcggcgcc ggcacgacgt cgcatctcaa cggcgagctt
      361 ttgaaacgcc tcgccggcat cgacatcgtc catgtgccgt ataagggtgc cgccgacgca
      421 atgaaagacc tgctggccgg cacggtgcag ttgtacttcg acggccctac gaccgcgatc
      481 gccagtgcga agaccggcaa ggtaaagctc atcgccactg ccgccgagac gcgcaaggct
      541 gcgttgcccg acctgccgac gatgcgcgaa accggctacg acgtcggcat gtggggctat
      601 ctctggttct gggggcctgc cggcatgtcg ccggcgacct tgcaggcggt ctatcagccc
      661 ctgggcaggg cgatccggca tccggagatc gcggagctct tcgccaaggg cggggccgaa
      721 gcggcgggca tgccgcccgc ggagttcgcc ccgctggcgc gcgagctgtc ggagcgctgg
      781 ggcgcggtga tccgcgagct cggcgtgaag ctcgattgat gagcgggccg ctgagcggca
      841 tccgggtgct cgagttcgag tcgatcggcc ccgcgccttt cgcgggcatg ctgctcgccg
      901 acatgggcgc cgacgtgctg gtcgtcgacc gccctgccac cacggacctc ggcctcaagc
      961 gcgagcgccg gcacgacgtc atgatgcgcg gcaagcgctc ggtgacgctg gacttgaaat
     1021 ccgcaagcgc gaaggaagcg gcgctcgcgt tggccgcaag ggcggatgcc ttgatcgaag
     1081 ggatgcgccc gggagtgatg gagcgcctcg gccttggtcc cgacgaggtg ctcgggaaaa
     1141 acccgaagct ggtttatggc cgcatgacgg gctggggcca ggacggaccg cttgcgccgc
     1201 gcgccgggca cgacatcaac tacatcgcgg tcgcgggcgt gctgcacgcc attggccgca
     1261 agggcgaagc gccggtgccg ccgctcaacc tgatcggcga cttcggcggc gggggcatgc
     1321 tgctcggctt cggcgtcgcc tgcgcgctgc tcgaggccgc gcgctccggg cgcggccagg
     1381 tggtcgacgc ggcgatggtc gacggcgcct cgctgctggc ggccatgttc gcggggcttc
     1441 tcaaggccgg cacctggagt gaaaagcgcg gcgagaacat cctcgatacc ggagcgccct
     1501 ggtacaacgt gtacgagacg aaagacggca agtatgtgtc gatcggctcg atcgagaccc
     1561 gcttctacga agaattgctg cagaagcttg gcgcaccgga cctgggacag cacgatcggg
     1621 cgcgctggcc ggaaatgcgc tccctgttcg agaaagcttt caaggcgaaa ccccgcgacg
     1681 agtggtgcaa ggtgttcgag gggtcggacg cgtgcttcgc gcccgtcctg tcctggtcgg
     1741 aggcgcggcg cgattcgcac aacaagtcca ggaaaagctt tatcgaggtg agcggggtcg
     1801 agcaacccgc gccggcgccg cgcttctcgc gcacgccggg caaggtgcgc agagcgccgc
     1861 ccgagcgcgg cgaggacggg cagcaggcgc tcgccgagtg gggtttcacg caggaagaaa
     1921 tcaggaacct gtcgaggctg ggcctcggga taggggaata ggaatgcttg gacgcctgat
     1981 gccccgcgag ggcaagttct tcgatctgtt caacctgcac gccgagcgca tcgtcgaagg
     2041 cagccgcgag ctcgccgcga tgatcggcac cttcagcgag ctcgaggcgc acgcgcagcg
     2101 gatcgacgcc gccgagcgcg ccgccgacaa ggtcacgcac gagaccatcg cgctcctgca
     2161 caagaccttc atcacgccgt tcgaccgcga ccagatccac cagctgatca cgaccatgga
     2221 cgacatcctc gacctgaccc aggacgtcgc cgagtccgtg gcgctctacg acctgcgcaa
     2281 gatcaccccg gaggcgaagc agctcgccga catctgccac atgagctgcg agcgcgtggc
     2341 ggccgtcgta gggctgctgc gcaacgtcaa gcgtaccgac gcgatcatga agatctgcga
     2401 ggagatcgac cgcctcgagt ccgacgccga ccgcgtgatg cgctcggcgc tctccaagct
     2461 gttccgcgag gagaacgacc tcaagcaggt gatcaagctg cgcgtgatct acgacctgct
     2521 cgagacgatc accgatcgct gcgaggacgt ggccaacgtc atcgagggta tcgtcctcga
     2581 gaattcctga tgttgcgggt cttcgtctaa tggacggcag cgtcacccac atcggcctcg
     2641 gggtggtgat cgcgctcgtg ctggtggcgc tcgccttcga cttcatgaac ggcttccacg
     2701 atgcggcgaa ctcgatcgcc acggtcgtgt cgacgcgcgt gctcaagccc taccaggcgg
     2761 tcgtcctggc ggcgttcttc aatttcgtcg ccgtgttcgt gttccagctg agcgtggccg
     2821 ccacggtcgg caaggggatc gtcgaccagg ggatcgtcga tcattacgtg gtgttcggcg
     2881 cgctggtcgg cgccatctcg tggaacctca tcacctggta ctacggcatt ccctcgtcgt
     2941 cctcgcacgc gctgatcggc ggcatcgtcg gcgcgggggt ggccaaggcg ggcaccggga
     3001 tgctcatccc ggcgggcatc ctcaagaccg tagcgttcat cttcatctcg ccgctgatcg
     3061 gcctcgtcct cggcggcacg ttcctggtcg tgatctcttg gctcttcgtg cgcagcacgc
     3121 cgagccgggt ggaccgcctg ttccggcgcc tgcagctcgt gtcctcgtcg ctctacagcc
     3181 tcggccacgg cggcaacgat gcccagaaga ccatcggcat catctggatg ctgctgatcg
     3241 ccgccgggta caccggcgcg cacgaaagcg tgccggtgtg ggtcgtggtc gcctgctacg
     3301 tcacgatcgg cctgggcacg ctgttcggcg gctggcgcat cgtcaagacc atgggacagc
     3361 gcattaccaa gatccggccg gtgggcgggt tctgcgccga gctgtcgggc tcgatcgccc
     3421 tgttcctcgc cacctcgctc ggcatcccgg tctccaccac gcacaccatc accgggtcca
     3481 tcgtcggggt gggaacggcg caatcggtgt ccgcagtgcg ctggggcgtg gcgggcgatc
     3541 tgctgtgggc gtggctgctg accattccct gctcggcctt cgtcgccgcg atcgcctggg
     3601 cgctcgcgcg cgctgcgttt gcctgatggc gcgctcagtg ccggggatgc cgcgagccga
     3661 tttcctggac ttcgacttcg acctccagct cgccggcgcg ctcgaagcgc agtttcatca
     3721 cgaagcgtgc gcccttctcc agcggctgcg cgagatcgac cagcatcagg tgcgacccgc
     3781 cggggcgcag cgtgatgcgc tcgcgcgccg ggatctcgaa ggcctgcacc tgccgcatct
     3841 tcatgacctc gccgtcgcgc tgcgtgacgt gcatctccac gcgctgcgcc gccgcgctcg
     3901 tcaccgatag cagccggtcg ggctgcgagc ccaggttgcg gatctccatg tagcccgcgg
     3961 cgaccttcgc gccgggcggc gtcgcgcgcg accagggatg gcgaacctgg atgtcgccct
     4021 tcccgtgatt gtgggcgagg gcctgcgccg cgacgagcag gagggccacc agggccagcc
     4081 tcattttcta tattcctcct cctgcagctt gcgccacagg atcttgccgg tcgaggtctt
     4141 gggcagctca tcgacgaact ccaccgcgcg cggcaccttg aacgccgcca tccgctcgcg
     4201 cgcccaagcg atgatgtctt gcgagctggt cttgtcgctg aagtcctgct tcaacaccac
     4261 cagcgctttc accgtctcgc cccggtagcc gtcgcgcgcg gcgatgacgc aggcctcctg
     4321 gatcgcggga tgcgcgtaga gcatggattc gacctcggcc ggccagacct tgtagcccga
     4381 gcagttgatc atgcgcttca gccggtcggt gatgaagaag tagccttcct cgtcgtagta
     4441 gccgaggtcg ccggtgcgga agaagcgctt gccgtcgtgc gacaggaacg cctgctcggt
     4501 cgcctccggc tgcttccagt agcccttgaa gacctgtggg ccgtgcacga tgatttcgcc
     4561 cactttgccg gggggaagct cggccagcgt ttccacatcg atgactcgcg catcggtgtt
     4621 gaagaaagga atgccgccgc actggcgctt gggccggtgc ggcgggttga tgtgggtggg
     4681 cgccatggtc tccgacaggc cgtagccttc gacgaacggc aggccgatca cgtcctccag
     4741 ccggcgcgcc agggcctcgg gcatcgccgc gccgccgccg ccgaggacgc gcagcgagga
     4801 aaggtcgaac tctttcaatc tcggattcga gaggaagtcg accagcatgg tggtgatcgc
     4861 cgaccagttg gtgacctgtg cgcgctcgat ctgcatcgcc gcgcagtcgc gatcccaacg
     4921 cgacagcacc accagcgtgg cgccgatatg gatcggcgcg ttcattccgg cctgcattcc
     4981 ggtgacgtgg aacatcggca tggcgcagag cacgacgctc tgcgcctgcg cgccgcgcca
     5041 gtggaggtac ggcaccgtgg tcgcctgcac gctcgcatgg gtgtgcatgc agcccttggg
     5101 ctttcccgtc gtgcccgagg tgtacggcat gactgcgagg tccccgggcc ctgcgaggtg
     5161 cggcccggga gcgaggttct cggccagggc atttttccac gctggcgacc ccggcgtccg
     5221 gaggaactcg ggaacgggaa ggtcggtatc agcggtcaga tagtcggcgt acacgatggg
     5281 cagcacatgc agcaggccga gcggctcgag ctcgcgcagc acgtcgttgc ccgcgatcgc
     5341 caccgccgca tcggagtccg cgacgtagtg gcgcagctcc tcggtacggt tcatcgggtt
     5401 gaccggcacg accaccgcgt cggcgcgcag gatcgcgtag taggcgatga tgaactgcgg
     5461 gctgttctgc gcgtagagga gcacgcggtc gccgcgccgc acccccaagc gctgctggag
     5521 atagcccgca gccgcatcga tctgccgctt gagctgggca tagctcaggg tgcttccgta
     5581 gtagtcgatg gctgcgcgct gcggatgccg ctcagtgctc gccaccaggt tcgagtagac
     5641 gctggccttg ggcagttcca gggttcgagg ctgccccggg ggccagtgcg cgaaatggcg
     5701 ggagaacatt taggacgcta gaacccgcgt gaacatcaga tctggtagcg ctcgaccgcc
     5761 ctctcgttcc agcggatgcc gatgccggga ctctccggca ccaggacatg gccgtccttc
     5821 agctggacgg actgctcgag caccgggtcg gcccagtcga cgtattcgag ccagtggcaa
     5881 gtcggcgtgg cggcgagaag atggcagctc acctcgggaa agagatggct cgacatctcc
     5941 atcccggcgc cctgggcgag agccgctgcg cgcatccagc cggtaacgcc gccgatgcgc
     6001 tccgcgtcgg gcattacgta gtcgcaggcg ctcgccgcga gcgcctgcgc catctgctcc
     6061 ggccccatga agttctcgcc gatctggatc ggggttttca cttcaattct cacgcgtgca
     6121 gctcccgcga aatcatcggc cctgaccggc tcctcgatcc aggtcacccc gccctcgtcg
     6181 tcgatcatgc gcccgcgcgc gatcgcctcg gcgacgctca gcgcctggtt gaagtcgacc
     6241 atcagggtga tgtcggggcc gatctgcttt ttgactgctt tgagcgcgtt caaatcatcg
     6301 gctgcgcgcg cccttcccag ccgcagcttg atcgcgcgga agccttcgtc gacgagctcg
     6361 cccgcctcct tcgccagctc gttctccgga aggattccga ggcccttcga gttgtacgca
     6421 ggtatcggcc gcggccttgc gcccagcagc cgaacgagcg gctgctccag cgcgtgtccc
     6481 agcgcgtccc aggcggccat gtcgatcccc gacatcgcga acagcaccat gttgtgcacc
     6541 ccgagcagcg cgtggcgggc gcggagcttg cgctcgaggt cgaagggcgc gacttcgtcg
     6601 cccttgacca tctcgcccat cgcctcgacg agctttgcga gcggcgcgag attgtgcttc
     6661 gccagggcga agaggtaaga gcgcccgacg atgccggcgt cggtttccag gtcgaggagc
     6721 agcagcggcg caacgccaac ggtggcaatg ctcgtgacca gcggccgctt catcggcgcc
     6781 gcaaccgcgc gcatccgcac gccagttatt ttcatcgacc cgtaaagcgg ggcttgcgct
     6841 tctccatgaa agcgcgccgg ccctcccggt aatcctcgct ccgcgcgcaa cgctccatcg
     6901 cctcgcgcat gcgacgagcg tcgggcgtgc cgctggattt cacgtactcc tcgaggatgg
     6961 tcttggtatt tgcgatcgac agcggcgcgt tctcgcacag ggtttgcagg acctcgccca
     7021 cgaagctctc cagcgcgtcg ctcggcagga ccttgtgcac caagcccatg gccagggcct
     7081 gctcggcgtc gtagcgccgg ccgaggaaca tgatctctct cgcgcgcgcg tggccgacgg
     7141 tctcgagcag gcgtctgtgg ccctcgacgt tataggcgag gccgagcttc gccgccggga
     7201 tgccgaactg cgccgacgcc gcgcaatagc gcaggtcgca ggcgagcgcg atctcgacgc
     7261 cgccgccgag gcagtagccg tagatcatcg ccaccgaggg cttgcgcgag tcctggatcg
     7321 cgtgcagcac ctggtcgagc aggccgtcgt actcgcccac cgactcggtc gccgcccggg
     7381 tcttttcaaa ttccgaaatg tcggcgcccg cggagaaagc ctccacccct gccccgcgga
     7441 acgcgacgca gcgcacttcc gcatcggcgt cgaactgctt caccgccggc gcgatgccgc
     7501 gccacatggc gccgttgatg gcgttgcgcc gcgccggctg ctcgaacacg atccacccca
     7561 ctgcccctcg tttttcgacg cgcagcgtca cggttttccg gagggtccgt agacggaagg
     7621 aagggtcgag cgcggatcgc gcggtcccca tcgcccggct tcgacctggt ggaagaagtc
     7681 ctcgagcagc cggttgaaga gcgccggctc ctcgaggttg atggcgtgcc cgctgcgcgg
     7741 cagtatcgcg agcccggcgg tcgggatgac gcgcttcatg agcaggttga cctcgaggca
     7801 gggctcctcc tcgtcgcccg acatgatgag cgtcggcacg tcgatgcgcg ccatttcggc
     7861 ggtcaggtcg tagaacgatg gccgccggtt ctgcacgcca agcagcgtgt tggcggctcc
     7921 gaccggcgag tgctcctcga actggcgcag gtactcggca aagccgcgcg ggtccttgag
     7981 ctcgagctgc acgcgctgcg ggccgtggcc gtaggtcgag acgaatttcg ccatgccttc
     8041 gcgccggatc aggtcggcgt tcttcttcga gtcggcctgg aactggcggt actgcgccgg
     8101 gtgcgagccg tagccgccgc cggcgacggt gatcgaggtc gcgcgatagc cgtaggccat
     8161 gccgaagtgc agcgtggcga aggcgcccat cgacaggccg acgacatgcg cgcgctggat
     8221 gccgagcgcg tcgagcacgc aacggatgtc gtcgcgcgcg cgcgcctgcg agtagcgctc
     8281 gaaatcctcc ggcacgtccg acggcgggta gccgcgcgcg ttgtaggcga tgcagcggta
     8341 gcggcgcgag aaatggcgca cctggggctc ccagctgcgg tggtcgccgc cgaactcgtg
     8401 cacgaacacg atcggcgcgc cgcttcccac ttcttcgtag tagagcttga cgccgtcgtc
     8461 ggcggcgagg gtaggcataa tgcaagccta cgatgaaagc ggtgctctgc aaacagttcg
     8521 gcccgcccga ttcgctcgtg gtcgaggacg tggcctcgcc gcgggctggg ccgggcgagg
     8581 tcgtggtgac gatgaaggcg gcgagcgtca actttcccga cgtcctcatc atccagaaca
     8641 agtaccagtt caagccgccg ctgccgttct cgcccggcag cgagctcgcc ggcgtggtga
     8701 aggaggtcgg cgcagacgtg cagaacgtcc ggccgggcga caaggtgatc gccttcacga
     8761 cctacggcgc tttcgccgag gaggtcaaga ccgaggcggc gcggctgatg ccgcttcccg
     8821 accggatgga cttcgtcacc ggggcggcgt ttctcctgac ctacggcacc accgaccacg
     8881 cgctgcgcga ccgcgccgcg cttgccgccg gcgagacgct gctcgtactc ggcgcctccg
     8941 gcggcgtcgg cctcgccgca gtcgagatcg gcaaggccct cggcgcgcgg gtcatcgcct
     9001 gcgcctcgag cgaggacaag ctcgccgtgt gccgcagcca cggcgccgac gccacgatca
     9061 actatgcggc cgaggacctg cgcgagcggc tcaaggccct gaccgaaggc cgcggcgccg
     9121 acgtgatcta cgacccggtc ggcggagcgt acagcgagcc cgcgttccgc tcgatcgcct
     9181 ggcgcggccg ccacctcgtg gtcggtttcg ccgccggcga gattccgaag ctgccgctca
     9241 acctcgcgct gctgaaaggc gccgcggtcg tcggcgtgtt ctggggcgat ttcgcgcgcc
     9301 gcgagccgca gcgatttgcc gccagcgtcg ggcaactgac cagatggttt gccgaagggc
     9361 ggctcaagcc ccacgtgtct cagacgctgc cgctggaaaa ggcggccgaa gcattgaagc
     9421 taatggccgc tcgccaggtg aaaggaaagc tcgtccttac ggcggggtag cgtcgcctgc
     9481 gcccagaggg cgttgcatat acacgctgtc cacccagcgc ccgaacttga agcccaccga
     9541 gcgcagggtt cccactcgca ggaaaccgca cgcggcgtgc acgccgatcg aggcggcgtt
     9601 ggcgctgtcg ccgattacgg cgatcatctg gcggtagccg agcccctcgc agcgccggat
     9661 aagctcgtcc aggagccgcc tgccgatgcc gcgtcccgcc atgtcgtgcc ggatgtagat
     9721 cgaatcctcg agcgcatagc ggtaggcgct gcgcgcccgg tacggcgagc aatagccgta
     9781 gcccgccacc tccaggccgt attcggcgac caggtagggc agtccgcgct gggtcacgtc
     9841 gcgcaagcgg cgtccgagct cgtccaccga aggcggctct tcctcgaagc tggcgagacc
     9901 gtgaagcacg tggtgcgcgt aaatcgagtg gatcgccggc agatcctcgg gacgcgccgc
     9961 ccgcagctcg accgccactt caacgggaat aagccggctc ctcgcggtcc aggattcgct
    10021 tcaacgcctc gaaatgcgcg cctgcgtact taggcgcgtc ggcgagcatc attcgatagg
    10081 cgtcacgaac cacctccggc cgcaccgggc ggaactttcc acccatcgag cgggcgagga
    10141 cctgcaggcg gcacgcacgc tcgaggtagt acagggcgtc gaacgcctcg gcgaccgtag
    10201 ggccgaccac gatcacgccg tggttggcga ggaacatgac gcgcttgccg ccgagcgccc
    10261 gcgccaagcg atcgccctcg gcgttgtcga cgaccagccc gttatagatg tcgtcgtagg
    10321 cgatgtcgtc gtgaaagcgc agcgcgttct gctccaccat ctccagcctg ccgccctcga
//
LOCUS       DATBSZ010000118         6436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1724200, whole genome shotgun sequence.
ACCESSION   DATBSZ010000118 DATBSZ010000000
VERSION     DATBSZ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724200"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..917)
                     /locus_tag="VIG70_07725"
     CDS             complement(138..917)
                     /locus_tag="VIG70_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY8250477.1"
                     /translation="MSANLKFKSTPIRERVNKEEWQMRVDLAAAYQLASIYKWSDLIY
                     THFSARLPGTEEFLINPYGMMFDEITASNLVKIDWEGKIIEDPLGMGVNQAGFVIHSC
                     VHAARPEIGCVIHTHTRAGVAVSAMKCGLLPISQHAMLVHADITYHDYEGVALYKDEQ
                     ARMAADLGKTSKAMILRNHGLLALGETVRGAFEVMYYLDAACQIQVDACAGGVHNVQL
                     MSEQAAKAAAATLNREDRPANYKDWPALLRMLDRRGIRYAD"
     gene            940..1893
                     /locus_tag="VIG70_07730"
     CDS             940..1893
                     /locus_tag="VIG70_07730"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8250478.1"
                     /translation="MRCAALLLAPFVACAAVAQPSCLKSPSEKYRGPMFDAMAQVESR
                     MAPRVLPGMDQAGVTTMALFARQHPKRNGEPDVLALKRRYPDRFVLGTPKRFDQHKDL
                     DNGFVRDTVAELDAGRYRFVGEILFAHADKAHGDQTMTGERYVAPEGKNVRRLMSALE
                     KRAAPVMTHWEVYNWSRDWPSFDALYAAFPGVTFIWPHAGFGSAGQVETVLASRPNVV
                     VTLSKKEKDPDSVTDKNLQAELGPAIVDECGAILADWRGLLEKYPDRFLFATDAHKDQ
                     RWLKYEKVVERWRAILGQLPDPLAERIAWRNAERVYAARRP"
     gene            complement(1872..2828)
                     /gene="dusA"
                     /locus_tag="VIG70_07735"
     CDS             complement(1872..2828)
                     /gene="dusA"
                     /locus_tag="VIG70_07735"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEY8250479.1"
                     /translation="MPDKRISVAPMMDVTDRHCRFFLRQVNSRAGLYTEMITSGALIH
                     GDVRRHLEFSEEEHPVALQLGGSEPDDLARCAKLGEQYGYDEINLNIGCPSERVQRGA
                     FGACLMAEPALVASCVQRIRDVVSKPVTVKHRVGIDRVEDYSFVRDFVGTVAQAGCKT
                     FIVHARNAVLKGLSPKENREIPPLKYDYVYRLKRDFPSLEIVINGGITSLPQIDLHLK
                     SVDGVMIGRAAYSNPWFLADAGKTRAQVVAAMHEYAKSVDSLRHVTRHMLGLYHGMPR
                     ARLWRQMLSDSEQLKRNRPDLLLEALDAAEGQDLENQGLRAA"
     gene            complement(2818..3432)
                     /locus_tag="VIG70_07740"
     CDS             complement(2818..3432)
                     /locus_tag="VIG70_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY8250480.1"
                     /translation="MSLELYLAYVLACAVIALIPGPTVTVIIANSLRHGARAGLLNVA
                     GTQLGLALMMAILVVGLSSVIAAMGWLFEWLRWAGAAYLVWIGWKLLRSPDLLEQGEN
                     AALPRGGFFLQGFLVLMANPKALLWFGAFIPQFVDPSGNYVAQIVLLGVTAMAVALVS
                     DGAYAVLTGRAGAFLSKKRIRLVSRLSGGFLIGGGIWLALTRAR"
     gene            complement(3437..4087)
                     /locus_tag="VIG70_07745"
     CDS             complement(3437..4087)
                     /locus_tag="VIG70_07745"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEY8250481.1"
                     /translation="MRLFFLLLAFVATPALAQYNGPAVETCRAYAKSEAAREGTRAKD
                     IVFERDQNLQIERYTRKLGSQFVSSILRGNGAVVLEGAPSAELSFICLLADDKRAVFF
                     EWLPRANVGPLTQCTRDGALRDKPRPCLELLLQVADNDLLQVYALHFQDARERDHNAG
                     NENAVTSFRKANDEWRQYRDSECARRRDHAPKGVTPDDYHLACMVELTRRRAFDMR"
     gene            complement(4084..5055)
                     /locus_tag="VIG70_07750"
     CDS             complement(4084..5055)
                     /locus_tag="VIG70_07750"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY8250482.1"
                     /translation="MKICVYGAGAIGGLMAAWLARSGHEVSVVARGAQLDAIRRAGLR
                     VQSKGKTESFSIKAESDPATLGAQDYVLVTVKAQSLTHVAETIAPLLGKDTSVVTAMN
                     GVPWWFFHGLKGRDAHLESLDPGGKLSRAIPAERVVGCVIHLAASTPEPGLVSHNMGS
                     KLILGEPGGRNTARTKRIAEAFAAAGFEIVVTDAIEKEFWVKLLGNVSFNPVSALTVT
                     TADQLIQNQEVKAYMVEIMREVLAIGRAVGVDADIDPEARIDMARALGRFKTSMLQDL
                     EAGKPLEIDGLLAGTLEIARQAGVRAPFTESLFGLIRARAQSTGQYR"
     gene            5133..5777
                     /gene="msrA"
                     /locus_tag="VIG70_07755"
     CDS             5133..5777
                     /gene="msrA"
                     /locus_tag="VIG70_07755"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY8250483.1"
                     /translation="MKKMKTLLPKESTLPGRASEMRVPAKHFVNGHALKAPFPEGMQL
                     AQFGLGCFWGAERVFWETPGVYSTAVGYAGGDTKNPTYEEVCSGFTNHTEAVLVVFDP
                     KIVTYEELLKVFWESHDPTQGMRQGNDAGSQYRSAVYTYGEAQAKAAARSKEMYEKAL
                     KAKGYGPITTEIRAAPEFYYAEDYHQQYLAKNPGGYCGLGGTGVSCPAGVTAKV"
     gene            5777..6157
                     /locus_tag="VIG70_07760"
     CDS             5777..6157
                     /locus_tag="VIG70_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY8250484.1"
                     /translation="MRLDKWLWAARFFKTRSLAQQAIAAGRVKLGDARVKASHLVKPS
                     DSLAIRVGDFEWQIVVSALSDKRGPAEQARKLYQETEASRAERERRMDLRRWGAEPAR
                     GLKGRPTKRDRRKFEDFTSPPDES"
     gene            6197..>6436
                     /locus_tag="VIG70_07765"
     CDS             6197..>6436
                     /locus_tag="VIG70_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY8250485.1"
                     /translation="MKKVLGGGLLGLAMASALAQEDAVVITATRAPRPSLEVPASVDR
                     LYADEIREGRPQVNLSESLGRVPGIVVQNRQNYAQD"
BASE COUNT         1120 a         2098 c         2136 g         1082 t
ORIGIN      
        1 acgatgccgg tgttctacgg ctggatgctc gaccacggcg ttccgcacgc cgtgttctac
       61 acggtggccg cctttacgac gatcgcgatg ctgacggtac tgcagcttcc cgggaggtcg
      121 ggaagagtcg ccgcgcacta gtccgcgtag cgaatgcctc ttctgtccaa catccgcagc
      181 agcgcgggcc agtccttgta gtttgccgga cggtcctcgc gattcaacgt tgcggctgcc
      241 gccttcgccg cctgctccga catgagctga acgttgtgca cgccgcctgc gcaggcgtcg
      301 acctggatct ggcacgcggc gtcgaggtag tacatcactt cgaaagcgcc gcgcaccgtc
      361 tcgcccagcg cgagcagccc gtggttgcgc aggatcatcg ccttggaagt cttgccgagg
      421 tccgccgcca tgcgcgcctg ctcgtccttg tagagcgcga cgccctcgta gtcgtgatag
      481 gtgatgtccg cgtgcaccag catggcgtgc tggctgatcg gcagcaggcc gcacttcatc
      541 gccgacaccg cgaccccggc gcgtgtgtgg gtgtggatca cgcagccgat ctccggacgc
      601 gccgcgtgca cgcagctatg gatcacgaac ccggcctggt tgacccccat cccgagcggg
      661 tcctcgatga tcttgccctc ccagtcgatc ttgaccaggt tcgacgcggt gatctcgtcg
      721 aacatcatgc cgtagggatt gatcaggaac tcctcggtgc cggggaggcg ggccgagaag
      781 tgcgtataga tgaggtcgct ccacttgtag atcgacgcga gctggtaggc ggcggcgagg
      841 tcgacgcgca tctgccactc ttccttgttg acgcgctcgc ggatcggggt ggacttgaac
      901 ttgaggttgg cggacatggc gccatgatag gctagaaaaa tgcggtgcgc cgctctgttg
      961 ctcgcgccct tcgtcgcctg cgcggcggtc gcgcagccgt cgtgcctgaa atccccttca
     1021 gagaagtacc gcgggccgat gttcgacgcc atggcccagg tcgagtcgcg catggcgccg
     1081 cgcgtactgc ccgggatgga ccaggccggc gtgaccacga tggcgctgtt cgcgcgccag
     1141 caccccaagc gcaacggcga gccggacgtg ctcgcgctga agcgccgcta ccccgaccgc
     1201 ttcgtcctcg gcacgccgaa gcgcttcgac cagcacaagg acctggacaa cggcttcgtg
     1261 cgggatacgg tcgcggagct cgacgccggg cgctatcgat tcgtcggcga gatcctgttt
     1321 gcgcacgccg acaaggcgca tggcgaccag accatgaccg gcgagcgata cgtggcgccc
     1381 gagggcaaga acgtccgccg gctaatgtcg gcgctcgaga agcgcgccgc gccggtgatg
     1441 acgcactggg aggtctacaa ctggagccgc gactggccgt cgttcgacgc gctctacgcc
     1501 gcctttcccg gcgtcacgtt catctggccg cacgccggct tcggtagcgc cgggcaggtg
     1561 gagaccgtgc tcgcgtcgcg ccccaacgta gtggtgacct tatccaagaa ggaaaaggac
     1621 ccggacagcg tcacggacaa gaacctgcag gccgagctcg gccccgccat agtggacgag
     1681 tgcggggcaa tcctcgccga ctggcggggg ctgctggaga agtaccccga ccgcttcctg
     1741 ttcgcgacgg atgcccacaa ggatcagcgc tggttgaaat acgagaaggt cgtggagcgc
     1801 tggcgcgcca ttctcggcca gctcccggac ccgctcgccg agcgcatcgc ctggcgcaac
     1861 gccgagcgcg tctacgccgc gcgcagaccc tgattctcca gatcctgccc ttcggcggcg
     1921 tccagcgcct cgagtagaag gtccggccga ttgcgcttta gctgctccga gtcagagagc
     1981 atctgacgcc acagacgcgc acgcggcatg ccgtggtaga ggccgagcat gtggcgggtg
     2041 acgtggcgca gcgagtcgac gctcttagcg tattcgtgca ttgccgcaac cacctgagcg
     2101 cgcgtcttcc cggcgtcggc gaggaaccag gggttgctgt aggcagctct gccgatcatc
     2161 actccatcca cgctcttcag gtgaaggtcg atttgcggca gcgacgtgat tcccccattg
     2221 atcacaatct ccaggctcgg gaagtcgcgc ttgagccgat acacgtagtc gtacttgagc
     2281 ggagggatct cgcggttctc cttcggcgag aggcccttca gcacggcgtt gcgcgcgtgg
     2341 acgatgaagg tcttgcagcc ggcttgtgcc acggtgccga cgaaatcgcg gacaaacgag
     2401 tagtcctcaa ccctatcgat tccgacgcgg tgctttacgg tgaccggctt ggacaccaca
     2461 tcgcgaattc gttggacgca tgaagcgacc aacgcgggct cggccatgag gcacgccccg
     2521 aacgcgccgc gctgcacacg ttcgctcggg cagccgatgt tcaagttgat ttcgtcgtag
     2581 ccgtattgct cgccgagctt cgcgcagcgc gcgaggtcgt cgggctcgct accgccgagc
     2641 tgcagcgcaa ccggatgctc ttcctcgctg aactccaggt gccggcgcac gtcgccgtgg
     2701 atcagcgcgc cgctggtgat catctcggta tacaggccgg cgcgagagtt gacctggcgc
     2761 aggaaaaacc ggcaatgccg gtccgtcacg tccatcatcg gcgcaaccga aatgcgctta
     2821 tcgggcacgc gtcagggcga gccagatccc gccgccgatc agaaatcctc cgctcaggcg
     2881 cgacaccagc cggatgcgct tcttcgagag gaacgcaccg gcgcggccgg tgagcaccgc
     2941 gtaggcgcca tccgacacca gcgcgaccgc catcgcggtc acgccgagca ggacgatctg
     3001 cgccacgtag ttgccgctgg ggtcgacgaa ctgcgggatg aaggcgccga accagagcag
     3061 ggccttcggg ttcgccatca gcaccaggaa gccctgcaga aagaagccgc cgcgtggcag
     3121 tgccgcgttt tccccctgct ctaacaaatc tggagagcgc agaagcttcc agccgatcca
     3181 gaccaggtat gcggctccgg cccagcgcag ccactcgaag agccagccca tggcggcgat
     3241 gaccgaggag aggccgacga ccaggatcgc catcatcagg gcgagcccga gctgcgtgcc
     3301 tgcaacgttc agcagcccgg cgcgcgcgcc gtgcctcagg ctgttggcga tgatcaccgt
     3361 cacggttggc cccggaatca gggcgatcac cgcgcaggcg aggacgtagg cgagatagag
     3421 ctcgagcgac atccggtcac ctcatgtcga acgcgcggcg ccgcgtgagc tccaccatgc
     3481 aggcgaggtg gtagtcgtcg ggcgtgaccc ctttcggagc gtggtcgcgg cggcgcgcgc
     3541 attcagaatc gcggtactgc cgccactcgt cgttggcctt gcggaagctg gtcacggcat
     3601 tctcgttgcc ggcgttgtga tcacgctcgc gcgcatcctg gaaatgcagc gcgtagactt
     3661 gcagcaggtc gttgtcggcg acctgcagca gcagctcgag gcagggacgg ggcttgtcgc
     3721 gcagcgcgcc gtcgcgcgtg cactgcgtca gcggcccgac gttggcgcgc ggcagccact
     3781 cgaaaaatac cgcgcgcttg tcgtcggcca gcaggcagat gaagctcagc tcggcgctcg
     3841 gcgcgccctc cagcaccacc gcgccgttgc cccgcaggat cgaggagacg aactggctgc
     3901 cgagcttgcg cgtgtagcgc tcgatctgca ggttctggtc gcgctcgaac acgatgtcct
     3961 tcgcccgggt gccctcgcgc gccgcctcgc tcttggcgta ggcgcggcag gtctcgaccg
     4021 cggggccgtt gtattgcgcc aaagcagggg tcgcaacaaa tgccagcaat agaaagaaaa
     4081 gtctcatcgg tactgccccg tcgattgcgc ccgcgcgcgg atcagcccga acaggctctc
     4141 ggtgaacggc gcgcgcacgc cggcctggcg cgcgatctcg agcgtgccgg cgagcaggcc
     4201 gtcgatctcg agcggcttgc ccgcctcgag gtcctggagc atcgaggtct tgaagcgccc
     4261 gagcgcacgc gccatgtcga tgcgcgcctc tgggtcgatg tcggcgtcga cgccgactgc
     4321 gcgtccgatc gccagcacct cgcgcatgat ctcgaccatg taggccttca cttcctggtt
     4381 ctgaatgagc tggtctgccg tggtcacggt aagggcagac accggattga agctcacgtt
     4441 gccgagcagc ttcacccaga actccttctc gatggcgtcg gtcaccacaa tctcgaagcc
     4501 cgccgcagca aacgcctcgg cgatgcgctt ggtgcgcgcg gtgttcctgc cgccgggctc
     4561 gcccagaatc agtttggagc ccatgttgtg gctgacgagg cccggctccg gcgtcgaggc
     4621 ggccaggtgg atcacgcagc ccaccacgcg ctcggccgga atggcgcgcg agagcttgcc
     4681 gccggggtcg agcgattcga ggtgcgcgtc gcgccccttg aggccgtgga aaaaccacca
     4741 gggcacgccg ttcatcgccg tgacgacgga ggtgtctttc ccgagcaacg gcgcgatcgt
     4801 ttccgccacg tgtgtaaggc tttgcgcctt caccgtgacc aatacgtagt cctgcgcgcc
     4861 gagcgtcgcc gggtcgcttt cggccttgat actgaaggat tcggtcttac ctttgctttg
     4921 cacccgcaag ccggcgcgcc ggatggcatc gagctgcgcg ccgcgtgcga cgaccgacac
     4981 ctcgtgcccg gagcgcgcga gccacgccgc catcaagccg ccgatggcgc cggcaccgta
     5041 gacgcagatt ttcatagtgc tattctaggg ttaaccggac gaaaaacagg gacagaccac
     5101 ggttttttga ggaactccaa atgtttgcgt tcatgaaaaa aatgaagacg ctgttgccga
     5161 aggaaagcac gctccccggg cgcgcgagcg agatgcgcgt gcccgcgaag catttcgtga
     5221 acggccatgc gctgaaggcg ccgttccccg agggcatgca gctcgcgcag ttcggcctgg
     5281 gctgtttctg gggcgccgag cgcgtgttct gggaaacgcc cggcgtctat tcgaccgcag
     5341 tcggctacgc gggtggcgat acaaagaacc cgacctacga agaagtctgc agcggcttca
     5401 ccaaccacac cgaggcggtg ctcgtcgtgt tcgatccgaa gatcgtgacc tacgaagagc
     5461 tcctcaaggt gttctgggag tcgcatgatc cgacgcaagg catgcgccag ggcaacgacg
     5521 ccggctcgca ataccgctcc gcggtgtata catatggtga ggcccaggcc aaagcggcag
     5581 cgcgttccaa ggaaatgtac gagaaggcgc tgaaggccaa gggctacggt cccatcacga
     5641 cagagatccg tgccgcgccg gagttctact acgccgagga ctatcaccag cagtacctcg
     5701 cgaagaatcc aggcggctac tgcggccttg gcggcaccgg cgtttcctgt cccgcgggag
     5761 ttacggcgaa agtctgatgc gcctcgacaa gtggctctgg gcggcgcgct ttttcaagac
     5821 ccggagcctt gcgcagcagg cgatcgccgc ggggcgggtc aagctcggcg acgctcgcgt
     5881 gaaagcgtcc cacttggtca agccatcgga ttccctcgct atccgcgtcg gcgattttga
     5941 atggcagatc gttgtcagcg ctctgtcaga taaacgcgga cccgcagagc aggcgaggaa
     6001 gctctaccag gagaccgaag cgagccgggc cgagcgcgag cggcgcatgg acctgcggcg
     6061 ctggggcgcc gagccggcgc ggggtctgaa aggccggccg acaaagcgcg accggcggaa
     6121 atttgaggat ttcaccagcc cgcccgacga gtcgtaggaa tcttcgccgg gctgatgtgt
     6181 tgtagtcttt ggcgaaatga agaaagttct gggaggaggg ctgctcgggc tcgcgatggc
     6241 gtcggcgctc gcgcaggaag acgccgtggt gatcaccgcg acgcgcgcgc cgcggccgag
     6301 cctcgaggtg ccggcctcgg tcgatcgcct gtacgccgac gagatccgcg agggccggcc
     6361 gcaggtcaac ctctccgaga gcctcgggcg cgtgccgggc atcgtggtgc agaaccgcca
     6421 gaactacgcg caggac
//
LOCUS       DATBSZ010000119         7586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_85616, whole genome shotgun sequence.
ACCESSION   DATBSZ010000119 DATBSZ010000000
VERSION     DATBSZ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85616"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VIG70_07770"
     CDS             <1..214
                     /locus_tag="VIG70_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250486.1"
                     /translation="VFAPVFFAGVIFATAFRESRQPDVDFGWNVAGIVLGGLSEQLSL
                     VLGFSHLLLVAVAYYLLSAALRPRTV"
     gene            211..603
                     /locus_tag="VIG70_07775"
     CDS             211..603
                     /locus_tag="VIG70_07775"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY8250487.1"
                     /translation="MNASRPLPWRFVAFVLVGGFCLAANTLVLWALTSGLGLHYLVST
                     VIAFVSITPLGFLLNKVITFRTRREYARIELPRYFTAMAASFAANLGLMYLLVSVLGM
                     WYLAASILVAVTLVVCNFVASDRWSFRV"
     gene            600..1691
                     /locus_tag="VIG70_07780"
     CDS             600..1691
                     /locus_tag="VIG70_07780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8250488.1"
                     /translation="MTRVAVVHEWLLDYAGSERVVREILEVVPEADLFVLIDRRDEEL
                     RSAIPRPARGTSFLQRLPRPHKWLRYYVPLMPLAVGGLDVSAYDLVISSSHAVAKGVA
                     TGENQLHLSYVHTPMRYAWDLRDEYLRAAGLDRGLRGWAARFTLERLRRWDVRSAEGV
                     DVFLANSAHVAARIRNSYGREAEVLYPPVDVAAFPLHAQKQDFYLTVSRLEAYKRVDL
                     LVEAFSAMRERKLVIVGGGSELGRLRAMAPPNVELTGRLPMPQVVERMQRARAFLFAG
                     IEDFGIVMAEAQACGTPLVAFGRGGAAEIVREDTGVLFQAQTADALIEAVRRFESGRF
                     SPEKCRANALRFDRARFRERFGELLRALR"
     gene            complement(1688..2272)
                     /gene="cysC"
                     /locus_tag="VIG70_07785"
     CDS             complement(1688..2272)
                     /gene="cysC"
                     /locus_tag="VIG70_07785"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY8250489.1"
                     /translation="MQHLTVGKVSGEERSSLLGQKPVTVWLTGLSGAGKSTLAVELER
                     RLMDAARPCYLLDGDNMRAGISRDLGFQPQDRHENIRRVAEIARLMNDAALIAITAFI
                     SPYRADRRMARDIVGAGRFIEVYLDAPLEICEQRDPKGLYRRARAGQIAEFTGVSAPY
                     EVPEAPTLVLATGRCGVEDCVAELYALVQERIRL"
     gene            2300..4294
                     /locus_tag="VIG70_07790"
     CDS             2300..4294
                     /locus_tag="VIG70_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250490.1"
                     /translation="MTDRLLPAGLVALLLVFLQWRSGVDSPVLIAVVSAAFAYVTFAA
                     GRLLAAPAAVRDGDPSSVWVLGILAICLATYALTLLLPVTAGGAFGVVAAIVIGAEFA
                     LRRKSFPKADRKALIGFALAVVLTAAWCNEPAGAYEVVRSQGVLPLWSDYFFQGSLIS
                     QFGDMRALGRGSIYLADNPSSFYHFASYGAAAAFARLLDQPGLPLAAAVWLPLGFLAM
                     LAGAYALGARLAGPAGGIAALAAIALLPDASNYGLRNGWFSFHWTLLAHAGATYAVGA
                     AFLSLALLERWASERSRAALTAGALVVLSAFFFRFHIFILLLPAWLATAIVCSVERSR
                     VRVGWLVVVVLAAGAAAANLAVSHLADAGFWRLGNPSAFYDFLWYVHTGHEPTAYTGV
                     YEKMTGIGEPIALALVAGIGFAIAAALGAFVLALPAAALVARRAGVLRPIDAACGYLF
                     FCWLLLMFFAPTPWHGDPSDLIHRPFVLLYAACAIWTLCFLVRIAGKNLWAVLLAGAI
                     LALPAIFATAGQMAKPKFRWSEFDAAVRVPPGLVEAAEHMRQHAAAGDVFAVAGLSAA
                     YATFDLSTQVCALSGMPAYLSRPHFEMIKDARRKQVVMARLAALQEIESLTDPAAAMQ
                     ALRALKVRWYLISGDQAPRWDPERARAAFRAGTVALYRIP"
     gene            complement(4257..4871)
                     /locus_tag="VIG70_07795"
     CDS             complement(4257..4871)
                     /locus_tag="VIG70_07795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8250491.1"
                     /translation="MKGLLEHPRIYSLFRRLVGRDTARSVYAREHLRLAPGQRVLDLG
                     CGPADILQFLPEVEYVGYDINPAYIERARRRFGHRGEFHCHGVDENLSFPAGKFDVVI
                     AHGVLHHLDDVAAGSLFRIARRALRPGGRLVTFDGCFVEGQAALARLFLVLDRGRHVR
                     SREAYERLARAEFDPVQSFVRHDLIRIPYTHLIMECGTAPPSRP"
     gene            complement(4868..5599)
                     /locus_tag="VIG70_07800"
     CDS             complement(4868..5599)
                     /locus_tag="VIG70_07800"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8250492.1"
                     /translation="MSDIGFDASRFEALAAVEQEHFWFRSRARLIVWALHRHFPRAQS
                     LLEIGCGTGNVLAAIKETGLNRVVGSEALPEGLAFAARRVPGAELLQMDARRIPFRDE
                     FDVVAAFDVIEHIEEDEQVLGGMFAACRSGGGILVTVPQHAWLWSERDEHARHRRRYA
                     RRDLLRKLAAAGFQRPWARSFVSLLLPLMVLSRWRKRSASEELEVGRATNRALGAVMG
                     LERALIGAGVSFPAGGSLLALAHKP"
     gene            complement(5596..6267)
                     /locus_tag="VIG70_07805"
     CDS             complement(5596..6267)
                     /locus_tag="VIG70_07805"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HEY8250493.1"
                     /translation="MKVCILQSNFLPWRGYFDLMRESGLFIVYDDAQYTKNDWRNRNR
                     IKTPRGAQWITVPVHRRLRQSIEETPIDYTRPWAEKMLNQIRESYRDAPHFEPYFSEL
                     KDLLERPASSISELNLRTIAWACRHLGIDTPVRLSREYRSQGVKTERLIGILRQAQAT
                     SYLTGPAAQSYLVPEMFEQAGIRLEYKQYRYPEYGQLYPPYDPAVSVIDLFFMQGEAA
                     RDCFG"
     gene            6283..7248
                     /locus_tag="VIG70_07810"
     CDS             6283..7248
                     /locus_tag="VIG70_07810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8250494.1"
                     /translation="MRPLGPDYSVVVPVYNAEASLEELVRRVGAVFRELGRSHEIVLV
                     DDGSKDGSWALIERLRAAGAPIRAFQFARNHGQHYALKCGLDHCSGRYAITMDDDLQH
                     PPEEIAKLVRAIEEDPAVDVVTGHYHQKEHPALRNLGAAALHRIHHAIFRTDQRISIS
                     AFRVMRRSVIDEIRHARHAQPRIGPIILSITSRIKSVPVRHEPRRHGRSGYTLRRLVG
                     DTLSSIMNYSALPLRVVSVVGLGASFIAVVLACVYLWVYFSRGIGVSGFATLVLLILF
                     TSGAVLFSFGLVGEYLRRIVAQQLMFSQYHVRAELPAEEPDPSRR"
     gene            complement(7197..>7586)
                     /locus_tag="VIG70_07815"
     CDS             complement(7197..>7586)
                     /locus_tag="VIG70_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250495.1"
                     /translation="PAAAPGAIESLRDQEGREWESNSSFAPARGVSRDSVGRYAEHLP
                     AAWLAAVEWLCAPEMKVLGLDPVGGQAMEGVQDVVGGDELDVERQRIRVIGDPHAPEA
                     EQRRWCIFPGAYRRLGSGSSAGSSART"
BASE COUNT         1055 a         2650 c         2573 g         1308 t
ORIGIN      
        1 ggtgttcgcg ccggtgttct tcgccggcgt gatcttcgcc accgccttcc gcgagagccg
       61 gcagccggac gtcgatttcg gctggaacgt ggccggcatc gtcctcggcg gcctgagcga
      121 gcagctgtcg ctcgtgctcg ggttcagcca tctcctgctg gtggcagtgg cctattacct
      181 gctgtcggcc gccttgcggc cgcgcaccgt ttgaacgcaa gccgccccct tccctggcgt
      241 ttcgtcgcgt tcgtcctggt cggcggcttc tgcctggcgg cgaacacgct cgtcctctgg
      301 gcgctcactt cggggctcgg cctccactac ctcgtctcga cggtgatcgc cttcgtctcg
      361 atcacgccgc tcggcttcct gctgaacaag gtgatcactt tccgcacccg gcgcgagtac
      421 gcgcgcatcg agctaccgcg ctatttcacc gcgatggccg caagcttcgc ggcgaacctc
      481 gggctcatgt acctgctcgt gtcggtgctc ggcatgtggt atctcgcggc cagcatcctg
      541 gtcgcggtga cgctcgtggt ctgcaacttc gtcgcaagcg accgctggag cttccgggta
      601 tgacccgcgt cgccgtggtt catgagtggc tgctcgacta cgccggcagc gagcgcgtgg
      661 tgcgcgagat cctcgaggtc gtgcccgagg cggacctctt cgtcctcatc gaccggcgcg
      721 acgaggagct gaggagcgcg atcccgcgcc ccgcccgcgg cacgagcttc ctgcagcggc
      781 tgccgcgccc gcacaagtgg ctgcgctact acgtgccgct gatgcccctc gccgtcgggg
      841 ggctcgacgt ttcggcctac gacctcgtga tctcgagcag ccacgccgtg gccaaggggg
      901 tcgcgaccgg agaaaaccag ctgcacttga gctacgtcca taccccgatg cgttatgcct
      961 gggacctgcg cgacgagtat ctgcgcgccg ccgggctgga ccgcggcctg cgcggctggg
     1021 ccgcgcgctt caccctcgag cggctgcggc gctgggacgt gcgcagcgcc gagggcgtcg
     1081 acgtgtttct cgccaactca gcccatgtcg ccgcgcgcat ccgcaactcc tacggccggg
     1141 aggccgaggt gctctaccca ccggtggacg tcgcggcttt tccgctgcac gcgcaaaagc
     1201 aggacttcta tctcaccgtc tcgcgcctcg aggcgtacaa gcgcgtcgac ttgctcgtgg
     1261 aagccttctc tgccatgaga gagcggaagc tggtcatcgt cggcggcggg agcgagctcg
     1321 gccggctgcg cgccatggca ccgccgaacg tggagctcac cggacgcttg ccgatgccgc
     1381 aggtggtcga gcgcatgcag cgggcgcggg cgttcctctt cgccgggatc gaggatttcg
     1441 gcatcgtgat ggcggaagcc caggcctgcg gcacgccgct ggtcgccttc ggccgcggcg
     1501 gcgcggccga gatcgtgcgc gaggataccg gagtgctgtt tcaggcgcag accgcggacg
     1561 cgctgatcga ggccgtgcgc cgcttcgagt cgggtcgctt ttcacccgag aaatgcaggg
     1621 cgaacgcctt gcgcttcgat cgggcccgct tcagagagcg gttcggcgag ctgctccgcg
     1681 cgctgcgcta gaggcggatt ctttcctgca ccagcgcgta caactcggcc acgcagtctt
     1741 ccacgccgca gcggccggtg gcgaggacga gcgtcggcgc ctcgggcacc tcgtagggag
     1801 cgctgacgcc ggtgaactcg gcgatctggc ccgcccgcgc cctgcggtac aaccctttag
     1861 gatcgcgctg ttcgcagatc tcgagcgggg catccagata gacctcgata aagcgccccg
     1921 cgccgacgat gtcgcgggcc atgcggcggt cggcgcgata gggagagatg aacgccgtaa
     1981 tggcgatcag cgcggcgtcg ttcatcaaac gcgcgatttc ggcgacgcgc cggatgttct
     2041 cgtgccggtc ctgcggctgg aacccgaggt cgcggctgat gccggcgcgc atgttgtcgc
     2101 cgtcgagcag gtagcagggc ctcgcggcat ccatcagccg ccgctcgagc tcgaccgcga
     2161 gggtggactt gccggcgccg gagagcccgg tcagccagac ggtgaccggc ttctgcccga
     2221 ggaggctcga gcgctcctcg cccgatacct tgccgaccgt caagtgctgc atcgcggcaa
     2281 tgatattcgg taacgcgccg tgacggatag gctgctgccg gcgggcctgg tcgctctgct
     2341 gctcgtcttc ctgcagtggc gcagcggcgt cgattcccct gtgctcatcg ccgtggtctc
     2401 ggcggcattc gcctacgtca cgttcgccgc cggacgcctg ctggccgcac ccgccgcggt
     2461 gcgcgacggc gatccctcgt cggtctgggt cctcggcatc ctcgccattt gcctcgcgac
     2521 ctacgcactc accctgctcc tgccggtcac cgcgggcggg gcattcggcg tcgtcgcggc
     2581 catcgtgatc ggggccgagt tcgccctgcg gcgcaagtcc ttccccaaag cagaccgaaa
     2641 agcgttgatt ggattcgcgc tcgccgtcgt gctgacggcg gcgtggtgca acgagcccgc
     2701 gggcgcctac gaggtcgtgc gcagccaagg cgtgctgccg ctgtggagcg actatttctt
     2761 ccagggcagc ctcatctccc agttcgggga catgcgcgcg cttggccgcg gctcgatcta
     2821 cctcgccgac aacccgtcgt ccttctatca cttcgcgtcc tacggcgcgg ctgccgcctt
     2881 tgcgcgcttg ctcgaccagc ccggcttgcc gctcgctgcg gcggtatggc tgcctctcgg
     2941 ctttctcgcc atgctggctg gcgcctacgc gcttggggcg cggcttgccg gacccgccgg
     3001 cggcatcgcg gcgctcgccg cgattgcgct attgccggac gcgtccaact acggcctgcg
     3061 caacggctgg ttcagctttc actggacgct gctggcgcac gcaggggcga cctacgcggt
     3121 cggcgcggcg ttcctctcgc tcgcgctcct cgagcgctgg gcgagcgagc gctcgcgcgc
     3181 cgcgctgacg gccggcgcgc tcgtcgttct gtcggcgttc ttcttccgct tccacatctt
     3241 catcctcctc ctgccggcct ggctcgccac cgcgatcgtg tgcagcgtgg agagatcgag
     3301 ggtccgggtc ggctggctgg tggtcgtcgt gctcgcagca ggcgccgcgg cagcgaacct
     3361 tgcggtctcg cacctcgccg acgccggctt ctggcggctg ggcaacccct cggcgttcta
     3421 cgacttcctg tggtacgtgc acaccgggca cgagccgacc gcctatacgg gcgtctacga
     3481 gaagatgacc ggcatcggcg agccgattgc gctcgcgctc gttgccggga tcggattcgc
     3541 gatcgccgcc gcgctcggcg cgttcgttct ggccctccct gccgcggccc tcgtcgcgcg
     3601 cagggccggg gttctgcggc cgatcgacgc ggcgtgcggc tatctcttct tctgctggct
     3661 cctcctgatg ttcttcgcgc ccacgccgtg gcacggcgac ccgagcgacc tcatccatcg
     3721 gccgttcgtg ctgctgtacg cggcctgcgc catctggacg ctttgttttc tggtacggat
     3781 tgctggaaag aacctgtggg ccgtactgct cgccggcgcg atcctcgcgt tgcctgcgat
     3841 cttcgcgacc gccggccaga tggcgaagcc gaagttccgc tggagcgagt tcgacgccgc
     3901 ggtgcgcgtc ccgcccgggc tggtcgaggc tgcggagcac atgcgccagc atgcggccgc
     3961 cggcgacgtc ttcgccgtgg cggggctgtc cgcggcctac gcgaccttcg acctttccac
     4021 ccaggtctgc gccctgagcg gcatgcccgc ctacctgtcg cgcccccact tcgagatgat
     4081 caaggatgcg cggcgcaagc aggtggtcat ggcccgcctt gccgccctgc aggagatcga
     4141 atcgttgact gaccctgctg cggcgatgca ggcgctgcgc gccctgaagg tccggtggta
     4201 cttgatctcg ggcgaccagg cgccgcgctg ggatccggag cgcgcccgcg ccgccttcag
     4261 ggccgggacg gtggcgctgt accgcattcc atgatcaggt gcgtgtaggg aatgcggatc
     4321 aggtcgtggc gcacgaagct ctgcaccggg tcgaactcgg cgcgggcgag ccgctcgtag
     4381 gcttcccggc tgcgcacatg ccggccgcgg tcgagcacta ggaaaaggcg cgcgagcgcg
     4441 gcctggcctt cgacgaagca gccgtcgaag gtcacgagac gcccgcccgg cctgagcgcg
     4501 cgccgcgcga tgcggaacag cgagcccgcg gctacgtcgt ccaggtgatg caggacgccg
     4561 tgcgcaatga cgacgtcgaa ttttcctgcg gggaaggaca gattctcgtc taccccatgg
     4621 caatggaact cgccccgatg gccgaaacgg cgccgcgcgc gctcgatata ggcgggattg
     4681 atgtcgtagc ccacgtattc gacttccggc aggaactgga ggatgtcggc cgggccgcag
     4741 ccgagatcga gcactcgctg gcccggcgcg aggcgcaggt gctcgcgcgc atagaccgag
     4801 cgcgccgtat cgcggccgac cagccggcga aacaggctgt agatgcgcgg gtgctcgagc
     4861 agccctttca tggcttgtgg gcgagggcga ggagcgagcc gccggcgggg aaagaaacgc
     4921 ctgcgccgat gagcgcccgc tcgagcccca tgacggcgcc gagcgcgcga ttcgtcgcgc
     4981 ggccgacctc gagctcctcc gatgcagagc gcttgcgcca ccgcgacagg accatcagcg
     5041 gcagcagcag gctgacgaac gacctcgccc atgggcgctg gaagcccgcg gcggccagct
     5101 tgcgaagcag gtcgcggcgc gcgtagcgcc gccggtgccg ggcgtgctcg tcgcgctcgc
     5161 tccacagcca cgcgtgctgc ggcaccgtga cgaggatgcc gccgccggag cggcaggcgg
     5221 cgaacatccc gcccagcacc tgctcgtctt cctctatatg ctcgatgacg tcgaaggcgg
     5281 cgacgacgtc gaactcgtcg cggaagggga tgcggcgcgc gtccatctgc aggagctccg
     5341 cgcccggcac ccggcgcgcc gcgaaggcga ggccctcggg aagcgcctcc gagccaacca
     5401 ctcgattcaa tcctgtttcc ttgattgccg ccagaacgtt tccggtgccg cagccgattt
     5461 cgagcaggct ctgcgcgcgc ggaaaatgcc tgtgcagcgc ccagacgatg agccgggcgc
     5521 gcgagcggaa ccagaagtgc tcctgctcga cggcggcgag cgcctcgaag cgcgacgcgt
     5581 cgaagccgat gtcgctcatc cgaagcagtc tcgggcggct tcgccctgca tgaaaaagag
     5641 gtcgatcacg ctcaccgcgg ggtcgtacgg cgggtagagc tgcccgtatt ccggatagcg
     5701 gtattgcttg tactccagcc ggattcccgc ctgctcgaac atctccggga ccaggtagct
     5761 ctgcgccgcg ggcccggtga gatagctcgt ggcctgcgcc tgccggagaa ttccgatcag
     5821 cctttcggtc ttcacgccct gcgagcggta ctcgcgcgac aggcgcaccg gcgtgtcgat
     5881 gccgaggtgg cggcaggccc aggcgatggt gcgcaggttg agctcggaga tcgaggacgc
     5941 cggacgctcg aggaggtcct tcagctcgga aaaatacggc tcgaagtgcg gcgcgtcccg
     6001 gtacgactcg cggatctggt tcagcatctt ctccgcccag ggacgcgtgt aatcgatggg
     6061 cgtctcttcg atcgactggc gcagacgccg atgcaccggt acagtgatcc attgcgcgcc
     6121 gcgcggcgtc ttgatgcggt tgcggttgcg ccagtcgttt tttgtatatt gcgcgtcgtc
     6181 gtagacgatg aacaggccgg attcgcgcat cagatcgaaa tagccgcgcc aggggaggaa
     6241 gttcgactgc aggatgcaca ccttcatgtg cggctagcat agatgaggcc gctcggtccc
     6301 gactactcgg tagtggtccc ggtctacaac gccgaagcca gcctggaaga gctggtgcgg
     6361 cgcgtcggcg cggtgttccg cgagcttggc cgctcgcacg agatcgtgct ggtggacgac
     6421 ggcagcaagg acgggtcctg ggccctgatc gagcgcttgc gcgccgcggg cgccccgatc
     6481 cgtgctttcc agttcgcgcg taaccacggc cagcattacg cgctgaaatg cggcctcgac
     6541 cactgctcgg ggcgctacgc catcaccatg gacgacgatc tccagcaccc gccggaggag
     6601 atcgccaagc tggtgcgggc gatcgaagag gatccggccg tcgacgtggt gaccgggcac
     6661 taccaccaga aggagcaccc ggcgctgcgc aacctgggcg ccgcggcgct gcaccgcatc
     6721 caccacgcca ttttccgcac cgaccagcgc atctcgatca gcgcattccg cgtcatgcgc
     6781 cgcagcgtga tcgacgagat ccgccacgcc cggcacgccc agccgcgcat cggcccgatc
     6841 atcctgtcga tcaccagccg catcaagagc gtcccggtgc gccacgagcc gcgccgccac
     6901 ggccgctcgg gctacaccct gcgccggctg gtcggcgaca cgctgagcag catcatgaac
     6961 tacagcgcgc tgccgctacg cgtggtgagc gtcgtcggcc tgggggcttc cttcatcgcg
     7021 gtggtgctcg cctgcgtgta cctgtgggtc tatttcagcc gcggcatcgg agtatcgggc
     7081 ttcgcgacgc tggtcctgtt gatcctcttc acctccgggg cggtgctgtt ctcctttggg
     7141 ctggtgggcg aatacctgcg ccgaatcgtg gcccagcaac tcatgttttc ccaatatcac
     7201 gtccgcgccg agctcccggc cgaggaaccc gatccgagcc gccggtaggc gcccgggaag
     7261 atgcaccagc ggcgctgctc ggcctcgggc gcgtgagggt ccccgatgac ccggatccgc
     7321 tggcgctcga cgtcgagctc gtcgccaccg acgacatcct gcacaccctc catcgcctgg
     7381 ccgccgacgg gatcgaggcc gagcaccttc atctcgggag cgcacagcca ttccaccgcc
     7441 gccagccacg cggccggcag gtgctcggcg tatcgcccca cggagtcgcg actgacgccg
     7501 cgcgccggcg cgaacgacga gttgctctcc cattcgcgcc cttcctggtc gcgcaggctt
     7561 tcgatcgcgc ccggcgccgc ggccgg
//
LOCUS       DATBSZ010000120         5622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1792651, whole genome shotgun sequence.
ACCESSION   DATBSZ010000120 DATBSZ010000000
VERSION     DATBSZ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792651"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VIG70_07820"
     CDS             complement(<1..221)
                     /locus_tag="VIG70_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250496.1"
                     /translation="MNSSLLLRLLALVAVALFASAARAQSETKESAPPPQPEAEKATT
                     GTATPTVYKPPLRGAPATRVGGGTRNTGS"
     gene            complement(316..2508)
                     /locus_tag="VIG70_07825"
     CDS             complement(316..2508)
                     /locus_tag="VIG70_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY8250497.1"
                     /translation="MQAGLQSFHAGAFAEALGHWNAAASAYQASGNSEGALTALMRAA
                     EADLALGRNPDALAKLSQAQALAQRLGNQSLLAAVENSLGNAYLLGGRRTEAEPLLRS
                     SVERARKSKNMQTAASALNNLGNLRAAEGRFDDAGLLYREAQQSAEAVGNKTLALRAA
                     ANRARSLVDARRYGDAASALRALDGDMRALEQTNDKAVGLISIGRLYARILAQGLSPA
                     GEWSRRSYAALTDGLAVAEVINDARARSYALGYLGELYEQGARREEALQFTQRAIVAA
                     QQADAPDILFRWQWQAGRLLRSQGDGEQAMLSYQQAVATLATVRQDLTAGGARTSFRE
                     SIGPVYSELADLLLERSASLTDPKLAAQHLVDARSTMELFKGAELQDYFQDDCVTALR
                     ARTTGIDQLAPRTAALYPIILKDRLELLLTLPDGIKRFTTRVPADALTGEIRAFRLLL
                     EKRTTHQYYPHARKLYDWLIAPIAAELDKQQVDTLVIVPDGALRTIPLAALHDGEQFL
                     IARYALVTTPGLTLTDPRPLPRERAQLLLNGLTESVQGFAPLPYVAEELQAIHKIYGG
                     TMLQNGQFTLSNMEKGLEQTPYSIVHIASHGQFDSDSEKTFLLTYEGKLSMDKLEQFL
                     GLSKFRTEAVELLTLSACQTAAGDDRAALGLAGVAIKAGARSALATLWTVNDPASALM
                     VSDFYRHLRAPGVSKAKALQQAQLGILKDIRYRHPNYWSAFLLIGNWL"
     gene            2635..4563
                     /locus_tag="VIG70_07830"
     CDS             2635..4563
                     /locus_tag="VIG70_07830"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8250498.1"
                     /translation="MRPRLHSPLLAAALASALAFAAVCGLRWGGALEALELAFYDWHI
                     RHRPAGTAPAPRVVLVNITEKDIQELGTWPLPDALLAEVLETVSDAGARAIGLDVYRD
                     TPVPPGHERLNAVLSKHSQIIAPTLLPQDGKPGVPPPGALKESDRIGFTDVVVDPGGT
                     VRRGLLLMDDGQRVFYSLPLRLALLYLQAEGVALGGDPENPSHLRLGRTTFRPLEPND
                     GSYVRADAGGYQFLLDYRDPPDVFPAVTLSELRAGQFGPALFKDKVVLVGVTAESVKD
                     SFYTPYGQTFGEKKAMYGVVLIGHTVSQLLRAGLEGDAPTAVISDTNEVLWILLWSLL
                     GGAAALLLRSAWRFVLVVLAALALLAMAVHALFVLGVWIPLVPPALAWVGAAGLGTAY
                     LLSEEKRERASLMGLFSSYMSKALADEIWEHRDDFLSGGRPRPQRLTATISFADVVNF
                     TTVSEQTEPQTLMDWFYEFMSTVTPLVSVHGGVILRFLGDSIMAAFGAPMARTSEKEI
                     SQDAANAVDCALATQAAVVELNRRLESRGWPLISMRIGILTGPVVAGSIGSAARLEYN
                     LHGDTVNTASRLESFDKVGFAPDYFSTPCRILVGEPTLRLLGDQFHTELVGEYQLKGK
                     VHPIRIHRVHGRRTGSAH"
     gene            complement(4552..5193)
                     /locus_tag="VIG70_07835"
     CDS             complement(4552..5193)
                     /locus_tag="VIG70_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250499.1"
                     /translation="MNAQPLAFVVHVGDIGSSAAACADEWLLARKAQFGRIRHRFVLL
                     PGDNEWTDCKDPLARLQRWRQIFCEIPREYCEHVRWETAGWVFVALNVPGSNNNMRHA
                     EHGPRMQAVLSSLDEAAAAAQGGGGKGLVVLMQANPFVTVPRDGYSELRTSLQRLAAQ
                     SPGRVVLIHGDTHLYRDDEPLPGLRRIEVWGSPVVSWLRVTISPDRLRVDSVR"
     gene            complement(5322..>5622)
                     /locus_tag="VIG70_07840"
     CDS             complement(5322..>5622)
                     /locus_tag="VIG70_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S oxidoreductase"
                     /protein_id="HEY8250500.1"
                     /translation="VQNVGQKTREMLESIPGTEVTTVERCSGHDGTWGVKTEYFDNSM
                     KIGRPVFRQMAEPAPAYVSSDCPIAARHIMQGMGESAGKSVKAHPLTLLRTAYGI"
BASE COUNT          852 a         1882 c         1964 g          924 t
ORIGIN      
        1 ttggagcccg tgttgcgcgt tccgccgccg acgcgggtgg cgggcgcgcc gcgcagcggc
       61 ggcttgtaga ccgtcggcgt cgccgtaccc gtagtcgcct tttccgcctc gggctgagga
      121 ggaggagccg actccttggt ctccgattgc gcgcgagccg cggaagcaaa gagcgcgacc
      181 gccaccagcg cgagcaagcg taggaggagt gagctgttca taaactgtcc ctcacgatac
      241 cggaaacata gacctgcgcc gccgctgaag cgagcggcgc aaggcgcgcg attactgtgc
      301 gaaattcccc tgtaatcaca accagttgcc gatgagcagg aacgcgctcc agtaattggg
      361 gtggcgataa cggatgtcct tgaggatgcc gagctgggcc tgctgcagcg ccttcgcctt
      421 ggaaacgccg ggagcgcgca agtggcgata gaagtccgac accatcaggg cggaggccgg
      481 atcgttcacc gtccacaggg tcgcgagcgc gctgcgcgcg ccggccttga tcgccacccc
      541 ggcgaggccc agcgctgcgc ggtcgtcgcc cgcggcggtc tggcaggcgc tcagggtgag
      601 cagctcgacc gcctcggtgc ggaacttcga caggcccagg aactgctcga gcttgtccat
      661 gctgagcttg ccctcgtagg tcaggagaaa agtcttctcg ctgtcgctgt cgaactgccc
      721 gtgcgaggcg atgtgcacga tcgagtaggg cgtctgctcg agccccttct ccatgttcga
      781 cagggtgaac tgcccgttct gcagcatcgt gccgccgtag atcttgtgga tggcctgcag
      841 ctcctcggcc acgtagggaa gcggcgcgaa gccctgcacc gactcggtga gcccattgag
      901 gagaagctgc gcgcgctcgc gcggcagcgg gcgcgggtcg gtgagggtga ggcccggggt
      961 ggtcacgagc gcatagcgcg cgatcaggaa ctgctcgccg tcgtgcagcg ccgcgagcgg
     1021 aatcgtgcgc agcgcgccgt cgggcacgat caccagcgtg tcgacctgct gcttgtcgag
     1081 ctcggccgcg atcggcgcga tcagccagtc gtagagcttg cgtgcatggg gatagtactg
     1141 gtgggtggtc ctcttctcga gcagcagccg aaacgcccgg atctcgccgg tcagcgcgtc
     1201 cgccggcacg cgcgtggtga agcgcttaat cccgtcgggc agggtgagca ggagctcgag
     1261 ccggtccttg agaatgatcg gatagagcgc ggcggtgcgc ggcgcgagct ggtcgatgcc
     1321 ggtggtgcgc gcgcgcaatg cggtgacgca gtcgtcctgg aaatagtcct gcagctcggc
     1381 gcccttgaag agctccatgg tgctgcgcgc gtcgacgagg tgctgcgcgg cgagcttcgg
     1441 gtccgtgagg ctcgccgagc gctcgagcag cagatcggcg agctccgaat acaccggccc
     1501 gatggattcg cggaacgaag tgcgtgcgcc gcccgccgtc aggtcctggc gcacggtggc
     1561 gagcgtggcc acggcctgct gataggaaag catcgcctgc tccccgtcgc cctgcgagcg
     1621 tagcaggcgc ccggcctgcc attgccagcg aaacaggatg tccggcgcgt ccgcctgctg
     1681 cgcggcgacg atcgcccgtt gcgtgaattg cagcgcttcc tcgcggcgcg cgccctgctc
     1741 gtacagctct ccgagatagc cgagcgcata ggagcgcgcg cgggcgtcgt tgatgacttc
     1801 ggcaactgcg aggccgtcgg tcagtgcggc gtacgagcgc cggctccatt cgcctgcggg
     1861 ggataatccc tgagcaagaa ttctcgcgta taggcggccg atgctgatca gcccgacggc
     1921 cttgtcgttc gtctgctcga gcgcgcgcat gtcgccgtcc aacgctcgca gggcgctggc
     1981 ggcgtcgccg tagcgcctgg cgtcgaccag gctgcgcgcg cggttggcgg ctgcgcgcag
     2041 ggcgagggtc ttgttaccca cggcctcggc ggactgctgc gcctcgcgat agagcaggcc
     2101 cgcgtcgtcg aagcgtcctt ccgcagcgcg aagattcccg aggttgttga gcgccgacgc
     2161 cgcggtctgc atgttcttcg acttgcgcgc gcgctcgacg ctcgaacgca gaagcggctc
     2221 ggcttcggtc cgccggccgc ccaggaggta ggcgttgccg agactgtttt ccacggcggc
     2281 gagcagcgat tggttgccca ggcgctgagc gagcgcctgc gcctggctga gcttggcgag
     2341 cgcgtccgga ttgcggccaa gggcgagatc ggcttcggcc gcgcgcatca gcgccgtgag
     2401 agcgccttcg gaatttccgg acgcctgata ggcgctcgcc gccgcgttcc agtgcccgag
     2461 cgcctccgcg aaggcgcccg cgtggaacga ctgcaagccc gcttgcatgc gctcatcggc
     2521 cggtgcagcc gcgtgcgctg aagccagcgc tccggcgagc gctagcagcg gtaagaaacg
     2581 gcaccagcgg ttcatggctc cccccaagga ccgacgtgct ctattctgcc acccatgcga
     2641 ccgcgactgc attctccgct gctggctgcc gcgctggcca gcgccctggc ctttgccgcg
     2701 gtctgcggcc tgcggtgggg cggagcgctg gaggccctgg agctggcctt ctacgattgg
     2761 catatccgcc accggccggc agggaccgcg ccggcaccgc gggtcgtgct ggtgaacatc
     2821 accgagaagg acatccagga gctcggcacc tggccgctgc ccgatgcgct gctcgcggag
     2881 gtcctggaaa ccgtttcgga cgcaggcgcg cgggcgatcg gcctggacgt ataccgcgac
     2941 accccggtgc cgcctggaca cgagcggttg aacgcggtgc tgagcaagca ctcgcagatc
     3001 atcgccccga cgctgctccc ccaggacggc aagccgggcg tgccgccgcc cggggcgctg
     3061 aaagagagcg accgcatcgg gttcaccgac gtcgtggtcg accccggcgg caccgtgcga
     3121 cgcggcctgc tgctgatgga cgacggccag cgggtgttct actcgctgcc gctgcggctc
     3181 gcgctgctct acctgcaggc agaaggcgtt gcgctcggcg gcgatccgga aaacccgagc
     3241 catctcaggc tcgggcgcac cacctttcgt ccgctcgagc cgaacgacgg cagctatgtg
     3301 cgcgccgacg cgggcggcta tcagttcctg ctcgactatc gcgatccacc cgacgttttt
     3361 cccgcggtca cgctgagcga gctgcgcgcc ggccagttcg gtcccgcgct gttcaaggac
     3421 aaggtggtgc tggtgggtgt caccgccgaa agcgtcaagg acagctttta cacgccctat
     3481 ggccaaactt tcggcgagaa aaaggcgatg tacggggtgg tgctcatcgg tcacacggtg
     3541 agccagctgc tgcgcgccgg cctcgagggc gatgcgccga cggcggtgat cagcgacacg
     3601 aacgaagtgc tctggatcct gttgtggagc ctgctgggcg gcgccgcggc gctcctgctg
     3661 cgctcggcat ggcgcttcgt gctggtggtg ctcgccgcgc tggcgctgct cgcgatggcg
     3721 gtgcatgcgc tgttcgtgct cggcgtgtgg attccgctcg tgccgcctgc gctcgcctgg
     3781 gtcggcgctg ccggtctagg gacggcgtat ctcctgagcg aggagaagcg ggagcgcgcg
     3841 agcctcatgg gcctgttctc gagctacatg tcgaaggcgc tggccgacga gatctgggag
     3901 caccgggacg atttcctcag cggcgggcgc ccccggccgc agcggctgac cgccacgatc
     3961 tcctttgccg acgtggtgaa cttcaccacg gtctcggagc aaaccgagcc gcagacgctg
     4021 atggactggt tctacgagtt catgtcgacc gtcaccccgc tggtgagcgt gcacggcggc
     4081 gtcatcctgc ggtttctcgg cgactcgatc atggccgcat tcggcgcgcc gatggcgcgc
     4141 accagcgaga aggagatcag ccaggatgct gcgaacgctg tcgactgcgc gctcgccacc
     4201 caggcggcgg tcgtcgagct caaccggcgg ctcgagagcc gcggatggcc gctgatcagc
     4261 atgcgcatcg gcatcctcac cggacctgtc gtggcgggga gcatcggcag cgcggcgcgc
     4321 ctggagtaca acctgcacgg cgacactgtg aacaccgcct cgcgcctcga aagcttcgac
     4381 aaggtgggct tcgcgcccga ctacttcagc accccatgcc ggatcctggt cggcgagccg
     4441 accctgcgtc tccttgggga tcaattccac accgagctgg tgggggagta ccagctcaag
     4501 ggcaaggtcc acccgatccg gatccaccgc gtgcacggac ggcgaacagg atcagcgcac
     4561 tgagtcgacg cggagtcggt cgggcgagat cgtcacgcgc agccagctca ccaccggcga
     4621 gccccagacc tcgatgcggc gcaatcccgg cagcggctcg tcgtccctgt agaggtgcgt
     4681 atcgccgtgg attaggacga cccttccagg gctttgcgcc gcgaggcgtt ggaggctcgt
     4741 ccttagctcc gaatagccgt cgcgcggaac ggtcacgaag gggttggcct gcatcaggac
     4801 gaccagtcct ttcccgcccc cgccctgcgc cgccgcggcc gcctcgtcca gcgacgacag
     4861 caccgcctgc atgcgcggcc cgtgctcggc atggcgcatg ttgttgttgc tgccgggaac
     4921 attgagcgcg acgaacaccc agcctgcggt ttcccagcgg acatgctcgc agtattcgcg
     4981 cgggatctcg cagaagatct gccgccagcg ctgcaggcgt gccagggggt ccttgcagtc
     5041 ggtccactcg ttgtcgccgg gcaggagcac gaagcggtgc cggatgcggc cgaactgcgc
     5101 ttttcgcgcg agcagccact cgtcggcgca cgccgccgcg ctcgagccga tgtcgcccac
     5161 gtgcaccacg aaggcgagcg gctgcgcgtt catctcgtcg atcagcagct cgaggcgcgc
     5221 gacctccgcc tcgctatagg gcgtatcgcc cagcgcgccg aacgcgatgt cgcctgccgg
     5281 cagcggcgcg agcggcgcgc agccggcaac cagcaggaaa gctagatgcc gtacgccgtg
     5341 cgtagaagcg tgagcggatg ggccttgacg gacttgccgg ccgactcgcc catcccctgc
     5401 atgatgtgcc gcgctgcaat cgggcaatcg gagctcacgt aagccggcgc aggttcggcc
     5461 atctggcgga acaccggccg cccgatcttc atcgagttgt cgaagtactc ggtcttcacg
     5521 ccccaggtgc cgtcgtgccc cgagcagcgc tcgaccgtgg tcacctcggt gccgggaatc
     5581 gattcgagca tctcgcgcgt cttctggccc acgttctgca cg
//
LOCUS       DATBSZ010000121         4007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1792738, whole genome shotgun sequence.
ACCESSION   DATBSZ010000121 DATBSZ010000000
VERSION     DATBSZ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792738"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..692)
                     /locus_tag="VIG70_07845"
     CDS             complement(132..692)
                     /locus_tag="VIG70_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HEY8250501.1"
                     /translation="MERKQEIQKTVAEIREIEKRDGVTRESLEKIKQRLIRLAARQDL
                     FPASDFPPPAPGGKLKSCLYRVAEDADHRFALYVNASLGGHSTPAHNHTTWAVIVGVT
                     GEELNRFYDRTAEAPRAKGEYVVKQGTGVCFMPEDLHSIHIQAPLVNFHMYGLALDQL
                     HKREYYQAAEKMWKVFPAHGDIREAR"
     gene            736..1164
                     /locus_tag="VIG70_07850"
     CDS             736..1164
                     /locus_tag="VIG70_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HEY8250502.1"
                     /translation="MALKVSKAEVWAVTIDDRPGGAAEKLETLSRAGANLEMLLARRT
                     AEQPGKGVMFVTPLKGKKAVEAAQQSGMGRPENIYSVRIEGGDKPGLGSKIAATLGGA
                     GVNFRGMSAIAIGKKFVSFIALDSADDQARAIAALKKLKV"
     gene            complement(1243..1737)
                     /locus_tag="VIG70_07855"
     CDS             complement(1243..1737)
                     /locus_tag="VIG70_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250503.1"
                     /translation="MKTLLFTAALLFALPAYAVLDIKDVVLGSKESDVKRQFPSARCK
                     ALEWESKAADRRCDDSRVSFGGVEVQVTFYLKKDAVEAFDVRFDTRELDRLVGFLKQR
                     YGAPQSEQKDSYERKGKNARQVYKALWESGKDRAVLTAQLEKRRASMLVSRGGFDEEI
                     YRVR"
     gene            complement(1749..2447)
                     /locus_tag="VIG70_07860"
     CDS             complement(1749..2447)
                     /locus_tag="VIG70_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY8250504.1"
                     /translation="MIALYELKGKNDRRYSLFSWRTRMALRHKGLDFESNPVRMSDKA
                     AIAFSGGKTVPVIKDGAGGEKEVVVRDSWKIAEYLEERYPDAPSLFGGEIGRGVTQAL
                     NTWVDRALVPAMLQVIAADIHERVDPLDEEYFRQSMEKVLKQTLEESRARRDAALVQL
                     GRVLAPMQETFKRQAFMGGARPAYADYILFSVFQWARVMSPHELLGPEDPLCRWRERM
                     LDLFDGFARNVPTA"
     gene            complement(2444..3913)
                     /locus_tag="VIG70_07865"
     CDS             complement(2444..3913)
                     /locus_tag="VIG70_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEY8250505.1"
                     /translation="MTLWELVERRSRDAPGAASVLFENQKLTNREFHDRALRVARGLA
                     GLGIGRGDVVAVQLPNIPEYLLSYAAICALGATLQPVHMPYRRAELATLLGHSEARAF
                     ICLSRLKEERPPGLKNVVSLGEPEENAVAFSKISTSPLLGRVKEGSPEDRFLLLYTSG
                     TTDNPKGVPHAQRGFLANAMASVPELEISKEEIVLSAAPLTHLYGLYAYHVSLCSGAA
                     MSLLPAFTPPDLAKVIEKHKANCVFAGPAHFKPLLDGGLLERHDFSSLRFACLSGSPV
                     PPELAVAVEKRLPKGKTIQLWGMTELQAGSFSRPSDPADVRHGTAGAATPGTELRVVD
                     ERLQVRGISLFSGYLKNEKATREAFTPEGWFETGDTAELSAAGHLRFTGRVKEIINRG
                     GVKYSPLDVEAIIDRMPGVARSAIVPYPDAVLGERACVFVQPAPDAPAATLEAIMREL
                     DRAGVAKFKWPERLELLEAMPLTPTQKVMRGRLRERLKR"
BASE COUNT          656 a         1346 c         1314 g          691 t
ORIGIN      
        1 ccaggtcgcc gatgacgagc tccaggcgcg acagcggcgc cgagcaggcg aagaacggat
       61 ggccgcggta gtggacgcct tgctcgcgga catccagcag cgcgagctcg ccgcttcccg
      121 cgaccagctc gctaacgcgc ctcgcggata tccccatggg ccgggaacac cttccacatt
      181 ttttcggcgg cctggtagta ctcgcgcttg tgcagctggt cgagcgcgag gccgtacatg
      241 tggaagttca cgagcggcgc ctggatatgg atcgagtgca ggtcctcggg catgaagcac
      301 accccggtgc cctgcttcac cacgtactcg cccttcgcgc gcggggcctc ggcggtgcgg
      361 tcgtagaacc gattcagctc ctcgccggtg acgccgacga tcaccgccca ggtggtgtgg
      421 ttgtgcgccg gggtgctgtg cccgccgagc gaggcgttca cgtagagcgc gaagcggtgg
      481 tcggcatcct cggccacgcg gtacaggcag gacttgagct ttccgccggg ggcgggcggc
      541 gggaagtcgg acgccgggaa taggtcctgg cgggccgcga ggcggatcag ccgctgcttg
      601 atcttctcga ggctctcgcg ggtcacgccg tcgcgcttct cgatctctcg gatctcggcg
      661 acggtcttct ggatctcttg tttgcgttcc ataggagtaa attatgccgc ttgccccaac
      721 cccgaggagg aaatcatggc actgaaagtc agcaaggccg aagtctgggc ggtcaccatc
      781 gacgaccgcc ccggaggcgc tgcggagaag ctcgaaaccc tgtcccgcgc gggcgcgaac
      841 ctggagatgc tgctcgcccg ccgcaccgcg gagcagcccg gcaagggcgt gatgttcgtg
      901 acgccgctga agggcaagaa ggcggtcgag gcggcgcagc aatcgggcat gggccggccg
      961 gagaacatct actcggtgcg catcgagggc ggcgacaagc ccggcctggg ctcgaagatc
     1021 gcagccacgc tcggcggcgc gggggtcaac ttccgcggca tgtcggcgat cgctatcggc
     1081 aagaagttcg tcagcttcat cgcgctcgac agcgcggatg accaggcgcg cgcgatcgcg
     1141 gcgctgaaga agctcaaggt ctaagaacag aattcggggt cagggtgcga attctgtttt
     1201 cacgtacgta aaaacgaatt cgcaccctga ccccgaattc gtctaccgca cgcggtaaat
     1261 ttcctcgtcg aagccgccgc gcgacaccag catcgacgcg cggcgcttct cgagctgggc
     1321 cgtgagcacc gcgcggtcct tgccgctctc ccagagcgcc ttgtacacct ggcgcgcgtt
     1381 ctttcctttg cgctcgtagc tgtccttctg ctctgactgc ggtgcgccgt agcgctgctt
     1441 gaggaacccc acgaggcggt cgagctcgcg ggtgtcgaag cgaacgtcga aggcctcgac
     1501 cgcgtccttc ttcaggtaga aggtgacctg gacctcgacg ccgccgaagg agacgcgcga
     1561 atcgtcgcag cgccggtcgg cggccttgct ttcccattcg agcgccttgc agcgcgcgct
     1621 cgggaactgg cgcttgacgt cgctctcctt cgagccgagc acgacgtcct tgatgtccag
     1681 gaccgcgtac gcgggcaagg cgaagaggag agctgcggtg aacaggagcg ttttcatgcg
     1741 gtcaaccgtc aggctgtggg gacattgcga gcgaacccat cgaacaagtc cagcatgcgc
     1801 tcgcgccacc ggcacagcgg gtcctcgggc ccgagcagct cgtgcgggct catcacgcgc
     1861 gcccactgga acacggagaa caggatgtag tcggcgtacg ccggccgggc gccgcccatg
     1921 aacgcctggc gcttgaacgt ctcctgcatc ggcgcgagca cccggccgag ctgcacgagc
     1981 gccgcgtcgc ggcgcgcgcg cgattcctcc agggtttgct tgaggacctt ctccatcgat
     2041 tgcctgaaat actcctcgtc gagcggatcg acgcgctcgt ggatgtcggc ggcgatcacc
     2101 tgcagcatcg ccggcaccag cgcgcggtcg acccaggtgt tgagcgcctg cgtcacgccg
     2161 cgcccgatct cgccgccgaa gagcgacggc gcgtccgggt agcgctcctc gaggtactcg
     2221 gcgatcttcc acgaatcgcg caccacgact tccttttcgc cacctgcccc gtccttgatg
     2281 accggcaccg tcttgccgcc cgagaacgcg atcgccgcct tgtccgacat ccgcaccggg
     2341 ttcgactcga agtcgagccc cttgtgcctg agcgccatgc gcgtgcgcca ggagaacagg
     2401 ctgtagcgcc ggtcgttctt ccctttcagt tcgtacagcg caatcatctc ttcaagcgct
     2461 cgcgcaatct gccgcgcatg accttctgcg tcggcgtaag cggcatcgcc tccagcagct
     2521 cgaggcgctc gggccacttg aacttggcca cgccggcgcg atcgagctcg cgcattatcg
     2581 cttccagcgt cgccgccggg gcgtccggcg ccggctgcac gaacacgcac gcgcgctcgc
     2641 ccagcacggc gtcgggatac ggcacgatcg ccgagcgcgc cacgccgggc atgcggtcga
     2701 tgatcgcctc gacgtcgagg ggcgagtact taacgccgcc gcggttgatg atctccttaa
     2761 cgcgcccggt gaagcgcaga tgaccggcgg cgctcaattc cgcggtgtcg ccggtctcga
     2821 accagccctc gggggtaaac gcttcccggg ttgccttttc gttcttcaaa taccctgaaa
     2881 aaagcgaaat cccgcggacc tgtagtcgct cgtccacgac gcgcagctcc gtccccggcg
     2941 tcgctgcgcc ggcagtgccg tggcgcacgt cggccgggtc gctcggccgc gagaacgacc
     3001 ccgcctgcag ctcggtcatt ccccagagct ggatggtttt tcctttgggg agtctcttct
     3061 caacagccac cgccagttcc ggcggtaccg gactccccga gaggcaggcg aaccgcagcg
     3121 aggaaaaatc gtgccgctcg aggagcccgc catcgagcag cggcttgaag tgcgccggac
     3181 ccgcgaacac gcagttcgcc ttgtgctttt cgatcacttt cgcaagatcc ggcggcgtga
     3241 aagcgggcag gagcgacatc gccgctccgg agcacaggct cacgtgatag gcatagagcc
     3301 cgtagagatg cgtgagtggc gccgccgaaa gcacgatttc ctctttcgag atttcgagct
     3361 ccggcaccga cgccatcgcg ttggcgagga agccgcgctg cgcatgcggg acgcccttcg
     3421 ggttgtcggt ggtacccgag gtgtagagca gcaggaaccg gtcttcgggc gagccctcct
     3481 tcacgcgacc caggagtgga ctcgttgaaa tctttgaaaa ggcaaccgcg ttttcctccg
     3541 gctcgccgag cgagacgacg ttcttcagcc cgggcgggcg ttcttccttc aagcgagaaa
     3601 ggcagatgaa cgccctcgct tccgagtgcc cgagcagcgt ggcgagctcg gcgcggcgat
     3661 acggcatgtg caccggctgc agggttgcgc cgagcgcgca gatcgccgca tagctcagca
     3721 ggtactcggg gatgttcggg agctggaccg cgaccacgtc gccgcggccg atgccgaggc
     3781 ctgccaagcc gcgtgcgacc cggagcgctc gatcgtgaaa ctcgcgattt gtcagcttct
     3841 ggttttcaaa aagcaccgag gccgcgccgg gtgcgtcacg cgagcggcgc tcgacgagct
     3901 cccacagcgt catttcacgc cgctttcttt accggcccgt ccttgacctc gtggctcgcc
     3961 ggccgcgcct tgatgcgcag gtcctgctgc tcgacgcgct cgcgcgc
//
LOCUS       DATBSZ010000122         1977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1724521, whole genome shotgun sequence.
ACCESSION   DATBSZ010000122 DATBSZ010000000
VERSION     DATBSZ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724521"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VIG70_07870"
     CDS             <1..633
                     /locus_tag="VIG70_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8250506.1"
                     /translation="TDFKAILTKIKGKAPDAVFYGGMDATGGPMIKQARELGIKATFS
                     FGDGACTEEMGKLAGAAAEGLICSQAGLPASAASKQFNDAFKAKYGDVKQYAPFFYDA
                     VGLMIAAMQKADSTDPAKYLPELHKISYNGATGKIEFDEKGDRKNAEITIFTLKGGKV
                     EPVAVVQSGKTMGYDDFIKAMTASAPAAPAMAPAAAPATPAAPAPEAPKK"
     gene            752..1756
                     /locus_tag="VIG70_07875"
     CDS             752..1756
                     /locus_tag="VIG70_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8250507.1"
                     /translation="MDTFLQQMVNGLTLGSVYAVVALGYTMVYGIIQLINFAHGEVVM
                     IGALTAYSVIVALTGAQVPLPAVVIVFIGALAAIPVCMAIGYTLERTAYRPLRRAPRL
                     APLITAIGLSIILQHLAMIVWSRNPLSYPQIIHTVPIHLTDNPDGATITNVQVAIIVT
                     SIAMMAGLVALVYRTKLGVAMRATAQNPSIAGLMGIDINRVIALTFVIGAGLGAVAGV
                     MVGSYYGIAHYQMGALLGLKAFSAAVLGGIGNLAGAMLGGILLGIIEALGAGYVGELT
                     NLCQLSSWFDALAERCASDPNVTLFGSNYQDVFAFLVLILVLIFRPSGLLGERVSDRS
                     "
     gene            1783..>1977
                     /locus_tag="VIG70_07880"
     CDS             1783..>1977
                     /locus_tag="VIG70_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY8250508.1"
                     /translation="MLAVIAMLAAAPALAKEPALGFYVGGGVGQSNFRSDYASQVNHA
                     YEGTGFTVDVANVTDNKDTAW"
BASE COUNT          321 a          685 c          663 g          308 t
ORIGIN      
        1 accgacttca aggcgatcct gaccaagatc aagggcaagg cgcccgacgc ggtgttctac
       61 ggcggcatgg acgcgaccgg cggcccgatg atcaagcagg cgcgcgagct cggcatcaag
      121 gcgacgttca gcttcggcga cggcgcgtgc accgaggaaa tgggcaagct cgccggcgcg
      181 gcggccgaag ggctgatctg ctcgcaggcc ggcctgccgg cctcggcggc gagcaagcag
      241 ttcaacgacg cgttcaaggc gaagtacggc gacgtcaagc agtacgcgcc gttcttctac
      301 gacgccgtcg gcctcatgat cgccgcgatg cagaaggcgg attcgaccga cccggccaag
      361 tacctgcccg agctccacaa gatcagctac aacggcgcca cgggcaagat cgagttcgac
      421 gagaagggcg accgcaagaa cgccgagatc acgatcttca cactgaaggg cggcaaggtt
      481 gagccggtcg ccgtggttca gtctggcaag accatgggct acgacgactt catcaaggcg
      541 atgaccgcct cggcgcccgc cgcgccggcg atggcgcccg ctgcggcccc ggccacgccg
      601 gccgcgcccg cgccggaagc gccgaagaag tagacgctcg ttagctgcat caccggaacg
      661 gcgcccgcga gggcgccgtt tcgttttggt gcgctgcgtc gcaggcgcgg ggttgccctt
      721 ggcaccgccg cgccccacaa tcgcggcgcg catggacacc ttcctgcagc agatggtgaa
      781 cggcctgacg ctcggcagcg tctacgcggt cgtcgcgctg ggctacacga tggtgtacgg
      841 gatcatccag ctcatcaact tcgcgcacgg cgaggtcgtg atgatcggcg cgctgaccgc
      901 gtactcggtg atcgtggcgc tgaccggcgc gcaggtgccg ctgccggcgg tggtgatcgt
      961 gttcatcggc gcgctggccg cgatcccggt ctgcatggcg atcggctaca cgctcgagcg
     1021 gaccgcgtac cggccactgc ggcgcgcgcc gcgcctcgcg ccgctgatca ccgcgatcgg
     1081 cctgtcgatc atcctgcagc acctcgcgat gatcgtctgg agccgcaacc cgctctcgta
     1141 tccgcagatc atccacaccg tcccgatcca cctcaccgac aatcccgacg gcgcgaccat
     1201 caccaatgtg caggtcgcga tcatcgtgac gtcgatcgcg atgatggcgg gcctggtggc
     1261 gctcgtatac cggaccaagc tcggcgtggc catgcgcgcg accgcgcaga acccgtcgat
     1321 cgcaggcctg atgggcatcg acatcaaccg cgtcatcgcg ctcacgttcg tgatcggcgc
     1381 ggggctcggc gcggtcgccg gcgtgatggt cggcagctac tatggcatcg cgcactacca
     1441 gatgggcgcg ctcctcggcc tcaaggcgtt ctcggcggcg gtgctgggcg gcatcgggaa
     1501 cctcgcgggc gcgatgctgg gcggcatcct gctcggcatc atcgaggcgc tcggcgccgg
     1561 gtacgtcggc gaactcacca acctctgcca gctctcgagc tggttcgacg cgctcgccga
     1621 gcgctgcgcg agcgacccga acgtcacgct gttcggctcg aactaccagg acgtgttcgc
     1681 gttcctcgtg ctgatcctcg tgctcatctt ccgcccgtcg ggcctgctcg gcgagcgcgt
     1741 gtccgaccgc tcttgaccag gagtccttcg atgcgatgtc gcatgctcgc cgtgatcgcc
     1801 atgctcgccg ccgcccccgc actggccaag gagccggcgc tcgggttcta cgtcggcggc
     1861 ggcgtcggcc agtcgaactt caggagcgac tacgcgtcgc aggtgaacca cgcgtacgag
     1921 gggaccggct tcacggtcga cgtcgcgaac gtcacggaca acaaggacac cgcgtgg
//
LOCUS       DATBSZ010000123        40463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_905025, whole genome shotgun sequence.
ACCESSION   DATBSZ010000123 DATBSZ010000000
VERSION     DATBSZ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 40463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40463
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905025"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2381)
                     /gene="gyrB"
                     /locus_tag="VIG70_07885"
     CDS             complement(<1..2381)
                     /gene="gyrB"
                     /locus_tag="VIG70_07885"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEY8250509.1"
                     /translation="MSEHKGNGESGDYGSDAIKVLKGLEAVRKRPGMYIGDTSDGTGL
                     HHMVFEVVDNSVDEALAGFCDEIIVTIHTDNSVSVIDNGRGIPTGVKLDDTLARSAAE
                     IVMTELHAGGKFDSNSYKVSGGLHGVGVSVVNALSEWLRLTVWRERQVHQMEFRNGVA
                     VAPLKATGSTERRGTEVHFLASKETFGTIDYHYDILARRLRELSFLNNGVKIVLIDQR
                     AGKEENFAFSGGVKGFVEYMNRAKNVLHPNVFLGHGEKDGLTVEVAMQWNDSYQENVL
                     CFTNNIPQKDGGSHLTGLRAAMTRVLNKYIEENEIAKKAKVETTGDDMREGLACVLSV
                     KVPEPKFSSQTKEKLVSSEVRPVVEEIVAERLREFLDERPHDARTITVKIVEAAAARE
                     AARKAREMTRRKGVLDSMGLPGKLADCQERDPAKSELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLKGKILNVERARFEKLLASAEIATLITALGCGIGAEEYNPDKLRYHRIIVMTDA
                     DVDGSHIRTLLLTFFYRQMRELVERGHIYIAQPPLYKAKLGKEERYLKDEHELNAFML
                     KQALVDAALHPGDGREPISGEALAELAKSYLLSEAVIERVSRLVDREVLQAMLRGVQV
                     DLSRRDTAEASAEALEAALAPQGFAVAPHFDQKTERHQVRVERTRHGNVRLSTIDTEF
                     VLSGDYAQIRSTAEMLRGLVGAGASVQRGDKRQPVRDFAEAMKWLLGEVERSMTLQRY
                     KGLGEMNPAQLWETTMDPASRRLLRVQIEDGIAADEIFTKLMGDEVEPRRAFI"
     gene            complement(2389..3495)
                     /gene="dnaN"
                     /locus_tag="VIG70_07890"
     CDS             complement(2389..3495)
                     /gene="dnaN"
                     /locus_tag="VIG70_07890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY8250510.1"
                     /translation="MNLAKAKRDELLGPLAAVSGIIERRHTLPILSNVLIERAQDMLA
                     FLATDIEIQIAARSGIGAPADVRAVTVGARKLLDILRALPESAEVTLQQQDKRLLVKA
                     GKSRFTLQTLAAEDFPRLARPAGESARFELEQKALRRLLSLVQYAMAQQDIRYYLNGL
                     LMVVEEGSLKLVATDGHRLAFAALKLGAKLPRQEVIVPRKTVLELGKLLADSDAPVKI
                     EIAATQASFSFGDIDLVSKLIDGKFPDYTRVIPTGHKNRLQIEREPLRQALLRAAILS
                     NEKFRGVRWVLADGSLKIVSSNAEQEEAHEELEVKYSGDALDIGFNVNYLLDVLNNVS
                     GDVIECAFGDSASSALITYGAEKDFKYVVMPMRI"
     gene            complement(3694..5046)
                     /gene="dnaA"
                     /locus_tag="VIG70_07895"
     CDS             complement(3694..5046)
                     /gene="dnaA"
                     /locus_tag="VIG70_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY8250511.1"
                     /translation="MQNLWDACLRRLEQELPAQQFNTWIRPLVPESGEATDTAAHTLV
                     LTAPNRFVLELVRERFAARIERLAAEASGRELGLRLVLARVAEAPARLAPAPGDAHRR
                     PAETPSIFERARLNPAFSFDSFVTGKANQLARAAAMQVSENPGVSYNPLFVYGGVGLG
                     KTHLVQAIGNQLADQRPQARIRYIHAEAYVTDVVRAYQQKSFDEFKRYYHSLDLLLID
                     DIQFFSNKGRTQEEFFYAFNALVEAHKQIVITCDTYPKEIAGMEERLISRFGWGLTVA
                     IEPPELEMRVAIVLKKAEAESVALSEDIAFFIAKHLRSNVRELEGALKKVLAFARFSG
                     RELSLELAKEALQDILNVASRQVSVEGIQKTVAEYFKIKISDMHSKKRSRNVARPRQV
                     AMALAKDLTQMSLPEIGEAFGNRDHTTVLHACRTIASLRKNDTGLNRDYVVLEQVLKG
                     "
     gene            5168..5308
                     /gene="rpmH"
                     /locus_tag="VIG70_07900"
     CDS             5168..5308
                     /gene="rpmH"
                     /locus_tag="VIG70_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY8250512.1"
                     /translation="MATKRTYQPSKTRRARRHGFLVRSRTPGGRKVLRNRRARGRRRL
                     GL"
     gene            5350..5655
                     /gene="rnpA"
                     /locus_tag="VIG70_07905"
     CDS             5350..5655
                     /gene="rnpA"
                     /locus_tag="VIG70_07905"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY8250513.1"
                     /translation="MRQKAEFETLLRHGARRSLDGYTFYVARRETGSARLGMLISRKH
                     AARAVTRNGLKRCIREAFRLERERLGSLDVLVRPPYGAKPSAEMILRLRDIFGRLNS"
     gene            5652..5864
                     /gene="yidD"
                     /locus_tag="VIG70_07910"
     CDS             5652..5864
                     /gene="yidD"
                     /locus_tag="VIG70_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY8250514.1"
                     /translation="MIVRALRFALQGYRWCVRPLLPPACRFYPSCSHYAEEALVRHGA
                     WRGGWLAAWRVCRCGPWSPGGHDPVP"
     gene            5870..7534
                     /gene="yidC"
                     /locus_tag="VIG70_07915"
     CDS             5870..7534
                     /gene="yidC"
                     /locus_tag="VIG70_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY8250515.1"
                     /translation="MDTQRLILLVIFSFSLLMLWEAWEKEKRPKPAPQAPAAQQGVPV
                     PGKPAPPAAQAPGVAPKPAPAAVPAAAAAAKGEIVRVRTDLLVAEIDTLGGTLKRLEL
                     LKHKDSADPTKNFVLFGPDHQYEAQSGITREGGPNHLTLWTAGAKDVALEPGKDAVEV
                     RLTGASRDGVSVEKVYLFKRDSYVVDLSLEVQNRSSESLTPYAYFQLTHDGKPTSNVN
                     TVAETFGAQSFTGFALYTPEKAFEKVHSSDLDKAKFERRADNGWLGFVQHYFVSAWIP
                     PDKVARDYGALKRNDGLYSGNVVLQPGTVAPGASAQVKVSLYAGPQEQRRLQAAAPGL
                     DLVVDYGWLAIIAWPLFWLLEKFHALSGNWGVAIILLTVLIKIVFFPLSAASYKSMAK
                     MKLITPRLTKLREMYGNDRQKMNQAMMELYKTEKINPLGGCFPILVQIPVFIALYWVL
                     LAAIELRHAPFMLWIKDLSALDPYYVLPILMTMTMILQTKMNPVPPDPVQARVMQIMP
                     FVFSIFFFFFPAGLVLYWLVNNILSIAQQWQIQRMFDRDRPAHAKR"
     gene            7536..8843
                     /gene="mnmE"
                     /locus_tag="VIG70_07920"
     CDS             7536..8843
                     /gene="mnmE"
                     /locus_tag="VIG70_07920"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY8250516.1"
                     /translation="MIRDTIAAIATPTGRGGIGVVRISGPDVAGICQKLLGKLPPPRH
                     ATHAVFRDAQGEAIDEGIALYFPAPHSYTGEPVLELQGHGGPMVMQAVLAACLDAGAR
                     LAEPGEFTRRAFLEGKLDLAQAEAVADLIDAASREAARSALRSLTGEFSAAIGAMQSQ
                     LIELRALTEAMLDFPEEDVDAFHRGDAAGRLAAVRGALDDVLAKSRQGSLLRSGIHVV
                     LAGRPNVGKSSLLNRLAGEERAIVTPVAGTTRDALREPIQIEGVALVLVDTAGLRLSS
                     DMVERLGVERTQRELERADVVMAVFEAGKGRDALENLPSGVPLIEVYNKIDLVPGFAA
                     PAGAVAVSARTGEGLAELRKAILEAAGWSSSGEPVFLARERHLRALERARAHLEAATR
                     ETGRWELFAEELRLAHTALGSVVGEFSADDLLGEIFARFCIGK"
     gene            9011..10858
                     /locus_tag="VIG70_07925"
     CDS             9011..10858
                     /locus_tag="VIG70_07925"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEY8250517.1"
                     /translation="MNAPGSTLIDQSNAPSWVRHRGLRQWVTDVARLAKPERIVWCDG
                     SQAEYDRLCAELVASGTFIRLSEKLRPNCYLARSHPSDVARMEDRTFICSEEKEDAGA
                     TNNWVDPREMRATLNRLFDGCMRGRTMYVMPFSMGPIGSPIAQVGVEISDSAYVAVNM
                     RLMTRMGRAVADWLGEDGAFVPCVHSVGAPLTAGKKDVAWPCNEKEKWIVHFPETREI
                     WSYGSGYGGNALLGKKCLALRIASVMARDEGWLAEHMLILGVQAPTGEKTYVAAAFPS
                     ACGKTNLAMLIPPAAFADWKVTTIGEDIAWIKPGADGRLRAINPEYGLFGVAPGTSEQ
                     TNPNAMKMLHSNVIFTNVAVTPDGDVWWEGMTDKPPAGLTDWQGKPWTPEDGKKGRLA
                     AHANARFTVPASQCPSMDAAWEDPAGVPISAFVFGGRRSTTVPLVVEAPSWEEGIYMA
                     ATLGSETTAAITGKLGVVRRDPMAMLAFCGYNMGDYFAHWLKMGRSLKQPPKIFRVNW
                     FRRDANGKFLWPGFSENMRVLAWIVARCQGRAQGADTALGLMPRYQDLNWAGLEKMTE
                     ARFAELSQVDAAAWKEELRSHDELFAKLGARLPPALEARRGRMHEKLAA"
     gene            complement(10847..12022)
                     /locus_tag="VIG70_07930"
     CDS             complement(10847..12022)
                     /locus_tag="VIG70_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250518.1"
                     /translation="MISLERYAALLQQPDVRWTFVASMLGRLPIGITGLAILLLVQTS
                     SQSFARGGATTAFYVIGLAAVAPMLGRAIDRYGPRRILLTSGVLFPSALVALVAAVRD
                     GAPVLSLAFAAAAGASFPPISVCVRTYFRRRLSDGALLAAAYSAESVLIEIIFILGPM
                     LVAVFIAVASAAAAVWFAAACGLVGTLLFMRSPALLEWRIEQRTTGGLLGPLGERDFP
                     VLVGVVVCFSSAFGFMEIGVAAYASEIGNAAIAGVLLGVMSAGSALGGLVYGSRGWHS
                     PLGRQFALALALMAAGLAVLALGWRLWPFAFWCWLAGIAMAPGLIIQSMLAAKISRPE
                     HATEAFTWTTSALLAGVGIGLAAGGVLLERFPSQAALGAGAAAALLAAAGARFLLSR"
     gene            12128..13966
                     /gene="mnmG"
                     /locus_tag="VIG70_07935"
     CDS             12128..13966
                     /gene="mnmG"
                     /locus_tag="VIG70_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY8250519.1"
                     /translation="MLFPSEFDVIVVGGGHAGTEAALASARLGCRTLLLTHSIETLGQ
                     MSCNPSIGGIGKGHLVREIDALDGAMAIATDEAGIQFRTLNASKGAAVRATRAQADRT
                     LYRQAIRRRLENQPNLCLFQQAVDDILLEGDRAVGVATQLGLEFRSRSVVLTTGTFLS
                     GLIHVGLNSYQAGRAGDPASVTLAARLREMKLPVGRLKTGTPPRLDARSIDFNVLEEQ
                     PGDSPEPVFSFLGRREMHPRQLPCWITHTNLRTHEIIRAALDRSPMFTGVIQGVGPRY
                     CPSIEDKIHRFAGKASHQIFLEPEGLNTNEVYPNGISTSLPFDVQIELVHSIPGLERA
                     HLVRPGYAIEYDYFDPRELRSSLETKSVMGLFFAGQINGTTGYEEAAAQGLLAGLNAG
                     RRALEREPWCPKRNEAYLGVLVDDLVTRGVSEPYRMFTSRAEYRLMLREDNADLRLTE
                     AGRQLGVVSDARWNTFNRKRDAIAKETTRLKSTYVSAGYHERRSIYDILRRPEVHYPS
                     VAPSPFAEPEVAEQVEIQAKYEGYIQRQREEVARSAELESRALPPDLDYREVRGLSAE
                     VQQKLNRHRPETIGQAGRISGITPAAISLLLVHLKRGFRALDKKRA"
     gene            13963..14583
                     /gene="rsmG"
                     /locus_tag="VIG70_07940"
     CDS             13963..14583
                     /gene="rsmG"
                     /locus_tag="VIG70_07940"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY8250520.1"
                     /translation="MTPRAALDCGLGELGLELAPAAPERLLDYMALLVKWNRIYNLTA
                     IRDPLAMVTHHLLDSLSVLPHLPLSASRPRVADAGSGAGLPGIPLAIARPQWRVALAE
                     SSQKKAAFLRQAAIELALDNVEVHEGRVEAWRPQPLFDLVISRAFSALGEFIAACRHL
                     VVPGGTLAAMKGTRPRDLKAGCSLISLHVPLLDAQRHLVLCPASSQ"
     gene            14562..15335
                     /locus_tag="VIG70_07945"
     CDS             14562..15335
                     /locus_tag="VIG70_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY8250521.1"
                     /translation="MPRVLAVVNQKGGVGKTTTTVNLAAALAQAGKRVLLVDLDPQGN
                     ATMGSGVDKRTAARTVYHALLGLGELAGIRTRSEKGAYDLVPANRDLAGAEVELVDLP
                     ARETRLKSALDPISADYDYILIDCPPSLSLLTVNALAAAQRVLIPMQCEYYALEGLSD
                     LVGTVKRVRANLNPALEIAGLLRTMYDPRNTLSQQVSRELEAHFGDKVFRTLVPRNVR
                     LAEAPSYGVPAVVWDASSKGAQAYIALAAEVLEREVAYS"
     gene            15332..16165
                     /locus_tag="VIG70_07950"
     CDS             15332..16165
                     /locus_tag="VIG70_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY8250522.1"
                     /translation="MKAKGLGRGLDALLGGAEERPRDALLTLPIARIRPGRYQPRTKM
                     DQQALAELAASIRSQGLMQPVLVRPVDRDRYELVAGERRWRAAQMAGLEEVPALIREV
                     PDDAALAMSLIENIQRENLNPMEEAAGVQRLVDEFKMTHEQAADAVGRSRSATTNLLR
                     LLKLARPVQEMLMEGALEMGHARALLALDAARQIEAGKRVAAKGLSVRETEALVHTML
                     RGPAARRKRTDRDLARLEEETSERLGTTVEIRSGKKGTGKLVLHYSSLDHLDELLKKL
                     R"
     gene            16221..16577
                     /locus_tag="VIG70_07955"
     CDS             16221..16577
                     /locus_tag="VIG70_07955"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEY8250523.1"
                     /translation="MGKPILTVLKWQFAATVLATLAAALIAGVHAAASAAAGGAVSIV
                     AGLASALVAVLGKGNSAGGVLVGALRAEALKIGLALLLLWLVLSNYPQVAVGVLLGTF
                     VVTMLIFSMAFFVREY"
     gene            16590..17402
                     /gene="atpB"
                     /locus_tag="VIG70_07960"
     CDS             16590..17402
                     /gene="atpB"
                     /locus_tag="VIG70_07960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY8250524.1"
                     /translation="MAAGKALTPSEYIQHHLTFFTKPVGEGGGFWALNLDSIAVSILL
                     GLVGMGFIWWVVRGATSGVPGRRQAAVEWLIDFVDSQAKGIFQHGDRHRFVAPLALTV
                     FVWVVLMNAMDFLPADLVGLVTHRISDHGFRYVPTADVNTTFAMSISVLFLTLYFAIS
                     VKGVGGWVHELFCAPFGSHPLLWPANFLFQMVEYVSKTLSHSLRLFGNMYAGEILFLL
                     LWMWAATGVVGIIFGSFLGLLWAIFHILIVLLQAYIFMMLTVVYISMAHEHH"
     gene            17433..17705
                     /gene="atpE"
                     /locus_tag="VIG70_07965"
     CDS             17433..17705
                     /gene="atpE"
                     /locus_tag="VIG70_07965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY8250525.1"
                     /translation="MEKLQLLAMVQAYTGIGIGLMIGLGAAGACIGVGIMCSRFLEAA
                     ARQPELTNSLQGKVFLLLGLIDASFIIGLGIAMFFAFANPLATAFQ"
     gene            17729..18199
                     /locus_tag="VIG70_07970"
     CDS             17729..18199
                     /locus_tag="VIG70_07970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY8250526.1"
                     /translation="MNLNLTLIAQAITFTVFIWVTVKFIWPHMLRAIEARQKTIADGL
                     AAAEEGKRSLEQSNKRADEEIRKARERATEVLSQAEKRAAQMIDEAKNVAKEEGSREK
                     AAAKAEIAQELTRAREQLRDQVASLAVAGAEKILRREVDAKAHGDLLDSIKKQL"
     gene            18199..18735
                     /locus_tag="VIG70_07975"
     CDS             18199..18735
                     /locus_tag="VIG70_07975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY8250527.1"
                     /translation="MAEPTTVARPYAEAAFKLADAEGALGKWSDMLGVLAQVADDARV
                     RAAVADPNLSDAKAAGLFIGILAGNLSGDAENFVRVLAENGRLGLLPEVRAQFEALKN
                     EREGVLEAEVHSAFDLSEAQVADLVERLEKKTGRKVRAKIAIQKDLIAGVRIVLGDKV
                     IDGSARAQLGALETALKA"
     gene            18746..20293
                     /gene="atpA"
                     /locus_tag="VIG70_07980"
     CDS             18746..20293
                     /gene="atpA"
                     /locus_tag="VIG70_07980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY8250528.1"
                     /translation="MQLNPSEISELIKSRIQNLDAGARMRTQGTVVSVTDGICRIHGL
                     SDVMQGEMLEFPKGTYGLALNLERDSVGAVILGEYTHITEGDTVKTTGKILSVPVGPE
                     LLGRVVNSLGQPIDGKGAVNAKLTDVIEKVAPGVIARQSVSQPVQTGLKAIDAMVPIG
                     RGQRELIIGDRQTGKTAVAVDTIINQKGKDLFCVYVAIGQKASTIANVVRKLEEHGAM
                     AYTIVVAASASESAAMQYIAPYSGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNPAYVEKFTKGQVKGKTGSLTALPVIETQA
                     GDVTAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKIMKRRDTG
                     GGVRLALAQYRELAAFAQFASDLDEATRKQLERGRMVTELMKQAQYQPLQVWEMALTL
                     FAVNNGYFDDVDVKKALAAEKSMRDYVKSKFPIIVKSMEDKKDLSPEDEKSLHEAIKD
                     WKKNGSF"
     gene            20297..21160
                     /gene="atpG"
                     /locus_tag="VIG70_07985"
     CDS             20297..21160
                     /gene="atpG"
                     /locus_tag="VIG70_07985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY8250529.1"
                     /translation="MPGTKEIRVKIKSVQNTRKITKAMEMVAASKMRKAQERMRHARP
                     YGEKIRNVAAHISHANPEYRHPFLIDRDNVKRVGIIVITTDKGLCGALNTNLLRMVLA
                     QYKQWQAEGEEIEVCALGNKGFGFMQRLGAKIVSHAIQLGDRPQMEKLIGAVKVMLDG
                     YTSNRFDRLMLAYTRFINTMKQEPVIEQLLPLSGERLGAPETVWDYIYEPEARAVLDQ
                     VMTRYIEALIFQAVSENMASEQSARMVAMKAASDNAADLISELTLIYNKNRQAGITKE
                     LSEIVGGAAAV"
     gene            21186..22613
                     /gene="atpD"
                     /locus_tag="VIG70_07990"
     CDS             21186..22613
                     /gene="atpD"
                     /locus_tag="VIG70_07990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY8250530.1"
                     /translation="MAQAQGTIVQCIGAVTDIEFDRDSMPGIYDALILEDDPTNPLVE
                     KGLTFEVEQQLGDGVVRTIALGSSDGLRRGMKVKSSGGPISVPVGKGTLGRIMDVLGR
                     PIDEVGPIKSDKRRSIHQIAPKFDELSPSVELLETGIKVIDLICPFAKGGKIGLFGGA
                     GVGKTVNMLELINNIATQHSGLSVFAGVGERTREGNDFYHEMIEAGVVKLDNLEESKV
                     AMVFGQMNEPPGNRLRVGLSGLTMAEHFRDEGRDILFFVDNIYRFTLAGTEVSALLGR
                     MPSAVGYQPTLAEEMGRLQERITSTKTGSITSVQAIYVPADDLTDPSPATTFGHLDAT
                     VVLSRDIASLGIYPAVDPLDSTSRQVDPNTIGEDHYNTTRAVQATLQRYKELRDIIAI
                     LGMDELSPEDKLAVARARKIQRFLSQPFTVAQNFTGTPGKYVPLKDTIKGFKMIVAGE
                     CDALPEQAFYMVGPIEEAFEKAKKL"
     gene            22613..23035
                     /locus_tag="VIG70_07995"
     CDS             22613..23035
                     /locus_tag="VIG70_07995"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY8250531.1"
                     /translation="MAAPTIHVDVVSAEESIFSGEAEFVVLPGEAGELGIYPRHTPLI
                     TRIKAGAVRIKPAGGGEEQLIFVAGGILEVQPKVITVLADTAIRGADLDEAKANEALK
                     RAEEARAKAKDKQELATVEGELSTLAAQLAAIRKLRKR"
     gene            complement(23032..25395)
                     /locus_tag="VIG70_08000"
     CDS             complement(23032..25395)
                     /locus_tag="VIG70_08000"
                     /inference="COORDINATES: protein motif:HMM:NF018620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HEY8250532.1"
                     /translation="MQPTTRHALVVGAVCLASCAALTRDSLFDQRYGAPDPTRYDAPI
                     TQPGGAVSYRAEVRPILERRCVVCHGCYDAPCQLNLAAWEGVVRGANPEAVYQGSRLR
                     QAAPTRLFVDAKLASEWRKKGFFPVLNERTPTPEANLAASVLYRSLALKRAHPLPDVA
                     VLPKPFDFSIDRAQSCPRVEAFDAYEREFPLAGMPYGLPGLDERELGSLTRWLAAGAP
                     NDGPAPLTAELTRQVAAWEAFLNGSSLKERLMSRYLYEHLFLGFLYFETDTTRRAFRL
                     VRSATPPGEPVSLIATRRPYDDPGVPLAYYRLVPEQEAILSKTHLPYRLSPERMAKFR
                     AWFLAAPYEVSVFPSYDIESASNPFVAFAAIPVESRYRFLLDEAQYFIMNFIKGPVCR
                     GQLALDVIEDRFWVFFVDPQAGAGDASAELIARQADELRLPAEWGSNAPVVIPWLQYA
                     DLETRYLEAKNRALERSLAAPGALNLSLVWDGDNGRNRNAALTVFRHFDSASVVQGLV
                     GEPPKTAWVIGYALFERIYYLLVAGYDVYGNTGHQLNTRLYMDFLRMEGEFNFLVFLP
                     EAAREATRDHWYRSASRSVKDYVYGRKAQLNRETGIRYRTADPQRELYGLLRARLAPV
                     LAPRFDLSSIEDRALRRDLEALGTVRGASTVWLPEVAFLRVDDPPRAPRWFTLLRDTA
                     HSNVSNLFFEDKRLLPGENALTLVPGFIGAYPNAIWRVARAELPALAAAVGALASEDD
                     YRRLADRFAVRRTDPAFWVTSDAMADAYERSAPLEAGLFDYNRLENR"
     gene            25571..25759
                     /locus_tag="VIG70_08005"
     CDS             25571..25759
                     /locus_tag="VIG70_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250533.1"
                     /translation="MKHILDSDFRYRPSFDTDVRRTFEKVRRQQQVRDQTKTEPEIKE
                     VKVKVLKLEQLKKASGST"
     gene            complement(25761..26315)
                     /locus_tag="VIG70_08010"
     CDS             complement(25761..26315)
                     /locus_tag="VIG70_08010"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HEY8250534.1"
                     /translation="MKAIAVVLLVIGTWQFGRGAWIQAKAWVAQGLIAHAWSRTLQGA
                     ADVKPWGWADTWPVARLSVPRLGVERYVLSGADGASMAFGPGHVRGTSIPGGPGNSVI
                     GGHRDTHLAFMRKLQVGDALTVERADGARVSYRVAAADVLDRREVWVAKQEGPARLTL
                     VTCYPFDALSADGPLRYVVVALAE"
     gene            complement(26312..28348)
                     /locus_tag="VIG70_08015"
     CDS             complement(26312..28348)
                     /locus_tag="VIG70_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HEY8250535.1"
                     /translation="METEKHPRPTLPLLALLALASALGVAVAIALAGVAMLLAAPAYA
                     SQSETKEGMLYLRARGADPVAAPLLGTEVLFRISGPVARARVVQTFRNPHDVWYEGIY
                     VFPLPQSGAVDALRLVVGSRIVEGEIRERNVAKQAYAQARGAGQRAALLDQERPNIFT
                     TSVANIGPGETVVVELEYQQVVRYDSGRFSLRFPMVVGPRYIPAASLQVADAMRITPP
                     VMRPGTEGERTNPVAIQVMLDAGVPLAAIESAHHPIEVKAPSDSRREIVLAGGTTPAN
                     RDFELSWSPRPASLPQAAWFTEEKDGKHYGLLMLLPPAAPRSARLPREVIFVLDTSGS
                     MGGASIRQAKEALELALARLQPQDRFNVIEFNSFARALFDAARPADPDNLRAATRWVR
                     ALEARGGTEMAAALHLALDGADDPEHVRQVIFLTDGAVGNEEHLFGLIRARLGGSRLF
                     TIGIGSAPNSHFMTKAAQLGRGTFTYIGRVDEVRSRMGELFAKLETPVLKGVQAHWPS
                     GVAVEVWPARLPDLYAGEPVVLVARLDRLEGEVRLEGERNGTAWEARMPLAHNANAEG
                     LGAVWAREKVGALIDSLREGAPEAEVRARVIALAEAHRLVTKYTSFVAIDKTPARAPE
                     EALKLAEMPTLFPEGWDYAKVYGELPRGATDSRLALLLGTLAILLGLAVMRRFT"
     gene            28474..29163
                     /gene="pdsR"
                     /locus_tag="VIG70_08020"
     CDS             28474..29163
                     /gene="pdsR"
                     /locus_tag="VIG70_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HEY8250536.1"
                     /translation="MSRRIAIVEDEPAIRANYAEGLRKHGFEVAAYAGRGEALTAMRS
                     RLPDLALIDIGLGADVDGGFALCRELRALSAALPIIFLTARDSDFDTVAGLRLGADDY
                     LTKDVSFPHLLARIAALFRRAELAGDATAAPERLERGALTIDLKRFSVAWRGAAVPLT
                     LTEFWMVHALAKFPEHVKNREQLMREANLVVDDGTITSHIKRIRRKFLAVDPAFDAID
                     TVYGMGYRWQA"
     gene            29160..30677
                     /locus_tag="VIG70_08025"
     CDS             29160..30677
                     /locus_tag="VIG70_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8250537.1"
                     /translation="MKRPRFSLRVKLALVLLALAALPVAGILYVNEVERFLLQEQEQS
                     LLAVARAVATALHERPQLLASASGRDDEVDAILLGLQRASSRIWVVDGRYQVIAQAGS
                     LRRATEVASEAPWWQPLFVNTVGWVIPRPTEDFTEGPPEDLLATGSDVAAALQGTPAT
                     RARHTRDGRAVVLSAAHPIWTGDQVAGAVVTEETTNPVMSVKSAALERLLLLTLAAFA
                     GAAALLMAFATRLSMRIRRLRDEAESAIDAHGRIARLTASQADDEIGDLSRSFSAVLE
                     RLAEHHAYLEAMAGRLSHELRTPIAVVRSSLENLKISRENHDLYIDRAEQGLARLGRI
                     LERMTEASRLEQSLSAVERSRYDLAAVVRGCVEGYRIAYPKAAFALEVPERRVEVEGA
                     PDLAAQLLDKLVENAVDFSRGAAPVRVALEEAGGAAALTVSNKGPLLPDKMRTRLFES
                     MISVRDASGTATPHLGLGLYVARLIAQFHGGTIAASNLATGDGVALGVRIPLAWK"
     gene            30668..32125
                     /gene="glpD"
                     /locus_tag="VIG70_08030"
     CDS             30668..32125
                     /gene="glpD"
                     /locus_tag="VIG70_08030"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEY8250538.1"
                     /translation="MEMTYDLLVVGGGINGAGIARDAAGRGLSVTLVEKADLASATSS
                     SSSKLIHGGLRYLEHFEFRLVAEALAEREIMLRIAGHLTWPTRFVAPHVPELRPRWMI
                     RVGLFLYDHLARRSLLPASQAVRLDRPPYASGLRPELKHGFVYSDCRVDDARLVVVNA
                     LDAANRGARILVRTECVSARRVEGGWQAQLSNGETVLARAIVNAAGPWVKEVLNGPLG
                     ETTRDSVRLVKGSHIVLPRLYEGEHAFILQNDDRRVVFMIPFEDRFTLVGTTDIDYDG
                     DAAAVAASEAEVDYLCRAAGRYLARAPVPKDVLWRYAGVRPLYDDGSQDPSAITRDYT
                     LRVDDAQGAAPVLSVFGGKITTYRCLAESALDKLAPYFPGLKPAWTARAQLPGSDFAD
                     REATKRELQQRYSQVPPNVLQGVFRRHGTLAAQVLGDGRLGEYFGAGLTERELGYLVK
                     REWARTAEDVLWRRTKCGLRMTEAQRRRVAEVLSR"
     gene            32122..32898
                     /locus_tag="VIG70_08035"
     CDS             32122..32898
                     /locus_tag="VIG70_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEY8250539.1"
                     /translation="MKPLAELDRRGVRAVLLDIDDTLTTDGKLTAGGYGALERLKHAG
                     LRVVPVTGRPAGWCDHIARMWPVDAVVGENGAFYFFHEANRLQKRFQDSEAIRAGKRV
                     RLQAIAAQILAAVPGCALASDQPYRATDLAIDYCEDVPPLPVEAAQRIAALMRQAGLT
                     AKVSSIHVNGWFGEYDKLAATRLLFAERFGLDLAAVNREVVFAGDSPNDAPMFAFFEK
                     SVGVANVRRFEGLLAEEPKYVTRAASGAGFAELAEHLLRR"
     gene            33018..33455
                     /locus_tag="VIG70_08040"
     CDS             33018..33455
                     /locus_tag="VIG70_08040"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8250540.1"
                     /translation="MRALVFVAFLAASAAACAQSAEFAALDRNGDGFLSRIEAIAEPE
                     IAKRFAQFDTDKDRRLSPAEYRAAREDNERRAVRDAALAARVKEVLVATSGLPSKAIS
                     VEAYEGSVQLSGFVPVPDMASRAGRVVAAVNGVRSVHNNITVK"
     gene            33467..34690
                     /locus_tag="VIG70_08045"
     CDS             33467..34690
                     /locus_tag="VIG70_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY8250541.1"
                     /translation="MPGPLAGIRVLDLTRVLAGPWAAQNLADLGAEVIKVERPKKGDD
                     SRAFGPPWLKDAQGRDTAESAYFACANRGKKSLTLDLAQPQGQAIARELAARCDVLLE
                     NFKYGDLDRYGLGYAQLKKVNPGLIYCSVTGFGHTGPWRERPGYDFMIQGMGGLMSIT
                     GERDDRPGGGPQKAGIPIADLITGMYASIAVCAALAHRAKSGAGQHLDLALLDSLAAV
                     LANQGANYLATGESPGRLGNDHPNITPYQVFKTADGAVIVACGNDNLFRKFCEVAGCR
                     SLADDARFVTNGKRIANRVELTRLLSEILARRSTREWTAALEAAGVPCGPINNLEQVF
                     AEPQAVARGLKMELPHPTAGKVSLVRSPMRFSETPLQHEVPPPTLGQHTDEVLRDLLK
                     KNPSEIARLRSEGIV"
     gene            complement(34694..35665)
                     /locus_tag="VIG70_08050"
     CDS             complement(34694..35665)
                     /locus_tag="VIG70_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250542.1"
                     /translation="MKTLKWIASAALALTSIAGQAQQWPQKPMIFLNPFPAGGGTDTF
                     ARPIAAKLTQQLGESALIENQGGAGGTVGAANAAKRAPDGYNWFFGAVHHTIAESLYT
                     RLNYSLEKDFEPITVAAFVPNVVVVHPKHKDTLKTLKDLIDYAKANPDKLNYGSAGSG
                     TTHHLSVELFKTMTGTKLVHVPYKGAGPLMVDLLAGQVDLAFDGMGTSSTQVKAGKLI
                     PLAITSNKRNPVLPNVPTVQEAGVPGYEVRTWYGIWAIKGTPPAIKDRMYKEIVKAME
                     QPDLKRIWAEQGADAGGMPPAEMEKLVKSEIAKWAKVVKDAGAKVDN"
     gene            complement(35697..36893)
                     /locus_tag="VIG70_08055"
     CDS             complement(35697..36893)
                     /locus_tag="VIG70_08055"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8250543.1"
                     /translation="MSGPLAGVKVLELAQIMAGPTCGLMLADLGADVIKVERVPGGDD
                     TRRMDRPSVKGESASFMAMNRNKRGIALNLKLPTAQEALKRMAARSDVVTENYRKGTM
                     EKLGLGYEALRAVNPAIIYCSISGYGRTGPYAEKGGYDLIAQGMSGLMGVTGEAGRAP
                     VKSGGPVCDINAGLLGALGVVSAYVHRLKTGEGQLIDTSLFEAGIQQLYWQAAIHFAT
                     GEVPGPTGSAHILSAPYQAFRAADGWLNIGGANQANWERMCRVLGARDWLEDPRFRSN
                     ADRMKNLDALVPLMNARLKDRKVDDLIEALEAEGVPCGRINSIADVAADPQALAREMV
                     VELEHPRAGRTRALGLPIKLSRTPGKVSRPAPVLGQHTREVLEEFGFSRAEIDSLVGS
                     GAAVAA"
     gene            complement(36902..38101)
                     /locus_tag="VIG70_08060"
     CDS             complement(36902..38101)
                     /locus_tag="VIG70_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8250544.1"
                     /translation="MPRRAGRHFLQIPGPTNVPDRVLRAIDFPTMDHRGPEFAALGKE
                     VLAGMKRVFKSTLGEVVIYPASGTGAWEAALCNTLSAGDRVLMYETGHFATLWQKMAA
                     KLGLEVEFIASDWRRGADPEAIEKKLHADTDQPIKAVCVVHNETSTGVVSRIADVRKA
                     IDAARHPALFMVDTISSLASIDYRHDEWGVDVTVAGSQKGLMLPPGLSFNCVSPKAIS
                     ASKSARLPRSYWAWDEMIANGKSGYFPYTPATNLLYGLREALTMLLDEEGLDAVFARH
                     QRHAEAARRAVRAWGLEVFALDPREYSGSLTAVLMPAGHDADRLRKIILEAFDMSLGT
                     GLTKLGGKVFRIGHLGDFNDLTLMGTLAGVEMGLVLAGVPVKKGGVQAAMEYLASAAA
                     APARKVA"
     gene            38124..>40463
                     /locus_tag="VIG70_08065"
     CDS             38124..>40463
                     /locus_tag="VIG70_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8250545.1"
                     /translation="MNAPLTPIRFASVSHSSLAARLRKELDAEVMFDAASRGRYSTDA
                     SIYQVEPIGIVVPRSEEAARAAIAIAVDEGVPILPRGAGSSQCGQAVGAALVIDHTKY
                     LNKILEIKREQAVAVVQPGVVLDALNAELRKHGLWFPVDVSTSAQATLGGMAGNNSCG
                     SRSIAYGNMVHNVLAIEAVTASGQRWRFGPLSDARGEYGAFVQKLQQLYEREKAEIEA
                     RFPKVLRKVAGYNLDHLGPPHANAAHLLVGSEGTLAYSERLHLKLSPLPAKRSLGVCH
                     FPKFYTAMELTQHIVKLGPTAVELVDRTMIGLAREIPAFEKTVNAFIRGDPDAILLVE
                     FADDRVGRLKDLVQLMADLGLPGSVVEIPDEKLQKQLWDVRKAGLNIMMSMKGDGKPV
                     SFIEDCAVPLEHLAEYTERLTRVFEKHGTRGTWYAHASVGTLHVRPVLDMRRDGAEKM
                     RAIAEEACAMVKEYKGAYSGEHGDGLVRSEWIAPFFGPRLTAALAEIKSWLDPRGLMN
                     PGKIVHPSRMDDKSLFRFKPGYATSVPATVLDWGEWGGYDKAVEMCNNNGHCRKFDAG
                     TMCPSYRATMDERHLTRGRANTLRLALSNQLGDQGDEAVKEALDLCVSCKGCKRECPT
                     GVDMARMKIEFLARYKATHGLSARDRAIGYLPRYAPWAARLAPVSNAVSSLLKSSLGF
                     AAKRELPKWRSDVFTEQGRNDGDVVLFVDTFNRYFEPENARAALKVLEAAGHRVHAPR
                     GLCCGRTFLSVGLVDEAKREARKMLDALSPYAAKGIPIVG"
BASE COUNT         6863 a        13459 c        13514 g         6627 t
ORIGIN      
        1 tcgatgaacg cgcggcgcgg ctcgacctcg tcgcccatca gcttggtgaa gatctcgtcg
       61 gcggcgatgc cgtcctcgat ctgcaccctg agcaggcgcc ggctggccgg atccatcgtc
      121 gtctcccaga gctgcgccgg gttcatctcg cccaggccct tgtagcgctg cagggtcatc
      181 gagcgctcga cctcgcccag cagccacttc atcgcttcgg cgaagtcgcg caccggctgg
      241 cgcttgtcgc cgcgctgcac gctggcgccg gcgcccacca gcccgcgcag catctcggcg
      301 gtgctgcgga tctgagcata gtcgccggat agaacgaact cggtgtcgat cgtgctcagg
      361 cgcacgttgc cgtggcgcgt gcgctcgacg cgcacctggt ggcgctcggt cttctggtcg
      421 aagtgcggcg ccacggcgaa tccctgcggg gcgagcgccg cctccagcgc ttcggccgaa
      481 gcttccgccg tatcgcgccg cgacaggtcg acctgcacac cgcgcagcat cgcctgcagc
      541 acctcgcggt cgaccaggcg cgagacgcgc tcgatcaccg cttccgacaa cagataggac
      601 ttggcgagct cggcgagcgc ctcgccgctg atcggctcgc gcccgtcgcc cggatgcaga
      661 gcggcgtcga ccagcgcctg cttgagcatg aacgcgttca gctcgtgctc gtccttgagg
      721 tagcgctctt ccttgccaag cttggccttg tagagcggcg gctgcgcgat gtagatgtgc
      781 ccgcgctcca ccagctcgcg catctggcgg tagaagaagg tgagcagcag ggtgcggatg
      841 tgcgagccgt cgacgtcggc gtcggtcatc acgatgatgc ggtgatagcg cagcttgtcg
      901 gggttgtatt cctcggcgcc gatgccgcag ccgagcgcgg tgatgagcgt cgcgatctcg
      961 gccgacgcca ggagcttctc gaagcgcgct cgctccacgt tcagtatctt gcccttgagc
     1021 ggcaggatcg cctggaactt tcggtcgcgt ccctgcttgg ccgagccgcc ggccgagtcg
     1081 ccctccacca ggtagagctc ggacttcgcc gggtcgcgct cctggcagtc ggcgagcttg
     1141 cccggcagcc ccatcgagtc gagcacgcct ttgcggcgcg tcatctcgcg cgccttgcgc
     1201 gcggcttcgc gcgccgccgc cgcctcgacg atcttcaccg tgatggtgcg cgcgtcgtgc
     1261 ggacgctcgt cgaggaactc gcgcaggcgc tcggcgacga tctcctcgac caccgggcgc
     1321 acctccgagg acacgagctt ctccttggtc tgcgacgaga acttgggctc gggcaccttg
     1381 accgacagca cgcaggcgag cccttcgcgc atgtcgtcgc cggtggtctc gaccttggcc
     1441 tttttcgcga tctcgttttc ctcgatgtac ttgttgagca cccgtgtcat ggcggcgcgc
     1501 aggcccgtca ggtggctgcc gccgtccttc tgcgggatgt tgttggtgaa gcacaggacg
     1561 ttttcctggt acgaatcgtt ccactgcatc gccacttcga ccgtaaggcc gtccttctca
     1621 ccgtggccga gaaagacgtt ggggtggagc acattcttcg cgcggttcat gtactcgacg
     1681 aagcccttca ccccgccgga gaacgcgaag ttctcctcct tgccggcgcg ctggtcgatg
     1741 agcacgatct tgacgccatt gttcagaaac gacagctctc gcaggcgccg cgccaggatg
     1801 tcgtagtggt agtcgatggt gccgaaggtc tccttcgagg cgaggaaatg cacctcggtc
     1861 ccgcgccgct cggtgctgcc ggtcgccttg agcggcgcca cggccacccc gttgcggaat
     1921 tccatctggt gcacctgccg ttcgcgccag acggtcagcc gcagccactc cgagagggcg
     1981 ttcacgaccg agacaccgac gccgtgcagc ccgcccgaca ctttgtagct gttggagtcg
     2041 aacttgccgc cggcgtgcag ctcggtcatc acgatctcgg cggccgagcg ggcgagggtg
     2101 tcgtccagct tgacgccggt ggggatgccg cggccgttgt cgatcaccga caccgagttg
     2161 tcggtgtgga tggtgacgat gatctcgtcg cagaacccgg cgagcgcctc gtcgaccgag
     2221 ttgtcgacca cctcgaacac catgtggtgc agcccggtgc cgtcggaggt gtcgccgatg
     2281 tacatcccgg ggcgcttgcg caccgcctcc aggcccttca ggaccttgat ggcatccgag
     2341 ccgtagtcgc cgctctcgcc gttgcctttg tgctccgaca tgctcttcct agatcctcat
     2401 cggcatgacg acgtacttga agtccttttc cgccccgtag gtgatgagcg cgctcgacgc
     2461 ggaatcgccg aaggcgcact cgatgacgtc gcccgacacg ttgttcagca cgtcgagcag
     2521 gtagttgacg ttgaaaccga tgtcgagcgc gtcgccggaa tacttcacct cgagctcctc
     2581 gtgcgcttcc tcctgctcgg cgttcgaaga cacgatcttc aggctgccgt cggcgagcac
     2641 ccagcgcacg ccgcggaatt tctcgttcga caggatggcc gctcgcagca acgcctgacg
     2701 cagcggctcg cgctcgatct gcagacggtt cttgtggccg gtcggaatga cgcgtgtgta
     2761 gtccggaaac ttgccgtcga tcagcttcga caccaggtcg atgtcgccga agctgaacga
     2821 agcctgggtg gccgcgatct cgatcttcac cggcgcatcg ctgtcggcaa gcagcttgcc
     2881 gagctcgagc acggttttgc gcggtacgat cacctcctgg cgcggcagct tcgcgcccag
     2941 cttcagcgcc gcgaatgcca ggcggtgacc gtcggtcgcg accaatttca ggctgccttc
     3001 ctcgacgacc atcagcaggc cattcaggta gtaacggatg tcctgctgcg ccatggcgta
     3061 ttgcacgaga gacagcaggc ggcgcaacgc cttctgctcg agctcgaacc tggccgactc
     3121 gcccgccggc ctggcgaggc gcggaaagtc ctcggccgcg agcgtctgca gcgtgaaccg
     3181 gctcttgccg gccttgacca gcagccgctt gtcctgctgc tgcagcgtga cctccgcgct
     3241 ctcgggaagg gcgcgcagga tatcgaggag cttccgggcg cccacggtga ccgcgcgcac
     3301 gtcggccggg gcgccgatgc cgctgcgcgc ggcgatctgg atctcgatgt cggtggcgag
     3361 gaacgcaagc atgtcctgcg cgcgctcgat cagcacgttc gacaggatcg gcagcgtatg
     3421 gcggcgctcg atgatcccgc tcacggcggc aagcgggccg aggagctcgt cgcgctttgc
     3481 tttagcaaga ttcattgtta tttaccttag tagtaactaa tagagcgccc tgatatgcgg
     3541 gataagcctg caactccttg ccgcagcgcg cattgcaccg tattcggaca ggcgcaaagc
     3601 ctgtggagat cgcaagatga tttgtgaata gttcctgccg caacgccgat cgctgtcctt
     3661 attcacaaac tgtccccagg ctgtccccag ccctcagccc ttgagcacct gctcgaggac
     3721 cacatagtct cgattgagcc cggtgtcgtt cttgcgcagg ctggcgatcg tgcggcaggc
     3781 gtgcagcacc gtggtgtggt cgcggttgcc gaacgcctcg ccgatctcgg gcaagctcat
     3841 ctgcgtcagg tccttggcca gggccatcgc gacctggcgg ggccgggcca cgttgcggct
     3901 gcgcttcttg gaatgcatgt ccgagatctt gatcttgaag tactcggcga ccgtcttctg
     3961 gatgccctcc acgctgacct ggcggctggc cacgttcagg atgtcctgca gcgcttcctt
     4021 ggcgagctcg agcgacagct cgcgtccgga gaagcgcgca aaggcgagca ctttcttcag
     4081 cgcgccttca agctcgcgca cgttggagcg caggtgcttg gcaatgaaga acgcgatgtc
     4141 ctccgacaga gcgaccgact ccgcctcggc cttcttgagc acgatcgcga cccgcatctc
     4201 gagctcggga ggctcgatcg ccaccgtcag gccccagccg aagcgcgaga tcagccgctc
     4261 ctccatcccg gcgatctcct tgggataggt gtcgcaggtg atcacgatct gcttatgcgc
     4321 ttcgaccagc gcattgaaag cgtagaagaa ctcctcctgg gtgcggccct tgttggagaa
     4381 gaactggata tcgtcgatca gcaagagatc caacgagtgg tagtagcgct tgaactcgtc
     4441 gaacgacttc tgctggtaag cgcgcactac gtcggtgaca tacgcttcgg cgtggatata
     4501 ccggatgcgc gcctgcggcc gctgatcggc gagctggttg ccgatcgcct gcaccaggtg
     4561 ggtcttgccc agtcccaccc cgccgtagac gaaaagcggg ttgtaggaga cgccgggatt
     4621 ctcggacacc tgcatcgcag ccgcgcgcgc aagctgattc gctttccctg tcacgaagct
     4681 gtcgaaggag aacgcgggat tcagccgcgc gcgctcgaag atcgacggag tttccgcggg
     4741 ccgccgatgt gcatccccgg gggctggcgc caagcgcgcc ggcgcttcgg ccacgcgggc
     4801 tagcaccagg cgcagcccga gctcgcgccc gctcgcttcc gcggcgaggc gctcgatgcg
     4861 cgccgcgaag cgctcgcgca ccagctcgag gacaaaccgg ttcggggccg tgaggaccaa
     4921 ggtgtgagct gcagtgtcgg ttgcctcgcc ggattccggc acgagcggcc ggatccacgt
     4981 gttgaactgc tgcgcgggga gctcctgctc gagccggcgc aagcaggcat cccagaggtt
     5041 ttgcatcttt tcgttgggcc tgaagcgagt cgagatttta ccttcttggc cctgccttat
     5101 ccacagacga attgacaatg cgctgacgcg cacgctctaa taccgccctt tttccggatc
     5161 ggcagagatg gccacgaagc ggacctacca accttccaaa acgcggcgcg cgcggcgcca
     5221 cggttttctc gtgcgcagcc gtactccggg cggacgcaag gtgctgcgta atcggcgcgc
     5281 ccgcggtcgg cgccgcctcg ggctgtagcc gcggccggca agcgcttcgg gctggatgcg
     5341 gcgcgccggc tgaggcagaa agccgaattc gaaaccctgc tgcgacacgg cgcgcggcgc
     5401 agcctggacg gctacacctt ctatgtggcg cggcgcgaaa ccggaagtgc gcggctcggc
     5461 atgctgattt cccgcaagca cgccgcgcgc gccgtcacgc gcaacgggct gaagcgctgc
     5521 attcgcgaag ccttccggct ggagcgggag cggctcggtt cgctcgacgt gctggtgcgg
     5581 ccgccttatg gcgcaaagcc ttcggccgag atgattctgc ggctgcgcga tatcttcggc
     5641 aggctcaact catgatcgtg cgcgcgttgc gcttcgcgct tcaaggctac cgctggtgcg
     5701 tgcgtccgtt gctgccgccg gcctgccgct tctacccgag ctgctcgcac tacgccgagg
     5761 aagcgctggt gcgccacggc gcctggcgcg gcggctggct tgcggcgtgg cgcgtgtgcc
     5821 gctgcggccc ctggtcgccc ggcggccacg atcccgtacc ctgaacacga tggacaccca
     5881 aagactcatc ctcctggtga ttttcagctt ctcgctgctc atgctgtggg aagcctggga
     5941 gaaggagaag cggccgaagc ccgcacccca ggcgcccgcc gcgcagcagg gcgtgccggt
     6001 gccgggcaag cccgcgccgc ctgccgccca agcgccgggt gtggctccca agccggctcc
     6061 cgctgcggtc ccggcagcgg ccgcggcggc gaagggcgag atcgtgcgcg tgagaaccga
     6121 tttgctggtc gccgagatcg ataccttggg cggcacgctg aaacgcctgg agctgctgaa
     6181 gcataaggac tcggccgatc cgaccaagaa cttcgtgttg ttcggccctg accatcaata
     6241 cgaggcacag agcggcatca cgcgcgaagg cgggccgaac cacctcacgc tatggacggc
     6301 gggcgcgaag gacgtggctc tcgaaccagg aaaggatgct gtcgaggtcc gcctgaccgg
     6361 tgccagccgc gatggcgtca gcgtcgagaa ggtctacctc ttcaagcgcg acagttatgt
     6421 cgtcgatctg tccctggaag tccagaaccg cagctcggag agcctgaccc cctacgccta
     6481 tttccagttg actcacgacg gcaagccgac cagcaacgtc aacaccgtgg cggagacctt
     6541 cggtgcccag agctttaccg gctttgccct ctacaccccc gagaaggcgt tcgagaaggt
     6601 ccattccagc gatctcgaca aggccaagtt cgagcggcgc gccgacaacg gctggctcgg
     6661 cttcgtccag cactacttcg tatcagcctg gattccgccg gacaaggtgg cgcgcgacta
     6721 tggcgccctg aagcgcaacg acggcctgta ctcgggcaac gtggtcctgc agccgggcac
     6781 ggtggccccg ggagcttccg cgcaggtcaa ggtgtcgctg tacgcggggc cgcaggagca
     6841 gcgccggttg caggctgccg cgcccgggct cgacctggtg gtcgactacg gctggctcgc
     6901 gatcatcgcc tggccgctgt tctggctgct ggagaagttc catgcgctct cgggcaactg
     6961 gggcgtcgcc atcatcctgc tgacggtgct gatcaagatc gtgtttttcc cgctctcggc
     7021 ggcgagctac aagtccatgg ccaaaatgaa gttgatcacg ccgcggttga ccaagctgcg
     7081 cgagatgtat gggaacgacc gccagaagat gaaccaggcc atgatggagc tctacaagac
     7141 cgaaaagatc aacccgctcg gcggctgctt cccgatcctc gtgcagatcc cggtattcat
     7201 cgcgctctac tgggtgctgc tcgcggcgat cgagctgcgc cacgcgccgt tcatgctgtg
     7261 gatcaaggat ctgtccgcgc tcgatccgta ctacgtgctg ccgatcctga tgacgatgac
     7321 gatgatcctg caaaccaaga tgaacccggt gccgcccgac ccggtgcagg cgagggtgat
     7381 gcagatcatg ccgttcgtct tcagcatctt tttcttcttc ttcccggccg ggctggtgct
     7441 ctattggctg gtcaacaaca tcctttccat cgcccagcaa tggcagatcc agcgcatgtt
     7501 cgatcgcgac aggcctgccc atgccaagcg atgaggtgat tcgggacacg atcgcagcga
     7561 tcgccacgcc caccggacgc ggcggaatcg gcgtggtgcg catctcgggt cccgacgtcg
     7621 ccggaatttg ccaaaagctg ctggggaagc tgccgccgcc ccggcatgcc acgcacgccg
     7681 tatttcgcga cgcccagggc gaggcgatcg acgaaggcat agcgctgtat tttccggcgc
     7741 cgcattccta cactggagag ccggtgctcg agctgcaggg gcacggcggt ccgatggtga
     7801 tgcaggcggt gctcgcagcc tgcctggatg cgggggcccg gcttgcggag cctggcgagt
     7861 tcactcgaag ggcgtttctc gaggggaagc tcgatcttgc gcaggccgag gcggtcgccg
     7921 acctgattga cgcggcgagc cgagaagcgg cgcgctcggc gctgcgctcc ctcaccggag
     7981 agttctcggc cgcgatcggc gccatgcagt cgcagctgat cgaattgcgc gcactcaccg
     8041 aagcaatgct cgatttcccg gaagaggacg tcgacgcctt ccaccggggg gatgctgccg
     8101 ggcgcctggc cgcggtgcga ggcgcgctcg acgatgtact cgccaagagc cgtcagggga
     8161 gcttgctgcg cagcggtatc cacgtggtgc tcgcaggacg gccgaacgtc ggcaagtcga
     8221 gcctcctcaa ccggctcgcc ggcgaggaac gcgcgatcgt gacgccggtg gccggcacga
     8281 cgcgcgatgc gctgcgcgag ccaatccaga tcgagggcgt ggcgctggtg ctggtggaca
     8341 cggccgggct gcgcctgtcc tccgacatgg tcgagcgcct cggcgtcgag cgcacgcagc
     8401 gcgagctcga gcgtgccgac gtcgtgatgg ccgttttcga ggccggcaaa ggccgcgatg
     8461 cgctggagaa cctgccctcc ggcgtgccgc taatcgaggt ctacaacaag atcgacctcg
     8521 ttcccggctt cgccgcgcct gccggggcgg tggcagtgtc ggcgaggacc ggcgaaggcc
     8581 tcgccgagct gcgtaaagcc atacttgagg ccgccggctg gtcgtcgagc ggcgagccgg
     8641 tgttccttgc ccgggagcgt cacctgcggg cgctggagag agcgcgtgct cacctcgagg
     8701 cggcgactcg cgaaaccggg cgctgggagc tttttgccga agaactgcgc ctagcgcaca
     8761 ccgcgctcgg gagcgtcgtg ggcgaattca gcgccgacga tctgctcggc gaaatctttg
     8821 cgcgcttttg catcggcaag tagctcgact accataagta gttgtttatt tacagcatta
     8881 tcgtcattag gctaaacggg ccctagaggc cgaaacatcc cccgatgcta gaatcgcagc
     8941 cgcacggtac tctcggtgcg accggcccag cctgcgctgg gcctttgcct ttctggagtg
     9001 agccagcgaa atgaatgctc ccggtagcac cctcatcgac cagtcgaacg caccgtcctg
     9061 ggtgcgccat cgcggcctgc ggcaatgggt tacggacgtc gcccggctgg cgaagcccga
     9121 acgcatcgtc tggtgcgacg gttcgcaagc cgaatacgac cggctgtgcg ccgagctcgt
     9181 cgcgagcgga accttcattc ggttgagcga aaagctgcgg cccaactgct acctcgcccg
     9241 ctcgcatccg agcgacgttg cccgcatgga ggatcgcacc ttcatctgca gcgaggagaa
     9301 ggaggacgcg ggcgcgacca acaactgggt cgatccgcgc gagatgcgcg ccaccctgaa
     9361 ccgcctgttc gacggttgca tgcgcgggcg cacgatgtac gtaatgccgt tctccatggg
     9421 gccgatcggc tcgccgatcg cgcaagtcgg cgtcgagatc tccgattccg cgtacgtcgc
     9481 ggtgaacatg cgcctcatga cgcgcatggg acgcgcggtc gccgactggc tcggcgagga
     9541 cggcgccttc gtcccttgcg tgcactcggt cggcgctccg ctgacggcgg gaaaaaagga
     9601 cgtcgcgtgg ccgtgcaacg agaaggaaaa atggatcgtg catttcccgg agacgcgtga
     9661 gatttggtct tacggctccg gctacggcgg caacgcgctc ctcggcaaga agtgccttgc
     9721 gctgcgcatc gcgtcggtca tggcgcgcga cgaaggctgg ctcgccgagc acatgctgat
     9781 cctcggcgtg caggcgccca cgggtgaaaa gacctacgtc gctgcggcgt ttccctcggc
     9841 atgcggcaag accaacctcg ccatgctgat cccgccggcc gccttcgccg actggaaggt
     9901 gacgacgatc ggcgaggata tcgcctggat caagccgggt gccgacgggc gcctgcgggc
     9961 gatcaacccc gaatacggcc tgttcggcgt tgcgcccggc acttcagagc agaccaatcc
    10021 gaacgcgatg aagatgctgc actcgaacgt gatcttcacc aacgtcgcgg tcacgccgga
    10081 cggcgatgtg tggtgggagg gcatgaccga caagccgccg gccgggctga ctgactggca
    10141 aggaaagccc tggaccccgg aggacggcaa gaagggccgc ctcgcggcgc atgccaacgc
    10201 ccgcttcacg gtgcctgcct cgcagtgccc gtccatggac gccgcctggg aggatcccgc
    10261 cggggtgccg atttccgcgt tcgtcttcgg cggccggcgc tcgaccaccg tgccgctggt
    10321 tgtcgaggca ccgagctggg aagagggcat ttacatggcg gctacgcttg gctccgagac
    10381 caccgcggct attaccggga agcttggtgt ggtgcgccgc gacccgatgg cgatgctcgc
    10441 cttctgcggc tacaacatgg gcgactactt cgcccactgg ctgaagatgg gccgctcgct
    10501 gaagcagccg cccaagattt tccgcgtcaa ctggttcagg cgcgatgcga acggcaagtt
    10561 cctctggccg gggttcagcg agaacatgcg tgtgctcgcc tggatcgtcg cgcgctgcca
    10621 gggccgggca cagggcgccg acacggcgct cggcctcatg ccgcgctacc aggacctcaa
    10681 ctgggccggc ctggaaaaga tgaccgaagc gcggttcgcc gagctgtcgc aagtggatgc
    10741 ggcagcctgg aaggaagagc tgcgctcgca cgacgagctc ttcgccaagc tcggcgcgcg
    10801 gctgccgccc gcgctcgagg cgcgccgcgg ccgcatgcac gagaagctag cggcttaaca
    10861 ggaaacgggc gccggcggcg gcgagcagag cagccgcggc gcctgcgcca agcgcggcct
    10921 gggagggaaa gcgttcaagc agaacgccgc cggcggccaa tccgattccc actcccgcga
    10981 gcagtgcgct tgtagtccag gtaaacgctt cggtggcgtg ctcgggacgc gagatcttcg
    11041 cggcgagcat cgactggatg atcagccccg gcgccatggc gatgccggca agccagcacc
    11101 agaacgcgaa cggccacagc cgccagccta acgccagcac cgccaagccc gccgccatca
    11161 gcgcgagcgc gagcgcaaac tggcgtccca agggcgagtg ccagcctcgg ctcccgtaga
    11221 ccagcccgcc gagggcactg cccgcgctca tgactcccag aagcacacct gcaatcgcag
    11281 cgttgccgat ttcgcttgcg taggcggcca cgccgatctc catgaagcca aaagcgcttg
    11341 aaaagcacac cacgacgccg accagtactg gaaaatcgcg ctcgccaagg ggacccagaa
    11401 ggccgccagt cgtccgctgc tcaatccgcc actcgagcaa agccggagag cgcatgaaaa
    11461 gcagcgtgcc cacaagcccg cacgcagccg cgaaccatac ggccgctgcc gccgacgcca
    11521 ctgcgatgaa caccgcgacc agcatcggtc ccaggatgaa gatgatttcg atcaataccg
    11581 actccgcgga gtaggccgcg gccagcagcg cgccatcgct aaggcgccgc ctgaagtagg
    11641 tgcgcacgca gacggagatg ggaggaaagc tggctcccgc cgcggcagcg aatgccaggc
    11701 tgaggaccgg cgctccatcg cgtacggcag ccaccagagc caccaacgcc gaagggaaga
    11761 gcacgccgct ggtgagaagg atgcggcggg ggccgtagcg gtcgatcgcg cggccgagca
    11821 tcggcgctac tgccgccaaa ccgatcacgt aaaaagcggt ggttgctccc ccgcgagcga
    11881 aagactggct gctggtctgt acgagcagga gaatggcgag gccggtgatt ccgatcggca
    11941 gccggccgag catcgaagcg acgaacgtcc agcgaacatc cggctgctgc agaagcgccg
    12001 catagcgctc tagagaaatc acttaagaaa tcacttaatg ctgcgctgca gcaaagacgt
    12061 tgttccacgt ggaacgagag cgctcccgtg gttccacgtg gaacgatcac gtaaaattcg
    12121 ccaccccatg ttgttcccta gtgagttcga cgtcatcgtt gtcggcggcg gccacgccgg
    12181 taccgaagcc gccttggcct ctgcgcgcct cggctgccgc acgctcctcc tgacccacag
    12241 catcgagacg ctcgggcaga tgtcgtgcaa cccgtcgatc ggcggaatcg gcaagggcca
    12301 tcttgtccgg gaaatcgacg ccttggatgg cgccatggca atcgctaccg acgaggccgg
    12361 gatccagttt cgcaccctca acgcctcgaa aggcgcagcg gtgcgcgcca cgcgcgccca
    12421 agccgaccgt acactctacc gtcaagccat ccgccgccga ttggaaaacc agccgaacct
    12481 ctgcctgttt cagcaggcgg tcgacgatat tctgctcgag ggggatcgcg cggtcggggt
    12541 ggcgacccag ctcggcctcg agttccgcag ccgctcggtc gtgctgacca ccggcacctt
    12601 cctctcaggc ctgatccacg tcggactgaa cagctatcag gcagggcgcg ctggcgatcc
    12661 tgcctcggtc acgcttgcag cgaggctccg ggagatgaag cttccagtcg gccgcctcaa
    12721 aacaggcacg ccgccgcggc tagacgcccg cagcatcgac ttcaacgtgc ttgaggagca
    12781 gccaggagac tcgcccgagc cggtgttttc cttccttggc cggcgcgaga tgcatccgcg
    12841 gcaacttccg tgctggatca ctcacaccaa tctccgcacg cacgaaatca tccgcgcggc
    12901 gctggaccgc tcgccgatgt ttaccggcgt catccaaggc gtgggcccgc ggtactgccc
    12961 ctcgatcgag gacaagatcc atcgctttgc cgggaaggcc agccatcaga tcttcctgga
    13021 gcctgaaggc ctgaacacga acgaggtgta cccaaacggc atttccacca gcctgccatt
    13081 cgacgtccag atcgagctgg tgcactcgat ccccgggctc gagcgcgcgc acctcgtacg
    13141 accgggttat gcgatcgagt atgactactt cgacccgcgc gaacttcgca gctcgctcga
    13201 gacgaagagc gtgatgggct tgttcttcgc cgggcagatc aatggcacta caggctatga
    13261 agaggcggcg gcacaggggc tgctggccgg cctgaacgcc gggcggcgcg ccctggaacg
    13321 cgagccctgg tgccccaagc ggaacgaggc ttacctcggg gtgcttgtgg acgacctcgt
    13381 aacgcgcggc gtatccgagc cataccgtat gttcacgagc cgtgccgagt accggctaat
    13441 gctgcgcgag gacaacgcgg atcttcggct gacagaggcg gggcgccagc tcggcgtggt
    13501 gtccgatgca cgctggaata cgttcaaccg caaacgcgac gccatcgcca aggaaacaac
    13561 gcgattgaaa tctacgtatg tgagcgctgg ctatcacgaa agacgctcta tttacgacat
    13621 tctacgccga ccggaagtgc attatcccag cgtcgcgccg tcaccgtttg cggagccgga
    13681 agtcgccgaa caggtagaaa ttcaggccaa gtacgagggg tacatccaac gccagcgcga
    13741 ggaggtagcc cgcagcgccg agctcgagtc gcgcgcgttg ccgccggacc tcgactatcg
    13801 cgaggtacga ggcctgtcgg ccgaggtgca gcagaagctg aatcgtcacc ggccggaaac
    13861 catcggacag gcagggcgca tctccgggat cacccccgcc gcgatctcgc ttttgctggt
    13921 gcacctgaaa cggggcttcc gggcgctcga caaaaagagg gcatgactcc gcgagccgcg
    13981 ctcgactgcg ggctcggcga gttagggctg gagctggccc cggctgcgcc cgaacggctg
    14041 ctcgactaca tggcgctgct tgtgaaatgg aaccgcatct acaacctgac cgcgatccgc
    14101 gacccgctcg ccatggtgac gcatcacttg ctcgattcgc tttcggtgct gccgcacctg
    14161 ccgctctcgg cctcacggcc tcgagtcgcc gacgcggggt cgggcgcggg gctaccgggt
    14221 attcctctcg cgatcgcgcg cccgcaatgg cgcgttgcgc tcgccgaatc gagccagaag
    14281 aaggcggcgt tcctgcgaca ggcggcgatc gagctcgccc tcgacaatgt cgaggtgcac
    14341 gagggacgcg tcgaggcatg gcgcccgcaa cccttgttcg atctggtgat ctcgcgcgcc
    14401 ttttccgcgc tcggcgagtt cattgccgcg tgccggcatc ttgtcgtccc cggcggaacg
    14461 ctcgcggcga tgaaggggac aagaccaagg gacctgaagg ccggttgttc gctgatttcc
    14521 ctgcacgtgc cgcttctcga tgcgcaacgc catctggtgc tatgccccgc gtcctcgcag
    14581 tagtgaatca gaaaggcggc gtgggcaaga ccaccactac cgtgaacctc gccgcggcgc
    14641 tggcgcaggc ggggaagcgc gtgctcctcg tcgatctcga tccgcaggga aacgcgacca
    14701 tgggcagcgg tgtcgacaag cgcacggcgg cgcgcaccgt gtaccacgcg cttctcgggc
    14761 tgggcgagtt ggcgggaatc cgcacgcgtt cggagaaggg cgcctacgac ctggtgcccg
    14821 caaaccgcga cctcgccggc gccgaggtcg agctggtcga tctgcccgcg cgcgaaacgc
    14881 ggctcaagtc tgcgctcgac ccgatcagcg ccgactacga ctacatcctc atcgactgcc
    14941 cgccgtcgct cagcctgctc accgtcaatg ccctcgccgc cgcgcagcgc gtgctgatcc
    15001 cgatgcagtg cgagtactac gcgctggaag gactctccga cctcgtggga accgtaaaac
    15061 gggtgcgcgc caacctgaac cccgctctcg agatcgcagg gctcctgcgc accatgtacg
    15121 acccgaggaa caccctttcg cagcaggttt cgcgcgagct cgaagcgcat ttcggcgaca
    15181 aggtgttccg cacgctcgtg ccgcgcaacg tgcggctcgc cgaagcgccg agctatggcg
    15241 ttccggcggt ggtgtgggat gcgtcgtcca agggagcgca ggcgtacatc gcgctcgcag
    15301 ccgaagtgct ggaacgcgag gtggcctact catgaaagca aaaggtcttg gacgggggct
    15361 cgacgccctt ctcggtggcg cggaagaacg gccgcgcgac gcgctgctga ccttgccgat
    15421 cgcgcgcatc cggccgggac gctaccagcc gcgcaccaag atggaccagc aggcgctggc
    15481 cgagcttgcc gcctcgatcc gctcgcaggg gctgatgcag ccggtgctgg tgcgtccggt
    15541 cgatcgcgac cgctacgagc tcgtcgccgg cgagcggcgc tggcgcgcgg cgcagatggc
    15601 ggggctggag gaggtgccgg cgctgatccg cgaggtgccc gacgacgccg cgctcgccat
    15661 gtcgctcatc gagaacatcc agcgcgagaa cctgaatccc atggaagagg ccgcgggcgt
    15721 gcagcggctg gtcgacgagt tcaagatgac ccacgagcag gccgccgacg ccgtcggccg
    15781 ctcgcgtagc gccaccacca acctgctgcg cctgctcaag ctcgcgcggc cggtgcagga
    15841 aatgctgatg gaaggcgcgc tcgagatggg gcatgcgcgc gcgctgctcg cgctcgacgc
    15901 cgcgcgccag atcgaggcgg gcaagcgggt cgcggcgaag ggactttcgg tgcgcgaaac
    15961 cgaggcgctg gtgcacacga tgctgcgcgg tcccgcggcg cgccgcaagc gcaccgaccg
    16021 cgatcttgcg cggctcgagg aggaaacctc cgagcgcctc ggcaccacgg tcgagatccg
    16081 ctccggaaag aaaggcaccg gcaagctcgt gctccactac tcgagcctcg atcacctcga
    16141 cgagctgctc aagaagctga gatgaccgcg ctcagatgac gagtgagttg agctatactt
    16201 cgccgccttt gcgccgcgcc ttgggcaagc cgatcttgac cgtgctgaag tggcagttcg
    16261 cggcgacggt cttggccacg ctggctgcgg cgttgatcgc gggagttcac gccgctgctt
    16321 cggccgcggc cgggggcgca gtcagcatag tggcgggctt ggcgtcggcg ctggtcgccg
    16381 tgctgggcaa agggaactca gcagggggag tcctggttgg cgcgctgaga gccgaggcgc
    16441 tcaagatcgg cctcgcttta ctgctgctgt ggctcgtgct gtcgaactac ccgcaggtgg
    16501 cggtcggcgt ccttctgggc acgttcgtcg tgacgatgct gattttttcc atggcgtttt
    16561 tcgtacgcga atactgatgc aatcaggcga tggccgcagg taaagcgctc actccctcgg
    16621 agtacatcca gcatcacctg acgttcttca ccaagccggt gggagaaggc ggcggcttct
    16681 gggcgctcaa cctcgattcg atcgcggtgt cgatcctgct tgggctggtc ggcatgggat
    16741 tcatctggtg ggtggtgcgc ggcgccacct cgggagtgcc gggcaggcgc caggccgccg
    16801 tcgagtggct gatcgacttc gtcgattccc aggcgaaagg gatcttccag cacggcgacc
    16861 gccatcgctt cgtggctccg cttgcgctca cggtattcgt gtgggtcgtc ctgatgaacg
    16921 cgatggattt cctgccggcc gacctggtcg ggctggtgac gcacaggatc tccgaccacg
    16981 gcttccgcta cgtgcccacc gccgacgtca atacgacctt cgcgatgtcg atctcggtgc
    17041 ttttcctgac gctctacttc gccatctcgg tcaagggcgt gggcggctgg gtgcacgaac
    17101 tgttttgcgc gccgttcggc tcgcacccgc tgctgtggcc ggcgaacttt ctgttccaga
    17161 tggtggagta cgtgtcgaag accttgtcgc attcgctgcg gctgttcggc aacatgtacg
    17221 cgggcgagat cctgttcctg ctgctgtgga tgtgggcggc caccggcgtg gtcggaatca
    17281 tcttcggctc cttcctgggg ctgctctggg ccatcttcca catcctcatc gtgctgctgc
    17341 aggcctacat cttcatgatg ctgaccgtgg tgtacatctc gatggcgcac gagcaccact
    17401 gaccccacta cttcaagaaa gggaaaactc caatggaaaa actacagttg ctcgcgatgg
    17461 tccaggccta tacgggcatc ggcatcgggc tgatgatcgg cctgggcgcc gcgggcgcct
    17521 gcatcggcgt gggaatcatg tgcagccggt ttctggaagc cgccgcgcgc cagcccgaac
    17581 tcaccaactc gctgcagggc aaggtgttcc tgctgctcgg cctgatcgac gcctcgttca
    17641 tcatcggcct gggtatcgcg atgttcttcg ctttcgccaa tccgctggcc acggcgttcc
    17701 aatagggccg gtctaggagc ctcaaggcat gaacctcaac ctgacgctga ttgctcaggc
    17761 gatcaccttc accgtcttca tctgggtgac ggtgaagttc atctggccgc acatgctgcg
    17821 cgccatcgag gcgcggcaga agacgatcgc cgacgggctc gccgccgccg aggaaggcaa
    17881 gcgctcgctc gagcagtcga acaagcgcgc cgacgaggaa atcaggaaag cccgcgagcg
    17941 cgcgaccgag gtcctgtcgc aggcggaaaa gcgcgccgcg cagatgatcg acgaagccaa
    18001 gaacgtggcc aaggaggaag gcagccgcga aaaggccgca gccaaggccg agatcgcaca
    18061 ggaactgacc cgagcccgcg agcagctgcg cgaccaggtg gcttccctgg ccgtcgccgg
    18121 cgcagagaag atcctgcggc gcgaggtcga cgccaaggca cacggcgacc tgctcgattc
    18181 aatcaagaaa cagctctgat ggccgagccg acgaccgtcg cccgtcccta cgccgaagcc
    18241 gctttcaagc tggccgacgc cgagggcgcg ctcggcaagt ggtcggacat gctcggcgtc
    18301 cttgcgcagg tcgcggacga cgcccgggta cgcgcagcgg tcgccgatcc gaacctgtca
    18361 gacgccaagg ccgcgggctt gttcatcggg atcctcgccg gcaatctcag cggcgacgcg
    18421 gaaaatttcg tgcgcgtgct ggcggagaac ggccgactcg gtctgctgcc ggaggtccgc
    18481 gcccagttcg aggctctgaa gaacgagcgt gaaggcgtgc tcgaggccga agtgcactcc
    18541 gccttcgatc tgtcggaggc gcaggtcgcc gacctggtgg agcggctcga aaagaaaacc
    18601 ggccgcaagg tgagggcaaa aatcgcgatt cagaaggatt tgatcgccgg cgtgaggatc
    18661 gtgctcggcg acaaggtgat cgacggctcg gcgcgcgcgc agctcggcgc gctcgagacg
    18721 gcgttgaaag cataaaggag catcgatgca actgaacccg tccgagatct cggaactcat
    18781 caagagccgc atccagaacc tcgacgccgg cgcgcgcatg cgcacccagg gcaccgtggt
    18841 ctcggtgacc gacggcatct gccgcatcca cggcctgtcg gacgtgatgc agggcgagat
    18901 gctggaattc ccgaagggca cctacggcct cgcgctcaac ctcgagcgcg actcggtggg
    18961 cgcggtgatc cttggcgagt acacccacat caccgaaggc gacacggtca agaccaccgg
    19021 caagatcctt tcggtgccgg tcggccccga gctgctcggc cgggtggtga attcgctggg
    19081 tcagccgatc gacggcaagg gggcggtcaa cgccaagctc accgacgtga tcgagaaggt
    19141 cgcgcccggc gtgatcgcac gccagtcggt gtcgcagccg gtacagaccg gcctcaaggc
    19201 gatcgacgcg atggtgccga tcggccgcgg ccagcgcgag ctcatcatcg gcgaccggca
    19261 gaccgggaag accgcggtgg cggtcgacac gattatcaac cagaagggca aggacctctt
    19321 ctgcgtctac gtcgcgatcg gccagaaagc ctcgaccatc gcaaacgtag tgcgcaagct
    19381 cgaggagcac ggcgccatgg cctacacgat cgtggtggcg gcctcggctt ccgagtcggc
    19441 ggccatgcaa tacatcgcgc cgtactccgg ctgcaccatg ggcgaatact ttcgcgaccg
    19501 cggccaggac gcgctcatca tctacgacga cctgaccaag caggcctggg cgtaccgcca
    19561 ggtgtcgctg ctgctgcgcc ggccgccggg gcgcgaggcc tatcccggcg acgtgttcta
    19621 tctgcactcg cgcctcctcg agcgcgctgc gcgcgtgaac cccgcgtacg tcgagaagtt
    19681 caccaagggg caggtgaagg gcaagaccgg ctcgctcacc gcgctgccgg tcatcgagac
    19741 gcaagcaggc gacgtcacgg ccttcgtgcc gacgaacgtc atttcgatca ccgacgggca
    19801 gatcttcctc gaaaccgacc tgttcaacgc ggggatccgg cccgccatca acgccggcat
    19861 ctcggtgtcg cgggtcggcg gcgcggcgca aaccaagatc atgaagcgcc gcgacaccgg
    19921 cggcggcgtg cgcctcgcgc tcgcccagta ccgggagctc gccgcgttcg cgcagtttgc
    19981 gagcgacctg gacgaagcga cccgcaagca gctcgagcgc ggccgcatgg tcaccgagct
    20041 gatgaagcag gcgcagtacc agccgctgca ggtgtgggag atggcgctga cgctgttcgc
    20101 cgtgaacaac ggctacttcg acgacgtcga cgtgaagaag gcgctcgccg ccgagaagtc
    20161 gatgcgcgac tacgtcaagt ccaagttccc gatcatcgtg aagagcatgg aggacaagaa
    20221 ggacctgtcc cccgaggacg agaagtcgct gcacgaagcc atcaaggact ggaagaagaa
    20281 cggatcgttc tagatcatgc cgggcaccaa ggaaattcgg gtcaagatca agagcgtgca
    20341 aaacacgcgc aagatcacca aggcgatgga aatggtcgcc gcctcgaaga tgcgcaaggc
    20401 gcaggagcgc atgcgccatg cccgccccta cggcgagaag atccgcaacg tcgcggcgca
    20461 catttcgcac gccaacccgg aataccgcca tccgttcctg atcgatcgcg acaacgtgaa
    20521 gcgcgtcggg atcattgtca tcaccaccga caagggtctg tgcggtgcgc tgaacaccaa
    20581 cctcctgcgc atggtgctcg cgcagtacaa gcagtggcag gccgagggcg aggagatcga
    20641 ggtgtgcgcg ctcggcaaca agggcttcgg cttcatgcag cgcctcggcg ccaagatcgt
    20701 gtcgcacgcg atccagctcg gcgaccgccc tcagatggag aagctgatcg gcgcggtgaa
    20761 ggtgatgctc gacggctaca cctcgaaccg gttcgaccgc ctgatgctcg cctacacgcg
    20821 cttcatcaac acgatgaagc aggagccggt gatcgagcag ctcctgccgc tttcgggcga
    20881 gcgcctcggc gccccggaga ccgtgtggga ctacatctat gagcccgagg cgcgcgcggt
    20941 gctcgatcag gtgatgacgc gctatatcga ggcgcttatc ttccaggcgg tatcggaaaa
    21001 catggcctcc gagcagtcgg cgcgcatggt ggcgatgaag gccgcctccg acaacgccgc
    21061 cgacctgatc agcgagctca cgctcatcta caacaagaat cgtcaggccg gcatcaccaa
    21121 ggaactttcg gaaatcgtcg gcggcgctgc cgccgtctag aagaattcga ctccaggaaa
    21181 acgacatggc acaagcacaa ggcaccatcg tccagtgcat cggcgcggtc accgacatcg
    21241 agttcgatcg cgacagcatg cccgggatct acgacgcgct gatcctcgag gacgacccga
    21301 ccaacccgct ggtggagaag ggcctcacct tcgaggtcga gcagcagctc ggcgacggtg
    21361 tggtgcgcac catcgcgctg ggatcctccg atggcctgcg ccgcggcatg aaggtgaaat
    21421 cgagcggtgg gccgatctcg gtgccggtcg gcaagggcac gctcggccgc atcatggacg
    21481 tgctcggccg cccgatcgat gaagtcggcc cgatcaagtc cgacaagcgc cgctcgatcc
    21541 accagatcgc gccgaaattc gacgagcttt cgccctcggt cgagctgctc gagaccggca
    21601 tcaaggtgat cgacctgatc tgtccgttcg ccaagggcgg caagatcggc ctgttcggcg
    21661 gcgccggcgt gggcaagacg gtcaacatgc tcgagctcat caacaacatc gccacccagc
    21721 actcggggct ctcggtgttc gccggcgtcg gcgagcgcac gcgcgagggc aacgacttct
    21781 accacgagat gatcgaggcg ggcgtggtca agctcgacaa cctggaagag tccaaggtgg
    21841 cgatggtgtt cggccagatg aacgagccgc cgggcaaccg cctgcgcgtc gggctgagcg
    21901 gcctcaccat ggcggagcat ttccgcgacg aagggcgcga catcctgttc ttcgtcgaca
    21961 acatctaccg cttcacgctc gccggaaccg aggtgtcggc gctgctcggg cgcatgcctt
    22021 cagcggtggg ctaccagccg acgctggcgg aggagatggg ccgactgcag gagcgcatca
    22081 cctcgaccaa gaccggctcg attacctcgg tgcaggcgat ctacgtaccg gccgacgacc
    22141 tcaccgaccc ctcaccagcc acgaccttcg gccacctcga cgccaccgtc gttctctcgc
    22201 gcgatattgc gtcgctcggc atctacccgg cggtcgaccc gctcgattcg acctcgcgcc
    22261 aggtcgatcc caatacgatc ggcgaggacc actacaacac cacgcgcgcg gtgcaggcca
    22321 cgctgcagcg ctacaaggag ctgcgcgaca tcatcgccat cctcggcatg gacgagcttt
    22381 ccccggagga caagctcgcc gtggcgcgtg cgcgcaagat ccagcgcttc ctgtcgcagc
    22441 cgttcaccgt ggcgcagaac ttcaccggca cgccgggcaa gtacgtgccg ctcaaggaca
    22501 cgatcaaggg cttcaagatg atcgtcgccg gcgaatgcga cgcgctgccc gagcaggcct
    22561 tctacatggt ggggccgatc gaggaggcgt tcgagaaggc gaagaagctg tagtggctgc
    22621 gccgactatc cacgtcgatg tcgtgagcgc ggaggagagc attttctccg gcgaggccga
    22681 gttcgtcgtg ctccccggcg aggcgggcga gctcggaatc tacccgcgcc atacgccgct
    22741 cattacccgc atcaaggccg gggcggtgcg catcaagcct gccggcggcg gcgaggagca
    22801 gctgatcttc gtcgcgggtg ggattctcga agtgcagccg aaggtgatca ccgttctcgc
    22861 cgacaccgcg atccgcggcg ccgatctcga cgaagccaag gccaacgagg cgctgaagag
    22921 ggccgaggag gcccgcgcca aggcgaagga caaacaggag ctcgccacgg tagaaggcga
    22981 gctgtcgacg cttgccgcgc agctcgcggc gatccgcaaa ctgcgcaagc gctagcggtt
    23041 ctcgagccgg ttgtagtcga acagcccggc ttccagcggc gccgagcgct cgtacgcgtc
    23101 ggccatggca tcgctcgtca cccagaatgc cggatcggtg cggcgcacgg caaagcgatc
    23161 ggcgagcctg cggtagtcat cttcggaggc gagcgcaccg accgccgcgg cgagggccgg
    23221 cagttcggcg cgcgccacgc gccagatcgc attcggatag gcgccgatga accccggcac
    23281 gagggtcaaa gcgttttcgc cgggaagcag ccgcttgtcc tcgaagaaga ggttcgacac
    23341 gttgctgtgg gcggtgtcgc gcagcagggt gaaccaccgc ggcgcacgcg gcggatcgtc
    23401 cacgcgcaga aaggcgacct cgggcagcca cacggtgctt gcgccgcgaa ccgtgccgag
    23461 cgcttccagg tcgcgccgca gtgcccgatc ctcgatgctc gaaagatcga aacgcggcgc
    23521 gagcaccggt gcgagccgcg cgcgcagcag gccgtagagc tcgcgctgcg ggtcggcggt
    23581 gcggtagcgg atgcccgttt cgcggttgag ttgtgccttg cgtccgtaca cgtagtcctt
    23641 caccgagcgg gaggcgctgc gataccagtg atcacgcgtc gcttcgcgcg ccgcctcggg
    23701 caggaacacc aggaagttga actcgccttc catgcgaagg aaatccatgt agaggcgggt
    23761 gttcagctgg tggccggtgt tgccgtacac gtcgtagccg gcgacgagca ggtagtagat
    23821 gcgctcgaag agcgcatagc cgatcaccca tgcggtcttc ggcggctcgc cgaccaggcc
    23881 ctgaacgacc gaagcgctgt cgaagtgccg gaacacggtc aacgcggcat tgcgattgcg
    23941 cccgttgtcg ccgtcccaga cgagggacag gttgagcgcc cccggtgcag cgagcgagcg
    24001 ctccagggca cggtttttcg cctccagata gcgcgtctcg aggtcggcgt actgcagcca
    24061 tgggatgact accggcgcat tgctgcccca ctcggcgggc aggcgcagct cgtcggcctg
    24121 ccgcgcgatc agctcggcgc tcgcgtcgcc cgcgccagcc tgcggatcga cgaaaaacac
    24181 ccaaaagcgg tcctcgatca cgtcgagcgc gagctgcccg cgacagaccg ggcctttgat
    24241 gaagttcatg atgaagtact gcgcctcgtc gagcaggaag cggtagcgcg actcgaccgg
    24301 gatcgcggcg aatgcgacga acgggttgct ggccgactcg atgtcgtagc tgggaaaaac
    24361 ggaaacctcg tagggggcgg cgagaaacca ggcacggaat ttcgccatcc gctcgggcga
    24421 gagccggtag ggtaggtggg tcttggagag gattgcttcc tgttcaggca ccagccggta
    24481 gtaggcgagc ggcacaccgg ggtcgtcgta gggccggcgc gtcgcgatca gcgacaccgg
    24541 ctcgcccggc ggcgtggccg aacgcacgag gcggaacgct cgccgcgtcg tatcggtctc
    24601 gaagtagagg aatccgagga acagatgctc gtacaggtag cggctcatga gccgctcctt
    24661 cagcgagctg ccgttgagga acgcttccca tgcggccacc tgccgagtca gctcggccgt
    24721 cagcggcgcc ggcccgtcgt tgggcgcgcc cgccgccagc cagcgcgtaa gcgaaccgag
    24781 ctcgcgttcg tccagcccgg gcaggccgta gggcatgccg gcgagcggaa attcgcgctc
    24841 ataggcgtcg aacgcttcga cgcgcgggca ggactgcgcg cggtcgatgg agaaatcgaa
    24901 cggcttgggc agcacggcca cgtccggcag cggatgggcg cgtttgagag cgaggctgcg
    24961 ataaagaacg ctggccgcga ggttggcctc gggcgttggc gtgcgttcgt tcaggaccgg
    25021 gaagaagccc ttcttgcgcc attccgatgc gagcttcgca tcgacgaaca ggcgcgtcgg
    25081 cgcggcctgg cgcaggcggc tcccttggta gaccgcctcc gggttggccc cgcggacgac
    25141 gccttcccat gctgcgaggt tgagctggca cggcgcgtcg tagcaaccgt ggcacacgac
    25201 gcagcggcgc tccaggatcg gccgtacctc cgcgcggtag gacaccgcgc cgccaggctg
    25261 cgtgatcggg gcatcgtatc gggtcggatc cggggcgcca taacgctgat cgaaaagcga
    25321 gtctcgagtg agcgccgcgc agcttgccag gcagacggcg ccgacgacaa gcgcgtggcg
    25381 tgtggtcggc tgcaccgggc gatcataccg gcgtcgcctg cgcgggaagt agttgccgca
    25441 ctaaggctgt cgaaaaagct ctactgaaca aggtcgtgtc gttcttcgaa gactagtccg
    25501 gggggaccaa cttccgccct aacggcaacc acagggtgct gaaacaatcg ggtccaccgc
    25561 aggagattgc atgaaacata tcctcgactc cgactttcgc taccgcccga gcttcgatac
    25621 cgacgtgcgc aggacctttg agaaggtacg ccgtcaacag caggtgcgcg accagacgaa
    25681 gaccgaaccc gagatcaagg aggtcaaggt caaggtcctc aagctcgagc agctcaagaa
    25741 agccagcggc agtacctaag tcactcggcg agcgccacca cgacgtagcg cagcggaccg
    25801 tcggcgctga gcgcgtcgaa cggatagcag gtcaccaggg tcaggcgtgc ggggccttcc
    25861 tgcttcgcga cccacacttc acgccggtcg agcacgtcgg ccgcagccac gcggtaggag
    25921 acccgtgccc cgtcggcacg ctccaccgtc agcgcatcgc ccacctgcag cttgcgcatg
    25981 aacgcgagat gggtatcgcg gtggccgccg atcacgctat ttcccggccc gccgggaatc
    26041 gaggtaccgc gcacgtggcc cgggccgaac gccatgctgg cgccgtcggc gccggagagc
    26101 acgtagcgct ccacgccgag ccgcggcact gataggcggg ccacgggcca ggtatcggcc
    26161 cagccccacg gcttcacgtc tgccgcgcct tgcagcgttc ttgaccaggc atgtgcgatc
    26221 aggccctgcg ccacccacgc cttcgcctgg atccaggcgc cgcggccgaa ctgccaggtt
    26281 ccgatcacca gcagcacaac tgcaatcgcc ttcatgtgaa gcgccgcata accgccaggc
    26341 cgaggaggat ggcaagcgtg ccaagaagaa gcgcaagccg gctatccgtg gcgcctcgcg
    26401 gcagctcgcc gtagaccttg gcgtagtccc agccttcggg gaacaaggtc ggcatctcgg
    26461 cgagcttgag cgcctcctcg ggcgcgcgcg ccggggtctt gtcgattgcg acgaagctcg
    26521 tgtacttcgt gaccaggcga tgcgcttcgg cgagcgcgat cacccgcgcg cgcacctctg
    26581 cctccggtgc gccttcgcgc agcgaatcga tgagcgcgcc caccttttcg cgtgcccaca
    26641 ccgcgccgag cccttcggcg ttcgcattgt gtgccagtgg catgcgagcc tcccaggcgg
    26701 ttccgttgcg ctcgccctcg agccgcactt cgccctccag ccggtcgagc cgcgcgacga
    26761 gcacgaccgg ctcgccggca tacaggtcgg gaaggcgcgc cggccagacc tcgacggcga
    26821 cgccggaggg ccagtgagcc tgcacgccct tcaggaccgg ggtctcgagc ttggcgaaaa
    26881 gctcgcccat gcggctgcgg acctcgtcga cgcgcccgat gtaggtgaaa gtgccgcggc
    26941 cgagctgcgc cgctttggtc atgaaatggc tgttcggcgc cgagccgatg ccgatggtga
    27001 agaggcgaga gccgccgagc cgagcgcgga tcaggccgaa gagatgctct tcgttgccca
    27061 ccgcaccgtc ggtcaggaag atcacctggc gcacgtgctc tggatcgtcc gcgccgtcga
    27121 gcgcgagatg cagcgccgcc gccatctcgg tgccaccgcg cgcctcgagc gcgcgtaccc
    27181 agcgcgtggc cgcgcggaga ttgtccgggt cggccggccg ggccgcgtcg aacagcgcac
    27241 gcgcgaacga gttgaactcg atcacgttga accggtcctg cggctgcagc cgcgcgagcg
    27301 cgagctcgag cgcctccttg gcctggcgga tggaggcgcc gcccatcgag cccgacgtat
    27361 cgagaacgaa aatcacctcg cgcggcaggc gcgcgctgcg cggcgccgcc ggcggcagga
    27421 gcatgagcag gccgtagtgc ttgccgtctt tctcctcggt gaaccacgcc gcctggggca
    27481 gggacgcggg gcgcggcgac caggagagct cgaaatcgcg gttggccggc gtggtgccgc
    27541 ccgcaagcac gatctcgcgc cgcgaatcgc tcggcgcctt gacctcgatg gggtgatgcg
    27601 cgctttcgat cgcggcgagc ggtaccccgg catcgagcat cacctggatc gcgaccggat
    27661 tggtgcgctc gccctcggtg ccgggccgca tcaccggcgg ggtgatgcgc atcgcgtcgg
    27721 cgacttgcag cgacgcggcg ggtatgtagc gcggcccgac caccatcggg aagcgcagcg
    27781 agaagcgccc gctgtcgtag cgcaccacct gctggtattc gagctcgacc accacggttt
    27841 cacccgggcc gatgttcgcc acgctggtcg tgaagatgtt cggccgctcc tggtcgagca
    27901 gcgcggcgcg ctgcccggcg ccgcgcgcct gcgcgtaagc ctgtttcgcg acgttgcgct
    27961 cgcggatctc gccctcgacg atgcggctgc cgaccactag gcgcagggcg tcgaccgcac
    28021 cgctctgcgg gaggggaaag acgtagatgc cttcgtacca gacgtcgtgc ggattgcgga
    28081 aggtctgcac cacgcgcgct cgcgccaccg gcccggaaat ccgaaacaga acttcggtgc
    28141 caagcaacgg cgcggcgacc ggatcggccc cgcgcgcgcg caggtacagc atgccctcct
    28201 tcgtttcgga ctgcgaggcg tacgcgggcg cggcaaggag catcgccacg ccggcaagcg
    28261 cgatcgcgac cgcgacgccc agtgcggaag caagggcgag cagcgcgagc aggggaagcg
    28321 tcggtctggg atgtttttcg gtttccatgc gtccggcatt tgaagggcta aacccgcgcg
    28381 ccggccggac tcgcgcgggc aaagcggcga acaattctgg caaaccccgg gcagcgggct
    28441 tgggacccgg cgcgttgggc tatattcgcc gccatgtcgc gccgcatcgc gatcgtcgag
    28501 gacgagccgg ccattcgcgc caactacgcc gagggcctgc gcaagcatgg cttcgaggtc
    28561 gccgcctacg ccgggcgcgg cgaggcgctc acggcgatga ggagccggct gcccgacctc
    28621 gcgctgatcg acatcggcct gggcgccgac gtcgacggcg ggttcgcgct ctgccgcgag
    28681 ctgcgggcgc tgtcggcggc gctgccgatc atcttcctga ccgcgcgcga cagcgacttc
    28741 gacaccgtgg ccggcctgcg cctgggggcc gacgattacc tcaccaagga cgtgagcttc
    28801 ccgcacctgc tggcgcgcat cgccgcgctt ttccggcgcg ccgagctcgc aggcgatgcg
    28861 accgccgcgc ccgagcgcct ggaacgcggc gcgctgacga tcgacctcaa gcgcttcagc
    28921 gtagcctggc gcggcgcggc ggtgccgctc acgttgaccg aattctggat ggtgcacgcg
    28981 ctcgccaagt ttccggagca cgtgaagaac cgcgagcagc tgatgcgcga agcgaacctc
    29041 gtggtcgacg acggcacgat cacttcgcac atcaagcgca tccgccgcaa gttcctcgcg
    29101 gtcgacccgg cgttcgacgc catcgacacc gtgtacggca tggggtatcg ctggcaggca
    29161 tgaagcggcc gcgcttcagc ttgcgggtga agctcgcgct cgtgctgctc gccctggctg
    29221 cgctgccggt cgcggggatc ctgtacgtca acgaggtcga gaggttcctg ctacaggagc
    29281 aggagcagtc gctgctcgcc gtggcgcgcg ccgtcgctac cgccctgcac gagcggccgc
    29341 agctcctcgc cagcgcctcc gggcgcgacg acgaggtcga cgccatcctg ctcggcctgc
    29401 aacgcgcctc ctcgcgcatc tgggtagtgg atggccgcta ccaggtgatc gcccaggcgg
    29461 gcagcctgcg ccgcgcgacg gaggtggcct ccgaagcgcc ctggtggcag cccttatttg
    29521 tcaatacggt tgggtgggtc attccgcggc cgacggagga cttcaccgag ggcccgcccg
    29581 aggatctact cgccaccggc tcggacgtgg cggcggcgct gcagggcacg ccggccacgc
    29641 gtgcgcgcca tacgcgcgat ggccgcgccg tggtcctgtc ggcggcgcac ccgatctgga
    29701 ccggcgacca ggtcgccggc gccgtcgtga ccgaagagac gaccaacccg gtgatgtcgg
    29761 tgaagagcgc cgcgctcgag cgcctgctgc tcctcacgct ggcggcgttt gcgggcgcgg
    29821 cggcgctgct gatggcgttt gccacccgcc tttcgatgcg catccgcagg ctgcgcgacg
    29881 aagccgaatc ggcgatcgat gcgcacggcc gcatcgcgcg cctgacggcg tcgcaggccg
    29941 acgatgagat cggggacctt tcgcgcagct tctcggcggt gctcgagcgc ctcgcggagc
    30001 accatgccta cctggaagcg atggccgggc gcctgtcgca cgagctgcgc acgccgatcg
    30061 cagtggttcg gtcgtccttg gaaaacctga agatatcccg ggagaatcac gatctctaca
    30121 tcgatcgcgc cgagcagggc cttgcgcgcc tcggcaggat cctcgagcgg atgaccgaag
    30181 cctcgcgcct cgagcagagc ttgagcgcgg tcgagcgcag ccgctacgac ctggccgcgg
    30241 tggtgcgcgg ctgcgtcgag ggctaccgca tcgcgtatcc gaaggccgcg ttcgctctcg
    30301 aggtgcccga gcgccgggtc gaagtggagg gggcgcccga cctcgccgcg cagctcctcg
    30361 acaagctggt ggagaacgcg gtggacttct cgcgcggcgc ggcgccggtg cgcgtcgcgc
    30421 tcgaggaggc cggcggcgcc gccgcgctca cggtatcgaa caagggaccg ctgctgcccg
    30481 acaagatgcg cacgcgcctc ttcgaatcga tgatctcggt gcgcgacgcg tcgggcaccg
    30541 cgacgcccca tctcggcctg gggctttatg tcgcgcggct catcgcccag tttcacggcg
    30601 gcaccattgc cgcttcgaac ctcgcgaccg gcgacggcgt ggccctgggc gtgcgcattc
    30661 ctctggcatg gaaatgacct acgacctgct ggtggtcggc ggcgggatca acggcgccgg
    30721 catcgcccgc gatgcggcag gacgcggcct ttcagtgact ctggtggaaa aggcggatct
    30781 tgcgagcgcg acttcatcgt cctcatcgaa gctgatccac ggcgggctgc gctacctcga
    30841 gcacttcgag ttccgcttgg tggccgaagc gctcgccgag cgcgagatca tgctgcgcat
    30901 cgcgggccac ctcacctggc cgacgcggtt cgtcgcgccg catgttccgg agctgcggcc
    30961 gcgctggatg atccgggtcg ggcttttcct gtacgaccat ctcgctcggc gctcgctgct
    31021 gccggcatcg caggcggtgc gcctggaccg gccgccctac gcgtcggggc tgcggccgga
    31081 gctgaagcat ggcttcgtgt attcggactg ccgcgtcgat gacgcgcggc tggtcgtggt
    31141 gaacgcactg gatgcggcga atcgcggcgc gcgcatcctg gtgcgtaccg agtgcgtttc
    31201 ggcccggcgc gtcgaaggtg gctggcaggc acagctctcg aacggagaaa cggtgctcgc
    31261 gcgcgcgatc gtcaacgcgg ccggcccctg ggtgaaggag gtgctgaacg gaccgctcgg
    31321 cgagaccacg cgcgactccg tgcggctggt gaaaggcagc cacatcgtgc tgccgcgcct
    31381 ctatgagggc gagcacgcct tcatcctgca gaacgacgac cgccgcgtcg tcttcatgat
    31441 cccgttcgag gatcgcttca cgctcgtggg cacgaccgac atcgactacg acggcgatgc
    31501 cgcagcggtg gcggccagcg aagccgaggt cgactacctc tgccgcgcgg cgggccgcta
    31561 ccttgcgcgc gcgccagtac cgaaggatgt tctgtggcgc tacgctgggg tgcggccgct
    31621 gtatgacgac ggctcgcagg acccttcggc catcacgcgc gactacactc tgcgggtcga
    31681 cgacgcccaa ggcgcggcgc cggtgctttc ggtgttcggc ggcaagatca ccacctaccg
    31741 ctgcctggcg gaaagcgccc tggacaagct cgcgccctac tttccgggat tgaagccggc
    31801 atggaccgcg cgcgcgcagc tcccgggcag cgactttgcc gatcgtgaag caaccaagcg
    31861 cgagttgcag cagcgctatt cccaggttcc gccgaacgtg ctgcagggcg tgttccggcg
    31921 ccacgggacg ctcgcggcgc aggtgctggg tgacgggcgc ctcggtgagt acttcggtgc
    31981 cggactgacc gaacgcgagc tcggctacct cgtgaagcgc gaatgggcgc gcaccgcgga
    32041 agatgtgctg tggcgccgca cgaagtgcgg cctgcgcatg accgaggcgc agcgccgccg
    32101 tgtcgccgag gtgctcagcc gatgaagccg ctcgccgagc tcgatcgacg gggcgtgcgg
    32161 gccgtgctgc tggacatcga cgatacgttg acgaccgacg gcaagctcac tgccgggggc
    32221 tacggcgcgc tcgagcgcct gaagcacgcc gggctgcgcg tagttccggt gacagggcgg
    32281 cccgcgggct ggtgcgatca cattgcccgc atgtggccgg tcgatgccgt cgtcggcgag
    32341 aacggggcgt tctatttctt ccacgaggct aaccgcctgc agaagcggtt ccaggattca
    32401 gaagcaattc gtgccggcaa gcgcgtccgc ctgcaagcca tcgcggcgca gatcctcgcc
    32461 gcggtccccg gctgcgcgct cgcttccgac cagccttacc gcgcgaccga cctcgcgatc
    32521 gactattgcg aggatgtgcc gccgctgccc gtcgaggcgg cgcagcgcat cgccgcgctg
    32581 atgcgccagg ccggcctcac ggcgaaggtg agctcgatcc acgtgaacgg ctggttcggc
    32641 gaatacgaca agctcgccgc gacgcgcctt ctcttcgccg agcgcttcgg gctcgacctc
    32701 gcggccgtga atcgggaggt cgtattcgcc ggggattcgc ccaacgacgc gccgatgttc
    32761 gctttcttcg agaaatcggt cggcgtggca aacgtgcggc gcttcgaggg gctgctcgcc
    32821 gaagagccga agtacgtgac gcgcgccgcc tccggcgccg ggttcgccga gctcgccgag
    32881 cacttgctcc gtaggtgaat gcccgtcgcg ccgtcgcgac gcaagtcgga aagtcgtgac
    32941 gcgactgagc aggccggacc atccgatgag tcacccggct taacgaggcg cgcgattata
    33001 gtgacgcaga gaaaaccatg cgcgcgctcg tcttcgtcgc tttcctcgcc gccagcgcgg
    33061 cggcctgcgc gcagtctgcc gaatttgcgg cgctcgaccg caacggcgac ggcttcttga
    33121 gccgcatcga agccatcgcc gagcccgaaa tcgccaagcg cttcgcgcaa ttcgataccg
    33181 acaaggatcg gcggctgtcg cccgctgaat atcgggcggc gcgcgaggac aacgaacggc
    33241 gcgcggtgcg cgacgcggcg ctcgcggctc gagtcaagga agtgctggtc gcaacaagcg
    33301 gccttccgtc gaaggcgatc tcggtcgagg cctacgaagg cagcgtgcag ctttcggggt
    33361 tcgtgccggt gcccgacatg gcgtcgcgcg ccggccgcgt cgtcgccgcc gtgaacggcg
    33421 tgcgcagcgt ccacaacaac atcacagtca agtagactgc ggccccatgc cggggcccct
    33481 cgctggaatc cgtgttctcg acctgacccg cgtgctcgcg ggcccgtggg cggcgcaaaa
    33541 cctcgccgac ctgggcgcgg aggtgatcaa ggtcgagcgg ccgaagaaag gcgacgactc
    33601 gcgcgccttc ggcccgccgt ggctcaagga cgcgcagggc cgcgacacag ccgaatcggc
    33661 gtacttcgcg tgcgccaacc gcggcaagaa atcgctcacg ctcgaccttg cgcaaccgca
    33721 ggggcaagcg atcgcgcgcg agctcgccgc gcgctgcgac gtgctgctgg agaacttcaa
    33781 gtacggcgac ctcgaccggt acggccttgg ctacgcgcaa ctgaagaagg tcaatccggg
    33841 cctgatctat tgctcggtga ccggcttcgg tcataccggc ccgtggcgcg agcggccagg
    33901 atacgacttc atgatccagg gcatgggggg cctgatgagc atcaccgggg agcgcgacga
    33961 ccggcccggc ggcggcccgc agaaggcggg catcccgatc gccgacctga tcaccggcat
    34021 gtatgcaagc atcgccgtgt gcgccgcgct ggcgcatcgc gcgaagagcg gcgcaggcca
    34081 gcacctcgac ctcgcgctgc tcgattcgct ggcggcagtc ctcgcgaacc agggcgccaa
    34141 ctacctcgcc acgggcgagt cgcccggccg gctcggcaac gaccatccga acatcacgcc
    34201 ctaccaggtg ttcaagaccg ccgacggagc cgtcatcgtc gcgtgcggca acgacaacct
    34261 gtttcgcaag ttctgcgaag tcgcgggctg ccgcagcctc gccgacgatg cgcgcttcgt
    34321 gacgaacggc aagcgcatcg cgaaccgcgt cgagctgaca cgccttctgt ccgaaatact
    34381 agctcgacgt agtacgaggg aatggaccgc tgccctggaa gccgcgggtg tgccgtgcgg
    34441 cccgatcaac aatctcgagc aggtgtttgc cgagccgcag gccgtcgcgc gcgggctgaa
    34501 gatggagctg ccgcacccga cggcgggcaa ggtctcgctg gtgcgcagcc cgatgcgctt
    34561 ctccgaaacg ccgctgcagc acgaagtgcc gcccccgacg ctggggcagc acaccgacga
    34621 ggtgctgcgg gatcttctga agaagaaccc ctcggagatc gcacggctgc gctccgaggg
    34681 gatcgtctag cttctagttg tcgaccttcg cgccggcgtc cttcacgacc ttcgcccact
    34741 tcgcgatctc gctcttcacc agcttctcca tctccgccgg cggcatgccg cccgcgtctg
    34801 cgccttgctc ggcccagatc ctcttcaggt cgggctgctc catcgccttg acgatttcct
    34861 tgtacatgcg gtccttgatc gccggcgggg tgcccttgat cgcccagatc ccgtaccagg
    34921 tgcgcacctc gtagcccggc acgcccgctt cctgcaccgt cggcacgttc ggcagcaccg
    34981 ggttgcgctt gttgctggtg atagccagcg ggatcagctt gccggccttg acctgcgtcg
    35041 acgaggttcc catgccgtcg aaggcgaggt ccacctggcc ggcgaggagg tccaccatca
    35101 gcgggcccgc acccttgtag ggcacgtgta ccagcttcgt accggtcatg gtcttgaaca
    35161 gctcgaccga gagatggtgc gtggtgccgc ttccggccga gccgtagttc agcttgtccg
    35221 gatttgcctt ggcgtagtcg atcaggtcct tgagcgtctt gagggtgtcc ttgtgcttcg
    35281 ggtgcaccac gacgacgttc ggcacgaagg ccgcgaccgt gatcggctcg aagtccttct
    35341 ccaggctgta gttcagccgg gtgtagaggc tctcggcgat ggtatggtgc accgcgccga
    35401 agaaccagtt gtagccgtcg ggggcccgct tcgccgcatt ggcggcgccc acggtgccgc
    35461 cggcgccgcc ctggttctcg attagcgccg attcgccaag ctgctgcgtg agcttggccg
    35521 cgatcgggcg cgcgaacgta tcggttccgc cgccggccgg gaaagggttg aggaaaatca
    35581 tcggcttctg tggccactgc tgcgcttgcc ccgcgatgct ggtcaacgcc agtgccgcgc
    35641 tcgcaatcca cttcaaggtc ttcatctcct cctcctatgg ttgtgcggcg atgatatcag
    35701 gcagctacgg cagctccgct tcccaccagg ctgtcgattt ccgcgcgcga aaagccgaat
    35761 tcctccagga cttcgcgcgt atgctggccc aggacgggcg ccgggcgcga gaccttcccg
    35821 ggagtgcgcg acagcttgat cggtagccca agggcgcggg tgcgccctgc ccggggatgt
    35881 tcaagctcga ccaccatttc gcgcgccagc gcctgggggt cggccgccac gtcggcgatc
    35941 gaattgatcc tgccgcaggg cacgccttcg gcttccagcg cctcgatcaa gtcatccacc
    36001 ttgcggtcct tgagccgcgc attcatgagc ggcaccaatg cgtccaaatt cttcatccgg
    36061 tcggcgttgc tgcgaaagcg cgggtcttcc agccagtcgc gcgcgccgag cacgcggcac
    36121 atccgctccc agttcgcctg attggcgccg ccgatgttca gccagccgtc ggcggcgcgg
    36181 aacgcctgat agggcgccga gaggatgtgc gccgacccgg tagggcccgg cacttcgccg
    36241 gtggcgaagt ggatcgcagc ttgccagtag agctgctgga tcccggcctc gaagagcgac
    36301 gtatcgatga gctgcccttc gccggtcttg aggcggtgca cgtacgcgga cacgacgccg
    36361 agcgccccga gcaggccggc attgatgtcg cacacgggtc cgcccgactt caccggcgcg
    36421 cggccggcct cgccggtgac tcccatcagc cccgacatgc cctgggcgat gaggtcatag
    36481 ccgccctttt cggcgtacgg gccggtgcgg ccgtagccgg agatggagca atagatgatt
    36541 gccggattca ccgcgcgcag cgcctcgtaa cccagcccca gcttctccat cgtgcccttg
    36601 cggtagttct cggtgacgac gtcggagcgc gcggccatgc gcttgagcgc ttcctgcgcc
    36661 gtgggcagct tgaggttgag cgcgatgccg cgcttgttgc ggttcatcgc catgaacgac
    36721 gcggactcgc ccttgaccga gggccggtcc atcctgcggg tatcgtcgcc gccggggacg
    36781 cgctcgacct tgatcacgtc ggcgccgagg tcggcgagca tcaggccgca ggtcgggccg
    36841 gccatgatct gcgcgagctc caaaaccttc acgcccgcca gcggaccgct cattgctttt
    36901 cttaagcgac tttgcgggcc ggcgccgcag cggcgctcgc gaggtattcc atggcggcct
    36961 gcacgcctcc cttcttcacc ggcacaccgg caagcacgag ccccatctcg acgcccgcga
    37021 gcgtgcccat caacgtgaga tcgttgaaat ccccgagatg gccgatgcgg aacaccttgc
    37081 cgccgagctt ggtgagcccg gtgccgagcg acatgtcgaa cgcctcgagg atgattttcc
    37141 gcaggcgatc ggcgtcgtgc ccggccggca tgagcacggc cgtaagcgag cccgaatact
    37201 cgcgcggatc gagcgcgaac acctcgaggc cccacgcgcg cactgcgcgc cgggccgcct
    37261 cggcgtggcg ctggtggcgc gcgaacaccg cgtccagccc ttcctcgtcg agcagcatcg
    37321 tcagtgcttc gcgcaagccg tagagcaggt tcgtcgccgg cgtatacggg aagtagccgc
    37381 tcttgccgtt ggcgatcatc tcgtcccacg cccaatagct gcggggaagg cgcgcgctct
    37441 tgctcgcaga aatggccttc ggggagacgc agttgaagga cagccccggc ggcagcatca
    37501 gccccttctg cgagccggcg acggtgacgt cgacgcccca ttcgtcgtgg cgatagtcga
    37561 tcgaggcgag cgaggaaatc gtgtcgacca tgaagagcgc cgggtgcctt gccgcgtcga
    37621 tcgcttttct cacgtcggcg atgcgcgaaa ccacgccggt cgaggtctcg ttgtgcacca
    37681 cgcacaccgc cttgatcggc tggtcggtgt cggcgtgcag cttcttctcg atcgcctccg
    37741 gatcggcgcc gcggcgccag tcgctggcga tgaactcaac ctcgaggccg agcttggccg
    37801 ccatcttctg ccacagagtg gcgaaatggc ctgtctcgta catcagcacg cgatcgccgg
    37861 ctgagagcgt gttgcagagc gcggcttccc aggcgccggt gccggaggcg ggatagatca
    37921 ccacctcgcc cagcgtgctc ttgaagacac gcttcattcc ggcgagcact tcctttccga
    37981 gcgcggcgaa ctcggggccg cggtgatcca tcgtcgggaa atcgatcgcg cgcagcacgc
    38041 ggtcggggac gttggtcgga ccggggatct gcaggaagtg gcggccggcg cgtcggggca
    38101 ttgggttatt ctaccttcgg ctcatgaacg ctccattgac cccgatcaga ttcgccagcg
    38161 tcagccattc ctcgctggcg gcgcgcctgc gcaaggaact ggacgcggag gtcatgttcg
    38221 acgccgcgag ccgcggccgc tactcgaccg acgcctcgat ctaccaggtg gagccgatcg
    38281 gcatcgttgt gccgcgcagc gaagaggcgg cgcgcgccgc gatcgccatc gcggtcgatg
    38341 agggcgtgcc gatcctgccg cgtggcgccg gcagctcgca gtgcggccag gcggtcgggg
    38401 cggcgctggt catcgaccat accaagtact tgaacaagat attggagata aaaagggaac
    38461 aagccgtggc ggtcgtccag cccggtgtcg tgctcgacgc gctcaacgcc gagctgcgca
    38521 agcacggcct gtggttcccg gtcgacgtct cgaccagcgc gcaggcgacg ctcggcggca
    38581 tggcgggaaa caactcgtgc ggttcgcgct cgatcgccta cggcaacatg gtgcacaacg
    38641 tgctcgccat cgaggccgtc accgcttcgg gccagcgctg gcgcttcggc ccgctgagcg
    38701 atgcccgggg cgagtacggc gccttcgttc aaaagctgca gcaactctac gagcgtgaaa
    38761 aggcggagat cgaagcgcgc tttccgaagg tgctgagaaa agtcgcgggg tacaacctcg
    38821 accacctcgg gccgccgcac gcgaacgcgg cgcacttgct ggtcggctcg gagggcacgc
    38881 tcgcgtattc ggagcggctg catctcaagc tctcgccgct gccggcgaag cgctcgctcg
    38941 gcgtgtgcca cttcccgaag ttctacaccg cgatggagct cacgcagcac attgtgaagc
    39001 tgggcccgac cgcggtcgag ctggtcgacc gcaccatgat cggcctcgcg cgggagatcc
    39061 cggcgttcga gaaaaccgtc aacgcgttca tccgaggcga tcccgacgcg atcctgctgg
    39121 tcgaattcgc ggacgacagg gtcggaagac tcaaagacct ggtgcagctc atggccgacc
    39181 tcggccttcc cggcagcgtg gtcgagatcc cggacgagaa gctgcagaag cagctctggg
    39241 acgtgcgcaa ggcgggcctg aacatcatga tgtcgatgaa gggcgacggc aagccggtgt
    39301 cgttcatcga ggactgcgca gtgccgctcg agcacctcgc ggaatacacc gagcgcctga
    39361 cgcgcgtgtt cgagaagcac ggcacgcgcg gcacctggta cgcgcacgcg tcggtcggca
    39421 cgctgcacgt gcggccggtg ctcgacatgc gccgcgacgg cgccgagaaa atgcgcgcca
    39481 tcgccgaaga agcctgcgca atggtgaagg agtacaaggg cgcctattcc ggcgagcacg
    39541 gcgacgggtt ggtgcgctcg gaatggatcg cgccgttctt cggcccgcgc ctgaccgcgg
    39601 cgctcgccga gatcaagtcc tggctcgatc ctcgcggcct gatgaacccc ggcaagatcg
    39661 tccatccctc gcgcatggac gacaagtcgc tgtttcgctt caagcccggc tatgcgacct
    39721 cggttcccgc cacggtgctc gactggggcg aatggggcgg ctacgacaag gccgtcgaga
    39781 tgtgcaacaa caacggccac tgccgcaagt tcgacgccgg caccatgtgc ccctcgtacc
    39841 gcgcgacgat ggacgagcgc catctcaccc gcggccgggc gaatacgttg cgtcttgcgt
    39901 tatccaacca gctcggtgat cagggtgatg aagcagtaaa ggaagccctg gacctctgcg
    39961 tctcgtgcaa aggctgcaag cgcgagtgcc cgaccggcgt cgacatggcg cgcatgaaga
    40021 tcgagttcct cgcgcgctac aaggcgacgc atgggctctc ggcgcgcgac cgcgcgatcg
    40081 gctatctgcc gcgctacgcg ccttgggcag cgcgcttggc accagtatcg aatgctgttt
    40141 cttcgctgtt gaagtcttct ctcggttttg ccgccaagcg cgagctgccg aaatggcgca
    40201 gcgacgtttt cactgagcaa ggaagaaacg acggtgacgt agtgctcttt gtggacacgt
    40261 tcaaccggta cttcgagccg gagaatgcgc gcgcggcgct caaggtgctc gaggccgcag
    40321 gacatcgcgt gcacgctccc cgaggcctgt gctgcggccg caccttcctt tcggtgggac
    40381 tcgtcgacga agcgaagcgc gaggcccgga agatgctcga cgcgctctcg ccgtacgcgg
    40441 cgaagggaat tccgatcgtc ggg
//
LOCUS       DATBSZ010000124         7214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1452865, whole genome shotgun sequence.
ACCESSION   DATBSZ010000124 DATBSZ010000000
VERSION     DATBSZ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452865"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..756
                     /locus_tag="VIG70_08070"
     CDS             241..756
                     /locus_tag="VIG70_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY8250546.1"
                     /translation="MAWLIIPAVSALAAGVASAQGGGPSDPQIAGIVVTANQIDIDAG
                     KLAKSRSKNKEIQAFADQMITDHTAVNKQAVALANKLKVKPEDSDISKSLKEGASKNT
                     ANLQKLSGAAFDKAYIDHEVAYHQAVLDAIDKVLIPGAKNDELKALIVKVRPAIASHL
                     DHAKMIQGKVK"
     gene            765..1085
                     /locus_tag="VIG70_08075"
     CDS             765..1085
                     /locus_tag="VIG70_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEY8250547.1"
                     /translation="MLRRIGRAAALAALVLFSSLLLGARKDHRVSIEAMKYAPQSLEV
                     AVGDTVTWTNRDPVPHTVSAGKSVESGTIEGDREWKYVARQKGRVDYICRFHPGMQAT
                     LIVK"
     gene            complement(1089..2018)
                     /locus_tag="VIG70_08080"
     CDS             complement(1089..2018)
                     /locus_tag="VIG70_08080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY8250548.1"
                     /translation="MKAAFFMRHGGPEVFEYGDLPDPVPGAGEVLVNIHAASVNAADW
                     KMRAGSYAPVSHFPYVPGRDFSGVVAALGEGVKDFKAGDPVFGVCETSLEGAYAEKVA
                     IKASIVARKPDALSHAECAALALAGLTAVVSLEDTLQLARGETILIQGGAGGVAGFAI
                     QLAKHIGARVITTASAANHAYVRSLGADEVIDYRTEDFTRKVSGCDAVFDTVGDDVAL
                     KSFAVLRPGGRAAFIASGASAPASPRPDVRAYRPKVGRDRRHLERIVALVRSGAVRVP
                     EIRRYPLSEVAAAHAVSEGRHFRGKLVLEVRSG"
     gene            complement(2021..2935)
                     /locus_tag="VIG70_08085"
     CDS             complement(2021..2935)
                     /locus_tag="VIG70_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8250549.1"
                     /translation="MKLVCSLVLCAALSSVATAQPLVKEGKTVKISEHVFVIPDELAP
                     MVPNVGIVVGAKATLVVDPGMGLRSGETILREAAKLSRNTELYVVNTHFHPEHTTGEA
                     GFPPSAKIVRATAQQQDIEEMGMQWVQNFASRSPAIAEVLQGFTGFRKPAELFDKEKT
                     IDLGGVSVRLVRLGPGHTRGDTVFFVEQDRVLFSGDLVMSQLYPAFATPQSRVASWLA
                     SLDTLDAFRAQRLVPAHGALADSSAIGRYRDYLTALRARVAELKRAGKSAAEAAETVR
                     AEFPPRYPDWGQPLRVVAAANAIYAELP"
     gene            complement(2943..3905)
                     /locus_tag="VIG70_08090"
     CDS             complement(2943..3905)
                     /locus_tag="VIG70_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250550.1"
                     /translation="MRFLAALLFLGWSAVAPAQEAYPSRPIRLIVSFTPGGGADLTAR
                     TVGQSMSETLRQPVVVENRPGANGLLGATAVAKSASDGYTMLLTDRGALGVNPSLYKS
                     LPYDPLRDFEYVGIVTSAPYVLVIDPKLPAKSLAELAQLAKARPGSINYASFGVASMA
                     QLNIEALKARLGIDLTHVPYKGAGPAVQGVVAGEAGVTIASPAAVLGFVRDGRLRALA
                     IGAPSRSTLLPEVPTLLEAGGDEDTLVPTYFAFALPAGTPRPIVDRLHEAMKRALAAP
                     EVAARLGAAGLEPTGGTGEALRELVRRDIPRFRKIIQDVGIQPE"
     gene            complement(3913..4857)
                     /locus_tag="VIG70_08095"
     CDS             complement(3913..4857)
                     /locus_tag="VIG70_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250551.1"
                     /translation="MALVSAFATSHAYTFQEPETWDQRRERSKASVARKAGRPATDTR
                     EARAETLDDNRSRYAHIRTAHQTIRERLARASADAVLLVGDDQTENFSADNLPQLLVY
                     TGPDYVADDWDRKKTARVSSHPEIARLLVEGCLDEGFDVGYSVAFKDGKLVSHAHVEP
                     ILYLVRESGTPVVPVFVNAVHPPAPSAARCYAFGQALRRVADRRLAGRRIILCASGGL
                     SHFSPSHPWEHHVGSRYVGDISVEFDRRIVEWMRAGEGHRLAALSAPELIEHGQAELR
                     QWIVLLGAVGAQKPEFLVYEPLFRSIMGMGVGWWNMDQ"
     gene            complement(4870..5778)
                     /locus_tag="VIG70_08100"
     CDS             complement(4870..5778)
                     /locus_tag="VIG70_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8250552.1"
                     /translation="MLRYGQAASAHKSIYSLPIALAESEGLFRREGLDFRVVIPIPGG
                     ADLMVAALHDDTADVAHVATPFLVRAALAGSDAVAIAAEFNDPIYSLVARPEIARIAD
                     LKGKVVGMADPDGSIAYSMRKLLALHGMSEKDLRVRIIGGTPARLACLKRGECAAVPL
                     GQPQDLVAQSEGYRLLGASNDAVPAYLYTVTAARRSWAAANQDAVVRYVRALAASFAT
                     IRDPAKRGAVVNTIVEVTGVEPAVAERVLALYGGKNVLPQRGEIDLKGLAQVIAFMGE
                     TGQLKSPLPAPQRFADLRYLEAAGIR"
     gene            complement(5829..6026)
                     /locus_tag="VIG70_08105"
     CDS             complement(5829..6026)
                     /locus_tag="VIG70_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250553.1"
                     /translation="MGTTTGRMTGACRVRGFKKDVSPEEVVISCSKDGYRQIRVFRYP
                     LPKGKPVKSVETECRLQREKS"
     gene            complement(6129..>7214)
                     /locus_tag="VIG70_08110"
     CDS             complement(6129..>7214)
                     /locus_tag="VIG70_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250554.1"
                     /translation="HNAMFDAKGRVWIAATVRDRDNPAFCKQGSAHPSAKVFPLPQSS
                     RQVAMLDPKTMKYSFIDTCFGTHHPQFGYDADNTLWLSGTGPVAGWVNTRVFEETGDA
                     AKAQGWSPFVLDTNGNGRRDEYVEPNQAIDPAKDKRIIPGSGPYAVMPSPADGSIWYT
                     VGVFGGAPGFLRFDPKTQLSEVYLVPAPAYGIRGGDIDKSGVLWGSGSSGHLVSFDRR
                     KCKAPLNGPKATGGHCPEGWSFYQYPGPGFRGLGENSAESSYYTWVDQHNTLGLGENI
                     PMSTANLNDGFVALKDGKMILLRIPYPLGFYAKGFDGRIDDPNAGWKGRGLWSTNGDR
                     TPWLMEGGKGSKPRAVHIQLRPDPLAR"
BASE COUNT         1184 a         2510 c         2376 g         1144 t
ORIGIN      
        1 cctgttctac ggtccgcgct gggtgaagcc cggccgggtc gacgcgcggg tcgaggtcgc
       61 ggacatcgcg ccgacgctcg cgcgcatcct cgggattccg gcgccgtcct cgtcggaagg
      121 caagccgctg ccgctcatgc cctgagggct tgtcgaaagt cgggcgcacc cgggaacgcc
      181 gggagccgcc ggagcatctt atgccagctt cctatcatca aaggagaaca gtccgtgaag
      241 atggcatggt taatcatccc cgcagtttcc gcgctcgccg ccggcgtcgc gtcggcccag
      301 ggcggcgggc cgagcgatcc gcagatcgcg ggcatcgtag tgaccgcgaa ccagatcgac
      361 atcgacgccg gcaagctcgc gaagagccgc tccaagaaca aggagatcca ggccttcgcc
      421 gaccagatga tcaccgacca taccgcggtg aacaagcagg cggtcgcgct cgcgaacaag
      481 ctgaaggtca agcccgagga cagcgatatc agcaagagcc tcaaggaagg cgccagcaag
      541 aacaccgcga acctgcagaa gctctcgggc gccgcgttcg acaaggccta catcgatcac
      601 gaggtcgcct accaccaggc ggtgctcgac gcgatcgaca aggtcctaat tcccggcgcg
      661 aagaacgacg agctcaaagc gctgatcgtc aaggtgcggc cggcgatcgc gtcgcacctg
      721 gatcatgcca agatgatcca gggcaaagtc aaatagcgca gccgatgctg cgtcggatcg
      781 gacgggcggc tgcgcttgca gccctcgtcc ttttctccag cctgctgctc ggtgcgcgca
      841 aggaccaccg cgtgtcgatc gaggcgatga aatatgcgcc ccagtcactc gaggtcgccg
      901 ttggcgatac cgtcacctgg acgaaccgcg atcctgtgcc ccacacggta agcgcgggca
      961 agtcggtcga gtcaggcacg atcgagggtg atcgagagtg gaagtacgtc gcgcgccaga
     1021 aggggcgcgt cgactacatc tgccggttcc atcccggcat gcaggccacg ctgatcgtga
     1081 aatagcgcct agccggagcg cacttccagc accagcttgc cgcggaagtg gcggccctcg
     1141 ctgacggcgt gcgccgccgc aacttccgac agtggatagc gccggatctc gggaactcgc
     1201 acggccccgg agcgcacaag cgcgacgatg cgttcgagat ggcggcggtc gcgccccacc
     1261 ttcgggcggt aggcgcgcac gtccgggcgc ggcgaagccg gcgccgacgc gcccgaggcg
     1321 atgaacgcgg ctcggccgcc gggccgcagc acggcaaacg acttcagcgc cacgtcgtct
     1381 ccgacggtgt cgaacaccgc gtcgcagccc gagaccttcc gcgtgaaatc ttcggtgcga
     1441 tagtcgatca cctcgtccgc gccgagcgag cggacatagg catggttcgc cgcgctcgcg
     1501 gtggtgatga cgcgcgcgcc gatgtgcttc gcgagctgga tcgcgaagcc cgcgactccg
     1561 cccgctcctc cctggatcag gatcgtctcg ccgcgcgcca gctgcagcgt atcctcgagc
     1621 gacaccacgg ccgtcaaacc agccagcgcg agcgccgcgc attccgcatg cgaaagcgcg
     1681 tcgggcttgc gcgccacgat cgaggccttg atcgcgacct tttccgcgta ggccccctcc
     1741 aggctcgtct cgcagacgcc gaacaccgga tcgcccgcct tgaagtcctt caccccctcc
     1801 cccagcgcgg cgacgacgcc ggagaagtcc cggcccggca cgtacggaaa gtgcgaaacg
     1861 ggtgcatagc ttccggcgcg cattttccag tcggccgcat tgacgctcgc cgcatggatg
     1921 ttcaccagca cctcgccggc gccgggcacg gggtcgggga gatcgccgta ctcgaagact
     1981 tccggaccgc cgtgccgcat gaagaaggcg gctttcacgc ttacggcagc tccgcgtaga
     2041 ttgcgttcgc cgcggcgacg acccgcagcg gctgacccca gtccggatac cggggtggaa
     2101 attcggcgcg cacggtctcg gctgcctcgg ccgcggactt gccggcgcgc ttgagctcgg
     2161 cgacgcgcgc gcgcagcgcc gtcaggtagt cgcgatacct cccgatggcc gaactgtccg
     2221 cgagcgcgcc gtgtgcaggc acgaggcgct gtgcgcggaa cgcgtcgagc gtgtcgaggc
     2281 tcgcaagcca cgacgcgacc cgcgattgcg gcgtagcgaa cgccgggtag agctggctca
     2341 tcaccaggtc gcccgaaaag agcacccggt cctgctcgac gaagaacacc gtgtcgccgc
     2401 gggtgtgccc cgggccgagg cgcacgagcc gcacgctcac gccgcccagg tcgatcgtct
     2461 tctccttgtc gaagagctcg gcgggcttgc ggaacccggt gaagccctgc agaacctcgg
     2521 cgatcgcggg cgagcggctg gcaaagttct gcacccactg cattcccatc tcctcgatgt
     2581 cctgctgctg ggccgtggca cgtacgatct tcgcgctcgg cgggaagccc gcctcacccg
     2641 tcgtgtgctc ggggtggaaa tgcgtattca ccacgtagag ctcggtgttc cggctgagct
     2701 tggccgcctc gcgcaggatg gtctccccgc tgcgcaagcc catccccgga tccaccacca
     2761 gcgtcgcttt cgcaccgacc acgatgccga cgttcggcac catcggcgcg agctcgtcgg
     2821 gaatgacgaa cacgtgctcg gagatcttta ccgtcttgcc ttccttgacc agcggctgcg
     2881 ccgtcgcaac ggagctcagc gcggcgcaca ggacaagaga acacacgagc ttcatgggga
     2941 cctcattcgg gttgaatgcc gacgtcctgg atgatcttgc ggaacctggg aatgtctcgc
     3001 cggacgagct cgcgcagcgc ctcgcccgtg ccgccggtag gctcgaggcc cgccgcgccc
     3061 aggcgcgcgg ccacttcggg cgccgcgagc gcgcgcttca tcgcctcgtg gaggcgatcg
     3121 acgatcgggc gcggcgtccc ggctggaagc gcgaaggcga agtacgtggg caccagcgtg
     3181 tcttcgtcgc cgcccgcctc gaggagcgtc ggcacttccg ggagcagtgt cgagcggctc
     3241 ggcgcgccga tcgcgagcgc gcgcagccgt ccgtcgcgca cgaagccgag cacggctgcc
     3301 ggcgaagcga tcgtgacgcc cgcctctcct gcgaccactc cctgcaccgc cggcccggcc
     3361 cccttgtaag gaacatgcgt gaggtcgatc ccgagccgcg ccttcagagc ctcgatgttg
     3421 agctgcgcca tgctggcgac gccgaagctc gcatagttga tgctgcctgg ccgcgctttt
     3481 gcgagctgcg caagctcggc gagcgatttc gccggcagct tcggatcgat caccagcaca
     3541 tacggcgcgg aggtgacgat cccgacgtac tcgaaatccc gcagcggatc gtagggcagg
     3601 ctcttgtaca ggcttggatt gacgcccagc gcgccgcggt cggtgagcag catcgtgtag
     3661 ccgtcggagg ccgatttcgc caccgccgtg gcgccgagca gcccgttcgc gccgggacgg
     3721 ttctcgacca ccaccggctg gcgcagcgtc tcggacatgc tctgccccac cgtccgcgcc
     3781 gtgaggtccg caccgccgcc gggcgtgaaa gacacgatca ggcggatggg tcgcgaggga
     3841 tacgcttcct gcgccggcgc gactgcgctc catccgagaa agagcaatgc agccaggaat
     3901 ctcatcggtc cctcattggt ccatgttcca ccagcccacc cccatgccca tgatgctgcg
     3961 aaagagcggc tcgtacacca ggaactcggg cttctgtgcc ccgaccgcgc cgagcagcac
     4021 gatccactgg cgcagctcgg cctggccgtg ctcgatcagc tccggcgcgg aaagcgcggc
     4081 gagcctgtgg ccctcacccg cgcgcatcca ctccacgatg cggcggtcga actccacgct
     4141 gatgtcgccg acgtagcgcg agccgacatg atgctcccac gggtgcgacg gcgagaaatg
     4201 cgacaggccg cccgaggcgc agagaatgat ccggcgcccc gcgagccgcc tgtccgccac
     4261 gcgccgcagc gcctgcccga acgcgtagca gcgcgccgcg gacggcgccg gtggatgaac
     4321 cgcgttcacg aacaccggca ccaccggcgt gccgctttcc cgcacgagat agaggatcgg
     4381 ctcgacgtgg gcgtgcgaca cgagctttcc atccttgaac gccaccgaat atccgacatc
     4441 gaagccctcg tcgaggcagc cctcgaccag caggcgcgcg atttccggat gactggaaac
     4501 gcgcgccgtc ttcttgcggt cccagtcgtc ggcgacatag tctgggccgg tgtagacgag
     4561 gagctgcggc aggttgtcgg cgctgaaatt ctcggtctga tcgtcgccga cgagcagcac
     4621 cgcatcggcg cttgcgcgtg ccaggcgctc gcggatggtc tggtgcgcag tgcggatgtg
     4681 cgcgtagcgg ctccggttgt cgtcgagcgt ttcggcgcgc gcctcgcgcg tatcggttgc
     4741 cggccgcccc gccttgcgcg caacgctcgc tttggagcgc tcgcgccgct ggtcccaggt
     4801 ctccggctcc tggaacgtat aggcgtgcga agtggcaaaa gcagaaacga gggccatgcg
     4861 ttcagcacct taccggatgc cggcggcctc caggtagcgc aggtccgcaa agcgctgcgg
     4921 cgcggggagc gggctcttga gctgtccggt ctcgcccatg aaggcgatca cctgcgcgag
     4981 gcccttgagg tcgatctcgc ctcgttgcgg cagcacgttc tttccgccat agagcgccaa
     5041 gacacgctcg gcgacggcag gctccacgcc ggtcacttcg acgatcgtgt tcaccaccgc
     5101 gccgcgcttc gctggatcgc ggatcgtcgc gaacgacgcg gcaagcgcgc gcacgtagcg
     5161 cacgacggca tcctggtttg ccgccgccca tgaccggcgc gctgccgtga ccgtgtagag
     5221 atacgcaggc acggcgtcgt tcgaggcgcc gagtagccga tagccttcgc tttgcgcgac
     5281 caggtcctgc ggctgcccga gcggcaccgc cgcgcactcc ccgcgcttca ggcaggcgag
     5341 gcgcgccggc gttccgccga tgatcctcac gcgaaggtcc ttctcgctca tcccgtgcag
     5401 ggcaagcagc ttgcgcatgg agtacgcgat gctgccgtcc ggatcggcca tgccgaccac
     5461 cttgcctttc aggtcggcga tgcgcgcgat ctcgggacgc gcgaccagcg aataaatcgg
     5521 atcgttgaac tctgcggcga tcgccaccgc gtccgaaccg gcgagcgcgg cgcgcacgag
     5581 gaacggggtc gcgacgtgcg ccacatcagc ggtgtcgtcg tgcagcgcgg cgaccatcag
     5641 gtccgcgccg ccggggatcg gaatcacgac gcggaaatcg agcccctcgc gccggaacag
     5701 gccttcgctt tcggcgagcg cgatcggcag cgaataaatg ctcttgtgcg ccgatgccgc
     5761 ctgtccgtag cgcagcacca ggttttgccc ccaggaaacc cccgggagga aaactagggc
     5821 aaggacgcct acgatttttc gcgctgcagg cggcattcgg tttcgaccga cttgaccggc
     5881 ttgcctttcg gcagcgggta cctgaagact cggatctgcc ggtagccatc cttcgagcac
     5941 gaaatcacga cctcttcggg gctgacgtcc ttcttgaagc cgcgcacccg gcaggcgccg
     6001 gtcatccggc ccgtcgtcgt gcccatccag agcgtcgggg cccctggtgg ctaatcgcgc
     6061 cgagcagcgc gaccgcgaac agcccatgca cgatcagcag gactgtttgc cagttcactg
     6121 cgccttccct atctcgcgag cggatcgggg cgaagctgga tgtgcaccgc gcgcggcttg
     6181 ctgcccttcc cgccttccat cagccacggc gtacgatcgc cgttggtgct ccacagcccg
     6241 cgtcccttcc agccggcgtt cggatcatcg atccgcccat cgaagccctt ggcgtagaag
     6301 cccagcggat acgggatgcg cagcaggatc atcttgccgt ccttcagcgc gacgaagccg
     6361 tcgttcaggt tggcggtcga catcgggatg ttttcgccga gcccgagcgt gttgtgctgg
     6421 tcgacccacg tgtagtagct cgactccgcg ctgttctcgc cgagcccgcg gaagcccggt
     6481 cccgggtatt gatagaacga ccagccttcg gggcagtgac cgccggtggc cttgggtccg
     6541 ttcagcggcg ccttgcactt gcgccggtcg aagctcacca ggtgcccgct cgagcccgat
     6601 ccccacagca cgccgctctt gtcgatgtcg ccgccgcgga tgccgtacgc cggcgccggc
     6661 acgagataga cctcggagag ctgcgtcttc ggatcgaagc gcaggaatcc cggcgcgccg
     6721 ccgaacacgc cgaccgtgta ccagatcgag ccgtccgccg ggctcggcat gacggcgtac
     6781 ggacccgagc cgggaatgat gcgcttgtcc ttggcgggat cgatggcctg gttgggctcc
     6841 acgtactcgt cgcgccgccc gttgccgttg gtgtcgagca cgaagggcga ccagccctgt
     6901 gctttcgccg catcgccggt ctcctcgaat acgcgcgtat tgacccagcc ggccaccggg
     6961 ccggtgccgc tcagccacag cgtgttgtcg gcgtcgtagc cgaactgcgg atgatgcgtg
     7021 ccgaagcagg tatcgatgaa gctgtacttc atggtcttcg gatcgagcat cgccacctgg
     7081 cgggaggact gcggcagcgg gaacaccttg gccgagggat gcgcagagcc ctgcttgcag
     7141 aatgccgggt tgtcccggtc gcgcaccgtc gccgcgatcc atacccgccc tttcgcgtcg
     7201 aacatggcgt tgtg
//
LOCUS       DATBSZ010000125        20191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_154070, whole genome shotgun sequence.
ACCESSION   DATBSZ010000125 DATBSZ010000000
VERSION     DATBSZ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20191
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154070"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VIG70_08115"
     CDS             complement(<1..881)
                     /locus_tag="VIG70_08115"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250555.1"
                     /translation="MTRFIAFALVLVASGAAAQSFPSKPIRVIIPFVAGGSSDIVGRA
                     IASKFQELLGQAGVVENRPGANGAIAAEFVAKAEPDGHTILVGSIGVFSINAALFKDL
                     RYQPVRDFAPITLAVTTPNVLITKPTLAAASLKELVDHMKKNPGKLSYCSSGTGSSDH
                     LTAELLKQASGTDAVHVPYKGGAACQTDIMGNQVDFSFQNLGAVTNYIKGKRMKALVV
                     TAKARHPQLPDVPSAGEAGFADLVVTSWQAAAAPAKTPKDVVTRLHGAMVKALRSPDI
                     AERMNQIGFDVVASSPE"
     gene            955..1932
                     /locus_tag="VIG70_08120"
     CDS             955..1932
                     /locus_tag="VIG70_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250556.1"
                     /translation="MRALFLVVVSFFSAAVALHIPGALAQPYPGKPIRIIVPYAAGGT
                     SDILARQIGPKLTDAWGQPVIVENRPGANGNVGADFVAKSAPDGYTLLLTDLGGLVIS
                     ASVYPKLPFNPSKDFAPVVMVSYSPHVLAVNPEVQVKNVKELVAMAKAYPGKLNFAIS
                     GIGGAPQLAGIEFAQRMGIDWTYIPYKGGADAITGVVGGQAHVLFNGMLATWPHVTGG
                     RLKALAISSAQRMPSAPDTPTVAEQGLPGFETGSFQGVVGSRGTPRETVAKLNAELVK
                     VLNTAEMKERFARQGTDVRTGTPEALGRWMESEQAKWAKVVKDSGAKFD"
     gene            1925..3649
                     /gene="araD"
                     /locus_tag="VIG70_08125"
     CDS             1925..3649
                     /gene="araD"
                     /locus_tag="VIG70_08125"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HEY8250557.1"
                     /translation="MTSRKKPEELRSHRWYGADDLRAFGHRSRTAQMGYDRSDYAGKP
                     VIAIINTWSDINTCHTHFKQRVEEVKRGVWQAGGFPVEMPAMSLAEPMQKPTTMMYRN
                     FLAMETEELLRSYPADGAVLMGGCDKTTPALIMGAISMNLPSIYMPAGPMLSGHWRET
                     TLGSGSDSWKYWAELRAGTITRKDWNEIEDSIARSPGTCMTMGTAATMMSLAESLGFS
                     LPGAASIPAPDSNHAKMATLTGKRIVDMVWEDLKPRDFLTRESFDNAAVTLMAMGGST
                     NALIHLVAMAGRAGIRFPLERFNEFSAKTPLLANVRPSGDKFLMEDFYYAGGLRALLA
                     ELKDLLNLDCRTVNGKTLGENLQGASIYNEDVIRKRSNPLKASGGLVVLRGTLAPNGA
                     VIKAAATQILKHVGKAVVFDDYNDMAARIDRDDLDCDEDSVLVLRNAGPLGGPGMPEW
                     GMLPVPKKLLKQGVRDMVRISDARMSGTSYGCCVLHVAPESYVGGPLALAKSGDLIEL
                     DVEKRVLNLKVADAELAKRKSAWKPPVRKYERSYGAIFAQHVKQADQGCDFDFLEGTA
                     AVPEPEIH"
     gene            3649..4416
                     /locus_tag="VIG70_08130"
     CDS             3649..4416
                     /locus_tag="VIG70_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HEY8250558.1"
                     /translation="MEMPQNAFKRALRAGKPQIGLWSSLSSNYTVEVIAGAGFDWILL
                     DSEHSPADIESLLTQLQAAAAYPTHPVVRVPWNDMVNMKRVLDIGAQSLLIPYVQNAA
                     EARSAVAHTRYPPAGVRGVAGTTRATRFGRIKDYAHRAHEEICVLVQVETQPALDNIE
                     AICEIDGVDGVFIGPADLHASMGHAGEVANPKVKPLIDDAIRRIRKSGKAPGILTPNE
                     ADARHWLECGALFVAVGADVGILARGAEALAAKFKKP"
     gene            4413..5351
                     /locus_tag="VIG70_08135"
     CDS             4413..5351
                     /locus_tag="VIG70_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEY8250559.1"
                     /translation="MKPEIIITAKGHAGTMATLQSEFTCNHLFEAAEKAPFFEKCAPR
                     VRGLATFGPMPVDGKLMDALPKLEIISNFGVGVDAINLDDAKKRKIIVTNTPDVLNEC
                     VADTALALVMNTVRKFPQSERYLRAGNWASRGPYPLTTSLGGKTLGILGLGRIGEAIA
                     KRAAACGMKIRYHNRNRKDVPYPYDPDPATLAKNADVLIVVTPASAETNRIINEKILQ
                     ALGPESYLVNIARGSVVDEPVLLRYLQEKKIAGAGLDVYADEPRVPPEFFTLENAVLF
                     PHVGSATVETRKAMGDLQIENLRRHFAGKPVVTRVV"
     gene            5440..5583
                     /locus_tag="VIG70_08140"
     CDS             5440..5583
                     /locus_tag="VIG70_08140"
                     /inference="COORDINATES: protein motif:HMM:NF022103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEY8250560.1"
                     /translation="MNRPPKEEPKRISSQALLEGRRELVILHNGREYRLRLTQNGKLI
                     LTA"
     gene            5659..5889
                     /locus_tag="VIG70_08145"
     CDS             5659..5889
                     /locus_tag="VIG70_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250561.1"
                     /translation="MHRNDPEESRVETITAALVYLMTHYARTGCPRLAVCVSRHLQAL
                     ALHPDAAPVLRDICAALHGAWANAAADDPQIH"
     gene            5922..6860
                     /locus_tag="VIG70_08150"
     CDS             5922..6860
                     /locus_tag="VIG70_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY8250562.1"
                     /translation="MKFQKTLVAAAVGLLAAPAAAQQVTFMTGPQGGSWIPLGGALKG
                     MWEKAVPGLSITSTPGAGIANVRGVDEGKAQIGLANSNTTVDGLEGRAPYPKKVTKVC
                     QLANLYPQYFQVVALSDAKINSYADLKGKALVTQPKGNTAEMLTEMILKVNGLSYQSL
                     SKTNFQAAYTDAVSMMKDGHVQVFTLGTTAPASAVMDLASARDVKLVPVDDKTFNAVK
                     KANPGHNKLIIKAGTYPKQDKDVPVIGYSTHLVVACDLPENTVYQMVKTMAANVDSMA
                     AVVKSIEGMKPKDMAQDIGVPFHKGASKFYKEAGAL"
     gene            6870..8798
                     /locus_tag="VIG70_08155"
     CDS             6870..8798
                     /locus_tag="VIG70_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEY8250563.1"
                     /translation="MEEEKPQTAPQGGALKVAVALIAIAMSLYHMHAAAFGPPEALIF
                     RGTHLLFAVTLVFLLYPTRPGGGLGWRALDAVLMLAGWGIVLHIFFNYQYYTNRIIYI
                     DELTFQDKFWAVAAILIVLEGTRRVLGWALPLTAVAFLAYAVSTQVTFPVLLEQVYLS
                     TEGIFGSTLGVSASYVMMFVLFGAFMEKSGTGQLFMDFSMSITGRYAGGPGKVAVVSS
                     SLFGTVSGSAVANVMVDGPITIPLMKRTGFRPPFAAAVEAVASTGGQLMPPIMGAAAF
                     VMAEFLAVPYAQITVWAILPAFLYYLAVFAAVHFEAKRYKLAGVPASELPRLGNVMLT
                     RGHLFIPILLVLTGLIYGYSAPLCALVGALSCLPLALLKRATRTGISWRSVLEALEEG
                     AKNTLAVAMACAAAGIVIACVTITGLGIVFTQVVVALAQDTLILALILTAMAGIILGM
                     GMPTTPAYIMMAALLIPALIKLGIAGCPVPVTPECKAAVIPAAHMFAFYFAILSAITP
                     PVALAVFAAASLAKANMWQSGFAAMRAAAPAYIVPFMFVYEPLLLLIVKDWSVEWLFV
                     VWSVITASIGVVCLAASLFGWLFTLALAWHRVLLFLAALCLIKPGLITDAVGLILLAV
                     VAAAQLLARRRGATSPAS"
     gene            complement(9150..10148)
                     /locus_tag="VIG70_08160"
     CDS             complement(9150..10148)
                     /locus_tag="VIG70_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY8250564.1"
                     /translation="MFWKLAIAVGAAVAASFVPVLAKYWFWILLIGLVVAFVIEGVRI
                     VPQQSAWVIERLGRFHGTLEPGLNLIIPFLDRVAYVHSLKEVPLDVPEQVCITKDNTQ
                     LGVDGILYYQVTDPRLASYGSSNYIAAISQLAQTTLRSEIGKMELDKTFESRDEINRQ
                     IVASLDEAGRNWGIKVLRYEIKSLTPPEAILRSMQQQITAEREKRALIAKSEGERQQE
                     INVADGEKQAAVLRSEGEKQAAINKAQGDATAIRVIAEANAAAVRAVAEAIGDKGGMD
                     AANLKVAQQYVEAFAHLAKTSNTLILPANAGDVAGMVATAMTVLDKTRQGQAAAKA"
     gene            complement(10159..10584)
                     /locus_tag="VIG70_08165"
     CDS             complement(10159..10584)
                     /locus_tag="VIG70_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY8250565.1"
                     /translation="MDHAIVWAVTGLVLVIVELLTGTFYLLMLGIAAFGAAAAAYFGF
                     EFGAQASVAAVISAAGCYGVHVYKARNRAQQMAPIDAGMPASFESWLDAGARLARVRY
                     RGASWDARVEGLEALEPGATVYVLAADGNTLRVAKNRPA"
     gene            10613..11389
                     /locus_tag="VIG70_08170"
     CDS             10613..11389
                     /locus_tag="VIG70_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HEY8250566.1"
                     /translation="MLGEELVQVHQAARIVPPFSERYPGLTPDAGYRAARTLHEHRLA
                     SGWKPAGRKIGFTNRTLWERYGVYEPMWGMVYDRTLIHAQSDRASVSLAGLVQPRIEP
                     EICFRLSRRPRNAHPDEILSCIEWMAHAVEIVQCHHPDWKVTIADCTADNGLHGRLIV
                     GTPAPLNDLEKLPVVEAKLFKGERLVDRGVGANVLGSPLASLGFLVELLEKQPEAPPL
                     AAGEIVTTGVLTDAHPVAPGETWHTELSGLPLHGLRISFE"
     gene            11386..12564
                     /locus_tag="VIG70_08175"
     CDS             11386..12564
                     /locus_tag="VIG70_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY8250567.1"
                     /translation="MRPLEGITVVALEQVIAGPFATRQLAELGARVIKIERPQGGDPA
                     RAYDRTVKGLSSHFVWVNRSKESLALDVKQPAAKAVMEKLLGKADVFLHNLAPGAVER
                     LGLGSRELREKHPRLIWCGISGYGPAGPYASKKAYDLLVQCEAGLLSVTGTSESPVKA
                     GIPAADIAAGMYAFSSILAALVRRSRSGEGATIDVTMLEALGEWMGFPAYFTAYGGAA
                     PPRSGAYHATIVPYGPFRAGDGETVFLAVQNEREFAAFCDLVLKNSALKADERFASGP
                     ARSRNREAMHAEIDRVFSQLASTQVVERLETADIANARLNDMGQFWRHPQLAARGRWA
                     KVGSPVGEIDALKPPFNLSGFEPRMDAVPALGEHSRSILAELGYAEADVANLVKTSAI
                     "
     gene            complement(12557..12772)
                     /locus_tag="VIG70_08180"
     CDS             complement(12557..12772)
                     /locus_tag="VIG70_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250568.1"
                     /translation="MSETDCRSAKWYDLGEREALIYGMRPQIELYTYQCAKHGVEASE
                     KDYLAGWNVGQGERIRRAAGEGCCSPR"
     gene            complement(12832..13062)
                     /locus_tag="VIG70_08185"
     CDS             complement(12832..13062)
                     /locus_tag="VIG70_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HEY8250569.1"
                     /translation="MLVAAALLAGCAGMSDAECRSANWYDVGWRDARFKLQSQDNLYV
                     AQCERYGVKIDLAGYEKGYREGRWEYPDRTPL"
     gene            complement(13067..13300)
                     /locus_tag="VIG70_08190"
     CDS             complement(13067..13300)
                     /locus_tag="VIG70_08190"
                     /inference="COORDINATES: protein motif:HMM:NF022426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HEY8250570.1"
                     /translation="MFILLLAGCAGMDEVECRRANWYDLGFRDAIFGIMAQDDVYALQ
                     CEPVGVKVDAARYLQGFREGTYEADRRRVSAHD"
     gene            complement(13352..14470)
                     /locus_tag="VIG70_08195"
     CDS             complement(13352..14470)
                     /locus_tag="VIG70_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HEY8250571.1"
                     /translation="MQATQIRQKDGVFYFASYPAKDLLGKVRFISRFYGEGEEISPSR
                     ISEDDDIAQFISRIERTDKAFQRSLSRSKVRQLKNFYETAISQPPIPGTVLLFTSERL
                     NFRADGGGGAGSLSEPSAKYLIIDGQHRLAALRFYLHERPDDAASINVPCVIFDGRSE
                     DFATEMFVIINSTPTRINKSHLVDLYEHVSFAAPDRKFAARLVEKLYTEGDSPLRYRI
                     NRLGGRSQRDKWVLQAELFNELHRWVRGRWRSIQLAGGSSKEVPRYYAVIRDFFKAAR
                     SVFGDTVWGKENYMVTKPVTIKAMVRVCADLARDDAEPEAGRVARWEQRLGPWGEMAR
                     QFRDEGFYERFPAKGEVERVARIHRDLGRAAGIEAKKS"
     gene            complement(14544..15302)
                     /locus_tag="VIG70_08200"
     CDS             complement(14544..15302)
                     /locus_tag="VIG70_08200"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEY8250572.1"
                     /translation="MAMLREVFLVGALLVFPAQAAQCVSESGPGTTALVELYTSEGCS
                     SCPPADRWLASLGQRYAPASVVPLALHVDYWDYIGWKDPYAKREFALRQKKLTQLQRL
                     AFVYTPQVVVQGRDFRGWGTKSFDDELARINAHPARARLKLEMLGADANGLRVEASAE
                     LLAPVDDAVLYLAAYRSGLESRVMAGENRGRILTHDQVVLDWLGPFPITGRRVERRTL
                     PLLPGAGAAGSGAAAFVQSRRTGEVLQALLRSAC"
     gene            15352..16500
                     /locus_tag="VIG70_08205"
     CDS             15352..16500
                     /locus_tag="VIG70_08205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8250573.1"
                     /translation="MLPHPFFFLASLAWNFGLGMTWLAVPLYAYSQGLSNSEIGILFA
                     LPVLAQAPLNLVGGAYTDRIGGRRILLGSCIATIAAGLWFVFAKGFWMLLAGQAVFIL
                     ARAAFWPANWAMASELPGARGVQLGRLNATTNLGQIVGTTLCGFLLAAAGFQATFFVL
                     AATGAIAFVAALGTAAQPSKPPPARHVLASYLPLLREKIIAYSILCAYVSALPFSLSM
                     SFYPVLLAHYGYGEATSGVLLALRAVGSIFASLLAARFVRTGPQTLWPVGCAIAVAAA
                     VGLVPTVNHIAPIAFWLLVVGAGTAAMTLYFQITIAEASRVEERGSALALGGMGWSVS
                     HLTTPLVMGFVADRYGIVTGFHVIGAFALACALAIALMRRWAFSRRPA"
     gene            complement(16502..18676)
                     /locus_tag="VIG70_08210"
     CDS             complement(16502..18676)
                     /locus_tag="VIG70_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8250574.1"
                     /translation="MAAACLAAGLALHSLLSAAQGPRIGLVLSGGGARGVAHIGVLKV
                     LEELRVPVHCVAGTSMGAIVGGTFASGMPIAEMEKFVVEANWDDVFRDQPPRSEVASR
                     RKIDDYKTLFAPEYGVKGGLALPKGVIAGVSIEAFFRRLTDRAVAVRDFGQLPIPFRA
                     IAADIETGEAVVLDGGSVSQAMRASMAVPGALAPVLIDGRLLVDGGIANNLPIDEARK
                     LCADVVIAVNIGTPPLKRGEITSALSVAGQLVSFLGAAAVERQLKSLGARDVLIAPEL
                     GDISAGSFERVADAIRIGEEAARKMSSVLGRYSLPPGEYAALRGRQTLARKELGIVDE
                     IRFEGLERTNPGVLRSLMHSRPGEPLTEEVVSADLRRIYGRGDFESVDYRIQQEPGSR
                     VLVVRPQEKEWGPDYLRFGLGLASDFHGDNIFNVLVSYRSTWLNRLGAEWLTEAQVGS
                     NPYLSTEFYQPIEERGRYFVAPYFRVGQDSRGVFVGEDRVAEYESRETRVGLDFGAVF
                     GTLGDARLGLLSRQVDTKVDTGLPALPSLSETSAGARALLYLDQLDYAWFPRAGWRFL
                     ASAYVADEAFGSDRNYKRLEGQFTIAKSSGAHTLNLAVSGGTDLDTDLPPYETFTLGG
                     PLRLSGYRLNEFSGREMAFARLVYYNRALPLPDILGAGVYAGASLEAGQVRNRFDGLP
                     NEGTAWSGSLFLAANTFVGPAYFGLGAGESGRWSLYLLLGAP"
     gene            complement(18704..20059)
                     /locus_tag="VIG70_08215"
     CDS             complement(18704..20059)
                     /locus_tag="VIG70_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY8250575.1"
                     /translation="MTLIAKDRGPVLLVDDDKDLLQLIAMRLSATGYAVTAVASGEAA
                     LAALAVARPQVVVTDLRMQGMDGMALFDAIHRDSPSLPVVILTAHGTIPEAVTATRRG
                     VFSFLTKPFEPKVLLDTVAEAMRLSSPPASGQLEGWRIEIFTRSPAMEDLLSQARRVA
                     ASDASVCIFGASGTGKELLARAIHRASPRAEAPFVAVNCGAIPEGLLESELFGHKKGS
                     FTGAVADRRGLFQAAQGGTLFLDEIGDMPLPLQIKLLRALEERKVRPVGSHENLDVDV
                     RVIAATHRKLEERIASGEFREDLYYRLNVVKLYIPTLAERREDIPLLANEFLTRLGTR
                     YRRGPLAFSPEAMELLVSAPWPGNVRQLLNVIEQAVALSATEVIPESLVGQALNAGDT
                     SLTPLDEARRAFERDYLVRILKITGGNVTKAARLAGRNRTEFYRLLERHSLEPSVFKA
                     A"
BASE COUNT         3304 a         6845 c         6708 g         3334 t
ORIGIN      
        1 tcttccggcg agctcgccac cacgtcgaag ccgatctggt tcatgcgctc ggcgatatcc
       61 ggcgagcgca gcgccttgac catcgcgccg tgcagccgcg tgaccacgtc cttgggtgtc
      121 ttggccggcg cggccgcggc ctgccaggag gtgaccacca ggtcagcgaa acccgcctcg
      181 cccgcggaag gcacgtcggg aagctgcggg tgccgggctt tcgcggtcac cacgagcgcc
      241 ttcatccgct tgcccttgat gtagttggtc accgcgccga ggttctggaa cgagaaatcg
      301 acctgattgc ccatgatgtc ggtctggcag gctgcgccgc ccttgtaggg aacgtgcacc
      361 gcgtcggtgc ccgaggcctg cttcagcaac tcggcggtga ggtgatccga cgagcccgtg
      421 ccgctcgagc agtaggagag cttgcccgga ttcttcttca tgtggtcgac cagctctttc
      481 agcgacgcag ctgcgagcgt cggcttggtg atcagcacgt tgggcgtggt gacggcgagg
      541 gtgatcggcg cgaaatcgcg caccggctgg tagcgcaggt ccttgaacag cgccgcattg
      601 atcgagaaca cgccgatcga gccgacgagg atcgtgtggc cgtcgggctc ggccttggcg
      661 acgaattccg cggcgatcgc gccgttggcg ccagggcggt tttccaccac ccccgcctgt
      721 ccgaggagct cctgaaactt cgacgcgatc gcgcgcccga cgatgtcgga ggagcccccg
      781 gcgacgaacg ggatgatcac gcggatcggt ttcgacggga aactctgcgc tgccgcgccg
      841 ctcgcgacga ggacgagggc aaatgcgatg aaacgggtca tgggctctcc ggtggaagcg
      901 gcgcagttta ctgctaaagt tcgcgcgagg actgtcccaa ggaggagacc cccaatgcgc
      961 gccctgttcc tggttgtcgt ttcgtttttc agcgctgccg ttgcgcttca catccccggg
     1021 gcgctcgccc agccctatcc cggcaagccg atccgcatca tcgtgcccta tgccgccggc
     1081 ggcacttccg acatcctggc gcgccagatc ggaccgaagc tcaccgacgc gtggggccag
     1141 ccggtgatcg tcgagaaccg gccgggcgcc aatggcaacg tcggcgccga cttcgtcgcc
     1201 aagagcgcgc ccgacgggta caccctgctc ctcaccgacc tcggcgggct ggtgatcagc
     1261 gcgagcgtct atcccaagct gccgttcaac ccgtcgaagg acttcgcgcc ggtggtgatg
     1321 gtgtcttatt cgccgcacgt cctcgcggtg aacccggagg tgcaggtgaa gaacgtgaag
     1381 gagctcgtgg cgatggccaa ggcctatccg ggcaagctca atttcgccat ctcgggcatc
     1441 ggcggcgcgc cgcagcttgc cggcatcgag ttcgcgcagc gcatgggcat cgactggacc
     1501 tacatcccct acaagggcgg cgccgacgcg atcaccggcg tggtcggcgg ccaggcgcac
     1561 gtcctgttca acggcatgct cgcgacctgg ccgcacgtca ccggcggcag gctgaaggcg
     1621 ctggcgatct cgagcgcgca gcgcatgccg agcgcacccg acacgccgac cgtcgccgag
     1681 cagggcttgc ccggcttcga aaccggatcg ttccagggtg tggtcggctc gcgcggcacc
     1741 ccgcgcgaga cggtggcgaa gctcaacgcc gagctggtga aggtgctcaa cacggcggag
     1801 atgaaggaac gcttcgcccg gcagggtacc gacgtgcgta ccggaacgcc ggaggcgctc
     1861 ggccgatgga tggagagcga gcaggccaaa tgggcgaaag tggtcaagga ctcgggagcg
     1921 aagtttgact agcaggaaga agcccgagga gctgcgcagc caccgctggt acggggcgga
     1981 cgacctgcgc gccttcggcc accgctcgcg caccgcgcag atgggctacg accgcagcga
     2041 ctatgccggc aagccggtga tcgccatcat caacacctgg tcggacatca acacctgcca
     2101 cacgcatttc aagcagcgcg tggaggaagt gaagcgcggc gtgtggcagg cgggcggctt
     2161 cccggtcgag atgccggcga tgtcgctcgc cgagccgatg cagaagccga ccaccatgat
     2221 gtaccgcaac ttcctcgcca tggagaccga ggagctgctg cgctcctacc cggccgacgg
     2281 cgcggttctg atgggcggct gcgacaagac tacgcccgcg ctcatcatgg gcgcgatcag
     2341 catgaacctg ccgtcgatct acatgcccgc cggccccatg ctctccggcc attggcgcga
     2401 gaccacgctc ggctcgggct cggacagctg gaagtactgg gcggagctgc gcgccggcac
     2461 catcacgcgg aaggactgga acgagattga ggacagcatc gcgcgctcgc ccggaacctg
     2521 catgaccatg ggcacggcgg ccacgatgat gtcgctcgcc gagagcctcg gcttctcgct
     2581 gcccggtgcg gcctcgattc ccgctccgga ttccaaccac gccaagatgg ctacgctcac
     2641 cggcaagcgc atcgtcgaca tggtgtggga ggacctcaag ccgcgcgact tcctgacccg
     2701 ggagtcgttc gacaacgccg ccgtcaccct gatggcgatg ggcggctcga ccaacgcgct
     2761 gatccacctg gtcgccatgg ccggccgcgc cggcatccgg ttcccgctcg agcgcttcaa
     2821 cgagttctcc gcgaagacgc cgctcctcgc caacgtgcgg ccgtcgggcg acaagttcct
     2881 gatggaggac ttctactatg ccggcgggct gcgcgcgctg ctggctgagc tgaaggatct
     2941 cttgaacctg gactgcagga cggtgaacgg caagacgctc ggcgagaacc tgcagggcgc
     3001 gagcatctac aacgaagacg tgatccggaa aagaagcaat cccctgaaag cctcgggcgg
     3061 cctggtcgtg ctgcgcggca cgctcgcgcc caacggcgcg gtgatcaagg cggcggcgac
     3121 ccaaatcctg aagcacgtcg ggaaagcggt cgtgttcgac gactacaacg acatggcagc
     3181 gcgcatcgac cgcgacgacc tcgactgcga cgaggactcg gtactggtgc tgcgcaacgc
     3241 cggtcccctc ggcgggcccg ggatgcccga gtggggcatg ctgccggtgc cgaagaagct
     3301 cctgaagcag ggcgtgcgcg acatggtgcg gatctcggat gcgcgcatga gcggcacgag
     3361 ctacggctgc tgcgtgctgc acgtcgcccc cgagtcctac gttggcgggc cgctcgctct
     3421 ggcgaagagc ggcgacctga tcgagctcga cgtggagaag cgggtcctga acctgaaggt
     3481 cgccgatgcc gagctcgcga agcgcaagag cgcctggaag cccccggtac ggaaatacga
     3541 gcgcagctac ggcgcgatct tcgcgcagca cgtgaagcag gccgaccagg gctgcgactt
     3601 cgacttcctc gaaggcacgg cggcggtgcc ggagccggag atccactgat ggaaatgccg
     3661 cagaacgcct tcaagcgcgc gctgcgcgca ggcaagccgc agatcggcct gtggtcgagc
     3721 ctgtcgtcga actacaccgt ggaggtgatc gccggcgccg ggttcgactg gatcctgctc
     3781 gacagcgagc actcgccggc ggacatcgag agcctgctga cacagctgca ggccgcggcg
     3841 gcgtatccca cccatcccgt ggtgcgcgtg ccgtggaacg acatggtgaa catgaagcgc
     3901 gtgctcgaca ttggcgccca gtcgcttctc attccctacg tccagaacgc cgcggaggca
     3961 cgcagcgccg tggcgcacac gcgctacccg cccgccggcg tgcgcggcgt cgccggcacc
     4021 acgcgcgcca cccgcttcgg ccgcatcaag gactacgcgc accgggcgca cgaggagatt
     4081 tgcgtgctgg tgcaggtcga gacccagccg gcgctcgaca acatcgaggc gatctgcgaa
     4141 atagacggcg tcgacggcgt gttcatcggt cctgcggacc tgcacgcgtc catgggccat
     4201 gccggcgaag tcgcgaaccc caaggtgaaa ccgctgatcg acgacgccat ccgccgcatc
     4261 cgcaagagcg gcaaggcgcc gggcattctc acgccgaacg aggccgacgc gcgccactgg
     4321 ctcgagtgcg gggccctctt cgtcgcggtc ggcgccgacg tcggcatcct ggcgcggggc
     4381 gcggaagcgc tcgccgcgaa attcaagaaa ccatgaaacc cgagatcatc atcaccgcca
     4441 agggccacgc cggcacgatg gccacgctcc aatccgagtt cacgtgcaac cacctcttcg
     4501 aagcggcgga aaaagccccc ttttttgaaa agtgcgcgcc gcgcgtgcgc ggcctggcca
     4561 ccttcggccc gatgccggtc gacggcaagc tgatggacgc gctgcccaag ctcgagatca
     4621 tctcgaactt cggcgtcggc gtggacgcga tcaacctgga cgatgcgaag aagcgcaaga
     4681 tcatcgtcac caacacgccc gacgtgctga acgagtgcgt cgccgacacc gcgctcgcgc
     4741 tggtgatgaa cacggtgcgc aagttcccgc agtccgagcg ttacctgcgc gccggcaact
     4801 gggcctcgcg cgggccctat cccctcacca cgtcgctcgg cggcaagacg ctcggtatcc
     4861 tcggcctggg acgcatcggc gaggcgatcg ccaagcgcgc ggcggcgtgc ggcatgaaga
     4921 tccgctacca caaccgcaac cggaaggacg tgccctatcc gtacgatccg gacccggcga
     4981 cgctggcgaa gaatgcggac gtcctgatcg tcgtcacgcc cgccagcgcg gaaacgaatc
     5041 gaatcatcaa tgaaaagatc cttcaggcac tggggccgga aagctacctg gtgaacatcg
     5101 cccgcggctc ggtggtggac gagccggtgc tgctgcgcta cctgcaggag aagaagatcg
     5161 ccggcgccgg gctcgacgtg tacgccgacg aaccgcgggt gccgccggag ttcttcaccc
     5221 tggaaaacgc ggtgctgttc ccgcacgtcg ggagcgccac cgtcgaaacg cgcaaggcga
     5281 tgggcgacct gcagatcgag aacctgcggc ggcacttcgc cggcaagccg gtcgtgacgc
     5341 gggtggtcta gaagtgttag tggtcactac ggggtaaggc tttgtcgcgc ctgtagaaaa
     5401 tgacttgaca ctgtagatga gaattattat cgtttgttga tgaatcgccc tcccaaggaa
     5461 gaaccaaagc gcatctccag ccaagcgctg ctggagggcc gacgggagct ggtgatcctg
     5521 cacaacgggc gcgaataccg cctgaggctg acgcagaacg gaaagctgat cctgaccgct
     5581 taggccgtac aaccccgcga gccagcaagc ggcgcgtgtg ccgcagccag ccagcacttc
     5641 cctacccagg agctggctat gcaccgcaac gaccccgagg agagccgcgt cgagaccatt
     5701 accgcggcgc tcgtgtatct catgacccac tacgcgcgca ccggctgccc gcgcctagcg
     5761 gtgtgtgtgt cgcgccacct gcaggcgctc gcgctccatc ccgacgccgc cccggtgctg
     5821 cgcgacattt gcgctgcgct gcatggcgcc tgggccaacg ccgccgctga cgacccgcaa
     5881 atccactagg ctagaatcct cggctcaccg aggaggatgg aatgaaattt cagaaaaccc
     5941 tggtcgcggc ggccgtcggc ctgctcgccg cccccgccgc cgcccagcag gtgacgttca
     6001 tgaccggccc gcaaggcggc tcctggatcc cgctcggcgg cgcgctgaaa ggcatgtggg
     6061 agaaggcggt tcccggactg agcatcacgt cgacgcccgg cgcaggcatc gccaacgtgc
     6121 gcggcgtcga cgagggcaaa gcgcagatcg gtctcgcgaa ctcgaacacc acggtcgacg
     6181 gcctcgaagg ccgcgcgccc taccccaaga aggtcacgaa ggtctgccag ctcgcaaacc
     6241 tgtacccgca gtacttccag gtcgtcgcgc tttccgacgc gaaaatcaac agctacgccg
     6301 atctcaaggg caaggcgctg gtcacgcagc cgaagggcaa caccgccgag atgttgaccg
     6361 agatgatcct caaggtgaac ggcctgtcct accagtcgct gtcgaagacc aacttccagg
     6421 ctgcctacac cgacgcggtc tcgatgatga aggacggcca cgtgcaggtg ttcacgctgg
     6481 ggactacggc gcccgcttcg gcggtgatgg acctcgccag cgcgcgcgac gtcaagctcg
     6541 tgccggtcga cgacaagacc ttcaacgcgg tgaagaaggc gaatcccggc cacaacaagc
     6601 tgatcatcaa ggccggcacc tatccgaagc aggacaagga cgtgccggtg atcggctact
     6661 cgacgcacct ggtagtcgcc tgcgatctgc ccgagaacac cgtgtaccag atggtgaaga
     6721 ccatggcggc gaacgtggac agcatggccg cggtggtgaa atccatcgaa ggcatgaagc
     6781 cgaaggacat ggcgcaggac atcggcgtgc cgttccacaa gggcgcgagc aagttctaca
     6841 aggaagccgg cgccctgtag cagtcgcctt tggaagaaga aaagccgcag acagcgcccc
     6901 agggaggggc gctgaaggtc gcagtggcgc tgatcgccat cgcgatgtcc ctttaccaca
     6961 tgcacgcggc ggcgttcggc ccgcccgagg cgctgatctt ccgcggcacg cacctgctgt
     7021 tcgcggtgac cctcgtgttt ttgctctacc cgacgcgccc cggcggcggc ctgggttggc
     7081 gcgcgctcga tgccgtactg atgctcgccg gctgggggat cgtgctgcac atcttcttca
     7141 actaccagta ctacaccaac cgcatcatct acatcgacga gctcaccttc caggacaagt
     7201 tctgggcggt ggccgcgatc ctcatcgtgc tcgagggcac ccggcgcgtg ctcggctggg
     7261 cgctgccgct gaccgcggtc gccttcctcg cctatgccgt cagcacccag gtcacgtttc
     7321 cggtgctgtt ggagcaggtg tacctctcga ccgaagggat cttcggctcg acgctcggcg
     7381 tgtcggcgtc gtacgtcatg atgttcgtgc tgttcggcgc gttcatggaa aaaagcggca
     7441 ccgggcagct gttcatggat ttctcgatgt cgatcaccgg gcgctacgcc ggcgggccgg
     7501 gcaaggtggc cgtcgtgagc tcgagcctct tcggcacggt ttcgggaagc gccgtggcga
     7561 acgtgatggt ggacggcccg atcaccatcc cgctcatgaa gcgcaccggg ttccgcccgc
     7621 ccttcgcggc ggcggtcgag gcggtggcct ccaccggcgg gcagctcatg ccgccgatca
     7681 tgggcgcggc ggctttcgtc atggccgagt tcctcgccgt gccgtacgcc cagatcacgg
     7741 tgtgggcgat cctccccgcc ttcctctact acctcgcggt gttcgcggcc gtgcatttcg
     7801 aggccaagcg ctacaaactc gccggcgtcc ccgcgtcgga gctgccgcgc ctgggcaacg
     7861 tgatgctcac ccggggccac ctcttcatcc cgatcctgct cgtgctcacc gggctgatct
     7921 acggctactc ggcgccgctg tgcgcgctgg tcggcgcgct gtcctgcctg ccgctcgcgc
     7981 tcctcaagcg cgcgacgcgc acgggaatct cgtggcgcag cgtgctcgag gcgctcgagg
     8041 aaggtgcgaa gaacacgctc gcggtagcga tggcctgcgc cgccgccggc atcgtcatcg
     8101 cctgcgtcac gattaccggc ctgggcatcg tgttcactca ggtcgtggtc gccctcgccc
     8161 aggacacgct gatcctcgca ctgatcctga ccgcgatggc cggcatcatc ctcggcatgg
     8221 ggatgccgac cacgcccgcc tacatcatga tggcggcgct gctgattccc gcgctgatca
     8281 agctcggcat cgccggctgc ccggtgccgg tcacgcccga gtgcaaggcg gcggtgatcc
     8341 cggcggcgca catgtttgct ttctatttcg ccatcctttc ggcgatcacc ccgccggtgg
     8401 cgctggccgt attcgccgcg gctagcctcg ccaaggcaaa catgtggcaa tcgggattcg
     8461 ccgccatgcg cgccgcggcg ccggcctaca tcgtgccgtt catgttcgtc tacgagccgc
     8521 tgctgctgct gatcgtcaag gactggtcgg tcgagtggct gttcgtggtg tggtcggtca
     8581 tcacggcctc gatcggcgtg gtgtgcctcg cggcgagcct gttcgggtgg ctgttcaccc
     8641 tggcgctcgc ctggcaccgg gtgctgctgt tcctggccgc gctctgcctc atcaagccgg
     8701 gactcatcac cgacgccgtc ggcctgattc ttctcgctgt cgtcgcggcc gcacaattgc
     8761 tcgcgcgtcg ccgcggcgcg acgtcgcccg cgagctaacg gcaattccgg gaaacgcgac
     8821 aatgtgacac ggggcgcggc gatagcgcac cgtacttgcc tcgcgcaaaa tgggtcctgg
     8881 aaatgaaaga gggcacagcc gctagccgtg ccctccggga ttgcctctca agcggcgatc
     8941 aagcagtaac tgcactttgt ttggccctac cgtggacggg tcccgctttc ggatccgtct
     9001 ctcgggcttg gggctgctaa cagcgtgctg gccacctacc tgttcgatgt agagcgtgct
     9061 ctacatgggt agggatgatg cgacctttct gtcgggtttt ccatcctcct gtaggcagat
     9121 agccatagtc cggtaggact atcgctacat caggctttgg ctgccgcctg cccttggcgc
     9181 gtcttgtcga gcaccgtcat cgcggtcgcc accattcccg ccacgtcgcc tgcattggcg
     9241 ggaagaatca aggtgttcga ggtcttggcg agatgcgcaa aggcctcgac gtactgctgc
     9301 gccaccttga ggttcgccgc gtccatgccg cccttgtcgc cgatcgcctc ggccaccgcg
     9361 cgcaccgccg ccgcgttcgc ttccgcgatc actctgatgg cggtggcatc gccctgcgcc
     9421 ttgttgatcg ccgcctgctt ctcgccctcg gagcgcagca ccgccgcctg cttctcgccg
     9481 tcggcgacgt tgatctcctg ctggcgctcg ccctccgact tggcgatgag ggcgcgcttc
     9541 tcgcgctcgg cggtgatctg ctgctgcatc gagcgcagga tcgcctcggg cggggtcagg
     9601 ctcttgattt cataacgcag caccttgatg ccccagttgc gccccgcctc gtcgagcgag
     9661 gcgacgatct ggcggttgat ctcgtcgcgc gactcgaagg tcttgtcgag ctccatcttg
     9721 ccgatctcgc tgcgcaaggt ggtttgcgcg agctgggaaa tcgcggcgat gtagttcgac
     9781 gagccgtacg aggccagccg cgggtcggtg acctggtagt agaggatgcc gtcgacgccg
     9841 agctgggtgt tgtccttggt gatgcacacc tgctcgggca cgtcgagcgg cacctccttc
     9901 agcgagtgca cgtaggcaac gcggtcgagg aacggaatga tcaggttcag ccccggctcg
     9961 agcgtgccgt ggaagcggcc gagccgctcg atcacccacg cgctctgctg cggcacgatg
    10021 cgcacgccct cgatgacgaa cgccaccacc aggccgatga gcaggatcca gaaccagtac
    10081 ttcgccagga ccgggacgaa cgacgcggcg actgccgccc cgacagcgat tgcgagcttc
    10141 cagaacatgc aaccctccct aggccggacg attcttcgct acacgaagcg tattgccgtc
    10201 ggcggccagc acatagaccg tcgcccccgg ctccaaagcc tccaacccct ccacgcgcgc
    10261 atcccagctc gcgccgcggt agcgtaccct cgccagccgc gccccggcgt cgagccagct
    10321 ctcgaagctc gccggcatcc cggcgtcgat cggcgccatc tgctgggcgc ggttccttgc
    10381 cttgtagacg tgaacgccgt agcagcccgc agcgctgatc acggccgcca cgctcgcttg
    10441 cgcgccgaat tcgaatccga agtaggccgc tgccgcggct ccgaaagccg cgatgccgag
    10501 catcagcagg tagaacgtgc ccgtcaggag ctcgacgatc acgagaacca gcccggtcac
    10561 cgcccagacg atcgcatggt ccatgcgacg gctaatttac tagaatcgcc gcatgctagg
    10621 cgaagagctg gtgcaggtac accaagccgc ccggatcgtc ccacccttca gcgaacgcta
    10681 tccgggcctc actcccgacg ccggctaccg cgcggcgcgc acgttgcatg agcaccgcct
    10741 ggcgagcggc tggaagcccg ccggccgcaa gatcggcttc acgaaccgca cgttgtggga
    10801 gcgctacggc gtgtacgagc cgatgtgggg catggtctac gaccggacgt tgatccacgc
    10861 gcagagcgat cgcgcttcgg tcagcctcgc cggactggtg cagccgcgca tcgagcccga
    10921 gatctgtttc cgattgagtc gtcggccccg gaacgcccac cccgatgaaa tcctttcgtg
    10981 catcgaatgg atggcgcatg cggtggagat cgtccagtgc caccatcccg actggaaggt
    11041 cacgatcgcg gattgcaccg cggataacgg cctgcatggt cggctgattg tggggacgcc
    11101 ggcaccgctg aacgacctcg aaaagcttcc ggtggtagaa gcgaaactgt tcaagggaga
    11161 gcgtctggtc gatcgcggtg tcggcgcgaa cgtgctgggc agcccgctcg cttccctggg
    11221 gtttctcgtg gagcttctgg agaagcagcc ggaagcgccg ccgctcgctg caggcgagat
    11281 cgtgacgacc ggcgtgctca cggatgcgca tccggtggcg ccgggcgaaa cctggcatac
    11341 cgagctctcc ggcctgcctc tccatgggtt gagaatttca ttcgaatgag accgctcgaa
    11401 ggcatcaccg tggtggcgct cgagcaggtg atcgcggggc cgttcgcgac gcgccagctc
    11461 gccgagctcg gcgcgcgggt gatcaagatc gagcggccgc agggcggcga cccggcgcgc
    11521 gcgtacgacc gcacggtgaa gggactctcg tcccacttcg tgtgggtgaa ccgctcgaag
    11581 gagagccttg cgctcgacgt gaagcagccc gccgcgaaag cggtgatgga aaaattgctg
    11641 ggaaaagccg atgtgttcct gcacaacctc gcgcccggag cggtggagcg gctaggcctc
    11701 ggttcccgcg agctgaggga aaagcatccg cggctcatct ggtgcgggat ctcggggtac
    11761 gggcctgcag gcccctatgc ctcgaagaag gcctacgacc tgctggtgca gtgcgaggcc
    11821 gggctgcttt cggtcacggg gacgtcggag tcgccggtga aggccgggat cccggcggcc
    11881 gacatcgcgg ccggcatgta tgcgttttcg tcgatcctgg cggcgctggt ccgccgcagc
    11941 cgcagcggcg aaggcgcgac gatcgacgtg acgatgctgg aagcgctcgg cgaatggatg
    12001 ggctttccgg cgtacttcac cgcctatggc ggcgcggcgc cgccgcgctc gggcgcgtat
    12061 cacgccacga tcgtgcccta cgggccgttc cgggcgggcg acggcgagac cgtgttcctc
    12121 gcggtgcaga acgagcgcga gttcgccgcc ttttgcgacc tcgtgctgaa gaacagcgcg
    12181 ctgaaagccg acgagcgctt cgcgtcgggc cccgcgcgca gtcgcaaccg cgaggcgatg
    12241 catgccgaga tcgaccgggt attttctcaa ctcgcctcta cgcaggtggt ggagagactg
    12301 gaaacagcgg atatcgcgaa cgcgcggctg aacgacatgg gccagttctg gcgccatccg
    12361 cagctcgcgg cgcgggggcg ctgggccaag gtcggctcgc cggtgggcga gatcgacgcg
    12421 ctcaagccgc cgttcaacct ctccgggttc gagccgcgca tggacgccgt gccggcgctc
    12481 ggcgagcaca gccgatcgat tcttgccgag ctcggttacg ccgaagccga cgtcgccaac
    12541 ctcgtgaaaa cgagtgctat ctaggcgagc agcagccttc gccggcggcg cgccggatgc
    12601 gctcgccctg gccgacgttc cagccggcga ggtagtcctt ctccgacgcc tctacgccgt
    12661 gcttcgcgca ctggtaggta tagagctcga tctgcgggcg catgccatag atcagcgctt
    12721 cgcgctcgcc caggtcgtac cacttggcgc tgcggcagtc ggtttcgctc atgttcacgc
    12781 atccggcggc gacgaagctc gccgccaaaa tcagggttct gctccatgga gtcatagcgg
    12841 tgtccggtcg ggatattccc agcggccctc gcggtagccc ttctcgtagc ccgcgaggtc
    12901 gatcttgacg ccgtagcgct cgcactgcgc gacgtacagg ttgtcctggc tctgcagctt
    12961 gaagcgggcg tcgcgccagc cgacgtcgta ccagttggcg ctgcggcatt cggcatcgct
    13021 cattcccgca cagccggcga gcaacgcggc cgcgacgagc aatgcgctag tcgtgcgcgc
    13081 tgaccctgcg ccgatccgcc tcataggtcc cttcacggaa gccctgcaga tagcgcgcgg
    13141 cgtcgacctt gacgccgacc ggctcgcatt gcagcgcata cacgtcgtcc tgggccatga
    13201 ttccgaaaat cgcgtcgcgg aagccgaggt cgtaccagtt ggcgcggcgg cattcgacct
    13261 catccatgcc ggcgcagccc gcgagcagca gaatgaacaa cggtacgacc ctcatgggga
    13321 gccaacaagc cgcgagcggg cagtattcct gctagctctt cttggcctcg atgccggcgg
    13381 cgcggccgag gtcgcggtgg atgcgggcga cgcgctcgac ctcgcccttg gccgggaagc
    13441 gctcgtagaa gccttcgtcg cggaactgcc gcgccatctc gccccagggg ccgaggcgct
    13501 gctcccagcg cgccacgcgg ccggcttcgg gctcggcgtc gtcgcgcgcg aggtcggcgc
    13561 acacgcgcac catcgccttg atggtcacgg gcttggtgac catgtagttt tccttgcccc
    13621 agaccgtgtc gccgaacacc gatcttgcag ctttgaagaa gtcgcggatg accgcgtagt
    13681 agcgcggcac ttccttcgag ctgccgccgg cgagctggat cgagcgccag cgcccgcgca
    13741 cccagcggtg cagctcgttg aagagctcgg cctgcagcac ccacttgtcg cgctggctgc
    13801 ggccgcccag ccggttgatg cggtagcgca gcgggctgtc gccctcggtg tagagcttct
    13861 ccaccagtcg cgcggcgaac ttgcggtcgg gcgcggcgaa cgacacgtgc tcgtagaggt
    13921 cgacgaggtg gctcttgttg atgcgcgtcg gcgtcgagtt gatgatgacg aacatctcgg
    13981 tggcgaaatc ctcgctgcgg ccgtcgaaga tcacgcacgg cacgttgatc gacgcggcgt
    14041 cgtcgggccg ctcgtgcagg tagaagcgca gcgcggcgag gcggtgctgc ccgtcgatga
    14101 tgaggtattt cgccgagggc tcgctcaggg atccggcgcc gccgccgccg tcggcgcgga
    14161 agttcaggcg ctcggacgta aacagcagca ccgtgccggg aatcggcggc tgcgagatcg
    14221 cggtctcgta gaagttcttc agctggcgca ccttggagcg cgacagcgag cgctggaacg
    14281 ccttgtcggt gcgctcgatc cggctgatga actgcgcgat gtcgtcgtcc tcggagatgc
    14341 ggctcgggct gatctcctcg ccttcaccgt agaagcggct gatgaagcgc accttcccga
    14401 gcagatcctt ggccgggtag ctggcgaagt agaagacgcc gtccttttgc cggatctgcg
    14461 tcgcttgcat tggcgcttgt ctccgtggcc cggcccccgg ccgggtctgt aacaggcgcg
    14521 aatactaaag gaaaatcggg gccttagcag gcagaacgta gaagggcctg cagcacctcc
    14581 ccggtgcggc ggctctgcac gaatgccgcc gccccggagc ctgccgcacc ggcgcccggc
    14641 agaagcggca gcgtgcgccg ctccacccgc cggccggtga tggggaaggg ccccagccag
    14701 tccagcacga cctggtcgtg cgtgaggatg cggccgcggt tttctccagc catgacccgg
    14761 ctttccagcc cgcttcggta agcagcaagg tagagcacgg catcgtcgac gggcgcaagg
    14821 agctcggcgc tcgcctccac ccgcaggccg ttcgcatccg cccccaacat ctcgagcttc
    14881 aggcgtgccc gggcggggtg ggcgttgatt cgcgccagct cgtcatcgaa gcttttcgtc
    14941 ccccagccgc gaaagtcgcg gccctgcacc acgacctgcg gcgtatagac gaacgccagg
    15001 cgctggagct gggtcagctt tttctgccgc agcgcgaact ctcgcttggc gtacggatcc
    15061 ttccagccga tgtagtccca gtagtcgaca tgcagcgcaa gcggcaccac gctggctggc
    15121 gcgtagcgct ggccgaggct cgccagccag cggtccgcgg gcgggcagct cgagcacccc
    15181 tcggaggtgt agagctcgac cagcgccgtc gtccccggcc ccgattccga gacgcattgg
    15241 gctgcctggg ccgggaaaac cagaagcgcg ccgacgagaa aaacctctct gagcatggcc
    15301 attggtcgaa acggcgggcg ccggcttacg gcttcccaaa cataatggcg cgtgctgccg
    15361 caccccttct tcttcctcgc gtcgctcgcc tggaacttcg gcctggggat gacgtggctg
    15421 gcggtgcccc tgtacgccta ttcccagggg ctgtccaact ccgagatcgg gatcctgttc
    15481 gccctgccgg tgctcgccca ggcacccctc aacctggtcg gcggggccta caccgaccgc
    15541 atcggcgggc ggcgcatcct gctcggctcg tgcatcgcca ccatcgccgc cggcctgtgg
    15601 ttcgtgttcg ccaaaggctt ctggatgctg ctcgccggcc aggcggtctt catcctggcg
    15661 cgcgcggcct tctggccggc gaactgggcc atggcgagcg agcttcccgg cgcacggggc
    15721 gtgcagctcg ggcgcctcaa cgccaccacc aacctcgggc agatcgtcgg tacgaccctg
    15781 tgcgggttcc tgctcgccgc ggcaggattc caggccacct tcttcgtgct ggcggcgacc
    15841 ggcgcgatcg ctttcgtggc cgctctcggg accgcggcgc agccgtccaa gccgcccccg
    15901 gcccggcacg tgctcgccag ctacctgccg ctgctgcgcg aaaagatcat cgcctactcg
    15961 atcctttgcg cctatgtctc ggcgctgccg ttctcgctct cgatgtcctt ctatcccgtg
    16021 ctgctcgcgc attacgggta cggcgaagcg acctcgggcg tgctcctcgc gctgcgagcg
    16081 gtggggtcca tcttcgccag cctgcttgcg gcgcgcttcg tgcgcaccgg cccgcagacc
    16141 ttgtggcccg tgggctgcgc gatcgcggtc gcggcggccg tgggactggt gccgaccgtg
    16201 aaccatatcg cgccgatcgc tttctggctg ctggtggtcg gcgcaggcac ggcggcgatg
    16261 acgctgtact tccagatcac catcgccgag gcgagccggg tcgaggagcg cggctcggcg
    16321 ctggcgctgg gcggcatggg ctggagcgtg tcgcatctga ccacgccgct cgtgatgggg
    16381 tttgtcgccg accgctacgg catcgtgacc gggttccatg tgatcggcgc ctttgcgctc
    16441 gcctgcgccc tcgccatcgc cctgatgcga cgctgggcgt tttcccggcg gcccgcctag
    16501 ctcacggcgc gccgagcagc aggtacaggc tccagcgtcc gctttcgccg gcaccgaggc
    16561 cgaagtacgc agggccgacg aaggtattcg cggcgagaaa cagcgacccc gaccaggccg
    16621 tgccctcgtt gggcaggccg tcgaagcggt tgcgaacctg gccggcctcg agcgaggccc
    16681 cggcgtacac gccggctccc aggatgtcgg gcaacgggag ggcccggttg taatacacca
    16741 agcgcgcgaa tgccatttcg cggccggaga attcattgag gcggtagccc gacagccgca
    16801 gcgggccgcc gagggtgaat gtctcgtagg gaggcaggtc cgtatcgagg tcggtaccgc
    16861 cggaaaccgc gagattcagg gtgtgcgcgc cggagctttt cgcgatcgta aattggcctt
    16921 cgagccgttt gtaattgcga tcggatccga aggcttcgtc ggccacgtag gccgaggcga
    16981 ggaaacgcca gcccgcgcgc gggaaccagg catagtcgag ctggtcgaga tacagcagcg
    17041 cccgcgcccc ggcgctggtt tccgagaggc tcggcagcgc cggaagacct gtgtcgacct
    17101 tcgtatcgac ttgccgcgac agcaggccga gccgcgcgtc gcccaaggtc ccgaaaaccg
    17161 ctccgaagtc gagcccgacg cgggtttccc ggctctcgta ttccgcgacc cggtcctcgc
    17221 caacgaacac gccgcgcgaa tcctggccga ccctgaagta gggtgcaacg aaatagcggc
    17281 cgcgctcttc gatcggctgg tagaactcgg tcgaaaggta ggggttgctg ccgacctgcg
    17341 cctccgtcag ccactccgcg cccaggcgat tcagccaggt gctgcggtag ctcacgagca
    17401 cgttgaagat gttgtcgcca tggaagtcgc tcgcgagacc caggccgaag cgcaggtagt
    17461 cgggccccca ctccttctcc tgcgggcgga cgacgaggac gcggctcccc ggctcctgct
    17521 ggatgcggta gtcgacgctc tcgaaatcgc cgcgcccgta gatgcgccgc aggtctgcgc
    17581 tcacgacctc ctcggtgagc ggctcgccgg gccggctgtg catcagcgag cgcagtaccc
    17641 cggggttggt gcgctcgagc ccctcgaagc ggatttcgtc cacgatgccg agctccttgc
    17701 gcgcgagggt ctggcggccg cgcagcgccg cgtactcgcc cgggggaagg ctgtagcgcc
    17761 cgagaacgga ggacatcttc cttgctgctt cctcgccgat tcggatcgcg tcggcgacgc
    17821 gctcgaagct cccggcagag atgtcgccca gctcgggcgc gatcaggacg tcgcgcgcgc
    17881 ccaggctctt cagttgccgc tcgacggccg ccgcccccag gaagctcacg agctgaccgg
    17941 caacgctgag cgcggaagtg atttctccgc gcttgagcgg cggcgtcccg atgttgacgg
    18001 cgatcacgac gtcggcgcac agcttgcgcg cctcgtcgat cggcagattg ttggcgatgc
    18061 cgccgtcgac caggagccgg ccgtcgatca gcaccggcgc gagcgcgccg ggaaccgcca
    18121 tgcttgcgcg catggcctgc gaaacgctgc cgccgtcgag caccacggcc tcgccggttt
    18181 cgatgtccgc cgcaatcgcg cggaagggga tgggaagctg cccgaaatcc ctgaccgcca
    18241 ccgcgcggtc ggtcagccgg cggaagaacg cctcgatcga cacgccggcg atcacgccct
    18301 tcggcagcgc gagcccgccc ttgacgccgt actcgggcgc aaacagcgtc ttgtagtcgt
    18361 cgatcttgcg ccggctcgcc acctccgagc gcggcggctg gtcccggaag acgtcgtccc
    18421 agttcgcttc gaccacgaac ttctccatct ccgcgatggg cataccggag gcgaaggttc
    18481 cgccgacgat ggcgcccatg ctggtgccgg cgacgcaatg caccgggacg cgcagctcct
    18541 caaggacctt gagcacgccg atgtgcgcca ccccgcgcgc cccgccaccc gaaagcacga
    18601 ggccgatgcg gggaccctgc gccgcgctca acagcgaatg cagggccagc cctgccgcga
    18661 ggcacgctgc cgccatgcgc ggagcggaaa gcctcacgca gcgctaggcg gccttgaaga
    18721 cgctcggttc gagtgagtgg cgctcgagca gccggtagaa ctcggtgcgg ttgcgcccgg
    18781 cgaggcgcgc ggccttggtg acgttgccgc cggtgatctt gaggatccgg accaggtagt
    18841 cgcgctcgaa agcccggcgc gcttcatcga gaggtgtgag cgacgtgtcc ccggcgttca
    18901 gcgcctggcc gacgagcgat tccggaatca cctcggtcgc ggaaagcgcg accgcctgct
    18961 cgatcacgtt caggagctgg cgcacgttgc ccggccaggg agcggagacc aggagctcca
    19021 tcgcttcggg cgagaacgcc agcggaccgc gccggtagcg cgtccccagc cgcgtcagaa
    19081 actcgttcgc gagcaacggg atgtcctcgc gccgctcggc aagcgtcggg atatagagct
    19141 tcaccacgtt caggcggtag tacaggtcct cgcggaattc gcccgacgcg atgcgctctt
    19201 cgagcttgcg gtgcgtggcc gcgatcacgc gtacgtccac atcgaggttt tcgtgagagc
    19261 cgaccggacg gaccttccgc tcttccaggg ctcgcagcag ctttatctgg agcggcaaag
    19321 gcatgtcccc gatctcgtcg aggaaaagcg tgccgccctg cgcagcctgg aacaggcctc
    19381 gccggtccgc tacggcaccg gtgaaggagc ctttcttgtg gccgaagagc tcggattcca
    19441 gcagcccttc cggaatcgcc ccgcagttca ccgcgacgaa cggagcctcc gcgcgcgggc
    19501 tcgcccgatg aatggctctc gccaggagct cttttccggt gccgctcgcg ccgaaaatgc
    19561 acacgctcgc gtccgaggcc gccacgcggc gtgcctggga cagcaggtct tccatggccg
    19621 gagaccgggt gaagatctcg atgcgccagc cttccagctg cccgctcgcc ggcgggctgg
    19681 aaagccgcat cgcttcggcc acggtgtcga gcagcacttt cggctcgaac ggcttggtca
    19741 ggaagctgaa gacgccgcga cgcgtggcgg tcacggcctc gggaatggta ccgtgggcgg
    19801 tgaggatcac caccggcagc gacggcgaat cgcggtggat ggcatcgaac agcgccatcc
    19861 cgtccatgcc ctgcatgcgc aggtcggtca ccaccacctg cggacgggcg acggcaagag
    19921 cggcgagcgc agcttcgccc gacgccaccg cagtcaccgc gtagccggtg gccgaaagcc
    19981 gcatcgcgat gagctgcagc aagtccttgt cgtcgtcgac cagcagcacc gggccgcgat
    20041 ctttcgctat cagggtcatt tctgtctccg gcgcagcttt tcctcgcgct cgaggatacc
    20101 gcgttcgatt gcgcgcagcg cttccagttg ctgcttgagc gcttcctcgc gcttgtcgcg
    20161 ctccttgtcc aggcggtcgc gctccttgtc c
//
LOCUS       DATBSZ010000126         3881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1930193, whole genome shotgun sequence.
ACCESSION   DATBSZ010000126 DATBSZ010000000
VERSION     DATBSZ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1930193"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VIG70_08220"
     CDS             complement(<1..935)
                     /locus_tag="VIG70_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8250576.1"
                     /translation="MRIDRRRALAAGGAILSTLALPPLRAQGKPMRIGSTLPLTGPLA
                     SLAIIHKVTAEICVENLNKRGGLLGRPVEWVLRDDQSKPELTRTLYEQLITSDKVDLI
                     QSPYATASILAAMGVAQRYNKVLLHTSFGIPKLAKYEMQFQTAGSPFDPENVWPNHIF
                     DAAAKLPKPPKTIAIVTSKFPSVHAISVGAREILKKRGLTEVLHLEYEFGNRDYGAIA
                     SRVKEANPDFLWGGTNGIDPVLMLEAMKKIDYRPPAQVHLFPAPGPMLKLPEAQGALA
                     LTTFESHPPFTDDPAIAQFVKTYGERAKEAGIPYTA"
     gene            1000..1767
                     /locus_tag="VIG70_08225"
     CDS             1000..1767
                     /locus_tag="VIG70_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY8250577.1"
                     /translation="MQKKRTSRGIQSIDTGIRLLEVMEKADGPLALKELSARAGMDPS
                     SAHRYLASFARCGLVAQGADSRYDFGPRALRLGLAALQRSDAVQATERALPGLVAETG
                     LTALLAVWSNRGPTIVHWQRNRNFVTSLNLGSVLPVARSSIGRMLLAFLPQGMTAETL
                     AYEAKREKIDREWLERELERARKARLAYADKTVIPGLHAAASVVLGWNGEAACAVALI
                     GPDAELARANNPAALALRAMCDRVSREAGAPDLRIAA"
     gene            1781..2671
                     /locus_tag="VIG70_08230"
     CDS             1781..2671
                     /locus_tag="VIG70_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8250578.1"
                     /translation="MHLVMFMKDKQPRAGVINGEDIVDINANDRSLPPTIKGILEANG
                     LSRVKKIIRSKKHRVPRRRAKLLPPISNPGLLLSVGMNYHEHLKEMKTPVPEKPAAFT
                     KSVASIIASGQPILLPAAHPDMVDWEGEFTVVIGKAGHCIPAAKALDHVAGYTIVNDV
                     SARNWVAAIFSSSGIMGPIHAWEHNLLGKMMPTFCPMGPCIATKDEVPDPDDVKIVTR
                     LNGQVMQDANTDDLVFSVVKLIEYYSQFFRFQPGDCITTGSPSGVGFGRNPKVFMKAG
                     DTIEVEVSGIGVLSNPVKAE"
     gene            2682..3788
                     /locus_tag="VIG70_08235"
     CDS             2682..3788
                     /locus_tag="VIG70_08235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8250579.1"
                     /translation="MEFGYFTLSDNHYPNNPRSANQLVLEIREQAILADKIGMHSAWI
                     GEHHFDSLGVNSRPDLVLANIASVTKNIRLAPAVTVLPLHHPVHVAEMWATLDVLSGG
                     RVDFATGRGYDRKEYAPFGADFMASAEIFEEGIDVLLKAWRSPGKWSHRGRFYDIPEM
                     AITPRPVQKPIPFYVASFSRTSLEMAAKRGLNIIYAPFAAGMVFGGLDKAVDSYREAC
                     VKAGNKPGRAMCSYFIYIADDDKQEDYGRQTQMDYFKHCVLQAFPQKPEDAPPTMQYF
                     MKIVDILKNLKKEDLSDKSILLGSPQKIIETLKRVEKSGVEEVILYFNVGNKPHTLVK
                     EQMHRFMQEIAPHFPGKHIERMKNVGRAPHPAAA"
BASE COUNT          683 a         1289 c         1277 g          632 t
ORIGIN      
        1 gcggtatacg ggattccggc ttccttggcg cgctcgccat aggtcttcac gaactgcgcg
       61 atcgccgggt cgtcggtgaa gggcgggtgc gattcgaagg tggtgagcgc gagcgcgccc
      121 tgcgcctccg gcagcttcag catcggtccg ggcgctggga agaggtgtac ctgcgccggc
      181 gggcggtagt cgatcttctt catggcctcg agcatcagca ccgggtcgat gccgttggtg
      241 ccgccccaga ggaagtccgg attcgcttcc ttgacgcggc tggcgatggc gccgtagtcg
      301 cggttgccga actcgtactc cagatgcagc acctcggtga ggccgcgctt cttcaggatc
      361 tcgcgcgcgc cgaccgagat ggcgtgcacc gacgggaact tgctggtgac gatggcgatg
      421 gtcttcggcg gcttcggcag cttcgccgcc gcatcgaaga tgtgattcgg ccagacgttt
      481 tccgggtcga acggggagcc cgcggtctgg aattgcatct cgtacttggc gagcttcggg
      541 atgccgaacg aagtgtgcag cagcaccttg ttgtagcgct gggccacgcc catcgcggcg
      601 aggatcgaag ccgtcgcata ggggctctgg atcaggtcga ccttgtccga ggtgatgagc
      661 tgctcgtaaa gcgtgcgcgt cagctcgggc ttcgactggt cgtcgcgcag cacccactcc
      721 accgggcggc cgagcaggcc gccgcgcttg ttcaggttct cgacgcagat ctcggcggtc
      781 accttgtgga tgatcgccag cgaggcgagc ggcccggtga gcggcagcgt gctgccgatg
      841 cgcatcggtt tgccctgggc gcgcagcggc ggcagggcga gcgtggacaa aatggcgcct
      901 ccggccgcga gggcccgacg tcggtcgatt ctcatgcttg ctcctcctca ggatgtgcga
      961 gtatattggc cataggccaa cgcattgtca attaaccaat tgcagaaaaa gcgcaccagc
     1021 cgcggcatcc agtctatcga caccgggatc cggctcctcg aagtgatgga gaaggccgat
     1081 ggcccgctcg cgctgaagga gctctccgcc cgcgccggga tggatcccag cagcgcgcac
     1141 cgctacctgg cgagcttcgc gcgctgcggg ctggtggcgc agggcgcgga ctcgcgctac
     1201 gacttcggcc cgcgcgccct gcggctcggg ctggccgccc tccagcgcag cgatgccgtc
     1261 caggcgaccg agcgtgcgct gccgggcctg gtcgccgaaa ccgggctgac cgcgctgctc
     1321 gcggtctgga gcaaccgcgg cccgaccatc gtgcactggc agcgcaaccg gaatttcgtc
     1381 acttcgctca acctcggctc ggtgctgccg gtggcgcgct cgtcgatcgg ccgcatgctg
     1441 ctcgccttcc tgccgcaggg catgacggcc gagacgctcg cctacgaagc gaagcgggag
     1501 aagatcgacc gcgagtggct cgagcgcgag ctcgagcgcg cgcgcaaggc gcgcctcgcc
     1561 tatgccgaca agaccgtcat ccccggtctc catgcggccg ccagcgtggt gctgggctgg
     1621 aacggcgagg cggcctgcgc cgtcgcgctc atcgggccgg acgcggagct cgcgcgcgcg
     1681 aacaatcccg ccgcgctcgc gctgcgcgcc atgtgcgacc gggtgtcgcg cgaggccggc
     1741 gcgccggatt tgaggatcgc agcttaaaga ggagacgaag gtgcatctcg taatgttcat
     1801 gaaggacaag cagccgcgcg cgggcgtcat caatggcgag gacatcgtcg acatcaacgc
     1861 caacgaccgc tcgctgccgc cgacgatcaa gggcatcctc gaagccaacg ggctttcccg
     1921 ggtgaaaaaa atcatccggt ccaagaagca tcgcgtgccg cgccgacggg ccaagctgct
     1981 gccgccgatc tccaaccccg ggctgctgct ctccgtgggc atgaactacc acgagcacct
     2041 gaaggagatg aagacgccgg tgccggagaa gcccgccgcg ttcaccaaga gcgtcgccag
     2101 catcatcgct tccggacagc caatcctgct gcctgccgcc catccggaca tggtcgactg
     2161 ggaaggcgag ttcaccgtgg tgatcggcaa ggccggccat tgcatcccgg cggcgaaggc
     2221 gctcgatcac gtcgccggct acaccatcgt caacgacgtc tcggcgcgca actgggtggc
     2281 ggcgatcttc tcctcgagcg gcatcatggg cccgatccac gcctgggagc acaacctgct
     2341 cggcaagatg atgccgacct tctgcccgat gggtccttgc atcgcgacca aggacgaagt
     2401 gcccgatccc gacgacgtga agatcgtgac gcggctcaac ggccaggtga tgcaggacgc
     2461 caacaccgac gacctggtgt tcagcgtggt gaagctaatc gagtactact cgcagttctt
     2521 ccgcttccag cccggcgact gcatcaccac cggctcgccc tccggcgtcg gcttcgggcg
     2581 caatcccaag gtgttcatga aggccggcga cacgatcgaa gtcgaggtga gcggtatcgg
     2641 cgtgctctcg aacccggtga aggctgagta aggagcaacc tatggaattc ggctacttca
     2701 ccctcagcga caaccactac ccgaacaatc cgcgctcggc caaccagctc gtgctcgaga
     2761 tccgcgagca ggcgatcctc gccgacaaga tcggcatgca ctcggcgtgg atcggcgagc
     2821 accacttcga ttcgctgggc gtcaactcgc gcccagactt ggttttggcg aatattgctt
     2881 ccgttaccaa gaacatacgg ctcgccccag ccgttacggt cttgccgctg caccacccgg
     2941 tgcacgtggc ggagatgtgg gcgacgctcg acgtgctctc gggcgggcgc gtcgacttcg
     3001 ccaccggccg cggctacgac cgcaaggagt acgccccgtt cggcgccgac ttcatggcct
     3061 ccgccgagat cttcgaggaa ggcatcgacg tgctgctgaa ggcgtggcgc tcgccgggca
     3121 agtggtcgca ccgtggccgc ttctacgaca tcccggagat ggcgatcacg ccgcgcccgg
     3181 tgcagaagcc gatccccttc tacgtcgcct cgttctccag gaccagcctc gagatggcgg
     3241 cgaagcgagg cctgaacatc atctacgcgc cgttcgccgc gggcatggtg ttcggcggcc
     3301 tcgacaaggc cgtcgacagc taccgcgagg cctgcgtcaa ggcgggcaac aagccggggc
     3361 gcgccatgtg cagctacttc atctacatcg ccgacgacga caagcaggag gactacggcc
     3421 ggcagacgca gatggactac ttcaagcact gcgtgctgca ggccttcccg cagaagcccg
     3481 aggacgcgcc gcccacgatg cagtacttca tgaagatcgt cgacatcctg aagaacctga
     3541 agaaggagga tctctccgac aagtcgatcc tgctcggctc gccgcagaag atcatcgaga
     3601 cgctgaagcg cgtcgaaaag tcgggcgtcg aggaggtgat cctctacttc aacgtaggca
     3661 acaagccgca caccctggtg aaggagcaga tgcaccgctt catgcaggag atcgcgccgc
     3721 acttccccgg caagcacatc gagcgcatga agaatgtggg aagagctcct cacccggccg
     3781 cggcctgagc acgacgcgcg ggatttccgc gccgcgctcg gtgccttccc gaccggcgtc
     3841 tgcctcgtca cgaccgccgg gccggacggc gagcgccacg g
//
LOCUS       DATBSZ010000127         7284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1998457, whole genome shotgun sequence.
ACCESSION   DATBSZ010000127 DATBSZ010000000
VERSION     DATBSZ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998457"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VIG70_08240"
     CDS             complement(<1..825)
                     /locus_tag="VIG70_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8250580.1"
                     /translation="MLHRRVAFVVTAALVFAGSAIAQTKQPPLRTGVDGTFAPHAMPK
                     LGGGIEGFNIDLFTEVAKRLKREITIDSVSFSTLIPGMLSGRYDFIAAPTTVTKERAE
                     QMLFTAGYLWTAYQFGIKKGSKPIKGWADLKGKVVSVNKGTPYETLSKKMGEQHGFTV
                     QAYDTQPDATQAVLSGRAYATLGGNTTIVYAASKNPQFVADLELTDTRAHWGAPFPKQ
                     NVALRNEVQDVLNCMKKDGTIARMSEKWFGRKPGPDALERVITPGYGVPGMPGYDPT"
     gene            complement(891..2033)
                     /locus_tag="VIG70_08245"
     CDS             complement(891..2033)
                     /locus_tag="VIG70_08245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8250581.1"
                     /translation="MQIVLFHLMPYADLDLEAGRKNGTVWVKLPNKHFDPEKGHKLYN
                     RYLDELEYGEELGFDAVSVNEHHQTAYGLMPSPVVTASALARKTKRVKIAILGSALPL
                     RSHPLTVAEEHAMIDVISGGRLISGFVRGIGAEYHTFGVNPTFSHDRFHEAHDLIVRA
                     WTEPGPFAFQGKHYNVQYVNLWPRPYQKPHPPIWIPSQGSKETIDWASHPDRRYTYLQ
                     TFSPVKVVGRYLKMYRDTCKGYGYEAKDSQLGWAVPVYVSGSDESARREAKEHFEAFR
                     NVFVRMPLEMLLPPGYTSRESLKNVMKAKAQMFGDVTLEQALELGLFVCGSPATVKKA
                     FQDYWNEMRFGNLLVMCQFGTLPADLTRKNLELFAREVMPALKALS"
     gene            complement(2024..2776)
                     /locus_tag="VIG70_08250"
     CDS             complement(2024..2776)
                     /locus_tag="VIG70_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8250582.1"
                     /translation="MKFSDTFVEVDGCKTHLRRGGAGDPLLFLHGASGAPVVMPFMEK
                     LAQRFDVLVPEHPGWGRSDEPEWLENIHDVAYFYLDFLKHLDLEKAVVVGSSMGGWIA
                     MEMAVRDTSRIKSLVLVSPAGIAAPGVLPADIFLLSPEELVKSLFFDEKLAQARLAQP
                     EDVDISLKNRHTTARLAWEPRLHDPHLGKWLHRIDVPVKIVWGRQDRILPVGFVDEYK
                     RLLPRADIHILEKCGHLPHAEKADEFVEIVCR"
     gene            complement(2773..3681)
                     /locus_tag="VIG70_08255"
     CDS             complement(2773..3681)
                     /locus_tag="VIG70_08255"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY8250583.1"
                     /translation="MSLLQTRLRNGQFVVTAEITPPVSTDPAEFLRRALPLKGLVSAI
                     NVTDGAGAKVHLSSLAAAHFLVSSGLEPIFQMTCRDRNRLALQSDLLGAASLGIHNIL
                     VLAGDDPKLGDQPDAKPVYDVDSRGLIAMARSMREQRKLPSGTAIDGKVELFIGAADV
                     PIDPPPDWSPKALMAKAQAGADFVQTQFCMDIGVVRRYSARLRELGFKLPLLIGIAPI
                     PSARSARWMREKLFGTTIPEAIVQRLEKAADPRAEGRKICVELLRELAEVPGVAGAHV
                     MAPMNFAEIPAAIQESGVLKKQRAAA"
     gene            3680..4579
                     /locus_tag="VIG70_08260"
     CDS             3680..4579
                     /locus_tag="VIG70_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY8250584.1"
                     /translation="MPVDLIIGIRPTMPELPDVTAYIEGLEKRIRGARLGSLRLLNPF
                     LLRTAVPPIGEASGKKVLGLSRIGKRIVIALEGELFLVLHLMIAGRLHWEGKKTRKTL
                     ALFEFDSGTLTLTEAGTKRRASLHLVQGEAALRAMDPGGIEVLTASPGEFRDRLVLEN
                     HTLKRALTDPRLFSGIGNAYSDEILHRAKLSPIALTKDLSDEQVERLYRSTRETLQDW
                     TQRLRTEGFPEKVTALREGMAVHGRYGKPCPVCGAPVQRIVYAENETNYCARCQTGGR
                     ILADRALSRLLKKSWPRSIDELP"
     gene            complement(4599..4991)
                     /locus_tag="VIG70_08265"
     CDS             complement(4599..4991)
                     /locus_tag="VIG70_08265"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY8250585.1"
                     /translation="MTQPQEFLDLYKAGFKTAADLMKASLQNAERLQNQQLVAIRTAL
                     DQQSKSISELGQVRSLDELLSLQTKMAGAQIERAVGFWSELYQTQMAQAQTWFNEMNG
                     AQGSVASAAAATIRQASPRNQEQHRKSA"
     gene            5127..5333
                     /locus_tag="VIG70_08270"
     CDS             5127..5333
                     /locus_tag="VIG70_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250586.1"
                     /translation="MENIVRLAEYRQRRQAAKQAAADLEAQYFCTRCDSGEFKLAAAG
                     MVHCAHCGALMRNLLVNSSSQEAG"
     gene            complement(5305..6051)
                     /locus_tag="VIG70_08275"
     CDS             complement(5305..6051)
                     /locus_tag="VIG70_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEY8250587.1"
                     /translation="MIYFVLGAVVIAVILFALSIKILREYERAVVFFLGRFQRVFGPG
                     LIVIIPGVQQAVRVDLRTVVLDVPPQDVITRDNVSVKVNAVVYFRVVDPRNAIIQVAN
                     FLEATSQLAQTTLRSVLGKHELDALLAEREKLNVDIQTVLDSQTDAWGIKVSNVEIKH
                     VDLNESMVRAIARQAEAERERRAKVIHAEGELQASEKLLQAANMLSQRTEAMQLRYLQ
                     TLTQIAGDKASTIVFPLPMDLLSPLLGKKS"
     gene            complement(6048..>7284)
                     /locus_tag="VIG70_08280"
     CDS             complement(6048..>7284)
                     /locus_tag="VIG70_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEY8250588.1"
                     /translation="SAQSSQPQVLVVPLEGAIGPASADFVGRAIKRAAKDKAQLVVVR
                     MDTPGGLDTSMRELIKDILASPVPVATFVAPSGARAASAGTFILYASHFAAMAPGTNV
                     GAASPVAIGAGSPQKDEKKDKSEDTMMRKVTNDAVAYIRGLAEMRGRNANWAERAVRE
                     GVSLSAEQALKLKVIDHMALDVPDLLKKLAGNKDLRLADAPVVEVQVDWRTKVLSVIT
                     NPSIAYIMILVGIYALIFEFMNPGMVLPGVVGAICLLLALYAFHLLPVNYAGLALIVL
                     GIAFMVAEAFLPAFGSLGIGGLIAFVIGSVILFDETALPGFEIPYALIGGVAAASAAF
                     VFFVVGVAISNRRRPVVSGREYLIGAAAEALEDFDAEGWARVQGETWRVRSNAPVRRG
                     ERLKVRSIEGLVLNVEADR"
BASE COUNT         1202 a         2381 c         2396 g         1305 t
ORIGIN      
        1 ggtcggatcg tagccgggca tgccgggcac gccgtagccc ggcgtgatca cgcgctcgag
       61 cgcgtcgggt cccggcttcc tgccgaacca tttctccgac atcctggcga tggtgccgtc
      121 cttcttcatg cagttgagca cgtcctgcac ctcgttgcgc agcgccacgt tctgcttggg
      181 aaacggcgct ccccagtgcg cgcgcgtgtc ggtaagctcg aggtcggcga cgaattgcgg
      241 gttcttgctg gcggcgtaga cgatggtggt gttgccgccg agcgtcgcgt aggcgcgccc
      301 ggagagcacc gcctgcgtcg cgtccggctg ggtgtcgtag gcctggacgg tgaagccatg
      361 ctgctcgccc atcttcttgc tcagggtctc gtagggcgtg cccttgttga ccgagaccac
      421 cttgcccttg aggtcggccc agcccttgat cggcttggag cccttcttga tgccgaactg
      481 gtacgcggtc cacaggtagc cggcggtgaa cagcatctgc tcggcgcgct ccttggtcac
      541 cgtggtcggc gccgcgatga aatcgtagcg gcccgagagc atgcccggga tcagggtcga
      601 gaagctcacg ctgtcgatgg tgatctcgcg cttcaggcgc ttggcgacct cggtaaagag
      661 gtcgatgttg aagccctcga tgccgccgcc gagcttcggc atcgcgtgcg gcgcaaaggt
      721 gccgtcgacc ccggtgcgaa gcggcggctg cttggtctgg gcgattgcgg atcccgcgaa
      781 gacgagcgcg gcggtcacga cgaaggcaac gcggcgatgc agcatcacaa aacctccccc
      841 ggaaattggt aaggcgattg tatgacctgc cgacggcatg cgcatcctcg ttaggacaag
      901 gccttgaggg ccggcatcac ctcgcgcgcg aacagctcga ggttcttgcg cgtaaggtcg
      961 gcgggcagcg tgccgaactg gcacatcacc agcaggttgc cgaagcgcat ttcgttccag
     1021 tagtcctgga atgctttctt cacggtcgcg gggctgccac agacgaataa gccgagctcg
     1081 agcgcctgct ccagggtgac gtcgccgaac atctgcgcct tcgccttcat cacgttcttc
     1141 agcgactcgc gcgaggtgta gcccggcggc agcagcatct caagcggcat ccgcacgaac
     1201 acgttgcgga acgcttcaaa atgttccttt gcttccctcc gggcgctttc gtccgacccg
     1261 ctgacataaa caggaacggc ccaacccagt tggctgtcct tggcttcgta gccgtacccc
     1321 ttgcaggtgt cgcggtacat cttgagatag cggccgacca ccttcaccgg gctgaaggtt
     1381 tgcaggtacg tgtagcgccg gtcggggtgc gacgcccagt cgatggtctc cttgctgccc
     1441 tgagacggga tccagatcgg cgggtgcggc ttctggtacg ggcgcggcca gagattcacg
     1501 tattgcacgt tgtagtgctt gccctggaac gcgaacggtc cgggctcggt ccaggcgcgc
     1561 acgatgagat cgtgggcttc gtggaagcgg tcgtgggaga aggtcgggtt cacgccgaag
     1621 gtgtggtact cggcgccgat gccgcgcacg aagccgctga ttaggcgccc gccgctgatc
     1681 acgtcgatca tcgcgtgttc ttcggccacg gtgagcgggt gcgagcgcag cggtagtgcg
     1741 ctccccagga ttgcgatctt tacccgtttc gtttttctgg caagagcaga agcggtgacg
     1801 acgggcgaag gcatcaggcc gtatgccgtt tgatgatgct cgttcaccga aacggcgtcg
     1861 aagccgagct cctcgccgta ctcgagctcg tccaggtacc ggttgtagag cttgtggccc
     1921 ttctcgggat cgaagtgctt gttgggcagc ttcacccaca ccgtgccgtt cttgcgcccg
     1981 gcctcgaggt cgaggtcggc gtacggcatc aggtgaaaca gcactatctg catacgatct
     2041 cgacgaactc gtcggctttt tcggcgtgcg gcaggtggcc gcatttttcc aggatgtgaa
     2101 tgtcagcgcg gggcagcaag cgcttgtatt catccacgaa gccgacgggc aggatgcggt
     2161 cctggcggcc ccagacgatc ttcaccggca cgtcgatgcg gtgcagccac ttcccgaggt
     2221 gcggatcgtg caggcgcggc tcccaggcga ggcgcgcggt ggtgtgacgg ttcttgagcg
     2281 agatatcgac gtcttccggc tgcgcgagcc gcgcctgcgc gagtttttca tcaaaaaata
     2341 gactcttgac gagctcttcg ggcgacaaca ggaagatatc cgcgggcagg acgccgggcg
     2401 cggcaatgcc ggccgggctg accaagacca gcgacttgat gcgcgaggtg tcgcgcaccg
     2461 ccatttccat ggcgatccac ccgcccatgg agctacccac gacaacggcc ttttccagat
     2521 cgaggtgttt caggaaatcc aggtagaagt aagcgacgtc atggatgttc tcgagccact
     2581 ccggctcgtc cgagcgcccc cagcccgggt gctcgggcac gagcacgtcg aagcgctggg
     2641 cgagcttctc catgaagggc atgaccaccg gcgcgccgct tgcgccgtgc aggaagagaa
     2701 gcggatcacc cgcgccgccg cgccgcaggt gcgtcttgca gccgtcgacc tcgacgaagg
     2761 tgtcggagaa cttcacgcgg ccgcgcgctg cttcttcagc acgcccgatt cctggatcgc
     2821 cgccgggatc tcggcgaagt tcatcggcgc catcacgtgc gcgccggcga cgccgggaac
     2881 ctcggcgagc tcgcgcagca gctcgacgca gatcttcctg ccctcggccc gcgggtccgc
     2941 ggccttttcg agccgctgca cgatcgcctc gggaatggtg gtgccgaaga gcttctcccg
     3001 catccagcgc gccgagcgcg ccgaggggat cggggcgatc ccgatcaaga gcggcagctt
     3061 gaatccgagc tcacgcaagc gcgctgaata acgccgcacc acgccgatgt ccatgcagaa
     3121 ctgcgtctgc acgaaatccg cacccgcctg cgcctttgcc atcagcgcct tggggctcca
     3181 gtcgggcggc ggatcgatcg gcacgtcggc cgcgccgatg aagagctcga cctttccgtc
     3241 gatcgcggtg cccgagggca gcttgcgctg ctcgcgcatg ctgcgcgcca tcgcgatcag
     3301 gccgcggctg tcgacgtcgt acaccggctt ggcgtcgggc tgatcgccca gcttcggatc
     3361 gtcgccggca aggaccagga tgttgtggat gcccagcgac gccgcgccca gcaggtcgct
     3421 ctgcagcgcg aggcggttgc ggtcgcggca ggtcatctgg aagatcggct cgaggccgct
     3481 acttacgagg aagtgcgcgg ccgccaggct cgataggtgc accttggcgc cggcgccgtc
     3541 ggtgacgttg atcgcgctca ccaggccctt gagcggcagc gcgcggcgca ggaattcggc
     3601 ggggtcggtg gagaccggcg gcgtgatctc cgccgtgacg acgaactgcc cgttgcgtaa
     3661 ccgcgtctgc agtagggaca tgccggttga tcttatcatc gggattcgtc ccaccatgcc
     3721 cgagcttccc gacgttaccg cttacataga gggcctcgag aaacggatac gcggcgcgcg
     3781 cctcggaagt ctccgcctgc tgaacccgtt cctcctgcgc accgccgtgc ccccgatcgg
     3841 cgaggccagc ggaaagaaag tgctgggact ctcccgcatc ggcaagcgca tcgtgatcgc
     3901 gctggaaggc gagctcttcc tggtgcttca cctgatgatc gccgggcgac tgcactggga
     3961 aggaaagaag acccgcaaga cgctcgcgct gttcgagttc gacagcggga cgctgactct
     4021 gaccgaggcg ggcaccaagc gccgggcctc actgcacctc gttcagggcg aggcggcact
     4081 tcgggcgatg gatccagggg gcatcgaagt cttgaccgcc tctcccgggg aattccggga
     4141 caggctggtt cttgagaatc acaccctcaa gcgggcgctg accgacccgc gccttttctc
     4201 ggggatcggg aatgcctact ccgacgagat cctgcaccgc gcgaagcttt cgcccatcgc
     4261 gctgaccaag gatctttccg acgagcaggt cgagcgcctg taccggtcca cgagggaaac
     4321 ccttcaggac tggacgcagc ggctgcgcac cgagggcttc cccgagaagg tcaccgcgct
     4381 ccgcgaaggc atggcggtgc acggccgcta cggcaagccg tgcccggtct gcggcgcgcc
     4441 ggtgcagcgc atcgtctacg ccgagaacga gaccaactac tgcgcccgct gccagaccgg
     4501 cggccggatc ctcgccgacc gcgcgctgtc gcgcctgctc aagaagagct ggccgaggtc
     4561 gatcgacgag ctgccctagt tagggctccg tccccgaatt acgcggactt gcggtgctgc
     4621 tcctggttgc ggggcgaagc ctggcggatg gtggcggcgg ccgccgaggc gaccgagccc
     4681 tgtgcgccgt tcatctcgtt gaaccaggtc tgggcctgcg ccatctgggt ctggtagagc
     4741 tcgctccaga agccgaccgc gcgctcgatc tgggcgccgg ccatcttggt ctgcagcgag
     4801 agcagttcgt ccaggctccg gacctggccg agctcgctga tcgatttgga ctgctggtcg
     4861 agcgcggtgc ggatcgccac gagctgctgg ttctgcagcc gctcggcgtt ctgcagggag
     4921 gctttcatca ggtcggcggc ggtcttgaag cccgccttgt acaggtccag aaactcttgc
     4981 ggttgggtca tcgtctctcc ttgctaggtt gagcgttgtc cctcccctgc tacagcgtgt
     5041 cgcttttttc cggcttcaga tgagtgtacc cctcttcgga acgcgggcgg ataaaaagaa
     5101 tcaaattgcg cagtggttga tcattgatgg aaaacatcgt ccgactcgcc gagtaccgcc
     5161 agcgccgcca agccgctaag caagccgcgg ccgacctgga ggcgcagtat ttctgcacgc
     5221 gctgcgactc cggcgagttc aagctggccg ccgcgggcat ggtccattgc gctcattgcg
     5281 gcgcgctgat gcgtaacctg ctggtcaact cttcttccca agaagcgggc tgagcaggtc
     5341 catcggcagc ggaaacacga tcgtcgaggc cttgtcgccg gcgatctggg tgagggtctg
     5401 caggtagcgc agctgcatcg cctcggtgcg ctgcgacagc atgttcgccg cctgcagcag
     5461 cttctcggag gcctgcagct ccccttcggc gtggatcacc ttggcgcggc gctcgcgctc
     5521 ggcctcggcc tgccgggcga tggcgcgcac catcgactcg ttcaggtcga cgtgcttgat
     5581 ctcgacgttc gacaccttga tgccccaggc gtcggtctgg ctgtcgagca cggtctggat
     5641 gtcgacgttc agcttctcgc gctcggcgag gagcgcgtcg agctcgtgct tgccgagcac
     5701 cgagcgcagc gtggtctggg cgagctggct ggtcgcctcg aggaagttgg ccacctggat
     5761 gatcgcgttc ctcgggtcga ccacccggaa gtagaccacc gcgttcacct tcaccgacac
     5821 gttgtcgcgc gtgatcacgt cctgcggcgg cacgtcgagc accaccgtgc gcaggtcgac
     5881 gcgcaccgcc tgctggaccc ccgggatgat cacgatcagc cccggaccga acacgcgctg
     5941 gaagcgcccg aggaagaaca ccaccgcgcg ctcgtactcg cgcaggatct tgatcgacag
     6001 cgcgaacagg atgacggcaa tgacgactgc gcccagcacg aaatagatca ccggtcggcc
     6061 tccacgttga gtaccagccc ttcgatggac ctgactttca gccgctcccc gcgccgcacc
     6121 ggcgcgttgc tgcgcacgcg ccaggtctcg ccctgcacgc gcgcccagcc ctcggcgtcg
     6181 aaatcctcca gcgcctcggc cgcggcgccg atcaggtact cgcggccgct gaccacggga
     6241 cgcctgcggt tacttattgc tacgcccaca acaaaaaaga caaacgcagc gctcgcggca
     6301 gcgaccccgc cgatcagcgc gtagggaatc tcgaagcccg gcagcgccgt ttcgtcgaac
     6361 aggatcaccg agccgatgac gaaagcgatc agccccccga tgccgagcga gccgaacgcg
     6421 ggcaggaacg cctccgccac catgaaggcg atgccgagaa cgatcagcgc gaggcctgca
     6481 tagttcaccg gcaggaggtg gaaagcgtag agcgccagca gcaggcagat cgcgcccacc
     6541 accccgggca ggaccatgcc cgggttcatg aactcgaaga tcagggcgta aatgccgacc
     6601 aggatcatga tgtaggcgat gctcgggttg gtgatcacgg acagcacctt ggtgcgccag
     6661 tcgacctgca cctccaccac cggcgcgtcg gcgaggcgca agtctttatt ccccgcgagc
     6721 tttttcagca ggtcaggaac gtcgagggcc atgtggtcga tcaccttgag cttgagcgcc
     6781 tgctcggccg acaggctcac gccctcgcgc accgcgcgct cggcccagtt ggcattgcgc
     6841 ccgcgcatct cggcgagccc ccggatgtag gccacggcgt cgttcgtgac cttacgcatc
     6901 atggtgtctt cggatttgtc tttcttttca tctttctggg gtgaaccagc gccgatcgcg
     6961 accggcgatg cggccccgac gttggttccc ggcgccatcg ccgcgaaatg gctggcatac
     7021 aggatgaagg tgccggcgct cgcggcgcga gccccgctcg gcgcgacgaa agtggccacc
     7081 gggactggcg aggcgaggat gtccttgatg agctcgcgca tcgacgtgtc gaggccgccg
     7141 ggcgtatcca tgcgaaccac gacgagctgt gccttgtctt tcgccgcgcg cttgatcgcg
     7201 cggccgacga aatccgcgct cgccggaccg atcgcgcctt cgagcggcac gacgaggacc
     7261 tgcggctgcg aactttgcgc cgac
//
LOCUS       DATBSZ010000128         4236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_564917, whole genome shotgun sequence.
ACCESSION   DATBSZ010000128 DATBSZ010000000
VERSION     DATBSZ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564917"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2237)
                     /locus_tag="VIG70_08285"
     CDS             complement(<1..2237)
                     /locus_tag="VIG70_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250589.1"
                     /translation="MNRTTLVAIAATSAALLMTELALTRIFSVTMFYHFAFLAISIAL
                     FGLSASGVFVYLTRRRLAARNTDGLLAIAAIAHMTATVLALACLVRIPVTLTYSTENL
                     WRMLAIYLLAAAPFFTGGTVLSLAFARLTARINALYASDLLGAALGCLALVPLMNLLG
                     APGVVLSAATLAAVAAVCFAPARWKRWAARAAFVVLGVPFTMQLAGLGPFEVDAIKGR
                     ERESRLLFSKWNSFSRIGVYDYPHQDWSLSPKFKGQPGPSLFMDIDASASTPILKGSA
                     DTSYLRHELTAIGYHLVERPGGFTSLVIGPGGGRDVLSALSFGAKHVEGIEINPIIVR
                     DVMLDRFRAYSGNLYSDPRVSVHVEDGRSYVRRSDKRYDLIQASLVDTWAATSAGAYT
                     LTENSLYTTEAFGEYLDHLTNDGLLSVTRWVVDGLRLVSLAQAACERRGLDPARHIAI
                     VQHKTVMTFILKRSPFSPEEVRRLQRVSDDLGFTLLYAPGVPAPDVKEPEEMLRAGTS
                     LADYRGLILAADRSRFLDAYRHDVSATTDDRPFFFHTTRLRDQVRVAFGRHMLFGSGL
                     SALLTLFMISAALVLLFIVAPLLIAGERPRRGWGGWLAYFAALGAGFMLLEVAVLQRF
                     VLLLGHPVYSLTVTLFSLLLGTGIGSLLSRRVPDDDVRRVTLYALGAAALVGLLAALV
                     LERLVDFAIPWPLPARIAAAAALLVPVGVLLGVGLPGGMRLLNRARPEIVAWGWGMNG
                     AFSVIG"
     gene            complement(2234..3367)
                     /gene="rffA"
                     /locus_tag="VIG70_08290"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(2234..3367)
                     /gene="rffA"
                     /locus_tag="VIG70_08290"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HEY8250590.1"
                     /translation="MRPVKVPFNRPHMTGRELAYIEQAHRNHRLAGDGPFTERCSRWL
                     EKQTGARRALLTHSCTAALDMAAILAGIGPGDEVIMPSYTFVSTANAFVLRGGVPVFV
                     DVRADTLNIDETLIEAAVTPRTRAVVPVHYAGVGCEMEPILELAQRRRLLVIEDAAQG
                     LMTSHRGRSLGAIGQLGALSFHETKNVISGEGGALLVNDASFIGRAEIVREKGTNRSS
                     FFRGEVDKYTWVDIGSSYLPGEIIAAFLWAQLEQAEAITARRIELWNRYHERLEACER
                     AGRIRRPVIPAGCRHNAHLYYVLLPSLEHRNRLIERLRAREIRSVFHYVPLHSSPAGR
                     RYGRTASAMTNTDRAGECLLRLPLWLGMDAGVADAIAQEIDAL"
     gene            3385..>4236
                     /locus_tag="VIG70_08295"
     CDS             3385..>4236
                     /locus_tag="VIG70_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250591.1"
                     /translation="MHYCFITGFLRSGTTLVEKLVHALPGACIGPQPFPFLYHDAKRA
                     FLRARGAGEERYPLGSLFREERYRPEEFHAFLRSHCLTPEATAASFAAMRGYSGWKLP
                     ALAEHAGQIGEGSFAEVYRKLCDRLPVILGAEASRLLGAKEVFCEEFIPYFLAEGVSV
                     VLVLRDVRDVLTSLKFGSGAAYANREMSVLHIVRQWRKSVAFALEFSGRPGFEAVRYE
                     ELVARPRAWLDALAGRWRIPAAAPGAIESLRDQEGREWESNSSFAPARGVSRDSVGRY
                     AEHLPAAW"
BASE COUNT          691 a         1408 c         1550 g          587 t
ORIGIN      
        1 ccgatcaccg agaacgcgcc gttcatgccc cagccccagg ccacgatctc gggccgcgcg
       61 cggttgagca agcgcatgcc gcccggcagg ccgacaccga gcagcacgcc gaccgggacc
      121 agcagcgcgg cggcggcggc gatgcgggcg gggagcggcc acgggatggc gaaatcgacc
      181 aggcgctcga gcaccagcgc ggcgagcaag ccgacaagcg ccgcggcacc caacgcatat
      241 agggtgacgc gccgcacgtc atcgtcgggc acgcggcggc tgagcaggct gccgatgccg
      301 gtgccgagaa ggagcgaaaa cagcgtaacg gtcagggaat agaccgggtg accgagcagc
      361 agcacgaagc gctgcagcac cgcgacctcg agcaacatga atcctgcgcc gagcgcggcg
      421 aagtacgcga gccagccccc ccagccgcgg cgcgggcgct cgcccgcgat gaggagcggg
      481 gcaacgatga acagcagcac cagcgccgcg gagatcatga acagggtgag cagcgccgac
      541 agcccgctgc caaacagcat gtgccgcccg aacgcgaccc ggacctggtc gcgcagccgc
      601 gtggtgtgga agaagaacgg ccggtcgtcg gtcgtcgcgc tgacgtcgtg gcggtaggcg
      661 tcgaggaagc ggctgcggtc ggcagcgagg atcagcccgc ggtagtcggc gaggctggtg
      721 ccggcgcgga gcatttcctc cggttccttc acgtccggcg cgggcacgcc gggggcgtag
      781 agcagggtga agccgagatc gtccgacacg cgctgcagcc ggcgcacctc ctccggcgag
      841 aacggcgagc gcttgagaat gaaggtcatc accgtcttgt gctggacgat cgcaatgtgc
      901 cgggccgggt ccaggccgcg gcgctcgcag gccgcctggg cgagcgacac caggcgcagc
      961 ccgtcgacca cccagcgcgt gaccgagagc agtccgtcat tcgtgagatg gtcgagatat
     1021 tcgccgaacg cctccgtcgt ataaagcgag ttctcggtca gcgtgtacgc gcccgccgag
     1081 gtggcggccc aggtatcgac cagcgatgcc tggatcaggt cgtagcgctt gtcgctgcgg
     1141 cgcacgtagc tgcgcccgtc ctcgacgtgg accgagaccc gggggtcgct gtagaggttg
     1201 ccggaatacg cgcggaagcg gtcgagcatg acgtcgcgca cgatgatcgg gttgatctcg
     1261 atgccctcga cgtgctttgc gccgaatgac agggcggaca acacgtcgcg cccgccgcca
     1321 gggccgatca ccaagctggt gaatccgccc ggccgctcga ccaggtggta cccgatcgcg
     1381 gtcagctcat ggcgcaggta gctggtgtcc gcgctcccct tgaggatcgg ggtcgaggcg
     1441 gaagcatcga tgtccatgaa cagcgacggc cccggctggc ccttgaactt cgggctcagc
     1501 gaccagtcct ggtgcgggta gtcgtagacg ccgatgcgcg agaacgagtt ccacttgctg
     1561 aagaggagcc gcgactcgcg ctcgcgcccc ttgatggcgt cgacctcgaa cggccccagg
     1621 ccggcaagct gcatcgtaaa aggcacgccg agcaccacga aggccgcgcg cgcggcccag
     1681 cgcttccagc gcgcgggcgc gaagcacacc gcagccactg ccgcgagcgt ggcggcgctc
     1741 agcaccacgc ccggagcccc gagcaggttc atgagcggca ccagcgcgag gcagccgagc
     1801 gccgccccga gcaggtcgga ggcgtagagc gcgttgatgc gcgcggtgag ccgcgcgaac
     1861 gcgagcgaaa ggaccgtgcc gccggtaaag aacggcgccg ccgccagcag gtagatggcc
     1921 agcatgcgcc acaggttctc ggtcgagtag gtgagcgtga ccgggatgcg gaccaggcag
     1981 gcgagcgcga gcacggtcgc ggtcatatgg gcgatggccg ctatggcgag gagcccgtcg
     2041 gtattgcgcg cagcgagcct gcggcgcgtg aggtagacga acacgccgct cgcggagagg
     2101 ccgaacagcg cgatcgagat cgcgaggaac gcgaagtggt agaacatcgt caccgagaag
     2161 atgcgggtca gcgcgagctc ggtcatgagc agcgccgcgg aagtggcggc gatcgcgacc
     2221 agcgtcgtcc ggttcatagc gcgtcgatct cctgcgcgat cgcgtcggcc accccggcat
     2281 ccatccccag ccacagcggc agccgcagca ggcactcgcc cgcgcgatcg gtgttggtca
     2341 tcgcgctcgc ggtgcggccg tagcggcgcc cggcgggcga gctgtgcagc ggcacgtagt
     2401 ggaacaccga gcggatctcg cgcgcgcgca ggcgctcgat gaggcggttg cggtgctcga
     2461 ggctcggcag caggacgtaa tagagatgcg cgttgtggcg gcagcccgcc gggatcaccg
     2521 gtcgccggat gcgcccggcg cgctcgcagg cctcgaggcg ctcgtggtag cggttccaga
     2581 gctcgatgcg ccgggcggtg atcgcttcgg cctgctcgag ctgggcccag agaaacgcgg
     2641 cgatgatctc gccgggcagg taggacgagc cgatgtccac ccaggtgtac ttgtccactt
     2701 cgccgcggaa gaagctcgag cggttggtgc ctttttcgcg cacgatctcc gcgcgcccga
     2761 tgaaggaggc gtcgttgacc agcagtgcgc cgccttctcc cgagatcacg ttcttggtct
     2821 cgtggaagct gagcgcgccg agctggccga tcgcgccgag gctgcggccg cggtgggagg
     2881 tcatcagccc ctgggccgcg tcctcgatca ccagcaggcg gcggcgctgc gcgagctcca
     2941 ggatcggctc catctcgcag ccgacgccgg cgtagtgcac cgggaccacc gcgcgggtgc
     3001 gcggcgtgac cgcggcctcg atcagcgtct cgtcgatgtt gagcgtgtcc gcccgcacgt
     3061 cgacgaacac cggcacgccg ccgcgcagca cgaacgcgtt ggcggtcgag acgaaggtgt
     3121 acgagggcat gatcacctcg tcgccggggc cgatgccggc gaggatcgcc gccatgtcga
     3181 gcgcggcggt gcacgagtgc gtgagcagcg ccctgcgcgc gccggtctgc ttctcgagcc
     3241 agcggctgca tcgctcggtg aacggcccgt cgccggcgag gcgatggttg cggtgcgcct
     3301 gctcgatgta ggcgagctcg cgcccggtca tgtgcggccg gttgaacggg accttcacag
     3361 gccgcagatt acacttaagg ggaaatgcac tattgtttca tcaccggctt cctgcgatcg
     3421 ggcaccacgc tggtcgagaa gctggtgcac gcgcttcccg gcgcgtgcat cgggccgcag
     3481 ccttttccat ttctctacca cgacgccaag cgcgcctttc tgcgcgcgcg cggcgcaggc
     3541 gaggaacgct acccgctcgg cagcctgttc cgcgaagagc gctaccggcc ggaggagttt
     3601 cacgctttcc tgcgctcgca ctgcctgacg ccggaggcga cggccgcctc ctttgccgcc
     3661 atgcgcggct acagcggctg gaagctgccc gccctggcgg agcacgccgg gcagatcggc
     3721 gagggctcgt ttgccgaggt gtatcggaag ctgtgcgacc gcctgccggt catcctgggc
     3781 gcagaggcga gccgcctgct gggagccaag gaagtattct gcgaggagtt catcccgtac
     3841 ttcctggccg agggcgtgtc ggtcgtcctg gtgctgcgcg acgtgcgcga tgtcctgacc
     3901 tcgctcaagt tcggcagcgg cgcagcctat gccaaccgcg agatgtcggt gctgcacatc
     3961 gtgcgtcagt ggcgcaagag cgtcgcgttc gcgctcgagt tttccggacg gcccggcttc
     4021 gaggcggtgc gctacgagga gctggtcgcg cgcccgcgag cctggctcga cgcgctcgcc
     4081 ggccgatggc gaatcccggc cgcggcgccg ggcgcgatcg aaagcctgcg cgaccaggaa
     4141 gggcgcgaat gggagagcaa ctcgtcgttc gcgccggcgc gcggcgtcag tcgcgactcc
     4201 gtggggcgat acgccgagca cctgccggcc gcgtgg
//
LOCUS       DATBSZ010000129         5337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_564976, whole genome shotgun sequence.
ACCESSION   DATBSZ010000129 DATBSZ010000000
VERSION     DATBSZ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564976"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VIG70_08300"
     CDS             <1..814
                     /locus_tag="VIG70_08300"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEY8250592.1"
                     /translation="RADRYLAELGRHRPDIVAAVTKAMAGAQRDADFVRPERAAFLAV
                     PDDSIDRAVMEKTHDAVVVPARFGWSDVGSWNALWALGAKDALGNVSHGDVMARDSHG
                     SYLRSEGRLIAAVGIENLVVIETSDALLVSTKEHADDVKLAVEQLKRSRRTEHAIHRR
                     VYRPWGYYESIDNGERFQVKRLMIKPGARLSLQLHRKRAEHWVVVSGEAKVTRGDEEL
                     RLAANQSTYIPTGTKHRLENPGTEPLFVIEVQSGDYLGEDDIERFADDYRRT"
     gene            complement(799..1890)
                     /locus_tag="VIG70_08305"
     CDS             complement(799..1890)
                     /locus_tag="VIG70_08305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8250593.1"
                     /translation="MKPLRLVHTEASLGWGGQEIRILSESQGLIGRGHEVTLLCPPEA
                     RIHAEAPRWKVPAVALPIGRKRPLGVKALYGWLKRNPCDVVCTHSSTDAWLVAFALLA
                     LGRPVPMVRTRHISAPVATNAPTRWLYTRATARIVTTGEVLKKELVERNGFPAARIDS
                     IPTGVDASRYRPGDRTAARAALKLPQDRTIVGIVATLRDWKGHEYLIEALAGLPGNVL
                     LVIVGDGPRRELLEKYVDSLKVRPRTIFAGDQADVVPWLQAFDAFSLPSFANEGVPQA
                     LIQAMLAGLPCVATHAGSMAELARDGDTALVVPPKDSAALRAALQKLLADESLRKRLG
                     ESARRHCAANFSYERMLERMEAIYAQVRR"
     gene            complement(1887..3641)
                     /gene="msbA"
                     /locus_tag="VIG70_08310"
     CDS             complement(1887..3641)
                     /gene="msbA"
                     /locus_tag="VIG70_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEY8250594.1"
                     /translation="MDNTKELYFRLLAYVRPYAKVFAIAVLGMVLAAATEPLFPALIK
                     PLLDQGFTPSSERTWPPVAFAAAIIAIFVVRGILTFSSSYCLTWVAQKVVLDLRSAMF
                     SRLVRFPARYFSDNSSGKVLSRVAYDVTGVTDAATGVLTVLIKDSIVIVLLLGYLFYL
                     NWKLTLIALVVGPPIAFTVRVISKRLRRVSREALLAQGDLVHVLQETIDCHKVVKVFG
                     GQDYETRRFRKSAQVLRGNLMRINVSAALTTPITHTLAAFAIAIIIYIALQDSLAGRT
                     TVGEFASFLTAMLMLLAPLKHLSEINNSLQRGLASAESVFGMIDAPVEEDGGTVAIGR
                     SRGEIVLERVSLTYPERTEPALADIDLHVRPGETVALVGGSGGGKTTLANLLPRFYAP
                     TSGRILLDGHDLQSLTLDSLRANIALVSQEIVLFNDSIFANIAYGGMGGAAEQDVIAA
                     AQAAHAMDFIRETPDGLNTPIGENGLRLSGGQRQRLAIARAILKNAPVLILDEATSAL
                     DSESERQVQAALETLMRGRTTLVIAHRLSTIERADRIVVLERGRIVETGRHAELLAQD
                     GIYARLYRMQYAAEMKAA"
     gene            complement(3634..4728)
                     /locus_tag="VIG70_08315"
     CDS             complement(3634..4728)
                     /locus_tag="VIG70_08315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8250595.1"
                     /translation="MRVAIVRQRYNPYGGAERFVARALPALERAGMEMTLISRSEQGW
                     GARRSLRVDPFHAGNLWRDWSFARGARKAWRREHFDVVQSHERIPGCDVYRAGDGVHR
                     RWLELRRAAATPLERLRIALNPYHAYVCKAEKRMLEHPRLRAVICNSKMVREEIRRGF
                     RIAPDKLHVIYNGIDVEQFHPKQRERLRGAARAEIGCSARDTVFAFVGSGFSRKGLDA
                     AIAAVSRGPYWLLVVGRDKYSGRVPERVRFLGGLEDVRPYYAAADCFLLPTRYDPFPN
                     TVLEAMAMGLPAIVSSRCGAAEVIEPGVNGWICQPDDVQGIARLMQEADRANGDGRMG
                     KAARATAERFGIDAMARQLVDLYASLKKLG"
     gene            complement(4725..>5337)
                     /locus_tag="VIG70_08320"
     CDS             complement(4725..>5337)
                     /locus_tag="VIG70_08320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY8250596.1"
                     /translation="QVELNLDALRRIGVQPDLAERSVRFVPGPDAERAIDELVGDVAF
                     VHIHPASRWRFKCWPAQRNAELIDRLAADGHNVVLTSAPDEVGFIDEILKNTAAQPVS
                     LAGKLSIKELGALTARARLFVGVDSMPMHLAAAMGTPTVALFGPSGENEWGPWNVERR
                     VVTTTHTCRPCGYDGCGGGKLSECLTFLPVEPVHAAARELLKK"
BASE COUNT          855 a         1819 c         1854 g          809 t
ORIGIN      
        1 ccgcgccgat cgctacctcg ccgagctcgg gcgccaccgg cccgatatcg tcgcggccgt
       61 caccaaggcg atggcgggcg cgcagcgcga cgccgatttc gtgcgcccgg agcgcgcggc
      121 gttcctcgcg gttcccgacg actcgatcga ccgcgcggtg atggagaaga cgcacgacgc
      181 cgtggtggta ccggcgcgct tcggctggag cgacgtcggc tcgtggaacg ccctgtgggc
      241 gctcggcgcg aaggacgcgc tcggcaacgt ctcccacggc gacgtcatgg cgcgcgacag
      301 ccacggctcg tacctgcgta gcgagggccg cctgatcgcc gcggtgggca tcgaaaatct
      361 ggtggtgatc gagacgagcg acgcgctcct cgtctcgacc aaggagcacg ccgacgacgt
      421 gaagctcgcg gtcgagcagc tcaagcgctc ccggcgcacc gagcacgcca tccaccgccg
      481 cgtctaccgc ccgtggggct actacgagtc gatcgacaac ggcgagcgct tccaggtgaa
      541 gcgcctgatg atcaagccgg gggcgcggct ctcgctgcag ctccaccgca agcgcgccga
      601 gcactgggtg gtcgtgtccg gcgaagccaa ggtcacccgc ggcgacgaag agctgcggct
      661 cgcagccaac cagtccacct acattcccac cggaaccaag caccggctgg agaaccccgg
      721 caccgagccg ctgttcgtga tcgaagtgca gtccggcgac tatctcggcg aggacgacat
      781 cgagcgcttc gccgacgact accgccggac ctgagcgtag atggcctcca tccgttcgag
      841 catgcgctcg taggagaaat tcgccgcgca gtgcctgcgc gccgattctc ccagccgctt
      901 cctcagggat tcgtccgcca gcagcttctg cagcgccgcg cgcagcgcgg ccgaatcttt
      961 cggcggcacg accagcgcgg tgtcgccgtc gcgggcaagc tcggccatgc tgcccgcgtg
     1021 cgtggcgacg cagggcaggc cggcgagcat cgcctggatc agcgcctggg gaacgccttc
     1081 gttggcaaac gaagggagcg agaaggcgtc gaacgcctgc agccagggaa cgacgtcggc
     1141 ttggtcgccg gcaaagatag ttctgggccg gactttcaga gagtcgacat atttttccaa
     1201 cagctctcgc cgcggcccgt cgccgacgat gacgaggagc acattcccgg gcaggccggc
     1261 gagcgcctcg atcaggtatt cgtggccctt ccagtcgcgc agcgtggcga cgatgccgac
     1321 gatcgtccgg tcctgcggca gcttgagcgc tgcgcgcgcc gcggtgcggt cgccggggcg
     1381 gtagcggctg gcatccacgc cggtcggaat cgagtcgatg cgcgccgccg ggaagccgtt
     1441 tcgctcgacg agctccttct tgagcacctc gcccgtcgta acgatgcgcg ccgtggcgcg
     1501 ggtgtagagc cagcgcgtgg gcgcgttggt ggccacgggg gccgagatgt ggcgcgtgcg
     1561 caccatggga accggacgac cgagagcgag cagcgcaaag gcgacgagcc aggcatcggt
     1621 cgagctgtgc gtgcacacga cgtcgcaggg gttgcgcttc agccacccgt aaagcgcctt
     1681 gacgccgagc gggcgcttgc gcccgatcgg cagcgcgacc gccggaacct tccagcgcgg
     1741 cgcctcggcg tggatacgag cttccggcgg gcaaaggagc gtgacctcgt ggccgcggcc
     1801 gatcagcccc tgcgactccg agaggatgcg gatttcctgg ccgccccacc cgagcgaggc
     1861 ctcggtgtgg accaggcgaa gcggcttcac gccgccttca tctcggcggc gtactgcatc
     1921 cggtagagcc tggcgtagat gccgtcctgc gccagcagct cggcgtgcct gccggtctcg
     1981 acgatgcggc cgcgctcgag taccacgatg cggtcggcgc gctcgatggt ggagagccgg
     2041 tgcgcgatga cgagggtggt gcgcccgcgc atcagcgtct cgagcgccgc ctgcacctgg
     2101 cgctcggatt cggagtcgag cgcggaggtc gcctcgtcga ggatcagcac cggggcgttc
     2161 ttgaggatcg cgcgcgcaat ggcgagccgc tggcgctggc cgccggagag acgcaggccg
     2221 ttctcgccga tcggcgtgtt caggccatcg ggcgtctcgc ggatgaagtc catcgcgtgc
     2281 gccgcctgcg cggcggcgat cacgtcctgc tcggcggcgc cgcccatgcc gccgtaggcg
     2341 atgttggcga agatcgagtc gttgaacagg acgatctcct ggctgaccag cgcgatgttg
     2401 gcgcgcaggc tgtccagagt gaggctttgc aggtcgtggc cgtcgagcag gatgcgcccc
     2461 gaagtcggcg cgtagaagcg cggcagcaga ttggcgagag tggtcttgcc gccgcccgag
     2521 ccgccgacca gcgccacggt ctcgccggga cgcacgtgca ggtcgatgtc cgccaacgcg
     2581 ggttccgtgc gctccggata ggtcagcgag actctttcca ggacgatctc gccgcgcgag
     2641 cggccaatgg cgaccgtacc gccgtcctcc tccaccggcg catcgatcat gccgaacacg
     2701 ctctccgccg aggcgaggcc gcgctgcagc gaattgttga tctcgctcag gtgcttgagg
     2761 ggcgcgagca gcatcagcat cgcggtgagg aaggaggcga actcgccgac cgtcgtgcgg
     2821 cccgcgagcg aatcctgcag cgcgatgtag atgatgatgg cgatggcgaa agcggcaagc
     2881 gtgtgggtaa tcggggtggt cagcgcggcc gagacattga tgcgcatcaa attgccgcgc
     2941 agcacctgcg cgctcttgcg gaagcgccgg gtctcgtagt cctggccgcc gaacaccttg
     3001 acgaccttgt ggcagtcgat ggtctcctgc agcacgtgca ccaggtcgcc ctgggcgagc
     3061 agcgcctcgc gcgagacgcg gcgcaggcgc ttcgagatca cccgcacggt gaacgcgatc
     3121 ggcggcccca ccaccagcgc gatcagggtg agcttccagt tcaggtagaa caggtagccg
     3181 agcagcagca cgatcacgat ggagtccttg atcaggaccg tcagcacgcc ggtggccgca
     3241 tcggtcacgc cagtgacgtc gtaggcgacc ctggagagga ccttgccgga gctgttgtcg
     3301 ctgaaatacc tcgccgggaa gcgcaccagc cggctgaaca tcgcgctgcg caggtcgagc
     3361 actacttttt gcgccaccca ggtgaggcag tagctcgagc tgaaggtcag gatcccccgc
     3421 accacgaaga tggcgatgat ggcggcggcg aaggcgacgg gcggccaggt acgctcgctg
     3481 ctcggcgtga agccctgatc gagcagcggc ttgatcagcg ccgggaacag cggctccgtc
     3541 gccgccgcca gcaccatgcc gagcaccgcg atcgcgaaca ccttcgcgta cggccgcacg
     3601 taggcgagca gccggaagta gagttctttg gtgttatcca agctttttta gagaagcata
     3661 caaatcgacc agttgccgcg ccatggcatc gatcccgaag cgttctgccg tggcgcgcgc
     3721 ggcctttccc atgcggccat cgccgttggc gcggtcggct tcctgcatca gccgggcgat
     3781 gccctgcacg tcgtccggct ggcagatcca gccgttcacc cctggctcga tcacctcggc
     3841 cgcgccgcag cgcgagctca cgatcgccgg caatcccatc gccatcgctt ccagaacggt
     3901 attgggaaac ggatcgtagc gggtcggcag gagaaagcag tccgcggcgg cgtagtaggg
     3961 gcgcacgtcc tcgaggccgc ccaaaaagcg cactcgctcc ggaactcggc cgctgtattt
     4021 gtcccggccc accaccagca gccaataggg ccccctggag actgccgcga ttgccgcgtc
     4081 cagccccttg cgcgagaacc cggagccgac gaaggcgaac acggtgtcgc gcgcgctgca
     4141 gccgatctcg gcgcgcgccg cgccgcgcag gcgctcgcgc tgcttcgggt ggaattgctc
     4201 gacatcgatg ccgttgtaga tcacgtgcag cttgtcgggc gcgatgcgga agccgcgccg
     4261 gatctcctct cgcaccatct tcgagttgca gatcaccgcg cgcaggcgcg gatgctcgag
     4321 catccgcttt tccgccttgc acacgtaggc atggtacggg ttgagcgcga tgcgcaggcg
     4381 ctcgagcggc gtcgccgccg cgcgccgcag ctcgagccag cggcggtgca cgccatcgcc
     4441 ggcgcggtag acgtcgcagc cgggaatgcg ctcgtgcgac tgcacgacgt cgaagtgctc
     4501 gcggcgccag gccttgcgcg ccccgcgcgc gaacgaccag tcgcgccaca ggttgccggc
     4561 atggaagggg tcgacgcgca gggagcggcg cgcaccccac ccctgctcgc ttctggaaat
     4621 caacgtcatc tccattccgg cgcgctcgag cgccggcagc gccctggcga cgaagcgctc
     4681 ggccccgccg taggggttat agcgttgccg cactatggca actctcactt cttgagtagc
     4741 tcacgcgccg cggcgtggac gggctcgacc ggcaggaacg tgaggcactc ggagagcttg
     4801 ccgccgccgc agccgtcgta gccgcacggc cggcaggtat gcgtcgtcgt caccacgcgc
     4861 cgctcgacgt tccacgggcc ccactcgttc tcgccgctcg gtccgaacag cgccacggtc
     4921 ggcgttccca tcgccgcggc gaggtgcatc ggcatcgagt caacgccgac gaacagcctc
     4981 gccctggcgg tgagcgcgcc gagctccttg atcgacagct ttccggcgag gctgaccggc
     5041 tgggccgcag tatttttcag gatctcgtcg atgaagccga cctcgtcggg ggccgaggtg
     5101 agcaccacgt tgtgcccgtc ggcggcgagg cggtcgatca gctcggcgtt gcgttgcgcg
     5161 ggccagcact tgaagcgcca gcgcgatgcg ggatgtatgt gcacgaacgc aacgtcgccg
     5221 accagctcgt cgatcgcccg ctcggcgtcc gggcccggca cgaagcgcac actgcgctct
     5281 gcgagatccg gctgcacgcc gatgcgccgc agcgcgtcga ggttgagctc cacctgg
//
LOCUS       DATBSZ010000130        32391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1657542, whole genome shotgun sequence.
ACCESSION   DATBSZ010000130 DATBSZ010000000
VERSION     DATBSZ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 32391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32391
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657542"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1237
                     /locus_tag="VIG70_08325"
     CDS             275..1237
                     /locus_tag="VIG70_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250597.1"
                     /translation="MKVLLLVAQLVLVAAAVAQETDYPSRPIKQIVPFPPGGGVDIVT
                     RIVGAKWSEFLGQPIVVENRAGAGGTVGADVAAKAAGDGYTLLTCQVASHGVSPALYN
                     KLPYDHVRDFAPISLIGKTPNVLVVHPSVPARTLKEFIDYVRASPGKYNYASPGYGTS
                     PQMTMELFKLTTGLDMTHVPYKGGAPALADVMGGQVTGMFGNLPEQLAAIKGGKTRAL
                     AVSTLSRSPQLPDVPTVAESGFPGFEVTVWYGVCAQSSVPKPILAKLHATLLKTLNLP
                     EMKARLAESTIDASPTTPEEFAAFIRAETEKWTRVVRDANIPKQ"
     gene            complement(1239..1874)
                     /locus_tag="VIG70_08330"
     CDS             complement(1239..1874)
                     /locus_tag="VIG70_08330"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY8250598.1"
                     /translation="MAVLRIAKMGNPILLQKAAQVVDPTAPEIRRLAADMQDTIEDIG
                     ASGLAAPQVFVGKRVVVYRIIAARIPPGSGLQPRPWTVMVNPVITPKSDAKTPVWERC
                     LSVPGLHGKVPRHMHVQITYEDLDGRAHSHEAASSWAALLQHECDHLDGIVYPMRMTD
                     LSLLAYNDEPGPLAREISKGEKNIDPLFIDLVERWPGRARWFQKMGTDPIS"
     gene            complement(1896..2378)
                     /locus_tag="VIG70_08335"
     CDS             complement(1896..2378)
                     /locus_tag="VIG70_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEY8250599.1"
                     /translation="MGNRNSSRQAGFTLIEVMVVTAVIGILASILMPQVRSYTARTKI
                     SEAVLALTNCRNIIHEVYLSGSDIPGADNWGCEVERPSRFVERIRTTDEGVIRLTLGN
                     EIGDLRLAIHDITLAPLNGSGQIMREEDLGTPVRRWRCGATVDGTDVKAEFLPSTCRG
                     "
     gene            complement(2545..3126)
                     /locus_tag="VIG70_08340"
     CDS             complement(2545..3126)
                     /locus_tag="VIG70_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250600.1"
                     /translation="MAEPRMVDPHDPVMTGENSFVRLSNDGGTTIPERVSHWRVLWSP
                     AGQGHALFIESSLLKNRIYADNAGVARYLQRTIEVLLHKPFADESLPIVDARFERSGN
                     SLSTVEERVVSSKDEIILTWWDFMAPFVLTMPPGMMNRPLGVYSTFIPARGAQLSVNG
                     QAAPGKVFPQERGGRQSSSCVLAWSETWTRPKG"
     gene            complement(3129..3422)
                     /locus_tag="VIG70_08345"
     CDS             complement(3129..3422)
                     /locus_tag="VIG70_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY8250601.1"
                     /translation="MAKAYWVVCYRAIKNQDKLAAYAKLAGPAIQAAGGRFLVRGTPS
                     KTYEGGLNQRTVVTEFESLAKAIAAHDSPGYQEALRALGDAADRDLRIVEGVS"
     gene            3481..4602
                     /locus_tag="VIG70_08350"
     CDS             3481..4602
                     /locus_tag="VIG70_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY8250602.1"
                     /translation="MTTQDAVSLTKALLRFDTVNPPGRERDCARYAGAMLQEWGFKVD
                     YHEYADSRTSVIARAGGSDRKAPLCLTGHLDVVPLGARAWTRDPFNGDTDGDRLFGRG
                     ASDMKAGVAAILLASRTFSGKLSGTPGVVIVLTAAEEGGCVGSAQLARTQLLGKAGAM
                     IVGEPTSNYPLVGHKGSVKFHARFKGVSAHGSMPELGVNAIYKAGKALASLEGFDFAT
                     PEHPVMGKPTLNVGTIEGGNTVNAVPDAASIGVDIRTVPGMDHEALLRKIRASIPEAE
                     LEVFSNLKPVWTAPEQEWIQRVFELCKPYLSETPAARTAPYMTDAANLLKVYAGAPTV
                     VLGPGEAAMAHQTDEYVSQERIRQSVAIYESLIRDWCRP"
     gene            complement(4670..4978)
                     /locus_tag="VIG70_08355"
     CDS             complement(4670..4978)
                     /locus_tag="VIG70_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250603.1"
                     /translation="MIRFTPVALVAVAAALGACGTPPQSAQPNIVTNVHPYKAGNGVV
                     QSVLAVPVLPGAGSSAQGLQRLEIKMDNGAVQYVDTPSREFSRGTRVSLSEDHVIRRM
                     "
     gene            5055..5846
                     /locus_tag="VIG70_08360"
     CDS             5055..5846
                     /locus_tag="VIG70_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8250604.1"
                     /translation="MGSSSPPWVRAETVDIAVDRQRIEGTLVAPTVRRDEVPGVLLVH
                     GWNGTPAGYLDEAHALAAALGCVCLTFDLRGHGQSEAQRETLTREDNMRDVMAAYDVL
                     AGRQRVQKSCIALVGSSYGAYLGALLTYMRPVHLIALRAPALYTDDHWEMPKRKLRVD
                     REFAAYRRRSLRAEQNRALGACAQFLGDALVVESARDEVVPHAVVANYIAALANAHSL
                     TYRVIEGADHALSQEPWERAYIELLVSWLSEMIRGAAGPAPAGAR"
     gene            complement(5806..6087)
                     /locus_tag="VIG70_08365"
     CDS             complement(5806..6087)
                     /locus_tag="VIG70_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250605.1"
                     /translation="MALAGAALGQSGERGSIPPGQSRDGAAPSDGAIKGGTILPGETG
                     GMPGKRCEELSGTLREECLKQEREAASGETQRPGERPSALPRERDPRRP"
     gene            6157..6867
                     /gene="pepE"
                     /locus_tag="VIG70_08370"
     CDS             6157..6867
                     /gene="pepE"
                     /locus_tag="VIG70_08370"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HEY8250606.1"
                     /translation="MSTVGMELLLLSSSRTPAGYLTDYLAAIRAFAGGIRAAHFVPYA
                     AVSLPWEEYCSSVGKALAAADLRVELVDSVDDLMRAELIVVGGGNTFQLLRECRRRGF
                     LPVIQERVKAGTRYLGWSAGANLACPTIKTTNDMPIVDPGSLDALGLVGFQINPHYLS
                     VALPGHHGETRDERLAEFARVNPKLPVLGLPEGASLRVSDSTVELLGPYPAVLFAGEK
                     SARRLMPGKLPTLSDDSA"
     gene            6964..7290
                     /locus_tag="VIG70_08375"
     CDS             6964..7290
                     /locus_tag="VIG70_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250607.1"
                     /translation="MEKTAVLRLISLIPDRVSRSQSGSTFVGTGAAGRAQLLAPFLSW
                     LGPLNPISKEEQQILGVRSGGGKALVDGEAAGEEVEEAADRVELADEVDDSPIRLPGT
                     GTQIAA"
     gene            7319..7507
                     /locus_tag="VIG70_08380"
     CDS             7319..7507
                     /locus_tag="VIG70_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEY8250608.1"
                     /translation="MLSWAIGFFILALVAAFFGFGGIASSAAGIAQVLLVLFVIAFIV
                     SLILGLRGRGGVTRRDLF"
     gene            complement(7714..8097)
                     /locus_tag="VIG70_08385"
     CDS             complement(7714..8097)
                     /locus_tag="VIG70_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250609.1"
                     /translation="MTFTKLVLYTDRDGRAKWREEAIALPEGTPQAMLSRVFPSAGYQ
                     LRHSPVGFRSQWHCTPQPQWVFILAGEMEIGLQDGSSRVFKAGEHFFSADTLPAGAAF
                     DAKVHGHWSAQRGASPLVTLFVKAP"
     gene            8136..9353
                     /locus_tag="VIG70_08390"
     CDS             8136..9353
                     /locus_tag="VIG70_08390"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEY8250610.1"
                     /translation="MRMKWIATGLLVLAALVYVVSEKFDWYYLAAFSEAAMVGALADW
                     FAVVALFRRPLNLPIPHTAIIPRNKDRIARGLSDFIEQNFLSSAAVVQRIAEFRPAHT
                     LCQWLLKPDNVDTVAGYATRFVAYALSAVDDERVRRFLHQSIIGMSKNVDFAAAAAQV
                     LDVLTENKRHHALLDAALRGLDDLLAKEETRRFIAVEVAKSAPLLKRISDWLQLDLDE
                     RAALKIAELAIAKVHEVRQDGDHELRRRFDDFVAEFIGRLKTDESLRKRVAAIRDELL
                     ESPALGSYIGSLWQDFRAWLSADLGAGQSVAHGRIAAMVRAFGARLEADREIRQWIDE
                     QILTAIPPLVEEHRAKIGRFVEDQINGWQEEKLVEELERHIGPDLQYIRINGTLVGGL
                     AGLAIAALTQVAK"
     gene            9444..9617
                     /locus_tag="VIG70_08395"
     CDS             9444..9617
                     /locus_tag="VIG70_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250611.1"
                     /translation="MKNCDIHHVKHKNSFRWKWRHTRSDGTVKESAESYKLYYECVMA
                     ARASGYEPRLKCA"
     gene            complement(9622..9882)
                     /locus_tag="VIG70_08400"
     CDS             complement(9622..9882)
                     /locus_tag="VIG70_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250612.1"
                     /translation="MPFALYYASSPPPKSLDRETLQRLVPVHFSTEQDACHAAALVLR
                     GGQHVWLIEGPDVRMTAAEIAEKCRPTLEMFKRTRRPDGEKR"
     gene            complement(9930..10211)
                     /locus_tag="VIG70_08405"
     CDS             complement(9930..10211)
                     /locus_tag="VIG70_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250613.1"
                     /translation="MRAIVAFICIGLALPAVAQQRAPDIDPNTKIQGGADVRGSSAAA
                     GTGAGAGAQSDGKKENEPSDKNAPEKDKPTSERKPEEAAKDEPPRPRQF"
     gene            10241..10399
                     /locus_tag="VIG70_08410"
     CDS             10241..10399
                     /locus_tag="VIG70_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250614.1"
                     /translation="MRPFWILAFALAVLAACDRAATDRAANEGRAAQSAAGGSANEGK
                     AARSAAGR"
     gene            complement(10396..11472)
                     /locus_tag="VIG70_08415"
     CDS             complement(10396..11472)
                     /locus_tag="VIG70_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY8250615.1"
                     /translation="MKVTAVDTCVLTVPTPKPMALQYPQHRLVVAQISTDQGVRGLGY
                     SLVFGGGGAEAVHAYLEARLKPALIGEDPLYVEYLWEKMFRVDMGMKKQGVAAYALAA
                     LDIGLWDIVGKALGKPLYKVWGAMRHRIPCYGSGGWAKYSVDDLVREAEKFAGFGCKY
                     YKMKIHDPDPKVNRVRVEAVKRALGPNVRLMVDVNQRLDVLGNVRQAAELEHLDLVWY
                     EEPVLADDIASCAEVSQRIRIPVATGENNFTRFEFRDLLERRAARYLMPDVCRANGFS
                     ETLKIGHLAAAHGALVSPHVVHEISLHVVGALANGFLVEFMEWAPSDLFEEQPECKGG
                     RFRIPDRPGHGMSLARGAIDKYRR"
     gene            complement(11490..11693)
                     /locus_tag="VIG70_08420"
     CDS             complement(11490..11693)
                     /locus_tag="VIG70_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250616.1"
                     /translation="MARLLTLLLCGLAAASIDARAANDDDGFVWIDANTRIKVLKPVS
                     AAAGESKAPEEAKKEKKAEKRKK"
     gene            11749..12867
                     /locus_tag="VIG70_08425"
     CDS             11749..12867
                     /locus_tag="VIG70_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250617.1"
                     /translation="MNVHVRIASAVLFLAACAQTPPGGGELPAFRADPNWPRPLAEEN
                     GVQLVLGQVAGIAVNPSNGHVWIVHRPATLLPDEWNAKESKPVTHRCCKSAASVIEFD
                     AAGNYVRGWTPSGAGFDWPKTEHGIHIDSEGNVWLAGNGNEDHQILKFSPEGKFLLQI
                     GRPGKSEGSNSRTQLGRPAHMVLSGGELFVADGYGNRRVVVFDAKTGAYQRHWGAYGT
                     ATPSDDKLPSYDPAQPPSKSFGNPVHCVRVSNDGLVYVCDRQNNRIQVFSRTGEFRQE
                     IRVEVQTLANGSTWDMVLSHDPAQKYLYVADGANGRIYIVRRSDGQTVGAFGRTGRMA
                     GEFKWIHNIAIDRDGNLYTSEVGFGRRVQKFVRSGGAF"
     gene            12912..13640
                     /locus_tag="VIG70_08430"
     CDS             12912..13640
                     /locus_tag="VIG70_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY8250618.1"
                     /translation="MTVRLVSSVEIVRIAKTAGFDAIYVDLEHSAFSLETTGQLSVMA
                     LEAGVPALVRVPARTPEYISRVLDGGALGVIAPGVDSAAEAKEVVAAAKYPPLGSRGL
                     STGLAHLGFRAVPAAEALPALNEATMVIVQLESAAALAAIDDIVAVEGVDMVLIGTND
                     FLADLGHPGEYDHPKVREAYERTIAACRKHGKHVGVGGLSSRPDLVQKFVAMGARFVS
                     TGTDLGFLLAACTERARQVRNLKI"
     gene            13652..14605
                     /locus_tag="VIG70_08435"
     CDS             13652..14605
                     /locus_tag="VIG70_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250619.1"
                     /translation="MRKGFLLAALLFAASAFGQYPAKPVRLIVPLVPGGNQDIVARVV
                     AEELSKGLGQQVIVENRPGQSAIIGTQFVKSAPADGYTLLSVAVTFARVPAVVKAAGY
                     DPAADFAGVSLVCRIPQVLVLTPSHPAKNLQELIALAKAKPGEVSFGSSGNGSTGHVA
                     AEMFMRMTGTRLLHVTYKGNAQAIVDVMGGQVAMMFDQVSTSGSHVKGGKLRALGVTT
                     KARSAVLPEVPTLHEQGLTDFEDVTWNGIVAPAGTPRDVISKLHATVARALASPELQK
                     RYGERGIEIVASRSPEEFSAYVKSEADAFARLVRDAGIKVE"
     gene            14602..15489
                     /locus_tag="VIG70_08440"
     CDS             14602..15489
                     /locus_tag="VIG70_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY8250620.1"
                     /translation="MSPEIIQTEVFAELPEKLRKSGSPPERLAAGKSATPMGSFLEGP
                     VFDRAGNLYVVDLAWGRIFKVNPKGEFELVIEYDGEPNGLAMHKDGRLFIADHKNGIL
                     YLKQGKLVPVVSRYHQQAFKGVNDLMFDPEGNLYFTDQGVTDLADPTGCVYRYSADGK
                     LEQLANCLPSPNGLVVDGRMIYVAVTRANAVWRLPLTPDGKVVRLGVFLHLSGGRGPD
                     GMAMDESGGLAVAHIDFGAVWIFSHRGEALYRVASCRSDIVTNVAYKGNEIYITDSGS
                     GCILRARVPTAGRALFSHL"
     gene            15486..16433
                     /locus_tag="VIG70_08445"
     CDS             15486..16433
                     /locus_tag="VIG70_08445"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250621.1"
                     /translation="MKILISLLLLWLGTAQAFPTKPVRIIVAFPPGGGTDIVARLLAP
                     RLGDLWGQPVVVENRAGASGTIGTEIAARSDPDGHTLFMATMGNMTANQHLYPKMAVD
                     PLRAFAPITKVVDVHFVFMANPALPADNVRDLVEAAKKRPGELHYSSSGPGGAPHLAM
                     ELFKRQAGINLTHVPYKGSGPGMTDLLGGRVMMTMDSLLQGLPQIKAGKLKALAVLGP
                     KRSSLLPDVPTMAEAGVSGYALTNWFGLLAPAATPKEVLAKVHADVTKILRETEIQKR
                     IADMGADVVGNSPEEFGAAMRAESAQWADVIKSANIKAE"
     gene            16430..17263
                     /locus_tag="VIG70_08450"
     CDS             16430..17263
                     /locus_tag="VIG70_08450"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8250622.1"
                     /translation="MKVAAEKVCDSHFHVFGPAERYPYAGDLRYTPPHAPLNEYLKLA
                     RDLGIEKHVFVQPSAYGRDNACMLDAMREVGPARCRGIVDIDEDASDATLRDLNAAGV
                     RGVRVNVNPIKPAEPGFFRSLLPRIEKLDARCAERGWMLDFLTPGWLTRQLFPTFAKL
                     ASPFSIAHMGMFLANGGAGQPGFLELLDFCKTNPRCWVKLTGVYRVSTAPGFADAAPL
                     AQALIAAIPDRLIWGSDYPHLSFGHVHTDELFGLLKSWAPDAAAQRRILEDNPARLFG
                     F"
     gene            17292..17540
                     /locus_tag="VIG70_08455"
     CDS             17292..17540
                     /locus_tag="VIG70_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250623.1"
                     /translation="MKTTLLTIVIALAVAGCDRTPDQKPPQAGAGASAPQSNTPSTPA
                     NAGTPSAAEKKEGANPVQGQVDPKQPEQRKDFQQKSGG"
     gene            complement(17537..18592)
                     /locus_tag="VIG70_08460"
     CDS             complement(17537..18592)
                     /locus_tag="VIG70_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250624.1"
                     /translation="MLVRLDYPPPVEYPLTTTVVGSYPQPGWLIDRAQLGSKVPRVRM
                     PEIWKVEKTFLEEAQDDATLLAIRDMERAGIDIITDGEMRRESYSNRFATTLEGIDIE
                     RPGSTINRSGVAIPVPRVAGPIRRRAPVEVRDVEFLRKNTSRRIKITLPGPFTMAQQA
                     QDDYYKDEEALALAFAAAVNEELRDLKAAGADVVQLDEPWMQARPDRAARYGVKAINR
                     ALQGIPGTTVVHVCFGYAAAVKDKPTGYSVLPQLEALTASQVSIEAAQPKLDLGVLKT
                     LGSKTLMVGMIDLGTERIETPQEVAARIRAALKHVPAERLVLAPDCGMKYLTREAAFG
                     KLKALAEGAAIVRKEIS"
     gene            18655..19050
                     /locus_tag="VIG70_08465"
     CDS             18655..19050
                     /locus_tag="VIG70_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250625.1"
                     /translation="MRLSALAVAVSLALGAGMLTGCDRQDGDRTVSKSPGSSSSSAGS
                     TTPSSPSSSTSGSASVSTTPSTPPSSSASSSADATKKDSASGGTSSSSSSSSPSSSPS
                     GSAASGSSSPSSASSSSAAQDPAKEEKKQ"
     gene            complement(19151..20362)
                     /locus_tag="VIG70_08470"
     CDS             complement(19151..20362)
                     /locus_tag="VIG70_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY8250626.1"
                     /translation="MSQKLPVVIAGAGPTGLMCALALARRGVPVVVCEGEPSLTRDLR
                     AGTFHPPTQEMMAPYGITDQMHAHGLQVRRWQIRSRRDSLVAEFDLGLLADVTPYPYR
                     LHLEQHRLTPIQLDILRKEASAEVHFGHRITGFEHKARSVLVRFESEGIPGKLEASWL
                     IGADGGRSTIRRLLPVEFEGFTWPEQFLVVSTPYDLGRHGFTLNAYVADPVEWAAVFK
                     MPDAGPPGLWRLAFPCDPGLPDDALLDPGAVQKRMQGFLPRDSSYEIRYQSIYRVHQR
                     VASEWRLGRVLLAGDAAHLNNPLGGFGLNSGIHDAVNLADKLARVWHGEADEALLDVY
                     VRQRRATTIEQVQAMSIRNKRLLEERDPQVQRSRMEELVAVAADPQRARQHLLESSMI
                     AGLRKNSGTEP"
     gene            20384..21391
                     /locus_tag="VIG70_08475"
     CDS             20384..21391
                     /locus_tag="VIG70_08475"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY8250627.1"
                     /translation="MRICIFGAGAVGGHFAAKLAASGHDVSVVARGAHLEAMRNNGIT
                     LVHGNDTIRGRVRAAASAAELGLQDFVFVTLKANLLGVFAEQAAAIVGADTGVVFAQN
                     GIPWWYGIGLAASEASKGRPPPPDLSRLDPGGKLKKFLEKKQIIGGVVYSANEVREPG
                     VIVNNVPGNNMLVVGQADDSDSQAIGALRSILDKADLYSPPTSDIRQVVWAKLVQNLS
                     TAALCTLAGATVKEVREDPSLGELSKRLAAEARAIALAHGIDAAKAPQRPGGGQSSGA
                     ISHKPSLLQDYERGRPMEIDALLIAALEFGRAAKVPAPALETVVPLVVFKAAAKGLYD
                     H"
     gene            21405..23684
                     /locus_tag="VIG70_08480"
     CDS             21405..23684
                     /locus_tag="VIG70_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8250628.1"
                     /translation="MRLKTPIEAPSLEAALELLERLPVPMYVEGRDGRCLGVNAAWEE
                     LFGIKRERVLGKGVRELCPQNPEIAEKHAIKDRELWAQPGSQSYDIPVVTPDGRRRET
                     VYYKSTFPPGPEPAGLVGAIFDVTARKRAEIALRESEERFRAVVDSANEGMLVYDRRL
                     LVVSVNRAAERILRLPASELIGKPGFTSLLPCISEDGAPLGPDERPTLTTVHAGQPQT
                     DRVVGVKRSDGSVTWLSVSTTFLRRMDETEHYGLVSTITDITAQHDAEQRLRESERRF
                     RNTFELAGSGMAHIGMDRRFIRVNRRLCEILGYPEAELLQLTGRQISHPDDLDVINEQ
                     RPRLHSGEVDAVRLEKRYLRKDGSVVWVKFAMTVERDAEGRPLYEIAVYDDITAQLDA
                     QLRLRESEARFRQTFELAASGICHVIDGRFVRVNRSLCEILGYSEKALVGKHVKDVSH
                     PDDRDVTDAGRARVRRGEIEAARFEKRYIRGDNGAVVWADIAVAVVRDVFGVPQYEVA
                     VFDDITERKHAEAAARAAHQELERSNAELEQFAYVASHDLQEPLRMVASYTQLLSRRY
                     DSKLDKDAREFMAYIVDGATRMKQLIEDLLAYSRVGTKGADFRPVAAEAALRRALFNL
                     RAGIEEAGAVVTHDPLPTLPADEVQLAQVFQNLIGNALKFRSASVPRIHIGVAEGPRE
                     FTFEVKDNGIGIEPQYYERIFMVFQRLHNKGEYPGTGIGLAICKKVVERHGGRIRVES
                     RLGSGSSFYFTLPKERGEG"
     gene            23684..24133
                     /locus_tag="VIG70_08485"
     CDS             23684..24133
                     /locus_tag="VIG70_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8250629.1"
                     /translation="MVERNGPIEILLVEDNPGDVRLTREALKEGKVYSNLHTVKDGVE
                     AMEFLRRQGKYKDVPRPDIILLDLNLPRKDGREVLEEIKSDSVLKRIPVVVLTTSKAE
                     EDVLRTYNLHANCYVTKPVDLEKFMVVVKTIDVFWLTVVTLPPNGHS"
     gene            24156..25838
                     /locus_tag="VIG70_08490"
     CDS             24156..25838
                     /locus_tag="VIG70_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8250630.1"
                     /translation="MEDNPGDARLIQEMIREDPGAPFQVHCADRLSRGLEHLAGGETG
                     LVLLDLSLPDSMGLETFSRVYAHSPAVPIIVLTGNDDQKTALSAVKGGAQDYLVKGRL
                     DRELLLRAMHYSIERKRYQVQLEHQANYDALTGLPNRNLLHDRLRQAVYSQRQSRAIA
                     VVFMDLDHFKFVNDSLGHSTGDLLLKAMGERLRTVLREGDTVGRVGGDEFVLILNDQS
                     NEEIIYRAMQRISAKVSEPLIIDGKELHVTCSAGISLYPQDGRDVDTLLKNADAAMYR
                     AKDHGRSNFQFYTSEMNERVSERLALENALRRALERNELALHYQQKVNLQTGEIVGAE
                     ALVRWLHPEWGLVRPARFIPLAEETGLIVSIGEWVLREACRQTRAWLDAGLDPGVVSV
                     NLSLRQFRQEGLVRSVSRILEETALDPAQLEMELTESMVMGNVDAAIAVLQGLKQLGI
                     TLSVDDFGTGYSSLSYLKDLPIDTLKIDRSFVRDIGSGAELDEGVLAQAIISLGHSLH
                     LKVIAEGVETDAQVRFLRRHGCDEVQGFFYGEPVAPEAHARLLEKARRRARN"
     gene            25901..26170
                     /locus_tag="VIG70_08495"
     CDS             25901..26170
                     /locus_tag="VIG70_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8250631.1"
                     /translation="MSERDTAKAQLVHKIGEIIRHNGFTQVQAAKILGLTQPKLSGIL
                     RGRFRGVSERKLIDCLISLGRDVEIVVRDAPGRRAGGKLTVVFPE"
     gene            26167..27360
                     /locus_tag="VIG70_08500"
     CDS             26167..27360
                     /locus_tag="VIG70_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8250632.1"
                     /translation="MSDGEKKAGPVILAVDDSEDMLALVTRALGEEYQVMTAADPGSA
                     IEKAFGEPRPDLILLDIDMPDIDGFEVCRALKGEPATSAIPVIFLTGRSEPQSQAEGL
                     ALGAVDYISKTGTNATILKSRVGMHLALVNQRAELERLVLERTEQLERTRVEMIKRLA
                     RVLEVHESQAVGNRVMRLAHYAKLIAQGSGALPDMTEMIQKAAPLHDIGKLGVPAEIL
                     RKREKLSAPDWERIKRHPEIGAEIIGEHTDPLLRLARQLALTHHENYDGSGYPKGLKG
                     EVIPWGGRVMAIVDSFEAMTSTQFYREALPIERASGEIERGAGSKYDPKLVEAFKKAL
                     PVMQKVRETYSDQLGDLINLDFAPKVAAKPAPPAPAARPAAPPPAAKPKLSAAEMVKA
                     AARKK"
     gene            27370..29799
                     /locus_tag="VIG70_08505"
     CDS             27370..29799
                     /locus_tag="VIG70_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250633.1"
                     /translation="MSKIFDKLKGAERERIEREKSGEEVAQARAGEERRGLDLARERQ
                     AAEVELRRLAHSRAEAEAKAARLVTERERAEAAAAAEARQREEAEKKAAAIAEGRKKK
                     EEEAAQHAKARAESEAQAAAAMKQRAANEEQARRAARERAHGEAKALEAARERKTAED
                     KQKREAETRVAAELEAGRLAQERVATERAASEQAQGRIAAETRASALAEERRRVETAA
                     AELARARAEAEEQARVAAEERIAAERNATAALREREAQDAHAAKHAQARADAEAAALQ
                     SAAERKEAEERALEEAKAKIEAANRATQLAEERRALEQAADAKARKRAEVETQALEAA
                     KRQASAEDAARQAAETRAHVETEAARLAREREIADGELKRIAHERLAAEADAAQAARE
                     AEEAKARAAAEAGERTAAEQALAAAALERHEADAALSERSKALLSAESIALQAARERE
                     QAETAARKAAEDRAQAEVDAARVAREREKAESQTEAALAARIEAEDRRRRAAEELAAA
                     EGAAAAELQGRIAAERALEAEARRAGDAEKQAVDAAAQRAALDAELRRGAQARAEADA
                     AAARRAAEREHAEAKAASDHEMRLEAERKSERISLEKAEAESRATATLQARIAAEEAL
                     TRKQVEAEAAARQAEEAARARAEHEEELALALAARAAADAAAAKAKAVQLRADEKALA
                     ALQKKLDAEKKLELAAGRRAEVESAAADLLRAGIAAEEALEQQTHRAADASARVVSTK
                     EERIALKARLAQLRPRRLPLVAAGLAIGVALLAAFVFVVTQEPPVPASFQPVGEPLKL
                     KLEFAFPAARR"
     gene            29839..31725
                     /locus_tag="VIG70_08510"
     CDS             29839..31725
                     /locus_tag="VIG70_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEY8250634.1"
                     /translation="MERDRSGKDTQTALTLAALGVVFGDIGTSPLYAVKETFSPAHGL
                     PFTPATILGGLSTIFWSLIIVVSLKYVVLVMRADNRGEGGTMALLALASNALKQRPRQ
                     RLAVTLLGLLGASLFLGETVMTPATSVVSAIEGLQVGTPLFERWVVPLSVLVLFGLFA
                     LQRLGTAKVGALFGPVCLLWFLALGAVGLWNIWLNPGVAAALDPRHAYGFVTTHALAS
                     FAVLGAVLLAFTGTESLYADMGHFGKRPIRIGWFVVVLPALVLNYFGQGALLIANPAA
                     VSNPFYHAFPSWALYPMVALATTAAVIASQASITGASSLIRQAVQLGFLPRLNIVQTS
                     ARAYGQIYIPALNWFLLVMVLAAVLGFGTSSNLAQAYGVALTGTMLVTTILTFFVIRY
                     AWRYPLWLCLAATGFFFAIDIAFFGSAMLKVPHGGWFPLAIGVGVFLVMTTWRRGREV
                     MFTRLNESAVPLDPFLKSLMRDPPHRVPGTAIFLTATPEATPHALLHNLNHNKVLHER
                     VVFLTVEIRDVPWVTFEERVTCERVGNGCWRVRVRFGFMNRPDVMRALEVCGALGLEF
                     DMMQTSFFLSRQQVVPGAGGASTMWRWRERMFAAMARNAGNITDYFNIPTNRVIELGT
                     RVEI"
     gene            complement(31774..>32391)
                     /locus_tag="VIG70_08515"
     CDS             complement(31774..>32391)
                     /locus_tag="VIG70_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8250635.1"
                     /translation="NGRQIAEVLARETIDLVVLDLRLPGEDGMDIARKLREESPGLPI
                     VMLTGRKEEADRVMGLELGADDYLTKPFSPRELLARIRALLRRSRAQQTVADGLQKVR
                     AYRFSGWELNVRLRRLTSPKSQVVPLTNTEFNLLAAFLAAPQRVLSRDQLLGLSRLHN
                     DEVYDRSIDTQVARLRKKITSDEGEVIRTERGSGYTFTSAVEIVR"
BASE COUNT         5399 a        10826 c        11126 g         5040 t
ORIGIN      
        1 ggtgccggca accgtgtagc tcatcgccgc gaggcgctcg ccgtcgcgca tccagacgaa
       61 cgcgccgcgg tggcccgagc gctcgtgcgt tatttcttcc agggttgcgg cgccccgggc
      121 gtaggcgacg gcttgttggg ctgcttgggt ttctttttct ctcgattgct gcgctgctcg
      181 cggtttgcca taggaccctc cggggaggcg cccattatcc gccaggcgcc cgccggcgta
      241 ctatggcagg caggtcgtct ccggaggggg aaccatgaaa gtcctgctgc tcgtcgcaca
      301 gctggtcctg gttgccgcag ccgttgctca agaaacggat tatccgagcc ggccgatcaa
      361 gcagatcgtg ccgttcccgc ccggaggcgg ggtcgacatc gtgacgcgca tcgtcggcgc
      421 gaaatggagc gagttcctcg gccagccgat cgtggtcgag aaccgcgccg gcgccggcgg
      481 cacggtgggt gcggacgttg cggcgaaagc cgctggcgac ggctatacgc tgctcacctg
      541 ccaggttgcc tcgcacggcg tcagcccggc gctgtacaac aagctgccgt acgaccacgt
      601 ccgggatttc gcgccgatct cgctcatcgg caagacaccc aacgtgctgg tggtgcaccc
      661 ctcggtgccg gcgcgcacgc tcaaggagtt catcgactac gtaagagcca gcccggggaa
      721 gtacaactac gcctcgcctg gctacggcac ctcgccgcaa atgaccatgg agcttttcaa
      781 gctgaccacc gggctcgaca tgacccatgt gccgtacaag ggcggcgcgc ccgcgctggc
      841 cgacgtgatg ggcgggcagg tcacgggcat gttcggcaac ctgccggagc agctcgccgc
      901 catcaagggc gggaagacgc gcgcactcgc ggtgagcacg ctctcgcgca gcccgcagct
      961 tccggacgtg ccgaccgtcg ccgagtccgg cttccccggg ttcgaggtca ccgtgtggta
     1021 cggcgtgtgc gcgcagtcgt cggtgccgaa acccatcctc gccaagctgc acgcaacgct
     1081 gctgaagacg ctgaacctgc cggagatgaa agcgcggctg gccgaaagca ccatcgacgc
     1141 ttcgcccacc acgccggagg agttcgctgc cttcatccgc gccgagaccg agaaatggac
     1201 ccgcgtggtg cgggacgcca acatcccgaa gcaataaatc aggaaatggg gtctgtcccc
     1261 attttctgga accagcgagc gcgtcccggc cagcgctcga ccaggtcgat gaacagcgga
     1321 tcgatgttct tttcgccctt ggaaatttct ctggccaggg gacctggctc gtcgttgtag
     1381 gcgagcagcg aaaggtcggt catgcgcatc ggatacacga tgccgtcgag gtggtcgcac
     1441 tcgtgctgca gcagcgcggc ccatgagctg gcggcctcgt ggctgtgcgc ccggccgtcg
     1501 aggtcttcgt aagtgatctg gacgtgcatg tggcggggca ccttgccgtg cagtcccggc
     1561 acggagagac agcgctccca taccggcgtc ttcgcgtcgc tcttgggcgt gatcaccggg
     1621 ttcaccatca ccgtccaggg acgcggctgg agccccgagc cgggcgggat gcgcgccgcg
     1681 atgatgcggt agaccacgac gcgcttgccc acgaacacct gcggcgcggc gaggccgctc
     1741 gcgccgatgt cctcgatcgt gtcctgcatg tcggcggcga ggcgccggat ctcgggcgcg
     1801 gtgggatcga cgacttgcgc ggccttctgc agcaggatgg gattgcccat cttggcgatg
     1861 cgaagaaccg ccatcgtcag cctgcgcgcg aggctttagc cccggcaggt ggagggaagg
     1921 aactccgcct tgacgtcggt gccatccacc gtagccccgc agcgccacct tctgaccggc
     1981 gtgccgaggt cttcttcgcg catgatctgg ccggagccgt tgagcggcgc gagcgtgatg
     2041 tcgtggatgg cgaggcgcag gtcgccgatc tcgttgccga gcgtgagccg gatgacgcct
     2101 tcatcggtgg tcctgatgcg ctccacgaag cgcgacggcc tctcgacctc gcagccccag
     2161 ttgtccgcgc cgggaatgtc cgagcccgaa agatagacct cgtggatgat attgcggcag
     2221 ttcgtgagcg cgagaactgc ttccgagatc ttggtgcgcg ccgtgtagct gcgcacctgc
     2281 ggcatgagaa tggacgcgag aatgccgatg accgcggtca ccaccatcac ttcgatcagc
     2341 gtgaaccccg cctgcctgct gctgttgcgg ttccccatgg aagccctgcc ttccgacttt
     2401 tttctttgta gcccgcagct ggctgcaagc ggcatgccaa ccgccggccg ctgctcccaa
     2461 ccttaatact ggccgaaaaa gcgacaaagt gcacattcgg ggatacccct cgtgccgcgg
     2521 cgggtcaggg gctgaccaag aatgtcagcc cttgggccgg gtccaggttt ccgaccaagc
     2581 cagcacgcaa ctcgaggact gccggccgcc gcgctcctgc ggaaacacct tgcccggggc
     2641 ggcctggccg ttgaccgaga gctgcgcgcc gcgcgcgggg atgaaggtgc tgtagacgcc
     2701 gagcgggcgg ttcatcatcc ccggcggcat ggtgagcacg aagggcgcca tgaaatccca
     2761 ccacgtgagg atgatctcgt ccttggacga caccacgcgc tcttcgacgg tgctgagcga
     2821 attgccgctg cgctcgaacc ttgcgtccac gatcggcagc gactcgtcgg cgaacggctt
     2881 gtgcagcagc acttcgatcg tgcgctggag gtagcgcgcg acgccggcgt tgtccgcata
     2941 gatccggttc ttcagcagag agctctcgat gaagagggcg tggccctgtc ccgccggcga
     3001 ccacagcacg cgccagtggc tgacgcgctc ggggatcgtg gtgccgccgt cgttggaaag
     3061 ccgcacgaac gagttctccc cggtcatcac cgggtcgtgc gggtcgacca tgcgaggctc
     3121 ggccatgctc aggaaacgcc ttcgacgatg cgcaggtcgc gatcggccgc gtcgccgagc
     3181 gcgcgcagcg cttcctgata gcccgggctg tcgtgcgcgg cgatcgcctt cgcgaggctc
     3241 tcgaactcgg tgacgacggt gcgctggttc agtccgcctt cgtaggtctt cgacggcgtg
     3301 ccgcgcacga ggaagcggcc gccggcggcc tggatcgccg gcccggcgag cttcgcgtag
     3361 gcggcgagct tgtcctgatt cttgatcgcg cgatagcaga ctacccagta ggctttggcc
     3421 atgaaccctc cgttgattga gattgcctag cttaccgctg aaccgtctat catcgtttgc
     3481 atgacgaccc aggacgcggt ttccctgacc aaggcgctgc ttcgcttcga cacggtcaac
     3541 ccgccggggc gcgagcgcga ctgcgcgcgg tatgccggcg cgatgctgca ggaatggggt
     3601 ttcaaggtcg actaccacga gtacgccgac agccgcacga gcgtgatcgc gcgggcgggc
     3661 ggaagcgacc gcaaggcgcc gctctgcctg accgggcacc tcgacgtcgt gccgctcggc
     3721 gcgcgggcgt ggacccggga ccccttcaac ggcgacaccg acggcgatcg cctcttcggc
     3781 cgcggcgcct ccgacatgaa ggcgggagtc gccgcgatac tcctggcttc gagaacattt
     3841 tcaggaaagc tctcgggcac cccgggagta gtcatcgtgc tgactgccgc ggaagaaggc
     3901 ggttgcgtgg gctccgctca gcttgcgcgc acgcagctcc tgggcaaggc cggcgccatg
     3961 atcgtgggcg agccgacgtc gaactatccg ctcgtcggcc acaagggctc ggtcaagttc
     4021 cacgcgcggt tcaaaggggt gtcggcgcac ggctccatgc ccgagctcgg cgtgaatgcg
     4081 atctacaagg cgggcaaggc gctcgcaagc ctggagggat tcgacttcgc gacgccggaa
     4141 catccggtga tgggaaagcc gacgctgaac gtcggcacga tcgaaggcgg caacacggtc
     4201 aacgcggtgc ccgacgccgc gagtatcggc gtcgatatcc gcaccgtgcc cggcatggat
     4261 cacgaagcgt tgctgagaaa gatacgggct tcgattcccg aggccgagct tgaggtgttt
     4321 tcgaacctga agcccgtgtg gaccgcgccg gagcaggaat ggatccagcg cgtgttcgag
     4381 ctctgcaagc cgtacctgag cgagacgccc gcggcgcgca ctgcgccgta catgaccgac
     4441 gcggcgaacc tgctgaaggt ctacgccggt gcgccgacgg tggtgctcgg gccgggcgag
     4501 gccgcgatgg cgcaccagac cgacgaatac gtgagccagg aacgcatccg ccagtcggtg
     4561 gccatctacg agtccctgat ccgggactgg tgccgcccgt aaattcgggg acggagccct
     4621 aattccggca acgcggagct atcggaatta gggctccgtc cccgaatttc tacatccgcc
     4681 ggatgacgtg gtcctcgctg aggctgacgc gcgtgccgcg gctgaactcg cggcttggag
     4741 tgtcgacgta ctgaaccgct ccgttgtcca tcttgatctc gaggcgctgc agcccctggg
     4801 ccgagctgcc cgcgccgggc aggaccggca cggctaacac cgactgcacg accccgttac
     4861 cggccttgta ggggtggacg ttggtgacga tattgggttg cgccgattgg ggcggggtac
     4921 cgcaagcgcc caaggcggcg gcgacggcga cgagagcgac aggggtaaat cggatcatgg
     4981 caatcctccg tttggaatgg cgcattggaa ctagcaagcg ccgttccggc tcgccccttg
     5041 ctgcacacaa gcaaatgggc tcgtcatccc cgccgtgggt gcgcgctgaa acggtcgaca
     5101 tcgcggtcga ccgccagcgc atcgaaggca ccctggtcgc gccgaccgtg cgcagggacg
     5161 aggtgccggg ggtgctcctg gtgcacggct ggaacggcac cccggccggc tacctcgacg
     5221 aggcgcacgc cctcgcggcg gcgctcggtt gcgtctgcct gaccttcgac ctgcgcgggc
     5281 acggacagtc cgaggcgcag cgcgagaccc tcacgcgcga ggacaacatg cgcgacgtga
     5341 tggccgccta cgacgtcctc gccggccggc agcgggtgca gaagtcctgc atcgcgctcg
     5401 tcggctcgag ctacggcgcg tatctggggg cgctgctgac gtatatgcgg ccggtgcacc
     5461 tgatcgcgct gcgggcgccg gcgctctaca ccgacgacca ctgggagatg cccaagcgca
     5521 agttgcgcgt cgaccgcgaa ttcgccgcct accgccggcg ctcgctgcgc gccgagcaga
     5581 atcgcgcgct cggggcttgc gcccagttcc tcggcgatgc gctggtggtc gagtccgcgc
     5641 gcgacgaagt ggttccgcat gccgtggtcg ccaactacat cgcggcgctc gccaacgcgc
     5701 actcgctcac gtaccgggtg atcgagggcg cggatcacgc gctctcgcag gagccgtggg
     5761 agcgcgccta catcgaattg ctggtgagct ggctgtccga gatgatcagg ggcgccgcgg
     5821 gtcccgctcc cgcgggagcg cgctagggcg ctcgccgggc cgctgcgtct cgccggaggc
     5881 ggcttcgcgt tcctgcttga ggcattcctc gcgcagcgtc cccgagagct cctcgcagcg
     5941 cttgccgggc atcccgccgg tctcgcccgg caggatcgtg ccgcccttga tcgcgccgtc
     6001 gctcggcgct gcgccgtccc ggctctgtcc gggcggaatc gacccacgct cgccgctttg
     6061 gccgagcgcg gcgcccgcga gagccaaggc aaatccgaca acgaatagtc gaaatgcggt
     6121 catggcgaat gctcggagca agatacgctc ccgaatgtga gcaccgtcgg gatggagctc
     6181 cttctcctaa gcagctcgcg cacgccggcg ggctatctga ccgactacct tgccgccatc
     6241 agggctttcg cgggcgggat ccgcgcggcg cacttcgtcc cttatgcggc ggtttcactg
     6301 ccgtgggagg aatactgttc cagcgtaggc aaggcgctcg cagcagcgga cttgcgcgtc
     6361 gagctcgtcg attccgtcga cgatctcatg cgcgccgagc tcatcgtcgt cggcggcggg
     6421 aatactttcc agctcctccg ggagtgccgg cggcggggct tccttccggt catccaggaa
     6481 agggtcaaag ccggcacgcg ctatctcggc tggagcgcgg gggcgaacct ggcgtgcccg
     6541 acgatcaaga ccaccaacga catgccgatc gtggacccgg gctcgctcga cgcgctcggg
     6601 ctggtcgggt tccagatcaa cccgcactac ctcagcgtcg cgctgcccgg ccaccacggc
     6661 gagacgcgcg acgagcgcct cgcggagttc gcgcgcgtaa atccgaagct gccggtcctc
     6721 gggctgcccg agggcgcctc gctgcgtgtg tcggattcca cggtcgagct gctcgggcct
     6781 tatccggccg tacttttcgc cggcgaaaag tccgcgcggc gcctgatgcc gggaaaatta
     6841 ccgacgttgt ccgacgacag cgcgtagcgt ttcaattctt gtggttgcga caaatgacgc
     6901 taggcagacg ggcgcgcggt ggctacgctt gcgccaggtt aggaacacca agaggagcca
     6961 gaaatggaaa aaacagccgt actacgcctc atctccctga ttcccgatcg cgtttcgcgc
     7021 agccagagcg gcagcacctt cgtcggcacg ggcgccgccg ggcgcgccca gcttctcgct
     7081 cccttcctca gctggctcgg ccccctcaac cccatcagca aagaagagca gcagatcctc
     7141 ggcgtgcgtt ccggcggcgg caaggcgctg gtggacggag aagcagcggg cgaggaagtg
     7201 gaggaagcgg cggaccgggt ggagctcgcc gacgaagtcg acgacagccc gatcagacta
     7261 cccgggacgg gaacgcagat tgctgcatag ctgccaccgt taacttgcgg aggcagtcat
     7321 gctgagctgg gccatcggtt tcttcatcct cgcgctcgtc gccgcgttct tcggcttcgg
     7381 cggcatcgca tccagcgccg cgggcatcgc ccaggtgctg ctggtgttgt tcgtgatcgc
     7441 gttcatcgtt tcgctgatcc tcggcttgcg aggccgcggc ggcgtgacgc gtcgagacct
     7501 gttctagcga aacagcgagt cgatctcgcc tcggctgagg ccgatgtcct tcagcgtgcg
     7561 atcgctgagg gagcgtagct ccatccgcga gcgcgccgcc gcccaggcgc tgcaccatct
     7621 tttcaagagc gagatcatgg aaggcactat gcgccttccc ctgcgcccgc ctcaaacggt
     7681 cattgcgcag gcacgtcatg caagactgga atgctagggc gccttgacga acagcgtgac
     7741 gagcgggctc gcgccgcgct gcgcgctcca gtggccgtgc accttggcgt cgaaggccgc
     7801 tcccgcgggc agcgtatcgg cggagaagaa gtgctctccg gccttgaaca cgcgcgagct
     7861 tccgtcctgc aggccgatct ccatttcgcc ggcgagaatg aacacccatt gcggctgcgg
     7921 cgtgcagtgc cattggctgc ggaagccgac cgggctgtgg cgcagctggt agcccgctga
     7981 aggaaacacc cgggacagca tcgcctgcgg cgtgccctcg ggcagcgcga tcgcctcctc
     8041 gcgccatttc gcgcggccat cgcgatcggt gtacagcacg agcttggtga aagtcacgga
     8101 acgagcttac cctttccggc agggagagtg tcctcatgcg catgaaatgg atcgccaccg
     8161 ggttgctggt gctcgccgcg ctggtctacg tcgtctcgga gaaattcgac tggtactacc
     8221 tggccgcctt cagcgaggcg gcgatggtcg gcgcgctcgc cgactggttc gcggtggtgg
     8281 cgctgttccg ccggccgctc aacctgccca tcccgcacac cgcgatcatc ccgcgcaaca
     8341 aggatcgcat cgcccgcggg ctgtcggact tcatcgagca gaacttcctg tcctcggccg
     8401 cggtggtgca gcgcatcgcc gagtttcgcc cggcgcacac gctctgccag tggctcctca
     8461 agcccgacaa cgtggacacg gtcgcaggct atgcgacgcg cttcgtcgcg tatgcgctca
     8521 gcgccgtcga cgacgagcgg gtgcgccgct tcctgcacca gagcatcatc ggcatgtcga
     8581 agaacgtgga cttcgccgct gcggcggccc aggtgctcga cgtgctgacc gagaacaagc
     8641 gccaccacgc gctgctcgac gccgcgctcc ggggcctcga cgacctgctg gcgaaggaag
     8701 agacgcggcg cttcatcgcc gtcgaggtgg cgaagagcgc gccgctcctg aagcgcatca
     8761 gcgactggct gcagctcgac ctcgacgagc gcgccgcgct caagatcgcc gagctcgcga
     8821 tcgccaaggt gcacgaggtc aggcaggacg gcgaccacga gctgcgcagg cgcttcgacg
     8881 acttcgtcgc ggaattcatc ggccggctga agaccgacga gtcgctgcgc aagcgtgtgg
     8941 ccgcgatccg cgacgagctg ctggagagcc ccgcgctggg aagctacatc ggtagcctgt
     9001 ggcaggactt tcgcgcctgg ttgagcgcag acctgggggc aggacagtcc gtcgcgcacg
     9061 ggcgcatcgc cgcgatggtg cgcgcgttcg gcgcgcggct cgaggccgac cgcgagatcc
     9121 ggcagtggat cgacgagcag atcctcaccg cgatcccgcc gctggtcgaa gagcaccgcg
     9181 cgaagatcgg gcgcttcgtc gaggatcaga tcaacggctg gcaggaagag aagctggtgg
     9241 aagagctcga gcgccacatc ggccccgacc tgcagtacat ccggatcaac ggcacgctgg
     9301 tgggcgggct cgcggggctc gcgatcgccg cgctcacgca agtggcgaaa tgagcgcatc
     9361 tttctgtctg gctaggccga cgtcgcgttt ccgctgcctg cgattaaggc cctggcgagg
     9421 ctcgaggcgt atataagaga ccgatgaaga actgcgatat tcaccacgtc aagcacaaga
     9481 actcgttccg gtggaaatgg cggcacacgc ggtccgacgg gacggtgaag gaatcggccg
     9541 agagctataa gctgtactac gagtgcgtga tggcggcgcg cgcgagcggt tacgagcctc
     9601 ggctgaagtg cgcgtgacgc tctagcgctt ttctccgtca ggcctgcggg tccgcttgaa
     9661 catttccagc gtgggccggc acttctccgc gatctccgct gcggtcatgc gcacgtccgg
     9721 cccctcgatc agccagacat gctggccgcc gcgcagcacg agcgctgcgg cgtggcaggc
     9781 atcctgctcg gtgctgaagt ggacgggaac gaggcgttgc agcgtctcgc gatcgaggct
     9841 cttgggcggc ggcgagctgg cgtaataaag cgcgaacggc atcgggagag atgctacgga
     9901 acgtctctcg cgcgcggcgt aagcttgtgt caaaactgcc gcggtctcgg gggctcgtcc
     9961 ttcgccgctt cttcaggctt gcgctcgctt gtcggcttgt ctttttcagg cgcgttcttg
    10021 tcggacggct cgttttcctt ttttccgtcc gactgagcgc cggctcccgc gccggtgccg
    10081 gcggcggcgc tgctaccgcg cacgtcggct ccgccctgga tcttcgtgtt cggatcgatg
    10141 tcgggcgcac gctgttgcgc aacggccggc aaagccagtc cgatgcagat gaaggccacg
    10201 atggcgcgca tggccccgaa attgctgcaa gcggcatgcc atgagaccgt tctggatact
    10261 ggcctttgcg cttgccgtcc tggccgcttg cgatcgtgcg gcgaccgacc gggccgcgaa
    10321 cgaaggcagg gcggcgcagt cggctgccgg cggcagcgcc aacgagggca aggcggcgcg
    10381 cagcgcggcg ggccgctagc gtcggtactt gtcgatcgcg ccgcgcgcga gcgacatgcc
    10441 gtggccgggc cggtccggga tgcggaagcg cccgcccttg cactcgggct gctcctcgaa
    10501 gagatcggac ggcgcccatt ccatgaactc gaccaggaaa ccgttggcca gcgcgcccac
    10561 gacgtgcagc gagatctcgt gcaccacgtg cggcgacacc agcgcgccgt gcgcggcggc
    10621 gagatggccg atcttcagcg tttcgctgaa gccgttggcg cggcacacgt cgggcatgag
    10681 gtacctcgcc gccctgcgct cgagcaagtc gcggaattca aagcgggtga agttgttctc
    10741 tccggtcgcg accggaatgc ggattctctg cgacacctcg gcgcaggagg cgatgtcgtc
    10801 cgccaatacc ggctcctcgt accagacgag gtcgaggtgc tccaactcgg cggcctggcg
    10861 tacgttgccg agcacgtcga ggcgctggtt gacgtcgacc atgaggcgca cgttcggccc
    10921 gagcgcgcgc ttcaccgcct cgacccgcac gcgattcact ttcggatcgg ggtcgtggat
    10981 cttcatcttg tagtacttgc agccgaagcc cgcgaacttc tcggcttcgc gcaccaggtc
    11041 gtcgacgctg tacttcgccc agccgccgct gccgtagcac gggatgcggt ggcgcatcgc
    11101 gccccatacc ttgtagagcg gcttgccgag cgccttgccg acgatgtccc agaggccgat
    11161 gtcgagcgcc gcgagcgcgt acgccgccac gccctgcttt ttcatcccca tgtccacgcg
    11221 gaacatcttt tcccacaggt attcgacgta gagcgggtcc tcgccgatga gcgcgggctt
    11281 gagccgcgct tccaggtagg catgcacggc ctcggcgccg ccgccgccga acaccaggct
    11341 gtagccgagg ccgcggaccc cctggtcggt gctgatctgg gcgacgacga gcctgtgctg
    11401 cggatactgc agcgccatcg gcttcggcgt ggggacggtc aggacgcagg tatcgacggc
    11461 ggtgaccttc atgccgccgc gatgctagcc tacttcttgc gcttttctgc tttcttctct
    11521 tttttcgctt cttcgggggc cttgctttcg cccgcggcgg cgctgaccgg cttgagcacc
    11581 ttgattcggg tgttggcgtc gatccagacg aagccgtcgt cgtcgttcgc ggcgcgagcg
    11641 tcgatcgatg cggcggccag gccgcaaagc agcaaagtca ggagccgagc catggccggg
    11701 cgatgcaagc gacgtgccta taatgaggat cactctggga ggaggcgcat gaacgtccat
    11761 gtccgcatcg catcggctgt cctttttctc gccgcctgtg cccagacgcc gccgggcggc
    11821 ggcgagcttc ccgcattccg ggccgacccc aactggccga ggccgctggc cgaggagaac
    11881 ggcgtgcagc tcgtgcttgg ccaagtcgcc gggatcgccg tcaacccgag taacggccac
    11941 gtctggatcg ttcaccgccc ggcgacgctc ctgccggacg aatggaatgc caaggagagc
    12001 aagccggtca cacatcggtg ctgcaaatcg gccgcgtcgg tgatcgagtt cgacgcggcg
    12061 gggaactacg tgcgcggctg gacgccgagt ggtgccggct tcgactggcc gaaaacggag
    12121 cacggcatcc acatcgattc cgagggcaat gtctggctcg ccggaaacgg caacgaggat
    12181 caccagatcc tcaagttctc gcccgagggc aagttcctcc tgcagatcgg ccgccccggc
    12241 aagtccgagg gcagcaacag ccgcacccag ctcggacgcc cggcgcacat ggtgctctcg
    12301 ggcggcgagc tgttcgtcgc cgacggctac ggcaaccgcc gcgtcgtggt gttcgacgcc
    12361 aagaccggcg cctaccagcg ccactgggga gcgtacggca cggccacgcc cagcgacgac
    12421 aagctgccgt cctacgaccc cgcgcagccg ccgtcgaaga gcttcggcaa cccggtgcat
    12481 tgcgtgcgcg tgtcgaacga cggactggtg tacgtctgcg accggcagaa caaccgcatc
    12541 caggtgttca gccgcacggg cgagtttcgc caggagatcc gcgtcgaggt gcagacgctc
    12601 gccaacggct cgacgtggga catggtgctg tcgcacgacc cggcgcagaa atacctgtac
    12661 gtcgccgacg gcgcgaacgg ccgcatctac atcgtgcgcc gctccgacgg gcagaccgtg
    12721 ggcgcgttcg gccgcaccgg gcgcatggcg ggcgagttca agtggatcca caacatcgcc
    12781 atcgaccgcg acggcaacct gtacacctcc gaggtgggat tcggccgccg cgtgcagaag
    12841 ttcgtgcgca gcggcggcgc gttctgaatc cgctcaagca gaagctggcg cgcggcgagc
    12901 tcgccgcgtc gatgaccgtg cgcctggtct cgagcgtcga gatcgtgcgc atcgcgaaga
    12961 ccgcgggctt cgacgcgatc tatgtcgacc tcgagcactc cgcgttttcc ctcgagacga
    13021 ccggccagct ttcggtgatg gcgctcgagg cgggcgtgcc ggcgctggtg cgcgtgccgg
    13081 cgcgcacgcc cgaatacatc tcacgcgtgc tcgacggcgg cgcgctcggc gtgatcgccc
    13141 cgggcgtcga ctcggcggcg gaggcgaaag aagtcgtcgc cgccgcgaag tacccgccgc
    13201 tcggcagccg cggcctctcg accgggctcg cgcacctcgg cttccgcgcg gtcccggctg
    13261 cggaagcgct gcccgccctg aacgaggcca ccatggtcat cgtgcagctc gagtccgccg
    13321 ccgcgctcgc cgcgatcgac gacatcgtcg cggtcgaggg cgtcgacatg gtgctcatcg
    13381 gcaccaacga cttcctcgcc gacctcggcc accccggcga atatgaccac ccgaaggtgc
    13441 gcgaagccta cgagcgcacc atcgccgcct gccgcaagca cggcaagcac gtgggggtcg
    13501 gcggcctgag ctcgcgcccg gacctggtgc agaagttcgt cgccatgggc gcgcgcttcg
    13561 tttccaccgg caccgacctc ggctttctcc tcgccgcctg caccgagcgc gcgaggcaag
    13621 tgaggaacct gaagatttga aggaggaggt catgaggaaa gggtttttgc ttgccgcgct
    13681 gttgtttgcc gcatccgcat tcggccagta ccccgcaaaa cccgtaaggc tgatcgtgcc
    13741 tctggttccg ggcgggaacc aggacatcgt cgcgcgcgtg gtcgcggagg agctgtcgaa
    13801 gggcctcggc cagcaggtga tcgtggaaaa ccgtccggga cagagcgcga tcatcggcac
    13861 gcagttcgtg aaatccgcgc cggccgacgg ctatacgctg ctctcggtcg cggtcacttt
    13921 cgcgcgcgtg ccggcggtgg tgaaggccgc gggctacgac ccagccgcgg acttcgccgg
    13981 cgtgagcctc gtgtgccgca tcccgcaggt gctggtgctg accccgagcc accccgcgaa
    14041 gaacctgcag gagctgatcg cgctcgccaa agcgaagccg ggcgaagtct ctttcggcag
    14101 ctcgggcaac ggctcgaccg gccatgtcgc ggccgagatg ttcatgcgca tgaccggcac
    14161 ccggctgctg cacgtcacct acaaggggaa cgcgcaggcg atcgtcgacg tgatgggcgg
    14221 gcaggtggcg atgatgttcg accaggtgag cacctccggc agccacgtga agggcggcaa
    14281 gctgcgcgcg ctcggcgtca ccaccaaggc gcgctcggcc gttctgcccg aggtgccgac
    14341 gctgcacgag cagggcctca ccgatttcga ggacgtcacc tggaacggca tcgtcgcgcc
    14401 tgctggaacc ccgcgcgacg ttatttcgaa gcttcacgca accgtggcga gggcgttggc
    14461 aagccccgag ctgcaaaaaa ggtatggcga gcgcggcatc gagatcgtgg cgagccgctc
    14521 gccggaggag ttcagcgcct acgtgaagtc cgaagcggac gcgttcgcaa gactggtgcg
    14581 cgacgccggg atcaaggtcg aatgagcccc gagatcatcc agaccgaggt gttcgccgag
    14641 ctgccggaga agctgcgcaa gagcggctcg ccgcccgagc gcctcgccgc cggcaagagc
    14701 gccaccccga tgggctcgtt cctcgaaggc ccggtattcg accgcgccgg caatctgtac
    14761 gtggtggacc tcgcctgggg ccggatcttc aaggtcaatc caaagggtga attcgagctg
    14821 gtgatcgagt acgacggcga gccgaacggg ctcgccatgc acaaggacgg ccggctcttc
    14881 atcgccgacc acaagaacgg catcctgtac ctgaagcagg gcaagctggt gccggtggtg
    14941 tcgcgctatc accagcaggc gttcaagggc gtgaacgacc tgatgttcga tcccgagggg
    15001 aacctctact tcaccgacca gggcgtgacc gacctcgccg atccgacggg ctgcgtttac
    15061 cgctactccg cggacggcaa gctcgagcag ctcgccaatt gtcttccgag cccgaatgga
    15121 ctggtcgtgg acgggcggat gatctacgtc gcagtgacga gggcaaatgc cgtctggcgc
    15181 ctgccgctta cgcccgacgg caaggtcgtg cggctgggag tgttcctgca tctctccggc
    15241 gggcgcgggc ccgacggcat ggcgatggac gaatccggcg gcctggccgt ggcgcacatc
    15301 gacttcggcg cggtgtggat cttcagccac cgcggcgagg cgctgtaccg cgtcgcgagc
    15361 tgccggagcg acatcgtcac gaacgtcgca tacaaaggta acgagatcta catcaccgac
    15421 tcgggaagcg gctgcatcct gcgcgccagg gttccgaccg ccgggcgagc gttgttctca
    15481 catctatgaa aatcctgata tcgctgttgc tcctgtggct gggaacggcg caggcctttc
    15541 cgaccaaacc cgtaaggatc atcgtggcgt ttccgccggg cggcggcacg gacatcgtgg
    15601 cccggctcct cgcgccgcgc ctcggggacc tgtggggcca gccggtggta gtcgagaacc
    15661 gcgccggcgc gagcggcacg attggaaccg agatcgcggc gcgttccgac cccgacggcc
    15721 acacgctctt tatggcgacg atgggcaaca tgaccgcgaa ccagcacctg tatccgaaga
    15781 tggcggtcga tccgctgcgc gcgttcgcgc ccatcaccaa ggtggtcgac gtgcatttcg
    15841 tcttcatggc gaatcccgcg cttcccgccg acaacgtgag ggacctggtc gaggctgcga
    15901 agaagcgccc gggcgagctc cactacagct cctccgggcc cggcggcgcg ccgcatctcg
    15961 cgatggagct cttcaagcgg caggcgggca tcaatctcac gcacgtgcct tataaaggaa
    16021 gcggaccagg gatgaccgac ctcctcggcg ggcgggtgat gatgacgatg gacagcctgc
    16081 tgcaggggct gccgcagatc aaggcgggca agctcaaggc gctcgccgtc ctcggcccca
    16141 agcgctcgtc gctgctgccg gacgtgccga ccatggccga ggccggcgtc agcggctatg
    16201 cgctcaccaa ctggttcggg ctcctggcgc ccgccgcaac gccgaaagag gtgcttgcga
    16261 aagtacatgc agacgtaacg aaaattcttc gggaaacaga gattcaaaag agaatcgccg
    16321 acatgggtgc cgacgtggtg ggcaactcgc ccgaagagtt cggcgctgcg atgcgcgcgg
    16381 aatcggcgca gtgggcggac gtcatcaagt cggccaacat caaggccgaa tgaaagtcgc
    16441 ggccgagaag gtctgcgaca gccactttca cgtcttcggg cccgccgagc gctatccgta
    16501 tgccggcgac ctgcgttaca cgccgccgca cgcgccgttg aacgaatacc tcaaacttgc
    16561 cagggacctg ggcattgaaa aacatgtctt cgtgcagccc agcgcatacg ggcgcgacaa
    16621 cgcctgcatg ctcgacgcca tgcgcgaggt tggcccggcc aggtgccgtg gaatcgtgga
    16681 tatcgatgag gacgccagcg acgcaacgtt gcgggatctg aatgccgccg gcgtgcgcgg
    16741 cgtgcgcgtc aacgtgaatc ccatcaagcc cgcggaaccg ggctttttca gaagtcttct
    16801 gccgcgaatc gaaaagctgg atgcacgctg cgccgagcgc ggctggatgc tcgatttcct
    16861 cacgccggga tggctgaccc ggcagctttt cccgacgttc gcaaagcttg cgtcgccgtt
    16921 ctcgattgcg cacatgggaa tgtttctcgc gaacggcgga gcagggcaac ccggttttct
    16981 cgagttgctg gatttctgca aaaccaaccc gcgttgctgg gtgaagctga ccggcgtcta
    17041 tcgcgtctcc accgcgccgg gattcgcgga cgcggcaccg ctcgcgcagg cgctgatcgc
    17101 ggcgattccc gaccggctga tctggggcag cgactacccg cacctgtctt tcggccacgt
    17161 ccatacggac gagcttttcg gactactgaa gtcgtgggcg ccagacgcgg ccgcgcagcg
    17221 caggatcctc gaggacaacc cggcgcgcct gttcgggttc tagcgggcac gggctttgca
    17281 ggatcggcgg catgaaaacc acccttctca ccatcgtaat cgccctcgca gtcgcgggtt
    17341 gcgatcgcac ccctgaccag aagccgccgc aagcgggcgc cggagcttcg gctcctcaat
    17401 ccaatacacc gtcgacgccg gcgaatgccg gcactccaag cgcggccgag aagaaggaag
    17461 gcgcgaaccc ggtgcagggc caggtcgatc ctaagcagcc cgagcagcgc aaggacttcc
    17521 agcagaaaag cggcggctag gaaatctctt tccggacgat cgcagcgcct tcggcgagcg
    17581 ccttcagctt gccgaaggcc gcttcgcgcg tgaggtactt catgccgcag tccggcgcga
    17641 gcacgaggcg ctcggccggc acgtgcttga gcgcggcgcg aatgcgcgcg gcgacttcct
    17701 gcggcgtctc gatccgctcg gtgccgaggt cgatcatgcc caccatcagg gtcttgctcc
    17761 caagcgtttt cagcacaccc aggtcgagct tgggctgcgc ggcctcgatc gagacctgcg
    17821 aggcggtgag cgcctcgagt tgcggcagca ccgaatagcc ggtcggcttg tccttcaccg
    17881 ccgcggcgta gccgaagcag acatgcacca cggtggttcc cggaattccc tgcagcgcgc
    17941 ggttgatcgc tttcactcca tagcgcgcgg cgcggtcggg ccgcgcctgc atccacggct
    18001 cgtcgagctg caccacgtcg gcgccggccg ccttcaggtc gcgcagctcc tcgttcaccg
    18061 ccgcggcaaa agcgagcgcg agcgcttcct cgtccttgta gtagtcgtcc tgcgcctgct
    18121 gcgccatggt gaagggcccg ggcagggtaa ttttgatccg ccgcgaagtg ttctttctca
    18181 gaaactccac atcgcgcact tcgaccggcg cgcgccggcg tatcggtccg gcgacgcgcg
    18241 gcaccggaat cgccacgccg ctgcgattga tggtgctgcc gggacgctcg atgtcgatgc
    18301 cttcgagcgt ggtggcgaag cggttcgagt agctctcccg gcgcatctcg ccgtcggtga
    18361 tgatgtcgat gccggcgcgc tccatgtccc ggatcgcgag cagcgtggca tcgtcctgcg
    18421 cttcttcgag aaaggttttt tcaaccttcc atatctcggg catgcggacc cgcgggacct
    18481 tggagccgag ttgggcgcga tcgatcagcc acccgggctg gggatagctt ccgaccacag
    18541 tcgtagtgag gggatattcc acgggagggg ggtagtccag acggactagc atggtacagc
    18601 gacaggcctg ttccttgctc cgttagcctt accccaaccc acggaggccc acagatgaga
    18661 ctttcagctt tggcagttgc cgtttccctg gcgctcggcg ccggaatgct taccggctgc
    18721 gacaggcagg acggcgaccg caccgtttcc aagagccccg gctcgagctc ttcgtccgcc
    18781 ggctcgacga cccccagctc gccgtccagc tcgacttcgg gctcggcgtc ggtcagcacg
    18841 accccgagca ccccgccctc gtccagcgcc tcttcgtccg ccgacgcgac gaagaaggac
    18901 tcggcttcgg gtggcacgag ctccagcagc tcgagcagct ccccgagctc ctcgccgagc
    18961 ggcagcgccg cgagcggcag cagctcgccc agctccgcct cctcgtcgag cgcggcgcag
    19021 gatccggcga aagaagagaa gaagcagtag tagttacaag cgaaagccgt ccttgcccac
    19081 gcgggcaggg cggctttttc tttgaattcg gggacggagc cctaactcca cggctgcgca
    19141 gctttcggag ttagggctcc gtccccgaat tcttgcgcag gcccgcgatc atggagcttt
    19201 cgagcaggtg ctggcgcgcg cgctgtggat cggccgccac ggcgaccagc tcctccatgc
    19261 ggctcctctg cacctgcgga tcgcgctcct cgagcaggcg cttgttgcgg atcgacatcg
    19321 cctgcacctg ctcgatggtc gtggcccggc gctggcgcac gtagacgtcg agcagcgctt
    19381 cgtcggcctc gccgtgccac accctggcga gcttgtcggc caggttcacg gcgtcgtgga
    19441 tgccgctgtt caggccgaag ccgccgagcg ggttgttgag gtgcgcggcg tcgccggcga
    19501 gaaggacgcg cccgaggcgc cattcgctcg ccacgcgctg gtgcacgcgg tagatgctct
    19561 ggtagcggat ttcatagctg gaatcgcggg gcaggaagcc ctgcatccgc ttctgcaccg
    19621 cgccagggtc gagcaacgcg tcgtcgggca ggccgggatc gcaggggaac gcaagccgcc
    19681 acagccccgg gggaccggcg tcgggcatct tgaacaccgc ggcccattcc accggatcgg
    19741 cgacgtaggc gttcagggtg aagccgtgcc tgccgaggtc gtagggcgtg ctgaccacca
    19801 ggaactgctc gggccaggtg aagccctcga actcgaccgg caggagccgg cggatagtgc
    19861 tgcggccgcc gtcggcgccg atcagccagg acgcctcgag cttgcccggg attccctcgg
    19921 actcgaacct caccagcacc gagcgcgcct tgtgctcgaa gccggtgatg cgatgcccga
    19981 agtgcacttc ggcgctcgct tccttcctca ggatgtcgag ctggatcggc gtcaggcgat
    20041 gctgctcgag gtgcaggcga tacgggtaag gggtgacgtc ggcgagcagc ccgaggtcga
    20101 actccgccac caggctgtcg cggcggctgc ggatctgcca gcggcgcacc tgcagcccgt
    20161 gcgcgtgcat ctgatccgta atgccgtagg gcgccatcat ttcctgcgtg ggcggatgga
    20221 acgtgccggc gcgcaggtcg cgcgtgaggc tgggctcgcc ctcgcacacc acgaccggga
    20281 cgccccggcg cgcgagcgcc agggcgcaca tcagccccgt gggaccggca cccgcgatca
    20341 ccacgggaag cttttgcgac atcgctacaa ttctagagtc caaatgagga tctgcatttt
    20401 cggcgcaggc gcggtcggcg ggcatttcgc tgcgaagctc gctgcgagcg ggcacgacgt
    20461 gtcggtcgtc gcgcgcggcg cgcacctgga ggcgatgcgc aacaacggga taacgctcgt
    20521 ccacggcaac gacaccatcc gcgggcgggt gcgtgcggcc gcgagcgcgg ccgagcttgg
    20581 attgcaagat tttgttttcg tcaccctgaa agccaacctg ctcggcgtct ttgcggagca
    20641 agctgcggcg atcgtgggag ccgataccgg cgtggtgttc gcgcagaacg ggattccctg
    20701 gtggtacggg atcgggctcg ccgcaagtga ggcttcaaaa ggcaggccgc ccccgccgga
    20761 tttgtcgagg ttggatcctg gcgggaagct gaaaaaattc ctggagaaga aacaaatcat
    20821 cgggggcgtg gtgtattccg ccaacgaagt gcgcgagcct ggggtgatcg tcaacaatgt
    20881 gcccgggaac aacatgctcg tcgtcgggca ggcggacgat tccgattccc aggcgatcgg
    20941 cgcgctgcgc tcgatcctcg acaaggcgga cctgtattcc ccgcccacga gcgacatccg
    21001 ccaggtcgtc tgggcgaagc tggtgcagaa cctgagcacc gcggcgctgt gcacgctcgc
    21061 cggcgcgacg gtcaaggagg tgcgcgaaga cccgagcctg ggcgagcttt cgaaacggct
    21121 cgccgccgaa gcgagagcca tcgccctcgc gcacggcatc gacgcggcga aggcgccgca
    21181 gcgcccgggc ggcggccagt cgtcgggggc gatcagccac aagccctcgc tcctgcagga
    21241 ctatgagcgc ggccggccga tggagatcga cgcgctgctc attgccgcgc tcgaattcgg
    21301 acgcgcggcg aaggtgccgg cgcccgcgct ggagacggtc gtgccgctcg tcgtgttcaa
    21361 agccgcagcc aaggggttgt acgaccatta aaatgccccg aggcatgcgg ctaaaaacac
    21421 cgatcgaagc gccgtcgctg gaagccgcgc tcgagctcct cgagcgcctg ccggtgccga
    21481 tgtacgtcga gggacgcgat ggccgctgcc tgggcgtcaa cgcggcgtgg gaggagctct
    21541 tcgggatcaa gcgcgagcgc gtcctcggca agggcgtgcg cgagctttgt ccgcagaatc
    21601 ccgagatcgc cgagaagcac gcgatcaagg accgcgagct gtgggcccag cccggctcgc
    21661 agagctacga catcccggtc gtcacgcccg acggccgcag gcgcgagacg gtctactaca
    21721 agtcgacctt tccgccgggc cccgagcccg ccgggctggt gggcgcgatc ttcgacgtca
    21781 cggcgcgcaa gcgcgccgaa attgcgctgc gcgaaagcga ggagcgcttc cgcgcggtgg
    21841 tcgattcggc gaacgaaggc atgctggtct acgaccgccg gctcctcgtg gtctcggtga
    21901 accgcgccgc cgagcgcatc ctgcggctgc cggcttccga gctgatcggc aagcccggct
    21961 tcacctcgct cctgccgtgc atcagcgagg acggcgcgcc gctcggaccg gacgagcgcc
    22021 cgacgctgac gacggtgcac gccgggcagc ctcagaccga ccgcgtggtc ggcgtaaagc
    22081 gctccgacgg gtcggtgacg tggctctcgg tgagcaccac gttcctgcgc cggatggacg
    22141 agaccgagca ctacggcctg gtctccacga tcaccgacat caccgcgcag cacgacgccg
    22201 agcagcgcct gcgcgagagc gagcggcgct tccgcaacac gttcgagctc gccggctccg
    22261 ggatggcgca catcgggatg gaccggcgct tcatccgcgt caaccgccgc ctgtgcgaga
    22321 tcctcggcta tccggaggcg gagctgctcc agctcaccgg ccggcagatc tcgcaccccg
    22381 acgacctcga cgtgatcaac gagcagcgcc cgcgtctgca ctcgggcgag gtcgatgcgg
    22441 tgcggctcga gaagcgctac ctgaggaagg acggctcggt ggtgtgggtg aagttcgcga
    22501 tgacggtgga gcgcgacgcc gagggcaggc cgctgtacga gatcgcggtc tacgacgaca
    22561 tcacggcgca gctcgacgcg cagcttcgcc tgcgcgaaag cgaggcgcgc ttccgtcaga
    22621 ctttcgagct cgccgcctcc ggcatctgcc acgtcatcga cgggcgcttc gtgcgcgtca
    22681 atcgcagcct gtgcgagatc ctcggctatt ccgagaaggc gctggtcggc aagcacgtga
    22741 aggacgtgtc gcatcccgac gaccgcgacg tgaccgacgc cgggcgcgcg cgcgtgcgcc
    22801 ggggcgagat cgaggcggcg cgcttcgaga agcgctacat ccgcggcgac aacggcgcgg
    22861 tggtctgggc cgacatcgcc gtcgcggtgg tgcgcgacgt gttcggcgtg ccgcagtacg
    22921 aggtggcggt gttcgacgac atcaccgagc gcaagcacgc cgaggcggcg gcgcgcgcgg
    22981 cgcaccagga gctcgagcgc tcgaacgccg agctcgagca gttcgcctac gtcgcgtcgc
    23041 acgacctgca ggagccgctg cgcatggtgg cgagctacac ccagctcctg tcgcggcgct
    23101 acgactcgaa gctcgacaag gacgcgcgcg agttcatggc ctacatcgtc gacggcgcga
    23161 cgcgcatgaa gcagctgatc gaggacctgc tcgcgtactc gcgcgtcggc accaagggcg
    23221 cggacttcag gccggtggcc gccgaggcgg cgctgcgccg ggcgctgttc aacctgcgcg
    23281 ccgggatcga ggaggcgggc gcggtggtga cccatgatcc gctgccgacg ctgccggccg
    23341 acgaggtgca gctcgcgcag gtgttccaga acctgatcgg caacgcgctc aagttccgct
    23401 ccgcttccgt tccaagaatc catatcggag tggccgaggg cccacgtgag ttcacttttg
    23461 aagtaaaaga caacggcatc ggcatcgagc cgcagtacta cgagcgcatc ttcatggtgt
    23521 tccagcgcct gcacaacaag ggcgagtacc cgggcaccgg catcggcctc gccatctgca
    23581 agaaggtggt cgagcgccac ggcggccgca tccgggtcga gtcgcgcttg ggcagcggct
    23641 cgagcttcta tttcacgcta cccaaggaaa gaggggaggg ctgatggtcg agaggaacgg
    23701 acccatcgag atcctgctgg tggaggacaa ccccggcgac gtgcgcctca cccgcgaggc
    23761 gctgaaggaa ggcaaggtct acagcaacct ccacacggtc aaggacggcg tggaggcgat
    23821 ggagttcctg cggcgccagg gcaagtacaa ggacgtgccg cgccccgaca tcatcctgct
    23881 cgacctcaac ctgccgcgca aggacggccg cgaggtgctc gaggaaatca agtccgactc
    23941 ggtgctcaag cgcatcccgg tcgtcgtgct caccacctcc aaggccgagg aggacgtgct
    24001 gcgcacctac aacctgcacg ccaactgcta cgtcaccaag cccgtcgacc tcgagaagtt
    24061 catggtggtg gtgaaaacga tcgacgtctt ctggctcacc gtcgtcactc ttcccccgaa
    24121 cggccatagc tgacggactg cacaacgtcc tcctgatcga ggacaacccg ggcgacgcgc
    24181 ggctgatcca ggagatgatc cgcgaggatc cgggcgcgcc tttccaggtg cattgcgccg
    24241 atcgcctgtc gcgcggactc gagcacctcg cgggcggcga gaccgggctg gtgctgctcg
    24301 acctgtcgct gccggactcg atggggctgg agacgttctc ccgggtgtac gcgcactcgc
    24361 ccgccgtgcc gatcatcgtg ctgaccggca acgacgacca gaaaaccgcg ctctcggcgg
    24421 tgaagggcgg cgcgcaggac tacctggtga aggggcgcct ggaccgcgag ctcctgctgc
    24481 gcgcgatgca ctactcgatc gagcgcaagc gctaccaggt ccagctcgag caccaggcga
    24541 actacgatgc cctcaccggt ctgcccaata ggaacctgct ccatgaccga ctgcgccagg
    24601 cggtgtactc gcagcgccag agccgcgcca tcgccgtggt gttcatggac ctcgaccact
    24661 tcaagttcgt gaacgactcg ctcggccact cgaccggcga cctgctgctg aaggcgatgg
    24721 gcgagcgcct gcgcaccgtg ctgcgcgagg gcgacaccgt cgggcgcgtc ggcggcgacg
    24781 agttcgtgct gatcctgaac gaccagtcga acgaggagat catctaccgc gcgatgcagc
    24841 gcatctcggc caaggtgagc gagcccctca tcatcgacgg caaggagctc cacgtcacgt
    24901 gcagcgcggg catcagcctc tacccgcagg acgggcgcga cgtggacacg ctgctgaaga
    24961 acgccgacgc cgcgatgtac cgcgccaagg atcacggccg ctcgaacttc cagttctaca
    25021 cctcggagat gaacgagcgc gtgagcgagc gcctggcgct cgagaacgcg ctgcgccgcg
    25081 cgctcgagcg caacgagctc gcgctgcact accagcagaa ggtgaacctg cagaccggcg
    25141 agatcgtcgg cgccgaggcg ctggtgcgct ggctgcaccc ggaatggggc ctggtgcgcc
    25201 cggcgcgctt catcccgctc gccgaggaga ccgggctgat cgtgagcatc ggcgaatggg
    25261 tgctgcgcga ggcctgccgc cagacccgcg cctggctcga cgctggcctc gacccgggtg
    25321 tggtttcggt gaacctttcg ctgcgccagt tccgccagga gggcctggtg cgcagcgtgt
    25381 cgcgcatcct ggaagagacc gcgctcgatc ccgcgcagct cgagatggag ctcaccgaga
    25441 gcatggtcat gggcaacgtc gacgcagcga tcgcggtgct gcagggcctg aagcagctcg
    25501 gcattacgct ctcggtcgac gacttcggca ccggctactc gagcctgtcc tatctgaagg
    25561 acctgccgat cgacacgctc aagatcgacc gctcgttcgt gcgcgacatc ggcagcggcg
    25621 ccgagctcga cgaaggcgtg ctcgcgcagg ccatcatctc gctcggccac tcgctgcacc
    25681 tgaaagtgat cgccgagggc gtcgagaccg acgcccaggt gcgcttcctg cgccgccacg
    25741 gttgcgacga ggtgcagggc ttcttctacg gcgagccggt ggctccggag gcgcacgcca
    25801 ggctgctgga gaaggcccgg cgccgcgcca ggaactagca aaaagcagga agtaaggcac
    25861 ggaacgcgct attcctacat gggtataatc gcggcccagc atgagcgaac gcgataccgc
    25921 caaggcgcaa ctggtccaca agatcggcga gatcattcgc cacaacggct tcacccaggt
    25981 gcaggcggcg aagatcctcg gcctcacgca gccgaagctg tcgggcatcc tgcgcgggcg
    26041 cttccgcggg gtgtccgagc gcaagctgat cgactgcctg atcagcctcg gccgggacgt
    26101 ggagatcgtg gtgcgggatg cgcccggccg gcgcgccggc ggcaagctca ccgtggtgtt
    26161 tcccgagtga gcgacggcga gaaaaaagcg ggcccggtga tcctcgcggt cgacgactcc
    26221 gaggacatgc tggcgctggt cacaagagcg ctgggcgagg aataccaggt gatgaccgcc
    26281 gccgacccgg ggagcgcgat cgaaaaggcc ttcggcgagc cgcgcccgga cctgatcctg
    26341 ctcgacatcg acatgcccga catcgacggc ttcgaggtgt gccgtgcgct caagggcgag
    26401 ccggccacga gcgcgatccc ggtgatcttc ctcaccggcc gcagcgagcc gcagtcgcag
    26461 gccgaaggcc tggcgctggg cgcggtggac tacatctcca agaccggcac caacgccacg
    26521 atcctgaaga gccgcgtcgg catgcacctc gcgctcgtca accagcgcgc cgagctcgag
    26581 cgcctggtgc tggagcgcac cgagcagctc gagcgcacgc gcgtagagat gatcaagcgc
    26641 ctggcacgcg tactcgaggt ccacgagagc caggcggtcg gcaaccgcgt gatgcgcctc
    26701 gcgcactacg ccaagttgat cgcgcagggc tcgggcgccc tgcctgacat gacggagatg
    26761 atccagaagg cggctccgct gcacgacatc ggcaagctcg gcgtgccggc cgagatcctg
    26821 cgcaagcgcg aaaagctctc ggcgccggac tgggagcgca tcaagcgcca tcccgagatc
    26881 ggcgccgaga tcatcggcga gcacaccgat ccgctgctca ggctcgcgcg ccagctcgcg
    26941 ctcacgcacc acgaaaatta cgacggcagc ggctatccga aggggctcaa gggcgaagtc
    27001 atcccctggg gcggacgcgt gatggcgatc gtcgactcgt tcgaggcgat gaccagcacc
    27061 cagttctacc gcgaagcgct gcccatcgag cgcgcgagcg gcgagatcga gcgcggcgcc
    27121 ggaagcaaat acgatccgaa gctggtcgag gcgttcaaga aggcgctgcc ggtgatgcag
    27181 aaggtgcgcg agacctactc cgaccagctc ggcgacctga tcaacctcga tttcgcgccc
    27241 aaggttgccg ccaagccagc acctcccgcc ccggcagcca ggccggcggc gccgccgccc
    27301 gcggcgaaac ccaagctgag cgcggcagag atggtaaaag cagcagcaag gaagaaataa
    27361 cgccccgaaa tgagcaagat attcgacaag cttaaaggcg ccgagcgcga gcgcatcgag
    27421 cgcgagaaga gcggcgagga agtggcgcag gcgcgcgccg gcgaggagcg tcgcgggctg
    27481 gatctcgcgc gcgagcgcca ggccgccgag gtggagctgc gccgcctcgc gcattcgcgc
    27541 gccgaggccg aggccaaggc ggcgcgcctg gtgaccgagc gcgagcgcgc cgaagcggcg
    27601 gccgcggccg aagcgcgcca gcgcgaggaa gccgagaaga aagcggcggc gatcgccgag
    27661 ggccgcaaga agaaggagga agaggcggcc cagcatgcaa aggcgcgcgc cgagtccgag
    27721 gcgcaggccg cggcggcgat gaagcagcgc gccgccaacg aagagcaggc gcggcgcgcg
    27781 gcgcgcgagc gtgcgcacgg cgaggcgaag gcgctcgagg ccgcgcgcga gcgcaagacc
    27841 gctgaagaca agcagaagcg cgaagccgag acgcgagtcg ccgccgagct ggaagcgggg
    27901 cgcctggcgc aggagcgcgt cgcgaccgaa cgcgcggcgt ccgagcaggc gcaagggcgg
    27961 atcgcggcag agacgcgcgc cagcgcgctc gccgaggagc gccggcgcgt ggaaaccgcc
    28021 gcggccgagc ttgcccgcgc gcgcgccgag gcggaggaac aggcgcgcgt cgcggcggag
    28081 gaaaggatcg ccgcggagcg caacgccacg gcggcgttgc gcgagcgcga agcgcaggac
    28141 gcacatgccg cgaagcacgc gcaggcgcgc gcggacgccg aggccgcggc cctgcaatcc
    28201 gccgcggagc gcaaggaggc ggaagaacgt gctcttgaag aggcaaaagc aaaaatagaa
    28261 gcggccaatc gcgccaccca gctcgcggag gagcggcgcg cgctcgagca ggcggccgac
    28321 gccaaggcgc gcaagcgcgc cgaggtcgag acgcaggcgc tggaggcggc caagcgccag
    28381 gcgagcgcgg aggacgccgc gcgccaggcg gcagagacgc gcgcccacgt cgagaccgaa
    28441 gccgcgcgcc ttgcgcgcga gcgcgagatc gccgacggcg agctcaagcg tatcgcccac
    28501 gagcgcctcg ccgccgaagc cgacgccgcg caggcggcgc gcgaagccga agaggccaag
    28561 gcgcgcgcgg ccgcggaggc gggcgagcgg acagccgcag agcaggcgct cgccgccgca
    28621 gcgctcgagc gccatgaagc cgacgcggcc ctgagcgagc gctcgaaggc gctcctgagc
    28681 gcggaaagca tcgcgctcca ggcggcgcgc gagcgcgagc aggcggagac cgcggcgcgc
    28741 aaggctgcag aagatcgcgc gcaggcggaa gttgacgccg cgcgcgtcgc gcgcgagcgc
    28801 gagaaggcag aatcgcagac cgaggcggcg ctcgccgcgc gcatcgaggc cgaggaccga
    28861 cggcgcaggg cggcggagga gctcgcggcg gccgagggcg ccgcggcggc cgagctgcag
    28921 gggcgcatcg ccgccgagcg cgcgctggag gccgaagcgc gccgggcggg cgatgccgaa
    28981 aagcaggcag tcgatgcagc cgcccagcgc gcggcgctgg atgcggagct gcgccgcggg
    29041 gcgcaggcgc gggccgaggc ggacgcggca gcggcacgcc gcgcggcgga gcgcgagcac
    29101 gccgaagcga aagcggcgtc cgaccatgaa atgcgcctcg aagcggagcg caagagcgag
    29161 cggatctcgc tcgagaaagc cgaggcggag tcgcgcgcga cggccacgct ccaggcgcgc
    29221 atcgccgccg aggaggcgct cacccgcaag caggtggaag ccgaggcggc ggcgcggcag
    29281 gccgaggaag cggcgcgcgc aagagccgag cacgaagaag agcttgcgct tgccctcgcc
    29341 gcgcgcgcgg cggccgatgc cgccgcggcg aaggccaagg cggtgcagct gcgcgccgac
    29401 gagaaggcgc tcgccgctct gcaaaaaaaa ttggacgcag aaaagaagct cgagctcgcc
    29461 gcgggccggc gcgccgaagt ggaatccgcg gcggccgacc tgctgcgcgc gggcatcgcc
    29521 gccgaggaag cgctggaaca gcagacgcac cgcgccgcgg acgcatccgc tcgcgtcgtt
    29581 tcaacgaaag aagagcggat cgcgctcaag gcgcggctcg cgcaactgcg gccgcggcgg
    29641 ctgccgctcg tggccgcagg cctcgcgatc ggggttgccc tgctggcggc cttcgtgttc
    29701 gtcgtgacgc aggagccgcc cgtgccggcc tcgttccagc cggtcggcga gccgctcaag
    29761 ctgaagctcg aatttgcttt tccggcggcg cgacggtagc ttgcgcgcct gcttcccaag
    29821 caggttctag cgacactttt ggagagagac cgctccggca aggataccca gactgcgctg
    29881 acgctcgccg cgctcggcgt cgtcttcggc gacatcggca ccagcccgct gtacgccgtc
    29941 aaggagacct tctccccggc gcacggcctg ccgttcaccc cggccaccat cctcggcggc
    30001 ctctccacca tcttctggtc gctgatcatc gtggtgtcgc tcaagtacgt ggtgctggtg
    30061 atgcgcgccg acaaccgcgg cgagggcggc acgatggcgc tgctcgcgct cgccagcaac
    30121 gcgctcaagc agcggccccg gcagcggctg gcggtgaccc tgctcggcct gctgggcgcg
    30181 tcgctgtttc tcggcgagac cgtgatgacg ccggcgacct cggtggtgtc ggcgatcgag
    30241 ggcctgcagg tggggacgcc gctcttcgag cgctgggtgg tgccgctctc tgtgctggtg
    30301 ctgttcggcc tgttcgcgct gcagcgcctc ggcacggcga aggtgggcgc gctgttcggg
    30361 ccggtgtgcc tgctctggtt cctggcgctc ggcgcggtcg gcctctggaa catctggctc
    30421 aaccccggcg tggcggcggc gctcgacccg cgccacgcct acggcttcgt caccacccac
    30481 gcgctcgcct cgttcgcggt gctcggcgcg gtgctgctcg cgttcaccgg caccgagtcg
    30541 ctgtacgcgg acatgggcca cttcggcaag cgcccgatcc gcatcggctg gttcgtggtg
    30601 gtgctgcctg cgctggtgct gaactacttc gggcagggcg cgctgctgat cgccaacccg
    30661 gcggcggttt cgaacccgtt ctaccacgcg tttccgagct gggcgctcta cccgatggtg
    30721 gcgctcgcca cgacggccgc agtgatcgca tcgcaggcct cgatcaccgg cgcgtcctcg
    30781 ctcatccgcc aggcggtgca gctcggcttc ctgccgcggc tcaacatcgt gcagacttcg
    30841 gcgcgcgcct acggccagat ctacatcccg gccctcaact ggttcctgct ggtgatggtg
    30901 ctcgccgcgg tgctcggctt cggcacgtcg agcaacctcg cccaggccta cggcgtcgcg
    30961 ctgaccggca cgatgctggt caccaccatc ctcacgttct tcgtgatccg ctacgcctgg
    31021 cgctatccgc tctggctgtg cctcgccgcc accggcttct tcttcgccat cgacatcgcg
    31081 ttcttcggct cggcgatgct caaggtgccc cacggcggct ggttcccgct cgcgatcggc
    31141 gtcggcgtgt tcctggtgat gacgacctgg cggcgcggcc gcgaggtcat gttcacgcgg
    31201 ctgaacgagt cggcggtgcc gctcgacccg ttcctgaagt cgctgatgcg cgacccgccg
    31261 caccgcgtgc cgggcaccgc gatcttcctc accgcgacgc ccgaggcgac gccgcacgcg
    31321 ctgctgcaca acctcaacca caacaaggtg ctgcacgagc gcgtggtgtt cctcacggtc
    31381 gagatccgcg acgtgccgtg ggtgacgttc gaggagcgcg tcacctgcga gcgcgtgggc
    31441 aacggctgct ggcgggtgcg cgtgcgcttc ggcttcatga accggcccga cgtgatgcgc
    31501 gcgctcgagg tgtgcggcgc gctcggcctg gagttcgaca tgatgcagac ctcgttcttc
    31561 ctgtcgcgcc agcaggtcgt ccccggcgcg ggcggcgcgt cgaccatgtg gcgctggcgc
    31621 gagcgcatgt tcgccgcgat ggcgcgcaac gccggcaaca tcaccgacta cttcaacatt
    31681 cccaccaacc gggtgatcga gttggggacg cgggtggaaa tctagctgca gccgtacccc
    31741 caagcctagg cgggcgagcc tcccgtgaga gggctagcgg acaatttcta cggcggaggt
    31801 aaaggtgtaa cccgaaccgc gttccgtgcg gatgacttca ccttcatctg atgtgatctt
    31861 tttacgcagc cgcgcaacct gcgtgtcaat cgagcggtcg taaacttcgt cgttgtgcag
    31921 gcgcgagagg ccgagcagtt ggtcgcgcga gagcacgcgt tgcggcgcag cgaggaatgc
    31981 tgcgagcagg ttgaactccg tgttagtcag tgggacgacc tggcttttcg gcgaagtgag
    32041 gcggcgcaga cgtacattca gctcccagcc agagaagcgg taggcgcgta ccttctgcag
    32101 gccgtcggcc accgtctgct gggctcggct gcggcgcagg agcgcccgga tgcgcgccag
    32161 gagttcccga ggggagaacg gcttggtaag gtaatcgtcg gcgccaagtt cgagccccat
    32221 cacgcggtca gcctcttcct tgcgtccggt cagcatgacg ataggaagtc ccggcgactc
    32281 ctcgcgcagc ttccgcgcga tgtccatgcc gtcttcacca ggcagacgca ggtctagcac
    32341 cacgagatcg atcgtttctc gtgctagcac ctccgcgatc tgccgtccgt t
//
LOCUS       DATBSZ010000131         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_496689, whole genome shotgun sequence.
ACCESSION   DATBSZ010000131 DATBSZ010000000
VERSION     DATBSZ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496689"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VIG70_08520"
     CDS             <1..474
                     /locus_tag="VIG70_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310601.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY8250636.1"
                     /translation="HGQPGEICIRGPQVMAGYWQRPDETAKVLGKDGFLHTGDIGIMD
                     DKGFIRIVDRKKDMILVSGFNVYPNEIEQVLAMHPGVLECAAIGVPDEHSGEVPKVFV
                     VKKDPQLTEEDVLEHCKKGLTGYKRPKYVEFRSELPKTNVGKILRRALREEKKAA"
     gene            482..1399
                     /locus_tag="VIG70_08525"
     CDS             482..1399
                     /locus_tag="VIG70_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8250637.1"
                     /translation="MTRANLVAGLAWIGLAILCWTPLFSVAKRTLPYLDAYALGSVRY
                     LFGVALLVLLLVLFEGRRALRYEERLGAATLCGLIGITGFNLFVWIGLGYTRPEHASI
                     ILALQTPLTALAVWLAHGQRPAPFTFGCVIAAIGGVVLVITKGDLSNALAGGSLLGDT
                     LIFLGAVSWVTYTLSASRFSGWSPLRFTVLTCIPGGIGLIVANGVALAIGAAAVPSLE
                     TLFTAGWQILYFSVGTVVLGVLGFNAAARRLGPLNTMLMLNLIPIGVFGIEAALGRSF
                     AAIELAGAAVVIGALVANNLYLRGVSTSR"
     gene            complement(1369..>3167)
                     /locus_tag="VIG70_08530"
     CDS             complement(1369..>3167)
                     /locus_tag="VIG70_08530"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY8250638.1"
                     /translation="LPLERIQEFLAPYPGDAYPRLPDLKTLYSGMEKTPAPNPVFSEG
                     IRGQGRNSVRTWCNANYDPDPEFLRGSNSWAVAGARSASGKPLLAADPHLGLTAPPVW
                     YFAHLHAPGLDAIGGTLPGVPAILVGRNDRIAWGLTNTGADVQDLYLEKLDAPFTQTE
                     ETIKVKDAAPERLVVRRSRHGPVISDVVRSALEASPRGYALALAWTALAEDDLTMQAG
                     FKIARARNWNEFLAAARTLHAPPQSASYADANGNIGFIAAGRVPVRKRANDLHGLAPA
                     PGWDARYDWTGYVPFDALPRAFNPPSGSVILANHKIVPRGYRHHITYEWQPPYRARRI
                     EELLGSSIHDVASFKRMHADVVSIAVRELLPLFVAANPDHEVMKKLAAWDATMAAERA
                     EPLIMAAWWREFARALYADDLGDAFRAGWSARPVFIGNVLSSQSHWCDNVRTPPAESC
                     KSVLVDSLESALEDLKARYGNDWKWGEAHVARHRHRPFTREPWLARFFDIRVPSAGDA
                     YTVNAGAVDFNSDSEPFANRHAPSLRAISDLADPQASVFIHSGGQSGNPLSAQYRNFA
                     AAWARGEYVPMVTERRRLEADGVQRLVLTPRK"
BASE COUNT          473 a         1032 c         1114 g          548 t
ORIGIN      
        1 cacgggcagc cgggcgagat ctgcatccgc ggcccgcagg tgatggcggg ctactggcag
       61 cggcccgacg agaccgccaa ggtgctcggc aaggacggtt tcctgcacac cggcgacatc
      121 ggcatcatgg acgacaaggg atttatcagg atcgtggacc gcaagaagga catgatcctg
      181 gtgtcgggct ttaacgtgta cccgaacgag atcgagcagg tgctggccat gcacccgggc
      241 gtgctcgagt gcgcggcgat cggcgtgccc gacgagcatt ccggcgaggt gccgaaggtg
      301 ttcgtggtga agaaggaccc gcagctcacc gaggaggacg tgctcgagca ctgcaagaag
      361 ggactgacgg gctacaagcg cccgaaatac gtcgaattcc gctccgagct gccgaagacc
      421 aacgtcggca agatcctgcg ccgcgcgctg cgcgaggaaa aaaaggcggc ctagggagcg
      481 cttgacccgc gccaatctcg tcgccgggct ggcgtggatc ggcctggcga tcctgtgctg
      541 gacgccgctg ttctcggtag cgaagcgcac gctgccttac ctggacgcct acgcgctcgg
      601 ctcggtgcgt tatctcttcg gcgtcgcgct tctcgtgctg ctgctcgtct tgttcgaggg
      661 tcgacgggcc ctgcgctacg aggagcgcct cggcgctgcg accctttgcg ggctgatcgg
      721 cattaccggc ttcaatctct ttgtgtggat cggccttggt tacacgcggc ccgagcacgc
      781 ctcgatcatc ctcgccttgc agacgccgct cacggcgttg gcggtgtggc tggcgcacgg
      841 ccagcgcccg gcgcccttca cgttcggttg cgtcattgcg gccatcggcg gcgtggtgct
      901 ggtgatcacc aagggcgatc tctccaacgc gctggccggc ggcagcctgc tcggcgatac
      961 gctgatcttc ctgggggcgg tgtcctgggt gacttacacg ctttccgcga gccgcttctc
     1021 gggctggtcg ccgctgcgct tcaccgttct gacctgcatc cccggaggta tcggcctgat
     1081 cgtcgccaac ggcgtcgcgc tggcgatcgg cgccgccgcc gtgccgagcc tggaaacctt
     1141 gttcaccgcc ggttggcaga ttctctactt ctcggtgggc accgtagtgc tcggcgtact
     1201 cggattcaac gccgcggcgc ggcgcctcgg tccgctcaat accatgctca tgttgaatct
     1261 catcccgatt ggcgtcttcg gcattgaggc cgcgctcggc cgcagcttcg ccgcgatcga
     1321 gctcgccggc gcggcggtcg taatcggcgc gctggtggcg aacaacctct acttgcgtgg
     1381 ggtgagcact agccgctgaa cgccgtcggc ctcgagccgg cggcgctctg tgaccatcgg
     1441 cacgtactcg ccgcgcgccc aggcggcggc gaaattgcgg tactgcgccg agagcgggtt
     1501 gcccgactgg ccgccggaat ggatgaacac cgacgcctgc ggatcggcga ggtcgctgat
     1561 cgcgcgcagg ctgggcgcgt gccggttggc gaacggctcg gagtcgctgt tgaagtcgac
     1621 ggcgccggcg ttcacggtat aggcgtcccc tgcgctcggc acgcggatgt cgaagaagcg
     1681 cgcgagccag ggctcgcgcg tgaacggccg atggcggtgg cgcgcgacgt gcgcttcgcc
     1741 ccatttccag tcgttgccgt agcgtgcctt gagatcctcc agcgcgcttt ccagcgagtc
     1801 gaccagcacg ctcttgcagc tctccgccgg cggggtgcga acgttgtcgc accagtgcga
     1861 ttggctcgac agcacgttgc cgatgaacac cgggcgcgcg ctccagccgg cgcggaaggc
     1921 gtcgccgagg tcgtcggcgt agagagcgcg cgcgaactcg cgccaccacg cggccatgat
     1981 gaggggctcg gcgcgctcgg ccgccatggt cgcgtcccag gcggcaagtt tcttcatgac
     2041 ttcatggtct ggattcgcgg cgacgaagag gggcagcagc tcgcgcaccg cgatggagac
     2101 gacgtcggcg tgcattcgtt tgaagctggc aacgtcgtgg attgaagaac ccagcagttc
     2161 ctcaatgcgc cgcgcccggt acggcggctg ccactcgtag gtgatgtggt gccgataccc
     2221 ccgcgggacg atcttgtggt tggcgaggat tacgctgccg ctcggaggat tgaacgcccg
     2281 cggcagcgcg tcgaagggca cgtatcccgt ccagtcgtag cgcgcgtccc agcccggcgc
     2341 aggcgcgagg ccgtgcaagt cgttcgcgcg cttgcgcacg gggacgcggc cggcggcgat
     2401 gaagccgatg ttgccgttgg cgtcggcata gctcgcgctc tgcggcggag cgtgcagcgt
     2461 gcgcgcggcc gccaggaact cgttccaatt acgcgcgcgg gcgatcttga agccggcctg
     2521 catcgtcagg tcgtcctcgg cgagcgcggt ccaggcgagg gcgagcgcat agccccgcgg
     2581 cgaagcctcc agcgcgctgc gcacgacatc cgagatcacc ggcccgtggc gcgagcgccg
     2641 cacgaccagg cgctcgggcg ctgcgtcctt caccttgatt gtctcctcgg tctgcgtgaa
     2701 cggcgcatcg agcttctcga gatacaggtc ctgcacgtcg gcgccggtat tggtgagccc
     2761 ccaggcgata cggtcgttgc ggccgacgag gatcgccggc acgccgggca gcgtgccgcc
     2821 gatcgcatcc agtccaggcg cgtggaggtg cgcgaagtac catacgggcg gcgcggtgag
     2881 tccgaggtgc ggatcggcgg cgagcagcgg cttgccgctc gcgctgcgcg caccggcgac
     2941 cgcccagctg ttcgagccgc gaaggaattc ggggtcaggg tcgtaattcg cgttgcacca
     3001 agtgcgaaca gaattacgac cctgaccccg aattccttcc gaaaacaccg gattgggcgc
     3061 gggcgtcttc tccattcccg aatacagcgt tttcaggtcc gggaggcgcg ggtacgcgtc
     3121 gcccggatac ggcgcgagga attcctggat gcgctccagc ggcagcg
//
LOCUS       DATBSZ010000132         5813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2273068, whole genome shotgun sequence.
ACCESSION   DATBSZ010000132 DATBSZ010000000
VERSION     DATBSZ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2273068"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VIG70_08535"
     CDS             complement(<1..674)
                     /locus_tag="VIG70_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8250639.1"
                     /translation="MGGLVRILEGRFGRLVLSDLAPGGACTAQADPAILMQPGDAELV
                     FLNPGESYTATAATRVLVLHAASEWLRTSFPAIFGPAEQPPFAQPREPITPRIRRLAD
                     TLAVEVLNDRFVSAERLDFLLQELLLSIVETYLARRRTASPAWRGSRFEDSRIRRAIA
                     LLRARPNKELNMDELASQVGLSRSRFYDLFQLCTGFSPRAYLDMLCVETAISRLSSGQ
                     GKIAEVS"
     gene            complement(705..1175)
                     /locus_tag="VIG70_08540"
     CDS             complement(705..1175)
                     /locus_tag="VIG70_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY8250640.1"
                     /translation="MDDINTLRRILRESKVIAVVGLSADWYRPSFFAAKYMQEHGYRV
                     IPVNPKYDSVLGEKCYRSLREVPERVDIVDIFRKSADVMPIAEDAIAIGAKVLWQQLG
                     VRNEAAAAKARAAGLEAVLDRCVKIEHGRLFGGLNWVGVNTRVISAKRPRWLAY"
     gene            1220..1561
                     /locus_tag="VIG70_08545"
     CDS             1220..1561
                     /locus_tag="VIG70_08545"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEY8250641.1"
                     /translation="MYVEERVYRLKIGAVAEYCKTYEELGMKVQLKHLPHMVGYYVTE
                     VGGLNTMVHLWAYDSLDQRDKCRAAMQADPEWQKYLAKSRPLMETQETRIMKCAPFFL
                     ERLKKMLAAAK"
     gene            1588..2481
                     /locus_tag="VIG70_08550"
     CDS             1588..2481
                     /locus_tag="VIG70_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8250642.1"
                     /translation="MLLEAAARSPLPTLDKVSAFVARRLYSDRCKTVAPKKVPETQNR
                     LLLTPGGVPVRSYRPAGIAKTDVLPALVFFHGGGWTIGDLDTHDVLCRQLASGARCEV
                     FSVEYRLAPEAPFPAAVDDCIAATEYVSSITKSNRIAVGGDSAGGNLAAVVALHLKQL
                     LCFQLLIYPATGQNMESESLRRNGEGYLLTLPMMERFRSNYLPRPADYHDWRASPALA
                     SSHAGLPPAFILTAGFDPLLDEGRQYAELLARAGVEVAYREYPDMIHGFILMGGALDT
                     ANEAVAECCVQLRKAFEKVTA"
     gene            2478..4160
                     /locus_tag="VIG70_08555"
     CDS             2478..4160
                     /locus_tag="VIG70_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY8250643.1"
                     /translation="MKSDLKTRHGGRILADALAAQGVKLAFGVPGESYLPVLDGLHDL
                     QDRLRFVICRQEGGASYMAKAYGKLTGEPGVLFVTRGPGATNGAIGVHTGLQDSTPMV
                     VFIGQVPNEFAEREAFQEIDYRRMYGQMAKWVGQIDRVERIPEYVSRAFHTAAAGRPG
                     PVILALPEDALFSEAAVADVPKHHVVRPAPSPLQVNELKKLLERAKTPFVLLGGGGWD
                     RSTTQKLQKWIEANGLQAGTSFRCQDLFDNRSPSYAGDVGIGINPKLAQRVKEADVLL
                     VIGARLGEMTTSAYTLVEAPVPQQTLVHVHAGAEELGRVYRPALAINSGMAQFVDAVQ
                     GLSFKNPAWKAQAEEARKQFLEWTEPRPMPGKVQYGEIIRWLSDRLPEDTIIAGGAGN
                     FAGWLHRHFRYKGFRTQLGSTNGSMGYGYPAAVAAKLAQPQRTVFALCGDGDFLMTGQ
                     EIATAVQYGANFVAVVVNNGLYGTIRMHQEREYPGRVYGTELRNPDFAAYARAFGGHG
                     ETVERTADFAPAYERAAASGKPAIVELKIDPDAITPATTLSAIREKALKSKQ"
     gene            complement(4144..4536)
                     /locus_tag="VIG70_08560"
     CDS             complement(4144..4536)
                     /locus_tag="VIG70_08560"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY8250644.1"
                     /translation="MKNGKAFPIPVRAQYSCSKCPAYCCSYPEIEVTPRDIERLAKHF
                     DLPYREAEERFTKYDAKEKVRELRHRQDRIFKTVCTFLDQKTRRCTVYEARPGVCRNF
                     PDSPRCGYYEFLKFERAQQDDPKFIALT"
     gene            4648..5370
                     /locus_tag="VIG70_08565"
     CDS             4648..5370
                     /locus_tag="VIG70_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250645.1"
                     /translation="MKTLRMVVAAATMMTGTHALAHGRLVDLSVYDRSEGRWLQVYRH
                     EGRAYVVGKPGHEYRVRVANRLGEDLLAVVSVDGINVITGQTADPSQSGYVVAPRGVL
                     DMNGWRRSLAQTAAFYFTALPDSYAARTGRPDDVGVIGVALFRKKSQPIARSAPEVAA
                     SGPRAAQERDASRLGTGHGRHEHSPAVHVTFERATSSPAEVIVLHYDSHANLVARGVI
                     REPVQVAPTPRPFPGFVPDPQG"
     gene            5382..>5813
                     /locus_tag="VIG70_08570"
     CDS             5382..>5813
                     /locus_tag="VIG70_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8250646.1"
                     /translation="MEAPDEELMLAYGRGDAGAFETLYKRHRGPVYRFVLRAIKNRST
                     AEELFQEVWIRVIEARSRYTPQARFTTWLYTIAHNLLVDHWRRKGLTLVQLDEEQLVA
                     APDNPARQAEAREALTRLLQAIESLPAAQREAFLLHEEAGLS"
BASE COUNT         1011 a         1929 c         1918 g          955 t
ORIGIN      
        1 gacacctcgg cgatcttgcc ctgcccggag gacaggcgcg agatcgcggt ctcgacgcac
       61 agcatgtcga ggtaggcgcg cggcgagaag ccggtgcaga gctggaacag gtcgtagaag
      121 cgcgagcgcg agagcccgac ctggctcgcg agctcgtcca tgttgagctc cttgttcggc
      181 cgcgcgcgga gcagcgcgat cgcgcgtcgg atgcggctgt cctcgaagcg cgagccgcgc
      241 cacgcgggcg acgccgtgcg gcgccgggcg aggtaggtct cgacgatcga gagaagcagc
      301 tcctggagca ggaagtcgag cctctcggcg gagacgaagc ggtcgttcag cacttccacc
      361 gccagggtat cggcgagccg gcggatgcgc ggcgtgatcg gctcgcgcgg ctgcgcaaac
      421 gggggttgct ccgccggacc gaagatcgcc gggaagcttg tgcgcagcca ctcgctcgcg
      481 gcatgcagca ccagcacccg cgtggcggcg gtggcggtat agctttcgcc ggggttgaga
      541 aaaaccagct cggcgtcccc gggctgcatc aggatcgccg gatcggcctg ggccgtgcag
      601 gccccgcccg gtgccagatc gctcagcacg agccggccga atctaccctc gaggatgcgc
      661 accaaccccc ccatgccgga gaggatacct tacccggagg gggtttagta ggccagccag
      721 cgcggacgct tggcggaaat cacccgggta ttgaccccaa cccagttcag gccgccgaac
      781 aggcggccat gctcgatctt gacgcagcgg tcgagcaccg cctccagccc ggcggcgcgg
      841 gcttttgccg ccgcggcctc gttccgcacc ccgagctgct gccagaggac cttggcgccg
      901 atggcgattg cgtcttcagc gatcggcata acgtccgcgc tctttcggaa tatgtcgacg
      961 atgtcgacgc gctccggaac ctcgcgcagc gagcgatagc acttctcacc aagcaccgag
     1021 tcgtacttcg ggttcaccgg gatcacgcgg tagccgtgct cctgcatgta cttggcggcg
     1081 aagaagctcg gccggtacca gtcggccgac aggccgacga ccgcgatcac tttgctttcc
     1141 ctgaggatgc ggcgcagcgt gttgatgtcg tccatgcctc gaagtctata atcaaatcat
     1201 cccggaggag atcgatcgca tgtacgtgga agagcgcgtg tatcggctga agatcggcgc
     1261 cgtcgccgag tactgcaaga cctatgaaga gctcgggatg aaggtgcagt tgaagcacct
     1321 gccgcacatg gtgggctact acgtgaccga ggtcggcggc ctgaacacca tggtccacct
     1381 gtgggcgtac gacagcctcg accagcgcga caagtgccgc gcggcgatgc aggccgaccc
     1441 cgaatggcag aagtacctcg ccaagtcgcg cccgctgatg gagacgcagg agacgcgcat
     1501 catgaaatgc gcgccgtttt tcctcgaacg tcttaaaaag atgctcgccg ccgcaaagta
     1561 gcaatgaaag ttcaccctca ggtcgccgtg ctgctcgagg cggcggcgcg ctcgccgctt
     1621 ccgacgctcg acaaggtgtc cgcgttcgtc gcgcggcggc tctacagcga ccgctgcaag
     1681 actgtggcgc cgaagaaggt ccccgagacg cagaatcggc tgctcctcac acccggcggc
     1741 gttcccgtgc ggtcgtaccg gcccgccggc atcgcaaaga ccgacgtcct cccggcgctg
     1801 gttttcttcc acggcggcgg ctggaccatc ggcgacctgg atacccacga cgttctctgt
     1861 cgccagcttg cgagcggcgc ccgctgtgag gtgttctccg tcgaatatcg ccttgcaccg
     1921 gaagcgccgt ttcctgctgc ggtcgacgac tgcatcgccg cgaccgaata tgtttccagc
     1981 atcacgaagt ccaatcgcat cgccgtcggc ggtgacagcg ccgggggcaa tcttgcggcc
     2041 gtcgttgcgc tgcatctcaa gcaactgctt tgcttccagc tcctgatcta tcccgcgacc
     2101 ggccagaaca tggaaagtga gtccctgcgg cgcaacggcg agggctatct gctgaccctg
     2161 ccgatgatgg agcgcttccg ctccaactac ctgccgcgcc cggcggacta ccacgactgg
     2221 cgtgcctcgc cggcactcgc aagcagccac gcgggcctgc cgccggcttt cattctcacc
     2281 gccgggttcg atccgctgct ggacgaaggg cggcagtacg cggagctgtt ggcgcgcgcc
     2341 ggtgtcgagg tcgcctatcg cgagtatccc gacatgatcc acggcttcat cctgatgggc
     2401 ggtgcgctcg acactgcgaa cgaggctgtt gctgagtgct gcgttcagct caggaaggca
     2461 ttcgaaaagg tcaccgcatg aaatccgatc tgaagacgcg ccacggcggc aggatcctcg
     2521 ccgacgcgct cgctgcgcag ggcgtaaagc tggcgttcgg cgttcccggc gagagctatc
     2581 tgccggtgct cgatggcctg cacgacctgc aggaccggct gcgcttcgtg atctgccgcc
     2641 aggagggcgg cgcgtcctac atggccaagg cctacggcaa gctcacgggc gagccgggtg
     2701 tgctgttcgt gacgcgcggc cccggcgcga ccaacggcgc gatcggcgtg cacaccggcc
     2761 tgcaggactc gacgccgatg gtggtgttca tcggacaggt gccgaacgaa tttgccgagc
     2821 gcgaagcgtt ccaggagatc gactaccgcc gcatgtatgg ccagatggcg aagtgggtgg
     2881 ggcagatcga ccgcgtcgag cgcatccccg agtacgtgag ccgcgccttc cataccgcgg
     2941 cggcgggccg gcccgggccg gtgatcctcg cgcttcccga ggatgcgttg ttctcggaag
     3001 ccgccgtcgc cgacgtcccg aagcatcatg tggtccgccc ggcgccgtcg ccattgcagg
     3061 tgaatgagct aaaaaaatta ttggagaggg caaagactcc cttcgtgctt ctcggcggcg
     3121 gcggctggga ccgcagcacc acacaaaagc ttcaaaaatg gattgaagcg aacgggctgc
     3181 aggccggcac ttctttccgt tgccaggacc tgttcgacaa ccgcagcccg agctacgccg
     3241 gcgacgtcgg catcggcatc aatccgaagc tcgcgcaacg ggtgaaggaa gccgacgtgc
     3301 tgctagtgat cggcgcgcgc ctcggcgaaa tgacgacgag cgcctacacc ctggtcgaag
     3361 caccggtgcc gcagcaaacg ctggtccacg tgcatgcggg cgccgaagag ctcggccgcg
     3421 tgtaccggcc cgcgctcgcc atcaattcag ggatggctca gttcgtcgac gcggtgcagg
     3481 gcctttcttt caagaatcct gcctggaagg cgcaggccga ggaagcgaga aagcaattcc
     3541 tggaatggac cgagccgcgg ccgatgccgg gcaaggtgca atacggcgag atcatccgct
     3601 ggctctcgga ccggttgccg gaggacacca tcatcgccgg cggcgcgggc aacttcgccg
     3661 gctggctgca ccggcatttc cgctacaagg gctttcgcac ccagctcggc tcgaccaacg
     3721 gctcgatggg ctacggctac ccggcggccg tcgccgccaa gctcgcccag ccgcagcgca
     3781 ccgtgttcgc gctgtgcggc gacggcgact tcctcatgac cgggcaggag atcgcgaccg
     3841 ctgtgcagta cggcgccaac ttcgtcgcgg tcgtcgtcaa caacgggctc tacggcacga
     3901 tccgcatgca ccaggagcgc gagtacccgg gccgcgtcta tggcaccgag ctgcgcaatc
     3961 ccgacttcgc ggcgtacgcg cgcgctttcg gcggccacgg ggagaccgtg gagcgcacgg
     4021 ccgatttcgc gccggcctac gagcgcgccg ccgcctcggg caagccggcc atcgtcgagc
     4081 tgaagatcga tccggacgcg atcacgccgg cgaccacgct ctccgcgatc cgcgagaaag
     4141 cgcttaagtc aaagcaatga acttcgggtc gtcctgctgg gcgcgctcga acttgaggaa
     4201 ctcgtagtag ccgcagcgcg ggctgtccgg gaagttgcgg cacacccccg gccgcgcctc
     4261 gtacacggtg cagcggcgtg tcttctggtc gaggaaggtg cagaccgtct tgaagatgcg
     4321 gtcctgccgg tggcgtagct cccgcacctt ttctttcgcg tcgtatttgg tgaagcgctc
     4381 ctctgcctcg cggtaaggca ggtcgaagtg cttcgccagg cgctcgatgt cgcgcggtgt
     4441 gacctcgatc tcgggatagc tgcagcagta ggcggggcac ttcgagcacg agtattgcgc
     4501 gcgtactgga atcggaaaag ccttgccgtt cttcatggtt ccttaaggaa gcgcggattc
     4561 tacgcgagcg ccacgggaat tatcaccttt aaccctttcg tgttgcgttt tcgtttcacc
     4621 ctggattgcc accacgaaag ggaagaaatg aaaacgctaa gaatggtagt tgccgcagcg
     4681 acaatgatga ccggcacgca tgccctcgcg cacggccggc ttgtcgattt gtccgtttat
     4741 gaccgtagcg aaggtcgatg gctgcaggtc taccggcacg aagggcgcgc gtacgtcgtc
     4801 ggcaagcccg gccacgagta ccgggtccgg gttgccaacc gcctgggcga agacttgctc
     4861 gcagtcgtgt cggtcgacgg gatcaacgtg atcaccggcc agaccgccga cccctcgcag
     4921 agcggctacg tcgtcgcgcc gcgtggggtg ctcgacatga acggctggcg ccggagccta
     4981 gcgcagaccg ccgcgttcta cttcaccgcg ttgcccgact cctatgccgc gcgcaccggc
     5041 cggccggacg acgtcggtgt catcggggtc gcgcttttcc ggaagaaatc ccaacccatc
     5101 gcgaggtccg cgcccgaagt cgccgcgtca gggccccggg cggcacagga gcgtgacgcc
     5161 tcgcggctcg gcaccggcca cgggcgccac gagcattcgc cggccgtgca cgtcaccttc
     5221 gaacgcgcca ccagctctcc tgccgaggtc atcgtgctgc actacgacag ccatgcgaac
     5281 ctcgtggcgc gcggcgtgat ccgcgagccc gttcaggtgg cgcccacgcc gcggccattc
     5341 ccgggattcg tgccggatcc ccaagggtaa actcatgagg aatggaggcc ccggatgaag
     5401 agctcatgct cgcctacggc cgaggcgacg ccggggcctt cgagacgctc tacaagcgtc
     5461 accggggccc ggtctatcgc ttcgtactgc gcgccatcaa gaacaggtcc accgccgagg
     5521 agctgttcca ggaggtctgg atccgggtga tcgaggcgcg cagccgctat acgccgcagg
     5581 cgcgcttcac cacctggctc tacaccatcg cgcacaatct gctcgtggac cactggcgcc
     5641 gcaagggcct gacactcgtg cagctcgacg aggagcaact cgtcgccgcg ccggacaacc
     5701 ctgcgcgaca ggccgaggcg cgcgaggcgc tcacgcggct attgcaggcg atcgagtcgc
     5761 tgccggcggc gcagcgcgag gccttcctgc tccacgagga agccggcttg agc
//
LOCUS       DATBSZ010000133         5048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2273084, whole genome shotgun sequence.
ACCESSION   DATBSZ010000133 DATBSZ010000000
VERSION     DATBSZ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2273084"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VIG70_08575"
     CDS             complement(<1..346)
                     /locus_tag="VIG70_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250647.1"
                     /translation="MSAAAKLEHYVSQAPFEPHAAETLTPEQERFYRASQWQIMWWKF
                     RRHRIAVIAGAILLLFYASTLVSEFIAPYDLHTRDTNHIYAPPQRLHLFHDGSFVGPF
                     VYGYSMRLNMQTL"
     gene            complement(480..1505)
                     /locus_tag="VIG70_08580"
     CDS             complement(480..1505)
                     /locus_tag="VIG70_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8250648.1"
                     /translation="MLRYTLRRVLIMIPTLVVISFVTFVIIQLPPGDFLSNQIAELQS
                     QGDKAAMEKAEFLRKQYGLDKPFLEQYAIWIGAWPGENGLSGLLQGDLGWSFEHDLPV
                     KDVVGDRLLLSFILNFSVILFTWAVAFPIGFYAATHQYSWGDHGLTFLGYVGLATPNF
                     LLALVLMYFANVQFGLSVGGIMDPEYLGQPWSVGKAISVMEHLWIPVIVIGTSGTAGM
                     IRRLRANLLDELQKPYVVTARSKGMAEFPMLVKYPLRMSLNPFIADIGNLLPSVISGS
                     AIVAVVLSLQTSGPMLLEALRSQDQYLAGSFLMFLSLLTVIGMFISDLLLAALDPRIR
                     LTGGVAK"
     gene            complement(1609..3474)
                     /locus_tag="VIG70_08585"
     CDS             complement(1609..3474)
                     /locus_tag="VIG70_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8250649.1"
                     /translation="MRKFLLGIFAALPAMVIAQSLIEPPMLRLDVSTGKLPPVEQRLP
                     QQPLVVRVDQIGKHGGTLHSLVGRSRDTRLLVVYGYARLVGYDREFNLVPDLLESLEV
                     QEGRVFTLKLRKGHRWSDGQPFTAEDFRYYWEDVATNKELLPSGPPIDLLVDGQQPKF
                     EVLSDTVVRYSWHKPNPHFLPRQAGASPLFIYRPAHYLKPFHKKYSEKVRKAEAEGTA
                     KRKWSAVHNRADNLYESDNPDLPTLQPWMNTTRPPADRFVAVRNPYFHRVDEHGRQLP
                     YIDRFILAVVDPKLIPAKTGAGEADLQARDVHFNNYTFLKQGERQNGYRTLLWRPGKG
                     SHFALYPNLNANDPVWRQLLRDVRFRRALSLAIDRSLVNQVLYFGLAIESNNTVLPQS
                     PLFREAYRDRWARYDRKQASQLLDEIGLKRGRDGVRRLPDGRPLEIIVETAGESTEHT
                     DVLELIRETWREVGIKLFSKPSQREAWRNRIFSGETVMSVWSGIENGLATPDNAPDEL
                     APTSQSQHQWPKFGQFLETAGKSGEPVDIPEVKELQRLYKMWLESTTRQDRERAWHAM
                     LELHAEQQFTIGVVSGVPQPVVARDTLMNVPQQGFYNWDPGAFFGIYRPDTFWFK"
     gene            complement(3504..>5048)
                     /locus_tag="VIG70_08590"
     CDS             complement(3504..>5048)
                     /locus_tag="VIG70_08590"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8250650.1"
                     /translation="RISIIFQEPMTALSPLHTIGNQIGEALALHRELGKAERREAVID
                     MLRRVGFPDPVKAEHTYPFELSGGLRQRAMIAMALVCRPALLIADEPTTALDVTIQAQ
                     ILQLIKALQAEFHMAVLLITHDLGVVANMADEVVVMHRGRVMESGSLTDIFREARHPY
                     LKALMRAVPRFNMKPGERLTPIREVQVTQDHLITTGVRPRPNLDGALLEVRNLTKRYA
                     TRKRSFLGAKSSGDFLAVDDVSFQVGPGECLGLVGESGCGKTTTARMILRSVTPDSGE
                     VLFKNEKVLELKGAPLFAYRRKVQFVFQDPFSSLNPRMTAYDIVSEPLVIHGIGDEDE
                     RFARVKEMMGLVGLDVRHLRRYPHSFSGGQRQRIGIARALALGPELLICDEPVSALDV
                     SVQAQVLNLLADLRSALGLAYIFISHNLAVVNYLADRIAVMCAGRIVELAPAAELFRN
                     PVHPYTRALLAAVPEPALDRKLDFDALMEGKASDPAAWPAEFRIAGSQRPGMQDIGRG
                     HLVRAA"
BASE COUNT          861 a         1603 c         1733 g          851 t
ORIGIN      
        1 tcagtgtctg catgttgagg cgcatgctgt agccgtagac gaacggcccg acgaagctgc
       61 cgtcgtggaa gagatgcagc cgctgcggcg gcgcgtagat atggttggtg tcgcgcgtgt
      121 gcaggtcgta aggcgcgatg aactcgctga ccagcgtcga ggcgtagaag agaagcagga
      181 tcgcgccagc gattacggcg atgcggtggc ggcggaactt ccaccacatg atctgccact
      241 gcgaagcgcg gtagaagcgc tcctgctcgg gcgtgagcgt ttcggccgcg tggggctcga
      301 acggcgcctg cgagacgtag tgctcgagct tggcggcggc gctcatggtt tgaccccctg
      361 ataagggggt cgtgagccga aggcgaacgg ggggtttggt ttcgcgagaa agcgaacccc
      421 ccgctcgcga aatacccgct cgcttccccc cttatcaggg gggcaaaacg gcagcgagct
      481 catttcgcca ctccgccagt gaggcgaatc cgaggatcca gggcagcgag cagcaaatcc
      541 gaaatgaaca tcccaattac ggtcaaaagc gagagaaaca tcaagaacga gccggcgagg
      601 tactggtcct ggctgcgcag cgcttccagc agcatcggcc ctgaagtctg cagcgacagc
      661 accacggcga cgatcgccga gccggaaatg acgctcggca gcaggttgcc gatatcggcg
      721 atgaacgggt tgagcgacat gcgcagcggg tatttcacca gcatcgggaa ctcggccatg
      781 cccttcgagc gcgcggtcac cacgtagggc ttctgcaatt catccagcag gttggcgcgc
      841 agccggcgga tcatgcccgc ggtgcccgag gtgccgatca cgatcaccgg gatccacagg
      901 tgctccatga ccgagatcgc cttgcccacg ctccagggct ggccgagata ctccggatcc
      961 atgatgccgc cgaccgagag gccgaactgc acgttggcga agtacatgag caccagcgcg
     1021 agcaggaagt tcggcgtggc gagtccgacg tagccgagga aggtgaggcc gtggtcgccc
     1081 cagctgtact ggtgggtcgc ggcgtagaag ccgatcggga acgcgaccgc ccaggtaaag
     1141 aggatcaccg agaagttgag gatgaacgac aggagcaggc ggtcgccgac cacgtccttc
     1201 accggcaggt cgtgctcgaa cgaccagccg aggtcgccct gcagcaggcc cgagagcccg
     1261 ttctcgcccg gccaggcgcc gatccagatc gcgtactgct cgaggaacgg cttgtcgagg
     1321 ccgtactgct tgcgcaggaa ttccgccttc tccatcgccg ccttgtcgcc ctggctctgc
     1381 agctcggcaa tctggttgga gaggaagtcg cccggcggca gctggatgat gacgaaggtg
     1441 acgaagctga tcacgacgag cgtcggaatc atgatcagca cgcggcgcag cgtgtatctg
     1501 agcatcagta ccgtcgttcc cgcgaacgcg ggaacccatg ttcttgagaa aaattgggcc
     1561 cccgctttcg ccggggcgac gtcctgcctt cgttaaccga aaccaggctc atttgaacca
     1621 gaacgtgtca ggacgataga tgccgaaaaa ggcgccggga tcccagttgt agaacccctg
     1681 ctgcggcacg ttcatcagcg tgtcgcgcgc gaccacgggc tgcggcacgc cggagacgac
     1741 gccgatcgtg aactgctgct cggcgtgcag ctcgagcatg gcgtgccacg cgcgctcgcg
     1801 gtcctgccgc gtcgtgctct ccagccacat tttgtacagg cgctgcagct ccttcacctc
     1861 ggggatgtcg accggctcgc ccgacttgcc ggcggtctcg aggaactggc cgaacttcgg
     1921 ccactggtgc tgcgactggc tggtgggcgc gagctcgtcg ggggcgttgt cgggcgtggc
     1981 gagcccgttc tcgatcccgc tccacaccga catcacggtc tcgccggaaa agatgcggtt
     2041 gcgccaggcc tcgcgctgcg agggcttgct gaagagcttg atcccgacct cgcgccaggt
     2101 ctcgcggatg agctcgagca cgtcggtgtg ctcggtcgac tcgcccgcgg tctcgacgat
     2161 gatctcgagc ggccgcccgt cgggcagccg gcgcacgccg tcgcgtcccc gcttgaggcc
     2221 gatttcatcc agtagctggg acgcttgctt gcggtcgtag cgtgcccagc ggtcgcggta
     2281 cgcctcgcgg aagagcgggc tctgcggcag caccgtattg ttcgactcga tcgcgaggcc
     2341 gaagtagagc acctggttga cgagcgagcg gtcgatggcg agcgagagcg cgcggcggaa
     2401 gcgtacgtcg cgcagcaact ggcgccacac cgggtcgttc gcgttcaggt tcggatagag
     2461 cgcgaaatgc gagcccttgc ccggccgcca caggagcgtg cggtagccgt tctgccgctc
     2521 gccctgcttg aggaaggtgt agttgttgaa atgcacgtcg cgcgcctgca ggtcggcttc
     2581 gccggcgccg gtcttggcgg ggatcagctt cgggtcgacc accgcgagga tgaagcggtc
     2641 gatatagggg agctggcggc cgtgctcgtc gacgcggtgg aaatacgggt tgcgcaccgc
     2701 cacgaagcgg tccgccggcg ggcgcgtggt gttcatccag ggctgcagcg tcggcaggtc
     2761 ggggttgtcg gactcgtaga gattgtcggc gcggttgtgc accgccgacc atttgcgttt
     2821 cgccgtgccc tcggcttcgg ccttgcgcac cttctccgaa tacttcttgt ggaacggctt
     2881 gagatagtgc gccgggcgat agatgaacag cggcgacgcg cccgcctggc gcggcaggaa
     2941 atgcggattc ggcttgtgcc aggagtaacg caccaccgtg tcggacagca cctcgaactt
     3001 cggctgctgc ccgtcgacca ggagatcgat cggcggtccg gacggcagca gctccttgtt
     3061 cgtcgccacg tcctcccagt agtagcggaa atcctcggcg gtaaacggct gcccgtcgga
     3121 ccagcggtgc cctttgcgca gcttgagcgt gaacacccgt ccttcctgca cctcgaggct
     3181 ctcgagcaga tcgggtacga ggttgaactc ccggtcgtag ccgacgaggc gggcatagcc
     3241 gtacaccacc agcaggcgcg tgtcgcggct gcggccaacg aggctgtgca gcgtgccgcc
     3301 gtgctttcct atctggtcga cgcgcaccac gagcggctgc tgcggcaggc gctgctcgac
     3361 cggcggcagc tttccggtgg agacgtcgag ccgcagcatc gggggttcga tcaggctttg
     3421 agcgatgacc atcgcaggca gcgccgcaaa gattcccagc aggaatttcc tcatgctgcg
     3481 gctcatacga tttggcggcg gcttcacgcc gccctcacca aatgcccgcg gccgatgtcc
     3541 tgcatgccgg gtctttggct gccggcgatg cggaattcgg caggccatgc ggcgggatcg
     3601 gacgccttgc cttccatcag cgcgtcgaag tcgagcttgc ggtcgagggc gggctccgga
     3661 accgcggcga gcagcgcccg ggtataggga tgcaccgggt tgcggaacag ctccgccgcg
     3721 ggggcgagct cgacgatgcg cccggcgcac atcaccgcta tgcggtcggc gaggtagttc
     3781 acgactgcga ggttgtgcga gatgaaaata taggcgaggc ccagggcgga gcgcaggtcg
     3841 gcgagcaggt tgagcacctg cgcctgcacc gagacgtcga gcgccgacac cggctcgtcg
     3901 cagatgagga gctcggggcc gagcgcgagc gcgcgcgcga tgccgatcct ctggcgctgc
     3961 ccgcccgaga agctgtgcgg atagcgccgc aggtggcgca cgtccaggcc gacgagcccc
     4021 atcatttcct tcacccgcgc gaagcgctcg tcctcgtcgc cgatgccgtg gatcaccagc
     4081 ggctcgctca cgatgtcgta cgcggtcatg cgcggattga gcgagctgaa cgggtcctgg
     4141 aacacgaact gcaccttgcg ccggtaggcg aacagtggcg cccccttcag ctccagcact
     4201 ttttcgttct tgaagagaac ctcgcccgag tccggcgtga ccgaccgcag gatcatgcgc
     4261 gccgtggtcg tcttgccgca gcccgactcg ccgaccaggc ccaggcattc gcccggcccg
     4321 acctggaagc tcacgtcatc gaccgcgagg aagtcgccgc tcgacttggc tccgagaaac
     4381 gagcgcttgc gcgtcgcgta gcgcttggtg aggttcctga cctcgagcaa ggcgccgtcc
     4441 aggttggggc gcggacgcac cccagtggtg atgagatggt cctgggtgac ctgcacctcg
     4501 cggatcggcg tcaggcgctc gcccggcttc atgttgaagc gcggcacggc gcgcatcagc
     4561 gccttgaggt acggatggcg cgcctcgcgg aagatgtcgg tgaggctgcc cgattccatc
     4621 acgcggccgc ggtgcatcac caccacctcg tcggccatgt tcgccaccac gccgaggtcg
     4681 tgcgtgatga gcagcaccgc catgtggaac tccgcctgca gcgccttgat caattgaagt
     4741 atctgcgcct ggatggtgac gtcgagcgcc gtggtcggct cgtccgcgat caggagcgcc
     4801 ggacggcaca ccagcgccat cgcgatcatc gcgcgctggc gcaggccgcc tgagagctcg
     4861 aacggatagg tgtgctcggc cttgaccgga tccggaaagc ccacccggcg cagcatgtcg
     4921 atcaccgctt cgcgccgctc cgccttgccg agctcccggt gcagcgcgag cgcctcgccg
     4981 atctggttgc cgatggtgtg cagcggcgag agcgcggtca tcggctcctg gaagatgatc
     5041 gagatgcg
//
LOCUS       DATBSZ010000134         7062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_155491, whole genome shotgun sequence.
ACCESSION   DATBSZ010000134 DATBSZ010000000
VERSION     DATBSZ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155491"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VIG70_08595"
     CDS             complement(<1..683)
                     /locus_tag="VIG70_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY8250651.1"
                     /translation="MQPTDIRNPDYFHKVVDCQWACPAHTPVPEYIRLIAAGRYSDAY
                     MVNWESNVFPGILGRTCDRPCEPACRRGRVEEENAAKPEPVAICRLKRVAADYKDNEE
                     ISKKISTQKRTKSNGKKIACVGAGPASLTVARDLAPLGYEVTVFDQDPRAGGMIWSQI
                     PRFRLPLEVIDEEVNYILNLGVSFKQRKIDSMKQLMGEGYDAIFVGSGAPRGRDLDIP
                     GRKEGAKNI"
     gene            complement(767..2068)
                     /locus_tag="VIG70_08600"
     CDS             complement(767..2068)
                     /locus_tag="VIG70_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8250652.1"
                     /translation="MHPAAQGLAATNYPIRSAAFLCCYAVVGLVLWERNAGPVAWTLL
                     ALQFIVYPHTVYWRSRLSARPTRAELDNLFLDAALLGAWCAELGFPTWITFAFVTATS
                     LNAMVNRGAQGFAWSLACSVAGAVLWVMVRGLNYMPATSDVVTALCVLGTLGYVGAVG
                     HVVHAQNRRLTSARDELRSSEERYRLIAENAGDLIGMVDHHGRWIYTSPSYARILDPE
                     DLAPGADAFRKVHPDDAEHARAAAMRTSTIVKPRAFGMRLVDKDGRVRQYKTWVHGIA
                     GDGAAPRSLLVSQDVTDLRESEERVLLAAHAFEGLTESIVITAADGTIVTVNRAFCEL
                     TGFTRDDVLGQPETMIRNALQPPEYYQEVFAAIARQGYWSGTTWARRKNGAVYREWRS
                     VRVVRDPAGNVTHYVMVLSEVGASGSRASIDITLPNPNLRG"
     gene            2136..3056
                     /gene="tal"
                     /locus_tag="VIG70_08605"
     CDS             2136..3056
                     /gene="tal"
                     /locus_tag="VIG70_08605"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY8250653.1"
                     /translation="MTQLESLRQHSLVVSDTGDIEAVARWKPQDATTNPSLLLSSAED
                     PRFRHLMDRDVDQVFVNFGCEILRHIPGRVSTEVDARLSFDTARSVEKARRFISLYEK
                     AGVKRERVLIKLAATWEGIKAAEKLEREGIHCNMTLLFSFAQAAACADAGVTLISPFV
                     GRIYDWYRQQRKVDDIPVEDDPGVASVVRIYNYYKKHGYKTQVMGASFRKTGQILALA
                     GCDLLTIAPDLLDKLSKAEGGIARKLSPEAARRSDIDKVALDEKSFRWQHNDDAMATD
                     KLAEGIRRFDADARKLEKLIISLAAAVPAK"
     gene            complement(3122..3304)
                     /locus_tag="VIG70_08610"
     CDS             complement(3122..3304)
                     /locus_tag="VIG70_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250654.1"
                     /translation="MWKKWWLLFAVIWVVVATIQVAVILGTSEESEKALQPAVLGVAV
                     PAVLYLLGWLWARLRR"
     gene            complement(3304..3933)
                     /locus_tag="VIG70_08615"
     CDS             complement(3304..3933)
                     /locus_tag="VIG70_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250655.1"
                     /translation="MGGSKAFVAAAVLVIAGSTGYLLYADKQKKEQQRALAALLGDTT
                     AQLRKALSAPPPADLVSRLDGNLKAAKAPRDRPLADAAGVYIYGAREIVRRRGEAERL
                     AREAAMSRRALAMHMAAASGRDTYWFRVASDLKKRVERDHHELEVSLKALSHLLHTLP
                     ETQKPLESHVDASLLLEDAVRRSAEERAEATAQRAAADLEKVRGLGPSK"
     gene            complement(3996..5012)
                     /locus_tag="VIG70_08620"
     CDS             complement(3996..5012)
                     /locus_tag="VIG70_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY8250656.1"
                     /translation="MLRLKEGWSLLKEAVQAWSNDYAPSMGAALSYYTLFSIAPLLLI
                     VIAVAGWFFGDEAVRGELTAQLQWLMGEEGAKAIEGMIAAASEPKEGMIATLVGLFVL
                     VIGATTVFGELQNALDRIWRAPVQEKTGSVWRLLRARLLSIGMILCLAFLLMVSLVFD
                     AVLQSFGKMWGTGGWEVFAQVLNTLVAFGLTTVVFAMIYKLMPRAKIEWHDVWVGAAV
                     TAILFAAGKFLISLYIGRSAVASSFGAAGSLAVMMIWVYYSAQIFLLGAEFTWVYAHT
                     HGSRRGQERPGDIEEKPVFEPKAPAPVPGHVPVHKKKPLVTFGVAAAIGAIAGGIFSR
                     FKPH"
     gene            5092..5382
                     /locus_tag="VIG70_08625"
     CDS             5092..5382
                     /locus_tag="VIG70_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250657.1"
                     /translation="MARLRVVGFATWCRIIATAALLLVVPAHAQDKPNRIERTAQKAA
                     QGTENTAKRAAKFAEETTHRSAKFVEKTATRAGKFVDRTATKTGNAVKRAVE"
     gene            5418..5666
                     /locus_tag="VIG70_08630"
     CDS             5418..5666
                     /locus_tag="VIG70_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250658.1"
                     /translation="MEQKPRWTEMCARILQQCEVLRGGRQNLALFLEVDPDELGDWVE
                     ARTGPPRSVFEKAMEVILAEHDRRAALAPPPKRRKTDH"
     gene            complement(5711..6007)
                     /locus_tag="VIG70_08635"
     CDS             complement(5711..6007)
                     /locus_tag="VIG70_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250659.1"
                     /translation="MAENDDPKADETLRWPINLDRAAIERRLAEVRTAAQAQGLDLVV
                     KLFSDVSRMSGADLGANVVAALTWLQDKPQHRPITMQLEMVAMNLKNLKKPKKK"
     gene            complement(6008..>7062)
                     /locus_tag="VIG70_08640"
     CDS             complement(6008..>7062)
                     /locus_tag="VIG70_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8250660.1"
                     /translation="IKVQSTWPASITLQDHLRILADRVDKLTQGQVKIEALAAGQIVP
                     PFEVLDATNKKVLDGFHAISYYWVGKSPAAALFAGPPGGPFGMDHMDFLGWLYVGGGL
                     EMWREFYQNDLKLNVTVWPAHPSSPQALGWFKKPLKSVADFKGMKCRQTGLNAEVYAK
                     LGQSVVNMAGGEIVPAAQRGVIDCAEWVGGVEDLRLGLPGVFKYHYTPGMHENNSIGE
                     FGLNLDVWKGFTPQQQQAVNSAINDTFITWITRWQKQNADAIEEMIKKHGVQIRRTPP
                     DILIATLKAWDDVAREHSAKSATFKKVYESQREYAAKVVPAKRYMFPPYSFAANYYWP
                     QEGAGTAAAKKEAKKP"
BASE COUNT         1172 a         2339 c         2367 g         1184 t
ORIGIN      
        1 tggatgttct tggcgccttc tttgcgcccc gggatgtcga ggtcgcggcc acgcggggcg
       61 ccgctgccga cgaagatcgc gtcgtagccc tcgcccatca gctgcttcat cgaatcgatt
      121 tttctctgct tgaagctgac gccgaggttg aggatgtagt tcacctcttc gtcgatcact
      181 tccagcggca ggcggaagcg cgggatctgc gaccagatca tgccgccggc gcgcgggtcc
      241 tggtcgaaca cggtgacttc gtagccgagc ggcgcgaggt cgcgcgccac ggtgagggac
      301 gccgggccgg cgccgacgca cgcgattttc ttgccgttgg attttgttct tttctgagtg
      361 gagatctttt tggagatctc ctcgttgtcc ttgtagtccg cggcgacgcg cttcaggcgg
      421 cagatcgcga ccggctcggg cttggcggcg ttctcctcct cgacgcggcc gcggcggcac
      481 gcgggctcgc acgggcggtc gcaggtgcgt ccgaggatcc cgggaaatac gttggactcc
      541 cagttcacca tgtaggcgtc ggagtaacgc ccggcggcga tcagccggat gtattcgggg
      601 acgggcgtgt gggccgggca ggcccactgg caatcgacaa ccttatgaaa gtagtccgga
      661 ttcctgatgt cggtcggctg caactgtcct gctcctggta actgggtggt tagtaattct
      721 tgttaccgcg agtttacacc aggcacaggc aggaaagcct tacttttcaa cctctaaggt
      781 tcggattggg aagggtaatg tcgatcgacg cgcggctccc gctcgcgccg acctcggaga
      841 gcaccatgac gtaatgagtg acgttgccgg cgggatcgcg caccacgcgc acgctgcgcc
      901 attcgcggta caccgcgccg ttcttgcggc gcgcccacgt ggtcccggac cagtagccct
      961 ggcgcgcgat ggcggcgaac acttcctggt agtactcggg cggctgcagg gcgttgcgga
     1021 tcatcgtctc gggctggccg aggacgtcgt cgcgcgtgaa gccggtgagc tcgcagaacg
     1081 cgcggttcac ggtcactatc gtgccgtcgg ccgcggtgat cacgatcgat tcggtcaggc
     1141 cctcgaaggc gtgcgcggcg agcagcacgc gctcctcgct ctcgcgcagg tcggtgacgt
     1201 cctgcgacac gagcaacgag cgcggcgcag cgccgtcgcc ggcgatgccg tgcacccagg
     1261 tcttgtactg gcgcacgcgc ccgtccttgt cgaccaggcg catgccgaac gcgcgcggct
     1321 tgacgatggt cgaggtgcgc atcgccgcgg cgcgcgcgtg ctcggcgtcg tcggggtgca
     1381 ccttgcggaa ggcgtcggcg ccgggcgcga ggtcctcggg gtccaggatg cgcgcgtagg
     1441 acgggctggt gtagatccag cggccatggt ggtcgaccat gccgatcagg tcgccggcgt
     1501 tctcggcgat cagccggtag cgctcctcgc tgctccggag ctcgtcgcgc gccgaggtga
     1561 ggcgccggtt ctgcgcgtgg accacgtggc cgacggcgcc cacgtagccg agcgtgccca
     1621 ggacgcaaag cgcggtcacg acgtcgctgg tggcgggcat gtagttcagg ccgcgcacca
     1681 tgacccacag caccgcgccg gcgacgctgc aggcgagcga ccaggcgaag ccttgcgcgc
     1741 cgcggttcac catcgcgttc agcgaggtgg cggtgacgaa ggcgaaggtg atccaggtcg
     1801 ggaaaccgag ctcggcgcac caggcgccga gcagcgcggc gtccaggaac aggttgtcga
     1861 gctcggcgcg ggtcggccgc gcggaaagcc gcgagcgcca gtagaccgtg tgggggtaga
     1921 cgatgaactg cagcgcgagg agcgtccagg cgaccggccc ggcgtttcgt tcccacagca
     1981 ccagtccgac cacggcgtag cagcaaagga acgcggccga gcggatcgga taatttgttg
     2041 ctgcgaggcc ttgcgcagca ggatgcatga ttgcacacta agtcacacgc acttccccac
     2101 cttaagaatt tgtcaggccc ctccaaagaa ttgtcatgac ccagctcgaa tccctgaggc
     2161 agcactccct ggtggtctcc gacaccggcg acatcgaagc ggtggcgcgc tggaagccgc
     2221 aggacgccac gaccaacccc tcgctcctgc tttcctcggc cgaggatccc cgcttccggc
     2281 acctgatgga ccgcgacgtg gaccaggtct tcgtgaattt cggctgcgag atcctcaggc
     2341 acatccccgg gcgggtctcc accgaggtgg acgcgcgcct gtccttcgac accgcgcgct
     2401 cggtcgagaa ggcgcggcgg tttatctcgc tgtacgagaa agccggcgtc aagcgcgagc
     2461 gcgtcctcat caagctcgca gccacctggg agggcatcaa ggcggcggag aagctcgagc
     2521 gcgaaggcat ccactgcaac atgacgctcc tcttctcctt cgcgcaggca gccgcctgcg
     2581 ccgacgccgg cgtcaccctg atctcgccct tcgtcggccg catctacgac tggtatcggc
     2641 agcagcggaa agtggacgac atcccggtcg aagacgatcc cggcgtcgcc tcggtggtgc
     2701 gcatctacaa ctactacaag aagcacggct acaagaccca ggtgatgggc gcgagcttcc
     2761 gcaagaccgg ccagatcctg gcgctggcgg gctgcgacct gctcaccatc gccccggacc
     2821 tgctggacaa gctctcgaag gccgagggcg ggattgcgcg caagctctcg ccggaggcgg
     2881 cgcggcgctc cgacatcgac aaggtcgcgc tcgacgaaaa atccttccgc tggcagcaca
     2941 acgacgacgc gatggcgacc gacaagctcg ccgaaggcat ccggcgcttc gacgccgacg
     3001 ctcgcaagct cgagaagctg atcatctccc tcgccgcggc ggtccccgcc aaataattcg
     3061 gggtcagggt gcgaactcgt tttcgcgtac gcaaaaaccg agttcgcacc ctgaccccga
     3121 attaccgcct aagccgcgcc cagagccagc cgagtaagta gaggacggcg ggaacggcca
     3181 cgcccagcac cgcgggctgg agggctttct cggattcttc ggaggttccc aggatcactg
     3241 cgacctggat cgtggcgacg acgacccaga tcacggcgaa cagcagccac cactttttcc
     3301 acactacttc gacggtccca ggccgcggac tttctcgaga tcggccgcgg cgcgctgcgc
     3361 ggtggcttcg gcgcgctctt ccgcgctgcg ccgcaccgcg tcctcgagga ggagcgaggc
     3421 gtcgacgtgg gactcgagcg gcttctgcgt ctcgggcagc gtatgcagga gatgcgacag
     3481 cgccttcagc gagacctcga gctcgtggtg atcgcgctcg acgcgcttct tgaggtcgga
     3541 ggcgacgcgg aaccagtagg tgtcgcgccc gctcgcggcg gccatgtgca tggcgagggc
     3601 gcggcggctc atcgcggcct cgcgcgcgag gcgctcggcc tcgccgcgcc ggcgcacgat
     3661 ctcgcgcgcg ccgtagatgt agacgccggc ggcgtcggcg agcggccggt cgcgcggggc
     3721 cttggcggcc ttcaggttgc cgtccaggcg cgaaaccagg tcggcaggcg gcggcgcgct
     3781 cagcgccttg cgcagttgcg cggtggtatc gccgaggagc gcggccagtg cgcgctgctg
     3841 ctccttcttc tgcttgtctg catacaggag ataccccgtg ctcccggcga tgacgagcac
     3901 ggcggcggcg acgaaggcct tcgaacctcc catgggcggg gaggataccg aattcgggga
     3961 cggagcccga attaattaag gctccgtccc cgaattcagt gcggcttgaa gcgtgaaaaa
     4021 attcccccgg cgatcgcgcc gatcgccgcg gcgacgccga aagtcaccag cggctttttc
     4081 ttatgaaccg gaacatgccc cgggaccggc gccggcgcct ttggctcgaa caccggcttt
     4141 tcttcgatgt cgccgggccg ctcttgtccc ctcctcgagc cgtgcgtgtg cgcgtacacc
     4201 caggtgaatt ccgcgccgag cagaaagatc tgcgccgagt agtacaccca gatcatcatc
     4261 acggcgagcg agcccgcggc gccgaaggac gaggcgaccg cgctgcggcc gatgtagagg
     4321 ctgatgagga acttgccggc ggcaaagagg atcgcggtga ccgcggcgcc gacccagacg
     4381 tcgtgccact cgatcttggc gcgcggcatc agcttgtaaa tcatcgcgaa caccacggtg
     4441 gtgagcccga acgcgaccag ggtgttgagg acttgcgcga acacctccca cccgcccgtg
     4501 ccccacatct tgccgaacga ctgcaatacc gcgtcgaaca cgagcgacac catgagcagg
     4561 aacgcgaggc agaggatcat gccgatcgag agcagccgcg cgcgcagcag ccgccacacg
     4621 ctgccggtct tctcctgcac cggcgcgcgc cagatgcggt cgagggcgtt ctgcagctcg
     4681 ccgaacacgg tggtcgcgcc gatgacgagg acgaagaggc cgaccagggt ggcgatcatg
     4741 ccctccttcg gctcgctcgc cgcggcgatc atgccctcga tcgccttcgc gccctcctcg
     4801 cccatgagcc actggagctg cgcggtgagc tcgccgcgca ccgcctcgtc gccgaagaac
     4861 cagcccgcga ccgcgatcac gatgaggagc agcggggcga tcgagaacag cgtgtagtac
     4921 gagagcgccg ctcccatgct cggcgcgtag tcgttggacc aggcctggac agcctccttg
     4981 agcaacgacc agccttcctt caggcgcagc atgccctcgc tgcgagcagg gaatatgccc
     5041 gccagcatgc gccggttgtt ctcttagaat gctcttcgga ggcgaaaagc catggcgaga
     5101 ctgcgggtag tcggctttgc aacttggtgt cgaataatcg ctaccgccgc cctgctgctg
     5161 gtggtgccgg cccatgcgca ggacaagccc aaccggatcg agcgcacggc gcagaaagcc
     5221 gcgcaaggga cggaaaacac ggcgaagcgg gcggccaagt tcgccgagga gaccacgcac
     5281 cgctccgcga agttcgtcga gaaaaccgct acgcgcgccg gcaagttcgt cgaccggacc
     5341 gcgacgaaga ccgggaacgc ggtcaaacgg gcggtggaat agaaagaacc tgcggcgcgg
     5401 gcgagtgtct cttgcccatg gaacaaaaac cgcgctggac cgaaatgtgc gcccgcatcc
     5461 tgcagcagtg cgaagtgctg cgcggagggc ggcagaacct cgccctgttt ctcgaggtcg
     5521 atcccgacga gctcggcgac tgggtcgagg cccggacggg cccgccgcgc tcggtgttcg
     5581 agaaggcgat ggaagtgatc ctcgccgagc acgaccgccg cgccgcgctc gccccgccgc
     5641 cgaagcggcg caagaccgac cactgaaatt cggggacgga gcccgaacta gttagggctc
     5701 cgtccccgaa ttacttcttc ttcggcttct tcaggttctt caggttcatc gcgaccatct
     5761 cgagctgcat cgtgatgggg cgatgctggg gcttgtcctg cagccaggtg agggcggcga
     5821 ccacgttggc gcccaggtct gcgccgctca tgcgcgagac gtcggagaac agcttgacga
     5881 ccaggtcgag cccctgcgcc tgcgccgcgg tgcgcacctc cgccagccgt cgctcgatcg
     5941 cggcgcgatc gaggttgatc ggccagcgca gggtctcgtc agccttggga tcgtcgttct
     6001 cggccaatca gggcttcttc gcctctttct tggcggcggc ggtgccggcg ccttcctgcg
     6061 gccagtagta gttcgcggcg aacgagtacg gcgggaacat gtagcgcttc gccggcacca
     6121 cctttgcggc gtactcgcgc tgcgactcgt agaccttctt gaaggtcgcg ctcttcgccg
     6181 agtgctcgcg cgccacgtcg tcccacgcct tgagcgtggc gatcaggatg tcgggcgggg
     6241 tgcggcggat ctgcacgccg tgcttcttga tcatctcctc gatggcgtcg gcgttctgct
     6301 tctgccagcg cgtgatccag gtgatgaatg tatcgttgat cgccgaattc accgcttgct
     6361 gctgctgcgg cgtgaagccc ttccagacgt cgaggttcag gccgaattcg ccgatcgagt
     6421 tgttctcgtg catccccggc gtgtagtggt acttgaacac gcccggcagg cccaggcgca
     6481 ggtcctcgac cccgccgacc cactcggcgc agtcgatcac gccgcgctgc gcggccggca
     6541 cgatctcgcc gccggccatg ttgaccacgc tctggccgag cttggcgtag acctccgcgt
     6601 tcaaacccgt ttgccggcac ttcatgccct tgaagtcggc caccgacttg agcggcttct
     6661 tgaaccagcc gagcgcctgc gggctcgacg ggtgcgccgg ccacaccgtc acgttcaatt
     6721 tcaggtcgtt ctggtagaac tcgcgccaca tctcgagccc gccgccgacg tagagccagc
     6781 cgaggaagtc catgtggtcc atgccgaacg ggcctccggg cggcccggcg aacagcgccg
     6841 ccgccgggct ctttccgacc cagtagtacg aaatggcatg gaatccgtcg agcactttct
     6901 tgttggtagc atcgaggacc tcgaacggcg gcacgatctg gccggcggcg agcgcctcga
     6961 tcttcacctg gccctgcgtc agcttgtcga cgcggtcggc gaggatcctg aggtggtcct
     7021 gcagcgtgat gctcgcgggc caggtggact ggaccttgat gg
//
LOCUS       DATBSZ010000135        11261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_702473, whole genome shotgun sequence.
ACCESSION   DATBSZ010000135 DATBSZ010000000
VERSION     DATBSZ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11261
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702473"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1281)
                     /locus_tag="VIG70_08645"
     CDS             complement(73..1281)
                     /locus_tag="VIG70_08645"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEY8250661.1"
                     /translation="MLDAYLYDGLRTPIGRHAGKLAPVRPDDLAAEVLKEVVARNKIE
                     PAEISDVVMGCVTQAGEDSRNVARFAALLAGLPPTTPGVTVNRLCASGLQAVVDAARA
                     ITCGEGDLYVAGGVESMSRAPYVMGKSDSPFSREVKMFDTTIGARFPNPKVVRQFGNH
                     TMPETGDNVAKDFGITREQADEFALASQQKYAKAEAEGFYKGEIHGVALPGRKKDAPP
                     TVVDADEHPRRDSTMDSLKKLKPLFEGGVVTAGNASGVNDGAAALIIGSKSWGEKKGK
                     KPIARILSAASAGVEPRIMGVGPAYAIPLALERAKLKLSDVDIIEINEAFASQVLGCL
                     KLMKVDFKDPRVNPNGGAIAIGHPLGCSGARLALTVARELQQSGGRYAAVSLCIGVGQ
                     GLAVILERMQ"
     gene            complement(1294..2790)
                     /locus_tag="VIG70_08650"
     CDS             complement(1294..2790)
                     /locus_tag="VIG70_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY8250662.1"
                     /translation="MLDPNRSDLVAGVAGAGTMGRGIAQVLAQCGARTLLFDAQPGAA
                     RRAKDSIAQALARLVEKGRLRAAEADTSVGRIEIIDSVEGLAPCHVVVEAIVEDLGAK
                     QKLFSSLEQAVKGDCILASNTSSLSVTAMAAACKRPGRVAGYHFFNPVPVMKVVEVVD
                     GVLTEPWASDALTALARRFGHTPVRCKDTPGFIVNHAGRAFVPEALRVLSEGIADFAT
                     IDRILVDAAGFRLGPFGLMDLVGLDVAHAVMKSMYQQYFEEPKYRPSFLAEPRVAAGL
                     LGRKTGRGWYEYDKNSAALRTPEAPAPGARPNAVWAVPELKELMVELGARPELKPTPH
                     AVCFVAPFGADATTSALQLGLDPVRTVAVDPLFGFSKRRTLMKTPVTSETTVQSAHAL
                     LAADGVPVSVINDSPGFVAQRVVSHIVNVGCDIVQMRIATPEDLDRAVTLGLGYPRGP
                     LAMGDAVGPKRILAVLEAMHDFYQEPRYRASPWLRRRAALGVSLLMEE"
     gene            2845..3789
                     /locus_tag="VIG70_08655"
     CDS             2845..3789
                     /locus_tag="VIG70_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250663.1"
                     /translation="MKRIFSLAAGLLLAFAAQAQQFPTKPVTLIVPWPAGGSSDIYFR
                     RLGEVAARHLGQPLVIENRPGGSGMNGPATMTKTAKPDGYTISQLVITAFRMPHMQKV
                     DWDPINDFTYIIGLAGYTFGVVVRADSPFKTFNDLIAYARANPGKLSYATPGTGTSLH
                     LAMEEIGAKANVQFLHVPFKGYGDGAIALMGGHVMVQVDSTGWAKQVDTGAQRLLATL
                     GDKRTRWNAPTVKELGVDTVSNSPYGLVGPKGMPREVVKVLHDAFKKSLDDPEYLKLL
                     AMLDQPAWYMSSEDYVRWAADMLKAERATIERVGLLLK"
     gene            3800..4240
                     /locus_tag="VIG70_08660"
     CDS             3800..4240
                     /locus_tag="VIG70_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8250664.1"
                     /translation="MKYRKILLAYNGTKEGKRALLECADLASFLQAETHLLAVASMPP
                     SLFLTEGFVPEELLEEEKKRTQSVLDEGIRTLRDRGFNATGQLAVGEPVEEICRLAKS
                     LGVDLIVLGHNQNTSFAARWWKGSVGASLLDYSPCSLLIAMSKG"
     gene            4258..6531
                     /locus_tag="VIG70_08665"
     CDS             4258..6531
                     /locus_tag="VIG70_08665"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8250665.1"
                     /translation="MLKGRLEDDRMLTGRGRYVSDWNLPNQAYAHFLRSDRAHAEIVS
                     IQKGDALKSKGVIAVFTGDDLDLKSLPAALPAKGRGGTELINPGRPALAKGRVRFAGD
                     AVALIVAESAVAAQDAAELIAVEYRDLPAVVKTGDALAAGAPLVHDSVPGNMVLDYES
                     GDEAGARAAFESAARIVELTVDISRVVGNPMEPRACLAAFVSDQYHLYTCTQGVAIMR
                     NQLSAVLGVPPEQIRVIAEEVGGGFGVRFNLYPEYCTALFAAKKLGRPVKWTGTRSEV
                     FLADEQARDVRSRGELALDGQGRILGMRFDMVANLGAYLAPTGPFINTIGIVNCLSGV
                     YDVPATYARIRLALTNTAPMAAYRGAGRPIMSYAIERLIDHAARELGLDPTELRRRNF
                     IPKHKFPYKIAAGFEYDCGDFAGVLDKALQASSWDSFDQRKTEAKRRGRLRGRGIATY
                     IEATGAGFAPHDQVELRWDEGGFDSGGRITVYAPTHNHGQGHETTYAQLLSGVLGLPL
                     EAFRLRTAGPEFYIAGNATGGSRSLLAVGSVVMLAGHEMVKKGMALAADELEAAVADL
                     EFVQGNYRIKGTDRKISIQSLARQQPGNLDIQYQNKFGATFPNGCHIAEVEIEPETGD
                     AEIVSYVACDDAGNIINHQIVEGQVQGGLTQGAGQVFGELAVYDRSGQLLTGSFMDYA
                     MPRAGLVNGIKLLDHPVPTKLNPLGAKGVGEAGVTGSLPALMNAVVDALRQAGVREFE
                     MPATPARIWKAIQEAKN"
     gene            6734..7915
                     /locus_tag="VIG70_08670"
     CDS             6734..7915
                     /locus_tag="VIG70_08670"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEY8250666.1"
                     /translation="MRRSSERILTTHVGSLIRPQSLLPLIGAKQSGQPYDRELYSKRL
                     AESVAEVVAQQAAAGIDVPSDGEFGKSISWSQYALERLSGFERRPVAAGASRFARGAD
                     RTRFAEFYVELDAASGPPATAGASAGEAVCVAPIRYTGQAEVQRDIANFKAALARAGV
                     AEGFLPVAAPASVIPDRRNEHYPSDEKMLEAIADAMRTEYRAIVDAGLLLQLDDARAA
                     VTYDRMVPPASFADYRSWLERQVEAINRATEGIPSDRIRYHVCWGSWPGPHTSDVPLK
                     DIVDLVLRVRAGAYLIEAANPRHEHEWRVWEKARLPEGAVLVPGVISHATNVVEHPEL
                     VAERIVRFARLVGRENVIAGTDCGFAQGPFHRRVHPSIMWAKLEALAEGARLASRELW
                     N"
     gene            complement(7912..8244)
                     /locus_tag="VIG70_08675"
     CDS             complement(7912..8244)
                     /locus_tag="VIG70_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250667.1"
                     /translation="MKHADLQKVRGTLAGMGAVLAKSHGGADDDESLLEFRGLCWEAM
                     LLADDGECQEQIDLLVQCAKDLYSERDHRRVGTLRGEIDRALGALRARLDSLERGYGK
                     RWRDLRAA"
     gene            complement(8359..8877)
                     /locus_tag="VIG70_08680"
     CDS             complement(8359..8877)
                     /locus_tag="VIG70_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250668.1"
                     /translation="MKARKVPVFGVALVVAGLALVLYFVALFAWQITASIQIRSWIAL
                     PATLLFSDHSLLQAAKAAPVLEFIPEVPWPWLMNPDAWLPVHSAVTWILTRVHVGLPF
                     ALVGLPVMALGVLAAMRRKAVFHAQRQQYEDRLRRTHDYRREDHPESLDGRREPFIGA
                     GSIAGNAERRVA"
     gene            complement(8970..9971)
                     /locus_tag="VIG70_08685"
     CDS             complement(8970..9971)
                     /locus_tag="VIG70_08685"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY8250669.1"
                     /translation="MSITTLTRKIAGLAHRLWRRAPPAPGRFSLSRKSSAHGFIGAAP
                     APPWREYLLYLPRNFDARERRSLVVWIHGCRQDPEAFAAGTRIARFADERGFVVLLPR
                     QSRLANSERCWNWFDRRTAGGAGEAAIVAAQVAEVVEKFRLHPERVYVAGLSSGGTLA
                     ATLALRAPHLFAAVAVHSAVACGAASDAADAARVMAKGPFGGTDALGAEARARTAGKA
                     RVPLLVIHGSADDTVAPVNAAFLVRQFLIFNGIAPQELPAGAALPPAQIRAFQPRADY
                     LLSEYHAGRRLAAQLLTVPGLGHAWSGGDPAYPFFDSRHLDATKLVCDFFASHSRQP"
     gene            10010..11044
                     /locus_tag="VIG70_08690"
     CDS             10010..11044
                     /locus_tag="VIG70_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY8250670.1"
                     /translation="MRTLLGIATAALLLVPAAAQAAAEFKIATADKRGTYFAIGADLA
                     KFVAPGADISLEVMPTDGSAANIRHLRFDAGVKFAIVQADVYQAFVDRGGANRDAARI
                     IGPLRVILPLYNTEIHYIARADSPLNYLHDLKDAKINGGLVGSGAAFITHTLYRMMFN
                     SAMPEANASYMPNDEALIKLITDKSIDVAVVAAGQPAPIIANMKPEAQKYIKLLKFDP
                     SHPASRSALSIYAPATVHAKSYPNLLASDFTTISVGAFLVTYDYNLQTTVDYLARFAR
                     SLCQNFATLQAKGHPKWREVDLTLPALNKGWTYYPPTSREIRNCLADRSKAGRAKACS
                     AEERILGFCN"
BASE COUNT         1922 a         3808 c         3774 g         1757 t
ORIGIN      
        1 cgtcctgggt gagctgctgc tcgagcagca gcgggtcgtc ccacacgaag cccgctttca
       61 cgttctctct tttcattgca tgcgctccag gatgacggcg aggccctggc cgacgccgat
      121 gcacagcgac accgcggcgt atcggccgcc cgactgctgc agctcgcgcg ccaccgtgag
      181 cgcgagccgc gcgccggagc agccgagcgg gtggccgatc gcgatcgcgc cgccgttcgg
      241 gttgacgcgc gggtccttga agtcgacttt catgagcttg aggcagccga gcacctggct
      301 cgcgaaggcc tcgttaatct cgatgatgtc cacgtcgctc aacttcaact ttgctctttc
      361 aagagccagc ggaatcgcgt acgcgggccc tacgcccatg atgcgcggct cgacgccggc
      421 gcttgccgcg gagaggatcc gggcaatggg tttcttccct ttcttttcgc cccacgactt
      481 cgagccgatg atgagcgcgg cagcgccgtc attcacgccc gaggcgttgc cggcggtgac
      541 cacgccgcct tcgaacagcg gcttcagctt cttcagtgaa tccatggtgc tgtcgcgccg
      601 ggggtgctcg tccgcgtcga ccacggtcgg tggggcatcc ttctttctcc ccggcaatgc
      661 aaccccgtgg atctcgccct tgtagaaccc ctccgcctcg gccttggcgt acttctgctg
      721 cgaggcgagc gcgaattcgt cggcctgctc gcgcgtgatg ccgaagtcct tcgccacgtt
      781 gtcgccggtc tccggcatcg tgtggttgcc gaactgcctc accaccttcg ggttgggaaa
      841 gcgcgcgccg atcgtggtgt cgaacatctt cacttcccgg ctgaagggcg aatcggactt
      901 gcccatcacg taaggtgcgc ggctcatgct ctcgacgccg ccggcgacgt acaaatcgcc
      961 ctcgccgcag gtaatggcgc gcgccgcatc gacgactgcc tgcaggccgc tcgcgcacag
     1021 gcgattcacc gtgaccccgg gcgtggtcgg cggcaggccg gcgagcaggg cggcgaagcg
     1081 ggcgacgttg cggctgtcct cgcccgcctg ggtgacgcag cccatcacga cgtcgctgat
     1141 ctccgccggc tcgatcttgt tgcgcgcgac gacctccttc agcacttcgg cggcgaggtc
     1201 gtccggccgc accggcgcga gcttgccggc gtggcgtccg atgggggtgc gcagtccgtc
     1261 gtacaggtag gcatctagca tgggtcgctc tctctattct tccatcagca gtgaaactcc
     1321 gagcgcagcg cgtcggcgca gccaggggct cgcgcggtag cgcggctcct ggtagaaatc
     1381 gtgcatcgct tcaagcacgg caaggatgcg tttcggcccg acggcgtcgc ccatcgcgag
     1441 cgggccgcgc gggtagccca ggccgagggt gacggcgcgg tccaggtctt cgggcgtcgc
     1501 gatgcgcatc tgcacgatat cgcagcccac gttcacgatg tgcgacacca cgcgctgcgc
     1561 gacgaagccc ggcgagtcgt tgatcaccga aaccggcacc ccgtccgcgg cgagcagggc
     1621 gtgggccgat tgaactgttg tttcgctggt gacgggagtt ttcatcagcg tacggcgttt
     1681 ggagaatccg aagagcggat cgaccgccac cgtgcgcacc ggatcgaggc cgagctggag
     1741 cgcgctggtg gtcgcatcgg cgccgaacgg cgccacgaag cacacggcgt gcggcgtcgg
     1801 cttgagctcc ggcctggcgc cgagctcgac catcaattcc ttcaactccg gcacggccca
     1861 cactgcgttc ggcctcgcac cgggcgccgg cgcttccggc gtccggaggg cagcactgtt
     1921 cttgtcgtat tcgtaccagc cccgtccggt cttgcgtccc agaagccccg cggcgacgcg
     1981 cggctcggcg aggaacgagg gccggtattt cggctcctcg aaatactgct gatacatgga
     2041 cttcatcacc gcgtgcgcga cgtcgaggcc aacgaggtcc atcagcccga agggcccgag
     2101 gcggaagccg gccgcgtcga ccaggatgcg gtctatggtg gcgaagtcgg cgatgccttc
     2161 ggacagcacg cgcagcgctt cggggacgaa cgcgcgcccg gcatggttga cgatgaagcc
     2221 cggcgtgtct ttgcagcgca ccggcgtgtg gccgaagcgc cgggcgagcg ccgtcagcgc
     2281 gtcgctcgcc cacggctcgg tgagaacgcc gtccaccacc tcgacgacct tcatcaccgg
     2341 caccggattg aagaagtgat agccggcgac ccggccggga cgcttgcacg ccgccgccat
     2401 cgcggtcacc gacagcgagg acgtgttcga tgcgaggatg cagtcgccct tgacggcctg
     2461 ctcgaggctc gaaaacagct tctgcttcgc gcccaggtcc tcgacgatgg cttcgacgac
     2521 gacgtggcac ggagcgaggc cttccaccga atcgatgatc tcgatgcggc cgacgctcgt
     2581 atccgcctcc gcggcacgga gccggccctt ttccaccagc ctggcgagcg cctgcgctat
     2641 cgagtccttc gccctgcgcg cggcgcccgg ctgggcgtcg aacagcaggg tgcgcgcgcc
     2701 gcactgcgcc aggacctgcg cgatgccccg gcccatggtg ccggcgccgg cgacgccggc
     2761 cacgagatcg ctgcggttgg gatctagcaa gcccttattc taggccgggg gtgacggcta
     2821 taatcggcgc gaggaggggg cagcatgaaa cggatattca gcctcgcagc ggggttgctg
     2881 cttgcgttcg ccgcgcaggc gcagcagttc ccgaccaagc cggtcacgct gatcgtccct
     2941 tggcccgcgg gcggcagcag cgacatctat ttccgcaggc tcggcgaggt cgccgcgcgc
     3001 cacctcggcc agccgctggt gatcgagaac cggcccggcg gcagcgggat gaacggcccc
     3061 gcgaccatga cgaagacggc gaagcccgac ggctatacga tctcgcagct cgtcattacg
     3121 gcgttccgca tgccgcacat gcagaaagtc gactgggacc cgatcaacga cttcacctac
     3181 atcatcggcc ttgccggcta caccttcggc gtcgtcgtca gggccgattc gcccttcaag
     3241 acgttcaacg atctgatcgc ctacgcgcgc gccaaccccg gcaagctttc ctacgccacg
     3301 cccggcacgg gcacgtcgct gcatctcgcg atggaggaga tcggcgccaa ggcgaacgtg
     3361 cagttcctcc acgtgccgtt caagggctat ggcgatggcg cgatcgcgtt gatgggcggc
     3421 catgtcatgg tgcaggtcga ttccaccggg tgggccaagc aggtggatac gggcgctcag
     3481 cgtctgctcg ccaccctcgg cgacaaacgc acgcgctgga acgcgcccac ggtgaaagag
     3541 ctcggcgtcg acacagtatc gaactcgcca tacggcctgg tgggacccaa gggcatgccg
     3601 cgcgaagtgg tgaaagtgct gcacgacgcg ttcaagaagt cgctcgacga tcccgagtac
     3661 ctcaagctgc tggcgatgct ggaccaaccc gcgtggtaca tgtcgagcga ggactacgtc
     3721 cgctgggcgg ccgacatgct aaaggccgag cgtgcgacca tcgagcgcgt aggattgctt
     3781 ctcaaatagg gagggcgcca tgaagtatcg aaagatcctt ctcgcgtaca acggcaccaa
     3841 ggaaggcaaa cgtgcgctct tggaatgcgc cgaccttgcg agcttcctcc aggcggagac
     3901 gcacctgctt gccgtcgcga gcatgccgcc gagcctgttc ctgaccgagg gcttcgtgcc
     3961 cgaagagctc ctggaggagg agaaaaagcg cacccagtcg gtgctcgacg agggcatccg
     4021 caccctgcgc gaccgcggct tcaacgccac cggccagctc gccgttggcg agccggtgga
     4081 ggagatctgc cgcctcgcaa agtcgctcgg cgtcgacctg atcgtcctcg gccacaacca
     4141 gaacacgtcg ttcgccgcgc gctggtggaa aggctcggtc ggcgcttccc tgctcgacta
     4201 ttcaccctgc agcctgctga tcgcaatgtc caagggttga tttaatacaa ttcgggcatg
     4261 ctaaaaggca ggctcgaaga tgatcgcatg ctcaccggtc ggggacggta tgtatccgac
     4321 tggaacctcc ccaaccaggc gtacgcgcat ttcctgcgct ccgaccgcgc gcatgccgag
     4381 atcgtttcaa tccagaaagg cgatgctcta aagagcaaag gcgtcatcgc ggttttcacc
     4441 ggcgacgacc tcgatctgaa atcgctgccg gcggcgcttc cggccaaggg ccgcggcggc
     4501 acggagctca tcaacccggg caggccggcg ctggcgaaag gtcgggtgcg tttcgccggc
     4561 gacgcggtgg cgctgatcgt cgccgagagc gcggttgccg cacaggacgc tgcggagctg
     4621 atcgctgtcg aataccgtga tctgcccgcg gtcgtgaaga ccggggatgc gctcgccgcg
     4681 ggcgcgcccc tcgtgcacga ctcggtgccg ggcaacatgg tgctggatta cgagtcgggc
     4741 gacgaagcgg gggcccgcgc agctttcgaa agcgcggcgc gcatcgtcga gctgacggtg
     4801 gacatttcgc gcgtcgtcgg caacccgatg gagccgcgcg cctgcctcgc cgcattcgtc
     4861 tccgaccaat accacctcta cacgtgcacg cagggcgtcg ccatcatgcg caaccagctt
     4921 tccgcggtgc tcggcgtgcc gccggagcag atccgggtga tcgcggaaga ggtcggcggc
     4981 gggttcggcg tgcgcttcaa tctgtacccg gaatactgca cggcgctgtt cgcggcgaag
     5041 aagctggggc ggccggtgaa gtggaccggg acgcgctcgg aagtgtttct cgccgacgag
     5101 caggcgcgcg acgtgcgctc gcgaggagag cttgcgctcg acggccaggg acgcatcctc
     5161 ggcatgcgct tcgacatggt cgccaacctc ggcgcctacc tcgcgccgac cggcccgttc
     5221 atcaacacca tcggcatcgt caactgccta tccggtgtct acgacgtccc ggcgacctac
     5281 gcccgcatcc ggctcgcgct caccaacacc gccccgatgg cggcctaccg cggcgccggg
     5341 cggccgatca tgtcctacgc gatcgagcgg ctgatcgacc acgccgcgcg cgagctgggc
     5401 ctcgacccga ccgagctgcg gcgccggaat ttcatcccga agcacaagtt tccgtacaag
     5461 atcgccgccg gcttcgaata cgactgcggc gactttgccg gtgtgctcga caaggcgctt
     5521 caggcttctt cctgggattc cttcgaccaa aggaagaccg aggcaaaaag gcgaggcagg
     5581 ctgcgcgggc gcggcatcgc tacctacatt gaagccaccg gcgcgggctt cgcgccgcac
     5641 gaccaggtcg agctgcgctg ggacgaggga ggatttgatt ccggcggccg catcaccgtc
     5701 tacgccccga cgcacaacca cggtcagggg cacgagacca cctacgccca gctcctgtcg
     5761 ggcgtgctcg gcctgccgct ggaggcgttc cgcctgcgca ccgcgggccc cgagttctac
     5821 atcgccggca acgccaccgg aggctcgcgc tcgctgctcg cggtcggcag cgtggtgatg
     5881 ctcgccgggc acgagatggt gaagaagggg atggcgctcg ccgccgacga actcgaggcc
     5941 gcagtcgcgg acctcgagtt cgtgcagggc aactaccgga tcaaggggac agacaggaag
     6001 atttcaatac agtcgctggc gcggcaacag cccggcaacc tcgacatcca ataccagaac
     6061 aagttcggtg cgaccttccc gaacggttgc cacatcgccg aggtggagat cgagcccgag
     6121 accggagacg ccgagatcgt gtcgtacgtc gcctgcgacg acgccggcaa catcatcaac
     6181 caccagatcg tcgaggggca ggtgcagggc ggcctgaccc agggcgcggg acaggtgttc
     6241 ggcgagctcg cggtctacga ccgcagcggc cagttgctca cgggtagctt catggactac
     6301 gccatgccgc gcgccggcct ggtgaacggc atcaagctcc tcgaccaccc ggtgccgacg
     6361 aagctcaatc cgctcggcgc caagggcgtc ggcgaggcgg gcgtcaccgg ctcgttgccg
     6421 gcgctgatga acgccgtggt cgacgcgctg cgccaggcgg gcgtgcgcga gttcgaaatg
     6481 ccggcgacgc cggccagaat ctggaaagct attcaagaag caaagaacta ggcaagtttc
     6541 ttgcaacaag tgcggcgtga gctccgatgg gacagacgag ccatcgtccc cggttagacc
     6601 ctcgagctgg tccgcagcgc tttcccagct tcctggctat ccccgtgacg agaagccgcg
     6661 gccgccggta gctgatgaag cgcctcgcac ggtgacgccc atgaaatccg gcgacgaccg
     6721 ctagaatcgg ggaatgcggc gcagcagcga gcgcattctc accacccacg tcggcagcct
     6781 catccgcccg cagtcgctgc tgccgcttat cggcgcgaag cagagcggcc agccctacga
     6841 tcgagagctc tactcgaagc gcttggccga atcggtcgcc gaggtggtcg cgcaacaggc
     6901 cgccgccggg atcgacgtcc caagcgacgg cgaattcggc aagtcgatca gctggtcgca
     6961 gtacgcgctg gagcggctga gcgggttcga gcgcaggccg gtggccgcgg gcgcaagccg
     7021 cttcgcgcgc ggcgccgatc gcacgcgctt tgccgagttc tacgtcgagc tcgacgcggc
     7081 gagcggcccg cccgcgaccg ccggcgcgag cgccggggaa gcggtgtgcg tggcgccgat
     7141 ccgctacacc ggccaggcgg aggtgcagcg cgacatcgcc aatttcaagg cggcgcttgc
     7201 gcgcgccggc gtcgccgagg gtttcctgcc ggtcgccgcg ccggcgagcg tgatccccga
     7261 tcgcaggaac gagcactacc cgagcgacga gaagatgctc gaggcgatcg ccgacgcaat
     7321 gcgcaccgag taccgcgcga tcgtcgacgc cgggttgctg ctgcagctcg acgacgcgcg
     7381 cgcggcggtg acttacgacc ggatggtgcc gccggcaagc ttcgccgact accggagctg
     7441 gctcgagcgc caggtggagg cgatcaaccg cgccaccgag gggattccca gcgatcgcat
     7501 ccgctaccac gtctgctggg ggagctggcc gggcccgcac acgagcgacg tgccgctcaa
     7561 ggacatcgtc gacctggtgc tgcgcgtgcg cgctggcgcc tatctcatcg aggccgcaaa
     7621 cccgcgccac gagcacgagt ggcgggtatg ggaaaaagcg aggctccccg agggagcggt
     7681 gctggttccg ggggtcatca gccatgccac gaacgtggtg gagcatccgg agctggtcgc
     7741 cgagcgcatc gtgcgcttcg cgcgcctggt cgggcgcgag aacgtcatcg cgggcaccga
     7801 ttgcggcttc gcgcaggggc cgttccatcg cagggtgcat ccgtcgatca tgtgggcgaa
     7861 gctcgaagcg ctcgccgagg gcgcgcggct ggcgagccgc gagctctgga actaggcggc
     7921 cctgaggtcg cgccagcgct tgccgtagcc gcgctcgagg ctgtccaggc gcgcacgcag
     7981 cgcgcccagc gctctgtcga tctcgccccg cagggtgccg acacgccggt ggtcgcgctc
     8041 ggaatacagg tccttggcgc attgcacgag gagatcgatc tgctcctggc actcgccgtc
     8101 gtcggctagg agcatcgcct cccagcacag cccgcggaat tcgagcagcg actcgtcgtc
     8161 gtcggcgccg ccgtgagact tggcgagcac cgctcccatc ccggcgagag ttcctcggac
     8221 tttctgcaga tcggcgtgct tcatgcgtac gcgcatgttt ccaccggtac gacggctagc
     8281 acaatccgat tcacagggtt ttacggccgc aagtgtgacg tatttcacgg tcgcagaccc
     8341 aaattgacgc cgcccaagtc aggcgacgcg gcgctcggca tttccggcga tgctgcctgc
     8401 gccgatgaag ggctcccgcc gcccgtcgag cgattccggg tggtcttcgc ggcggtagtc
     8461 gtgcgtgcgc cgcaaccggt cttcgtactg ctgccgctgc gcgtggaaca ccgctttccg
     8521 ccgcatcgcg gcaagcacgc cgagcgccat gaccgggaga ccgacgagcg cgaagggcag
     8581 cccgacgtgc acccgagtga ggatccaggt gaccgcgcta tgcacgggca gccaagcgtc
     8641 gggattcatg agccacggcc aggggacttc cgggatgaat tcaagcaccg gcgccgcctt
     8701 tgcggcctgg aggagcgagt ggtcgctgaa gagcagggtg gcgggaagcg caatccacga
     8761 cctgatctgg atggacgccg ttatctgcca ggcgaacagc gccacgaagt acagcacgag
     8821 cgccaggccg gcgacgacga gcgcaacacc gaatacaggg actttccttg ctttcatgca
     8881 tgcactccag gttagccccc tacccagggc catagcatgc cctgcgcaag cgaagtgcgc
     8941 tggcgccggc gctagctcgg aaggactatt tacggctggc gcgaatgcga ggcgaagaaa
     9001 tcgcagacca gcttcgtggc atcgagatgg cggctgtcga agaacggata cgccgggtcg
     9061 ccgccgctcc acgcgtgccc gagcccggga acggtgagga gctgtgccgc gagccgccgg
     9121 ccggcgtggt actcgctgag aaggtagtcc gcccggggct ggaaggcgcg gatctgcgcc
     9181 gggggcagcg cggcgcccgc cggcagctcc tgcggagcga tgccgttgaa gatcaggaac
     9241 tgccgcacca ggaacgccgc gttcaccggc gcgacggtgt cgtcggccga cccgtggatg
     9301 acgagcagcg ggacacgcgc cttcccggct gtgcgggcac gcgcttcggc gccgagcgca
     9361 tcggtccccc cgaacgggcc tttggccatg acgcgcgccg cgtccgcggc gtccgaggcc
     9421 gcgccgcagg cgacggcaga gtgcaccgcg accgcggcaa agagatgcgg cgcgcgcaat
     9481 gcgagggtcg ccgccagcgt gccgcccgac gacaggccgg cgacgtacac gcgctcgggg
     9541 tgcaggcgga acttctccac cacctcggcc acctgggccg cgacgatcgc cgcctcgcct
     9601 gcgccgcccg cggtgcgccg gtcgaaccag ttccagcagc gctccgaatt cgcgagacgg
     9661 ctctggcgcg gcaggagcac cacgaatccc cgctcgtcgg cgaagcgggc gatgcgcgtg
     9721 ccggcggcga acgcttcggg atcctgccgg cagccgtgga tccacaccac gagcgagcgg
     9781 cgctcgcgcg cgtcgaagtt ccgcggcaga tacaggaggt attcgcgcca cggcggcgcg
     9841 ggcgcggcac caatgaaccc gtgcgcgctc gacttgcggc tcagggagaa ccgcccggga
     9901 gcgggcggcg cccggcgcca cagccggtgc gccagcccgg cgatctttcg cgtcagcgtc
     9961 gtgatggaca cgaggataca atcgcatgag ggactaaagg ggagggcaag tgagaacttt
    10021 actgggtatc gcaactgcag cgctgctcct cgttcctgct gcggcgcagg ctgcggccga
    10081 gttcaagatc gccaccgcgg acaagcgcgg cacctacttt gcgatcggtg ccgatctcgc
    10141 caagttcgtc gcgcccggcg ccgacatttc gctggaagtc atgcccaccg acggctcggc
    10201 ggcgaatatc cgccaccttc gcttcgatgc cggggtgaag ttcgcgatcg tgcaggccga
    10261 cgtgtaccag gctttcgtgg atcgcggcgg cgcaaaccgc gacgcggcca gaatcatcgg
    10321 cccgctgcgc gtcatcctgc ccctctacaa caccgagatc cactacatcg cgcgcgccga
    10381 ctcgccgctc aactacctgc acgatctcaa ggacgcgaag atcaacggcg gcctggtggg
    10441 aagtggcgcc gccttcatca cccacaccct gtaccggatg atgttcaaca gcgcgatgcc
    10501 ggaggccaac gcgagctaca tgccgaacga cgaggcgctg atcaagctga tcacggacaa
    10561 gtcgatcgac gtggcggtag tcgcggccgg acaacccgcg ccgatcatcg ccaacatgaa
    10621 gcccgaggcg cagaagtaca tcaagctgct caagttcgat ccctcgcacc cggcgagccg
    10681 cagcgcgctc agcatctacg cgccggcgac cgtgcatgcc aagagctatc cgaacctgct
    10741 cgccagcgac ttcaccacga tctcggtggg cgcgtttctc gtcacctacg actacaacct
    10801 gcagacgacc gtcgactatc tcgcgcgatt cgcccgctcg ctgtgccaga acttcgcgac
    10861 gctgcaggcg aagggccatc cgaaatggcg ggaggtcgac ctcactctgc cggcgctcaa
    10921 caagggatgg acctactacc cgccgacaag cagagagata cgcaactgcc tcgccgatcg
    10981 gtcgaaagcc ggccgggcaa aggcatgctc cgccgaggag cgcatcctcg ggttctgcaa
    11041 ctgagcgttt atcccgaagt ggcgtgctga ctgctgcggt gcactcaccg ttaatccccg
    11101 cggcgtgcca gaatccggat aggtaacggc agcatcaggc aggggagggg gggatttctt
    11161 gaagcgcaca cagccgcttg ggccggccgt gcggatcctg ttgctcgaag acgacccgat
    11221 cagcgtagag atcgtcggta cgtatcttcg gcgcattaca t
//
LOCUS       DATBSZ010000136        43403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_497280, whole genome shotgun sequence.
ACCESSION   DATBSZ010000136 DATBSZ010000000
VERSION     DATBSZ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 43403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43403
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497280"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3066
                     /locus_tag="VIG70_08695"
     CDS             <1..3066
                     /locus_tag="VIG70_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY8250671.1"
                     /translation="HEQRLAALAREAGFTQVSLSHEVSPLMKLVSRGDTTVADAYLSP
                     LLRRYVERIAAELPDVRLMFMQSNGGLAGARQFQGKDAILSGPAGGIVGAVRTSAAAG
                     FDRIIGFDMGGTSTDVSHYAGELEREFETRVAGVRMRAPMMSIHTVAAGGGSILHFDG
                     ARYRVGPDSAGANPGPACYRRNGPLTVTDANVMLGKVQPKYFPRVFGCEGNEQLDAAI
                     VQEEFRKLVSKIKDGRSAERVAEGFVQIAVGNMANAIKHISVQRGHDITRYTLCCFGG
                     AAGQHACLVADALAMTRVFIHPHAGVLSAYGMGLADQVAIRSRAVEARLEGCDLEDAA
                     EALAIEARNELVAQGLPPDKIAVERKAHLKYEGTDTALVVRLGPAPAMLREFEAAYRK
                     QFSFLMPGRPLIAEAVSVEAIARGEVFREEALPSKEKAKPAEIVRMFTGGRWHDAPLY
                     RREDLGPGNRVDGPAIVAEANATTVVEPQWQASVTALNHMILERTTEKKRSSAIGTSV
                     DPVMLEIFNNLYMSIAEQMGLRLQNTAYSVNIKERLDFSCALFDADGNLIANAPHMPV
                     HLGSMGESIKTVIRLNAGKMKPGDVFVLNAPYNGGTHLPDVTVITPVFDSNGKEILFY
                     VGSRGHHADIGGITPGSMPPGSKVVEEEGVLIDNFLLVEQGRFREKETVALLTSGKHP
                     VRNVQQNIADLRAQIAANEKGVQELRRMVEQFGLDVVRAYMRHVQDNAEESVRRVIGV
                     LKDGDFDYPLDNGARIKVRIQIGKDKRSAKIDFTGTSAQLPNNFNAPAAVCMAAVLYV
                     FRTLVDDEIPLNAGCLKPLEVVIPEGCMLRPRYPAAVVAGNVETSQCITDALYGALGV
                     LAASQGTMNNFTFGNERYQYYETVAGGSGAGPDFDGADVVQTHMTNSRLTDPEVLEWR
                     FPVRLESFGIRHGSGGGGRRRGGHGAVRRVRFLEPMTAAILSGHRLVQPHGLAGGEAG
                     AAGRNYVLRAGGGVSELGPFEQTEMAAGDVFVIETPGGGGYGAVKSGKE"
     gene            3080..5881
                     /locus_tag="VIG70_08700"
     CDS             3080..5881
                     /locus_tag="VIG70_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY8250672.1"
                     /translation="MNASAIYSKSGKGVQEASGKTNVLQRSDRAVLAAIDGRATLADV
                     AQKVGKPFDPAFEKLIESLDKGGFIREVSAGAPGVAGVTTKAPAAGGKPATKSAPVDP
                     SSDLDFTTALSIPKGKPAPPPPKAAPPPSAAEKAQAAKSAVEQEDAFAKAREEAERKK
                     QADRDRLKAEAEAKMREETEAKLRAETAARLKAEAEAKAKADAEAKIKAARDAAAKAA
                     AEAKAKAEAEAKRAREEAERARKEAAEVAERARKEAAEVAERARKEAEAKAERARKEA
                     EEKARHEQEELKRKLEEERTAREEAERRAQEEAKRLEEERRKLEEERKRHAEERRREE
                     EERTLRRIQEERERVDRKRDEAARKKRREEEEAQEEEKPRTKQKEPEPDPEPTAPPPV
                     AAAGGAFSDTLLADLDSFTTRDDQDQKDKEEAARKEQEAAAVRAREAAALRAKEDAER
                     QDREAKQRRKQEEAERKALEQAELRAAEEREKERLREEEEKRKQEAEERERKAKQEEQ
                     VTAQSTRSGAMFDAEMRAKRERELMARAPRAAAGTRAEKKGWGKPIALTLFLLLVVAL
                     GVAHVLPIDTAEYERAASDALGRPVKIGSARLSLITGLQIRFSEVNLGEGVSMSEVRA
                     FPEFDALTGPRKAFSRIEADRMKIEQDMVGQALFAKVKADNFSVGQVVMKNVEFTGPL
                     VLPKGLELELVYDGAGMVRSAAVRGPEALSAKLTPKGSSTEVEMTAASFTLPFAPEIA
                     LSKFSMKGTATPQGLSVTEWDGALLNGNVSGTASVRWGESWTVEGVVTARNINAAVFA
                     PALLSDGKAEGSGKFSMRGPPGKLTATSRLDGSFTVTRGVLGSIDLSRAIQTGGKVAT
                     GRTQFNEMNGQVTYDRGAVSLRNITIGAGQLNAGASADIAQNGALSGRIVADVRVAQQ
                     RLSATLNLGGTLSEPQVKN"
     gene            5895..9740
                     /gene="purL"
                     /locus_tag="VIG70_08705"
                     /gene_synonym="purI"
     CDS             5895..9740
                     /gene="purL"
                     /locus_tag="VIG70_08705"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEY8250673.1"
                     /translation="MQSITQLRGPRALSESRLAKLLASLQKLDPGVRSASTEYRYFVE
                     TAGELGVPGQRLLERLLDDGSPRPQTASGALYLVVPRLGTLSPWSSKASDIARNCGLE
                     QVRRIERGTAFHIEGAKTDPSPLLHDRMTQTVLRSFDQAAKLFEHVPPRPLQHVPDIR
                     RANRDLGLALSEDEIEYLERAYRRLGRDPTDVELTMFAQANSEHCRHKIFNADWIIDG
                     ARQPQSLFAMIRHTHAASPQGTVLAYSDNAAILEGRPASRFFPDEKQVYGRNSELTHI
                     VIKCETHNHPTAISPFPGAATGAGGEIRDEGSTGRGAKPKAGLVGFSVSHLRIPGHER
                     PWERDGVGKPDRIASALDIMLDGPIGAAAFNNEFGRPNLCGYFRTFEFQNRGYHKPIM
                     LAGGVGNIAAGHAHKQPVPPGSLFIQLGGPGLAIGMGGGAASSMGAGANVEDLDFDSV
                     QRDNAEMQRRAQEVIDRCWQLGAANPVLSIHDVGAGGLSNALPELAHGAGRGARLELR
                     AIPSEDSGMSPREIWCNEAQERYVLAISKQDLEAFKKICERERCPFAVVGTATDDGRL
                     VVEDSRLKQTPVDVELEMILGKPPKMLRDVKRAHPRPAAISLNEITLEAAVSRVLQHP
                     AVADKTFLVSIGDRTVGGLCARDPFVGPWQVPVADCAVTLLDYEGYAGEAFAVGERTP
                     LALIDAPASGRMAVAEALTNLAACGASLSTTKLSANWMAAAGYPGEDAALYDTVRAVA
                     LELCPALGISIPVGKDSLSMRTAWGDKEVVAPMSLVVSAFAPIADARAALTPQLRTDA
                     GDTELVLVDLGRGKNRLGGSILAQVFSQLGDDCPDLDDPALLKGFFSFVSENREQLLA
                     YHDRSDGGLFVTACEMAFAGRCGVTLSLDAIAFAAAADDVDAFKRDAGEQLAGRAKDL
                     ALQALFSEELGAVLQIRASDRARVMAALRAAGLGEHSHVIGSLNPRDELRITRNNRVV
                     FAAKRAELRRTWSETTWRMQTLRDNPECAREEYDRILETDDPGLSVLLAHEAKNIAIS
                     TGTRPRIAILREQGVNGQVEMAAAFDRAGFESVDVHMTDLIAGRASLEGFKGFTAGGG
                     FSYGDVLGGGQGWAKTILFNARAREEFERFFARQDTFALGACNGCQMMGALRELVPGA
                     SGWPLFVRNRSEQFEARLVMVEIVESPSIVFAGMAGSRIPIVTAHGEGRPNTENPSIV
                     CMRYVNNRGEPTQTYPYNPSGSPGGITGLTTPDGRFTIVMPHPERAFRNVQMSWSPPG
                     SGEDSPWMQLFRNARKWVG"
     gene            9747..10976
                     /locus_tag="VIG70_08710"
     CDS             9747..10976
                     /locus_tag="VIG70_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HEY8250674.1"
                     /translation="MQRSDVLIVGAGIGGLTLALELARRRIPCRVFESAPEIKAVGVG
                     INLLPHATQVLGELGLEPALARVAVETAESVFFNRFGQLIYKEPLGRKAGYPWPQFSI
                     HRGDLQALLLDAVKAKIGAERIHLGWHCLGFEQDASKVTLRFRDRAPMDGTIAVGCDG
                     IHSVIRKQLYPAEGEPRYSGVNMWRGVTRWKPILSGASMLRAGWLANGKLVHYPIRND
                     VDETADGTGRQLVNWLWEIETPQYKRWDWNRPARVEDFIAGAESWRFDWLDVPAFLRA
                     SELVLEYPMVDKDPLPAWSFGRVTLLGDAAHPMVPRGSNGAGQAILDARALAQCVSEE
                     KNSLKGYESERLGPTSNVVLENRRNPPDAILREVYERTGDRPFERIDAVISAQELAAL
                     SDRYKQVAGYDKERLQR"
     gene            10973..11512
                     /locus_tag="VIG70_08715"
     CDS             10973..11512
                     /locus_tag="VIG70_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250675.1"
                     /translation="MSFLHRLFPAGHFVVSVLFLVAGAALIVIAGMQLWQGIQPFEAA
                     AISQRLNAVLESIAVLTVAVAALELGQTILEEEVHRGAQMSAPTRVRRFLSRFMVVLV
                     VALSLETMVLVFRVSQEAPEQLPYVASVGAAAALLLIAWGVFIRLNRSAEELEPEAMQ
                     AAKREDRKIEKSAKGEAKS"
     gene            11509..11856
                     /locus_tag="VIG70_08720"
     CDS             11509..11856
                     /locus_tag="VIG70_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8250676.1"
                     /translation="MSITRHDPTSILSNAVQHGDTVYLAGVVAKNLDQDVKGQTKQIV
                     DEIDRLLAKCGSSKSKVLSATIWVTDIRNRAPMNEVWTAWVDAKNLPARACVEAKLAD
                     PKALVEIMVIAAR"
     gene            11930..12151
                     /locus_tag="VIG70_08725"
     CDS             11930..12151
                     /locus_tag="VIG70_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250677.1"
                     /translation="MLINIGDIAAECPQCGGADFETLSGGPLRLATELRCTGCGEHVK
                     YLLLLDHIGEEAMRRANRSLDELKKQPKK"
     gene            12252..12629
                     /locus_tag="VIG70_08730"
     CDS             12252..12629
                     /locus_tag="VIG70_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250678.1"
                     /translation="MLVIEKGAEAGLICAALARSPDVRVIDAPDVADALERLDRDPAP
                     VALAIAGEMTLSADAGELVRRLGGRGIPVVGVAPGLPAAARQRALAAGVREIHDRPAE
                     WRPYSALIDSLVARFTPAGLPPR"
     gene            12642..13070
                     /locus_tag="VIG70_08735"
     CDS             12642..13070
                     /locus_tag="VIG70_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8250679.1"
                     /translation="MNTLVLPRSWLILASIVVFFAIATRSSDPRVGDSLVTAQRINDV
                     SIAEAIALIDAGALVIDVREGPKTHLPGAMLIPLEVLAAHLSKLEGAKAQSIVVYCGN
                     GTSRGPRAAHILTQAGFTHVVNLEPGIEGWRAAKLPVVAS"
     gene            complement(13072..13902)
                     /gene="serB"
                     /locus_tag="VIG70_08740"
     CDS             complement(13072..13902)
                     /gene="serB"
                     /locus_tag="VIG70_08740"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEY8250680.1"
                     /translation="MNLVVQGPALGKADVDALAAMASPSGVDGITARAFRLRSARKDA
                     AIPSYCETHHIDHAWVPEGRRFADLKLLAMDMDSTLITIECIDELGDFAGKKAEIAAI
                     TAQAMRGEIEYRESLRRRVAALAGLPETSLNEVYEERLKLTAGADTLIAACRKHGVKL
                     LLVSGGFTFFTERLKSRLGIDYTISNVLEVKGGKLTGALIGEIVDADAKAKKFLQVAT
                     GLGARKEHTVAIGDGANDLKMMNEAGLSVAFHAKPVVRAQASCALNWTSLDGVANLFE
                     "
     gene            complement(13899..17318)
                     /gene="mfd"
                     /locus_tag="VIG70_08745"
     CDS             complement(13899..17318)
                     /gene="mfd"
                     /locus_tag="VIG70_08745"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY8250681.1"
                     /translation="MHRDSLPARRRYTRLPGSADALALGRLAQEEMPRSAPLALITAT
                     ALDAQRLLEEIAWFSPQLRVCLLPDWETLPYDQFSPHQDLVSERLSTLYRIQRGDFDV
                     AIVPAATALVRLCPPSYLAAHTFFLKTADRLSIEALRQQLAVAGYQHVTQVVSPGEVC
                     FRGGLIDLFPMGSALPYRLDLDDDVIEAIKTFDVDTQRTLYGVKEIRMLPAREFPLDD
                     AGRARFRSRWRELFEGDPSKKRLYRDVSNGVPAAGIEYYLPLFFESVATVFDYFPAGS
                     TLALHHDVSGAIQEFWRDANSRYKMAGGDPDRPLLAPAQLFLPAEEFFVRAQAFARVD
                     VLDKPDEDQGPVSAVPLPALAVDRRAQDPLAGLKHFLETSGLRVMIAAESPGRRETMA
                     SYFAEYGLKPDLCTSFEDFVQKSSKCMLGVSPLSHGFLLPAEGWAVVTEAELYAGVVR
                     RRGRAAEKRSSVEGMLRDLSELKVGEPVVHEQHGIGRYQGLVTLDVGDGKTEFLLLQY
                     EGDDKLYVPVSQLGVIGRYSGAQPEEAPLHKLGSGQWDKAKARAAKQVRDTAAELLAL
                     YAKRAARHGHAFGIRQHDLEAFADGFGFEETPDQASAIESVIADMAAGKPMDRLVCGD
                     VGFGKTEVALRATYVAVADSKQVVILCPTTLLAEQHFQTFSDRFADWPVRIAELSRFR
                     STKESGEIIKKLENGEIDVVIGTHKLLSRDVKLKRLGLVIIDEEHRFGVRHKEALKKL
                     RAEVDVLTLTATPIPRTLAMSLEGIRDFSVIATAPEKRLAIKTFVAPWSEGLIREAVM
                     RELRRGGQVYFLHNEVDTIERMRERLAKLMPEARIAVAHGQMRERDLERVMRDFTAQR
                     FNVLLCSTIIESGIDIPTANTMVINRADRFGLAQLHQLRGRVGRSHHQAYAYLLTPPE
                     EALNKNAVKRLEAIQMMEELGSGFYLAMHDLEIRGAGEVLGEQQSGEIQEVGFSLYAR
                     MLERAVRRLKAGKAPEADDAVDVSTEVNLHVPALLPETYCSDVHERLSLYKRLADADT
                     REQLDTLREELVDRFGELPDPARALIECHRVRIAARPLGIARVDATHEAVQLQFIKNP
                     PLDGAKVIEFIRKKGRSARLAGPEKLRVEAKLPAWQERAQAVKDILSQLAA"
     gene            17382..18077
                     /gene="ispD"
                     /locus_tag="VIG70_08750"
     CDS             17382..18077
                     /gene="ispD"
                     /locus_tag="VIG70_08750"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY8250682.1"
                     /translation="MSIYALIPAAGSGTRLPAEGPKQYLSLAGRPMLWHAVRAVCVPP
                     VESVFVVLAPDDKAFASHDWSAFADKLEPLYCGGETRRDSVYNGLVAAMAALDADDWV
                     LVHDAARPCLPREDLESLVRETQNDEVGGILALPVAETVKKVAKDEGGAQRISGTEDR
                     SQLWLAQTPQMFRAGLLMRALQRAKGTPTDEAAAVEQLGLRPRLVAGSRENLKVTWHG
                     DLAIAESILKARA"
     gene            18074..18541
                     /gene="ispF"
                     /locus_tag="VIG70_08755"
     CDS             18074..18541
                     /gene="ispF"
                     /locus_tag="VIG70_08755"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY8250683.1"
                     /translation="MRIGQGFDVHPLVSGRKLIVGGVEIAHPKGLEGHSDADVLLHAI
                     CDALLGAAGLGDIGRHYPDTDPAYAGADSRALLRDVAQKLREKKLKVVNLDATILAQA
                     PRMAPHMPRMIANIAADLGIAPAAVNLKATTTEGLGFVGRLEGIAAQAVALVD"
     gene            18543..19160
                     /locus_tag="VIG70_08760"
     CDS             18543..19160
                     /locus_tag="VIG70_08760"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEY8250684.1"
                     /translation="MYSPSYNRLEDRAELLAFLQANSFALLVTGTGGTLHASHLPVMV
                     KEHEGKISLDMHMARNNPQWQEFFDDEVMVVFSGPHAYVSPRWYEEQERVPTWNYAAV
                     HAYGVPRLIDEKKLKHENQRRLVAMLDPQWLPRFDALRPEYVEHMLAGIVNFEIAVTR
                     LDTRWKLSQNRSRREQELIAAELEKSPDPAERALAELTRKHLTGN"
     gene            19157..20365
                     /locus_tag="VIG70_08765"
     CDS             19157..20365
                     /locus_tag="VIG70_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8250685.1"
                     /translation="MKVVVMGAGLAGVTTAWALARDGHEVVVIDREPEVAAGASYANT
                     GIVAASRAYPWSSGEIGRALRRSALRLDPELLVWGIQHLLLRSGGSYRRVLEAKVRLV
                     RYSQQVMHEMVREIAFDGLKRGVLYLYREAQALDEAWVRARSMRDLGFALERFEPAQV
                     FHHEPAIAASRLAGALFAPGDEWADAALFCRSLAAKCRPLGVNFSLENEILSIEPADA
                     RVREVVTRRGRVRGDAFVCALGVMDRKLREQLGTHVPVYPVKGYSATVPILRSDAAPR
                     RAMIDESRGIAFSPMGADLRITGGAEFAGYGKEHEPHDVALLYDTVKQLFPGALDYAR
                     ARVRACQRPMTPESTPRFGTGKYQNLWFNIGLGHMGFAMAAGAARITAELVSGRTAPI
                     DLEGLRIRRH"
     gene            20365..20865
                     /gene="thpR"
                     /locus_tag="VIG70_08770"
     CDS             20365..20865
                     /gene="thpR"
                     /locus_tag="VIG70_08770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY8250686.1"
                     /translation="MRIFFAVWPPAETAHALAQWAREAQRGTGGKPTDEAKIHLTLAF
                     LGDADAQRAIGAARRVTANTHGLPIEQASYWRHNQIVWVGPRETPPPLQALFDRLSEE
                     LYREEFILERRPFAAHVTLVRKARAAALPPLAALDWPVREFLLVRSSLSSKGSTYEPL
                     ERFALS"
     gene            complement(20862..22139)
                     /locus_tag="VIG70_08775"
     CDS             complement(20862..22139)
                     /locus_tag="VIG70_08775"
                     /inference="COORDINATES: protein motif:HMM:NF027838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8250687.1"
                     /translation="MTLFARSFLLIALLIVTAVLASFQIYRLYEREPRSRELAQQTVS
                     TVNLTRAALVSADPFLRRELLIELNETEGLRVFPATASEKLEPVPDEPLLNLVLRKVR
                     AVTGAETRFAYARDGDEGFLVSFFIDEDEFWVMLPRERFELEFGLEWLGWGAALLALA
                     LAGAWLIASSIARPLAALTRAAARIGRGEAHQPLAEEGARELRSLGAAFNRMARDLES
                     MERERAMVLAGISHDLRTPLSRMRLALEMSGAEAGAADTIVADIDEIDAIIGQFLDFA
                     RGVNEEQSRQDLGTMLDEVAEHYTRLGKAVHLKKEEVAPFRFARTAIRRAVSNLVDNA
                     LRYAGEPVEIELKKNPGSAVIEVRDRGPGIPAAETERLKRPFTRLDAARSGPAGAGLG
                     LAIVERVARAHGGALELAPREGGGLVARLALRA"
     gene            complement(22140..22856)
                     /gene="ompR"
                     /locus_tag="VIG70_08780"
     CDS             complement(22140..22856)
                     /gene="ompR"
                     /locus_tag="VIG70_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEY8250688.1"
                     /translation="MPELKTRILIVDDDQRHRDLLVKYLSGEGYDVKAVPDAGAMDKQ
                     LARDRYDLVVLDLMLPGEDGLSICRRLRSQQSAPAIIMLTAKGDDVDRIVGLEMGADD
                     YLPKPFNPRELLARIVAVLRRRAPAGPPGAPAEGGVFAFGEFSLNLATRTLTRGGKQV
                     ALTTGEFSVLKVLVQHPRQPLSRDKLMELARGREYEVFDRSIDVQISRLRKIVEQDPS
                     HPKHIQTVWGFGYVFVPDQA"
     gene            22919..23182
                     /locus_tag="VIG70_08785"
     CDS             22919..23182
                     /locus_tag="VIG70_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250689.1"
                     /translation="MMLRAALALILALSAVDGSAQSGKRHDGGRDRQMSQEERQRMRE
                     DVRDAYRERQDRPQQKARPQQMSPEERGRLRRDIEDANRDLKR"
     gene            complement(23179..25938)
                     /locus_tag="VIG70_08790"
     CDS             complement(23179..25938)
                     /locus_tag="VIG70_08790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8250690.1"
                     /translation="MAKAAKKRAPAARSSARRQPAAAAPAPHFSFLGLTSDWYWEQDA
                     ELRFTRVEVRKDSAPEQGLAQRILGKKRWETGVEIEGGWDAHRAMLEARAPFRDVLMW
                     RNLPDGARRYISVSGEPTFDARGRFTGYRGIGRDITKQKRIQQLLKLDHAVTLRLAEG
                     RGAPQALNGALEAICDSLRWDSSQLWTPDEPAGVLRRISHWSTPGDAAAARFVEASGD
                     LAFAPGEGLVGTVWQAGDPVWVPDATSDPRALRRAIAQETGLRAAAIFPIRAGERIAA
                     VLEFTSRRIRQPDNRLWQTLGAIGTQIGQFLGRAQAERAVRESEARWRALTHLSSDWY
                     WEQDAEFRFTRLEGRQVAGGDPDLQRRLLGQRRWESGLGLEGGWDAHRATLEARKPFH
                     DLLMWRTMSDGSLRYVAVSGEAVFNADGSFAGYRGVGRDVTEEKRAEQILRLEHEVAR
                     LLAAAEDAAGGLRNVIRVVCETEGFACGRYFRADPDLLRFQDAWAIADPAIAPFVERS
                     RTFVFRPGEGLTGTVWLSGEPVWSPDITRDPRVRDRSVWTGTGLRGGFAFAVIAEGRT
                     IGVLNFSSLKAREPDQRLLAASRVIGSQVGQFLQRKQAEASLRESEARFRSLTQMSSD
                     FFWETDEAHRYVQLVYGPGYLPPEMGRGAIGKTPWELPCESPDDAGWAAHRAQLDQHK
                     PFRDFEFARRMPDGLVRYLSVSGDPRFGAQGAFLGYRGVGRDITEIALARERIASLAY
                     SDPLTGLANRTSLMPALDQAVQRARRRNSKIAVVFLDLDGFKAINDAYGHDAGDALLV
                     ELAGRLRDNLRASDFIARLGGDEFLVVLEEVQDQEPVEIVAKKLLVETVRPYTLPGAR
                     ATVTASIGISIFPDDAADATALVKHADIAMYAAKQAGKNTCRFYSSGPAANDPARQAR
                     ESGSS"
     gene            complement(25996..27378)
                     /locus_tag="VIG70_08795"
     CDS             complement(25996..27378)
                     /locus_tag="VIG70_08795"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY8250691.1"
                     /translation="MTELVALPLWLALAVGLFALWALYEHVLIPALRWMLVHPADRVI
                     EDVSTRLRIGIRPFQRTRRQALIHRLLTDPKVQEAAEQFALANKIPIGTAFKKIESYA
                     REIVPAFNAYLYFRTGYWLGRRVARLLYRVRVGYVDGPGLERIDPSATVVCVMNHRSN
                     MDYVLAGFLAADQAALSYAVGEWARIWPLSALIRAMGAYFVRRNSRDELYRRVLERYI
                     AMATEAGVPQAVFPEGGLTRDGRMRELKLGVLDYMLRGFRLAGDRDLVFVPLGLNYDR
                     VLEDRSLLKEKGSTGRAKSLWITFRFLLHNASLMLRSEWHRFGYACVNFGTPISMRER
                     GIDYQALSGEERRAAVAALGIELMQAVGRIVPVVPVPLIAAVFVSQPQRALSELELKA
                     EVGRLLARLQAAGAHVYIPRGDLDYALSVGLRMLRLRRLVEEEQGLFRARQEELPLLA
                     YYANSIAHLI"
     gene            complement(27375..28244)
                     /locus_tag="VIG70_08800"
     CDS             complement(27375..28244)
                     /locus_tag="VIG70_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8250692.1"
                     /translation="MLHADSARNRLIGIGLVSLCYLLFSLLDGSAKWLVGGMPVIMVV
                     WLRFATHAVFAGIVLFPVKGVSLVKTTHWRWHMLRAAMFIAMTGINFWALQYLQLTVT
                     SSIFFSVPLMIALASAALLGEKLDAGRWIAIAAGFAGVLVIVRPWGAEFHPAMLAAVV
                     NAILYAGFLMMTRRLAAYDSPETIQYLPAVGAAIGLAPFALASWESPSGWLEWTVACL
                     LGVLGGCGHYLLALAHRYAPSSVIAPFLYQQVIYMAAFGYLVFGDVPGPGVWIGAPIV
                     IASGLYLFSRERK"
     gene            complement(28231..28542)
                     /locus_tag="VIG70_08805"
     CDS             complement(28231..28542)
                     /locus_tag="VIG70_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250693.1"
                     /translation="MKNLVAKLEVIGVRPDGERVPLMAEVGRPKEARDGRWACAIAIA
                     PLETQPEDVRGTDSFHAVWLALALVLKLLDNLKDEGWRLENADGSEFPLEDYKAGLAS
                     R"
     gene            complement(28539..29168)
                     /locus_tag="VIG70_08810"
     CDS             complement(28539..29168)
                     /locus_tag="VIG70_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8250694.1"
                     /translation="MPTALITGAGRGLGKELARQYAAEGWKVIGTERRDMDMTNRRQI
                     REFAARLKGEPLDLLFCNAGITGRKGMAPGSFDFESWEEILRVNVLGAAAVAEALLDN
                     VAASGRKTIAMMSSRLGSISESSGMTLPYSTSKAALNMLVKGLAATLASRGIVVVALS
                     PGWVRTDMGGPQAPLTPEKSVQGLRKVIAELRTADSGKFFSHDGSQIPW"
     gene            complement(29170..29790)
                     /locus_tag="VIG70_08815"
     CDS             complement(29170..29790)
                     /locus_tag="VIG70_08815"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY8250695.1"
                     /translation="MQHFRYLPLAGTLILFAIVFVWRPWLQRRRHGTWGVVLFKGSRA
                     QKVRDGMLFVLPVLLVGQAVVAAGAPESLPLSEADLRAAPGVRHALGAVLLFGGLILQ
                     AAAMLDLGASWRIGIEERARPGLITGGLYRFSRNPIFLALIAVLAGYTLLLPTMLSAL
                     ILVGACIAIRQQIAEEESYLLRTYGEQYLQYARRVGRLLPGIGKLD"
     gene            complement(29777..30826)
                     /locus_tag="VIG70_08820"
     CDS             complement(29777..30826)
                     /locus_tag="VIG70_08820"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEY8250696.1"
                     /translation="MNTYRIAAIPGDGIGNEVIPAGMEVLQALSSKEKFRIDAKVFDW
                     SSKRYLETGKYIPPGGLEQLKKFDAIFFGAVGAPDVPDHVSLWELRLAICQGFDQYAN
                     VRPSRLLPGISSPMKNAGEIDWVIVRENTEGEYSGSGGRVHQGLPDEIATETSVFTRK
                     GVERVHRFAFELAHKRPRKHLTLVTKSNAQRHGMVLWDEIFYAVAKEFPEVKTDRILV
                     DAATTRMVLKPAAIDVMVASNLHADILSDLAAALSGSLGIAPTANLNPERKFPSMFEP
                     IHGSAFDITGKGIANPIATFWTASMMLEHLGEAKAAARLMKAIEAVTAKKIFTPDLGG
                     AARTRDVTEAVLAAL"
     gene            30874..32040
                     /locus_tag="VIG70_08825"
     CDS             30874..32040
                     /locus_tag="VIG70_08825"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8250697.1"
                     /translation="MLGGAVLLAACGPSGDSSTGAPPGEPPVLTAQRVFPALSFAQPV
                     ALLQAPADGTRWFVVEQAGRVRVFANISQAAVFDTFVDISARVTSGGEMGLLGMAFHP
                     NFPANPRVYLSYTNETAGIRVSRISEFRLDVSGDLDPGSEIVLLSIGQPETNHNGGQI
                     AFGPDGFLYIGIGDGGGGDDQHGAIGNGQLMTTLLGKMLRISVTPGIYSIPPGNPFAA
                     NPPCGMGGTGTLPCPEIYASGFRNPWRWSFDRRTGALWVGDVGQGALEEIDRVTLGGN
                     YGWRCREGTRPTGLACGSEQGLLPPVAEYGRTEGSSVTGGYVYRGSAIADLPGRYVFG
                     DFVTGSLWHIAGETSPTRVMRAGFPTGLNIASFGEDVAGELFIVDYGGGIYRLR"
     gene            complement(32042..33121)
                     /locus_tag="VIG70_08830"
     CDS             complement(32042..33121)
                     /locus_tag="VIG70_08830"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEY8250698.1"
                     /translation="MKEHSIAVIPGDGIGREVMPEGVRALEAVGARHGLRFKWQEFPW
                     SCDWYKAHGKMCPDDAPDILRRFDAIYFGAAGNSAIVPDHISAWGLLINFRRWFDLYV
                     NLRPVRLFEGLPCPLAGRKPGDIDYVVVRENTEGEYGNVGGRIFEGTASEAVMQQSVF
                     TRKGIDRIMKYSFELARTRKKHLTSCTKSNGITITMPYWDERFAEMKKKYPDVRSDQY
                     HVDGLSIQMVLNPDRFDVIVASNLFGDILSDLGPATAGTIGIAASGNINPERTAPSIF
                     EPVHGSAPDIAGKKIANPIGAIWSGALMLEHLGHAEAAADLVRSIERVIVDGPRTPDM
                     KGKASTSEVGAAVAEAVRSASPVAA"
     gene            33147..33803
                     /gene="tsaB"
                     /locus_tag="VIG70_08835"
     CDS             33147..33803
                     /gene="tsaB"
                     /locus_tag="VIG70_08835"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY8250699.1"
                     /translation="MRFAAIETSTEWCSLAVWRDGEIAALERRAGHRHSELALPMLET
                     LLGKDKLDAVAFGAGPGAFTGLRIACALAQGLAFARGLPVIGISSLEALAQESGAARV
                     VACIDARMREVYYAALEKRAGRWHEVIAAQCVAPQSAPRPPGEGWVGCGNGFEVYGEA
                     LKAKVSVMKPVIHPTAVAVAQLAAPRLAAGEGVDAALAAPVYVRDKVAFTKDELERRS
                     "
     gene            33826..34251
                     /gene="rimI"
                     /locus_tag="VIG70_08840"
     CDS             33826..34251
                     /gene="rimI"
                     /locus_tag="VIG70_08840"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY8250700.1"
                     /translation="MRERDLEEVMAIESAIYSHPWTRGNFGDSLRAGYECRVLRLGGE
                     LLGYFVLMVAAGEAHLLNLSITAVRQRAGHGSGLLREAMALARNLGANSLFLEVRPSN
                     RAAQALYTRFGFRKIAVRRGYYPAHAGREDALVLTLPLA"
     gene            34356..34892
                     /locus_tag="VIG70_08845"
                     /pseudo
     CDS             34356..34892
                     /locus_tag="VIG70_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
     gene            complement(35124..36416)
                     /gene="lplT"
                     /locus_tag="VIG70_08850"
     CDS             complement(35124..36416)
                     /gene="lplT"
                     /locus_tag="VIG70_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEY8250701.1"
                     /translation="MNYGFYIIMAAQFFSSLADNALLVAAIALLIQAEAPSWLTPYLK
                     FFFVISYVALAPFVGAFADRLPKGQVMLIANTIKIVGCVTMLWGVDPLIAYAIVGLGA
                     AAYSPAKYGILTEYLPHRQLVVANGWIEGLTVASIILGTMLGGLLIHVRVSGVLLHFD
                     MPLIETSIDTPAEAAIALIVVVYAIAALFNLEVPKTGVVLKKLPAHPLDTLKDFWRCV
                     GHLWRDRLGQISLAVTTLFWGAGATLQFILIEWARAALGFDLSKAAILQGVTAFGIAL
                     GAVLASLWVALDRSVRVIPLGIAMGLIVLVMIVVKQVWLAVALMVVVGALAGFFVVPM
                     NALLQHRGHHLVGAGRSIAVQNFNENSSILVMIALYSLLLSVGYSIYTVVLIFGLFVA
                     GTMTVVQIWYLRNRRRHREEIDRLLAFARAEDPHKPGK"
     gene            36494..37567
                     /gene="alr"
                     /locus_tag="VIG70_08855"
     CDS             36494..37567
                     /gene="alr"
                     /locus_tag="VIG70_08855"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY8250702.1"
                     /translation="MPRPIRATISAAALAHNLTVARRRAAGAKVWAVVKANAYGHGIE
                     RAARALAAADGFAVLDFQEALRLRAAGVAQPILMLEGFFSAADLELLPKHALTPVIHN
                     PEQVELLRRSPLAGEIDVYLKVNSGMNRLGFGVESLGPAYNSVRMHPQVRNVTFMTHF
                     ADADGAGGVQAQLEWFDELTKSFEVHSRSLANSAALLRFPEARGDWVRPGIMLYGCSP
                     FSGQSAEQLGLKPVMTLSSEIIAVQQLQPGERVGYGFSYEAVGEMTLGIVACGYADGY
                     PRHASTGTPVLVNGKRTRLVGRVSMDMISVDLSGLPGAYIGTPVTLWGEGLSADEVGA
                     AAGTLSYELLCKLSTRVPIVEVT"
     gene            37564..38913
                     /gene="radA"
                     /locus_tag="VIG70_08860"
     CDS             37564..38913
                     /gene="radA"
                     /locus_tag="VIG70_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY8250703.1"
                     /translation="MKKPKTAYVCSECGATALQWFGSCPSCGTAGTLSESIAERGSGF
                     RPKSAPETVGLAEVQLKDLERIPTGIGELDRALGGGLVAGQVALLGGDPGIGKSTLLL
                     QALTSMAGTGALIYVTGEESVEQVALRARRLGLEAANVRIAAETQLERVLGALEAARP
                     RVAVIDSIQTLWSESLQSAPGSVAQVRECAAQLTRHAKRAGTALFLVGHVTKEGAIAG
                     PRVLEHIVDTVLYFEGDAHSSFRLVRAAKNRFGAVNELGVFAMTEKGLRGVSNPSKLF
                     LSDHDKPVAGSCVLATLEGTRPLLVEIQALVDPAHTPNARRLSVGLEANRLAMLLAVL
                     HRHAGIATWEQDVFVNAVGGVRIGEPAADLAVCLAVVSSFSDKPISSRVAVFGEIGLA
                     GEVRPAVRGQERLREAAKLGFERAIVPKANLPKAKIAGLETLAVERIDQAVRLLREL"
     gene            complement(39331..40365)
                     /locus_tag="VIG70_08865"
     CDS             complement(39331..40365)
                     /locus_tag="VIG70_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8250704.1"
                     /translation="MEQRQLGGSGFMVPALTFGTGTFGGANEFFKAWGASDVQEATRL
                     IDVCLESGLNMFDSADGYSNGKAEEILGQAIKGRRDKVLISTKGSFRRGPGPNDVGSS
                     RHYLPRAVDASLRRLGTDYIDLYQLHAFDALTPVEEALGALDDLVRAGKLRYIGCSNF
                     SGWHLMKSLAVSEKYGLARYVAHQAYYSLIGRDYEWELMPLALDQKVGAVVWSPLGWG
                     RLTGKIRRGHPLPKVSRLQSQLAIDKGPPVPDEYLYRVVDALDAVAKETGKTVPQVAL
                     NWVLGRPSVSTVIIGARNEEQLRQNLGAVGWKLTAEQVAKLDATSAQVPAYPYWHQRG
                     TFPERNPPPV"
     gene            complement(40378..41589)
                     /gene="hpnE"
                     /locus_tag="VIG70_08870"
     CDS             complement(40378..41589)
                     /gene="hpnE"
                     /locus_tag="VIG70_08870"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEY8250705.1"
                     /translation="MGDRVAIVGAGYAGMAAAVTLAERGVPITVFESGPVPGGRARRV
                     ISQGHTLDNGQHIFVGAYSQLFGLMRTVGVPPDALLRVPLEIRYANGFAFRAAPFGRL
                     AGLLLARGVPFTERLGTLRFVATLRRRNWRMQPDITVQALLDAHGQRGRMRHYLWAPL
                     CVSALNTPLDQASANVFLAALRDTLAGDRDASDLLLPRVDLSRLFPEPAAEFVRARGG
                     EVRYGTTVRELDSLKASFSNVVVAVGPHQLKTLLPEAANGYTYQPIYTCYLQYPESTR
                     LDFPMLGMGEGLVQWVFDRGTLLGEKGRLACVISAQGDHQQMTHDELAETCHRELRHA
                     LPALPSPLWSQVIAEKRATIACTPGLKRPEIQTAGVFLAGDYTDAEYPPTLEAAVRSG
                     VRAASMILKES"
     gene            complement(41574..42407)
                     /gene="hpnD"
                     /locus_tag="VIG70_08875"
     CDS             complement(41574..42407)
                     /gene="hpnD"
                     /locus_tag="VIG70_08875"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEY8250706.1"
                     /translation="MTPDQYCQQKAAASGSSFYYSFLFLPSERRRAITALYAFCREVD
                     DVVDETGEMQIAAAKLAWWRSEVANLDAGNPQHPVTKALGVYKERFSIDAKRLNDIID
                     GMEMDLTQTRYLDWPGLERYCYRVAGVVGLLAAGIFGYRDPRTLEYARDLGIAFQLTN
                     IIRDVGEDARKNRIYLPAADLKQFGVPAADILQTRETPEFNRLMAFEAARARSHYEKA
                     MQALPAADRKAQRPGLIMAAIYRTLLEEIERDGFRVLTGRTSLTPLRKFWLAWKTWVT
                     A"
     gene            complement(42490..43278)
                     /gene="hpnC"
                     /locus_tag="VIG70_08880"
     CDS             complement(42490..43278)
                     /gene="hpnC"
                     /locus_tag="VIG70_08880"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEY8250707.1"
                     /translation="MAVGHYENFPVASLLLPAPLREPIEIIYRFARSADDFADEGDDP
                     PELRLRKLNDYRRQLETPEDPLLRDVAKIIREHELPVELFADLLDAFSQDVTKKRYAN
                     FADVLDYCRRSANPVGRLLLQLFKRTSDSNCRASDAICSALQLVNFWQDVDVDYLNDG
                     RIYLPQDEMARHGVTERHISQKICDDAWRSLMKFQVERARELMLSGKNLGRALPGRIG
                     LEIRATIQGGLRILEKIERARYDVFRRRPTLRAFDWPLLLIRAV"
BASE COUNT         7190 a        14446 c        15007 g         6760 t
ORIGIN      
        1 cacgagcaac ggctcgcggc gctcgcgcgc gaagcgggat tcacccaggt ctccctctcg
       61 cacgaggtga gcccgctgat gaagctggtg tcccgcggcg acaccacggt ggcggatgcc
      121 tacctctcgc ccttgttaag gcgctatgtc gagcgcatcg cagccgagtt gccggacgtg
      181 cgcctgatgt tcatgcagtc gaacggcggc ctcgccggcg cgcgccagtt ccagggcaag
      241 gacgcgatcc tctcggggcc ggcgggcggg atcgtcggcg cggtgcgcac ctcggccgcc
      301 gccgggttcg accgcatcat cggcttcgac atgggcggca cctccaccga cgtgtcgcac
      361 tatgccggcg agctcgagcg cgagttcgag acccgggtcg cgggcgtgcg catgcgcgcg
      421 ccgatgatga gcatccacac cgtcgcggcg ggcggcggct cgatcctgca cttcgacggc
      481 gcgcgctacc gcgtcggccc ggattccgcc ggcgccaacc ccgggcccgc ctgctaccgg
      541 cgcaacggcc cgctcaccgt gaccgacgcc aacgtcatgc tgggcaaggt ccagccgaag
      601 tactttccac gggttttcgg ctgcgagggc aacgagcagt tggacgccgc gatcgttcaa
      661 gaagagtttc gaaagcttgt ttcgaaaatc aaagacggcc gtagcgcgga gcgggtagcc
      721 gaaggtttcg tgcagatcgc cgtcggcaac atggcaaacg cgatcaagca catctcggtt
      781 cagcgcggtc acgacatcac gcgctatacg ctgtgctgct tcggcggcgc ggcgggtcag
      841 catgcctgcc tggttgccga cgcgctcgcg atgacgcgtg tcttcatcca tccgcatgcc
      901 ggcgtgctct cggcctacgg catgggcctc gccgaccagg tggcgatccg cagccgcgcg
      961 gtcgaggcgc ggctcgaagg ctgcgacctg gaagacgcgg cggaggcgct cgcgatcgag
     1021 gcgcgcaacg agctggtcgc acaaggcctc cctccggaca agatcgcggt ggagcgcaaa
     1081 gcgcacctga agtacgaggg caccgacacc gcgctcgtgg tccgtctcgg ccccgcgccg
     1141 gccatgctgc gcgagttcga ggccgcctac cgcaagcagt tctcctttct gatgcccggc
     1201 cggccgctca tcgcggaggc ggtctcggtc gaggcgatcg cccgcggcga agttttccgg
     1261 gaagaagcgc tgccatcgaa agaaaaggca aagcccgcgg aaattgtccg catgttcacc
     1321 ggcgggcgct ggcatgatgc gccgctctat cggcgcgagg acctcggtcc gggcaatcgg
     1381 gtcgacggcc cggcgatcgt tgccgaggcg aacgccacca ccgtggtcga gccgcagtgg
     1441 caggcgagcg tgacggctct gaaccacatg atcctcgagc gcaccacgga aaagaagaga
     1501 agctccgcga tcggcacttc ggtcgatccg gtcatgctcg agatcttcaa caacctctac
     1561 atgtcgatcg ccgagcagat gggcctgcgc ctgcagaata cggcgtactc cgtgaacatc
     1621 aaggagcgcc tcgatttctc ctgcgccctg ttcgacgcag acggcaacct gatcgccaac
     1681 gcgccgcaca tgccggtgca cctcggctcg atgggtgaat cgatcaagac cgtgatccgg
     1741 ctgaacgccg ggaagatgaa gccaggggac gtcttcgtgc tcaatgcgcc gtataacggc
     1801 ggcacgcatc tcccggatgt caccgtgatt accccggttt ttgattcaaa cggaaaagaa
     1861 atcctctttt acgtcggctc gcgcggccac cacgccgaca tcggtggcat cacgccgggc
     1921 tcgatgccgc cggggtcgaa ggtggtggaa gaggagggcg tgctgatcga caacttcctc
     1981 ctggtcgagc aggggcgctt tcgcgagaag gagacggtcg cgctcctcac ttccggcaag
     2041 cacccggtgc gcaacgtgca gcagaacatc gccgacctgc gagcgcagat cgcggccaac
     2101 gagaagggcg tgcaggagct gcgccggatg gtcgagcagt tcggcctcga cgtggtgcgc
     2161 gcctacatgc gccacgttca ggacaacgcc gaagaatcgg tgcgccgcgt gattggcgtc
     2221 ctcaaggacg gggatttcga ttacccgctg gataacggag cccggatcaa agtccgtatt
     2281 caaataggca aagacaagag aagcgcaaag atcgacttca ccggcacctc ggcgcagctg
     2341 ccgaataact tcaatgcacc cgcggcggtc tgcatggccg cggtgcttta cgtgttccgc
     2401 acgctggtgg acgacgagat cccgctcaac gccgggtgcc tcaagccgct cgaggtggtg
     2461 attccggagg gctgcatgct gcggccgcgc tatcccgcgg cggtggtcgc tggcaacgtc
     2521 gagacttcgc agtgcatcac cgacgcgctc tacggcgcgc tcggcgtgct agcggcgtcg
     2581 cagggcacca tgaacaactt caccttcggc aacgagcgct atcagtacta tgagaccgtc
     2641 gccgggggct ccggcgccgg cccggacttc gacggcgccg acgtggtgca gacgcacatg
     2701 acgaactcgc ggctgaccga tcccgaggtg ctcgagtggc gcttcccggt ccggctcgag
     2761 agcttcggga tccggcacgg ctcgggcggc ggcggccgcc ggcgcggcgg gcacggcgcg
     2821 gtgcgccgcg tgcgtttcct ggagccgatg acggcggcga ttctgtcggg gcaccgcctg
     2881 gtgcagccgc acggcctggc agggggagaa gccggcgccg cggggcgcaa ctacgtgctg
     2941 cgggcaggcg gcggggtgag cgagctcggg cccttcgagc agaccgagat ggccgcgggc
     3001 gacgtattcg tgatcgagac tcccggcggc ggcggctatg gcgctgtaaa atcggggaaa
     3061 gaatgaaagc gagcggcaca tgaacgcgag cgcgatctac tcgaagtcgg ggaagggagt
     3121 ccaggaagct tcgggcaaga ccaacgtcct ccagcgctcg gaccgggcgg tgctggccgc
     3181 gatcgacggc cgcgcgacgc tcgccgacgt ggcgcagaaa gtcggcaagc ctttcgatcc
     3241 cgccttcgaa aagctgatcg agtcgctcga caaaggcggt ttcatccgcg aggtatcggc
     3301 tggagcgccc ggcgtggccg gcgtaacgac caaagcgccc gccgcgggcg gcaagcccgc
     3361 caccaagagc gcgccggtgg acccctcgtc ggatctcgac ttcaccaccg cgctgtcgat
     3421 cccgaagggg aaacctgcgc cgccgccgcc gaaagccgcg ccgccgccct cggccgccga
     3481 gaaggcgcag gccgcgaagt cggccgtgga gcaagaggac gccttcgcca aggcgcgcga
     3541 ggaagccgag cgcaagaagc aggcagaccg cgaccgcctg aaggcggagg ccgaagccaa
     3601 gatgcgcgag gagaccgagg ccaagctgcg agccgagacc gcagcgaggc tcaaggccga
     3661 agccgaggcc aaggccaagg cggatgccga agccaagatc aaggccgcgc gggacgccgc
     3721 cgccaaggcg gccgcagaag ccaaggcgaa ggccgaggcc gaggcgaaga gagcgcgcga
     3781 ggaagccgag cgggcccgca aggaagccgc ggaggtagcc gagcgcgccc gcaaggaagc
     3841 tgcggaggta gccgagcgcg cccgcaagga ggccgaagcg aaagccgagc gtgcgcgcaa
     3901 ggaagccgag gagaaggcgc gccacgagca ggaagagctc aagcgcaagc tcgaggagga
     3961 gcgcacggcg cgcgaagagg ccgagcgccg ggcgcaggag gaggccaagc gcctcgaaga
     4021 ggagcgcagg aagctcgagg aagagcgcaa gcgccacgcg gaggagcggc gccgcgagga
     4081 ggaagagcgc acgttgcggc gcatccagga agaacgcgag cgcgtcgacc gcaagcgcga
     4141 cgaggcagcg cgcaagaagc gccgggagga ggaggaagcc caggaagagg agaagcccag
     4201 gacaaagcag aaagagccgg agccggatcc ggagccgacg gcgccgccac cggtggcggc
     4261 cgcgggcggg gcattctccg acacgctgct cgccgatctc gactcgttca ccacgcgcga
     4321 cgaccaggac cagaaggaca aggaagaggc ggcccgcaag gagcaggaag cggccgcggt
     4381 gcgggcgcgc gaggcggccg cgctgcgcgc gaaggaggac gccgagcggc aggaccgcga
     4441 ggcgaagcag cggcgcaagc aggaggaggc cgagcgcaag gcgctcgagc aggccgagct
     4501 gcgcgcggcc gaggagcgcg agaaggagcg cctgcgcgag gaggaagaga agcgcaagca
     4561 ggaggccgag gagcgcgagc gcaaggcgaa gcaggaggag caggtcacgg cgcagtcgac
     4621 gcggagcggc gcgatgttcg acgccgagat gcgcgccaag cgcgagcgcg agctcatggc
     4681 gcgcgcgccg agggctgccg cagggacgag ggcggagaag aagggctggg gcaagccgat
     4741 cgcgctcacg ctgttcctgc tgctggtcgt cgcgctcggc gtggcgcacg tgctgccgat
     4801 cgacaccgca gagtacgagc gcgcggcgag cgatgcgctc ggccggccgg tcaagatcgg
     4861 gtccgcgagg ctctcgctca tcaccggtct gcagatccgg ttcagcgagg tcaacctcgg
     4921 cgagggcgtc agcatgtccg aggtgcgcgc atttccggag ttcgacgccc tcacggggcc
     4981 ccgcaaggcg ttcagccgga tcgaggccga ccgcatgaag atcgagcagg acatggtggg
     5041 acaggcgctg ttcgccaagg tcaaggcgga caacttcagc gtcggccagg tggtgatgaa
     5101 gaacgtcgag ttcaccgggc cgctcgtcct gcccaagggg ctcgagctcg agctggtcta
     5161 cgacggcgcg ggcatggtgc gctccgctgc ggtgcgcggc cccgaggccc tctccgccaa
     5221 gctgacgccg aagggctcga gcaccgaggt ggagatgacg gcggcgagct tcacgctgcc
     5281 gttcgcgccc gagatcgcgc tcagcaagtt ctcgatgaaa ggcacggcga cgccgcaggg
     5341 gctgtcggtg acggaatggg acggcgcgct cctgaacggc aacgtatcgg gcacggcgag
     5401 cgtgcgctgg ggagaaagtt ggaccgtcga gggtgtggtg accgcgcgca acatcaacgc
     5461 cgcggtgttc gcgccggcgc tcctgtccga cggcaaggcc gaaggcagcg gcaagttctc
     5521 gatgcgcggc ccgccgggca agctcaccgc gaccagccgc ctcgacggca gctttaccgt
     5581 gacgcggggc gtgctcggta gcatcgacct gtcgcgcgcg atccagactg gcggcaaggt
     5641 ggcgaccggc cgcacccagt tcaacgagat gaacggccag gtgacctatg accgcggcgc
     5701 ggtgtcgctg cgcaacatca ccatcggcgc cggccagctc aacgccgggg cgagcgctga
     5761 tatcgcgcag aacggcgcgc tctcgggccg catcgtcgcc gacgtcaggg ttgcgcagca
     5821 gcgcttgagc gcgacgctga atcttggcgg cacactgtcg gagccccagg tcaagaatta
     5881 gcaggaaccg ctacgtgcaa agcatcacgc agctccgagg cccgcgcgcg ctctcggagt
     5941 ctcgcctcgc caaactgctc gcgtccctgc agaaactcga tcccggcgtt cgctcggctt
     6001 cgaccgagta ccgctatttc gtcgagaccg ccggcgaact cggcgtgccc gggcagcgct
     6061 tgctcgagcg gctgctcgac gacggatcgc cccggccgca gaccgcttcc ggcgccctct
     6121 atctggtggt gccgcgcctc ggcaccctct cgccctggtc gtcgaaggcg agcgacatcg
     6181 cccgcaactg cggcctggag caggtgaggc ggatcgagcg cgggactgcc ttccatatag
     6241 agggcgccaa aaccgacccc tcgcctctgt tacacgaccg catgacgcag accgtgctgc
     6301 gttcgttcga ccaagcggcg aaactcttcg agcacgtgcc gccgaggcct ctgcagcatg
     6361 ttcccgatat ccgcagggcg aatcgcgacc tcggcctcgc gctgagcgag gacgagatcg
     6421 aatacctcga gcgcgcctat cgccgcctcg ggcgcgaccc gaccgacgtc gagctcacca
     6481 tgttcgccca ggcgaactcg gagcactgcc ggcacaagat cttcaacgcc gactggatca
     6541 tcgacggcgc gcgccagccg cagtcgctgt tcgccatgat ccgccacacc cacgccgcga
     6601 gcccgcaggg cacggtgctc gcctattccg acaacgcggc gatcctcgag ggccgccccg
     6661 cgagccgctt cttccccgac gaaaagcagg tctacggcag gaacagcgag ctcacccaca
     6721 tcgtcatcaa gtgcgagacg cacaaccatc cgacggcgat ctcgccgttc ccgggcgcgg
     6781 ccaccggcgc gggcggcgag atccgcgacg aaggctcgac cggccgcggt gccaagccga
     6841 aggcgggcct ggtcggattc tcggtgtcgc acctgcgcat tcccgggcac gagcgcccgt
     6901 gggagcgcga cggcgtcggc aagcccgatc gaatcgcctc ggcgctcgac atcatgctcg
     6961 acgggccgat cggcgccgcg gcgttcaaca acgagttcgg ccggccgaac ctgtgcggct
     7021 atttcaggac attcgagttt cagaaccggg gctaccacaa gccgatcatg ctcgccgggg
     7081 gcgtgggcaa catcgccgcc gggcacgcgc acaagcagcc cgttcctccg gggagcctct
     7141 tcatccagtt gggcggtccg ggcctcgcca tcggcatggg cggcggcgcc gcctcgtcga
     7201 tgggcgccgg agcgaacgtc gaggacctcg acttcgactc ggtgcagcgc gacaacgccg
     7261 agatgcagcg tcgcgcgcag gaggtgatcg accgctgctg gcagctcggc gccgccaacc
     7321 cggtcctgtc gatccacgac gtcggcgccg gcgggctgtc gaacgcactg cccgagctcg
     7381 cgcacggcgc cgggcgcggc gcgcgcctcg agctcagggc gatcccgtcg gaagacagcg
     7441 gcatgtcgcc gcgcgagatc tggtgcaacg aggcccagga aagatacgtc ttggcgattt
     7501 cgaagcagga cttggaggcc ttcaaaaaaa tctgcgagcg cgagcgttgc cctttcgccg
     7561 tggttggcac cgcgaccgac gacggccgct tggtggtcga agattcgagg ctcaagcaaa
     7621 cgcccgtcga cgtcgagctc gaaatgatcc tcggcaagcc gccgaagatg ctgcgcgacg
     7681 tgaagcgcgc gcaccccagg cccgccgcga tttcgctgaa cgagatcacc ctggaagctg
     7741 cggtgagccg cgtgctgcaa caccccgcgg tagccgacaa gaccttcctc gtgtcgatcg
     7801 gtgaccgcac ggtcggcggc ctttgcgcgc gcgatccctt cgtcggcccc tggcaagtgc
     7861 cggtggccga ctgtgcggtg acgcttctcg actacgaagg ctatgccggc gaggcgttcg
     7921 ccgtcggcga gcgcacgccg ctcgcgctga tcgacgcccc ggcttcgggc cgaatggcgg
     7981 tggctgaggc attgaccaac ctcgctgcct gtggggcatc tctatccacg acaaaattat
     8041 ccgcgaactg gatggccgct gccggttacc ccggcgagga cgcggcgctc tacgacaccg
     8101 tcagagcggt ggcgctcgag ctttgcccgg cgctcggaat ctcgatcccg gtgggcaagg
     8161 attcgctgtc gatgcgcacc gcgtggggcg acaaggaagt ggtggcgccg atgtcgctcg
     8221 tggtgtcggc ctttgccccg atcgcggacg cgcgcgcggc gctcacgccc cagttgcgca
     8281 ccgacgcggg cgataccgag ctcgtgctcg tcgacctggg gcgggggaag aaccgcctcg
     8341 gcggctcgat cctcgcgcag gtgttctcgc agctcggcga cgactgcccg gacctcgacg
     8401 atcccgcgct cctgaagggc ttcttttcct tcgtgagcga aaaccgcgag cagctgctcg
     8461 cttatcacga ccgctcggac gggggtctct tcgttacggc gtgcgagatg gccttcgccg
     8521 gccgctgcgg cgtcacgctc agcctcgatg cgatcgcctt cgccgccgcc gccgacgacg
     8581 tcgacgcctt caagcgcgac gccggcgagc agcttgccgg ccgcgcgaag gacctcgcgc
     8641 tgcaggcgct tttctccgag gagctgggcg cggtgctgca gatccgcgct tcggaccgcg
     8701 cgcgcgtcat ggccgcgctg cgcgcggccg ggctgggcga gcattcgcac gtcatcggca
     8761 gcctcaaccc gcgcgacgag ctccggatca cgcgcaacaa ccgcgtggtg ttcgccgcca
     8821 agcgcgccga gctgcggcgc acctggagcg agaccacctg gcgcatgcag acgctgcgcg
     8881 acaacccgga gtgcgcgcgc gaggagtacg accgcatcct cgagacggac gatccgggtc
     8941 tttccgtgct tttggctcat gaagcaaaaa atattgcgat ctcgaccgga acccggccgc
     9001 ggatcgccat cctgcgcgag cagggggtga acggtcaggt cgagatggca gccgcgttcg
     9061 accgcgcggg attcgagtcg gtcgacgttc acatgaccga cctgatcgcc gggcgcgcgt
     9121 cgctcgaggg attcaagggc ttcacggccg gcggcggctt ctcctacggc gacgtgctcg
     9181 gcggtgggca gggctgggcg aagacgatcc tcttcaacgc gcgggcgcgc gaggagttcg
     9241 agcgcttctt cgcgcgccag gacactttcg cgctcggcgc ctgcaacggc tgccagatga
     9301 tgggtgcgct gcgcgagctc gtcccgggcg cctcgggctg gccgctcttc gtgcgcaacc
     9361 gctccgagca gttcgaggct cgcctcgtca tggtcgagat cgtagagagc ccctcgatcg
     9421 tgttcgcggg catggccggt agccgcattc ccatcgtcac cgcgcatggc gaaggccggc
     9481 cgaacacgga aaacccctca atcgtttgca tgagatacgt gaataaccgc ggcgagccca
     9541 cgcagaccta tccctataac ccaagcggct caccgggcgg catcaccggc ctcaccacgc
     9601 cggacgggcg cttcaccatc gtcatgccgc accccgagcg cgcgttccgc aacgtgcaga
     9661 tgtcgtggtc gccgccgggc tcgggcgaag actcgccgtg gatgcagctt ttccgcaacg
     9721 cgcggaaatg ggtcggctag ggttgcatgc agcgcagtga cgtcctgatc gtcggcgccg
     9781 gaatcggcgg cctgacgcta gcgctggagc tcgcgcgccg caggatcccg tgccgcgttt
     9841 tcgagtcggc ccctgagatc aaggcggtcg gcgtcggcat caacctgctg ccgcacgcta
     9901 cccaggtcct cggcgagctc ggcctcgagc cggcgcttgc gcgcgtcgcg gtcgagaccg
     9961 ccgagagcgt gttcttcaac cgcttcggac agctcatata taaggagccg ctcggccgca
    10021 aggcgggcta cccgtggccg cagttctcga tccatcgcgg cgacctgcag gcgctcctgc
    10081 tcgacgcggt gaaggcgaag atcggcgcgg agcgcattca cctcggctgg cactgcctcg
    10141 ggttcgagca ggatgcttcc aaagttacgt tgaggtttcg cgatcgtgcc cccatggacg
    10201 gaacaatcgc ggtaggctgc gacggaatcc actcggtgat ccgcaagcag ctctatcccg
    10261 cggaaggcga gccgagatac agtggggtga acatgtggcg cggagtgacg cgctggaagc
    10321 cgatcctctc cggcgcgagc atgctgcgcg cgggctggct cgccaacggg aagctcgtgc
    10381 actacccgat ccgcaacgac gtggacgaaa ccgccgacgg cacggggcgg cagctcgtca
    10441 actggctgtg ggagatcgag accccgcagt acaagcgctg ggactggaac cggccggcgc
    10501 gggtcgagga cttcatcgcc ggtgcagaga gctggcgctt cgactggctc gacgtaccgg
    10561 ctttcctgcg cgcttcggag ctggtgctcg agtacccgat ggtcgacaag gatccgctgc
    10621 ccgcctggag cttcggccgg gtgacgctgc tcggcgatgc ggcgcacccg atggtgccgc
    10681 gcggctccaa cggcgccggc caggcgatcc tggatgcgcg cgcattggca caatgcgttt
    10741 ccgaagagaa gaattccttg aaaggctatg aaagcgagcg gctcgggccg acgtcgaacg
    10801 tggtgctcga gaaccgccgc aacccgccag acgccatcct gcgcgaggtc tacgagcgca
    10861 ccggcgatcg gcccttcgag cgaatcgacg cggtgatcag cgcgcaggag cttgccgcgc
    10921 tttcggaccg ctacaagcag gtcgccggct acgacaagga aaggctgcag cgatgagctt
    10981 cctccaccgc ctgttcccgg ccggccactt cgtcgtgagc gttctgtttc tcgtcgccgg
    11041 cgctgcgctg atcgtgatcg ccggcatgca gctctggcag ggcatccagc ccttcgaagc
    11101 cgcggcgatc agccagcgcc tcaatgcggt gctggaaagc atcgccgtgc taaccgtggc
    11161 ggtggcggcg ctcgagcttg gccagaccat cctggaggaa gaggtgcatc gcggggcgca
    11221 gatgagcgcg ccgacacgcg tacgccgctt cctgtcgcgc ttcatggtgg tgctggtggt
    11281 cgcgctgtcg ctcgagacca tggtgctggt gttccgcgtg agccaggagg cgcccgagca
    11341 gctaccctac gtcgcctccg tcggcgccgc cgccgcgctg ctgctcatcg cgtggggcgt
    11401 attcatccgc ctgaaccgca gcgccgagga gctcgagccg gaggcgatgc aggccgccaa
    11461 gcgcgaagac cgcaagatcg agaaatccgc caaaggagaa gccaaatcat gagcatcacg
    11521 cgccacgatc cgaccagcat cctttctaat gcggtgcagc acggcgacac cgtctacctc
    11581 gccggcgtgg tcgcgaagaa cctcgaccag gacgtcaagg ggcagacgaa gcagatcgtg
    11641 gacgagatcg accgcctgct cgccaaatgc ggctcgagca agtcgaaagt cctctcggcc
    11701 acgatctggg tcaccgacat ccgcaaccgt gcgccgatga acgaggtctg gacggcctgg
    11761 gtagacgcca agaacctgcc ggcgcgcgcc tgcgtcgaag cgaagcttgc agatccgaaa
    11821 gcattggtcg agatcatggt gatcgccgcc cgatagcgct gcataatgcc tgtcagtggc
    11881 cggcggagtc ggcctacagt gggctgtgcc tcttggggag ggggggctcg tgctcatcaa
    11941 cattggcgac atcgcggcag agtgcccgca gtgcggcggc gcggacttcg agacgctttc
    12001 cggcgggccg ctgcgcctcg cgaccgagct gcgctgcacg ggctgcggcg agcacgtcaa
    12061 atacctgctg ctgctcgacc acatcggcga agaggcgatg cgccgcgcca acaggtcgct
    12121 cgacgagctc aagaagcaac cgaagaagta ggtttttgac aaaggctggc gagtttcttt
    12181 caattcctga agttctcctg acagcggctc gcgcgggcgt cgcccatgat gcagcgatgc
    12241 gtcgagtcgc cgtgctggtg atcgagaagg gtgcggaagc aggcctgatc tgcgccgcgc
    12301 tggcgcgcag cccggacgtg cgcgtgatcg atgcgccgga cgtagccgac gcgctggagc
    12361 gcctggatcg ggacccggcc ccggtggccc tcgccatcgc gggagaaatg acgctgagcg
    12421 cagacgcggg tgaactggtc aggcgcctgg gcggacgagg tattcccgtg gtcggggttg
    12481 cgcccgggct gcccgcagcg gcccggcagc gcgcactggc tgccggcgtg cgcgagatcc
    12541 acgaccggcc ggctgagtgg cggccgtact cggccctcat cgactcgctc gtcgcccgct
    12601 tcactccggc gggtctgcca ccccgctagg tcaggactcg gatgaatacg cttgtccttc
    12661 cacgcagctg gctgatcctc gcttcgatcg tcgtcttttt cgccatcgcg acgcgctcca
    12721 gcgacccgcg tgtcggcgac tcccttgtga ctgcgcagag aatcaacgac gtgtcgatcg
    12781 cggaagcgat cgctctcatc gatgccggcg cgctcgtgat cgacgtgcgg gaaggtccga
    12841 agacccacct gcctggcgca atgctgatcc cgctggaggt cctggcggcg catctttcca
    12901 agctcgaggg cgccaaggcg caatcgatcg tggtttattg cggcaacggc acctcgcgcg
    12961 ggccaagggc agcgcacatc ctgacgcagg ccgggtttac ccacgtggtg aacctggagc
    13021 cgggaatcga aggctggcgc gcggcgaagc tgcccgtcgt agcgagctga gctactcgaa
    13081 caggttcgcg acgccgtcca ggctggtcca gttcaacgcg cagctcgcct gggcccgcac
    13141 cacgggcttg gcgtgaaacg cgaccgagag cccggcttcg ttcatcatct tcaggtcgtt
    13201 cgcgccgtcg ccgatggcga cggtgtgctc tttccgcgca cccagccccg tggcgacctg
    13261 cagaaatttc ttcgccttcg catcggcgtc gacaatctcg ccgatcagtg cgccggtgag
    13321 cttgccgccc ttgacctcga gcacgttgga gatcgtgtag tcgatcccga ggcgtgactt
    13381 gaggcgctcg gtgaagaagg tgaatcctcc cgagaccagc agcagcttca cgccgtgttt
    13441 cctgcaggcg gcaatcagcg tgtcggcgcc cgcggtgagc ttcagccgct cctcgtagac
    13501 ctcattcagg gaagtctccg gcagtccagc aagcgcggcc acgcgccggc gcaggctttc
    13561 gcggtactcg atctcgccgc gcatcgcctg cgcggtgatc gccgcgatct cggccttctt
    13621 tccagcgaag tcgccgagct cgtcgatgca ttcgatggtg atgagcgtcg aatccatgtc
    13681 catcgcgagc agcttcaggt cggcgaagcg gcggccttcg gggacccagg cgtggtcgat
    13741 atggtgcgtc tcgcaatagg aagggatcgc cgcgtccttg cgggcacttc tcaagcgaaa
    13801 ggcgcgcgct gtgatgccgt cgactccgga ggggctggcc atggcggcga gcgcatcgac
    13861 gtctgccttt ccgagagcag ggccctgaac caccaagttc atgcggcgag ctggctgagg
    13921 atgtccttta cggcctgggc gcgctcctgc caggcgggga gcttcgcctc gacgcgcagc
    13981 ttctccgggc cggcgagcct cgcgctgcgg cccttcttgc ggatgaattc gatcaccttg
    14041 gcgccgtcga gcggcgggtt cttgatgaac tggagctgca ccgcttcgtg cgtggcgtcg
    14101 acccgcgcga tgcccagcgg ccgcgcggcg atgcgcacgc ggtggcattc gatgagcgcg
    14161 cgcgcggggt cgggcagctc gccgaagcgg tcgaccagct cctcgcgcag agtatcgagc
    14221 tgctcgcgcg tgtcagcgtc cgccaggcgc ttgtagagcg aaagccgctc gtgcacgtcg
    14281 ctgcagtaag tctcgggcag cagcgcgggg acgtgcaggt tgacctcggt ggagacgtcg
    14341 acggcgtcgt cggcctcggg cgccttgccc gccttcagac gcctgaccgc ccgctcgagc
    14401 atgcgcgcgt agagcgagaa gccgacctcc tggatctcgc ccgattgctg ctcgcccagc
    14461 acctcgcccg cgccgcggat ctcgaggtcg tgcatcgcga ggtaaaagcc ggagccgagc
    14521 tcctccatca tctggatcgc ctcgaggcgc ttgaccgcgt tcttgttgag cgcttcttcc
    14581 ggcggcgtca gcaggtaggc gtaggcctgg tggtgcgagc ggccgacgcg cccgcgcagc
    14641 tggtggagct gggcgaggcc gaagcggtcg gcgcggttga tcaccatcgt gttcgcggtg
    14701 ggaatgtcga tgccgctctc gatgatggtg gagcagagga gcacgttgaa gcgctgcgcg
    14761 gtgaagtcgc gcatcacgcg ctcgaggtcg cgctcgcgca tctggccgtg cgccaccgcg
    14821 atgcgcgctt ccggcatcag cttcgccagg cgctcccgca tgcgctcgat ggtgtcgacc
    14881 tcgttgtgca ggaagtagac ctggccgccg cgcctcagct cgcgcatcac tgcctcgcgg
    14941 atcaggcctt ccgaccacgg cgcgacgaag gtcttgatcg cgaggcgctt ctccggcgcg
    15001 gtggcgatta ccgagaagtc gcgaatcccc tccagcgaca tcgccagggt gcgcgggatc
    15061 ggcgtcgcgg tgagcgtgag gacgtcgacc tcggcgcgca gtttcttgag cgcttccttg
    15121 tggcgcacgc cgaagcggtg ctcctcgtcg atgatgacca gcccaaggcg cttgagcttc
    15181 acgtccctgg agagcagctt gtgcgtgccg atgacgacgt cgatctcgcc gttctcgagc
    15241 ttcttgatga tttcccccga ctctttcgta cttctgaatc ttgatagttc tgcgattctg
    15301 accggccagt cggcgaagcg gtcggagaag gtctggaaat gctgctcggc gaggagcgtg
    15361 gtggggcaga ggatcacgac ctgcttgcta tccgcgaccg cgacgtacgt ggcgcgcagc
    15421 gcaacctcgg tcttgccgaa gccgacgtcg ccgcagacca ggcgatccat cggcttgccc
    15481 gcggccatgt cggcgatcac tgactcgatc gctgaggcct ggtcgggcgt ctcctcgaag
    15541 ccgaagccgt ccgcgaatgc ctcgaggtcg tgctggcgga tgccgaaggc gtggccgtgc
    15601 cgggcggcgc gcttggcgta cagcgcgagc agctccgcgg cggtgtcgcg cacctgcttc
    15661 gccgcgcgtg ccttcgcctt gtcccactgg ccgctaccga gcttgtgcaa gggcgcttcc
    15721 tcgggctggg cgccgctata gcggccgatc acgccgagct gcgagaccgg gacgtagagc
    15781 ttgtcgtcgc cttcatactg gagcagcagg aactccgtct tgccgtcgcc cacgtcgagc
    15841 gtgaccagac cctggtacct cccgatgccg tgctgctcgt gcaccaccgg ctcgccgact
    15901 ttgagctcgg agaggtcgcg cagcatgccc tcgacggagc tgcgcttctc ggcggcgcgc
    15961 ccgcgccgcc gcaccacgcc cgcatagagc tcggcctccg tgacgaccgc ccagccctca
    16021 gccggcagga ggaagccatg cgacagcggc gacacgccga gcatgcattt cgaggatttc
    16081 tggacgaaat cctcgaagct cgtgcaaagg tcgggcttca ggccgtactc ggcgaaatag
    16141 ctcgccatcg tttcgcgccg accgggcgat tcggcggcga tcatcacgcg caggccggag
    16201 gtttccagga aatgcttcag ccccgcgagc ggatcctgcg cgcggcgatc gactgcgagc
    16261 gcgggaagcg gcacggcgct gacggggccc tggtcttcgt ccggcttgtc gagcacgtcg
    16321 acacgagcga acgcttgcgc gcggacaaag aactcctcgg cgggaaggaa gagctgggca
    16381 ggggcgagca gcggccggtc ggggtcgccg ccggccatct tgtagcgcga gttggcgtcg
    16441 cgccagaact cctggatcgc gccgctgacg tcgtgatgca gcgcgagagt gctgccggcg
    16501 gggaaatagt cgaagacggt agcgacgctc tcgaagaaaa gcggcaggta atactcgatg
    16561 cctgctgccg gcacgccgtt cgagacgtcg cggtagaggc gcttcttcga cggatcgccc
    16621 tcgaacagct cgcgccagcg gctgcggaac ctcgcccggc cggcgtcgtc gagcgggaac
    16681 tcgcgcgccg gcagcatgcg gatttccttc accccgtaga gcgtgcgctg ggtgtcgacg
    16741 tcgaaggtct tgatcgcctc gatcacgtcg tcgtcgaggt cgagccggta gggcagcgcc
    16801 gatcccatcg ggaagagatc gatcagcccg ccgcgaaaac acacctcgcc cggcgagacc
    16861 acctgcgtga cgtgctgata gccggccacc gcgagctgct gcctcaacgc ctcgatcgac
    16921 aggcggtccg cggttttcag aaaaaacgta tgcgccgcca ggtacgaagg cgggcacagg
    16981 cgcacgagcg ccgtggcggc gggcacgatc gcgacgtcga agtcgccacg ctggatgcga
    17041 tagagcgtcg acaggcgctc ggacaccagg tcctggtgcg gcgaaaactg gtcgtagggg
    17101 agcgtttccc agtccggcaa caggcacacg cgcaactgcg gcgagaacca cgcgatctcc
    17161 tcgagcaggc gctgcgcgtc gagcgcggtg gcggtaatga gggcgagcgg tgcggaccgc
    17221 ggcatttcct cctgcgcgag gcggccgagc gcgagcgcgt ccgccgagcc ggggagccgg
    17281 gtatagcgtc tgcgtgcggg aagcgaatcg cggtgcatca gaggcgtgac ctgtgcaatt
    17341 atatcgccct gaattaaaag aacttctgtg tccgcgcctc tgtgtccatc tacgcactga
    17401 ttccagccgc cggcagcggt actcgattgc ctgccgaggg gccaaaacaa tacctgtcgc
    17461 tcgcgggtcg cccgatgctg tggcacgcgg tgcgggccgt ttgcgtgccg ccggtggaat
    17521 cggtgttcgt ggtgcttgcg ccggacgaca aggccttcgc gtcgcacgac tggagcgcgt
    17581 tcgcggacaa gctggagccg ctctactgcg gcggcgaaac gcgccgcgat tcggtgtaca
    17641 acggcctggt cgccgcgatg gcggcgctgg atgccgacga ctgggttctg gtgcacgatg
    17701 ccgcgcggcc ctgcctgccg cgcgaggacc tggaaagcct ggtgcgggaa acgcagaacg
    17761 acgaagtggg agggatcctg gcattgccgg tcgccgagac ggtgaagaaa gtcgccaagg
    17821 acgaaggcgg tgcgcagcga atttccggca ccgaagaccg gtctcagctt tggctcgcgc
    17881 aaacgccgca gatgttccgc gccggcttgc tgatgcgcgc gctgcagcgg gccaaaggca
    17941 cgccgaccga cgaagccgcg gcggtggagc agctcggact gaggccgcgg ctggtcgccg
    18001 gcagccgcga gaacctgaag gtcacctggc acggggatct cgcgatcgcg gagagcattc
    18061 tgaaagcgcg ggcatgagaa tcgggcaggg attcgacgtg catcccctgg tgagcggccg
    18121 caagttgatc gtcggcgggg tcgagatcgc gcaccccaag ggactcgaag gccattcgga
    18181 cgccgacgtg ctgctgcacg ccatctgcga tgcattgctc ggcgccgccg gcctcggcga
    18241 catcgggcgg cactatcccg acaccgatcc cgcgtatgcc ggcgccgaca gccgcgcgct
    18301 gctgcgcgac gtggcgcaga agctgcggga gaagaagctc aaggtggtca acctcgacgc
    18361 caccatcctg gcgcaggcgc cgcgcatggc gccgcacatg ccgcgcatga tcgcgaacat
    18421 cgccgccgac ctcggcatcg cgccggcggc ggtcaacctc aaggcgacca ccacggaggg
    18481 gctcggcttt gtcggccggc tggaaggcat cgccgcccag gcggtcgcgc tagtggactg
    18541 acatgtattc gccttcttac aaccgattgg aggatcgggc ggagctgctg gcgtttctgc
    18601 aggccaacag cttcgcgctg ctggtcaccg ggactggcgg cacgcttcat gcctctcacc
    18661 tcccggtcat ggtcaaagag cacgaaggca agatctctct cgacatgcac atggcgcgga
    18721 acaacccgca gtggcaggag ttcttcgacg acgaggtgat ggtggtgttc tcgggcccgc
    18781 atgcctacgt gtcgccgcgc tggtacgagg agcaggaacg cgtgccgacc tggaactacg
    18841 ccgcggtcca cgcgtacgga gttcccaggc tgatcgacga gaagaagctg aagcacgaaa
    18901 accagcgccg cctggtcgcg atgctcgatc cgcaatggct gccgcgcttc gacgccctgc
    18961 gtcccgagta cgtggagcac atgctcgccg gcatcgtgaa cttcgagatc gccgtgacgc
    19021 gcctcgacac gcgctggaag ctctcccaga accgcagccg gcgcgagcag gagctgatcg
    19081 cggccgagct ggagaagtcg cccgacccgg ccgagcgtgc attggccgag ctgacgcgca
    19141 agcacctgac cggaaattga aagtcgtcgt catgggcgcc ggcctggccg gcgtcaccac
    19201 cgcctgggcc ctcgcccggg acggccacga ggtcgtggtc atcgaccgcg agccggaagt
    19261 cgccgccggg gcgagctatg cgaacacggg catcgtcgcc gcgagccgcg cctacccgtg
    19321 gtcgtcgggc gagatcggcc gcgcgctgcg ccgctcggcg ctgcgcctgg acccggagct
    19381 tctggtgtgg ggcatccagc atctcctcct gaggagcggc gggtcgtacc ggcgggtgct
    19441 cgaggcgaag gtgcgcctcg tgcgctactc gcagcaggtg atgcacgaaa tggtccgcga
    19501 aatcgcattc gacggactca agcgcggcgt gttgtatctc taccgcgaag cgcaggcgct
    19561 cgacgaggcg tgggtgcgcg cgaggtcgat gcgcgatctc gggttcgcgc tcgagcgttt
    19621 cgagccggcc caggttttcc atcacgagcc ggccatcgcc gcgtcccggc tcgccggggc
    19681 gctgtttgcg ccgggcgacg agtgggccga cgcggcgctg ttctgccgct cgctcgccgc
    19741 gaaatgccgg ccgctcggcg tcaacttctc cctggagaac gagatcctct cgatcgagcc
    19801 ggccgacgcg cgcgtgcgcg aggtggtgac gcgccgcggg cgcgtgcgcg gcgacgcctt
    19861 cgtgtgcgcg ctcggggtca tggaccgcaa gctgcgcgag cagctcggca cccacgttcc
    19921 ggtctatccg gtgaaagggt attcggccac ggtgccgatc ctgcgcagcg atgccgcgcc
    19981 gcggcgcgcg atgatcgacg agtcgcgcgg catcgccttt tcgccgatgg gcgccgatct
    20041 gcgcatcacc ggcggcgccg agttcgcggg ctacggcaag gagcacgagc cgcacgacgt
    20101 cgcgcttctt tacgacacgg taaagcagct ctttcccggg gcgctggact acgcgcgcgc
    20161 ccgggtgcgc gcctgccagc gcccgatgac gccggagagc acgccgcgct tcggcaccgg
    20221 caagtaccag aacctgtggt tcaacatcgg cctcggtcat atgggcttcg ccatggcggc
    20281 cggcgccgca cgcatcacgg ccgaactcgt ctcgggccgc acggcgccga tcgatctcga
    20341 aggcctccgc atccgtcggc attgatgcgc atcttctttg ccgtgtggcc gccggccgag
    20401 accgcccacg ccctggcgca gtgggcgcgc gaggcgcagc gcggcaccgg cggcaagccg
    20461 acggacgagg cgaagatcca cctcacgctc gctttcttgg gagatgccga cgcacagcgt
    20521 gcaatcggcg ccgcgcgccg cgttacggca aatacccatg ggctgccgat cgagcaggcg
    20581 agctactggc gtcacaacca gatcgtctgg gtcggcccgc gcgagacccc gccgccgctg
    20641 caggccttgt tcgatcgtct cagcgaggag ctctaccggg aggagttcat cctcgagcgg
    20701 cggcccttcg ccgcgcacgt aacgctggtc cgcaaggcgc gcgccgccgc gctgccgccg
    20761 ctggcggcgc tcgactggcc ggtgcgcgaa ttcctgctgg tgcgctcttc gctctcgtcg
    20821 aagggctcga cctacgagcc gctggagcgt tttgccttga gctaggcccg cagggcgagc
    20881 cgggcgacga ggccgccgcc ctctcgtggc gcgagctcga gcgcgccgcc gtgcgcgcgc
    20941 gccacgcgct cgacgatcgc caggcccagg ccggcgcccg ccgggccgct gcgcgccgcg
    21001 tcgagccggg tgaaagggcg cttgaggcgc tcggtttcgg ccgccgggat tcccggaccg
    21061 cggtcgcgca cttcgattac cgccgagccg ggattcttct tcagctcgat ttcaacgggc
    21121 tctccagcgt agcgcagggc gttgtcgacg aggttgctca ccgcgcggcg tatcgccgtg
    21181 cgggcgaacc ggaacggcgc gacttcctcc ttcttgaggt gaacggcctt tccgagacgg
    21241 gtgtaatgct cggccacctc gtcgagcatc gtgccgagat cctggcgcga ctgctcctcg
    21301 ttcacgccgc gcgcgaagtc gaggaactgg ccgatgatgg cgtcgatctc gtcgatgtcg
    21361 gccactatcg tgtccgcggc tccggcctcg gcgccgctca tctcgagcgc gaggcgcatg
    21421 cgcgagagcg gggtgcgcag gtcgtgcgag atcccggcga gcaccatggc gcgctcgcgc
    21481 tccatgctct cgaggtcgcg cgccatgcgg ttgaacgccg cgccgaggct gcgcagctcg
    21541 cgcgcgcctt cctcggcgag gggctggtgc gcctcgccgc gcccgatgcg ggctgcggcg
    21601 cgcgtcagcg cggcgagcgg ccgggcgatg ctcgaggcga tgagccaagc gccggcgagc
    21661 gccagcgcga gcagcgccgc gccccatccc agccattcca ggccgaactc gagctcgaag
    21721 cgctcgcgcg gcagcatgac ccagaactcg tcctcgtcga tgaagaagct caccaggaag
    21781 ccttcgtcgc cgtcgcgcgc ataggcgaag cgggtttccg cgcccgtgac cgcccgaacc
    21841 ttccgcagga cgagattcag cagcggctcg tcgggcaccg gctcgagttt ctcgctcgcg
    21901 gtggcgggga acacgcgcag gccctcggtc tcgttcagct cgatcagcag ctcgcggcgc
    21961 aggaacgggt cggcgctgac cagcgccgcg cgcgtcaggt tgaccgtgct tacggtctgc
    22021 tgcgcgagct cgcgcgagcg cggctcgcgc tcgtagagcc ggtagatctg gaaggaggcg
    22081 agcaccgcgg tgacgatgag cagcgcaatc aggaggaacg accgggcgaa cagcgtcact
    22141 taggcctgat cgggcacgaa gacgtagccg aagccccaca ccgtctggat gtgcttcggg
    22201 tgcgaaggat cctgctcgac gatcttgcgc aggcgcgaga tctgcacgtc gatcgagcgg
    22261 tcgaacacct cgtactcgcg cccgcgcgcg agctccatca gcttgtcgcg cgacagcggc
    22321 tggcgcgggt gctgcaccag caccttgagc accgagaact cgccggtggt gagcgcgacc
    22381 tgcttgccgc cccgcgtcag cgtgcgcgtg gcgagattga gcgagaactc gccgaacgcg
    22441 aacacgccgc cctcggccgg cgctcccggc ggaccggcgg gagcgcgccg gcgcagcacc
    22501 gcgacgatgc gagcgagcag ctctctgggg ttgaatggct tgggcaggta gtcgtcggcc
    22561 cccatttcca gcccgacgat gcggtcgacg tcgtcgccct tcgcggtgag catgatgatg
    22621 gcgggcgcgc tttgctgcga gcgtagccgg cggcagatgg acagcccgtc ctcgcccggc
    22681 agcatgaggt cgagcaccac caggtcgtag cggtcgcgcg cgagctgctt gtccatcgcg
    22741 cctgcatcgg gcaccgcttt cacgtcgtag ccttctccgc tgagatactt gaccagcagg
    22801 tcgcggtgac gctgatcgtc gtcgacgatg aggatgcgag tcttgagttc cggcatgggc
    22861 aatagattgt tacaaaacga cagccggccg caaggctctc cgcgtaacat ggcgccccat
    22921 gatgctgcgt gccgcattgg cgttgatact cgccctgagc gctgtcgacg gctccgctca
    22981 gtccggcaag cgccacgacg gcggccgcga ccggcagatg agccaggaag agcgccagcg
    23041 catgcgcgag gacgtgcgcg atgcctatcg cgagcgccag gaccgccccc agcagaaagc
    23101 gcgaccgcag cagatgtcgc ccgaagagcg cggccggctg cgtcgcgaca tcgaggatgc
    23161 gaaccgggat ctgaagcgct agctcgagcc tgattcccgc gcctggcgcg ccggatcgtt
    23221 ggccgcgggt ccggaggagt aaaacctgca cgtattcttg cccgcctgct tcgccgcgta
    23281 catggcgatg tcggcgtgct tcacaagcgc ggtcgcgtcc gcggcgtcgt cgggaaagat
    23341 gctgataccg atgctcgcgg tgacggtggc gcgcgcgccc ggcagggtat agggccgcac
    23401 ggtctcgacg agcagcttct tcgcgacgat ctcgaccggc tcctggtcct gcacctcctc
    23461 gagcaccacc aggaactcgt cgccgccgag gcgcgcgatg aaatcgctcg cgcgcaggtt
    23521 gtcgcgcagg cgcccggcga gctcgacgag cagggcatcg ccggcgtcgt ggccgtaggc
    23581 gtcgttgatc gccttgaagc cgtcgaggtc gaggaacacc accgcgatct tcgagttgcg
    23641 gcggcgcgcg cgctgcaccg cctggtcgag cgccggcatc aggctggtgc ggttggcgag
    23701 gccggtgagc ggatcgctgt aggcgagcga ggcgatgcgc tcgcgcgcga gcgcgatctc
    23761 ggtgatgtcg cggcccaccc cgcggtagcc gaggaaggcg ccttgcgcgc cgaagcgcgg
    23821 atcgccgctg accgacaggt agcgcaccag gccgtcgggc atgcgccgcg caaactcgaa
    23881 atcccggaac ggcttgtgct ggtcgagctg cgcgcggtgc gccgcccagc cggcgtcgtc
    23941 gggactttcg cacggcagct cccacggcgt ttttccgatg gcgccgcgcc ccatctcggg
    24001 cggcaggtag ccggggccgt agacgagctg gacatagcgg tgcgcttcgt cggtctccca
    24061 gaagaagtcc gacgacatct gcgtgaggct gcggaagcgt gcctcgctct cgcgcagcga
    24121 cgcctctgcc tgcttgcgct gcaggaactg tccgacctgg ctgccgatga cgcgcgaagc
    24181 ggcgagcagg cgctggtcgg gctcgcgcgc cttcaggctg gagaagttga gcacgccgat
    24241 ggtcctgccc tcggcgatca cggcgaaggc aaatccgccg cgcagcccgg tgcccgtcca
    24301 gacgctgcgg tcgcgcacgc gcgggtcgcg cgtgatgtcg ggcgaccaga ccggctcgcc
    24361 ggacagccac accgtgccgg tcaggccctc gccggggcgg aagacgaagg tgcgcgagcg
    24421 ctcgacgaac ggcgcgatcg cgggatcggc gatggcccac gcgtcctgga agcgcagcag
    24481 gtcggggtcc gcgcggaagt atcggccgca ggcgaacccc tcggtttcgc acaccacccg
    24541 gatcacgttg cgcaggccgc cggcggcgtc ctcggcggcg gcgagcaggc gcgcgacctc
    24601 gtgctcgagc cgaaggatct gctcggcgcg cttctcctcg gtgacgtcgc gccccacgcc
    24661 gcggtatccg gcaaagctgc cgtcggcgtt gaacaccgcc tcgccgctga cggcgacgta
    24721 gcgcaacgag ccgtcggaca tggtgcgcca catgaggagg tcatggaacg gcttgcgcgc
    24781 ctcgagcgtg gcccggtgcg catcccagcc accctcgagg cccaggccgc tttcccagcg
    24841 gcgctgcccg agcaggcggc gttgcagatc cgggtcgccg ccggccacct gccgcccttc
    24901 gagccgcgtg aagcggaact cggcatcctg ctcccagtac cagtccgagg aaagatgggt
    24961 cagcgcgcgc cagcgcgctt cgctctcgcg caccgcgcgc tcggcctggg cgcggccgag
    25021 gaactggccg atctgcgtgc cgatcgcgcc cagcgtctgc cacaggcggt tgtcgggctg
    25081 gcggatgcgc cgcgaggtga actcgagcac cgccgcaatg cgctcgccgg cgcggatcgg
    25141 gaagatcgcc gcggcacgca acccggtctc ctgcgcgatc gcgcggcgca gcgcgcgcgg
    25201 gtcgctcgtt gcatccggga cccacaccgg gtcgcccgcc tgccacaccg tgccgaccaa
    25261 tccttcgccg ggggcgaacg cgaggtcgcc cgaggcctcg acgaaacggg cggcggcggc
    25321 gtcgccgggc gtggaccagt gggagatgcg gcgcagcacg ccggccggct cgtcgggcgt
    25381 ccagagctgc gagctgtccc agcgcagcga gtcgcagatc gcctcgagcg cgccgttcag
    25441 cgcctgcggc gcgccccgcc cctcggcgag gcgcagcgtc accgcgtggt cgagcttgag
    25501 gagctgctgg atgcgcttct gcttggtgat gtcgcggccg atgccgcgat agccggtgaa
    25561 acggccgcgg gcgtcgaacg tcggctcgcc gctgacgctg atgtagcggc gcgcgccgtc
    25621 gggcaggttg cgccacatca gcacatcgcg gaacggcgcg cgcgcttcga gcatcgcgcg
    25681 gtgcgcatcc cagccgcctt cgatctcgac cccggtctcc cagcgcttct tgccgaggat
    25741 gcgctgcgct aggccctgct cgggggccga gtccttgcga acctcgacgc gggtgaagcg
    25801 cagctcggcg tcctgctccc agtaccagtc ggaagtgagg ccgaggaagg aaaaatgcgg
    25861 ggcaggcgcg gcggcagcgg gttgccgccg tgcggatgag cgtgctgccg gcgcgcgctt
    25921 cttcgccgct ttcgccacag gcccccttcc ttccccgcag tcagcctact ggacgtttat
    25981 ccgcgcgtcc agcaactaga tcagatgtgc gatcgaattc gcatagtagg caagaagcgg
    26041 caattcttcc tgccgcgcgc gaaatagtcc ctgctcttcc tcgacgaggc gacgcaggcg
    26101 cagcatgcgc aggccgacgc tcagggcgta gtcgaggtcg ccgcgcggga tgtagacatg
    26161 cgcgcccgcg gcctgcaggc gcgcgagcag ccgcccgacc tcggccttga gctcgagctc
    26221 ggaaagcgcg cgttgcggct gcgaaacgaa cacggctgcg atcagcggca ccggcaccac
    26281 cggcacgatg cggccgacgg cttgcatcag ctcgattccg agggcagcca ccgccgcgcg
    26341 cctttcttcc cccgacagcg cctggtagtc gatgccccgc tcccgcatcg agatcggcgt
    26401 tccgaagttg acgcacgcgt agccgaagcg gtgccactcg ctgcgcagca tcagcgacgc
    26461 gttatgcagg aggaagcgga aggtgatcca gaggctcttc gctctacccg tcgagccttt
    26521 ttctttcaga agcgatcgat cttccagcac ccggtcgtag ttgagcccga gcggcacgaa
    26581 caccaggtcg cggtcgccgg cgaggcgaaa gccgcgcagc atgtagtcga gcacgccgag
    26641 cttgagctcg cgcatgcggc cgtcccgcgt tagcccgccc tccggaaaca ccgcctgcgg
    26701 cacaccggcc tcggtcgcca tggcgatgta gcgctccagc acgcgacggt agagctcgtc
    26761 gcgcgaattg cgccgcacga agtaggcccc catcgcgcgg atcagcgccg acagcggcca
    26821 gatgcgcgcc cattcgccca ccgcgtagga cagcgcagcc tggtcggcgg cgaggaaccc
    26881 ggcgagcacg tagtccatgt tcgagcggtg gttcatgaca cagaccaccg tcgcgctggg
    26941 gtcgatgcgc tcgaggccgg ggccatcgac gtagcccacg cgcacgcggt agaggagccg
    27001 cgcgacgcgg cggccgagcc agtagccggt gcggaaatag aggtaggcat tgaacgccgg
    27061 cacgatctcg cgggcatagc tctcgatttt cttgaaggcg gtaccgatgg ggatcttgtt
    27121 tgcaagagca aactgctcgg cggcctcctg caccttcggg tcggtcagca gccggtggat
    27181 caaggcctgg cgccgggtgc gctggaacgg gcggatgccg atgcgcagcc gggtcgagac
    27241 gtcttcgatc acccggtccg ccggatgcac cagcatccag cgcagcgccg ggatcaggac
    27301 atgctcgtac agcgcccaca gcgcgaacag cccgaccgcg agggcgagcc agagcggcag
    27361 cgcgacgagc tcggtcattt tcgctctcgc gagaagagat acagcccgct cgcgatgacg
    27421 atcggcgcgc cgatccacac ccccggcccc ggcacgtccc cgaacacgag atagccgaac
    27481 gccgccatgt agatcacctg ctgatagaga aacggcgcaa tcacactgga cggagcatag
    27541 cggtgcgcca gcgcgagcag gtagtggccg cagccgccca gcacgccgag caggcaggcc
    27601 acggtccatt cgagccagcc gctcggcgac tcccacgagg caagcgcgaa cggagcgagc
    27661 ccgatcgccg cgccgaccgc gggcaggtac tggatcgtct cgggcgaatc gtaggccgcg
    27721 agccggcgcg tcatcatcag gaatccggcg tacaggatcg cattgaccac ggcggcgagc
    27781 atcgccgggt ggaactccgc gccccacggg cgcacgatga cgagcacgcc ggcgaacccg
    27841 gcggcgatcg cgatccatcg cccggcgtcg agcttttctc ccaggagcgc cgcgctggcg
    27901 agcgcgatca tgagcggcac ggagaagaag atcgacgagg tgacggtgag ctgcaggtac
    27961 tgcagcgccc agaagttgat gccggtcatc gcgatgaaca tcgccgcgcg cagcatgtgc
    28021 caccgccaat gggtggtctt gaccagggaa acgcctttga ccgggaagag cacgatgccc
    28081 gcgaacaccg cgtgcgtggc gaatcgcagc cagaccacca tgatcacggg catgcccccg
    28141 accagccact tggcgctgcc gtcgagcagc gagaacagca ggtagcacag cgagaccagg
    28201 ccgatgccga tcagccggtt gcgtgcgctg tcagcgtgaa gcaagacccg ccttgtagtc
    28261 ctcgaggggg aactcgctgc cgtcggcatt ctccagccgc caaccctcat ccttgaggtt
    28321 gtcgagcagc ttgagcacca gggcgagggc gagccacacg gcatggaacg aatcggtgcc
    28381 gcgcacgtct tcgggttgcg tttcgagcgg cgcgatggcg attgcacagg cccagcggcc
    28441 gtcgcgcgct tccttcggcc gtccgacctc cgccatgagc ggcacgcgct cgccgtccgg
    28501 gcgcacgccg atgacctcga gcttggcgac gaggttcttc accagggaat ctgcgagccg
    28561 tcgtgcgaga agaattttcc ggaatcggcc gtgcgcagct ccgcgatgac cttgcgcagg
    28621 ccctggaccg acttctccgg cgtcagcggc gcctgcggcc cgcccatgtc ggtacgcacc
    28681 cagccggggc tgagcgccac cacgacgatg ccccgcgagg cgagcgttgc agcgaggccc
    28741 ttgaccagca tgttgagcgc cgccttgctc gtcgagtagg gaagcgtcat gccgctcgac
    28801 tcgctgatcg agcccaggcg gctcgacatc atcgcgatcg tcttgcggcc gctcgccgcc
    28861 acgttgtcga gcagagcctc ggccaccgcc gccgcgccga gcacgttcac ccgcaggatt
    28921 tcttcccacg actcgaaatc gaagctgccg ggcgccatgc ccttcctgcc ggtgatgccg
    28981 gcgttgcaga acagcagatc cagcggttcg cccttcaggc gcgcggcgaa ctcgcgaatc
    29041 tgcctccgat tcgtcatgtc catgtcgcgg cgctcggtgc cgatgacctt ccagccctcg
    29101 gccgcgtatt gccgcgccag ctccttgccc aggccgcggc cggctcccgt gatcagcgcg
    29161 gtcggcatgt caatcgagct tcccgattcc ggggagcagc ctgcccacgc ggcgcgcgta
    29221 ctgcagatac tgctcgccgt aagtgcgcag gaggtagctt tcctcctcgg cgatctgctg
    29281 ccgtatcgcg atgcacgcac cgaccagaat cagcgcggac agcatcgttg gcaggaggag
    29341 ggtatagccg gcgagaaccg cgatcagcgc caggaagatc gggttgcggc tgaaccggta
    29401 cagcccgccc gtgatcagcc ccggccgggc gcgctcttcg atgccgatgc gccacgaagc
    29461 gccgaggtcg agcatcgccg ccgcctggag gatcaatccg ccgaatagca gcaccgcgcc
    29521 caatgcgtgg cggacgcctg gcgccgcccg caggtccgcc tcggagagcg gtagcgattc
    29581 cggcgctccg gccgcaacga ccgcctggcc tacgagcagc accggcagca cgaacagcat
    29641 gccatcgcgt actttctgcg cgcggcttcc cttgaaaagc acaacgcccc acgttccgtg
    29701 gcggcgccgc tgcagccacg gccgccatac gaatacgatc gcaaacagga tcagggtgcc
    29761 ggcgagcggc aggtatctaa agtgctgcaa gcaccgcctc agtcacatcg cgcgtgcgtg
    29821 cggctccgcc gaggtccgga gtgaagatct tcttcgccgt gacggcctcg atcgccttca
    29881 tcagccgcgc ggcggctttc gcctcgccca ggtgctcgag catcatcgag gcggtccaga
    29941 aggtggcgat cgggttggcg atgcccttgc cggtgatgtc gaacgccgag ccgtggatcg
    30001 gctcgaacat cgacggaaac ttgcgctcgg ggttcaggtt cgccgtcggc gcgatgccga
    30061 gcgagcccga cagcgcggcg gccaggtccg acaggatgtc ggcgtgcaga ttggaagcca
    30121 ccatcacatc gatcgcggca ggtttcagca ccatgcgcgt cgtcgcggca tcgaccagga
    30181 tgcggtcggt cttgacctcc ggaaactcct tcgcgacggc gtagaagatc tcgtcccaca
    30241 gcaccatgcc gtggcgctgc gcgttcgact tggtgaccag cgtcaggtgc ttgcgcggcc
    30301 gcttgtgcgc cagctcgaat gcaaagcgat ggacgcgctc gacgcctttg cgggtgaata
    30361 ccgaggtctc ggtggcgatc tcgtccggta gtccctggtg gacgcggccg cccgagccgg
    30421 agtactcgcc ttcggtgttc tcgcgcacga tgacccaatc gatctcgcct gcgttcttca
    30481 tcggggaaga gatccccgga aggagccgcg agggcctcac gttcgcgtac tggtcgaagc
    30541 cctggcagat cgccagccgc agctcccaga gcgagacatg gtccggcacg tcgggcgcgc
    30601 cgaccgcgcc aaagaagatg gcgtcgaact tcttcagctg ctcgagcccg cctggcggga
    30661 tgtatttccc ggtttcgaga tagcgcttcg agctccaatc gaataccttg gcgtcaatcc
    30721 ggaacttttc cttcgaggaa agcgcctgca agacctccat gcccgcggga atgacctcgt
    30781 ttccgatgcc gtctccagga atggcggcga ttctgtaggt gttcacgtat cgcgatgaga
    30841 agccagggcg caaccggatc ttatcttgca gcgttgctcg gcggcgcggt gctgctcgcc
    30901 gcctgcggtc cgagcggcga ttcctccact ggggcgccgc cgggggagcc gccagtgctc
    30961 accgcgcagc gggtatttcc ggcgctgagc tttgcccagc cggtggcgct gctccaggcg
    31021 cctgccgacg gcacgcgttg gtttgtggtg gagcaggccg ggcgcgtgcg cgtgttcgcc
    31081 aacatttcgc aggccgctgt gttcgatacc ttcgtcgaca tcagcgcgcg ggtgacctcg
    31141 ggcggcgaaa tggggctcct cggcatggcc ttccacccga atttcccggc gaatccgcgc
    31201 gtctatctgt cgtataccaa tgaaactgcg ggaatccgtg tttcacgtat ctccgaattc
    31261 cgcctggatg tcagcggcga cctcgaccct ggctcggaga tcgtgctgct gagcatcggc
    31321 cagccggaga ccaaccacaa cggtgggcag atcgccttcg gccccgacgg gttcttgtat
    31381 atcggcatcg gtgacggtgg cggcggggat gaccagcacg gcgcgattgg caacggtcag
    31441 ctgatgacta cgcttctggg caagatgctt cgcatcagcg tgacgccggg aatctactcc
    31501 atcccgcccg gcaacccgtt cgccgccaat cccccgtgcg gaatgggtgg caccgggacg
    31561 ctgccttgcc cggagatcta cgcgtcggga ttccgcaatc cgtggcgctg gagcttcgac
    31621 cggagaaccg gtgcgttgtg ggtgggcgat gtcggccagg gcgcgctcga agagatcgat
    31681 cgcgtgacgc tcggcggcaa ctacggctgg cgatgccgcg agggcacgcg cccgaccgga
    31741 cttgcttgcg gctcggagca gggcctgctg ccgccggtcg ccgaatatgg ccgcaccgaa
    31801 ggcagttcgg ttacgggcgg ctacgtctat cgcggcagcg cgatcgcgga cctgccgggt
    31861 cgttacgtat tcggggactt cgtgaccggc agcctgtggc acattgccgg cgaaacatcg
    31921 ccgaccaggg tgatgcgtgc cggatttcca accgggctca acatcgcctc gttcggtgaa
    31981 gatgtcgccg gcgagctatt tatcgtcgac tacggcggcg ggatctaccg cttgaggtga
    32041 gctaggcggc gacgggcgag gcgctgcgaa ccgcctccgc gactgcggcg ccgacctcgg
    32101 aagtcgaggc cttgcctttc atgtccggcg tgcgcgggcc gtcgacgatc acgcgctcga
    32161 tgctgcgcac gaggtcggcg gcggcctcgg cgtggccgag gtgctcgagc atcagcgcgc
    32221 ccgaccagat cgcgccgatc gggttggcga tcttcttgcc cgcgatgtcg ggcgccgagc
    32281 cgtgcaccgg ctcgaagatc gacggcgcgg tgcgctcggg attgatgttg ccggaggcgg
    32341 cgatgccgat ggtgccggcg gtcgccggcc caagatccga caggatgtcg ccgaacaggt
    32401 tggaggcgac gatgacgtcg aagcggtccg ggttgagcac catctggatg ctcaggccgt
    32461 cgacgtggta ctggtcgctc ctcacgtccg gatatttctt cttcatctcg gcgaagcgct
    32521 cgtcccagta cggcatggtg atggtgatgc cgttcgactt ggtgcacgag gtgagatgct
    32581 tcttgcgcgt gcgggcgagc tcgaacgagt acttcatgat gcggtcgatt cccttgcgcg
    32641 tgaacacgga ttgctgcatc acggcttcgg atgcagttcc ttcaaaaatc ctacccccca
    32701 cgtttccgta ctccccctcc gtgttctcgc ggaccacgac gtagtcgatg tcgccgggct
    32761 tgcggccggc gagcgggcag ggcaggcctt cgaacaggcg caccggccga agattcacgt
    32821 agaggtcgaa ccagcggcga aagttgatca gcaggcccca cgccgagatg tggtcgggga
    32881 cgatcgccga attgccggcg gcgccgaaat agatcgcgtc gaagcgccgc aggatgtcgg
    32941 gggcgtcgtc ggggcacatc ttgccgtgcg ccttgtacca gtcgcagctc cagggaaact
    33001 cctgccactt gaagcgcagc ccgtgccttg cgcccaccgc ctcgagcgcg cgcacgccct
    33061 cgggcatgac ttcgcgcccg atcccgtcgc ccgggatcac cgcaatcgaa tgttctttca
    33121 tgcgccccag tatatagaat cgccgcatgc ggttcgcggc gatcgagacc tcgaccgagt
    33181 ggtgctcgct cgccgtctgg cgcgacggcg agatcgccgc gctcgagcgg cgcgccggcc
    33241 accggcattc ggagctcgcg ctgcccatgc tggaaacgct tctgggaaaa gacaagctgg
    33301 acgcggtggc cttcggcgcc ggccccgggg cattcaccgg gctgcgcatc gcttgcgcgc
    33361 tggcgcaggg cctcgccttc gcgcgcggcc tgccggtcat cggcatctct tcgctcgagg
    33421 cgctggcgca ggaatccggc gccgcgcggg tcgtggcctg catcgatgcc cggatgcgcg
    33481 aagtgtatta tgcggcgctt gaaaagcgcg ccggacgctg gcacgaggtg atcgccgcgc
    33541 agtgcgtcgc gccgcagtct gcgccgcgcc cgccgggaga agggtgggtg ggctgcggga
    33601 acggcttcga ggtctacggc gaagcgttga aggctaaagt ctcggtcatg aaacctgtga
    33661 tccatcccac cgccgttgcg gtggcgcagc tcgcggcgcc gcgcctcgcc gccggagaag
    33721 gcgtggacgc ggcgcttgcc gcaccggtct acgtgcgcga caaggtcgct ttcaccaagg
    33781 acgagcttga gcgccgttct taagaacgtt ccggagcttt cgctcatgcg cgagcgcgac
    33841 ctcgaagagg tgatggcgat cgaaagcgcc atctatagcc acccgtggac gcgcggcaat
    33901 ttcggagact cgctgcgcgc aggctacgag tgccgcgtgc tgaggctcgg cggggagctg
    33961 ctcggctatt tcgtgctgat ggtcgccgcc ggggaggcgc acctgctcaa cctttccatc
    34021 accgccgtgc gccagcgcgc cggccacggt agcggccttc tccgcgaagc catggcgctc
    34081 gcgcgcaatc tcggcgcgaa cagcctgttc ctcgaggtgc ggccgagcaa ccgcgccgcg
    34141 caggcgctct atacgcgctt tggcttccgc aagatcgcgg tgcgccgcgg ctactatccg
    34201 gcgcacgcgg gccgcgagga cgcgctggtt ctcaccctgc cgctcgcgtg agccggcgcg
    34261 ccgaggtcct ggccgagatg ggcatcaagc ccgtctggcg cttgaaagaa gagaaatcca
    34321 tcgaagaaag gaaatccatc gacgaatgga ttcccctgaa agccgccgtc tccggttgca
    34381 cgaagtgcgg cctgcacaag agccgcacgc agacggtgtt cggggtgggc gacgagagcg
    34441 ccgagtggat gctgatcggc gaggcgcccg gcgccgagga ggaccggctg ggcgacccgt
    34501 tcgtcggcca ggcggggaag ctcctcgaca acatgctcgc cgcgatcggc ctgtcgcggc
    34561 gcaagaacgt ctacatcgcc aacgtgctca agtgccggcc gccggggaac cgcaacccga
    34621 cgccggaaga ggtcgcgcaa tgctcgccgc acttgctgaa gcagatcgaa ctcatccggc
    34681 ccaagctgat cgtggcgatg gggcgcttcg cggcgcagac cctgctcgag agcgactcct
    34741 ccatcgcgag cctgcgcggg cgcgtgcatc gctacgccgg cgtgccgctc atcgtcacct
    34801 atcaccccgc ctatctgctc aggaccctcg aggacaaggc gaaggcctgg caagacctcg
    34861 tgttcgcgcg caggacgatg acggggctat gaagaaaaga gggacaagcc acctttcggg
    34921 ccagcgcgca gcttcgggga gcgggggccg gccttgcgcg agcgtggctg tccctgagag
    34981 cggttacgtc ccccttcccc acaggccgcg ctgaggctgg gcctcagccg caagggaaat
    35041 caagaggaca ggcaaagcca gaacctgctt catgggaact cctcaacccg cggaacagcc
    35101 ggccgggcga ctttattccg gacttatttt cccggcttgt gcgggtcttc ggcgcgcgcg
    35161 aaagcgagca ggcggtcgat ttcctcgcgg tggcggcggc ggttgcgcag gtaccagatc
    35221 tgcaccacgg tcatggtgcc ggcgacgaac aggccgaaga tcagcaccac ggtatagatc
    35281 gaatagccga cgctcagcag cagcgagtag agcgcaatca tcaccaggat cgagctgttt
    35341 tcgttgaagt tctgcaccgc gatggagcgt cccgcgccga ccaggtggtg gccgcggtgc
    35401 tggaggagcg cgttcatcgg caccacgaag aagccggcga gcgcgccgac caccaccatc
    35461 agcgccaccg cgagccagac ttgcttgacc acgatcatca cgaggacgat cagccccatc
    35521 gcgatgccga gcgggatcac gcgcaccgag cggtcgagcg ccacccagag cgaggcgagc
    35581 accgcgccga gcgcgatgcc gaatgcggtg acgccctgca ggatcgccgc cttggaaagg
    35641 tcgaagccga gcgcggcgcg cgcccactcg atgaggatga actgcagggt cgcgcccgca
    35701 ccccagaaca gcgtcgtcac cgcgagcgag atctgcccga ggcgatcgcg ccacagatgg
    35761 ccgacgcagc gccagaaatc cttgagcgtg tcgagcggat gggcgggcag cttcttcagc
    35821 acgactccgg tcttcggcac ctcgaggttg aagagcgccg cgatcgcgta caccaccacg
    35881 atgagcgcga tcgccgcctc ggccggcgtg tcgatcgagg tctcgatgag cggcatgtcg
    35941 aagtgcagca ggacgccgga cacgcgcacg tggatcaaca ggcccccgag catggtgccg
    36001 aggatgatcg acgccacggt gaggccctcg atccagccgt tcgccaccac gagctgccgg
    36061 tgcggcaggt actccgtcag gatcccgtac ttcgccggcg agtacgcggc ggcgcccagg
    36121 ccgacgatcg cgtaggcgat gagcgggtcg acgccccaca gcatcgtcac gcagcccacg
    36181 atcttgatgg tgttcgcgat cagcatcacc tgccccttgg gcaggcggtc ggcgaaggcg
    36241 ccgacgaacg gcgcgagcgc cacgtaggag atgacgaaaa agaacttgag gtagggcgtg
    36301 agccacgacg gcgcctcggc ctggatcagc agcgcgatcg cggcgaccag cagcgcattg
    36361 tcggcgagcg acgagaagaa ctgcgccgcc atgatgatgt agaaaccgta attcaaatcg
    36421 aggccttgtc cccctcggca ggtctcttct tcgcgttttt tcgccgcggc gctttatagc
    36481 acaataaccc tccatgcccc ggccgatccg cgcgaccatt tccgccgcag cgctcgcaca
    36541 taatttgaca gtggcgaggc gccgggccgc gggcgccaag gtctgggcgg tggtcaaggc
    36601 gaacgcctat ggccacggca tcgagcgcgc ggcgcgcgcg ctggccgccg ccgacggctt
    36661 cgcggtgctc gacttccagg aagcgctgcg cctgcgcgcc gccggcgtgg cgcagccgat
    36721 cctgatgctc gaggggttct tcagcgccgc ggatctcgag cttctgccca agcacgcgct
    36781 gaccccggtg atccacaacc cggagcaggt cgagctgctc aggcgcagcc ccctcgccgg
    36841 cgagatcgac gtgtatctca aggtgaacag cggcatgaat cgcctgggct tcggcgtcga
    36901 gagcctgggc ccggcctaca actcggtgcg catgcacccg caggtgcgca acgtcacctt
    36961 catgacgcac ttcgccgacg ccgacggcgc cggcggcgtg caggcgcagc tcgagtggtt
    37021 cgacgagctg accaagtcct tcgaggtgca cagccgctcg ctcgccaact cggcggcgct
    37081 cctgcggttc ccggaagcgc gcggcgactg ggtgcgcccg ggcatcatgc tctacggctg
    37141 ctctcccttc tccggccaga gcgccgagca gctcggcctg aaaccggtga tgacgctgag
    37201 ctccgagatc atcgccgtcc agcagttgca gccgggcgag cgcgtggggt acgggttttc
    37261 gtacgaggcg gtcggggaga tgacgctcgg catcgtcgcc tgcggctacg ccgacgggta
    37321 tccgcgacac gcgtcgaccg gcacgccggt gctggtcaac ggcaagcgca cccgcctcgt
    37381 tggacgcgta tcgatggaca tgatcagcgt cgacctctcc ggactgcccg gggcgtacat
    37441 cggcacgccg gtcaccctgt ggggcgaggg gctctcggcc gacgaagtcg gcgccgcggc
    37501 cggcacgctg agttacgagc ttctttgcaa gctgagcacc cgcgtgccca tcgtcgaggt
    37561 gacttgaaaa aacctaaaac cgcgtacgtg tgctcggaat gcggcgcgac tgccctccag
    37621 tggttcggct cctgcccgtc gtgcggcacc gcggggacgc tgagcgagtc gatcgccgag
    37681 cgcggctccg gattccggcc gaagtccgcg cccgagacgg tcggcctggc cgaagttcag
    37741 ctcaaggacc tcgagcgcat cccgaccggg atcggcgagc tcgaccgcgc gctcggcggc
    37801 gggctggtgg cggggcaggt ggcgctgctc ggcggcgacc cggggatcgg caagtcgacc
    37861 ctgcttcttc aggccttgac cagcatggcc ggcaccggag cgctgatcta cgtgaccgga
    37921 gaagaatcgg tagagcaggt ggcgctgcgc gcgcggcgcc tcggcctcga agccgcgaac
    37981 gtccgcatcg ccgccgagac gcagctcgag cgcgtgctcg gcgcgctcga ggcggcgcgg
    38041 ccgcgcgtgg cggtgatcga ttcgatccag acgctctggt cggagagcct gcagtcggcg
    38101 ccgggctcgg tggcacaggt gcgcgaatgc gccgctcagc tcacgcgcca cgccaagcgc
    38161 gccggtacgg cgctgtttct ggtcggccac gtcaccaagg aaggcgcgat cgccgggccg
    38221 cgcgtgctcg agcacatcgt cgacacggtg ctctacttcg agggcgatgc gcactcgtcg
    38281 ttccggctgg tgcgcgcggc gaagaatcgc ttcggcgcgg tgaacgagct cggcgtcttc
    38341 gccatgaccg agaaggggct gaggggcgtt agcaacccat ccaaattgtt cttgtctgac
    38401 catgacaaac ccgttgcggg gtcctgcgtg ctcgccacgc tcgagggcac gcggccgttg
    38461 ctggtcgaga tccaggcgct ggtcgatccg gcgcacacgc cgaacgcgcg ccggctctcg
    38521 gtcggcctcg aggcgaaccg cctggccatg ctgctcgcgg tgctgcaccg gcacgcgggc
    38581 atcgccacct gggagcagga cgtcttcgtg aacgcggtcg gcggcgtgcg catcggcgag
    38641 ccggccgccg acctcgccgt ctgcctcgcg gtggtgtctt cgttttccga caagcccatc
    38701 tcgtccaggg tcgcggtgtt cggcgagatc ggcctcgcgg gcgaggtgcg ccccgccgtc
    38761 cgcggacagg agaggctcag ggaggcggcg aagctcggct tcgaaagagc gatcgttccg
    38821 aaggcgaacc tgccgaaggc gaaaatcgcc ggtctggaga cgctcgcggt agagcgtatc
    38881 gaccaggcgg ttcgcttact ccgggaactg tagtcgcgat cgtcctggcg atattcggac
    38941 tgctggggtt cacctcgagc tcgcgcgtgc gcgggaggat cacgagatcc atgtcttcca
    39001 tggcgatcca gccgacgagc gcctgatcgc ccatcaccag cgcgccgacg aaggccgcgc
    39061 ggttcccgaa gcggatttcg agcgggccga cgtagggcac gagcttcttc gacccgtcag
    39121 cgagcaggac ctcccgatcc gcaagcttct ccaggcgcag ttggtcgcag atgcgctgcg
    39181 gtatgcacac gtggagcgtg gcggagtcgg cgagcgcatc gacctccagc gcccgcgctg
    39241 gcgcacgtgc attccggaga acgatcttgt tcatgaccag gcggatcgta acgcgcgatc
    39301 cggattcgta ttaccggggt agtcgcgccg tcacactggt ggtggatttc tttccgggaa
    39361 cgtgccgcgc tggtgccagt acggatacgc gggcacttgc gcgctcgtcg cgtcgagctt
    39421 cgcgacttgc tccgcggtga gcttccagcc gaccgcgccg aggttctgcc tgagctgctc
    39481 ctcgttgcgc gcgccgatga tcaccgtgga caccgacggc cggccgagca cccagttgag
    39541 cgcgacctgc ggcaccgtct ttcccgtttc cttcgccacc gcatcgagag cgtcgaccac
    39601 gcggtagaga tattcgtccg gcaccggcgg ccctttgtcg atcgcgagct ggctctgcag
    39661 ccggctcact ttgggcagcg gatggccgcg gcggatcttg ccggtgaggc ggccccatcc
    39721 gaggggactc cagaccaccg cgccgacctt ctgatcgagc gcgagcggca tcagctccca
    39781 ctcgtagtcg cggccgatga gcgagtagta cgcctggtgg gcgacgtagc gcgcaaggcc
    39841 gtacttctcg gagaccgcta gcgacttcat caggtgccag ccggagaagt tcgagcagcc
    39901 gatgtagcgg agcttgcccg cacgcaccag atcatcgagg gcgccgagcg cttcctcgac
    39961 cggcgtgaga gcgtcgaagg cgtgcagctg gtagaggtcg atgtaatccg tgccgagacg
    40021 ccgcaggctc gcgtcgaccg cgcgaggcag atagtggcgc gaggagccga cgtcgttcgg
    40081 ccccgggccg cggcggaacg agccttttgt ggaaatgagc actttgtcgc gccggccctt
    40141 gatcgcctgg ccgaggatct cctccgcctt tccattcgag tagccgtcgg ccgagtcaaa
    40201 catgttcaaa ccactttcca aacaaacatc aatcaggcgc gtggcctctt ggacgtcgct
    40261 cgcgccccag gccttgaaga actcgttggc gccgccgaag gtgccggtgc cgaaggtcag
    40321 cgcgggcacc atgaagcccg agccgccgag ttgccgttgt tccatccgca gttctcctta
    40381 gctttccttg aggatcatgc ttgccgcgcg cacgccgctg cgcaccgcgg cctccagcgt
    40441 cggcggatat tctgcatcgg tgtagtcgcc cgccaggaat actcccgcgg tttggatctc
    40501 gggtctcttc aagccgggcg tgcaggcgat agtcgcgcgc ttttcggcga tgacctgcga
    40561 ccacagtgga gaaggcaagg cgggaagcgc atgccgcagc tcgcgatggc aggtctccgc
    40621 gagctcgtcg tgagtcatct gctggtgatc gccctgggcg ctgatcacgc aggcgagccg
    40681 gcctttctcg ccgagcagcg tgccgcggtc gaagacccac tgcaccaggc cttcgcccat
    40741 gccgagcatc gggaagtcga ggcgcgtgct ttccggatac tgcaggtagc aggtatagat
    40801 cggctgatag gtgtagccgt tcgccgcttc cggcagcaga gttttcagct ggtgtggccc
    40861 gacggccacg acgacattgg aaaagctggc tttcaaggag tccagttccc ttacggtagt
    40921 cccatagcgc acctcgccgc cgcgcgcgcg aacgaactcg gctgcaggct cgggaaacaa
    40981 ccgcgacagg tcgacgcgcg gcaggagcaa atcgctcgcg tcgcggtcgc ccgcaagagt
    41041 atcgcgcagc gcggcgagga agacgttggc ggaggcctgg tcgagcggcg tattgagcgc
    41101 cgagacgcaa agcggcgccc acaggtagtg gcgcatcctt ccacgctggc cgtgggcgtc
    41161 gagcagcgcc tggaccgtga tatcgggctg catgcgccag ttgcgccggc gcagcgtcgc
    41221 gacgaaccta agtgtgccga ggcgctcggt gaagggcacc ccgcgggcga gcagcaggcc
    41281 cgccagccgc ccgaatggcg ctgcgcggaa cgcgaagccg ttcgcgtagc ggatttccag
    41341 gggaacgcgc agcaacgcgt ctggcgggac gccgacggtg cgcatcaggc cgaagagctg
    41401 cgaatacgcg ccgacgaaga tgtgctggcc gttgtcgagc gtgtgacctt gggagatcac
    41461 gcggcgcgca cggccaccgg gcaccggacc ggactcgaac accgtgattg gcacgccgcg
    41521 ctcagcgagc gtgaccgccg cggccattcc cgcataaccc gctccaacga tggctacgcg
    41581 gtcacccacg ttttccaggc gagccagaat ttccgaaggg gagtcagaga agttcttccc
    41641 gtgaggacac ggaagccgtc gcgctcgatc tcctccagca gcgtgcggta gatcgccgcc
    41701 atgatcagcc cgggccgctg cgctttgcgg tcggccgcgg ggagggcttg catcgctttc
    41761 tcatagtgcg agcgagcgcg cgcggcctcg aacgccatca ggcggttgaa ctccggcgtc
    41821 tcgcgcgttt gcaagatgtc ggctgccggg acgccgaact gcttgagatc cgccgccggc
    41881 aggtagatgc ggttcttgcg cgcgtcctcg cccacgtcgc ggatgatgtt ggtgagctgg
    41941 aacgcgatgc ccagatcgcg cgcgtattcg agcgtgcgcg ggtcgcggta gccgaagatg
    42001 ccggcggcga gcagtccgac cacgcccgcc acgcggtagc agtagcgctc gaggccgggc
    42061 cagtcgagat agcgcgtctg cgtgaggtcc atctccatgc cgtcgatgat gtcgttcagg
    42121 cgctttgcat cgatggaaaa tcgttccttg taaacaccca gcgccttcgt caccgggtgc
    42181 tgcgggttgc cggcgtcgag gtttgcgacc tccgagcgcc accaggccag cttcgccgcc
    42241 gcgatctgca tctcgccggt ctcgtcgacg acgtcgtcga cttcccggca gaacgcgtac
    42301 agcgcggtaa tcgcgcggcg ccgctcggag ggcaggaaca ggaacgagta atagaagctc
    42361 gacccgctgg cagcggcctt ctgctggcag tattggtcgg gcgtcatgcg agaaacgggg
    42421 acagaccacg gtttccccac gcgtttatgg cgaggttcaa aacgtggtct gtccctgatt
    42481 tctggtcagt cataccgctc ggatcagaag caagggccag tcgaatgcac gaagcgtcgg
    42541 gcggcgccgg aatacgtcgt agcgggcgcg ctcgatcttc tccaggatgc gcagtccgcc
    42601 ctggatggtc gcgcggatct cgaggccgat gcggccgggc agggcgcgtc ccaggttctt
    42661 gccggacagc atcagctctc gtgctctctc gacctggaac ttcatcaggc tgcgccacgc
    42721 gtcgtcgcag atcttctgcg aaatgtgccg ctcggtcacg ccatggcgcg ccatctcgtc
    42781 ctgcggcagg taaatgcgtc cgtcgttgag gtagtcgacg tccacgtcct gccagaagtt
    42841 gacgagctgc agggccgagc agatggcgtc cgaggcccgg caatttgaat cactcgttcg
    42901 tttgaaaagc tgcagcagca gccggcccac cggattcgcc gaacgccggc agtaatccag
    42961 gacgtcggca aaatttgcat agcgcttctt cgtcacgtcc tgcgagaacg catccagcag
    43021 gtcggcgaac agctcgaccg gaagctcatg ttcgcgaatg atttttgcga catcccgaag
    43081 gagcggatcc tcgggcgttt ccagctgcct gcggtagtcg tttaacttcc ggaggcgtaa
    43141 ttcgggggga tcgtcgcctt cgtcggcgaa gtcgtcggcg ctgcgcgcga agcggtaaat
    43201 gatctcgatt ggctcgcgca gcggcgcggg caagagcagc gaggcgacgg ggaagttctc
    43261 gtaatggccg accgccatca gggaataaga tctcagaggc agcggctata atacgttgat
    43321 tctttagcga aggtggcgga attggcagac gcaccagact taggatctga cgccgcaagg
    43381 catgagggtt cgagtccctc cct
//
LOCUS       DATBSZ010000137        32520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_156017, whole genome shotgun sequence.
ACCESSION   DATBSZ010000137 DATBSZ010000000
VERSION     DATBSZ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 32520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32520
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156017"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VIG70_08885"
     CDS             complement(<1..204)
                     /locus_tag="VIG70_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEY8250708.1"
                     /translation="MAVNLRAPSPAALLAVRGIELGVAMAGIKQPGRKDLLVMRLSPG
                     AAVAGVFTQNRFCAAPVVLARKHL"
     gene            complement(209..2971)
                     /gene="secA"
                     /locus_tag="VIG70_08890"
     CDS             complement(209..2971)
                     /gene="secA"
                     /locus_tag="VIG70_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY8250709.1"
                     /translation="MEFLRSNLEALWRLPARIFGSRNQRLLKQYSHAVVSINAFEPAL
                     EKLSDSQLKEKTSELKKRHAEGATLDELLPEAFAVVREASKRTLRMRHFDVQMLGGMV
                     LHNGKIAEMKTGEGKTLVATLPAYLNALTGKGVHIVTVNDYLANRDAEWMGKIFHFLG
                     LTVGVNLPHMEPEKKRAAYAADITYGTNNEFGFDYLRDNMAMHVEERFQRGLHYAIVD
                     EVDSILIDEARTPLIISGQAEDRTEIYLRMNEVAPLLTRQKEEKKPDQPEPPGDFYVD
                     EKNHQILLSEAGHQKAEEILVRKGLLPEGASLYEPAYINLVHQLYAALRAHYLFHRDQ
                     HYVVQNDEVIIVDEFTGRLMVGRRWSDGLHQAVEAKEKVQIQNENQTLASITFQNYFR
                     MYGKLAGMTGTADTEAYEFQQIYGLEVVVVPTHMKMVRADRQDQVYRTAKEKHQAVIN
                     DIRDCHDRGQPVLVGTTSIENSELLSGMLQKDKLPHQVLNAKQHAREAEIVAQAGRPK
                     MVTIATNMAGRGTDIVLGGNVEKQCDFVEANPDLDLNEKQQRIQTLRGEWQALHDQVV
                     KAGGLHIIGTERHESRRIDNQLRGRSGRQGDPGSSRFYMALEDPLLRIFAGERINRIM
                     VMLKMPEGEAIEHAMVTRSIESAQRKVEARNFDVRKQLLEYDDVANDQRKTIYALRNE
                     LLESQDVSPRIADLRAGVIGDLFRIYVPEESVEEQWDIAGLENALKAELQLELPLRQW
                     MEKEPELQPEQLVERIVAKADEAYHLKVPSGAESSFHQYERYVVLQILDAHWREHLAA
                     LDHLRQGIHLRGYAQKNPKQEYKREAFELFGVMVEAVKLEVVKTLTAVEIRAKEDVPQ
                     ADDKLHVQNVQLQHGEPAEGAGEGEAPEEKKAQPSVRHTQKVGRNDPCPCGSGKKYKH
                     CHGKLT"
     gene            complement(3015..3953)
                     /locus_tag="VIG70_08895"
     CDS             complement(3015..3953)
                     /locus_tag="VIG70_08895"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8250710.1"
                     /translation="MQIILISDRLARARSVHMSLRHLVGTAFAALLLLFVATATLYWL
                     TLRYAAEVRLPALQQLVAAAQEAEAERERVFVQQNLNSMAMKLGEMQAQLTRMDALGE
                     RLSALAGIKPQEFRAAEAPGMGGAAPTQMPPQNLSLGDFSEKLLVLARQVESRNDMLG
                     VLEAQLFEQAVKKKMIPTMMPVKASYNPSGFGRRIDPFNGQWAMHEGIDFIADYGAPI
                     VAAAGGVVIFAGFHPQYGYMVDIDHGNDLVTRYAHTSKIMVREGDLVQRGRKIGEVGS
                     TGRSTGPHLHFEVRFRGASQNPAKFLVPATTVARGK"
     gene            complement(4407..4589)
                     /locus_tag="VIG70_08900"
     CDS             complement(4407..4589)
                     /locus_tag="VIG70_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250711.1"
                     /translation="MFAVLRVAVVLVAIVLGVSMLAWLLTGDPRWRRITWVVFKYAVF
                     ALLAILVLFAGEALLE"
     gene            complement(4590..5504)
                     /gene="lpxC"
                     /locus_tag="VIG70_08905"
     CDS             complement(4590..5504)
                     /gene="lpxC"
                     /locus_tag="VIG70_08905"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY8250712.1"
                     /translation="MLRQRTLKSLIRASGVGLHTGQKVRIVLRPAPPDTGVVFRRIDL
                     PSPVDIPARAELVGEARLASTLVKDGVKVYTVEHLMSALSGLGVDNVFVDLDAPELPI
                     MDGSASPFVLLLQQAGLEEQAAPKRFLRVKKSVEVRDGDKWARLEPYQGFKLSFSIEF
                     RHPMIERSTQSVTVDFAESSYLKEIARARTFGFMHEVEDLKDSGFALGGGLENAVVLD
                     EQGVLNSDGLRFADEFIRHKLLDAIGDLYLAGRPLLGAFTAHKSGHGLNNKLVRAALA
                     DAGALETVVFERAEEAPAGVARLSTQLG"
     gene            complement(5525..6715)
                     /locus_tag="VIG70_08910"
     CDS             complement(5525..6715)
                     /locus_tag="VIG70_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8250713.1"
                     /translation="MAESAPSGEALFGAMFEQAAVAIALFSIGGRFLRANPAMCRLLG
                     YTEHELQQKTHLDVIHADDLEAAAVARAQVISGRMKPKVAERRYVHKDGSTIWVQMNG
                     LVVRDESGAPECTVLVASDISALKRSVRKSKQRFRRMVEMSSDWYWMQDDQFRFIEVS
                     GLEPELDSDVVIGKTRWEIPGLGALPEKVWEQHRAKLERHESFTDFVFLRYNKSGELR
                     YLSVSGEPLFDEKGKFIGYHGVGKDITERARDQKALEDSERRYRALFDVHPQPMWVVD
                     ANTFAFLAVNGAAMRLYGYSKEEFLTLTADQIRPEEDVDDLRRAFADWSNNYSQRIWR
                     HKKKSGEVIPVRVTSFNLEFAGRRARLGVIEDLSERLLAEERAKQSEQRYRALLKERG
                     GGDT"
     gene            complement(6792..7982)
                     /locus_tag="VIG70_08915"
     CDS             complement(6792..7982)
                     /locus_tag="VIG70_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY8250714.1"
                     /translation="MAEELFARGAFAAESYGELANGRVYKAGAMASPGKSLLVILAVL
                     LTSACTSMQTMYFGSSYQAIANANDPLLLRHSGAPAFEQTGDLVEKSPAMWNEGYALL
                     GYSQVVSPLLTSLAPDYSTKWGAELGAARVLMETPRPGESNLHYYLVTYWGRLDPGAF
                     SLGATLRDLPEEVLKLVGREMNLVMVGSVTPGTPAARAGLRQGDVVAGMDGERVPDAR
                     ALTERIAARRGTQAVLNVLRRGEEIDVPVALAAPAARGASRVQFPLAPWLDTPATDYS
                     SLSAANLASANMAATMKRVEQDRKAYEERSRAQLQRDIAALDARLDARQAASQDTSRR
                     GGSKPRPQLQQGYPTVVNSPEHYRKLMEAGSAATLRNIDGLVQMRQLFGELRPADPKM
                     FGGK"
     gene            8172..9221
                     /locus_tag="VIG70_08920"
     CDS             8172..9221
                     /locus_tag="VIG70_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250715.1"
                     /translation="MRMNVLPLSAALLLACAAPAFAAPGALDANASLPAVKRIAAEIA
                     ADLARVCPLAQPGDQAAFDRCRQALFDGSPLKRNMHSIVIWGRVKPGAALKETHLTQF
                     APDVLTGMYIPLFMFNGKHTIAFDERENLFVVRLEAGFRNRLQPGQFPYPFWHSDAKW
                     STYQAANNLLLWIDPKSVTIRYGQFTDHGTTAPISLSAPVKHEFDGKWMWTDAAGRVQ
                     PQATLFDGLFSPDNPYLHKLDGAYRKLAIGLREGQCSSCHDPDNKHDMKRLVLLQTPA
                     HAAGEIKRVIKALRRDSMPLDDAGIEQPLEGPVKEALLKHAGAFDALVDAAKEWERVH
                     AARNDEAGKRTAAVR"
     gene            9354..9749
                     /locus_tag="VIG70_08925"
     CDS             9354..9749
                     /locus_tag="VIG70_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8250716.1"
                     /translation="MKSAKWMVGAGLAALAAVTAYAQLPGITRTPVQEQDLGSLPGRQ
                     AVVARVEFAPGALVPKHTHAGEEIGYVVEGTIELIVEGQPPRIVKAGEGFLVPAGIVH
                     EGRNTGSDKAVVLATYVHEKGKPVATMVK"
     gene            complement(9820..10986)
                     /gene="ftsZ"
                     /locus_tag="VIG70_08930"
     CDS             complement(9820..10986)
                     /gene="ftsZ"
                     /locus_tag="VIG70_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY8250717.1"
                     /translation="MFEIVDSQATGPVIKVIGVGGAGGNAINHMIETGVQGVEFIAVN
                     TDAQVLARNKARNQLQLGSSGLGAGAKPEEARTVALAERERIEEAVAGSHMVFITAGM
                     GGGTGTGAAPVIAEVARGLGILTVAVVTKPFTFEGSKRMKIAEAGIEALQPHVDSLIV
                     ILNDKLEEVMGEDVTQEDAFRAADDVLNNAVAGIAEIINLPGLVNVDFQDVKTVMSEQ
                     GMAMMGSASASGIDRARIAAEQAIACPLLEGVNLAGARGVLVNITASRPSLKLRETKE
                     VMNIIRTFAAEDATIIYGGVYDDALAEDLRVTVVATGLGLAQAARQPKPQLVLNNVAA
                     QKTGTDNAHGPVDYQQLDQVPAVIRRNRASTVEALQQSGVDKYDIPAFLRKQAD"
     gene            complement(11017..12246)
                     /gene="ftsA"
                     /locus_tag="VIG70_08935"
     CDS             complement(11017..12246)
                     /gene="ftsA"
                     /locus_tag="VIG70_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY8250718.1"
                     /translation="MPKENKNLIVALDIGTSKVACLVGELGADGALAMLGMGSHESRG
                     LKKGVVVNIEATVAAIQRALEEAELMADCKIASAFVGIAGNHIRSFNSTGMVAVKDRE
                     VSALDVNRALDTARAVNIPTDQQILHVLRQEFIIDGQEDVREPIGMSGVRLEVKVHIV
                     TGAVSAAQNIVKCVRRCGLEVSDLILQPLASSRAVLSEDEKDLGVCLLDIGGGTTDIA
                     VFTHGAIRHTAVIPIAGDQITNDIAMALRTPTADAENIKIRHGVALRNLADPGESIEV
                     PGIGERAPRSMSRQTLAEVIEPRVEELYSLVQQVLRESGFEELLSSGVVLTGGSAVMQ
                     GMVELGEEIFHMPVRVGIPRYAGALAEVLRTPRHATGVGLLYEGMAQMEQGKLSRQSG
                     SVKAVLGRMREWFQRNF"
     gene            complement(12252..12938)
                     /locus_tag="VIG70_08940"
     CDS             complement(12252..12938)
                     /locus_tag="VIG70_08940"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY8250719.1"
                     /translation="MSLFFGILAGLLMLSACAGALYWLLAPERFPVSEVVLKGNLKHT
                     TAAEIEAALPRASGNFFAVDLANARAGVERLPWARRVAVRRVWPNRLEVWVEEHIALA
                     RWGDDALVNTHGERFVAKTKEPLPAFIAPGGTSAEVTRRYRRFAEIVAPLGTGVERVV
                     LTPRHAWQLRLGNGLHLMLGRDADLAEERLRKFVAAHPATLARAGKKHEYVDLRYPNG
                     FALRVPDWNG"
     gene            complement(12940..13854)
                     /locus_tag="VIG70_08945"
     CDS             complement(12940..13854)
                     /locus_tag="VIG70_08945"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY8250720.1"
                     /translation="MTSHFGKVAVLLGGKSAEREVSLKSGGMVLAALRKKGVDAHPFD
                     PKDRDVQDLVKERFARVFIALHGRFGEDGTVQGVLEWLGIPYTGSGVLASALAMDKVR
                     TKRLWAAERLPTAPYEVLAGDSNLKAVAKKLGTPLFVKPASEGSSVGMTKVKKASGLE
                     EAYALAVNYDPIVIAEKFIDGPELTVAIVADQVLPIIRIETPREFYDYEAKYIANDTR
                     YLIPCGLSDKKEKEIQALCLKAFRALGCRGWGRVDLMLDTRGRPFLLEVNTSPGMTDH
                     SLVPMAARAVGMSYEDLCVKILELAHVG"
     gene            complement(13851..14762)
                     /gene="murB"
                     /locus_tag="VIG70_08950"
     CDS             complement(13851..14762)
                     /gene="murB"
                     /locus_tag="VIG70_08950"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY8250721.1"
                     /translation="MSEPIRFQKLRGELRRDEPMGRHVSWRTGGRADRCYAPADLHDL
                     GAFLRQLPEDEPLLFVGLGSNLLVRDGGWRGTAILMHAASHRPELRDGKIYAEAGVAS
                     PKVARFAAMHDLEGAEFLAGVPGTVGGALAMNAGCYGGETWDVVEHVATINRRGKMIV
                     RGRDEYDIGYRHCAPKSPGEEWFAAAWFRLKPGDGATSRAVMKELLARRIATQPLSLP
                     NAGSVFRNPPKHHAAHLIEACGLKGFERGGARVSEKHANFIVNPKGAAAASDIEWLIR
                     HVQRMVYQMKGVYLATEVRIVGEEASS"
     gene            complement(14765..16129)
                     /gene="murC"
                     /locus_tag="VIG70_08955"
     CDS             complement(14765..16129)
                     /gene="murC"
                     /locus_tag="VIG70_08955"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY8250722.1"
                     /translation="MKHKVRHIHFVGIGGSGMSGIAEVLANLGYQVSGSDLAENTATK
                     RLASLGAKISLNHKPQHINGADAVVVSSAVQADNPEVIAARERRIPVVPRALMLAELM
                     RLKQGVAIAGTHGKTTTTSLVASVLAEAGLDPTFVIGGRLNAAGSNARLGAGDFIVVE
                     ADESDASFLHLQPVIAVVTNIDADHMDTYQQDFGRLKQAFAQFLQNLPFYGAAVLCVD
                     DANVREILPEVTKPVLTYGTGEDASVRAEAIEHDGGRMRFRALRKGAAPLDVVLNLPG
                     RHNVLNALAAIAVGTEIGVKDASIQKALKEFRGVGRRFQNYGEIDGKFTLIDDYGHHP
                     AEMAATLEAARGAFPGRRIVLAFQPHRYTRTRDLFEDFVRVLSGVDVLLLAEVYAAGE
                     APIVAADGRSLARALRLAGKVEPLFVEDIAAMPDTIRRTAQEGDVVITMGAGSIGAVP
                     SQLI"
     gene            complement(16126..17178)
                     /gene="murG"
                     /locus_tag="VIG70_08960"
     CDS             complement(16126..17178)
                     /gene="murG"
                     /locus_tag="VIG70_08960"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY8250723.1"
                     /translation="MKSILIMAGGTGGHIFPGLAVAEQMRAAGWDVVWMGARGGMEER
                     LVPRHGYRTAWIRAKAARGKGLLQKLLLPANLLYSFWESARHIRAVRPSVVLGLGGYV
                     AFPGGMMASLLNRPLALHEQNAIAGLANRVLAQVSDKVMVAFPDALKGAEWTGNPVRA
                     EIAAIAPPEKRFESRHGQIRILVVGGSLGAQALNEALPKAISLLPQKPRVVHQAGEKH
                     LESLQANYLAARVEGELVAFIDDMARRYAEADLVICRAGAVTIAELSAGGMASILVPF
                     PHAVDDHQTANAKFLSDRGAAILIQQRDLSPEKLAALIRSLDRARLLDMARKARALGK
                     PDAARVVAQRCMEIAK"
     gene            complement(17175..18338)
                     /gene="ftsW"
                     /locus_tag="VIG70_08965"
     CDS             complement(17175..18338)
                     /gene="ftsW"
                     /locus_tag="VIG70_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY8250724.1"
                     /translation="MSQTYVFARNAPSAEYDRSATWAALLLAAIGLVMVYSASIATAE
                     ASRYTANNATWFLMRHAAYLGLALAAAITVFLVPARLWQKAAAWLFVAGVAGLALVLV
                     PGFGREVNGARRWLDLPGFGFQPSEAVKLAVVLYAADYTARKHAVLKSFRRGLLPMLA
                     VMLLVSWLLLREPDFGALVVIAVIAFSILFLGGMNGRHFAALLGMLAAGFALLVVFSP
                     YRMQRIFGFMDPWSDPFGKGYQLSHALIAFGRGEWLGVGLGASVEKLHYLPEAHTDFL
                     LAVIAEELGLAGVALVIVLFAWLVVRAFAIGRQACLHERHFAALAAQGIGIWFGFQAL
                     INMGVNTGLLPTKGLTLPLMSYGGSGLLANFIALAILLRIDWENRQLTRGLNA"
     gene            complement(18335..19705)
                     /gene="murD"
                     /locus_tag="VIG70_08970"
     CDS             complement(18335..19705)
                     /gene="murD"
                     /locus_tag="VIG70_08970"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY8250725.1"
                     /translation="MMREPAFQPLPPPPPTPLVGRRLLVLGLGDTGLSVARWAEREGA
                     AVRVADSRRDPPRMKDFRGELRTGTFTEALLDGVDAVCVSPGLSLDEEVVRRAVLRGL
                     PVFGDIELFAWHARQPVLAVTGTNGKSTVTALAGHLLRSAGVDCEVAGNIGPPVFDSM
                     LKRLDAGKLPRAWVLELSSYQLETTWSLAPAAAAMLNLTEDHLDRYATLEDYGAAKAR
                     IFMGAGAQILNRDDPRSVAMAQTGKTVLTFGLDAPPTPKDFGVAGGKLVQGRKAILAL
                     DELPIRGSHNVANALAACALVSILDADPVAGLRTFKGLPHRLQLVATRRGVEWYDDSK
                     GTNVGATVAALRGLGRKAVLILGGDGKGQDFSPLRGPVREFASHVLLIGRDAPLIEKV
                     VAGERCVSLEAAVQRAAALAKPGEAVLLSPACASFDMFRDYRHRGEVFATAVQRLAPA
                     TKGPSS"
     gene            complement(19702..20793)
                     /gene="mraY"
                     /locus_tag="VIG70_08975"
     CDS             complement(19702..20793)
                     /gene="mraY"
                     /locus_tag="VIG70_08975"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY8250726.1"
                     /translation="MLLELAQWIAKDVRLFNVFNYITLRAVLACLTALAVSLILGPYV
                     IRKLTAYKIGQSVRDDGPRSHLTKAGTPTMGGALILVSMCLTTLLWGDLSNRLMWVVL
                     LVTLAFGTIGWIDDYRKIVHRNPKGLSPRAKFFWQAVIGLSAALYLGSLTQSMPALNQ
                     LIVPFFKHIAYPLGAVGFVVLTFFVIVGTSNAVNLTDGLDGLAIMPTVMVGSALGIFA
                     YVAGHAGFSKYLGLPYIPGAGELAVICGALAGAGLGFLWFNAYPAEVFMGDVGALALG
                     AALGTIAVIVRQEIVLFIMGGVFVAETLSVMLQVIYFKATGGKRIFRMAPLHHHYELE
                     GWKESQVVVRFWIITMMLVLFGLSTLKLR"
     gene            complement(20793..22139)
                     /gene="murF"
                     /locus_tag="VIG70_08980"
     CDS             complement(20793..22139)
                     /gene="murF"
                     /locus_tag="VIG70_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY8250727.1"
                     /translation="MMRLAEAAAALGGRASGADALFTGVSTDSRSIKEGDLFVALRGE
                     RFDGHDFLKPVAAAKAAAAMLDRKYSGEYPMPALVVDDTRRSLGDLARYWRARFAPAL
                     VAVTGSNGKTTVKEMLAAILMKHAGETAVLATRGNLNNDIGLPLTLLGLRHAHRWCAI
                     ELGMNHKGEIAYLAGIARPTVALVNNAQREHLEFMRSVEEVAAENASVYDALGEEGVA
                     VINADDPHAALFRSRAGKRRTVDFGLGGGAVTGRYRLERLSSEIRLEAPAGAADATLA
                     IPGLHNVRNALAAAACAFAVGIEVKTIAQGLSAFRPYTGRLQVKKAASGATVIDDSYN
                     ANPDSVRAAIDVLASCPAPTALVLGDMGEVGEHGAEFHREVGTYARTQGVSQLLALGD
                     ATRHAVEAFGAGAQRFDTVDELVSNLKSNTVLVKGSRFMKMERVVAALTGTPEGGH"
     gene            complement(22136..23581)
                     /locus_tag="VIG70_08985"
     CDS             complement(22136..23581)
                     /locus_tag="VIG70_08985"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY8250728.1"
                     /translation="MDPFLQLAEQGVTIERLSSDSRRCAPGVAFLAYPGEAADGRAHI
                     GDALARGAAAVLWERKDFSWNPGWSVPNLPVQDLKQRSGVLAHEFYGKPSESLWVCGV
                     TGTNGKTSCSQWIAALLGSNAMKTAVIGTLGAGFPGALAAATNTTPDALETQFLLRSF
                     LDQGGRAVAMEVSSHGLVQGRVNGVRFACALFTNLSHDHLDYHGSMAAYARAKAQLFE
                     VPGLGTAVLNLDDAFGAELARRLVGRVRTIGYSLSSQKPVGVDEFVAAERVAGAKLEI
                     HSSWGKATTTLRQIGRFNVANALGVLGCLVANGISFKEGAAMLATLPPVPGRMQAVGE
                     EPLVVIDYAHTPDALENVLHTLRPVAAERGGRLAVVFGAGGDRDPTKRPLMGAIAARL
                     ADYRLITSDNPRSEDPLAIISQIEKGMSAAHEVEADRARAIAKVISDAQSHDVILIAG
                     KGHETYQEIAGRRTHFSDEEQARAALARRTR"
     gene            complement(23594..25309)
                     /locus_tag="VIG70_08990"
     CDS             complement(23594..25309)
                     /locus_tag="VIG70_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY8250729.1"
                     /translation="MSRAAQGLVRLPVWRARVMLIMLVAGFAVLAARATYLQAVRTDF
                     LQEKGDARYSRVLEIPATRGRILDRNGEALAVSTPVKSVWAIPGDVDAGGSDRRKLAA
                     LLSMSKTDLDKRLSDTGKDFVYLKRQMAPEAAAAVQALGLKGVHEHPEYRRYYPGGEV
                     TAHVIGFTGVDDAGQEGVELAHQALLGGKPGSRRVIRDRLGRIVEDVESIRSGQDGTD
                     LALSIDSKIQNLAFGALKAAVENHRAKAGAIIVVDVRTGEILALANTPSYNPNNRARL
                     TGAQLRNRVITDLFEPGSTLKPFTVALALETGKVSASTVVHTAPGSLTLAKYTIRDVH
                     PAASQTVGEVLARSSNVGAAKIALALPREAMWDFFRRVGFGAAPELGFPGAAAGRLRH
                     YKSWRPIEQATMGYGHGISLSLAQLARAYTVFARDGELVPLTLVKTGAAVSGDKVLSA
                     EAARAVRAMLEASVQPGATGPRARIMGWRVAGKTGTAHKQENGGYAPDKYLASFVGFA
                     PVSAPRLVVAVMIDEPSAGEHYGGSVAAPVFAQVMQGALRLLGVPHDAPLELPRGGEE
                     AREST"
     gene            complement(25306..25578)
                     /gene="ftsL"
                     /locus_tag="VIG70_08995"
     CDS             complement(25306..25578)
                     /gene="ftsL"
                     /locus_tag="VIG70_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEY8250730.1"
                     /translation="MAKLNLLLLAVLVACALGLVTTQHKGRRLFSQLEREQALARELE
                     AEFGRLQLEASTWGLHSRVERLATRSLGLRPADPRRVRFVGRDAGR"
     gene            complement(25566..26528)
                     /gene="rsmH"
                     /locus_tag="VIG70_09000"
     CDS             complement(25566..26528)
                     /gene="rsmH"
                     /locus_tag="VIG70_09000"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY8250731.1"
                     /translation="MHQPVLAREAIEALALRPDGVYVDGTFGRGGHSRLILERLGAAG
                     RLIALDRDPQAAEAARAIGDPRFTFFKAKFSDLSMVPAQVHGMLFDLGVSSPQLDDPA
                     RGFSFRHDGPLDMRMDPEAGMSAAEWLARAEEQEIRGVIADYGEERFAKQIAAAIVAA
                     RRGAPIVRTRQLADLVAQAVRTREPGQDPATRTFQALRIHVNRELEEVSLMLPRAAER
                     LAPQGRLVAISFHSLEDRVVKRFMQSLARPEMPRRLPLRASEMPQPALRIVGRAQRAS
                     RQEITRNPRARSATLRVAERTAVTFTAPPISPNNRIEPGARWRN"
     gene            complement(26535..26984)
                     /gene="mraZ"
                     /locus_tag="VIG70_09005"
     CDS             complement(26535..26984)
                     /gene="mraZ"
                     /locus_tag="VIG70_09005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY8250732.1"
                     /translation="MGTAFHGAAVITLDAKGRVAVPTRHRGALLDGSKSVVLTAHPEG
                     CVLIYPETAWEPVRAKVESFPAFHPQASWWKRLLLGFEEHVTPDGSGRILLTPALRQH
                     AKLERDAMMVGQGHYFELWDAGVWAAKLGQALAGSATPPAGIEDFSL"
     gene            27207..27890
                     /locus_tag="VIG70_09010"
     CDS             27207..27890
                     /locus_tag="VIG70_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEY8250733.1"
                     /translation="MINKFVASPEAALADIPDGATVMIHGFGNAGMPTALIDALIAQG
                     ARDLTIVNNNAGNADAGLAALIGARRVRKILCSFPRQADSWHFDKLYRAGEIELELVP
                     QGTLAESIRAAGAGIGAFFTPTAYGTKLAEGKETRRIGDRDYVLEYPIHADYALIRAD
                     RADRWGNLTYRKSARNFGPLMAMAAKTTVVQVRETVDLGSIDPEHIVTPGIFVKRVVR
                     VAASLKKAA"
     gene            27890..28540
                     /locus_tag="VIG70_09015"
     CDS             27890..28540
                     /locus_tag="VIG70_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEY8250734.1"
                     /translation="MKKLDRKQMAARVARDIPEGAYVNLGIGLPTLVADFLPKGREII
                     LHSENGILGLGPKPAPGDEDEDLINAGKEPATLLTGGAYFHHADAFGMIRGRHLDFAI
                     LGAFQVSVAGDLANWHTGGPDDIPAVGGAMDLAVGAKKVLVMMEHLTKKGESKIVERC
                     SYPLTGARCVDRIYTDLAVIDVKADGLHVVESIEGLSFEELQRLTGVPLKKALIAA"
     gene            complement(28527..29489)
                     /locus_tag="VIG70_09020"
     CDS             complement(28527..29489)
                     /locus_tag="VIG70_09020"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250735.1"
                     /translation="MKNIFFALLAAWAAQFASCAHAQYPNKAVQVIISFTPASATDIV
                     GRIVTAKLAEYWGQPVVNENRAGAGGSIGSAAVAKAAPDGYTLLINSNAHSVNPAIYA
                     KLPYETTKDFTDIAPLSELPNVLVVSGESRYKTLADLVAFGKANPSRINFGHAGVGSG
                     THLNTVKLITAAGLDVTQVPFKGTPEVVAAILSGNVDCYWVPISAGLSNIKAGRLRAL
                     AVSTSKRSALLPDLATTGEAGVKGADFGLWFGVWGPAGMSAELIAKINADVRRALADP
                     GVREKLANTGNAPLDMSPQEFARFVRAEIDDYQRIVRAAGIKPQ"
     gene            complement(29486..30439)
                     /locus_tag="VIG70_09025"
     CDS             complement(29486..30439)
                     /locus_tag="VIG70_09025"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250736.1"
                     /translation="MKRLLGLFLAAFVSGVFAQGFPSKPVRIIVAFTPGSSTDIVGRA
                     VAAKLSELWGQPVVVENRAGAGGSVGSAAVLREPPDGYTLLANSSAHVANPSIYATLP
                     YDTLKDFANIAPLAGGPNVLIVAPSTGWKTLGDFITAAKAKPGALNFSSAGVGSGTHF
                     NLEKLKLMAGIDVVHVAYKGTPEAIGDTIAGRVCCYWAPINAALPHVNGGRAVALAVS
                     SAERSSLLANVPTVAEQGVPGFDYTLWVGLWGQAAMPAEIVNKINADVNRALASPDLA
                     DRLTKLGTLPMKMSSSEFAQFVRKEVDDTAKVLKAAGIKPQ"
     gene            complement(30450..31403)
                     /locus_tag="VIG70_09030"
     CDS             complement(30450..31403)
                     /locus_tag="VIG70_09030"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250737.1"
                     /translation="MRNLLFLAIALFIGSASAQTYPAKAIRIIVPFTPGSATDVMGRI
                     VGERLNAAWGQPVVVDNKPGAGGSIGIREGSRADPDGYTLVLVSSGHAVNHVLYKDLG
                     YDTLKDLSAVAMLGSLPSVLIVPPTLPVNSVKELVAMLQANPGKYNYATAGVGSGAHV
                     SLEKFNVATGVKAVHVPLKGTPPILAETMAGRVEYAMVPAVSGMGPVRDGKVKPLAVS
                     TNKRVAALPNVPTLGEAGFPGAESTFWLALLAPAKTPPEIVAKVNAEVNRALNSPEVV
                     ERLAKLGTDPMSMTPAQSDAFIAREYNELGKIMRDAGLTPQ"
     gene            complement(31400..32038)
                     /locus_tag="VIG70_09035"
     CDS             complement(31400..32038)
                     /locus_tag="VIG70_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250738.1"
                     /translation="MTPKQRISYVPVEKMDAEMQKEMDRCRREGTPRPESSAVRAHVP
                     AAFWSFANSWRDLFRNGVCEHPIKELCRLYVSRSVQCDYCGNQRSMKAASAGMIEADV
                     KDLLNFEKSTRYSGKQKAALAYAEAITWHLDTDDAFWERLHRHFSEPELVELGCFIAL
                     TMGQQSWLRLLNIEHHQVLAGTTASMAPGYEDPASLQKNKSSPDYWAKRGRA"
     gene            32074..32448
                     /locus_tag="VIG70_09040"
     CDS             32074..32448
                     /locus_tag="VIG70_09040"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY8250739.1"
                     /translation="MYQDHTALQAAGQLLLAFAFLATGVRNAGWKFRQHLDRMVAYGV
                     PQARLALLAGFVLQFVGGVLLLFDWNRTLGAGLLIAFTVLASAIFHRWWLAPDPLLAH
                     LHLSNLIVNCGVIGGLLLVAAI"
BASE COUNT         5110 a        11077 c        10934 g         5399 t
ORIGIN      
        1 cagatgcttg cgcgccagca cgaccggcgc agcgcaaaac ctgttttgcg tgaacactcc
       61 cgccaccgcc gcgccgggcg acaggcgcat gacgaggagg tccttgcgcc cgggttgctt
      121 gatgccggcc atggcgacgc ccagctcaat gccccgtacg gcgagcagcg cggcagggct
      181 cggcgcccga aggttgaccg ccatgcggct aggtcagctt gccgtggcag tgcttgtact
      241 tcttccccga gccgcagggg cagggatcgt tgcggccgac cttctgcgtg tgacgcacgc
      301 tcggctgcgc cttcttctcc tcgggcgcct cgccctcgcc cgcgccctcg gcgggctcgc
      361 cgtgctgcag ctgcacgttc tgcacgtgca gcttgtcgtc ggcctgcggc acatcctcct
      421 tggcgcggat ctcgaccgcg gtcagcgtct tgaccacttc cagcttgacc gcctcgacca
      481 tcacgccgaa cagctcgaac gcctcgcgct tgtactcctg cttcgggttc ttctgcgcgt
      541 agccgcgcag gtggatgccc tggcgcaggt ggtcgagcgc cgccagatgc tcgcgccagt
      601 gcgcgtcgag gatctgcagc accacgtagc gctcgtactg gtggaacgag ctctcggcgc
      661 cggacggcac cttcaggtgg taggcctcgt cggctttcgc cacgatgcgc tcgacgagct
      721 gctcgggctg cagctcgggc tccttttcca tccactgacg cagcggcagc tcgagctgca
      781 gctcggcctt gagcgcgttt tccaggccgg cgatgtccca ttgctcctcg acgctctcct
      841 ccggcacgta gatgcggaac aggtccccga tcaccccggc gcgcaggtcg gcgatgcgcg
      901 gagacacgtc ctgggactcg agcagctcgt tgcgcagggc gtagatcgtc ttcctctggt
      961 cgttggcaac gtcgtcgtac tcgaggagct gcttgcggac atcgaagttc cgggcttcga
     1021 ctttgcgctg ggcgctttcg atcgagcgcg tcaccatcgc gtgctcgatc gcctcgcctt
     1081 cgggcatctt cagcatcacc atgatgcggt tgatgcgctc gccggcgaag atgcgcagca
     1141 gcgggtcctc gagcgccatg tagaagcgcg agctgcccgg gtcgccctgg cgccccgagc
     1201 ggccgcgcag ctggttgtcg atgcggcgcg actcgtggcg ctcggtgccg atgatgtgca
     1261 ggccgccggc cttcaccacc tggtcgtgca gcgcctgcca ttcgccgcgc agcgtctgga
     1321 tccgttgctg tttttcattc aaatccagat ctggatttgc ttcgacgaaa tcgcactgct
     1381 tctcgacgtt gccgccgagc acgatgtcgg tgccgcggcc ggccatgttg gtggcgatgg
     1441 tgaccatctt cgggcggccc gcctgcgcca cgatctcggc ctcgcgcgcg tgctgcttcg
     1501 cattcagcac ctggtgcggg agcttgtcct tctgcagcat cccggagagc agctccgagt
     1561 tctcgatcga ggtggtgccg accagcaccg gctggccgcg gtcgtggcag tcgcggatat
     1621 cgttgatcac cgcctggtgc ttctccttgg cggtgcggta cacctggtcc tggcggtcgg
     1681 cgcggaccat cttcatgtgc gtcggcacca ccaccacctc gagaccgtag atctgctgga
     1741 actcgtaggc ctcggtgtcg gcggttccgg tcatgccggc gagcttgccg tacatgcgga
     1801 agtagttctg gaaggtgatc gaggcgaggg tctggttctc gttctggatc tgcaccttct
     1861 ccttcgcctc gaccgcctgg tgcaggccgt cggaccagcg ccgcccgacc atcagccggc
     1921 cggtgaactc gtcgacgatg atcacctcgt cgttctgcac cacgtaatgc tggtcgcggt
     1981 ggaaaagata gtgcgcgcgc agcgcggcgt agagctggtg caccaggttg atgtaggcgg
     2041 gctcgtagag gctcgcgccc tcgggcagca ggcccttgcg caccaggatc tcctcggcct
     2101 tctggtgtcc cgcctccgac agcaggatct ggtggttctt ctcgtcgacg tagaagtcgc
     2161 ccggcggctc gggctggtcg ggcttcttct cttccttctg ccgcgtcagc agcggtgcca
     2221 cctcgttcat gcgcaggtag atttcggtgc ggtcctcggc ctgcccggag atgatgagcg
     2281 gcgtgcgcgc ctcgtcgatc aggatggagt cgacctcgtc gacgatggcg tagtgcaggc
     2341 cgcgctggaa gcgctcctcg acgtgcatcg ccatgttgtc gcgcaggtag tcgaagccga
     2401 actcgttgtt ggtgccgtag gtgatgtcgg cggcgtaggc ggcgcgcttc ttctccggct
     2461 ccatatgcgg caggttgacg cccacggtca ggccgagaaa atggaagatc ttgcccatcc
     2521 actcggcgtc gcggttggcg aggtagtcgt tcaccgtgac gatgtgcacg cccttgccgg
     2581 tcagtgcgtt gaggtacgcg ggcagcgtgg cgacgagcgt cttgccctcg ccggtcttca
     2641 tctccgcgat cttgccgttg tgcaggacca tgccgccgag catctgcacg tcgaagtggc
     2701 gcatgcgcag cgtgcgcttg gaggcctcgc gcaccacggc gaaagcctcg ggcaggagct
     2761 cgtcgagggt cgcgccctcc gcgtgccgct ttttcagctc actcgtcttt tccttcagct
     2821 gcgagtcgga cagcttctcc agcgccggct cgaacgcatt gatgctgacg acggcgtgcg
     2881 agtactgctt gagcagccgc tgattgcgac tgccgaagat gcgcgccggc aggcgccaga
     2941 gggcctcgag attcgagcgt aggaattcca cgggtggcgg cgaaaaccgc cgattttact
     3001 ggaaaagctt gaccttactt gccgcgggcg acggtggtcg cgggaaccag gaacttggcg
     3061 ggattctgcg acgcgccccg gaagcgcacc tcgaagtgca ggtgcggccc ggtcgagcgc
     3121 ccggtggagc cgacttcgcc gatcttgcgt ccgcgctgca cgagatcgcc ctcgcgcacc
     3181 atgattttcg aggtgtgcgc atagcgcgtc accaggtcgt tgccgtggtc gatgtcgacc
     3241 atgtagccgt attgcggatg aaacccggca aagatcacca cgccgccggc ggcggcgacg
     3301 atgggcgcgc cgtagtcggc gatgaagtcg atgccttcgt gcatcgccca ctgcccgttg
     3361 aaggggtcga tgcgccggcc gaagcccgag gggttgtagg acgccttcac cggcatcatc
     3421 gtcgggatca ttttcttctt caccgcctgc tcgaagagct gcgcctcgag cacgccgagc
     3481 atgtcgttgc ggctttccac ctggcgcgcg agcaccagca gcttctcgct gaaatcgccg
     3541 agcgaaaggt tctgcggcgg catctgggtc ggggcggcgc cgcccatgcc gggcgcctcg
     3601 gcggcgcgga attcctgagg cttgatgccg gcgagcgcgg acaggcgctc gcccagcgca
     3661 tccatgcgcg tgagctgcgc ctgcatctcg ccgagcttca tggccatcga gttgaggttc
     3721 tgctggacga acacgcgctc gcgttcggct tcggcttcct gggcggcggc gacgagctgc
     3781 tgcagcgccg gcaggcggac ttcggcggca tagcgcagcg tgagccagta gagcgtggcg
     3841 gtggcgacga acagcagtag cagcgcagca aacgcagtgc cgacgaggtg ccgcagcgac
     3901 atgtggactg atctcgccct cgccagccgg tccgagatca ggataatctg cacaacgact
     3961 tccctccctg cttatggtgc gcccgcccaa gctcggccga attctcgcgg ccgaggccac
     4021 tctgcagccg gtcctcgcga aggcgcacga gttgcgtgcg ctcgccgggc tgcttgacgg
     4081 gtttctgccg ccggacttgg cgcgccagac ccgagttgtc aattgccggg acgctgaact
     4141 ggtgctggtg gcggcctctc cggccgccgc ggcgaagctc cggctgctcg ccccgtccct
     4201 ggcaaatttc tttttaaaac agcgcttgca ggttaattca gtttccatac gggtgcaacc
     4261 aaacgcatca cctcgcacgg gtgctgcacc gcaaaaaagt gtttactttt ccacacctgc
     4321 gctcgatagg ctgagtagcc tttacaaaac gatgcgcaac tccccggcgc gcgaggcatt
     4381 acgcgccctg cttgtgcacc atggggctac tccaggaggg cctccccggc aaacaggacc
     4441 aggatcgcca acagcgcgaa aacggcgtac ttgaagacca cccaggtgat ccggcgccag
     4501 cgcgggtcac cggtgagcag ccaggcgagc atgctgacgc cgagcacgat cgcgaccagc
     4561 acgaccgcga cccgcaggac tgcgaacacc tagcctagct gcgtagacag acgggccacg
     4621 cccgccggcg cctcttcggc gcgctcgaac accacggtct cgagcgcccc ggcatcggcg
     4681 agcgcggcgc gcaccagctt gttgttgagg ccgtggccgg acttgtgcgc ggtgaatgcg
     4741 ccgagcagcg gccgcccggc aaggtacagg tcgccgatcg cgtcgagcag cttgtggcgg
     4801 atgaactcgt cggcgaagcg caggccgtcg ctgttcagca cgccctgctc gtcgagcacg
     4861 acggcgttct cgaggccgcc gccgagcgcg aacccgctgt ccttcagatc ctccacctcg
     4921 tgcatgaagc cgaaggtgcg cgcgcgcgcg atctccttga ggtaggagga ctcggcgaaa
     4981 tccaccgtca ccgactgggt gctgcgctcg atcatcggat ggcggaactc gatcgagaac
     5041 gagagcttga agccttggta aggctcgagc cgcgcccact tgtcgccgtc gcgcacttcg
     5101 acggatttct tgacccgcag gaaccgcttc ggggctgcct gctcctcgag gcccgcctgc
     5161 tgcaggagca gcacgaacgg cgaagcgctg ccgtccatga tcggcagctc gggcgcgtcg
     5221 aggtcgacga aaacattgtc cacgcccagc ccggagagcg ccgacatcag gtgctcgacg
     5281 gtgtagacct tcacgccgtc cttgaccagc gtggaagcga ggcgggcttc gccgaccagc
     5341 tcggcgcgcg ccgggatgtc caccggcgag ggcaggtcga tgcgccggaa cacgaccccg
     5401 gtgtcgggcg gcgcaggccg cagcactatg cggactttct ggccggtatg aaggccgacg
     5461 ccggaggcgc ggatcaggga cttcagggtg cgctggcgca gcattaggga gagtctagca
     5521 agcctcaggt atcgccgccg ccgcgctcct tgagcaacgc gcgataacgc tgctcgctct
     5581 gcttggcgcg ctcttcggcc agcaggcgct cgctcagatc ctcgatcacg ccgaggcgcg
     5641 cgcggcgccc ggcgaactcg aggttgaacg aggtcacccg caccgggatc acctcgccgc
     5701 tcttcttctt gtgccgccaa atgcgctggc tgtagttgtt gctccagtcg gcgaaggcgc
     5761 ggcgcaggtc gtcgacgtcc tcctcggggc ggatctggtc ggcggtgagc gtgagaaact
     5821 cttccttgga atagccgtac agccgcatcg cggcgccgtt caccgcgagg aacgcgaacg
     5881 tgttggcgtc gaccacccac atcggctgcg gatgcacgtc gaacagggcg cggtagcgcc
     5941 gctcgctgtc ctcgagcgcc ttctggtcgc gggcgcgctc ggtgatgtcc ttgcccacgc
     6001 cgtggtagcc gatgaacttg cctttctcgt cgaacagcgg ctcgccgctc accgagaggt
     6061 agcgcagctc gccggacttg ttgtagcgca ggaacacgaa atcggtgaac gactcgtggc
     6121 gctcgagctt ggcgcggtgc tgctcccaga ccttctcggg cagcgcgccg aggccgggga
     6181 tctcccagcg cgtcttgccg atgacgacat cgctgtcgag ctccggctcg agtcccgaca
     6241 cctcgatgaa gcggaactgg tcgtcctgca tccagtacca gtccgagctc atctcgacca
     6301 tgcggcggaa gcgctgcttg ctcttgcgca ccgagcgctt gagggcggag atgtcgctcg
     6361 ccaccagcac ggtgcattcc ggcgcgccgg actcgtcccg caccaccagg ccgttcatct
     6421 gcacccagat ggtggagccg tccttgtgga cgtagcgccg ctcggcgacc ttgggcttca
     6481 tccttccgga gatcacctgg gcgcgcgcca cggcggcggc ttcgagatcg tctgcgtgga
     6541 tcacgtcgag atgcgtcttc tgctgcagct cgtgctcggt gtagccgagc aggcggcaca
     6601 tcgccgggtt cgcgcgcaga aagcgtccgc cgatcgaaaa cagggcgatc gcgaccgccg
     6661 cctgctcgaa catcgcgcca aacagcgctt cacccgaagg agccgattcc gccatggaag
     6721 gaatcatgac acagccgagg atccggggtc acccccggca aggtggggta accccggcct
     6781 gacgcttggc tctacttgcc gccgaacatt ttcggatcgg cgggtctcag ctccccgaac
     6841 aactgacgca tttggacgag cccatcgatg ttcctcaacg tcgctgccga gcccgcttcc
     6901 attagcttcc tatagtgctc cggcgagttc acgacggtgg gatacccttg ctgaagttga
     6961 ggcctcggct tgctccctcc ccggcggctg gtgtcctgtg acgccgcctg gcgcgcgtcc
     7021 agccgggcgt ccagcgcggc gatgtcgcgc tgcagctgcg cacgggagcg ctcctcgtac
     7081 gccttgcgat cctgctcgac gcgcttcatc gtcgccgcca tgttcgccga cgcgaggttg
     7141 gcggcgctca aggaggaata gtccgtggcc ggggtgtcga gccagggagc gagcgggaac
     7201 tggacacgcg aggcgccgcg cgcggccggc gcagccagcg ccaccggcac gtcgatctcc
     7261 tctccgcggc gcaggacatt gagaacggcc tgcgtaccgc ggcgggcggc gatccgctcg
     7321 gtgagcgcgc gagcgtcggg gacgcgctcg ccgtccatgc cggccaccac gtctccctgc
     7381 cgcagcccgg cccgcgctgc cggcgtgccg ggggtcacgc tgccgaccat gacgaggttc
     7441 atctcgcgtc cgaccagctt caggacctcc tccggcagat cccggagggt cgcgccgaga
     7501 ctgaaggcac ccggatccag cctgccccag taggtgacga ggtagtagtg caggttgctc
     7561 tctcccggcc ggggcgtttc catcaacacg cgggcggcgc cgagctccgc gccccacttg
     7621 gtcgaatagt cgggcgcgag gctcgtcagc aaggggctga cgacctgcga gtagccgagc
     7681 agcgcgtagc cctcgttcca catcgcgggc gatttttcca ctaggtcgcc ggtctgttcg
     7741 aaggccggcg cgccactgtg gcgcagcaat agcgggtcgt tggcgttggc gatggcctgg
     7801 tagctgctcc cgaagtacat ggtctgcatc gacgtgcagg ccgacgtcag caggaccgcc
     7861 aggatcacga gtagggactt gcccggactt gccatggcac ctgccttgta gacgcggccg
     7921 ttcgccagct cgccgtaaga ctcggccgcg aacgcgccgc gggcgaagag ctcctccgcc
     7981 actctccggt tctgcgctgc gctgattctt ggatccagtt tgtattggtc cgcttacggt
     8041 cccttgcgtt gacggtcctt tgcattgacc gacccttgca ggtccataaa gaggtttgtc
     8101 attgcatcgg cgcgccttcg gcagaatggt tttcgcatgt tcgtcccaac aaacgaaaaa
     8161 ggaatgcagt catgcgaatg aacgtcctgc ccctcagcgc cgcgcttctt ctggcctgcg
     8221 ccgcgccagc cttcgcggcg cccggcgctc tcgacgcgaa cgcgtcgctg ccggcggtga
     8281 agcgcatcgc ggccgagatc gccgcggatc tcgcgcgcgt ctgcccgctg gcgcagcccg
     8341 gcgaccaggc ggcgttcgat cgttgccgcc aggcgctgtt cgacggctcg ccgctcaagc
     8401 gcaacatgca ctcgatcgtg atctggggcc gggtgaagcc cggcgctgcg ctcaaggaga
     8461 cacatctcac ccagttcgcg cccgacgtgc tcaccgggat gtacatcccg cttttcatgt
     8521 tcaacggcaa gcacacgatc gcgttcgacg agcgcgagaa cctgttcgtc gtgcgcctgg
     8581 aagccgggtt ccgcaaccgc ctgcagcccg gccagttccc gtatccgttc tggcattcag
     8641 acgcgaagtg gagcacctac caggctgcca acaacctgct gctgtggatc gatccgaaga
     8701 gcgtcactat ccggtacggc cagttcaccg accacgggac cacggcgccg atcagccttt
     8761 ccgctccggt gaagcacgag ttcgacggca agtggatgtg gaccgacgcc gccggccggg
     8821 tgcagccgca ggcgacgctg ttcgacggcc tgttcagccc cgacaatccc tacctgcaca
     8881 agctcgacgg cgcctatcgc aagctggcga tcggactgcg cgaaggccag tgcagctcct
     8941 gccatgaccc cgacaacaag cacgacatga agcgcctggt gctgctgcag acgccggcgc
     9001 atgccgccgg cgaaatcaag cgcgtcatca aggccctgcg gcgcgacagc atgccgctcg
     9061 acgacgccgg catcgagcag ccgctcgaag gcccggtgaa ggaggcgctg ctcaagcacg
     9121 ccggcgcctt cgacgcgctg gtcgacgccg ccaaggaatg ggagcgcgtc cacgccgcgc
     9181 gcaacgacga ggcgggcaag cgcactgcgg cagtccgcta gctcaccgcc acgttttttc
     9241 cgccccgcgg cacacaagga gagcctgctc ggcgtcgctg acattcctcg tcaggcaatg
     9301 gcgccgcgcg ttaccgtaga ctgaaggcgc cttttaaccg aggggaggaa gcgatgaaga
     9361 gcgccaaatg gatggtgggg gcggggctgg ctgcgctcgc agcggtaacg gcgtacgcgc
     9421 agttgccagg cattacgcgc acgccggtgc aggagcagga tctaggcagc ctgccgggcc
     9481 gccaggcggt ggtggcgcgg gtcgagttcg ctcctggcgc gttggttccc aagcacacgc
     9541 acgcgggcga ggagatcggc tacgtggtcg agggcacgat cgagctcatc gtcgaaggcc
     9601 agccgccgag aatcgtgaag gcgggcgagg ggttcctggt gcccgccggc atcgtgcacg
     9661 agggcaggaa caccggcagc gacaaggcgg tggtgctcgc cacctacgtc cacgagaagg
     9721 gcaagccggt cgcgacgatg gtcaagtagc cccgaagcgc aggaaatagc tgccgccccc
     9781 ggcaaggttg ggaggcggca gctttgctac gagcgggcgc tagtccgcct gcttcctgag
     9841 aaacgccgga atgtcgtact tatcgacgcc gctctgctgc agcgcctcca ccgtcgaggc
     9901 gcgattgcgc cggatgaccg ccgggacctg gtcgagctgc tggtagtcga ctgggccgtg
     9961 cgcgttgtcg gtcccggtct tctgcgcggc aacgttgttg agcacgagct gcggtttcgg
    10021 ctgccgcgcc gcctgcgcca ggccgaggcc ggtcgcgacg acggtgacgc gcaggtcctc
    10081 cgccagcgcg tcgtcgtaga cgccgccgta gatgatggtg gcgtcttccg cggcgaaggt
    10141 gcggatgatg ttcatcacct ccttcgtctc gcgcagcttg aggctcggcc gcgaagccgt
    10201 gatgttgacc agcacgccgc gggcgccggc gaggttcacg ccctcgagca gcgggcaggc
    10261 gatcgcctgc tcggcggcga tgcgcgcgcg gtcgatgccg ctcgccgagg ccgaccccat
    10321 catcgccatg ccctgctccg acatcaccgt cttcacgtcc tggaagtcca cgttgaccag
    10381 gcccggcagg ttgatgatct cggcgatgcc ggcgacggcg ttgttcagca cgtcgtcggc
    10441 ggcgcggaac gcatcctcct gcgtcacgtc ctcgcccatc acctcttcga gcttgtcgtt
    10501 gaggatcacg atcaacgagt cgacatgcgg ctgcagcgct tcgatgccgg cttcggcgat
    10561 cttcatccgc ttggaaccct cgaaggtgaa cggcttggtg accaccgcca ccgtgaggat
    10621 gcccaggccg cgcgcgacct cggcgatcac cggcgccgcg ccggtcccgg tgccgccgcc
    10681 catgcccgcg gtgatgaaca ccatgtgcga gccggcgacc gcttcttcga tgcgctcgcg
    10741 ctcggcgagc gccaccgtgc gcgcctcttc gggctttgcg cccgcgccca ggccgctcga
    10801 gccgagctgc agctggttgc gcgccttgtt gcgcgcgagc acctgcgcgt cggtgttcac
    10861 ggcgatgaac tccacgcctt gcacgccggt ctcgatcatg tggttgatgg cgttgccgcc
    10921 agccccaccc accccgatga ccttgatcac cggtccggtg gcttgactat cgactatctc
    10981 gaacatgtcg tcgtctcctt gcctgttgtt gagtacttag aaattgcgtt gaaaccactc
    11041 ccgcatgcgc cccagaacgg ccttgaccga gccgctctgg cgcgaaagct tgccctgctc
    11101 catctgtgcc atgccctcgt acagcagccc cacgccggtg gcgtggcgcg gcgtgcgcag
    11161 cacttccgcc agcgcgccgg cgtagcgcgg gatgccgacg cgcaccggca tgtggaagat
    11221 ttcctcgccg agctcgacca tgccctgcat caccgccgag ccgccggtga gcaccacgcc
    11281 gctcgacagg agctcctcga acccggactc gcgcagcacc tgctgcacca gcgaatagag
    11341 ctcctcgacg cgcggctcga tcacctcggc gagcgtctgg cgcgacatcg agcgcggcgc
    11401 gcgctcgccg atgcccggca cctcgatgct ctcgcccggg tcggcgaggt tgcgcagcgc
    11461 gacgccgtgg cggatcttga tgttctcggc gtcggcggtc ggcgtgcgca gcgccatcgc
    11521 gatgtcgttg gtgatctggt cgcccgcgat cgggatcacc gcggtatggc ggatcgcgcc
    11581 gtgggtgaac accgcgatgt cggtggtgcc gccgccgatg tcgagcaggc acacgccgag
    11641 gtccttctcg tcctcggaca gcaccgcgcg cgaggacgcg agcggctgca ggatcaggtc
    11701 gctcacctcc aggccgcagc ggcgcacgca cttcacgatg ttctgcgccg ccgacaccgc
    11761 gccggtgacg atgtgcacct tcacttcgag gcgcacgccc gacatgccga tcggctcgcg
    11821 cacgtcctcc tggccgtcga tgatgaattc ctggcgcagc acgtgcagga tctgctgatc
    11881 ggtcgggatg ttcaccgcgc gcgcggtgtc gagcgcgcgg ttgacgtcga gcgcgctcac
    11941 ctcgcggtcc ttcaccgcca ccatgccggt cgagttgaag ctgcggatat ggttgccggc
    12001 gatgccgacg aaggccgagg cgatcttgca gtccgccatc aattccgcct cctcgagcgc
    12061 gcgctggatc gcggccacgg tggcctcgat gttcaccacc acgcccttct tcaggccgcg
    12121 cgactcgtgg ctgcccatgc cgagcatggc gagcgcgccg tcggcgccga gctcgccgac
    12181 gaggcatgcc actttggaag ttccgatgtc gagggccacg atgaggttct tgttttcttt
    12241 gggcatctct ctcagccgtt ccagtccggc acgcgcagcg cgaagccgtt cgggtagcgc
    12301 aggtcgacgt actcgtgctt cttcccggcg cgggcgaggg tcgccggatg ggcggcgacg
    12361 aatttcctca ggcgctcctc ggcgaggtcg gcgtcgcgcc cgagcatcag gtgcaggccg
    12421 ttgcccaggc gcagctgcca ggcatggcgc ggcgtgagca ccacgcgctc gactccggtg
    12481 ccaagcggcg ccacgatctc ggcgaagcgc cggtaacggc gcgtcacctc ggcggaggtt
    12541 ccaccgggag cgatgaaggc aggcaggggc tccttggtct ttgcaacaaa cctctccccg
    12601 tgagtgttca cgagggcgtc gtcgccccag cgcgccagcg cgatgtgctc ctcgacccaa
    12661 acctccaaac ggttcggcca cacgcggcgc actgcgacgc ggcgtgccca gggcaagcgc
    12721 tcgaccccag cgcgcgcgtt cgcgaggtcg acggcgaaga aatttcctga ggcgcgcggc
    12781 agcgccgctt cgatctcggc tgcggtggtg tgcttcaggt tgcccttgag cacgacctcg
    12841 ctgaccggga agcgctcggg agcgagcagc cagtagagcg cgccggcgca agcggacagc
    12901 atgagcaggc ctgcaaggat tccgaagaaa aggctcaccc tagccgacat gcgcaagctc
    12961 caggatcttc acgcacaggt cctcgtagga catgccgacg gcgcgcgccg ccatcggcac
    13021 gagcgagtga tcggtcatcc cgggcgaggt gttcacttcc agcaggaacg ggcgtccgcg
    13081 cgtgtcgagc atcaggtcga cgcgacccca gccgcggcag ccgagcgcgc gaaacgcctt
    13141 gaggcatagc gcctggatct ccttttcttt cttgtcgctc agcccgcaag gaatgagata
    13201 gcgcgtgtcg ttcgcgatgt acttggcctc gtagtcgtag aactcgcgcg gcgtctcgat
    13261 gcggatgatg ggcagcacct ggtcggccac aatcgccacc gtgagctccg gaccgtcgat
    13321 gaacttctcg gcgatgacga tggggtcgta attgacggcg agcgcgtatg cctcctccag
    13381 gcctgatgcc ttttttacct tcgtcattcc gaccgacgag ccttcgctcg ccggcttgac
    13441 gaaaagcggc gtgccgagtt tcttcgcgac cgctttcaga ttgctgtctc cggcgagcac
    13501 ttcatagggc gcggtcggca gcctctccgc cgcccacagg cgcttggtgc gcaccttgtc
    13561 catcgcaagc gccgaggcga gcacgccgct gccggtatag gggatgccga gccattcgag
    13621 gacgccctgc accgtgccgt cctcgccgaa gcgcccgtgg agcgcgatga aaacgcgcgc
    13681 aaagcgctcc ttgaccagat cctgaacgtc ccggtccttg gggtcgaacg ggtgcgcgtc
    13741 gacacccttc ttgcgcagcg ccgcgagcac catgcctccg ctcttgagcg agacctcgcg
    13801 ctcggccgac ttgcccccca gcagcaccgc aaccttgccg aaatgggagg tcatgatgag
    13861 gcttcttccc ccacgatgcg gacctccgtg gcgaggtaga cgcccttcat ctggtagacc
    13921 atgcgctgga cgtggcggat gagccactcg atatcgctcg ccgccgccgc gcccttcgga
    13981 ttgacgatga agttggcgtg cttctccgat acgcgcgccc cgccgcgctc gaagcccttc
    14041 aggccgcacg cttcgatcag atgcgcggca tggtgcttcg gcggattgcg gaacacgctg
    14101 ccggcgttcg gcagcgacag cggctgcgtg gcgatgcgtc gggcgagcag ctccttcatc
    14161 acggcgcggg aggtcgcgcc gtcaccgggc ttgaggcgga accaggcggc ggcaaaccac
    14221 tcttcgcccg ggctcttcgg ggcgcagtgg cggtagccga tgtcgtactc gtcgcgcccg
    14281 cgcacgatca tcttgccgcg gcggttgatg gtcgccacgt gctcgaccac gtcccaggtc
    14341 tcgccgccgt agcaccccgc gttcatcgcg agcgcgccgc cgacggtgcc gggaacgccg
    14401 gcgaggaatt ccgcgccttc gaggtcgtgc atcgcggcga agcgcgccac cttcgggctg
    14461 gcgacgccgg cttcggcata gatcttgccg tcgcgcagct cggggcggtg cgaagcggcg
    14521 tgcatcagga tcgccgtgcc gcgccaaccg ccgtcgcgca ccagcaggtt cgagcccagg
    14581 ccgacgaaaa ggagcggctc gtcttccgga agctggcgca ggaatgcgcc caggtcgtgc
    14641 aggtccgccg gcgcgtagca ccgatcggcg cggccaccgg tgcgccagct cacgtggcgg
    14701 cccatcggct cgtcgcgccg cagttcgccg cgcagcttct ggaagcggat tggctcgctc
    14761 atattcatat caactgtgaa gggaccgcgc cgatcgaccc cgcccccatc gtaatgacga
    14821 cgtccccctc ctgcgccgtg cgccggatgg tgtcgggcat ggcggcgatg tcctcgacga
    14881 agagcggctc caccttgccc gcaagccgca gcgcgcgcgc gagcgagcgg ccatccgccg
    14941 cgacgatcgg cgcctcgccc gccgcgtaga cctccgcgag cagcagcacg tcgacgccgg
    15001 agagcacgcg cacgaagtcc tcgaacaggt cgcgggtgcg cgtgtaccga tgcggctgga
    15061 acgcgagcac gatgcgccgg ccgggaaacg cgccgcgcgc cgcctcgagc gtggctgcca
    15121 tctccgcggg gtggtggccg tagtcgtcga tcagcgtgaa cttgccgtcg atctcgccgt
    15181 agttctggaa gcggcggccg acgccgcgga attccttcag cgccttctga atggacgcgt
    15241 ccttgacgcc gatctcggtg ccgaccgcga tcgcggcgag cgcgttcagg acgttgtggc
    15301 gccccggcag gttcagcacc acgtcgaggg gcgccgcgcc tttccgcagc gcgcgaaaac
    15361 gcatgcggcc gccgtcatgc tcgatcgcct cggcgcgcac cgatgcgtcc tcgccggtgc
    15421 cgtaggtgag gacgggcttt gtcacctcgg gcaggatctc gcgcacgttg gcatcgtcca
    15481 cgcacagcac ggcagcgccg tagaagggca ggttctgcag gaattgcgcg aacgcctgct
    15541 tcaggcggcc gaagtcctgc tggtacgtgt ccatgtggtc cgcatcgatg ttcgtcacca
    15601 ccgcgatcac cggctgcaga tgcaggaacg aggcgtcgga ttcgtcggcc tcgaccacga
    15661 tgaagtcccc ggcgccgagg cgggcgttgg agccggcggc attcagccgg ccgccgatga
    15721 cgaacgtcgg gtcgagcccg gcctcggcga gcacgctcgc gacgaggctt gtcgtagtgg
    15781 tcttgccgtg cgtgccggcg atcgcgacgc cctgcttcag gcgcatcaat tccgcgagca
    15841 tcagcgcgcg cggcaccacc gggatgcggc gctcgcgcgc ggcgatcacc tcggggttgt
    15901 cggcctgcac cgccgaagac acgaccaccg cgtccgcccc gttgatgtgc tggggcttgt
    15961 ggttcaggga gatcttggcg ccaagcgagg cgagacgttt cgttgccgtg ttctcggcaa
    16021 ggtcggagcc gctcacctgg tagccgaggt tggcaagcac ctccgcgatg cccgacatgc
    16081 ccgagccgcc gataccgacg aaatggatgt ggcggacctt gtgcttcact tcgcaatctc
    16141 catacagcgc tgcgcaacga cgcgggcagc atcgggtttt ccgagcgcgc gcgccttccg
    16201 cgccatgtcg agcagcctcg cgcgatcgag ggagcggatg agcgccgcca gcttttccgg
    16261 cgacaggtcg cgctgctgga tcaggatcgc cgctccgcgg tcggacagaa atttcgcgtt
    16321 cgcggtctgg tggtcgtcga ccgcgtgcgg gaacggcacc aggatgctcg ccatgccgcc
    16381 cgccgacagc tcggcgatgg tcaccgcgcc ggcgcggcag atgacgaggt cggcctcggc
    16441 gtagcggcgc gccatgtcgt cgatgaacgc gaccagttcc ccctccactc gcgcggcgag
    16501 gtaattggct tgcaagctct ccagatgctt ttcgccggcc tggtgcacga cgcggggctt
    16561 ttgcgggaga agcgaaatgg ctttgggcag cgcctcgttc agtgcctgcg caccgaggct
    16621 tccgccgacg acgaggatac gaatttgccc gtgcctgctc tcgaaacgct tttccggagg
    16681 ggcaatcgcg gcaatctcgg cccgcaccgg gttgccggtc cattcggcgc ccttcagcgc
    16741 gtccgggaaa gcgaccatga ccttgtcgct cacctgcgcg agcactcggt tggcaaggcc
    16801 ggcgatcgcg ttctgctcgt ggagcgcaag cggccggttg agaagcgagg ccatcatgcc
    16861 gccgggaaac gcgacgtagc cgccgaggcc gagcaccacc gagggcctga cggctcggat
    16921 gtgccgcgcg ctctcccaga aggagtaaag caggttcgcg ggcagcagca gcttctgcag
    16981 cagccccttg ccgcgcgccg ccttggcgcg gatccacgcg gtgcgatagc cgtgccgcgg
    17041 cacgagtcgc tcctccatgc cgccccgcgc gcccatccag acgacgtccc atccggccgc
    17101 gcgcatctgc tcggcgaccg cgaggccggg gaagatgtgg ccgccggttc caccggccat
    17161 gatgaggatg cttttcatgc attcagcccg cgcgtgagct ggcggttttc ccagtcgatc
    17221 cggagcagga tcgcgagcgc gatgaaattg gcgagcaggc ccgacccgcc gtagctcatg
    17281 agcggcagcg tcaggccttt ggtcggaagc aggcccgtgt tcacgcccat gttgatgagc
    17341 gcctggaagc cgaaccagat gccgatgcct tgcgcggcga gcgcggcgaa atggcgctcg
    17401 tgcaggcagg cctgccgccc gatggcgaag gcgcgcacca ccaaccaggc gaaaagcacg
    17461 atgaccagcg cgacgccggc gagcccgagc tcctcggcga tcaccgcgag caggaagtcg
    17521 gtgtgcgcct ccggcaggta gtgcagcttc tccacgctcg cgccgagccc tacgccgagc
    17581 cactcgcccc tgccgaatgc gatcagcgcg tgcgacagct ggtagccctt gccgaaaggg
    17641 tcggaccacg ggtccatgaa gccgaagatg cgctgcatgc ggtagggcga gaacaccacg
    17701 agcagcgcga agcccgccgc cagcatgccc agcagcgcgg cgaaatgccg gccgttcatg
    17761 ccgccgagaa acaggatcga gaacgcgatg acggcgatca ccaccagcgc gccgaagtcc
    17821 ggctcgcgca gcagcagcca cgacacgagc agcatcaccg cgagcatcgg cagcagcccg
    17881 cgccgaaagc ttttcagcac cgcgtgcttg cgcgcggtgt agtccgccgc atagagcacc
    17941 acggcgagct tgaccgcctc ggagggctgg aagccgaagc ccggcaggtc gagccagcgc
    18001 cgcgcgccgt tcacttcgcg cccgaacccc ggcacgagca ccagcgcgag accggcaacg
    18061 ccggcgacga acagccacgc cgccgccttc tgccagaggc gcgccgggac gaggaacacg
    18121 gtgatcgccg ccgcgagcgc cagcccgaga tacgcagcgt ggcgcatcag gaaccacgtg
    18181 gcgttgttcg cggtgtagcg gctcgcttcc gcggtcgcga tcgacgccga gtacaccatg
    18241 accagcccga tcgcggcgag gagcagcgcg gcccaggtcg ccgagcggtc gtattcggcg
    18301 ctcggcgcgt tgcgcgcgaa gacgtaggtc tggctcacga agaaggccct ttggtagccg
    18361 gcgcgagcct ctgcaccgcc gtcgcgaaca cctcgccgcg gtggcgatag tcgcggaaca
    18421 tgtcgaagct cgcgcacgcc ggggaaagca ggaccgcttc accgggtttc gccagcgctg
    18481 ccgctcgctg aacggccgct tcgagagaga cgcagcgttc tcccgccacc accttctcga
    18541 tcagcggcgc gtcgcggccg atcagcagga catgcgacgc gaattcccgc accggcccgc
    18601 gcaacggcga aaaatcctgc cccttgccgt cgccgccgag gatcagtacg gccttgcggc
    18661 caagcccgcg cagggcggcc acggtcgcgc ccacgttcgt gcccttggaa tcgtcgtacc
    18721 actcgacgcc gcgccgcgtc gcgacgagct gcaggcggtg aggcaagcct ttgaaggtcc
    18781 tcaggccggc gaccgggtct gcatcgagaa tcgacaccag cgcgcacgcg gccagggcgt
    18841 tggcgacgtt gtgcgagccg cggatcggca gctcgtcgag cgccaggatc gcctttcttc
    18901 cctgtaccag cttgccgccg gccacgccga agtccttcgg cgtcggcggc gcgtcgagcc
    18961 cgaaggtgag cacggtcttg ccggtctgcg ccatcgccac ggaccgcgga tcgtcgcggt
    19021 tcaggatttg cgcgccggcg cccatgaaga tgcgcgcctt ggcggcgccg tagtcctcga
    19081 gggtggcgta gcgatcgaga tggtcttccg tcaggttgag catcgccgcc gccgcgggcg
    19141 cgagcgacca ggtggtctcg agctggtagc tcgacagctc gagcacccag gcgcggggca
    19201 gctttcctgc atcaagacgt ttcagcatcg aatcgaaaac cggggggccg atgttcccgg
    19261 ccacttcgca gtccacgccc gcgctgcgca gcaggtggcc ggcgagcgcc gtcacggtgc
    19321 tcttgccgtt cgtgccggtc accgcgagca cgggttgccg cgcatgccag gcaaagagct
    19381 cgatgtcgcc gaataccgga aggcctctca ggaccgcccg acggacgact tcctcgtcaa
    19441 gcgagaggcc cgggctcacg cagaccgcgt caacgccgtc gagcaaggct tcggtgaacg
    19501 tcccggtgcg cagctcgccc cggaaatctt tcatccgcgg aggatcgcgg cggctgtcgg
    19561 cgacacgcac tgcggcccct tcgcgctcgg cccagcgcgc gaccgaaagg ccggtgtcgc
    19621 ccagaccgag caccagcagg cgccgcccca ccagaggcgt cggcggcggc ggcggcaggg
    19681 gctgaaaggc cggttcgcgc atcatcggag cttgagcgtc gacaggccga acagcacgag
    19741 catcatggtg atgatccaga agcgcaccac cacctggctc tccttccagc cttcgagctc
    19801 gtaatggtgg tgcagcggcg ccatgcggaa aatgcgcttg ccgccggtgg ccttgaagta
    19861 gattacctgc agcatcaccg acagcgtctc ggcgacgaat acgccgccca tgatgaacag
    19921 cacgatctcc tggcgcacga tcacggcgat ggtgccgagc gcggcgccga gcgcgagcgc
    19981 gccgacgtcg cccatgaaga cctcggcggg gtaggcgttg aaccagagaa agccgaggcc
    20041 cgcacccgcg agcgcgccgc agatcaccgc cagctcgccg gcgcccggga tgtacggcag
    20101 cccgagatac ttggagaacc cggcgtggcc ggcgacgtag gcgaagatgc cgagcgcgct
    20161 gccgaccatc accgtcggca tgatcgcgag gccgtcgagc ccgtcggtga gattcaccgc
    20221 attcgaagtg ccgacgatca cgaagaacgt cagcacgacg aagccgaccg cgccgagcgg
    20281 atacgcgatg tgcttgaaga acggcacgat cagctggttc agcgccggca tggattgcgt
    20341 gagcgatccg agatacagcg cagccgacag gccgatcacc gcctgccaga agaacttggc
    20401 cctgggcgac aatcccttcg gattgcggtg cacgatcttg cggtagtcgt cgatccagcc
    20461 gatggtgccg aaggcgagcg tgaccaggag caccacccac atcaggcggt tcgacaagtc
    20521 gccccacagc agcgtggtca ggcacatcga caccaggatc agcgcgccgc ccatggtggg
    20581 cgtgccggcc ttggtgaggt gcgagcgcgg cccatcgtcg cgcaccgact ggccgatctt
    20641 gtacgccgtg agcttgcgga ttacgtaagg gccgaggatc agcgacacgg ccagggcggt
    20701 caggcaggcg aggaccgcgc gcagcgtgat gtagttgaag acgttgaaga ggcgcacgtc
    20761 tttcgcgatc cattgcgcca gctcgagcag catcagtgcc ctccctcagg ggtgccggtt
    20821 agcgccgcca caacgcgctc catcttcatg aagcgcgagc ctttgaccag cacggtattg
    20881 ctcttcaggt tggagacgag ctcgtccacc gtgtcaaagc gctgcgcgcc ggcgccaaaa
    20941 gcctcgacgg catggcgcgt cgcgtcgccc agtgcgagca gctgcgaaac gccctgggtc
    21001 cgcgcatagg tgcccacttc gcggtggaac tcggcgccgt gctcgccgac ctcgcccatg
    21061 tcgccgagca ccagcgcggt gggcgccgga cacgacgcga gcacgtcgat cgccgcgcgc
    21121 accgagtcgg ggttcgcgtt gtagctgtcg tcgatgaccg tggcgccgct cgccgccttt
    21181 ttcacctgga gacggccggt gtacggccgg aacgcgctca ggccctgggc aatcgttttc
    21241 acttcgatgc ctacggcgaa cgcgcacgcg gcggccgcga gcgcgttgcg cacgttgtgc
    21301 aagcccggga tcgcgagcgt cgcgtcggcc gcgccggccg gagcctcgag gcgaatctcg
    21361 ctcgacaggc gctcgagccg gtagcgcccg gtgaccgcgc cgccgccgag gccgaagtcg
    21421 acggtcctgc gcttgcccgc gcggctacga aaaagggccg cgtgcggatc gtcggcgttg
    21481 atcaccgcca cgccttcttc gcccagggcg tcgtagaccg aggcgttctc ggccgcgact
    21541 tcctcgaccg agcgcatgaa ttccaggtgc tcgcgctggg cgttgttgac cagcgctacc
    21601 gtcgggcgcg cgatgccggc aaggtaggca atctcgccct tgtggttcat gccgagctcg
    21661 atcgcgcacc agcggtgcgc atggcgcagc ccgagcagcg tgagcggcag cccgatgtcg
    21721 ttattgaggt tgccccgcgt ggcaagcacg gccgtttcgc ccgcgtgctt catgagaatg
    21781 gccgcgagca tctccttgac ggtggtcttg ccgttcgagc ccgtcaccgc gacgagcgcc
    21841 ggtgcgaagc gcgcgcgcca gtagcgcgcg agatcgccga gcgagcggcg ggtgtcgtcg
    21901 accaccagcg ccggcatcgg gtactcgcct gagtacttgc gatccagcat ggccgcggcc
    21961 gccttggccg ccgccacggg cttgaggaaa tcgtggccgt cgaagcgctc gccgcgcagc
    22021 gcgacgaaca gatcgccttc cttgatgctg cggctgtcgg tcgacacgcc ggtgaacagc
    22081 gcgtccgcgc cgctcgcgcg gccgccgagg gccgcggcgg cctccgcgag ccgcatcatc
    22141 gcgtcctccg tgcgagggcg gctcgtgcct gctcctcgtc ggagaagtga gtgcggcgcc
    22201 ccgcgatctc ctggtaggtc tcgtggcctt ttccggcgat gagaatcacg tcgtggcttt
    22261 gcgcgtccga gatcaccttc gcgatggccc gggcgcggtc cgcctcgacc tcgtgcgctg
    22321 cgctcatccc cttctcgatc tgggaaatga tcgcgagcgg gtcctcgctg cggggattgt
    22381 ccgaggtaat cagccggtaa tcggcgagac gcgcagcgat cgcccccatc agcgggcgct
    22441 tggttggatc gcggtcgccg ccggcgccga acacgacggc gaggcgcccg ccgcgctcgg
    22501 ccgcgaccgg ccgcagggtg tgaagcacgt tctccagcgc gtccggggta tgcgcgtagt
    22561 cgatcaccac cagaggctct tcgcccacgg cctgcatgcg ccccgggacc ggaggcaacg
    22621 tcgcgagcat ggctgcgcct tccttgaagc tgatcccatt tgccaccagg cagccgagca
    22681 cgccaagggc attcgccaca ttgaagcgcc cgatctgccg cagcgtcgtg gtcgccttgc
    22741 cccagctgga atggatttcg agcttcgcgc ccgcgacgcg ctcggcggcg acaaactcgt
    22801 cgacgccgac cggcttctgc gacgacaggc tgtagccgat ggttctcacg cgcccgacga
    22861 ggcgccgggc gagctcggcc ccgaacgcgt cgtcgagatt gaggaccgcg gtcccgaggc
    22921 cggggacctc gaataactgc gccttggcgc gcgcatacgc ggccatcgag ccgtggtaat
    22981 cgagatgatc gtgcgacagg ttggtgaaga gggcgcaggc aaaccgcacc ccgttgacgc
    23041 gcccctgcac caggccgtgc gaggacacct ccatcgcgac cgcgcggccg ccctggtcga
    23101 ggaagcttcg gagcaggaac tgcgtttcaa gcgcgtcggg cgtggtgttg gtggccgcgg
    23161 cgagcgcgcc cggaaagcct gcaccgagcg tcccgatcac ggcagttttc atcgcgtttg
    23221 atcccaggag cgcggcgatc cactggctgc acgaagtctt gccgttggtg ccggtgacgc
    23281 cgcacaccca gagcgactcg gagggcttgc cgtagaactc gtgcgccagc acacccgatc
    23341 gctgcttgag gtcctgaacg gggaggttgg gaacgctcca gcccgggttc cacgaaaaat
    23401 ccttgcgctc ccagagcacg gcggccgcgc cccgggcgag cgcgtcgccg atgtgcgcgc
    23461 ggccgtcggc cgcctcgccc ggataggcga ggaacgcgac gccgggcgcg cagcgccggc
    23521 tgtcggacga tagccgttcg atcgtcacgc cctgctcggc gagttgtagg aatgggtcca
    23581 tgcgaggtcc actctaggtg ctctcccgcg cttcctcgcc tccgcgcggc agctcgagcg
    23641 gcgcatcgtg cggcacgccg agcaggcgca gcgcgccctg catcacctgc gcgaacaccg
    23701 gtgccgcgac gctgccgccg tagtgctccc cggccgaggg ctcgtcgatc atcaccgcga
    23761 ccaccaggcg cggcgcgctc accggagcga aaccgacgaa agaggcgaga tatttgtcgg
    23821 gggcgtaacc gccgttctcc tgcttgtgcg cggtgccggt cttgcccgcg acccgccatc
    23881 ccatgatgcg ggcgcgcgga ccggtggcgc cgggctgcac cgaagcctcg agcatggcgc
    23941 ggaccgcccg cgccgcctcg gccgacagca ccttgtcgcc gctcaccgcc gcgcctgttt
    24001 tgaccagcgt gagcggcacc aattccccgt cgcgcgcgaa cacggtgtag gcccgggcga
    24061 gctgcgccag gctgagcgat atgccgtgcc cgtagcccat cgtggcctgc tcgatcgggc
    24121 gccagctctt gtagtgccgc agccggccgg cggcggcgcc ggggaagccg agctcgggcg
    24181 ccgcgccgaa gccgacgcgc cggaagaaat cccacatcgc ttcacgcggc agcgcgagcg
    24241 cgatcttcgc cgcgccgaca ttggaggagc gcgcgagcac ctcgccgacg gtctgcgaag
    24301 ccgccgggtg cacatcgcgg atcgtgtact tggcaagcgt gaggctgccc ggcgcggtgt
    24361 gcaccaccgt ggacgcgctc actttcccgg tctccagggc gagcgcgacg gtgaaaggct
    24421 tgagcgtcga ccccggctcg aagaggtcgg tgatcacgcg gttgcgcagc tgcgcaccgg
    24481 tgagccgcgc ccggttgttc gggttgtacg agggcgtgtt cgcgagcgcg aggatttcgc
    24541 cggtgcgcac gtccaccacg atgatcgcgc ccgccttcgc gcggtgattc tcgactgccg
    24601 ctttgagcgc gccgaatgca aggttctgga tcttgctgtc gatggagagc gcgaggtcgg
    24661 tgccgtcctg tccggagcgg atcgactcga cgtcttcgac gatccgcccg aggcggtcgc
    24721 ggatgacgcg ccggctgccc ggcttgccgc cgagcagcgc ctggtgcgcg agctcgaccc
    24781 cctcctgccc ggcgtcgtcc accccggtaa agccgatcac gtgagcggtc acttcgccgc
    24841 cggggtagta gcggcgatat tcggggtgct cgtgcacgcc cttcaggccg agcgcctgca
    24901 ccgcggccgc cgcttcgggc gccatctggc gcttgaggta gacgaagtct ttcccggtat
    24961 cggacagcct cttgtccagg tctgtctttg acatcgacaa cagcgctgcg agcttgcgcc
    25021 gatcactccc gcccgcatcg acgtcgcctg ggatcgccca caccgacttc accggggtcg
    25081 agacggcgag cgcctcgccg ttgcgatcga ggatgcgccc gcgcgtcgcc gggatctcga
    25141 ggacgcgcga gtagcgcgcg tcgcccttct cctgcaggaa gtcggtgcgt accgcctgga
    25201 ggtaggtggc gcgcgccgcg agcacggcga agccggcgac gagcatgatg agcatcacgc
    25261 gggcgcgcca caccggcagg cgcaccaggc cctgcgcggc gcgactcacc gcccggcgtc
    25321 tcgcccgacg aagcgaacgc gccgcggatc ggccgggcgc aggccgagcg agcgcgtggc
    25381 gagcctctcg acccgcgagt gcagccccca ggtgctggcc tcgagctgca ggcggccgaa
    25441 ctcggcctcg agctcgcgcg cgagcgcctg ctcgcgctcc agctgcgaga acaggcgccg
    25501 gcccttgtgc tgggtagtga cgagcccgag cgcgcaggcg acgagaaccg cgaggaggag
    25561 gaggttcagt ttcgccaccg cgcgccgggc tcgattcggt tgttgggaga aatgggggga
    25621 gcagtaaagg tgaccgcggt gcgctcggcg acgcgaagcg tggcgcttcg cgcccgcgga
    25681 tttcttgtta tttcctggcg ggacgcgcgc tgcgcacgcc ccacgattct gagagccggt
    25741 tgtggcattt cgctggcacg cagcgggagg cggcggggca tttccgggcg cgccagcgac
    25801 tgcatgaacc gcttgacgac gcggtcttcc agcgaatgga agctgatagc gaccagtcgc
    25861 ccctgcgggg cgagccgctc ggcagcccgc ggaagcatca gcgacacttc ctcaagctcc
    25921 cgattgacgt gaatccgtag agcctgaaag gtgcgcgtcg ccgggtcctg ccccggctcg
    25981 cgtgtgcgga ccgcctgagc cacgagatcg gccagttgcc gggtgcggac gattggcgcg
    26041 ccgcggcgag cagcaacaat cgccgctgca atctgtttag caaaccgttc ttcgccatag
    26101 tccgcgatca cccccctgat ttcttgttct tctgcgcggg caagccactc agcggcgctc
    26161 atcccggcct ccggatccat gcgcatgtcg agcggcccgt cgtggcgaaa cgagaagccg
    26221 cgcgccgggt cgtcgagctg cggcgaagac acgcccaagt cgaagagcat cccgtgcacc
    26281 tgcgcgggca ccatcgacag gtccgagaac ttcgccttga agaaggtgaa ccgcgggtcg
    26341 ccgatcgcgc gcgcggcttc ggctgcctgc gggtcgcgat cgagtgcgat cagccgcccg
    26401 gccgcgccca gccgctcgag gatcagccgg ctgtggccac cgcggccgaa ggtgccgtcg
    26461 acgtacaccc cgtcgggccg cagggccagc gcttcgatcg cctcgcgggc aagaaccggc
    26521 tggtgcatcg ggcctcacag cgaaaaatcc tcgatcccgg caggcggggt tgcgctgccc
    26581 gccagcgcct ggccgagctt ggcggcccat accccggcgt cccacagctc gaagtaatgg
    26641 ccctgcccga ccatcatcgc gtcacgctcc agcttggcgt gctggcgtag ggccggcgtg
    26701 agcaggatgc ggccggagcc gtccggcgtg acgtgctctt cgaagccgag cagcaagcgc
    26761 ttccaccagc tcgcttgggg atgaaaagcg gggaagctct cgaccttggc gcgtaccggc
    26821 tcccaggcag tttcggggta gatcagcacg caaccctccg ggtgcgcggt gagcaccacg
    26881 gatttcgagc cgtcgagcag tgcaccgcga tggcgcgtgg gcacagcgac gcgtcccttc
    26941 gcgtcgagcg tgatgaccgc ggctccgtgg aatgctgtcc ccatatggct tcttgtgcca
    27001 ctttttccta ccttttgcca ctctacgagg aaaagtcagc agggtcaagc tggaatttcc
    27061 aacgccgaca aggacttagg ttgttttcct caaccccaaa attgaatgga caagttttag
    27121 tactttcaat gggttattac ccatactgaa agtcgattct cacaactggc gaggaaaacg
    27181 acgggtgcca taattcacac cacccgatga tcaacaagtt cgtcgcctct ccggaagccg
    27241 ccctcgccga catcccggat ggcgccacag tcatgatcca cggcttcggc aacgccggca
    27301 tgccgaccgc gctgatcgac gcgctcatcg cgcaaggcgc gcgcgatctc accatcgtca
    27361 acaacaacgc cggcaacgcc gatgccgggc tcgcagcgct gatcggcgcg cggcgggtgc
    27421 gcaagatcct ctgctcgttt ccgcgccagg ccgattcgtg gcacttcgac aagctgtacc
    27481 gcgccggcga gatcgagctc gagctggtgc cgcagggcac gctcgccgag agcatccgcg
    27541 ccgcgggcgc gggaatcggc gcgttcttca cgcccacggc gtacggcacg aagctcgccg
    27601 agggcaagga gacgcgccgc atcggcgatc gcgactacgt gctcgagtac ccgatccacg
    27661 ccgactacgc gctgatcagg gccgatcgcg ccgaccgctg gggcaacctc acctaccgca
    27721 agagcgcgcg caatttcggg ccgctgatgg cgatggcggc gaagaccacg gtcgtgcagg
    27781 tgcgcgagac ggtggatctc ggcagcatcg acccggagca catcgtgacg ccgggcattt
    27841 tcgtgaagcg ggtcgtgcgg gtggcggcat ccctgaaaaa ggcggcgtga tgaaaaagct
    27901 cgatcggaag cagatggctg cgcgggtggc gcgcgatatc ccggaaggcg cctacgtgaa
    27961 cctcggcatc ggcctgccga cgctggtcgc cgactttctg ccgaaggggc gcgagatcat
    28021 cctgcacagc gagaacggca tcctcggcct cgggccgaag ccggcgccgg gcgacgagga
    28081 cgaggacctg atcaacgccg gcaaggaacc ggcgacgctg ctgaccggcg gcgcgtactt
    28141 ccaccacgcc gacgccttcg gcatgatccg cggccggcac ctcgacttcg ccatcctcgg
    28201 cgccttccag gtctcggtgg caggcgacct ggccaactgg cacaccggcg gccccgacga
    28261 catcccggcg gtgggcggcg cgatggacct cgcggtgggc gcgaaaaagg tgctcgtgat
    28321 gatggagcac ctcacgaaga agggcgaaag caagatcgtg gaacgctgca gctatccgct
    28381 caccggcgcg cgctgcgtcg atcggatcta caccgatctc gccgtgatcg acgtgaaggc
    28441 cgacggcctc cacgtcgtgg agtcgataga aggattgtct ttcgaggaac tccagcgcct
    28501 caccggagtt cctctaaaga aggcccttat tgcggcttga ttcccgcagc gcgcacgatg
    28561 cgctggtagt cgtcgatctc ggcgcgcacg aagcgcgcga actcctgcgg tgacatgtcg
    28621 agcggcgcgt tgccggtgtt ggcgagcttt tcgcgcacgc ccggatcggc cagtgcccgg
    28681 cgcacatcgg cgttgatttt cgcgatcagc tcggcgctca tcccggccgg cccccagacg
    28741 ccgaaccaga gcccgaagtc ggctcccttc acccccgctt ctcccgtcgt cgccagatcg
    28801 ggaagcagcg cgctgcgctt ggaggtgctc accgccaggg cgcgcagcct gccagccttg
    28861 atgttcgaga gcccggccga gatcggaacc cagtagcagt cgacgtttcc gctcaggatc
    28921 gccgcgacca cctccggcgt gcctttgaac ggcacctgcg tcacgtcgag gccggcggcg
    28981 gtgatcagct tcacggtgtt gaggtgagtg ccgctgccca cgccggcgtg gccgaaattg
    29041 atcctgctcg ggttggcttt accgaaggct acgagatccg cgagcgtctt gtaccgcgac
    29101 tcgccgctga ccaccagcac gttcggcagc tcggagagcg gcgcgatgtc cgtgaagtcc
    29161 ttggtggtct cgtaaggcag cttggcgtag atcgccggat tgaccgagtg tgcgttggag
    29221 ttgatgagca gggtgtagcc gtctggcgcg gccttcgcca ctgccgccga gccgatcgag
    29281 ccgccggcgc cggcgcggtt ctcgttcacc accggctggc cccagtactc cgcgagcttc
    29341 gccgtgacga tgcgtccgac gatgtcggtg gcgctcgccg gggtgaacga aatgatcacc
    29401 tggacagcct tgttggggta ctgcgcatgg gcgcaggaag cgaactgagc cgcccaggcg
    29461 gcgagcaatg cgaagaagat gtttttcatt ggggtttgat tccggcggcc ttgagaactt
    29521 tcgcggtgtc gtcgacttcc ttgcgcacga actgggcgaa ctcggaggac gacatcttca
    29581 tcggcagcgt gccgagcttg gtcaggcgat cggcgaggtc cgggctcgcg agcgcgcggt
    29641 tcacgtcggc gttgatcttg ttgacgatct cggcgggcat cgcggcctgg ccccacaggc
    29701 caacccacag cgtgtagtcg aagcccggca ctccctgctc ggccaccgtc ggcacgttcg
    29761 cgagcaggct cgagcgctcc gcgctcgata ccgcgagcgc gaccgccctg ccgccgttga
    29821 cgtgcggcag ggcggcgttg atcggcgccc agtagcagca cacgcgacca gcaatggtgt
    29881 cgccgattgc ctcgggcgtt cccttgtagg cgacgtggac gacgtcgatg ccggccatca
    29941 gcttgagctt ctcgaggttg aagtgcgttc cggaaccgac gccggcagag gagaagttga
    30001 gcgcgccagg cttcgctttg gccgcggtga tgaaatcgcc gagcgtcttc cagccggtgc
    30061 tcggcgccac gatcagcacg ttcgggccgc cggcgagcgg cgcgatgttg gcgaagtcct
    30121 tcagcgtgtc atagggcagc gtggcgtaga tggacgggtt ggcgacgtgc gccgaagagt
    30181 tggcgagcag cgtgtaaccg tcgggtggct cgcgcagcac cgcggcggag ccgaccgagc
    30241 cgccggcacc ggcgcggttc tcgaccacca ccggctggcc ccacagctcg gagagctttg
    30301 ccgccaccgc ccggccgacg atgtcggtcg agctcccggg cgtgaacgcg acgatgatgc
    30361 gcaccggctt gctgggaaat ccctgggcga atacccccga aacaaacgca gcgagaaaca
    30421 gtcctagaag ccgcttcatc attcctccgt tattggggcg tcaggccggc gtcgcgcatg
    30481 atcttgccga gctcgttgta ctcgcgcgcg atgaaggcgt cggattgcgc cggcgtcatc
    30541 gacatcgggt cggtgccgag cttcgccagg cgctcgacca cctccgggga gttcaacgca
    30601 cggttcacct ccgcgttgac cttggccacg atttcgggcg gggtcttcgc cggcgcgagc
    30661 agcgcgagcc agaacgtcga ttcggcgccg ggaaagccgg cctcgccgag ggtcggcacg
    30721 ttgggaagcg ccgcgacgcg cttgttggtg ctcaccgcga gcggcttcac cttgccgtcg
    30781 cgcaccgggc ccatcccgga gaccgcgggc accattgcgt actcgactcg accggccatc
    30841 gtctcggcca ggatcggcgg cgtgcccttg agcggcacgt gcaccgcctt cacgccggtc
    30901 gcgacgttga acttctccaa gctgacgtgc gccccgcttc caacgccggc ggtggcgtag
    30961 ttgtacttgc cgggattcgc ctgcagcatc gcgaccagct ccttcaccga attcaccggc
    31021 agcgtcggcg gcacaatcag cacgctcggc aggctgccga gcatggcgac ggcggaaaga
    31081 tccttcaacg tgtcgtagcc cagatccttg tagagcacgt ggttcacggc gtgccccgag
    31141 gacaccagca ccagggtata gccgtcggga tcggctcgcg agccttcgcg gatcccgatc
    31201 gatccgccgg cgccgggctt gttgtccacg acgaccggct ggccccacgc ggcgttcagg
    31261 cgctcgccga cgatgcgccc catcacgtcg gtcgcactgc cgggggtgaa cggcacgatg
    31321 atcctgatcg ccttggcggg ataggtctgc gccgatgcgc tgccgatgaa aagtgcgatt
    31381 gccagaaaaa gaaggtttct catgcgcgcc cccgcttggc ccagtagtcc ggagaagact
    31441 tgttcttctg cagcgaagcc ggatcttcgt agcccggcgc catcgatgcc gtcgtgccgg
    31501 cgagcacctg gtggtgctcg atgttgagca ggcgcagcca gctctgctgc cccatggtga
    31561 gcgcgatgaa gcagccgagc tcgaccagct cgggctcgct gaagtgccgg tgcagccgct
    31621 cccagaacgc gtcgtcggtg tcgaggtgcc aggtgatcgc ctcggcgtag gcgagcgccg
    31681 ccttctgctt gccggagtag cgcgtggatt tctcgaagtt gagcaggtcc ttgacgtcgg
    31741 cctcgatcat cccggcgctc gcggccttca tcgagcgctg gttgccgcag tagtcgcact
    31801 gcaccgagcg cgacacgtag aggcggcaca gctccttgat gggatgctcg cacacgccgt
    31861 tgcgaaagag atcgcgccac gagttggcga acgaccagaa tgccgccggc acgtgcgcgc
    31921 gcaccgccga gctctcgggc cgcggcgtgc cttcgcgccg gcagcggtcc atctccttct
    31981 gcatctccgc gtccatcttc tcgaccggaa cgtagctgat gcgctgcttg ggcgtcatgg
    32041 cgtcctcctc cagcatcaat ttaccatcac gggatgtacc aggaccacac cgccctgcag
    32101 gccgccgggc agctgctgct cgcattcgcc ttcctcgcca ccggggtgcg gaacgcgggg
    32161 tggaagtttc gccagcatct cgaccggatg gtcgcctatg gggtgccgca ggcgaggctc
    32221 gccctcctcg ccgggttcgt gctgcagttc gtcggcggcg tgctcttgct cttcgactgg
    32281 aaccgtacgc tcggcgcggg cctgttgatc gcgttcacgg tcctcgcgag cgcgatcttc
    32341 caccgctggt ggctcgcccc cgacccgctg cttgcgcacc tgcacctctc caacctgatc
    32401 gtgaactgcg gcgtcatcgg cgggctgctg ctggtcgcgg ctatttgaag accaggctga
    32461 gctgcggcac gaacacgagg agcagcgtga ccagcgccat gcagactacg aacgggaacg
//
LOCUS       DATBSZ010000138        10030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_634527, whole genome shotgun sequence.
ACCESSION   DATBSZ010000138 DATBSZ010000000
VERSION     DATBSZ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634527"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VIG70_09045"
     CDS             complement(<1..975)
                     /locus_tag="VIG70_09045"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY8250740.1"
                     /translation="MTSTLRRVAVLGGTRIPFARANGAYMEAGNQAMLTAAMTALVDK
                     FDLRKRKVGEVAAGAVIKHSRDWNLARESTLGSGLHPETPAYDVQRACGTSLTAAVQV
                     AHRIAAGEIDCGIAGGVDSASDIPLTYGPELQKIVLSSARGRSFLEKTQPWLEVRPGH
                     LKPSAPGIAEPRTGLSMGQHAEETAKEWRVGRAEQDELALASHRNAAAAWKAGFFEDL
                     VVPFNDLRHDNNVRPDTTLEKLAALRTAFDRSSAGTLTAGNSSPLTDGAASVLLSSEE
                     FARARGWPVLAYLTHSETAAVDFVGMAGPKEGMLMAPAYAVPRMLDRAG"
     gene            1109..1585
                     /locus_tag="VIG70_09050"
     CDS             1109..1585
                     /locus_tag="VIG70_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250741.1"
                     /translation="MKRHFMTAAVLVLVSGAAIAKCKPVVGHFEAQVIPPGQGHCPMT
                     APFCTAGRVWGGIQGSYQFVMSGVVPSAEIGGVATIQFFAGKSTIFLKRGDELLGTDT
                     GSIDLPPGQGGFASLITFDGGTGKMRGATGQARLRGEFDAVEGTTSGDYIGSLCTH"
     gene            complement(1586..2413)
                     /locus_tag="VIG70_09055"
     CDS             complement(1586..2413)
                     /locus_tag="VIG70_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY8250742.1"
                     /translation="MRTQAEKAAAFRALHQRAGAFIIPNPWDAGTAKLLAAAGFEALA
                     TTSLGVSSALGRPDGAGAVSRRELLDNCAAIAAATDLPVSADLENGYAHEPRAAAEMI
                     RLAAQAGVVGGSIEDATGDEAKPIYDFAAAVERVQAAVETARSLGFPFVLTARAENFL
                     HGRRDLDDTIRRLQAFEKAGADVLYAPGLRDLETIRTVASSVTRPLNVVIGMIDPAIT
                     AAELAQAGVKRISVGGALCRLALAHMMRGAREMKERGSFAWLAEAIPGSELRRTFGG"
     gene            2434..3726
                     /locus_tag="VIG70_09060"
     CDS             2434..3726
                     /locus_tag="VIG70_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY8250743.1"
                     /translation="MEEALLLARVQFGLNIAFHILFPTITIGLAWILVVFRLRYERTR
                     DPAWLGAYKLWVKVFALTFAMGVVSGITMSFQFGTNWPGYMNKVGNVAGPLLAYEVLT
                     AFFLEATFLGVMLFGMNRVSGRVHAASAVVVAFGTTVSAFWILALNSWMQTPTGHVVQ
                     DGVLIAGSWLAVIFNPSFPYRFAHMLLASAITAAFVVAGLSAWRLLRAANDASARKTL
                     RFAVRLAALLVPLQIVVGDLHGLNTLEHQPAKIAAVEALWKTEKGAALTLFAVINEEK
                     KRNDYAVEIPKALSLILKHDLDGVVRGLEEFGKDHPPVAPVFWSFRVMVGVGVLMLVL
                     SWVGVFVLRKSTPRWLLWSFAAFTFSGWIATLAGWIVTEIGRQPWLVTGILRTADAVG
                     QASGAQLGASLTGYVLTYAALLISYMVVLTHLAGKGAQ"
     gene            3860..4837
                     /locus_tag="VIG70_09065"
     CDS             3860..4837
                     /locus_tag="VIG70_09065"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY8250744.1"
                     /translation="MNIDLPVVFTVLMGMAILAYVVLDGYDLGVGMLMPAADRDEQNR
                     MVASIGPFWDANETWLVLGIGLLLVAFPLAHGEILGALYLPVSAMLVGLMLRGVAFEL
                     RIKAEGWHKELWNWLFWAGSTLASLSQGFMLGRYITGFAPGMGYWLFALIVAASLCGG
                     YVLLGATWLVLRTDGPLQAKAVAWARWGLLWVALGVALVSLGTPLVSETVRDKWFDFP
                     RTAALMVLPGGTLAMWLWVWTCLGKSDWKPFAGAVAIYVLAFIGLAYSLFPFVIMDRL
                     TIWDAAAHPSALVFMLVGTAIVLPFIVGYTIYAYRVFSGKVTGGDPYER"
     gene            4827..6023
                     /locus_tag="VIG70_09070"
     CDS             4827..6023
                     /locus_tag="VIG70_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEY8250745.1"
                     /translation="MKDESRRSFITAAASAGALASLPLKAFAQTEPPGTISNDVIKGE
                     KAKSMQWHSERPLTGSVPAHEHDFDATPNDRMFVRNNLTTPAIDINTHRLTIKGLVDK
                     ELSFSTFDLAKEFRTVSMQGMLECAGSGRSTYRPAASGTPWLETGGMGCPVWGGVRLA
                     DLLKAAGLKPGAAHVAGQGGDPGMVPTAAPVIRSIPMAKAMNEGTLVAWAMNGEPLPW
                     VHGYPLRLVVPGWVGSASTKWLHTLTVLDAPFKGTYMDSSYRVPRTPIEPGTKMPADA
                     VSAEAWPVKSIITSPAPNAHVKSGPILVRGRAWVGEGTVERVEISSDEGKTWQRAQLA
                     PASPLHPHAWRTFTYEHRAQRPGYVSFLARAWDDRGNSQPAVPYWNPLGYFWNGWHRV
                     GVVVEA"
     gene            6040..6576
                     /locus_tag="VIG70_09075"
     CDS             6040..6576
                     /locus_tag="VIG70_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8250746.1"
                     /translation="MTGFFLAGEAQDKLVSGPGSTLTETKCKICHELQHIRRAPLSRG
                     EWADNLKNMKERGTPMTDAEMAVILDYLATYYNRERPAPEPSPDTLATASADPIEKLL
                     GAHACAACHALDKRVVGPSFREVAAKYAGDAGAAERLAGKIRAGGQGAWGSVPMPPNT
                     GLSEADAKTLAGWVLQQK"
     gene            complement(6622..6864)
                     /locus_tag="VIG70_09080"
     CDS             complement(6622..6864)
                     /locus_tag="VIG70_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY8250747.1"
                     /translation="MEQGRLAFTAVYLKSNHGYIGFIEELPGVNSHGRTLDEARATLQ
                     KLAAVVFDEERREAEELIAGKDVVRENFVVPIPRAA"
     gene            complement(7073..7396)
                     /gene="fdxA"
                     /locus_tag="VIG70_09085"
     CDS             complement(7073..7396)
                     /gene="fdxA"
                     /locus_tag="VIG70_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEY8250748.1"
                     /translation="MTYVVTESCIKCKYTDCVDVCPVDCFREGPNMLVIDPDECIDCT
                     LCVPECPVEAIFAEDDVPDAQKAFTALNADLAKVWKPIIERKPAPPDADEWAKVKDKK
                     NLLQR"
     gene            7495..7830
                     /gene="trxA"
                     /locus_tag="VIG70_09090"
     CDS             7495..7830
                     /gene="trxA"
                     /locus_tag="VIG70_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEY8250749.1"
                     /translation="MASTTSSIHRVTDDTFEPEVLKSETPVLVDYWAEWCGPCKSIAP
                     ILDEVAKEYEGRLKIAKINVDENQLTPAKFGIRGIPTLMLFKNGNVEATRVGALSKSQ
                     LTAFLDSNI"
     gene            8040..9299
                     /gene="rho"
                     /locus_tag="VIG70_09095"
     CDS             8040..9299
                     /gene="rho"
                     /locus_tag="VIG70_09095"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY8250750.1"
                     /translation="MHLSELKTHHVTQLVEMAVSVGIENASRLRKQDLIFALLKNQAK
                     KGASIFGDGTLEVLPDGFGFLRSPDTSYLASTDDIYVSPSQIRRFNLHTGDSIEGEIR
                     TPKDGERYFALVKVDKVNGEAPEAAKNKILFENLTPLHPDKPFKLEREIKAEENITGR
                     VLDIVAPVGKGQRGLIVSPPKAGKTVMLQHIAHALVANHPEIALIVLLIDERPEEVTE
                     MSRSVRGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQKPKRFFGAARNVEEGGSLTIIATALIDTGSRMDDVIYEE
                     FKGTGNLEVHLDRRMHEKRIYPAINVNRSGTRREELLIEAPILQKVWVLRKLLFPMDE
                     LEAAEFLIDKLKATKSNAEFFDSMRRG"
     gene            complement(9346..9717)
                     /locus_tag="VIG70_09100"
     CDS             complement(9346..9717)
                     /locus_tag="VIG70_09100"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEY8250751.1"
                     /translation="MSSGRHFIPYRRDLKRRSQSLRRDPSPAERKLWYEFLRGLSVKF
                     TRQKPLGRYIADFYCASRMLVVELDGDSHYNDRAQRYDAFRTSELGAQGIQVVRVTNA
                     DVMQNFEGVCAMILRILDTKP"
BASE COUNT         1654 a         3346 c         3290 g         1740 t
ORIGIN      
        1 gccggcgcga tcgagcatgc gcggcacggc gtaggccggc gccatcagca tgccttcctt
       61 gggacccgcc atgccgacaa agtccaccgc ggcggtttcg gaatgggtca ggtaggcaag
      121 aaccggccaa ccccgggcac gcgcaaattc ttctgacgaa aggagcacgg acgctgcgcc
      181 atcggtgagc ggcgagctgt tgcccgcggt gagcgtgccc gccgaagacc tgtcgaaggc
      241 cgtgcgcaga gcggcgagct tctcgagcgt ggtatcgggc cgcacgttgt tgtcgtgccg
      301 caggtcgttg aacggaacca ccaggtcctc gaagaagccc gccttccacg ccgccgcagc
      361 gttgcggtgg ctggcgagcg cgagctcgtc ctgctcggcg cggccgacgc gccattcctt
      421 tgcggtttcc tcggcgtgct gccccatcga caggccggtg cgcggctcgg cgattcccgg
      481 cgccgagggc ttcaggtgac ccgggcgcac ctccagccag ggttgcgtct tctcgaggaa
      541 agagcggccg cgggcgctcg aaagaacgat cttctgcagc tccggcccat aggtgagcgg
      601 gatgtcgctc gccgaatcga cgccgccggc gatgccgcag tcgatctcgc ccgccgcaat
      661 gcggtgcgcc acctgcaccg ccgcggtgag gctcgtgccg caggcgcgct gtacgtcgta
      721 ggccggcgtc tcgggatgca ggccgctgcc gagcgtcgat tcgcgcgcga gattccagtc
      781 gcgcgagtgc ttgatcaccg cgccggcggc gacttccccg accttgcgtt ttctgagatc
      841 gaatttatcg acgagtgcag tcatggcggc cgtgagcatg gcctggttgc cggcctccat
      901 gtaggcgccg ttcgcgcgcg cgaacgggat gcgagtgccg ccgaggacgg ctacccggcg
      961 cagcgtgctt gtcatgggaa gactctaagc cttttcgagg tgcgtaatta tgtcgccgcg
     1021 ggcgagcgcc ataggttacc gcctgactcc ggatacccgc gccacaatca aatcgcagcc
     1081 aatttctcaa ccgtgaggga ggaaacccat gaaaaggcac ttcatgacgg cagcggttct
     1141 cgtcctggta tcgggtgcgg cgattgcgaa gtgcaagccg gtggtcgggc atttcgaggc
     1201 gcaggtcatt cccccgggcc agggccattg cccgatgacg gccccgttct gcaccgcggg
     1261 ccgggtctgg ggcggcatcc agggcagcta ccagttcgtg atgagcgggg tcgtgccctc
     1321 ggcggagatc ggcggcgtcg ccacgatcca gttcttcgcc gggaagagca cgatcttcct
     1381 caagcgcggg gacgagcttc tcggcaccga caccggcagc atcgacctgc cgccgggcca
     1441 gggcggtttc gcctcgctca tcacctttga cggcggtacc gggaaaatga ggggcgccac
     1501 cggccaggcg cggctacgcg gcgagttcga cgcggtggaa ggcacgacca gcggcgacta
     1561 tatcggcagc ctctgtacgc actaactagc cgccgaaggt tcttctcagc tcgctgcccg
     1621 ggatcgcctc ggccagccag gcaaagctgc cgcgctcctt catttcgcgc gcgccgcgca
     1681 tcatgtgcgc gagcgcgagg cggcataggg cgccgccgac gctgatccgc ttgacgccgg
     1741 cctgcgccag ctcggccgcg gtgatggccg ggtcgatcat gccgatcacg acgttgagcg
     1801 gacgcgtgac cgaggacgcc acggtgcgga tcgtctccag gtctctcagc cccggcgcgt
     1861 agaggacgtc cgcccccgct ttctcgaacg cctgcaggcg gcggatcgtg tcgtcgaggt
     1921 cgcgccggcc gtgcaggaag ttctcggcgc gcgcggtcag cacgaacgga aagccgagcg
     1981 agcgcgcggt ctcgaccgcg gcctgcaccc gctccaccgc agccgcgaag tcgtagatgg
     2041 gcttcgcctc gtcgccagtc gcgtcctcga tcgagccccc gaccaccccg gcctgcgccg
     2101 cgagccggat catctccgcg gcggcgcgcg gctcgtgcgc atagccgttc tcgaggtccg
     2161 cgctcaccgg caggtcggtc gccgcggcga tcgcggcgca gttgtcgagc agctcgcgcc
     2221 ggcttaccgc gccggcgccg tcgggccgcc ccaacgcgct cgagaccccc aggctggtcg
     2281 tcgccagcgc ctcgaacccg gccgcggcaa gcagcttcgc ggtgccggcg tcccacggat
     2341 tcggaatgat gaacgcgccg gcgcgctggt gcagcgcccg gaatgcggcg gctttttctg
     2401 cctgggttct catgccggta tattaggccg actatggaag aagccctcct gctcgcgcgg
     2461 gtgcagttcg ggctgaatat cgcctttcat atcctgttcc cgaccatcac catcgggctc
     2521 gcctggatcc tggtggtgtt ccggctgcgc tacgagcgca cgcgggaccc ggcctggctc
     2581 ggcgcctaca agctctgggt gaaggtgttc gcgctcacgt tcgccatggg cgtggtctcg
     2641 ggcatcacca tgagcttcca gttcggcacc aactggccgg gctacatgaa caaggtgggc
     2701 aacgtcgccg ggccgctcct cgcctacgag gtgctcaccg cgttcttcct cgaggcgacg
     2761 ttccttggcg tgatgctgtt cggcatgaac cgggtctcgg ggcgcgtgca cgcggcttcg
     2821 gcggtcgtcg tcgccttcgg caccaccgtg tcggcgttct ggatcctggc gctcaattcc
     2881 tggatgcaga cgccgaccgg ccacgtggtg caggatggcg tgctcatcgc cgggagctgg
     2941 ctcgcggtga tcttcaaccc gtcgttcccc taccgcttcg cgcacatgct gctcgcctcg
     3001 gcgatcaccg cggcgttcgt cgtcgccggc ctctcggcct ggcggctcct cagggcggcg
     3061 aacgacgcct cggcgcgcaa gacgctgcgc ttcgccgtgc gcctcgccgc gctgctcgtg
     3121 ccgctgcaga tcgtggtggg cgacctgcac ggcctgaaca cgctcgagca ccagcccgcg
     3181 aagatcgccg cggtcgaggc gctgtggaag accgagaagg gcgcggcgct gacgctgttc
     3241 gcagtgatca acgaagaaaa aaagcgcaac gactacgccg tcgagattcc caaagcgctc
     3301 tcgcttatcc tcaagcacga cctcgacggc gtggtgcgcg gcctggagga gttcggaaag
     3361 gaccaccccc cggtggcgcc ggtgttctgg tcgtttcgcg tgatggtcgg cgtgggcgtg
     3421 ctgatgctgg tgctctcctg ggtgggggtt ttcgttctga gaaaatcaac cccccgctgg
     3481 ctgttgtggt ccttcgccgc cttcaccttc tcaggctgga tcgcgacgct cgcgggctgg
     3541 atcgtcaccg agatcggccg ccagccctgg ctcgtcaccg gcatcctgcg cacggcggac
     3601 gcggtcggcc aggcgagcgg cgcgcagctc ggcgccagcc tgacggggta tgtattgacc
     3661 tacgcggccc tgctcatctc ctacatggtg gtcctcacgc atctcgcggg gaaaggcgcc
     3721 caatgagagc tgcgtttctt tgcccccctg acaagggggg aagcgagcga gggtttcgcg
     3781 agcggggggt ttcgccctcg ccaaaacaaa accccccgct cgcctccggc tcacgccccc
     3841 cttatcaggg ggtcacggca tgaacatcga cctgccagtc gtgttcacgg tgctgatggg
     3901 catggcgatc ctcgcctatg tggtgctcga cggctacgac ctcggcgtgg gcatgctgat
     3961 gccggcggcg gaccgcgacg agcagaaccg catggtcgcc tcgatcgggc cgttctggga
     4021 cgcgaacgag acctggctgg tgctcggcat cgggcttctc ctggtcgcgt tcccgctggc
     4081 gcacggcgaa atcctcggcg cgctgtacct gccggtgtcg gcgatgctcg tcgggctgat
     4141 gctccgcggc gtggccttcg aattgaggat caaggcagag ggctggcaca aggagctctg
     4201 gaactggctg ttctgggcgg gctcgacgct ggcaagcctg tcgcagggct tcatgctcgg
     4261 gcgctacatc accggcttcg cgccgggcat gggctactgg ctgttcgcgc tcatcgtcgc
     4321 cgcgagcctg tgcggcggct atgtgctgct cggagcgacc tggctcgtgc tgcgcaccga
     4381 tggcccactg caggcgaaag cggtggcgtg ggcgcgctgg gggctgctgt gggtggcgct
     4441 cggcgtcgcg ctggtgagcc tcggcacgcc gctcgtcagc gagaccgtac gcgacaagtg
     4501 gttcgacttc ccgcgcacgg ctgcgctgat ggtgctgccc ggggggacac tcgccatgtg
     4561 gctctgggta tggacctgcc tgggcaagtc ggactggaag cccttcgccg gcgcggtcgc
     4621 catctacgtg ctcgcgttca tcggcctcgc ctacagcctg ttcccgttcg tcatcatgga
     4681 ccggctcacg atctgggatg cggccgcgca cccgagcgcg ctcgtcttca tgctcgtggg
     4741 caccgcaatc gtgctgccgt tcatcgttgg ctacactatc tacgcctatc gagtattcag
     4801 cggcaaagtg accggaggag atccctatga aagatgagtc gcgacgtagt ttcattaccg
     4861 cggcggcaag cgccggcgcc cttgcttcgc ttcccctgaa agcgttcgcg cagaccgagc
     4921 ccccgggcac catttccaac gatgtcatca agggcgagaa ggcgaagagc atgcaatggc
     4981 actcggagcg cccgctcacc ggcagcgtgc cggcgcacga gcacgacttc gacgcgacgc
     5041 cgaacgatcg catgttcgtc cgcaacaacc tcaccacgcc cgcgatcgac atcaacaccc
     5101 accgcctgac gatcaagggg ctggtcgaca aggagctgag cttttccacc ttcgacctgg
     5161 cgaaggaatt caggaccgtc tcgatgcagg gcatgctcga gtgcgcgggc tcgggccgct
     5221 cgacctaccg ccccgcggcg agcggcactc cctggctcga gaccggcggc atgggctgcc
     5281 cggtgtgggg cggcgtgcgg ctcgccgacc tcctcaaggc cgcgggcctc aagcccggcg
     5341 cggcgcacgt cgccggccag ggcggcgacc ccggcatggt gccgaccgcg gcgccggtga
     5401 tccgctcgat cccgatggcg aaggcgatga acgagggcac gctcgtcgcc tgggcgatga
     5461 acggcgagcc cctgccctgg gtgcacggct atccgctgcg cctggtggtg ccgggatggg
     5521 tcggctcggc ctcgaccaag tggctgcaca cactgaccgt gctcgacgcg ccgttcaagg
     5581 gcacatacat ggactcgagc taccgcgtgc cgcgcacgcc gatcgagccc ggcacgaaga
     5641 tgccggccga tgcggtgtcg gcggaagcct ggccggtgaa gtccatcatc acctcgccgg
     5701 cgccgaacgc gcacgtgaag agcggcccga tcctggtgcg cggccgcgcc tgggtgggcg
     5761 aaggcacggt cgagcgcgtc gagatctcga gcgacgaagg caagacctgg cagcgcgcgc
     5821 agctcgcgcc cgcctccccg ctacacccgc acgcctggcg cacgttcacc tacgagcacc
     5881 gcgcgcagcg tcccggctac gtcagcttcc ttgcgcgcgc ttgggacgac cgcggcaact
     5941 cgcagcccgc ggtgccgtac tggaatccgc tcggctactt ctggaacggc tggcatcgcg
     6001 tgggggtcgt ggttgaggcg tagcctcgct tttctcgcct tgacggggtt ttttcttgct
     6061 ggagaagcgc aagacaagct cgtctcgggg ccgggctcca ccctcaccga gaccaagtgc
     6121 aagatctgcc acgagctgca gcacatccgg cgcgcgccgc tctcgcgcgg cgagtgggcc
     6181 gataacctga agaacatgaa ggagcgcggc acgccgatga ccgacgccga aatggcggtg
     6241 atcctcgact acctggcgac ctactacaac cgcgaaagac cggcgcccga gccctcgccc
     6301 gatacgctcg ccaccgcgtc ggcggacccg atcgagaagc tcctcggcgc gcacgcctgc
     6361 gccgcctgcc acgcgctcga caagcgcgtg gtcggcccgt cgttcaggga agtcgcggcg
     6421 aagtatgccg gcgacgccgg cgccgcggag cggctcgccg ggaaaatccg cgcaggcggc
     6481 cagggcgcgt ggggcagcgt gccgatgccg ccgaataccg gcttatcgga agcggatgct
     6541 aaaacgctgg cgggctgggt gctgcagcag aaatgagaag ggcgccagcg gcgccccttt
     6601 tcagactcca atcgaaaagc gctaggcggc gcgaggaatc ggcaccacga agttctcgcg
     6661 caccacgtcc ttgccggcga tcagctcttc cgcttcgcgg cgctcttcgt cgaacacgac
     6721 cgcggcgagc ttctgcagcg tcgcgcgcgc ttcgtccagc gtgcggccgt gcgagttcac
     6781 tccgggaagt tcctcgatga atccgatgta gccatggttc gacttgagat aaacggcggt
     6841 gaacgcgagc ctgccttgtt ccatcattcc tcctgccggg gcgtgaagcg tttctatatc
     6901 gacaggttac gcgatcaagc ttgcaaaagt atgactcgcg aaagaaaact tcggcataag
     6961 ttgatacttc aaaatcgctg tggtcattac gacgacacat tttgcgttgc gcaaaatttg
     7021 ttgcactgca tttccgaatt gctgcggtgc ttttttctct ctcttatctc ggctacctct
     7081 gcagaagatt ttttttatcc ttgactttcg cccactcgtc cgcgtcgggc ggcgcgggct
     7141 tgcgctcgat gatcggcttc cacactttcg cgagatccgc gttcaacgcc gtgaacgcct
     7201 tctgcgcatc gggcacgtcg tcctccgcga agatcgcctc caccgggcac tcgggcacgc
     7261 acagcgtgca gtcgatgcac tcgtcggggt cgatcaccag catgttcggc ccctcgcgga
     7321 agcagtcgac cgggcacacg tcgacgcagt cggtgtactt gcacttgatg caggactcgg
     7381 tcaccacgta ggtcattaga atcaatagtt tagcacctcc tcggtcgacg acttgggaac
     7441 cacctgcgtc gaccccataa tagaagtctt cctgcaacat ccccttgagg agccatggcg
     7501 agcaccactt cttcgattca tcgcgtgacc gatgacacgt tcgagcccga ggtcctgaag
     7561 tccgagaccc cggtgctggt cgattactgg gccgagtggt gcggcccgtg caagtcgatc
     7621 gcgccgatcc tcgacgaggt ggcgaaggag tacgaaggcc gcctcaagat cgcgaagatc
     7681 aacgtcgacg agaaccagct cacgccggcg aagttcggca tccgcggcat cccgacgctg
     7741 atgctgttca agaacggcaa cgtcgaggcg acccgcgtcg gggcgctgtc gaagtcccag
     7801 ttgaccgcct ttcttgacag caacatctga agcttctagt ctgtacgcgt aggtgagccc
     7861 cccgtcctca aagggggcgg ggttctagca acaaaattcc cgaacaaggt tgtccgcgcc
     7921 tcagaaattc gggcgcacat ttcccaagtc ctatggccag acctcaagcg gtcccgcccg
     7981 aagcagcaac gcctgcagcc ccgcgccccg agtccgacgg cgcgccgcca gcgattccgc
     8041 tgcacctgtc ggagctcaag acccaccacg tcacgcagct cgtcgagatg gcggtttcgg
     8101 tcggcatcga gaacgcgagc cggttgcgca agcaggacct gatcttcgcg ctgctcaaga
     8161 accaggcgaa gaaaggcgcg tccatcttcg gcgacggcac cctggaggtg ctgcccgacg
     8221 gcttcggctt cctgcgctcc ccggacactt cctacctcgc gagcaccgac gacatctacg
     8281 tctcgccctc gcagatccgg cgcttcaacc tgcataccgg cgactcgatc gagggcgaga
     8341 tccgcacgcc gaaggacggc gagcgctatt tcgccctggt caaggtcgac aaggtcaacg
     8401 gcgaggcccc cgaggcggcg aagaacaaga tcctgttcga gaacctgacg ccgctgcacc
     8461 cggacaagcc gttcaagctc gagcgcgaga tcaaggccga agagaacatc accgggcggg
     8521 tgctcgacat cgtcgccccg gtcggcaagg gccagcgggg cttgatcgtg agcccgccga
     8581 aggccggcaa gaccgtgatg ctgcagcaca tcgcgcacgc gctggtcgcc aaccaccccg
     8641 agatcgcgct gatcgtgctc ctgatcgacg agcggcccga ggaagtgacc gagatgtcgc
     8701 gctcggtgcg cggcgaagtg gtcgcctcca ccttcgacga gccggcctcg cgccacgtgc
     8761 aggtcgccga gatggtgatc gaaaaagcga agcgcctggt cgagcacaag aaggacgtgg
     8821 tgatcctgct cgattcgatc acccgcctcg cgcgcgcgta caacacggtc gtcccggcgt
     8881 ccggaaaagt gctgaccggc ggcgtggacg cgaatgcgct gcagaagccc aagcgcttct
     8941 tcggcgcggc gcgcaacgtc gaggaaggcg gctcgctcac catcatcgcc accgcgctga
     9001 tcgacaccgg cagccgcatg gacgacgtga tctacgagga gttcaagggc accggcaacc
     9061 tggaagtgca cctcgaccgg cgcatgcacg agaagcgcat ctacccggcg atcaacgtca
     9121 accgctccgg cacgcgccgc gaggagctgc tgatcgaggc gccgatcctg cagaaggtgt
     9181 gggtattgag gaaacttctc ttcccgatgg acgagctcga ggccgccgaa ttcctcatcg
     9241 acaagctgaa ggccaccaag tcgaacgcgg agtttttcga ctcgatgcgc cgagggtagg
     9301 ttttgccccc ctgataaggg gggagcgagc ttgcgagcgg ggggtttagg gtttggtgtc
     9361 gaggatccgc aggatcatcg cgcagacccc ttcgaaattc tgcatcacat ccgcgttcgt
     9421 aacacgtacc acctgaatgc cctgcgcgcc tagctccgaa gttcggaacg cgtcgtatcg
     9481 ttgcgcacga tcgttgtaat ggctgtcccc gtccagctcg accacgagca tccggctagc
     9541 gcagtagaaa tccgcgatgt agcgtcccag gggcttttgt cgggtgaatt tcacggagag
     9601 accgcgtagg aactcgtacc aaagcttccg ctctgcgggg cttggatcgc gccgtaacga
     9661 ctgcgaccgg cgcttcaggt cacggcgata aggaatgaag tgacggccgg atgacattcc
     9721 caagcaacgc gcctgacgcg tttcggctta ctaaaacccc ccgctcgctt cgctcgcgac
     9781 ccccttatca gggggtcatt tagaatcggt tcaaggggga cgcatgctcg acaccacacg
     9841 tcgagtcgca acacctgaag gaatcgaact cacgttgcgc ctcgccggtc cggtgccgcg
     9901 cgcgctcgcc tgggcgatcg acttcctgct gcgtgcggcg ctggtgctga tggtgcagat
     9961 gatcgccgcg ttcttcgggc gcgccggcat gggcgtggtg ctgatcgccg cgttcttcgc
    10021 cgagtggctg
//
LOCUS       DATBSZ010000139        21012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_703478, whole genome shotgun sequence.
ACCESSION   DATBSZ010000139 DATBSZ010000000
VERSION     DATBSZ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21012
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703478"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VIG70_09105"
     CDS             <1..991
                     /locus_tag="VIG70_09105"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEY8250752.1"
                     /translation="DLVGAPIAAAKLSQATASAVAACDAADGVVDGVIDDPRTCKFSA
                     TANVCGTATAPAANCLTLQEADAIDLIWDGPRNARGSKIWFGLDRGTNLSGLNGTNPF
                     ALGVTQFHWDMHDRNFDWRTVTMATYADVAQAGSRNIADVTDTFGDLDTFRRSGGKLL
                     TFVGANDQLIMPRGVINYYREMAARDGKHDEPDFDRLQRFYRLFRAPGVAHCGGGAGP
                     QPQNLFGALVDWVEKGIAPEQILGLQNLGGGLTRTRPICLYPQTAIYNGTGSTDDAAN
                     FHCGGNLERRRTVCADALAKYKHEVNGRLDFDGTGLDREDCDGRRHHHDHWHW"
     gene            1072..1165
                     /locus_tag="VIG70_09110"
     tRNA            1072..1165
                     /locus_tag="VIG70_09110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1106..1108,aa:Ser,seq:gct)
     gene            1184..2146
                     /locus_tag="VIG70_09115"
     CDS             1184..2146
                     /locus_tag="VIG70_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEY8250753.1"
                     /translation="MSLGRVLVEKFQPDVLEWLRARAEVVVVDPWAEPDRWEAEAPAV
                     DAVISRKGRITRQHMEKSHGRLKIVARTGVGFDASRIDVDAAKEHKVWVTNMPGSNAI
                     AVAELAMGQLVALARHTMAANRAVKENRWGGDYLRLIGVELAGKTLGIVGMGNIGTRV
                     ALRARAFEMSLLVCDPYIPASHVTSLGGRWAGLDEVFGEADFVTLHCPLNRETRHLVG
                     ERQLSRMKRSAYLVNLARGGVVDEEALYKCLSEKRIAGAAIDVLETEPPRRDHPLIGL
                     DNVIFSPHIGGSAREATSRGERGAAEEVVRVLEGKSPLNPVFQK"
     gene            2203..3354
                     /locus_tag="VIG70_09120"
     CDS             2203..3354
                     /locus_tag="VIG70_09120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEY8250754.1"
                     /translation="MAKDLYRVGITGSYGGLNLGDEAILQSIIAQLRKDLPKLEITVF
                     SRDADDTKRRHQVERAVPVRKLSRAEIMPEVERLDLLILGGGGILYDADARTYLREVQ
                     LAREKRVPVFVYAIGAGPLTHAAAQAAVRENLADVEMVTVREKSAHRVLEESGMHRDD
                     VVVTADPALLLKPEPLPRGILRHEGLEGRRRLIGMSVREPGVAAPDLDEKVYHALLAN
                     AADFMVDRWNANVVFVPMERSVLDTQHSHAVIAKMLRAQRATVLKGEYTAGQVLSWMG
                     KFDFALGMRLHFLIFAAIQGTPFVALPYAGKVSGFLEALGVPTPPLNLVNAGRLIAYL
                     DDSWDRRRSMRTRIAESLPALQERARETHRLLLEVLTGEKSRNAHIKAA"
     gene            3332..4765
                     /locus_tag="VIG70_09125"
     CDS             3332..4765
                     /locus_tag="VIG70_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8250755.1"
                     /translation="MPTLKRPDDAPSIELPPRQALLAGEADVLVVGGGPAGLGAALGA
                     VQAGARVILAERYGFLGGNATAALVMPLMSFHTQTPTPERRGATTLLPTDHGPGEAVV
                     GGVLAKLLARLVSVGGAIPPTLATGYVVPFDPEWFKIVALDLLDEAGVQILFHAFASG
                     VLGENRVEGVVFETKSGPLAIRAKVTIDCTGDADLAVQAGTPTETGRADGLVQPMTLM
                     FRMAEFHRAAFEGYVKENPKQWRGVHGLWDLVREATAAGALKLPREDILFFATPHEGE
                     VSVNSTRVTRVLGTDVWDLSYAEWMSRRQMRQIAEFLKQYVPGFEKSYVMQSGVNVGV
                     RETRRIIGDYQLTVDDVLSARKFDDGIARGAYPVDIHNPAGTGTVLKRLPPGEAYDIP
                     LRCLMPKNAEGLVVAGRCISGTHEAHSSYRVMPIVMATGQAAGITAAIAARRGARPRE
                     ISVKEVQYEIVRQGGSLRKELHPAKAA"
     gene            4820..6250
                     /locus_tag="VIG70_09130"
     CDS             4820..6250
                     /locus_tag="VIG70_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8250756.1"
                     /translation="MTRESELRVVTDAMPAAAVRCARDQTFLWVNPRYASWMARPADA
                     IIGRKLAEVIGREAMYTIEPFIQRVLKGEQVQYERLARFAGLGERWVGWMYTPTPDGW
                     VAIGTDIHDRKLAEEALKAEHQRKDEFLAALAHQLRNPLAPIRNAVAILARKGSQDPE
                     VAWSQAVIERQIEQLSRLIGDLVDVERIARGNLALRRERVTLEAVVDMALESSRPQVN
                     AGGHRLSVLLPSERAMLDADPARLAQALATLLKNAAKHTPPQGTITLSAAVEGGEVVV
                     AVEDSGVGFDAPAARSLFVKEAGVGLRLVHGIVALHRGRIEARSAGPGRGAEFVVRLP
                     RASGVSRPATTSPHYTGYSAAAPVRVLVADDNRDAADSLQRVLALYGHEVRVAYDGAT
                     ALRIGQEFRPRVAVLDIAMPGSDGYQVAQAIRRQQGREVTLVALTGWGQEADRRRAME
                     SGFDHHLVKPVDPNALNALLAEVIEK"
     gene            complement(6251..6547)
                     /locus_tag="VIG70_09135"
     CDS             complement(6251..6547)
                     /locus_tag="VIG70_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250757.1"
                     /translation="MPGRKKQAVSPKQEAQQGRIIERPDGFYWESKETKELRGPFETR
                     GEAEADLLADGAAPEEFDAKDTLQEAESELGISEWIDPDTGGPAEDNVPRIEDH"
     gene            6576..7463
                     /locus_tag="VIG70_09140"
     CDS             6576..7463
                     /locus_tag="VIG70_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8250758.1"
                     /translation="MSQERSALDARAISVMLLLTALWGFQQVTIKLIAADVSLVMQAA
                     VRSIVATVLLLAWARTHRLALFERDGTLVAGLAAGLLFALEFVFIYGGLGYTNASRMS
                     VFVYLAPPLTALGLHFFVAGERLAMLQWTGVLVAFLGLALAFAEGFYSEMNTWTGDLC
                     GFAAALLWAATTVLIRASRLARASATKTLAYQLGVSAVVLLLASWLLGEKGVSAVNGP
                     VLASLAYQALIVAFASYLAWFWLLTRYLAARLSILTFLTPLFGMLFGVVFLSEPLSAY
                     FALAALLIAAGIALVNLRR"
     gene            complement(7383..9182)
                     /locus_tag="VIG70_09145"
     CDS             complement(7383..9182)
                     /locus_tag="VIG70_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEY8250759.1"
                     /translation="METNFLEQALIYLAAGVIAVPLFKKLGLGSVLGYLAAGMAIGPW
                     GLRLIDHPQTVLHFAEFGVVMLLFLIGLELDPKRLWTLRRPIFGMGSVQVVVTAAAVA
                     ALAWALGQPPVVALVAGMGFAMSSTAIALASLQEKNLLSTPGGQASFSVLLFQDLAVI
                     PLLLTVGLLGGKASLDWGAAAIGVGLIAAVITGGRLVLRPALRIIAQTRLREVFVAAA
                     LLLVIGIAVLMESVGLSMALGAFLAGVILADSEYREELEIDIEPFKGLLLGLFFIAIG
                     MSVDLGLFARSPLLVLSLAFGIVLLKVLILFPVANLFGYCGRADAGLFAVALSQVGEF
                     AFVLFAAAGPLLAPETLALLNAAVAVSMLTTPLLFIAYQRIVGRRLAPEKSDVVDEGN
                     PVIVAGFGRFGQVVTRVLNGLRISTTLIDHDPNQVELVRRFGNPAYYGDASRLDVLER
                     AGLARARLLIVAVDDEEAALRMVRQVRRRYPALAIIARAHSRTDGYDYMDLGVPAVRE
                     TFGSALDAAALALRALGHSPEGARRVVSQFRRHDEELMEQMSPHRREVKQLIALNLQG
                     RSDLEKLLTAEIHQRDPGRDEERGEGEVRAERL"
     gene            9198..9737
                     /locus_tag="VIG70_09150"
     CDS             9198..9737
                     /locus_tag="VIG70_09150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY8250760.1"
                     /translation="MDSKPVDAEQFKAGMRALTGAVNIITSTNAGHRYGMTATAVCSA
                     SAEPPTVLVCINKLATTHGAVAKSKAFCVNVLRAEDWELSTTFSGAQSGETRFKSRDW
                     TRLGTGSPVLIDALVSFDCRVVKQMVHGTHTIFLGEVEQVAFGKKGKPLLYSEGQYAK
                     LASLTLGEPLPEGLDYWGF"
     gene            9725..10912
                     /locus_tag="VIG70_09155"
     CDS             9725..10912
                     /locus_tag="VIG70_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8250761.1"
                     /translation="MGFLDNRVKVVLAANFITVAARMSLVTFLGIYFVREAGISLATV
                     GLAFLVENLLRGLAAPFFGALSDRVGRRPLLLAAALVTAAVLPCFLLVSGPGSLFAWS
                     IAMGLASAVKMPVGAALLLDLAPPERRQSVLALNYTVLSVAYTLAVSPAGYIAEQGYA
                     MLAASSAAGYLLVAALYAFGLRGPLPMEVSQREPVMRAALSVSRDRVFLGFAAVAFVF
                     PFSMGLVVTASPLFAAQSGLGEGFIGLVLGLNSIVVALLALPVASRMEGPGPFRMLGA
                     AALLVAACFACYAWIPDVRAALIAGTIVFSFGELVFSSAVPAAVARLAPAGRRGAYQG
                     GWTLVASFSMGSALFVSGLLRDAAGWRPAWTFYAAVTFCAALALLLLRDWFLRRSERP
                     GER"
     gene            complement(10889..11650)
                     /locus_tag="VIG70_09160"
     CDS             complement(10889..11650)
                     /locus_tag="VIG70_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY8250762.1"
                     /translation="MRSSLVTACAGMLVVLGAQAQDGPLTLGAGLHYSSGDYGSTTTT
                     RITTLAATGRYESGAWVYRATVPYLQVKGDTNVIPGVGPVGGTATRSDTASGLGDIVL
                     SATYAAYYDKATTLGMDLTAKLKLATADESKGLGTGEHDVAFLLDLYRTFERITGFGG
                     IGYHVLGDSPSLPLENAWSANLGASYKLDERDSAGAMLEGRQRVIAGGSRQRELVGFF
                     ARKLDRLWKAQAYALIGLADGSPDWGAGLSLARPF"
     gene            complement(11653..12207)
                     /locus_tag="VIG70_09165"
     CDS             complement(11653..12207)
                     /locus_tag="VIG70_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250763.1"
                     /translation="MTKNNLLPLVGAVLLALPLAALPQSQEQLVERYTSLAGSKQNAT
                     SLIAGLRDGKEVTLTGRTGSETFTPPTGKMGYGNVDNALALAEASLKEQGITRPTPLQ
                     LERATMQILGRRADGMGWGEIAKAQGFTLGEVKRADRAAPERAASERVARVERPIKPE
                     RPEKPERPERPERPEKPERPGKGR"
     gene            complement(12302..13675)
                     /locus_tag="VIG70_09170"
     CDS             complement(12302..13675)
                     /locus_tag="VIG70_09170"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY8250764.1"
                     /translation="MEGTRLESPRYVVAFQASPEVGKFFSVEIAACSKPGSPTPENLK
                     VDAHMPEHRHGMNYTPEVKPLGPGRWRAEGLMFHMPGKWEFLFEVRAAGKSDRMGHAL
                     LLSEFSKEEIAKILQHGPWPPPPGRDPSNRVSGKREAIAFGEKLFFEPRLSGTGSVLC
                     ATCHVPFRAFQDGRARAFGLEEVDRNTPSVINTRFYRWYGWDGGHDSLWSQSVRPLLD
                     PREMNASPAHVAGVVRKLFSAEYAAAFGRAVPEENDQILVDVGKALAAFQETLVSGRT
                     AFDEFRDALATNTEASYPAAAQRGLKIFIGQGNCTACHFGPHFTNGEFADTGVPFFAG
                     RGRVDSGRHEGIKRLKADPHNLLGRFNDDQGRATAIGTRHVELQHRNFGEWRVPGLRN
                     VALTAPYMHNGSLATLRDVVKHYSELNEERLHADGERVLKPLKLTESEIDDLVAFLQS
                     LSPRPYK"
     gene            complement(13711..14526)
                     /locus_tag="VIG70_09175"
     CDS             complement(13711..14526)
                     /locus_tag="VIG70_09175"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8250765.1"
                     /translation="MRSFLWAALALPLYAQAQPLPIFDAHVHYSHDAWDVVPVEQAIA
                     IMRKAGVKRALISSSGDDGQQRLFTAAPDLVIPELRVYRTRGETGTWFRDETLIPYLE
                     DRLSRYRYVGIGEFHLYGADADLPVPRQMVQIARKHNLFLHAHSDIDAVERLFKQWPQ
                     ARILWAHAGFERPERVAEMLSKHKNLWADLAFRTDHGSGGKVDPAWRATFLQFPDRFM
                     VGTDTYTPERWHYIPEHAEWSRRWLADLPREVAERIAWKNGETVFGSLLQRPD"
     gene            14583..15242
                     /locus_tag="VIG70_09180"
     CDS             14583..15242
                     /locus_tag="VIG70_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY8250766.1"
                     /translation="MDRILVINPNSTEAVTRGIDEACAPLRMPGGPQIECATLKDGPP
                     GIETQQHVDSVISPLIKKIASTDASAYVIACYSDPGLHTAREATKKPVLGISECGILT
                     ALTLGQKFGVIAILQKSIPRHLRYMGALGVMDRLAGELPVGLPVVELSDEKKTFGRMT
                     EVGKRLRDEHGADVIVMGCAGMARYRKPLQEAVGIPVVEPTQAAVTMAIGRVRLGWES
                     G"
     gene            15239..15784
                     /locus_tag="VIG70_09185"
     CDS             15239..15784
                     /locus_tag="VIG70_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY8250767.1"
                     /translation="MSVAPPTSLRELFLGFLSIGARSFGGVMPWAHRVMVEERRWLNE
                     ADFAETIGLCQFLPGPNIGNASIVLGKRWFGVSGALVAFLGLFALPFIWVLVLFALYA
                     DFAATNATLRAVVTGVGAAGAGLFLGTAVKLGRVIARKPVALALIAACFLAAGIGRVS
                     LFIVLPVSLGVALVCARRGWL"
     gene            15781..16296
                     /locus_tag="VIG70_09190"
     CDS             15781..16296
                     /locus_tag="VIG70_09190"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY8250768.1"
                     /translation="MNLVELTLYFMLLSLISVGGMPSVLPELQRYVVDVKGWITPEEF
                     MQIFAVGQAAPGPNILIASLVGWKVAGLAGALLALFAMCGPSAVLAWWVDDLWVRFKD
                     SAWRKAAQRAIAPIVVALILSGGFILATPGAPDWRLWLIAAATAAGMLATKINPLWFL
                     AGGGIFGAVLL"
     gene            complement(16293..16901)
                     /locus_tag="VIG70_09195"
     CDS             complement(16293..16901)
                     /locus_tag="VIG70_09195"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY8250769.1"
                     /translation="MDLKLAEVSPHERYKLLIGLVIPRPIAWISTRSANGVANCAPFS
                     FFNVFSEEPPLCVIGINPRSDGAMKHSLKNIRRTREFVVNLVDEATANAMHLSSRELP
                     EDESEFETAGLSEAPAALVQHPRIAEAAACLECSLERVIEISGTRQLVLGEILLVHAR
                     DGIIDPQTKRISEERYRPIGRLFADRYCTTRQRFNLPGKLPD"
     gene            complement(16904..17185)
                     /locus_tag="VIG70_09200"
     CDS             complement(16904..17185)
                     /locus_tag="VIG70_09200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY8250770.1"
                     /translation="MFVLTVQLRIKPENVEAFMSKLAENAKNARKEPGCKQFDVLVDP
                     NDKTKAMLYEVYNDDKAFEAHQATPHFKKYVAEAVPLLASRERSVWKRT"
     gene            complement(17206..18141)
                     /locus_tag="VIG70_09205"
     CDS             complement(17206..18141)
                     /locus_tag="VIG70_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HEY8250771.1"
                     /translation="MSRHLKIAAAQLGPLNRSDTRAAAVKRLLALLRESHAMGAKFVV
                     FPELALSTFFPRWWMEDQAEVDARYFEKTMPSKETQPLFDEAKKLGIGFYLGYAELTE
                     KGNRFNSAILVAPDGAIVGKYRKVHLPGHADHKPKAAFQHLEKKYFEVGDLGFRVWRF
                     MGTITGMLICNDRRWPEAFRVLALQGAEIVALGYNTPTENLHYPEPPALRVHHHLIMA
                     QSMAFQNATWLVETAKCGFEDGFRMFGHSLIVAPTGEIAVKSQTEEDEVICANCDIDL
                     AANLKKTMFNFAAHRRPEHYRLIVERVGAEVTPAN"
     gene            complement(18138..19592)
                     /gene="hydA"
                     /locus_tag="VIG70_09210"
     CDS             complement(18138..19592)
                     /gene="hydA"
                     /locus_tag="VIG70_09210"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY8250772.1"
                     /translation="MTTHDLVIRGGTVATAEKTFRADVGISGEKIAALGSNLAGRRVL
                     AAENMLILPGGVDSHCHIEQLSGMGIMAADDFYSATVSAAYGGTTTVIPFCAQHRNDD
                     LKTVLKDYHERARAKAVIDYGFHLIIANPDEPTLSVDLPQAIRSGIRSFKIFMTYDRM
                     RLHDEQILDVMAAARREGALVMVHAENHGMISWLAGRMVKQGNTLPRYHAICHTRASE
                     AEAIERVIKLAELVDCPILIVHVSTPEGIEAIRSARKNGLKVYGETCPQYLFLTAKDI
                     DIGLQGAMFCCSPPPRDEKAQEACWRGLKDGALHVYSSDHAPYRMDASGKLPKGDKTT
                     FKEMANGVPGLELRLPLLFTFGVKAGRISLEEFVNLTSARHAQTYGLYPRKGTIAVGS
                     DADIAVWDPDRELVIEKTRDNTGYTPYKGRKLTGWPVTVLSRGEIIIENGKLSAERGR
                     GRFLAREPSEALKPLGREVPEISQLKAWNTPLKL"
     gene            complement(19608..20303)
                     /locus_tag="VIG70_09215"
     CDS             complement(19608..20303)
                     /locus_tag="VIG70_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8250773.1"
                     /translation="MAGKTAVVTGAASGIGAAIVERFLAEGAKVLAVDLNPIKPRPGL
                     QTLVADVSRPAPKLPPADVLVCAAGISVGKALADTSEEDWDRVFAVNAKGTFLWFKAV
                     LPGMLERGGGSLIAIASQLAVAGPRSNCSYAASKGAVVSLVKSVALDYADRGIRANAL
                     LPGATETPLLERAFARRADPKAARETLRQRHPAGRFGRPEEIAAAALFLASDESSFVT
                     GAALPVDGGFLAG"
     gene            complement(20293..>21012)
                     /locus_tag="VIG70_09220"
     CDS             complement(20293..>21012)
                     /locus_tag="VIG70_09220"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335276.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY8250774.1"
                     /translation="GAVVFDNGNLIRTQAQKGGVADAFDIPIFSEAFLRPLFSRGIGP
                     FRWIALGGTPEDIARIDDLVLELFPENRVAANWIRLARAQVPFQGLPARIAWLGHGER
                     TRLGLAVNDLVRKKVLSAPIAFTRDHLDAGAMAHPNIMTERMLDGSDAIADWPLLNAM
                     VNCASMADLVAIHSGGGGYAGYMTSAGVTLIADGTPEADRRLELSLTNDTATGVMRYA
                     DAGYEEARDEARRKSIHVFGG"
BASE COUNT         3220 a         7032 c         7214 g         3546 t
ORIGIN      
        1 ggatctcgtc ggcgccccga tcgccgctgc caagctctcc caggccaccg cctcggcggt
       61 cgcggcctgc gatgcggccg acggtgtggt cgacggcgtg atcgacgatc cgcggacgtg
      121 caagttcagc gcgacggcca acgtctgcgg caccgcgacc gctccggcgg cgaactgcct
      181 gacgctccag gaggccgatg ccatcgacct gatctgggac gggccgcgga acgcgcgcgg
      241 tagcaagata tggttcgggc tcgatcgcgg aaccaacctg agcggcctga acggcaccaa
      301 cccgttcgcg ctcggcgtga cccagttcca ctgggacatg cacgaccgca acttcgactg
      361 gcgcaccgtg acgatggcga cgtacgcgga cgtcgcgcag gccggctcgc gcaatatcgc
      421 cgacgtcacc gacaccttcg gcgatctcga caccttccgt cgcagcggcg gcaagctgct
      481 caccttcgtc ggcgcgaacg accagctcat catgccgcgc ggggtgatca actactaccg
      541 cgagatggcg gcgcgcgatg gcaagcacga cgagccggat ttcgaccggt tgcagcgctt
      601 ctaccgcctg ttccgcgctc cgggagtcgc ccactgtggc ggcggtgccg gaccacagcc
      661 gcagaacctg ttcggcgcgc tggtcgactg ggtggagaag ggcatcgcgc cggagcagat
      721 cctcgggctg cagaacctcg gcggcggcct cacgcgcacg cggccgattt gcctgtatcc
      781 gcagaccgcg atctacaacg gcaccggcag caccgacgac gcggccaatt tccactgcgg
      841 cggaaacctc gagaggcggc gcaccgtttg cgccgacgcg ctcgcgaaat acaagcacga
      901 agtgaacggc cgcctggact tcgacggcac cggcctcgac cgggaagatt gcgacggccg
      961 gcgccatcac cacgaccact ggcactggta gccggtccga ttgacctaga cgccccggca
     1021 aaccggggcg tcttgctttc gggcttgcgg gttagaatcc cccttcgcag cggagagatg
     1081 cccgagtggc cgaagggggc gccctgctaa ggcgttatac cggcttaaac cggtatcgag
     1141 ggttcgaatc cctctctctc cgccaatctc cttcccattt gccatgtccc tcggccgcgt
     1201 cctcgtagag aaattccagc ccgacgtgct cgaatggctg cgcgcacggg ccgaggtcgt
     1261 cgtcgtcgac ccgtgggccg agcccgatcg gtgggaagcc gaagcgccgg cggtcgacgc
     1321 ggtcatcagc cgcaaaggca ggatcacccg gcagcacatg gagaagagcc acggccgcct
     1381 gaagatcgtc gcgcgcaccg gggtagggtt cgatgcctcg cggatcgatg tcgacgcggc
     1441 gaaggagcac aaggtgtggg tgaccaacat gcccggaagc aacgcgatcg cggtcgcaga
     1501 gcttgcgatg ggccagctgg tcgcgctcgc gcgccacacc atggcggcga accgcgcggt
     1561 caaggaaaac cgctggggcg gcgactatct gcggctcatc ggcgtcgagc tcgccggaaa
     1621 gacgctcggc atcgtcggca tgggaaacat cggcacgcgg gtcgcgctgc gggcgcgcgc
     1681 gttcgagatg tcgctcctcg tctgcgatcc gtacattccc gcctcgcatg tgacctccct
     1741 gggaggccgc tgggccggtc tcgacgaggt atttggcgaa gcggacttcg tcacgctgca
     1801 ctgcccgctc aatcgcgaga cccggcacct ggtgggcgag cggcagctct cgcgcatgaa
     1861 gcgctcggcc tatctcgtca acctcgcccg cggcggcgtg gtcgacgaag aagccttgta
     1921 caagtgtctt agcgaaaaac gcatcgccgg tgcggccatc gacgtcctgg agaccgagcc
     1981 gccgcgcagg gatcatccgc tgatcgggct cgacaacgtg atcttctcgc cccatatcgg
     2041 cggctcggcg cgcgaggcga cgagccgcgg cgagcgcggc gcggccgagg aggtcgtgcg
     2101 cgtcctcgag ggcaagagtc ccctcaatcc agtctttcaa aaatagccct ttcagcgggt
     2161 acggccggga accaaatttg ctcggccttg cccgatatgc caatggccaa ggacctctac
     2221 agggtaggga tcaccggatc gtacggcggg ctaaacctcg gcgacgaggc gatcctgcag
     2281 tcgatcatcg cccagctgcg caaggacctg ccgaagctcg agatcacggt gttctcgcgc
     2341 gacgcggatg acacgaagcg ccgccaccag gtcgagcgcg ccgtgccggt gcgcaagctc
     2401 tcgcgcgccg agatcatgcc cgaggtcgag cgcctcgatc tcctgatcct gggcggcggc
     2461 ggaatcctgt acgacgccga cgcccgcacc tacctgcgcg aggtgcagct cgcgcgcgag
     2521 aagcgcgtgc cggtgttcgt gtatgcgatc ggcgccgggc cgctgaccca cgccgcggcc
     2581 caggccgcgg tgcgcgagaa cctcgccgac gtcgagatgg tgacggtgcg cgagaaaagc
     2641 gcgcaccgcg tgctcgagga gtcgggcatg caccgcgacg acgtggtcgt gaccgccgat
     2701 ccggcgctgc tgctcaagcc cgagccgctg ccgcgcggca tcctcaggca cgaggggctg
     2761 gaaggccgcc ggcggctgat cggcatgtcg gtacgcgagc ccggcgtcgc ggcgcccgac
     2821 ctcgacgaga aggtgtacca cgcgctgctc gccaacgccg cggacttcat ggtggaccgc
     2881 tggaacgcga acgtcgtgtt cgtgccgatg gagcgctcgg tgctcgacac gcagcacagc
     2941 cacgcggtga tcgcgaagat gctgcgcgcg cagcgcgcca ccgtgctcaa gggcgaatac
     3001 acggccgggc aggttctttc ctggatgggc aagttcgact tcgcgctcgg catgcggctg
     3061 cacttcctca tcttcgccgc catccagggc acgccgttcg ttgcgctgcc gtacgccgga
     3121 aaggtgagcg gcttcctcga agcgctcggc gtgcccaccc cgccgctcaa cctggtgaac
     3181 gcgggacggc tcatcgccta tctcgacgac tcgtgggacc ggcgccgctc gatgcgcacg
     3241 cgcatcgccg agtcgctgcc cgcgctgcag gagcgcgcgc gcgaaaccca ccgcctgctg
     3301 ctcgaggtgc tgaccggaga aaaatcgcgg aatgcccaca ttaaagcggc ctgacgacgc
     3361 gccgagcatc gagctgccgc cccgtcaggc gctgcttgcg ggcgaggccg acgtgctggt
     3421 cgtcggcggc ggcccggcgg gccttggcgc tgcgctcggc gcggtccagg ccggcgcgcg
     3481 cgtgatcctc gccgagcgct acggcttcct cggcggcaac gccaccgcgg cgctggtgat
     3541 gccgctcatg tcgttccaca cgcagacgcc aacgccggag cggcgcggcg ccaccacgct
     3601 gctgcccacc gatcacggcc cgggcgaagc ggtggttggc ggcgtcctgg cgaagctgct
     3661 ggcgcgactg gtcagcgtgg gcggggcgat cccgcccacc ctcgcgaccg gctatgtcgt
     3721 gccgttcgac cccgagtggt tcaagatcgt cgcgctcgac ctgctggacg aggccggcgt
     3781 gcagatcctg ttccacgctt tcgcgagcgg cgtgctgggc gaaaaccggg tcgaaggcgt
     3841 ggtgttcgag accaagtccg ggccgctcgc gatccgcgcc aaggtcacga tcgattgcac
     3901 cggcgacgcc gatctcgcgg tgcaggcggg tacgcccacc gaaaccggcc gcgccgacgg
     3961 actggtgcag ccgatgacgc tgatgttccg catggccgag ttccaccgcg ccgcgttcga
     4021 aggctacgtc aaggagaacc cgaagcagtg gcgcggcgtg cacggcctgt gggacctggt
     4081 gcgggaggcg acggcggcgg gcgcgctcaa gctgccgcgc gaggacatcc tgttcttcgc
     4141 cacgccgcac gaaggcgagg tgagcgtcaa cagcacgcgc gtcacgcgcg tgctcggcac
     4201 cgacgtctgg gacctctcct acgccgaatg gatgagccgg cgccagatgc gccagatcgc
     4261 ggaattcctc aagcaatacg tgcccgggtt cgagaagtcc tacgtgatgc aaagcggagt
     4321 gaacgtcggc gtccgcgaga cgcggcgcat cattggcgac taccagctca ccgtcgacga
     4381 cgtgctctcg gcgcgcaagt tcgacgatgg catcgcgcgc ggcgcgtatc ccgtcgacat
     4441 ccacaatccc gccgggacag gcactgtgct caagcgcctg ccgccgggcg aggcctacga
     4501 cattccgctg cgctgcctga tgccgaagaa tgccgaggga ctggtcgtcg ccggccgctg
     4561 catctcgggc acgcacgagg cgcactcgtc gtatcgcgtg atgcccatcg tgatggcgac
     4621 cggacaggcc gccgggatca ccgccgcgat cgcggcccgc cgcggcgccc gcccgcgtga
     4681 aatatctgtc aaagaagttc aatacgaaat cgtccgccag ggcggtagcc tgcgaaaaga
     4741 actgcatccc gcaaaagcag cgtgatgtag ttgaaagagg gatatggcgc tccgcagagc
     4801 gccattagca ttaccgccaa tgactaggga gagcgagctc cgcgtcgtca ccgacgccat
     4861 gcccgcggca gcggtgcgct gcgcgcggga ccagaccttc ctctgggtga acccccgcta
     4921 cgcaagctgg atggcgcgcc ccgccgacgc gatcatcggc cgcaagctgg cggaggtcat
     4981 cggccgggaa gcgatgtaca ccatcgagcc gttcatccag cgggtgctga agggcgagca
     5041 ggtgcagtac gagcggctgg cgcgcttcgc gggcctcggt gagcgctggg tgggctggat
     5101 gtacacgccc accccggatg ggtgggtggc gatcggcacc gacatccacg accgcaagct
     5161 cgccgaggag gcgctgaagg ccgagcacca gcgcaaggac gagttcctcg ccgcgctcgc
     5221 ccaccagctg cgcaatccgc ttgccccgat ccgcaacgcg gtcgcgatcc tggcgcgcaa
     5281 gggctcgcag gatcccgaag tggcgtggag ccaggccgtg atcgagcgcc agatcgagca
     5341 gctctcgcgc ctgatcggcg acctggtgga cgtcgagcgc atcgcccgcg gcaacctcgc
     5401 gctgcgccgc gagcgcgtga cgctggaggc ggtcgtcgac atggcgctcg agtcgagcag
     5461 accgcaagtg aacgcaggcg gtcaccggct ctcggtgctc ctgccgagcg agcgggcgat
     5521 gctcgatgcc gaccccgcgc gcctggcgca ggcgctcgcg acgctgctca agaacgccgc
     5581 caagcacacg cccccgcagg gcacgattac cctaagtgcc gcggtcgagg gcggcgaggt
     5641 ggtggtcgcg gtcgaggaca gcggcgtcgg cttcgatgcg cctgccgccc gcagcctgtt
     5701 cgtcaaagaa gccggcgtcg gcttgcgact cgtgcacggc atcgtcgcgc tgcaccgcgg
     5761 gcgcattgaa gcgcgcagcg ccgggccggg tcgcggcgcg gagttcgtcg tgcgccttcc
     5821 gcgcgcgagc ggggtctcaa ggcccgcaac gacgtctcct cattacaccg gctacagcgc
     5881 cgcggcaccg gtgcgcgtgc tcgtcgccga cgacaatcgc gatgcggccg actcgctcca
     5941 gcgtgtgctc gcgctctacg gtcatgaagt gcgcgtcgcc tacgacggcg ctaccgcgct
     6001 caggatcggg caggaattca ggccgcgggt ggcggtgctg gacatcgcga tgcccggcag
     6061 cgacggctac caggtggcgc aggcgattcg caggcagcag ggcagggagg tgacgctcgt
     6121 cgccctgacc ggctggggcc aggaagccga ccgcaggcgc gcgatggagt cgggcttcga
     6181 tcaccacctg gtgaagccgg tcgacccgaa cgccctcaac gccctccttg cggaggtcat
     6241 tgagaagtag ctagtggtcc tcgatgcggg ggacgttgtc ctccgccggc ccgccagtgt
     6301 cggggtcgat ccactccgag atgccgagct cggattcggc ttcctgcagg gtgtccttgg
     6361 cgtcgaactc ctccggagcg gcgccgtcgg cgagcaggtc ggcctcggcc tccccgcggg
     6421 tttcgaacgg gccgcgcagc tccttcgttt ccttcgactc ccagtagaag ccgtcgggcc
     6481 gctcgatgat ccggccttgc tgtgcttctt gcttgggtga aacagcctgt ttcttccgcc
     6541 ccggcatcag ccgatgttac gccaaaatcg gcgcgatgag tcaagagcga agcgcgctgg
     6601 acgcacgcgc gatttccgtg atgctgctgt tgaccgcgct gtggggtttt cagcaggtca
     6661 cgatcaagct gatcgctgcc gacgtgtcgc tcgtcatgca ggccgcggtc cggtcgatcg
     6721 tcgccacggt gctgctgctc gcttgggcgc gaacccatcg tctcgcgctc ttcgagcgcg
     6781 acggcacgct cgtcgccggc ctcgccgcag ggctgctgtt cgccctcgag ttcgtattca
     6841 tctacggggg cctcgggtac accaatgcct cgcgcatgtc ggtcttcgtg tacctggcgc
     6901 cgccccttac cgcgctcggc cttcacttct tcgtcgccgg cgagcgactc gcgatgctgc
     6961 agtggacggg ggtgctggtt gcctttctgg gcctcgcgct cgccttcgcg gaaggcttct
     7021 actcggagat gaatacatgg accggggatc tgtgcggttt cgcggccgcg ctgctctggg
     7081 ccgcgaccac ggtgctgatc cgcgccagcc gcctcgcgcg cgccagcgcg actaagacgc
     7141 tcgcctatca gctcggggtt tccgccgtcg tgcttctcct tgcctcctgg ctcctcggcg
     7201 agaaaggcgt gagcgcggtg aacggcccgg tgctcgcgag ccttgcctac caggccctga
     7261 tcgtggcatt cgccagctac ctggcgtggt tctggctgct gacgcgctac ctggcggcgc
     7321 ggctctccat cctcacgttc ctcacgccgc tcttcggcat gctctttggc gtggtgtttc
     7381 tctcagagcc gctcagcgcg tacttcgccc tcgccgcgct cctcatcgcg gccgggatcg
     7441 cgctggtgaa tctccgccgt taggagcttc tcgaggtcgc ttctgccctg caggttgagc
     7501 gcgatgagct gcttcacctc gcggcggtgc ggcgacatct gctccatcag ctcctcgtcg
     7561 tggcgccgga actggctgac gacgcggcgc gcgccctcgg ggctgtggcc gagtgcgcgc
     7621 agcgcgaggg cggcggcgtc gagcgccgag ccgaaggtct cgcgcaccgc gggcacgccg
     7681 aggtccatgt agtcgtagcc gtcggtgcgg ctgtgcgcgc gggcgatgat cgcgagggcg
     7741 ggatagcgcc tgcggacctg ccgcaccatg cgcagcgctg cctcttcgtc gtcgacggcg
     7801 acgatcagca gccgggcgcg ggcgaggccc gcgcgctcga gcacgtcgag ccgcgaggcg
     7861 tcgccgtagt aggccgggtt gccgaacctg cgcaccagct cgacctggtt ggggtcgtgg
     7921 tcgataaggg tggtgctgat gcgcaagccg ttcagcacgc gcgtcacgac ctggccgaag
     7981 cggccgaagc ccgccacgat gaccgggttg ccttcgtcga cgacgtcgga cttttccggg
     8041 gcgagccgcc ggcccacgat gcgctggtac gcgatgaaaa ggagcggcgt ggtcagcatc
     8101 gacactgcga ccgccgcgtt cagcagcgcg agcgtttcag gggccagcag cggacctgcg
     8161 gcggcgaaca gcacgaacgc gaactcgccg acttgcgaca gcgcgacggc gaacagtccc
     8221 gcgtcggcgc gcccgcaata gccgaagagg ttcgccaccg gaaagaggat caggaccttg
     8281 agcaggacga ttccgaaagc gaggctcagc acgagcagag gcgagcgcgc gaacaggccg
     8341 agatccacgc tcatgccgat ggcgatgaag aacaggccaa gcaagagccc cttgaacggc
     8401 tcgatgtcga tctcgagctc ctcgcggtat tcgctgtcgg cgaggattac cccggcgagg
     8461 aaagcgccga gcgccatcga caggccgacc gattccatca gcacggcgat gccgatcacc
     8521 agcaggagtg ccgcggcgac gaatacttcg cgcaatctcg tctgggcgat gatgcgcagc
     8581 gccgggcgca ggacgaggcg cccgccggtg atcacggcgg cgatgaggcc gacaccgatg
     8641 gcggccgcac cccagtcgag cgacgccttg ccgccgagca gcccgacggt gaggagcagc
     8701 ggaatcaccg ccaggtcctg gaacagcagc accgagaacg acgcctgtcc gcccggcgtc
     8761 gagagcaggt tcttctcctg cagcgatgcg agcgcgatcg cggtcgagga catcgcgaat
     8821 cccattcccg cgaccaacgc caccaccggc ggctgtccca gcgcccaggc gagcgccgcg
     8881 accgccgccg cggtcacgac cacctgcacg cttcccatgc cgaagatcgg ccggcgcagc
     8941 gtccacaggc gtttcgggtc gagctcgagg ccgatgagga acagcagcat cacgacgccg
     9001 aactcggcga aatgcagcac cgtctgcgga tggtcgatga ggcgcagtcc ccacggaccg
     9061 atcgccatgc ccgcggcgag atagccgagc accgatccga gcccgagctt cttgaaaagc
     9121 ggaaccgcga tcacacccgc ggcgaggtag atcagggctt gctctaggaa gtttgtctcc
     9181 atggttagaa tgctctcatg gatagcaaac cggtcgacgc tgaacaattc aaagccggaa
     9241 tgcgcgcgct caccggcgcg gtgaacatca tcaccagcac gaacgcaggc caccgctacg
     9301 gcatgaccgc gaccgcggtg tgctcggcga gtgccgagcc gccgacggtg ctggtgtgca
     9361 tcaacaagct cgccaccact cacggcgcgg tggcgaagag caaggcgttc tgcgtcaacg
     9421 tgctgcgcgc cgaggactgg gagctctcga ccaccttcag cggcgcgcag agcggcgaga
     9481 cccgcttcaa gtcgcgcgac tggacgcgcc tgggcaccgg ctcgccggtg ctgatcgacg
     9541 cgctggtgtc cttcgactgc cgcgtggtga agcagatggt ccacggcacc cacaccatct
     9601 tcctcggcga ggtcgagcag gtggccttcg gcaagaaggg caagccgctg ctctactcgg
     9661 aaggccagta cgcgaagctc gcttctctca cgctgggcga gcccctgccc gagggcctcg
     9721 actactgggg tttctagaca acagagtcaa ggttgttcta gcggcaaact tcatcaccgt
     9781 cgccgcgcgg atgagcctgg tgacgttcct cggcatctat ttcgtgcgcg aggcggggat
     9841 cagcctcgcg accgtcggcc tcgcgttcct ggtggagaac ctgctgcgcg gcctcgcggc
     9901 gccgttcttc ggcgcgctga gcgaccgcgt cggccgccgg ccgctgctgc tcgcggccgc
     9961 gctcgtcacc gcggccgtgc tgccgtgctt cctcctcgtg agcggtccgg ggtcgctctt
    10021 tgcgtggagc atcgccatgg ggctcgccag cgcggtcaag atgccggtcg gcgcggcgct
    10081 gctcctcgat ctggcgccgc cggaacgccg gcagtcggtg ctggcgctca attacaccgt
    10141 gctcagcgtc gcctacacgc tggcggttag cccggccggt tacatcgccg agcagggtta
    10201 cgcaatgctc gcggcgtcga gcgccgcggg ttacctgctc gtggccgcgc tctacgcgtt
    10261 cggcctgcgc gggccgttgc caatggaggt ttctcaaagg gaaccggtga tgcgcgcggc
    10321 gctctccgtt tcccgggacc gcgtgttcct gggcttcgcc gcggtggcgt tcgtctttcc
    10381 cttcagcatg gggctggtcg tgaccgcgtc gccgctgttc gccgcgcaaa gcggcctggg
    10441 cgaaggcttc atcggcctgg tgctcgggct gaacagcatc gtcgtcgcgc tgctcgcgct
    10501 gcccgtcgct tcgcgcatgg aggggccggg accgttccgc atgctcggcg cagccgcgct
    10561 gctggtcgcc gcctgcttcg cctgctacgc gtggattccc gacgttcgcg ccgcgctgat
    10621 cgccggcacc atcgtgttca gcttcggcga gctggtgttc agctccgcag tgccggcggc
    10681 ggtggcgcgc ctcgcgccgg ccggccgccg cggcgcctac cagggcggct ggaccctggt
    10741 tgcgtcgttc agcatgggaa gcgcgctttt cgtcagcggc ctgctgcgtg acgccgccgg
    10801 ctggcgcccg gcctggacct tctatgccgc cgtcactttc tgcgcagctc tcgcgctgct
    10861 gcttctgcga gactggttcc tcaggcggtc agaacggccg ggcgagcgat aggcccgcgc
    10921 cccagtccgg gctgccgtcg gcgaggccga tgagggcgta ggcctgcgct ttccagaggc
    10981 gatcgagctt gcgcgcgaag aagccgacga gctcgcgctg ccgcgagccg cccgcgatca
    11041 cgcgctgccg gccttccagc atcgcaccag cgctgtcgcg ctcatcgagc ttgtagctcg
    11101 cgccgagatt cgcgctccag gcgttttcga gcggcagcga gggtgagtcg ccgaggacgt
    11161 gatagccgat cccaccgaag ccggtgatcc gctcgaacgt gcggtagaga tcgagcagga
    11221 aagccacgtc gtgctcgccg gtgccgaggc ccttgctctc gtcggcggtg gcgagcttga
    11281 gcttcgcagt gagatccatg ccgagcgtcg tcgccttgtc gtagtacgcg gcataggttg
    11341 ccgagagcac gatgtcaccg agccccgagg ccgtatccga tcgggtcgct gtcccgccga
    11401 ccggccctac gccaggaatc acgttcgtgt cgcccttgac ctgaaggtag ggcacagtcg
    11461 cccggtacac ccatgcgccg ctctcgtagc ggccagtcgc ggcgagcgtc gtgatgcggg
    11521 tcgtggtcgt gctgccgtag tcgcccgagc tgtagtgcag cccggcgccc agcgtaaggg
    11581 gaccatcctg cgcctgcgct ccaaggacga cgagcatccc ggcgcaagcc gtgacgaggg
    11641 aactgcgcaa ggttagcggc ccttgccggg tcgctcaggt ttttccggac gctccggccg
    11701 ctccggccgt tcgggcttct cgggacgctc gggcttgatc gggcgctcaa ccctggccac
    11761 gcgctccgaa gcagcgcgtt ccggcgccgc gcggtccgca cgcttgactt cgccgagcgt
    11821 gaagccttgc gccttcgcga tttcacccca gcccatgccg tcggcgcgcc gcccgaggat
    11881 ctgcatcgtc gcgcgctcga gctggagggg cgtcggccgg gtaatgcctt gctccttcag
    11941 cgacgcttcg gcgagcgcga gcgcgttgtc gacgttgccg taacccatct tgccggtcgg
    12001 cggcgtgaag gtctcgctgc cggtcctgcc ggtcagcgtg acttccttgc cgtcgcgcag
    12061 gccggcgatc agcgaagtcg cgttctgctt ggacccggca agcgaggtgt agcgctcgac
    12121 tagctgctct tggctctgcg gcaacgctgc gaggggtaag gcgagcagca ctgccccgac
    12181 cagcggcagc aggttgttct tggtcatgga tagctccgaa gactaagaga accagcatgg
    12241 tagccagcac gcgccgcgac ctgaagcgga atcggtcacg acgccggatg gtccaacagg
    12301 actacttgta gggccgcggc gacaacgact gaaggaacgc aaccaagtcg tcgatctcgc
    12361 tctcggtcag cttgagcggc ttcagcacgc gctcgccgtc ggcatgcagc cgctcctcgt
    12421 tcagttccga gtaatgcttc actacgtccc tcagcgtcgc caggctgccg ttatgcatat
    12481 agggagcagt gagcgcgacg tttcgcagcc cggggacccg ccattcgccg aagttgcggt
    12541 gctgcagctc gacatggcgc gtaccgattg ccgttgcacg accctgatcg tcattgaagc
    12601 gcccaaggag gttgtgcgga tcggctttca gccgtttgat tccttcgtgc cgcccggaat
    12661 cgacccggcc gcgtcccgca aagaacggca cgccggtatc ggcgaactcg ccgttggtga
    12721 agtgcggccc gaagtggcag gcggtgcagt ttccctggcc gatgaaaatc ttcagcccgc
    12781 gctgcgccgc cgcgggatag ctcgcttcgg tgttcgttgc gagcgcatcc ctgaattcgt
    12841 cgaaggccgt gcgcccgctg accagcgtct cctggaacgc cgccagcgcc ttgccgacgt
    12901 cgacgaggat ctggtcgttt tcctcgggga cggcacggcc gaacgcggct gcgtactcag
    12961 cggaaaaaag ctttctgacc acgcccgcga catgcgccgg actcgcgttc atctcccggg
    13021 ggtcgaggag cggccgcacg ctctgcgacc acaggctgtc gtgcccgccg tcccatccgt
    13081 accagcggta gaagcgcgtg ttgatcacgc tcggggtatt gcgatcgact tcctcgagcc
    13141 cgaaggcgcg cgcgcggccg tcctggaagg cgcgaaaggg cacgtggcag gtcgcgcaca
    13201 gcaccgagcc ggtgccggag agccgcggct cgaagaagag cttctcgccg aacgcgatcg
    13261 cctctcgctt ccccgagacg cgattgctcg ggtcgcggcc cggcggcggc ggccaggggc
    13321 cgtgctgcag gatcttcgca atttcttcct tcgagaattc cgaaagcaac agcgcgtggc
    13381 ccatccgatc gctcttcccg gcggcgcgaa cctcgaatag gaactcccat ttgcccggca
    13441 tgtgaaacat caacccctcg gcgcgccagc gtcccggccc gagtggcttc acttccggcg
    13501 tgtagttcat gccgtggcga tgctccggca tgtgcgcatc gactttcagg ttttccggag
    13561 ttggactccc gggcttggag cacgccgcga tctcgacgga aaagaatttt ccgacctcgg
    13621 gagaggcttg aaaggcgacg acgtaccgcg gcgactcgag ccgcgtaccc tccaactcag
    13681 gaacgcacgc atgcgccccg cagaggaaac tcagtccggc cgctgcagca gcgagccgaa
    13741 gacggtttcg ccgtttttcc atgcgatgcg ctcggcgacc tcgcgcggca ggtccgcgag
    13801 ccagcgccga gaccactcgg cgtgctcggg gatgtagtgc cagcgctcgg gagtgtaggt
    13861 gtcggtgccg accatgaagc ggtcggggaa ctggaggaag gtcgcgcgcc acgccgggtc
    13921 gaccttgccg cccgagccgt ggtcggtgcg gaacgcgagg tcggcccata ggttcttgtg
    13981 cttgctcagc atctcggcga cgcgctcggg gcgctcgaag cccgcgtgcg cccaaagaat
    14041 gcgcgcttgc ggccattgct tgaacaggcg ctcgaccgcg tcgatgtcgg agtgcgcatg
    14101 caggaagagg ttgtgctttc gcgcaatctg caccatttgc cgcggtaccg gcaggtcggc
    14161 gtcggcgccg tagaggtgga actcgccgat gcccacgtac cggtatctgc tcagcctgtc
    14221 ctcgagatac gggatcaggg tctcgtccct gaaccaggtg ccggtctcgc cgcgcgtgcg
    14281 gtagacgcgc agctccggaa tcaccaggtc gggcgcagca gtgaacaggc gctgctggcc
    14341 gtcgtcgccc gagctcgaga tcagcgcgcg cttgacgccc gccttgcgca tgatggcgat
    14401 cgcctgctcg accggcacca cgtcccacgc gtcgtggctg tagtgcacgt gcgcgtcgaa
    14461 gatcggcaac ggttgtgcct gagcatagag gggcagcgcc aaggctgccc agagaaagct
    14521 gcgcatggtc gaagtgtaga atgccccgca catcacgtca cgcggaaaga cgtactgcac
    14581 tgatggacag gattctcgtc atcaacccca actcgaccga agcagtgacg cgcggcatcg
    14641 acgaagcctg cgcccctttg cgcatgcccg gcggcccgca gatcgaatgc gccacgctga
    14701 aagacggacc gccggggatc gagacgcagc agcacgtgga ctccgtgatt tcgccgttga
    14761 taaaaaagat agcttccacg gatgcgagcg cgtacgtcat cgcctgctat agcgaccccg
    14821 ggctgcacac ggcgcgcgag gcgaccaaga agccggtgct gggcatttcc gagtgcggca
    14881 tcctgaccgc gctgacgctc ggccagaagt tcggtgtcat cgccatcctg cagaagtcga
    14941 ttccgcggca ccttcgctat atgggcgccc tcggcgtgat ggaccggctg gccggagagc
    15001 tgccggtcgg attgccggtg gtcgagcttt cggatgaaaa gaaaactttt ggccgcatga
    15061 cggaggtcgg caagcggctg cgcgacgagc acggcgccga cgtcatcgtg atgggctgcg
    15121 ccggcatggc gcgctaccgc aagccgctgc aggaagcggt gggcattccc gtggtggagc
    15181 ccacccaggc ggccgtgacc atggcgatcg ggcgggtgcg gctggggtgg gaaagcggtt
    15241 gagcgtcgcg cctcccactt cgctgcgcga gcttttcctc ggctttcttt ccatcggcgc
    15301 ccgctctttc ggcggcgtca tgccgtgggc gcaccgcgtc atggtggaag agcggcgctg
    15361 gctcaacgag gccgatttcg ccgagaccat cggcctgtgc cagttcctgc cggggccgaa
    15421 catcggcaac gcctcgatcg ttctgggcaa gcgctggttc ggcgtgtcgg gcgcgctggt
    15481 cgcgttcctc ggcctgttcg cgcttccctt catttgggtg ctggtcctgt tcgcgctgta
    15541 cgccgacttc gccgccacca atgcgacgct gcgtgcggtg gtcaccggcg tcggcgccgc
    15601 gggcgcgggc ctcttcctcg gcactgcggt caagctcggc cgcgtgatcg cgcgcaagcc
    15661 ggtcgcgctc gcgctgatag cggcgtgctt cctcgcggcc gggatcggcc gggtatcgct
    15721 cttcatcgtt ctgccggtat cgctcggcgt ggcgctggtc tgcgcgcgca ggggctggct
    15781 gtgaatctcg tcgagctcac gctctacttc atgctgctgt cgctcatctc ggtgggcggc
    15841 atgccctccg tccttccgga gctgcagcgc tacgtggtcg acgtcaaagg ctggatcacg
    15901 ccggaagaat tcatgcagat attcgccgtc ggccaggccg cgccggggcc gaacatcctt
    15961 atcgcgagcc tggtgggctg gaaggtcgcg gggctcgcgg gtgcgctgct cgcgcttttc
    16021 gcgatgtgcg gcccgtccgc ggtgctcgcc tggtgggtcg acgacctgtg ggtgcgcttc
    16081 aaggattcgg cgtggcgcaa ggcggcgcaa cgcgcgatcg cgccgatcgt ggtggcgctg
    16141 attctctctg gcggcttcat cctcgccacg cccggcgcgc ccgactggcg gctgtggctg
    16201 atcgccgccg cgaccgccgc cgggatgctc gcgacgaaga tcaatccgtt gtggttcctc
    16261 gccgggggtg ggattttcgg agcagttctg ctttagtcgg gcagctttcc gggaaggttg
    16321 aaccgctgtc gcgtggtgca gtaacggtcg gcgaagagcc gcccgatcgg gcggtagcgt
    16381 tcctccgaga tgcgtttggt ctgcgggtcg atgatgccgt cgcgcgcgtg caccagcagg
    16441 atctccccga gcacgagctg gcgcgtgccg ctgatctcga tcacgcgctc gagactgcac
    16501 tcgaggcacg cggccgcctc ggcgatgcgc gggtgctgga ccagcgcggc cggcgcctcg
    16561 ctaaggccgg ccgtctcgaa ctcgctctcg tcctcgggga gctcgcggct cgagaggtgc
    16621 atcgcgttgg cggtcgcctc gtcgaccagg ttgacgacga actcgcgcgt gcggcggatg
    16681 ttcttgagcg aatgcttcat cgcgccgtcg ctgcgcgggt tgatgccgat cacgcagagc
    16741 ggcggctcct cggagaacac gttgaagaac gagaacggcg cgcagttggc gacgccgttc
    16801 gcgctgcgcg tcgagatcca ggcgatcggc cgcgggatca ccaggccgat gaggagcttg
    16861 tagcgctcgt gcggcgagac ctccgcgagc ttgaggtcca ctcctaggtg cgcttccaga
    16921 ccgagcgctc gcgcgaggcg aggagcggca ccgcctcggc gacgtacttc ttgaagtgcg
    16981 gcgtcgcctg gtgcgcctcg aaggccttgt cgtcgttgta gacctcgtac agcatggcct
    17041 tggtcttgtc gttcggatcg accaggacgt cgaactgctt gcagccgggc tccttgcgcg
    17101 cgttctttgc gttttcggcg agcttgctca tgaacgcttc gacgttctcc ggcttgatcc
    17161 tgagctgtac ggtcagcacg aacatggggt ctccgtggcg ttttgctagt tagcgggggt
    17221 gacttccgcg ccgacgcgtt cgacgatcag gcggtagtgc tcggggcggc ggtgcgcggc
    17281 gaagttgaac atcgtcttct tcaggttggc ggcgaggtcg atgtcgcagt tggcacaaat
    17341 cacctcgtct tcctcggtct ggctcttcac cgcgatctcg ccggtgggcg ccacgatcag
    17401 cgaatggccg aacatgcgga atccgtcctc gaagccgcac ttcgcggtct cgaccagcca
    17461 ggtggcgttc tggaacgcca tcgactgcgc catgatgagg tggtggtgca cgcgcagggc
    17521 gggcggctcg ggatagtgca ggttctcggt cggcgtgttg tagccgagcg cgacgatctc
    17581 ggcgccctgc agcgcgagca cgcggaacgc ctcgggccag cgccggtcgt tgcagatcag
    17641 catgccggtg atcgttccca tgaagcgcca cacgcgaaag ccgagatcgc cgacctcgaa
    17701 gtacttcttc tccaggtgct ggaatgccgc cttgggcttg tggtcggcat gcccgggcag
    17761 gtgcaccttg cggtacttgc cgacgatcgc gccgtccggg gcgaccagga tcgcgctgtt
    17821 gaaccggttt cctttctcgg tcagctcggc atagccaagg tagaagccga tgccgagctt
    17881 cttcgcctcg tcgaagagcg gctgggtctc tttcgacggc atggtctttt cgaagtaccg
    17941 ggcgtcgacc tcggcctggt cctccatcca ccagcgcggg aagaaggtgc tcaaggcgag
    18001 ctcggggaag accacgaact tcgcgcccat cgcgtgcgat tctctaagca aagccagcag
    18061 gcgcttgacc gcggcggcgc gcgtgtccga gcggttgagg ggtccgagtt gcgcggcggc
    18121 gattttcagg tgtcggctca tagcttcagg ggcgtgttcc atgccttgag ttgcgaaatt
    18181 tcggggacct cgcggcccag gggcttcagc gcctccgagg gctcgcgcgc gaggaagcgg
    18241 ccgcggccgc gctctgcgct gagtttgccg ttctcgatga tgatctcgcc tcgcgagagc
    18301 acggtgaccg gccagccggt cagcttgcgg cccttgtagg gcgtgtagcc ggtgttgtcc
    18361 cgggtctttt caatgaccag ttccctgtcc gggtcccaca ccgcgatatc ggcgtcggaa
    18421 ccgacggcga tggtcccttt ccgcgggtac aggccgtagg tctgcgcgtg gcgggcggag
    18481 gtgaggttca cgaactcttc cagggagatc cggcccgcct tgaccccgaa ggtgaaaagc
    18541 agcggcaggc gcagctcgag ccccggcacg ccgttcgcca tctccttgaa ggtggtcttg
    18601 tcgcccttcg gcagcttgcc ggaagcgtcc atgcggtagg gcgcgtggtc ggaggagtac
    18661 acgtgcagcg cgccgtcctt caggccgcgc cagcacgcct cctgcgcctt ttcgtcgcgc
    18721 ggcggcgggc tgcagcagaa catcgcgccc tgcaggccga tgtcgatgtc cttcgcggtc
    18781 aggaacaggt actgcgggca ggtctcgccg tagactttca gcccgttctt tcgcgcgctg
    18841 cggatcgctt cgatcccttc gggcgtggag acatgcacga tgagaatcgg acagtcgacg
    18901 agctcggcga gcttgatgac gcgctcgatc gcctcggcct ccgatgcgcg cgtgtggcag
    18961 atcgcgtggt agcgcggcag ggtgttcccc tgcttcacca tccgtcccgc gagccacgag
    19021 atcatgccgt ggttctccgc gtgcaccatc accagcgcgc cctcgcgccg ggccgccgcc
    19081 atcacgtcga ggatctgctc gtcgtgcagg cgcatgcggt cgtaggtcat gaagatcttg
    19141 aacgagcgga ttcccgagcg gatcgcctgc ggcaaatcca cagacaacgt tggctcgtcc
    19201 gggttggcga tgatcaggtg gaatccgtag tcgatgaccg cttttgcgcg tgccctctcg
    19261 tggtaatcct tcaatactgt tttcaagtca tcattgcgat gctgggcaca gaacggaatc
    19321 acggtcgtgg tgccgccgta tgcggcggaa actgtggcgg agtagaaatc gtccgccgcc
    19381 atgatcccca tgccggaaag ctgctcgatg tggcaatggc tgtcgacgcc gcccggaagg
    19441 atcagcatgt tctccgccgc gagcacgcgc ctgccagcga gattgcttcc gagtgcagcg
    19501 attttctcgc cggagatgcc gacgtccgcg cgaaacgtct tttccgcggt ggcgacggtg
    19561 ccgccgcgga tcaccaggtc gtgggtggtc attgcctttt gattatctca gccggccagg
    19621 aagccgccgt cgaccggcag cgcggcgccg gtcacgaagg aggattcatc gctcgcgagg
    19681 aacagcgccg ccgcggcgat ctcctcgggc cgcccgaacc gcccggcggg gtggcgctgg
    19741 cgcagcgttt cgcgcgccgc cttcgggtcc gcgcgccggg caaacgcgcg ctcgaggagc
    19801 ggcgtttcgg tggcgccggg cagcagcgcg ttggcacgga tgccgcggtc ggcgtagtcg
    19861 agcgccaccg atttcaccag gctgaccact gcgcccttgg acgcggcgta cgagcagttg
    19921 ctcctcgggc ctgcgaccgc gagctgcgac gcgatggcga tcagcgagcc gccgccgcgc
    19981 tcgagcattc cgggcagcac cgccttgaac cagaggaacg tgcctttcgc gttgaccgcg
    20041 aacacccggt cccagtcttc ctccgaggtg tcggcgagcg cttttccgac cgagatgccc
    20101 gccgcgcaga ccaggacgtc ggcggggggc agcttcggcg ccggccgcga cacatcggcg
    20161 accagggtct ggagcccagg gcgcggcttg atcggattca agtcgaccgc caggaccttt
    20221 gcgccctcgg ccagaaatcg ctcgacgatc gcggcgccga tcccggaggc tgccccggtg
    20281 acgacggccg tcttacccgc caaagacatg gatcgacttg cggcgtgcct cgtcgcgcgc
    20341 ttcttcgtag cccgcgtcgg cgtagcgcat cacgccggtg gcggtgtcgt tggtgagcga
    20401 gagctcgagg cgccggtcgg cctcgggcgt gccgtcggcg atcagggtga cgccggcgct
    20461 cgtcatgtag ccggcgtagc cgccgccgcc ggagtggatc gcgaccaggt cggccatcga
    20521 cgcgcagttg accatcgcgt tcagcagcgg ccagtcggcg atcgcatcgg agccgtcgag
    20581 catgcgctcg gtcatgatgt tcggatgcgc catcgcgccg gcgtcgaggt ggtcgcgcgt
    20641 gaacgcgatc ggcgcggaca gcaccttctt ccgcacgaga tcgttcaccg cgagcccgag
    20701 ccgcgtgcgc tcgccgtggc cgagccaggc gatgcgcgcc ggcaggccct ggaacggcac
    20761 ctgtgcgcgc gccaggcgga tccagttggc ggcgacgcgg ttttcgggaa atagctccag
    20821 caccaggtcg tcgatgcgcg cgatgtcctc gggcgtgccg ccgagcgcga tccagcggaa
    20881 cgggccgatg ccgcggctga agagcggccg caggaaagcc tcggagaaaa tcgggatgtc
    20941 gaaggcgtcg gcgaccccgc ccttctgcgc ctgcgtgcgg atcaggttgc cgttgtcgaa
    21001 caccaccgcg cc
//
LOCUS       DATBSZ010000140        21154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_362478, whole genome shotgun sequence.
ACCESSION   DATBSZ010000140 DATBSZ010000000
VERSION     DATBSZ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21154
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362478"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VIG70_09225"
     CDS             complement(<1..930)
                     /locus_tag="VIG70_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8250775.1"
                     /translation="MTFYRSIATAVLFFFIGAAAAQDLRIGLASEPTAMDPHYHNLSP
                     NNSLLSHIFESLVGQDERQRLTPDLAESWKAIDATTWEFKLRKNVRFHDGTPFTAEDV
                     VATYKRVPNVPNSPSSMATFTKPIVETKIVDPHTLIFKTAAPHVLLPSDLASVYIVPK
                     PIAEKATTDEFNSGKAAIGTGPYKFAEYIPNQRVVLKANYGYWGGEEPWDKVTFKILS
                     NSAARVAALLSGDVQMIETVPTSDIAKLATDKNFALADKVSNRVIYVHLHQWNDKTAP
                     FVTAKDGKPLDKNPFKDSRVRKALSMAINRDAIA"
     gene            complement(927..1592)
                     /locus_tag="VIG70_09230"
     CDS             complement(927..1592)
                     /locus_tag="VIG70_09230"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8250776.1"
                     /translation="METLEKYYARRAAEYEKIFDKPERQADLARMRKDIPALFKGERV
                     LEIACGTGYWTPLIAEQAGSVLATDFNEEPLDIARSKTYRKANVRFERADAYALPAWP
                     EKFSACYAGFWWSHIPLERIDEFLRNLAARLEAGAKVAFMDNRYVEGSSTPLSRKDAQ
                     GNTYQSRRLTDGSTHEVLKNFPTQQDMQQRLDRFGSDFGFSEYEYYWVATYRIMSPGR
                     TQK"
     gene            1610..2404
                     /locus_tag="VIG70_09235"
     CDS             1610..2404
                     /locus_tag="VIG70_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY8250777.1"
                     /translation="MAPLILDSPHSGHDFPADFGAIVSEAELRESEDCYVDELYAGAH
                     ELGVPLLAATFPRTYLDPNRNAGDVDLELIEGPWPWEYRPSGKARIGKSLIWRTLEDG
                     RPIYGRKLAPEAVRRRIERFHAPYHRSLQELLERATQKSGRVYHINCHSMRCVAGKQS
                     EDGPGSVRPDFVLGDRDGTTCEPRFTELVRETLAGMGYRVTVNDPYKGVELIRAYSDP
                     KAGRNSLQIEINKRLYMDEKTLAKTAGFARLQKDLGELLKVLVKSL"
     gene            2401..3348
                     /locus_tag="VIG70_09240"
     CDS             2401..3348
                     /locus_tag="VIG70_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEY8250778.1"
                     /translation="MKYEIEISPPDISAYRDGNSGVEYVHVLDSGKPGPAVMVQALTH
                     GNEFSGAIAVDFLFREKVKPLKGKLILAFANVAAFARFDFDDPDRSRYIDEDYNRVWA
                     DAVLGGSRDSAELRRARELRPFVDAADYLLDLHTMTEACRPIMVCGMLDKGAEFARRV
                     GVPADLLIDTGHPAGLRMRDRGGFGDPASAKNALLIEAGQHWEKSSVDVAIDTTLRFL
                     QATGITDAAWVHPRLRLQPPKEQRVVRVTQPVTAASTSFRFARPWKGLEVIEKAGTVV
                     ATDGEKVWRTPYDDCVLVMPSTAHVKPGNTMVRLGRYEG"
     gene            3350..3853
                     /locus_tag="VIG70_09245"
     CDS             3350..3853
                     /locus_tag="VIG70_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY8250779.1"
                     /translation="MRFFWLILLLAAMPASAQIYRWVDEKGTVHYSNARPPQGVKATV
                     FDTDAKAGPPSPESAECYTVRCQGERLEQRLARREELETRLAAERAAATPPRPRGLDF
                     RQYISLHRGMSEGELLGIAGAPDLLFSDVFASKNYTYLPTSGDPFTTTITVIGGRINH
                     IERIRKF"
     gene            complement(3850..3990)
                     /locus_tag="VIG70_09250"
     CDS             complement(3850..3990)
                     /locus_tag="VIG70_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250780.1"
                     /translation="MTAHHADFIAKLRQIEELAAGVHGELPQSLLVETAPSAIKPEKP
                     PA"
     gene            4143..4379
                     /locus_tag="VIG70_09255"
     CDS             4143..4379
                     /locus_tag="VIG70_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250781.1"
                     /translation="MEEQDRKGNSHDPVEGEGSYTGSKDYNQRTKKFVESGKVDQAAR
                     DAEPESEEEKQAMQKAERTGKERAKGEDPALRKK"
     gene            complement(4383..4970)
                     /gene="msrA"
                     /locus_tag="VIG70_09260"
     CDS             complement(4383..4970)
                     /gene="msrA"
                     /locus_tag="VIG70_09260"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY8250782.1"
                     /translation="MKKLIFVALLALALPAQAQKTEKASFAGGCFWCTEEAFEKVPGV
                     VSAVSGYMGGSVKKPTYEQVSTGRTGHTEVVEVTYDPAKVSYEKLLDTFWLNHDPTVK
                     DRQFCDSGSQYRPEIFWYTEEQKRLAEASKAKWEKQKPFRQPIVTPITKASEFWPAED
                     YHQDYYKKNALQYRFYVSGCGRYARLDSLWGSLRK"
     gene            complement(4967..5446)
                     /locus_tag="VIG70_09265"
     CDS             complement(4967..5446)
                     /locus_tag="VIG70_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEY8250783.1"
                     /translation="MRGNLLEEPVEAIVNAANGHLAHGGGVAGIIARAAGPALQKESD
                     ALVQKHGPFPTGSAVVTTAGKLPFKGVIHAVGPRQGEGDEEKKLVRALTAAFACAKDK
                     GWTSVSFPAVSSGIFAVPLDVCARAYLKAARACPLSKVRLCLRDQPVIDAVLKELGK"
     gene            complement(5470..5670)
                     /locus_tag="VIG70_09270"
     CDS             complement(5470..5670)
                     /locus_tag="VIG70_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEY8250784.1"
                     /translation="MARRYGKKAARKVRQAMHERKRGTLRSGRSGKKVTSRKQAIAIG
                     LSEARRAGAKVPRRKKSSRKKK"
     gene            complement(5679..6524)
                     /locus_tag="VIG70_09275"
     CDS             complement(5679..6524)
                     /locus_tag="VIG70_09275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8250785.1"
                     /translation="MNFRLLALAALAAPALVLAQAPLPGKDEPAKAPAKPAAKSAAKP
                     AAIATVNGVPVPQARADYLMQQQLQRGGADTEQMRSMVRDELVNREVLMQEAQRAGVA
                     KQADVQTQLDMARQEIIVSAYLREYARKNPVTDADVQKEYDKAKAQHGDKEYRARHIL
                     VETEDQAKTLIAELKKGGKFDDLASKNSKDTGSAQRGGDLDWNVPATYDKQFSDAMVK
                     LEKGKYTDTPVRTRFGFHIIQLDDVRPARFAALAEVKPRIQQMLVQTKIEELVKGLRA
                     KAKVE"
     gene            complement(6521..6799)
                     /locus_tag="VIG70_09280"
     CDS             complement(6521..6799)
                     /locus_tag="VIG70_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY8250786.1"
                     /translation="MSVAARIRERLAALEPVNLELVDESAQHAGHAGAQPGGNTHWRL
                     TIVSPRFAGQPTVARHRMVYQALGELMQNPIHALAITARAPEETKGSQ"
     gene            complement(6774..7325)
                     /locus_tag="VIG70_09285"
     CDS             complement(6774..7325)
                     /locus_tag="VIG70_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEY8250787.1"
                     /translation="MKFLFDFFPVILFFIAFKFADIYVATGVAIAATFVQVGWLKLRG
                     KRVEPMLWASLAIIAVFGGATLVLQDETFIKWKPTVLYWLFGAVLAGAAAFRRNLIRA
                     MLSEQMQLPEAVWSRLNWSWIGFFAFMGALNLYVAYNYSTDHWVNFKLFGGMGLMLIF
                     VFAQAMVLSRFIEEKDERRSENP"
     gene            complement(7339..8691)
                     /locus_tag="VIG70_09290"
     CDS             complement(7339..8691)
                     /locus_tag="VIG70_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEY8250788.1"
                     /translation="MESWPHRDLWTGVVFNGEKIGFTRRQVRAAADAPGRWEIESEAV
                     MRLQFLGVDKRIRLHAVDRVRPDLTLEVFSYEHEIDGNRMQVRGTAEGAALSFAVEAN
                     GSAENKTLKVEKPLYPTSALAFLPLIQGIKIGTRARHRVFHGETQAIADAEQDVLAYE
                     TSPLFEGPAFKVATRLLGLETTTWIAADGRPLLELGMRGAIVSALEDEARARHYLVEA
                     SLNKRDAMVEFSLLRAGPIEQPRRVSRMDIVLDDFPKAFAVDNGACRRVSDRLECRID
                     RSAPLAQGDPQRYLAPTLAAPSVLGEIRDLASEINKGSTSEEQTIARLLAWMDANIAK
                     ETVDAFTAADVLRARRAECQGHAYLFAAFARALGIPARIVNGIAYSEPHGGFLYHTWN
                     EAWIAGRGWQPVDATFGQALADATHVKLIEGESAAELVPLVNLVGRVRVASVSALARW
                     "
     gene            complement(8787..9470)
                     /locus_tag="VIG70_09295"
     CDS             complement(8787..9470)
                     /locus_tag="VIG70_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250789.1"
                     /translation="MIDWLAGLDPRALMIGIGLVGLLLAVPLELAAIRRLRHLQLARG
                     TLFFLSGAFVLALAAAMALVAMNLYTYARLTHEQEAARISMRQLGERHYVVSVQAKKT
                     PPRHYELRGDEWQIDARVLKWRAMGNLLGFDTVYRLERLSGRYGDITLERKAPRTVHS
                     LAEDPGLDFWSLLRKHHEYVPLADALYGSAAYVPMAEGAEYAVTVSSSGLVVRPGNDA
                     ARKAVGGWK"
     gene            complement(9467..9784)
                     /locus_tag="VIG70_09300"
     CDS             complement(9467..9784)
                     /locus_tag="VIG70_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250790.1"
                     /translation="MNGLRLVLALAMGATSLSAAARCEFPDEGNMPLRRAVSRVEMLP
                     EVHEWHRSMVEKGSKPQYVVRLDEPVREAGKCYWPVEVRADGKLWRRYFISPDGKKFL
                     VKG"
     gene            9964..13614
                     /locus_tag="VIG70_09305"
     CDS             9964..13614
                     /locus_tag="VIG70_09305"
                     /inference="COORDINATES: protein motif:HMM:NF040192.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HEY8250791.1"
                     /translation="MREKSVSGAPGLGAVFLGAFANLFRSRARARRALGNPNRGRASP
                     LRFEGLEQRLLLSADLACSPVDPTFDNGDDLVLYDADGTQVTFKLHGAGNGAVVADED
                     GGWDVVLVNTNANTTFSVKARGGDGQVDLDDIRVGGAIGEIDAGDVHLTGTLAVDGAL
                     GAGLVLGSASDALVAAPSVGINFFGLNLGGIIILGDLEDSEILIGAHLGADGQPGGTG
                     PAADAYGRGELTLLTILGSVEGSAVRVGQDPVNGVLDDCDDVIRGGAGSSIGLILIGG
                     ELSADSRIIAGDLPAWAWVDGRLIRTADDPRFKTDVHGPDLELALANDTGASTADGLT
                     RDPSIAGDASDAGGIAALRAGFGGTATVDITDELAADGFFLLSRSDLEEIYGAPLVDG
                     SYTLTIEATDAQGNACPQNISFTLDSTSPTVTIDQAAGQEDPASDGPILFTVEFSEAV
                     TGFSGADLSFAGSTAGSELVAEVTGSGSTYTVSVTGMSGAGTVVVSVPAAAAADAAGN
                     TSGASTSTDNSVTVRTNQAPVAGDDFFSSRPGRTEVITFEQLLDDDTDADGDALRVVD
                     VDRQMLDEDGNVLAEVELEEGQLRVTPSGLFIGAGRFSYRIADDSGGFDAGEVTITYF
                     NQAPVAGEDLFFTRPGQTETITFQQLLDDDTDADGDALRVVDVGRVVLDAMGNVVAEV
                     IEVNFEQGTLVVAPAELFTGAGHFSYRITDDFGGVDDGDVTITYVNQEPVAGDDAFSS
                     RPGQTEVITFEQLLDDDMDADGDALRVADVDRQVLDENGNVVAEVELEEGQLRVTPTG
                     LFVGAGRFSYRIGDGHGGFDQGEATITYANQAPVAGEDLFFWRPGQTEVITFEQLLDD
                     DTDADGDALRLVDVDRVVRDAMGNVVAEVVEVNFEQGTLVVAPAEFFTGAAHLSYRIT
                     DDFGGIDDGDVTITYIDNDPPVTDEAFIELPAGEVGVITFAQLLEGDFDPNGDVLRVE
                     SAGSRVMDVMGNVVATVEMDFEQGRLVVTPTTFFTGAGRFNYRIADGNGGFDDGEVTL
                     IFTGTAPEETQAGVTLLGDSPELQSIFDASLAAWSSMLGLDAAVVQELDAMPVEVVDL
                     VDGSLAYTDGTTIFIDADAAGHGWFVDATPLASEEYELVDVGTWQALEFGRADDAVDL
                     FTVVTRQLGHLLGFDHESGWTVMQPGTVAPGMRYTIDWNAAAGASFSGLPPSTLNRSF
                     GASVADFVRAVT"
     gene            13674..14558
                     /locus_tag="VIG70_09310"
     CDS             13674..14558
                     /locus_tag="VIG70_09310"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8250792.1"
                     /translation="MDARLSPGVGILLLLACSTLFASNHVAARIAFDHGASVAAAVAL
                     RAAGTALVLFCMLRVQGIPLTLPRGLRGPALVAGVLVAVQSYCLFSAVALIPAALALL
                     VFQTSVLLYVLICWASGRETPRPRSLAAMALALIGLALVLDVRPAHFAASWAQLGAGV
                     LWAFGGAVSFAFVYYANSYVLKPLDGRLRALVMTSVTTVLAVAGGAAADALAVPRDGI
                     GWVGLALVTVFYCIALSLLFVVLPKLPPTSSVALNFEPIALLALAWVFLGQAVTSAQI
                     AGAFVTVGAIAWMGLMKK"
     gene            complement(14560..15690)
                     /locus_tag="VIG70_09315"
     CDS             complement(14560..15690)
                     /locus_tag="VIG70_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8250793.1"
                     /translation="MNKRASRRRFLAGAAAGSATLGFPAIVQAQAPVTLRFQSTWPVK
                     FIYHEAAVDWTKKVAEITNGRLKVDMLPAGAVVPGLQVIDAVNKGVLDGGHGIPAFWF
                     GKNTAFGLFGAGPDFGMDAMQMLGWIEYGGGRELYAEIQAAANLDIFSFLLGPVPCEP
                     FGWFKKEIRGLKDLKGLKIRTSGLAIGNYNALGMTAVQLAPADIVPSLDRGVIDAAEF
                     ATAYDDRIMGFPDVAKFYLQQSYHMANNVCEIMINKKKYEALPEELKALLKHASHAAS
                     ADMWWKGMHRHSQSYLDLQTKDKVRAYRTPREILNAQLKAWDGVIAKGAAENPLFAKV
                     IESQKAWARRVMFWHNDIQVDQRAAYAHYFGKGPLAAKPAGA"
     gene            complement(15687..16103)
                     /locus_tag="VIG70_09320"
     CDS             complement(15687..16103)
                     /locus_tag="VIG70_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8250794.1"
                     /translation="MSVKTAAVKRKPKIEPTIGASTYVKPDQIDWKPSPFPGIQIKVL
                     YEDKAKGEMTCLLKWEPGASLPMHRHPELEQTWVLEGSFYDHDGICKAGEYVWRKPGS
                     FHETHSDTGAVILAIYRKPNVFYRKTGEEVSSGYDT"
     gene            16176..16799
                     /locus_tag="VIG70_09325"
     CDS             16176..16799
                     /locus_tag="VIG70_09325"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY8250795.1"
                     /translation="MRFAQFIYDGVEPVEMAIYGVLSMARRIAPQIEMFTVAPRAGVV
                     QLANGLRVLADHAMSACPEADVLIVCGGPGWSREAANPATLEFLRTFRPRQALASVCT
                     GGMILAATGLLDGRHATTKRDGFEGEPSPLALMRQKHPAIKALEVRLVDEGNLITGGG
                     VTLCIDATLHLLRRFVGEDVAVKTARAMAYSNAWEANRLAFGEWSGT"
     gene            16784..17368
                     /locus_tag="VIG70_09330"
     CDS             16784..17368
                     /locus_tag="VIG70_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY8250796.1"
                     /translation="MVRHLKVRSSIGERIARLRADRRMTLDALARATGFTKSYLSKIE
                     NARKVPPIGSLSRIAVALDSDIAGFFDSGPGMRDAGACVVRAGERRPVVRGGTAFGYD
                     YLSLAYRKHDKKMEPFIFTFPAKIDSHVFFDHAGEEFLFVLKGRIEFQVGGERWVLRE
                     GDSLYFDSHIPHRGWSLGGAAKVLVVIYKSEPRK"
     gene            complement(17369..19096)
                     /locus_tag="VIG70_09335"
     CDS             complement(17369..19096)
                     /locus_tag="VIG70_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HEY8250797.1"
                     /translation="MQSKLRFAPAAVEVENRRDGSIVLRSPQKLGFYARCVTEWLVKW
                     SDQAPDRTFLAERSGDRWKKLTYRETYGAVRRIGQALLDRNLNAERPVAILSDNGIDH
                     ALLALGAMHVGVPVAPVSPAYSLMSKDFGKLKTIFELVKPGLVFAADPQKFAPALAAV
                     GAKSVSVAELLETNPGSTQEREFAKVKPDTVAKILFTSGSTGIPKGVVNTHRMLCANQ
                     QMLAQAWPFVEERAPVVVDWLPWNHTFGGNHNFNLVLRNGGTMYVDGGKPAPGLVETT
                     VRNLKEVAPTMYFNVPRGYDLLLPFLEKDAELRRNFFRDLDVLFYAAAALPQNLWDRL
                     IRLSKDEENTKLAMLSAWGSTETSPLATSVHFPMERAGVIGLPVAGCELKLVPAAGKL
                     EVRVRGQNVTPGYHKRADLTQAAFDEEGFYRIGDAVRFADPSDPAKGIVFDGRVAEDF
                     KLSTGTWVHVGALRIALISAGDPLIQDAVICGHDRSEVGALVFLSPAAKDLAPQEIRA
                     RLSNALKKMQGTSSTRPARLLVMTEPPSIDANEITDKGYMNQRAVLERRAALVEKLYA
                     GGEGLITAT"
     gene            complement(19268..19921)
                     /locus_tag="VIG70_09340"
     CDS             complement(19268..19921)
                     /locus_tag="VIG70_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250798.1"
                     /translation="MLRAACLSAALLACAPALAQNYAGTYTAKNPSGGTLMLTLAHED
                     QKRVSGTLTGHGRSSLQVQARVEADGLRGTAANNFGMLHLTGRLVGEELKMVLTEPDL
                     GGKPNPQRATELRLAKTETKSTPQSQRLSQALTASPWCAAAYDISGKRGLERLVFMPN
                     GLVNQMPGGQARWRVQNETLEFSRDGFSWSPQPLRLALSSNGSPVLQSQGKEYLQCD"
     gene            complement(19951..20187)
                     /locus_tag="VIG70_09345"
     CDS             complement(19951..20187)
                     /locus_tag="VIG70_09345"
                     /inference="COORDINATES: protein motif:HMM:NF040052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6306 domain-containing protein"
                     /protein_id="HEY8250799.1"
                     /translation="MIRLDPGYDYRRSLSPETGVGYSHATGEFYSKAVAIKGSRARIG
                     FLVRAPKWEVKRFEKLPEDLAGVTHVASAARDSA"
     gene            20198..21076
                     /locus_tag="VIG70_09350"
     CDS             20198..21076
                     /locus_tag="VIG70_09350"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY8250800.1"
                     /translation="MDKQLQVVDQVTSAGIDMAIRFGPKVLVAALILVAGFLAGRWVS
                     RALGRALAKFQLEPPVLALIARVTHLLVLGLFVIMALQNLGVELLPLIAGVGIAGAGI
                     ALAMQGVLGNVVAGLTIIFTRPFRVGDYISIVKEEGEVLEIRLFSTTLGHADLSKVVI
                     PNRKIVGEILHNYGRVRQLPIEVGVSYETDIGAALKLAEEILRANPRVLKDPAPVTKV
                     LKLADSSVVIAAAPWVAVPDYGAASGELHQAILEGFRRRNIVIPLPQREIRIASSSAY
                     VAAPGAVGRLAGRDAA"
BASE COUNT         3384 a         6858 c         7178 g         3734 t
ORIGIN      
        1 ggcgatcgcg tcgcggttga tcgccatcga cagggccttc ctcacgcgag agtccttgaa
       61 cggattcttg tccaggggct tgccgtcctt cgcggtaacg aacggcgccg tcttgtcgtt
      121 ccactggtgc aggtgcacgt agatcacgcg gttggaaacc ttgtccgcga gcgcgaaatt
      181 cttgtcggtc gcgagcttcg cgatatcgga ggtgggtacc gtctcgatca tctgcacgtc
      241 gcccgacagc agcgcggcca cgcgcgccgc ggaattggac aggattttga acgtgacctt
      301 gtcccacggc tcctcgccgc cccagtagcc gtagttggcc ttcagcacca cgcgctggtt
      361 ggggatgtac tcggcgaact tgtagggccc ggtgccgatc gccgccttgc ctgaattgaa
      421 ttcgtcggtg gtggccttct cggcgatggg cttgggcacg atgtagaccg aagcgaggtc
      481 gctcggcagc agcacgtgcg gcgccgcggt cttgaagatg agggtgtgcg ggtcgacgat
      541 cttcgtttcc acgatgggct tggtgaaggt cgccatcgac gaggggctgt tgggaacgtt
      601 gggcacgcgc ttgtaggtcg cgaccacgtc ctcggcggtg aacggcgtgc cgtcgtggaa
      661 gcgcacgttc ttgcgcagct tgaactccca ggtggtggcg tcgatcgcct tccacgactc
      721 ggcgaggtcg ggggtcaggc gctggcgctc gtcctggccg accaggctct cgaagatatg
      781 cgagagcagt gagttgttcg gcgacaggtt gtggtagtgc gggtccatcg ccgtcggctc
      841 ggaggcgagg ccgatgcgca ggtcctgcgc ggcggccgct ccaatgaaaa agaacagcac
      901 tgccgtggcg atcgagcgat agaaagtcat ttttgcgtcc tccctggaga cattatgcga
      961 taggtcgcga cccagtagta ctcgtactcg gagaagccga agtcgctgcc gaagcggtcc
     1021 aggcgctgct gcatgtcctg ttgtgtcggg aagttcttca gcacttcgtg ggtcgagccg
     1081 tcggtgagcc ggcggctctg gtaggtattg ccctgcgcgt ccttcctcga gagcggcgtg
     1141 ctcgagcctt cgacgtagcg gttgtccatg aacgccactt tcgcccccgc ttccagcctt
     1201 gcagcgagat tgcgcaggaa ttcgtcgatg cgctccaaag ggatatgaga ccaccagaac
     1261 ccggcgtagc acgccgagaa cttctccggc caggcgggca gggcgtaggc gtcggcgcgc
     1321 tcgaagcgca cgttcgcctt gcggtaggtc ttcgatcggg cgatgtcgag cggctcctcg
     1381 ttgaagtccg tcgccagcac cgacccggcc tgctcggcga tgagcggcgt ccaatagccg
     1441 gtgccgcacg cgatctcgag cacgcgctcg cccttgaaga gcgcggggat gtccttgcgc
     1501 atgcgtgcga ggtcggcctg gcgctcgggc ttgtcgaaga tcttttcgta ttccgccgcc
     1561 cggcgggcgt agtacttttc cagggtttcc attgaagtat cctaccgtga tggccccact
     1621 gatactcgat tccccgcact cggggcacga ctttcccgcc gatttcggcg ccatcgtgag
     1681 tgaagccgag ctacgtgaat cggaagactg ctacgtcgac gagctctatg ccggtgcgca
     1741 cgagctcggc gtgccgctcc ttgccgccac ctttccgcgg acctacctcg acccaaatcg
     1801 caacgccggc gacgtcgacc tcgagctgat cgaagggccc tggccgtggg agtaccggcc
     1861 gagcggcaag gcgcgcatcg gcaaatccct catctggcgc acgctggaag acggccgccc
     1921 gatctacggg cgcaagcttg cgcccgaggc cgtgcggcga cggatcgagc gctttcatgc
     1981 gccctatcat cgttccctgc aggagctgct ggaacgcgcg acccagaaat cgggccgggt
     2041 gtaccacatc aattgccact ccatgcgctg cgtggccgga aagcagagcg aggacggccc
     2101 cggcagcgtg cgccccgact tcgtcctcgg cgaccgcgac ggcacgactt gcgagccgcg
     2161 cttcaccgag ctggtacgcg agacgctcgc cggcatgggt taccgcgtca cggtgaacga
     2221 cccgtacaag ggcgtcgagc tgatccgcgc ctactccgac cccaaggcgg ggcgcaacag
     2281 cctgcagatc gagatcaaca agcggcttta catggatgag aagacactgg cgaagaccgc
     2341 cggcttcgcc cggctgcaga aggacctcgg agagcttctc aaggtgctgg tcaaatccct
     2401 gtgaaatacg agatcgagat ctcgccgccg gatatttccg cctaccgcga cggtaatagc
     2461 ggcgtggaat acgtgcatgt cctcgactcg ggaaagcccg ggccggcggt gatggtccag
     2521 gcgctcaccc acggcaacga gttttccggc gcgatcgctg tcgattttct ttttagggag
     2581 aaagtaaaac ctctgaaggg aaagctgatc ctcgcgttcg ccaacgtggc ggcgttcgca
     2641 cgcttcgact tcgacgatcc cgaccgctcg cgctacatcg acgaggacta caaccgcgtc
     2701 tgggccgacg cagtgctcgg cggctcgagg gattctgcgg agctcaggcg cgcgcgcgag
     2761 ctgcgcccgt tcgtcgatgc cgccgattac ctgctcgatc tgcacaccat gaccgaagcg
     2821 tgccggccga tcatggtgtg cggcatgctc gacaagggcg ccgagttcgc gcggcgcgtc
     2881 ggcgttccgg cggacctgct gatcgatacc gggcatccgg ccggcctgcg catgcgcgac
     2941 cgcggcggct tcggcgatcc ggcgagcgcg aagaacgcgc tcctcatcga agccggccag
     3001 cactgggaga aaagctcggt cgacgtggcg atcgacacga cgttgcgctt cctgcaggcc
     3061 actgggataa cggatgcggc atgggtgcac ccgaggctaa ggctccagcc cccgaaggag
     3121 cagcgcgtcg tgcgggtgac ccagccggtc accgccgcga gcacgagctt ccgcttcgcc
     3181 aggccctgga aaggcctgga agtcatcgaa aaagccggca ccgtggtggc gaccgacggc
     3241 gaaaaggtct ggcgcacccc ctacgacgat tgcgtgctcg taatgccctc cactgcgcac
     3301 gtcaagccgg ggaatacaat ggtgcgattg gggcgttatg agggttgaga tgcggttctt
     3361 ttggctgatc ctgctcctcg cggcgatgcc cgcgtccgcc cagatctacc gctgggtgga
     3421 cgagaagggc acggtccact actccaacgc gaggccgcca cagggcgtga aggccacggt
     3481 gttcgatacc gacgccaagg ccgggccgcc ctcgcccgaa agcgcagagt gctacaccgt
     3541 gcgctgccag ggcgagcgtc tcgagcagcg cctcgcgcgg cgcgaggagc tggagacccg
     3601 gctcgcggcg gagcgcgctg cggccacgcc gccgcgcccg cgcggcctcg acttccgcca
     3661 gtacatctcg ctccaccgcg gcatgagcga gggcgagctg ctcggcatcg ccggcgcgcc
     3721 cgacctgctg ttctcggacg tgttcgcgtc caagaactac acctatctcc ccaccagcgg
     3781 cgatccgttc accaccacca tcacggtgat cggcggccgc atcaaccaca tcgagcgcat
     3841 ccgcaagttc taggcgggcg gtttttcggg cttgatcgca gacggcgcgg tctcgactaa
     3901 caggctctgc ggcagctcgc cgtggacgcc ggcggcgagc tcttcgatct ggcgcagctt
     3961 ggcgatgaaa tcggcgtggt gcgcggtcat gccgctcccg gaaaattcag aacgccgaag
     4021 cgtacgcgcg cggacgcacg gtgaatgtcg cgcgtttggt gcttccgctg tcacggatcg
     4081 gacttcatgc ttcgggcacg gcgcttgctg ctggaggtgc ggataccttc aggaggtccg
     4141 tcatggaaga gcaggatcgc aaaggcaact cccacgaccc ggtcgaggga gaaggcagct
     4201 acaccggcag caaggactac aaccagcgca cgaagaaatt cgtcgagtcg ggcaaggtcg
     4261 accaggccgc gcgcgacgcc gagccggagt ccgaggaaga gaagcaagcg atgcagaagg
     4321 ccgagcgcac cggcaaggag cgcgcgaaag gcgaagaccc ggccctgcgg aagaagtgat
     4381 ttctatttcc tgaggctgcc ccagagcgaa tcgagccgcg cgtagcggcc gcaaccgctc
     4441 acatagaacc ggtactgcaa ggcattcttc ttgtagtaat cctggtggta gtcctccgcc
     4501 ggccagaatt cgctcgcctt ggtgatcggc gtgacgatcg gctggcggaa gggcttctgc
     4561 ttttcccact tcgccttgga ggcctcggcg aggcgcttct gctcctcggt gtaccagaag
     4621 atctcgggcc gatactgcga gcccgaatcg cagaactggc ggtccttcac cgtcgggtcg
     4681 tggttcagcc agaaggtgtc gagcagcttc tcgtagctca ccttggcggg atcgtaggtg
     4741 acctcgacga cctcggtgtg gccggtacgg ccggtcgaca cctgctcgta ggtcggcttc
     4801 tttacgctgc cgcccatgta gcccgagacc gcggacacca cgccgggcac tttctcgaac
     4861 gcttcctcgg tgcaccagaa gcagcccccc gcgaagctcg ctttttcggt cttctgcgcc
     4921 tgtgcgggca gggcgagcgc cagcagcgcc acgaagatca gttttttcat ttccctagct
     4981 cctttaggac ggcatcgatg accggttggt cgcgcaagca tagccggacc ttactcagcg
     5041 ggcaggcgcg cgcagccttg aggtaggccc gcgcgcagac atccagcggc actgcgaaga
     5101 tcccgctgga gactgccggg aacgagaccg aggtccagcc cttgtctttc gcgcaggcaa
     5161 aggctgcggt aagcgcacgc acgagcttct tttcttcgtc gccttcgccc tggcgcggac
     5221 cgacggcgtg gatcacgccc ttgaaaggaa gctttccggc ggtggtgacg accgccgagc
     5281 cggtcggaaa ggggccgtgc ttctggacga gagcgtccga ttccttctgc agcgccggcc
     5341 ccgcggcgcg cgcgatgatg ccggccacgc cgccgccgtg ggcgaggtgg ccgttggccg
     5401 cgttgacgat cgcctccact ggctcttcca ggagattgcc gcgcaccacc tggaacaggc
     5461 ggccgggcgc tacttcttct tgcgtgagga cttcttgcgt cttggcacct ttgcgccggc
     5521 gcggcgcgcc tcggacagcc cgatcgcgat cgcctgcttg cggctggtga ccttctttcc
     5581 ggacctgccg ctgcgcagcg taccgcgctt gcgctcgtgc attgcctggc ggactttgcg
     5641 agctgccttc ttcccgtatc tgcgggccat gaaacctcct actccacctt tgccttcgcc
     5701 cttagcccct tcaccagctc ctcgatcttg gtctgcacca gcatctgctg gatgcgcggc
     5761 ttgacttcgg ccagcgccgc gaacctcgcg gggcgcacgt cgtcgagctg gatgatgtgg
     5821 aagccgaagc gcgtgcgcac cggcgtgtcc gtgtacttgc ccttttcgag tttgaccatg
     5881 gcgtcggaga actgcttgtc gtaggtcgcc ggcacgttcc agtcgaggtc gccgccgcgc
     5941 tgcgcgctgc cggtgtcctt ggagttcttg ctcgccaggt cgtcgaactt gccgcccttc
     6001 ttgagctccg cgatcagcgt cttcgcctgg tcctcggtct cgaccaggat gtggcgcgcc
     6061 cggtattcct tgtcgccgtg ctgcgccttg gccttgtcgt actccttctg aacatcggcg
     6121 tcggtgaccg ggttcttgcg cgcgtactcg cgcaggtacg cgctgacgat gatctcctgg
     6181 cgcgccatgt cgagctgggt ctgcacgtcg gcctgcttgg cgacgccggc gcgctgcgcc
     6241 tcctgcatca ggacctcgcg gttcactagc tcgtcgcgca ccatgctgcg catctgctcg
     6301 gtgtcggcgc cgccgcgctg caactgctgc tgcatcaggt agtcggcgcg cgcctgcggc
     6361 accggcacgc cgttgaccgt ggcgatggcg gccggcttgg cggcggattt cgccgcgggc
     6421 ttggcgggcg ccttggccgg ctcgtccttg ccgggcagcg gcgcctgcgc gaggaccagg
     6481 gcgggggccg ctagcgcggc aagtgcgaga aggcggaaat tcactggctt cctttcgttt
     6541 cttcgggtgc gcgcgcggtg atcgcgagcg cgtggatggg attctgcatc agctcgccga
     6601 gcgcctgata gaccatgcgg tggcgcgcga ccgtcggctg ccccgcgaag cgcggcgaca
     6661 cgatggtgag ccgccagtgt gtgttgccgc cgggctgcgc gccggcgtgg ccggcgtgct
     6721 gcgcgctctc gtcgacgagc tcgaggttca ccggctcgag cgccgcgagc cgctcacgga
     6781 ttctcgctgc gacgctcatc cttctcttcg atgaaccggc tcagcaccat cgcctgggcg
     6841 aacacgaaga tcagcatcag ccccatgccg ccgaacagct tgaagttgac ccagtggtcg
     6901 gtggagtagt tgtaggccac gtacaggttg agcgcgccca tgaaggcgaa gaacccgatc
     6961 cagctccagt tgagccgcga ccacaccgcc tcggggagct gcatctgctc ggacagcatg
     7021 gcccggatca ggttccggcg aaaggcagcc gctcccgcga gcaccgcgcc gaacagccag
     7081 tagagcacgg tgggcttcca cttgatgaag gtctcgtcct gcaggacgag agtggcgccg
     7141 ccgaacaccg cgatgatcgc gagactcgcc cacagcatcg gctccacgcg cttgccgcgc
     7201 agcttgagcc atccgacctg gacgaaggtc gcggcgatcg ccaccccggt cgcgacgtag
     7261 atgtcggcga acttgaaggc gatgaagaaa aggataacgg ggaaaaagtc gaacaggaat
     7321 ttcatgcccg gtcggagttt accagcgagc gagcgcggaa accgacgcca cgcgcacgcg
     7381 accgaccagg ttcacgagcg gcacgagctc ggccgcgctt tcgccctcaa tgagcttgac
     7441 gtgcgtggcg tcggcgagcg cctggccgaa ggtcgcgtcg accggctgcc agccgcgccc
     7501 cgcgatccag gcctcgttcc aggtgtggta gaggaagccg ccgtgcggct cggagtaggc
     7561 gatgccgttc acgatgcgtg ccgggatgcc gagcgcgcgc gcgaaagcgg cgaacaggta
     7621 ggcgtgaccc tggcattcgg cgcgccgggc gcgcagcacg tcggcggcgg taaaggcatc
     7681 gactgtttcc ttcgcgatat ttgcgtccat ccacgcgagc aggcgagcga tggtctgctc
     7741 ttcacttgta cttcctttgt tgatttcgct cgcaagatcc ctgatctccc cgagcacgct
     7801 cggcgcggcg agcgtcggcg cgaggtagcg ctgcgggtcg ccctgcgcaa gcggcgcgct
     7861 gcggtcgatg cggcattcca gccggtcgga cacccggcgg cacgcgccgt tgtcgactgc
     7921 aaaagccttg ggaaaatcgt cgaggacgat atccatgcgc gacacccgcc gcggctgctc
     7981 gatcggcccg gcgcgcaaca ggctgaactc gaccatcgcg tcgcgcttgt tgaggctcgc
     8041 ctcgaccagg taatgccgcg cgcgcgcttc gtcctccagc gccgaaacga ttgcaccgcg
     8101 catgccgagc tcgagcagcg gccggccgtc ggcggcgatc caggtggtgg tctccagccc
     8161 gaggaggcgg gtcgcgacct tgaacgccgg cccctcgaac agcgggctcg tttcgtaggc
     8221 gagcacgtcc tgctcggcat cggcgatcgc ctgggtctcg ccgtggaaca ccctgtggcg
     8281 cgcgcgggtc ccgatcttta tcccctgtat caacggcaga aaggccagcg cgctggtcgg
     8341 atacagcggc ttttcgactt tcagcgtctt gttctcggcg ctgccgttcg cctccacggc
     8401 aaagctcagg gcggcgcctt cagcggttcc gcgcacctgc atgcggttgc cgtcgatctc
     8461 gtgctcgtag ctgaagacct cgagcgtcaa gtccggacgc acccggtcga cggcgtgcag
     8521 gcggatgcgc ttgtcgacgc cgaggaactg caggcgcatc acggcctcgg actcgatctc
     8581 ccagcgcccg ggcgcatcgg cggcggcgcg cacctgccgc cgcgtgaagc cgatcttctc
     8641 gccgttgaac accaccccgg tccacaggtc gcggtgcggc cagctttcca aggtgacggc
     8701 gccgggctgc gggggcggcc ctgcgtcgcg aaagtactgg ctcgcacacc cctgaagaca
     8761 gaggacgagg acaagaaccg cgaccctcat ttccacccac ccaccgcttt gcgcgccgcg
     8821 tcgttgccgg ggcgcaccac aagcccgctg ctcgacacgg tgactgcgta ttcggcgccc
     8881 tcggccatcg gcacataggc ggcgctgccg tagagagcgt cggcgagcgg cacgtattca
     8941 tgatgttttc taagcaaaga ccaaaagtcg agaccggggt cctctgcgag ggagtgcacg
     9001 gtgcgcggcg ccttgcgctc cagcgtgatg tctccgtagc gccccgagag ccgctcgagc
     9061 cgatagaccg tgtcgaagcc gagcagattg cccatcgcgc gccacttcag cacgcgcgca
     9121 tcgatctgcc attcgtcgcc gcgcagctcg taatggcgcg gcggggtctt cttcgcctgg
     9181 acgctcacga cgtagtggcg ctcgccgagt tggcgcatcg agatgcgcgc tgcctcctgc
     9241 tcatgggtca ggcgtgcgta ggtgtacagg ttcatcgcga cgagggccat ggcagccgcc
     9301 agcgccagga caaaggctcc cgagagaaag aacagggtgc cgcgcgcaag ctgcaggtgg
     9361 cgcaggcgcc ggatggcagc gagctcgagc ggcacggcga gcagcagccc gacgagcccg
     9421 atgccgatca tcagcgcgcg cgggtcgagg cccgcaagcc agtcgatcat cccttgacca
     9481 ggaacttctt gccgtcgggc gagatgaagt agcgccgcca cagcttgccg tcggcgcgca
     9541 cctcgaccgg ccagtagcac ttgccggctt cgcgcaccgg ctcgtcgagc cgcaccacgt
     9601 actgcggctt cgagcctttc tcgaccatcg agcgatgcca ctcgtgaacc tcgggcagca
     9661 tctcgacgcg ggacacggcg cggcgcagcg gcatgttgcc ttcgtcggga aactcgcagc
     9721 gcgcagcggc ggacaggctg gttgcgccca tcgccagggc taacactagt cttaagccat
     9781 tcatgggatt cgatcatata tgtggccgtg atgtagttct tgctgcaccg cgcgcattcg
     9841 cgcactaagt cgcaacctgt ccccgggcga cgatcgcctc gtgacttatg cagcggcaac
     9901 cgccaaacgg cggtgcctag actgggaccg tctccgaatg cagccatctg taggggaggt
     9961 cgcatgagag agaaatcggt ttcgggcgcg cccgggctgg gcgcagtctt cctcggcgct
    10021 ttcgccaatc ttttccgcag tcgcgcccga gcgcgcaggg cgctcggcaa tcccaaccgg
    10081 ggtcgcgcgt cgccgttgcg gttcgaaggg ctggaacagc gcctgctgtt gtcggcggac
    10141 ctggcttgct cgcccgtcga cccgaccttc gacaacggcg atgacctggt gctgtacgac
    10201 gccgacggca cgcaagtcac tttcaagctg cacggtgccg ggaacggcgc agtcgtcgcc
    10261 gacgaggatg gcggttggga tgtggtcctc gtcaacacca acgcgaatac caccttttcg
    10321 gtgaaggcgc gcggcggcga cgggcaggtc gacctcgacg acatccgcgt cggcggcgcg
    10381 atcggcgaga tcgatgcggg cgacgtgcac ctcaccggca cgctggcggt cgacggcgcg
    10441 ctgggcgcgg ggcttgtgct cggcagcgcg agcgatgcgc tggtcgcagc gccgagcgtg
    10501 ggcatcaatt tctttggact gaacctgggc ggcatcatca tcctcggaga cctcgaggac
    10561 tcggaaatcc tgatcggcgc gcatctcggc gccgacggcc agcccggcgg cacgggcccc
    10621 gcggcggacg cgtacggacg cggcgagctc acccttctca ccatcctcgg ctcggtcgaa
    10681 ggcagcgccg tgcgtgtcgg gcaggacccg gtgaacggcg tgctcgacga ctgcgacgac
    10741 gttatccgcg gcggcgccgg cagcagcatc ggactgatct tgatcggcgg cgagctgagc
    10801 gccgacagcc gcattatcgc gggcgatctt cctgcgtggg catgggtcga cggccggctg
    10861 atccgcacgg cggatgaccc gcgcttcaag accgacgtcc acggcccgga cctggagctc
    10921 gcgctggcga acgataccgg ggcttcgacg gccgacggcc tgacccgcga tccgtcgatc
    10981 gcgggcgacg ccagcgacgc gggcggcatc gccgccctgc gggcgggctt cggcggcacg
    11041 gcgaccgtcg atatcacgga cgagcttgcc gccgacggct tcttcctcct ctcccgctcc
    11101 gacctcgagg agatctacgg cgcgccgctc gtcgacggct cgtacacgct caccatcgaa
    11161 gcgaccgatg ctcaaggcaa tgcctgtccg cagaacattt ccttcacgct cgactcgacg
    11221 tcgcccacgg tgacgatcga ccaggccgcc gggcaggaag atccggcgag cgacggcccg
    11281 atcctgttca ccgtcgagtt cagcgaggcg gtgacggggt tcagcggcgc ggatctgagc
    11341 tttgccggct cgaccgcggg gtccgagctc gtcgccgagg tcaccggaag cggctcgact
    11401 tacacggtgt cggtgaccgg aatgagcggt gcgggtacgg tggtcgtgtc cgttccggcc
    11461 gctgcagcgg ccgacgcggc cggtaatacg agcggtgcgt ctaccagcac cgacaacagc
    11521 gtcaccgtcc gcaccaacca ggcgccggtg gcaggagacg acttcttcag ttcgcggccg
    11581 gggcggacgg aggtgatcac gttcgagcag ctgctcgacg acgacaccga cgccgacggc
    11641 gatgcgctgc gggtggtgga tgtggaccgc cagatgctcg acgaggacgg caacgtcttg
    11701 gcggaagtcg agctcgaaga agggcagttg cgagtgaccc cgagcgggct cttcatcggc
    11761 gcgggacggt tcagctaccg catcgcggac gattccggcg gattcgacgc tggcgaggta
    11821 acgatcacct acttcaacca ggcgccggtg gcgggggaag accttttctt cacccgaccc
    11881 gggcagacgg aaacgatcac gttccagcag ctgcttgatg acgacacgga tgccgacggc
    11941 gacgcgctac gcgtggtgga tgtgggccgc gtcgtgctcg acgcgatggg caacgtggtg
    12001 gccgaagtga tagaggtgaa cttcgagcag gggacgctgg tcgtggctcc cgcggagctc
    12061 ttcaccggcg cggggcactt cagctaccgc atcacggacg atttcggcgg cgtcgacgat
    12121 ggggacgtga cgataaccta cgtcaaccag gaacctgtgg cgggggatga cgccttcagc
    12181 tcgcggcccg ggcagacgga agtgatcacg ttcgagcagt tgctcgacga cgatatggat
    12241 gccgacggcg atgcgctgcg ggtggcggat gtggaccgtc aggtgctcga cgagaacggc
    12301 aacgtcgtgg cggaggtgga gctcgaagaa gggcagctac gggtgactcc gaccgggctc
    12361 ttcgtcggcg cggggcggtt cagctaccgc atcggggacg gccacggcgg cttcgaccag
    12421 ggcgaagcga ccatcaccta cgccaaccag gcgccggtgg cgggggagga cctcttcttc
    12481 tggcgacccg ggcagacgga agtgatcacg ttcgagcagc tgctcgacga cgataccgat
    12541 gccgacggcg atgcgctgcg gctggtggac gtggaccgag tcgtgcgtga cgcgatgggc
    12601 aacgtggtgg ccgaagtggt ggaggtgaac ttcgagcagg ggacgctggt cgtggctccc
    12661 gcggagttct tcaccggcgc tgcacacctc agctaccgca tcacggacga tttcgggggc
    12721 atcgacgacg gcgacgtgac gatcacctat atcgacaacg atccgccggt gacggacgaa
    12781 gccttcatcg agctgcctgc cggggaagtc ggcgtcatca cgtttgcgca gctgctggag
    12841 ggcgatttcg atccgaacgg cgatgtgctg cgggtggagt cggcgggcag ccgggtgatg
    12901 gacgtgatgg ggaacgtggt ggccacggtg gagatggact tcgaacaggg acggctggtg
    12961 gtgacgccga ctacgttctt caccggggca ggacggttca attaccgcat cgcggacggc
    13021 aacggcggct tcgacgacgg cgaagtcacc ctgatcttca ccgggaccgc gcccgaggag
    13081 actcaagcgg gcgtaacgct tctcggcgac tcgccggagc tgcagtcgat cttcgatgcc
    13141 agcctcgccg cgtggagcag catgctcggc ctggatgccg cagtggtgca ggagctggac
    13201 gcgatgccgg tcgaggtcgt cgacctcgtg gatggatcgc tggcatatac cgacggcacc
    13261 acgatcttca tcgacgcgga cgccgccggg catggctggt tcgtcgatgc gacgccgctt
    13321 gccagcgagg agtacgagct cgtcgacgtc ggcacgtggc aggcgctcga gttcggccgg
    13381 gccgacgacg cggtggacct cttcaccgtc gtcacgcgcc agctcggcca cctgctcggc
    13441 ttcgaccacg aatcgggctg gaccgtgatg cagccgggca cggtcgcgcc cggcatgcgc
    13501 tataccatcg actggaacgc agccgcggga gcgagcttca gcggcctgcc gccgtcgacg
    13561 ctcaaccgct cgttcggcgc gagcgtcgcc gacttcgtaa gggccgtcac ctagcgcccg
    13621 tttcacaccc ggccgcttgc cgcgggcggg ggtttgataa cttcgtcatc cacgtggacg
    13681 cgaggttatc ccccggggtc ggcatcctcc tgctgctcgc gtgctcgacg ttgttcgctt
    13741 cgaaccacgt cgcggcgcgc atcgctttcg accacggggc gagcgtcgcc gcggcggtcg
    13801 cgctgcgcgc cgctggcaca gccctggtcc tgttttgcat gttgcgcgtg cagggaattc
    13861 cgctgacgct gccgcgcggg ctgcgcgggc cggcgctggt ggccggtgtg ctggtcgcag
    13921 tgcagagcta ctgccttttc tccgccgtgg cgctgattcc ggcggcgctc gcgctgctcg
    13981 tgttccagac cagcgtgctg ctctacgtcc tgatctgctg ggcgagcggc cgggagacac
    14041 cgcgcccgag gtcgctcgcc gccatggcgc tggcgctcat cggccttgcg ctagtgctag
    14101 acgtgaggcc ggcccacttc gccgccagtt gggcgcagct cggtgccggc gtcctgtggg
    14161 cgttcggcgg cgcggtcagc ttcgcgttcg tctactacgc gaatagctac gtgctcaagc
    14221 cgctggacgg ccggctgcgc gcactggtga tgacttccgt gactacggtt ctcgcggtgg
    14281 cgggcggtgc cgcagctgat gcgctggcgg tgcccaggga cggcatcggc tgggtcgggc
    14341 tcgcgctggt gacggtgttc tactgcatcg cgctcagcct gctgttcgtc gtgctgccga
    14401 agctgccgcc cacgagctcg gtggcgctca acttcgagcc gatcgcgctg ctcgcgctcg
    14461 cctgggtctt cctcggccaa gcggtgacgt cggcgcagat cgcgggcgcg ttcgtcaccg
    14521 tgggcgcgat cgcctggatg gggctgatga agaagtagct caggccccgg cgggcttggc
    14581 ggcgagcggt cctttgccga agtagtgggc gtaggccgcg cgctggtcga cctggatgtc
    14641 gttgtgccag aacatgacgc ggcgcgccca ggctttctgc gactcgatca ccttggcgaa
    14701 cagcgggttc tccgccgccc ccttcgcgat caccccgtcc caggccttga gctgggcgtt
    14761 gaggatctcg cgcggcgtgc ggtaggcgcg caccttgtcc ttggtctgca ggtcgaggta
    14821 cgactgcgag tggcgatgca tgcctttcca ccacatgtcg gcgctcgcgg catggctcgc
    14881 gtgcttgagc agcgccttga gctcctccgg cagcgcttcg tacttcttct tgttgatcat
    14941 gatctcgcag acgttattcg ccatgtgata gctctgctgc aggtagaact tggcgacgtc
    15001 cggaaatccc atgatgcggt cgtcgtaggc cgtggcgaac tcggcggcgt cgatcacgcc
    15061 gcggtcgagc gagggcacga tgtccgcggg cgcgagctgt accgcggtca tgccgagcgc
    15121 gttgtagttg ccgatggcga gccccgaggt gcggatcttc aggcccttca ggtccttgag
    15181 tcccctgatc tctttcttga accagccaaa gggctcgcac ggcaccgggc cgagcaggaa
    15241 gctgaagatg tcgagattgg ccgcggcctg tatctcggcg tagagctccc tgccgccgcc
    15301 atactcgatc cagcccagca tctgcatcgc gtccatgccg aagtccggcc cggcgccgaa
    15361 aaggccgaac gccgtgttct tgccgaacca gaacgccggg atgccatgac cgccgtcgag
    15421 cactcccttg ttgaccgcgt cgatcacctg aaggccggga acgaccgcgc cggcgggcag
    15481 catgtcgacc ttcagcctgc cgttggtgat ctcggcgacc ttcttcgtcc agtcgaccgc
    15541 ggcttcgtgg tagatgaact tcaccggcca ggtgctctgg aagcgcagcg tgaccggcgc
    15601 ctgcgcctgg acgatcgccg ggaacccaag cgtcgccgag ccggcggccg ctcccgccag
    15661 gaaacgacgt cgcgacgcgc gcttgttcat gtgtcgtaac cggagctgac ctcttctccc
    15721 gtcttgcggt agaagacgtt cggcttgcgg tagatcgcga ggatcaccgc gccggtgtcc
    15781 gagtgcgttt cgtggaacga tccgggcttg cgccagacgt actcgccggc cttgcagatg
    15841 ccgtcgtggt cgtagaacga gccttcgagc acccaggttt gctcgagctc gggatgcctg
    15901 tgcatcggca ggctggcgcc cggctcccac ttgagcaggc aggtcatctc gcctttggcc
    15961 ttgtcctcgt acagcacctt gatctggatc cccggaaacg ggctcggctt ccagtcgatc
    16021 tggtcgggct tgacgtaggt tgaggcgccg atggtgggct cgatcttcgg cttgcgcttg
    16081 acggcggcgg tcttcacgga catcgcaggc tccttcagag gcgggaaact tcattgtcct
    16141 tttgggaaac gaaaagtcaa taagataatg ggggtatgcg ctttgcgcag ttcatctacg
    16201 acggcgtcga gccggtggaa atggcgatct acggcgtgct gtcgatggcg cggcgcatcg
    16261 cgccccagat cgaaatgttc accgtggcgc cgcgcgcggg ggtggtgcaa ctcgccaacg
    16321 gcctgcgcgt gctcgccgac cacgcgatga gcgcctgccc ggaagccgac gtgctgatcg
    16381 tgtgcggcgg gccgggctgg agccgcgagg cggcgaaccc ggcgacgctc gagttcctgc
    16441 gcacgttccg gccacgccag gcgctggcct cggtctgcac tggcggcatg atcctcgccg
    16501 cgaccgggct gctcgacggc cggcacgcga cgaccaagcg cgacggtttc gaaggcgagc
    16561 cgtcgccgct cgcgctcatg cgccagaagc atcctgcgat caaggcgttg gaggtccgcc
    16621 tggtcgacga gggaaacctg attacaggcg gcggggtgac cctgtgcatc gacgccacgc
    16681 tgcacctgct gaggcgcttc gtcggcgagg acgtggcggt caagactgcc cgagcaatgg
    16741 cctattcgaa cgcctgggaa gccaatcgcc tcgcattcgg cgaatggtca ggcacctgaa
    16801 ggtccgctct tcgatcgggg agcgcatcgc gcggttgcgc gcggatcggc gcatgacgct
    16861 ggacgcgctg gcgcgtgcca ccggcttcac caagagctat ctctcgaaga tcgagaacgc
    16921 gaggaaggtg ccgccgatcg gcagcctgtc gcgcatcgcg gtggcgctcg acagcgacat
    16981 cgccggcttc ttcgacagcg gaccgggcat gcgcgacgcc ggcgcctgcg tggtgcgcgc
    17041 cggcgagcgg cgtccggtgg tgcgcggcgg tacggcgttc ggctacgact acctgagcct
    17101 cgcgtaccgc aagcacgaca agaagatgga gcctttcatc ttcacctttc cggcgaagat
    17161 cgacagccat gtcttcttcg accatgccgg cgaggagttc ctgttcgtac tcaagggccg
    17221 catcgagttc caggtcgggg gcgagcgctg ggtgctgcgc gaaggcgaca gcctgtattt
    17281 cgattcgcac atcccgcacc gcggctggag cctgggcggc gcggccaagg tgctggtggt
    17341 gatctacaag tcggagccca ggaagtgatc aggtcgccgt gatcaagccc tcgccgccgg
    17401 cgtacagctt ctccaccagc gcggcgcgcc gctcgagcac cgcacgctgg ttcatgtagc
    17461 ccttgtcggt aatctcgttg gcgtcgatcg aaggcggctc ggtcatcacc agcaagcgcg
    17521 cggggcgggt cgagctggtg ccctgcatct ttttcagggc gttcgagagc cgcgcgcgga
    17581 tctcctgcgg cgcgagatcc ttcgccgcgg ggctgaggaa cacaagcgcg ccgacttcgc
    17641 tccgatcgtg cccgcaaatc accgcgtcct ggatcagcgg gtcgccggcc gagatcagcg
    17701 cgatgcgcag cgcgccgacg tgcacccagg tgccggtgga gagcttgaag tcctccgcga
    17761 cgcggccgtc gaacacgatg cctttcgccg ggtcggacgg gtcggcgaag cgcacggcgt
    17821 cgccgatgcg atagaagcct tcctcgtcga acgccgcctg ggtgaggtcg gcgcgcttgt
    17881 ggtagccggg cgtgacgttc tgaccgcgca cgcgcacctc gagttttcct gcagccggga
    17941 cgagcttcag ctcgcagccc gcgaccggca ggccgatcac gccggctcgc tccatgggga
    18001 agtgcacaga ggtcgcgagc ggcgaggtct cggtcgaacc ccaggcggac agcatggcaa
    18061 gcttggtatt ttcctcgtct tttgaaaggc gtatcagccg gtcccagagg ttctgaggca
    18121 atgcagcggc cgcgtagaac aacacgtcga ggtcgcggaa gaagttgcgg cgcagctcgg
    18181 cgtccttctc gaggaacggc agcagcaggt cgtagccgcg cggcacgttg aagtacatgg
    18241 tcggcgccac ttccttcagg ttgcgcaccg tggtctcgac cagccccggc gccggcttgc
    18301 cgccgtcgac gtacatcgtg ccgccgttgc gcagcacgag attgaagttg tggttgccgc
    18361 cgaacgtgtg gttccacggc agccagtcga ccacgaccgg cgcgcgctcc tcgacgaacg
    18421 gccaggcctg cgccagcatc tgctggttcg cgcacagcat gcggtgcgtg ttcaccacgc
    18481 ccttcgggat ccctgtcgag ccggaggtga acaggatctt cgcgacggtg tcaggcttga
    18541 ccttggcgaa ttcccgttcc tgggtcgagc ccggattggt ctcgagcagc tcggcgacgg
    18601 aaacggactt cgcgccgacg gcggcgaggg cgggcgcgaa cttctgcgga tcggcggcga
    18661 acaccagccc cggcttcacc agctcgaaga tggtcttcag cttgccgaag tccttcgaca
    18721 tcagcgaata ggcgggcgag accggtgcga ccgggacgcc gacgtgcatg gcgccgagcg
    18781 ccagcaaggc gtggtcgatg ccgttgtccg aaagaatcgc caccggcctc tccgcgttca
    18841 ggtttctatc cagaagtgcc tggccgattc ttcgcaccgc gccgtacgtt tctcggtaag
    18901 tgagcttttt ccagcgatct ccagaacgtt ccgccaggaa ggtcctatcc ggtgcctggt
    18961 cggaccattt gacgagccac tccgtcacac aacgcgcgta aaaaccgagc ttttgcggag
    19021 agcgcagcac tatcgagcca tcccggcggt tctcgacttc gacggcggcg ggtgcgaagc
    19081 gcaatttcga ttgcatgatg cgattctaag tggtcacggg aagcgatgac ctgagacgca
    19141 gccgcttctt acttggtgga gttcacaacc gccggaggat gacctacggg cccacgctgc
    19201 tcgagcagtt cgagcgcgcg tcttgcagcg cgcagaccgg attatcgaat agtcgagcgc
    19261 agcagcgcta gtcgcactgc aggtactctt tgccctgcga ctgcaggacg ggcgagccat
    19321 tcgagctgag cgcaagccgc agcggctgcg gcgaccagct gaagccgtcg cgggagaact
    19381 cgagcgtctc gttctgcacg cgccagcgcg cctgcccacc gggcatctgg ttcaccaggc
    19441 cgttcggcat gaagaccagg cgctccaggc cgcgcttgcc gctgatgtcg taggctgcgg
    19501 cgcaccaggg gctcgcggta agcgcctggc tgagccgctg gctctgcgga gtgctcttgg
    19561 tctcggtttt tgcgagccgc agctcggtcg cccgttgcgg gttcggcttt ccgcccaagt
    19621 ccggctcggt gagcaccatc ttcagctcct cgccaacgag acggcccgtt aggtggagca
    19681 tgccgaagtt gttggccgcg gtgccgcgca ggccgtcggc ctcgacgcgc gcctgcacct
    19741 gcaggctgct tctaccgtgg cccgtaagcg tgccgctcac ccgcttctgg tcttcgtgag
    19801 cgagggtgag catcaaggtg ccccccgaag gattcttcgc ggtgtaggtg ccggcatagt
    19861 tctgtgcgag cgccggggcg caggcgagca gagcggcgga caggcaggcg gcgcgtagca
    19921 tggaagcctc ccactgcaat tttctctatg ctacgcgctg tcccgcgcgg cgcttgcgac
    19981 atgcgtcacg ccggcaaggt cttcaggaag cttttcgaac ctctttactt cccacttggg
    20041 cgctcgcacg agaaaaccga tgcgcgcgcg agagcctttg atcgccaccg ccttgctgta
    20101 gaactcgccg gtggcgtggc tgtagccgac gccggtctca ggagataagg atcggcgata
    20161 atcgtagcca ggatcgagtc gaatcaaggg agcgcgcatg gacaagcaac tgcaggtcgt
    20221 ggatcaggtc acctcggcgg gcatcgacat ggcgatcagg ttcggcccca aggtcctggt
    20281 cgccgcattg atcctggtgg cgggcttcct ggccggccgc tgggtcagcc gcgcgcttgg
    20341 gcgcgcgctc gcgaagttcc agctcgagcc gccggtgctc gcgctgatcg cgcgcgtcac
    20401 gcacctgctg gtgctggggc tgttcgtcat catggctctg caaaacctcg gcgtggagct
    20461 gcttcccttg atcgccggcg tgggcatcgc cggcgcaggc atcgcgctcg cgatgcaggg
    20521 ggtgctcggc aacgtcgtcg ccgggctcac gatcatcttc acgcgccctt tcagggtcgg
    20581 cgactacatc tcgatcgtga aagaggaggg cgaggtcctg gagatccgct tgttcagcac
    20641 cacgctcggg cacgcggacc tctccaaggt ggtgattccg aaccgcaaga tcgtcggcga
    20701 aatcctgcac aactacggcc gggtccgcca gttgccgatc gaagtcgggg tttcctacga
    20761 aaccgacatc ggcgcggcgc tcaagctcgc cgaggagatc ctgcgcgcca acccgcgcgt
    20821 gctgaaggac ccggcgccgg tcaccaaggt gctgaagctg gcggactcga gcgtggtcat
    20881 cgccgctgcg ccctgggtcg cggtgcccga ctacggagcc gcaagcggcg agctgcacca
    20941 ggcaattctc gaggggttcc gccggcgaaa catcgtgatt cctttgccgc agcgagagat
    21001 ccgcatcgct tcgagcagcg cctacgtggc ggcgccggga gcggtcggcc ggctcgcggg
    21061 ccgcgacgcg gcctaggaac actgaaaaag tggcaagtgg tcgttcccgc gaaggcggga
    21121 acccattctt ctcaaaaaaa gaaaactggg cccc
//
LOCUS       DATBSZ010000141        32602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_20463, whole genome shotgun sequence.
ACCESSION   DATBSZ010000141 DATBSZ010000000
VERSION     DATBSZ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 32602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32602
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20463"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VIG70_09355"
     CDS             <1..786
                     /locus_tag="VIG70_09355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEY8250801.1"
                     /translation="GAPLTRLPLLAPDDEPRAPSYDSLYAPTEVASAPLDLRALSRLL
                     EYSLALSAWKQAGGTRWALRANPSSGNLHPTEGYVLAGGIEGLGEVPGVFHYAPREHA
                     LERRADCPQVLFERLTRDLPSQALLVGFSSVYWREAWKYGERAFRYCQHDAGHAIGAL
                     RIAAATLGWSALVLDEVADETIDGLLGLDRAGDFDGAERESAELVMAVWPGQAATGTS
                     NLDVEAVQELKRQRWYGKANRLSLDDPVPYEAIDHVAAASRKL"
     gene            783..1451
                     /locus_tag="VIG70_09360"
     CDS             783..1451
                     /locus_tag="VIG70_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEY8250802.1"
                     /translation="MISAAQIIRQRRSLLACDGKTSIPAQRFYRMLSRAMPAPGDAIP
                     WSPAIHFALFVHRVEGIEPGLYALARDPGKVEKLKQSMHAHFAWEAPPDRPADLPLYL
                     LERGDARELATQVSCRQEIAGEGAFSLGMIAEYQASLVAHGPSFYRRLFWEAGAIGQV
                     LYLEAEAAGVRATGIGCFFDDPVHQVFGFSDLAFQSLYHFTVGGYVDDPRLTTLAPYA
                     RERS"
     gene            1620..1823
                     /locus_tag="VIG70_09365"
     CDS             1620..1823
                     /locus_tag="VIG70_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY8250803.1"
                     /translation="MATGTVKWFNDAKGFGFITPDDGGKDLFAHHSSIQGAGFKSLKD
                     GQKVSFDVTQGQKGPAAANIKPL"
     gene            1885..3453
                     /locus_tag="VIG70_09370"
     CDS             1885..3453
                     /locus_tag="VIG70_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEY8250804.1"
                     /translation="MIVASGIAMQFGAKPLFSNVSVKFGGGNRYGLIGANGCGKSTFM
                     KILGGELEPSAGTVAIDAHERVGRLRQDQFAYEAERVLDVVLMGHAEMWAAMRERDAI
                     YANPQAGEQEFLRAAELEAKYAEYGGYTAEARAGELLSGLGIPTDRHQGFMSAVAPGW
                     KLRVLLAQALFGDPDILLLDEPTNNLDINSIRWLEDVLNRRTSTMVIISHDRHFLSSV
                     CTHMADVDYGEIRVYPGSYDDYMEASTLAKQQLQKDNAKAKDRIADLEEFVRRFRAHK
                     AKARQATSRMKQIEKLKVEDVKPSSRQYPFIRFLVDPKEKLHRLAVEAKGLFYGKLDL
                     SIEAGEKVAIVGPNGAGKTTLLQNLLKAKWAEKANVGYWPQDASTELGFEETLAEWLS
                     RWRRADLKQDDQIVRATLGQLLFSGDDQKKSLKVISGGEAQRMIIGRLVLGMPNAMLL
                     DEPTNHLDMESIESLNTALDKYPGTLVFVSHDREFVSSLATRVIELKDGKAVDFRGGY
                     EEYLLTQTPELAAA"
     gene            3489..3929
                     /locus_tag="VIG70_09375"
     CDS             3489..3929
                     /locus_tag="VIG70_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250805.1"
                     /translation="MKVVVFLALVMPAVAAVAQPRDPCLETYNDEVVAIQREAKAKQN
                     VGNDAAKQRAARGAETQLAAAAKRAKQCQDEAKAPADPTKAPAKAAATAPSTDDCKAR
                     TSDRAADIERRFGSTTLDPAQQAARREEETRLQVELNECNRRRR"
     gene            4065..5825
                     /locus_tag="VIG70_09380"
     CDS             4065..5825
                     /locus_tag="VIG70_09380"
                     /inference="COORDINATES: protein motif:HMM:NF020683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase early set domain-containing
                     protein"
                     /protein_id="HEY8250806.1"
                     /translation="MKKQIGAFLVSLVLVGAASAQQAVDSWAPAGSQAPIELRVNDPV
                     RGDIPFQPVHATLLPDGRVMLFAPVNVHARAAWFVPTPIGDPLPPVLRLTADHVPVNI
                     NTPIDFTDAQGRQWHFEETIFCSGHSLTEDGSIFVAGGTFYYSMRQPNGISVSWIYGM
                     PNTTLYSYPVSTWFRLQPDMIGTGATGSNRRWYGTVTRLSDARMLVSSGFELADTRLQ
                     VPGLPVSHHGTNINRSVEVWTPATPPAASSRSLVSAHLQTPEEVWNPDYTHAFQVPYG
                     PPMIPSNTVLMFGAAGVPVYLLADAPPGTGWITLGNQRRPGTVAADAPNHGTASVMLP
                     MRANNFEWGYANGSVLQAGGGLGSQMERSIDFFELSSGTWAQGIDLGVRRRFPATVLL
                     PDGKVMVVSGYDATNVNPVIRNAHYLDLRPPISPATFTTGAAVSGEVRGYHNIALLLP
                     DGRVLIGGGRSRGSDSGDPEDEKPNFRYLLPPYLASGQARPAITAAPQTIGYGTSFTV
                     EFSGGPISEVVLMGLGSMTHAIDMNQRYLQLATPVKGTTSAEVTGPENIQAAPPGYYM
                     LFVLNESRIPSIARFVRVVP"
     gene            5908..6549
                     /locus_tag="VIG70_09385"
     CDS             5908..6549
                     /locus_tag="VIG70_09385"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HEY8250807.1"
                     /translation="MDSGRSEHLERRLAPAAQPGRLPWPPAVHAILWLDAVLVVAAVL
                     ALVGMPPALGDAPSAWTWIALAGSTLTAVIAALSAFELSAPGRSYAWMLLPAPAIVLW
                     IAGSGMGCLAVPGDADTYGETVAEAGRCLGFLLMISAPLFTLILFMLWRAAQPMPGRV
                     LAMGAVASAGAAASLLALVHPHDSSVLDLGAHAAAFAVVLGASAVAARIRSAA"
     gene            6628..7242
                     /locus_tag="VIG70_09390"
     CDS             6628..7242
                     /locus_tag="VIG70_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8250808.1"
                     /translation="MLTLFYAPGACSMAPHIALEEGGEKYEAKRMDLSKGEQKTADYM
                     KIHPLGRVPALKLDDGTPLTENTAILPFLGKRFGLWPTDPVKEAQALSVIGFFAASVH
                     PANAHVGRPERYTDDKSAHPGLQAMGKKTYFEYMKQIDGMYARREWLSDKYSVLDPYA
                     FVFYVWGVRREMPMAELKNYSAFKDRMLKRPAVQRVAADEGIKL"
     gene            7292..7834
                     /locus_tag="VIG70_09395"
     CDS             7292..7834
                     /locus_tag="VIG70_09395"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY8250809.1"
                     /translation="MERFLRDLVAGLVFAAFGAAAIHFVRDYPLGTAVRMGSGYFPAA
                     LGWILIAFGAFLGVRGLWRWVPSPRKAKQVDGLDFVKGERAPAPAPAAAWDWREATWS
                     VRPVACIAASMLVFGFVMPRLGLIPALVPMFFVAALGGREFRWREVLILTLVMTAFAV
                     GVFVMLLKLPFQLFPGFYLV"
     gene            7827..9302
                     /locus_tag="VIG70_09400"
     CDS             7827..9302
                     /locus_tag="VIG70_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY8250810.1"
                     /translation="MFEGLALGFAVAASVENLLYCLVGVALGTLIGVLPGITPLVAIG
                     MLFPLTFTLPPIPSLIMLAGIYYGAQYGGSTTAILVNLPGETASAVTCIDGYPMARQG
                     RAGPALAIAALASFFAGCVGTLLIAFVGPPLGEWALQFGPWEYCALMLMGLVASAVLA
                     RGDAVKGLAMVALGLLFGVVGTDVNSGVVRYAFDVPELFDGIGFTVIAVGLFAVAEIA
                     SNLESGERREALAAAFSELLPSRHDLRDSSWPMMRGTAVGAVLGVLPGTGPALASFAA
                     YMLEKKVATDPDRFGAGAVEGVAAPEAANNAAAQTGFIPTLTLGIPGSAVMTLMVGAM
                     MMHGFAPGPQAMAEKPELFWGIVASMWLGNLMLLVLNLPLIGLWVSLLKVPYRWLFPS
                     IVMFCCIGVYSVSNNPVDVYLCAALGALGFLLARVGCEPAPLLLGYVLGPLFEEHLRR
                     AMLLGRGDPLLFLTRPISLAFMLGTALVLVAMVWPALRRKQ"
     gene            9384..10097
                     /locus_tag="VIG70_09405"
     CDS             9384..10097
                     /locus_tag="VIG70_09405"
                     /inference="COORDINATES: protein motif:HMM:NF018967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HEY8250811.1"
                     /translation="MSDIATRLGTTTSGQLLVRSGIEIERVLESIIRDQAPVTAKLKD
                     LMFLSRLVHYDPAEQQVTVAYCDHKPSNSAMLSLRSVTFMCNHRGAQLAFACTKPVQS
                     MHLGRPAIRMTAPNILLAMQHRRNQAHGPLPGEADVQVELSMGVISFEAKLVDMSLDG
                     KAFLLGDPGIPLCAGTRLHKARIRDVAREPLLVDIDVDNVRQAVLHNGQRATRIGCRI
                     VAEREKMEKIIRLFIIDLQ"
     gene            10147..11106
                     /locus_tag="VIG70_09410"
     CDS             10147..11106
                     /locus_tag="VIG70_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250812.1"
                     /translation="MRMRLLVMVLLLAFGTSGYAAYPEKPIRFVLPSAAGGSVDVLMR
                     ILAQQLSAQMNVPFVVENKPGASFVLGTMDIVKAPPDGYTLGYGNLVSLAINKSLLGS
                     IPYDVEKDLTLVSNCVRVFNLLAVNNKLPVSTVQELVAYAKKNPGKLTTGSSGNGTTG
                     HLGAELFKAMTGTLIVHVPYKGSPQAINDLMAGQIDMMFDNISSIGPHAKAGRVKGIA
                     VSGPRRSPVFPDLPTVAEAGVPGYETVAWGGVIGPARLPSEVVVRLVREIKTALAAPN
                     VVERYKQLDTETDGGTPEEFRALVRRETPKWAEVIRRAGAKVD"
     gene            11099..12535
                     /locus_tag="VIG70_09415"
     CDS             11099..12535
                     /locus_tag="VIG70_09415"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEY8250813.1"
                     /translation="MTDQFDLVIRNATVIDGTRAPRFQADIAVKAGKIARIGKVKQKG
                     RKEIDAAGKIAAPGFIDAHTHDDRLMLSSPDMAPKVSQGVTTVVAGNCGISLAPAPNG
                     MRPPVPVPMDLLDDEGGWFRFRTFKEYVQALKKKPAATNCALLVGHSLLRVQTMDDTS
                     RTAARAEVKKMQDMVDEAMKAGAIGVSTGLYYEPARAALPEEVIEICRPLAKHGGIYC
                     THMRDEGDRVVDSLEESFRTGRELGVPVVISHHKVAGKANHGRSAETLPIIERAMRSQ
                     KIGLDCYPYHASSTILSASRAANATKVLVTWSKPHPEAAGMELGEIKKTLGLSVYELL
                     PAGAIYFSMDENDVQRILAFEQTMIGSDGLPHDAAPHPRLWGTFPRVLGHYSRDLNLF
                     PLETAVHKMTGLTAKTFGLADRGVLKPDHAADITIFDPKVDETATFAKPIQEAKGIET
                     VIVNGKIVWREGKPTGARPGKVLQRSAR"
     gene            complement(12517..13539)
                     /locus_tag="VIG70_09420"
     CDS             complement(12517..13539)
                     /locus_tag="VIG70_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8250814.1"
                     /translation="MNAHFNGLTKQVHYLGALVHESRIARALEEGVARYPIYRVLRFA
                     ARVELEVLLDAIALDAAWRAERLHAESMLLDADGLFVAAYGNRKADYCSCSFYIWARN
                     LARAEEAKERILAKAGSTRITEPMFSIDWHFLTARGELESATIEEMADDVLRDRAYPA
                     IQGGVHAFVARYLDAPETVLVLQGPPGTGKTRLIRAILGEMSRRKGEQAQALYTGDMK
                     ALTSDEVFVKFITGWHDAFIVEDADHVLKPRADGNEHLHRFLTIADGVVRSQGRKIIF
                     STNLPNVGDLDEALVRPGRCFARIHVRNLTAPEARALAEELGSPAFAVEEGRRHSLAE
                     IYRALR"
     gene            complement(13870..14232)
                     /locus_tag="VIG70_09425"
     CDS             complement(13870..14232)
                     /locus_tag="VIG70_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250815.1"
                     /translation="MRIRAPLLLCLVVGCTMTLDESQLEANIVRPGNFKAGSGVIQSV
                     GVLPGAREPGTGADAKGKYPDRNLYRLYLRMDAGGFQTVDIDNSRFLAGEVVELTNDG
                     RVERISGTTLNEAVGHER"
     gene            14526..15029
                     /locus_tag="VIG70_09430"
     CDS             14526..15029
                     /locus_tag="VIG70_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8250816.1"
                     /translation="MKRRSVRSKTKARKVKPVPAGYHTVTPYLACGDAARAIDFYKKA
                     FGAAEVMRMPGPDGKIAHAEVKIGGSRVMLTDEYREMDFLSPLTRGGTTVHMHLYVAD
                     VDKMADRAVGAGAKLLHPVQDKFYGDRTGTVRDPFGHVWHIATHTEDLSMAELRKRGE
                     KALKEIG"
     gene            complement(15026..15295)
                     /locus_tag="VIG70_09435"
     CDS             complement(15026..15295)
                     /locus_tag="VIG70_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250817.1"
                     /translation="MELVLWAAAGAAIGWLAITKLGLNEDRGTLVSIIIGAAGGVAGG
                     KLVAPMFGAVAAVPGEFSMMALVVVLATSAACLTIGNMVSNRFGV"
     gene            complement(15297..15890)
                     /locus_tag="VIG70_09440"
     CDS             complement(15297..15890)
                     /locus_tag="VIG70_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8250818.1"
                     /translation="MSKRAKNEGSARERVFRTAAALFHRHGIRAVGVETIAAEANTTK
                     MSLYRNFPSKDELVAAWLRDHDARFWQSWDAMSREYPDDPRRQLKAAFELLAKHVADP
                     KARGCPMANAAVEITESDHPATKVIKVHKARLRARLAELCARIGVGDSRLLADQLFLL
                     MEGAQVSAQTPGARAAAASVARAAAVLIDSNRFSTEV"
     gene            16032..16247
                     /locus_tag="VIG70_09445"
     CDS             16032..16247
                     /locus_tag="VIG70_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250819.1"
                     /translation="MMAAIVTTMLFLPAAVLLAGLLFLVGASLQAFATFNGVLNAYLG
                     VAAWWIIAFLPTLAYSVYVMPWERREP"
     gene            16244..16495
                     /locus_tag="VIG70_09450"
     CDS             16244..16495
                     /locus_tag="VIG70_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250820.1"
                     /translation="MKSSDVPRMALRRAVEWCGGIDAVGDALNVDGERLKKWIAGEQE
                     VPQAVFVQAVGLILEAAATQSPRGRTPDSNDGGRKARRH"
     gene            16521..16724
                     /locus_tag="VIG70_09455"
     CDS             16521..16724
                     /locus_tag="VIG70_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEY8250821.1"
                     /translation="MAAGKVRWFSDEKGFGFITPDDGGADLFAHHTALSGDACQSLKQ
                     HQRVEFELRPGTRGPRAANIRAL"
     gene            complement(16711..18012)
                     /locus_tag="VIG70_09460"
     CDS             complement(16711..18012)
                     /locus_tag="VIG70_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8250822.1"
                     /translation="MDPRHGGSFSLSATEAARRIERGELTSEALVSACLERIAERDER
                     LRAWAFVDRDYALAQARALDRGPRRGPLHGIPFGIKDAIDTAELPTEYNSPIYRGHRP
                     KADAACVVALKRAGAVILGKTVTTEFANNHPAQTRNPHNPAHTPGGSSSGSAAAVADF
                     MVPAAIGTQTGGSVIRPAAYCGVAAIKPSFGAINRAGLKMVAESLDTIGVFAREPEDL
                     AFALEALCGRAVPDFPSFEKPRIGLCRTPRWELADAATQANLEAAAQRLALAGAKVSD
                     YQMSLESRQLFDRHKVIMGYETARALAWEYGAHPDKISASLKPRLDEGWKVTRADYDA
                     MRALAERCRRALAEAMRDFDFLLTPSASSEAPASLASTGDPVFNRAWTLLGVPCVTLP
                     HGRGPAGLPLGMQLVGAFDGDAALLGWAKWAQRALGGATAP"
     gene            complement(17969..19327)
                     /locus_tag="VIG70_09465"
     CDS             complement(17969..19327)
                     /locus_tag="VIG70_09465"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8250823.1"
                     /translation="MTTPEDPEGKRLPIKLDTTSNGEFVPVPLSASNRAANRLAHEAA
                     SANSRKLNTSRRTFLVSACGAASTLLAFNQAHGTSGGFYDLDRESALDAQLARVQTGP
                     AKDELIFDVQGHFIDTPKGNPKNHEVFIKDVFMDSDTDLMVLSFVPSTRDSEPVTIQA
                     ADEVRRIVDKLQGTHRLLLHGRVNPNQPGDLEGMDELAQKWGVSAWKTYTQYGPGGKG
                     YFLSDDVGTRFIERARKLGVKVICVHKGLPFGSRSYEHSQCSDIGVVAKRFPDVKFLI
                     YHSGFVSSVKEGPYDASPGRDGIDTLISSLQKNGVSPGSNVYAELGSTWRFLMRDPEQ
                     GAHAMGKLIKHCGEDNVLWGTDSIWFGSPQDQIVAFRTFQISPAFRDRFGYTEITPAV
                     RAKVLGQNGAKVYGISASELKRYTANDAVAREKTAYLENPSPHFLTYGPKTRREFLAF
                     GH"
     gene            complement(19324..19488)
                     /locus_tag="VIG70_09470"
     CDS             complement(19324..19488)
                     /locus_tag="VIG70_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250824.1"
                     /translation="MFGSQRGLGDLLMQAMSLHNIERILALTLRLVAFAAGVNALLLA
                     LDRHLHRALP"
     gene            19799..20053
                     /locus_tag="VIG70_09475"
     CDS             19799..20053
                     /locus_tag="VIG70_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250825.1"
                     /translation="MNPLLQVEEVQDMVNMLFQRYPTLCGFSVKDDMSFSNVACHPAL
                     ASEEAEMLCEEISATLHELMEEAPEAAEMLRGRTVARTFH"
     gene            20050..20634
                     /locus_tag="VIG70_09480"
     CDS             20050..20634
                     /locus_tag="VIG70_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY8250826.1"
                     /translation="MKKLLLAAFLFAAACAARAQDLDRPLLLVAAPDLQGLYSRTALL
                     VVPAAGGQHIGFIINRATDVKLATLFPEHAPSAKVADPVYFGGPEMIGSIFAIVRRDP
                     GAPAMHLFGDLFVTGNGAAVDRIIEKTPNDARYFAGFVGWGMHELAKELKDGFWYVAE
                     PDAGLLFSKDPAGIWEELVKRAGGSPLPAGANSI"
     gene            complement(20664..20969)
                     /locus_tag="VIG70_09485"
     CDS             complement(20664..20969)
                     /locus_tag="VIG70_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250827.1"
                     /translation="MEKPELTTFKIGKYEIRAQPASGSAHMLRYTVLLDGRRLGALLS
                     VPTESDCRFLESPPKVPPLKIFQVTYRPGRPKKGAVRPQEEGEGGPRIPWTIKGQRY"
     gene            21086..21358
                     /locus_tag="VIG70_09490"
     CDS             21086..21358
                     /locus_tag="VIG70_09490"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250828.1"
                     /translation="MEHTQIDKVPSNVQEVPGRGESFQAARERLNQTLSGVSERGLNA
                     AREAAHYADRTVHNSPWTSVGIGFGLGVVVGALIAIAAGSRHSRFG"
     gene            21401..21907
                     /locus_tag="VIG70_09495"
     CDS             21401..21907
                     /locus_tag="VIG70_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEY8250829.1"
                     /translation="MKISMYQASAPRFANALRNLSAILDKAQAHAEAKKLDPAVLGSL
                     RLIADMFPLARQVQVACDTAKGALARLAGAEIPKYEDTEQTIAELKARIARTLDFVAS
                     LPAAKIDGSEEREVVMKMRGQDVKFSGLQYLFGHAWPNFYFHVTTAYNILRANGVELG
                     KRDFIGNP"
     gene            complement(21918..22349)
                     /locus_tag="VIG70_09500"
     CDS             complement(21918..22349)
                     /locus_tag="VIG70_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250830.1"
                     /translation="MRLAVLLTIPIAALLAACAPAPVTKAETEGLDYGPRPVRWKEEI
                     TSYLAVRLTDPKAAIIEFRNEPAQMYQRAVAFRPMHFGWAVCVWVNDKNRSGAFSGFY
                     PMTVFIRHEKIVQVNSGPDDFGVGAQYARSQCEQLGAPFKQ"
     gene            22379..22693
                     /locus_tag="VIG70_09505"
     CDS             22379..22693
                     /locus_tag="VIG70_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250831.1"
                     /translation="MQRWVERPEARWAFWAGMVGAVAMGLLSTYRILSQGSAAAGFAL
                     VFVPLAAAAVAVPAAVWGAALGHVVLHLRGRAGEPRIVFWAALMAAILPPAALAWELW
                     RR"
     gene            complement(22638..23267)
                     /locus_tag="VIG70_09510"
     CDS             complement(22638..23267)
                     /locus_tag="VIG70_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250832.1"
                     /translation="MSISEPTTLVTDYLLAGVTGWLAVLLLRNSESQDSRKSWAAAFA
                     ALALGAFLGGTWHGFFQHAFLWKATVLAVGAASFAMVAGSAIATLSGAARKLVLGLAL
                     IKLLIYGGWMMVRDDFIFVVVDSGAAFAVVAALHLWKWNGFLLGGVALSVVGALIQAS
                     GFSLHPHFNHNDLYHVIQIAAMALLYQGARRLSDARAPTPVPPAAGWRP"
     gene            complement(23264..23644)
                     /locus_tag="VIG70_09515"
     CDS             complement(23264..23644)
                     /locus_tag="VIG70_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250833.1"
                     /translation="MWVLLALFVLRVLGQALVVFLNVGFLPPMDAWYSGLMPYEYLLP
                     SQILIIALMTKICLDFTRRRGFFFEPKKSFATAWLWFGYVYLAAMVARAVLLWDHPIP
                     IVFHWVLAAFVITVGHSHRQRLRR"
     gene            complement(23652..24989)
                     /locus_tag="VIG70_09520"
     CDS             complement(23652..24989)
                     /locus_tag="VIG70_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8250834.1"
                     /translation="MQPDTLDGIRAALRAAQKEERAVCISGARHAMGGQQFLADGLMI
                     DTRRMNRVLGFDPEKGHLEVEAGIQWPQVLQNLVTVQKGRERQWTFAQKQTGADKLTI
                     GGCLAANVHGRGLKMPPFIGDVESFRLLTPKGEVVNCSRTDNAELFRVAIGGYGLFGL
                     ITSVTLRLVERRKLERVVEVRSIDGLPKAFGERIHDGFLYGDFQYAIDETSGDFLNRG
                     VFSCYRPVDPATPIPPGQKELLERDWIELLTLAHHAKGEAFRRYAGHYVASNGHVYWS
                     DEAQMSVYPENYHRALDQRVGAQVRATEAITEIYCERDALERFMMEVREYARRDKVDI
                     IYGTVRLIEQDRESFLAWARRPYACVIFNVHVEHTTSGLIKAGDVFRRLIDIGLKYGG
                     SYYPTYNRYALRRQVDACYPQLPEFLKLKLKYDPQKLFQSEWYRHYERMYEKK"
     gene            complement(25142..25405)
                     /locus_tag="VIG70_09525"
     CDS             complement(25142..25405)
                     /locus_tag="VIG70_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250835.1"
                     /translation="MPALERKQYKLGKYDIVAQPQLGSAHMLRYTVMLNGRRLGALMS
                     VPSESDCRFMEAPPPVPPLRIFALTNRPGRPKKGSTPLRNEEL"
     gene            complement(25440..25757)
                     /locus_tag="VIG70_09530"
     CDS             complement(25440..25757)
                     /locus_tag="VIG70_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250836.1"
                     /translation="MSAPAPSKDAPAKTSASHDPQKAYVELAERIFVQLSSRVYGTLA
                     GSDQKKPDPKALAAFSFKLADAFEQATRETDRMRASIEAANKAAVKLDEVDLSSVFSA
                     TKK"
     gene            complement(25754..27553)
                     /locus_tag="VIG70_09535"
     CDS             complement(25754..27553)
                     /locus_tag="VIG70_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEY8250837.1"
                     /translation="MEHGNDFLVQASLYFAAAVVAVMISHKLGLGSVAGYLLAGIAIG
                     PWGFQLVDTVDHIKAFAELGVVFLLFVIGLELEPKRLWNMRAKLLGLGLSQVLGSIAA
                     LALAGWALGFDLRAALIAAMALSLSSTALALAPLTERGALTTQGGQGTFAVLLFQDIA
                     VIPMLALLPLLGTGAGGAGFSWEKLGLAAAVIVVTLGGGRLVARPVFRHIARTRLREI
                     FTAFALLLVLVIALGFEHAGMSMALGAFLGGVLLAESEYRHEIEAAVEPFKGLLLGLF
                     FIAVGMSADFGVLGARPWLVFGLIAGLFLIKGAVLWLIARWAGIPRPQRALFIVLLAQ
                     GGEFAFVMLGLAVTHGGIGGEIAQAITLAVAISMLLTPFLLALHDQLAPYFAEPAPER
                     APDVPQASNVIVAGLGRVGQVVARMLNAAGFQPTVLDDDPDHVEQSRKFGFRVFYGDA
                     TRLDLLHAAGADSADFLIIALDNAEAITRLAEVARTHFPRLRVIARAHDMRHMFKLRD
                     VGVEAIERETWLSALKLGELAVAHASKDPERARRTAETFAEHDIEVVAKLYEVHRDQP
                     GAHVTVSNELRDQLARTLSEDQRRIAVIKPERS"
     gene            27590..27883
                     /locus_tag="VIG70_09540"
     CDS             27590..27883
                     /locus_tag="VIG70_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250838.1"
                     /translation="MDSVEAELQGRVARLFARRPELWRFSVRDRSMLPDTLDPTSLAG
                     ELFIFEIGLSPRQGKEHYDEVYDDIARAITAAVAARPEARRLLPGRTFVRALH"
     gene            27893..28681
                     /locus_tag="VIG70_09545"
     CDS             27893..28681
                     /locus_tag="VIG70_09545"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEY8250839.1"
                     /translation="MRAGIPYTLATGEKLVNETFGPNNIRRRTSGAQELQPMEIANGR
                     GKATSLDENGFVFVEHKTAVKDFFDPNELEKVYYAEVVALVTRISGANRVQVFDHTLR
                     SGDESEREARLVREPVLSAHNDYTEWSGPNRVRELLPDEADSLLKKRFAIIQVWRAIN
                     RPIQSNPLAIADAKSIAPDDLLIAERRYPNRVGQTYRLKYSPAHRWFYFPEMRRDEAL
                     VFKVYDSEKDGRARFTPHTSFDDPSTPPGAPPRQSIEARALAFF"
     gene            complement(28678..29919)
                     /locus_tag="VIG70_09550"
     CDS             complement(28678..29919)
                     /locus_tag="VIG70_09550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8250840.1"
                     /translation="MADKKIFYGWRMVGAAVGLQFLQAGLVTQAFGAYLAVLAEEQGW
                     SKTALSGAAALQQMEVALLGPVLGWAIDRFGPKVFVRAGVFVFGAGLCLLSQVTSLPT
                     FYGAFIVIALGASLCGYFPLNVALIHWFERKRARALSAMSFGLAAGGIMVPLVAQSLT
                     TFGWRGTALASGLIAIAIGLPLALVIRNSPHEHGEVVDGIPEEAPEKNQTPEAADSTR
                     DFTAREALRTQAFWLISLGHGFALFAVHAVSVHAITHLKEGLGYSVAAASFVISLVTI
                     FQVVGVFLGWAIGDRYKKRLIAAACMLAHALGLLLLTYASNWLMVVAFAVLHGTAWGL
                     RGPFMQAMRADYFGRSAIGMILGLSFMIIVVGQVGGPIIPGIFADMTGDYRVGFTIIA
                     ILAGLGSGFFLLARKPKRPSL"
     gene            29965..30294
                     /locus_tag="VIG70_09555"
     CDS             29965..30294
                     /locus_tag="VIG70_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250841.1"
                     /translation="MKPAQLGALNGDLAPIEEDLATRIEAIFDRCPQLFGFTVQDSSA
                     LPEELRSIALERELVVSDIGVYPYLTADQCEQIYNEIAVALLDFMFERPAAKDVLRGR
                     TFVRTLH"
     gene            complement(30259..31143)
                     /locus_tag="VIG70_09560"
     CDS             complement(30259..31143)
                     /locus_tag="VIG70_09560"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8250842.1"
                     /translation="MAKFVISPHFRLHEWVAEEKGYFREAGLDYEFREVFKSHATPNK
                     VGAYTSFEEGRETNVSCACHWTVNVAASQGLGKMVADCYSVSPGGVFVPAQSPIREPK
                     DLKGVPISVGHQSGSHYSTLQALEQYLPREDIKLSFADGILFGRMEKLIDRQVPAAAL
                     FSGPYYFLEQLGFRKILDTTFMMASMLTGDPDPEDVRKYFRALRRAQRDIDLRPELYT
                     RHYAKEFPARFQGEIDTRRWGPGERIVFEPYTREVFEESFKWIAEREIFAPGTMGAGR
                     FEEACVSLVQGTDERTPA"
     gene            31315..31569
                     /locus_tag="VIG70_09565"
     CDS             31315..31569
                     /locus_tag="VIG70_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250843.1"
                     /translation="MKTDRHVQEQLESLFERCPDLCGFSVREEAQELYVSDIGISPRI
                     SAEQYGEIYQDIAQTLGELLEEEPLAGDWLRGRTFARTLH"
     gene            31641..31964
                     /locus_tag="VIG70_09570"
     CDS             31641..31964
                     /locus_tag="VIG70_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250844.1"
                     /translation="MKTLQQFDSLLGRLEADVDARIRSLFARCPTLFGFAVQDRAMLP
                     KDVDPNRIPDADLFVTDIGIYPKIDSQYDEIHDEITIAISDLVHDQPHAYDYLRGKTF
                     ARSLH"
     gene            32120..32398
                     /locus_tag="VIG70_09575"
     CDS             32120..32398
                     /locus_tag="VIG70_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250845.1"
                     /translation="MGYRIDFRVGGGVLEASGEHVLVDVRGLRDSHGRLRTLFAAKEL
                     PPRVAVIDAWRSDRYCIFTEMAARRLGCDLRRFEDHRTALAWLRSAAA"
     gene            32407..>32602
                     /locus_tag="VIG70_09580"
     CDS             32407..>32602
                     /locus_tag="VIG70_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250846.1"
                     /translation="MSYRIEYSVSGGVLMATLTGTCPTAGFITREISRQARENSASCL
                     LVDVRGLRDRAGRLREVLASR"
BASE COUNT         5422 a        10794 c        11105 g         5281 t
ORIGIN      
        1 ggcgcgccgc tcacgcggct gccgctgctc gcgccggacg acgagccgcg ggccccctcg
       61 tacgacagcc tgtacgcgcc gaccgaggtg gcgagcgcgc cgctggatct gcgcgcgctg
      121 tcgcgcctgc tcgagtactc gctcgcgctc tcggcgtgga agcaggccgg cggcacgcgc
      181 tgggcgctgc gcgcgaatcc gtcgagcggc aacctgcatc ccaccgaggg ctatgtgctc
      241 gccggcggga ttgaggggct cggtgaagtg ccgggcgtgt tccattacgc gccgcgcgag
      301 cacgcgctcg agcgccgcgc cgactgcccg caggtgcttt tcgagcgact gacgagggat
      361 cttccgtcgc aagcattgct ggtggggttt tcctccgtct attggcgcga ggcgtggaag
      421 tacggcgagc gcgccttccg ctactgccag cacgacgcgg gtcatgccat cggcgcgctg
      481 cgcatcgcgg cggcgacgct cggctggtcg gcgctggtgc tcgacgaggt ggccgacgaa
      541 accatcgacg ggctgctcgg cctggaccgg gccggcgact tcgatggtgc cgagcgcgaa
      601 agtgccgagc tggtgatggc ggtatggccg ggtcaggcgg cgacaggaac ttcaaatctt
      661 gatgtcgagg cggtgcagga gctgaaaagg cagcgctggt acggaaaggc gaaccgcctc
      721 agcctcgacg accccgtgcc ttacgaggcg atcgaccacg tcgcggcggc gtcgcgaaag
      781 ctgtgatctc cgccgcacaa atcatccgcc agcggcgcag cctgctcgcc tgcgacggaa
      841 agacttcgat cccggcgcag cgcttctacc ggatgctgtc ccgggcgatg ccggcgccgg
      901 gggacgcgat tccctggagc ccggcgatcc actttgccct gttcgtgcac cgcgtggagg
      961 ggatagagcc gggcctgtac gccctcgcgc gcgaccccgg caaggtggaa aagctcaagc
     1021 aaagcatgca cgcgcacttc gcctgggaag cgccgccgga ccgtcccgcg gacttgccgc
     1081 tctatctgct ggagcgcggt gacgcgcgcg agctcgccac gcaggtgagc tgccgccagg
     1141 aaatcgcggg cgagggcgcg ttttccctcg ggatgatcgc cgagtaccag gcgagcctgg
     1201 tcgcgcatgg gccgtcgttc taccggcgcc tgttctggga ggccggcgcc atcgggcagg
     1261 tgctttatct ggaggcggaa gcggccggcg tgcgcgcgac cggcatcggc tgcttcttcg
     1321 acgacccggt gcaccaggtc ttcggcttca gcgacctcgc cttccagtcg ctgtaccact
     1381 tcaccgtcgg cggctacgtc gacgacccgc gcctcaccac gctcgcgccc tacgcgcgcg
     1441 agcggtccta ggatgtgcta aagtgcgttc cgcaggattt caagtcagcg aatgctcgtc
     1501 tccgaccacc gccaaaagcg gtctaccagt ggcagtcaat tccttcttga tcatcgtgcc
     1561 cgcagcgcag ctcggatagc cgcgcgcaaa gcagtaaatt tttcaggatt gaaatgacaa
     1621 tggctacagg taccgtgaag tggttcaacg acgcgaaggg cttcggattc atcacgccgg
     1681 atgacggcgg caaggatctg ttcgcgcatc acagctcgat tcagggcgcg ggcttcaaat
     1741 cgctgaaaga cggccagaag gtctctttcg acgtgacgca gggccagaag ggcccggcgg
     1801 cggcgaacat caagccgctg taaggagtca ggtcgcagcg caagccggct tcggccggct
     1861 ttttcttttt ctggaaagca ccgagtgatc gtcgcctccg gcattgccat gcagttcggg
     1921 gccaagcccc ttttttcgaa cgtctccgtg aagttcggcg gcggcaaccg ctacggcctg
     1981 atcggcgcca acggctgcgg caagtcgacg ttcatgaaga tcctgggcgg cgagctcgag
     2041 ccgagcgcgg ggacggtggc gatcgacgcc cacgagcggg tcggaaggct gcgccaggac
     2101 cagttcgcct acgaagccga gcgcgtgctc gacgtggtgc tgatgggcca cgccgagatg
     2161 tgggcggcga tgcgcgagcg cgacgcgatc tacgccaacc cgcaagccgg cgagcaggag
     2221 ttcctgcgcg cggcggagct ggaggcgaaa tacgcggagt acggcggcta caccgccgag
     2281 gcgcgcgcgg gcgagctgct gagcggcctg gggattccca ctgatcggca ccagggtttc
     2341 atgagcgcag tggcgccggg ctggaagctg cgggtgctgc tggcgcaggc gctcttcggc
     2401 gacccggaca tcctgctgct cgacgagccg accaacaacc tggacatcaa cagcatccgc
     2461 tggctcgagg acgtgctgaa ccgccgcacc agcaccatgg tgatcatttc ccacgaccgg
     2521 cacttcctca gcagcgtctg cacgcacatg gcggacgtgg actacggcga gatccgcgtc
     2581 tacccgggca gctacgacga ctacatggaa gcgtccacgc tcgccaagca gcagctgcag
     2641 aaggacaacg cgaaggcgaa ggaccgcatc gccgacctcg aggagttcgt gcggcgcttc
     2701 cgcgcccaca aggccaaggc gcgccaggcc acctcgcgca tgaagcagat cgagaagctc
     2761 aaggtggagg acgtaaagcc ctccagccgg cagtacccgt tcatccgctt cctggtcgac
     2821 ccgaaggaga agctgcaccg gctcgcggtg gaggcgaagg gcctcttcta cggcaagctc
     2881 gacctgtcga tcgaggcggg cgagaaggtg gcgatcgtcg ggcccaatgg cgccggcaag
     2941 accacgcttt tacagaatct gctcaaggcc aaatgggcgg agaaagcgaa cgtcggctac
     3001 tggccgcagg atgcttcgac cgagcttggt tttgaagaaa cgctggccga gtggctgagc
     3061 cgttggcgcc gcgcggatct gaagcaggac gaccagatcg tgcgcgcgac cctcggccag
     3121 cttctcttct cgggcgacga ccagaagaaa tcgctcaagg tgatctccgg cggcgaggcg
     3181 cagcgcatga tcatcggcag gctggtgctc ggcatgccca acgcgatgct gctcgacgag
     3241 ccgaccaacc acctcgacat ggagtcgatc gagtcgctca acaccgcgct cgacaagtac
     3301 cccgggacgc tggtcttcgt ctcgcacgac cgcgagttcg tctcctcgct cgccacccgc
     3361 gtgatcgagt tgaaagacgg caaagcagtg gacttccgtg gagggtacga ggaatacctg
     3421 ctcacccaga cgccggagct cgcagcagcc tagcccggcc agcaggagag ggcggtagac
     3481 tggcggcggt gaaggtcgtt gttttccttg ctctcgtgat gcccgcagtc gccgccgtcg
     3541 cgcagccgcg tgatccctgc ctggagacct acaacgacga ggtcgttgcc atccagcgcg
     3601 aagccaaggc gaagcagaac gtcggcaacg acgcggcgaa gcaacgcgcc gcgcgcggcg
     3661 ccgagaccca gctcgcagcc gcggcaaagc gggcgaagca gtgccaggac gaagccaagg
     3721 cgcccgccga cccgacgaag gcacccgcaa aggcggcggc aacggcgccg tcgaccgacg
     3781 actgcaaagc gaggacgagc gaccgtgccg ccgacatcga gcggcgcttc ggcagcacca
     3841 cgctcgatcc ggcgcagcag gccgcgcggc gcgaggagga aacccgcctc caggtcgagc
     3901 tcaacgagtg caaccggcgc cgtcgctgag gcgcgcccgc cgtggtgaat cgggtccacg
     3961 gtccaactcc ccggagcatg ggatagccga tagccccggt tcattgcagt gtcacggatt
     4021 ggtgcgcaca atcgcatcaa gaacggtccc gccgggaggt caccatgaag aagcagatcg
     4081 gcgcttttct agtctctctg gttctagttg gagcggccag cgcgcagcaa gcggtcgaca
     4141 gttgggcgcc ggcggggtcg caggcgccga tcgagctgcg ggtcaacgac ccggtccgcg
     4201 gggacattcc cttccagccg gtgcacgcga cgctgctgcc cgatggccgc gtgatgctct
     4261 tcgcccccgt caacgtgcac gcgcgggcgg cgtggttcgt gccgaccccg atcggggatc
     4321 cgctcccgcc cgtgctccgg ctgaccgccg accacgtgcc ggtcaatatc aatacgccga
     4381 tcgacttcac cgacgcgcag ggccggcagt ggcacttcga ggagacgatc ttctgctcgg
     4441 ggcattcgct gacggaagac ggcagcatct tcgtggccgg cggaacgttc tactactcga
     4501 tgcgtcagcc gaacgggatc agcgtcagct ggatctacgg tatgccgaac accacgctgt
     4561 actcgtaccc ggtgagcacc tggttccgcc tccagccgga catgatcggc accggcgcca
     4621 ccggcagcaa ccggcgctgg tacggcacgg tcacgcgcct gtcggatgcg cgcatgctgg
     4681 tcagctccgg attcgagctc gccgacaccc ggctgcaggt gccggggctg ccggtgtcgc
     4741 accatggcac gaacatcaac cgcagcgtcg aggtctggac gccggcgacg ccgcccgcgg
     4801 ccagctcgag gagcctcgtg tcggcgcacc tccagacgcc cgaggaggta tggaaccccg
     4861 actacacgca cgcgttccag gttccgtacg gtcccccgat gatcccgtcg aacaccgtcc
     4921 tcatgttcgg agccgcgggc gtgccggtgt acctcctggc cgatgcgccg ccgggaaccg
     4981 gatggatcac gctcggcaac cagcggcgtc ccggaacggt cgccgcggac gcgccgaacc
     5041 acggcacggc gtcggtgatg ctgccgatgc gggcgaacaa cttcgagtgg ggctacgcga
     5101 acggcagcgt gctgcaggcg ggcggcgggc tcgggtcgca aatggagcgc agcatcgatt
     5161 tcttcgagct ctcgagcggc acctgggcgc agggcatcga cctcggcgtg cgccggcgct
     5221 tcccggcgac cgtgctcctg cccgacggca aggtgatggt ggtctcgggc tacgacgcca
     5281 ccaacgtcaa cccggtgatc cgcaacgcgc actacctcga cctgcggccg ccgatctcgc
     5341 ccgccacgtt caccaccggc gccgccgtct ccggcgaggt gcgcgggtat cacaacatcg
     5401 cgctgctcct cccggacggc cgcgtcctca tcggcggcgg ccgcagcagg ggatcggact
     5461 ccggcgaccc ggaagacgag aagccgaact tccgctacct cttgccgccg tatcttgcgt
     5521 ctggccaggc aaggcccgca atcaccgccg cgccgcagac catcggttac ggcacgagct
     5581 tcacggtgga attctcgggc gggccgatca gcgaggtggt gctgatgggc ctgggctcga
     5641 tgacgcacgc catcgacatg aaccagcgct acttgcagct cgccactccg gtgaagggca
     5701 ccacctcggc ggaggtcacc gggccggaga acatccaggc cgcgccgccg gggtactaca
     5761 tgctgttcgt gctcaacgag agccgtatcc cgtcgatcgc gaggttcgtg cgcgtcgtgc
     5821 catagcgcag ctgcgcgtcg accgaaaatt tgaaaatctc tgcaaattga cgccggaaga
     5881 agagcccgcg gggggatcat gccggccatg gattccggcc ggtccgagca tctggagcgc
     5941 aggctcgccc ccgcggcgca gccgggacgg ctgccgtggc cgccggcggt tcacgcgatc
     6001 ctctggctcg acgcagtgct ggtcgtcgcg gcggtgcttg cgctcgtcgg catgcctcca
     6061 gcgctcggcg atgcgcctag cgcgtggact tggatcgcgc tcgcgggctc gacgctcacc
     6121 gcggtgattg cggcgctttc ggccttcgag ctgagcgcgc cgggccgcag ctatgcctgg
     6181 atgctgctgc cggcgccggc catcgtcctg tggatcgccg ggagcggcat gggttgcctg
     6241 gcggtcccgg gcgacgcgga cacgtacggc gaaacggttg ccgaggcggg gcgctgcctc
     6301 gggttcctgc tcatgatctc ggctccgctg ttcaccctca ttctcttcat gctgtggcgc
     6361 gctgcgcagc ccatgccggg gcgcgtgctc gcgatggggg ccgtcgcctc ggcgggcgcc
     6421 gccgcgagcc tgctcgctct cgtgcacccg cacgattcat cggtgctcga tctgggcgcg
     6481 cacgcggctg cgttcgccgt cgtcctggga gccagcgccg tcgcggcgcg catccgatca
     6541 gccgcgtagc gagccggatt gtccggagag cctcgcaccg ggattagaat cgccgctcct
     6601 accgtacccc ttgcgaggag caccccgatg ctgacgctgt tctacgcccc cggcgcctgt
     6661 tcgatggccc cccacatcgc gctcgaggag ggcggcgaga aatacgaagc gaagcgcatg
     6721 gacctctcga agggcgagca gaagaccgcc gattacatga agatccatcc gctcggccgc
     6781 gtgcccgcgc tcaagctcga cgacggcacg ccgctcaccg agaacaccgc gatcctgccg
     6841 ttcctcggca agcgcttcgg cctgtggccg acggacccgg tgaaggaggc gcaggcgctc
     6901 tcggtcatcg gctttttcgc cgcgagcgtg catccggcga atgcgcacgt cggccgcccc
     6961 gagcgctaca ccgacgacaa gtcggcccat cccggtctcc aggccatggg caagaagacc
     7021 tatttcgagt acatgaagca gatcgacggc atgtacgctc ggcgcgaatg gctgtccgac
     7081 aagtactcgg tgctcgatcc ctatgccttc gtgttctacg tctggggcgt gcggcgcgag
     7141 atgccgatgg cggagctcaa gaactacagc gcgttcaagg accgcatgct gaagcgcccc
     7201 gcggtgcagc gcgtcgccgc ggacgaggga atcaagcttt agcagttcgg cgtagactgc
     7261 gcggcgtccg ctgctacgcc gcggttcgcg catggaacgc tttctcaggg acctggttgc
     7321 cgggctggtg ttcgccgcgt tcggcgcggc ggcgatccac ttcgtgcgcg actatcctct
     7381 cggcacggcg gtgcgcatgg gctcgggcta tttcccggcc gcgctcggct ggatcctgat
     7441 tgcattcggc gcgtttctcg gtgtgcgcgg cctctggcgc tgggtgccgt cgccgcgcaa
     7501 ggcgaagcag gtcgacgggc tcgacttcgt caagggcgag cgcgcgccgg cgcccgcgcc
     7561 ggcggcggcg tgggactggc gcgaggctac gtggagcgtg cggcccgtgg cgtgcatcgc
     7621 ggcctcgatg ctggtgttcg gcttcgtcat gccgcggctc gggctcattc ccgcgctggt
     7681 gccgatgttc ttcgtcgcgg cgctcggcgg gcgggagttc cgctggcgcg aagtgctcat
     7741 tctgaccctc gtgatgacgg cgttcgcggt gggcgtgttc gtgatgctgc tgaagcttcc
     7801 ctttcagctg ttcccggggt tctatcttgt ttgagggcct tgccctcggg ttcgcggtcg
     7861 ccgcttcggt cgagaacctg ctctactgcc tcgtcggcgt ggcgctcggc acgctgatcg
     7921 gcgtgctgcc gggaatcacg ccgctcgtcg cgatcgggat gctgtttccg ctcacgttca
     7981 cgctgccgcc gatcccctcg ctcatcatgc tcgccgggat ctactacggc gcccagtacg
     8041 gcggctcgac gaccgcgatc ctcgtcaacc tgccgggcga gaccgcctcc gccgtcacct
     8101 gcatcgacgg ctacccgatg gcgcgccagg ggcgggcggg gcccgcgctg gcgatcgccg
     8161 ctctcgcgtc gttcttcgcg ggctgtgtcg ggacgctgct catcgctttc gtcgggccgc
     8221 cgcttggaga atgggcgctc cagttcgggc cgtgggaata ctgcgcgctg atgctgatgg
     8281 ggctcgtcgc ctcggcggtg ctcgcgcgcg gcgacgcggt gaagggcctc gcgatggtgg
     8341 cgctcggcct gctcttcggc gtggtcggca ccgacgtcaa ttccggcgtg gtgcgctacg
     8401 cgttcgacgt tccggagctc ttcgacggca tcggcttcac ggtgatcgcg gtgggcctgt
     8461 tcgcggtggc cgagatcgcg tcgaacctgg aatcgggcga gcggcgcgag gcgctcgcgg
     8521 cagcgttctc ggagctcctg ccgagccggc atgacctgcg cgactcgagc tggcccatga
     8581 tgcgcggcac cgcggtgggc gcggtgctcg gcgtgctgcc gggcaccggg ccggcgctcg
     8641 cttcgttcgc cgcctacatg ctggagaaga aggtggcgac cgacccggac cggttcggcg
     8701 cgggcgcggt cgagggcgtg gcggcgccgg aagcggcgaa caacgccgcg gcgcagaccg
     8761 gcttcattcc cacgctcacg ctcggcatcc cgggcagcgc ggtgatgacg ctgatggtcg
     8821 gcgcgatgat gatgcacggc ttcgcgcccg ggccgcaggc gatggcggag aagcccgagc
     8881 tcttctgggg aatcgtcgcc agcatgtggc tcggcaacct gatgctgctg gtgctcaacc
     8941 tgccgctgat cgggctctgg gtgtcgctcc tcaaggtgcc ttaccgctgg ctctttccct
     9001 cgatcgtgat gttctgctgc atcggggtct acagcgtgag caacaacccg gtcgacgtgt
     9061 acctgtgcgc ggcgctcggc gcgctcggct ttctgctcgc gcgggtgggg tgcgagccgg
     9121 cgccgctgct ccttggctac gtgctcgggc cgctgttcga agagcacttg cggcgcgcga
     9181 tgctcctcgg gcgcggcgat ccgttgctct tcctcacgcg gccgatcagc ctcgcgttca
     9241 tgctcggaac cgcgctggtg ctcgtcgcga tggtgtggcc ggcgctcagg cgcaagcaat
     9301 aatccacatt tggcggcaaa ccagcgcgaa gagtgcgtac tcgttcattg cggcggacct
     9361 gccggatgcg cagcatcgtc acaatgtctg acatcgccac ccgcctgggg actacgacga
     9421 gcgggcagct cctggttcgg tccggcatcg agatcgagcg ggtcctcgag tcgatcatcc
     9481 gtgaccaggc tccggtgacg gcgaaactga aggacctgat gttcctctcc cgcctggtgc
     9541 actacgaccc ggcagaacag caagttacgg tggcctactg cgaccacaag ccctccaact
     9601 cggcgatgct gtcgctgcgt tccgtgacct tcatgtgcaa ccaccgcggg gcgcagctgg
     9661 cgtttgcctg caccaagccc gttcagagca tgcatctggg caggccggcc atccgcatga
     9721 ccgccccgaa catcctgctt gccatgcagc accgccgcaa ccaggcgcat ggcccgctgc
     9781 cgggcgaggc ggatgtgcag gtcgagctgt cgatgggcgt gatttcgttc gaagccaagc
     9841 tggtcgacat gagcctggac ggcaaggcct ttctcctggg cgacccaggc attcccctgt
     9901 gcgccggaac gcggcttcac aaggcccgca tccgcgacgt ggcgcgcgag cccctgctgg
     9961 tcgacatcga cgtcgataac gtccgccagg ccgtgctgca caacggccag cgtgcgacgc
    10021 gcatcgggtg ccgcatcgtg gccgagcgcg aaaagatgga aaaaatcatt cgcctcttca
    10081 tcatcgacct gcagtagccg tcctttaccg gcagaagaag gcagaattgg gggaagaggc
    10141 tcccccatgc gcatgcgcct gctggtgatg gtcctgttgc tcgccttcgg tacgtccggg
    10201 tacgccgcct atccggaaaa gccgatccgc ttcgtgctgc cctcggccgc gggcggctcg
    10261 gtggacgtgc tgatgcggat cctcgcgcag cagctctcgg cgcagatgaa cgtgcctttc
    10321 gtggtcgaga acaagcccgg cgcctccttc gtgctcggga cgatggacat cgtgaaagcg
    10381 ccgcccgacg gctacacgct cggctacggg aacctggtgt cgctcgcgat caacaagtcc
    10441 ctgctcggct cgattcccta cgacgtcgag aaggacctca ccctcgtctc gaactgcgtg
    10501 cgggtgttca acctgctggc ggtgaacaac aagctcccgg tgagcaccgt tcaggagctc
    10561 gtcgcctacg cgaagaagaa cccgggcaag ctcaccaccg gctcttcggg caacggcacc
    10621 accggccacc tcggcgccga gctcttcaag gcgatgaccg ggaccctcat cgtgcacgtg
    10681 ccgtacaaag gcagcccgca ggcgatcaac gacctgatgg cgggccagat cgacatgatg
    10741 ttcgacaaca tctcctcgat cgggccgcac gccaaggcgg ggcgcgtgaa ggggatcgcc
    10801 gtgtcggggc cgcggcgctc gccggtattc ccggacctgc cgacggtcgc ggaggcgggc
    10861 gtgccgggct acgagacggt ggcgtggggc ggcgtgatcg ggccggccag gctgccgtca
    10921 gaagtagttg tgaggttagt gagagaaatc aaaacggccc ttgccgcccc gaatgtcgtg
    10981 gagcgctaca agcagctcga caccgaaacc gacggcggca cgccggaaga attccgggcg
    11041 ctggtgaggc gcgagacgcc gaagtgggcg gaggtgatcc ggcgcgcggg ggcgaaagtt
    11101 gactgatcag ttcgacctgg tgattcgcaa tgcgaccgtg atcgacggca ctcgcgcgcc
    11161 gcgtttccag gcggacatag ccgtcaaggc aggaaagatc gcccgcatcg ggaaagtgaa
    11221 gcagaaaggc aggaaggaaa tcgacgcggc gggaaagatc gcggcgccgg gcttcatcga
    11281 cgcgcatacg cacgacgacc gcctgatgct ctcgtcgccc gatatggcgc cgaaggtgag
    11341 ccagggcgtg acgacggtgg tggcggggaa ctgcggcatc tcgctcgcgc cggcgccgaa
    11401 cgggatgcgg ccgccggtgc ccgtgccgat ggacctgctc gacgacgagg gcggctggtt
    11461 tcgcttccgg accttcaagg agtacgtgca ggcgctgaag aagaaacccg cggccacgaa
    11521 ctgcgctctg ctcgtcggcc attccctgct cagggtccag accatggacg acacgagccg
    11581 cacggccgcg cgcgcggaag tcaagaagat gcaggacatg gtggacgagg ccatgaaggc
    11641 gggcgcgatc ggcgtctcga ccgggctcta ctacgagccg gcgcgcgcgg cgctgccgga
    11701 agaggtgatc gagatctgcc ggccgctcgc caagcacggc ggcatctact gcacgcacat
    11761 gcgcgacgag ggcgaccgcg tggtcgactc gctcgaggag agcttccgca ccgggcgcga
    11821 gctcggcgtg ccggtcgtga tctcgcacca caaggtcgcc ggcaaggcga accacggccg
    11881 ctccgcggag accctgccga tcatcgagcg cgcgatgcgc tcgcagaaga tcggcctcga
    11941 ctgctacccg taccacgcct cgtcgacgat cctctcggcg agccgcgcgg cgaacgccac
    12001 caaggtgctc gtcacctggt cgaagccgca ccccgaggcg gccggcatgg agctcggcga
    12061 gatcaagaaa acgctcggtc tttcggtgta cgagctgctg cccgcgggcg cgatctactt
    12121 ctcgatggac gagaacgacg tgcagcgcat cctcgccttc gagcagacca tgatcggctc
    12181 cgacgggctg ccgcacgacg ctgcgccgca tccgcggctg tgggggacgt tcccgagggt
    12241 cttagggcac tactcgagag acttgaatct cttcccgctc gaaaccgccg tgcacaagat
    12301 gacggggctg accgcgaaaa ccttcggcct cgccgaccgc ggcgtgctga agcccgatca
    12361 tgccgccgac atcacgatct tcgacccgaa ggtggacgag accgcaacgt ttgcgaagcc
    12421 gatccaggag gcgaagggga tcgagaccgt gatcgtcaac ggcaagatcg tgtggcgcga
    12481 aggcaagccc accggcgcgc ggccgggaaa ggtccttcag cgcagcgcgc ggtagatctc
    12541 cgcgagcgag tgccggcggc cttcctcgac ggcgaacgcg ggcgagccga gctcttcggc
    12601 cagcgcgcgg gcttccggcg cggtcaggtt gcgtacgtgg atgcgcgcga agcagcgccc
    12661 cggacgaacc agcgcctcgt ccaggtcgcc gacgttcggc aggttggtcg agaagatgat
    12721 cttgcggccc tgcgagcgca ccacgccgtc cgcgatggtg aggaagcggt gcaggtgctc
    12781 gttgccgtcc gcgcgcggct tcagcacgtg gtcggcgtcc tcgacgatga acgcgtcgtg
    12841 ccacccggtg atgaacttga cgaacacctc gtcggaggtg agcgccttca tgtcgccggt
    12901 gtagagcgcc tgcgcctgct cgcccttgcg gcgcgacatc tcgcccagga tcgcgcggat
    12961 gaggcgcgtc ttgccggtgc cgggcgggcc ttgcagcacg agcacggtct ccggcgcgtc
    13021 gaggtagcgg gcgacgaagg cgtgcacgcc gccctggatc gccgggtagg cgcggtcgcg
    13081 cagcacgtcg tccgccattt cctcgatcgt cgcgctctcg agctcgccgc gcgcggtcag
    13141 gaagtgccag tcgatcgaga acatcggctc ggtgatccgg gtcgagcctg ccttcgccag
    13201 gatgcgctcc ttcgcctcct cggcgcgcgc caggttccgg gcccagatgt agaacgagca
    13261 ggagcaatag tccgccttgc ggttgccgta ggcggccacg aagagcccgt ccgcgtcgag
    13321 cagcatcgac tcggcatgca ggcgctccgc gcgccacgcg gcgtcgaggg cgatggcgtc
    13381 gagcagtacc tcgagctcga cgcgggcggc gaagcgcagc acccggtaga tcgggtagcg
    13441 cgccacgccc tcttcgagcg cgcgtgcgat gcgcgactcg tggaccagcg cgccgaggta
    13501 atgcacctgc ttcgtcagtc cgttgaaatg cgcgttcatg gttacctgct catccttcag
    13561 ttcataaaaa caaaaaaccc ggggaccttg cggcgcaccc gggcttggga agacggcgag
    13621 cgattgcgat cgctagcacg cctcctgggc ctgggccgca cgcgcacccg cctcgctcat
    13681 cgcgatgggc ttcggttgct gcgtgatgca tagatgcttt ctgaaatctc gatccatatc
    13741 ggttcctcct aacgccgtac ttgccgcggc gttgacgctg cagaaacaaa aaacccggag
    13801 cgcttgcgcg gtctccgtca agtgggcgcg cattctgccc gcgccaatgc caaagtcaac
    13861 acctttttct cagcgctcgt ggccgaccgc ctcgttgagc gtcgtgccgc tgatgcgctc
    13921 cacgcggccg tcgttggtga gctcgaccac ctcgccggcg aggaagcggc tgttgtcgat
    13981 gtcgacggtc tggaagccgc cggcgtccat gcgcaggtag agccggtaga ggttgcgatc
    14041 gggatacttg cccttggcat cggcgccggt gccgggctcg cgcgcgccgg gcaggacgcc
    14101 caccgactgg atcacaccgg agcctgcctt gaagttgccc ggccgcacga tgttggcctc
    14161 gagctggctt tcgtccaagg tcatcgtgca gccgaccacg aggcacaaga ggagcggcgc
    14221 ccggattcgc ataggggagg aggatagcgc ccgggcgccg ctcgcgctcc tgttatcggg
    14281 cagcttgccc cgcgaatgtg gcgtcgttct ggcgcctcga tggaaatctg tcgttgggtg
    14341 tgatcgcatc ttcggtcggc agaagttggc gttgcgggac agaggcttta tcgcgtcgtc
    14401 cgccggctgc acgacacggc gttgcgttca tggtgctgcc cgacgcgccg atgtatcttc
    14461 gtggacatca gccggggcgg cgtcctcggc cagattcttg ctcgctgggt ggaaggaggc
    14521 agccgatgaa gagaagatca gtgcgcagca agacgaaagc aaggaaggtc aagccggtcc
    14581 cggcggggta ccacacggtg acgccttatc tcgcctgcgg cgacgcggcg cgcgcgatcg
    14641 atttctacaa gaaagccttc ggcgcggccg aggtgatgcg catgcccgga cccgacggca
    14701 agatcgcgca cgccgaggtg aagatcggcg gctcgcgcgt catgctgacc gacgaatacc
    14761 gcgagatgga tttcctcagc ccgctcacgc gcggcggcac gacggtgcac atgcatctct
    14821 acgtcgcgga tgtcgacaag atggccgacc gggcagtcgg cgccggcgcc aagctcctgc
    14881 acccggtgca ggacaagttc tacggcgacc gcaccggcac ggtccgggat ccgttcggcc
    14941 acgtctggca catcgccacc cacaccgagg atctgtcgat ggcggagctg cgcaagcgcg
    15001 gcgagaaggc gctgaaggag atcggctaga cgccgaagcg gttgctcacc atgttgccga
    15061 tcgtgaggca ggccgccgag gtcgccagca cgacgacgag cgccatcatg ctgaactcgc
    15121 ccggcaccgc ggcgactgcg ccgaacatcg gggcgaccag cttgccgccg gcgacgccgc
    15181 cggccgcgcc gatgatgatc gagaccagcg tgccgcggtc ttcgttgagt cccagtttcg
    15241 tgatcgccag ccagccgata gcggcgccgg ctgctgccca aaggacgagc tccatttcat
    15301 acctccgttg aaaagcggtt ggaatcgatg agaaccgcag cggcgcgcgc cacgctcgcg
    15361 gctgccgcgc gcgccccggg ggtctgcgcg ctgacctgcg ctccctccat gagaagaaac
    15421 agctgatcgg cgagcagccg agaatcgccg acgccgatgc gcgcgcacag ctcggcgagc
    15481 cgggcgcgca gcctcgcctt gtgcaccttg atcactttcg tcgccgggtg gtcgctctcg
    15541 gtgatctcca ccgcggcgtt cgccatcggg cagccgcgcg ccttcgggtc ggcgacgtgc
    15601 ttcgcgagca gctcgaacgc cgccttgagc tgccggcgcg ggtcatccgg gtactcgcgc
    15661 gacatcgcgt cccaggactg ccagaacctc gcatcgtggt cgcgcagcca cgcggcgacc
    15721 agctcgtcct tggaagggaa gttgcggtac aggctcatct tggtggtgtt cgcctcggcc
    15781 gcgatggttt ccacgccgac cgcgcggatg ccatggcggt gaaacagcgc cgcggcagtc
    15841 ctgaacacgc gctcgcgcgc gctgccctcg ttcttggccc ttttgctcat cgcccgctcc
    15901 cgttgacgct gaggttaccg atcggtatca taccatgtta ccagtcggta tcatcccccc
    15961 accaccggag agcgcgatgc aatggagcgg ccattagcgc gaccctgcgc ggccacgtcg
    16021 gcgaagttgc gatgatggcg gcgatcgtca ccaccatgct gttcctgccc gccgcggtat
    16081 tgctggccgg attgcttttc ctggtcggcg cttcgctgca ggcgttcgcg accttcaacg
    16141 gcgtgctcaa cgcctacctg ggcgtggccg cgtggtggat catcgccttc ctgccgacgc
    16201 ttgcctattc ggtctacgtg atgccgtggg aacgcagaga gccgtgaaga gctccgacgt
    16261 gccgcggatg gcgctgcgcc gcgcggtcga gtggtgcggc gggatcgacg cggtcggcga
    16321 cgcgctcaac gtcgacggcg agagactgaa gaaatggatc gccggcgagc aggaagtgcc
    16381 gcaggcggta ttcgtgcagg cggtcggcct gatcctcgag gccgccgcta cccagtctcc
    16441 gcgcgggcgc acgcccgatt cgaacgacgg cgggcgcaaa gcacgccggc attgatggtt
    16501 gaacgaacaa ggaactcagc atggctgccg gaaaagtgag atggttcagc gatgaaaagg
    16561 gcttcggctt catcaccccg gatgacggcg gcgcggacct gttcgcgcat cacaccgccc
    16621 tgagcggcga tgcctgccaa tcgctaaagc agcaccagcg cgtcgagttc gagttgcgcc
    16681 cggggacaag aggcccgagg gccgcgaaca tcagggcgct gtagcgccgc cgagggcccg
    16741 ctgcgcccac tttgcccagc cgagaagcgc agcgtcgccg tcgaacgccc caacgagctg
    16801 catacctagc ggcaggccgg ccgggccgcg gccgtgcggc aaggtcacgc agggcacccc
    16861 gagcagcgtc caggcgcggt tgaacaccgg gtcgcccgtg ctcgcgagcg aggcgggcgc
    16921 ctcgctcgac gcgctcggcg tcagcaggaa atcgaagtcg cgcatggcct ccgcgagcgc
    16981 gcggcggcag cgctcggcca gcgcgcgcat cgcgtcgtag tctgcgcgcg tcaccttcca
    17041 gccttcgtcg aggcgcggct tgagcgacgc gctgatcttg tcgggatgcg cgccgtactc
    17101 ccaggcgagg gcgcgcgcgg tttcgtagcc catgatcacc ttgtggcggt cgaagagctg
    17161 ccggctttcg aggctcatct ggtaatcgct gaccttcgcg cccgctagtg cgaggcgctg
    17221 cgccgcggcc tcgaggttcg cctgcgtcgc cgcatcggcg agctcccagc gcggcgtgcg
    17281 gcacaggccg atgcggggtt tttcgaatga aggaaagtcc ggaacggcgc ggccgcagag
    17341 cgcttccaat gcgaaagcaa ggtcctccgg ttcgcgcgcg aacacgccga tggtgtcgag
    17401 cgactcggcg accatcttga ggcccgcgcg gttgatcgcg ccgaagctgg gcttgatcgc
    17461 ggcgacgccg cagtaggccg cgggacggat caccgagccg ccggtctggg tgccgatcgc
    17521 ggccgggacc atgaagtccg ccaccgccgc ggccgagccg ctcgaggagc cgccgggcgt
    17581 gtgagcgggg ttgtgcggat tgcgcgtctg cgcgggatgg ttgttcgcga actcggtcgt
    17641 cacggtcttg ccgaggatca ccgcgcccgc gcgcttcagc gcgacgacgc aggccgcgtc
    17701 ggccttcggg cgatggccgc ggtagatcgg cgagttgtac tcggtcggca gctccgcggt
    17761 gtcgatcgcg tccttgatgc cgaacgggat gccgtgcagc gggccccgcc gcgggccgcg
    17821 atcgagagcg cgcgcttgcg ccagcgcgta gtcgcgatcg acgaacgccc aggcgcgcag
    17881 ccgctcgtcg cgctcggcga tgcgctcgag gcaggctgag acgagcgctt cgctggtcag
    17941 ctcgccgcgc tcgatgcggc gcgccgcctc agtggccgaa agcgagaaac tcccgccgtg
    18001 tcttgggtcc ataggtcagg aagtgcggcg acgggttttc cagataggcg gtcttttcac
    18061 gcgccaccgc gtcgttcgcg gtgtagcgct tcagctccga ggccgaaatg ccgtagacct
    18121 tcgcgccgtt ctggcccaag accttcgcgc gcaccgcggg ggtaatttcc gtgtacccga
    18181 accggtctct gaatgcagga gatatctgaa aggtcctgaa cgccacgatc tggtcctgcg
    18241 gcgagccgaa ccagatcgag tcggtgcccc acagcacgtt gtcctcgccg caatgcttga
    18301 tcagcttgcc catggcgtgc gcgccctgct ccggatcgcg catcaggaag cgccaggtcg
    18361 agccgagctc ggcgtagacg ttcgagccgg gagaaacgcc gttcttctgc aacgaggaaa
    18421 taagggtgtc gatgccgtcc cggccgggag acgcatcgta gggcccttcc ttcacgctgc
    18481 tgacgaagcc cgagtggtag atcaggaact tcacgtcggg aaagcgcttg gcgacgacgc
    18541 cgatgtcgct gcactggctg tgctcgtacg agcggctgcc gaagggcaag cccttgtgca
    18601 cgcagatcac cttgacgcca agcttgcgcg cgcgctcgat gaagcgagtg ccgacgtcgt
    18661 cggacaggaa gtagcctttc ccgccgggcc cgtactgcgt gtaggtcttc cacgcgctca
    18721 cgccccattt ctgcgccagc tcgtccatgc cttcgaggtc gccgggctgg ttcgggttga
    18781 cccggccgtg gagcagcagg cggtgcgtgc cctgcagctt gtcgacgatg cgccgcacct
    18841 cgtcggcggc ctggatcgtc accggctcgg aatcgcgggt ggaggggacg aacgacagca
    18901 ccatcaggtc ggtgtcgctg tccatgaaga catccttgat gaagacctcg tggttcttcg
    18961 gatttccctt gggtgtgtcg atgaagtggc cctggacgtc gaagatgagc tcgtccttcg
    19021 ccggaccggt ctggacgcgg gcgagctgcg cgtcgagggc ggattcccgg tcgagatcgt
    19081 agaagccgcc gctcgtaccg tgcgcctggt tgaaggcgag gagggtactc gcggcgccgc
    19141 aggcagagac caggaaagtt cttctcgaag tattcagctt tctcgagttc gctgaagcgg
    19201 cttcgtgcgc aagccggttg gcggcgcggt tcgaggcgga gagcgggaca gggacgaatt
    19261 cgccgttcga cgtggtgtcg agcttgatgg gaaggcgttt gccttcggga tcttcaggcg
    19321 tagtcatggc agcgctctat ggaggtggcg gtcgagggct aggagcaggg cgttcacgcc
    19381 ggcggcgaag gccaccagcc gcagtgtaag ggcaaggata cgctcaatat tgtgcaggct
    19441 catcgcctgc atcagcaggt cgccgagccc gcgctgcgag ccgaacatct cgccgagcag
    19501 ggtgccgatc agggcaagcg agaagcccga cgcggatggc ggtgacgatc tcgggcaagg
    19561 tcgccggaat gaggattttc aatgcaaagc cggtagtcga aagcgcggca ggtgcaattt
    19621 ggcacttccc gcgtgtgaac tgccgcaaca ttgctgcgcc gctcatccaa tcggtctctc
    19681 cgctcgtccg ggatggtcgt aataccccgt caggaacggt attcgttcct cgtccctctt
    19741 gacacgcagg agcaacttag ggccgcaggt tgccaaaaca aacgacgggg ggacccagat
    19801 gaacccgctg ctgcaggtag aagaagtcca ggacatggtg aacatgctgt tccagcgtta
    19861 tccgacgctg tgcggcttct cggtgaagga cgacatgtcc ttctcgaacg tcgcctgcca
    19921 cccggcgctg gccagcgaag aggccgagat gctctgcgag gagatctcgg cgacgctgca
    19981 cgagctgatg gaagaagcgc cggaagccgc cgagatgctg cgcggccgca ccgtggcgag
    20041 gaccttccat tgaaaaagct gctgctcgca gctttccttt tcgccgccgc ctgcgcggcg
    20101 agggcgcagg atctcgatcg gcccctcctc ctagtggccg cgccggatct ccaggggctc
    20161 tatagccgca ccgcgcttct cgtcgtgccg gcggccggcg gccagcacat cggcttcatc
    20221 atcaaccgcg cgaccgacgt caagctggcg acgctgttcc cggagcacgc gccctcggcc
    20281 aaggtcgccg acccggtcta cttcggcggc cccgagatga tcggctcgat cttcgccatc
    20341 gtgcggcgcg atcccggcgc tccggcgatg cacctgttcg gcgatctctt cgtcaccggc
    20401 aacggcgccg cggtcgatcg catcatcgag aagaccccga acgacgcgcg ctatttcgcc
    20461 ggtttcgtcg gctggggaat gcacgagctc gccaaggagc tcaaggacgg gttctggtac
    20521 gtcgcggagc ccgacgcggg gctgctcttc agcaaggacc ccgccggcat ttgggaagag
    20581 ctggtgaagc gcgccggcgg cagcccgctg cccgcgggcg cgaactcaat ctagtagcgc
    20641 cgtcccctca gtacgctctc ccgtcagtat cgttgtcctt ttattgtcca ggggatgcgc
    20701 ggcccgcctt ccccttcttc ctgcggccgc acggcgccct tcttcgggcg gcccggccgg
    20761 taggtgacct gaaagatctt caggggcggc actttcgggg ggctctcgag gaaccggcaa
    20821 tccgattcgg tcggcacgct gagcagcgcg ccgaggcgcc ggccgtccag cagcaccgtg
    20881 tagcgcagca tgtgggccga gccgctggcg ggctgggcgc ggatctcgta cttgccgatc
    20941 ttgaaggtcg tcaactcggg tttctccacg gaagcgaact cgtatagccc acatcggcgc
    21001 cgagcgccac ctctatttcg tggcgctcgc gcgacgggca cgccgcttgc tccctttgcc
    21061 ggggcaatcc cagaaagggg cacctatgga acacacgcag atcgacaagg taccgagcaa
    21121 cgtgcaggaa gtaccgggcc gcggagaaag cttccaggcg gcgcgcgagc ggctgaacca
    21181 gacgctttcc ggcgtcagcg agcggggcct taatgcggca cgcgaagcgg ctcactatgc
    21241 cgaccgcacc gtgcacaaca gtccgtggac ctcggtcggc atcggcttcg gcctcggcgt
    21301 ggtcgtcggc gcgctgatcg ccatcgcggc cggctcgaga cattcaaggt tcggatagcg
    21361 agcggctaga atcgcgttcc ttccaaccct ggggaacgcg atgaaaatct cgatgtacca
    21421 ggcgagcgcg cctcgctttg cgaacgcgct gcgcaacctt tcggccatcc tcgacaaggc
    21481 gcaggcgcac gccgaggcga aaaagctcga cccggcggtg ctggggagcc tcaggctgat
    21541 cgccgacatg ttcccgctgg cgcgccaggt gcaagtcgcc tgcgacaccg ccaagggcgc
    21601 gctcgcgcgg ctcgccggcg ccgagattcc caagtacgag gataccgagc agaccatcgc
    21661 cgagctgaag gcgcgcatcg cccggacgct cgacttcgtc gcctcgctgc cggcggcgaa
    21721 gatcgacggc tccgaggagc gcgaagtggt gatgaagatg cgcggccagg acgtgaagtt
    21781 cagcggcctg caatacctct tcggccacgc ctggccgaac ttctacttcc acgtcacgac
    21841 cgcctacaac atcctgcgcg cgaacggcgt cgagctcggc aagcgcgact tcatcggcaa
    21901 tccctgactt cgtagcctca ctgcttgaac ggcgcgccga gctgctcgca ctggctgcgc
    21961 gcgtactgcg cgccgacgcc gaaatcgtcc gggccgctgt tcacctgcac gatcttctcg
    22021 tggcggatga agaccgtcat cgggtagaag ccgctgaagg cgcccgagcg gttcttgtcg
    22081 ttcacccaca cgcacaccgc ccagccgaag tgcatggggc ggaacgcgac cgcccgctgg
    22141 tacatctgcg ccggctcgtt gcggaactcg atgatcgcgg ccttgggatc ggtgagccgc
    22201 accgcgaggt agctggtgat ctcctccttc cagcgcaccg gcctcgggcc gtagtcgagc
    22261 ccctcggttt ccgctttcgt caccggcgcc ggggcgcagg cggcgaggag ggcggcaatg
    22321 gggatagtca gcaggaccgc gaggcgcata agctgctccg tctggtaaaa ggcgaattat
    22381 gcaacggtgg gtggagcgcc ccgaagcccg ctgggcgttc tgggcgggca tggtcggcgc
    22441 cgtagcgatg gggctgctgt cgacctacag gatcttatct cagggttccg cggcagcggg
    22501 gttcgcgctg gtcttcgtgc cgctcgccgc cgccgcggtg gcggtgccag ccgccgtgtg
    22561 gggcgcggcc ctcggccacg tggtgctgca cctgcgcggc cgggcgggcg agcctcggat
    22621 cgtgttctgg gccgcgctca tggccgccat cctgccgccg gcggcactgg cgtgggagct
    22681 ctggcgtcgc tgaggcgccg cgcgccctgg tagaggagcg ccatcgccgc gatctggatc
    22741 acgtggtaga ggtcgttgtg attgaagtgc gggtgcagcg agaacccgct cgcctggatc
    22801 agcgcgccca cgaccgagag cgccacgccg ccgagcagaa atccattcca cttccagaga
    22861 tgcagggcgg cgacgaccgc gaaagcggcg ccgctgtcga cgaccacgaa aatgaaatcg
    22921 tcccgcacca tcatccagcc gccgtagatc agcagcttga tcaacgccag gcccaggacg
    22981 agcttgcgcg ccgcgcccga cagggtggcg atcgccgagc cggcgaccat ggcgaagctc
    23041 gccgcgccca cggcgagcac ggtcgccttc cacaaaaacg cgtgctggaa gaaaccgtgc
    23101 caagtgccgc cgaggaacgc gccaagcgcg agcgccgcga acgccgcagc ccaggatttc
    23161 ctggaatcct gggattcgga attccggagc aacaacacgg cgagccaccc cgtcacgcct
    23221 gccaggaggt agtcggtcac cagcgtggtc ggctcggaga tgctcaccgg cgcagccgct
    23281 ggcggtggga atgaccgacg gtgatgacga aggcggccag cacccagtgg aacacgatcg
    23341 ggatcgggtg atcccagagg agcaccgcgc gcgcgaccat cgccgcgaga tagacgtagc
    23401 cgaaccacag ccacgccgtc gcgaaggatt ttttcggctc gaagaagaag ccgcgccggc
    23461 gggtgaagtc gaggcagatc ttcgtcatca gggcaatgat gaggatctgc gatggaagca
    23521 ggtattcgta gggcatgagg ccggaatacc aagcgtccat cggcggcagg aacccgacgt
    23581 tgagaaagac caccagcgcc tgcccgagga ctcgcaggac gaagagcgca agcagcaccc
    23641 acaagacaaa gctatttctt ctcgtacatc ctttcgtagt gccggtacca ctcgctctgg
    23701 aagagcttct gcgggtcgta tttcagcttg agcttgagga attccggcag ctgcgggtag
    23761 cacgcgtcga cctgccggcg cagggcgtaa cggttgtagg tcgggtagta gctgccgccg
    23821 tacttgaggc caatgtcgat caggcggcgg aacacgtcgc ccgccttgat caggccgctc
    23881 gtggtgtgct cgacgtgcac gttgaagatg acgcaggcgt agggcctgcg cgcccaggcg
    23941 aggaagctct cgcggtcctg ctcgatcagc cgcaccgtgc cgtagatgat gtcgaccttg
    24001 tcgcgccgcg cgtactcgcg cacctccatc atgaagcgct cgagcgcgtc gcgctcgcag
    24061 tagatctcgg tgatcgcctc ggtcgcccgg acctgcgcgc cgacgcgctg gtcgagcgcg
    24121 cggtggtagt tctcggggta gacgctcatc tgcgcctcgt cggaccagta aacgtggccg
    24181 ttgctcgcga cgtagtggcc ggcgtagcgc cggaacgcct cgcccttggc gtggtgggcg
    24241 agggtcagga gctcgatcca gtcgcgctcc agcagctctt tctgccctgg cgggatcggc
    24301 gtcgccgggt cgaccggccg gtagcacgag aacacgccgc gattgaggaa atcacccgag
    24361 gtctcgtcga tcgcgtactg gaagtcgccg tagaggaagc cgtcgtggat gcgctcgccg
    24421 aacgccttcg gcaggccgtc gatcgagcgc acctcgacca cccgctccag cttgcgccgc
    24481 tcgaccaggc gcagcgtcac cgaggtgatc aggccgaaca ggccgtagcc gccgatcgcc
    24541 acgcggaaca gctcggcgtt gtcggtgcgg ctgcagttca ctacttcgcc tttgggcgtg
    24601 aggagcctga aggactcgac gtcgccgatg aacggcggca tcttgaggcc gcggccgtgc
    24661 acgttggccg cgaggcagcc gccgatcgtc agcttgtcgg cgccggtctg cttctgcgcg
    24721 aaggtccact ggcgctcgcg ccccttctgc acggtcacca ggttctgcag gacctgcggc
    24781 cactggatcc cggcctcgac ctcgagatgg cccttctccg ggtcgaagcc gagcacgcgg
    24841 ttcatgcgcc gcgtgtcgat catcaggccg tcggcgagga actgctgccc gcccatggcg
    24901 tggcgcgcgc ccgagatgca gaccgcgcgc tcttccttct gcgccgcccg cagcgcggcg
    24961 cggatgccgt cgagcgtgtc gggctgcacg atgcgccaga cgcgcgacga gttgagctgc
    25021 gaatggacgt cgttgaccag aatgcctggc gggggttttt cctttttttg tggaaaaacc
    25081 gtgcggggcc aggcggcgcc ggcaagcgtg atcccgagga aatctctacg ccggaacagg
    25141 cctacagctc ctcattgcgc aacggagtag accccttctt cggccggccg ggacgattgg
    25201 tcagcgcaaa gatgcgcagc ggcggcaccg gcggcggggc ttccatgaag cggcaatcgg
    25261 actcgctcgg cacgctcatc agcgcgccca ggcgcctgcc gttcagcatc accgtatacc
    25321 gcagcatgtg cgccgagccg agctgcggct gcgccacgat gtcgtacttt cccagcttgt
    25381 actgcttgcg ttccagcgcc ggcaatgcca tcgggatagg gagcgctatt tcggggccgt
    25441 catttcttcg ttgccgagaa cacgctcgac aggtcgactt cgtcgagctt caccgcggcc
    25501 ttgttcgccg cttcgatcga cgccctcatg cggtcggttt cccgggttgc ctgctcgaag
    25561 gcgtcggcga gcttgaagct gaacgcggcg agcgccttgg ggtcgggctt cttctggtcc
    25621 gatccggcga gggtgccgta gacccggctc gacagctgca cgaagatccg ctcggcgagc
    25681 tcgacgtacg ctttctgggg atcgtgcgac gccgaggtct ttgccggcgc gtccttgctg
    25741 ggtgcgggtg cgctcatgat cgttccggtt tgattaccgc aatcctacgc tgatcttcgc
    25801 tcagggtgcg ggcgagttgg tcccgcagct cgttggaaac cgtgacgtgg gcgccgggct
    25861 ggtcgcggtg cacctcgtag agcttggcca cgacctcgat gtcgtgctcg gcgaaggtct
    25921 cggcggtgcg gcgcgcgcgc tcgggatctt tgctcgcgtg cgccaccgcg agctcgccca
    25981 gcttgagcgc cgagagccag gtctcgcgct cgatcgcctc gacgccgacg tcgcgcagct
    26041 tgaacatgtg gcgcatgtcg tgcgcgcgcg cgatgacgcg cagccgggga aaatgagtgc
    26101 gcgccacttc ggcgagccgc gtgatcgcct cggcgttgtc gagggcgatg atcaggaagt
    26161 cggcgctgtc cgcccccgcc gcgtgcagca gatcgaggcg cgtggcgtcg ccgtagaaca
    26221 cgcggaagcc gaacttgcgc gactgctcga cgtggtcggg atcgtcatcg agcacggtgg
    26281 gctggaagcc ggcggcgttc agcatccgcg ccacgacctg gccgacgcgc ccgaggccgg
    26341 cgacgatcac gttgctcgcc tgcgggacat cgggcgcgcg ctccggcgcg ggctcggcga
    26401 aataaggcgc gagctggtcg tggagcgcga gcaggaaggg cgtgagcagc atcgagatcg
    26461 ccaccgccag ggtgatcgcc tgcgcgatct cgccgccgat cccgccatgc gtcacggcga
    26521 ggccgagcat gacgaacgcg aactcgccgc cctgcgcgag cagcacgatg aagagcgcgc
    26581 gctgcggccg cgggatgccg gcccagcgcg cgatcagcca cagcaccgcg cccttgatca
    26641 ggaacagtcc cgcgatcagg ccgaacacca gccagggccg ggcgccgagg acgccgaagt
    26701 cggcgctcat gcccacggcg atgaagaaaa gacccagcag gagccccttg aacggctcca
    26761 ccgcggcctc gatctcgtgg cggtactccg actcggcgag cagcaccccg ccgaggaaag
    26821 cgccgagcgc catcgacatg cccgcgtgct cgaagccgag cgcgatcacc agcacgagga
    26881 gcagggcaaa cgcggtgaag atctcgcgca ggcgcgtgcg cgcgatgtgg cggaacaccg
    26941 ggcgcgcgac caggcgcccg ccgcccagcg tcaccacgat caccgccgcg gcgagcccga
    27001 gcttctccca cgagaacccc gcgccgccgg cgccggtgcc gaggagcggc agcagcgcga
    27061 gcatcgggat cactgcgatg tcctggaaca gcagcaccgc gaacgtgccc tggccgccct
    27121 gcgtggtgag cgctccgcgc tcggtgagcg gcgcgagcgc gagcgcggtc gacgagagcg
    27181 acagcgccat ggccgcgatc agggcggcgc gcaggtcgaa gccgagcgcc cagccggcga
    27241 gcgcgagcgc ggcgatcgag ccgagcacct gcgacaggcc caggccgagg agcttggcgc
    27301 gcatgttcca cagccgcttg ggctcgagct cgaggccgat cacgaacagc aggaacacca
    27361 cgccgagctc ggcgaacgcc ttgatatggt cgaccgtgtc gaccagctgg aagccccagg
    27421 ggccgatggc gatgccggcg agcaggtagc ccgccaccga gccgagcccg agtttgtgag
    27481 aaatcatcac ggcgaccacc gccgcggcga aatacaggct cgcctggacg agaaaatcgt
    27541 tgccatgctc cacggccgaa gtatatttgc agggggaggg atcaagaaaa tggactccgt
    27601 ggaagcggag ctgcaggggc gcgtcgcgcg cctgttcgcg cgccgccccg agctgtggcg
    27661 cttcagcgtg cgcgaccgca gcatgctgcc ggacaccctc gacccgacct cgctcgcggg
    27721 cgagctcttc atcttcgaga tcggcctgtc gccgcggcag ggcaaggagc attacgacga
    27781 ggtctacgac gacatcgcgc gcgccatcac cgccgcggtg gcggcgcggc ccgaggcgcg
    27841 gcgtttgctc cccgggcgta ctttcgtaag agccctgcat tagactttcg gggtgcgtgc
    27901 cggcatcccc tataccctcg ccaccggcga gaagctggtc aacgagacct tcgggccgaa
    27961 caacatccgc cggcgcacca gcggcgccca ggagctgcag cccatggaaa tcgcgaacgg
    28021 ccgcgggaaa gccacgtccc tcgatgaaaa cggtttcgtc ttcgtcgagc acaagaccgc
    28081 tgtcaaagat ttcttcgacc cgaacgagct cgagaaggtc tactacgccg aggtcgtggc
    28141 gttggtcacc aggatctcgg gagcaaatcg cgttcaggtg ttcgaccaca cgcttcgctc
    28201 aggcgacgag tccgagcgcg aggcgaggct cgtgcgcgag ccggtccttt ccgcgcacaa
    28261 cgattacacg gagtggtcag gccccaaccg ggttcgcgag ctgcttcccg acgaagcaga
    28321 ttccctgctg aagaaacgct tcgcgatcat tcaggtctgg cgcgcgatta atcggccgat
    28381 ccagtcgaac ccgctggcga tcgccgacgc aaagagcatc gcccccgacg acctgctgat
    28441 cgccgagcgg cgctacccga accgcgtcgg gcagacctac cgcctgaagt attcacccgc
    28501 ccaccgctgg ttctacttcc ccgagatgcg ccgcgatgag gcgctggtat tcaaggtgta
    28561 cgactcggag aaggatgggc gcgcacgctt cacgccgcat acctcgttcg acgacccgtc
    28621 gaccccgccc ggcgcgccgc cgcggcagag catcgaggcg cgcgcactcg cttttttcta
    28681 gaggctgggc cgcttcggtt tcctcgcgag gaggaagaac cccgagccga ggccggcgag
    28741 gatcgcgatg atggtgaagc cgacgcgata gtcgccggtc atgtcggcga agatgccggg
    28801 gatgatcggc ccgccgacct ggcccacgac gatgatcatg aaggacaggc cgaggatcat
    28861 gccgatcgcg ctgcggccga agtagtcggc gcgcatcgcc tgcatgaacg ggccgcgcag
    28921 cccccaggcg gtgccgtgca ggacggcgaa tgcgaccacc atcagccaat tggacgcata
    28981 ggtgagcaag agcaacccca gcgcatgcgc gagcatgcag gcggcggcga tcaggcgctt
    29041 cttgtatcga tcgccgattg cccagccgag gaacacgccg acgacctgga agatggtgac
    29101 caggctgatg acgaacgaag ccgccgccac cgagtagccg aggccttcct tgaggtgcgt
    29161 gatcgcgtgc accgagaccg cgtgcacggc gaacagcgcg aagccgtgtc cgagcgagat
    29221 cagccagaag gcttgggtgc gcagcgcctc gcgcgcggtg aaatcgcggg tcgaatctgc
    29281 tgcttcagga gtttggtttt tttcaggtgc ttcctcaggt atcccgtcca cgacttcccc
    29341 gtgctcgtgc gggctgttgc ggattaccag cgcgagcggc aggccgatgg cgatcgcaat
    29401 caggccggag gcgagggcgg tgccgcgcca gccgaaggtg gtcagcgact gcgccaccag
    29461 cggcaccatg atgccgcccg ccgcgagccc gaaagacatc gccgagagcg cgcgggcgcg
    29521 cttgcgctcg aaccagtgga tcagcgcaac gttgagcggg aagtagccgc acaggctcgc
    29581 gccgagggcg atgacgatga acgccccgta gaaggtcggc agcgaagtca cctggcttag
    29641 gaggcacagc ccggcgccga acacgaacac ccctgcgcgg acgaagacct tgggaccgaa
    29701 gcggtcgatc gcccagccga gcaccgggcc gagcagcgcg acttccatct gctggagcgc
    29761 cgccgcgccc gatagcgcgg tcttgctcca gccctgctcc tcggcgagca ccgcgagata
    29821 cgcgccgaag gcctgcgtca cgagcccggc ctgcaggaat tgcagcccga ccgcggcacc
    29881 gaccatgcgc caaccgtaga agatcttctt gtcggccaag cacgacagga taaccctcgg
    29941 gtccgttact tgcggaaaaa gggcatgaaa ccagcccagc tcggcgccct caacggcgat
    30001 ctcgcgccga ttgaggagga cctggcgacc cgcatcgagg cgattttcga ccgttgcccg
    30061 cagcttttcg ggttcacggt gcaggattcc tcggcgctgc ccgaggagct gcgctcgatc
    30121 gcgctcgagc gcgagctggt ggtgagcgat atcggcgtgt acccgtacct gaccgccgac
    30181 cagtgcgagc agatctacaa cgaaatcgcg gtggcgctgc tcgacttcat gttcgagcgc
    30241 ccggcggcga aggacgtgct acgcgggcgt acgttcgtcc gtaccctgca ctagggaaac
    30301 gcaggcttcc tcgaaccgtc ccgctcccat ggtgcccggg gcgaagatct cgcgctcggc
    30361 gatccacttg aaggattcct cgaacacctc gcgcgtgtag ggctcgaaca cgatgcgctc
    30421 gcccggcccc cagcgccgcg tgtcgatctc gccctggaag cgggcgggaa actccttggc
    30481 gtagtggcgg gtgtagagct cgggacgcag gtcgatgtcg cgctgcgcgc ggcgcagcgc
    30541 ccggaaatac ttgcgcacgt cctcgggatc gggatcgccg gtgagcatcg aggccatcat
    30601 gaaggtggtg tcgaggatct tgcggaagcc gagctgctcg aggaagtagt acggcccgct
    30661 gaagagcgcc gccgcgggca cctggcggtc gatcagcttt tccatgcggc cgaagagaat
    30721 cccgtcggcg aacgagagct tgatgtcctc gcgcggcagg tattgctcga gcgcctgcag
    30781 ggtcgaatag tggctgcccg actggtggcc gaccgagatc ggcaccccct tcaggtcctt
    30841 cggctcgcgg atcggcgatt gcgccggcac gaatacgccg cccggggaga ctgaataaca
    30901 gtccgcgacc atctttccca ggccctgcga cgcggcgaca ttgaccgtcc agtggcaggc
    30961 gcagctgacg ttcgtttcgc gcccttcttc gaagctcgtg taggcgccca ccttgttcgg
    31021 cgtagcgtgg ctcttgaata cttcgcgaaa ttcgtagtcc aggcccgcct cgcggaagta
    31081 gcccttttcc tcggcgaccc actcgtgcag ccggaaatgc ggggaaatca cgaacttggc
    31141 catgccctaa tctcctctga cccggatcat gccaactatt tcgcaccgtt gacagatgct
    31201 caaccccctg tagcggcttt cgggacagca tcgcgttgta gggaagaggc ctagaccggc
    31261 cggccccggg gaggggcaac cggagtgcaa cgaaccggga atagcttaga tgccatgaaa
    31321 accgatcgcc acgtccagga gcagttggaa tcgctcttcg agcgttgccc cgacctgtgc
    31381 gggttctccg tgcgggagga ggcccaggag ctctatgtga gcgacatcgg catctcgccg
    31441 cgcatctccg ccgagcagta tggcgagatc taccaggaca tcgcccagac cctcggcgag
    31501 ctgctcgagg aggagcccct ggcgggggac tggctgcgcg gccggacctt cgcgagaact
    31561 ttgcactagg ggttcgtctg tagttgtttc agggattccc cgattggccg tttcgctaca
    31621 aggccttact ctgggatccc atgaagacac tccagcagtt cgacagcctc ctgggaaggc
    31681 tggaagccga cgtcgacgcc cgcatccgct cgcttttcgc ccgctgccct accctgttcg
    31741 gcttcgccgt gcaggaccgc gccatgctgc cgaaggacgt cgatcccaac cgtattcccg
    31801 atgcggatct gttcgtcacc gacatcggca tctatcccaa gatcgattcg cagtacgacg
    31861 agatccacga cgagatcacg atcgcgatct cggacctcgt gcacgaccag ccgcacgcgt
    31921 acgactacct gcgcgggaag accttcgcgc ggtcgctgca ctgatccaga aaacctgacc
    31981 gagaaatccc aagggggagc ctgagcgcgt gcgccacagc ctgggagggc tgtggcgcgc
    32041 agcgccgtgc aggtctgaca aagtgcaggt ctaacaaagt cttgcccacg ccggcgaggc
    32101 ccgcgggtaa cttccaccca tgggctaccg catcgatttc agggtcggcg gcggagttct
    32161 ggaagcctcg ggggagcacg tcctcgtcga cgtccgcggg ctgcgcgaca gccacggcag
    32221 gctgcgcacg ctcttcgccg cgaaagaact gccgccacgc gtcgcggtga tcgacgcatg
    32281 gcgcagcgac cgctattgca tcttcaccga gatggccgcg cgccgcctcg gctgcgacct
    32341 caggcgcttc gaggatcacc ggactgcgct cgcctggctg cgcagcgccg cggcctagcc
    32401 tcgccaatga gctaccgcat cgaatacagc gtctccggag gggtgctcat ggcgaccctc
    32461 accggaacct gtccgacggc gggcttcatc acgcgcgaga tcagccggca agcgcgcgag
    32521 aactcggcga gctgcctgct cgtcgacgtg cgcgggctgc gcgaccgcgc cgggcgcctg
    32581 cgcgaggtgc tcgccagccg ca
//
LOCUS       DATBSZ010000142        23419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1182425, whole genome shotgun sequence.
ACCESSION   DATBSZ010000142 DATBSZ010000000
VERSION     DATBSZ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23419
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182425"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VIG70_09585"
     CDS             <1..766
                     /locus_tag="VIG70_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8250847.1"
                     /translation="ANALAKVRKLINSKKHRVPLERAKLLPPISNPCLLLSVGMNYHE
                     HLKEMKTPAPENPAAFTKSVASIIASGQPIKVPSSNPSMLDWEGEFTVVIGKGGYRIP
                     AAKALDHVAGYTMVNDVSARDWVAAIFSSTGVMGPIHAWEHNLLGKMFPTFCPMGPCI
                     ATRDEIPDPANVRIQTRLNGHVMQDANTDDLVFSVVKLIEYYSQFYRFQPGDVITTGS
                     PSGVGFGRNPKVFMKPGDVVEVEVKEIGVLRNPIEA"
     gene            771..1850
                     /locus_tag="VIG70_09590"
     CDS             771..1850
                     /locus_tag="VIG70_09590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8250848.1"
                     /translation="MEFGYFTLSDNHYPNNPRSANQLVLEIREQALLADKIGMHSAWI
                     GEHHFDSLGVNSRPDLVLANIASVTKNIRLAPAVTVLPLHHPVHVAEMWATLDVLSGG
                     RVDFATGRGYDRKEYAPFGADFMASAEIFEEGIDVLLKAWRSPDKWSHRGRFYNIPEM
                     AITPSPLQKPLPFYVASFSKTSVDIAAKRGLNIIYAPFAAGMVFGGLDKAVETYREAC
                     AKAGNKPGRAMCSYFIYIADDARQEDYGRQTQMDYFNHCVLQAFPQKKEDAPPTMQYF
                     MKIVDILKNMKKEDLSDKSILLGSPQKIVDTLKRVEKSGIDEVILYFNVGNKPHNLVK
                     EQMHRFMQEIASQFERKPVAYGTLS"
     gene            complement(1893..2630)
                     /locus_tag="VIG70_09595"
     CDS             complement(1893..2630)
                     /locus_tag="VIG70_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8250849.1"
                     /translation="MSNIILVTGGSRGIGAATCRLAAEQGYAVCVNYLKNKQAADAVV
                     KSLKGKAIAVAANVADERDVQRLFETVDRDLGRIDVLVNNAGILAPASRLENFDLARI
                     QRIFSTNVFGAMLCAREAVKRMSVRHGGRGGAIVNVSTGFVKSGAPGMFLDYAASKGA
                     IEVFTGGLARELATEGIRVNAVRPGLIDTEIHADGGDPERVKKAEHVAPMKRPGQPEE
                     VARAILWLASDAASYTTGTVLDVSGGI"
     gene            complement(2771..3085)
                     /locus_tag="VIG70_09600"
     CDS             complement(2771..3085)
                     /locus_tag="VIG70_09600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY8250850.1"
                     /translation="MNKFVLIVEFDVKPEHLAQFNQLIAVNAAASVADEPGCRQFDVL
                     QAEDNPSRIVLYEVYDNPAAFQDHMGRKHTQTFLAAMKPLITKQTAYKLKRTVAPPVK
                     KG"
     gene            complement(3082..3429)
                     /locus_tag="VIG70_09605"
     CDS             complement(3082..3429)
                     /locus_tag="VIG70_09605"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8250851.1"
                     /translation="MPSSEAWAGGWGFISGLGPVDLENAAKALPEAVEDQAHRILSNL
                     EALLKKRGLAREHVVSVRIHLVEFKRFHERLDRAYAAFFSGARQPARSCIGVSALARG
                     ALVEMDFIVKEPS"
     gene            complement(3416..4756)
                     /locus_tag="VIG70_09610"
     CDS             complement(3416..4756)
                     /locus_tag="VIG70_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8250852.1"
                     /translation="MRTNAPYSGYYNRPPQDEGHLLTRVGPGTPCGEYLRRYWHPFML
                     ASELKDLPVAVRLLGEDLVTFRDQSGRIGLLHRHCAHRGASLEFGIPAERGIRCCYHG
                     WQFDVDGTILDTPAEPTDRIKSRIVQGAYPLREAYGLLFAYMGPPESLPEFPVYDSFE
                     YPEDNQLVAFNMHLPCNWLQIIDNGADPMHNAFLHAIVSGEQFSPVFKVLPQLDFPET
                     PMGYLSMATRKVGDYVFIRASDMMLPNVGQFPNGMNMAQRETFGVRPYLTRWAVPLDD
                     THSLYIGVAHLNSYNNPQGSLKPEQFGVDMIPFIGQTGDRPYEERQREPGDYDAVTSQ
                     GRIANRAAEHLATTDRGVALTRRMLTSAIGVVEKGGTPVMPRLYPKTARVRTYAHETV
                     VRVPSPGALSDPKALAEFGRRAAMAYVETDDLPPKERELEVARRIRGLFEHAEQ"
     gene            4832..5509
                     /gene="cecR"
                     /locus_tag="VIG70_09615"
     CDS             4832..5509
                     /gene="cecR"
                     /locus_tag="VIG70_09615"
                     /inference="COORDINATES: protein motif:HMM:NF008587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CecR"
                     /protein_id="HEY8250853.1"
                     /translation="MSSVLAADLGRGAQARLALVHAAVQVFGESGLEAATTRDIAQRA
                     QQNIAAIAYYFGGKEGLYRAVVEHIVEVILARVGPLMDEAEAFLEREQQPSERCLDYL
                     KRLLAGSIATNQSMIPVTGIIVREQTQPTRAFSILYDGCLERLQRLGAGLIRAYLGEA
                     VPRADCIVRFHALLGESLAFRFARETILRRTGWKEIGAPEEARIQSIVTEHAELVLRG
                     LRRKTRR"
     gene            5528..6493
                     /locus_tag="VIG70_09620"
     CDS             5528..6493
                     /locus_tag="VIG70_09620"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250854.1"
                     /translation="MNRYRILALVLALCAPAFASAQAFPSKPIRMIVPYAPGGATDVL
                     ARPIAQRLSEQVGQPVVVENRPGANATIGADHVAKSPPDGYTFFLGSIVHYMVPFFSK
                     NVPYDPVKDFTPLALVAVVPNILAVNPSLPIHSVKDLIDYAKKSGVKMHYGTTGTGST
                     HHLGGIFLAQTAGIPLEHVPYKGGNPAIQDVLSGSIPLVILTATTVMPQVKNGKLRAL
                     GLIENRRFDVVPGVPTIGESLPGYAVPDTWFGVLGPAGMPRPIVERLNSELRKAVQAP
                     DVRQRIENIGMVPTGTAGIDEIAANIASEVDTIRKIVTAADIKPE"
     gene            6490..7422
                     /locus_tag="VIG70_09625"
     CDS             6490..7422
                     /locus_tag="VIG70_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8250855.1"
                     /translation="MKLATFQEGGASPAVGAVEGDVIVDLSGHFPSMLALIDGGEAAL
                     ELARKAVKRSAVKKKLSQVRLLSPVPEPRQIRDFNNFEQHIRNAPWGVAKLRARLAGQ
                     PEPDRSKAPAVGEITFKQPIFYLSNRFNVVGHEAEVQWPGYSEWLDFELEFGVFLGRK
                     GKNIAKERAAEHVFGYAVFNDFSARDRQVREMEGWMGPTKGKSFDTGNAIGPWIVTKD
                     EIPDPQALAVSLRVNGEVWAKNTTAGMIHSFEDMISFVSHDETLHPGEFFGSGTIGGC
                     CGLECDRWLKDGDVVEAEVEKIGVLRNRVVRRKR"
     gene            complement(7423..7929)
                     /gene="nuoB"
                     /locus_tag="VIG70_09630"
     CDS             complement(7423..7929)
                     /gene="nuoB"
                     /locus_tag="VIG70_09630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEY8250856.1"
                     /translation="MLRNLVGGPLTEPAPPADEAALAELAARVNQASRARLGRTLSIR
                     EVDAGSCNGCELEIHALNNVLYDLERFGLRFVASPRHADVLLVTGPVTANMREALERT
                     YQATPDPKWVVAVGDCARNGGVFAGSAACVGGVSAVLRVDLHIPGCPPSPVALLKGLL
                     ALVESVST"
     gene            complement(7934..9205)
                     /locus_tag="VIG70_09635"
     CDS             complement(7934..9205)
                     /locus_tag="VIG70_09635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY8250857.1"
                     /translation="MKSKIHLLDLPCEENRFPSVGRVHPPAIRLERAVRDLAGLEPEG
                     APDTRPWLRHAGAYAFLPAEGEGLHQIPVGPVHAGIIEPGHFRFTANGETVVRLEERL
                     GYVHKGIDALMAGAPLERAAALAGRVSGDSTVAYALAFSMAVEAALGAQVPPRALWLR
                     ALAGELERLANHFGDIGAVCNDASFSIMHASCGLLREQVLRASNACFGHRLMMDCVVP
                     GGMRADLAAQGEREVRALLALVARRFPELVDLYENTASLKDRTVDTGILSPALAQQYA
                     AGGYVGRASGRHFDARRAPGYAPYGELEFEVPVVQAGDVNARVWIRMQEVEQSLALLE
                     QILERLPPGPVFSPIAPAQGEGTALVEGFRGDIFAWIRIGADGRVERSHLRDPSWFQW
                     PLLEAAIEGNIVADFPLCNKSFNCSYSGHDL"
     gene            complement(9306..10751)
                     /locus_tag="VIG70_09640"
     CDS             complement(9306..10751)
                     /locus_tag="VIG70_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HEY8250858.1"
                     /translation="MTGGSLVLAAILVIPALGAGVLALLPDDRVSARLNASVSFLTLA
                     CALVLLVERPAPGPFLIVDDLNVVFIVLSTLVGFTTSVFSGSYIAHELETGQLTPAYL
                     RFYHAMYQVLMFGMNLALLSNNIGLMWVAVELATLTTVLMVGIYRTPAALEAAWKYFL
                     LGSVGIALALFGTILVYLAARSVVGEGLDAMAWNVLVEHAAQFDPALLNLSFVFLLLG
                     YGTKVGLVPLHAWLPDAHAEGPTPISAVLSGLLLNVALFAVLRFKSIMSANPAALAPG
                     PLMVTLGLASVVFASFMLYRRQDIKRLFAYSSIEHMGIVTFAFGMGGTLANFAGLLHM
                     TLHSLTKSAIFFCVGHIAQVKGTQRMADIRGLTASHPVLGWSLSLGVVAIAGLPPMGL
                     FMTEFLIVTTTFAREPALAVILGLSLLVALGALLLRLNGLAFGDPDGRRDPAKASFLP
                     VALHLVLVLAAGIWLPPALVSWFQHVATLLN"
     gene            complement(10748..11413)
                     /locus_tag="VIG70_09645"
     CDS             complement(10748..11413)
                     /locus_tag="VIG70_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HEY8250859.1"
                     /translation="MSGQLAFDVAHLFAGGLVLMSFMLLYQDRMTGLINTFALHALVV
                     SLSVAWQAAIQDAPHLYITAAIALVVKAVVIPLALHRIVVRLGIHRTIEPVVGVGLTM
                     LAGIGLVALSIMVMLPITEQAGTLAREDLAFALSVILLGLLMMIARRNAVSQVIGFMS
                     IENGLILAATGAKGMPLVVEISVAFSILVALIVIGIFLFRIRERFETVDIHLLHEHRG
                     EQP"
     gene            complement(11410..12366)
                     /locus_tag="VIG70_09650"
     CDS             complement(11410..12366)
                     /locus_tag="VIG70_09650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEY8250860.1"
                     /translation="MAMILEAIVQLGQMIVVLALAPLLAGYVRTLKARLLGRRGPPLL
                     QPYRDLLRLLRKEVVLAHGASWVFRVAPYLVFATTWVAAALVPTFATGLLFSYAADVI
                     AIVALLGSARFMLALAGLDVGTSFGGIGSSREMMIASLAEPAMLMVVFTLSLLAGSTQ
                     LSSIAAFMQGPDVGLRVSLALALVALVMVAIAENARIPVDNPATHLELTMVHEAMILE
                     YSGRHLALIEAASFVKLLLYLSVIGCAFAPWGIAVAGQGFAAYLLGLAVYGAKLAAGG
                     FLLAVFETAIAKMRVFRVPDFLGAALMLGLLATLLLFVSRVA"
     gene            complement(12357..14369)
                     /gene="hyfB"
                     /locus_tag="VIG70_09655"
     CDS             complement(12357..14369)
                     /gene="hyfB"
                     /locus_tag="VIG70_09655"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEY8250861.1"
                     /translation="MVLEGLLWCVAALLGTGAAAVAIARRRTATALVYGISLCVTTAA
                     LLAAGAALLAPASLGGTLVLPIGLPWLGAHFRLDPLAAFFLVVVNLGAAAASLFGLGY
                     GRHEESPGRVLPFFPAFLAGMNLVLLAADAFTFLFAWEFMSLSSWALVMAHHRRPGNA
                     AAGYLYLLMASLGTLALVLAFGLLAGPSGGYAFEAMRQAPPAATVAGVVLVLALIGAG
                     SKAGLVPLHVWLPLAHPAAPSHVSALMSGVMTKVAVYGFVRIVFDLLGPPSWWWGALV
                     PLLGGVTAVMGVLYALMEHDLKRLLAYHTVENIGIIFIGLGLALAFEANAMPAAAALA
                     LTAALLHVFNHSVFKSLLFFGAGAVLGATGERDMERLGGLIHRMPLTAFAFLVGCAAI
                     SALPPFNGFVSEWLTFQAILLSPALPQWLLKFLVPAVGALLALSAALAAACFVKAFGI
                     TFLGRARTPAAGEAAETDRWSLAAMFTLAALCLLAGILPGYFIDALAPVIQPLTGTNL
                     PRQNELDWLTIVPIAQSRSSYNGLLLCLFIALAAGLGAEVIHRWASSASRRSDAWDCG
                     FPDASPATQYTASSFSQPIRRVFGTVVFRASEEVHIPAPGDARPARLHVRLRDVPWDT
                     LYVPAAGLVGYAADRLNRLQFLTIRGYLTLVFGALVALLMVLSVWQ"
     gene            complement(14370..14621)
                     /locus_tag="VIG70_09660"
     CDS             complement(14370..14621)
                     /locus_tag="VIG70_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250862.1"
                     /translation="MITSRQLRAARALAGVDRRALAKAARLSLPTIQRMEASDGVVRG
                     NVESLMKLIAALDRLGIELINEGAASESGGRGIRLKKRS"
     gene            complement(14677..15357)
                     /locus_tag="VIG70_09665"
     CDS             complement(14677..15357)
                     /locus_tag="VIG70_09665"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY8250863.1"
                     /translation="MSLPRPDYLIVSCEHGGNRVPSPFSKLFSRRFLDTHRGYDPGAL
                     AVARDFAAATGAPLFYSTVSRLLVELNRPLGHPQLFHLPLPEPVREALLRRYYFPYWN
                     AVHKAAQRGRRVLHLSCHSFTPKFRGVRRATDIGLLFDPARPAEAAFCRRWRRAILEE
                     DPHLRVRYNDPYPGVFPSLVESLRRKLGGRRYVGIQIEVNQKFPRGDARRWRALRRAL
                     VAAFTAVA"
     gene            15357..16256
                     /locus_tag="VIG70_09670"
     CDS             15357..16256
                     /locus_tag="VIG70_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015422050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEY8250864.1"
                     /translation="MPYSVEGKLVVAISSRALFDFEDENRVFERDGEPAYIALQYARL
                     DVPAREGVAFPLVKKLLAFNTPGQSRVEVVVLSKNDPVSGLRVFRSAKVAGLNIERGV
                     FTRGRSPYHYLEPLKANLFLSANETDVMGALEAKVPAARVYPESAQAASRHADEVRIA
                     FDGDAVLFSDEAERIYQRDGLDAFTQHETARALQPLPPGPFKPLLEALHRLQSEKNSS
                     VRIRTALVTARSAPAHERAVRTLMEWNIAVDEAMFLGGLDKGAFLKSFEPDFYFDDQH
                     GHVDSARVYVAAGHVPFGVANRR"
     gene            complement(16259..17248)
                     /locus_tag="VIG70_09675"
     CDS             complement(16259..17248)
                     /locus_tag="VIG70_09675"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8250865.1"
                     /translation="MDRRRAILALASCSGALAVAPALSQPAKPRRIGLLSPLGQGDPQ
                     TQSRLAAFEQELGRLGWKKGADVLLDYRFADGHATRAGQLAKELLDLHPEVVVAFGTS
                     SAAALRQQTLSIPIVFAQVVDPVAAGFVTNLARPEGNITGFTQFDSSIGEKWLQAIKE
                     CAPKVNRVALVFDPTNPAWTAHVRAVESAAKKFAVKLVPVSAKDAADLEKQITSFAAS
                     PNGALVIVPGAVTTTHSKVIVSLAARHRLPAVYPYSFHTANGGLMSYGVDLLSLYRGA
                     ASYVDRILKGAKPSDLPIQQPTRFELTVNLKTARALGITVPQSLLQQADKVIQ"
     gene            complement(17252..17659)
                     /locus_tag="VIG70_09680"
                     /pseudo
     CDS             complement(17252..17659)
                     /locus_tag="VIG70_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            17927..19267
                     /locus_tag="VIG70_09685"
     CDS             17927..19267
                     /locus_tag="VIG70_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250866.1"
                     /translation="MRRIAALLLAGMLVSCGGGGGGDDASNNSNSNSNSNTSTNTNTN
                     TNTSSSTPASVPGRYEESHAAVSLSTGWTQSDTGSFAWSGGAAVQSTVPGAHAVFTFT
                     GTSVAWIGTRNVNSGIALVKVDGGPGVEVDLFARPVVIHTPIITLNGLSPGSHTLTIE
                     VTGRQNPEALSSVVQVDAFDVQAPVVSQLQETDPDVIFSAGWVQADTNMAWSGGGVAT
                     LPDPPVGGARVTETPGANVTLTFRGTSISWSGYRGPNAGIARVSLDGVVSEVDTFSPA
                     DRVQAIVFTATGLADASHTLVIEATGRKNDASTGAQIVVDAFDVTTPGRRYQETDAAV
                     TYSGAWVHGNVNRTWSEGAASTSASAGARVTFTFTGTTVSWIGCRKLSTGLARVFLDG
                     VLVREIDTYLPPPIEGYQHTVFRADGLTNGAHTLTIEGASDGAFIVVDAFDLRP"
     gene            19642..21624
                     /locus_tag="VIG70_09690"
     CDS             19642..21624
                     /locus_tag="VIG70_09690"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEY8250867.1"
                     /translation="MQQIRIASQIGRCAARVLRIGLLALGFGAALALPAGAQAQGSDK
                     FIPTFLVYYGGGPQLVATDAAKLAKFDLIDIDRFRYQDIGPNTWAAIKALNPALQIYL
                     YEMGPEAPSHLDGTLPLFLNGLGRYDVSRGHFMGSLNGNHPGLFLLNNAGQRVYSVGF
                     SNIGANQYWHLMDFGDSSYQSYWIYAVKADIVDQPWAADGVFVDNCLTLANAGGYSAT
                     NTRYPTNTAWSAGMNSFVEAISNGMRAYGQKLWCNRGETRSVDGSAAWLSLDNGASPP
                     DVLLEEGAFAVEWGSDTQFYPELDWKRQIDTIAAIRNSKVATMSHTKLMDGQSGTDNW
                     GRPVSFAQTLWYALGSFLLAKNDELGNAYFMFNGGSGYNRIWWFDEYDNIDLGRAVGG
                     YRVTSFGGVNIYWREFERGYVYVNPTANNVASLALPQAARLLARDTLGALPELLPLVD
                     SVALAGHHAAFVLKADAGAPPAPDTTAPTVPAGLTAAAVSGTQVNLAWSASTDNVGVT
                     GYYVYLNDAPLAATSSTSFSHTGLTAGATYSYRVSAFDAVPNHSAWTATPVAVTTRVP
                     DTTRPNVSITSPSWNAIVSGTITVRASASDNVGVVGVQFKYNGASIGPEDTSAPFRAA
                     VVTTSMPNGTYALTAVARDAAGNTRTSAPVTIRVRN"
     gene            complement(21672..22817)
                     /locus_tag="VIG70_09695"
     CDS             complement(21672..22817)
                     /locus_tag="VIG70_09695"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050825 - ice binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HEY8250868.1"
                     /translation="MSRSGSFFKPLILLLAAFVAGCGDQSPGQVSSTSAATAAATMPP
                     LGTEQSFAVLGGSTVTNTGSSVITGNLGVSPGSAITGFPPGIVTGVIHAADAEALQAQ
                     TDTTAAYIDLASQACTSDSTGQDLGGKTLVPGVYCFSSSAQLTGALTLDAGGVSNAVW
                     VFKAGSTLTTAVNSSVVLINGANPCNVFWQVGSSATLGTTTSFVGNIFALASITLNNG
                     ASVSGRVLARNGAVTLDNNIVTVPACDVPVTSTPPTLSKTFSPGTINAGGVSTLTITF
                     SNANDTPANLTGPLIDTLPSGLVVVAGSVSTTCIETPLLRAWFIVDTSRVGLTAEGLI
                     PAKGSCTVTANVTAPVAGSYRNSLPAGALQTDHGNNAAPAVATLTVI"
BASE COUNT         4251 a         7710 c         7923 g         3535 t
ORIGIN      
        1 agccaacgcg cttgccaagg tcagaaaact gatcaactcc aaaaagcatc gcgtgccact
       61 tgaacgcgca aagctcctgc ccccgatctc caatccctgc ctgctgctct cggtcggcat
      121 gaactaccac gagcacctga aggaaatgaa gacaccggcg ccggaaaatc cagccgcctt
      181 cacgaaaagc gtggcgagca tcatcgccag cggccagccg atcaaagtcc cttcgtccaa
      241 cccgagcatg ctcgactggg agggcgagtt cacggtggtg atcggcaagg gcggctatcg
      301 catcccggca gcaaaagcgc tcgatcacgt cgccggctac accatggtca acgacgtctc
      361 ggcgcgcgac tgggtggcgg cgatcttctc ctcgaccggc gtcatgggcc cgatccacgc
      421 gtgggagcac aacctgctcg gcaagatgtt ccccaccttc tgccccatgg ggccgtgcat
      481 cgcgacccgg gacgagatcc ccgacccggc gaacgtgcgc atccagacgc ggctgaacgg
      541 ccatgtgatg caggacgcga ataccgacga cctggtgttc agcgtcgtga agctgatcga
      601 gtactactca cagttctacc gcttccagcc cggcgacgtc atcaccaccg gctcgccgag
      661 cggcgtcggc ttcgggcgca atccgaaggt gttcatgaag cccggcgacg tggtcgaggt
      721 cgaagtgaaa gagatcggcg tgctccgcaa ccccatcgag gcctaggcaa atggaattcg
      781 gctacttcac gctcagcgac aaccactacc cgaacaaccc gcgctcggcc aaccagttgg
      841 tgctcgagat ccgcgagcag gcgctgctcg ccgacaagat cggcatgcac tcggcgtgga
      901 tcggcgagca ccacttcgat tctctgggcg tcaactcgcg cccagacttg gttttggcga
      961 atattgcttc cgtcaccaag aacatacggc tcgcccccgc cgttacggtc ttgccgttgc
     1021 accacccggt gcacgtggcg gaaatgtggg cgacgctcga cgtgctctcc ggcgggcgcg
     1081 tcgacttcgc caccggccgc ggctacgacc gcaaggagta cgccccgttc ggcgccgact
     1141 tcatggcctc cgcggagatt ttcgaggaag gcatcgatgt gctgctgaag gcctggcgct
     1201 cgccggacaa gtggtcgcac cgcggccgct tctacaacat cccggagatg gcgatcacgc
     1261 cgagcccgct gcagaagccg ctgccgttct atgtcgcctc gttctcgaag accagcgtgg
     1321 acatcgccgc caagcgcggc ctcaacatca tctacgcgcc gttcgccgcc ggcatggtgt
     1381 tcggcggcct cgacaaagcg gtcgagacct accgcgaagc ctgcgcgaag gcgggtaaca
     1441 agcccggccg ggcgatgtgc agctacttca tctacatcgc cgacgacgcc aggcaggagg
     1501 actacggccg gcagacgcag atggactact tcaaccactg cgtgctgcag gccttcccgc
     1561 agaagaagga ggacgcgccg ccgaccatgc agtacttcat gaagatcgtc gacatcctca
     1621 agaacatgaa aaaggaggac ctgtcggaca aatccatcct gctcggctcg ccgcagaaga
     1681 tcgtcgacac cctgaagcgc gtcgagaaga gcggcatcga cgaggtgatc ctctacttca
     1741 acgtcggcaa caagccgcac aacctggtga aggagcagat gcaccgcttc atgcaggaaa
     1801 tcgcctccca gttcgagcgc aagcctgtgg cgtacggaac cctgagttag acaaaccgtc
     1861 gttcccgcga atgcggggac gactagactt tcctagatgc cgcccgaaac gtcgagcacc
     1921 gtgcccgtgg tgtaggaagc ggcgtctgag gcaagccaca ggatggcgcg cgcaacttcc
     1981 tcgggctgcc cggggcgctt catcggcgcg acatgctcgg cctttttcac gcgctccggg
     2041 tcgccgccgt cggcgtggat ctcggtgtcg atgagccccg ggcgcaccgc gttgacgcga
     2101 atcccttcgg tcgcaagctc gcgcgcgagg ccgccggtga acacctcgat cgcgcccttg
     2161 gaagccgcat agtcgaggaa catccccggc gcgcctgatt ttacgaaacc ggtggacacg
     2221 ttcacgatcg cgccgccgcg cccgccgtgg cgcacggaca tgcgtttgac cgcctcgcgc
     2281 gcacaaagca tcgcgccgaa gacgttggtc gagaagatcc gctggatcct cgccaggtcg
     2341 aaattttcga gccgcgacgc gggcgcgagg atgccggcgt tgttgaccag cacatcgatg
     2401 cgcccgagat cgcggtcgac ggtttcgaat aggcgctgca cgtcgcgttc gtcggcgacg
     2461 ttggcggcga cggcaatggc ttttcccttg aggcttttca ccacggcgtc tgcggcctgc
     2521 ttgttcttca ggtagttcac gcacaccgcg tagccctgct cggcggcgag ccggcaagtg
     2581 gcggcgccga tcccgcggct gccgccggtc acgaggatga tgttgctcaa gctaaggaag
     2641 gtctgaagaa gtcgtccccg cgaaggcggg gacccagtct agaaaattag gaagcctagt
     2701 cagaaatgtc tttccactcg ggattcatcc cctcgcttaa ctgggtcccc gccttcgcgg
     2761 ggacgaccat ttagcccttt ttgacgggcg gcgcgaccgt gcgcttgagc ttgtaggcag
     2821 tctgcttcgt aatgagcggc ttcatcgcgg cgaggaaggt ctgggtgtgc ttgcggccca
     2881 tgtggtcctg gaacgccgcc ggattgtcgt acacctcgta gagcacgatg cggctcggat
     2941 tgtcctcggc ctgcaggacg tcgaactgcc ggcagccggg ctcgtcggcg accgaggccg
     3001 cggcgttgac cgcgatcagc tggttgaact gcgccaggtg ctccggcttc acgtcgaact
     3061 ccacgatcag gacgaacttg ttcaagacgg ctccttcacg atgaaatcca tttcgaccag
     3121 cgcgccgcgc gcgagcgcgc tcacgccgat gcagctgcgc gcgggctggc gggcgcccga
     3181 gaagaacgcg gcgtacgcgc ggtccaggcg ctcgtggaag cgcttgaact ccaccaggtg
     3241 gatgcgcacc gacacgacgt gctcgcgcgc gaggccgcgc tttttcagga gggcctcgag
     3301 gttcgacagg atcctgtgcg cctggtcctc gaccgcttcg ggcagcgcct tcgcggcgtt
     3361 ctccaggtcg accgggccga gacccgagat gaagccccag ccgccggccc aggcttcact
     3421 gctcggcatg ctcgaacagg ccgcggatgc gccgcgcgac ttcgagctcg cgctccttgg
     3481 gcgggaggtc gtcggtctcg acgtacgcca tcgccgcgcg ccggccgaac tcggcgagcg
     3541 ccttcggatc ggacagcgcg ccgggcgagg gcacgcgcac cacggtctcg tgcgcgtacg
     3601 ttcgcacgcg agcagtcttc ggatagagac gcggcatcac gggcgtaccg cctttctcca
     3661 ccacgccgat cgcggaggtg agcatgcgcc gtgtcagcgc cacgccgcgg tcggtggtcg
     3721 cgagatgctc ggccgcgcgg ttggcgatgc gtccctggct cgtgaccgcg tcgtagtcgc
     3781 ccggctcgcg ctggcgctct tcgtagggcc ggtcgccggt ctggccgatg aacgggatca
     3841 tgtcgacgcc gaactgctcg ggcttgagcg agccttgcgg attgttgtac gaattgagat
     3901 gcgccacgcc gatgtacagc gagtgcgtgt cgtcgagcgg caccgcccag cgcgtgaggt
     3961 aggggcgcac gccgaaggtt tccctctgcg ccatgttcat gccgttgggg aactggccga
     4021 cgttcggcag catcatgtcg ctcgcgcgga tgaagacgta atcccctacc ttgcgcgtcg
     4081 ccatggacag gtagcccatc ggcgtctcgg ggaaatcgag ctgcggcagc accttgaaca
     4141 ccggcgagaa ctgctcgccg ctgacgatcg cgtgcaggaa ggcgttgtgc atcgggtcgg
     4201 cgccgttgtc gatgatctgc agccagttgc acggcaggtg catgttgaag gcgacgagct
     4261 ggttgtcctc cggatactcg aagctgtcgt agacggggaa ttcgggcagc gattccggcg
     4321 gccccatgta cgcgaagaga aggccgtagg cctcgcgcaa ggggtaggcg ccctggacga
     4381 tccggctctt gatccggtcg gtcggctcgg cgggcgtgtc gagaatcgtg ccgtcgacgt
     4441 cgaactgcca gccgtgatag cagcagcgga tgccgcgctc ggcgggaatg ccgaactcga
     4501 gcgaggcgcc gcggtgcgcg cagtgccggt gcagcaggcc gatcctgccc gactggtcgc
     4561 ggaaggtcac caggtcttcg ccgagcagcc gcacggcgac cggcaggtct ttcagctcgg
     4621 aggcgagcat gaagggatgc cagtagcggc gcaggtactc gccgcacggc gtccccgggc
     4681 cgacccgggt cagcaagtgg ccctcatcct ggggcggacg gttgtagtac ccgctgtaag
     4741 gagcgttggt gcgcatgcca gcctccggat cgacgcggcg agaataggag catcgattga
     4801 tcgagtcaat cgtttgattt agtcttcgcg cgtgagctcg gtgctggccg cagacctcgg
     4861 gcgcggtgcg caggcgcgcc tcgcgctggt ccacgcagcg gtgcaggtgt tcggcgagag
     4921 cggcctcgag gccgcgacca cgcgcgacat cgcgcagcgc gcgcagcaga acatcgcggc
     4981 gatcgcctac tacttcggcg gcaaggaagg actgtaccgc gcggtcgtcg agcacatcgt
     5041 ggaagtgatc ctcgcgcgcg tcggcccgct gatggacgag gccgaggcgt tcctcgagcg
     5101 cgagcagcag ccctccgagc gctgcctcga ctatctcaag cggttgctgg cgggctcgat
     5161 cgcgaccaac cagagcatga tcccggtgac cggcatcatc gtgcgcgagc agacccagcc
     5221 cacgcgggcg ttctcgatcc tctacgacgg ctgcctcgag cggctgcagc gcctgggcgc
     5281 cgggctgatc cgcgcctacc tcggcgaggc cgtgccgcgc gccgactgca tcgtgcgctt
     5341 ccatgccctg ctcggtgaat cgctcgcctt ccgcttcgcg cgcgagacca tcctgcgccg
     5401 caccggctgg aaggaaatcg gagcgcccga ggaagctcgc atccagtcca tcgtgaccga
     5461 gcacgcggag ctcgtgctgc gcgggctgag gcgcaagacc cgcaggtaac ctctaggagg
     5521 aggagccgtg aaccgctatc gcatccttgc acttgtgctc gccctgtgcg cgccggcatt
     5581 cgcctcggcg caggcattcc cgagcaagcc gatccgcatg atcgttccct atgcgccggg
     5641 aggcgcgacc gacgtgctgg cgcgtccgat cgcgcagcgc ctgagcgagc aggtcggcca
     5701 gccggtggtc gtcgagaacc gtcccggcgc caacgccacc atcggcgccg accacgtcgc
     5761 caagtcgccg ccggacggct acaccttctt cctgggcagc atcgtgcact acatggtgcc
     5821 cttcttcagc aagaacgtgc cctacgatcc ggtgaaggac ttcacgccgc tcgcgctggt
     5881 ggcggtggtg ccgaacatcc tcgcggtcaa tccctcgctg ccgatccact cggtgaagga
     5941 cctgatcgac tacgcgaaga aaagcggcgt caagatgcac tacggcacga ccggcaccgg
     6001 ctcgacgcac catctgggcg gcatcttcct cgcgcagacc gcgggcatcc cgctcgaaca
     6061 cgtcccctac aaaggcggca acccggcgat ccaggacgtc ctgtcgggct cgattccgct
     6121 ggtgatcctc acggccacga ccgtcatgcc gcaggtgaag aacggcaagc tgcgcgcgct
     6181 cggcctgatc gagaaccggc gcttcgacgt ggtgcccggg gtgccgacca tcggcgagag
     6241 cctgccgggc tatgcggtgc cggacacctg gttcggcgtg ctgggccccg ccggaatgcc
     6301 gcgcccgatc gtggagcggc tgaacagcga gctgcgcaag gcggtgcagg ctccggacgt
     6361 gcgccagcgc atcgagaaca tcggcatggt gccgaccggg acggcgggca tcgacgaaat
     6421 cgccgccaac atcgcgagcg aggtcgacac gatccgcaag atcgtgaccg ccgcggacat
     6481 caagcctgaa tgaagctcgc gaccttccag gagggcggcg catcgcctgc cgtcggcgca
     6541 gtcgagggcg acgtcatcgt cgatctttcg gggcattttc cgagcatgct ggcgctcatc
     6601 gatggcggcg aggcggcgct cgagctcgcc cggaaggcgg tgaagcgctc ggccgtgaag
     6661 aagaagctgt cgcaggtcag gctgctgtcg ccggtgcccg agccgcgcca gatccgcgac
     6721 ttcaacaatt tcgagcagca catccgcaac gcgccctggg gcgtggccaa gctgcgcgcg
     6781 cgcctcgccg gccagccgga gcccgatcgc agcaaggcgc cggcggtcgg cgagatcacc
     6841 ttcaagcagc cgatctttta cctcagcaac cgcttcaacg tcgtcggtca cgaggccgag
     6901 gtgcagtggc cgggctattc cgagtggctc gacttcgagc tcgagttcgg cgtgttcctc
     6961 ggccggaaag gcaagaacat cgcaaaggag cgcgccgccg agcacgtctt cggctatgcc
     7021 gtgttcaacg acttctcggc gcgcgaccgc caggtgcgcg agatggaagg ctggatgggg
     7081 ccgacgaagg gcaagagctt cgacaccggc aacgccatcg gcccctggat cgtcacgaag
     7141 gacgagattc ccgacccgca ggcgcttgcc gtcagcctgc gcgtcaacgg cgaggtctgg
     7201 gcaaaaaaca ccaccgccgg catgatccac agcttcgagg acatgatcag cttcgtctcg
     7261 cacgacgaga ccctgcaccc gggcgagttc ttcggctcgg gcaccatcgg cggctgctgc
     7321 ggcctcgagt gcgatcgctg gctgaaggac ggcgacgtgg tggaggcgga agtggagaaa
     7381 atcggggtgc tgcgtaaccg cgtcgtgcgc cgcaagcgct gactaggtcg aaacgctctc
     7441 gacgagggcg agcaggcctt tgagcaatgc gacgggcgac ggcgggcagc cggggatgtg
     7501 cagatcgacc cgaagcacgg ccgagactcc gccgacacag gcagcgctgc cggcaaagac
     7561 gcctccattg cgcgcgcagt ctccgaccgc cacgacccat ttcggatcgg gcgtagcctg
     7621 gtaggtgcgc tcgagcgcct cgcgcatgtt ggccgtgacc gggccggtca ccagcagcac
     7681 gtcggcatgg cgcggagagg cgacgaagcg caggccgaag cgctccaggt cgtagagcac
     7741 gttgttgagc gcatggatct cgagctcgca gccgttgcag gagcccgcgt cgacctcgcg
     7801 gatcgagagc gtgcgtccca gccgcgcgcg cgaggcctgg ttcacgcgcg cggcaagctc
     7861 ggccagcgcg gcctcgtccg ccggaggcgc gggctcggtg agcgggccgc cgacgaggtt
     7921 tctcagcaat actttagaga tcatggcctg aataggagca gttgaaggac ttattgcaga
     7981 gcgggaagtc ggcgacgatg ttgccttcga tcgccgcctc gagcagcggc cactggaacc
     8041 acgacgggtc gcgcaggtgg gagcgctcga cccggccgtc ggcgccgatc cggatccacg
     8101 caaagatgtc gccgcggaag ccttcgacga gcgccgtgcc ttccccttgc gcgggcgcga
     8161 tcggggaaaa taccggcccc ggcggcaagc gctcgaggat ctgctcgagc agcgcgagac
     8221 tctgctccac ctcctgcatc cgaatccaca cccgcgcgtt gacgtcgccc gcctgcacca
     8281 cgggcacctc gaactcgagc tcgccgtagg gcgcgtagcc cggcgcgcgc cgcgcgtcga
     8341 agtggcgacc cgaagcgcgt ccgacatagc cgccggcggc gtactgctgc gcgagcgcgg
     8401 gcgaaaggat tccggtgtcg acggtgcggt ccttgagcga ggcggtgttc tcgtacaggt
     8461 cgacgagctc ggggaagcgc ctggcgacga gggcgagcag cgcgcgcacc tcccgctcgc
     8521 cctgcgccgc gaggtcggcg cgcattccgc cgggaacgac gcaatccatc atcagacgat
     8581 ggccgaagca cgcattcgac gcgcgcagga cctgctcgcg cagcaggccg cagctcgcat
     8641 gcatgatgct gaacgacgcg tcgttgcaca ccgcgccgat gtcgccgaag tgattggcca
     8701 gccgctcgag ctcgcccgcg agcgccctca gccagagcgc ccgcggtgga acctgcgcgc
     8761 cgagcgcagc ctcgacggcc atcgagaagg caagcgcgta cgcgacggtg ctgtcgcccg
     8821 agacgcggcc ggccagcgcc gcggcgcgct cgagcggcgc acccgccatg agcgcgtcga
     8881 tacccttgtg cacgtagccc aggcgctcct ccagccgcac cacggtctcg ccgttggcgg
     8941 tgaagcggaa gtgacccggc tcgatgatcc cggcgtgcac cgggcccacg gggatctggt
     9001 gcaggccttc gccttccgcg ggcaggaacg cgtaggcgcc ggcgtgacgc agccagggcc
     9061 gcgtgtccgg cgcgccttca ggctccagcc cagcgaggtc gcgtaccgcg cgctcgagcc
     9121 ggatcgccgg cggatgcacg cggccgaccg aggggaagcg gttctcttca cacggcaaat
     9181 cgagcaaatg aattttgctt ttcatgtcga taagagccat gtgcgcgcgg cccggttctc
     9241 cccacaagct gacgagcgag cactcgccgg tgcgcaatcg ttcgatggct tgcgtccagt
     9301 tcatgtcagt tcaggagggt ggcgacgtgc tggaaccagg aaaccagcgc cggcggcagc
     9361 cagattcccg ccgcgagcac gaggacgagg tgcagtgcga ccggcaggaa cgaggccttc
     9421 gcgggatcgc ggcggccgtc cggatcgcca aacgccaggc cgttcaggcg caacagcagc
     9481 gcgcccagcg cgaccagcag ggatagcccc aggatgacgg cgagcgccgg ctcgcgcgcg
     9541 aacgtggtag tgacgatcag gaactcggtc atgaagagtc ccatcggcgg caggccggcg
     9601 atcgccacca cgcccaggga caggctccag ccgagcaccg ggtgcgacgc ggtgagtccc
     9661 cggatgtcgg ccatgcgctg ggtgcccttg acctgcgcga tatggccgac gcagaagaag
     9721 atcgcggatt tcgtcaggct gtgcagcgtc atgtgcagca gtccggcgaa gttggcgagc
     9781 gtgcctccca tgccgaaagc gaaggtcacg atccccatgt gctcgatcga cgagtaggcg
     9841 aacagccgct tgatgtcctg gcggcggtac agcatgaagg acgcgaacac caccgaggcg
     9901 aggccgagcg tcaccatcag cgggccgggc gcgagcgccg ccggattcgc cgacatgatg
     9961 ctcttgaagc gcagcaccgc gaacaacgcg acgttgagaa gcagcccgga cagcaccgcc
    10021 gagatcggcg tagggccttc ggcgtgcgcg tcgggtagcc aggcgtgcag cggcacgagc
    10081 ccgaccttgg tgccgtagcc gaggagcagg aacacgaacg agaggttgag gagcgcgggg
    10141 tcgaactgcg ccgcgtgctc cacgagcacg ttccaggcca tcgcgtcgag cccttcgccc
    10201 accaccgagc gggccgcgag gtatacgagg atcgtgccga agagcgcgag ggcgatgccg
    10261 acgcttccca gcaggaagta cttccacgcc gcctcgagcg cggcgggcgt gcgatagatc
    10321 cctaccatca gcaccgtggt cagcgtcgcc agctccaccg ccacccacat cagcccgatg
    10381 ttgttggaca gcaaggcgag gttcatgccg aacatcagca cctggtacat ggcgtggtag
    10441 aagcgcaggt aggcgggcgt gagctggccg gtctcgagct cgtgcgcgat gtagctcccg
    10501 ctgaagacgc tggtggtgaa cccgaccagc gtgctgagga cgatgaacac cacgttcagg
    10561 tcgtcgacga tgaggaaggg ccccggcgca ggacgctcga ccagcaggac gagcgcgcag
    10621 gcgagcgtca ggaacgagac cgaggcattg agccgcgcgc tgacgcggtc atccggcaga
    10681 agcgcgagca cgccagcccc gagcgcggga atcacgagga tcgcggccag caccagggag
    10741 cccccggtca tggctgctcg ccccggtgtt cgtgaagcag gtggatgtcg accgtctcga
    10801 agcgctcgcg gatgcggaag agaaaaatgc cgatcacgat cagggcgacg aggatcgaga
    10861 aggcgacgct gatttccacc accagcggca tgcccttggc gccggtcgcg gcgaggatca
    10921 gcccgttctc gatcgacatg aagccgatca cctggctcac ggcgttgcgg cgggcgatca
    10981 tcatcaggag gccgagcagg atcaccgaca gcgcgaacgc gaggtcctcg cgcgcgagcg
    11041 ttcccgcctg ctccgtgatc ggcagcatca ccatgatgga cagcgcgacc aggccgatgc
    11101 cggcgagcat cgtgagcccg acgccgacga ccggctcgat ggtgcggtga atccccaggc
    11161 gcaccacgat gcgatgcagc gcgaggggaa tcaccaccgc cttcacgacg agcgcgatcg
    11221 cggccgtgat gtagaggtgc ggggcgtcct ggatcgcggc ctgccaggcc accgagagcg
    11281 agacgaccag cgcgtgcagc gcgaacgtgt tgatcagccc ggtcatgcgg tcctggtaga
    11341 gcagcatgaa gctcatcagc accaggccgc cggcgaagag gtgcgcgacg tcgaacgcga
    11401 gctggccact catgcgacgc gcgacacgaa gaggagcagc gtcgccagca ggccgagcat
    11461 cagggcggcg ccgaggaaat ccgggacgcg gaacacacgc atcttggcga tcgcggtctc
    11521 gaacaccgcg agcaggaagc cgcccgcggc gagcttggcc ccgtagacgg ccagcccgag
    11581 gaggtaggcg gcgaagccct ggccggccac ggcgatcccc cagggagcga acgcgcaacc
    11641 gatgaccgag aggtagagca gcagcttcac gaacgacgcg gcttcgatca gcgccaggtg
    11701 gcggcccgag tactccagga tcatcgcctc gtgcaccatg gtgagctcga ggtgtgtcgc
    11761 cgggttgtcg accgggatgc gcgcgttctc cgcgatcgcc accatgacga gcgcgaccag
    11821 cgccagcgcg agcgacacgc gcaggccgac gtccgggccc tgcatgaacg ccgcgatcga
    11881 ggaaagctgg gtggagccgg ccaggaggga gagcgtaaag accaccatca gcatcgccgg
    11941 ctcggcgagc gaagcgatca tcatctcgcg gctcgagccg atgccgccga agctcgtgcc
    12001 gacgtcgagc cccgccagcg ccagcatgaa gcgggcgctg ccgagcagcg cgacgatggc
    12061 gatcacgtcc gcggcgtagc tgaagaggag cccggtggcg aacgtgggca ccagcgccgc
    12121 ggcgacccag gtcgtcgcga acacgaggta gggcgcgacg cggaataccc acgaagcgcc
    12181 gtgcgccagg accacctcct tgcgcagcag ccgcagcagg tcgcgatagg gctgcagcag
    12241 gggaggcccg cggcgaccga ggaggcgcgc cttgagcgtg cgcacgtacc ccgcgagcag
    12301 gggcgccagc gccagcacca cgatcatctg gccgagctgg acgatcgcct cgaggatcat
    12361 tgccatacgc tgagcaccat cagcagcgcc accagcgcgc cgaacaccag ggtcaggtag
    12421 ccgcggatcg tcaagaattg cagccggttg agccggtcgg cggcatagcc caccagcccg
    12481 gccgcaggga catacagcgt gtcccaggga acgtcgcgca gccgcacgtg caggcgcgcc
    12541 gggcgggcat ctcccggcgc ggggatgtgg acttcctcgc tcgcccggaa caccacggtt
    12601 ccgaagaccc ggcggatcgg ctgcgagaag ctgctcgcgg tgtactgcgt tgccggactg
    12661 gcgtcgggaa agccgcagtc ccacgcgtcg gagcggcggc tcgctgacga cgcccaccga
    12721 tggatcactt cggccccgag cccggccgcg agcgcgatga acaggcacag cagcaggccg
    12781 ttataggagc tgcggctctg cgcgatgggg acgatcgtca gccagtcgag ctcgttctgg
    12841 cgcggcaggt tcgtaccggt caggggttgg atcaccgggg cgagcgcgtc gatgaaatag
    12901 cccggcagga tgcccgccaa caggcacagc gcggcgagcg tgaacatcgc cgccagcgac
    12961 cagcgatcgg tctccgcggc ctcgcccgcg gctggcgtgc gcgcgcggcc gaggaaggtg
    13021 atgccgaacg ccttgacgaa gcacgccgcc gcgagcgcgg ccgacagggc gagcagggcg
    13081 ccgaccgcgg gaacgaggaa cttcaggagc cactgcggca atgccgggct gagcaggatc
    13141 gcctggaacg tgagccattc ggagacgaac ccgttgaacg gcggcagcgc ggagatcgcg
    13201 gcgcagccga cgaggaaggc gaaggcggtg agcggcatgc ggtggatcag cccgccgagc
    13261 cgctccatgt cgcgctcgcc ggtcgcgcca agcaccgcgc cggcgccgaa gaacagcagg
    13321 ctcttgaaca ccgagtggtt gaagacgtgg agcagtgccg cggtcagcgc cagcgcggcc
    13381 gccgccggca tcgcgttcgc ctcgaacgcg agcgcgagcc ccaggccgat gaagatgatg
    13441 ccgatgttct cgacggtgtg ataggcgagc aggcgcttga ggtcgtgctc catgagcgcg
    13501 tagagcacgc ccatgacggc ggtgacgccg ccgagcagcg gcacgagggc gccccaccac
    13561 cagctcggcg gcccgagcag gtcgaacacg atgcgcacga agccgtagac cgcgaccttg
    13621 gtcatgacgc cgctcatcag cgccgagaca tggctcggcg cggccgggtg cgcgaggggc
    13681 agccagacgt gcagcggcac gaggcccgcc ttggagcccg cgccgatcag cgccagcacc
    13741 aggaccacgc ccgcgaccgt ggccgcgggc ggcgcctgcc gcatggcctc gaacgcatag
    13801 ccgcccgacg gcccggcgag caggccgaac gcgagcacga gcgcgagcgt cccgaggctc
    13861 gccatcagca ggtagagata cccggccgcc gcgttgcccg gccgccggtg gtgcgccatc
    13921 accagcgccc acgaagagag cgacatgaac tcccacgcga agagaaaggt gaacgcgtcc
    13981 gcggcgagca gcacgaggtt catgccggcg aggaacgccg ggaagaaagg cagcacgcgc
    14041 ccgggcgact cttcgtggcg gccatagccg aggccgaaca ggctcgccgc ggcggcaccc
    14101 agattcacca ccaccaggaa aaaggccgcc aggggatcga gcctgaaatg cgcacccagc
    14161 cagggcaggc caatcggcag gaccagcgtg ccgcccagag acgcgggagc gagcagggct
    14221 gcacccgccg caagaagcgc ggcggtcgtg acgcagagcg agattccgta caccagcgca
    14281 gtcgctgtgc gacgccgcgc gatggcgacc gctgccgccc ccgtcccgag cagcgccgca
    14341 acgcaccaca acagcccttc cagaaccact cagctcctct tcttcagccg gatgccgcgg
    14401 ccgccgctct cgctcgccgc gccttcgttg atcagctcga tgccgagacg gtcgagcgcc
    14461 gcaatcagct tcatcagcga ctcgacattg ccgcgcacca cgccgtcgct cgcctccatg
    14521 cgctgaatcg tgggcagcga caggcgcgcg gccttggcca gcgcgcgccg gtcgacgcct
    14581 gcgagcgcgc gggcagcccg gagctgccgc gacgtgatca tacgtagagc attagttaag
    14641 atacataaaa tacactaaat gatacaaaat acatcatcac gccacggctg taaatgcggc
    14701 gacgagcgcc ctgcgcaggg cccgccagcg gcgcgcgtcg ccgcgcggga atttctggtt
    14761 cacctcgatc tggatgccaa cgtagcgccg gccgcccagt tttctccgca gcgattcgac
    14821 caggctggga aaaacgcccg gataaggatc gttgtagcgc acgcgcaagt gcggatcttc
    14881 ctcgaggatc gcgcgacgcc agcggcggca gaacgccgct tccgccgggc gggccggatc
    14941 gaacagcaag ccgatgtcgg tcgcgcgccg cacaccgcgg aatttcggcg tgaagctgtg
    15001 gcaggagaga tgcagcacgc ggcgaccgcg ctgcgcggcc ttgtggactg cattccagta
    15061 tggaaagtag taccggcgca aaagagcttc gcgcaccggt tccggaagag gcaggtggaa
    15121 cagctgcgga tgaccgagcg ggcggttgag ctcgacgagc aggcggctga ccgtggagta
    15181 gaagagcggc gcgccggtcg cggcggcgaa gtcgcgcgcg acggcgaggg ccccggggtc
    15241 gtagccgcga tgcgtgtcga ggaatctcct ggaaaacaac ttcgagaacg gcgagggcac
    15301 gcgattgccg ccgtgctcgc agctgacgat gagataatca gggcgaggga ggctcattgc
    15361 cgtactccgt cgaaggcaag ctggtggtcg cgatctcctc gcgggcgttg ttcgacttcg
    15421 aggacgagaa ccgcgtgttc gagagggatg gcgagcccgc gtacatcgcg ctgcagtatg
    15481 cccggctcga cgttccggcg cgcgaggggg tggcgttccc gctggtgaag aagctcctgg
    15541 cgttcaacac gccgggccag agccgcgtcg aggtggtcgt gctgtccaag aacgatccgg
    15601 tttcgggact gcgcgtgttc cgctcggcca aggtggcggg gttgaatatc gagcgcggcg
    15661 tgttcacgcg cggacgcagc ccttaccact acctcgagcc cttgaaggcg aacctcttcc
    15721 tgtcggcgaa cgaaaccgac gtgatgggcg cgctcgaggc caaggtgccg gcggcgcgcg
    15781 tgtaccccga atcggcgcag gcggcaagcc ggcacgccga cgaggttcgc atcgccttcg
    15841 acggcgacgc ggtgcttttc tccgacgaag ccgagcgcat ctaccagcgc gacggcctgg
    15901 acgcgttcac gcagcacgag acggcgcgcg ccctgcagcc gctgccgccg ggtcctttca
    15961 agccgctact ggaagcgctg caccggttgc agagcgaaaa aaattcttca gtgagaatca
    16021 gaaccgcttt ggtcacggcc cgcagcgctc cggcgcacga gcgcgccgtg cggacgctga
    16081 tggaatggaa catcgcggtc gacgaggcga tgttcctcgg cggcctcgac aagggcgcgt
    16141 tcctcaagtc cttcgagccc gacttctact tcgacgacca gcacggccac gtcgattcgg
    16201 cgcgcgtgta cgtggctgcg ggccacgtgc cgttcggcgt tgccaaccgt cgctagggct
    16261 actggatcac cttgtcggct tgctgcagca acgattgagg gacggtgatg ccgagagctc
    16321 gcgcggtctt gaggttgaca gtgagttcga accgggtcgg ttgctggatc ggcaggtcgc
    16381 tcggcttggc ccctttgagg atgcggtcga cataggacgc cgcgccgcga tagaggctca
    16441 aaaggtccac cccgtacgac atcagcccgc cattcgcggt atggaagctg tacgggtaga
    16501 ccgcgggcaa gcgatgtcgc gccgccaggg acacgatgac cttgctgtgg gttgtggtca
    16561 cggcgcccgg cacgatgacg agggcgccgt tcggcgaggc tgcgaagctc gtgatctgct
    16621 tctccaggtc cgcggcgtct ttcgcgctga ccgggaccag cttgacggcg aacttcttcg
    16681 cggcggactc gactgcgcgg acgtgcgccg tccacgcggg attggtcgga tcgaacacga
    16741 gcgcgacccg attgaccttg ggtgcgcatt cctttatcgc ctgcaaccac ttctcgccga
    16801 tcgaggaatc aaattgcgtg aagccggtga tgttgccctc gggacgagcg agattcgtca
    16861 cgaagccggc cgccactggg tcgacgactt gcgcgaacac gatcggaatg gaaagcgttt
    16921 gctgccgcaa tgccgccgcg ctggaggtac cgaacgcgac gacgacttcg gggtgcagat
    16981 cgagcagctc cttggccagc tggcccgcgc gcgtcgcgtg gccatccgca aaccggtagt
    17041 ccagaaggac gtccgcgccc tttttccagc ctagcctgcc cagctcctgc tcgaatgcag
    17101 cgagcctcga ttgcgtttgc gggtcgccct gccccaaggg tgagagcagt ccgatacgac
    17161 gcggctttgc cggctgcgat agcgcaggcg caacggcaag cgcgccgctg caggaggcaa
    17221 gcgccaggat tgctcggcga cgatccatgg gttagatcct gccttcctcg acggcgtggc
    17281 aggcgacgcc gtcgctgaac ggcggcacct cgacctggca gcgcgcgttg gcgtgcgggc
    17341 agcgtggatg gaacgggcag cccgcgggcg gggcgagcga atttggcacc tcgcccgcga
    17401 ctggcgtgcg cggcttgccg gtcatggcga ggtcgggcac ggcgtcgagc agcatgcggg
    17461 tgtagggatg gcgcgggcgc gcgaagaggt ctgacgcatt tgccagttcc acgatgcggc
    17521 caaggtacat gacgccgacg cggtcggcta ccagcgacac cacggcgagg ttgtgcgaga
    17581 tgaagaggta ggtgaggccg aggcggtcct ggaggtcggc catcaggttc aggatctgcg
    17641 cctgcaccga gacgtcgagc acggtgatcc accattcgag caagggctgg gtggcgggca
    17701 cgcccacccc caggaacgag agcgtggcct cggtgatgat cgccgtggcg atgtggatcg
    17761 tagcaatcac caccggccgt cccgacggcc tagttcccgg ctggcgcgcg ccgcgaggcc
    17821 gcgaacgtcc atacgcatca tcctgccgcg caggaaaatc atgctgataa ccgagactct
    17881 ccccaaagac acggaaagcg cggttatgta cggagcagtt caagaaatga ggcgcatcgc
    17941 agccttgctc ttggcgggca tgctggtttc ctgcggcggc ggtggcgggg gcgatgacgc
    18001 cagcaacaac agcaacagca acagcaacag caacacaagc accaacacaa acaccaacac
    18061 aaacaccagc agcagcacgc ccgcatcggt gcccgggcgc tacgaggaat cacatgcggc
    18121 agtaagcctg agtaccggtt ggacgcagag cgacaccggc tcgtttgcgt ggagcggcgg
    18181 agccgccgtg caatccacag tgccgggagc gcatgcggta ttcacgttca ccgggacctc
    18241 ggtcgcgtgg attggcaccc gcaacgtcaa tagcggaatc gccctcgtca aagtggacgg
    18301 gggtcctggc gtcgaagtgg atctgttcgc gcggccagta gtaatccaca cgcccatcat
    18361 cacgctgaac ggcctgagcc ccggcagcca cacgctgacg atcgaggtga cgggccggca
    18421 gaacccggag gccctgtcca gcgtcgttca ggtggatgcc ttcgacgtgc aagcgccggt
    18481 ggtctcgcag ttgcaggaga cggacccgga cgtgatcttc agcgccggct gggtccaggc
    18541 ggacaccaat atggcatgga gtggcggcgg cgtggccacg ctcccagatc cgccggtcgg
    18601 gggcgcgagg gttaccgaga cgccgggcgc gaatgtcacg ctgacgttcc gcggaacatc
    18661 gatcagttgg agcggctatc gcggccccaa tgcgggcatc gcccgcgtgt ccctcgatgg
    18721 cgttgtcagc gaggtcgaca cgttctctcc ggcggacagg gtccaggcca tcgtattcac
    18781 ggcaaccgga ctggccgacg cgagccacac gctggtgatc gaggcgacgg gccgcaagaa
    18841 cgacgcgtcg acgggcgccc agatcgtggt ggacgccttc gacgtgacga cgccgggaag
    18901 gcgctaccag gagacggatg cggcagtcac ctacagcggt gcttgggtgc acggcaacgt
    18961 gaacagaacc tggagcgagg gagccgcgtc cacatccgct tcggcgggcg cgcgagtcac
    19021 cttcaccttc accgggacca cggtcagctg gatcggctgc cgcaagctca gcacggggct
    19081 cgccagggta ttcctcgacg gagtccttgt gagagagatc gatacctatc ttccgccccc
    19141 gatcgaggga taccagcaca ccgtcttcag agcggatggg ctgaccaatg gcgcgcatac
    19201 actgacgatc gagggtgcga gcgatggtgc cttcattgtg gtggatgcct ttgacctccg
    19261 cccatagggc aggcgaccca agacaggcct gccgaagcag gcgagcggga agggcggcta
    19321 gaggaggaag ctagccccgc gccccgcgct cgccgccgcg gtccttgaat tcgaaagcga
    19381 acactacgta gcgctgcggc aatggccggc ggcacgagcc aggcggcgag aatgatgccg
    19441 actgtcgccg cgcggctaaa aagtgtcgcc gccgcacgtc ggattgctgt tcgcgcgtcg
    19501 attccttcac ataactgctc agggaattcc tgagttcatg cgaaacgtcc tcgttggccg
    19561 tgcgccgcgc ccctagcatg cgacccgttt gtagaacgca ggcgacagac gccgcatttc
    19621 tccactacgg ggaagcacag catgcaacag atccgcatcg catcgcaaat tggcaggtgc
    19681 gcggcgcgcg tgctgcgcat cggcctgctc gcgctgggtt tcggcgcagc cctcgccctt
    19741 cccgcgggcg cgcaagccca aggctcggac aagttcatcc cgactttcct ggtgtattac
    19801 ggcggcggcc ctcaactggt ggccaccgat gcggcgaagc tcgccaaatt cgacctgatc
    19861 gacatcgacc gctttcgcta ccaggacatc ggcccgaaca cctgggcggc gatcaaggct
    19921 ctcaatcccg ccttgcagat ctacctctat gaaatggggc cggaggcgcc gagccacctc
    19981 gacggcacgc tgccgctgtt cctgaacggg ctggggcgct acgacgtttc gcgcggccac
    20041 ttcatgggca gcctgaacgg caatcacccc gggctgttcc tgctgaacaa cgcgggccag
    20101 cgcgtctaca gcgtcggctt ctccaacatc ggcgccaacc agtactggca cctgatggat
    20161 ttcggcgact ccagctatca gtcctactgg atctacgcgg tgaaggccga catcgtcgac
    20221 cagccctggg cggcggacgg cgtgttcgtc gacaactgcc tcacgctcgc caacgcgggc
    20281 ggctacagcg cgaccaacac caggtatccc accaataccg cctggtcggc cgggatgaac
    20341 agcttcgtcg aggcgatcag caacggcatg cgcgcctacg gccagaagct gtggtgcaac
    20401 cgcggcgaaa cgcgctcggt ggacggcagc gcggcctggc tgagcctgga caacggcgcc
    20461 agcccgcctg acgtgctgct cgaggaaggc gctttcgcgg tggaatgggg ctccgacacc
    20521 cagttctacc ccgagctgga ctggaagcgg cagatcgaca ccatcgccgc gatccgcaat
    20581 tcgaaagtgg ccaccatgag ccacaccaag ctgatggacg gccagagcgg cacggacaac
    20641 tgggggcggc cggtgagctt cgcgcagacc ctgtggtacg cgctgggctc gttcctgctc
    20701 gccaagaacg acgagctggg caatgcctac ttcatgttca acggcggcag cggctacaac
    20761 cggatctggt ggttcgacga gtacgacaac atcgacctgg gcagagccgt aggcggctac
    20821 cgcgtcactt cgttcggcgg cgtcaacatc tactggcgcg agttcgagag aggctatgtc
    20881 tacgtcaatc cgaccgccaa caacgtcgcc tcgctcgcct tgccgcaagc cgcgcggctg
    20941 ctggcgcgcg acaccctcgg cgcgctgccc gagttgctgc cgctggtgga tagcgttgcc
    21001 ctcgccgggc accacgcggc gttcgtcctg aaggcggacg cgggcgcgcc gcccgcgccg
    21061 gacaccaccg cccccaccgt tcccgcgggt ctcaccgccg ccgcggtgtc cggaacccag
    21121 gtgaacctcg cctggagcgc gtccactgac aacgtcggcg tgacgggcta ttacgtctac
    21181 ctgaacgacg cgccgctcgc cgccaccagc tcgacttcgt tctcgcacac cggcctcacg
    21241 gccggcgcca cctacagtta ccgcgtgagc gcgttcgacg ccgtgccgaa ccactcggcg
    21301 tggacggcaa ccccggtggc ggtgacgaca cgcgtgcccg acacgacgcg cccgaacgtc
    21361 agcatcacct cgccgtcgtg gaatgccatc gtctccggca ccatcacggt gagggccagc
    21421 gcctccgaca acgtcggcgt ggtgggcgtg cagttcaagt acaacggcgc cagcatcggc
    21481 cccgaggaca ccagtgcgcc cttcagggcc gccgtggtca cgacgtcgat gcccaacggc
    21541 acctatgcgc tgacggcagt ggcgcgggac gcggcgggca atacgcgcac ctcggcgccg
    21601 gtcacgatcc gggtgcgcaa ttgagcatgg catagcccgg ccgccggccc acgaggtctt
    21661 agtgactgcc gctagatgac ggttagtgtc gcgacggcgg gggcggcgtt attgccatgg
    21721 tcggtctgca aggcgccggc aggaagcgaa ttgcggtagc tgcctgcgac cggcgcggtc
    21781 acgttcgccg tcaccgtgca ggagcccttg gccgggatca ggccttccgc cgtcaagccc
    21841 accctcgagg tgtcgacaat gaaccacgcc cgcaagagcg gggtctcgat acaggtggtg
    21901 ctgacgctgc ccgcgaccac caccaggccg cttggcaatg tgtcgatcaa cggcccggtc
    21961 aggttggcgg gtgtgtcgtt ggcgttacta aaggtaatgg taagtgtcga gaccccgccc
    22021 gcattgatgg tgcccgggct gaaggtcttg ctgagcgtcg gtggggtgga agtgaccggt
    22081 acgtcgcatg ctgggacggt gacgatgttg ttatccagcg tcacagcacc gtttcgcgcc
    22141 aggactctgc cggacacgct tgcgccgttg ttgagggtga tgcttgcgag cgcaaaaata
    22201 ttcccaacga acgaagtggt tgtaccgaga gtcgcggagc tgccgacctg ccagaagacg
    22261 ttgcacgggt tggcgccatt gatcagcacc acggaggagt tgaccgcggt cgtgagggtg
    22321 ctccccgcct tgaacaccca gacggcattg gagacgcccc cggcgtcaag cgtgagcgca
    22381 cccgtcagct gggccgagga tgaaaaacaa tagacgcccg gcacgagcgt cttcccgccg
    22441 agatcctgac cggtcgagtc ggaagtgcac gcctggcttg ccagatcgat atacgcagcg
    22501 gtggtatcag tctgagcctg aagcgcctcc gcatcggctg cgtgtatcac cccggtcacg
    22561 atcccgggcg gaaagccggt gatcgccgag cccgggctga ctcccaggtt tccggtgatg
    22621 acgctggaac cggtattcgt caccgttgaa ccgccgagca ccgcaaagct ctgctccgtg
    22681 cccagtggtg gcatggttgc ggccgcggtc gccgccgaag tcgagctgac ttggcccggg
    22741 ctctggtccc cgcatccggc cacaaaagca gccagcagta gtatcagtgg tttgaaaaag
    22801 cttccggatc tggacatgtg tttctcctgg gcactgcagg ccgaaaggga tcggcgtgcg
    22861 gctgatgcat tgcggccgcc aatccgtgcg cacggcgctg aaggtcttgc ggggttttgg
    22921 ccccgggcac tggctacttt ccccctccgc cgcaaaacgt tctgtacgat tgcgcacgca
    22981 atccaagaaa atccggtctc gcgtcagtgg catattccgt ctgccgatga cgatgcatgt
    23041 acgctaccgt acaagtcggg caattgcggc ttccggctcc gagccggggc ttccctcatc
    23101 aaaacgccca ccgcgcttat cgagccttgc aaatccacct gacggctgcc gcccacagat
    23161 cagacgcttg tggtcgaccg actgcaactc gttaggggtg tcttgcacgc gatcttctgt
    23221 cgcgccgcga acgcgggcgg cagcaggccg cgcattgcat gaacggaatt cttgtcagaa
    23281 cctgtatttc aattccaacc cgtattcccg cgtctcgttt ttcggttcga gtccgcgaac
    23341 accgaagccg atgggcacga tatccgaatc ctcgatgctc cagtaatgca tccaggggcc
    23401 gaatgcccac gagcccttc
//
LOCUS       DATBSZ010000143         5494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2275386, whole genome shotgun sequence.
ACCESSION   DATBSZ010000143 DATBSZ010000000
VERSION     DATBSZ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2275386"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VIG70_09700"
     CDS             <1..522
                     /locus_tag="VIG70_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY8250869.1"
                     /translation="AYLCDPKIRDNCRKNGDDPAALPLTYSDTIRKAIQGRPAGMTIT
                     MHTCRGNFKSAWVAEGGYEPVAEQMFSSGVDGFFMEFDSARAGGFEPLRFVPKGKKVV
                     LGLVSSKTGTLETGDDLKRKIDQAAKYVPVENLCLSPQCGFSSTHHGNKLSEDEQWRK
                     LERVVEVAREVWR"
     gene            519..890
                     /locus_tag="VIG70_09705"
     CDS             519..890
                     /locus_tag="VIG70_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8250870.1"
                     /translation="MSSDKAILELEDKRFSAMIARDLAALEKLTHGELLYTHSSGLTD
                     TKASWLESIKAGKAKYKSVDCSERKVRFLGEVALVNGKAAIEVDVDGKPRSLRLVFLN
                     AWTRTPQGWKFVAWQSCPLPA"
     gene            887..3160
                     /locus_tag="VIG70_09710"
     CDS             887..3160
                     /locus_tag="VIG70_09710"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8250871.1"
                     /translation="MKQFKGRREDDRLLTGRGRYTADWNLPGQLYGCFLRSDRAHAEI
                     VSLDTKAALGLPGVKAIFTGEDTADFKTPPPIVRYPGKGGAMIKVPHRDVMARKRVRY
                     VGQEVALVVATSAAAAQDAAEAIEVEYRELPVVVDARAALEAGAPQLHDEIPGNLAFD
                     YVYGDEQATEAAFARAAHVTRLELDSTRVFGTPMEPKACLAAYDAKSESFDVYTSTQG
                     LALMLPNLVAITGVPAEKIRIHAQDVGGGFGIRSQAYPEYCALMLASRKLGKPVKWVG
                     SRFETIVSDHHGRAASLEGELALDREGRFLGLRVRWVVNAGAFLSQPGPLINTLNPST
                     HAINVYRIPALYGRHRLALTNTTSTTAYRGAGRPNVSYLVERLVDEAARELDIDRAEI
                     RRRNFIPREAFPYKTPVGSTYDSGDPAGEFEEALEKSDWHDFESRRRKSASHGKLRGI
                     GCAVFCEPSGAAALPKEEAMIRFGSSGGATLHVLAGPSGQGHETVLPEVVGEILGLPA
                     EEITLKASDPADPPMVGAGTIGSRSMMSHGGALAVTAKEVVKKATDLAARALEVSPGD
                     VEFHAGKFQVKGTDLSISFREVARRYGPELDTRGSIPPPTAFPGGAHVAEVEIDRDTG
                     EVEVLRYVAVDDCGRVLNHVLLDGQLHGGIVQGLGQALTEHCVYDESSGQLLTGSFMD
                     YAMPRPEIMTRVELHDHSVPSPSNPLGVKGAGEAGTTGALPAVANAVIDALRPLGIHQ
                     LDFPFSPARVWGAMNKA"
     gene            complement(3157..3675)
                     /locus_tag="VIG70_09715"
     CDS             complement(3157..3675)
                     /locus_tag="VIG70_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8250872.1"
                     /translation="MKPDDVMFSATVRAEQARRGSRKGYAQAAAKGHFRRELPDEVVA
                     FIAERNSAYLATAGADGQPYVQHRGGPPGFLRVLDRSTVAFADYGGNRQYISTGNLAE
                     NERAFLFLMDYAEARRLKLWGRARVVADDSGLLARVADPGYGARIEQAIVFSVLAWDW
                     NCSQHIPRLVPA"
     gene            3751..4644
                     /locus_tag="VIG70_09720"
     CDS             3751..4644
                     /locus_tag="VIG70_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8250873.1"
                     /translation="MNKLAAMETFVRIVDKGTLTAAAEALDTSLPSVVRTLASLEREL
                     GVRLLNRTTRRIHLTDEGAHYLERCRAILSAVQETEAAFSAGRAEPQGRLAVTAPVVF
                     GRRYVAPIVGDFLRRHPKVTAELLFVDRVVNLVDEGIDVAVRIGRLKDSSLVAIPVGK
                     VRRVVCASPEYLRRAGTPRAPQEVRAHACVRTGLAPRGDWHFRVGRRTVAVPVNPVIS
                     CSDIDTGLDACVNGSGLGVFLSYQTASYRKEKKLSYVLEEFETEPVPVQVVYAQSRLV
                     TGKVRAFVDECVSRLRQARLD"
     gene            4652..5086
                     /locus_tag="VIG70_09725"
     CDS             4652..5086
                     /locus_tag="VIG70_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8250874.1"
                     /translation="MNLEVNGESRSVEAAADTPLLYVLRNELGLAGTRFGCGSGQCGA
                     CFVLIDGRAMATCDLPLSFATNKKITTIEGLGGTHPVQEALVRHQAAQCGYCMSGIVM
                     AAVALLSQNSNPTENQVRAALDRNLCRCGSHNRVVRAILDAR"
     gene            5076..>5494
                     /locus_tag="VIG70_09730"
     CDS             5076..>5494
                     /locus_tag="VIG70_09730"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY8250875.1"
                     /translation="MLAEKPAALSDARRLSQRVHFEGDGTVSIASGKVELGQGILTAL
                     AQIAAEELGVGLQRIRMLPASTAYSPDEGVTSGSQSIQEGGKALRAACAELRALMLER
                     AARHLGAPLAELKIEDGTIHGRGERVSYWDLADSEGE"
BASE COUNT          927 a         1829 c         1902 g          836 t
ORIGIN      
        1 gcctaccttt gcgacccgaa gatccgcgac aactgccgca agaacggcga cgacccggcc
       61 gcgttgccgc tgacgtattc cgacaccatc cggaaagcga tccagggccg gcctgcgggc
      121 atgacgatca cgatgcacac ctgccgcggc aacttcaaga gcgcgtgggt cgccgagggc
      181 ggctacgagc cggtcgcgga gcagatgttc tcgagcggcg tcgacggctt cttcatggag
      241 ttcgacagcg cgcgcgccgg cggtttcgag ccgctgcgtt tcgtgccgaa gggcaaaaaa
      301 gtcgtgctgg gcctggtgtc ttccaagacg ggaaccctgg aaaccgggga cgatttgaaa
      361 cgaaagatcg atcaggccgc caagtacgtt ccagtcgaaa atctctgcct gtcgccgcag
      421 tgcgggttct cgagcacgca ccacggcaac aagctcagcg aggacgagca gtggcgcaag
      481 ctcgagcggg tggtcgaagt ggcgagggag gtgtggcgat gagcagcgac aaggcaatcc
      541 tggagctgga agacaagcgc ttcagcgcga tgatcgcgcg cgacctggcg gcgctcgaga
      601 agctgacgca cggcgagctt ctctataccc attcttcggg cctgaccgac acgaaggcga
      661 gctggctaga gtcgatcaag gccggcaagg cgaagtacaa gagcgtcgac tgcagcgagc
      721 gcaaggtccg cttcctgggc gaagtggcgc tggtgaacgg caaggcggcg atcgaagtcg
      781 acgtcgacgg caagccgagg tcgctcaggc tcgtcttcct caacgcctgg acgcgcacgc
      841 cgcaaggctg gaagttcgtc gcctggcagt cctgtccgct gccggcgtga agcaattcaa
      901 gggtcgccgc gaggacgacc ggcttctcac cggccgcggc cggtacaccg ccgactggaa
      961 ccttcccggc cagctctacg gctgctttct gcgctcggat cgagctcacg cggaaatcgt
     1021 gtccctggat accaaggccg ccttgggtct tccgggagtg aaagccatat tcacgggcga
     1081 agacactgcc gatttcaaga cgccgccgcc gatcgtgagg tacccgggaa agggcggcgc
     1141 gatgatcaag gtgccgcacc gggacgtgat ggcgcgaaag cgggtgcgct acgtcgggca
     1201 ggaagtggcg ctggtggtcg cgacttcggc cgcggcggcg caggacgctg cggaggcgat
     1261 cgaggtcgag taccgcgagc tgccggtcgt cgtggacgcc cgcgccgcgc tggaagccgg
     1321 cgcgccgcag ctccacgacg agattccggg caatctcgcc ttcgactacg tgtacggcga
     1381 cgagcaggcg accgaggcgg cgttcgcccg cgccgcgcac gtcacgcgcc tcgagctcga
     1441 ctcgactcgc gtcttcggca caccgatgga gccgaaggcc tgtctcgccg cctatgacgc
     1501 gaagagcgag agctttgatg tctacaccag cacccagggg ctcgcgctga tgctgcccaa
     1561 cctggtggcg atcaccggcg tgccggccga gaagatccgc atccacgccc aggacgtcgg
     1621 cggcggcttc ggcatccgct cgcaggccta ccccgagtac tgcgcgctga tgctcgcctc
     1681 ccgcaagctc gggaagccgg tgaagtgggt cggctcgcgc ttcgaaacca tcgtcagcga
     1741 ccaccacggc cgcgccgcca gcctcgaggg cgagctcgcg ctcgatcgcg aggggcgctt
     1801 cctcggtctg cgcgtgcggt gggtggtgaa cgccggcgcg ttcctttcgc agcccgggcc
     1861 gctcatcaat acgctgaacc cgtcgacgca cgcgatcaac gtctaccgca tcccggcgct
     1921 ctacggccgg caccggctcg cgctcaccaa caccacctcg accaccgcgt accgcggcgc
     1981 cggccggccg aacgtgtcct acctggtcga gcggctggtc gacgaggcgg cgcgcgagct
     2041 cgacatcgac cgtgccgaga tccgccggcg caatttcatt ccgcgcgagg ccttccccta
     2101 caagacgcca gtgggctcca cctacgacag cggcgatccc gcgggcgaat tcgaagaagc
     2161 tctcgagaaa tcagactggc acgacttcga atcgcggcgc cgaaagtctg cctcccatgg
     2221 aaaacttcgt ggcatcggct gcgcggtgtt ctgcgagccc tcgggcgcgg ccgcgctgcc
     2281 gaaggaagag gcgatgatcc ggttcggttc ctcgggcggt gcgacgctgc acgtgctcgc
     2341 cgggccgtcg ggacaggggc acgaaacggt gctgcccgaa gtcgtggggg agatcctcgg
     2401 cctgccggcg gaagaaatca cgttgaaggc gagcgacccg gccgatcccc cgatggtcgg
     2461 cgccggcacg atcggctcgc gcagcatgat gtcgcacggc ggcgcgctcg cggtgacggc
     2521 gaaggaagtc gtgaagaagg cgaccgacct cgcggccagg gcgctcgagg tttcccccgg
     2581 cgacgtcgag ttccatgccg gcaaattcca ggtgaaaggc accgaccttt cgatcagctt
     2641 cagggaggtc gcgcgccgct acggtcccga gctcgatacg cgcggtagca ttcccccgcc
     2701 gacggcgttt cccggcggcg cgcacgtggc cgaggtcgag atcgaccgcg acaccggcga
     2761 agtcgaggtg ctgcgctacg tcgcggtcga cgattgcggc cgggtgctga accacgtgct
     2821 gctcgacggc cagcttcacg gcggcatcgt gcagggcctg gggcaggcgc tcaccgagca
     2881 ttgcgtgtac gacgagtcga gcggacagct cctcaccggc tcgttcatgg actacgcgat
     2941 gccgcggccg gaaatcatga cccgcgtcga gctgcacgac cattcggtcc cctcgccgag
     3001 caacccgctg ggggtcaagg gtgcggggga agccggcacg accggcgcgt tgcccgcagt
     3061 ggcgaacgcg gtgatcgacg cgctacgtcc gctcggcatc caccagctcg actttccgtt
     3121 ctcgcccgcc cgtgtttggg gggcaatgaa caaagcctag gcaggcacca gccgcgggat
     3181 gtgctgcgag cagttccagt cccaggcgag cacgctgaaa acgatcgcct gctcgatgcg
     3241 cgcgccgtag cccggatcgg ccacgcgcgc gagcaggccg gagtcatccg cgaccacgcg
     3301 tgcgcggccc cagagcttga gccggcgcgc ctccgcgtag tccatcagaa acaggaacgc
     3361 gcgctcgttc tcggccaggt tgcccgtgct gatgtactgc cggttgccgc cgtaatcggc
     3421 gaacgcgacg gtactgcgat cgaggacgcg caggaacccg ggcgggccgc cgcgatgctg
     3481 cacgtagggc tgcccgtccg cgcccgcggt cgcgagatag gccgagttgc gctcggcgat
     3541 gaaggcgacc acctcgtccg gcagctcgcg ccggaagtgg cccttggcgg cggcctgcgc
     3601 atagcccttg cgggatccgc ggcgcgcctg ctcggcgcgc acggtggcgg agaacatcac
     3661 gtcgtcgggt ttcatgcggg gtaggttccc acttcttcgt tccggaataa atagggtatc
     3721 ctgcagtgga ctactccgga atctgaagta atgaacaagc tcgccgccat ggagaccttc
     3781 gtccgcatcg tggacaaggg gaccctcacc gccgctgccg aggcgctcga cacgtcgctc
     3841 ccatccgtgg tgcgcacgct cgcctcgctc gagcgcgagc tcggcgtgcg cctgctgaac
     3901 cgcaccacga ggcggatcca cctgaccgac gaaggcgcgc actacctgga gcgctgccgg
     3961 gcgatcctct cggcggtgca ggaaaccgag gcggcgttca gcgccggacg cgccgagccc
     4021 cagggcaggc tcgcggtcac ggcgccggtc gtattcggcc ggcggtacgt cgcgcctatc
     4081 gtgggcgatt tcctgcggcg gcaccccaaa gtgaccgcgg agctcctgtt cgtcgaccgg
     4141 gtagtgaacc tggtcgacga aggaatcgac gtcgcagtgc gcatcgggcg tctgaaagat
     4201 tcgtcgctcg tcgcgattcc agtggggaag gtccggcggg tcgtctgcgc gagccccgag
     4261 tacctgaggc gcgcgggcac gccgcgcgcg cctcaggagg tccgcgcgca tgcctgcgtc
     4321 cggaccgggc tcgcgccgcg cggcgactgg cactttcgcg tcggacgacg aaccgttgcc
     4381 gtgccggtaa accccgtaat atcgtgcagc gatatcgaca ccggcctgga tgcgtgcgtg
     4441 aacggcagcg gcctcggcgt gttcctgtcc taccagacgg cgtcctaccg gaaggaaaag
     4501 aagctttcct atgtgcttga ggaattcgaa accgaacccg tgcccgtgca ggtggtgtac
     4561 gcccagtccc ggctggtcac cgggaaagtg cgcgctttcg tggacgagtg cgtgagccgg
     4621 ctgcgccagg cgcggctgga ctgatccggc aatgaacctc gaggttaacg gcgagagccg
     4681 cagcgtcgag gcggcagcgg acacgccgct tctctacgtc ctgcgcaacg agctcggcct
     4741 cgccggcacc cgcttcggtt gcggcagcgg gcaatgcggc gcgtgcttcg tgctcatcga
     4801 cggccgggca atggctacgt gcgacttgcc gctgtctttt gcgacgaata agaaaatcac
     4861 cacgatcgag ggcctcgggg gcacgcaccc tgtccaggag gcgctcgtcc ggcaccaggc
     4921 ggcgcagtgc ggctactgca tgagcggcat cgtcatggct gcggtcgcgt tgctttctca
     4981 gaattccaac cccacggaaa atcaggtgcg tgccgccctg gaccgcaatc tctgccgctg
     5041 cggctctcac aaccgcgtcg tgcgcgcgat cctcgatgct cgctgaaaag cccgcggcgc
     5101 tatcggacgc gaggcgcctt tcgcagcgcg tgcatttcga gggcgacggc acggtgtcga
     5161 tcgcgagcgg caaggtggag ctcggccagg gaatcctgac cgcgctggcg cagatcgcgg
     5221 ccgaggagct cggcgtcggc ctgcagcgaa tccgcatgct gccggcgagc accgcctaca
     5281 gccccgacga gggcgtgacc tcgggcagcc agtcgatcca ggaaggcggc aaggcgctgc
     5341 gcgctgcgtg cgccgagctg cgcgccctga tgctcgagcg ggcggcgcgc catctcggcg
     5401 cgccgctggc cgagctgaag atcgaagacg gcacgatcca cggccgcggc gagcgggtca
     5461 gctactggga tcttgccgat tccgagggcg agat
//
LOCUS       DATBSZ010000144         5724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2207597, whole genome shotgun sequence.
ACCESSION   DATBSZ010000144 DATBSZ010000000
VERSION     DATBSZ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2207597"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..323)
                     /locus_tag="VIG70_09735"
     CDS             complement(120..323)
                     /locus_tag="VIG70_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY8250876.1"
                     /translation="MATGTVKWFNDSKGFGFITPSEGGEDLFAHHTAIQMSGFKTLKE
                     GQKVEFEVKRGPKGLQAHNIKPL"
     gene            575..3208
                     /gene="mdeB"
                     /locus_tag="VIG70_09740"
     CDS             575..3208
                     /gene="mdeB"
                     /locus_tag="VIG70_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate dehydrogenase"
                     /protein_id="HEY8250877.1"
                     /translation="MTDLADIVSSAFWRVNPPDADPAETREWLDAFDALVETEGRERA
                     TFLLRKLLDHARARRVPLPPVLNTPYKNSIALAHQPQFPGNLELESRISAIVRWNALA
                     MVVRANRAHPELGGHIASYASAADLFEVGFNHFFRIDDLVYFQPHSAPGVYARAFLEG
                     RLTEDQLNHYRQETGGNGLSSYCHPYLMPEFWQFPTGSMGLGPINAVYQARFMRYLEN
                     RGLAEKASPETNSRKVWCFIGDGEMDEPESLAGLSLATREGLDNLIFVVNCNLQRLDG
                     PVRGNGSVVQELEGLFAGAGWNVVKLLWGSDWDALFARDEDGVLLKRLHETVDGEFQT
                     YAATDGRFNREHFFNKYPELRELIAGMSDDDIDRLRRGGHDPVKIYAAYHAAAHYKGQ
                     PTVILAKTKKGYGMGNWGQGKMGTHQQKKVDDEALREFRDRFALPLSDEDVAQLRFYH
                     PGPASPELKYLTDRRQGLGGYLPRRITKAESIAVPSLSASERSQSTTMVFVQLLSQLL
                     KDEKLGRRVVPIVADEARTFGMQTLFRQVGIYSAVGQLYEPEDHDELLYYREAKDGQI
                     LEEGITEAGALSSWLAAATSYSSHAEPMLPFYIYYSIFGFQRVGDLVWAAADSRARGF
                     LVGGTAGRTTLAGEGLQHQDGSSHLAAATIPNCRAYDPCFGYELAAIVEDGARRMVEA
                     QEDVFYYLTVANENYPHPAMPEGARQGILKGMYLYKKNEKAKLQLLGSGPILREVLAA
                     AELLEKDWKVAANVWSVTSFTELRRDGMKAERVRRLGGKATSWVEECLQKAEGPIIAA
                     SDYVRAVPDLIRPYLSKPYVSLGTDGFGRSDTRAALRAFFEVDARHIAVAALAAIDPG
                     LLREALLRYGIDQALLPPWER"
     gene            complement(3143..3535)
                     /locus_tag="VIG70_09745"
     CDS             complement(3143..3535)
                     /locus_tag="VIG70_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250878.1"
                     /translation="MRILVAWALCLAASASLANDQEIQRALIQRDQQSAEFAARVRGA
                     DTSALQQLHSRQLLEATTQPPHPDAAIARQLQPYQRQKMADERVLVLTPPTVSSAPRP
                     EKPAPRLLPLPGGQERLIDPIPQQGLTQ"
     gene            complement(3532..4497)
                     /locus_tag="VIG70_09750"
     CDS             complement(3532..4497)
                     /locus_tag="VIG70_09750"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEY8250879.1"
                     /translation="MGTVVALGGQVGGAKLADGLYRLRGRDLALIVNTGDDYEHLGLA
                     FSPDLDTALYALAGIAGAAAGWEPEAESHALHGMLKALGGPEHPRVGDKSLAAPLLRT
                     QGLASGRRLTQITLDFCRHLGIGARVLPMSDDPVRTHVLTDGGAIPFPEYFGQLGCEP
                     RVRGLEYAGADQARIPDEVLDALHAADLEAVVICPSNPYHSIGPILEISGMRELLRKR
                     GAPVIAVTPIVGGKALKGSAGKMMRELGKEPSPRAVAIQYLRLIDGFVIDREDTHLVE
                     SVRSLGIHVLAAPTVMRAPDDRVALARTVLEFARSVREAKALEVE"
     gene            complement(4516..5175)
                     /locus_tag="VIG70_09755"
     CDS             complement(4516..5175)
                     /locus_tag="VIG70_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8250880.1"
                     /translation="MSIALIGGAGEGLGAALGRRFSKAHEVVLSSRTQGERADLREEA
                     QVIELFDRIEKRGPVEVAIFNAGANFRASILETPAEMFEKVWRLGCYAGFLFGREAAR
                     RMMPRGRGTILFTGATASVRGSAQFAAFAAAKGGLRQVAQSMARELGPKNIHVAHVVI
                     DGMIDNRRTRERMADRVKDLPADGMLATDAIAELFWQLHAQPRSAWTFEADLRPWAER
                     F"
     gene            complement(5172..>5724)
                     /locus_tag="VIG70_09760"
     CDS             complement(5172..>5724)
                     /locus_tag="VIG70_09760"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8250881.1"
                     /translation="PPIPGIDSPNVVSYVDVLRDAVKPGRNVAIIGMGGIGFDVALYL
                     LERGSRAPLDPEAFAAHWGISGEPPHAAPRHSITMLKRSHTPFGHTLGRTTGWVHRAE
                     LARNGVRMIKGVEYRRIDHAGVHIAADGKEMLVAADTVIVCAGQDPRKEVKGTHVIGG
                     AKEAAELDAKRAMLEGAELAAQL"
BASE COUNT          936 a         1912 c         1920 g          956 t
ORIGIN      
        1 tcgtggtcac caatgccccg ggctcgctcg gcaactgcat gccggagaag ccttccgggg
       61 gttgcccctg aaatagaaaa gcccgcttgc gcgggctttt ttttccttgt gccgggtgct
      121 tacagcggct tgatgttgtg cgcctgcaga cccttcgggc cgcgcttcac ctcgaactcg
      181 accttctgtc cttccttgag ggtcttgaag ccggacatct ggatcgcggt gtgatgcgcg
      241 aacaggtcct cgccgccctc gctcggggtg atgaagccga agcctttgga atcgttgaac
      301 cactttacgg taccggttgc cattactgtt tacctctacg aaaagccgcg cgaatccggg
      361 cgcgacggag ccgggcacga agaatcaaga gtgggaatgc actgccacta cgagcgctgc
      421 acttgaggcg gcgatgaagg gtcggcaatt tcgctgcttg aaaagcatcc tgcggcgcaa
      481 ataatagcat cgttttccgg cgcccgggca tggcccggaa ggcggcgcca cggctggtat
      541 ccaatttgct tggcgcgcgg attaccatgt ccggatgacc gacctcgccg acatcgtttc
      601 ctccgcgttc tggcgggtaa atccgcccga cgccgacccc gccgaaaccc gcgagtggct
      661 cgacgccttc gacgcgctcg tcgagaccga agggcgcgag cgcgcaacgt tcctgctgcg
      721 caagctcctc gatcacgcgc gcgcgcggcg cgtgccgctg ccgccggtgc tgaacacgcc
      781 ctacaagaac tcgatcgcgc tcgcccacca gccacagttc ccgggcaacc tcgagctcga
      841 aagccgcatt tccgcgatcg tgcgctggaa cgccctcgcc atggtggtac gcgcgaaccg
      901 cgcgcatccc gagctcggcg gtcacatcgc gagctacgcg tcggcggcgg atctgttcga
      961 ggtcggcttc aaccactttt ttcgcatcga cgacctggtg tacttccagc cgcattccgc
     1021 gcccggggtt tacgccaggg cgtttctcga agggcgactc acggaagatc agctcaatca
     1081 ctaccggcag gaaaccgggg gaaacggtct ctcctcgtat tgccatccgt acctgatgcc
     1141 ggagttctgg cagttcccca ccggctcgat gggcctcgga ccgatcaacg ccgtctacca
     1201 ggcgcgcttc atgcggtatc tcgagaaccg cggtttggca gaaaaagcct cacccgagac
     1261 gaattcccgc aaggtctggt gcttcatcgg cgacggcgag atggacgagc cggaatcgct
     1321 cgcggggctg tcgctcgcca cacgcgaggg cctggacaac ctgatcttcg tcgtcaactg
     1381 caacctgcag cggctcgacg gcccggtgcg cggcaacggc tcggtcgtgc aggagctcga
     1441 aggactgttc gccggcgccg gctggaacgt ggtgaagctc ttgtggggat cggactggga
     1501 tgccctcttc gcgcgcgacg aggacggcgt gctgctcaag cggctgcacg agaccgtcga
     1561 cggcgagttc cagacctacg cggcgaccga cggccgcttc aatcgcgagc acttcttcaa
     1621 taagtacccg gagctgcgcg agctcatcgc cggcatgagc gacgacgaca tcgaccgcct
     1681 gcgccgcggc ggccacgacc cggtcaagat ctacgccgcg taccacgccg ccgcacacta
     1741 caagggacag cccacggtga tcctcgccaa gacaaagaag ggctacggca tgggcaactg
     1801 gggccagggc aagatgggca cccaccagca gaagaaggtc gacgacgagg cgctgcgcga
     1861 attccgggac cgcttcgcgc tgccgctttc cgacgaagac gtggcgcagc tgcgtttcta
     1921 tcacccagga cccgcgagcc ctgagctcaa atacttgacg gatagaaggc agggcctggg
     1981 cggctatctt ccgcgccgaa tcaccaaggc ggaatcgatc gcggtgccgt cgctgagcgc
     2041 atcggagcga agccagtcga ccacgatggt gttcgtgcag ctcttgtcgc agctcctcaa
     2101 ggacgagaag ctgggacggc gcgtggtgcc gatcgtcgcc gacgaggcgc gcactttcgg
     2161 catgcagacg ctgttccgcc aggtcggcat ctactcggcg gtgggccagc tctacgagcc
     2221 cgaggaccac gacgagctgc tctactaccg cgaagcgaag gacgggcaga tcctggagga
     2281 gggcatcacc gaggccggcg cgctctcctc ctggcttgcc gcggcgacct cgtattcgtc
     2341 gcacgccgag ccgatgctgc cgttctacat ctactactcc atcttcggct tccagcgcgt
     2401 cggtgacctc gtctgggccg cggccgattc gcgcgcgcgg ggctttctgg tcggcggcac
     2461 cgcgggacgc actacgctcg cgggcgaggg cctgcagcac caggacggct cctcgcatct
     2521 cgcggcggcg acgatcccga actgccgcgc ctacgatccg tgcttcggct acgagctcgc
     2581 cgcgatcgtg gaggacggcg cgcggcgcat ggtggaagcg caggaagacg tgttctatta
     2641 cctcacggtg gccaacgaga actacccgca tccggcgatg ccggaggggg cgcgccaggg
     2701 aatcctcaag gggatgtacc tgtacaagaa aaatgaaaag gcgaagctcc agctgctggg
     2761 cagcgggccg atcctgcgcg aagtgctcgc ggccgccgag ctgttggaga aggactggaa
     2821 ggtggcggcc aatgtctgga gcgtgacgtc gtttaccgag ctgcgccggg acggcatgaa
     2881 ggcggagcgc gtgcgcaggc tcggcggaaa ggcgacgagc tgggtcgagg agtgccttca
     2941 gaaagccgag ggaccgatca tcgcggcgag cgactacgtg cgcgctgtcc cggacctgat
     3001 ccggccttat ctttcgaagc cgtacgtgag cctcggcacc gacggcttcg gccgcagcga
     3061 tacgcgcgcc gcgctgcggg cgttcttcga agtcgatgca cgccacatcg ccgtcgccgc
     3121 gctcgcggcg atcgaccccg gcctactgcg tgaggccttg ttgcggtatg ggatcgatca
     3181 ggcgctcctg cccccctggg agcggtagca gccggggggc aggcttttcg gggcgcggcg
     3241 ccgagctcac cgtcggcggc gtgagcacga gcacgcgctc gtccgccatc ttctgccttt
     3301 gatagggctg gagctggcgt gcgatcgcgg catccggatg gggcggctgg gtcgtagctt
     3361 cgagcagctg ccgcgagtgt agctgttgca gcgccgaggt gtcggcgccg cgcacgcgcg
     3421 cggcgaattc cgcggattgc tggtcgcgct ggatgagcgc gcgctggatc tcctggtcgt
     3481 tcgccaggga tgcggacgcc gcgaggcata acgcccaggc aacgaggatc ctcattccac
     3541 ctccagggcc ttggcctcgc gcacgctgcg cgcgaactcg agcacggtgc gcgcgagcgc
     3601 cacgcgatcg tcgggcgcgc gcatgacggt cggcgcggcg agcacgtgaa tcccgagcga
     3661 acgcacgctt tcgacgaggt gcgtgtcctc gcggtcgatg acgaagccgt cgatgaggcg
     3721 caggtactgg atcgcgaccg cgcgcggcga aggctccttg cccagctcgc gcatcatctt
     3781 gcccgccgat cccttgagcg ccttgccccc gacgatcggg gtgaccgcga tcaccggcgc
     3841 gccgcgcttg cgcagcagct cgcgcatgcc gctgatctcg aggatcgggc cgatcgagtg
     3901 atacgggttg gaagggcaga tcaccacggc ttcgaggtcg gcggcgtgca gcgcgtcgag
     3961 cacttcgtcg gggatgcgcg cctggtcggc acccgcgtac tcgagcccgc gcacgcgcgg
     4021 ctcgcagccg agctggccga aatattccgg gaacggaatc gcgccaccgt cggtgagcac
     4081 gtgcgtgcgc accggatcgt cgctcatcgg cagcacccgc gccccgatgc cgaggtggcg
     4141 gcagaagtcg agcgtaattt gggtcaggcg ccgccccgaa gcgagtccct gcgtgcgcag
     4201 cagcggcgcc gcgagcgact tgtcgccgac ccgcgggtgc tccggcccgc cgagcgcctt
     4261 cagcatgccg tgcagcgcgt ggctttcggc ttcgggctcc cagcccgcgg ctgcgccggc
     4321 aataccggcg agcgcataga gcgcggtgtc gaggtcgggc gagaacgcga gcccgaggtg
     4381 ctcgtagtcg tcgcctgtgt tcacgatcaa cgcgaggtcg cgcccgcgca ggcgatacag
     4441 gccgtcggcg agcttcgcgc cgccgacctg tcctccaagt gctacgaccg tgcccatgcg
     4501 aggattctaa tgaaactaga acctctccgc ccagggacgc aggtcggcct cgaaggtcca
     4561 cgccgagcgc ggctgcgcat ggagctgcca gaacagctcg gcgatcgcgt cggtggcgag
     4621 catgccgtcg gcgggcaggt ccttcactcg gtcggccatg cgctcgcgcg tcctgcggtt
     4681 gtcgatcatg ccgtcgatca ccacatgcgc gacgtggatg ttcttcggtc cgagctcgcg
     4741 cgccatggac tgtgccacct ggcgtagccc gcccttcgcg gcggcgaagg cggcgaactg
     4801 cgccgagccc cgcaccgaag cggtcgcgcc ggtgaacagg atcgtgcctc tgccgcgcgg
     4861 catcatacgc cgcgccgctt cccgcccgaa caggaagccg gcatagcacc cgagccgcca
     4921 gactttctcg aacatctcgg ccggggtctc gaggatcgag gccctgaagt tcgcgccggc
     4981 gttgaagatc gcgacctcga ccgggccgcg cttctcgatg cggtcgaaga gctcgatcac
     5041 ctgcgcttct tcgcgcaggt cggcgcgttc gccctgtgtt ctcgacgaca acaccacctc
     5101 gtgggctttt gaaaacctcc ggccgagcgc cgcgccaagg ccttcgccgg cgccgccgat
     5161 gagcgcgatg ctcatagctg cgccgccagc tctgcgcctt cgagcatcgc ccgcttggca
     5221 tcgagctccg cggcttcctt ggcgcccccg atcacatggg tccctttgac ctcttttcgc
     5281 gggtcctggc cggcacagac gatcacggta tctgcagcca cgagcatctc ctttccatcg
     5341 gcggcgatat gcacgccggc gtggtcgatc ctgcggtact cgacgccctt gatcatgcgc
     5401 acgccgttgc gcgcaagctc ggcgcgatgc acccacccgg tggtgcggcc gagcgtgtgg
     5461 ccgaacggcg tgtgcgagcg cttgagcatg gtgatgctgt ggcggggggc agcatgaggg
     5521 ggttctcctg aaatacccca gtgcgcagcg aaagcttcag gatccagcgg agcgcggctt
     5581 cctcgttcga ggaggtagag cgcgacgtcg aagccgatgc cgcccatgcc gatgatcgcg
     5641 acattgcggc cgggcttcac tgcgtcgcgc agcacgtcga cataactcac cacgttcggg
     5701 ctatcgatgc cggggatcgg cggc
//
LOCUS       DATBSZ010000145         8763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1114534, whole genome shotgun sequence.
ACCESSION   DATBSZ010000145 DATBSZ010000000
VERSION     DATBSZ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114534"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1606
                     /locus_tag="VIG70_09765"
     CDS             32..1606
                     /locus_tag="VIG70_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8250882.1"
                     /translation="MKFRNHLIAVAACAAIGAHAQTVRIANQGDSLSMDPHSLNESLQ
                     LSVTGNVYEPLVGRGKDLSLAPALATSWKATSPTVWRFELRKNVTFHDGTPFTADDVV
                     FSFERAPNVEGSPSSFGIYARGKTFTKVDDHTVHIKTAAPYPLMPNDVSQVFIVSRKH
                     GQGAKTPDYNSGKAAIGTGPYKFVEYTPGNRIVMQRNDQYWGDKPAWARVVFRGIKSD
                     PSRVAALLAGDVDLIDEVPATDMARLKKDPKVTIAQTVSNRIIYLHLDHFRDDSPFVL
                     GKDGKPIKSPLRDVRVRRAISMAIDRDAIVSRVMEGQAIKAGQLLPEGFFGVSSKLKP
                     VGFDPNGAKKLLAEAGVPNGFRMTIHSPNDRYPNDAKIAEAVGQMLSRIGIDTQVVTM
                     TQGVFFRDASTGSPDKGPKFSFILVGWGSGTGEASSPLKSLLATFDRDKGMGASNRGR
                     YSNPQVDKLINDALATNEDAKRAELLARATELAIEEVGIIPLHYQVNTWALRRGFGYK
                     ARTDEYTLATEVSKEK"
     gene            1603..2550
                     /locus_tag="VIG70_09770"
     CDS             1603..2550
                     /locus_tag="VIG70_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8250883.1"
                     /translation="MMVFIIRRVLQSLVVVLVMSLVVFVGVHLVGDPVHILISDDMTQ
                     IEIERFIQKLGLDRPVHEQYFHFLANAARGDLGNSFVFGEPALKLILQRMPATLELAF
                     AAFLLAIVLGIPLGMYAGLRPESPASKAIMAGSILGFSLPTFWVGLMLIMVFAVMLGW
                     LPSTGRGDFMTWDGLRHLALPAINLALFKMALVTRLARAGTREAALQDYVKFARAKGL
                     SPARVVLVHVLKNILVPVVTVLGLELGGLIAFSVVTETVFAWPGMGKLLIDSIQRLDR
                     PVIVAYLVITVLMFVVINLIVDILYSMLDPRVRLKEAKA"
     gene            2547..3455
                     /locus_tag="VIG70_09775"
     CDS             2547..3455
                     /locus_tag="VIG70_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8250884.1"
                     /translation="MSAAVETLSIAKPETPFRRLAREFFASPVATGSFAALLIIVFLA
                     LFAPLLAPQNPYDLAKVTVMDARLAPGETGSAGFMMWLGADGAGRDMWSAILYGLRIS
                     LGVGVASGVVALGIGTALGLAAAYRGGWLDALIMRLVDLQLSFPAILVALILVAILGR
                     GADKVMIALILVQWAYYARTVRGSALVERRKEYIEAAQCLALPPRRIVFRHLLPNVIA
                     PMIVVGTLQTAHAISLEATLSFLGVGLPLTEPSLGLLIANGFEYMLSGKYWISFYPGI
                     ALLATIMSINLVGDRLRDVLNPRLER"
     gene            3445..4410
                     /locus_tag="VIG70_09780"
     CDS             3445..4410
                     /locus_tag="VIG70_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8250885.1"
                     /translation="MNGNTLEVQDLKTHFFTRAGVVRAVDGVSFEVRPGEVLGLVGES
                     GCGKTVTGFSILGLVDAPGRIIGGRILFDGTDLLKLGEHELRRIRGARIAMVFQDPMM
                     TLNPVLRIDTQMVEAVRAHQDVDSRMALKIAREALERVGIPSPESRLRSYPHQFSGGM
                     RQRVAIAIALLNRPKLIIADEPTTALDVTIQAQILYEAQKLCRESGTALVWITHDLSV
                     VAGLADRICVMYAGRIVEQGSVDEVLDTPLHPYTHGLIGSVPSRNRRGSPLSQIPGMM
                     PALANLPPGCAFRNRCPRAASACLEEPKLSSHKNRLVRCFYPMHA"
     gene            4407..5387
                     /locus_tag="VIG70_09785"
     CDS             4407..5387
                     /locus_tag="VIG70_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8250886.1"
                     /translation="MSEPLVELQKVSKRFVQPLDFAGKIARALGADVADEVVHAVEDV
                     SFAVHEGEVVGLVGESGCGKSTLGRMVAGILEPSAGRIRYRGRDGAGRQRAGLQVQMI
                     FQDPFASLNPRMRVADIVGEAPLVHGIVRREELDGYLDALLQRCGLDPSYKPRYPHQF
                     SGGQRSRIGIARALAVKPEFLVCDEAVAALDVSIQAQILNLFMALRRDLGLTYLFISH
                     DLGVVEHLSDRVVIMYLGRVVESASTDGIFARANHPYTQALLKQVPRLDTRKTQFKPI
                     IGEIPSPLAPPTGCHFHPRCPHAMPRCKDEAPPLKEIGSGHLSACHLNDR"
     gene            complement(5384..5722)
                     /locus_tag="VIG70_09790"
     CDS             complement(5384..5722)
                     /locus_tag="VIG70_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250887.1"
                     /translation="MSSLCFTGDGLTASLFAAALHFGWTPADGVAREESWHVRQGHFE
                     FFAARIRKQGGNDDDWEHYWPMRAPQARLVFDRRGLREDYVICGEMWCRPLEHFLPRS
                     RAPVVQLAPC"
     gene            complement(5715..7199)
                     /locus_tag="VIG70_09795"
     CDS             complement(5715..7199)
                     /locus_tag="VIG70_09795"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEY8250888.1"
                     /translation="MKSQQVTGPRVAVLGLHLESNAFAAVSEERHFRSLCYCAGEEIS
                     REARKKVSALPAEIPGFYGEMDALGPWTPVPIVVTACEPGGAVDERFFQATLAAMRAR
                     LQAAGKLDAVYISNHGGMISTGGPDPDGELYAMVRGAAGPRTPVVATVDLHANISERM
                     VANVDALVSYRTNPHMDQAERAVDAARLLTGFIGGKKSKRAFVRLPLTPASVTLLTRE
                     GPYADAIRYGQTRVGNGVLDVSIVGGFVFSDTPKNGVAIVINGERGADVRGTALDIAG
                     RIWADRKRFVKRLTSMEDALRISGKTIWSDAGDNPGGGGTGQTTTLLQRLIEREVREV
                     LYGVFFEPQIAAAAKCKREIEAVFTEGFRAPATVLKTTDGQCIGRRGIWAGRALDLGP
                     TCALQIGGVTVVCVSKRKQCAAPVFFEMHGLDIRAARTVVVKSRGHFRGGFDELFPPD
                     RVIEVDTPGLTSPVLERLEFKGLPRPVFPLDADAQWTPGAAVYE"
     gene            7224..7469
                     /locus_tag="VIG70_09800"
     CDS             7224..7469
                     /locus_tag="VIG70_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250889.1"
                     /translation="MKRSIALMFIPALLLAGCSRPVVKETVVERPVVVERAAATGASA
                     PSCTYASQAYSHGSVSCQDRTQFRCDNGIWNRTFTAC"
     gene            complement(7500..7694)
                     /gene="iscX"
                     /locus_tag="VIG70_09805"
     CDS             complement(7500..7694)
                     /gene="iscX"
                     /locus_tag="VIG70_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEY8250890.1"
                     /translation="MKWTDTQDIAIALSESHPDKDPTSVNFVDLYKWVLALPGFSDDP
                     KRCGERILEAIQQAWIEEAE"
     gene            complement(7691..8041)
                     /gene="fdx"
                     /locus_tag="VIG70_09810"
     CDS             complement(7691..8041)
                     /gene="fdx"
                     /locus_tag="VIG70_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEY8250891.1"
                     /translation="MAKIKVLPGHALCPQGAEIEARSGVSICDTLLERHIDIEHACEK
                     SCACTTCHVIVRQGYDSLEPPEEKEEDLLDKAWGLEATSRLSCQAKVADQDLVIEIPK
                     YTINYEREGGGAKK"
     gene            complement(8041..>8763)
                     /locus_tag="VIG70_09815"
     CDS             complement(8041..>8763)
                     /locus_tag="VIG70_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY8250892.1"
                     /translation="AGNKPQGEDWLLLDVIPLSLGIETMGGLTEKIIPRNTTIPAGRA
                     QEFTTFKDGQTGMSIHVVQGERELVSDCRSLARFELKGIPPMVAGAARIRVAFQVDAD
                     GLLSVTAKEGTSGVEASVTVKPSYGLGDADIERMLRDSFEHAREDTHARALAEQRVDG
                     QRLIEATRSALVADAKLLNAEERAAIDAAIASLEKLLPGTEHRAIRQAVEALNRATDE
                     FAARRMDEGIKRALAGRRIGSL"
BASE COUNT         1377 a         3070 c         2825 g         1491 t
ORIGIN      
        1 ctgatttttc ctgcaaagaa ggagtcaccg aatgaagttc cggaaccacc tcatcgccgt
       61 tgcagcctgc gccgccatcg gcgcgcacgc gcagaccgtg cgcatcgcca accagggcga
      121 ctcgctgtcg atggacccgc actccctgaa cgagtcgctg cagctgtccg tcaccggcaa
      181 cgtgtacgag ccgctggtcg gccgcggcaa ggacctgtcg ctggcgccgg cgctggctac
      241 ctcatggaag gcgacgtcgc cgacggtctg gcgcttcgag ctgcgcaaga acgtcacgtt
      301 ccacgacggc acgccgttca ccgccgacga cgtggtcttc agcttcgagc gcgcgccgaa
      361 cgtggagggc agcccctcga gcttcggcat ctacgcgcgc ggcaagacct tcaccaaggt
      421 cgacgaccac acggtgcaca tcaagaccgc ggcgccctac cccctcatgc cgaacgacgt
      481 ctcgcaggtg ttcatcgtgt cgcgcaagca tggccagggc gcgaagacgc cggactacaa
      541 ctcgggcaag gccgcgatcg gcaccgggcc gtacaagttc gtcgagtaca cgcccgggaa
      601 ccgcatcgtc atgcagcgca acgaccagta ctggggcgac aagccggcct gggcgcgcgt
      661 ggtgttccgc ggcatcaagt ccgacccgtc gcgcgtcgcg gcgctgctcg cgggcgacgt
      721 cgatctgatc gacgaagtgc cggcgaccga catggcgcgg ctcaagaagg atccgaaggt
      781 cacgatcgcg cagacggtct ccaaccgcat catctatctc cacctcgacc atttccgcga
      841 cgactcgccg ttcgtgctcg gaaaggacgg caagccgatc aagagcccgc tgcgcgacgt
      901 gcgtgtgcgc agggcgatct cgatggcgat cgaccgcgac gcgatcgtga gccgcgtaat
      961 ggagggccag gcgatcaagg ccgggcagct cctccccgag ggcttcttcg gcgtcagcag
     1021 caagctcaag ccggtcggct tcgatccgaa cggggcgaag aagctgctcg ccgaggcggg
     1081 cgtgcccaac ggcttccgca tgacgatcca ctcgcccaac gaccgctacc cgaacgacgc
     1141 caagatcgcc gaggcggtcg ggcagatgct gtcgcgcatc ggcatcgaca cgcaggtggt
     1201 gaccatgacg cagggcgtgt tcttccgcga cgcctccacc gggtcgccgg acaaggggcc
     1261 gaagttcagc ttcatcctgg tgggctgggg ctcgggcacg ggcgaagcct cctcgccgct
     1321 caagtcgctg ctcgcgacct tcgaccgcga caagggcatg ggcgcgtcga accgcggccg
     1381 ctactcgaac ccgcaggtcg acaagctgat caacgacgcg ctcgccacga acgaggacgc
     1441 caagcgcgcc gagctcctcg cgcgcgccac ggagctggcg atcgaggagg tcggcatcat
     1501 cccgctccac taccaggtga acacctgggc gctgcgccgc ggcttcggct acaaggcaag
     1561 gaccgacgag tacaccctcg ccacggaggt ttcgaaggaa aaataatggt tttcatcatc
     1621 aggcgggtcc tgcaaagcct ggtggtcgtg ctggtgatgt cgctggtggt gttcgtgggc
     1681 gtgcatctgg tgggcgaccc ggtgcacatc ctgatctcgg acgacatgac gcagatcgag
     1741 atcgagcgct tcatccagaa gctcggtctc gaccggccgg tgcacgagca gtacttccac
     1801 ttcctcgcca acgccgcgcg cggcgacctc ggcaactcgt tcgtgttcgg cgagccggcg
     1861 ctcaagctca tcctgcagcg catgccggcg acgctcgagc tcgcgttcgc cgccttcctg
     1921 ctcgcgatcg tgctcggcat cccgctcggc atgtacgcgg gcctgcgccc cgagtcgccg
     1981 gcgagcaagg cgatcatggc aggctcgatc ctcggcttct cgctgcccac cttctgggtc
     2041 ggcctgatgc tcatcatggt cttcgcggtg atgctgggct ggctgccctc gaccgggcgc
     2101 ggggacttca tgacctggga cgggctgcgg cacctggcgc tccccgccat caacctcgcg
     2161 ctcttcaaga tggcgctggt gacgcgcctt gcgcgcgccg gcacgcgcga ggcggcgctg
     2221 caggactacg tgaagttcgc tcgcgcgaag gggctctcgc ccgcccgggt cgtgctggtg
     2281 cacgttctca agaacatcct ggtgccggtc gtgacggtgc tcggcctgga actcggcggc
     2341 ctgatcgcgt tctcggtggt caccgaaacg gtgttcgcct ggccggggat gggcaagctc
     2401 ctcatcgact cgatccagcg cctcgaccgc ccggtgatcg tcgcctacct ggtgatcacg
     2461 gtgctgatgt tcgtcgtgat caacctgatc gtcgacatcc tctattcgat gctcgatccg
     2521 cgcgtcaggc tgaaggaagc gaaggcatga gcgccgcggt cgagactctt tccatcgcaa
     2581 aacccgaaac gccgttccgg cgcctcgcgc gcgagttctt cgcgagcccg gtcgccaccg
     2641 gttcgttcgc cgccctgctc atcatcgtgt tcctcgcgct gttcgcgccg ctgctcgcgc
     2701 cgcagaaccc ctacgacctg gcgaaagtca cggtgatgga cgcgcgcctg gcgcccggcg
     2761 aaaccggctc ggcgggcttc atgatgtggc tcggcgcgga cggcgccggg cgcgacatgt
     2821 ggagcgcgat cctctacggg ctgcgcatca gcctcggcgt cggcgtcgcc tcgggagtcg
     2881 tcgcgctcgg catcggcacc gcgctcggcc tcgccgccgc gtaccgcggc ggctggctcg
     2941 acgcgctgat catgcggctc gtcgacctgc agctttcctt cccagcgatc ctggtggcgc
     3001 tgatcctggt ggcgatcctc ggccgcggcg cggacaaggt catgatcgcg ctgatcctcg
     3061 tgcagtgggc ctactacgcg cgcaccgtgc gcggctcggc gctggtcgag cgccgcaagg
     3121 agtacatcga ggccgcgcag tgcctcgcgc tgccgccgcg gcgcatcgta ttccgccatc
     3181 tcctgcccaa cgtgattgca ccgatgatcg tcgtcggcac gctgcagacc gcgcacgcca
     3241 tttcgctcga ggcgacgctg tcgttcctcg gcgtggggct gcccctcacc gagccctcgc
     3301 tcggcctcct catcgcgaac ggcttcgagt acatgctgtc gggcaagtac tggatcagct
     3361 tctatccggg catcgcgctc ctcgccacga tcatgagcat caacctcgtc ggcgaccgcc
     3421 tgcgggacgt gctcaacccg cgccttgaac ggtaataccc tcgaagtcca ggatctcaag
     3481 acccatttct tcacgcgcgc gggcgtggtt cgcgccgtgg acggcgtgag cttcgaagtc
     3541 cgcccgggcg aggtgctcgg cctggtgggc gagtcgggct gcggcaagac ggtgaccggc
     3601 ttctcgatcc tcgggctggt cgacgcgccg gggcgcatca tcggcggccg catcctcttc
     3661 gacggcaccg acctcctgaa gctgggagag cacgagctgc gccggatccg cggcgcgcgc
     3721 atcgcgatgg ttttccagga cccgatgatg acgctcaacc cggtgctgcg catcgacacg
     3781 cagatggtcg aagccgtgcg cgcccatcag gacgtcgatt cgagaatggc attgaagatc
     3841 gctcgtgaag cgctcgagcg cgtcggcatt ccttcgcccg aatcgcgcct gcgctcctac
     3901 ccgcaccaat tcagcggcgg catgcgccag cgcgtggcga tcgcgatcgc gctcctcaac
     3961 cggcccaagc tgatcatcgc cgacgagccc accaccgccc tcgacgtgac gatccaggcg
     4021 cagatcctct acgaggcgca gaagctctgc cgcgagtctg gaaccgccct ggtgtggatc
     4081 acgcacgacc tctcggtggt cgcgggcctg gcggaccgga tctgcgtgat gtatgcaggc
     4141 cgcatcgtcg agcaggggtc ggtcgacgag gtgctcgaca ccccgctcca cccctatacc
     4201 cacggcctga tcggctcggt gccgagccgc aaccggcgcg gcagcccgct ctcccagatc
     4261 ccggggatga tgcccgcgct cgccaacctc ccgccgggct gcgccttccg taaccgctgc
     4321 cctcgcgccg cgtccgcatg cctggaggaa ccaaagcttt cctcccacaa gaatcgtctg
     4381 gtccgctgct tctatccgat gcacgcatga gcgagcccct ggtcgagctg cagaaggtct
     4441 ccaagcgctt cgttcagccg ctcgacttcg ccgggaagat cgcgcgcgcg ctcggcgcgg
     4501 acgtcgcgga cgaagtcgtg catgcggtcg aagacgtgtc gttcgccgtg cacgaaggcg
     4561 aggtcgtcgg cctggtgggc gaatcgggct gcggcaagtc gacgctcggg cgcatggtcg
     4621 cgggaatcct cgagccgagc gccggccgca tccgataccg cggcagggat ggggccggcc
     4681 gtcagagggc cggtctccaa gttcagatga tcttccagga tccgttcgcg tcgctgaatc
     4741 cgcgcatgcg cgtcgccgac atcgtgggcg aggcgccgct ggtgcacggc atcgtcaggc
     4801 gcgaggagct cgacggctac ctcgacgcgc tgctccagcg ctgcggtctc gacccgagct
     4861 acaagccgcg ctatccgcac cagttttccg gcggacagag gagccgcatc ggcatcgcgc
     4921 gcgccctcgc ggtaaagccc gagttcctgg tgtgcgacga ggcggtggcg gcgctcgacg
     4981 tctcgatcca ggcgcagatc ctgaacctct tcatggcgct gcgccgcgac ctggggctga
     5041 cctatctatt catcagccac gacctcggcg tggtcgagca cctctccgac cgggtcgtca
     5101 tcatgtacct gggccgcgtc gtggaatccg cctctacgga tggcattttt gccagagcga
     5161 atcatcccta tacgcaggcg ctgctcaagc aggtgccgcg cctggatacg cgcaagaccc
     5221 agttcaagcc gatcatcggc gagattcctt ctccgctcgc cccgcccacc ggctgccact
     5281 ttcacccgcg ctgcccgcac gccatgccac gctgcaagga cgaagcgccg ccgctgaagg
     5341 aaatcggctc gggccatctc tcggcctgcc acctgaacga ccgctagcag ggcgcgagct
     5401 gcaccaccgg cgcgcgcgag cgcggcagga aatgctccag cggcctgcac cacatttcgc
     5461 cgcagatgac gtagtcctcg cgcaaccccc ggcggtcgaa cacgaggcgc gcttgcggcg
     5521 cgcgcatcgg ccagtagtgc tcccagtcgt cgtcgttgcc gccttgcttc cggattcgcg
     5581 cagcaaagaa ttcgaaatgc ccctgccgca cgtgccacga ttcctcgcgc gccacgccgt
     5641 cggccggcgt ccagccgaag tgcagcgccg cggcgaacag gctggccgtc aggccatcgc
     5701 cggtgaagca caggctactc atagaccgcg gctccaggag tccactgcgc gtcggcgtcg
     5761 agcgggaaca ccggccgcgg caggcccttg aactcgagcc gctccagcac cggcgaagtg
     5821 aggccgggcg tatccacctc gatcacccga tccggaggga atagctcgtc gaagccgccg
     5881 cggaagtggc cgcgcgactt gaccaccacg gtgcgcgccg cgcggatgtc aaggccgtgc
     5941 atctcgaaga acaccggggc ggcgcactgc tttcgcttgc tcacgcacac cacggtaacg
     6001 ccgccgatct gcagcgcgca ggtcgggccg aggtcgagcg cccgcccggc ccagatgccg
     6061 cggcgaccga tgcattgccc gtcggtggtc ttcagaactg tcgccggtgc ccggaagcct
     6121 tccgtgaaga ccgcctcgat ctcgcgcttg cacttcgccg ccgccgcgat ctgcggctcg
     6181 aagaagacgc cgtagagcac ttcgcgcact tcacgttcaa ttaatctctg taacagcgtc
     6241 gtggtttgcc cggtgcctcc cccgcccggg ttgtcgcccg cgtcggacca aatggtcttt
     6301 ccggaaatgc gaagggcgtc ctccatcgac gttagccgct tcacgaaacg cttgcggtcg
     6361 gcccagatgc ggccggcgat atcgagcgcg gtgccgcgaa cgtcggcgcc gcgctcgccg
     6421 ttgatcacga tggcgacccc gtttttcggc gtgtcgctga aaacgaaccc gcccactatg
     6481 gatacgtcga gcaccccgtt gcccaccctg gtctggccgt acctgatcgc gtcggcgtag
     6541 gggccctcgc gcgtcagcag cgtgaccgag gccggggtga gcggcaggcg cacgaacgcc
     6601 ctttttgact tctttccccc gatgaaaccc gtcaggagcc gcgctgcatc caccgcgcgc
     6661 tcggcttggt ccatgtgcgg attcgtgcgg tacgacacga gcgcgtccac gttcgcgacc
     6721 atgcgctcgg agatgttcgc gtgcaggtcg accgtggcaa cgaccggcgt cctcggcccg
     6781 gcggccccgc gcaccatcgc atagagctcg ccgtcggggt ccgggccgcc ggtggagatc
     6841 atgccgccgt ggttgctgat gtagaccgcg tccagctttc ccgcggcctg caggcgagcg
     6901 cgcatcgcgg cgagggtcgc ctggaaaaag cgctcgtcca ccgcgccgcc tggctcgcag
     6961 gcggtgacga cgatcgggac cggcgtccac gggccgagcg catccatttc gccgtagaac
     7021 cccggaatct cggccggaag cgccgatacc ttcttgcgcg cctcgcggct gatctcttcg
     7081 ccggcgcagt agcagagcga gcggaagtgc cgctcttcgc tgaccgcagc gaacgcgttg
     7141 ctctccaggt gcaagccgag aacggcgacc cgtgggcccg taacttgctg ggatttcaac
     7201 gttggtccaa cctaggaggt ctcatgaagc gatcaatcgc gctgatgttc atcccggccc
     7261 tgctgctcgc cggctgcagc cgcccagtgg tcaaggaaac cgtcgtcgag cgacccgtag
     7321 tggtcgaacg tgcggcagcc acgggcgcga gcgcgccgag ctgcacgtac gcgtcgcagg
     7381 cttacagcca cggcagcgtg tcctgccagg accgcaccca gttccgctgc gacaacggca
     7441 tctggaaccg cacgttcacg gcgtgctagc agcgctcttc gaggccattc agggacaggt
     7501 cattccgctt cctcgatcca ggcttgctgg atggcctcga ggattctttc cccgcagcgc
     7561 ttcgggtcgt cgctgaaccc cggcagcgcc agcacccact tgtagaggtc gacgaagttc
     7621 accgacgtcg gatccttgtc gggatggctt tccgacaggg cgatcgcaat gtcctgcgta
     7681 tcggtccact tcatttcttg gctcccccgc cctcgcgctc gtagttgatc gtgtatttgg
     7741 ggatctcgat caccagatcc tggtccgcga ctttcgcctg gcacgacagg cgcgacgtcg
     7801 cctcgaggcc ccaagccttg tcgagcaggt cttcttcctt ttcctcgggc ggctcgagcg
     7861 aatcgtagcc ttggcgcacg atcacgtgac aggtagtgca ggcgcacgac ttctcacagg
     7921 catgctcgat gtcgatatgc cgctcgagca gcgtatcgca gatcgacacc ccgcttctcg
     7981 cctcgatctc cgcaccctgc gggcaaagcg catgccccgg taaaaccttg atcttcgcca
     8041 tcagagactg ccgatcctgc ggccggcgag cgcgcgcttg atcccctcgt ccatgcgccg
     8101 cgccgcgaac tcgtcggtgg cgcggttcag cgcctcgacc gcctgcctga tggcgcggtg
     8161 ctccgtgccg ggaagcagtt tttcgaggga ggcgatggcg gcgtcgattg ccgcacgctc
     8221 ttcggcattc aacaacttcg cgtccgcgac caacgccgag cgcgtagcct cgatcaaccg
     8281 ctggccgtcg acccgctgct ccgcgagggc gcgggcatgc gtgtcctccc gggcgtgctc
     8341 gaaggaatcg cgcagcatgc gctcgatgtc ggcgtcgccc agtccgtagg aaggcttgac
     8401 cgtgacggag gcttccaccc cggaggtgcc ttctttggcg gtaacggaga gaagcccgtc
     8461 ggcgtcgacc tggaacgcga cgcggatgcg cgcggcgccg gcgaccatcg gcgggatgcc
     8521 cttcagctcg aagcgcgcca gcgagcggca gtcggacacg agctcgcgct cgccctgcac
     8581 gacgtggatg ctcatcccgg tctggccgtc cttgaaggtg gtgaactcct gcgcccttcc
     8641 ggccgggatg gtggtgttgc gcgggatgat cttctcggtg aggccgccca tggtctcgat
     8701 gccgagcgac agtgggatca cgtcgagcag cagccagtcc tcgccctgcg gcttgttgcc
     8761 ggc
//
LOCUS       DATBSZ010000146         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_158391, whole genome shotgun sequence.
ACCESSION   DATBSZ010000146 DATBSZ010000000
VERSION     DATBSZ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158391"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VIG70_09820"
     CDS             <1..561
                     /locus_tag="VIG70_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8250893.1"
                     /translation="PGFAIGSLLGAVLGLAAGTARWFDRMLSPAVFLTYPVPKIVMLP
                     IFMIWFGIGDLSKVLIIALACFYPVFINAYYGVRQTRTILVWSALNMGATQGEIFRRV
                     VLPSAAPMIFAALRVSLALSFIVMFAAEMINARSGLGHLIRIAENSLRFDLMYVSLVT
                     IAILGYAGDRLVRLAHSRLLAWQQPA"
     gene            558..1316
                     /locus_tag="VIG70_09825"
     CDS             558..1316
                     /locus_tag="VIG70_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8250894.1"
                     /translation="MSRLLPFVLVGIAMAAWEVAARSGLWSPLLFPSLEKIFRELGLF
                     FTKTEMLAEIQVSLERALGGFALAAAAGILLGLVMGRSRFAAGLLDPLFSGTYAVPKL
                     ALFPIFIFVFGIGSLSKVALVFLECLYPIVIMTYAGARDVNRALLWSAQNMGASRAAI
                     LRRVVIPATTPFIFAGFRVAVPVAMIVVVITEMVASADGLGYQVIYAMSSLRTDRMLA
                     VVIVIAALGYALDKAVVLLRDRLIYWEKLDTYYV"
     gene            1327..>2129
                     /locus_tag="VIG70_09830"
     CDS             1327..>2129
                     /locus_tag="VIG70_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8250895.1"
                     /translation="MKAVAGFFAFLFALNAVAQTAPLIRLGRGFAAEEQLWLMSARPD
                     LTPNQGKRYQLKQILFQANPERFQAYLAGELDAGTAPGLAIIFGRSQGMDFKLVAGVC
                     QEAAGSQYFTTVYMVKDDGPIKSVKDLKGGTIGVVGIRTATDLWARAGLLNAGLVPDK
                     DTKVVPMAFPVIGDAVRTGKVSAGTFVEPFYSAEMAKGGLRKLFTATEALGYDHELLD
                     IFFSEKFLKANPDAVRAFLADYVAVTKYYLANTEQAKTDLHKAGFVRTPL"
BASE COUNT          307 a          745 c          673 g          404 t
ORIGIN      
        1 cccggcttcg cgatcggcag ccttctcggc gccgtgctcg ggctcgccgc gggcaccgcg
       61 cgctggttcg accgcatgct gagccccgcc gtgttcctca cctacccggt gccgaagatc
      121 gtgatgctgc cgatcttcat gatctggttc ggcatcggcg acctgtcgaa ggtcctcatc
      181 atcgcgctcg cgtgcttcta tccggtgttc atcaacgcct actacggcgt gcgccagacg
      241 cgcaccatcc tggtgtggtc ggcgctcaac atgggcgcca cgcagggcga gatcttccgc
      301 cgggtggtgc tgccgagcgc cgcgccgatg atcttcgcgg cgctgcgcgt gtcgctcgcg
      361 ctctcgttca tcgtcatgtt cgccgccgag atgatcaacg cgcgctccgg cctcggccac
      421 ctgattcgca tcgcggaaaa ctcgctgcgc ttcgacctga tgtacgtgtc gctcgtgacc
      481 atcgcgatcc tcggctacgc cggcgaccgg ctggtgcgcc tcgcgcattc gcgcctgctt
      541 gcctggcagc agcccgcatg agccggctct tgccgttcgt tctggttgga atcgcaatgg
      601 cggcgtggga agtggccgcc cgcagcggcc tgtggagccc gctgctcttc ccctcgctcg
      661 agaagatctt ccgcgagttg ggattgtttt tcaccaagac cgaaatgctg gccgagatcc
      721 aggtctcgct cgagcgggcg ctcggcgggt tcgccctcgc cgcggcggcc ggcatcctgc
      781 tcggcctcgt gatgggccgc tcgcgcttcg cggctgggct gctcgacccg ctcttctccg
      841 gcacctatgc ggtgccgaag ctggcgctgt tcccgatctt catcttcgtg ttcggcatcg
      901 gcagcctgtc gaaggtcgcg ctggtgttcc tcgagtgcct ctacccgatc gtgatcatga
      961 cctacgccgg cgcgagggac gtcaaccgcg cgctcctctg gtcggcgcag aacatggggg
     1021 cctcgcgcgc agcgatcctg cgccgcgtgg tgatcccggc gaccacgccg ttcatcttcg
     1081 cgggctttcg cgtcgcggtg ccggtggcga tgatcgtggt ggtgatcacc gagatggtcg
     1141 cctcggccga cggcctcggc taccaggtga tctacgcgat gtcctcgctc aggaccgacc
     1201 gcatgctcgc ggtcgtgatc gtgatcgcgg cgctcggcta cgcgctcgac aaggcggtcg
     1261 tcctgctgcg ggaccggttg atatactggg aaaagctcga cacctattac gtctgagggg
     1321 gattccgtga aagcagtcgc tgggtttttc gccttcctgt ttgccctgaa tgcggttgcg
     1381 caaaccgccc ccctgatccg ccttggccgc ggcttcgccg ccgaggagca gctctggctg
     1441 atgagcgcgc gccccgacct cacgcccaac cagggcaagc gctatcagct gaagcagatc
     1501 ctcttccagg ccaacccgga gcgcttccag gcctacctcg ccggcgagct cgacgccggc
     1561 accgcgcccg gcctcgccat catcttcggc cgctcgcagg gcatggactt caagctcgtc
     1621 gcgggcgtat gccaggaagc cgcgggcagc cagtacttca ccaccgtcta catggtcaag
     1681 gacgacggcc cgatcaagag cgtcaaggac ctgaagggcg gcaccatcgg cgtcgtcggt
     1741 atcaggaccg cgacggatct ctgggcgcgc gccggcctgc tcaacgcggg cctggtgccc
     1801 gacaaggaca ccaaggtcgt gccgatggca tttccggtga tcggcgacgc cgtgcgcacc
     1861 ggcaaagtca gcgcgggcac cttcgtcgag cctttctatt cagccgagat ggccaagggc
     1921 gggctgcgca agctcttcac cgcgaccgag gccctgggct acgaccacga gctcctcgac
     1981 atcttcttca gcgagaaatt cctgaaggca aatccggacg cagtgcgcgc cttcctcgcc
     2041 gactacgtcg cggtcaccaa gtactacctc gccaacacgg agcaggcgaa gaccgacctg
     2101 cacaaggccg gcttcgtgcg cacgccgct
//
LOCUS       DATBSZ010000147         7021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1319608, whole genome shotgun sequence.
ACCESSION   DATBSZ010000147 DATBSZ010000000
VERSION     DATBSZ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319608"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3269
                     /gene="rpoC"
                     /locus_tag="VIG70_09835"
     CDS             <1..3269
                     /gene="rpoC"
                     /locus_tag="VIG70_09835"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY8250896.1"
                     /translation="ITGTNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGP
                     ELKLHQCGLPKKMALELFKPFIFNKLELLGLATTIKAAKRLVEQEVAEVWDILEEVIR
                     EHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAFNADFDGDQMAVHVPLSLE
                     AQMEARTLMLASNNVLSPANGEPIIVPSQDIVLGLYYATRERINAKGEGMSFSDLAEV
                     HRAYDSRQVELNARVWVRIKEKELSESGEVTEKITRYETTIGRALLSEILPMGLSFEL
                     LNKALKKKEISKLINIGFRRCGLRETVIFADKLMQAGFGIATRAGISFGVHDMTIPPQ
                     KQELIKQAEGEVKEIENQYTSGLVTAGERYNKVVDIWSRCGEQVGKVMMEQLGSEEVE
                     NRHGKKVKQEAFNSTYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFRE
                     GLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVTQDLVVTEEDCGTANGVSMK
                     ALVEGGEVVEALRERILGRTLATDLLHPETQDVLYAAGTLIDEEGVDAIDNVGIDEVK
                     VRTALSCDTRWGVCAKCYGRDLGRGSPVNVGEAIGVIAAQSIGEPGTQLTMRTFHIGG
                     AASRTAVQSHVEAKSSGTVRFTSTMRYVSNAKGDKVVISRSGEVTIVDDNGRERERHK
                     VPYGALLAVADGDSVRAGKQLATWDPHHRPIITEYAGTVKFEHVEEGATVAKQIDDVT
                     GLSTLVVIDAKRRTTAAGKGQRPSVKLINEVGDEVRIAGTDHAVNISFPVGAVIAVRD
                     GQQVQVGEVLARIPQEVSKTRDITGGLPRVAELFEARSPKDAGMLAEVTGTVSFGKDT
                     KGKQRLVITDLDKQEHEYLIAKDKHLLVHDGQVVNKGEMIVDGPVDPHDLLRLLGVEE
                     LARYIIDEVQDVYRLQGVKINDKHIEVIVRQMLRRVEITEPGDAPFITGEQAERAEVL
                     EENEKAEKAGKKPAQYQYMLLGITKASLSTDSFISAASFQETTRVLTEAAIMGKRDGL
                     RGLKENVIVGRLIPAGTGLAYHMIRKAQAAEPVLAELPAAAPEAPPAAETTATAGGGS
                     "
     gene            3443..4489
                     /locus_tag="VIG70_09840"
     CDS             3443..4489
                     /locus_tag="VIG70_09840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY8250897.1"
                     /translation="MRSLLAKSWLLSIALVLALLAPDPASADGFFRHRPIVVFGDSLS
                     DPGNAFAVNGGQTVAPPDYGMGGVDAAGIPEVIALIPEAPYATRHFSNGVTWIELLAG
                     TFGLGSSVKPALAGADRRASNYAVGGATAADVGASPFHLSVQVDFFLRDVRNDAPSHS
                     LYVVAIGGNDIRAAMAAALAGQDPNRVIGAALASVAGNIGKLHHAGARKFLVWNAPDL
                     GRTPALQRLNALVAPGIAGLATALSAGYNQNLAVVLQGLEALPGIEIVQFDAFALLAD
                     VQANPAKFGLRDATTACIQPHVPMFGFPSAPPFRCALPDRHFFWDGIHPTRAGHAIIA
                     FLVGKTLVTEALEH"
     gene            4563..5135
                     /gene="folE"
                     /locus_tag="VIG70_09845"
     CDS             4563..5135
                     /gene="folE"
                     /locus_tag="VIG70_09845"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY8250898.1"
                     /translation="MTPNIQKIETLVRQLLIELGEDPGREGLQKTPLRVAQALGFLTQ
                     GYRTDLARVINNALFTQDTSSMVIVKDIEMYSLCEHHMLPFFGRCHIGYIPNGKVFGV
                     SKLARLVDMHARRLQLQERLTDDISREIMNSIDAKGVGVMIEARHLCMMMRGVEKQNS
                     TMVTSSVLGVFRDHLATREEFLSLVARRTA"
     gene            5153..5968
                     /locus_tag="VIG70_09850"
     CDS             5153..5968
                     /locus_tag="VIG70_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY8250899.1"
                     /translation="MLGVLLWFAAAHAELRPEWEFGLGASAFTLPDYRGSDESRGYVL
                     PFPYVIYRGDRVRVDRQGVRGIFFESDRVELNLSVNATPPVDSDDNRAREGMPALDPT
                     LEIGPRLDITLARDRAREWSFELRLPLRAAIATDLSHTRHIGWVAYPHLHLFTRPELA
                     GVKWNLGLQAGPLFATRKYHDYFYAVDPEFAAAGRPAYEAPGGYSGALALLSLTRRFP
                     RFWVGAFARYDTLKGAAFESSPLVRRDYAVMAGFAIAWVFTESDRKVEVDVDE"
     gene            6059..6181
                     /locus_tag="VIG70_09855"
     CDS             6059..6181
                     /locus_tag="VIG70_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250900.1"
                     /translation="MERLQQWRRKQEGKLGYILLWALGVPIPVLFVIFLMRGCT"
     gene            6226..6465
                     /locus_tag="VIG70_09860"
     CDS             6226..6465
                     /locus_tag="VIG70_09860"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEY8250901.1"
                     /translation="MSKVLVAFGIVLATGLAGCKALDDRGVSRETQGTVGGAVAGGAV
                     GSAVTGGSAIGTIGGAVVGGAVGNEMGERYDEKRK"
     gene            6529..>7021
                     /locus_tag="VIG70_09865"
     CDS             6529..>7021
                     /locus_tag="VIG70_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY8250902.1"
                     /translation="MGVQELEVTKGVERAMRIDLAAAFRLAVRLDLHEGVCNHLSVML
                     PDGKRFLLNRYGLHWSEVSASNLLMLDASGNVLEGEGEFEKTAFYIHSRIHLANPRAT
                     CVLHTHMPYATALTLLEGGRLEMVEQNALRFHDDIAYDDIYNGLVVDNAEGDRLARAL
                     GGKR"
BASE COUNT         1246 a         2282 c         2351 g         1142 t
ORIGIN      
        1 tcatcacggg cacgaacaag cgtccgctga agtcgctgtc cgacatgctg aagggtaagc
       61 agggcaggtt ccgtcagaac ttgctcggca agcgcgtcga ctattcgggc cgttcggtca
      121 tcgtcgtggg tccggagctc aagctccacc agtgcggctt gcccaagaaa atggccctcg
      181 agctcttcaa gcctttcatt ttcaataagc ttgaactcct cgggttggcc acgacgatca
      241 aagcggcaaa aaggctcgtg gagcaggaag tcgcggaggt gtgggacatc ctggaagagg
      301 taatccgcga gcacccggtg ctgctcaacc gcgcgccgac cctgcaccgc ctcggcatcc
      361 aggcgttcga gccggtgctg atcgagggca aggcgatcca gctccatccg ctggtgtgcg
      421 cggcgttcaa cgccgacttc gacggcgacc agatggcagt ccacgtcccg ctttctctcg
      481 aggcgcagat ggaagcgcgc acgctgatgc tcgcctccaa caacgtgctt tcgcccgcta
      541 acggtgagcc gatcatcgtg ccttcgcagg acatcgtgct cggcctgtac tacgccacgc
      601 gcgagcgcat caacgcgaaa ggcgagggca tgagtttctc cgacctcgcc gaagtgcacc
      661 gcgcctacga ctcgcgccag gtggagctga acgcgcgcgt ctgggtgcgc atcaaggaaa
      721 aggagctgtc cgagtccggc gaggtgaccg agaagatcac gcgctatgag acgacgatcg
      781 gccgggcgct gctttccgaa atcctgccga tgggcctgtc gttcgagctg ctcaacaagg
      841 cgctcaaaaa gaaagagatc tcgaagctga tcaatatcgg ctttcgccgc tgcgggttgc
      901 gcgagacggt aatcttcgcc gacaagctga tgcaggcggg cttcggcatc gccacgcgcg
      961 cagggatttc cttcggcgtg cacgacatga ccatcccgcc gcagaagcag gagctgatca
     1021 agcaggctga gggcgaggtg aaggagatcg agaaccagta cacctcgggc ctggtgaccg
     1081 ccggcgagcg ctacaacaag gtggtcgaca tctggtcgcg ctgcggcgag caggtcggca
     1141 aggtgatgat ggagcagctc ggctccgagg aggtcgagaa ccgccacggc aagaaggtga
     1201 agcaggaggc gttcaactcg acctacatga tggccgattc gggcgcgcgc gggtcggcgg
     1261 cgcagatccg gcagctggcc ggcatgcgcg gcctgatggc caagcctgac ggctcgatca
     1321 tcgagacccc aatcactgcc aacttccgcg aagggctgaa cgtgctgcag tacttcatct
     1381 ccacgcacgg cgcgcgcaag ggcctcgcgg atacggcact gaagacggcg aactcgggct
     1441 acctcacgcg ccgcctggtc gacgtgacgc aggacctggt ggtgaccgag gaggattgcg
     1501 gcaccgcgaa cggcgtttcc atgaaggcgc tggtcgaggg cggcgaggtc gtcgaggcgc
     1561 tgcgcgagcg catcctcggc cgcacgctcg ccaccgatct cttgcatccc gagacgcagg
     1621 acgtgctcta cgcggccgga acgctgatcg acgaggaggg cgtggacgcc atcgacaacg
     1681 tcggcatcga cgaggtcaag gtcaggaccg cgctttcctg cgatacgcgc tggggcgtgt
     1741 gcgccaagtg ctacgggcgc gacctcggac gcgggtcacc ggtgaacgtg ggcgaggcga
     1801 tcggcgtgat cgccgcgcag tcgatcggcg agccgggcac gcagttgacg atgcggacct
     1861 tccacatcgg tggcgccgcc tcgcggaccg cggtgcagag ccatgtcgag gcgaagtcat
     1921 ccggcacggt ccggttcacc tcgactatgc ggtacgtgtc gaacgcgaag ggcgacaagg
     1981 tggtgatcag ccgctcgggc gaagtcacca tcgtcgacga caacggccgc gagcgcgagc
     2041 gccacaaggt gccgtacggc gcgctgctcg cagtcgccga cggcgacagc gtgcgcgcgg
     2101 gcaagcagct cgccacctgg gacccgcacc accggccgat catcacggag tacgccggca
     2161 cggtgaagtt cgagcacgtg gaggaaggcg cgaccgtcgc caagcagatc gacgacgtca
     2221 cgggcctctc gaccctcgtc gtcatcgacg ccaagcggcg caccaccgcc gcgggcaagg
     2281 gccagcggcc ctcggtgaag ctgatcaacg aggtgggcga cgaggtgcgc atcgccggca
     2341 ccgaccacgc cgtgaacatc agcttcccgg tgggggcggt gatcgcggtg cgcgacggcc
     2401 agcaggtgca ggtgggcgag gtgctcgccc gcattccgca ggaagtctcg aagacgcgcg
     2461 acatcaccgg cggcctgccg cgggtggcgg agctgttcga ggcgcgctcg ccgaaggacg
     2521 cgggcatgct tgcggaagtc accggaaccg taagcttcgg taaggacacg aaaggcaagc
     2581 agcgcctggt gatcaccgac ctcgacaagc aggagcacga gtacctgatc gccaaggaca
     2641 agcacctgct ggtgcacgac ggccaggtgg tgaacaaggg cgagatgatt gtcgacggcc
     2701 cggtggatcc gcacgatctg ctgcgcctgc tcggcgtcga ggagctcgcg cgctacatca
     2761 tcgacgaggt gcaggacgtg taccgcctgc agggcgtgaa gatcaacgac aagcacatcg
     2821 aggtgatcgt gcggcagatg ctgcgccgcg tggagattac cgagccgggc gacgcgccgt
     2881 tcattaccgg cgagcaggcc gagcgcgccg aagtgctcga ggagaacgag aaggccgaga
     2941 aggcgggcaa gaagccggcg cagtatcagt acatgctgct cggcatcacc aaggcctcgc
     3001 tctccaccga ctcgttcatc tcggcggcct ccttccagga gaccacgcgc gtgctgaccg
     3061 aggcggcgat catgggcaag cgcgacggcc tgcgcggcct caaggagaac gtcatcgtcg
     3121 gccgcctgat cccggcggga accggcctcg cgtatcacat gatccgcaag gcgcaggccg
     3181 ccgagccggt gctggccgaa ttgccggccg ccgcgccgga ggcgccgccc gcggcggaga
     3241 ccacggcgac cgccggaggc ggcagctagc cttctttgcc aagtcccaag agggcgcctg
     3301 cgggcgccct tttttttcca ccattcgatg gaacaggcga cgcaacgatt agtacgcttt
     3361 gctcatggga ttgacatccc tcactcctgt cactagcttg gtccgctagc ctcgcgagag
     3421 gcgtctctgg gaggagaccg tcatgcgttc gctgctcgcc aagtcgtggc tgctttcgat
     3481 cgctctcgtg ctggcgctgc ttgcgccgga ccctgcgtcc gcggacggct tctttcgcca
     3541 tcggccgatc gtcgtgttcg gcgacagcct gtcggatccg ggcaacgcgt ttgcggtgaa
     3601 cggcgggcaa accgtcgcgc cgcccgacta tggaatgggc ggggtcgatg cggcgggaat
     3661 ccccgaggtc atcgcgctca ttcccgaggc gccctacgcg acgcgccatt tcagcaacgg
     3721 cgtgacatgg atcgagcttc tcgccggcac cttcggcctg ggctccagcg tgaagccggc
     3781 gctcgccggc gcggatcgcc gggcgtcaaa ctacgcggtc ggcggcgcga ccgcggccga
     3841 cgtcggcgcg agcccgttcc acctcagcgt gcaagtggac ttcttcctgc gcgacgtcag
     3901 gaacgatgct ccctcccact cgctctacgt cgtcgccatc ggcggcaacg acatccgcgc
     3961 cgccatggcg gcggccctgg cgggccagga tcccaacagg gtgatcggcg ccgcattggc
     4021 ttcggtcgcg ggcaacatcg gcaagctgca ccacgcgggc gcgcgcaagt tcctggtctg
     4081 gaatgctccc gatctcggcc gtacgcccgc gctgcagcgc ctcaatgcgc tggtggcgcc
     4141 gggaatcgcc ggcctggcca ccgccctgag cgcggggtac aaccagaatc tcgcagtcgt
     4201 tctgcagggg ctcgaagcgc tgccgggcat cgaaatcgtg cagttcgatg cgttcgccct
     4261 gctcgcggac gtccaggcga acccggcgaa attcggcctg cgcgacgcca ccaccgcgtg
     4321 catccagccc cacgtgccga tgttcggttt ccccagcgcg ccgccattcc gctgcgcgct
     4381 gcccgaccgc cattttttct gggacgggat tcacccgacg cgggcggggc acgcgatcat
     4441 cgcttttctg gtgggcaaga cgctcgtgac cgaagccttg gagcactagc ggcaagagca
     4501 ggcttttctc gtaccatggc aagggcgcct tcgggcgccc ttctcatttc atgagccctg
     4561 ccatgacacc gaatatccag aagatcgaaa ccctggtgcg ccagctgctg atcgagctgg
     4621 gcgaggatcc cggccgagag gggctgcaga agaccccgct gcgggtagcg caggccctcg
     4681 gctttctcac ccagggctac cgcaccgacc tcgcaagagt catcaacaac gcgctcttca
     4741 cgcaggacac gagcagcatg gtgatcgtga aggacatcga gatgtacagc ctgtgcgagc
     4801 accacatgct gccgtttttc ggccgctgcc acatcggcta catcccgaac gggaaggtgt
     4861 tcggcgtgag caagctcgca cgcctggtcg acatgcacgc gcggcgcctg cagctgcagg
     4921 agcgcctgac cgacgacatc tcgcgcgaga tcatgaactc catcgacgcc aagggcgtcg
     4981 gcgtgatgat cgaggcgcgc cacctgtgca tgatgatgcg cggcgtggag aagcagaatt
     5041 cgaccatggt cacctcctcg gtgctgggcg ttttccgcga ccacctcgcc acgcgcgagg
     5101 aattcctgag cctggtcgcg aggaggacgg catagctctg cgcggttgct gtgtgctggg
     5161 ggtcctgctg tggttcgcgg cggcgcacgc cgagctgcgc cccgaatggg aattcgggct
     5221 gggagcgagc gcattcacgc tgcccgacta tcgcggctcg gacgagagcc gcggctacgt
     5281 gctgccgttc ccctatgtga tttaccgcgg cgaccgcgtg cgcgtcgacc gccagggcgt
     5341 gcgcgggatt ttcttcgagt ccgaccgcgt cgagctcaac ctcagcgtga acgcgacgcc
     5401 gccggtggac agcgacgaca accgtgcgcg cgagggcatg ccggccctcg atcccacgct
     5461 cgagatcggc ccgcgactgg acatcaccct cgcgcgcgat cgcgcgcgcg aatggtcgtt
     5521 cgagctgcgc ctgccgctgc gcgcggcgat cgcgaccgat ctgtcgcaca cccggcacat
     5581 cggttgggtg gcgtatccgc atctgcatct tttcacgcgg ccggagctcg ccggggtcaa
     5641 gtggaacctc ggattgcagg ccgggccgct ctttgccacg cgcaagtacc acgattattt
     5701 ctacgccgtc gatcccgagt tcgccgcggc cggacgcccc gcgtacgaag cgcccggcgg
     5761 ctattcggga gcgcttgcgc ttctgtcgct tactcggcgc tttccgcgat tctgggtggg
     5821 cgccttcgca cgctacgaca cgctgaaggg cgcggccttc gagtcgagcc ccctggtgcg
     5881 gcgggactac gccgtgatgg cgggcttcgc catcgcctgg gtgttcaccg agtccgaccg
     5941 gaaagtcgaa gtcgacgtgg acgaatgacg tagtggaatt ccccacaagg ggaagcgaca
     6001 gcggcacggc gcttgctcct ccctgcgatg tccgcatacg gatcgcttgg aggtagtcat
     6061 ggaaaggctg cagcagtggc gccgcaagca ggaaggcaag ctcggttaca tcctgctgtg
     6121 ggcactaggc gtgcccatcc cggtgctgtt cgtcattttc ctaatgcgcg gctgcacctg
     6181 agcgcaagca cggttctcga ccttttttca aacggaggcc atcacatgag caaagttctt
     6241 gttgcattcg gcatcgttct cgcgaccggc ctggccggct gcaaggcact cgacgaccgc
     6301 ggcgtctcgc gtgaaacgca aggcaccgtc ggcggcgccg tcgccggcgg agccgtcggc
     6361 tccgcagtga ccggcggcag cgcgatcggc acgatcggcg gcgcggtggt cggcggcgcg
     6421 gtcggcaacg aaatgggcga gcgctacgac gagaaacgca agtagccttt tcgccgttct
     6481 cgaaaagggc gccttcgggc gccctttttg tttcatataa tgggcttcat gggagtccaa
     6541 gaactcgaag tcaccaaagg ggtggaacgc gcgatgcgca tcgacctcgc ggcggccttc
     6601 cgcctcgcgg tacgcctgga cctgcacgaa ggggtctgca accacctaag cgtcatgctg
     6661 cccgacggca agcggtttct actgaaccgt tacgggctgc actggagcga ggtcagcgcc
     6721 tctaacctgc ttatgctaga cgccagcggc aacgtcctcg aaggcgaagg cgagttcgag
     6781 aagaccgcgt tctatatcca ttcgcgcatc catctcgcca acccgcgcgc gacctgcgtg
     6841 ctgcacacgc acatgccgta tgccaccgcg ctcacgctgc tcgagggcgg caggctggag
     6901 atggtggagc agaacgcgct gcgctttcac gacgacatcg cctacgacga catctataac
     6961 gggctggtcg tcgacaacgc cgagggcgat cgcttggcgc gggcgctcgg cggcaagcgc
     7021 g
//
LOCUS       DATBSZ010000148         6488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1797980, whole genome shotgun sequence.
ACCESSION   DATBSZ010000148 DATBSZ010000000
VERSION     DATBSZ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797980"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VIG70_09870"
     CDS             <1..556
                     /locus_tag="VIG70_09870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8250903.1"
                     /translation="REAGARVVFEPVNQIARARNAGAARASGEWLVFVDADSYPSREL
                     FAEAAAAIRGGRALGGGATVAFEIRDPVIRLSLAIWNAVSRAARWTAGSFVFCEAAAF
                     RSLGGFSEALYAGEEIDFARRLKRLARSRGLGVVILHRHPLRTSDRKARLYTKREHFA
                     FLLKTLLLAGRTLRPQRVLRLVRR"
     gene            complement(631..1149)
                     /locus_tag="VIG70_09875"
     CDS             complement(631..1149)
                     /locus_tag="VIG70_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEY8250904.1"
                     /translation="MRRRELLGAALLLPLEGLAQTCGVLTPRQTEGPFFTPKSPQRVS
                     LLEGGEKTRIFVIGTVLSAQCNPVPNALLDFWHSDELGEYDNRGFRYRGHQHADAQGR
                     FRLETIVPAEYPGRTRHIHVKVQAPGRRILTTQLYFPGDPGNRRDGLYRAELEMKKAK
                     ADEASFDFVVET"
     gene            complement(1146..2342)
                     /locus_tag="VIG70_09880"
     CDS             complement(1146..2342)
                     /locus_tag="VIG70_09880"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEY8250905.1"
                     /translation="MPVRQEIAEGLVPVKVYTRDLEPGARQQLVSLSRLPIVHHHVAA
                     MPDVHRGIGATVGSVIPTRKAIIPAAVGVDIGCGMTATRVSLGADQLDEASLKKLFAQ
                     ISRDVPAGFGQHSDREARSHAAKRFQKQLTKIFEKHPGVERRCSKHSSWVNQLGTLGG
                     GNHFIEICLDEENRVWAMLHSGSRGIGNAIGSYFIELAKKDAQRNNVQLPERDLAYFR
                     EGSVHFDDYIEAVGWAQEYARANREEMMDLVLSAMQRHLPASEVTEGVVNCHHNYVER
                     EEHYGEQVWLTRKGAIRAGAGELGIIPGSMGVRSYIVRGKGSPESFDSCAHGAGRLMS
                     RNAAQKKFSVKDLEDQTHGVICRKDKGVLDEIPGAYKPIDEVMENQADLVDIVHTLKQ
                     VICVKG"
     gene            complement(2533..3393)
                     /locus_tag="VIG70_09885"
     CDS             complement(2533..3393)
                     /locus_tag="VIG70_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8250906.1"
                     /translation="MNVKELVAIDVHTHAWKSALQVDDKLTESQEAMGRYFRYQPQHA
                     TVPEMADYYRKMKMGFVVFTVDSPRDNRKITNEEIAELAIKNGDVAIAFASINPHRGA
                     DGVRAAKRLIAEYKVKGFKFHPTVQEFYPNDRLAYPLYEVIAEAKLPALFHTGQTGMG
                     AGTRGGGGLRLKYSNPLYLDDVAADFPDMPIVLAHPSFPWQEEALSVATHKPQVFIDL
                     SGWSPKYFPPILVQYANTLLKDKVLFGSDYPVITPEKWIEEFDKLPIKPEVRPLILKE
                     NAARLLRLAQ"
     gene            complement(3390..5132)
                     /locus_tag="VIG70_09890"
     CDS             complement(3390..5132)
                     /locus_tag="VIG70_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HEY8250907.1"
                     /translation="MSQPQHARRTRSVRLARQTVLAERRAGNVYLRSPDPLPAYPRKL
                     TERLDHWAEHAPERVFLAQRGPDGVWQRLTYAQARSRARRIAQALLERRLSAERPVAV
                     LSGNDFAHALIELGALYAGVPYAPVSPAYSLLSKDFAKLKHVMRLLTPGLVFASVREP
                     FQAAIAAAVPGGTEVVYEVQKLEAGEATGAVDEAHARVGPDTIAKFLFTSGSTGEPKA
                     VINTQRMWCSNQEMIRFMFAFFEEEPPLVVDWAPWHHTAAGNHDFGLVLYNGGSYYID
                     EGKPLPGAIETTVRNLREIAPTWYFNVPRGYEVLLPFLRTDLGLRKNFFSRLKLLWFA
                     GAAVSQPVFDEVKALAYETCGEEILFLTGLGSTETAPFAMGRMWETKDVTNMGLPPPG
                     VEIKLVPLQDKLEARLKGPNITPGYWRQPELTAQVFDEEGYYRLGDSFVLANEQKPVE
                     GLLFRGRIAEDFKLSSGTWVNVGPLRALFIAHFAPLVRDVVIAGEGKSEVGVLIFPAG
                     NAPADELLKRLKTLASTGSSNRIARALVLEEPPSLDAGEMTDKGSINQRAVLRRRAAL
                     VEELYTDSPRVLLT"
     gene            complement(5129..6106)
                     /locus_tag="VIG70_09895"
     CDS             complement(5129..6106)
                     /locus_tag="VIG70_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HEY8250908.1"
                     /translation="MQTRRRFLGTAALGVLGGALPFAVRGQGIDTAKIIVGFPPGGTT
                     DVMARKVADKLRGAYARTAIVENRPGAGGQLGVVALKDAPPDGATLLLTPSSMLTIYP
                     NTYPKLQYRVEDVAPVSLAMYSHHVLSVGPAVPDSVKTLKDFFAWAKANPDKAVYGSP
                     GAGSMPHLIGVLLDKAAGANLRHAGYRGSAPGIQDLLGGQIASFLGPTGDVLAHSQAS
                     KVRVLGTSGKERSAFLPAVPTMGEQGFKIALREWYAFFMPARASAETVQRASAALQAV
                     LGQAEVVEFAKQFGLEVQGSSPAQLAEVFKADTAEWGGLVKQTGFTAES"
     gene            complement(6115..>6488)
                     /locus_tag="VIG70_09900"
     CDS             complement(6115..>6488)
                     /locus_tag="VIG70_09900"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250909.1"
                     /translation="WARLEAACAAHLTLLVGEEYESLVGGTSLFHPARSPLQRRLNRD
                     RDVYEERFRQMIAALELPPDVDQTLLRLNLLGALNWTHNWYRSGRKSPRELAHQLVQK
                     ILRRSLEAPGRRAESRKVVLS"
BASE COUNT         1024 a         2183 c         2134 g         1147 t
ORIGIN      
        1 gcgcgaggcg ggcgcgcgcg tagtgttcga gccggtgaac cagatcgcgc gcgcgcgcaa
       61 tgccggcgca gcacgggcga gcggcgagtg gctggtattc gtcgacgccg actcgtatcc
      121 cagccgcgag ctgttcgccg aggcggcggc cgcgatccgc ggcgggcgcg ccctcggcgg
      181 cggcgccacg gtcgcgttcg aaatccgcga cccggtgatc cggctgtcgc ttgcgatctg
      241 gaacgccgtc agccgggcgg cgcggtggac ggcgggctcg ttcgtcttct gcgaggctgc
      301 cgcgttccgc agcctcggcg gcttcagcga ggcgctctac gccggcgaag agatcgactt
      361 cgcgcgccgc ttgaagcgcc ttgcgcgcag ccgcgggctc ggcgtcgtca tcctgcaccg
      421 ccatccgctg cgcacgagcg accgcaaggc ccgcctgtac accaagcgcg agcacttcgc
      481 gttcctgctg aagacgctcc tgctggcggg ccgcacgctg cgaccgcaac gcgtgctacg
      541 tctggtacga cggtagacgt tgagaattcg gggtcaggtc gcaattcgtt tttgcgtacg
      601 cgaaaactga attgcgaccc tgaccccgaa ttaggtctcg acgacgaagt cgaagctcgc
      661 ctcgtccgcc ttcgccttct tcatctcgag ctcggcccgg tagaggccgt cgcgcctgtt
      721 gcccgggtcg cccggaaagt agagctgcgt ggtcaggatg cgccttcccg gcgcctgcac
      781 cttgacgtgg atgtggcgcg tgcggccggg atactcggcg ggtacgatgg tctcgagccg
      841 gaagcgccct tgcgcatccg cgtgctggtg gccgcggtag cggaagccgc ggttgtcgta
      901 ctcgcccagc tcgtccgaat gccagaaatc gagcagcgcg ttcggcaccg ggttgcactg
      961 cgcggagagc accgtgccga tcacgaagat ccgggttttc tcgccgccct ccagcagtga
     1021 aacccgttgc ggcgatttcg gcgtgaagaa cggcccttcg gtctgccgcg gcgtgaggac
     1081 gccgcaggtc tgcgccagcc cctcgagcgg aagcagcagc gcggcgccca ggagctctct
     1141 tcgcctcatc ccttcacgca gatgacctgc ttgagggtgt gcacgatatc gaccaggtcc
     1201 gcctggtttt ccatcacctc gtcgatcggc ttgtaggcgc ccgggatctc gtcgagcacc
     1261 cccttgtcct tgcggcagat cacgccgtgc gtctggtctt cgaggtcctt cacggaaaac
     1321 ttcttctgcg ccgcgttgcg gctcatgagg cgcccggcgc cgtgggcgca cgagtcgaag
     1381 ctctcgggcg agcccttgcc gcggacaatg tagctgcgca cacccatgct gcccggaatg
     1441 atacccagct cgccagcgcc ggcgcggatc gcccccttgc gggtgagcca gacctgctcg
     1501 ccgtagtgct cctcgcgctc gacgtagttg tggtggcagt tgaccacgcc ctcggtcacc
     1561 tcggacgccg gcaggtggcg ctgcatcgcg ctcaggacga ggtccatcat ctcctcgcgg
     1621 ttcgcgcgcg cgtattcctg cgcccagccc acggcctcga tgtaatcgtc gaaatgcacc
     1681 gagccttcgc ggaaatacgc gaggtcgcgc tccgggagct gcacgttgtt gcgctgggcg
     1741 tccttcttgg ccagctcgat gaaatagctg ccgatggcgt tgccgatgcc gcggctgccc
     1801 gaatgcagca tcgcccacac ccggttctcc tcgtcgaggc agatctcgat gaagtggttg
     1861 ccgccgccga gcgtgccgag ctggttcacc cagctcgagt gcttgctgca gcgccgctcg
     1921 accccggggt gcttctcgaa gattttcgtc agttgcttct ggaaccgctt cgcggcatgc
     1981 gagcgcgcct cgcggtcgga gtgctggccg aatccggccg gcacgtcgcg gctgatctgc
     2041 gcgaagagct tcttcagcga ggcttcgtcg agctggtctg cgccgagcga aacgcgcgta
     2101 gccgtcatgc cgcagccgat gtcgactccc accgcggcgg gaatgatcgc cttccgcgtc
     2161 gggatcaccg atccaaccgt ggcgccgata ccgcggtgga cgtccggcat cgctgccaca
     2221 tggtggtgca cgatcggcaa gcgcgaaagg ctgacaagtt gctgacgcgc gccgggttcc
     2281 aggtcgcgcg tgtagacctt gaccggaacg agaccttcgg cgatttcctg ccggaccggc
     2341 atgtttctcc cttgttgtcc ggaaagacga acgctacgaa cggctcaaat catccgaatg
     2401 atggtagtcg cgggcctgct gaacaatgta aagaaatgtg tgagcaaagc gtgggcgctg
     2461 tcccacgctc acgctcgcgc tcagctattc agccgagcga atcatcgagg catagcggat
     2521 ttgaataccg aactactgcg ccaggcgaag gagcctcgcc gcgttttcct tcaggatgag
     2581 cggccgcacc tcgggcttga tcggcagctt gtcgaactcc tcgatccact tctccggcgt
     2641 gatcaccggg taatcggagc cgaaaagcac cttgtccttc agcagagtgt tggcgtactg
     2701 caccaggatc ggcggaaagt acttcggcga ccacccggac aaatcgataa atacctgcgg
     2761 cttgtgcgtt gccaccgaca gcgcctcttc ctgccaggga aaggaaggat gggcgagcac
     2821 gatcggcata tccggaaagt ccgccgccac gtcgtcgagg tatagcggat tcgaatactt
     2881 cagccgcagc ccaccgccgc cgcgcgtgcc tgcgcccatc ccggtctggc cggtatgaaa
     2941 gagcgccggc agcttcgcct ccgcgatgac ctcatataga ggataggcaa ggcggtcgtt
     3001 gggatagaac tcctgcaccg tgggatggaa cttgaagcct ttcaccttgt actcggcaat
     3061 caatcttttc gcggctcgca cgccgtcggc gccccggtgt gggttgatgc tggcgaacgc
     3121 aatggcgaca tcgccgttct tgatggcgag ctccgcgatc tcctcgttgg tgatctttct
     3181 gttgtcgcgg gggctatcga ccgtgaagac gacgaacccc atcttcattt tgcggtagta
     3241 gtccgccatc tccgggaccg tcgcgtgctg cggctggtag cggaagtagc gccccatcgc
     3301 ttcctgggac tcggtcagct tgtcgtcgac ctgcagcgcg gacttccacg cgtgggtgtg
     3361 gacgtcgatg gctacgagct ctttgacgtt catgtcagca ataccctggg cgaatccgta
     3421 tagagctcct ccaccagcgc tgcgcggcgc cgcagcaccg cgcgctggtt gatcgagccc
     3481 ttgtcggtca tctcgcccgc gtcgagagac ggcggctcct cgagcaccag cgcgcgcgcg
     3541 atccggtttg agctgcctgt actcgccagc gtcttgagtc ttttcaggag ctcatccgcc
     3601 ggcgcattgc cagccggaaa tatcaggacc ccgacctcgc tcttcccctc tccggcaatc
     3661 accacgtcgc gcaccagcgg cgcgaagtgg gcgatgaaaa gcgcgcgcag cgggccgacg
     3721 ttgacccagg tgccgctcga gagcttgaaa tcctccgcga tgcggccacg gaaaagcagg
     3781 ccttcgaccg gcttttgttc atttgccaag acaaaggagt cgccgaggcg gtaatagcct
     3841 tcctcgtcga acacctgcgc cgtgagctcg ggctgtcgcc agtagccggg cgtgatgttc
     3901 gggcccttga gccgcgcctc gagcttgtcc tgcaacggga ccagcttgat ctcgaccccc
     3961 ggcggcggca ggcccatgtt ggtgacgtct ttcgtttccc acatgcgtcc catcgcgaac
     4021 ggcgcggtct cggtcgagcc caggccggtg aggaacagga tctcctcgcc gcaagtctca
     4081 taggcgagcg ccttgacctc gtcgaacacc ggctgcgaca cggcggcgcc ggcgaaccac
     4141 agcagcttga gccggctgaa gaagttcttt cttagtccaa gatcagtcct taggaaaggc
     4201 agcagcacct cgtagcccct cggcacgttg aagtaccagg ttggcgcgat ttcgcgaagg
     4261 ttgcgcaccg tcgtctcgat cgcgccgggc agcggcttgc cctcgtcgat gtagtacgag
     4321 ccgccgttgt agagcaccag cccgaaatcg tggttgcccg ccgcggtgtg gtgccacggc
     4381 gcccaatcga cgaccagcgg cggctcctcc tcgaagaacg cgaacatgaa gcggatcatt
     4441 tcctggttcg agcaccacat gcgctgcgtg ttgatcaccg ccttcggctc gccggtggag
     4501 ccggaagtga acaggaattt cgcgatcgtg tccgggccga cccgggcatg tgcctcgtcc
     4561 actgcgccgg tcgcttcgcc ggcctccagc ttctggactt cataaaccac ctcggtgccg
     4621 cccggcacgg cggcggcgat cgccgcctgg aacggttcgc gcaccgacgc gaacaccagc
     4681 cccggcgtga gcaagcgcat gacgtgcttc agcttggcga agtccttcga cagcagcgaa
     4741 tacgccggcg acaccggcgc atagggcacg ccggcgtaca gcgcgccgag ttcgatcagc
     4801 gcgtgcgcga agtcgttgcc ggagagcacc gccaccggac gctctgcgga gagccgccgc
     4861 tcgagcagcg cctgcgcgat gcgccgggcg cggctgcgcg cctgcgcata ggtgagccgc
     4921 tgccacacgc cgtccggtcc gcgctgggcg aggaacacgc gctccggcgc gtgctctgcc
     4981 caatggtcga gccgctcggt gagcttgcgc ggatacgccg ggagcggatc cggcgagcga
     5041 aggtagacgt tccccgcgcg ccgctcggcg agcaccgtct ggcgggccag ccggacgctg
     5101 cgcgtccggc gcgcgtgctg cggctggctc aagactcggc ggtaaagcct gtctgcttga
     5161 cgagaccacc ccactctgcg gtgtccgcct tgaatacctc ggcgagctgc gccggactcg
     5221 agccctgtac ctccaggccg aactgcttcg cgaactccac cacttcggcc tgcccgagca
     5281 ccgcctgcag cgcggcggaa gcgcgctgca cggtctcggc gctcgcgcgc gccggcatga
     5341 agaacgcgta ccactcgcgc agcgcgatct tgaagccctg ttcgcccatg gtcggcaccg
     5401 cgggcaggaa ggcgctgcgc tcctttccgg aggtgccgag cacgcgcacc ttgctggcct
     5461 gcgagtgcgc gaggacatcg cccgtcggac cgaggaagga ggcgatctgc ccgccgagga
     5521 ggtcctggat ccccggcgcg cttccgcgat accccgcgtg gcgcaggttc gcgccggccg
     5581 ctttgtcgag cagcacgccg atgagatgcg gcatcgagcc cgcgcccggg ctgccgtaga
     5641 ccgccttgtc cgggttggcc ttggcccagg cgaaaaagtc cttgagcgtc ttcacgctgt
     5701 cgggcaccgc gggcccgacg gagagcacgt ggtggctgta catcgcgagc gacaccggtg
     5761 ccacatcctc gacccggtac tgcagcttcg ggtacgtgtt cgggtagatc gtgagcatcg
     5821 acgaaggcgt gaggagcagc gtggcgccgt cgggcggggc atccttcagc gcgacgacgc
     5881 cgagctgccc gcctgctccc ggacggttct cgacgatcgc ggtgcgcgcg taggcgccgc
     5941 gcagcttgtc cgcgaccttg cgcgccatga cgtcggtcgt cccgccgggg ggaaagccga
     6001 cgatgatctt cgcggtgtcg atgccctgcc cgcggacagc aaagggaagc gctccgccga
     6061 gaacgccaag cgcggcggtg ccgaggaacc tgcgacgtgt ctgcatgcga cctcctagct
     6121 caggacgacc tttctggatt ccgcgcgccg gcccggcgcc tccaggctgc gccgcaggat
     6181 cttctgcacc agctgatgtg ccaactcccg gggcgacttg cgccccgacc ggtaccagtt
     6241 gtgcgtccag ttcagcgcgc ccagcaggtt gaggcgcagc aaggtctggt cgacgtcggg
     6301 cgggagttcg agcgcggcga tcatctggcg aaagcgctcc tcgtagacgt cgcgatcgcg
     6361 gttgaggcgc cgctgcagcg gcgagcgcgc aggatggaac aggctcgtgc ctccgaccag
     6421 ggactcgtac tcctcgccta ccagcaaggt taggtgcgcg gcgcacgcgg cttcgagcct
     6481 cgcccagg
//
LOCUS       DATBSZ010000149        13850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2139954, whole genome shotgun sequence.
ACCESSION   DATBSZ010000149 DATBSZ010000000
VERSION     DATBSZ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13850
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139954"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VIG70_09905"
     CDS             complement(<1..1178)
                     /locus_tag="VIG70_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY8250910.1"
                     /translation="MNQKVLSKDQDPAVTAAAPAKQFALKPLPEDALIVIPMRNTVLF
                     PGVISPITVGRVASVAAAHEAVRTEKKVGFLLQRDPQTNDVQPSDLYWVGTAGQVVRY
                     ITGAEGTHHLVVQGQSRFRVLEFLHGWPFLVARVQLIEQREEANPDIEARFLQLKSQA
                     VEAIQLLPSAPEELTVVVQGMTSPALLADMVANLLDIKNEDKQDLLETFDLKRRLDKV
                     LEHLGERVGVLRISKEIGDKTKKQFDERQREHVLREQMRQIQKELGEGEDSAAELEEL
                     KKGIEDAGMPEDVHKHAVKELKRLQRMGEGSAEGSMLRTYLEWLAELPWKVSETKEIN
                     ISEARKVLDEDHFGLDKIKRRILEHLAVRKLNPEGKSPILCFVGPPGVGKTSLGQSIA
                     R"
     gene            complement(1175..1585)
                     /locus_tag="VIG70_09910"
     CDS             complement(1175..1585)
                     /locus_tag="VIG70_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY8250911.1"
                     /translation="MRSRDFSSWMWGEALSMLDRAQRLQRQFFTHAAVAWEPPVDIVE
                     TGETLQMHVALPGVSADTVTVALDPAGITISAVRPFPCAGETSRIHRLEIPYGRFERH
                     IGLQLGDPYSPLELASRSLRDGVLTLTFRRKEGA"
     gene            complement(1642..1911)
                     /gene="infA"
                     /locus_tag="VIG70_09915"
     CDS             complement(1642..1911)
                     /gene="infA"
                     /locus_tag="VIG70_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY8250912.1"
                     /translation="MAKEEMLEMDGTVQEVLPNTNFRVELENGATVLAYAGGRVRQNR
                     IRILAGDRVRLEISPYDVTRGRINYRHKDERPVATPQRRPTWKKR"
     gene            complement(1991..2929)
                     /gene="gcvA"
                     /locus_tag="VIG70_09920"
     CDS             complement(1991..2929)
                     /gene="gcvA"
                     /locus_tag="VIG70_09920"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEY8250913.1"
                     /translation="MIYRLPPLNALRVFEAAARHLSFKEAAVELHITQAAVSHQVKSL
                     EEYLGVQLFRRTARGVQLTDAARACLPKLREGFDALAGAVEMIRERGDEAQLVITAPP
                     VFTARWLMPRLADFSKREPKIELRVFASSKMVDAGALDSTSLVGDLDLRTESSAVQIH
                     LGAGQYPGHRADRLFGVSMAAVANPGLVKESGLKVPADLASHTLLHDDAMEIVAGGNA
                     WRKWLEAAGVADRVDADRGPRFSSNILSLEAASQKLGIALALRPLVDADIASERLCAP
                     FEVEVKPQSAYYLVCPEVIADRPAVAAFRKWLLAQV"
     gene            3154..3405
                     /locus_tag="VIG70_09925"
     CDS             3154..3405
                     /locus_tag="VIG70_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250914.1"
                     /translation="MRKDFAMHREFSTAFWQRAYDSLPRQVRGQYLTQIKAAEQWELA
                     LGDLIKVWSGAKNTVSSFFKNYWLPRRPSKSEASSSSSG"
     gene            3443..3880
                     /locus_tag="VIG70_09930"
     CDS             3443..3880
                     /locus_tag="VIG70_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY8250915.1"
                     /translation="MKPAPRAANAVARAAGAVVFRRSERGIRVLVLRAYKNWDFPKGR
                     VEPGESELDAARREVAEETGLAGLDYPFGEEFRETVPYAGNKVARYYLAETDAEKIEL
                     PVSPELGRPEHHEYRWVSFDEAEDLLPPRLAVVLEWAKKTIAA"
     gene            3902..4240
                     /locus_tag="VIG70_09935"
     CDS             3902..4240
                     /locus_tag="VIG70_09935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY8250916.1"
                     /translation="MDKLIIEDLVVGTGAAPTAGQKVTVHYTGWLMEGGKAGAKFDSS
                     KDRRDAFVFQLGKGQVIKGWDEGVQGMKVGGKRKLTIPPALGYGSRGAGGVIPPNATL
                     VFEVELLGVG"
     gene            complement(4310..7030)
                     /gene="ppc"
                     /locus_tag="VIG70_09940"
     CDS             complement(4310..7030)
                     /gene="ppc"
                     /locus_tag="VIG70_09940"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY8250917.1"
                     /translation="MRADDTLPFVEEKELLLREDRRLLGRLLGEVIREQVGEEALERI
                     ERIRQTAVKFRREEAGNEGSSRGELERELNSLNLQQTLHVVRAFSYFSHLLNIAEDEQ
                     QHRRRRAHAAAGSPRRAGSFSYALDRAREAGVAAPALVTWFDRARVAPVLTAHPTEVQ
                     RQSILDCEREIARLLSRDDSPERTEALHAEILRLWLTAMLRGTRLEVSDEVANGLAYF
                     RLTFLEELPHLYAEFERALKEKYDIEARLAPFLTVGTWIGGDRDGNPYVNAAVMRGAL
                     AQQAGLILTHYLEEVGRLYKELALSARIHAAPAGIEALAAASGESSAYRRDEPYRRAI
                     SGIYARLAGAAENLAGVKANPKAAVIQPAYGSAEEFSRDLQTVSQSLESQGAGRLARG
                     RLAALQRKVSLFGFHLAPIDLRQSAQEHARTVAELLARAGVASDYAAMDENGRVAVLA
                     KELSGPRPLRVPHLEYSQFVENELAILAAAAEGKRRFGARAVPHYVISHCSAVSDLLE
                     VGVLLREVGLLEQVDIIPLFESIADLGRCGDVLADALDLPLYRGWVDARGGQQEVMLG
                     YSDSNKDGGYFTSNWSLYKAGSTLVEVCRKRGVRLRLFHGRGGTIGRGGGPSYEAVLA
                     QPAGSVDGALRLTEQGEVIASKYADPESGRRNLETLAAATLEASLKNETHEDRRYAAI
                     AEALSARAFEAYRGLVKTPGFVEYWRLSTPISEIADLNIGSRPASRPGSNAAHERIED
                     LRAIPWVFSWSQCRLMLPGWYGFGSAVEGWKGSVEELREMHREWPFFRSVLSNLDMVL
                     AKTDLAIASRYAELVLDKRLRREIFEKISDEWSRTRKWLAAITGNAELLVDNPTLARS
                     IRSRFPYLDPLNHVQVELLRRYRAGGRDEQLLRLIHLTINGLAAGLRNSG"
     gene            7029..7214
                     /locus_tag="VIG70_09945"
     CDS             7029..7214
                     /locus_tag="VIG70_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250918.1"
                     /translation="MGKLLVVIAIIAAVAYAWHQGLIQRWIGSAVDSGIENTRRIQRD
                     ATKVRPADPGAPEQEKK"
     gene            7332..8840
                     /gene="gshA"
                     /locus_tag="VIG70_09950"
     CDS             7332..8840
                     /gene="gshA"
                     /locus_tag="VIG70_09950"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY8250919.1"
                     /translation="MRALPAGALRDLRRGIEKESLRVRPDGSLALTPHPARLGSALTH
                     PHITTDFSESQLELITGVHPDADACLQELTEIHEVVIRAIGEELLWASSMPCNLPADE
                     AIPIGRYGTSNVGRAKTVYRNGLSYRYGRRMQAISGIHYNFSLPGIGSDGYFALIRNF
                     RRLSWLPLYLFGASPAVCSSFVEGRAHQLQELSSGTHYLPHATSLRMGRLGYQSDAQA
                     SLAVSYNNLTSYATSLHEALTKPYPPYEKIGIRDGDAYRQLSTSLLQIENEFYSPIRP
                     KRRIKSGERPLHALRERGVEYVEVRLMDLDPFNPVGINAQTMRFLDILLLYCLLEDSP
                     PDSPQELKAIARNKLRGAQEGRKPGLLLERRSEQVTLAGWGKEVLSACEPISVALDEA
                     RRGAAYRDALESAMNSLDNPEAVPSARVLHAMARNHANSHVRFTLAESLSHRGTLLGI
                     PLKPQVEERFARLANESIQKQRKIESLDRIDFETYRQRYLNPELLRAEALEK"
     gene            complement(8911..10044)
                     /locus_tag="VIG70_09955"
     CDS             complement(8911..10044)
                     /locus_tag="VIG70_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8250920.1"
                     /translation="MFAWLFLWMAGAALADEGSFGPRLVQEEVRIPAGRYTIAATILR
                     PQGDGPFGAVVLNHGTPGSASGRANESADLLIHSAAVFARRGYVVVLPLRRGFGATGG
                     DFAEDPGTCANPDYRKGEHAASDDVMAAYEYARALPYVDGNRMILAGQSAGGIVSMYA
                     AGTRNPQGLVAVLSFAAGRGGNPDFRPGVPCAVEPVARLLESVGKNVKAPVLLHYAEN
                     DLYFNAQTSRLWYERFAASGARAEYVLQPAFGRDGHYIFSETLGVRYWLPAVEQFLGK
                     HNVPFERLDAAQPLQAVERLPHVRTDACRNLYRAFLESPGPRAYAVSGDGRCGFAGGL
                     PDAADVAVRECRTNAKEPCSLYAVDAELVWAPDGKGTAYAGGK"
     gene            10159..10974
                     /locus_tag="VIG70_09960"
     CDS             10159..10974
                     /locus_tag="VIG70_09960"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEY8250921.1"
                     /translation="MKIRVKNVAPARQGKLAVTADLRSLLRRSGQTLVAPGASDALSA
                     RLIEAHGYECVYIGSYATAASRFGLPDTGRLSLEQLAAQAKTIVDAVRIPVIADAEGF
                     DNAVPAFEAAGVGAIHIEDHTGAGKHTSAPQKLHPMKEAAERIRQAVAARKNPDFLIV
                     ARSDAFWVHRDLEDCIARLELYASAGADMVFPTMVGPGELAEVRRRVARPLMIVDNPE
                     EQHEGAAIVLYYAFSITRQFAALQQALATRKLASDTAPLEDFLGYKDAPGRNR"
     gene            10971..11921
                     /locus_tag="VIG70_09965"
     CDS             10971..11921
                     /locus_tag="VIG70_09965"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8250922.1"
                     /translation="MRFFLAVLLLASGSAFAQGSYPNKPIRLIVGFPPGGSADPTTRI
                     IGAALQEQLGQPVVVENRPGADSAIAAEQMTRMPPDGYTLMFASNSAMTAAVALRKAP
                     TYDPLKDFTPVSMVGRATVFFYVHPSIPAKTLKEFIAHAKANPGKVVYGTGNPLSILY
                     GQQLTSATGIDWLHVPYKGEGPLNPDILAGRVHCSFLSTGSAVSNAKDGRLRPLAVLA
                     EKRSALLPDVPTISEAGVPEVTVRQWAGVYGPPKMPREIVERLNKEVNAALKRPDVLE
                     KLQSYGYAAEGSTPERLLEINRADLELWRKLVKEAGIALE"
     gene            complement(11857..12378)
                     /locus_tag="VIG70_09970"
     CDS             complement(11857..12378)
                     /locus_tag="VIG70_09970"
                     /inference="COORDINATES: protein motif:HMM:NF019416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEY8250923.1"
                     /translation="MTEAGAVFSPCRRWRYLLWRCWDESKPAANFLMLNPSTADEFKL
                     DPSCTRARNYAERWGFGAVVITNIFAWRSTDPAVLRVVKNPVGRGNDAAILRAAKEAK
                     LVVCAWGEHGEHRGRGAEVREILRKKKIPLHFLRLNGSGHPAHPLYLPGTLTPALFPP
                     PSRACATARDRRG"
     gene            complement(12375..12674)
                     /locus_tag="VIG70_09975"
                     /pseudo
     CDS             complement(12375..12674)
                     /locus_tag="VIG70_09975"
                     /inference="COORDINATES: protein motif:HMM:NF008448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
     gene            complement(13182..>13850)
                     /locus_tag="VIG70_09980"
     CDS             complement(13182..>13850)
                     /locus_tag="VIG70_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY8250924.1"
                     /translation="GFSWKPLKTGSAGLDELMLYLGEAPWEGQVQRVREWYLPHLERI
                     YDAAQVRAGDLLQLEKISTQFNERSVFLTEMALDPPSATSDLSGEPYLDEDYLVLSTV
                     HSAKGQEWEAVHILNVADGNFPSEFSTGRPELIDEERRLLYVAMTRAKSELHVLAPLK
                     YYVASQSRMGDRHVYGAKSRFMTKAVLACMEPLAWGEIAQSEETTAADYKVDVAARLR
                     GMWT"
BASE COUNT         2298 a         4726 c         4526 g         2300 t
ORIGIN      
        1 cgcgcgatcg actggccgag cgaggtcttg ccgacccccg gcgggccgac gaagcaaagg
       61 atggggctct tgccctcggg gttgagcttg cgcaccgcga ggtgctcgag gatccggcgc
      121 ttgatcttgt cgaggccgaa gtggtcctcg tccaggacct tccgcgcttc cgagatgttg
      181 atttcttttg tttcagaaac cttccaggga agctcggcaa gccattccag gtaagtccgc
      241 agcatcgagc cctcggcgct gccctcgccc atgcgctgca ggcgcttgag ctccttgacc
      301 gcgtgcttgt gcacgtcctc gggcatgccg gcgtcctcga tgcccttctt cagctcctcg
      361 agctcggcgg cgctgtcctc gccctcgccg agctccttct ggatctggcg catctgctcg
      421 cgcagcacat gctctctttg tctctcgtcg aactgcttct tggtcttgtc gccgatctcc
      481 ttcgagatgc gcagcacgcc cacgcgctcg cccaggtgct cgagcacctt gtcgagccgg
      541 cgcttcaggt cgaaggtctc gagcaggtcc tgcttgtcct cgttcttgat gtcgagcagg
      601 ttcgcgacca tgtcggcgag cagcgccggc gaggtcatcc cctgcaccac gacggtgagc
      661 tcctcgggcg cgctcggcag gagctggatc gcctcgaccg cctggctctt cagctgcagg
      721 aagcgcgcct cgatgtcggg gttcgcttcc tcgcgctgct cgatgagctg gacgcgcgcc
      781 accaggaacg gccagccgtg caggaactcg agcacgcgga agcggctctg cccctgcacc
      841 accaggtggt gcgtgccctc ggcgccggtg atgtagcgca ccacctggcc ggcggttccg
      901 acccaataga gatcggaggg ctgcacgtcg ttggtctggg ggtcgcgctg caggaggaag
      961 ccgaccttct tctcggtgcg caccgcttcg tgcgcggcgg cgaccgacgc gacgcgtccc
     1021 acggtgatcg gcgagatcac gcccgggaac agcaccgtgt tgcgcatcgg gatgacgatg
     1081 agcgcgtctt ccggcagcgg cttgagagca aattgctttg ccggggccgc ggcggtgacg
     1141 gccgggtcct ggtccttgga aagaactttc tggttcatgc gccttccttc cggcggaacg
     1201 tcaacgtcag gacgccgtcg cgcagcgacc tggaggcgag ctccagcggc gaataagggt
     1261 cgccgagctg cagcccgatg tggcgctcga accggccgta ggggatctcg aggcgatgga
     1321 tccgggaggt ttcgccggcg cacggaaacg ggcgcacggc ggaaatggtg atgcccgcag
     1381 ggtcgagcgc cacggtgacg gtgtcggcgc tcacgccggg gagcgccacg tgcatctgca
     1441 gcgtctcgcc ggtctcgacg atgtccacgg gcggctccca cgccacggcg gcgtgggtga
     1501 agaactggcg ctgcaggcgc tgcgcccggt cgagcatcga cagggcttcg ccccacatcc
     1561 agcttgaaaa atctcgcgat ctcaccgccg ttagatggcg gcgcttaccc cgggttcaag
     1621 tgcgggctaa accccgcttt cctagcgctt tttccaggtg ggccgccgct ggggcgtcgc
     1681 gacgggcctt tcgtccttgt ggcggtagtt gatgcgcccg cgggtgacgt cgtagggcga
     1741 aatctcgagc cgcacccggt cgccggcgag gatgcggatg cggttctgcc gcacccggcc
     1801 gccggcgtag gcgagcaccg tcgcgccgtt ctcgagctcg acgcggaaat tggtgttggg
     1861 caggacttcc tggaccgtgc cgtccatctc gagcatctct tccttggcca tgacaaaacc
     1921 tctcgtgcgc cgtggcggcg cgtgggcggt gcggtggtaa ctgcagcggg caggaaaact
     1981 acaggtgcaa ttatacctgc gccaggagcc acttccggaa agcggcgacc gcggggcgat
     2041 cggcaatcac ctccgggcag acgaggtaat aggcagactg cggcttcact tcgacctcga
     2101 agggcgcgca cagccgctcg gaggcgatat ccgcatccac cagcgggcgc agggcgagcg
     2161 cgatgccgag cttctgcgac gcggcctcga gcgacaggat gttcgaggag aagcgcggac
     2221 cgcggtcggc atcgacacgg tcggcgacgc cggctgcctc gagccatttg cgccacgcgt
     2281 tgccgccggc gacgatctcc atggcgtcgt catggagaag cgtgtggctc gccaggtccg
     2341 ccggcacttt caatccgctt tccttgacca ggcccgggtt cgcgaccgca gccatcgaga
     2401 cgccgaacag gcgatccgcg cgatggcccg gatactggcc ggcgccgagg tggatctgga
     2461 ccgcggaaga ctcggtgcgc aagtcgagat cgcccacgag cgaggtggag tcgagcgccc
     2521 ccgcgtccac catcttgctc gaggcgaaca cgcgcagctc gatcttcggc tcgcgcttgg
     2581 agaagtcggc gaggcgcggc atcagccagc gggcagtaaa gaccggcggc gcggtgatga
     2641 cgagctgagc ttcgtcgccg cgctcgcgga tcatctccac ggcgccggcg agggcatcga
     2701 agccttcgcg cagcttgggc aggcaggcgc gcgccgcgtc ggtgagctgc acgccgcgcg
     2761 ccgtgcggcg gaacaattgc acaccgaggt attcctcgag gctcttcacc tggtggctca
     2821 ccgcggcctg cgtgatgtgc agctccaccg ccgcttcctt gaacgacagg tggcgcgccg
     2881 cggcttcgaa tacccgcagc gcattcaagg gcggcaggcg ataaatcaca ggatggatga
     2941 gtttttctta tgcaaggggt gagatttcat cgtttgtgca tgcagcgccc cgtccctata
     3001 ttcactgcat gaaaaaccgt tatccgactg cgaaagaatt gtacgcgctc gagcacgaag
     3061 ctcgtcgctt gcgcgcggcc gagatggcgc ggctcgtccg cgctggagcc aaggcggtgc
     3121 gcgcgctttt cgcgcgcatt gttacggttc gcaatgcgaa aggacttcgc catgcatagg
     3181 gaattcagca ctgctttctg gcaacgcgcc tatgactcgt tgccgcggca agtacgcggg
     3241 caatatctca cgcagatcaa ggctgcggaa cagtgggaac tcgcgctcgg cgacctgatc
     3301 aaagtctggt cgggcgcaaa aaacacggtt tccagctttt tcaagaatta ctggctgcct
     3361 cgccgtccga gcaaatccga agcctcctcc tcctcctctg gctagggccc gctcgggcgg
     3421 cgagcatcta taaaatggcg gggtgaaacc cgccccccgc gccgcaaacg cggtcgctcg
     3481 cgccgcaggc gcggtcgtat ttcggcgcag cgaacgcggc atccgcgtcc tcgtgctgcg
     3541 cgcctacaag aactgggact tccccaaggg cagggtcgag cccggcgaga gcgagctcga
     3601 cgcggcgcgg cgcgaagtcg ccgaggaaac cgggctcgcc ggcctggact atcccttcgg
     3661 cgaggagttc agggaaaccg ttccctacgc cggcaacaag gtcgcgcgct attacctcgc
     3721 cgagaccgac gccgaaaaga tcgagctccc cgtttcgccc gagctcggcc gccccgagca
     3781 ccatgagtac cgctgggtaa gcttcgacga ggccgaggac ctcctgccgc cacgcctcgc
     3841 cgtcgtgctc gaatgggcga agaaaaccat tgcagcctga atttcatccc ccggagaaaa
     3901 catggacaag ctgatcatcg aagacctcgt cgtcggcacc ggcgccgctc ccaccgccgg
     3961 ccagaaagtg accgtgcact acaccggctg gctcatggag ggaggaaaag cgggcgccaa
     4021 gttcgattcg agcaaggacc gccgcgacgc cttcgtgttt cagctcggca aaggccaggt
     4081 gatcaagggc tgggacgaag gcgtgcaggg catgaaagtc ggcggcaagc gcaagctcac
     4141 catcccgccc gccctgggct acggctcgcg cggcgccggc ggcgtgatcc cgccgaatgc
     4201 gacgctggtg ttcgaggtcg agttgctcgg agtgggataa gaattcgggg tcagggtcgc
     4261 aattcagttt tcacgtacgt aaaaacgaat tgcgaccctg accccgaatt catccgctgt
     4321 tgcgcaggcc cgctgccaat ccgttgatgg tcaggtgaat cagccggagt agctgctcgt
     4381 cgcggccgcc ggcgcggtag cgccgcagga gctcgacctg cacgtggttg agcggatcga
     4441 ggtagggaaa gcggctgcgg atcgagcgcg cgagagtggg gttgtcgacg aggagctcgg
     4501 cgttgccggt gatcgcggcg agccacttgc gcgtgcggct ccattcgtcg gagatcttct
     4561 cgaagatctc acggcggagt cttttgtcca aaaccaattc tgcgtaacgc gaagcgatgg
     4621 cgaggtcggt cttggcgagc accatgtcga ggttcgacag cacgctcctg aagaacggcc
     4681 actcgcggtg catttcccgc aattcttcaa ctgaaccctt ccaaccttct accgcgctgc
     4741 cgaagccgta ccagcccggc agcatcagcc ggcactggct ccagctgaac acccagggaa
     4801 tcgcgcgcag gtcctcgatc cgctcgtgcg cggcgttgct tcccgggcgg cttgccggac
     4861 ggctgccgat gttcagatcg gcgatctcgg agatcggcgt cgacaggcgc cagtactcga
     4921 cgaagcccgg cgtcttcacc aggccgcggt aggcctcgaa cgcgcgcgcg gaaagcgcct
     4981 ccgcgatcgc cgcatatctg cggtcttcgt gggtttcatt tttcaggctg gcttccaggg
     5041 tcgcggccgc gagggtctcg aggttgcgcc gcccgctctc cgggtcggcg tacttgctcg
     5101 cgatcacctc gccctgctcg gtaagccgca gcgcgccgtc gaccgagccc gccggctgcg
     5161 cgagcaccgc ctcgtagctc ggcccgccgc cgcgcccgat cgtgccgccg cggccgtgga
     5221 agagccgcag ccgcacgccg cgcttgcggc acacctcgac cagcgtcgac ccggccttgt
     5281 acagcgacca gttcgaggta aagtagccgc cgtccttgtt gctgtcggag tagccgagca
     5341 tcacttcctg ctggccgccg cgagcgtcga cccagcccct gtagagaggc aggtcgagcg
     5401 catcggcgag gacgtccccg cagcgtccca ggtcggcgat cgactcgaac agcggaatga
     5461 tgtccacctg ctcgagcagc ccgacctcgc gcagcagcac gccgacctcg agcaggtccg
     5521 acaccgccga gcagtgcgag atcacgtagt gcggcaccgc gcgcgcgccg aagcgccgct
     5581 ttccctcggc cgccgcggcg aggatggcga gctcgttctc gacgaactgc gaatactcca
     5641 ggtgcggcac ccgcagcggc cgcggcccgg agagctcctt cgcgagcacc gccacgcgcc
     5701 cgttctcgtc catcgccgcg tagtcggaag cgacgccggc gcgcgccagg agctccgcca
     5761 ccgtgcgcgc gtgctcctgc gcgctctgcc gcaggtcgat cggcgcgaga tggaagccga
     5821 acaggctcac ctttcgctgg agcgccgcca ggcgtccgcg cgccagccgg ccggcgccct
     5881 ggctttccag cgattgtgaa accgtctgca gatccctgga aaattcttcc gcactcccat
     5941 aagcgggctg tattacggcc gccttcggat tcgccttcac cccggcaaga ttctccgcgg
     6001 cgccggcgag ccgcgcgtag atgcccgaga tcgcgcggcg gtacggctcg tcgcggcggt
     6061 atgccgagct ctcgcccgaa gccgcggcga gcgcctcgat cccggcgggc gcggcgtgga
     6121 tccgggccga gagcgcgagc tccttgtaga gccggccgac ttcctcgaga tagtgcgtga
     6181 ggatgagccc cgcctgctgg gccagcgcgc cgcgcatgac cgcggcgttc acataggggt
     6241 tgccgtcgcg gtcgccgccg atccaggtgc cgacggtgag gaaaggcgcc agccgcgctt
     6301 cgatgtcgta tttctccttc aatgccctct cgaactcggc atagaggtgc ggcagctcct
     6361 ccagaaacgt caaacggaag tacgcgaggc cattcgccac ttcgtccgac acctccaggc
     6421 gcgtgccgcg cagcatcgcc gtgagccaga gccgcaggat ttcggcgtgc aaggcctccg
     6481 tccgctcggg cgaatcgtcc ctggacagga ggcgcgcgat ttcgcgctcg cagtcgagga
     6541 tgctctggcg ctgcacctcg gtgggatgcg cggtgagcac cggcgccacg cgcgcgcggt
     6601 cgaaccacgt gacgagcgcg ggcgccgcga cgccggcctc gcgcgcgcgg tcgagcgcat
     6661 aggagaagct gcccgcgcgc cgcggcgagc cggccgcggc gtgcgcgcgg cgccggcggt
     6721 gctgctgctc gtcctcggcg atgttgagga gatgcgagaa gtagctgaaa gcccgcacga
     6781 catgcagcgt ctgctgcagg ttgagggaat tcagttcccg ctcgagctcg ccccggctgc
     6841 tgccttcatt tcccgcttcc tcgcgccgga acttcaccgc ggtctgccgg atgcgctcga
     6901 tgcgctcgag cgcctcctcg cccacctgct cgcggatcac ttcgccgagg agccgcccga
     6961 ggagccggcg gtcctcgcgc agcagcagtt ccttctcttc cacgaatggc agcgtatcat
     7021 cggcccgcat gggaaaactc ctcgtcgtga tcgccatcat cgcggccgtc gcgtacgcgt
     7081 ggcaccaggg cttgatccag cgatggatcg gctcggcggt cgacagcggc atcgagaaca
     7141 cgcggcgcat ccagcgcgac gcgaccaagg tgcggcccgc cgacccgggt gcgcccgagc
     7201 aggaaaaaaa gtagtaggat cgtccggcga ggacaaggag gttcaaccat gtgttacgac
     7261 tacgagcccg agtacctgcc ggtcgaagtc cctaaagaag ccccggtttc cgcctgagcc
     7321 tcgcggagcg cttgcgcgcc cttcccgcgg gcgcgctgcg cgatttgcgc cgcgggatcg
     7381 agaaggagag cctgcgcgtg cggcccgacg ggtcgctcgc gctcaccccg cacccggcgc
     7441 gcctcggctc ggccctcacc cacccgcaca tcaccaccga tttcagcgag agccagctcg
     7501 agctgatcac cggcgtccat cccgacgccg acgcctgcct gcaggagctg accgagatcc
     7561 acgaggtggt gatccgcgcg atcggcgagg agctgctttg ggcgtcgagc atgccctgca
     7621 acctgcccgc cgacgaggcg atcccgatcg ggcgctacgg cacctcgaac gtcggccgcg
     7681 ccaaaaccgt gtaccggaac ggcctctcgt accgttacgg ccggcgcatg caggcgatct
     7741 cgggcatcca ctacaacttc tcgctgcccg gcatcggcag cgacggctac ttcgcgctga
     7801 tccgcaactt ccgccggctg tcctggctcc cgctttatct tttcggcgct tcgcccgcgg
     7861 tctgctcgag cttcgtcgaa ggccgtgcgc accagcttca ggagctctct tccggcacgc
     7921 actaccttcc gcacgcaacc tcgctgcgca tgggacgcct gggctaccag agcgacgcgc
     7981 aggcgagcct ggccgtgagc tacaacaacc tcaccagcta cgcgacttcc ctccacgagg
     8041 cactcaccaa gccgtacccg ccctacgaga aaatcggcat ccgggacggg gatgcctatc
     8101 ggcagctctc cacgagcctg ctgcagatcg agaacgagtt ctacagcccg atccggccca
     8161 agcgccgcat caagtccggc gagcggccgc tgcacgcgct gcgcgagcgc ggcgtggagt
     8221 acgtcgaagt ccgcctgatg gacctcgacc ccttcaatcc ggtcggcatc aacgcgcaga
     8281 ccatgcgctt cctcgacatc ctgctgctct actgcctgct cgaggacagc ccccccgatt
     8341 ccccgcagga gctcaaggcg atcgcgcgca acaagctgcg cggcgctcag gagggccgca
     8401 agcccggcct gctgctcgag cgccgcagcg agcaggtcac gctcgccggc tggggaaaag
     8461 aggtgctgag cgcgtgcgaa ccgatttccg tcgcgctcga cgaagcgcgc cgcggggcgg
     8521 cttatcgaga tgccctggaa tcggccatga attctctaga caacccggag gccgttcctt
     8581 ccgctcgcgt gctgcacgcc atggcgcgca accacgccaa ttcgcacgtc cggttcacgc
     8641 tcgccgagtc gctgtcgcac cgcggcacgc tgctcggcat cccgctgaag ccccaggtcg
     8701 aggagcgctt cgcgcgctta gcgaacgagt cgatccagaa gcagcgcaag atcgaatcgc
     8761 tggatcggat cgacttcgag acctaccgcc agcgctacct caacccggaa ttgctccgcg
     8821 ccgaagccct agagaaataa ttcggggtca gggtcgcaac tcgtttttgc gtacgcgaaa
     8881 acgagttgcg accctgaccc cgaattacgc ttacttccct ccggcgtacg cagtcccctt
     8941 gccgtcgggc gcccacacca gctcggcgtc gaccgcgtag agggagcagg gctccttggc
     9001 gttggtgcgg cactcgcgca ccgcgacgtc tgcggcgtcg ggcaggccgc ctgcgaaacc
     9061 gcagcggccg tcgccgctca cggcgtaggc gcgcggcccg ggcgactcga ggaaggcgcg
     9121 gtagaggttg cggcaggcgt cggtgcgcac gtgcggcagg cgctccaccg cctggagcgg
     9181 ctgcgccgca tcgaggcgct cgaacgggac gttgtgcttg ccgaggaact gctcgaccgc
     9241 gggcagccag tagcgcacgc cgagcgtctc gctgaagatg tagtggccgt cgcggccgaa
     9301 cgccggctgc agcacgtact cggcgcgcgc gccgctcgcc gcgaagcgtt cgtaccacag
     9361 gcgcgaggtc tgcgcgttga agtagaggtc gttctcggcg tagtgcagca gcaccggcgc
     9421 cttcacgttc ttgccgaccg actcgagcag ccgcgcgacc ggctcgaccg cgcacggcac
     9481 gccgggccgg aaatcgggat tgccgccgcg ccccgcggcg aagctgagca cggcgaccag
     9541 cccctgggga ttgcgcgtgc cggcggcgta catcgacacg atgccgcccg ccgactggcc
     9601 ggcgaggatc atgcgattgc cgtcgacgta aggcagcgcg cgcgcgtact cgtaggccgc
     9661 catcacgtca tcggacgcgg catgctcgcc cttgcggtag tccggattcg cgcacgtgcc
     9721 ggggtcctcg gcgaaatcgc cgccggtcgc gccgaacccg cggcgcagcg gcagcaccac
     9781 cacgtagccg cgccgcgcaa acaccgcggc ggaatggatc agcaggtcgg cggattcgtt
     9841 ggcgcgcccg ctcgcggagc cgggcgtgcc gtgattgagg acgacggcgc cgaagggacc
     9901 gtcgccttgg ggcctgagga tggtcgctgc gatggtgtag cggccggcgg ggatgcggac
     9961 ttcttcctgc acgaggcgcg gaccgaagct gccttcatct gccagagctg cgcccgccat
    10021 ccagaggaat agccaggcga acactctcag aatctccccc ttgcgcactg tcgctccccc
    10081 cgcactggtg atttcgacta cgaactatag ccattaaata cgacgcggtg tagcggaaac
    10141 ttgtagtcgt tctgtagagt gaaaatccgc gtgaaaaatg tcgcccccgc gcgacaggga
    10201 aagctcgcag tgacagccga tttacgcagt ttgctccgac gtagtggcca aaccctggta
    10261 gcgccgggcg cgagtgacgc tctcagcgcg cgcctcatcg aagcgcacgg ctacgagtgc
    10321 gtctatatcg ggagctatgc gacggcggcg agccgcttcg ggctccccga taccgggcgc
    10381 ttgtcgttgg aacaactagc cgcgcaggcg aaaacgatcg tggacgcagt gcgcatcccg
    10441 gtaatcgccg acgccgaagg cttcgataac gccgtccccg ccttcgaagc ggccggcgtg
    10501 ggcgcgatcc atatcgagga ccacactggc gccgggaaac acaccagcgc tcctcagaaa
    10561 cttcatccaa tgaaggaagc tgcggagcgg attcgccaag ccgtcgcggc gcgcaagaac
    10621 ccggacttcc tgatcgtcgc gcgctcggat gcgttctggg tgcaccgcga cctcgaggac
    10681 tgcatcgcgc gcctcgagct ctacgcaagc gccggcgccg acatggtgtt cccaacgatg
    10741 gtgggaccgg gcgagctggc cgaggtccgt cgccgggtgg caagaccgct gatgatcgtc
    10801 gacaaccccg aggaacagca cgagggcgcc gcgatcgtcc tctactatgc cttttcgatc
    10861 acccggcaat tcgccgccct gcagcaggct ctggctacac gcaagctcgc gagcgacacc
    10921 gcgccgctcg aagactttct cggttataaa gacgccccag ggaggaaccg atgagatttt
    10981 ttctcgcagt tctgctcttg gcgtccggta gcgcgttcgc ccagggaagc tatccaaaca
    11041 agcccattcg cttgatcgtc ggcttcccgc cgggcggctc ggccgacccc accacgcgca
    11101 tcatcggcgc cgcgctgcag gagcagctcg gccagccggt ggtggtcgag aaccggcccg
    11161 gcgccgacag cgcgatcgcg gccgagcaga tgacgcgcat gccgcccgac ggctacaccc
    11221 tcatgttcgc ctcgaacagc gcgatgaccg cggcggtggc gctgcggaaa gcgccgacct
    11281 acgacccgtt gaaggacttc accccggtgt cgatggtcgg gcgcgcgacg gtgttcttct
    11341 acgtgcaccc ttcgatcccg gcgaagacgc tgaaggaatt catcgcgcac gccaaggcca
    11401 atcccgggaa ggtggtctac ggcaccggca acccgctctc gatcctctac ggtcagcagc
    11461 tcacgagcgc gaccggcatc gattggctgc acgtccccta caagggcgaa gggccgctca
    11521 accccgacat cctcgccggc cgcgtgcatt gctcgttcct cagcaccggc tcggcggtgt
    11581 cgaacgccaa ggacggccgc ctgcggccgc tcgcggtgct ggcggagaag cgcagcgcgc
    11641 tcctgcccga cgttcccacc atctccgaag ccggcgtgcc cgaggtgacc gtgcggcaat
    11701 gggccggcgt ctacggcccg ccgaagatgc cgcgcgagat cgtcgagcgc ctcaacaagg
    11761 aagtgaacgc ggcgctcaag cgcccagacg tgctcgaaaa gctgcagagc tacggctacg
    11821 ccgccgaggg ctcgaccccc gagcgcctgc tcgagatcaa ccgcgccgat ctcgagctgt
    11881 ggcgcaagct cgtgaaggag gcgggaatag cgctggagta agcgtgccgg gaaggtagag
    11941 cgggtgcgcg gggtggccgc tcccattcaa gcgcaggaag tgaagcggga tttttttctt
    12001 tctcaggatt tctcgcactt ccgcgccgcg cccgcgatgc tcgccatgct cgccccacgc
    12061 gcacaccacg agcttcgctt ccttcgccgc tcttagaatc gccgcgtcat ttcctcgtcc
    12121 gacgggattc ttcacaaccc tgagaactgc aggatcggtg gagcgccagg cgaagatgtt
    12181 cgtgatcacc accgcaccga atccccagcg ctcggcgtag ttgcgcgcgc gcgtgcacga
    12241 cgggtcgagc ttgaattcgt cggcggtgct cgggttgagc atcaggaagt tcgccgccgg
    12301 cttcgactcg tcccagcagc gccacagcag gtagcgccag cgccggcagg gcgagaacac
    12361 cgcgcccgct tcggtcattt cttcttcttc agcaggtccg ccaggccttc gaggggccgg
    12421 tgcttggcaa ccggggccgt ggctcggccc tggtttcccc acttcgcatc ttccacccgc
    12481 gcgtgctcgt tctcatggca gtagatgcac aagagctccc agttcgagcc gtcgggcgga
    12541 ttgttgtcgt ggttgccgtc cttgtggtgc accgtgagct cgcgcagccg cttgccttcg
    12601 aactcgcgcg cgcagcgcgt gcagatgtgc ggcaggagct tcaacgcttg agtgcggtag
    12661 tccacctcca tccggcgatt atctcctcgt gcgacctcgg ccttaagtta ccgcgctcgc
    12721 ttctcttacg cacctgactt ccgtcgccgc ggagcgcatt gtctgcatcg agatttcgaa
    12781 ccggtcggcg gcgcggtgca gacgaagcct ccggggaaag cgcgaggtgg cagtgccgtt
    12841 cacagggcat tagcgcgcgc gctgggcaac gccgccgtcg ccgccgaccg gttgcagtac
    12901 ggggtcagga cccttttttc tggacgcgcg gataaaaaag aacgtgaata agtcacaaat
    12961 cgcgtacgcc ttgctcacct gacttgtgcg actgcggcga gcactctcgg catcagaagg
    13021 gcggcaagcc agcaatacgg ggggaagcag cggccgcccg ccggcatccg gggggataca
    13081 agccggcgat ggagagacgt atagcgcgcg ctactttctg agcgcccgta gcagcccgcc
    13141 ccgcgacctc gggatcaggt cgctcggcgg gctgattttt ttcaggtcca cattccgcgc
    13201 agccgcgccg caacatccac cttgtagtcg gcagcagtcg tctcctcgga ctgggcgatt
    13261 tctccccacg ccagcggctc catgcacgcc agcaccgcct tcgtcatgaa gcggctcttc
    13321 gcgccgtaca catgccggtc gcccatgcgc gattggctcg ccacgtagta cttgagcggc
    13381 gcgaggacgt ggagctcgct cttcgcgcgc gtcatcgcca cgtagagcag gcgccgctcc
    13441 tcgtcgatca gctcgggccg gccggtggag aactcggagg ggaagttgcc gtcggccacg
    13501 ttgaggatgt gcaccgcctc ccactcctgc cctttcgccg agtgcaccgt cgagagcacg
    13561 aggtagtcct cgtcgaggta gggctcgccc gacaggtcgc tcgtcgcgct gggagggtcg
    13621 agggccattt cagtaaggaa taccgatctc tcgttaaact gagttgaaat cttctctagc
    13681 tggagcagat ccccggcccg cacctgcgcc gcatcgtaga tccgctcgag gtgcggcagg
    13741 taccactcgc gcacgcgctg cacctgcccc tcccaggggg cttctccaag gtagagcatc
    13801 aactcgtcga gcccggccga tccggtcttg agcggcttcc atgagaaccc
//
LOCUS       DATBSZ010000150        12269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2140128, whole genome shotgun sequence.
ACCESSION   DATBSZ010000150 DATBSZ010000000
VERSION     DATBSZ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12269
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140128"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1304)
                     /locus_tag="VIG70_09985"
     CDS             complement(102..1304)
                     /locus_tag="VIG70_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY8250925.1"
                     /translation="MENTHVDLADESKSRSPRGAAAKLANLSGDDIVRELIHLSPGFA
                     QDVLEALPTDARERATSAAPPDVAFQWQRNSLYDPGTIGRMMEPVVAAFPPDMKVGET
                     VEELRELVTRALITYVYVVDDKHRLLGIVTMRDLLFSGRERTLEEVMLRGAFALHAAM
                     PMKDAMRLVLDRHYPVYPVIDAERRLIGLVRGQTMFEAQAIEITLQAGSMVGLEKEER
                     LGTPWVHSLKLRHPWLQLNLLTAFLAAAVVGLFQDTIDRLVILALFLPVLAGQSGNTG
                     CQALAVTLRGMTLGELKPGTERPLVAKETWVGCLNGVGVGVAAAVGMYITATGQGQSQ
                     PVMLAFVVFLAMIASCMASGVSGAIIPLTLKRFGFDPATASSIFLTTATDVVSMGMLL
                     GLATILVR"
     gene            complement(1315..2700)
                     /locus_tag="VIG70_09990"
     CDS             complement(1315..2700)
                     /locus_tag="VIG70_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY8250926.1"
                     /translation="MKAQPKPNLVVILVDDLRFDETGASGHPYMKTPNIDRLAQEGAM
                     FANAFHTTPLCSPNRACIVTGQYASRHGIIDNVGREAMSHRLANYHLELQRLGYETAH
                     IGKWHMGNDAAPRPGYDTWISFRGQGTITDPVFWENGKETQQRGYVTDLLNDRAAQFV
                     ARSRKKPFALFLAHKAVHPDVQQKQDGTIDLASMRGYVLPERHEGLYRDGRYPPRPNV
                     LPVEKVLKEKPAWKEVFELRGRPESRPFLEGLHSGTQEEIRRRAAMMASVDEGVGLLL
                     DALERAGALENTLILFLGDNGFFFGEHGLGAERRFAYEEGIRTAFFVRYPKLVKPGTV
                     IEPMVIALDIAPTMIELARGRPGAQIQGRSFVPLLKGRGCVPWRKSFLIEYYNESAWP
                     WIVGMSYKAVRTERAKLIHWVHKDGVDELYDLKRDPYEIANVFKKPAYGKVRAQLYKE
                     LRMLVAEAAGL"
     gene            2742..3290
                     /locus_tag="VIG70_09995"
     CDS             2742..3290
                     /locus_tag="VIG70_09995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY8250927.1"
                     /translation="MTPRAITIRPAEQRDAPAIATMSRDFIESGLGWKYDAARVMRAM
                     RDRETLAVVACEGAKTSTAKGAVAGFAIMEFGDERAHLVLLAVRPSHRRLGIGQRLLD
                     WLLESARVAGMASIHLELRAGNDAARRFYRAMGFYETVLVPGYYRSGEGRKEGALRML
                     RVLRTPGPVPYTWRPPKTEDNT"
     gene            complement(3322..4398)
                     /locus_tag="VIG70_10000"
     CDS             complement(3322..4398)
                     /locus_tag="VIG70_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEY8250928.1"
                     /translation="MRAWVFLGMLLLPSAHAADWAIFSAEGQAEAGHRFEIAVLAPPG
                     EPHPDELAVRMKVDVAEIALTMQATGPAVDGRRVYAATMPVSASGPVTLRLADQPSNV
                     LVLVASRRDAVRSMTGSTIDEREPPLSENDPMYFIVGTRGGTTARFQLSFKYRLFDAS
                     TGFGQERPWLSGLYFGYTQNSLWDLSTESKAFRDTSYRPSLFWKWERAQERAFFDGAR
                     VGLEHESNGGQGDTSRSINIAFARPEWHWRVGQGSDLAFTPKVYAYLDKEENTDIDDY
                     RGYVDWRVRFESGNWIATSVLRYGTANKGSVLLDLSRRVRDMKFGPVSTYIHVQFFAG
                     YGESILDYNVKNKSQLRFGFAIVP"
     gene            complement(4395..5303)
                     /locus_tag="VIG70_10005"
     CDS             complement(4395..5303)
                     /locus_tag="VIG70_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8250929.1"
                     /translation="MDRLSAIQVFAQVVESGSFAKASARLGLSTSATSRHVAELEGHL
                     QTRLLNRTTRRVSLTESGRAFYERAVQLLADLEEAEQEAARAAVVPRGTIRLTTSVNF
                     GVRHLAPAIADFLAQHGEVRFDVSLSDRVVDLVEEGFDLGVRVGTAGSENLVARKLGE
                     TRLVPCAAPTYLAAHGAPEVPEDLERHNCFTYEYVTPRNLWRFRDSSGRERAVRVSGT
                     LHSNNGDLLAETAARGAGIVFEPAFIVGPDVRAGRLVPLLQDFTPPPVPIYAVYPSRK
                     HLSAKVRRFVEFLVTRFEHGQDWSAA"
     gene            5379..6239
                     /locus_tag="VIG70_10010"
     CDS             5379..6239
                     /locus_tag="VIG70_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY8250930.1"
                     /translation="MKNVLRIYRQPEGHWVGDGFPVHSVLDYQRHPELSPFLLLDHAG
                     PFDFPPAAKPRGVGWHPHRGFETVTVVLDGEVDHEDTAGNGGRIGRGDVQWMTAGSGL
                     LHKEMHSDAFTRRGGRFHALQLWVNLPAKSKMTPPRYQTLSREQIPSVALPEGNVRVI
                     AGEFRGVKGLAQTFTPVNLLEARVRAGQQLRLDLHEGFSAGLYIVEGKTTVNGEAANA
                     GELVVLEREGDGIVIDAATDAVVFVMNGAPIDEPVVGSGPFVMNSTREIQQAYADFHA
                     GQLGKIPALT"
     gene            6276..6893
                     /gene="gstA"
                     /locus_tag="VIG70_10015"
     CDS             6276..6893
                     /gene="gstA"
                     /locus_tag="VIG70_10015"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEY8250931.1"
                     /translation="MKLYYAPGACSLSPHIVSRELGIPVELKKVNTKDKTIEGGGDYW
                     QINGRGYVPALVLDDGQVLTEGPAIVQYLADQKPESGLAPKAGSLERYRLQEWLNFLT
                     SEVHKQFSPLFKPNTPEEYKKIVKDTLAARFDWLEKQLAGKDYLMGKQFTVADAYLFV
                     LLGWTRPTQIDLARWPNLAAFHKRVGARPKVQEALQAEGLLKKAA"
     gene            6896..7666
                     /locus_tag="VIG70_10020"
     CDS             6896..7666
                     /locus_tag="VIG70_10020"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY8250932.1"
                     /translation="MLPSVFISHGSPMHALEPGAAGEAWKALAQRLPRPRAILIASAH
                     WETNLPMLTGSDRPETVHDFYNFPEPLYRLRYPAPGAPEVAKRAQALLKAAGFTAGID
                     GCRGLDHGAWSPLLYMYPDADVPVVQISVQPALGPRHHVELGRSLRKLSEEDVLVIGS
                     GHMTHNLRDWARGQGQPAPYAREFQAWVFEKLKERDVDSLIDYRSRSPHGARAHPTDE
                     HFLPLFFALGAASEKAKPERVYDAIDSGVLAMDAYVFS"
     gene            complement(7675..8031)
                     /locus_tag="VIG70_10025"
     CDS             complement(7675..8031)
                     /locus_tag="VIG70_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8250933.1"
                     /translation="MADDLAELRALNGAYVRAVQHSDAAWFERHLAEDFVNTNPDCTL
                     SERPAFLERVAKPAGVSGLREEDVRIRILGDVALIHARTTYARTDGRPGAGRYTDIWQ
                     RRNGRWLCIAAHVSRG"
     gene            8086..8775
                     /locus_tag="VIG70_10030"
     CDS             8086..8775
                     /locus_tag="VIG70_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8250934.1"
                     /translation="MEDLPAITRIAALLADPARARMVWTLIDGTMRPAGELAYAASVS
                     AQSASMHLAKLVDGGLLAAQPQGRHRYFRLASPEVAGVVEDLASVGAAGRAREPRPAR
                     PAAAPAQFLIARTCYGHLAGETAVRILQAMLERRWLVPDGRDYVVTIAGERKLGALKI
                     DLATVRHSRRAFARPCVDLTQRRPHLGGALGDALLDIYVARGWVQRQRRSRAVNITPK
                     GSEAFRLAFGI"
     gene            8813..9466
                     /locus_tag="VIG70_10035"
     CDS             8813..9466
                     /locus_tag="VIG70_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HEY8250935.1"
                     /translation="MVGVSFLLPIWFDLAAVFLFALTGVWAATKRGYDVVGAFVLAFV
                     TGVGGGLLRDGIFLQEIPAVMKDARFLWVVIAALVIGGLAQRLADRFQMLIAYVDAIA
                     IGVYAVYGANKSLVAGVPPEAAVLVGLCNAVGGGLIRDVLVREEPLLLKPGQVYVLAT
                     LVGCAAFVVMSHRYGVDVEWAAYTAIAVTMLLRGLAIRFNWRTQAFALRFRRRDGSD"
     gene            complement(9463..10842)
                     /locus_tag="VIG70_10040"
     CDS             complement(9463..10842)
                     /locus_tag="VIG70_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY8250936.1"
                     /translation="MKRISFLVSVLLVGCSVQPKYEKPAAEVPDAWTETAPRFAEDGR
                     WWRIYQDPELDKTIDEALASNGDLLVAAARVDETRALLSEARSFFWPRIDAVGSVARQ
                     RNSERTAFSPPGIPNEFTTYRAALEVSYEADLFGRLRSNADAARAELEASEASREAVR
                     LALAAQVARSYFSLRSLDEQVALTRQTIVLREDALALQRKRFNGGVISEFELRQLEAE
                     AAVVRAQLPPLEALREREEVGLSVLLGRSPKQVFEGNVKVKVSMDEVPGPPAVPSGLP
                     SELLLRRPDLVEAERLLAASNARVGVARAEMFPSIVLTGALGSESSSLSDLFSGPSLI
                     WSLAAGIAQPIFQGGRLKARTEAAEARERAVLAQYQQAIRAAFGEVRAALIAQSRARE
                     TYDAESARATALAQTLRLARLRYQNGVSSQLDVIDAERGLLEAQRARIAALHAHRAAV
                     ADLFRALGG"
     gene            complement(10839..>12269)
                     /locus_tag="VIG70_10045"
     CDS             complement(10839..>12269)
                     /locus_tag="VIG70_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8250937.1"
                     /translation="EVSAAIRSNPFNEHVMAFTGFDFAGGFVFRENAATIFVTQTHWD
                     KRPMPVGALVGEFYGKTAHMKEGLAIAFPPPAIFGLGTAGGFEFYIQNRGEGGAKRLW
                     EVTQQFIGAASQSPKLGMVNTFWRANVPQLYVDVDREKAKSLGVPLDDIFSTLAATLG
                     SYYVNDFNKYGRTWQVLMSAESAQRGSPEDVGAVYVKSEKGEMIPLRSLARIEYSSGP
                     GTLTRFNNLPAVQVLGGGKPGVSSGDAIAEIERIAREVLPPEFSLDWSGASYQEKKSG
                     GTSVLVLALGALMAFLILAAQYEKWSLPLSVMLALPFGTFGALAAVWLRGLTNDVYFQ
                     IGLVTLLGLAAKNAILIVEFAVLKHEEGLSASAAAVEAARLRFRPILMTSLAFILGVL
                     PLALSSGAGAGARISAGTGVMGGMLAATFLAIFFVPTFFKLLTDRHLTEKRSSREIKD
                     EAAHARNVTHAPMQHPHHAPHGAGDE"
BASE COUNT         1989 a         4154 c         4143 g         1983 t
ORIGIN      
        1 ctcggtagac gcgcgcgccg ctctgcgccg cggctaaccg gggtgcgctc agcgcgagcg
       61 cgcccaccgc aagcaggaag gtcctgcggg aaatcgtgcc ttcagcgcac gaggatcgtt
      121 gcaagcccaa gaagcatacc catgctcacc acgtcggtcg cggtggtgag gaagatgctc
      181 gacgcggtcg cgggatcgaa gccgaagcgc ttcagcgtga ggggaatgat agcgcccgag
      241 acgccgctcg ccatgcacga ggcgatcatg gcgaggaata cgacgaaggc gagcatcacc
      301 ggctggctct ggccctggcc ggtggcggtg atgtacatcc ccacggcggc ggcgacgccg
      361 acgccgacgc cgttcaggca gccgacccag gtttccttgg caacgagcgg ccgctcggtg
      421 ccgggcttga gctcgccgag cgtcatgccg cgcagcgtca ccgcgagcgc ctggcagccc
      481 gtgttccccg actgaccggc gagcaccggc aggaaaagcg cgaggatcac gaggcgatcg
      541 atcgtgtcct ggaacaggcc cacgaccgcc gcggcgagga acgcggtcag caggttcaat
      601 tgcagccatg gatggcgcag cttgaggctg tgcacccacg gcgtgccgag gcgctcctcc
      661 ttctcgaggc caaccatgct gccggcctgc agcgtgatct cgatggcctg cgcttcgaac
      721 atggtctgtc cacgcaccag accgatcagg cgccgctcgg cgtcgatcac cggatacacc
      781 gggtaatgac gatcgagcac cagccgcatc gcgtccttca tcggcatggc ggcgtgaagc
      841 gcgaaggcgc cgcgcagcat cacctcctcg agcgtgcgct cgcggccgct gaacagaagg
      901 tcgcgcatgg tcacgatgcc gaggagacga tgcttgtcgt cgaccacgta cacataggtg
      961 atcagggcgc gcgtcaccag ctcgcgcagc tcctcgacgg tttcgccgac tttcatgtcc
     1021 ggcggaaatg ccgcgaccac cggctccatc atccggccga tggtgccggg gtcgtagagc
     1081 gagttgcgct gccactggaa ggcgacgtcg gggggcgcgg cggaggtggc gcgctcgcgc
     1141 gcgtcggtcg gcagcgcttc gagcacatcc tgcgcgaagc ccgggctgag gtggatcagc
     1201 tcccggacga tgtcgtcgcc ggagagattg gcgagcttgg ctgcggcgcc gcggggtgag
     1261 cgcgacttgc tctcgtccgc aagatcgaca tgggtatttt ccataatggc tcccttatag
     1321 ccccgcagcc tcggcgacga gcattcttag ttccttgtag agctgcgcgc gcactttacc
     1381 gtaggccggt ttcttgaaca cgttggcgat ctcgtacgga tcgcgcttga ggtcgtagag
     1441 ctcgtcgacg ccatccttgt gcacccagtg gatcagcttg gcgcgctcgg tgcgcaccgc
     1501 cttgtagctc atgccgacga tccagggcca ggccgactcg ttgtagtact cgatcaggaa
     1561 ggattttctc caggggacgc agccgcgccc cttgaggagc ggaacaaacg aacgcccctg
     1621 gatctgcgct cccggccggc cacgggcgag ctcgatcatg gttggcgcga tgtcgagcgc
     1681 gatcaccatc ggctcgatca ccgtgccggg cttgaccagc ttcgggtagc ggacgaagaa
     1741 agcggtgcgg atcccctctt cgtacgcgaa gcgccgctcc gcgccgagcc cgtgctcgcc
     1801 gaagaagaac ccgttgtcgc cgaggaacag gatcaaggtg ttttccaggg ctcctgcccg
     1861 ttcaagggcg tcaagaagca atccgacgcc ctcgtccacc gaggccatca tcgcggcgcg
     1921 gcggcggatc tcctcctgtg tgcccgagtg caggccttcg agaaacggtc ggctctcggg
     1981 ccgcccgcgc agctcgaaca cctccttcca ggccggcttc tctttcagga ctttttcgac
     2041 cggcagaacg ttcggccgcg gcggatagcg gccgtcgcgg tacaggccct cgtgacgctc
     2101 gggcaggaca tagccgcgca tgctggcgag gtcgatggtg ccgtcctgct tctgctgcac
     2161 gtcgggatgg acggccttgt gcgctaggaa cagcgcgaag ggcttctttc gggagcgagc
     2221 cacgaattgc gcggcgcgat cgttcaagag atcggtcacg tacccgcgct gctgcgtttc
     2281 ctttccgttt tcccagaaca ctgggtcggt gatcgttccc tggccgcgga agctgatcca
     2341 ggtgtcgtag cctggccgcg gtgcagcatc gttgcccatg tgccacttgc cgatgtgcgc
     2401 cgtctcgtag cccagccgct gcagctcgag gtgatagttg gcgagccggt ggctcatcgc
     2461 ctcgcggccg acgttgtcga tgatgccgtg gcgcgacgcg tactgcccgg tgacgatgca
     2521 ggcgcggttc ggcgagcaaa gcggcgtggt gtggaatgca ttcgcgaaca tcgccccctc
     2581 ctgcgccagg cggtcgatgt tcggcgtctt catgtaggga tgtcccgacg cgccggtctc
     2641 gtcgaagcgc aggtcatcca ccaaaatgac gacgagattg ggcttcggct gtgctttcat
     2701 aggcaggaca gcggccattt tctcatggca caatcccttc catgacgccc cgcgccatca
     2761 ccatccggcc ggccgagcag cgcgacgcac cagcgatcgc gacgatgtcg cgcgacttca
     2821 tcgagtccgg gctggggtgg aagtacgatg cggcgcgcgt catgcgcgcg atgcgcgacc
     2881 gcgagaccct cgccgtcgtg gcgtgcgaag gggcaaagac cagcaccgcc aagggcgcgg
     2941 tggcgggctt cgccatcatg gagttcggcg acgagcgtgc gcacctcgtg ctgctcgcgg
     3001 tgcgcccgtc gcaccggcgc ctcggcatcg gccagcgcct gctcgactgg ctgctcgagt
     3061 cggcgcgcgt cgccggcatg gcgagcatcc atctcgagct gcgcgccggc aacgacgccg
     3121 cgcggcggtt ctaccgcgcg atggggttct acgagaccgt gctggtgccg ggctactacc
     3181 gcagcggcga gggacgcaag gaaggcgcgc tgcgcatgct gcgagtcctg cgcacacccg
     3241 gcccggtgcc gtacacctgg cgcccgccga agaccgaaga caacacctag caattcgtta
     3301 gggctccgtc cccgaattgc cctacggcac gatcgcgaag ccgaagcgca gctgcgattt
     3361 gttcttcacg ttgtaatcca ggatcgactc gccgtacccc gcgaagaact gcacatgaat
     3421 gtaggtgctg accgggccga acttcatgtc gcggacgcgg cgtgacaagt cgagcagcac
     3481 gctgcccttg ttggccgtgc cgtagcgcag gacgctggtc gcgatccagt tgccggactc
     3541 gaagcgcaca cgccagtcga catagccgcg gtagtcgtcg atgtcggtgt tctcctcctt
     3601 gtcgaggtag gcgtagacct tcggcgtgaa cgcgaggtcg ctcccctggc caacccgcca
     3661 gtgccattcc ggccgcgcga aggcgatgtt gatcgagcgc gaggtgtcgc cctgcccgcc
     3721 gttcgactcg tgctcgaggc ccacgcgcgc gccgtcgaag aacgcgcgct cctgggcgcg
     3781 ctcccacttc cagaagagcg acggccggta gctggtgtcg cggaacgcct tcgattcggt
     3841 cgaaaggtcc cacagcgaat tctgcgtata gccgaagtag aggcccgaga gccaggggcg
     3901 ctcctggccg aagccggtgc tcgcgtcgaa caggcggtac ttgaacgaga gctggaagcg
     3961 cgctgtcgtt ccgccgcgcg tgccgacgat gaaatacatc gggtcgtttt ccgagagcgg
     4021 cggctcgcgc tcgtcgatcg tgctcccggt catgctgcgc actgcgtcgc gccggctcgc
     4081 gaccagcacc agcacgttcg agggctggtc ggcgaggcgc agcgtcaccg gcccgcttgc
     4141 cgagaccggc atggtcgcgg cgtagacgcg ccggccgtcg actgccgggc cggtggcttg
     4201 catggtgagc gcgatctcgg cgacgtccac cttcatgcgc acggcaagct cgtccggatg
     4261 cggctctccc ggcggcgcga gcaccgcgat ctcgaagcgg tgcccggcct cggcttgccc
     4321 ttcggcggag aaaatcgccc agtcggccgc gtgcgcgctc ggcaggagaa gcattccgag
     4381 gaacacccag gccctcacgc cgcgctccag tcctggccgt gctcgaagcg cgtgacgagg
     4441 aactcgacga agcgccgcac cttggcggag aggtgcttgc ggctcgggta gacggcgtag
     4501 atcggcaccg gcggcggcgt aaagtcctgt agcaggggaa ccaggcgccc tgcccgcaca
     4561 tcgggcccga cgatgaaagc cggctcgaaa acgatgccgg cgccgcgcgc agccgtctcg
     4621 gcgagcaggt cgccgttgtt cgaatgcagc gtgccggaaa ctcgcaccgc gcgctcgcgg
     4681 ccggaggaat cgcgaaagcg ccacagattg cgcggcgtta catattcgta cgtgaagcaa
     4741 ttgtgccgct cgagatcctc ggggacttcg ggcgcgccat gcgccgcaag gtaggtgggc
     4801 gcggcgcacg gcaccagccg ggtctcgccg agcttgcgcg cgaccaggtt ttccgagccg
     4861 gcggtgccga cgcgcacccc caggtcgaag ccttcctcca ccaggtcgac cacgcggtcg
     4921 gagagggaga catcgaagcg aacctcccca tgctgggcga ggaagtcggc gatcgccggc
     4981 gcgaggtggc gtaccccgaa gttcaccgag gtagtcagcc ttatcgtgcc gcgtgggact
     5041 accgcggccc gggccgcttc ctgctctgcc tcttccaggt ccgccaggag ctgtacggcg
     5101 cgctcgtaaa aggcacggcc gctttcggtg aggctgaccc tgcgggtcgt ccgattgagg
     5161 agccgggttt gcaggtgccc ctcgagctcc gccacgtgcc tcgaggtggc cgacgtggac
     5221 aggcccagcc gcgctgacgc cttggcaaag cttcccgatt caacgacttg ggcgaatacc
     5281 tggatggcgc tgagtcggtc cattgttgcg attaatggga ttatatcctc tatagataga
     5341 tctttatccc gaggagtctg gggctcatat tagtgcgcat gaaaaacgtc ctgcgcatct
     5401 accgccagcc cgaagggcac tgggtcggcg acggcttccc cgtgcattcc gtgctcgact
     5461 atcaacgcca tcccgagctt tcgccgttcc tgctgctcga ccacgcgggc ccgttcgact
     5521 ttccgccggc cgccaagccg cgcggcgtcg gctggcatcc gcaccgtggc ttcgagaccg
     5581 tcaccgtggt gctcgacggc gaagtcgatc atgaggacac cgccggaaac ggcggccgca
     5641 tcggcagggg cgacgtgcag tggatgaccg cgggatcggg cctgctgcac aaggaaatgc
     5701 acagcgacgc cttcacgcgc cgcggcggcc gcttccacgc gctgcagctc tgggtgaacc
     5761 tgccggcgaa gtcgaagatg acgccgccgc gttaccaaac cctttccagg gaacagattc
     5821 cttccgtggc gttacccgag gggaatgttc gggtcattgc aggcgagttc cgcggggtaa
     5881 aggggctggc gcaaacgttc acccccgtga acctgctcga ggcgcgggtg cgcgccgggc
     5941 agcagctccg cctggacctg cacgagggct tcagcgccgg gctttacatc gtcgagggaa
     6001 aaaccaccgt caacggcgag gccgcgaacg cgggcgagct ggttgtgctc gagcgcgagg
     6061 gcgacggcat cgtcatcgac gccgcgacgg atgcggtggt cttcgtgatg aacggcgcgc
     6121 cgatcgacga gccggtcgtc ggctccgggc cgttcgtcat gaacagcacg cgggagatcc
     6181 agcaggcgta cgccgacttc cacgccggcc agctgggaaa gatccctgca ctcacatgat
     6241 tacgaagtac tcacttacaa caggagactg actaaatgaa gctttactac gccccgggag
     6301 cctgctccct gtccccccac atcgtgtctc gcgagctcgg catccccgtc gagctcaaga
     6361 aagtgaacac gaaggacaag accatcgaag gcggcggcga ctattggcag atcaacgggc
     6421 gcggctacgt cccggcgctc gtgctcgacg acggccaggt gttgaccgaa ggcccggcga
     6481 tcgtgcagta cctcgccgac cagaagcccg agtccgggct cgcgcccaag gccggcagcc
     6541 tcgagcgcta ccggctgcag gagtggctca acttcctgac ctccgaagtc cacaagcagt
     6601 tctcgccgct tttcaagccg aacacgcccg aggagtacaa gaagatcgtc aaagacaccc
     6661 tcgccgcgcg cttcgactgg ctcgagaaac agctcgccgg caaagactat ctgatgggca
     6721 agcagttcac ggtggcggac gcgtatctct tcgtgctgct cgggtggacc cgacccacgc
     6781 agatcgacct cgcaagatgg ccgaacctcg ccgcgttcca caagcgcgtt ggcgcgcgtc
     6841 ccaaggttca ggaggcgctc caggccgaag gtcttctgaa gaaagcggcc tgagtttgct
     6901 tcctagcgtc ttcatctccc acggctcgcc gatgcacgcg ctggagcccg gcgcggccgg
     6961 cgaggcgtgg aaggcgctcg cgcagcgcct gccgcgcccg cgcgcgatcc tgatcgcctc
     7021 ggcgcactgg gagaccaacc tgccgatgct caccggctcg gataggcccg agacggtgca
     7081 cgacttctac aacttccccg agccgctcta ccgcctgcgc tatccggcgc ccggcgcgcc
     7141 ggaggtggcg aagcgcgccc aggccctgct caaggccgcc ggcttcaccg ccggcatcga
     7201 cggctgccgc gggctcgacc acggcgcctg gtcgccgctg ctctatatgt atccggacgc
     7261 ggacgtgccg gtcgtgcaga tctcggtgca gcccgcgctg gggccccggc accacgtcga
     7321 gcttggacgt tcactgagaa agttgagtga agaagacgtg ctcgtcatcg gctctggcca
     7381 tatgacgcat aaccttcgag actgggcacg tggccagggt caacccgccc cgtacgcgcg
     7441 cgagttccag gcctgggttt tcgagaaact gaaagaaaga gacgtcgaca gcctgatcga
     7501 ctatcgctcg cgctcgccgc acggcgcgcg ggcacatccg accgacgagc acttcctgcc
     7561 gctcttcttc gcgctcggcg ccgcttcgga aaaagcgaag cccgagcgcg tctacgacgc
     7621 catcgacagc ggcgtcctgg cgatggatgc ctacgtcttc agctagagga tcccctaacc
     7681 gcggctgacg tgcgccgcaa tgcagagcca gcggccgtta cgccgctgcc agatgtcggt
     7741 atagcggccg gccccgggcc ggccatcggt cctggcgtac gtggtgcggg cgtgaatcag
     7801 cgcgacatcg cccaggatcc ggatgcgcac gtcctcctcg cgcagcccgc tcacccccgc
     7861 gggtttcgcg acgcgctcga ggaaggccgg gcgctcggac agcgtgcagt cggggttggt
     7921 gttcacgaaa tcctccgcca ggtgccgttc gaaccaggcg gcatcggaat gctgcacggc
     7981 gcgcacgtag gcgccgttca acgcgcgcag ctctgcgaga tcgtcggcca tatggacagc
     8041 attctatgcc tgcgctaact tagacgtttc acggagcgct gaactatgga agacctgccc
     8101 gccatcaccc gcatcgccgc cctgctcgcc gacccggcgc gcgcgcgcat ggtctggacg
     8161 ctgatcgacg gcacgatgcg gcccgcggga gagctcgcgt acgccgcgag cgtctcggcc
     8221 cagtcggcga gcatgcacct tgcgaagctc gtcgacggcg gcctgctcgc cgcgcagccg
     8281 cagggccgcc accgctattt ccgcctcgcg agccccgaag tcgccggggt ggtggaagac
     8341 ctcgcgtccg tcggcgcagc cgggcgcgca cgcgagccga ggccggccag gccggcggct
     8401 gcgcctgcgc agtttctgat tgcccgaacc tgctacggcc atctcgcggg ggaaacggcc
     8461 gtccgcatcc tgcaagccat gctcgagcgt cgctggctcg tgccggacgg acgtgactac
     8521 gttgtgacca tagccggaga gcggaagctc ggcgcgctca aaatcgatct cgcgacggtc
     8581 cgacacagcc ggcgggcatt cgcgcgcccc tgcgtagatc tcacgcagcg ccgcccgcat
     8641 ctgggcgggg cgttaggcga cgcgctgctc gacatctatg tcgcccgcgg ctgggtccag
     8701 cggcagcgcc ggtcgcgggc cgtcaacatc acgccaaaag gaagcgaagc cttccgcctg
     8761 gccttcggca tctagcgctt cttcgttcgc aaggcacgcg tagtattgcg gcatggtagg
     8821 cgtctcattc ctgctgccga tctggttcga tctcgccgcc gtgttcctgt tcgcgctgac
     8881 cggagtctgg gcggcgacca agcgcggcta tgacgtggtc ggcgctttcg tgcttgcctt
     8941 cgtcaccggc gtcggtggcg gcctgctgcg cgacggcatc ttcctgcagg aaattccggc
     9001 ggtgatgaaa gatgcgcgct ttctctgggt ggtgatcgcc gcgctggtga tcggcgggct
     9061 ggcgcagcgc ctcgccgatc gcttccagat gctcatcgcc tacgtcgacg cgattgcgat
     9121 cggcgtctac gcggtctatg gcgccaacaa gtcgctggtc gccggcgtgc cgccggaagc
     9181 cgcggtgctg gtcggcctgt gcaacgcggt cggcggcggg ctgatccgcg acgtgctggt
     9241 gcgcgaagag ccgttgctgc tcaagcccgg gcaggtctac gtcctcgcca cgctcgtcgg
     9301 ctgcgctgcc ttcgtggtga tgagccaccg ctacggcgtc gacgtcgagt gggcggcgta
     9361 caccgcgatc gccgtcacga tgctgctgcg ggggctcgcc atccgcttca actggcggac
     9421 gcaggcgttc gccctgcgct ttcgccgccg cgacggctcc gactagccgc cgagcgccct
     9481 gaagaggtcc gccacggcgg cgcggtgagc gtgcagcgcc gcgatgcgcg cgcgctgcgc
     9541 ctcgagcagg ccgcgctcgg cgtcgatgac gtcgagctgg ctcgaaacgc cgttctggta
     9601 gcgcagccgc gcgaggcgca gggtctgggc gagcgccgtg gcgcgcgccg attcggcatc
     9661 gtaggtttcg cgcgcgcgcg actgcgcgat gagcgcggcg cgcacttcgc cgaacgcggc
     9721 gcggatcgcc tgctggtatt gcgcgagcac ggcgcgctcg cgcgcctcgg ccgcctccgt
     9781 gcgcgccttc aaccggccac cctggaagat cggctgggcg atgccggcgg cgagcgacca
     9841 gatgagcgac ggcccggaaa agaggtcgga gagcgacgag ctctcggagc cgagcgcgcc
     9901 ggtgagcacg atcgacggga acatctcggc gcgcgccacg ccgacacgcg cgttggacgc
     9961 cgccagcagc cgctcggcct cgaccaggtc ggggcgccgc agcagcagct ccgagggcag
    10021 gccggagggc actgcgggcg gtcccggcac ctcatccatt gaaactttga ctttgacgtt
    10081 tccctcgaaa acctgcttcg gtgagcggcc gagcagcacc gaaagcccca cctcctcgcg
    10141 ctcgcgcagg gcctcgagcg gcggaagctg cgcgcgcacc accgccgctt cggcttccag
    10201 ctggcgcagc tcgaactccg agatcacgcc gccattgaag cgcttgcgct gcaatgcgag
    10261 ggcatcttcg cgcaagacga tggtctgccg ggtcagggcc acctgctcgt cgagcgagcg
    10321 cagcgaaaaa tacgatcgcg cgacctgcgc ggccagggcg aggcgcaccg cctcgcgcga
    10381 ggcttcgctc gcctcgagct cggcgcgcgc cgcatcggcg ttggagcgca ggcgtccgaa
    10441 gaggtcggcc tcgtacgaca cctcgagcgc cgcgcggtag gtggtgaact cgttcgggat
    10501 cccgggcggc gagaacgcgg tgcgctcgga gttgcgctgg cgcgccaccg agcccacggc
    10561 gtcgatgcgc ggccagaaga acgagcgcgc ctcgctgagc agcgcacgcg tctcgtcgac
    10621 gcgcgccgcg gcgaccagca ggtcgccgtt gctggcgagc gcttcgtcga tcgtcttgtc
    10681 gagctccggg tcctggtaga tgcgccacca gcggccgtcc tcggcaaagc gcggcgcggt
    10741 ttccgtccag gcatcgggca cctccgccgc cggcttctcg tacttgggct gaaccgaaca
    10801 gcccaccagc agaaccgaaa ccaggaatga aatcctcttc attcgtcccc cgctccatgg
    10861 ggcgcgtgat ggggatgctg catgggcgcg tgggtgacat tgcgcgcatg cgcggcttcg
    10921 tccttgatct cccggctcga gcgcttctcg gtgaggtggc ggtcggtcaa cagcttgaag
    10981 aacgtcggca cgaagaagat cgcgaggaag gtcgccgcga gcatcccgcc catcacgccg
    11041 gtgccggcgg agatgcgcgc gccggcgccg gcgccgctgg agagcgcgag cggcagcacg
    11101 ccgaggatga acgcgagcga ggtcatcagg atcggccgga agcgcagccg cgccgcctcc
    11161 accgcggcgg ccgaggccga cagcccctct tcgtgcttga gcaccgcgaa ctcgacgatc
    11221 aggatcgcgt tcttcgccgc gaggccgagc agcgtcacca gcccgatctg aaagtagacg
    11281 tcattggtga ggccgcggag ccatacggcg gccaaagcac caaaagtccc gaacggcagc
    11341 gcgagcatca ccgagagcgg cagcgaccac ttctcgtact gcgcggcgag gatcaggaac
    11401 gccatgagcg cgccgagggc gagcaccagc accgacgtgc cgccggattt cttctcctgg
    11461 tacgacgcgc cgctccagtc gaggctgaac tcgggcggca gcacttcgcg cgcgatgcgc
    11521 tcgatctcgg cgatcgcgtc gcccgagctc acgccgggct tgccgccgcc caggacctgc
    11581 accgcaggca ggttgttgaa gcgcgtgagc gtcccggggc cgctcgagta ttcgatgcgc
    11641 gcgagcgagc gcagcggaat catctcgccc ttctcgcttt tcacatagac ggcgcccaca
    11701 tcctccgggc tgccgcgctg cgccgactcg gccgacatca gcacctgcca ggtgcggcca
    11761 tacttgttga agtcgttgac gtagtagctc cccagcgtcg cggcgagcgt gctgaagatg
    11821 tcgtcgagcg gcacgccgag cgacttcgct ttctcgcggt ccacgtccac gtagagttgc
    11881 ggcacgttgg cgcgccagaa ggtgttcacc atcccgagct tcgggctctg cgacgcggcg
    11941 ccgatgaact gctgcgtcac ctcccagagc cgcttcgcgc cgccttcgcc gcggttctgg
    12001 atgtagaact cgaagccgcc cgcggttccc aggccgaaga tcgccggcgg cgggaacgcg
    12061 atcgccaggc cttccttcat gtgcgcggtc ttgccgtaga actcgcccac cagcgcgccc
    12121 accggcatcg ggcgcttgtc ccagtgcgtc tgcgtgacga agatggtcgc ggcgttctcg
    12181 cggaacacga agccgccggc gaaatcgaag ccggtgaagg ccatcacgtg ctcgttgaac
    12241 gggttggagc ggatcgcggc gctcacctc
//
LOCUS       DATBSZ010000151         5098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1185229, whole genome shotgun sequence.
ACCESSION   DATBSZ010000151 DATBSZ010000000
VERSION     DATBSZ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185229"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VIG70_10050"
     CDS             <1..612
                     /locus_tag="VIG70_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEY8250938.1"
                     /translation="LVEKVEQVVRLIRSKGVGVYFVTQNPLDVPESVLGQLGNRVQHA
                     LRAFTPRDQKAVKTAADTLRPNPKLKAEQVITELGVGEALVSLLDEKGRPTIVERAFI
                     VPPASQVGPIGMEERAQMMRASSLLSKYQKTVERESAYEKLKGRAEEKPVVQEEQKKP
                     AGRQPQSVFDAATKSAARAIGSELGRQIIRGVLGSILGSGRRR"
     gene            609..1508
                     /locus_tag="VIG70_10055"
     CDS             609..1508
                     /locus_tag="VIG70_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8250939.1"
                     /translation="MNEGSYRAWGILFALIGVLAFSFRPILIKLSYAAHPVSPVTLLF
                     LRMTLSLPFFLAIAWWLRNTEPRLTRRDWAGIAGLGFLGYYGASFLDFIGLQWVGAGV
                     GRLILYLFPTLVVLLSFLFLRKTPGPRELVALVLSYAGIALVVSNQLAVTPEGKFFAF
                     GVLLIFASALCYAVYLVIGSQMVKRVGSMRFTAYSTVVATVPAVIQFLFMESMASLDL
                     PGAVWTYAIILATASTVLPLFLQAEALNRIGANHFALIGAVGPVSVAVTSALGLDEPF
                     TGVQALGGALVIFGVLLVSLKKG"
     gene            1511..2287
                     /locus_tag="VIG70_10060"
     CDS             1511..2287
                     /locus_tag="VIG70_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8250940.1"
                     /translation="MDLGIRGKTALVCAASKGLGKGCALALAREGVNLVITARGQEAL
                     EATATELKKFGVNVKTVAGDITTPLGREAALRAAPAPDILVNNAGGPPPGDFRGWDRD
                     AWVKALEANMITPILLIRDVVDGMVARKFGRIVNITSGAVKAPIAELGLSNGARTGLT
                     GFIAGLSRTTVRHNVTINNLLPGPFETDRLRSNLEFNAKKLGKTADELRKARTDANPA
                     GRFGTIEEFGDACAYLCSAQAGFITGQNLLMDGGGYPGTF"
     gene            2288..3088
                     /locus_tag="VIG70_10065"
     CDS             2288..3088
                     /locus_tag="VIG70_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY8250941.1"
                     /translation="MEVRRISGALGAELLGVDLSKDFPEKEIRKAFLEHQVVFFRDQE
                     LGPAQFMAFARRMGRPIEYPFVKGIEGFPEVIEVKKLEHERHNFGGIWHSDTAYLEEP
                     PMGSMLLAREVPAYGGDTEFASQYLAHEALSEGMKRVLEKLVAVNSSAKADVTRTRED
                     RVKHYSEHYEAEHPVVRTHPETGRKALYVNFGHTVRFRGMTEAESAPLLEFLFRHQVK
                     LEFTCRFHWEVGSLAFWDNRCTQHNPVNDYHGHRRVMHRITLAGDKPK"
     gene            complement(3161..>5098)
                     /locus_tag="VIG70_10070"
     CDS             complement(3161..>5098)
                     /locus_tag="VIG70_10070"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY8250942.1"
                     /translation="PAYHHPVFKAQYVVASISTLGYWTPMRMAGAQARRVLVDAAATK
                     WGVPAAECVTEPSTVVHQPSGRKMSYGEIASFATPPATPPAIDATKDLKKPGDYRLLG
                     KGVPRVDVPAKATGAAKYGIDARVPGMAYATFVRSPVRGSGPASSNAADVKKLPGIVD
                     VVTLDHGVAVVGETFAAVSKARKQLKVTWRSGQPGDAINTDKDLETYLGDGRDPKRVG
                     VEWKSKGDPAKAIGGAARTVSREYLSDHVYHAQMEPMNATADVRRDGIDIWAGTQAPT
                     RTTLDVAKALGTTPDMVRVHQQYLGGGFGRRATVEASVDAALISKAVNRPVKLILSRE
                     DDLQAGTFRPMTSQRLDVGVDAAGKIVAWQHRVVGEPVGDFVYHPGYNKAAKDRDTIF
                     MMGAELPYYNKIDHWRSEHLMEPERTRVAAWRGIGAGYTKFAIESMIDELAHAAKMDP
                     LAYRLAHTDDGRARRVLEKVAEMSGWGKKPAAGRARGIAFAEYGILAPKVGSLVAAVA
                     EISVNEKTGRIRVHNYWAAADAGLAINPPAFVGQMESAIVWGLSCALKERVTMVNGVV
                     QQSNFHQYELLRMSEVPEIKVEIITGAAPIPSMVGELGVPCTAPAVASAFFALTGRRL
                     YHMPFTPARVQAALKAKRIEA"
BASE COUNT          794 a         1725 c         1715 g          864 t
ORIGIN      
        1 ctggtcgaga aagtcgagca ggtggtgcgt ctgatccgct ccaagggcgt gggcgtgtac
       61 ttcgtgaccc agaacccgct cgacgtgccc gaaagcgtgc tcggccagct cggcaaccgc
      121 gtgcagcacg cgctgcgcgc cttcaccccg cgcgaccaga aagcggtaaa gaccgccgcc
      181 gataccctgc gccccaatcc caagctcaag gccgagcaag tgatcaccga gctcggcgtc
      241 ggcgaggcgc tggtcagcct gctcgacgag aagggccgcc cgacgatcgt cgagcgcgcc
      301 ttcatcgtgc cgccggcgag ccaggtcgga ccgatcggca tggaggagcg cgcgcagatg
      361 atgcgcgcct cctcgctgct ctcgaagtac cagaagaccg tcgagcgcga atcggcctat
      421 gaaaagctga agggccgcgc cgaggaaaaa ccggtggtcc aggaagagca gaagaagccc
      481 gccggccgtc aaccgcaaag cgtcttcgac gcggccacca agagcgcggc gcgcgccatc
      541 ggctcggagc tcggccggca gatcatccgc ggggtgctcg gctcgatcct cggcagcggc
      601 cggcgccgtt gaacgaaggt tcgtatcggg cgtgggggat cctgttcgcg ctcatcggag
      661 tgctggcgtt ctcgtttcgg ccgatcctga tcaagctctc gtacgccgcg cacccggtca
      721 gcccggtcac gctgcttttc ctgcgcatga cgctgtcgct gcccttcttc ctggcgatcg
      781 cctggtggct gcgcaatacc gagccgcgcc tcacgcggcg cgactgggcg gggatcgccg
      841 gcctcggctt cctcgggtac tacggggcga gcttcctcga cttcatcggc ctgcagtggg
      901 tcggggccgg cgtcggccgg ctgatcctgt acctctttcc cacgctcgtg gtcctgctct
      961 cgtttctctt cctgcgaaag acccccgggc cccgcgagct cgtggccctg gtgctgagct
     1021 atgccggaat cgcgctcgtg gtttcgaacc agctcgccgt cacgcccgag gggaaatttt
     1081 tcgccttcgg cgtgctgctc atcttcgcct cggcgctctg ctacgcggtg tatctcgtca
     1141 tcggcagcca gatggtgaag cgcgtcggat cgatgcgctt taccgcgtat tccaccgtgg
     1201 tcgccacggt gccggccgtg atccagtttc ttttcatgga atcgatggca tcgctggatt
     1261 tgcccggcgc ggtgtggacc tacgccatca tcctcgcgac cgcgagcacg gtgctgccgc
     1321 tcttcctgca ggccgaggcg ctgaaccgca tcggcgcaaa ccactttgcg ctcatcggcg
     1381 cggtcgggcc ggtgagcgtg gcggtcacca gcgcgctcgg gctggatgaa cccttcaccg
     1441 gggtgcaggc gctgggcggg gcgctggtga tcttcggtgt tctcctggtt tctctcaaga
     1501 aaggctgacc atggatctcg gcatacgcgg caagaccgcg ctggtgtgcg cggcgtcgaa
     1561 aggcctcggc aagggttgcg cgctggcgct cgcgcgcgag ggcgtgaacc tggtgatcac
     1621 ggcgcgcggg caggaagcgc tcgaagccac cgcgacggag ttgaagaaat tcggcgtgaa
     1681 cgtaaaaaca gtcgcgggcg acatcacgac gcctctgggc cgcgaggcgg cgctcagggc
     1741 tgcgcccgcg cccgacatcc tggtgaacaa cgcgggcggc ccgccgccgg gggacttccg
     1801 cggctgggac cgcgacgcgt gggtgaaggc actggaagcg aacatgatca cgccgatcct
     1861 cctgatccgc gacgtggtcg acggcatggt cgcgcggaaa ttcgggcgca tcgtcaacat
     1921 cacttcgggc gcggttaagg ccccgatcgc cgagctcggc ctttcgaacg gcgcgcgcac
     1981 cggactcacc gggttcattg ccgggctgtc gcgcaccacc gtgcggcaca acgtcaccat
     2041 caacaacctg ctgcccgggc cgttcgagac cgaccggctg cgctcgaacc tggagttcaa
     2101 cgcgaagaag ctcggcaaga cggccgacga gctgcgcaag gcgcgcaccg acgcgaaccc
     2161 cgcgggccgc ttcggcacca tcgaggagtt cggcgacgcc tgcgcctacc tgtgcagcgc
     2221 gcaggccggg ttcatcaccg ggcagaacct gctcatggac ggcggcggct accccgggac
     2281 gttctagatg gaagtgcggc gcatctccgg cgcgctgggg gccgagcttc tcggcgtcga
     2341 cctctcgaaa gatttccccg agaaggagat ccgaaaggcg ttcctcgagc accaggtcgt
     2401 cttcttccgc gaccaggagc tcggccccgc acagttcatg gcgttcgcgc gccgcatggg
     2461 caggccgatc gagtatccgt tcgtgaaggg catcgagggc tttcccgagg tgatcgaggt
     2521 gaagaagctc gagcacgagc ggcacaattt cggcggcatc tggcactccg acaccgcgta
     2581 cctcgaggag ccgccgatgg gctcgatgct cctcgcgcgc gaagtgccgg cctacggcgg
     2641 cgacaccgag ttcgccagcc agtacctcgc ccacgaggcg ctgtcggagg ggatgaaacg
     2701 agttcttgaa aagctggtcg cggtgaattc ctcggcaaag gcagatgtca cacgcacccg
     2761 cgaggacagg gtcaagcact attcagaaca ctacgaggcg gagcacccgg tggtgcgcac
     2821 gcatcccgag accggccgca aagcgctgta cgtcaacttc ggccataccg tgcgcttccg
     2881 cggcatgacc gaggcggaga gcgcgccgct gctcgagttc ctgttccggc accaggtgaa
     2941 gctcgagttc acctgccgct tccactggga agtcggctcg ctcgcgttct gggacaaccg
     3001 ctgcacccag cacaacccgg tcaacgacta ccatggccac cgccgcgtga tgcaccgcat
     3061 aacgttggcc ggcgacaagc ccaaatagaa tcagggacag accacgtttt cccggattcc
     3121 cgggaagagg aaaacgtggt ctgtccctga tttgctttct ttaggcttct attctcttgg
     3181 ccttgagcgc ggcctggacg cgggccgggg tgaacggcat gtggtagagc cgccggccgg
     3241 tgagcgcgaa gaacgcgctc gcgaccgccg gggcggtgca cggcacgccg agctcgccca
     3301 ccatgctcgg gatcggcgcg gccccggtga tgatctccac cttgatctcc ggcacctcgg
     3361 acatgcgcag cagctcgtac tggtggaagt tcgattgctg caccacgccg ttgaccatcg
     3421 tcacgcgctc cttcagcgcg cacgacaggc cccagacgat cgcgctttcc atctggccga
     3481 cgaacgcggg cggattgatg gcgagaccgg cgtcggctgc cgcccagtag ttgtgcaccc
     3541 ggatgcggcc ggtcttctcg ttgaccgaga tctcggcgac cgcagcgacc agcgagccga
     3601 ccttgggtgc gaggatgccg tattcggcga aagcgatgcc gcgcgcccgc ccggcagcgg
     3661 gcttcttgcc ccagcccgac atctcggcca ccttctccag cacgcgacgt gcgcggccgt
     3721 cgtcggtgtg cgccaggcga taggcgagcg gatccatctt cgccgcgtgc gccagctcgt
     3781 cgatcatcga ctcgatcgcg aacttggtgt agcccgcgcc gatgccgcgc cacgccgcca
     3841 ctcgggtgcg ctcgggctcc atcaggtgct cggagcgcca gtggtcgatc ttgttgtagt
     3901 agggaagctc ggcgcccatc atgaagatgg tgtcgcggtc cttcgccgcc ttgttgtagc
     3961 cggggtgata gacgaagtcg cccaccggct cgccgaccac gcggtgctgc caggcgacga
     4021 tcttgccggc cgcgtcgacg ccgacgtcga gccgctgcga ggtcatcggg cggaaagtgc
     4081 ccgcctggag atcgtcctcg cggctgagga tcagcttcac cggccggttc accgccttcg
     4141 agatgagcgc agcgtcgacc gaggcttcga cggtcgcgcg ccggccaaag ccgccgccga
     4201 ggtactgctg gtgcacgcgc accatgtccg gcgtggtgcc gagggccttc gccacgtcga
     4261 gcgtcgtgcg cgtgggtgcc tgcgtgccgg cccagatgtc gatgccgtcc cgccgtacgt
     4321 cggcggtggc gttcatcggc tccatctgcg cgtggtagac gtgatcgctg aggtactcgc
     4381 ggctcacggt gcgtgcggcg ccgccgatcg ccttcgctgg gtcgcccttc gacttccatt
     4441 ccacgccgac gcgcttggga tcacggccgt cgccgaggta ggtctcgaga tccttgtcgg
     4501 tgttgatcgc atcgcctggc tgaccgctgc gccaggtgac cttgagctgc ttgcgcgcct
     4561 tgctcaccgc cgcgaaggtc tcgccgacca ccgcgacgcc gtgatcgagc gtcaccacgt
     4621 cgacgatgcc gggaagcttc ttcacgtcgg cggcgttcga ggaagcgggg ccgctgcccc
     4681 tgacgggcga gcgcacgaaa gtggcgtacg ccatgccggg aacgcgagcg tcgatgccgt
     4741 atttcgccgc acccgtcgct ttcgccggca cgtcgacgcg cggcacgccc ttgccgagca
     4801 gccgatagtc gcccggcttt ttcaggtcct tggtggcgtc gatcgccggc ggcgtcgccg
     4861 gcggagtggc gaaggacgcg atctcgccgt agctcatctt ccggccgctg ggctggtgca
     4921 cgacggtgct cggctcggtg acgcactcgg ccgcgggaac gccccacttc gtcgcggcgg
     4981 cgtcgacaag cacgcgccgc gcctgcgcgc ccgccatgcg catcggcgtc cagtagccga
     5041 gcgtcgagat gctcgccacc acgtactgcg ccttgaacac cgggtggtgg taggcggg
//
LOCUS       DATBSZ010000152         4486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_501763, whole genome shotgun sequence.
ACCESSION   DATBSZ010000152 DATBSZ010000000
VERSION     DATBSZ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501763"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VIG70_10075"
     CDS             complement(<1..764)
                     /locus_tag="VIG70_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY8250943.1"
                     /translation="MKLLARALALCLVPLAAQAQNLSIATGGTGGVYYPLGGGMAAVL
                     SKYVQGMQATAEVTGGSVANLQLISTGKPYLAMTMVDAGLDALKGQDKFTGKPVPVRT
                     LMVMYPNRMHVVTIEGTGITKMADLKGKRVSTGSGGSATEVMAFRVIEAAGLDKDKDM
                     KRERLGVAESGNALKDRKIDAFFWVGGLPTAAVSDLAHSPGVKVKLIDHGDLAEKMNA
                     KYGKLYSPSKIKAGSYPGYDKDNSITEVWNLIVTGD"
     gene            complement(792..1400)
                     /locus_tag="VIG70_10080"
     CDS             complement(792..1400)
                     /locus_tag="VIG70_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8250944.1"
                     /translation="MAHHDFSTPIGEAQARALRVNDTVTLNGALFGIRDATQIHMFDR
                     GRKTRFDLRGHAVIHTAPNVRKVDKDPGNPAGYAPICVGTTTSDRMERFTRPLMREYG
                     VRLVIGKGGLRSDSGKAFQELGGAYLAIIGGTAALETTWIEAIEDVDLDDLNPESLWK
                     FRVNGFGPLLVAMDSHGESLYSRVKEASAARRVEALRKLGVR"
     gene            complement(1400..1903)
                     /locus_tag="VIG70_10085"
     CDS             complement(1400..1903)
                     /locus_tag="VIG70_10085"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEY8250945.1"
                     /translation="MAGVKIDDSTRVSNVELTLNGAGLRKRVFFQVYAIGLYLPQKSS
                     GAAEILQQPGPKRVAIHMLRDVGADTFTEALVDGIRANHSEAEAKALEPRIKELAAII
                     AELKEAKKGMAIALEWQPPTGTVLLVNGAARGKPIAGEDFYRALLRIWIGDKPVQDDL
                     KKGLLGG"
     gene            complement(1988..2863)
                     /locus_tag="VIG70_10090"
     CDS             complement(1988..2863)
                     /locus_tag="VIG70_10090"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEY8250946.1"
                     /translation="MKVDLAQVEEAAKELYIRALKALPPDIKRGLATLAASETDAGAK
                     RMLGTMIRNIAVAEDTDNLLCQDTGIPVYNVWIGRGVEVDGVGLKEAIRRGCARSTRE
                     YSFRSSIVHPTTRVNEQDSTGRGIPVINVDFVSEPDLLSIEMIPKGSGSENNSWLRMA
                     VPADGVDAIKTFVVDCVLEAGGKTCPPTIVGVGVGGTADLCVHLAKVAATRPLGTRCE
                     DPEGAKLEAALGEAVNLLGVGPQGLGGDSTAFAVHVETAATHITMNPVAVNMQCHSAR
                     RARATIRPAGVEYGF"
     gene            complement(2860..3816)
                     /locus_tag="VIG70_10095"
     CDS             complement(2860..3816)
                     /locus_tag="VIG70_10095"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY8250947.1"
                     /translation="MKKILVLVLGAFLVSTAGAQSWPQKPVRFIVPFPPGGATDISAR
                     LLGEKLSQIWGQTVVIENRGGAGGGLGAAEAARAAPDGYTLFFPSGSVVTANQHIYAK
                     MAYDPDKDFVPITKVVSGPQVLTVPASSRFKSVKELIDYARANPGKLTFGHAGIGSQT
                     HLAAENFVWQAKIEAVAVPYKGEGPGLAAAVAGETNFFLGNVAAALGHITSGRLRAIG
                     VTSRDEAAQLPNVPPIGKTIPGFENTGWFGLVAPTGTPRAILQKVYVDTKKALEASDL
                     RGRLYVQGLAPVGNTPDQMAREMKAESALWARVVKERKIEVR"
     gene            complement(3827..4141)
                     /gene="sdhC"
                     /locus_tag="VIG70_10100"
     CDS             complement(3827..4141)
                     /gene="sdhC"
                     /locus_tag="VIG70_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY8250948.1"
                     /translation="MRQHPSYWTFVVHRVSGIALAVFLPLHFWTLGRALALEEFIAWS
                     QQPLVKLGEWLIVVALAAHLGGGLRVLALEFLAWREWQKSLAAAAAALAIGVGLAFAL
                     AL"
     gene            complement(4138..4476)
                     /locus_tag="VIG70_10105"
     CDS             complement(4138..4476)
                     /locus_tag="VIG70_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEY8250949.1"
                     /translation="MSFKTQAALWIAQRASAAVLAVCVVVHLVTIVYAVQGGLTAAEI
                     LGRTRGNAGWFAFYSVFVLAVTVHVPIGLRSVCSEWLRWRGRSRDLALLLFAAFLAWA
                     GMRAVVGVFA"
BASE COUNT          695 a         1537 c         1497 g          757 t
ORIGIN      
        1 ttgtcgccgg tgacgatcag gttccagact tcggtgatgg agttgtcctt gtcgtagccg
       61 ggatacgagc ccgccttgat cttgctgggt gaatagagct tgccatattt ggcgttcatc
      121 ttctcggcca gatcgccgtg gtcgatcagc ttgaccttca cgccgggcga gtgggcgaga
      181 tcgctcacgg ccgccgtcgg caggccgccg acccagaaga acgcgtcgat cttgcggtcc
      241 ttgagcgcgt tgcccgactc ggccacgccg aggcgctcgc gcttcatgtc cttgtccttg
      301 tcgagccccg cggcctcgat gacgcggaac gccatcacct cggtggcgct gccgcccgaa
      361 cccgtggaca cgcgcttgcc cttcaggtcg gccatcttgg tgatgcccgt tccctcgatc
      421 gtcacgacgt gcatgcggtt cgggtacatc accatcagcg tgcgcaccgg gaccggcttg
      481 ccggtgaact tgtcctggcc tttgagcgca tcgaggccgg cgtcgaccat ggtcatggcg
      541 agatatggct tgccggtgct gatgagctgc aggttggcga cggagccgcc ggtcacttcg
      601 gcggtggcct gcatgccctg cacgtacttg gacagcacgg ccgccatgcc gccgccgagc
      661 gggtagtaga ccccgccggt gccgccagtg gcgattgaaa ggttctgcgc ctgcgccgca
      721 agcggtacca ggcagagcgc tagcgctctc gctaagagct tcattcgact cctcctatgg
      781 ttttggaaat tctaccgtac gcccagcttg cggagggctt cgacccgccg cgccgcggaa
      841 gcctccttca ctctcgaata gaggctctcg ccgtggctgt ccatagcaac gagcagcggg
      901 ccgaatccgt tgacgcggaa cttccacagc gactcggggt tgaggtcgtc gaggtcgacg
      961 tcctcgatcg cctcgatcca ggtggtctcg agcgccgcgg tgccgccgat gatggccagg
     1021 taggcgccgc cgagctcctg aaaggccttg cccgagtcgg agcgcagccc ccctttcccg
     1081 atcacgaggc gcaccccgta ttcgcgcatc aagggccggg tgaagcgctc catgcggtcg
     1141 gaagtcgtcg tgccgacgca gatcggcgca tagcccgcgg ggttgccggg gtccttgtcc
     1201 accttccgga cgttgggcgc ggtatggatc accgcgtggc cgcgcaggtc gaagcgcgtc
     1261 ttgcggccgc ggtcgaacat gtggatctgc gtcgcgtcgc ggatgccgaa gagcgcgccg
     1321 ttcaaggtca ccgtgtcgtt gacgcgaagc gccctcgcct gcgcttcgcc gatcggcgtg
     1381 gaaaaatcgt ggtgcgccat cagccgccca gcagcccctt cttgaggtcg tcctgcaccg
     1441 gcttgtcgcc gatccagatg cgcagcagcg cgcggtagaa gtcctctccc gcgatcggct
     1501 tgccgcgcgc cgcgccgttg acgagcagca ccgtgcccgt gggcggctgc cattcgagcg
     1561 cgatcgccat tcccttcttc gcctctttca gctccgcgat gatcgcggcg agctccttga
     1621 tgcgcggctc gagcgccttc gcctcggcct cggagtggtt ggcgcggatg ccgtcgacga
     1681 gcgcttcggt gaaggtatcg gcgccgacgt cgcgcagcat gtggatcgcg acgcgcttcg
     1741 gccccggctg ctggaggatc tcggcggcgc ccgagctctt ctgcggcagg tagaggccga
     1801 tggcgtagac ctggaagaag acgcgcttgc gcaggccggc gccgttcagc gtcagctcga
     1861 cgttggagac gcgggtgctg tcgtcgatct tgacgccggc cacttccgcc gccgatgccg
     1921 ccaaggcagg gaggaagaaa aacggcaaaa ggatagccgc tgtgagtctc gtaatcatgg
     1981 caacctccta gaagccgtac tccacccccg ccggacgaat ggtggcgcgc gcgcggcgcg
     2041 cggagtggca ctgcatgttg actgcgaccg ggttcatggt gatgtgcgtg gccgcggtct
     2101 cgacgtgcac cgcgaacgcg gtcgagtcgc cgcccaggcc ctggggcccg acgccgagca
     2161 ggttgaccgc ctcgccgaga gccgcctcga gcttcgcgcc ctcggggtcc tcgcagcgcg
     2221 tgccgagcgg ccgggtcgcc gcgaccttgg cgaggtgcac gcacagatca gcggtgccgc
     2281 cgacgcctac gccgacgatc gtcggcgggc aggtcttgcc gccggcttcg agcacgcagt
     2341 cgacgacgaa ggtcttgatc gcgtccacgc cgtcagccgg caccgccatg cgcagccagg
     2401 agttgttctc ggatcccgag cccttcggga tcatctctat ggacagcaaa tcgggttcag
     2461 aaacgaagtc cacgttgatc accgggattc cccgcccggt cgagtcttgc tcgttgacgc
     2521 gcgtggtggg atggacgatc gacgagcgga acgagtattc gcgcgtcgag cgcgcgcagc
     2581 cgcggcggat cgcttctttc aggccgacgc cgtcgacttc cacaccgcgc ccgatccaca
     2641 cgttgtagac cgggatgccg gtgtcctggc acagcaggtt gtcggtgtcc tcggcgaccg
     2701 cgatgttgcg gatcatggtg ccgagcatgc gctttgcgcc cgcgtcggtc tccgaggccg
     2761 cgagcgtcgc gaggccgcgc ttgatgtcgg gcggcagcgc cttcagggcg cggatgtaaa
     2821 gctccttcgc cgcctcctcg acctgggcaa gatcgacttt cacctcacct cgatcttgcg
     2881 ctccttcacc acacgagccc agagcgcgct ctccgccttc atctccctgg ccatctggtc
     2941 cggcgtgttg ccgaccggag cgagcccctg cacgtagagc cgcccgcgca ggtcgctcgc
     3001 ctcgagcgcc ttcttggtgt cgacatagac cttctgcagg atggcccgcg gcgtcccggt
     3061 cggcgcgacc aggccgaacc agccggtgtt ctcgaatccg gggatggtct tgccgatcgg
     3121 cggcacgttg ggaagctgcg cggcctcgtc cctgctcgtc acgccgatcg cgcgcaggcg
     3181 cccgctagtg atgtgtccga gggcggcagc gacgttgccg aggaagaagt tcgtctcgcc
     3241 ggccaccgcc gcggcgaggc cgggcccttc gcctttgtac ggcaccgcca ccgcttcgat
     3301 cttcgcctgc cagacgaagt tctcggcggc taggtgggtc tgcgagccga tgccagcgtg
     3361 gccgaacgtg agcttgccgg gattcgctcg cgcgtagtcg atgagctcct tcacgctctt
     3421 gaaacgcgag ctcgccggga cggtaagcac ctgcggcccc gacacgacct tggtgatcgg
     3481 cacgaagtcc ttgtcggggt cgtacgccat cttggcgtag atgtgctgat tcgcggtgac
     3541 caccgagccc gagggaaaga agagcgtgta gccgtcgggc gcagcgcgcg ccgcctccgc
     3601 tgcgccgagg ccgccgccgg cgccgccgcg gttctcgatc accacggttt gcccccaaat
     3661 ctgcgagagc ttctcgccga gcagccgcgc cgagatgtcg gtggcgccgc cgggcgggaa
     3721 cggcacgatg aagcgcaccg gtttctgcgg ccaggattgc gcgcccgcgg tggagacgag
     3781 gaacgcgccc agcaccaaca caagaatttt cttcatggcg aagattctac agcgccaggg
     3841 cgaacgccaa ccctacgccg atcgcgaggg ccgccgccgc cgcggccagg ctcttctgcc
     3901 attcgcgcca ggcgaggaac tcgagcgcga gcacgcgcag cccgccgccc aggtgcgcgg
     3961 cgagcgcgac cacgatcagc cactcgccga gcttcaccag cggctgctgg ctccatgcga
     4021 tgaactcttc caatgcgagg gcgcggccga gagtccagaa atgcagcggc aggaacacgg
     4081 ccagggcgat gccggatacg cggtgcacca cgaacgtcca gtacgaggga tgctgtctca
     4141 agcgaaaacc ccgaccaccg cgcgcattcc cgcccacgcg aggaaggcgg cgaacagcag
     4201 cagcgcgagg tcgcgcgagc gcccgcgcca gcgcagccat tcgctgcaca cgctgcgcag
     4261 gccgatcggc acgtggaccg tcaccgccag cacgaacacg gaatagaagg cgaaccagcc
     4321 ggcgttgccg cgcgtgcggc ccaaaatctc ggccgccgtc agcccgccct ggaccgcgta
     4381 gacgatggtg acgagatgca cgacgacgca cactgccagc acggccgcgc tcgcgcgctg
     4441 cgcgatccac agggccgcct gcgtcttgaa gctcaaagct cgccca
//
LOCUS       DATBSZ010000153         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1185901, whole genome shotgun sequence.
ACCESSION   DATBSZ010000153 DATBSZ010000000
VERSION     DATBSZ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185901"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VIG70_10110"
     CDS             <1..921
                     /locus_tag="VIG70_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEY8250950.1"
                     /translation="PSMPVWMVRNPTHGNRTFSNLNEGLGKVLRFGANGPDVIARLKW
                     MEKTLAPTLQAGLEVLKDLEIKPLMAQALHMGDEVHNRNGAASALLIKRLVPALLKSS
                     AAPADAAAVIEFIAGNDHFFLNISMAACKAMLDAAHGIAGSSMVTAMARNGVEFGIRV
                     SGTGERWFTAPAPLVNGLYFPNQSIKDAAPDLGDSAITETAGVGGFAMAAAPAIVKFV
                     GGSAQDALANTLAMTHITLRRNPAFTLPALDFAGTPACIDARRVVDTGILPVINTGIA
                     HKEPGVGQIGAGVTRAPLACFTQAILALAS"
     gene            918..>1552
                     /locus_tag="VIG70_10115"
     CDS             918..>1552
                     /locus_tag="VIG70_10115"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEY8250951.1"
                     /translation="MKDQGLAVLAIGGNALIRDRKHESIPNQYDMVCALAEDLAGMLE
                     SGWNIVVTHGNGPQVGFILRRSELSIAEVPPVPMDYAGADIQGAVGYMFVKALRNAFR
                     RRGLKRDAIALVTQTIVDRDDPAFANPLKPIGSHMEEARAKQLAAKYRWTVREDAGRG
                     WRRVVPSPQPKAIVETGVIRALASQGYVVVACGGGGIPVIEDQNGDLQGVEA"
BASE COUNT          249 a          552 c          537 g          214 t
ORIGIN      
        1 ccgtcgatgc cggtgtggat ggtgcgcaac ccgacgcacg gcaaccgcac cttcagcaat
       61 ctcaacgagg gcctgggcaa ggtgctgcgc ttcggcgcca acgggccgga cgtcatcgcg
      121 cgcctgaagt ggatggagaa aacgctcgcg ccgacgctcc aggccgggct cgaggtgctg
      181 aaggatctgg agatcaagcc gctgatggcg caggcgctgc acatgggcga cgaggtgcac
      241 aaccgcaacg gcgccgcgtc cgcgctcctc atcaagcggc tggtcccggc gctgctcaag
      301 tcgagcgccg cgccggcgga tgccgcggcg gtgatcgagt tcatcgccgg caacgaccac
      361 tttttcctga acatttcgat ggccgcctgc aaggcgatgc tcgacgccgc gcacggcatc
      421 gccggcagca gcatggtgac cgccatggcg cgcaacggcg tcgaattcgg catccgggtc
      481 tcgggcacgg gcgagcgctg gttcaccgca cccgcgccgc tggtgaacgg gctctacttc
      541 cccaaccagt cgatcaagga cgccgcgccc gacctgggcg acagcgcgat caccgagacc
      601 gcgggcgtcg gcggcttcgc catggcggcg gcgccggcga tcgtgaagtt cgtcggcggc
      661 tcggcgcagg acgcgctcgc caacacgctc gcgatgacgc acatcaccct gcggcgcaac
      721 cccgccttca ccctgcccgc actcgacttc gccggcacgc cggcgtgcat cgacgcgcgc
      781 cgcgtggtcg acaccgggat cctcccggtg atcaacaccg gcatcgcgca caaggagccc
      841 ggcgtcggcc agatcggcgc gggcgtcacg cgcgccccgc tcgcgtgctt tacgcaggcg
      901 atcctcgcct tggcctcgtg aaggatcagg gtctcgcagt gctggcgatc ggcggcaacg
      961 cgctgatccg cgaccgcaag cacgagtcga tcccgaacca gtacgacatg gtgtgcgcgc
     1021 tggccgagga cctcgccggc atgctggaga gcggctggaa catcgtcgtc actcacggca
     1081 acgggccgca ggtcggcttc atcctgcggc gctcggagct ctcgatcgcc gaggtgccgc
     1141 cggtgccgat ggactacgcc ggcgccgaca tccagggcgc ggtgggctac atgttcgtga
     1201 aagcgctgcg caacgcgttc cggcgccgcg gcctgaagcg cgacgcgatc gcgctggtca
     1261 cccagaccat cgtcgatcgc gacgatccgg cgttcgccaa tccgctcaag ccgatcggct
     1321 cgcacatgga agaagcgcgc gcgaagcagc tcgccgcgaa ataccgctgg accgtgcgcg
     1381 aggacgcagg ccgcggctgg cggcgcgtcg ttccctcccc gcagccgaag gccatcgtcg
     1441 agaccggcgt gatccgcgcg ctcgcgagcc agggctacgt ggtcgtcgcg tgcggcggcg
     1501 gcggcatccc ggtgatcgag gaccagaacg gcgatctcca gggcgtcgag gc
//
LOCUS       DATBSZ010000154         3106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_981084, whole genome shotgun sequence.
ACCESSION   DATBSZ010000154 DATBSZ010000000
VERSION     DATBSZ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981084"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VIG70_10120"
     CDS             <1..141
                     /locus_tag="VIG70_10120"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY8250952.1"
                     /translation="FVKDRPGHDRRYAIDSSKIARELGWRPRESFESGLATTIRWYIQ
                     NR"
     gene            138..1016
                     /gene="rfbA"
                     /locus_tag="VIG70_10125"
     CDS             138..1016
                     /gene="rfbA"
                     /locus_tag="VIG70_10125"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEY8250953.1"
                     /translation="MKGIVLAGGSGTRLYPATQVVSKQLLPVYDKPMVYYPLSTLMLA
                     GIRDILLISTPQDTPRFEALLGDGKKWGLRLAYAVQPRPEGIAQAFLIGREFIAGARV
                     ALVLGDNIFHGHDFSELLKRAAARERGATVFAYAVNDPARYGVVEFDASGKAVSIEEK
                     PRQPKSRYAVTGLYFYDTRVGEIAARLKASARGELEITDLNRAYLERGELEVIPMGRG
                     MAWLDTGTHEALLEAAHFIETIERRQGLKIACPEEIAYRMGFIDAAQLEALAKPLEKS
                     GYGEYLRRVLTDRLLP"
     gene            1013..1558
                     /gene="rfbC"
                     /locus_tag="VIG70_10130"
     CDS             1013..1558
                     /gene="rfbC"
                     /locus_tag="VIG70_10130"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEY8250954.1"
                     /translation="MKVTHTDIADVLVFEPRVHQDARGFFLESYNRRAFKEATGLDIK
                     FVQDNTSFSVRNVLRGLHYQIRQPQGKLIQVMAGEIFDVAVDLRRGSPTFRKWVGVTI
                     TGGGHRMVWIPEGFAHGYLVLSEHAIVLYKTTDYYAPEHERSILWNDAELGIRWPLEA
                     DPIVSDKDQRASPLRSADLFD"
     gene            1555..2391
                     /gene="rfbD"
                     /locus_tag="VIG70_10135"
     CDS             1555..2391
                     /gene="rfbD"
                     /locus_tag="VIG70_10135"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEY8250955.1"
                     /translation="MRILLTGRNGQVGSELERALPALGEVIASDRGTLDLADPGAIRR
                     VVREVKPGLIVNAAAYNAVDKAESETALAMQVNAEAPGMLAEEARRLGALMVHYSTDY
                     VFDGEKRMPYAEEDAPNPISYYGRTKLEGERTVAASGCRHLILRTSWVFGPRAANFYQ
                     IIQRKARAGESMRMVDDQTSVPTPSAFLAEHTVALLRADASGLLHLVPSGKATRYEFA
                     RAVVGAVGSTSHVDAARTTEFPTAARRPTYSVLDNRKLAALLARALPDWRELLPSLVE
                     RL"
     gene            2438..>3106
                     /locus_tag="VIG70_10140"
     CDS             2438..>3106
                     /locus_tag="VIG70_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8250956.1"
                     /translation="MQSYKPITGRRIRFGLVGCGRIWKNHVEALKKHSKDAEIVGVCD
                     VNEQALEAGEAATGTQGYLQYEEFLRKSEADVVVLATPSGLHTEQTIRAAQAGKHVIT
                     EKPMATRWQDGKRMVAACDQAAVRLFVVKQNRRNATLQLLKKAIEADRFGRIFMVAVN
                     VFWSRPQSYYDSAKWRGTWEFDGGAFMNQASHYVDLLDWLIGPVESVFAYTATLARTI
                     EVEDT"
BASE COUNT          547 a         1030 c         1048 g          481 t
ORIGIN      
        1 ttcgtgaagg accgtcccgg ccacgaccgg cgctatgcga tcgacagctc gaagatcgcg
       61 cgcgagctcg gctggcggcc gcgcgagagc ttcgagtccg gtctcgccac gacgattcgc
      121 tggtatatac agaaccgatg aaaggcatcg tcctcgccgg cggctcgggc acgcgccttt
      181 accccgcgac ccaggtcgtg tcgaagcagc tccttccggt gtacgacaag ccgatggtgt
      241 actacccgct gtcgactctc atgctcgccg gcatccgcga catcctgctc atctccacgc
      301 cgcaggacac gccgcgcttc gaggcgctgc tcggcgacgg gaagaagtgg ggcctgcgcc
      361 tcgcctatgc ggtgcagcct aggcccgagg gcatcgcgca ggcgtttctc atcggccgcg
      421 aattcatcgc cggcgcgcgt gtggcgctgg tgctgggcga caacatcttc cacggccacg
      481 acttcagcga gctcctcaag cgcgccgccg cgcgcgagcg cggcgccacc gtgttcgcct
      541 acgcggtcaa cgatccggcg cgctacggcg tggtcgagtt cgacgccagc ggcaaggcgg
      601 tcagcatcga ggagaagccg cgccagccca agtcgcgcta cgcggtcacc ggcctgtatt
      661 tctacgacac gcgggtcggg gagatcgcag cgcgcctcaa ggcctcggca cgcggcgagc
      721 tcgagatcac cgacctcaac cgcgcctacc tcgagcgcgg cgagctcgag gtgatcccga
      781 tgggccgcgg catggcgtgg ctggacacgg gaacccacga ggcgctgctc gaggcggcgc
      841 acttcatcga gacgatcgag cgccggcagg ggctgaagat cgcctgcccc gaagagatcg
      901 cctaccgcat gggcttcatc gatgcggcgc agctcgaggc gctcgccaag ccgctggaga
      961 agagcggcta cggcgagtac ctgcgccgcg tgctgaccga tcgtctcctg ccatgaaagt
     1021 cacgcacacc gacattgcgg acgtgctggt gttcgagccg cgcgtgcacc aggacgcgcg
     1081 cggctttttc ctcgagagct acaaccggcg cgccttcaag gaggccaccg gcctggatat
     1141 caagttcgtg caggacaaca cctccttctc ggtgcgcaac gtattgcgcg gcctgcacta
     1201 ccagatccgc cagccgcagg gaaagctgat ccaggtgatg gcgggcgaga tcttcgacgt
     1261 cgcggtggat ctgaggcgcg gctcgcccac gttccgaaag tgggtcggcg tcaccatcac
     1321 cggcggtggc caccgcatgg tgtggatccc ggaaggattc gcgcacggct acctcgtgct
     1381 atcagagcac gcgatcgtgc tctacaagac caccgactat tacgccccgg agcatgagcg
     1441 ctcgatcctc tggaacgatg ccgagctcgg catccgctgg ccgctcgagg ccgatccgat
     1501 cgtgagcgac aaagaccagc gcgcgagccc gttgcgctcg gccgacctgt tcgattgagg
     1561 attctgctca ccggccggaa cggccaggtc gggtcggagc tcgagcgcgc gctgcctgcg
     1621 ctcggcgagg tgatcgccag cgaccgaggc acactcgact tggcggatcc cggcgcgatt
     1681 cgccgagtcg tgcgcgaagt gaagccgggc ctgatcgtga acgctgccgc gtataacgcg
     1741 gtcgacaagg cggagtccga gaccgcgctt gcgatgcaag tcaatgccga ggcgcccggc
     1801 atgctggcgg aggaagcccg ccggctgggc gcgctgatgg ttcactattc caccgactac
     1861 gttttcgacg gcgagaagcg catgccgtac gcggaggagg acgcgcccaa tccgatcagc
     1921 tactacgggc gcacgaagct cgaaggcgag cgcaccgtcg cggcgagcgg atgccggcac
     1981 ctcatcctgc gcacgagctg ggtcttcggg ccgcgcgccg cgaacttcta tcagatcatc
     2041 cagcgcaagg cacgcgcggg cgaatcgatg cgcatggtcg atgaccagac tagcgtgccg
     2101 acgcccagcg ctttccttgc ggagcacacg gttgcgctgc tgcgcgccga cgcgagcggc
     2161 ttgttgcacc tcgtgccttc tggaaaggca acgcggtatg agtttgcgcg cgcagtcgtc
     2221 ggcgccgtcg gctcgacgtc gcacgtcgac gcggcgcgga ccaccgagtt tccgaccgcc
     2281 gcacgacgcc ccacgtactc cgtgctcgac aaccgcaagc tcgccgccct gctcgcgcgt
     2341 gcgctgccgg attggcgcga gttgctgccg tctctcgtcg agcggctata atccgtcccc
     2401 attcaagagg ttagaagccc atgagcgaga cctaagcgtg cagagctaca agccgatcac
     2461 cgggcgccgc atccgcttcg gcctggtggg atgcgggcgc atctggaaga accacgtcga
     2521 ggcgctgaag aagcattcga aggacgccga gatcgtcggc gtgtgcgatg tgaacgagca
     2581 ggcgctcgag gcgggcgaag cggcgacggg gacgcagggc tacctgcagt acgaggagtt
     2641 cctgcgcaag agcgaagccg atgtcgtcgt gctggctacg ccgagcggct tgcacacgga
     2701 acagaccatc cgtgcggcgc aggccggcaa gcacgtgatc acagagaagc cgatggcgac
     2761 gcgctggcag gacggcaagc gcatggtcgc agcctgcgac caggccgcgg tgcggctctt
     2821 cgtcgtcaag cagaaccggc gcaacgccac gctgcagctc ctgaagaagg cgatcgaggc
     2881 ggaccgtttc ggccggatct tcatggtcgc ggtgaatgtg ttctggagcc ggccgcagtc
     2941 ctactacgac agcgcgaaat ggcgcggcac ctgggagttc gacgggggcg ccttcatgaa
     3001 ccaggcgagc cactatgtcg acctcctcga ctggctgatc gggccggtgg agagcgtctt
     3061 cgcctacacc gcgacgcttg cgcgcacgat cgaggtcgag gatacc
//
LOCUS       DATBSZ010000155         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_707948, whole genome shotgun sequence.
ACCESSION   DATBSZ010000155 DATBSZ010000000
VERSION     DATBSZ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707948"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VIG70_10145"
     CDS             complement(<1..1341)
                     /locus_tag="VIG70_10145"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEY8250957.1"
                     /translation="MSTTKLGLAAACALAFPMLAQAAPGAAECTALAGLQDFPVQPTS
                     ITLTQFSEATATLPAHCRVQGTMEARTGVRGIPYGTRFEVRLPAEWNGRFMFQGGGGT
                     QGNLPAATGSAGTLSPTLAKGFAVATENGGHQNGDLTPDPNGMVITSAMFFDDPQAVR
                     DWSYRSVEITTRTAKHLIASFYGTPARHSYFVGCSTSGRQGIAMAQDFARHYDGIVAG
                     DPFFVPPHISLSETWTLQQVKSVSPVGADGKPIWHQSYSDTDRALFTRAILEACDGLD
                     GLEDGVIDNHAACRFDPAKFRFAATGEPLQCKGAKTDACLSPAQVKAIKKMEDGPRTS
                     WGAPIVAPNGWPVTGYPYDGGWMEPSGIPARNIGTPTSQPGNLTAGTAQLPLFWFTRP
                     DPTFDPVDFDWDRDMRLVVKRAAGVRDDTDLSDFRRRGGKIIFYHGLSDSGPPWT"
     gene            1460..>1623
                     /locus_tag="VIG70_10150"
     CDS             1460..>1623
                     /locus_tag="VIG70_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8250958.1"
                     /translation="MNLRQLDYFVHVADLGSFSRAASVLSVAQSALSHRVRQLEVELK
                     QPLLFRNGRGV"
BASE COUNT          241 a          529 c          580 g          273 t
ORIGIN      
        1 ggtccacggc gggccggagt cgctcaggcc gtgatagaag atgatcttgc cgccgcgccg
       61 gcggaagtcc gagaggtcgg tgtcgtcgcg cacgccggcg gcgcgcttga ccaccaggcg
      121 catgtcgcgg tcccagtcga agtccaccgg gtcgaacgtc ggatccggac gcgtgaacca
      181 gaagagcggg agctgcgccg tgccggccgt caggttgccc ggctgcgagg tcggcgtgcc
      241 gatgttgcgc gcgggaatgc ccgaaggctc catccagccg ccgtcgtacg gatagccggt
      301 gaccggccag ccattgggcg cgacgatcgg cgcgccccac gaggtgcgcg gcccgtcttc
      361 catcttcttg atcgccttca cctgggccgg tgacaggcag gcgtcggttt tcgcgccctt
      421 gcactgcaac ggttcgccgg tcgcagcgaa gcggaatttc gcgggatcga agcggcaggc
      481 ggcgtggttg tcgatcaccc cgtcctcgag cccgtcgagg ccgtcgcagg cctcgagaat
      541 cgcgcgggtg aagagcgcgc ggtctgtgtc cgagtagctc tggtgccaga tcggcttgcc
      601 gtcggcgccc accggcgaga ccgacttcac ctgctggagc gtccaggtct cgctgagcga
      661 gatgtgcggc ggaacgaaga acggatcgcc cgccacgatg ccgtcgtagt gccgggcgaa
      721 atcctgcgcc atggcgatgc cctggcggcc gctggtcgag cagccgacga agtacgaatg
      781 gcgcgccggc gtgccgtaaa agctcgcgat caggtgcttc gcggtgcgcg tagtgatctc
      841 gaccgagcgg tacgaccagt cgcgcaccgc ctgcgggtcg tcgaagaaca tggcgctggt
      901 gatgaccatg ccgttgggat cgggcgtcag gtcgccgttc tggtggccgc cgttctcggt
      961 cgccacggcg aagcccttgg cgagcgtggg cgagagcgtg ccggcgctgc cggtggccgc
     1021 gggcaggttg ccctgcgtgc cgccgccgcc ctggaacatg aagcggccgt tccactcggc
     1081 cggcaggcgc acctcgaagc gcgtgccgta cgggatgccg cgcaccccgg tgcgcgcctc
     1141 catggttccc tgtacccggc aatgcgccgg gagggtcgcg gtcgcctcgc tgaactgggt
     1201 cagggtgatg ctggtaggtt gaaccgggaa atcctgcagt ccggcgagcg ccgtgcattc
     1261 cgccgctccc ggggcagcct gcgcgagcat cggaaacgcc agcgcgcaag cggccgcaag
     1321 gccaagcttg gtggtagaca tcaacttctc cccctcggaa gtattacggt cccattcttc
     1381 gccatttgcc ggattatgga aatggcgata tcgcactagc tgctagcgcg gttcgagata
     1441 tcatgggtcg gctacgtcca tgaacctgcg ccagctcgat tacttcgtgc acgtcgcgga
     1501 cctcggcagc ttcagccggg cggcctcggt gctgtcggtc gcccagtcgg cgctcagcca
     1561 ccgggtgcgc cagctcgagg tcgagctgaa gcagccgctc ttgttccgta acggacgcgg
     1621 ggt
//
LOCUS       DATBSZ010000156        37726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1049807, whole genome shotgun sequence.
ACCESSION   DATBSZ010000156 DATBSZ010000000
VERSION     DATBSZ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 37726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37726
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049807"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VIG70_10155"
     CDS             <1..390
                     /locus_tag="VIG70_10155"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004552088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEY8250959.1"
                     /translation="EAGGISIGLNIRLPREQVANRYITPGLCFSFRYFALRKLHFLLR
                     ARAMVAFPGGFGTFDELFETLTLVQCRKIAPMPIVLVGKAYWRRAVDMEYLAAKGMIA
                     QRDRRLFRYAETAGEIWKAVAPNLATT"
     gene            complement(387..578)
                     /locus_tag="VIG70_10160"
     CDS             complement(387..578)
                     /locus_tag="VIG70_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250960.1"
                     /translation="MFSLFRSPGGEHYVDRGSVSCPRRGRDVEVDLCAGCEWMTRIDL
                     QAKPPFVRGRPAPVLRLRA"
     gene            complement(597..1049)
                     /locus_tag="VIG70_10165"
     CDS             complement(597..1049)
                     /locus_tag="VIG70_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8250961.1"
                     /translation="MGAYRKILVAVDGSPASSKGLREAIRLARAERAKLLILHVVDEF
                     QAFAAMDGLVGAPGADLLSALREGGKRILAKAVGIALKQKIKPKGILREMLSGPAAYP
                     IVREARKLRADLIVLGTHGRRGVRRLVLGSDAEQVVRSASVPVLLVRT"
     gene            complement(1049..1471)
                     /locus_tag="VIG70_10170"
     CDS             complement(1049..1471)
                     /locus_tag="VIG70_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY8250962.1"
                     /translation="MANITRYNPFEDFFQDFGKGFWLKPLPFPAETELKMKIDVKEDD
                     KSFTVKADIPGVKKEDIQIDIEDDHISLRAEVKQEKEEKKDEKVVYSERAYGMVSRSF
                     TLPVDVDAKAAKAEYRDGVLNLTLPKKGNGSAKRVAVS"
     gene            complement(1499..3112)
                     /gene="groL"
                     /locus_tag="VIG70_10175"
     CDS             complement(1499..3112)
                     /gene="groL"
                     /locus_tag="VIG70_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561123.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY8250963.1"
                     /translation="MTAKQILFGESAHARLIKGMNTLTDAVRVTLGPKARTVVLEKSW
                     GAPIVINSGVVVAKEIELEDRFENMGAQMVREVAAKTSEVAGDSTTTATLLAAAIVNE
                     GMKYVAAGMNPMDLKRGIDSAVEALIAELARMAKPCSTRNEIAQVGAISANNDASIGA
                     MIAEAMEKVGREGVITVEDGKGLANELEVVEGTQFDRGFLSPYFITNAEKQSVVLEDA
                     YILLHDKKIATIRELLPVLEHVAKTGKPLLVIAEEVEGEALATLVVNKLRGVLVSCAV
                     KAPGFGDRRKSMLEDLAVLTGGTVVAEEAGITLEKVDPAVLGRARRVEIDKDDTTIIG
                     GAGERKNIDARVAQIKAEIEEATSDYDKEKLQERAAKLAGGVALIRVGAATETEMKER
                     KSRTEDALHATRAAVEEGILPGGGVALLRARKRLASLQGENPDQDAGIRIVERAVEEP
                     LRQIVSNAGAEPAVVVQRVLDGEGNFGYNAATGVYGDMVAMGVLDPRKVTRTALQNAA
                     SIASLILTTDCMIAQIPDKPARAREEEMA"
     gene            complement(3109..3495)
                     /locus_tag="VIG70_10180"
     CDS             complement(3109..3495)
                     /locus_tag="VIG70_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY8250964.1"
                     /translation="MNAKPEEMFMRAWKAQLDAGLRAIETITESAIRMHEVQLSAATQ
                     AHADAEATRAVIAAATDPAQLTKLCAEWARSNAGKSFAYWRSLLQAGTPQAASGMLKQ
                     EMIDGAYQQWLDTVQRFYHPFEKSGS"
     gene            complement(3506..3829)
                     /locus_tag="VIG70_10185"
     CDS             complement(3506..3829)
                     /locus_tag="VIG70_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250965.1"
                     /translation="MLSLEDCLALCDLSEEEVLAIAHHERIPEIAAVELGNYLVHSPG
                     GEMRIKTIIRDDIDEARAQGDRLQELALKLVLRNFVLQHPRCEERHRRQLGAVERRDE
                     CAGPG"
     gene            complement(3822..4076)
                     /locus_tag="VIG70_10190"
     CDS             complement(3822..4076)
                     /locus_tag="VIG70_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250966.1"
                     /translation="MKNDSLQAGIEHRIADLRAAYPRISACSAKLEDWREAAEPRWSL
                     RIDVRWPQHQCLISGPARPSAEQAVQAAFDAAVRSLTSHA"
     gene            complement(4073..4612)
                     /locus_tag="VIG70_10195"
     CDS             complement(4073..4612)
                     /locus_tag="VIG70_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEY8250967.1"
                     /translation="MRKMLMIGAALALASSLAAAQGYGPGYGYGQGRGMMGGGYGAGM
                     MGGPGMGGYGPGMMGGPGMMGGPGMGGYGAGGMFAALNLSDEQREKISAIQEAQRQKN
                     WATMGAVRSEQFKLRGMYGTEKLDADKLVAQQKKVDELRQQMLKSRVDAHNQIAAVLT
                     PEQRKQLRQFAPWWLTEEE"
     gene            4730..5047
                     /locus_tag="VIG70_10200"
     CDS             4730..5047
                     /locus_tag="VIG70_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250968.1"
                     /translation="MPAAKRNIGDEMRGITRKALERGTLDRAAVRKVTGEVRKRLLQI
                     ERVFAQDLRVAARAGKDAAAQSMSATLVSGAIAARIASGMLAGIADSIARGTARRTPR
                     RRR"
     gene            5047..6696
                     /locus_tag="VIG70_10205"
     CDS             5047..6696
                     /locus_tag="VIG70_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEY8250969.1"
                     /translation="MLRALAGTARDLGRLQEIGAVLLRYGFGDLVGRLGIILHWKDSD
                     QPAQLGAPQRARLALQELGPAFVKLGQILATRVDLFPPEWIAQFEKLQDQAPPAAAHE
                     VRAQLVEDLGTAPEEAFAAFDPEPVAAGSIAQVHRARLADGTAVAVKVRRPGIVPVVE
                     ADLRLLARFAEILEARFPDVARFRPREVVRQFTRTLRTELDLAAECRNAERVAQNFRA
                     HPEIVVPAVYWAWTGERVNVQAFVDGTPSRDPEALEAAGLDRHALAHRGAQAMLKMVL
                     EDGFFHADPHPGNVFYLPGDRVAFIDFGMAGHLSERRRDELVQLLNGLVGRDAEAVVE
                     ILLDWAGAARVDAEKVSLDVDAFIDRYHGLPLHELRMGAMLADMTALLREHQLALPPD
                     LALVLKACVTLETYGRELDPQFDMVGEAAPFLQAATLERFGPARLAKRGWRAGRMLLE
                     VLSDLPRDLRELLRSAKTGKLQVHVDLTSLHRFGVQLDGAANRLAIGIVTAAFIVGSS
                     IVMTVEGGPTFFGLPLLGALGFSGALVGGAWLFVSILRSGR"
     gene            complement(6693..7352)
                     /locus_tag="VIG70_10210"
     CDS             complement(6693..7352)
                     /locus_tag="VIG70_10210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8250970.1"
                     /translation="MRTSFTVSRCVVFAAALAAASAAAQAPHTHRHGFGDAEKWAHVF
                     DDPERDAWQKPHQVIRALALEPDAIVADIGAGTGYFAARLANMLPKGTVYAVDIEPAM
                     VKYLGERAQREGLGNLKPVAGLPGDPRLPAKADLILLVDVYHHIEDRGRYFRSLAASL
                     KPGGRLAVIDFRLDSPNGPPKEARIAPERVKAEMKSAGYALAGEHGFLPNQYFLVFRP
                     G"
     gene            7458..7871
                     /locus_tag="VIG70_10215"
     CDS             7458..7871
                     /locus_tag="VIG70_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250971.1"
                     /translation="MTRYLPVLALAVFSAGAAAQPFPMNEHGDVRWVCAGIGEGEREA
                     LAKMEAGTSLKLVFTSGKRGEYLTDVDVVLSDREGKRPALKFIAEGPICLIQAPAGRY
                     QVEAAFGDTKRSVATTVTKGVKQPGMVVFRFPGTQ"
     gene            complement(7825..8484)
                     /locus_tag="VIG70_10220"
     CDS             complement(7825..8484)
                     /locus_tag="VIG70_10220"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8250972.1"
                     /translation="MGELERWNTRFGLPDYIFGTAPNEFLASKAALLVPGSRALCVAD
                     GEGRNSVWLAERGLEVVAFDLSPVGVEKARRLAEKRGVKVAFEVADVYGWRWPHAGFD
                     LVAAIFVQFADPAMRGFLFERIVRALKPGGLLLLEGYAPKQLEYRTGGPPHLENLYTP
                     QMLRQAFAALEILELKAYEAVLAEGTQHSGRSALVDLVARKPAGPIAFRGSETPPSRA
                     A"
     gene            complement(8472..8900)
                     /gene="trxC"
                     /locus_tag="VIG70_10225"
     CDS             complement(8472..8900)
                     /gene="trxC"
                     /locus_tag="VIG70_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEY8250973.1"
                     /translation="MPSDPIQVACPACLTANRVPAARLGEEPKCGKCGAALLPEAPLE
                     LAEAQFDDFVRRTELPVIADFWAPWCGPCRAMAPHFERAARELKGRARLVKVNTEEAP
                     GLAARMGIRAIPTIALFRNGVEVKRVSGALDAAALVRWAS"
     gene            8972..9283
                     /locus_tag="VIG70_10230"
     CDS             8972..9283
                     /locus_tag="VIG70_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8250974.1"
                     /translation="MIELDSMHEAAAEACRLMKALANPDRLMLLCQLSQGECSVGELE
                     AALGILQPTLSQQLGVLREEEIVQTRREGKHIYYRVHSPQALAVLQVLYDQFCRKGRK
                     R"
     gene            9280..9702
                     /locus_tag="VIG70_10235"
     CDS             9280..9702
                     /locus_tag="VIG70_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEY8250975.1"
                     /translation="MTLDWTHFTPWASAAGGALIGLAAAMLVLLNGRIAGISGIVGGL
                     LVPRGGDIGWRLAFVGGLLVAPVAMSFYEVNPRIDAGFGTLALAGVLVGIGTRYGSGC
                     TSGHGVCGLSRLSPRSLVATVAFMAAGVATVFIVRHLV"
     gene            9699..10136
                     /locus_tag="VIG70_10240"
     CDS             9699..10136
                     /locus_tag="VIG70_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEY8250976.1"
                     /translation="MNLLAAFGSGLVFGIGLILSGMTDPGKVIGFLDLAGNWDPSLAF
                     VMGGAILVGFFAFALAGRRSKTFLGAALHLPQRRDIDNRLVGGSLLFGIGWGLAGFCP
                     GPALVSFASGVDQAAVFVAAMLGGMLVYHAAERLIHEPRRRAT"
     gene            10153..10299
                     /locus_tag="VIG70_10245"
                     /pseudo
     CDS             10153..10299
                     /locus_tag="VIG70_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
     gene            complement(10263..10556)
                     /locus_tag="VIG70_10250"
     CDS             complement(10263..10556)
                     /locus_tag="VIG70_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250977.1"
                     /translation="MRAVSLLILLLPLCAFAQDAERGRVLYQTYCGGCHYERIHIERL
                     RPAVRDLADLRDMVAQWAPHTKQRVYSLEELEDMVQYLNESYYRIGLPPKKKQ"
     gene            complement(10690..11658)
                     /locus_tag="VIG70_10255"
     CDS             complement(10690..11658)
                     /locus_tag="VIG70_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8250978.1"
                     /translation="MKARLIAFSITTALLAGAWLTYQRDLAREVERVSTGSHIADTRC
                     GPIEYASLGSGPAVLLVHGAGGGFDQGLGIAQELAAHGLQVIAMSRFGYLRTPLPADA
                     SPAAQADAHACLLDALGVERAAIVGISAGAPSTMQFALRYPGRTDAMVLLVPLAYAPR
                     AAPAVPSRAARFMIEQATKSDLLYWAALKGAPSLIVKTVLATPPEVLATASADEQARV
                     RKLMLQILPLSARQPGLTNEARIAASLPRYDLERISTPTLIVSLVDDSYGTFESASYT
                     AAHIPGARFIGYGRGGHLWVGHHGEVMAKLIAFLGKAAMPLAALKR"
     gene            complement(11655..11984)
                     /locus_tag="VIG70_10260"
     CDS             complement(11655..11984)
                     /locus_tag="VIG70_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250979.1"
                     /translation="MSAKRPLTLAVMLLASCAWTPSSNWYLMDAGYSIDAVNSAEFVM
                     EVHVNQLRQLGGEIRSPEFRHFVAERVKWHGMCPSGWAFLPCIEDGSCVQRTNRSVTV
                     AGRCATS"
     gene            12327..14030
                     /locus_tag="VIG70_10265"
     CDS             12327..14030
                     /locus_tag="VIG70_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8250980.1"
                     /translation="MGVTLYVSAATGWRSTQALIGQQTEALLDALEQRLAARLRPVHE
                     QADWIAEAVNNGRIDLARPGELDSFMFGALGVTPQVSDIGIVDAAGRAQRWRRGELRA
                     LRDDWSSRPEVREWLAAGQVEPGRAWRPLLWKPAERIPSLLHQAPLHRAGQFVGMLGQ
                     VVPIAKVSEDLAVFRAEYEVTPFMLDSEDRVLGHPALAQRRADAPGAFPTLAELGDPV
                     LARLRSPDGAARLLSGLKRAQATRARVDGRIFVYVYREMAPHGGQPWTLGLYLDPVEG
                     GQRDEMLRVVWSIAAGLAVLVIAVTLAAVGGRRLGRPIESLARAAHLVRKGRLGEVQP
                     LPRSRIHEIDEANRSFGEMVEGLRERNLIRDTLGQFVPEQVAHELLAGGGRLEPVEAK
                     ATVLVCDIEGFAALTDELGARRTIEFLNAYFEVLVAIVERYQGVVTQFQGDAILAVFN
                     VPIPAADHGANALRAALEIVRAADSQAFAGVRARNRVGLSTGRVVAGAVGSAGRLSYT
                     VHGNAVNLAARLEQMNKEYGTRILLSDKTAERCPGFALRKVADAEIRGYGEPVPLYTP
                     G"
     gene            complement(14027..14422)
                     /locus_tag="VIG70_10270"
     CDS             complement(14027..14422)
                     /locus_tag="VIG70_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250981.1"
                     /translation="MTRSLVLALAFLAAEAAACGYCVEDKIASTYDHAVVTRALAQGH
                     HVVFFHIDGPPATQRELENAAASVPGVERGSVRLSLPTLTLSVAFDPRRVSLVELNAR
                     LDRRLAARKLSFMPMRIMEEPADLKTVKR"
     gene            complement(14428..15849)
                     /locus_tag="VIG70_10275"
     CDS             complement(14428..15849)
                     /locus_tag="VIG70_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY8250982.1"
                     /translation="MLSTVALVTLAACNPAAPPDRYDVSKMDLKPAPAKVTPERHKGA
                     FAEGAALSMGAELKPLDAAPVKTVRLDTTHKIIEVAPGVKFAAWTFGDQVPGPTVRAR
                     VGDRIRFVMTNRSDEAVAPVQVMAAPMMHSMDFHAAMVSPQDKYRSIAPGQTIEFEFT
                     ANYPGIFMYHCGTPMILEHIASGMYGAVIVDPREGYPTKAHREYVVVQSEFYLKPDPQ
                     KRKIDGAPLYVLDSERLRAAQPTHTVFNGSHNGMVRTPLAAKPGERVRMFVLNVGPSR
                     TSSFHVVGTIFDRVWMEGNPDNQLRGMQTVLLGSSNSATVEYLIPEEGSYIMVDHHFA
                     NASQGAIGLVSTTAKPPAKDLEHHNLQASATPTDPDAVAGKLAFESKCLACHTVGEGR
                     KLGPDVAGVTKRREQAWLARWLKEPEKMLQTDATAKAMLKEYNNIPMPNQSLTDAEVR
                     QYLKYFQWIDSQPAGAAKAQGGH"
     gene            complement(15842..16936)
                     /locus_tag="VIG70_10280"
     CDS             complement(15842..16936)
                     /locus_tag="VIG70_10280"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEY8250983.1"
                     /translation="MFAAGFRPFFLLASVWAAIAVPVWLAAYAHGYELRGGVSPMAWH
                     AHEMVYGFGLASVAGFLLTAIPNWTGRLPVRGAPLALLALLWLAGRVAMLVPEAAFID
                     LAFTAALIAVVARELVAGRNWRNLPMLVALLLLFAGNVLFHLQIYLGLRLGLATLLML
                     IALVGGRIVPSFTRNWLAKLGRGARLPAPEDRFDHLALLVTLAALGAWVAAGITWPLV
                     AAGVGLAARLSRWCGLSTVREPLLFILHLGYAWLALGLILLGLDVPGGAALHALTVGA
                     VGTMTLAVMTRASLGHSGRALVADGATVAIYALVTLAALLRVLSPLSGGQALVVTSLA
                     GLAWSAAFGLFALHYGRLLIQGSSYREKTA"
     gene            17020..17514
                     /locus_tag="VIG70_10285"
     CDS             17020..17514
                     /locus_tag="VIG70_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY8250984.1"
                     /translation="MRLTTFSDYSLRVLMFVAAAPEGRATISQVASAFNISEHHLVKV
                     VHFLGRHGILLNTRGRRGGLQLARPAHAINVGELVRLTEGGDMPAECFDKRTNTCALA
                     GGCRLQGMLKRAVASFYEALAPYSVADLQAPRRLARILPFHSASQCTASAQSISHRVI
                     NGVK"
     gene            17550..19718
                     /gene="katG"
                     /locus_tag="VIG70_10290"
     CDS             17550..19718
                     /gene="katG"
                     /locus_tag="VIG70_10290"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEY8250985.1"
                     /translation="MKVKDKSPGQCPFTHVAGVGRTNRDWWPKQLRLELLHQHSSKSD
                     PMGGGFDYAREFKSLDYKALKKDLVALMTDSQDWWPADFGHYGPFFIRMSWHAAGTYR
                     VTDGRGGAGRGQQRFAPLDSWPDNVNLDKARRLLWPVKQKYGKKISWADLLILAGNVA
                     LESMGFRTFGFGAGRADVWEPDEDVNWGEEIAWLGVDKRFSGDRDLGPFGATHMGLIY
                     VNPEGPNASGDYMAAAKDIRATFSRMAMNDEETVALIAGGHSFGKCHGAAPESHKGPD
                     PEGAPLEAQGLGWTSNYGSGHGADTVSSGLEVTWTKTPALWSNSFLELLYKYEWELTK
                     SPAGAKQWVAKNAPEIIPDAHIPGKFHKPTMLTTDCTLRFDPAFGKISRRFLDDPQAF
                     ADAFARAWFKLTHRDMGPIARYLGPEVPSEELIWQDPIPKVDHKLIGEKEIAELKKKI
                     LASKLSVAQLVSTAWASASTFRGSDKRGGANGARIRLAPQKDWAVNQPLAKVLKTLEG
                     IQKGFTKKVSLADLIVLGGCAAIEQAAAKAGVAVKVPFKPGRMDASQAQTDVESFAVF
                     EPIADGFRNYLKGQYPVSAEQLLLDKAQLLTLTAPEMTVLVGGLRMLGTNAGGVKHGV
                     FTSKPETLSNDFFANLLDMDTEWKPTSEARELFEGRDRKTGKVKWTATRVDLVFGSNS
                     QLRALAEVYASADAKEKFVKDFVAAWTKVMNLDRFELKRG"
     gene            complement(19760..19903)
                     /locus_tag="VIG70_10295"
     CDS             complement(19760..19903)
                     /locus_tag="VIG70_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HEY8250986.1"
                     /translation="MLTRRQVLGGPPVEIASLIVHSLPGRRDGLRERLVELPGVEIHR
                     RRR"
     gene            complement(19925..21562)
                     /locus_tag="VIG70_10300"
     CDS             complement(19925..21562)
                     /locus_tag="VIG70_10300"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEY8250987.1"
                     /translation="MSSLPGMAASGAARGIALLRDPLLNKGTAFTERERDQLGLRGLL
                     PAHILSLKEQADRVLANLRGLPSDLDKYVALNALHDRNEALFFRVVCDNIDEIQPLIY
                     TPTVGLACQKFGHIFQRPRGLFIGANDRGRVAEILRNWPYPAKLIVVTDGERILGLGD
                     LGANGMGIPVGKLSLYAACAGVHPELCLPVMLDVGTDNEALAADPYYVGLRRKRVRGP
                     EYDALVEELVAAAQQVFPGVLIQFEDFANRNAFRLLKKYRERICTFNDDIQGTAAVAL
                     AGVLCALRASGGRLSAQVLLYLGAGEAATGIADLVVSAMMAEGTSEADARSRNWLFDS
                     QGLVVKGRGELAEHKRPYAHAHAPVAQFDAAIRALKPTGIIGVAATGGLFTPEVLETM
                     ARLNQRPIVFALSNPTSKAECTAEQAYRHTQGRALFACGSPFDPVTLGGRTFVPRQGN
                     NSYIFPGVGLGVIASGARRVTDEMFMAAARTLAALVTEADLAQGSLYPALPRIREVSA
                     QIAAAVARVAYERGLALGEAPRDLLAHVQKQMYDPRY"
     gene            21695..22366
                     /locus_tag="VIG70_10305"
     CDS             21695..22366
                     /locus_tag="VIG70_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8250988.1"
                     /translation="MRTLLVAACAALIPAGPAHAQTAERTGEQVVKAQCAKCHEQGLR
                     GAPKIGDRQAWAPRMKLGLDATVRSAIKGHGGMPARGGMADLTDTELRAAILHLFNPA
                     AAASAKLEAAPAAAPDHNKKVVGGVEIYLGLMPAGPKGLYHVNISLRDGASKALIKDA
                     VVEARVASTLGGTTKKLNATTINESVSYSNDFRMVADERYTLTVTVRLPKAAAPLEAK
                     FDLKR"
     gene            22416..23714
                     /locus_tag="VIG70_10310"
     CDS             22416..23714
                     /locus_tag="VIG70_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEY8250989.1"
                     /translation="MRMLELTAVASAIALISGVVHAADPAKINWSKVPTKTVTLFYPG
                     QTSYEWVRGSAHPGAQAVQGGEACATCHTGQEKAKGDKLVKGGPLEPAPVKGKNGWVD
                     LKVQAAYDASNAYFRFQWKTKNGYPGTEHQYLRFDGTEWKVYGYPKLDKVVQEGKQPG
                     IYEDRMTLMIDDGKVAGFAKQGCWLTCHDGQRDMQKVVSKEDANANALLSAIKKNDLR
                     KYLPATRADPADWKTGKSLEEIAKIKTEGGFVDLIQWRGHRSNAVGMADDGYVLEYRL
                     SDAGKDMFSGNADPKTHQPKLMWDPKKVGYKSITEKQLRKGQHFLIREKNAVPFDPNA
                     GWKEGDMIPDYVVSREDAKGSAADNNATASWKTGTWTVVITRPLNLANEDDKALKEGG
                     VYNVGFAVHDDNITTRGHQVSFPVTVGFGAKAAIEATKIK"
     gene            23729..25858
                     /locus_tag="VIG70_10315"
     CDS             23729..25858
                     /locus_tag="VIG70_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY8250990.1"
                     /translation="MRPGGLLLAVAGLLFAGAASAQSGEALLEAKGCLGCHGIEEKQM
                     GPALREAAAKHKGAPAKLIAALREGKGHPVKVEATEPELKAMIGFLLAAQPSGAKPAA
                     AAAGSGNATCLACHGNEKFTPHLEQERFEKSVHGKRQCVECHKDITEIPHKKVAHKVS
                     CVTCHDDLWKAAQKEGKTQEHARLGVVVEQIDRYMRSIHARPNREDQSRTNATCYNCH
                     EAHYVYPLGSEQRAEWCLSIPKACGACHAKQLEAYATSVHGRETLQKGNPATAVCSDC
                     HTTHDIDRPTKESMKLAITKNCGTCHAASYESYRDTYHGQVHTLGYAYTAKCYDCHGS
                     HTVQRVSDPSSSVHPDNRLKTCQRCHAGATAGFVSFQPHATTDDFHRFPQLWLSAKFM
                     IGLLAGTFAFFWTHTALWFFREYRERQSRTPRPHVMTHEMPQAQQKHYRRFTAIWRLA
                     HLLFAVSLMVLTLTGMAVLYADSAWAPVVINALGGPQSAALIHRACAVIFAAVFLAHL
                     VYILNRVRKWRTLAQWFGPHSLIPNWQDLKDIYAMFKWFVGRGPRPVFDRWTYWEKFD
                     YWAPFWGVTIIGVSGLMMWFPAATASVLPGWVFNVATLVHGEEALLAALFLFTVHFFN
                     NHFRPDKFPLDVVMFTGTVPVEEFRREHTLEYDRLVQTGELQKYLVDAPSQPMTLGSK
                     ILGFTLIAFGLALLLLVLIGFSGANGP"
     gene            25861..27063
                     /locus_tag="VIG70_10320"
     CDS             25861..27063
                     /locus_tag="VIG70_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8250991.1"
                     /translation="MRLAPALLLIAFAMTPGSAAAQPARTKPAAGDDLRALYVNAPDI
                     AEGKRVAESSCVRCHGANGISPAKGTPHIAGQRAAYLYAQLRAYQQRPPTQSPMAGAI
                     RFLKDDALVKVAAYYAGLEPPRPSGGKAAAVEDPLQAGKAAAAACSSCHGESGVTAIP
                     GMPSLAGLDPKSFVAAMDAYKRGERPHDMMKSFAAPLSEAALNNLALYYALLKPERAR
                     TPAAGDAAAGKKAAAACGGCHGETGVSSIPGTPSLAGQDAQYLAAATNAYKDGTRKDE
                     TMKGPAAALDARAVQDVAAFYAAQQPQPPNVRKPLSLAEWIERCDRCHGPNGNSIEPL
                     MPALAAQRADWLEIVLAAYRSGARKSSAMGAMSASLSESDIRALAAHYSRQAARPVVY
                     VLVPEKRP"
     gene            27060..27827
                     /locus_tag="VIG70_10325"
     CDS             27060..27827
                     /locus_tag="VIG70_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8250992.1"
                     /translation="MNDTPLHREFRHFSEMPLSLRVLYTATLLVLGMGYLFALIYLFH
                     TYSGKDGNPLDLSYDDIVIAYTGSGKSSRLESALRGSMSVMLPKEELAPLVAWVHEGA
                     ERAAFEKQIRPTLEKRCLACHDGSNPHLANLNGFDNVKKVTERDTGTGIFTLVRVSHI
                     HLFGLTFVFFIVGSIFSHAYVRPVWLKCAVMILPFFALTLDVSSWYFTKLYQPFALVV
                     MAGGGLMGASFAFMWAVSMYQMWFSRTPAAVMHRSQQ"
     gene            27824..28951
                     /locus_tag="VIG70_10330"
     CDS             27824..28951
                     /locus_tag="VIG70_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8250993.1"
                     /translation="MKRLALGLAVLAVLAGCDGKGPAATQPQAGNVEAGKAFAERECK
                     GCHGLDGKGIAPAIPHLAAQRSRYLLAALTAYREGRRTHAALKEIAAHMTEADARNLA
                     AYYAALPPVPAGASQAAQRLSPYEQGKALAAACAKCHGDDGNSKTPGIPGLAGQQPHY
                     LVIAVQEYLNKERKAAPMHALLPGLRKRDVENLALYFASQTPAPRGAPPVGDPAAGEP
                     LSAVCGGCHGSHGVSLDASTPILAGQDARYLAGAIKAYRTSREREMMRRYVVGLSEKD
                     ILNIAAFYSVQKSRPAEKGQTLVQDLRDKCERCHGPQAADNPEMAIPKIAGQDRDYLV
                     MALRAYRDDRRESSMMHKMSLPYSDSIIESLASVYASQPAK"
     gene            complement(28952..29230)
                     /locus_tag="VIG70_10335"
     CDS             complement(28952..29230)
                     /locus_tag="VIG70_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8250994.1"
                     /translation="MRVALVALAVTGFLAAGLAQAQDLKAKGCLNCHESDKKKVGPAL
                     KDIAAKYKGDKGAEAALTAKLKEGKGHPKIAGSDAEIQALVKAVLATK"
     gene            complement(29262..31364)
                     /locus_tag="VIG70_10340"
     CDS             complement(29262..31364)
                     /locus_tag="VIG70_10340"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HEY8250995.1"
                     /translation="MKRTLLSVVVANAFIAAPAFAQEAMEWSGSVSAGVRNVDSDAQD
                     PSKLNEYRDLQEGNSGIFGFELRGRSDQNYFNAYGENLGRDDQYLDLNGGRYNTFKYR
                     LYSDQLQHNFGSGPGALSPFSGIGGATLTAPSPLPAVPLWGSFDHSYKRRDTGGMFEL
                     QKTSPWYFRVEANEVRRDGINVYGGANGTSPGNGFTDLPSPIDWLTRNYSAEVGYSTR
                     RAHFAVNLSHSTFENENELLRWSNRFFANGLDTTVLAPDNELTRLSVNGNLRQLALDS
                     TLAGRFTYSKLTNDVSLQPTMLSTGGVNAATNPSDPSFKGDIVKTTFGLSLSSRPTQT
                     VDTKLYFNWIKEENDSTHISFVNPGTGLTGASCTAADCTPELFEYEKRQYGAEAGWRV
                     TRANKLTGGLEWTDIERERADFQENEETRLFAEWKNSSLDWLTARLKYQLLQRRGDFN
                     PHPTLLAANPMDLFVRRFDVANVDQSLVKLALDFAPAPLLDLGLEAIYKENQYKDTPL
                     GRTEDTRHEVYASVGYGDPKSWRVHLFGDIEYVKFDSAHRVGTGNPDPATPPNAGTYN
                     WGSKNEDNSWQIGIGADWAVLTRLTLKASLIYAETDGHTDFSVQPGGDPTPRPSIVAS
                     DDTTRVAFNLRGVYALDRHWEFTAGYAYEKYRYSDIAYDGTRYVVGTGTQSSIVTGQF
                     SFQNYEANIFYGVVKYKF"
     gene            complement(31374..32354)
                     /locus_tag="VIG70_10345"
     CDS             complement(31374..32354)
                     /locus_tag="VIG70_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HEY8250996.1"
                     /translation="MASRTGFIALRLLTAVAAGLLLGIAPASAQAPAQRKPAPEAAVC
                     QNCHADYVESYLKTKHGQGGNLKGPDCQTCHGNADAHVAAGGGKGVGGILGFNSKALP
                     AERKAAVCLSCHQGDRHLAFWDSGAHRKQDVACSNCHSLHGTPGPGSTIALKKPNSTI
                     SPFVTTERQLEYETCTSCHRQIRSQLTKPSHHPIIEGKVTCSNCHNPHGALSKAMVKA
                     ESIPQLCTTCHAEKRGPFVWGHPPVEENCLTCHNSHGSNHARLLAEKAPNVCQDCHDA
                     SRHPGTIYDGSGGWNAATPNTRLIARGCVNCHYHIHGSNAPAMRGKFFLR"
     gene            32608..33264
                     /locus_tag="VIG70_10350"
     CDS             32608..33264
                     /locus_tag="VIG70_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8250997.1"
                     /translation="MIANLALFSGVAPGHVSTVARQSWALPVPRGAVIARRGSRLEGA
                     FALAYGFVELALRDAHGVERGLRFVAPGQTFGEATALLGRACKYEAIALAESKLIVIP
                     AAALFALMDHDARFARTLLMTLAQRELDLVAEIESATLHPAEQRLAGYLHSLADPGDC
                     PAACTVRLPVSKTAIASRLDMKKETLSRLLRALVQRGLIAVKQREITLLDRARLKQIC
                     "
     gene            33264..34004
                     /locus_tag="VIG70_10355"
     CDS             33264..34004
                     /locus_tag="VIG70_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8250998.1"
                     /translation="MMPSCSARARDLALVTQVIGKLALFTGLPPAILADVARQSWAFA
                     AARGTTFAARGALLPGIFALACGSARLTLRAAHNGERVLRLVSSGHTFGESAALLGRA
                     SSYDAIALADCTVVVVPAAQVLSLLERDARFARQLVTALAETKFGLLSELETATLPRG
                     AQRLAHYLASLAEPAELPGQFVARLPASKTLIASRLDMKKETLSRLLRSLAAQGVILV
                     KGREISILDRERLARIAPLNGEPHPLLT"
     gene            34064..34807
                     /locus_tag="VIG70_10360"
     CDS             34064..34807
                     /locus_tag="VIG70_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY8250999.1"
                     /translation="MRVGLFIPCYVDAFFPKVGIATLELLERFGVDVDYPLDQTCCGQ
                     PMANAGCQDDAAATERLFVRNFDGYDYVVTPSGSCAHHVREHLTAIGQTAKVKKVRRR
                     TYELVEFLHDVLRVRDFPWAKFPHKVGLHIGCATLRGLGAAKMSEIEGPPFSKPLTLL
                     SRVKGISVVVPSRPDECCGFGGTFSVFEEPVSARMGYDKVNDHKNAGAKYIVSQDMSC
                     LMHQKGCAERLGLGIRFIHIAQVLNGTAA"
     gene            34804..36183
                     /locus_tag="VIG70_10365"
     CDS             34804..36183
                     /locus_tag="VIG70_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HEY8251000.1"
                     /translation="MKRVDHAEASSRFIAAKEHVEFHDKRLWDLRQKRDREAAAIPEW
                     EQLRTLASTIKEHTLARLPDYLLQFERNATASGMRVHWARDAAEHNQVVHELLSAHGA
                     RILVKSKSMLTEECEMRPFLETRGIEVVETDLGERIQQLDDEPPSHIVVPAVHKLRTD
                     VARVFARTLGTDAGNSDVPYLAESQRRATRPYYLEARAGMTGANFAVAETGSFVVCTN
                     EGNADLSANLPKLHIASIGIEKMIPRIADLGVFIRLLSRSALGSPITQVTSHFRAPRP
                     GSEIHVILVDNGRSERLGMKDFWYSLKCIRCGACMNTCPVYRRSGGLSYGATYSGPIG
                     VIIDPAFNLRKYSALPFASTMNGSCSNVCPVKINIHEQIYKWRQIINERNQLPIVKKE
                     AMRMAGKVLSSPKLYRAAVKAANAGLEYLPRSMIYNPLNAWGRSREVPKAASASFRDW
                     YVKHRGAGK"
     gene            36180..36755
                     /locus_tag="VIG70_10370"
     CDS             36180..36755
                     /locus_tag="VIG70_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEY8251001.1"
                     /translation="MSARDDILARLGRNLPLCPPLPEIPLFDSSSPDLKEKFRENLAR
                     MGGELIELSPSRIPAWIRGKFPDGAVICSATPEVAGNRPLESMRDPRELESVDLGVVR
                     ARFGVAETGSVWLSEAEFKVNALGFLPQHLLVLLDLRKLVPNLHHAYRDPGFLEARYA
                     VLMTGPSATADIEGVLVRGAQGVRSLTVVGC"
     gene            36799..37368
                     /locus_tag="VIG70_10375"
     CDS             36799..37368
                     /locus_tag="VIG70_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY8251002.1"
                     /translation="MFQIRIHGRGGQGVVSAAEMLSVAAFLEGKHAQAFPSFGSERTG
                     APVVAFCRIDESEIRLREPIMRPDALIIQDPTLLHQVDVFAGLAPGGTVLINTARSFA
                     DLGLQDFIEKFKVCALPATELALKHVGRPLPNAALLGGFSALSGRLALGSVKAAIREK
                     FSGKVGEGNVAAAGAAHEYVLKELHAEAA"
     gene            37352..>37726
                     /gene="porA"
                     /locus_tag="VIG70_10380"
     CDS             37352..>37726
                     /gene="porA"
                     /locus_tag="VIG70_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEY8251003.1"
                     /translation="MLKQLEGSHAVAEAVALCRPEVICAYPISPQTHIVEGLGEIVKS
                     GALSPCEFINVESEFAAMSVAIGASAAGARAYTATASQGLLFMAEALFNASGLGLPIV
                     MTVANRAIGAPINIWNDHTDSMS"
BASE COUNT         6007 a        12998 c        12705 g         6016 t
ORIGIN      
        1 gaggcgggcg gaatctcgat cgggctcaac atccggctgc cgcgcgagca agtcgcaaac
       61 cgctacatca cgccgggctt gtgcttcagc ttccgctact tcgcgctcag gaagctgcat
      121 tttcttctgc gcgcgcgcgc catggtggcc tttcccggcg gcttcggcac cttcgacgag
      181 ctgttcgaga ccctcaccct cgtgcagtgc cgcaagatcg cgccgatgcc gatcgtgctg
      241 gtcggcaagg cttattggcg gcgcgcggtg gacatggagt acctggccgc gaaaggaatg
      301 atcgcgcagc gcgaccggcg gctgtttcgc tacgccgaga ccgcgggcga gatctggaag
      361 gccgtggcgc ccaacctggc aacgacctag gcgcgaagcc gcagcaccgg agcgggccgg
      421 ccgcgcacga agggcggctt cgcctgcagg tcgatcctgg tcatccattc acagccggcg
      481 cacagatcga cctccacgtc gcgcccgcgt cgcggacagc tcaccgagcc gcggtccacg
      541 taatgctcgc cgccagggga tcgaaataag gaaaacatat cagcctcctt ggaaggtcag
      601 gtccgcacga gaagcacggg cacggacgcg ctgcgcacca cctgctcggc atcgctgccg
      661 agcaccaggc ggcgcacgcc gcgccggccg tgggtgccca gaacgatgag atcggcacgc
      721 agcttcctcg cctcgcgcac gatcgggtag gccgccgggc cgctcagcat ttcccgcagg
      781 atgcctttgg gtttgatttt ctgtttcagt gcaatcccca cggctttcgc gaggatgcgc
      841 ttgccgcctt cgcgcagcgc ggacagcagg tcggcgcccg gcgccccgac caggccgtcc
      901 atggcggcga aggcctggaa ctcgtcgacc acgtgcagga tcaagagctt cgcgcgctcg
      961 gcgcgcgcga ggcggatcgc ctcgcgcagg cctttgctcg aggccgggct gccgtccacc
     1021 gcaaccagaa tcttgcggta ggcgcccatc acgaaaccgc cacgcgcttg gccgagccgt
     1081 tgcccttctt cggcagcgtg aggttgagca cgccgtcccg gtactcggct ttcgcggctt
     1141 tcgcgtccac gtcgaccggc agcgtgaagc tgcggctcac catgccgtag gctcgctcgc
     1201 tgtagacgac tttctcgtcc ttcttctctt ccttctcctg cttcacttca gcgcgcagcg
     1261 agatgtggtc gtcctcgatg tcgatctgga tgtcttcctt cttcacgccg gggatgtcgg
     1321 ctttcaccgt gaagctcttg tcgtcttcct tgacgtcgat cttcatcttc agctcggtct
     1381 cggcgggaaa cggcagcggc ttcagccaga agcccttgcc gaagtcctgg aaaaagtcct
     1441 cgaatgggtt gtagcgcgtg atgttggcca taaggccctc ctttggcgag tcagtcggtc
     1501 aagccatttc ttcctcgcgc gcccgggcgg gcttgtccgg gatctgcgcg atcatgcaat
     1561 cggtggtgag gatcaggctc gcgatcgagg ccgcgttctg cagcgcggtg cgcgtcactt
     1621 tgcgcgggtc gagcacgccc atcgcgacca tgtcgccgta cacgccggtc gcggcgttgt
     1681 agccgaagtt gccctcgcca tcgagcacgc gctgcaccac caccgccggc tctgcgccgg
     1741 cgttgctcac gatctggcgc agcggctctt ccaccgcgcg ctcgacgatg cggatgccgg
     1801 cgtcctgatc cgggttttct ccttgcagac tagccagacg ttttctggcc ctgaggagcg
     1861 cgaccccgcc gccgggcagg atgccctcct ccaccgcggc gcgcgtggcg tgcagcgcgt
     1921 cctcggtgcg gctcttcctt tccttcatct cggtctcggt cgccgcgccg acgcggatca
     1981 gcgccacgcc gccggcgagc ttggccgcgc gctcctgcag cttttccttg tcgtagtcgc
     2041 tggtcgcttc ctcgatctcg gccttgatct gcgcgacgcg cgcatcgatg ttcttcctct
     2101 cgccggcgcc gccgatgatc gtggtgtcgt ccttgtcgat ctcgacgcgg cgcgcgcgcc
     2161 cgagcaccgc cgggtcgacc ttctccagcg tgatgccggc ctcctcggcg accaccgtgc
     2221 cgccggtgag caccgccagg tcctcgagca tgctctttcg gcggtcgccg aagcccggcg
     2281 ccttcaccgc gcacgagacc agcacgccgc gcagcttgtt caccaccagc gtggcgagcg
     2341 cctcgccctc cacttcctcg gcgatgacga ggagcggctt gccggtcttg gcgacgtgct
     2401 cgagcaccgg caagagctcg cggatggtgg cgatcttctt gtcgtgcagc aggatgtacg
     2461 cgtcctcgag caccacgctc tgcttctccg cgttggtgat gaaatacggc gacaggaagc
     2521 cgcggtcgaa ctgcgtgccc tcgaccacct cgagctcgtt ggcgagcccc ttgccgtcct
     2581 cgaccgtgat cacgccctcg cgcccgacct tctccatcgc ctcggcgatc atggcgccga
     2641 tcgaagcgtc gttgttggcc gagatcgcgc ccacctgggc gatctcgttg cgagtcgagc
     2701 acggcttcgc catgcgcgcg agctcggcga tcagcgcctc gaccgcgctg tcgatgccgc
     2761 gcttcaagtc catcggattc atgccggcgg cgacgtactt catgccctcg ttgacgatcg
     2821 ccgcggcaag cagcgtcgcg gtggtggtgc tgtcgccggc cacctcggag gtcttggcgg
     2881 cgacctcgcg caccatctgg gcgcccatgt tctcgaagcg gtcctcgagc tcgatttcct
     2941 tcgccaccac cacgccggag ttgatcacaa ttggcgcgcc ccaggatttc tccagcacca
     3001 ccgtgcgcgc cttgggcccg agcgttacgc gcaccgcgtc ggtgagcgtg ttcattccct
     3061 tgatgaggcg cgcgtgcgcc gactcgccga acagaatctg ctttgccgtc atgagccgga
     3121 cttttcaaaa ggatggtaga agcgctgcac cgtatcgagc cactgctggt aagcgccgtc
     3181 gatcatctcc tgcttgagca tcccggaggc cgcctgcggc gtgccggcct gcagaagcga
     3241 gcgccagtag gcgaacgact tcccggcatt gctgcgcgcc cattcggcgc agagcttggt
     3301 gagctgcgcc gggtcggtcg ccgcggcgat gactgctcga gtggcttcgg cgtcggcatg
     3361 cgcctgggtc gcggccgaga gctgcacttc gtgcatccgg atcgcgctct cggtgatggt
     3421 ctcgatcgcg cgcagcccgg cgtcgagttg cgccttccag gcccgcatga acatctcttc
     3481 gggtttcgcg ttcatagtgt cctcgtcagc cggggccggc gcattcatcc cggcgctcca
     3541 ccgcgccgag ctgcctgcgg tgacgttctt cgcagcgcgg atgctgcagc acgaaattgc
     3601 gcagcacgag cttcagcgcc agctcctgca ggcggtcgcc ctgggcgcgc gcctcgtcga
     3661 tgtcgtcgcg gatgatggtc ttgatgcgca tctcgccgcc gggcgagtgc acgaggtagt
     3721 tgccgagctc cacggcggcg atttcaggga tgcgctcgtg gtgcgcgatc gcgagcactt
     3781 cctcctcgct caggtcgcac agcgcgaggc agtcttccag gctaagcatg ggaggtcagg
     3841 gaacgaacgg cggcgtcgaa cgccgcctgc accgcctgct ccgcgctcgg acgcgccggg
     3901 ccgctgatga ggcattggtg ctgcggccag cgcacatcga tccgcaggga ccagcgcggc
     3961 tcggcggcct cgcgccagtc ctccagcttc gcgctgcagg cgctgatgcg cggataagcc
     4021 gcgcgcagat cggcgatgcg gtgctcgatc cctgcctgaa ggctgtcgtt cttcattctt
     4081 cctcggtcag ccaccagggc gcgaactggc gcaactgctt gcgctgctcg ggcgtgagaa
     4141 ccgccgcgat ctggttgtgc gcgtcgacgc gcgacttgag catctgctgc cgcagctcgt
     4201 cgaccttttt ctgctgcgcc acgagcttgt cggcgtcgag cttctcggtg ccgtacatgc
     4261 cgcgcagctt gaactgctcg gagcgcaccg cgcccatcgt cgcccagttc ttctggcgct
     4321 gcgcttcctg gatcgcggag atcttctcgc gctgctcgtc gctcaggttg agcgcggcga
     4381 acatcccgcc cgcgccgtag ccgcccattc cggggccgcc catcattccg ggaccaccca
     4441 tcattccggg gccgtagccg cccattccgg ggccgcccat cattcccgcg ccgtagccgc
     4501 cgcccatcat cccgcgcccc tggccgtagc catagcccgg gccgtagccc tgcgcggccg
     4561 cgagcgagct cgcgagggca agcgccgcgc cgatcatcag catctttctc attgccgtct
     4621 ccttggaaag gttctgacac cagactagcc cgctggccgc ggcgcccttt gatcccggtc
     4681 aaaggtcgac gcggtcggtt gcgtacgatg acccgacaag gaggccacca tgcctgcagc
     4741 gaagaggaac atcggcgacg agatgcgcgg catcacgcgc aaggccctcg agcgcggcac
     4801 gctcgaccgc gctgcggtgc gcaaggtcac cggcgaagtg cgcaagcgcc tgctgcagat
     4861 cgagcgcgtg ttcgcgcagg acctgcgggt cgccgcgcgc gcgggcaagg acgccgcggc
     4921 gcagagcatg agcgcgacgc tcgtttccgg cgccatcgcc gcgcgcatcg cctcgggcat
     4981 gctcgccggg atcgccgaca gcatcgcgcg cggcaccgcg cgccggaccc cgcgccggcg
     5041 gcgctgatgc tgcgcgcgct cgccggcacc gcgcgcgacc tcggccggct gcaggagatc
     5101 ggcgcggtcc tgctgcgcta cggcttcggc gacctggtgg ggcgtctggg catcatcctg
     5161 cactggaagg actccgacca acccgcgcag ctcggcgccc cgcagcgcgc gcgcctcgcg
     5221 ctgcaggagc tcgggccggc gttcgtcaag ctcggccaga tcctcgccac gcgcgtggat
     5281 ctgttcccgc cggagtggat cgcgcaattc gaaaagctcc aggaccaggc gccgcccgcg
     5341 gcagcgcacg aggtgcgggc ccagctcgtg gaggacctcg gcacggcgcc ggaagaggcg
     5401 ttcgcggcct tcgatcccga gccggtcgcc gccgggtcga tcgcgcaggt gcaccgcgcg
     5461 cggctcgccg acggcaccgc agtcgcggtc aaggtgcgca ggccgggcat cgtgccggtg
     5521 gtcgaggccg acctgcggct cctcgcccgg ttcgcggaga tcctcgaagc gcgctttccc
     5581 gatgtcgccc gcttccggcc gcgcgaggtg gtgcgccagt tcacgcgcac cctgcgcacc
     5641 gagctcgacc tcgccgccga atgccgcaac gccgagcgcg tcgcgcagaa tttccgcgcg
     5701 cacccggaaa tcgtcgtccc cgcggtgtac tgggcctgga ccggcgagcg ggttaatgtg
     5761 caggcgttcg tcgacggcac gcccagccgc gaccccgagg cgctggaggc cgccggcctc
     5821 gaccgccacg cgctcgcgca ccgcggcgcg caggccatgc tcaagatggt gctcgaggac
     5881 ggcttcttcc acgccgaccc gcaccccggg aatgtgttct acctgccggg cgaccgcgtc
     5941 gccttcatcg acttcggcat ggcggggcac ctttccgagc gccggcgcga cgagctggtg
     6001 cagctcctga acggcctggt cgggcgcgac gccgaagccg tggtcgagat cctgctcgac
     6061 tgggccgggg cggcacgggt cgatgccgag aaagtgtcgc tcgacgtcga cgccttcatc
     6121 gaccgctacc acggcctgcc gctgcacgag ctgcgcatgg gcgcgatgct cgccgacatg
     6181 accgcgctct tgcgcgagca ccagctcgcc ctgccgcccg acctcgcgct ggtcctcaag
     6241 gcctgcgtga cgctcgagac ctacgggcgc gagctcgatc cgcaattcga catggtgggc
     6301 gaagccgcgc ccttcctgca ggcggcgacg ctcgagcgct tcggtccggc gcgcctggcg
     6361 aagcgcggct ggcgcgcggg ccgcatgctg ctggaggtgc tgtcggacct gccgcgggac
     6421 ctgcgcgagc tcctgcgctc cgcgaagacc ggcaagctgc aggtgcacgt cgacctcacc
     6481 agcctgcacc gcttcggcgt ccagctcgac ggcgcggcga accggctcgc gatcggcatc
     6541 gtcaccgcgg cgttcatcgt cggctcgtcg atcgtcatga cggtggaggg cgggccgacg
     6601 ttcttcggcc tgccgctcct cggcgcgctc ggcttcagcg gcgctctggt cggcggcgcc
     6661 tggctcttcg tctcgatcct gcgcagcggc cgttaacccg gcctgaaaac caggaagtac
     6721 tggttcggca ggaagccgtg ctccccggcg agcgcgtagc cggcgctttt catctccgct
     6781 ttcacgcgct ccggagcgat gcgcgcctcc ttgggaggcc cgttgggcga atcgaggcga
     6841 aagtcgatca ccgcgaggcg gccgccgggc ttgagcgacg cggcgaggct gcggaaatag
     6901 cggccgcggt cctcgatgtg gtggtagacg tccacgagca ggatcaggtc cgcctttgcg
     6961 ggcaggcgcg gatccccggg gaggcccgcg accggcttga ggttccccag tccttcgcgc
     7021 tgcgcccgct cgccgaggta cttcaccatc gcgggctcga tgtcgaccgc gtagacggtt
     7081 cccttcggca gcatgttggc caggcgcgcc gcgaagtaac cggtgccggc gccgatgtcg
     7141 gcgacgatcg cgtcgggctc gagcgcgagc gcccggatca cctggtgcgg cttctgccag
     7201 gcgtcgcgct cgggatcgtc gaaaacatgc gcccactttt cggcgtcgcc gaaaccgtgg
     7261 cgatgggtgt gcggcgcctg cgccgcggcg ctcgcggccg ccagcgccgc ggcgaacacg
     7321 acgcacctag agacggtgaa ggacgttctc aactgagcgg gctcacttgt gccatccgta
     7381 tcctaactcc cgcttgatcg aaatcaatac gcggccgcgc gaagcgagga tgcttgaggc
     7441 tcgcaaagga ggtacccatg acgcgttatc tccccgtcct cgccctggca gtgttttccg
     7501 ccggcgcggc cgcacagccc tttcccatga acgagcacgg cgacgtgcgc tgggtctgcg
     7561 ccggtatcgg cgaaggcgag cgcgaggcgc tcgccaagat ggaggccggg acgagcctca
     7621 agctcgtgtt cacctccggc aagcgcggcg aataccttac cgacgtcgac gtcgtgctga
     7681 gcgaccgcga gggcaagcgc ccggcactga agttcattgc cgaagggccg atttgcctga
     7741 tccaggcgcc cgcaggccgc tatcaggtcg aggccgcgtt cggcgacacg aagcgcagcg
     7801 tcgccaccac ggtgacgaag ggcgtcaagc agcccgggat ggtggtgttt cgcttccccg
     7861 gaacgcaata ggccccgccg gcttgcgcgc gaccaggtcg acgagcgcgg agcggcccga
     7921 gtgctgcgtg ccctcggcga gcaccgcctc gtaggccttg agctcgagga tctcgagggc
     7981 ggcgaacgcc tgccgcagca tctgcggcgt gtagaggttt tcgagatgcg gcgggccgcc
     8041 ggtcctgtac tcgagttgct tgggcgcata accctccagg agaagcagcc cgcccggctt
     8101 cagggcgcgc acgatgcgct cgaacaggaa gccgcgcatc gccgggtcgg cgaactggac
     8161 gaagatcgcg gcgaccaggt cgaacccggc gtgcggccag cgccagccgt agacgtccgc
     8221 cacttcgaat gcaaccttga cgccgcgctt ctccgcgagg cgacgcgcct tctccacgcc
     8281 caccggcgag agatcgaacg ccacgacttc gagcccgcgc tcggcaagcc atacgctgtt
     8341 gcggccttcg ccgtcggcga cgcacagcgc gcggcttcca ggcaccagca gcgcggcctt
     8401 ggaggcgagg aattcgttcg gcgcggtgcc gaagatatag tcgggcaacc cgaagcgggt
     8461 gttccagcgc tctagctcgc ccatcgcacc agcgctgcgg catcgagcgc cccgctgacg
     8521 cgcttcactt cgaccccgtt gcggaacagc gcgatcgtcg ggatcgcgcg gatgcccatg
     8581 cgagccgcga gcccgggggc ttcctcggta ttgaccttga ccaggcgcgc gcggcccttc
     8641 agctcgcgcg ccgcgcgctc gaagtgcggc gccatggcgc ggcacggtcc gcaccacggc
     8701 gcccagaagt cggcgatcac cggcaactcg gtgcgacgga cgaaatcgtc gaactgcgcc
     8761 tcggccagct cgagcggcgc ctccggcagc agcgccgcgc cgcacttgcc gcacttcggc
     8821 tcctcgccga gccgcgccgc cggcacgcgg ttggcggtga ggcaggcggg acacgcgacc
     8881 tggatggggt cagagggcat tgagcgggat cttcggtttc atggctaaca atatatttac
     8941 tgatatattg tttgtcaata gaatgtcagt catgatcgaa ctcgactcca tgcacgaggc
     9001 ggcggccgag gcctgccgcc tgatgaaggc gctcgccaac cccgaccgcc tgatgctgct
     9061 gtgccagctg tcgcagggcg agtgcagcgt tggcgagctc gaggccgcgc tcggcatcct
     9121 gcagccgacg ctgtcgcagc agctcggcgt gctgcgcgag gaggaaatcg tgcagacgcg
     9181 gcgcgaaggc aagcacatct actaccgcgt ccacagcccg caggcgctcg ccgtgctgca
     9241 ggtgctctac gaccagttct gccggaaagg aaggaaacga tgactctcga ctggacccac
     9301 tttaccccct gggcgtcggc cgccggcggc gccctcatcg gcctcgccgc ggcgatgctg
     9361 gtgctgctga acggccgcat cgccggcatc agcggcatcg tcggcggact gctcgtgccg
     9421 cgcggcggcg acatcggctg gcgcctcgcc ttcgtcggcg ggctgctggt cgccccggtg
     9481 gccatgtctt tctacgaggt gaatccccgc atcgacgccg ggttcggcac cctcgcgctc
     9541 gccggcgtgc tggtcggcat cggcacccgc tacggctcgg gctgcaccag cggccacggc
     9601 gtgtgcggcc tgtcgcgcct ctcgccgcgc tcgctcgtcg ccacggtcgc gttcatggcg
     9661 gcaggcgtcg ccaccgtctt catcgtgagg cacctggtat gaacctgctc gcggcgttcg
     9721 gctccggcct ggtgttcggc atcggcctca tcctctcggg catgaccgat ccgggcaagg
     9781 tgatcggctt cctcgacctc gccggcaact gggatccttc gctcgccttc gtcatgggcg
     9841 gcgcgatcct ggtcggtttc ttcgccttcg ccctcgcggg ccggcgcagc aagactttcc
     9901 tcggcgctgc cctgcacctg ccgcagcgcc gcgacatcga caaccgcctg gtcggcggca
     9961 gcctgctgtt cggcatcggc tggggtctcg ccggcttctg tcccgggccc gccctggttt
    10021 cgttcgcctc cggcgtcgac caggccgcgg tgttcgtcgc cgcgatgctc ggcggcatgc
    10081 tcgtctacca tgccgcggag cgcctgatcc acgagccgcg ccggcgcgcg acgtgaatgg
    10141 cttcccggga gcctcgccta cgcgatgctc gcggggctgc tgccgcaggc cggcctgtac
    10201 gcgagcatcc tgccgctcat cgcgtacgct gcgctcggcc gtggaaggtc agctgcaccg
    10261 tttcattgct ttttcttcgg gggaagtccg atgcggtaat acgattcgtt gaggtactgg
    10321 accatgtcct cgagctcctc gagcgaatag acgcgctgct tggtgtgcgg cgcccattgc
    10381 gccaccatat cgcgcaggtc ggcgaggtcg cggaccgccg ggcgcaggcg ctcgatgtgg
    10441 atgcgctcgt agtggcagcc gccgcagtag gtctggtaga gcacgcggcc gcgctccgcg
    10501 tcctgtgcga atgcgcaaag cggaagcagc aggatcagca ggctaacggc tcgcatgttt
    10561 ttgctccaga aagtgagtga gccagctcga gccggccgcg atggcccaga acaggacgaa
    10621 gccgacggtg tacccggcct tgcgcccgaa ctcgaacggg cctacctcgg tccggcgtga
    10681 gcggcaggct catcgcttga gcgccgccag cggcatcgcc gccttgccga gaaacgcaat
    10741 cagcttggcc atgacttcgc catgatggcc gacccagaga tggccgccac gcccgtaacc
    10801 gatgaaccgc gcgccgggta tgtgtgccgc ggtatagctg gcgctctcga atgtcccgta
    10861 cgagtcgtct acgaggctca cgatcagcgt gggcgtggaa atgcgctcga gatcgtagcg
    10921 cggcagcgac gcggcgatcc gggcctcgtt cgtcagtccc ggctgacgcg cgctgagcgg
    10981 caggatctgc agcatcagct tgcgcacccg cgcctgctcg tctgcgcttg ccgtcgccag
    11041 cacctcgggc ggcgtcgcca ggaccgtctt cacgatgagc gacggggcgc ccttcaatgc
    11101 cgcccagtag agcaggtccg acttggtggc ctgctcgatc atgaagcgcg ccgcccgcga
    11161 gggcacggcg ggagctgcgc gcggcgcata ggcgagcggc acgagcagga ccatcgcgtc
    11221 cgttcggccc gggtagcgca gcgcgaactg catcgtcgac ggcgcgcccg ccgagatgcc
    11281 cacgatcgcg gcgcgctcga cgccgagcgc gtcgagcagg caggcgtgcg cgtccgcctg
    11341 cgcggcgggc gaggcgtccg ccggcagagg cgtgcgcaga tacccgaagc gcgacatcgc
    11401 gatgacctgc aagccatgcg cagccagctc ctgcgcgatc ccgagcccct ggtcgaagcc
    11461 gccgccggcg ccgtggacca ggagcaccgc cggcccgctg ccgagcgacg catactcgat
    11521 cggcccgcac cgcgtgtcgg cgatatggct gccggtggaa acgcgctcca cctcgcgtgc
    11581 cagatcgcgc tggtaggtga gccaggcgcc ggcgagcagc gcggtggtaa tgctgaaggc
    11641 gatcaaacgc gctttcatga cgtcgcgcag cgtcccgcca ccgtcaccga gcgattcgtg
    11701 cgctggacgc acgagccgtc ttcgatgcac ggcaggaacg cccagccgga ggggcacatc
    11761 ccgtgccact tcacgcgctc ggccacgaaa tggcggaact ccgggctgcg gatctcgccg
    11821 ccgagctgcc gcagctggtt cacgtgcacc tccatcacga actcggcaga gttgaccgcg
    11881 tcgatgctgt aacccgcgtc catcaggtac cagttgctgc tcggcgtcca ggcgcaggac
    11941 gcgaggagca tcacagcgag cgtcaggggg cgtttggccg acatgccctg atgctaggcg
    12001 cggcccgcgg gccaaccttg agctacatca atgtaccggc ctcggaattt tcctagcgca
    12061 cgatgaaagc cgcgcgctcg cgcacctcca ccccctcgac cgcagaccga tgacttgacc
    12121 tggcgcaaac cgccgccgcc aaggtggccg aagatccctc cgatgggaaa ccaaggtctt
    12181 gcgctgggca gtccgctgca cctcgcagtc atggtcgccg ctatgctcgc ggtgatggcc
    12241 gcggctatga tgctgctcca ctgaagaagc gcgtttcctt cgccaccgtc gccgtcaccg
    12301 gcctcggcgc cttcgtcgcc gcggccgtgg gcgtcacctt gtatgtgagc gccgccacgg
    12361 ggtggcgctc gacccaggcg ctgatcggac agcagaccga ggcgctgctc gacgcgctcg
    12421 agcagcgcct cgcggcgcgc ctgcgcccgg ttcacgagca ggccgactgg atcgccgagg
    12481 cggtcaacaa cgggcgcatc gatctcgcgc gtcccggcga gctcgacagc ttcatgttcg
    12541 gcgcgctcgg cgtcaccccc caggtatccg acatcggcat cgtggacgcc gcaggccggg
    12601 cacaacgatg gcggcgcggc gagctgcgcg cgctacgcga cgactggtcc tcgcgccccg
    12661 aggtacgcga atggcttgcc gcgggacagg tggagcccgg gcgggcgtgg cggccgctcc
    12721 tgtggaagcc ggccgagcgc attccctcgc tcctgcacca ggcgcccctg caccgcgccg
    12781 gtcagttcgt gggcatgctc gggcaggtgg tgccgatcgc gaaggtctcg gaggatctcg
    12841 ccgtgttccg cgccgaatac gaggtcacgc ccttcatgct cgatagcgag gatcgcgtgc
    12901 tcggccaccc ggcgctcgcc cagcggcgcg cggacgcgcc gggggcgttc ccgacgcttg
    12961 ccgagctcgg cgacccggtg ctcgcgcggc tgcgctcccc cgacggcgcc gcgcgactgc
    13021 tctccggctt gaagcgcgcg caggccacgc gcgctcgcgt cgatggaagg attttcgtct
    13081 acgtgtaccg cgagatggcg ccgcacggcg gacagccgtg gactctcggc ctctacctcg
    13141 acccggtcga gggcggccag cgcgacgaga tgctgcgcgt ggtctggtcg atcgctgccg
    13201 ggctggcagt cctcgtgatt gccgtaaccc tcgccgccgt cggtggccgg cgcctcgggc
    13261 ggccgatcga gtcgctggcg cgcgccgcgc acctggtgcg caagggccgg ctcggcgaag
    13321 tgcagccgct gccgcgcagc cgcatccacg agatcgacga ggcgaaccgc tcgttcggcg
    13381 agatggtcga gggcttgcga gagcgcaacc tgatccgcga cacgctcggc cagttcgtgc
    13441 ccgagcaggt ggcgcacgag ctgctcgccg gcggcggtcg gctcgagccg gtggaagcca
    13501 aggcgaccgt gctggtgtgc gacatcgagg gcttcgccgc gctcaccgac gagctcggcg
    13561 cgcgccgcac catcgagttc ctgaacgcgt atttcgaggt gctggttgcg atcgtcgagc
    13621 gctaccaggg cgtggtcacg cagttccagg gcgacgcgat cctggcggtg ttcaacgtgc
    13681 ccatcccggc ggccgaccat ggcgccaacg cgctgcgcgc ggcgctcgag atcgtgcgcg
    13741 cggccgactc gcaggcgttc gccggggtgc gcgcgcgcaa ccgcgtgggc ttgagcacgg
    13801 gacgggtggt ggcgggcgcg gtcggctccg ccggacgcct ctcctatacc gtgcacggca
    13861 acgcggtaaa cctggcggcg cggctcgagc aaatgaacaa ggagtacggc acgcgcatcc
    13921 tgctctcgga caagaccgcc gagcgctgcc ccgggttcgc gctgcgcaag gtggccgatg
    13981 ccgagatccg cggctacggc gagccggtac cgctctacac gcccggctag cgcttcaccg
    14041 ttttcagatc cgccggctcc tccatgattc gcatcggcat gaaggagagc ttgcgcgccg
    14101 ccagcctgcg atcgaggcgg gcgttgagct ccaccagcga gacgcggcgc ggatcgaacg
    14161 ccacggaaag cgtcagcgtg ggcagcgaaa gccgcacgct gccccgctcg acgcccggaa
    14221 ccgacgccgc cgcattctct agctcgcgct gggtggctgg cggcccgtcg atgtggaaga
    14281 acacgacgtg gtgtccttgc gccagggcgc gggtcaccac ggcgtggtcg taggtggagg
    14341 cgatcttgtc ctcgacgcag tagccgcacg cggcggcttc ggcggcgagg aatgcgagcg
    14401 ccagcacgag gcttcgagtc atgggattta gtgacccccc tgcgccttag ccgcacccgc
    14461 cggctgcgaa tcgatccact ggaagtactt gaggtactgc cggacctccg cgtcggtcag
    14521 gctctggttc ggcatcggga tgttgttgta ctccttgagc atcgccttcg ccgtcgcatc
    14581 ggtctgcagc atcttctccg gctccttcag ccagcgcgcg agccaggcct gctcgcgccg
    14641 cttggtgacg ccggcgacgt ccgggccgag cttgcgccct tcgcccaccg tgtggcaggc
    14701 gaggcacttg ctttcgaagg cgagcttgcc cgccaccgcg tcgggatcgg tcggggtcgc
    14761 cgaggcctga aggttgtggt gctccagatc cttggccggc ggcttggccg tggtggacac
    14821 gaggccgatc gcgccctgcg acgcattcgc gaagtgatga tcgaccatga tgtaggagcc
    14881 ttcctccgga atcaggtact cgacggtggc gctgttcgac gatccgagaa gcacggtctg
    14941 catgccgcgc agctggttgt cggggttgcc ctccatccag acgcggtcga agatcgtgcc
    15001 gacgacgtgg aagctcgagg tgcggctcgg gccgacgttg agcacgaaca tgcgcacgcg
    15061 ctcgccgggc ttcgcggcga gcggcgtcct caccatgccg ttgtgcgagc cgttgaacac
    15121 cgtgtgcgtg ggctgcgcgg cgcgcagccg ctcgctgtcg agcacgtaga gcggcgcgcc
    15181 gtcgatcttg cgcttctgcg gatcgggctt gaggtagaac tcactctgca ccacgacgta
    15241 ctcgcggtgg gccttggtcg gatagccctc gcgcggatcg acgatcaccg ccccgtacat
    15301 ccccgaagcg atgtgctcga ggatcatcgg ggtgccgcag tggtacatga agatcccggg
    15361 atagttggcg gtgaactcga actcgatcgt ctgccccggc gcgatcgagc ggtacttgtc
    15421 ctgcggcgag accattgcgg cgtggaaatc catcgagtgc atcatcggcg ccgccatcac
    15481 ctgcacgggt gccacggctt cgtcgctgcg gttggtcatc acgaagcgga tgcggtcgcc
    15541 gacgcgtgcg cgcaccgtcg gcccgggcac ctggtcgccg aaagtccagg cggcgaactt
    15601 caccccgggc gcgacctcga tgatcttgtg cgtggtgtcg aggcgcaccg tcttcaccgg
    15661 cgcggcgtct agcggcttca gctccgcccc catgctgagc gcggcgccct cggcgaacgc
    15721 gcctttgtga cgctccggcg tgaccttcgc cggcgcgggc ttgaggtcca tcttcgagac
    15781 gtcgtagcgg tccggcggcg ctgcgggatt gcaggcggcg agcgtgacca gggcgactgt
    15841 gctaagcagt tttttcacgg taggaactcc cttgaatcag taatcgtccg taatgcagag
    15901 cgaagagacc gaaggccgcg ctccaggcga gcccggcaag cgaagtgacg acgagggcct
    15961 ggccgcccga gagcgggctg agcacccgca ggagagcagc cagcgtgacg agcgcataga
    16021 tcgcgaccgt cgcgccgtcg gcgacgagcg cgcgcccgct gtggccgagg gaggcgcgcg
    16081 tcatcaccgc gagcgtcatg gttccgactg cgccgaccgt gagcgcgtgc agcgcagcgc
    16141 cgcccggcac gtcgaggccg agaaggatca gccctagggc gagccaggca taccccaggt
    16201 gcaggatgaa cagcagcggc tcgcgcaccg tggacaggcc gcaccagcgc gagagccgcg
    16261 ccgcgagccc gaccccggcg gccaccaggg gccacgtgat gcccgcagcc acccacgccc
    16321 ccagggcggc gagcgtgacc agaagcgcga ggtgatcgaa gcggtcttcc ggcgccggca
    16381 agcgcgcgcc gcggccaagc ttcgccagcc agttgcgcgt gaagctcggg acgatgcggc
    16441 cgccgaccag cgcgatcagc atcagcagcg tggcgagccc gagccgcagg ccgaggtaga
    16501 tctgcaggtg aaagaggacg ttgccggcga agaggaggag cagtgctacc agcatcggca
    16561 ggttgcgcca gttgcgcccg gcaaccagct cgcgcgccac cacggcgatc agcgccgcgg
    16621 taaacgcgag atcgatgaag gccgcttcgg gcacgagcat tgcgacgcgc ccggcgagcc
    16681 aaaggagcgc gagcagggcg agcggcgcgc cgcgcacggg caagcggccg gtccagttcg
    16741 gaatcgccgt cagcaggaac ccggccaccg aagcaagccc gaacccgtac accatctcgt
    16801 gcgcatgcca ggccatcggg gatacgccgc cgcgcagctc gtagccatgg gcgtacgcgg
    16861 cgagccacac cggcacggcg atcgcggccc agaccgaggc caggaggaag aacggacgga
    16921 atcccgcggc gaacaccagc cgacgataga caggcgggcc ctgcccggct tgacccagat
    16981 caaacgcggg gcattaaagg tgcatatcgt atacacctaa tgcgactgac caccttctcc
    17041 gactacagcc tgcgcgtgct gatgttcgtc gcggccgcgc cggaaggccg cgccaccatc
    17101 tcgcaggtgg cgagcgcgtt caacatctcg gagcaccacc tcgtcaaggt ggtccacttc
    17161 ctcggcaggc acggcatcct tcttaatacc cgcgggcgcc gcggcggctt gcagctcgcc
    17221 cgcccggcgc acgccatcaa cgtcggcgag ctcgtgcggc tgaccgaagg cggcgacatg
    17281 cctgccgaat gcttcgacaa gcgcaccaat acctgcgcgc tcgcgggcgg ctgccggcta
    17341 cagggcatgc tcaagcgggc ggtggcgagc ttctacgaag cgctcgcccc gtactccgtc
    17401 gcagacctgc aagcgccgcg ccgcctcgcg aggatccttc cctttcatag tgcgagccaa
    17461 tgcacggcat cggcacaatc aatttcccat cgcgtcatta acggcgtgaa atgacctgac
    17521 gggagcaccc caaggagaga acaatggaca tgaaggtcaa ggacaagagc ccgggccagt
    17581 gcccgttcac ccacgtcgcc ggcgtcggcc gcaccaaccg cgactggtgg ccgaagcagc
    17641 tgcggctgga gctgctgcac cagcattcct cgaagtccga cccgatgggc gggggctttg
    17701 actacgcaag agaattcaag agcctcgact acaaggcgct gaagaaggac ctggtcgcgc
    17761 tgatgaccga ctctcaggac tggtggccgg cggacttcgg ccactacggc ccgttcttca
    17821 tccgcatgtc ctggcacgcc gccgggacct accgcgtcac cgacggccgc ggcggcgccg
    17881 gccgcggcca gcagcgcttc gcgcccctcg acagctggcc cgacaacgtc aacctggaca
    17941 aggcgcggcg cctgctctgg ccggtcaagc agaagtacgg caagaagatt tcctgggccg
    18001 acctgctgat cctcgccggc aacgtggcgc tcgagtcgat gggcttcagg accttcggct
    18061 tcggggccgg ccgtgccgac gtctgggagc cggacgagga cgtgaactgg ggcgaggaga
    18121 tcgcctggct gggcgtcgac aagcgcttca gcggcgatcg cgacctgggg ccgttcggcg
    18181 cgacgcacat gggcctcatc tacgtcaacc cggaaggccc gaacgcgagc ggcgactaca
    18241 tggccgccgc caaggacatc cgtgccacct tcagccgcat ggcgatgaac gacgaggaaa
    18301 ccgtcgccct gatcgccggc ggccactcct tcggcaagtg ccacggcgcc gctcccgaat
    18361 cgcataaggg cccggatccg gaaggcgcgc cgctggaagc gcagggcctg ggctggacga
    18421 gcaactacgg ctcggggcac ggcgccgaca cggtttcgag cggcctcgaa gtgacctgga
    18481 ccaagacccc ggcgctgtgg agcaacagct tcctcgagct cctgtacaag tacgaatggg
    18541 agctgaccaa gtcgcccgcc ggcgccaagc aatgggtggc gaagaacgcg ccggaaatca
    18601 ttcccgacgc gcacatcccg ggcaagttcc acaagccgac catgctgacc accgattgca
    18661 cgctgcgctt cgatccggcg ttcggcaaga tctcgcgccg cttcctcgac gatccgcagg
    18721 ccttcgccga tgccttcgcg cgcgcctggt tcaagctgac ccaccgcgac atgggcccga
    18781 tcgcccgcta cctcgggccc gaggtgccga gcgaagagct gatctggcag gacccgatcc
    18841 ccaaggtcga ccacaagctg atcggcgaga aggagatcgc cgagctcaag aagaagatcc
    18901 tcgcctcgaa gctgtcggtg gcgcagctcg tgtccaccgc ctgggcgtcg gcctccacgt
    18961 tccgcggctc cgacaagcgc ggcggcgcca acggcgcgcg catccggctc gcgccgcaga
    19021 aggactgggc cgtcaaccag ccgctcgcaa aagtgctgaa gacgctcgag ggcatccaga
    19081 aggggttcac gaagaaggtc tcgcttgccg atctgatcgt tctcggcggc tgcgccgcga
    19141 tcgagcaggc cgcagccaag gccggcgtcg ccgtgaaggt gccgttcaag cccgggcgca
    19201 tggacgcgag ccaggcgcag acggacgtcg agtccttcgc ggtgttcgag ccgatcgccg
    19261 acggcttccg caactacctg aaaggccagt acccggtctc cgccgagcag ctgctcctcg
    19321 acaaggcgca gctcctgacg ctcacggcgc ctgagatgac ggtcctcgtc ggcggcctgc
    19381 gcatgctggg gaccaacgcc ggcggcgtga agcacggggt cttcacctcg aagccggaga
    19441 cgctgagcaa cgacttcttc gccaacctgc tcgacatgga caccgagtgg aagccgacct
    19501 cggaggcgag ggagctgttc gaagggcgcg accgcaagac cgggaaggtc aagtggaccg
    19561 cgacccgcgt cgacctcgtg ttcggctcga actcccagct gcgagccctc gccgaggtct
    19621 atgcgagcgc ggacgcgaag gagaaattcg tgaaggactt cgtggcggcg tggaccaagg
    19681 tcatgaacct ggaccgcttc gagctcaagc gcggctgaga atccgctgca cgaaggaggc
    19741 tacctgcgtg tttccgcggt cagcgccttc ggcggtggat ctctacgccc ggaagctcaa
    19801 cgaggcgctc gcgcaggccg tcgcggcggc ccggaagcga gtgaacgatc aggctggcga
    19861 tctccaccgg cggcccaccc aggacttgtc ttctggtcag caagctgcct cctctttcgt
    19921 cagctcagta ccgcggatcg tacatctgct tctggacgtg ggcgagcagg tccctcggag
    19981 cctcgcctag cgcgaggccg cgctcgtacg cgacccgcgc gaccgccgcg gcgatctgcg
    20041 cggaaacttc gcggatgcgc ggcagcgcgg gatagaggct tccctgcgcc aggtccgcct
    20101 cggtcaccag agcggccagc gtgcgcgccg cggccatgaa catctcgtcg gtcacgcgcc
    20161 gcgcgccgct cgcgatcacg ccgagcccga cgcccgggaa gatgtaggag ttgttgccct
    20221 ggcgcggcac gaaggtccgg cccccgagcg tcaccgggtc gaacgggctg ccgcaggcga
    20281 acagcgcgcg gccctgcgtg tggcgatagg cctgctcggc ggtgcattcc gccttcgaag
    20341 tcgggttcga cagcgcgaac acgatcggcc gctggttcag gcgcgccatg gtctcgagca
    20401 cctcgggcgt gaacaggccc ccggtggcgg cgacgccgat gatgccggtc ggcttgagcg
    20461 cgcggatcgc cgcgtcgaac tgcgcgaccg gtgcgtgagc gtgcgcgtag ggccgcttgt
    20521 gctcggcgag ctcgccgcgc cccttgacca ccaggccctg cgagtcgaaa agccagttgc
    20581 gcgagcgcgc atcggcctcg gaggtcccct cggccatcat tgccgacacc accaagtcgg
    20641 cgatgccggt cgccgcctcg cccgcgccga gatagagcag cacctgggcg gacagcctgc
    20701 cgccggaggc gcgcagggcg caaagcacgc cggcgagcgc caccgccgca gtgccctgga
    20761 tgtcgtcgtt gaaggtgcag atccgctcgc gatacttctt cagcaggcgg aaggcgttgc
    20821 gattggcgaa atcctcgaac tggatcagca cgccgggaaa cacctgctgc gccgcggcga
    20881 cgagttcctc caccagcgcg tcgtattctg ggccgcgcac gcgcttgcgc cgcaaaccca
    20941 cgtagtacgg atcggcggcg agtgcttcgt tgtcggtgcc tacgtcgagc atcaccggca
    21001 ggcaaagctc cgggtgcacc ccggcacacg ccgcgtagag cgagagcttg ccgaccggga
    21061 tgcccatgcc gttggcgccg aggtcgccca gcccgaggat gcgctcgccg tcggtcacca
    21121 cgatgagctt cgccggatag ggccagttgc gcaggatctc ggcgacgcgg ccgcgatcgt
    21181 tggcgccgat gaacagcccg cgcgggcgct ggaagatatg gccgaacttc tggcaggcga
    21241 ggcccaccgt cggcgtatag atgagcggct ggatctcgtc gatgttgtcg cacaccacgc
    21301 ggaagaacag cgcctcgttg cggtcgtgca gcgcgttcag cgcgacgtac ttgtcgaggt
    21361 cgctcggcag gccgcgcagg ttcgcgagca cgcggtcggc ctgctccttc agcgacagga
    21421 tgtgggccgg cagcaggccg cgcaagccga gctggtcgcg ctcgcgctcg gtaaacgccg
    21481 tgcccttgtt gagaagcgga tcgcgcaaga gcgcgatgcc gcgcgccgcg ccggatgcgg
    21541 ccatgcccgg aagactgctc atcggaacct ccctcggtgc tttgccggac aagtgtcgac
    21601 cgcgtggggc atcgcctgtt gacctaggtc aaagcgtctt caccacgccg gctcaacatc
    21661 agagccactt cgtcacaatg gctggaggag gcgtatgaga acgctattgg tggctgcctg
    21721 cgcggcgctg atcccggcgg gcccggcgca cgcgcagacc gcagagcgca ccggcgagca
    21781 ggtcgtcaag gcgcagtgcg cgaaatgcca cgagcagggg ctgcgcggcg cgccgaagat
    21841 cggcgaccgg caggcgtggg cgcctcgcat gaagctcgga cttgacgcga cggtgcgctc
    21901 ggcgatcaag gggcacggcg gcatgccggc gcgcggcggg atggccgatc tcaccgatac
    21961 cgagctgcgc gccgcgatcc tgcatctctt caaccccgcg gcagcggctt cggccaagct
    22021 cgaggccgcg ccggctgccg ctccggacca caacaagaag gtggtaggcg gcgtcgagat
    22081 ctatctcggt ctcatgccgg ccggcccgaa ggggctctat cacgtgaaca tctcgctgcg
    22141 ggacggcgca agcaaggccc tgatcaagga cgccgttgtg gaagcccgcg tggcgagcac
    22201 gctcggcggc acgacgaaga agctcaatgc gaccaccatc aacgaaagcg tgagctacag
    22261 caacgacttc aggatggtgg ccgacgagcg ctacaccctg accgtcactg tgcggctgcc
    22321 gaaggctgcg gcgccgcttg aagccaagtt cgacctcaag cggtaaccgg tatttgatcc
    22381 aagtcagaac agccccatct cccacggagc ctgccatgag aatgctcgag ctgaccgctg
    22441 ttgcatccgc aatcgcactg atctcggggg tcgtgcatgc cgccgatcca gccaagatca
    22501 actggagcaa ggtaccgacc aagaccgtaa cgcttttcta cccagggcag acctcgtacg
    22561 agtgggtgcg cggcagcgct catcccggcg cgcaagcggt ccagggtggc gaggcctgcg
    22621 ccacgtgcca caccggacaa gagaaggcaa aaggcgacaa actggtgaag ggtggaccgc
    22681 tcgagcccgc gccggtcaaa ggcaagaacg gctgggtgga cctcaaggtt caggccgcct
    22741 atgacgccag caacgcctac ttccgcttcc agtggaagac taagaatggg tatcccggaa
    22801 ccgagcacca gtaccttcgc ttcgacggca cggaatggaa ggtttacggc tatcccaagc
    22861 tcgacaaggt ggtccaggag ggcaagcagc cgggcatcta cgaagatcgc atgaccctca
    22921 tgatcgacga cggcaaggtg gcgggattcg cgaagcaggg ctgctggctc acctgccacg
    22981 atggccagcg cgacatgcag aaagtcgtat cgaaggaaga cgcgaacgcc aacgcgctgc
    23041 tcagcgccat caagaagaat gacctgcgca agtacctgcc tgccacgcgc gccgacccgg
    23101 cggactggaa gaccggcaag tcgctcgagg agatcgccaa gatcaagacc gaaggcggct
    23161 tcgtcgacct gatccagtgg cgtgggcacc gctcgaacgc ggtgggaatg gccgatgacg
    23221 gctacgttct cgagtaccgc ctctcggacg ccgggaagga catgttcagc ggcaacgccg
    23281 atccgaagac gcaccagccc aagttaatgt gggacccgaa aaaggtcggt tacaaatcca
    23341 tcaccgagaa gcagctgcgc aagggccagc atttcctgat ccgcgaaaag aacgcggtgc
    23401 cgttcgaccc gaacgcgggc tggaaagagg gtgacatgat tccggactac gtcgtgagcc
    23461 gcgaggacgc caaaggctcc gccgctgaca acaacgcgac cgcgagctgg aagaccggca
    23521 cgtggaccgt cgtcatcacc cgtccgctca accttgccaa cgaggatgac aaggcgctga
    23581 aggaaggcgg tgtctacaac gtcggcttcg cggtgcacga cgacaacatc accacccgcg
    23641 gacaccaggt gtcgttcccg gtgacggtcg gcttcggcgc gaaggctgcg atcgaagcga
    23701 ccaagatcaa gtaaggcaaa gcggctcgat gagaccggga ggacttctgc tcgcggtcgc
    23761 gggcttgctt ttcgcaggcg cggcgagcgc gcaatcgggc gaggcgctgc tcgaggcaaa
    23821 gggatgcctg ggctgccatg gcatcgagga aaagcagatg ggccccgcgc tgcgcgaggc
    23881 ggcggcgaag cacaaggggg cgccggccaa gctgatcgcc gcgctcaggg agggcaaggg
    23941 ccatccggtg aaggtcgagg ccacggagcc ggagctgaaa gcgatgatcg gcttcctcct
    24001 ggcggcgcaa ccctcgggcg ccaagccggc cgcggcggcg gcaggatccg gcaacgcgac
    24061 ctgtctcgcc tgccacggca acgagaaatt caccccgcac ctggaacagg agaggttcga
    24121 gaagagcgtg cacggcaagc gccagtgcgt cgagtgccac aaggacatca cggagatccc
    24181 gcacaaaaag gtcgcgcaca aggtgagctg cgtcacgtgc cacgacgacc tgtggaaggc
    24241 cgcgcagaag gaaggcaaga cccaggagca cgcgcggctc ggcgtggtgg tcgagcagat
    24301 cgaccgctac atgagatcca tccatgcgcg gcccaaccgc gaagaccagt cgcgcaccaa
    24361 cgcgacctgc tacaactgcc acgaggcgca ttacgtctac ccgctcggca gcgagcagcg
    24421 cgcagaatgg tgcctgagca ttccgaaggc ctgcggcgcg tgccatgcca agcagctcga
    24481 ggcctacgcc acttcggtgc atggcaggga aacgctgcag aaaggcaacc cggcgacagc
    24541 ggtgtgctcc gattgccaca ccacgcacga catcgatcgc ccgaccaagg aatcgatgaa
    24601 gctcgccatc acgaagaact gcggcacctg ccatgcggcg agctacgaga gctaccgcga
    24661 cacgtaccac gggcaggtcc acacgctcgg ctatgcctac actgccaagt gctacgactg
    24721 ccacggcagc cataccgtgc agcgcgtgtc ggacccgtcc tcgtccgtgc accccgacaa
    24781 tcggctcaag acctgccagc gctgccacgc tggcgcgacc gccgggttcg tgagcttcca
    24841 gccgcatgcg accacggacg acttccatcg ctttccgcaa ctctggctgt cggccaagtt
    24901 catgatcggg ctgctggccg gcaccttcgc cttcttctgg acccataccg cgctctggtt
    24961 cttccgggag tacagggagc gccagagccg cacgccgcgt ccgcacgtga tgacgcacga
    25021 aatgccgcag gcgcagcaaa agcattaccg gcgcttcacc gcgatatggc ggctcgcgca
    25081 cctgctcttc gccgtgagcc tgatggtgct caccctgacc ggcatggcgg tgctgtacgc
    25141 cgacagcgcg tgggcgcccg tggtgatcaa cgcgctcggc gggccgcaga gcgccgccct
    25201 catccatcgc gcgtgcgcgg tgatcttcgc agccgtgttc ctggcgcacc tcgtctacat
    25261 cctgaaccgc gtccggaagt ggcgcacgct cgcccaatgg ttcgggccgc attccctgat
    25321 ccccaactgg caggacctca aggacatcta cgcgatgttc aagtggttcg tcggcagggg
    25381 gccgcgcccg gtgttcgacc gctggaccta ctgggagaag ttcgactact gggcgccgtt
    25441 ctggggcgtc accatcatcg gcgtgagcgg gctcatgatg tggttcccgg cggcgaccgc
    25501 ctcggtgctg ccgggctggg tgttcaacgt ggcgacgctg gtgcacggcg aggaagcgct
    25561 gctcgcggcg ctgttcctct tcacggtgca cttcttcaac aatcacttcc ggcccgacaa
    25621 gttcccgctc gacgtggtga tgttcaccgg cacggtgccg gtggaggaat tcaggcgcga
    25681 gcacacgctc gagtacgacc ggctggtgca aaccggcgag ctgcagaagt acctggtcga
    25741 cgcgccctcg cagccgatga cgctcggctc gaagatcctc ggcttcaccc tgatcgcctt
    25801 cggcctcgcc ctgctccttc tggtgctgat cgggttctcc ggcgccaacg gcccctgacc
    25861 atgcgcctcg cccctgccct gctgctgatc gccttcgcaa tgacgcccgg gtccgccgcg
    25921 gcgcagccgg cgcggaccaa gcccgccgcc ggcgacgatc tgcgcgcgct ctacgtcaac
    25981 gcccccgaca tcgccgaggg caagcgcgtc gcggagtcgt cgtgcgtacg ctgccacggc
    26041 gccaacggca tcagccccgc gaaaggcacg ccgcacatcg ccggccagcg cgcggcctac
    26101 ctctacgcgc aactgcgcgc ctaccagcag cgcccgccca cccagagccc gatggcgggg
    26161 gcgattcgct tcctcaagga cgacgcgctc gtcaaggtgg cggcgtatta cgcgggcctg
    26221 gagccgccaa ggccgagcgg cggcaaggcg gcagcggtcg aggacccgct gcaggccggc
    26281 aaggcggcgg cggcggcctg cagctcctgc cacggcgaga gcggcgtcac cgcgatcccc
    26341 gggatgccca gcctcgccgg gctcgatccc aagtcgttcg tcgccgccat ggacgcctac
    26401 aagcgcggcg aacggccgca cgacatgatg aagtccttcg ccgcgcccct gagcgaggcg
    26461 gcgctgaaca acctcgcgct ctactacgcc ctgctcaagc ccgagcgggc caggacgccc
    26521 gccgccgggg atgccgcggc gggcaagaag gctgccgccg cctgcggcgg gtgccacggc
    26581 gagaccgggg tgagctcgat ccccggcacg cccagcctcg cgggccagga cgcgcagtat
    26641 ctcgcggcgg cgacgaacgc ctacaaggac ggaacgcgca aggacgagac catgaagggc
    26701 ccggccgccg cgctcgacgc gcgcgcggtg caggacgtgg ccgcgttcta cgccgcgcag
    26761 cagccgcagc cgccgaacgt gcgcaagccc ctcagcctcg ccgagtggat cgagcgctgc
    26821 gaccgctgcc acggcccgaa cggcaacagc atcgagccgc tgatgccggc actcgccgcg
    26881 cagcgcgccg actggctgga gatcgtgctc gcggcctacc gcagcggcgc gcgcaagagc
    26941 tcggccatgg gcgcgatgtc ggcctcgctc tccgaatccg atatccgtgc gctcgccgcg
    27001 cactactcgc ggcaggcggc gcgcccggtg gtctacgtgc tggttccgga gaaacggcca
    27061 tgaacgatac gccgctgcac cgcgagttcc gccacttttc cgagatgccg ctgagcctgc
    27121 gcgtgctcta caccgcgacg ctcctcgtcc tcggcatggg ctacctgttc gcgctgatct
    27181 atctgttcca cacctactcc ggcaaggacg gcaacccgct cgacctctcc tatgacgaca
    27241 tcgtcatcgc ctacaccggc agcggcaaga gctcgcgcct ggagtctgcg ctgcgcggct
    27301 cgatgtcggt catgctgccc aaggaggagc tcgcgccgct ggtggcctgg gtgcacgaag
    27361 gcgccgagcg cgccgcgttc gagaagcaga tccggcccac gctggagaag cgctgcctcg
    27421 cctgccacga cggcagcaac ccacacctcg ccaacctgaa cggcttcgac aacgtgaaga
    27481 aagtgaccga gcgcgatacc gggaccggca tcttcacgct ggtgcgcgtg tcgcacatcc
    27541 atctcttcgg cctcacgttc gtgttcttca tcgtcggatc gatcttcagc cacgcctacg
    27601 tgcggccggt gtggctgaaa tgcgcggtca tgatcctgcc tttcttcgcg ctcacgctgg
    27661 acgtcagctc ctggtacttc accaagctgt accagccgtt cgccttggtg gtgatggcgg
    27721 gcggcgggct gatgggcgca tcgttcgcct tcatgtgggc ggtctcgatg taccagatgt
    27781 ggttctcgcg cacgcccgcg gccgtcatgc acaggagcca gcaatgaaac gtcttgcgct
    27841 aggcctcgcc gtcctggcgg tgctcgcagg ctgcgacggc aaaggccccg cggcgaccca
    27901 gccgcaggcg ggcaatgtcg aagcgggcaa ggcgttcgcc gagcgcgaat gcaagggctg
    27961 ccacggcctc gacggcaagg gcatcgctcc cgccatcccg cacctcgctg cgcagcgcag
    28021 ccgctacctg ctcgcggcgc tgacggctta ccgggaaggc cggcgcacgc acgccgccct
    28081 gaaggagatc gcggcgcaca tgaccgaagc cgacgcgcgc aacctcgcgg cgtattacgc
    28141 ggccctgcct ccggtgcccg ccggcgcgtc gcaggccgcc cagcgcctgt ctccctacga
    28201 gcagggcaag gcgctcgccg cggcgtgcgc caagtgccac ggcgacgacg gcaacagcaa
    28261 gaccccgggc attcccggcc tggcaggcca gcagccgcac tatctcgtga tcgcggtgca
    28321 ggagtacctg aacaaggagc gcaaggcggc gcccatgcac gcgttgctcc cgggactgcg
    28381 gaagcgcgac gtcgagaatc tcgccctcta cttcgcctcg cagacgcccg cgccgcgcgg
    28441 ggcacctccc gtcggcgacc cggcggccgg cgagccgctc agcgcggtct gcggcggctg
    28501 ccacggctcc cacggcgtga gcctcgacgc ttcgacgccg atcctggccg gccaggatgc
    28561 gcgctatctg gccggcgcga tcaaggccta tcgcacaagc cgcgagcgcg aaatgatgcg
    28621 ccgctacgtc gtcggcctga gcgagaagga catcctgaac atcgccgcgt tctactcggt
    28681 ccagaaatcg cgcccggcgg agaaaggcca gaccctggtc caggacctgc gcgacaagtg
    28741 cgagcgctgc catggcccgc aggccgccga caacccggag atggcgatcc cgaagatcgc
    28801 cgggcaggac cgcgactacc tggtgatggc gctgcgcgcc taccgcgacg accggcgcga
    28861 aagctcgatg atgcacaaga tgagcctgcc ctacagcgac tcgatcatcg agagcctggc
    28921 ctcggtctac gccagccagc cggccaaata gctacttggt agcgaggacc gccttcacca
    28981 gcgcctggat ctcggcgtcg ctgccggcga tcttcggatg acccttgcct tccttgagct
    29041 tcgccgtcag cgcggcctcg gcgcccttgt cgcccttgta cttcgcagcg atgtccttga
    29101 gcgccggccc gaccttcttc ttgtccgact cgtggcagtt caggcagccc ttcgccttca
    29161 ggtcctgcgc ctgagccagc ccggccgcca gaaagccggt aacagccaga gctactagtg
    29221 cgactctcat cggttccttt cctttcaagg tcgtcgacgg cctagaactt gtatttgacc
    29281 acgccgtaga agatgttcgc ttcgtagttc tggaatgaaa actgcccggt gacgatgctc
    29341 gactgggtgc cggtcccgac gacgtaccgg gtgccgtcgt aggcgatgtc gctgtagcga
    29401 tatttctcgt aggcatagcc ggcggtgaat tcccagtgcc ggtcaagcgc gtacacccct
    29461 ctcaggttga acgcgacgcg ggtcgtgtcg tcgctcgcga cgatcgacgg ccgcggcgtg
    29521 ggatcgccgc ccggctgcac gctgaagtcg gtgtgcccat cggtctcggc gtagatcagc
    29581 gaagccttca gcgtcaggcg cgtgagcacg gcccagtcgg cgccgatgcc gatctgccag
    29641 gagttgtcct cgttcttcga gccccagttg taggtcccgg cgttgggcgg cgtcgccgga
    29701 tcggggttgc ccgttcccac gcggtgggcg gaatcgaact tgacgtactc gatgtcgccg
    29761 aagaggtgca cgcgccagct cttcgggtcg ccgtagccga cgctcgcgta gacctcgtga
    29821 cgcgtgtcct cggtgcggcc gagcggcgtg tccttgtatt ggttctcctt gtagatcgcc
    29881 tccaggccga ggtcgaggag cggcgcgggt gcgaagtcga gcgcgagctt gaccaggctc
    29941 tggtcgacgt tggcgacgtc gaagcgccgt acgaagagat ccatcgggtt ggcggcgagg
    30001 agggtcgggt gcgggttgaa atccccgcgc cgctgcaaca actggtactt gaggcgcgcc
    30061 gtcagccagt cgagcgagct gttcttccac tcggcgaaca gccgcgtctc ctcgttctcc
    30121 tggaaatccg cgcgctcgcg ctcgatgtcg gtccattcca ggccgccggt gagcttgttc
    30181 gcccgcgtca cgcgccagcc cgcctcggcg ccgtactggc gcttctcgta ttcgaagagc
    30241 tcgggagtgc agtcggcggc ggtgcagctg gcgccggtca ggccggtgcc cgggttgacg
    30301 aaggagatgt gcgtcgagtc gttctcctcc ttgatccagt tgaagtagag cttggtgtcc
    30361 accgtctgcg tcgggcgcga gctgagcgac agcccgaacg tggtcttcac gatgtcgcct
    30421 ttgaagctcg ggtcgctcgg attggtggcc gcgttgaccc ccccggtcga cagcatcgtg
    30481 ggctgcaacg agacatcgtt ggtcagcttg ctgtaggtaa agcgccccgc gagcgtggaa
    30541 tcgagcgcga gttgccgcag gttgccgttg acgctgaggc gcgtgagctc gttgtcgggc
    30601 gccagcaccg tggtatcgag cccgttcgcg aaaaaccggt tcgaccagcg cagcagctcg
    30661 ttctcgttct cgaacgtgct gtggctcagg ttcaccgcga agtgcgcgcg gcgcgtggag
    30721 tagccgactt cggccgagta attgcgcgtc agccagtcga tcggcgatgg caggtcggtg
    30781 aagccgttgc ccgggctggt gccgttcgcg ccgccataga cgttgatgcc gtcgcgccgt
    30841 acttcgttgg cctcgacgcg gaagtaccag ggcgaggtct tttgcagctc gaacatgccg
    30901 ccggtgtcgc ggcgcttgta cgaatggtcg aacgagcccc agagcgggac ggccggtagc
    30961 ggcgaaggcg ccgtgagggt cgcgccgccg atccccgaga agggcgaaag cgcccccggg
    31021 ccggagccga agttgtgctg cagctgatcg ctgtagagcc ggtacttgaa ggtgttgtag
    31081 cgcccgccgt tcaggtcgag gtactggtcg tcgcggccga ggttctcacc gtaggcgttg
    31141 aagtagttct ggtcgctgcg cccgcgcagc tcgaagccga agatgcccga attgccctcc
    31201 tgcaggtcgc ggtactcgtt cagcttcgag ggatcctgcg cgtcgctgtc gacgttgcgt
    31261 acgcccgcgc tcaccgagcc cgaccactcc atcgcctcct gcgcgaacgc cggcgccgcg
    31321 atgaaggcgt ttgccacgac aaccgagagg agtgtgcgtt tcatgtgcgc cccctatcgc
    31381 aggaagaact tgccccgcat ggcgggagca ttgctgccgt ggatgtggta gtggcagttg
    31441 acgcagccgc gcgcgatgag ccgcgtgttg ggtgtagcgg cgttccatcc gccgctcccg
    31501 tcgtagatcg tgccggggtg acgcgaggcg tcatggcagt cctggcaaac gttgggcgcc
    31561 ttctcggcga gcaggcgcgc gtggttcgag ccgtgcgagt tgtggcaggt gaggcagttc
    31621 tcctccaccg gcggatggcc ccacacgaaa ggcccgcgct tctcggcgtg gcaggtcgtg
    31681 cacagctgcg ggatcgactc cgccttgacc attgctttgg agagcgcccc gtgcgggtta
    31741 tggcagttgc tgcaggtgac cttgccttcg atgatcggat ggtgcgaggg cttggtgagc
    31801 tgcgagcgga tctgccggtg gcacgaggtg caggtctcgt actcgagctg gcgctcggtg
    31861 gtcacgaacg ggctgatcgt cgagttgggc ttcttcagtg cgatggtcga gcccggcccg
    31921 ggcgtgccgt gcaggctgtg gcaattgctg cacgcgacgt cctgcttgcg gtgcgcgccc
    31981 gaatcccaga acgcgaggtg gcggtcgccc tggtggcaac tcaggcaaac cgcagccttg
    32041 cgctcggccg gcagggcttt gctgttgaag ccgagaatcc cgccgactcc cttgccgccg
    32101 cctgcggcca cgtgcgcgtc ggcatttccg tggcaggtct ggcagtccgg acctttcagg
    32161 ttgccgccct ggccgtgctt cgtcttcagg tagctctcga cgtagtcggc gtggcagttc
    32221 tggcagacgg cggcttcggg cgcgggtttt cgctgcgctg gcgcctgcgc cgatgcgggc
    32281 gcgattccca agagcaagcc ggcggcgaca gccgtcaaca gccgcaatgc gatgaagcct
    32341 gtgcgagacg ccatgccgct ctcccttcga ccggaccgcc ccactgcctc cggcgagcat
    32401 gccatcaggc atctcgaatc cgcgtttcgt atttcgaggc agcttgaccc aggtcaaaaa
    32461 ccgagccccc ggcgtgccgc aactgcagcg cgggaagtgt tgacctaggt caaagagcgg
    32521 ggatggcgtt gggcaacact gcggctagcc agcggtatga caatcccggt cgcagatgtc
    32581 gcacgcgaag cgcgccttgt acgaggcgtg atcgccaacc tcgccctgtt cagcggcgtc
    32641 gctccgggcc acgtctcgac ggtcgccagg cagtcctggg cgttgccggt gccgcgcggg
    32701 gcggtcatcg cccgccgagg cagccggctc gagggcgcct tcgcccttgc ctacgggttt
    32761 gtcgagctcg cgctgcgcga cgcgcacggc gtagagcgcg ggcttcgctt cgtcgccccc
    32821 ggccagacct tcggcgaggc gacggcgctg ctcgggcgcg cgtgcaaata cgaggcgatc
    32881 gcgctcgccg agtcgaagct gatcgtcatc cccgccgcgg cgctgttcgc gctgatggac
    32941 cacgatgcgc gcttcgcgcg caccctgctg atgacgctcg cgcagcgcga gctcgatctg
    33001 gtggccgaga tcgagtccgc cacgctgcac ccggccgagc agcggctcgc cgggtatctg
    33061 cattcgctcg ccgatccggg cgattgcccc gcggcctgca cggtgcgcct gccggtgagc
    33121 aagaccgcga tcgcttcgcg cctcgacatg aagaaggaaa cgctgtcgcg gctgctgcgc
    33181 gcgctcgtgc agcgcggcct gatcgcggtg aagcagcgcg agatcacgct gctcgatcgc
    33241 gcgcgcctga agcagatctg ctgatgatgc cgagctgcag cgcgagagcg cgcgatttgg
    33301 cgctggtcac ccaagtgatc ggcaagctgg cgctttttac cggtttgccg ccggcgattc
    33361 tcgccgacgt cgcaaggcag tcgtgggcgt tcgcggctgc gcgcggcacc actttcgctg
    33421 cgcgcggcgc gcttctgccc ggcatcttcg cgctcgcctg cggctcggcc cggctgacgc
    33481 ttcgagctgc gcacaacggc gagcgcgtgc tgcggctggt gtcgagcggc cacaccttcg
    33541 gcgagtcggc cgcgctgctc gggcgtgcca gcagctatga cgcgatcgcg cttgccgatt
    33601 gcaccgtggt ggtggttccc gccgctcaag tgctgtcgct gctcgagcgc gatgcgcgtt
    33661 tcgcgcgcca gttggtcacc gcgctggccg aaaccaagtt cgggctgctg tcggaactcg
    33721 agacggccac gctgccgcgc ggcgcgcagc gcctcgcgca ctacctggcc tcgctcgccg
    33781 agccggccga gctgcccggg cagttcgtcg ccaggctgcc ggcaagcaag acgctcatcg
    33841 catcgcggct cgacatgaaa aaggaaaccc tgtcgcggct gctgcgctct ctcgcggcgc
    33901 agggcgtgat cctggtaaag gggcgggaaa tcagcatcct cgaccgcgag cgcctcgcga
    33961 gaatcgcgcc gctcaacggc gagccgcatc cccttttgac ctaggtcaag ccgtccgcta
    34021 ccggacggtg gattccggcg ccggccgagg cacaccatga cctatgaggg tcgggctctt
    34081 cattccgtgc tacgtcgacg ctttctttcc gaaagtgggc atcgcgacgc tcgagctcct
    34141 cgagcgcttc ggcgtcgacg tggactaccc gctcgaccag acctgctgcg gccagccgat
    34201 ggccaacgcc ggctgccagg acgacgcggc ggcgaccgag cggctgttcg tcaggaactt
    34261 cgacggctac gactacgtcg tgaccccctc cgggagctgc gcgcaccatg tgcgcgagca
    34321 cctgaccgcc atcggccaga cggccaaggt gaagaaggtg cgccgccgga cctatgagct
    34381 ggtcgagttc ctgcacgacg tgctgcgcgt gcgcgacttt ccgtgggcga aatttccgca
    34441 caaggtcggg ctgcacatcg gctgcgccac gctgcgcggc ttgggcgccg cgaaaatgtc
    34501 cgagatcgaa gggccaccgt tctcgaagcc tctcaccctg ctttcgcgcg tgaaggggat
    34561 cagcgtggtt gtgccctccc ggcccgatga atgctgcggc ttcggcggca cgttctcggt
    34621 gttcgaggag ccggtgtcgg cgcggatggg gtacgacaag gtcaacgacc acaagaatgc
    34681 gggcgcgaaa tacatcgtct cgcaggacat gtcgtgcctc atgcaccaga agggatgcgc
    34741 cgagcgcctc ggcctgggaa tccgcttcat tcatatcgcg caggtcctga acgggactgc
    34801 cgcatgaagc gcgtcgatca tgccgaggcg tcctcgcgct tcatcgcggc gaaggagcac
    34861 gtcgagttcc acgacaagcg gctctgggac ctgcgccaga agcgcgaccg cgaggctgcc
    34921 gcgatccccg aatgggaaca gctcagaacg ctcgcctcga cgatcaagga gcatacgctc
    34981 gcgcgcctgc ccgactatct cctgcagttc gagcgcaacg cgaccgccag cgggatgcgc
    35041 gtgcactggg cgcgcgacgc cgccgagcac aaccaggtcg tgcatgagct gctgagcgcg
    35101 cacggcgcgc gcatcctggt gaagagcaag tcgatgctca ccgaggaatg cgagatgcgt
    35161 ccgttcctcg aaacgcgcgg catcgaggtc gtcgagaccg acctgggcga gcgcatccag
    35221 cagctcgacg acgagccgcc gagccacatc gtggtgccgg cggtgcacaa gctcagaacc
    35281 gacgtggcgc gggtcttcgc gcgcaccttg ggcaccgacg ccggcaacag cgacgtgccc
    35341 tacctcgccg aaagccagcg ccgcgcgacg cgcccctact acctcgaggc gcgggccggc
    35401 atgaccgggg ccaacttcgc ggtcgcggaa accggcagct tcgtggtctg caccaacgag
    35461 ggcaatgcgg atctgagcgc gaacctgccg aagctgcaca tcgcctcgat cggcatcgag
    35521 aagatgattc cgcgcatcgc cgacctcggc gtgttcatcc gcctgctttc gcgcagcgcg
    35581 ctcggctcgc cgatcacgca ggtcacgtcg cacttccgtg ccccgcgccc ggggagcgag
    35641 atccacgtaa tcctggtcga caacggccgc tccgagcgcc tgggaatgaa ggatttctgg
    35701 tattcgctga agtgcatccg ctgcggcgcg tgcatgaaca cctgcccggt ctaccggcgc
    35761 agcggcggcc tgagctacgg cgccacctac tcggggccga tcggcgtgat catcgacccc
    35821 gcgttcaacc tgcgcaagta cagcgcgctg ccgttcgcct cgacgatgaa cggcagctgc
    35881 agcaacgtgt gcccggtgaa gatcaacatc cacgagcaga tctacaagtg gcggcagatc
    35941 atcaacgagc gcaaccagct tcccatcgtg aagaaggaag cgatgcgcat ggcaggcaag
    36001 gtgctctcca gccccaagct ctaccgcgcc gcagtcaagg cggcgaacgc gggcctcgag
    36061 tacctgccgc gcagcatgat ctacaacccg ttgaacgcct ggggacgcag ccgcgaagtg
    36121 cccaaggcgg cgagcgccag cttccgcgac tggtacgtca agcaccgggg cgcaggcaaa
    36181 tgagcgcgcg cgacgacatc ctggcgcgcc tcggccgcaa tcttcccctt tgcccgccgc
    36241 tgcccgaaat accgctgttc gattcttctt ccccagacct gaaagaaaaa ttccgcgaaa
    36301 acctcgcccg gatgggcggc gagctgatcg agctgtcgcc ctccaggatc ccggcctgga
    36361 ttcgcgggaa gttccccgat ggggccgtga tctgctccgc gaccccggag gtcgccggca
    36421 accggccgct cgagtcgatg cgcgatccgc gcgagctcga gagcgtggac ctgggcgtgg
    36481 tgcgtgcgcg cttcggcgtg gccgagaccg gctcggtctg gctgagcgaa gccgagttca
    36541 aggtcaacgc gctcggcttc ctcccccagc acctcctcgt cctgctcgac ctgcgcaagc
    36601 tcgtgccgaa cctgcaccat gcctaccggg atcccggctt cctcgaggcg cgctacgccg
    36661 tgctcatgac ggggccgtcg gccaccgccg acatcgaagg cgtcctggtg cgcggggcgc
    36721 agggcgtccg cagcctgacg gtggttggat gttgagccag atcaacatgc cggcacccgc
    36781 cacacggtac taaggagcat gttccagatt cgcatccacg gccgtggcgg ccagggggtc
    36841 gtgagcgccg cggagatgct ctccgtcgcc gcctttctcg agggcaagca cgctcaggcg
    36901 ttcccgagct tcggctcgga gcgcaccggc gccccggtgg tcgcgttctg ccgcatcgac
    36961 gaaagcgaga tccgcctgcg cgagccgatc atgcggcccg atgcgctcat catccaggac
    37021 ccgacgctgc tgcaccaggt ggacgtgttc gcgggcctgg cgccgggcgg cacggtcctc
    37081 atcaataccg cgcgcagctt cgccgacctg ggcctgcagg acttcatcga gaagttcaag
    37141 gtctgcgcgc tgccggccac cgagctcgcc ctgaagcacg tcggccggcc gctgccgaat
    37201 gcggcgctgc ttggaggatt ttcagcgctt tcaggcagac tcgccctggg gtccgtgaag
    37261 gcggcgatcc gcgagaagtt ttccggcaag gtcggcgagg gaaacgtggc tgcggcgggc
    37321 gctgcccacg aatacgtgct gaaggagctt catgctgaag cagcttgaag gctcccacgc
    37381 agtggccgaa gcggtggcgc tgtgccgccc ggaagtcatc tgcgcctacc cgatctcgcc
    37441 gcagacgcac atcgtcgagg ggctcggcga gatcgtgaag tccggcgccc tctcgccgtg
    37501 cgaattcatc aacgtggaat ccgagttcgc cgcaatgtcc gtggcgatcg gcgcctcggc
    37561 ggccggcgcg cgcgcctata ccgcgaccgc gtcgcagggg ctgctcttca tggccgaggc
    37621 gctgttcaac gcctcggggc tcggactgcc gatcgtgatg acggtcgcca accgcgccat
    37681 cggcgcgccg atcaacatct ggaacgacca taccgactcg atgtcg
//
LOCUS       DATBSZ010000157         4237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_571385, whole genome shotgun sequence.
ACCESSION   DATBSZ010000157 DATBSZ010000000
VERSION     DATBSZ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571385"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VIG70_10385"
     CDS             <1..1179
                     /locus_tag="VIG70_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY8251004.1"
                     /translation="TALGIAALGSFVAGTVATLVIAAIGAPLTKLALLFGPAEYFGLM
                     VFGLGLAIVLARGSVLKAVIMVVLGLLLATVGTDLETGQDRLTLGIAELSDGVDFAVL
                     AMGVFGIAEILRNLERPESRDVVVSKIGRLLPSLDDLRRSLGPIVRGTGIGSVLGILP
                     GNGAVLGPFASYALEKKLAKDPSRFGRGAIEGVAGPESANNAGAQTSFIPLLTLGIPP
                     NAVMALMVGAMTIHGLIPGPQVMLKHPTLFWGMIASMWIGNLFLLIINLPLIGLWVRL
                     LKVPYRLMFTTILLFCAIGIYSINNNPYDVYFTAFFGLAGYVFIKLGLEPAPMLLGFV
                     LGRLMEEKLRQALALSEGSFMTFVERPVAAGLLVLAAAVMVIAVLPAVSRSRAKAFQE
                     "
     gene            1197..2156
                     /locus_tag="VIG70_10390"
     CDS             1197..2156
                     /locus_tag="VIG70_10390"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY8251005.1"
                     /translation="MKRILASLVLSLFSGIAAAQAYPTRPVTIIVPFAAGGPTDIIAR
                     ITGEYFSKALGQQFVVENVAGAGGTTGITRGAQSKPDGYTIMMGHMGTHGAAPALYAN
                     LKYDPAKDFTPIGLVAGTPILIVAKKDFPAANLREFIAKVKDPAAKVKQAHAGVGSVS
                     FTTCTLLTSQLGAKSIVAAYRGTGPALNDLVGGQVDYMCDQIVNLVEQVKGGSIRAYA
                     IATTERSPALPDVPTTKEAGLPDYQVSAWNALFGPKGVPQDVAAKLSATLVKGLDDEA
                     TRKRLLDLGSVIPDRAGRSPQALQKLVESEVARWTPILKAAQQ"
     gene            2177..2710
                     /locus_tag="VIG70_10395"
     CDS             2177..2710
                     /locus_tag="VIG70_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8251006.1"
                     /translation="MAGWHADHVYFQRLLDLLRKELDVFHGGERPNYELMLDIITYLR
                     EYTDRFHHPREDAAFAFIMRRFPDMQLTLARLQQEHRVIAQAGDTLRQLLDAALEGEM
                     VARNEIEMAAATYLVYYGNHIAREEEDVMTRAALALSAEEWEAVRKNVPGGKDPVFGA
                     EPEERFRELRRRIALEA"
     gene            2720..3202
                     /locus_tag="VIG70_10400"
     CDS             2720..3202
                     /locus_tag="VIG70_10400"
                     /inference="COORDINATES: protein motif:HMM:NF042805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6781 family protein"
                     /protein_id="HEY8251007.1"
                     /translation="MENERETVRQSLAKTLRRTDEALKRSAEAGQETLRRLVETGREL
                     SGRELQQTFDNLQKIEEQLIATVGQAAASAAERMPEDMREASGRAARTSTESTRQAAA
                     AMTKLSQRLAGTSVELGLTCLQLSGEFGVRCAQIAGGWLASMAEAVDKPAADDASKKP
                     "
     gene            3212..>4237
                     /locus_tag="VIG70_10405"
     CDS             3212..>4237
                     /locus_tag="VIG70_10405"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEY8251008.1"
                     /translation="MLRHTLGMMRELPRLNEIASVFIRHGLGDFVQRIGIAGILERAG
                     QILRWGEAAESVRLEPPQRVRLALEELGPAFVKLGQVMATRVDLFPPSWIAEFEKLHA
                     AAAPVPFEALLPELERSLGRSPFEAFRDIETTAFAAASIAQVHRARLQDGTAVVLKVR
                     RPGVRAKVDADLRLLTRVAELLESEMPEARRYRPVEIAAQFRRSLEREMDFATETRNV
                     ERFAKNFAGDPHVIIPKVYPEYTSDALLVQERVEGIRASDPAAVEAAGLDRKTIAVRG
                     TQAFLKQILVHGFFHADPHPGNVIFLPDHRMVIIDFGMVGRLSPQRRKQVTDLLAGLA
                     HLEERPML"
BASE COUNT          682 a         1475 c         1424 g          656 t
ORIGIN      
        1 accgcgctcg gcatcgccgc gctcggctcg ttcgtcgccg gcacggtggc cacgctcgtc
       61 atcgccgcga tcggcgcgcc gctcaccaag ctcgcgctgc tcttcggccc ggcggagtat
      121 ttcggattga tggtgttcgg cctgggactc gcgatcgtgc ttgcgcgcgg ctcggtgctg
      181 aaggcggtga tcatggtcgt gctcgggctt ctgctcgcca cggtcggcac cgatctggag
      241 accggccagg accgcctcac gctcggcatc gcggagctct ccgacggcgt cgatttcgcg
      301 gtgcttgcca tgggcgtgtt cggcatcgcc gaaatcctgc gcaacctcga gcgtcccgaa
      361 tcgcgcgacg tcgtggtctc gaagatcggg cggctgctgc cttcgctcga cgatcttcgc
      421 cgctcgctcg gccccatcgt gcgcggcacc ggcatcggct cggtgctcgg catccttccg
      481 ggcaacggcg cggtgctcgg ccccttcgcg tcctacgcgc tggagaagaa gctcgccaag
      541 gacccgtcgc gcttcggcag gggcgcgatc gagggcgtgg cgggccccga gagcgccaac
      601 aacgccggcg cgcagacttc gttcatcccg ctcctcaccc tcggcattcc gcccaatgcg
      661 gtgatggcgc tcatggtcgg cgccatgacg atccatggcc tcataccggg cccgcaggtg
      721 atgctcaagc acccgactct cttctgggga atgatcgcct cgatgtggat cggcaacctt
      781 ttcctgctga tcatcaacct gccgctgatc gggctgtggg tgcggctgct caaggtgccc
      841 taccgcctca tgttcacgac catcctgctg ttctgcgcga tcggcatcta ctcgatcaac
      901 aacaacccct acgacgtgta cttcacggcg ttcttcgggc tggcgggata cgtcttcatc
      961 aagctcgggc tcgagccggc gccgatgctg ctcggcttcg tgctcggaag gctgatggag
     1021 gaaaagctgc gccaggcgct cgcgctctcg gagggaagct tcatgacctt cgtcgagcgt
     1081 ccggtcgccg ccgggcttct tgtgctcgcg gccgcagtca tggtgatcgc cgtgctgccc
     1141 gcggtgagca ggagccgcgc caaggccttc caggaatgaa ttcacaggag aaaaccatga
     1201 aacgcattct cgcgtcccta gtgttgagtc ttttttcggg tatcgctgct gcgcaggctt
     1261 atccgacgcg cccggtgacc atcatcgtgc cgtttgccgc gggcggcccc accgacatca
     1321 tcgcccgcat caccggcgag tacttttcca aggcgctcgg ccagcagttc gtggtggaga
     1381 acgtcgccgg cgcgggcggg acgaccggca tcacgcgcgg cgcgcaatcg aagcccgacg
     1441 gctacaccat catgatgggc cacatgggca cgcacggcgc cgccccggcg ctgtacgcca
     1501 acctcaagta cgacccggcg aaggacttca ccccgatcgg gctggtggcg ggaacgccga
     1561 tcctgatcgt ggcgaaaaag gactttccgg cggccaatct ccgggaattc atcgccaagg
     1621 tgaaggatcc ggcggcgaag gtgaagcagg cgcacgccgg cgtgggctcg gtttcgttca
     1681 ccacctgcac cctgctgacc tcgcagctcg gcgcgaagag catcgtcgcc gcgtaccgcg
     1741 gcaccggacc ggcgctgaac gatctcgtcg gcggccaggt cgactacatg tgcgaccaga
     1801 tcgtgaacct ggtcgagcag gtgaagggcg gcagcatccg ggcttacgcc atcgccacca
     1861 ccgagcgctc gcccgcgctg cccgacgtgc cgaccaccaa ggaagccggc ctgccggact
     1921 accaggtgag cgcctggaac gcgctgttcg ggccgaaggg cgtgccgcag gacgtcgccg
     1981 ccaagctcag cgccacgctg gtgaaagggc tcgacgacga ggcgacgcgc aagcgcctgc
     2041 tcgacctcgg cagcgtgatc cccgaccgcg ccgggcgctc gccgcaggcg ctgcagaagc
     2101 tggtggaaag cgaggtggcg cgctggacgc cgatcctcaa agccgcgcaa caatgaagcc
     2161 ggaggcgaaa agtccggtgg cggggtggca cgcggaccac gtctatttcc agcggctgct
     2221 cgacctgctg cgcaaggaac tggacgtgtt ccacggcggc gagcggccga actacgagtt
     2281 gatgctcgac atcattacct acctgcgcga atacaccgac cgcttccacc acccgcgcga
     2341 ggacgccgcc ttcgccttca tcatgcggcg ctttcccgac atgcagctca cgctcgcgcg
     2401 cctgcagcag gaacaccgcg tcattgcgca ggccggcgat acgctgcgcc agctcctgga
     2461 cgcggcgctc gagggcgaga tggtcgcgcg caacgagatc gagatggccg cggccaccta
     2521 cctcgtgtac tacggcaacc acatcgccag ggaggaggag gacgtgatga cgcgcgccgc
     2581 gctcgcgctt agcgccgagg agtgggaagc cgtgcgcaag aacgtgcccg gcgggaagga
     2641 tccggtcttc ggcgccgagc cggaggagcg cttccgcgag ctgcgccgcc gcatcgcact
     2701 tgaagcctag gagatcatca tggaaaacga acgcgagacg gtacgccaat ccctggccaa
     2761 gactttgcgg cgcactgacg aggcgctcaa gcgctcggcc gaagccgggc aggaaaccct
     2821 tcgccggctg gtcgagaccg gacgcgagct ctccggacgc gagctgcagc agacgttcga
     2881 caacctgcag aagatcgagg agcagctcat cgccaccgtc gggcaggctg ccgcgtcggc
     2941 cgccgagcgc atgcccgagg acatgagaga ggcgagcggc cgggcggcgc gcaccagcac
     3001 cgagagcacg cgacaggcgg cggcggcgat gaccaagctt tcgcagcgcc tcgccggcac
     3061 gtcggtcgag ctcggcctga cctgcctgca gctttccggc gagttcggcg tgcgctgcgc
     3121 gcagattgca gggggctggc tcgccagcat ggcagaggcc gtcgacaagc cggccgccga
     3181 cgacgcaagc aagaagccct aggccgcccg catgctgcgg cacaccctcg gcatgatgcg
     3241 cgagctgccg cgcctgaacg agatcgcctc ggtcttcatc cgccacggcc tgggcgattt
     3301 cgtgcagcgc atcggcatcg ccggcatcct cgagcgcgcc gggcagatcc tgcgctgggg
     3361 cgaggcggcc gagtcggtcc ggctcgagcc gccgcagcgc gtgcgcctcg cgctggagga
     3421 gctcggcccg gcgttcgtca agctcgggca ggtcatggcg acgcgcgtgg atctttttcc
     3481 gccgagctgg atcgccgagt tcgagaagct gcacgccgcg gccgcgccgg tgccgttcga
     3541 ggcgctgctg cccgagctcg agcgctcgct cgggcgctcg ccgttcgagg cgttccggga
     3601 catcgagacc acggcattcg cggccgcctc gatcgcccag gtgcaccgcg cacgattgca
     3661 ggacggcacc gcggtcgtgc tcaaggtgcg ccggcccggg gtacgtgcga aagtcgacgc
     3721 cgacctgcgt ctgctcacgc gcgtcgccga gctcctcgag tccgagatgc ccgaggcgcg
     3781 gcgctaccgc ccggtcgaga tcgcggcgca gttccggcgc tcgctcgagc gcgagatgga
     3841 cttcgccacc gagacgcgca acgtcgagcg cttcgcgaag aacttcgccg gcgacccgca
     3901 cgtcatcatc ccgaaggtct accccgagta cacgagcgac gcgctgctcg tgcaggagcg
     3961 cgtcgagggg attcgggcga gcgatccggc agcggtcgaa gccgcggggc tggaccgcaa
     4021 gaccatcgcg gtgcgcggga cgcaggcttt cctcaagcag atcctggtcc acggcttctt
     4081 ccacgccgac ccgcaccccg gcaacgtcat cttcctgccc gaccaccgca tggtgatcat
     4141 cgacttcggc atggtcgggc ggctctcgcc gcagcgccgc aagcaggtca ccgacctgct
     4201 cgccgggctc gcgcacctgg aagagcgccc gatgctc
//
LOCUS       DATBSZ010000158         5806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_25061, whole genome shotgun sequence.
ACCESSION   DATBSZ010000158 DATBSZ010000000
VERSION     DATBSZ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25061"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..752
                     /gene="pth"
                     /locus_tag="VIG70_10410"
     CDS             75..752
                     /gene="pth"
                     /locus_tag="VIG70_10410"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY8251009.1"
                     /translation="MIRLVVGLGNPGKEYERTRHNAGFWLVERFAQANGMVLRKDPKF
                     KALVGKTSNGAWFLLPQSFMNLSGQPVQMLAGFFRIKPEEVLVVHDELDFPPGTVRLK
                     QGGGIAGHNGLKDISQRLATHEYWRLRLGVGKPPVGTEGADYVLQKPSAEDRAAIDAA
                     IQKSLEVLPQILAGDVQAAMNKLHTEEPKKAEPAKKVEAPKKVEEPKKEIPAEKPGLL
                     KSLFGKK"
     gene            749..1831
                     /gene="ychF"
                     /locus_tag="VIG70_10415"
     CDS             749..1831
                     /gene="ychF"
                     /locus_tag="VIG70_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY8251010.1"
                     /translation="MKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIVPV
                     PDRRLDVIAEIEQPQKVVPAVVEFVDIAGLVAGASKGEGLGNKFLANIRETEAIVHVV
                     RCFEDANVVHVAGKVDPVSDIETINTELALADLDSVEKQIAKHAKLAQTTGDKETVRL
                     VSALTKVRDVLDAGRPARVAELSKEERAVVAPLFLLTMKPTLYVANIAEEGSGALLEK
                     VRAHAAKEGAPVVPVSAAIESQIADMSDEDKQLFLEDIGMDEPGLNRVIRAAYAILGL
                     QTYFTAGPKEVRAWTVRAGATAPQAAGVIHTDFERGFIRAEVASYDDYVACGGEQGAK
                     EAGKLRLEGKEYTVRDGDVIHFRFNV"
     gene            1883..2266
                     /gene="crcB"
                     /locus_tag="VIG70_10420"
     CDS             1883..2266
                     /gene="crcB"
                     /locus_tag="VIG70_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEY8251011.1"
                     /translation="MKSVLLVFAAGGLGCVARYKLGALVLHHTIAWKFPAGTFAVNVI
                     GCLVAGLLIGLAENHGFLTSEVRLLLFTGFLGGFTTFSAFGVETVALMQRGDFAVAAS
                     DVAASVVFGIVALWGAVKLAGLGSG"
     gene            2343..3428
                     /locus_tag="VIG70_10425"
     CDS             2343..3428
                     /locus_tag="VIG70_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8251012.1"
                     /translation="MNLRRLRLVTGLILMAFVIGHMANLMLGMHSLAAMESGRPWLMG
                     PWRSIAGQGLLVCSALIHLALGVYAIAARRSLAISKTDVVQLCLGLATPPLLLNHAVV
                     MHMAGEVSPNFDATFGQMLAVYWSFSPGYAFQQLGVVMVVWIHAAVGLYSWLVLKPVW
                     KRVGGLILPLLFAVPILALLGFAEAGREVLEKLATDAAWREHITANLRRVVAVTDPLN
                     AMQSRLLWGYGMLVLVALGVFGARVLRERLQRVTIGYDGGVSAQGRRGLSILELSRLN
                     DVPHAHVCSGRGRCGTCRVRIESGAGQLTPMTEAERGTLARVGAGAGERLACQVRVTG
                     SGVVAARVLPPYADASAARLPHEWVAP"
     gene            3425..5224
                     /locus_tag="VIG70_10430"
     CDS             3425..5224
                     /locus_tag="VIG70_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY8251013.1"
                     /translation="MSQTLAGWSVQALADAATRMETLRVDVLRAPEELRALAAATHAA
                     LMSASGVAAFTYVVILLLAGIGVEWLFWTYAYSPLRAIQSSVPESPRQALRLALRRLV
                     LVVSGLLLFTVAAIGASAAFSWPSGVQELVVAATLLVLVLRLAWVLICAVLAPGRPRL
                     RLVPVANERAGWLAAAAMTVVFLLALGSFVPNVMERIGGAGHAAGALRLLTTTAAAFL
                     LVVVAFAFFGARRGPRFPRSFVLALMVVVVYGIWLMNARAATVAAIVAVVIALQIGLR
                     ELVFFFWPDEDDRVAPSIVLSAARFVVVLLGLGAGALVIEAPVLALAAAESPMLRLVL
                     RLLGVATLALLTHVLWITVRTAVDQRLKRIRPAGPHYEPDEHSRLLTLLPLLRVTAAV
                     LLVVMLVISSLWALGIEITPVLAGAGVVGLAIGFGAQALVRDVIAGVFYLAEDAFRIG
                     EYIEAGGNTKGAVERITLRTVALRHHNGPLHFVPYGALGTVRNTSRDWVIEKFNLPLP
                     IDVDSEKVRKLIKKVGEEMLADPEIGRLIMEPLKGKLYRVDPGVKIFRCKFRTAPGKQ
                     FDVRAQAYKRIEAALRKMEVRFADGRLPVLTNA"
     gene            5221..5688
                     /locus_tag="VIG70_10435"
     CDS             5221..5688
                     /locus_tag="VIG70_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8251014.1"
                     /translation="MSETRFDVAKAADLPRLVELLRLLFESEAEFSADAEKQRAALQA
                     ILADPAKGRIFVARDGREVIAMASLLYTISTAEGGKAALFEDLVTAPEHRKRGIGAAL
                     LKHVIEQARADGVLRITLLTDMQNERAQAMYRRVGFVGSPMKPMRLKLKKGAK"
BASE COUNT          902 a         1904 c         2023 g          977 t
ORIGIN      
        1 gcgaagggcg ccgagaagcc cgccgcgaag ggcgccgaga agcctgccga caaggctgac
       61 aaaaaagaca agaagtgatc aggctagttg tcggcctggg caacccgggc aaggagtacg
      121 agcgcacgcg ccacaacgcg ggcttctggc tggtcgagcg cttcgcgcag gccaacggca
      181 tggtcctgcg caaggacccg aagttcaagg cgctggtcgg gaagacctcg aacggcgcct
      241 ggtttctcct gccgcagtcc ttcatgaacc tgagcggcca gccggtgcag atgctcgccg
      301 ggttcttcag gatcaagccc gaggaagtgc tggtggtgca cgacgagctc gactttccgc
      361 cgggcaccgt gcggctcaag cagggcggcg gcatcgccgg ccacaacggc ctcaaggaca
      421 tctcgcagcg cctcgccacg cacgagtact ggcggctgcg cctgggtgtc ggcaagcccc
      481 ccgtcggtac cgagggagcg gactacgtgc tgcagaagcc ctcggcggag gaccgggccg
      541 cgatcgatgc cgcgatccag aaatccctcg aggtgctgcc gcagatcctg gccggcgacg
      601 tgcaggccgc gatgaacaag ctgcacacgg aagagccgaa gaaagccgaa cccgcgaaga
      661 aagtcgaagc gcccaagaag gtcgaagaac ccaaaaaaga gatacccgca gagaagccag
      721 gcctgctgaa gtctctcttc ggcaagaaat gaaatgcggc atcgtgggct tgccgaacgt
      781 cggcaagtcc accctgttca acgcgctcac caaggccggg atcgcggccg agaactaccc
      841 cttctgcacc atcgagccga acgtcggcat cgtgccggtg cccgaccgcc gcctggacgt
      901 gatcgccgag atcgagcagc cgcagaaggt ggtgccggcg gtggtggaat tcgtcgacat
      961 cgcgggcctg gtcgcgggtg cgtcgaaggg cgaggggctg ggcaacaagt tcctcgccaa
     1021 catccgggag accgaagcca ttgtgcacgt ggtgcgctgc ttcgaagacg ccaacgtggt
     1081 gcacgtcgcg ggcaaggtcg acccggtgtc cgacatcgag accatcaaca ccgagctcgc
     1141 gctcgcggac ctcgattcgg tagagaagca gatcgccaag cacgccaagc tcgcgcagac
     1201 gaccggcgac aaggaaacgg ttcgcctggt ctcggccctc accaaagtga gagacgtgct
     1261 cgacgccggg cggccggcgc gcgtcgccga gctgtcgaag gaagagcgcg ccgtcgtcgc
     1321 gcccctgttc ctgctgacga tgaagccgac cctgtacgtg gccaacatcg ccgaagaggg
     1381 atccggcgcg ctcctcgaaa aagtaagagc gcatgcggcg aaggaaggag cgcccgtggt
     1441 cccggtcagc gcggcgatcg aatctcagat cgccgacatg tccgacgaag acaagcagct
     1501 gttcctcgag gacatcggaa tggacgagcc ggggctcaac cgggtgatcc gcgccgccta
     1561 cgcgatcctc gggctgcaaa cctacttcac tgcggggccg aaggaagtgc gcgcctggac
     1621 ggtgcgcgct ggcgccaccg cgccgcaggc ggccggcgtg atccacaccg acttcgagcg
     1681 cggcttcatc cgcgccgagg tcgcttccta cgacgactac gtcgcctgcg gcggcgagca
     1741 gggagcgaag gaggcgggta agctgcgcct cgaagggaag gaatacaccg tgcgcgacgg
     1801 ggatgtcatc cactttcgct tcaacgtcta acagcgttcg gatttttcaa tctcgtgagc
     1861 aaccgataac tcagggggct gcatgaagtc agtgctcttg gtgttcgctg ctggcgggct
     1921 gggatgcgtc gctcgataca agctcggcgc cctggtgctt caccacacga tagcttggaa
     1981 gtttccggcg ggaacatttg ccgtcaacgt gatcggctgc ctggttgcgg ggctgctcat
     2041 cggcttggcg gaaaaccacg gtttccttac ttcggaagtc cgtctgttgc tgttcaccgg
     2101 tttcttgggg ggctttacga cgttctcggc cttcggcgtt gaaaccgtcg ccttgatgca
     2161 gcgtggcgac tttgccgtcg ccgcctccga cgtcgcggcg agcgttgtct tcggaatcgt
     2221 ggctctctgg ggcgctgtca agctggccgg tctggggagc ggatagctgc cgcatctcac
     2281 agcgccgcgc tcgcgcggct atcatccgcc tgcatccgaa aggattgagg ggaatcctat
     2341 acatgaacct gcgccgtctg cgtctggtga cgggcctgat cctgatggcg ttcgtgatcg
     2401 gccacatggc caacctgatg ctcggcatgc attcgctcgc cgcgatggag agcgggcggc
     2461 cgtggctgat gggaccgtgg cgcagcatcg ccggccaggg gctgctggtg tgctccgcgc
     2521 tgatccacct ggcgctcggc gtgtacgcca tcgcagcgcg ccgctcgctc gcgattagca
     2581 agaccgacgt ggtgcagctt tgcctcgggc tcgcgacgcc gccgctcctg ctcaaccatg
     2641 cggtcgtcat gcacatggcc ggcgaggtga gcccgaattt cgatgccacc ttcggccaga
     2701 tgctggcggt ctactggagc ttctcgccgg gctatgcgtt ccagcagcta ggcgtggtga
     2761 tggtggtctg gatccacgcc gcggtcgggc tctattcgtg gctcgtgctg aagccggtct
     2821 ggaagcgcgt cggcggcctc atccttccgc tgctgttcgc ggtgccgatc ctggcgctgc
     2881 tcggtttcgc tgaagcgggc cgggaggtac tggagaagct ggcgaccgat gcggcgtggc
     2941 gcgagcacat caccgccaac ctgcggcgcg tcgtcgccgt gaccgatccg ctgaatgcca
     3001 tgcagtcgag gctgctctgg ggttacggaa tgctcgtgct cgtcgcgctc ggcgtgttcg
     3061 gcgcgcgtgt tctgcgcgag cgcctgcagc gcgtgacgat cggctacgac ggcggcgtgt
     3121 cggcgcaggg ccggcgcggg ctgtccatcc tcgagctctc gcgcctgaac gacgtgccgc
     3181 acgcgcacgt gtgctccggc cgcggccgct gcggcacctg ccgcgtgcgc atcgaatccg
     3241 gcgccgggca gctgacgccg atgaccgaag ccgagcgcgg cacgctcgcc cgggtcggtg
     3301 ccggcgcagg cgagcggctc gcctgccagg tccgcgtgac cggctcgggc gtggtcgcgg
     3361 cgcgcgtgct gccgccctat gccgatgcct cggcggcgcg cctgccgcac gaatgggtcg
     3421 cgccttgagc cagacgctgg ccggctggtc ggtacaggcg ctcgcggatg cggcgacgag
     3481 gatggaaacg ctgcgcgtcg acgtcctgcg ggcgccggag gagctgcgcg cgctggcggc
     3541 ggcaacgcac gcggcgctca tgagcgcgag cggcgtcgcg gctttcacct acgtcgtgat
     3601 cctgctgctt gccggtatcg gcgtcgagtg gctgttctgg acctacgcct actcgccgtt
     3661 gcgcgcgatc cagtcgagcg tccccgaatc gccgcggcag gcgctgcgcc tcgcgctgcg
     3721 gcgcctggtg ctcgtcgtct cggggctgct gctgttcacc gtcgccgcca tcggcgcgtc
     3781 ggcggcgttc tcgtggcctt ccggcgtgca ggagctcgtc gtcgcggcga cgctgctcgt
     3841 gctggtgctg cgcctcgcct gggttctcat ctgcgccgtg ctcgctcccg gccggccgcg
     3901 cctgagactg gtacccgtag cgaacgagcg cgccggctgg ctcgccgcgg cggcgatgac
     3961 ggtggtcttc ctgctggcgc tcgggagctt cgttcccaac gtgatggagc gcatcggcgg
     4021 cgccggccac gcggcgggcg ccctgcgcct gctcacgacg accgccgcgg cctttctcct
     4081 cgtcgtcgtc gcgttcgcgt tcttcggcgc gcgccgcggg ccgcggtttc cgcgatcgtt
     4141 cgtgctcgcg ctgatggtcg tcgtcgtcta cggcatctgg ctgatgaacg ccagggcggc
     4201 cacggtcgcg gcgatcgtcg ccgtcgtcat cgcgctgcag atcggcctgc gcgagctggt
     4261 gttcttcttc tggccggacg aggacgaccg cgtggctccg agcatcgtcc tgtccgcggc
     4321 gcgcttcgtc gtcgtgctgc tcgggcttgg cgccggcgcg ctggtgatcg aagcgccggt
     4381 gctggcgctc gcggcggccg agagcccgat gcttcggctg gtcctgcggc tgctcggcgt
     4441 ggcgacgctc gcgctactga cgcacgtgct ttggatcacg gtgcgcaccg ctgtcgacca
     4501 gcgcctcaag cggatccggc cggccggccc gcactacgag cccgacgagc actccaggct
     4561 gctcaccctg ctgccgctgc tgcgtgtcac cgccgcggtg ctgctcgtcg tcatgctggt
     4621 catttcttcg ctttgggcgc tcggcatcga gatcacgccg gtgctcgcgg gcgcgggcgt
     4681 cgtcgggctc gcgatcggct tcggcgccca ggcgctggtg cgcgatgtga tcgccggcgt
     4741 cttctacctc gccgaggatg ccttccgcat cggcgaatac atcgaggcgg gcggaaacac
     4801 gaagggcgcc gtggagcgca tcacgctgcg tacggtggcg ctgcgccacc acaacgggcc
     4861 gctgcacttc gtgccctacg gcgcgctcgg gaccgtgcgc aacacctcgc gcgactgggt
     4921 gatcgagaag ttcaacctgc cgctgccgat cgacgtggac agcgagaagg tacgcaagct
     4981 gatcaagaag gtcggcgaag agatgttggc cgaccccgag atcgggcgcc tgatcatgga
     5041 gcccctcaag ggcaagctgt accgcgtaga tcccggcgtg aagatcttcc ggtgcaagtt
     5101 ccgcaccgcg ccgggcaagc agttcgacgt gcgcgcgcag gcctacaagc gcatcgaggc
     5161 cgcgctcagg aagatggaag tgcgcttcgc cgacggccgg ctgccggtcc tgacaaacgc
     5221 atgagtgaaa cccgtttcga tgtggcgaag gccgcggatc tgccgcggct ggtcgagctc
     5281 ctcagactgc tgttcgaatc ggaagccgag ttttccgccg acgccgaaaa gcagcgagct
     5341 gcgctgcagg cgatcctcgc ggaccctgcg aagggccgga tcttcgtcgc gcgcgatggg
     5401 cgcgaagtga tcgccatggc gagcctgctc tatacgatca gcaccgccga gggcggcaag
     5461 gcggcgctgt tcgaggacct ggtcacggcg cccgagcatc gcaagcgcgg catcggcgcc
     5521 gcgctcctca agcacgtgat cgagcaggcg cgcgccgacg gcgtgctgcg catcaccctc
     5581 ttgacggata tgcaaaacga gcgcgcgcag gcgatgtatc ggcgcgtagg attcgtcggc
     5641 tcgccgatga aaccgatgcg gctgaaactc aagaaaggag cgaaatgaga tttctcgtcc
     5701 tggccgtcgc gctcgctgct gcgccggttc atgcccagaa gagcgacgac ccgcagatca
     5761 agcgcgggcg ctacctcgtg cagatcggcg gctgcaacga ctgcca
//
LOCUS       DATBSZ010000159        26001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2075217, whole genome shotgun sequence.
ACCESSION   DATBSZ010000159 DATBSZ010000000
VERSION     DATBSZ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 26001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26001
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075217"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /gene="hflX"
                     /locus_tag="VIG70_10440"
     CDS             complement(<1..737)
                     /gene="hflX"
                     /locus_tag="VIG70_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY8251015.1"
                     /translation="MRVIVAAVQRPSVSDAEFDASLTELRQLAKTLSFEVVATFTQKR
                     AGFDAAAYFGTGKREELKEKILENGAELILIDHEISPSQAFNLAKEVGGEVMDRTMVI
                     LEIFHRHARSKFARAQVEIARLGYMGPRLREAAKQAGPKDRARSGTGGRSGGESHGEL
                     DRRKIRDRIAELQEDLDAMVLERKTQRARRQERQGLARVALVGYTNAGKSTLMRALTG
                     SQVLVANKLFATLDTTVRALHPETVPRV"
     gene            complement(741..1757)
                     /locus_tag="VIG70_10445"
     CDS             complement(741..1757)
                     /locus_tag="VIG70_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY8251016.1"
                     /translation="MKRFLLLAGVPAVAVAAGLALWLQGGRYEETENAYVKAHMIAVT
                     ADVSGRVMEVGVRDNEPVAAGALLFRIDPVPFELAVVRAEAQMGVVRTELEGLRAEHR
                     VALADATEAEERIGFLARQLERQSKLKERGMGLESSYDEARHNLEAARRRLESSREKA
                     ARVLAALGGDAKLPVEGHPSFLAARAARDAGRDELSRVRVLAPAAGIVSNMRLQPGER
                     VERGVPVFSLIESRPVWIEANFKETQLTHMREGQEVTVVADAYPDAKWRGRVSTIAPA
                     TKAEFALLPAQNSSGNWVKVVQRVPVLIALDSFPDGQTPLRAGMTVTARVDTGHVRSL
                     SALR"
     gene            complement(1754..2194)
                     /locus_tag="VIG70_10450"
     CDS             complement(1754..2194)
                     /locus_tag="VIG70_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8251017.1"
                     /translation="MIREDLSRNFGYLVNDVARLMRTVYDRRVRSLGMTRSQWWVLNH
                     LFRHPGATQSELAAILEVERATLGRLLDRLEAKGWVRREDDARDRRAWRVHLAAAAEP
                     AMRKLRKQAFGLRRDALAGLNAADRERFVDTLLAIKANLSRLKR"
     gene            2237..3718
                     /locus_tag="VIG70_10455"
     CDS             2237..3718
                     /locus_tag="VIG70_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY8251018.1"
                     /translation="MTPSLQRAWVLATCTGVTFLYSMSLTAVNVSLPQIQGALSATPD
                     QIAWVITSNLVATAVMTPLTGWLAARFGARLLCVACVAGFTVASAACGLAGSIEALVA
                     WRTLQGASGAPLVPMSQAIMLASFPRERHGAVISIFGVASVLGPVVGPVLGGALAEAY
                     DWRWVFYMMVPFSLAALAACWLFIRDRESPQAVRLDWTGFLMLSAALAGLQLMLDRGE
                     RAGWFSSPEIVGWAALAALGLYLFIAHTLTHARPFLDPRLLRERNFALGLVLIFVFGM
                     LNFTPMTMLPPMLQGVAGYPDSVIGWVLGARGLGTMAGFSLMIYASRFDPRFMIALGF
                     LLQALAGWQLAQLDPGFGVHDVFWPMALQGLGVGLLWVPITMVAFSAIEGERMAEGTA
                     VYHMLRNIGSSVHISLSTVLVVHMTQASHAELTPNVSRYNEALSLPWVRGNLDLGDPS
                     SLAAIEAEIARQASMLGYLDSFWAFSATALVVLPLILLVRWKR"
     gene            3751..4239
                     /locus_tag="VIG70_10460"
     CDS             3751..4239
                     /locus_tag="VIG70_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8251019.1"
                     /translation="MPLTELNHFLLVAKNLERTKKFYQDVLGMELADRPDFGFPGYWL
                     KIGDDICVHLASQDPNKTRDMFLLKKHPKGTNGSGQVDHIAFLAKDPYGVRSRIQKNK
                     VEMHFRSFPDAKLFQIFLKDPDDVTIELNFLGEVIDEKQWKGKGSASAVTLDGGKAKR
                     QN"
     gene            4236..4790
                     /locus_tag="VIG70_10465"
     CDS             4236..4790
                     /locus_tag="VIG70_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8251020.1"
                     /translation="MIRALAVFLLLGASSAQASEALWTLLKGGGQVVLIRHTITTPGV
                     GDPPGFRLDDCATQRNLTDEGRGHAKRIGEAFRARGIPVERVLSSPWCRCIETAKLAF
                     GRTDGLSEALGNLFGRPENRAKQVEQMRALASTRPKSGNRVLVSHGSTIVALTDIAPA
                     TGEMVIVTPQGNGKFAVAGRLGVN"
     gene            4792..5931
                     /locus_tag="VIG70_10470"
     CDS             4792..5931
                     /locus_tag="VIG70_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEY8251021.1"
                     /translation="MLFDEIRIRDVRLRNRIVVSPMCQYSAQDGHVADWHLVHLGKFA
                     QGGAAAVFVEATAVEKRGRITHGDTGIWDDAHIAGLQRIAAFVKSQGAVPAIQLAHAG
                     RKASMARPWHGNGPLTQEDLQRGDKPWGIVAPTDAAIGEEWLRPRAFEKGDFQVLLNA
                     YRNAVRRAHAAGFEILELHAAHGYLLHTFLSPISNTRKDEYGGSLENRMRFPLEVAKE
                     VRNAWPAGKPLFMRVSSIDDVEGGWGVDDTVLFSRQLKAIGVDVVDCSSGGILGSATA
                     ATKTILQRVPGFQIPFAERVKKEAGLMTMAVGLILTPQQAEEALQAGRADLIAIGREA
                     LYDPNWPLHAALALGADPEFAQWPQQYGWWLTRRESLLRKVGFRR"
     gene            5928..6497
                     /locus_tag="VIG70_10475"
     CDS             5928..6497
                     /locus_tag="VIG70_10475"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8251022.1"
                     /translation="MKTITLSKQDMEKRIARFKRLSPLPIQQSDIPLAARDVVYSRKL
                     LSVVGLENASTPVNQGAPIIGAAGMTLTLAVCPPGQGPGLHAHRQTYETFTVMKGRFE
                     VRWNDDGSGSAILELYDTVSFPPGVCRAFRNVGNEEGILQVIITGGVHDMTDIDFSEN
                     AKKEIEAVRPGLSAEFEKAGFTFSIPFRS"
     gene            complement(6469..6822)
                     /locus_tag="VIG70_10480"
     CDS             complement(6469..6822)
                     /locus_tag="VIG70_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY8251023.1"
                     /translation="MNTTYAQAEPRRAEVDQFAGPTLIEFGSPWCGYCRAAQPLIAEA
                     LAGHAGIRHLKIADSSGKRLGRSFRIKLWPTLIFLRDGKELARLVRPRDAKSIGDAIT
                     QLNGNRLSFGTESKR"
     gene            complement(6819..7202)
                     /locus_tag="VIG70_10485"
     CDS             complement(6819..7202)
                     /locus_tag="VIG70_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251024.1"
                     /translation="MKSARSTRAASRTRFLAALVAGGVAACAANHELTAARDSWRGAT
                     YEQVVAAWGPPAQSAKDSHTWLSEDRAPQAQRSGGGAGGVVFGAPEGAAARCDRTLTF
                     RDARLLDQSWKGNGEFCKRFGRPRR"
     gene            complement(7168..7554)
                     /locus_tag="VIG70_10490"
     CDS             complement(7168..7554)
                     /locus_tag="VIG70_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251025.1"
                     /translation="MAFLAACATTEKAKESWTGATYDDAVRAWGPPVRSGKLADGAEV
                     HTWWSETGPTYRSGPSVGFGIGGFGGGGRGGGVGVGVGASVPVGEGSVAPPARCERTL
                     TFRDGRLVEQSWIGPDEICSVYARSK"
     gene            complement(7568..7999)
                     /locus_tag="VIG70_10495"
     CDS             complement(7568..7999)
                     /locus_tag="VIG70_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8251026.1"
                     /translation="MRVSEAMTRDVRIANPGQSIRDVAKIMAEIDAGVMPVGENDRLV
                     GMITDRDIAIRAIAKGKGPDTPVREVMSKDVKYCYEDEDLEHVARNMGDIQVRRLPVV
                     SRDKRLVGIVSLGDVAQNEDSETTGETTAGVSQRGGQHRQT"
     gene            complement(8016..8396)
                     /locus_tag="VIG70_10500"
     CDS             complement(8016..8396)
                     /locus_tag="VIG70_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251027.1"
                     /translation="MARVVDGQVEMLLKREARSRLPLVRQILIYLDPFALFKDASQGP
                     APARANALSYNRTMRWMLLPYIRRWIVIAASLALLISPAEAAAAQQAMFAIPMAAVAV
                     GCCIAATVAAVTVAVYLLLGQRAV"
     gene            complement(8474..9082)
                     /locus_tag="VIG70_10505"
     CDS             complement(8474..9082)
                     /locus_tag="VIG70_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEY8251028.1"
                     /translation="MKFSPIAVAVAAAMLSFGAVAQEKKQESQAGQTPSMSQEQKPST
                     QAQGSASGSASTSGSASSPAASGSTSASTEKKSEGSAAAGGSSMSHGSHDAETIKQVQ
                     TKLNIQADGKMGPETQKALKEFQTSKGINASGQLDQQTLAALGISSAAGGASAKAEKK
                     AEGSAAAGASTPAAGASTTEQKPQQPPAPPAKVDTKPGEKKY"
     gene            9196..9708
                     /locus_tag="VIG70_10510"
     CDS             9196..9708
                     /locus_tag="VIG70_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251029.1"
                     /translation="MGLAPQTKILVAMENEDRMVRVLDGHYLAVVKTCDEARLLLERE
                     TFGIVILGVHFDESQMFSLLGDIRAHAQYRKVPILCVLGGRGRILTDVAVEGLDHAVK
                     AMTANGFLNLENFPDDEKGNARLRRIVDYLILIDGDLQQLPEGSENGKPVPGHLLGEP
                     RRRASDPQTH"
     gene            complement(9718..10155)
                     /locus_tag="VIG70_10515"
     CDS             complement(9718..10155)
                     /locus_tag="VIG70_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEY8251030.1"
                     /translation="MRTKLLNDNGERVFAVIFETGDDPVAGLTRFAEERKLGASAFTA
                     IGAFSEATLGYFDWDKKDYERIPVKEQVEVLALVGDIALQDGKPKLHAHVVLGRRDGS
                     ACGGHLLAARVRPTLEVILTESPAHLRRRHDPVSGLALIDLPA"
     gene            complement(10137..10463)
                     /locus_tag="VIG70_10520"
     CDS             complement(10137..10463)
                     /locus_tag="VIG70_10520"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEY8251031.1"
                     /translation="MKRILLTVCLILAPLGVFAASPFTSLHPEEAIPDPEQPVSTGPH
                     SGFISRVQERLRELGFDAGPVNGDFGEKTQAALAQFQLSRTIPASGQMDKETRAELGV
                     TCEQSC"
     gene            complement(10498..10947)
                     /locus_tag="VIG70_10525"
     CDS             complement(10498..10947)
                     /locus_tag="VIG70_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEY8251032.1"
                     /translation="MRTPASIARHPIHPMLVPIPIGLWIFSLVCDLIHAGGSANPAWT
                     TVAFYCMGGGIIGALLAAVPGLIDLVSLPAGPRGTALKHMAINLVVVALYAINFWMRL
                     SEPGNPGKLVWLSLVSVGLLAISGWLGGKMVFEHGIAVDTPPDQQRR"
     gene            11086..11409
                     /locus_tag="VIG70_10530"
     CDS             11086..11409
                     /locus_tag="VIG70_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEY8251033.1"
                     /translation="MRRNTLIAVSIAAAFALPLTVQAGGDKASQGASSSAATGAEKMF
                     QSLDKNKDGSLSRDEVKGSPHDKDFATLDKNKDGKLTRQEHAAAAEHAGDKAAAGGTS
                     SKPKY"
     gene            complement(11406..11627)
                     /locus_tag="VIG70_10535"
     CDS             complement(11406..11627)
                     /locus_tag="VIG70_10535"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEY8251034.1"
                     /translation="MARIVKRTPVEPTKFVIEGKEQWFCKCGLSKNQPYCDSSHKLTR
                     GEEPGKLYWYDDGGKRHEVAGDFPGIRTF"
     gene            11885..12121
                     /locus_tag="VIG70_10540"
     CDS             11885..12121
                     /locus_tag="VIG70_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251035.1"
                     /translation="MSLFWAVVLWLALPRRRTMLWALLASAAIALIDLRLIAPAFFPE
                     VAALAFWPQFADHLMWGACYSVGLSLGRPRATRP"
     gene            complement(12073..13221)
                     /locus_tag="VIG70_10545"
     CDS             complement(12073..13221)
                     /locus_tag="VIG70_10545"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8251036.1"
                     /translation="MTGIHEKLWASAEALLPKLKERAARCEELRRLPDDTLRDFHDAQ
                     LFRAHQPRRVGGAELEFETVVTFGALLARACASTSWNWVNFTIHHMMLGMFPPQAQDE
                     IWGKSPDTLIASSSVFPAGKARKVEGGYVVSGRWPFSSGVDPSEWNMLAGLARLDDNL
                     PPEPRIFLLHRSQYKVIDNWFAGGLRGTGSKDVEAGEVFVPEHLTLAVADTKGGPTPG
                     SAVNPGPLYRMPVFALFPFMLSGVALGIAEGLIDDYKPASGRMTGARVAEIQSTQIRL
                     GEATAYARASRLVQLANCREAQEKLPDLKTKARFRLEGAYSVEWAVRAVDVMFGLYGA
                     SGLYESGPVARAFRDAHAVKQHFSFNTDIAGTTYGRVALGLPSDNPTL"
     gene            complement(13184..14065)
                     /locus_tag="VIG70_10550"
     CDS             complement(13184..14065)
                     /locus_tag="VIG70_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY8251037.1"
                     /translation="MKLPKDRIAYSAIVDRPALKLPNGARVAVWTIVNVEEWSIERAM
                     PRTVLPPPYGQPLLPDLPNWSWHEYGMRVGFWRFLEALQKYSIKATLAINGSVVKSYP
                     RVSEAALKAGWEFMGHGYIQRPMHHVEDQREAIADTVQAIREFTGKPPRGWESPGLTE
                     TYDTIDWLAEAGIEYVADWVLDDQPCTIQTAHGPIVSVPYTVEMNDIAMMVLQNHPSE
                     EWLRRGVDQFDRLYAEGEKSARVMAISVHPYISGVPHRIAYLERLYEYILQRPGALIW
                     TGEQILDWYSREALGKR"
     gene            14108..15064
                     /locus_tag="VIG70_10555"
     CDS             14108..15064
                     /locus_tag="VIG70_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY8251038.1"
                     /translation="MLNGMRLLCVAAAAFATGTQAQTVGFATLPPGSILHAQASVIAK
                     VVQDNSKLQVRVVGYGGDTGILEAVNSKQSDFWILDVGESADALHGRGTWKGNPKPAL
                     RTALTMFPFQMAFWVRKDSSIQSIADLKGKRVPAEWVQQTGVIPHTTAVLAAGGITYN
                     DVVKVPEVNVVRAADDFKAGKLDLLFFAVGAPKVAEVSAAVGGLRLLPMNPLPDSEKR
                     MKAVRPEYYFTTVNPAPHIAGVDKPSPVQTIDVVVGVGAHVSDEVVYNFVKAVHGNKA
                     GMVAGHPNFNAFQEKAAGKPQPSLPHHPAAIKFFKEAGIWRG"
     gene            15115..16932
                     /locus_tag="VIG70_10560"
     CDS             15115..16932
                     /locus_tag="VIG70_10560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEY8251039.1"
                     /translation="MDLFRPIGLVLFSEQFAAAAYGIGLSLTFLRFRFSGKSSDVPWY
                     DWLLAAASFAACGYTAVKFPKLTALASTTTPEVLILSVLLTALAIEAVRRTIGWSLVI
                     ILLALAGYVLIGHLVPGQLQTREVGVGQMLVYLNMDNNGLLGLVLQITVIVIIPFVLM
                     GQLLMRSGGSSFFNDAAIALMGRYRGGAAKMAVVGSSLFGMVSGIAAANTVAVGVVTI
                     PLMKRSGMSAKLAGAVEACASNGGQLMPPVMGAVAFVMADFLQIPYTEVAIAAILPSV
                     MYYAALFIQADLEAARYGFGKVEEDKIPRIGRVLAQGWLFLAPFAVLVYTMFWLNLEP
                     EYAAMAASATIIVLGFAFGYAGSRMRLADIWHAIRETGTSLCEILVISAAAGFIMGLF
                     QVSGLAFAFAAYLVQLGSESLVLLLVLAAVVSIILGMGLPTIGVYVMLAILVAPALVK
                     VGVPPLAAHLFILYFGMMSLITPPVAPAAYVAAAIAGAPSMATGWTAMRFGWSSYIVP
                     FLFVYSPAILMQGSIVDIVTVTITSLFGIWLICAAMTGYFVKTMNWKTRCAFALAGIM
                     MLSPHQVSELMLWINILGAAAGIALIAYELRARRAYVRA"
     gene            16919..17965
                     /locus_tag="VIG70_10565"
     CDS             16919..17965
                     /locus_tag="VIG70_10565"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY8251040.1"
                     /translation="MYVRNAWYVAAWDHEVTREMRRRILLDEPVLLFRKDDGTPVALE
                     DRCCHRQAPLSMGKLVGNIVQCPYHGLQFDTTGKCVKVPSQDMVPASARVRSYPIVEK
                     NHWLWIWMGDPAKANPELIEDFHWLDDPGWRFGGSYLHVDAHYLLVVENLLDTTHLPF
                     LHPNTLGTDTFARSEFDVKREGDRIIVSRWVMNELPAPFHKQMGGFPDGLKVDRWQFT
                     HFGPPCFVKLDVGSSPAGAGARQGDRSKGVNMWNLNAITPETEKSAHYFFAQAYNFKL
                     NEQWVSDMLRTQVHSIFLEDMAIIKAQQQNMDLGTSPVTSLGQDKAWVAMRGIVQRLI
                     NEEQQATRPVAVSA"
     gene            complement(17994..18392)
                     /locus_tag="VIG70_10570"
     CDS             complement(17994..18392)
                     /locus_tag="VIG70_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY8251041.1"
                     /translation="MSALIIDTNAYAGLLGGEPKTVQFLRAAGEIYLPLIVLGELLAG
                     FAAGTRAQKNREELTRFMASTRVQVLKPDEKTAQHYGDVFVTLRRQGTPIPTNDLWIA
                     ALVRQHRMPLLSFDAHFAAVPGLALINPSV"
     gene            complement(18389..18724)
                     /locus_tag="VIG70_10575"
     CDS             complement(18389..18724)
                     /locus_tag="VIG70_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251042.1"
                     /translation="MLSTAITGRSSMPNISLRGLDASTLSRIKSRARRRNISVNRLIV
                     ETLQREYAAGEPASDDLDALAGAWSKTEAAAFLAAVAPFAEIETGLWAGEPKAAYRAR
                     PAAGGKARK"
     gene            complement(18751..19905)
                     /locus_tag="VIG70_10580"
     CDS             complement(18751..19905)
                     /locus_tag="VIG70_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEY8251043.1"
                     /translation="MTTEWAAFWGVVAIALAVDLRFSKKSDPKSAAAWSAVWIGLGLG
                     FGGWIALRLGTEAGITYLTAYVLEKSLSVDNLFVFILIFSLTGIPASLQHRALFWGIV
                     GALAMRAALIAVGVEILERFQWAVYPLAALLAYAAYRMLRGEERQERFAERQCALCES
                     WLARFIPIEPRLHGSRFVVRLDGRLMATPLLVALTVIETTDLLFAVDSIPAVFAVTRD
                     PFLVYTSNVFAMLGLRSLYFLLAGVIRKLRFLKVGLAVMLFMAAAKLAVGDLYHVPPL
                     ASLAAIVAILVGSIAASLLFPAAAKACTHRDQIAGVAPSSNGCEECLRMGERWVNLRM
                     CLKCGNVGCCDSSKNRHATAHFHATGHPVMRSIEPGESWKWCYVDRVKLD"
     gene            complement(19902..20744)
                     /locus_tag="VIG70_10585"
     CDS             complement(19902..20744)
                     /locus_tag="VIG70_10585"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8251044.1"
                     /translation="MNVGFVGLGQMGRPMVERLRGAGHSLKVYNRTRHSAEVLEHPDA
                     VLDAEIVITMLADDAAVRAVWLESGLASRLPRDAIHLNMSTVSMDIARKLTAVHQKSL
                     YVAAPVFGRPPLAAQGQLDIIAAGPKAALERCEPLFKILGKQTFVVGEEPAKANAVKI
                     ARNFLLATVIESLGEAFALVRKCGIEPQRFLEVLSSTSLGSPAYRNYGKMIVEEAWTP
                     AQFAMPLGVKDVELALATATDVGMPLPSGEMIRRHLLEAIAAGRAHQDWAALAGKLAA
                     DAGL"
     gene            20820..21692
                     /locus_tag="VIG70_10590"
     CDS             20820..21692
                     /locus_tag="VIG70_10590"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY8251045.1"
                     /translation="MVVPWPPAGVTDVLTRALVQAMSESTGQQFVVENRPGAGGTLGL
                     APVAKSAPDGYTLVASDVPSHAISATLYAKLPYDVLHDFEPIAMIAGSPMVLATNPAL
                     GVRNLKEFILVARAKSPPMSYGSSGNGSITHLAIERLKRDAGMDLVHVPYKGTVPAIT
                     SVLSGDTALAFGTIPGVMPHAKAGKLVPLGSSFGKRFSQIQEVPAIAETIPGYDMGFY
                     TALFAPAKTPPEVIQKVFLEVTKALAQPKVKEIFANSAAEPGTMTSAELRQYLAAEVK
                     AWGDVVRSVGLKVD"
     gene            21735..21962
                     /locus_tag="VIG70_10595"
     CDS             21735..21962
                     /locus_tag="VIG70_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251046.1"
                     /translation="MTQPKRKKQRHKQPPTGSTTNASDAVDDIELPPNPSAEEKLDAG
                     VQETFPASDPVSIAQPSETAYEREKRLKRSR"
     gene            complement(21959..23182)
                     /locus_tag="VIG70_10600"
     CDS             complement(21959..23182)
                     /locus_tag="VIG70_10600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8251047.1"
                     /translation="MGADSHSRGLFTRVTLPLAVFGLINQAARTVMAVIGPVLAVEFA
                     LSASELGLLAACMLAAYAAVQLPGGMALDRVGARNVQAALALVTAAGFALFALADSFA
                     TLVIARVIVGVGISAGLMAIIKANAQWFVPAKVALVTGIAVAIGGLGSVASTVPVAAA
                     LPALGWRGVLWVLCGLSAATALWIFASVPDRRGSYSGGLKTELGIMASILGSRVFWRF
                     APAVAMLSVLNFAYLGLWAGPWLRDVAGYDSRQSASTLLAYTVTMMVGALVVGAATSR
                     AKARGYPPMLVTLACTAGQAAAQIGLVLQPAAPLVVGGLWMLFAFCVAGATAGYITVG
                     QMFPPEQTARVATAVNTLTLAGAFVLQSATGWVLDFWPRTASGGWDPQGYSTALAFSL
                     LLQLLMAARLLGGKR"
     gene            23181..23573
                     /locus_tag="VIG70_10605"
     CDS             23181..23573
                     /locus_tag="VIG70_10605"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8251048.1"
                     /translation="MERIVVHPPRPGLDMAAGCGWVQSTAIRAGDFVFVSGIVALDPE
                     SGERLNGTLTSEAHRVFLNLKLVLEAAGSSLERLVQVRAMIYDRIEYDALNRVYRQYV
                     PHGPPARTVWSVRIIDAFKVQLDAVAVV"
     gene            23589..23990
                     /locus_tag="VIG70_10610"
     CDS             23589..23990
                     /locus_tag="VIG70_10610"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8251049.1"
                     /translation="MSVAREIILPKNERLNVSRNFNLPHSPGVRAGDFIFLSGMLSTD
                     PQTGERSPGTMAHETRQTLTNMRHLLESAGSSLERVVKINVLLYDMLEFENMNAVYRQ
                     FFPVDPPARTTCGVQLSAGMKIEIECIALAN"
     gene            complement(24073..25032)
                     /locus_tag="VIG70_10615"
     CDS             complement(24073..25032)
                     /locus_tag="VIG70_10615"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HEY8251050.1"
                     /translation="MTNTVPTIDISRPGIAEAVARTCEQTGFLIISGHGFPLTLLERA
                     QRELFAFFDLPEATKNRWHPSGPSRQRGYHGFATRGLAYTLGQKTPPDLRETVFLGPV
                     DDHRAYYAGMPEAATSYAPNLIPDEPAGLAGTLVALYREYERLARDLMRVFAAALRLP
                     GGFFDGTLDRHFSIMSCHHYPVLEKPPLPGQLRTGAHTDYGAMTILAATDAEGGLEVR
                     LPDGSWSGVRPRVGEFVVNLGDMMARWTNGSWASTLHRVANPPLGSAQSRRLSIGMFV
                     HPSYDQRIECVPTCLAPGEKPRYATISAGEHIKRKIERSHEAA"
     gene            complement(25029..25922)
                     /locus_tag="VIG70_10620"
     CDS             complement(25029..25922)
                     /locus_tag="VIG70_10620"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251051.1"
                     /translation="MRITLCENMRAVPYVPFYLALAGGYWESEGLDIRHVVSPSTSRT
                     ATALLDGTVDVSWGGPMRVMMHHDADPRCPLVCFGQVVARDPFLLVGRTQKPGFRWRD
                     LCGLRVAPAGDVPTPWMTFQDDLQRAGLDPAAIAGRRVRPMARNLEAFRRGTVDVVQV
                     FEPYADRLVREGRGHVWHRFSERGDIVYTTFYATRRVTRARREACRRLVRGMARAQAA
                     LFAATPGALADAVSRFLPKLSREALARIIGAYRASGLWAATPDLPPAPFLRLKAALVS
                     GGLIRSDPPYERIVDAELSRK"
BASE COUNT         4233 a         8836 c         8643 g         4289 t
ORIGIN      
        1 acgcgcggca cggtctccgg gtgcagggcg cgcaccgtgg tgtcgagcgt cgcgaacagc
       61 ttgttcgcca ccagcacctg gctcccggtg agcgcgcgca tcagggtgga tttgccggcg
      121 ttggtgtagc cgacgagcgc cacgcgcgcg agcccctggc gctcctgccg ccgggcgcgc
      181 tgcgtcttgc gctcgaggac catcgcgtcg aggtcttcct gcagctcggc gatgcggtcg
      241 cggatcttgc gccggtcgag ctcgccgtgc gactcgccgc cgctgcgccc gccggtgccg
      301 ctgcgcgcgc ggtccttcgg gccggcctgc ttggcggcct cgcgcaggcg cggccccatg
      361 tagcccaggc gcgcgatctc gacctgcgcg cgcgcgaact tcgagcgcgc gtggcgatgg
      421 aagatctcga ggatcaccat ggtgcgatcc atcacctcgc cgccgacctc cttcgccagg
      481 ttgaaagcct gcgagggcga aatctcgtgg tcgatcagga tgagctcggc gccgttttcc
      541 aggatctttt ccttgagttc ttccctcttg ccggtcccga agtaggccgc cgcgtcgaag
      601 ccagcccgct tctgggtgaa cgtggcgacc acctcgaagc tgagcgtctt cgcgagctgg
      661 cgcagctccg tcagggaggc atcgaactcg gcgtcgctga cggagggtcg ctggaccgcg
      721 gccacgatga cgcgcatcgg ctagcgcaac gccgagaggc tgcgcacatg accggtgtcg
      781 acgcgcgccg tcacggtcat gccggcgcgc agcggggtct gaccgtcggg gaaagagtcg
      841 agggcgatga gcacggggac gcgttgtacc accttcaccc agttgccgct cgagttctgc
      901 gccggcagga gcgcgaactc ggccttggtg gccggagcga tggtgctgac gcggccgcgc
      961 cacttcgcat ccgggtaggc atcggcgacc accgtgactt cctggccttc gcgcatgtgg
     1021 gtgagctggg tttccttgaa gttggcttcg atccacaccg ggcgcgactc gatcaggctg
     1081 aagaccggga cgccgcgctc gacgcgctca ccgggctgca ggcgcatgtt gctgacgatg
     1141 ccggcggcgg gagcgagcac acgcacgcgc gagagctcgt cgcgcccggc gtcgcgcgcc
     1201 gcgcgcgcgg cgaggaagct cgggtggccc tcgaccggga gtttcgcgtc gccgcccagc
     1261 gcggcgagga cgcgcgccgc cttctcgcgc gagctttcca gccgccggcg cgccgcctcc
     1321 aggttgtggc gcgcctcgtc gtagctcgac tccaggccca tgccgcgctc cttgagcttc
     1381 gactgccgct cgagctggcg cgcgaggaag ccgatgcgct cctcggcctc ggtggcgtcg
     1441 gcgagcgcaa cgcggtgctc ggcgcgcagg ccctcgagct cggtgcgcac cacgcccatc
     1501 tgcgcctccg cacgcaccac cgcgagttcg aacggcaccg gatcgatgcg gaagaggagc
     1561 gcgcccgcgg ccaccggctc gttgtcgcgc acgccgacct ccatcacccg gccggacacg
     1621 tcggcggtga ccgcgatcat gtgcgccttc acgtaggcgt tctcggtctc ctcgtagcgc
     1681 ccgccctgca gccacagcgc cagccccgcc gccacggcga cggccggcac gcccgccagc
     1741 aggagaaagc gcttcatcgc ttcagacgcg aaaggttcgc cttgatggcg agcagcgtat
     1801 cgacgaagcg ctcgcgatcg gccgcgttca gcccggcgag cgcgtcgcgc cgcaggccga
     1861 aggcctgctt gcgcagcttg cgcatcgccg gctcggcggc ggcggcaagg tgcacgcgcc
     1921 aggcgcgccg gtcgcgcgcg tcgtcctcgc gccgcaccca gcccttcgcc tccagccggt
     1981 cgagcagccg gccgagggtg gcgcgctcca cctccaggat cgcggcgagc tccgactggg
     2041 tggcgccggg atggcggaaa aggtggttca gcacccacca ctgagagcgc gtcatgccca
     2101 ggctgcgcac gcgccggtcg tagaccgtgc gcatcaggcg cgcgacgtcg ttcaccaggt
     2161 agccgaagtt gcgcgacagg tcctcgcgga tcatggccgc gccagtttag gtgcctagct
     2221 tacaatgagc aaggcaatga ccccctccct gcagcgcgca tgggtgctgg cgacctgtac
     2281 gggggtgacg ttcctctatt cgatgagcct gacggcggtg aacgtgtcgc tgccgcagat
     2341 ccagggcgcg ctgtcggcga cgccggacca gatcgcctgg gtcatcacct ccaacctggt
     2401 cgccaccgcc gtgatgacgc cgctcaccgg ctggctcgcc gcgcgcttcg gcgcgcgcct
     2461 gctgtgcgtg gcgtgcgtcg cgggcttcac cgtcgcctct gccgcctgcg gcctggccgg
     2521 ctccatcgag gcgctggtgg cctggcgcac gctgcagggc gcctccggtg caccgctcgt
     2581 gcccatgtcg caggcgatca tgctggcgag ctttccccgc gagcgccacg gcgcggtgat
     2641 ctcgatcttc ggcgtcgcct cagtgctcgg cccggtggtc gggccggtgc tcggcggcgc
     2701 gctcgccgag gcttacgact ggcgctgggt gttctacatg atggtgccgt tcagcctggc
     2761 ggcgctcgcc gcgtgctggc tcttcatccg ggatcgcgag tcaccgcagg cggtgcgcct
     2821 ggactggacc ggctttctca tgctttcggc ggcgctcgcg ggcctgcagc tcatgctcga
     2881 ccgcggcgag cgcgccggct ggttctcttc cccggagatc gtcggctggg cggcgctcgc
     2941 ggcgctcggt ctgtacctct tcatcgcgca cacgctcacc catgcgcggc cgttcctcga
     3001 cccgcgcttg ctgcgcgagc gcaatttcgc cctcggcctg gtcctgatct tcgtcttcgg
     3061 catgctcaac ttcacgccca tgacgatgct gccaccgatg ctgcagggcg tggccggcta
     3121 cccggactcg gtcatcggct gggtgctcgg cgcgcgcggc ctgggcacga tggcgggctt
     3181 ctccctcatg atctacgcca gccggttcga cccgcgcttc atgatcgccc tggggttcct
     3241 gctccaggcg ctcgccggct ggcagctcgc gcagctcgac cccggcttcg gcgtccacga
     3301 cgtgttctgg ccgatggcgc tgcaggggct cggcgtcggc ctcctgtggg tgcccatcac
     3361 catggtggcc ttctccgcca tcgaaggcga gcgcatggcc gagggcacgg cggtctacca
     3421 catgctgcgc aacatcggca gcagcgtgca catctcgctc agcacggtgc tggtggtgca
     3481 catgacgcag gcgagtcacg ccgagctgac cccgaacgtc tcgcgctaca acgaggcgct
     3541 gtccctgccc tgggtgcgcg gcaacctgga cctcggcgac ccgagcagcc tggccgcgat
     3601 cgaggccgaa atcgcacgcc aggcctccat gctcggctac ctcgactcct tctgggcctt
     3661 ctcggcaacg gcgctggtgg tgctgccgct gattctgctg gtgcgctgga agcgctagca
     3721 tttagcattg gacaagcaag gagaaccccc atgcccctga ccgagctcaa ccattttctg
     3781 ctcgtcgcca agaacctcga gcgcaccaag aagttctacc aggacgtcct cggcatggag
     3841 ctcgccgacc ggccggattt cggctttccg ggctactggc tcaagatcgg cgacgacatc
     3901 tgcgtgcacc tggcgtcgca ggatccgaac aagacgcgcg acatgttcct gctgaagaag
     3961 catcccaagg gaaccaacgg ctccggccag gtcgatcaca tcgccttcct cgccaaggac
     4021 ccttacgggg tgcgcagtcg catccagaag aacaaggtcg aaatgcactt ccgcagcttc
     4081 ccggacgcca agctgttcca gatcttcctc aaggatcccg acgacgtcac gatcgagctc
     4141 aacttcctcg gcgaggtgat cgacgagaag cagtggaaag gcaagggctc ggcctccgcc
     4201 gtgacgctcg acggcggcaa ggcgaagcgc cagaattgat ccgcgccctc gcggttttcc
     4261 tcttgctggg agcttcttcc gcccaggcga gcgaggccct gtggacgttg ctcaagggcg
     4321 gcggccaggt ggtgctgatc cgccacacca tcaccacgcc cggcgtgggc gatccgcccg
     4381 gcttccggct cgatgactgc gcgacccagc gcaacctcac cgacgaggga cgaggacacg
     4441 cgaagcgcat cggcgaggct tttcgcgcgc gcggtattcc ggtcgagcgc gtgctctcga
     4501 gcccatggtg ccgctgcatc gagacggcga agctcgcatt cggcaggacc gacggcttgt
     4561 cggaggcgct cggcaacctg ttcggccgcc cggagaaccg ggccaagcag gtcgaacaga
     4621 tgagagccct ggcgagcacg cgtccgaaga gcggcaaccg ggttctcgtg tcgcacggct
     4681 ccaccatcgt cgcgctcacc gacatcgcgc ccgcgaccgg cgagatggtc atcgtcacgc
     4741 cgcagggcaa cggcaagttc gccgtcgccg gccggctggg agtgaactga aatgctgttc
     4801 gacgaaattc gaatccgcga cgtccggctc agaaatcgca tcgtggtgtc gccgatgtgc
     4861 cagtactcgg cgcaggacgg ccacgtcgcc gactggcacc tggtgcacct cggcaagttc
     4921 gcgcagggcg gcgccgccgc ggtgttcgtc gaggcgaccg cagtggaaaa gcgcggccgc
     4981 atcacgcacg gcgacaccgg gatctgggac gacgcgcata tcgccggcct gcaaaggatt
     5041 gccgcattcg tgaagtccca aggtgccgtg ccggcgatcc agctcgccca cgccgggcgc
     5101 aaggcgagca tggcgcgccc gtggcacggc aacggcccgc tcacccagga ggatctccag
     5161 cgcggcgaca agccctgggg catcgtcgcg ccgaccgacg cggcgatcgg cgaggaatgg
     5221 ctcaggccgc gcgctttcga gaagggcgac ttccaggtcc tgctgaacgc ctaccggaac
     5281 gcggtgcgca gggcgcatgc agctggcttc gagatcctcg agctgcacgc tgcgcatgga
     5341 tatctcctgc acacgttcct gtcgccgatt tcgaacaccc ggaaggacga atacggcggt
     5401 tcgctggaaa accgcatgcg cttcccgctg gaggtggcga aggaagtccg caatgcctgg
     5461 cccgccggca agccgctctt catgcgcgtc tcgtcgatcg acgacgtcga aggcggctgg
     5521 ggcgtcgatg acacggtctt gttctcaaga caattgaaag caataggcgt agatgtcgtg
     5581 gactgctcct ccggcggcat cctcggctcg gcgaccgcgg cgacgaagac gatcctgcaa
     5641 cgcgtgcccg gcttccagat cccttttgcc gagagggtga aaaaggaggc cggcctgatg
     5701 accatggcag tcggcctcat cctcacgccg cagcaggccg aggaagcgct gcaggccggc
     5761 cgcgccgacc tgatcgcgat cggccgcgag gcgctctacg acccgaactg gccgctgcac
     5821 gcggcgcttg cgctcggcgc cgatccggaa ttcgcgcagt ggccgcagca gtacggctgg
     5881 tggctgacgc gccgcgaaag cctgctcaga aaggtggggt tccgccgatg aagacgatca
     5941 cgctgtccaa acaagacatg gaaaagcgca tcgcgcgctt caagcgcctt tcgcctctcc
     6001 ccatccagca aagcgatatc ccgctggcgg cacgagacgt ggtctattca cgaaaactgc
     6061 tttcggtggt tggcctggaa aacgcgagca ccccggtcaa ccagggagcg ccgatcatcg
     6121 gcgcggccgg aatgaccctg acgctcgcgg tgtgcccgcc gggccagggg cccggcctgc
     6181 acgcgcaccg ccagacctac gagaccttca ccgtgatgaa gggccgcttc gaggtgcgct
     6241 ggaacgacga cggctcgggg agcgcgatcc tcgagctcta cgacacggtg tcgttcccgc
     6301 cgggcgtatg ccgcgcgttc cgcaatgtcg gcaacgagga aggcatcctg caggtcatca
     6361 tcaccggcgg cgtgcacgac atgaccgaca tcgacttctc ggaaaacgcc aagaaagaga
     6421 tcgaagccgt gcgcccggga ctgtccgcgg agttcgaaaa ggcgggcttt accttttcga
     6481 ttccgttccg aagctgagcc ggttgccgtt gagttgcgta atcgcatcgc cgatcgattt
     6541 cgcatcgcgc ggccgcacca ggcgcgcgag ctccttgccg tcgcgaagaa aaataagcgt
     6601 aggccagagc ttgatccgga acgagcggcc gaggcgcttg ccgctcgagt cggcgatctt
     6661 gaggtggcgg atgccggcat ggccggcgag cgcctcagca atcagcggct gcgcggcgcg
     6721 gcagtagccg caccacgggc tgccgaactc gatcagcgta gggcctgcga actggtcgac
     6781 ctcggcgcgc ctgggctccg cctgcgcgta cgtagtgttc atctccgcgg acgcccaaag
     6841 cgcttgcaga attcgccatt gcccttccag ctctggtcga gcaggcgggc gtcgcggaag
     6901 gtgagcgtgc ggtcgcagcg cgccgcggcg ccctccggtg cgccgaacac cacgccgccg
     6961 gcgccgcctc ccgagcgctg cgcttgcggc gcgcggtcct cggagagcca ggtgtgggaa
     7021 tccttcgcgc tctgcgcggg tgggccccac gctgcgacca cctgctcgta ggtcgcaccg
     7081 cgccaggaat cgcgcgccgc cgtgagctcg tggtttgcgg cgcaggcggc aacgccgccg
     7141 gcaaccagcg ccgcgagaaa tctcgtccta cttgctgcgc gcgtagaccg agcagatttc
     7201 atcggggccg atccagctct gctcgaccag tcggccgtcg cggaacgtca gcgtgcgctc
     7261 gcagcgcgcc ggcggtgcga ccgagccctc gccgaccgga acgctggcgc cgaccccgac
     7321 accgaccccg ccgccgcgcc cgccgccgcc gaacccgccg atgccgaaac cgaccgacgg
     7381 accgctgcgg taggtcggcc cggtctccga ccaccaggtg tgcacctcgg cgccgtcggc
     7441 gagcttcccg ctgcgcacag ggggccccca cgcgcgaact gcgtcgtcgt aggtggcgcc
     7501 ggtccagctc tccttggctt tctcggtcgt agcgcaggcc gccaggaacg ccaatgcgaa
     7561 gactgcttta cgtctgtcga tgttgtcctc cgcgctgcga taccccggcg gtggtttcgc
     7621 cggtggtctc gctgtcttca ttctgcgcga cgtcgccgag cgagacaatg ccgacgagcc
     7681 gcttgtcgcg gctcaccacc ggcagccgcc gcacctggat gtcgcccatg ttcctcgcca
     7741 cgtgctcgag atcctcgtct tcgtagcagt acttgacgtc cttgctcatt acttcgcgga
     7801 ccggcgtatc cggtccctta cccttcgcga tcgcgcggat cgcgatgtcg cggtcggtga
     7861 tcatgccgac caggcggtcg ttctcgccga ccggcatcac gccggcgtcg atctcggcca
     7921 tgattttcgc cacgtcgcgg atgctctgac cggggttggc gatgcgcacg tcgcgcgtca
     7981 ttgcttcgct tactcgcata ggacctcctg tggggttaga ccgctctttg acccagcaag
     8041 agatagaccg cgacggtgac cgcggcgacg gtcgccgcga tgcagcatcc gaccgccacc
     8101 gccgccatgg ggatggcgaa catcgcctgc tgcgccgcgg cggcctcggc gggcgagatc
     8161 aatagcgcga gcgacgcggc gatcacgatc cagcgccgga tgtagggcag cagcatccag
     8221 cgcatcgtgc ggttgtacga cagcgcgttc gcgcgcgcag gcgccggtcc ctgcgaggcg
     8281 tccttgaaca gcgcgaacgg atcgaggtag atgaggatct ggcgcacgag cggcaggcgc
     8341 gagcgcgcct cccgcttgag gagcatttcc acctggccgt cgacgacgcg cgccatgcaa
     8401 tcagtatgcc aaaaccaaaa aaaagcggag agctaatgct ccccgctttt ttgttacagc
     8461 gtgttacccg gtcctagtac ttcttctcgc cgggcttggt gtcgaccttc gccggcggcg
     8521 ccgggggctg ctgcggcttc tgctcggtag tcgaggcccc tgctgcgggc gtcgacgcgc
     8581 ccgccgctgc ggagccttcg gccttcttct cggctttcgc cgacgcgccg cccgcggccg
     8641 agctgatgcc gagcgcggca agcgtctgct ggtcgagctg gccgctcgcg ttgatgccct
     8701 tggaggtctg gaattccttc agggccttct gggtctcggg acccatcttg ccgtcggcct
     8761 ggatgttcag cttggtctgc acctgcttga tggtctcggc gtcgtggctg ccgtggctca
     8821 tggacgagcc gcccgccgcc gccgagcctt cggacttctt ctcggtggac gcggacgtac
     8881 tgccgctcgc cgccggcgag ctcgcgctcc ccgacgtgct ggcgctgccc gacgcgctac
     8941 cttgcgcttg ggtactcggc ttctgctcct ggctcatcga cggggtctgg cccgcctgcg
     9001 actcctgctt cttctcttgt gcgacggcgc cgaacgacag cattgccgcg gctaccgcaa
     9061 ctgcgatggg gctgaacttc attgaatctc ctttcctgga tataccgatc ggtggactac
     9121 cccgaattag cagcaagcgc cgtgccatcg aatattgacg ggaacatccc gacggccgag
     9181 cgactccaat gagggatggg cctagcgccg caaacgaaga tcctggtcgc gatggagaac
     9241 gaggaccgaa tggtgcgcgt tctcgacggc cattacctcg ccgtggtcaa gacgtgcgac
     9301 gaagcgcggc tgctgctcga gcgcgagacc ttcggcatcg tgatcctcgg cgtgcacttc
     9361 gacgaatcgc agatgttttc gctgctcggc gacatccgcg cacatgcgca ataccggaaa
     9421 gtgccgatcc tgtgcgtcct cggcgggcgc gggcgcatcc tcaccgatgt cgcggtcgaa
     9481 gggctcgacc atgccgtgaa ggcgatgacg gcgaacggct tcctcaacct cgagaacttt
     9541 ccagacgacg agaaggggaa cgcgcggctc cgccgcatcg tcgactacct cattctgatc
     9601 gacggcgacc tgcagcagct cccggagggc agcgagaacg gcaagccggt cccgggacac
     9661 ctgctgggcg agccgcggcg ccgggcgagc gaccctcaga cgcactagcc gccgggccta
     9721 ggccgggaga tcgatcaggg cgaggccgct caccggatcg tgcctgcgtc tcaggtgcgc
     9781 cggcgattcg gtcaggatca cttccagcgt cggccgcacg cgggccgcga gcagatgccc
     9841 gccgcacgcg ctgccgtcgc gccggccgag aacgacgtgc gcgtgcagct ttggcttacc
     9901 gtcctgcagg gcgatgtcgc ccaccagcgc cagcacctca acctgctcct tcaccggaat
     9961 gcgctcgtag tccttcttgt cccagtcgaa atagcccagg gtcgcttcgc tgaaagcgcc
    10021 gatggccgtg aacgcggacg cgcccagctt gcgctcttcg gcgaagcgcg tcaggccggc
    10081 caccggatcg tcgccggtct cgaagatgac cgcgaacacc cgctcgccgt tgtcgttcag
    10141 cagctttgtt cgcatgtcac tccgagctcg gcccgggttt ccttgtccat ctgcccgctg
    10201 gccggaatcg tcctggagag ctggaactgc gcgagcgcgg cctgcgtctt ctcgccgaaa
    10261 tcgccgttga ccgggccggc gtcgaagccg agctcgcgca gcctctcctg cacccggctg
    10321 atgaagccgg aatgaggtcc ggtggacacg ggttgctcag ggtccgggat ggcttcttcc
    10381 ggatgcagcg acgtgaacgg gctcgcggcg aagacgccga ggggcgcgag gatgaggcac
    10441 acagtcagca ggattcgctt catgaccggc atcagcaatt tacataccgc cgtacagcta
    10501 ccgtcgctgc tgatccggcg gcgtgtccac cgcgatcccg tgctcgaaga ccatctttcc
    10561 gccgagccaa cccgagatcg ccagcagccc gacggaaacc agggagagcc acaccagctt
    10621 gcccggattg cccggctctg acaggcgcat ccagaagttg atcgcgtaga gcgcgaccac
    10681 caccaggttg atcgccatgt gcttgagcgc ggtgcctctt ggtcccgcgg gcagcgacac
    10741 caggtcgatc agcccgggca ccgcggcgag cagcgcgccg atgatgccgc cgcccatgca
    10801 gtagaacgcg accgtcgtcc acgccgggtt ggcgctgccc cccgcatgaa tgagatcgca
    10861 taccagcgag aagatccaca gcccgatcgg gatcgggacg agcatcgggt ggatgggatg
    10921 gcgggcaatg ctggcgggag ttcgcatggg aaacctctga tgaaggaggg gaaataggaa
    10981 agtaggggaa ttcaatggga acgagtcttg cagcgattag atggcacgac ccggccggat
    11041 atccccaatc ggccggacat tccggaacca accgaggaga ttgatatgag acgcaacacg
    11101 ctgatcgcag tttccatcgc cgccgctttt gccttgccac tgaccgttca ggccgggggc
    11161 gacaaggcca gccagggcgc gagcagctcc gccgccaccg gcgcggagaa gatgtttcag
    11221 tcgctagaca agaacaagga cggctccctg tcgcgcgacg aagtgaaggg cagcccgcac
    11281 gacaaggact tcgcgacgct ggacaagaac aaggacggca agctcacgcg ccaggagcac
    11341 gcagcggcag ccgagcacgc gggcgacaaa gccgccgccg gcggcacgag cagcaagccg
    11401 aaatactaaa aagtccgaat gcccggaaag tctcccgcga cctcgtggcg cttgccaccg
    11461 tcgtcgtacc aatagagctt tccgggctct tccccccgcg tgagcttgtg cgagctgtcg
    11521 cagtaaggct ggttcttcga cagcccgcat ttgcagaacc actgctcctt gccttcgatc
    11581 acgaatttgg tgggctcaac gggcgtacgc ttgacgatcc gggccatcgt tattcctttg
    11641 ggtggcgagg ttaaggtgcc aacgtgccct gcgcgcgatt attcccgagg cgatgcggac
    11701 gaaacgaaag ctcctgtacg cctggctgct cgccacgctg ctgagcggca ttccttcgac
    11761 cctctacgcg ctgttcactg gaggtgatgc aaccgaagca acgcgcgctg caggagcaat
    11821 gcttctgccg tccgagacaa aactccccct attggtgaca gcggctgcgt tgatccacgc
    11881 atccgtgtct ctattctggg cggtggtgct gtggctcgcc ctgccccgcc gccgcacgat
    11941 gctgtgggcg ctgctcgctt ctgcggcgat cgcgctcatc gacctgcgtc tcatcgcgcc
    12001 ggcgtttttt cccgaggtgg cggcgctggc gttctggccg cagttcgccg accacctgat
    12061 gtggggcgcg tgctacagcg tcgggttgtc gctcggcagg ccgagggcga cgcggccgta
    12121 agtcgttccc gcgatgtcgg tgttaaagga gaaatgctgc ttcacggcgt gcgcatcgcg
    12181 gaacgcgcgc gccacggggc cgctctcgta aagcccgctc gctccataga gcccgaacat
    12241 gacgtcgacc gcgcgtaccg cccactcgac cgaataggcg ccctccaggc gaaagcgcgc
    12301 tttcgtctta aggtcgggca gcttttcttg cgcttcgcgg cagttcgcga gctgcaccag
    12361 gcgcgaggcg cgggcgtagg ccgtcgcttc gccaagccgg atctgcgtgc tctggatctc
    12421 cgccacgcgc gccccggtca tccggcctga cgcgggcttg tagtcgtcga tcaggccttc
    12481 ggcgatgccc agcgccacgc ccgagagcat gaaagggaac agcgcgaaca ccggcatccg
    12541 gtagagcggt ccgggattca ccgcgctgcc cggcgtgggc ccgcccttgg tgtcggcgac
    12601 cgcgagcgtg aggtgctcgg gcacgaagac ttcgcccgct tccacgtctt tgctgccggt
    12661 gccgcgcagg ccgccggcga accagttgtc gatcaccttg tactgcgagc ggtgcagaag
    12721 aaaaatgcgc ggctcgggcg gcaggttgtc gtcgaggcgc gcgaggccgg cgagcatgtt
    12781 ccactcgctc gggtcgaccc cgctcgagaa cggccagcgg ccggagacga cgtagccgcc
    12841 ttccaccttg cgcgccttgc ccgccggaaa caccgacgac gaggcgatca gggtgtcggg
    12901 cgatttcccc cagatctcgt cctgcgcctg cggcgggaac atgccgagca tcatgtggtg
    12961 gatggtgaag ttgacccagt tccacgaggt cgaggcgcag gcgcgcgcga gcagcgcgcc
    13021 gaacgtcacc acggtctcga actcgagctc ggcgccgccg acgcgcctgg gctgatgggc
    13081 gcggaagagc tgcgcgtcgt ggaagtcgcg cagcgtgtcg tcgggcaggc ggcgcagctc
    13141 ctcgcagcgc gcggcgcgct ccttgagctt cggcagcagc gcttcagcgc ttgcccagag
    13201 cttctcgtga ataccagtca aggatctgct ctccagtcca gatcagcgcg cccgggcgct
    13261 gcaggatgta ttcgtagagc ctctcgagat acgcgatgcg gtgcggcacg ccgctgatgt
    13321 acgggtgcac gctgatcgcc atcacgcgcg ccgatttctc gccttcggca tacaggcggt
    13381 cgaactggtc gacgccgcgc cgcagccatt cctccgacgg atggttctgc agcaccatca
    13441 tcgcgatgtc gttcatctcc acggtgtagg gcaccgagac gatcgggccg tgcgcggtct
    13501 ggatcgtgca gggctggtcg tcgagcaccc agtcggcgac gtactcgatg cccgcctctg
    13561 ccagccagtc gatcgtgtcg taggtctcgg tgagacccgg gctctcccag cctctgggcg
    13621 gctttcccgt gaactcgcga atcgcctgca cggtgtctgc gatggcctct cgctggtcct
    13681 cgacgtggtg catcggccgc tggatgtagc cgtgccccat gaattcccac ccggccttca
    13741 gcgcggcctc cgagacgcgg ggatagcttt tgacgaccga gccgttgatc gcgagggtgg
    13801 ccttgatgga atatttctgc agagcttcga gaaagcgcca gaaccccacc cgcatgccgt
    13861 actcgtgcca cgaccagttg ggcaggtcgg gcaacagggg ttggccatac ggcggaggca
    13921 gcacggtgcg cggcatggcg cgctcgatcg accattcctc gacgttgacg atggtccaga
    13981 ccgcgacccg cgcaccgttg ggcagcttga gcgctggacg atcgacgatc gccgagtacg
    14041 cgatgcgatc cttgggcagt ttcatcctca gttaatatac cgattaagct ccttggagga
    14101 ggaccgcatg ttgaacggca tgagattgct gtgcgtagcc gccgctgctt tcgccacagg
    14161 cacacaagca cagacggtgg gcttcgccac gctgccgccg ggctcgatcc tgcacgcgca
    14221 ggcatccgtg atcgcgaagg tggtgcagga caactccaag ctgcaggtgc gcgtcgtcgg
    14281 ttacggcggc gacaccggga tcctcgaagc ggtcaactcc aagcagtcgg acttctggat
    14341 cctcgacgtg ggcgagagcg cggacgcgct gcacggccgc ggcacatgga agggcaatcc
    14401 gaagccggcc ctgcgcaccg cgctcacgat gttccccttc cagatggcgt tctgggtgag
    14461 gaaggactcg agcatccagt cgatcgccga tctcaagggc aagcgcgtgc ctgccgaatg
    14521 ggtgcagcag accggcgtga tcccgcatac caccgcggtg ctcgccgccg gcggcatcac
    14581 ctacaacgac gtggtgaagg tgccggaggt gaacgtggtg cgcgccgcgg acgacttcaa
    14641 ggcgggcaag ctcgatctcc tgttcttcgc ggtcggcgcg cccaaggtcg ccgaggtgtc
    14701 cgcggcggtc ggcggcctgc gcctgctacc gatgaatccg ctgccagatt ccgagaagcg
    14761 catgaaggcg gtgcggccgg agtactactt caccaccgtg aaccctgcgc cccacatcgc
    14821 cggcgtcgac aagccgtcgc cggtgcagac aatcgacgtg gtcgttgggg tcggcgcgca
    14881 tgtttccgac gaggtcgtgt acaacttcgt caaggcggtg cacggcaaca aggccggcat
    14941 ggtcgccggc catccgaact tcaacgcgtt ccaggagaag gcagccggca agccgcagcc
    15001 gagcctgccg caccatcccg ccgccatcaa attcttcaag gaagcgggta tctggcgcgg
    15061 atagcgcaag gcgcgctggc cgcgcttctc attgcaggat cgatcgcctg gtcgctggac
    15121 ctgttccggc cgatcgggtt ggtgctgttc tccgagcagt tcgccgccgc cgcgtacggc
    15181 atcggccttt cgctgacttt tctccggttc aggttttcag ggaagagctc tgacgtcccg
    15241 tggtacgact ggctgctggc ggctgcatcg ttcgccgcgt gcggctatac cgcggtcaag
    15301 ttcccgaagc tgaccgcgct cgccagcacc accacgcccg aggtgctgat cctgtcggtg
    15361 ctgctcacgg cgctggcgat cgaggcggtg cgtcgcacca tcggctggtc gctggtgatc
    15421 atcctgctcg cgctcgccgg ctacgtgctg atcgggcacc tggtgcccgg ccagctgcag
    15481 acgcgcgagg tcggggtcgg ccagatgctc gtgtacctga acatggacaa caacggcctg
    15541 ctcggcctgg tcctgcagat cacggtgatc gtgatcattc cgttcgtgct gatgggccag
    15601 ctcctgatgc gctcgggcgg ctcgagcttc ttcaacgacg cggcgatcgc gctcatgggc
    15661 cgctaccgcg gcggcgccgc gaagatggcg gtggtcggct cgagcctgtt cggcatggtc
    15721 tccggcatcg ccgcggcgaa tactgtggcg gtgggcgtgg tcacgatccc gctcatgaag
    15781 cgctcgggga tgtcggcgaa gctcgccggc gcggtggaag cctgcgcctc caacggcggc
    15841 cagctcatgc cgccggtgat gggcgcggtg gccttcgtga tggccgactt cctgcagatt
    15901 ccgtacacgg aagtggcgat cgccgcgatc ctgccctcgg tgatgtacta cgcggcgctt
    15961 ttcatccagg ccgacctcga ggccgcacgc tacggcttcg gcaaggtcga ggaagacaag
    16021 atcccgcgca tcgggcgcgt gctcgcgcag ggctggctgt tcctcgcgcc gttcgccgtg
    16081 ctggtctata cgatgttctg gctgaatctc gagcccgagt acgccgcgat ggcggcgagc
    16141 gcgaccatca tcgttctcgg cttcgccttc ggctatgccg ggagccgcat gcgccttgcc
    16201 gacatctggc atgcgatccg ggagaccggc acgagcctgt gcgagatcct ggtgatctcg
    16261 gcagcggccg ggttcatcat gggactcttc caggtgagcg ggcttgcctt cgccttcgcg
    16321 gcctacctcg tgcagctcgg ctcggaaagt ctcgtgcttc tactagttct ggcagcggta
    16381 gtgagcatca ttctgggcat ggggctgccg acgatcggcg tctacgtcat gctcgcgatc
    16441 ctggttgcgc cggcgctggt caaggtcggc gtgccgccgc tcgcggcgca cctcttcatc
    16501 ctgtacttcg gcatgatgtc gctcatcacc ccgccggtcg cgcccgccgc ctacgtcgcg
    16561 gccgcgatcg cgggcgcgcc ctcgatggcg acgggctgga ctgcgatgcg cttcggctgg
    16621 agctcgtaca tcgttccgtt ccttttcgtc tattcccccg cgatcctgat gcagggcagc
    16681 atcgtcgaca tcgtcaccgt gactatcacg tcgctcttcg gcatctggct gatctgcgcc
    16741 gccatgacgg gctatttcgt gaaaacaatg aattggaaaa cccgatgcgc tttcgcgctc
    16801 gccgggatca tgatgctctc gccgcaccag gtgtcggagc tcatgctctg gattaacatc
    16861 ctcggagcgg cagccgggat cgcgctgatc gcttacgagc tcagggcaag gagggcttat
    16921 gtacgtgcgt aacgcctggt atgtcgcggc ctgggaccat gaggtgacgc gggagatgcg
    16981 ccgccgcatc ctcctcgacg agccggtgct cctcttccgc aaggatgacg gcacgccggt
    17041 ggcgctggag gaccgctgct gccaccgcca ggcgccgctc tcgatgggca agctcgtcgg
    17101 caacatcgtg caatgcccct atcacgggct gcagttcgac acgacgggca agtgcgtcaa
    17161 ggttccgagc caagacatgg ttcccgcctc ggcccgggtc cgctcctacc cgatcgtcga
    17221 gaaaaaccac tggctctgga tctggatggg cgatcccgcc aaggcgaacc cggagctgat
    17281 cgaagatttc cactggctgg acgaccccgg ctggcgcttc ggcggcagct accttcacgt
    17341 cgatgcccac taccttctgg tcgtcgagaa ccttctcgac accacccacc tccccttcct
    17401 gcaccccaac acgcttggca ccgatacctt cgcgcgctcg gagttcgacg tgaagcgcga
    17461 aggcgaccgc atcatagtgt cgcgctgggt gatgaacgag ctgccggcgc cgttccacaa
    17521 gcagatgggc ggctttcccg acggcctcaa ggtcgatcgc tggcagttca cgcatttcgg
    17581 gccgccgtgc ttcgtgaagc tcgacgtcgg ctcctcgcct gccggcgccg gcgcgcgcca
    17641 gggcgaccgc agcaagggcg tgaacatgtg gaacctgaac gcgatcacgc ccgagaccga
    17701 aaagagcgcg cactacttct tcgcgcaggc ctacaacttc aagctcaacg agcagtgggt
    17761 ctccgacatg ctccgcaccc aggtccacag catctttctc gaggacatgg cgatcatcaa
    17821 ggcgcagcag cagaacatgg acctcggcac gagcccggtg acgagcctcg gacaggacaa
    17881 ggcctgggtc gcgatgcgcg gcatcgtgca gcgcctgatc aacgaggagc aacaggcgac
    17941 gcgtccggtc gccgtctctg cctaaggcgc gaacggctct ttttcttatc tcctcaaacc
    18001 gagggattga tgagcgccag tcccggcact gctgcgaaat gcgcatcgaa gctcagcagc
    18061 ggcattcgat gctgccgcac cagcgccgcg atccacagat cgttcgtcgg gatcggtgtc
    18121 ccttgcctgc gcaaggtcac gaacacatcg ccgtaatgct gcgccgtctt ttcgtctggc
    18181 ttcaggacct gaacgcgcgt tgacgccatg aatcgcgtca gctcttcacg gttcttctgc
    18241 gctcgggtcc cggcggcgaa gcccgcaagc aattcgccca gcacgatcag cggcaggtag
    18301 atctcgcctg cagcgcgcag gaattgcacg gtcttcggtt caccgcccag caatccggcg
    18361 taggcattcg tatcgatgat cagcgcgctc acttgcgcgc cttgcctccg gcggcgggcc
    18421 ttgcgcgata cgcggctttc ggctcaccgg cccacagccc ggtttcgatt tctgcgaacg
    18481 gcgcgacggc cgcgaggaac gcggcagctt cggttttcga ccacgcgccc gccagcgcgt
    18541 ccaggtcgtc ggacgccggc tcgcccgcag cgtattctcg ctgcagcgtc tcgacgatca
    18601 ggcggttgac gctgatattg cggcggcgcg ccctcgactt gatgcgcgac agggtcgaag
    18661 cgtcaaggcc gcgtaggctg atgttgggca tggaggagcg tcccgtgata gcagttgaca
    18721 gcatgatatc atttggggtt ccacctcggt tcaatccagc ttgacccgat cgacgtagca
    18781 ccacttccag ctctcgcctg gctcgatcga gcgcatgacc gggtgaccgg tggcgtgaaa
    18841 atgcgcggtg gcgtgcctgt tcttcgagct gtcgcagcag ccgacgttgc cgcacttcag
    18901 gcacatgcgc aggttcaccc agcgctcgcc catgcgcagg cattcttcgc agccgttgct
    18961 gctcggcgcc actcccgcga tctggtcacg gtgagtgcag gctttggctg ctgccggaaa
    19021 aagcagcgac gcggcgatcg agccgaccag gatcgcgacg atcgcggcga gcgaagcgag
    19081 cggcgggacg tgatacaggt cgcccacggc aagcttggcg gcggccatga aaagcatcac
    19141 cgcgaggccg accttcagga agcgcagctt gcggatgacg ccggcgagca ggaagtagag
    19201 cgagcgcagg ccgagcatgg cgaacacgtt cgaggtgtag accaggaacg ggtcgcgcgt
    19261 cacggcgaac accgcgggga tcgagtcgac ggcgaacagc aggtcggtgg tctcgatcac
    19321 ggtcagcgcg accaggagcg gcgtcgccat caggcgtcca tccaggcgca cgacgaagcg
    19381 cgagccgtgc agcctcggct cgatcgggat gaagcgcgcg agccagctct cgcacagcgc
    19441 gcattgccgc tcggcgaagc gttcctgccg ctcctcgccg cgcagcatgc gataggccgc
    19501 ataggcgagg agcgcggcga gcgggtagac cgcccattgg aagcgctcga ggatctcgac
    19561 cccgacggcg atcagcgccg cgcgcatcgc cagcgcgccg acgatccccc agaaaagcgc
    19621 gcggtgctgc aggctcgccg ggatgccggt gagcgagaag atcaggatga agacgaagag
    19681 gttgtcgacc gagagcgatt tctcgaggac gtaagcggtc aggtaggtga ttcccgcctc
    19741 cgtgccgagg cgcagcgcaa tccacccccc gaagccaagg ccgaggccga tccagaccgc
    19801 gctccacgcc gccgcgctct tcgggtcgct ctttttcgaa aacctgagat cgaccgcgag
    19861 cgcgatcgcc accacccccc agaaggcggc ccactccgtg gtcacagccc ggcgtcggcc
    19921 gcgagcttgc cggcgagcgc ggcccagtcc tggtgcgccc ggccggcggc gatcgcctcg
    19981 agaaggtgcc ggcggatcat ttcgccgctg ggcagcggca tgccgacgtc ggttgcggtg
    20041 gcgagggcga gctcgacgtc cttgaccccg agcggcatgg cgaactgggc cggcgtccac
    20101 gcctcctcga cgatcatctt tccgtagttc ctgtaggccg gggaacccag ggaggtactg
    20161 ctcagcacct ccaggaacct ttgcggctcg atcccgcact tgcgcaccag cgcgaacgct
    20221 tcgccgaggc tctcgatcac cgtggcgagg aggaagttgc gcgcgatttt tactgcgttc
    20281 gccttggcgg gttcctcgcc cacgacgaag gtctgttttc ccaggatctt gaacagcggc
    20341 tcgcagcgct cgagcgccgc cttcggcccg gcggcgatga tgtcgagctg gccctgcgcc
    20401 gcgagcggcg ggcggccgaa gaccggtgcc gcgacgtaca agctcttctg gtggaccgct
    20461 gtcagcttgc gcgcgatgtc catggagacg gtcgacatgt tgaggtggat cgcgtcccgg
    20521 ggcaggcgcg aggcgagccc ggactccagc cagaccgcgc gcaccgcggc gtcgtcggcg
    20581 agcatggtga tcacgatctc cgcatcgaga accgcgtccg ggtgctcgag gacttccgcc
    20641 gagtggcggg tgcggttgta gactttcagg ctatgccccg cacccctcag ccgctcgacc
    20701 atcggacgac ccatctggcc aaggccaaca aaaccaacgt tcatctcggc ctcctcatag
    20761 cagtctgttt ctttattggc agtgccagcg cgcaatccta tcccgcaaag ccggtgcgcg
    20821 tggtcgtgcc gtggccgccg gcgggcgtca ccgacgtgct gacgcgcgcg ctggttcagg
    20881 ccatgagcga atcgaccggg cagcagttcg tggtggagaa ccgtccgggc gcgggcggca
    20941 cgctcggcct cgcgccggtg gcgaagagcg cgcccgacgg ctacacgctg gtggcgagcg
    21001 acgtgccgag ccatgcgatc agcgcgacgc tgtatgcaaa gctgccctac gacgtgctcc
    21061 acgacttcga gccgatcgcg atgatcgcgg gctcgccgat ggtgctggcg acgaaccccg
    21121 cgctcggggt ccgcaatctc aaggagttca tcctcgttgc gcgcgcgaag tccccgccga
    21181 tgtcctacgg ctcgtcgggc aacggctcga tcacccatct cgccatcgag cgcctgaagc
    21241 gcgacgccgg catggacttg gtgcacgttc cctacaaggg caccgtgccc gcgatcacct
    21301 cggtgctgtc aggcgacacg gcgctcgcct tcggcacgat cccgggcgtg atgccgcatg
    21361 ccaaggcggg caagctcgtg ccgctgggct cctccttcgg caagcgcttc tcgcagatcc
    21421 aggaagtgcc ggcgatcgcg gaaacgattc ccggctacga catgggcttc tacaccgccc
    21481 tcttcgcgcc ggcgaaaacg ccaccggaag tcattcaaaa agttttcctt gaagtgacga
    21541 aggccttggc ccagcccaaa gtaaaggaga tcttcgccaa cagcgcggcc gagccaggca
    21601 cgatgacgtc cgcagagttg aggcagtacc tggcagccga ggtaaaagcc tggggcgacg
    21661 tagtccgttc ggtgggcctc aaagtcgact aaggggcatg aagcttgcga gcggtgccgg
    21721 gaaggagacg ttacgtgacc cagcccaagc gaaagaagca acggcacaag cagccgccga
    21781 ccggatcgac caccaacgcc agcgacgccg tcgacgacat cgagctgccg ccgaatccct
    21841 cggccgagga gaaactcgac gccggcgtgc aggaaacctt cccggcgagc gacccggtca
    21901 gcatcgcgca accctccgaa accgcctacg agcgggagaa gcgcctcaag cgcagccgct
    21961 agcgttttcc tccgaggagc cgggccgcca tgagaagctg caggagcagc gagaacgcga
    22021 gggcggttga atagccctgc gggtcccagc cgcccgaggc ggtgcgcggc cagaaatcga
    22081 gcacccagcc cgtcgcactt tgcagaacga acgcgcctgc cagcgtgagg gtgttgaccg
    22141 cagtcgctac gcgcgcggtt tgctcgggcg ggaacatctg gccgacggtg atgtagccgg
    22201 cggtggcgcc ggcgacgcag aaggcgaaca gcatccacag ccccccgacg acgagcggcg
    22261 cggcgggctg cagcaccagc ccgatctgcg cagccgcctg tccggcggtg caggctagcg
    22321 tgaccagcat cggcggatag ccgcgcgcct tcgcgcgact cgtcgccgcg ccgacgacaa
    22381 gcgcgccgac catcatggtc accgtgtagg cgaggagcgt gctcgcgctt tgccggctgt
    22441 cgtagcccgc cacgtcgcgc agccacggcc cggcccagag accgagatag gcgaagttga
    22501 ggaccgagag catcgccacc gcgggcgcaa agcgccagaa gacccgcgag ccgaggatcg
    22561 aggccatgat gccgagctcg gttttcagcc cgcccgagta gctgccgcgc cgatccggca
    22621 ccgaggcgaa aatccacaac gccgtcgccg cggagaggcc gcaaagcacc cacaggacgc
    22681 cgcgccagcc gagcgctggc agcgcggccg cgaccggcac ggtggacgcc acgctgccca
    22741 gcccgccaat ggcgactgcg atgcccgtga ccaaggcgac cttcgccggc acgaaccatt
    22801 gcgcgttggc cttgatgatg gccatcagtc cggcggagat gccgacgccg acgatcacgc
    22861 gcgcgatcac cagggtcgcg aagctgtcgg cgagcgcgaa gagcgcgaat cccgcggccg
    22921 tgaccagggc caacgcggcc tgcacgttgc gcgcgcccac ccggtcgagc gccatgccgc
    22981 ccggcagctg cacggccgcg taggcggcga gcatgcaggc ggcgagcagc ccgagctcac
    23041 tcgccgaaag cgcgaactcc accgccagca ccggcccgat gaccgccatc accgtgcgcg
    23101 ccgcctggtt gatgagcccg aacaccgcaa gcggcagcgt gacgcgggtg aacaggcctc
    23161 tagaatgcga atcagcaccc atggagcgca tcgtcgtgca ccctcctcgc ccaggcctcg
    23221 acatggctgc gggctgcggc tgggtgcaga gcacggcgat ccgcgccggc gacttcgttt
    23281 tcgtctccgg catcgtggcg ctcgatccgg agagcggcga gcggctgaac ggcacgctga
    23341 ccagcgaagc gcaccgcgtt ttcctgaacc tcaagctggt gctcgaagcg gcgggttcct
    23401 cgctcgagcg gctggtccag gtgcgggcga tgatctacga ccgcatcgag tacgacgcgc
    23461 tgaaccgcgt ctaccgccag tacgtgccgc acgggccgcc ggcgcgcacc gtctggagcg
    23521 tacgcatcat cgacgcgttc aaggtccagc tcgacgctgt cgccgtggtc tgatcgagga
    23581 gcaagcccat gtccgtcgcc cgcgaaatca tcttgccgaa gaacgagcgg ctcaacgtct
    23641 cgcgcaactt caacctgccc cacagcccgg gcgtgcgcgc cggcgacttc atcttcctct
    23701 ccggcatgct ctcgaccgac ccgcagaccg gcgagcgcag cccgggcacg atggcgcacg
    23761 agacgcgaca aacgctcacc aacatgcgcc acctgctcga atcggccggc tcttcgctcg
    23821 agcgcgtggt gaagatcaac gtgctgctct acgacatgct cgagttcgag aacatgaacg
    23881 cggtctacag gcagttcttc cccgtcgatc ccccggcgcg caccacctgc ggcgtacagc
    23941 tctccgcggg gatgaagatc gagatcgagt gcatcgcgct cgccaactaa accgtcgccc
    24001 ccgcgaaggc gggggcccaa gttttttttg agaaacatgg gttcccgcct tcgcgggaac
    24061 gacaagccgc cgctaggcgg cttcatggct gcgctcgatc ttcctcttaa tgtgctcgcc
    24121 cgcgctgatc gtcgcgtagc gcggcttctc gcccggcgcg agacaggtcg gcacgcattc
    24181 gatgcgctgg tcgtagctcg ggtgcacgaa catgccgatc gagaggcgcc ggctctgcgc
    24241 actgccgagc ggcgggttcg ccacccggtg cagcgtggaa gcccaactgc cgttggtcca
    24301 gcgcgccatc atgtcgccaa ggttgacgac gaactctcct acgcgcggcc gcacgccgga
    24361 ccaggagccg tcgggcaggc gcacctcgag cccgccttcc gcgtcggtgg cggcgaggat
    24421 cgtcatcgcg ccgtagtcgg tatgcgcgcc ggtgcgcagc tggcccggca agggtggctt
    24481 ctcgagcacc ggatagtgat ggcaggacat gatgctgaag tgccggtcga gcgtgccgtc
    24541 gaagaatccc ccgggaagac gcagagcggc agcgaagacg cgcatcaggt cgcgcgccag
    24601 gcgctcgtat tcgcggtaga gcgccaccag cgtaccggcg aggccggcgg gctcgtcggg
    24661 gatcaggttc ggcgcatagc tcgtcgccgc ctccggcatg ccggcgtaat aggcgcgatg
    24721 gtcgtcgacc gggccgagga agacggtctc gcgcaggtcg ggtggcgtct tctgcccgag
    24781 ggtgtacgcg aggcctcgcg tggcaaagcc gtggtagccg cgctgccgcg acggtccgct
    24841 cggatgccag cggttcttgg tcgcctccgg caggtcgaag aaggcgaaga gctcgcgctg
    24901 cgcgcgctcg agcagcgtga gcgggaagcc gtgaccggaa atgatcagga agccggtctg
    24961 ctcgcaggtt cgcgccaccg cctctgcaat gccgggtctc gagatgtcga tggtcggaac
    25021 ggtgttcgtc attttcgtga aagctcagcg tccacgattc tctcgtaagg cgggtcggag
    25081 cggatgaggc cgcccgagac cagcgcagcc ttgagccgca ggaacggcgc cggcggcaga
    25141 tcgggcgtcg ccgcccacag gccgctcgcg cgataggcgc cgatgatgcg cgcgagcgcc
    25201 tcgcgcgaga gcttcggcag gaagcggctg accgcgtccg ccagcgcgcc gggcgtggcg
    25261 gcgaagagcg cggcctgcgc gcgcgccatg ccgcgcacca accgccggca ggcttcgcgc
    25321 cgcgcgcggg tgacgcgccg cgtcgcgtag aaggtcgtat agacaatgtc gccgcgctcg
    25381 ctgaagcggt gccacacgtg accccggcct tccctgacca ggcgatcggc atacggctcg
    25441 aatacctgga cgacgtccac cgtgccgcgg cggaacgcct cgaggttgcg cgccatcggc
    25501 cgcacgcggc gcccggcgat cgccgccggg tcgaggcccg cgcgctgcag gtcgtcctgg
    25561 aaagtcatcc acggtgtcgg cacgtcgccg gccggcgcaa cgcgcaggcc gcagaggtcg
    25621 cgccagcgga accccggctt ctgcgtacga ccgacgagca ggaacggatc gcgcgccacg
    25681 acctgcccga agcagacgag cgggcagcgc gggtcggcgt cgtgatgcat catgacgcgc
    25741 atcggcccgc cccacgaaac gtccaccgtt ccgtcgagca gcgcggtcgc agttcgcgag
    25801 gtgctcggcg agacgacgtg gcggatgtcg agcccttcgc tttcccagta gccgccggca
    25861 agcgcgagat aaaagggcac gtaaggcacc gcgcgcatgt tctcgcacag cgtgatacgc
    25921 attaaacgtc tttcaggtat ttcgtctcca gatcccgcca gcggcgcgag ttcaccgctt
    25981 cttcccggtc gttgaaggtc g
//
LOCUS       DATBSZ010000160         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1732695, whole genome shotgun sequence.
ACCESSION   DATBSZ010000160 DATBSZ010000000
VERSION     DATBSZ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732695"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..497
                     /gene="mutT"
                     /locus_tag="VIG70_10625"
     CDS             81..497
                     /gene="mutT"
                     /locus_tag="VIG70_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEY8251052.1"
                     /translation="MREGSKRPIVIVAACALLDEKGAVLIAKRPPGRPLAGLWEFPGG
                     KIEPGEEPEEALIRELHEELGIEIEKRALTPLTFASYAYPDFHLLMPVYLCRRWRGEV
                     AAHEGQELLWVKPDTLHLYDMPPADEPLMIAFRDLL"
     gene            603..713
                     /locus_tag="VIG70_10630"
     CDS             603..713
                     /locus_tag="VIG70_10630"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251053.1"
                     /translation="MEYGILTLIAIAIMAVVSQIGGSVGGMFDMLKDAFN"
     gene            complement(710..1633)
                     /locus_tag="VIG70_10635"
     CDS             complement(710..1633)
                     /locus_tag="VIG70_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8251054.1"
                     /translation="MSDERLLFDRKLLRARRARFAPEIETHEFLLANVAREIAERVAL
                     MLRPFPLALDLGAYHGLLGRSVAELPSVGAIIYAESVFEFATLCPRPSLVCDEDLLPF
                     KAQSFNLIVSGLALHRVNDVPGALIQIRRALHPDGLFMAALLGARALSELRQALLEAE
                     AEIDGGASPRVAPFGDVREYGALLQRAGFALPVADAETLTVLYASPRELMREVRALGG
                     GNVLLARSRAPLSRRTLERAEAIYRERHASPDGRVSASFEIVYLSGWGPDASQQQPLK
                     PGSAAQRLADALGTTEQSAGDKAAFPAKPKS"
     gene            1691..2494
                     /locus_tag="VIG70_10640"
     CDS             1691..2494
                     /locus_tag="VIG70_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HEY8251055.1"
                     /translation="MPTGEAKPGLEAPAPARARGPGIVRPTLGRLADLLLPPVCIACR
                     ARIGSHGLLCGTCFASIAPPIIAPPICARLGVPLYYDAGEGALSAGPIATSRVYDRAR
                     AAARYSETVRELIQSFKYRDRQEGLALFARWLRQAGAELLGCCRSHRAGPALSRPAVV
                     AALQPVGHAGASVGLTGVEVDCFVLRRVKQTVSQVGLSADQRRRRRAFKVDRARAGRI
                     RGKNVVVIDDVITTGATAEACARALKRARAARVDVLALARVVEPTAFLL"
BASE COUNT          402 a          854 c          854 g          432 t
ORIGIN      
        1 agactatatc gccatcaatg ccgactaccg gtcctgagtc gggtatttag acaaaactgc
       61 attaacctcc ggatgccagt atgcgcgagg gctcgaagcg cccgatcgtt atcgtcgcgg
      121 cctgcgcact gctcgatgag aagggcgccg tgctgatcgc caaacgcccg ccagggcggc
      181 cgctcgccgg cctgtgggag tttcccggag gcaagatcga gccgggagaa gagccggagg
      241 aagccttgat ccgcgagctg cacgaggagc ttggcatcga gatcgagaag cgcgccctga
      301 cgccgctcac cttcgcgagc tacgcttatc ccgattttca cctgctgatg ccggtttatc
      361 tctgccgccg ctggcgggga gaggtggccg cacatgaagg ccaagagctt ctctgggtga
      421 agcccgacac actgcacctc tacgacatgc cgccggccga cgagccgctc atgatcgcgt
      481 ttcgcgatct gttgtgagtt tggggagtac catgggccat acaccttcgg aaccgacccg
      541 gccggcgata cgcctttcgg ctgccgtcca gcgcttcgcc gccgacggct ctggtcacgg
      601 ccatcgaata cgggatcttg accctgatcg ccatcgccat catggcggtg gtcagccaga
      661 tcggcggctc ggtcggcggc atgttcgaca tgctgaagga cgccttcaac taagactttg
      721 gcttggcggg aaatgccgcc ttgtcgcccg cggactgctc ggtcgtgccg agtgcatcgg
      781 cgagacgctg cgccgcgctt cctggcttga gcggctgctg ctggctcgca tcgggtcccc
      841 aaccgctgag atagacgatc tcgaaggagg cgctcacccg cccgtcgggg ctcgcgtggc
      901 gctcgcgata gatcgcttcg gcccgctcca gcgtcctgcg cgagagcggc gcccggctcc
      961 gcgccagcag cacgttgccg ccgcccagcg cgcgcacctc gcgcattaat tcgcgtgggc
     1021 tggcatagag caccgtcaag gtctcggcat cggcgaccgg gagcgcgaag cccgcgcgtt
     1081 gcagcagcgc cccgtactcg cgcacatcgc cgaacggcgc gacgcgtggg cttgccccgc
     1141 catcgatctc ggcctcggcc tcgagcagag cctgacgcag ctcggacaaa gcgcgggcgc
     1201 ccagcagcgc cgccatgaac aacccgtcgg gatgtagcgc gcgcctgatc tggatgagcg
     1261 cgccgggaac atcgttgacc cggtgcagcg ccaaaccgga gacgatcaga ttgaagctct
     1321 gcgccttgaa cggcagcagg tcctcgtcgc agacgagcga tgggcgcggg cacagcgtgg
     1381 caaattcgaa cacgctctcg gcatagatta tcgcgccgac cgatggcagc tccgccacgc
     1441 tccggccgag cagcccatga taggcgccaa ggtcgagcgc gagcgggaag gggcgcagca
     1501 tcagcgcgac ccgctcggcg atctcgcggg cgacattggc gagcaagaat tcgtgcgttt
     1561 cgatctccgg cgcaaagcgc gcgcgccggg cacggagcag cttgcggtcg aacaataggc
     1621 gctcgtcgct catcggcggg tccgtaccgg cacttttcgg gtgggggtca tgcgtcctat
     1681 catagcggtt atgccgaccg gcgaagccaa accggggctt gaggcaccag cccctgcgcg
     1741 cgcgaggggc cccggcatcg tgcggcccac gctcggccgg ctcgccgatc tgcttttgcc
     1801 gccggtctgc attgcctgcc gcgcgcgtat cggcagccac gggctgttgt gcgggacctg
     1861 cttcgccagc atcgcgcctc ccattatcgc gcctcccatt tgcgcgcggc tcggcgttcc
     1921 gttgtactat gacgcgggcg agggcgcgct ttcggcgggc ccgatcgcca cgtcgcgagt
     1981 ctacgaccgc gcgcgggcag cggcgcgcta ttcggagacc gtgcgcgagc ttatccaaag
     2041 cttcaaatat cgcgaccggc aggagggcct ggccttgttc gcccgctggc tccggcaggc
     2101 cggcgccgag ttgcttggct gttgccgatc tcatcgtgcc ggtcccgctc tatcccgccc
     2161 ggctgtggtg gcggcgcttc aaccagtcgg ccatgctggc gcaagcgttg ggctcaccgg
     2221 cgtcgaggtc gattgcttcg tgctgaggcg ggtgaagcaa acggtaagcc aggtcggcct
     2281 cagcgccgac cagcggcgcc gccgccgggc gttcaaggtc gacagggcgc gcgccggtcg
     2341 aattaggggt aagaacgtcg tggtgatcga cgacgtgatc accaccggag cgaccgccga
     2401 ggcctgcgcc cgggcgctga agcgggcgcg cgctgcccgc gtcgacgtgt tggcgctggc
     2461 gcgtgtcgtc gagcccacgg cgttcctgct ctagccgtca tttttacctg agcacgcgaa
     2521 cagtatccgt ccccttaatg gg
//
LOCUS       DATBSZ010000161        13164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_25423, whole genome shotgun sequence.
ACCESSION   DATBSZ010000161 DATBSZ010000000
VERSION     DATBSZ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13164
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25423"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1992
                     /locus_tag="VIG70_10645"
     CDS             <1..1992
                     /locus_tag="VIG70_10645"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8251056.1"
                     /translation="PRERVLVVLGFPDIGAAGDAVPQILAAGPIACEGLDEAIIGGLR
                     SRNLRLDDIALLPPGKAWLMVEFGADSPAEAVARAKSIGGMLITDRVLMSKLWTIRET
                     GASATALNLGGQGVDPVVGWEDAAVDPMRLGDYLREFQALVERFGYRTSLYGHFGDGC
                     IHARINFELRTPAGLAHWRRFLTEAALLVVKYGGSLSGEHGDGQAKGELLPLMFGPEL
                     MQAFREFKRAWDPHNRMNPGKLIDARPFDADLRLGPEYRPVSLKTKFSFRSEVGDGFV
                     RATEHCIGMGKCRSREGGAMCPSYRGTHEERYSTRGRARLLAEMLRGEVITEGWASTE
                     VQEALDWCLACKSCRSECPTHTDMAAYKAEFLSHYYETHRRPRQAWSMGRIGEWAPMA
                     SRFSGVANLLSSLAVSKRVAGVDLTRKLPKFAPRTFRSQFKPNGSGDPVVLFDDTFNN
                     HFRPGTAAAAQKLLARAGCAVELPARHVCCGRPYYDYGMVDEAKRALERVLEILPSDR
                     PIVVLEPGCLSVFRDELRQLLPDDPRAARLASNVFSLGEMLQKRNFKGTSHGRVFMHA
                     HCHQKALWGTAADLAILKSSGCEVLAPDTGCCGMAGSFGYRPQHAETSRRIAGLALLP
                     ALDASRDAVVVANGFSCREQIEALSGRATLHLAELLAPR"
     gene            1989..3215
                     /locus_tag="VIG70_10650"
     CDS             1989..3215
                     /locus_tag="VIG70_10650"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEY8251057.1"
                     /translation="MSLEVLLYLAAVCALSGFVHGAIGFGFPLVATPLVALVIDMRSA
                     ITLLAPVTVVLVVISALRGGGLGGLLRDFWFLPVGIAAGAWLGTRILLAVPPEPFTLV
                     LALMLIFYLNVDRLGRGHSETVVRLRAVFGIAFAFVAGVFEAVANVAGPVLLVYFMLL
                     GAAPQQIVQTLNLCFSVGKGSQVATLAASGALSAATWAAVAGLSVPAVAALAVGMRLR
                     DRIDAQAYRRWLRKALWVMAILLLAQFAHSALASEPLFKAIEEYREGAAVELVERGGV
                     DLNARNGSGDTLVHRAVETGMRQLLQALLAAGADSRARAKDGETALHLAALHPEPVFT
                     DLLLAAGADPRARNTDGESPLHWAALSGHIVVVQRLVAHGADARIRNGKGLTARDYAL
                     SQEHSEIAKLLERVDQ"
     gene            complement(3195..4163)
                     /locus_tag="VIG70_10655"
     CDS             complement(3195..4163)
                     /locus_tag="VIG70_10655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8251058.1"
                     /translation="MKLSILDQSPVIGTLGASRAIAETIALARRADELGYHRYWLAEH
                     HAIAALADPCPEVLLARLGAETRRLRIGSGGVLLPYYSAFRTAESFRMLEALYPGRID
                     LGIGRAPGGDQRTALAVGGGRFPDAAHFPQQVADLAALLEEKSLPIRLQPEVDTVPEV
                     WLLGSSDYSGALAAELGLPFAFAHFINPRGGDVVAREYRDKFKSTGKKQPRVMVCTFA
                     IIAESDDEAERLAAPIDLRRLHMALNIDSPVPTPEEAAKHRYSDEERRYVLSQRPRAV
                     IGGPQKARRELQEMVERYGADELMVLTITGDYDSRRRSYELLIDAF"
     gene            4370..4705
                     /locus_tag="VIG70_10660"
     CDS             4370..4705
                     /locus_tag="VIG70_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251059.1"
                     /translation="MTQITIRFKQEVQSFVNTLFQCLVLALVLGGIGGTIYKVIAPEG
                     WVATAFGRSFSAGAASLGSLALVAGLAWFSRSWGAADNRSLFSHFLVYGFAAAGILYL
                     VQLWTKGTI"
     gene            4805..5074
                     /locus_tag="VIG70_10665"
     CDS             4805..5074
                     /locus_tag="VIG70_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251060.1"
                     /translation="MGQLTEDRAQPKLPTTTSRLIAVAVSLVGEAQSVMLEMKCSHAD
                     LREYCFGLREPSAEEFDRLVELIVVEQRKMLEQNRELLAQARARE"
     gene            complement(5077..5574)
                     /locus_tag="VIG70_10670"
     CDS             complement(5077..5574)
                     /locus_tag="VIG70_10670"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEY8251061.1"
                     /translation="MRAYVITFFVALVLGVAGGVALGVFVYPYIFLADIVATDKVDKP
                     APGQPERKVLARGTFVHANPSDPIHYGKGRVTVYEGLLHLETDFEVGPGPKFHVYLVP
                     EKEVVPSTNVERTMFVDLGRLKAFKGSQNYEVPAGIEIAKFGSVVIWCEQFGVLISPA
                     KLAPD"
     gene            complement(5577..6455)
                     /locus_tag="VIG70_10675"
     CDS             complement(5577..6455)
                     /locus_tag="VIG70_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY8251062.1"
                     /translation="MKTFLLMLGSAVCGVVIFVAVVYGYFYYQWSQAPGATLRFPGTT
                     GKTVSVLPPMAPEGERFHGPTGSIMGEFSRAAQQTALDAGPGKLVGKVTSGGQPLVGL
                     RLRLALNGAAMSQWATSGADGTYEVAVPYGKYRVDGYMLDSASTDRLLRGKINAPQYS
                     PPFRDTITVAEGKPGAGLDFSFVDPVRKTGPSGEVSAAQPVIVTWEPYPNAAAYRLQL
                     VEQSDPRDFETHKRVFDWPKRPVVSGTSANLAEHGVSLKKGHYYAVELEALDARQRPL
                     AESPRLSSAPDFRVSP"
     gene            complement(6452..7516)
                     /locus_tag="VIG70_10680"
     CDS             complement(6452..7516)
                     /locus_tag="VIG70_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8251063.1"
                     /translation="MKRIAQALLVLAFILSGSVGVAADYPAPQEGTWVVRDFKFHTGE
                     TLPELKLHYRTVGDKSGEPVIIMHGTTGSGAGFLAPGFAGELFGPGQALDAKRYFIIL
                     PDAIGHGRSSKPSDGLKMKFPRYNYDDMVDLQHRLVTEHLGVKHARLVMGNSMGGMET
                     WIWAQKYPGFMDAAAPMASTPTEMASRNWMTRRLIIDSIRNDPEWMNGEYTKQPRSAQ
                     FASVFFGIATSGGDQALYKAAPTRAKADALLDARLKAPFPGDANDALYQWDSSRDYNP
                     SGGLERIEATVLVINAADDERNPPHTGVMDREIKRVKNGRVFLIPASENTAGHGTTGQ
                     ARWWKDELAKLLQSAPRRGQ"
     gene            complement(7542..8630)
                     /locus_tag="VIG70_10685"
     CDS             complement(7542..8630)
                     /locus_tag="VIG70_10685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8251064.1"
                     /translation="MHDSLKLGFFIQPVHPPSRAYADVLREDREAVVLADRLGYREAF
                     IGEHLVDSAETITSSLAFIASLADACPSITFGTGVLPLANYHPVMAAAQVAMVDHLLG
                     GRLVLGIGPGVRGDAEAIGDLGSDRNRKTREALDHMCRIWSGEPPYRIEGEFYRTTTE
                     RSLDATIGVGVAVRPLQRPHPPIAITSIRPDGRGPHVAGARGWTGISATYVGAHVVRA
                     QIQAYLEGRRSAGLSTDPSGWRVARSIFVADDEATARRYAHREDGAHGFYFHVMRTKL
                     ARAGALDVMRDFPGQPDSELSIQRCLERLVIAGTPQSVADRILEFRHDVGAFGTLLYT
                     GHDWSDPALARRSMELVANEVWPRIGPS"
     gene            complement(9332..10138)
                     /locus_tag="VIG70_10690"
     CDS             complement(9332..10138)
                     /locus_tag="VIG70_10690"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY8251065.1"
                     /translation="MNNGEQQYLDLVGEVLERGARKTDRTGTGTLSVFGRQLRFALDP
                     GFPLLTTKKLHLKSILTELLWFLRGDTNVRWLQERGVTIWNEWADENGDLGPVYGYQW
                     RHWRTPGGGEIDQIRNVISSLKAKPDSRRHIVSAWNPGDVDRMALPPCHLLFQFYVAA
                     GRLSCQMYQRSADLFLGVPFNIASYAALTLMVAQVTGLKPGEFVLTLGDAHLYLNHLE
                     QAREQTSRQPRAFPRLRLNPAVKELLEFRYEDFTLEAYDPHPAIKAPIAV"
     gene            complement(10139..10930)
                     /locus_tag="VIG70_10695"
     CDS             complement(10139..10930)
                     /locus_tag="VIG70_10695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8251066.1"
                     /translation="MAKEQPVPGYNVVHIIGQGGQAQVYLAEREHDLLRVALKVLNRA
                     LRNDRVFLERFVREYKLVASLDSAYVARIYDQGFSGDYPYIAMEFLPSGTLAARIREG
                     LTTRAALRVATQIAHALDAIHSRGIVHRDLKPANILFRADGRPVLVDFGLARDMRIDS
                     TLTVAGQFLATPRYMSPEQCLGRPVDARSDLYSLGAVLYEMITGKKIYDGANSAEVIA
                     MHVNAPPPKLADLLSMHQPLLDRLLAKKPEDRFQSAAEVLAEIGP"
     gene            complement(10985..11614)
                     /locus_tag="VIG70_10700"
     CDS             complement(10985..11614)
                     /locus_tag="VIG70_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8251067.1"
                     /translation="MDAVEIETGPRPGAAVIWLHGLGADGHDFEPIVPELRLPKPVRF
                     VFPHAPVRPVTINQGMRMRAWYDIFQFGGGPEDEAGIRASQKLVEKLIEAEKGKKIVL
                     AGFSQGGAIVLQAALRHPERLAGVLALSTYLPLAATLEKERSEANQDLPIFMAHGQLD
                     DIIPVGRAEASRDALARLGYNVEWHAYPMPHSVCAPEIEDISRFLSKIL"
     gene            complement(11625..12164)
                     /locus_tag="VIG70_10705"
     CDS             complement(11625..12164)
                     /locus_tag="VIG70_10705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8251068.1"
                     /translation="MVSLRILRSLAAALLLIAGCAGTARTPEVRFEPTPFDVAQAMLE
                     LASVGAGDLVADLGCGDGRIVIEAARRFGARGLCVDIDPRRIAEARRNAAAAGVAERI
                     TFLNQDLFTVELRGVTAVMLFLSPGFNLKLRPKLERELGPGARVVSHWHDMGDWQPSR
                     TVRIRSDGRDRPVYLWTMP"
     gene            12186..12578
                     /locus_tag="VIG70_10710"
     CDS             12186..12578
                     /locus_tag="VIG70_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251069.1"
                     /translation="MKRILVADVPQMDERYSAAFAGCEVVFARTMAEARQALGAGSCA
                     VVVTGVYFDDSRMFDLVRELRSHDVHAKLPIVCVRGRQGLAAVTTRTLEMTVKALAAN
                     DFLDLVQFGDEEAGNAALRAAVQRFLKD"
     gene            complement(12579..12977)
                     /locus_tag="VIG70_10715"
     CDS             complement(12579..12977)
                     /locus_tag="VIG70_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251070.1"
                     /translation="MTKRILVADTPEGDGRISAILAGYDLVFVRTLGEAQRALDHQRF
                     ALVLVGVHFDDSRMFDLLRHVQAVGGPPGCAVICMRSQHFQSPAITIEGLEIAAKALG
                     CNLFLDLTWYADDATGNSAVRMMLEALLSP"
     gene            complement(13054..>13164)
                     /locus_tag="VIG70_10720"
     CDS             complement(13054..>13164)
                     /locus_tag="VIG70_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEY8251071.1"
                     /translation="VFAAVPYCIDLIGGPYIETHESVVKAFRPKSATRQK"
BASE COUNT         2044 a         4356 c         4551 g         2213 t
ORIGIN      
        1 ccgcgcgagc gggtgctcgt ggtgctcggc tttcccgaca tcggcgctgc cggcgatgcg
       61 gtgccgcaga tcctcgccgc ggggccgatc gcctgcgagg gactcgacga ggcgatcatc
      121 ggcgggctgc gcagccgcaa cctgcgtctc gacgacatcg cgctcctgcc gcctggaaaa
      181 gcgtggctga tggtcgagtt cggagcggat tctccggccg aggccgttgc gcgggccaag
      241 agcatcggcg gcatgctcat cacggatcgc gtcctcatga gcaagctttg gacgatccgc
      301 gagaccggcg cctcggcgac cgcgctcaac ctgggcggcc agggcgtcga cccggtggtc
      361 ggatgggaag acgcggcggt ggacccgatg cgcctcggcg actacctgag ggaattccag
      421 gcgctggtcg agcgcttcgg ctaccgcacc tcgctctacg gccacttcgg cgacggctgc
      481 atccacgcgc gcatcaactt cgagctgcgc acgccggcgg ggctcgcgca ctggcggcgc
      541 ttcctcaccg aggcggcgct gctcgtggtg aagtacggcg gctcgctctc gggcgagcac
      601 ggcgacggcc aggcgaaggg cgagctcctg ccgctgatgt tcgggcccga gctgatgcag
      661 gcgttccgcg agttcaagcg cgcatgggac ccgcacaacc ggatgaaccc gggcaagctg
      721 atcgacgcgc gtcccttcga cgccgacttg cggctcggac cggaatacag gcctgtttct
      781 ctgaaaacga aattctcatt caggagcgag gtcggcgacg ggttcgtccg cgcaaccgag
      841 cactgcatcg gcatgggcaa gtgccgcagc cgcgaaggcg gcgcgatgtg cccgagctac
      901 cgcggcacgc acgaggagcg ctactcgacg cgcgggcgtg cgcgcctgct cgccgagatg
      961 ctgcgcggcg aggtgatcac cgagggctgg gcgagcaccg aggtgcagga agcgctcgac
     1021 tggtgcctcg cctgcaagag ctgccgcagc gaatgcccga cgcataccga catggcagcc
     1081 tacaaggcgg agttcctgtc gcactactac gagacccacc gccgcccgcg ccaggcctgg
     1141 tcgatgggcc gcatcgggga gtgggcgccg atggcctcga ggttttccgg ggttgcaaat
     1201 cttctttcct cacttgcagt ttcaaagcgc gtggccggtg tcgatctcac gagaaagctg
     1261 ccgaaattcg cgccgcgcac cttccgctcg cagttcaagc cgaatggctc cggcgatccg
     1321 gtcgtgctgt tcgacgacac gttcaacaac cactttcgcc cggggaccgc cgccgcggcg
     1381 cagaagctgc tcgctcgcgc gggctgcgcg gtcgagttgc cggcgcggca cgtgtgctgc
     1441 ggccggccgt attacgacta cggcatggtg gacgaggcaa agcgcgcgct cgaacgcgtg
     1501 ctcgagatcc ttccttcgga ccgtccgatc gtggtgctcg agccgggctg cctctcggtg
     1561 ttccgcgacg agctacgcca gctcctgccc gacgatccgc gcgctgcaag gctcgcatcc
     1621 aacgttttct cgctgggcga gatgttgcag aagagaaact tcaaaggaac gagccacggc
     1681 cgggtgttca tgcatgcgca ctgccaccag aaggcgctgt ggggcacggc tgcggacctc
     1741 gccatcctga aaagcagcgg ctgcgaggta ctggctcccg acaccgggtg ctgcggcatg
     1801 gcgggctcgt tcggctaccg gccgcagcac gccgagacgt cgcgccgcat cgccgggctc
     1861 gcgctgctgc ccgcgctcga cgcgtcgcgc gatgcggtgg tggtggcgaa cggcttctcc
     1921 tgccgcgagc agatcgaggc gctgtcgggc cgcgcgacgc tccaccttgc ggagctgctc
     1981 gcgccgcgat gagcctggaa gtcctcctct acctcgccgc ggtgtgcgcg ctttccgggt
     2041 tcgtgcacgg cgcgatcggc ttcggcttcc cgctggtggc gacgccgttg gtcgcgctgg
     2101 tgatcgacat gaggtccgcc atcacgctgc tcgcgccggt gaccgtcgtc ctggtcgtga
     2161 tctcggcgct gcgcggcggc ggcctgggcg ggctgctgcg ggacttctgg ttccttccgg
     2221 tcggaatcgc ggccggcgcg tggctcggca cgcgcatcct gctcgccgtg ccgcccgagc
     2281 cgttcaccct ggtgctcgcc ttgatgctta tcttctatct gaatgtcgac cggctcggcc
     2341 gcggccacag cgagaccgtg gttcgcctgc gcgcggtgtt cggcatcgcg ttcgccttcg
     2401 tggccggggt cttcgaggcg gtggcgaacg tcgccggccc ggtgctgctc gtgtacttca
     2461 tgctgctcgg cgccgcgccc cagcagatcg tgcagacgct gaacctgtgc ttctcggtgg
     2521 ggaagggctc gcaggtggcg acgctcgcgg cctcgggcgc cttgagcgcc gcgacctggg
     2581 ccgcggtggc gggcctttcc gtgcccgcgg tggcggcgct cgcagtcggc atgcgattgc
     2641 gcgaccgcat cgatgcgcaa gcctaccgcc gctggttgcg caaggcgctg tgggtgatgg
     2701 cgatcctgct gctggcgcag tttgcccaca gtgcgctcgc ctccgagccg ctgttcaagg
     2761 cgatcgagga gtatcgcgaa ggcgccgccg tcgagctggt cgagcgcgga ggcgtcgact
     2821 tgaacgcgcg caatgggagc ggcgataccc tcgtgcaccg tgcggtggag accggcatgc
     2881 gccagctcct gcaggcgctg ctcgccgcgg gcgcggattc gcgcgcgcgg gcgaaggacg
     2941 gggagaccgc gctccacctg gcggcgctgc atcccgagcc ggtgttcacc gacctcctgc
     3001 tcgccgccgg cgccgatccg cgggcgcgca ataccgacgg cgagtcgccg ctgcactggg
     3061 ccgcgctcag cggccatatc gtcgtggtgc agcgcctcgt tgcgcacggc gcggacgcgc
     3121 ggatcaggaa cggcaagggt ctcactgcgc gcgactacgc gctcagccag gagcactccg
     3181 agatcgcgaa gctcttagaa cgcgtcgatc agtagctcgt aggagcgccg gcgcgagtcg
     3241 tagtcgccgg tgatggtgag gaccatcagc tcgtcggcgc cgtagcgttc gaccatctcc
     3301 tgcagctccc gcctggcctt ttgcgggccg ccgatgaccg cgcgcggccg ctgagacagg
     3361 acgtagcggc gctcctcgtc gctatagcga tgcttcgcag cctcctcggg cgtcggcacc
     3421 ggcgagtcga tgttgagcgc catgtgcagg cggcgcaggt cgatgggcgc ggccaggcgc
     3481 tcggcctcgt cgtcgctttc ggcaatgatc gcgaaggtgc agaccataac tcgtggctgt
     3541 ttcttgccgg tgcttttgaa tttatcccga tactcgcgcg cgacgacgtc gccgccgcgc
     3601 gggttgatga agtgggcgaa agcgaacggc agcccgagct cggcggcgag cgcgccgctg
     3661 tagtccgagg agccgaggag ccacacctcg ggcacggtgt cgacttcggg ctgcagccgg
     3721 atcggcaaag acttctcttc cagaagcgca gccaggtccg ccacctgctg cgggaagtgc
     3781 gccgcatcgg ggaacctgcc gccgccgacc gcgagcgcgg tgcgctggtc gccccccggc
     3841 gcgcggccga tgccgaggtc gatgcgtccg ggatagagcg cctcgagcat gcgaaagctc
     3901 tccgcggtac gaaacgcgct gtagtagggc aggagcacgc cgcccgagcc gatgcgcagg
     3961 cgccgggtct cggcgccgag gcgggcgagc agcacctcgg ggcagggatc ggcaagcgcc
     4021 gcgatggcgt gatgctcggc gagccaatag cggtggtagc cgagctcgtc cgcgcggcgg
     4081 gccagcgcga tcgtctccgc gatcgcccgt gatgcgccga gcgtgccgat gaccggcgac
     4141 tgatcgagaa tcgagagctt catgtcggtg tttttgtcgc ccttacccga cagtcaaaag
     4201 aaccatgcga tcaaggcggc ggcctcgccc atgacatttg taggtcgtgg aagcccgaca
     4261 gacacccggg tggaaatctg tcacgaatgc gcgacaacct gttgtttgga ttggacgacg
     4321 gatctctggc acggcgattg caatataggg gcgcgcgagg aacgccgaaa tgacccagat
     4381 caccatccgg ttcaagcagg aagtgcagtc tttcgtaaat acgttgttcc agtgcctcgt
     4441 gctcgcgctc gtgctcggcg gcataggcgg cacgatctac aaggtgatcg cgcccgaagg
     4501 ctgggtcgcg acggccttcg gccgcagctt ctcggccggc gcggcgtcgc tcggctcgct
     4561 cgcgctggtc gccggccttg cctggttctc tcgcagctgg ggcgctgcgg acaaccggtc
     4621 gctcttttcg catttcctgg tgtacggctt cgcagccgcc gggatcctgt atctcgtgca
     4681 gctctggaca aaaggaacaa tctagctcaa gcggtcggtc ccaaaagatc gactcctccc
     4741 cggggcccgt cctcccccct acaggcgggc cccgtttttt ttgtctaacc ccggagacca
     4801 gggaatggga cagctcaccg aggatcgcgc gcagcccaag cttccaacca ccacttcccg
     4861 cctgatcgcc gtcgcggtct cgctcgtcgg cgaggcgcaa tcggtcatgc tggagatgaa
     4921 gtgctcgcac gccgacctcc gcgagtactg cttcgggctg cgggagccgt cggcggaaga
     4981 gttcgaccgc cttgtcgagc tcatcgtcgt cgagcagcgc aagatgctcg agcagaaccg
     5041 ggaattgctg gcgcaggcgc gcgcgaggga atagagctag tccggcgcga gcttcgccgg
     5101 cgaaatcagc accccgaact gctcgcacca gatcaccacg gatccgaatt tcgcgatctc
     5161 gatcccggcg ggaacctcgt agttctggct gcccttgaag gccttgaggc gcccgaggtc
     5221 gacgaacatc gtgcgctcca cgttggtcga gggcacgacc tccttctccg gcaccaggta
     5281 gacatggaat ttcgggcccg gcccgacctc gaagtccgtc tcgaggtgca gcagcccctc
     5341 gtacacggtg acgcgcccct tgccgtagtg gatcggatcg gacggattgg cgtggacgaa
     5401 cgtgccgcgc gcgagcacct tgcgctccgg ctgcccgggc gccggcttgt cgaccttgtc
     5461 ggtcgcgacg atgtcggcga ggaagatgta ggggtagacg aagacgccga gcgcgacccc
     5521 gccggctacg ccgagaacca gcgcgacgaa gaatgtaatg acgtaggcgc gcacggctag
     5581 ggagagaccc ggaagtcggg cgccgaagaa aggcgcggcg attccgccag cggccgctgc
     5641 cgcgcatcga gggcctcgag ctcgaccgcg tagtaatgac ctttcttcag gctcacgccg
     5701 tgctcggcga ggttcgcgct cgttcccgaa acgaccggac gcttcggcca gtcgaacacg
     5761 cgcttgtggg tctcgaaatc gcgcgggtcg ctttgctcca cgagctgcag gcggtatgcg
     5821 gctgcgtttg gatagggctc ccaggtcacg atgaccggct gcgccgccga tacctcgccg
     5881 gacggtcctg tttttcgcac cggatcgacg aagctgaaat cgagccctgc gccgggcttg
     5941 ccttcggcca ccgtgattgt gtcgcggaac ggcggcgagt actgcggcgc gttgatcttg
     6001 cctctcaaca gcctgtcggt gctggcggaa tcgagcatgt agccgtcgac ccggtatttg
     6061 ccatagggaa ccgcaacctc gtaggtcccg tcggcgccgc tcgtcgccca ctggctcatc
     6121 gccgcgccgt tgagcgcgag ccgcaggcgc aggccgacga gcggctggcc gcccgaggtc
     6181 accttcccga cgagcttccc cggacccgcg tcgagcgccg tttgctgcgc tgcccgggaa
     6241 aattcaccca tgatgctgcc ggtcggcccg tggaagcgct cgccctccgg cgccatcggc
     6301 ggcagcacgc tgaccgtttt tcccgtggtg ccgggaaagc gcagcgtcgc ccccggggcc
     6361 tgcgaccact ggtaatagaa atagccgtac accacggcga cgaagatgac gacgccgcag
     6421 accgccgagc ccagcatcag gaggaaggtt ttcattgccc ccggcgcggc gcgctctgca
     6481 gcagcttggc gagctcgtcc ttccaccagc gcgcctgccc ggtggtgccg tggccggcgg
     6541 tgttctcgct cgccgggatg aggaacacgc ggccgttctt cacgcgcttg atctcgcggt
     6601 ccatcacacc ggtatgcggc gggttgcgct cgtcgtcggc cgcgttgatg acgagcaccg
     6661 tcgcttcgat gcgctcgagg ccgccggacg ggttgtaatc gcgagacgag tcccactggt
     6721 acagcgcgtc gttcgcgtcg ccggggaacg gcgccttgag tcgcgcgtcg agcaacgcgt
     6781 cggccttggc gcgggtgggc gccgccttgt agagcgcctg gtcgccgccg ctggtggcga
     6841 tgccgaagaa caccgaggcg aactgcgcgc tgcgcggctg cttggtgtac tcgccgttca
     6901 tccactcggg gtcgttccgg atcgagtcga tgatcagtcg ccgcgtcatc cagttgcggc
     6961 tcgccatctc ggtcggcgtc gacgccatcg gcgccgccgc atccatgaag ccgggatact
     7021 tctgcgccca gatccaggtc tccatcccgc ccatcgagtt gcccatcacc aggcgcgcat
     7081 gcttcacgcc gaggtgctcg gtcaccaggc ggtgctgcag gtcgaccatg tcgtcgtagt
     7141 tgtagcgcgg gaatttcatc ttcaggccgt cggagggctt gctcgagcgc ccgtggccga
     7201 tcgcgtcggg caggatgatg aaatagcgct tcgcatccag cgcctgcccg gggccgaaca
     7261 gctcgcccgc gaaccccggc gcgagaaagc ccgcccccga cccggtcgtg ccgtgcatga
     7321 tgatcaccgg ctcgccgctc ttgtcgccga cggtgcggta gtgcagcttc aattccggca
     7381 gcgtttcgcc ggtgtggaac ttgaagtcgc gcacgaccca cgtgccttcc tgcggcgcgg
     7441 gatagtcggc ggcaacaccg acgctgccac tcaggatgaa agccagaacc agcaacgctt
     7501 gcgcgattcg cttcatgacg acctcctctg gtgactcacc gtcagcttgg cccgatccgc
     7561 ggccagacct cgttcgcgac cagctccatc gagcgccggg cgagagcagg atcgctccag
     7621 tcgtgaccag tatagagcag cgtgccgaac gcgccgacgt cgtgtcggaa ttcgagaatc
     7681 cggtccgcga cgctctgcgg ggttcccgcg atcaccaggc gctcgaggca gcgctggatc
     7741 gagagctcgg agtcgggctg gccgggaaag tcgcgcatca cgtccagcgc gccggccctg
     7801 gcgagcttgg tcctcatgac gtggaaatag aagccatgcg cgccgtcctc gcgatgcgca
     7861 tagcggcgcg ccgtggcctc gtcatcggcc acgaagatgc tgcgcgccac gcgccacccg
     7921 gagggatcgg tggagaggcc tgccgagcgc cggccttcca ggtaggcctg gatctgggca
     7981 cgcacgacgt gcgcgcctac gtaggtcgcg gagatcccgg tccagccgcg cgctccggca
     8041 acatgcggcc ctcggccatc cggccggatg gaggtgattg cgatgggcgg atgcggcctt
     8101 tgcagtgggc ggacggcgac gcccaccccg atcgtcgcat cgagcgaacg ctctgtcgtg
     8161 gtgcggtaga actctccttc gattcggtac ggcggctctc cgctccagat gcggcacatg
     8221 tggtcgagcg cttcccgggt cttgcggttg cggtccgagc cgaggtctcc gatggcctct
     8281 gcgtcgccgc gaacgccggg ccctatccca agcacgagcc ggccgcccag caggtggtcg
     8341 accatcgcga cctgcgccgc cgccatcacc gggtggtagt tggcaagggg gagcacgccg
     8401 gtgccgaagg tgatcgatgg gcacgcatcg gccaggctcg cgatgaaagc gaggctcgag
     8461 gtgatcgtct cggctgagtc gacgagatgc tcgccgatga aggcctcgcg gtagccgagt
     8521 cgatcggcga gcacgaccgc ctcgcggtcc tcgcgcagaa cgtccgcgta ggcgcgcgac
     8581 ggcgggtgaa ccggctggat gaagaatcct agcttcagag agtcgtgcat ggcgggtctc
     8641 ctggtttgtc ggcatcaccg accgggaccc ccaaacaaaa agcccccgcc ggctcgccga
     8701 caggggcttg ggtatgagct attccaagcg ctagccggcg cggtcctccc ggacgacgac
     8761 tacgacgctt cgctgctgaa tagcttcact tctcatgcaa cgcacgctat cacgcgtagc
     8821 acgggccatc aatgtcctcg tcatgtcttg gccctacccc gcagcagctc tgcgatcaag
     8881 gcagcgcgtc gctcgcgagt cgcctgggcc ttggcagccc gaatgtgctc tcctgcatcc
     8941 ctccgccatc tctcggaggc agccaaccac gcggctgcca ggccctcact cttcgagagg
     9001 aagtcctcca gttccttcgg cgtagatgaa tctgcgagcc gaagcccgag gacgacacgg
     9061 cctccgacat tcaagcgtgc tgtcttgcaa aagggagccg tgaactccac gaaccagtca
     9121 tcggttcgct tgccccaagc tttgatcgtg cggcgcccga aaggatgccc attggcggta
     9181 ccttcgatca cggtcgttcc ggcaaggccc caaccctgca cgtgccgacc cggaatcacg
     9241 acatagaccg gcaggcgcgg atccttgcgc tggagttgcg ttgcgagtcg cacttcaggt
     9301 cgagcaccca tgacgcctaa cgtgacccgg ctcagaccgc gatcggggcc ttgatcgcgg
     9361 gatgcgggtc gtaggcttcg agcgtgaagt cttcgtagcg gaactcgagc agctccttca
     9421 ccgccgggtt caggcgcagc cggggaaatg cccggggttg gcgggaagtc tgctcgcggg
     9481 cttgctcgag gtgattcagg tagaggtgcg cgtcgccgag cgtgaggacg aactcgcccg
     9541 gcttgagacc ggtgacctgc gccaccatca gcgtgagcgc ggcgtaggag gcgatgttga
     9601 aaggcacgcc gaggaagagg tcggcgctcc tctggtacat ctggcacgag agcctgccgg
     9661 ccgcgacgta gaactggaac agcaggtggc acggcggcag cgccatgcgg tcgacgtcgc
     9721 cggggttcca ggccgagacg atgtggcggc gcgaatcggg ctttgccttc agggagctga
     9781 tgacgttgcg gatctggtcg atctcgcctc cgcccggcgt gcgccaatga cgccactggt
     9841 agccgtagac cgggccgagg tcgccgttct cgtccgccca ctcgttccag atcgtcacgc
     9901 cgcgctcctg cagccagcgc acgttcgtgt cgccgcgcag gaaccacaac aactcggtga
     9961 ggatcgactt gaggtgcagc ttcttggtcg tcaggagcgg gaagcccggg tcgagcgcaa
    10021 agcgcagctg gcggccgaac accgagaggg tgccggtgcc ggtgcggtca gtcttgcgcg
    10081 cgccgcgctc gagcacttcg ccgaccaggt cgaggtactg ctgctcgccg ttgttcatct
    10141 acgggccgat ctcggccagg acctcggccg cgctctggaa gcggtcttcc ggcttcttgg
    10201 cgagcaggcg gtcgagcagc ggctggtgca tggagagcag gtcggcgagc ttcggcggcg
    10261 gggcgttgac gtgcatcgcg atgacttcgg cggagtttgc gccgtcgtag atcttcttgc
    10321 cagtgatcat ttcgtaaagc accgcgccga ggctgtagag gtccgagcgc gcatcgaccg
    10381 ggcggccgag gcactgctcg gggctcatgt agcgcggggt ggcgaggaac tgcccggcga
    10441 ccgtgagggt ggagtcgatc ctcatgtcgc gggcgaggcc gaaatcgacc agcaccgggc
    10501 ggccgtcggc gcggaacagg atgttggcgg gcttcaggtc gcggtgcacg atgccgcgcg
    10561 agtggatcgc gtcgagcgcg tgcgcgatct gcgtcgcgac gcgcagcgcg gcgcgcgtgg
    10621 tgagtccttc gcggatgcgc gcggccagcg tgcccgaggg caggaattcc atcgcgatgt
    10681 agggatagtc gccgctgaat ccctggtcgt agatgcgcgc gacgtaggcg ctgtcgaggc
    10741 tcgccaccag cttgtactcg cgcacgaagc gctcgaggaa cacccggtcg ttgcgcagcg
    10801 cgcgattgag caccttcagc gcgacgcgca gcaggtcgtg ctcgcgctcg gcgagataca
    10861 cctgcgcctg cccgccctgg ccgatgatgt gcacgacgtt gtagcccggg acgggttgct
    10921 ccttcgccat cgggctattt tgccattttc accatggcag gcagtacgga gggataaatc
    10981 gagcttagag aatctttgag aggaagcgcg agatatcctc gatctcgggg gcacataccg
    11041 agtggggcat cgggtaggcg tgccattcga cgttgtagcc cagccgggca agcgcatctc
    11101 tcgaggcctc ggcgcgcccg accggaatga tgtcgtcgag ctgcccgtgc gccatgaaga
    11161 tcggcaggtc ctggttggcc tcgctgcgct ccttctccaa ggttgccgcg agcggcaggt
    11221 aggtggaaag cgcgaggacc ccggcgagcc gctccggatg gcgcagcgcg gcctgcagca
    11281 cgatcgcccc gccctgcgag aaacccgcta ggacgatctt cttgcccttc tcggcctcga
    11341 tcagcttctc gaccagtttt tgagaagcac ggattccggc ctcgtcttcc gggccgccgc
    11401 cgaactggaa gatgtcgtac cacgcgcgca tgcgcatgcc ctggttgatc gtgaccgggc
    11461 gcaccggcgc gtgcgggaag acgaagcgca ccggcttggg caacctgagc tcgggaacga
    11521 tcggctcgaa gtcgtgcccg tcggcgccca ggccgtgcag ccagatcacc gccgcgccgg
    11581 gcctcggccc ggtctcgatt tccactgcgt ccatgacgcg cagtttaggg catcgtccag
    11641 aggtagaccg gccggtcgcg gccgtcgctg cggatgcgca cggtgcggct cggctgccag
    11701 tcgcccatgt cgtgccagtg cgagaccacc cgcgcgccgg ggccgagctc acgctcgagc
    11761 ttggggcgaa gcttcaggtt gaatcccggc gacaggaaca gcatcaccgc ggtgaccccg
    11821 cgcagctcga ccgtgaacag gtcctggttg aggaaagtga tgcgctcggc gacgcccgcc
    11881 gcggcggcgt tgcgccgcgc ctcggcgatg cgccgcgggt caatgtcgac gcacaacccg
    11941 cgcgcgccga agcggcgcgc ggcctcgatc acgattcggc cgtcgccgca gccgaggtcc
    12001 gcgaccaggt ccccggcgcc gacgctggcg agctcgagca tcgcctgcgc cacgtcgaac
    12061 ggcgtcggct cgaaccgcac ctcgggcgtg cgcgcggtgc cggcgcaccc ggcgatcagc
    12121 agcagggcag cggccaggct gcgcaggatt ctcaggctta ccatgtcgtt acgaccaggg
    12181 gtgggatgaa gcggattcta gttgccgatg tgccgcagat ggacgagcgc tattcggccg
    12241 cgttcgcggg ctgcgaggtc gtgttcgcgc gcaccatggc ggaagcgcgt caggcgctcg
    12301 gcgccggctc ctgcgccgtg gtcgtgaccg gggtgtactt cgacgattcg cgcatgttcg
    12361 acctggtgcg cgagttgcgc agccacgatg tccacgcgaa gctgccgatc gtctgcgtgc
    12421 gcggccgcca gggattagcc gcggtcacca cccgcacgct cgagatgacc gtcaaagcgc
    12481 tcgcggcgaa cgactttctc gatctggtgc agttcggcga cgaggaggca ggcaacgccg
    12541 cgctgcgcgc cgcggtacaa cgtttcctga aggactaatc agggactaag cagcgcctcg
    12601 agcatcatgc ggaccgcgga gttgccggtc gcgtcgtcgg cataccaggt caggtcgagg
    12661 aacaggttgc agccgagcgc cttcgcggcg atctccaggc cctcgatggt gatcgcgggc
    12721 gactggaagt gctggctgcg catgcagatc accgcgcagc ccggcggccc gccgaccgcc
    12781 tgcacgtggc gcagcaggtc gaacatgcgc gaatcgtcga agtgcacgcc gaccaggacg
    12841 agcgcgaagc gctggtggtc gagcgcgcgc tgcgcctcgc cgagggtgcg cacgaacacg
    12901 aggtcgtagc ccgccaggat ggccgagatg cggccatcgc cttccggcgt gtccgcaacc
    12961 aggattcgct tcgtcatgta gggagggcgg cgagggggcg ttcatgctat cgcccgatcc
    13021 ggcgtccctt tctttacgac agatagtccg gtcttacttc tggcgggttg cggacttagg
    13081 ccgaaaggcc ttcaccacgc tctcgtgggt ctcgatgtac ggaccgccga tcagatcaat
    13141 gcagtagggc acggcggcga acac
//
LOCUS       DATBSZ010000162         7740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2142640, whole genome shotgun sequence.
ACCESSION   DATBSZ010000162 DATBSZ010000000
VERSION     DATBSZ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142640"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..928)
                     /locus_tag="VIG70_10725"
     CDS             complement(62..928)
                     /locus_tag="VIG70_10725"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEY8251072.1"
                     /translation="MNPRKKLRELLSGTRYVVVPGAYDTLSARLAQGAGFDAVYLTGG
                     GYSRANGYPDIGLLTQTEVTQWISRTVDAVDIPVIADMDAGYGNALNVIRSVREYEKT
                     GVAAFHLEDQVSPKKCGHYEGKQVVSRAEMVGKIKAAVDTRRDADMVIIARSDARAIE
                     GLQASIDRVNAYLEAGADVGFVEAPQSRDELAAAARQLKKPALVNIFEGGKTPVLPAR
                     ELEAMGYRIGIYPSQTHRAAIAAAKRVLAVLKRDGDTAAIEGELATFNDREEAVNSRR
                     WRDLETKYLKDV"
     gene            complement(925..3549)
                     /gene="acnA"
                     /locus_tag="VIG70_10730"
     CDS             complement(925..3549)
                     /gene="acnA"
                     /locus_tag="VIG70_10730"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY8251073.1"
                     /translation="MSIDGRATRRALNAETDYYSLEAAETLGLAGVSRLPCSLKVVLE
                     NVLRQHAEGRSDGSDIAAVAGWLATRRAEREIAFRFTRVLMPDSSGVPLLGDLAAMRD
                     AMIRLGGEPMRLNPSVPVDLVVDHSVMVDAYGVPDAVSRNQALELKRNAERYAFLRWG
                     AQAFDNLRIVPPGNGILHQINLEYLARVVWTTDRVAYPDSVLGMDSHTAMINGLGVLG
                     WGVGGLEGGAGALGESVAMLVPEVIGCRLVGRPRPGVTSTDIVLTVTQTLRRHKVVDK
                     FVEYFGPGVAELSLPDRATIANMSPENGATMGFFPVDAETLRYLRLTGRDEAQVALVE
                     AYCKAQGLWRDAGTPPAEFTAIVEIDLSAVEPSVAGPSRPQDRVPLREAPAAFQKLGK
                     NNSGKGVKDGDVVIAAITSCTNTSNPTVMIGAGLLARNAVARGLKVRPGIKTSLSPGS
                     RVVADYLAKSGLQASLDALGFQLAGFGCMTCMGNSGPLPAEVAEAVEQGVTAVAVLSG
                     NRNFEGRIHPSVRANFLASPPLVVAYALAGSVLVNLEHEPLGTGRDGRPVYLRDLWPD
                     GGEIRRIIDAVVTPSLHRERYATMLEGTPEWRALEGKTGTTYAWSPGSTFIRRPPFFE
                     DMGAKPVESADIRGARVLGLFGDMFTTDHISPIGVISKGTPAADYLLSLGVAPADFVN
                     YAARRLNHDVMIRGTFANVRVQNEMTPGVEGSSTVHYPDGERLSIPAAAERYRKDGVP
                     LVVIAGAEYGAGSSRDWAAKGTRLLGVRAVIAESFERIHRSNLVGMGVLPLQFPAGVT
                     RRTLALKGDELVDIAGIFGALAPRGELDCTIVRADGSRHKLKLLSRLDTRRDVDYFRH
                     GGLLHFVLRHRLSNEGPA"
     gene            complement(3546..4100)
                     /locus_tag="VIG70_10735"
     CDS             complement(3546..4100)
                     /locus_tag="VIG70_10735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY8251074.1"
                     /translation="MSEAVLVLDMTRDSIAPEARAVKMRRAVLPRTAAYLAWARGKGM
                     PVVFVCSARRRTDKWFLTFWELANEIWQPGQQPIPELFQPDKDTVVHKRRYSGFFGSD
                     LEITLRELEVASLQLIGWSTSLAVATTAIDAWQLGFPTTVVSDLTIAHAWGGHTVEDN
                     QKWSLDYIAAMAKSKIVRSDELMQ"
     gene            complement(4097..4804)
                     /locus_tag="VIG70_10740"
     CDS             complement(4097..4804)
                     /locus_tag="VIG70_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY8251075.1"
                     /translation="MKPWDGIISEEDQKATYAAGFGRPSSVGRRPALLIIDVQYRTVG
                     TSPKPILEAIKEFPTSCGDVGWNAMRNIVLLLAEFRRNEWPVLYPHVAPKNKATDGGR
                     LAEKVPAIMDIAAKGYEFPAEIAPREGDVLIPKKHPSAFFATPLASHLIDLGADTLVV
                     TGCTTSGCVRGSVVDAFAYNFRAFVPHDAVYDRCATSHAVNLFDMAAKYADVATTAEI
                     MERLRPLSRAQLKSASA"
     gene            4894..5799
                     /locus_tag="VIG70_10745"
     CDS             4894..5799
                     /locus_tag="VIG70_10745"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8251076.1"
                     /translation="MSGTPDLNLLPVFEALLRHGNVTRAAAHMGLTQSAMSSALGRLR
                     RQLGDPLFVNTRGGMLPTPRALELAPSLTDALAMVRGALGVREAFDPRRTTRSLRVYM
                     TDVGETVLLPTLMRHLHQHGPAMRLETAQLPAAELAVRMETGDIDLAVGYVPQLRDKI
                     RRARLFEEHYVCMTRPDHPLGRRSALTLKEFLSARHVLISSMGSGHQQLERALAEHGV
                     EENVALRVPHFMVVPLIVAGTDLIVSLPSQVAKAWTHLVKMKVHPFPIRIPRFDVSLY
                     WHPRVENDAANRWLRGALLELFGDE"
     gene            5796..6791
                     /locus_tag="VIG70_10750"
     CDS             5796..6791
                     /locus_tag="VIG70_10750"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8251077.1"
                     /translation="MSAAKTPVFLLTGFLGSGKTTLLNRALKEPSLANTAVVVNELGE
                     IGLDQVVIAQATDNVVLLDAGCLCCANTGALHETLADLDGRRARGEIPPFERVIIETS
                     GAADPAPLLNVLLGHPLVTGAYAFEATVCTVDAQHFLASRTEYPELDKQAAIAERLYV
                     TKRDLADPQAVLDFLRELNPEAEILASPAELFSSYDGARKFKVSLQGPIRSRAHFSGI
                     RAHAFRPPPPVTWAGWSAWSRLAREEFGARLIRVKGLLQIEETIAFVQGVQGVFHPPQ
                     RFKDWPDDDRENRLVCISRDIPEEDLALTLPALNTPAGTQAIYSMQDLRERYAKQ"
     gene            6788..>7740
                     /locus_tag="VIG70_10755"
     CDS             6788..>7740
                     /locus_tag="VIG70_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEY8251078.1"
                     /translation="MSDFVLRGGTLVFPDKAPEKKDVLVKGGKIEKLLAPGASVSNSS
                     PGVAEQSAAGLHVFPGLIDCHVHFGMGEKITEYSTETANAAQGGFTTVLGYFLNNEAY
                     GDVFRRELDHAVARAHVDFGFHFSTANELHIKELGEYVRDYGVTSFKYFMNFKGEEGR
                     YLGLDGTDDGFFYDLLKESARVGRPIVVCHTENIEIVNRERRKIQAAGGSTLKDWSAS
                     KPAITESEPALRAMYLAQELGARVYFPHISSRKALDVIRLWRQRFKDVIVETCPHYLT
                     HTEDSDLGGMGKANPPFRTKDDLEALWEAIFDGTVDLVASD"
BASE COUNT         1219 a         2683 c         2563 g         1275 t
ORIGIN      
        1 agcgcgagat aaaagggcac gtaaggcacc gcgcgcatgt tctcgcacag cgtgatacgc
       61 attaaacgtc tttcaggtat ttcgtctcca gatcccgcca gcggcgcgag ttcaccgctt
      121 cttcccggtc gttgaaggtc gcgagctcgc cttcgatcgc cgccgtgtcg ccgtcgcgct
      181 tgagcacggc aagcacccgc ttggccgcgg cgatggcggc gcggtgggtc tgggacgggt
      241 agatcccgat gcggtagccc atcgcctcca gctcgcgcgc cggcaggacc ggggtcttgc
      301 cgccctcgaa gatattgacg agcgccggct tcttgagctg gcgcgccgcg gcggcgagct
      361 cgtcgcgcga ctgcggcgcc tcgacgaagc ccacgtcggc gccggcttcc aggtaggcgt
      421 tcacccggtc gatcgaggcc tgcagtcctt cgatggcgcg cgcgtcggag cgcgcgatga
      481 tcaccatgtc ggcgtcgcgc cgcgtatcca ccgcggcctt gatcttgccg accatctcgg
      541 cgcggctgac gacctgcttg ccctcgtagt ggccgcattt cttcggcgac acctggtcct
      601 cgaggtggaa tgccgcgacg ccggtcttct cgtactcgcg cacgctgcgg atcacgttga
      661 gcgcattgcc gtagcctgcg tccatgtcgg cgatcaccgg gatgtcgact gcgtccaccg
      721 tgcgcgagat ccactgcgtg acctcggtct gcgtgaggag cccgatgtcg ggatagccgt
      781 tggcgcgcga atagccgccg ccggtgagat agacggcgtc gaaccccgct ccctgcgcga
      841 gccgcgcgct gagcgtgtcg taggcgccgg gcactacgac atagcgcgtt ccggaaagca
      901 gctcgcgcag cttcttcctt ggattcatgc tggcccctcg ttcgaaaggc ggtgccgcag
      961 cacgaagtgc agcaggccgc cgtggcggaa atagtcgacg tcgcgccgcg tatcgaggcg
     1021 cgagagcagc ttcagcttgt gacggctgcc gtcggcgcgc acgattgtgc agtcgagctc
     1081 gccgcgcggc gccagcgcgc cgaagatgcc ggcgatgtcg acgagctcgt cgcccttcag
     1141 ggcgagcgtc ctgcgcgtca cgccggccgg gaactgcaat ggcagcacgc ccatgccgac
     1201 caggttcgag cggtggatgc gctcgaagct ctcggcgatc accgcgcgca cgcccagcag
     1261 gcgcgtgccc ttcgccgccc agtcgcgcga cgagcccgcg ccgtactcag cgccggcaat
     1321 cactaccagc ggcacgccat ccttgcggta gcgctcggcg gcggcgggaa tcgacaggcg
     1381 ctcgccgtcc gggtagtgca cggtgctgct gccttcgacc cccggcgtca tttcgttctg
     1441 cacgcgcacg ttggcgaacg tgccgcggat catcacgtcg tggttcaggc gccgcgccgc
     1501 gtagttgacg aagtcggccg gcgcgacgcc gagcgaaagg aggtagtccg cggccggcgt
     1561 gcctttcgag atcacgccga tcggcgagat gtggtcggtg gtgaacatgt cgccgaacag
     1621 cccgagcacg cgcgcgccgc gaatatcggc ggactcgacc ggcttcgcac ccatgtcctc
     1681 gaaaaacggc ggccgccgga tgaacgtgct gcccggcgac caggcgtatg tggtgccggt
     1741 cttgccttcc agcgcccgcc actcgggcgt gccttcgagc attgtcgcgt agcgctcgcg
     1801 atggagcgac ggcgtgacga ccgcatcgat aatgcggcgg atctccccgc catcgggcca
     1861 gaggtcgcgc aggtataccg gccggccgtc gcggccggtg ccgagcggct cgtgctcgag
     1921 gttcaccagc acgctgcccg ccagcgcgta cgccactacg agcggcggcg aggcgaggaa
     1981 attcgcccgc accgacggat ggatgcggcc ctcgaagttt cgattgcccg agagcaccgc
     2041 taccgcggtc acgccctgct ccaccgcctc ggccacctcg gccggcaagg ggccggaatt
     2101 tcccatgcag gtcatgcagc cgaacccggc gagctggaag ccgagcgcgt cgagcgacgc
     2161 ctgcaggccg ctcttcgcca ggtagtcggc gaccacgcgc gagccgggcg agagcgaggt
     2221 cttgattccg gggcgcacct tcagcccgcg cgccaccgcg ttgcgcgcca ggaggcccgc
     2281 gccgatcatg accgtcgggt tcgaggtatt ggtgcagctc gtaatcgccg cgatgacgac
     2341 gtcgccgtcc tttacgcctt ttccagaatt atttttccct agtttctgaa aggctgcggg
     2401 tgcctcgcgc agcggcaccc ggtcctgagg ccggctcggc ccggcgacgc tcggctccac
     2461 cgcagaaagg tcgatctcga cgatggcggt gaattcggcc ggcggcgtgc cggcatcgcg
     2521 ccacaggccc tgcgccttgc aataagcctc gaccagcgcg acctgcgctt cgtcgcgccc
     2581 ggtaaggcgc agatagcgca gcgtctcggc gtcgaccgga aagaagccca tggtcgcgcc
     2641 gttctccggg ctcatgttgg cgatcgtggc gcggtcgggg agcgagagct cggcgacgcc
     2701 gggaccgaag tactcgacga acttgtcgac caccttgtgc cgccgcagcg tctgcgtcac
     2761 cgtgagcacg atgtcggtcg aggtcacgcc gggccgcggt cttccgacca gccggcagcc
     2821 gatgacttcg ggaaccagca tcgcgaccga ctcgcccagc gcgcccgcgc cgccttcgag
     2881 cccgccgacg ccccagccga gcacgcccag gccgttgatc atcgcggtat ggctatccat
     2941 gcccagcacg gagtcggggt aagcaacacg gtcggtcgtc cacaccacgc gcgcgaggta
     3001 ctcgagattg atctggtgca ggatgccgtt gcccggcgga acgatgcgca aattgtcgaa
     3061 ggcctgcgcg ccccagcgca ggaacgcgta gcgctcggcg ttgcgcttga gctctagcgc
     3121 ctggttgcgg ctcaccgcgt cgggcacgcc gtacgcgtcg accatcaccg agtggtcgac
     3181 gaccaggtcc accggcaccg acggattgag ccgcatcggc tcgccgccga ggcggatcat
     3241 cgcatcgcgc atcgcggcga ggtcgccgag cagcggcacg cccgaggagt cgggcatcag
     3301 cacgcgcgtg aagcggaacg cgatctcgcg ctcggcgcgc cgcgtggcga gccaccccgc
     3361 caccgccgcg atgtcgcttc cgtccgagcg gccctccgcg tgctggcgca gcacgttctc
     3421 cagcacgacc ttgagcgagc acggcaggcg cgatacgccg gccaggccga gcgtttccgc
     3481 ggcttccaga ctgtaatagt cggtttctgc gttcagcgcg cgtcgcgtcg cgcgcccgtc
     3541 gatgctcatt gcatcaattc gtcagagcgc acgatcttcg acttcgccat ggcggcgatg
     3601 tagtcgagcg accacttctg gttgtcttcg accgtgtgac cgccccacgc gtgcgcgatg
     3661 gtcaggtcgc tcaccaccgt ggtcggaaag ccgagctgcc acgcgtcgat cgccgtcgtc
     3721 gccacggcga gcgaagtgga ccagccgatg agctgcagcg aggcgacttc cagctcgcgc
     3781 agcgtaatct cgaggtcgga gccgaagaac ccgctgtagc ggcgtttgtg caccaccgtg
     3841 tctttgtccg gctggaagag ctcgggaatc ggctgctggc cgggctgcca gatctcgttg
     3901 gcgagctccc agaacgtgag gaaccacttg tcggtgcgcc gccgcgcgct gcagacgaag
     3961 accaccggca tccctttgcc gcgcgcccag gccaggtagg ccgcggtgcg cggcaggacg
     4021 gcgcggcgca tcttcaccgc gcgcgcctcg ggcgcgatcg agtctctggt catgtccagg
     4081 accagaactg cttcgctcac gcgctcgcgc tcttcaattg cgcgcggctg agcgggcgca
     4141 gcctttccat gatttcggcc gtggtggcca cgtcggcgta tttcgccgcc atgtcgaaca
     4201 ggttcaccgc gtgcgacgtg gcgcagcggt cgtacacggc gtcgtgcggc acgaaggcgc
     4261 gaaagttgta ggcgaatgcg tcgaccacgc tgccgcgcac gcagccgctg gtggtgcagc
     4321 ccgtgacgac gagggtatcg gcgccaaggt cgatcaggtg gctggcgagc ggcgtggcga
     4381 agaacgcgct gggatgtttc ttcgggatga gcacgtcgcc ttcccgcggc gcgatctcgg
     4441 ccgggaactc gtaacccttc gccgcgatgt ccatgatggc cggcaccttc tccgcgagac
     4501 ggccgccgtc ggtggccttg ttcttgggcg cgacgtgcgg atagagcacc ggccactcgt
     4561 tgcggcggaa ctcggcgagc agcaggacga tgttgcgcat cgcgttccag ccgacgtcgc
     4621 cgcaggacgt cgggaactcc ttgatcgcct ccaggatcgg tttcggcgag gtgccgacgg
     4681 tgcggtactg cacgtcgatg atgagcagcg cgggccgcct gcctacgctc gagggccggc
     4741 cgaatccggc ggcgtacgtc gccttctgat cctcctcgct gatgattccg tcccagggtt
     4801 tcatgcggcg ctccttcctc gtttcagtct aggcagacct ataattatga gtcaaatcga
     4861 tcgcgttaat tcctttcatc catggtgtag atagtgagcg gaacgccgga tttgaacctg
     4921 ctgccggtgt tcgaggcgct gctgcgccat ggcaacgtca cgcgcgcggc ggcgcacatg
     4981 ggcctcacgc aatccgccat gagcagcgcg ctcggccggc tgcgccgcca gctcggcgat
     5041 cccctgttcg tgaacacgcg cggcggcatg ctgcccacgc cgcgcgcgct cgagctcgcg
     5101 ccttcgctca ccgatgcgct tgccatggtg cgcggcgcgc tcggcgtgcg cgaggcgttc
     5161 gacccgcgcc gcaccacgcg cagcctgcgg gtgtacatga ccgatgtcgg cgagaccgta
     5221 ctgctgccca cgctcatgcg ccacctgcac cagcacggcc cggcgatgcg gctcgagacg
     5281 gcgcagctcc ccgcggcgga gctcgcggtg cgcatggaaa ccggcgacat cgacctcgcc
     5341 gtcggctacg tgccgcagtt gcgcgacaag atccggcgcg cgcgcctgtt cgaggagcac
     5401 tacgtctgca tgacgcgccc cgaccacccg cttggacggc gcagcgcgct gacgctcaag
     5461 gaattcctct cggcgcgcca cgtgctcatc tcctcgatgg gctcgggcca ccagcagctc
     5521 gagcgtgcgc tcgccgagca cggcgtggaa gagaacgtgg cgctgcgcgt gccgcatttc
     5581 atggtggtcc cgctcatcgt cgccgggacc gacctgatcg tcagcctgcc gagccaggtc
     5641 gcgaaagcgt ggacgcacct cgtcaagatg aaggtgcatc cgtttccgat ccgcattccg
     5701 cgcttcgacg tgtcgctcta ctggcatccg cgcgtggaga acgacgcggc caaccggtgg
     5761 ctgcgcggcg cgctgctcga gcttttcggc gacgaatgag cgcggcgaag acgccggtct
     5821 tcctgctcac cggattcctc ggcagcggca agaccacgct cctcaaccgg gcgctgaaag
     5881 aaccttcgct cgccaacacc gccgtggtgg taaacgagct cggcgagatc gggctcgacc
     5941 aggtggtgat cgcgcaggcg accgacaacg tggtgctgct ggacgccggc tgcctgtgct
     6001 gcgcgaacac cggcgcgctg cacgagacgc tggccgacct ggacggccgc cgtgcgcgcg
     6061 gcgagatccc acccttcgag cgcgtgatca tcgagaccag cggcgccgcc gacccggcgc
     6121 cgctcctcaa cgtgctgctc ggccacccgc tggtcaccgg ggcctacgcc ttcgaggcca
     6181 cggtctgcac ggtcgacgcg cagcacttcc tcgcctctcg caccgaatat cccgagctcg
     6241 acaagcaggc cgcgatcgcc gagcgcctct acgtcaccaa acgcgaccta gccgatcctc
     6301 aggctgtttt ggattttctg agagaactga accccgaggc agaaatcctg gcgtcaccgg
     6361 ctgagctgtt ctcgtcctat gacggcgcca ggaaattcaa ggtttcgctc cagggaccga
     6421 tccgctcgcg ggcgcatttc tcgggcatcc gcgcgcacgc ttttcgccct cctcccccgg
     6481 tgacctgggc cgggtggtcg gcgtggtcgc ggctcgcccg cgaggaattc ggcgcgcggc
     6541 tcatccgcgt caaaggcctc cttcagatcg aagaaaccat cgcgttcgtg cagggcgtgc
     6601 agggcgtttt ccacccgccg cagcgcttca aggattggcc cgacgatgac cgagagaatc
     6661 gcctggtctg catctcgcgc gacatcccgg aagaagacct cgcgctcacg ctgccggcgc
     6721 tcaacacgcc ggccggcacg caggccattt attcaatgca ggacctcagg gaaaggtacg
     6781 cgaagcaatg agcgacttcg tattgcgcgg cggcacgctg gtatttccgg acaaggcacc
     6841 cgagaagaag gacgtgctcg tgaagggcgg caagatcgag aagctgctcg cgccgggcgc
     6901 ctcagtttcc aattcttcgc cgggcgtcgc cgagcagtcg gcagcgggcc tgcatgtgtt
     6961 cccgggactc atcgactgcc acgtgcactt cggcatgggc gagaagatca ccgagtactc
     7021 gaccgagacc gccaacgccg cgcagggcgg cttcaccacg gtgctcggct acttcctcaa
     7081 caacgaggcc tacggcgacg tatttcgtcg cgagctcgac cacgccgtcg cgcgcgcgca
     7141 cgtggacttc ggcttccatt tcagcactgc caacgagctc cacatcaagg agctcggcga
     7201 gtacgtccgc gactacggcg tcacctcgtt caagtacttc atgaacttca agggcgagga
     7261 agggcgctac ctcgggctgg acggcaccga cgacggcttc ttctacgacc tgctcaagga
     7321 atcggcgcgc gtcgggcggc cgatcgtggt ctgccacacc gagaacatcg agatcgtcaa
     7381 ccgcgagcgc cggaagatcc aggccgcggg cggcagcacg ctcaaggact ggtcggcctc
     7441 gaagccggcg atcaccgagt ccgagccggc gctgcgcgcc atgtacctgg cccaggagct
     7501 cggcgcgcgc gtgtactttc cgcacatctc ctcgcgcaag gcgctcgacg tgatccgcct
     7561 ctggcgccag cgcttcaagg acgtgatcgt cgagacctgc ccgcactacc tcacgcacac
     7621 cgaggattcc gacctcggcg gcatgggcaa ggccaatccc ccgttccgca cgaaggacga
     7681 cctcgaggcg ctgtgggagg cgatcttcga cggcaccgtc gacctcgtcg cctccgacca
//
LOCUS       DATBSZ010000163        10449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_299104, whole genome shotgun sequence.
ACCESSION   DATBSZ010000163 DATBSZ010000000
VERSION     DATBSZ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10449
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299104"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VIG70_10760"
     CDS             complement(<1..516)
                     /locus_tag="VIG70_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8251079.1"
                     /translation="MSAAIVTGGSAGIGRAIVQALLEKDHEVISLDIQSGESQRKLKH
                     VTVDLTDPVATKRAGQEIAKAHQITTLIHNAGTIRPALLPDVKLEDLEALVNLHLFSA
                     VVLLQSVLPAMKAARFGRVVLVSSRAVLGLPTRTSYAATKAGMLGMARTWALELAPQG
                     ITVNVVAPGPIE"
     gene            complement(513..986)
                     /locus_tag="VIG70_10765"
     CDS             complement(513..986)
                     /locus_tag="VIG70_10765"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY8251080.1"
                     /translation="MNEAELARFVIREARLIDEKRFDEWYELFADDAYYWVPLVPGQT
                     DPLAHNSLAYEDKLLLKLRIERLKQPTAYSQKPQSRCLHVLQAPEVEKSDPARGEYLT
                     RTPFMYTETRGDESQRYAAIAWHTLVGASGGLRIRLKRVDILNADSALPSIQLFL"
     gene            complement(983..2281)
                     /locus_tag="VIG70_10770"
     CDS             complement(983..2281)
                     /locus_tag="VIG70_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY8251081.1"
                     /translation="MARYREDSRALQALVEPDRVHRDVYIDPEVFQLEMERLWSRTWI
                     YVGHASQVPNPGDFITADIAAKPVILVRHTDGKVRLLMNRCAHKGTKVVYDFAGNTGK
                     TFRCPYHAWTYRTDGSLLHIPLKEGYEKTRLHETGAVRGLTPITHETYRGFVFARLAD
                     GPGFREYFGDSLSSIDNLADRSPEGELEIAGGALRFLHNCNWKMFVENLNDTMHPMIV
                     HASSAGTAKKLWEGKPADIPKPMAIEQYAPFANDYKFFDDMGVRVYPHGHGFSGVNFS
                     IHSSYSSIPEYEEAMKRAYGDRAKEILGTVRHNTVYYPSLTIKGAIQSIRVARPLAAD
                     RTVIESWTFRLKGAPAKLLERTVMYNRLINSPMSVVGHDDLHCYRSAQEGLAAQGQEW
                     VSLHRNFDSTELAAKELTCNGTSEISMRNQFRAWREFMAS"
     gene            2305..2769
                     /locus_tag="VIG70_10775"
     CDS             2305..2769
                     /locus_tag="VIG70_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEY8251082.1"
                     /translation="MIVFDLLCGEGHRFEGWFGSAGDFATQRDRDLLSCPTCGSPRVS
                     KVPSATRANLGASEPKPPRRPDATPAKTPEMEGKDPFAIAQMLYSKMLDELLTKSEDV
                     GREFPAEARKIFYKESPARAIRGQATSEEHDDLVDEGIPVARLPLPPTDKLN"
     gene            complement(2865..3242)
                     /locus_tag="VIG70_10780"
     CDS             complement(2865..3242)
                     /locus_tag="VIG70_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8251083.1"
                     /translation="MIAKPAAAVGRVAIYQSDAQGRCVYVNSALCELFEMSEREALGL
                     GWLDRVHPDDRERVAAARRHAASAIPVFYFQYRIRLAARTIWVAAFSTALMEGPTFKG
                     RIGTITDITEAKKHFPEDDAAER"
     gene            complement(3287..3640)
                     /locus_tag="VIG70_10785"
     CDS             complement(3287..3640)
                     /locus_tag="VIG70_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251084.1"
                     /translation="MHYEITEHGNYLRAVIGEAAGLEEFARFYREVQARCATLGLERA
                     LVVVKPEREVSGPDRFSTFASAGFIEGFKLALVCATWALYQSCNKAEMAAARATIHVR
                     AFLQEMEAVSWLKAN"
     gene            3802..4164
                     /locus_tag="VIG70_10790"
     CDS             3802..4164
                     /locus_tag="VIG70_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251085.1"
                     /translation="MLGERQDPNFIVLSKAVAHAHSRLFPDEPYKDPGTLAAIALALT
                     DLIPMYWRDARTGERHQLTELELAAAQFTRAVMELLTVPKARFEAALDTLQVACLDQA
                     RVSLTMRQSPRPAVSPGA"
     gene            complement(4112..5077)
                     /gene="holB"
                     /locus_tag="VIG70_10795"
     CDS             complement(4112..5077)
                     /gene="holB"
                     /locus_tag="VIG70_10795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEY8251086.1"
                     /translation="MHSWNQPILDALALRLERLPHALLIHGPRGVGKLALAERASQFL
                     LCESPAGRPCGSCDGCRWFLGGNHPDFRRLEPEALAKEPPPELAEEAPARRSKQPSIE
                     IKVDQVRELANFLYIGSHRGRLRVAMVHPAEDMNENAANALLKGLEEPPAGAVFILVS
                     HRPAQLLPTIRSRCVAIPVPLPPRDAAVEWLSRQGVKEPQRWLAYAGGAPLRAVEHAA
                     RGAPALAPVDVREGLEPLAEALQKTALDHALLAFGLPAKYQTTSAEVPRGKARDWLAF
                     ARQMGEDRLLCRHPLNPRLFSAAMLAAMPKRLATPPAAGSGASSG"
     gene            complement(5077..5685)
                     /gene="tmk"
                     /locus_tag="VIG70_10800"
     CDS             complement(5077..5685)
                     /gene="tmk"
                     /locus_tag="VIG70_10800"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY8251087.1"
                     /translation="MRGKFLTLEGVDGAGKSTHQKFVADFVAARAPHVIVTREPGGTE
                     LAERLREAILHQAMTPVVETLLIFAARADHVARVIRPALEAGTWVVCDRFSDATLAYQ
                     GAGNGVPAELIERLGTAAHPGLVPDRTLIFDCSYDVSRTRLSGSGKTLDRFEREDRAF
                     FERVRSAYLERARAEPARVRVIDASVAVPEIRKALERNLSDL"
     gene            complement(5682..6680)
                     /gene="mltG"
                     /locus_tag="VIG70_10805"
     CDS             complement(5682..6680)
                     /gene="mltG"
                     /locus_tag="VIG70_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY8251088.1"
                     /translation="MLLKTLLLFVVIAVAAAAGYGAWYLGSPVSIRKYPVEIEIPRGA
                     GFRAAVEQLEKSGVALRRYEFELLARATGKVRDIKAGSYEILQPLTPMQLLQKLTRGD
                     VTQAEIRLIEGWTFAQFRAALDASQYLRHDTKGLDDEQILARLQAPEKHPEGLFFPDT
                     YLFGKGASDLAVLRRSYRAMARHLKEEWEGRDLAAVPYRTPYDGLIMASIIEKETGQA
                     GERDMIGGVLLNRLRIGMRLQVDPTIIYGLGASFDGNLKRSHLVEDGPYNTYTRAGLP
                     PTPIAMPGLASLRSAMRPGKTNAMYYVSRNDGSSHFSRTLEEHNRAVAKYQLRGGR"
     gene            6695..7423
                     /locus_tag="VIG70_10810"
     CDS             6695..7423
                     /locus_tag="VIG70_10810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEY8251089.1"
                     /translation="MECARLNRYGLLAVTGAEARDFLHAQLTNDIANLPADRAALAGW
                     CTAKGRLLASFLVIPSKEGFLLQVARDLAPAVAKRLSMFIFRSKVRIADESDAWAQYG
                     HWNSDSDVAGVEWDGDAVTVRVGERRFLKIGKGLSQPCGDSEETWTLQEIRAGRPLIS
                     AATQDKFVPQMVNFEHLGAINFQKGCYPGQEIVARAQYRGEVKRRMTRVELPKGVELK
                     PGEEFQGGVIVDSAAGEALAVMPV"
     gene            7420..7686
                     /locus_tag="VIG70_10815"
     CDS             7420..7686
                     /locus_tag="VIG70_10815"
                     /inference="COORDINATES: protein motif:HMM:NF027614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HEY8251090.1"
                     /translation="MNYYVYYKVAPERLKEVRLQVEKLFAAIEEHSGVRGRWMRRRDD
                     PTTYMEVYEGVADERGFDALLEREGARLGLQRKVERFISAETPA"
     gene            complement(7661..7942)
                     /locus_tag="VIG70_10820"
     CDS             complement(7661..7942)
                     /locus_tag="VIG70_10820"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY8251091.1"
                     /translation="MYALICFDRPDSAALRDQHRAAHMEFLKQHSGKIVFGGPLKGTP
                     DGPSTGALIVVDCATRKEAEAFIAGDPFHRGGVYESVAVRAFKQVFPQK"
     gene            7958..8872
                     /gene="lsrF"
                     /locus_tag="VIG70_10825"
     CDS             7958..8872
                     /gene="lsrF"
                     /locus_tag="VIG70_10825"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HEY8251092.1"
                     /translation="MADKDDIREGKDFGIGIPQANRAFHVKGGGALDWGMQNRLARIF
                     NPASGRTVMLAFDHGYFQGPTTGLERIDLNIVPLIPYADVLMCTRGALRSVIPASSTN
                     ALVLRCSGGQSILTELSRELIAVDIEDAIRLNAAAITTQIYIGAEYEHQSIKNLVQLI
                     DTGNRYGIPTMAVTGVGAEMKRDERYFGLATRIAAELGAHYVKSYFIEEGFERVVAGC
                     PVPIVIAGGKKLPELDALTMAYKAIDQGAAGVDMGRNIFQSDDPVAMIQAVGAVVHKK
                     EKPEKAFQLYQQFKARNVPDRSRLARSS"
     gene            8835..9494
                     /locus_tag="VIG70_10830"
     CDS             8835..9494
                     /locus_tag="VIG70_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEY8251093.1"
                     /translation="MCLIVLAWRVHPDFPLVVAANRDEFHARPAARAAFWDDHPQILA
                     GRDLEARGTWMGVSRAGKFAAVTNYRGGTEPRAIESRGALVSRFLANGASPGEYMSNL
                     KADLYSGFNLLAADGEELWWTSNRGGKPRRLDPGIYGLGNALLDAPEVDGIKKAFAAD
                     LETGAAVESLFSLLHQARILNPQYGTRCSTVLLKGKRIRYAERAFAADGTEDDTAHFE
                     F"
     gene            complement(9534..9950)
                     /locus_tag="VIG70_10835"
     CDS             complement(9534..9950)
                     /locus_tag="VIG70_10835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8251094.1"
                     /translation="MLRIELMPWKKARAHAAPIRFTVFVEEQGVPIDIELDGQDVLSV
                     HALVFDEEKPVATGRLLPDGHIGRMAVLKDWRRRGIGALMLQSLMERAKERGYPEVAL
                     SAQVQAVPFYRAHGFEAEGEEYLEAGIRHQAMKRRL"
     gene            9974..>10449
                     /locus_tag="VIG70_10840"
     CDS             9974..>10449
                     /locus_tag="VIG70_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY8251095.1"
                     /translation="MKSRWSDAEAGRFTGPLGPRVYTSRLLGQDKSLVLHGGGNTSVK
                     LREKDLFGEEREVLYVKGSGQDLETIEADGFAPLPLAYMQKLPRLPSLSDAQMLNELN
                     THVLRAGAPSPSVETLLHALLPHRYVDHTHADAVLAISNAPDGEKRIREIYGERVAV"
BASE COUNT         1730 a         3426 c         3527 g         1766 t
ORIGIN      
        1 ctcgatcggt ccaggcgcga ccacgttgac ggtgatgcct tgcggcgcga gctcgagtgc
       61 ccaggtgcgc gccatgccga gcatcccggc cttggtcgcg gcgtaggacg tgcgcgtcgg
      121 cagcccgagc accgcgcgcg acgagaccag gaccacgcgc ccgaatctcg cggccttcat
      181 ggccggcagg acgctctgca gcagtacgac cgccgagaag aggtgcaggt tgaccagcgc
      241 ctcgaggtcc tcgagcttca cgtcgggaag cagggcaggg cggatggttc cggcgttatg
      301 gatgagcgtc gtgatctgat gagccttggc gatttcttgg ccggctcgtt tcgtcgccac
      361 ggggtcggtc agatcgacgg tgacgtgctt tagcttcctc tgcgattcgc cggactggat
      421 gtccagcgaa atcacctcat gatctttctc cagcaaagcc tgcacgatcg cgcgcccgat
      481 gccggcgctg ccgccggtga cgatcgcggc gctcacagga agagctggat cgagggcagg
      541 gcggagtccg cgttcaggat gtcgacgcgc ttgaggcgta tgcgcaaccc gcccgacgcg
      601 cccaccagcg tgtgccaggc gatggcggcg tagcgctgcg actcgtcacc gcgcgtctcg
      661 gtgtacatga agggcgtgcg cgtcaggtac tcgccacgcg ccgggtctga cttctccacc
      721 tcgggcgcct gcaggacgtg caggcaccgg ctctgcggct tctgcgaata cgcggtgggc
      781 tgcttcagcc gctcgatgcg cagcttgaga agcagcttgt cctcgtaggc gagcgagttg
      841 tgggcgaggg gatcggtctg gccgggcacc agcggaaccc agtagtacgc gtcatcggcg
      901 aacagctcat accactcgtc gaatcgcttt tcgtcgatga gccgtgcttc ccggatgacg
      961 aatcgcgcga gctcggcctc gttcatgagg ccatgaattc ccgccaggcg cggaactggt
     1021 tgcgcatgga gatttcgctg gtgccgttgc acgtgagttc ttttgctgcc agctccgtgg
     1081 aatcgaagtt ccggtgcagg ctcacccact cctggccctg cgcggcaagg ccttcctgcg
     1141 ccgagcggta gcagtgcagg tcgtcgtggc cgacgaccga catcggcgag ttgatcaggc
     1201 ggttgtacat cacggtgcgc tcgagcagct tcgccggcgc gcccttgagg cggaaggtcc
     1261 aggactcgat cacggtcctg tcggcggcga gcggccgcgc tacgcggatc gactggatcg
     1321 cgcctttgat ggtgagacta gggtagtaca cggtgttgtg gcgcacggtg cccaggattt
     1381 cttttgcccg gtcgccatag gcgcgcttca tcgcctcttc atattcgggt atcgaagaat
     1441 acgaagagtg gatcgagaag ttgacgcccg agaagccgtg gccgtgcggg tagacgcgca
     1501 cgcccatgtc gtcgaaaaac ttgtagtcgt tggcgaacgg cgcgtactgc tcgatcgcca
     1561 tcggcttggg gatatcggca ggcttgccct cccacagctt tttcgcggtg ccggccgagg
     1621 aggcgtgcac gatcatcgga tgcatcgtat cgttcaggtt ctccacgaac atcttccagt
     1681 tgcagttgtg gagaaaccgc agggcgccgc ccgcgatttc caattcgccc tcgggcgagc
     1741 ggtcggcgag gttgtcgatg gaggagagcg agtcgccgaa atattcgcgg aagcccgggc
     1801 cgtcggcgag gcgggcgaag acgaagccgc ggtaggtctc gtgcgtgatc ggcgtcaggc
     1861 cgcgcacggc tccggtttcg tgcagcctgg ttttttcgta gccttccttg agcgggatgt
     1921 ggaggaggga accgtccgtg cggtaagtcc aggcgtgata ggggcagcgg aaggtcttgc
     1981 cggtatttcc ggcgaagtcg taaacgacct tggtgccctt gtgcgcgcag cggttcatca
     2041 gcagccgcac ttttccgtcg gtatggcgta ccaggatgac cggcttggcg gcgatgtcgg
     2101 cggtgatgaa gtcgcccggg ttcggcacct ggctcgcgtg gccgacgtag atccaggtgc
     2161 gcgaccagag ccgctccatc tcgagctgga acacctccgg gtcgatgtag acgtcgcggt
     2221 gcacccggtc gggctcgacc agggcctgca aagcgcggga atcttcccgg tagcgggcca
     2281 tggcggcatt gtagaatcac tcaaatgatt gttttcgatc tgctttgcgg cgagggacac
     2341 cggttcgagg gctggttcgg ctcggccggc gacttcgcca cgcagcgcga ccgggatctc
     2401 ctttcctgcc cgacgtgcgg ctcgccgcgg gtttcgaagg tgccttccgc aacccgggcg
     2461 aacctcgggg cctcggagcc caagccgccc cgtcggccag acgccacgcc cgccaagacg
     2521 ccggaaatgg aaggaaaaga cccgttcgcg atcgcgcaaa tgctctattc gaagatgctg
     2581 gacgagctgc tgacgaagag cgaagacgtg ggccgggaat tccccgccga ggcgcgcaag
     2641 atcttctata aggaatcccc ggcgcgggcg atccgcggcc aggcgaccag cgaggagcac
     2701 gatgacctgg tcgacgaggg cattccggtc gcccggctgc cgctcccgcc gacggacaag
     2761 ctgaactagc ggccaaccgt cgttcccgcg aaggcgggaa cccatgtttt tcaaaaagaa
     2821 aactgggccc ccgccttcgc gggggcgacc tcttcagacc ttccctagcg ctctgcggcg
     2881 tcgtcctcgg ggaagtgctt cttggcttcc gtgatgtccg tgatggtgcc gatgcggcct
     2941 ttgaaggtgg gtccttccat caaggccgtc gagaaggcgg caacccagat cgtcctggcg
     3001 gcgagccgga tccggtactg gaagtagaaa acgggaatgg cggaggcggc gtggcgccgc
     3061 gccgcggcta cgcgctcgcg atcgtcggga tggacgcggt caagccaccc cagcccgagg
     3121 gcctcgcgct cggacatttc gaatagctcg cagagagcgg aattcacgta gacgcagcgg
     3181 ccctgcgcgt ccgactggta aatggcgacc ctgccaacgg cggcggctgg tttggcgatc
     3241 accaggccct tattgggggc agctccctga aacgcaatag gcggggctag ttcgccttca
     3301 gccagctgac cgcttccatc tcctgcagga acgcgcggac gtggatcgtg gcccgggccg
     3361 ccgccatttc cgccttgttg cagctctgat agagcgccca ggtcgcgcac accaacgcca
     3421 gcttgaagcc ttcgatgaag cccgcgctcg cgaaggtgct aaaccggtcc gggcccgaca
     3481 cttcccgttc cggcttcacc acgaccagcg cacgctccaa gccgagggtc gcgcagcggg
     3541 cctgcacctc gcggtaaaag cgtgcgaatt cttcgagtcc tgccgcctcg ccgatgaccg
     3601 cccggagata gttgccgtgc tcggtgatct cgtagtgcat gtcccgacgc tcagcaaagc
     3661 ttgttcccgc tcagggtcgg ctgccttgtg tgtcgcagcc aatgtgtatt atgttaaata
     3721 atgtctcaca gcccattcag ggtccaaccg ggccatcccc cacgatgacc aactggccta
     3781 ttgcgttcct atcctgccct cgtgcttggc gagcgacagg atccgaactt catcgtgctt
     3841 tcgaaggcgg tggcgcacgc ccatagcagg ctgttcccgg acgagccgta caaggatccc
     3901 ggcacgctgg cggcgatcgc cctcgccctc accgacctga tccccatgta ctggcgcgac
     3961 gcgcgcaccg gagagcgcca tcagttgacg gagctcgagc tcgccgcggc gcagttcacc
     4021 cgggcggtga tggagcttct gaccgttccg aaagcacgct tcgaggctgc cctggacacg
     4081 ctccaggtcg cgtgcctcga ccaggcgcgc gtcagcctga cgatgcgcca gagcccgcgg
     4141 ccggcggtgt cgccaggcgc ttaggcatcg ccgccagcat cgcggcggaa aacaatctcg
     4201 ggttgagcgg gtggcggcag aggagccggt cttctcccat ctgccgcgca aaggcgagcc
     4261 agtcccgggc cttcccgcgc ggcacctcgg cgctggtcgt ctggtacttc gcgggcaggc
     4321 cgaacgccag cagcgcgtga tccagggcgg ttttctgcag cgcttcggcg agcggctcca
     4381 gtccttcgcg cacgtccacg ggcgccagcg cgggcgcacc ccgcgcggca tgctccacgg
     4441 cgcgcagcgg cgcgccaccg gcgtaggcaa gccagcgctg cggctctttt acgccttgcc
     4501 gtgaaagcca ctcgactgcg gcgtcgcgcg gcggcaaggg gaccgggatc gccacgcagc
     4561 ggctgcggat cgtgggaaga agctgcgccg ggcggtgcga taccaggatg aacaccgccc
     4621 cggccggcgg ctcctcgagc cccttgagga gcgcgttggc ggcgttctcg ttcatgtcct
     4681 cggcgggatg aaccatggcg acgcgcagcc ggccgcggtg cgagccgatg tagaggaaat
     4741 tcgccagctc gcgcacctgg tccaccttga tctcgatgct gggctgcttg ctgcggcgag
     4801 ccggggcttc ttccgccagc tccggcggcg gctccttcgc aagggcctcg ggctccagcc
     4861 ggcggaaatc gggatggttg ccgcccagga accagcggca tccgtcgcag gaaccgcacg
     4921 gccgcccggc ggggctctca cacagcagga actgggaagc tctctcagcc agcgcaagct
     4981 tgccgacgcc gcgcggccca tggatgagga gcgcatgcgg caggcgctcc aggcgcagcg
     5041 cgagcgcgtc gaggatcggc tggttccagg aatgcatcac aggtccgaga ggttacgctc
     5101 gagcgccttg cggatctccg gcaccgcgac cgacgcgtcg atcacccgga cgcgggccgg
     5161 ctcggcgcgg gcgcgctcga ggtaggcgct gcgcacgcgc tcgaagaacg cccggtcctc
     5221 gcgctcgaag cgatccaggg tttttccaga gccggaaaga cgagttcgtg acacgtcata
     5281 actacagtca aaaatcaggg tgcggtcggg aacaagcccc ggatgcgcgg cggtgccgag
     5341 ccgctcgatc agctcggccg gcacgccgtt ccccgcgccc tggtaggcga gtgtggcgtc
     5401 cgaaaagcgg tcgcagacca cccaggtgcc tgcttcgagc gccgggcgaa tcacgcgcgc
     5461 gacgtggtca gcgcgcgcgg cgaagatgag cagcgtctcc accaccggcg tcatggcctg
     5521 gtgcaggatg gcctcgcgca gccgctcggc gagctcggtg ccgccgggct cgcgcgtcac
     5581 gatcacgtgc ggggcgcgtg cggcgacgaa gtcggcgacg aatttctggt gggtggactt
     5641 gcccgcgccg tcgacgccct cgagggtcag gaatttcccc ctcatcggcc gccgcgaagc
     5701 tggtacttcg ccaccgcgcg gttgtgctct tccagcgtgc gcgagaagtg gctcgagcca
     5761 tcgttgcgcg acacgtaata catggcgttg gtttttcccg gacgcatcgc cgagcgcagc
     5821 gaggcgagcc cgggcatggc gatcggcgtc ggcggcaggc cggcgcgagt ataggtgttg
     5881 taggggccgt cctcgaccag gtggctgcgc ttgagattgc cgtcgaacga ggccccgagg
     5941 ccgtagatga tggtcgggtc cacctgcagc cgcatgccga tgcgcaggcg gttcagcagc
     6001 acgcccccga tcatgtcgcg ctcgcccgcc tgcccggtct ccttctcgat gatcgaggcc
     6061 atgataagcc cgtcgtaggg cgtgcggtag ggcacggcgg cgaggtccct gccctcccat
     6121 tcctccttca gatggcgtgc catggcgcgg tagctccgcc gcagcaccgc gaggtcgctc
     6181 gcgcccttgc cgaagagata ggtgtcggga aagaagaggc cctcgggatg cttttcggga
     6241 gcctgcaggc gggcgaggat ctgctcgtcg tcgaggcctt tggtgtcgtg ccgcaggtac
     6301 tggcttgcgt cgagcgccgc gcggaactgc gcgaaggtcc agccctcgat caggcggatc
     6361 tcggcctggg tgacgtcgcc gcgggtgagc ttctgcagga gctgcatcgg cgtcagcggc
     6421 tgcaggatct cgtagctgcc cgctttgatg tcgcgcacct tgccggtcgc gcgcgccagc
     6481 agctcgaact cgtagcgccg cagcgcgacg ccggattttt cgagctgctc caccgcggcg
     6541 cggaagccgg cgccgcgcgg gatctcgatc tccaccgggt acttgcggat cgacaccggc
     6601 gagcccaggt accacgcgcc gtagccggcg gccgcggcga cggcgataac cacgaagagg
     6661 agaagggtct tcagcaagat aaaatgattc tacgatggag tgcgcgcgct taaatcgcta
     6721 cgggttgctc gcggtgacgg gtgccgaggc gcgtgatttc ctgcacgcgc agctcaccaa
     6781 cgacatcgcg aacctgcctg ccgatcgcgc ggccctcgcc ggctggtgca cggccaaggg
     6841 ccggctgctg gccagctttc tcgtcattcc ttcgaaagaa ggatttctcc tgcaggtcgc
     6901 gcgggatctc gccccggcgg ttgcgaagcg cctgtcgatg ttcatcttcc gctcgaaagt
     6961 caggatcgcc gacgaaagcg acgcctgggc gcagtacggt cactggaatt ccgattccga
     7021 tgttgcgggc gtggaatggg acggcgatgc cgtcaccgtg cgggtgggcg aacgccggtt
     7081 cctgaagatc gggaaagggc tctctcagcc ctgcggggat tctgaagaaa cctggaccct
     7141 gcaggaaatc cgcgccggcc ggccgctgat cagcgccgcc acccaggaca agttcgtgcc
     7201 gcagatggtg aatttcgagc acctcggcgc gatcaacttc cagaagggct gctatccggg
     7261 ccaggagatc gtggcgcgcg cccagtatcg cggcgaggtc aagcgccgca tgacccgggt
     7321 cgagctgccg aagggtgtcg agctcaagcc cggcgaggaa ttccagggcg gcgtgatcgt
     7381 ggacagcgcg gcgggcgaag cgctcgccgt gatgccggtt tgaactacta cgtttactac
     7441 aaggtcgcgc ccgagcggct gaaggaagtc cggctgcagg tcgagaagct cttcgctgcg
     7501 attgaagagc acagcggtgt ccgcgggcgc tggatgcgcc ggcgcgacga ccccacgacc
     7561 tacatggagg tgtacgaggg cgtcgcggac gagcgcggct tcgacgcgct gctcgagcgc
     7621 gagggcgccc ggctcggcct gcagcgcaag gtcgagcgct tcatttctgc ggaaacacct
     7681 gcttgaaggc gcgcaccgcc acgctttcgt agaccccgcc gcggtggaac gggtcgccgg
     7741 cgatgaaagc ctccgcctcc ttgcgcgtcg cgcagtccac cacgatcagc gcgccggtgc
     7801 tcgggccgtc gggcgtgcct ttcagcggtc cgccgaacac gatttttccc gagtgttgtt
     7861 tcaggaattc catgtgggcc gcacggtgtt gatcgcgcag cgccgcggag tccgggcggt
     7921 cgaaacaaat cagggcgtac atggtatgtt ggaagctatg gccgacaaag acgatatcag
     7981 agaaggcaag gacttcggga tcggcatccc gcaggcgaac cgtgcgttcc acgtgaaggg
     8041 cggcggcgcg ctcgactggg gcatgcagaa ccgcctcgcg cgcatcttca acccggcctc
     8101 gggccgcacg gtgatgctcg cgttcgacca cggctacttc caggggccca cgactggcct
     8161 cgagcgcatc gatctcaaca tcgtgcctct catcccctac gccgatgtcc tgatgtgcac
     8221 gcgcggcgcg ctgcgctccg tgatcccggc gtcgtccacc aacgccctgg tgctgcgctg
     8281 ctcgggcggc cagagcatcc tcaccgagct gtcgcgggaa ctgatcgccg tggacatcga
     8341 ggacgccatc cgcctcaacg ccgcggccat caccacgcag atctacatcg gggcggaata
     8401 cgagcaccag tcgatcaaga acctggtgca gctcatcgat accggcaacc gctacggcat
     8461 cccgaccatg gcggtgaccg gcgtcggcgc ggagatgaag cgcgacgagc gctactttgg
     8521 cctcgccacg cgcatcgccg cggagctcgg cgcgcactac gtgaagagct acttcatcga
     8581 ggaaggcttc gagcgcgtgg tcgccggctg cccggtgccg atcgtgatcg ccggcggcaa
     8641 gaagctgccc gagctcgatg ccctcaccat ggcgtacaag gcgatcgacc agggcgcggc
     8701 cggcgtcgac atggggcgca acatcttcca gtccgacgac ccggtagcca tgatccaggc
     8761 cgtaggcgcg gtggttcaca agaaagagaa gccggagaag gcgttccagc tttaccagca
     8821 attcaaagcc cggaatgtgc ctgatcgttc tcgcctggcg cgttcatcct gattttccgc
     8881 tggtcgtcgc ggccaaccgc gacgagttcc acgcccgtcc cgccgcccgg gccgcgttct
     8941 gggacgatca cccgcagatc ctcgccggcc gcgatctcga ggcgcgcggc acctggatgg
     9001 gcgtgtcgcg cgccggcaag ttcgccgcgg tgaccaacta ccgcggcggc acggaacccc
     9061 gcgcgatcga gtcccgtggg gcgttggttt cgcgctttct cgccaacggc gccagcccag
     9121 gggagtacat gagcaacttg aaagccgacc tgtacagcgg attcaacctg ctggcggccg
     9181 acggcgagga gttgtggtgg acctccaacc gaggcggcaa gccgcgccgc ctggacccgg
     9241 gcatctacgg gctcggcaac gccctgctcg acgcgcccga ggtcgacggc atcaagaaag
     9301 ccttcgccgc cgacctcgaa accggtgcgg cggtcgagtc gctgttcagc ctgctccacc
     9361 aggcgcgcat cctgaacccg caatacggca cgcgctgctc gaccgtattg ctgaaaggaa
     9421 agcgcattcg ctacgccgag cgtgccttcg ccgccgacgg caccgaagac gacaccgcgc
     9481 acttcgagtt ctaggcaaaa gccaaaaacg tggtctgtcc ctgtttttcg ccgttagagg
     9541 cggcgtttca tcgcctggtg ccgaatgccg gcttcgaggt attcctcgcc ttcggcttca
     9601 aagccgtgcg cgcggtagaa cgggacggcc tggacctgcg ccgacagcgc gacttccggg
     9661 tagccgcgct ccttcgcgcg ttccatcagg ctctgcagca tgagcgcacc gatgccgcgg
     9721 cggcgccagt ccttcagcac cgccatgcgc ccgatatggc cgtcgggcag caggcggccc
     9781 gtggcgaccg gcttttcctc gtcgaacacg agcgcgtgga cggaaagcac gtcctgcccg
     9841 tcgagctcga tgtcgatcgg cacgccctgc tcctccacga acacggtgaa gcggatcggc
     9901 gccgcgtgcg cgcgggcttt cttccagggc atgagctcga ttcgcagcat gatgcgaaaa
     9961 gtataatcgc cgcatgaaaa gccgctggag cgacgccgag gccgggcggt ttaccggccc
    10021 gctgggaccg cgcgtctata cctcgcggct tctcgggcaa gacaagtcgc tggtgctcca
    10081 cggcggcggc aatacctcgg tgaagctccg cgagaaagac ctcttcggcg aggagcgcga
    10141 ggtcctgtac gtcaagggaa gtgggcagga cctggagacc atcgaagccg acggcttcgc
    10201 gccgctgccg ctcgcctaca tgcagaagct tccccgcctg ccttcgctat ccgacgcgca
    10261 gatgctgaac gagctgaaca cgcacgtgct gcgcgcaggc gcgccctcgc cctcggtcga
    10321 gaccctgctg cacgccctcc tcccgcaccg ctacgtcgac cacacccacg ccgacgcggt
    10381 gcttgccatc tccaacgccc cggacggcga gaagcgcatc cgcgagatct acggcgagcg
    10441 cgtcgcggt
//
LOCUS       DATBSZ010000164         5851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1801016, whole genome shotgun sequence.
ACCESSION   DATBSZ010000164 DATBSZ010000000
VERSION     DATBSZ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801016"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="VIG70_10845"
     CDS             complement(<1..1272)
                     /locus_tag="VIG70_10845"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY8251096.1"
                     /translation="MNAPAQAGLPAFPLKDPKLFRTECYVDGSWVGTSHKINVVNPAN
                     GSVLGTVPRMGAEEARRAIEAAERAWPAWRAKTAKERAVILRKWFDLMMANQEDLAQI
                     LTAEQGKPLTEARGEIAYGASFIEWFAEEGKRAYGDVIPQHQPDKRVLVIKQPIGVCA
                     LITPWNFPNAMITRKAGPALAAGCTVVIKPASQTPYSALAMAELGERAGIPKGVLNIL
                     TGDSKAIGAELCGNPKVRKLSFTGSTETGRVLMRQCADTVKKLSLELGGNAPFIVFDD
                     ADLDAAVEGALASKYRNAGQTCVCANRLYVQDSVYDAFSAKLAEKVKGFKVGAGTEPG
                     VVIGPLIDENGVKKVEAHVADALGKGAKVMLGGKRHALGGLFFQPTVLTNVTPDMLVS
                     HEETFGPVAPLIRFKTEDEVIRLANDTEFGLS"
     gene            1340..2290
                     /locus_tag="VIG70_10850"
     CDS             1340..2290
                     /locus_tag="VIG70_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8251097.1"
                     /translation="MKTLLAAALFFAAATAHAQYPQRPVQLIVPWGAGGGTDATARII
                     GTLLERELKQPFNVVNRTGGSGVVGHDAIAKAPADGYTIGMITVEITMMHHVGLTPLK
                     HTDYTPIGLVNADPAGVNVRVDSPYKSVKDLLAAIKANPGKLKASGTGQGGIWHLAIA
                     GLLKDQGIDPAALPWVPSQGAAPGLQDLVAGGVEVVPCSIPEARAMIDGGKVRALAIM
                     DANPPSLYPNVPTLKKELGTGWQIAAWRVIAAPKGIPAPVQNTLVTALKKVYDSKEYK
                     DFMAKQGYGVVWADPAGTTKFMADSDKNLGAALKAVGLAK"
     gene            2319..2768
                     /locus_tag="VIG70_10855"
     CDS             2319..2768
                     /locus_tag="VIG70_10855"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY8251098.1"
                     /translation="MTGAALVALAAVILWHIQGFPAMPGQRFGPAWFPGSIAAGLGIC
                     GLCLIFAGVRQRSPWVALPGWVARRRPLAGVASVVAGLVFYILAADKLGFHLTGILLL
                     AFWIRVLGASWRVAAIVAVGATVAIHLSFYKMLRIPLPWGLLEQYAF"
     gene            2758..4233
                     /locus_tag="VIG70_10860"
     CDS             2758..4233
                     /locus_tag="VIG70_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY8251099.1"
                     /translation="MLFEAAALVFDPYVLWVILASAAFGLFVGAVPGLTATMATALLV
                     PVTFFMEPVPAIASIVTATAMAIFAGDIPSCLLRMPGTPASAAYTDEAYAMTKKGQGE
                     LALGAGLVFSVVGGLFGTAVLIMAAPALAEVALKFSSFEYFWLVTLGLLCAVFIAFAN
                     PLKGIVSLLLGLLVASVGLDNPAGFPRFTFGNAELAGGLTLIPIMIGMFAVSEIIRSV
                     SRIDEPWEMAQAKIGNVFRGMWDLTKRYRMPVLRGSVLGTAVGVLPGAGADIAAWMSY
                     AVSKKFSKEPEKFGTGHVEGIIEAGASNNSALAGAWVPALVFGIPGDSITAIVIGVLY
                     IKGLNPGPSIFMQNAVSIYAIFMVFILANLLMLPLGIAAIKSAKQLLRTPRHVLMPVI
                     LLFCIVGSFAINNSMFGVVLVLAFGIIGYFMEENGFPVAPAILGMVLGAMLEEHFIRA
                     MIRADGNFIVFFERPIALGLAVAAFLVVFWPLAAWLWRRKP"
     gene            4230..5183
                     /locus_tag="VIG70_10865"
     CDS             4230..5183
                     /locus_tag="VIG70_10865"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY8251100.1"
                     /translation="MIRFALIAVALAAMPAFAQEKIGKPVRLIVGFAAGGSVDLVARL
                     MAERLSPRLGQPVIVENRPGGFVRVAIDAVRGASPDGTTLLLTSSTPLVVTPHAYAKV
                     PYDAQRDLASVARLVNGQQGFALGPMSGARSWPDYVAWVKANPDKAFFASSGVGSMGH
                     LVGVVLANTSGLRIEHVAYKGSGHYNAELMGGQVSAGISGIADAADFHRSGKLRIVAT
                     SGAERSRALPDVPTFRELGVPMDLTLWYGVFAPAGTPPGIVELLGRELVAIVAAPELT
                     ERFVNLGLDPAPAGPKATAEQLAADFARWRPIVQQAGFKLD"
     gene            5183..>5851
                     /locus_tag="VIG70_10870"
     CDS             5183..>5851
                     /locus_tag="VIG70_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY8251101.1"
                     /translation="MVSFSGPFRAPKGSAIRELWPYLSLPDMISFAGGYPSPALFDAE
                     GLHAAMEHAMRAPPAEWLQYANTQGELPLREALSGWMARRGIAASADRILVTTGSQQG
                     FDLILRVMLDPGDTVVVERPTYTTALQALRHAGARVVTVGTDADGMDVDELATVLTPG
                     IKAIYTLPTFGNPTGATLPLARRKRLLELAVRHDVLIIEDDAYGDVRFDGEEVPALAA
                     LARDA"
BASE COUNT          852 a         1953 c         2009 g         1037 t
ORIGIN      
        1 cgacaggccg aactcggtgt cgttggccag ccggatcacc tcgtcctcgg tcttgaagcg
       61 gatgagcggc gcgaccggcc cgaaggtttc ctcgtgcgag accagcatgt cgggcgtgac
      121 gttggtgagc acggtgggct ggaagaagag gccgccgagc gcgtgccgct tcccgcccag
      181 catgaccttg gcgcccttgc ccagggcgtc ggcgacgtgc gcttccactt tcttcacgcc
      241 gttctcgtcg atcagcgggc cgatcaccac gccgggctcg gtgccggcgc cgaccttgaa
      301 gcctttcacc ttctccgcga gcttggcgga aaaggcgtcg tagacgctgt cctgcacgta
      361 aaggcggttc gcgcacacgc aggtctggcc agcgttgcgg tacttggagg cgagcgcgcc
      421 ctcgactgcg gcgtcgaggt cggcgtcgtc gaacacgatg aacggcgcgt tgccgccgag
      481 ctcgagcgag agtttcttca ccgtgtcggc gcactgccgc atcagcacgc gcccggtctc
      541 ggtcgagccg gtaaacgaga gcttgcgcac cttggggttg ccgcacagct cggcgccgat
      601 cgctttcgag tcgccggtga ggatgttgag cacgcctttg gggatgccgg cgcgctcgcc
      661 gagctcggcc atcgcgagcg cggaataggg cgtctggctg gcgggcttga tgaccaccgt
      721 gcagccggcg gcgagcgcgg ggccggcctt gcgtgtgatc atcgcgttcg ggaaattcca
      781 cggagtaatg agcgcgcaga ccccgattgg ctgcttgatc accagcacgc gcttgtcggg
      841 ttggtgctgc ggaatgacgt cgccataggc gcgcttgcct tcctcggcga accattcgat
      901 gaacgaggca ccgtaggcga tctcgccgcg cgcctcggtc aacggcttgc cctgctcggc
      961 ggtgaggatc tgcgcgaggt cctcctggtt cgccatcatc aggtcgaacc acttgcgcag
     1021 gatgacggcg cgctccttcg cggtcttcgc gcgccaggcc ggccacgcgc gctcggcggc
     1081 ctcgatcgcg cggcgcgctt cctcggcgcc catcctgggc accgtgccaa gcaccgagcc
     1141 gttcgcgggg ttcaccacat tgatcttgtg tgaagtgccg acccagctac catcgacata
     1201 acactcggtg cggaagagct tcgggtcttt cagggggaat gccggcaagc cggcctgggc
     1261 gggcgcattc atgggggttt gctccaaaaa aagaatctta cgccttagaa tcgcttgtct
     1321 gacaacccga ggaggagtca tgaaaacgct attggccgcc gcactgtttt tcgcggcggc
     1381 aaccgcgcac gcccagtatc cgcagcgccc ggtgcagctg atcgtgccct ggggcgcggg
     1441 cgggggcacc gatgcgaccg cccggatcat cggcacgctg ctcgagaggg agctgaagca
     1501 gcccttcaac gtggtgaacc gcaccggcgg ctcgggcgtg gtcggccacg acgcgatcgc
     1561 caaggcgccg gccgacggct acaccatcgg gatgatcacg gtggagatca cgatgatgca
     1621 ccacgtcggc ctcacgccgc tcaagcacac cgactacacg ccgataggac tggtgaacgc
     1681 cgatcccgcc ggcgtgaacg tacgggtcga ttcgccgtac aagtcggtca aggacctcct
     1741 cgccgcgatc aaggcgaacc cgggcaagct caaggcctcg ggcaccggcc agggcggcat
     1801 ctggcacctc gcgatcgccg gactgctcaa ggaccaggga atcgatcccg cggcgctgcc
     1861 atgggtaccg tcacagggcg cggcgcccgg gctgcaggac ctcgtcgccg gcggcgtgga
     1921 agtcgtgccg tgctcgatcc cggaggcgcg cgcgatgatc gacggcggca aggtgcgcgc
     1981 gctggcgatc atggatgcga atccgccgtc gctctacccg aacgtgccca cgctgaagaa
     2041 ggaactcggc acggggtggc agatcgcggc gtggcgcgtg attgcggcgc ccaagggaat
     2101 tccggcgccg gtgcagaaca cgctcgtcac agcgctgaag aaggtctacg actccaagga
     2161 gtacaaggac ttcatggcga agcaaggcta cggcgtggtg tgggctgatc ccgccggtac
     2221 cacgaagttc atggccgatt ccgacaagaa cctcggagct gcgctcaaag ccgtagggct
     2281 ggccaagtaa ggatcgacgc atcaaggtca acgatgcgct gaccggcgcg gcgctcgtcg
     2341 cgctcgccgc agtcatcctc tggcacatcc aggggtttcc ggccatgccg ggccagaggt
     2401 tcggcccggc ttggtttccc ggctcgatcg ccgccgggct cggaatctgc gggttgtgcc
     2461 tgatcttcgc cggcgtgcgc cagcgttcgc cgtgggtagc gcttccgggt tgggtggcgc
     2521 gtcgccggcc gctcgccggc gtggcgagcg tggtcgccgg cctcgtgttc tacatcctcg
     2581 ccgccgacaa gctcggcttc cacctcaccg gcattctgct gctcgcgttc tggatccgcg
     2641 tcctcggcgc ttcgtggcgc gtggccgcaa tcgttgccgt gggggccacg gtggcgattc
     2701 acctctcgtt ctacaagatg ctgcgcatcc cgctgccgtg gggattgctg gagcaatatg
     2761 ctttttgaag cggctgcgct ggtgttcgat ccgtacgtgc tgtgggtgat cctggcatcg
     2821 gcggcgttcg gtctgttcgt cggcgcggtt cccgggctta ccgccaccat ggcgacggcg
     2881 ctgctcgtgc cggtcacgtt cttcatggag ccggtgcccg cgatcgcctc gatcgtgacc
     2941 gcgacggcga tggcgatctt cgccggcgac atcccgagct gcctgctgcg catgccggga
     3001 acgcccgcgt ccgccgccta taccgacgag gcctacgcga tgacgaaaaa gggccagggt
     3061 gagctcgcat tgggggccgg cctggtcttt tccgtcgtgg ggggtctgtt tggcactgca
     3121 gtcctgatca tggccgcgcc ggcgctggcc gaggtcgcgc tgaaattctc ctcgttcgag
     3181 tatttctggc tggtgacgct cggcctgctg tgcgcggtct tcatcgcgtt tgccaatcct
     3241 ctcaaaggga tcgtctcgct gctgctcggg ctgctcgtgg cgtcggtcgg cctcgacaac
     3301 ccggcggggt ttccgcgctt caccttcggc aacgccgagc tcgccggcgg cctcacgctg
     3361 atcccgatca tgatcggcat gttcgcggtg tccgagatca tccgcagcgt ctcgcgcatc
     3421 gacgagccct gggaaatggc gcaggcgaag atcggcaacg tgttccgcgg catgtgggac
     3481 ctcaccaagc gctaccgcat gcccgtgctg cgcggcagcg tcctgggcac cgcggtcgga
     3541 gtgcttccgg gcgcaggcgc cgacatcgcg gcatggatga gctatgcggt ttcgaagaag
     3601 ttctccaagg agcccgagaa gttcggaacc ggccacgtcg agggcatcat cgaggcgggc
     3661 gcctcgaaca acagcgcgct cgccggcgcg tgggtgccgg cgctggtatt cggcattccc
     3721 ggcgactcga tcaccgcgat cgtgatcggc gtgctctaca tcaagggcct caatccgggg
     3781 ccgtcgatct tcatgcagaa cgccgtgtcg atctacgcca tcttcatggt gttcatcctg
     3841 gcgaacctgc tgatgctgcc gctcggcatc gccgcgatca agagcgcgaa gcagctgctg
     3901 cggacgccgc gccacgtgct gatgccggtg atcctgctgt tctgcatcgt cggctcgttc
     3961 gcgatcaaca attccatgtt cggcgtggtg ctggtgcttg cctttggaat cattggatac
     4021 ttcatggaag aaaacggctt tccggttgcg ccggcgatcc tcggcatggt gctcggcgcg
     4081 atgctggagg agcatttcat ccgcgccatg atccgcgcag acggaaactt catcgtgttt
     4141 ttcgagcgcc cgatcgccct cgggctcgcg gtcgccgcgt tcctcgtcgt gttctggccg
     4201 ctcgccgcgt ggctatggag aagaaaacca tgattcgatt cgccctcatc gccgtcgctc
     4261 tcgcggcgat gcccgcgttc gcgcaggaaa aaatcggcaa gccggtgcgc ctgatcgtcg
     4321 gcttcgctgc cggcggctcg gtggatctgg tggcgcgcct gatggccgag cggctgtcgc
     4381 cgcgcctcgg gcagccggtg atcgtggaga accggcccgg cggcttcgtg cgcgtcgcga
     4441 tcgacgcggt gcgcggcgcc tcgcccgacg gcaccacgct gctcctcacc tccagcactc
     4501 cgctcgtggt gacgccgcac gcctatgcga aggtgccgta cgacgcgcaa cgcgatctcg
     4561 cctcggtcgc gcgtctggtg aacggccagc agggcttcgc cctcggcccg atgtcgggcg
     4621 cgcgctcctg gcccgactac gtcgcgtggg tgaaggcgaa cccggacaag gcgttcttcg
     4681 cgagctccgg cgtcggcagc atgggccacc tggtcggcgt ggtgctcgcc aacacgagcg
     4741 ggctgcgcat cgagcacgtg gcgtacaagg gctcgggcca ctacaacgcc gagctgatgg
     4801 gcgggcaggt ctcggccggc atcagcggca tcgccgacgc ggcggatttt caccgcagcg
     4861 gcaagctgcg catcgtcgcc acctccggcg ccgagcgctc gcgcgcgctt ccggacgtgc
     4921 cgacgttccg cgagctcggc gtgccgatgg acctgacgct ctggtacggc gtgttcgcgc
     4981 cggccggcac gccgccgggg atcgtcgagc tgctcgggcg cgagctggtg gcgatcgtgg
     5041 ccgcgcccga gctgaccgag cgcttcgtca acctggggct ggatccggcg ccggccgggc
     5101 cgaaggcgac cgcggagcag ctcgccgccg acttcgcgcg ctggcgaccc atcgtgcagc
     5161 aggccggctt caagctggac tgatggtctc tttctcgggt cccttccggg cgcccaaggg
     5221 ctcggccatc cgggagctct ggccgtactt gtcgctgccc gacatgatct cgtttgccgg
     5281 cggctatccg tcgccggcgc tgttcgacgc cgaggggctg cacgcggcga tggagcacgc
     5341 gatgcgcgcg ccgcccgccg agtggctgca gtacgcgaac acgcagggcg agctgccctt
     5401 gcgcgaggcg ctgtcgggat ggatggcgcg gcgcggcatc gcggcgagcg ccgaccgcat
     5461 cctggtaacc accggctcgc agcagggctt cgacctgatt cttcgcgtga tgctcgaccc
     5521 aggcgatacc gtggtcgtgg agcggccgac ctataccacc gcactgcagg cgctgcggca
     5581 cgccggcgcg cgcgtcgtca ccgtcggcac cgacgccgac ggcatggacg tggacgagct
     5641 ggcgacggtc ttgactcccg gcatcaaggc aatctacacg ctcccgacct tcggcaatcc
     5701 tactggtgcg acgctcccgc tcgcgcgacg caagcggctg ctcgagctcg cggtacgcca
     5761 tgacgtcctc atcatcgagg acgacgccta cggcgacgtg cgcttcgacg gcgaggaggt
     5821 gccggcgctc gccgcgctcg cgcgcgacgc g
//
LOCUS       DATBSZ010000165        17213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_94888, whole genome shotgun sequence.
ACCESSION   DATBSZ010000165 DATBSZ010000000
VERSION     DATBSZ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17213
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94888"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..830)
                     /locus_tag="VIG70_10875"
     CDS             complement(48..830)
                     /locus_tag="VIG70_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8251102.1"
                     /translation="MSYQTLLVEERGEHLLLVTLNRPQVLNALNTQMGKDKLDLWTKL
                     AAEPGNVRCVVLTGAGERAFCSGGDLKERDGMTDTAWRAQHEIFERAFMALMDLPLPV
                     IAAVNGHAFGGGLEIALCCDFIYAARSARLALSETRLGLMPGGCGTQNLPRAAGERRA
                     KELILTAQAFEAQQGYDWGVVNRVCDKVVDDALATARTIAENGPLAVRQAKKSIHYGL
                     QMDLATGYRFEIEAYNQLVGTDDRREGVRAFNEKRKPRFQGK"
     gene            complement(841..1800)
                     /locus_tag="VIG70_10880"
     CDS             complement(841..1800)
                     /locus_tag="VIG70_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8251103.1"
                     /translation="MRIAALGAGRMGRGIGHVFAYAGYPVDIIDFKPGSRDLREPVMK
                     EIEGNLRLLSELGLLDERQKKTILGRIHVVPFADGPGALKAADVVFEGVTETLEAKRD
                     AFGRACKHLRPDAIIASTTSSMLADTLAQMVTHPGRFLNAHFLNPAYLIPLVEVSPAK
                     TTDENTFEKIKTLLESAGKVVVRCAPSPGFIVPRLQAAAMNEAARLVEEGVATPEDID
                     KAVRAGFGPRYSAMGLCEFIDFGGLDILYYASSSMAKALDAPRYEPPALVKKLMEEGK
                     RGAREGQGLYDWRGRDLAAYQKDLLGRYVNLFRYLGMLAPPRD"
     gene            complement(1797..2840)
                     /locus_tag="VIG70_10885"
     CDS             complement(1797..2840)
                     /locus_tag="VIG70_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HEY8251104.1"
                     /translation="MKISVIGGGNGAYAAAADLTEQGHEVRMWRRNTGALKSVLTLKD
                     AEGERNVSVSTSVEIGEALRGAELVFMPDPAFTQADNAKRIAPHLADGQVVFLAPGTF
                     GSYVMAKIVRDAGNRADFAFAETGTLPWLTRKHGPATTAITARATRLPTGVFPRKRSE
                     FAFSVIRKAFKSIEPVEDALSGALMNAGPVIHPPLILMNAGPLEHFEKWDIHKEGTQP
                     SIRRVTDALDAERIAARVALGYKAPHFPLADHYRADGDEWMYGRKVHKKLTDSQDWRE
                     RIVLTEHRYMREDVEYGLAFLVSVCEYAGVPCPVARGLLALGSAVVGRDLRQEGRTLE
                     NLGLAKADLKAAL"
     gene            complement(2853..3254)
                     /locus_tag="VIG70_10890"
     CDS             complement(2853..3254)
                     /locus_tag="VIG70_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8251105.1"
                     /translation="MTAKLRHIAISVPELKTAAEFYKKTFGLEEVNYVETPYGDGLSL
                     SDGVINLTLLKFHSDDAAGDERGKDFHGIHHMGFIVDDLEGYSKRVQQNGGRFHRELK
                     GGGGVDFERKFRDPNGVVFDISHKGWVGIKE"
     gene            complement(3251..4378)
                     /locus_tag="VIG70_10895"
     CDS             complement(3251..4378)
                     /locus_tag="VIG70_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY8251106.1"
                     /translation="MRIADARERTVGIGADMRNASIGFGAMTASALALVSDRGLVGYA
                     FDSIGRYGKGALLRERFLPRLLRAKPEALLDAAGLIDPAACARAAMIDEKPGGHGERP
                     GAIGLIEAAAWDLRAKAQDLPLWQSIAQYFGRQAQSQIAVYASCGHFRTDDVLTFEVK
                     NAIDAGYTCVKIKVAGEVESDLKRLERAAASLPRNAIWAVDVNGALEEKKMNPWLDAL
                     EPFKLAWIEEPAPPLDFEHLKACARSSATPLATGENLFSFDDARNLLRYGGLRPERDF
                     VQVDPLLAYGVTEYSKILAAYEAAGWKRKQFLPHAGHLFAAHCVAGLGLGMAEAAPDT
                     SLPYGGYWDGVRVAGGRVTIPDAPGAGYERKANLYALLESL"
     gene            complement(4378..4830)
                     /locus_tag="VIG70_10900"
     CDS             complement(4378..4830)
                     /locus_tag="VIG70_10900"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY8251107.1"
                     /translation="MKDELEAFVLAEARLLDERRFSDWLALFAEDGIYWVPTNPRQAS
                     PQEALSLFYEPKPLLAMRVARLDRPDMHVQSPASRTVHHVSAVEVSGDLEVRSALVVA
                     EWRAGDARWFAARVLHRLRREARGLRIVLKRVDLIDSEAPQRAIAVPF"
     gene            complement(4827..6116)
                     /locus_tag="VIG70_10905"
     CDS             complement(4827..6116)
                     /locus_tag="VIG70_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8251108.1"
                     /translation="MNVADFWRANSVHRAAYVDPEVFALEQERIFRRAWLYVGHESEV
                     PQPGDYVLRRLGPDEVLLVRGEDRSLALLHNRCAHRGARIVAQPAGNARQFRCPYHSW
                     TYALDGRLVGMPLPEGYAEKVELGLARVARVESYRGFVFGSHAAQGPGLAEFLGGLAS
                     AFDNMLDRAPAGTVSRFGGELRLEYRGNWKMFMENAVDLVHPNFVHRSSIEGARQALG
                     AARSQPADPLTEQGAQMFLANGMRAPEWDKVPLHAYPGGHVYMGGFYRSGVIAPERAD
                     PVYERYRAQLVERHGAEKAAAVLAVDRFNNLVWPTVSVNSRFAALRVVQPLAADRTMV
                     EVQCFRLEGADAAMHDLTLQFVNLAASPASLVASDDLEIFERCQRGLQSAASDWIDVR
                     RGALADRRQPDASTLGSGTSELAIRHQFATWLQYMTR"
     gene            complement(6120..6602)
                     /locus_tag="VIG70_10910"
     CDS             complement(6120..6602)
                     /locus_tag="VIG70_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY8251109.1"
                     /translation="MSLERWHRVQQFLFHEARLLDERRLQEWLALYAEDAEYWVPYTW
                     GQKSPKDHVSLFYETLTLLRMRVDRLERELSPLDSPMARVNHHLSNVVVEEGEGEELA
                     ARAYLLFIEHRREEQRWFAGRASWRLRPQGESFLIGAKRVDLLNADQDSGHLRFAVPF
                     "
     gene            complement(6599..7858)
                     /locus_tag="VIG70_10915"
     CDS             complement(6599..7858)
                     /locus_tag="VIG70_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8251110.1"
                     /translation="MREEYRELVGRGRVHRRFYTDPAVFEEERERIFRRVWLWLGHES
                     QLKRPGDFFTARLAGERVIVARHTDGTIHAFHNRCTHRGAEVCLAAAGNARSFVCPYH
                     AWTYRTDGSLASVPLEQGYGAEFRSRMAGLRLEPVARVASYRGFIFGSHAAEGDDLET
                     YLGPEVRAAFDNLVERAPDGEVELAGGKTVQRYRGNWKLQLENSIDLLHPRILHRSAI
                     DAADRVDHGGGPPSIEWDIVKSNGLTLREWDGMQIAAMPRGHCWMAGFLTRAIDENPL
                     QARYRKIMVERHGEKRAAEILSFNRHNTIVYPNLFVNSRVAQIRVLQPVNVDCTEQHG
                     YIFRLKGAPDEMFEASVRMVNTNNSPSSIVTSDDHEIFERIQRSLAGGQHEWVDLSRG
                     LDAEEAAVGTNELPMRNQHRAWAQYMG"
     gene            7865..8572
                     /locus_tag="VIG70_10920"
     CDS             7865..8572
                     /locus_tag="VIG70_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEY8251111.1"
                     /translation="MLILQSAGMHKVSIRPHIVERVVARRGDLHWFTRLDAAKTALLV
                     IDMQNTFCMPGAPGEVPAARAIVPNINRFAAALRAHGVPVVWILHANTVQNGHSDWEV
                     FFGHVVRNADVRRRMAESLAPARQAVWKDLEVAPGDVTLVKNRYSALAHGASTLERVL
                     RNLGVDTVLVAGTKTNVCCDSTARDAMMLDFKSIMVSDCCAALSDEEHLASLEIFIQQ
                     FGDVMTADEALERLAKT"
     gene            8585..9538
                     /gene="dctP"
                     /locus_tag="VIG70_10925"
     CDS             8585..9538
                     /gene="dctP"
                     /locus_tag="VIG70_10925"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY8251112.1"
                     /translation="MKAFKATLFALLAAAGTASAQETTLNAVVFVPRNTTFGEIFVRF
                     VDQVNKEGKGLVQVKLIGGPDAIPTFEQGSALRTGVVDMVSLPPTFYTNVCPECDAQI
                     LPPLQTPQLKKTPFWNVLQKYTNQKVNAQLLTAYGDGIGFHIWTSKPPAGGTLKGMRL
                     RTTPNYTPLFTALGVSLVQTAPGEVLTALERGVVDGYGWPAIGIFDLGWGAHTKYRID
                     PGSYNVIVNVLVNLNKWNALRPDQRAFLNRMGDWLEAENVRWVAERKAADDKKIKEAG
                     IQLVDLGPAHRQLAYDAYWDTLAKRAPEPIKELRAASDALR"
     gene            9535..10152
                     /locus_tag="VIG70_10930"
     CDS             9535..10152
                     /locus_tag="VIG70_10930"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEY8251113.1"
                     /translation="MRRVLEDLVIPAGRGHAFLVPRGAVLRIHLVEDRQVGDCCFFNA
                     NDRREVFHVGQSWALNVMLGTGNAKSFRHFYSKPPRENVMLTVLEDTVRNHWGNMGGR
                     CSKRLYELRDGDRDHRSCQENLAEALAPYGVCGDDIVDIFNVFMNVEMRPDGSFTILP
                     PSAKKGDYIDLRAEMDVLAAVSACPADRNATNDGRAKPLGITIST"
     gene            complement(10144..11037)
                     /locus_tag="VIG70_10935"
     CDS             complement(10144..11037)
                     /locus_tag="VIG70_10935"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8251114.1"
                     /translation="MNVHDIDLNLLRVFDAVLHEKGVTPAAARLGLTQPAVSNALARL
                     RKVFGDALFVRTPRGVDATPFARELAEPVRQALALLESALAHGPGFDPATSTRAFRFY
                     MSDLGQIEFLPPLVERVQRDAPGARLEAVALEVEDIADALAAGALDLAIGFLPGLAAP
                     VRRQALFRDPYLCLMRADHPITTLTKKRFLDASHALVIYRGGHRVIEEALERAGVARR
                     IALRVPHFTVVPMVLERSDLILTLPARVARVFERRGKLKALPPPVPIPSAEVAVHWHE
                     RFEADPGNRWLRQQVVELFSG"
     gene            11123..12073
                     /gene="dctP"
                     /locus_tag="VIG70_10940"
     CDS             11123..12073
                     /gene="dctP"
                     /locus_tag="VIG70_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY8251115.1"
                     /translation="MRKSVLLALLLAPAVVAAQETALRVVSAFAENTQYVKNLMPMIQ
                     NLNTEGKGSLQLHFIGGPKAMPPFEVGNAVRTGVVDVAMTTGAFYTNIMPEADALKLT
                     QMPHSELRKNGGHDLINRIWNEKANMQYLGRVIDYTPFHLYLTKKIDKPDLTGLKLRI
                     TPVYRDFFQALGATVMQTAPGEVYTALERGVVDGYGWPINGIFDFNWHEKTKFRVDPG
                     FYSAEVSLVMNLDKWKALSAAQRELLTKHVIALEAGNDSWKKVNEDDIRKQKEAGIQV
                     ITFDAATAKQYYDRAYEVAWTNAIKASPQYGPQMRKLFSK"
     gene            12070..12525
                     /locus_tag="VIG70_10945"
     CDS             12070..12525
                     /locus_tag="VIG70_10945"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY8251116.1"
                     /translation="MIWYRKLLHGLAGAAALLLGAMALLVTLDVLMRNVGVGTLAWVN
                     EVSEYSLPLATLLIAPWLLHRNEHVRLDVLLVSLPRAASRLLEKACDVVGIAICAVFV
                     WYSLRLILDSSRIGSMVVKTLSIPEWWQYALVPVCFSLLAVEFARRLRA"
     gene            12522..13838
                     /locus_tag="VIG70_10950"
     CDS             12522..13838
                     /locus_tag="VIG70_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY8251117.1"
                     /translation="MTWWLSAIVLFGVLTALFLVGLPAAFAFFGINIVGAWFYLGKDA
                     GLVQLIRNGIVSITSFSLTPIPFFILMGEVLFHTGVAMKAIDAIDRVIWRVPGRLSVV
                     AVVAGTVFSAISGSTIATTALLGSLLLPQMLRRGYHPNMAMGPIMAIGGVDMLIPPSA
                     LTVLLGSLAGISISQLLIAGIVPGVLLSLMFIGWIVLRAVADPSLAPSFEHEELPLWE
                     RLRPLLLYVMPLVLIFVVVVAAMTAGWATPTEAAAIGAVATVAVAWIYRALTWRNLMQ
                     ALLGTAGISGIILFIIIGATTFAQILTFSGATNGMVEAIRDAGFASWTVLIAMLLVLL
                     LLGCFVDQVSMMLICLPFFMPLVALYGFDPVWFGVLFLICMQLGLLTPPFGMLLFTMK
                     SVAPAGIEMRQVWHAVTPYVVMGVFALVAVMMFPPLATWLPKLMFK"
     gene            13841..15910
                     /locus_tag="VIG70_10955"
     CDS             13841..15910
                     /locus_tag="VIG70_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEY8251118.1"
                     /translation="MERSFKEEVEKLRLGEGETFHGEGILAVTKALLQSGVSYVGGYQ
                     GAPVSHVMDVLNDARDILDELGVHVEANANEAGAAAMLGASINYPMRGAVAFKSTVGT
                     NVASDALSNLASAGVKGGALIVLGEDYGEGASIIQERSHAFAMKSQIWLLDPRPNLPK
                     IVEMVEQGFELSEASSTPVMLELRIRACHVQGSFKTRKNQAPQFSKKAFLQNPDFDYG
                     KICLPPATYAQEKQKVDVRWPAAVRFIRERKLNEFFDGEQSDLGILCQGGMYNAVIRA
                     LQMLGLADAFDASRVPIYCMNVTYPLIPEEITEFCKDKKSVLIVEEDQPAYIEDAVLA
                     VLRRAGANSVKVSGKDMLPLAGEYTGEVVLTGVSGFLDRSERVQPILSLKKKASELLA
                     GLPARPPGFCVGCPERPVFSAMKVLEKESGKFHLSADIGCHLFSTLPPFNIGNTVLGY
                     GLGLASNSAVHPMFGKRTVTMMGDGGFWHNGLTSGIANTVFNKNDGILVIMKNGYSSA
                     TGTQELPSSPQHSEHKAPDMSMERALEGIGVGWVKTVDNYRVGEVAKTLKEAMTTDYP
                     GLKVIIAEGECQLERQRKLRPLTSKRLSEGKRTVRVKYGVDEDTCTGDHSCIRLSGCP
                     TLTIKDNPDPLRRDPVATVIDGCVGCGLCGENAHAAVLCPSFYRAEVIQNPNWWDKLW
                     NASSLRK"
     gene            15883..>17213
                     /locus_tag="VIG70_10960"
     CDS             15883..>17213
                     /locus_tag="VIG70_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HEY8251119.1"
                     /translation="MERQFTKEIESLRLGDGETFHGEGILAVTKALLQSGVSYVGGYQ
                     GAPVSHLLDVMVQAKDYLDELGVHVEACTSEASAAAMLGASINYPARGAVTWKSIVGT
                     NVAADALSYLASAGVMGGTLIVLGEDYGEGGVVAQERSHAFALKSLMWLLDPRPNLTT
                     IVDMVERGFELSEVSNTPVMMELRIRACHVRGSFKTRRNLAPDISTTQKLDGPPKEFI
                     YDRLAHPPATFRQEKLKSDVRLPAARKFILEKRLNEIFPGGENVGVIVQGGLFNGLML
                     ALRTLGLADQFGASRIPILALNVVYPLVPEEINGFCEDKKAVLVLEEGQPEFIEQEVA
                     TLLRRARVGAELHGKDCVHMAGEYNVEALVRGLARFLGMHAAHLDTSAGSAWLDSVQG
                     TKKKATALLGALPARPPTFCVGCPERPVFSAMKLVAQDIGRPHVSMDVGCHA"
BASE COUNT         2854 a         5752 c         5765 g         2842 t
ORIGIN      
        1 cgcagcaggc tgccgatgtg atcggcccgg aacgggggcg tcatgcgcta cttcccttgg
       61 aagcggggct ttctcttctc gttgaacgcg cgcacgcctt ccctgcggtc gtcggtgccg
      121 acgagctggt tgtacgcctc gatctcgaag cgatagccgg tggcgagatc catctgcagg
      181 ccgtagtgga tcgatttctt cgcctggcgc acggcgagcg gcccgttctc ggcgatggtg
      241 cgcgcggtgg cgagcgcgtc gtcgaccacc ttgtcgcaga cccggttcac gacgccccag
      301 tcgtagccct gctgcgcctc gaacgcctgg gcggtgagga tcagctcctt cgcgcggcgc
      361 tctccggcgg cgcgcggcag gttctgcgtg ccgcatccgc ccggcatcag gcccaggcgg
      421 gtctccgaca aagcgagccg cgcgctgcgc gccgcgtaga tgaagtcgca gcacagcgcg
      481 atctccaagc cgccgccgaa agcgtggccg ttcaccgcgg caatcaccgg cagcggcagg
      541 tccatcagcg ccatgaacgc gcgctcgaag atctcgtgct gcgcgcgcca tgccgtatcg
      601 gtcatgccgt cgcgttcctt gaggtcgccg ccggagcaga acgccctctc gccagcgccg
      661 gtgagcacta cacagcgcac gttcccgggc tcggcggcga gcttcgtcca cagatcgagc
      721 ttgtccttgc ccatctgggt attgagcgcg ttcaggacct gcggccggtt gagcgtcacg
      781 aggaggaggt gctcgccgcg ctcttcgacg agaagggtct ggtagctcat cgctcgcatc
      841 ttaatcgcgc ggcggggcca gcatccccag gtagcggaag aggttcacgt agcggccgag
      901 caggtccttc tggtaggccg cgagatcgcg gccgcgccag tcgtagaggc cctggccttc
      961 gcgcgcgccg cgcttgccct cttccatcag cttcttcacc agcgcgggcg gctcgtagcg
     1021 cggcgcatcc agcgccttcg ccatcgagct cgacgcgtag tacaggatgt cgaggccgcc
     1081 aaagtcgatg aattcgcaca ggcccatcgc gctgtagcgt gggccgaagc cggcgcgcac
     1141 cgccttgtcg atgtcctcgg gcgtggcgac gccctcctcc accaggcgcg cggcctcgtt
     1201 catcgccgcc gcctgcagcc gcggcacgat gaagccgggc gaaggcgcgc agcgcaccac
     1261 gaccttgccc gcggactcga gcaaggtctt gattttctcg aacgtgtttt cgtcggtggt
     1321 cttggccggg ctcacttcca ccagcggaat caggtaggcc ggattgagaa aatgcgcgtt
     1381 caggaagcgt cccggatggg tcaccatctg cgccagcgtg tcggccagca tcgaggaagt
     1441 ggtggacgcg atgatggcgt ccgggcgcag gtgcttgcag gcacgaccga acgcgtcgcg
     1501 cttcgcctcg agcgtttcgg tgacgccctc gaacacgacg tccgcggcct tcagcgcccc
     1561 ggggccatcg gcaaagggaa cgacgtggat tcgtccgagg atggtctttt tctgccgctc
     1621 gtccagcagg ccgagctcgg acagcagccg caggttaccc tcgatctcct tcatcaccgg
     1681 ctcgcgcagg tcgcggctgc ccggcttgaa gtcgatgatg tcgaccgggt agccggcgta
     1741 ggcgaagaca tggccgatgc cgcggcccat gcgccccgcg ccgagagccg cgatcctcaa
     1801 agagccgctt tcaagtcggc tttcgccagc ccgaggttct cgagcgtgcg gccttcctgg
     1861 cggaggtcac ggccgacgac cgccgagccg agcgcgagca gccctcgcgc gaccggacac
     1921 ggcacgccgg cgtactcgca gaccgagacc aggaacgcca ggccgtactc cacgtcctcg
     1981 cgcatgtagc ggtgctcggt gagcacgatg cgctcgcgcc agtcctgcga gtcggtgagc
     2041 ttcttgtgca ccttgcggcc gtacatccac tcgtcgccgt cggcgcggta atggtcggcc
     2101 agcgggaagt gcggtgcctt gtagcccagg gccacgcgcg ccgcgatgcg ctcggcatcg
     2161 agcgcgtcgg tcacgcggcg aatcgaaggc tgcgtgcctt ccttgtggat atcccatttc
     2221 tcgaagtgct cgagcggtcc ggcgttcatc aggatgagcg gcggatggat gaccgggccg
     2281 gcgttcatca gcgcgccgga gagagcatcc tccacgggtt cgatgctttt gaaggccttg
     2341 cggatcacgg aaaaagcgaa ttcgctgcgc ttgcggggga aaacccctgt gggcaaccgt
     2401 gtcgcccgcg cggtaattgc ggtggtggcc gggccgtgct tgcgcgtgag ccaaggcagc
     2461 gtgccagtct cggcgaaggc gaagtccgcg cgattgcccg cgtcgcggac gatcttcgcc
     2521 atgacgtagg agccgaaggt tccgggcgcg aggaacacga cctggccgtc ggcgaggtgc
     2581 ggcgcgatgc gcttcgcgtt gtcggcctgc gtgaaggcgg gatcgggcat gaacaccagc
     2641 tcggcgccgc gcaacgcttc tccgatttct actgaagtcg aaacggaaac attgcgttcg
     2701 ccctccgcgt ccttcagagt gaggacgctc ttgagggctc cggtattgcg gcgccacatc
     2761 cggacttcat ggccctgctc ggtcaggtcg gccgccgcgg cgtatgcgcc gttgccgccg
     2821 ccgataacgg agattttcat gggcgaaggc ttctattcct ttatcccgac ccagcccttg
     2881 tgggagatgt cgaacacgac gccgttcgga tcgcggaact tgcgctcgaa gtcgacgccg
     2941 ccgccgccct tcagctcgcg gtggaagcgc ccgccgttct gctgcacgcg cttcgagtag
     3001 ccctcgaggt cgtcgacgat gaagcccatg tggtggatgc cgtggaagtc cttgccgcgc
     3061 tcgtcgcccg ccgcgtcgtc ggaatggaac ttgagaagcg tcaggttgat gacgccgtcg
     3121 ctgagcgaga gcccgtcgcc gtagggcgtc tcgacgtagt tcacctcctc gaggccaaag
     3181 gtcttcttgt agaactccgc cgcggtcttc agctccggga ccgagatggc gatgtggcgc
     3241 agcttcgctg tcatagagac tccagtagcg cgtagaggtt ggccttgcgc tcgtagccgg
     3301 cgccgggcgc gtccggaatt gtgacgcgcc cgccggcgac gcgcacccca tcccaatagc
     3361 cgccgtacgg cagcgaggtg tcgggcgccg cctcggccat gccgaggccg aggccggcga
     3421 cgcaatgcgc cgcgaacagg tgcccagcgt gcggcaggaa ctgctttcgc ttccatcccg
     3481 ccgcctcgta ggcagcgagg atcttcgaat actccgtcac gccgtaggcg aggagcgggt
     3541 cgacctgcac gaagtcgcgc tcggggcgca ggccgccgta tcgcaggagg ttcctcgcgt
     3601 cgtcgaaaga gaaaaggttc tcgcctgtgg cgagcggcgt ggcgctcgag cgcgcgcacg
     3661 ccttgaggtg ctcgaagtcg agcggcggcg ccggctcctc gatccatgcc agcttgaaag
     3721 gctcgagcgc gtccagccat gggttcatct ttttctcttc aagagctcca ttgacgtcga
     3781 cagcccagat cgcgtttcgc ggcagcgacg ccgcggcgcg ctccaggcgc ttgaggtcgg
     3841 attcgacctc gcccgcgacc ttgatcttga cgcaggtata gccggcatcg atggcgttct
     3901 tcacctcgaa ggtgaggacg tcgtcggtgc ggaagtggcc gcaggaggcg tacacggcga
     3961 tctgcgattg agcttgcctt ccaaaatatt gcgcgatcga ctgccaaagc ggcaggtcct
     4021 gggccttcgc ccggaggtcc caggccgccg cctcgatcag gccgatcgcg ccggggcgct
     4081 cgccgtgccc gccgggcttc tcgtcgatca tggcggcgcg cgcgcaggcc gccgggtcga
     4141 tgagccccgc ggcatcgagc agcgcctcgg gcttcgcgcg caacaggcgc ggaaggaagc
     4201 gctcgcgcag cagcgccccc ttgccgtagc ggccgatcga atcgaaggcg tagccgacga
     4261 gccctcggtc cgagacgagc gcgagcgcgg aggcggtcat cgcgccgaag ccgatcgagg
     4321 cattgcgcat gtcggcgccg atgccgacgg tgcgctcgcg ggcgtcagcg atgcgcacta
     4381 gaacggaacg gcgattgcgc gctgcggcgc ttcgctgtcg atgaggtcga cgcgcttgag
     4441 cacgatgcgc aggccccgcg cttcgcgccg caggcggtgc agcacgcgag ccgcgaacca
     4501 gcgcgcgtcg ccggcgcgcc actcggcgac gaccagcgcc gagcgcacct cgagatcgcc
     4561 ggaaacctcc actgcgctca cgtggtgcac ggtgcgcgag gctggcgact gcacgtgcat
     4621 gtcgggccga tcgagccgcg ccacgcgcat cgcgagcagc ggcttaggct cgtagaagag
     4681 cgacaacgcc tcctgcggcg acgcctgccg ggggttggtc ggcacccagt agatgccgtc
     4741 ctcggcgaag agcgcgagcc agtccgagaa gcgccgctcg tcgagcaggc gcgcctctgc
     4801 caggacgaac gcctcgagct cgtccttcat cgcgtcatgt attgcagcca ggtcgcaaac
     4861 tggtggcgga tggcgagctc gctggtgccg ctgccgagcg tcgaggcgtc gggctggcgg
     4921 cggtcggcga gggcaccgcg gcgcacgtcg atccagtcgc tcgccgcgct ttgcaggccg
     4981 cgctggcagc gctcgaaaat ctcgaggtcg tcggaagcca cgagcgacgc gggcgaagcc
     5041 gcgaggttga cgaactgcag cgtgagatcg tgcatcgccg cgtccgcgcc ctcgagccga
     5101 aagcactgca cttcgaccat cgtgcggtcg gcggcgagcg gctggacgac gcgcagcgcg
     5161 gcaaagcgcg agttcaccga gaccgtcggc cagaccaggt tgttgaagcg atcgaccgcc
     5221 agcaccgccg cggccttctc cgcgccatgg cgctcgacga gctgcgcgcg gtagcgctcg
     5281 tatacgggat cggcgcgctc cggggcgatg acgccgctgc ggtagaagcc tcccatgtag
     5341 acatgcccgc cgggatacgc gtgcagaggg accttgtccc actcgggcgc gcgcatgccg
     5401 ttggcgagga acatctgcgc gccctgctcc gtaagcgggt ctgcaggctg cgagcgagcc
     5461 gctccaagcg cttggcgcgc cccttctatg gagctgcggt gcacgaagtt cggatgcacc
     5521 aggtcgaccg cgttctccat gaacatcttc cagttgccgc ggtactccag ccgcagctcg
     5581 ccgccgaagc gcgacaccgt gcccgccggc gcgcggtcga gcatgttgtc gaacgcgctc
     5641 gccaggccgc cgaggaactc ggcgagcccc ggaccctgcg cggcatgcga gccgaagacg
     5701 aagccgcgat aactttcgac gcgcgccacc cgcgcgaggc cgagctcgac cttttccgca
     5761 tagccctcgg ggagcggcat gccgaccagg cggccgtcga gcgcgtaggt ccaggagtga
     5821 taggggcagc ggaactggcg cgcgttgccc gccggctggg cgacgatgcg cgcgccgcgg
     5881 tgggcacagc ggttgtgcag cagcgcgagg ctgcggtcct cgccgcgcac gagcagcacc
     5941 tcgtccggcc cgaggcgccg caggacgtag tcgccgggct gcggcacctc gctctcatgc
     6001 ccgacgtaga gccaagcgcg ccggaaaatg cgttcctgct cgagcgcgaa cacctccgga
     6061 tcgacgtagg cggcgcggtg cacgctgttc gcgcgccaga aatcggcgac gttcatccgc
     6121 tagaacggca ccgcgaagcg caggtgcccg ctgtcctggt cggcgttcag caggtcgacg
     6181 cgcttggcgc cgatcaggaa gctctcgccc tgcggccgca ggcgccatga cgcccggccg
     6241 gcgaaccagc gctgctcctc gcggcgatgc tcgatgaaga gcaggtacgc ccgggcggcc
     6301 aactcttcgc cctcgccctc ttccacaaca acattactaa gatggtgatt gacccgggcc
     6361 atcggcgagt cgagcggcga cagctcgcgc tcgaggcggt cgacccgcat gcgcagcagc
     6421 gtcagcgtct cgtagaagag cgaaacatgg tccttcgggc tcttctgtcc ccaggtgtag
     6481 ggcacccagt actccgcgtc ctcggcgtac agcgcgagcc actcctgcag ccgccgctcg
     6541 tcgagcaggc gggcttcgtg gaacaagaat tgctgcacgc ggtgccagcg ctcgaggctc
     6601 atcccatgta ttgcgcccat gcgcggtgct ggttgcgcat cggcagctcg ttggtgccga
     6661 ccgcggcttc ttccgcatcc aatccgcgcg acaggtcgac ccattcgtgc tgcccgccgg
     6721 cgagcgagcg ctggatgcgc tcgaagatct cgtggtcgtc gctggtgacg atcgaggacg
     6781 gcgaattgtt ggtgttcacc atgcgcaccg acgcctcgaa catctcgtcg ggcgcaccct
     6841 tcaggcggaa gatgtagccg tgctgctcgg tgcagtcgac attcacgggc tgcagcacgc
     6901 ggatctgcgc cacgcgcgaa ttcacgaaca ggttcgggta gacgatcgtg ttgtggcggt
     6961 tgaacgagag aatctcggcg gcgcgctttt cgccgtggcg ctcgaccatg attttccggt
     7021 agcgcgcctg gagcgggttc tcatcgatcg cgcgggtgag gaagcccgcc atccagcaat
     7081 gaccgcgcgg catggcggcg atctgcatgc cgtcccactc gcgcagcgtg aggccgttcg
     7141 acttgacgat gtcccactcg atcgagggcg gcccgccacc gtggtcgacg cgatcggcgg
     7201 cgtcgatcgc gctgcggtgc aggatgcgcg ggtgcagcag gtcgatcgag ttctcgagct
     7261 gcagcttcca gttgccgcgg tagcgctgca cggttttccc gcccgcgagc tcgacctcgc
     7321 cgtcgggcgc gcgctcgacc aggttgtcga aggcggcgcg cacttccggc ccgaggtagg
     7381 tctccaagtc gtcgccctcg gcggcatggc tgccgaatat gaagccgcgg taggacgcga
     7441 cgcgcgccac cggctccagg cgcaatccgg ccatgcggct gcggaactcc gcgccatagc
     7501 cctgctcgag cggcaccgaa gcgagcgagc cgtcggtgcg ataggtccac gcgtggtagg
     7561 ggcacacgaa actgcgcgca ttgcccgcgg ctgccaggca gacctcggcg ccgcgatgcg
     7621 tgcagcggtt atggaaggca tggatcgtgc cgtcggtgtg gcgcgccacg atgacgcgct
     7681 cgcccgcgag gcgcgcggtg aagaagtctc ccggccgctt gagctggctc tcgtggccga
     7741 gccacagcca gacgcgccgg aaaatacgct cgcgctcctc ctcgaatacc gccgggtcgg
     7801 tgtagaagcg ccggtgcacc cgcccacgcc cgacgagctc cctgtattcc tcgcgcatgc
     7861 gcccatgctt atcctacaat ccgccgggat gcacaaggtt tcgatccggc cgcatatcgt
     7921 cgagcgcgtg gtcgcgcgcc gcggcgacct gcactggttc actcggctcg acgccgcaaa
     7981 gaccgcgctg ctggtgatcg acatgcagaa caccttctgc atgcccggcg cgccgggcga
     8041 ggtgccggcg gcgcgcgcca tcgtaccgaa catcaaccga ttcgccgcgg cactgcgcgc
     8101 gcacggcgtg ccggtggtct ggatcctgca cgccaacacg gtgcagaacg gccacagcga
     8161 ctgggaggtg ttcttcggac acgtggtgcg caacgcggac gtgcgccgtc gaatggccga
     8221 gagcctcgcg cccgcgcgcc aggcggtgtg gaaggacctc gaggtcgcgc cgggcgacgt
     8281 gaccctggtg aagaaccgct acagcgcgct cgcgcacggc gcctcgacgc tcgagcgcgt
     8341 gctgcgcaac ctcggcgtcg acaccgtgct ggtagccggc accaagacca acgtgtgctg
     8401 cgattccacc gcgcgcgacg ccatgatgct cgacttcaag tcgatcatgg tgtcggactg
     8461 ctgcgccgcg ctttccgacg aagagcacct ggcgtcgctc gagatcttca tccagcagtt
     8521 cggcgacgtg atgaccgccg acgaggcgct ggaacgcctt gccaaaacct agagggggac
     8581 ccgaatgaag gcattcaagg caacactctt tgcactgctc gccgcggccg gcaccgcaag
     8641 cgcccaggaa acgacgctca acgcggtggt gttcgtgccg cgcaacacca ccttcggcga
     8701 gattttcgtg cgcttcgtcg accaggtgaa caaggaaggc aagggcctgg tgcaggtcaa
     8761 gctgatcggc gggcccgacg cgatcccgac cttcgagcag ggcagcgcgc tgcgcaccgg
     8821 cgtggtcgac atggtgtcgc tgccgccgac tttctatacg aacgtttgcc cggagtgcga
     8881 cgcgcagatc ctgccgccgc tgcagacccc gcagctcaag aagacgccgt tctggaatgt
     8941 gctgcagaaa tacaccaacc agaaggtcaa cgcgcagctc ctgaccgcct acggcgacgg
     9001 catcggcttc cacatctgga cgagcaagcc accggccggc ggcacgttga agggcatgcg
     9061 cctgcgcacc acgccgaact acaccccgct cttcaccgca ctgggcgtgt cgctggtgca
     9121 gacggcgccc ggcgaagtcc tcaccgcgct cgagcgcggc gtggttgacg gctacggctg
     9181 gccggcgatc gggatcttcg acctcggctg gggtgcgcac accaagtacc gcatcgaccc
     9241 ggggtcctac aacgtgatcg tgaacgtgct ggtgaacctc aacaagtgga acgcgctgcg
     9301 ccccgaccag cgcgccttcc tcaaccgcat gggcgactgg ctggaggccg agaacgtgcg
     9361 ctgggtcgcc gagcgcaagg ccgccgacga caagaagatc aaggaggccg gcatccagct
     9421 ggtcgacctc gggccggcgc accgccagct cgcgtacgac gcgtactggg acacgctcgc
     9481 caagcgcgcg cccgagccga tcaaggaact gcgcgccgcc tccgacgccc tgcgttgagg
     9541 cgggtactcg aggatctggt catccccgcg ggacggggac acgctttcct cgtcccgcgc
     9601 ggcgccgtgc tgcgcatcca cctcgtcgag gaccggcagg tgggcgactg ctgcttcttc
     9661 aacgccaacg atcgccgcga ggtattccac gtcggccaga gctgggcgct gaatgtcatg
     9721 ctcggcaccg gcaacgcgaa gagcttccgg cacttctatt ccaagccgcc gcgcgagaac
     9781 gtgatgctca ccgtgctcga ggacacggtg cgcaaccact ggggcaacat gggcgggcgc
     9841 tgctcgaagc ggctctacga attgcgcgac ggcgaccgcg accaccgctc ctgccaggag
     9901 aacctcgccg aggcgctcgc gccctacggc gtctgcggcg acgacatcgt cgacatcttc
     9961 aacgtcttca tgaacgtcga gatgcgcccc gacggatcct tcacgatcct gccgccgagc
    10021 gcgaagaagg gcgactacat cgacctgcgc gcggaaatgg acgtgctcgc cgcggtctcg
    10081 gcctgtccgg cggatcgcaa tgccaccaac gacggccgcg ccaaacccct cggcattacc
    10141 atttcaacct gagaacagct ccacgacctg ctggcgcagc cagcgattgc ccggatcggc
    10201 ttcgaagcgc tcgtgccagt gcaccgcgac ctccgccgac gggatcggca ccggcggtgg
    10261 cagcgctttg agctttccgc ggcgctcgaa gacgcgcgcc acgcgcgccg gcagcgtcag
    10321 aatcaggtcg cttcgctcga gcaccatcgg cacgaccgtg aagtgaggca cgcgcagcgc
    10381 gatgcggcgc gccacgccgg cgcgctcgag cgcctcctcg atcacgcggt ggccgccgcg
    10441 atagatgacc agcgcgtgcg aggcgtcgag gaatcgcttt ttcgtgagcg tggtgatcgg
    10501 gtggtcggcg cgcatcaggc ataggtaggg atcgcggaac agcgcctggc gccgcaccgg
    10561 cgcggcaagg cccggcagga aaccgatcgc gaggtcgagc gctccggcgg ccagcgcgtc
    10621 ggcgatgtcc tcgacttcca gcgccaccgc ttccaggcgt gcgccgggag cgtcgcgctg
    10681 gacccgctcc accagcggcg gcaggaactc gatctgaccg agatccgaca tgtagaagcg
    10741 aaaagcgcgc gtcgaggttg cggggtcgaa gccggggccg tgcgcgagcg ccgattcgag
    10801 cagggccaga gcctggcgca ccggctcggc gagctcgcgg gcgaacggcg tggcgtcgac
    10861 gccgcgcggc gtgcgcacga aaagcgcgtc gccgaagacc ttgcgcaggc gcgccagcgc
    10921 gttggacacg gcgggctgcg tcagccccag cctcgccgcg gccggcgtca cgcccttttc
    10981 atgcagcacc gcatcgaaca cccgcagcag gttgaggtcg atgtcgtgga cattcatatc
    11041 agtgatcaat aacatgaatg acattgattt gacaaatgga aagccggacg cctaatctgt
    11101 ccggttccta gaggaggaat tcatgcgtaa gagcgttttg ctggccctgc tgctcgcgcc
    11161 ggcggtcgtg gcggcgcagg agaccgcgtt gcgcgtggtg agcgcgttcg ccgagaacac
    11221 ccagtacgtg aagaacctga tgccgatgat ccagaacctg aacaccgagg gcaagggttc
    11281 gctgcagctc cacttcatcg gcgggccgaa ggccatgccg ccgttcgagg tcggcaacgc
    11341 cgtgcgcacc ggcgtggtcg acgtggcgat gaccaccggc gcgttctaca ccaacatcat
    11401 gccggaggcc gacgcgctca agctcacgca gatgccgcac agcgagctgc gcaagaacgg
    11461 gggccacgac ctgatcaacc ggatctggaa cgagaaggcc aacatgcagt acctcggccg
    11521 ggtgatcgac tacacgccgt tccacctgta tctcaccaag aagatcgaca agcccgacct
    11581 caccgggctc aagctgcgca taacgccggt gtaccgcgac ttcttccagg cgctcggcgc
    11641 gaccgtgatg cagaccgcgc ccggcgaggt gtataccgcg ctcgagcggg gcgtggtcga
    11701 cggctacggc tggccgatca acggcatttt cgacttcaac tggcacgaga agaccaagtt
    11761 ccgcgtcgac ccgggcttct acagcgcgga agtgtcgctg gtcatgaacc tcgacaagtg
    11821 gaaagccttg agcgccgcgc agcgcgagct gctcaccaag cacgtgatcg cgctcgaggc
    11881 cggcaacgac tcctggaaga aggtgaacga ggacgacatc cgcaagcaga aggaagccgg
    11941 gattcaagtc atcaccttcg acgcggcgac tgccaagcag tactacgacc gggcctacga
    12001 ggtcgcctgg accaacgcga tcaaggcgag cccgcagtac gggccgcaga tgcggaagct
    12061 gttcagcaag tgatctggta tcgaaaattg ctgcatgggc tggctggcgc ggccgcgctg
    12121 ctgctcggcg ccatggcgct gctcgtcacg ctcgacgtgc tgatgcgcaa cgtcggcgtg
    12181 ggcacgctcg cctgggtgaa cgaggtgtcc gagtacagcc tgccgctcgc cacgctgctg
    12241 atcgcgccgt ggctgctcca ccgcaacgag cacgtgcggc tggatgtgct gctggtttcg
    12301 ctgccccggg cggcgtcgcg cctgctcgag aaggcctgtg acgtcgtcgg catcgcgatt
    12361 tgcgcagtgt tcgtctggta cagcctgcgg ctgatcctcg actcctcgcg cattggctcg
    12421 atggtggtga agacgctctc gatccccgag tggtggcagt acgcgcttgt gccggtgtgc
    12481 ttctctcttc tcgccgtgga gttcgcgcgg cgactgcggg catgacatgg tggctgtccg
    12541 caatcgtgct ctttggtgtg ctgacggcgc tctttctcgt cgggctgccg gccgcgttcg
    12601 cgttcttcgg catcaacatc gtcggcgcct ggttctacct gggcaaggac gccggcctgg
    12661 tgcagctcat ccgcaatggc atcgtctcga tcaccagctt ctcgctcacg ccgatcccgt
    12721 tcttcatatt gatgggggaa gtgctgttcc acaccggcgt agcgatgaag gcgatcgacg
    12781 cgatcgaccg ggtgatctgg cgcgtgccgg gacgtctgtc ggtggtggca gtggtcgccg
    12841 gcaccgtgtt ctcggcgatt tcagggtcga cgatcgcgac gaccgcgctc ctcggcagcc
    12901 tgctgctgcc gcagatgctc cggcgcggct atcacccgaa tatggccatg gggccgatca
    12961 tggcgatcgg cggcgtggac atgctgatcc cgccctcggc gctgaccgtg ctgctcggca
    13021 gcctcgcggg aatctcgatc tcgcagctgc tgatcgccgg catcgtgccg ggcgtcctgc
    13081 tgtcgctgat gttcatcggc tggatcgtgc tgcgcgccgt ggccgacccg tcgctcgcgc
    13141 catccttcga gcacgaagag ctgccgctct gggagcggct gagacccctg ctgctctacg
    13201 tgatgccgct ggtgctgatc ttcgtcgtcg tggtggcggc gatgaccgca ggctgggcga
    13261 ccccgaccga agccgccgcc atcggcgcgg tggcgaccgt ggccgtggcc tggatctatc
    13321 gcgcgctcac ctggcgcaac ctgatgcagg cgctgctcgg caccgccggc atctcaggaa
    13381 tcatcctgtt catcatcatc ggcgccacga ccttcgccca gatcctgacc ttttccggcg
    13441 cgaccaacgg catggtcgaa gcgatccgcg atgcgggctt cgcctcctgg acggtgctga
    13501 tcgccatgct gctggtgctg ctgctcctcg gctgcttcgt cgaccaggtc agcatgatgc
    13561 tcatctgcct gccgttcttc atgccgctgg tggcgctcta cggcttcgac ccggtgtggt
    13621 tcggggtgct gttcctgatc tgcatgcagc ttggcctgct gacgccgccc ttcggcatgc
    13681 tgctcttcac catgaagagc gtggcgccgg cggggattga gatgcgccag gtctggcacg
    13741 ccgtcacccc ctacgtcgtc atgggcgtct tcgcgctcgt cgcggtgatg atgtttccgc
    13801 cgctcgcgac ctggctgccc aagctgatgt tcaaatagcc atggaaagat cgttcaagga
    13861 agaagtcgag aagctcaggc tgggcgaagg cgagaccttc cacggcgagg gcatcctcgc
    13921 cgtgaccaag gcgctgctgc agtcgggagt gtcctacgtc ggcggctacc agggcgcccc
    13981 ggtgtcgcac gtcatggacg tgctgaacga cgcgcgcgac atcctcgacg agctcggcgt
    14041 gcacgtcgag gcgaacgcca acgaggccgg cgccgcggcg atgctaggcg cctcgatcaa
    14101 ctacccgatg cgcggcgcgg tcgccttcaa gtcgaccgtc ggcaccaacg tcgcctcgga
    14161 tgcgctttcc aacctcgcgt ccgccggcgt aaagggcggc gcgctcatcg tgctcggcga
    14221 ggactacggc gaaggcgcct cgatcatcca ggagcgcagc cacgccttcg ccatgaagtc
    14281 gcagatctgg ctgctcgacc cgcgccccaa cctgccgaag atcgtcgaga tggtcgagca
    14341 gggcttcgag ctgtcggaag cgtcgagcac gccggtgatg ctcgagctgc gcatccgcgc
    14401 ctgccacgtg cagggcagct tcaagacccg caagaaccag gcgccgcaat tctcgaagaa
    14461 ggcattcctc cagaacccgg acttcgacta cggcaagatc tgcctgccgc ccgcgaccta
    14521 cgcgcaggag aagcagaagg tcgacgtgcg ctggccggcg gcagtccggt tcatccgcga
    14581 acgcaagttg aatgaatttt ttgatggaga acaaagcgat ctcggcatcc tttgccaggg
    14641 tggcatgtat aacgcagtga tccgcgcgct gcagatgctc ggtctcgccg acgccttcga
    14701 cgcctcgcgc gtgccgatct actgcatgaa cgtgacctac cccctgatcc cggaagagat
    14761 cacggagttc tgcaaagaca agaaatcggt cctcatcgtc gaagaagacc agcccgccta
    14821 catcgaggac gcagtgctcg cggtgctgcg cagggccggg gccaatagcg tcaaggtcag
    14881 cggcaaggac atgctgccgc tcgccggtga atacaccggc gaagtggtgc tgaccggcgt
    14941 ctcaggattc ctcgatcgct cggagcgcgt tcagccgatt ctgtcgctga agaagaaagc
    15001 ttccgagctc ctcgccggcc tgccggcgcg cccgcccggg ttctgcgtcg gctgcccgga
    15061 acggccggtg ttctcggcga tgaaggtgct cgagaaggaa agcggcaaat tccacttgtc
    15121 ggcggacatt ggctgccatc tcttctcgac gctgccgccg ttcaacatcg gcaacacggt
    15181 gctcggctat gggctcgggc tcgcctcgaa ttccgcggtg cacccgatgt tcggcaagcg
    15241 caccgtgacc atgatgggcg acggcggctt ctggcataac ggcctgacct cggggatcgc
    15301 caacacggtg ttcaacaaga acgacggcat cctggtgatc atgaagaacg gctacagctc
    15361 cgccaccggg acgcaggaat tgccctcctc gccgcagcac agcgagcaca aggcgcccga
    15421 catgagcatg gagcgcgcgc tggaaggcat cggcgtcggc tgggtgaaga ccgtcgacaa
    15481 ctatcgcgtc ggcgaggtgg cgaagacgct caaggaagcc atgaccaccg actaccctgg
    15541 cctcaaggtg atcatcgccg agggtgagtg ccagctcgag cgccagcgca agcttcggcc
    15601 tttgacttca aaaagacttt ctgaaggaaa gcggaccgta cgcgtcaaat acggggtcga
    15661 cgaggatacc tgcaccggcg accactcgtg catccgcctc tcgggctgcc cgacgctcac
    15721 catcaaggac aaccccgatc cgctgcgccg cgatccggtc gccaccgtga tcgacggctg
    15781 cgtcggctgc ggcttgtgcg gcgagaacgc gcacgccgcc gtgctctgcc cctcgttcta
    15841 tcgagcagaa gttattcaaa accccaattg gtgggacaag ctttggaacg ccagttcact
    15901 aaggaaatag agtccctgcg gctcggggac ggcgagacct tccacggcga aggcatcctc
    15961 gccgtcacca aggcgctgct ccagtccggg gtttcgtacg tcggcggcta ccagggcgcc
    16021 ccggtatcgc acctgctcga cgtaatggtg caggcgaagg actatctcga cgagctcggc
    16081 gtgcacgtcg aggcctgcac cagcgaggcc tcggcggccg cgatgctcgg cgcctcgatc
    16141 aactacccgg cgcgcggcgc ggtgacgtgg aagtcgatcg tcggcaccaa cgtggccgcg
    16201 gacgcgcttt cctatctcgc gagcgcggga gtgatgggcg gcacactgat cgtgctcggc
    16261 gaggactacg gcgaaggcgg cgtggtagcg caggagcgca gccacgcctt cgcgctcaag
    16321 tcgctgatgt ggctcctcga cccgcggcca aatctcacca ccatcgtcga catggtcgag
    16381 cgcggattcg agctctccga ggtgtcgaac acgcccgtga tgatggagtt gcggatacgc
    16441 gcctgccacg ttcgcggttc tttcaagacc cgcaggaacc ttgcgcccga catctccacg
    16501 acgcaaaagc tcgacggtcc tccgaaagag ttcatctacg accgcctggc ccatccgccc
    16561 gcgaccttcc gccaggagaa gctcaagtcc gacgtcaggc tgccggctgc acgcaaattc
    16621 atcctggaaa agcggctgaa cgaaatcttc cccggcggcg aaaacgtcgg cgtgatcgtg
    16681 cagggcggtc tgttcaacgg cctgatgctg gcattgcgca cgctcgggct ggccgaccag
    16741 tttggcgcca gccgcattcc gatcctcgcg ctgaacgtgg tgtatccgct ggtgccggag
    16801 gaaatcaacg gcttttgcga ggacaagaag gccgtgctgg tcctggaaga aggacagccc
    16861 gaattcatcg agcaggaggt cgcgactttg ctacggcgcg cccgtgtcgg cgcggaattg
    16921 cacggcaagg actgcgtgca catggcgggc gagtacaacg tcgaggcgtt ggtgcggggg
    16981 ctcgcccgtt tcctcggcat gcacgctgcg catctggaca cttccgcagg ctcggcctgg
    17041 ctggactcgg tccagggtac gaagaagaaa gccacagccc tgctcggcgc gctgccggcc
    17101 cgcccgccga cgttctgcgt cggctgcccg gagcgcccgg tgttctcggc gatgaagctg
    17161 gtggcgcagg acatcggccg gccgcacgtg tcgatggacg tgggctgcca cgc
//
LOCUS       DATBSZ010000166        19612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_982795, whole genome shotgun sequence.
ACCESSION   DATBSZ010000166 DATBSZ010000000
VERSION     DATBSZ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19612
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982795"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VIG70_10965"
     CDS             complement(<1..1066)
                     /locus_tag="VIG70_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8251120.1"
                     /translation="MERVTTQQLAMMPISFKLNGKTVIGRPDETLIECASKNGVEIPH
                     LCYKPGLRPDGNCRACVVEIKGERALAPSCCRFPKEGMEVTTDNERAVLSQKMVLELL
                     LSDMPERDYTRNNELDQWAQKMKMGKPRFESRKQVQADLSHPAIAVNLDACIQCTRCL
                     RACREEQVNDVIGYAHRGEHSKIVFDFDDPMGQSTCVACGECVQACPTGALMPARDVG
                     LQVIDKRVDSVCPFCGVGCLLTYHVNTKQNVIKFVNGKDGPSNHNRLCVKGRYGFDYA
                     HHPHRLTRPLIRRPGVEKSADFVVDPGNWSEVFREASWEEALELAAGGLKKIRDQDRY
                     ALAGFGSAKGSNEEAYLFQKL"
     gene            complement(1066..2727)
                     /locus_tag="VIG70_10970"
     CDS             complement(1066..2727)
                     /locus_tag="VIG70_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY8251121.1"
                     /translation="MRGKGKVRSTPKGRSVDPQALEQVRALLGSESRQRDLLIEHLHK
                     VNDRYGHLSAAHLAALAQEMKLSLTEVYEVATFYHHFEVVKEGEAPPAALTVRVCDSI
                     ACELAGSENLLSKLPSLLGKEVRVLHAPCVGRCEVAPCVVVGQNPIGNATPETVRNAV
                     SSRNTACPETRYVDFAAYKKQGGYKLIADCLAGKRTREELTKIMEDSGLRGLGGAGFP
                     AGRKWRLVSAEPEPRLMALNIDEGEPGTFKDRWYLERDPHRFLEGMLVAAWCAGVGEI
                     YVYLRDEYAGCRAILERELKKLQTGAPFPLPPIHLRRGAGAYICGEESAMIESIEGKR
                     GMPRLRPPYVAQVGLFGRPTLEHNMETVHWVREIVEKGPQWFASQGRNGRKGPRSFSV
                     SGRVKKPGVHLAPAGITVLQLIEEYCGGMLDGHEFYGYLPGGASGGILPASKGDIPLD
                     FDTLQPHGCFIGSAAIVIFSHKDKAAKAAVNLMKFFHDESCGQCTPCRVGTAKAVTLM
                     EARKWDQPLLKELSQAMMDASICGLGQAAPNPALTVMKYFPHEVG"
     gene            2810..4735
                     /locus_tag="VIG70_10975"
     CDS             2810..4735
                     /locus_tag="VIG70_10975"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8251122.1"
                     /translation="MQAAWDALERNEYRVAEKAAREALARSPADAEALYLLGSTLLFE
                     GRSREALGPLSAAFDKLERRGVAYRLGYCHLALGDFARAEQALQRETQAYPDYANAHN
                     ALGVALVNQGRNEEALAAFVAATRLDPGHAEASNNAGSVLRTLDRPEDALPFLQRAVG
                     AQPGLADAHQNLGLVLHALRRYEEAVASFRQVIALAPRTPYALGSLVWAELFACDWRE
                     IGPHIAALREQVRTGEVAAAPFTLLATSDSAAEQRRCAELHVRETLGGTREPLWRGAR
                     YEHARIRLAYLSADFHEHATAYLAARLFELHDRSRFELIAISYGPDDRSATRARLQRA
                     FDKFVDVRAMSDAQAARLIHEMEVDIAIDLKGLTADSRITILAHRPAPVQATYLGYPG
                     TVGADYIDYVLADRVVIPPSDERFYTEKVVCLPDCYQINDATAAIAERTPSRAGAGLP
                     ERGFVFCCFNNTYKLGPGMFEIWMRLLAQEPHSVLWLLEHEALARRNLQRSAEARGID
                     AARLVFAPRVAHAEHLARHRLADLVLDTLPYNAHTTASDALWAGVPVVTCLGATFAGR
                     VGASLLNAVGLPELITRDLSAYEALALKLAKDRNFLSAIREKLGRNRLTHPLFDSART
                     CRHIEAAYERMRDRLGR"
     gene            4732..5697
                     /locus_tag="VIG70_10980"
     CDS             4732..5697
                     /locus_tag="VIG70_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8251123.1"
                     /translation="MKYRVLGRTGVQVSQLCCGTMAFGGDADEKEAARIYRAAREAGI
                     NFFDTADQYNKGRSEEVLGELIRGERDSLVLATKCFNPTGDDVNARGASRRHVSRAVE
                     ASLKRLGTDRVEILFLHHYDALTPLEEHMRGLEDVVRAGKALYTAVSNYAAWQTQAAL
                     GIQERAGWARLQAIQPMYSLLKRQAEVELLPMAAANGLSVFPYSPAAGGLLSGRYSEP
                     GAQGRHTTNKMYAVRYGEAWAQDAAAKFAALCRQRNLHPMSAAVAWVAAHPAVTAPII
                     GARSAEQLRASLDSVKVDMTPELRAEITALSRTPPPATDRLEELK"
     gene            5694..6020
                     /locus_tag="VIG70_10985"
     CDS             5694..6020
                     /locus_tag="VIG70_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251124.1"
                     /translation="MIGWFDCTAVDEFADAVVADLLKRVPPPSIESVGKVTAKRLKST
                     NDMIFARAESFARAQPLNVYKKAHLGNRVKWALREAGYAEEFVDALTYELVTVITLAA
                     GARKKP"
     gene            6017..6865
                     /locus_tag="VIG70_10990"
     CDS             6017..6865
                     /locus_tag="VIG70_10990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8251125.1"
                     /translation="MSRLARLAFDLRRERQAIPDAFPNYARDPVALATDRRLKGWARI
                     AGFFGVGLDLLAALCGTDKYGVHHYTPVYEALARPLRRQPVRLLELGVGGYGHGLGGE
                     SLLMWAAYFRRGRIYGLDIEDKTALSRGRIKVVQCSQTDRARLEALGAEIGPFDFVID
                     DGSHVNAHQIESFRILWPFVKDGGWYVVEDVQTSYWPHYGGGTPGSPRHASSCISFFK
                     SLADSVNECEFLERPDAEIVPSIGHIAFHHNLIVLRKDESRRTSNVPLGNEKVRAALM
                     KPRSNP"
     gene            complement(6909..7397)
                     /locus_tag="VIG70_10995"
     CDS             complement(6909..7397)
                     /locus_tag="VIG70_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8251126.1"
                     /translation="MKVLTHLLAADGSIPNHPRWPLLIYPQALKNAKPEAFEELFTRN
                     RWPAAWRNGVHPFHHFHSTSHEVLGIYEGEVTVQFGGEGGVTVTAKPGDVIVLPAGTG
                     HKKLAARGALGVVGAYPEGSNPDTCTTTTLKNVQAAIARVPMPAADPVYGVDGPLFSH
                     WR"
     gene            7420..8343
                     /locus_tag="VIG70_11000"
     CDS             7420..8343
                     /locus_tag="VIG70_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEY8251127.1"
                     /translation="MCRALLYLGEPVLLDNFLYQPDSALVRQSYMPKMLNLLNLAGFG
                     LRAWDPSSHDPDRAYSYYSQSLPVFDRNLKNLAEKIRTGCLLAHVRGVAYHTRVEISQ
                     QNCHPFHFSNVPLVLAHNGDLARFAEMKPLLSPFIKPVFLSQIHGTTDSEWVYALIVS
                     QLPDPHRHPSADELAKAVERALEIIRDARRKLGISISSSLNLFIADGTQLAAVRYCFD
                     FGRYPTADAERVHEANTSFLSLWYTAGHGYGLHDGEWKMTGGSDNATSVLVASEPLTR
                     DTAAWVEVPEYSMLHATIRKNRPAVTIRDLQ"
     gene            complement(8321..9352)
                     /locus_tag="VIG70_11005"
     CDS             complement(8321..9352)
                     /locus_tag="VIG70_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8251128.1"
                     /translation="MRTFLDRLDSEARDLLLAVARPITHAKGDRLVRYGDPSRGAYLL
                     KEGAAEATVLLPGGEKLTVATLGAGSVFGEMALFERGTCTATVTASEKLEGWFIERDD
                     FRSLVAQRVPAALRIQHAVTLALSDKLRALNAKVLEVAAPGDTPKSGTGHDADPLAKV
                     KRVKEASFDLKPFLSHLPFFEGFGPGETAEILEIAHVLELSRGHAVFNHGQPSTACFI
                     VVRGAVEIRAQHARRERRIAVLGPGQLLGYMSALEKGVHGSDAVVREQAVLLEIPGED
                     FEKLYFGSSPTSTRLHRVIQKSLLASLGQTNRHLTRLISQARLRGAEKESERLEKAYG
                     GQIIADLGS"
     gene            complement(9364..11019)
                     /locus_tag="VIG70_11010"
     CDS             complement(9364..11019)
                     /locus_tag="VIG70_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8251129.1"
                     /translation="MANQVYEKRREQMFPRLDARQIARLEPRGVRLRIRAGETLAEPG
                     DSHRKMLVVLSGKLEIFIPGARGEQSLTQLVPGDFAGELSTLRGLAGFSRIRVVEAGE
                     VLAVGEAELRTLVQTDAELSEIIMRAFILRRMGLVDSGESEVALVGSRHSANTLRLRE
                     FLTRNNHPYMSLDVDEDPGVEELLCRHGVGVDEVPVVLAPRGVLRNPDIRTLAGFLGM
                     NPEMDEAQVHDVVVVGAGPAGLAAAVYAASEGLDVMVIEALAYGGQAGSSSRIENYLG
                     FPTGISGGALAGRAFVQAQKFGAAVNVASHALRLDCSRRPYAIQLSDGHRALAKSVVV
                     ATGAKYRAPECTELQRFVGLGVYYAATHLEAKLCDGKEIAIVGGGNSAGQAAVFLANT
                     CRHVHMLVRGPGLAESMSRYLIRRIEENPAITLHTHTEIEEMEGGERLERVIWQRKGA
                     AAETREIGHVFLMTGASPNTAWLDGCLALDDKGFVRTGIELTAQDLAAAGWSLPRAPS
                     LLETSKPGVFAVGDVRSGSVKRVASAVGEGSSCVQFVHRVLGA"
     gene            complement(11032..11280)
                     /locus_tag="VIG70_11015"
     CDS             complement(11032..11280)
                     /locus_tag="VIG70_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8251130.1"
                     /translation="MASIRREFLIDARPTDVWEAVRDIGALHTRLVPGFVTACRLEPG
                     ARTRAVWTADLLPDEMAKPIAAMIDQGCAVTKKTLERG"
     gene            11310..14123
                     /locus_tag="VIG70_11020"
     CDS             11310..14123
                     /locus_tag="VIG70_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8251131.1"
                     /translation="MNQSIPQPAAEQIDPNRVLAVVAAVAREARPNVEVAVALDSSLE
                     RELGLDSLARVELVLRLEREFTTSLPEQALASSETPRDLLRFLLASHGHAPQTADRTV
                     ASLSQAEGVRPPDHAQTLTEALEYYVERQPDRLTVFLYEEQKETQITYRTLWEGSLAY
                     AAGLAASGLMPGQTVAIMLPTSKEYLFCFYGTLLAGGIPVPLYPPARLTTIEDHLTRH
                     VGILKSCRAALMVTIPEAKAIAWLLRAQVETLRAVMVPADFPASAKDFVPVKTTSGQT
                     GFLQYTSGSTGQPKGVVLTHANLLANVRAMGKAARATSADVFVSWLPLYHDMGLIGGC
                     FATMVLSFPVVLMSPLAFLARPSSWLRAIHRHGGTISGGPNFCYELCMRRIPEHELEG
                     VDLSTWRFAFNGAEPVSTETMTGFTEKFSRWGFRKNAMTPVYGLAEASVGLAFTPPGN
                     PWQVDLLDREHFSSTGEAVLAREDDPAPLKVVACGRPIPDHDLRVVDGAGFELPDRSE
                     GSLQFRGPSATTGYYRNPEATKALFDGEWVNTGDRAYLSDGTLYLTGREKDIIIRGGR
                     NISPYELEEAVGDIPGVRRGCVAVFGSLDPASGTERIVVLAETRQPGGAGEADIKRRI
                     NDLSMNLIGAPVDDIVLAPPHTVPKTSSGKIRRVAARDYYERGPESVKPQAVWLQFIR
                     LAAAGVAPQIRRGWRVARGILFALRAYAVFLITTPWIFGAAATRNVGLTWKVGGVMTR
                     LFLRLAGLRVLVRGLENIPPGPVVLTANHTSYFDALVLVSILGPRNFSFIAKREFLAN
                     PLMRLLLTGFGCVFVERFDVQAAAGHADELAEAAKRGQSLAIFPEGTLLRRPGLLPFR
                     TGAFQVAAQAGIPVVPVAMRGVRSVLRDGTWYARRHPMAVTFGAPIAPEGNDWNAAVR
                     LRDLVRAEVLKHCGEPDLAAENL"
     gene            complement(14120..15967)
                     /locus_tag="VIG70_11025"
     CDS             complement(14120..15967)
                     /locus_tag="VIG70_11025"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8251132.1"
                     /translation="MLKWLLGRGKAPLPAGAQDCFQLGSDLAQRGRLDEAIVLLRRAV
                     ELEPLNAAAHHNLGSALRDRGERPAALGAYREAARIEPAFAEAHYGIGLMLVEERAYE
                     EALASLLRAIKLSPRLAEARLQAGNAHMGLGNWQAALAEFKAAIEARPDYAEARWARA
                     VSHLPAVYGAGVDPAERRRAFAAELKSLRKWFAASKRPDAFLAVGVHQPFYLAYQESP
                     NRELLAEYGELCAGQMGRWQSRAGLALPAKRPRSKPPPGSGSKPRIGIVSAHIYEHSV
                     WMALVRGWVERLAERAELHVFHLGSGGDAETAFARKRAAQFHAGLRPFDQWAGLIHAA
                     GLDLVIYPEIGMDATTAKLASMRLAPVQAASWGHPETTGLPTIDYYLSAEGLEPPDAQ
                     ANYTEKLIALPNLGCSLSPGKEEAGRFSGLELRKDELLLVCPGTAFKYAPQHDRVLAE
                     IARRVPGSRLVFFRSRPEPLAEKLRERLRDSFRRAEVDFERQVTFIPWQPTGAFRALL
                     RRADLYLDTIGFSGFNTAVHALECGLPIVACEGRFLRGRLASGPLRRLGLDELVAPSA
                     EAYVELAAALAADAGRRQALRRRIESSRERLFRDAAPLDALEDFVRAVT"
     gene            complement(15961..16797)
                     /locus_tag="VIG70_11030"
     CDS             complement(15961..16797)
                     /locus_tag="VIG70_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY8251133.1"
                     /translation="MAQLRFKQVDVFTDRPFLGNPVAVVIGGEDLDAAAMQAIACWTN
                     LSETTFLLKSSKADYRLRIFTPKHELPFAGHPTIGSAHAALESGFVGKKKKLVQECGA
                     GLIELSVEDDGRIFLKGPPAKIQKMERQIPSIPLVPGGDVIKVDVGPVWVVGEMRDAR
                     ALADLKPDMTALAEWSLSLGVSGATVFAASGESASAIHVRSFAPAHSIPEDPVCGSGN
                     LSVAVFLKEQHLLERFGSTYVARQGMQLGRDGHVSVRIGEKIEIGGHAVTCVDGTLAP
                     PC"
     gene            complement(16876..18867)
                     /locus_tag="VIG70_11035"
     CDS             complement(16876..18867)
                     /locus_tag="VIG70_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY8251134.1"
                     /translation="MLATLPYAGTIFLSSFLLFLVQPIIAKQILPWFGGSAAVWTTCL
                     VFFQSVLLAGYAYADWTTRLGPRRQAALHVGLLAVSLLCLSILASTGWKPQGDEQPIL
                     RILLLLGATIGLPYFLLSTTTPLLQAWYWRRFRTVVPYRLFALSNLASLLALLGFPVL
                     FEPAFDLTSLGWGWSFLFAGFAILCAGTAWLSVNGFETEARTAATPVPLKNQLQWLAL
                     SAMGSVMLLAVTNHVTQNISSVPFLWVLPLALYLASFILAFDHPRWYIRPAFVGALVV
                     LVPLMAYAIPSLDLRLAAPLYLAGLFVACMVCHGELARLKPDPAHLTRFYLMISLGGA
                     LGAVLVAIVAPLVLAGYFELGIALVALGAVTILVKKIRWVGAAVTVVSALFVFRGAQE
                     YTEGVRVMERDFYGVVRTADHENPVPYRAMYHGAIMHGGQLLGDSFRNTPADYFSPGS
                     GYGRVFASLREMQPKKPLAVGVIGLGAGVIAAWMKPGDRLVFYEISPRVVDIARREFT
                     FLHDTSAKAEIILGDGRLSLEREPPRGYDVLGIDAFSGDSIPMHLVTREAMAIYLKHI
                     KPDGVIVFQATNRFIDLLPVVKRLAAEFGLEALNVSDVPEGEDGAEYWYSSTDQVIVT
                     RNPKLLGWPRIAEAAEEIADRPGLPVFTDAHHNLLRILK"
     gene            complement(18879..>19612)
                     /gene="sucD"
                     /locus_tag="VIG70_11040"
     CDS             complement(18879..>19612)
                     /gene="sucD"
                     /locus_tag="VIG70_11040"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY8251135.1"
                     /translation="NFEGIPIFGSVKDAKAQTGANVSVIYVPPPFAASAIDEAIDADL
                     DLVICITEGIPVRDMIRTKHRMQGTRTLLVGPNCPGVITPDQIKIGIMPGHIHKRGPV
                     GVVSRSGTLTYEAVGQLTELGLGQSSCVGIGGDPVNGLKHIDVLRMFNDDPKTEAVIM
                     VGEIGGSDEEAAARWVKANMKKPVVGFIAGVTAPPGKRMGHAGAIISGGKGTAQEKLA
                     VMEECGIKTTRNPAEMGRTLKSVLK"
BASE COUNT         3125 a         6795 c         6592 g         3100 t
ORIGIN      
        1 ccagcttctg gaagaggtag gcctcttcgt tcgatccctt ggccgagccg aagcccgcga
       61 gcgcgtagcg gtcctggtcg cggatcttct tcagcccgcc ggccgccagc tcgagcgcct
      121 cctcccagct cgcctcgcgg aacacctccg accagttgcc ggggtcgacg acgaagtccg
      181 ccgacttctc cacgccgggc ctgcggatca gcggccgggt caggcgatgc ggatggtgcg
      241 cgtagtcgaa gccgtagcgc cccttcacgc acaggcggtt gtggttcgac gggccgtcct
      301 tgccgttgac gaacttgatg acgttctgct tcgtgttgac gtgataggtc agcaggcagc
      361 cgacgccgca gaacgggcag accgagtcga cgcgcttgtc gatcacctgc aggcccacgt
      421 cgcgcgccgg catcagcgcg ccggtcgggc aggcctgcac gcactcgccg caggcgacgc
      481 acgtcgactg ccccatcgga tcgtcgaagt cgaacacgat cttcgagtgc tcgccgcggt
      541 gcgcgtaccc gatcacgtcg ttgacctgct cctcgcggca ggcgcgcagg cagcgcgtgc
      601 actggatgca ggcgtcgagg ttgaccgcga tcgcggggtg cgacaggtcc gcctgcacct
      661 gcttgcgcga ctcgaagcgg ggcttgccca tcttcatctt ctgcgcccac tggtcgagct
      721 cgttgttacg cgtgtagtcc cgctcgggca tgtccgacaa aagcagctcg agcaccatct
      781 tctgcgacag caccgcgcgc tcgttgtcgg tggtgacctc catcccctcc ttcgggaagc
      841 ggcagcagga gggcgcgagc gcgcgctcgc ccttgatctc gactacgcag gcgcggcagt
      901 tgccgtcggg gcgcaggccc ggcttgtagc agaggtgcgg gatctcgacg ccgttcttgc
      961 ttgcgcactc gatgagggtc tcgtccgggc ggccgatcac cgtcttgccg ttcagcttga
     1021 acgagatcgg catcatcgcg agctgctggg tcgtgacgcg ttccatcaac ctacctcgtg
     1081 cggaaaatat ttcatgaccg tcaatgcggg attgggcgcc gcctgcccga ggccgcagat
     1141 cgaagcgtcc atcatcgcct gagacagttc cttcagcaac ggctggtccc acttgcgcgc
     1201 ttccatgagc gtgaccgctt tcgccgtgcc caccctgcag ggggtgcact ggccgcagga
     1261 ctcgtcgtgg aagaacttca tcaggttcac ggctgccttt gcggccttgt ctttgtgact
     1321 gaagatcacg atcgcggccg acccgatgaa gcagccgtgc ggctgcagcg tgtcgaagtc
     1381 gagcgggata tcgcctttcg acgccggcag gatgccgccc gaagcgccgc cgggcagata
     1441 gccgtagaac tcgtgcccat cgagcatgcc gccgcaatac tcctcaatta gctgcaaaac
     1501 agtgattcct gcaggagcaa ggtggactcc cggcttcttc acccgccccg atacagagaa
     1561 agaccgcggg cccttgcgtc cgttccgccc ctgcgaggcg aaccactgcg gccccttctc
     1621 gacgatctcg cgcacccagt gcaccgtctc catgttgtgc tcgagcgtcg gccggccgaa
     1681 gaggcccacc tgcgcgacgt agggcgggcg caggcgcggc atgccgcgct tgccctcgat
     1741 cgactcgatc atcgccgact cttcgccgca gatgtacgcg cccgcgcctc ttctcagatg
     1801 tattgggggt aacgggaagg gcgcgccagt ttgcagtttc ttgagctccc tttcgagaat
     1861 tgcgcggcag ccggcgtact cgtcgcgcag gtacacgtaa atctcgccga cgccggcgca
     1921 ccacgcggcg accagcatgc cctcgaggaa gcggtgcgga tcgcgctcca ggtaccagcg
     1981 gtccttgaac gttcccggct cgccttcgtc gatgttgagc gccatgaggc gcggctcggg
     2041 ctcggccgac accaggcgcc acttgcgccc cgccgggaac ccggcgccgc cgaggccgcg
     2101 caggcccgag tcctccatga tcttggtgag ctcctcgcgc gtgcgcttgc cggcgaggca
     2161 gtcggcgatc agcttgtagc cgccctgctt cttgtacgcc gcgaagtcga cgtaccgggt
     2221 ttccgggcac gcggtgtttc gcgaagaaac ggcgtttcgc accgtttcag gcgtggcatt
     2281 tccgatcggg ttctggccca ccacgacgca gggcgcgact tcgcagcggc ccacgcatgg
     2341 ggcgtggagg acccgcactt cctttccgag caaagacggc aatttcgaaa ggagattctc
     2401 agaacctgcg agctcgcacg cgatcgagtc gcaaacccgc accgtgagcg cggccggcgg
     2461 cgcctcgcct tccttgacca cttcgaagtg gtgatagaag gtcgccacct cgtacacctc
     2521 ggtgagcgag agcttcatct cctgcgccag cgcggccagg tgcgcggccg aaagatgccc
     2581 gtagcggtcg ttcaccttgt gcaggtgctc gatcagcagg tcgcgctgcc gggattcgct
     2641 ccccagcagc gcgcgcacct gctcgagcgc ctgcggatcg accgaccggc ccttcggcgt
     2701 ggaccggact tttccttttc ctctcatcgc tctgcttatt atcgggcacg cggctgaggc
     2761 ccattcttga ctgcggtcaa gcacacggga tgcgagaatc gcccgcaaca tgcaggcagc
     2821 gtgggacgcg ctcgagcgca acgagtaccg cgtcgcagag aaggcggcgc gcgaggcgct
     2881 cgcacgctcg ccggccgacg ccgaggcgct ctatctcctc ggcagcacgc tgctgttcga
     2941 aggccgctcc cgggaagcgc tcggcccgct gagcgccgcg ttcgacaagc tagagcgccg
     3001 cggcgtcgcc taccggctgg ggtattgcca tctcgcgctt ggggatttcg cgcgcgcgga
     3061 gcaggcgctg cagcgcgaga cgcaagccta cccggactac gcgaacgccc acaacgcgct
     3121 cggcgtggcg ctcgtgaacc aggggaggaa cgaagaggcg ctcgccgcgt tcgtcgctgc
     3181 gacgcgcctc gatcccggcc acgcggaggc cagcaacaac gcgggcagcg tgctgcgcac
     3241 gctggaccgg cccgaggacg ccttgccctt cctgcagcgc gcggttggcg cacagccggg
     3301 gctggcggac gctcaccaga acctcggcct ggtgctgcac gcgctgcgac gctatgagga
     3361 agccgtcgcc agcttccggc aagtcatcgc tctggccccg cgcacgccct acgcgctggg
     3421 ctcgctggtg tgggccgagc tgttcgcctg cgactggcgc gagatcgggc cgcacatcgc
     3481 ggcgttgcgc gagcaggtgc gcacgggcga ggtggcggcg gccccgttca ccctgctcgc
     3541 cacctcggac tcggctgcgg agcagcgccg ctgcgcggag ctgcacgtgc gcgaaacgct
     3601 cggcggcacc cgcgaaccgc tctggcgcgg cgcgcgctac gagcatgcgc ggatccggct
     3661 ggcgtacctc tccgcggact ttcacgagca cgccaccgcc tacctcgccg cgcggctctt
     3721 cgagctgcac gaccgcagcc gcttcgagct gatcgccatc tcctacggcc cggacgaccg
     3781 cagcgccacg cgcgcgcggc tgcagcgcgc tttcgacaag ttcgtcgacg tccgagccat
     3841 gagcgacgcg caggccgcgc ggctgatcca tgagatggag gtcgacatcg cgatcgatct
     3901 caagggcctc accgccgatt cccggataac gatcctcgct caccggccgg ccccggtgca
     3961 ggcgacctat ctcggctacc ccggcaccgt gggcgcggac tacatcgact acgtcctggc
     4021 ggatcgcgtc gtcatcccgc ccagcgacga gcgcttttac actgaaaagg tcgtttgcct
     4081 gcccgattgc taccagatca acgacgcgac cgcggcgatc gccgagcgca cgccgtcgcg
     4141 cgccggggcg ggcctgcccg aacgcggctt cgtattctgc tgcttcaaca atacctacaa
     4201 gctcgggccc gggatgttcg agatctggat gcggctgctc gcccaggagc cccacagcgt
     4261 gctctggctg ctcgagcacg aagcgctcgc caggcgcaac ctccagcgaa gcgccgaagc
     4321 gcgaggcatc gacgccgcgc ggctggtgtt cgcgcccagg gttgcgcacg ccgagcacct
     4381 tgcgcggcac cggctcgcgg acctcgtcct cgacacgctg ccctacaacg cgcataccac
     4441 ggcgagcgac gcgctctggg ccggcgtgcc cgtcgtcacc tgcctgggcg cgacgttcgc
     4501 cggccgcgtc ggggcgagcc tgctgaacgc ggttggcctt ccggagctga ttacccggga
     4561 tttgtcggcg tacgaggcgc tggcactgaa gctcgcgaaa gaccgcaatt tcctgtccgc
     4621 catccgggag aagctcggcc gcaaccggct gacgcacccg ctcttcgaca gcgcgcgcac
     4681 ctgccggcac atcgaggctg cctatgagcg catgcgggat agactaggca gatgaagtat
     4741 cgcgttctcg gccgcaccgg cgtccaggtt tcccaactct gctgcggcac catggcgttc
     4801 ggcggcgacg ccgacgagaa ggaagccgcg cgcatctacc gcgcggcgcg cgaggccggg
     4861 atcaacttct tcgacaccgc cgaccagtac aacaagggac gctcggagga ggtcctcggc
     4921 gagctcattc gcggtgagcg cgacagcctg gtcctcgcga ccaagtgctt caatccgacc
     4981 ggagacgacg tcaacgcgcg cggcgcctcg cgccgccacg tgagccgcgc ggtcgaggcg
     5041 agcctgaagc gcctgggcac cgaccgggtc gagatcctgt tcctgcacca ttacgacgcg
     5101 ctcacgccgc tcgaggagca catgcgcggg ctcgaggatg tggtgcgcgc cggcaaggcg
     5161 ctctacaccg cggtaagcaa ctacgcggcg tggcagacgc aggccgcgct cggcatccag
     5221 gaacgtgccg gctgggcgcg gctgcaggcg atccagccga tgtacagcct gctgaagcgc
     5281 caggccgagg tcgagctcct gcccatggcc gcggcgaacg gcctctcggt cttcccctac
     5341 agcccggcgg ccggcggcct gctctccggc aggtacagcg agcccggcgc gcagggacgg
     5401 cacacgacca acaagatgta cgccgtgagg tacggcgagg cctgggcgca ggacgcggcc
     5461 gcgaagtttg ccgctctttg cagacaaaga aaccttcatc ccatgagcgc ggcagtggcc
     5521 tgggtcgccg cgcatcccgc ggtcaccgcg ccgatcatcg gcgcgcgcag cgccgagcag
     5581 ctgcgcgcct cgctcgactc ggtgaaagtc gacatgacgc ccgagctgcg ggcggagatc
     5641 accgcgctgt cccgcacccc gccgccggcc accgaccggc tcgaggagct caagtgatcg
     5701 gctggttcga ttgcaccgcg gtggacgagt tcgccgacgc cgtggtggcg gaccttctca
     5761 agcgcgtgcc gcccccctcg atcgaatcgg tgggaaaggt caccgccaag cgcctgaaga
     5821 gcacgaacga catgatcttc gcgcgcgccg agtcgttcgc gcgcgcgcag ccgctcaacg
     5881 tctacaagaa ggcgcatctc ggcaaccgcg tgaaatgggc gctgcgcgaa gccggctatg
     5941 ccgaggaatt cgtcgacgcg ctcacttacg agctggtcac ggtgatcacg ctcgccgcgg
     6001 gcgcacgcaa gaaaccgtga gccgcctggc gcggctcgcc ttcgacctgc gccgcgagcg
     6061 ccaggcgatc cccgatgctt tcccgaacta cgcgcgcgac ccggtggcgc tcgccacgga
     6121 ccggcgcctc aagggctggg cgcgcatcgc gggctttttc ggtgtcggcc tcgatctgct
     6181 cgccgccttg tgcggcaccg acaagtacgg cgtgcaccac tacacgccgg tgtacgaggc
     6241 gctcgcgcgc ccgctgcgcc gccagccggt gcggctcctc gagctcggcg tcggcggcta
     6301 cggccacggc ctgggcggcg aatcgctcct gatgtgggcc gcgtacttcc gccgcgggcg
     6361 catctacggg ctcgacatcg aagacaagac cgcgctgtcg cgcggccgca tcaaggtggt
     6421 ccagtgctcg cagaccgacc gcgcgcggct cgaggcgctc ggcgccgaga tcgggccctt
     6481 cgacttcgtg atcgacgacg gcagccacgt caacgcgcac cagatcgagt cgttccgcat
     6541 cctgtggccg ttcgtaaagg acggcggctg gtacgtcgtc gaggacgtgc agacatcgta
     6601 ctggccgcac tacggcggcg gcacgcccgg gtcgccgcgc cacgcttcct cctgcatcag
     6661 cttcttcaag tcgctcgccg acagcgtgaa cgagtgcgag tttctcgagc gcccggacgc
     6721 cgagatcgtg ccctcgatcg ggcatatcgc gttccaccac aacctgatcg tgctcaggaa
     6781 agacgagtcg cgccgcacct cgaacgtccc cctcggcaac gagaaagtgc gcgccgcgct
     6841 gatgaagccc cggtcgaatc cgtaaacagg accggaaaaa cagggacaga ccccattttc
     6901 ccgctgcact acctccagtg cgagaacagc gggccgtcca cgccatacac cggatccgcc
     6961 gccggcatcg gtacgcgggc aatagccgcc tggacgttct tcagcgttgt cgtggtgcag
     7021 gtgtccgggt tgctgccctc gggataggcg ccgacaacac cgagcgcgcc gcgcgcagcg
     7081 agtttcttgt ggccggtgcc ggcgggcagc acgatgacgt cgcccggctt cgccgtcacc
     7141 gtcacgccgc cttcgccgcc gaactgcacc gtgacctcgc cctcgtagat gccgagcacc
     7201 tcatggctcg tgctgtgaaa gtgatggaac gggtgcacgc cgttgcgcca cgccgctggc
     7261 cagcggttgc gcgtgaagag ctcttcaaac gcctctggtt tggcattttt cagggcttgt
     7321 ggatagatca ggagcggcca gcggggatgg ttcggaatcg aaccgtcggc cgccagcaga
     7381 tgcgtcagca ccttcatggt tgtagtctaa tcaaccgtca tgtgccgcgc gctgctttac
     7441 ctcggcgagc ccgttttgct ggataacttc ctctaccagc cggactccgc gctggtgcgg
     7501 cagtcctaca tgccgaagat gctcaacctg ctgaacctgg cagggttcgg cctgcgcgcc
     7561 tgggatccct cgagccacga ccccgaccgc gcctactcct actactcgca gagcctcccg
     7621 gttttcgacc gcaacctcaa gaacctggcc gaaaaaatcc gcaccggctg cctcctcgcc
     7681 cacgtcaggg gcgtggccta ccacacacgc gtcgagatct cgcagcagaa ctgccacccg
     7741 ttccacttca gcaacgtgcc cctggtcctg gcgcacaacg gcgacctcgc gcgcttcgcc
     7801 gagatgaagc ctttgctctc tcctttcatc aagcccgttt ttctttccca gatccacggc
     7861 accaccgaca gcgagtgggt ctacgccctc atcgtttccc agctccccga cccccaccgc
     7921 catccgagcg ccgacgagct cgccaaggcc gtcgagcgtg cactcgagat cattcgtgat
     7981 gcgcgcagga agctcggcat ctcgatctcg tcatccctca accttttcat cgccgacggc
     8041 acccagctcg ccgcggtgcg ctactgcttc gacttcgggc gctatcccac ggccgacgcc
     8101 gagcgcgtgc acgaggccaa cacgagcttc ctgtcgctct ggtacaccgc cgggcacggc
     8161 tacggcctgc acgacggcga atggaagatg acgggcggct cggacaacgc cacctccgtc
     8221 ttggtggcgt ccgagcctct tactcgagac acggcggcgt gggtggaggt gcccgagtac
     8281 tcgatgctgc acgccacgat caggaagaat cggcccgccg tcacgatccg agatctgcaa
     8341 tgatctgccc gccgtacgct ttctcgagcc gctcgctttc cttttcggcg ccccgcagcc
     8401 gcgcctggct gatgagccgc gtgagatgcc ggttggtctg gccgagcgag gcgagaaggc
     8461 tcttctgaat cacgcggtgc agcctggtcg aagtggggct gctgccgaag tagagcttct
     8521 cgaaatcctc gccgggaatc tccagcagca ccgcctgctc gcgcaccacg gcatcggagc
     8581 cgtgcacgcc cttctcgagc gcgctcatgt agccgaggag ctggccgggg ccgaggacgg
     8641 cgatgcggcg ttcgcgccgc gcgtgctgcg cgcggatttc gaccgcgccc cgcaccacga
     8701 tgaagcaggc ggtcgagggc tggccgtgat tgaagacggc gtggccgcgc gatagctcga
     8761 gcacgtgggc gatctcgagg atctcggcgg tttcgccagg gccgaagccc tcgaagaacg
     8821 gcaggtgcga aaggaaaggc ttgaggtcga aggaggcctc cttcacgcgc ttcaccttgg
     8881 ccagcggatc ggcatcgtgg ccggtccctg atttcggcgt gtctccgggg gcggcgactt
     8941 cgaggacctt ggcgttgagc gcgcgcagct tgtccgacag ggcgagggtc acggcgtgct
     9001 ggatgcgcag cgccgcgggg acgcgctggg cgacgagcga gcggaaatcg tcgcgctcga
     9061 tgaaccagcc ttcgagcttt tcgctggcgg tgacggtggc cgtgcaggtg ccgcgctcga
     9121 acagcgccat ttcgccgaac acgctgccgg ctccgagcgt cgccacggtg agcttttcgc
     9181 cgccgggcag gaggacggtg gcctcggcgg cgccttcctt caggaggtac gcgccgcgcg
     9241 agggatcgcc gtagcgcacg aggcgatccc ccttggcgtg cgtgatcgga cgggccacgg
     9301 cgagcagaag gtcgcgcgct tcgctgtcga ggcggtcaag gaaagtccgc acgagggatg
     9361 agtttacgcg ccgaggacgc ggtggacgaa ctggacgcag ctcgagcctt cgccgacggc
     9421 cgaggcaaca cgcttgacgc tgccggagcg cacgtcgcct acggcgaaca caccgggctt
     9481 gctggtttcc agaagtgagg gcgcgcgggg cagcgaccaa ccggccgccg ccaggtcctg
     9541 cgcagtgagc tcgatgccgg tgcgcacgaa gcccttgtcg tcgagcgcga ggcagccgtc
     9601 gagccaggcg gtgttgggcg aagcgccggt catgaggaag acgtgcccga tctcgcgcgt
     9661 ctcggccgcg gcacccttgc gctgccagat gacgcgctcc aggcgctcgc cgccttccat
     9721 ctcctcgatc tcggtgtgcg tgtgtagcgt gatcgccggg ttctcctcga tgcgccggat
     9781 gaggtagcgc gacatgctct ccgcgagccc cgggccgcgc accagcatgt ggacgtggcg
     9841 gcaggtattg gcgaggaata cagccgcctg tcccgcagag ttgccgccgc cgacaatcgc
     9901 gatttccttg ccgtcgcaca gcttggcctc gaggtgtgta gcggcgtagt acacgcccag
     9961 gccgacgaag cgctgcaact cggtgcactc gggcgcgcgg tacttcgcgc cggtcgcgac
    10021 cacgaccgac ttcgccagcg ccctgtgccc gtcggaaagc tggatcgcgt agggccggcg
    10081 cgagcagtcg aggcgcaagg cgtgcgacgc cacgttcacg gcggcgccga atttctgcgc
    10141 ctgcacgaag gcgcgcccgg cgagcgcgcc gccggagata ccggtcggga agccgaggta
    10201 gttctcgatc ctcgagctcg agccggcctg cccgccgtac gcgagcgcct cgatcaccat
    10261 gacgtcgagg ccctcggaag cggcatagac cgcggccgcg agcccggcgg gtcctgcgcc
    10321 caccaccaca acatcgtgca cctgggcctc atccatttcc gggttcatcc cgagaaaccc
    10381 ggcaagcgtg cggatgtccg gattgcgcag caccccgcgc ggcgcgagca ccaccggcac
    10441 ctcgtcgacg cccacgccgt ggcggcaaag cagctcttcg acgcccggat cctcgtcgac
    10501 gtccagactc atgtaggggt ggttgttgcg cgtgaggaac tcgcgcaggc gcagggtgtt
    10561 cgccgagtgg cgcgagccga ccagcgccac ctcgctctcc ccggagtcga cgagacccat
    10621 gcgccgcagg atgaaggcgc gcatgatgat ttcgctcagc tcggcgtcgg tctgcaccag
    10681 cgtgcgcagc tccgcttcgc ccaccgcgag cacctcgccc gcctccacca cgcggatgcg
    10741 cgagaaaccg gccaggccgc gcagcgtgga gagctcgccg gcgaaatccc cgggtacgag
    10801 ctgcgtgagc gactgctcgc cacgcgcgcc aggaatgaag atttcaagct tgccggacag
    10861 caccaccagc atcttgcggt ggctgtcgcc cggctcggcc agagtctcgc cggcgcggat
    10921 tctcaggcgc acgccgcgcg gctccaggcg cgcgatctgc cgcgcgtcga gccgcggaaa
    10981 catctgctcg cggcgtttct cgtagacctg gttcgccatc cgccctgctt ttcagccgcg
    11041 ctcgagggtc ttcttcgtga cggcgcagcc ctggtcgatc atggcggcga tcggcttggc
    11101 catttcgtcg ggcagcaggt cggcggtcca caccgcgcgg gtacgcgcgc ccggctccag
    11161 acggcaggcg gtcacaaagc cgggcaccag ccgcgtgtgc agcgcaccga tgtcgcgcac
    11221 cgcctcccat acgtcggtgg gtcgagcgtc gatcaggaac tcccgacgga tcgaggccat
    11281 gtcgagcgag tgtacactcg gccggttccg tgaatcagtc gatcccccag cccgccgccg
    11341 agcagatcga cccgaaccgc gtgctcgcgg tcgtcgccgc ggtggcgcgc gaagcgcggc
    11401 cgaatgtcga agttgcggtg gcgctcgaca gctcgctcga gcgcgagctc gggctcgaca
    11461 gcctcgcgcg cgtcgagctg gtcctgcgcc tggagcgcga gttcacgacc agcctgccgg
    11521 agcaggcgct cgcgtcgagc gaaacgccgc gcgacctgct gcgcttcctg ctcgcgagcc
    11581 acggccacgc gccgcaaacc gcggaccgga cagtggcgag cctctcgcag gccgagggcg
    11641 tgcgcccgcc cgaccacgcg caaacgctga ccgaggcgct cgagtactac gtcgagcgcc
    11701 agcccgaccg gctcaccgtg ttcctgtacg aggagcagaa ggaaacgcag atcacctacc
    11761 ggacgctctg ggagggatcg ctcgcttatg cggccggcct cgccgcgtcg gggctgatgc
    11821 ccgggcagac ggtggcgatc atgctgccga cctcgaagga gtacctcttc tgcttctacg
    11881 gcacgctgct cgcgggcggc atcccggtgc cgctctatcc gccggcgcgg ctcaccacca
    11941 tcgaggacca cctgacgcgc cacgtcggca tcctcaaaag ctgccgcgcg gcgctgatgg
    12001 tcacgatccc ggaggcgaag gcgatcgcgt ggctgctgcg cgcgcaagtg gaaacgctgc
    12061 gcgcggtgat ggtgccggcg gatttcccgg caagcgcaaa ggattttgtt cccgtaaaaa
    12121 caacctctgg ccagaccggc ttcctgcagt acacctcggg cagcaccggc cagccgaaag
    12181 gcgtcgtact cacccacgcc aacctgctcg ccaacgtgcg cgcgatgggc aaggcggcgc
    12241 gcgccacttc ggcggacgta ttcgtgagct ggctgccgct ctaccacgac atgggcctca
    12301 tcggcggctg cttcgcgacg atggtcctca gcttcccggt cgtgctgatg tcgccgctcg
    12361 cattcctcgc gcggcccagc agctggctgc gcgcgatcca ccgccacggc ggcacgatct
    12421 cgggcgggcc gaacttctgc tacgagcttt gcatgcgccg cattcccgag cacgagctcg
    12481 aaggggtcga cctttccacg tggcgcttcg ccttcaacgg cgccgagccg gtcagcaccg
    12541 agaccatgac cggcttcacg gagaagttct cccgctgggg attccgcaag aacgcgatga
    12601 ccccggtgta cggcctcgcc gaagcgtcgg ttggcctcgc cttcacgccg ccgggcaatc
    12661 cgtggcaggt cgatctcctc gaccgcgagc atttctccag caccggcgaa gcggtgctcg
    12721 cgcgcgagga cgatccggcg ccgctcaagg tcgtcgcgtg cggccgcccg attccggacc
    12781 atgacctgcg cgtggtcgac ggcgcggggt tcgagcttcc ggaccgctcc gaggggtcgc
    12841 tccagtttcg cggcccttcc gcgaccaccg gctactaccg caacccggaa gcgaccaagg
    12901 cgctgttcga cggcgagtgg gtgaacaccg gcgaccgcgc gtatctctcc gacggcacgc
    12961 tgtacctcac cgggcgcgag aaggacatca tcatccgcgg cgggcgcaac atcagcccgt
    13021 acgagctcga ggaggcggtc ggcgacattc cgggcgtgcg gcgcggttgc gtggcggtgt
    13081 tcggctcgct ggatccggcg agcggcaccg agcgcatcgt ggtgctggcc gagacgcgac
    13141 aaccgggcgg cgcgggcgag gcggacatca agcggcgcat caacgatctc tcgatgaacc
    13201 tgatcggcgc gccggtcgac gacatcgtgc tcgcgccgcc gcacaccgtg cccaagacct
    13261 cgagcggcaa gatccgccgc gtggcggcgc gcgactatta cgagcgcgga ccggagtcgg
    13321 tcaagccgca ggcggtgtgg ctgcagttca tccggcttgc cgccgccggc gtggcgccgc
    13381 agatccgccg cggctggcga gtggcgcgcg gcatcctgtt cgctctgcgc gcctatgcgg
    13441 ttttcttgat cacgacccca tggatattcg gagcggcggc gaccaggaac gtcggcctga
    13501 cgtggaaagt gggcggcgtc atgacgcggc tcttcctgcg cctcgccggc ctgcgggtgc
    13561 tggtgcgcgg cctggaaaac attccacccg ggcccgtggt gctgacggcg aatcacacca
    13621 gctacttcga cgcgctggtg ctggtatcga tcctcggccc gcgcaatttc tccttcatcg
    13681 cgaagcgcga gttcctcgcc aaccctctga tgcggcttct gctcaccggc ttcggctgcg
    13741 tgttcgtgga acgcttcgac gtccaggccg ccgccgggca cgccgacgag ctcgccgagg
    13801 cggcgaagcg cggccagtcg ctggcgatct ttccggaagg cacgctgctg cgccgccccg
    13861 gcctgctgcc gttccggacc ggcgcgttcc aggtcgcggc gcaggccggc atcccggtgg
    13921 tgccggtggc gatgcgcggc gtgcgctcgg tgctgcgcga cggcacctgg tacgcgcgcc
    13981 ggcacccgat ggcggtcacc ttcggcgcgc cgatcgcgcc cgagggcaac gactggaatg
    14041 cggcggtgcg gctgcgcgac ctcgtgcgcg ccgaagtgct gaagcactgc ggcgagcccg
    14101 acctggctgc tgagaatctt taggtaactg ctcttacaaa atcttcgagt gcgtcgagcg
    14161 gcgcggcatc gcggaacagc cgctcgcggc tcgactcgat gcgccggcgc agcgcctgcc
    14221 tgcgcccggc gtcggcggca agcgcggcag cgagctcgac gtaggcttcc gccgacggcg
    14281 ccaccagctc gtcgagaccc aggcggcgca gcgggccgct cgcgaggcgg ccgcgcagga
    14341 agcggccctc gcacgcgacg atcggcaggc cgcattcgag cgcatgcacg gcggtgttga
    14401 atcccgagaa gccgatcgtg tcgagataaa ggtccgcgcg ccgcagcagc gcgcggaacg
    14461 cgccggtggg ctgccagggg atgaaggtca cctggcgctc gaagtccact tccgcgcggc
    14521 ggaacgagtc gcgcaggcgc tcgcgcagct tctccgcgag cggctcggga cggctgcgga
    14581 agaacaccag gcgcgagccc ggcacgcgcc gcgcgatctc ggcgagcacc cggtcgtgct
    14641 gcggcgcgta cttgaaggcg gtaccggggc acacgagcag cagctcgtcc tttcgcagct
    14701 cgagcccgct gaacctgccg gcttcttctt tcccgggaga caacgagcaa ccgaggttcg
    14761 gcagggcaat cagtttttcg gtgtagttgg cctgcgcatc cggcggttcc agcccctccg
    14821 cggacaggta gtagtcgatc gtcggcaatc cggtggtctc gggatgtccc cagctcgccg
    14881 cctgcaccgg cgcgaggcgc atcgaggcga gcttcgcggt ggtcgcatcc atcccgatct
    14941 ccgggtaaat cacgaggtcg agcccggcgg cgtggatcaa ccccgcccat tggtcgaacg
    15001 gccgcaagcc ggcgtggaac tgcgcggcgc gcttgcgcgc gaacgccgtc tcggcgtcgc
    15061 cgccgctgcc gaggtgaaag acgtggagct cggctcgctc ggcgaggcgc tcgacccagc
    15121 cgcggaccag cgccatccag accgagtgct cgtagatgtg cgccgagacg atgccgattc
    15181 tcggcttgct cccgctcccg ggcgggggct tgctgcgggg gcgcttcgcc ggcagcgcga
    15241 ggccggcgcg cgactgccat cgccccatct gcccggcgca cagctcgccg tactcggcga
    15301 gcagctcgcg gttcggcgac tcctggtagg cgaggtagaa cggctggtgc acgccaacgg
    15361 cgaggaacgc gtcgggccgc ttgctcgcgg cgaaccactt gcgcagcgac ttgagctcgg
    15421 cggcgaatgc gcgcctgcgc tccgccggat cgacgcccgc gccgtacacc gcgggcaggt
    15481 gcgacacggc gcgcgcccag cgcgcttcgg cgtaatcggg ccgcgcctcg atcgccgcct
    15541 tgaactcggc gagcgcggcc tgccagttgc cgagccccat gtgcgcgttc cccgcctgga
    15601 ggcgggcttc ggcgagccgc gggctgagct tgatcgcgcg caacaggctg gcaagcgcct
    15661 cctcgtaggc tcgctcttcg accagcatga gcccgatgcc gtagtgcgcc tcggcgaatg
    15721 cgggctcgat gcgcgccgcc tcgcggtacg caccgagcgc ggccgggcgc tcgccgcggt
    15781 cgcgaagggc gctgccgagg ttgtggtggg ccgcggcgtt cagcggctcg agctcgaccg
    15841 cgcggcgcag caggacgatg gcctcatcga gccgcccgcg ctgggcgagg tcgctgccga
    15901 gctggaagca gtcttgcgcg ccagcgggca gcggggcctt gccgcggccg agcagccact
    15961 tcagcacggc ggggctagcg tgccgtcgac gcaggtgacg gcgtggccgc cgatttcgat
    16021 cttctcgccg attcgtaccg agacatggcc gtcgcggccg agctgcattc cttggcgcgc
    16081 gacgtaagta cttccaaatc tctcgagaag atgttgttct ttgagaaaaa cagcgacgct
    16141 aaggttgccg ctgccgcaga ccgggtcttc cggaatgctg tgcgcgggcg cgaaggagcg
    16201 gacgtggatg gcgctggcgg actcccccga agccgcaaat acggtcgcgc ccgaaacgcc
    16261 aagcgacagg ctccattccg cgagcgcggt catgtcgggc ttcaggtcgg cgagcgcgcg
    16321 cgcatcgcgc atttcgccga cgacccacac cgggccgacg tcgaccttga tcacgtcacc
    16381 gcccggtacg agcgggattg aaggaatctg cctttccatc ttctggatct tcgccggcgg
    16441 ccccttcaga aagatgcgcc cgtcgtcctc gaccgagagc tcgatcaagc cggcgccgca
    16501 ttcctgcacc agcttcttct ttttcccgac gaaaccgctt tccagtgcgg cgtgcgccga
    16561 gccgatggtc gggtgcccgg cgaacggcag ctcgtgcttc ggcgtgaaga tgcgcagccg
    16621 gtagtcggcc ttcgaggact tgagcagaaa ggtcgtttcg gaaaggttcg tccagcaggc
    16681 gatggcctgc atcgcggcgg cgtcgaggtc ttcgccaccg atcacgactg cgaccgggtt
    16741 gccgagaaag ggacgatcgg taaagacatc gacttgcttg aaacgtagct gggccatggg
    16801 aagtttttag cgccctgcgg gccgggctgt ccagctacag cgacggaaaa acgtggtctg
    16861 tccctgtttt tgtcgctact tcaggatgcg cagcaggttg tggtgcgcgt cggtgaagac
    16921 cggcaggccg ggccggtcgg cgatctcttc ggccgcctcg gcgatgcgcg gccagccgag
    16981 gagcttcggg ttgcgcgtca cgatgacctg gtcggtcgag gaataccagt actccgcgcc
    17041 gtcctccccc tcgggcacgt ccgagacgtt cagcgcctcg aggccgaact cggccgcgag
    17101 gcgcttcacc accggcagca ggtcgatgaa gcggttggtg gcctggaaca cgatcacgcc
    17161 gtccggcttg atgtgcttga ggtagatcgc catcgcctcg cgcgtcacga ggtgcatcgg
    17221 gatcgagtcg ccggagaacg cgtcgatgcc gagcacgtcg tagccgcgcg gcggctcgcg
    17281 ctcgagcgac aggcgcccgt cgccgaggat gatctccgct ttcgccgagg tgtcgtgcag
    17341 gaacgtgaat tcgcgccgcg cgatgtcgac cacgcgcggg ctgatctcgt agaacaccag
    17401 gcggtcgccg ggcttcatcc acgcggcgat caccccggcg cccaggccga tgacgccgac
    17461 ggcgagcggc ttcttcggct gcatctcgcg gagcgacgcg aacacgcgcc cgtagcccga
    17521 gcccgggctg aagtaatcgg cgggcgtgtt gcggaacgaa tcgccgagga gctgtccgcc
    17581 gtgcatgatc gcgccgtggt acatcgcgcg gtacggcacc gggttctcgt ggtcggcggt
    17641 gcgtaccacg ccgtagaaat cgcgctccat cacgcgcacg ccctcggtgt attcctgggc
    17701 gccgcgaaac acgaacagcg cgctcacgac ggtgaccgcc gcgcccaccc atcgtatttt
    17761 ctttacgaga atagtgacgg cacccagggc gaccagcgcg atgccgagct cgaaatagcc
    17821 cgccagcacg agcggcgcga cgatcgccac cagcaccgcg ccgagcgcgc cgccgagcga
    17881 gatcatcagg tagaagcgcg tcaggtgcgc cgggtcgggc ttgagccgcg cgagctcgcc
    17941 gtggcacacc atgcacgcga cgaacagccc cgcgaggtag agcggcgcgg cgagcctgag
    18001 gtcgagcgag ggaatggcgt aggccatcag cggcacgagc accaccagcg caccaacgaa
    18061 cgccggccgg atgtaccagc gcggatggtc gaacgccagg atgaagctgg cgaggtagag
    18121 cgcgagcggc agcacccaca ggaagggaac gctcgagatg ttctgggtga cgtggttggt
    18181 gaccgcgagc agcatgaccg agcccatggc agacagagca agccattgca gctggttttt
    18241 caagggaacc ggcgtcgccg ccgtccttgc ctcggtttca aagccattca cggaaagcca
    18301 ggccgtgccg gcgcagagaa tcgcgaaccc ggcgaacagg aacgaccatc cccagcccag
    18361 gctggtcagg tcgaacgccg gctcgaacag caccgggaag ccgagcagcg cgaggagcga
    18421 tgcgaggttc gaaagcgcga acaggcgata aggcaccacg gtgcgaaagc ggcgccaata
    18481 ccacgcctgg aggagcggcg tggtggtcga gagcaggaag tacggcagcc cgatcgtcgc
    18541 gccgagcagg agcaggatgc gcaggatcgg ctgctcgtcg ccctgcggct tccagccggt
    18601 cgaggcgagg atcgacaggc acagtagcga taccgcgagc aagccgacgt gcagagcagc
    18661 ctgacgacgc ggccccaagc gcgtcgtcca gtccgcataa gcgtagcccg cgagcagcac
    18721 gctctggaag aaaaccaggc acgtggtcca gaccgccgcc gagccgccga accacggcag
    18781 gatctgcttg gcgatgatcg gctgcaccag gaacagcagg aaggacgaaa ggaagatcgt
    18841 cccggcgtac gggagcgtgg ccaacaacgg gtagcggact acttgagtac tgatttcagc
    18901 gtcctgccca tctcggccgg gttgcgggtg gtcttgatcc cgcactcctc catcacggcc
    18961 agcttctcct gggcagtgcc cttgccgccg gaaatgatcg ccccggcgtg gcccatgcgc
    19021 ttgcccggcg gcgccgtcac gccggcgatg aagccgacca cgggcttctt catgttcgcc
    19081 ttcacccagc gcgcggcggc ctcttcgtcg ctgccgccga tctcgcccac catgatcacc
    19141 gcctcggtct tcgggtcgtc gttgaacatc ctgaggacgt cgatgtgctt caagccgttg
    19201 acaggatcac cgccgatgcc gacgcagctc gactggccga ggccaagctc ggtgagctgc
    19261 ccgaccgctt cataggtgag cgtgccggag cgcgacacca cgcccaccgg cccgcgcttg
    19321 tggatgtggc ccggcatgat gccgatcttg atctggtcgg gcgtgatcac gcccgggcag
    19381 ttgggaccga ccagcaatgt tctagtgccc tgcatgcggt gtttggtgcg gatcatgtcg
    19441 cgcaccggga tgccctcggt gatgcagatc acgagatcca ggtcggcgtc gatcgcctcg
    19501 tcgatcgcgg atgccgcgaa tggcggcggc acgtagatca cggagacatt ggccccggtc
    19561 tgggctttgg catccttgac cgagccaaaa atgggaatgc cttcgaagtt ct
//
LOCUS       DATBSZ010000167         4646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2213131, whole genome shotgun sequence.
ACCESSION   DATBSZ010000167 DATBSZ010000000
VERSION     DATBSZ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213131"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      <1..143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            217..2058
                     /locus_tag="VIG70_11045"
     CDS             217..2058
                     /locus_tag="VIG70_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY8251136.1"
                     /translation="MFRPIGPGALVLALVPSFVVAQEDAVVVTASRTEQRLRDAIPHT
                     TVLTQKEIRDSQAVDLPTLLRSEAGFEMAQTGGMGSVPSPLSLRGGTSARALVLIDGV
                     RIEDVGVSSTALQHIMLDQVERVEIARGNLSSLYGSNAVGGVIQIFTKRGTGAPAPYA
                     QAMAGSRNTYDVSAGYGGELGATRFNVTASRLDTKGFSAQDPALAPLSNPDPDGYRNQ
                     SISLSGSHRLNPAHELGVAFFGTAAYLDYDSSFGPPTGEHRSNQDLSMLQGWWEARFV
                     EWWKSRVTLAESKDRRTDTLNDTFANNGNTRTRQLFWDNDLRVTPAHVFSIGVEGREQ
                     VRDSGSSFSGANPQRTRDVSAGRLGYVGRLGDHTLQANYRYEDYSDFGSEGTYFLGYG
                     YDLTAAWRLTLSRGTAFRAPTFLDLDPLFLGNPDLQPERSSTNEIGLQWASERSRVRV
                     TYFDTEYEDAIVLSPAFMPQNVQTASNKGIETSYSGVLAGFDLRASLTFQDPVEQDRT
                     NLPPQQALRRAKTLAALAAHRGFGAWRLGGEWRYSGERRDQSITNSSVTVFEGAYSVL
                     DLMARYQFSKNLWFGARLENALDEEYRYVNGYNTAGRGIFFSAGWQP"
     gene            complement(2036..2899)
                     /locus_tag="VIG70_11050"
     CDS             complement(2036..2899)
                     /locus_tag="VIG70_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY8251137.1"
                     /translation="MRFIAAAFWVISFSVDAVTAIDSDGRKVSLPAPAQRIVSLAPHV
                     TEQLFAAGAGAKVVAASDYSDYPEEAKRLPRVASSGTVNLEMVLALKPDLVVAWRLEA
                     TAPALARLESLGVPVFYSEPRRLGQIPDAIEALGVLAGTGATAAPLADSLRKELARLD
                     AAYRARRPVSVFYQISERPLMTLGGGQFVSDAIALCGGRNVFADSALMAPIVNVESVL
                     AADPEAIVGGSSDWHAYWRRFPGLRAVQAGNLYVLPINEMHRHGPRAIGATRLLCEHL
                     DRARLKAASPR"
     gene            2889..4334
                     /locus_tag="VIG70_11055"
     CDS             2889..4334
                     /locus_tag="VIG70_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010114351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEY8251138.1"
                     /translation="MNRTDRFYRARRTLMIQGTCSGAGKSTLVAGLCRVLARKGTKVA
                     PFKPQNMSLNSAVAADGGEIARAQALQAQAARVESLVDMNPVLLKPSSDTHAQVIVGG
                     RPFADLPAHDYQNLKPRLLEEVLAAFHRLQEKFDAVIVEGAGSPAEVNLRANDIANMG
                     FAEAVDCPVLLVADIDRGGALAHVVGTLACLSGSERERIAGVVINRFRGDIALLEPGL
                     RWLAGETGKPVLGVLPFLNGLALDAEDALPETKPGTDHDFRVAVPVYPRISNHTDFDA
                     LRLHPRVELLWVGPDQPMPAADLIILPGSKNVRADLEFLKAQGWADAILRHVRYGGRV
                     IGICGGLQMLGAGIHDPEGVEGPAGSTEGLGLLDLETTLQREKRLRNVSGTVLGAPFT
                     GYEIHMGTSHGAAIGGIPEGAISPDGRILATYVHGIFDSPPACAAILKWAGLEAAMGV
                     DLAALREASLERLADCLEANLDTARILSSLF"
     gene            4353..>4646
                     /locus_tag="VIG70_11060"
     CDS             4353..>4646
                     /locus_tag="VIG70_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251139.1"
                     /translation="MVRDAVPLEECARALVVKLRHHGDVLLASPVISLLKARAPHLEV
                     DALVYDDTVPMLEGHPALSQLYFVGRKWRDRGALARFTLEKHLLQSLRARRYDL"
BASE COUNT          763 a         1600 c         1585 g          698 t
ORIGIN      
        1 ggcttcaggc cccggcaacc tgtgctgccc ccgcaacggt aagcgcgtca agccgcatca
       61 acacgccact gtggaagccc acgggaaggc gatgcgagcg agtagcgcaa gcccggagac
      121 cggccgacta gctggcgtcg cctcagcgag gcgaggctgt ccgtaacggc tttccggctt
      181 gcggggacgc tcgccgggga agggagagaa gttccaatgt ttcgacccat cggcccgggc
      241 gcgctggtgc tggcgctcgt tccatccttt gtcgtcgctc aggaagacgc agtcgtcgta
      301 accgcttcgc gcacagagca gcgcttgcgc gacgccatcc cgcacaccac tgtgctcacc
      361 cagaaggaga tccgcgactc gcaggcggtc gacctgccga cgctcctgcg cagcgaggcg
      421 gggttcgaaa tggcgcagac cggcggcatg ggctcggtgc cctcgccgct ctcattgcgc
      481 ggcggcacga gcgcgcgcgc cctggtcctg atcgacggcg tgcgcatcga ggacgtcggc
      541 gtcagctcga ccgcgttgca gcacatcatg ctcgatcagg tcgagcgcgt ggagatcgcg
      601 cgcggcaact tgtcgagcct gtacggctcg aacgccgtcg gcggcgtgat ccagatcttc
      661 accaagcgcg gcaccggcgc gcccgcgccc tacgcgcaag cgatggccgg gtcgcgcaat
      721 acctacgacg taagcgcagg ttacggcggc gagctcggtg cgacccgctt caacgtcacg
      781 gcgtcgcgcc tggatacgaa gggcttctcg gcgcaggatc cggcgctcgc gccgctctcc
      841 aatcccgacc cggacggtta ccgcaaccag agcatctcgc tgagcggctc gcaccggctg
      901 aaccccgcgc acgagctcgg cgtggcgttc ttcggcacgg ccgcctatct cgactacgac
      961 agctcgttcg gcccgcccac gggcgagcac cgctccaacc aggacctctc gatgctgcag
     1021 ggctggtggg aagcccgctt cgtcgagtgg tggaagtcgc gcgtcaccct cgccgagagc
     1081 aaggaccggc gcaccgacac gctgaacgac acgttcgcca acaacggcaa cacgcgcacg
     1141 cgccagctct tctgggacaa cgacctgcgc gtgacgccgg cgcacgtgtt ctcgatcgga
     1201 gtcgaagggc gcgagcaggt ccgcgacagc gggtcttcct ttagcggcgc caacccgcag
     1261 cgcacgcgcg acgtgagcgc agggcgcctc ggctacgtcg gccggctcgg cgaccacacg
     1321 ctgcaggcga actaccgcta cgaggactac tcggacttcg gctccgaagg cacgtacttc
     1381 ctcggctacg gctacgacct caccgccgcg tggcgcctga cgctgagccg cggcacggcg
     1441 ttccgcgcgc cgacgttcct cgacctggat ccgctgttcc tcggcaaccc ggacctgcag
     1501 cccgagcgct cgagcaccaa cgagatcggc ctgcagtggg cctcggagcg cagccgcgtg
     1561 cgcgtgacgt acttcgacac cgagtacgag gacgcgatcg tcctcagtcc agccttcatg
     1621 ccgcaaaacg tgcagacggc atccaacaag ggtatcgaga cgagctacag cggcgtgctc
     1681 gccggcttcg acctgcgcgc ctcgctcacc ttccaggacc cggtggagca ggaccgcacc
     1741 aacctgccgc cgcaacaggc gctgcgccgg gcgaagacgc ttgccgccct cgccgcgcac
     1801 cgcggcttcg gcgcctggcg cctgggcggc gaatggcgct acagcggcga gcggcgcgac
     1861 cagagcatca ccaactcctc ggtgaccgtg ttcgagggcg cctattccgt gctcgacctg
     1921 atggcgcgct accagttcag caagaatctc tggttcggcg cgcggctcga gaacgccctc
     1981 gacgaggaat accgctatgt gaatggctac aacaccgcgg gacgggggat cttcttcagc
     2041 gcgggctggc agccttgagc cgcgcccggt cgaggtgctc gcagagcagc cgggtggcgc
     2101 cgatcgcgcg cgggccgtgg cggtgcattt catttatcgg caagacgtaa agattcccgg
     2161 cctgcactgc tcgcagtccg ggaaagcggc gccagtacgc gtgccagtcg ctgcttccgc
     2221 cgacgatcgc ttcggggtcg gccgccagca cgctctccac gttcacgatc ggcgccatca
     2281 gcgccgagtc ggcgaagacg ttgcgcccgc cgcacagcgc gatcgcgtcg gagacgaact
     2341 gcccgccgcc gagcgtcatc agcgggcgct cggagatctg gtagaacacg ctgactggcc
     2401 gccgcgcccg gtacgcggcg tcgagccgcg cgagctcctt gcgcagcgag tcggcaaggg
     2461 gcgctgccgt ggcgccggtg ccggcgagaa cgccgagcgc ctcgatcgcg tcggggatct
     2521 gcccgaggcg cctcggctcg ctgtagaaca ccggcacgcc gagcgactcg agccgcgcca
     2581 gagccggcgc ggtcgcctcc aggcgccacg ccacgaccag gtcgggcttg agggcgagca
     2641 ccatctcgag gttcaccgtc cccgagctcg cgacgcgcgg caggcgcttg gcttcctcgg
     2701 gataatcgct gtagtcggag gcggccacga ccttggcgcc ggcgccggcg gcgaagagct
     2761 gctcggtgac gtgcggcgcc aggctcacga tgcgctgcgc gggtgccggc agcgagacct
     2821 tccggccgtc gctgtcgatg gctgtgacag cgtctactga aaaggaaatt acccagaatg
     2881 ccgcagcgat gaaccgcacc gatcgattct accgggcgcg gcgcacgctc atgatccagg
     2941 gcacctgctc cggcgccggc aagagcacgc tggtggcggg gttgtgtcgc gtgctggcgc
     3001 gcaagggaac gaaggtcgcg ccgttcaagc cgcagaacat gtcgctcaac agcgcagtcg
     3061 cagccgacgg cggcgagatc gcgcgtgcgc aggcgttgca ggcgcaggcg gcgcgagtcg
     3121 aatcgctcgt cgacatgaat ccggtgctgc tcaagccttc gagcgacacg cacgctcagg
     3181 tgatcgtcgg tggaaggccg tttgcggatt tgccggcgca cgattaccag aatctgaaac
     3241 caaggctctt agaggaggtg ctggcggcgt tccaccggct ccaggaaaag ttcgacgcgg
     3301 tgatcgtcga aggcgcgggc agcccggccg aggtgaacct gcgcgcgaac gacatcgcga
     3361 acatggggtt cgccgaggcc gtcgattgcc cggtgctcct cgtcgccgac atcgaccgcg
     3421 gcggcgcgct cgcgcatgtg gtgggcacgc tcgcctgcct gagcggctcc gagcgcgaac
     3481 gcatcgccgg cgtggtgatc aatcgcttcc gcggcgacat cgcgctgctc gagcccgggc
     3541 tgaggtggct cgcgggcgag accggcaagc cggtgctcgg cgtcctgcct ttcctgaacg
     3601 gcctcgccct cgacgccgag gacgcgctgc ccgaaacgaa accagggaca gaccacgatt
     3661 ttcgcgtcgc ggtgccggtc tatccgagga tcagcaacca caccgatttc gacgcgctca
     3721 ggctgcatcc gcgggtcgag ctcctctggg tggggccgga ccagccgatg cccgcggccg
     3781 acctgatcat cctgccgggg agcaagaacg tgcgcgcaga cctggaattc ctgaaagcac
     3841 agggttgggc cgatgcgatc ctgcgccacg ttcgctatgg cggccgcgtc atcggcatct
     3901 gcgggggcct gcagatgctc ggggctggga tccacgatcc cgaaggcgtc gaagggccgg
     3961 cgggatcgac agagggcctc ggcctgctcg acctggaaac gacgctgcag agggagaagc
     4021 gcctgcgcaa cgtcagcgga acggtcttgg gcgccccctt caccggatac gaaatccaca
     4081 tgggcacgtc gcacggcgcg gccatcggtg gcattccgga aggcgcgatt tcccccgacg
     4141 gccggattct cgcgacctac gtgcacggca ttttcgactc gccgcccgcc tgcgcggcga
     4201 ttctcaagtg ggcagggctt gaggcggcaa tgggcgtcga tctcgccgcg ctgcgcgagg
     4261 cgagcctcga gcgcctggcg gattgcctcg aggcgaatct ggacacggca cgaatccttt
     4321 cgtccctgtt ctgaccgagt aaactcggcg ccatggtgag ggatgccgtt ccgctggagg
     4381 aatgcgctcg cgcgctggtg gtcaagctgc gccaccatgg cgacgtactg ctcgcttcgc
     4441 ccgtgatttc gctcctcaag gcgcgcgcgc cgcacctgga agtcgacgcg ctggtctacg
     4501 acgacacggt gccgatgctc gaaggccatc cagcgctgtc gcagctctac ttcgtgggcc
     4561 gtaagtggcg cgatcggggc gcgctggcgc gcttcacgct ggaaaagcat ctactgcaaa
     4621 gcctgagggc gcggcgctac gacctc
//
LOCUS       DATBSZ010000168         4643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1188457, whole genome shotgun sequence.
ACCESSION   DATBSZ010000168 DATBSZ010000000
VERSION     DATBSZ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188457"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VIG70_11065"
     CDS             complement(<1..709)
                     /locus_tag="VIG70_11065"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HEY8251140.1"
                     /translation="MLYSLTVFTWIFLAALAAATATRLWLAQRQIRHVRTHRGAVPAL
                     FSESIPLAAHQKAADYTVAKARLGIVDTVIDALILLALTLGGALQWLSDLWQRAFAVD
                     AIWHGAALILTVFLVRGLLSLPLNLYRIFVIEERFGFNRMTLRLYLADLVKGLAVGAV
                     LGIPLLLAILWLMQAAGPLWWLYAWAVLVGYSLFLQMIYPAVVMPLFNKFSPLDDPVL
                     AARVEQLLSRTGFRSRGL"
     gene            708..1250
                     /gene="orn"
                     /locus_tag="VIG70_11070"
     CDS             708..1250
                     /gene="orn"
                     /locus_tag="VIG70_11070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEY8251141.1"
                     /translation="MAADPNNLVWVDMEMTGLQPDSDRVIEIAVLVTDPQLNVVATGP
                     VLVVHQPDEVLDAMDAWNKSTHAKTGLIERVRASSLTEADAERQALEFLAAHVPANAS
                     PLCGNSICQDRRFLARWMPRLEAHFHYRNLDVSTLKELVRRWKPELKGFSKEGKHEAL
                     ADILESIEELKFYRKNVMTI"
     gene            1253..2677
                     /locus_tag="VIG70_11075"
     CDS             1253..2677
                     /locus_tag="VIG70_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251142.1"
                     /translation="MRAAVIVAAAVAVLNFAGMPGQFLDGDPAAWREEARSIVLRGEL
                     SVPPEFASRFFEPGQYFVRNESNGRYYSKYGVMNALMSLPPLWAQAAAGSDISTPGTY
                     PSLLAFNLWNLALSALLAALLYRLASLYTPRVSVRVLFVIGTLYCSALWFYQRAQSSE
                     LYQVVFFAALFLCLARYLRARATGWLAGAWVFAFFLVLTRVSFGLLLPIIALLAWRQE
                     RKLTLGIVLPPLAAVALLGCISYVKFGSPLLTGYHAWRPEVTALTGSLADGLWGFLFS
                     PRFSVFLHYPLLILSLFGAAAFWRRHRPDAIAMAAVFVPFLLLTSKMPLWAGEFGYGP
                     RYLLFMVPILSLPAIPLADAIIDRIGTWRARAWAAATVACLAYSAYLQVQVNRLSFWA
                     YYDARSVLEVAYSDSAADWFRDRHVGVICADLIRYRSDLTALPWFPEFRRKVSPQIAE
                     QYVRELGRLIDRGNWYWSRPTSSS"
     gene            complement(2661..3149)
                     /locus_tag="VIG70_11080"
     CDS             complement(2661..3149)
                     /locus_tag="VIG70_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8251143.1"
                     /translation="MSAEKKLKELGIELGPVTPPVANYVNAVRAGNLLFLAGKGPAGG
                     ISGIVGKDITVEQAYGHARTVGLNLMAVMRDELGSLDRVKRIVKVLGMVNAVPGFGEQ
                     PKVINGCSDLFVEVFGERGRHARSAVGMGSLPNNIPVEIEVIVEVSAGAKKPARRVKK
                     KK"
     gene            complement(3151..3459)
                     /locus_tag="VIG70_11085"
     CDS             complement(3151..3459)
                     /locus_tag="VIG70_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251144.1"
                     /translation="MTKTSLLVAFVLLAAAGPAAPQAPAPAPSDAKASPQPPAQPAPK
                     LNLKLDNPAQYSRETPREGGGSPDVLPSLGADARTMEKAAPLSGSRTSPYPAEPSAGR
                     "
     gene            complement(3456..>4643)
                     /locus_tag="VIG70_11090"
     CDS             complement(3456..>4643)
                     /locus_tag="VIG70_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8251145.1"
                     /translation="PMIPYGVGTSLEGHILAVQGGVCIDLSQMNQVLAVHESDLDAVV
                     QAGVTRKQLNEHIKHTGLFFPVDPGADATLGGMAATRASGTNAVRYGTMRENVLSLKV
                     VLADGRIIQTSRRAKKSAAGYDLTRLFVGSEGTLGIITEVTVRLYPVQEAMSSAVCAF
                     DTVDGCTNTVIQTIQSGIPIARCDIVCEKTVDAINKYKKTRYRVAPTVFFEFHGSQAS
                     VVEQAEAVQAIAKEHGGMDFVWATKPEERSQLWQARHDAYFACLQIRPGSRAVSTDVC
                     VPISRLAECVRETMEDVRDYFAPVPLLGHIGDGNFHLMLLVDPAKPEETEIAKTFNKR
                     LIERALRMEGTCTGEHGVGMGKIGSMRLELGEDVLDLMRDLKKVFDPDNLMNPGKIVP
                     LPS"
BASE COUNT          699 a         1569 c         1569 g          806 t
ORIGIN      
        1 agaggccgcg cgagcggaac ccggtgcggc tcaggagctg ctcgacgcgc gcggcgagca
       61 ccggatcgtc gagcggtgag aacttgttga agagcggcat caccaccgcg gggtagatca
      121 tctgcaggaa cagcgagtag ccgaccagca ccgcccaggc atagagccac cacagcggcc
      181 ccgcggcctg catcagccag aggatcgcga gcagcagcgg gatgccgagc accgcgccca
      241 ccgcgaggcc cttgaccagg tcggcgaggt agaggcgcag cgtcatgcgg ttgaatccga
      301 agcgctcctc gatgacgaag atgcgataca ggttgagcgg cagggaaaga agtcctcgaa
      361 ccagaaacac cgtgaggatc agggcggctc cgtgccagat cgcgtcgacg gcgaaagcgc
      421 gctgccacag gtcggacagc cactgcagag cgccgcccag ggtgagcgcg agcaggatca
      481 gcgcgtcgat caccgtgtcg acgatgccga ggcgcgcctt cgcgacggtg tagtccgcgg
      541 ccttctgatg cgcggcgagc ggaatcgatt cgctgaacaa tgccggcacc gcgccgcgat
      601 gcgtgcgcac gtggcggatc tgccgctgcg cgagccacag ccgcgtcgcg gtcgccgccg
      661 cgagggcggc caggaagatc caagtaaaca ccgtgagaga ataaagcatg gctgcggatc
      721 caaacaacct cgtctgggtc gacatggaga tgaccgggtt gcaaccggat tccgatcggg
      781 tcatcgagat cgcggtgctg gtcaccgacc cgcagctcaa cgtggtcgcg accggcccgg
      841 tgctcgtcgt gcaccagccc gacgaggtgc tcgacgcgat ggacgcgtgg aacaagagca
      901 cgcacgccaa gaccgggctg atcgagcgcg tgcgcgcctc gagcctcacc gaggccgacg
      961 ccgagcgcca ggcgctcgag ttcctcgccg cgcacgtgcc ggcgaacgcc tcgccgctgt
     1021 gcggcaactc gatctgccag gaccgccgct tcctcgcgcg ctggatgccg cggctcgagg
     1081 cgcatttcca ctatcggaac ctggacgtct cgacgctgaa ggagctggtg cgccgctgga
     1141 agcccgagct gaaaggcttc tcgaaggagg gcaagcacga ggcgctcgcc gacatcctcg
     1201 agtcgatcga ggagctgaag ttctaccgca agaacgtgat gaccatctag gaatgcgcgc
     1261 ggccgtcatc gtcgcggcgg cggtcgccgt gttgaatttc gccgggatgc cgggccagtt
     1321 cctcgacggc gacccggcgg cgtggcgcga ggaggcgcgc agcatcgtgc tgcggggcga
     1381 gctgagcgtt ccgcctgagt tcgcctcccg cttcttcgag cccggccagt acttcgtgcg
     1441 caacgagtcg aacggccgct actactcgaa gtacggcgtg atgaacgcgc tcatgtcgct
     1501 gccgccgctg tgggcgcagg ccgccgccgg cagcgacatt tccacgcccg gcacgtaccc
     1561 gtcgctcctc gctttcaacc tctggaacct cgcgctgagc gcgctgctgg cggcgctcct
     1621 ctaccgcctg gcgagcctgt atacgccgcg cgtctcggtg cgcgtcctgt tcgtgatcgg
     1681 gacgctctac tgctccgctc tgtggttcta ccagcgggcg cagagctcgg agctctacca
     1741 ggtggtgttc ttcgccgcgt tgttcctgtg cctcgcgcgg tacctgcgcg cgcgcgccac
     1801 gggctggctc gcgggcgcct gggtttttgc tttctttctt gtcctgactc gggtctcgtt
     1861 cggcctgctc cttccgatca tcgccctcct tgcgtggcgg caagagcgca agctgaccct
     1921 ggggatcgtg ctgccgccgc tcgccgccgt cgcgctgctg gggtgcatca gctatgtgaa
     1981 gttcgggtcg ccgctgctca ccggctacca cgcgtggcgg cccgaggtga cggcgctcac
     2041 cgggagcctg gccgatggct tgtggggctt cctgttctcg ccgcgcttca gcgtgttcct
     2101 gcactacccg ctgctcattc tgtcgctctt cggcgcggcg gcgttctggc gccggcaccg
     2161 cccggatgcg atcgcgatgg cggcggtgtt cgtgccgttt ctcctgctca ccagcaagat
     2221 gccgctgtgg gcgggagagt tcggctacgg cccgcgctac ctgctcttca tggttccgat
     2281 cctttcgctt ccagccatac ctcttgccga tgcgatcatc gaccgcatcg gtacgtggcg
     2341 cgcacgggcc tgggcggccg cgacggtggc ctgcctcgcc tattccgctt acctccaggt
     2401 gcaggtgaac cggctgagct tctgggccta ctacgacgcc cgctcggtcc tcgaggtcgc
     2461 ctattcggac agcgccgccg actggttccg cgaccgccac gtcggcgtga tctgcgccga
     2521 tctcatccgc taccgcagcg atctcaccgc gctgccgtgg ttcccggaat tcaggcgcaa
     2581 ggtgtcgccg cagattgccg agcagtacgt gcgcgagctc ggccgcctga tcgaccgcgg
     2641 caactggtac tggagcaggc ctacttcttc ttcttgacgc ggcgcgccgg cttcttcgcg
     2701 ccggcgctga cttcaacgat tacctcgatc tcgaccggga tgttgttcgg gagcgagccc
     2761 atgccgaccg cggagcgcgc gtgccggccg cgctcgccga acacctcgac gaacagatcc
     2821 gagcagccgt tgatgacctt gggctgctcg ccgaagcccg ggacggcatt gaccatgccg
     2881 agcaccttga cgatgcgctt cacgcgatcg aggctgccga gctcgtccct catcaccgcc
     2941 atgaggttca ggccgacggt gcgcgcatgg ccgtaggcct gctcgaccgt gatgtccttg
     3001 cccacgatcc cggatattcc gccggcaggg cccttgcccg cgagaaacag aagattgccc
     3061 gcgcgcaccg cgttcacgta gttcgccacc ggcggcgtca cggggccgag ctcgattccc
     3121 agttccttga gcttcttttc ggcgctcacg ctagcggccc gcgctcggtt ccgcgggata
     3181 gggtgaggtc cgcgagccgg acagcggcgc ggccttctcc atggtgcgcg cgtccgcccc
     3241 gagcgagggc agcacgtcgg ggctcccgcc gccctcccgc ggcgtttccc gcgagtactg
     3301 ggcagggttg tccagcttca ggttgagctt cggggccggc tgtgccggag gctgcggcga
     3361 cgccttcgcg tcggaaggcg ccggcgcggg cgcctgcggc gcggccgggc ccgccgcggc
     3421 gaggagcacg aaagcaacga gcaatgaagt tttggtcatg acggtagtgg aacgattttc
     3481 ccggggttca tcaggttgtc gggatcgaac accttcttca ggtcgcgcat caggtcgagc
     3541 acgtcttcgc cgagctccag gcgcatcgag ccgatcttgc ccatgccgac gccgtgctcg
     3601 ccggtgcagg tgccttccat ccgcagcgcg cgctcgatga ggcgcttgtt gaaggtcttc
     3661 gcgatctcgg tctcttcggg cttggccgga tcgaccagca gcatgaggtg gaagttgccg
     3721 tccccgatgt ggccgaggag cgggacggga gcgaagtagt cccggacatc ctccatggtc
     3781 tcgcgcacgc actcggcgag gcgcgagatc ggcacgcaga cgtcggtgga caccgcgcgc
     3841 gagccggggc ggatctgcag gcaggcgaag taggcgtcgt ggcgcgcctg ccagagctgg
     3901 ctgcgttcct ccggcttggt cgcccagacg aagtccatgc cgccgtgctc cttggcgatc
     3961 gcctgcaccg cctccgcctg ctccaccacg ctcgcctgcg agccgtggaa ctcgaaaaat
     4021 accgtcggcg ccacccggta gcgggtcttc ttgtacttgt tgatcgcgtc gacggtcttc
     4081 tcgcacacga tgtcgcagcg cgcgatcggg atgccggact ggatggtctg gatcacggtg
     4141 ttggtgcagc cgtcgaccgt gtcgaacgcg cagaccgcgg acgacatcgc ttcctgcacc
     4201 gggtacaggc gcaccgtgac ctcggtgatg atgccgagcg taccctcgga gccgacgaag
     4261 agccgcgtca ggtcgtagcc cgcggcggat ttcttcgcgc ggcgcgaggt ctggatgatg
     4321 cggccgtcgg cgagcaccac cttcagcgac agcacgttct cgcgcatggt gccgtagcgg
     4381 accgcgttgg tgcccgaggc gcgcgtcgcc gccatgccgc ccagcgtcgc gtcggcgccg
     4441 ggatccaccg ggaaaaacag gccagtgtgc ttgatgtgtt cgttcagctg cttgcgcgtg
     4501 acgcccgcct gcaccacggc gtcgaggtcg gattcgtgca ccgcaagcac ctggttcatc
     4561 tgcgaaagat caatgcagac gccgccctgc accgcgagga tatgcccttc cagcgaggtg
     4621 ccgacgccgt aggggatcat cgg
//
LOCUS       DATBSZ010000169         7309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_573071, whole genome shotgun sequence.
ACCESSION   DATBSZ010000169 DATBSZ010000000
VERSION     DATBSZ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573071"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VIG70_11095"
     CDS             <1..421
                     /locus_tag="VIG70_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY8251146.1"
                     /translation="AGALPSDKLDTVVRLQDQGRVVAVVGDGVNDAAALAQSDLGIAM
                     GTGTDVAIESAAVTLVKGDLRGIAKAIRLSRATMRNVKQNLFFAFLYNALGVPVAAGV
                     LYPFFGLLLSPIFAGAAMAMSSVSVVSNALRLRRVRL"
     gene            438..644
                     /locus_tag="VIG70_11100"
     CDS             438..644
                     /locus_tag="VIG70_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEY8251147.1"
                     /translation="METVDLKVEGMDCEGCVKSVTRMLSGVPGVQTVDVSLADARARV
                     TFDPAKAGVPEMKRAVERAGYKAP"
     gene            641..1048
                     /locus_tag="VIG70_11105"
     CDS             641..1048
                     /locus_tag="VIG70_11105"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY8251148.1"
                     /translation="MKSSLQAGLKAAKAVTVDEARCIGFMGKEGMVYATPRMVSDVEY
                     TCRDFLLQHLDPGEDSVGTHVSIDHLAATPLGLGVEISATVVEVDRRKVTFEFSVRDP
                     VEEVGRGKHVRFVVETAKSRERLAAKRAKAGLS"
     gene            complement(1045..2430)
                     /locus_tag="VIG70_11110"
     CDS             complement(1045..2430)
                     /locus_tag="VIG70_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY8251149.1"
                     /translation="MRSVFLVLVLAGCTTVGPDYKRPEMPLPAEFPTQTAETGPSVAL
                     DWWRLYGDATLDELVASVHSNNADLRIAAAQVEEAEAVMRQARAALFPEITGSFTRTR
                     SRVGQLTAPPPAATAPLERNDARLVASTSFELDFWGRFGRADEAARAGLLGSQLAREV
                     VGLTLGGLTAQTYFGLRSLDAQIGVLDNSLKLRRESLDIARARAASGLAPELDVYQAQ
                     GALSDAIVQRREAARSRALLERQLAQFSGRLDLKILPGDLFVLPVPPTPPAGLPSSLL
                     ERRPDIRAAEQTMIAANAQIGVARAARFPAVSLTAFLGGQSAALEDISSSGIWGLGLG
                     IAMPLFDAGRRAAVEDQAAARYRQTLASWQRSVEGAFREVSDALVNLEQTGVSEADLL
                     ERVQAARNALELSQIRYESGYSPYLEVLDAQRTANDAEIAFVRNRQARLAFSVDLMRA
                     LGGGWNSGTEP"
     gene            complement(2427..5555)
                     /locus_tag="VIG70_11115"
     CDS             complement(2427..5555)
                     /locus_tag="VIG70_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEY8251150.1"
                     /translation="MSNFFIHRPVFAGVIAIVIVLAGLLSAKLLAVAQYPEIAPPTVL
                     ITTSYPGASAETLSRTVAAPIEEQLSGVEKLSYFSSTSSSNGNLQITVTFEPGTNVDQ
                     SVFNVNNRVQIALPRLPDEVRRNGVIVQKRSFDILLVVSLVSPGNRRDTLYLSNYASI
                     NIVDELKRLPGLADVTIFGARDYSMRVWLNPERMARLGVTTSDVAAALRAQNAQYAAG
                     RIGTEPAPPGQNIIYTVTAQGRLIEPEEFGEIVVRAQGPQGMLRLKDIARLELGALNY
                     DTSNTLDGQPTIGMATYLQPGANALEVAALVKERMKELRGGFPEGVDYQIPFDTTRFV
                     DASIREVNLTIFEAALLVLAVVFLFLQTWRATLIPMIAVPVSLIGAFAGLWMVGFSIN
                     TLTLFALVLAIGIVVDDAIVVLENVERLMRENGMPPFEAALEAMREVSGAVVAIVLVL
                     CAVFIPVAFLGGIAGQLYRQFAVTLTFAVVISGFIALTLTPALCAILMKSGHHESRIF
                     RPFNLGFAWTTQRFLGGVNLLLRRPVMSLIAFLLVLGICVFLFLRVPTSFVPSEDQGY
                     IFGNIQLPDGATLERTRKVAGELGKIAQSNPGVQNVMVINGFDLLGGGNKTNAATMFI
                     PLKHWDLRKDMPAAAVAGAIASKGSELREGMALAFNPAAIRGLGTAGGFELYLQARSD
                     PNPQRLFQVTQAFLQALRENPQLTGINSFFRPTVPQLKVEVDREKALSLGVPVQEVFE
                     ALQSTMSSLYVNDFNKFGRTYRVQMQADAPYRAQPEDLGSVYVRSAMTREMIPLKALI
                     TTSNVVGPEQLERFNGFVAARVLGSGRPGVSSGEAIRAVEQVAASALPEGYQIAWSGQ
                     AFQEKRTGRASAVAFSLAIVMVFLILAALYERWLLPFAVVLSVPFAVAGALAFVAARG
                     LENDIYFQIGLVVLIGLAAKNAILIVEFAQQGFLEGKTAAEAALNAARLRFRPIVMTS
                     LAFVLGVLPLMLSTGAGAGARRSMGTGVVGGMLAATFIATIFIPLFFVVVARRKKERR
                     PPRHSPEAAPAE"
     gene            complement(5552..6628)
                     /locus_tag="VIG70_11120"
     CDS             complement(5552..6628)
                     /locus_tag="VIG70_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8251151.1"
                     /translation="MKYFALLLCFVVAACGDSGARQEEKKAAPAAVPVTTLEMAPRTV
                     PISYEAVARTEGSREVQVRARVAGILERQLYTEGDTVKAGQPLFRIERAPFEIDLQQA
                     RAVLAQEIARRDLARGESERLKSLVDRRAISQKEADQAASSLQQTAAAVQIAQARVRQ
                     AELNLSYTDVSAPIGGITGRAMQSVGSLVSPTGESALLTTLVQGDPIWVRFSISEAEF
                     ARVRGNESIEVTLELADGKQYPHAGKLNFAGSTVDAATGTVQMRAELPNPKLDLLPGQ
                     YVRVRAIAGTRQAFVVPQTAVAQNESGRFVWIADGGKAVQRQVRTGTWLGQDWIVLDG
                     LKTGDRVIVDNLVRLRPGAPIALK"
     gene            complement(6625..7179)
                     /locus_tag="VIG70_11125"
     CDS             complement(6625..7179)
                     /locus_tag="VIG70_11125"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8251152.1"
                     /translation="MPAIHRERVLRAATSSFLARGYGSSVDDIARRAGVAKQTVYQHF
                     ANKDALFKAVAGDLAKRVLIKLEGGDTRQSLIRFGVEYRKRALGAQGIATFRTLVPEV
                     PRFRAVARDMYANSAGEMVRRLAEFLKVRLEVPDPEFSAEMLLSLLAGLDRMKRLFAV
                     PPGPETEAARAARIVDLFLKAHQR"
BASE COUNT         1176 a         2441 c         2602 g         1090 t
ORIGIN      
        1 ggcgggagca ctgccgtcgg acaagctcga caccgttgtc cgcctgcagg atcaaggccg
       61 ggtggtggcg gtggtcggtg acggcgtgaa cgacgccgcc gcactggcgc agtccgatct
      121 cggcatcgcc atgggcacgg ggaccgacgt cgccatcgaa agcgccgcgg tgacgctggt
      181 gaagggggac ctgcgcggca tcgccaaggc gatccgcctg tcgcgcgcca ccatgcgcaa
      241 cgtcaagcag aacctgttct tcgccttcct ctacaacgcg ctcggcgtgc cggtcgcggc
      301 cggcgtgctc tatccgttct tcggcctgct gctgtcgccg atcttcgccg gcgcagcgat
      361 ggcgatgagc tcggtctcgg tggtgagcaa cgcgctgcgg ctgcggcggg tgcggctatg
      421 acccttagaa tgaacgcatg gaaaccgtag acctgaaagt cgaaggcatg gattgcgagg
      481 gctgcgtgaa gagcgtgacc cgcatgctgt cgggcgtgcc cggcgtgcag acggtcgacg
      541 tatcgctcgc cgacgcgcgc gcccgggtga cgttcgatcc cgcgaaggcc ggcgtgccgg
      601 agatgaagcg ggcggtggag cgcgccggct acaaggcgcc gtgaaaagct cgttgcaagc
      661 cggcctgaag gccgccaagg ccgtcacggt ggacgaagcc cgttgcatcg gcttcatggg
      721 caaggaaggc atggtgtacg cgaccccgcg catggtgagc gacgtcgagt acacctgccg
      781 cgatttcctg ctccagcacc tcgacccggg cgaggactcg gtcggaaccc atgtctcgat
      841 cgaccatctg gcggcgacgc cattgggatt gggcgtggaa atttctgcga cggtggtcga
      901 agtcgaccgt cgcaaagtca cgttcgagtt ctcggtccgc gacccggtcg aggaagtcgg
      961 acgcggcaag cacgtgcgct tcgtcgtcga aaccgccaag agccgcgagc ggctcgcggc
     1021 gaagcgcgca aaagccggcc tgagttaggg ctccgtcccc gaattccagc ccccgcccag
     1081 cgcgcgcatc aggtccacgc tgaacgcgag gcgcgcctgg cggttgcgca cgaacgcgat
     1141 ctcggcgtcg ttggcggtgc gctgcgcgtc gagcacctcg aggtagggcg agtagccgga
     1201 ctcgtagcgg atctgcgaga gctcgagcgc gttgcgcgcc gcctgcacgc gctcgagcag
     1261 gtcggcctcg gaaacgccgg tctgctccag gttgacgagc gcgtcggaca cttcccggaa
     1321 cgcgccttcc accgatcgtt gccacgaagc gagcgtttgc cggtagcgcg ccgccgcctg
     1381 gtcctcgacg gcggcgcgcc gcccggcatc gaacagcggc atggcgatgc ccaggccgag
     1441 cccccagatg ccgctgctcg agatgtcctc cagcgcggcg ctctggccgc cgaggaaggc
     1501 ggtgagcgac accgcgggga agcgcgccgc gcgcgccacg ccgatctgcg cgttggcggc
     1561 gatcatggtt tgctcggcgg cgcggatgtc gggccggcgc tcgagcaggc tcgagggcag
     1621 cccggcgggc ggcgtcgggg gaacgggaag gacaaagaga tcccccggga ggatcttcag
     1681 gtccaaacgt ccgctgaact gcgccagctg ccgctcgaga agcgcgcggc tccgcgccgc
     1741 ttcgcggcgc tgcacgatcg cgtcggacag cgcgccttgt gcctggtaca cgtcgagctc
     1801 gggggcgagg ccggacgcgg cgcgcgcgcg ggcgatgtcg agcgactcgc gccttagctt
     1861 gagcgagttg tccagcaccc cgatctgcgc atcgagcgag cgcagcccga aatacgtctg
     1921 cgccgtcagg ccgccgagcg tcagcccgac cacttcgcgc gcgagctgcg agccgagcag
     1981 gccggcgcgc gccgcctcgt cggcgcgccc gaagcgtccc cagaaatcga gctcgaacga
     2041 ggtcgaggcg accaggcgcg cgtcgttgcg ctcgagcggc gcggtggcgg ccggcggcgg
     2101 cgcggtgagc tggccgacgc gcgagcgcgt gcgcgtgaag ctgccggtga tctcggggaa
     2161 gagcgccgcg cgcgcctggc gcatcacggc ttcggcttcc tccacctgcg ccgccgcgat
     2221 cctcaggtcg gcgttgttgc tgtgcaccga ggcgaccagc tcgtccagcg tcgcgtcgcc
     2281 gtagagcctc caccaatcga gcgcgaccga aggacccgtc tccgcggtct gggtcggaaa
     2341 ctcagccggc aacggcatct cgggccgctt gtagtccgga ccgaccgtgg tgcagccggc
     2401 aagaaccagg acgaggaaaa cgcttctcat tcggcgggcg cggcttcggg cgagtgacgc
     2461 ggcgggcggc gctccttttt ccgccgcgcc acgacgacga agaagagcgg aatgaagatc
     2521 gtggcgatga aggtggcggc gagcatgccc ccgaccacgc cggtgcccat cgagcgccgg
     2581 gcgccggcgc cggcgccggt cgacagcatc agcggcagca cgccgagcac gaaggcgagc
     2641 gaggtcatga cgatcggccg gaaccgcagc cgcgcggcgt tgagcgcggc ctcggccgcg
     2701 gtcttgccct cgaggaagcc ctgctgtgcg aattccacga tcaggatggc gttcttcgcc
     2761 gccaggccga tcagcaccac cagcccgatc tggaagtaga tgtcgttctc cagcccgcgc
     2821 gcggcgacga acgcgagcgc gccggcgacc gcgaacggca ccgacagcac caccgcgaag
     2881 ggcaggagcc agcgctcgta gagcgccgcg aggatcagga acaccatcac gatcgcgagc
     2941 gagaacgcca ccgccgaggc gcggccggtg cgcttttcct ggaacgcctg ccccgaccac
     3001 gcgatctggt agccctcggg cagggccgag gccgccacct gctccaccgc gcggatcgct
     3061 tcgcccgagg acacgcccgg gcggccgctg ccgagcacgc gcgccgcgac gaagccgttg
     3121 aagcgctcga gctgctcggg cccgactacg ttcgaggtgg taatgagtgc tttcagcggg
     3181 atcatctcgc gcgtcatcgc cgagcgcacg tacacgctgc cgaggtcctc cggctgcgcc
     3241 cggtacggcg cgtcggcctg catctgtacg cggtaggtgc ggccgaactt gttgaagtcg
     3301 ttcacgtaga gcgacgacat ggtgctctgc agcgcctcga acacctcctg caccgggacg
     3361 cccagcgaga gcgctttctc gcggtcgact tccaccttga gctgcggcac ggtcggccgg
     3421 aagaacgagt tgatgccggt gagctgcggg ttctcgcgca gcgcctgcag gaaggcctgc
     3481 gtgacctgga acaggcgctg cgggttcggg tcgctgcgcg cctggagata gagctcgaag
     3541 ccgcccgcgg tgccgaggcc gcggatcgcc gccgggttga aggcgagcgc catgccttca
     3601 cgcagttcag atcctttcga ggcaatagct ccggctaccg ccgcggcggg catgtccttg
     3661 cgcaagtccc agtgcttgag cgggatgaac atggtcgcgg cgttggtctt gttgccgccc
     3721 ccgagcaggt cgaagccgtt gatcaccatc acgttctgca cgccggggtt cgactgcgcg
     3781 atcttgccca gctcgccggc caccttgcgc gtgcgctcga gcgtcgcgcc gtcggggagc
     3841 tggatgttgc cgaagatgta gccctggtct tcggagggaa cgaaactggt tggcacgcgg
     3901 agaaagagga acacgcagat tccgagcacc agaaggaatg cgatcagcga catcacagga
     3961 cggcgcaaca acagattcac gcccccgagg aagcgctgcg tggtccaggc gaagccgagg
     4021 ttgaacggcc ggaaaatgcg cgactcgtgg tgcccgctct tcatcaggat cgcgcacagc
     4081 gccggggtga gcgtgagcgc gatgaacccc gagatcacca ccgcgaaggt gagggtgacc
     4141 gcgaactgcc ggtagagctg gccggcgatg ccgccgagga aagcgaccgg gatgaagacg
     4201 gcgcacagca cgagcacgat cgccaccacc gcgcccgaga cctcgcgcat cgcctccagc
     4261 gcggcctcga acggcggcat gccgttctcg cgcatcaggc gctcgacgtt ctcgagcacc
     4321 acgatggcgt cgtcgaccac gatgccgatg gcgagcacca gcgcgaacag cgtgagagtg
     4381 ttgatggaga agccgaccat ccacaggccg gcgaaggcgc cgatcagcga caccggaacc
     4441 gcgatcatcg gaatgagcgt ggcgcgccag gtctgcagga acaggaacac caccgccagc
     4501 accagcagcg cggcctcgaa gatcgtcagg ttcacttccc tgatcgacgc gtcgacgaag
     4561 cgcgtggtgt cgaaggggat ctggtagtcc acgccctccg ggaagccgcc acgcagttct
     4621 ttcattcttt cctttacgag agcagcaact tccagcgcgt tggcgcccgg ctgcaggtag
     4681 gtcgccatgc cgatggtcgg ctggccgtcg agcgtattgg aggtgtcgta gttcagcgcg
     4741 ccgagctcca gccgcgcgat gtccttcaac cgcagcatcc cctgcggtcc ctgcgcccgc
     4801 accacgatct cgccgaactc ctcgggctcg atcagccgcc cctgcgcggt caccgtgtag
     4861 atgatgttct gtccgggcgg cgcgggctcg gtgccgatgc ggcccgcggc gtactgcgcg
     4921 ttctgcgcgc gcagcgcggc cgcgacgtcg gaggtcgtca ccccgaggcg cgccatcctc
     4981 tcggggttga gccacacgcg catcgagtag tcgcgcgcgc cgaagatggt gacgtcggcg
     5041 aggccgggca ggcgcttcag ctcgtcgacg atgttgatcg aggcgtagtt ggagaggtac
     5101 agcgtgtcgc gccggttgcc gggcgagacc agcgacacca ccagcaggat gtcgaacgag
     5161 cgcttctgca cgatgacgcc gttgcgccgc acctcgtcgg gcagcctcgg cagcgcgatc
     5221 tgcacgcggt tgttcacgtt gaacaccgac tggtcgacgt tggtccccgg ctcgaaggtg
     5281 acggtgatct gcaggttgcc gttcgaggag gaggtcgagc tgaaatacga gagcttctcc
     5341 acgcccgaga gctgctcctc gatcggggcg gcgacggtgc gcgacagcgt ctcggcgctc
     5401 gcgccgggat aggaggtggt gataagcacc gtcggcggcg cgatctcggg gtactgcgcg
     5461 accgcgagca gcttcgccga cagcaagccg gcgagcacga tcacgatcgc gatcacgccg
     5521 gcgaacaccg gccgatgaat gaagaaattg ctcatttcag ggcgatggga gcgcccgggc
     5581 gcaggcgaac caggttatcc acgatcaccc ggtcgccggt cttgaggccg tcgagcacga
     5641 tccagtcctg tccaagccag gttccggtgc gcacctggcg ctgcaccgcc ttgccgccgt
     5701 cggcgatcca gacgaagcgc ccgctctcgt tctgcgcgac cgcggtctgc ggcacgacga
     5761 aggcctgccg cgtgccggcg atcgcgcgca cgcgcacgta ctgcccgggg agcaggtcga
     5821 gcttcgggtt gggcagctcg gcgcgcatct gcaccgtgcc ggtggcggca tcgaccgtgg
     5881 agccggcgaa gttgagcttg ccggcgtgcg ggtactgctt gccgtcggcg agctcgagcg
     5941 tgacctcgat gctctcgttg ccgcgcacgc gcgcgaactc ggcttccgag atcgagaagc
     6001 gcacccagat cgggtcgccc tgcacgagcg tggtgagcag cgcggactcg ccggtgggcg
     6061 acaccaggct gccgaccgac tgcatcgcgc gcccggtgat gccgccgatc ggcgcgctca
     6121 cgtcggtgta ggacaggttg agctcggcct ggcgcacgcg cgcctgcgcg atctgcaccg
     6181 ccgccgcggt ctgctgcagc gaggaggcgg cctggtcggc ctccttctgg ctgatggcgc
     6241 ggcggtcgac caggcttttc agccgctcgg attcgccgcg cgcgaggtcg cggcgcgcga
     6301 tctcctgcgc gagcaccgcg cgcgcctgct gcaggtcgat ctcgaacggc gcgcgctcga
     6361 tgcggaacag cggctgcccg gccttgaccg tgtcgccctc ggtgtacagc tggcgctcga
     6421 ggatcccggc cacgcgcgcg cgcacctgca cctcgcgcga gccctcggtg cgcgccaccg
     6481 cctcgtaact gatcggcacg gtgcgcggcg ccatctcgag cgtggtgacc ggcaccgcgg
     6541 ccggcgccgc tttcttttcc tcttgcctgg ccccgctgtc gccgcaggcg gcgaccacga
     6601 agcagagaag gagcgcaaag tacttcatcg ttggtgagcc ttcaggaaga gatcgacgat
     6661 gcgagcggcg cgcgcggctt cggtttcggg gccgggcggc accgcgaaca ggcgcttcat
     6721 gcgatccagg ccggcgagca gggagagcag catctcggcc gagaactccg ggtcgggaac
     6781 ctcgagcctg accttcagga actcggcgag ccgccgcacc atctcgcccg ccgagttggc
     6841 gtacatgtcg cgcgccacgg cgcgaaagcg cggcacctcg ggcaccaggg tgcggaaggt
     6901 ggcgatgccc tgcgcgccga gcgcgcgctt gcggtattcg acgccgaagc ggatgagact
     6961 ctggcgcgtg tcgccgcctt cgagcttgat cagcacgcgc ttggcgagat cgccggccac
     7021 cgccttgaac agcgcgtcct tgttggcgaa gtgctggtag acggtctgct tggcgacgcc
     7081 ggcgcggcgc gcgatgtcgt cgacgctcga gccgtagccg cgcgcgagga acgaggaggt
     7141 ggcggcgcgc agcacgcgct ctcggtggat ggcaggcatg ggggggagtc ttcgaactag
     7201 actgatcagt ctagcagagg tgtgccgcaa aggaaaaagg ggtcaggacc ctttttgacg
     7261 aaaagggtcc tgaccccttt tttcgctacc atcggttcga tggacgggc
//
LOCUS       DATBSZ010000170         2777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1530186, whole genome shotgun sequence.
ACCESSION   DATBSZ010000170 DATBSZ010000000
VERSION     DATBSZ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530186"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VIG70_11130"
     CDS             <1..288
                     /locus_tag="VIG70_11130"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY8251153.1"
                     /translation="SSLARLPLDIRGTVFQRRVWDELRRIPRGETRTYQEIARAIGAP
                     AAVRAVGSACGANPVALVVPCHRAVRTDGGMGGYAWGIARKKKLLALEKKS"
     gene            289..1137
                     /locus_tag="VIG70_11135"
     CDS             289..1137
                     /locus_tag="VIG70_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8251154.1"
                     /translation="MRRADTARLVLLAAIWGASFLFVRIAAPVLGPVLTAELRTLIAG
                     IALAVYFAFAGFDPEWRRWGRHYVVVGILSSAVPFLLWAYAALSLTAGLLSVLNATAP
                     MWGALCSALLLRERLNARRILGLVIGMAGVALVTRPGTGSELYYPAIVAALVAAFCYG
                     LVAAYIKRWARGAPSRGMAVGSQLAAGILLIPFIALWPPAAVPTPGVTASILALGLVC
                     GAIAYLLYFRLIADIGATGALTVTYLIPVFGVLWGALFLGETVSALMLVGAALVILGT
                     VFILRT"
     gene            1225..>2777
                     /locus_tag="VIG70_11140"
     CDS             1225..>2777
                     /locus_tag="VIG70_11140"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEY8251155.1"
                     /translation="MNTYPHLFQPLDLGIATLPNRIMMGSMHTGLESRRDGMERLAAF
                     YAERARGGAALIVTGGFSPNEAGNLSAHRAEFSTAEDSARHKPIPRAVHDAGGRIVLQ
                     LLHSGRYGFHERIVAPSALKSPINPHTPREMTPREIEQTIDDFARAAALAREAGYDGV
                     EVMGSEGYLITQFLAQRTNRRGDEWGGPLENRVRFGAEIVRRTRAEAGRDFIIVYRIS
                     ALELVEGGLEADEIVQVGKAVEAAGATILNSGIGWHEARIPTIAQAVPRGAFAWATRG
                     LKQAVRIPVVASNRINAPEVAEAIIARGDADMVSLARAMLADPEFARKAAAGDRAGIN
                     ICIACNQACLDHYFIGQPASCVVNPRAGRETKLVFLKTKRKKTIAVVGGGPAGLSCAA
                     HAAERGHDVTLYEKAAELGGQFNLAKVIPGKQEFGESIAYFSERLKRAGARVVLGKAP
                     SEAELQCFDDVVNATGVDPRTPPIPGIDSPNVVSYVDVLRDAVKPGRNVAIIGMGGIG
                     FDVALYLLER"
BASE COUNT          415 a          951 c          941 g          470 t
ORIGIN      
        1 tcgagcctag ccaggctgcc gctcgatatc cgcggcaccg tgttccagcg gcgcgtctgg
       61 gacgagctgc gccgcatccc gcgcggcgaa actcgcacct accaggaaat cgcccgcgcc
      121 atcggcgcgc ccgccgcggt acgcgcggtc ggcagcgcgt gcggcgcgaa cccggtggcg
      181 ctggtcgtgc cgtgccaccg cgcggtgcgc accgacggcg ggatgggcgg ctacgcctgg
      241 ggcattgctc gcaagaagaa gttgcttgcc ctggagaaga agtcctagtt gaggcgcgcg
      301 gacaccgcgc gcctggtact gctcgccgcg atctggggcg cttcgtttct tttcgtgcgg
      361 atcgccgctc cggtcctcgg cccggtgctc accgccgagc tgcgcacgct gatcgccggc
      421 attgcgctgg cggtgtattt cgcctttgcc ggtttcgatc ccgagtggcg ccgctgggga
      481 cgccactacg tggtcgtcgg gattctcagc tcggccgtgc cgttcctgct ctgggcctat
      541 gccgctctca gcctgacggc gggcctgttg tcggtgctga acgcgaccgc gccgatgtgg
      601 ggtgcgctgt gctcggcact gcttctgcgc gagcggctga acgcccggag aatcctgggg
      661 ctggtcatcg gcatggcggg cgtcgctctg gtcacccgtc ccggaaccgg ttcggagctc
      721 tactaccccg caatagtcgc ggcgctcgtc gccgccttct gctacgggct ggttgccgcc
      781 tatatcaagc gctgggcaag gggcgcgcct tcgcgcggca tggccgtagg ctcgcaactt
      841 gccgccggca tcctcctcat tcccttcatc gcgctctggc cgcccgcggc cgtgccgacg
      901 ccgggcgtga cggcgagcat cctcgccctc ggcctcgttt gcggcgccat tgcctacctc
      961 ctctacttcc gcctgatcgc ggatatcggc gcgaccgggg cgctcaccgt cacctacctc
     1021 atcccggtgt tcggcgtgct ctggggtgcg ctgttcctcg gcgaaactgt atcggcgctg
     1081 atgctcgtgg gcgcggcgct tgtgatcctc ggtacggtgt tcatcctgcg cacctagtca
     1141 gaacaaaatc agggacagac cacgttttcc gcgaaaatgc ggatattggc cacaagggaa
     1201 aacgtggtct gtccctgatt gacgatgaac acctatccgc acctgttcca gccgctggac
     1261 ctcggcatcg cgacgctgcc gaaccgcatc atgatgggct cgatgcatac ggggctcgag
     1321 tcgcggcgcg acggcatgga gcgcctggcc gcgttctatg ccgagcgcgc tcgaggcggt
     1381 gccgcgctca tcgtgaccgg cggtttttcg ccgaacgagg cgggcaacct gagcgcgcat
     1441 cgggccgagt tttcgacggc cgaagactcg gcaaggcaca agccgattcc gcgcgcggtg
     1501 cacgacgccg gcgggcgcat cgtgctgcag ctcctgcatt ccgggcgcta cggctttcac
     1561 gagcgcatcg tcgcgccctc ggcgctgaag tcgccgatca atccgcacac accgcgggaa
     1621 atgacgccgc gggagatcga gcagacgatc gacgatttcg cgcgcgcagc cgcccttgcc
     1681 cgcgaggcgg gctacgacgg cgtcgaggta atgggctccg agggctacct catcacccag
     1741 ttcctcgccc agcgcacgaa ccgtcgcggc gacgagtggg gcgggccgct cgagaaccgg
     1801 gtgcgcttcg gcgccgagat cgtgcggcgg acgcgcgccg aggcgggccg ggacttcatc
     1861 atcgtctacc ggatttcggc gctcgagctc gtggaagggg gtctcgaggc ggatgagatt
     1921 gtccaggtcg gaaaagccgt cgaagccgcg ggcgcgacga tcctgaacag cggcatcggc
     1981 tggcacgagg cgcgcatccc caccatcgcg caggcggtgc cgcgcggcgc cttcgcgtgg
     2041 gcaacgcggg gcctcaagca agcggtgcgc atccctgtgg ttgcgtcgaa ccgcatcaac
     2101 gcgcccgagg tggcggaggc gatcatcgcc cgcggcgacg ccgacatggt gtcgctcgcg
     2161 cgcgcaatgc tcgccgaccc cgagttcgcc agaaaagccg ctgccggcga ccgcgcgggc
     2221 atcaacatct gcatcgcctg caaccaggcc tgcctcgacc actacttcat cggtcagccg
     2281 gcgagctgcg tggtcaatcc tcgcgcgggc cgcgaaacga aattggtctt cttgaaaacg
     2341 aaaagaaaaa agaccatcgc agtcgtcggc ggcggcccgg ccggcctgtc gtgcgccgcc
     2401 cacgccgccg agcgcggcca tgacgtcacc ctctatgaga aggcggcgga gctcggcggc
     2461 cagttcaacc tcgccaaggt gatccccggc aagcaggagt tcggcgaatc gatcgcctac
     2521 ttttcggagc ggctgaagcg cgcgggcgcg agggttgttc tgggaaaggc accctcggag
     2581 gccgagctgc agtgcttcga cgacgtggtc aacgccaccg gcgtcgatcc gcgcacgccg
     2641 ccgatccccg gcatcgatag cccgaacgtg gtgagttatg tcgacgtgct gcgcgacgca
     2701 gtgaagcccg gccgcaatgt cgcgatcatc ggcatgggcg gcatcggctt cgacgtcgcg
     2761 ctctacctcc tcgaacg
//
LOCUS       DATBSZ010000171         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_95831, whole genome shotgun sequence.
ACCESSION   DATBSZ010000171 DATBSZ010000000
VERSION     DATBSZ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95831"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /gene="rsmA"
                     /locus_tag="VIG70_11145"
     CDS             <1..578
                     /gene="rsmA"
                     /locus_tag="VIG70_11145"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY8251156.1"
                     /translation="KLEAIELDRDLASGLAAKFPPFRLTVHCADALEFDFARFPSGMR
                     LVGNLPYNVSTPLLFHLARYAPQVRDMHFMLQLEVVQRMVAAPSTPAYGRLSVSLQAR
                     FQMKKLFTVSRGAFTPPPKVESAVVRMEPLAWQLPIDEDLLRRAFSARRKTLRNALPE
                     IDFAAAGIDPKLRPENLSPEDYSRLSMNSIL"
     gene            complement(545..931)
                     /gene="gloA"
                     /locus_tag="VIG70_11150"
     CDS             complement(545..931)
                     /gene="gloA"
                     /locus_tag="VIG70_11150"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEY8251157.1"
                     /translation="MRMLHTMLRVGDMQRSVDFYTKTLGMKLLRTTDRPEQKYSLAFV
                     GYDDEERAAVLELTYNYGVGRYELGTAFGHIALGVPDVKAACERVRKSGGKVTREPGP
                     VKGGTSVIAFVEDPDGYKIEFIERRE"
     gene            complement(928..1590)
                     /locus_tag="VIG70_11155"
     CDS             complement(928..1590)
                     /locus_tag="VIG70_11155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY8251158.1"
                     /translation="MDRRAAKSANLIRLGGRSIEYRFVRRRRRTLGITIDADGLKVSA
                     PLRAPWRDIEGFVREKERWILAKLDEWAVAPRPAVLRGVRGERLPLFGKMVELERDDS
                     LQNLLKWLKAAALEALRPRVAHYAALLGRPAPRLRLSNARTQWGVCTEDGTIRLSWRL
                     VHIEPALADYVVAHEIAHLAELNHSPRFYRLLESLYPDWPAARERLELAGAALPILKE
                     PK"
     gene            complement(1557..2222)
                     /locus_tag="VIG70_11160"
     CDS             complement(1557..2222)
                     /locus_tag="VIG70_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8251159.1"
                     /translation="MIVTPPYAVLALLTFPLPRMARYRLISGWSRLVIFLSKVILRID
                     WQVEGRENLPARPSVILSKHQSAWETMAFQLIFPPQVLVLKRELLWIPFFGWGLALMS
                     PIAIDRSRGLAALRAMARRGRERLEQGFWVVVFPEGTRVAPGAARDYHPGGAWLACAS
                     GAQVVPVAHNAGLLWPRNAFLKRSGTVTVRIGPPIDAANRDAKTVNELARTWIEEQQK
                     ALT"
     gene            complement(2272..2439)
                     /locus_tag="VIG70_11165"
     CDS             complement(2272..2439)
                     /locus_tag="VIG70_11165"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY8251160.1"
                     /translation="MVGDAQRDLEAAAAAGAKPVLVLTGKGIETRNEGKLPPGTRVFA
                     DLAAFAAQMAP"
BASE COUNT          372 a          854 c          837 g          387 t
ORIGIN      
        1 ccaagctcga ggcgatcgag ctcgatcgcg acctcgcctc tggcctggcg gcgaagttcc
       61 cgcccttcag gctcacggtg cactgcgccg acgcgctcga gttcgacttc gcgcgcttcc
      121 cgtccgggat gcggctcgtc ggcaacctgc cgtacaacgt ctcgacgccg ctgctgttcc
      181 acttggcgcg ctacgccccg caggtgcgcg acatgcattt catgctgcag ctcgaggtgg
      241 tgcagcgcat ggtggcggcg ccttcaacgc cggcctacgg gcgcctgtcc gtttccctgc
      301 aggcccggtt ccagatgaag aagctcttca ccgtgtcgcg cggcgcgttc acgccgccgc
      361 cgaaagtgga atccgcggtg gtcaggatgg agccgctcgc gtggcagctc ccgatcgacg
      421 aggacctgct gcgccgggcg ttctccgcgc ggcgcaagac cctgcgcaac gcgctgcccg
      481 agatcgattt cgccgccgcg ggcatcgatc cgaagctgcg gcccgagaat ctctcgccgg
      541 aagactactc gcgtctctcg atgaactcga tcttgtagcc gtccgggtcc tcgacgaacg
      601 cgatcacgct cgtgccgcct ttcacggggc cgggctcgcg cgtgaccttt ccgccgctct
      661 tgcgcacgcg ctcgcaggcg gccttcacgt ccggaacgcc gagcgcaata tgcccgaagg
      721 cggtgccgag ctcgtagcgc ccgacgccgt agttgtaggt gagctcgagc accgctgcgc
      781 gctcctcgtc gtcgtagccg acgaaggcga gcgaatattt ctgctcggga cggtcggtgg
      841 tgcgcaagag cttcatgccg agggtcttgg tgtagaagtc gaccgagcgc tgcatgtcgc
      901 cgacgcgaag catggtatgg agcattctca tttcggttcc ttcaggatgg gcaaggcggc
      961 gccggcaagc tcgaggcgct cgcgcgcggc cggccagtcc ggatagagcg attcaagcag
     1021 gcggtagaag cgcggcgagt ggttgagctc ggccaggtgg gcgatctcgt gcgccaccac
     1081 gtagtcggcg agcgcgggct cgatgtggac caggcgccac gaaagacgaa tcgtcccgtc
     1141 ttcggtgcac actccccact gcgtgcgggc gttcgagagc cgcaggcgcg gcgccggtcg
     1201 tccgagcagc gcggcgtagt gcgcgacgcg cggacgcagc gcctcaagcg cagcggcttt
     1261 cagccacttc agaagattct gcaagctgtc gtcccgctcg agctcgacca ttttgccgaa
     1321 gagcggcagc cgctcgcctc tcacgccgcg cagtaccgcg ggccgcggcg ccaccgccca
     1381 ttcgtccagc ttggcgagga tccagcgctc cttctcgcgc acgaatccct cgatgtcgcg
     1441 ccacggcgcg cgcagcggcg ccgagacttt caggccgtcg gcgtcgatcg tgatgccgag
     1501 cgtacgccgg cgccggcgca cgaagcgata ttcgatgctg cgcccgccga gccggatcag
     1561 gttagcgctt tttgctgctc ttcgatccat gtcctggcca gctcgttcac ggtcttggcg
     1621 tcgcggttcg cggcgtcgat cggcggcccg atgcgcacgg tgaccgtgcc ggaccgcttg
     1681 aggaacgcgt tgcgtggcca gagcaggccc gcgttgtgcg ctaccggcac cacctgcgcg
     1741 ccgctcgcgc aggcgagcca cgcgccgccg gggtggtagt cgcgcgccgc gccgggggcg
     1801 acgcgcgtcc cctcgggaaa caccaccacc cagaagccct gctccaggcg ctcgcggccg
     1861 cggcgcgcca tcgcgcgcag cgcggcgagg ccgcgcgaac ggtcgatcgc gatcgggctc
     1921 atcagcgcga gtccccagcc gaagaacggg atccacagga gctcgcgctt cagcaccagg
     1981 acctggggcg gaaagatgag ctggaacgcc atcgtttccc acgccgactg gtgcttcgac
     2041 agtattacgg aggggcgcgc gggcaggttc tcgcgcccct cgacctgcca gtcgatccgc
     2101 aggatgacct tggacagaaa aatcaccagg cgcgaccagc ccgaaatcag ccggtagcgc
     2161 gccatgcgcg gcaggggaaa cgtgagcagc gcgagcaccg cgtagggcgg cgtgacgatc
     2221 atgagaccga gcgcgaacag cgccgagcgg agtatcgtca cggcgcccgg gtcacggcgc
     2281 catttgcgcg gcgaacgccg cgaggtcggc gaagacgcga gtcccgggcg gcagcttgcc
     2341 ttcgttgcgg gtctcgatcc ctttgcccgt gagcaccagg accggcttcg cgcccgccgc
     2401 ggcggcggct tcgagatcgc gctgcgcgtc gcccaccatg cacacctcgg
//
LOCUS       DATBSZ010000172        18718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_163506, whole genome shotgun sequence.
ACCESSION   DATBSZ010000172 DATBSZ010000000
VERSION     DATBSZ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18718
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163506"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..986)
                     /locus_tag="VIG70_11170"
     CDS             complement(15..986)
                     /locus_tag="VIG70_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8251161.1"
                     /translation="MTSNLGSRKTFLGVALAVAASTAFAWPTKPITFVVPYPPGGGTD
                     VIARIVQEPLSKQLGQPIVIENRGGAGGSLGTGVAAKANPDGHTLLFTLSSHSINPAI
                     YKSLPFDTERDFRPVSTICSLPQLFAVHPGTPYQTFKELVAYTKGHPGKLDFASVGVG
                     SPSHMAGELLELKLGIHMVHIPYRGGGPAVAATMAGDVPLLIVSIPAAMGQVRAGRLR
                     PLAVSTAKRTPILPDVPTIAEATGLKDFEVDSWYAVFAPAKTPDEVVARMNKEIATVV
                     ARPDVRAKLLEQGADGMSSTPERLGQMVKREIAEWRAVVKHANIEAE"
     gene            1032..1430
                     /locus_tag="VIG70_11175"
     CDS             1032..1430
                     /locus_tag="VIG70_11175"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEY8251162.1"
                     /translation="MESIQRGVVGSAQLVVGEEHTAPRVGSGKVHVLATPVMINLIEA
                     AALAAIEHLLPPGHQSLGTRLDVRHIAATPVGMKIFANATVESVSGRTVTFRVEARDE
                     KELIGDGTHERVVVNVEKFDARVQRKLARG"
     gene            complement(1416..2030)
                     /locus_tag="VIG70_11180"
     CDS             complement(1416..2030)
                     /locus_tag="VIG70_11180"
                     /inference="COORDINATES: protein motif:HMM:NF044451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8251163.1"
                     /translation="MEVNDARLLQGSETQKTLRREARKIFAKKGYSGANTNELVQRAR
                     VTKGALYHHFANKRELYHSVVEDMERELVERLEDAATAAAGGRRDPWERLRAMCRAYL
                     DACRDPALARMLVLEAPVVLGWKTWCDLEQKYEVAAFARCLEEANRDAGALPEPAETL
                     AQVILGALTTGARVIATSADPSAARADIQKTIDRLIAGLRSTAR"
     gene            2087..2362
                     /locus_tag="VIG70_11185"
     CDS             2087..2362
                     /locus_tag="VIG70_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEY8251164.1"
                     /translation="MPKYVIERELPGAGKLSRDELRQVAQKSNGVIETLGPKIQWVES
                     FVVDDKLYCVYNAASPKIIEEHAKCGGFPANRISQVRAMMDPTTAEA"
     gene            complement(2365..2997)
                     /locus_tag="VIG70_11190"
     CDS             complement(2365..2997)
                     /locus_tag="VIG70_11190"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEY8251165.1"
                     /translation="MTMLVIATVAFIATHFVTSTPLRPALVNAMGEWPYRGVYSLVAF
                     VTLGWMVWAYASAPRELLWSGFPNTPYWAMPVAFVLIACGYWRNPTMVGADKLLKSED
                     PARGMIRITRHPIMWGIMLWAGAHIVARGDLKSLVFFGGLLVVALVGTLSMDSRKKAN
                     PDWPRFAAVTSHVPFVAIAQGRNRIVWREIGWLRPAIGLAAFFAVLLFHP"
     gene            complement(3019..5469)
                     /gene="pepN"
                     /locus_tag="VIG70_11195"
     CDS             complement(3019..5469)
                     /gene="pepN"
                     /locus_tag="VIG70_11195"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY8251166.1"
                     /translation="MKEPQPRAVHLKDYAPPAFRVDSVDLDVDIREDHALVRAKLEIR
                     RHAVGPLVLDGDELELVSVALNGKPVPYKVTEDKLTVAEVPDSFTLETVSRIVPQKNT
                     KLEGLYATKHGFVTQCEAEGFRRITWFVDRPDVMARYTTTVRADPKKYPVLLSNGNLV
                     ASGEGWAKFEDPFPKPSYLFALVAANLELLREGNLSIYVEPGNLDQAGWAMDCLKRAI
                     AWDEKRFGLKLDLEEYKIVAVGDFNSGAMENKGLNIFNTRYVLARADTATDVDYLNID
                     RVVAHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEYGADTYSRGVTRIQEVRSLRA
                     NQFPEDAGPMKHPVRPQSYMEIRNFYTMTVYEKGAEVVRMQHTLLGEEKFQAGMRLYF
                     KRHDGQAVTTDDFVQAMQDASGIDLQQFKRWYDVAGTPTVRCEGHYENGQFVLTAKQS
                     MSPPFHIPFAVKIGEHEQVLSLKKAEERFSFPAKEKPVPSLLREFSAPVILDYPYGED
                     DLLHLVARDDDPFNRWEAGQRLAATIILQRKGEPSARFVEAARKLLRDPDPAFAAEVL
                     SLPAETFLAEQMDVVDPDALHESRNALRRALARSLKEELLSTYQALQSKTPYSPDARS
                     IGRRALRNLCLGYLAELGLSTLAYEQFQRADNMTDSMAALATLANLDCPEREPALAAF
                     YERWKNEPLVVDKWLAVQAGSRRPATLDTVKRLLKHPAFDLKVPNRVYALVRTFAANH
                     VRFHAAGGGGYAFLVDQIVALNALNPQVAARMARSFDRWKRFDEKRQAHAKQQLERIR
                     DTEKLSKDVAEIVAKALA"
     gene            complement(5535..5873)
                     /locus_tag="VIG70_11200"
     CDS             complement(5535..5873)
                     /locus_tag="VIG70_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251167.1"
                     /translation="MRLAQVSSRLADLSEQYEVPEKLGRVSEKVHDGVSVASEAARKG
                     AAMAYRAALEHPRTAIGGVIIAAVLVGGALWYVFGDWRRPAAPRRRHATRVRAGAERR
                     RKPRQARAST"
     gene            5967..7715
                     /locus_tag="VIG70_11205"
     CDS             5967..7715
                     /locus_tag="VIG70_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8251168.1"
                     /translation="MAGSNWDQQYDLIVMGAGAAGMTTALVAAIEGLKPLLIEKTDRV
                     GGTTATSAGTIWIPGNRQSTAAGYQDSPEAAALYLDKLTGVPDASGLRSAFLSTGPDM
                     VEYLRAKSDVHFNPSGKHPDYRDMEGAAVYGRTLVPAMFDGRLLGKDFERVRPPIPEF
                     LLLGGMMAGKADLPPLINRFRSVGNFLYAGRLLLRYLADRLRYSRGTRIVMGNALVAR
                     LFYSLKKNRVPVMFDTTVMEVVSGAGRVAGVVVRSQGRTLRINTRKGLVIATGGYGHS
                     AKLRERFMPKPTPADTVACPANTGDGIELALRQGGTIGADAHGPGAFWAPVSRTRRAD
                     GSRGVFPHLLLDRAKPGLIAVNAAGKRFVNEADSYHDFVLGMYESHKSTPTIPAYLVC
                     EASFVARYGLGVVYPGTTNLKPFEKSGYLVVAPSLERLAAKLGIEPQALVATVARHNS
                     MARSGKDLDYGKGETELNRFNGDPLQAPNPCLRPIEQGPFVALAVWPAEIATSAGLQA
                     DVDGQLLDTKGIPIDGLYAVGNDMASIMLGTYPGPGTTLGPALTFGYRAAMHAAGKPV
                     GYSRRLFPSDPPSCGS"
     gene            complement(7667..9235)
                     /locus_tag="VIG70_11210"
     CDS             complement(7667..9235)
                     /locus_tag="VIG70_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8251169.1"
                     /translation="MSTPFDSLGLRAELLRAVAEQGYTQPTPIQLQAIPVVLAGRDLL
                     GAAQTGTGKTAAFALPILQNLGGKATSSHSPARHPVRALIVTPTRELAAQVEQDFRTY
                     GKHVQLKSSLVYGGVGMQPQVDALRRGVDILVATPGRLLDHVQQKTVNLGQVEILVLD
                     EADRMLDMGFINDIRKILALLPATRQNLLFSATFPDEIRKLAASFMKNPQTVEVARRN
                     TPAELVAQVQHPVSHDRKRELLAHLVKSNDWRQVLVFVKTKHGANRLAQQLERSGIEA
                     DAIHGNKAQNHRTRTLKRFKDNELRVLVATDIAARGLDIEALPHVVNYDLPHVAEDYV
                     HRIGRTGRAGAEGEAVSLVTHEDRPLMAAIEKLMNRKVDQRVIEGFEHGTRSQEPAED
                     RDNRGNRSQSPNSGRRQQQQPRQRQGQQRRPEQRHGQPKHGGNGHGGNHGGNRGQSPN
                     SQRFGGQRRGGRPGNGAAALPAQIAKEREAQIAEARARMAAEGEPVPAAPEPAKRSRY
                     EPQLGGSLGNKRRE"
     gene            complement(9407..10318)
                     /locus_tag="VIG70_11215"
     CDS             complement(9407..10318)
                     /locus_tag="VIG70_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY8251170.1"
                     /translation="MKSELSFEQAAGYAVCRFRAMASPCEVLVDSDDESMARRLGTLA
                     REEALRVERKFSRYRDDNIVHRINTAHGAPVAVDDETAALLDYADTCWRLSGGLFDIT
                     SGILRRAWRFQRGARVPAQAAVAALLPLIGWHHVRWAKPVIELPPGMEIDFGGLGKEY
                     AVDRCTRLLGVETTASLLVNFGGDLATTGARRDGHGWVIGVESPDSVSDVSAVTHAAR
                     LAFELSRGAVATSGDARRFVLDGGRRYGHILDPRTGWPVAGAPRSVTVVGNTCTEAGI
                     LATLAMLRGAGAEAWLENQGTRYWCLR"
     gene            complement(10315..11565)
                     /locus_tag="VIG70_11220"
     CDS             complement(10315..11565)
                     /locus_tag="VIG70_11220"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HEY8251171.1"
                     /translation="MAAATCGLLASAPAAHAQAPTTPRPAQRSPWEIESAILFYSEQD
                     RVTLIEPVIRMSRQLSEDEAIAIKLVVDSLTGSSPSGAVPSTTPQTFTSPSGNDTYRT
                     PANATPLDPTFLDTRVALSFDWTRPLGAERRIVYTGHASTEYDYTSIGLGATVSQDFN
                     KRSTTLTAGLSYNADKVEPVGGIPSGLTTQPAFPGVKTTAGASDGKSVLDALLGVTHV
                     FNRTTLMQFNYTYGRDSGYLTDPYKLVSVVDPATGIPVQTIYEKRPEDRARSTLYWRA
                     LKAFDRDVLNASYRYYWDDWGVKAHTVDLRYRRDLGGPYLEPHLRYSKQVQAANFYRP
                     FLRAGESEEFASADYRLAEMSTVTLGVKLAIPSKTGELSARLEYMLQTGEDHPAGAPG
                     QLAAQDLFPDTKAVIAQISYSFRW"
     gene            complement(11592..11810)
                     /locus_tag="VIG70_11225"
     CDS             complement(11592..11810)
                     /locus_tag="VIG70_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HEY8251172.1"
                     /translation="MNPRIMAALLCAALLAGCASENVEPWERDVLARKPMQLDAYPLD
                     TFIDEHIYFSKESASGGQGIGGGGCGCN"
     gene            complement(11807..12301)
                     /locus_tag="VIG70_11230"
     CDS             complement(11807..12301)
                     /locus_tag="VIG70_11230"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8251173.1"
                     /translation="MRRIRVLFVLALAAACALPSVTASAPISDFTFATDQGVLRLDSL
                     RGKVVYLDYWASWCVPCRQSFPWMNEMHSRYGDKGLVIVAVNVDRDAAEARSFLTQYP
                     AAFIVAYDPEGKTAKALALKGMPSSFVIDRQGQIVGTHVGFREAAKAGIAEQIKALLG
                     VPAR"
     gene            complement(12377..13243)
                     /locus_tag="VIG70_11235"
     CDS             complement(12377..13243)
                     /locus_tag="VIG70_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8251174.1"
                     /translation="MIAAPVLWSSAGVVTRHIQRAEPFEQVFWRSLFAFGFVFLALLL
                     QKAHPWRALRAAGWPGLASGLMWAIMFTAFLFALSLTTTANTLVVMSVSPLLTALFAS
                     VFLKDPVPLRTWLAAGAAAVGIAWMFGSSLEQHFLGMAIAFLIPVAAAINVVVLRASA
                     ANLDLIPAVMLGGALSCLLVLPFALPVSATPRDLALLAFLGVFQLGLPCMLLVIASRA
                     LLAPEIALLGLLEVVLGPLWAWLGAGEVPGSSTVLGGIVVLGALVANELYTARTASLR
                     KTSHLHSSIWRP"
     gene            complement(13249..18570)
                     /gene="uvrA"
                     /locus_tag="VIG70_11240"
     CDS             complement(13249..18570)
                     /gene="uvrA"
                     /locus_tag="VIG70_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY8251175.1"
                     /translation="MKHEQVPGDAIVIRGARQNNLKHLNLAIPTGELVVVTGVSGSGK
                     SSLVFDTLYAEGQRRYVETFSPYARQFLDRMDKPQVDAVEGIPPAIAIDQTNPVRTSR
                     STVGTMTELNDHLKLLFARGAKLHCRGCGKPVVRDTPTSIFDDLLKNGEARLVITFPV
                     PIPKNFTEKEVEDLLQRQGYTRLHSRSKTLLEVIQDRVRATPEDRARITEALESALKF
                     GQGKVNVYEGEKVLRFSSALHCAECDIQYAEPTPAMFSFNSPLGACESCRGFGRVIGV
                     DFGLVVPNETLSLKGGAVRTWQTKSFKECQDDLEKYAKKRKVPLDTPWHELDEKQKHW
                     VLEGEPEWVSWRKSWPGVWYGVRRFFKWLESKAYKMHIRVLLSKYRAYTECAACLGTR
                     LKPEGLLWRLDGKSIHDLVLQPISQTRTFFEKFGFDDEATDLLLKEIRTRLNYLCDVG
                     LGYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFALDEPSIGLHPRDMGRVIEVMK
                     RLRDAGNTLVVVEHDPQIMFAADRILDMGPGPGERGGEIVFFGPAEKLRAEKTLTAEY
                     LSGRRVAGEPRNPKPPREFIEVLGASQHNLKNIDVRIPLNRLVCVTGVSGSGKSTLIQ
                     DVLYPALRKSKGKPTEAPGAHRELRGAKKVSSVVMVDQSPIGKTTRSNPASYVGAFDE
                     IRKIFSKEKTSKERGYTPGTFSFNSGNGRCPTCSGCGFEHVEMQFLSDVYLRCPDCDG
                     RRYRDEILEVRIEGKSIADVLELTVAEAARFFRKSEEVQDRLAPLIDVGLEYLRLGQP
                     VPTLSGGEAQRLKLAGHLADTDDEADKLFLFDEPTTGLHFDDVAKLLRAFRQLLAAGH
                     SLVVIEHNLDVIRSSDWILDLGPEGGDAGGEIVCEGTPAEVARHETSFTGKALREYEE
                     AFKKPPVAKEPPGRYLGNSIRIHHAREHNLKNIDVEIPRGRFTVITGVSGSGKSTLAF
                     DILFNEGQRRYLESLNAYARQFVQAASRPDVDAIFGIPPTVAIEQRTSRGGRKSTVGT
                     LTEVHHFLRLLFVKLGTQHCPDCDVPIEPQSEEVIAARLLKQYRGQKISLLAPLVINR
                     KGVYTDLAKWALSKGYWHLRVDGKLLPTRPFPRIDRFKEHTIELPVGDIVVSAKAETQ
                     LRQLLSKTLEIGKGVVHVPAGKEVQIFSTKRACRSCGRSFPELDPRLFSYNSKHGWCA
                     ACYGTGLQIDDVEWSDERARTGAEDNVLDSWIEWLEIDEACPECEGRRLNREALAVRW
                     KGKNIAEYAGQPIDHLKLLFQDIPLAGRESEIARDIVAELNSRLGFLGEVGLGYLTLD
                     RSAPTLSGGEAQRIRLAAQLGSNLRGVCYILDEPTIGLHHRDNKILLDVLEKLEAKGN
                     SLVVVEHDEDTIRRAQHVIDLGPGAGKLGGAVVAQGDAEELMENPASLTGQFLRHPLR
                     HPLQPRRPVSKKNDSLEVHKASLHNLKNVDVRIPLGRLVAVTGVSGSGKSTLARDVLY
                     ESFMQKAAVGCKAIKGRERIERVLEVDQTPIGKTPRSCPATYVGFYDSIRKLFADTTE
                     ARVRGWGASRFSFNTRGGRCEICEGQGLKKIEMSFLPDVKVTCEGCGGARFNPETRQV
                     RLRGKTVGEVLGMSVDEAVGFFAAHPSIHRCLTLLQDVGLGYLTLGQQSPTLSGGEAQ
                     RIKLVYELAKRSEVKTLYVLDEPTVGLHMADVEKLIRVLHRLVDAGNTAVVIEHNLDV
                     IAEADWIVDLGPEGGDGGGKVVFQGIPDTMTAGHTAAALLPFLAQRR"
BASE COUNT         3019 a         6010 c         6406 g         3283 t
ORIGIN      
        1 ctgcatcgtc tgcatcattc ggcctcgatg ttcgcgtgct tcaccaccgc gcgccattcg
       61 gcgatctcgc gcttcaccat ctgtccgagc cgctcgggcg tggagctcat gccgtcggcg
      121 ccctgctcga gcagcttcgc cctgacgtcg gggcgcgcga ccacggtcgc gatctccttg
      181 ttcatgcgcg cgactacttc gtcgggcgtc ttcgccgggg cgaacacggc gtaccaggag
      241 tcgacctcga agtccttcag gccggtggcc tccgcgatgg tgggcacgtc gggcaggatc
      301 ggcgtgcgct tcgcggtgct caccgcgagc gggcgcaggc gcccggcgcg cacctgtccc
      361 atcgccgccg ggatcgacac gatgagcagc ggcacgtcgc ccgccatcgt cgccgcgacc
      421 gccgggccgc cgccgcggta cgggatgtgg accatgtgga tgccgagctt cagctcgagc
      481 agctcgcccg ccatgtggct cggcgagccg acgccgaccg aggcgaagtc gagcttgccg
      541 ggatggccct tcgtgtacgc gaccagctcc ttgaacgtct ggtacggcgt gcccggatgc
      601 accgcgaaga gctgcggcag cgagcagata gtggacaccg gccggaaatc gcgctcggtg
      661 tcgaacggca ggctcttgta gatcgcgggg ttgatcgagt gcgacgacag cgtgaagagc
      721 agcgtgtggc cgtcgggatt ggctttcgcc gcgacgccgg tgccaagcga gccgccggcg
      781 ccgccgcggt tctcgatcac gatcggctgc ccgagctgct tcgacagcgg ctcctgcacg
      841 atgcgcgcga tcacgtcggt tccgccgccg ggtggatagg gaacgacgaa ggtgatgggc
      901 ttcgtcggcc atgcgaaggc ggtgctcgcc gcaaccgcga gcgcaacgcc gaggaacgtt
      961 tttcgtgaac cgaggttcga tgtcatcact gctctcctga ctctcccccc tccggctgct
     1021 attgtagtcg catggagtcg atccaacgcg gggttgttgg gagcgcgcaa ctcgtcgtcg
     1081 gcgaggagca cacggcaccg cgcgtgggaa gtggaaaagt tcacgttctc gccacgccgg
     1141 tgatgatcaa tctcatagag gcggcggcgc ttgcggcgat cgagcacttg ctgccgccgg
     1201 gccaccagag cctcggcacc cggctcgacg tgcggcatat cgccgccacg ccggtcggaa
     1261 tgaaaatttt tgcaaacgcg accgtcgaga gcgtttcagg gcgcacggtc accttcaggg
     1321 tcgaagcgcg cgacgagaag gaactgatcg gcgatggcac gcacgaacgc gtggtggtga
     1381 acgtcgaaaa gttcgatgcc cgcgtgcagc gaaagctagc gcgcggttga gcgcagcccg
     1441 gcgatcagcc ggtcgatcgt cttctggata tcggcacgag cggcgctggg atcggccgag
     1501 gttgcgatca cgcgcgcgcc ggtggtaagc gcgccgagga tcacctgcgc cagggtctcg
     1561 gcgggctcgg gcagcgcgcc ggcgtcgcga ttcgcttcct ccaagcaccg cgcgaacgcc
     1621 gccacctcgt acttctgctc gaggtcgcac caggtcttcc agccgaggac caccggcgcc
     1681 tcgagtacca gcatgcgggc cagggcaggg tcgcggcagg cgtcgagata ggcgcggcac
     1741 atcgcgcgca gccgctccca cgggtcgcgg cgcccgcccg cggcggccgt ggcggcatcc
     1801 tcaaggcgct cgaccagctc acgctccatg tcctcgacca ccgagtggta gagctcgcgc
     1861 ttgttcgcga agtggtggta gagcgcgccc ttggtgacgc gcgcgcgctg caccagctcg
     1921 ttggtgttgg cgccggaata tccctttttg gcaaagatct tgcgcgcctc gcggcgcagc
     1981 gtcttctggg tctcgctgcc ctgcagcagc cgggcgtcat tgacttccat accgacggta
     2041 tgttatttac atacccgcgg tatgtcaaaa ccagaggaga ggaacgatgc cgaaatacgt
     2101 gatcgagcgc gagctgccgg gcgccggcaa gctgagccgc gacgagctga ggcaggtggc
     2161 gcagaagtcc aacggggtca tcgagaccct cgggcccaag atccagtggg tggagagctt
     2221 cgtggtcgac gacaagctgt attgcgtcta caacgcggct tcgcccaaga tcatcgagga
     2281 gcacgcgaaa tgcggcggct tcccagccaa ccgcatctcg caggtacggg cgatgatgga
     2341 cccgacgacg gccgaagcgt agtactaggg gtgaaacagc aggacggcga agaacgcggc
     2401 cagcccgatc gctggccgca gccacccgat ctcgcgccaa acgatgcggt tgcgcccctg
     2461 cgcgattgcg acgaacggca cgtgtgaggt caccgcggcg aagcgcggcc agtccggatt
     2521 cgctttcttt cgcgaatcca tcgacagggt tccgaccagg gcgacgacca gcaagccgcc
     2581 gaagaacacg agcgacttga ggtcgccgcg cgccacgatg tgcgccccgg cccacagcat
     2641 gattccccac atgatcgggt ggcgcgtgat gcggatcatg ccgcgcgccg gatcctcgct
     2701 tttgaggagc ttgtccgcgc cgaccatcgt gggattgcgc cagtagccgc aggcgatcag
     2761 gacaaaagcg accggcatcg cccagtaggg tgtattggga aacccactcc acagcaattc
     2821 tcggggggcc gaggcgtacg cccagaccat ccagccgagc gtcacgaagg ccaccagcga
     2881 atagacgccg cgatacggcc actcgcccat ggcgttgacc agcgccgggc gcagcggtgt
     2941 gctggtgacg aaatgcgtgg cgatgaatgc cacggtcgcg atcacgagca tcgtcatgct
     3001 ttttccccct tcagggggtc atgctagcgc tttggctacg atctcggcga cgtccttgga
     3061 gagcttctcc gtatcgcgga tgcgctcgag ctgttgcttc gcgtgcgcct gccttttctc
     3121 gtcgaagcgc ttccagcggt cgaacgagcg cgccatgcgg gccgcgacct gcgggttgag
     3181 cgcgttgagc gcgacgatct ggtcgacgag gaacgcgtag ccgccgccgc cggcggcgtg
     3241 gaagcgcacg tggttcgccg cgaaggtgcg cacgagcgcg tacacccggt tcggcacctt
     3301 gaggtcgaac gcggggtgct tcagcagccg ctttacggtg tccagggtcg cgggacgccg
     3361 cgagccggcc tggaccgcga gccacttgtc gaccaccagc ggctcgttct tccaccgctc
     3421 gtagaacgcg gctagcgcgg gctcgcgctc cgggcagtcg aggttcgcga gcgtggcgag
     3481 cgcagccatc gagtcggtca tgttgtcggc gcgctggaac tgctcgtagg cgagcgtcga
     3541 caggccgagc tccgccagat agccgaggca gaggttgcgc agcgcgcgcc ggccgatgga
     3601 ccgggcgtcg ggcgagtagg gggttttcga ttgcagcgcc tggtaggtgg agaggagctc
     3661 ctccttcagg gagcgcgcga gcgcccggcg cagcgcgttc ctcgattcgt gcagcgcgtc
     3721 gggatcgacc acgtccatct gctcggcgag gaaggtctcc gccggcaggc tcagcacctc
     3781 ggcggcgaat gcggggtcgg gatcgcgcag cagcttgcga gcggcttcta cgaagcgcgc
     3841 cgaaggctct cccttccgct gcaggatgat cgtcgcggcg aggcgctgcc cggcttccca
     3901 gcggttgaac ggatcgtcgt cgcgcgccac caggtggagc aggtcgtcct cgccgtacgg
     3961 atagtcgagg atcaccggcg cggagaactc gcgcagcagc gacggcaccg gcttctcctt
     4021 cgccgggaag ctgaagcgct cttccgcttt tttgagcgag agaacctgct cgtgctcgcc
     4081 gatcttcacc gcgaacggga tgtggaacgg cggactcatc gactgcttcg ccgtcaggac
     4141 gaactggccg ttctcgtagt gtccctcgca tcttacggtg ggcgtgcctg cgacgtcgta
     4201 ccagcgcttg aactgctgca ggtcgatccc cgatgcatcc tgcatcgcct gcacgaagtc
     4261 gtcggtggtg accgcttggc cgtcgtggcg cttgaagtac aggcgcattc cggcctggaa
     4321 cttctcctcg ccgagcagcg tgtgctgcat gcgcaccacc tcggcgccct tctcgtacac
     4381 cgtcatggtg tagaagttgc ggatctccat gtacgactgc ggccgcaccg ggtgcttcat
     4441 cggcccggcg tcctcgggaa actggttggc gcgcagcgag cgtacttcct ggatgcgcgt
     4501 gaccccgcgc gaataggtgt cggcgccgta ttcctggtcg cggaacaccg tcaggccttc
     4561 cttgagcgag agctggaacc agtcgcggca ggtgacgcgg ttgccggtcc agttgtggaa
     4621 gtactcgtgc gccaccacgc ggtcgatgtt caggtagtcc acgtcggtgg cagtgtcggc
     4681 gcgcgccagc acgtagcgcg tgttgaagat gttgaggccc ttgttttcca tggcgccgga
     4741 attgaagtcg ccgacagcga cgatcttgta ttcctcgagg tccagcttca ggccgaagcg
     4801 cttctcgtcc cacgcgatcg cgcgcttgag gcagtccatc gcccagcccg cctggtcgag
     4861 gttccccggc tcgacgtaaa tggagaggtt gccctcgcgc agcagctcga ggttggccgc
     4921 gaccagggca aagaggtacg aaggcttggg gaaggggtcc tcgaatttcg cccagccttc
     4981 gccggaagcg accaggttgc cgttggacag cagcaccggg tacttcttcg ggtcggcgcg
     5041 taccgtggtc gtatagcggg ccatcacatc gggccggtcg acgaaccagg tgatgcgccg
     5101 gaatccctcg gcctcgcatt gggtgacgaa gccgtgcttg gtggcgtaca ggccttcgag
     5161 cttggtgttc ttctgcggca cgatccgcga caccgtttcg agggtgaagg aatcgggcac
     5221 ctcggcgacc gtgagcttgt cttcggtcac cttgtacgga accggctttc cgttcagcgc
     5281 cacggaaacc agctcgagct cgtcgccgtc cagcaccagc gggccgaccg catggcgccg
     5341 gatctcgagc ttcgcgcgca ccagcgcgtg gtcttcgcga atgtcgacgt cgaggtcgac
     5401 cgagtcgacc cgaaatgccg gcggggcgta atccttgagg tggactgcgc ggggttgggg
     5461 ctctttcatc cccctattct agggcgcggc ggagcccgcg acgacgcgat atagcactga
     5521 ttaaaaaggg gaaactaggt ggaggcgcgc gcctgccgcg gcttgcggcg ccgctcggcg
     5581 cccgcccgga cgcgcgtcgc gtgccgcctg cggggcgccg caggccggcg ccagtcgccg
     5641 aacacgtacc acagggctcc gccgaccaga acggcggcga tgatgacgcc cccgatggca
     5701 gtcctggggt gctcaagggc ggcgcgatag gccatcgccg cgccctttcg ggcggcttcg
     5761 ctcgccacgc tgaccccgtc gtggaccttt tcggaaacgc ggcccagctt ctcggggact
     5821 tcgtactgct cggaaagatc agccaggcgg ctggaaacct gcgcgagtct catgcgtcct
     5881 ccagtgtcga tcacgctaaa aagagcagca atgcgcgtgc ccgcgtagtt ccaggccttc
     5941 cttcacatcg cttaagctcg aaagcgatgg ctggctcgaa ttgggatcaa caatatgacc
     6001 tgatcgtgat gggcgcgggc gccgcgggga tgacgacggc cctcgtggct gccatcgaag
     6061 gcctcaagcc gttgctgata gagaagaccg accgtgtggg cgggacgacg gcgacatcgg
     6121 cgggcacgat ctggatcccc ggcaaccgtc aaagcacggc cgccggctat caagacagcc
     6181 ccgaggcggc ggcgctttac ctggacaagc tgaccggcgt gcccgatgcc agcggcctgc
     6241 gctccgcatt cctctcgaca ggccctgaca tggtggagta cctccgcgcg aagagcgacg
     6301 tccacttcaa tccgagcggc aaacacccgg actaccgcga catggagggt gccgccgtgt
     6361 atggacgcac gctggttccg gcgatgttcg atgggcggtt gctgggcaag gacttcgagc
     6421 gcgtccggcc gcccattccc gagttcctgc tgctcggcgg gatgatggcc ggaaaggccg
     6481 acttgccgcc cctgatcaac cggttccgca gcgtcggcaa ttttctctat gccggcaggc
     6541 tgctgctacg ctacctcgcc gacaggctgc gctattcgcg cggcacccgc atcgtcatgg
     6601 gcaacgccct ggtcgcccgc ctgttctaca gcctgaagaa aaaccgcgtt ccggtgatgt
     6661 tcgacaccac tgtgatggaa gtcgtcagcg gcgcaggtcg cgtcgccggg gtcgtggtcc
     6721 ggtctcaagg ccggacactg cggatcaaca cgcgaaaagg gttggtgatc gccaccggcg
     6781 gctatggtca cagcgccaaa ctgcgcgagc ggttcatgcc caagccgacc ccggcggata
     6841 cggtagcctg cccggcaaat acgggcgatg ggatcgagct ggccctgcgg cagggcggca
     6901 cgattggcgc ggacgcgcat gggccgggag cgttctgggc gccggtatcg cgcactcgcc
     6961 gcgccgacgg cagtcggggc gtgtttcccc atttgctgct ggatcgcgcc aagcccggct
     7021 tgatcgccgt caacgcggcc ggcaagcggt tcgtcaacga ggcggactcg taccacgact
     7081 tcgtgctggg catgtacgag tcccacaaga gtacgcccac gatcccggct tatctggtct
     7141 gcgaggcttc tttcgtcgcg cgatacgggc tgggggtcgt ctaccccggc acgaccaact
     7201 tgaagccgtt cgagaagtcg ggctacctcg tcgtcgcgcc gtcattggag cggctcgccg
     7261 caaagctcgg gatcgagccg caagcgctgg tcgccaccgt cgcgcgccat aactcgatgg
     7321 ctcgcagcgg aaaggacctg gattacggca agggagagac cgagctcaac cggttcaacg
     7381 gcgatccgct gcaggcgccc aatccctgcc tgcgtcccat cgagcagggg ccgttcgtcg
     7441 cgctcgcggt ctggccggcc gagatcgcga cgagcgcggg tttgcaagcc gacgtcgatg
     7501 gccagttgct ggatacgaaa ggaattccca tagatgggct gtatgccgtg ggaaacgaca
     7561 tggcttccat catgctgggt acctatcccg gtccgggtac gacgctcggg cccgcgctga
     7621 ctttcggata ccgggcggcg atgcacgccg ccggcaagcc cgtcggctat tcccggcgct
     7681 tgtttccgag cgacccgccg agctgcggct cgtagcgcga gcgcttggcg ggctcgggcg
     7741 cggccgggac cggctcgcct tcggctgcca tccgtgcgcg cgcttccgcg atctgcgcct
     7801 cgcgttcctt tgcgatctgc gccggcagtg cggctgcgcc attaccggga cgaccgccgc
     7861 ggcgctggcc gccgaagcgc tgcgagttcg ggctctgacc ccgatttccg ccgtgatttc
     7921 cgccgtgccc attgccacca tgtttcggct ggccgtggcg ctgctcgggg cggcgctgct
     7981 ggccttgccg ctggcgcggc tgttgctgct gccggcgccc tgaattcggg ctctgggacc
     8041 gatttccgcg gttgtcccga tcttcagccg gttcttggct gcgggtgccg tgctcgaagc
     8101 cttcgatcac gcgctggtcg accttgcggt tcatcagctt ctcgatcgcg gccatgaggg
     8161 gacggtcttc gtgcgtcacc agcgatacgg cttcgccttc ggcgccggcg cggccggtgc
     8221 ggccgatgcg gtggacgtag tcctcggcga cgtgcggcag gtcgtagttg acgacgtgcg
     8281 gcagggcctc gatgtcgagg ccgcgcgccg cgatgtcggt cgccacaagg acgcgcagct
     8341 cgttgtcctt gaagcgcttg agggtgcggg tgcggtggtt ctgggctttg ttgccgtgga
     8401 tcgcgtcggc ctcgatgccg gagcgctcga gctgctgcgc gaggcggttg gcgccgtgct
     8461 tggttttcac gaacaccagg acctggcgcc agtcgttcga cttgaccagg tgggcgagga
     8521 gctcgcgctt gcggtcgtgg ctcaccgggt gctggacctg cgcgacgagc tcggccgggg
     8581 tgttgcgccg ggcgacttcc acggtctgcg ggttcttcat gaaggacgcc gcgagcttgc
     8641 ggatctcgtc ggggaaggta gcggagaaca gcaggttctg ccgcgtcgcc ggcagcaggg
     8701 cgaggatctt gcggatgtcg ttgatgaagc ccatgtcgag catgcggtcg gcttcgtcca
     8761 gcaccaggat ctcgacctgg ccgaggttca cggtcttctg ctggacgtgg tcgagcaggc
     8821 ggccgggggt ggcgacgagg atgtcgacgc cgcggcgcag cgcgtcgacc tgcggctgca
     8881 tgccgacgcc gccgtagacg aggctcgatt tcagttgaac gtgcttgccg taggtgcgga
     8941 agtcctgctc gacctgcgcc gcgagctcgc gcgtcggcgt gacgatgagc gcacgcaccg
     9001 ggtggcgggc gggggaatgg gacgaggtcg ctttccctcc tagattttgg aggatgggca
     9061 aggcaaaagc tgcggtcttg ccggtgccgg tctgcgcggc gccgagcagg tcgcgccccg
     9121 cgaggacgac cgggatggct tgaagctgga tcggggtggg ttgggtatag ccctgctccg
     9181 caacagcgcg gagcagctcg gcgcgaaggc cgagtgaatc gaacggcgtg ctaatggaaa
     9241 atctcctgag atcggcctgt gaagggctag ccttcgaccg gttcaggcag atgcttttta
     9301 ggcttgtttt tgagcggtcc aggatggacc gctaagaacg gactgagacg ctaaccggat
     9361 gtgcgcaaca gcaacctgga gctcattgta ccagcaaaca gctcgctcag cgtaagcacc
     9421 agtagcgcgt gccctgattt tccagccacg cttcagcgcc ggcgccgcgc agcatggcca
     9481 gcgtggcgag aattccggcc tcggtgcagg tgttgcccac gacagtgacc gaacgcggcg
     9541 cgcccgccac tggccagccg gtgcgcggat cgaggatgtg gccgtagcgc ctgccgccgt
     9601 ccaggacgaa gcggcgcgca tcaccgctgg tggcaacggc gccgcggctg agctcgaatg
     9661 caagccgcgc cgcgtgcgta accgcgctca cgtcgctgac gctgtcgggg ctctccacgc
     9721 cgatcaccca gccatggccg tcgcgccgcg cgccggtggt ggcaagatcg ccaccgaagt
     9781 tgaccagcaa gctcgccgtc gtctcgacgc cgagcaggcg cgtgcagcga tcgacagcgt
     9841 attccttgcc cagaccgccg aaatcgatct ccatgccggg aggcaactcg atgaccggtt
     9901 tcgcccagcg cacgtgatgc catccgatca gcgggagtag cgccgccacg gccgcctgtg
     9961 ccggcacgcg agcgccgcgc tggaagcgcc acgcgcgccg caatatgcca gaggtgatgt
    10021 cgaacaatcc gccgctcagc cgccagcaag tgtcggcgta gtcgagcagc gcggctgtct
    10081 cgtcgtccac agccacgggc gccccgtgcg ccgtgttgat gcgatgcacg atgttgtcgt
    10141 cgcgatagcg gctgaacttg cgctccaccc gcaacgcctc ctcgcgcgcc agggtgccca
    10201 ggcggcgcgc catcgactcg tcgtcggaat cgaccaggac ctcgcacggg ctcgccatcg
    10261 cccggaagcg gcagaccgcg tagcccgcgg cctgctcgaa gctcaactca gacttcacca
    10321 gcggaacgag tagctgattt gggcgatgac cgccttggtg tccgggaaca gatcctgggc
    10381 agccagctgg ccgggcgcgc cggcagggtg gtcctccccg gtctgcagca tgtactccag
    10441 gcgtgcgctc agctcgccgg ttttcgaagg gatcgccaac ttcacgccca gggtgacggt
    10501 gctcatctcc gccagccggt agtccgcgct tgcgaattcc tcggattcgc cggcgcgcag
    10561 aaacggccgg tagaaattcg ccgcctgcac ttgcttcgaa taccgcaggt gcggctccag
    10621 gtaggggccg cccagatccc ggcggtagcg caaatccacg gtgtgggcct tgacgcccca
    10681 gtcatcccaa tagtaccggt acgacgcgtt gagcacgtcc cggtcgaacg ccttcagcgc
    10741 gcgccaatac agcgtgctgc gagcgcgatc ctccgggcgt ttctcgtaga tcgtctgcac
    10801 tggaatgccg gtcgcggggt cgaccacgga gaccagcttg tacggatcgg tgaggtagcc
    10861 ggaatcgcgg ccgtaggtgt aattgaattg catcagcgtg gtgcggttga agacgtgcgt
    10921 gacgccgagc agcgcgtcga gcacgctctt gccgtcgctg gcgcctgccg tcgtcttcac
    10981 tcccggaaac gcgggctgtg tcgtgagccc cgacggtatg ccgcccaccg gctcgacctt
    11041 gtcggcgttg tacgagagtc cggcggtgag cgtcgtgctg cgcttgttga agtcctgcga
    11101 caccgtcgcg ccgaggccga tcgaggtgta gtcgtactcc gtggaggcgt ggccggtgta
    11161 gacgatgcgc cgctcggctc ccaggggccg ggtccaatcg aaagagagcg ccacgcgcgt
    11221 gtcgaggaaa gtcggatcga gcggcgtcgc gttggccggc gtgcggtacg tgtcgttgcc
    11281 cgaaggcgag gtgaaggttt gcggcgtcgt agagggtacc gcgccgctcg ggctcgagcc
    11341 ggtgagcgaa tccactacca gcttgatggc gatggcttcg tcctcagaga gctgcctgct
    11401 catgcggatt accggctcga tgagtgtgac gcgatcctgc tcgctgtaaa acaggatcgc
    11461 agactcgatc tcccacgggc ttcgctgcgc cggccgcggc gtggttggcg cctgggcgtg
    11521 ggccgccggc gcgctcgcga gcaggccgca ggtggcggcg gccaatgcct gcaggatgga
    11581 tgccggcttg ttcagttgca gccgcagccc ccgccgccga tgccttgacc gccgctcgcc
    11641 gattccttcg agaagtagat gtgctcgtcg atgaaggtgt cgagcgggta ggcatcgagc
    11701 tgcatgggct tccgcgcaag cacatcacgc tcccatggct ccacgttctc gctcgcgcag
    11761 ccagcgagca gcgcggcgca aagcagcgct gccatgatgc gagggttcac cgcgccggaa
    11821 ctcccagcaa tgccttgatt tgttccgcga tgccggcctt ggcggcttcg cgaaagccaa
    11881 catgcgttcc gacgatctgt ccttggcggt cgatcacgaa cgaactcggc atgcctttca
    11941 gcgccagcgc cttcgcggtc ttgccttctg ggtcgtaggc gacgataaag gcggccggat
    12001 attgcgtcag gaagcttcgc gcctcggcgg cgtctcggtc tacgttcacg gcgacaatga
    12061 ccagcccctt gtcgccgtac cgcgagtgca tctcgttcat ccacgggaac gactggcggc
    12121 aaggcacgca ccaggacgcc cagtaatcga gatacacgac cttgccgcgc aaactgtcga
    12181 ggcgcagcac tccctgatcc gtcgcgaagg tgaaatcgga aatcggcgcc gaggctgtta
    12241 cgctcggcag cgcgcaggcg gcggcgaggg ccagcacaaa cagaacgcgg attcgtcgca
    12301 tccgctcgga taataccctt gagcacggcc ttttttgaga tccggtttta ggatgggaag
    12361 gcgtggttgc gggctgctat ggccgccaga tagaagaatg aagatgtgaa gtttttctca
    12421 gtgaagctgt ccgcgccgtg tagagctcgt tcgcgactag cgcgccaagc accacgattc
    12481 cgccgagcac ggtcgagctg ccgggcactt cgccggcgcc gagccacgcc cacagcggcc
    12541 caaggacgac ttcaagcaat cccagcaagg caatttccgg agcgagcagt gcgcggctcg
    12601 cgatcacgag cagcatgcaa ggcagcccga gctgaaagac gccgaggaag gccagcaggg
    12661 caaggtccct tggagtcgca gaaacgggca gggcaaaagg caggacgagc aggcacgaca
    12721 gcgcgccgcc cagcatcacg gccgggatga gatcaaggtt cgccgcgctc gcgcgcagga
    12781 cgacgacgtt gatcgccgcc gcgaccggga tcaggaaggc gatcgccatg ccgaggaagt
    12841 gttgctccaa tgaagagccg aacatccacg cgatgccgac cgcggcggcg cccgcggcga
    12901 gccacgtgcg caatggcacc gggtctttca ggaagacgct cgcgaagagc gcggtcagga
    12961 gcggactgac gctcatgacg acgagagtgt tggcggtagt ggtgagcgac agggcgaaca
    13021 ggaacgcggt gaacatgatc gcccacatca ggccggaggc gaggcccggc cagccggcgg
    13081 cgcgcagcgc tctccacggg tgggcttttt gcagaagtag agccaggaaa acaaaaccga
    13141 aagcgaacag gctcctccag aacacctgct cgaaaggctc ggcgcgctgg atgtggcgcg
    13201 tcaccacacc ggcgctcgac cagagcaccg gcgcggcgat catcagggct accgccgttg
    13261 cgcgagaaat gggagcagag cggcggcagt atggccggcc gtcatggtgt cggggatccc
    13321 ctggaacacg actttgccgc cgccgtcgcc gccttccggg ccgaggtcga cgatccagtc
    13381 ggcttccgcg atcacgtcga gattgtgctc gattactacg gccgtgttgc ccgcgtccac
    13441 gaggcggtgg agcacgcgga tcagcttctc tacgtcggcc atgtgcaggc cgacggtcgg
    13501 ctcatccaat acatatagtg tcttgacctc gcttcgcttc gccaattcgt agacgagctt
    13561 gatgcgttgc gcttcgccgc cggacaaggt gggactctgc tgcccgaggg tgaggtagcc
    13621 gaggccgacg tcctgcagca gggtcaggca gcggtggatc gagggatgcg cggcgaagaa
    13681 cccgaccgcc tcgtcgacgc tcatgccgag cacctcgccg accgtcttgc cgcgcagccg
    13741 cacctgccgc gtttcggggt tgaagcgcgc gccgccgcag ccttcgcacg tcaccttcac
    13801 gtcgggcagg aaggacatct cgatcttctt caggccctgg ccttcgcaga tctcgcagcg
    13861 cccgcccctg gtgttgaagg agaagcggct cgcgccccag ccgcgcacgc gcgcctcggt
    13921 ggtatcggcg aagagcttgc ggatcgagtc gtagaagccg acgtacgtcg ccggacagga
    13981 gcgcggcgtc ttgccgatcg gcgtctggtc gacctcgagc acgcgctcga tgcgctcgcg
    14041 gcccttgatc gccttgcaac cgacggcggc tttctgcatg aaggattcat agagcacgtc
    14101 gcgcgcgagg gtcgacttgc ccgaacctga aactcccgtg accgcgacca ggcggccgag
    14161 cgggatgcgc acgtcgacgt ttttcaggtt gtgcaggctt gccttgtgca cctccagcga
    14221 atcatttttc ttcgaaaccg gccgccgggg ctggagcggg tgacgcaggg ggtgacgcag
    14281 aaactgcccc gtcagcgagg cggggttctc catgagctcc tccgcgtcgc cctgcgcgac
    14341 caccgcgccg ccgagcttgc cggcgcccgg gccgaggtca atgacgtgct gcgcgcggcg
    14401 gatggtgtcc tcgtcgtgct cgaccaccac cagcgagttg cccttggctt cgagcttctc
    14461 gagcacgtcg agcaggatct tgttgtcgcg gtggtgcagg ccgatcgtcg gctcgtcgag
    14521 gatgtagcac acgccgcgca ggttggagcc gagctgcgcc gcgagccgga tgcgctgcgc
    14581 ctcgccgccg gaaagggtcg gcgccgagcg gtcgagcgtc aggtagccca ggcccacctc
    14641 gccgaggaag ccgaggcgcg agttcagctc ggccacgatg tcacgggcga tctcgctttc
    14701 acgccccgcc aatggaatgt cttgaaaaag caacttcaaa tggtcgatgg gctgccccgc
    14761 gtactcggcg atgttttttc ccttccagcg tacggcgagc gcctcgcggt tcaggcgccg
    14821 gccctcgcat tccgggcagg cttcgtcgat ctcgagccac tcgatccagg aatcgagcac
    14881 gttgtcctcg gcgccggtgc gcgcgcgctc gtcgctccac tcgacgtcgt cgatctgcag
    14941 gccggtgccg tagcaggcgg cgcaccagcc gtgcttggag ttgtaggaga aaaggcgcgg
    15001 gtcgagctcg ggaaagctgc ggccgcacga gcggcaggcg cgcttggtgg aaaagatctg
    15061 gacttccttt ccggccggta cgtgcactac gcctttgccg atttcgagcg tcttggagag
    15121 gagttgacgc aactgggtct ccgccttcgc ggagacgaca atatccccta cgggcaattc
    15181 gatcgtgtgc tctttgaacc ggtcgatgcg cgggaagggg cgcgtcggca ggagcttgcc
    15241 gtcgacgcgc aggtgccagt agcccttcga cagcgcccac ttcgcgagat cggtatagac
    15301 gcccttgcgg ttgatgacga gcggcgccag cagggagatt ttctgtcccc ggtactgctt
    15361 cagcagccgg gccgcgatga cttcctcgct ctgcggctcg atcggcacgt cgcagtcggg
    15421 gcagtgctgc gtacccagct tcacgaagag caggcgcagg aagtgatgca cctcagtcag
    15481 cgtgccgacg gtggacttgc gcccgccgcg gctggtgcgc tgctcgatcg ccaccgtcgg
    15541 cgggatgccg aagatcgcgt cgacgtcggg gcgcgaggcg gcctggacga actggcgcgc
    15601 gtaggcgttc agcgattcga ggtagcggcg ctggccctcg ttgaacagga tgtcgaaggc
    15661 gagggtcgac ttgcccgacc cggaaacgcc ggtgatcacc gtgaagcggc cgcgcgggat
    15721 ctcgacgtcg atgttcttga ggttgtgctc gcgcgcgtgg tggatgcgga tcgagttgcc
    15781 gagatagcgg cccggcggct ccttcgccac gggcggcttc ttgaacgctt cttcgtactc
    15841 ccgcagggct tttcccgtga acgaagtctc gtgcctcgcg acttccgccg gcgttccttc
    15901 gcacacgatc tctccgcccg cgtcgccgcc ttccggcccg aggtcgagga tccagtcgga
    15961 cgagcggatc acgtccaggt tgtgctcgat caccaccagc gaatggcccg cggcgaggag
    16021 ctggcggaaa gcgcgcagta gcttcgcgac gtcgtcgaag tgcagcccgg tggtgggctc
    16081 gtcgaagaga aagagtttgt ccgcctcgtc gtcggtgtcg gcgaggtgcc ccgcgagctt
    16141 caggcgctgc gcctcgccgc cggacaaggt ggggaccggt tgcccaagac gcaggtattc
    16201 caggccgacg tcgatcagcg gtgcgagacg gtcctgcacc tcctcgctct tgcggaagaa
    16261 gcgcgcggct tcggccacgg tcagctcgag cacgtcggcg atcgacttgc cttcgatgcg
    16321 tacctcgagg atctcgtcgc ggtagcgccg gccgtcgcag tcggggcagc gcaggtacac
    16381 gtccgagagg aattgcattt cgacgtgctc gaagccgcag cccgagcagg tcgggcagcg
    16441 gccgttgccc gagttgaagc tgaacgtgcc gggggtgtag ccgcgctcct tcgaggtttt
    16501 ttcctttgaa aagatcttcc tgatttcgtc aaaggcgccg acatagctcg ccgggttcga
    16561 gcgcgtggtc ttgccgatcg gcgactggtc gaccatcacg acgctgctca cttttttcgc
    16621 gccgcgcagc tcgcgatgcg cgcccggcgc ctcggtcggc ttgcctttcg acttgcgcag
    16681 cgccgggtag agcacatcct gaatcagcgt ggacttgccc gagccggaca cgcctgttac
    16741 gcacaccagc ctgttgagcg ggatgcgcac gtcgatgttc ttcaggttgt gctgcgacgc
    16801 gccgaggact tcgatgaatt cccggggagg cttggggttc cggggctcgc cggcgacgcg
    16861 ccggccggag agatattccg cggtcagggt cttttcagcc cgcaattttt ccgcgggccc
    16921 gaagaagacg atctcacccc cgcgctcccc cggtcctggc cccatgtcga ggatgcggtc
    16981 ggcggcgaac atgatctgcg ggtcgtgctc gaccacgacg agcgtgttgc cggcgtcgcg
    17041 cagccgcttc atcacctcga tcacccggcc catgtcgcgc ggatgcaggc cgatcgaggg
    17101 ctcgtcgagc gcgaacagcg tattcacgag cgaggtgccg agtgcggtgg tcaggttgat
    17161 gcgctgcacc tcgccgccgg aaagggtgcg cgactggcgg tcgagcgtga gatagcccag
    17221 gccgacgtcg cacaaatagt tcaggcgcgt gcggatctcc ttcagcagga ggtcggtcgc
    17281 ctcgtcgtcg aaaccgaact tttcgaagaa cgtccgcgtt tgcgagatcg gctgcagcac
    17341 gagatcgtgg atcgacttgc cgtcgagcct ccacagcaga ccctcgggct tgagccgggt
    17401 acccaggcag gcggcgcact cggtgtaggc gcggtacttc gagagcagca cccggatgtg
    17461 catcttgtag gccttgctct ccagccactt gaagaagcga cgcacgccgt accacacgcc
    17521 gggccaggac ttgcgccagc tcacccactc cggctcgcct tcgaggaccc agtgcttctg
    17581 tttctcgtcc aattcatgcc acggcgtgtc gagcggaacc ttccgtttct tcgcgtactt
    17641 ctcgagatcg tcctggcact ccttgaagct cttcgtttgc caggtccgga ccgcgccgcc
    17701 cttcaggctg agcgtttcgt tgggaacgac gagcccaaaa tcgacgccga tcacgcgccc
    17761 gaagccgcgg caggactcgc aggcgccgag cggcgagttg aacgagaaca tcgccggcgt
    17821 cggctcggcg tactggatgt cgcactcggc gcagtgcagg gcagacgaga agcggagcac
    17881 cttctcgccc tcgtagacgt tcaccttgcc ctggccgaac ttgagcgccg attcgagggc
    17941 ttcggtgata cgcgcccggt cttccggtgt cgcgcggacc cggtcctgga tcacctcgag
    18001 gagcgtcttc gagcgtgagt ggaggcgggt gtagccctgg cgctgcagga ggtcctcgac
    18061 ctctttctcg gtgaagttct tcgggatcgg caccggaaac gtgatgacca ggcgcgcctc
    18121 gccgtttttc agcaggtcgt caaaaatcga cgtgggcgtg tccctgacca cgggcttgcc
    18181 gcagccgcgg cagtggagct tcgcccccct ggcgaagagg agcttcaggt ggtcgttcag
    18241 ctcggtcatc gtcccgaccg tggagcgcga ggtgcgcacc gggttggtct ggtcgatggc
    18301 gatggcgggc gggatgccct cgaccgcgtc gacctggggc ttgtccatgc ggtcgaggaa
    18361 ctggcgtgcg tacgggctga aggtctcgac gtagcgccgc tgcccctcgg cgtacagcgt
    18421 gtcgaagacg agcgaggact tccccgagcc cgaaacgccg gtcaccacga ccagctcgcc
    18481 ggtcgggatc gcgaggttga ggtgctttag gttgttctga cgggcgccgc ggatgacaat
    18541 ggcgtcgccc gggacttgct catgcttcac tggatcgcat tttatctgct gctggccgct
    18601 tttggcctcg cgtcggcgca aagttcgcag ccgcaggtcc tcgtcgtgcc gctcgaaggc
    18661 gcgatcggtc cggcgagcgc ggatttcgtc ggccgcgcga tcaagcgcgc ggcgaaag
//
LOCUS       DATBSZ010000173        22767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1872419, whole genome shotgun sequence.
ACCESSION   DATBSZ010000173 DATBSZ010000000
VERSION     DATBSZ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 22767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22767
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872419"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2018)
                     /locus_tag="VIG70_11245"
     CDS             complement(<1..2018)
                     /locus_tag="VIG70_11245"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8251176.1"
                     /translation="MSVAQSTTIGRPVLRKEDERLLRGAGCYADDVNLPGQVYACMVR
                     SPHAHAKILSIKAGISGVIAVLTGKDAAADGLQPIPTRPVTVNPHEVKLQNRDGSPFH
                     IPPYPALPVDEVRFVGQAVAMVIAGTPMAAKDGADAVEVEYEVLPPHATTLVDADVGN
                     DVEKAFQSAAHVVRLDTAINRITGVPLEPRAVVAAFADGRYTIHAPTGSAQRFRADAA
                     AALGVPESAVRFVAREVGGNYGTRNYLCPEIPLVAWAAKRLGKPVKWRAERQECFLSD
                     DHARALSVQAELALDKDGNFLAFRSANTSDVGAYPFSYIPLTKGVGVATSVYHVPCAA
                     FRARAVTSNTMATSPYRAAGRPEVMFVIERLIDLAARKHGFDRAELRARNLIRSFPYR
                     NNQGLVYDSGDYPAVQRRALELAEWSSFEKRRSASRSRGKLRGIGMGHYIELNTGAPR
                     ERAQITVKRDGTVDVVLGTISSGQGHETSFAQLVSDWLGVDMAQVRLHAGDTDVSSIG
                     GGTHSGRSMRMGAVVIDVAVKRIVEQGRQAASEKLEAAVADIEFANGEFRVKGTDRRI
                     GLFDLAPLAGEHDNTDALPSYAFGCAVCEVEIDPETGVVEVVRYTSVDDVGRAVNPLI
                     IHGQTHGGIVAGVGQALMEHIVYDADGQLQTASFMDYAMPRADQFPFFT"
     gene            2056..3057
                     /locus_tag="VIG70_11250"
     CDS             2056..3057
                     /locus_tag="VIG70_11250"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251177.1"
                     /translation="MQRIVLGLVLAASAAGAVAQTKLPFRLNWTASGEHAAYFVARDK
                     GFYGDEGLEVEILDGSGSTTVLQLAANASNPVVQADASTMMRGIVNGMPVKAVAVPLQ
                     ISPSAFIYRADAPRPTKVSEVKGMRIAMTAGDSSMTVFTAFLGKIGLKLEDVQIITVA
                     STAAKDQAMLNKQADALIGFFMDQGVRLEPQTGVKIGYTRLYDLGQVTALSSAIVANS
                     EWLKDAKNQDTLRRFLRASQRGWQYAFDNRREAAEIFIKAKPAQQLNIAQGTLEGAMT
                     LVRTERSKGMPMLASAPQDWKDTQELLATYAKVKPMPDLDAYYTNAYLSEAPYLPKR"
     gene            3054..3530
                     /locus_tag="VIG70_11255"
     CDS             3054..3530
                     /locus_tag="VIG70_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY8251178.1"
                     /translation="MILEQLKKAGIDLVASLPDQWLGDLIKQCEADPAITHIRLARED
                     DGVGICAGAWLGGRKAALVCQSSGVLLAVNALGGIAFHHQIPLLVIAAHRGHYDDNQY
                     YQMYKGRICEPVLKAMGLPHWVIDGPEGLPMIGDAARQAYLNRLPVVVLLRKKALQ"
     gene            3527..4084
                     /locus_tag="VIG70_11260"
     CDS             3527..4084
                     /locus_tag="VIG70_11260"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY8251179.1"
                     /translation="MKRLALAKMVKQRLRKEDLVVCGLGATEAAFKEAAPANPTYYAS
                     DPMGIWASLALGLALARPKNRVTLLAGDGDLLMNLQVLATIATAPVPNYRIVVFHNGA
                     YSSTGGQPLAGKLSFSGVAKSAGFEWAHEARTEDETAKCLDELYARPGPALLDVWLED
                     EPCPTAPPGPLSQVEVRTLFMRDIS"
     gene            complement(4065..5381)
                     /locus_tag="VIG70_11265"
     CDS             complement(4065..5381)
                     /locus_tag="VIG70_11265"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY8251180.1"
                     /translation="MREFLQRVRGSGEIHEIRRAVDPVKELGAVLNACERAGKAAYFH
                     KVGNHDIPVTGALLSSPSRIALALGCTEPEVRARMAAATEKPLGFEIQKGKPQCQEIV
                     VEKPDLSKLPIPTHSPLDAGPFITAGVVIARDPETGRHNLSYNRMQIYGADRAGISMN
                     IWRHIKAFFDKIEPQGKNLPFCVALGPDPVVMMAAAFRYAGDEYEVAGALRQAPIPVV
                     RALTCDVLVPASAEIILEGEVLAGERQMEGPMAEFTGHYSGADPKHVARITRITHRKN
                     PIFQTMNGGGYEHVSVGNMITREPLLERFVRHISPRVKAVHLPPYSGGFTALIALDSP
                     EPGEAKNVALAAMAAHVNFKTVIVVDADVDIFDPADVMWALSTRARWDRGFSAVHGAH
                     TNELDPSSDEHGIVAKVIIDATLPADKRKRYLKVVYPPVDLRKYLA"
     gene            complement(5387..6760)
                     /locus_tag="VIG70_11270"
     CDS             complement(5387..6760)
                     /locus_tag="VIG70_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8251181.1"
                     /translation="MKADFLEALGNIPHSRDAAALRVKSADFSWFSPIMSAALAGRQA
                     EVVVSPRDEQEVVTIASACARHRVPLVARGGGTGNFGQAVPLAGGAVVEMVGLDRLVF
                     QKGNVARAQAGIRLGALDEQLRKGGRELRMHPSTKKLATLGGFVGGGHAGVGTVNWGI
                     LRDRGNLLGLKIITIEEKPRVLELRGDDVNLVHHAYGVNGLVTEVELPLAPAYEWLDT
                     IVAFDDFERAAAFGMALATADGVLKKLVSVHAWPIPGFFVPLKPHLPEGRAAALLMIG
                     AETREAFEALVRDFGGSVTMCVPQANIYEYTWGHTTMHALRADRSLTYLVCIFPKDGT
                     LGAIGRVHRKLGAEVMLHLEFKRFAGYMTAEGVPLFRYQGFEQMTRLYKAFEAEGVRI
                     ANAHTFLLQNGGMKDLDAAQIDFKRAVDPHLLMNPGKVAGLDDLPGGLSGAALEAAQL
                     PASGWAY"
     gene            complement(6750..7535)
                     /locus_tag="VIG70_11275"
     CDS             complement(6750..7535)
                     /locus_tag="VIG70_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8251182.1"
                     /translation="MKAALTRIIPPVLILVIGLAAWEIAVRAYKVPVYIVPGPIVIVQ
                     ALVRDWATLYPSLIVTVLITLSALTAATILGVLIAVLFAQSKWIEMSFFPYAVILQVT
                     PVVAIAPLIIIWVKDTATALLICAWIVAFFPIVSNTTLGLNSTDHNLLNLLQLYGASR
                     RQVFWYLRLPSALPYFLGGLRISGGLALIGAVVAEFVAGSGGIQSGLAYRILEAGYQL
                     QIPRMFAALFMITGTGILIFVILTAVNHFALRRWHESAVRREG"
     gene            complement(7532..8278)
                     /locus_tag="VIG70_11280"
     CDS             complement(7532..8278)
                     /locus_tag="VIG70_11280"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251183.1"
                     /translation="MRADAPLLALRGVDKVYSNGIVALQGLDLTIREGEFVSLLGPSG
                     CGKSTVLRLIAGLGELSGGLLEWSSVQHEIGFVFQDPTLMPWATVFNNIYLPLRVRGV
                     KKTDATPRVERAMEQVGLAGFGSAYPRELSGGMRMRVSIARALVAQPKILLMDEPFAA
                     LDEITRFRLNDDLLELWASQRWTVVFVTHSVFESVYLSTRILVMSSRPGRVRSEVLVE
                     TKRDASFRTSAAYADQCRTVSARLAEAMQA"
     gene            complement(8278..9207)
                     /locus_tag="VIG70_11285"
     CDS             complement(8278..9207)
                     /locus_tag="VIG70_11285"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251184.1"
                     /translation="MKLTFQSNWRAQAEHGGYYQAVATGIYAKHGLDVTVRPGGPQIN
                     NPQLLVASAIDFSMGSDGFNGLNFVKNEIPLVVVASLFQKDPRVLIAHPGQGNDSLAA
                     MKGKPILIASASRNNYWQFLRAKYGYTDDQIRPYTFNMAPFLADKSTIQQGFLTSEPL
                     KAQQAGVKPVIHLLADNGYVSYATTLETRSQLVKERPDVVQRFVNATIEGWYSYMYGD
                     RTAANALIKKDNPEMGDDQLAYSHAKMLEYGIVDSGDALKLGIGAMTDARWREFADSM
                     VKAGVYPADIDVKKAYTLQFVNKAHGMNLKPKN"
     gene            complement(9311..9796)
                     /locus_tag="VIG70_11290"
     CDS             complement(9311..9796)
                     /locus_tag="VIG70_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2165 domain-containing protein"
                     /protein_id="HEY8251185.1"
                     /translation="MNIIQLCQALFVALIGLFALLVAYNNLRDPQSNLRLVEHVLGMD
                     SVFPDTRLRERAITSPAAHRIAFWLIVAAEFATAALCLIGAAILLRVHGSGADAFHAA
                     KGVAFAGLGLGFALWFGGFMVIGGQWFASWQSKQWSGRESAFMFYSAIGIAFLALLHQ
                     A"
     gene            complement(9793..10983)
                     /locus_tag="VIG70_11295"
     CDS             complement(9793..10983)
                     /locus_tag="VIG70_11295"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8251186.1"
                     /translation="MSALLLRGKPADILIGASGRIERVAPGIEAGPGVKVVELNGRLA
                     VPGLVDAHQHLDKTRLRKLPNPDGTLQGAIGVFSRYAVDMSGADVAARAERTMAACLE
                     RGTVAIRSHANVDPQARTRGVEALVGVRERWRERLRLQVVAFMTAGATKAGVPAREWL
                     DEAMRLGADVVGGTPTYADEPLKFLDMLFDAAERHGKPIDLHLDEHLDAARIQFQAVI
                     ERTRAHGMAGKVVASHCSALSALEAGAAMKVIDGFASAGIGVVTLPAANLFLQGRAAT
                     KLTPRGLTRVLELQAAGVPVAAASDNIQDPFVPSGSGDLLEIARWTLLAGHLGSNDLG
                     RAFEMVTSAPARLLGIDREHGIRPGARADLLIAPAEDAADLVASGPLSRTVLVNGKVV
                     AGTL"
     gene            11019..11972
                     /locus_tag="VIG70_11300"
     CDS             11019..11972
                     /locus_tag="VIG70_11300"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8251187.1"
                     /translation="MLTGLIALVLSAAAISQESYPSRAIRLVVPYAPGGVSDITGRIV
                     AQKMTELLGQPMVVENRAGAGGMVGTGAVAKSPPDGYTIVLSSLSAYAIGPRLVKAPL
                     YDPIADFTPIAAVALSPTILTINTALPFQSLKELIAFGKANPGKLTYGSSGIGSVAHI
                     SAEMLRASTGIELVHVPYKSAAQAYPDMIAGSVSMVFDALPSAIQHIKAGKARPLAMM
                     SDRRASLVPDVPTFAEAGYPEATLRLWVGLHGPAGLPAPIVQKLNDAAAKAVASPDVR
                     ERFTAVGADAYSVSPREFAELVRADVERLGKMMAAAGIKGE"
     gene            11976..12983
                     /locus_tag="VIG70_11305"
     CDS             11976..12983
                     /locus_tag="VIG70_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251188.1"
                     /translation="MTRILFAFIALAFTGAALAQKATPVRFALDWRFEGPAAPYLVAI
                     DKGYYKAEGLDVSIDPGTGSVEGINRVASGAYDVGFADINILVKYRDNPRNLPVKMVM
                     MVYDTPAFSIVTLKKNGIAKPKDLEGKVLGAPAADGAWAQWPIFVQANKIDAAKVKVD
                     NIGFPVREPMLAQGKVDAITGFWFSSFMNLKAQGIPPNEIVVLHMRDWGLDLYGNGII
                     VNPDFMRFSPKAVAGFVKGTIRGIQETLRDPEAAVDAVIKRNPIAKRDVELERLKLSL
                     ERNFVTADVRKNGIGAVDMKRLERSIEQIGLTFQYSNKPKAADIFTAQYLPPREQRLV
                     K"
     gene            13009..13758
                     /locus_tag="VIG70_11310"
     CDS             13009..13758
                     /locus_tag="VIG70_11310"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251189.1"
                     /translation="MAYGRGATAVRAIDEVSLDIARGEFIAVVGPSGCGKSSLMRLIS
                     GLHPPLGGSLTIEGKPVAGPLKSVGMAFQNSNLLPWRNAIDNVLLPLEIVEPYRSDFS
                     RQRYEEKARNLLHSVGLAGFTDKHPWELSGGMQQRASICRALIHEPEILLLDEPFGAL
                     DAFTREELWCALRDLQAARKVTVMLVTHDLREAVFLADTVYVMSNRPGRIVRRCAVPI
                     ARPRDLEVTYTQPFQDIVHELRGLIGKKAGG"
     gene            13751..14524
                     /locus_tag="VIG70_11315"
     CDS             13751..14524
                     /locus_tag="VIG70_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8251190.1"
                     /translation="MDRRDFAIKVLSPCAFVLALFLSWEAVCRIFNVPLAILPAPSDV
                     FAALWQYRKPIADNSWVTLWTTLAGFGIATAFGLALGIAVGWHRAIYSGIYPVLIGFN
                     SVPKVAVVPVLILWFGLGEIPAIMTAFLISFFPIVVNVATGLATTEPELEDVLRALGA
                     SKLDIMRKVGIPRSLPYFFGSLKVAITLAFVGAVVSETVGSNKGIGKLMLDAQAAFQV
                     PIVFAGLIVLAVLGILLYAATAAIEARFTGWAFRGQANR"
     gene            14533..15048
                     /gene="gpt"
                     /locus_tag="VIG70_11320"
     CDS             14533..15048
                     /gene="gpt"
                     /locus_tag="VIG70_11320"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: protein motif:HMM:NF006613.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEY8251191.1"
                     /translation="MARIPYEDEIIISWVELHRDARYLSEVLHAKGPWKGIIAVTRGG
                     LVPAALVARELDIRLVDTICVTSYDAGSAGSAAQQQGKVQVLKGVSGQGEGYLLIDDL
                     VDTGKTAQTVRQLLPKAHFACLYAKPAGRPIVDTFVKEFKQTKWIHFPWDIEYKFATP
                     IKDGGRVVAGP"
     gene            complement(15264..15428)
                     /locus_tag="VIG70_11325"
     CDS             complement(15264..15428)
                     /locus_tag="VIG70_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251192.1"
                     /translation="MKTLFATVFAAMLALMALGVHAAPLTSSTAWFADAHSPLPADDD
                     KDKEKDKKDG"
     gene            15570..16289
                     /locus_tag="VIG70_11330"
     CDS             15570..16289
                     /locus_tag="VIG70_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8251193.1"
                     /translation="MERLDHVLVVDDDKEIRKLLGEYLERNGFRVSLATDGKEMRRTL
                     EQSRPDIVVLDLMLPGDSGLALCRDLRAESSLPVIMLTARAEEVDRIVGLEMGADDYL
                     AKPFSPRELLARIKSILRRTRGLAPVRGDVHRVRFAGWTLDPAARQLVAPDGVIVALS
                     GAEFRLLTVFVEHPNRVLDRNQLMDLTVGRDGTPFDRSIDVQVSRLRVRLRDDAREPR
                     IIKTVRSEGYVLAAAVERIDR"
     gene            16286..17662
                     /locus_tag="VIG70_11335"
     CDS             16286..17662
                     /locus_tag="VIG70_11335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8251194.1"
                     /translation="MKLLPASLFGRTLLVLAAGLVLAQLGSMAIHLFDRGSSVYRLAS
                     LQMAARIGQAARILNRLPAGERHKVVEEVSGPHLRVALSDRPVAVPSGFTEHDPYESE
                     FTASVQRRIGAPWPIRVDINPHPRSSSAARQDFQLWMARHFYFLLPEPFSIVVQVALE
                     DGTLAVFDVNVPQEPLSRFESLAPLLLLLLLVCFVLAALLVRMTNRSLLWLARAADAI
                     GQDPPGEPLAESGPSEIRRVIAAFNRMQERVRRYLVERGRLLSAISHDLKTPITRLRL
                     RAEMLADLELRAKMLRDLDEMQTMVGTTLDFFAAVGKDAERQPVDIGALIESVCEDRR
                     EAGQALSVQGAPIGPYRADPQALRRCLENLIENAVRYGDSADIEIRDSPQRLRIAIRD
                     RGPGIPEPELERVFEPFYRLDTSRNMDSGGTGLGLSIARNIARWHGGEVTLSNAAPGL
                     VAELALPR"
     gene            17678..18346
                     /locus_tag="VIG70_11340"
     CDS             17678..18346
                     /locus_tag="VIG70_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY8251195.1"
                     /translation="MQLVIGNKNYSSWSMRPWVLLKQAQIPFEEVQLKFDEAGGGLRV
                     AGIEKYSAARKVPVLLIDGEPVWDTLAICETAAELFPEKNLWPEDELARRVARSACAE
                     MHSGFQALRGKMPMNIRSSHPGKGLTPESRKDIDRVVALWTGCRERFGGAGNFLFGRF
                     SIPDAFYAPVVMRFETYDVKLPPLAQAYCESVRALAAVREWVEGARRETEFVAEDEPY
                     AKGK"
     gene            18351..19388
                     /locus_tag="VIG70_11345"
     CDS             18351..19388
                     /locus_tag="VIG70_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HEY8251196.1"
                     /translation="MKAYLVGGAVRDELLGLPVKERDYVVVGSTPDEMVRLGFKPVGK
                     DFPVFLHPSTHEEHALARTERKSGRGYKGFTVYASPEVTLEEDLKRRDLTINAMAKAD
                     DGTLIDPFGGKKDLEAGILRHVAEAFAEDPVRILRVARFAARFGFSVADETMQLMHEM
                     VDSGEADYLVAERVWQEFARGLAEPHPELMFEVLQRSGFLEKAFPELRSWPKRFSGSI
                     PARFALLTWPLNESEIKTLCKRLRAPNEVQELALTGCRSKAGLKASTPEALLGLFKTA
                     DALRRPERFAELLEAARIAEPGIDTARIERARGAAAAVDAGAIAAKAASPSQIAKRVD
                     EARLKAIKELC"
     gene            complement(19372..19557)
                     /locus_tag="VIG70_11350"
     CDS             complement(19372..19557)
                     /locus_tag="VIG70_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HEY8251197.1"
                     /translation="MVKWLVTVLLILVVFSVVAQRFGAWRLPGDFSVPVRGRIYYIPL
                     ATTLVFSLLVWLVSKVL"
     gene            19556..20179
                     /locus_tag="VIG70_11355"
     CDS             19556..20179
                     /locus_tag="VIG70_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8251198.1"
                     /translation="MIELYTWSTPNGRKVSIMLEECGLPYNVHKINIGTNTEQFTPQY
                     LKINPNGKIPSIVDPEGPDGKPIAMMESGAILLYLAGKTGKFLPRSVRGKYEALQWLM
                     FQMGGVGPMFGQAHHFMRAKKDEIPYGSERYGNEAKRLYGVMDQHLAGSKFFAAEYSV
                     ADMVIYPWVARYEWHRVDLAGFPNVKRWYDAVGARPAVAKGMAVPQV"
     gene            20176..21387
                     /locus_tag="VIG70_11360"
     CDS             20176..21387
                     /locus_tag="VIG70_11360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8251199.1"
                     /translation="MRFFLLLIFFVPAVAAGMEQVSIPSLDGKLQLPGYLFDALTPGP
                     RPAVISLHGCGGLLDAKGGLSRNRYRVAEYLNVEGMHMLAVDSFTPRSLKSICETPPR
                     QRTFNYEQRREDVFAAIRWLAQRPNVDRTRIAIVGYSNGGSTVLSALDRTEKLVQGQP
                     IQPRAAVAYYPSCRDFAEMWSYEIAAPLLLMIGALDDWTSPHYCERLHDKVKRAQQGA
                     SFEVILYPGSHHGFDGYGPIQMRGGLPTKSGAATVGANPEAREKSLRRMFDFLSEKLE
                     VPLALAHDERLNGHRYAVPPASGFARIDDVAAVPLSEKGRVRYQQWLALGAPKAFAVT
                     EKGGWNFRVEDAEAMQVVLQRCAQPANRCWLYAVDDRVVWSRDVAMRTGPGGLRRSAP
                     PTKAAARLAAN"
     gene            complement(21397..>22767)
                     /locus_tag="VIG70_11365"
     CDS             complement(21397..>22767)
                     /locus_tag="VIG70_11365"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY8251200.1"
                     /translation="LIGLAMLAVMMGAIFIGFPVAFTLLVLAFGFGALVLGGRVFDLA
                     FFQTTGLMRDELLAAVPLFVFMGYVVERAGLMERLFHALRLALAPLRGSLFAVVIVTA
                     TVFAMATGIVGAAVPLLGLMAAPVMLRAGYDSRLAAGAITAGGTLGILIPPSVMLLVL
                     GPVIGVSVVDLYAAAVVPGFMLAALYLAYVLIRCQIEPRLGPPLPKALRAASILAVAR
                     EAAIGVVPLVVLIAATLGSILGGLATPTEAAGVGALGALGLALAYDRMDSAGLKQVLL
                     QTLATSSMVLFLAVASNVFGAVFARLGTAAWITEWLLGLNIPPTLMLLLIVALVFLLG
                     WPFEWPAIVLVFVPIFYPVVVALGIDLVWFGALVAVTLQTAFLSPPVAMSGYYLKQVV
                     PDWPLATIFKGMLEFMAIQVFAIGLLLAFPAIATWLPERLNRPPPALDIYDIEPYPDE
                     RGSYQT"
BASE COUNT         3802 a         7637 c         7696 g         3632 t
ORIGIN      
        1 gtgaaaaacg gaaactggtc ggcccgcggc atggcgtagt ccatgaagct cgcggtctgg
       61 agctgtccgt cggcgtcgta gacgatgtgc tccatcagtg cctggccgac gcccgccacg
      121 atgccgccgt gcgtctggcc atggatgatc agcgggttca ccgcgcggcc gacgtcgtcc
      181 accgaggtat agcgcaccac ttcgacgacc ccggtttccg ggtcgatctc cacctcgcac
      241 accgcgcagc cgaaggcata ggagggcagg gcgtcggtgt tgtcgtgctc gccggcgagc
      301 ggcgccaggt cgaacaggcc gatgcggcgg tcggtgcctt tcacccggaa ttcgccgttg
      361 gcgaactcga tatccgccac cgcggcttcg agcttctcgg aggccgcttg tctgccctgc
      421 tcgacgatgc gcttgactgc aacgtcgatc accacggcgc ccatgcgcat cgagcggccc
      481 gaatgcgtgc cgccgccgat ggatgagacg tccgtgtcgc cggcgtgcaa gcgcacctgc
      541 gccatatcca cgccgagcca gtcggacacg agctgcgcga agctggtctc gtggccctgg
      601 ccggaggaga tggtgccgag cacgacgtcc acggtgccat ccctcttcac cgtgatctgc
      661 gcgcgctcgc gcggtgcgcc ggtgttgagc tcgatgtagt ggcccatgcc gatgccgcgg
      721 agctttcccc ttgaacggga tgcgcttcgt cgtttctcaa acgaagacca ttccgccagt
      781 tctaaagcac gcctctgcac tgccggatag tcgccgctgt cgtacacgag cccctggttg
      841 ttccggtagg gaaacgaccg gatcagattg cgcgccctga gctcggcgcg gtcgaaacca
      901 tgcttgcgtg ccgccaggtc gatcagccgc tcgatcacga acatcacctc cggccggccg
      961 gcggcgcggt agggcgacgt cgccatggtg ttgctggtga ccgcgcgcgc ccggaaggcc
     1021 gcgcagggca cgtgatagac cgaggtcgcg acgccgacgc ccttggtgag cggaatatag
     1081 gaaaacggat acgcgccgac gtcgctggtg ttcgcggagc ggaacgcgag gaagttgccg
     1141 tccttgtcca gcgcgagctc ggcctgcacc gagagggcgc gcgcgtggtc gtcgctcaag
     1201 aagcattcct gccgctcggc gcgccacttc accggcttgc cgaggcgctt cgccgcccag
     1261 gccacgagcg ggatctccgg gcacagatag ttgcgcgtgc cgtagttgcc gccgacttcg
     1321 cgcgccacga agcgcaccgc gctctccggc acgccgagcg ccgccgccgc atcggcgcga
     1381 aagcgctgcg cgctgccggt gggagcgtgg atcgtatagc gcccgtcggc gaacgcggcg
     1441 accacggcgc gcggctcgag cggcacgccg gtgatgcggt tgatggcggt gtcgaggcgc
     1501 acgacgtgcg cggccgactg gaatgctttt tcaacatcgt tcccaacatc ggcgtcgacg
     1561 agggtggttg cgtggggagg aaggacctcg tactccacct ccaccgcgtc ggcgccgtcc
     1621 tttgccgcca tcggcgttcc ggcgatcacc atggccaccg cctggccgac gaagcgcact
     1681 tcgtcgacgg gcagcgccgg gtagggcggg atatggaacg gcgagccgtc ccggttctgc
     1741 agcttcactt cgtgcgggtt cacggtgacc ggccgcgtcg ggatcggctg cagcccgtcg
     1801 gcggcggcgt ccttcccggt caggaccgcg atgactccgg aaatcccggc ttttatcgaa
     1861 agaatcttcg cgtgcgcatg cggcgagcgc accatgcacg cgtagacctg gccggggaga
     1921 ttgacgtcgt cggcgtaaca gcccgcgccg cgcagcaggc gctcgtcctc cttgcgcagc
     1981 accggccggc cgatcgttgt ggattgcgca accgacatgc ggctcgtagt atttcacggc
     2041 cacaaggagg atttcatgca acgcatcgtg ctcgggctcg tgctcgccgc atctgccgcc
     2101 ggcgccgtag cccagaccaa gcttcccttc cgcctcaact ggaccgcctc gggcgagcat
     2161 gccgcctact tcgtcgcccg agacaagggc ttctatggcg acgaaggcct cgaagtcgag
     2221 atcctcgacg gcagcggctc caccaccgtc ctgcagctcg cggcgaacgc gagcaacccg
     2281 gtggtgcagg ccgacgcttc caccatgatg cgcggcatcg tgaacggcat gccggtgaag
     2341 gcggtcgctg tgccgctgca gatcagcccc tccgccttca tctaccgcgc cgacgcgccg
     2401 cgcccgacca aggtgtcgga ggtgaagggc atgcgcatcg cgatgaccgc gggcgactcg
     2461 agcatgaccg tgttcactgc cttcctcggc aagatcggcc tcaagctcga ggacgtgcag
     2521 atcatcaccg tcgcctcgac cgctgccaag gaccaggcga tgctcaacaa gcaggccgac
     2581 gcgctcatcg gcttcttcat ggaccagggc gtgcgcctcg agccgcagac cggggtgaag
     2641 atcggctaca cccgcctcta cgatctgggc caggtcactg cactctcgag cgccatcgtc
     2701 gccaacagcg agtggctgaa ggacgccaag aaccaggaca cgctgcgccg cttcctgcgc
     2761 gcttcgcagc gcggctggca atacgccttc gacaaccgcc gcgaggccgc cgagatcttc
     2821 attaaagcga agcccgcgca acaactgaac atcgcgcagg gcacgctcga gggcgcgatg
     2881 acgctggtgc gcaccgagcg cagcaaggga atgccgatgc tcgcttccgc gccccaggac
     2941 tggaaggaca cgcaggagct gctcgctacg tacgccaagg tgaagccgat gcccgatctc
     3001 gacgcgtact acacgaacgc gtacctctcc gaggcgcctt acctgccgaa gagatgatcc
     3061 tcgagcagct gaagaaggcc gggatcgacc tggttgcgag ccttcccgac cagtggctcg
     3121 gcgatctcat caagcagtgc gaggcggatc ccgcgatcac gcacatccgg ctcgcgcgcg
     3181 aggacgacgg cgtcggcatc tgcgccggcg cgtggctcgg cgggcgcaag gcggcgctcg
     3241 tgtgccagag ctcgggcgtg ctgctggcgg tgaacgcgct cggcggcatc gccttccacc
     3301 accagatccc gctgctggtg atcgcggcgc accgcggcca ctacgacgac aaccagtact
     3361 accagatgta caaggggcgc atctgcgagc cggtgctgaa ggcgatgggc ctgccgcact
     3421 gggtgatcga cggccccgag gggctgccga tgatcggcga cgccgcgcgc caggcgtatc
     3481 tcaaccggct gccggtcgtg gtgctgctgc gaaagaaggc gctgcaatga agcgtctcgc
     3541 actcgcgaaa atggtgaagc agcggctgcg caaggaggac ctcgtggtct gcggcctggg
     3601 cgccaccgaa gcggcgttca aggaagccgc gccagccaat ccgacttact acgcctccga
     3661 cccgatgggc atctgggcct cgctcgcgct tggcctcgcg ctcgcgcgcc cgaagaatcg
     3721 cgtgacgctt ctcgccggcg acggcgatct cctcatgaac ctgcaggtgc ttgccaccat
     3781 cgccacggcg ccggtgccga attaccgcat cgtcgtgttt cataacggcg cgtattcctc
     3841 caccggcggc cagccgctcg cgggcaagct ttcattttct ggtgttgcga agagtgccgg
     3901 tttcgaatgg gcgcatgaag cgcgcaccga ggacgagacc gcgaagtgcc tcgacgagct
     3961 ctacgcgcgg ccggggccgg cgctgctcga cgtgtggctg gaggacgagc cctgtccgac
     4021 cgcgccgccg gggccgctgt cgcaggtcga ggtcagaacg ttgttcatgc gagatatttc
     4081 ctgaggtcga ccggcgggta gacgactttg aggtagcgct tgcgcttgtc ggccggcagc
     4141 gtggcatcga tgatcacctt ggctacgatg ccgtgctcgt ccgagctcgg gtcgagctcg
     4201 ttggtgtgcg cgccgtgcac cgcggagaag ccgcggtccc agcgcgcgcg cgtcgagagc
     4261 gcccacatca cgtcggcagg gtcgaagatg tcgacgtcgg cgtcgaccac gatcaccgtc
     4321 ttgaagttga cgtgcgccgc catcgcggcg agcgccacgt tcttcgcctc gcccggctcc
     4381 ggactgtcca gcgcgatcag cgccgtgaag ccgccggagt agggcggcag gtgcaccgcc
     4441 tttacgcgcg ggctgatgtg cctgacaaag cgctcgagaa gcggctcgcg cgtgatcatg
     4501 ttgcctacgc tcacatgctc gtagccgccg ccgttcatcg tctggaagat cgggttcttc
     4561 cggtgcgtga tgcgtgtgat gcgcgcgacg tgcttcgggt cggcaccgga atagtgcccg
     4621 gtgaactccg ccatcggccc ttccatctgc cgctcgccgg cgaggacttc tccctcgaga
     4681 ataatttccg cagaagcagg cacaaggacg tcgcacgtca gcgctcgcac caccggaatc
     4741 ggcgcctgcc tgagagcgcc tgcgacttcg tactcgtcgc cggcgtagcg aaacgccgcc
     4801 gccatcatca ccaccgggtc ggggccgagc gcgacgcaga acggcaggtt cttcccctgc
     4861 ggctcgatct tgtcgaagaa cgccttgatg tgacgccaga tgttcatgct gatgccggcg
     4921 cgatcggcgc cgtagatctg catgcggttg taggagaggt tgtggcgacc ggtctcgggg
     4981 tcgcgcgcga tcaccacgcc cgcggtgatg aacgggccgg cgtcgagcgg cgagtgcgtc
     5041 gggatcggca atttcgaaag atcgggtttt tccaccacga tctcctggca ctggggcttt
     5101 cccttctgaa tttcgaagcc gaggggcttc tccgtggcag ccgccatgcg agcgcgcacc
     5161 tcgggctcgg tgcatcccag cgcgagcgcg atgcggctgg gcgagctcaa cagcgctccc
     5221 gtgaccggga tgtcgtggtt gccgaccttg tgaaaatacg cggccttgcc cgcgcgctcg
     5281 catgcgttca gtacggcgcc gagctctttc accgggtcga ccgcgcgccg gatctcgtgg
     5341 atttctccgg accctcttac tctttggaga aactcccgca attccatcag taggcccaac
     5401 cggacgcagg taactgcgca gcctcgagcg ccgcgccgga gaggccgccg ggcaggtcgt
     5461 cgagcccggc gaccttgccc gggttcatca ggagatgcgg atcgaccgcg cgcttgaaat
     5521 cgatctgcgc cgcgtcgaga tctttcatgc cgccattctg caggaggaag gtgtgcgcgt
     5581 tggcgatgcg cacgccctcg gcctcgaacg ccttgtagag gcgcgtcatc tgctcgaagc
     5641 cctggtagcg gaagagcggc acgccctccg ccgtcatgta acccgcgaag cgcttgaact
     5701 cgaggtgcag catcacttcg gcgccgagct tgcggtgcac gcggccgatc gcgccaagcg
     5761 tgccatcctt ggggaagatg cataccaggt aggtaagtga gcggtccgca cgcagcgcgt
     5821 gcatcgtggt gtggccccag gtgtactcgt agatgttggc ctgcggcacg cacatcgtca
     5881 cgcttccgcc gaagtctcgg acgagcgctt cgaacgcctc gcgcgtctcg gcgccgatca
     5941 tgagcagggc cgcagccctg ccttccggca agtggggttt aagaggaaca aaaaagccgg
     6001 ggatcggcca ggcgtgcacg ctgaccagct tcttcagcac gccgtcggcg gtggctagcg
     6061 ccatgccgaa cgcggcggct cgctcgaagt cgtcgaacgc cacgatggtg tcgagccatt
     6121 cgtaagccgg cgcgagcggc aactcgacct cggtgacgag gccgttcacg ccgtaggcgt
     6181 ggtgcacgag gttgacgtcg tcgccgcgca actccaggac ccgcggcttc tcctcgatgg
     6241 tgatgatctt caggccgaga aggtttccgc ggtcgcgcag gatgccccag ttgacggtgc
     6301 cgacgccggc gtggccgccg ccgacgaagc cgccgagcgt ggctagcttc ttggtagacg
     6361 ggtgcatgcg cagctcgcgc ccgccttttc ggagctgctc gtcgagcgcg cccaggcgga
     6421 tgccggcctg cgcccgggca acgtttccct tctgaaagac caagcggtcg aggccgacca
     6481 tctcgaccac cgctccgccg gcgagcggca ctgcctggcc aaagttcccc gtaccgccgc
     6541 cgcgcgcgac cagcggcacc cggtggcgcg cacacgcgga ggcgattgtc acgacctctt
     6601 gttcatccct tggacttacc accacttccg cttgccggcc ggcgagcgcg gccgacatga
     6661 tcgggctgaa ccagctgaag tcggcgctct tcacgcgcag ggctgcagcg tcccgcgaat
     6721 ggggaatatt gcccagagct tcgaggaaat cagccttcac ggcgtaccgc gctttcgtgc
     6781 catcggcgca gcgcgaaatg gttgaccgcg gtcaggatca cgaagatcag gatacccgtg
     6841 ccggtgatca tgaagagcgc ggcgaacatc cgcggtattt gcagctggta gccggcctcc
     6901 aggatccggt aggcgagtcc ggactggatg ccgcccgagc cggcgacgaa ctcggcgacc
     6961 accgcgccga tcagcgcgag gccgccgctg atacgcagtc ccccgaggaa gtagggcagc
     7021 gcgctcggca ggcgcaggta ccagaacacc tggcggcgcg aggcgccgta gagctgcagc
     7081 aggttcaaga ggttgtggtc ggtgctgttc aggccgagcg tggtgttcga cacgatcggg
     7141 aagaaagcca cgatccacgc gcagatgaga agcgccgtgg ccgtgtcctt cacccagatg
     7201 atgatgagcg gcgcgatcgc cacgaccggc gtcacctgca ggatcactgc gtaggggaag
     7261 aagctcatct cgatccattt cgattgcgca aacagcaccg cgatcagcac cccgaggatc
     7321 gtcgccgcgg tgagggcgct cagggtgatc agcacggtga cgatgagcga cggatagagc
     7381 gtcgcccagt cgcgcaccag cgcctgcacg atgacgatcg ggccaggcac gatgtacacc
     7441 ggcactttgt aggcgcgcac cgcgatctcc cacgcggcga ggccgatgac gaggatcaag
     7501 accgggggga tgatcctggt gagggcggct ttcaagcttg catcgcctcg gcgaggcgcg
     7561 ccgacacggt gcggcactgg tcggcatagg cggccgaggt gcggaacgac gcatcgcgtt
     7621 tggtttccac cagtacttca gaccggacgc gacctgggcg ggagctcatc accagaatgc
     7681 gcgtcgagag gtacaccgac tcgaacacgc tgtgcgtgac gaaaacgacc gtccagcgct
     7741 ggctcgccca cagctcgagc aggtcgtcgt tcaggcgaaa gcgcgtgatc tcgtcgagcg
     7801 cggcgaaggg ctcgtccatc agcaggatct tgggctgcgc gacgagcgcg cgcgcgatcg
     7861 acacccgcat gcgcatgccg cccgagagct cgcgcggata ggcgcttccg aagccggcga
     7921 ggccgacctg ctccatggcg cgttccaccc ttggggttgc atcagtcttc tttaccccac
     7981 gaacccgcag cggcaggtag atgttgttga acaccgtcgc ccacggcatg agcgtcgggt
     8041 cctggaatac aaagccgatc tcgtgctgca cgctggacca ttcgagcagg ccgccgctca
     8101 gctcgcccag gcccgcgatc aggcgcagca ccgtgctctt gccgcagccg gagggcccga
     8161 gcaggctgac gaactcgcct tcccggatcg tgagatcgag gccctggagg gcgacgatcc
     8221 cgttggaata gaccttgtcg acgccgcgca gcgcgagcag cggcgcgtcg gcgcgcatca
     8281 attcttgggc ttgaggttca tgccgtgggc cttgttcacg aattgcagcg tgtaggcctt
     8341 cttgacgtcg atgtcggcag gatagacgcc cgccttcacc atgctgtcgg cgaactcgcg
     8401 ccagcgcgcg tcggtcatcg cgccgatgcc gagcttgagc gcgtcgcccg agtcgacgat
     8461 gccgtactcg agcatcttcg cgtggctgta ggcgagctgg tcgtcgccca tctccgggtt
     8521 gtccttcttg atgagcgcgt tcgccgcggt gcggtcgccg tacatgtagc tgtaccagcc
     8581 ctcgatcgtc gcgttgacga agcgctgcac cacgtcgggc ctttccttca ccagctggct
     8641 gcgggtctcg agcgtcgtgg cgtaggagac gtagccgttg tcggcgagga ggtggatcac
     8701 tggcttgacg ccggcctgct gcgccttcag cggctcgctg gtgaggaagc cctgctggat
     8761 cgtcgacttg tcagcgagga agggcgccat gttgaacgtg tagggccgga tctggtcgtc
     8821 ggtgtagccg tacttcgcgc gcaggaactg ccagtagttg ttgcgcgagg ccgacgcgat
     8881 caggatcggc ttgcctttca tcgcggcgag cgagtcgttg ccctggcccg ggtgagcgat
     8941 cagcacgcgc gggtctttct ggaagagcga cgccacgacg accagcggga tctcgttctt
     9001 gacgaagttg aggccgttga agccatccga gcccatgctg aagtcgattg cgctcgcgac
     9061 gagcagctgc gggttgttga tctgcggccc gcccggcctg acggtgacgt cgaggccgtg
     9121 ctttgcgtag atgccggtgg ctacggcctg gtagtagccg ccgtgctcgg cctgcgcgcg
     9181 ccagttggac tggaacgtga gcttcacggg ctgcgcataa gttgcttctg cacagaaaac
     9241 gagcagaact gcagcgatgc gaagaactga tgtcatgacg cctcctcctc gttcggacaa
     9301 caagagtaaa tcaggcttgg tgcagcaagg caagaaaggc gatgccgatg gcgctataga
     9361 acatgaacgc cgactcgcgc ccgctccact gtttcgactg ccagcttgcg aaccactggc
     9421 cgccgatcac catgaagccg ccgaaccaga gcgcgaagcc caggcccagg cccgcgaagg
     9481 cgacgccctt ggcggcatgg aacgcgtccg ccccggaacc gtgcacgcgc agcaggatcg
     9541 ccgcaccaat caggcacagg gccgcggtgg cgaattcggc ggcgacgatc agccagaagg
     9601 cgatgcggtg cgcggcgggc gaggtgatgg cgcgttcccg gagccgcgtg tcggggaaca
     9661 cgctgtccat gccgagcacg tgctcgacca ggcgcaggtt gctttgcggg tcgcgcaggt
     9721 tgttgtacgc gaccagcagc gcgaacaggc cgatcagcgc aacgaacagg gcctggcaga
     9781 gctggatgat attcatagtg ttccggccac cacctttcca ttcaccagga cagtgcgcga
     9841 aagcggcccg ctcgccacga gatcggccgc gtcctcggcc ggcgcgatga gcaggtcggc
     9901 gcgcgcgccc ggccggatgc cgtgctcccg gtcgatcccg agcaggcgcg ccggcgcgct
     9961 cgtcaccatc tcgaacgccc ggccgaggtc gttcgagccc aggtgccctg cgagcagggt
    10021 ccagcgcgcg atctcgagga ggtcgccgct gcccgaaggg acgaaggggt cctggatgtt
    10081 gtcggaggcg gccgccaccg gcacgccggc ggcctgcagc tccagcaccc gggtcaggcc
    10141 gcgcggcgtg agcttggtcg ccgcgcgccc ctgcaggaag aggttcgccg ccggcagcgt
    10201 caccaccccg atgccggccg aagcgaagcc gtcgatcact ttcatcgccg cgcccgcctc
    10261 gagcgcgctc agcgccgagc agtggctcgc gaccaccttg cccgccatgc cgtgcgcgcg
    10321 cgtgcgctcg atcacggcct ggaactggat gcgcgccgcg tcgaggtgct cgtcgagatg
    10381 caggtcgatc ggcttgccgt gccgctcggc ggcatcgaac agcatatcga ggaatttcag
    10441 cggctcgtcg gcgtaggtcg gcgtgccgcc gaccacgtcg gcgcccaggc gcatcgcctc
    10501 gtcgagccat tcgcgcgccg gcacgcccgc cttggtcgcg cccgcggtca tgaaggcgac
    10561 gacctgcagc cgtagtcgct cgcgccagcg ctcgcgcacg ccgaccaagg cctcgacgcc
    10621 gcgcgtgcgc gcctgcgggt cgacgttggc gtgcgagcgg atcgccaccg tgccgcgctc
    10681 gaggcacgcc gccatggtgc gctcggcgcg cgccgccacg tccgcgccgc tcatgtccac
    10741 ggcatagcgg ctgaataccc cgatcgctcc ctgcagcgtg ccatcggggt tgggcagctt
    10801 gcgcaagcgc gtcttgtcga ggtgctggtg cgcgtcgacg agacccggca cggccaaacg
    10861 gccgttgagc tcgacgactt tcacgccggg gccggcctcg atcccgggcg cgacgcgctc
    10921 gatgcgaccg cttgcgccga tcaggatgtc ggcgggcttg cctcgtagga gcagtgcgga
    10981 cacgcgtgct agagtgaatt tctatgaatt cacttcgcat gctaaccggg ttgatcgcct
    11041 tggtgctgtc ggccgcggcg atttcacagg agtcctatcc gtcccgcgcg attcgcctgg
    11101 tcgtgcccta cgcccccggc ggcgtgtccg acatcaccgg ccgcatcgtg gcgcagaaga
    11161 tgaccgagct gctcggccag ccgatggtgg tcgagaaccg cgccggcgcc ggcggcatgg
    11221 tcgggaccgg cgcggtcgcc aagtcgccgc ccgacggcta caccatcgtg ctctcgagcc
    11281 tgagcgccta tgccatcggc ccccggctgg tgaaggcgcc gctctacgat ccgatcgccg
    11341 acttcacgcc gatcgcggcg gtggcgctct cgccgaccat cctgacgatc aacaccgctt
    11401 tgccctttca aagcctcaaa gaactcattg cgtttggaaa agcgaacccc ggcaagctta
    11461 cgtacggctc ctcgggcatc ggcagcgtgg cgcacatctc ggcggagatg ctgcgcgcct
    11521 ccaccggcat cgaactggtg cacgttccct acaagagtgc agcgcaggcc tatccggaca
    11581 tgatcgcggg cagcgtctcg atggtgttcg acgcgctgcc ctcggcgatc cagcacatca
    11641 aggccggcaa ggcgcgaccg ttggcgatga tgtcagaccg gcgagcctcg ctcgtgcccg
    11701 acgtgccgac cttcgccgag gcgggctacc ccgaggcgac gctgcgcctg tgggtcgggc
    11761 tgcacggccc ggcgggcctg ccggcgccga tcgtgcagaa gctgaacgac gccgcggcga
    11821 aggccgtcgc gtcgcccgac gtgcgcgagc gcttcaccgc cgtcggcgcc gacgcgtact
    11881 cggtctcgcc gcgcgagttc gcagagctgg tgcgcgccga cgtcgagcgc ttgggtaaga
    11941 tgatggctgc cgcgggaatc aagggggaat aaccgatgac cagaatcctt tttgcgttca
    12001 ttgcgcttgc ttttacggga gctgctcttg cccagaaagc gaccccggtg cgcttcgcgc
    12061 tcgactggcg cttcgagggg cctgcggcgc cctacctggt cgcgatcgac aaggggtact
    12121 acaaggcgga agggctcgat gtgagcatcg acccgggcac cggctcggtc gagggcatca
    12181 accgcgtcgc ctcgggcgcc tacgacgtcg gcttcgcgga catcaacatc ctggtcaagt
    12241 accgcgacaa cccgcgcaac ctgccggtga agatggtgat gatggtgtac gacacgccgg
    12301 cgttctcgat cgtcacgctg aagaagaacg gcatcgccaa gcccaaggac ctcgaaggca
    12361 aggtgctcgg cgcgcccgcc gccgacggcg cctgggcgca gtggccgatc ttcgtgcagg
    12421 cgaacaagat cgacgccgcc aaggtgaagg tcgacaacat cggcttcccg gtgcgcgagc
    12481 cgatgctcgc gcagggcaag gtcgacgcga tcaccgggtt ctggttctcc tccttcatga
    12541 acctcaaggc gcagggcatc ccgccgaacg agatcgtggt actgcacatg cgcgactggg
    12601 gactcgacct ctacggcaac ggcatcatcg tcaatcccga cttcatgcgc ttctcgccca
    12661 aggcggtcgc gggcttcgtc aagggcacga tccgcggcat ccaggagacg ctgcgcgatc
    12721 ccgaagcggc ggtcgacgcc gtgatcaagc gcaacccgat cgccaagcgc gacgtcgagc
    12781 tcgagcggct caagctctcg ctcgagcgca acttcgtcac cgccgacgtg cgcaagaacg
    12841 gcatcggcgc ggtcgacatg aagcgcctgg agcgctcgat cgagcagatc ggcctcacgt
    12901 tccagtacag caacaagccg aaggcggccg acatcttcac cgcgcagtac ttgccgccgc
    12961 gggagcagcg cctcgtcaag taacctggta aagctcgagc gcgtctcgat ggcctacggc
    13021 cgcggcgcta ccgcggtccg cgccatcgac gaggtctcgc tcgacatcgc tcgcggcgag
    13081 ttcatcgcag tcgtcggccc ctcgggctgc ggcaagagct cgctgatgcg gctgatctcg
    13141 ggcctgcatc cgccgctcgg cggaagcctg accatcgaag gcaagcccgt tgccggaccg
    13201 ctcaagtcgg tcggcatggc gttccagaac tccaacctgc tgccgtggcg caacgccatc
    13261 gacaacgtgc ttctgccgct ggaaatcgtc gagccctatc gcagcgattt ttccaggcag
    13321 cgatacgaag aaaaagcgag gaacctgctc cactcggtcg gcctcgcggg cttcaccgac
    13381 aagcacccct gggagctctc cggcggcatg cagcagcgcg cctcgatctg ccgcgcgctg
    13441 atccacgagc ccgagatcct gctgctcgac gagccgttcg gggcgctcga cgcgttcacg
    13501 cgcgaggagc tgtggtgcgc gctgcgcgac ctgcaggcgg cgcgcaaggt gaccgtgatg
    13561 ctggtgacgc acgacctgcg cgaggcggta ttcctcgccg acaccgtata cgtgatgtcc
    13621 aaccggccgg ggcgcatcgt gcggcgctgc gcggtgccga tcgcgcggcc gcgcgacctg
    13681 gaagtgacct acacgcagcc gttccaggac atcgtgcacg agctgcgcgg cctgatcggg
    13741 aagaaggccg gtggataggc gcgatttcgc catcaaggtg ctttcgccct gcgcgttcgt
    13801 cctggcgctt ttcctttcct gggaggccgt gtgccggatc ttcaacgtgc cgctcgcgat
    13861 cctgccggcg ccctcggacg tgttcgccgc gctgtggcag taccgcaagc cgatcgccga
    13921 caactcctgg gtgacgctgt ggaccacgct cgccgggttc ggcatcgcca ccgctttcgg
    13981 cctggcgctc ggcatcgcgg tgggctggca ccgcgcgatc tattccggca tctacccggt
    14041 gctgatcggc ttcaactcgg tgcccaaggt cgcggtggtg ccggtgctga tcctctggtt
    14101 cggcctgggc gagatcccgg cgatcatgac cgcgttcctg atttcgttct tcccgatcgt
    14161 ggtgaacgtc gccaccggcc ttgccaccac cgagcccgag ctcgaggacg tgctgcgcgc
    14221 gctcggcgcc tcgaagctcg acatcatgcg caaggtcggc atcccgcgct cgctgccgta
    14281 cttcttcggc tcgctcaagg tggcgatcac gctcgcgttc gtcggcgcgg tagtctccga
    14341 gacggtgggc tcgaacaagg ggatcggcaa gctgatgctc gacgcccagg cggcgttcca
    14401 ggtgccgatc gtgttcgccg ggctgatcgt gctggcggtg ctcggcatcc tgctctacgc
    14461 ggcgaccgcc gcgatcgagg cgcggttcac ggggtgggct ttccggggac aggcaaatcg
    14521 ctagaattcc atatggcgcg catcccttac gaagacgaaa tcatcatctc ctgggtggag
    14581 ctgcaccgcg acgcccgcta tctctccgaa gtcctgcacg cgaaaggccc ctggaaggga
    14641 atcatcgcgg tgacgcgcgg cggcctggtg ccggcggcac tggtggcgcg cgagctcgac
    14701 atccggctcg tcgacaccat ctgcgtcacg agctacgacg ccggctcggc gggaagcgcg
    14761 gcgcagcagc aaggcaaggt gcaggtgctc aagggcgtga gcgggcaggg cgagggctac
    14821 ctgctgatcg acgatctggt cgataccggc aagactgcgc agaccgtgcg gcagctcttg
    14881 cccaaggcgc actttgcgtg cctctacgcc aagccggcgg gcaggccgat cgtcgatacc
    14941 ttcgtcaagg agttcaagca gacgaagtgg atccacttcc cttgggacat cgagtacaag
    15001 ttcgcgacgc cgatcaagga cggcggccgg gtcgtcgccg gaccgtaaaa aaaagccccg
    15061 gctggcgggg ccataagctg ctgctcttgg gaggaacttc agtccatcag gatgtgcgga
    15121 taaatgaacc cgaacgagat catcatcacc gcgaccttca gcacgaacac ggtcttctgg
    15181 cggacggtca tttcattgta tttgcgcgtc atcgtggtgc ctccggatca gtgtgcggcc
    15241 caggagcgga tccagtctct ttcctagccg tccttcttgt ccttctcctt gtccttgtcg
    15301 tcgtccgcgg gcagcggcga gtgggcatcg gcaaaccacg cggtggacga cgtcagcggc
    15361 gcggcatgga cgccgagcgc catcagggcc agcatcgcgg cgaacacggt cgcgaacaga
    15421 gttttcatcg cttctccttt cggtaacggg gggaacgaac cccgattaga gcggtggcgt
    15481 gtaacgccga aattgcaccc gggccggctt gtcacactgt gttacatggc ttgacgcgtc
    15541 gcgccggcgg gtgcagatat agcgccgcga tggaacggct cgaccacgtc ctcgtcgtcg
    15601 acgacgacaa ggagatccgc aagctgctgg gcgagtacct ggagcgcaac ggctttcgcg
    15661 tctcgctcgc caccgacggc aaggaaatgc ggcgcaccct cgagcagagc cgacctgaca
    15721 tcgtggtgct cgatctcatg ctgccgggag acagcggcct cgcgctgtgc cgcgacctgc
    15781 gcgcggagtc gagcctgccc gtgatcatgc tgacggcgcg cgccgaggaa gtcgaccgca
    15841 tcgtcggtct ggagatgggc gcggacgact acctcgccaa gccgttcagc ccgcgcgagc
    15901 tgctcgcgcg catcaagagc atcctgcgcc gcacgcgcgg cctggcgccg gtgcgcggcg
    15961 acgtgcaccg ggtgcgcttc gcgggctgga cgctcgatcc cgcggcacgg cagctggtgg
    16021 cgcccgacgg cgtgatcgtg gcgctgagcg gggcggagtt caggctgctc acggtgttcg
    16081 tggaacatcc gaaccgcgtg ctcgaccgca accagctcat ggaccttacg gtgggccgcg
    16141 acggcacgcc gttcgaccgc tcgatcgacg tgcaggtgag ccgcctgcgc gtgcggctgc
    16201 gcgacgacgc gcgcgagccg cgcatcatca agacggtgcg cagcgaaggc tacgtgctcg
    16261 ccgcggcggt ggagcggatc gaccggtgaa gctgcttccg gcgtcgctct tcgggcgcac
    16321 cctgctcgtg ctggcggcgg gcctggtgct ggcgcagctc ggcagcatgg cgatccacct
    16381 gttcgatcgc ggcagctccg tctaccggct cgcctccctg cagatggccg cgcgcatcgg
    16441 ccaagccgcc cgcatcctca atcgcctgcc cgccggcgag cgccacaagg tggtggagga
    16501 agtgagcggg ccgcacctgc gcgtcgccct gagcgaccgg ccggtcgcgg taccgagcgg
    16561 cttcaccgag cacgatccct acgagtcgga attcaccgcg tcggtgcagc gccggatcgg
    16621 cgcgccctgg ccgatccgcg tcgacatcaa cccgcacccg cgctcgtcaa gcgctgccag
    16681 gcaagacttc cagctctgga tggcgcggca cttttacttc ctgttgcccg agcccttctc
    16741 gatcgtggtg caggtcgctc tcgaggacgg cacgctcgcc gtgttcgacg tcaacgtgcc
    16801 ccaggagccg ttgagccgct tcgaatccct ggcgccgctg ctcctgctcc tgctcctcgt
    16861 gtgcttcgtg ctggcggcgc tgctcgtgcg catgaccaac cgctcgcttc tgtggctggc
    16921 gcgcgcggcc gacgccatcg gccaggaccc gccgggcgag ccgctcgccg agagcgggcc
    16981 gagcgagatc cgccgcgtga tcgcggcctt caaccggatg caggagcggg tgaggcgcta
    17041 cctcgtcgag cgtggccggc tgctgagcgc gatctcccac gacctgaaga cgccgattac
    17101 ccgcctgcgc ctgcgcgcgg agatgctggc cgacctggag ctgcgcgcca agatgctgcg
    17161 cgacctcgac gagatgcaga ccatggtcgg gaccacgctc gacttcttcg ccgccgtcgg
    17221 caaggacgcc gagcgccagc cggtcgacat cggcgctctc atcgagagcg tctgcgagga
    17281 ccggcgcgag gccgggcagg cgctgagcgt gcaaggcgcg ccgatcggcc cctaccgcgc
    17341 cgacccgcag gcgctgcggc gctgcctgga gaacctgatc gagaacgccg tgcgctacgg
    17401 cgactcggcc gacatcgaga tccgggattc gccgcagcgg ctgcggatcg cgatccgcga
    17461 ccgcggcccg ggcatcccgg agcccgagct cgagcgggtg ttcgagccgt tttaccggct
    17521 cgacacctcg cgcaacatgg acagcggcgg caccggcctc ggcctgagca tcgcacgcaa
    17581 tatcgcccgc tggcacggcg gcgaagtcac cttgagcaac gctgcgcccg ggctcgtcgc
    17641 cgaactcgcc ctgccgcgct agactttgct catgaagctg cagctcgtga tcggcaacaa
    17701 gaactattct tcctggtcga tgcggccgtg ggtgctgctc aagcaggcgc agatcccgtt
    17761 cgaggaggtg cagctcaagt tcgacgaggc cggcggcggg ctgcgcgtcg ccgggatcga
    17821 gaagtactcg gcggcgcgca aggtgccggt gctgctgatc gacggcgagc cggtgtggga
    17881 tacgctcgcg atctgcgaga cggcggcgga gctgttcccg gagaagaacc tctggcccga
    17941 ggacgagctc gcccgtcgcg tggcgcgctc ggcgtgcgcg gagatgcact ccggcttcca
    18001 ggccctgcgc ggcaagatgc cgatgaacat ccgctcgagc catccgggca agggcctgac
    18061 gcccgagtcg cgcaaggaca tcgaccgcgt ggtcgcgcta tggaccggct gccgtgagcg
    18121 cttcggcggc gccggcaact tcctgttcgg gcgcttctcg atccccgacg ccttctacgc
    18181 gccggtggtg atgcgcttcg agacctacga cgtgaagctg ccgccgctcg cgcaggccta
    18241 ctgcgagtcc gtgcgcgcgc tcgccgcggt gcgcgagtgg gtcgagggcg cgcgccgcga
    18301 gacggagttc gtggccgaag acgagcccta cgcaaagggt aaatgaggca gtgaaggcct
    18361 atctcgtcgg cggggcggtg cgcgacgagc tcctcggcct gccggtgaag gaacgcgact
    18421 acgtcgtggt cggcagtacc ccggacgaga tggtgcgcct aggcttcaag ccggtgggca
    18481 aggacttccc ggttttcctc cacccgagca cccatgaaga gcacgccttg gcaagaacgg
    18541 aaagaaaaag cggccgcggc tacaaaggct tcaccgtcta cgcctcgccc gaggtgacgc
    18601 tggaagagga cctcaagcgc cgcgacctta cgatcaacgc catggcgaag gccgacgacg
    18661 ggacgctgat cgatcctttt ggcggcaaga aggatctcga ggcgggaatc ctgcgccacg
    18721 tagccgaagc ctttgccgaa gacccggttc gcatcctgcg cgtcgcgcgc ttcgccgcgc
    18781 gcttcggctt cagcgtcgcc gacgaaacca tgcagctcat gcatgagatg gtcgactctg
    18841 gcgaggcgga ctatctcgtc gccgagcgcg tctggcagga attcgctcgc ggcctcgccg
    18901 agccgcaccc ggagctgatg ttcgaggtgc tgcagcgctc ggggtttctg gagaaggcat
    18961 ttccggagct caggtcctgg ccgaagcgct tctccggctc gatcccggcc cgttttgcgc
    19021 tcctcacctg gccgctcaac gaatccgaga tcaagacgct gtgcaagcgg ctgcgtgcgc
    19081 cgaacgaggt gcaggagctc gcgcttaccg gctgccgcag caaagcgggc ttgaaagcct
    19141 cgaccccgga ggccctgctc gggctgttca agaccgcgga cgcgttgcgc cggccggagc
    19201 gcttcgcgga gctgctcgag gcggcgcgga tcgcggaacc gggcatcgac accgcgcgca
    19261 tcgagcgcgc gcggggcgcg gccgcggcgg tggacgcggg tgcgatcgca gcgaaggcgg
    19321 cctccccttc gcagattgca aaacgggtgg acgaggcgcg cctgaaggcg atcaaagaac
    19381 tttgctgacc agccagacca gcagcgaaaa caccagcgtc gtggcgaggg ggatgtagta
    19441 gattcgtccg cgcaccggga ccgagaagtc tcccggcaag cgccaggcgc cgaaacgctg
    19501 cgcgactact gaaaacacaa ccaatatcaa caggaccgtc accagccact tcaccatgat
    19561 cgaactttat acctggagca cccctaatgg ccgcaaggtc tccatcatgc tcgaggaatg
    19621 cggcctgccg tacaacgtgc acaagatcaa catcgggacc aacaccgagc agttcacgcc
    19681 gcagtacctg aagataaacc cgaacggcaa gatcccgtcg atcgtcgatc ccgagggccc
    19741 cgacggcaag ccgatcgcga tgatggagtc gggcgcgatc ctgctctacc tcgccggcaa
    19801 gacgggaaag ttcctgccgc gctcggtgcg cgggaagtac gaagccctgc agtggctcat
    19861 gttccagatg ggcggggtgg ggccgatgtt cggccaggcg caccacttca tgcgcgcgaa
    19921 gaaggacgag atcccctacg gcagcgagcg ctacggcaac gaggcgaagc ggctgtacgg
    19981 cgtcatggac cagcacctcg cgggcagcaa gttcttcgcc gccgaatact ccgtcgccga
    20041 catggtgatc tacccgtggg tcgcccgcta cgagtggcat cgcgtggacc tggcggggtt
    20101 tccgaacgtg aagcgctggt acgacgccgt gggcgcgcgt ccggcggtgg ccaaagggat
    20161 ggcggtgccg caggtgtgag gttctttctt ctcctcatct tcttcgtgcc ggccgtcgcc
    20221 gccggcatgg agcaggtgtc gatcccgtcg ctcgacggga agctgcagct gccgggctac
    20281 ctgttcgatg cgctcacgcc cggcccgcgg ccggcggtga tctcgctgca cggctgcggc
    20341 ggcctgctgg acgcgaaggg cggcctcagc cgcaaccgct accgggtcgc ggagtatctc
    20401 aacgtcgaag gcatgcacat gctcgcggtc gacagcttca cgccgcgaag cttgaagtcg
    20461 atctgcgaaa cgcccccgcg ccagcggacg ttcaactacg agcagcggcg cgaggacgtg
    20521 ttcgccgcga tccgctggct cgcgcagcgg ccgaatgtgg atcgcacccg catcgcgatc
    20581 gtgggctact cgaacggcgg aagcacggtg ctgagcgcgc tcgaccgcac cgaaaagctg
    20641 gttcaggggc agccgatcca gcccagggcc gcggttgcct actaccccag ttgccgggat
    20701 ttcgccgaga tgtggagcta cgagatcgcc gcgccgctgc tcctcatgat cggcgcgctg
    20761 gacgactgga cgtctcccca ttactgcgag cgccttcacg acaaggtgaa gcgcgcgcag
    20821 cagggtgcgt ctttcgaggt gatcctgtat cccgggagcc atcacggctt cgacggctac
    20881 ggtcctatcc agatgcgggg cggcctgccg acaaagagcg gtgccgccac ggtgggcgcg
    20941 aaccccgagg cgcgggaaaa gtcgctgcgc cggatgttcg acttcctttc cgagaagctc
    21001 gaggtgccgc tcgcgctcgc gcacgatgag cgcctgaacg ggcaccgcta tgccgtgccg
    21061 ccggcgagcg gcttcgcccg catcgacgac gtcgccgcgg ttccgctgag cgagaagggc
    21121 cgcgtgcgct accagcagtg gctggcgctc ggcgcgccca aggcgttcgc cgtgacggag
    21181 aagggcggct ggaatttcag ggtggaggac gccgaggcga tgcaggtcgt gctgcagcgc
    21241 tgcgcgcagc cggccaaccg ctgctggctc tacgcggtcg acgaccgcgt cgtctggagc
    21301 cgggacgttg cgatgcgcac cggcccgggc gggctgcggc gcagcgcgcc cccgacaaag
    21361 gctgccgccc gtctcgcggc aaattagcgg ggtgaactag gtttgatatg acccgcgctc
    21421 gtccgggtag ggctcgatgt cgtagatgtc gagcgcgggc ggcggccggt tgagccgctc
    21481 gggtagccag gtcgcgatcg ccgggaaggc gagcagcagg ccgatcgcga acacctggat
    21541 cgccatgaac tccagcatcc ccttgaagat ggtcgcgagc ggccagtccg gaaccacctg
    21601 cttcaggtag tagcccgaca tcgccacggg cggcgacagg aaggcggtct gcagcgtcac
    21661 ggcgaccagc gccccgaacc agaccaggtc gattccgagc gccaccacta ccggatagaa
    21721 gatcggcacg aacaccagca cgatcgccgg ccactcgaac ggccagccga gcaggaacac
    21781 gagagccacg atgaggagca gcatcagggt cggcggaatg ttgaggccga gcagccactc
    21841 ggtgatccag gccgcggtgc cgagccgggc gaacaccgcg ccgaagacgt tcgaggcgac
    21901 cgcgaggaac agcaccatgc tcgaggtcgc cagcgtctgc agcagcacct gcttcaggcc
    21961 cgcgctgtcc atccggtcat aggccagcgc caggccgagg gcgccgagcg cgccgacgcc
    22021 ggccgcttcc gtcggcgtcg caagtccgcc gaggatcgac ccgagcgtcg ccgcgatcag
    22081 cacgaccagc ggcaccacgc cgatcgcggc ctcgcgcgcg accgcgagga tggacgcggc
    22141 acgcagcgcc ttcggcaacg gcggcccgag gcgcggttcg atctggcagc ggatcagcac
    22201 gtaggcgaga tacagtgccg ccagcatgaa cccgggcacg actgccgctg cgtacaggtc
    22261 gacgaccgac acgccgatga ccggcccgag caccagcagc atcacggacg gcgggatcag
    22321 gatgccgagc gtgccgccgg ccgtgatggc tcccgcggcg agccgcgaat cgtagccggc
    22381 gcgcagcatc accggcgccg ccatcagccc gagcagcggc accgccgcgc cgacgatccc
    22441 ggtcgccatg gcgaacaccg tcgcggtgac gatgaccacg gcgaagagcg atccccgcag
    22501 cggcgcgagc gcgagacgca gggcgtggaa caggcgctcc atcaacccgg cccgctcgac
    22561 cacgtagccc atgaaaacga atagcggcac cgccgcgagc agctcgtcgc gcatcaaacc
    22621 agtggtctgg aagaacgcga ggtcgaacac gcggcccccg agcaccagcg cgccgaagcc
    22681 gaacgccagc acgaggagcg tgaaggccac cggaaagccg atgaagatcg cgcccatcat
    22741 caccgccagc atcgcgaggc cgatcag
//
LOCUS       DATBSZ010000174         7585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1120239, whole genome shotgun sequence.
ACCESSION   DATBSZ010000174 DATBSZ010000000
VERSION     DATBSZ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120239"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VIG70_11370"
     CDS             <1..357
                     /locus_tag="VIG70_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY8251201.1"
                     /translation="AATSTGGYTGKMPGRVGDSPIIGAGTYADNRACAVSGTGLGEAF
                     IRAAVGHDVCARMRYAGASLAAAAGAALKNVANLGADGGLIAVDRRGNVAMPFNSEGM
                     YRACIDRRGRRTLAIF"
     gene            354..746
                     /locus_tag="VIG70_11375"
     CDS             354..746
                     /locus_tag="VIG70_11375"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEY8251202.1"
                     /translation="MKVELRYAPLPWIGAIAWHYWFVVCDDTGRHRWEVWQTKNAGGT
                     CIGHVHCDLKDPDDGVGGGPSRRVMEWDGEAARRLCEVLKNANAYPYCEHYRMWPGPN
                     SNTFVAWVLREAGIEHALDPRAIGRTFT"
     gene            complement(739..1356)
                     /locus_tag="VIG70_11380"
     CDS             complement(739..1356)
                     /locus_tag="VIG70_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251203.1"
                     /translation="MKPLLVASILLAAASAQAQQKDMTFFITSAGPGNGADLGGLQGA
                     DRHCQTLAKAAGAGQREWRAYLSTDKVNARDRIGKGPWKNAKGEEIARSVDDLHSNSK
                     INKQTGLDEKGQPVNGRGDSPNRHDILTGSQPDGRAVADKDMACGNWTKSGEGSAIVG
                     HHDRMGLRDDDASKSWNSSHPSRGCSQDALRGSGGDGLFYCFAVK"
     gene            1393..2349
                     /locus_tag="VIG70_11385"
     CDS             1393..2349
                     /locus_tag="VIG70_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8251204.1"
                     /translation="MRTVIAAFLVLLSVTAYGQDSWPSRPVRFILPFPPGGGTDILGR
                     LIAERLSAGLGQPVVTENRGGAGGNVGAEAAARSSPDGYTIVLVAPSLAISPTLYSKI
                     NYDPVKDFAPVSLVATVPNVMITQASAPVQNLQEFIAFAKGKPGALNFGSGGAGTSNH
                     LAGELFNIVTGAKLVHIPYKGVNLAMQDVLAGNVHLVFIGIPAAAPHIKAGKLRALAL
                     VAPQRSSALPEVPTVAEAGLRDFEVTTWYGVLAPAGTPRNVVTRLNAELGKIMHSPEL
                     KEKLAATGTEPLTSTPEEFADYIKREIAKWGEVVRKAGVKAD"
     gene            complement(2414..3487)
                     /locus_tag="VIG70_11390"
     CDS             complement(2414..3487)
                     /locus_tag="VIG70_11390"
                     /inference="COORDINATES: protein motif:HMM:NF042665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HEY8251205.1"
                     /translation="MNRLLILLLVLGFAAHAQEPKQFIFAWPLDETALKPRGATTRGA
                     PVTLDAVPGEAWRRLNEPGLSAQERDRRAILAMAGPYRVSFDFLEVVRYDPALKPDAP
                     YQSWATEYVFVAEDRPDFIALQHILVMRMRLEGGKTSEPMVVRHWRQEWRYEAQSLLA
                     YEGANTWTRRAVPATERRGAWTQSVLQVDDSPRYAARGRWQHAAGVSTWISDETWRPL
                     PRREFSVRKDYDVLVGTNRHTITPTGWVQEENNQKMNLPEKRYLAREYGVARYDRIRD
                     YDFSAGERYYARTEPFWAEVRAAWRELEEARGRFGVRAPVDQAQLFVPFFEYADKLVE
                     GGAFDRDAARAFVRKTLQDSYLK"
     gene            complement(3484..4557)
                     /locus_tag="VIG70_11395"
     CDS             complement(3484..4557)
                     /locus_tag="VIG70_11395"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY8251206.1"
                     /translation="MSAPPFRAEHIGSLLRPKRLLEDRSQEDECIVEAIRWQESLGLK
                     VVTDGEFRRESWRLGFVSKVAGFARADAVGNVDLQRDDVGNVMRIGSAPVAVTRIRRT
                     GPIVADEVAFSLQNTKRTVKATIPAPSYLHYPRGRNCVDRDVYPELEQFFNDVVDVYV
                     QELHALHAAGGRYLQIDEVAQTLLCDQKLRDAVRARGDDAEKLIDLYIDLINRVARER
                     PAGMTVGVHMCRGNAMGKWVAEGGYERIAEKSFSRLEVDAFFLEFDTERAGGFEPLRF
                     VPKGKRVVLGLVSTKTPALEDKNVLKRRIDAAARFVPPENLSISPQCGFASHEKGTAL
                     GFAEQEAKMRLVVDLATEVWGEA"
     gene            complement(4554..>7585)
                     /gene="treY"
                     /locus_tag="VIG70_11400"
     CDS             complement(4554..>7585)
                     /gene="treY"
                     /locus_tag="VIG70_11400"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEY8251207.1"
                     /translation="AILAAESRRARCLVIGEDLGTVTAEMRAALNAAGVLSYRPLLFE
                     NKRSKDYPREALVCVTTHDLPTWRGYWDAHDLQLRERLGLTVDARKELALRETEKRKL
                     KATLEREGLDLSARSAHAFIARTPAKLAMLQPEDVLEMVDQANLPGTVDEHPNWRRKL
                     PLALERWSAEPRVAALAGIMAERSVATRAPLVVPAATYRLQFHKGFKFRDATALVPYL
                     AALGVSHLYASPFLKARPGSMHGYDVIDHGQVNPEIGTEAELHELIQRLRKHGMGMVL
                     DLVPNHMGVLHADNAWWLDVLEKGRASPYARFFDIDWARGKLLLPVLGKHYGEALAEL
                     TVEKKNGKWSVRYFDHRFPLNAKSQRGLKAPPRDTLALHRLLEKQYYRLAYWRVASDE
                     INYRRFFEITDLAGVKVEDRTVFEATHGLVARLVKAGGIDGVRIDHPDGLADPKQYFD
                     RLAELFPRRWVLIEKILADHEKLPENWPVHGETGYRFANLLTGVFIDGAAESRVDRVY
                     RSFTGQRRSFEEIARESRWLIMATTLAAELHMLSNWLARIAAGNRSTRDYTASGLRKA
                     LAEIAAQFPVYRTYVSARGVSDGDRRVIGGAVRAAKRASRIADPSVFDFVHGVLTLDA
                     APPSGRRRSEMLRFSMRFQQFSAPVVAKGVEDTAFYRYNRLIALNEVGGHPSRFGIPL
                     KEFHAAAEERARRWPYTMLGSSTHDTKRSEDVRARLAVLSELASGWRITLRRWSGMNE
                     SNISRSDEYHFYQALIGIWPGKVSGDLAERLKAYMLKAAREAKEHTSWINPDAEYEAA
                     LERLVIRSLENKAFVEDFGNTVKRVVHLGLLVGLSQALVKVASPGVPDYYQGTELWDF
                     SLVDPDNRRPVDYELRKKLLKNSEDLLKNLADGRAKMQVIRKGLEVRKALPTLFHGAA
                     YTPLYADAGREENVIAFALCEGARSVIAVAPRLVSRLVEGGDVAPLGAKAWGESRLPM
                     AGEYLNVLTGERHEARGGHVGLAQILATFPVALLVRP"
BASE COUNT         1143 a         2594 c         2555 g         1293 t
ORIGIN      
        1 gcggcgacct cgaccggcgg ctataccggc aagatgccgg gccgcgtcgg cgactcgccg
       61 atcattggcg ccggcaccta cgccgacaac cgcgcctgcg cggtgtcggg caccgggctc
      121 ggggaagcgt tcattcgcgc cgccgtcggc cacgacgtat gtgcgcgcat gcgctacgcc
      181 ggtgcttcgc tcgccgcggc ggcgggcgct gcgctgaaga acgtcgccaa tctcggcgcc
      241 gatggcggtc tcattgcggt cgaccgccgc ggcaacgtgg cgatgccgtt caacagcgaa
      301 ggcatgtacc gcgcgtgcat cgaccgccgc gggcggcgca cgctggccat cttttgaagg
      361 tcgagcttcg ctatgcgccg ctgccctgga tcggcgcgat cgcctggcat tactggttcg
      421 tggtgtgcga cgacaccggg cgccaccgct gggaggtgtg gcagacgaag aacgccggcg
      481 gcacctgcat cggccatgtg cactgcgacc tcaaggatcc ggacgacggc gtcggcggcg
      541 ggccttcgcg gcgggtcatg gaatgggacg gggaagccgc gcgccggctt tgcgaggttc
      601 tgaagaacgc gaacgcgtat ccctactgcg agcactaccg catgtggccc gggccgaaca
      661 gcaatacctt cgttgcctgg gtgctgcgcg aagccgggat cgagcacgcg ctcgatccgc
      721 gggccatcgg caggacgttt acttgaccgc gaagcagtag aacaggccgt cgccgcccga
      781 gccgcgcagc gcgtcctggc tgcagccgcg cgagggatgc gacgagttcc aggacttcga
      841 ggcgtcgtcg tcgcgcaggc ccatgcgatc gtggtgcccg acgatcgccg agccttcgcc
      901 gctcttggtc cagttgccgc aggccatgtc cttgtcggcg acggcgcggc catcgggctg
      961 cgagccggta aggatgtcgt ggcggttcgg gctgtcgccg cgcccgttga ccggctggcc
     1021 cttctcgtcg agcccggtct gcttgttgat cttgctgttc gagtgcaggt cgtcgacgct
     1081 gcgcgcgatt tcctcgccct tcgcgttctt ccagggacct ttgccgatcc gatcgcgggc
     1141 attgaccttg tcggtgctga gataggcgcg ccactcgcgc tggccggcgc cggcggcctt
     1201 ggcgagggtc tggcaatggc ggtcggcgcc ctgcaggccg ccgaggtccg cgccgttgcc
     1261 cggtcccgcg ctggtgatga aaaaggtcat gtctttttgc tgcgcctggg cgctcgccgc
     1321 agcgagcagg atggatgcta cgaggagggg tttcatgacg ctgcaaacac gtgcggcgat
     1381 aagatattca acatgcgcac cgtaatcgcg gcgttcctcg ttctactttc cgtaactgca
     1441 tacggccagg actcctggcc ctcgcgcccg gtgcgcttca tcctgccgtt cccgcccggc
     1501 ggcggcaccg acatcctcgg ccggctgatc gccgagcggc tgtcggcggg cctcggccag
     1561 ccggtggtga ccgagaaccg cggcggcgcc ggcggcaacg tcggcgccga agccgcggcg
     1621 cggtcgtcgc ctgacggcta caccatcgtg ctcgtcgcgc cgtcgctcgc catcagcccc
     1681 acgctctatt caaagatcaa ctatgacccg gtgaaggatt tcgcgccggt gagcctggtc
     1741 gcgacggtgc cgaacgtgat gatcacgcag gcctcggctc cggtgcagaa cctgcaggag
     1801 ttcatcgcgt tcgcgaaggg caagcccggc gcgctcaact tcggctcggg cggcgccggc
     1861 acctcgaacc acctggcggg cgagctcttc aacatcgtca ccggcgcgaa gctggtccac
     1921 atcccgtaca aaggcgtgaa cctcgcgatg caggacgtgc tcgcgggcaa cgtgcacctc
     1981 gtcttcatcg gcatcccggc ggccgcgccg cacatcaagg cgggcaagct gcgcgcgctc
     2041 gcgctggtcg cgccgcagcg ctcgagcgcc cttcccgagg tgccgacggt cgccgaggcg
     2101 ggcctgcgcg actttgaagt gaccacgtgg tacggcgtgc tcgcgccggc gggaacgccg
     2161 aggaatgtgg ttacccggct caatgcggag ctggggaaga tcatgcactc gccggagctg
     2221 aaggagaagc tcgcggcgac cggcaccgag ccgctcacca gcacgcccga ggagttcgcc
     2281 gactacatca agcgagagat cgcgaaatgg ggcgaggtcg tgcgcaaggc gggcgtgaag
     2341 gcggactaat tcggggacgg agccctaact ccacggctgg cgcagctttc ggagttaggg
     2401 ctccgtcccc gaattatttc aggtagctgt cctgcagcgt tttccggacg aaagcgcgcg
     2461 cggcgtcgcg gtcgaaggcc ccgccttcga cgagcttgtc ggcgtattcg aagaacggca
     2521 cgaaaagctg cgcctggtcg accggcgcgc gcacgccgaa gcgcccgcgc gcctcctcga
     2581 gctcgcgcca cgcggcgcgc acctccgccc agaacggctc ggtgcgggcg tagtacctct
     2641 ctcccgcgct gaagtcgtaa tcccggatgc ggtcgtagcg cgcgacgccg tattcccgag
     2701 ccaggtaccg cttttccgga agattcattt tttgattgtt ttcctcctgc acccagccgg
     2761 tcggcgtgat ggtgtggcgg ttggtgccga cgagcacgtc gtagtccttg cgcacgctga
     2821 attcgcgccg cggcagcggc cgccaggtct cgtcgctgat ccaggtggaa acgccggccg
     2881 cgtgctgcca gcgcccgcgc gctgcatacc gcggtgagtc gtcgacctgg agcacgctct
     2941 gggtccaggc gccgcggcgc tccgttgccg ggaccgcgcg ccgcgtccac gtgttcgcgc
     3001 cttcgtaggc gagcagcgat tgcgcctcgt agcgccattc ctggcgccag tggcgcacca
     3061 ccatcggctc actcgttttg ccgccttcga gccgcatacg catcaccagg atgtgctgca
     3121 gggcgatgaa gtccggccgg tcttcggcga cgaagacgta ctcggtggcc cagctctggt
     3181 agggcgcatc cggcttgagc gccgggtcgt agcgcaccac ctcgaggaag tcgaagctca
     3241 cgcgataagg gcccgccatc gcgaggatcg cccgccggtc gcgctcctgc gcgctcagcc
     3301 ccggctcgtt cagccgccgc cacgcctcgc ccggtaccgc atcgagcgtc accggcgcgc
     3361 cgcgcgtggt cgcaccccgg ggcttgagcg ccgtttcgtc cagtggccac gcgaaaatga
     3421 actgcttcgg ctcttgcgca tgggccgcga accccagcac gagcaacaag atcaaaagcc
     3481 tgttcatgct tcgccccaca cctcggtggc aaggtcgacg acgagccgca tcttcgcctc
     3541 ctgctcggcg aagccgagcg cggtgccctt ctcgtggctg gcgaagccgc actgcgggct
     3601 gatcgacagg ttctcgggcg ggacgaagcg cgccgccgcg tcgatcctcc tcttcaggac
     3661 gttcttgtct tccagcgccg gcgtcttggt ggagaccagc cccagcacca cgcgcttgcc
     3721 cttcggcacg aagcgcaggg gctcgaagcc cccggcgcgc tcggtgtcga actcgagaaa
     3781 gaacgcgtcg acctccagcc gggagaacga tttttccgcg atgcgctcat agccgccctc
     3841 ggcgacccac ttgcccatcg cgttgccccg gcacatgtgc acgcccaccg tcattccggc
     3901 cggccgctcg cgcgccacgc ggttgatgag atcgatgtag agatcgatca gcttctcggc
     3961 atcgtcgccg cgggcgcgca ccgcgtcgcg cagcttctgg tcgcagagaa gggtctgcgc
     4021 cacctcgtcg atctgcaggt agcgcccgcc ggcggcgtgc agcgcatgaa gctcctggac
     4081 gtacacgtcg accacgtcgt tgaagaactg ctccagctcc ggatagacgt cgcggtcgac
     4141 gcagttgcgg ccacgcggat agtggagata ggaaggggcc gggatcgtcg ccttcaccgt
     4201 gcgtttcgta ttttgaagtg aaaaagcgac ctcgtcggcg acgatcggcc cggtgcgccg
     4261 gatgcgggtc acggcaaccg gcgccgagcc gatgcgcatc acgttgccga cgtcgtcgcg
     4321 ctgcaggtcg acgttgccca ccgcgtcggc gcgcgcgaac ccggcgacct tggagacgaa
     4381 gcccaggcgc cacgattcgc gccggaactc gccgtcggtc accaccttga ggccgaggct
     4441 ctcctgccag cgaatcgcct cgacgatgca ttcgtcttct tgcgaccgat cttcgagaag
     4501 ccgcttcggc cgcagcaggc tgccgatatg ctcggcgcgg aacgggggcg cgctcacggc
     4561 cgcacgagca atgccaccgg gaacgtcgcg aggatctgcg cgagaccgac gtgcccgccg
     4621 cgcgcctcgt gtcgctcgcc ggtcagcacg ttgaggtatt cgcccgccat cggcaggcgc
     4681 gactcccccc aggccttggc cccgagcggc gcgacgtcgc cgccctccac cagcctggaa
     4741 acgagccgcg gcgccacggc gatcaccgag cgcgcgccct cgcaaagcgc gaacgcgatg
     4801 acgttctcct ctcggccggc gtcggcgtag aggggcgtgt acgccgcacc gtggaacagc
     4861 gtcggcaacg ccttgcggac ctccaggcct ttccggatga cctgcatctt ggcgcggccg
     4921 tccgcaagat ttttcaggag gtcttctgaa ttcttcagca gcttctttcg caactcgtag
     4981 tccaccgggc gccggttgtc gggatcgacc aggctgaagt cccacagctc ggtgccctga
     5041 tagtagtccg gcacgcccgg ggacgcgacc ttcaccaggg cctgcgacag cccgacgagc
     5101 aggccgaggt gcacgacgcg cttcacggta ttgccgaaat cttccacgaa ggctttgttt
     5161 tccagcgagc gaatgacgag gcgctcgagc gcggcttcgt attcggcatc cggattgatc
     5221 cagctcgtat gttcctttgc ctcgcgcgcc gccttcagca tgtacgcctt caggcgctcg
     5281 gcgaggtcgc cggaaacctt gcctggccag atgccgatca gcgcctggta gaaatggtat
     5341 tcgtcgcttc gcgatatgtt gctttcattc atcccgctcc agcggcgaag cgtgatccgc
     5401 catcccgacg cgagctccga aagcacggcg aggcgcgctc gcacgtcttc cgagcgcttg
     5461 gtgtcgtgcg tcgagctgcc gagcatcgtg tagggccagc gccgcgcgcg ctcttcggct
     5521 gctgcgtgga attccttcaa cgggatgccg aagcgcgagg gatggccgcc gacctcgttc
     5581 agcgcgatca ggcggttgta gcggtagaac gccgtgtcct cgacgccctt ggccaccacc
     5641 ggcgcgctga actgctggaa gcgcatcgag aacctgagca tttcgctgcg tcgccggccc
     5701 gaaggcggag cggcgtccag ggtgaggacg ccgtgcacga agtcgaacac gctcgggtcg
     5761 gcgatgcggc tcgcgcgctt cgccgccctc accgcgccgc cgatcacgcg ccggtcgccg
     5821 tcggacacgc cgcgcgcgga aacataggtg cggtagaccg ggaactgcgc cgcgatctcg
     5881 gcgagcgctt tgcgcaggcc gctcgcggtg tagtcgcgcg tcgagcggtt gccggcggcg
     5941 atgcgcgcca accagttcga cagcatgtgc agctcggccg cgagcgtggt cgccatgatg
     6001 agccagcggc tctcgcgcgc gatctcctcg aagctgcgcc gctggccggt aaagctgcgg
     6061 tagacgcggt cgacgcgaga ttcggccgcg ccatcgatga acacgccggt caggaggttc
     6121 gcgaaccggt agccggtctc gccgtgcacc ggccagttct cgggcagctt ctcgtggtcg
     6181 gcgaggatct tctcgatcag gacccagcgc cgcgggaaca gctccgccag ccggtcgaag
     6241 tactgcttcg ggtcggcgag gccgtcggga tggtcgatgc gcacgccgtc gatgccgccg
     6301 gccttgacga gccgggcgac caggccatgc gtcgcctcga acaccgtgcg gtcctcgacc
     6361 ttcacgccgg cgaggtcggt gatctcgaag aagcgccggt aattgatttc gtcggaggcg
     6421 acgcgccagt acgcgagccg gtagtactgc ttctcgagca ggcgatggag cgcgagcgtg
     6481 tcccgtggcg gcgccttcag cccgcgctgg ctcttcgcgt tcagcgggaa gcgatggtcg
     6541 aaatagcgca cgctccactt gccgttcttc ttctcgaccg tcagctctgc gagcgcttcg
     6601 ccgtagtgct tgccgagcac cggcagcagc agcttgccgc gcgcccagtc gatatcgaag
     6661 aaccgggcgt agggcgatgc cctgcccttc tccagcacgt cgagccacca cgcgttgtcc
     6721 gcatggagca cgcccatgtg gttcggcacg aggtccagca ccattcccat gccgtgcttt
     6781 cttagtcttt gtattagttc atgcagctcg gcttcggtgc cgatctccgg attgacctgg
     6841 ccatggtcga tgacgtcgta gccgtgcatg cttcccgggc gcgccttgag aaagggcgac
     6901 gcatacaggt ggctgacgcc gagcgccgcc aggtacggca ccagggcggt ggcatcccgg
     6961 aatttgaatc ccttgtggaa ctgcaggcga taagtggccg ccggcaccac gagcggcgcg
     7021 cgcgtggcga cgctcctttc ggccatgatt ccggcaagcg ccgccacgcg cggctcggcg
     7081 ctccagcgct cgagcgcgag cggcagcttg cgccgccagt tcgggtgctc gtcgaccgtc
     7141 cctggaaggt tcgcctggtc gaccatctcg agtacgtcct cgggctgcag catggcgagc
     7201 ttcgccgggg tgcgggcgat gaacgcgtgc gccgagcgcg ccgagagatc tagcccttcg
     7261 cgctcgagcg tcgccttgag cttcctcttt tccgtttcgc gcagcgcgag ctctttcctc
     7321 gcgtcgacgg taagaccgag ccgctcccga agctgcagat cgtgcgcgtc ccagtagccg
     7381 cgccaggtcg gcaggtcgtg cgtggtgacg cagaccagcg cctcgcgggg atagtccttc
     7441 gatcgcttgt tctcgaagag aagcggccgg taggaaagga cacccgccgc gttgagcgcg
     7501 gcgcgcatct cagccgtcac ggtgcccagg tcctcgccga tcaccaggca gcgcgcgcgg
     7561 cggctctcgg cggcgaggat cgcga
//
LOCUS       DATBSZ010000175         4104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1120348, whole genome shotgun sequence.
ACCESSION   DATBSZ010000175 DATBSZ010000000
VERSION     DATBSZ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120348"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..222)
                     /locus_tag="VIG70_11405"
                     /pseudo
     CDS             complement(1..222)
                     /locus_tag="VIG70_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490499.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            498..1622
                     /locus_tag="VIG70_11410"
     CDS             498..1622
                     /locus_tag="VIG70_11410"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8251208.1"
                     /translation="MIRRFAATAVVVMLAAGAASAQQPATKTLKMQATWPTVVTAWDN
                     FQFLAERIDKLTAGSLKIQTLPAGQVVPAFETLDAVNRKVLDGGHSIAYFWSGKNKTA
                     VLFTGGPGGTYGMDMIDFIGWMYEGGGWELYQEFYTGVLKMNIIGFPTLPTTPQAFGW
                     FKRPIKNLADMKGMKCRQTGITAEVFQKMGMSTVNLPPGEIIPAAQRGVIDCAEYVGG
                     MDDLKLGFPGVWKYHYTPGMHESTSTGEIIFNLDVWKSLTPQQQEAVRSAANESFSRW
                     WAKWQKGQAEAMKEMREKHGVQILRTPPDILYAFLKAWDQVAAEESAKNPFFKKTLES
                     QKAYASLVVPAKRFLFPPYSFAANYYWPEEKPAAKSAKKQ"
     gene            1625..2578
                     /locus_tag="VIG70_11415"
     CDS             1625..2578
                     /locus_tag="VIG70_11415"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8251209.1"
                     /translation="MNKLAWLGAALLASSAALAQTYPAKPIRLVTPYSPGGAGDWVAR
                     TTTDRMAQALGQQFVIEPRPGAGGNIAAESVVRSSADGYTLLFATPLLAINATLYRKL
                     SFDATRDLVPVGVIATGPYVLYATGSLPVKNAGELVALAKQKPGSINYASLGIGTGPH
                     LGAALLGLMGGAEFTHVPYKGFGQALPDLASGQVHFSLNGIGVADAFLKDGRVKMLGF
                     TSAARMKEYPDVPTVSETVPGYEVLGWYGFAAPAGTPAAIVERLNAEVVKVTTSEFAS
                     QIEKRGLIPQSFSLAQTREFVARDSERWGRAVKASGAKIDD"
     gene            2601..3233
                     /locus_tag="VIG70_11420"
     CDS             2601..3233
                     /locus_tag="VIG70_11420"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8251210.1"
                     /translation="MNSAVDTKPAYAAMDAGDGKPSLRGIHHLAIVTEDMRGTLDFYV
                     RVLGMPLVHALTTSSNATSTHGRGAPPFRGIPHLFLDMGGDSLLAFFEYPKGGVGPSE
                     RDRIGAMQHVSFACGPKRFHEMVERVKAAGVQIVGGPVKVIDPAIHSFYFFDPVNGTR
                     LEIVSDFDGDEHGLRVVRSVDQSEATIRAELAKVSDDPAWIDEMIAARSR"
     gene            3241..4101
                     /locus_tag="VIG70_11425"
     CDS             3241..4101
                     /locus_tag="VIG70_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8251211.1"
                     /translation="MKLCRYAPDRVGVIRDASVHDVSAIADKLPRHGWPYPSGDQLIA
                     NLGTLRAEIEKLADRAKPRPLSEVELLAPVANPGKVVVAGTNYADHIEEAARDQTIAH
                     GRQVNRTLEEKGVFLKATSSIVGPSQGVAQRFVDRRNDQEVELCAVIGKAGTRIPGAR
                     ALEHVAGYMVGLDMTVRGTEHPSLRKSIDSYTVFGPCLATADDVGDPQDLELKCWVNG
                     EQRQGSSTRYMISGVARLIEYASLFYTLHPGDVLMTGTPAGVAPVKPGDVIEAEIERI
                     GRMRVAVRAG"
BASE COUNT          734 a         1413 c         1336 g          621 t
ORIGIN      
        1 ctgcgtccgc ttgtctcctc cgcaaaggag gaggatcacc acctgcccaa gccgggcgaa
       61 gtacatacga tatcccggcc cccaatcgat cctgagctcc atcacaccct cgccgacggt
      121 ctcggagtca ccgaagttcc ctgcgcgtag gcgcgcaagc cgagcttcga tgcgtatgac
      181 ggcttgcatg tctcttacgc cgcgcagcca ctcctggaat ggccttcagc gctcggcggt
      241 ctgatagaac tgaagctcca cggtaattgt aacttatagt taacaggtac gctagaggct
      301 ccgtaccgtt ccccagccct tgacgcgaat cactgattga acgcacgagg cgcgcgttcg
      361 atgacgaggc caaaaaaaag gccttggcgc gcgactaagg gccgctcgac cgagggattc
      421 gtttcaaatc aacggcgcga caccgccgtg tggcagaatc ggcgcgtgta tcgcgtctca
      481 acgggaggag gcccgaaatg atccgccgat tcgccgcaac cgccgtcgtg gtcatgctcg
      541 ccgccggcgc cgcatccgcg cagcaacccg ccaccaagac gctcaagatg caggccacgt
      601 ggccgacggt ggtcaccgcg tgggacaact tccagttcct cgccgagcgc atcgacaagc
      661 tcaccgccgg ctcgctcaag atccagaccc tgcccgccgg ccaggtcgtg ccggcgttcg
      721 agacgctcga cgcagtgaac cgcaaggtgc tcgacggcgg ccattccatc gcctacttct
      781 ggagcggcaa gaacaagacc gcggtgctgt tcaccggcgg cccgggcggc acctacggca
      841 tggacatgat cgatttcatc ggctggatgt acgagggcgg cggctgggag ctctaccagg
      901 agttctacac cggcgtgctg aagatgaaca tcatcgggtt cccgacgctg ccgaccacgc
      961 cgcaggcgtt cggctggttc aagcggccga tcaagaacct cgccgacatg aagggcatga
     1021 agtgccgcca gaccggaatc accgccgagg tgttccagaa gatgggcatg tccacggtga
     1081 acctgccgcc gggcgagatc atccccgccg cgcagcgcgg cgtcatcgac tgcgccgagt
     1141 acgtgggcgg catggacgac ctcaagctcg gcttccccgg cgtctggaaa taccactaca
     1201 cgcccggcat gcacgagtcg acctccaccg gcgagatcat cttcaacctc gacgtgtgga
     1261 agtcgctcac gccgcagcag caggaagcgg tccgctccgc ggcgaacgaa tcgttctccc
     1321 gctggtgggc caaatggcag aaaggccagg ccgaggcgat gaaggagatg cgcgagaagc
     1381 acggcgtgca gatcctgcgc accccgcccg acatcctcta cgcgttcctg aaagcctggg
     1441 accaggtggc ggccgaggag tcggcgaaga acccgttctt caagaagacg ctcgagtcgc
     1501 agaaggccta cgcctcgctc gtggtgccgg cgaaacggtt tcttttcccg ccctactctt
     1561 tcgccgccaa ctactactgg ccggaagaaa agcccgcggc gaaaagcgca aagaagcaat
     1621 agcgatgaat aagctcgcgt ggctcggcgc agcgctcctg gcctcgagcg ctgcgctcgc
     1681 acagacctat ccggccaagc ccatccggct ggtcacgccc tactctcccg gcggcgccgg
     1741 cgactgggtg gcgcgcacga cgaccgaccg catggcccag gcgctcggcc agcagttcgt
     1801 gatcgagccg cgtcccggcg cgggcggcaa catcgccgcc gaaagcgtgg tgcgttcgag
     1861 cgccgacggc tacacgctgc tctttgctac gccgctgctc gcgatcaacg ccacgctcta
     1921 tcgcaagctc tcgttcgatg cgacccggga cctggtcccc gtcggcgtca tcgcgaccgg
     1981 gccgtacgtg ctgtacgcga ccggcagcct gccggtgaag aacgccggcg agctcgtcgc
     2041 gctggcgaag cagaagcccg gctcgatcaa ctacgcctcg ctcggcatcg gcaccggccc
     2101 gcacctcggc gccgcgctcc tcggcctgat gggcggcgcg gagttcaccc atgtgcccta
     2161 caaaggcttc ggtcaggcgc tgccggacct cgcttcaggc caggtgcact tctccctgaa
     2221 cggcatcggc gtggccgacg ccttcctcaa ggacgggcgc gtcaagatgc tcggcttcac
     2281 ctcggctgcg cgcatgaagg agtatccgga cgttcccacc gtctccgaga cggtgcccgg
     2341 ctacgaggtg ctcggctggt acggcttcgc cgcgcccgcc ggcacgccgg cggcgatcgt
     2401 cgagcggctc aacgccgagg tggtcaaggt caccacttcg gagttcgcgt cgcagatcga
     2461 gaagcgcggg ctgatcccgc aatccttcag cctcgcccag acgcgcgagt tcgtcgcccg
     2521 cgattccgag cgctgggggc gcgcggtcaa ggcttccggc gccaagatcg acgattagaa
     2581 tcaggcagga ggaggaaccc atgaacagcg cagtcgacac caagcccgcg tatgccgcga
     2641 tggacgcggg cgacggcaag ccctccctgc ggggcatcca ccacctcgcg atcgtcaccg
     2701 aggacatgcg cggcaccctg gatttctacg tgcgcgttct gggcatgccg ctggtgcacg
     2761 ccctgaccac ctcgtcgaac gcgaccagca cccacggcag gggcgcgccg ccgttccgcg
     2821 gcatcccgca cctcttcctc gacatgggcg gcgacagcct gctggcgttc ttcgaatacc
     2881 cgaagggcgg ggtcggcccg tccgagcgcg accggatcgg ggcaatgcag cacgtgtcgt
     2941 tcgcctgcgg gccgaagcgc ttccacgaga tggtggagcg ggtcaaggcg gcgggcgtgc
     3001 agatcgtcgg cggcccggtg aaggtcatcg acccggcgat ccactcgttc tacttcttcg
     3061 acccggtcaa cggcacgcgg ctcgagatcg tttccgactt cgacggcgac gagcacggcc
     3121 tgcgcgtggt gcggagcgtc gatcagagcg aggcgacgat ccgcgcggag ctggcgaagg
     3181 tcagcgacga tccggcgtgg atcgacgaga tgatcgcggc gcgctcgcgc tagagcgcgc
     3241 atgaagcttt gccggtatgc gcccgaccga gtcggggtga tccgcgatgc ctccgtgcac
     3301 gacgtttccg ccatcgcaga caagctgccg cgccatggct ggccgtatcc aagcggcgac
     3361 cagctcatcg ccaacctcgg aaccttgcgc gcggagatcg agaagctcgc cgaccgcgcc
     3421 aagccgcggc cgctgagcga ggtcgagctg cttgccccgg tcgccaaccc cggcaaggtg
     3481 gtggtggcgg ggacgaacta cgccgaccac atcgaggagg cggcgcgcga ccagaccatc
     3541 gcccacggcc gccaggtaaa ccggacgctc gaggagaagg gcgtgttcct gaaggcgacc
     3601 tcgtcgatcg tcgggccctc gcagggcgtg gcgcagcggt tcgtcgaccg acgcaacgac
     3661 caggaagtcg agctttgcgc cgtgatcgga aaagccggca cccgcatccc gggcgcccgc
     3721 gcgctcgagc acgtcgccgg ctacatggtc ggcctcgaca tgacggtgcg cggcaccgag
     3781 cacccgtcgc tgcgcaagtc gatcgacagc tataccgtgt tcggtccctg cctcgccacc
     3841 gccgatgacg tgggcgatcc gcaggacctc gagctaaagt gctgggtgaa cggcgagcag
     3901 cgccagggct caagcacgcg ctacatgatc tccggcgtgg cgcgcctgat cgagtacgcg
     3961 agcctgttct acacgctgca tcccggcgac gtgctgatga ccggcacgcc ggccggcgtc
     4021 gccccggtga agcccggcga cgtgatcgag gcggaaatcg agcgcatcgg ccgcatgcgc
     4081 gtcgcggtgc gcgcgggcta ggga
//
LOCUS       DATBSZ010000176         8566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_711384, whole genome shotgun sequence.
ACCESSION   DATBSZ010000176 DATBSZ010000000
VERSION     DATBSZ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8566
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711384"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..2006)
                     /gene="ftsH"
                     /locus_tag="VIG70_11430"
     CDS             complement(126..2006)
                     /gene="ftsH"
                     /locus_tag="VIG70_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY8251212.1"
                     /translation="MLKNLVIWLVIGLVLMTVFNQFNTRQSAQAPMEYSQFLEEVKAG
                     RIAKVTIEGRQLKATTSEGKRVMSYSPGDLWLVSDLLKYGVKIDAKPEEEPSLLMNIF
                     VSWFPMLLLIGVWIFFMRQMQGGGRGGAFSFGKSRARMLDESTNTVTFGDVAGCEEAK
                     EEVAELVDFLRDPSKFQKLGGRIPRGVLMVGAPGTGKTLLARAIAGEAKVPFFSISGS
                     DFVEMFVGVGAARVRDMFEQAKKHAPCIVFIDEIDAVGRQRGAGLGGGNDEREQTLNQ
                     LLVEMDGFEATMGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREEILKVHM
                     RKVPLAPDVRPDIIARGCPGFSGADLANLVNEAALFAARGNKRLVDMDDFERAKDKII
                     MGAERRSMVMPEEERRNTAYHESGHAIVARLLPKTDPVHKVTIIPRGRALGVTMQLPT
                     EDRYSQDRDRLLSMIAVLFGGRIAEELFMHQMTTGASNDFERATELARRMVTQWGMSD
                     ELGTMVYGENEGEIFLGRSIQTHKNVSEATMQKVDAEIRRIIDQQYQVARKLLEDNRD
                     KAEAMTKALLEWETIDSDQISDIMEGKPPRPPKPSGGSQKPSKPASDGGAQGETAPAP
                     TA"
     gene            complement(2054..2644)
                     /locus_tag="VIG70_11435"
     CDS             complement(2054..2644)
                     /locus_tag="VIG70_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEY8251213.1"
                     /translation="MTKSSKEWLRRHVTDPYVKQAKKQGYRSRAAFKLLEIDAKEKLF
                     RPGQTVLDLGAAPGGWSQVAAAKVKPGGRVIAIDLLEIAPISGVAALKGDFRQVSIDA
                     RADVVLSDVSPNLSGIANVDQARLLELAEAAVELCRKVLKPDGVFVVKAFHGEAFDAL
                     IERLKAVFTKVKVVKPAASRGESAETYVVARGLPPL"
     gene            2726..4435
                     /locus_tag="VIG70_11440"
     CDS             2726..4435
                     /locus_tag="VIG70_11440"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY8251214.1"
                     /translation="MRLLGLLALLLPLHALAARVEIPLRLSLDTLRAALGAQLAATPY
                     RDGPCRYLKLGEPKLESIDGRLRLSAPGRGVLGVPSAGQCQAAVWRGTLHVTLVPQLD
                     GAGQLRLRIVDSRMVDARGGAAPLLWELGKRHIHPRLERFSYGVDTSLLRSFAPAMDP
                     LLRQIQFLPPRVASGHIDLPVAFDVPDAWLTAPPASSAPLTEAEMDALEKALEPWDAF
                     LVYVVRHVALAGGDEELRKRLFDLLIDSRHRLVELLSGDARAQGDPARALFLDTWSEL
                     REILAGRYALFADAADALAAFEAAAPGAPLSTGALRQLARGLAPGASGDPLAYDWNVD
                     PELGRVFQVEEIGEVDAEPAPPPATSWLDFLVRPARADGAPERWIPTREQLAAYETRV
                     GGLLRKTSAGELERTALPQPYGRMYRDLVPTTALIESCWRQYAMRGGKVSYLRSHSGS
                     VGIMQINQRVWRGFYDIERVRWDTAYNMRAGAQILLRYLTDYAIPYAEKTGKPEDAAR
                     AAYAVYNAGPRAVARFAKSPPHPREARVDEKLLTLYRGIASGEQVDLANCSVRSAVAL
                     KKP"
     gene            complement(4445..>8566)
                     /locus_tag="VIG70_11445"
     CDS             complement(4445..>8566)
                     /locus_tag="VIG70_11445"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8251215.1"
                     /translation="PDFHPVLRQWWASRFEEATEAQLEGWRAIRRGDDTLIAAPTGSG
                     KTLAAFLTALDELFREGVQQGGLPDEVRVIYVSPLKALSADIHKNLAVPRREIHALSP
                     VKITAAVRSGDTPQPERAAMLRTPPHILVTTPESLYLLLTAERSRAMLKTARVVIVDE
                     IHAVLQSRRGAHLALSLERLDHVCGRKLQRIGLSATQKPIEEVARFLTSSSCVVVNKG
                     HKRQVDLAVEVPPSPLEAVMSHEVWKEIYARLVGLVEAHRTTLITVNTRRLAERMAHQ
                     LSERLGADNVAAHHGSMAKDARLDAEEKLKNGKLKVLVATASLELGIDIGHVDLVCQI
                     SSPHRIATFLQRVGRSGHTIKGVPKGRIFPLTRDDLIECAAMVRAVHDGELDRVSVPD
                     KPLDVLAQQIVAETAAEVEWDEDALFNLFKGAYPYRNLEKQDFDEVVEMLARGYATRR
                     GRRGALIHHDSLNKRLRSRKGSRLNAIVNGGAIPEVFDYRVLLEPEGHFIGTLNEDFA
                     IESLPGDVFQLGNTSWRILRIGNGTVRVADAQGQPPSMPFWLGEAPARSDEMSAAASQ
                     LRAAVGFELPGPDEPRKQGELASAVELLKTEYLLSESAAEQIATYLAEAKRSLGTVPT
                     MDTVALERFFDESGGMQLVLHAPFGSRINRAWGLALRKKFCQGFNFELQAAATEEGII
                     LSLGGAHSFPLEEVLRYLHPGTVRETLMQALLQSPIFETRWRWSATLALAVPRNRGGA
                     RIPNQLQRMYAEDLLQAVFPDAVACQDNLPGAREIPDHPLVNQAMRDALEEAVDATGL
                     EKTLARLVNGEIGFVARDTPEPSVLSHELLNSAVYTFLDDAPLEERRTRAVYTRRATE
                     ARSADDLGALDPAAIERVRREAWPVANTADEMYDALMVAGYIKENELAPHWTDLLEQL
                     GERAVNRENAWFAHERLKDDELEITASRLEVLGPVTSPETAALLALEGQGRILRGKFS
                     STELQWCDRRLLARIHRYTLNKLRSEIEPVSAADFMRFLLHWQHVAGEDQVKGADGLM
                     AVVEQIEGFELAAAAWENDVLPARVADYEPEQIDRLCLSGRVAWGRLTPGSKAPLRSS
                     PIAIMPRAHLGHWGVEKHDAIELSSEARAVAEALETRGASFFHELASHARLLPSYVER
                     GLAELAGAGIATADSFAGLRALLAPQEKRKGMIETAGRWVLLSSTSQDEVEAIARALL
                     KRYGVVFRSLLQRESHLPPWRELVRVYRRLEARGEIRGGRFVAGFGGEQFAAPDAVGR
                     LRAVRKSEKTGELVVLSAADPLNLVGILTPEARVAAIYRSRVLFQDGLPIAAVEGGEV
                     RRLAQSDIDDARLRTLLARRSLRHPLRSHLRAPTPLESRRVVSARERLKVFRV"
BASE COUNT         1319 a         2984 c         2834 g         1429 t
ORIGIN      
        1 ggaaaaggaa tcgggcgtga cgttgaccac gcccatgatg aggggcttgt cgagcgcgag
       61 ccggaagcgt ccgcaccgca ggacgccgct cggactgctg ctcaggttac cttgttcgct
      121 cttctctatg cggtcggggc cggagcggtc tcgccttgcg cgccaccgtc actggcgggc
      181 ttggagggtt tctgcgagcc gccggagggc ttgggcggac gcggcggctt gccttccatg
      241 atgtcggaga tctggtcgga gtcgatcgtc tcccactcga gcagcgcctt ggtcatcgcc
      301 tccgccttgt cgcggttgtc ctccaggagc ttgcgcgcga cctggtactg ctggtcgatg
      361 atgcggcgga tctcggcgtc gaccttctgc atggtcgctt ccgacacgtt cttgtgcgtc
      421 tggatcgagc ggccgaggaa gatctcgccc tcgttctcgc cgtagaccat ggtgccgagc
      481 tcgtcggaca tgccccactg cgtcaccatg cggcgcgcga gctcggtggc ccgctcgaag
      541 tcgttcgagg cgccggtggt catctggtgc atgaacagct cttcggcgat acgcccgccg
      601 aacagcaccg cgatcatgct cagcagccgg tcgcggtcct ggctgtagcg gtcctcggtc
      661 ggcagctgca tcgtcacgcc gagcgcgcgg ccgcgcggaa tgatcgtcac cttgtgcacc
      721 ggatcggtct tcggcagcag gcgcgcgacg atcgcgtggc cggactcgtg gtaggctgtg
      781 ttgcggcgct cctcctcggg catgaccatc gagcggcgct cggcgcccat gatgatcttg
      841 tccttggcgc gctcgaagtc gtccatgtcg accaggcgct tgttgccgcg cgcggcgaac
      901 agcgccgcct cgttcaccag gttggcgagg tccgcgcccg aaaagcccgg gcagccgcgc
      961 gcaatgatgt cgggcctcac gtccggcgcc agcgggacct tacgcatgtg caccttcagg
     1021 atctcttcgc gcccgcggat atcgggcagc ggcacgacga cttggcggtc gaagcggccc
     1081 gggcgcagca gcgccgggtc gagcacgtcg ggccggttgg tggcggcgat cacgatcacg
     1141 cccatcgtcg cctcgaagcc gtccatttcc accagcagct ggttcagcgt ctgctcgcgc
     1201 tcgtcgttgc cgccgcccag gccggcgccg cgctggcggc cgaccgcgtc gatctcgtcg
     1261 atgaacacga tgcacggcgc gtgcttcttc gcctgctcga acatgtcgcg cacgcgcgcc
     1321 gcgccgacgc cgacgaacat ctcgacgaag tcggagcccg agatcgagaa gaacggcacc
     1381 ttcgcctcgc ccgcgatggc gcgcgcgagc agcgtcttgc cggtgcccgg cgcgccgacc
     1441 atcagcacgc cgcgcggaat gcgcccgccg agcttctgga acttggaagg atcgcgcagg
     1501 aagtcgacca gctcggccac ttcttccttc gcctcctcgc agccggcgac gtcgccgaag
     1561 gtgaccgtgt tggtggattc gtcgagcatg cgcgcgcgcg acttgccgaa cgagaacgcg
     1621 ccgccgcggc cgccgccctg catctggcgc atgaaaaaga tccacacgcc gattagcagc
     1681 agcatcggga accaggacac gaaaatgttc atgaggagcg aaggctcctc ttccggcttg
     1741 gcgtcgatct tgacgccgta cttgagcagg tcgctgacga gccagaggtc gcccggcgag
     1801 tagctcatga cgcgcttgcc ttcgctggtg gtcgccttga gctggcggcc ctcgatcgtg
     1861 accttggcga tgcggcccgc cttcacctcc tcgaggaact gcgaatactc catcggcgcc
     1921 tgcgccgact ggcgggtgtt gaactggttg aacacggtca tcagcaccag cccgataacg
     1981 agccagatga ccaggttctt gagcatgttg ttcaaacgcc gaatctcctt cctaggtcct
     2041 tcaaaaaacc atcttacagg gggggcaagc cgcgtgccac cacataagtc tctgccgatt
     2101 caccccgcga cgccgcgggc tttaccactt tcactttcgt gaaaaccgcc ttcaggcgct
     2161 cgatcagcgc atcgaaggct tcgccatgaa acgctttgac aacgaaaaca ccgtccggct
     2221 tcaacacctt gcggcagagc tcgacggccg cctcggccag ctccagcaag cgcgcctggt
     2281 cgacgttggc aattcccgac agattcggcg acacgtcgga gagcacgacg tccgctcgcg
     2341 catcgatcga aacctgtcgg aaatcgccct tcaaggccgc cacccccgag atgggggcga
     2401 tctcgagaag gtcaatggcg atcacccggc cgccgggctt cactttcgcg gccgcgacct
     2461 gcgaccagcc gccgggcgcc gcaccaagat cgagcacggt ctggccggga cggaacagtt
     2521 tctccttcgc gtcgatctcg agcagcttga aggccgcgcg cgagcgatag ccctgcttct
     2581 tcgcctgctt tacgtagggg tcggtgacgt ggcgccgcag ccactccttg ctcgatttcg
     2641 tcacacggca aagcttaccg tggtcacatc cgagcatccc ccagcggacg caggttcatg
     2701 acgcggggag tagcttggct gcggcatgcg cctcctcggg ctccttgcgc tcctcctccc
     2761 cttgcacgcg ctcgccgcgc gcgtggaaat ccccttgcgc ttgtccctgg acacgctgcg
     2821 cgcggcgctc ggcgcccagc tcgcggcgac gccgtaccgc gacggaccgt gccgctacct
     2881 caagctcggc gaaccgaagc tcgagtccat cgacggtcgc ctgcgactca gcgctccggg
     2941 ccggggcgtg ctcggggtgc cgagcgccgg ccagtgccag gcggccgtgt ggcgcggaac
     3001 cctgcacgtc acgcttgtgc cgcagctcga cggcgcgggc cagctgcgcc tgcggatcgt
     3061 cgactcccgg atggtcgacg cgcgcggcgg cgcggcgccg cttctatggg aactcggcaa
     3121 gcggcacatc catccacgcc tggagcgctt cagctacgga gtcgacacct cgctgctgcg
     3181 cagcttcgcg ccggcgatgg atccgctgct gcggcaaatc cagttcctcc cgccgcgcgt
     3241 tgcgtccggc cacatcgatc tccccgtcgc gttcgacgtc cccgacgcct ggcttaccgc
     3301 gccgcccgcg tccagcgcgc cgctgaccga agccgagatg gatgccctgg aaaaggcgct
     3361 cgagccctgg gacgcgttcc tcgtgtacgt cgtccggcac gtggcgctcg cgggcggcga
     3421 tgaggagctg cgcaagcggc tgtttgactt gctgatcgac agccgccacc ggctggtcga
     3481 gctgctttcg ggcgacgcgc gcgcgcaggg cgatcccgcg cgcgcgctgt tcctcgatac
     3541 gtggagcgag ctgcgcgaga tcctggcggg acgctatgcc ctgttcgccg atgccgcgga
     3601 tgccctcgcg gccttcgaag ccgcggcccc gggtgcgccg ctgtccaccg gcgcgttgcg
     3661 ccagctcgcg cgcggcctgg cgcccggcgc gagcggcgac ccgctcgcgt acgactggaa
     3721 cgtggacccc gagctcggcc gcgtcttcca ggtggaagag atcggcgaag tcgatgccga
     3781 gcccgcgccg ccgccggcga cgagctggct cgatttcctg gtgcgcccgg cccgagccga
     3841 tggcgcaccc gagcgctgga taccgacgcg cgagcagctc gcggcctacg agacccgggt
     3901 cggcggcctg ctgcgcaaga cctcggcagg cgagctcgag cgcaccgcgc tgccccagcc
     3961 gtacgggcgg atgtaccgcg acctggtccc gacgacggcg ctgatcgaga gctgctggcg
     4021 ccagtacgcg atgcgcggcg gcaaggtgag ctatctgcgc tcgcattcgg gaagcgtcgg
     4081 catcatgcag atcaaccagc gcgtctggcg cggcttctac gacatcgagc gcgtgcgctg
     4141 ggacaccgcc tacaacatgc gcgccggcgc gcagatcctg ctgcgctacc tgacggacta
     4201 cgccattccc tacgcggaaa aaaccggcaa gcccgaggac gccgcccgcg ccgcgtacgc
     4261 ggtctacaac gccggcccgc gcgcggtcgc gcgcttcgcc aagtcgccgc cgcacccgcg
     4321 cgaagcgcgc gtggacgaga agctattgac gctctaccgc ggcatcgcga gcggcgagca
     4381 agtcgatctt gcgaactgca gtgtcaggag cgcggtagcg ctgaaaaagc catagcagac
     4441 cgacctacac ccgaaacacc ttcaggcgct cgcgcgcgct gaccacccgc cgcgactcga
     4501 gcggcgtcgg cgcgcgcagg tgcgagcgca gcggatggcg cagcgagcgg cgcgccaaca
     4561 gggttctcag tcttgcatcg tcgatatcgg attgcgcaag ccggcgcacc tccccgccct
     4621 ccaccgccgc aatcggcagg ccgtcctgga acagcacgcg gctgcggtag atcgccgcca
     4681 cgcgcgcctc gggcgtgagg atgccgacca ggttcagcgg atcggccgcg ctcagcacga
     4741 cgagctcgcc cgtcttctcg ctcttgcgca ccgcgcgcag ccgtccgacg gcgtccggcg
     4801 ccgcgaactg ctcgccgcca aaaccggcga cgaagcgccc gccgcggatt tcgccgcgcg
     4861 cttccaggcg ccggtagacg cgaacgagct cgcgccacgg cgggagatgg gactcccgtt
     4921 gcaataaaga acgaaaaacc acgccgtagc gcttgagcaa tgcgcgcgcg atggcttcca
     4981 cctcatcttg ggaagttgaa gagagcaaca cccaacgtcc ggccgtttcg atcatgccct
     5041 tgcgcttctc ctgcggcgcg agcagcgccc gtaatccggc gaaactgtcg gcagtcgcga
     5101 tgccggcgcc ggctagctcg gccagaccgc gctccacgta gctcggcaac agccttgcat
     5161 gacttgccag ctcgtggaaa aacgaggcgc cgcgcgtttc cagcgcctcg gccactgccc
     5221 tcgcctccga agacaattcg atggcatcgt gcttctcgac gccccagtgg ccgaggtgcg
     5281 cgcgcggcat gatggcgatt ggcgagctgc gcagcggcgc cttcgagccg ggcgtgagcc
     5341 ggccccaggc cacgcggccg ctcaggcaca ggcggtcgat ctgctccggc tcgtagtcgg
     5401 cgacgcgcgc cggtagaacg tcgttctccc aggccgcggc ggcgagttcg aagccttcga
     5461 tctgctcgac caccgccatc aagccgtcgg cgcccttcac ctggtcttcg cccgccacgt
     5521 gctgccagtg cagcaggaag cgcatgaagt cggcggcgct gacaggctcg atttccgacc
     5581 tgagcttgtt gagggtgtag cggtggatcc gcgccagcag ccggcggtcg caccactgca
     5641 gctcggtgga cgagaacttg ccgcgcagga ttcttccctg cccttcgagc gcgagcagcg
     5701 ccgcggtttc gggcgaggta accggtccga gcacttcgag ccggctcgcc gtgatctcca
     5761 gctcgtcgtc cttcagccgc tcgtgggcga accaggcgtt ttcacgattc accgcccttt
     5821 cgccgagctg ctccagcagg tctgtccagt ggggggcgag ctcgttctct ttgatgtagc
     5881 ccgccaccat cagcgcgtcg tacatctcgt ccgcggtgtt cgcgaccggc catgcttccc
     5941 ggcgcacgcg ctcgatcgcc gcggggtcca gcgcgccgag gtcgtccgcg ctgcgcgctt
     6001 ccgtcgcgcg ccgcgtgtag accgcgcgcg tacggcgctc ttccagcggc gcatcgtcga
     6061 ggaaggtgta caccgcggaa ttcagcagct cgtggctgag caccgagggc tcgggcgtgt
     6121 cgcgcgcgac aaagccgatt tcgccgttca ccaagcgagc taaggttttt tccaaacctg
     6181 ttgcatctac cgcctcctcg agcgcatcac gcatggcctg gttgacgagc ggatggtccg
     6241 ggatctcgcg cgcgcccggg aggttgtcct ggcaggccac cgcgtcgggg aacacggcct
     6301 gcagcagatc ctcggcgtac atgcgctgga gctggttcgg gatgcgcgcg ccgccgcggt
     6361 tgcgcgggac tgcgagcgcg agcgtggcgc tccagcgcca gcgcgtctcg aagatcggcg
     6421 attgcaggag cgcctgcatc agcgtctcgc gcaccgtgcc cgggtgcagg tagcgcagca
     6481 cctcttccag cgggaacgag tgcgccccgc cgagcgacag gatgattccc tcctccgtcg
     6541 ccgccgcctg cagctcgaag ttgaagccct ggcagaactt cttgcgcaac gcgaggcccc
     6601 aagcgcggtt gatgcggctg ccgaagggcg cgtgcaacac gagctgcatg ccgcccgact
     6661 cgtcgaagaa gcgctccagc gccaccgtgt ccatggtcgg cacggtgccg aggctgcgct
     6721 tggcttcggc caagtacgtg gcaatctgct cggccgcact ctcgctcagc aagtactctg
     6781 ttttcagaag ctcaacggca gaggcaagtt cgccctgctt gcgcggctcg tccggtccgg
     6841 gcagctcgaa gcccaccgcg gcgcggagct gcgacgccgc ggcgctcatc tcgtcgctgc
     6901 gcgccggcgc ctcgcccagc cagaacggca tcgacggcgg ctggccctgg gcgtcggcca
     6961 cgcgcacggt gccgttgccg atccgcagga tgcgccacga ggtgttgccg agctggaaca
     7021 cgtcgcccgg cagagattcg atcgcgaagt cctcgttcag cgtgccgatg aaatgccctt
     7081 cgggctcgag cagcacgcga tagtcgaaca cttccgggat cgcgccgccg ttcacgatcg
     7141 cgttcaggcg cgagcctttc cggcttctca gtctcttgtt caggctgtcg tggtgaatca
     7201 gcgcgccgcg ccggccgcga cgcgtggcgt agccgcgggc gagcatctcc acgacctcgt
     7261 caaagtcctg cttttcgaga ttcctgtacg gataggcgcc cttgaagaga ttgaaaagcg
     7321 cgtcctcatc ccattccact tccgcggcgg tctcggccac gatctgctgg gcgaggacgt
     7381 cgagcggctt gtcgggcacg ctcacgcggt cgagctcgcc gtcgtggacc gcgcgcacca
     7441 tcgccgcgca ctcgatgaga tcgtcgcgcg tcagcggaaa gatgcgccct ttcggcacgc
     7501 ccttgatggt gtggccggag cggccgacgc gctgcaggaa ggtggcgatg cggtgcggcg
     7561 aagagatctg gcacacgagg tcgacgtggc cgatgtcgat gccgagctcg agcgaagccg
     7621 tcgcgaccag caccttcaat tttccattct tcagtttttc ttccgcatcc aggcgggcgt
     7681 ctttcgccat gctgccgtgg tgcgcggcga cgttgtcggc gcccaggcgc tcggaaagct
     7741 gatgcgccat gcgctcggcc agccgccgcg tgttgacggt gatgagggtg gtgcgatgcg
     7801 cttcgaccag gccgaccagc cgggcgtaga tctccttcca cacctcgtgg gacatcactg
     7861 cttcgagcgg cgacggcggc acttcgaccg cgagatccac ctggcgcttg tggcccttgt
     7921 tgacgacaac acaagaggaa gaagtcaaaa acctggcgac ttcctcgatc ggtttctgcg
     7981 tagccgaaag cccgatccgt tgcagctttc gcccgcagac atggtcgagg cgctcgagcg
     8041 aaagcgcgag gtgcgcgccg cggcgcgact gcagcacggc gtggatctcg tcgacgatca
     8101 ccacccgcgc ggtcttcagc atggcgcgcg agcgctccgc ggtgagcagc aggtagagcg
     8161 actcgggcgt cgtcaccagg atgtgcggcg gcgtgcgcag catcgccgcg cgctcgggct
     8221 gcggggtatc gccgctgcga accgccgccg tgatcttcac cggcgacagc gcatggatct
     8281 cccgccgcgg cacggccagg ttcttgtgga tatcggccga gagcgccttc aggggcgaga
     8341 catagatgac ccggacttca tccgggagac cgccttgctg gacgccttca cggaacaatt
     8401 cgtccaaagc ggtgaggaag gcggccagcg tcttgccgga gccggtcggc gccgcgatca
     8461 gcgtgtcgtc gccgcgccga atggcgcgcc agccctctag ctgtgcttca gttgcttctt
     8521 cgaaccggct ggcccaccac tggcggagga ccggatgaaa atcggg
//
LOCUS       DATBSZ010000177        19971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1394638, whole genome shotgun sequence.
ACCESSION   DATBSZ010000177 DATBSZ010000000
VERSION     DATBSZ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19971
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394638"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..333)
                     /locus_tag="VIG70_11450"
     tRNA            complement(258..333)
                     /locus_tag="VIG70_11450"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(298..300),aa:Thr,seq:cgt)
     gene            503..1012
                     /locus_tag="VIG70_11455"
     CDS             503..1012
                     /locus_tag="VIG70_11455"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HEY8251216.1"
                     /translation="MRRVGGFTLIEIMVTVAVLAVLLVAAAPAMTDMVLNNRMAGLSN
                     DLMSDLAIARSEALKRGQRVVLCKNAGNDSSCGTGLWANGWLLYIDTDADTTLDGGET
                     ILRVRQALPAGYTVTPTGIMETLVVRPVGLVTPTGTFKICDQRTGNFGRLITISAGGR
                     ASSAPATCP"
     gene            1015..1554
                     /gene="pilV"
                     /locus_tag="VIG70_11460"
     CDS             1015..1554
                     /gene="pilV"
                     /locus_tag="VIG70_11460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEY8251217.1"
                     /translation="MLPTFLQRTPPARSDGVTLIEVLVAILILSFGLLGSAGLQMAGL
                     RAALAANQRTTATLLAYDIADRMRANMTGVIAGNYHGYTATQDVNCLQAAGCTAQQMA
                     QHDMWEWAQAIATQLPSGIGIVCRDGTPNDGTSNVSLVTANCDSLGNQYAIKIWWLED
                     RSETNPTGTLKRFTTAIRP"
     gene            1551..2339
                     /locus_tag="VIG70_11465"
     CDS             1551..2339
                     /locus_tag="VIG70_11465"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEY8251218.1"
                     /translation="MNQRARQAGFTLIEMMVAMALGLLLVVVIGQVFVTSKESYRTTE
                     DLSRLQENARFSAAQMSRVIRMAAFVTDTNNPVAARGVMFPSATAPALTGADGLAGAP
                     DQVTVRYEGSGAGTADGTVLDCQGNAIRAGDMAVARFYLAAGANGASALFCDNAGTVG
                     PGIVPAAVELASDVENMQILYGEDTDADGTANRYVERTTVPALDYERVVSVRVAILVR
                     STNQVAASVNTSTYNLLGTVFDPVDDRRLRRMYTSTITLRNRAP"
     gene            2336..2872
                     /locus_tag="VIG70_11470"
     CDS             2336..2872
                     /locus_tag="VIG70_11470"
                     /inference="COORDINATES: protein motif:HMM:NF025061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HEY8251219.1"
                     /translation="MKRLPLRTQRGTALIIAMLFLVILGMLGVTTMTATTLEERMAGN
                     TRDRDIAMQAAEAALRDAERDLTNTVAGSGRVVTLAMFSAGCTGGLCTEGVAIFTNID
                     DAAKSAYFTQFANPAQALQGTAQQPRYIIELLTAMPPQVPAPPVGQQIRNFRITAKGF
                     GRNANTIVMLQTVFQLTI"
     gene            2880..8027
                     /locus_tag="VIG70_11475"
     CDS             2880..8027
                     /locus_tag="VIG70_11475"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HEY8251220.1"
                     /translation="MKTSIRTIALALAASAAASTAHGAGLTLGTTPLYLGSSVAPIVM
                     LSVSKDEQLFQKLYNDYTDLDGDGLLDITYKHSINYYGYFDGFKCYNYDTALSRFVPI
                     ALSPASNKYCDTVAAGRWSGNFLNWATMTRADSVRKILYGGHRSTDTAALTVLERANI
                     PFDTHAFAKYYHGADINRLTPFTTTVTTPTTSAATGTDALYTGAIAQPISAGTKHFRN
                     IGVAVCKGDQVKVNLGAATTVNYVVGYVSAVNGVGDCSTTGGTSGFSIVVKTQGVSGA
                     GAGSNAGGAYTLTNLGSTGITICNVTDAGSTNLSHTTTNPPLIKVAKGNFAMWANNDI
                     TQCQWNPANGTRTFGGTGSNGNEGSLSGGLPTLFPAGSVNWSSAHEPIQATHGLAVGG
                     VFNFVARIEACKAGVLGFERCKAYGAGFKPIGLLQFYGESDLLYFGLMTGSYAKNKSG
                     GVLRKNAGTFTNEVNPANGTFLVPAGGGIVDTLNRLRIYGYDYATTSYGGAAPDRDGC
                     GVVTSGSGTAALNRGDAQEGLCTSWGNPVAETYLESLRYLAGKTVDPTFTYAAGSKDD
                     QVGGAPGLPKPAWVDPMSDTTKYCRPLNVLVFSANVTSFDNDQLGNVTQLNTASSAVT
                     LTNAVGTAETINGNNFFVGNDGAGNNNELCTLKNAISFGSVYGVCPESPRGRGSYLIS
                     GIAHHAHTNRIRVAPDPTALAVPPTNTRALKVNTYGITLAANSGTVQIPVGTNTVTLL
                     PIARVQDIIVGAGSGGLIDFKVIQQDATSGKFFVQWEVSQQGADRDLDLWGVISYTIN
                     TGVTPNTITVTTDVISANPSGFLGFGYVIAGTNQDGAHFHSGYNTFNYTYNPGGIVEC
                     TNCQTADAPTSKTYNVQNSTAQILKDPLYYAAKYGGFNDVNANSLPDQTIEWDSKKID
                     GSDGTDGIPDNYFPVTNPAALEAALDRAFIYILQVSSASSVATNSTSLTTGSRVYQAR
                     FNSNEWSGQLLSFTILPTGVIDPTPNFDAGQLLPASNSRIITTYSPRAGAPGVSNIPA
                     GVPFRWGSLQAAQQTALRKDGSSQIVDSLVGGACTSDNVPVGCTDRGSLRLEWLRGNP
                     VAEGTTATALRVRPSTKLADIVNSTPRFVGPPSASFTDPNYLAFRSFYAGRTPMLYVG
                     SNGGMFHAFEATTGAGGGIERFAYVPGKMYPNLTRLTTKPYSHRYFVDGTPSVTDACF
                     GACGAMNDWKTIAVGGYNSGGQGYYAINVTDPNSWSTEAGAAASVLWEFTDVNDPDLG
                     FSYSKPLIVKMSNGRWAAVFGNGYNNSIAESGELACDIGDGSAGNPYGRADAQPGCST
                     SLSGHAYLYILFLDGGTDGTWTQGVDYIKINTNTGTDTATPSNLTPGTPNGIATPVSV
                     DFNGDGMVDYIYAGDLVGNLWKFDVTSSSPGGWGVASAGAPLFIAQDASGNRQPITSA
                     PTLFPHTSGGFMVLFGTGKYMEITDDTGPFNMSAFYGIWDKLDNSTVTRSELMQQKVL
                     NVSAGNLDGGLVVGTNKVRLTSAYVPNYSGADRTNTVFGDADTDPESQAPTAITPANQ
                     RGWFLEMPNSGNGGPPNAPATGTGEKIVFDPLISTGKIVFTTLLPSTQPCDAGGTSFI
                     MDMDPVSGSRLSFSPFDLNGDSNFSSADFVSYGGKIIAVSGLGSTIGIVPQPTVIQGP
                     AGGGMEIKVLSGSSGGLQTVLENAPEPTPTPGARTSRRLIWRELFSN"
     gene            8062..8511
                     /locus_tag="VIG70_11480"
     CDS             8062..8511
                     /locus_tag="VIG70_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEY8251221.1"
                     /translation="MANLHARRGRGFTLIEVMIVVAIIGFLAAIAYPAYTNHIRKGKR
                     AEAKARLLLVSQRLERAYTDNGTYVTDVAPLVGLTAGATVYSADNNDAYSGYRITVAP
                     AAGGINTGYTITATLNAGFTDDECGNLTLTSTGVKGKTGSAPISRCW"
     gene            8508..9101
                     /locus_tag="VIG70_11485"
     CDS             8508..9101
                     /locus_tag="VIG70_11485"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY8251222.1"
                     /translation="MSPERRLAFSVAASSGLHLGLLAGAASLLSPPNDHFLRDAHRGP
                     SLEVTLIAAAPPALVIDAALPPMADAVRVLSKRAAPREKPGAEREAPPAKPETVYYSA
                     REVDVRAVPIGDIVPINPDLSGREAGMVVLRLLINAAGNVDNVLVLKAEPASSFGPQV
                     LLPFKQARFLPARKAGIAVNSEMLIELRYGALETTKP"
     gene            complement(9098..10030)
                     /gene="ispH"
                     /locus_tag="VIG70_11490"
                     /gene_synonym="lytB"
     CDS             complement(9098..10030)
                     /gene="ispH"
                     /locus_tag="VIG70_11490"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY8251223.1"
                     /translation="MEVLLANPRGFCAGVERAIETVQRALELHGAPIYVRHEIVHNKY
                     VVDDLRAKGAVFVDELDEVPPGGTVIFSAHGVSQQVRADADRRGLKVFDATCPLVTKV
                     HVEVARMLKAGYQIVMIGHRGHPETEGTMGQAVRGMYLVETVADVAGLQVGTPDKLAY
                     VTQTTLSIDDAKAIVAALKARFPAIRGPKKDDICYATQNRQDAVKLMAPQCDVVIVVG
                     SPNSSNSNRLREVAQHMGAQAYMVDRAADLRPEWIAGKRRVGVTAGASAPQVLVDELL
                     ARLKALGAQNVRVLPGITERVVFTLPRELVRAAR"
     gene            complement(10035..10553)
                     /locus_tag="VIG70_11495"
     CDS             complement(10035..10553)
                     /locus_tag="VIG70_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8251224.1"
                     /translation="MALLLKALAFAAHKHRDQRRKDHEASPYINHPIALADVLVNEGG
                     VSDVEVLCAALLHDTVEDTATTPEELVDTFGARVARIVAEVTDDKALNKADRKRLQVE
                     NAARLSPEAKLVKLADKICNLRDVAERPPAGWDLARRREYFDWAKRVVDGLRGSHPRL
                     EAAFDVAYGMRK"
     gene            complement(10537..11031)
                     /gene="lspA"
                     /locus_tag="VIG70_11500"
     CDS             complement(10537..11031)
                     /gene="lspA"
                     /locus_tag="VIG70_11500"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY8251225.1"
                     /translation="MSSAWRWFALAGAIVVADQLAKWAVLGYFADRAPREAITGFFNL
                     VLVCNKGAAFSFLADAPGWQTPLFIVFALAAAVLCGTLIVRNPGKRLFCAALGLIVGG
                     ALGNVIDRARFGCVVDYLDFYILERWPHWPAFNVADSAITVGAVLLILDGFVHHEKRA
                     GAPS"
     gene            complement(11024..13792)
                     /gene="ileS"
                     /locus_tag="VIG70_11505"
     CDS             complement(11024..13792)
                     /gene="ileS"
                     /locus_tag="VIG70_11505"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY8251226.1"
                     /translation="MADYKDTLNLPETPFPMRGDLAKREPVWVKEWHDKGVYRRLRAL
                     AKGRPRFVLHDGPPYANGDIHIGTAVNKILKDMVVKSKTLAGFDAPYVPGWDCHGMPI
                     EVQIEKKHGKNLPTDETLRLCRAYATEQIDRQRTQFQRLGVLGEWDDPYLTMAYRNEA
                     EEIRVLGRLLERGYVYRGLKPVNWCFDCGSALAEAEVEYEDRKDPAIDVGFAFAEPEK
                     VAKVFSTKVTKPGFAVIWTTTPWTLPGNQALNVHPELSYDLVDTDKGLLILASELREA
                     CLKRYGLSGKTVGTCKGKALEKIRFRHPFYEREAPVYLGEYVSLDAGTGIVHSAPAYG
                     VEDFESCRRYGMKDDEILTPVMGDGKFAASLPLFGGLSIWDANPKIVKTIEEKGALFA
                     VDWKHTHSYMHCWRHKTPVILRATTQWFASMDKPVKGESLRATALRGIENTQFFPAWG
                     QARLHGMIANRPDWTLSRQRQWGVPMPFFLHRETGALHPRTPELLEKVAQRVEQGGIE
                     AWQSVTAQELLGSESAQYEKSRDTLDVWFDSGSTFQTVLRGSHRSQSTYPAEMYLEGS
                     DQHRGWFHSSLLISCMTDGAPPYKSLLTHGFVIDMEGRKMSKSKGNTMAPQEIASTLG
                     AEILRLWVASADYSGELTISSEILKRVVESYRRVRNTLRFLLANTSDFDPAKHAVAPA
                     DMVEIDRYALAMTAAMQDEVTADYTRYQFHLVAQKLQAFCSEDLGAFYLDVLKDRLYT
                     CRADSAARRSAQTALWHVTHSLVRLMAPILSFTAEELWQLFPGKKDDSVFFHTWHEVP
                     APVDRAALLAKWGRLRELRTPVRKQIEDLRAAGKVGSSLQAEVDLTVPSSDYDLLGSL
                     AGELKFLMMTSAARVARGDALAANVVPSGNPKCERCWHYTADVNGEGLCARCQTNLRG
                     PGEKRKYV"
     gene            complement(13785..14702)
                     /locus_tag="VIG70_11510"
     CDS             complement(13785..14702)
                     /locus_tag="VIG70_11510"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY8251227.1"
                     /translation="MLTESMVVTHGSLQRARGRCALTIGNFDGVHRGHRALIDRVTAR
                     AREAKLTSCILTFEPHPREFFAADAAPARLTRLRDKLELMAQAGVDRVHVARFDAKFA
                     ALPAERFIEEVLVRGLAVQWLLVGRDFRFGARRAGSFATLQNQGFALETMPDVLFDGK
                     RVSSSAVRAALAAGNLAEGERLLGHPYTISGRVAHGAKLGKNLGFPTANIVLRRPPPI
                     AGIFVVEVDRYGRGVASVGRRPTVNPVAIPLLEVHLFDHYGDFYGEHLRVRFLKKLRD
                     EEKYDGLPALQAAIARDARQAKEYFLEHG"
     gene            14722..15297
                     /locus_tag="VIG70_11515"
     CDS             14722..15297
                     /locus_tag="VIG70_11515"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEY8251228.1"
                     /translation="MKNIEEQMSIYAAYHQDARNKVTHFIGVPAIILSLFIPLAWIRF
                     DAGGVTITAAMLFAAVVVLYYFFLDVALALAMLAVTGALVWLGHQIAQLGTAQGWIWF
                     ALLFVGGWILQLVGHVFEGRKPALADNLFQIFVAPIFLAAEVFFALGYKPQLHRRIQE
                     RAQFSRAQSADSSNNLPRTSSRSGASGSRSA"
     gene            complement(15207..16061)
                     /locus_tag="VIG70_11520"
     CDS             complement(15207..16061)
                     /locus_tag="VIG70_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8251229.1"
                     /translation="MGVALEAFSALIARDDARIDLARACLMIAEDAYPGLDVDRYMGE
                     IERMAIRLRGRLPKTGGAEERVVALNQFLYEDLGYWGNTDDYYDPRNSYLNEVIERKT
                     GIPITMSILYMELGRRIGLPLEGVSFPGHFLVRLRLRGGTLVLDPFAGGAPQSEAELR
                     SRVKRVIPDGVADDLPAAELPLDQFLEPATNRQILSRLLRNLKGIYRERDKPERMLDV
                     LNRMLLVTPDASAELRDRGYVYQRLECYRAALKDLTDYAEREPDAPDLDEVRGKLFEL
                     SALCARLN"
     gene            complement(16061..17599)
                     /gene="murJ"
                     /locus_tag="VIG70_11525"
     CDS             complement(16061..17599)
                     /gene="murJ"
                     /locus_tag="VIG70_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY8251230.1"
                     /translation="MNLLRILATVSSLTMVSRVLGYVRDFFIARVFGAGLATDAFFVA
                     FKIPNLLRRLFAEGAFSQAFVPILAEYKNRTSPEETKSLIDSITTVLFLALVLAAALG
                     MAAAPLIVYLTAPGFAAEPEKFSLTVGLLRITFPYIVFISLVALAAGVLNTWNRFSVP
                     AITPALLNVAFIVGAAFLAEYFDPPVMVLAWAVFAGGLLQLAFQIPFLMKMKLLPRWR
                     LDFSHPGLRRVLLLMAPAAFGVSVSQVSLLLNQIFASFLPTGSVSWLYYADRLMELPA
                     GVLGVAVGTILLPSLSKYHAAANTTEYSKLLDWGLRITVLLAVPSAVALAVLALPLIA
                     MLFHYGRFSAEDAWMTRQALVAYSLGLVGMILVKILAPGFYARQDVATPVKIGIFTLV
                     ATQLMNLFLVGPLRHAGLALAIGLGACLNALLLYFMLKRAKIYLPQPGWPVFILKILA
                     SVAFMAIVLFTTMGEARWWLQAGWQLKVPAILGLVLLGTAAYAGCLFAFGFRPRDFSR
                     RAAE"
     gene            17615..17878
                     /gene="rpsT"
                     /locus_tag="VIG70_11530"
     CDS             17615..17878
                     /gene="rpsT"
                     /locus_tag="VIG70_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY8251231.1"
                     /translation="MANIKSARKRAKQAVERRVHNMSLRTEVRTAMKNVKKAVAAGNK
                     DTAAKALRESQRVIDRIVAKGVLHRNAGDRHKSRLAHALKGMK"
     gene            complement(17915..18259)
                     /locus_tag="VIG70_11535"
     CDS             complement(17915..18259)
                     /locus_tag="VIG70_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HEY8251232.1"
                     /translation="MAEEAVVEFVIQGITLDGRPFRPSDWAERLCGVMAAFGGDRRGG
                     DHRMQYSPFVHPITANGVRCVVVDVRLEQIEPMAYRFLVNFAKDNELRVRDGRVAERT
                     RLAEWQRVGDTA"
     gene            18284..>19971
                     /locus_tag="VIG70_11540"
     CDS             18284..>19971
                     /locus_tag="VIG70_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8251233.1"
                     /translation="MTKPSSRILVINDEHLVLREFVKGLNAAARTLDNPLGISFAGVN
                     TAKEALRVIEADGNLQAVLVDDTLYTLGSNSGSHSKKKLQMSALELVQRITRFRPELD
                     VYIVIAQEKEDDVVDALFAESVDGYFYREERDYRGIYRILNAQIQERARTPFYDQLKN
                     YVWMAKDQWHTPGHSSGESLRGSPWVNDFYEFMGEHVFDADLSVSVPMLDSLMEPTGV
                     IGEAQAMAAKAFGARRTFFATNGTSTANKVIFQTLLAPGEKLLLDRNCHKSVHHGVVL
                     SGAHPVYLDSALNRKYGLYGPVPKKTLLREIKKHPDAQALILTSCTYDGLRYDLAPIV
                     EAAHAKGIKVIIDEAWYGFARFHPAFRPTALEAGADYATQSTHKVLSAFSQASMIHVN
                     DPGFREHLFRENFNMHTSTSPQYGLIASLDVARKQAVMEGYKLLGRTLELAAEVKKLI
                     NSTKVFRVLELDDLLPDAVKHDGIRLDPTKVTIDISGCGYTVEDLQKALFERFNIQVE
                     KSTFNTLTLLLTIGTTRSKVSRLYDALMRIAREQRAPRRLVQTPEIPPFTKLVFL"
BASE COUNT         3597 a         6894 c         6375 g         3105 t
ORIGIN      
        1 aagtgcgcac gcttgctgac ctagcggcct ccggaaaggc cgtttgtcgc acgccgtcgt
       61 cgccgcggtg aaggacgtca cgcgcggcga cgaactttgt catgcgacga aaactgtcag
      121 acaagaactg tcagtgacca acgattgtga aaaagtaaga acggcaacga gaagcagcag
      181 gtgttggtgc gagacgaagc gcgaacaaca gcggccccac acacggcggc cgcgtgtcgc
      241 agttcctcaa ttggatttgg tgctgctggt ccgactcgaa cggacgacct gacgcttacg
      301 aagcgactgc tctaccaact gagctacagc agctgactgc agggcatgga ttatagcggc
      361 agcgcgcagg acgcgtgaac tagttagcgt tgcgcgcgcc cgcgggcgcc tttcgctccg
      421 ccgtttccgc cgccggtcgt ccccttttgc gccacctgcg ggcccgtccg tagtatggcc
      481 ggaacccaaa agggggtctg ctttgcgcag ggtcggagga ttcacgctga tcgagatcat
      541 ggtcaccgtc gcggtgctcg ccgtgctgct tgtcgcggcc gcgccggcga tgaccgacat
      601 ggtcctcaac aaccgcatgg cgggtctcag caacgacctg atgtccgacc tggcgatcgc
      661 gcgaagcgaa gcgctgaagc gcggccagcg cgtcgtcctg tgcaagaacg ccggcaacga
      721 ctccagctgc ggcaccggcc tctgggccaa cggctggctg ctgtacatcg acaccgacgc
      781 cgacaccacg ctcgacggcg gggaaaccat tctgcgcgtg cgccaggcgc tgcccgccgg
      841 gtacacggtc acgccgacgg gaatcatgga aaccctggtg gtgcgcccgg tcggactggt
      901 cacgccgacg ggaaccttca agatctgcga ccagcgcacc ggcaacttcg gacgcctgat
      961 caccatctcc gctggcggcc gcgccagctc cgccccggca acctgcccat gaacatgctc
     1021 cccaccttcc tgcaaaggac gccgccggcc cgctcggacg gcgtcacgct gatcgaggtc
     1081 ctggtcgcga tcctgatcct ttccttcggg ctgctcggca gcgccgggct tcagatggca
     1141 gggctgaggg cagcgctcgc cgcgaaccag cggaccacag cgactctgct tgcctacgac
     1201 atcgcggatc gcatgcgcgc caacatgacg ggagtcattg cgggcaacta ccacggttat
     1261 acggcgacgc aggacgtgaa ctgcctgcaa gccgcaggct gcaccgcgca gcagatggcg
     1321 cagcacgaca tgtgggagtg ggctcaggcg atcgcgacgc agctgccttc gggcatcggg
     1381 atcgtatgcc gggacgggac gccgaacgac ggcaccagca atgtgagcct ggtcacggcc
     1441 aactgcgaca gcctgggcaa ccagtacgcc atcaagatct ggtggctcga agaccgctcg
     1501 gagaccaacc cgaccggtac gctcaagcgg ttcacgacgg cgatccggcc atgaaccagc
     1561 gcgcgcggca agcgggtttt accctgatcg aaatgatggt cgcaatggcg ctcggcctgc
     1621 tgctggtcgt cgtcatcggc caggtcttcg tcaccagcaa ggagtcctac cgcacgaccg
     1681 aggacctttc gcggctgcag gaaaacgcgc gcttctccgc cgcccagatg agccgggtga
     1741 tccgcatggc agcgtttgtc acggatacca acaacccggt ggccgcgcgc ggggtgatgt
     1801 ttccctcggc aaccgcgccg gcgctcacgg gggccgacgg ccttgccggc gccccggatc
     1861 aggtcacggt gcgctacgaa ggcagcggcg cgggcaccgc ggacggcacg gtcctcgatt
     1921 gccagggcaa tgccatccgc gcgggcgaca tggccgtggc gcgcttctac ctcgccgcgg
     1981 gcgccaacgg cgcgagcgcg ctcttctgcg acaacgccgg cacggtggga ccgggcattg
     2041 tcccggccgc ggtcgagctc gcatccgatg tcgagaacat gcagatcctc tacggcgaag
     2101 acaccgacgc agacggcacc gccaaccgct acgtcgagcg caccaccgtg cccgccctcg
     2161 actacgagag agtcgtgagc gtgcgcgtcg cgatcctcgt gcgctcgacc aaccaggttg
     2221 ccgcatcggt caacaccagc acctacaacc tgctcggcac ggtgttcgac cccgtcgacg
     2281 accgccgcct gcggcgcatg tacacctcga ccatcacgct tcgcaaccgc gcgccatgaa
     2341 acgcctaccc ctccgcaccc agcgcggcac cgcgctgatc atcgcgatgc tgttcctcgt
     2401 gatcctcggc atgctcggcg tcaccaccat gaccgccacc acgctcgagg aacgcatggc
     2461 gggcaacacg cgcgaccgcg acatcgcgat gcaggcggcc gaggccgcgc tgcgcgacgc
     2521 cgagcgcgac ctcaccaaca cggttgcggg cagcggccgg gtggtgacgc tggccatgtt
     2581 cagcgcgggc tgtaccggag ggctgtgcac cgaaggcgtg gcgattttca ccaacatcga
     2641 cgacgccgcg aagagcgcgt acttcacgca gtttgcgaac ccagcgcagg cgttgcaagg
     2701 gacggcgcag cagccgcgct acatcatcga gctgctgacc gccatgccgc cgcaggttcc
     2761 ggcgccgccg gtgggccagc agatccgcaa cttccgcatc acggccaaag ggttcggccg
     2821 aaacgcgaac acgatcgtca tgctgcagac cgtgttccag ctgaccatat gagcacatca
     2881 tgaagacttc gatccgcacc atcgctctgg cgctggccgc ttcggccgcc gcctccaccg
     2941 cgcacggcgc cgggctcacg ctcggcacca cgccgctcta cctcggctcg agcgtcgcgc
     3001 cgatcgtgat gctctcggtg tcgaaggacg agcagctgtt ccagaagctg tacaacgact
     3061 acaccgacct ggacggcgac ggcctgctcg acattacgta caagcactcg atcaactact
     3121 acggctactt cgacggcttc aagtgctaca actacgacac ggcgctcagc cgcttcgtgc
     3181 cgatcgcgct cagccccgcg agcaacaagt attgcgacac cgtggccgcc gggcgctgga
     3241 gcggcaactt cctcaactgg gcgaccatga cgcgcgccga ctcggtgcgc aagatcctct
     3301 acggcgggca tcgctcgacc gacaccgcgg cgctgacggt gctcgagcgg gcgaatatcc
     3361 ccttcgacac ccacgctttc gccaagtact accacggcgc cgacatcaac cggctgacgc
     3421 cgttcaccac gaccgtcact acgccgacga cctcggcggc gacgggcacc gacgctctgt
     3481 ataccggcgc cattgcgcag cccatcagcg ccggcacgaa acatttccga aacattggtg
     3541 tggccgtttg caagggagac caggtcaagg tcaaccttgg cgccgcgact accgtcaact
     3601 atgtggttgg ctatgtatcg gctgtgaacg gcgtcgggga ctgcagcacc accggaggca
     3661 ccagcggctt ctccatcgta gtcaagaccc aaggcgtgtc gggcgccggg gccgggtcca
     3721 atgcaggcgg tgcctacacc ctgaccaatc tcggctcgac cggcatcacg atctgcaacg
     3781 tcaccgacgc cggcagcacc aacctttcgc ataccacgac caacccgccg ctgatcaagg
     3841 tcgcgaaggg caacttcgcg atgtgggcga acaacgacat cacgcaatgc cagtggaacc
     3901 cggcgaacgg cacgcgcacc ttcggcggca ccggctcgaa tggcaacgaa ggctcgctca
     3961 gcggcggtct tccgacgctc tttccggccg gcagcgtgaa ctggtcgagc gcgcacgagc
     4021 cgatccaggc gacgcacggt ctcgccgtcg gcggcgtgtt caacttcgtc gcgcggatcg
     4081 aagcctgcaa ggcgggggtt ctgggcttcg agcgctgcaa ggcctacggc gccggcttca
     4141 agccgatcgg gctgctgcag ttctacggcg agtccgacct gctctacttc ggcctcatga
     4201 ccgggagcta tgcgaagaac aagtccggcg gcgtgctgcg caagaatgcc ggcacgttca
     4261 ccaacgaagt gaacccggcg aacgggacgt tcctggtgcc ggcgggcggc ggcatcgtcg
     4321 acacgctgaa ccggctgcgc atctacggct acgactacgc caccaccagc tatggcgggg
     4381 cggcgcccga ccgggatggc tgcggcgtcg tcacctccgg cagcggcacc gccgcgctga
     4441 accgcggcga cgcgcaggaa ggcctgtgca cgagctgggg caacccggtg gccgagacgt
     4501 atctcgagtc gctgcgctac ctcgcgggca agaccgtcga ccccaccttc acctacgccg
     4561 cgggctccaa ggacgaccag gtgggcggcg cgcccggctt gcccaagccg gcatgggtcg
     4621 accccatgtc cgacacgacc aagtactgcc gcccgctgaa cgtgctcgtg ttcagcgcca
     4681 acgtgacgag cttcgacaac gaccagctcg gcaacgtgac ccagctcaat accgcgagct
     4741 cggcggtcac gctcaccaac gcggtcggca ccgccgagac gatcaatggc aacaacttct
     4801 tcgtcggtaa cgacggcgcg ggaaacaaca acgagctttg cacgctgaag aacgcgatca
     4861 gtttcggcag cgtctacggc gtctgcccgg aatcgccgcg cggccgcggc agctacctga
     4921 tctcgggaat cgcgcaccac gcgcacacca accgcatccg cgtcgcgccc gacccgaccg
     4981 cgctcgcggt gccgccgacc aacacacgcg ccctcaaggt caacacctac ggcatcacgc
     5041 ttgccgcaaa cagcggcacg gtgcagatcc cggtcggaac caacaccgtc accctgctgc
     5101 ccatcgcacg agtgcaggac atcatcgtcg gcgccggctc gggcggcctg atcgacttca
     5161 aggtgatcca gcaggacgcc accagcggca agttcttcgt gcagtgggag gtgtcgcagc
     5221 agggcgcgga ccgcgacctc gacctgtggg gcgtcatcag ctacacgatc aacaccggcg
     5281 tcacgccgaa cacgatcacg gtcacgaccg acgtgatctc ggcgaacccc agcggcttcc
     5341 tcggcttcgg ctacgtgatc gccggcacca accaggacgg cgcgcacttc cactcgggct
     5401 acaacacgtt caactacacc tacaaccccg gcggcatcgt cgagtgcacc aactgccaga
     5461 ccgcggatgc gccgacctcc aagacgtaca acgtccagaa ctcgacggcg cagatcctga
     5521 aggatccgct gtactacgcc gccaaatacg gcggcttcaa cgacgtcaac gcgaacagcc
     5581 tgccggacca gacgatcgag tgggacagca agaagatcga cggcagcgac ggcaccgacg
     5641 gcattccgga caactacttc ccggtgacca acccggccgc gctggaggcg gcgctcgatc
     5701 gcgctttcat ctacatcctg caggtgtcgt cggcctcctc ggtggcgacg aactcgacct
     5761 cgctcaccac cggcagccgg gtgtaccagg cccgcttcaa cagcaatgaa tggagcggcc
     5821 agttgctctc gttcaccatc ctgccgaccg gcgtcatcga cccgacgccg aacttcgacg
     5881 ccgggcagct gttgccggcg tccaactcgc gcatcatcac gacctacagc cccagagccg
     5941 gggcgccggg ggtgtcgaat atccctgcgg gggtcccgtt ccgctggggc agcctccagg
     6001 cggcccagca gaccgcgctg cgcaaggacg gcagctcgca gatcgtcgac agcctcgtcg
     6061 gcggcgcgtg cacctcggac aacgttccgg tgggctgcac cgaccgcggc tcgctgcgcc
     6121 tggaatggct gcgcggcaat ccggtcgccg aaggcaccac ggcgacggcg ctgcgcgtgc
     6181 ggccgagcac caagctcgcc gatatcgtca actccacgcc gcgattcgtc gggccgccca
     6241 gcgcctcctt taccgatcca aactacctgg ccttccgcag cttctatgcc ggccgcacgc
     6301 cgatgctcta cgtcggcagc aacggcggca tgttccatgc cttcgaggcg acgaccggag
     6361 ccggtggcgg catcgaaagg ttcgcctacg tgcccggcaa gatgtatccc aacctcaccc
     6421 ggctgacgac caagccttat tcccaccgct acttcgtcga cggcacgcct tcagtcaccg
     6481 acgcctgctt cggcgcgtgc ggcgccatga acgactggaa gaccatcgcg gtcgggggct
     6541 acaactccgg cggacagggc tactacgcga tcaacgtcac cgatccgaat tcctggtcga
     6601 ccgaggccgg agcggctgct tccgtgctgt gggaattcac cgacgtcaac gatcccgatc
     6661 tcggcttctc gtacagcaaa ccgctgatcg taaagatgtc gaacggccgg tgggccgcgg
     6721 tgttcggcaa cgggtacaac aactcgatcg cggagagcgg cgagctcgcc tgcgacatcg
     6781 gcgacggctc tgcaggcaac ccgtacgggc gcgccgacgc ccagccgggc tgcagcacaa
     6841 gcctgagcgg ccatgcgtac ctctacatcc tgttcctgga cggcggcacc gacggcacat
     6901 ggacgcaggg cgtcgactac atcaagataa acaccaatac cggcaccgat actgcgacgc
     6961 cgtccaacct cacgccggga acgccgaacg gaatcgccac gccggtatcg gtcgacttca
     7021 acggcgacgg catggtggac tacatctacg ccggagacct ggtcggcaac ctgtggaagt
     7081 tcgacgttac cagctcgtcg ccgggaggct ggggcgtggc ttcggcgggg gcgccgctct
     7141 tcatcgcgca ggacgcgtcg ggcaaccgcc agccgatcac ctcggcgccg acgctgttcc
     7201 cgcacacgag cggcggcttc atggtgctgt tcggcaccgg caagtacatg gagatcaccg
     7261 acgacaccgg tccgttcaac atgagcgcct tctacggcat ctgggacaag ctcgacaaca
     7321 gcacggtgac gcgctcggag ctgatgcagc agaaggtgct caacgtgagt gcgggtaacc
     7381 tggacggcgg gctcgtcgtc ggcacgaaca aggtgcggct caccagcgcc tatgtgccga
     7441 actacagcgg tgcagaccgg accaataccg tgttcggcga cgcggacacc gacccggagt
     7501 cccaggcgcc gaccgcgatc acgcccgcca accagcgcgg ctggttcctg gaaatgccga
     7561 attccggcaa cggcggcccg ccgaacgccc cggccaccgg aaccggcgag aagatcgtgt
     7621 tcgacccgct catcagcacc gggaagatcg tgttcaccac gctgcttccg tcgacgcagc
     7681 cttgcgacgc cggcggcacg agcttcatca tggacatgga cccggtgtcg ggaagccggt
     7741 tgtcgttctc gcccttcgac ctgaacggcg acagcaactt cagcagcgcc gacttcgtca
     7801 gctacggcgg caagatcatc gcggtgtccg ggctcggctc gaccatcggc atcgtgccgc
     7861 agccgacggt gatccagggt ccggccggcg gcggcatgga gatcaaggtc ctgtcgggct
     7921 cctcgggcgg cctgcagacg gtcctcgaga acgcccccga gcccacgccg acccccggcg
     7981 cgcggacctc gcggcgcctc atctggcgcg agctgttctc caactagaca ataacgagaa
     8041 gcccaataac cgaggaccga catggctaac ctgcacgccc gccgcggacg cggctttacc
     8101 ctgatcgaag tgatgatcgt cgtggcgatc atcgggttcc tcgccgcgat cgcatacccc
     8161 gcgtatacca accacattcg caagggcaag cgggcggaag ccaaggcgag gcttctcctc
     8221 gtgtcgcagc ggctggagcg cgcctatacc gacaacggca cctacgtcac cgatgtcgct
     8281 cccttggtcg gcctgaccgc cggcgccacc gtgtactcgg ccgacaacaa cgatgcctac
     8341 agcggctatc gcatcacggt ggccccggcc gccggcggca tcaacaccgg ctataccatc
     8401 accgccacac taaatgccgg cttcaccgat gacgagtgcg gcaaccttac gttgacgagc
     8461 accggagtca agggaaagac cggctcggcc cccatcagcc gctgctggtg agccccgagc
     8521 ggcggctcgc cttttccgtg gccgcgtcga gcggcctgca cctcgggctg ctcgccggcg
     8581 cggcgagcct gctctcgccg ccgaacgacc atttcctgcg cgatgcgcat cgcggcccgt
     8641 cgctcgaggt caccctgatc gccgcggcgc cgcctgcgct ggtcatagac gcggcgctgc
     8701 cgccgatggc cgatgcggtg cgggtgcttt ccaagcgagc ggcgccgcgc gagaagccgg
     8761 gcgccgagcg cgaagcgccg cctgcgaagc cggagacggt gtactactcg gcgcgcgagg
     8821 tcgacgtgcg cgcggtcccc atcggagaca tcgtgcccat caatcccgac ctcagcggtc
     8881 gggaagccgg aatggtggtg ctgcgactgc tcatcaacgc cgcgggcaac gtcgacaacg
     8941 tgctcgtcct gaaagccgag cccgccagca gcttcggccc gcaagtcctg ctgcccttca
     9001 agcaggcgcg cttcctaccc gcgcgcaagg ccggcatcgc ggtgaacagc gagatgctga
     9061 tcgagctgcg ctacggcgcg ctcgaaacga ccaagcccta gcgcgcggcc cggacgagct
     9121 cgcgcggcag ggtgaacacc acgcgctcgg tgattccggg aagcacccgc acgttctgcg
     9181 ctccgagcgc cttgagccgc gcgagcagct cgtcgaccag cacctgcggc gccgaagcgc
     9241 cagccgtcac gccgacgcgg cgcttgcccg cgatccactc cgggcgcaag tcggcggcgc
     9301 ggtcgaccat gtaggcctgc gcgcccatgt gctgcgcgac ctcgcgcagc cggttcgagt
     9361 tcgagctgtt cggcgagccc accacgatca ccacgtcgca ctgcggcgcc atcagcttga
     9421 ccgcgtcctg ccggttctgc gtggcgtagc aaatgtcgtc cttcttcggg ccgcggatcg
     9481 ccgggaagcg cgctttcagc gcggcgacga tggctttcgc atcgtcgatc gacagcgtgg
     9541 tctgggtgac gtaggcgagc ttgtcgggcg tgccgacctg caggcccgcc acgtccgcga
     9601 cggtctccac caggtacatg ccgcgcaccg cctggcccat cgtgccctcg gtctcgggat
     9661 ggccgcgatg gccgatcatc acgatctggt agccggcctt cagcatgcgc gccacctcga
     9721 cgtgcacctt ggtgaccagc gggcaggtgg cgtcgaacac cttgagtccg cggcggtcgg
     9781 catcggcgcg cacctgctgc gacacgccgt gcgcgctgaa gatcaccgtg ccgcccggcg
     9841 ggacctcgtc cagctcgtcc acgaacaccg cgcccttggc gcgcaggtcg tcgaccacgt
     9901 acttgttgtg cacgatctcg tggcgcacgt agatcggcgc gccgtgcagc tcgagcgcgc
     9961 gctgaacggt ctcgatcgcg cgctcgacgc ccgcgcaaaa acccctggga ttcgccagca
    10021 ggacttccat cggcctattt tctcatgccg tacgcgacgt cgaatgccgc ttccaggcgt
    10081 gggtggctgc cgcgcagacc atcgaccacc cgcttcgccc agtcgaaata ctcgcggcgg
    10141 cgcgccaagt cccagcccgc cggcggccgc tcggcaacgt cgcgcaggtt gcagatcttg
    10201 tcggcgagct tgaccagctt cgcttccggc gacaggcgcg cggcgttctc tacctgcagg
    10261 cgcttgcggt cagccttgtt gagcgccttg tcatcggtga cctcggcgac gatgcgcgcc
    10321 acgcgcgcgc cgaaggtgtc aaccagctcc tcgggcgtgg tcgccgtatc ctccaccgtg
    10381 tcgtggagca gcgccgcaca cagcacttcc acgtcgctca cgcccccttc gttcaccagc
    10441 acgtccgcca gcgcgatcgg atggttgatg tagggcgatg cttcgtgatc cttgcggcgc
    10501 tggtcgcggt gcttgtgcgc agcgaaggcg agcgccttaa gaaggagcgc cagctcgctt
    10561 ttcatgatgc acgaacccgt cgaggatcag gagcaccgcg ccgacggtaa tcgccgaatc
    10621 cgcgacgttg aacgccggcc agtgcggcca gcgctcgagg atgtaaaagt cgagatagtc
    10681 gaccacgcag ccgaagcgcg cccggtcgat cacgttcccc agcgcgccgc cgacgatcag
    10741 gccgagggcc gcgcagaaca gcctctttcc cgggttgcgc acgatcagcg tgccgcaaag
    10801 caccgccgcg gcgagcgcga acacgatgaa cagcggcgtt tgccaccccg gggcgtcggc
    10861 cagaaagctg aacgccgcgc ccttgttgca caccagcacc aggttgaaga aaccggtgat
    10921 tgcctctcgc ggcgcgcgat cggcgaaata gccgagcacc gcccatttcg cgagctggtc
    10981 agcgacgacg atcgcgccgg ccagcgcgaa ccagcgccaa gcgctagaca tacttgcgct
    11041 tctcgcccgg accgcgcagg ttggtttggc agcgggcgca caggccctcg ccgttcacgt
    11101 ccgcggtgta gtgccagcag cgctcgcatt tggggttgcc gctcggcacc acgttggcgg
    11161 cgagcgcgtc cccgcgcgcc acgcgcgccg cggaagtcat catcaggaac ttgagctcgc
    11221 ccgcgaggct ccctagcagg tcgtagtcgg aggacggcac cgtcaggtcg acctcggcct
    11281 gcagagacga gcccaccttg cccgcggcgc gcaggtcctc gatctgcttt cgcaccgggg
    11341 tgcgcagctc gcgcaggcgg ccccatttgg cgaggagcgc ggcgcggtcg acaggagccg
    11401 gcacctcgtg ccaggtgtgg aagaagacgc tgtcgtcctt tttcccggga aagagctgcc
    11461 acagctcctc ggcggtgaag gacaagatcg gcgccatcag gcgcaccagg ctgtgggtga
    11521 cgtgccagag cgcggtctga gccgagcgcc gcgcagccga atcggcgcga caggtataga
    11581 gccggtcctt gagcacatcg aggtagaagg cgccgagatc ctcggagcag aaggcctgca
    11641 gcttctgcgc caccaggtgg aactggtagc gtgtgtagtc ggcagtgacc tcgtcttgca
    11701 tcgccgcagt catggcgagc gcgtagcggt caatctcgac catgtcggcg ggggcgacgg
    11761 cgtgcttcgc gggatcgaag tccgaagtgt tagcgagcag aaagcgcagc gtattgcgaa
    11821 cgcgccggta gctctcgacc acgcgcttga ggatctcgct cgagatcgtc agctcgccgg
    11881 aatagtcggc gctcgccacc cacaagcgca ggatttccgc gcccagggtg ctcgcgatct
    11941 cctgcggcgc catggtgttg cccttcgact tcgacatctt gcggccttcc atgtcgatga
    12001 cgaagccgtg ggtgaggagt gacttgtacg gcggcgcgcc gtcggtcatg caggagatca
    12061 gcagcgacga atggaaccag ccgcggtgct gatcggagcc ctccaggtac atctcggccg
    12121 gataggtcga ctggctgcgg tgcgagccgc gcagcacggt ctggaacgtg gagcccgagt
    12181 cgaaccagac gtccagcgtg tcccggctct tctcatactg agcactctcg gaaccgagaa
    12241 gctcctgcgc cgtaacgctc tgccaggcct cgatgccgcc ctgctccaca cgctgcgcca
    12301 ccttctcgag cagttcgggc gtgcgcgggt gcagcgcccc ggtctcgcgg tgcaggaaga
    12361 agggcatcgg cacgccccac tggcgctggc gcgacagcgt ccagtcgggc cggttggcga
    12421 tcatgccgtg aagccgcgcc tggccccacg ccgggaagaa ctgggtgttc tcgatcccgc
    12481 gcagcgccgt cgcgcgcaat gattctccct tcacgggctt gtccatcgag gcgaaccact
    12541 gcgtggtggc gcgcaggatc accggcgtct tgtggcgcca gcaatgcatg tagctgtggg
    12601 tgtgcttcca gtccaccgcg aagagcgcgc ctttctcctc gatggtcttg acgatcttcg
    12661 gattcgcatc ccagatcgag agaccgccga agagcggcag cgatgcggcg aacttgccgt
    12721 cgcccatcac cggcgtgagg atctcgtcgt ccttcatgcc gtagcgccgg cacgactcga
    12781 aatcctcgac gccgtacgcc ggggccgagt gcacgatgcc ggtgccggcg tcgagcgaga
    12841 cgtactcgcc gaggtagacc ggcgcctcgc gctcgtagaa cgggtgcctg aagcggatct
    12901 tctcgagggc tttgcctttg caggtcccca cggtcttgcc gctcaggccg tatcgcttga
    12961 ggcacgcctc gcgcagttcg ctggcgagga taagcaggcc tttgtccgtg tcgaccaggt
    13021 cgtagctgag ttccggatgg acgttgagcg cctggttgcc gggcagcgtc cacggcgtgg
    13081 tggtccagat tacggcgaag ccgggtttgg tcaccttggt gctgaagacc tttgcgacct
    13141 tctccggttc ggcgaaagcg aaccccacgt cgatcgccgg atccttgcga tcctcgtact
    13201 cgacctcggc ctcggccagc gccgagccgc agtcgaagca ccagttcacc ggtttcagtc
    13261 cgcggtagac gtagccgcgc tcgagcaacc ggccgagcac gcggatttcc tcggcctcgt
    13321 tgcggtacgc catcgtcagg tacgggtcgt cccactcgcc gagcacgccg aggcgctgaa
    13381 actgcgtgcg ctgccgatcg atctgctcgg tcgcgtaggc gcggcacagg cgcagcgtct
    13441 cgtcggtggg caggttcttg ccgtgcttct tctcgatctg cacctcgatc ggcatgccgt
    13501 ggcagtccca gccgggcacg tagggcgcgt cgaaccccgc cagggtcttc gacttgacca
    13561 ccatgtcctt caggatcttg ttgaccgcgg tgccgatgtg gatgtcgccg ttcgcgtagg
    13621 gcgggccgtc gtgcagcacg aagcgcggcc gccccttggc gagcgcccgc aggcgccggt
    13681 acacgccctt gtcgtgccac tccttgaccc acacgggctc tcgcttggcg aggtcgccgc
    13741 gcatcggaaa cggcgtttcc ggaagattca gcgtgtcctt gtaatcagcc atgttcgagg
    13801 aagtattcct ttgcctggcg ggcgtcacgg gcaatcgcgg cctgcagcgc cggcaagccg
    13861 tcgtacttct cctcgtcgcg cagcttcttc aggaagcgca cgcgcaggtg ctcgccgtag
    13921 aaatcgccgt aatggtcgaa gagatgcacc tcgagcagcg gaattgccac cgggttcacc
    13981 gtcgggcgcc ggccgacgct cgccacgccg cgcccgtagc gatcgacctc gaccacgaag
    14041 atccccgcga tcggcggcgg ccgccgcagc acgatgttcg ccgtcggaaa gccgaggttc
    14101 ttgccgagct tggcgccgtg cgcaacgcgg ccgctgatgg tgtacgggtg cccgagcagg
    14161 cgctcgccct cggcgaggtt gccggccgcg agcgctgcgc gcaccgcgga gctcgaaacg
    14221 cgcttgccgt cgaacaggac gtcgggcatc gtctcgaggg caaagccctg gttctgcagc
    14281 gtggcaaagc tcccggcgcg acgagcgccg aagcggaagt cgcgccccac cagcagccat
    14341 tgcaccgcca ggccgcgcac caggacctcc tcgatgaagc gctccgcggg cagcgcggcg
    14401 aacttcgcgt cgaagcgcgc cacgtggacg cggtcgacac ccgcctgcgc catgagctcg
    14461 agcttgtcgc gcaaccgggt aaggcgcgcc ggcgccgcgt cggcggcgaa gaattccctg
    14521 gggtgcggct caaaggtcag gatgcaggag gtgagcttcg cttcgcgcgc cctggcggtc
    14581 acccggtcga tgagcgcgcg gtgaccccgg tgcacgccgt cgaagttgcc gatcgtcagc
    14641 gcacaccggc cccgggcgcg ctgcaaagag ccgtgcgtga cgaccatgga ctcggttaac
    14701 acgctgaatt atagtgtctt tatgaaaaat atcgaagagc agatgagtat ctatgcggcc
    14761 taccaccaag acgcgcgcaa caaggtcacg cacttcatcg gcgtcccggc gatcatcctg
    14821 tcgctcttca tcccgctcgc ctggatccgc ttcgatgccg gcggcgtgac catcaccgcg
    14881 gcgatgctgt tcgccgccgt cgtcgtgctc tactacttct tcctcgacgt tgcgcttgcg
    14941 ctggccatgc tcgcggtgac aggggcgctg gtctggctcg gccaccagat agcgcaactc
    15001 ggcacagcac aaggctggat ctggttcgcg ctgctttttg tcggcggctg gatcctgcag
    15061 ctcgtcggcc atgtcttcga aggccgcaag ccggcgctcg cggacaacct gttccagatc
    15121 ttcgtcgcgc cgatcttcct cgccgccgaa gtgttcttcg ccctgggcta caaaccgcag
    15181 ctacatcgca ggatccagga acgagctcag ttcagccgag cgcaaagcgc ggacagctcg
    15241 aacaacttgc cgcgcacctc gtccaggtcg ggtgcgtcgg gctcgcgctc ggcgtagtcg
    15301 gtcaggtcct tgagcgcggc ccggtagcac tccaggcgct ggtagacgta gccgcggtcg
    15361 cgcagctcgg cgctcgcgtc gggcgtgacg agcagcatgc ggttcagcac gtcgagcatg
    15421 cgctcgggct tgtcgcgctc gcgatagatc cccttcaggt tgcgcagcaa gcgcgacagg
    15481 atctgccggt tggtcgccgg ctcgaggaac tgatcgagcg gcaactctgc agccggcaga
    15541 tcgtcggcga cgccatcggg gatcacgcgc tttacgcggc tgcgcagctc ggcctccgac
    15601 tgcggcgcgc cgcccgcgaa cgggtcgagc accagcgtcc cgccccgcag gcgcaggcgc
    15661 acgagaaagt gcccggggaa cgacacgcct tcgagcggca ggccgatgcg ccggccgagc
    15721 tccatgtaca gaatggacat cgtgatcgga atgccggttt tccgctcgat gacctcgttc
    15781 aggtagctgt tgcgcggatc gtagtagtcg tcggtgttcc cccagtagcc gaggtcctcg
    15841 tagaggaact ggttgagcgc gaccacgcgc tcctcggcgc cgccggtctt cggcaggcgc
    15901 ccgcgcaggc ggattgccat gcgctcgatc tcgcccatat agcggtcgac gtcgagcccg
    15961 gggtaggcgt cctcggcgat catcaggcac gcccgcgcga ggtcgatgcg cgcgtcgtcg
    16021 cgtgcgatga gcgcggagaa ggcctcgagc gcgacgccca tcactccgcc gcgcgccgcg
    16081 agaaatcgcg cggccggaag ccgaaggcga agaggcaccc ggcataggcg gcggtgccga
    16141 ggagcaccag gccgaggatc gcgggcactt tgagctgcca gcccgcctgg agccaccacc
    16201 gggcctcgcc catcgtcgta aagagcacga tcgccatgaa tgccaccgag gcgaggatct
    16261 tgagaatgaa gaccggccag ccgggctgcg gcaggtagat ttttgccctc ttcaacatga
    16321 agtacaagag caatgcgttg aggcacgctc cgaggccgat ggcgagcgca aggccggcat
    16381 ggcgcagggg accgaccaga aacaggttca tgagctgcgt ggcgaccagc gtgaagatgc
    16441 ctatctttac cggagtcgcc acgtcctgtc ttgcataaaa gcccggcgcg aggatcttga
    16501 cgaggatcat ccccaccagg ccgaggctat aggcgacgag cgcctggcgc gtcatccagg
    16561 catcctcggc gctgaagcgg ccgtagtgga acagcatcgc gatgagcggc agcgcgagca
    16621 cggcgagcgc tacggcgctt ggcacggcga gcagcacggt gatacgcagg ccccagtcga
    16681 ggagcttgct gtactccgtg gtgttggccg cggcgtggta cttcgacagg ctggggagaa
    16741 ggatcgtgcc gaccgcgacg ccgagcaccc ccgcgggcag ctccatcagt cggtcggcgt
    16801 agtagagcca cgacacgctg ccggtaggca gaaaggacgc gaagatctgg ttgagcagca
    16861 gcgaaacctg gctcaccgag accccgaacg ccgccggcgc catcagcagc aggacgcgcc
    16921 gcagcccggg atgcgaaaaa tccagtcgcc agcgcggcag cagcttcatt ttcatcagaa
    16981 aagggatctg gaaagcgagc tgcaggagtc ctcccgcaaa caccgcccag gcgagcacca
    17041 tcaccggcgg atcgaaatac tccgccagga acgcggcgcc gacgataaag gcgacgttga
    17101 gcagcgccgg ggtgatcgcc ggcaccgaga aacggttcca ggtgttgagg acgccggcgg
    17161 cgagcgccac cagcgagatg aacacgatgt acggaaaggt gatccggagc agccccacgg
    17221 tcagcgagaa tttctccggc tccgcggcga accccggcgc cgtcaggtag acgatgagcg
    17281 gcgccgccgc catgccgagc gccgcggcga gcaccagggc aaggaacagg accgtggtga
    17341 tcgagtcgat cagcgacttc gtctcctcgg gggaggtgcg gttcttgtac tcggccagga
    17401 tcggcacgaa cgcctgcgag aacgccccct ccgcgaagag gcgccgcagc aggttgggaa
    17461 tcttgaaggc gacgaagaac gcgtcggtgg ccaggcctgc gccgaacacc cgcgcgatga
    17521 agaagtcgcg cacgtagccc aggacccggc tcaccatcgt caggctgctg accgtcgcca
    17581 ggatccgcag gaggttcatg ctaaaatttt ataaatggca aacatcaaat ccgcccggaa
    17641 gcgggccaag caggcggtcg agcgccgcgt gcacaacatg agcctgcgca ccgaggtgcg
    17701 cacggcgatg aagaacgtga agaaggccgt cgccgccggc aacaaggaca ccgccgcgaa
    17761 ggcgctgcgc gagtcgcagc gcgtcatcga ccgcatcgtg gcaaagggtg tcctgcaccg
    17821 caatgccggc gaccgtcaca agagccgcct ggcacacgcc ctcaagggca tgaagtagac
    17881 cgcggttacc acgcagttac cactcagtta cccactacgc cgtatccccg acacgctgcc
    17941 actcggcgag cctggtccgc tccgcgacgc gcccgtcgcg cacccgcagc tcgttgtcct
    18001 tcgcgaagtt gacgagaaag cggtaggcca tcggctcgat ctgctcgagc cgcacgtcga
    18061 ccaccacgca gcgcacgccg ttcgcggtga tcggatggac gaacggcgag tactgcatgc
    18121 gatggtcgcc gccgcgacgg tcgccgccga aggctgccat caccccgcac agccgctccg
    18181 cccagtcgct cggccggaaa ggtcttccgt cgagcgtgat tccctggatg acgaactcga
    18241 cgaccgcctc ttctgccatg gccaattata gtaagaaggc ctgatgacca agccttcgag
    18301 ccggatcctc gtaatcaacg acgagcatct cgtgctgcgg gaattcgtca agggcctgaa
    18361 cgccgcggcg cgcacgctcg acaaccccct cggcatcagc ttcgccggcg tcaacaccgc
    18421 gaaggaagcg ctgcgcgtga tcgaggccga cggcaacctc caggccgtgc tcgtcgacga
    18481 cacgctctac accttggggt cgaattcggg ctcccattcg aagaagaagc tgcagatgtc
    18541 ggcgctcgag ctcgtgcagc gcatcacgcg ctttcgtccc gagctcgacg tctacatcgt
    18601 catcgcgcag gaaaaggagg acgacgtcgt cgacgcgctg ttcgccgagt cggtcgacgg
    18661 ctacttctac cgcgaggagc gcgactaccg cggcatctac cgcatcctga acgcgcagat
    18721 ccaggagcgc gcgcgcacgc cgttctacga ccagctcaag aactacgtct ggatggcgaa
    18781 ggaccagtgg cataccccgg gccattcctc gggcgaatcg ctgcgcggca gcccctgggt
    18841 caacgatttc tacgaattca tgggcgagca cgtgttcgac gccgacctct cggtgtcggt
    18901 gccgatgctc gattcgctga tggagccgac gggcgtgatc ggcgaagcgc aggcgatggc
    18961 ggcgaaggcg ttcggcgcgc gccgcacctt cttcgccacc aacggcacat ccaccgcgaa
    19021 caaggtcatc ttccagaccc tgctcgcgcc gggcgagaag ctgctgctcg accgcaactg
    19081 ccacaagagc gtgcaccacg gcgtggtgct ctcgggcgcg catccggtct atctcgactc
    19141 ggcgctgaac cgcaagtacg gcctgtatgg ccccgtcccg aaaaaaactc ttttgcgaga
    19201 aataaaaaaa catcctgacg cgcaggcgct catcctcact tcctgcacgt acgacgggct
    19261 gcgctacgac ctcgcgccga tcgtcgaggc ggcgcatgcg aagggcatca aggtgatcat
    19321 cgacgaggcg tggtacggct tcgcacgctt ccacccggcg ttccgcccga ccgcgctcga
    19381 ggccggcgcc gactacgcca cccagtcgac gcacaaggtg ctctcggcgt tctcgcaggc
    19441 ctcgatgatc cacgtcaacg accccggctt ccgcgagcac ctgttccgcg agaacttcaa
    19501 catgcacacc tcgaccagcc cgcagtacgg gctgatcgcc agcctcgacg tcgcgcgcaa
    19561 gcaggcggtg atggagggct acaagctcct cggccgcact ctggagctcg ccgccgaagt
    19621 caagaaactc atcaattcca ccaaggtctt ccgcgtgctc gagctcgacg acctcctacc
    19681 cgacgcggtg aagcacgacg gtatccggct cgatccgacc aaggtcacga tcgacatctc
    19741 gggatgcggc tacacggtgg aagacctgca gaaggcgctg ttcgagcgct tcaacatcca
    19801 ggtggagaaa tccaccttca ataccttgac gttgttattg acgataggaa caacccgatc
    19861 caaggtctcg cgcctctacg acgcccttat gcgcatcgcg cgcgagcagc gcgcgccgcg
    19921 gcgcctggtg cagacgcccg agatcccgcc gttcacgaag ctggtgttcc t
//
LOCUS       DATBSZ010000178        12341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1190201, whole genome shotgun sequence.
ACCESSION   DATBSZ010000178 DATBSZ010000000
VERSION     DATBSZ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12341
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190201"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..1686)
                     /locus_tag="VIG70_11545"
     CDS             complement(220..1686)
                     /locus_tag="VIG70_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8251234.1"
                     /translation="MSTITTDIPARLDRLPWSAWHWRVVIALGVSWLLDGLEVTVVGS
                     LGPALQRPDTLGLSASQIGWAASAYIGGAVLGALYFGRLADKLGRKRMFLVTLVVYLV
                     ATVLTAFSFDIFSFAFCRFLTGFGIGGEYAAINSAIDELIPARLRGRVDLAINGTFWI
                     GAAMGAGLSIVLLDPDFIGAEWGWRIAFGLGGILAFAILLVRRHVPESPRWLLMHGRA
                     EEAEAVVRRIEDEVGVAHPVNERLALRPLRSVSWSEVLHVLLVRYRSRAIFGLTLMAS
                     QAFFYNAIFFTYALVLARFYGVPDERVGYYIFPFALGNFLGPLLLGHLFDTVGRRIMI
                     ATTYAIAGMGLLVTGYAFSQEWLTPLTQALSWSLIFFVASAAASSAYLTVSEIFPLEM
                     RAISISLFYAAGTALGGFLGPPLYGAMIESGSRSALFGAYAFAAVLMCVAAVVTLFLG
                     VDAERRPLEQVCSPLGCDPDPLDLPAHPTRVENQRVNP"
     gene            1741..2694
                     /locus_tag="VIG70_11550"
     CDS             1741..2694
                     /locus_tag="VIG70_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8251235.1"
                     /translation="MKALFLLLGILLAGTANAQYPAKPLRLIVPFAPGGSTDIFARLI
                     AERAQAPLGQPVVVENRAGAAGNIGAEAVVRSAPDGYTLLMATTGVMAINNALYKSMT
                     YDAAKDLEPVVYVASITNVLIVPPDSPAKSVAELIALAKKEPGKLTFASSGAGASTHM
                     SAELFKSMAGIDILHVPYKGSGPAMPDLMSGRVSMMFENAPGAVSHIKAGKLRALAQT
                     GLKRSSALPEVPTVAESGVPGYESLSWSGIAVPAGTPRAVIDRLNKDLNSILAMADMR
                     QKLAEQGADTIGGPPEAFAAHIRAEREKWSRVIKTANIVVN"
     gene            2694..3893
                     /locus_tag="VIG70_11555"
     CDS             2694..3893
                     /locus_tag="VIG70_11555"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY8251236.1"
                     /translation="MGFLSARADRARLSASARAAQRARELRAAGKSIIELAQGEPDFN
                     TPDHVIEAAHRAMHAGQTHYTQVDGTPDLKAAIAAKFRRENGIECKPENVSAGAGGKQ
                     VLYNALMATLDPGDEVLIPAPSWVAYADITHFAEGVPVWLPTRPADGFKLSPQDLEKS
                     ITRRSKWLMLNSPSNPTGAVYTADELRALAAVLERHASLWIISDDMYEHVLFDGRPFA
                     TLAAVAPQLAARTLTVNGLSKTFAMTGWRLGYACGPVELIRAMARMQGQSSLNPSSVS
                     QAAGVAALNGPMDIVKSRCAEFQARRDFIVPRLNAIRGLACEAPHGAFYVYVSCAGWI
                     GKRTPQGNAIANDADVTAHLLENGVALPNGAGYGLSPYFRVSFASSLANLEEACKRID
                     AAARKLA"
     gene            3893..5131
                     /locus_tag="VIG70_11560"
     CDS             3893..5131
                     /locus_tag="VIG70_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY8251237.1"
                     /translation="MHLVERFADWAVSFRETPLEKTVLHHARRAVIDWHAALYPGMVV
                     PPVTLMKKAFAGESESPRLKALIEGTAAHTVEVDDIFRDGIYHPGAPTIAAAFAINQN
                     LVRAIVVGYEISTRIGAAMGKAHYKHWHNTGTIGCFGAAAAAAEALQLNRSQFAHALA
                     TVTTFAAGLQQAFRMDSMSKPLHAGRAAEAGVAAALMAREGITGSLDVMQGFAAAMGD
                     KPDFERGLATLGKDFHITRMTFKNHACCGHTFAAIDGALELKKRMQVKPEDIEQVEVA
                     TYKAGLEVAHYEAPRTPAEGRFSLKYVVATALSHGSVRLAAFEPQRLEDPVTRKLLQR
                     ISVSVDPELEAAFPAQRAARVAIRARGKREEWLQPTRIGDPDAPLSDRALEQKYFELA
                     TPVLGQEKAKEVLKRLWQLE"
     gene            5262..7190
                     /locus_tag="VIG70_11565"
     CDS             5262..7190
                     /locus_tag="VIG70_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY8251238.1"
                     /translation="MIDLAQALFSPRAVALVGASGDAAKTTARPQRYLKKHGYQGRVF
                     PINPTRNEVLGQRSYARVSEVPEPVDHAYILTGEVEQALEDCGKRGVAVASIFSGGFA
                     DAGPEGAKREAKLVARARELGVRLLGPNSMGVIDVPGRLALSVNAVLEMDALPPGTTS
                     IVSQSGTMLGTLLSRGAARGLGFGRLVSVGNEADVGVGELVDLLAADAGTRVILLFLE
                     TIRDAQRLASAVRAAHLAGKAVAAYKLGRSALGEAAALSHTGALAGTDAALDAYFRDC
                     GIVRVDMLETLLEIPPLLAGRVPLDLGRAGKVTVVTTTGGGAASVVDRLGMRGVELAA
                     PIMDLTMAASGQDYGAALAKAAQTNCDAVLACVGSSAQFHPDLAVQPIVQGPRVKPLI
                     AFFTPQADRSLALAAAAGIPAFRTPEACADAVASFLSWRSPRKPPALAVPADLPENPF
                     ELLAALGIPVARWAIAEAPAFAHGIEYPVALKRLEAHKTERGGVVLGIRDRAELEKQA
                     KKMNADRVLVQTMQSGLAEAIVGYRDDAVVGPVVLLGAGGVLTEIHRDYVLRMAPVSE
                     REAGEMIEQVKGFAAIRGYRNLPRGDLGALARAVSALSALALVPGRPLREAEVNPLIV
                     SAEGVVAVDALAARKGAA"
     gene            7187..8047
                     /locus_tag="VIG70_11570"
     CDS             7187..8047
                     /locus_tag="VIG70_11570"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8251239.1"
                     /translation="MIGFIGLGVMGEPICRNLAAKSGEKVLAYDVDPAPLGRLKEAGV
                     ETAASVREIAERCNFIFLSLPGVQQVKTVCVGPGSLMLYMIAGSYIIDLSTVTVALTR
                     DLQSRFSARGVYFADAPVARTREAAKKGTLSVMVGGTEQVYKRIRPLLVHIATDITHC
                     GGPGAGQATKLINNMVLFQNVVALAEAMTLARRAGLSATRVFEALMLGSADSFALRHH
                     GLNAMLGDEYPERAYSVEYALKDVQYLLEFARQARVELKGAKLAMEALERAAAAGNGA
                     KYFPVLVKTL"
     gene            8047..9414
                     /locus_tag="VIG70_11575"
     CDS             8047..9414
                     /locus_tag="VIG70_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8251240.1"
                     /translation="MPQAALREILLAAGLDFSEVDFAGDDPVLPIRYRVAAAGAASLG
                     ALGLAVAKLANRAQRISVNARAAAISLRSATYLRINGKPLPPVWDPLSGFYPVRDGWV
                     SIHCNFPNHREAAMGVLGAGADRARAEAASRAWDGEALEDAIHAAGGCAGFVRSQDRW
                     NAHAQARAVAQQPLLQIERIGDTPPEFSKNARPLGGVRVLDLTRVLAGPTCAKSLAEH
                     GADVLKISAAHLADSGLVELDTGMGKRSARLDLRNPGEVKAFQRLIREADVFVQSYRP
                     GALAGRGFSPDALAELRPGIVCASLSAWGPAGPWRERRGFDSIVQAVSGIAQAAGGDA
                     GSGDKPRLLPVSAIDYVSGYLMAFGVCVALARRATEGGSWLVRVALARVGKWIVDRGT
                     LEDYRGLPADLPESELAALFGEMDAPDGRIRYLKPVLGLSETPPYFARPPVPLGYHPP
                     VWTSS"
     gene            9399..10544
                     /locus_tag="VIG70_11580"
     CDS             9399..10544
                     /locus_tag="VIG70_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY8251241.1"
                     /translation="MDFQLTPEQAQFRDSVLAFSKKHLAAGARERAHKPDYPWDVARL
                     MAKQDLLGITLPEADGGQGGSLMDAVIAIEAVASVCPRSADVVQAGNFGPIRVLGEYG
                     SQFQKTRFLKKLLSGEAVISVGMTEPEAGSAVTDLQTSATPDGDGFRINGTKVFTTHS
                     AYAEVILTYVRFGPGVSGIGSVLIEKSAPGVRRGKPSTFMSGEEWAELHFDDVRVPGE
                     MVLLREGGFKKQMAGFNVERLGNASRSLALGRYAFERAREWALQRKQFGKLLAEFQGL
                     QWKFADMKIQLDAAQLLLYRAAANADRGMPSAEETAIAKAHCNRTGFDVANEALQVMG
                     ALGYSQEELVEYCMRRCRGWMIAGGSIEILKNRIAEGIFGRSFSQRG"
     gene            10541..10990
                     /locus_tag="VIG70_11585"
     CDS             10541..10990
                     /locus_tag="VIG70_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HEY8251242.1"
                     /translation="MINAARFLGLLFTALALAPALAHLLELPNKMGLSRDEYLVVQQI
                     YRGWSMLGVLVFGALLSTLLLTILVRKRPGEFGAALTAFLAIAGTQVVFWIFTFPMNQ
                     RTANWTILPDHWGALRMQWEYSHAAAAVLNLVALVAVIVSVLARRKP"
     gene            10987..11340
                     /locus_tag="VIG70_11590"
     CDS             10987..11340
                     /locus_tag="VIG70_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251243.1"
                     /translation="MIEDLAQLVNRDPAIVRWGRRMHETFMVEVGDTQYLLTVRDGRL
                     EVEKGPFTMRSWKFAIRASRECWEKFWQKTPAPGWHDLFALLRRGDVKFEGDQRVLMA
                     YLLYVKLVLAAPRNA"
     gene            11447..12274
                     /locus_tag="VIG70_11595"
     CDS             11447..12274
                     /locus_tag="VIG70_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8251244.1"
                     /translation="MKLEPIVGRYVRLNDCRIYFEEAGQGIPLLCLHTAGSDGRQYRG
                     VLNDAQITKSYRVIAFDMPWHGKSSPPAGWQNEDYRLTTGSYTAMIMDFIKTLGLERP
                     VVMGCSIGGRIVLDLALKHARELRALVGLQTGAFVERYYDAAWLDHPEVHGGRACGAV
                     AYGLMSPLSPESERWETVWHYMQGGPGIFTGDLYFYQVEGDIRGRIGAIDTSVCPLYL
                     LTGEYDYSASPRDTEAAAARIKGAKMTVMKGLGHFPMSENPEAFLGYLRPVLEEIRK"
BASE COUNT         2068 a         4085 c         4285 g         1903 t
ORIGIN      
        1 ggtggattgc tgcgtagtac gaatcaccgc gaaggaagag ctcaatcgcg cgttccagca
       61 gttcaagcgc gacgacgtgt ttctcggcaa ccgctgcggg cattcagtga ggcctaacgt
      121 ccaatagcca gaatatctgc cgtatatcac gattgccgca ttggagtatc agccgagaaa
      181 caatggcttg cgccgagcga ggacgtgata gccggcgcgc tacgggttaa ctcgttgatt
      241 ttcgacgcgc gtgggatggg cgggtaagtc gagggggtcg ggatcgcagc cgagcggcga
      301 gcagacttgc tcgagcgggc ggcgctctgc gtcgacgccg aggaagagcg tgacgaccgc
      361 ggcgacgcac atcaggacgg cggcgaaggc ataggcgccg aagagggcgc tgcggctgcc
      421 gctctcgatc atcgcgccgt agagcggcgg gccgaggaag ccgcccagcg ccgtgcccgc
      481 tgcgtagaag agcgagatcg agatcgcccg catctcgagc gggaagattt cgctcaccgt
      541 caggtaggcg gagcttgccg cggcggaagc gacgaagaaa atgagggacc agctcagcgc
      601 ctgcgtcagg ggggtgagcc attcctggct gaaggcgtag ccggtgacga gcaggcccat
      661 gccggcgatc gcgtacgtgg tggcgatcat gatgcgccgg ccgacggtgt cgaagagatg
      721 cccgaggagc agcgggccga ggaagttgcc gagcgcgaac ggaaagatgt agtagccgac
      781 gcgctcgtcg gggacgccgt agaagcgcgc gaggaccagc gcataggtga agaagatggc
      841 gttgtagaag aaagcctgcg acgccatcag cgtgagcccg aaaatcgcgc gcgaacggta
      901 gcggaccagc aggacatgca gcacttcgct ccaggacacc gagcgcagcg gccgcagcgc
      961 aagcctctca ttcacggggt gagcgacccc cacttcatcc tcgatccggc gaaccaccgc
     1021 ctcggcttcc tcggcgcggc cgtgcatcag gagccagcgc gggctctcgg ggacgtggcg
     1081 gcgcaccagg agaatggcga aggccaggat gccgccgagg ccgaaggcga tgcgccagcc
     1141 ccactcggcg ccgatgaaat cggggtccag gaggacgatg ctcaggcccg cgcccatcgc
     1201 ggcgccgatc cagaacgtgc cgttgatcgc gaggtcgacc cggccgcgca ggcgcgccgg
     1261 gatcagctcg tcgatcgccg agttgatcgc ggcgtactcg ccgccgatgc cgaagccggt
     1321 caggaagcgg cagaacgcaa aggaaaaaat atcgaatgaa aaggcggtaa ggaccgtggc
     1381 gacgaggtac acgaccagcg tgacgaggaa catccgcttg cggccgagct tgtcggcgag
     1441 gcgcccgaag tagagcgcgc cgagcacggc gccgccgatg tacgccgacg ccgcccagcc
     1501 gatctggctc gcgctgagcc cgagggtgtc gggccgctgc agagccgggc cgagagagcc
     1561 gaccacggtc acctccagcc cgtcgagcag ccagctcacg ccgagcgcga tcaccacgcg
     1621 ccagtgccag gcggaccatg gcaggcggtc gaggcgcgcc gggatgtcgg tggtgatcgt
     1681 ggacatgcgt cacctacgac acgctgtgcg gcggtgcgtt cgaaaggtaa catccgcggt
     1741 atgaaagctt tgttcctctt gctaggaatt cttctggctg ggactgcaaa tgcgcagtac
     1801 ccggcaaagc cgctgcgcct gatcgtgccg ttcgcccccg gcggctcgac cgacatcttc
     1861 gcgcgcctga tcgccgagcg cgcgcaggcg ccgctcggcc agccggtggt ggtcgagaac
     1921 cgcgccggcg cggcgggcaa catcggcgcc gaggccgtag tgcgctcggc gccggacggc
     1981 tacacgctgc tgatggcgac gaccggcgtg atggcaatca acaacgcgct ctacaagagc
     2041 atgacgtacg acgcggcgaa ggacctcgag ccggtcgttt acgtcgcctc gatcaccaac
     2101 gtgctgatcg tgccgcccga ctcgccggcg aagagcgtgg ccgagctgat cgcgctggcg
     2161 aagaaggagc cgggcaagct caccttcgcg tcctcgggcg cgggggcctc gacccacatg
     2221 tcggccgagc ttttcaagag catggccggc atcgacatcc tgcatgttcc ttataagggc
     2281 agcgggccgg cgatgccgga cctgatgtcg ggccgcgtgt cgatgatgtt cgagaacgcc
     2341 cccggcgcgg tgtcgcacat caaggcgggc aagctgcgcg cgctcgcgca gaccggccta
     2401 aagcgctcga gcgcgctgcc ggaggtgccg acggtcgcgg agtccggggt gcccggctac
     2461 gagtcgctct cctggagcgg gatcgcggtg ccggcaggga cgccgcgtgc cgtgatcgat
     2521 cggttgaaca aggatctgaa ttcaattctt gcaatggcgg acatgcggca gaagctcgcg
     2581 gagcagggcg ccgacacgat cggcggcccg cccgaagcct tcgccgcaca catccgcgcc
     2641 gagcgcgaga agtggtcgcg cgtcatcaag accgccaaca tcgtcgtcaa ctgatggggt
     2701 ttctgtcggc acgggcggat cgcgcgcgcc tttccgcgtc ggcccgggcg gcgcagcgcg
     2761 cgcgcgagct gcgcgccgcg ggcaagagca tcatcgagct ggcgcagggg gagcccgatt
     2821 tcaacacgcc cgaccacgtg atcgaggcgg cgcaccgcgc gatgcatgcg ggccagacgc
     2881 actacacgca ggtggacggc accccggatc tgaaggcggc gatcgcggcc aagttccgcc
     2941 gcgagaacgg catcgagtgc aagccggaga acgtctccgc gggcgcgggc ggcaagcagg
     3001 tgctctacaa cgccctcatg gcgacgctcg atccgggcga cgaggtgctg atcccggcgc
     3061 cgtcgtgggt ggcgtacgcc gacatcacgc atttcgccga gggcgtcccg gtgtggctgc
     3121 cgacgcggcc cgcggacggt ttcaagctct cgccgcagga cctcgagaag tcgattaccc
     3181 gaagaagcaa atggctgatg ctcaactcgc catccaaccc gaccggcgcg gtgtacacgg
     3241 ccgacgagct gcgcgcgctc gccgcggtcc tcgagcgcca cgcgtcgctg tggatcatca
     3301 gcgacgacat gtacgagcac gtgctgttcg acggccgccc cttcgccacg ctcgccgccg
     3361 tggcgccgca gctcgccgcg cgcacgctta ccgtgaacgg gctctccaag acgttcgcca
     3421 tgaccggctg gcgcctgggc tacgcctgcg ggccggtgga gctgatccgc gccatggcgc
     3481 gcatgcaggg gcagagctcg ctcaacccat cctcggtgag ccaggccgcg ggcgtggcgg
     3541 cgctgaacgg cccgatggac atcgtgaaga gccgctgcgc cgagttccag gcgcgccgcg
     3601 acttcatcgt cccccggcta aatgcaatcc gcgggctcgc ctgtgaagcg ccgcacggcg
     3661 cgttctacgt atatgtctcg tgcgcgggct ggatcggcaa gcgcacgcct caagggaacg
     3721 cgatcgcgaa cgacgccgac gtcaccgcgc acctgctgga gaacggcgtc gccctgccga
     3781 acggcgccgg ctacgggctg tcgccgtatt tccgcgtttc gttcgcgagc tcgctcgcca
     3841 acctcgagga agcgtgcaag cgaatcgacg ccgcggcgcg taagctcgcc tgatgcacct
     3901 ggtcgaacga tttgccgact gggcagtttc cttcagagag actcctctgg agaagacggt
     3961 gctgcatcat gcccgccgcg ccgtgatcga ctggcacgcc gcgctgtatc ccggaatggt
     4021 cgtgccgccg gtcacgctga tgaagaaggc cttcgccggg gagagcgaat cgccgcggct
     4081 gaaggcgctg atcgaaggca ccgccgcgca cacggtggaa gtggacgaca ttttccggga
     4141 cggcatctac cacccgggcg cgcccaccat cgccgcggcc tttgcaatca accagaacct
     4201 cgtcagggca atcgtggtgg gctatgagat ctccacccgc atcggcgcgg cgatggggaa
     4261 ggctcactac aagcactggc acaacaccgg caccatcggc tgcttcggcg cggcggccgc
     4321 ggcggccgaa gccttgcaac tgaatcgctc gcagttcgcg catgcgctcg ccacggtcac
     4381 cacattcgcc gccggcctgc agcaggcgtt ccgcatggac tcgatgtcga agccgctgca
     4441 cgcggggcgc gcggccgaag cgggcgtcgc tgcggcgctg atggcgcgcg aggggatcac
     4501 cggatcgctc gacgtcatgc agggcttcgc cgccgccatg ggcgacaagc ccgacttcga
     4561 gcggggtctc gcgacgctcg gcaaagactt ccacatcacg cgcatgacgt tcaagaacca
     4621 cgcctgctgc ggccacacct tcgctgcgat cgacggcgcg ctggagctga agaagcgcat
     4681 gcaggtaaag cccgaggaca tcgagcaggt cgaggtggcc acttataagg ccgggctcga
     4741 ggttgcgcat tacgaggcgc cgcgcacgcc ggccgaaggt cgcttctcgc tcaagtacgt
     4801 cgtcgcgacc gcgctctcgc acggcagcgt gcgcctcgcg gctttcgagc cgcagcggct
     4861 tgaagaccct gtcacccgaa aacttcttca gaggatctcg gtttcggtcg atcccgagct
     4921 cgaagcagca tttccggcgc agcgcgcggc gcgggtcgcg atccgcgcgc gcggcaagcg
     4981 cgaggagtgg ctgcagccga cgcgcatcgg ggacccggac gcgccgctct cggaccgcgc
     5041 gctggaacag aagtatttcg agctcgcgac gccggttctg ggccaggaga aggcgaagga
     5101 agttctcaaa cgcctctggc agctcgaatg acggatgtcc cggtctgccg accccctgac
     5161 aagggggtcg tgagccgcag gcgagcgggg ggtttgatct tcgcgaaaac caaacccccc
     5221 gctcgcttca ctcgctcccc ccttatcagg ggggcaagac cttgatcgat ctcgcccaag
     5281 cactcttctc gccgcgcgcg gtggcgctcg tcggggcgtc gggcgacgcc gccaaaacca
     5341 ccgcgcggcc gcagcgctac ctgaagaaac atggctatca gggccgtgtt ttcccgatca
     5401 atcccactcg aaatgaggtt ctcggccaga ggagctacgc aagagtgtcc gaagtgcccg
     5461 agcctgtcga ccacgcctac attctgaccg gcgaggtgga gcaggcgctc gaggactgcg
     5521 gcaagcgcgg cgtcgcggta gcgagcatat tcagcggcgg ctttgctgac gccgggccgg
     5581 aaggggcgaa gcgcgaagcg aagctcgtcg cgcgcgcccg cgagctcggt gtgcgcctgc
     5641 tcgggccgaa cagcatgggc gtgatcgacg ttcccgggcg cctcgcactg agcgtgaacg
     5701 cggtgctgga gatggacgcg ctgcccccgg gcacgacgag catcgtgtcg cagagcggca
     5761 ccatgctcgg cacgctgctc tcgcgcggcg cggcgcgcgg cctgggcttt ggcaggctgg
     5821 tgtcggtggg caatgaggcc gacgtcggcg tcggcgagct ggtcgacttg ctcgcggccg
     5881 acgccggcac gcgcgtgatc ctgctcttcc tggaaaccat cagggatgct caacgcctgg
     5941 cgagcgcggt tcgagctgct catctggctg ggaaagccgt ggccgcctac aagctcggcc
     6001 gctccgccct cggcgaggcc gcggcgctct cgcacaccgg cgcgctcgcg gggaccgatg
     6061 ccgccctcga cgcctacttc cgcgactgcg gcatcgtgcg cgtcgacatg ctggagacgc
     6121 tgctcgagat cccgcctctc ctggccggcc gggttccgct cgatctcggc cgtgcaggga
     6181 aagtcacggt cgtcaccacc accggcggcg gcgcggcgag cgtggtcgac cgcctcggca
     6241 tgcggggggt cgagctcgcg gcgccgatca tggacctgac gatggccgcc agcggccagg
     6301 actacggcgc agcgctggcc aaggctgcgc agacgaattg cgacgcggtg ctcgcctgcg
     6361 tcggctcatc ggcgcagttc caccccgact tggcggttca acccattgtc cagggtccga
     6421 gggtgaaacc cctgatcgcc ttcttcacgc cgcaggccga tcgctcgctg gccctggccg
     6481 cggccgcagg catcccggcc ttccgcactc ccgaagcgtg cgccgacgcg gtggcgtctt
     6541 tcctctcgtg gcgctcgccg agaaagccgc cggcgcttgc cgtcccggcg gatcttccgg
     6601 aaaacccgtt cgagctcctc gccgcgctcg gcatcccggt cgcgcgctgg gccatcgccg
     6661 aagccccggc gttcgcccac gggatcgagt atccggtggc gctcaagcgc ctggaagcgc
     6721 acaagaccga gcgcggcggg gtggtgctcg gcattcgcga ccgggcggaa ctcgaaaagc
     6781 aggcaaaaaa aatgaacgcg gaccgggtgc ttgtacagac aatgcagtcg ggcctggccg
     6841 aggccatcgt cggctatcgc gacgatgcgg tggtcggccc cgtggtgctg ctcggcgccg
     6901 ggggcgtgct gaccgagatc caccgcgact acgtgttgcg catggcgccg gtaagcgaga
     6961 gggaggcggg ggaaatgatc gaacaggtga agggattcgc cgcgatccgc ggctatcgca
     7021 acctgccgcg cggcgacctg ggggcgctcg ctcgcgccgt gtcggcgctt tccgcgctgg
     7081 ccctggtccc cggcaggccg ctgcgcgaag ccgaggtgaa tccgctcatc gtaagcgccg
     7141 aaggcgtggt ggcggtcgac gcgctggcgg cgcgcaaggg ggcggcatga tcgggttcat
     7201 cggcctgggc gtgatgggcg agccgatctg ccgcaacctc gccgcgaaga gcggcgagaa
     7261 agtgctcgcc tacgacgtcg acccggcgcc gctcgggcgt ctgaaggaag ccggcgtcga
     7321 gacggcggct tcggtgcgcg agatcgccga gcgctgcaat ttcatcttcc tgtcgctgcc
     7381 gggcgtgcag caggtgaaga ccgtctgcgt cgggccggga agcctgatgc tctacatgat
     7441 cgccggcagc tacatcatcg atctcagcac ggtcacggtc gcgcttacgc gcgatctgca
     7501 gtcgcgcttc tccgcgcgcg gcgtgtattt cgccgacgcg ccggtggcgc gcacgcgcga
     7561 ggcggcgaaa aagggcaccc tctccgtcat ggtgggcggc accgagcagg tgtacaagcg
     7621 catccggccg ctgctcgtcc acatcgccac cgacatcacc cactgcggcg gcccgggcgc
     7681 cgggcaggcg accaagctca tcaacaacat ggtgctgttc cagaacgtgg tcgcgctcgc
     7741 cgaggcgatg acgctcgcgc ggcgcgccgg cctttcggcc acgcgcgtgt tcgaggctct
     7801 catgctgggc tcggcggaca gcttcgcgct gcgccaccat ggactgaacg ccatgctggg
     7861 ggacgaatat cccgagcgcg cctattcggt cgaatacgcg ctgaaggacg tgcagtacct
     7921 gctcgagttc gccaggcagg cgcgcgtcga gctgaagggc gcgaagctcg ccatggaagc
     7981 gctcgagcgc gccgccgcgg ccggcaacgg cgcgaaatac tttccggtgc tggtcaagac
     8041 gctctgatgc cgcaggccgc tctgcgggaa attctcctcg ccgcaggact ggatttttca
     8101 gaagtcgact tcgccggcga cgacccggtc ctgcccatcc ggtatcgcgt cgccgccgcc
     8161 ggcgccgcat cgctgggagc gctcggcctc gccgtagcga agcttgccaa cagggcgcaa
     8221 cgaatcagcg tgaacgcgcg cgcggcggcg atctcgctgc gcagcgcgac gtatctgcgc
     8281 atcaacggca agcccctgcc gccggtctgg gacccgctga gcggcttcta cccggtgcgc
     8341 gacggctggg tcagcatcca ctgcaatttt ccgaaccacc gcgaggcggc gatgggcgta
     8401 ctcggcgccg gcgccgatcg cgcccgcgcc gaagccgcga gccgcgcgtg ggacggggag
     8461 gcgctcgagg acgccatcca tgccgccggc ggctgcgcag ggtttgtccg ttcccaggat
     8521 cgatggaacg cccatgcgca agcgcgggcg gtggcgcaac agcctctcct tcagatcgaa
     8581 cgcatcggcg acacgccgcc ggaattctcg aagaatgcgc gcccgctcgg cggcgtccgc
     8641 gtcctcgatc tcacccgggt gctcgccggg ccgacgtgcg cgaaaagcct ggccgagcac
     8701 ggcgccgacg tgctcaagat cagcgccgcg cacttggccg attccggcct ggtcgagctc
     8761 gacaccggga tgggaaagcg ctcggcacgc ctggatctta gaaatccggg ggaagtgaaa
     8821 gcttttcaac ggctaatccg cgaggcggac gtctttgtcc agtcctatcg gcccggcgcg
     8881 ctcgccggac gcgggttctc tcccgacgcg ctggccgagc tccgccccgg gatcgtctgc
     8941 gcttcgctga gcgcctgggg accggccggg ccgtggcgag agcggcgcgg cttcgacagc
     9001 atcgttcagg ccgtgagcgg catcgcgcag gccgcgggag gagatgcggg ctccggcgac
     9061 aagccccgcc tgctgcccgt ctcggcgatc gattacgtca gcggctacct gatggcgttc
     9121 ggggtttgcg tcgcgctcgc gcggcgcgcc accgagggcg ggagctggct ggttcgcgtg
     9181 gcgctggcac gcgtcggcaa atggatcgtc gaccgcggca cgctcgagga ttaccgcggc
     9241 ttgcccgccg atctgccgga gagcgagctc gccgcgctgt tcggcgagat ggacgcgccc
     9301 gacggccgca tccgctatct caagccggtc ctcgggcttt ccgaaacgcc gccgtatttt
     9361 gcgaggccgc cggtcccgct cggctaccat ccgcccgtat ggacttccag ctgacgcccg
     9421 agcaggcgca gttccgcgac tcggtcctcg ccttctcgaa gaagcacctc gcggccgggg
     9481 cgcgggagcg cgcccacaag cccgactatc catgggacgt cgcccggctg atggccaagc
     9541 aggatctcct cgggatcacg ctgcccgaag cggacggcgg gcagggcggc agcctgatgg
     9601 acgcggtcat cgcgatcgag gccgtggctt cggtctgccc gcgcagcgcc gacgtggtgc
     9661 aggcaggcaa cttcgggccg atccgcgtat tgggggaata cgggtcgcaa tttcagaaaa
     9721 cccggttctt gaagaagctt ctttcaggtg aagccgtcat ctccgtcggg atgacggagc
     9781 ccgaagccgg atcggcggtg accgacctcc agaccagcgc cacgcccgac ggcgacggct
     9841 ttcgcatcaa cggcacgaag gttttcacca cgcattcagc ctacgccgaa gtaatcctga
     9901 cctacgtgcg cttcggtccc ggcgtcagcg gaatcggttc cgtcctgatc gagaagagcg
     9961 cgccaggcgt ccgcaggggt aagccatcga cgttcatgtc cggcgaagaa tgggccgagc
    10021 tccacttcga cgacgtgcgt gtcccgggcg agatggtgct gctgcgcgaa ggcggattca
    10081 agaagcagat ggcaggcttc aacgtcgagc gacttggaaa cgcctcgcgc tcgctcgcgc
    10141 tcggccgcta cgccttcgag cgcgcgcgcg agtgggcgct ccagcgcaag cagttcggaa
    10201 agttgctcgc cgagttccag gggctgcagt ggaagttcgc cgacatgaag atccagctcg
    10261 acgcggctca gctgctgctt taccgcgccg ccgccaacgc cgaccgcggc atgccctcgg
    10321 ccgaggagac ggcgatcgcc aaggcccatt gcaaccgcac cggcttcgac gtcgcgaacg
    10381 aggcgctgca ggtcatgggc gcgctcggct acagccagga ggagctggtc gagtattgca
    10441 tgcgccgctg ccgcggctgg atgatcgccg gcggctcgat cgagatcctc aagaaccgca
    10501 tcgccgaggg gatcttcggc cgcagctttt cccagcgcgg atgatcaacg cggcacgctt
    10561 tctcggtttg cttttcacgg cgctggcgct ggcgccggcg ctcgcgcatc tgctcgagct
    10621 cccgaacaag atgggcctgt cgcgcgacga gtacctggtg gtgcagcaga tctatcgcgg
    10681 gtggtcgatg ctcggcgtgc tggtcttcgg cgcgctgctt tccacgctgc tgctgacgat
    10741 cctggtgcgc aagcgcccgg gcgaattcgg ggccgcgttg accgcctttc tcgccatcgc
    10801 cggcactcag gtcgtgttct ggatcttcac cttcccgatg aatcagcgga ccgcgaactg
    10861 gaccatcctt ccggatcatt ggggggcgct gcgcatgcag tgggagtact cgcatgccgc
    10921 agccgcagtc ctcaacctcg tcgcgctggt tgcggtcatc gtctcggtcc tggcgcggag
    10981 gaaaccgtga tcgaagacct ggcgcagctc gtgaatcgcg acccggcgat cgtgcgctgg
    11041 ggtcggcgca tgcacgagac cttcatggtc gaggtgggtg atacccaata cctgctgacg
    11101 gttcgcgacg gccggctcga agtcgagaag ggaccgttca cgatgcgctc gtggaaattt
    11161 gccatccggg cgagccgcga gtgctgggag aaattctggc agaagacgcc ggcgccgggc
    11221 tggcatgatc tgttcgcgct gctgcggcgc ggggatgtga agttcgaggg cgaccagcgc
    11281 gtgctgatgg cctacctgct ctacgtgaag ctggttctcg ccgctccgcg caacgcatga
    11341 agcccgaacc gaaaaccggc gaacctaaaa tcagggacag accacgtttt tcgtgcgcgc
    11401 cactggggag aaaacgtggt ctgtccctga ttatccgtgg ggcatcgtga agctcgagcc
    11461 gatcgtcggg cgctacgttc gactgaacga ctgtcgaatc tatttcgaag aggcggggca
    11521 gggcattcca ttgctctgtc tgcacaccgc cggctccgac ggccggcaat accgcggcgt
    11581 gctgaacgat gcccagatca cgaagagcta cagggtgatc gcgttcgaca tgccgtggca
    11641 cggcaagtcg tcgccgccgg cgggatggca gaacgaggat taccggctca ccaccggctc
    11701 gtacaccgcg atgatcatgg atttcatcaa gacgctcggc ctggagcgcc cggtggtcat
    11761 gggatgctcg atcggcgggc gcatcgtgct ggacctcgcg ctcaagcacg cccgcgagtt
    11821 gcgcgcgctg gtcggcctgc agaccggggc ttttgtggag cgctactacg acgcggcgtg
    11881 gctagaccac ccggaagtgc acggcggccg cgcctgcggc gcggtggctt atgggttgat
    11941 gtcgccgctc agcccggaga gcgagcgctg ggagaccgtg tggcactaca tgcagggcgg
    12001 cccgggcatc ttcaccggcg acctgtactt ctaccaggtc gagggcgata tccgcggccg
    12061 catcggcgcc atcgacacca gcgtctgccc gctgtatctg ctgaccggcg aatacgacta
    12121 ctccgcctcg ccgcgcgaca ccgaggccgc ggccgcgcgc atcaaaggag cgaagatgac
    12181 ggtcatgaag ggcctcggac atttccccat gagcgagaac ccagaagcgt ttcttggcta
    12241 tctgaggccg gtgctcgagg agattcgcaa ataagacagg cattcgccgc cgtattgctc
    12301 gcgctggcga gcgcgtccgc cggcgcgttt cccgacaagc c
//
LOCUS       DATBSZ010000179         2694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_781207, whole genome shotgun sequence.
ACCESSION   DATBSZ010000179 DATBSZ010000000
VERSION     DATBSZ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781207"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2130
                     /locus_tag="VIG70_11600"
     CDS             <1..2130
                     /locus_tag="VIG70_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8251245.1"
                     /translation="VQPNLILDDRYELLAPDRVFLSVAAAQSLGLGKGDTLPLVVGQT
                     ITELKVAGVLSSLRGVSALTDIATAQWRFGRLGELNRVDVRLSPGAERQRVSAAIQAL
                     LPPGSHVIALETLEEASAYPSRSYRVNLNVLAMVALFTGGFLVFSAQALEVARRRGEH
                     ALLRVLGLKRKQISLLVLEEASLLGAVGAVLGLLLGYALASLAVRASGADLGAGMFRG
                     GSPQIEIGAAGAVAYFLAGILVAVLGALLPALDAARTPPARALKAGDEQTLFERIAPL
                     WPGVVLLAAGGALAQLGPVHGVPLFGYAAIGCLLVGAIALVPIISAFLFKHLPLPTYP
                     PMRLALGQLRAAPGQAAVSLAAIVASFSLMTAMAIMVASFRQSVDDWLTTVLPAELYF
                     RSTHAGDTGILEAGFEERVRALPQVARADFLRSGRILLDAARPPLVLIARDRADRTFP
                     ILGERYERRTGDPPAVWISEAVSDLYGFAPGHRLGLPMLGKSHPVVVGGVFRDYARQH
                     GAVLIERSDYVALTGDRAVNDGALWLAPGTTPAAAMEALSALPGAAQLEMAQPGEIRE
                     VSLRIFDRSFAVTYAMEAVAVLVGLFGLSSSLGAIVLARRREFGVLRHLGLTRGQIRA
                     MLAAEGGLLALVGSAAGLAAGAAISLVLVYVINRQSFHWSMELHPPYLLLLGFVFILV
                     FLAILTAVISGKEAMGMAPVRAVREDW"
     gene            complement(2173..>2694)
                     /locus_tag="VIG70_11605"
     CDS             complement(2173..>2694)
                     /locus_tag="VIG70_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEY8251246.1"
                     /translation="FTIEKPVETPPDRVEKSAAAAALSLPESSVLEAWFASGGVRFAF
                     VRLADKAAVDRVELDRAAWVRHFKNAWASQLYFFTGEKEVYARMFAPALGIEEDPATG
                     SAAVGLASVLASRLPAADGVFTWRIDQGVAMGRASRLEASAEKRGGRVVRVKVGGVTV
                     LSAEGSMNVPANY"
BASE COUNT          340 a          960 c          929 g          465 t
ORIGIN      
        1 gtgcagccga acctgatcct cgacgatcgc tacgagctgc tcgcgccgga tcgcgttttt
       61 ctctcggtcg cggcagccca gagtcttggc cttggcaagg gcgacacgct ccccctcgtt
      121 gtgggtcaaa ccatcacaga attgaaggtc gctggcgtgt tgtcctcgct gcgcggggtg
      181 tccgcgctga cggacatcgc gaccgcgcag tggcgcttcg ggcgcctggg cgagctgaac
      241 cgcgtggacg tgcgcctctc gcccggggcc gagaggcaaa gggtgtcggc ggcgatccag
      301 gcgctcctgc cgcccggcag ccacgtgatc gcgctcgaga cgctggagga ggcgagcgcg
      361 tatccctcgc gctcgtaccg cgtgaatctg aacgtcctgg cgatggtggc gctgttcacc
      421 ggcggcttcc tcgtgttctc ggcgcaggcg ctggaggttg cgcgccgccg cggcgagcac
      481 gcgctgctgc gggttctggg gttgaagaga aaacagattt ccctgctggt gctggaggag
      541 gcctcgctcc tcggcgcggt cggggcggtg ctcgggctcc tcctcgggta cgcgctcgcg
      601 tcgctcgcgg tgcgggcttc cggcgcggac ctgggcgccg ggatgttccg gggcggctcg
      661 ccgcagatcg aaatcggcgc ggccggcgcg gtcgcgtatt ttctcgccgg catcctcgtc
      721 gccgtcctcg gcgcgctcct cccggcgctc gacgccgcgc gcacgccgcc ggcccgcgcg
      781 ctcaaggcgg gggacgaaca gacgctgttc gagcgcattg cgcctctttg gccgggtgtc
      841 gtcctgcttg ccgcaggtgg cgcgctcgcg caactcggcc cggtgcacgg cgtgccgctt
      901 ttcggctacg ccgccatcgg ctgcctgctc gtgggcgcaa tcgcattggt accaatcatt
      961 tctgctttcc tgttcaagca tcttcccttg ccgacgtatc cgccgatgag gctcgcgctc
     1021 ggccagttgc gcgccgcgcc cggccaggcg gcggtgagcc tggccgcgat cgtggcgagc
     1081 ttctcgctga tgacggcgat ggcgatcatg gtggcgtcgt tccggcaatc ggtggacgac
     1141 tggctgacga ccgtgctgcc ggccgagctt tatttccgct ccacgcatgc cggggacacg
     1201 ggcatcctcg aggccgggtt cgaagagcgc gtgcgcgcgc tgccgcaggt cgcgcgcgcc
     1261 gatttcctgc gcagcggccg catcctgctc gatgcggcgc gcccgccgct cgtgctcatc
     1321 gcgcgcgacc gcgccgacag gacgtttccc atcctgggcg agcgctacga gcgccgcacg
     1381 ggcgacccgc ccgcggtctg gatcagcgag gccgtttcgg acctgtacgg cttcgctccc
     1441 ggacaccggc tcggcctgcc catgctcggc aagagccacc cagtggtcgt cggcggcgtc
     1501 ttccgcgact acgcgcggca gcatggcgcc gtcctcatcg agcgctccga ctacgtcgcg
     1561 ctgaccggcg atcgcgccgt gaacgacggc gcgctctggc tcgcgcccgg aaccaccccg
     1621 gcggcggcga tggaagccct gagcgctcta ccaggagccg cgcagctcga gatggcgcag
     1681 cccggcgaga tccgcgaagt ctcgctgcgc atcttcgacc gcagcttcgc cgtcacctac
     1741 gccatggaag ccgtcgcggt gctggtcggc ctgttcggcc tgtcctcgag cctcggcgcg
     1801 atcgtcctcg cccgcaggcg cgagttcggc gtgctgcgcc acctcggcct gacgcgcggc
     1861 cagatccgcg ccatgctcgc cgcggaaggc ggcctgctcg cgctggtggg ctcggcggcc
     1921 ggccttgccg caggcgcggc gatcagtctg gtgctggtct acgtcatcaa ccgccagtcg
     1981 ttccactgga gcatggagct ccatccgcct tacctgctgt tgcttggatt cgtcttcatt
     2041 ctcgtcttcc ttgcaattct cacggccgtc atctccggca aggaagcgat gggcatggcg
     2101 ccggtgcgcg ccgtgcgcga ggactggtag ttcggggacg gagcccgaac tagttagggc
     2161 tccgtccccg aattagtagt tggcgggcac gttcatggag ccctctgccg acaacaccgt
     2221 aacgccgccg actttcacgc gcacgacgcg cccgccgcgc ttctccgccg aggcttcgag
     2281 cctgctcgcg cggcccatgg caacgccctg gtcgatgcgc caggtgaaca cgccgtccgc
     2341 ggcggggagg cgcgaggcga gcacgctggc gagccccacc gccgcggatc cggtcgccgg
     2401 atcctcctcg atgccgagcg ccggcgcgaa catgcgcgca tagacctcct tctcgccggt
     2461 gaagaagtag agctgcgacg cccaggcatt cttgaaatgc cggacccaag cggcgcgatc
     2521 gagctctacc cggtcgaccg cggccttgtc cgcgagccgg acgaatgcga agcgcacccc
     2581 gccgctggcg aaccaggctt ccaggaccga gctttccggg agcgaaagcg ccgcggctgc
     2641 ggcggatttc tccacccggt ccgggggcgt ctcgaccggc ttctcgatcg tgaa
//
LOCUS       DATBSZ010000180         5711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_917016, whole genome shotgun sequence.
ACCESSION   DATBSZ010000180 DATBSZ010000000
VERSION     DATBSZ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917016"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VIG70_11610"
     CDS             complement(<1..452)
                     /locus_tag="VIG70_11610"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341793.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY8251247.1"
                     /translation="MRTDNLIIFDTTMRDGEQSPGASMTRDEKLRIARALERMKVDVI
                     EAGFPASSNGDFEAVRAIANVVKDSTVAGLCRANDRDIQRGIDALKGAKSWRVHTFIA
                     TSALHMEKKLRMSPEQVLEQARLSVRFARKHCPDVEFSPEDGSRSDLD"
     gene            complement(610..2019)
                     /locus_tag="VIG70_11615"
     CDS             complement(610..2019)
                     /locus_tag="VIG70_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251248.1"
                     /translation="MHAALAALAFAPAGAFAHGFGARHELAVPVWLFIVGAALTVTLT
                     FVAAAAFAGSRVERYAAARCEFSRAFIPGWTIAALRIAGVVALVLVLVAGFIGSRDPN
                     RNIAPTLVWIVGWVGFSYVAMLLGNLWPVVNPWRTLHELFARAPSPPRSWPRSLGSWP
                     AVAALLAFGWLELVFPFRATPPVLAGLVLAYSVVTWAGMARYGAQAWLEHADPFHRVF
                     ELFSRFAPFAAAPNGRLVVRPYAAGLWPREPERLPMAHVAFILAMLAIVLFDGFQASV
                     HWVAIEDAVHALDPQLGDGAWLALHTAGLLAMWLVFLGLYLAACRLAKAAANASLTTL
                     DYARAFALTLVPIAVGYHFAHTFTYLIVQGQSLAFLVSDPFGFGWNLFGTAGATVDDA
                     IIATRTAWYLALGAIVVGHTISVVLAHAAADRLLAGRGRALRALVPMTALMVLYTVIS
                     LQILAEPLVRYSGPQETII"
     gene            complement(2025..3080)
                     /locus_tag="VIG70_11620"
     CDS             complement(2025..3080)
                     /locus_tag="VIG70_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251249.1"
                     /translation="MMRFTHLIAAAGTAAFLSIGAATAQSDVPGKRLEEIYAMSPERM
                     GTTSKWIRAKYDQLLVHIRSIKDPQIRALVLDMVTAPRSTVFNATAERDAFLIAPAAG
                     GPGHHFYPGGLAVHAVENIEISLGWADAMAKVHGVDNTNRDIIIAALTLHDWAKVWYQ
                     WDPATGTIKRPDWFPAYWGGEQGIAKWRWMGGHGAIVYAELMKRGAPADLIIATAAAH
                     FDPFWDVDKVDGKEGLNAALAEAAKLARVPAPKVDPAKRMAEWWMIVYSDGSWSYSHY
                     IAGQFAHAWAREVAKDLGVDPKSPQANKLANFALTRLSDFKLYSQYQAAGYDASVPKK
                     AILGVLRESGAHEMAAR"
     gene            3140..3841
                     /locus_tag="VIG70_11625"
     CDS             3140..3841
                     /locus_tag="VIG70_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4743 domain-containing protein"
                     /protein_id="HEY8251250.1"
                     /translation="MSFSPEAYVPLLFQGARIGWLRPELAARVRAWSSVFSASAERVT
                     LLQPDALAPVLEQLAQEGFIPGWRNERYRIADLFDIERAAARPFGLTTRAVHVNGISS
                     DGKMWLARRAHTKPIDPGVLDNLVGGGIATGLSVEQTLVKEAWEEAGIANELARTATR
                     GGTIRVLREVPEGVQSELIESYDLALPAGFQPKNQDGEVSEFRLVSFSEIENQTMTFE
                     AALVARDCLSRRRAP"
     gene            complement(3819..>5711)
                     /locus_tag="VIG70_11630"
     CDS             complement(3819..>5711)
                     /locus_tag="VIG70_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8251251.1"
                     /translation="WPLLRIDDLQGALWFPADGKANPADLAMSLAKGARNLGVQVREV
                     VQVSSVNKKNGFVSGVETSEGPISCEILVNCAGQWARALGARDGVNIPLHSAEHFYIV
                     TEAIEGVHPMLPVMRDPDGFIYFKEEVGGLVMGGFEPVAKPWGMEGIPDKFEFQLLPE
                     DWDQFQPLMEAAMHRVPALETAGVKMLLNGPESFTPDGNFILGEAPEIRNYFVAAGFN
                     SAGIANAGGAGKLLAEWIAGGEAPMDLFEVDVRRFGAFAADAAWLKARTVETLGLHYA
                     MRWPRHELESGRPQRVSPLYERLKRKGAQFGSKFGWERANYFGAKMQYTLGRPNWLAA
                     VIEEQRAIRNTVGILDQTSFGKLRVRGRDALALLERVCANHIGKLAYTAMLNRKGGFE
                     SDVTVQRWADDDFFIVTGSAQPVRDRAWLLRNRKPGEEATVEDVTAEWSVISILGPNA
                     RHLLEKTDIGSARAVPASYVGGPGSEIYVPVAEAPSLYDRLWAAGKALGLRDCGYYAL
                     DALRIEAGRRAFGPELSPDCTPYEAGLAFAVRPENRRPPERLSKRLLMFTFPETELFA
                     WGGEPILRDGKPVGELSSVGYSATFGCMVGMGFVHSEDIGGNYEIEVSGTKVAAKASL
                     KAPFAG"
BASE COUNT          963 a         1929 c         1932 g          887 t
ORIGIN      
        1 aagtcgaggt cggagcgcga gccgtcctcg ggcgagaact cgacgtccgg gcagtgcttg
       61 cgcgcgaagc gcaccgagag cctcgcctgc tcgagcacct gctcgggcga catgcgcagc
      121 ttcttctcca tgtgcagcgc gctggtggcg atgaaggtgt gcacgcgcca ggacttcgcc
      181 cccttgaggg cgtcgatgcc gcgctggatg tcgcggtcgt tcgcccggca cagcccggcc
      241 accgtcgagt ccttgactac gttggcgatg gcgcgcaccg cctcgaagtc gccgttggac
      301 gaagccggga agccggcctc gatcacgtcg accttcatgc gctccagcgc gcgcgcgatg
      361 cgcagcttct cgtcgcgcgt catcgaagcg ccggggctct gctcgccgtc gcgcatggtg
      421 gtatcgaaga tgatcaagtt gtcggtacgc atggaatggg tccttggcct ttttcaggcc
      481 tggtcgcaac acggattgta ggcgggagta tttagcgcgc ggggcgcagc agcaggccgc
      541 ccagcgcgag gagcgcgagc gagagaagag cgagaatcgc ctgttgcatg gaacgactat
      601 agcccatttc taaataattg tctcctgagg gcccgagtag cgcaccagcg gctcggcgag
      661 gatctgcagg ctgatcaccg tgtagagcac catcagcgcc gtcatcggca cgagtgcgcg
      721 caaggcgcgc ccgcgccccg cgagcaggcg atcggcggcg gcgtgcgcca gcaccaccga
      781 gatcgtgtgc ccgacgacga tcgcaccgag cgccaggtac caggcggtcc tggtcgcgat
      841 gatcgcgtcg tccaccgtgg cgccagcggt gccgaacagg ttccagccga agccaaaagg
      901 atccgacacc aggaatgcca ggctctggcc ctggacgatc aggtacgtga aggtgtgcgc
      961 aaagtggtaa ccgacggcga tcgggacgag ggtgagcgcg aaggctcgcg cgtaatcgag
     1021 cgtcgtcagc gaagcgtttg ccgcagcctt cgccaggcgg caggcggcca gatagagccc
     1081 gaggaacacg agccacatgg ccagcagccc ggccgtatgg agcgcaagcc acgcgccgtc
     1141 gccgagctgc gggtctaggg cgtgcaccgc gtcctcgatg gcgacccagt gcacgctcgc
     1201 ctggaagcca tcgaacagca cgatcgccag catggcgagg atgaacgcca cgtgcgccat
     1261 tggcaggcgc tcgggctcgc gcggccacaa tcccgcggcg taggggcgca cgaccagcct
     1321 gccgttcggg gcagcggcga aaggcgcgaa ccgcgagaac agctcgaaca cgcgatgaaa
     1381 tggatcggcg tgctcgagcc aggcttgcgc gccgtagcgc gccatgcccg cccaggtcac
     1441 cacgctatag gccagcacga gcccggcgag caccggcggc gtcgcgcgga acgggaacac
     1501 cagctcgagc cagccgaacg cgagcaatgc cgccaccgcg ggccacgacc ccaggctgcg
     1561 cggccaggat cgcggcggcg acggcgcccg cgcgaagagc tcatggaggg tgcgccacgg
     1621 gttcaccacc ggccacaggt tgccgaggag catcgccaca taggaaaagc cgacccatcc
     1681 gacgatccag accagcgtcg gagcgatgtt gcggttcggg tcgcggctgc cgatgaatcc
     1741 ggcgacgagc accagcacca acgcgactac gccggcgatc ctcagcgccg cgatcgtcca
     1801 gcccgggatg aacgcgcggg agaactcgca gcgcgcagcc gcgtaacgct cgacgcggct
     1861 gccggcgaag gcggcggcgg cgacaaaggt gagggtcacg gtcagcgcgg cgccgacgat
     1921 gaacagccat accggaaccg caagctcgtg gcgcgccccg aacccatggg cgaacgcgcc
     1981 cgccggcgca aatgccaacg ccgcgagcgc ggcgtgcacc gcgttcagcg cgctgccatc
     2041 tcgtgcgcgc cggattccct gagcacgccg aggatcgcct tcttcggcac cgacgcgtcg
     2101 tagccggcgg cctggtactg gctgtagagc ttgaagtcgg acagccgggt gagagcgaag
     2161 ttggcgagct tgttggcctg cggcgacttg gggtcgacgc cgagatcctt cgccacctcg
     2221 cgcgcccagg catgggcgaa ctggccggca atgtagtgcg aatacgacca cgagccgtcc
     2281 gagtaaacga tcatccacca ctcggccatg cgcttcgccg ggtccacctt cggcgccggc
     2341 acgcgcgcga gcttggccgc ttccgccagc gccgcgttca gtccttcctt gccgtcgacc
     2401 ttgtcgacgt cccagaaggg atcgaagtgc gccgccgccg tcgcaatgat caaatcggcg
     2461 ggcgcgccgc gcttcatcag ctcggcgtac acgatggcgc cgtggccgcc catccagcgc
     2521 cacttggcga ttccctgctc gccgccccag taggcgggaa accagtccgg ccgcttgatt
     2581 gtccccgtcg ccgggtccca ctggtaccag actttcgccc aatcgtgcag cgtgagcgca
     2641 gcgatgatga tgtcgcggtt ggtgttgtcc acgccgtgca ccttggccat ggcgtccgcc
     2701 cagccgagcg agatctcgat gttctcgacc gcgtgcaccg cgaggccgcc ggggtagaaa
     2761 tggtggcccg gcccgccggc ggcgggcgcg atgaggaagg cgtcgcgctc cgcggtggcg
     2821 ttgaacaccg tcgagcgcgg cgcggtcacc atgtcgagca ccagcgcgcg gatctgcggg
     2881 tccttgatcg agcggatgtg cacgagaagc tggtcgtatt tcgcgcggat ccacttgctg
     2941 gtcgtaccca tgcgctcagg agacatggcg tagatttctt cgaggcgctt gcccggcacg
     3001 tctgattgcg ctgtggcggc gccgatggaa aggaacgcgg ccgtgccggc tgccgcaatc
     3061 aagtgcgtga accgcatcat cgacctcccc gttggatgac tgccgatcga gtctagcagg
     3121 ctgctaaact ttgcgcgcct tgagcttcag tcctgaggcc tatgtgccgc tcctgtttca
     3181 gggcgcaaga atcggctggc tgcgcccgga actcgcggcg cgcgtgcgcg cctggtcctc
     3241 cgtattctcg gcgagcgcgg agcgcgtaac gctcctgcag cccgatgcgc tggcgccggt
     3301 gctcgagcag ctggcgcagg aaggcttcat cccgggctgg cgcaacgagc gctatcgcat
     3361 cgccgacctt ttcgacatcg agcgcgccgc ggcgcgcccg ttcgggctca cgacccgcgc
     3421 ggtgcacgtg aatggaattt cttccgacgg caagatgtgg ctggcgcgcc gcgcgcacac
     3481 caagccgatc gatcccggcg tgctcgacaa cctggtgggc ggcggcatcg ccaccggcct
     3541 ttccgtcgag cagacgctcg tcaaggaagc gtgggaggaa gcgggcatcg ctaacgagct
     3601 cgcgcgcacc gcaacgcgcg gcggcacgat ccgggtcctg cgggaggttc ccgaaggcgt
     3661 gcagtccgag ctgatcgaga gctacgacct cgcgctgccg gcgggcttcc agccgaagaa
     3721 ccaggacggg gaagtgtcgg agttcagatt ggtttcattt tctgaaatag aaaaccagac
     3781 catgaccttc gaggcggcac ttgtcgcgcg cgattgcctt agccggcgaa gggcgccttg
     3841 agcgaagctt ttgccgcgac cttggtgccg gaaacctcga tttcgtagtt gccgccgatg
     3901 tcttccgaat gcacgaagcc cataccgacc atgcagccga acgtcgcgct gtagccgacc
     3961 gaggacagct cgcccaccgg tttcccgtcg cgcaggatcg gctcgccacc ccaggcgaag
     4021 agctccgtct cggggaaggt gaacatcagg agccttttcg aaagcctttc cggcggccgg
     4081 cgattctcgg gccgcacggc aaaagcgagc cccgcctcgt aaggcgtgca atcgggcgag
     4141 agctccgggc cgaacgcgcg ccgccccgcc tcgatgcgca gcgcgtcgag tgcgtagtaa
     4201 ccgcagtccc gcagtccgag agcctttcca gccgcccaaa gccggtcgta gaggctgggg
     4261 gcctccgcca ccggcacgta aatctcgctg cccggcccgc cgacgtagga cgcggggacg
     4321 gcgcgagcgc ttccgatgtc ggtcttttcg agcaaatgtc ttgcgttggg cccgagaatg
     4381 gaaatcaccg accactcggc agtgacgtct tcaaccgtcg cctcctcgcc gggcttccgg
     4441 tttcggagaa gccaggcgcg gtcgcgcacc ggctgcgccg agccggtgac gatgaagaag
     4501 tcgtcgtccg cccagcgctg cacggtgacg tcgctctcga agccgccctt gcggttgagc
     4561 atcgcggtgt aggcgagctt gccgatgtgg ttggcgcaca cccgctcgag gagcgccaga
     4621 gcgtcgcgcc cgcgcacgcg cagcttgccg aacgaggtct ggtcgaggat gccgaccgtg
     4681 tttcggatcg cgcgctgctc ttcgatcacc gcggcgagcc agttcgggcg gccgagcgtg
     4741 tactgcatct tcgcgccgaa atagttcgcg cgctcccagc cgaacttgga gccgaactgg
     4801 gcgcctttgc gtttcaatct ctcatagaga ggagaaaccc tttgcgggcg gcccgattcg
     4861 agctcgtggc gcggccagcg catggcgtag tgcaggccga gcgtctcgac ggtccgcgcc
     4921 ttgagccatg ccgcgtcggc tgcgaacgcg ccgaagcggc ggacgtcgac ttcgaacaag
     4981 tccatcggcg cctcgccgcc cgcgatccac tcggcgagca gcttgccggc gccgccggcg
     5041 ttggcgatgc ccgcagagtt gaagccggcg gcgacgaagt agttgcggat ctccggcgcc
     5101 tcgccgagga tgaagttgcc gtcgggcgtg aagctctccg gcccgttcag cagcatcttc
     5161 acgcccgcgg tctcgagcgc cggcacgcgg tgcatcgcgg cctccatcag cggctggaac
     5221 tggtcccagt cctcgggcag gagctggaac tcgaacttgt cgggaatgcc ctccatgccc
     5281 cagggcttgg ccaccggctc gaagccgccc atcaccagcc cgccgacctc ctccttgaag
     5341 tagatgaagc cgtcggggtc gcgcatcacc ggcagcatcg ggtgcacgcc ctcgatcgcc
     5401 tcggtgacga tgtagaagtg ctcggcggag tgcagcggga tgttgacgcc gtcccgcgcg
     5461 ccgagcgcgc gcgcccactg cccggcgcag ttgacgagga tctcgcacga aatcgggcct
     5521 tcgctggttt cgacgccgct gacgaatcca ttcttcttgt tcaccgaaga aacctggaca
     5581 acctcgcgga cctgcacacc gagatttctc gcgcccttcg ccaggctcat cgccaggtcc
     5641 gccgggttcg ccttgccgtc ggccggaaac cacagcgcgc cctgcaggtc gtcgatgcgc
     5701 agcagcggcc a
//
LOCUS       DATBSZ010000181        27375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_29892, whole genome shotgun sequence.
ACCESSION   DATBSZ010000181 DATBSZ010000000
VERSION     DATBSZ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 27375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27375
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29892"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VIG70_11635"
     CDS             complement(<1..1005)
                     /locus_tag="VIG70_11635"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEY8251252.1"
                     /translation="MRLLDIFIATLIVFPWVTEWGSFEGTSMELPDYGLPLLIVALVV
                     EAVLRWRRVSWRARLWSSAAVAASAALLGWLFENHRDVSTRAIWHLTHGPSAVLVVAL
                     VAAAIRRYSSEPWEKSLFVRLGIKLGRAWLGALERAGPRPLWVVTAVVALAWFCVSLL
                     RHWALDSHGYDLGIFTNVMWNLTHGNGYVSAVKGGINLFADHQSPTYWLLAPVFWLVP
                     RPETLVFVQALGLAAGGPALYYLARARFGPGPLGQPHWAPAALPWLYWAYLPTRNANA
                     FDFHPEVFMLPLFLWAFVGFASDRRWAKALGFAALVAALGTKESAPVVAVGIGVAWAL
                     T"
     gene            complement(993..1709)
                     /locus_tag="VIG70_11640"
     CDS             complement(993..1709)
                     /locus_tag="VIG70_11640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8251253.1"
                     /translation="MSERDYHDRHYEAEAALFDTPLFRRVHDRAARQFLRIARAGRAH
                     RILSLGCGDGSIERRLAPHVGTVLGVDISPVAIAQARAKSAQFANLAFEVAGKWQGDF
                     DIVAAFAFLHHLDDAAIRDTLRAARRALRPGGLFYSSDPSRRRLVGLFRGLVRKTYER
                     YHSPDERELDARALAAMAGEAGFLEVRTGYSDYFLGPLAWLAPGTPRFLAPGLEALDS
                     LALNLPLARRYASSFYLCAS"
     gene            complement(1706..3439)
                     /locus_tag="VIG70_11645"
     CDS             complement(1706..3439)
                     /locus_tag="VIG70_11645"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEY8251254.1"
                     /translation="MRALLPLLCLPAAICAAWTVVAGKDLNWDLLNYHYYAPFQLLEG
                     RLQQDFFAASAQSYLNPIGYLPFYLMVSSGWHSVVVSAALAVAHGLSISLLYAIAWGL
                     FAHLPRRDRIVLSCLAAALGAASAVFWATVGTSFLDPLLVPFVLAGLLLLLHRQQPFV
                     AGLLFGLAAALKYSNAIFALAALPLAFLSGRRAAAAYVAGGALAVALFAGPWLALMLR
                     EFGNPLYPGLSAGSGRFGPPDLVSALILPFRMVMLDRSLYAETFAPDLRFAALAVLAL
                     ALPAYRKSVGDWRLLGFFALAYVAWLATSGNARYGMALLLLAGVCLAYLAERLVPLRI
                     ARIGLGVLLALQLVTAIVAAPPRWFIAEPWSKQWFPYDVPPQALREPALYLTLETLPM
                     AVVAPFVHPDSSFVNVRGQRTLAPDSPRLAALAERHRGRVRVLGRALEHLDGRTLSWI
                     GYRLDSADCFAIEWRRDDADALSRGANWISRTPPSSEPLSVASCALRPAPIDSALLEQ
                     ERRASALFDRIEEGCPRLFRGQTAVTEPFGLGGWARHYVDLDARMETIGAQVVLNRYR
                     AGEIVDLAPCR"
     gene            complement(3436..5433)
                     /locus_tag="VIG70_11650"
     CDS             complement(3436..5433)
                     /locus_tag="VIG70_11650"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEY8251255.1"
                     /translation="MQAGAVALALFALYAVTTPRTVSLEDDSLFVLSCYFLGIEHPPG
                     YPLFTLIGHLFTYLPVGSVAYRVHLASALFGALSAGAAWLCMRRLIPGVLPAYVAALA
                     LGLSPVFWSQSIIAEVYTLNTFFLLLLVYLGLDAERRPGVLPWMALVFGLSLANHYPL
                     MLLAAPAFAVLLWPLRAELLRRIGLLLILVVVGLLPYAWLVRRSWTGLPISFYGELDS
                     VTEILFFLSRAGYAEVDHSPTAGWLDKVRYLTFVGRELLVQFAVIGTVVAAAGFALQW
                     RTLGRRIAAFLTICFLMPSVVLVLLLGFDYDVFRKHIFHVYPLPAYAIGALWLGLGFG
                     WLVERYALPRARALAAGAALLALVAAVGAHVNLRANHEWAARYAQTVLKLLPPNAVVW
                     GQGDADLLPMAYFHMVEEMRPDITLYQAQGLILGNRLFHPLRTDQQIADRILREFIEE
                     QTVPVVFTLDAYTGNARRDRWLYIEVDKSSTDSKRITVDLPEEAVRFFEDAIAKESIG
                     NAWIAYFQSELRRRYGSVLGMSLTPGQAPEDARTRRHLELLGEDFYGALGLADGLLNN
                     PRGYSAGAVAAYLDRAGALLPSDAPKQHVAGYFLLRGILRANAQDGPGAIRDLETAFS
                     LWPSPKSGAIHPLETIYRDTGNQAALQDLQNRVSQLKRKGR"
     gene            complement(5493..6587)
                     /locus_tag="VIG70_11655"
     CDS             complement(5493..6587)
                     /locus_tag="VIG70_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8251256.1"
                     /translation="MPELTVVVPAFGERDNIAPLLGALERALAGLDWETIIVVDDAFD
                     GTEDVVRGRAQEDRRVRCLHRIGRRGLASACIEGMLASSAPYVAVIDADLQHDETLVP
                     KLLEAVKREGADVVVASRYMQGGSTGELAAERVRISRLASALSQLLCKDLTDPMSGFF
                     LVRRSFLERVVRRLYGRGFKILLDLVAAARGEVRVAELPYRMRSRQHGESKLGARVIA
                     EYFMLLLYHMTGRFLPARFFLFGAVGVTGVGVHLAVLTAAFQATGGNFLASQALATWV
                     AMTSNFFLNNMFTYGDQRLRGRRLWRGLLSFYVACGIGAFINLAVAEWLFLQSVAYWA
                     AGVAGALIAALWNFYTTASLTWGGSNARKP"
     gene            complement(6580..8826)
                     /locus_tag="VIG70_11660"
     CDS             complement(6580..8826)
                     /locus_tag="VIG70_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251257.1"
                     /translation="MRPLPAGLGGLAALVFAMFFDVLIAPGPRVLGVDNTDLALHFLH
                     WRNFGFGELARGNLALWNPHIYGGVPFFGGMQSALLYPPNWLFLALPLPLAANWSVAL
                     NVWLLGAFMYLWALRRGLQPFAAFVAAALAMFCAPHFLRVYAGHIAGMAAMAWVPLVF
                     LALDAWIDSRRPRWLLAGMAAVAMQIFAGHPQTVYLTALTAGLYSVLRMQILGVASLY
                     AGGASLAAVQLLTGFQATAETVRDRPMSLELAKYFSFPTENFLTLLAPGFFGDVATHP
                     YWGRWYLWEASAFIGVAGLALAAYGIARGQLANRVPLLVTAGVAAVLALGDNSPLFPL
                     LFDWLPWFDRFRGAGKFIFITALILILFAGSGLDRLLRGRDVPRGAVWAAGAGALALG
                     VAGVLTQSVDWRPVAGAVLATGQTYLDHARYADAGFLFASRSFAALGLMVAALTLAAA
                     AGLLLWARKEARAAVVLGVLAVAEVFAYARLNRPTFDSTHIFIPELAGFLRRNPGEYR
                     ILNLARPNAAISMGAFDAWGYDPGVTRRYAEFISWSAGEDPEKNNIPHVIFRQFHPLL
                     AMVRVKYVVAFDDGVMTIHPGGTPPLGRLELIGSHQVRAGRAAILQAMGSPGFDPSRE
                     VILEREPAPVPVAGETPGRARILREGSDFMEIEAELERPSVLLVTDAWTPAWRAVPLD
                     AGDTRRYEIMPANYALRAVALDRGRHRLRLEYTPWEFRLGAAISVFASIGWLAAAWFA
                     WKRGGRIA"
     gene            complement(8823..9971)
                     /locus_tag="VIG70_11665"
     CDS             complement(8823..9971)
                     /locus_tag="VIG70_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8251258.1"
                     /translation="MTELTILMPCLNEAATVGRCIAKARGFLERARIAGEVLVADNGS
                     DDGSAALAQQAGARVVEVAERGYGAALAAGIAAARGRYVIMGDADDSYDFGRLELFVE
                     KLRQGYPLVMGNRFRGGILPGAMPPSHRYLGNPLLSFVGRLFFGARVGDFHCGLRGFD
                     REAVRSLDLRTPGMEFASELVVKAALAGWRIAEVATTLHPDGRGRPAHLRRWRDGWRH
                     LRFLLLFSPRWLFLYPGLALLALGIGLMSALYFTPLHILGAGLDIHSMLYASAAALLG
                     LQLCLFALFARVSAQNAGLLPRQPGLDRLLKVLTLERGLLAGLFMALGGLAWSAAAFW
                     QWRETGFGPLDPRVVMRDTIPATALLVGGMELMLASFLLSVLRLKTDG"
     gene            complement(10042..10518)
                     /locus_tag="VIG70_11670"
     CDS             complement(10042..10518)
                     /locus_tag="VIG70_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEY8251259.1"
                     /translation="MKQLQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRAKMSEVI
                     LGMSACRTSITEVYQSGPAGLIAPNGWGCEYGGTTATKYVAAVNTSANGNVSATVQGV
                     SLSVNGSVVTLTPLSSPNTTATVTPGNSMALFGWRCGSTTDGTNVPGKYLPGSCRG"
     gene            complement(10692..13667)
                     /locus_tag="VIG70_11675"
     CDS             complement(10692..13667)
                     /locus_tag="VIG70_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251260.1"
                     /translation="MQLGATVRPLILLPAAFVLFTVSGFAGLIYESIWSHYLKLFLGH
                     AAYAQTLVLAIFMGGMALGAWGASRISARWRDLLLAYAVIEALIGLISLVFHSVFVAT
                     TAFAFEAVIPALGSPAAVHALKWTLAAALILPQSVLLGMTFPLMTGGVLRLRPERAGY
                     AIAMLYFTNSLGAGAGVLASGFYFIELAGLPGTLTIAGVLNLAVAAGVMFLPRRSDTA
                     PAPSVAAENAPVPMGRLLLAVAALTGMSSFMYEIGWIRMLALVLGTSTHAFELMLSAF
                     ILGLAFGGLWVRRRIDAARDTLRLLGLVQVAMGIAALATLPVYVASFEFMQLTMQSLA
                     RTPGGYLALNAVSHGICLAVMFPAAFCAGMTLPLLTASLMRAGAGERAIGQVYAANTA
                     GAIAGVVAAVHLGLPLLGLKGLIIAGAAIDLALGVVLLGVGRRPYAAAAVSAAAVCFA
                     IIGVRLDPLHMASGVYRSNVLMRQGGGEEVRLHLDGKTASVSITGGKELIALRTNGKS
                     EGAIRVDGAGVTHDEITMTLTGALPFFLLPEARRIANIGFGTGLTTHVMLGSDRVESV
                     DTIEIEPAVVTAAAALRPFNARAFDDPRSRLHFEDAKTYFSARGERYDVIVSEPSNPW
                     VSGVASLFSSEFYRDVRRHLRDGGLLVQWVNVYELSPVLLATIIAALDAHFSDYEIWM
                     PNAGDLLVVAVHKGTLRAPDARAFDSPRLRADLERFGIRSLDDLLLHRVAGRSAMHPY
                     FASFRVPANSDFYPVLDLNAAKARFLHRPADEEIPRLLEAGFPLLDLFDRPRVGQPDP
                     ARLSPASRPWLVRAGYAAQARAAGAYLLSGRLAELESLSAAVSGDLVLLRTALVDCSV
                     RVPASTLRRVLLDAAWLVNQHLGRAERQAVWRLLAGSPCRTRLTETERQWLRLHAALG
                     AENGGEIASAAGQILEADASLPAEFAAYALAAHMAGLLLAGRGKEAMQSIVKHGPRVG
                     TAASWQPVFRFLVAQARRD"
     gene            complement(13704..15311)
                     /locus_tag="VIG70_11680"
     CDS             complement(13704..15311)
                     /locus_tag="VIG70_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8251261.1"
                     /translation="MRFAPPASASPSEDLGQRLEQLNLIGASLSAERDTDRLLEGILT
                     AAKSITRADGGTLYRVTDERTLRFEIVRTSSLKYYLGGTSGNPVPFYPIQLYKDGAPN
                     HSMVAAYAALTGKTVNIADAYTADGFDFSGTRAFDAKTGYRSKSFLTVPMRNHEYETI
                     GVLQLINAHDPRSGEIVAFSASDQRLAESLASQAAIALTNRMLISQLEQLFESFINLI
                     NSAIDEKSPYTGGHCQRVPQLTMMLAEAVNETRDGPLSDFNMTEKDRYELKIAGLLHD
                     CGKVTTPVHVVDKATKLETIYDRIHLIDTRFEILKRDLEIQSLKNQIPERDLRNRLRE
                     LEDDRRFLHACNIGSERMRDDDVERVRRIAAYRWREVSGHEADFLSEDEVKNLTIRAG
                     TLTEDERKIINHHIVATIKMLEALPWPKHLTNVPEYAGGHHERMDGKGYPRGLKREQM
                     SVQARCMGIADIFEALTARDRPYKKGKTLSESLEILGRMKLNHHIDPDLFDIFVRRKV
                     YRRYAEMFLDPEQIDEVDESRIPGYKP"
     gene            complement(15359..17740)
                     /locus_tag="VIG70_11685"
     CDS             complement(15359..17740)
                     /locus_tag="VIG70_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY8251262.1"
                     /translation="MSAVLSQHAPAQSDVGARLAFQKQLQAVTNKIHATNNIDEIMLE
                     VSADVCALFNADRLTIYSVGEDKQSIVSKVKTGLNSFKDLKLPIAEHSIAGYVALAKK
                     TANIKDVYDENELRSLNANLRFLQEVDKRTGYRTKQMLVAPILEAGSNELIGVIQIIN
                     NKAGVPFGSLTEEGVGELSQTLAIAFKQRQKPQVAKTKYDYLIADAVLSAGEFELAAR
                     QARKKAVDIEQVLTDEFQVKIPAIGAALSKFFGVPYEPFKSDRVKPADLLKNLKREYV
                     EANQWVPIDESKDGLAVLCLDPERIRSSRIASNVFPKARINYRVATQKDFRETVNQFY
                     GSEGGGGADLGNVEDLLGAMGDESATDVLDAGAADEASAAADNELVKLVNKVIIDAYN
                     QGASDIHIEPYPGKAKTEIRLRKDGSLGAYIEVPASYRAAIVARIKIMCDLDISEKRK
                     PQDGKIKFKKFGPLDIELRVATIPTQGGVEDIVMRILAAGEPIPLEKMGFTDRNLEQL
                     KNTVTKPYGLFFCCGPTGSGKTTTLHSVLKSLNTPDTKIWTAEDPVEITQKGLRQVQV
                     NKKAGLDFAVIMKSFLRADPDIIMVGEMRDKETTSTGIEASLTGHLVFATLHTNSAPE
                     SITRLLDMGMDPFNFSDALLGILAQRLAKRLCSCKQTYVPESAELTSFLREYCEELMA
                     TSRFKADPKGAMEAVYKSWVQAYGSDKGQLTFYKPVGCDKCGGSGFKGRCGLHELLVA
                     SDRLKKAIQEHARVAEMLAICLEEGMRTLKQDGMEKCLMGVTHMKEVRAVCVK"
     gene            complement(17805..18848)
                     /locus_tag="VIG70_11690"
     CDS             complement(17805..18848)
                     /locus_tag="VIG70_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8251263.1"
                     /translation="MESPLRATGEQLSRLAPLAGLSPERLAELAQATLIERVVRGADP
                     LAGRTDGEQSVFLLAGEILLRFQGGGTLVVVGGSEETRQALNRRKERIARARAITDVD
                     LLVVDDELLDILATWDQVAAARGRDVIASGPFPLSSLRAGVFAPLPTARIDELLRCFE
                     RVKTARGETVIREGDEGDYYYVIESGRFQVGRLVGGAEVVLAELKSGDGFGEEALVSE
                     AKRNATVTALTDGQLLRLNRAHFNELLREPLLRRIDHAEASEKVRRGALWLDVRYPSE
                     YQYDKLPGAINVPLAEVRNMFAVLDRTKEYVAYCQSGRRSAAAAFLFAQRGFKVWLLE
                     GGLKAVLEKPEIR"
     gene            complement(18858..19625)
                     /locus_tag="VIG70_11695"
     CDS             complement(18858..19625)
                     /locus_tag="VIG70_11695"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEY8251264.1"
                     /translation="MRLRVLGCSGGIGGRHLRTTSFLLDGDILIDAGTGVGDLTLAEL
                     SQIDHIFITHSHLDHVTSIPFLVDTVGGMRSRPLNVYATSATIEILKNHLFNWAIWPD
                     FTEIPTPEAPFMRYREIEVGSTVTLAGRSITPIPARHTVPAVGYHLDSGAASLVFTGD
                     TGQNDALWRVVNRIANLKFLIIETAFSNKERQLAEISRHLCPATLAEELAKLERNPEI
                     YITHLKPGEIEPTMLEIEECAGAFRPRMLQNNLVLEF"
     gene            complement(19640..21853)
                     /locus_tag="VIG70_11700"
     CDS             complement(19640..21853)
                     /locus_tag="VIG70_11700"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8251265.1"
                     /translation="MRIALGLAITLLLIGHALQWYPTADGRLRFVTQLEHILYDTRLR
                     MTMPRGRDERIVILDIDEKSLGEVGRWPWSRNLMADLTAKLFDRYGVALVGFDVVWAE
                     RDPSSGIDVLDGLARGELQGNREFQRSYSRLRAGLDFDATFAESLKGRPVVLGYYFNI
                     EDRAVRANSLPEPSLPRGTFAGRNVALFGWRGYTGNLPIYLQNAAGAGHFNPLPDFDG
                     VSRRVPMLIEFDGAYYEALSLALVRAWMALSTGQAVRVAPGYPEQRPNDLEWLDVGGV
                     AIPVDENAASLIPYRGEKGSFPYFSLADVLRERVAPERLKGRIALVGTTAPALQDLRS
                     TPVGGAFPGVEIHANLIAGILDRELKRRPWYTTGAEVVLLVVGGLVLSVLIPKLSALW
                     ATFATALGMGLIVAFNVAAWSAGGLVLPLASSLLVAGAIYTMNMAYGYFVESRSKRRL
                     AGRFGEYVPPELVDRMARDPERYTMEPKNAELTILFCDVRGFTGISESLDPEALREYI
                     NEYLSEMSAIIRDRHRGTLDKYIGDAIMAFWGAPVDDPQHARHAVLAALEMQRQCGAL
                     NARLAARGWPRLEIGIGVNSGTVRVGDMGSRVRRAYTAMGDAVNVASRLEGRTKNYAV
                     GILVGEATRSRVKDVVFREIDRVRVKGKDEAITVYEPVGERPEEGLREELQSWQEALR
                     AYRAREWDAAETSLLSLQRANPSRGLYAAYLTKIQDLRRRALPPDWDGVTAFDEK"
     gene            complement(21869..22750)
                     /locus_tag="VIG70_11705"
     CDS             complement(21869..22750)
                     /locus_tag="VIG70_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8251266.1"
                     /translation="MAKLILSMDGLVLKEIPLTKERTTIGRKPHNDIQIDNLAVSGEH
                     AVIVTILNDSFLEDLGSTNGTVVNGNPIKKHFLQNNDVVELGKYKLKFIGESAPATTG
                     GGEKADFEKTMVLRPSQMKAAADQVRAPSAAPGGGAQAAVAQRAAAVQQVVATATAAG
                     VADKEAVKPAPIPPPAAAPAAAPAAAAPAAAPAPAAAPAAAPKPGQPLGAIQLLSGAN
                     AGKELELTKPLTTLGKPGVQVAVLTRRPQGYFITHVEGAARPMVNGQAIGAAPHALKD
                     HDVIELAGVKMEFFMKV"
     gene            complement(22770..23594)
                     /locus_tag="VIG70_11710"
     CDS             complement(22770..23594)
                     /locus_tag="VIG70_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEY8251267.1"
                     /translation="MDLSQNLEIATCTDPGMVRSHNEDSIAADTGNGLVVLADGMGGY
                     NAGEVASGMATTVIVSEMRQILASTKPYDVDSRTSEEIAARLVREQVLKANSSIYQAA
                     QSQPQYAGMGTTLVVCLYYDNRMLVAHLGDSRLYMLRDGKFTQVTRDHSLLQEQIDSG
                     IITPEQAKKAQHKNLVTKALGIDPTVEPEIHEYTTRAGDIYLLCSDGLCDMVEDEDIG
                     MTLQTLGGNLKLAAQQLVQMANDNGGRDNVSVILVRVLREYPGRRGVMGKVFGWLK"
     gene            complement(23700..24131)
                     /locus_tag="VIG70_11715"
     CDS             complement(23700..24131)
                     /locus_tag="VIG70_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251268.1"
                     /translation="MGVKAMIEAADLVLAGKHKEVVQDESQATYEGWCRKAEARINWA
                     NHVDFVYNLVRGCNPAPGAWSTLEGKELQVFDARKHPVRTFGAVRGKIGEVVEVGDKS
                     FQVTAQGGRIEVLRAKLGDGKKVAAAELVSAGAIKAGAILS"
     gene            complement(24120..24359)
                     /locus_tag="VIG70_11720"
                     /pseudo
     CDS             complement(24120..24359)
                     /locus_tag="VIG70_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
     gene            complement(24378..24629)
                     /locus_tag="VIG70_11725"
     CDS             complement(24378..24629)
                     /locus_tag="VIG70_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251269.1"
                     /translation="MKIAIVGQQDFGKSVLEAFLARKDEVAGVFCAPEKPGARPDALK
                     VAAQEKGVKVFQFASLKSAEAKQALKNLNVDLGIMAFVL"
     gene            complement(24629..25900)
                     /gene="frc"
                     /locus_tag="VIG70_11730"
     CDS             complement(24629..25900)
                     /gene="frc"
                     /locus_tag="VIG70_11730"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEY8251270.1"
                     /translation="MEALKGVRILDMTHVQAGPTCSQLLAWLGADVIKFENPQGDATR
                     GQLRDVPNADSLYFTMLNCNKRSITVNMKTEEGKQVFVDLLKKSHIIMENFGPGVLDR
                     FGFPWEKIHQLNPAIVMGSIKGFGSSGPYSDFKAYENVAQAMGGAMSTTGVPDGPPFV
                     TGAQIGDSGTGLHLAIGLLAALRQAEKTGQGQYVEVAMMDGVMNLCRVKFRDHQRLTR
                     GDLSEFSVPTYKGMGDVPRAGNDSGGGQLGNAIHCKPHGPNDWLYVVVQEAVWDDLAK
                     RIGPDVGMPEMACDPRFAKISDRRKNQNLMWTLINKFAEKYTKRELMAILNPLDVPCG
                     PIMSTEDLATDEHVRGRDMWVELDHPQRGKWWNVGMPIKLSDSPAQIRRSPTLGEHTD
                     EILQQVLGYEKDKIAKLKSAGAFSVPPKKAA"
     gene            complement(25900..>27375)
                     /gene="oxc"
                     /locus_tag="VIG70_11735"
     CDS             complement(25900..>27375)
                     /gene="oxc"
                     /locus_tag="VIG70_11735"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEY8251271.1"
                     /translation="GFLNGLNALAHATVNCFPMILISGSSEREIVDLQQGDYEEMDQL
                     AIAKPLAKAAFRVLHAEDIGVGVARAIRAAVSGRPGGVYLDLPAKLFAQSIDSSLGKN
                     SLIKVVDAAPKQIPAPDAVRRALDLLKGAKKPLIVLGKGAAYAQVDAEIRALVEKTGI
                     PYLPMSMAKGLLPDTHEQCASAARSFVLPAADVVMLIGARLNWLLSHGKGKTWGGKGH
                     KDWGGQKFIQVDISPQEADSNVRIDAPLVGDIGSCVSALLAGMGSNWARPPAEWLNAI
                     TEKKTTNVAKMAETLAKNPSPMNFHSALNVVRDIVKANPDAMFVNEGANALDFTRSIV
                     DMYKPRKRIDVGTWGIMGVGMGYCVAAAAVTKGQVIAIEGDSAFGFSGMEVETICRYN
                     MPVCIVIMNNNGVYKGTDVDPNGRDPAPTVFVKGARYDKLMEAFGGVGVNATTPAELR
                     KAMEDAIRSRKPTLINAVIDETAGTESGRITSLNPTAGKKK"
BASE COUNT         4546 a         9133 c         9383 g         4313 t
ORIGIN      
        1 ggtcagcgcc cacgccacac cgatcccgac cgccacgacc ggcgccgatt ccttcgtgcc
       61 caaggccgcg accagcgcag cgaagcccag tgccttcgcc cagcgccgat ccgaggcgaa
      121 gccgacgaac gcccacagga acagcggcag catgaacact tcgggatgga agtcgaaggc
      181 gttcgcgttc ctcgtcggca gataggccca gtagagccac ggcagagccg cgggcgccca
      241 atggggttgg ccgagcggcc cgggaccgaa gcgcgcgcgc gcgaggtagt acagcgccgg
      301 cccgcccgct gccaggccca gcgcctggac gaaaaccagc gtctccggcc ggggaacgag
      361 ccagaacacc ggcgcgagca gccagtaggt gggcgactgg tggtcggcaa agaggttgat
      421 cccgcccttc accgccgaca cgtagccgtt gccgtgagtg aggttccaca tcacgttggt
      481 gaagatgccg aggtcgtagc catggctgtc gagcgcccag tggcgcagga gcgacacgca
      541 gaaccaggct aaggccacga cggcggtgac gacccacagg gggcgcgggc ccgcgcgctc
      601 gagcgcaccg agccaggcgc gcccgagctt tattcccagc cggacgaata ggctcttctc
      661 ccacggctcg ctcgagtaac gccggatcgc cgcggcgacc agcgccacga ccaggaccgc
      721 gctcgggccg tgcgtcaggt gccagatcgc gcgcgtagag acatcgcggt ggttctcgaa
      781 cagccacccg aggagcgcag ccgacgcagc cacggcggcg ctgctccaga gcctcgctcg
      841 ccaggagacg cgccgccatc gcagcaccgc ctcgaccacc agggcgacga tcagcagcgg
      901 caggccgtaa tccggcagct ccatgctcgt cccttcgaac gagccccatt ccgtcaccca
      961 cggaaacacg atcagcgtgg cgatgaagat gtctaggagg cgcacaggta gaacgacgag
     1021 gcgtagcggc gcgccagggg aagattgagc gccaggctgt cgagcgcctc gagccctggc
     1081 gcgagaaagc gcggcgtgcc gggcgcgagc caggcgagcg ggccgaggaa gtaatcgcta
     1141 tagcccgtcc tcacctcgag gaaaccggct tcgcccgcca tggcggcgag cgcccgcgca
     1201 tcgagctcgc gctcgtcggg gctgtggtag cgctcgtagg tcttgcgcac caggccacgg
     1261 aacaggccga ccaggcggcg gcggctcggg tcgctcgagt agaagaggcc cccgggccgc
     1321 agcgcccggc gcgccgcccg cagcgtgtcg cggatcgccg catcgtcgag gtgatgcagg
     1381 aaagcgaacg ccgcgacgat atcgaagtcg ccttgccatt ttcccgcgac ctcgaaagcg
     1441 aggtttgcaa actgcgccga cttggcgcgc gcctgggcga tcgccaccgg agagatgtcg
     1501 acgccaagga ccgtccccac gtggggcgcg agccgccgct cgatcgagcc gtcgccgcag
     1561 ccgaggctga gaatccggtg ggcgcggccg gcacgcgcaa tccgcaggaa ctggcgcgcc
     1621 gcccgatcgt gcacccggcg gaacaacggg gtatcgaaaa gcgccgcttc cgcctcgtag
     1681 tgccggtcgt gatagtcgcg ctcgctcatc ggcaaggcgc gagatcgacg atctcgccgg
     1741 cccggtagcg attcagcacc acctgagcgc cgatggtctc catgcgggca tcaaggtcga
     1801 cgtagtggcg tgcccagccg ccgaggccga acggctcggt gaccgcggtc tgcccacgga
     1861 acaggcgcgg gcagccttcc tcgatgcggt cgaagagcgc cgacgcccgg cgctcctgct
     1921 ccagcagcgc tgagtcgatc ggcgccgggc gcagcgcgca gctcgctacg gaaagcggct
     1981 cgctcgacgg cggcgtgcgg ctgatccagt tcgcgccgcg cgagagcgcg tccgcgtcgt
     2041 cgcgccgcca ttcgatcgcg aagcagtcgg cgctgtcgag acgatagccg atccagctca
     2101 gcgtccggcc gtcgaggtgc tcgagggcgc ggccgaggac gcgcacgcgg ccccggtggc
     2161 gctcggcgag cgcggcgagc cgcggcgaat cgggcgcgag ggtgcgctgg ccgcgcacgt
     2221 tgacgaagga cgaatcggga tggacgaacg gcgcgaccac cgccatcggc agcgtctcga
     2281 gcgtgaggta gagcgcgggc tcgcgcagcg cctgcggcgg cacgtcgtag ggaaaccatt
     2341 gcttcgacca cggctcggcg atgaaccagc gcggcggcgc cgcgacgatc gcggtgacga
     2401 gctggagcgc gagcagcacg cccagaccga tgcgcgcgat gcgaagcggc acgaggcgct
     2461 cggcgaggta cgcaaggcag accccggcca gcagaagcag cgccatgccg tagcgggcgt
     2521 tgcccgaggt cgcgagccac gccacgtacg ccagcgcgaa gaagccgagc aggcgccaat
     2581 ccccgacgct ttttcggtaa gcgggcagcg cgagcgcgag gacggcgagt gccgcaaacc
     2641 gcaggtcggg cgcgaatgtc tcggcgtaga gcgagcggtc gagcatgacc atgcggaacg
     2701 gaaggatgag cgccgagacc agatccgggg gaccaaaacg tccgctgcct gcgctcaatc
     2761 ccggatacag cggattgccg aactcgcgca gcatcagcgc gagccaggga ccggcgaaaa
     2821 gcgccaccgc cagcgcgccc ccggctacgt aggccgccgc cgcccggcgg cccgacaaaa
     2881 aggccagggg aagcgcggcg agagcgaaga tcgcgttgga atacttcagc gccgcggcca
     2941 ggccgaacaa aagccccgca acgaagggtt gctgtcggtg aagaagcaaa agcaagcccg
     3001 ccaacacgaa cggaaccagc agcggatcga ggaacgaggt gcccacggtg gcccagaaca
     3061 ccgcgctcgc ggcgccgagc gcggccgcga ggcacgacag gacgatgcgg tcgcgccgcg
     3121 gcaggtgggc aaacagaccc caggcgatcg cgtacagcag cgagatgctc aggccgtgcg
     3181 ccaccgcgag cgccgccgaa acaacgacgc tgtgccagcc cgaagacacc atcaggtaga
     3241 acggcaggta gccgatcggg ttcaggtagc tctgcgcgct ggcggcgaaa aaatcctgct
     3301 gcaggcgccc ctcgagcagc tggaacgggg cgtagtagtg gtagttgagc aggtcccagt
     3361 tgaggtcctt gccggcaacg accgtccacg cggcgcagat cgccgccgga aggcaaagca
     3421 acggcaatag cgccctcaac gtcccttgcg cttgagctgg ctcacgcggt tctgcagatc
     3481 ctgcaacgcg gcctgattgc ccgtgtcgcg gtaaatcgtc tccagcggat ggatcgcgcc
     3541 gctcttgggc gagggccaaa gcgaaaacgc ggtctcgagg tcccggatgg cgccgggccc
     3601 gtcctgcgcg ttggcgcgca ggatcccgcg cagcaggaag tagcccgcca cgtgctgctt
     3661 gggcgcgtcg gaaggcagca acgcgcccgc ccggtcgaga taggcggcaa ccgcgccggc
     3721 ggaatagcct cgcggattgt tcagcaggcc gtcggcgagc ccgagcgcgc cgtagaagtc
     3781 ctcgcccagc agctcgagat ggcggcgcgt gcgcgcgtct tcgggggcct gccccggggt
     3841 cagcgacatc ccgagcacgc tgccgtagcg gcgccgcaac tcgctctgga agtaggcgat
     3901 ccaggcattg ccgatgctct ccttcgcgat cgcatcctcg aagaagcgca cggcctcctc
     3961 ggggaggtcg accgtgatcc gcttcgaatc ggtggaggac ttgtcgacct cgatgtacag
     4021 ccatcgatcg cgccgcgcat tgccggtgta ggcgtcgagc gtgaacacca cgggaacggt
     4081 ctgctcctcg atgaactcgc gcaggatgcg gtccgcgatc tgctggtcgg tgcgcaaggg
     4141 atgaaacagg cgattgccga ggatcaggcc ctgcgcctgg tacagcgtga tgtccggccg
     4201 catctcctcc accatgtgga aatacgccat cggcagcagg tctgcgtccc cctgtcccca
     4261 tacgacggcg ttgggcggca gcagcttgag gacggtctgg gcatagcgcg cggcccactc
     4321 gtggttggcg cgcaggttca catgggcgcc gaccgcggca acgagcgcga gcagcgcggc
     4381 gcccgcagcg agcgcgcgcg cacgcggcag cgcgtagcgc tcgacgagcc agccgaagcc
     4441 caggccgagc cacagtgcgc cgatggcgta cgcgggaagc gggtagacgt ggaaaatgtg
     4501 cttgcggaag acgtcgtaat cgaatccgag cagcagcacc agcacgaccg acggcatcag
     4561 gaaacagatc gtgaggaacg cggcgatccg ccggccaagc gtgcgccact gcagcgcaaa
     4621 cccggccgcg gcgaccacgg tgccgatgac ggcgaattgc acgagcagct cccggcccac
     4681 aaacgtgagg tatctcacct tgtcgagcca gcctgcggtg ggcgaatgat ccacttcggc
     4741 atagcccgcc cggctgagga agaacaggat ctcggtgacg ctgtcgagct cgccgtagaa
     4801 gctgatcggc aggccggtcc acgatcggcg caccagccag gcgtagggca gcaggccgac
     4861 gaccaccagg atcaacagga gcccgatgcg ccgcaggagc tcggcgcgca gcggccagag
     4921 cagcaccgcg aaggccggcg cggcgagcag catcagcgga tagtggttcg cgaggctcag
     4981 gccgaacacc agcgccatcc acggcagtac gccggggcgc cgttccgcgt ccagcccgag
     5041 gtacacgagc agcaggagga agaacgtgtt cagcgtgtac acctcggcga tgatcgattg
     5101 cgaccagaac accggcgaca ggccgagcgc gagcgccgcg acgtaggcgg gcagcacccc
     5161 cgggatcagg cggcgcatgc agagccaggc cgcgccggcg ctgagcgcgc cgaacagcgc
     5221 gctcgccagg tggacgcggt aggcgaccga gcctaccggc aggtaggtga agagatggcc
     5281 gatcagcgtg aacagcggat acccgggcgg atgctcgatg cccaggaagt agcaggacag
     5341 gacgaacagc gagtcgtcct cgaggctcac ggtgcgcggc gtggtgaccg cataaagcgc
     5401 gaacagcgcc agcgcgactg cacccgcctg catccagtct gaacgcgcgg gccggaacgt
     5461 agtgggggaa agcgtcaagg cgggcgggaa ggtcaaggct tgcgggcgtt cgatccgccc
     5521 caggtgagcg acgcggtggt atagaagttc cagagcgccg cgatcagcgc gccggcgacg
     5581 ccggcggccc agtatgcgac cgactgcagg aacagccact ccgctaccgc gaggttgatg
     5641 aacgcgccga tgccgcaggc gacgtagaag ctcagcaggc cgcgccacag gcgcctgccg
     5701 cgcaagcgct ggtcgccgta ggtgaacatg ttgttcaaga agaaattgct ggtcatcgcg
     5761 acccaggtcg ccagcgcctg gctggcgagg aagttgcccc cggtcgcctg gaacgcggcg
     5821 gtcagcaccg cgaggtgcac gccgacgccg gtcacgccca cggcgccgaa caggaagaag
     5881 cgcgccggca ggaagcggcc ggtcatgtgg tagagcagca gcatgaaata ctcggcgatg
     5941 acgcgcgccc cgagcttgct ttcgccgtgc tggcggcttc gcatgcggta cggcagctcg
     6001 gcgacgcgca cctcgccgcg cgccgccgcc accaggtcga gcaggatctt gaagccgcgg
     6061 ccatagagcc ggcgcaccac gcgctcgagg aagctgcgcc gcacgaggaa gaatccgctc
     6121 atcgggtcgg tgaggtcctt gcacaggagc tggctgagcg cgctcgcgag ccggctgatg
     6181 cgcacgcgct cggccgcgag ctcgccggtg ctgccgcctt gcatgtagcg gctcgccacg
     6241 acgacgtccg cgccctcgcg cttcaccgcc tcaagcagct tgggcaccag ggtctcgtcg
     6301 tgctgcaggt cagcgtcgat gaccgcgacg tagggcgccg agctcgcgag catgccctcg
     6361 atgcaggccg aggccaggcc gcgccggccg atgcggtgga ggcagcgcac gcgccggtct
     6421 tcctgcgcgc ggccgcgcac cacgtcctcg gtgccgtcga aggcgtcgtc gaccacgatg
     6481 atcgtttccc agtcgagccc cgccagcgcc cgctcgagcg cgccgagcag cggcgcgatg
     6541 ttgtcgcgct cgccgaatgc cggaaccacc acggtgagct caggcaatgc gccctccccg
     6601 cttccatgca aaccaagccg ccgccagcca gcctatcgaa gcaaagaccg atatcgcggc
     6661 ccccagccgg aattcccaag gggtgtactc cagccgcagc cggtgccggc cccggtcgag
     6721 cgccacggcg cgcagggcat agttggccgg catgatctcg tagcggcggg tatcgccggc
     6781 gtcgagcggc accgcgcgcc aggccggcgt ccacgcatcg gtcacgagca gcaccgacgg
     6841 ccgctcgagc tccgcctcga tttccatgaa atcgctccct tcgcgcagga ttcttgcacg
     6901 gccgggcgtt tcgcccgcga ccggcaccgg cgcgggctcg cgctcgagga tcacctccct
     6961 cgacgggtcg aagcccggcg agcccatggc ctgcaggatc gccgcgcgcc cggcacgtac
     7021 ctggtgcgag ccgatgagct ccagccggcc gagcggcggc gtgccgccgg ggtggatggt
     7081 catgaccccg tcgtcgaacg cgaccacgta cttcactcgc accatggcga gcaacgggtg
     7141 gaactgccgg aagatgacgt gcggaatgtt gtttttctcg ggatcttcgc ccgcgctcca
     7201 gctgatgaac tcggcgtagc gccgcgtaac gccgggatcg tacccccagg cgtcgaacgc
     7261 tcccatcgag atcgccgcgt tcgggcgcgc gagattcagg atacggtatt cgcccgggtt
     7321 gcggcgcaga aagccggcga gctcggggat gaatatgtgc gtgctgtcga acgtcgggcg
     7381 gttgagccgc gcgtacgcga aaacctccgc caccgcgagg acgccgagca ccacggcggc
     7441 gcgcgcttcc ttgcgcgccc agagcagcag tccggccgcg gcggcgagcg tgagcgcggc
     7501 gaccatcagt ccgagcgcgg cgaagctccg ggaggcgaac aggaacccgg catcggcata
     7561 gcgcgcatgg tcgaggtagg tctgccccgt cgccagcacc gcgccggcca ccggccgcca
     7621 gtccacgctt tgggtgagga ccccggcgac gcccagcgcc agcgcaccgg ctcccgcggc
     7681 ccacacggcg cctcgcggca cgtcgcggcc gcgcaatagc cggtcgagcc cgctccccgc
     7741 gaacaggatg aggatcagcg cggtgatgaa gatgaacttg ccggcgccgc gaaaacgatc
     7801 gaaccacggc agccagtcga acagcagcgg gaagagcggg ctgttgtcgc ccagcgcgag
     7861 gactgccgcc acgccggcgg tgaccagcag gggaacccgg ttcgcaagct gtccccgggc
     7921 gatgccgtaa gccgccagcg cgaggcccgc gacgccaatg aaggccgacg cttcccacag
     7981 gtaccagcgg ccccagtacg gatgtgtggc gacgtcgccg aagaatcccg gcgccagcag
     8041 cgtgaggaaa ttctcggtcg gaaagctgaa gtacttcgcc agctcgagcg acatcgggcg
     8101 gtcgcggacg gtttcggcgg tcgcctggaa accggtgagg agctgcaccg cggccaggct
     8161 tgcgcccccg gcgtagaggc tggcgacacc cagaatctgc atcctgagca cgctgtagag
     8221 gccggccgtc aacgccgtca ggtagacggt ctgcggatgc ccggcgaaga tctgcatcgc
     8281 caccgccgcc atgcccgcga gcagccagcg cggccggcgc gagtcgatcc acgcgtcgag
     8341 cgcgaggaac acgagcggca cccacgccat cgccgccatg cccgcaatgt ggccggcgta
     8401 gacccgcaag aagtgcggcg cgcagaacat ggcgagcgcc gcggcgacga acgcggcaaa
     8461 aggctgcagc ccgcggcgca gcgcccagag atacatgaac gcaccgagca gccacacgtt
     8521 gagcgcgacg ctccagttgg cggcgagcgg cagcggaagc gcgagaaaca gccagttggg
     8581 cgggtagagc agcgccgatt gcatgccgcc gaagaacggc acgccgccgt agatatgcgg
     8641 gttccacagc gcaaggttgc cgcgcgcgag ctcgccgaag ccgaagttgc gccagtgcag
     8701 aaagtgcagc gcgaggtcgg tattgtcgac gccgagcacc cgcggacccg gtgcgatgag
     8761 gacgtcgaag aacatcgcga acacgagcgc ggcgaggccg ccgagacccg cagggagcgg
     8821 tctcacccgt cggtcttgag acgaagaacg ctgagcagga acgaggcgag catcagctcc
     8881 atgccgccga caagcagcgc ggtggccggg atcgtgtcgc gcatcaccac gcgcggatcg
     8941 agaggcccga agccggtctc gcgccactgc cagaaggccg cggcgctcca cgcaagaccg
     9001 cccagcgcca tgaagaggcc cgccagcagc cccctctcca gggtcaatac cttcagcagc
     9061 cggtcgagtc ccggctggcg cggcagcagc ccggcattct gcgccgagac gcgggcgaag
     9121 agcgcgaaca ggcagagctg cagaccgagc agcgcggcgg ccgaggcgta cagcatgctg
     9181 tggatgtcga gcccggcgcc gaggatgtgc agcggcgtga agtagagcgc gctcatcagg
     9241 ccgatgccga gggcgagcaa ggccaacccc ggatacagga atagccagcg cgggctgaag
     9301 agcagcagga acctcaggtg ccgccagccg tcgcgccagc gccgcaggtg cgccggccga
     9361 ccgcggccgt cggggtgcag cgtggtggcg acctcggcga tgcgccatcc cgcgagcgcc
     9421 gccttgacca ccagctcgct cgcgaactcc attccggggg tcctgaggtc gagcgaccgg
     9481 accgcctcgc ggtcgaagcc gcgcaggccg cagtggaagt cgccgacgcg cgcgccgaag
     9541 aacaggcgcc cgacgaagct gaggaggggg ttgccgaggt agcggtgcga gggcggcatt
     9601 gcgccgggca ggatgccccc ccggaagcgg tttcccatga ccagcggata gccctggcgc
     9661 agcttttcca cgaaaagctc gaggcggccg aagtcgtagc tgtcgtcagc gtcgcccatg
     9721 atgacgtagc gcccgcgcgc ggcggcgatc cccgccgcca gcgccgcgcc gtagccgcgc
     9781 tcggcgactt ccactacgcg agctcccgcc tgctgcgcga gtgccgccga gccgtcatcg
     9841 gagccgttgt cggcgaccag cacctcgcca gcgattcgcg cgcgctcgag gaagccgcgc
     9901 gccttggcga tgcagcggcc gaccgtagcc gcctcgttga ggcacggcat gagaatcgtg
     9961 agttcggtca tcctcccgga tcccgcaggc aaaaaaaaag gtaccccgaa gggtaccttt
    10021 gattgaagct aagctttttt attagccgcg gcaggagccg ggcagatact tgccaggcac
    10081 gttcgtgccg tcggtggtgc taccgcagcg ccagccgaac agcgccatgc tgttgcccgg
    10141 ggtaacggtc gccgtagtgt tgggcgagga cagcggcgtc agcgtgacca ctgagccgtt
    10201 caccgacagc gaaactccct gcacggtggc cgagacgttg ccgttcgcgc tcgtgttgac
    10261 ggccgcgacg tacttcgtcg ccgtggtgcc gccgtactcg cagccccagc cgttgggcgc
    10321 aatcaggccg gccgggccgg actgatacac ctcagtgatc gaggtgcggc atgcgctcat
    10381 gcccagaatc acttcagaca tcttcgcgcg gatcgtgtag tcctgatacg ccggcagcgc
    10441 aactgccgcc agaatgccga tgatcgcgac aacgatcatc agttcgatca gggtgaagcc
    10501 cttctgcagc tgtttcatgg aactcccctt cctgtttgat aaatgtagcg gtcggcttta
    10561 taccggacgc gccctctagg tgcaacgtcc gtgccatggc ccgacacccc ccgaatggcg
    10621 ctttttgcgg gaaaaaatca cgccgcgcgc ctttcggcag acaggatccg tcagccccct
    10681 gcccattttc gtcaatcccg ccgggcctgg gccacgagga agcggaatac cggctgccat
    10741 gaggctgcgg tgccgacgcg gggaccgtgt ttcacgatcg attgcatggc ttcctttccc
    10801 cgaccggcga gcagcagccc ggccatgtgg gcggccagcg cgtaagcggc gaactccgcg
    10861 ggcaggctag cgtcggcttc caggatctgc ccggccgccg aagcgatttc tccgccgttc
    10921 tcggcgccga gcgccgcatg cagccgcagc cactgccgtt cggtttccgt caggcgcgtg
    10981 cggcaaggcg agccggccag cagccgccac accgcctgcc gctcggcgcg ccccaagtgc
    11041 tgattcacca gccaggcagc atcgagcagg acgcgccgca gggtcgatgc cggaacgcgc
    11101 acgctgcaat cgacgagcgc cgtgcgcagc agcacgaggt ctcccgagac cgccgccgac
    11161 agcgactcga gctcggcgag cctgccgctc agcaggtaag cgccggcggc gcgagcctgg
    11221 gcggcgtacc cggcccggac cagccacggc cggctcgcgg gtgaaagtcg cgccggatcg
    11281 ggctggccga cgcgcggccg gtcgaacaga tcgagcagcg gaaagcccgc ctcgagcagc
    11341 cgcgggattt cttcgtccgc cgggcggtgc aggaagcgcg cctttgcggc gttgaggtcg
    11401 agcacggggt agaagtcgga gttcgccggc acgcggaagg aggcgaagta agggtgcatc
    11461 gcggaacggc cggcgacgcg gtggagcagc aggtcgtcca agctgcggat cccgaaacgc
    11521 tcgagatcgg cgcgcaggcg cgggctgtcg aacgcgcgcg cgtcgggggc gcgcagcgtg
    11581 cctttgtgga cggcgaccac cagcaggtcg cccgcgttgg gcatccagat ctcgtagtcg
    11641 gagaagtggg catcgagcgc cgcgatgatg gtggcgagca gcacgggcga gagctcgtag
    11701 acgttcaccc attgcaccag caggccgccg tcgcgcaggt gccggcgcac atcgcggtag
    11761 aactccgacg agaacaggct cgcgacgccg ctcacccagg ggttggaagg ctccgaaacg
    11821 atcacgtcgt agcgttcccc gcgcgccgag aaataggtct tcgcgtcctc gaaatgcagc
    11881 cggctgcgcg ggtcgtcgaa cgcgcgcgcg ttgaacgggc gcagcgctgc cgccgccgtg
    11941 accaccgccg gctcgatctc gatggtatcc accgactcga cgcggtcgga gccgagcatg
    12001 acgtgggtgg tgagcccggt gccgaagccg atgttcgcga tgcggcgcgc ctccggcagg
    12061 agaaagaacg gcagcgcgcc ggtcagcgtc atcgtgatct cgtcgtgcgt cacgccggcg
    12121 ccgtccaccc ggatggcgcc ttcggacttg ccgttggtgc gcagcgcgat cagctccttc
    12181 cccccggtga tgctcacgct cgcggtcttc ccgtcgaggt gcagcctcac ttcttcccca
    12241 ccgccctggc gcatcagcac attggagcgg tacacgcccg aggccatgtg caggggatcg
    12301 agccgcacgc cgatgatcgc gaagcacacg gcggccgccg acaccgccgc cgcggcatag
    12361 ggccggcggc cgacccccag aagcaccact ccgagtgcga ggtcgatggc tgcgcccgcg
    12421 atgatgagcc ccttcagccc cagcaacgga agcccgaggt gcaccgcggc gaccacgccg
    12481 gcgatcgcgc cggcggtatt cgcggcgtac acctggccga tcgcgcgctc gcccgcgccg
    12541 gcccgcatca gggaggcggt gaggagcggc agcgtcatgc cggcgcagaa cgcggccggg
    12601 aacatcacgg cgaggcagat gccatggctc accgcgttga gagcgaggta cccccccggg
    12661 gtgcgggcaa gcgattgcat cgtgagctgc atgaactcga agctcgccac gtagaccggc
    12721 agcgtcgcga gcgcggcgat ccccatcgcg acctgcacca ggcccagcag gcgcagcgtg
    12781 tccctggccg catcgatcct gcgccgcacc cacaggccgc cgaaggcaag gcccaggatg
    12841 aaggccgaca gcatcagctc gaaggcatgc gtcgaggtcc cgaggaccag cgcaagcatg
    12901 cggatccagc cgatctcgta catgaacgac gacatgccgg tcagcgccgc gaccgcgagc
    12961 agcaggcggc ccatggggac cggcgcgttt tccgccgcca cggaaggcgc cggcgcggtg
    13021 tcggatcggc gcggcagaaa catgaccccg gcggcgaccg cgaggttcag cacacccgcg
    13081 atggtgagcg tgccgggcag gccggccagc tcgatgaagt agaacccgct cgcgagcacg
    13141 cccgcgccag cgcccaggct gttggtgaag tagagcatcg cgattgcata ccccgcgcgc
    13201 tcgggccgga ggcgcagcac gccgccggtc atcaggggaa acgtcatgcc gagcagcacc
    13261 gattgcggca ggatgagcgc cgcggccagg gtccatttaa gggcgtgcac cgcggcgggg
    13321 gagccgagcg caggaatcac ggcttcgaag gcgaacgcgg tggtggcgac gaacaccgag
    13381 tggaacacga gcgagatcag accgatcagc gcctcgatga ccgcgtaggc gaggagcagg
    13441 tctcgccagc gcgcggagat gcggctcgcc ccccaggcgc cgagcgccat gccgcccatg
    13501 aagatcgcga gcaccagcgt ctgggcgtag gcagcgtggc cgaggaagag cttcaggtag
    13561 tggctccaga tcgactcgta gatcaggccg gcgaagcccg agacggtgaa caggacgaac
    13621 gccgccggca gcaggatcag cgggcgaacg gtggcgccta gctgcatcgg gagcgcagca
    13681 gggggcgcaa ggttgcgatg tgcctaaggc ttgtagccgg ggatgcgcga ctcatcgacc
    13741 tcgtcgatct gctccgggtc gaggaacatc tcggcgtagc gccggtagac cttgcggcgc
    13801 acgaagatgt cgaacagatc aggatcgatg tgatggttga gcttcatgcg gccgaggatc
    13861 tcgagcgatt ccgagagcgt cttgcctttc ttgtaggggc ggtcgcgcgc ggtgagggcc
    13921 tcgaagatgt cagcgattcc catgcagcgc gcctgcaccg acatctgctc gcgcttcagc
    13981 ccccgggggt agcccttgcc gtccatgcgc tcgtggtggc cgcccgcgta ctcggggacg
    14041 tttgtcaggt gcttcggcca cggcagcgcc tcgagcatct tgatcgtggc gacgatgtgg
    14101 tggttgatga tcttgcgctc gtcctcggtc agcgtgccgg cacggatggt gaggttcttg
    14161 acctcgtcct cggacaggaa atccgcctcg tgccccgaga cctcgcgcca gcggtaggcc
    14221 gcgatgcgtc gcacgcgctc gacgtcgtcg tcgcgcatgc gctcgctgcc gatgttgcag
    14281 gcgtgcagga agcggcggtc gtcctccagc tcgcgcaagc ggttgcgcag atccctttct
    14341 ggaatttgat tcttaaggga ttggatttcc aggtcccgct tcaggatttc gaagcgcgtg
    14401 tcgatgaggt ggatgcggtc gtagatcgtt tcgagcttgg tcgccttgtc gaccacgtgc
    14461 accggcgtgg tcaccttgcc gcaatcgtgc agcagccccg cgatcttcag ctcgtagcgg
    14521 tctttctcgg tcatgttgaa gtccgagagc ggaccgtcgc gcgtctcgtt caccgcctcg
    14581 gcgagcatca tcgtgagctg cggcacgcgc tggcagtggc cgccggtata gggcgacttc
    14641 tcgtcgatcg ccgagttgat caggttgatg aaggactcga acagctgctc gagctggctg
    14701 atcagcatgc ggttggtgag cgcgattgcc gcctgcgagg cgagcgactc ggcgaggcgc
    14761 tggtctgagg cggaaaacgc cacgatctcg ccgctgcgcg ggtcgtgcgc gttgatcagc
    14821 tgcagcacgc cgatggtctc gtactcgtgg ttgcgcatcg gcaccgtgag gaacgatttc
    14881 gagcgatacc cggtcttcgc gtcgaaggcg cgcgtgcccg aaaagtcgaa gccgtcggcg
    14941 gtgtaggcgt cggcgatgtt caccgtcttg ccggtgagcg cggcgtacgc cgcgaccatg
    15001 ctgtggttgg gcgcgccgtc cttgtacagc tggatcgggt agaacggcac cgggttgccg
    15061 ctggtgccgc cgaggtagta cttgagcgag ctggtgcgca cgatctcgaa gcgcagggtg
    15121 cgctcgtcgg tcacgcggta gagcgtgccg ccgtcggcgc gggtaatcga cttggccgcg
    15181 gtaaggatcc cctcgagcag ccggtcggtg tcgcgctcgg ccgaaagcga cgcgccgatc
    15241 aggttgagct gctcgaggcg ctggccgagg tcctccgacg ggctcgccga cgctggcgga
    15301 gcgaagcgca ggcccgccgt ggtgtcgatg atgctcgctg cgccgatgtc gcgcttgctc
    15361 acttgacgca aacggcgcgc acttccttca tgtgcgtaac gcccatcagg catttctcca
    15421 tgccgtcctg cttgagcgtg cgcatcccct cctccaggca gatggcgagc atctcggcga
    15481 cgcgcgcatg ctcctggatc gccttcttca gccggtcgct cgcgaccagg agctcgtgca
    15541 ggccgcagcg ccccttgaag cccgagccgc cgcacttgtc gcagcccacc ggcttgtaga
    15601 aggtgagctg gcccttgtcg ctgccgtagg cctgaaccca gctcttgtac accgcctcca
    15661 tggcgccctt ggggtccgcc ttgaaccgcg acgtggccat cagctcctcg cagtactcgc
    15721 gcaggaacga ggtgagctcg gcagactcag gcacgtaggt ctgcttgcaa ctgcacaggc
    15781 gcttcgccag gcgctgcgcc aggatgccga gcagcgcgtc ggagaagttg aacgggtcca
    15841 tgcccatgtc gagcaggcgc gtgatcgatt ccggcgccga gttggtgtgc agcgtggcga
    15901 acacgaggtg gccggtgagc gaggcctcga tgccggtcga ggtcgtttcc ttgtcgcgca
    15961 tctcgccgac catgatgata tcggggtcgg cgcgcaggaa cgacttcatg atcacggcga
    16021 agtcgaggcc cgccttcttg ttcacttgca cctggcgcag ccctttttgc gtgatctcga
    16081 ccggatcctc cgcggtccag atcttggtat cgggcgtatt cagggacttg aggaccgagt
    16141 gcagcgtcgt ggtcttgccc gagccggtcg gcccgcagca gaagaacagg ccgtagggct
    16201 tggtgaccgt attcttcagc tgctccaggt tccggtcggt gaaccccatt ttctcgagcg
    16261 ggatcggctc gccggcggcg agaatccgca tcacgatgtc ctcgaccccg ccctgggtcg
    16321 ggatcgtcgc gacgcgcagc tcgatgtcga gcgggccgaa cttcttgaac ttgatcttgc
    16381 cgtcctgcgg cttcctcttc tccgagatgt cgaggtcgca catgatcttg atgcgcgcca
    16441 cgatcgccgc gcggtagctc gcgggcacct cgatgtaggc gccgagcgaa ccgtccttgc
    16501 gcagccgaat ttcggtcttg gccttgcccg gatagggctc gatgtggatg tccgaggcgc
    16561 cctggttgta ggcgtcgatg atcaccttgt tcacgagctt cacgagctcg ttgtcggccg
    16621 ccgccgaggc ctcgtcggcg gccccggcgt cgagcacatc ggtcgcggac tcgtcgccca
    16681 tggcgccgag caggtcctcg acgttgccca ggtcggcgcc gccaccgccc tcggagccgt
    16741 agaactggtt caccgtctcc ctgaagtcct tctgcgtcgc gaccctgtag ttgatgcgcg
    16801 ccttcgggaa cacgttggag gcgatgcgcg aggaacggat gcgctcgggg tcgaggcaca
    16861 gcaccgcgag gccgtccttg ctttcgtcga tcggcaccca ctggttcgcc tcgacgtact
    16921 cgcgcttcag gtttttcagc aggtcggcgg gcttgacccg gtcggacttg aacggctcgt
    16981 agggcacgcc gaagaacttg gagagcgccg cgccgatggc aggtatcttc acctgaaatt
    17041 cgtcggtcag cacctgctcg atgtcgaccg ccttcttcct cgcctggcgc gccgcgagct
    17101 cgaactcgcc ggccgagagc acggcgtccg cgatcaggta atcgtacttg gtcttggcga
    17161 cctggggctt ctggcgctgc ttgaacgcaa tggcgagcgt ctgcgagagc tcgccgacgc
    17221 cttcctcggt cagcgagccg aacggcaccc ccgccttgtt gttgatgatc tggatcacgc
    17281 cgatcagctc gttcgagccg gcttccagga tgggcgcgac cagcatctgc ttggtgcggt
    17341 agccggtgcg cttgtccacc tcctgcagga agcgcaggtt ggcgttgagc gagcgcagct
    17401 cgttctcgtc gtacacgtcc ttgatgttgg cggttttctt ggcgagggcg acgtagccgg
    17461 cgatggaatg ctcggcgatc ggcagcttca ggtccttgaa cgagttgagg ccggtcttca
    17521 ccttcgacac gatggactgt ttgtcctcgc ccaccgaata gatggtgagc cggtcggcat
    17581 tgaagagcgc gcacacgtcc gcgctcacct ccaacatgat ctcgtcgatg ttgttggtgg
    17641 cgtggatctt gttggtgacc gcctgcagct gcttctggaa cgcgagacgc gcgccgacgt
    17701 cggactgcgc cggcgcgtgc tggctcagga ctgcgctcat ttgcgacctc ccctgcccct
    17761 gcttatagct tgaccttaaa tgataagcct aggatccctg attttcaacg aatttccggt
    17821 ttttccaaca cagcttttaa ccctccctcc agcaaccaca ccttgaagcc gcgttgagcg
    17881 aacaaaaatg ccgcagccgc gctgcggcgg ccgctctggc agtacgcgac gtactctttt
    17941 gtgcgatcta gcacagcgaa catgttgcgg acttccgcca gcgggacatt gatcgccccg
    18001 ggcagcttgt cgtactggta ttcggagggg tagcgcacgt cgagccagag cgcgccgcgg
    18061 cgcacttttt cggacgcctc ggcatggtcg atgcgccgca gaagcggctc gcgcaggagc
    18121 tcgttgaagt gcgcgcggtt gaggcgcaaa agctggccgt cggtgagcgc cgtgacggtc
    18181 gcgttgcgct tcgcctccga gaccagcgct tcctcgccga acccgtcgcc gctcttgagc
    18241 tcggcgagca ccacctcggc gccgccgacc aggcgtccga cctggaagcg tccggactcg
    18301 atgacgtagt agtagtcgcc ttcgtcgcct tcgcggatca cggtctcgcc gcgtgcggtc
    18361 ttgacgcgct cgaagcatcg caggagctcg tcgatgcgcg ccgtgggcag cggggcgaac
    18421 acgccggcgc gcaggctgga gagcgggaac gggccgctcg caatgacgtc gcggccgcgc
    18481 gcggccgcga cctggtccca ggtggccagg atgtcgagca gctcgtcgtc gaccaccagg
    18541 agatcgacgt cggtgatggc cctggcgcgc gcgatgcgct ccttgcgccg gttgagcgcc
    18601 tggcgcgtct cctcgctgcc gccgaccacc accagcgtgc cgccgccctg gaagcgcaga
    18661 aggatctcgc ccgcgagcag gaacaccgac tgctcgccgt cggtgcgccc ggcgagcgga
    18721 tcggcgccgc gcacgacgcg ctcgatcagc gtcgcttggg cgagctcggc gaggcgctcg
    18781 ggcgagagcc ccgcgagggg cgccaggcgg gaaagctgct cacctgtagc cctgagcgga
    18841 ctctccatgc tcgatgctca gaactccagc acgaggttgt tctgcagcat gcgcggccgg
    18901 aaggcgccgg cgcattcctc gatctcgagc atggtcggct cgatctcgcc gggcttcagg
    18961 tgcgtgatgt agatctcggg gttgcgctcg agcttggcga gctcctcggc gagcgtcgcc
    19021 gggcagaggt ggcgcgagat ctcggcgagc tggcgctcct tgttggagaa cgcggtctcg
    19081 atgatcagga atttgaggtt ggcgatgcgg ttgacgacgc gccacagcgc gtcgttctgg
    19141 ccggtgtcgc cggtgaacac caggctcgcg gcgccgctgt cgaggtggta gccgaccgcc
    19201 ggcacggtat ggcgcgcggg gatcggcgtg atgctgcgcc ccgcgagcgt gaccgtgctc
    19261 ccgacctcga tctcgcggta gcgcatgaac ggcgcctccg gggtgggaat ctcggtgaag
    19321 tcgggccaga tcgcccagtt gaagaggtgg ttcttgagga tctcgatggt ggcgctcgtc
    19381 gcgtacacgt tgagcggcct ggagcgcatg ccgccgacgg tgtcgacgag gaacgggatc
    19441 gaggtgacgt ggtcgaggtg cgaatgcgtg atgaagatgt ggtcgatctg cgacagctcc
    19501 gccagggtca ggtcgccgac cccggtgccg gcgtcgatca ggatgtcgcc gtcgagcaga
    19561 aacgaggtgg tgcgcaggtg gcgcccgccg attcccccag agcagccgag cacgcgtaaa
    19621 cgcatgcgcc gggcctctcc tatttttcgt cgaacgccgt gacgccgtcc cagtccggcg
    19681 gcagcgcccg gcggcgcagg tcttgtattt ttgtcaggta cgccgcgtac aggccgcggc
    19741 tcgggttggc gcgctgcagg ctcaagaggc tcgtctccgc ggcgtcccac tcccgggcgc
    19801 ggtaggcgcg cagggcctcc tgccacgact gcaactcctc gcgcagccct tcctccggcc
    19861 tttctccgac aggctcatag acggtaatag cctcgtcttt gcccttgacc cggacgcggt
    19921 cgatttccct gaataccaca tctttgactc ggctgcgcgt ggcctccccc accaggatgc
    19981 ccacggcgta attttttgtt cggccctcca ggcgcgaggc gacgttcacc gcgtccccca
    20041 tggcggtata ggcgcggcgc acgcgcgagc ccatgtcgcc gacgcggacc gtgccggaat
    20101 tgacgccgat gccgatctcg agccgcggcc agccgcgcgc ggccaggcgc gcgttcagcg
    20161 cgccgcactg gcgctgcatc tcgagcgccg cgagcaccgc gtggcgggcg tgctgcgggt
    20221 cgtccaccgg cgcgccccag aacgccatga tcgcgtcgcc gatgtacttg tcgagcgtgc
    20281 cgcggtggcg gtcgcggatg atcgcgctca tctcgctcag gtattcgttg atgtactcgc
    20341 gcagcgcctc ggggtcgagc gattcggaga tgccggtgaa gccgcgcacg tcgcagaaga
    20401 gaatcgtcag ctcggcgttc ttcggctcca tcgtgtagcg ctccgggtcg cgcgccatgc
    20461 gatcgaccag ctccggcggc acgtactcgc cgaagcgccc ggcgagccgg cgcttggagc
    20521 gcgactcgac gaagtagccg tacgccatgt tcatggtgta gatcgccccg gcgacgagca
    20581 gcgacgaggc gagcggcagc accagcccgc cggcgctcca ggcggccacg ttgaaggcga
    20641 cgatcagccc catgccgagc gcggtggcga aggtcgccca caacgctgac agcttcggga
    20701 tcagcaccga gagcacgagg cccccgacga cgaggagcac cacctcggcg ccggtggtgt
    20761 accagggccg gcgcttgagc tcgcggtcga gaatgccggc gatcaggttg gcgtggatct
    20821 cgacgccggg aaaggcgccg cccaccggtg tcgagcgcaa gtcctgcagc gccggcgcgg
    20881 tggtgccgac gagcgcgatc ctgcccttca gccgctcggg ggcgacgcgc tcgcgcagca
    20941 cgtcggcgag cgagaagtag gggaagctcc ctttctcgcc gcgatagggg atcagcgacg
    21001 ccgcattctc gtccacgggg atcgcgacgc cgccgacatc gagccactcc aggtcgttcg
    21061 gccgctgctc gggatagccc ggcgccacgc gcaccgcctg cccggtgctc agggccatcc
    21121 aggcgcgcac cagggcgagc gacagcgcct cgtagtaagc gccgtcgaac tcgatcagca
    21181 tcggcacgcg acgcgagacg ccgtcgaagt cgggcagcgg gttgaagtgc ccggcgccgg
    21241 ccgcattctg gaggtagatc ggcaggttgc cggtgtatcc gcgccagccg aagagcgcga
    21301 cgttgcgccc ggcaaacgtg ccgcgcggca ggctgggctc gggaagcgag ttcgccctga
    21361 ccgcccgatc ctcgatgttg aagtaatagc cgagcacgac cggccggccc ttcaggctct
    21421 cggcgaaggt cgcatcgaag tcgaggcctg cgcgcaggcg ggaataggac ctttggaact
    21481 cgcgattccc ctgcaactcg ccgcgggcga ggccatcgag cacgtcgatt cccgagctcg
    21541 ggtcgcgctc ggcccacacc acgtcgaagc ccaccagcgc gacgccgtag cggtcgaaga
    21601 gcttcgccgt gagatcggcc atcaagttgc ggctccaggg ccagcgcccg acctcgccga
    21661 ggcttttctc gtcgatgtcc aggatgacga tgcgttcgtc gcgcccgcgc ggcatggtca
    21721 tgcgcaggcg cgtgtcgtac aggatgtgct cgagctgggt gacgaagcgc agccggccgt
    21781 ccgcggtggg ataccactgc agcgcgtgac cgatcaggag aagcgtgatc gcgagaccga
    21841 gcgcgatcct caggagctag acctgccttt ataccttcat gaagaattcc atcttcacgc
    21901 ccgcgagctc gatgacgtcg tgatccttga gcgcgtgggg cgcggcgccg atcgcctggc
    21961 cgttgaccat cggacgggcg gcgccttcga cgtgggtgat gaaatagcct tgcgggcggc
    22021 gcgtgagcac cgcgacctgc acgcccggct tgccgagcgt ggtgagcggc ttggtgagct
    22081 cgagctcctt gccggcgttt gcgcccgaga gcagctggat cgcccccagg ggctggccgg
    22141 gcttgggcgc ggccgccggg gcagccgctg gcgcgggggc tgctgcgggc gctgccgccg
    22201 ccggtgctgc cgcgggagcc gctgccggcg ggggaatcgg agccggtttt accgcttcct
    22261 tgtcggcgac gccggcagcc gtggcggtcg ccaccacctg ctgcaccgcc gccgcgcgct
    22321 gggcgactgc cgcctgtgcg ccgccgcccg gtgcggcaga gggggctctg acctggtcgg
    22381 cggccgcctt catctgcgaa gggcgcagca ccatggtctt ctcgaaatcg gccttctcgc
    22441 cgccacccgt agtcgcgggc gccgactcgc cgatgaactt gagcttgtac ttgccgagct
    22501 cgacgacgtc gttgttctgc aggaagtgct tcttgatcgg gttgccgttc acgacggtgc
    22561 cgttggtgct gccgaggtct tcgaggaacg agtcgttgag gatggtgacg atcaccgcgt
    22621 gctcgccgct caccgcgagg ttgtcgatct ggatgtcgtt gtgcggcttg cggccgatgg
    22681 tggtgcgctc cttcgtcaag gggatctcct tgagcaccag gccatccatg ctgagtatca
    22741 gtttcgccat agcgggtccc tgagggagct tattttagcc agccgaaaac cttgcccatg
    22801 acgccgcgcc ggccgggata ctcccgtagg acgcggacca gtataaccga aacgttatcc
    22861 cgccccccgt tgtcgttggc catctgcacc agctgctgcg cggcgagctt gaggttgccg
    22921 ccgagggtct gcagcgtcat gccgatgtct tcgtcctcga ccatgtcgca caagccatcc
    22981 gagcagagca ggtagatgtc tcccgctctg gtggtgtact cgtggatctc gggctcgacc
    23041 gtcgggtcga tgccgagcgc cttcgtcacc aggttcttgt gctgcgcctt cttcgcctgc
    23101 tctggggtga tgattccgga atcgatctgc tcctgcagca gcgagtggtc gcgcgtgacc
    23161 tgggtgaact tgccgtcgcg cagcatgtac agccgcgagt cgccgaggtg cgccaccagc
    23221 atccggttgt cgtagtaaag gcagaccacg agcgtcgtgc ccatgccggc gtattgcggc
    23281 tggctctgcg cggcctggta gatcgagctg ttcgccttga gcacctgctc gcgcaccagg
    23341 cgcgcggcga tttcctcgct ggtgcggctg tcgacgtcgt agggcttggt gctggcgagg
    23401 atctggcgca tctcgctgac gatcaccgtg gtcgccatgc cgctcgccac ctcgccggcg
    23461 ttgtagccgc ccatgccgtc ggcgagcacc accaggccgt tcccggtatc ggcggcgatc
    23521 gagtcttcgt tgtgcgagcg caccatcccc ggatcggtgc acgtcgcgat ctcgaggttc
    23581 tggctgagat ccatgctcgc tacgacccgc ttttcttcgc caaacgcaac ttcaaaagcg
    23641 ccctccccgc ttatccgcga ggcgcattgt tgcagcccaa agcgagatga gtcaacgatt
    23701 tagctgagga tcgcgcctgc cttgatcgcc ccggcggaaa cgagttccgc cgcagccact
    23761 ttcttgccgt cgccgagctt cgcgcgcagc acctcgatgc gcccgccctg cgcggtcacc
    23821 tggaaggact tgtcgccgac ctcgaccacc tcgccaatct tgcccctgac cgcgccgaag
    23881 gtgcgcaccg ggtgtttgcg cgcatcgaac acctgcaatt cctttccctc gagggtggac
    23941 caggcgcccg gcgccggatt gcacccgcgc accaggttgt agacgaagtc gacgtggttc
    24001 gcccagttga tgcgcgcctc ggccttgcgg caccagccct cgtaggtagc ctgggactcg
    24061 tcctgcacca cctccttgtg cttgcccgcg aggaccagat ccgcggcctc gatcatcgcc
    24121 ttcaccccca tcggaaagag gcggtcgaag tacaccgtgc cgagcgtgtc gtccgggctg
    24181 atcggggttt ccttctgcag cacgaccgga ccttcgtcga ggccgtcggt cggccggaaa
    24241 atcgtcaggc cggtcttgct gtcgccgcgg atgatcggcc agttgatcga cgacggcccg
    24301 cggtacttgg ggagcagcga agggtggtac tggatcgtgc cgcgcttggg gatattgacg
    24361 aaatcctgcg gcgcgaacta cagcacgaac gccatgatgc cgagatcgac gttgaggttt
    24421 ttcagcgcct gctttgcctc ggcactcttc agcgacgcga actgaaaaac cttgaccccc
    24481 ttctcctgcg ccgccacctt gagcgcgtcg ggcctcgccc ccggcttctc cggcgcgcag
    24541 aacacgccgg ccacctcgtc cttgcgcgcg aggaacgctt ccagcacgct cttgccgaag
    24601 tcctgctgcc cgacgatcgc gattttcatc aagccgcttt cttcggcggc accgagaacg
    24661 cgccggcgct cttcagcttg gcgatcttgt ctttttcgta gccgaggacc tgctgcagga
    24721 tctcgtcggt gtgctcgccg agcgtcggcg agcgccggat ctgcgccggc gagtccgaca
    24781 gcttgatcgg cattcccacg ttccaccact tgccgcgctg cggatggtcg agctcgaccc
    24841 acatgtcgcg cccgcgcacg tgctcgtcgg tggcgaggtc ctcggtcgac atgatcggcc
    24901 cgcacggcac gtccagggga ttcaggatcg ccatcagctc gcgcttggtg tacttctcgg
    24961 cgaacttgtt gatcaaggtc cacatcaggt tctgattttt tcttctatct gaaattttcg
    25021 cgaaccgggg gtcgcacgcc atctcgggca tgccgacgtc cgggccgatg cgcttggcga
    25081 ggtcgtccca caccgcctcc tgcaccacga cataaagcca gtcgttgggg ccgtgcggct
    25141 tgcagtggat ggcgttgccg agctggccgc cgcccgagtc gttgccggcg cgcggcacgt
    25201 cgcccatgcc cttgtaggtc ggcaccgaaa actccgacag atcgccgcgc gtgaggcgct
    25261 ggtggtcgcg gaatttcacg cggcagagat tcatcacgcc gtccatcatc gcgacctcga
    25321 cgtattggcc ttgtccggtt ttctccgcct gcctcaaggc agccaagaga cctattgcca
    25381 aatgcaaccc ggtgccgctg tcgccgatct gcgcgccggt gacgaagggt gggccgtccg
    25441 gcacgccggt ggtgctcatc gccccgccca tcgcctgcgc cacgttctcg taggccttga
    25501 agtcggaata cggcccgctc gagccgaagc ccttgatcga gcccatcacg atcgccgggt
    25561 tcagctggtg aattttctcc cacgggaagc cgaagcggtc gagcacgccc gggccgaagt
    25621 tctccatgat gatgtgcgac ttcttcagca ggtcgacaaa cacctgcttg ccctcttccg
    25681 tcttcatatt gacggtaatc gagcgcttgt tgcagttgag catggtgaag tagagcgagt
    25741 cggcgttcgg cacgtcgcgc agctggccgc gcgttgcgtc gccctgcggg ttctcgaact
    25801 tgatgacgtc ggcgcccaac cacgccagca gctggctgca ggtcggacct gcctggacat
    25861 gcgtcatgtc cagaatgcga acccctttca gcgcttccat tactttttct tccctgcagt
    25921 tggattgaga ctcgtgatgc ggccgctttc ggtgccggcc gtttcgtcga tcacggcatt
    25981 gatgagcgtg ggcttcctcg agcggatcgc gtcttccatc gccttgcgca gctccgccgg
    26041 cgtggtggcg tttacgccca cgccgccaaa ggcttccatc agcttgtcgt agcgcgcgcc
    26101 cttcacgaac acggtgggcg cgggatcgcg accgttggga tcgacgtcgg tgcccttgta
    26161 gactccgtta ttgttcatga tcacgatgca caccggcatg ttgtagcggc agatcgtctc
    26221 cacttccatg ccggaaaagc cgaaggcgct gtcgccctcg atggcgatga cctgcccctt
    26281 ggtcacggcg gccgcggcga cgcagtagcc catgccgacg cccatgatcc cccaggtgcc
    26341 gacgtcgatg cgcttacgcg gcttgtacat gtcgacgatc gagcgtgtga agtcgagcgc
    26401 gttcgcgccc tcgttcacga acatcgcgtc cgggttcgcc ttcacgatgt cgcgcactac
    26461 gttgagcgcg ctgtggaaat tcatcggcga gggattcttc gccagcgtct cggccatctt
    26521 ggcgacgttg gtggtctttt tctcggtgat tgcattcagc cattcggccg ggggtcttgc
    26581 ccaattgctg cccattccgg ccagcaaggc ggaaacgcac gatccgatat ccccgaccag
    26641 cggcgcgtcg atgcgcacgt tgctgtcggc ttcctgcggc gagatgtcga cctggatgaa
    26701 cttctgaccg ccccagtcct tgtggccctt gccgccccag gtcttgccct tgccgtgcga
    26761 cagcagccag ttgaggcgcg cgccgatcag catcaccacg tcggcggccg gcagcacgaa
    26821 ggagcgcgcc gcggaagcgc actgctcgtg cgtgtcgggc agcaggccct tcgccatcga
    26881 catggggaga taggggatgc cggttttttc gaccaacgca cgaatttcag catcaacctg
    26941 ggcgtacgcc gcccctttgc ccagaacgat cagcggtttt ttcgcaccct tgagcagatc
    27001 gagcgcgcgc ctgactgcgt ccggggcggg aatctgcttc ggcgccgcgt cgacgacctt
    27061 gatgagcgag ttttttccaa gagaagaatc tatggattgc gcgaacagct tggcgggcag
    27121 gtcgaggtag acgccgcccg ggcggccgga caccgcggcg cggatggcgc gagcgacgcc
    27181 gacgccgatg tcctcggcgt gcagcacgcg gaacgcggcc ttggcgagcg gcttggcgat
    27241 ggcgagctgg tccatctcct cgtagtcgcc ctgctgcagg tcgacgatct cgcgctcgct
    27301 cgagccgctg atcaggatca tcgggaagca gttgaccgtg gcgtgcgcga gcgcgttgag
    27361 gccgttcagg aagcc
//
LOCUS       DATBSZ010000182        20828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_99158, whole genome shotgun sequence.
ACCESSION   DATBSZ010000182 DATBSZ010000000
VERSION     DATBSZ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20828
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99158"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1240
                     /locus_tag="VIG70_11740"
     CDS             122..1240
                     /locus_tag="VIG70_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY8251272.1"
                     /translation="MSASRRKFLRGAAAATGAATLGFPMIAKAQGPISMRWQSTWPAK
                     DIFHEYANDFAKKVNDMTGGDLRIEVLPAGAVVPAFGLLDAVTKGTLDGGHGVLVYHY
                     GKQNALALWGSGPGFGMDANQLLAWHKYGGGKDLLNKLYASIGANVVSFPYGPMPTQP
                     LGWFKKPITKVDDFKGLKFRTVGVSIDVFTGLGAAVNALPGGEIVPAMDRGLLDAAEF
                     NNATSDRLLGFADVSKVCMLQSYHQNAEQFEIMFNKPKFDALPAKIKAIIENAVEAAS
                     SDMSWKAVDRYSKDYIELQTKDKVRFYKTPDSILARQLETYDAAAKKRSDNAMWVEIE
                     KSQRVFAERAVRWYLDTQVSPRMAYTHYFGKKPAAKKA"
     gene            1295..1912
                     /locus_tag="VIG70_11745"
     CDS             1295..1912
                     /locus_tag="VIG70_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY8251273.1"
                     /translation="MQNTLLLVDRLSTWIGQAFSWFIVALTLQVSWEVFSRYALDAPH
                     SWAFDVMIMFYGTLFMMAGAYTLAKNGHVRGDVLYGFFRPRTQATIDLILYIVFFIPG
                     VFALTYAGYYYAADSWHIRETSNVTAEGPPVYPFKMIIPFAGAILLLQGIVEIIRCVI
                     CLKQGYWPSREQDVEEVDVAKLKEELHVKDEDIAKLDVYVTKDKA"
     gene            1948..3924
                     /locus_tag="VIG70_11750"
     CDS             1948..3924
                     /locus_tag="VIG70_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY8251274.1"
                     /translation="MALIVTTVLVLTPWGSLMDAHLSKEDLGTLGLLMLALIVVAIML
                     GFPTAFTLMGMGVFFAWLAYRSVNPQMAVNQVLDLFVQRTYGVMSNDVLIAIPLFLFM
                     GYLVERAQLIDRLFKSLHLATSRVPGSLAVATIITCAIFATATGIVGAVVTLMGLLAF
                     PAMLKAGYNIKVAAGSITAGGCLGILIPPSVLLIVYGATAGVSVVQLYAGAFFPGLML
                     ASLYLGYIILLAKWKPSLMPPLAESERRVPLPPSVQALATLGSNALAAIGRVLAGRGV
                     PGVPRRTILTQLVVTLIPALAIVAILGTSYRIATAPDVQASTAGLIEAGGAIAADASE
                     SEQSSGLIGAPGAEEEPQEEKPQELGAPAAAEEAKPAAEEKKPAAAAKRVPPPAWFYV
                     LLAIGLGAILLIYWLWNWERLEVFKLLLTSFFPLALLILAVLGSIVAGLATPAEAAAV
                     GAFGAFILTAVYRFIAHWRERSQSGASSVEVVWRTMRQFGRIIKESSFLTAKTSAMVC
                     WLFVGSGIFAAAFALLGGQELIEKWVLSLNMTPLQFMILAQCIIFILGWPLEWTEIII
                     IFMPIFIPLLPHFGIDPLFFGLLVALNLQTAFLSPPVAMAAFYLKGVSPPHVTLNQIF
                     AGMLPFMGIQVIALILLYTFPQIGLWLPSVMYAR"
     gene            3914..5251
                     /locus_tag="VIG70_11755"
     CDS             3914..5251
                     /locus_tag="VIG70_11755"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8251275.1"
                     /translation="MRDERSLALSAADAAQQIRDGRLTSEEVVQASLERIRALEPKVQ
                     AWTFLDEEHALAQARALDERKRNGEPIGPLHGVPVGIKDIIDTADMPTENGAALHKGR
                     TPREDAAVVRMLRAAGAVILGKTVTTECAYFNPGKTRNPHNPEHTPGGSSSGSAAAVG
                     AGMVPLALGSQTAGSVIRPAAFCGAHGFKPTHGLIPRTGILQLSRTLDHVGLFSRSID
                     DLALLLEQLAGYDEGDSDTRPRARLPFLELSREDPPVEPMLAFVKTPHWDRTDADTKE
                     AFQELVETLGKQVEEVELFPSAMEAWDWHRTIMEAEMAANLEPLWVAGKEKLSEKLRG
                     LIERGREVRAVDHQRALRRIGPSVESLDELFQQRYDAILTPPALGTAPKGLGATGDPV
                     FCSIWTLFGMPAITLPLMQGENGLPLGVQLVGRKGYDARLLRTARWLEKKVAS"
     gene            complement(5248..5754)
                     /locus_tag="VIG70_11760"
     CDS             complement(5248..5754)
                     /locus_tag="VIG70_11760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8251276.1"
                     /translation="MDIVRAAKSWHNRHMALAQQRIVKPETAPALYRLLSDSALSRAA
                     LGACGLPLAMLDARIGSRPVTYVNTAFEGFFGYRAGEALGRPLAALLFHGDEPLVHRL
                     LAESSSRWKLRAWGKDGAVRQVEISFGAVHGADGQLTHWVVAFADRSEVEKLRAELEG
                     LKALAAAP"
     gene            complement(5803..6693)
                     /locus_tag="VIG70_11765"
     CDS             complement(5803..6693)
                     /locus_tag="VIG70_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251277.1"
                     /translation="MRVRDAILAIYAARYPGVEFAPLERAFADFKALFEGGYPGYLAC
                     DTLYHDMRHTLDMTLALARLVDGYERACTTADRVGPRRAILGVLIALLHDSGYLRRAS
                     ETAVENGAVFTKTHVSRGADFISAYLPKLGFGAEAATAARLVHFTGYEMEIDDIRVED
                     PKDRVIGCLVGTADLLGQMSDRMYLEKCREFLYKEFVWGKIARERLPDGHELVRYASP
                     EDLIIKTPGFYEYVARTRIQKKLGSVDRYAEAHFDGPSPYQSEIDRNMDFLRETIETA
                     DFSRLRRSCYSLSVTTRRAA"
     gene            complement(6799..7142)
                     /gene="rnpB"
                     /locus_tag="VIG70_11770"
     ncRNA           complement(6799..7142)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VIG70_11770"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(7223..7942)
                     /locus_tag="VIG70_11775"
                     /pseudo
     CDS             complement(7223..7942)
                     /locus_tag="VIG70_11775"
                     /inference="COORDINATES: protein motif:HMM:NF022673.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
     gene            complement(7855..8889)
                     /gene="pyrC"
                     /locus_tag="VIG70_11780"
     CDS             complement(7855..8889)
                     /gene="pyrC"
                     /locus_tag="VIG70_11780"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY8251278.1"
                     /translation="MMDRLKLRRPDDWHLHLRDGAALASVIADSARRFARAIVMPNLK
                     PAVRTTAQALDYRERILGALPDGASFDPLMTLYLTDDTPPEEIARAKLSGHVHGVKLY
                     PAGATTHSDEGVTRLSRCFHALEKMEELGLPLLVHGESTDPAIDVFDREQAFIEETLG
                     PMLERFPRLKVVLEHITTREAAQYVEVTGPNVAATITAHHLLMNRNALFLGGIRPHHY
                     CLPVLKRETHREALVEAATSGNPKFFLGTDSAPHARDAKEGACGCAGIYTAHAAIELY
                     AAAFEEAGALDKLEGFASAFGAGFYGLPLNRDTITLERAEWRVPERLAFGGEELVPLR
                     AGETIPWKLV"
     gene            complement(8891..9697)
                     /gene="rsmI"
                     /locus_tag="VIG70_11785"
     CDS             complement(8891..9697)
                     /gene="rsmI"
                     /locus_tag="VIG70_11785"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY8251279.1"
                     /translation="MVATPIGNLSDASPRSLDTLKRADIVACEDTRTTRTLLSRHGVA
                     ARTVALHQHNERRAAAALIAALREGKSIALVSDAGTPALSDPGAWLVAEAHRAGIRVS
                     PVPGPSAAAAALSVSGFAAPQFLFAGFLPAGAAARRKALDALDLPWPVVIYEAPHRIA
                     DTLEDLRQKFGDAREILVARELTKKFEEVARMPLSGAPAWLAGRAERSKGEFVLVLAP
                     GTAREAQPGIDPENVLEILLEAVAPSEAARLAAKITGVPKNVLYRKTLKS"
     gene            9765..10103
                     /locus_tag="VIG70_11790"
     CDS             9765..10103
                     /locus_tag="VIG70_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY8251280.1"
                     /translation="MNAEGARAEDLCAGLLRAAGLVLIERNWRCRHGEIDLIAEERGM
                     LVFAEVRMRSRPGFGGAGESVTAAKRQRLLAAARLYLARRPEALCRFDVFLVDGATSN
                     VQWIRDAFGE"
     gene            10076..10453
                     /locus_tag="VIG70_11795"
     CDS             10076..10453
                     /locus_tag="VIG70_11795"
                     /inference="COORDINATES: protein motif:HMM:NF020376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HEY8251281.1"
                     /translation="MDPRRLWRIGILLTLAWGDPHAEERVRLLVQNSPLAGFRYHAAA
                     EMWRELRVGDPLELAREPGNPHDANAVRVLWRGHMLGYVPRRENGALAWGLDRGAPLR
                     ARISALADHPNPARRVRFEVYVE"
     gene            10464..11060
                     /locus_tag="VIG70_11800"
     CDS             10464..11060
                     /locus_tag="VIG70_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEY8251282.1"
                     /translation="MAQAEISQRIAAHFAESANLKLAAAQALTEPLERASRLMVDTLR
                     GGGKILSCGNGGSAADAQHFAAELVNRFERERPPLAAIALSTDTSSLTSIANDYAYQQ
                     VFSKPLRAFGRRGDALLAISTSGNSANVLDAIHAAHEIGVRVVALTGNGGGKMGAALR
                     PDDAHLCVPHRTTARIQEVHLLCIHCLCDAIDTLLFPV"
     gene            11024..11653
                     /locus_tag="VIG70_11805"
     CDS             11024..11653
                     /locus_tag="VIG70_11805"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8251283.1"
                     /translation="MRRNRYFVIPGLIAVLCLQGCPAIIAGGSVVAVTSLEDRRTTGT
                     QLDDSGIESNAASRIGERIGERAHVNVTSFNRAVLLTGEAWDEATRAELEKIVATVPN
                     VRNVTNEVQVAGASSATSRANDTAITARVKGRFVNVKDLNPLHVKVVTEAGVVFLLGL
                     VTETEAAAATDLARTTGGVRKVVKVFEYCKSTEAPCKPAPPAAPPKSQR"
     gene            11677..12132
                     /locus_tag="VIG70_11810"
     CDS             11677..12132
                     /locus_tag="VIG70_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY8251284.1"
                     /translation="MHDPAALQNWIGRLARPLVFTNGVFDLLHRGHVTYLARARALGA
                     ALLVALNSDKSVRNLGKGNDRPLNPLADRLAVLAALECVDAVTWFDDDTPERLIAASR
                     PQVLAKGGDWPAERIVGAGGVLARGGQVVSIPFEHQRSTTDLVGRIRKG"
     gene            complement(12119..12562)
                     /locus_tag="VIG70_11815"
     CDS             complement(12119..12562)
                     /locus_tag="VIG70_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8251285.1"
                     /translation="MARLVEATGEAQVAVVRELFAEYAHGVDEPCCFTGFERELDSLP
                     GEYRLFLLDDAAGCVAVRFLDRQTAEMKRLYVRAAQRGRGLGRVLAEAAITAAREAGC
                     TRIVLDTLPKMREAQALYGMLGFKPIAPYLENPTPGALCFELSLS"
     gene            12576..13100
                     /locus_tag="VIG70_11820"
     CDS             12576..13100
                     /locus_tag="VIG70_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251286.1"
                     /translation="MRLHQLKVDYDAEQDRLLMLVATSEGVELRLSLTRRFVKLLWPL
                     LVKLAEEASPRIRTQANPEARKALLGLEHEHAVSKADFSKPYDAAGSERPLGEAPLLL
                     ARIQTGHDRNGQPVVALHPAEGQGITLTFDSVLLHSVCRLLQSAVKKSDWDMELKLPG
                     TEPQEGGERVRTLN"
     gene            13201..14553
                     /locus_tag="VIG70_11825"
     CDS             13201..14553
                     /locus_tag="VIG70_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8251287.1"
                     /translation="MQEQAIPLVLGGRDLMAGAQTGTGKTAAFALPILQRFAPLASAS
                     TSPARHPVRALVLTPTRELAVQVAESFRDYGKNLPLRSTVVYGGVDMNAQIEQLRKGV
                     EILVATPGRLLDHVQNKTVMFNQVSVLMLDEADRMLDMGFLPDIKRIIALLPRERQNL
                     LFSATFPDEIRTLAKTLLRNPAEVQVAARNAAAENVSHVLHPVAREKKRELLAYLIQT
                     RGLRQVLVFTGTRIGANRLAHQLRRDHIHADAIHGDKSQIEREAALADFKSGKTAVLV
                     ATDVASRGLDIEGLPQVINFDVPHSPEDYVHRIGRTGRAGLPGEAISLVAPQDHDAMA
                     AIERLIKKRIERQLIPGFGPSGATVAAMMSDQPSRKEKDRRPAPSTQDRRPAPSTQGR
                     RQTPSTPPKRQIDPIFSKPYEPPAAASPAPAANQEPPQHPRRKRPQVAALLGGMKKAS
                     "
     gene            complement(14554..15291)
                     /locus_tag="VIG70_11830"
     CDS             complement(14554..15291)
                     /locus_tag="VIG70_11830"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY8251288.1"
                     /translation="MRLKEKTAIVTGAGSGFGAGIAKRFAEEGARVIVADINAQAGER
                     VAREVEGKFIQADVTRGDDWRKLVGAAGERLDIIVNNAGWTHRNKPYLEVTEAEFDRV
                     YAVNVKSIYLSALHAVPVFRKRGRGCFVNIASTAGLRPRPGLTVYNSSKGAVILMSKS
                     MAGEFGPDRIRVNCVNPVFSPDTALSAEFAGGAVTEEAKKRFLATVPLGRFSTPLDVA
                     NACLYLASDEAEFISGVCIEVDGARCV"
     gene            complement(15288..16394)
                     /locus_tag="VIG70_11835"
     CDS             complement(15288..16394)
                     /locus_tag="VIG70_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8251289.1"
                     /translation="MESVRVVDSLGSVDAAEWNALAGSQPFVRHEFLSALMETGCASA
                     RSGWAPQFLLVRRAGALVAAMPLFAKSHSYGEYVFDWAWAEAHERHGVEYYPKLLSAI
                     PFTPVRGPRLMGNSPEVLLEAALELARNTSSLHILFPLEEEIPHLVGRGMLLRRTVQF
                     HWRNAGYADFDAFLNSMTRERRKKIRQERNRIREAGIAFSWWEGSAIERRHWEFFNRC
                     YRRTYAEHRSSPYLSLDFFLRLGAALPGNLALLVAERDGRPIASSFFFKDESTLYGRY
                     WGALEHVPLLHFECCYYQAIEYAIRCGLQVFEGGAQGEHKLWRGLLPVEAPSAHWLAH
                     PKFARAVESFLEREGTGVTRYVDELCEHSPFKKP"
     gene            16498..16836
                     /locus_tag="VIG70_11840"
     CDS             16498..16836
                     /locus_tag="VIG70_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY8251290.1"
                     /translation="MKKIEAIVKPFKLDEVREALSEVGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVVANNQVESAIDAIVRSARTGKIGDGKIFVTSVEHVVRI
                     RTGETNEAAV"
     gene            complement(16833..17303)
                     /locus_tag="VIG70_11845"
     CDS             complement(16833..17303)
                     /locus_tag="VIG70_11845"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY8251291.1"
                     /translation="MDSDFRKALAGVVPLVKARRAVRERPRPAPLPRETIAPEDTTES
                     GAELAFVRPGYSPALLRKLRRGRWVVEDSLDLHGMNRSEAAGAVREFLHGCRERRLGC
                     VRIVHGKGLGSRNREPVLKGKVYHWLKLRDEVLAFSQAPTADGGAGAVLVLLRS"
     gene            complement(17296..18246)
                     /gene="trxB"
                     /locus_tag="VIG70_11850"
     CDS             complement(17296..18246)
                     /gene="trxB"
                     /locus_tag="VIG70_11850"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY8251292.1"
                     /translation="MDTRNARLLILGSGPAGYTAAVYAARANLRPVLIAGVAQGGQLM
                     TTTDVDNWPADAAGVQGPDLMQRFQKHAERFETEVVSDQIHTARLRERPFVLEGDTAR
                     YTCDALVIATGATAKYLGLPSEQKFMGRGVSACATCDGFFFKGQDVVVVGGGNTAVEE
                     ALYLANLCSSVVLIHRRDKFRAEKIMVDKLMKRVAEGKIRLELDAVVEEVLGDSGGVS
                     GVRVRNLKGGAVKTIAAKGFFVAIGHTPNTGIFAGQLEMKDGYIITRGGREGLATATS
                     IPGVFAAGDVQDHVYRQAVTSAGSGCMAALDAEKYLDGHG"
     gene            18271..18939
                     /locus_tag="VIG70_11855"
     CDS             18271..18939
                     /locus_tag="VIG70_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8251293.1"
                     /translation="MVSTVSTAVLKTVPLFTTFPEDQLRMLTTMVTRKSAPRSTTIMA
                     GGDATDSLYIVLSGRLKVMMSDSEGKEVILTILGPGEFFGEMGLIDDEPRSATVMTIE
                     PCELLSIAKRDFKKCLSENSEMAMAVMRSLVKRLREADRKIGSLALLDVYGRVARLLL
                     DMAENVDGEKVVTKRLPKQDIAKMIGASREMVSRVMKDLQTGGYIEMRGSTIVLRDTI
                     LLPE"
     gene            18962..>20828
                     /locus_tag="VIG70_11860"
     CDS             18962..>20828
                     /locus_tag="VIG70_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY8251294.1"
                     /translation="MAVASKGAPLPAKLAGLLREAKWLLLIALALYLLLILATFHRAD
                     PGWSHSATDAVARNAGGAFGAWLADLLLYLFGVSAYWWAALCAYLVVWGYRRMDGVRL
                     AGAVAGFSLLLLSSAALERLRLHSLGAELPLAPGGLIGEALAGAFSGALGFTGATLAL
                     LTAAAIGFSLFIGVSWLTLFEGVGRLLEKAYAAALGAWERRRDRKLGSRAREQREAVV
                     ESGKRREEEHPPLRIEPALAEIKKSERAQKEKQAPLFEHLPDTPLPPLKLLDEAATGG
                     EAIAAETLEFTSRLIEKKLSDFGVTVKVLAAYPGPVITRYEIEPAVGVKGSQIVNLVK
                     DLARALSVVSIRVVETIPGKSCMGLEIPNPHRQIVRLTEILGSEAYHGMHSPLALALG
                     KDIAGRPVVADLARMPHLLVAGTTGSGKSVALNAMILSLLYKSELRDVRLILIDPKML
                     ELSVYQDIPHLLAPVVIDMKQAVNALTWCVAEMDRRYKLMNWIGARNLSGYNHRVAEQ
                     EFEDPFALDAGKAEKLTPLPQVVVVIDELADLMMVVGKRVEELIARLAQKARAAGIHL
                     ILATQRPSVDVITGLIKANIPTRIAFQVSSKVDSRTILDQSGAEALLGSGDMLYLP"
BASE COUNT         3369 a         7065 c         6995 g         3399 t
ORIGIN      
        1 gggcagaagc tggcatcttg caccgcggac tacaccgtgg ccgccgactg ttctagaatc
       61 gccggctagg cccgaatctg atcgtcatta ccccgttcac cctaggagga ggagaaaatc
      121 aatgagcgct tcacgccgta aattcctgcg cggtgccgca gccgccacgg gcgccgcgac
      181 cctcggcttc ccgatgatcg ccaaggccca aggtccgatc tcgatgcgct ggcaaagcac
      241 ctggccggcg aaggacatct tccacgagta cgcgaacgac ttcgcgaaga aggtgaacga
      301 catgaccggc ggcgacctca ggatcgaggt gctgcccgcc ggcgcggtag tgccggcgtt
      361 cggcttgctc gacgcggtca ccaagggcac gctcgacggc ggccacggcg ttctcgtgta
      421 ccactacggc aagcagaacg cgctcgcgct gtggggctcg ggcccgggct ttggcatgga
      481 cgcgaaccag ctcctcgcct ggcacaagta cggcggcggc aaggacctgc tgaacaagct
      541 ctatgcctcc atcggcgcca acgtggtgtc gttcccctac ggcccgatgc cgacgcagcc
      601 gctcggctgg ttcaagaagc cgatcaccaa ggtcgatgac ttcaagggcc tcaagttccg
      661 caccgtgggc gtgtcgatcg acgtgttcac gggcctcggc gccgcggtca acgcgctgcc
      721 cggcggtgag atcgtcccgg cgatggaccg cggcctgctc gacgcggcgg agttcaacaa
      781 cgcgacttcg gaccggctcc tcggcttcgc cgatgtctcc aaggtatgca tgctgcagag
      841 ctaccaccag aacgccgagc agttcgagat catgttcaac aagccgaagt tcgacgccct
      901 gccggcgaag atcaaggcga tcatcgagaa cgcggtcgag gcggcctcgt ccgacatgtc
      961 gtggaaggcg gtcgaccgct attccaagga ttacatcgag ctgcagacca aggacaaggt
     1021 gcgcttctac aagacgcccg actcgatcct cgcgcgccag ctcgagacct acgacgccgc
     1081 ggccaagaag cgctcggaca acgcgatgtg ggtggagatc gagaagtcgc agcgcgtctt
     1141 tgccgagcgc gcggtgcgct ggtatctgga tacgcaggtg agtccgcgca tggcgtacac
     1201 gcattatttc ggcaagaagc cggcagcaaa gaaggcctaa gagcctgagc gacccatcag
     1261 ccatccggcg cgagccggat ggctgtttca tttaatgcaa aacacgctac ttctcgtcga
     1321 ccggctcagc acctggatcg gccaggcgtt ttcctggttc atcgtcgcgc tgacgctgca
     1381 ggtgtcctgg gaggtgtttt cgcgctacgc gctcgacgcg ccgcactcct gggcgttcga
     1441 cgtgatgatc atgttctacg gcacgctctt catgatggcc ggcgcctaca cgctggcgaa
     1501 gaacggccac gtgcgcggcg acgtgctcta cgggttcttc cggccgcgca cgcaggcgac
     1561 catcgacctc atcctgtaca tcgtgttctt catccccggc gtgttcgcgc tgacttacgc
     1621 aggctactac tacgctgccg acagctggca catcagggaa acctcgaacg tcaccgccga
     1681 ggggccgccg gtctacccgt tcaagatgat tatcccgttc gcgggggcca ttctcctcct
     1741 gcagggcatc gtcgagatca tccgctgcgt gatctgcctc aagcagggct attggccgtc
     1801 gcgcgagcag gacgtcgagg aagtcgacgt cgcgaagctg aaagaggaac tgcacgtcaa
     1861 ggacgaggat attgcgaagc tcgacgtcta cgtcacgaag gataaggcgt gatcgtccgc
     1921 agggagctgt ggttcggctt cgggctcatg gccttgatcg tgaccacagt cctggtgctt
     1981 acgccttggg gcagcctgat ggatgcccat ttgagcaagg aggacctcgg caccctcggg
     2041 ttgctcatgc tggcgctgat cgtggtcgcc atcatgctcg ggtttcccac ggccttcacg
     2101 ctcatgggca tgggcgtgtt cttcgcctgg ctcgcctatc gcagcgtcaa cccgcagatg
     2161 gcggtgaacc aggtgctcga cctgttcgtg cagcgcacct acggtgtgat gtcgaacgac
     2221 gtgctgatcg cgattccgct gttcctgttc atgggctacc tggtcgagcg cgcgcagctg
     2281 atcgatcggc tgttcaagag cctgcacctc gccacctcgc gcgtgccggg gtcgctcgcg
     2341 gtggcgacca tcatcacctg cgccatcttc gccactgcca cggggatcgt cggcgcggtg
     2401 gtgacgctga tgggcctgct cgcgtttccg gcgatgctca aggccggcta caacatcaag
     2461 gtcgccgcag ggtcgatcac cgccggcggc tgcctcggca tcctgatccc gccctcggtt
     2521 ctgctgatcg tctatggcgc gactgctggt gtgtcggtgg tgcagctcta tgccggtgcg
     2581 ttctttcccg gactcatgct ggcctccctc tacctcggct acatcatcct gctcgcgaaa
     2641 tggaagccga gcctgatgcc accgcttgcc gagagcgagc ggcgcgttcc gctgccgccc
     2701 tcagtccagg cgctggcgac ccttggcagc aatgcattgg ccgcgatcgg acgcgtgctt
     2761 gccggccgcg gcgtgccggg tgttccgaga cgcacgatcc tcacgcagct cgtcgtcacg
     2821 ctgataccgg cgctggcgat cgtcgcaatc ctcggcacga gctaccgcat cgcaaccgct
     2881 cccgacgtgc aggcgagcac ggcaggcctg atcgaggccg gcggggcgat cgcggccgac
     2941 gccagcgaat cggagcagtc gagtggcctg atcggcgcgc ccggcgccga ggaggaaccg
     3001 caggaagaga aaccgcagga gctgggcgcg cctgccgcag ccgaagaggc gaagcccgct
     3061 gccgaggaga aaaagcctgc cgccgcggcg aaacgcgtgc cgccgccggc ctggttctac
     3121 gtcctgcttg ccatcggtct gggggcaatc ctgctcatct actggctatg gaactgggag
     3181 cggctggaag tgttcaagct gctgctcacc tcgttctttc ctctggcgct gctgattctc
     3241 gcggtgctgg gctcgatcgt cgccggcctc gcgacaccgg ccgaggcggc ggcggtgggt
     3301 gcgttcggcg ctttcatcct gacggcggtc taccgcttca tcgcccactg gcgcgagcgc
     3361 tcgcagagcg gagcctcgag cgtcgaggtc gtctggcgca ccatgagaca gttcggcagg
     3421 atcatcaagg agtcctcgtt cctcaccgcc aagaccagcg ccatggtgtg ctggctgttc
     3481 gtcggctcgg gcatcttcgc ggccgcgttt gccctgctcg gcgggcagga gctgatcgag
     3541 aagtgggtgc tgtcgctcaa catgacgccg ctgcagttca tgatcctggc gcaatgcatc
     3601 atcttcatcc tcggctggcc gctcgagtgg accgagatca tcatcatctt catgccgatc
     3661 ttcatcccgc tgctgccgca cttcggcatc gacccgctgt tcttcgggct gctggtggcg
     3721 ctcaacctgc agaccgcgtt cctgtcgccg ccggtggcga tggcggcgtt ctatctcaag
     3781 ggcgtgtcgc cgccgcacgt gacgctcaac cagatcttcg ccggcatgct ccccttcatg
     3841 gggatccagg tcatcgcgct gatcctgctc tatacgttcc cgcagatcgg gctgtggctg
     3901 ccgagcgtca tgtatgcgcg atgagcgctc gctggcgctc tccgcggcgg acgccgcgca
     3961 gcagatccgc gatggcaggc tgacctccga agaagtggtg caagcctccc tcgagcgcat
     4021 ccgggcgctc gagccgaaag tgcaggcctg gaccttcctc gacgaggagc acgcgctggc
     4081 gcaggcgcgt gcgctcgacg agcgcaagcg caacggcgag cccatcgggc cgctgcacgg
     4141 cgttccggtc gggataaagg acatcatcga taccgccgac atgccgaccg agaacggcgc
     4201 ggcgctgcac aaagggcgca cgccgcgcga ggacgcggcc gtggtgcgca tgctgcgcgc
     4261 ggcgggcgcg gtgattctcg gcaagacggt cactaccgag tgcgcctact tcaacccggg
     4321 caagacgcgc aatccgcaca accccgagca cacgccgggc ggctcctcca gcggctcggc
     4381 ggccgcggtc ggcgccggca tggtgccgct cgcgctcggc agccagaccg ccggctcggt
     4441 gattcggccg gcggcgttct gcggcgcgca cggcttcaag ccgacgcacg ggctgattcc
     4501 gcgcaccggc atcctgcagc tctcgcgcac cctcgaccac gtgggactct tttcccgcag
     4561 catcgacgac cttgccttgc ttctcgagca actcgcgggc tacgacgagg gggactccga
     4621 cacgcggccg cgcgcccggc ttccttttct cgagctttcc cgcgaggacc cgccggtcga
     4681 gccgatgctg gccttcgtga agacgccgca ctgggatcgc accgacgccg acaccaaaga
     4741 ggcgtttcaa gagctggttg aaaccctagg caagcaggtg gaggaagtgg agctctttcc
     4801 gtcggccatg gaggcctggg actggcaccg gaccatcatg gaagccgaga tggcggccaa
     4861 cctggagccg ctctgggttg ccgggaagga gaagctgtcc gagaagctgc gtggcctgat
     4921 cgagcgcgga cgcgaagtgc gagcggtcga ccatcagcgc gcgctgcgcc ggatcggccc
     4981 gagcgtcgag agcctggatg agctgttcca gcagcgctac gacgcgatcc tgacgccgcc
     5041 cgcgctcggc acggcgccca aaggcctggg cgcgaccggc gatccggtgt tctgctcgat
     5101 ctggacgctg ttcggcatgc cggcgatcac gctgccgctg atgcagggcg agaacggcct
     5161 gccgctcggg gtgcagctcg tcggtcggaa ggggtacgac gcgcggctgt tgcgcaccgc
     5221 gcgctggctg gagaagaaag tcgcttctta gggcgcggcg gcgagcgcct tcaggccttc
     5281 gagctcggcg cgcagctttt ccacctcgct gcgatccgcg aaggcgacca cccagtgggt
     5341 gagctgaccg tcggcgccgt gcacggcgcc gaaggagatc tcgacttggc gcaccgcgcc
     5401 gtccttgccc caggcgcgga gcttccagcg cgagctcgat tccgcgagca ggcggtggac
     5461 cagcggctca tcgccgtgga acagcaaagc cgcgagcggc cggccgagcg cctcgccggc
     5521 gcggtagccg aagaaccctt cgaacgcggt gttgacgtag gtgaccggac gcgagccgat
     5581 gcgcgcatcg agcatcgcga gcggaagccc gcacgcgccg agcgccgcgc gcgaaagcgc
     5641 gctgtccgac agcaggcggt agagcgcagg cgccgtttcg ggcttgacga ttctctgctg
     5701 ggcgagcgcc atgtgccggt tgtgccacga tttcgcggcc cggacaatgt ccatttttgg
     5761 cgacgcccgg gatttccggc gcgcggtgtg acaaattcct cgtcaggccg cgcgccgggt
     5821 ggtcacggac agcgaatagc aggagcggcg caggcggctg aagtccgcgg tctcgatggt
     5881 ctcgcgcagg aaatccatgt tgcggtcgat ctcggactgg tacgggctcg ggccgtcgaa
     5941 gtgcgcctcg gcgtagcggt cgacgctacc gagcttcttc tggatgcggg tacgggcgac
     6001 gtactcgtag aatcccgggg tcttgatgat caggtcctcg ggcgaggcat agcgcaccag
     6061 ctcgtggccg tcgggcaggc gctcgcgcgc gatcttgccc cagacgaact ccttgtagag
     6121 gaactcgcgg catttctcga ggtacatgcg gtccgacatt tgcccgagca gatcggcggt
     6181 gccgaccagg cagccgatca cgcggtcctt gggatcctcg acccggatgt cgtcgatctc
     6241 catctcgtag ccggtgaaat gcaccaggcg ggccgcggtc gcggcctcgg ccccgaagcc
     6301 cagcttcggc aggtaggcgg aaatgaagtc ggctccgcgg ctgacgtgcg tcttggtaaa
     6361 caccgccccg ttctcgaccg cggtttcgga tgcacgcctg agatagcccg agtcgtgcag
     6421 cagcgcgatc agcacgccga gaatcgcgcg gcgcgggccg acgcggtccg cagtcgtgca
     6481 ggcgcgctcg tagccgtcga cgaggcgcgc cagggcgagc gtcatgtcga gcgtgtggcg
     6541 catgtcgtgg tagagcgtgt cgcaggccag gtagcccgga tagccgcctt cgaacagggc
     6601 cttgaagtcg gcgaaggcgc gctcgagcgg cgcgaactcc acgcccggat agcgcgcggc
     6661 gtagatcgca agaattgcat cgcggacccg catagggtcc tcgacgttga cgcgattcgt
     6721 cacgtcgttc tgatttagac ggtcctgcgt agcctccccc ctgaagcagt cgattattct
     6781 ctgccggggg actggaagca agggagtgcg gccggtaagc cgggttctgt ggccccggtc
     6841 ggtttgaccc ggcggggccg gcagccattc ctctcgactc cgggttaccc cggagctcta
     6901 gccacctacc cgcaagcttg gccgagccgg cctgaagtgg cgtgttgcac caccatgctt
     6961 gcctatttgg tgttgctccg gatggaggtt gccgcgtttc accccttagc tctttcgagc
     7021 taaggactcg tctctgtggc cctcttcgtc acgtcgccgt gccagggagt taccctgcat
     7081 cctgctctgc ggagcccgga ctttcctctg cgctttcgcg cagcggctgc ctggccgact
     7141 ccgcctgcga ttttaccgcc ggggggcgga aatagcgcgc gtcatcatag aactcggcgc
     7201 tctcgccgcc gggatgccag ccgggataac cgaaaatcgg cagcggcgaa aggatgcggg
     7261 gcgaatcgcc cggcgcgcgc cgctcgagcc agcggagcgc ggcggcgtcg ggatcggccg
     7321 ccgccggcac cacgatcgcc ttgcaggtga tcccgggccg gggctcgagc gcctgctcca
     7381 gcaccgcgtg cccgaacacc acgatgcgca tcgcggcctg gacctgcttg cggttctccc
     7441 agaacaggct tttccagcga aagctcttca gcagagaaac cagctccggg tcgtcgcatt
     7501 tcacgatcgc gccgccttcg tcaaagattg tgagcaggtc tcgcaacggg ccgcggcgcc
     7561 cgccctcgcg cgggatctcg gcggcgtgca gggcgttgat gcgcgctttg gtgcgcggga
     7621 aggcgagcca cgcgagcgcg ttgaagaaat cgtgcaggtt gtcgggccgg gtctcgacgc
     7681 gcccggtctc atgaaccttg atctcgtaat acgcgtcctt cgtgcccggg ggcacgaagc
     7741 gcagcggccg gcccgactcg gttttcaggt cttgggcttg agcgagcgca ttgaggcgct
     7801 cgagcgagcg ctcgcccgcg agccacggtc gaatgccgtc aaaaacgggg tgcgctagac
     7861 gagcttccag gggatcgttt ctccggcgcg cagcggcacg agctcttcgc cgccgaaggc
     7921 taggcgctcg ggcacgcgcc attcagcccg ctcgagggta atggtgtcgc ggttgagcgg
     7981 caggccatag aacccggcgc cgaaggcgct ggcgaagccc tccagcttgt cgagcgcgcc
     8041 ggcctcctcg aaggcggcgg catagagctc gatcgcggcg tgcgcggtgt agatgccggc
     8101 gcagccgcag gcgccctcct tggcgtcgcg cgcatgtgga gcgctgtcgg tgccgaggaa
     8161 gaatttcggg tttcccgagg tcgcggcttc caccagcgct tcacggtggg tttcgcgctt
     8221 gagcaccggc aggcagtagt ggtgcggccg gatgccgccg agaaagagcg cgttgcggtt
     8281 catgagcagg tgatgcgcgg tgatcgtcgc cgcgacgttc ggcccggtga cctcgacgta
     8341 ctgcgccgcc tcgcgcgtgg tgatgtgctc gagcaccacc ttgagccgcg ggaagcgctc
     8401 gagcatagga cccagcgttt cctcgatgaa cgcctgctcg cggtcgaaca cgtcgatcgc
     8461 cgggtcggtc gattcgccgt gcaccagcag cggcaggccg agctcctcca tcttctccag
     8521 cgcatggaag cagcgcgaca ggcgcgtcac gccctcgtcc gaatgcgtgg tcgcgcccgc
     8581 gggatagagc ttgacgccgt gcacgtgccc cgacagtttc gcgcgtgcga tctcctccgg
     8641 cggcgtgtcg tcggtcaggt agagcgtcat cagcggatcg aaactggcgc catccggaag
     8701 cgcgccgagg atgcgttcgc ggtaatcgag cgcctgtgcc gtggtgcgca ccgcgggctt
     8761 gaggttcggc atcacgattg cgcgtgcgaa gcgccgcgcc gagtccgcga tcaccgaggc
     8821 gagcgccgcg ccgtcgcgca ggtgcaggtg ccagtcgtcg gggcgccgga gcttcagacg
     8881 gtccatcaac ttaagatttt agagtcttac ggtagagcac gttctttggc acgcccgtga
     8941 tcttcgccgc gagccgcgct gcctcgctcg gcgccaccgc ttcgagcagg atctcaagga
     9001 cgttctccgg atcgatcccc ggttgcgcct cgcgcgcggt ccctggtgcc agcacaagca
     9061 cgaattcacc cttgctgcgt tccgcgcggc cggcgagcca tgccggcgca ccggagagcg
     9121 gcatgcgggc gacctcctcg aacttcttgg tgagctcgcg ggcaacgagg atttctctcg
     9181 catcaccgaa cttctgcctc aggtcctcca gcgtgtcagc gatccggtgc ggcgcctcat
     9241 agatgaccac cggccagggg aggtcgagcg catcgagcgc cttgcgccgc gccgcggcgc
     9301 ccgccggcag gaagccagcg aacagaaact gcggggcggc gaagcccgac accgacagcg
     9361 ccgccgcggc ggcgctcggg ccgggaaccg ggctgacgcg gatgccggcg cggtgcgctt
     9421 cggcgaccag ccacgcgccg gggtcggaga gcgccggcgt gccggcgtcg ctgaccagcg
     9481 cgatgctttt gccctcgcgc agcgccgcga tcagcgcggc ggcggcgcgg cgctcgttgt
     9541 gctggtgcaa ggcgacggtg cgggcggcga cgccgtggcg cgaaagcagg gtgcgggtcg
     9601 tccgcgtgtc ctcgcaggca acgatgtcgg cgcgcttcaa cgtatcgagc gagcgcgggc
     9661 tcgcatcgga gaggttcccg atgggcgtcg cgaccacgta taggactccc gaagtttgga
     9721 ctgccggcgt caaccgcgca ccctccagtt ccgttaagcc tgccatgaac gcggaggggg
     9781 cgcgagcgga ggatctctgt gcagggctgc tgcgcgcggc gggcctggtc ctgatcgagc
     9841 gcaactggcg ctgccgccac ggcgagatcg acctgatcgc cgaggagcgc ggcatgctgg
     9901 tgttcgccga ggtgcgcatg cgcagccgcc ccgggttcgg cggcgccggc gagagcgtga
     9961 ccgcggcaaa gcgccagcgc ctgctcgccg ccgcgcgcct ttatctcgcg cgccggccgg
    10021 aggcgctgtg ccgctttgac gttttcctgg tcgatggcgc gacttcgaac gtgcaatgga
    10081 tccgcgacgc ctttggcgaa taggcatcct tctcaccctg gcatgggggg atcctcatgc
    10141 ggaagagcgc gttcgcctgc tggtccagaa ctcgccgctc gccgggttcc gctatcacgc
    10201 cgccgccgag atgtggcgcg agctgcgggt cggcgacccg ctcgagctcg cccgcgagcc
    10261 cggcaacccg cacgacgcga acgcggtgag ggtgctgtgg cgcggccaca tgctcggcta
    10321 cgtgccgcgg cgcgagaacg gcgcgctggc gtggggcctg gaccgcggcg cgccgctgcg
    10381 cgcgcgcatc agcgcgctcg ccgatcaccc gaatccggcg cggcgcgtgc gcttcgaggt
    10441 ctacgtcgag taaaatccgc gccatggccc aggccgagat ttcgcagcgc atcgccgcgc
    10501 atttcgcgga aagcgcgaat ctcaagctcg ccgcggcgca ggcgctcacc gagccgctcg
    10561 agcgcgcgtc gcggctcatg gtcgacaccc tgcgcggcgg cggcaagatc ctctcgtgcg
    10621 gcaatggcgg ctccgcggcc gacgcgcagc acttcgccgc cgagctggtg aaccgcttcg
    10681 agagagagcg tccgccgctc gctgccatcg cgctcagcac cgacacttcg agcctcacct
    10741 cgatcgccaa cgactacgcc taccagcagg tcttctccaa gccgctgcgc gcctttggcc
    10801 ggcgcggcga cgcgcttctc gcgatctcga ccagcggcaa ctcggcgaac gtgctcgacg
    10861 cgatccatgc cgcgcacgag atcggcgtgc gcgtcgtggc gctcaccggc aacggcgggg
    10921 gcaagatggg cgcggcgctg cgcccggacg acgcgcatct ttgcgtgccg caccgcacga
    10981 ccgcccgaat ccaggaagtt catctccttt gcattcactg cctatgcgac gcaatcgata
    11041 ctttgttatt cccggtctga tcgccgtcct ttgcttgcag ggctgcccgg cgatcatcgc
    11101 cgggggcagc gtcgtcgccg tgacctcgct cgaggaccgc cgcaccacgg gcacccagct
    11161 cgacgacagc ggcatcgaat cgaacgccgc cagccgcatc ggcgagcgca tcggcgagcg
    11221 cgcgcacgtc aatgtcacgt ccttcaaccg cgccgtgctc ctcacggggg aagcctggga
    11281 tgaagccacg cgcgccgagc tcgagaagat cgtggctacg gtgccgaacg tccgcaacgt
    11341 gaccaacgaa gtccaggttg ccggcgcttc ctcggccacc tcgagagcca acgacaccgc
    11401 gatcacggca agagtgaaag gccgcttcgt caacgtcaag gacctgaacc cgctgcacgt
    11461 gaaggtggtc accgaggcgg gcgtggtctt tctactcggc ctggtcaccg agaccgaggc
    11521 cgcggccgcg accgacctcg cgcgcacgac cggcggcgtg cgcaaggtgg tcaaggtgtt
    11581 cgagtactgc aagagcacgg aagctccctg caagcccgcg ccccccgcgg ctccgcccaa
    11641 gtcgcagcgg tgaagccgcc cgagttcgaa gacaagatcc acgaccccgc cgcgctgcaa
    11701 aactggatcg gccggctcgc gcgcccgctg gtgttcacta acggcgtttt cgacctcctg
    11761 catcgggggc acgtcaccta tctcgcgcgg gcgcgcgccc tcggggccgc gctgctggtg
    11821 gccttgaatt cggacaagtc ggtcaggaac ctcggcaagg gaaacgatcg tcccctgaac
    11881 ccgctggcgg accggctggc ggtgctcgcg gcgctcgagt gcgtcgacgc ggtcacctgg
    11941 ttcgacgacg acacgccgga gcggttgatc gccgcgagcc ggccgcaggt gctcgccaag
    12001 ggcggcgact ggccggccga gcgcatcgtc ggcgcgggcg gcgtgctcgc tcgcggcggc
    12061 caggtggtat cgattccctt cgagcaccag cgctcgacca ccgacctggt gggacggatc
    12121 aggaaaggct gagctcgaag cagagcgcgc ccggtgtggg gttctccagg tagggcgcga
    12181 tcggcttgaa cccgagcatg ccgtacagcg cctgcgcttc gcgcatcttc ggcagcgtat
    12241 cgagcacgat gcgggtgcag ccggcttcgc gcgccgcggt gatcgccgct tcggccagca
    12301 cgcgtcccag accgcggccg cgctgcgcgg ctcgcacgta gagccgcttc atttcggcgg
    12361 tttgccgatc caggaaacgc accgcgacgc atcccgccgc atcgtccagc aagaacaatc
    12421 ggtactcgcc gggaagcgag tcgagctcgc gctcgaagcc cgtgaagcag cagggctcgt
    12481 cgactccgtg cgcgtactcg gcgaagagct cgcgcaccac tgccacctgc gcctcgccgg
    12541 tagcctccac cagcctggcc atataaactt cctccatgcg cctgcaccag ctcaaagtcg
    12601 attatgacgc cgagcaggac cggctactga tgctggtcgc gacgagcgag ggcgtcgagc
    12661 tcaggctgtc gctgacgcgg cgcttcgtga agctgttgtg gccgctgctc gtcaagcttg
    12721 ccgaggaagc gagcccgcgc atccgcaccc aggccaaccc cgaggcgcgc aaagcgctgc
    12781 tcggcctgga gcacgagcat gcggtatcga aggccgattt ttccaagccc tacgacgccg
    12841 ccggcagcga gcgcccgctc ggcgaggcgc cgctcctcct ggcgcgaatc cagaccggcc
    12901 atgaccgcaa cggccagccg gtggtagccc tgcaccctgc cgaaggccag ggaatcacgc
    12961 tcactttcga ctcggtcctg ctgcattcgg tgtgcaggct gctgcagtcg gcggtcaaga
    13021 agagcgactg ggacatggag ctcaagcttc ccggcaccga gccgcaagag ggcggcgagc
    13081 gcgtccgaac gctcaattga cttataattc aagattatcg cttcagcttt tgaccaactc
    13141 ggtttaattc ccccgctcgc cgcagccgtc gcggaactcg gttacaccac gcccacgccg
    13201 gtgcaggagc aggcgattcc gctcgtcctc ggcgggcgcg atctcatggc cggggcgcag
    13261 accggtaccg gcaagaccgc agcgttcgcg ctgccgatcc tgcagcgctt tgcgccgctc
    13321 gccagcgctt cgacttcgcc cgcgcgtcac ccggtccgcg cgctggtcct cacgccgacg
    13381 cgcgagctcg cggtgcaggt cgccgagagc ttccgcgact acggcaagaa cctgcccctg
    13441 cgcagcaccg tggtgtacgg cggcgtggac atgaacgctc agatcgagca gttgcgcaaa
    13501 ggggtggaga ttctggttgc gactcccggg cgcttgctcg accacgtgca gaacaagacc
    13561 gtgatgttca atcaggtgtc ggtgctgatg ctcgacgagg ccgaccgcat gctcgacatg
    13621 gggttcctgc ccgacatcaa gcgcatcatc gcgctgctgc cgcgggaaag gcagaacttg
    13681 ctcttctcgg cgaccttccc cgacgagatc cgcacgctgg cgaagaccct gctgcgcaac
    13741 ccggccgagg tccaggtggc ggcgcgcaac gccgcggcgg agaacgtcag ccacgtgctg
    13801 catccggtgg cgcgcgagaa gaagcgcgag cttctcgcct acctcatcca gactcgcggc
    13861 ttgcgccagg tgctggtgtt taccggcacg cgcatcggcg ccaaccgcct cgcccaccag
    13921 ttgcggcgcg atcacatcca cgccgacgcg atccacggcg acaagtcgca gatcgagcgc
    13981 gaagccgcgc tcgccgactt caagtcgggc aagactgcgg tgctggtcgc gaccgatgtc
    14041 gcttcgcgcg gcctggacat cgagggcctg ccgcaggtga tcaatttcga cgtgccgcac
    14101 tcccccgagg actacgtgca ccgcatcggc cgcaccggcc gtgccgggct tccgggcgag
    14161 gcgatctcgc tggtcgcgcc gcaggaccac gacgccatgg cggcgatcga gcggctgatc
    14221 aagaagcgca tcgagcgcca gctgatccca ggcttcgggc ctagcggcgc cacggtggcg
    14281 gcgatgatga gcgatcagcc ttcgcgcaag gaaaaggacc gtcgaccagc gccgagcacc
    14341 caggatcgtc gaccagcgcc gagcacgcag ggccggcgac aaacgccgag cacgccgccc
    14401 aaacgccaga tcgatccgat tttctccaag ccttacgaac cgcccgccgc agccagcccc
    14461 gcccccgccg caaaccaaga gcccccgcag caccccaggc gcaagcgtcc ccaggtcgcc
    14521 gccctgctgg gcggcatgaa gaaagcctcc tgactacacg cagcgcgcgc cgtcgacttc
    14581 gatgcagacg ccgctgatga actccgcctc gtcggacgca aggtaaaggc acgcgttcgc
    14641 gacgtcgagc ggcgtcgaga agcggccgag cgggacggtt gccaggaagc gcttcttcgc
    14701 ctcttcggtc actgcgccgc ccgcgaactc ggcggacagc gccgtgtccg ggctgaacac
    14761 cggattgacg cagttcaccc ggatgcggtc ggggccgaac tcgcccgcca tcgacttcga
    14821 catcaggatt accgcgcctt tcgagctgtt gtagaccgtc agcccggggc gcgggcgcag
    14881 gcccgcggtg gaggcgatgt tgacgaagca gccccgcccg cgcttgcgga acacgggcac
    14941 ggcgtgcagc gccgagaggt agatgctctt cacgttcacc gcgtagacgc ggtcgaactc
    15001 ggcctcggtg acttcgagat agggcttgtt gcgatgggtc cagccggcgt tgttgacgat
    15061 gatgtccagg cgctcgccgg ccgcgcccac gagcttgcgc cagtcgtcgc ccctggtcac
    15121 gtcggcttgg atgaatttcc cttccacttc gcgcgccacg cgctcgcccg cctgcgcgtt
    15181 gatgtcggcg acgatcacgc gcgcgccttc ctcggcgaag cgcttggcga tgccggcgcc
    15241 gaagccggac ccggcgccgg tcacgatggc agttttttcc ttgagtctca tggtttcttg
    15301 aacggactat gttcgcataa ttcgtcgaca taacgcgtga ccccggtgcc ttcgcgctcg
    15361 agaaagctct ccaccgcgcg cgcgaacttc gggtgcgcga gccagtgcgc cgaaggcgct
    15421 tccaccggca ggaggccgcg ccagagcttg tgctcgccct gcgcaccgcc ttcgaacacc
    15481 tgcagcccgc agcggatggc gtactcgatc gcctggtagt agcagcactc gaaatgcagc
    15541 agcggcacgt gctcgagcgc gccccagtag cgcccgtaga gcgtgctttc atccttgaag
    15601 aagaaagagg aagcgatggg gcggccgtcg cgctcggcga cgagcagcgc gaggttgccg
    15661 ggcagcgcgg cgccgaggcg caggaaaaag tccaggctga gataggggct cgagcgatgc
    15721 tcggcgtagg tcctgcggta gcagcggttg aagaactccc agtgccgccg ctcgatggcg
    15781 cttccttccc accaggagaa tgcaatgccc gcttcccgga tgcggttgcg ctcctggcgg
    15841 atcttcttgc gccgctcgcg cgtcatgctg ttcagaaatg cgtcgaagtc cgcgtagccc
    15901 gcgttgcgcc agtgaaactg caccgtacgc cgcagcagca tgccgcgccc gacgaggtgc
    15961 ggaatctctt cctcgagggg aaagaggatg tgcagcgacg aggtgttccg cgcgagctcg
    16021 agcgccgcct ccagcaatac ttccggcgaa ttgcccatga gccgcggccc gcgaaccggg
    16081 gtgaaaggga tcgcgctcag gagcttcggg tagtactcca ccccgtgacg ctcgtgcgcc
    16141 tccgcccacg cccagtcgaa cacgtattcg ccgtaagagt gcgacttggc gaacagcggc
    16201 atcgccgcga ccagcgcgcc ggcgcgccgc accagcagga actgcggcgc ccagccggag
    16261 cgggccgacg cgcagccggt ctccatgagc gcggacagga actcgtgccg cacgaacggc
    16321 tggctgccgg cgagggcgtt ccattccgcc gcatcgacgc tgccgagcga atcgacgacg
    16381 cggacgcttt ccaaggttct acttgggctg cacgccgagc gccatacggc tgattctacg
    16441 ctgtacaatg cagccaaccg gaggccgggg gacggacaag gggatggaga cgcgcgcatg
    16501 aaaaagatcg aagcgatcgt gaagccgttc aagctcgacg aggtgcgcga ggcgctttcg
    16561 gaagtcggcg tcaccggcct caccgtgacg gaagtcaagg gcttcggccg gcagaaaggc
    16621 cataccgagc tctatcgcgg cgccgagtac gtggtcgact tcctgcccaa ggtcaagatc
    16681 gaggtcgtgg tcgccaacaa ccaggtggaa agcgcgatcg acgcgatcgt ccggtccgcg
    16741 cgcaccggca agatcggcga cggcaagatc ttcgtcacct cggtcgagca cgtggtgcgc
    16801 atccgcaccg gcgagaccaa cgaagccgcc gtctagcttc tgaggagtac caggaccgcg
    16861 ccggcaccgc cgtccgcggt gggggcttgg gaaaaagcca ggacctcgtc ccgcagtttc
    16921 agccagtggt agacctttcc cttcaggacc ggctcgcggt tacgcgagcc cagccccttg
    16981 ccgtgaacga tgcgcacgca gcccagccgc cgctcccggc agccgtgcag gaactcgcgc
    17041 accgcgcctg ccgcctcgct gcggttcatg ccgtgcaggt ccaggctgtc ctcgaccacc
    17101 caccgcccgc ggcgcagctt gcgcagcaag gcgggcgagt agcccgggcg cacgaaagcc
    17161 agctccgcgc ccgattcggt ggtgtcttcg ggcgcgatgg tttcgcgcgg gagcggcgcc
    17221 ggcctcgggc gctcgcgcac ggcgcgacgc gctttcacga gcggcaccac ccctgcgagc
    17281 gccttgcgaa agtcgctatc catgtccgtc caggtacttc tctgcgtcca gggctgccat
    17341 gcagccgcta ccggcgctgg tcaccgcctg gcggtagacg tgatcctgga cgtcgccggc
    17401 cgcgaatacc ccggggatgc tggtcgcggt ggccaagccc tcgcgccccc cgcgagtgat
    17461 gatgtagccg tccttcatct cgagctgccc cgcaaagatg ccggtgttgg gagtgtgccc
    17521 aatcgccacg aagaagccct ttgcggcgat cgtcttgacc gcgccacctt tcaaatttct
    17581 gacgcgaacc ccgctgaccc cgcccgagtc gcccagcacc tcctcgacga ccgcgtccag
    17641 ctcgaggcgg atcttgccct ccgcgacgcg cttcatcagc ttgtcgacca tgatcttttc
    17701 ggcgcggaac ttgtcgcggc ggtggatcag aaccacgctg gagcagagat tggcgaggta
    17761 gagcgcttcc tcgaccgcgg tgttgccgcc gccgaccacg accacgtcct ggcccttgaa
    17821 gaagaaaccg tcgcaggtcg cgcaggccga cacgccgcgc cccatgaact tctgctccga
    17881 gggaaggccg aggtacttcg ccgtggcccc ggtggcgata accagcgcgt cgcaggtgta
    17941 gcgcgccgtg tcgccctcga ggacgaacgg gcgctcccgc aggcgcgcgg tatggatctg
    18001 gtcggagacc acctccgttt cgaagcgctc ggcgtgcttc tggaaacgct gcatcaggtc
    18061 ggggccctgg acgccggcgg cgtcggccgg ccagttgtcg acgtcggtcg tggtcatgag
    18121 ctggccgccc tgcgcgaccc cggcgatcag caccgggcgc agattcgccc gcgccgcgta
    18181 gaccgcggcg gtataacccg ccgggccgga gccgagaatc agcaggcggg cgtttcgtgt
    18241 gtccatggac aaaatgctaa catcccgaat atggttagca cggtctccac cgcagtccta
    18301 aaaacggttc cgctgttcac gacctttccg gaagatcagc tgcggatgct gacgacgatg
    18361 gtgacgcgca agagcgcgcc gcgttcgacc accatcatgg cgggcggcga cgcgaccgat
    18421 tcgctctaca tcgtgctctc cggccggctc aaggtgatga tgagcgactc ggagggcaag
    18481 gaagtgatcc tcaccatcct cggcccgggc gagttcttcg gcgagatggg cctgatcgac
    18541 gacgagccgc gctccgccac ggtgatgacc atcgagccgt gcgagctgct ctccatcgcg
    18601 aagcgcgatt tcaagaagtg cctctcggag aactccgaga tggcgatggc cgtgatgcgc
    18661 agcctggtga agcggctgcg cgaggccgac cgcaagatcg gcagcctcgc cctgctcgac
    18721 gtctacggcc gggtggcgcg gctgctgctc gacatggccg agaacgtcga cggcgagaag
    18781 gtggtcacca agcgcctgcc gaagcaggac attgccaaga tgatcggcgc ctcgcgcgag
    18841 atggtgagcc gggtcatgaa ggacctgcag accggcggct acatcgagat gcgcggctcg
    18901 accatcgtgc tgcgcgacac gatcctgctc ccggagtaag cgggacaacg agggtcacct
    18961 cctggccgta gcaagcaaag gcgcgcccct tcccgccaag ctggcgggcc tgctgcgcga
    19021 agcgaaatgg ctgctcctca tcgccctcgc gctgtacctg ctcctcatcc tcgccacgtt
    19081 tcatcgcgcc gacccgggct ggtcgcatag cgcgaccgac gctgtcgcgc gcaacgccgg
    19141 cggcgcgttc ggcgcctggc ttgccgacct cctgctctac ctgttcggcg tttccgccta
    19201 ctggtgggcg gcgctatgcg cgtacctggt ggtctggggg tatcgccgga tggacggcgt
    19261 gaggctcgcc ggcgccgtgg cgggtttttc gctccttctt ctgtcgagtg ccgcgctcga
    19321 gcgcctgcgg ctccatagcc tcggcgccga gctgccgctc gccccgggcg ggctgatcgg
    19381 cgaagccctg gccggcgctt tttccggcgc gctcggcttt accggcgcca cgctcgccct
    19441 gctgacggct gccgcgatcg gcttcagcct gttcatcggg gtctcctggc tcaccctgtt
    19501 cgaaggggtc gggcgcctgc tggaaaaggc ctacgccgcg gcgctcggcg cgtgggagcg
    19561 ccgccgcgac cgcaagctcg gcagccgcgc gcgcgagcag cgcgaggcgg tagtcgaaag
    19621 cgggaagcgc cgcgaggaag agcatccgcc cctgcgcatc gagccagcgc tcgccgagat
    19681 caagaagtcc gagcgcgcgc agaaggagaa gcaggcgccg cttttcgagc acctgcccga
    19741 cacgccgctg ccgccgctca agctgctcga cgaggccgcg accggcggag aggcgatcgc
    19801 cgccgagacg ctggagttca cctcgcggct gatcgagaag aagctgtccg acttcggggt
    19861 cacggtcaag gtgctggcgg cgtaccccgg cccggtgatc acgcgctacg agatcgagcc
    19921 cgcggtaggg gtgaagggca gccagatcgt gaacctggtg aaggacctgg cgcgcgcgct
    19981 gtcggtggtc tcgatccgcg tggtcgagac catcccgggc aagtcgtgca tgggcctcga
    20041 gatcccgaat ccgcaccgcc agatcgtgcg cctgaccgag atcctcggct ccgaggccta
    20101 ccacgggatg cattcgccgc tcgcgctcgc gctcggcaag gacatcgccg ggcgcccggt
    20161 cgtcgccgac ctcgcgcgca tgccgcacct cctggtcgcc gggacgaccg gctcgggcaa
    20221 gtcggtcgcg ctgaacgcga tgatcctgtc gctcctctac aagtccgagc tgcgcgacgt
    20281 gcgcctcatc ctgatcgacc cgaagatgct cgagctctcg gtctaccagg acatcccgca
    20341 tctgctcgcg ccggtcgtca tcgacatgaa gcaggcggtg aatgcgctca cgtggtgcgt
    20401 ggccgagatg gaccgccgct acaagctcat gaactggatc ggcgcgcgca acctttcggg
    20461 ctacaaccac cgcgtggccg agcaggagtt cgaggacccc tttgctctag acgcgggcaa
    20521 ggcggagaag ctgacgccgc tgccgcaagt ggtggtggtg atcgacgagc tggccgacct
    20581 gatgatggtg gtcggcaaga gagtggagga gctgatcgcg cggctggcgc agaaggcgcg
    20641 cgcggccggc atccacctga tcctcgcgac gcagcggccg tcggtcgacg tgatcaccgg
    20701 cctgatcaag gccaacatcc ccacgcgcat cgccttccag gtttcgtcaa aagtggactc
    20761 ccgcacgatc ctcgaccagt cgggcgcgga agcgctgctc ggttcgggcg acatgctgta
    20821 cctgcctc
//
LOCUS       DATBSZ010000183        11770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1669901, whole genome shotgun sequence.
ACCESSION   DATBSZ010000183 DATBSZ010000000
VERSION     DATBSZ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11770
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669901"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1216
                     /locus_tag="VIG70_11865"
     CDS             176..1216
                     /locus_tag="VIG70_11865"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY8251295.1"
                     /translation="MLAKLAEELPPAGGYLYEPKWDGFRALVFRGADDVYIQSRDSRP
                     LDRYFPELHDALLERLPKGCVVDGEIVIVTAHGLDFDRLQLRLHPAESRVAKLAKETP
                     SSFVAFDLLAAGGRNLMAATQGERRAALEELLRKVKPPVYLTPVTKDRALAVDWLTRF
                     EGAGLDGVIAKPESSPYLPGKRAMIKVKHARTADCVVAGFRWHKSGKDAVGSLLLGLY
                     DDQGVLQHVGVTSSFTMAMRKQLARELEPLRREAIKDHPWKDWAGAAAESNRMPGAQS
                     RWSAGKDLSWEPLRVERVCEVKYDHMQGNRFRHAAIFLRWRPDKRPADCRYDQLEVTA
                     PYELDRIFRRDG"
     gene            complement(1180..2190)
                     /gene="ligD"
                     /locus_tag="VIG70_11870"
     CDS             complement(1180..2190)
                     /gene="ligD"
                     /locus_tag="VIG70_11870"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY8251296.1"
                     /translation="MAKKAETEVLVAAGREVAISNPAKVLFPKPKHTKLDLAQYYVSI
                     SDGALRGAGGRPNMLVRYPDGIQGEFFYQKRAPKARPEWIEVAELRFPSGRAAEEVVP
                     RDAAALAWMANLACLELHPHPVRAEDLEHPDELRVDLDPVPGVKWAQVRKIARVAEAA
                     LKDFGLAGWPKTSGKRGMHIYARIAPRWTFDEVRRAALALAREVERRAPQLATSKWWK
                     EERHGVFVDYNQNAKDRTIASAYSVRPTPDARVSAPLAWDEVDDCDPGDFTLASMPAR
                     FKQLGDRHAGIDERAGSLEPLLELSEKQNLGDAPWPPHYRKQAHEPPRVQPSRRKIRS
                     SS"
     gene            complement(2450..2992)
                     /gene="rnhB"
                     /locus_tag="VIG70_11875"
                     /pseudo
     CDS             complement(2450..2992)
                     /gene="rnhB"
                     /locus_tag="VIG70_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
     gene            complement(2985..4133)
                     /gene="lpxB"
                     /locus_tag="VIG70_11880"
     CDS             complement(2985..4133)
                     /gene="lpxB"
                     /locus_tag="VIG70_11880"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY8251297.1"
                     /translation="MADLLRVAMVAGEASGDQLAAPLMAALKARRNPIMFAGIGGPRM
                     EALGFESHYPMEKLSVRGYAEALMHYREIMGIRRRIARSLLAERPDLFIGVDASDFNL
                     DLERKLKDAGIPTIHYVSPSVWAWRAWRVRRIARSVTRILVMFPFEAPLYEKAGVPVT
                     YVGHPLADLIPLEPNKLEARAALRLPAGKLIVALLPGSRRSELQYMASSFVLAAHRFR
                     QEVHDVHFVCPTVTRDTRDMFERAVHEHQRTDLPLTLLFGHSHEALAAADLALVASGT
                     ATLETALFKTPMVIAYRQAPITWALMRAMIYLPYVGMPNVLAGEKLVPELLQDEANPA
                     ALAGALLTLLRDTAAQRRQIERFHEFHHLLRQNAAEKAADAILEVLHK"
     gene            complement(4133..4903)
                     /gene="lpxA"
                     /locus_tag="VIG70_11885"
     CDS             complement(4133..4903)
                     /gene="lpxA"
                     /locus_tag="VIG70_11885"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY8251298.1"
                     /translation="MIHPTALVHRGARLAADVEVGPYSVIGEHVEVGEGSWIGAHVVL
                     DGHTRLGRRNRIWHFSSIGAPPQDKKYAGEPTTVEIGDGNTIREYVTINRGTSQDAGA
                     TRVGNDNWIMAYVHFAHDCQVGSNTIFANLTGLAGHVTVGDWAIFGANTLVHQHVHIG
                     AHSFTGMATYLAQDLPPYVTAAGNMATPYGINKEGLKRRGFSPETIAGLKRAYKTLYR
                     KGLALEDALRELQGQVAECPEVQHILDFIARSKRGIIR"
     gene            complement(4912..5367)
                     /gene="fabZ"
                     /locus_tag="VIG70_11890"
     CDS             complement(4912..5367)
                     /gene="fabZ"
                     /locus_tag="VIG70_11890"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY8251299.1"
                     /translation="MAEIDIQKIFKFLPQRYPVLMIDRVKEIEPGRRIVALKNVSANE
                     PHFQGHFPGRPIMPGVLILEAMAQAAGVLVFSAEDMAEKRDRLLYYYVGIDNARFKKP
                     VVPGDQLEIDVTIERTLRGIGKFTCLARVAGAVVAEATILCSIRPVNDK"
     gene            complement(5360..6313)
                     /gene="lpxD"
                     /locus_tag="VIG70_11895"
     CDS             complement(5360..6313)
                     /gene="lpxD"
                     /locus_tag="VIG70_11895"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY8251300.1"
                     /translation="MPGPFTLGQIVSRLGGRVAGDPQTVVRQVGSLERAAADQITFFN
                     SRKLHTKLDQTRAAAVILAPENEALTRRPRIVAENPYAYFARVSQLFNAVVSQAPGVH
                     PSAVVAPSAKLGKGVSIGAGCVIGDDVSIGDDSCLYPRVVVYPKCVLGKRVVAHSGVV
                     IGADGFGIAREQERWIKIPQIGGVRIGDDVEIGANTTIDRGALDDTVIEEGVKLDNQV
                     QVGHNVRIGAHSAVAGCVGIAGSADIGRHCTIGGAAMIHGHITIADHVHVSAGTLISR
                     SLLKPGTYTGFYPFDEHEAWKRNAVQLRRLGKVKSRGKKNG"
     gene            complement(6319..6852)
                     /locus_tag="VIG70_11900"
     CDS             complement(6319..6852)
                     /locus_tag="VIG70_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY8251301.1"
                     /translation="MKNILAAAVLATLAVSAAGQAPAPQASAGGSKFGFVHTERILRD
                     SIPAVRAQKKIEAEFQKRDRELAGIADQLKKMQTELDKDAMTMGETQRRAKEREFGEL
                     NREFQRKQREFREDLNQRRNEELAQVVEQANRIIRMIAEQEKFDIIFQEMPAYVSPRI
                     DITDKVIKALEGKPPAK"
     gene            complement(6853..9150)
                     /gene="bamA"
                     /locus_tag="VIG70_11905"
     CDS             complement(6853..9150)
                     /gene="bamA"
                     /locus_tag="VIG70_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY8251302.1"
                     /translation="MLRNTGVFAAVFALVLAVAAALVPNPAAAQEFQPFVVKDIRVEG
                     LQRTEPGTVFSYLPVKVGETMNADKARAALRALYATGFFADVRLDVENDVLVVFVRER
                     PAIAQIDFSGMKEFEPDNVRKVLRELGMAEGRIFDRALLDSAEQELKRQYLSKGMYAA
                     AVQTTVTPLERNRVGISIAVTEGPVAKIRGINIVGAQAFTESELLGLFVLRTPGWLTW
                     YTQADRYSRERLGADLETLRSFYLNRGYLDFALESTQVAITPDRRDIYITINMSEGET
                     YTVSEVSLAGQLLLPREELETLIQLKRGDVFSREKLAASTKAISDRLGNEGYAFANAN
                     AIPNIDKEKRAVAFSIVIDPGRRVYVRRIDVAGNAKTRDEVVRREMRQLEGAYYDASK
                     IQLSRRRIDRTAFFSEVTVETLPVEASPDQVDVLYTVKEKATGSLLLGVGFSSVERIA
                     LSASVSQANVFGTGKYISANINSGSVNQVYSLSYMDPYYTVDGVSRGFDIYRRVTDAS
                     SLDTGAYKTDSLGGGIKFGYPVSEQISVDFGAAIESVDLEIFATSPLAYIRFVRDFGT
                     QYTYGSGTAGWTRDTRDSLITTTAGSFLRASGEIAGGDLQYYRLGYQQQWYYPMTRTF
                     TLHLGGEIGWAGGYGEQPLPFFKNYYAGGTGSVRGFRSFSLGPKDENGDATGGNRKLT
                     GVAEFLFPMPGAEQDRSLRLAAFLDAGQVYAGNIVLGELRYAAGIALFWSSPLGPLRL
                     SWAHPINVQSGDRKQQLQFTFGTGF"
     gene            complement(9288..10652)
                     /gene="rseP"
                     /locus_tag="VIG70_11910"
     CDS             complement(9288..10652)
                     /gene="rseP"
                     /locus_tag="VIG70_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY8251303.1"
                     /translation="MSLIHTIVAFIVALGLLIVVHEYGHYLIARLCGVKVLRFSVGFG
                     RPLLTREIGADRTEWVIAAIPFGGYVKMLDEREGPVEPREVHRAFNRQSVWRRFGIVV
                     AGPVANFLFAIAAYAGLFMYGLPEARPVLGTPPEGTVAAAAGLRSGDTVRSVEGELIT
                     TWQELRWRVLQSALQRSPLRLEIVNDRGHLNAVTLDLRSFPTDDVESDALERIGLRLY
                     RPPLEPVIGQVVRGGAAEKAGLAPGDRVLLADGRPVESWEALVAAVQARPEMPLALTI
                     ERAGARQAVEVVPAAVAAGPKRIGRIGAAPQVPASHAEKMLIRVQHGLGESLWKSIVK
                     TGDIAVFSLKMLGKMLVGEVSWKHLSGPVTIADFAGQSAQMGWVSYLTFLALISISLG
                     VLNLLPIPLLDGGHLMYYAIEIVKGKPVSERAMELGQRVGLALLLVMMAFAFYNDLNR
                     LLTG"
     gene            complement(10786..>11770)
                     /gene="dxr"
                     /locus_tag="VIG70_11915"
     CDS             complement(10786..>11770)
                     /gene="dxr"
                     /locus_tag="VIG70_11915"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY8251304.1"
                     /translation="AVGEDRALKRRFSEFDTELLFGPEALEVVARHPETQTVMAAIVG
                     AAGLASTLAAVRAGKRVLLANKEALVMAGPLVMHAASESGAQLLPVDSEHNAVFQCLG
                     SARNPRRIILTASGGPFRAAAPESLSQVTPKEACAHPNWVMGRKISVDSATMMNKGLE
                     VIEARWLFDLAPERIEVLIHPQSVVHALVEYADGSVIAQLSNPDMRVPIANALAYPER
                     LESGAQSLDLASLKTLSFEKPDLVRFPCLGLAYAALRAGGTAPAILNAANEIAVEAFL
                     AGRLAFTGIAGVIADTLAALPAGAAGDLAEVMEADAQARKAARLRVLALAA"
BASE COUNT         1840 a         3893 c         4075 g         1962 t
ORIGIN      
        1 tcatgggact actgatgatg aatcgcaaat ggcttaggta gttggcgttc gagccgccag
       61 attagccaga aggaattcgg caagtttttg atttgcctag cttgtacaaa tggccgcacg
      121 aaaaccaagc agtgatgctg ctccggagct gccggtagtc gcgccgattg agccgatgct
      181 cgcgaagctt gccgaggagc tgccgccggc gggcggatat ctgtacgagc cgaagtggga
      241 cgggtttcgc gccctcgtgt ttcgcggcgc ggacgacgtg tacatccaga gccgcgattc
      301 gcggccgctg gatcgatatt tccccgagct gcacgacgcg ctgctggagc ggttgccgaa
      361 gggctgcgtg gtggacggcg agatcgtgat cgttaccgcg cacgggctcg acttcgaccg
      421 gctgcagctg aggcttcatc cggcggaatc gcgtgtcgcg aagctggcga aagagacgcc
      481 gtcgtcgttt gtcgccttcg atctgctcgc cgccggcggg cgcaatctca tggctgcgac
      541 gcagggcgag cggcgcgcgg cacttgagga gctgctccgg aaggtgaagc cgcctgtcta
      601 tctgacgccg gtcacgaagg atcgggccct cgcggtggac tggctgacgc gcttcgaggg
      661 cgccgggctg gacggcgtga tcgccaagcc cgagtcgagc ccgtacctcc cggggaagcg
      721 cgccatgatc aaggtgaagc acgcgcgcac ggccgactgc gtggttgccg ggtttcgctg
      781 gcacaagagc ggcaaggatg cggtggggtc actgctcctc ggcctgtacg atgaccaggg
      841 tgtcctgcag cacgtaggcg taacttcctc cttcaccatg gccatgcgaa agcagctagc
      901 ccgggagctg gaacctctgc gcagggaagc gatcaaggat cacccctgga aggactgggc
      961 gggcgctgcg gcggaatcga atcgcatgcc tggcgcgcag agccgctgga gcgcggggaa
     1021 ggacctgtcg tgggagccgc tgcgcgtgga gcgcgtgtgc gaggtcaagt acgaccatat
     1081 gcagggcaat cgtttccgcc acgcggcgat cttcctgcgc tggcggcccg acaagcggcc
     1141 ggcggactgc cgctacgacc agctcgaggt caccgcgccc tacgagctcg accggatctt
     1201 tcgccgcgac ggctgaacgc gcggcggctc gtgcgcctgt ttgcggtagt gcggcggcca
     1261 cggcgcgtcg cccaggttct gcttctcgga caattccaat agcggctcga gcgagccggc
     1321 gcgctcgtcg atgccggcat ggcggtcgcc gagctgcttg aagcgcgcgg gcatcgatgc
     1381 gagggtgaag tcgccgggat cgcagtcgtc gacctcgtcc caggcgagcg gtgccgagac
     1441 gcgcgcgtcg ggggtgggcc gcaccgagta cgccgaagca atcgtgcggt ccttggcgtt
     1501 ctggttgtag tcgacgaata cgccgtggcg ctcctccttc caccatttgc tggtggcaag
     1561 ctgcggcgcg cggcgctcga cttcgcgcgc gagcgcgagc gctgcgcggc gcacttcgtc
     1621 gaaggtccag cgcggagcga tgcgcgcgta gatgtgcatg ccccgcttgc cggacgtctt
     1681 cggccagcca gcgaggccga agtccttcag cgctgcttcg gccacgcggg cgattttgcg
     1741 aacctgcgcc cacttcaccc cgggcacggg gtcgaggtcg acgcgcagct cgtcgggatg
     1801 ctcgaggtct tcggcgcgca ccggatgcgg gtgcagctcg aggcaggcca ggttcgccat
     1861 ccaggcgaga gctgctgcat cgcgcggcac cacctcctcc gccgcacgtc ccgagggaaa
     1921 gcgcaattcc gcgacctcga tccactccgg gcgcgccttc ggcgcgcgct tctgatagaa
     1981 gaactcgccc tggatgccgt cgggatagcg caccagcatg ttgggccggc cacccgcacc
     2041 ccgaagtgct ccgtctgata ttgaaacgta atactgcgcg aggtcgagct tggtgtgctt
     2101 cggcttcggg aacaggacct tggccgggtt cgagatcgcg acctcgcgcc cggcggcgac
     2161 caggacttca gtttcggctt ttttcgccac tgcggagatc cgcgcgagcg ttccgcaaca
     2221 aggtcctgac caggtcgata tggggggcgg cgtcacgcga agcgaacacg tcggcgtact
     2281 cgcgcctgag gcgcttcgcg cggaaatcga agtcggcgag gcgtttcact gcgctcgaaa
     2341 gcttcgtcgc ctcgcagtac agccggtagc tgcggatgcg cagcgattcg gccatggtct
     2401 cccaggtgga gccgaacagg ttgccctgga agaaaacccc gaccgcgtag aagctccggc
     2461 ggtggatttc gcagggcccg agccgcccga ccgcctcgag gtgctcggcg gtggcgtagc
     2521 ccttgtgctg gtcgaagccg tattgcggga agcggtcgtg cagcgcgcgc atttcggcgt
     2581 cgcgcgcggt cttggccagg atcgaggctg ccgagatcgg cttgtgcgtc gcatcgccgt
     2641 cgacgatggc gcgcgccggg caaggcaggt cggggcacat gttgccgtcg atccacgctt
     2701 catgcggggg gatcgcgagc aactcgacgg cacggcgcat ggcgaggagc gaggcgcgca
     2761 ggatgttgat cctgtcgatc tcctccacgc tcgcgtgcgc cacggcccag gcgatggcgc
     2821 gctccttgat ccggccggcg agcacctcgc gccgctccgg ggtgagcacc ttggagtcgg
     2881 cgaggccgtt gacgcgcctg gcggggtcga ggatgaccgc ggcggcgtac accggcccgg
     2941 cgatcggccc gcgccccgct tcatcaacac cgcaagtcag gatgctattt atgcaaaacc
     3001 tccaagatcg cgtcggcggc cttctccgcc gcgttctgcc gcaggaggtg gtgaaactcg
     3061 tggaagcgct cgatctgccg tcgctgggcc gcggtatcgc gcagcagcgt cagcagtgcg
     3121 ccggcgagcg cggccgggtt cgcttcgtcc tgcagcagct ccggcaccag cttctcgccc
     3181 gccagcacgt tcggcatgcc gacgtagggc aggtagatca tggcgcgcat cagcgcccat
     3241 gttatcggag cctggcgata cgcgatcacc atcggcgtct tgaagagcgc cgtctcgagg
     3301 gtcgcggtgc cgctcgccac cagggcgagg tcggcggcgg cgagcgcctc gtgggaatgg
     3361 ccgaaaagga gcgtcagggg caggtcggtg cgctggtgct cgtgcaccgc gcgctcgaac
     3421 atgtcgcgcg tgtcgcgcgt gacggtcggg cagacgaagt gcacgtcgtg cacctcctgc
     3481 cggaagcggt gcgccgcgag cacgaacgag ctcgccatgt actggagctc cgagcgccgg
     3541 ctgccgggca gcagcgcgac gatcagcttc cccgccggaa ggcgcagcgc cgcgcgcgcc
     3601 tcgagcttgt tcggctcgag cgggatgagg tcggcgagcg ggtggccgac gtaggtgacc
     3661 ggcacgccgg ccttttcgta gagcggcgcc tcgaacggaa acatcaccag gatgcgcgtg
     3721 accgagcgcg cgatccggcg cacgcgccag gcgcgccagg cccagaccga agggctcacg
     3781 taatggatcg tggggatgcc ggcatccttc agcttgcgct ccaggtccag gttgaagtcc
     3841 gaggcgtcga cgccgatgaa gaggtcgggc cgctcggcga gcagcgaccg ggcgatgcgg
     3901 cggcggatcc ccatgatctc gcggtagtgc atgagcgcct cggcgtagcc gcgcaccgag
     3961 agcttctcca tcgggtagtg cgactcgaag ccgagcgcct ccatgcgcgg cccgccgatg
     4021 ccggcgaaca tgatcgggtt gcgccgcgcc ttcaacgcgg ccatcagcgg cgccgcgagc
     4081 tgatcgcccg acgcttcgcc cgcgaccatg gcgacgcgga ggaggtctgc cactaccgga
     4141 tgattccgcg cttgctgcgg gcgatgaaat ccaggatgtg ctgcacttcg gggcactcgg
     4201 ccacctgccc ttgcagctct cggagggcgt cctcgagagc caggcccttg cggtagaggg
     4261 tcttgtaggc gcgcttcagc ccggcgatcg tctccggaga aaagccccgt cgcttcaggc
     4321 cttccttgtt gatgccgtag ggcgtggcca tgttgccggc ggcggtgacg tagggcggta
     4381 ggtcctgcgc caggtaggtg gccatgccgg taaacgaatg cgcgccgatg tgtacgtgct
     4441 ggtgcaccag cgtgttggcg ccgaagatcg cccagtcgcc gaccgtgacg tggccggcga
     4501 gcccggtgag attcgcgaag atggtgttac tgccgacctg gcagtcgtgc gcgaaatgca
     4561 cgtaggccat gatccagttg tcgttgccga cccgcgtggc gccggcgtcc tggctagtgc
     4621 cgcggttgat ggtgacgtac tcgcggatgg tgttgccgtc gccgatctcg accgtggtcg
     4681 gctcgccggc gtacttcttg tcctgcggcg gcgcgccgat cgaggagaag tgccagatgc
     4741 gattgcgccg gccgaggcgc gtatggccgt cgagcacgac atgggcgccg atccagctgc
     4801 cctcgcccac ctctacgtgc tcgccgatca ccgaataggg gccgacctcg acgtcggcgg
     4861 cgaggcgcgc gccgcgatgc acgagtgcgg tcggatggat catcgtgcgc cctacttgtc
     4921 gttcaccggc cggatcgagc acaggatggt ggcttcggcg acgaccgcgc cggcgacccg
     4981 ggccaggcag gtgaacttgc cgatgccgcg cagcgtgcgc tcgatcgtca cgtcgatctc
     5041 gagctggtcg ccgggcacta ccggcttctt gaagcgggcg ttgtcgatgc cgacgtagta
     5101 gtagagcagc cggtcgcgct tctcggccat gtcttcggcg ctgaacacga ggaccccggc
     5161 ggcctgcgcc atcgcctcga ggatcagcac cccgggcatg atcggccggc ccggaaagtg
     5221 gccctggaaa tgcggctcgt tcgcggagac gttcttcagc gcgacgatgc gcctgccggg
     5281 ctcgatctcc ttcacgcgat cgatcatcaa gaccggatag cgctgcggca ggaacttgaa
     5341 aatcttctgg atatcgatct cagccattct ttttccccct gctcttgacc ttgcccagcc
     5401 ggcgaagctg cacggcgttg cgcttccagg cctcgtgctc gtcgaacgga taaaagccgg
     5461 tgtacgtgcc cggcttgaga agcgagcgcg agatcagcgt cccggccgag acatgcacat
     5521 ggtcggcgat ggtgatgtgg ccgtggatca tggcggcgcc gccgatggtg cagtgccggc
     5581 cgatgtcggc gctgccggcg atgccgacgc accccgcgac cgcgctatgc gcgccgatgc
     5641 gcacgttgtg gcccacctgc acctggttgt ccagcttgac gccctcctcg atcaccgtgt
     5701 cgtcgagcgc gccgcggtcg atcgtggtgt tggcgccgat ctcgacgtcg tcgccgatgc
     5761 gcacgccccc gatctgcggg atcttgatcc agcgttcctg ctcgcgcgcg atgccgaagc
     5821 cgtcggcgcc gatcacgacg ccggagtggg ccacgaccct ttttccaaga acacattttg
     5881 gatagaccac cacgcgcggg tacaggcagc tgtcgtcgcc gatgctaacg tcatcgccga
     5941 tcacgcagcc ggcgccgatg gagacgcctt tgccgagctt cgcgctcgga gcgaccacgg
     6001 cggaaggatg cacgcccggc gcctggctga ccaccgcgtt gaacagctgc gagacacgcg
     6061 cgaagtacgc gtagggattc tcggcgacga tgcgcggccg ccgggtgagc gcctcgttct
     6121 cgggcgccag gatgaccgcg gcagcgcgcg tctggtccag cttcgtgtgc agttttctcg
     6181 agttgaaaaa agtgatctgg tctgccgccg cgcgctcgag cgagccgacc tggcgcacca
     6241 ccgtctgcgg gtcgccggcg acgcggccgc cgagccgcga aacgatctgc ccgagcgtga
     6301 acggccccgg catcgggtct atttggcggg cggcttgcct tccagcgcct tgatcacctt
     6361 gtcggtgatg tcgatgcgcg ggctcacgta ggccggcatc tcctggaaga tgatgtcgaa
     6421 cttctcctgc tccgcgatca tgcggatgat gcggttcgcc tgctccacca cctgcgccag
     6481 ctcctcgttg cggcgctggt tgagatcctc gcggaactcg cgctgctttc tctggaactc
     6541 gcggttcagc tcgccgaact cccgctcctt ggcgcggcgc tgcgtctcgc ccatcgtcat
     6601 cgcgtccttg tcgagctcgg tctgcatttt cttcagctgg tcggcgatgc cggcgagctc
     6661 gcggtcgcgc ttctggaact cggcctcgat cttcttctgc gcccgcaccg ccgggatcga
     6721 gtcgcgcagg atgcgctcgg tgtgcacgaa gccgaacttg ctgccgccgg cggaggcttg
     6781 aggggcaggc gcctgccccg ccgcggatac cgccagcgtc gccaggactg cggccgcgag
     6841 gatgttcttc attcaaaacc ctgttccgaa ggtgaattgt aactgctgct tccggtcccc
     6901 gctttgaacg ttgatgggat gcgcccagga gaggcgcagc ggcccgagcg gcgaggacca
     6961 gaacagcgcg atcccggccg cgtagcgcag ctcaccgagc acgatgttgc cggcatagac
     7021 ctggccggcg tccaggaacg ccgccagccg cagcgagcgg tcctgctccg cgcccggcat
     7081 cggaaacagg aactccgcga ctcccgtgag cttgcggttg ccgccggtgg cgtcgccgtt
     7141 ctcgtccttc gggccgagcg agaaagagcg aaagccgcgc accgacccgg tgccgccggc
     7201 gtagtagttc ttgaagaaag gcagcggctg ctcgccgtag ccgccggccc agccgatttc
     7261 gccgcccagg tgaagcgtaa aggtccgcgt catcgggtag taccactgct gctggtagcc
     7321 gagccggtaa tactgcaggt cgccgcccgc gatctcgccg gaggcgcgca ggaaagagcc
     7381 cgccgtggtg gtgatcaggc tgtcgcgcgt gtcgcgcgtc cagcctgcgg tgcccgaccc
     7441 gtaggtgtac tgggtgccga agtcgcgcac gaatcggatg tacgcaagcg ggctggtggc
     7501 gaagatctcg aggtcgaccg actcgatcgc ggcgccgaaa tcgaccgaga tctgctcgga
     7561 gaccgggtag ccgaacttga tgccgccgcc gagcgagtcg gtcttgtagg cgccggtgtc
     7621 gaggctggag gcgtcggtca cgcgccggta gatgtcgaag ccgcggctca ctccgtccac
     7681 ggtgtagtac gggtccatgt aggacaacga atagacctgg ttcacgctgc cgctgttgat
     7741 gttggccgag atgtactttc cggtgccgaa gacgttcgcc tgcgagaccg acgccgacag
     7801 cgcgatgcgc tccacgctgg agaacccgac gccgaggagc agcgacccgg tcgccttctc
     7861 cttcacggtg tagagcacgt cgacctggtc cgggctcgct tcgaccggca gggtctcgac
     7921 cgtgacctcg ctgaagaagg cggtgcggtc gatgcgccgg cgcgagagct ggatcttcga
     7981 agcgtcgtag tacgcgccct cgagctggcg catctcgcgc cgcaccacct cgtcgcgcgt
     8041 cttggcgttg ccggccacgt cgatgcgccg cacgtacacg cgccggcccg ggtcgatgac
     8101 gatgctgaag gcgacggcgc gcttttcctt gtcgatgttc gggatcgcgt tcgcgttggc
     8161 gaaggcgtag ccctcgttgc ccaggcggtc cgagatcgcc ttggtgctgg cggcgagctt
     8221 ctcgcgcgag aacacgtcgc cgcgcttgag ctggatcagc gtttccagct cctcgcgcgg
     8281 cagcaggagc tgcccggcga ggctcacctc cgacaccgtg taggtttcgc cctcggacat
     8341 gttgatcgtg atgtagatgt cgcgccggtc cggcgtgatc gccacctggg tcgactccag
     8401 ggcgaagtcg aggtagcccc ggttgaggta gaacgagcgc agcgtctcga ggtcggcgcc
     8461 gagccgctcg cgcgagtagc ggtccgcctg cgtgtaccag gtgagccagc cgggcgtgcg
     8521 cagcacgaac aatcccagca gctcgctctc ggtgaatgcc tgcgcgccga cgatgttgat
     8581 gccgcggatc ttggcgaccg ggccctcggt caccgcgatg ctgatgccga cgcggttgcg
     8641 ctcgagcggc gtgaccgtag tctgcaccgc ggcggcgtac atccctttcg acaggtattg
     8701 gcgcttgagc tcctgctcgg cggagtcgag caatgcgcgg tcgaagatgc gcccttccgc
     8761 catgccgagc tcgcgcagca ccttgcgcac gttgtcgggc tcgaactcct tcatgccgga
     8821 gaaatcgatc tgcgcgatcg ccgggcgctc gcgcacgaac accaccagca cgtcgttctc
     8881 gacgtcgagg cgcacgtcgg cgaagaaccc ggtggcatag agagcgcgca gagccgcgcg
     8941 ggccttgtcg gcgttcatcg tctcgccgac cttgaccggg aggtagctga acaccgtgcc
     9001 gggctcggtg cgctgcaggc cctcgacgcg gatgtccttg accacgaacg gctggaattc
     9061 ctgcgcggcg gccgggttcg ggacaagcgc ggccgccacg gcgagaacga gcgcgaagac
     9121 cgcggcgaag acgccggtgt tgcggagcat ctaggaaaga gccgaagcag tcgtccccgc
     9181 gaaggcgggg accccgttta gaaaatcaga aagcctgcgc agaaaggtcc tctccctttg
     9241 attgactggg tccccgcctt cgcggggacg accgccgcat gccttcccta gccggtcagc
     9301 aggcgattca ggtcgttgta gaaggcgaag gccatcatga ccagcagcag cgcgagcccg
     9361 acacgctgcc cgagctccat cgcgcgctcg gagaccggct tgcccttgac gatttcgatg
     9421 gcgtagtaca tgaggtggcc gccgtcgagc agcgggatcg gcagcaggtt cagcacgccg
     9481 aggctgatgc tgatcagcgc gaggaaggtg aggtacgaaa cccagcccat ctgcgccgac
     9541 tggccggcga aatcagcgat cgtcaccggg cccgacaggt gcttccacga cacttcgccg
     9601 accagcatct tgccgagcat cttcaggctg aacaccgcga tgtcgcccgt cttgacgatc
     9661 gacttccaca ggctttcgcc caggccgtgc tgcacccgga tcagcatctt ctcggcgtgc
     9721 gacgccggca cctgcggcgc ggcgccgatg cggccgatgc gtttggggcc ggcggcgacc
     9781 gccgcgggca cgacttccac ggcctggcgc gcgccggcgc gctcgatcgt cagcgcgagc
     9841 ggcatctcgg gccgcgcctg caccgcggcg accagcgcct cccacgactc caccggacgt
     9901 ccgtcggcga gcagcacgcg gtcgccgggc gcgaggccgg ccttctccgc cgcgccgccg
     9961 cgcaccacct ggccgatcac cggctcgagc ggcgggcgat agaggcgcag gccgatgcgc
    10021 tccagggcgt cggactcgac gtcgtccgtc gggaatgagc gcagatcgag ggtcacggca
    10081 ttgagatggc cgcggtcgtt cacgatctcc agccgcagcg gcgagcgctg cagcgccgat
    10141 tgcagcacgc gccagcgcag ttcctgccag gtcgtgatca gctcgccttc gactgagcgc
    10201 acggtgtcgc ccgagcgcag ccccgcggcg gccgcgaccg tgccttcagg cggcgtgccg
    10261 agcaccgggc gcgcttccgg cagcccgtac atgaagaggc ccgcgtacgc cgcgatcgcg
    10321 aacaggaagt tcgccaccgg cccggccacc acgatcccga agcggcgcca gacgctctgg
    10381 cggttgaacg cgcggtgcac ctcgcgcggc tcgaccgggc cctcgcgctc gtcgagcatc
    10441 ttcacgtagc cgccgaacgg gatcgcggcg atcacccact cggtccggtc cgcgccgatc
    10501 tcccgggtca gcaacggacg gccaaagccg accgagaagc gcagcacctt cactccgcac
    10561 aaccgcgcga tcaggtagtg cccgtactcg tgcaccacga tgagcaggcc gagggccacg
    10621 atgaaggcga cgatggtgtg gatgaggctc atgtctcgcc tttcgacccc ctgttaaggg
    10681 ggtcgtgagc cgaaggcgaa cggggggttt ggtttttggt gaaaacaaaa ccccccgctc
    10741 gcgttgctcg ctccactctt atcagggggg caaaaagaat cgcgttcatg cggcaagcgc
    10801 caggacccgc agccgcgccg ccttgcgcgc ctgcgcatcc gcttccatga cttcggcgag
    10861 atcgcccgcc gctcccgccg gcagcgccgc gagcgtgtcg gcgatgacgc cggcgatgcc
    10921 ggtgaacgca agccgtccgg cgaggaacgc ctccacggcg atctcgttcg cggcgttcag
    10981 gatcgcgggc gcggtgccgc cggcgcgcag cgcggcataa gcgagcccga ggcaggggaa
    11041 gcgcaccagg tccggcttct caaaggacag ggtcttgagg gaagccagat cgagcgattg
    11101 cgcgccggac tccaagcgct ccgggtaggc gagcgcgttc gcgatgggca cgcgcatgtc
    11161 cggattcgag agctgcgcga tcaccgagcc gtcggcgtat tcgaccagcg cgtgcaccac
    11221 gctctgcggg tggatcagca cctcgatgcg ctcgggcgcg aggtcgaaca gccagcgcgc
    11281 ctcgatcacc tcgagcccct tgttcatcat ggtcgccgag tcgaccgaga tcttgcgccc
    11341 cattacccaa ttgggatgcg cgcaggcctc cttcggcgtc acctggctca ggctctccgg
    11401 cgcggccgcg cggaaggggc cgcccgaagc ggtgagaata attctgcgag gattccttgc
    11461 actgcccagg cattgaaaaa cggcattgtg ctcgctgtcg accggcagga gctgcgctcc
    11521 cgactcgctc gccgcgtgca tcacgagcgg cccggccatg acgagggctt ccttgttcgc
    11581 gagcagcacg cgcttgccgg cgcgtaccgc ggcgagcgtc gaggcgagcc cggccgcgcc
    11641 gacgatcgcc gccatcaccg tttgcgtttc cggatgccgg gcgacgactt caagcgcttc
    11701 cggaccgaac agcagttcag tgtcgaactc ggagaaacgc cgtttgagcg cgcgatcttc
    11761 ccccactgcg
//
LOCUS       DATBSZ010000184         9185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1192358, whole genome shotgun sequence.
ACCESSION   DATBSZ010000184 DATBSZ010000000
VERSION     DATBSZ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192358"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VIG70_11920"
     CDS             complement(<1..898)
                     /locus_tag="VIG70_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8251305.1"
                     /translation="MTQFARHAIRKHRLDVNSYPDFHHWTVENPELFWTEVWEWCGVI
                     ASRKGNTVLVDGDKMPGARWFPEARLNFAENLLRRGDRGDALVFWDESGAQRRVSYSD
                     LTSEVSRAVQALQALGLRAGDRACAFIPNLPETAMLALAALSQGIVWSSCSPDFGVDG
                     VVERFGQIEPKVLFCADGYRYNGEQHDSLARVAEIVEKLPSVRKVIVVPHLDPNVDVS
                     DVPKAVRLDEWLRRYTPGEIAFAQLPFDHPVYILFTSGTTGKPKCIVHGAGGSLLAGL
                     KMHKLQLDIRPGDRYFYFCTTNW"
     gene            992..3910
                     /locus_tag="VIG70_11925"
     CDS             992..3910
                     /locus_tag="VIG70_11925"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEY8251306.1"
                     /translation="MDSIRRAHRIAEAEAVARLRGLQPDPDTSERISLTALRLARRVR
                     AAPPGALSAESFLRSYGLTTPEGVALMCVAEALLRIPDPETQDALIRDKLASGNWNPA
                     SAADWALMLTGTLARWHSAENENLSTIKKLIARLGEPVVRAAVRQAMRILAGQFVVAE
                     TIEEAVARAAERRPYRFSFDMLGEAARTAEDALSYFNSYSRAIRAVSPPDAVSIKLSA
                     LHPRFEEAKRARVLGELVPRVRSLAKLASERGARITLDAEESERLELTLDIFDALSSE
                     FPIGLAVQAYQKRALPVCEWLAGLRRRITVRLVKGAYWDGEIKRAQQLGMPDYPVFTR
                     KSATDLSYVACARTLLSAPEYIHPAFATHNCRTVATILALGGQFEFQKLFGMGDALYD
                     ALLGERPDVAVRIYAPVGGFTDLLPYLVRRLLENGANTSFVHQIADPHVPVEALVADP
                     VASLPEPYAPDPRIPVPRHLYPDRQNSLGLDLANQKNLNDLKQRTLADRAAEPLSAVE
                     DTSPQELDFSIARAADAFETWSQAPAHERAALLERAADGLEARMVELVSLIVREGGRT
                     YNDAVAEVREAADFCRYYALQARRNFGTPMTLAGPAGERNELRLHGRGVFACISPWNF
                     PLAIFTGQVAAALAAGNTVIAKPAEQTPRIGFRALEILYAAGIPPDAACCVVGPGETI
                     GAALVADPRIGGVAFTGSVETAKRINQALAKRDGPIVPLIAETGGVNAMIVDASALPE
                     QVVDDVVISAFQSAGQRCSAQRVLFVDEGAAPRVLRLLSGALAELKVDDPVEPDTDIG
                     PIIDAPSRNALLFHVQALRTKAKLIGEAKAPATGNYIAPIAFQLPLDELPKVEVFGPI
                     LHVCTYRRDELGQVLQWLRDIGYGLTLGIHSRIQTFVDDVVRGARVGNIYVNRSMIGA
                     VVGVQPFGGEGLSGTGPKAGGPHYMLRFATERTVTVNTAAVGGVTELLAGNEGAQRAS
                     "
     gene            complement(3895..5058)
                     /locus_tag="VIG70_11930"
     CDS             complement(3895..5058)
                     /locus_tag="VIG70_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY8251307.1"
                     /translation="MGAGGEGVRRARRLTSITKSQARGIWLRAQRLDQAAPFGDGLGA
                     TRAAVEHLGYVQIDTIHVVERCHHQILYTRIPGYQRSHLHQAQSIDKTVFEYWTHALS
                     YVPTRDLRYYVRAMKQDWQARSAWFGAVKEADLRKVLARIRKRGALTIRDIDDDVLVE
                     KEHEWASRKPSKRALQLAFYRGLLTVSQRSGMLKTYELTNRHFGWERLPKPASEREIL
                     DYLLDRALRSQGIVSVDSVCYQDARRKPAMRRLIESRVRRGELVAVEFAGEHWLQPEA
                     LEATHEPHNERVHILSPFDPLVHQRKRLQLFFDYEHRFEAYVPKPKRRFGYFAQPVLV
                     GDEIVAVLDLKTDRARQNLLVQQWTWIGKHSRRLRKRQIEEALDRFERFQLAR"
     gene            complement(5016..5981)
                     /locus_tag="VIG70_11935"
     CDS             complement(5016..5981)
                     /locus_tag="VIG70_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8251308.1"
                     /translation="MRIVGRLAAALVISVVTQTLFAQPYPSKPVRIVVNFPAGGVADL
                     YGRIIAAKVQEAWGQPVVVENRTGAGGNIGADAVAKSAPDGYTLNMSAIGPHAINVSL
                     FSKMPYDPVKDFAAVALVLEAEGLLVLHPLIPASSVAELIAYARANPGKLTFASAGMG
                     TASHLAGELFKSMAKVDMTHVPYKGNVPAITDLLAGQTSLLFATMPTVLPHAKAGKLR
                     ALATIGSVRSMAAPELPTIGESLPGFEVNNWIGLFAPAGTPPEIVRRWNGEVMRIMQA
                     PDIQARLPAEGARFSPNTPEQFAAFVKSEIAKWAPVVKASGARVD"
     gene            6030..8945
                     /locus_tag="VIG70_11940"
                     /pseudo
     CDS             6030..8945
                     /locus_tag="VIG70_11940"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
     gene            complement(8937..9185)
                     /locus_tag="VIG70_11945"
                     /pseudo
     CDS             complement(8937..9185)
                     /locus_tag="VIG70_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
BASE COUNT         1451 a         3172 c         3105 g         1457 t
ORIGIN      
        1 cccaattggt ggtgcagaaa tagaaatagc gatcgcccgg ccggatgtcg agctgcagct
       61 tgtgcatctt cagcccggcg agcagcgagc cgccggcgcc gtgcacgatg cacttcggct
      121 tgccggtggt gcccgaggtg aacaggatgt ataccgggtg atcgaacggc agctgcgcga
      181 acgcgatctc gcccggcgta tagcgccgca gccattcgtc caggcgcacc gccttcggca
      241 cgtccgacac gtccacgttc gggtcgaggt gcggcaccac gattaccttg cgcaccgacg
      301 gcagcttctc gacgatctcg gcgacgcgcg cgagcgaatc gtgctgctcg ccgttgtagc
      361 ggtagccgtc ggcgcagaac agcaccttgg gctcgatctg gccgaagcgc tcgaccacgc
      421 cgtcgacgcc gaagtcgggc gagcacgacg accagacgat gccctgcgac agcgcggcga
      481 gcgcgagcat cgcggtctcg ggaaggttgg ggatgaacgc gcaggcgcgg tcgccggcgc
      541 gcaatccgag agcctgcagc gcctgcaccg cgcgcgacac ctcgctcgtc aggtccgagt
      601 aggaaacccg gcgctgcgct cccgactcgt cccagaacac gagggcgtcg ccgcggtccc
      661 ctcggcgcag gaggttctcg gcgaagttga ggcgcgcctc gggaaaccag cgcgcgcccg
      721 gcatcttgtc gccgtcgacc agcaccgtgt tgcccttgcg cgaggcgatg acgccgcacc
      781 attcccagac ctcggtccag aagagctccg ggttctcgac cgtccagtgg tggaagtcgg
      841 ggtagctgtt gacgtcgagg cggtgcttgc ggatcgcgtg gcgcgcgaac tgcgtcacct
      901 gcgcttcttc aatagcgcgt tcggagggct gccaaaggac cggcggggtc gacatatcgg
      961 gcgtaggatt tcgcgcggga attatatgca gatggactcc attcgccgcg cccatcgcat
     1021 cgccgaagcc gaggcggtgg cgcggctgcg gggcctccag cccgatccgg acacttcgga
     1081 gcgaatcagc ctcaccgccc tgcgcctcgc gcggcgggtg cgcgccgccc cgccgggggc
     1141 gctctccgcc gagtccttcc tgcgcagcta cggcctgacc acgcccgaag gcgtggcgct
     1201 gatgtgcgtc gcggaagcgc tgctccgcat cccggatccc gagacgcagg acgcgctgat
     1261 ccgcgacaag ctcgcgtcgg gcaactggaa cccggcgagc gcggccgact gggcgctcat
     1321 gctcaccggc accctcgcgc gctggcacag cgccgagaac gagaacctct cgacgatcaa
     1381 gaagctgatc gcgcgcctcg gcgagcccgt ggtacgggcc gcggtgcgcc aggcgatgcg
     1441 catcctcgcc ggccagttcg tggtcgccga gacgatcgag gaggcagtcg cgcgtgccgc
     1501 cgagcgcagg ccctaccgct tctccttcga catgctcggc gaggccgcgc gcaccgccga
     1561 agatgccttg agttacttca attcctattc aagagccatc cgggcagtca gcccgccgga
     1621 tgccgtctcg atcaagctgt ccgcgttgca tccccgtttc gaagaggcga aacgcgcgcg
     1681 tgtcctcggc gagcttgttc cccgcgtgcg ctcgctggcg aagctggcga gcgagcgcgg
     1741 cgcccgcatt accctcgacg ccgaggaatc cgagcgcctg gagctgacgc tcgatatctt
     1801 cgacgcgctt tcgagcgaat tcccgatcgg gctcgccgtc caggcgtacc agaagcgcgc
     1861 gctccccgtc tgcgaatggc tcgccggcct gcgccggcgc atcacggtgc gactggtgaa
     1921 aggcgcctat tgggacggag agatcaagcg cgcgcagcag ctcggcatgc ccgactatcc
     1981 ggtcttcacc cgcaagagcg cgaccgacct ctcctatgtc gcgtgcgcga ggacgctgct
     2041 ctcggcgccc gagtacatcc atccggcctt cgccacgcac aactgccgaa cggtcgcgac
     2101 gatcctcgcg ctcggcgggc agttcgagtt ccagaagctc ttcggcatgg gcgacgccct
     2161 gtacgacgcg ctcctcggcg agcgccccga tgtcgcggtc cggatctatg cgccggtcgg
     2221 cggttttacc gatctcctgc cttacctcgt gcggcggctg ctcgagaacg gcgccaatac
     2281 ctctttcgtg caccagatcg ccgacccgca cgttccggtc gaggcgctgg tcgccgaccc
     2341 cgtggcctcg ctacccgagc cttacgcgcc cgatccgcgc attcccgtgc cgcgtcacct
     2401 ctatccggat cggcagaact cacttggctt ggatttggcc aatcaaaaaa acctcaatga
     2461 tctgaaacag cgcaccctcg ccgatcgcgc ggccgagccg ttgagcgcgg tcgaggacac
     2521 ttcgccgcag gagctcgact tcagcatcgc gcgcgccgcc gatgctttcg agacctggtc
     2581 gcaggcgccc gcgcacgagc gcgcagcgct cctcgagcgc gccgccgacg ggctcgaagc
     2641 gcgcatggtc gagctcgtgt cgctcatcgt gcgcgaaggc ggccgcacct acaacgacgc
     2701 ggtcgccgaa gtccgcgagg cggcggactt ttgccggtac tacgctctgc aggcaagaag
     2761 gaattttggt acaccgatga ccctcgcggg gcccgccggc gagcgcaacg agctgcgcct
     2821 ccacggccgc ggcgtgttcg cctgcatcag cccctggaac ttcccgctcg ccatcttcac
     2881 cggccaggtc gccgccgcgc tcgccgccgg caacaccgtg atcgccaagc ccgccgagca
     2941 gacgccgcgc atcggctttc gcgcgctgga aatcctctac gccgccggca tcccgcccga
     3001 cgcggcgtgt tgcgtcgtcg ggcccggcga gacgatcggc gccgcgctcg tcgccgaccc
     3061 gcgcataggc ggcgtggcgt tcaccggctc ggtcgagacc gcgaaacgca tcaaccaggc
     3121 gctcgcgaag cgcgacggcc cgatcgtgcc gctcatcgcc gagaccggcg gcgtcaacgc
     3181 catgatcgtc gacgcttccg ccctgccgga gcaggtggtc gacgacgtcg tgatctcggc
     3241 gttccagagc gccggccagc gctgctcggc gcagcgcgtc ctcttcgtcg acgagggcgc
     3301 cgcgccgcgc gtgcttcgcc tcctctcggg cgcgctcgcc gagctgaaag tggacgaccc
     3361 cgtcgagccc gacaccgata tcggtccgat catcgatgcg ccgtcacgga atgccctgct
     3421 cttccatgtt caagcgctaa gaacaaaggc gaaactgatt ggcgaggcga aagcaccggc
     3481 aaccggcaac tacatcgccc cgatcgcctt tcagctcccg ctcgacgaat tgcccaaggt
     3541 cgaggtgttc ggccccatcc tgcacgtctg cacctaccgc cgcgacgaac tcggccaggt
     3601 gctgcaatgg ctgcgcgaca tcggctatgg cctgacactg ggcatccaca gccgcatcca
     3661 gaccttcgtc gatgacgtcg tgcgcggcgc gcgcgtcggc aacatctacg tgaaccggag
     3721 catgatcggc gcggtcgtcg gcgtgcaacc tttcggcggc gaaggcctgt cgggcaccgg
     3781 ccccaaggcc ggcgggccgc actacatgct gagattcgcc accgagagaa cggtcacggt
     3841 caacacggcc gcggtcggcg gagtgaccga gctgctcgcc gggaacgaag gggctcagcg
     3901 cgcgagctga aagcgctcga agcggtccag cgcttcctcg atctgccgct tgcgcaagcg
     3961 ccgcgagtgc ttgccgatcc aggtccattg ctgcaccagc aggttctgcc gtgcgcgatc
     4021 ggtcttgaga tcgagcaccg cgacgatctc gtccccgacg agtacgggct gggcgaagta
     4081 gccgaagcgc cgcttcggct tcgggacgta ggcctcgaag cggtgctcgt agtcgaagaa
     4141 aagctgcagc cgcttgcgct gatgaaccag cggatcgaag ggagacagga tgtggacccg
     4201 ctcgttgtgc ggttcgtgcg tcgcctccag ggcctccggc tgcagccaat gctctcccgc
     4261 gaattcgacc gctaccagct ccccgcgccg taccctcgac tcgatcaatc gccgcatcgc
     4321 gggcttgcgc cgagcgtcct gatagcacac cgaatcgacg ctcacgatgc cttgcgagcg
     4381 cagcgcacgg tcgagcagat agtcgaggat ctctctctcc gacgcgggtt tgggcagccg
     4441 ctcccagccg aagtgccggt tcgtgagctc gtaggtcttg agcatgccgc tgcgctggct
     4501 gacggtcagc aagccgcgat agaaggcgag ctgcagcgcg cgcttcgagg gcttgcggct
     4561 cgcccactcg tgctcctttt ccaccagcac gtcgtcgtcg atgtcgcgga tcgtgagggc
     4621 gccgcgcttt cggatccggg ccagcacttt gcgcagatcc gcctccttca ccgcgccgaa
     4681 ccaggcgctg cgggcctgcc agtcctgctt cattgcgcgg acgtagtagc gcaggtcgcg
     4741 cgtcggcaca taggacagcg catgcgtcca gtattcgaag accgtcttgt cgatgctctg
     4801 cgcctggtgc agatggctgc gctggtatcc cggaatgcgc gtgtacagga tctggtggtg
     4861 gcagcgctcg accacgtgga tcgtgtcgat ctgcacgtaa cccagatgct ccaccgcggc
     4921 gcgcgtcgcc ccgagtccgt cgccgaacgg cgcggcctgg tcaagccgct gggcgcgcag
     4981 ccagattcct ctggcttgag atttggtgat ggacgtcagt cgacgcgcgc gccggacgcc
     5041 ttcaccaccg gcgcccactt cgcgatttcg ctttttacga aggcggcgaa ctgttcgggc
     5101 gtgttcggcg aaaagcgtgc gccctcggcg ggcaggcggg cctggatgtc gggcgcctgc
     5161 atgatgcgca tcacctcgcc gttccagcgc cgcacgatct cgggcggggt accggcgggg
     5221 gcgaagaggc cgatccagtt gttgacctcg aagccgggaa gcgactcgcc gatcgtcggc
     5281 agctcgggcg cagccatcga gcggaccgag ccgatcgtgg cgagcgcgcg cagcttgccc
     5341 gccttggcgt gcggcagcac ggtcggcatg gtggcgaaga ggagcgaggt ctggccggcg
     5401 aggaggtcgg tgatcgccgg cacgttgcct ttgtacggga catgggtcat gtcgaccttg
     5461 gccatcgact tgaagagctc gcccgcgagg tggctcgcgg tgcccatgcc ggcggaggcg
     5521 aaggtgagct tgccgggatt ggcgcgcgcg taggcgatca gctccgccac gctgctggcg
     5581 ggaatcaagg ggtggagcac gagcaggcct tcggcttcca gcaccagcgc gaccgccgcg
     5641 aaatccttga ccgggtcgta cggcatcttt gagaacaggc tcacgttgat ggcgtggggg
     5701 ccgatcgcgc tcatgttgag cgtgtagccg tccggtgcgg acttcgccac ggcgtctgcg
     5761 ccgatgtttc cgccggcgcc ggtgcggttc tccaccacca ccggctgccc ccacgcttcc
     5821 tgcaccttcg ccgcgatgat gcggccatag aggtcggcaa cgccgccggc ggggaagttc
     5881 acgacgatgc gcaccggctt cgagggatag ggctgcgcga agagagtttg tgtgacaacg
     5941 gagatgacca aagcggcggc aaggcgacct acaatgcgca ttctcgattc ctccctcttg
     6001 tgcggctagt ttagccgagg ccaatacgca tggaagccaa gaccagggcg cgcgattccg
     6061 atctcctcga ggttttcggc cgcctgtatt acgcagaggt cgatccggag gagctcgccg
     6121 cgcgcgcggc cgcggacttg cagggcgccg ccgcggcgca cctcgcgttc ggcaaggttt
     6181 tttccggcgg gaaagcgaag gtccgtgcgt acaacccggc gcgcgagcag cacgggtggg
     6241 cgagcacgca taccgcgatc gagatcgtca acgacgacat gccgttcctg gtcgactcgg
     6301 tgaccatgga ggtcaacagc caggggctga agctgcatct cgtgatccat ccggtgctgc
     6361 gcgcccggcg cggccccaag ggggaattgc tttcggttga aaaacccggg gcgtcgccgg
     6421 aagggcggct ggagtcgttc attcatctcg aagtcgaccg ccagaccgac ccggcgaagc
     6481 tcgcccagct cgaggcgggg atcgccaggg tgctcgccga cgtgcgcgct gcggtcgagg
     6541 actggaagcc gatgcagggg cgcctgcgcg aggtcatcgc cgggctcgac gcggcgcgca
     6601 gcggcgtagc gcaggccgag ctcgaggaag ggcgcgcctt tctcgaatgg ctcctcgagg
     6661 acaatttcac cttcctcggc tgccgccgat acgagatcgc caccgccggc ggcgaggacg
     6721 tgctgcgcat cgttccgggc agcggcctcg gcatcctgcg cgagcgcccg ggcgagacgg
     6781 tctcggcgag cttcgccacc ctgcccgccg aagcgcgcaa gcgcgcgagg gtgaaggagc
     6841 tcctgttgct caccaaggcg aacgcgagct ccaccgtgca ccggcccggc cacctcgact
     6901 acgtcggcgt caagcacttc gacgccaaag gggaagtgcg cggcgagacg cgctttctcg
     6961 gcctctacac ccacacggcc tacgcgtcga atccgaccga ggtccccctg ctgcgccgca
     7021 agctcgccgc ggtgatggag cgcgccagcc tgctgcccgg gggccacagc ggcaaggcgc
     7081 tcgcctcgat cctcgagcgc tacccgcgcg acgagctgct gcagatcggc gccgacgacc
     7141 tgcagcgcca cgccatggcg atcctgcagc tcggcgagcg ccagcggttg cgcctgctgg
     7201 tgcgccacga cgcgtacggg cgcttcgtga cctgcctcat cttcgtgccg cgcgagcgct
     7261 tcaataccga gctgcgccag cgcttccaga agatcctgac cgaagcgttc aacggcgtgt
     7321 cctcggactt cgacgtggat ctgtcgtcgg cggcgctcgg ccggatcctc atgcgcatcc
     7381 gcaccgggcc cggtgcgatg gtgggaacgc ccgacctcaa ggcgctcgag gggcgcctgg
     7441 tcgaagcgat gcgccgctgg gaggacgacc tcctcgcggc gctggtcgag cgccacggcg
     7501 aggagcgcgc gaacgccttg ttccgcatct atgaaaaagc attccccggc agctaccgcg
     7561 aggaatactc ggtgcgcgag gcggtcaccg acatcgagat gatcgagtcg ctcgacccgg
     7621 ccgtcgacct cggcatgaac ctgcaccggc gcgcggacgg ccggccggga atgctcaact
     7681 tcaagatcta ccgccgcggc agcaccgtgc cgctctccga cagcgtgccg atgctcgagc
     7741 gcctcggcgt gcgcgtgcag tacgagcatc cgcacgagct cgacccggcg ggcgcgcagc
     7801 cggtgcgcgt ggtcgacttc gggctggtgc cggccgccgg cgccgcgctc gagctcgccc
     7861 aggtgcgcga cgctttccac gacgcgttcc tcggcatgtt ctgcggcacg atcgaggcgg
     7921 acagcctcaa tcgcctcgtg ctcacggcgg ggctgtcggc gcgagagatc agcgtgctgc
     7981 gcgcctacgc gcggtatctg cgtcagggca cctcgacctt cagcctcgcc tatgtcgagc
     8041 aggcgctcgc cgcccacccg cgcatcgccg cgcttctggt gcgcctgttc ttcgcgagat
     8101 tcgagggccc cgggaaggag cgcgaggcga ccctggcagg ggaaatttcc gctgcgctgg
     8161 acgaagtgca gaacctggac gaggaccgca tcctgcgcag cttcctcgcg gtgattcaag
     8221 ccaccacgcg cacgaactac tttctgggga agccgtacgt ctcgttcaag ctcgacccga
     8281 agcgcgtgcc cgggctgccc gagccgcgac cgatgttcga gatctgggtg tactcgccgc
     8341 gcgtcgaggg cgtgcacctg cgcggcgggc gcgtggcgcg cggcggcctg cgctggtcgg
     8401 accgcatgga ggacttccgc accgaggtgc tgggcctcat gaaggcgcag atggtgaaga
     8461 acgtggtgat cgtcccggtc ggcgccaagg gcggcttcgt ggtgaagcgg ccgcccgcgg
     8521 aacgcgaggc gttcctgaag gaaggcgtcg cctgctacca gacgttcctg cgcgggctgc
     8581 tcgacatcac cgacaacctg gccggcggga aagtggtgcc gccgaagggc gtggtgcgcc
     8641 acgacaacga cgatccctac ctcgtagtcg ccgccgacaa gggcactgcg accttctccg
     8701 actacgccaa cgccatcgcc agggagtacg gcttctggct cgacgacgcg ttcgcctcgg
     8761 gcggctcggc gggctacgac cacaaaaaga tggcgatcac cgcgcgcggc gcctgggaat
     8821 cggtgaagcg ccacttccgg gagatgggcg tcgacaccca gagccaggac ttcatcgtcg
     8881 cgggcatcgg cgacatgtcg ggcgacgtgt tcggcaacgg catgctgctg tcgcggcaca
     8941 tccgcctcgt cgcggccttc gaccaccgcc acatcatcat cgaccccgac cccgacacgc
     9001 aggccgtcgt tggcacgatc gccgacctcg gcgtgcgttt ccgcgctcgc cttcacgtac
     9061 gtgccgatgc cgccgttgta gagcaggtcg accggggcgc gcaggatggc gcggatgagg
     9121 tcggcgggcg tgcacgcggc gagcgcggga tcgatgccga gtgcggtgcg cacgtcgggt
     9181 ccgat
//
LOCUS       DATBSZ010000185         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_235866, whole genome shotgun sequence.
ACCESSION   DATBSZ010000185 DATBSZ010000000
VERSION     DATBSZ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235866"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VIG70_11950"
     CDS             <1..893
                     /locus_tag="VIG70_11950"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY8251309.1"
                     /translation="EEDLRGYRAVERAPVCGPYRRWRLCSMGPPSSGGIAVLQILGLL
                     ERTSFARAPPESAAALHFFAEAGKLAYADRARYLGDPDFVSVPVKALLGSNYLEKRAK
                     LIGERAVPLAPPGDSEAGTSHFSIVDARGNIVSMTTTIESAFGSRLMVRGFLLNNQLT
                     DFDFAPGGPNSVAAGKRPRSSMAPAVVFNSNDQPYLVIGSPGGSMIINYVAKALVGVL
                     DWGLDVQAAIDLPNFGSRNGPTLIEQGSRYGALAPDLLQRGHQVETIVLTSGLHGIER
                     VPGGWRGGADPRREGAVRGE"
     gene            895..>3359
                     /locus_tag="VIG70_11955"
     CDS             895..>3359
                     /locus_tag="VIG70_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251310.1"
                     /translation="MKARKVSLGKTTRPSAAGVLPRKRLFELLKKESPVTWVTGPPGC
                     GKTTLAASWLDHAGVRSVWYQLDEGDADVATFFYYLSLAAASLDGERERLPLFTPEYQ
                     PGLGVFTRRYFERLYAQLKAPFALVFDGYQSVPASSQLHEVMRVALETLPPGGRVLTI
                     SRGDPPATLTRLRANQALSVIGWDELRLTREEAGSIIARRRPGFSAGAVDALYARTQG
                     WAAGLVLMLEQAKVSGAIAEPPGLASRQLVFDYLAGEIFQKSDARTQQFLLHTAYLPE
                     MTTAMARELSADEGTGELLAGLNRNNYFVSVRDTQPEPVYQYHPMLRDFLQARAADSL
                     PKERRRELQRASAAQMEQAGRIEDAVALYRECHDWSEMARLIEAHAAALLDQGRGETI
                     ARWVEELPGEVQAKRPWTLYWAASSQAQVAPREGRILYEKTFDLFRAQCDSAGMVLAA
                     SGAMFAILYELDDCSLLDRWIAVLDEAEKDGPLPLRPEAEARVACGMLFSLTLRQPQR
                     RDIKRWIERALAASAGQPDVNLRMFVGLLASLTLMWTGLYGRAAELIAAMRELARASG
                     VTPFSLITLKNIETMYAMLTADGAGCENAMREGLEIAHATGVHTWTFQLLVYGYGGAL
                     GSGNLIQAQALARELATRTANAGRFNLCLYNHFQAWEAILRKDLMTALQREKAALRMA
                     IEVGCPLFEVLCRLALAEILAECNDERKCVAHLHTLRDIVRGIDNRHLEFTCLVGFAQ
                     IALTHGRQRTGMAALRRGLELGREYGYAHFLWWRPAAVARVLANALEAGIEPDYVRSL
                     IKRRALVPEQPALGVEGWPWAYRV"
BASE COUNT          515 a         1176 c         1155 g          513 t
ORIGIN      
        1 ccgaagagga tcttcgcggc taccgcgccg tcgagcgcgc gccggtatgc ggcccgtacc
       61 gccgctggcg cctttgctcg atgggcccgc cgagctccgg cggcatcgcg gtgctgcaga
      121 tcctcgggct cctcgagcgc acgagcttcg cgcgggcgcc gcccgagtcg gcggccgcgc
      181 tgcatttttt tgccgaagcc ggcaagctcg cgtacgcgga ccgcgcccgc tacctgggcg
      241 atccagattt cgtttccgtt cccgtcaagg cgttgctggg ttcgaactac ctggaaaaaa
      301 gagcaaagct gatcggcgaa cgcgccgtgc cgcttgcgcc gcccggcgac agcgaagccg
      361 gcaccagcca tttctccatc gtcgatgcgc gcggcaacat cgtctcgatg accaccacga
      421 tcgagagcgc gttcggcagc cgcctcatgg tgcgcggctt tctcctcaac aaccagctca
      481 ccgacttcga ctttgcgccc ggcgggccga attccgtcgc agcgggcaag cgcccgcgga
      541 gcagcatggc gccggccgta gttttcaaca gcaatgacca accctatttg gtgatcggct
      601 cccctggcgg gtcgatgatc atcaactacg ttgccaaggc gctggtcggg gtgctcgact
      661 ggggtctcga cgtccaggcc gccatcgatc tgcccaattt cggctcgcgc aacgggccga
      721 ccctgatcga acagggcagc cggtacggcg cgttggcgcc agacctcttg caacggggtc
      781 atcaagtcga aacaatcgtg ctcacgagcg gcctgcacgg catcgagcga gtgcccggcg
      841 gctggcgcgg gggcgccgac ccgagacggg agggggcggt gaggggcgaa tgaaatgaag
      901 gcgaggaaag tgtcgctggg caagacgacg cggccgagcg cggccggggt cttgccgcgc
      961 aaacgccttt tcgagttgct caagaaagaa agtccggtga cctgggtcac cggtccgccc
     1021 ggctgcggca agaccacgct tgccgcgagc tggctcgacc acgccggcgt gcgctccgtc
     1081 tggtatcagc tcgacgaagg cgacgccgac gtcgccacgt tcttctacta cctcagcctc
     1141 gccgcagcgt cgctcgacgg cgagcgcgag cggcttccgt tgttcacgcc ggagtaccag
     1201 cccggcctcg gcgtgttcac gcggcgctat ttcgagcgcc tctacgcgca gctcaaggcg
     1261 ccgtttgcgc tggtgttcga cggctaccag tcggtgccgg cgtcgtcgca gctgcacgag
     1321 gtgatgcgcg tggcgctcga gacgctgccg cccggcgggc gcgtgctcac cattagccgc
     1381 ggcgacccgc ccgcgacgct gacgcggctg cgcgccaacc aggcgctctc cgtgatcggc
     1441 tgggacgagc tgcgcctgac gcgcgaggag gccggctcga tcatcgcgag gcgccgcccg
     1501 gggttcagcg ccggggcggt cgacgcgctc tacgcgcgca cccagggctg ggcggcgggg
     1561 ctcgtgctga tgctcgagca ggcgaaggtt tccggcgcga tcgccgagcc gccgggcctg
     1621 gcgagccggc agctcgtgtt cgactacctc gcaggcgaga tcttccaaaa atccgacgcg
     1681 cgcacgcagc agttcctgct gcacaccgcg taccttcccg agatgaccac cgccatggcg
     1741 cgcgagttga gcgccgatga gggaacgggc gagctgctgg ccgggctcaa ccgcaacaac
     1801 tatttcgtca gcgtacgcga tacgcagccc gagccggtct accagtacca cccgatgctg
     1861 cgcgacttcc tccaggcgcg cgccgccgac agcttgccga aggaacgccg gcgcgagctt
     1921 caacgcgcat cggccgctca gatggagcaa gcgggccgca tcgaagatgc ggtcgcgctt
     1981 taccgcgaat gccacgattg gagcgagatg gcgcgcctca tcgaggcgca cgccgcggcg
     2041 ctgctcgacc agggtcgcgg cgagaccatc gcgcgctggg tcgaggagct gccgggcgaa
     2101 gtgcaggcca agcggccgtg gaccctctac tgggcggcgt caagccaggc gcaggtggcg
     2161 ccgcgcgagg ggcgcatcct ctacgagaag acgttcgacc tgttccgcgc gcagtgcgac
     2221 agcgccggca tggtgctcgc tgcttcgggc gcgatgttcg ccatcctcta cgagctcgac
     2281 gactgctcgc tgctcgaccg ctggatcgcg gtgctcgacg aggccgagaa ggacggcccg
     2341 ctgccgctgc ggcccgaggc ggaggcgcgc gtcgcgtgcg gcatgctgtt ctcgctcacg
     2401 ctgcggcagc cgcagcgccg cgacatcaag cgctggatcg agcgcgcgct cgccgcctcg
     2461 gcggggcagc ccgacgtcaa cctgcgcatg ttcgtaggcc tgctcgcgtc gctcacgctg
     2521 atgtggaccg gtctgtacgg ccgcgccgcc gagctgatcg cggcgatgcg cgagctcgcg
     2581 cgcgcgagcg gcgtaacgcc gttctcgctg atcaccctga agaacatcga gacgatgtac
     2641 gccatgctga ctgccgacgg cgccgggtgc gagaacgcga tgcgcgaagg cctcgagatc
     2701 gcgcacgcca ccggcgtgca cacctggacc ttccagctcc tggtctacgg ctatggcggc
     2761 gcgctcggct cgggcaatct catccaggcc caggccctcg cgcgcgagct cgccacgcgc
     2821 accgccaatg ccgggcgctt caacctgtgc ctgtacaacc acttccaggc ctgggaagcg
     2881 atactgcgca aagacctgat gacggcgctg cagagggaga aggccgcgct gcgcatggcg
     2941 atcgaggtcg gctgcccgct gttcgaggtg ctgtgccgcc tcgcgctcgc cgagatcctt
     3001 gcggagtgca acgacgagcg caaatgcgtc gcgcaccttc acaccctgcg cgacatcgtg
     3061 cgcggcatcg acaaccgcca cctggaattc acctgcctgg tcgggttcgc gcagatcgcg
     3121 ctcacgcacg ggcgccagcg caccggcatg gccgccctgc gccgcggcct ggagctcggc
     3181 cgcgagtacg gctacgcgca tttcctctgg tggcggccgg cggcggtggc gcgcgtgctc
     3241 gcgaacgcgc tcgaggctgg tatcgagccc gactacgtgc gcagcctcat caagcgccgc
     3301 gccctggttc cggaacagcc ggcgctcggc gtcgaaggct ggccttgggc ctaccgcgt
//
LOCUS       DATBSZ010000186         6452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_30648, whole genome shotgun sequence.
ACCESSION   DATBSZ010000186 DATBSZ010000000
VERSION     DATBSZ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30648"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3191)
                     /gene="smc"
                     /locus_tag="VIG70_11960"
     CDS             complement(<1..3191)
                     /gene="smc"
                     /locus_tag="VIG70_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY8251311.1"
                     /translation="MRLTQIKLSGFKSFVDPTSIHIPGALVGVVGPNGCGKSNVIDAV
                     RWVLGESRASALRGDSMQDVIFNGSVNRKPIARASVELIFDNSQGRAAGQWSQYAEIS
                     VKRTVQRDGESSYFVNGTHVRRRDITDMFLGTGLGPRAYAIIEQGMISRVIEAKPEEL
                     RVFLEEAAGISRYKERRRETENRLADTRENLARVTDIRLELGAQLEKLEAQAKVATRY
                     KEYHSDLQFKQHLLWYLRRRDAAAERDRHRQELSRITNELESQNAELRSVESRVERAR
                     AAHYGAGDGLNASQGALYAANAEVARFESELRHAEETRSRLDSQHTERRAQLAAWRAQ
                     RADLTQSLHSWAVRAGEAKQRVAETRERSEREGHSLPQVEQAFRTAQEALEAARSALS
                     QAENRLQLEQANGAHLERGQQALEQRRERLEAELQTLGEPQAGSVQNLDDLQQGLATA
                     EQAFARLQAEHAAAEGRRAEAADGLGAAQRDLAAAQAQLETLRQIQADTEDNAPLNEW
                     LGRHGLAHAARLWQKLRIDPGWETAVESVLRERLHALELTDASGLAAALADRPPVKAS
                     IFDHGARPTSAPAAGLPLLTKIHATDPAVAGALADWLAGVVAVEGTPDARMREALPPG
                     VVMVNREGHQFTRHTVSFHAPDAQDAGLLERQAQIEELEKRCRALAAGLSNAEAWQRR
                     HEEEVNSHLASLEQARVGISERQKGLHDAQIENVKVSQAQEHSRERSAQVRSELDEVR
                     GEAERGRQALSDCEGTAARLSREMVEMKERAERAREALAGADSALAAQRSALQQAERE
                     AQDALFGERECSSKIAEIDNSVRVIDQQIARADAEVARLNEELAADPIPAVRESLASA
                     VEARISCEKNLAESRNAVEAAAGALRELEEARLQAEGRAAPLREKVNELRLKEQAAQI
                     NYDQFATQLAEAGADEALLAAEAEGAPRASSLQGEITRITQAINELGAVNLAALEELR
                     SSQERKSFLDSQSGDLEEAVTTLEDAIRRIDRESRELLRETFDTVNGHLAKLFPTLFG
                     GGDAKLTMTGEEILDAGVQVMAHPPGKRNTSIHL"
     gene            3238..3612
                     /gene="queF"
                     /locus_tag="VIG70_11965"
     CDS             3238..3612
                     /gene="queF"
                     /locus_tag="VIG70_11965"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEY8251312.1"
                     /translation="MPSRPSKRLETFANPTPQRDYRIHMEIPEFTCLCPKTGQPDFAT
                     LLLDYVPDRLCVELKSLKLYIWSYRDEGAFHEAVTNRILDDLVRAMKPRYARLEARFN
                     VRGGIHTTVVAEHRKRGWAGKA"
     gene            3609..4745
                     /gene="dapC"
                     /locus_tag="VIG70_11970"
     CDS             3609..4745
                     /gene="dapC"
                     /locus_tag="VIG70_11970"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEY8251313.1"
                     /translation="MNPLLEKLKPYPFEKLRALVGQMSGQMSGDRPAEINLSIGEPKH
                     ATPRLVKDALANALDGLAVYPLTAGTPELREAISGWLARRYGIPAPDPATQVLPVNGS
                     REALFAFAQTLIDPTRNARVVCPNPFYQIYEGAALLAGATPVYGPPEKWDGVQLVYAC
                     SPANPSGQVMRLEDWRRLFELSDRHGFAIASDECYSEIYFDRAPLGALEAAFQLKRKD
                     YRRLVAFSSLSKRSNAPGMRSGFVAGDAGLLEKFRLYRTYHGSAMSLAVQKASIAAWN
                     DEEHVRENRRLYAEKFDAVLPVLNAERPGGGFYVWLRTPIDDTEFVRRLHHEYNVLVL
                     PGSFLGREVNGTNPGANHVRIALVAPLKDCVAAAERIGQLLRKL"
     gene            4742..5533
                     /gene="dapD"
                     /locus_tag="VIG70_11975"
     CDS             4742..5533
                     /gene="dapD"
                     /locus_tag="VIG70_11975"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY8251314.1"
                     /translation="MNAKEVIEAAWEERAALKPTKGLREAVKQAIAGLDSGKLRVAEK
                     SGGQWITHQWLKKAVLLSFRLQDNRVMEKRYYDKVPSKFESFDFGKAGFRVVPPAAVR
                     RGAYIAKNVVLMPSFVNIGAYVDEGTMVDTWATVGSCAQIGRNVHLSGGVGIGGVLEP
                     VQANPVIIEDNCFIGARSEVVEGVIVEENSVLSMGVYLGQSTRILERASGRILYGRVP
                     AGSVVVSGTLPSADGKYSLYCAVIVKTVDAKTRAKTSLNELLRGD"
     gene            5565..>6452
                     /locus_tag="VIG70_11980"
     CDS             5565..>6452
                     /locus_tag="VIG70_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY8251315.1"
                     /translation="MSAMKKLFQIMADKKASDIFVSIGAPINIKINGVAIPVNQATMT
                     ADTVQQLLYEVLNERQIKEFEEEMELNTAYNLAGVGTFRISAFRQKGSPSVVVRYIPG
                     TIPSIDSLGVPEVLKEVIMGKRGLILMVGATGSGKSTSLASMLDFRNERKSGHILTLE
                     DPVEYVFQNKKSIVNQREIGTDTKAFEIALKNALRQAPDCILIGEIRDKPTMSMALAY
                     AQSGHLCLATLHASNSYHAMNRIINFYPLENRPALLLDLSASLSAVVSQRLVRTKTGA
                     RVAAVEVLLNTRHISELIER"
BASE COUNT          981 a         2209 c         2171 g         1091 t
ORIGIN      
        1 aggtggatgc tggtgttgcg cttgcccggg ggatgcgcca tcacctgcac gccggcgtcg
       61 aggatctcct ccccggtcat cgtcagcttg gcgtcgccgc cgccgaagag ggtcgggaac
      121 agcttggcga gatggccgtt cacggtgtcg aaggtctcgc gcaggagctc gcggctctcg
      181 cggtcgatcc tgcggatcgc gtcctcgagc gtggtcacgg cttcctcgag gtcgcccgac
      241 tgcgaatcga ggaacgactt gcgctcctgg ctcgagcgca gctcttccag cgcggcgagg
      301 ttaacggcgc cgagctcgtt gatcgcctgg gtgatgcggg taatctctcc ctggagcgac
      361 gaggcgcgtg gcgcgccctc ggcctccgcc gcgagcagcg cctcgtcggc gcccgcctcg
      421 gcgagctggg tcgcgaactg gtcgtaattg atctgcgcgg cctgctcctt gaggcgcagc
      481 tcgttcactt tctcgcgtag cggcgcggcg cggccttcgg cctggagcct cgcctcctcg
      541 agctcgcgca gcgcgcccgc ggcggcttcc accgcgttgc gcgattccgc gaggtttttt
      601 tcacaggaga tcctcgcctc aactgcagaa gccaaagact cccgtaccgc cggaatcggg
      661 tcggcggcga gctcctcgtt gaggcgcgcc acttcggcat cggcgcgcgc gatctgctga
      721 tcgatcacgc gcaccgagtt gtcgatctcg gcgatcttgg aggagcattc gcgctcgccg
      781 aagagcgcat cctgcgcctc gcgctcggcc tgctgcaggg cgctgcgctg cgcggcgagc
      841 gcgctgtccg cgccggcgag cgcctcgcgc gcccgctcgg cccgctcctt catctccacc
      901 atctctctcg agagccgcgc ggccgtgcct tcgcaatcgg agagcgcctg cctgccgcgc
      961 tcggcctcgc cgcgaacctc gtcgagctcg gagcgcacct gggcgctgcg ctcgcgagag
     1021 tgctcctggg cctgcgaaac cttcacgttc tcgatctgcg cgtcgtgcag ccctttctgg
     1081 cgctccgaga tcccgaccct cgcctgctcg agggaagcaa gatgggaatt gacttcttcc
     1141 tcgtggcggc gctgccaggc ttccgcgttc gacaagcccg cagcgagcgc gcggcagcgc
     1201 ttctcgagct cctcgatctg cgcctggcgc tcgagcaggc cggcgtcctg ggcgtcgggc
     1261 gcatggaagc tcaccgtgtg gcgggtgaac tggtggcctt cgcggttcac catcacgacg
     1321 cccggcggca gcgcttcgcg catccgcgcg tcgggcgtgc cttccacggc caccacgccg
     1381 gccagccagt cggcgagcgc gccggcgact gccgggtccg tggcgtgaat cttggtgagg
     1441 agcggtagcc cggcggccgg cgccgaggtg ggtcgcgccc catgatcgaa gatgctcgcc
     1501 ttgaccggcg ggcgatcggc aagcgcggcc gccagcccgg aggcgtcggt gagctccagc
     1561 gcatgcaggc gctcgcgcag cacggactcg actgcagtct cccagccggg gtcgatgcgc
     1621 agcttttgcc acagccgggc ggcgtgcgcc aggccgtgac gcccgagcca ctcgttcagc
     1681 ggtgcgttgt cctcggtgtc ggcctggatc tggcgcaggg tctcgagctg cgcctgcgcc
     1741 gcggccaggt cgcgttgcgc agcgccgagc ccgtcggcgg cttcggcgcg ccgcccttcc
     1801 gccgcggcgt gctccgcctg caatcgcgcg aatgcctgct cggccgtggc gagcccctgc
     1861 tgcaggtcat cgagattctg aacgctgccc gcctgcggct ccccgagggt ctgcagctcg
     1921 gcttcgagcc gctcgcggcg ctgctcgagc gcctgctggc cgcgctcgag gtgcgcgccg
     1981 ttcgcctgct cgagctgaag gcggttctcg gcctgcgata gcgcgctgcg cgcggcctcc
     2041 agcgcctcct gggcggtgcg aaaggcctgc tcgacctggg gcagcgaatg gccttcccgc
     2101 tccgatcgct cccgggtctc ggcgacgcgc tgcttcgctt ccccggcgcg gaccgcccag
     2161 ctgtgcagcg attgggtcag gtcggcgcgc tgcgcccgcc atgcagcgag ctgcgcgcgg
     2221 cgctcggtgt gctggctgtc gaggcggctg cgcgtttctt cggcgtgcct gagctcggac
     2281 tcgaagcgcg ccacctcggc gttggccgcg tagagcgcgc cctgcgaggc gttcaggccg
     2341 tcgcccgcgc cgtaatgcgc ggcgcgcgcg cgctccacgc gcgactcgac gctgcgcagc
     2401 tccgcgttct gcgattccag ctcgttcgtg atccgggaga gctcctgccg gtggcggtcg
     2461 cgctcggccg ccgcgtcgcg ccgccgcagg taccagagaa gatgctgctt gaactgcagg
     2521 tcggagtggt attccttgta gcgcgtcgcg accttcgcct gcgcctcgag cttctcgagc
     2581 tgcgcgccga gctcgaggcg gatgtcggtg acgcgcgcca ggttctcgcg cgtgtcggcg
     2641 agccggttct cggtctcgcg ccggcgctcc ttgtagcgcg agatccccgc ggcctcctcg
     2701 aggaacacgc gcagctcctc gggcttcgcc tcgatcacgc gcgagatcat gccctgctcg
     2761 atgatggcgt aggcgcgcgg gccgaggccg gtgccgagga acatgtcggt gatgtcgcgc
     2821 cggcgcacgt gcgtgccgtt cacgaagtag ctcgactcgc cgtcgcgctg caccgtgcgc
     2881 ttcaccgaga tctcggcgta ttgcgaccac tgcccggcgg cgcggccctg cgagttgtcg
     2941 aagatgagct cgaccgaggc gcgcgcgatc ggcttgcggt tcaccgagcc gttgaagatc
     3001 acgtcctgca tcgagtcgcc gcgcaaggcg gatgcccgcg attcgccgag cacccagcgc
     3061 accgcgtcga tcacgttcga tttgccgcag ccgttgggcc cgaccacgcc gaccagcgcg
     3121 ccggggatgt ggatcgaggt tggatcgacg aaggacttga atccggacag ttttatttga
     3181 gtgaggcgca cggaaaaaat gataacatcg ccgcgctttc tttccggcgg tttttccgtg
     3241 ccttcgcgac ccagcaagcg gctagaaact ttcgccaatc cgacgccgca gcgcgactac
     3301 cggatccaca tggagattcc ggagttcacc tgcctctgcc cgaagacggg ccagccggac
     3361 ttcgccacgc tgctgctcga ctacgttccc gaccgcctgt gcgtggagct caagtccctc
     3421 aagctctaca tctggtccta ccgcgatgaa ggcgcgttcc acgaagccgt gaccaaccgc
     3481 atcctggatg acctggtgcg ggcaatgaag ccgcgctacg caagactcga ggcgcgcttt
     3541 aacgtgcgcg gcgggatcca caccacagtg gtggcggagc atcgcaagcg cggttgggct
     3601 ggcaaggctt gaacccgctc ctcgagaagc tcaagccgta cccgttcgag aagctccgcg
     3661 ccctcgtcgg ccaaatgagc ggccaaatga gcggcgaccg gccggccgag atcaatctct
     3721 cgatcggcga gccgaagcac gccacgccgc gcctggtgaa ggacgccctc gccaatgcgc
     3781 tcgacggtct cgccgtctat ccgctgacgg ccgggacgcc ggagctgcgc gaggcgattt
     3841 cgggctggct cgcgcggcgc tacggcatcc cggcgcccga cccggcgacc caggtgctgc
     3901 cggtcaacgg ctcgcgggag gcgctctttg cttttgccca gacgctcatc gacccgaccc
     3961 gcaatgcgag ggtcgtctgc cccaatccct tctaccaaat atatgagggc gcggcgctcc
     4021 tcgccggagc gacgccggtc tacggccccc cggagaagtg ggacggcgtg cagctggtct
     4081 atgcctgctc gccggccaac ccgagcggcc aggtgatgcg actcgaggac tggcggcgcc
     4141 tgttcgagct ttccgaccgc cacggcttcg ccatcgcgtc ggacgagtgc tactcggaga
     4201 tctatttcga ccgggccccg ctcggcgccc tggaagccgc gtttcaattg aagagaaagg
     4261 attatcggcg gctggtcgca ttctccagcc tgtccaagcg ctcgaacgct cccggcatgc
     4321 gctcgggctt cgtcgccggc gacgccgggc tgctcgagaa gttccggctc taccgcacct
     4381 accacggctc ggccatgagc ctcgcggtgc agaaggcgtc gatcgccgcg tggaacgacg
     4441 aggagcacgt gcgcgagaac cggcgcctgt atgcggagaa gttcgacgcg gtcctgcctg
     4501 tgctgaacgc ggagcggccc ggcggcgggt tctacgtctg gctgcgcacg ccgatcgacg
     4561 acaccgagtt cgtgcgccgc ctgcaccatg aatacaatgt gctcgtgctg cccggaagct
     4621 ttctcggacg cgaggtcaac gggacgaatc ccggggccaa ccatgtccgc atcgcgctgg
     4681 tcgcgccgct gaaggactgc gtcgccgccg ccgagcgcat cggccaactc ctgcgcaaac
     4741 tatgaacgcc aaggaagtca tcgaagccgc ctgggaagaa cgcgccgcgc tgaagccaac
     4801 caaggggctg cgcgaggcgg tgaaacaggc gatcgccggc ctcgattcgg gaaagctgcg
     4861 ggtcgccgag aaatccggcg gccagtggat tacgcaccag tggctgaaga aggccgtgct
     4921 gctttccttc cggctgcagg acaaccgggt catggagaag cgctactacg acaaggtgcc
     4981 gtcgaaattc gagtccttcg atttcggcaa ggcgggattc agggtcgtgc cgccggccgc
     5041 ggtccggcgc ggcgcgtaca tcgcaaagaa cgtggtgctg atgccctctt tcgtgaatat
     5101 cggggcgtac gtcgacgagg gcaccatggt cgacacctgg gccaccgtgg gctcgtgcgc
     5161 ccagatcggc aggaacgtgc atctttccgg cggcgtcggc atcggcgggg tgctcgagcc
     5221 ggtgcaggcg aacccggtga tcatcgagga caactgcttc atcggcgcgc gctccgaggt
     5281 ggtcgagggc gtgatcgtcg aggaaaacag cgtgctgtcg atgggggtgt acctcggcca
     5341 gagcacccgg atcctcgagc gcgccagcgg ccgcatcctc tacggccgcg tgccggccgg
     5401 gtcggtggtg gtttcgggta ctttgccctc tgcggacggc aagtacagcc tgtactgtgc
     5461 ggtgattgta aaaactgttg atgccaagac ccgcgccaag accagcctga acgaacttct
     5521 caggggggat taaactagcg gcaaggcacc aggggagaag cacgatgtct gcaatgaaaa
     5581 agctgttcca gatcatggcc gacaagaagg cctcggacat cttcgtctcg atcggcgcgc
     5641 cgatcaacat caagatcaac ggcgtcgcga tcccggtcaa ccaggccacg atgaccgccg
     5701 acaccgtgca gcagcttctc tacgaagtgc tgaacgagcg ccagatcaag gaattcgagg
     5761 aagagatgga gctcaacacc gcgtacaacc tcgcgggcgt gggcaccttc cgcatcagcg
     5821 cgttccgcca gaagggctcg ccctcggtcg tggtgcgcta catcccgggc acgatcccct
     5881 cgatcgactc gctcggcgtg cccgaggtgc tgaaggaagt catcatgggc aagcgcggcc
     5941 tgatcctgat ggtcggcgcc accggctcgg gcaagtcgac ctcgctcgcg tcgatgctcg
     6001 acttccgcaa cgagcgcaag tcgggccaca tcctgacgct cgaagacccg gtcgagtacg
     6061 tgttccagaa caagaagtcg atcgtgaacc agcgcgagat cggcaccgac accaaggcgt
     6121 tcgagatcgc gctcaagaac gcgctgcgcc aggcgccgga ctgcatcctg atcggcgaga
     6181 tccgcgacaa gccgaccatg tcgatggccc tcgcctacgc gcagtctggc cacctgtgcc
     6241 tcgcgacgct gcacgcgagc aacagctatc acgcgatgaa ccggatcatc aacttctacc
     6301 cgctggagaa tcggccggcg ctgctgctcg acctgtcggc ttcgttgtcg gcggtagtct
     6361 cgcagcgcct ggtgcgcacc aagaccggcg cccgcgtggc cgcggtggag gtcctgctga
     6421 atacccgcca catctcggag ctgatcgaga ga
//
LOCUS       DATBSZ010000187         4908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_509218, whole genome shotgun sequence.
ACCESSION   DATBSZ010000187 DATBSZ010000000
VERSION     DATBSZ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509218"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VIG70_11985"
     CDS             <1..1281
                     /locus_tag="VIG70_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8251316.1"
                     /translation="TEQALARIADPAGEGKRAFIKVYAEGALAEADFSDRLRKAGVRR
                     SPVDGLPVSLKDLFDVAGDLTRAGSRIMEKNPPANADALAVARLRAAGAVFVGRTNMV
                     EFAFGTTGINPHYGTPRNPWDRKTGRIPGGSSSGAAVAQADGMCVMALGSDTRGSIRI
                     PAAFCGVTGFKPTTRRVPREGAFPLSTTLDSVGPLASSVACCAVYDAILAGEPPEPLS
                     ELPAKGLRLLVPKTEVRDGLDPEVASAFSAALEKLGRAGASIAEKPVPAFDRQGEYFK
                     GGGFAGAEAYAIHRKNAERLAEYDPRVAKRVILGKDLSAADYVDFVRLRAEFQREVEA
                     LVAPFDAFLLPTNPCIAPPIAEVDATDEAYFRWNMRILRIVGLVNFLDGCAASLPCHA
                     SGAAPVGLMVCGPAMSDRRVLAVAAAVEQALARN"
     gene            1256..2062
                     /locus_tag="VIG70_11990"
     CDS             1256..2062
                     /locus_tag="VIG70_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8251317.1"
                     /translation="MSKPSPEIDRRAARRRFERTARTYEKASRLEDEVAARMLERLDY
                     MKIAPRRILDAASGPPRLALGKRYPHAEVIALDFAVEMLRAGKRKLFGKTPARVCGEL
                     ERLPIAADAVDFTWCNMALHWLSDPLPAFREFARVLAPEGLLMFSALGPDSLKELRAA
                     AGASRVHAFIDMHDLGDMLLGAGFSNPVMDMEIIEVDYTKGGSLLADLRLSGQTSARA
                     DRQRGLSGRGFGKRLGAPSRATFEVVYGHAWKSRKIEPEAKTVRVFKRMP"
     gene            2100..2540
                     /locus_tag="VIG70_11995"
     CDS             2100..2540
                     /locus_tag="VIG70_11995"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEY8251318.1"
                     /translation="MSTSHFSLVLKRNCSMSPKALAGVFAALALLVLAIGAGFAALGA
                     WLILPFAGLEVLLLGAAFVLYARHAADYEKIELESGRLDVEVAEGERTARYRMEGASV
                     VVEEGRVLLRDAKEQLEIGRHLGAEARAELAAELKMRLNQGTTT"
     gene            2537..3664
                     /gene="coxB"
                     /locus_tag="VIG70_12000"
     CDS             2537..3664
                     /gene="coxB"
                     /locus_tag="VIG70_12000"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY8251319.1"
                     /translation="MKGSKLVSLFAGLAAAAASGAAHAAAWYFQTPASKMAQEIDWLH
                     QVVMWIILVIFVGVFGFMFYACYAHRKSIGHKAEQFHENTTVEILWTVIPALILIVIA
                     WPVTKVVIAQKDTSAPDLTIKVTGYQWKWGYDYLRGEGEGISFVSTLATPREQIEGHA
                     PKGEHYLLEVDNEMVVPIGKKVRIITTAADVIHAWWIPALGAKQDAIPGFLRDIWFRA
                     EQTGTYRGQCVELCGKEHGFMPIVVRVVSNEDYSKWVGEQKKAMAAAADDPGKTWEPK
                     ELVARGERVYAANCVACHQASGQGLAAMKAPALANSKFVTGAEEGPIDTVLNGRPNTA
                     MQAFGKQLSDTDIAAVITYARNSWGNKASDVQPAEVKARRK"
     gene            3668..>4908
                     /locus_tag="VIG70_12005"
     CDS             3668..>4908
                     /locus_tag="VIG70_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY8251320.1"
                     /translation="MSSVAHDHAHPHDHTHDDHGHGHHGPYGGIMRWITTTNHKDIGT
                     LYLWFSFIMFSIGGLMAVGIRMELFSPGLQVMDPDFFNQLTTMHGLIMVFGAIMPVFV
                     GFANWQIPMMIGAPDMAFARLNNWSFWLLPFAATLLVLSFFMPGGAPGVGWTLYAPLT
                     VQMGIGMDLTIFAVHILGMSSILGSINIITTILNMRAPGMTLMRMPLFVWTWLITAYL
                     LVAAMPVLAGAVTMTLTDRHFGTTFFNAAGGGDPVLYQHIFWFFGHPEVYIIALPGFG
                     VISQVIPAFSRKPLFGYASMVYATSAIAIISFIVWAHHMYTVGMPVTGQLFFMYATMI
                     IAVPTGVKVFNWLFTMYGGRVRFKTPLLWSIGFMVTFVIGGMTGVLMAVPPADFVLHN
                     SLFLVAHFHNVIIGGVLFGAFA"
BASE COUNT          803 a         1619 c         1636 g          850 t
ORIGIN      
        1 accgagcagg cgctcgcgcg catcgccgac ccggcgggcg aaggcaagcg cgctttcatc
       61 aaggtctatg ccgaaggcgc gctcgctgag gccgactttt ccgaccggct gcgcaaggca
      121 ggcgtgcggc gctctcctgt cgacgggctg ccggtttccc tgaaggacct gttcgacgtg
      181 gcgggcgatc tgacccgggc cgggtcgagg atcatggaaa agaatccacc ggcaaacgca
      241 gatgcccttg cggtggcccg cttgcgcgcc gcgggcgcgg tgttcgtcgg ccgcaccaac
      301 atggtcgagt tcgcgttcgg cacgacggga atcaacccgc attacggcac gccgaggaat
      361 ccgtgggacc gcaagaccgg caggattccc ggcggctcgt cttccggcgc cgcggtcgcg
      421 caggccgacg gcatgtgcgt gatggcgctc gggtccgata cgcgcggatc gatccgcatc
      481 ccggcggcgt tctgcggcgt gaccggcttc aagccgacca cccggcgcgt gccgcgcgaa
      541 ggcgcgttcc cgctttccac cacgctcgac tcggtcgggc cgctcgccag ctccgtcgcc
      601 tgctgcgcgg tctacgacgc gatcctcgcc ggggagccgc ccgagccgct gtccgagctg
      661 cccgcgaagg gattgaggct cctcgtgccg aagaccgaag tccgcgacgg cctggacccg
      721 gaagtggctt ccgctttttc ggcggcgctt gaaaagctgg gtcgtgcagg ggcgtcgatt
      781 gcggaaaagc cggtgccggc gttcgaccgc cagggcgagt acttcaaggg cggcggcttc
      841 gccggcgccg aggcctacgc gattcaccgc aagaacgccg agcgcctcgc cgaatacgat
      901 ccgcgggtcg cgaagcgcgt gatcctcggc aaggacttga gcgccgcgga ttacgtcgac
      961 ttcgtgcggc tgcgcgccga gttccagcgc gaggtcgaag ccctcgtcgc gcctttcgac
     1021 gcgtttctcc tgccgacgaa cccctgcatc gcgccgccga tcgccgaggt cgacgccacc
     1081 gacgaggcgt acttccgctg gaacatgcgc atcctgcgca tcgtggggct ggtgaatttc
     1141 ctcgacggct gcgctgcgtc gctgccctgc cacgcgagcg gcgcggcgcc cgtcggcttg
     1201 atggtgtgcg ggccggcgat gagcgaccgg cgcgtcctcg cggtagcggc tgcggttgag
     1261 caagccctcg ccagaaattg accggcgcgc cgcgcggcgg cgcttcgagc gaaccgcaag
     1321 gacctacgag aaggcgtcgc gcctggaaga cgaggtcgcg gcgcgcatgc tcgagcgcct
     1381 ggactacatg aagatcgccc cgcgacggat cctcgatgcg gcgagcggtc cgccgcgcct
     1441 cgcgctcggc aagcgctatc cgcacgccga ggtcatcgcc ctggatttcg cggtggagat
     1501 gctgcgcgcg gggaaaagga agctctttgg aaaaaccccc gcccgggtgt gcggcgagct
     1561 ggagcggctg ccgatcgccg ccgacgccgt cgatttcacc tggtgcaaca tggcgctgca
     1621 ctggctctcg gatccgctgc ccgcgttccg cgagttcgcc cgcgtcctcg cgcccgaggg
     1681 cctgctcatg ttcagcgcgc tcggtccgga ttcgctgaag gagctgcgcg cggcggccgg
     1741 cgcgtcgcgc gtgcacgcgt tcatcgacat gcacgatctc ggggacatgc tgctcggcgc
     1801 tggcttctcc aacccggtca tggacatgga aatcatcgaa gtcgactaca ccaagggcgg
     1861 cagccttctg gccgacctgc gcttgagcgg acagaccagc gcgcgcgccg atcgtcagcg
     1921 agggttgtcc ggcagaggct tcggcaagcg gctgggcgcg ccctcacgcg ccacattcga
     1981 agtcgtttac ggccacgcgt ggaagtcgcg aaagatcgaa cctgaagcga aaaccgtgcg
     2041 tgttttcaag cgcatgcctt gaggcgtgcg ttccggatgt gatactttca gccccctagg
     2101 tgagcacgtc ccacttcagc ctggtcctga agcgcaattg ctcgatgtcg ccgaaggctt
     2161 tggcgggggt gttcgcggcg ctggcgctcc tcgttctcgc catcggggcg ggatttgcgg
     2221 cactgggcgc gtggctgatc ctgccgttcg cgggactgga agtgctgctg ctcggcgcgg
     2281 ctttcgtgct gtatgcgcgc cacgcggcgg actacgagaa gatcgaattg gaatcgggca
     2341 ggctcgacgt cgaggtcgcc gaaggcgagc ggaccgcgcg ctaccggatg gagggggcaa
     2401 gcgtagtcgt ggaagaaggg cgcgtcttgc tgcgcgacgc gaaagaacaa ctagagatcg
     2461 gccggcatct cggcgccgaa gcgcgcgccg agctcgccgc cgagctcaag atgagactca
     2521 atcagggaac cactacatga agggcagcaa actcgtttca ctgttcgccg gcctggcggc
     2581 agccgccgcc tcgggcgcgg cgcacgcggc cgcgtggtac ttccagacgc cggcgtcgaa
     2641 gatggcgcag gagatcgact ggctccatca ggtggtgatg tggatcatcc tggtgatctt
     2701 cgtcggcgtg ttcggattca tgttttacgc ctgctacgcg caccgcaagt cgatcggcca
     2761 caaggccgag cagttccacg aaaacaccac cgtcgagatc ctctggaccg tgattccggc
     2821 gctgatcctg atcgtgatcg cctggccggt gaccaaggtc gtgatcgcac agaaggacac
     2881 ctccgccccc gacctcacca tcaaggtcac cggctaccag tggaaatggg gctacgacta
     2941 tctgcggggc gagggcgagg gcatcagctt cgtgtcgacg ctcgccacgc cacgcgagca
     3001 gatcgaaggc cacgcgccca agggcgagca ctacctgctc gaagtcgaca acgagatggt
     3061 ggtgccgatc ggcaagaagg tgcgcatcat caccaccgcc gccgacgtga tccacgcctg
     3121 gtggattccc gctctgggcg cgaagcagga tgcgatcccc gggttcctgc gcgacatctg
     3181 gttccgcgcg gagcagaccg gaacgtatcg cggccagtgc gtcgagctgt gcggcaagga
     3241 gcatggcttc atgccgatcg tggtgcgggt ggtgtccaac gaggactact cgaaatgggt
     3301 gggcgagcag aaaaaggcca tggccgccgc ggccgacgat ccgggcaaga cctgggagcc
     3361 gaaggagctc gtcgcgcgcg gcgagagggt ctacgccgcg aactgcgtcg cgtgccacca
     3421 ggcgtccggc cagggcctgg cggcgatgaa ggcgcccgcg ctcgcgaact ccaagttcgt
     3481 caccggcgcc gaagaggggc cgatcgatac ggtgctgaac ggccgcccga acaccgcgat
     3541 gcaggcgttc ggcaagcagc tctccgacac cgatatcgcg gcggtcatca cgtacgcgcg
     3601 caattcctgg ggcaacaagg cgagcgacgt tcagcccgcc gaagtcaagg caagaaggaa
     3661 atagctcatg agctcagtcg cacacgatca cgcccacccg cacgatcaca cccacgacga
     3721 tcacggccac ggccaccacg gcccgtacgg cgggatcatg cgctggatca ccaccaccaa
     3781 ccacaaggac atcggcacgc tgtacctgtg gttcagcttc atcatgtttt cgatcggcgg
     3841 gctgatggcg gtgggcatcc gcatggagct gttctcgccc gggctgcagg tcatggaccc
     3901 ggacttcttc aaccagctca ccaccatgca cggcctgatc atggtgttcg gcgcgatcat
     3961 gccggtattc gtcggtttcg cgaactggca gatcccgatg atgatcggcg cgcccgacat
     4021 ggcgttcgcg cgcctcaaca actggtcgtt ctggctgctg ccgttcgccg ccacgctgct
     4081 ggtgctgtcg ttcttcatgc ccggcggcgc gcccggcgtg ggctggaccc tgtacgcgcc
     4141 gctcaccgtg cagatgggca tcggcatgga cctcaccatc ttcgccgtgc acatcctcgg
     4201 catgagctcg atcctgggct cgatcaacat catcactacc atcctcaaca tgcgcgcgcc
     4261 cgggatgacg ctgatgagaa tgccgctgtt cgtttggacc tggctgatca ccgcctacct
     4321 gctggtggcg gcgatgccgg tgctcgccgg cgcggtcacc atgacgctca ccgaccgcca
     4381 cttcggcacc accttcttca acgccgcggg cggcggcgac ccggtgctct accagcacat
     4441 cttctggttc ttcggccacc ccgaggtgta catcatcgcg ctgccgggct tcggcgtgat
     4501 ctcgcaggtg atcccggcgt tctcgaggaa gccgctgttc ggctacgcct cgatggtgta
     4561 cgccacctcg gcgatcgcga tcatctcgtt catcgtctgg gcgcaccaca tgtacaccgt
     4621 cggcatgccg gtgaccggcc agctgttctt catgtacgcc accatgatca tcgcggtgcc
     4681 gaccggcgtg aaggtgttca attggctgtt cacgatgtat ggcggcaggg ttcggttcaa
     4741 aacgccgttg ttgtggtcga tcgggttcat ggtgacgttc gtcattggcg gcatgaccgg
     4801 cgtcctgatg gcggtgccgc ccgccgactt tgtcctgcac aacagcctgt tcctggttgc
     4861 gcacttccac aatgtgatca tcggcggcgt cctgttcggc gcgtttgc
//
LOCUS       DATBSZ010000188        23850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1467090, whole genome shotgun sequence.
ACCESSION   DATBSZ010000188 DATBSZ010000000
VERSION     DATBSZ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23850
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467090"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4261)
                     /locus_tag="VIG70_12010"
     CDS             complement(<1..4261)
                     /locus_tag="VIG70_12010"
                     /inference="COORDINATES: protein motif:HMM:NF025611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HEY8251321.1"
                     /translation="MVVDGSLGDQSGQFSGSEVLVLDQNRDGLEQITQALAARSGSVG
                     ALHIVSHGSSGSLALGAATLDAASLESYQAQLRAWADALTADADILFYGCNLAADEAG
                     IGFVDELSRLTGADIAASTDLTGSAGLGGDWDLEYATGSIEADAMAASGDFVLATINY
                     DATTDTLTFTADAGQADSVTITSPAGGNQLQIVVANGDTIVLTGDTGSGFTLNGTSTQ
                     LNITNTSAAGTPISVFSASLLDLNDSFSFSNTQAQANGLGNLSFDGGSGNDTFSLLNN
                     QLNVLGSVSITGGGDNDTVTLNTTRIGGNLTLGTEVVTVSASATVDAGTNVINASGAG
                     VTFSAASTLTAATFTAGSLNIGGGIVTLDAGTTAGALSLSGSGNLSGTGNVTVTGATT
                     WSAGTMSGTGSTITQGALTISGLVGMDAGRLLDVRGGADWIAGQINLNTGSTGGAGSV
                     FNRAGSTFETSFDGVMLTGTFGGEVGPASLFTNEGIFRKTGGAGQTELRTPFINSNVV
                     EVLSGTLNLTDGGTHSGSFSGTGTLQFAGATDTHTLAATSSVTTNNVNFTSGTTNVGG
                     VYNIAGTTRVNGGTANFNSALTSLGTTLIVSSGAANLGTNNATVTTFTQSGGTLSGTG
                     NLTVTGVTTLSGGTMTGLGDTITQGLLTISGLVGLDAGRLLDVQAGANWTAGQINLNP
                     SSTGGAGSVVNRAGSVFATSFDGLMLTGTFGGEVGPASLFTNEGIFRKTGGTGQTELR
                     TPFNNSYVVEVLSGTLNLTDGGTHSGSFSGAGTLQFAGTDTHTLEAASSVTTNNVNFT
                     SGTTNVGGVYNIAGTTRVNGGTANFNSPLTSLGATLIVSSGAANLGTNTATVTTFSQS
                     GGTLSGTGNLTVTGVTTLSGGTMTGSGDTITQGALTISGLVGLDAGRLLDVRGGANWT
                     AGQINLNPSSTGGAGSVLNRAGSTFATSFDGLMLTGTFGGEVGPASLFTNEGIFRKTG
                     GTGQTELRLPFNNSNVVEVLSGTLSLTDSGTHSGSFSGAGTLQFAGSSAETHTLEATS
                     SVTTSNANFTSGTTNIGGVYDIAITGTTRVNGGTANFNSTLTSLGATLIVSSGAANLG
                     TNDATVTTFTQSGGTLSGTGDLTVSGASTLSGGSMTGSGDTITQGLLTINGSISLDAG
                     RLLDVQGGADWTAGQINLNPTSTGGAGSVVNRAGSVFATSFNGLMLTGTFGGEVGPAS
                     LFTNEGIFRKTGGTGVTELRTPFDNTNAVEVQSGTVNLSQGGTHTGSFATAAGTTLQF
                     GGGTHNLNTATAVSSLGRLLISSGTVNTTGTLTSGGLLENTGGALNAGGAINTATYSQ
                     SAGTLSGAGDLTVSGASTLSGGSMTGSGDTITQGLLTINGSIGLDAGRLLDVQSGASW
                     TAGQISLNTSIAAGSGTIINRAGSVFDNSFNGLMLTQSIGAD"
     gene            4557..4811
                     /locus_tag="VIG70_12015"
     CDS             4557..4811
                     /locus_tag="VIG70_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251322.1"
                     /translation="MRIDRESLASALAHLERGDWQAAHEIVQRDEESALACWAHGIVH
                     LMEGDAANARYWYRKAKRPFAADIPAEIAALSEALKRRTG"
     gene            complement(4780..8508)
                     /locus_tag="VIG70_12020"
     CDS             complement(4780..8508)
                     /locus_tag="VIG70_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8251323.1"
                     /translation="MIREPHFKSRRRESREPVRILLLEDDPISVELVRAYLGCIPFAE
                     AELQSAATVADALALLARGQVDLVISDLNLPDSEGAATIARMVQAATCPVIAITSDED
                     PGLRDAVLARGAYDFVQKGDLTEPTLQRLVRLAAMQARTFHTLRESEARFRGLTALLS
                     DWFWETDAEHQFVAAPGRGRVTAVTGLAAVAYVGKPRWQVPGLSPVGADWSAHRAALA
                     RRETFRDLELEQVRPDGSRCYLQISGEPVYREDGKFKGYRGTAKDVTQHRSAEAALRE
                     SEERFRQTFELAGSGMAHLGLDGRFLRVNRSLCRILGYPEAELIGRSAKDISHPADRD
                     VTDAARQHVRMGEADWIRAQKRYLRKDGATVWVELTVALARDAESNPIYEIAVIEDIT
                     ERKNAESALRESEARFRTLSELNSDWYWEQDAELRFVGTSGTSDARGGITPEAHVGMR
                     RWELPRTEIVGQPWDEHRRALAAREPFTDLLLRRTSTSGEVHYVSVAGRPIFNAQGSF
                     TGYHGVAKDVTNRIESELALRRFRAALDAAGDMVFLVDARTSTYVDFNETACRTLGYR
                     RDEMLGMDTQRVRMDRTREELAEDYRALVDAAPGASTTSVGTYRRKDGSTFPVEVTRR
                     LLETRDGGVVVATARDLTERRRAEQSQAAHHRYQERIARFGQSALVKSEPSELMKKAV
                     QSLLEALVAETVAYLEPESHTGELVLRAVVGVAGAAPGSVAGKPGEPIRQVLASGNRL
                     LTEGAKLPLAWAAELKSVALVPVRSDDKVRGVLCVGYRQPNAFGTEEVNFIEATASVL
                     STALQRIDSEVRLAYLAQFDSLTGLPNRALFADRFSQMIVQAARRSLPLAVLFIDLDE
                     FKLVNDTLGHAGGDALLKEVAVRLQSTVRGGDTVARISGDEFAIVLADLARPEDAALV
                     AQKVIDRLSAAVDVHGKEVFVTASVGIAAFPADGADAEALIGAADAAMYRAKQSGRNA
                     YQFFTAEINQRSRARAQMGSELRRALEREEFALAYQPKYHLATRRLSGAEALLRWKHP
                     ERGMVMPAEFIPVLEETGLIVPVGEWVVRRACADLKAWRDAGLGVGPVSVNLSARQFR
                     QADLHERIKSAVVSAGVDAGLLELEITESQLMQDPDHAIHVMRALCDAGMRIAIDDFG
                     TGYSSLAYLTRFPVGSLKIDRSFVKDMSSDKGDGTIVRTIIEMAHSLGFTVIAEGVET
                     EEQANFLRLLRCEQAQGYLFARPMPAGEFSRFCASAPR"
     gene            complement(8615..9520)
                     /locus_tag="VIG70_12025"
     CDS             complement(8615..9520)
                     /locus_tag="VIG70_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY8251324.1"
                     /translation="MSSKPLLPQHNRYDFVPLPQRKDYSWPDGKRLAFLLTTNVEWFA
                     FGAGLGHDPAKLGEPQTHRNYSWRDYGNRIGVWRLLELFDELGLPAAHNANSLLYEYA
                     PQVMAAIRKRGDEVVAHGRTNAENLRGLWQPDEERILREVTETIARQEGAPPAGWMGS
                     GAYETAYTPDLLKELGYKYLMDWPMDDQPVWLRTRAGPILSVPYPIELNDAQVIIHRR
                     QDASDFCDMIVDQFDEMVEQSERHPLVMNVSVHTYVFGQPFRLRRLRAALKHCRGHPG
                     AARVWWTRPRDLAEYCYSLAPGLVP"
     gene            complement(9517..10479)
                     /locus_tag="VIG70_12030"
     CDS             complement(9517..10479)
                     /locus_tag="VIG70_12030"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8251325.1"
                     /translation="MPASTEFVRAGNWVFGTGLRGAASPELFRRMEAGLVAAGSSMSR
                     VARLDQYYPDVSCVATYQAARKQAFARRQVAPSTSVVVSKLQDAASQVDLQIIAATAA
                     SGYVPVEVQTGLNRPDSSGYAPCLRVGDLVFVAGQLARDASGGLAAHGVRAETEYLLQ
                     RRLVPALDAAESGLDLVLKAQVYVSQPADTAAFWEVWKQVFGDGAPPTTVVPVRHPAF
                     LTREATVEVNAIAVHRSARSRVREIEGQARMLDGLLFVRGLTSPSALAQAKDIFAAAG
                     TDLSQVVRAHFFHSKGAAPTDPGFPYSAFEVPAELTVDLWGYAT"
     gene            complement(10463..11416)
                     /locus_tag="VIG70_12035"
     CDS             complement(10463..11416)
                     /locus_tag="VIG70_12035"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8251326.1"
                     /translation="MKRAILVFACLAAGAACAQEYPSRPIRVVVPYSAGGSSDGPMRV
                     IAQEMSKQLGQQMVVENKPGQGAMLGSAEVVKAPADGYTLLLASNPNAISASLYSRLT
                     HDPVEDFAPISLLASEQAVLLVNPSVPAKTLREFIAYAKERPGKVDYSSSGNGSAQHL
                     FMAMFATMAGIQLNHIPYKGSAQAVTDLLGGQVSAAMPGLAAMRGHIREGRARALAVT
                     GARRASAAPEIPTMIEQGLKDYELYVWMGMLAPKGTPAPVIERLHREFTTALRAPAVK
                     TFMDGAAIEAVGSTPAQFGAFFREEKVRWAKLIKETGARID"
     gene            complement(11413..12255)
                     /locus_tag="VIG70_12040"
     CDS             complement(11413..12255)
                     /locus_tag="VIG70_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY8251327.1"
                     /translation="MVERRDYSWPGGKRLAFLLTTNIEWFAFGAGLGNDPAKPGEPQT
                     HRNYAWRDYGNRIGIWRLFDLLDELGLPAGHCTNSLIYEYAPQITGAIRRRGDEVIAH
                     GRTNAENLRGLWQTDEERILREVTDTIARHEGAPPSGWMGSGAYETAHTPDLLKELGY
                     RYVMDWPMDDQPIWMRTRAGRILSIPYPVETDDAQGVIHRRWSAATFADAVVDQFDEM
                     ARQCEAHPLVMNVSVHPYVFGQPFRVRQLRRALRHCAGRAAVWYCRPRDIADYCYSNG
                     ESIQ"
     gene            complement(12294..13250)
                     /locus_tag="VIG70_12045"
     CDS             complement(12294..13250)
                     /locus_tag="VIG70_12045"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8251328.1"
                     /translation="MRRAMCLVLLFGGTALAQSEYPTKPVRLVVGFAAGGISDVLGRA
                     VAIPLSKQLGQQVIVENKPGAGTTIAGDFTVKSAPDGYTLWLQDITTHAINANLYAKL
                     PYDSMKDFSFVAMVASTPLMLVVHPSTPANNVRELIALLKANPGKYSYGSSGNGTIVH
                     LSSEMLKSSAGVDALHVPYKGSNPATAAILGGEVAFVFSTMPPAISNVRGGKLRALAV
                     TTPKRSGAAPEVPTMIESGLPNFEIVLYSGVLAPKGMERALVRRINGEFARAVQAPEM
                     QPVWRNIGADPIATSPEEFERQTAAEIAKLAPVVKASGAKVD"
     gene            complement(13247..14167)
                     /locus_tag="VIG70_12050"
     CDS             complement(13247..14167)
                     /locus_tag="VIG70_12050"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8251329.1"
                     /translation="MTPDGRTPVVVVTGFLGSGKTTLVNKLVRFSDSLVIVNEFGEIP
                     VDHVLVEGTTEKAVALPNGCLCCHLRGDLEETLIGAAMRRRRGDIAGFDRVIVETSGL
                     ADPGPVAQTLYADQALARDFKLGPIVTVVDSFDLESRKVSAEIARAQIAAADVVVLSK
                     VDRVGDPTVAEAEIRALNPAAQCIRASYGEADPRVILEAKPGTAPVFAAAAHDSRIRS
                     FALRFEQPVRRGLLQRFLSTLVDLRGADLLRVKGIVPVESGLVLVQGVRHVFDRLRPV
                     EGGEPALVFITNGVEKSEVEALWRAMKGLS"
     gene            complement(14164..15369)
                     /locus_tag="VIG70_12055"
     CDS             complement(14164..15369)
                     /locus_tag="VIG70_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251330.1"
                     /translation="MFLNMKLLAQHTLNGFGGMGEGTSMQLARDGRRVLWLAHESAPK
                     NFTAVDVSDPRAPKVIVQTDLPHAQMRSNSLEVAGDLMAVAYQVTKFGLKPAGFELFD
                     ISVPEKPKSISFFDASGPHSRGCHQLWFVDGSTVHMACADPELKPRNPKDDQIYRIVD
                     VANPSKPVAVGRWHLPGTMESDDAPPPQRLPAKFDAGFRAHNTNVYPERPDRCYLAYI
                     DGGIMVLDISDRARPKLLSHWTNSPPYNGFNHTALPFFGRGLLVVSDESVKDNADDWP
                     KLVWLLDARDERHLVSIATLPMPPVDAFKSRGGRFGAHNLHENPPVPGAWRSEDIVLG
                     TFFNGGVRAYDVTDPYSPREVAYFVPPTPERAKTGAVQLNDVFVDDRGIVFTVDRGGG
                     GLYCLEMNV"
     gene            complement(15374..17725)
                     /locus_tag="VIG70_12060"
     CDS             complement(15374..17725)
                     /locus_tag="VIG70_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8251331.1"
                     /translation="MRLRSLQSRIVLFFVALLVVVQGITLLLVNAANERNARDNVRHE
                     IVVGEKIFRRLLEQNHARLTQAALIVSLDFAFREAAATRDVSTIESVLVNHGSRIRAD
                     TMALVSLERQLIADTFDRRRAGQPFPFTRLLEAAERDGSAAGMVLVDGAAFQVAVVPV
                     RAPTPIAWTVVGFTVDDRLARDLANLAALEVTFVSREGQVWRVLGSSADSELQEAVLR
                     TLPKGNAAGDRAMPLDLGGREYESFVMALDGSDSRIVAVLARSLDEAPYRRLGPLLVL
                     LVAGSIVASIVGSVLIARSVTRPLSLFSAMTKRIEEGDYAQAIEAEGTDEVSELARRF
                     NLMREAIAAREEQILKLAYRDPLSDLPNRVLFNDRLRVALEGAKRGGAALTVLVMDLD
                     RFKYINDTLGHHVGDMVLQQVALRITRVVRKSDTTARLGGDEFAVLLVNSGVDEARGV
                     AQKVVAALEAPIAIGQHSLDVRASVGIAAFPAHAGDADTLMRRADAAMYSAKRGNTGF
                     AVYDAHVQEQREEQLSLLSELRQAIDRGQLRLVYQPKIDLASGILSGVEALVRWAHPA
                     KGLITPDRFIPFAEQTGFIKTITAWVIDTAVRQGAAWRMAGRPTPISVNISAQDLLNP
                     ELSGLLTAALERHRLPPELLCLEITESGVMQDAARAIEVLKRLEKLGVKRAIDDFGTG
                     YSSLSYVKQLSVDELKIDRSFLRGMLTDMRDRAIVLSTIDLAHNLGLIVVAEGVEEQE
                     AADLLRALGCDQIQGTLVSRPLEVTALETWLFTRSAAPVSRIS"
     gene            complement(17740..18372)
                     /locus_tag="VIG70_12065"
     CDS             complement(17740..18372)
                     /locus_tag="VIG70_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HEY8251332.1"
                     /translation="MLAAVLALAPLAHAADLRATVRDDQGRAVPDAVVVAVPQDAAPQ
                     EGRSRVEQVEQIDLEFVPHVKAIQTGTAVSFPNRDDVRHHVYSFSPAKRFELPLYVGT
                     PAQRVVFDQPGVVAIGCNIHDWMVGYLYVSPSPHFATTGAQGTARIAGLPAGRYTVHV
                     WHPRLAATEETTRRPAELGAEGNAALEWVVALKPETRVRRAPVSRRGARY"
     gene            complement(18392..19252)
                     /locus_tag="VIG70_12070"
     CDS             complement(18392..19252)
                     /locus_tag="VIG70_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251333.1"
                     /translation="MRTALALVAAALPAAAAAHWTLDADLARTYDDNLSRAQRESDIV
                     RDHALALRGALGRQLVVAGGDLVLRAEARGALYDDFPGLDHAALGAGAAWRRKLGLGL
                     TAPWLAADAAFFGEDYSEPVRDGNRAALALELGKRLGPSFEVSLGAAYDRRWQSEDFG
                     TVPGISGKPFSLQGRSLSVRGSYAFGEKLLAFGALAARHGDVASSTRRNQQIFRESAA
                     IAADPAFGPDYIAYKLTGARTDSYTLGLSWALGVNASVDAALTSERTSARGGLDYDAN
                     LYSVSLVYRD"
     gene            19308..20282
                     /locus_tag="VIG70_12075"
     CDS             19308..20282
                     /locus_tag="VIG70_12075"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8251334.1"
                     /translation="MKLPIAATLAGVLFSLFALSAGAQGWPAKPVRIVVAYPPGGGID
                     VLGRQLADKLAPLWGQPVVVENKPGANTIVATDAVAKSAADGHTILLTTDATFSINPH
                     LYAKLPYDAQRDFAPVTMLVLLQQLLVAHPSLPANSVAELVALAKAKPGAINYASYGS
                     GSQPHLAGEMLKNKAGIDLVHVPYKGISLAVPAVMAGEVQLTFAGIATSMPQLKAGRI
                     KALAIGGAARSPLLPQVPTFTELGYPEVETHAWFGLFVPAGSPREAVARIQQDTKRIL
                     DEPDFRQKQLVDKGYEVVGSASQDFVMYLRTDSESRGRAVRISGAKAE"
     gene            20292..21065
                     /locus_tag="VIG70_12080"
     CDS             20292..21065
                     /locus_tag="VIG70_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8251335.1"
                     /translation="METVFVEPAPRLAVSVAGEGELVLFLHGIRGNRRNWAAQIEFFS
                     RRFRAAAWDARGYGDSDDYEGALQFEYLTGDVMRVAEHFKARKLHLVGLSMGGRIARN
                     VALRAPERLHSLVLISTNPGFDAMSPESVKRFVNERRTRTPQTLAKLLGSRASRAAYD
                     ELLDSVARIHDASYQKTLEASVAQDRAAPIEQIRVPTLVIAGEEDTVYPAELAEQMAR
                     RIPGAELLMLERTGHLANLEQPDRFNKAVFDFVSRHSAR"
     gene            21100..21855
                     /locus_tag="VIG70_12085"
     CDS             21100..21855
                     /locus_tag="VIG70_12085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8251336.1"
                     /translation="MASAKRVALVTGAGSGIGKSAALALLKDGYHVALVGRRKDMLEK
                     AAAESGAKDRALVLAADITKDEQVKDVFAKTKSAWGRLDVLFNNAGIGAPAVPMEDLP
                     IEKFREVVNINLVAMFMCTQEAIRIMKAQDPKGGRIINNGSISAHAPRPMSVAYTSTK
                     HAVTGLTKCISLDGRKNDITASQIDIGNALTELAARMANGVPQADGSIKPEPLMDVKH
                     VGQAVLHMANLPLDVNVQFMTIMATKMPFVGRG"
     gene            complement(21852..>23850)
                     /locus_tag="VIG70_12090"
     CDS             complement(21852..>23850)
                     /locus_tag="VIG70_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8251337.1"
                     /translation="GGQNGLMNLPRPALFGFAGSERTVAVVAIVTAACALLVFRWLSL
                     GAWGAAMRAVRDSDVAAESVGIDPLTVKTVAFAGSAALAGLAGGLFAPLSGFVTPSSF
                     AFLQSILFVLVVMIGGAGYVAGPLVGAAIVVLLPEFLAALAEYRLLFFGALLLVVLWL
                     APEGVVGTVLRLVSKRRAPRPAAAAAAPIAPRAKAALAVRGLGIAFGGVRAAHDIAFE
                     AQPGEVTSLIGPNGAGKTTVLNMLSGFYRPAAGSIRIGGEALEGRPAWRIARSGVART
                     YQTTQLFGSMSVAENLAIASGDESLAAFVGYGGDMDARAADLAHVDKRLVEIARALAT
                     RPAVLLLDEPAAGLSVHDKAKLAGLLRRIADAGIAVVLVEHDMSVVMGISDRVVVLDA
                     GLRIASGTPAEVQRDPAVRKAYLGEEQQRLVAKRAEPGAPLLEVDRLAAGYGAEPVLK
                     GIDLGVRQGEMVAVLGANGAGKSTLMRALSGLHRPVAGGIALAGEELATQPAHRVVAR
                     GVVLVPEGRQVFAELSVLDNLLLGAFSRKKVDRAEIDAVLERFPRLRERLQQRAGLLS
                     GGEQQMLAIGRGLMAKPRLLLLDEPSLGLAPQVANDLFAALDRLRAESATILLVDQMA
                     GLALALADRAYVIEGGRIVASGSAAEIAADGSLEKAYLAVT"
BASE COUNT         3716 a         8354 c         8001 g         3779 t
ORIGIN      
        1 tgtcagcccc gatgctctgc gtgagcatca acccgttgaa gctgttgtcg aacaccgagc
       61 cagcccggtt gatgatcgtg cctgagcctg ctgcaatgct cgtgtttaga ctgatctggc
      121 ccgcggtcca gctcgccccg ctctgcacat cgagcagccg ccccgcatcc agcccgatgc
      181 tcccgttgat cgtcagcagg ccctgcgtga tcgtgtctcc cgagcccgtc atcgacccgc
      241 ccgagagcgt gctcgcaccg ctcaccgtca gatcgcccgc ccccgacagc gtccctgcgc
      301 tctggctata agtcgccgta ttgatcgccc cgccggcgtt gagcgcgccg ccggtattct
      361 ccagcagccc gccgctggtc aatgtcccgg tcgtattcac cgtgccgctg ctgatcagca
      421 gccgccccag cgagctgacc gccgttgcag tgttcaggtt gtgcgtgccg ccgccgaact
      481 gcagcgtcgt ccccgccgcc gtggcgaagc tgccggtgtg tgtgccccct tgggagagat
      541 tgaccgtccc gctctgcacc tcgaccgcat tggtgttgtc gaacggcgtg cgcagctccg
      601 tcacgcccgt gccaccggtc ttgcggaaga tcccttcgtt cgtgaacagc gacgccggtc
      661 cgacctcgcc accgaaagta cccgtgagca tcagcccgtt gaaactcgtc gcgaacaccg
      721 accccgcccg gttgacgacg ctccctgcgc ctccggtcga ggtcggattc agattgatct
      781 ggcccgcagt ccagtccgca ccgccttgca cgtcaagtag gcgccccgcg tccaggctga
      841 tgctcccgtt gatcgtcagc aggccctgcg tgatcgtgtc tcccgagccc gtcatcgacc
      901 cgcccgagag cgtgctcgcc ccgctcaccg tcagatcgcc cgtgcccgag agcgtgccgc
      961 cgctctgagt gaacgtcgtc accgtcgcgt cgttggtgcc gagattcgct gcaccggagc
     1021 tcacgatcag cgtcgcccca aggctcgtga gcgtgctgtt gaagttcgcc gtgccgccgt
     1081 tgacccgcgt ggtccccgtg atagcgatgt cgtacacacc cccgatattg gtcgtaccgg
     1141 aggtgaagtt cgcgttgctg gtcgtgacgc tgctcgtagc ctccagcgtg tgcgtctcgg
     1201 cgctactacc ggcaaactgc agcgtgcctg ccccgctgaa gctgccgctg tgcgtgccgc
     1261 tgtccgtgag gctcagcgtc ccgctcagca cctccaccac gttgctgttg ttgaacggca
     1321 ggcgcagctc cgtctggccc gtgccaccgg tcttacggaa tatcccttcg ttcgtgaaca
     1381 gcgatgctgg ccccacctcg cccccgaagg tgcctgtcag catcagcccg tcgaagctcg
     1441 tcgcgaacgt tgaccccgcc cggttgagga cgctgcccgc ccctccggtc gagctcggat
     1501 tcagattgat ctggcccgca gtccagttcg caccgccccg cacatcgagc agccgccctg
     1561 catccagccc gacgagcccg ctgatcgtca gcgcgccctg ggtgatcgtg tcccccgatc
     1621 ccgtcatcgt cccgcccgag agcgtcgtca ccccggtcac cgtcaggttg cccgtgcccg
     1681 agagcgtgcc accgctctga ctgaacgtcg tcaccgtcgc ggtgttggtg ccgagattcg
     1741 ccgcaccgga gctcacgatc agcgtcgccc caaggctggt cagagggctg ttgaagttcg
     1801 ccgtgccgcc gttgacccgc gtagtccccg cgatgttgta cacgcccccg acattggtcg
     1861 taccggaggt gaagttcacg ttgttcgtcg tgacgctgct cgcagcctcc agggtgtgcg
     1921 tatcggtacc ggcaaactgc agcgtgcctg ccccgctgaa gctgccgctg tgcgtgccgc
     1981 cgtccgtgag gttcagcgtc ccgctcagta cttccaccac gtagctgttg ttgaacggcg
     2041 tgcgcagttc cgtctgacca gtgcccccgg tcttgcggaa gatcccttcg ttggtgaata
     2101 gcgacgccgg cccgacctcg ccgccgaagg tgcccgtgag catcagcccg tcgaagctcg
     2161 tcgcgaacac cgaccccgcc cggttgacga cgctccctgc gcctccggtc gagctcggat
     2221 tcagattgat ctggcccgca gtccagttcg ccccagcctg cacgtcgagc agccgccccg
     2281 cgtccagccc gacgagcccg ctgatcgtca gcaggccctg cgtaatcgtg tcccccaagc
     2341 ccgtcatcgt cccgcctgac agcgtcgtca ccccggtcac cgtcaggttg cccgtgcccg
     2401 agagcgtgcc gccgctctga gtgaacgtcg tcaccgtcgc gttgttggtg ccgagattgg
     2461 ccgcacccga gctcacgatc agcgtcgtcc ccaggctggt gagcgcgctg ttgaagttcg
     2521 ccgtgccgcc gttgacccgc gtggtccccg cgatgttgta cacacccccg acattggtcg
     2581 taccggaggt gaagttcacg ttgttcgtcg tgacgctgct cgtagccgcc agcgtgtgcg
     2641 tatcggtagc accggcaaac tgcagcgtgc ccgtcccgct gaagctgccg ctgtgcgtgc
     2701 cgccgtccgt gaggttcagc gtcccgctca gtacttccac cacgttgctg ttgatgaacg
     2761 gcgtgcgaag ttccgtctga ccggcgccgc cggtcttgcg gaatatccct tcgttggtga
     2821 atagcgacgc cggcccgacc tcgccgccga aggtgcccgt caacatcacc ccgtcgaagc
     2881 tcgtctcgaa cgttgatccc gcgcggttga agacgctgcc cgcgcctccc gttgagccag
     2941 tattcaggtt gatctggccc gcaatccagt ccgcaccgcc ccgcacgtcg agcagccgcc
     3001 ccgcgtccat cccgacgagc ccgctgatcg tcagcgcgcc ctgggtgatt gtgctgccgg
     3061 tgccgctcat cgtcccggcg ctccacgtag tcgcgcccgt caccgtcaca ttccccgtgc
     3121 cggacagatt tccgctaccc gacaagctca aggctcccgc cgtggtgccg gcatcgaggg
     3181 tcactattcc gccgccgata ttcagtgaac ccgcggtgaa cgtggccgcc gttagggtcg
     3241 atgcggcgct gaaggtcacg ccagcgcccg atgcgttgat gacgttagtg ccggcatcca
     3301 ccgttgcgct cgcagacacg gtcacgactt ccgtcccgag cgtcagattg ccgccaatcc
     3361 gggtggtatt cagggttacc gtgtcgttgt caccccctcc ggtgatcgac acgctgccga
     3421 gcacgttcag ctggttgttc aataaggaga acgtgtcatt gccgctaccg ccgtcgaaag
     3481 acaggttgcc gaggccgttg gcctgggctt gagtgttgga gaagctgaag ctgtcgttca
     3541 ggtccaggag agatgcacta aagaccgaaa tcggcgtgcc ggcggcggag gtattagtga
     3601 tgttgagttg cgtgctcgtt ccgttgaggg tgaagccact tcccgtgtca ccggtcaaca
     3661 ctatcgtgtc gccattggcg acgacgatct gcaactggtt gccccctgcg gggctggtga
     3721 tcgtcaccga gtcggcctgc ccggcatccg cggtgaaggt cagggtgtcg gtcgttgcgt
     3781 cgtaattgat cgtggcgagg acgaaatcgc ccgaagccgc catcgcatct gcctcgatcg
     3841 agccggtcgc gtattcgaga tcccagtctc cgcccaggcc ggcgctgccc gtgaggtcgg
     3901 tcgaggcggc gatgtcggcc ccggtcaggc ggctcaattc atcgacgaat ccgatccctg
     3961 cctcatcggc agccaagttg cagccgtaga acaggatgtc cgcgtcggcg gtcagcgcat
     4021 cggcccaggc gcgcagttgc gcctggtagc tctcgaggct cgccgcatcg agggtcgcag
     4081 ctccgagggc gaggctgccg ctcgagccgt gggaaacgat gtggagcgcg cccacgctgc
     4141 cggagcgtgc ggcgagggct tgcgtgatct gctcgaggcc gtcgcgattc tggtcgagga
     4201 ccagcacctc gctgccggag aactggccgg actggtcgcc gagcgagccg tcaacgacca
     4261 ccagcgatcc ggctgcggcg gggtcggccg acaggaggag ccggctttca agggactcga
     4321 aagcgaggcg tttgcgggga gggggcggtg gggaacggcg gcggtccgac ttgctggcga
     4381 aaatgcgagc gatactggat gcgatgacgc tacgccctgg ccgcatagac ccccccccac
     4441 tgtgttgcag gaatcttagt ccaaacctgt agaaaccggc atggctggac aagggaaata
     4501 aattacgcgg aggctgagca ttacggcacg cccggcgttt gctaccgtac ggcggcgtga
     4561 ggatcgatcg agaaagtctg gcgagcgcgc tggcccacct cgagcgcggc gactggcagg
     4621 ccgcgcacga gatcgtgcag cgcgacgagg aatcggcgct cgcctgctgg gcgcacggca
     4681 tcgtgcacct gatggaaggc gatgcggcga acgcgcgcta ctggtatcga aaggcgaagc
     4741 ggcccttcgc tgcggacatt cccgccgaga tcgcagccct cagcgaggcg ctgaagcgca
     4801 gaaccggctg aactcgccgg cgggcatcgg ccgggcgaac aggtagccct gcgcctgctc
     4861 gcagcgcagc aggcgcagga agttggcctg ctcctcggtt tcgacgccct cggcgatcac
     4921 ggtgaagccc aggctgtgcg ccatctcgat gatggtgcgc acgatggtgc cgtcgccctt
     4981 gtcgctcgac atgtccttga cgaacgagcg gtcgatcttg agcgagccca ccgggaaccg
     5041 ggtgaggtag gcgaggctcg aatagccggt gccgaagtcg tcgatcgcga tgcgcatgcc
     5101 ggcgtcgcac agggcgcgca tcacgtggat ggcgtgatcc ggatcctgca tcagctggct
     5161 ctcggtgatc tcgagctcga gcagccccgc atcgacgccg gccgacacga cggccgactt
     5221 gatcctttcg tgcagatcgg cttggcggaa ctgccgcgcc gacaggttca ccgataccgg
     5281 tcccacgccc aggccggcgt cccgccacgc cttcaggtcc gcgcaggcgc ggcgcacgac
     5341 ccattcgccc accggcacga tgagcccggt ctcctccagc accggaatga actccgccgg
     5401 catgaccatg ccgcgctcgg gatgcttcca gcgcagcagc gcctcggcgc cgctcagccg
     5461 ccgggtggcg aggtggtact tcggctggta ggcgagcgcg aattcctcgc gctcgagcgc
     5521 ccgcctgagc tccgacccca tctgcgcccg cgcgcgcgag cgctggttga tctcggcggt
     5581 gaagaactgg taggcgttgc ggcccgactg cttcgcccgg tacatggccg cgtcggcggc
     5641 gccgatcagc gcctcggcgt cggcgccgtc ggcggggaac gcggcgatgc cgacgctcgc
     5701 agtgacgaac acctccttgc cgtgcacgtc gaccgcggcc gagaggcggt cgatgacctt
     5761 ctgcgccacc aacgcggcat cctcgggccg cgccaggtca gcgagcacga tcgcgaactc
     5821 gtcgccgctg atgcgcgcca cggtgtcgcc gccgcgcacc gtggattgca ggcgtaccgc
     5881 cacctccttg aggagcgcat cgccgcccgc gtgcccgagc gtgtcgttca ccagcttgaa
     5941 ttcgtccaga tcgatgaaca gcaccgccag cggcaggctg cgccgtgccg cctgcacgat
     6001 catctgcgag aagcggtcgg cgaacagcgc ccggttgggc aatccggtca gcgaatcgaa
     6061 ctgcgcgagg taggcgagcc tgacttcgct gtcgatgcgc tgcagcgcgg tcgacagcac
     6121 gcttgccgtc gcctcgatga agttcacctc ctcggtgccg aaggcgttcg gctgccggta
     6181 gcctacgcac agcacgccgc gcaccttgtc gtcgctgcgc accggaacga gcgcgacgct
     6241 cttcagctcg gctgcccacg cgagcggcag cttggcgccc tcggtgagca ggcggttgcc
     6301 cgaagcgagc acctggcgaa tcggttcgcc cggcttgccg gccaccgacc ccggggcggc
     6361 gccggcaacg ccgaccaccg cgcgcaggac gagctcgccc gtgtgcgact ccggctccag
     6421 gtaggccacc gtctccgcca ccagcgcctc gagcagcgac tgcaccgcct tcttcatcag
     6481 ctcggaaggc tccgacttga cgagcgccga ctgcccgaag cgggcgatgc gctcctggta
     6541 gcggtggtgc gcggcctggc tctgctcggc gcgccggcgc tcggtgaggt cgcgcgccgt
     6601 ggcgaccacg accccgccgt ctcgcgtctc gaggagcctg cgcgtcacct cgacggggaa
     6661 cgtgctcccg tccttgcgcc ggtaggtccc tacgctggtc gtgctcgcgc ccggagcggc
     6721 gtccaccagg gcgcggtagt cctcggcgag ctcctcgcgc gtgcggtcca tacgcacccg
     6781 ctgcgtgtcc atccccagca tctcgtcgcg ccgatagccg agggtgcggc atgcggtctc
     6841 gttgaagtcg acgtaggtcg aggtgcgcgc gtcgaccagg aagaccatgt cgccggcggc
     6901 gtcgagcgcc gcgcggaatc tgcgcagcgc gagctccgac tcgatgcggt tcgtgacgtc
     6961 cttggcgacg ccgtgatagc cggtaaagga tccctgtgcg ttgaaaatcg gccgcccggc
     7021 gacgctgacg tagtgcactt cgccgctcgt cgaggtgcgc cgcagcagca ggtcggtgaa
     7081 cggctcgcgc gccgcgagcg cgcggcggtg ctcgtcccac ggctggccga cgatctcggt
     7141 ccgcggcagc tcccagcggc gcattccgac gtgggcttcc ggggtgatcc cgccgcgcgc
     7201 gtcgctcgtg ccgctggtgc ccacgaaccg cagctccgcg tcctgctccc agtaccagtc
     7261 cgagttcagc tccgagagcg tgcggaagcg cgcctcgctc tcgcgcagcg ccgactcggc
     7321 attcttgcgc tcggtgatgt cctcgatcac cgcgatctcg tagatcggat tgctctctgc
     7381 gtcgcgcgcg agcgcgaccg tcagctcgac ccagaccgtg gccccgtcct tgcgcaggta
     7441 gcgcttctgc gcgcgtatcc agtcggcctc gcccatgcgc acgtgctgcc gcgcggcatc
     7501 ggtcacgtcg cggtccgccg gatgcgagat gtccttcgcc gagcgcccga tgagctcggc
     7561 ctcgggatag ccgaggatgc ggcacaggct gcggttgacg cgcaggaagc gcccgtcgag
     7621 gccgaggtgc gccatccccg atccggccag ctcgaaggtc tggcggaaac gctcctcgct
     7681 ttcgcgcagg gccgcctcgg cgctcctgtg ttgcgtgacg tccttcgcgg tgccgcgata
     7741 gcccttgaac tttccgtcct cgcgatagac cggctccccg ctgatctgca ggtagcagcg
     7801 gctgccgtcg ggcctcacct gctcgagttc gaggtcccgg aaggtctcgc gccgcgcgag
     7861 cgcggcgcgg tgcgcgctcc aatccgcgcc caccggcgag aggcccggca cttgccagcg
     7921 gggcttgccg acgtaggcca cggccgcgag acccgtgacg gcggtgaccc gcccccgccc
     7981 cggcgcggcc acgaactggt gctcggcgtc ggtctcccag aaccagtcgg acagcagcgc
     8041 ggtcaggccg cgaaagcgcg cttcgctctc gcgcagcgtg tggaaggtgc gcgcctgcat
     8101 cgccgcaagg cgcacgagcc gctgcagcgt cggctcggtc agatcgccct tttgaacgaa
     8161 atcgtacgct ccccgcgcga ggaccgcgtc gcgcaatccc gggtcctcgt ccgaggtgat
     8221 cgcgatcacc gggcatgtgg cggcctgcac catgcgcgcg atcgtggcgg cgccctccga
     8281 gtcgggaaga ttcaggtcgg agatgacgag gtcgacctgg cctcgcgcca gcagcgcgag
     8341 agcgtcggca acggtggcgg cgctctgcag ctcggcttcg gcgaacggga tgcagccaag
     8401 ataagcgcgg acgagctcca cgctgatggg atcgtcttcg agcagcagga tgcgcaccgg
     8461 ctcgcggctc tcgcgccggc ggctcttgaa atgcggctcg cggatcaccg atccctcacg
     8521 cgcgcgagca tgccccctat cgggcgcccc ggccatcggg gtttcccgcg ggcttacaag
     8581 gctttacatg aaagcccgtt catcgcgctc caaatcaggg aacgaggccc ggcgccaggg
     8641 aatagcaata ctcggcgagg tcgcgcggcc gggtccacca aacgcgcgcc gcccccggat
     8701 gcccgcggca atgctttaag gcagcgcgca ggcggcgcag ccggaacggc tggccgaaga
     8761 cgtaggtatg caccgagacg ttcatcacca gggggtggcg ctcgctctgc tcgaccatct
     8821 cgtcgaactg gtccacgatc atgtcgcaga agtcggacgc atcctgcctg cggtggatga
     8881 tgacctgcgc gtcgttgagc tcgatgggat aggggaccga caggatcgga ccggcgcgcg
     8941 tgcgcagcca gaccggctgg tcgtccatcg gccagtccat caggtacttg taacccagct
     9001 ccttgagcag atcgggcgta tacgcagtct cgtaggcgcc cgagcccatc cagccggccg
     9061 gcggcgcgcc ctcctgccgg gcgatggtct cggtcacctc gcgcaggatg cgctcctcgt
     9121 cgggctgcca cagcccgcgc aggttctcgg cattggtgcg cccgtgcgcg accacttcgt
     9181 cgccgcgctt gcggatcgcg gccatgacct gcggcgcgta ttcgtacagc aggctgttcg
     9241 cgttatgcgc ggccggcagg ccgagctcat cgaaaagctc gagcagccgc cacacgccga
     9301 tgcgattgcc gtagtcgcgc caggaatagt tgcggtgcgt ctgcggctcg ccgagcttcg
     9361 ccggatcgtg tcccaggccg gcaccgaacg cgaaccactc gacgttggtg gtcagcagga
     9421 acgcgagacg cttgccgtca ggccagctgt agtccttgcg ttgcgggagc ggaacgaagt
     9481 cgtagcggtt gtgctgggga agcagcggct tgctgctcat gtggcgtacc cccacaagtc
     9541 gacggtcaat tccgccggca cttcgaaggc gctgtacgga aagccgggat cggtgggcgc
     9601 cgctcctttg gaatggaaga aatgcgcccg caccacctgc gagagatcgg tcccggcggc
     9661 ggcgaagatg tctttcgcct gcgccagcgc gctcgggctg gtcagcccgc ggacgaacag
     9721 caacccgtcc agcattcgag cctggccctc gatctcgcgc acgcgcgagc gcgccgagcg
     9781 gtgcacggcg atcgcgttga cttcgacggt cgcctcgcgc gtgaggaacg ccgggtgacg
     9841 caccgggacg accgtggtcg gcggcgcgcc gtcgccgaaa acctgcttcc agacctccca
     9901 gaacgcggcc gtgtccgccg gctggctgac atagacctga gccttcaata cgagatcgag
     9961 cccgctttcc gccgcgtcca gggcgggaac gaggcgacgt tgcagcaggt actcggtttc
    10021 ggcgcgcacg ccatgcgccg ccagcccgcc ggaggcgtcg cgcgccagct ggcctgcgac
    10081 gaagaccagg tcgccgaccc gaaggcacgg tgcatagccg gaagaatccg gccggttcaa
    10141 gccggtctgc acttccaccg gcacgtagcc gctggcggcc gtcgccgcta tgatctgcaa
    10201 atccacttgg ctcgccgcgt cctgcagctt gctgacgacc accgaagtgc tgggcgcgac
    10261 ctggcggcgc gcgaacgcct gcttgcgcgc ggcctggtag gttgcgacgc acgagacgtc
    10321 ggggtagtac tggtcgagcc gcgcgacccg ggacatcgag cttcccgccg ccaccagccc
    10381 ggcctccatt cgccggaaca gctcggggga agcggcgccg cgcagtccgg tgccgaacac
    10441 ccagtttccg gcgcgcacga actcagtcga tgcgggcacc ggtttccttg atgagcttcg
    10501 cccagcgcac cttctcctcg cggaagaaag cgccgaactg cgccggcgtc gagcccaccg
    10561 cctcgatcgc ggcaccgtcc ataaaggttt tcaccgcggg cgcgcgcagc gcggtggtga
    10621 attcgcgatg cagccgctcg atcaccgggg caggcgtgcc tttcggcgcg agcatgccca
    10681 tccagacata gagctcatag tccttcaagc cctgctcgat catggtcggg atctccggcg
    10741 cggccgaagc gcgcctcgcc ccggtgacgg cgagcgcccg cgcccggccc tcgcggatgt
    10801 ggccgcgcat cgcggcaagc ccgggcatcg ccgccgacac ctgcccaccg agcaggtcgg
    10861 tcaccgcctg cgcgctgccc ttgtacggga tgtgattcag ctggatgccg gccatggtgg
    10921 cgaacatcgc catgaataga tgctgcgcgc tgccgttgcc cgaggatgaa tagtcgacct
    10981 taccgggccg ctccttggcg taagcgatga actcgcgcag cgtcttcgcg ggcaccgagg
    11041 gattcacgag cagcaccgcc tgctcgctcg cgaggagcga gatcggggcg aaatcctcca
    11101 ccgggtcgtg ggtcagcctg gagtacagcg aggcgctgat ggcgttggga ttggacgcga
    11161 ggagcagcgt gtagccgtca gccggggcct tcaccacttc ggcgcttccg agcatcgcgc
    11221 cctgccccgg cttgttctcc accaccatct gctggccgag ctgcttcgac atctcctgcg
    11281 cgatcacgcg catgggccca tctgaagaac cgccggcgga atacggcacg accacccgga
    11341 tgggacgcga cggatattcc tgggcgcacg cggcgccggc ggcaaggcag gcaaagacaa
    11401 ggatggctct tttcactgga tgctctctcc gttggaatag caatagtctg caatgtcgcg
    11461 cggccggcaa taccagacgg ccgcgcggcc ggcgcagtgc cgtagcgcgc gccgaagctg
    11521 gcgcacccgg aacggctggc cgaatacgta aggatgcacc gacacgttca tgaccagcgg
    11581 gtgggcctcg cactgccgcg ccatctcgtc gaactggtcg accaccgcgt cggcgaaggt
    11641 cgcggcggac cacctgcgat ggatcacacc ctgcgcatcg tcggtctcga ccgggtacgg
    11701 gatcgagagg atccgccccg cccgggtccg catccagatt ggctggtcgt ccatcggcca
    11761 atccatcacg tagcgatagc cgagctcctt gagcagatcc ggggtgtgcg ccgtttcgta
    11821 agcccccgag cccatccagc ccgacggcgg cgcgccctcg tgccgcgcga tcgtgtccgt
    11881 gacctcgcga aggatgcgct cctcgtcggt ctgccatagc ccgcgcaggt tctcagcgtt
    11941 ggtgcggccg tgcgcgatca cctcgtcgcc gcgccggcgg atcgcgccgg tgatctgcgg
    12001 cgcgtattcg tagatcaggc tgttggtgca atgcccggcg ggaaggccga gctcgtccag
    12061 caggtcgaac aggcgccaga tgccgatgcg attgccgtag tcccgccagg cgtagttgcg
    12121 gtgcgtctgc ggctcgccgg gcttggccgg atcgttcccc aaccctgcgc cgaacgcgaa
    12181 ccactcgatg ttcgtggtaa gcaggaacgc caggcgcttt ccgcccggcc aactgtagtc
    12241 ccgacgttca accaacggaa cgaaatcgta gcggccgtgc tccggtaacg gcctcaatcg
    12301 actttcgccc ccgacgcctt caccaccggc gctagcttcg caatctcggc ggcagtctgc
    12361 cgctcgaact cctcgggcga ggtcgcgatc gggtcggcgc cgatgttgcg ccacaccggc
    12421 tgcatctccg gcgcctgcac cgctcttgcg aactcgccgt tgatccggcg caccagcgcg
    12481 cgctccatgc ctttcggcgc gaggaccccg gagtagagca cgatttcgaa gttcggcagc
    12541 cccgactcga tcatggtcgg aacctcgggc gcggcgccgc ttcgcttcgg cgtggtgacc
    12601 gcgagcgccc ggagcttgcc gcccctgacg ttcgagatcg cgggcggcat ggtcgagaac
    12661 acgaaagcca cctcgccgcc gaggatcgcc gcggtcgccg gattgcttcc cttgtaaggg
    12721 acatgcagcg cgtcgacgcc ggccgaggac ttcagcattt cggaggagag atgcacgatg
    12781 gtgccgttgc ccgaagaccc gtaggagtac ttgcccgggt tcgccttcag cagggcgatc
    12841 agctcgcgca cgttgttcgc aggcgtcgac ggatgcacga ccagcatgag cggggtggag
    12901 gccaccatcg cgacgaagct gaagtccttc atcgagtcgt acggcagctt cgcgtacagg
    12961 ttcgcgttga tcgcgtgcgt ggtgatgtcc tgcagccaca gcgtgtagcc gtcgggcgcc
    13021 gatttgacgg tgaaatcccc cgcgatggtc gtgcccgccc cgggcttgtt ctccacgatc
    13081 acctgctgcc cgagctgctt ggagagcgga atggccaccg cgcggcccag cacatcggag
    13141 atgccgccgg cggcgaagcc caccaccagc cgcaccggct tggtcggata ttcggactgc
    13201 gccagcgcgg ttcccccgaa aaggagaacc aggcacatcg ctcttctcat gaaagtcctt
    13261 tcatggcgcg ccatagcgcc tcgacttcgc tcttttccac gccgttggtg atgaacacca
    13321 gcgccggctc gccgccctcg acgggtctca ggcggtcgaa cacatggcgc acgccctgga
    13381 ccaggaccag cccgctttcc accggaacga tacccttcac gcggagcaaa tcggcgccgc
    13441 gcagatcgac cagcgtcgag aggaagcgct gcagcagccc gcgccgcacc ggctgctcga
    13501 acctgagcgc aaagctgcga atcctcgagt cgtgcgccgc cgccgcgaaa acgggggccg
    13561 tccccggttt cgcttcgagg atgactcgag gatcggcctc gccgtagctc gcccgaatgc
    13621 attgcgccgc ggggtttagc gctcgaattt ctgcttctgc cactgtagga tcgccgacgc
    13681 ggtccacttt tgaaagaacc acgacatccg ccgcggcgat ctgcgcgcgg gcgatctcgg
    13741 cagagacttt tctggattca agatcgaagg agtcgaccac cgtgacgatc ggtccgagct
    13801 tgaagtcgcg cgcgagcgcc tggtcggcgt agagcgtctg cgccaccggg ccggggtcgg
    13861 cgaggccgct ggtttccacg atcacgcggt cgaacccggc gatgtcgccg cgccgccggc
    13921 gcatcgcggc gccgatcagc gtctcctcga gatcgccgcg caggtggcag cacaggcagc
    13981 cgttcggaag cgccaccgct ttttccgtgg tcccttcgac caggacgtgg tcgacgggaa
    14041 tctcgccgaa ctcgttcacg atcaccaggg aatcggagaa ccggacgagc ttgttgacaa
    14101 gcgtcgtctt gccgctgccg agaaacccgg tgaccaccac gaccggggtc ctgccgtccg
    14161 gcgtcacacg ttcatttcga ggcagtacag tcccccgccg ccgcggtcca ccgtgaacac
    14221 gatgccgcgg tcgtcgacga acacgtcgtt cagctgcacc gcgccggtct tggcgcgttc
    14281 cggggtcggc ggcacgaagt acgcgacctc gcgcggcgaa tagggatcgg tcacgtcgta
    14341 ggcgcgcacg ccgccgttga agaaggtgcc gagcacgatg tcctcggagc gccatgcccc
    14401 gggcaccggc ggattttcgt gcaggttgtg cgcaccgaag cgcccgccgc ggctcttgaa
    14461 cgcatccacc ggcggcatcg gcagcgtggc gatgctcacg aggtggcgct catcgcgcgc
    14521 gtcgagcagc cacaccagct tcggccagtc gtcggcgttg tccttgaccg actcgtcgct
    14581 caccaccagc aggccgcggc cgaagaacgg cagcgcggta tggttgaagc cgttgtacgg
    14641 cggcgaattg gtccagtgcg acagcagctt cggccgcgcc cggtcgctga tatcgagcac
    14701 catgatgccg ccgtcgatgt aggcgaggta gcagcggtcg ggccgctcgg gatagacgtt
    14761 ggtgttgtgg gcgcggaagc cggcgtcgaa cttcgcgggc aggcgctgcg gcggcggcgc
    14821 gtcgtcgctc tccatggtcc cgggcaggtg ccagcggccg accgccaccg gcttcgacgg
    14881 gttggccacg tcgacgatgc ggtagatctg gtcgtccttc gggttgcgcg gcttgagctc
    14941 cgggtcggcg caagccatgt gcaccgtgct tccgtcgacg aaccagagct ggtggcagcc
    15001 gcgcgaatgc gggccggagg cgtcgaagaa cgagatggac ttcggctttt ccggaacgct
    15061 gatgtcgaac agctcgaagc cggcgggttt caggccgaac ttggtcacct ggtaggccac
    15121 cgccatcagg tcgcccgcca cctcgaggga gttggagcgc atctgcgcgt gcggcagatc
    15181 ggtttgcacg atcaccttgg gcgcgcgggg atcgctgacg tcgaccgcgg tgaagttctt
    15241 gggcgcgctc tcgtgcgcga gccacagtac gcggcggccg tcgcgcgcaa gctgcatcga
    15301 ggtgccttcg cccatgccgc cgaagccatt gagggtgtgc tgcgcgagca gcttcatgtt
    15361 caggaacatg cgcctaagat atccgagaaa ccggcgccgc ggacctcgtg aagagccatg
    15421 tttcgagcgc cgtgacctcc agcggccgcg agaccagcgt gccctggatc tggtcgcagc
    15481 ccagcgcgcg cagcaggtcg gccgcctcct gctcctcgac gccctcggcc accacgatca
    15541 ggccgaggtt gtgcgcgagg tcgatggtcg agagcacgat cgcccggtcg cgcatgtcgg
    15601 tcagcatgcc gcgcaggaag gagcggtcga tcttcagctc gtcgacgctg agctgcttca
    15661 cgtagctgag tgacgagtag ccggtaccga agtcgtcgat cgcgcgcttc acgccgagct
    15721 tctccaggcg cttcagcacc tcgatcgcgc gcgccgcgtc ctgcatgacc ccgctttcgg
    15781 tgatctccag gcacaggagc tcgggcggca ggcggtggcg ctcgagcgcc gcggtgagca
    15841 gcccgctgag ctcggggttg agcaggtcct gcgccgagat gttgaccgag atcggcgtcg
    15901 gccgcccggc catgcgccag gccgcgccct gcctgaccgc ggtgtcgatc acccaggcgg
    15961 tgatcgtctt gatgaagccg gtctgctcgg cgaacgggat gaagcggtcg ggcgtgatca
    16021 agcctttcgc cgggtgggcc cagcgcacca gggcctccac gccgctcagg atgccgctcg
    16081 ccaggtcgat cttgggctgg tagaccaggc gcaactggcc ccggtcgatc gcctggcgca
    16141 gctccgagag cagcgagagc tgctcctcgc gctgctcctg gacgtgcgca tcgtagaccg
    16201 cgaatcccgt gttgccgcgc ttggccgagt acatcgcggc atcggcgcgg cgcatcaggg
    16261 tgtcggcgtc gccggcgtgc gccgggaagg ccgcgatgcc gacgctcgcg cgcacgtcga
    16321 gcgagtgctg cccgatcgcg atcggcgcct cgagcgcggc gacgaccttc tgcgccacgc
    16381 cgcgcgcctc gtcgacgccg ctgttcacca gcaggacggc gaactcgtcg ccgcccagcc
    16441 gcgcggtggt gtcggacttg cgcaccacgc gcgtgatgcg cagcgccacc tgctgcagca
    16501 ccatgtcgcc gacgtggtgc ccgagcgtgt cgttgatgta cttgaagcgg tccaggtcca
    16561 tcacgagcac cgtgagcgcc gcgccgccgc gcttggcgcc ctcgagcgcg acccgcagcc
    16621 ggtcgttgaa caggacccgg ttgggcaggt ccgacagggg atcgcggtag gcgagcttga
    16681 ggatctgctc ctcgcgcgcg gcgatcgcct cgcgcatcag gttgaagcgg cgcgccagct
    16741 cgctcacctc gtccgttccc tcggcctcga tcgcctgcgc gtagtcgccc tcctcgatgc
    16801 gcttggtcat ggccgagaac agcgagagcg gccgcgtcac gctgcgcgcg atcagcacgc
    16861 tgccgacgat cgacgccacg atcgagcccg ccaccagcag cacgagaagc gggccgagcc
    16921 ggcggtacgg cgcctcgtcg agagagcgcg cgaggacggc gacgatgcgc gagtcgcttc
    16981 catcgagcgc catgacgaac gactcgtatt cccggccgcc caggtccagc ggcatcgcgc
    17041 ggtcgccggc ggcgttgccc ttcggcagcg tgcgcagcac cgcctcctgc agctcggaat
    17101 cggcggagga gccgagcacg cgccagacct gcccctcgcg gctcacgaac gtgacctcca
    17161 gggcggcgag attcgcgagg tcgcgcgcca ggcggtcgtc caccgtgaag ccgaccaccg
    17221 tccaggcgat cggcgtcggc gcgcgcaccg gcaccaccgc aacttggaac gcggcgccgt
    17281 cgaccagcac catcccggcc gctgagccgt cgcgctcggc ggcctcgagc agccgcgtga
    17341 acgggaacgg ctgaccggcg cgccgccggt cgaaggtgtc ggcgatgagc tgccgctcga
    17401 gcgacaccag cgccatcgtg tcggcgcgga tgcgcgagcc gtggttgacc agcaccgact
    17461 cgatggtcga cacgtcgcgc gtcgccgcgg cctcgcggaa cgcgaaatcg agcgacacga
    17521 tcagcgcggc ctgcgtcagg cgggcatggt tctgctccag caggcgccgg aagatcttct
    17581 cgcccacgac gatctcgtgg cgcacgttgt cgcgcgcatt gcgctcgttg gcggcattga
    17641 ccagcagcag cgtgatgccc tgcaccacca cgagcagcgc gacgaagaac agcacgatgc
    17701 ggctttgcag gctgcgcagg cgcatggccg ccccgcccct tagtagcgag cgccgcggcg
    17761 cgagaccggc gcgcgccgca cccgcgtctc gggcttgagc gcgactaccc attcgagggc
    17821 ggcgttgccc tcggcgccga gctccgccgg ccgccgcgtg gtttcctcgg tggcggcgag
    17881 tcggggatgc cagacgtgca ccgtgtagcg tccggcaggc aggcccgcga tacgcgcggt
    17941 tccctgggcg ccggtggtgg cgaaatgcgg cgacggcgaa acgtagaggt agccgaccat
    18001 ccagtcgtgg atgttgcagc cgatggcgac cacgccgggc tggtcgaaca ccacccgctg
    18061 cgccggcgtg ccgacgtaca gcggcagctc gaagcgcttc gccggcgaga acgaatacac
    18121 gtgatggcgc acgtcgtcgc ggttcggaaa cgacaccgcg gtcccggtct ggatcgcctt
    18181 cacgtgcggc acgaactcga ggtcgatctg ctcgacctgc tcgacgcgcg agcgcccttc
    18241 ctgcggcgcg gcgtcttgcg gaaccgcgac caccacggcg tcgggcaccg ccctgccctg
    18301 gtcgtcgcgc accgtcgcgc gcagatccgc ggcatgcgcg agcggcgcga gcgccaggac
    18361 cgcggcgagc attcccggtc ctgtgccgag cctagtcacg gtagacgagc gacacgctgt
    18421 acagattcgc gtcgtagtcc agcccgccgc gcgcgctggt gcgctcgctc gtaagcgccg
    18481 cgtcgaccga cgcatttacg ccgagcgccc acgaaaggcc gagggtgtag gagtcggtgc
    18541 gcgccccggt cagcttgtac gcgatgtagt cggggccgaa agccgggtcg gctgcgatcg
    18601 ccgcgctctc gcggaagatc tgctggttgc gccgagtgct cgaggcgaca tctccgtgcc
    18661 gcgccgcgag ggcgccgaag gcgagcagct tctcgccgaa cgcgtagctg cccctgacgg
    18721 acaggctcct cccctgaagc gaaaacggct tgcccgagat gcctgggacc gttccgaagt
    18781 cttcgctctg ccagcgccgg tcgtaggcgg cgccgagcga gacttcgaac gacgggccca
    18841 atcgcttgcc gagctcgagc gcgagcgccg cgcggttgcc gtcgcgcacc ggctcggagt
    18901 aatcctcgcc gaagaacgcc gcgtcggcgg cgagccacgg cgcggtgagc ccgaggccga
    18961 gcttgcggcg ccaggccgct cccgcgccga gggccgcgtg atcgaggccc gggaaatcgt
    19021 cgtagagcgc cccgcgcgct tctgctcgca ggacgaggtc gccgccggcc acgacgagct
    19081 gccgcccgag tgcgccacgc aacgcgagcg cgtgatcgcg cacgatgtcg ctctcgcgct
    19141 gcgcccgcga caggttgtcg tcgtaggtgc gcgcgaggtc ggcgtcgagc gtccagtgcg
    19201 ccgccgcagc cgccggcagc gccgcggcga caagcgcgag cgcggttcgc atcctggcat
    19261 tgtgagccgc gccgccgcgg aggacaaagc ggtaagctcc tcctgacatg aaattgccca
    19321 ttgccgccac tctggccggc gtgctttttt cactgtttgc gctctcggcc ggcgcccagg
    19381 gctggccggc caagccggtg cgcatcgtcg tcgcctaccc gcccggggga ggcatcgacg
    19441 tcctggggcg gcagctcgcc gacaagctcg cgcccctctg gggccagccg gtggtggtgg
    19501 aaaacaagcc tggcgcgaac accatcgtcg ccaccgacgc agtcgcgaaa tcagccgcgg
    19561 acggccacac catcctgctc accacggatg cgactttctc gatcaatccg cacctctacg
    19621 cgaagcttcc ttacgacgcg cagcgcgatt tcgcgcccgt gaccatgctg gtcctgctgc
    19681 agcagctcct cgtcgcgcat ccgtccttgc ctgcgaacag cgttgccgag cttgttgcgc
    19741 tggcgaaggc aaagcccggc gcgatcaact acgcctccta tggcagcggc agccagccgc
    19801 acctcgcagg ggagatgctg aagaacaagg cgggcatcga cttggtgcat gtgccgtaca
    19861 agggcatctc gctcgccgtg ccggcggtga tggcgggaga agtgcagctc accttcgccg
    19921 gcatcgccac gtcgatgccg cagctcaagg ccgggcgcat taaggcgctc gcgatcggcg
    19981 gcgcggcgcg ctcgccgctc ctgccgcagg tgccgacctt caccgagctc ggctacccgg
    20041 aagtggagac gcacgcctgg ttcggcctct tcgtgccggc cggatcgccg cgggaagcgg
    20101 tggcgcgaat ccagcaggac acaaagagga tcctcgacga gccggacttc cggcaaaaac
    20161 agctcgtcga caaaggctat gaagtcgttg gcagcgcctc gcaagacttt gtcatgtatt
    20221 tgagaacgga cagcgaaagc cgcggccgcg cggtcagaat ctcgggagca aaggctgaat
    20281 agaaaaacac cttggaaacg gtcttcgtcg agcctgcgcc acggctcgca gtctccgtcg
    20341 ccggcgaggg cgagctggtg cttttcctgc acggcatccg cggcaaccgc cgcaactggg
    20401 ccgcgcagat cgagtttttc tcccgccgct tcagggcggc cgcgtgggac gcgcgcggct
    20461 acggcgactc cgacgactac gaaggcgcgc tgcagttcga atacttgacc ggcgacgtga
    20521 tgcgcgtcgc ggagcacttc aaggcgcgca agctccacct ggtgggactc tccatgggcg
    20581 ggcgcatcgc gcgcaacgtc gcgctgcgcg cgcccgagcg gctgcattcg ctggtcctga
    20641 tcagcaccaa tccgggcttc gatgctatga gcccggaaag cgtgaagcga ttcgtcaacg
    20701 agcggcgcac gcgcacgccg caaacgctgg cgaagctgct cggcagccgc gccagccgcg
    20761 cggcgtacga cgagcttctc gacagcgtcg cgcgcatcca cgacgcttcg taccagaaga
    20821 ccctggaggc ctcggtggcg caggaccgcg cggcgccgat cgagcagatc cgcgtgccga
    20881 ccctggtgat cgcgggcgag gaagacaccg tgtatcccgc ggagctcgcc gagcagatgg
    20941 cgcggcgcat ccccggcgcc gagctgctga tgctcgagcg caccggccac ctcgccaacc
    21001 tcgagcagcc ggaccggttc aacaaggcgg ttttcgactt cgtgtcgcgg cacagcgcgc
    21061 gatgacataa catcgcctct tccaggacag gaggagacga tggcttcagc gaagagagtc
    21121 gcgctcgtca ccggcgcggg cagcggcatc ggcaaatcgg ccgcgctggc gctgctcaag
    21181 gacggctacc acgtcgcgct ggtcggcagg cgcaaggaca tgctcgagaa ggccgccgcc
    21241 gagtcgggcg cgaaggatcg cgcgctggtg ctggcggcgg acattacgaa agatgagcag
    21301 gtgaaggatg tctttgcaaa aaccaaatca gcctggggcc gcctggacgt cctgttcaac
    21361 aacgccggca tcggcgcgcc ggcggtgccg atggaagacc tgccgatcga gaaattccgc
    21421 gaggtggtga acatcaacct ggtcgccatg ttcatgtgca cccaggaagc gatccgcatc
    21481 atgaaggcgc aggaccccaa gggcggccgc atcatcaaca acggctcgat ctcggcgcat
    21541 gcgccgcggc cgatgtcggt cgcctacacc tccaccaagc acgcggtcac cggcctcacc
    21601 aagtgcatct cgctcgacgg ccgcaagaac gacatcaccg cgagccagat cgacatcggc
    21661 aacgcgctca ccgagctcgc cgcgcgcatg gcgaacggcg tgccgcaggc cgacggctcg
    21721 atcaagcccg agccgctgat ggacgttaag cacgtcggtc aggcggtgct gcatatggcc
    21781 aacctgccgc tcgacgtgaa cgtgcagttc atgaccatca tggcgacgaa gatgccgttc
    21841 gtggggcggg gttaggtcac cgccaggtag gctttttcca gcgaaccatc ggcggcaatc
    21901 tctgccgcgc tgccgctcgc cacgatgcgc ccgccctcga tgacataggc gcggtcggca
    21961 agcgcgagcg cgagaccggc catctggtcg accagcagga tggtggcaga ttcggcgcgc
    22021 aggcggtcga gcgcggcgaa gaggtcgttg gccacctgcg gcgcgagccc gagcgatggc
    22081 tcgtcgagga gcaggaggcg cggcttggcc atcaggccgc gcccgatcgc gagcatctgc
    22141 tgctcaccgc ccgagagcag cccggcgcgc tgctgcagcc gctcgcgcag gcgcggaaag
    22201 cgctcgagga cggcgtcgat ttcggcgcgg tcgactttct tccgggagaa ggcgccgagc
    22261 agcaggttgt cgagcaccga aagctcagcg aacacctgcc gtccctcggg caccagcacg
    22321 acgccgcgcg caacgacgcg gtgcgccggc tgggtcgcga gctcctcgcc ggcgagcgcg
    22381 atcccgccgg cgaccggccg gtgcaggccc gagagcgcgc gcatcagcgt cgacttgccg
    22441 gcgccgttgg cgccgagcac cgcgaccatc tcgccctggc gcacgccgag gtcgatgcct
    22501 ttcagcaccg gctccgcgcc gtagcccgcg gcgaggcggt cgacctcgag cagcggcgcg
    22561 cccggttctg cacgcttcgc gaccaggcgt tgctgctcct cgccgaggta agccttgcgc
    22621 accgcgggat cgcgctgcac ttccgccggc gtaccgctcg caatgcgcag cccggcatcg
    22681 agcaccacca cgcggtcgga gattcccatc accaccgaca tgtcatgctc gaccagcacc
    22741 accgcgatgc cggcgtcggc gatgcgccgc aggagcccgg cgagcttcgc cttgtcgtgc
    22801 accgacagtc ccgccgccgg ctcgtcgagc agcagcacgg cagggcgcgt ggcgagggcg
    22861 cgcgcgatct cgaccaggcg cttgtcgacg tgggccaggt ccgcggcgcg ggcgtccatg
    22921 tcgccgccgt agccgacgaa ggccgcgagg ctctcgtcac cggaggcgat cgccaggttc
    22981 tccgccacgc tcatcgagcc gaagagttgc gtggtctggt aggtgcgcgc cacgccgctg
    23041 cgcgcgatgc gccacgccgg ccggccttcc agcgcctcgc cgccgatgcg gatgctcccc
    23101 gcggcaggcc ggtagaaccc gctcagcatg ttgagcaccg tggtcttgcc ggcgccgttc
    23161 ggcccgatca ggctggtgac ctctcccggc tgcgcctcga acgcgatgtc gtgcgccgcg
    23221 cgcacgccgc cgaaggcaat ccccaggccg cgcaccgcca gcgcggcttt cgccctcgga
    23281 gcgatcgggg ccgccgcggc ggcggcgggc cggggcgcgc gtcttttcga aacaaggcgc
    23341 agcacggtgc cgaccacgcc ttcgggggcg agccacagga cgacgagcag caacgcgccg
    23401 aagaagagca gccggtactc ggcgagcgca gcgaggaact cgggcagcag cacgacgatc
    23461 gccgcgccga ccagcggacc cgcgacgtag cccgcgccgc cgatcatcac caccagcacg
    23521 aacaggatcg actgcaggaa tgcgaacgaa ctgggcgtga cgaagccgga cagcggcgcg
    23581 aacagcccgc ccgcaagacc ggccagcgcc gccgaacccg cgaacgcgac ggtcttgacc
    23641 gtcagcggat cgatgccgac cgattccgcg gcgacgtcgc tgtcgcgcac cgcgcgcatc
    23701 gcggcgcccc acgcgccgag cgacagccag cggaacacca gcagcgcgca cgcggcggtg
    23761 acgatcgcta ccaccgccac cgtccgctcg ctgcccgcga agccgaagag cgccgggcgt
    23821 ggcaggttca tgagcccgtt ctggccgccg
//
LOCUS       DATBSZ010000189        18722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1261480, whole genome shotgun sequence.
ACCESSION   DATBSZ010000189 DATBSZ010000000
VERSION     DATBSZ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18722
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261480"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1736
                     /locus_tag="VIG70_12095"
     CDS             <1..1736
                     /locus_tag="VIG70_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8251338.1"
                     /translation="DRVVANPPGMEAQLRALHKQALAGEPVVVETVRVRKDSNKRDVE
                     LRGVPIQHRGQPHVLYIGRDISERKRAEASLRASEEQYRAIFNAAADALVLRDAEARV
                     VDVNPAFLEISGYTREEVISGSRWIFAGPDQAALAVEMHRRVIAGESVQFEVNGRHKD
                     GSRFVVEMRAVPIRYRGQPHALGMARDITQRKALESRLRQAQRMEALGHLTGGVAHDF
                     NNLLAAIMGYIVLASERDTAARDAKLSGYLDKALASSRQARDLIQQMLTFSRGQRASP
                     RPLALADAVAESLKLLRSSLPSSLEIETLLEKTPAVMLDPVQLDQVLLNLSINARDAM
                     AGLGRIEVAVRTTALREKAVCASCRKRFRGEFAELSVADSGPGIAPPVLERMFEPFFT
                     TKEVGRGSGMGLATVHGIVHEHKGHVLVESAPGAGSRFRILLPMLPPETSAATTVAAA
                     PAKPSRKRLSGRVLVVDDEKAVGEFMRELLESWGLQASAMSSPAGVLERIAREPPDLV
                     ILDQTMPGITGMNIAREIAAARPGLPVVLYTGNSDRVEKEELDAAGVRALLQKPVEPD
                     ALYELLRTHLR"
     gene            1760..2629
                     /locus_tag="VIG70_12100"
     CDS             1760..2629
                     /locus_tag="VIG70_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8251339.1"
                     /translation="MKALLAWYFVAVWGGGYVFTKAGLQYAPPFTFLTLRFCFGLLCL
                     APIVLIWRPVWPRTPAGFAHLAMAGLLMHVVQLGGSHYAQYLGMSAGVAALVISCQPL
                     ITAVVAEKLLGERVHRGQWLGVFVGLAGVALVVGHQLEIREVTFGSLLGTLIALAGVT
                     AATLYQKHFSPRADLKAAAAIQFAASAAVLAPLAVAVENFQVTWAPQLLVSIAALVIF
                     ASLLGVSVLYYLLRHGEATRVTSMMYLPPVFAVVLEAAWFGVVPSALALAGIAVTCAG
                     VAMTVWRPRGRSS"
     gene            2629..3768
                     /locus_tag="VIG70_12105"
     CDS             2629..3768
                     /locus_tag="VIG70_12105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8251340.1"
                     /translation="MEIVYFVALNALGHLAFVGCRMTTTLFALELGASEFTVGVLMSL
                     FALLPTFLSVSAGRLIDRTGPLRPLEVSMGTLALAAALPFAFPSLVTLYFSTSLLGVS
                     FMYVHIAMNSVFGAHGSPEQRAVNFSWLALGFSISNSIGPLVAGYAIDGLGHAKAFAL
                     LAVFPAIALALLWVRKAPLPRPEHVPLAKRSGVLDLLRIPGLRQTFWVSLLLATGWDL
                     YTFIVPLYGARIGLSAAAIGVILSTFALATFTVRLAMPVLIKQLRQWVIIFAALAISG
                     SCYLLFPFAQSVPPLLLLSFVLGLGLGSAQPVIMSLLYAASPPGRQGEAVGLRTSLLN
                     GSHTLIPLASGAASTAVGMAPVFWLLAAGLLGGAWFARRQVKSSP"
     gene            3756..4769
                     /locus_tag="VIG70_12110"
     CDS             3756..4769
                     /locus_tag="VIG70_12110"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEY8251341.1"
                     /translation="MFPMRRVTLSQFLVGQQREKQLINADLRLLIETIARACKAISTR
                     VNKGALADGLGNAGTENVQGEAQKKLDVLSNEILLEANEWGGNLAALVSEEMEDPRPI
                     PTHYPRGEYLLLFDPLDGSSNIDVNISVGTIFSVLRCPKLADGKYCDPNEQAFLQPGR
                     KQVAAGFAVYGPTTVFVLTVGDGAYGFTLDRETYTFVQTHPAIRIPEDTQEFAINASN
                     ARRWEPPVKRYIDECLAGKDGPRGKDFNMRWVASMVADVFRVLSRGGIFMYPRDAKNK
                     DGRLRLMYEANPMAFIVEQAGGAATDGRTPILDLQPKGLHQRCAVVLGSRNEVERVAA
                     YHK"
     gene            complement(4815..5066)
                     /locus_tag="VIG70_12115"
                     /pseudo
     CDS             complement(4815..5066)
                     /locus_tag="VIG70_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
     gene            5299..5931
                     /gene="pdxH"
                     /locus_tag="VIG70_12120"
     CDS             5299..5931
                     /gene="pdxH"
                     /locus_tag="VIG70_12120"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY8251342.1"
                     /translation="MNIADLRQEYMRSGLSETQADPDPIRQFERWFDDALRAQLPLAN
                     AMTLATVTPSGAPSARIVLLKGVDRGGFLFYTNYLSRKGRELERRAAACLLLLWTQLE
                     RQVRIDGTVQKVGAGESDAYYATRPLGARLSAWASAQSETVASRKQLETAMERSRERY
                     RDNPPRPPHWGGYRVDPQEIEFWQGRSDRLHDRLRYTRAGGSWTIERLSP"
     gene            5996..6880
                     /locus_tag="VIG70_12125"
     CDS             5996..6880
                     /locus_tag="VIG70_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8251343.1"
                     /translation="MLSGGVSSEIWKVGDVCVKRALPRLRVAQRWEAPIERNRYERLW
                     LEAAAAAAPGAAPRVLACDDKAGLFAMEYLHSPVWKERLRQGNADPRFAAQVGTTLAA
                     IHAATAGRPEVAQRFATDEIFYAIRLEPYLVATGAAHPDLREILTSLVRRTASTKVCL
                     VHGDVSPKNILVGPRGPVFLDAECAWYGDPAFDLAFCLNHLLLKCLWVPASRSAFLAC
                     FDALASSYLQGVSFPGIEQRTASLLPALLLARVDGKSPVEYLDEQSCHLVRVIAKELL
                     PAASTLAQVRRAWQRSST"
     gene            6859..8103
                     /locus_tag="VIG70_12130"
     CDS             6859..8103
                     /locus_tag="VIG70_12130"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY8251344.1"
                     /translation="MAKIIDVTARQVWDSRGHPTVEAEVIVGRGTTPLASGRAIAPAG
                     ASTGSGEAKNVDVRTAVHNVRTRIRDGLRALSVSDQAKLDQRLIEIDGTPDKSRLGAN
                     AMVAVSLACAHADAASQKIPLWKKFSGEKRVSLPVPQIQIFGGGAHARGKCDVQDYMI
                     ICIGAGSFAEALEWTSQVYAAAGKRLAKRGALQGVADEGGYWPAFKTNEEGLAELVGA
                     IADAGLEPGADVAIALDIAATQLYRGGSYHLALEGRALSAEQLHAMWMRWIERYPIVS
                     IEDPFAETDNAAMRAFTKATSIQVVGDDFFVTDAKRVKAAEGACNTVLLKPNQVGTVT
                     ETLACWDAARAAGYRAIVSARSGETEDVSIVHLAVGWGVPQLKVGSFSRSERMAKWNE
                     ALRIEEALGAKALPFPARKLFK"
     gene            8286..9740
                     /gene="kaiC"
                     /locus_tag="VIG70_12135"
     CDS             8286..9740
                     /gene="kaiC"
                     /locus_tag="VIG70_12135"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEY8251345.1"
                     /translation="MKTERAEPARALPKTPTGIEGLDEITAGGLPKGRPTLVCGGPGC
                     GKTLLATEFLVRGAMQFGEPGAFISFEETGEELAQNVRSLGFDLGALVADNKLAIDHV
                     HVERAEIEETGEWDLDGLFVRLAYAIDSVGAKRIVLDTIESLFGGFTNEATLRAELRR
                     LFRWLKDKGVTAVITGERGERSYTRQGLEEYVSDCVILLDHRVTEQVSTRRIRIVKYR
                     GSIHGTNEYPFLIDETGFSVLPLSGLQLDHDVSDERISSGVPGLDAMLDGKGYFRGSS
                     ILVSGTAGTGKTSVAAHFAQEVARRGERCLYLAFEESPQQLIRNMQSIGVNLEPFVKK
                     GLLTIRSSRPSLYGLEMHLVQIHKMIAKFDPTAVVIDPISNFVNGSTTGEAYSMLLRL
                     VDYLKVKRITALFTHLTSGGRPTEATENGISSVIDTWLLLRDAEIGNERTRGLFVLKS
                     RGMPHSNQIREFLLTPKGIELKRVIGKAGKGDTR"
     gene            9737..10024
                     /locus_tag="VIG70_12140"
     CDS             9737..10024
                     /locus_tag="VIG70_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEY8251346.1"
                     /translation="MNQPEKWELRLYTAGQSPKSLAALANLKRVCDDHLAGRYSIEVI
                     DLLKNPRLAKDDEIIAIPTLVRKLPQPLRKLVGDLSDTERTLVGLQLKPRS"
     gene            10042..10734
                     /locus_tag="VIG70_12145"
     CDS             10042..10734
                     /locus_tag="VIG70_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8251347.1"
                     /translation="MEQNLTQSPSFEQELQQLRERLQEAEEMHRAISHGEVDAFVVGP
                     DANTRVLLLANAYQRYRQLVERMNQGAITATPSGQILFANQRFSDMLGVPLAQMYTAP
                     FESYVGVGDRARLSRFLMLSARDSRVEVELSRRDGTSIPVRFSIASFADGYATVLATD
                     MRPMQWPSLAIDALDSIRSSLEKLNHTLAGNGAARDALSNISEEINGLARMIDQMLDV
                     EAMGGPRSKGNP"
     gene            10821..11384
                     /locus_tag="VIG70_12150"
     CDS             10821..11384
                     /locus_tag="VIG70_12150"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY8251348.1"
                     /translation="MATGVSRAVIEVLLDRVHQGAATLSPEGILAYANQRLASMLGRS
                     RAELVGKAFADLVAEHDRETFANALAAGRDGASQCRLAMPRAKAANVPNGGPNGGGEL
                     QALVTFAPLGHGQMSCLLTDLTQGQHVGVLAHEMRNVLGTMRNSVELLRRSALDADGQ
                     RALESIERQSGRILALMEDLRRVNPKE"
     gene            11489..12646
                     /locus_tag="VIG70_12155"
     CDS             11489..12646
                     /locus_tag="VIG70_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8251349.1"
                     /translation="MRLAQFITENIEDILVEWEAFAASLSPGQTMTSLALRDHARQIL
                     QAVAADIETNQTELEQAYKSQGFVTIAEATRTAAMTHGALRYLAGFDLRQLAAEFRAL
                     RASVLRLWLKQGVHDHSAFYEMTRFNESIDQALAESIANYSDEVALSRDTFLAILGHD
                     LRSPLSAIANSGLYLGSPGLLPAGAPLDAVGRITRSAAKMGSMIRDLLEYTRTRLGRA
                     IPISPQAVNMEHICSLALDEIRGAHPERIFRLEISGNLDGQFDSERLQQVLSNLLSNA
                     VRHGDRKQPITLSARGAPDRITLQVRNYGRPIPPDQLQVIFNPLVQIPSALVDDDGGP
                     STSLGLGLYIAHEIVAMHGGTIVAESSDTHGTVFSARLPRAPGQARREARP"
     gene            complement(12756..13403)
                     /locus_tag="VIG70_12160"
     CDS             complement(12756..13403)
                     /locus_tag="VIG70_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251350.1"
                     /translation="MLKLSGVTKRFGARSVLTGISLEVAAGEYIAIIGDSGIGKSTLL
                     NVIAGLEPVDAGEVLFEEKSLTAMDDDALTLLRRDHFGFVFQAFHVLPQLNVEQNVAL
                     PLLLRGITSTEKAKAAIASVGLAGREASTPRELSGGELQRVAIARALVGEPRLVLADE
                     PTGNLDPENARQVLDLFRIQVKTRGAAAILATHSEAAAAGCDRRYRLTAQGLSLM"
     gene            complement(13405..14796)
                     /locus_tag="VIG70_12165"
     CDS             complement(13405..14796)
                     /locus_tag="VIG70_12165"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8251351.1"
                     /translation="MKRALAGLLSLPALALAQSLAITGVTVVDATGAAPRPDMTVLVS
                     ADRISALGKTGEIGVPADSRVVEGKGKFLIPGLWDMHVHWNDGAYLPLFIANGVTGIR
                     IMWGLPPHLEQRKRIAEGSLLGPRMVIAGPLIDGPRPYWKESVAAGTPEQGRQAVRRT
                     RAEGYDFVKVYDSLPRAVFFAVVDEARKLNIPVSGHTPAAVGVAAASDAGQKSIEHLT
                     GMLVGVSSDEEVLRREFAAVSRLQPAAETAAMLRGLGARMLDTYDAGKARALYAKLKT
                     NGTWQVPTFTVLRFNSYAREPQHAADPRLRYMPSWVREMWASDPRFRELLPEDLEHQK
                     RIFQRRLELVGEMHRAGVGILAGSDVLNPWCFAGFSLHDELEWLVKAGLTPMAALQAA
                     TRSPAMYLERAQDLGTVEKGKIADLVLLDADPTLDIRNTRRIAAVVAGGRLFDRAALD
                     RMLGDVERLAGAN"
     gene            complement(14793..16280)
                     /locus_tag="VIG70_12170"
     CDS             complement(14793..16280)
                     /locus_tag="VIG70_12170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEY8251352.1"
                     /translation="MKRSIVDYISEHARRSPDRVAIHYKEKQFSYGELEEAAAHCRGA
                     LVARGIRPGDRVSLVMSDSPEMVIAFLGIMGMGAIAVPCSTLLPPEGLGYVFKDSEAK
                     LVIVSPEHLENAKKGGAPRTVLMNELMDGARPAELGTFERDTPCLVLYTSGSTGQPKG
                     AVHRHGHMPWTVESVARRVYDLQPDDRLFSVPRLFFAYGLGNSLSIPLGTGASTILVS
                     ERPTPALVAEVFAKYRPTVFFAVPTVFRMILEHVRQGNRIDTGSLKFSVSAGEVLPVA
                     TWNEWKALTGTEIIETIGTTELLHAFIHNYRDRNRPGSSGVALEGYEVKLVDESGAVV
                     QGAGRGHLHARGGSAIPYYLNKPEKTAETIRDGWVRTGDVYRRDEEGFYWFEGRSDDL
                     FKCSGLWVSPGEVEDAVCRHPAVLEAAVIAATDAHGGTIPAAYVMLRPGHSPGEALES
                     AIKEEAAKTLPRYKRPQRIHFMEQLPRTPTGKVQRFKLRQMAAAK"
     gene            16267..17619
                     /locus_tag="VIG70_12175"
     CDS             16267..17619
                     /locus_tag="VIG70_12175"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251353.1"
                     /translation="MLRFMGKNLREQYEKAQQLGKPAFLERPYFGSPAAPAQAETNAS
                     QIWGHINAGYLIAYEYTRWWKESLALRNAAVIGDWSWLNKTLVRGRDAARLMNYATVK
                     DLFRQQVGQVMYTPMVNAEGKVAIEGLTLKLAEDEYLFTQSAGLKWLEFLREKTGLKV
                     SLEDVTPDYTCYALQGPRSTEVLEAVTGEAWRDLRFSRFRRTRLFETETLVARQGVTG
                     EIGYEFLMRTDTGRAHELWRKLREVGREFGLRELGFKAQMIGHTETGIATVIRDFLPA
                     RGTPEQLRRMMRHWMSVEELECIDYPIEEHFCSPAELGWGHTIDLDKDDFFGKAALTS
                     RAPRRRLMGLEWSSEDMAELHAAQYGDAPSAPPPDLPTGQFRVFFLKVLEGGWASGYA
                     YSPTLRRMISLARLDRSILPGTKVQVQWGGFCDEPKISIRAKVVELPFLARKREAVIP
                     "
     gene            17674..>18722
                     /locus_tag="VIG70_12180"
     CDS             17674..>18722
                     /locus_tag="VIG70_12180"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251354.1"
                     /translation="MRKPILAALAAALAFAMPAQAQQKIKIGFISTFSGPQGVMGQYM
                     KESVELALDHLGRKVGGLEVEMHYGDDQVKPDVGVQVAEEMLKKHQVDFVSGIIWSNV
                     MMAVMPVVTGAGKIMVGSNAGASPLAGSQCNELYFSTSWNNDQTPEAMGKFLQDQGVN
                     DIYVMAPNYQAGKDMVAGLKRYYKGRIVEETYTKLGQQDYQAEITQLRSKNPKAVFVF
                     YPGGMGIQFLRQYDQAGLHGKLPLYSVYTVDEISIPAVKDAALGVKEARFWSPDLKNA
                     ASEKYVADYKKKYGKLPVFYGAQSYDAIMLIDSAVRGTKGNLKDTQALVKEMRKANFN
                     SVRGKFEFNTNHHPI"
BASE COUNT         3160 a         6406 c         6104 g         3052 t
ORIGIN      
        1 aggaccgcgt ggtcgccaat ccgccgggaa tggaggcgca gctgcgcgcc ctgcacaagc
       61 aggcgctggc gggcgaaccc gtggtcgtcg agaccgtgcg cgtgcgcaag gacagcaaca
      121 agcgcgacgt ggagctgcgc ggcgtgccga tccagcaccg cggccagccg cacgtgctgt
      181 acatcggccg cgacatcagc gagcgcaagc gcgccgaagc cagcctgcgc gcgagcgagg
      241 agcagtaccg cgcgatcttc aacgccgccg ccgacgcgct ggtgctgcgc gacgccgagg
      301 cgcgcgtggt ggacgtcaac ccggcgttcc tcgagatcag cggctacacg cgcgaagagg
      361 tgatcagcgg cagccgctgg atcttcgccg ggcccgacca ggcagcgctg gccgtcgaga
      421 tgcaccggcg cgtgatcgcc ggcgagtcgg tgcagttcga ggtcaatggg cggcacaagg
      481 acggctcgcg cttcgtggtc gagatgcgcg cggtgccgat ccgctaccgc ggccagccgc
      541 acgcgctcgg catggcgcgc gacatcaccc agaggaaggc gctggagtcg cgcctgcgcc
      601 aggcgcagcg catggaggcg ctcggccacc tgaccggcgg cgtggcgcac gacttcaaca
      661 acctgctcgc cgcgatcatg ggctacatcg tgctcgcctc ggagcgcgac actgccgcgc
      721 gcgacgccaa gctctccggc tacctggaca aggcgctcgc gtcctcgcgc caggcgaggg
      781 acctcatcca gcagatgctg accttcagcc gcggccagcg cgcctcgccg cgcccgctcg
      841 cgctcgccga cgccgtggcc gagtcgttga agctgctgcg ctcctcgctt ccttcgtcgc
      901 tggaaatcga aacccttctc gaaaaaaccc cggccgtgat gctcgacccg gtgcagctcg
      961 accaggtgct gctgaacctc tcgatcaacg cgcgcgacgc catggccggc ctcgggcgca
     1021 tcgaggtcgc ggtgcgcacc accgcgctgc gcgagaaggc ggtgtgcgcg agctgccgca
     1081 agcgcttccg gggagagttc gccgagctgt cggtcgcgga ctcgggtccc ggaatcgcgc
     1141 cgcccgtact ggaacggatg ttcgagccct tcttcaccac caaggaggtg gggcgcggca
     1201 gcggcatggg gctcgccacg gtgcacggca tcgtgcacga gcacaaaggc catgtcctgg
     1261 tcgagagcgc gcccggcgcc ggcagccgct tccgcatcct cctgccgatg ctcccgcccg
     1321 aaaccagcgc ggccaccacc gtcgccgcgg ccccggcaaa gccttcccgc aagcgcctga
     1381 gcgggcgcgt actggtggtc gacgacgaga aggcggtggg cgagttcatg cgcgagctgc
     1441 tcgagagctg ggggctgcag gcgagcgcga tgtcgagccc cgccggggtg ctcgagcgca
     1501 tcgcacgcga gcctccggac ctggtgatcc tcgaccagac catgcccggc atcaccggga
     1561 tgaacatcgc gcgcgagatc gccgccgcgc gccccggcct gcccgtggtc ctctacaccg
     1621 gcaacagcga ccgggtggaa aaggaagagc tcgatgcggc cggcgtgcgc gcgctgctgc
     1681 agaagccggt cgagcccgac gcgctttatg aactgctgag gacccacctg cgctagggtc
     1741 ctagaatcgg cggcttcgca tgaaagcgtt gctcgcctgg tacttcgtcg ccgtctgggg
     1801 cggcggctac gtcttcacca aggccgggct gcagtacgcg ccgccgttca ctttcctcac
     1861 gctgcgcttc tgcttcggcc tgctgtgcct cgcgccgatc gtcctcatct ggcggccggt
     1921 gtggccccgg accccggccg gattcgcgca cctcgccatg gccggcctgc tcatgcacgt
     1981 ggtgcagctc ggcggcagcc actatgcgca gtacctcggc atgtcggccg gggtggcggc
     2041 gctcgtcatc tcctgccagc cgctgatcac agccgtcgtt gcagagaaac ttctaggtga
     2101 aagagtgcat cgcggtcagt ggctcggggt cttcgtcggc ctggccggcg tggcgctcgt
     2161 ggtcgggcac cagctcgaga tccgcgaggt gaccttcggc agcctgctcg gcacgctcat
     2221 cgcgctcgcg ggggtgacgg ccgccacgct gtatcaaaag catttctctc caagggcgga
     2281 cctcaaggcc gcggcagcga tccagttcgc tgcttccgcc gcggtgctgg cgcctctcgc
     2341 agttgcagtg gaaaactttc aagtgacgtg ggcgccgcag ctgcttgttt cgattgcggc
     2401 cctcgtgatc tttgcgtcgc tgctcggggt tagcgtgctc tactacctgc tgcgccatgg
     2461 cgaagccacc cgggtgacga gcatgatgta ccttccgccg gtgttcgccg tggtgctcga
     2521 ggctgcgtgg ttcggcgtgg tgccgagcgc gctcgcgctc gccggcatcg ccgtgacctg
     2581 cgcgggcgtc gcgatgaccg tctggagacc ccgcgggaga tcgtcgtagt ggaaatcgtc
     2641 tatttcgtcg ccctgaacgc gctcgggcac ctcgccttcg tcggctgccg gatgacgacg
     2701 accctgttcg cgctcgagct cggcgcctcc gagttcaccg tcggggtgct gatgtcgctg
     2761 ttcgccctgc tgccgacgtt cctctcggtc agcgccgggc gcctcatcga tcgcaccggg
     2821 ccgctgcgcc cgctcgaggt ctcgatggga acgctcgccc tggctgcggc gctgcctttc
     2881 gcctttccgt ccctggtcac cctctatttc tccaccagcc tgctcggcgt ctctttcatg
     2941 tacgtgcaca tcgccatgaa cagcgtgttc ggcgcccacg gctcgcccga gcagcgcgcg
     3001 gtgaacttct cgtggctggc gctcggcttc tcgatctcga actcgatcgg gccgctggta
     3061 gccgggtacg cgatcgacgg gctcggccat gcgaaggcgt tcgcgctgct agccgtgttt
     3121 ccggccatcg ccctcgcgct tctgtgggtg cgcaaggcgc cgctgccgcg gcccgagcac
     3181 gtgccgctcg ccaagcgctc gggcgtgctc gacctgctgc gcatccccgg gctgcgccag
     3241 accttctggg tgagcctgct cctcgccacc ggctgggacc tctatacctt catcgtcccg
     3301 ctgtacggcg cgcgcatcgg cctctccgcc gccgcgatcg gcgtcattct ctcgacgttc
     3361 gcgctggcga cctttaccgt gcggctcgcg atgccggttt taataaaaca acttagacaa
     3421 tgggtgatca tcttcgcggc gctcgccatc tccggaagct gctacctgct gttcccgttc
     3481 gcccagagcg tgccgccgct gctcctgctg tcgttcgtgc tcggcctcgg gctcggcagc
     3541 gcgcagccgg tcatcatgtc cctcctctac gcagcgtcgc cgccggggcg ccagggcgag
     3601 gcggtcggcc tgcgcacttc gctcctgaac ggcagccaca ccctgatccc gcttgcttcc
     3661 ggcgcggcct cgaccgccgt gggcatggcg cccgtgttct ggctgctcgc cgccggcctg
     3721 ctgggcgggg cgtggttcgc aagaagacaa gtaaaatctt ccccatgaga agagtcacgc
     3781 tgagccagtt cctcgtcgga cagcagcgcg agaagcagct catcaacgcc gacctcaggc
     3841 tcctcatcga gaccatcgcc cgcgcctgca aggcgatcag tacgcgggtg aacaagggcg
     3901 ccttggccga cggcctcggc aacgccggca cggagaatgt ccagggagaa gcccagaaga
     3961 agctcgacgt gctctctaac gagatcctgc tcgaggcgaa cgaatggggc ggaaacctcg
     4021 ccgcgctggt ctccgaggag atggaggatc cgcggcccat ccccacgcac tacccgcgcg
     4081 gggagtacct gctgctgttc gacccgctcg acggctcgtc gaacatcgac gtgaacatct
     4141 ccgtgggcac gatcttctcg gtgctgcgct gcccgaagct cgccgacggc aagtactgcg
     4201 acccgaacga gcaggccttc ctgcagcccg ggcgcaagca ggtcgccgcc ggcttcgcgg
     4261 tgtacggccc gaccacggtg ttcgtgctga cggtcggcga cggcgcctac ggcttcaccc
     4321 tcgatcgcga gacctacacc ttcgtgcaga cgcaccccgc gatccgcatt cccgaggata
     4381 cgcaggaatt cgcgattaac gcctccaacg cgcggcgctg ggagccgccg gtgaagcgct
     4441 acatcgacga atgcctcgcc ggaaaggacg gcccccgggg caaggacttc aacatgcgct
     4501 gggtcgcctc gatggtggcc gacgtgttcc gcgtgctctc gcgcggcggc atcttcatgt
     4561 acccgcgcga cgcgaagaac aaggacggac gcctgcgcct gatgtacgag gccaacccca
     4621 tggccttcat cgtcgagcag gcgggcggcg cggcgaccga cggccgcacc ccgatcctcg
     4681 acctgcagcc gaaggggctg caccagcgct gcgcggtggt gctcggctcg agaaacgagg
     4741 tcgagcgggt cgccgcgtac cacaaataaa aaggcccctt gcggggcctc ttcattcttt
     4801 cctcgaaagc tgcgttactt gagatgccgg ctgaccagct tggtcatctc gaacatcgac
     4861 acctgaccct tgccggcgaa caccgccttg agcttgtcgt cagcattgat catgcgccgg
     4921 ttcttcttgt cctgaaggcc gttacgcttg atgtagcccc agatcttctt ggtcacctcg
     4981 gtgcgcggca tcgcgctcga gccaaccacc gacgcgagcg ggccgctcgg ggtcatcggt
     5041 ttcatgaaag cggcattcgg cttacgcttc ttgccggctt tcttcgcttt cttcttcgcc
     5101 atcgatttct cctctaacga actggtctag aacgggatga aagggttttg agcttcggtc
     5161 cgggttcccc cgccacaggg cagcggtgac gtgaagatgc cgaatataca ttcgcgtgtc
     5221 aacgcttttc agagggagat tgcgacactt tccctcggca gtcggatttc agccacgctc
     5281 cggactagaa tcgcgcgcat gaacatcgcc gacctgcgcc aggaatacat gcgctccggg
     5341 ctctcggaaa cgcaggccga tcccgatccg atccgccagt tcgagcgctg gttcgacgat
     5401 gcgctgcgcg ctcagctgcc gctcgccaac gcgatgacgc tcgccaccgt cacgccctcg
     5461 ggcgcacctt cggcgcgcat cgtcctcctg aaaggcgtcg atcgcggcgg ttttctcttt
     5521 tataccaact acctcagccg caaaggacgc gagctcgagc ggcgcgcagc cgcctgcctg
     5581 ctcctcctct ggacgcagct cgagcggcag gtgcgcatcg acggaaccgt gcagaaggtc
     5641 ggcgccgggg agtccgacgc ctactatgcg acgcgccccc tcggtgcgcg gctctcggcc
     5701 tgggcctcgg cgcagagcga aaccgtggct tcccgaaaac agctcgaaac cgccatggag
     5761 cgctcccgag agcgttaccg cgacaaccct cctcgccctc cccattgggg cggataccgc
     5821 gtcgatccgc aggaaatcga gttctggcag ggccgcagcg accggctgca cgaccggctg
     5881 cgctatacgc gcgcaggcgg ctcctggacg attgagcgcc tctctccctg aagaggttct
     5941 cgggctgctg agaggcgcgg cgctcgtttc acgaaatgaa acgcccgcct tcgaggtgct
     6001 gtccggcggc gtgtcctccg aaatctggaa agtcggcgac gtgtgcgtca agcgcgcatt
     6061 gccccgcctg cgcgtcgccc agcgctggga agcgccgatc gagcgcaacc gctatgagcg
     6121 cctgtggctc gaggccgcgg ccgctgctgc gcccggcgcc gcgccgcgcg tccttgcctg
     6181 cgacgacaag gccgggctgt tcgcgatgga atatcttcat tcgccggtgt ggaaggagcg
     6241 actgcgccag ggcaacgccg atccgcgctt cgcagcgcaa gtcggcacta ccctcgccgc
     6301 gattcacgcc gccactgcgg gaaggcccga agtcgcgcag cgtttcgcga ccgacgagat
     6361 cttctatgcg atccgcctcg agccttacct ggttgccacc ggagcggccc accccgacct
     6421 gcgcgagatt ctgacgtctc tcgtcaggcg aacggcgtcg accaaggtct gcctggtgca
     6481 cggcgacgtg agcccgaaga acatcctcgt cgggccgcgc ggccctgtgt ttctggacgc
     6541 cgaatgcgcc tggtacggcg accctgcgtt cgatctggcg ttctgcctga accacctgct
     6601 gctgaagtgc ctctgggtgc cggcaagccg gtccgccttc ctcgcatgct tcgatgccct
     6661 ggcatcgagc tacctgcagg gcgtgtcctt tccgggcatc gagcaacgca ccgcttcgct
     6721 cctacccgcg ctgctgctcg cccgggtaga cggcaaatcg cccgtggaat acctcgatga
     6781 gcaaagttgc catctggtaa gagtgatagc aaaagagctg ttgcctgccg cctcaaccct
     6841 cgcccaggtc cgccgcgcat ggcaaagatc atcgacgtaa cggcccggca ggtctgggat
     6901 tcgcgcggcc atcccacggt cgaggcggag gtcatcgtag ggcgcggcac cacgccgctc
     6961 gcctcggggc gcgcgatcgc ccccgccggc gcctcgaccg gctccgggga ggcgaagaac
     7021 gtcgacgtgc gcacggcagt gcataacgtc cgcacgcgca tccgcgacgg cctgcgcgcc
     7081 ctcagcgtgt ccgaccaggc gaagctcgac cagcgcctga tcgagatcga cggcacgccc
     7141 gacaagagcc ggctcggcgc caacgccatg gtcgccgttt cgctcgcctg cgcgcatgcc
     7201 gacgcggcct cgcaaaagat ccccctgtgg aagaaattct ccggggagaa acgggtatcg
     7261 ctgccggtgc cgcagatcca gatcttcggc ggcggcgcgc acgcgcgcgg caagtgcgac
     7321 gtgcaggact acatgatcat atgcatcggc gcgggcagct tcgccgaggc gctggagtgg
     7381 acctcgcagg tctacgccgc ggccggaaag cgcctggcga agcgcggcgc gctccaaggc
     7441 gtcgccgacg aaggcggcta ctggccggcc ttcaagacga acgaggaagg cctcgccgag
     7501 ctggtgggcg cgatcgcgga cgccggcctc gagccggggg ccgacgtggc gatcgccctc
     7561 gacatcgccg ccacccagct ctaccgcggc ggcagctacc acctcgcgct cgagggccgg
     7621 gcgctctccg ccgagcagct gcacgcgatg tggatgcgct ggatcgagcg ctatcccatc
     7681 gtctcgatcg aagatccttt tgccgagacg gacaacgctg cgatgcgcgc ctttaccaag
     7741 gcgacctcta tccaggtcgt cggggacgat ttcttcgtca ccgacgcgaa acgagtaaaa
     7801 gccgcggaag gcgcctgcaa taccgtgctg ctgaaaccga accaggtcgg caccgtgacc
     7861 gagaccctcg cctgctggga cgctgcgcgc gcagccggct accgcgcgat cgtctcggcc
     7921 cgctcgggtg agaccgagga tgtctcgatc gtccacctgg cggtcggctg gggcgtgccg
     7981 cagctcaagg tcggcagctt ctcgcgctcc gagcgcatgg caaaatggaa cgaggcgctg
     8041 cgcatcgagg aagccctcgg cgcgaaggcc cttcccttcc ccgcccgcaa gctcttcaaa
     8101 tgacccaccc gaaaaacagg gacagaccac gtttttcttg cgccctggaa ggaaagaaag
     8161 cgtggtccgt ccctgatttg ccgtgaaggg tggggcggct ggaggcgggg caaagccgtc
     8221 atatgtcatc tacatgctgc taatatcggg accccccgga gcgaccgaaa aatatctccc
     8281 agacaatgaa gaccgagcgc gctgagcccg ctcgtgcgct gcccaaaacg cccaccggga
     8341 tcgaaggcct cgacgagatc accgccggcg ggctgcctaa agggcgcccg acgctcgtgt
     8401 gcggcgggcc gggctgcggc aagacgctgc tcgccaccga gttcctggtg cgcggcgcaa
     8461 tgcagttcgg cgagcccggc gcgttcatca gcttcgaaga gaccggcgag gagctcgcgc
     8521 agaacgtgcg ttcgctcggg ttcgacctgg gcgccctcgt cgccgacaac aaactcgcga
     8581 tcgaccatgt gcacgtggag cgcgccgaga tcgaggagac cggcgagtgg gatctcgacg
     8641 gccttttcgt gcgcctcgcc tacgcgatcg actcggtggg cgcgaaacgc atcgtgctcg
     8701 acacgatcga gtcgctgttc ggcggcttta ccaacgaggc gacgctgcgt gcggagctgc
     8761 ggcgcctgtt ccgctggctg aaggacaagg gcgtgaccgc ggtgatcacc ggcgagcgcg
     8821 gcgaacgctc ctatacgcgc caggggttgg aggaatacgt ctccgactgc gtgatcctgc
     8881 tcgaccatcg agtgaccgag caggtctcca cgcgccgcat tcgcatcgtg aagtaccgcg
     8941 gctcgatcca cggcaccaac gagtatccgt tcctcatcga cgagaccggg ttctcggtcc
     9001 tgcccctgag cggcctgcag ctcgaccacg acgtcagcga cgagcgcatc tcgagcggcg
     9061 ttccgggcct cgacgccatg ctcgacggca agggctactt ccgcggctcg tcgatcctgg
     9121 tgtcgggtac cgccggcacg ggcaagacca gcgtcgcggc gcatttcgcc caggaggtcg
     9181 cgcggcgcgg cgagcgctgc ctgtacctcg ccttcgagga atcgccgcag cagctcatcc
     9241 gcaacatgca gagcatcggc gtgaacctgg agccgttcgt gaagaagggc ttgctgacga
     9301 tccgttcgtc gcgccccagc ctgtacggcc tcgagatgca cctggtgcag atccacaaga
     9361 tgatcgcaaa gttcgatccg actgcggtgg tgatcgaccc gatcagcaac ttcgtcaacg
     9421 gctcgacgac gggcgaagcc tactcgatgc tgctgcgcct ggtcgactac ctgaaggtga
     9481 agcggatcac tgcgctcttc acccacctca cgagcggtgg ccggcccacg gaggccaccg
     9541 agaacggcat ctcgtcggtg atcgacacct ggttgctgtt gcgcgacgcc gagatcggca
     9601 acgagcgcac ccgcggcttg ttcgtgctca agtcgcgcgg catgccgcac tcgaaccaga
     9661 tccgcgagtt cctgctaacc cccaagggaa tcgagctcaa gcgagtcatc ggcaaggccg
     9721 ggaaaggcga caccaggtga atcaacccga gaaatgggag cttcggctct ataccgcggg
     9781 ccagagcccg aagtcgctgg cggcgctggc gaacctgaag cgcgtgtgcg acgaccacct
     9841 ggccggacgc tattcgatcg aggtgatcga cctcctcaag aacccgcgcc tcgccaagga
     9901 cgacgagatc atcgccatcc cgacgctggt gcgcaagctg ccccagccgc tgcgaaagct
     9961 ggtgggcgac ctttccgaca ccgagcgcac gctggtcggc ctgcagctca agccgcggtc
    10021 ctgaagcgag ggacatccga catggaacag aacctgacgc aatcgccgtc cttcgagcaa
    10081 gagctccagc aactgcgtga acgcctgcag gaagccgagg aaatgcaccg cgccatcagc
    10141 cacggcgaag tcgacgcctt cgtggtcggt ccggacgcca atacccgggt gctgctgctc
    10201 gcgaacgctt accagcgcta ccggcagctg gtggagcgga tgaaccaggg cgcgatcacg
    10261 gccacgccca gcggccagat cctgttcgcc aaccagcgct tcagcgacat gctgggcgtg
    10321 ccgctcgccc agatgtacac cgccccgttc gagagttacg tcggcgtagg cgatcgcgcc
    10381 cggctgagcc gcttcctgat gctcagcgcg cgcgactcgc gcgtcgaggt cgagctgagc
    10441 cggcgcgacg gcacgagcat cccggtgcgg ttctcgatcg cctcgttcgc cgacggctac
    10501 gccaccgtcc tcgcgacgga catgcgcccg atgcagtggc cgtcgctggc gatcgatgcc
    10561 ctcgactcca tccgcagctc gctcgaaaag ctcaaccaca ctctcgccgg caacggcgcg
    10621 gcgcgcgacg cgctgagcaa catcagcgag gaaatcaatg gcttggctcg gatgatcgac
    10681 cagatgctgg acgtcgaggc gatgggcggg ccgcgcagca agggaaatcc ctagtcaggg
    10741 aaatcccgac tccaaagcgt gtagtacgcg tcatgaaagc tggatagacg gcttcgctat
    10801 agcgattaca gactctcgcg atggcgaccg gggtctcgcg agcggtgatc gaggttctgc
    10861 tcgaccgcgt ccatcagggc gcggcgacgc tctcgcccga gggaatcctc gcctacgcca
    10921 accagcgcct cgcgtcgatg ctcggtcgga gccgcgcgga gctcgtcgga aaggccttcg
    10981 ccgatctcgt cgccgagcac gatcgcgaga ccttcgcgaa tgcgctggcc gcggggcgcg
    11041 acggcgcgtc gcaatgccgt ctcgcgatgc cgcgcgccaa ggcagccaac gtccccaatg
    11101 gcggccccaa cggcggcggc gagctgcagg cgctcgtgac cttcgcgccg ctcgggcacg
    11161 ggcagatgag ttgcctcctc accgacctca cccagggcca gcacgtcggc gtgctggcgc
    11221 acgaaatgcg caacgtgctc ggcacgatgc gcaactcggt cgagctgctc aggcgctccg
    11281 cgctcgacgc cgacggccag cgcgcgctcg agagcatcga gcgccagagc ggccgcatcc
    11341 tcgcgctgat ggaggacctg cggcgcgtca acccgaagga atagtcgcaa cctccgcata
    11401 agttcggcac cgcacggacc ctcatgcgtc cgaccgctaa agtacgacgg ggcgtgatgt
    11461 tgacgccgcg cgaacacgga ggcaatgcat gcggctggct caattcatca ccgagaacat
    11521 cgaagacatc ctggtcgagt gggaggcatt cgccgcatcg ctttcgccgg ggcagaccat
    11581 gacgtcgttg gcgctgcgcg accacgcgag gcagatcctg caggccgtgg cggcggatat
    11641 cgagaccaac cagaccgagc tcgagcaggc ctacaagtcg caaggcttcg tgaccatcgc
    11701 ggaggccacc cggacggccg cgatgacgca cggcgccctg cgctaccttg ccggcttcga
    11761 cctgcgccag ctcgccgcgg agttccgggc gctgcgggcg agcgtgctgc gcctctggct
    11821 gaagcagggc gtccacgacc acagcgcgtt ctacgaaatg acgcgcttca atgagtcgat
    11881 cgaccaggcg ctggccgaat cgatcgcgaa ctactccgat gaggtggcgc tctcgcgaga
    11941 cacgttcctt gcgattctgg ggcacgacct gcgaagcccg ctctccgcga tcgccaactc
    12001 cggcctctac ctcgggtctc cgggcctgct gcccgccggc gcgccgctcg acgccgtcgg
    12061 ccgcatcact cgcagcgccg cgaagatggg ctcgatgatc agggatctgc ttgagtacac
    12121 gaggacgcgg ctgggccgtg cgatcccgat atcgccgcaa gcggtgaaca tggagcacat
    12181 ctgcagcctc gcgctcgacg aaatacgagg cgcgcacccg gaaaggattt tccggctcga
    12241 gatttcaggc aaccttgatg ggcagtttga cagcgagcgc ctgcaacagg tgctctcgaa
    12301 tctgctcagc aacgcggtcc ggcacggcga caggaaacag ccgatcaccc tttccgcgcg
    12361 cggcgccccg gacaggatca cgctgcaggt caggaactat ggccgcccga tcccaccaga
    12421 ccagctgcag gtgatattca acccgctggt gcaaattccg tcagcactgg tcgacgacga
    12481 cggcggtcca tccaccagcc tgggtctcgg gctttacatc gcccacgaaa tcgtggcgat
    12541 gcacggcggc actatcgtgg ccgagtcctc ggacacgcac ggcacggtat tcagcgcgcg
    12601 gctgccgcga gccccgggcc aagcccgccg cgaagcccgc ccctagcggg cctcccactc
    12661 tcaagcgcgg gcgatcgcct ccgcgcccga gctgacaaac accaggtcgt agtccgtctc
    12721 caggacagca gccatcgcga gaagaatcgt acctattaca ttaggctcaa tccttgcgcg
    12781 gtgagccggt agcgccggtc gcatcccgcg gcggcggcct ccgaatgcgt cgcgaggatg
    12841 gcggccgcgc cccttgtctt gacctggatc ctgaagagat ccaggacttg gcgcgcgttc
    12901 tccggatcca gattgccggt cggctcgtcg gccagcacga gcctcggctc gccgaccagc
    12961 gcccgcgcga tcgccacgcg ctgcagctcg ccgcccgaaa gctcgcgcgg cgtgctggct
    13021 tcacgcccgg ccagccccac ggacgcaata gctgcttttg ccttttcagt tgaggtaatt
    13081 ccccgcaaga gcagcggcag cgcgacgttc tgctccacat tgagctgcgg caggacgtgg
    13141 aacgcctgga acacgaagcc gaagtggtcg cggcgcagga gcgtcagcgc gtcgtcgtcc
    13201 atcgcagtca ggctcttttc ttcgaaaagc acttcccccg catcgaccgg ctcgagtccc
    13261 gcgatcacgt tgagcagcgt ggacttgccg atgccggagt ctccgatgat tgcgatgtac
    13321 tcgccggcag cgacctccag ggagatgccg gtcaggacgc ttcgcgcgcc gaagcgcttc
    13381 gtcaccccgg aaagcttcag cacgtcagtt ggcgccggcc aggcgctcga cgtcgccgag
    13441 catccggtcg agcgccgcgc ggtcgaagag ccttccgcct gcgacgacgg ccgcgatccg
    13501 gcgcgtgttg cggatgtcca gggtcgggtc ggcgtcgagc agcaccaggt cggcgatctt
    13561 ccccttctcc acggtgccga ggtcctgcgc gcgctccagg tacatcgcag ggctgcgcgt
    13621 cgccgcctgc agcgcagcca tcggggtcag gcccgccttc accaaccatt ccagctcgtc
    13681 gtgcaggctg aagccggcaa aacaccatgg gttgagcacg tcggatcccg cgaggatgcc
    13741 cacgccagcg cggtgcatct cgccgacgag ctcgagccgt cgctggaaga tccgcttctg
    13801 gtgctcgaga tcctcaggta gcagttcccg gaaacgcggg tcgctggccc acatctctcg
    13861 cacccatgac ggcatgtagc gcagccgcgg atcggcagcg tgctgaggct cgcgcgcgta
    13921 ggaattgaag cgcagcaccg tgaaggtcgg cacctgccat gtgccgttcg tcttgagctt
    13981 cgcgtagagc gcgcgcgcct tgcccgcgtc gtaggtatcg agcatgcggg cccccaggcc
    14041 tcgcagcatc gcagcggtct ccgccgcggg ctggagccgc gacacagcgg cgaactcgcg
    14101 gcgcaggacc tcctcgtccg acgagacccc gaccagcatc ccggtcaggt gctcgatcga
    14161 cttctggccg gcgtcggaag cggccgcgac gccgaccgcc gccggcgtgt ggccggaaac
    14221 cgggatgttc agctttctgg cctcgtcgac caccgcgaag aacaccgcgc gtggcagcga
    14281 gtcgtagacc ttgacgaagt cgtagccctc cgcgcgcgtg cgccgcaccg cctgccggcc
    14341 ctgctcgggc gtccccgcgg cgaccgactc cttccagtaa ggcctcggcc cgtcgatcag
    14401 cggacccgcg atcaccatcc tcggcccgag caggctgccc tctgcgatgc gcttgcgctg
    14461 ctcgaggtgc ggcggcagcc cccacatgat gcggatgccg gtcacgccgt tcgcgatgaa
    14521 gagcggcagg taggcgccgt cgttccagtg gacgtgcatg tcccacagcc cggggatgag
    14581 gaactttcct ttgccctcca cgacgcggga atcggctgga acgccgatct cccctgtctt
    14641 tcccagggcg gagatgcgat cggcgctgac gagcaccgtc atgtcgggcc gcggcgccgc
    14701 gccggtggcg tcgacgacgg tgacgcccgt aatggctagc gactgtgcga gcgcaagtgc
    14761 gggcagtgag agcaggccgg caagagcacg cttcacttgg cggcggccat ctgccgcagt
    14821 ttgaagcgct gcaccttgcc cgtcggcgtg cgcggcaact gctccatgaa gtgaatgcgc
    14881 tgcggccgct tgtaacgcgg cagcgttttc gccgcttctt ccttgatcgc gctttcgaga
    14941 gcctctcccg gcgaatggcc gggccgcagc atcacgtacg ccgcggggat ggtgccgccg
    15001 tgcgcatcgg tcgcggcgat caccgccgcc tcgagcaccg ccgggtgccg gcacaccgcg
    15061 tcctcgacct cgccgggtga gacccagagc ccggagcact tgaagaggtc gtcgctacgg
    15121 ccttcgaacc agtagaagcc ttcctcgtcg cgccggtaga cgtcgccggt gcgcacccag
    15181 ccgtcgcgga tcgtctcggc ggtcttctcg ggcttgttca ggtaatacgg aatcgcgctg
    15241 ccgccgcggg cgtggagatg ccctcgcccg gcgccctgca ccacggcgcc gctctcgtcg
    15301 acgagcttga cctcgtagcc ctcgagcgcg acgcccgagc tccccgggcg gttgcggtcg
    15361 cggtagttgt ggatgaaggc gtgcagcagc tcggtcgtgc cgatggtctc gatgatctcg
    15421 gtgccggtga gcgctttcca ctcgttccag gtcgcgaccg gaagcacttc gcccgcggag
    15481 accgagaact tgagcgagcc tgtatcgatc cggttcccct ggcgcacgtg ctccaggatc
    15541 atgcggaaca cggtcggcac ggcgaagaac accgtgggcc ggtacttggc gaacacctcg
    15601 gcgacgagcg cgggcgtcgg ccgctcgctg acgagaatgg tcgaagcgcc ggtgccgagc
    15661 gggatcgaca ggctgtttcc caggccgtac gcgaagaaca ggcgcggtac ggagaacagg
    15721 cgatcgtcgg gctgcaggtc gtagaccctg cgcgccacgc tctccacggt ccacggcata
    15781 tggccatggc ggtgcaccgc gcccttcggc tggccggtgc tgcccgaggt gtagaggacc
    15841 aggcagggcg tgtcgcgctc gaacgtccca agctccgccg gtcttgctcc atccatcaat
    15901 tcattcatca ggacggtgcg cggcgccccg cccttcttcg cattttccag atgctcggga
    15961 gaaacaatga ccagcttcgc ttcgctatcc ttgaagacgt agccaaggcc ctccggcggc
    16021 agcagcgtgc tgcacggcac ggcgatcgcg cccatgccca tgatgccgag gaacgcgatc
    16081 accatttcgg gcgaatcgct catcaccagg gaaacgcggt cgcccggccg gattccgcgc
    16141 gcgacaagcg ccccacggca atgcgcggcg gcttcctcga gctcgccgta gctgaactgc
    16201 ttttccttgt agtgaatcgc cacgcgatcc gggctgcggc gggcgtgttc cgagatgtaa
    16261 tcgactatgc tgcgtttcat ggggaaaaac ctgcgtgaac agtacgagaa agcccagcag
    16321 cttggcaagc cagccttcct cgagcggccc tacttcggtt cgcccgccgc gccggcgcag
    16381 gccgagacca atgcgagcca gatctggggg cacatcaacg cggggtattt gatcgcctac
    16441 gaatacacgc gctggtggaa ggaatcgctg gcgctgcgca atgccgcggt gatcggcgac
    16501 tggagctggc tgaacaagac cctggtccgg ggacgcgatg ctgcgcggct catgaactac
    16561 gccacggtca aggacctgtt caggcagcag gtcggccagg tcatgtacac gccgatggtg
    16621 aacgcagaag gaaaagtcgc aatcgagggc ctgaccctca agctcgccga ggacgaatat
    16681 ctgttcacgc agtcggccgg gctcaagtgg ctcgagttcc tgcgcgagaa aaccggcctg
    16741 aaggtctcgc tcgaggacgt gacgccggac tacacctgct acgcgctgca gggaccgcgc
    16801 tcgaccgagg ttctcgaggc cgtcaccggg gaggcgtgga gagatttgag gttttcgcgt
    16861 tttcgcagga cgaggctctt tgagacggaa accctggttg cacgccaggg cgtcacggga
    16921 gaaatcggct acgagttcct gatgcgcacc gataccggac gtgcgcacga gctgtggcgc
    16981 aagctgcgcg aagtcggccg cgagttcggc ctgcgcgagc tcggcttcaa ggcgcagatg
    17041 atcggccaca ccgagaccgg catcgccacg gtgatccgcg acttcctgcc ggcgcgcggc
    17101 acgccggagc agctgcgccg catgatgcgc cactggatgt cggtcgaaga gctcgaatgc
    17161 atcgactatc cgatcgagga gcacttctgc tcgccggcgg agctgggttg gggccatacg
    17221 atcgacctcg acaaggacga tttttttgga aaagcggcat tgaccagccg ggcgccgcgg
    17281 cgccggctca tgggcctcga atggtcgagc gaggacatgg ccgagctcca tgcggcgcag
    17341 tacggggatg ccccctcggc gccgccgccc gatctgccga ccggccagtt tcgcgtcttc
    17401 ttcctgaaag tcctcgaggg cggctgggcc tcgggctatg cctacagccc gacgctgcgc
    17461 cgcatgattt ccctggcacg cctggatcgg agcatcctgc ccggcactaa agtgcaggtg
    17521 caatggggcg ggttctgcga cgagccgaag atcagcatcc gggcgaaggt cgtcgagctt
    17581 ccgttccttg cccgaaaacg agaggcggtc atcccctagg cgcatcttgc gttatccttg
    17641 catcctgcta ggactttcct gaggaggagg aacgtgcgca aacccattct cgcggcgctc
    17701 gccgcggctc tcgctttcgc catgccggcg caggcgcaac aaaaaatcaa gatcggcttc
    17761 atcagcacct tctccggccc gcaaggcgtg atgggccagt acatgaagga atcggtcgag
    17821 ctcgcgctcg accacctggg gcgcaaggtc ggcggcctcg aggtcgagat gcactacggc
    17881 gacgaccagg tgaagcccga cgtcggcgtg caggtcgccg aggagatgct gaagaagcac
    17941 caggtcgatt tcgtctcggg gatcatctgg tcgaacgtga tgatggcggt catgccggtc
    18001 gtcaccggcg cgggcaagat catggtcggc tcgaacgccg gcgcaagccc gctcgcgggc
    18061 agccagtgca acgagctcta tttctccacc tcgtggaaca acgaccagac gcccgaggcg
    18121 atgggcaagt tcctgcagga ccagggggtg aacgacatct acgtgatggc gccgaactac
    18181 caggccggca aggacatggt ggcgggcctc aagcgctact acaagggccg catcgtcgag
    18241 gagacctaca ccaagctcgg ccagcaggac taccaggccg agatcacgca gctgcgctcg
    18301 aagaacccga aggcggtgtt cgtgttctac ccgggcggca tgggcatcca gttcctgcgc
    18361 cagtacgacc aggccggcct gcacggcaag ctgccgctct acagcgtgta cacggtggac
    18421 gaaatctcga tcccggcagt gaaggacgcg gcgctcggtg tcaaggaggc gcgcttctgg
    18481 agcccggacc tgaagaacgc ggcgagcgag aagtacgtcg ccgactacaa gaaaaagtac
    18541 ggcaagctgc cggtgttcta cggcgcgcag agctacgacg ccatcatgct gatcgactcg
    18601 gcggtgcgcg gcaccaaggg caacctgaag gacacccagg cgctagtgaa ggaaatgcgc
    18661 aaggcgaact tcaattcggt gcgcggcaag ttcgagttca acacgaacca ccacccgatc
    18721 ca
//
LOCUS       DATBSZ010000190        16555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_784414, whole genome shotgun sequence.
ACCESSION   DATBSZ010000190 DATBSZ010000000
VERSION     DATBSZ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16555
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784414"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VIG70_12185"
     CDS             complement(<1..279)
                     /locus_tag="VIG70_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8251355.1"
                     /translation="MARKTPIERYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWKGMDMSYPEHHINIIDTPGHV"
     gene            complement(291..761)
                     /gene="rpsG"
                     /locus_tag="VIG70_12190"
     CDS             complement(291..761)
                     /gene="rpsG"
                     /locus_tag="VIG70_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY8251356.1"
                     /translation="MPRRREVPKREILPDPKFSNVDLAKFVNVLMTRGKKSVAERIIY
                     GALDTIKSKSGKDPIEVFTQAVQNVKPVVEVKSRRVGGANYQVPVEVRPVRRVALAMR
                     WIREAAQKRGEKSMMARLAGELAEAAEGRGGAMKKREEVHRMAEANKAFSHYRF"
     gene            complement(765..1142)
                     /gene="rpsL"
                     /locus_tag="VIG70_12195"
     CDS             complement(765..1142)
                     /gene="rpsL"
                     /locus_tag="VIG70_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY8251357.1"
                     /translation="MPTVNQLVREGRVAKRAKSKVPALAGSPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVIGYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGSL
                     DTQGVKDRKQGRSKYGAKAPKLA"
     gene            complement(1242..1721)
                     /locus_tag="VIG70_12200"
     CDS             complement(1242..1721)
                     /locus_tag="VIG70_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006416231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEY8251358.1"
                     /translation="MDEIVARSLAKWPNVPAVYGWLELDRRGNWLIKGERIGNQALRE
                     FIARNYEADERGRWFFQNGPQRVYVRLAYTPFVMHYEGGDLYDHCGRPTEALETFLDE
                     EGSVLIRCSHGIGLLDDRDLERYVDSADRLTRIARAEVEKRFGFVSDPANAAPDSLR"
     gene            1752..3413
                     /locus_tag="VIG70_12205"
     CDS             1752..3413
                     /locus_tag="VIG70_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY8251359.1"
                     /translation="MSAIETYLAKPWLAHYQKGVPAQVEVPLKSVAQAFDEATERAPE
                     RAAVVFYGRSISYRELREASDRLACALADLGVKKGDRVALYLLNSPQFIIAYFAALKC
                     GATVTPISPVYTSHEVRYQLEDSGARAIICQDILHEKVTKSGASLDLVVVTNVNEYLP
                     ALKRLFNKKAEVSGKVHWLQDLLKKYPAKPPAVTIDPRRDLAALPYTGGTTGNPKGVM
                     LTHYNLVSAQAMAQAAFSTFEPGKEVILAFLPFFHIYGQVVIMLQGLCQGNLLVLFTS
                     PDTEAILEAMERYRATVFYGVPTLYEYLKDHKDTNKTDWKRLKLVLSGADTLHESTMQ
                     GWAKRTGSNIIEGYGLSETCATSHVNPLHRPKTGSFGCPLPGMQAAVMNPDTLEFVPP
                     GETGELVLCADNVMQGYWQRREESARAFVEKGGVRWMRTGDLVRMDDEGYFHFYDRSK
                     DLIKFKGYSIFAKDVEDVLYAHPQVKAAGVIGVPDPAVGQRIKAIVVLQGDARGKLSE
                     DEIKAYCRQSLAEYKVPHIVEFRGELPKTDVGKVSRRELRDETKV"
     gene            complement(3415..4230)
                     /locus_tag="VIG70_12210"
     CDS             complement(3415..4230)
                     /locus_tag="VIG70_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8251360.1"
                     /translation="MPLEYLVDDKSLDQTSAELTGWPKPLARLREALEKSEFALYCQP
                     IAALAKDPGSRERYPMAEVLVRMREEERALLPPGEFLPVFEHYGMMPQLDRWVLRHTV
                     QQLARGSRIPRFTVNVSGQTMRDAGFPAFVAAELRAAGIAMDAVMFEIDESEALARPE
                     SAAAFASAVKAYGGGVLLDSFCHRAVSFAPIKALRPDFVKVDGSVIRKLLTNEGAQSK
                     LKAVVRVALALGIGVVAECVETPEVLARLKALDVGFAQGFGVYQPQPIEKVVG"
     gene            4229..5002
                     /locus_tag="VIG70_12215"
     CDS             4229..5002
                     /locus_tag="VIG70_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251361.1"
                     /translation="MPDAFFAVERLTKRFGGLTAVNNVSFTLARDEIVGLIGPNGAGK
                     TTLLRLITKILRPDAGRIAFNGEDVTGLRPWDIVERGIAGTFQNMRPFRHLPIVANVM
                     VPLVAPRARRRGEWVKSIEAKAMDALEFVGISDMALEPASALSQGDLKRLEVARAIAT
                     EPELLLLDEPLGGLSPAESELLAKSIKRLHKGGRFGRLHSEGPAVLMIEHKLKELMSI
                     VDRVIVIDYGEIIADGPPAEVVKNPKVIEAYLGSEHAAA"
     gene            4995..5738
                     /locus_tag="VIG70_12220"
     CDS             4995..5738
                     /locus_tag="VIG70_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251362.1"
                     /translation="MLEVKNLSVRYDKAQILNGVSLAVNAGEFVGLVGPNGAGKSTLL
                     RAISGLVRFEERMKRGTTGDIVLEGEVSFAGERIDGLAAHEIRMRGLVHCPERRRPFK
                     ELTVLENLLAGAFLSKNAKETKSRLDHCFALFPRLSERKGQIAGTLSGGEQQMLATAR
                     ALMYEAKLLAIDEPSLGLAPRIREELFAAIGKIHAEGVPVLLVEQEVGQVFRMAKRSY
                     VLSQGRIIAEGSAQSLMADETLRAGYLGL"
     gene            complement(5691..6788)
                     /locus_tag="VIG70_12225"
     CDS             complement(5691..6788)
                     /locus_tag="VIG70_12225"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8251363.1"
                     /translation="MYWLRRGWAELRNEVFVLPSRTLVLIWVVALLALPVVYGDPYVM
                     RILTMTCIFAIFAASWDLLAGYTGQVNFGHALFFGAGAYASALMSLKLGLSPWVTVWA
                     GAAVALLFGFVVGYLCLRLRGSYLSLATLAFPLIALGILFAFPDFSGGELGISGLRRL
                     MVTPTGNYYVAAVSMLAVVFALWLLADSKFGIVLHAIRDDEVAARASGINTLRYKMVV
                     FAISGAAAGFAGALFAHYLRVAGPSTLEVALSFQVVIWGIFGGVATIYGPVAAVFILY
                     PLTEWLGSFRAVGELRLLIFAIIVLLVLLFMPRGLSPWVRDKMETLCPRCKQRNGVWR
                     KICRLCGAGLSGRDPKLQAQVPGAKRLVGHQ"
     gene            complement(6788..7657)
                     /locus_tag="VIG70_12230"
     CDS             complement(6788..7657)
                     /locus_tag="VIG70_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8251364.1"
                     /translation="MLQDILVTGLVNGGVYALLAIGFSLIFGVARIVNIAHTAFYMLA
                     AYCFYTLLTRTGLGFLLSGLIAVGGVTLFSVLCYRLVIEPVREHEAAVLIATIALALV
                     FQELMLFTFGGHFLGIPSTLEGAVRFLGVSIPYQRLLILVVAGAMLAGVWFILYRTRL
                     GLAIRATANDLEVANLMGMNVNRVAMATVAISVSLAAVAGVVVAPVYVVDPFMWLAPL
                     VTMLAIVVLGGLGSLKGSLIGALIIGYVEAITVFAVPAGAYLKGAVALLIMVIVLLVR
                     PEGLFGVAFEEER"
     gene            complement(7665..8894)
                     /locus_tag="VIG70_12235"
     CDS             complement(7665..8894)
                     /locus_tag="VIG70_12235"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251365.1"
                     /translation="MRRSLTAGLAAFAFAAVATSGAQAQTIKIAILGPMAFVQGENHW
                     AGAEMARDEINKAGGVNVGGKRMQVELIRADTNEIQSVPDATNAIERVITRDKADFLI
                     GGFRSEAVLAMQEVAMDYKKIFLGAGAAHSKLGANVEANYDRYKYWFRVTPVKDVDLV
                     RTLFAVLGTIGQQIRTELKTDTPKVAILAEKAVWTEALVGAAQKNLPALKMEVVGTWQ
                     PSATATDVTAELSAIDRAGAHIVFTMLSGPVGISVGRQMGERNMKAVAWGINVEGQKD
                     EFWQAAAGKAQFVSTLDTYAEVEMTPKTIPFVQAFKQRYKKSPTYNAATYDAIMVLKV
                     AIEQAGTTNADKLVPVLEKLEHVGTGAIATWDKRHDLVWAVGKTAGIAVQWQDGKKVP
                     FWPPNVKGMQPFKMPAR"
     gene            8954..9394
                     /locus_tag="VIG70_12240"
     CDS             8954..9394
                     /locus_tag="VIG70_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY8251366.1"
                     /translation="MIWWEDFKVGEEAELGRHTFTEEEIVAFGRQYDPQPFHVDPEKA
                     RAGVFGGLIASGWHTCAVGMRLMCQGYINRTASLGSPGIDNIRWLKPVRAGDTVAYRR
                     IVLESRASMTRKGVGLVKHRWEAVNQHGELVLTMEGWGLFGRKN"
     gene            9378..10115
                     /locus_tag="VIG70_12245"
     CDS             9378..10115
                     /locus_tag="VIG70_12245"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEY8251367.1"
                     /translation="MAARIDLNHKVHVLTKKEELDSVRVPGKVVVVLDILFATTTMVA
                     ALAHGAKEVIPVVDESAARTQAQEFEKGLYVLAGELYAETLPGFAHPAPLSLLEHGVE
                     GKSLIYSTTNGTVAMMQPARGARVYCGALLNARALAAHIVARHPRETVLIVCSGSGNN
                     FNFEDFYGAGYFVECFLEHLGDLSDAAKAALTLYRHAKAPEALLDCRVGRMMVARGLA
                     REVEFACRRDAFPVVPALEDGRLRLVT"
     gene            10115..10828
                     /locus_tag="VIG70_12250"
     CDS             10115..10828
                     /locus_tag="VIG70_12250"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEY8251368.1"
                     /translation="MRVAGLYVYPVKACRAVAIEDAALGPLGLEHDRRFAFIGEDGCA
                     LTQREHPLLATVQAALGKDSLRLDFGSLAQVNIPLKSFVTSIPVDVWGKRIAGRAAPA
                     SLVAPAADYLGTRLQLIALDAAEERSFADSRPVLVATTGMLARLDLPDVGMERFRPNV
                     VLEGERDWTALEGKDVLLERDKPCGRCEVTTIDQASGARCGPEPLRTLTERFEGNFGV
                     YCRVARGGRLRRGEILRAS"
     gene            complement(10825..11577)
                     /locus_tag="VIG70_12255"
     CDS             complement(10825..11577)
                     /locus_tag="VIG70_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY8251369.1"
                     /translation="MTKNRLRIVTLNIHKGLSQFNRRLVIHELRDGLRALDPDLVFLQ
                     EVQGLNQRFALRFAGAPVDPQHAFLAGDDWQHVYGCNAVYDHGHHGNAILSRFGFLSF
                     ENKDVSDHRYERRGLLHSVVAVPGWRRNLHCVCVHLSLHERGRSRQLEAISGRLEELA
                     SRDLPIIIAGDFNDWRHRATAILERRLGMTEATVSRHGRVLATYPSLLPILRLDRIYV
                     RGFKVVAAQVHRGKPWSLLSDHLAVSAELERA"
     gene            complement(11574..13436)
                     /gene="nudB"
                     /locus_tag="VIG70_12260"
     CDS             complement(11574..13436)
                     /gene="nudB"
                     /locus_tag="VIG70_12260"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEY8251370.1"
                     /translation="MKLPRSVLVVVHTAEREVLLLERAARPGFWQSVTGSLEHPDEPY
                     EVAAARELREETGIEAAAVSLRRWEVAYTFEIYAQWRHRFAPGVSHNTERLFSLELSG
                     RAPVALAREEHTAFAWLPWRAAARKCFSWSNRDAILMIGAGLLVAGCATGDGRLAAHL
                     ESGPPAVAECAQWYRALDAEIDAAGVRDAQHTRVPGFPHLRVDRTLASLKSRAAESQL
                     GLRAFGERLAELDLEARHHETRNLPALNENARTLMLKRARDCSSLLRDADLVSSGGRE
                     ALVAAAQVPDDYSTGQRVVGLYALTKLAFVSSIRRWEKETLAAFEREADQAASRVRYS
                     PPSAQHLPRNAVAGLLGRATFDPLGHPGLSARELDRLAMAYAPSFEIEIRGDHDRFGW
                     LRWRRASGVPEVDAAEPTVYTHAVYTRYRDQVLLQLVYTVWFPERPPVGAVDLLAGRL
                     DAVVWRVTLAPDGEPVVYDSIHACGCFHLFFPTERARLRSPPDSLDEWAFVPQSLPRV
                     GEDERPVVTLASGTHAIERVTMARGGDSVVRYAMRPYDELRSLPRAGGVHASAFGPDG
                     LIAGTERAERFFFWPTGVASAGAMRQWGRHATAFVGRRHFDDADLLERRFDLDL"
     gene            complement(13433..13837)
                     /locus_tag="VIG70_12265"
     CDS             complement(13433..13837)
                     /locus_tag="VIG70_12265"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEY8251371.1"
                     /translation="MGKLIERLQDASRSGVYRAARAEEITHAVDGGRLSLARIRFADK
                     ETLLKNIAAALEFPDWFGANWDALEDCLSDLPGEGYVVLFENPKASDEFGILVDVLRS
                     SAESWAGRGKPFFAVFIDPGRRLALPDLFTEK"
     gene            complement(13837..14166)
                     /locus_tag="VIG70_12270"
     CDS             complement(13837..14166)
                     /locus_tag="VIG70_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HEY8251372.1"
                     /translation="MSWLAVAVVAFALSTEVPIEKLPPEARETIALIKAGGPFPYSQD
                     GKTFQNREKILPRKERGYYREYTVKTPGAKDRGARRIVGGRCGEYGCVEYYYTDDHYR
                     SFRRIIE"
     gene            complement(14163..15938)
                     /gene="aspS"
                     /locus_tag="VIG70_12275"
     CDS             complement(14163..15938)
                     /gene="aspS"
                     /locus_tag="VIG70_12275"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY8251373.1"
                     /translation="MRSHYCGEMRATLVDRTVTLCGWAHRRRDHGGVIFIDLRDREGL
                     AQVVCDPDRQEAFRIAERVRSEYVLRVTGKVRRRPAGTVNPELPTGEVEVLAHEVEIL
                     NPSAPPPFQIDEENLSEETRLQHRILDLRRAPMQRNLRLRHRAAMAVRRFLDEHGFVD
                     IETPVLYKSTPEGAREFLVPSRLHEGHFYALPQSPQLFKQMLMVAGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDIETSFLSENDIRALMESLVRFVFRETIGVELPAPFPVLTYDE
                     AMRDYGSDKPDLRIALKLAEITDAVKDVEFKVFAGPANAAGGRVCALRVPGGGALTRG
                     ELDAYGEYAKTLGAKGLAWIKVNERAKGAEGLQSPIVKNLHAASIGAILDRTAAQSGD
                     VILFGAGDAATVDAYMGALRDKVGHDRGLAEKGWRPLWVVDFPMFEQDPQTREWKARH
                     HPFTSPKDGHEQYIEAAPGKAYAKAYDMVLNGWEIGGGSVRIHRAEVQAKVFEAMRIT
                     KEEQRAKFGFLLDALQYGAPPHGGIAFGFDRLVTLMAGAEAIRDMIAFPKTQRGQDLL
                     TGAPTSVTEKQLRELHIRLRNPPQP"
     gene            complement(15938..16528)
                     /locus_tag="VIG70_12280"
     CDS             complement(15938..16528)
                     /locus_tag="VIG70_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEY8251374.1"
                     /translation="MKKYFITGLLIWIPLAITIWVLKLVVDVLDQSLHLLPAALHTEN
                     WLGVHIPGLGAILTVLIVFLTGVFATNFFGAQLVELWHEILHRIPVVNSIYSSVKQIS
                     DTLFSSSGQAFRKALLVQWPHEGMWTIAFLTGTPGGGVANHLPPDCVAVYVPTTPNPT
                     GGYFVIIARKNVIELDMSVDQALKYIISMGVVPPQK"
BASE COUNT         2852 a         5527 c         5507 g         2669 t
ORIGIN      
        1 gacgtggccc ggggtatcga tgatgttgat gtggtgttcc ggataggaca tgtccatgcc
       61 cttccagaaa caggtggtgg ccgcggaagt gatggtgatg ccgcgctcct gctcctgctc
      121 catccagtcc atcgtcgcgg cgccgtcgtg cacttcgccg atcttgtgat tgacgccggt
      181 gtagaacagg atgcgctcgg tcgtggtcgt ctttccggcg tcgatgtgcg ccgaaatgcc
      241 gatgttccga taacgctcga tgggggtttt gcgggccacg ttcatttttc ctagaacctg
      301 tagtgcgaga aggccttgtt ggcctctgcc atgcggtgca cttcctcgcg cttcttcatc
      361 gcgccgccgc gaccttccgc cgcctcggcc aactcgccgg caaggcgcgc catcatcgac
      421 ttctcgccgc gcttctgcgc cgcctcgcgg atccagcgca tcgcgagcgc cacgcggcgc
      481 accggccgca cttccaccgg cacctggtag ttggcaccgc cgacgcgccg gctcttcact
      541 tccaccaccg gcttcacgtt ctgcaccgcc tgcgtgaaca cctcgatcgg gtccttaccc
      601 gacttcgatt tgatggtgtc gagggcgccg taaatgatgc gctcggcgac cgacttcttg
      661 ccgcgcgtca tcagcacgtt gacgaacttg gcgaggtcga cgttgctgaa cttcggatcc
      721 ggcaggatct cgcgcttggg aacttctctg cgtcgtggca tggtttaagc caatttcggc
      781 gccttggcgc cgtatttcga gcggccctgc ttgcggtcct tcacgccctg cgtgtcgagc
      841 gagccgcgca cgatgtggta gcgcacgccg ggcaggtcct tgacgcgccc gccgcggatc
      901 agcaccaccg agtgctcctg cagattgtgg ccttcgccgc cgatgtaacc gatcacctcg
      961 aagccgttgg tgaggcggac cttcgccact ttgcgcagcg ccgaattcgg cttcttgggc
     1021 gtggtcgtat agacccgcgt gcagaccccc cgcttctgcg gcgagccggc gagcgccggg
     1081 actttcgact tggcgcgctt ggcgacgcgc ccttcacgca ctaactggtt gacggtgggc
     1141 atgttttagc gtttccagaa acgaacaggc ggccccgttt taagctcccc gtaggccgcc
     1201 cagacccaaa aaagaggccg gattataaag aggaaattac ctcaacgcaa ggaatccggg
     1261 gctgcgtttg cagggtcgga gacgaagccg aagcgctttt ccacctcggc gcgcgcgatg
     1321 cgcgtcaatc ggtctgccga atcgacgtag cgctcgaggt cacggtcgtc cagtagtccg
     1381 atgccgtggc tgcagcggat cagcaccgac ccctcttcgt cgagaaacgt ctcgagcgct
     1441 tcggttggtc gcccgcaatg gtcgtacagg tccccgccct cgtagtgcat cacgaatggc
     1501 gtgtaggcca gcctcacgta gacccgctgc gggccgttct gaaaaaacca gcggccacgc
     1561 tcgtcggctt cgtagttgcg ggcgatgaac tcgcgcaagg cttgattgcc gatgcgctcg
     1621 cccttgatca gccagttgcc gcgccggtcg agctcgagcc agccgtacac tgccggaacg
     1681 ttgggccatt tggcgaggct gcgggcgacg atttcgtcca tcgagggata gaatcttcgg
     1741 cgggaggcgg catgagcgca atcgagacgt acctggcgaa gccctggctc gcgcattacc
     1801 agaagggtgt gcctgcccag gtagaagttc cgctcaagtc ggtggcgcag gccttcgacg
     1861 aggcgaccga gcgcgccccg gagcgcgccg cggtagtgtt ctacggccgc tcgatcagct
     1921 accgcgagct gcgcgaggcc agcgaccgcc tcgcctgcgc gctcgccgac ctcggcgtga
     1981 agaagggcga ccgcgtcgcg ctctacctgc tcaacagccc ccaattcatc atcgcgtact
     2041 tcgcggcgct caagtgcggc gcgaccgtga ctccgatcag cccggtctac accagccacg
     2101 aggtgcgcta tcagctggag gacagcggcg cgcgcgccat catctgccag gacatcctgc
     2161 acgagaaagt gaccaagtcc ggcgcctcgc tcgacctcgt ggtggtgacg aacgtcaacg
     2221 aatacctgcc cgctctaaag cgccttttca acaagaaagc cgaggtttcc ggaaaggtgc
     2281 actggctgca ggacctgctg aagaaatatc ccgcgaagcc tccggccgtg acgatcgacc
     2341 cgaggaggga cctggcggcg ctgccctaca cgggcggcac gaccggcaac ccgaaggggg
     2401 taatgctcac gcattacaac ctggtctcgg cgcaagccat ggcgcaggcg gcgttctcga
     2461 ccttcgagcc gggcaaggaa gtgatcctcg cgttcctgcc gttcttccac atctatggcc
     2521 aggtcgtgat catgctgcag ggcctgtgcc aggggaacct gctggtgctg ttcaccagcc
     2581 cggacaccga ggcgatcctc gaggcgatgg agcgctaccg ggcgacggtg ttctacggcg
     2641 tgccgacgct ctacgaatat ctcaaggacc acaaggacac caacaagacc gactggaagc
     2701 gcctcaagct cgtgctgtcg ggcgccgaca cgctgcacga gtcgacgatg cagggctggg
     2761 cgaagcgcac cgggtccaac atcatcgagg gctacggcct gtcggagacc tgcgccacga
     2821 gccacgtcaa cccgctgcac cggccgaaga ccggctcgtt cggctgcccg ctccccggca
     2881 tgcaggccgc ggtgatgaat cccgacacgc tcgagttcgt gccgccgggc gagaccggcg
     2941 aactcgtgct atgcgccgac aacgtgatgc agggctactg gcagcggcgc gaggagagcg
     3001 cgcgcgcgtt cgtcgaaaaa ggcggcgtgc gctggatgcg caccggcgac ctggtgcgca
     3061 tggacgacga gggttacttc catttctacg accgctccaa ggacctgatc aagttcaagg
     3121 gttactcgat cttcgccaag gacgtcgagg acgtgctgta cgcccatccc caggtcaagg
     3181 cggcgggagt gatcggcgtt ccggatccgg cggtcggaca gcgcatcaag gcgatcgtgg
     3241 tgctgcaggg ggacgcgcgc ggcaagctgt ccgaggacga gatcaaggct tattgccggc
     3301 aaagcctcgc cgagtacaag gtgccgcaca tcgtcgagtt ccgcggcgag ctgcccaaga
     3361 ccgacgtggg caaggtctcg cgccgcgagc tgcgcgacga aaccaaggtc taggtcagcc
     3421 cacgaccttc tcgatcggct gcggctgata gacgccgaag ccctgggcaa agccgacgtc
     3481 gagcgctttc aggcgcgcga gcacctccgg cgtctcgacg cactcggcga ccacgccgat
     3541 gccgagcgcc agcgcgaccc gaacgaccgc cttgagcttg ctctgcgccc cctcgttggt
     3601 gaggagcttg cggatgacgc tgccgtcgac cttgacgaag tcgggccgca gggccttgat
     3661 cggcgcgaac gacacggcgc ggtggcagaa gctgtcgagg agcacgccgc cgccataggc
     3721 cttgaccgcc gaggcgaagg ccgccgcgga ctcgggccgc gcgagcgcct cgctctcgtc
     3781 gatctcgaac atcaccgcgt ccatcgcgat gccggcggcg cgcagctcgg cggcgacgaa
     3841 cgccgggaac ccggcgtcgc gcatcgtctg gcccgaaacg ttgacggtga agcgcggaat
     3901 gcgcgagccg cgcgcgagct gctgcaccgt gtggcgcagc acccaccggt caagctgcgg
     3961 catcattccg tagtgctcga acaccggcag gaactctccc ggcggcagca gcgcgcgctc
     4021 ctcctcgcgc atgcgcacca gcacttcggc catcgggtag cgctcgcggc tgccggggtc
     4081 cttggccagg gcggcgatcg gctggcaata gagcgcgaac tcgctcttct ccagcgcttc
     4141 gcgcaggcgc gcgagcggct tcggccagcc ggtgagctca gcgctcgtct ggtcgagcga
     4201 cttatcgtcc acgaggtatt ctagcggcat gccggacgcc ttcttcgcgg tcgagcgact
     4261 taccaagcgc ttcggcggcc tcaccgccgt caacaacgtg agcttcacgc tcgcgcgcga
     4321 cgagatcgtc gggctgatcg gcccgaacgg cgcgggcaag acgacgttgc tgcgcctcat
     4381 caccaagatc ctgcgtcccg acgccgggcg catcgcgttc aacggcgagg acgtcaccgg
     4441 gctgcgtcct tgggacatcg tcgagcgcgg catcgcgggc acgttccaga acatgcggcc
     4501 gttccgccat ctgccgatcg tggcgaacgt gatggtgccg ctggttgcgc cgcgcgcgcg
     4561 ccgccgcggc gaatgggtga aaagcatcga ggccaaggcg atggacgcgc tggaattcgt
     4621 cggcatctcc gacatggcgc tcgagccggc ctcggcgctc tcgcagggag atctcaagcg
     4681 cctcgaggtg gcgcgcgcca tcgccaccga gcccgagctc ctgctgctcg acgagccgct
     4741 cggcgggctg agcccggcgg aatcggagct gctcgcgaag tcgatcaagc gcctgcacaa
     4801 gggcgggcgc ttcgggcgcc tgcattccga aggccccgcg gtgctgatga tcgagcacaa
     4861 gctgaaggag ctgatgtcga tcgtcgaccg cgtgatcgtg atcgactacg gcgagatcat
     4921 cgccgacggt ccgccggccg aagtcgtgaa gaatccaaaa gtgatcgagg cgtacctggg
     4981 aagcgaacat gccgctgctt gaggtgaaga acctcagcgt gcgctacgac aaggcgcaga
     5041 tcctgaacgg cgtctcgctc gcggtgaacg cgggcgaatt cgtcggcctg gtcggaccga
     5101 acggcgccgg caaatcgacc ctgctgcgcg cgatctcggg cctggtgcgc ttcgaggaac
     5161 gcatgaagcg cggcaccacc ggcgacatcg tgctcgaggg cgaggtgagc ttcgccggcg
     5221 agcgcatcga cggcctcgcc gcgcacgaga tccgcatgcg gggactggtg cactgcccgg
     5281 agcggcgccg tccgttcaag gaactgacgg tactggagaa ccttctcgcc ggcgcttttc
     5341 tctcaaagaa tgccaaagag acgaaaagcc gcctcgatca ctgcttcgcg ctgtttccgc
     5401 gattgtccga gaggaagggc cagatcgccg gcactctgtc gggcggcgag cagcagatgc
     5461 tcgccacggc gcgcgcgctg atgtacgagg ccaagctcct cgcgatcgac gagccttcgc
     5521 tcggactcgc gccgcgcata cgcgaggagc tgttcgccgc gatcggcaag atccacgccg
     5581 aaggcgtacc ggtgctgctg gtcgagcagg aagtcgggca ggtgttccgg atggcgaaac
     5641 gcagctacgt gctgtcgcag gggcgcatca tcgccgaggg ctcggcgcag tcactgatgg
     5701 ccgacgagac gcttcgcgcc gggtacctgg gcttgtagtt tcgggtcccg tcccgacaag
     5761 ccggcgccgc acagacggca gatctttcgc cacaccccgt tgcgctgctt gcagcgcggg
     5821 cacagggtct ccatcttgtc gcgcacccat ggagaaagac cgcgcggcat aaagagcagc
     5881 accagcagca cgatgatcgc gaagatcagc aggcgcagct cgccgactgc gcggaagctg
     5941 ccgagccact cggtgagcgg atagaggatg aacacggcgg ccaccggccc atagatcgtg
     6001 gcgacgccgc cgaagatccc ccagatcacc acctggaagg agagcgccac ttcgagcgtc
     6061 gacggtcccg ccacgcgcag gtagtgcgca aacagagccc cggcgaaacc ggcggccgcg
     6121 ccggagatcg cgaagacgac catcttgtag cgcagcgtat tgatgcccga ggcgcgcgcc
     6181 gccacttcgt cgtcgcggat cgcgtgcaat acgatgccga acttcgaatc agccaatagc
     6241 cacaatgcga agaccacggc gagcatcgag accgcggcga cgtagtagtt gcccgtgggc
     6301 gtcaccatca ggcgccgcag ccccgagatg ccgagctcgc cgccggagaa atccgggaag
     6361 gcgaacagga tcccgagcgc gatcagcggg aaggccagag tggcgaggga gagataggag
     6421 ccgcgaagac gcaggcagag gtagcccacg acaaaaccga aaagcagagc gaccgcggcg
     6481 ccggcccaga ccgtgaccca gggcgaaagg ccgagcttga gcgacatgag cgccgaggca
     6541 taggcgccgg cgccgaagaa cagcgcgtgg ccgaaattca cctgcccggt gtatcccgcg
     6601 agcaggtccc agctcgcggc gaagatcgcg aagatgcagg tcatcgtcag gatgcgcatc
     6661 acgtagggat cgccgtaaac caccggcaac gcgaggagcg cgacgaccca gatcagcacc
     6721 agcgtacgcg acggcaggac gaacacttca ttgcgcagct cggcccaccc gcgtcgaagc
     6781 caatacatca gcgctcctcc tcgaacgcga caccaaacag gccttcaggc cgcacgagca
     6841 acacgatcac catgatgagg agcgccaccg cacccttgag atacgcgccg gcgggaaccg
     6901 cgaagaccgt gatcgcctcg acgtagccga tgatcagcgc gccgatcagg ctgcccttca
     6961 ggctgcccag gccgccgagc accacgatcg cgagcatggt gaccagcggc gcgagccaca
     7021 tgaacgggtc gaccacgtac accggcgcca ccactacgcc ggcgaccgca gcgagcgaca
     7081 ccgagatcgc caccgtcgcc atggcgacgc gattcacgtt catgcccatc aggtttgcga
     7141 cctcgaggtc gttggccgtg gcccggatcg ccaggccgag ccgggtgcgg tagaggatga
     7201 accagacccc ggcgagcatg gcaccggcca ccaccaggat cagaagccgc tggtacggga
     7261 tgctcacgcc cagaaagcgc accgcgcctt cgagcgtcga cgggatgccg aggaaatgcc
     7321 cgccgaacgt gaacagcatc agctcctgga acaccagcgc gagcgcgatg gtcgcgatca
     7381 gcaccgccgc ctcgtgctcg cgcaccggct cgatcaccag gcggtagcag agcaccgaga
     7441 acaaggtcac gccgccgacc gcaatcaggc ccgagagcag gaagccgagg ccggtcctgg
     7501 tcagcagcgt gtagaagcag tacgccgcca gcatgtagaa cgcggtgtgc gcgatattga
     7561 cgatgcgcgc gacgccgaag atcagcgaga agccgatcgc cagcagggcg tacacgcccc
     7621 cgttgacgag gcctgttaca agaatgtctt gtaacaaagg cgcgctagcg cgccggcatc
     7681 ttgaagggct gcatgccctt tacgttcggc ggccagaacg gcactttctt gccgtcctgc
     7741 cactgcaccg cgatgccggc cgtcttgccc actgcccaga cgaggtcgtg gcgcttgtcc
     7801 caggtcgcga tcgcgcccgt cccgacgtgc tcgagcttct ccagcaccgg caccagcttg
     7861 tcggcgttgg tggtgccggc ctgctcgatc gccaccttca gcaccatgat cgcgtcgtag
     7921 gtcgccgcgt tgtaggtggg cgacttcttg tagcgctgct tgaacgcctg cacgaagggg
     7981 atggtcttcg gcgtcatctc gacctcggcg taggtgtcga gcgtcgacac gaactgcgcc
     8041 ttgccggcgg cggcttgcca gaactcgtcc ttctgtcctt cgacgttgat gccccaggcc
     8101 accgccttca tgttgcgctc gcccatctgg cggcccaccg agatgccgac cgggcccgac
     8161 agcatggtga acacgatgtg cgcgccggcg cggtcgatcg ccgaaagctc cgcggtcacg
     8221 tccgtggcgg tggcggacgg ctgccaggtc ccgaccactt ccatcttgag cgccggcagg
     8281 ttcttctgcg ccgcgccgac cagcgcctcg gtccacaccg ccttctcggc gaggatcgcg
     8341 accttgggcg tgtcggtctt gagctcggtg cgaatctgct ggccgatcgt gcccagcacg
     8401 gcgaacagcg tgcggaccag atcgacgtcc ttcaccgggg tgacgcggaa ccagtacttg
     8461 tagcggtcgt agttcgcttc gacgttggcg ccgagcttgg agtgcgccgc gcccgcgccg
     8521 aggaagatct tcttgtagtc catcgccact tcctgcatcg cgagcaccgc ctcggagcgg
     8581 aagccgccga tcaggaagtc ggccttgtcg cgcgtgatga cgcgctcgat ggcgttggtt
     8641 gcgtcgggta ccgactggat ctcgttggtg tcggcgcgga tcagctcgac ctgcatgcgc
     8701 ttgccgccga cgttgacgcc gcccgccttg ttgatctcgt cgcgcgccat ctccgcgccc
     8761 gcccagtggt tctcgccttg cacgaaagcc atggggccga ggatcgcgat cttgatggtc
     8821 tgcgcctgag cgcccgaagt cgcaactgcg gcgaacgcga atgccgccag ccctgccgtc
     8881 aacgaacgcc gcatgccctg cctccttttt gaactacact tatcttgttg tattcaagga
     8941 tgttgccagc aatttgatct ggtgggaaga cttcaaggtc ggcgaggaag ccgagctcgg
     9001 gcggcacacc ttcaccgagg aggagatcgt cgccttcggc aggcagtacg atccgcagcc
     9061 ttttcacgtc gatcctgaga aggcaagagc gggggtcttc ggcggcctga tcgccagcgg
     9121 ctggcatacc tgcgccgtgg gaatgcgcct catgtgccag ggctacatca accgcaccgc
     9181 gagcctcggc tcgcccggca tcgacaacat ccgctggctg aagccggtgc gcgccggcga
     9241 caccgtcgct tatcgccgca tcgtgctcga gtcgcgcgcc tcgatgacgc gcaaaggagt
     9301 cggcctggtc aagcatcgct gggaggcggt gaaccagcac ggcgagctcg tgctcacgat
     9361 ggaaggctgg gggctttttg gccgcaagaa ttgatctcaa ccacaaggtc catgtgctca
     9421 ccaagaagga ggagctggac agcgtgcgcg tgccgggcaa ggtcgtcgtg gtactcgaca
     9481 tcctgttcgc gaccaccacc atggtcgccg cgctcgcgca cggcgcgaaa gaagtgattc
     9541 ccgtagtgga cgagtccgcc gcccgcactc aagcacagga gttcgagaaa ggcttgtacg
     9601 tacttgccgg cgagctgtac gcggaaacgc ttcccggatt cgctcacccg gcgccgcttt
     9661 ccttgctcga gcatggcgtc gaaggaaaga gcctcatcta ctcgaccacg aacggcaccg
     9721 tcgccatgat gcagccggcg cgcggggcgc gggtctattg cggcgccctc ctcaacgcgc
     9781 gcgcgctggc cgcccacatc gtggcgcggc acccgcgcga gaccgtgctc atcgtctgct
     9841 cgggctcggg caacaacttc aacttcgagg acttctacgg cgcaggctac ttcgtcgaat
     9901 gctttctcga gcacctggga gatctttcgg acgcggcaaa agccgccctg accctctacc
     9961 gccatgccaa ggcacccgaa gcgctgctcg attgccgcgt cggccgaatg atggttgcgc
    10021 gcggcctggc gcgcgaggtg gagttcgcct gccggcgcga cgcgttcccg gtcgtgccgg
    10081 cgctggagga cgggcgcctg cggctggtca cctgatgcgc gtcgccgggt tgtacgtcta
    10141 tccggtcaag gcctgccgcg ccgtcgccat cgaggacgcc gcgctcggac cgctcgggct
    10201 ggagcatgac cggcgtttcg cctttatcgg cgaagacggc tgcgcgctaa cccaacgcga
    10261 gcacccgctt ctcgccacgg tgcaggcggc gctcgggaag gattcgctgc ggctcgactt
    10321 cggcagcctg gcgcaggtga acatcccctt gaagagcttt gttacgagca tcccggttga
    10381 cgtgtgggga aagcgcatcg cggggcgcgc cgcgccggca tctcttgtgg cgcccgcggc
    10441 cgactatctc ggcacgcggt tgcagctcat tgccctggac gccgccgagg aacgctcgtt
    10501 cgcggactcg cggccggtgc tggtggcgac gacggggatg ctcgccaggc tcgatctgcc
    10561 cgatgtcggc atggagcgct tccggccgaa tgtcgtgctc gaaggcgagc gggattggac
    10621 tgcgctggag ggaaaggacg tactcctcga acgcgacaag ccctgcggcc gctgcgaagt
    10681 cacgaccatc gaccaggcga gcggcgcgcg ctgcggcccg gagccgctgc gcacgctcac
    10741 cgagcgcttc gaaggcaact tcggcgtcta ctgccgcgta gcgcgaggcg gccggctgcg
    10801 gcgcggcgag atcctgcgag cgtcctaggc tcgctcgagc tcggcgctca ccgcgaggtg
    10861 gtccgagagc agggaccacg gcttgccgcg atgcacctgt gccgccacga ccttgaaacc
    10921 gcgcacgtag atgcggtcga gccgcaggat cggcagcagg ctcgggtagg tcgcaagcac
    10981 ccggccgtgg cgcgatacgg tcgcctccgt catgcccagg cgccgctcga ggatcgcggt
    11041 ggcgcgatgg cgccagtcgt tgaaatcgcc ggcaatgatg atcggcaggt cgcgcgaggc
    11101 gagctcttcc agccgcccgg aaatcgcctc gagctggcgg ctgcggccgc gctcgtgcag
    11161 cgaaaggtgc acgcacacgc aatgcaggtt gcgccgccag ccggggaccg ccaccacgct
    11221 gtgcagcagg ccgcgacgct cgtagcggtg gtccgagacg tccttgtttt cgaacgacag
    11281 gaagccgaag cgcgacagga tcgcgttgcc gtggtggccg tggtcgtaga ccgcgttgca
    11341 gccgtagacg tgctgccagt cgtctccggc aaggaaagcg tgctgcgggt cgaccggggc
    11401 gccggcgaag cgcagcgcga agcgctggtt caggccctgg acttcctgca ggaatacgag
    11461 gtccggatcg agcgcgcgca ggccgtcgcg cagctcgtgg atcaccaggc gccggttgaa
    11521 ctgcgacagg cccttgtgga tgttgagggt gacgatgcgc aggcgattct tagtcataga
    11581 tccagatcga agcgccgctc gaggaggtcc gcatcgtcga agtggcggcg cccgacgaaa
    11641 gccgtggcat gccgtcccca ctggcgcatc gcgccggccg aggcgacgcc cgtcggccag
    11701 aagaagaagc gctcggctcg ctcggtgccg gcgatcagcc cgtcggggcc aaaggcgctg
    11761 gcatggaccc cgccggcgcg cggcagcgag cgcagctcgt catacgggcg catcgcgtag
    11821 cgcaccacgc tgtcgccgcc acgcgccatc gttacgcgct cgatcgcgtg cgtgccggag
    11881 gcgagcgtca ccaccgggcg ctcgtcttct ccgacccgcg gcagcgattg cggcacgaac
    11941 gcccattcgt cgagggaatc cggcggcgag cgcagtcgcg ccctctcggt cggaaagaaa
    12001 agatggaagc agccgcacgc atggatcgag tcatagacca ccggctcgcc gtcgggcgcg
    12061 agggtcacgc gccacaccac tgcgtccagc cggccggcaa gaagatcgac cgcgcccacg
    12121 ggcgggcgtt ccggaaacca gaccgtgtag accagctgaa gcagcacctg gtcgcggtag
    12181 cgcgtgtaca ccgcgtgcgt gtagaccgtc ggctccgcag cgtcgacctc gggtacgccg
    12241 gaggcgcggc gccagcgcag ccagccgaag cggtcatggt cgccgcggat ctcgatctcg
    12301 aagctgggcg cgtacgccat cgccagccgg tcgagctcgc gcgccgacag gccgggatgc
    12361 ccgagcggat cgaaagtcgc gcgcccgagc aggcccgcga ccgcgttccg cggcaagtgc
    12421 tgcgccgaag gcggggaata ccgcacgcgg ctcgcagcct gatcggcctc gcgctcgaaa
    12481 gcggccagcg tttccttctc ccagcgcctg atgctcgaga cgaaggcgag cttcgtcagc
    12541 gcatagagtc ccacgacgcg ctgtccagtc gagtaatcgt ccggtacctg cgcggccgcg
    12601 accaacgcct cgcgcccgcc ggagctcacg aggtcggcgt cgcgcagcag gctgctgcaa
    12661 tcgcgcgcac gcttgagcat cagggtgcgc gcgttctcgt tcagcgccgg caggttgcgg
    12721 gtctcgtgat gccgcgcctc gagatcgagc tcggcgaggc gctcgccgaa ggcgcgcagt
    12781 ccgagctggc tctccgcggc gcggctcttg agcgaggcga gcgtgcggtc tacgcgcagg
    12841 tggggaaagc ccggcacgcg agtgtgctgc gcatcgcgca cgcccgcggc gtcgatctcg
    12901 gcgtcgagcg cccggtacca ctgcgcgcac tcggcgactg cgggcggtcc ggactcgagg
    12961 tgcgcggcaa gccggccgtc gcccgtggcg cagccggcga cgagaagccc ggcgccgatc
    13021 atgaggatcg cgtcgcggtt ggaccaggag aagcacttgc gcgccgccgc gcgccacggc
    13081 agccaggcga aggccgtgtg ctcctcgcgc gcgagcgcta ccggcgcgcg cccggaaagc
    13141 tcgaggctga acaaccgctc ggtgttgtgg ctcacgccgg gagcaaagcg atgacgccat
    13201 tgcgcgtaaa tctcgaaggt ataggcgacc tcccagcgcc tcaggctgac ggcggccgct
    13261 tcgattccgg tttcctcgcg cagctcgcgc gccgccgcga cctcgtaggg ctcgtcggga
    13321 tgctcgagcg agcccgtgac cgactgccag aacccgggcc gcgctgcgcg ctcgagcagc
    13381 agcacctcgc gctcggcggt atgcacgacg accaggaccg accgcggcag tttcatttct
    13441 cggtgaacag gtcgggcagc gccaagcgcc gcccggggtc gatgaagact gcgaagaacg
    13501 gcttgccgcg cccggcccag gactccgcgc tcgagcgcag cacgtcgacc aggatgccga
    13561 actcgtcgct cgccttggga ttctcgaaca gcacgacata cccctccccg gggagatccg
    13621 agaggcagtc ttccagcgcg tcccagttgg cgccgaacca gtctgggaac tcgagcgccg
    13681 cagcgatgtt cttcaggagc gtttccttgt ctgcaaatcg aatcctcgca agagaaagcc
    13741 tgcccccgtc taccgcgtgc gtgatctctt cggcacgcgc cgcccggtac acgccggagc
    13801 gcgacgcatc ctgcagccgc tcgatcagct ttcccattac tcgatgatgc gcctgaagct
    13861 gcggtagtga tcgtcagtgt agtagtactc gacacagccg tactcgccac agcgcccgcc
    13921 cacgattcgc cgcgcgccgc gatccttcgc acccggggtt ttcaccgtgt actcgcggta
    13981 gtagccacgt tctttcctgg gaaggatctt ctcgcgattt tgaaacgtct tgccatcctg
    14041 ggagtaggga aacggtccgc ctgccttgat gagcgcgatc gtttcgcgag cctccggtgg
    14101 cagtttctca atgggaacct cggtggaaag cgcaaaagcc accaccgcca ccgcaagcca
    14161 actcacggct gcggcggatt cctcaggcgg atgtgcagct cgcgcagctg cttctcggtg
    14221 acggaagtcg gcgcgccggt gagcaggtcc tggccgcgct gggtcttcgg gaacgcgatc
    14281 atgtcgcgga tcgcctccgc gcccgccatc agggtcacca ggcggtcgaa gccgaaggcg
    14341 atgccgccgt gcggcggcgc gccgtactgg agcgcgtcga gcagaaaacc gaacttggcg
    14401 cgctgctcct ctttcgtgat tctcattgct tcaaaaactt ttgcctgaac ctcggcgcgg
    14461 tgaatgcgca ccgagccgcc gccgatctcc cagccgttca acaccatgtc gtaggccttg
    14521 gcgtacgcct ttccgggcgc cgcctcgatg tattgctcgt ggccgtcctt ggggctggtg
    14581 aacggatggt ggcgcgcctt ccactcgcgg gtctgcgggt cttgctcgaa catcgggaaa
    14641 tcgaccaccc acagcggccg ccatcccttc tcggcgagac cgcggtcgtg cccgaccttg
    14701 tcgcgcagcg cgcccatgta cgcgtcgacc gtggcggcgt cgccggcgcc gaaaaggatg
    14761 acgtccccac tctgggccgc ggttcgatcc aggatcgcac cgatcgatgc cgcgtgaaga
    14821 ttcttcacga tgggcgactg caggccctcg gcgcccttgg cgcgctcgtt caccttgatc
    14881 cacgcgaggc cctttgcccc aagcgtcttg gcgtattcgc cgtacgcgtc gagctcgccg
    14941 cgggtgagcg ccccgccgcc gggcacgcgc agcgcgcaga cgcgcccgcc ggcggcgttg
    15001 gccggccccg cgaacacctt gaattccacg tccttcaccg cgtcggtgat ttcggccagc
    15061 ttgagcgcaa tgcgcaggtc gggcttgtcc gagccgtagt cgcgcatcgc ttcgtcatag
    15121 gtcagcaccg ggaagggcgc cggcagctcg acgccgatcg tctcgcggaa cacgaagcgc
    15181 accagcgact ccatcagggc gcggatgtcg ttttccgaca ggaaagacgt ctcgatgtcg
    15241 atctgggtga actcgggctg gcggtcggcg cgcaggtcct cgtcgcggaa gcacttgacg
    15301 atctggtagt agcggtcgaa cccggccacc atcagcatct gcttgaagag ctgcggcgac
    15361 tgcggcagcg cgtagaaatg cccttcgtga agccgcgagg gcaccaggaa ttcgcgcgcg
    15421 ccttcgggcg tcgacttgta gagcaccggg gtctcgatgt cgacgaagcc gtgctcgtcg
    15481 agaaagcgcc gcaccgccat cgccgcgcgg tgccgcaggc gcaggttgcg ctgcatcggc
    15541 gcgcgacgca ggtcgaggat gcgatgctgc aagcgggttt cttcggaaag gttttcctcg
    15601 tcgatctgga acggcggcgg cgcggaaggg ttgaggatct cgacctcgtg cgcgagcacc
    15661 tcgacctcgc ccgtggggag ctcggggttc acggtgcccg cggggcggcg ccgcaccttc
    15721 ccggtcactc ttaagacgta ctcggagcgc acgcgctcgg cgatgcggaa ggcttcctgg
    15781 cgatcgggat cgcacaccac ctgcgccagc ccctcgcgat cgcgcaggtc gatgaagatc
    15841 acgccgccgt ggtcgcgccg ccggtgcgcc cagccgcaca gcgtcaccgt gcgatcgacc
    15901 agggtcgccc gcatttcccc gcagtaatgg ctgcgcatta tttttgcggg ggcaccacgc
    15961 ccatggaaat gatgtatttc agggcctggt cgacgctcat gtcgagctcg atcacgttct
    16021 tgcgcgcgat gatgacgaag tagccgccgg tcgggttcgg cgtggtgggc acgtacaccg
    16081 cgacgcagtc gggcggcagg tggttcgcca cgccgccgcc gggcgtgccg gtgagaaacg
    16141 cgatcgtcca catcccttcg tgcggccact gcaccaggag ggccttgcgg aacgcctggc
    16201 cgctcgacga gaacagcgta tcggagatct gcttgacgct cgagtagatc gagttcacca
    16261 ccgggatgcg gtgcaggatc tcgtgccaga gctcgaccag ctgcgcgccg aagaagttgg
    16321 tggcgaacac gccggtgagg aacacgatca ggaccgtgag gatggcgccc agcccgggaa
    16381 tgtgcacccc gagccaattc tcggtatgca gcgccgccgg cagcaagtgc agcgactggt
    16441 cgaggacatc gaccacgagc ttgaggaccc agatggtgat ggccagcggg atccagatca
    16501 gcaggccggt aatgaagtac ttcttcatcg gacgtatttc ttcatcggaa acaaa
//
LOCUS       DATBSZ010000191         1754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_510169, whole genome shotgun sequence.
ACCESSION   DATBSZ010000191 DATBSZ010000000
VERSION     DATBSZ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510169"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="VIG70_12285"
     CDS             complement(<1..1382)
                     /locus_tag="VIG70_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8251375.1"
                     /translation="MATPADPNPKKGINEEQQQELDQAFARARKALAVIETYDQARVD
                     RLCQAVAWAVANKRTFTRLVEMGIQESGLGDPVSRMGKRMKIRGVLRDALRQRSVGVI
                     EELPEKGIVKYGKPAGVIACIVPTTNPDLTPAGNAIYAIKARDVVIYSPHPRSKKTSF
                     ETVRLMREALEREGAPADILQCLTKVNIPMSQALMARADLIIATGGQPMVRAAYSSGT
                     PAYGVGAGNSTMIIDETANIEEAARNTKLSKTSDFGSGCSADGNLIIDERIFEPLLAQ
                     LQKEGGYLASAQEKEALRRVMWDGEAHRTPDTVAIAPQQLAKAAGFTIPADRAFIIVH
                     GDGIGREHPFSGEKLTTLLAVYKYKGFDQALAMMRGVYEVGGKGHSCGIYSFDDDHIH
                     RLALAAPVSRIMVRQPQSKANAGAFNNGMPMTSSLGCGTWGGNVVSENVHLKHYLNTT
                     WVSVPIKEDKP"
BASE COUNT          234 a          603 c          604 g          313 t
ORIGIN      
        1 ggcttgtcct ccttgatcgg caccgacacc cacgtggtgt tcaggtagtg cttgaggtgc
       61 acgttctccg agacgacgtt gccgccccag gtgccgcagc ccaggctgga ggtcatcggc
      121 atgccgttgt tgaacgcgcc ggcattggcc ttcgactgcg gctggcggac catgatccgc
      181 gacaccggcg cggcgagcgc gaggcgatgg atgtggtcgt cgtcgaacga gtagatcccg
      241 caggaatgac ccttgccgcc gacctcgtag acgccgcgca tcatcgcgag cgcctggtcg
      301 aagcccttgt acttgtagac ggcgagcagc gtcgtgagct tctcgcccga gaacggatgc
      361 tcgcgcccga tgccgtcgcc gtgcacgatg atgaacgcgc ggtcggcggg gatcgtgaac
      421 ccggccgcct tggcgagctg ctgcggggcg atcgccacgg tgtcgggcgt gcggtgcgcc
      481 tcgccgtccc acatcacgcg gcgcagcgcc tccttctcct gcgcgctcgc caggtagccg
      541 ccttccttct gcagctgggc gagcagcggc tcgaagatgc gctcgtcgat gatcaggttg
      601 ccgtcggccg agcagccgga gccgaagtcc gacgtcttcg acagcttggt gttgcgcgcc
      661 gcttcctcga tgttcgcggt ctcgtcgatg atcatcgtcg agttgccggc gcccacgccg
      721 tacgccggcg tgcccgagct gtacgccgcg cgcaccatgg gctggccccc ggtggcgatg
      781 atcaggtccg cgcgcgccat caacgcctgc gacatcggga tgttcacctt cgtcaggcac
      841 tgcaggatgt ccgccggcgc gccttcgcgt tcgagcgcct cgcgcatcag gcgcacggtc
      901 tcgaacgagg tcttcttcga gcgcgggtgc ggcgagtaga tgaccacgtc gcgggccttg
      961 atcgcataga tcgcgttgcc ggcgggcgtg aggtccgggt tggtcgtcgg cacgatgcag
     1021 gcgatgacgc cggccggctt gccgtacttc acgatgccct tctccggcag ctcctcgatc
     1081 acgccgacgc tccgctgccg gagcgcgtcg cgcagcacgc cgcggatctt catgcgcttg
     1141 cccatgcggc tcaccggatc gccgagcccg gattcctgga tccccatctc gaccaggcgc
     1201 gtgaacgtcc gcttgttcgc gacggcccag gcgaccgcct ggcagagccg atcgacgcgc
     1261 gcctggtcgt acgtctcgat gaccgccagc gccttgcgcg cgcgggcaaa tgcctggtcc
     1321 agctcctgtt gctgctcctc gttgatgccc tttttcggat tgggatcggc aggggtggcc
     1381 atggcgtagt cctcggcgtg ggtcggcgcg gattgggaat ctgggaaggc gtgcgtacgg
     1441 tgcagccgcg aatcgtggcg cgcgcgtggg tcaggtcgcc gcggtgaacg gtcgcctgcc
     1501 cgacatggag aggacgatag gatcgcgccc gacctccggt caagggcgca tggcgacaaa
     1561 ccgtgcgcta tgcgccccca aaatgcgccg gcggccgttt cgccgggtat cctccggcgt
     1621 atcgtgtcgg gcgtttcccg gcctcctacg accccgatgc ccgcccgcga tctgaacgcc
     1681 acgctggatg cggcggacgt gcgtccgtcg gccggcccgc cgtcggccac cgaccatcac
     1741 gcgctgccgg cgga
//
LOCUS       DATBSZ010000192         2900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1808072, whole genome shotgun sequence.
ACCESSION   DATBSZ010000192 DATBSZ010000000
VERSION     DATBSZ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808072"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VIG70_12290"
     CDS             <1..1050
                     /locus_tag="VIG70_12290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY8251376.1"
                     /translation="GAAALPRRRQRPAVVRRLLIAHHLLLGDTIMLTPLIKKARERFP
                     QTEIVMTCPRAYAPIYAGRPYGLTVLPFDPRSLSDHRALRRQRGFDLALVPGDNRWSA
                     LARALDARWIVAFAPDERSYKDWPIDEMRAMPDTPMAWGDIAARLVDGDDPQPYSPAE
                     WPAPPCKKYQLPKKPYCVLHLGASSPHKLWPSERWREVRDWAEARDYEVVLSAGRGEE
                     DLLAPVDPERTRSALAGRLDLAQLWDLLRNAAFLVCPDTGVAHLARLVGVPTVALYGP
                     GSPVSTGPGLFWAKSPFRAVWDPDVACRDQNRLFERRLVWLRQCWRSVEECGNPVCIR
                     RVAVRDVIAAIEELT"
     gene            1047..1793
                     /locus_tag="VIG70_12295"
     CDS             1047..1793
                     /locus_tag="VIG70_12295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8251377.1"
                     /translation="MSDLEQYFSRNTGRQIHKWMHYFEIYERHFSRFRGTRVHLVEFG
                     VDQGGSLQMWKHYFGPDCRIFGVDINPQCRQAEEERIEILIGDQGDRQFLRSLKTRIP
                     QIDILIDDGGHTMKQQIATYEELFPHVDANGVYVCEDLHTSYWPDWGGGLQKRGTFVE
                     YSKRFIDELHGWHFRRRGRAAITALTRTAHSLHYYDSVLVIEKRPIEQPRRQQTGTAA
                     IPQWSPPESAVKRFRKEMKRKAKRWLGRST"
     gene            complement(1807..2574)
                     /locus_tag="VIG70_12300"
     CDS             complement(1807..2574)
                     /locus_tag="VIG70_12300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8251378.1"
                     /translation="MSPAPRAPLSAVLITRNAAAVLEPCLDSLAFADEVVIVDSSSSD
                     GTVELASRKGARVVPKEWLGFGRQKQFAVEQASHDWVLCLDADERVSPRLARSIERAL
                     AAPVSPVYRMPRRNRFLGRWLSHGEGYPDWSPRLFNRMNARWSDDLVHEKVLFAVTPG
                     TLEGDLMHDSFDDLSAYLERQNRYTTLAARQAFEQGRSANVLYLLFSPVVRFIKFYIM
                     RLGFLDGLPGLLHISIGCINSYVKYMKLIELRQSENK"
     gene            2616..>2900
                     /locus_tag="VIG70_12305"
     CDS             2616..>2900
                     /locus_tag="VIG70_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEY8251379.1"
                     /translation="MTTILFVKTSSLGDVVHNCPAVSDAARALPGVAIDWIVEEPFAG
                     IAAMHGAVRRVIPVAVRRWRSGLWKPEVWAEIGAWRREVRRSRYDAVVDTQ"
BASE COUNT          478 a          968 c          992 g          462 t
ORIGIN      
        1 ggcgcggcgg cgttgccgcg gcgccgccag cgccctgcgg tcgtgcgccg gctgctcatc
       61 gcccaccact tgctcctcgg cgacacgatc atgctgaccc cgctcatcaa gaaggcgcgc
      121 gagcgctttc cgcagaccga gatcgtgatg acctgcccac gggcgtacgc gcccatctac
      181 gccggcaggc cctacggcct caccgttctg ccgttcgacc cgcgctcgct gagcgaccac
      241 cgtgcccttc gccggcagcg cggcttcgac ctcgcgctcg tccccggcga caaccgctgg
      301 tcggcgctcg cccgggcgct cgatgcgcgc tggatcgtcg ccttcgctcc cgatgagcgc
      361 agctacaagg attggccgat cgacgagatg cgggccatgc cggacacgcc gatggcctgg
      421 ggcgacattg ccgcgcgcct ggtcgacggc gacgatccgc agccgtactc gcccgcggaa
      481 tggcccgcgc caccttgtaa aaaatatcaa cttccgaaaa aaccgtactg cgtgctgcat
      541 ctcggggcga gcagcccgca caagctctgg ccgagcgaac gctggcggga agtgcgcgac
      601 tgggccgagg cgcgcgacta tgaggtcgtg ctgagcgcgg gccgcggcga ggaagacctg
      661 ctcgcgccgg tggatccgga gcgtacacgc agcgcgctcg ccggccgcct ggatctggcg
      721 cagctttggg acctcctgcg caacgccgct ttcctcgtgt gccccgatac cggcgtcgcc
      781 catcttgccc gcctggtcgg cgtgccgacg gtggcgctct acgggcccgg ctcgccggtg
      841 agcaccggcc cgggcctgtt ctgggcgaag agcccgtttc gggcggtatg ggaccccgac
      901 gtggcgtgcc gcgaccagaa tcgcctcttc gagcggcgct tggtctggct gcgccagtgc
      961 tggcgcagcg tcgaggagtg cggcaacccg gtgtgcatcc ggcgggtggc ggtgcgcgac
     1021 gtaatcgcgg cgatcgagga gctgacgtga gcgacctgga gcagtacttc tcccggaaca
     1081 cgggccggca gatccacaag tggatgcact acttcgagat ctacgagcgg cacttctcgc
     1141 ggtttcgcgg cacccgcgtc catctggtcg agttcggcgt cgaccagggc ggctcgctgc
     1201 agatgtggaa gcactacttc gggcccgatt gccggatctt cggcgtcgac atcaatccgc
     1261 aatgccggca ggcggaggag gagcgcatcg agatcctgat cggcgatcag ggggaccggc
     1321 agttcctgag gtctctcaag acccgcattc cgcagatcga catcctgatc gatgacggcg
     1381 gccacaccat gaagcagcag atcgccacgt acgaggagct gttccctcac gtcgacgcca
     1441 atggcgtcta tgtctgcgag gatctgcaca cgtcgtactg gccggactgg ggcggcggct
     1501 tgcagaagcg cggcacgttc gtcgagtaca gcaagcgctt catcgacgag ctgcacgggt
     1561 ggcacttccg gcgccgcgga cgcgccgcca tcaccgcgct caccaggacg gcgcactcgc
     1621 tgcactacta cgacagcgtc ttggtgatcg agaaacggcc catcgagcag ccccgccgcc
     1681 agcaaaccgg cacggccgcg atcccgcagt ggtctcctcc cgagagcgcc gtcaagcgct
     1741 tcaggaagga aatgaagcgc aaggcgaagc gctggctagg gcgctcgacc tgaggtagta
     1801 ggagcgttac ttgttctcgg actggcgcag ctcgatcagc ttcatgtact tcacgtagct
     1861 gtttatgcag ccgatcgaga tgtgcagcag gccgggcagg ccgtcgagga agccgagccg
     1921 catgatgtag aacttgatga agcgcacgac cggagaaaac aggaggtaca gcacgttcgc
     1981 gctgcgcccc tgctcgaacg cctgccgagc ggcgagcgtg gtgtagcggt tctggcgctc
     2041 gagataggcc gaaagatcgt cgaacgaatc gtgcatcagg tcgccctcga gggtgcccgg
     2101 cgtcaccgcg aacagcacct tctcgtgcac caggtcgtcg ctccagcgcg cgttcatgcg
     2161 gttgaagagc cgcgggctcc agtcggggta gccctcgccg tgcgacagcc agcggccgag
     2221 aaaacggttg cgtctcggca tgcggtacac gggcgacacc ggtgcggcga gcgcccgctc
     2281 gatcgaccgc gcgagccgcg gcgacacccg ctcgtcggcg tccaggcata gcacccagtc
     2341 gtggctcgcc tgctcgaccg cgaactgctt ctgccggccg aagccaagcc actccttcgg
     2401 caccacgcgc gcccccttgc ggctcgcaag ctcgaccgtg ccgtcgctcg aggacgagtc
     2461 gacgatcacg acttcgtcgg caaacgcgag gctgtcgagg cagggctcga gaacggcggc
     2521 ggcgttgcgg gtgatgagga ctgccgagag cggcgctcgt ggcgcggggc tcaaattcgc
     2581 tattccttta gaatcagcga gccgtattct agccaatgac aacaatactc ttcgtcaaga
     2641 cgtcctctct tggcgatgtg gtgcataact gtccggcagt gagcgacgcg gcgcgggcgc
     2701 tgccgggcgt agccatcgac tggatcgtgg aagagccctt tgccggcatc gccgcgatgc
     2761 acggggcggt gcggcgcgtg atcccggtcg ccgtgcggcg ctggcgctcg gggctgtgga
     2821 agcccgaggt gtgggccgag atcggggcgt ggcggcggga ggtccgccgc agccgctacg
     2881 acgcggtggt cgacacgcaa
//
LOCUS       DATBSZ010000193        12882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1330257, whole genome shotgun sequence.
ACCESSION   DATBSZ010000193 DATBSZ010000000
VERSION     DATBSZ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330257"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="VIG70_12310"
     CDS             <1..1228
                     /locus_tag="VIG70_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY8251380.1"
                     /translation="RRYWQPVALVDEMQGERPVKAVRILGEDFVLFRNGKQFGLIDRH
                     CAHRGADLAYGRLEDGGLRCSFHGWLYDASGKCLETPAEPEGSRLCEHIRLRACPVVE
                     RSGVLFAYLGAGEPPAFPHFDCFVAPPEYTFAFKGYWDCNWLQALEVGIDPAHASWLH
                     KFFEDEDPAASYGKQFRGVPSDSALPISKVLREYDRPEIRVERTGYGMRLHTLRRISD
                     AHTHIRVTNILFPQAFVIPMNAEMTISQWHVPVDDTGCYWYSIFTSFTTPVDKDTMRS
                     QRLKTYPAPHYKPIFGRHNGWGFDAEEQKHRTFTGMGFDINIHDQWACESQGRIQDRT
                     RENLGSTDKGIVLYRRLLLDAIRKNETGEQPMMVSDADQAVGPPAIDGIGPTASIDQY
                     WKESDETRRKRSAWAA"
     gene            1225..2580
                     /locus_tag="VIG70_12315"
     CDS             1225..2580
                     /locus_tag="VIG70_12315"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY8251381.1"
                     /translation="MNLPKGIKAVRFSFADQHGVLRGKTLAASAAEAAMGRGVTVTST
                     LLLKDTSHKTVFPAFTPGGGVGLLEMQGAADILMVPDESTFRVLPWAPDTGWFLCDLQ
                     FLDGREVPFSTRGQLRKCLERLESAGYEFVAGLEVEFHVFKLRDPRLQPEDAGQPGTP
                     PDVELLTTGYQLLTEHKYDQLDPVVEIFRTNLEQLELPLRSFEIEFGPSQLEFTLAPR
                     TGISAADAMILLRSAVKQIARRHGYHATFMCRPKLPNVMSSGWHLHQSLRRNGLNAFT
                     SDRDDLSETGKQYLAGLLAHGRAAAAFATPTINGYKRYRPYSLAPDRVIWGKENRGAM
                     LRVVGAPRDPDTRIENRIGEPAANPYLYFASQIYCGMDGVERRLPLARAADTPYEAKA
                     DFLPRTLEEALQHLRIDKALRDGFGAGFVDYYCRIKEAEIARFNLEVSDWEHREYFDL
                     F"
     gene            2594..3577
                     /locus_tag="VIG70_12320"
     CDS             2594..3577
                     /locus_tag="VIG70_12320"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8251382.1"
                     /translation="MDQRELGRSGMKISALGLGLMSMSGVYGNANDEESIGVIHYALD
                     QGINFLDSADMYGWGHNETLLGKALKGRRDKAIVATKFGQVKQADGRQTVDGRPEYVM
                     QACDASLKRLGIDVIDLYYQHRVDANTPIEDTVGAMKRLVEQGKVRALGLSEARPETI
                     RRAHKVHPIAAVQNEYSLLYRNEGEETRQATRELGITLVPYAPLGRSMLTGTVHGKAD
                     LPEGDRRLQHPRFQGDALDKNVALVKRIEEIAREKRCTPAQLVLAWLLAQGKDMVPIP
                     GTKRRERIDENLAALKIRLTAEDLKRISDAAPPGAGAGTRYPAETMKRVYL"
     gene            3613..4431
                     /locus_tag="VIG70_12325"
     CDS             3613..4431
                     /locus_tag="VIG70_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8251383.1"
                     /translation="MVWSLQYIFLRVAVPVFGTATVAEGRALFAALFLLPWVAWVAHQ
                     RIGLLEHWKDHLAVGLVNSVLPFVCFAWAATVLPAGYLAIINGTVPLWTAVIAAPVLK
                     ERLGPGRIAGFALGIAGIALIVNLGPAELSLRTVLAVLVSLAGAGSWAWAGVMIKERS
                     GRLPPVSLAAGSIALSALILAPLWAAAPPPQEWTLPAFASLVVLGAVCSGLAYLPFFT
                     LVRDIGPSRTLTVGFVVPVLGVLWGWLFLDEAVTLGMLAGTTLVLAALTLVLRR"
     gene            4493..5932
                     /locus_tag="VIG70_12330"
     CDS             4493..5932
                     /locus_tag="VIG70_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEY8251384.1"
                     /translation="MPMMKKVTLAVALALSPVAYGAVTEKPSTLADQQSVAVTIYNEN
                     LALVKDQRRVAFESGRNRLALREVSGRMRPETALLRSLSHPGALTLLEQNFDFDLLTP
                     AKLLEKYVGRDVRIVRTHPTTGAESIETATVLAATSGVVLRIGDRIETGLPGRIVYDG
                     VPANLRDRPTLVTELDAARAGSQAIELSYLTGGLAWKADYVAELNGADTALDLNGWVT
                     LTNTSGTAYPNAKLQLVAGDVNRVRDEMRLAAKAGAMRESAAAPASQMSQEALFEYHL
                     YTLGRPTTIADNQTKQVALLNAQRVPVAKELVLQGSDYYYRSSVGGIGQKMKVGVFVQ
                     LENRESSHLGMPMPKGVVRVYKKDSAGNAQFVGEDRIDHTPKNETVRLKLGEAFDVTA
                     DKKQTDFKRREPTNRASYVFESAYEIVLRNAKKEAATIVVREPVPGDWSMLEETERHA
                     KVAAGAAEWRVRVPAEGSTTLRYRVLVRY"
     gene            complement(5934..6518)
                     /locus_tag="VIG70_12335"
     CDS             complement(5934..6518)
                     /locus_tag="VIG70_12335"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY8251385.1"
                     /translation="MDTPPSGFLKRLAAGCALVATAALAHAQALDRPMLLIAPPTADG
                     IYSQAVLVVVPIHEGQHRGFIINRVSHTTVARAFPDEPDMAKVVDPIYFGGPNDAQRM
                     YAVVRRDPGEGARKLFDDVFVTISGATVDRVIQQWPGEARFFAGYAAWEPGELAQEIR
                     DGEWLVAEPHAAQIFSLQPGDLWLQLLQRIRSTL"
     gene            complement(6723..7115)
                     /locus_tag="VIG70_12340"
     CDS             complement(6723..7115)
                     /locus_tag="VIG70_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251386.1"
                     /translation="MDTPPSGFLKRLAAGCLLVAAAATAQAQTPAWTALDYSLATGAA
                     TMMALDWAQTRYAVRHPSRYSERNPIMGEHPSTTKVDQYFALASLLMVGVTYVLPRPE
                     RRLFLGGVLVVETSAVVSNYRIGVKLDF"
     gene            complement(7225..7836)
                     /locus_tag="VIG70_12345"
     CDS             complement(7225..7836)
                     /locus_tag="VIG70_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HEY8251387.1"
                     /translation="MSEFQQLVIAGWTGRDEAALRKHIKELEEIGVKAPKTTPIFYRV
                     SANLFTHETAIQVSGLDTSGEVEFVLMQGKELRVAVGSDHTDRKAETIGVSLSKQLCA
                     KPVSRESWRYDEVKPHWETLVLRSWADGELYQEGPVTAMRSPEDLLQRYGGLKNGWAM
                     FCGTLAAKGGIRPAGRFAMELEDPVLKRRLKHEYRIHALPIEG"
     gene            complement(7833..9305)
                     /locus_tag="VIG70_12350"
     CDS             complement(7833..9305)
                     /locus_tag="VIG70_12350"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8251388.1"
                     /translation="MLKTGKAHLESLRDGRVVYIGSEKVDDVTTHPAFRNGARSMAAI
                     YDLKAANPDFSYEENGERFSAYFLRAKTKDDLVQRTELHRAIARMSHGLLGRSPDHVS
                     SFVTGMAMNPEVFKGFRKNLLDYYEHMRREDVYGVYAVLPPQAARNPEFYVKQNLPVP
                     SLRVVKEDDAGVTITGMKMLATGAVFANELWIGNLLPLAPNQLAESITCALPVNAPGV
                     TLWSRKPFERYAPSEFENPLTWRFDETDSMVMCENVKVPWERVFVHMDAILAREIYIK
                     TPGHCYGNHQSNVRFHAKMQLIVGLASKIAHASGANEVPAVREVLGRFAALEAALGGM
                     IAGQLHEAEDWPEGWKTFNRRMMYAALNWCTESHSSIIDALRELIGGGIFQMPADITV
                     MHDAKLKQMFETYWQTPQLGAVERMKLYKLAWDLIGSEFAGRHLQYEKFYAGASFIVR
                     NHSFREAPWEGFHKVVDDLMASYDMPAKESGTDHVFRGVR"
     gene            complement(9289..9675)
                     /locus_tag="VIG70_12355"
     CDS             complement(9289..9675)
                     /locus_tag="VIG70_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEY8251389.1"
                     /translation="MAVKPKQHLEFVKVDLKSGWEVPPGYPAGFYQKILASDLDEKAK
                     RGSRTRLLKIEPGAFSTEPFVHDHWEEVYVVQGDLVVGNDKQGKGGQQCFAPTYACRP
                     PGVHHGPFTSRGGCILHEIHYYAEDR"
     gene            complement(9666..10001)
                     /locus_tag="VIG70_12360"
     CDS             complement(9666..10001)
                     /locus_tag="VIG70_12360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEY8251390.1"
                     /translation="MSVSYFVRYDIAVADLEAFLERYRRDHVPMVTAWPGLQRMTLHT
                     PVDWKDPFPVNRGRAVLMAQLEFESEEAMNRAFASRERAEAREDFKRFMTFEGTVTHQ
                     AMQSEEVWP"
     gene            complement(9998..10546)
                     /locus_tag="VIG70_12365"
     CDS             complement(9998..10546)
                     /locus_tag="VIG70_12365"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8251391.1"
                     /translation="MARARRQTPPRNPPPTVSRPALLARGSDAEFRGLIHDLIAYGHK
                     LDACRDAFAAIAGVSGVQYEILMLVSRADGLAVGEVAARLHRSGAFITIEANKLAGRG
                     ILDKASDPADGRRVLLKNTSRSFALLERLAPYQRRVNDVLFEFLDAKRFRELRALAAQ
                     LVGSGDRAVAMLELLRHEAQAA"
     gene            complement(10587..>12882)
                     /locus_tag="VIG70_12370"
     CDS             complement(10587..>12882)
                     /locus_tag="VIG70_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY8251392.1"
                     /translation="TVPGAQSTNEDTQLVLTGATRLNVADVDATSLTVNLSVTAGTLT
                     LGGIAGLSFSAGDGTADGAMIFNGTIASIQTALASVTYNPAINSTAAATLTLTLSDNG
                     QTGAGGALTDTDNLTISINAVNDAPDAQDDTYTVLEDQLLAVAAPGVLGNDSDVDSVL
                     TAAVMDDVDHGTLSLAADGSFLYAPDLNFFGTDTFIYEVNDGQGGTDTATVTITVVAD
                     NDGPVAVDDSATTAEDVAVSIAVLANDTDVENDLLFVTDVGLASHGTVTFSAGAVIYT
                     PNLNFHGTDTFEYTVSDGEEIDTGRVTVTVTPVNDPPVAIDDVATTDEDVAVTISVLA
                     NDQDVDGDSLNVGSVTQGANGTVTFSAGAVTYTPDAGFSGTDFFDYTASDGQGGSDVG
                     RVTVTVDAADDNDAPVFVEVADRTITEGQELRFTVSAIDPDHPESSLVFSATSLPAGA
                     TFNAMTRELVWIPADNAIATVTFTVLDPEGATDTMEVVLTATNVAATVNAGADQVVGL
                     QKKHGHDHDDDDHHGHGHGHGHKKGEATVSISATFNDLGTLDTHANRYTTIDWGDGDV
                     TEGAVSESPFGPPGSTAGANGTVTGTHTYKETGVYTVKVTVTDDDGASRSDTLTVTVK
                     DPDKHLKARDDEYQLREDGVLQVNAAHGVLRNDRAPGSLPLTARLVEGPDHGELTFNA
                     DGSFTYRPDANFHGKDSFWYEFTDGTNVSNAVKVEFNVKSDGRREACIDWGGRPGWDL
                     PHFGKHANFAEFLVKLARKFDLES"
BASE COUNT         2098 a         4361 c         4228 g         2195 t
ORIGIN      
        1 gcggcgctac tggcagccgg tcgccctggt ggacgagatg cagggcgagc ggccggtgaa
       61 agcagttcgc atcctcggcg aagattttgt tttattcaga aatggaaagc agtttgggtt
      121 gatcgaccgc cactgcgcgc atcgcggcgc ggacctcgcc tacgggcgtc tcgaagacgg
      181 cgggctgcgc tgctcgttcc acggctggct ctacgacgcg agcggaaaat gcctcgagac
      241 gccggccgag ccggagggca gccgcctgtg cgagcacatc cgcctgcgcg cctgcccggt
      301 ggtggagagg agcggcgtgc tcttcgccta cctcggcgct ggcgagccgc ccgcgtttcc
      361 gcacttcgac tgcttcgtcg cgccgcccga gtacacgttc gcgttcaagg gctactggga
      421 ctgcaactgg ctgcaggcgc tcgaggtcgg catcgacccg gcgcacgcct cgtggctgca
      481 caagttcttc gaggacgagg accccgcggc gagctacggc aagcagttcc gcggcgtgcc
      541 gtcggattcg gcgctgccga tctcgaaagt gctgcgcgag tacgaccggc cggagatccg
      601 cgtcgagcgc accggctacg gcatgcggct gcacacgctg cgcaggatca gcgatgcgca
      661 cacccacatc cgggtgacga acatcctctt tccgcaggcc ttcgtgatcc cgatgaatgc
      721 cgagatgacg atctcgcagt ggcacgtgcc ggtcgacgac acgggctgct actggtactc
      781 gatctttacc agcttcacga ccccggtgga caaggacacg atgcgcagcc agcgcctgaa
      841 aacctacccg gcgccgcact acaagccgat ttttggccgc cacaacggct ggggcttcga
      901 cgcggaggag cagaagcacc gcaccttcac cggcatgggg ttcgacatca acatccacga
      961 ccagtgggcg tgcgagtcgc agggacgcat ccaggaccgc acgcgcgaga acctcggcag
     1021 caccgacaag ggcatcgttc tctatcgccg gctgctcctg gatgcaatcc ggaaaaatga
     1081 aactggggaa cagcccatga tggtttctga tgctgaccaa gcggtgggcc cgccggcgat
     1141 cgacggcatc gggccgaccg ccagcatcga ccagtactgg aaggagtccg acgaaacccg
     1201 gcgcaagcgc tcggcctggg cggcatgaac cttccgaagg ggatcaaggc ggtccgcttt
     1261 tcgttcgccg accagcacgg cgtgctgcgc ggcaagacgc tcgccgcgtc tgcggccgag
     1321 gcggcgatgg ggcgcggcgt cacggtcacg agcacgctgc tcctcaagga cacctcgcac
     1381 aagacggtgt ttccggcctt cacaccgggc ggcggcgtcg gcctgctcga gatgcagggc
     1441 gcggccgaca tcctgatggt ccccgacgag tcaaccttca gggtcctgcc ctgggcgccg
     1501 gacaccggct ggttcctgtg cgacctgcag ttcctcgacg gccgcgaggt gccgttcagc
     1561 acccgggggc aactccgcaa gtgcctggag cggctggaga gcgcggggta cgaattcgtc
     1621 gccggcctgg aagtggaatt ccacgtcttc aagctgcgcg atccgcgcct gcagccggaa
     1681 gatgccggtc agccgggcac gccgccggac gtggagcttc tcaccaccgg ctatcagctc
     1741 ctcaccgagc ataaatacga ccagctggac cccgtggtgg agattttcag gaccaatctc
     1801 gagcagctcg agcttccact gcgttcgttc gagatcgagt tcggcccgag ccagctcgag
     1861 ttcacgctcg cgccgcggac cggcatttcg gcggcggacg cgatgatcct gctgcgcagc
     1921 gcggtgaagc agatcgcgcg gcgccacggc taccacgcca ccttcatgtg ccggccgaag
     1981 ctgccgaacg tcatgtcgag cggctggcat ctgcaccagt cgctgcgaag aaacgggctg
     2041 aacgcgttca cctccgaccg cgacgatctc tccgagaccg gaaagcaata cctcgccggg
     2101 ctgctggcgc atggtcgcgc ggcggccgcg ttcgccacgc cgacgatcaa cggctacaag
     2161 cgctatcgcc cctactcgct cgcgcccgac cgcgtcatct ggggcaagga gaaccgcggc
     2221 gcgatgctgc gcgtggtcgg cgcgccgcgc gatccggaca cccgcatcga gaaccgcatc
     2281 ggcgagccgg cggcgaatcc gtacctctac ttcgcctcgc agatctactg cggcatggac
     2341 ggcgtcgagc ggcgcctgcc gctcgcgcgc gcggccgaca cgccgtacga agcgaaggcc
     2401 gatttcctgc cgcgcacgct cgaggaagcg ctgcagcacc tgcgcatcga caaggcgctg
     2461 cgcgacggct tcggcgccgg ctttgtcgac tattactgca ggatcaagga agccgagatc
     2521 gcgcgcttca acctcgaggt gagcgactgg gagcaccggg aatatttcga cctgttctaa
     2581 ggagaatcaa cgcatggatc aacgcgaact cggccgcagc ggaatgaaga tctccgccct
     2641 cggcctcggc ctgatgtcga tgtcgggcgt gtacggcaac gcgaacgacg aggagtcgat
     2701 cggcgtcatc cactacgcgc tcgaccaggg catcaatttc ctcgactcgg ccgacatgta
     2761 cggctggggg cacaacgaga cgctgctcgg gaaggcgctc aagggacggc gcgacaaggc
     2821 gatcgtcgcg acgaaattcg gccaggtgaa gcaggccgac ggaagacaga ccgtcgacgg
     2881 ccggccggaa tacgtgatgc aggcgtgcga cgcgagcctc aagcgcctcg gcatagacgt
     2941 gatcgacctc tactaccagc accgcgtcga tgccaatacg ccgatcgagg acaccgtcgg
     3001 cgcgatgaag cggctcgtcg agcagggcaa ggtgcgcgcg ctcgggctgt ccgaggcgcg
     3061 tcccgagacc atccggcgcg cccacaaggt gcacccgatc gcggcggtgc agaacgaata
     3121 ctccctgctc taccggaacg agggcgagga aacgcggcag gcgacgcgcg agctcggcat
     3181 cacgctcgtc ccctacgcgc cgctcggccg aagcatgctc accggcacgg ttcacggcaa
     3241 ggcggacctt ccggaaggcg accgccgcct gcagcatccg cgcttccagg gcgatgcact
     3301 ggacaagaat gtcgccctcg taaagcgcat cgaggaaatc gcccgggaaa agcgctgcac
     3361 gcctgcgcag ctcgtgctgg cgtggctcct cgcgcaggga aaggacatgg tgccgatccc
     3421 cggcaccaag cggcgcgagc gcatcgacga gaacctcgcg gcactgaaaa tcaggctgac
     3481 ggccgaagat ctgaagcgca tctccgatgc cgcgcctccc ggagcaggag ccggaacgcg
     3541 ctatcccgca gagaccatga aacgcgtgta cctctgacgg ctcaccagac cacacggtgg
     3601 ctcaccctcg ccatcgtgtg gtcgctgcag tacatcttcc tgcgcgtggc ggtgccggtg
     3661 ttcggcacgg cgacggtcgc cgaaggccgc gcgctcttcg ccgcgctgtt cctcctgccc
     3721 tgggtcgcct gggtcgcgca ccagcgcatc ggcctgctcg agcactggaa ggatcacctc
     3781 gcggtcgggc tggtgaacag cgtgctgccg ttcgtctgct tcgcgtgggc ggccacggtg
     3841 ctgccggcgg gctacctcgc gatcatcaac ggcaccgtgc cgctctggac cgccgtgatc
     3901 gccgcgccgg tgctgaagga gcggctggga ccggggcgca tcgccggctt cgcgctcggc
     3961 atcgccggca tcgcgctcat cgtgaacctg ggcccggcgg agctcagcct gcgcaccgtg
     4021 ctcgcggtgc tcgtgagcct cgccggcgcg ggctcctggg cctgggcggg cgtcatgatc
     4081 aaggagcgct cggggcggct gccgccggtc agcctcgccg cgggctcgat cgcgctcagc
     4141 gccctgatcc tggcgccgct ctgggccgcc gctccgccac cccaggaatg gactctgccg
     4201 gccttcgctt cgctggtcgt cctcggcgcg gtatgcagcg gcctcgccta cctgccgttc
     4261 ttcaccctgg tccgcgacat cggcccgtcg cgcacgctga ccgtcggctt cgtcgtgccg
     4321 gttctcgggg tcctgtgggg ctggctgttc ctcgatgagg ccgtgaccct cgggatgctg
     4381 gcgggcacca ccctggtgct cgccgcgctg accctcgttc tgcgccgtta accctgcctc
     4441 ctaacccttt tccttggccg ccccgtttaa cggggacaag gaggcaacac caatgcccat
     4501 gatgaaaaaa gtcacgctcg ctgtggcgct ggccctttcc ccggtcgcct acggcgccgt
     4561 aaccgagaaa cccagcaccc tggccgacca gcagtcggtc gcggtgacca tctacaacga
     4621 gaacctcgcg ctggtgaagg accagcggcg cgtcgccttc gagtccggcc gcaaccgcct
     4681 cgcgctgcgc gaggtgagcg ggcggatgcg ccccgagaca gcgctgctgc gcagcctgtc
     4741 gcaccccggc gcgctgacgc tgctcgagca gaacttcgat ttcgacctgc tcacgccggc
     4801 gaagctgctc gagaaatacg tcggccgcga cgtgcgcatc gtccgcaccc acccgaccac
     4861 cggcgccgag tcgatcgaga ccgcgactgt cctcgcggcc acctcaggcg tggtgctgcg
     4921 gatcggcgac cgcatcgaga ccgggctgcc cggacgcatc gtctacgacg gggtgccggc
     4981 caacctgcgc gaccgtccga cgctcgtcac cgagctcgac gccgcgcgcg ccggcagcca
     5041 ggcgatcgag ctgtcgtacc tcaccggcgg cctcgcctgg aaagccgact acgtcgccga
     5101 gctgaacggc gccgacaccg cgctcgacct gaacggctgg gtgacgctca ccaacacgag
     5161 cggcaccgcc taccccaacg caaagctgca gctcgtcgca ggcgacgtga accgcgtgcg
     5221 cgacgagatg cgcctcgcgg cgaaggccgg cgcgatgcgc gagagcgcgg ccgccccggc
     5281 ttcgcagatg tcgcaggaag ccctgttcga gtatcacctg tacaccctcg gacgcccgac
     5341 cacgatcgcc gacaaccaga ccaagcaggt ggcgctgctc aacgcgcagc gcgtgcccgt
     5401 cgccaaggag ctggtcctcc agggaagcga ctactactac cgctcgagcg tcggcgggat
     5461 cgggcagaag atgaaagtcg gcgtgttcgt gcagctcgag aaccgcgagt cctcgcacct
     5521 gggaatgccg atgccgaagg gcgtggtgcg cgtgtacaag aaggacagcg ccggcaacgc
     5581 ccagttcgtg ggcgaggaca ggatcgacca cacgccgaag aacgagaccg tgcgcctcaa
     5641 gctcggcgag gccttcgacg tcaccgccga caagaagcag accgacttca agcgccgcga
     5701 gcccaccaat cgcgcgagct acgtgttcga gagcgcctac gagatcgtgc tgcgcaacgc
     5761 gaagaaggaa gccgcgacca tagtggtgcg cgagccggta ccgggcgact ggtcgatgct
     5821 cgaagagacc gagcgccacg ccaaggtggc cgccggcgcc gcggaatggc gcgtccgggt
     5881 gcccgccgag ggcagcacca ccctgcgcta ccgcgtgctg gtgcgctact gacctacaga
     5941 gtgcttcgaa ttctttgcaa cagttgcagc cacaggtcgc cgggctgcag gctgaagatc
     6001 tgcgcggcgt gcggctcggc gacgagccac tcgccgtcgc gaatctcctg cgcgagctcg
     6061 cccggctccc aggcggcgta gccggcaaag aagcgcgcct cgcccggcca ttgctggatc
     6121 acacggtcca cggtcgcgcc gctgattgtg acgaagacgt cgtcgaacag cttgcgcgcg
     6181 ccctccccgg gatcgcgccg cacgaccgca tacatccgct gcgcgtcgtt cggcccgccg
     6241 aagtagatcg ggtcgaccac cttggccatg tccggctcgt cgggaaacgc cctcgccacg
     6301 gtggtatggc tcacgcggtt gatgatgaag ccgcggtgct ggccctcgtg aatgggcacg
     6361 acgaccagca cggcctggga gtagatgccg tccgccgtcg gcggggcgat gagcagcatc
     6421 ggccggtcga gcgcctgcgc gtgagcgagg gcggccgttg cgacgagggc gcagccggcg
     6481 gcgagccgct tgaggaaacc gcttggtggc gtgtccatgg accccacact acggccgcgc
     6541 actgcgcagc cgcaggaagt agccgcaccg caggacagac tcttgccgcc tcgactattt
     6601 tcttaccgtc ccgacgattt tttgcgatca gccctcgatc ggaagggcgt ggatactgta
     6661 ctcgtgcttc agcttgcgct tcgcggggcg gccgcgcact cgctcgggct tgccctggga
     6721 attcaaaagt ccagcttgac gccgatccgg taattggaaa ctaccgcgga agtttcgacc
     6781 acgagcaccc cgcccaggaa tagcctcctc tcgggccgcg gaaggacgta agtcacccct
     6841 accatgagga gagaggcgag cgcgaaatac tggtcgactt ttgtggtcga cgggtgctcg
     6901 cccatgatgg gattacgctc cgaatagcgg ctcggatggc gaacggcgta cctcgtctgc
     6961 gcccagtcga gcgccatcat cgtagcggct ccggtcgcta gcgagtaatc gagcgccgtc
     7021 caggcgggag tctgcgcctg cgcggtggcg gctgcagcga cgagaaggca gccggcggcg
     7081 agccgcttga ggaaaccgct tggtggcgtg tccatgaagc ccacactacg gccgcgcgct
     7141 gcgcagccgc aggaagtagc cgcaccgcag gacagactct tgccgcctcg actattttct
     7201 taccgtcccg acgatttttt gcgatcagcc ctcgatcggc agggcgtgga tgcggtactc
     7261 gtgcttcagc ctgcgcttca gcaccgggtc ttcgagctcc atggcaaaac ggcctgcggg
     7321 ccggatgccg cccttggcgg cgagcgttcc gcaaaacatg gcccagccat ttttcaagcc
     7381 tccatacctc tggagaaggt cctcggggga gcgcatcgcg gtgaccgggc cttcttggta
     7441 gagctcgccg tcggcccagg agcgcagcac gagcgtctcc cagtgcggct tcacctcgtc
     7501 gtagcgccag gattcgcgcg acacgggctt cgcgcagagc tgtttcgaga gcgacacacc
     7561 gatcgtttcg gccttgcggt cggtgtggtc ggagcccacg gcgacgcgaa gctccttgcc
     7621 ctgcatcagg acgaattcga cttcgccgct ggtgtcgagg ccagacacct gaatggcggt
     7681 ttcatgggtg aagaggttgg cggaaacccg gtagaagatc ggagtggtct tcggcgcctt
     7741 gacgccgatc tcttccagct ccttgatgtg ctttcgcagc gcagcctcgt cgcgtcccgt
     7801 ccaaccggcg atcaccagtt gctgaaactc gctcatcgca caccccggaa aacgtggtct
     7861 gtcccggatt ccttggcggg catgtcgtag ctcgccatca ggtcgtccac gaccttgtga
     7921 aagccctccc acggcgcttc gcgaaagctg tgattcctca cgatgaacga ggcgccggcg
     7981 tagaacttct cgtactgcag gtgccggccg gcgaactcgg agccgatcag gtcccaggcg
     8041 agcttgtaca gcttcattct ttccaccgcg ccgagctgcg gcgtctgcca gtaagtctcg
     8101 aacatctgct tcagcttcgc atcgtgcatc accgtgatgt cggcgggcat ctggaagatg
     8161 ccgccgccga tcagctcgcg cagcgcgtcg atgatcgacg agtggctctc ggtgcaccag
     8221 ttgagcgcgg cgtacatcat gcggcggttg aaggtcttcc agccttccgg ccagtcctcg
     8281 gcctcgtgca gctgcccggc gatcatgccg ccgagcgcgg cctcgagcgc ggcgaagcgg
     8341 cccagcacct cgcgcaccgc cggcacctcg ttggcgcccg aggcgtgcgc gatcttgctc
     8401 gccagcccga cgatgagctg catcttggcg tggaagcgca cgttcgactg gtggttgccg
     8461 tagcagtggc ccggggtctt gatgtagatc tcgcgcgcga ggatcgcgtc catgtgcacg
     8521 aacacccgct cccagggcac ctttacgttc tcgcacatca ccatcgagtc ggtctcgtcg
     8581 aagcgccagg tgagcgggtt ctcgaactcg ctcggcgcat agcgctcgaa gggcttcctc
     8641 gaccacagcg tgacgcccgg ggcgttcacc ggcagcgcgc aggtgatcga ttcggcgagc
     8701 tggttcggcg cgagcggcag caggttgccg atccagagct cgttggcgaa caccgcgccg
     8761 gtggcgagca tcttcatgcc ggtgatggtg acgccggcgt cgtcttcctt caccacgcgc
     8821 agcgacggca ccggcaggtt ctgcttcacg tagaactcgg ggttgcgcgc cgcctgcggc
     8881 ggcagcaccg cgtacacccc gtagacgtcc tcgcggcgca tgtgctcgta gtagtcgagc
     8941 aaattctttc tgaagccctt gaacacctcc ggattcatcg ccatcccggt gacgaagctc
     9001 gacacgtggt cgggcgagcg cccgagcagg ccgtggctca tccgggcgat agcgcggtgc
     9061 agctccgttc tctggacaag atcgtctttc gtcttcgcac gcaggaaata cgcgctgaat
     9121 ctttccccgt tttcttcata tgaaaaatcc gggttcgcgg ccttgaggtc gtagatcgcc
     9181 gccatggagc gcgcgccatt gcgaaacgcc gggtgcgtgg tgacgtcgtc gaccttctcc
     9241 gagccgatgt agacgacccg gccgtcgcgc agcgactcga ggtgggcctt accggtcttc
     9301 agcatagtag tgaatctcgt gcaggatgca gccgccgcgg gaggtgaagg ggccgtggtg
     9361 tacgcccggc ggccggcagg cgtaggtggg agcgaagcac tgctgcccgc ccttgccctg
     9421 cttgtcgttg ccgaccacga ggtcgccctg caccacgtac acctcctccc agtggtcgtg
     9481 cacgaaaggc tcggtagaaa acgcgcccgg ctcgatcttc agaagccgcg tgcggctgcc
     9541 ccgctttgcc ttttcatcca gatccgacgc aagaatcttc tggtagaacc ccgccgggta
     9601 tccgggcggg acttcccagc cgctcttcag gtcgactttc acgaattcga gatgctgctt
     9661 gggtttcacg gccatacctc ctcgctctgc atcgcctggt gcgtcaccgt gccctcgaag
     9721 gtcatgaagc gcttgaaatc ctcccgcgcc tctgcgcgct cgcgcgaggc gaaagcgcgg
     9781 ttcatggctt cctcgctctc gaactcgagc tgtgccatca ggaccgcgcg accgcggttc
     9841 acgggaaacg ggtccttcca gtccacgggc gtgtgaagcg tcatgcgctg caggcccggc
     9901 cacgcggtca ccatgggcac gtggtcccgg cgatagcgct cgagaaacgc ctcgaggtcg
     9961 gcgaccgcga tgtcgtagcg cacgaaatac gacacgctca tgcggcctgc gcctcgtgcc
    10021 gcaggagctc gagcatcgcc accgcgcggt cgccgctgcc caccagctgc gcggcgagag
    10081 cgcgcagctc gcggaagcgc ttcgcgtcga ggaactcgaa cagcacgtcg ttgacgcgcc
    10141 gctggtaggg ggcgaggcgt tccagcagcg cgaagcttct tgacgtattt ttcagcagaa
    10201 cccgcctgcc gtcggcggga tccgacgcct tgtcgaggat gccgcgcccg gccagcttgt
    10261 tcgcctcgat ggtgatgaac gcgcccgagc ggtgcagccg cgccgccacc tcgccgaccg
    10321 ccaggccgtc ggcgcggctc accagcatca ggatttcgta ttgcacgccg gagacgccgg
    10381 cgatcgccgc gaacgcgtcg cggcaggcgt cgagcttgtg gccgtaggcg atcaggtcat
    10441 ggatcagccc gcggaactcg gcgtcgctgc cgcgcgcgag cagcgccggg cgcgataccg
    10501 tgggcggcgg attgcgcggc ggggtctgcc ggcgggcgcg ggccatttgc cgggcgattc
    10561 tccacccggc aaattacctt tgtcaattag ctttctaagt cgaacttccg cgccagcttg
    10621 accaggaact cggcgaagtt cgcgtgcttg ccgaaatgcg gcaggtccca ccccggccgc
    10681 ccgccccagt cgatgcaggc ctcgcgccgc ccgtcgctct tcacgttgaa ctcgaccttc
    10741 accgcattcg acacgttggt gccgtcggtg aactcgtacc agaacgagtc cttgccgtgg
    10801 aagttcgcgt ccggccggta ggtgaacgag ccgtcggcgt tgaaggtcag ctcgccgtgg
    10861 tccggaccct cgaccagccg cgcggtcagc ggcaggctgc ccggcgcgcg gtcgttcctg
    10921 agcacgccgt gcgccgcatt tacctgaagc acgccgtctt ccctgagctg gtactcgtcg
    10981 tcgcgcgcct tcaggtgctt gtcgggatcc ttcaccgtga ccgtgagcgt gtcgctgcgg
    11041 ctcgcgccgt cgtcgtcggt gaccgtcacc ttgaccgtgt agacgccggt ctccttgtag
    11101 gtgtgggtgc cggttacggt accgttggcg cccgccgtcg atccgggcgg tccgaacggc
    11161 gattcgctca cggctccctc ggtgacgtca ccgtcgcccc agtcgatcgt cgtgtagcgg
    11221 ttggcgtgcg tgtcgagcgt gccgaggtcg ttgaaggtcg cggagatgct gaccgtcgct
    11281 tcgcccttct tgtggccatg gccatgaccg tgaccgtggt ggtcatcatc gtcatggtcg
    11341 tggccgtgct tcttctgcag gccgaccacc tggtccgcgc cggcattcac tgtcgccgcg
    11401 acgttggtgg cggtcagcac cacttccatg gtgtcggtgg cgccctccgg atccagcacc
    11461 gtgaaggtca cggtcgcgat cgcgttgtcc gccgggatcc agacgagctc ccgcgtcatc
    11521 gcattgaacg tggcgcccgc gggcaaggag gtggcgctga agaccaacga gctctcggga
    11581 tggtccgggt cgatggcgct gaccgtgaag cgcagctcct ggccctctgt gatcgtccgg
    11641 tccgcgacct cgacgaagac cggggcatcg ttgtcgtcgg ccgcgtcgac cgtgacggtg
    11701 acccggccga cgtcgctccc accctggccg tcgcttgccg tgtagtcgaa gaaatcggtg
    11761 ccggagaagc cggcgtctgg cgtgtaggtg accgcgccgg cgctgaacgt taccgtgccg
    11821 ttcgcgcctt gcgtaacgct gcccacgttc agggaatcgc cgtccacgtc ctggtcgttg
    11881 gccagtaccg aaatcgtcac cgccacgtcc tcatcggtcg tggccacgtc atcgatcgcc
    11941 accggcgggt cgttcaccgg cgtgaccgta accgtgaccc gtcccgtgtc gatctcttcg
    12001 ccgtcgctta ccgtgtattc gaacgtgtcg gtgccgtgaa agttcaggtt cggcgtgtag
    12061 atcaccgcgc cggcgctgaa tgtgaccgtg ccgtgcgaag cgagacccac gtcggtcacg
    12121 aagagcagat cgttctccac gtcggtatcg ttggcgagca ctgcgatgct gaccgccaca
    12181 tcctcggcag tcgtagcgct gtcatcgacc gccactgggc cgtcgttatc tgcaacgacc
    12241 gtgatcgtca cagtcgcggt gtccgtcccg ccctgtccgt cgtttacctc gtagatgaac
    12301 gtgtcggtgc cgaagaagtt gagatccggc gcgtaaagga agctgccgtc ggcggcgagg
    12361 gaaagcgtgc cgtggtcgac gtcgtccatt actgccgcgg tcagcacgct gtcgacgtcg
    12421 ctgtcgttgc ccaggacccc cggcgccgcc accgcgagca gctggtcttc cagcaccgta
    12481 taggtgtcgt cctgcgcatc cggcgcgtcg ttcacggcgt tgatcgaaat ggtgaggtta
    12541 tcggtatcgg tgagtgcgcc gccggcaccg gtctgaccgt tgtcgctaag agtgagcgtg
    12601 agtgtcgccg cagcggtgct gttgatcgcc gggttgtatg tgacgcttgc gagcgccgtc
    12661 tggatactcg cgatcgtccc gttgaagatc atcgccccgt cggccgtgcc gtcgccggcg
    12721 ctgaacgaga gcccggcaat tccgccgagc gtgagcgtcc ctgcggtcac ggacaggttc
    12781 accgtgaggc tcgtcgcgtc cacgtcggcg acgttcagcc gcgtcgctcc cgtgagcacc
    12841 aactgcgtgt cctcgttcgt gctctgcgcc ccaggtaccg tc
//
LOCUS       DATBSZ010000194         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1672353, whole genome shotgun sequence.
ACCESSION   DATBSZ010000194 DATBSZ010000000
VERSION     DATBSZ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672353"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VIG70_12375"
     CDS             complement(<1..471)
                     /locus_tag="VIG70_12375"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY8251393.1"
                     /translation="MLTFQQVILRLQEYWGSRGCALLQPYDMEVGAGTSHTATFLRAL
                     GPEPWRAAYVQPSRRPKDGRYGENPNRLFQHHQFQVVLKPSPPDILDLYLGSLEALGF
                     DLAKNDVRFVEDDWENPTLGAWGLGWEVWLNGMEITQFTYFQQVGSLDCNPITGE"
     gene            589..1611
                     /locus_tag="VIG70_12380"
     CDS             589..1611
                     /locus_tag="VIG70_12380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEY8251394.1"
                     /translation="MAESLEPVISVVVPAYNAERTLDSALAPLVGLLLDREILELIVV
                     DDGSTDSTAWIAKEAGARVVPSGGRLGPAGGRNVGAALAKGNVIWFVDADVVVHPDGA
                     RVLKDAFERTKAAAVFGAYDEFPAATNFLSQYKNLAHRHHHVRSGRERETFFAGCGAV
                     RTAAFRAIGGFDAKRYPKPSIEDIELGVRLRRRGFMIRLEPDLQARHLKSWDFADMVR
                     TDILQRTVPYVRLLGDHRVPDALRISPLEWVRVAIAWGFLLSVLTFGMGFLSWWIPLA
                     LLAAAIGLNLNLVTLFYRANGIWFALRAILFHQFHYAYTSAAYVACRLGWTPDRPREA
                     KGTGAT"
BASE COUNT          262 a          614 c          630 g          304 t
ORIGIN      
        1 ctcgccggtg atcgggttgc agtcgaggct gccgacctgc tggaaatagg tgaactgcgt
       61 gatctccatg ccgttcagcc acacctccca gccgagcccc cacgcgccga gcgtcgggtt
      121 ctcccagtcg tcctcgacga aacgcacgtc gttcttcgcg aggtcgaagc cgagcgcctc
      181 gagcgagccg aggtagaggt cgaggatgtc gggtggcgaa ggcttcagga cgacctggaa
      241 ctggtggtgc tggaacagcc ggttcgggtt ctcgccgtag cggccgtcct tgggtcggcg
      301 cgacggctgc acgtaggcgg cgcgccacgg ctcggggccg agcgcgcgca ggaacgttgc
      361 ggtatgcgac gtgcccgcgc cgacttccat gtcgtagggc tgcaaaagcg cgcagccgcg
      421 cgagccccag tactcctgga gtcgaaggat gacctgctgg aacgtgagca tgacgccggg
      481 tcgcgtagcg ctaaaccatt gattctaccg tctcgaccgt gcgtcgcccg tggtcaccgc
      541 gtcaggcgcc ctccgcggta gcatgcggcc atagtcagtg gaggggcagt ggccgaatcg
      601 ctcgaaccgg tgatcagcgt cgtcgttccg gcgtacaacg ccgagcgcac gctggactcg
      661 gcgctcgcgc cgctggtcgg cctgctgctg gaccgcgaga tcctcgagct gatcgtcgtc
      721 gacgacggat cgaccgactc gaccgcgtgg atcgcgaagg aggccggcgc gcgcgtcgtg
      781 ccgtcgggcg gccgcctcgg cccggccggc ggacgcaacg tcggcgccgc gctggcgaag
      841 ggcaacgtga tctggttcgt cgacgccgac gtcgtcgtgc atcccgacgg cgcgcgcgtg
      901 ctgaaggacg cgttcgagcg gaccaaggcg gcggcggtgt tcggcgctta cgacgagttc
      961 ccggcggcga ccaacttcct gtcgcaatac aagaacctcg cgcatcgtca ccaccacgtg
     1021 cgctcggggc gcgagcgcga gaccttcttc gccgggtgcg gcgccgtgcg caccgccgcg
     1081 ttccgcgcga tcggcggctt cgatgcgaag cggtacccga agccgtcgat cgaggacatc
     1141 gagctcggcg tgcgcctgcg ccggcgcggc ttcatgattc gcctcgagcc cgacctgcag
     1201 gcgcgtcacc tcaagtcgtg ggatttcgcg gacatggtac ggacggacat cctccagcgc
     1261 accgtcccgt acgtgcgcct gctcggggat caccgcgtgc cggatgcgct gcggatttcg
     1321 ccgctcgaat gggtgcgcgt ggcgatcgcg tgggggttcc tgctctccgt gctcactttc
     1381 ggcatgggct tcctgagctg gtggatcccg ctcgcgctgc tcgccgctgc gatcggcctc
     1441 aaccttaacc tggtcacgct gttctaccgc gccaacggca tctggttcgc gctgcgggcg
     1501 atcttgttcc accagttcca ctacgcgtac acgtcggccg cgtacgtcgc gtgccggctc
     1561 ggctggacgc cggatcggcc gcgcgaggcc aagggaacgg gcgcgaccta gggcctgtga
     1621 gcgctaccgg gacgctcgcg cccgtgccgc gctggccgca gggctaggcg cgaaggaggc
     1681 catgtgcttc cagcacataa cgactgagca acaacgccct gcggccagcg cggcatgggc
     1741 cctccgggtg atctcagcaa atggccgccc tcatcgttgc acatcctcgc cgtagttgcg
     1801 ctacggcttg
//
LOCUS       DATBSZ010000195         6115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2014137, whole genome shotgun sequence.
ACCESSION   DATBSZ010000195 DATBSZ010000000
VERSION     DATBSZ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014137"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VIG70_12385"
     CDS             <1..406
                     /locus_tag="VIG70_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY8251395.1"
                     /translation="MRKDAESNGTPQWAAADDDRITRVGRFLRKSRIDELPQIFNVLK
                     GEMSFVGPRPERTVFVDQLAKQIPFYALRHSVKPGIIGWAQVRYSYGASVDDAVEKLQ
                     HDLYYVKNNSLFLDVMIMIATAEVVLWGRGAR"
     gene            498..755
                     /locus_tag="VIG70_12390"
     CDS             498..755
                     /locus_tag="VIG70_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251396.1"
                     /translation="MKVLSKVNVLAERNGWSIDRAQGFVDGEMARRRSTAPSIYARVG
                     IDDYSMGFRAGYYERGTSQPKGAAALLGSALNDNAAVGGGD"
     gene            complement(775..2004)
                     /gene="gspF"
                     /locus_tag="VIG70_12395"
     CDS             complement(775..2004)
                     /gene="gspF"
                     /locus_tag="VIG70_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEY8251397.1"
                     /translation="MPAYSYVAVDAEGRTRRGVVEAEAPRQARANLRSSGLVPMEVSA
                     VEPEAAAPQGGGWRWGRGRQRLGGAALALVTRRFALLLEAGLTVEQCLDALIQQAQDE
                     ASTRILSAVRGEVLAGHSIADALERFPGSFPDIYRALVGTGEQSGDLAGVMSRLADYL
                     ESRRAVSQHAGLALLYPAIVASVALAIVIGLLTYVVPQVVEVFQNSRQELPFLTRALL
                     WLSGALQGNLLAIAVATAALIAALRFAWSREAWRARWQARLLRVPGIGSLLLGIGTAR
                     VASTLAILVGAGVPLLQALAASAKVVGSLPLRGAVEEVARLVREGSPLHRALDSRKVF
                     PPIFIHLVASGESSGRLAHMLAQAAKHQEMENEHRVRLLTGILEPAIIVAMGIVVLLV
                     VLAILMPIIEMNQLVRR"
     gene            complement(2010..3503)
                     /gene="gspE"
                     /locus_tag="VIG70_12400"
     CDS             complement(2010..3503)
                     /gene="gspE"
                     /locus_tag="VIG70_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEY8251398.1"
                     /translation="MAEPRESPVRLVSYQFAKAQGALLLELSTEEATVCLREGARAQA
                     LAELRRVLGVPIRVARVEPPEVFERQLAEAYTRANQSAAYVADNLEQDMDLSRLVREM
                     PRVEDLLDSENDAPIIRMINVLLTQALRQRASDIHIEPYESRSVVRFRVDGTLQNVVE
                     PHRALHAAMLSRIKVMANLDIAEKRLPQDGRISLRLAGRQVDVRVATLPTSHGERAVL
                     RLLDKEDGKLELPALGMAPGTLASVDKLIHQPHGIVLVTGPTGSGKTTTLYAALSRID
                     ALGQNIMTVEDPVEYELAGVAQTQVNARIGMTFARALRSILRHDPDVVMIGEIRDLET
                     AQIAVQASLTGHLVLATLHTNDAISATTRLTDMGIEPYLLASSLLGVLAQRLVRKLCP
                     SCKRTFAPTDADLAELGGRAGVGELFGPAGCDACHGSGYAGRTGIYELFVVDEEAKRL
                     IHAADGEQRLREHARAQGMQSLREDGMRWVVSGATSLEELVRVTRES"
     gene            complement(3509..5530)
                     /gene="gspD"
                     /locus_tag="VIG70_12405"
     CDS             complement(3509..5530)
                     /gene="gspD"
                     /locus_tag="VIG70_12405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEY8251399.1"
                     /translation="MRRASLAMLVAAMLFAGTARSQGEPVTLNFVNADIEAVAAAVGK
                     MTNRNFIIDPRVKGTINIVSARAVPSSAVYGIFLSALRLQGFTAVESGGVTKILPEAD
                     AKLHGGVVGSRGAASGDQLQTQVYTLQYTSAAQLMPVLRPIISPNNTIAAFVGNNSLV
                     VTDYGDNLRRIERIIEAIDRPGGGGEPAVIPLRFAAVADVMQTLNRVLPDSVAGTPGQ
                     AGAIDPSARLYVAQDPRTNSIVVRSENPARVEQVRALVRQLDVQTGTAANVHVVYLKN
                     AEATRVAQTLRAILSGEVAAPVPTSAPAGAPPGAPQGTGGMAQPPIGQWGGAQSPIAQ
                     GGGMVQADPASNALLISAPDAVFAHIRAIIDKLDVRRAQVYVEALIVEMTADKAAEFG
                     VQWQSLPDVSTSATRGFGGTNFQGTGTGANILNASVNPLTLGTGINVGVVRGQVTIPG
                     IGVVTNLAVLARALETDAKANILSTPNLLTLDNEEARIIIGQNIPLVTGQYAQTGAAT
                     TVTPFQTIERRDVGLTLRVKPQISEGGTVRLQIYQEASAVRDTTLAGVTLDKRAIEST
                     VLVDDGQIIALGGLVQDDVRAGIEKIPLLGDIPLLGYLFRYETRKQTKTNLMVFLRPV
                     VLRDGVAHSNASAERYRQMIGEQRETKPPPHLILPDYPAPELPPLPGDD"
     gene            complement(5530..6012)
                     /locus_tag="VIG70_12410"
     CDS             complement(5530..6012)
                     /locus_tag="VIG70_12410"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEY8251400.1"
                     /translation="MRWMKHEAWTDLLELVLVAALGVGLAHVTWLAWAPRTAAAPSAF
                     GESAQPQAGAAAARQMFGAAGSVSSAKPVVTASGLVLLGVFSRPEPRAGRAILRVQGS
                     RPALVAAGEPIADGVELQEVHADHVIVLRQGMPERIELERGGSRASRPPASSPASAGK
                     "
BASE COUNT          932 a         2133 c         2113 g          937 t
ORIGIN      
        1 catgcgaaaa gacgcggaga gcaacgggac tccccagtgg gcggcggctg acgacgaccg
       61 catcacccgg gtggggcggt tcctgcgcaa gagccgcatc gacgagctgc cgcagatctt
      121 caacgtgctc aaaggcgaga tgagcttcgt aggcccgcgg cccgagcgga cggtgtttgt
      181 cgaccagctc gccaagcaga ttccgttcta tgcgctgcgc cacagcgtca agccagggat
      241 catcggctgg gcgcaggtgc ggtactcgta cggcgcatcg gtcgacgacg ccgtggagaa
      301 gctccagcac gacctgtact acgtgaagaa caacagcctg tttctcgacg tcatgatcat
      361 gatcgccacc gcggaggtcg tgttgtgggg aagaggcgcg agataacaac gagtcggccc
      421 ggcgcagcag cgcacgcggc ccacggtaag cggtgttggg agaggagttg ccgatgatgc
      481 agtctatggg gggaaacatg aaggttttgt cgaaagtgaa cgtgctggcg gaaagaaacg
      541 gctggtcgat cgatcgcgcg cagggtttcg tcgacggcga gatggctcgc cgccgcagca
      601 cggcgccctc gatctacgcg cgggtcggga tcgatgacta cagcatgggc tttcgcgccg
      661 gctactatga gcgcgggact tcgcagccca aaggcgccgc cgcgctgctt ggcagcgcgt
      721 tgaacgacaa cgctgcggtc ggaggcggcg actagcggga ccggccccgc gcaatcacct
      781 gcgcaccagc tggttcatct cgatgatggg catcaggatg gccaggacga cgagcagcac
      841 cacgatgccc atcgccacga tgatcgcggg ctcgaggatg ccggtgagca ggcgcacgcg
      901 gtgctcgttc tccatctcct gatgcttcgc cgcctgcgcc agcatgtgcg cgaggcgccc
      961 gctcgattcg ccagaagcca ccaggtggat gaagatcggc ggaaagacct tgcggctgtc
     1021 gagcgcgcgg tgcagcgggc tcccctcgcg caccaggcgc gcgacctcct ccaccgcgcc
     1081 gcgcaagggc aggctcccta ccaccttcgc gctcgccgcg agcgcctgaa ggagcggcac
     1141 tcccgcgcca accaggatgg cgagcgtgct cgcgacgcgc gccgtgccga tgccgagcaa
     1201 tagcgacccg atccccggca cgcgcaggag ccgcgcttgc cagcgcgcgc gccatgcttc
     1261 gcgcgaccag gcgaagcgca gggcggcgat cagcgcggcg gtggctacgg cgatggcgag
     1321 gaggttcccc tgcagcgcgc cgctcagcca tagcagggca cgcgtgagga acggcaactc
     1381 ctgccggctg ttctggaaca cctccactac ctgtgggacg acgtaggtga ggagcccgat
     1441 cacgatcgcg agcgccaccg acgccacgat ggcgggatag agcagcgcga gccccgcgtg
     1501 ctgggacacg gcgcgccggc tctccaggta gtcggcgagc cgcgacatta cgcccgcgag
     1561 atcacccgac tgctccccgg tgcccacgag cgcccggtag atgtcgggaa acgagcccgg
     1621 gaagcgctcc agcgcgtcgg cgatgctatg gcccgcgagc acctcgcccc gcaccgcgct
     1681 caggatacgg gtgctcgcct catcctgtgc ctgctggatg agcgcgtcga ggcactgctc
     1741 caccgtcagc cccgcttcca gcagcagcgc gaagcgccgc gtgacgaggg caagcgccgc
     1801 gccgccgagg cgctgccggc cccttcccca gcgccagccg cctccctgcg gcgcggcggc
     1861 ctcgggctcg accgccgaca cctccatggg gacgaggccc gagctgcgca ggttcgcgcg
     1921 cgcctgccgc ggcgcttccg cctccactac accgcgccgg gtgcggcctt ccgcgtcgac
     1981 tgcgacgtag ctgtaggccg gcatgccgct caggactcgc gcgtcaccct gacgagctct
     2041 tccagcgagg tcgccccgga gaccacccag cgcatgccgt cctcgcgcag gctttgcatg
     2101 ccctgcgccc gcgcgtgctc gcgcagccgc tgctcgccgt cggccgcatg gatgaggcgc
     2161 ttggcctcct catcgacgac gaagagctcg tagatgccgg tgcgccccgc gtagcccgag
     2221 ccgtggcagg catcgcagcc cgccgggccg aagagctccc ccaccccggc gcgcccgccg
     2281 agctcggcca gatcggcgtc ggtcggcgcg aaggtgcgct tgcacgatgg gcagagcttc
     2341 ctcaccaggc gctgcgccag caccccgagc aggctcgagg cgaggaggta gggctcgatc
     2401 cccatgtcag tcaggcgcgt ggtggcgctg atcgcatcgt tcgtgtgcag ggtggcgagc
     2461 accaggtggc cggtcaggct cgcctgcacc gcgatctgcg cggtttccag gtcgcggatc
     2521 tcgccgatca tgaccacgtc ggggtcgtgc cgcaggatcg agcgcagggc tcgcgcgaac
     2581 gtcatgccga tgcgcgcatt gacctgggtc tgcgccaccc cggcgagctc gtactccacg
     2641 gggtcttcca cggtcatgat gttttgcccc agggcgtcga tgcgcgagag cgccgcgtac
     2701 agggtggtgg tcttgcccga gcccgtaggc ccggtcacca gcacaatgcc gtgcggctgg
     2761 tggatgagct tgtccacgct cgccagcgtg ccgggcgcca tgccgagggc cggcagctcg
     2821 agcttgccgt cctccttgtc gagcagccgg agcaccgcac gctcgccgtg gctcgtcggc
     2881 agcgtggcca cgcgcacgtc gacctggcga cccgcgaggc gcagcgagat gcgcccgtcc
     2941 tgcggcagcc gcttctccgc gatgtcgagg ttggccatca ccttgatgcg cgagagcatg
     3001 gccgcgtgca gcgcacggtg cggctctacg acgttctgca gcgtgccgtc cacccggaag
     3061 cggaccaccg agcgcgactc gtagggctcg atgtggatat cggaagcgcg ctgccgcagc
     3121 gcctgcgtga gcagcacgtt gatcatgcgg atgatgggcg cgtcgttctc cgagtcgagc
     3181 aggtcctcga ccctgggcat ctcgcgcacc aggcgcgaga ggtccatgtc ctgctcgagg
     3241 ttgtcggcga cgtaagcggc cgactggttg gcgcgcgtat aggcctccgc cagctgccgc
     3301 tcgaagactt ccggcggctc cacgcgcgcg acgcggatcg gcaccccgag gacgcgccgc
     3361 agctcggcga gcgcctgcgc cctcgcgccc tcgcgcaggc agacggtcgc ctcctccgtc
     3421 gagagctcga gcagcagcgc gccctgcgcc ttggcgaact ggtaggacac caggcggacc
     3481 ggcgactcgc gtggttctgc catgccgctc agtcgtcgcc cggcaggggc ggcagctccg
     3541 gagccgggta atccggcagg atgaggtgcg gcgggggctt cgtttccctc tgctcgccga
     3601 tcatctgccg gtagcgctcg gcggaggcgt tgctatgcgc cacgccgtcg cgcagcacga
     3661 ccggccgcag gaacaccatg aggttggtct tcgtctgctt gcgcgtctcg tagcggaaca
     3721 agtacccgag gagcggaatg tcgccgagca gcggtatctt ctcgatcccg gcgcgcacgt
     3781 cgtcctgcac caggccgccc agcgcgatga tctggccgtc gtcgaccagc accgtggatt
     3841 cgatggcgcg cttgtcgagg gtgacgccgg cgagggtggt gtcgcggacc gccgaggcct
     3901 cctggtagat ctgcagcctg accgtgcccc cttcggagat ctgcggcttc acgcgcagcg
     3961 tcaagccgac gtcgcgccgc tcgatggtct ggaacggcgt gacggttgtc gcggcgccgg
     4021 tctgcgcata ctgcccggtg acgagcggaa tgttctggcc aatgatgatc ctggcttcct
     4081 cgttgtccag cgtgagcagg ttgggcgtag agaggatgtt ggctttggcg tcggtctcga
     4141 gggctcgggc gagcacggcg aggttggtca cgacgcctat gccgggaatc gtgacctggc
     4201 cccggactac gccgacgttg atgccggttc ccagggtgag ggggttgacc gacgcattca
     4261 ggatgttggc gcccgttccg gtgccctgga agttggtgcc gccgaacccc cgggtcgccg
     4321 aagtgctgac atccgggagc gattgccact gcacgccgaa ctcggcggcc ttgtccgccg
     4381 tcatctcaac gatcagcgct tcgacgtaga cctgcgcgcg gcgcacgtcg agcttgtcga
     4441 tgatcgccct gatgtgcgcg aacaccgcat cgggcgccga aatgaggagc gcgttcgacg
     4501 cggggtccgc ctgcaccatg ccgcccccct gcgcgatagg ggactgcgcg ccgccccact
     4561 gcccgattgg gggctgcgcc atgccgccgg tgccctgcgg ggcgcccggc ggggcgcccg
     4621 ccggcgcgct cgtgggcacc ggtgccgcca cctctccgga gagaatggcg cgcagcgttt
     4681 gcgccacgcg cgtcgcttcg gcgttcttca ggtagacgac gtgcacgttg gcggcggtgc
     4741 cggtctgcac gtcgagctgg cgcacgaggg cgcgcacctg ctcgacgcgc gccggattct
     4801 cggagcgaac cacgatgctg ttcgtgcgtg ggtcctgggc cacgtacagg cgcgccgaag
     4861 gatcgatcgc ccccgcctga cccggggtgc cggccacgct gtcgggcagg acgcggttca
     4921 gggtctgcat cacgtcggcg acagccgcga agcgcagggg tatgactgcc ggctccccgc
     4981 cgccaccggg gcggtcgatc gcctcgatga tgcgctcgat gcggcgcagg ttgtccccgt
     5041 aatcggtaac caccagcgag ttgttgccga cgaacgccgc gatcgtattg ttcgggctga
     5101 tgatcgggcg cagcactggc atcagctgcg cagccgaggt gtactgaagc gtgtagacct
     5161 gggtctgcag ctggtccccg cttgccgcgc cgcgcgaccc caccaccccg ccgtgcagct
     5221 tcgcgtcggc ctccggcagg atcttggtca ccccgccgct ctccactgcg gtgaagccct
     5281 gcagccgcag cgccgacagg aagatgccgt acaccgcgga gctcggcacc gcccgcgccg
     5341 agacgatgtt gatcgtgccc ttcacccgcg gatcgatgat gaaattgcgg ttggtcatct
     5401 tgccgaccgc ggcggcaacc gcctcgatgt cggcgttgac gaagttgagc gtcaccggct
     5461 cgccctgcga gcgcgcggtg cccgcgaaga gcattgcggc aacgagcatc gcaagcgatg
     5521 cgcggcgcat catttgccgg ccgaagcggg cgaggacgcc ggcggccgcg atgcacgcga
     5581 cccgccgcgt tccagctcga tgcgctcggg catcccttgg cgcagcacga tcacgtgatc
     5641 cgcatgcact tcctgcagct cgacgccgtc ggcaatcggc tcgcccgccg cgacgagcgc
     5701 cggccggctg ccctgcactc tgaggatggc gcggcccgcg cgcggctccg gcctggagaa
     5761 cactccgagg agcacgaggc cgctggcagt caccacgggc ttcgcgctcg acacgctgcc
     5821 ggccgcaccg aacatctgtc gcgccgcagc tgcgcccgct tgcggctgcg cgctctcacc
     5881 gaacgcactc ggtgcagccg ccgtgcgcgg cgcccacgcg agccaggtca cgtgcgcgag
     5941 ccccacccca agcgccgcga cgagcacgag ctcaagcaaa tcggtccagg cttcgtgctt
     6001 catccagcgc atcaagttct tcttctatcc ccgcgtgtga taaacgagaa aatggtatcc
     6061 cccccaccct caaggtatag tacaccgtcc caatcaccgg gtcccagtgt gaacg
//
LOCUS       DATBSZ010000196        22759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1126058, whole genome shotgun sequence.
ACCESSION   DATBSZ010000196 DATBSZ010000000
VERSION     DATBSZ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 22759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22759
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126058"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..298
                     /locus_tag="VIG70_12415"
     CDS             32..298
                     /locus_tag="VIG70_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251401.1"
                     /translation="MNLGLRDAWDLAQIMREARDPGDAALLARFAARRRLDAGAAIRV
                     TDLLAQAFLRSNPFMGAALTALDIFPAPRRFFARRMIFGPSALP"
     gene            382..1350
                     /gene="dusB"
                     /locus_tag="VIG70_12420"
     CDS             382..1350
                     /gene="dusB"
                     /locus_tag="VIG70_12420"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY8251402.1"
                     /translation="MRIGAHALSNGLFVAPMAGITDRPFRRLARRFGAALAVSEMVSS
                     RPELRESRKTRLRLDHAGEPGPIAVQIAGSDPQMLAEAARFNVAGGAEIIDINMGCPA
                     KKVCNAAAGSALLSDEDLVAKILEAVVAAVDVPVTLKIRTGPAPGRRNALRVARIAES
                     AGVQALAIHGRTRACMFEGRAEFDTIAEVKAAVRIPVIANGDVQSPEDARRVLEATGA
                     DGVMIGRAAQGRPWFFREVAHYLATGERLAPPPAREIGAVLAEHLEGLYSLYGAEQGA
                     RVARKHIGWTVRALPGGEALRASVNTLHAAREQWAAVNDYFERLAA"
     gene            1347..1586
                     /locus_tag="VIG70_12425"
     CDS             1347..1586
                     /locus_tag="VIG70_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8251403.1"
                     /translation="MSKGNELSDAVKRSLERYFKDMDGEKPTSIYEMVLKNIERPMIE
                     TVLGHAKGNLTHAAAMLGINRATLRTKMHQLRIKG"
     gene            1586..3145
                     /gene="purH"
                     /locus_tag="VIG70_12430"
     CDS             1586..3145
                     /gene="purH"
                     /locus_tag="VIG70_12430"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY8251404.1"
                     /translation="MTKVKRALVSVSDKSGVVEFARGLAALGVELLSTGGTAKLLLKE
                     GLPVVEVSAYTGFAEMLDGRVKTLHPKIHGGLLGRRDDPTHIKEMRAAGIEPIDLLVV
                     NLYPFQAAVADPECRFDDAIENIDIGGPAMLRSAAKNHASVAAVVDPADYSRVLQEIR
                     QQGGVSEATRFGLATKVFAHTAAYDGAIANYLTSLDEHRRRLDYAEVLTLQFCKVDDL
                     RYGENPHQSAAFYRDLHPVAGTLAQYRQLQGKELSYNNIADADAAWECAKSLAEPACV
                     IVKHANPCGVAIGTTLVGAYESALRCDPVSAFGGILAFNRALDLATAQAIGKQFAEVV
                     VAPRIEADAQKELSAKENLRVLEVPLSHEAQAHDYKRVGGGLLVQSSDSQLLVRDQLK
                     TVTRKKPTEDQVRDLLFAWRVAKFVKSNAIVFCRGGATVGIGAGQMSRLDSVRVAVMK
                     AQEAKLSLDGAVVASDAFFPFRDGLDALADAGATAVIQPGGSLRDAEVIAAADERGLA
                     MLFTGMRHFRH"
     gene            3147..4421
                     /gene="purD"
                     /locus_tag="VIG70_12435"
     CDS             3147..4421
                     /gene="purD"
                     /locus_tag="VIG70_12435"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY8251405.1"
                     /translation="MKLLVIGSGGREHALAWKLAHSPRVQKVFVAPGNGGTATENGVE
                     NVAITQIPDLVSFSKKEKIHLAVVGPEAPLAAGIVDTFQEAGLKIFGPRRAAAQLEAS
                     KDFAKRFMARHGIPTALHRTFDSAGEAKDYVAAQGAPIVVKADGLAAGKGVVVAASVP
                     EAHAAIDQMMTQKSLGAAGSRLVVEEFLEGEEASFIVMSDGSHVLALATSQDHKRLRD
                     GDKGPNTGGMGAYSPAPVVTPKVHARVMREVIVPAIQGMAQDGAPYVGFLYAGLMIDE
                     SGAPRTLEFNCRLGDPETQPIILRLKSDLLDLIEHALEGTLDEAEAQWDRRAALGVVV
                     AAAGYPEEPRKGDVISGLPKPDADCRVFHAGTRLDGKTLVTSGGRVLCVTALGDSVKR
                     ARARAYEALDGIRFEGMQYRKDIAHRALKKSS"
     gene            4423..5298
                     /gene="hemF"
                     /locus_tag="VIG70_12440"
     CDS             4423..5298
                     /gene="hemF"
                     /locus_tag="VIG70_12440"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEY8251406.1"
                     /translation="MDRQAVHEYFAGLQERIVSALEVVDRKGFVRDSWQRPEGGGGTT
                     CMVENGQVFERGGVNLSRVQGRQLPPSASAARPELAGRAYDAMGVSLVLHPRNPYCPT
                     VHMNVRFFTAGDIWWFGGGMDLTPYYGFEEDARHFHSVCRDALAPFGADYYPRFKKWC
                     DEYFHLRHRGEPRGIGGIFFDDLAGSSSFALVQSVGDHFLTAYLPILERRKHLPFGEL
                     ERRFQAYRRGRYVEFNLVYDRGTLFGLQSGGRTESILMSLPPRAEWRYDWKPDPGSPE
                     EKLYTDFLKPRDWLS"
     gene            complement(5291..6481)
                     /locus_tag="VIG70_12445"
     CDS             complement(5291..6481)
                     /locus_tag="VIG70_12445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEY8251407.1"
                     /translation="MRALFWLLVVSAAAVALVLLARVDSGYVLFFYPPWRVEMSVVFF
                     AVAAAASFLVLYLLFRLLGHALALPATVRVWRARRRGARAHAALAAALQAYYEGRYAR
                     AEKEAGIAFDSGPTPGVAALLAARAAHQMRDFERRDRWLERADGSGDALQAARLVSRA
                     ELALEDRDFATARDALRNLHGAGPKHVATTRLLLRAERGAGAWDEVLRLASQLSKRDA
                     ISPALAEEYKVQATVELLARSADDAGAFESRWRGIAAGDRTQPRIAAAGARHATALGK
                     AAMAREIIENALAAEWVPTLVSLYGNLTLGIKDERVSEARARIERAERWLLERSRDAQ
                     LLATLGRLCAQAELWGKARSFLEASLSFEESRAARVELAHLAERLGQADEAQRHFRRA
                     AELS"
     gene            complement(6478..7488)
                     /locus_tag="VIG70_12450"
     CDS             complement(6478..7488)
                     /locus_tag="VIG70_12450"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY8251408.1"
                     /translation="MAEDSAKPRTLATLLLLIAIALAAGLATLYWLDARSRIGATQEE
                     LARRLRDIENDAREARSIARQSQEGMREAQVKVLQLESRLAESQSQQLALEALYQEMS
                     RNRDEWQLAEIEQVLAIAQQQLQLAGNVRAALLALQLAEARVAKADRPQFLPVRRALA
                     RDIERLKALPVLDIAAMSSRIDTLVAGVDSLPLAFEEKSVAVKNETKDSERGFWSRLG
                     SEVWSEVKQLVVVRQVGSNEPPLLPPPQAYFLRENLRLRLLNARLSLLARDEAGYRED
                     LRAAQAWIKRYFDPRSKQTVDAQTQLKQLSSTSISFEMPSISESLDAVRGFKSRRTRS
                     AG"
     gene            complement(7481..8215)
                     /locus_tag="VIG70_12455"
     CDS             complement(7481..8215)
                     /locus_tag="VIG70_12455"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY8251409.1"
                     /translation="MDSNSLLDRGILVTRPREQAARLARLIEEAGGRAHVFPAIAIED
                     LPAPAALARVEQFDLAVFVSPTAVGKTLSRMGAWPAAVRAAAVGAGTRRELERYGVTN
                     VIAPHEGADSEALLGESELGKVAGKRIVIFRGEGGRALLGDTLRERGAQVEYAACYRR
                     RLPEKGPVDWRPEEIDAITVSSAEGLANLFEMLDPAWLRARPLFVPHPRIAGDARARA
                     VPEVLVAGPSDEEMRDALVAYFRSHG"
     gene            complement(8205..9116)
                     /gene="hemC"
                     /locus_tag="VIG70_12460"
     CDS             complement(8205..9116)
                     /gene="hemC"
                     /locus_tag="VIG70_12460"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY8251410.1"
                     /translation="MKKLVIATRRSRLALWQAEHIATLLKRFHAGLKVELLPLSTRGD
                     ELIDRRLDQAGGKGLFVKELETAMAEGRADLAVHSIKDVPADLPAGFALAAITAREDP
                     RDVLVSKKFKSLREMPEGATVGTSSLRRSSQISERFPRLEIRLLRGNVDTRISKLDRG
                     EYDAIVLAAAGLTRLGLDVRITARLEVDEMLPAPGQGALGIECRADGAEVAALLAPLA
                     DRATTICVRAERAVSRALGGSCTLPLAAHAEYLEKRLRLRALVASADGKRVIRCDLQG
                     DPADPQGLGECAADDLRRQGAAEILGL"
     gene            complement(9113..9853)
                     /locus_tag="VIG70_12465"
     CDS             complement(9113..9853)
                     /locus_tag="VIG70_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY8251411.1"
                     /translation="MTLKVFIVDDEAPARERLRALLGDIAAEVATSVVGEARHGVEAV
                     ERVPASGAEVVLLDIKMPGMGGLEVARHLGALERAPRIVFVTAHERHAVEAFELNALD
                     YLLKPVRAERLAAALQKASVPPQERLAKAAEAPREYLSVTERSRIVLVPVRDILFLRA
                     EQKYVTLRTRDREHLVEESLVALEREFAVRFVRIHRNCLVARAAIRGFERAPGEDEEA
                     HWLVVLEGLDERLPVSRRQWPLLRDLVT"
     gene            complement(9850..10752)
                     /locus_tag="VIG70_12470"
     CDS             complement(9850..10752)
                     /locus_tag="VIG70_12470"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8251412.1"
                     /translation="MFAGLVFNTATFAQALDRFVQAAALLEPLLLVIIAVLFALSGTL
                     RRLPYWAGCVAVVALVAAVVAGYLATIGATEALARVLILTVLITAALLAYLRLLSKAH
                     SPALAEARLQALQARIRPHFLFNSLNAVLALIRRDPQRAERALEDLADLFRTLMSDPR
                     AFVRLADEISLLERYAGLEQLRLGERLRVTWELDGAPPDALLPPLVLQPLLENAVYHG
                     VEPGTGAGEVLVRIERRGNRVLALIENPFLEAEAGRAGNRMALENIRERLQLFFDAEA
                     RITTVAADGRYRVEIEIPYKIRMA"
     gene            10879..12264
                     /gene="argH"
                     /locus_tag="VIG70_12475"
     CDS             10879..12264
                     /gene="argH"
                     /locus_tag="VIG70_12475"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY8251413.1"
                     /translation="MTRKPSPWSARFSEPVDERVKRFTASVSFDQRLAKYDIQASRAH
                     ARMLAAQRILTRRDLAAIQRGLARIEREIAAGRFRWSLEAEDVHLNVERRLTKLVGDA
                     GKRLHTARSRNDQVATDLRLWLRAEIDELRALIAALEHALLDQAARHAALVMPGYTHL
                     QVAQPVTFGHHLLAYVEMLERDRQRLLEARARVNCLPLGAAALAGTTFPIDRRRVARE
                     LGFEALCENSMDAVSDRDFAVEFCACAALAMVHLSRLAEELVLWASPRFGFVRLPEAY
                     STGSSIMPQKRNPDVPELVRGKTGRVVGALVALLTLMKAQPLAYNKDNQEDKEALFGA
                     VDTLKDCVSIFIGLVEGLEPLPRAMRAAVLEGHATATDLADYLVRKGVPFRDAHGVVA
                     RAVREAERAGVDLASLPIKKLKEFSARIDADVAKVLTPEGSVAARKHPGGTAPVAVRR
                     AVARARKRLRK"
     gene            12261..13556
                     /locus_tag="VIG70_12480"
     CDS             12261..13556
                     /locus_tag="VIG70_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8251414.1"
                     /translation="MSRREMLAQIGKYPVLRKLGEGATSEVYLCSDPFNLRDVAIKVA
                     FPESFQDPARGKVYRKLFLTEAKLAGKLVHPHICQIYDAVAEEKLHYLVMEYVDGGTL
                     EKYCNGQALLPVERTVEIIFKCTRALEFAHKLGITHRDIKPANILLTGETGVKITDFG
                     TALIATAETTQVAGVGSPAYMSPEQVKDLPLDHRTDIYSVGVVMYHLLTGRLPFQAAN
                     NYTLIYQITNAEPDASTSLRRDIPPAVDAIVRRAMAKDLNKRYAHWGDFSDDLGAAFR
                     SEHFGARKVQSFADSDRFETLRRLQFFERFSDAELWEVASISTWRHAPAGEILMKEGE
                     PGDYFCILAQGEVKITRHGKLLNVLRAGEPFGEMAYLSRNEHARGADVTVSADASIIS
                     VPTNRLNQASEGCRHKFDRAFMEILVERLTMANLRVSGV"
     gene            13612..14862
                     /locus_tag="VIG70_12485"
     CDS             13612..14862
                     /locus_tag="VIG70_12485"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8251415.1"
                     /translation="MRKLGEGATSEVFLCQDPFRGREVAVKRIFPESLRDPARGRLFR
                     KLFFTEASLAGKLDHPHIAQIYDAGIGEDSGYIVMEYVPGGTIERFCAPDNLLPVEQT
                     VEIIFKCARALAYAHQQGVTHRDIKPGNVLYAAAPTDVRISDFGLALNVGAETTQITG
                     VGSPAYMSPEQIREELVDHRTDVYSLGVVMYQMLCGKLPFQGSNKFSMIYQITQSDPP
                     PPSAHRPKVPEVLDRITRRAMHKTTRARYASADELAADLVEALRTGLGRTRRNRFGDA
                     DKFAALRAMQFFKRFTEPELWEVVRVGEWERVPAGTEIVREGDPGDFFCVLVEGEARV
                     TKNRKLLTVLGQGECFGEMAYLSADRRERGATVTASKDAHIVRVRTAELEQASVNCRS
                     HFDRAFIGILVERLNLANTRLTSG"
     gene            14869..15546
                     /locus_tag="VIG70_12490"
     CDS             14869..15546
                     /locus_tag="VIG70_12490"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEY8251416.1"
                     /translation="MRFGALFILFVACGVLRAAEHEERDRALLSVAQGCARQIPGALV
                     TGVRNGVVQIKLMNQGDGAGFDRCYQEAAPRTLQALALGRLAEHAAHTSVALETAGSL
                     MFVPALVNGVQVRLLLDTGAGKTIVRPLIAQLAGIEPGRQAPLAQITVAGGGQLSVPL
                     AKARSLAINEAAVLAIEIGIYEALPDLPDVDGILGSDYLSHFTVTIDRQKGTLLLVPA
                     RRQVRSD"
     gene            15554..16354
                     /locus_tag="VIG70_12495"
     CDS             15554..16354
                     /locus_tag="VIG70_12495"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEY8251417.1"
                     /translation="MHAALRKQLGIAAAEPVSGGCIHRCYRVAIAGTQRFLKVNSGAH
                     EDAFASEADGLVALRNAGLHAPEPLAHGVAGDEAYLLLEYLELKTKGDFPALGRMLAN
                     AHRKPGPRFGWHRNNYIGSTPQQNGWCDDWAEFWITRRMKPQLALARRNGFDLEVKID
                     LGNHRPQPSLLHGDLWSGNAGFAAQGPVVFDPAVYYGDREADLAMTELFGGFPREFYK
                     AYDEAFPLPAGYEKRKHLYNLYHLLNHLNLFGGGYLSQVKSTLGLLGL"
     gene            complement(16334..16651)
                     /gene="grxD"
                     /locus_tag="VIG70_12500"
     CDS             complement(16334..16651)
                     /gene="grxD"
                     /locus_tag="VIG70_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY8251418.1"
                     /translation="MDTQQRIKEQVTGNRVVLFMKGTPQGPQCGFSALAVQVLSACGV
                     KDFHSVNVLADAEIREGIKQYANWPTIPQLYINGEFVGGSDIMREMYQSGELQKLLET
                     EKA"
     gene            complement(16662..17450)
                     /gene="prmC"
                     /locus_tag="VIG70_12505"
     CDS             complement(16662..17450)
                     /gene="prmC"
                     /locus_tag="VIG70_12505"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY8251419.1"
                     /translation="MKIVEALSASGIDGREARLLLAAATGFSEASVLAHPERQLPSDV
                     EASFSQMTARRAAGEPVAYILGRKEFYGLDLVVTPAVLIPRPETELLVDLALARKPAS
                     VVDIGTGSGAIALALKRHLSAARVVATDASASALEVAKRNAVRLALDVELRHGRWFEP
                     LAGERFEAIICNPPYVVADDPHLVDLHYEPRFAIVAGADGLDGFRMLARGAPDHLLPG
                     GWLLLEHGAGQHEAVRALLEAAGLETTASWPDLAGIPRVSGGKR"
     gene            complement(17473..18558)
                     /gene="prfA"
                     /locus_tag="VIG70_12510"
     CDS             complement(17473..18558)
                     /gene="prfA"
                     /locus_tag="VIG70_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY8251420.1"
                     /translation="MNPRLRTKLDSLVGRLGDLERLLAAENATRDMEQFKRLSREHSE
                     VSSVVSLFQSYLQAEREADEARDLASDATMKAYADEELKKARAAMERLAAELQKALLP
                     KDPNDERNLFIEVRAGTGGDESALFAGDLFRMYTRFAERKGWQVEIISESASELGGYK
                     EVIARIVGQGAFSRLKFESGGHRVQRVPKTEAQGRIHTSACTVAVLPEADAIEDVVLN
                     PAELRVDTFRASGAGGQHVNKTDSAIRVTHLPTGIVVECQDSRSQHKNREKALSVLKA
                     KIRDKQEREQQAKTASTRKSLIGSGDRSERIRTYNFPQGRVTDHRINLTLYKIERIMD
                     GELDEIIDALAHEHQAEQLASLTEEAA"
     gene            complement(18603..19781)
                     /gene="hemA"
                     /locus_tag="VIG70_12515"
                     /pseudo
     CDS             complement(18603..19781)
                     /gene="hemA"
                     /locus_tag="VIG70_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
     gene            complement(19880..20299)
                     /locus_tag="VIG70_12520"
     CDS             complement(19880..20299)
                     /locus_tag="VIG70_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8251421.1"
                     /translation="MSGFEQVEILLVEDDPEDAETTLGALRKRSLADHVHWVKDGAEA
                     LDYLFCSGAYAGRSPRHPPKLVLLDIKMPKVDGIEVLRRVKGSELRAIPVVVMTSSSE
                     ERDVLESYRLGVDRYLVKPVRVEAFVDTVSSIGLSWK"
     gene            complement(20367..20993)
                     /locus_tag="VIG70_12525"
     CDS             complement(20367..20993)
                     /locus_tag="VIG70_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8251422.1"
                     /translation="MIRIVIVDDHAPVREGVTRLLEEAPDLRVIGSFDDDTAAVRFAA
                     RAEPDVAIVGVAMRRTSGIDLARRLRAVSPKTRLLMLSMHARPEYVQQAFRAGANGYV
                     LKEAAGAEVVAAVRAVHAGKRYLSAGIALRGSGEAVVEDPLERLSAREMEVLKLVVEG
                     NTSHEVAQLLGLSPKSIDTYRSRLMTKLDVDHLTALVKFAIRRGLTSA"
     gene            21324..21476
                     /locus_tag="VIG70_12530"
     CDS             21324..21476
                     /locus_tag="VIG70_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251423.1"
                     /translation="MSSKKQNQSVVRKPAKKLTAPRVVRAQDSATRDSGTIRFGSGSA
                     PASLRK"
     gene            21489..22262
                     /locus_tag="VIG70_12535"
     CDS             21489..22262
                     /locus_tag="VIG70_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251424.1"
                     /translation="MNEARQPNPAVTFYGAIPGCRAPMRADPSVLGTLPARGFQYCEA
                     LRAASSFGWYAFSPIDFSLLWDGSEVIWTYRGAKAWYPLKSAQFPGFSRYFDDTAPKR
                     LRGFAPPFLTSTWDPSLIQVWTGLFVESAPDWSILVRPPANLQRHEGYHLYEGIVESD
                     RWFGPLFTNLRLVKTDVPIHFSTELPLVQVQPLHRSAYAEEISNHFGLVPDLADLPAH
                     AWSRYEEAIVKPSIEPRPAGAYAASVRRRRKSGCPVHAE"
     gene            22259..>22759
                     /locus_tag="VIG70_12540"
     CDS             22259..>22759
                     /locus_tag="VIG70_12540"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8251425.1"
                     /translation="MNGYIAAAIAVLIWASYPVATRAGVTGSFTPQELVTLRFGGGAL
                     LFLPYLLLQWRQVSRSAWLQGVPLTLFQGAGMGALVICGLQFAPAAHQAALGPGVSPA
                     WVALLGFVIFARRPSTRAIVGATLCATGVLALASWSGMAHDARVLAGDAMFLAASVLG
                     ALYVLQL"
BASE COUNT         3655 a         7844 c         7713 g         3547 t
ORIGIN      
        1 ccagacgttg cacccagtcg cgggccaggg cttgaacctc ggcctgcgcg acgcctggga
       61 cttggcacag atcatgcgcg aggcgcggga tccgggcgac gcggccttgc ttgcgcgctt
      121 cgccgcgcgc cgccgcctcg atgccggcgc cgcgatccgc gtgaccgatc tgcttgcgca
      181 agcttttctg cggtccaacc ccttcatggg cgcggctttg acggcgctgg atatttttcc
      241 cgcaccgcgg cgcttcttcg cgcggcgcat gatcttcggt ccgtcggcgt tgccgtaaaa
      301 atacaacaat cgggcccgag cggttttttt tgccagggaa agcggataaa atccgcttcc
      361 cttcgtatcg caatctcggc aatgaggatc ggagcccacg cgctctccaa cggattgttc
      421 gtcgctccga tggccggcat caccgaccgg ccgttccggc gtctcgcgcg gcgcttcggc
      481 gccgcgctcg cggtctccga gatggtttcg tcccgccccg agctgcgcga gtcgcgcaag
      541 acgcggctgc gcctggacca tgccggcgag ccgggtccga tcgcagtgca gatcgcaggc
      601 agcgacccgc agatgctcgc tgaggccgcg cgcttcaacg tcgccggcgg cgccgagatc
      661 atcgacatca acatgggctg cccggcaaag aaggtgtgca atgccgcagc gggctcggcg
      721 ctcctgtcag atgaagactt agtggcaaag attctggagg cggtcgtggc ggcggtcgac
      781 gtacccgtga ccctgaagat ccgcaccggt ccggctcccg ggcggcgcaa cgcgctgcgc
      841 gtcgcgcgca tcgcggagtc cgccggcgtg caggccctcg cgatccacgg ccgcacgcgc
      901 gcgtgcatgt tcgagggccg ggcggagttc gacaccatcg cggaagtgaa ggccgccgtg
      961 cgcatcccgg tgatcgccaa cggcgacgtc cagtcgcccg aggacgcccg gcgcgtgctg
     1021 gaggcgaccg gcgccgacgg cgtgatgatc ggccgcgcgg cgcaggggag gccgtggttc
     1081 ttccgcgagg tggcgcacta cctggcgacc ggcgagcggc tcgcgccgcc gccggcgcgc
     1141 gagatcggcg cggttctcgc cgagcacctg gaagggctct acagcctcta cggcgccgag
     1201 cagggcgcgc gcgtcgcgcg caagcacatc ggctggacgg tgcgcgcgct gccgggagga
     1261 gaggcgctgc gcgcgagcgt caacaccttg catgccgccc gcgagcaatg ggccgcggtc
     1321 aacgattact tcgagaggct cgccgcatga gcaaaggaaa cgagctttcg gacgccgtca
     1381 agcgctcgct cgagcgctat ttcaaggaca tggacggaga gaagcccacg tccatctacg
     1441 agatggtgct gaagaacatc gagcgaccga tgatcgagac cgtgctcggc cacgccaaag
     1501 gcaacctgac ccacgcggcg gcgatgctcg gcatcaatcg cgccaccctg cgcacgaaaa
     1561 tgcaccagct gcgcatcaaa gggtagtgac gaaggtcaag cgggcgctcg tcagcgtctc
     1621 ggacaagtcg ggcgtcgtcg agttcgcccg cgggctcgcc gcactcggcg tcgagcttct
     1681 ctccaccggc ggcaccgcca agctcctgct gaaggaaggg ctgccggtcg tcgaagtgtc
     1741 ggcctacacc ggtttcgccg agatgctcga cggccgcgtg aagacgctgc acccgaagat
     1801 ccacggcggc cttctcgggc ggcgggacga tccgactcat atcaaggaaa tgcgggcagc
     1861 ggggatcgag ccgattgacc tgctggtcgt caacctgtac ccgttccagg ccgcggtcgc
     1921 cgatcccgaa tgccggttcg acgatgcgat cgagaatatc gacatcggcg ggcccgcgat
     1981 gctgcgctcg gcggcgaaga accatgcgtc ggtggccgcg gtggtggatc cggcggacta
     2041 ttcacgggtt cttcaggaaa taagacagca aggaggggtg agcgaggcga cgcgcttcgg
     2101 gctcgcgacg aaggtgttcg cgcacaccgc cgcatacgat ggcgcgatcg ccaactacct
     2161 cacttcgctc gacgagcatc ggcggcggct cgactacgcc gaggtcctga ccctgcagtt
     2221 ctgcaaggtc gacgacctgc gctacggcga gaacccgcac cagagcgccg ctttctaccg
     2281 cgacctgcat ccggtggcgg gaaccctggc gcagtaccgc caactccagg gcaaggagct
     2341 ctcctacaac aacatcgccg atgccgatgc ggcctgggaa tgcgccaaga gcctcgccga
     2401 gccggcgtgc gtgatcgtca agcatgccaa tccatgcggg gtcgcaatcg ggaccacgct
     2461 cgtgggggcc tatgagagcg ccttgcgctg cgacccggtg tcggccttcg gcggcatcct
     2521 cgccttcaac cgcgcgctcg accttgcgac cgcgcaggcg atcggcaagc agttcgccga
     2581 agtggtcgtc gcgccgcgca tcgaagccga tgcccagaag gagctgtccg ccaaggaaaa
     2641 ccttcgcgtg ctcgaggttc cgctgtcgca cgaggcgcag gcgcacgact acaagcgcgt
     2701 gggcggaggc ttgctcgtgc aaagcagcga ctcgcagcta cttgtccgcg accagctgaa
     2761 aaccgtgacg cgaaagaagc ccacggagga tcaggtgcgc gacctgctgt tcgcctggcg
     2821 ggtagcgaaa ttcgtgaagt cgaatgcgat cgtgttctgc cgcggcgggg cgacggtggg
     2881 catcggcgcc gggcagatga gccggctcga cagcgtgcgc gtggcggtaa tgaaagcgca
     2941 ggaagcgaag ctgagcctcg acggcgccgt ggtggcctcg gacgcgtttt ttccgttccg
     3001 cgacggcctg gacgcgctgg cggacgccgg cgcgacggcg gtgatccagc ccggcggcag
     3061 cctgcgagac gccgaggtga tcgccgcggc cgacgagcgc ggcctcgcca tgctcttcac
     3121 gggcatgcgc cacttcaggc actgaagtga agctcctcgt catcggctcg ggaggccgcg
     3181 agcacgcgct ggcgtggaag ctcgcgcaca gcccgcgcgt gcagaaggtg ttcgtcgcgc
     3241 cgggcaacgg cggcaccgcg accgagaacg gcgtcgagaa cgtggcgatc acgcagatcc
     3301 cggatctggt ttccttctcg aagaaggaaa agatccacct ggcggtggtc ggtccggaag
     3361 cgccgctcgc ggccgggatc gtcgacacgt tccaggaagc cggcctgaag atcttcgggc
     3421 cgaggcgcgc cgcggcgcag ctcgaagcgt cgaaggattt cgccaagcgc ttcatggcgc
     3481 gccacggaat tcccacggcg ctgcaccgca cgtttgacag cgccggcgag gccaaggact
     3541 acgtcgccgc gcagggcgcc cccatcgtag tgaaggccga cggcctggcc gccggcaagg
     3601 gcgtggtggt ggcggcaagc gtgcccgagg cgcacgccgc gatcgaccag atgatgacgc
     3661 aaaagagcct cggcgcggca ggcagccgtc tggtggtcga ggagtttctc gagggcgagg
     3721 aggcgagctt catcgtgatg agcgacggca gccacgtcct cgcgcttgcg accagccagg
     3781 accataagcg cctgcgcgac ggcgacaagg ggccgaacac cggcggcatg ggcgcctact
     3841 cgcccgcgcc ggtcgtcacg ccgaaggtcc acgcccgtgt gatgcgcgag gtcatcgtgc
     3901 cggcgatcca gggcatggcg caggacggcg cgccctacgt cgggttcctg tacgccggcc
     3961 tgatgatcga cgagagcggc gcccccagga ccctggaatt caactgccgg ctcggcgacc
     4021 ccgagaccca gccgatcatc ctgcggctca agtcagacct gctggatctg atcgagcacg
     4081 cgctcgaggg cacgctcgac gaggcggagg cgcaatggga ccggcgtgcc gcgctcggcg
     4141 tggtggtcgc ggccgcgggc taccccgagg agccgcgcaa gggcgacgtg atcagcggcc
     4201 tgccgaagcc cgatgcggat tgccgcgtgt tccatgcggg cacccggctc gacggcaaga
     4261 ccctcgtcac cagcggcggc cgcgtgctgt gcgtgaccgc gctcggcgac tcggtgaagc
     4321 gggcgcgcgc gcgggcctac gaagcgctcg atggcattcg cttcgaaggc atgcagtatc
     4381 gcaaggacat cgcccaccgc gcgctcaaga agagctcctg aggtggaccg gcaggccgta
     4441 cacgagtatt tcgcgggctt gcaggagcgg atcgtgtccg ccctggaagt ggtcgaccgc
     4501 aagggcttcg tgcgcgattc gtggcagcgc cccgaaggcg gcggcggcac cacctgcatg
     4561 gtcgagaacg ggcaggtttt cgagcgcggc ggcgtgaacc tgtcgcgcgt gcaggggcgg
     4621 cagcttccgc cttcggccag cgcggcccgg cccgagctcg ccggccgcgc ctacgacgca
     4681 atgggcgtgt cgctcgtgct tcacccgcgc aacccttact gccctaccgt gcacatgaac
     4741 gtgcgcttct tcaccgccgg agacatctgg tggttcggcg gcgggatgga tctcacgccc
     4801 tactacggct tcgaggagga cgcgcggcac ttccactcgg tatgcaggga tgcactcgcg
     4861 ccgttcggcg ccgactacta tccgcggttc aagaaatggt gcgacgaata ctttcacctg
     4921 cgccatcgcg gcgagccacg cggcatcggc gggattttct tcgacgatct cgcgggaagc
     4981 tcttcgttcg cgctggtgca atcggtcggc gatcatttcc tcaccgccta cctgccgatc
     5041 ctcgagcgcc gcaagcacct gcccttcggc gagctggaac ggcgcttcca agcctatcgc
     5101 cgcggccgct acgtggagtt caacctggtc tacgaccgcg gcacactgtt cgggctgcag
     5161 tcgggcggcc gcaccgaatc gatcctgatg tcgctgccgc cgcgcgccga atggcgctac
     5221 gactggaagc ccgaccccgg ctcgcccgag gaaaagctct acaccgactt cctgaaaccc
     5281 cgagactggc ttagctgagc tccgccgcgc ggcggaagtg gcgctgcgcc tcgtcggcct
     5341 ggccgaggcg ctcggcgagg tgcgcgagct cgacgcgggc ggcgcggctc tcctcgaacg
     5401 acaggctcgc ttcgaggaag ctgcgcgcct tgccccacag ctcggcctgg gcgcacaggc
     5461 gcccgagcgt ggcgaggagc tgcgcgtcgc ggctgcgctc gagcaaccag cgctcggcgc
     5521 gctcgatgcg agcgcgagct tccgagactc tttcgtcctt gattcccagg gtcaggttgc
     5581 cgtagaggga cacgagcgtc ggcacccatt ccgcggcgag cgcgttctcg atgatctcgc
     5641 gcgccatagc cgcctttccg agggcggtgg cgtggcgcgc gccggcggcg gcgatgcgcg
     5701 gctgagtgcg atcgccggcc gcgatgccgc gccagcggct ctcgaaggcg ccggcgtcgt
     5761 cggccgagcg cgcgagcagc tccaccgtcg cctgcacctt gtactcctcc gcgagcgcgg
     5821 gcgagatcgc gtcgcgcttc gagagctgcg aggcgagccg cagcacttcg tcccaggcgc
     5881 ctgcgccgcg ctccgcgcgc agcaagaggc gcgtggtggc gacgtgcttc gggccggcgc
     5941 cgtgcaggtt gcgcagcgcg tcgcgcgccg tggcgaaatc acggtcctcg agcgcaagct
     6001 cggcgcggct caccagccgc gccgcctgca gcgcatcgcc cgagccgtcg gcgcgctcga
     6061 gccagcggtc gcggcgctcg aagtcgcgca tctggtgcgc ggcacgcgct gcaagcagcg
     6121 cggcgacccc cggcgtcggc ccgctgtcga acgcgatccc ggcctccttc tccgcgcggg
     6181 cataacggcc ctcgtagtag gcctggagcg cagcggcgag cgcggcatgg gcgcgcgcac
     6241 cgcggcgccg cgcgcgccag acgcgcaccg tggcgggaag cgcgagcgca tggccgagca
     6301 ggcggaacag gaggtacagg accaggaacg acgcggccgc tgccacggcg aagaacacca
     6361 ccgacatctc gacgcgccac ggcggataga aaaacagcac gtagccggag tcgacgcgtg
     6421 cgagcaatac cagcgcgacc gcggcggcgg acaccacgag cagccagaac agcgctctca
     6481 tcccgcgctt cgcgtgcgcc gcgacttgaa accgcgcacc gcatcgaggc tttcggagat
     6541 cgacggcatc tcgaagctga tcgaggtcga cgacagttgc ttgagctgcg tttgcgcgtc
     6601 gacggtctgc ttgctgcgcg gatcgaaata gcgtttgatc caggcctgcg cggcgcgcag
     6661 gtcctcgcgg tagccggcct cgtcgcgcgc caggagcgaa aggcgtgcgt tgaggagccg
     6721 caggcgcagg ttttcgcgca gaaaatacgc ctgcggcggc ggcagcagcg gcggctcgtt
     6781 ggagccgacc tggcgcacca ccacgagctg cttcacctcg ctccacacct ccgaccccag
     6841 cctcgaccag aagccccgtt ccgaatcctt ggtctcattt tttacggcga ccgatttctc
     6901 ttcgaaggcg aggggcagag agtcgacccc cgccaccaga gtatcgatgc gcgagctcat
     6961 cgccgcgatg tcgagcaccg gcagcgcctt gaggcgctcg atgtcgcgcg cgagcgcgcg
     7021 ccgcaccggc aggaactgcg gccggtcggc cttcgccacg cgcgcctcgg cgagctgcag
     7081 cgcgagcagc gccgcgcgca cgttgccggc gagctgcagc tgctgctgcg cgatggcgag
     7141 gacctgctcg atctcggcga gctgccactc gtcgcggttg cgcgacatct cctggtagag
     7201 cgcttcgagc gcaagctgct ggctctgcga ttcggcgagg cggctttcga gctgcaggac
     7261 cttgacctgc gcctcgcgca tgccctcctg cgactggcgc gcgatcgagc gcgcctcgcg
     7321 cgcgtcgttc tcgatgtcgc gcaggcgccg ggccagctct tcctgcgtcg cgccgattcg
     7381 gctgcgcgcg tccagccagt agagcgtggc gagccccgcg gcgagcgcga tggcgatcag
     7441 caggaggagc gtcgcgaggg ttcggggttt cgccgaatct tcagccatgg gagcgaaagt
     7501 atgccaccag cgcgtcccgc atttcctcgt ccgaaggccc cgcgacgagc acttcgggca
     7561 ccgcccgagc gcgcgcgtcc ccggcgatcc gcgggtgcgg cacgaagagc ggccgcgcgc
     7621 ggagccaggc gggatcgagc atctcgaaca ggttcgccag cccctccgcg gaggagacgg
     7681 taatggcgtc gatctcctcc ggcctccagt ccaccggacc tttttcggga agcctgcggc
     7741 ggtagcacgc cgcatactcg acctgcgcgc cgcgctcgcg cagcgtatcg ccgagaagcg
     7801 cgcgcccgcc ctcgccgcgg aagatcacga tgcgcttccc cgcgaccttt ccaagctccg
     7861 attctccgag caacgcctcg ctgtccgcgc cttcgtgcgg cgcgatcacg ttggtcacgc
     7921 cgtagcgctc gagctcgcgc cgcgtccccg cgccgaccgc cgcggcgcgc accgccgcgg
     7981 gccatgcgcc catccggctc agagtcttgc cgaccgccgt cgggctgacg aagaccgcaa
     8041 gatcgaattg ctccacgcgc gcgagcgccg cgggcgcagg caagtcctcg atcgcgatcg
     8101 cgggaaagac atgcgcgcgg ccgcccgcct cctcgatcag gcgcgcaagg cgcgcggctt
     8161 gctcgcgcgg acgcgtgacg agaatgccgc gatcaagcag tgaattagag tccaagaatt
     8221 tccgctgcgc cctggcggcg cagatcgtcc gcggcgcact cgccgaggcc ctgcggatcg
     8281 gccgggtcgc cttggaggtc gcagcgaatc actcgcttgc cgtcggcgga agcgaccaag
     8341 gcgcgcagcc gaagtctttt ttcaaggtat tccgcatgag ccgccagcgg caaggtgcag
     8401 ctcccgccaa gagcacggct caccgcccgc tcggcgcgca cgcagatcgt cgtcgcgcgg
     8461 tcggcgagcg gtgcgagcag cgcagcgacc tcggcgccgt ctgcccggca ctcgatgccg
     8521 agagcgccct gtccgggcgc cggcagcatc tcgtcgacct ccaggcgcgc ggtgatgcgc
     8581 acatcgaggc cgagccgcgt aaggcccgcg gcggcaagca cgatcgcgtc gtactcgccg
     8641 cgatcgagct ttgaaatccg cgtgtcgacg ttgccgcgga gaaggcggat ctccagacgg
     8701 ggaaagcgct cggagatctg cgacgagcgg cgcaggctgg aagtgccgac ggtggcgccc
     8761 tcgggcatct ctcgaagaga cttgaatttt ttcgagacca gcacatcgcg cgggtcctcc
     8821 cgcgcggtga tcgcggcgag ggcgaagccc gccggcaggt ctgcaggcac gtccttgatc
     8881 gagtgcacgg cgaggtccgc gcgcccctcg gccatcgcgg tctcgagctc cttgacgaac
     8941 aggcccttgc cgcccgcctg gtcgagccgc cggtcgataa gctcgtcgcc gcgcgtgctg
     9001 aggggcagca attccacctt caatccggcg tggaatctct tcaacaacgt cgcgatgtgc
     9061 tccgcctgcc acagcgcgag gcggctgcgc cgcgtcgcga tcaccagctt cttcatgtca
     9121 cgagatcgcg cagcagcggc cattgccgcc ggcttaccgg caggcgctcg tccaggcctt
     9181 cgagcaccac cagccagtgc gcctcttcgt cttcgccggg cgcgcgctcg aacccgcgga
     9241 tcgcggcgcg tgcgaccagg cagttgcggt gaattcggac gaagcgcacg gcgaactcgc
     9301 gctcgagcgc gaccagcgac tcttcgacca ggtgctcgcg gtcgcgtgtg cgcagcgtga
     9361 cgtatttctg ctcggcgcgc aggaacagga tgtcgcgcac cggcacgagc acgatgcggc
     9421 tgcgttccgt cacggagaga tactcgcgcg gcgcctcagc cgcctttgca agtcgctctt
     9481 gcggcggaac ggaggctttt tgcagcgccg cagccaggcg ctcggcacgc acgggcttca
     9541 gcaggtagtc gagcgcgttg agctcgaacg cctcgaccgc gtggcgctcg tgcgcggtga
     9601 cgaatacgat gcgcggcgcg cgctcgagcg cgccgagatg gcgcgcgacc tcgagcccgc
     9661 ccatgcccgg catcttgatg tcgagcagca cgacctcggc cccgctcgcc ggcacccgct
     9721 cgaccgcttc cacgccgtgt cgcgcctcgc cgaccacgct ggtcgccacc tcggccgcga
     9781 tgtcgccgag tagcgccctc agccgctcgc gcgccggcgc ctcgtcatcc acgatgaaga
     9841 ccttgagggt catgccatcc tgatcttgta agggatctcg atctcgacac gatagcgccc
     9901 gtccgcggcc acggtcgtaa tgcgcgcctc ggcgtcgaag aagagctgca gccgctcgcg
     9961 gatgttctcg agcgccatgc ggttgccggc acgcccggcc tcggcttcga gaaacggatt
    10021 ctcgatcagc gcgagcacgc ggttgccgcg ccgctcgatg cgcaccagca cttcgccggc
    10081 tcccgtgccc ggctccacgc cgtggtacac cgcgttctcg aggagcggct gcagcaccag
    10141 cggcggcagc agggcatccg ggggcgcgcc gtcgagctcc caggtgacgc gcagccgctc
    10201 gccgaggcga agctgctcga gcccggcata gcgctcgagg agcgagatct cgtcggcgag
    10261 tcgcacgaac gcacgcgggt ccgacatcag cgtgcgaaag agatccgcca gatcctcgag
    10321 agcgcgctcg gcgcgctgcg ggtcgcggcg gatcagggcg agcaccgcgt tcaggctgtt
    10381 gaacaggaaa tgcggccgga tgcgcgcttg cagcgcctgc agccgggctt cggcaagcgc
    10441 cggcgaatgc gccttggaaa gaagacgcag gtatgccagc agtgcggcgg tgatcagcac
    10501 ggtgagaatc agcacgcgcg cgagcgcctc ggtcgcgccg atcgtcgcga ggtagccggc
    10561 gacgactgcc gcgacgagcg ccacgaccgc cacgcagccc gcccagtaag gcagccggcg
    10621 cagcgttccg gaaagcgcga acagcacggc gatgatcact agcagcagcg gttcaaggag
    10681 cgcggcggcc tgcacgaagc gatcgagggc ctgtgcaaaa gtcgccgtgt tgaacaccag
    10741 acccgcgaac acggccgcat tcaccgcaag caaaacgcgg gcgatgatcc ccaaattgcg
    10801 gaaatccggc acaaggtcca aataggtatt ttgccttata ctgaccatgg ttacgaaggg
    10861 gtcttcaacc gtattcttat gacaagaaag ccctcgcctt ggtccgcgcg tttctccgag
    10921 cccgtcgacg agcgggtcaa gcgctttacc gcgtcggtct ccttcgacca gcgactggcc
    10981 aagtatgaca tccaggcgtc gcgcgcccac gcccgcatgc tcgccgcgca gcgcatcctc
    11041 acccgccgcg acctggcggc gatccagcgc gggctggcgc gcatcgagcg ggaaatcgcc
    11101 gccggacgct tccgctggtc gctggaggcc gaggacgtgc atctgaacgt ggaacggcgc
    11161 cttacgaagc tggtgggaga cgcgggcaag cgcctgcaca ccgcgcgctc gcgcaacgac
    11221 caggtggcta ccgacctgcg gctgtggctg cgcgcggaga tcgacgagct gcgcgccctg
    11281 atcgcagcgc tcgagcacgc gctcctcgac caggccgccc gccatgccgc gctcgtcatg
    11341 ccgggctaca cgcacctgca ggtggcgcag cccgtgacct tcggccacca cctgctcgcc
    11401 tacgtggaga tgttggagag ggataggcag aggctgctcg aagcgcgcgc aagggtgaac
    11461 tgcttgcccc tcggcgcggc cgccctcgcc ggaacgacct tcccgatcga ccgccggcgc
    11521 gtggcgcgcg agctcggctt cgaggcgctg tgcgagaact cgatggacgc ggtctcggac
    11581 cgcgacttcg cggtggagtt ctgcgcctgc gccgcgctcg cgatggtgca tctatcgcgg
    11641 ctcgccgagg agctcgtgct ctgggcgagc ccgcgcttcg gcttcgtgcg cctgcccgag
    11701 gcgtattcca ccggctcgtc gatcatgccg cagaagagga accccgacgt gcccgagctg
    11761 gtgcgcggca agaccgggcg cgtggtcggc gcgctggtgg cgctcctcac tctgatgaag
    11821 gcccagccgc tcgcctacaa caaggacaac caggaagaca aggaggcgct gttcggcgcg
    11881 gtcgatacgc tgaaggactg cgtttccatc ttcatcgggc tggtggaagg cctcgagccg
    11941 ctgccgcgcg ccatgcgcgc cgcggtgctc gagggccacg ccaccgcgac cgacctcgcc
    12001 gactatctgg tgcgcaaggg cgtgccgttt cgcgacgcgc acggcgtggt ggcccgcgcg
    12061 gtgcgggagg ccgagcgcgc gggcgtcgat ctcgcttcgc taccaataaa gaagttaaaa
    12121 gaattttcag caagaatcga cgcggacgtc gcgaaagtgc tcaccccgga aggctcggtg
    12181 gcggcgcgca agcatccggg cggcacggcg ccggtcgcgg tgcgcagggc agtcgcgcgc
    12241 gcccgcaagc gcctgcggaa atgagccgtc gggagatgct cgcgcagatc ggaaagtacc
    12301 cggtcttgcg caagctcggc gaaggggcga cctccgaggt ctacctgtgc agcgacccgt
    12361 tcaacctgcg cgacgtcgcg atcaaggtgg cgttcccgga gtcgttccag gacccggcac
    12421 gcggcaaggt gtaccgcaag ctgttcctca ccgaggccaa gctcgccggc aagctggtgc
    12481 acccgcacat ctgccagatc tacgatgcgg tcgcggaaga gaagctgcac tacctcgtga
    12541 tggagtacgt cgacggcggc acgctcgaga agtactgtaa cgggcaggcg ctgcttccgg
    12601 tcgagcgcac ggtcgagatc atcttcaagt gcacgcgcgc gctcgagttc gcgcacaagc
    12661 tcggcatcac gcaccgcgac atcaagcccg ccaacatcct gctcaccggc gagaccggcg
    12721 tgaagatcac cgacttcggc accgcgctga tcgccacggc cgagacgacg caggtggcgg
    12781 gcgtcggctc gccggcgtac atgtcgcccg agcaggtgaa ggatctgccg ctcgatcatc
    12841 gcaccgacat ctattcggtc ggcgtggtca tgtaccacct gctcaccggc cggctgccgt
    12901 tccaggcggc gaacaattac acgctgatct accagatcac caacgccgag cccgacgcgt
    12961 ctaccagctt gcggcgggac atcccgccgg cagtcgacgc catcgtgcgg cgcgcgatgg
    13021 ccaaggacct caacaagcgc tatgcgcact ggggcgactt ctccgacgat ctcggcgccg
    13081 cgttccgctc cgagcatttc ggcgcgcgca aggtccagtc gttcgccgac tccgaccgct
    13141 tcgagaccct gcgccggctg cagttcttcg agcgcttctc cgacgccgag ctctgggagg
    13201 tggcaagtat ctccacctgg cgccacgccc cggccggcga gatcctgatg aaggaaggcg
    13261 agcccggcga ctacttctgc atcctcgccc agggcgaggt gaagatcacc cggcacggca
    13321 agctcctcaa cgtgctgcgc gccggcgagc cgttcggcga gatggcctac ctttcccgca
    13381 acgagcatgc gcgcggcgcc gacgtgaccg tgtccgccga cgccagcatc atctcggtgc
    13441 ccaccaaccg gctgaaccag gcctccgagg gctgccgcca caagttcgac cgcgccttca
    13501 tggagatcct ggtcgagcgc ctcacgatgg ccaacctgcg ggtctccggg gtctagagaa
    13561 cgagattcgg ctaaggatcc ggccctggac cacgtcgcca actacccgat tatccgcaag
    13621 ctcggcgaag gcgcgacctc ggaagtgttc ctgtgccagg acccgttccg cggccgcgag
    13681 gtcgcggtga agcgcatttt cccggagtcg ctgcgcgacc cggcgcgcgg tcgcctcttc
    13741 aggaagctgt tcttcaccga ggcttcgctc gccggcaagc tcgatcaccc gcacatcgcg
    13801 cagatctacg acgccggcat cggcgaggac tcgggctaca tcgtgatgga gtacgtcccg
    13861 ggcgggacga tcgagcgctt ctgcgcgccg gacaacctgc tgccggtcga gcagaccgtc
    13921 gagatcatct tcaagtgcgc ccgcgcgctc gcctacgcgc accagcaggg cgtgacgcat
    13981 cgcgatatca agcccggcaa cgtgctctac gctgccgcgc cgaccgacgt gcgcatcagc
    14041 gacttcggcc tcgcgctcaa cgtcggcgcc gagaccaccc agatcaccgg cgtcggctcg
    14101 ccggcgtaca tgtctccgga gcagatccgc gaggagctgg tcgatcaccg caccgacgtc
    14161 tactcgctcg gcgtggtgat gtaccagatg ctctgcggca agctgccgtt ccagggctcg
    14221 aacaagttca gcatgatcta ccagatcacg cagtccgacc cgccgccgcc gtctgcgcac
    14281 cggcccaagg tgcccgaggt gctcgaccgc atcacgcgcc gcgcgatgca caagaccacc
    14341 cgggcgcgct acgcgagcgc cgacgagctc gctgccgatc tggtcgaggc gctgcgcacg
    14401 ggtctcggcc ggacacggcg caaccggttc ggcgacgcgg acaagttcgc tgcgctgcgc
    14461 gcgatgcagt tcttcaagcg cttcaccgag cccgagctgt gggaagtggt gcgcgtgggc
    14521 gaatgggagc gggtgcccgc cggcaccgag atcgtgcgcg agggcgaccc gggcgacttc
    14581 ttctgcgtgc tggtggaggg cgaagcgcgc gtcaccaaga accgcaagct cctcaccgtg
    14641 ctcgggcagg gagagtgctt cggcgaaatg gcgtacctgt cggccgaccg gcgcgagcgc
    14701 ggcgcgaccg tgactgcttc caaggacgcg catatcgtgc gcgtacgcac cgccgagctc
    14761 gagcaggcct cggtcaactg ccgcagccac ttcgaccgcg cttttatcgg tatcctcgtg
    14821 gaacgcctca acctcgccaa cacacgcctc acctccgggt aggccagcat gcgtttcgga
    14881 gcactcttta tcttgtttgt ggcttgcggt gtgctgcgcg ctgcggagca tgaggagcgc
    14941 gaccgcgccc tgctgagcgt ggcccagggc tgcgcacgcc agatccccgg cgcgctcgtc
    15001 accggcgtcc gcaacggcgt tgtccagata aagctgatga accaaggtga cggggcgggc
    15061 ttcgaccgct gctaccaaga agcggccccg cgcacgctcc aggccctggc gttgggccgg
    15121 ctggccgagc acgcagccca caccagtgtg gcgctcgaga ccgcaggctc gctgatgttc
    15181 gtgcccgcgc tggtcaacgg cgtgcaggtg cgcctgcttc tcgataccgg agccggcaag
    15241 acgatcgttc ggccgctcat cgcgcagctc gccggcatcg agcccggccg ccaagcgccg
    15301 ctcgcgcaga tcaccgtcgc gggcggcgga cagctctcgg tgccgctggc aaaggcgcgg
    15361 tcgctggcga tcaacgaggc cgcagtgctc gcgattgaaa tcggcatcta tgaagcgctg
    15421 cccgacctgc ccgacgtgga cggcatcctg gggagcgact acctgagcca tttcacggtg
    15481 acgatcgacc gccagaaggg cacgctcctg ctcgtgcccg cgcgtcgtca agttcgctcc
    15541 gattgagccc ggcatgcacg ccgcgctgcg caagcagctg ggcatcgccg ccgcagaacc
    15601 tgtctccggc ggctgcattc accgctgcta tcgggtagcg attgccggca cccagcggtt
    15661 cctcaaggtc aacagcggcg cgcatgagga tgcgttcgcc tcggaagccg atggtctcgt
    15721 agccctgcgc aatgccggac tgcacgcgcc cgagccgctc gcgcacggcg tcgccggcga
    15781 cgaggcctac ctgctgctcg aatacctgga gctaaaaacc aagggcgact tccccgcgct
    15841 gggacggatg ctcgccaatg cgcatcgaaa acccgggccg cgtttcggct ggcaccgcaa
    15901 caactacatc ggctccaccc cgcagcagaa cggctggtgc gacgactggg ccgagttctg
    15961 gatcacgcga aggatgaagc cgcagctcgc gctggcgcga cgcaacggtt ttgatctcga
    16021 agtgaagatc gatctgggaa accaccgccc ccaaccgtcg ctactgcacg gcgacctgtg
    16081 gagcggaaac gcgggctttg cggcgcaggg gccggtcgtg ttcgacccag cggtgtacta
    16141 cggcgatcgc gaggccgacc tcgcgatgac cgagctgttc gggggattcc cgcgggagtt
    16201 ctacaaggcc tacgacgagg cgttcccgtt accggccggg tacgagaagc ggaagcacct
    16261 gtacaacctc tatcacctgc tcaaccacct caaccttttc gggggtggct acctcagcca
    16321 ggtgaagtcg accctaggcc ttctcggtct ctaggagctt ctgcagctcg ccggactggt
    16381 acatctcgcg catgatgtcc gagccgccga cgaactcgcc gttgatgtag agctgcggga
    16441 tcgtcggcca gttggcgtat tgcttgatgc cttcgcggat ttcggcgtcg gccagaacgt
    16501 tgacgctgtg gaaatccttg acgccgcagg cgctgagtac ctggacggcg agggccgaga
    16561 agccgcattg cgggccctgc ggggtgccct tcatgaaaag cacgacgcgg ttgccggtta
    16621 cctgctcctt gatgcgctgt tgagtatcca tggggtgcat tttatcgctt tccgccgctg
    16681 actcgcggga ttccagccag atcgggccag cttgcggtgg tttcaagccc cgcggcctcg
    16741 agcagtgcgc ggaccgcttc gtgctgccct gcgccatgct cgagcaggag ccagccgccg
    16801 ggcaacagat ggtccggggc cccgcgcgcg agcatccgga agccatcgag gccgtcggcg
    16861 ccggcgacga tggcgaagcg cggctcgtag tgcaggtcga cgagatgcgg gtcgtccgcg
    16921 acgacgtagg gcggattgca gatgatggct tcgaagcgct cgcccgcgag cggctcgaac
    16981 cagcggccgt gacgcagctc gacgtcgagg gcgaggcgca cggcatttcg cttcgccacc
    17041 tcgagcgctg aggcgctcgc gtcggtggcc accacgcgcg cggcagacag atggcgcttg
    17101 agggcgagcg cgatcgcccc cgagccggta ccgatgtcca ccaccgaggc gggcttgcgc
    17161 gcgagcgcca ggtcgacgag gagctcggtc tccgggcgcg gaatcagcac cgccggcgtc
    17221 acgaccagat cgagcccgta gaactccttg cggccgagga tgtaggccac cggctcgccg
    17281 gcggcccgcc tcgccgtcat ttgggaaaac gaggcctcga cgtcggacgg caactgccgt
    17341 tccggatggg caagaacgct ggcttccgaa aagccggtcg ccgccgcgag cagcagccgc
    17401 gcctcgcgcc catcgatgcc gctcgcactg agtgcttcta cgattttcaa gcggcttctt
    17461 cgcgtccttc ggtcaagcgg cttcttcggt gaggctggcg agctgctcgg cctggtgctc
    17521 gtgcgcgagc gcgtcgatga tctcgtcgag ctcgccgtcc atgatgcgct cgatcttgta
    17581 cagcgtcaga ttgatgcgat ggtcggtgac gcggccctgc gggaagttgt acgtgcggat
    17641 gcgctcggag cggtcgcccg agccgatcag cgacttgcgc gtcgaagcgg tcttcgcctg
    17701 ctgctcgcgc tcctgcttgt cgcggatctt cgccttgagc acggagagcg ccttctcgcg
    17761 gttcttgtgc tgcgagcgcg agtcctggca ctcgaccacg atacccgtcg gcagatgcgt
    17821 gacgcggatc gccgaatcgg tcttgttgac atgctggccg ccggcgccgg aagcgcgaaa
    17881 agtgtcaacg cgcagctcgg cggggttgag caccacatct tcgatcgcgt cggcttcggg
    17941 caggaccgcg acggtgcacg ccgacgtatg gatgcggccc tgcgcttcgg tcttcgggac
    18001 gcgctgcacg cggtgaccgc cggactcgaa cttcaggcgc gagaacgcgc cctggccgac
    18061 gatgcgcgcg atcacctcct tgtacccgcc gagctcggag gcgctctcgg agatgatctc
    18121 cacctgccag cctttgcgct cggcaaagcg cgtgtacatg cgaaagagat ccccggcgaa
    18181 cagcgccgat tcgtcgccgc cggtgccggc gcgcacctcg atgaagaggt tgcgctcgtc
    18241 gttcgggtcc ttgggcagca gcgctttctg cagctcggcc gcgagccgct ccatcgcggc
    18301 gcgcgccttc ttcagctcct cgtcggcgta ggccttcatc gtcgcgtcgg acgcaaggtc
    18361 gcgcgcctcg tccgcttcgc gctccgcctg caagtagctt tgaaaaagag aaaccacgga
    18421 agaaacttcg gaatgctcgc gcgagaggcg cttgaactgc tccatgtcgc gcgtcgcgtt
    18481 ctcggcggcg agcaggcgct cgagatcgcc cagccgcccg acgaggctgt cgagcttcgt
    18541 ccgcagccgc ggattcactc gtcgcgacta gagtttcgag gcgggcgcga gaaggtaaaa
    18601 gtgagcgcct cggtcggaac gtgcatcagc ttgttggtga gggtccgcga cagcgcttcc
    18661 accaccttgg ccgggtcctc gccgcgcgcg agcgacttga gcgcgcgctc gagctcctgg
    18721 cggcgcgtgt cgtcggcttc ctcgcgcagc gcgcggatca gcggcacgcc ctcgcgcgcc
    18781 tgcacccagt gcatgaagcg accgacctgc gtgtcgatga tcgcttcggc ctgatcgagg
    18841 gcggcgcgac gcgagtcgag attggcgctc acgatgctgg cgaggtcgtc gacggtatag
    18901 aggaacacgt cgtcgagctc gcccgcctcc tgctcgatgt cgcgcggcac ggcgaggtcg
    18961 accatgaaca tcggccggcg ccggcgcgca cgcagcgcgc gctcgaccag gcccttgccg
    19021 aggatcggaa gcgacgaggc ggtgcacgac acgatgatgt cgtagtcctg caggtgctcg
    19081 ccgagcgcgc gcagctcgat cgcgcgcgcg ttgaagcgcg cggcgagccg ctcggcgcgc
    19141 tcgagcgtgc ggttggcgac ggtgatgcgc gccggcgcct gcgccgcaaa gtgcgtcgcc
    19201 gtcagctcga tcatctcgcc tgcgccgatg aggagcacgc tctggtcctt gaggctcgga
    19261 aaaatccgcg ccgcgagctt caccgccgcg gcggccatgg atacgctggc ggcgccgatg
    19321 cgcgtggtgg agcgcacttc cttggccacc gcgaacgagc gctggaacag ccgatgcagc
    19381 aggctgccga gggttccggc cgactcggcg gcacgcgccg cctctttcat ctggccgagg
    19441 atctggggct cgccgatcac catcgagtcg agacccgaag cgacccggaa ggcgtggcgc
    19501 acggcctgct cctgcggcag ggcgtagagg tagggctgca gctccccccg ggcgaggcgg
    19561 tggtactgcg cgagccactc ggtcagctca ccgggctttt ctccggaaac gtagatctcg
    19621 gtgcggttgc acgtcgaaag gatggcggcc tcgttggcga ggccgcgctt gagctcgccg
    19681 agcgcttcgt gcaggcgctc gacgtgaaat accacgcgct cgcgcacggc gagcggcgca
    19741 gtctggtggt tcagcccaag agcgaagagc gaggcattca agcccgctga ttataggaga
    19801 gtttcgaccc ccggccgcta tcggcggcgc gaatggctat gcgtgaatcc gatcgcagcg
    19861 cagactgctt tcgtcgaagt catttccagg aaaggccgat gctcgacacc gtgtcgacaa
    19921 atgcctcgac gcgcaccggc tttacgagat agcggtcgac gccgagccgg tagctctcca
    19981 gcacgtcgcg ctcctcgctg gaggaggtca ttaccaccac cgggatcgct ctgagctccg
    20041 agcccttgac ccgacgcagc acctcgatgc cgtccacctt gggcatcttg atgtcgagca
    20101 gcacgagctt cggcgggtgg cggggactgc ggccggcata cgcgccgctg cagaaaaggt
    20161 agtcgagcgc ctccgcgccg tcctttaccc aatgcacgtg atcggcgagg ctgcgcttgc
    20221 ggagcgcgcc gagcgtcgtc tcggcatcct ccgggtcgtc ctccaccagc aggatctcca
    20281 cctgttcgaa gccgctcacg ccgcccccct cggccgtcgg tgaagctagc gcgaatcccg
    20341 ctcgctgtgt gtcaggatgt ttgtgatcag gcgctggtga gaccgcgccg gatggcgaac
    20401 ttcaccagcg ccgtgaggtg atcgacgtcg agcttcgtca tcagccggct gcggtaggtg
    20461 tcgatgctct tcggcgacag gccgagcaac tgcgcgacct cgtggctggt gttgccttcg
    20521 accaccagct tgagcacttc catctcgcgc gcgctcagtc gctccagcgg atcctctacc
    20581 acggcctcgc cagagccgcg cagcgcgatc cccgcgctca ggtagcgctt gccggcgtgc
    20641 accgcgcgca cggcggccac gacctcggcg cccgccgcct ccttgagcac gtaaccgttg
    20701 gccccggccc ggaacgcctg ctggacgtac tccggccgcg cgtgcatcga cagcatgagg
    20761 agccgcgtct tgggcgacac cgcgcgcagc cgccgcgcca ggtcgatgcc gctcgtccgc
    20821 cgcatggcga cgcccacgat ggcgacgtcg ggctcggcac gcgccgcgaa gcggaccgcg
    20881 gccgtgtcgt cgtcgaagga gccgatgacc cgcaggtccg gcgcttcctc cagcagccgg
    20941 gtgacgcctt cgcgcaccgg ggcgtggtcg tcgacgatga cgattcggat catgcggaca
    21001 cgatacggcg cggcgcgccg cagcactttc caggggtgtg aaaacacggg aaggattcga
    21061 ccgggcggtg tgcgtttacg cacaccttcc gggcaatact ccccgaatcg ggcgattgcg
    21121 ccccacgcac gtcccccgca accaccgtca gggcggcacc ccgacaccac cgcctcacct
    21181 aggcgcacgc ggcaagagaa acctttcatt gtttcccacg ggtaggccct tgatgaagca
    21241 ggcgccgcgc gtgcactttg tgcacgccgc cggatcaccg gcgggcagat ccctgaagtt
    21301 tctgacccaa cgaggagaag cccatgtcca gcaagaagca gaaccagtct gtcgtccgca
    21361 agcccgcgaa aaaactcacc gcaccgcgcg tcgtgcgcgc gcaagactcc gccacccgcg
    21421 actcgggcac gatccgcttc ggaagcggct ccgcgccggc cagcctccgg aagtgaggca
    21481 ctcttcccat gaatgaagca cgccagccga accccgcggt cacgttttac ggggcgatac
    21541 cgggttgtcg cgcgccgatg cgcgccgatc cctcggtact cgggacgctg cccgcacgag
    21601 gcttccagta ttgcgaagcg ctgagggcgg cttcgtcctt cggctggtac gctttttcgc
    21661 cgatcgactt cagccttctc tgggatgggt cggaggtcat ctggacttac cgcggcgcta
    21721 aagcgtggta tccgctgaaa agcgcgcaat tccccggctt tagccgctac ttcgacgaca
    21781 cggcgcccaa gcggctgcgc ggcttcgcgc cgccgttcct cacctcgacc tgggatcctt
    21841 cgctgatcca ggtctggaca ggactcttcg tggagagcgc gccggactgg agcattctcg
    21901 tgcgcccccc ggccaacctg cagcgccacg agggctacca cctgtacgag gggatcgtcg
    21961 agtccgaccg ctggttcggc ccgctcttca ccaacctgcg gctcgtcaag accgacgtgc
    22021 cgattcactt cagcaccgag ctgccgctcg tccaggtgca gccgctgcac cgaagcgcct
    22081 acgccgagga aatctcgaac cacttcggcc tggtgcccga tctcgccgat cttccggccc
    22141 acgcgtggtc gcgctacgag gaggcgatcg tgaaaccgag catcgagccg cgcccggccg
    22201 gtgcgtatgc cgcttcggtg cgacggcggc gcaagagcgg ctgcccggtg cacgctgaat
    22261 gaatggctat atcgcggcgg ctatcgcggt actcatctgg gcgtcgtacc cggtggcgac
    22321 ccgcgccggc gtcaccggat cgttcacgcc gcaggaactg gtaacgcttc gcttcggcgg
    22381 cggagccctg cttttcctgc cgtacctcct gctgcagtgg cggcaagtca gccgctcggc
    22441 ctggctgcaa ggcgtaccgc tcacgctctt ccaaggcgcg ggaatgggag ctctggtgat
    22501 ctgcgggctg caattcgccc cggcggccca tcaggcagcc ctcggtcccg gcgtatcgcc
    22561 ggcttgggtt gcgctgcttg gcttcgtgat tttcgcgcgg cggccgtcca cgcgggcaat
    22621 cgtgggggca acgctatgcg caaccggggt gctcgcgctc gcgagctgga gcggtatggc
    22681 gcatgacgcc cgcgtgctcg cgggcgacgc gatgttcctt gccgcctcgg tgctcggcgc
    22741 gctctacgtg ctgcagttg
//
LOCUS       DATBSZ010000197        18131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_444205, whole genome shotgun sequence.
ACCESSION   DATBSZ010000197 DATBSZ010000000
VERSION     DATBSZ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18131
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444205"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1863
                     /locus_tag="VIG70_12545"
     CDS             127..1863
                     /locus_tag="VIG70_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY8251426.1"
                     /translation="MRDVPVASPTQENLWGSDAIAAMLRALDIPYLALNPGASYRGLH
                     DSIVNYLGNERPQMLLCLHEEAAVAIAHGYAKASGRMMGVVLHSNVGLMHGSMAIFNA
                     WCDRMPMLILGATGPWDAARRRPWIDWIHTASDQGALVRDYTKWDNQPASVPAAYEAL
                     LRAVQIADTAPRGPTYVNLDAALQEAKIGALPPVPDASRYRAPEAVLPSKQGVEKAAQ
                     LLSGAKSPAILVGRVSRSEAAWKARVALAEKLQAKVFTDIKTPAAFPTDHPLHAAPPA
                     TFPDGKLLRDADVVLSLDWVDTAGTLKAAWGDAPIGAKVIRVSVDAHIHRGWSMDYQG
                     LPPSDVYLMCEPDVAVPLLNDQVRSRPAAVLPKQEFPAGRDDVVSIRTLAKAFNELTE
                     GMDISLSKLPLGWNGAYRHFRHPLDYLGADGGGGVGAGPGCTVGAALALRGSGRMVVG
                     IMGDGDYLMGVTALWTATHYKLPCLILVANNRSFYNDELHQERVARERGRPVENKWIG
                     QRIDEPDIDLAAMARAQGAVGIGPVKEISQLKSSLAEGIKHVKSGAVCVVDVRVMPGY
                     DAPMSGAPPARR"
     gene            1885..3090
                     /locus_tag="VIG70_12550"
     CDS             1885..3090
                     /locus_tag="VIG70_12550"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8251427.1"
                     /translation="MASPLAYREFRSLWTGSVLSWWSQWIQHAALGWVVYEITGSGAL
                     LGAVMGVRAIPMVLLTPVSGMAADRYDRRRLMQASQALAAAVSLAFGAALALDMVSLW
                     MLFAFTMLMGASNVMDRPARFTTSFELVPRELAVKAVALNTIGFSLARVLGPAVAGYL
                     IAAVGGAGTFFLQGVLYAASGAMVLMVAFPPRAPRKETRSAFADMAEGLRFALSDPRT
                     RVLLVVGALPFFLLIPVFGTLYPIYAKDEFAAGPTGLGLLLTAVGVGGTLGGFIANAL
                     GRVQRQGVLQAAWVVVMAASIIGIAMSSSISWAIAFSVIGGAAEMAHASSNMAMLQIG
                     APEAMRGRISALLMLNPAFISLGAFIAGPLSDVAGVRNASMILAAGAIAAIAVLYLVS
                     PILRELRHQ"
     gene            3087..3815
                     /locus_tag="VIG70_12555"
     CDS             3087..3815
                     /locus_tag="VIG70_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY8251428.1"
                     /translation="MKTKLSASRALVDDVVAASRILAQHEVLDAYGHVSARSDKKPAN
                     FIMSRSLAPALVTAADLMELDADSEPLPGEKRKGFIERYIHGEIYRTRPEVMAVVHSH
                     SPSVIPFGVTRTKLRPIYHMGSFLYSGAPVFEIRKETPDNDLLIRDNRLGKALAGTLG
                     KCNCVLMRGHGMTVIGDSVPEAVFRAIYTEMNARLQLQATQLEGPIEFLSEEEGRRSM
                     ASNRGTLERPWELWKKSVSARGRP"
     gene            complement(3793..5358)
                     /locus_tag="VIG70_12560"
     CDS             complement(3793..5358)
                     /locus_tag="VIG70_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251429.1"
                     /translation="MAGAEISRNLAARRLLARLAALILVAAASAATAADKVFTYALEG
                     APESLDFAKTSTERAIRVAWLLCDALVNVSKDGQSLEPGLAESWKFSADGLSAVMKLR
                     PGVLFHDGTPLDAAAVKASIERQFRPSHPLYTAEPSNVQAQVLAELVQDIQVLDARTL
                     TLKLKYPGLHYLSQSEIVSPTALRKLGKDFGRNPVCSGPFKFESWTKEQIVLVANDQY
                     WAGRPQIDRVVFRFIAEGKAVVDALLKGEVDFVARLPDPVWFERLRESPRIKSVPFPG
                     LNLYYLGFYTERPPFNNAIVRRAVAQAINAPRAALFLGRGVAVPAKGSLPPGVRGYDP
                     EVGQGPYDPQAAKELLNKSGPTALPTVRVVHNSALTFTAEIAGAIQSDLRRIGIQVEL
                     LGKPGWPEVVAAVRKREGDMFLYSWNVRAPYPERFLLPLFHSRSAGTTNLMHYRSPGL
                     DKILDEALRMPEGPAQNRMLSQAQRMIVEDAPMVFLYHAIRVAAYADRVQGLELNLGL
                     LPHDKLVKVDLAR"
     gene            complement(5345..5818)
                     /locus_tag="VIG70_12565"
     CDS             complement(5345..5818)
                     /locus_tag="VIG70_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251430.1"
                     /translation="MASRGQYLLRIGGRLEGNLKEKDVLSVLGQVFRTPRGGEGVPGE
                     RDTGGWPEGFVLMNYSPCQWDLKVDYPHPDQTMISFRAEIHHTFPNSLYQNRAEMLRH
                     VEWFYHYREYRWWKELTKHLEKDVNPALRKKFKQDCMPERLWFRDWQWYCGNGGC"
     gene            6026..8458
                     /locus_tag="VIG70_12570"
     CDS             6026..8458
                     /locus_tag="VIG70_12570"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8251431.1"
                     /translation="MGSLLHRYRNLSLRAKFALPIALFVVLLFGALIPGVLYLQTRTV
                     LDGARERGLELTKIFAHSSVQAVVADDFLVLRQMINSIATEQDVLYAMILDPNGRLLV
                     HSDMRQAGRTYTDAPSRRAMETTEPLVQEAQAGTVRMYDFAVPIYVLNERRAVARVGI
                     SLEREWAGIRRTRNLVLGLGVLALAMGLGLASWLARSVTRPVRELAEGAQEIAAGNLD
                     RVIAVRQMDEVGRLGETFNRMAGSLKARWEIDRDLSSTLDLESVLETITRHARGLLGA
                     DIACVATVNPATKGATIVASAGAQTDALRNLRVAAGRGLGGEVLSTGRAVSVADCAKD
                     PRIADEHGDLLRREGIVSALVVPISLKGRTTGLLYVANRRATAFSRQDEDTLNHLAAQ
                     AAIAIENARLYAEVTRHAEDLEGKVEERTRELQETNRRLETASRHKSEFLANMSHELR
                     TPLNAIIGFTRLVMRRSKEVLPVKQYENLEKIHLSADQLLLLINDVLDLSKIEAGRMD
                     VRPIAFNLESLIDVCLRTVEPMVKSEQIQLAKDLEPDLPPLVTDQDKLKQILMNLLSN
                     AIKFTEAGTITVSARRHGDRVAVAVTDPGIGIPEEALDLIFEEFRQVDSSSTRRHGGT
                     GLGLSISRHFARLLGGNVTVASRLGRGSTFTLTIPMRYALDEPAPQPGAAALKPGEPT
                     HNGKVMLAIDDDPDTIYLLRENLIEAGYHVVGALSGEEGLEKARTLRPFAITLDLLMA
                     HRNGWDVLRDLKSDPITMGIPVIVISVVDSKEPAYRLGAFDYLVKPFDRDAILRALER
                     IPPPGRGRAAVG"
     gene            8460..9911
                     /locus_tag="VIG70_12575"
     CDS             8460..9911
                     /locus_tag="VIG70_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8251432.1"
                     /translation="MKKVLVIEDVELNRELVAQLLEEDYQVLVAGDGESGIRLAARER
                     PDLILMDLSLPIMDGWEAARRIKAHPDLKHIPIIAVSAHAMRGDAEKARKSGCDDYLS
                     KPLDENLLRDKLQKYLGPLYRRSGARREEGTPRILIVDDEPLNVDYLQQELEELGYDT
                     LSARDGQEALECVATDPPDLILLDILMPVMDGFAVCRALKGDEATRLIPIVIMTTLDG
                     IDDRIRGIEAGADDFLTKPVNQRQLIARLQTTLRLKHTVDRKLRELRQIKDHFAKFVP
                     EAVKRLVAENPEAPELDKRERDVSVLFVDISGYTRLSERLNAPTLNAVVERYFSSFLD
                     RIHEGGGDINETAGDGFMAIFQDPDIDTHAIKAVETALAVQADTEALNRESNDPPLAI
                     HIGVNSGATLVGSTRFEGLRGARWTFTATGNVTNLAARLAAAAESGQIIAGPETVRRL
                     GSRYQLERLAVERLKNIAADIPLHRVLGPSAAR"
     gene            complement(9912..11075)
                     /locus_tag="VIG70_12580"
     CDS             complement(9912..11075)
                     /locus_tag="VIG70_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8251433.1"
                     /translation="MTLGGSGMYSIAVALAPVQAEFGVTRGDASLPYTLTMLGFGIGG
                     ILMGRIADRFGVVVPVVIGALGLGSGFVLAGTAGSLLQFSLAHGLMIGFLGCSTTFAP
                     LVADTSLWFNRRRGIAVAICASGNYLAGAVWPPILQYSFDTVGWRSTYVGLGIFCAAA
                     MLLLALAMRRRPPALDISSQAIQARSFGERPLGLSPNALMVLLVIAGVGCCVAMSMPQ
                     VHIVAYCGDLGYGAAQGAQMLSIMLAFGIVSRLVSGWICDRIGGLRTLLLGSMLQGIA
                     LVMFLPFDGLVSLYVVSAMFGLFQGGIVPSYAIIVREYFPPREAGVRTGIVLMATLFG
                     MAFGGWVSGAIFDMTGSYRAAFINGIGFNLLNLTIAFWLLRRSLPGRAVAAAG"
     gene            11181..11510
                     /locus_tag="VIG70_12585"
     CDS             11181..11510
                     /locus_tag="VIG70_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY8251434.1"
                     /translation="MAARQPPSTIAQVRDVFTKLGEISDKVVFGDVWERKQLSKRDRS
                     LITVAALTALYRPDQLRGHLHRAFDNGVTKEEIIEVITHLSFYAGWPNGGTAALIAKE
                     VFKERGV"
     gene            11507..12388
                     /locus_tag="VIG70_12590"
     CDS             11507..12388
                     /locus_tag="VIG70_12590"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8251435.1"
                     /translation="MKIGFIGLGAMGRPMAASLQRAGHALQVYDLRRVEGFPNWKDSA
                     AKAAEACELLFTSLPGPAEVEAVAAHLESPVVWFDLSTNSPKVIRRLHAGLQKKRIEL
                     LDAPVSGGPKGAQSGKLAIWVGGEQAVFEKFKPVLQAIGDQVLYVGSIGAGTVAKLAH
                     NAASFTIQAALAEVFTLGVKAGVEPLALLHALRQGATGRKRTFDRLAEHFLPGVYDPP
                     AFTVRLAQKDVALALELAKECGVPMRVGKIALDELDEAIKRGWGERDCRVAMTLQEER
                     AAVSVRVPRERLENLPD"
     gene            12397..13185
                     /locus_tag="VIG70_12595"
     CDS             12397..13185
                     /locus_tag="VIG70_12595"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY8251436.1"
                     /translation="MGRGRLVMAVIIALMAIITYFSSTSENPLTGEKQRVAMTPEQEI
                     ALGYKSAPQMAAQMGGLSQNEKAGALVKQVGEKLVAESVAAKSPYKFSFHVLADPRTV
                     NAFALPGGPIFITEGLLRLLKSEAELAGVLGHEIGHVIARHSSERLAKQQLTQGLLGA
                     LVVGSGDYSTAQIGQVVGSMINMSYGREDELESDALGIRIMAEARYDPRAMIRVMEVL
                     AKASGGSRQPEFVSTHPAPENRSARIKAEIEKRFPQGVPKGLKE"
     gene            complement(13155..13850)
                     /locus_tag="VIG70_12600"
     CDS             complement(13155..13850)
                     /locus_tag="VIG70_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251437.1"
                     /translation="MLSLAEPPIVAKVRRRLRRHGLTATLHAYGMRAVNSAVSFRILR
                     AMCLAQVDPSFLGCPAGYTASFASREALYGFVEQPGSELSLKFVQQALSRGDQCFAIC
                     DGPSLVAYGWYAFRPTPTHLPETLLHFDPRWVYRYKGFTLAPYRGRRLHAFGTTLALR
                     HYLARGFCGFVAYVASTNFDSLKACLRVGYRPFGSICVLQAFGRLATFSTPGCDRLGF
                     RLESYSLSPFGTP"
     gene            complement(14108..18118)
                     /locus_tag="VIG70_12605"
     CDS             complement(14108..18118)
                     /locus_tag="VIG70_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY8251438.1"
                     /translation="MRVGLNSGEVVVRSVGSDLHMDYSAVGQTTHLAARMEQIAEPGQ
                     ILITADGLLAAEGYVEARPIGPVQVKGLSSPVEVYEVTGAGPVRTRLQAALARGLTRF
                     TGREAAMARLRSAIGLARAGQGQVVSIVGEPGVGKSRLVHEFLSSADARDWLVLKTGP
                     NSYGPATSYLPVILLLREYFKIDSRDDHAQIRRKVGEAVRSLDRTLEPILPALLALLD
                     VPVADAEWDELNPSQRRWQTFDALRRLLLRQSEAQPLLVVFEDVHAFDAESEALLDGM
                     VESIPALPVLALATYRQEYRHKWGDRGYHSQLRIDPLSAAGAGELLKTLIGDQPTLKP
                     VADMLIQRTGGNPFFLEEGVRALIETGVIARDRESYRLVKDLKTAQVPPTVQAVLAAR
                     IDRLSADEKWLLQSAAVIGRSVPFSLLQAISEHDEDTLRGLLARLQAAEFLYETSLFP
                     ELEYAFKHALTQEVAYNALLHETRRELHGRIVEALESMPGPGLSDQIDRLAYHALRGE
                     LWTKAIAYLHEEGLKATARSASGEAVACVEQALFALRHLPESAETIAQGIDLRFDLHS
                     ALIPLGEFSRIVEVLRDAEKLADRLGDRARQGRVSASMATWYWCIGDPDRALECGRRA
                     LAIAEEIEDGALEAVSNHRVGEAHVSLGNYRQAIASFERNLELRTKAPSPKRFGMAAL
                     QSVTSRSWMAWCLASVGEFGVAKAVARESIRVAEEANHPYSLATTYSGAGQRHQMQGE
                     LAQAIPLLERALDLCRRGNFGPLYRYTAPNLGLAYVRCGRIEEGLELLERSSAQPSGM
                     HIVPFDALEATNLAEARLAAGRRAQALEAAEHGLALLRLHRQRFTEPEILRVLGDIHA
                     AGEAPNFAKAESSYREAITLASELGLRPVLARSHLHLGMLYGKAGKRAQALEQLTLAR
                     ELLGELEMRFWEEKAEAATREISGSVQSQPLFDPALPEFHANPYPFYHRLRLQDPVHQ
                     SAMGFWVVTRYSDVVTVLRDARFGREDFGPMVSAVYGEDSERVPRPMVFRDPPAHTRL
                     RSLVSKAFTTRVVQGMRPHIQEIVDRLLDRVQGAGAMDVISALAYPLPVTVICEMLGV
                     PDCDHDSMRRWSADITRSLDALGLPSDREIVRRGRAARHALGEYFRGLLPERRRNPRA
                     DLLSLLIAAEEQGDKLTEDELLATCVLIFIAGHETTVNLIGNGLLALLQHPDQLEVLK
                     ENTQLLASAVEELLRYDSPVQRTARVTKTEVELGGKTIPRHALVVAAIGAANRDPERF
                     PDPDRLDLARPDKDHIAFGFGIHFCLGAPLARIEAQIALGTLLRRLPQLELATGTPQW
                     RESSTLRGLKELPIRF"
BASE COUNT         3075 a         6002 c         6183 g         2871 t
ORIGIN      
        1 cagggcatgg atatcgcgac cggctcgccg gaagagctcg cccgcgtcat ccgcgaggac
       61 gacgcgcgct ggggcaaggt catccgcgac gccggcatca agcccgaatg agcgcaccat
      121 aggcccatgc gcgacgtccc ggtagcgtca ccaactcagg agaacctctg ggggagcgac
      181 gcgatcgcgg cgatgctgcg cgcgctcgat atcccttacc tcgcgctcaa tcccggtgcg
      241 agctaccgcg gcctgcacga cagcatcgtc aactacctcg gcaacgagcg gccgcagatg
      301 ctgctctgcc tgcacgagga ggcggcggtc gcgatcgcgc acggctacgc caaggcctcg
      361 ggccgcatga tgggcgtggt gctgcattcc aacgtcggcc tgatgcacgg ctcgatggcg
      421 atcttcaacg cttggtgcga tcgcatgccg atgctgatcc tcggcgccac cggcccgtgg
      481 gacgccgcgc gccgccgccc ctggatcgac tggatccaca ccgcctcgga ccagggcgcg
      541 ctggtgcgcg attacaccaa gtgggacaac cagcccgcct cggtgcccgc cgcctacgag
      601 gccttgctgc gcgcggtgca gatcgccgac acggcgccgc gcggcccgac ctacgtcaac
      661 ctcgacgccg cgctgcagga ggccaagatt ggcgcgctgc cgccggttcc cgacgcttct
      721 cgatatcggg ctccggaagc ggtactaccc agcaagcagg gggtggagaa ggccgcgcaa
      781 cttctatccg gcgcgaaatc gccggcgatc ctggtcgggc gagtgagccg cagcgaagcc
      841 gcatggaaag cgcgcgttgc gctcgccgag aaattgcagg cgaaggtatt cacggatatc
      901 aagactccgg ccgcgtttcc caccgaccat ccgctgcacg cggcgccgcc ggccacgttc
      961 ccggacggaa agctgttgcg cgatgcggat gtcgtcctgt cgctcgactg ggtcgacacc
     1021 gccggaacgc tcaaggcggc gtggggcgat gcgccgatcg gcgccaaggt gatccgcgta
     1081 tcggtggatg cgcacatcca tcgcggctgg agcatggact accaggggct gccgccttcg
     1141 gatgtttacc taatgtgcga gcccgacgtt gcagttccct tgttaaatga tcaggtaaga
     1201 agcaggccgg cggcggttct gccgaagcag gaatttccgg cgggcaggga cgatgtcgtg
     1261 tcgatccgca cgctggctaa agcgttcaac gagctgaccg aaggcatgga catcagcctg
     1321 tccaagctgc ccctgggctg gaacggcgcg taccggcact tccggcatcc gctcgactac
     1381 ctcggcgccg acggcggcgg cggcgtcggg gcgggaccgg gatgcaccgt cggcgcggcg
     1441 ctcgcgctgc gcggttcggg ccgcatggtg gtggggatca tgggcgacgg cgactacctc
     1501 atgggcgtca cggccctgtg gacggcgacg cattacaagc tgccgtgtct gatcctggtc
     1561 gcgaacaacc gctccttcta caacgacgag ctgcaccagg agcgcgtcgc tcgcgagcgc
     1621 ggccgaccgg tggagaacaa gtggattggg cagcgcatcg acgagcccga catcgacctc
     1681 gcggcgatgg cgcgcgcgca gggcgcggtg ggcatcgggc cggtgaagga gatttcgcaa
     1741 ctcaagtctt ccctcgcgga agggatcaag cacgtcaaga gcggcgcggt atgcgtggtc
     1801 gacgtgcgcg taatgcccgg atacgacgcg ccgatgagcg gagcaccgcc cgcaaggcgc
     1861 taaggcccgg gccggcccct tcttgtggcg tcgccgctgg cgtaccgtga gtttcgctcg
     1921 ctgtggacgg gctcggtgct ctcgtggtgg agccaatgga tccagcacgc ggcgctcggc
     1981 tgggtggtct acgagatcac cggctcgggc gcgcttctcg gcgcggtgat gggcgtgcgt
     2041 gcgatcccga tggtgctgct tacgccggtg tccgggatgg ccgccgaccg ctacgaccgc
     2101 cggcgcctca tgcaggcgag ccaggccctc gccgccgcgg tctcgcttgc cttcggcgcg
     2161 gcgctcgcgc tcgacatggt cagcctgtgg atgctctttg ccttcaccat gctgatgggc
     2221 gcctcaaacg tcatggaccg gccggcgcgc ttcaccacct cgttcgagct cgttccgcgc
     2281 gagcttgcgg tgaaggcagt cgcgctgaat acgatcggct tttccctcgc gcgcgtgctc
     2341 ggcccggcgg tggccggata tctcatcgcc gcggtcggag gcgccggcac gttctttctt
     2401 caaggcgtcc tgtatgccgc ctccggcgca atggtgctga tggtcgcgtt cccgccgaga
     2461 gctccccgaa aggagacgcg gtccgcattc gccgacatgg cggaaggcct gcgcttcgcg
     2521 ctatcggacc cgcgtacccg cgttcttctc gtggtcggcg cgctgccgtt cttcctcctg
     2581 atcccggtgt tcgggacgct ctatccgatc tatgccaagg acgagttcgc cgccgggccc
     2641 accggcttgg ggctgctcct caccgcggtc ggggtcggcg gcacgctcgg cggtttcatc
     2701 gccaacgcgc tcggccgcgt ccagcgccag ggcgttctcc aggccgcatg ggtggtggtg
     2761 atggccgcgt cgatcattgg tatcgccatg agctcgtcga tttcctgggc gatcgccttt
     2821 agcgtcattg gtggcgcggc ggagatggcg cacgcgtcga gcaacatggc gatgctgcag
     2881 atcggcgccc ccgaagcgat gcgcggacgc atctcggcgc tgctgatgct caaccccgcc
     2941 ttcatctcgc tgggcgcctt catcgccgga ccgctctccg acgtggccgg tgtgcgcaac
     3001 gcctccatga tcctcgccgc gggcgccatc gccgcgatcg ccgtgcttta cctcgtctcg
     3061 cccatcctga gagaactccg acaccaatga agaccaagct ctccgccagc cgcgcgctcg
     3121 tcgacgacgt cgtcgccgcg agccgcatcc ttgcccagca cgaggtgctc gacgcctacg
     3181 gccacgtcag cgcgcgcagc gacaagaagc ccgcgaactt catcatgtcg cgctcgctcg
     3241 ccccggccct ggtgaccgcc gcggacctga tggagctcga cgcggacagc gagccgctgc
     3301 cgggcgagaa gcgcaaggga ttcatcgagc gctacatcca cggcgagatc taccgcacgc
     3361 ggcccgaggt gatggctgtg gtgcatagcc actctccgtc cgtcattccg ttcggcgtta
     3421 cgcgcaccaa gctgcgcccg atctaccaca tgggctcgtt cctgtattcg ggtgcacccg
     3481 ttttcgagat ccggaaggaa acccccgaca acgatttgct cattcgcgac aatcgtctgg
     3541 gcaaagcgct cgcgggcacg ctggggaagt gcaactgcgt gctgatgcgc ggccacggca
     3601 tgaccgtgat cggggacagc gtccccgagg ccgtgttccg cgcgatctac accgagatga
     3661 acgcgcgcct gcagctccag gcgacgcagc tcgaaggccc gatcgagttc ctcagcgagg
     3721 aggagggcag gcgctcgatg gcgtcgaacc gcggcacgct ggagcgcccc tgggagctgt
     3781 ggaagaaaag cgtcagcgcg cgaggtcgac cttgaccagc ttgtcgtgag gcagcaggcc
     3841 gaggttgagc tccagaccct gcacccggtc ggcgtaggcg gcgacgcgga tcgcgtgata
     3901 gaggaacacc atgggcgcat cctccacgat catgcgctgc gcctgcgaca gcatccggtt
     3961 ctgcgccggc ccctcgggca tgcgcagcgc ctcgtcgagg atcttgtcga gcccggggct
     4021 gcggtaatgc atgaggttcg tggtccccgc cgatctggag tggaacagcg gaagcaggaa
     4081 gcgctcggga tagggcgcgc gcacgttcca gctgtagaga aacatgtccc cttcgcgctt
     4141 gcgcacggcg gcgacgacct ccggccagcc gggcttgccg agcagctcga cctggatgcc
     4201 gatgcggcgc aggtcgctct ggatcgctcc cgcgatctcg gcggtgaagg tcagggcgct
     4261 gttgtggacc accctgaccg tcggaagcgc cgttgggccg ctcttgttca gcagttcctt
     4321 ggccgcctgc gggtcatacg gtccctggcc cacctccggg tcgtagcctc tcacccccgg
     4381 aggtagcgag cccttcgctg gcacggctac gccgcgcccc aggaacaggg cggcccgagg
     4441 cgcattgatc gcctgggcaa cggcccgccg cacgatcgcg ttgttgaacg gcggccgctc
     4501 cgtgtagaac ccgaggtagt agagattcag tcccgggaag ggcaccgact tgatgcgcgg
     4561 gctctcgcgc aggcgctcga accacacggg atcgggcagc cgtgccacga aatccacttc
     4621 gcccttgagc aaggcatcca ccaccgcttt accttccgcg atgaaacgga agaccacgcg
     4681 gtcgatctgc gggcggcctg cccagtactg gtcgttggca accagcacga tctgctcctt
     4741 cgtccagctc tcgaacttga acgggccgct gcagacggga ttgcggccga aatccttgcc
     4801 cagcttgcgc aacgccgtcg ggctgacgat ctcgctctga ctgagatagt gaagcccggg
     4861 gtacttcagc ttgagcgtga gcgtccgggc atccaatacc tggatgtcct ggacaagctc
     4921 ggccagcacc tgcgcctgca cgttgctggg ttctgccgta tagagcgggt gcgacggacg
     4981 aaactggcgc tcgatgcttg ctttgaccgc ggcggcgtcg aggggcgtcc catcgtggaa
     5041 caggacgccc gggcgcagct tcatcaccgc gctcaggccg tcggccgaaa atttccacga
     5101 ctcggcaaga cccggctcca ggctctggcc atccttcgag acgttgacca gcgcatcgca
     5161 cagcagccac gccacccgga tggcgcgctc ggtcgaagtc ttggcgaaat cgagcgactc
     5221 gggcgcgcct tccagcgcgt aggtgaacac cttgtccgcc gccgtagccg cagaggcggc
     5281 agcaaccaga atcagcgccg caaggcgcgc aagcagtctt cgggcagcta ggttcctgga
     5341 gatctcagca cccgccatta ccgcagtacc actgccagtc gcggaaccac agccgctcgg
     5401 gcatgcaatc ctgcttaaac tttttccgga gcgctggatt tacatctttc tccaaatgct
     5461 tcgtcaactc cttccaccag cggtactcgc gatagtggta gaaccactcg acatgacgga
     5521 gcatttcggc gcgattctgg tatagcgaat tcgggaaggt gtgatgaatc tcagcccgga
     5581 agctgatcat ggtctgatct gggtgcggat aatcgacctt caggtcccat tggcagggac
     5641 tgtagttcat gagaacgaag ccctccggcc atccgccggt gtctctctcg ccaggtacgc
     5701 cttctccacc ccggggagtt ctgaagactt ggccgagcac tgagagaacg tccttctcct
     5761 tgaggttgcc ctccaacctt cctccgatcc tgagcagata ctgtccgcgc gatgccatgg
     5821 ccttgacctc cccttgaatg tttgctcttt atcctcgcgt acgtgcgggc ctgcgcttgt
     5881 ccgagtgcgc cacaggtaat acaggccccc tgggttgtca agacaacgaa gctacagagg
     5941 tgctgcccag ccctcacgca gtgcccggcg agcgttcgcg tagactcccc ccgcatggcc
     6001 gatctgacct acctttgagg ccagcttggg ttcattgctg caccgctacc ggaacctgag
     6061 ccttcgcgcc aagttcgcgc tacccatcgc gctgttcgtc gtattgctgt tcggtgcgct
     6121 tattcccggg gtgctgtatc tccagacccg gaccgtcctc gacggcgccc gcgagcgcgg
     6181 gctcgagctc accaagatct tcgcgcattc gagcgtccag gccgtggtgg cggacgattt
     6241 cctggtgctg cgccagatga tcaacagcat tgcgaccgag caggacgtcc tttacgcgat
     6301 gattctggat ccgaatggac ggctgctggt ccacagcgac atgcggcagg cgggtcggac
     6361 gtacaccgat gcgcccagcc ggcgcgccat ggaaaccacg gagccgctgg tgcaggaagc
     6421 ccaggcgggc acggtgcgga tgtacgactt cgccgtgccg atctatgtcc tcaacgagcg
     6481 ccgcgccgtc gccagggtcg ggatttccct cgagcgcgag tgggcgggca tccggcgcac
     6541 gcgcaatctg gttcttgggc ttggcgtgct cgcgctcgcc atgggtctcg ggctggcaag
     6601 ctggctcgcg cggagcgtga cgcggccggt gcgagagctg gccgaaggcg cgcaggaaat
     6661 cgccgccggc aacctcgacc gcgtgatcgc ggtgcgccag atggacgaag tcgggcggct
     6721 gggggagacg ttcaaccgga tggccggctc gctgaaagcg cgctgggaga tcgaccggga
     6781 cctttcttcc acgctcgacc tggaatcggt gctcgagacg atcacgcgcc atgcgcgggg
     6841 cttgctcggc gccgacatcg cttgcgtggc tacggtcaat ccagccacca aaggcgcgac
     6901 catcgtcgcc agcgccggag cccagaccga cgcattgcgc aatctgaggg tcgctgccgg
     6961 gcgcgggctc ggcggcgaag ttctgtcgac gggacgcgcg gtgtccgtgg cggactgcgc
     7021 caaggacccg cgcatcgcgg acgagcacgg cgatctgctc aggcgcgagg ggatcgtctc
     7081 ggcgctcgtg gtgccgattt cgctcaaggg ccgcacgacc gggctgcttt acgtggccaa
     7141 ccggcgcgcg accgcgttca gccgtcagga cgaggacacg ctcaaccatc tcgcggcgca
     7201 ggcggcgatc gcgatcgaga acgcgaggct ctacgcggaa gtcacgaggc acgccgagga
     7261 tctcgagggc aaggtcgagg agcgcacgcg cgagctccag gaaaccaacc gccgcctgga
     7321 gacggcgtcg cggcacaagt ccgagttcct cgcgaacatg tcccatgagc tgcgcacgcc
     7381 gctgaacgcc atcatcggct tcacgcgcct ggtgatgcgg cgctccaagg aggtgctgcc
     7441 ggtcaagcag tacgagaacc tggagaagat tcatctcagc gccgatcagc tcctgctgct
     7501 gatcaacgac gtgctggatc tctccaagat cgaggctggc aggatggacg tgcgcccgat
     7561 cgccttcaac ctcgaatccc tcatcgacgt gtgcctgcgc accgtcgagc ccatggtcaa
     7621 gagcgagcag atccagctcg ccaaggacct cgagcccgat ctgcctccgc tcgtcaccga
     7681 ccaggacaag ttgaagcaga tcctcatgaa cctcctcagc aacgccatca agttcaccga
     7741 ggccggcacg atcacggtga gcgcccggcg ccacggcgac cgggtggcgg tggccgtcac
     7801 cgatcccggc atcggcatcc cggaggaggc gctcgatctg atcttcgagg agtttcgcca
     7861 ggtcgacagc agcagcacgc gacggcacgg cggaaccggg ctcgggcttt ccatcagccg
     7921 ccacttcgcg cgcttactgg gcggcaacgt caccgtggcg agcaggctcg ggcgaggctc
     7981 gaccttcacg ctgacgattc caatgcgata cgcactcgat gaacccgcgc cgcagccagg
     8041 ggccgcggcg ctcaagcccg gggagcccac gcacaacggc aaggtgatgc tggcgatcga
     8101 cgacgacccc gatacgatct acctgctgcg cgagaacctg atcgaggccg gctaccatgt
     8161 ggtaggcgcc ctgagcggcg aagaggggct ggagaaagca aggacgctgc gtcctttcgc
     8221 catcacgctc gatctcctga tggcgcaccg caacggctgg gacgtgcttc gtgacctgaa
     8281 gtcggacccg atcacgatgg gcatcccggt gatcgtcatc tcggtcgtgg acagcaagga
     8341 gcccgcgtac cggctcggcg ctttcgacta cctggtcaag cccttcgatc gcgacgcgat
     8401 cctgcgggcg ctggagcgca ttccgccgcc gggccggggc cgcgcggcgg tcggctgaga
     8461 tgaagaaggt gctggtgatc gaggacgtcg agctcaaccg ggagctcgtc gctcagttgc
     8521 tcgaggagga ctaccaggtg ctggttgcgg gcgatggcga gtcgggcata cggctcgcgg
     8581 cgcgcgagcg gccggacttg atcctcatgg atctctcgct ccccatcatg gacggatggg
     8641 aggcggcgcg acggatcaag gcgcacccgg acctgaagca cattccgatc atcgccgtgt
     8701 cggcgcacgc gatgcgcggc gacgccgaaa aggcgcgaaa aagcggctgc gacgattacc
     8761 tcagcaagcc gctcgacgag aatttgttgc gcgacaagct ccagaagtac ctcgggccgc
     8821 tctatcggcg ctctggcgcg cgacgcgagg agggcacgcc ccgcatcctc atcgtcgacg
     8881 acgagccgtt gaacgtcgac tacctccagc aggagctcga ggagctcggc tacgacaccc
     8941 tgagcgcccg cgacggccag gaagctctcg aatgcgtcgc caccgatccg ccggacctca
     9001 tcctgctcga catcctgatg ccggtcatgg acgggttcgc ggtctgccgc gcgctgaagg
     9061 gggacgaggc gacccggctg atcccgatcg tgatcatgac caccctcgac ggcatcgatg
     9121 accgcatccg gggcatcgag gcgggggccg acgacttcct caccaagccg gtcaatcagc
     9181 gccagctcat cgcgcggctg cagacgacac tgcggctgaa gcacacggtg gatcgcaagc
     9241 tgcgcgagct gcgccagatc aaggatcatt tcgccaagtt cgttcccgag gcggtcaagc
     9301 ggctggttgc cgagaatccc gaggcgcccg agctcgacaa gcgcgagcgg gacgtctcgg
     9361 tgctcttcgt cgacatcagc ggctacacgc ggttgagcga gcgcctgaac gcgcccacgc
     9421 tgaacgccgt ggtcgagcgc tatttctcca gcttcctcga ccgcatccac gagggtggcg
     9481 gcgacatcaa cgagaccgca ggcgacggtt tcatggcgat cttccaggat cccgacatcg
     9541 atacccacgc catcaaggcg gtggagacgg cgcttgccgt gcaggcggat accgaggcgc
     9601 tgaaccggga gagcaacgat ccgccgctcg cgatccacat cggcgtcaac tcgggggcga
     9661 ccctggtcgg ctcaacgcgc ttcgagggtc tgcggggggc ccgttggacc ttcaccgcca
     9721 ccggcaacgt caccaacctc gcggctcgcc tcgccgcggc tgccgagtcg ggccagatca
     9781 tcgcgggacc cgagaccgtg cgccggctgg gaagccgcta tcagctcgaa cggctggcgg
     9841 tggagcggct caagaacatc gccgccgaca tcccgctgca tcgcgtgctc gggccgagcg
     9901 cggcgcgctg actagccggc cgccgccacg gcccgaccgg ggagagaccg cctcagcagc
     9961 cagaaggcga tcgtcaggtt gagcaggttg aacccgatgc cgttgatgaa cgcggcgcga
    10021 taggagccgg tcatgtcgaa gatcgccccc gacacccagc cgccgaacgc catgccgaag
    10081 agcgtcgcca tcagcacgat tcccgtgcgc acgccggctt cgcgcggcgg gaagtattcg
    10141 cgcacgatga tcgcgtagga cggaacgatg ccgccctgga acaggccgaa catcgccgag
    10201 acgacgtaga gcgagacaag gccgtcgaac ggcaggaaca tgacgagcgc gatgccctgg
    10261 agcatggagc cgaggagcag ggtgcgcagc ccgccgatgc ggtcgcagat ccagcccgag
    10321 acgaggcggc tgacgatgcc gaaggcgagc atgatcgaga gcatctgcgc gccctgcgcg
    10381 gcgccgtagc cgaggtcgcc gcagtacgcg acgatgtgaa cctgcggcat cgacatcgcc
    10441 acgcagcagc ccacgccggc gatgacgagc agcaccatca gcgcattcgg cgacaggccg
    10501 agaggacgtt ccccgaaaga ccttgcctgg atcgcttgac tagagatgtc caatgccggc
    10561 ggccgccggc gcatggccaa tgcaagcagc aacatcgcgg ccgcgcagaa gatcccgagg
    10621 cctacgtacg tcgaacgcca gcctaccgtg tcgaaggagt actggaggat cggcggccag
    10681 acggcgccgg cgaggtagtt gccgctcgcg cagatcgcga ccgcgatgcc gcgccggcgg
    10741 ttgaaccaca aggacgtgtc cgcgacgagg ggcgcgaacg tggtcgagca tccgaggaat
    10801 ccgatcatca atccatgcgc caggctgaac tgcaggaggc ttcccgccgt acccgccagc
    10861 acaaagccgc tgccgaggcc gagcgcgccg atcacgaccg ggaccaccac gccgaagcgg
    10921 tcggcgatgc gccccatcag gatgccgccg atgccgaagc cgagcatcgt gagcgtgtag
    10981 gggagggaag catcgccgcg cgtgacgccg aactccgctt gcaccggcgc aagcgcgact
    11041 gcgatgctgt acatgcccga gccgccgagc gtcatcaggg cgagtgcggc gagaaggcgc
    11101 agccaggcgt aggttgattc cggttcgctt ttcaccggtt catagtatcg tcgcgctttc
    11161 catccgagga ggactgtttc atggccgcaa gacagccacc gtcgaccatc gcgcaggtgc
    11221 gcgacgtgtt caccaagctg ggcgagatca gcgacaaggt cgtgttcggc gacgtctggg
    11281 agcgcaagca gctctcgaag cgggatcgca gtttgatcac ggttgcggcg ctaactgcgc
    11341 tctaccgccc cgatcagctg cgcggccacc tgcaccgcgc gttcgacaac ggcgtcacca
    11401 aggaggaaat catcgaggtg atcacgcacc tgtcgttcta tgccggctgg ccgaacggcg
    11461 gcacggccgc gctgatcgcc aaggaagtgt tcaaggaacg cggcgtatga agatcggctt
    11521 catcggcctg ggcgccatgg gccggccgat ggccgcgagc ctgcagcgcg ccggccatgc
    11581 gctccaggtt tacgacctgc ggcgcgtcga aggtttcccg aactggaaag actccgcggc
    11641 gaaagccgcc gaggcatgcg agctcctctt cacgtcgctg ccggggccgg ctgaggtcga
    11701 agccgttgcc gcgcatctgg agtctcctgt ggtctggttc gacctgagca cgaattcgcc
    11761 gaaggttatc cgcaggcttc acgccgggtt acaaaagaaa cggatcgagc tcctcgatgc
    11821 cccggtgagc ggcgggccca aaggcgcgca gagcgggaag ctggcgatct gggtcggcgg
    11881 cgagcaggcg gtgttcgaaa agttcaagcc cgtgctgcaa gcgatcggag accaggtcct
    11941 gtacgtgggg tcgatcggcg cgggcacggt ggccaagctc gcgcacaacg ccgccagctt
    12001 caccatccag gcggcgctcg cggaggtgtt cacgctcggc gtcaaggcgg gcgtcgagcc
    12061 gttggcgctg ttgcacgcgc tgcgccaggg cgcgaccggc cgcaagcgca ccttcgaccg
    12121 gctggcggag catttcctgc cgggcgtcta cgacccgccg gctttcacgg tgcggcttgc
    12181 ccagaaggac gtggcgttgg cgctcgagct ggcgaaggaa tgcggcgtgc cgatgcgcgt
    12241 cggaaaaatc gcgctggacg agctcgacga ggcgatcaag cgaggctggg gcgagcgcga
    12301 ctgccgtgta gcgatgacgc tacaggagga gcgcgccgcg gtcagcgttc gcgtgccgcg
    12361 cgaacgtctg gagaatttgc cggactaagg atcggtatgg gtcgcggacg gctggtgatg
    12421 gccgtaatca tcgcgctgat ggcgatcatc acctatttca gcagcacgag cgagaatccg
    12481 ctcacgggcg agaagcagcg cgtcgccatg acgcccgaac aggagatcgc gctcggctac
    12541 aagagcgcgc cgcagatggc cgcgcagatg ggcggcctgt cgcagaacga gaaggcaggc
    12601 gcgctggtga agcaggtggg cgagaagctg gtcgccgaga gcgtcgccgc gaaaagcccc
    12661 tacaagttca gcttccacgt gctcgccgac ccgcgcacgg tcaacgcctt cgcgcttccc
    12721 ggcggcccga tcttcatcac cgagggcctg ctgcggctcc tcaagtcgga agccgagctc
    12781 gccggcgtgc tcggccacga gatcgggcac gtgatcgcgc gccactcgtc cgagcggctc
    12841 gccaagcagc agctcaccca gggactgctc ggcgcgctgg tggtgggcag cggcgactac
    12901 agcacagcgc agatcggcca ggtggtcggc agcatgatca acatgagcta cgggcgagag
    12961 gacgagctcg agtccgatgc tctcgggatc cgaatcatgg ccgaggcgcg ctacgacccg
    13021 cgcgcgatga tccgcgtcat ggaagtcctg gcgaaggctt cgggcggctc acgtcagccg
    13081 gaattcgtct cgacgcatcc cgcgcccgag aaccgctcgg cgcgcatcaa ggcggaaatc
    13141 gagaagcggt ttcctcaggg cgtgccgaaa gggctcaagg agtaggactc gagccggaat
    13201 cccaggcgat cgcatcccgg ggtggagaaa gtcgcgaggc gaccgaacgc ctgcagcacg
    13261 cagatcgagc cgaagggccg atatccgacg cgcaagcagg ctttcagcga atcgaagttg
    13321 gtcgacgcga cgtaggcgac gaacccgcag aagccgcgcg ccaggtaatg ccgcagcgcg
    13381 agcgtcgtgc cgaaggcgtg aaggcgccgt ccccggtagg gcgccagcgt gaaccccttg
    13441 tagcggtaga cccagcgggg gtcgaaatgc aggagcgttt ccggcagatg cgtcggcgta
    13501 ggccgaaagg cgtaccaccc gtaggccaca agactggggc cgtcgcagat cgcaaagcac
    13561 tgatcccctc gcgacagggc ctgttgcacg aatttcagcg acagctcgct gccgggttgc
    13621 tcgacgaatc cgtacagcgc ctctcgcgat gcgaagctcg cggtgtatcc ggccgggcat
    13681 cccaggaacg acgggtctac ctgcgccagg cacatcgcgc gcaggatcct gaaagatacg
    13741 gctgaattga cggcgcgcat gccgtacgcg tggagcgtgg ccgtcagtcc atgccggcgc
    13801 agtctgcggc ggacctttgc gacgatcggc ggttcggcga ggctcagcat ggcgggctcg
    13861 ttgtccgaag gagcaacatt gggaccgtca cccgaccaag ggccaatcgg cgcatgcggt
    13921 gggcgggatg ctaaactgca ggctggcaac tttgaaagga ggtgatccag tgagagacca
    13981 tcaaggcagg accggcgggc ccaactccgg atcaaccagc aacggcatca ggagcgacgc
    14041 cagcaagtaa gccggctctc gccttagcaa gcgccccgac cgcgaaagcg ctcggggcgt
    14101 ttcatttcta gaacctgatc ggcaattctt tgaggccgcg aagcgtcgag gactcgcgcc
    14161 attgcggcgt tccggtggcg agctcgagct gcggcaggcg gcgaagcagc gtgccgagcg
    14221 caatctgcgc ttcgatgcgc gcgagcggcg cgccgaggca gaagtggatg ccgaagccga
    14281 acgcgatgtg gtccttgtcg ggccgggcga ggtcgaggcg gtcgggatcc gggaaacgct
    14341 ccgggtcgcg gttggccgcg ccgatcgcgg caacgaccag ggcgtgccgc ggaatcgtct
    14401 tgccgcccag ctccacctcg gtcttggtga cgcgagccgt gcgctgcacc gggctgtcgt
    14461 agcgcaggag ctcctccacc gcgctcgcta gcagttgggt attctctttc aatacttcca
    14521 gttggtccgg atgctggagg agggcgagga gcccgttgcc gatcaggttc accgtggtct
    14581 cgtggccggc gatgaagatc agcacgcagg tggcgaggag ctcgtcctcg gtgagcttgt
    14641 cgccctgctc ctcggcggcg atgaggaggc tcagtaaatc cgcccgagga ttgcgccggc
    14701 gctcgggaag cagcccgcgg aaatattcgc ccagcgcgtg tcgcgcggcg cgtccgcgcc
    14761 tgacgatctc gcggtccgac ggcagcccga gcgcgtccag gctgcgcgtg atgtccgcgg
    14821 accatcgccg catcgagtcg tggtcgcagt cgggcacgcc gagcatctcg cagatcaccg
    14881 tgaccgggag cggataggcg agcgccgaga tcacgtccat cgctccggcg ccctggacgc
    14941 ggtcgagcag ccggtcgacg atctcctgga tgtgggggcg catgccctgc accacgcggg
    15001 tggtgaaagc cttgctgacg agcgagcgca ggcgcgtgtg cgccggcggg tcgcggaaca
    15061 ccatcgggcg cggcacccgc tccgagtctt cgccgtacac cgcgctcacc atcggcccga
    15121 agtcttcgcg cccgaagcgc gcgtcgcgca gcaccgtcac gacgtcggag tagcgcgtga
    15181 cgacccagaa tcccatcgcg ctctggtgca ccgggtcctg gagtctcagc cgatggtaga
    15241 aggggtacgg gttggcgtgg aattccggca gggccgggtc gaacaggggc tgactctgaa
    15301 cggatccgga tatttcccgg gttgcggctt ccgctttctc ttcccagaac cgcatctcca
    15361 gctcgcccag cagctcgcgc gccagcgtga gctgctcgag cgcctgggcg cgcttgccgg
    15421 ccttgccgta cagcatgccg agatgcagat ggcttcgggc aagcaccggg cgcagcccga
    15481 gctccgatgc gagggtgatt gcctcgcggt aggaggactc ggccttggcg aaattcggcg
    15541 cctcgcccgc cgcgtggata tccccgagga cgcggaggat ctcgggctcg gtaaagcgct
    15601 ggcggtgaag ccgcagcagc gcgaggccgt gctcggcggc ctcgagggcc tgggcgcggc
    15661 ggccggcggc aaggcgtgcc tcggccaggt tcgtagcctc cagcgcgtcg aacggcacga
    15721 tgtgcatgcc cgacggttgc gcgctcgagc gctcgaggag ctcgaggcct tcctcgatgc
    15781 gcccgcagcg cacgtaggcg aggccgaggt tcggcgcggt gtagcggtag agcggcccga
    15841 agttgccgcg ccggcacagg tcgagggcgc gctcgaggag cgggatcgcc tgcgcgagct
    15901 cgccctgcat ctggtggcgc tgtcctgcgc cggaatacgt cgtggccagg ctgtagggat
    15961 ggttcgcttc ctcggccacg cggatgcttt cccgcgccac cgcttttgcg acgccgaact
    16021 ctccgacgct ggcgaggcac caggccatcc aggagcgcga cgtcaccgat tgcagcgcag
    16081 ccatgccgaa gcgcttcggc gagggcgcct tggtgcgcag ctcgaggttc ctctcgaagc
    16141 tcgcgatagc ttgccggtag ttgcccaggc tgacatgggc ctcgcccacg cggtgattgg
    16201 acaccgcctc gagcgcgccg tcctcgatct cctcggcgat cgccagcgcg cgccggccgc
    16261 actcgagcgc gcggtccgga tcgccgatgc accagtacca ggtcgccatc gacgccgaca
    16321 ctcttccctg gcgggcgcgg tcgcccagac ggtcggcgag cttctcggcg tcgcgcagga
    16381 cttccacgat gcgcgaaaac tcgccgagcg gaatcagggc gctatgcagg tcgaagcgca
    16441 ggtcgatgcc ctgcgcgatg gtttcggcgc tctcggggag atgtctcaag gcgaagagcg
    16501 cctgctcgac gcaggccacc gcttcgccgg aggcgctgcg cgcggtcgcc ttgaggccct
    16561 cctcgtgcag gtaggcgatc gccttggtcc acagctcgcc ccgcagcgcg tggtaggcga
    16621 gccggtcgat ctggtcgctc aatcccggcc cgggcatgga ttccagcgcc tccacgatcc
    16681 gtccgtgcag ctcccggcgg gtctcgtgca gcagggcgtt gtaggcgacc tcctgcgtaa
    16741 gcgcgtgctt gaacgcgtac tcgagctcgg gaaagaggct cgtctcgtaa aggaactcgg
    16801 ccgcctggag gcgggcaagc agcccgcgca gggtgtcctc atcgtgctcg ctgatcgcct
    16861 gcagcaggga gaagggcacg ctgcggccga tcacggcggc ggactgcagc agccatttct
    16921 cgtcggccga aaggcggtcg atccgcgcgg cgaggacggc ctgcacggtc ggcggcacct
    16981 gggcggtttt caggtccttg accaggcggt acgactcccg gtcgcgcgcg atgacgccgg
    17041 tctcgatcaa cgcccgcacg ccctcctcga ggaagaacgg attgccgccg gtgcgctgga
    17101 tgagcatgtc ggccacgggc ttgagcgtcg gctggtcgcc gatcagcgtc ttcagcagct
    17161 cgccggcgcc ggcggcgctg agcgggtcga tgcgcaactg cgagtggtag cccctgtctc
    17221 cccatttgtg gcggtattcc tggcggtagg tcgcgagggc gagcaccggc agcgcgggaa
    17281 tgctctccac catgccgtcg agcagcgcct cgctttcggc atcgaaggcg tgcacgtcct
    17341 cgaacaccac gaggagcggt tgcgcctcgc tctggcgcag caggaggcgc cgcagcgcgt
    17401 cgaacgtttg ccagcggcgc tgcgacggat tgagctcgtc ccactccgcg tcggcgacag
    17461 gaacgtccag cagcgcgagg agcgcgggca gaatcggctc gagcgtgcgg tcgaggctgc
    17521 gcaccgcttc gccgaccttg cggcggatct gcgcgtggtc gtcgcggctg tcgatcttga
    17581 agtactcgcg cagcagcagg atcaccggaa ggtaggacgt ggcgggaccg taggaattgg
    17641 gcccggtctt gaggaccagc cagtcccgcg catcggcaga gctcaggaac tcgtgcacga
    17701 ggcgcgactt gcctacgccg ggctctccca cgatggacac gacctgtccc tggcccgcgc
    17761 gggcgagccc gattgccgag cgcaggcgcg ccatcgcggc ctcgcgtcct gtaaagcgcg
    17821 tgaggccgcg cgccagcgcc gcctgcaggc gcgtgcggac cggtccggcg ccagtgactt
    17881 cgtagacttc caccggcgac gagagtccct tgacctgcac gggtccgatc ggccgcgcct
    17941 ccacgtagcc ttcggcggcg aggaggccgt cggcggtgat caggatctgc ccgggctccg
    18001 cgatctgctc catgcgcgcc gcgaggtgcg tggtctggcc gaccgcgctg tagtccatgt
    18061 gcaggtcgct gccgaccgag cgcaccacca cctcgccgga gttcaggccg acgcgcacgc
    18121 gcacgtcgac c
//
LOCUS       DATBSZ010000198        10461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1537121, whole genome shotgun sequence.
ACCESSION   DATBSZ010000198 DATBSZ010000000
VERSION     DATBSZ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10461
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537121"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..825)
                     /locus_tag="VIG70_12610"
     CDS             complement(28..825)
                     /locus_tag="VIG70_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY8251439.1"
                     /translation="MARLHDDEIAHYRTEGWVVPRFRLPPARMASMVDALEDLLRRNP
                     GVRPEKLVSAHIEGDNGEGVRGSRAFLDLARDPEIVELVSGVIGEDVILWGCHVFCKP
                     AVEGYETPWHQDGHYWPIRPLANCTAWVALEPSTVENGCLRVIPRSHAERRLHEHLHE
                     DRTDLTLNQRMADGVFDEAAAVDIELEPGQMSLHDVYMIHGARANTSGKRRTGAALRY
                     MPSTSVFERSLRPAHGQTGVPVNFARRPLWLLRGVDRSGRNDFSVGH"
     gene            complement(831..1994)
                     /locus_tag="VIG70_12615"
     CDS             complement(831..1994)
                     /locus_tag="VIG70_12615"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEY8251440.1"
                     /translation="MKRSARRILTTHVGSLPRPADLLRAVLAKDRGEAFDEKAFPGLL
                     ERSVRQIVRKQAEIGLDVIDDGEFGKPGFMHYVNQRLSGFEPIRGAAPASPFAASRDY
                     RSFPEYYAAQPASPAQKAPHLECTGPISYKGQRLLAADIANLKAAAAEAKVEEAFMPA
                     VSPSNVEEWQRNRYYRTDEEFVFAIAEAMREEYKAIVDAGLLLQIDDPRLVTRYNVTP
                     EASVADCRAWAAVRVEALNHALRGLPRNRIRFHTCYGINIGPRVHDMELKDVVDLILK
                     VNAGAYSFEAANPRHEHEWTVWKDVKLPDGALLIPGVITQSGVVVEHPELVAQRLERF
                     AGAVGRDNVIAGADCGFGTFAGSEEIQERIVWAKLEALVQGARIASERLWRRG"
     gene            complement(2005..2988)
                     /locus_tag="VIG70_12620"
     CDS             complement(2005..2988)
                     /locus_tag="VIG70_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEY8251441.1"
                     /translation="MNANLQAKPVILGSGEHRYRVLESWAKLPEGWELKDVGAVAVDS
                     KDRVYVFNRGEHPMIVFDREGNFIRSWGEGTFPRAHGLHIDANDILYCTDDGGHLVRK
                     CTTEGRVLLELGVPGQAAPYMSGTPFHRCTHTALSPRGDIYVSDGYGNARVHKFSPDG
                     KLLKSWGEPGTDPGQFNIVHNIVTDAEGWVYVADRENHRVQVFDGNGRYETQWVNLHR
                     PCALYCCGGARPQFVIGELGPGMTVNRSHPNLGPRLSFVDGKGKLVARLGGEQGPGLE
                     TGKFIAPHGLAVDSRGDLYVGEVSYTEFPRLFPEQKIAWRMRSLQKLEKLA"
     gene            complement(2985..4214)
                     /locus_tag="VIG70_12625"
     CDS             complement(2985..4214)
                     /locus_tag="VIG70_12625"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8251442.1"
                     /translation="MKRDKPSALDTVETCLAAIEARDGELHAWKAVDPEFARRQARAL
                     DASPVHGPLQGIPVGVKDIFDTADLPTEYGSPIYKGHRPAADAACVALARAAGAVIVG
                     KTVTTEFATMVPGATVHPHDPRRTPGGSSSGSAAAVAAGMVRFAYGTQTVGSTIRPAA
                     YCGVVGYKPSYGMISRSGMKMGAESLDTVGIIARNVADAALLAAASSMRDDLLEIERV
                     EKPRLGVCRSPNWHHMSAEGIESFESAVERLAKKGARIVEKEMPLDELDAASAIILVY
                     EVARGLAHEVVNHRSSISPMLLERLDAGEAMQYPQYAKAIAYAASCRWRARDACSDID
                     AVLTPSATGEAPLGLHSTGNTAMNRLWTLLHGPCVTVPAGVGPAGMPLGIQLVGLPGD
                     DAWTLAVAAWAEHVLAS"
     gene            4232..5554
                     /locus_tag="VIG70_12630"
     CDS             4232..5554
                     /locus_tag="VIG70_12630"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8251443.1"
                     /translation="MAPHELERLATLVRPDRVHRSVYADPAIFDLEMERVFGRAWLVL
                     GHESQVRAPGDYFTTRMGREPVIVIKKEDQTVGVLINRCAHRGSMVCAEGSGRLSTPG
                     ERFVCPYHGWSYDRAGTLQAVPFAGGYDKGKLPAGLKAVPRVKTYRGFIFASLSAQGE
                     DLETFLGPARASFDDFVDRAPGGELEVAGGVFKHAYNGNWKLMLENHLDGAHPAWVHA
                     SSVAVARAAPEPGRPGEEHYYDIAVRQMRQNGAPDAVWEAIGMWTTPHGHGYMGDYHD
                     DSRLVTGLGNPVFDEYRKRLIKHVGDKEADRILRVTLWNTIIYPNCSFMSQFRQLRII
                     HPIAVDRSVVYTYSFRMKDAPAQMFRDTVAFANVVNGTGSWVLTDDLEVYERLQRGLS
                     SGEVDWVYIGRGHGRDVEEPNGVRRGATGTSEIFMRGQFAAWLRYLSQ"
     gene            5551..6009
                     /locus_tag="VIG70_12635"
     CDS             5551..6009
                     /locus_tag="VIG70_12635"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEY8251444.1"
                     /translation="MNVEQFLYHEARLLDTGQLEAWLELFTDDATYWLPLEHDQKDPL
                     ETSSIIHDDRTLLGLRVKQARHPRAHARLPLARTVHQVGNIMVLSEKNSEIQIASTLQ
                     LVEFRNEKQRLYGALVEHRLRPVNGSFKIAHKRVDLVNSEGELDGIAILF"
     gene            6006..6794
                     /locus_tag="VIG70_12640"
     CDS             6006..6794
                     /locus_tag="VIG70_12640"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8251445.1"
                     /translation="MTKVFRDYDQKALDDQYEQRIWVPHADEIIKRYARKSDEVRSRL
                     GEPRVEKYGSTAVETLDIYGSGQKAFVFVHGGAWKRQSSREQAFPAEAIIEAGAAYVA
                     LNFANLPTVTLPEMAAQVRRGIEWVRKNLSDYVVLCGHSSGGHLSACALTRLDLVKKA
                     LVVSGVYDLLPVRLSARNEYVRLDERLEHEYSPIRHVDRIRCPVTVAWGEKEGAEFFR
                     QSKEFAEKLGAPTILGKGLNHFEIIETLGDPRSALGRAALNMLQ"
     gene            6791..8143
                     /locus_tag="VIG70_12645"
     CDS             6791..8143
                     /locus_tag="VIG70_12645"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HEY8251446.1"
                     /translation="MKARNEKPVVIAGGGIGGLAAALALARKGFRSVVLEQAPQFGEI
                     GAGIQIAPNAWHALDALGVGALVKKEAVFVEHLFMFDGVSGEKVIDIPLDKRFAKRFG
                     NPYAVTHRADIHGSLLGGCTALPEMIELRTSTRVTAFEQDGASVRVKTDNGSIEGTAL
                     VGADGGRSVIREKIVADAAMPVSGHMCYRAVLNIGDVPKALRLPAATLWAGHNTHIVH
                     YPLRGWKLFNLVATVIGKHSSGGHNELALPEEVLPLFTHYCEKPTKLMRTPREFRRWM
                     LAYREPVDNWTRGRVTLLGDAAHFMLQYMAQGAAMAMEDAVCLGACADEADGQFEKAF
                     SNYQDRRIVRATRVQVSANQLVGMIFHVPDGLEREVRNDIYRGRSAERYYDALEWIFS
                     APEYVRNFSKKAGPARSPRPIVSRRAASRAPARSTGSRARGARPARRPPSRRRASPAR
                     "
     gene            complement(8058..9134)
                     /locus_tag="VIG70_12650"
                     /pseudo
     CDS             complement(8058..9134)
                     /locus_tag="VIG70_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
     gene            complement(9118..9387)
                     /locus_tag="VIG70_12655"
     CDS             complement(9118..9387)
                     /locus_tag="VIG70_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251447.1"
                     /translation="MPPVRQILCILLLALPPAAHAGGVVVVGSSGTSGFWLALAVLAG
                     FVPPPPAPEVSPHHLSAGPAPAKDSCTFVAGVRHCPLAKPRAESR"
     gene            9416..10351
                     /locus_tag="VIG70_12660"
     CDS             9416..10351
                     /locus_tag="VIG70_12660"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY8251448.1"
                     /translation="MKPGNKVKRIEGVLSPVITPFKKDYSPDAERFVRHCRWLLKSGC
                     AGLAVFGTNSEANSMSVSEKRHLLEALVKGGVPASALMPGTGHCALSDSVKMTREAVR
                     LGCGGVLMLPPFYYKGVSDEGLYRNFAEVIERVGDERLSLYLYHIPPVAQVAITLNLI
                     EKLLSKYPGIVAGVKDSSGEWSNTKAMLDNYAKGGFDVFAGSEVFLLDNMRNGGKGCI
                     TATGNVNPGPIANVYKNWRSTEADKLQAGITATRKIVQKQPMIPALKATVAHFGNDPQ
                     WKTCRPPLTELTAAQEEELIRDLKGAGFSMPGLAA"
BASE COUNT         1743 a         3507 c         3538 g         1673 t
ORIGIN      
        1 ggccgtctcg acgccctcga tcctcactca gtgaccgacg gagaaatcgt tgcgtccgct
       61 gcggtccacg cctcgtagca gccacagtgg gcgccgcgcg aagttcaccg gcacgccggt
      121 ctgtccgtgg gcgggtcgga ggctccgttc gaacaccgac gtcgaaggca tgtagcgcag
      181 cgcggccccg gtgcggcgct tgccggaagt gttggcgcgt gcgccatgga tcatgtagac
      241 gtcgtgcagc gacatctggc cgggctcgag ctcgatgtcc accgccgcgg cctcgtcgaa
      301 cacgccatcg gccatgcgct ggttgagcgt gaggtcggtg cggtcctcgt gcagatgctc
      361 gtgcaggcgc cgctcggcgt gcgagcgcgg gatcacacgc aggcagccgt tctccaccgt
      421 cgagggctcg agggcgaccc aggcggtgca gttcgcgagc gggcggatcg gccagtagtg
      481 gccatcctgg tgccagggcg tctcgtagcc ctccaccgcc ggcttgcaga acacatgaca
      541 gccccagagg atcacgtcct cgccgataac cccggagacg agctctacga tctcagggtc
      601 gcgcgcgagg tcgaggaacg cgcggctgcc gcgcacgcct tcgccgttgt cgccttcgat
      661 gtgcgccgag accagcttct cgggccgcac ccccgggttc ctgcgcagca ggtcctccag
      721 cgcatcgacc atgctcgcca tccgcgccgg cggcagccgg aagcgcggga ccacccagcc
      781 ttcggtccgg tagtgcgcga tctcatcgtc gtgcaatcga gccatatttg tcagccgcgc
      841 cgccagagcc gctcgctcgc gatgcgtgcg ccctgcacca gcgcctcgag cttcgcccac
      901 acgatgcgct cctggatctc ctccgagccg gcgaaggtgc cgaagccgca atcggcgccg
      961 gcgatcacgt tgtcgcgccc gaccgcgccg gcgaagcgct ccaggcgctg cgccaccagc
     1021 tcgggatgct cgaccaccac gcccgactgc gtgatcaccc ccgggatgag cagcgcgccg
     1081 tcggggagct tcacgtcctt ccataccgtc cactcgtgct cgtggcgcgg gttggccgcc
     1141 tcgaacgagt atgcgccggc gttcaccttg aggatcaggt cgacgacgtc tttcagctcc
     1201 atgtcgtgca cccgcggccc gatgttgatg ccgtagcagg tatggaagcg gatgcggttg
     1261 cgcggcaggc cgcgcaacgc gtgattcagc gcctcgacgc gcaccgcggc ccaggcgcgg
     1321 cagtcggcga cgctcgcttc cggcgtcacg ttgtagcgcg tgacgagcct gggatcgtcg
     1381 atctgcagga gcagccccgc gtcgacaatc gccttgtatt cctcgcgcat cgcctcggcg
     1441 atcgcgaaca cgaattcctc gtcggtccgg tagtagcggt tgcgctgcca ctcctcgaca
     1501 ttggagggcg aaaccgccgg catgaaggct tcttccacct tcgcttccgc agccgccgct
     1561 ttcaggttcg cgatgtcggc cgccagcagc cgctggcctt tgtacgagat cggacccgtg
     1621 cattccaggt gcggcgcctt ctgcgccggc gaggcgggct gcgccgcgta gtactcgggg
     1681 aacgaacggt agtcgcgcga ggctgcgaac gggctcgccg gcgccgcgcc gcgtatcggc
     1741 tcgaagccgg acaggcgctg gttgacgtag tgcatgaacc ccggcttgcc gaactcgccg
     1801 tcatcgatga cgtcgaggcc gatctcggcc tgcttgcgca cgatctggcg caccgatcgc
     1861 tcgagcaaac ccgggaaagc cttttcgtcg aaagcttccc cccgatcctt cgccagcacc
     1921 gctcgcagca gatcggccgg ccgcggcagg ctgccgacgt gggtggtgag gatgcgccgg
     1981 gcgctgcgct tcatcccggc gcgctcaggc gagcttttcc agcttctgca gcgagcgcat
     2041 gcgccaggcg atcttctgct ccgggaagag gcgcgggaac tcggtgtagc tcacctcgcc
     2101 gacgtagagg tcgccgcgcg agtcgacggc gaggccgtgc ggcgcgatga acttgcccgt
     2161 ctcgaggccc ggcccctgct cgccgcccag gcgcgcgacg agcttcccct tgccgtcgac
     2221 gaacgacagg cgcggcccga ggttggggtg gctgcggttg accgtcatcc cggggccgag
     2281 ctcgccgatc acgaactgcg gccgcgcgcc gccgcagcag tagagggcgc acgggcgatg
     2341 caggttcacc cactgcgtct cgtaccggcc gttcccgtcg aacacctgca cgcggtggtt
     2401 ctcgcggtcg gccacgtaca cccagccctc ggcgtcggtc acgatgttgt gcacgatatt
     2461 gaactgcccg ggatcggtgc ccggctcgcc ccaggacttg aggagcttgc cgtcgggcga
     2521 gaacttgtgc acgcgggcgt tgccatagcc gtcgctcacg tagatgtcgc ctcgcggcga
     2581 gagcgcggtg tgcgtgcagc gatggaaagg cgtgccggac atgtagggcg cagcctgccc
     2641 cggaacgccg agctccagca gaactcttcc ctcggtcgtg cacttgcgca cgagatgccc
     2701 gccgtcgtcg gtgcagtaga ggatgtcgtt cgcgtcgatg tgcagcccgt gcgcgcgcgg
     2761 aaacgtgccc tcgccccacg agcggatgaa gttgccctcg cggtcgaaca cgatcatcgg
     2821 gtgctcgccg cggttgaaga cgtagacgcg gtccttgctg tcgaccgcca ccgcgccgac
     2881 gtccttcagc tcccagcctt cgggcagctt cgcccagctc tcgagcaccc ggtagcggtg
     2941 ctcgccgctg ccgaggatga ccggctttgc ttgaagattg gcattcatga cgcgagaacg
     3001 tgctccgccc acgcggcgac ggcgagcgtc caggcatcgt ccccgggcag gccgacgagc
     3061 tggataccga gcggcatgcc ggcaggaccc acgccggcgg gaacggtcac gcagggcccg
     3121 tgcagcaggg tccacaagcg attcatcgcg gtgttgcccg tgctgtgaag accgagcggc
     3181 gcctctcccg tggcgctcgg ggtcaggacg gcatcgatat cggagcaggc gtccctcgcc
     3241 cgccaccggc aactcgccgc ataggcgatc gcctttgcat actgcgggta ctgcatggct
     3301 tcgccggcat cgaggcgctc caggagcatc gggctgatgc tcgagcggtg attgacgact
     3361 tcgtgcgcca ggccgcgcgc cacctcgtag acgaggatga tcgccgaggc cgcgtccagc
     3421 tcgtcgagcg gcatttcctt ttcgacgatg cgggcgccct tcttggcaag gcgctcgact
     3481 gcgctttcga acgactcgat gccctcggcg gacatgtggt gccagttggg cgagcggcag
     3541 acgccgaggc gcggcttctc cactcgctcg atttcgagca ggtcgtcgcg catggagctt
     3601 gccgcagcca ggagcgctgc gtccgcgaca ttgcgcgcga tgatccccac ggtgtcgagc
     3661 gactcggcgc ccatcttcat gcccgagcgg ctgatcatgc cgtagctcgg cttgtagccc
     3721 accacgccgc agtaggcggc cgggcgaatc gtcgagccga ccgtctgcgt gccatacgcg
     3781 aagcgcacca tcccggccgc caccgcggcg gcgctgccgc tggaggagcc gccgggcgtg
     3841 cggcgcgggt cgtgcggatg caccgtggcg cccggcacca tggtggcgaa ctcggtggtg
     3901 acggtcttgc cgacgatcac cgcgccggcg gcgcgcgcca gcgcgacgca agccgcatcc
     3961 gccgcggggc ggtgcccctt gtagatcggg ctgccgtact cggtcggaag gtccgcggtg
     4021 tcgaaaatgt ccttcacccc gaccgggatg ccttggagag ggccgtgcac cgggctcgcg
     4081 tcgagcgcgc gcgcctgccg gcgcgcaaac tcggggtcga ccgccttcca ggcgtggagc
     4141 tcgccatcgc gcgcttcgat cgccgcgagg caggtttcga ccgtgtcgag cgcgctcggc
     4201 ttgtcgcgtt tcattagaat caatgatagc catggcgcct cacgaactgg agcgattagc
     4261 gacgctggtt cgacccgacc gcgtgcaccg ctcggtgtac gccgacccgg cgattttcga
     4321 cctggagatg gagcgcgtct tcggccgcgc ctggctggtg cttgggcacg agagccaggt
     4381 aagagcgccc ggcgactact ttacgacccg catggggcgg gagcctgtaa ttgtcatcaa
     4441 aaaagaagat cagaccgtcg gcgtgctgat caaccgctgc gcgcaccggg gctcgatggt
     4501 gtgcgccgaa ggaagcggtc gcctttccac cccaggggag cgtttcgtat gcccctatca
     4561 cggttggagc tacgaccgcg ccgggaccct gcaagcggtg ccgttcgccg gcggatatga
     4621 caaggggaag ctgccagcgg gcctgaaggc cgttcccagg gtgaaaacct atcgaggctt
     4681 tatctttgcg tcgctttctg cacaagggga ggacctggaa acgttcctcg gccccgcccg
     4741 ggcttcgttc gacgacttcg tcgaccgcgc gcccggcggc gagctcgaag tggcaggcgg
     4801 cgtgttcaag cacgcctaca acggcaactg gaagctgatg ctcgagaacc acctcgacgg
     4861 cgcgcatccg gcttgggtgc acgcctcctc ggtggcggtg gcgcgcgcgg cgcccgagcc
     4921 gggcaggccg ggcgaggagc actactacga catcgcggtg cgccagatgc gccagaacgg
     4981 tgcgccggat gcggtatggg aagcgatcgg catgtggacc acgccgcacg gccacggcta
     5041 catgggcgac taccacgacg acagccggct ggtcaccggc ctgggcaacc cggtgttcga
     5101 tgaataccgg aaacgactca tcaagcacgt gggagacaag gaagcggatc gcatcctgcg
     5161 cgtcacgctc tggaacacca tcatctaccc gaactgctcg ttcatgagcc agttccggca
     5221 actgcgcatc attcacccga tcgccgtcga tcgcagcgtg gtttacacct actcgttccg
     5281 aatgaaagac gcgccggcgc agatgttccg cgacacggtg gcgttcgcca acgtagtgaa
     5341 cggcaccggc tcctgggtcc tgaccgacga cctggaggtc tacgagcgcc tgcagcgcgg
     5401 cctctcctcg ggagaggtcg actgggttta catcggccgc ggccatggcc gcgacgtgga
     5461 ggagccgaac ggcgtgcggc gcggcgccac cggcacgagc gagatcttca tgcgcgggca
     5521 gttcgctgcc tggctgcgct atttgtcgca gtgaacgtcg agcaatttct gtatcacgag
     5581 gcaaggctgc tcgatacggg tcagctcgaa gcatggctcg agctcttcac cgacgacgcc
     5641 acgtactggc ttcccctgga gcatgatcag aaggatccgc tcgagaccag ctcgatcatc
     5701 cacgacgacc gcacgctcct cgggctgcgc gtcaagcagg cgcgtcatcc gcgcgcccat
     5761 gcccggctgc cgctggcgag gacggtgcat caggtcggga atatcatggt tctttcagag
     5821 aaaaattctg aaatccaaat cgcttcaacg ctccagctcg tcgagttccg caacgagaag
     5881 caacggctct acggcgcgct ggtcgagcac cggctacgcc ccgtgaacgg aagcttcaag
     5941 atcgcccaca agcgcgtgga cctggtgaat tccgaaggcg agctcgacgg catcgcgatc
     6001 ctcttttgac gaaggtcttc cgcgactacg accagaaggc gctcgacgac cagtacgagc
     6061 agcgcatctg ggttccgcac gccgacgaga tcatcaaacg ttacgcacgg aaaagcgacg
     6121 aggtgcgttc ccggctgggt gaacccaggg ttgagaagta cggatccacc gcggtcgaaa
     6181 cactcgacat ctacggcagc ggccaaaagg ccttcgtgtt cgtgcacggc ggcgcctgga
     6241 agcgccagtc gagccgcgag caggcgtttc ccgccgaagc gatcatcgag gcgggcgccg
     6301 cgtacgtcgc gctcaacttc gcgaacctgc ccacagtgac gctgcccgag atggcggcgc
     6361 aggtccgccg cggcatcgag tgggtgcgga agaacttgag cgactacgtg gtgctgtgcg
     6421 gccactcctc tgggggccat ctctcggctt gcgccctgac aaggctagat cttgtgaaga
     6481 aggccctcgt ggtgtcgggc gtctacgatc tgctgccggt acgcctgagt gcgcgcaacg
     6541 agtatgtgcg gctcgacgag cggctcgagc acgagtattc gccgatccgg cacgtggaca
     6601 ggatccgttg cccggtgacg gtggcctggg gcgagaaaga gggtgccgag ttcttccgcc
     6661 aatcgaagga gttcgcggag aagctgggcg cgcccacgat cctggggaaa ggcctgaacc
     6721 acttcgaaat catcgaaacg cttggcgatc cgcgctccgc gctcggccgc gccgcgctta
     6781 acatgctgca atgaaggcgc ggaacgagaa gccggtcgtg atcgccggcg gcggcatcgg
     6841 cggcctcgcc gcggcgctgg cgctggcgcg caagggcttc cgctcggtgg tgctggagca
     6901 agctccccag ttcggcgaga tcggtgccgg catccagatc gcgccgaatg cgtggcacgc
     6961 gctcgacgcg ctcggcgtcg gcgccctcgt gaagaaggaa gcggtgttcg tcgagcacct
     7021 gttcatgttc gacggcgtga gcggcgagaa ggtgatcgac attccgctcg acaagcgctt
     7081 cgccaagcgc ttcggcaacc cctacgcggt gacgcatcgc gccgacatcc acggctcgct
     7141 ccttggcggc tgcacggcgc tgccggagat gatcgagctg cgaacgagca cccgcgtcac
     7201 ggcgttcgag caggacggcg cgtcggtccg cgtgaaaacg gacaacggaa gcatcgaagg
     7261 cacggcgctg gtcggggcgg acggcgggcg ctcggtgatc cgcgagaaga tcgtggccga
     7321 cgcggcgatg ccggtctccg gccacatgtg ctaccgcgcg gtgctgaaca tcggcgacgt
     7381 gccgaaagcg cttcggctgc cggcggcaac gctctgggcg gggcacaaca cgcacatcgt
     7441 tcactatccg ctgcgcggct ggaagctgtt caacctggtc gcgaccgtca tcggcaagca
     7501 ctcgagcggc ggacacaacg agctcgcgct gccggaagaa gtcttgccgc tgttcacgca
     7561 ctactgcgag aagccgacca agctgatgcg cacgccgagg gaattccggc gctggatgct
     7621 ggcgtaccgc gagccggtgg acaactggac ccggggccgc gtcacgctgc tcggcgacgc
     7681 cgcccacttc atgctgcagt acatggcgca gggcgcggcc atggcgatgg aggacgcggt
     7741 gtgcctgggt gcgtgtgcgg acgaggccga cggccagttc gagaaagcct tttcaaacta
     7801 ccaggaccga cgcatcgttc gcgccacgcg ggtgcaggtt tcggccaacc agctcgtcgg
     7861 aatgatcttc cacgtgcccg acggactgga gcgcgaagtg cgcaacgaca tctaccgcgg
     7921 caggtcggcc gagcgctact acgacgcgct cgagtggatc ttctcggcgc cggagtacgt
     7981 tcgaaacttc agtaagaaag caggtccggc ccgatctccg cgaccgatcg tgtcccgcag
     8041 agctgcatcg cgcgcaccag ctcggtcgac aggatctcga gcgcgcggcg cgcgcccggc
     8101 tcgccgcccg ccatcgcgcc gaagagcgtc gcccgcccgg taagcaccgc ctgcgcgccg
     8161 agtgcgcgcg ccttgaggat gtcgacgccg cggcgcacgc cgccgtcgag cagcacggcg
     8221 atcttcgagc cgacggcttt cgagatgtcc ggcaaggctt ccagcgtcgg cgcggcgcca
     8281 tcgagctgcc ggccgccgtg gttggaaacc accacgccgt cgcagccggc cgccacggca
     8341 cgctcggcgt cgtctgggcg ctcgacccct ttcagcagca gcttgccggg ccacagctcg
     8401 cgcaggcgct tgatgtattc ccagtcgagg ccggcgtcca tctcgcgccc caccgcggtg
     8461 acgatgtcgg tgccggcggg cgtgctgaac ttgtagccct cgaggttcgc catccccggc
     8521 aggccgtggc gcagcatctc gagcgcccag gcgggcttga agatcacatc gcgcgagttg
     8581 cgccagttgg ggctgtaggg cgtgtggaag ccgttgcggg gatcgcgctc gcgcttgccc
     8641 gacaccggca ggtccacggt caccaggatc gcctcataac ccgaattttt tgctctttca
     8701 accaagcgct tgcgaaattc ctggtcgcgt accgcgtaga gctggaacca gagccgtccg
     8761 cccacttcct gcgcgacgcg ctcgatgctg aacgccgagg gcgtggccat ggtgaaaggc
     8821 acgccgcagg cctttgcggc gcgcgcgagg atcagctcgg cgccggcgcg cccggcgctg
     8881 atgccgccgg tgggcgcgat ggcaagcggc agcgcggagg gtttgccaag cagatctacc
     8941 gtcgtctgga tttttgaaac gtcaaccagc acccgcggca gaaggcggac gcgcccgaaa
     9001 gcggcgcggt tttcgcgcag cgtcacctcg tcctcggcgc cgccgtcgaa gaagtcgaag
     9061 atggcgcgcg gcagccggcg cttcgccagc cggcgcaagt cctcgatgtt gacggcccta
     9121 tcgagattcg gcacgcggct ttgccagcgg gcaatgacgc acgcctgcga cgaatgtgca
     9181 cgagtccttc gccggcgccg gcccggcgct gaggtgatgc ggcgagacct ccggcgcagg
     9241 gggcggcggc acgaagcctg ccagcaccgc cagggcgagc cagaatccag acgtgccgga
     9301 cgagcccacg acgaccacgc ctcccgcgtg cgccgcgggc ggcagagcga ggagcaggat
     9361 gcagagaatc tggcgaactg gcggcataat tggcggctca ctctaacgga ccgacatgaa
     9421 accaggcaac aaggtcaagc gcatagaggg cgtgctatcg cccgtcatca cccctttcaa
     9481 gaaggattat tcacccgacg ccgagcgctt cgtgcgccac tgccgctggc tgctcaagtc
     9541 ggggtgcgcg ggcctcgcgg tgttcggcac caacagcgaa gcgaactcga tgtccgtctc
     9601 tgaaaagagg catctgttgg aagcattggt caaaggcggc gtgccggctt cggcgctgat
     9661 gccgggcacc gggcattgcg cgctttccga ctcggtgaaa atgacgcgcg aggcggtgcg
     9721 ccttggctgc ggcggcgtgc tgatgctgcc gccgttctac tacaagggcg tgtccgacga
     9781 gggcctctac cgcaacttcg ccgaggtgat cgagcgcgtc ggcgacgagc gcctgtcgct
     9841 gtacctctat cacatcccgc cggtcgccca ggtggcgatc acgctgaacc tgatcgagaa
     9901 actgctctcg aaatatccgg gcatcgtcgc gggcgtgaag gactcctccg gcgagtggtc
     9961 gaacaccaag gcgatgctcg acaactacgc gaagggcggc ttcgacgtgt tcgccggcag
    10021 cgaggtgttc ctgctcgaca acatgcgcaa cggcggcaag ggctgcatca ccgccaccgg
    10081 caacgtcaac ccgggcccga tcgccaacgt ctacaagaac tggcgctcga ccgaagccga
    10141 caagctgcag gccggcatca ccgcgacgcg caagatcgtg cagaagcagc cgatgatccc
    10201 ggcgctcaag gccaccgtcg cccacttcgg caacgacccg cagtggaaga cctgccggcc
    10261 gccgctcacc gagctcaccg cggcgcagga agaagaactg atccgcgacc tgaagggcgc
    10321 cgggttcagc atgcccggcc tggcggcgtg aaaacggcgc tggtcaccgg cgccgcgagc
    10381 gggattggac gtgcaacctg cgaagcattg caggttgcat tcaaggtcat tcgccttgac
    10441 cgcgaaaaga tccagggcgg c
//
LOCUS       DATBSZ010000199        39800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_102299, whole genome shotgun sequence.
ACCESSION   DATBSZ010000199 DATBSZ010000000
VERSION     DATBSZ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 39800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39800
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102299"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VIG70_12665"
     CDS             complement(<1..1148)
                     /locus_tag="VIG70_12665"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251449.1"
                     /translation="MQKNVERIAHLDLPGAGQVTVEGKHAYVGHITNKERLGTSILDV
                     SDPRQPRLLSQVFLQEKNSHSHKARVAGDLMIVNVEQNMGPAGRRAADGSPEKSSYVE
                     GGFKLYDISSREKPKLIAHHRTYGRGVHRFDMDERYAYISTEMEGYVGNILVIYDLRN
                     PARPEEVSRWWMPGQHVAGGEKPTWAGRRNRLHHALRFGDELWAGCWHAGVRVIDISD
                     IRKPRTLGEYNYHPPFPEPTHTFMPVPGRIEGRRIAVAIDEEDHAHSAEEMERRRGRP
                     HGCLWVLDVTNLEKIQPLSIFEVSELDSPWSRAAPGRFGAHQFQEHMEGTLVYCAWFA
                     GGLRIVDVADPAAPQEVGYFIPEPAAGKVAPQTNDVDVDANGLVYIVDR"
     gene            complement(1148..2098)
                     /locus_tag="VIG70_12670"
     CDS             complement(1148..2098)
                     /locus_tag="VIG70_12670"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY8251450.1"
                     /translation="MRLALLFIGLMVAGCAAAQEFPSKTVRIVVPWPPSGNVDITART
                     VAPAFGEALGQSAIVENRAGAAGAIGSAAVAKGAADGYTLLLGSSGTITAGPAVWKNR
                     QYDPVKELVAVGGIQSVPIVLTAAAKTPVSTYAEFVSLARSKQISIASAGSGSSNHLA
                     IELLMRMANLNLLHVPYKGSGPALTDLLGSQVESMMDQLTASIGHIREGRIKALAISS
                     AKRSPLLPNVPTLDEAGVKGYEAATFTGLFAPAGTPANALEKLHAALRKALASEAVRE
                     RFRGMGVEIIELAQPEFAAYVRADYEKWLKVAREGNIVVE"
     gene            complement(2101..3069)
                     /locus_tag="VIG70_12675"
     CDS             complement(2101..3069)
                     /locus_tag="VIG70_12675"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8251451.1"
                     /translation="MNWTSARSISAALALAVWTGFVHAQAYPSRAVRMLVGFPPGGGT
                     DILARVLAPRLSDYLGQPFVVENRPGATTNIASDLVAKSAPDGYTLLFTTSSLAINAS
                     LYKNLTYDALRDFAPVSVFAESPNLLVAHASAGANVKELLAQAKAKPGAMNYSSAGSG
                     TSQHLAGELFKARTGASIAHIPYKGTAASLTAVIAGEVHFSFANVPAILGHVRSGRIR
                     ALAVLAPKRSELMPEVPTMQEAGVNDVVVPVWYALLAPAATPREIVKALNDATVRAAR
                     APDLKQKLVEQGADPVGNTPEEFASMLREEVAKWAEIVKISGAKAE"
     gene            complement(3041..3991)
                     /locus_tag="VIG70_12680"
     CDS             complement(3041..3991)
                     /locus_tag="VIG70_12680"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8251452.1"
                     /translation="MRGLVALLLLMAAETAAAQSYPVRPVRLVVGFPAGGPSDVPARL
                     IAEKIRVPLGQPVIVENKTGAAGMIAASDVLSQPADGHTLLLCSYIDPLNTLLYNKVS
                     YRLEDLAPVSLVSKAYYAFTISNEVPAANLSEFVSYAKARPGTLNYGRVGAGSVTEVL
                     AKQFEKLAGISMTGVTFKGTGPALQEIVAGRVHFIVGPLSVTMPLYQGKRVKILGMTS
                     PERLTIAPEVPTLAEQGLPIVSYGWWGVCARSGMPPPVVELLNRHIVAAVASPEYRNA
                     MEKSGVIPASSTPGELGQEISKTVRETSQLFRELDIGKID"
     gene            4143..4301
                     /locus_tag="VIG70_12685"
     CDS             4143..4301
                     /locus_tag="VIG70_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251453.1"
                     /translation="MEDPKHAQGSAAPAQQSLKFRDMTLGQKTVFVLKLTVSLLSFGF
                     IYPHILID"
     gene            4365..5798
                     /locus_tag="VIG70_12690"
     CDS             4365..5798
                     /locus_tag="VIG70_12690"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY8251454.1"
                     /translation="MPNPLDLRPWLAEVEALGELREVRGADWNLELGAISELNVKKDV
                     PPALLFDEIKGYPKGFRVLTCSTSSPARLSSILRLPVQKTHRGLVEVLRGKPAQWQAE
                     APNYEPVVVSSGPALENAQKTPDLLAFPAPLWHEMDGGRYIGTGCSVVTRDLDSDWVN
                     VGTYRVQLLDKSHVALDMVPGKHGRIQYEKHKKAGKPFPVAIVLGGDPLGYLISGIEI
                     PFGMCEWNYIGSILKEPVAVIRGEVTKLPFPAASEIVLEGFVEPNDERPEGPFGEFHG
                     YYPGKAGTAPCVTVSRVYYRNNPILMGSPPAKPPNDYSYSKAVMRSALLLDALVAAGL
                     SEVKSVWAHEIGGARMFNVVSIKQRYAGHARQAGHLLSQCGVGAYMSRYSVVVDDDID
                     PANLQEVMWAVATRSDPAVDIDIIRRGMGSKNDPMFVAYPYDAPLSSKAIIDACRPWE
                     HLDEFPAVAEASKELQEKTRAKWKDIL"
     gene            complement(5918..7462)
                     /locus_tag="VIG70_12695"
     CDS             complement(5918..7462)
                     /locus_tag="VIG70_12695"
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251455.1"
                     /translation="MAAKIRIDVQRGFYPIVYVRGFAATAGEREETFYDTYYGYAATS
                     VEKRDGVPPNYLEPLVFEGQLIRFLKEYGYVDAANGGLALAMSNPANLRQNPTRSLWI
                     SRFYDVDVLSDKVRAIEDHAADLRKLVCETVPAELKKIQGVDLGPKDRDYKVILVAHS
                     MGGLVSRCLIQNLLPDPTRWIHRLVTIGSPHGGIELSAVPDLLEELIAGKGNLLGAAI
                     FKEERMRQYLKLPKAAPLNGLDGKFPEGRCLCLVGSDHDSYNVAKKFTGSHSDGLVKQ
                     SNAYIQGAYWANVHRAHSGRRGIVNSFESYENLRRFLFGDTRVKLWLEGISLKMEPPK
                     AKMTELYDFEFSLSVRGTGVYLHQRRQNPCENAMRLKRADLEKDPTLHLHTGFLDTRL
                     RSPNEPRFSHFLLAFRVAQHRVKEGFLFDTQYPERTIYSESLEARVTLAGQGTAKTAA
                     VEFRWLSDSTDLDAAESWTKAQMADGAFRIELRKSETLSAQLCIRPSGWPDKTAETAG
                     IDAALA"
     gene            7504..7995
                     /locus_tag="VIG70_12700"
     CDS             7504..7995
                     /locus_tag="VIG70_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251456.1"
                     /translation="MEHSEFVARSSANSLLWGVRGVGGAVLALWGASGLAWYALASLL
                     ALSPTLGIAAWAAAHGRYGALGWLVPGVLAFAAARPGLNLVAGSPWLACGLLGVAVSI
                     WLGPLHLLGGAAVVATWILAGAVRGSALVSLQNRLLASSDDYERLRAAGKLSFTPDRR
                     ARG"
     gene            complement(8059..8250)
                     /locus_tag="VIG70_12705"
     CDS             complement(8059..8250)
                     /locus_tag="VIG70_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251457.1"
                     /translation="MTKVAASNACACGGVLLLIFSAIAPAQWYPVAALFGGLALLVVS
                     HVLTPCLDRIAVWRKQLLK"
     gene            complement(8243..8533)
                     /locus_tag="VIG70_12710"
     CDS             complement(8243..8533)
                     /locus_tag="VIG70_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251458.1"
                     /translation="MEIALGTLTIAVLLIVNGFATREVLRDRFAERHQRMFQLVALWL
                     VPVFGAILVFAMHRKPEEPSGIYREDSDPGALGDDVATSQHVGRSIKSAADD"
     gene            complement(8535..8708)
                     /locus_tag="VIG70_12715"
     CDS             complement(8535..8708)
                     /locus_tag="VIG70_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251459.1"
                     /translation="MVRFHAGVRQGFRSGQSEEKIRHSLDLSMWEKLERSYVIGRNVN
                     RAYLEAEVDSFDE"
     gene            complement(8751..9314)
                     /locus_tag="VIG70_12720"
     CDS             complement(8751..9314)
                     /locus_tag="VIG70_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8251460.1"
                     /translation="MICVRVLVTVVVLGLAPLACANELLWTRIKTESNIVVLTRNMQS
                     GGGRMRHWDESGGCKGEARLTSVGETDARRLGELFSARSIKPFVMSSPLCRCRDTAEL
                     AFGGALIDPDLREIATADPERTGVFDRKVASLLLKHRGSAPIVFVSHRPNIELLTLEL
                     VEEGELVVGKVSDSGEIQVLGRMRLRE"
     gene            complement(9340..9585)
                     /locus_tag="VIG70_12725"
     CDS             complement(9340..9585)
                     /locus_tag="VIG70_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY8251461.1"
                     /translation="MEFVWFILIGLCAGWLAGQFMKGSGFGLVGNLIVGVVGALLGGF
                     AFHLVGLAAYGLLGQLIVATVGAVLLLALLRFVKRKT"
     gene            9652..11850
                     /locus_tag="VIG70_12730"
     CDS             9652..11850
                     /locus_tag="VIG70_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8251462.1"
                     /translation="MKFALGQSVQRLEDDALLRGAGRYTDDFAPVRAAHVCFVRSPHA
                     HAKIKSVSIDRAAKAPGVVAVLTGKDVAADGLGNIPCLIPVPGLKETPRPVLALRKVA
                     HVGDPVAMVIAETLSQAKDAAEMVEVEYEPLPAVTEARKGEVAFDIGLGESKAKVEAA
                     IAQAAHVTRLELVNNRLVANPIEPRAALAEYDAASGRTTLYTPSQGPHHLHGQIADAV
                     LKTGRDKLRVVSGNVGGAFGMKIFLYPEQPAVVWAARRLKRSVRWTGERSEGFLSDAQ
                     GRDNYSIAELATDRDGRFLALRVTTWAAMGAYLSNFGPFIPQLAAPMLSGVYKIENIF
                     LNIKGTLTNTVPVDAYRGAGRPEAIYLLERVIDVAARELGLSPEELRRRNFIKPSEMP
                     YQTPVESRYDSGDFAGILDRAMQRADWGGFAARKTISEKSGKKRGIGLGMYIERCGGG
                     PGDTVRVKVDSKEVTLYSGMQDNGQGHTTTFVQLLSHKLGVDASKIKVVQGDTDVVPA
                     EGLTGGSRFLALGGVAAVNAAAEVIEKGKQEAARRLEAAASDIEYRDGEFRIGGTDRK
                     LGLFDLGLLESTQTHAPPDYTYPNGCHICEVEVDPQTGRAAIERYTVVDDFGRAMNPK
                     LLEGQVQGGTVQGIGQALLEHAVYDAQSGQLLSGSFMDYAMPRAGDLPALDCGFHHVP
                     CKTNPLGVKGAGEAGAVGAPAAVVNAVCNALGVGHLDMPLTAEKVWRSLK"
     gene            11847..12566
                     /locus_tag="VIG70_12735"
     CDS             11847..12566
                     /locus_tag="VIG70_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY8251463.1"
                     /translation="MNFNDLVVANRILANENVVDAYGHVSLRHPEDPKRFFLARSLAP
                     ELVEPGDIMEFDLEGNPLGGDPRQPYLERFIHAAVYAARSDVMSVVHAHAEDVLPFGI
                     ASVPLKPVIHSGSFMGERVPVWDIRDRFGDTNLLVTNMAQGRDLASSLDKNNVALMRG
                     HGFVAAARSLIEVVRMSVYVPRNARVQYKALQLGQLKPLSPGEIEARNAGYKPHSPET
                     WRAWEYWATRAGCAHLLSQKP"
     gene            complement(12567..13004)
                     /locus_tag="VIG70_12740"
     CDS             complement(12567..13004)
                     /locus_tag="VIG70_12740"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8251464.1"
                     /translation="MTSPREGGCLCGAVRYRVRNAPFRTGICHCRFCQRRTGSAFGVG
                     VYFRKDDFDLLQGELESYEHRSDESSRWLKMEFCPKCGTTVTWTLELFPDGRGVAGGS
                     FDDPSWLRIERHTWTRSKQHWVPIAPGVERHEKAALQQPVKLK"
     gene            complement(13014..13277)
                     /locus_tag="VIG70_12745"
     CDS             complement(13014..13277)
                     /locus_tag="VIG70_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251465.1"
                     /translation="MPGRHLLTFELSEEGDELTIHGDPPGLRLLAERISDLAGKSETL
                     SNPSVAQLSTSVWGGNELSLEPQGTDTRLVYQVRIVARPVKRK"
     gene            13456..14076
                     /locus_tag="VIG70_12750"
     CDS             13456..14076
                     /locus_tag="VIG70_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8251466.1"
                     /translation="MHIEAVKAAYRRYARIYDVIFGAVLQPGRKAVLDALRLKPGDRV
                     LEVGVGTGLSLSLYPRDVRITGIDVSREMLERARARVARARLSNVEALLEMDAETMAF
                     PDASFDKVVAMYVVSVVPQPAKLLEELHRVCRPDGEIFIVNHFQSENRVMGALERALG
                     NVSSQIGFDPEVDLRKLVPGARNGDVSRVNLFWKMVRLRNGVSHKL"
     gene            14118..14462
                     /locus_tag="VIG70_12755"
     CDS             14118..14462
                     /locus_tag="VIG70_12755"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEY8251467.1"
                     /translation="MQTFPEVVFHDVERSAWVESYILERVQRLDRFAYGITSCRVSLT
                     KDQASHHKGNRYSVMVEARTPPHHDLAARKARVVRDMQTQLPTLINHAFGAIERQLKK
                     TSQLRRAGAPAQ"
     gene            complement(14456..16498)
                     /locus_tag="VIG70_12760"
     CDS             complement(14456..16498)
                     /locus_tag="VIG70_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251468.1"
                     /translation="MRRLLAATLLLAAGCAGLPGGDSSLERRVGAVLERRGLGVDALL
                     VIDNLLRHGPPAPRAAPPVVLRLLAEPLAAANVSRLFEQAVPASLAALDPARSQATFE
                     DLLKAYLAELAEAQRLLRAATRDFAEVDFSRGLPSPDQLLAIADAIDAPGLERANLAF
                     IDATVKFVRGLQNASALPAQGRTFESAIGTIVIGTPGNDRHGPGAALIIDPGGDDLYE
                     RSPVRGGAVSVIVDLGGNDEYRGSDLALRGFSAIVDLGGNDRYEMQGSGIAVAIAGAS
                     LLIDFAGNDAYTAKFFGQGAAAFGIGALVDLGGSDHYALEAWGQGFGVAGGLGLLWDR
                     AGNDQYAVAGVPDPFARGAGLSGAQGAAFGFRGAIAGGVGILRDDGGDDLYDAQMFAQ
                     GVGYYYGLGILWDRGGDDRYQAFRYAQGNAAHQAVGVLSDEGGDDHYTADGYAQGMGL
                     DVAVGVLVDGRGNDAYRARGGAQGAATANGFGLLADQDGDDHFDIGPDERAWGRAEWL
                     RGLPSVAVLLHASGAQFIGDGKAVPAPPENPPVAVQPPTTETCPSSDPGEALLCRVRD
                     AADLEALWDELKATLVADPATPLAGWIAIALARWPPGNAAEIAALLAKRESCNVRALA
                     LRAFPTLPAAQAGIRSSCFRLQAAARMAFVRMGDPVPADAALPLFLRAIPPQEDTY"
     gene            complement(16495..17106)
                     /locus_tag="VIG70_12765"
     CDS             complement(16495..17106)
                     /locus_tag="VIG70_12765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8251469.1"
                     /translation="MNIHRLYGKFMHQAARRGGLHVLRVFSRPLERDARSAPPPGLEL
                     RPLGAEELVSYSREPALDLREAMIREAMQRGDRCLGALDGAALVGYVWFAYAIAPHVD
                     GIWVRVPPHAVYRFKALVRPSYRGRGIAAALYDVADPIVGKPGVGTVVDCIAVQNTPS
                     IAATLKSGSRPLGTVAYWRASRWFLSRHSPSVQRLGLRFYLAP"
     gene            17147..17797
                     /locus_tag="VIG70_12770"
     CDS             17147..17797
                     /locus_tag="VIG70_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8251470.1"
                     /translation="MPADLKSIPLFEGVPEADVRAISDRAITRNYPKQAIILHEGDES
                     DSLYLVLGGRVKIYLADEHGKELILAIKGPGQYFGEMALDDEPRSASVMTLEPAQFAI
                     LSRADFKAFLLSHPAVSLQLIQNLIRVARGLNRSVRNLAMLDVYGRVARVLLELAVER
                     DGKMVIPEKLTQKEIAARVGASREMINRILRDLTTGGYVSMEDGRITINKAPPARW"
     gene            18159..18425
                     /locus_tag="VIG70_12775"
     CDS             18159..18425
                     /locus_tag="VIG70_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251471.1"
                     /translation="MRAYLGLVVHIHNKEPIMQNLYIVRTATVASLTAAFVAFGWMSM
                     QDDDLQIAPEQIVVSGMQAEPAMFFHSGIVPHTNDAEPEVYEYY"
     gene            18439..18597
                     /locus_tag="VIG70_12780"
     CDS             18439..18597
                     /locus_tag="VIG70_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251472.1"
                     /translation="MLDILFTTGQAASVMLLLYGAFLVLMPVRKARKPNPMPEDLMFM
                     PRHLQNDA"
     gene            18747..18962
                     /locus_tag="VIG70_12785"
     CDS             18747..18962
                     /locus_tag="VIG70_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251473.1"
                     /translation="MQNPYRIRTATVASLVAVMLGLSWLRAPADETPVATEMVAVSST
                     PAEPAMHFHAGFTLQANADEPELYEYY"
     gene            complement(18966..19823)
                     /locus_tag="VIG70_12790"
     CDS             complement(18966..19823)
                     /locus_tag="VIG70_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8251474.1"
                     /translation="MKFLPLLLVALVAGCAGAPKATTAARQEAAIEANRKGEAYVRHG
                     ELENAARSYREALRLSQSLEDPEGIAANAINLSIVRQRQGRFADARASLEAVLEQSNL
                     RFSNVRLAEAALRHALLDLDQNRFSGAAEWVGQAAKHCGERCPLTAAIQNVRAQLALQ
                     AGRIEEAAAAARSAQEASRAAGDRSELANALRLLGISSLQRGEAAVARAHLEQALAID
                     REIGAPRKIALDLLALGRAAALSGERDAARAYYARAMAVSEADRDNAGVTEARALLEA
                     ADKAAATGK"
     gene            complement(19820..20827)
                     /locus_tag="VIG70_12795"
     CDS             complement(19820..20827)
                     /locus_tag="VIG70_12795"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY8251475.1"
                     /translation="MEEMRTEKLTAPRRFRGIRKQVNLFLLTALIGFAGVTGLVAYKQ
                     GMFVQHTNIYFHAPDASGINKGMAVRLHGVPVGAVKDIEFADRGVRVRVGVNSEYIPR
                     LPRGSLARLTREGYVGAASIQILPGTAATDRTPVAEGDEIRFIAQKGMADMLDEVRQQ
                     LTPAFQELRRAASEMSDPGSDFRRSVRAMRELVEELPAANRELRQLVRDADRTMITLG
                     RQADAVGTQAQTTLTILSRVGTQTEEHLPVLAGKLGNTLDSLNATATQVRETTRTNGE
                     ALRELLTQAPELMRGSGELVRDTQELTTAARRTWLLRDYIEPSEMRTLPVDSFESFGK
                     R"
     gene            complement(20842..21423)
                     /locus_tag="VIG70_12800"
     CDS             complement(20842..21423)
                     /locus_tag="VIG70_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251476.1"
                     /translation="MSCVLGYEPVPDEESPERVWVEEPGDHQVSCLYKDELLAAMHEL
                     KPRNGARLVLLGADVRTLGERERRELLRRVGFVPADGGLMSSLNAWENISLPLAYHAP
                     DKLAGALAEVHALLGDLGGVDDSLLAKLPEHMTLYERRLAAYLRSLLEKPELLVVEDL
                     SAGLGPTKRRRTARFAEVYRARCPGGTFVQFED"
     gene            complement(21420..22196)
                     /locus_tag="VIG70_12805"
     CDS             complement(21420..22196)
                     /locus_tag="VIG70_12805"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8251477.1"
                     /translation="MFAVYRELEGSAYLAWHAVRRLGVLRLPPVTEELVRQIYFTGAS
                     ATGGIVLRGAFIGTLIIAYVIEVLNADAGLAVKMLLWVVLREVGPLIAAVLVIQRSGT
                     AVATELALREISGEIASLRRMRIDPYDYLVVPRVAGMALSNAALTLYVQLIAVGGGMV
                     LSSLIINVPFAEMLGMFFLLASPLDLVYSFIKSLLFGVAIAVVSCYHGLNPRGHSINA
                     VPQAAIRAVTEALLAVMLLNVVFAYLVYGVAFFGLVTAEQ"
     gene            complement(22233..22931)
                     /locus_tag="VIG70_12810"
     CDS             complement(22233..22931)
                     /locus_tag="VIG70_12810"
                     /inference="COORDINATES: protein motif:HMM:NF025175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HEY8251478.1"
                     /translation="MPYPFAHPAAVLPLARPMGRFAVPSALGIGSMLPDLWYFVPLVE
                     RSDSHSVAALFWFCVPAGLIAYALFHLALKQPLIALLSPRLGSFTCAGLPPRPWYAVV
                     ASLLVGSMTHVGWDDLTHSDSHRWLQHASTLAGSAILSWWMWRKLRRAPIAAQAPRLS
                     RFHRACVIFGLLGAMLICALWSTETAWPANFRLAFDYSALRHLLRSAGIGALQGLGAA
                     VLVYCVLFQRKILQ"
     gene            complement(22962..24251)
                     /locus_tag="VIG70_12815"
     CDS             complement(22962..24251)
                     /locus_tag="VIG70_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEY8251479.1"
                     /translation="MATKFLFVSYEGLIADIAWQVAKEGHEVKLWIQNPEERGIAEGF
                     VPKTDEWKSEVDWADVVVFDDVLGMGALAEELRGQGKAVVGGSTYTDRLEDDRGFGQQ
                     ELKAAGVSIISQENFTSFDEAVAYVQSNPNRYVIKPSGEAQNIKRLLFVGEEDDGRDV
                     IQVLQDYKRFWSDKMTEFQLQKRIMGVEVAVGAFFNGREFVTPVCVNFEHKKLFPGDI
                     GPATGEMGTSMFWSDPNKLFNATLKKMEAKLAEARYSSYIDINCIVNNNGIYPLEFTS
                     RFGYPAISIQQEGLLTPMGELLRELAAGTTTSLRARSGFQIGVRIVVPPFPYNDPETL
                     ENTSKDSVILFKTPSREGVHIEDVRTADGEWLVTGTSGVVLIVCGAGPTMRQAQRQAY
                     NRVRNIMIPHMYYRDDIGERWYEDSDRLHAWGYLREA"
     gene            complement(24267..24791)
                     /locus_tag="VIG70_12820"
     CDS             complement(24267..24791)
                     /locus_tag="VIG70_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251480.1"
                     /translation="MPGANLPVQLARLPDLADGAFRAFVRFPAGWSRPGAGHYPVAEE
                     FLVLEGDLRLNHVTWRGGGYAWIAANRIRVDSCSASGCLAFAWFAAGPRWIAGAPAEP
                     APSADTVFAHWRDAPGGKLYAGPEHRTWVAPGSQIPEGCEALDLTDLCWRAHARSETP
                     AERSSVLARVWQRG"
     gene            complement(24829..25212)
                     /locus_tag="VIG70_12825"
     CDS             complement(24829..25212)
                     /locus_tag="VIG70_12825"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY8251481.1"
                     /translation="MKTGDKLPPLEKTFTSVDLFAYGAATWDWHRMHYDVELARSKGF
                     PGAVIDGQMYGAVFARAALAWAGPRAFIRRLSFKMKSMAFAGDTLVASGSVSEVKGNI
                     AVLSQQLTKDGKLVAEASTEVRLPG"
     gene            complement(25216..25710)
                     /locus_tag="VIG70_12830"
     CDS             complement(25216..25710)
                     /locus_tag="VIG70_12830"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8251482.1"
                     /translation="MALLTDELKAWIGREAHYAAREELGRASIRYFALAMGDDNPLYV
                     DDEYAKKAGYPSVIAPPTLICETSQYAHQAPNADGYIGHEWHLPVRNCRLIRAGNDYE
                     FMRPVLPADRISVTWTLEDIVERSSSRGGTQLFVTSVARYRDAKGEVVAINRETVVVQ
                     PLGE"
     gene            complement(25725..26861)
                     /locus_tag="VIG70_12835"
     CDS             complement(25725..26861)
                     /locus_tag="VIG70_12835"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8251483.1"
                     /translation="MTLTDLKPAKFPWFDYSRYSFSLGVRSATGTYLSGHTGSEYDAA
                     AKRIVVRGTMTDQVRTAWAKIGAILESGGLGYADVVRVVEYVKPEGIERYAEADGVRR
                     EVFKGHQPVVNTVPVKALLRPEAFIEIEVTASAVPGRKETSGVVFLPTVQPVDDQGNI
                     VGAGDVAAQTRAIFERASRMLAALGVGFDRVVKTLDYITPAALAAYKGTGRVRRDHLG
                     PVYPGAAGILMPRLVHPEALIQYDFIATRDTPVAVNPGWTRYQKLTYSPAVRAGKLLF
                     MSGQGSLDPATERVVHDGDVVAQAEYTYGNVIKVLEAAGGDARNLVKTIEYVTPAALA
                     RYRDVAKVREKLLHKPWPASTGLVCEALLRPEMQIEVDPFAILD"
     gene            complement(26858..27229)
                     /locus_tag="VIG70_12840"
     CDS             complement(26858..27229)
                     /locus_tag="VIG70_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEY8251484.1"
                     /translation="MKIPSPPVNPENRPFFDAAREGKLLLGKCSACQELHYYPRAVCP
                     FCSGKTELVAAKGTGKIYSYSVMRRVAEPYAIAYVTLDEGVTMLTNMVDCELDRLRIG
                     DPVRLVFKAAEGGEMIPMFTK"
     gene            complement(27226..28392)
                     /locus_tag="VIG70_12845"
     CDS             complement(27226..28392)
                     /locus_tag="VIG70_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEY8251485.1"
                     /translation="MTIKGKACIAGAFEHPTRKAPDKSTAQLHAEVAKGALADAGLDR
                     KDVDGYFCAGDAPGLGALSMLDYLGLKVRHMDTTETGGSSYVVHVAHAAQAIATGRCN
                     VALITLAGRPRSEGSSGTQPRNYGTAAPDAAFEVPYGPATVNMYAMVAMRHMHEFGTT
                     GEQLAWIKVAASHHAQHNPNAMLREVVTVKDVLDSPMIADPLHRLDCCVVSDGGGALV
                     VTRPEIARDLKRPVVRVIGAGEAMKGQAGGRIDLTWSASAWSGAAAFDEAGIKPADIQ
                     YVSIYDSFTITVLMQLEDLGFCAKGAGGRFVSDGGLVSGSGRLPFNTDGGGLSNNHPG
                     NRGGMTKVIEAVRQLRSEAHPAVQVKNCRLALANGTGGQLGSRHGSATLILERA"
     gene            complement(28389..29957)
                     /locus_tag="VIG70_12850"
     CDS             complement(28389..29957)
                     /locus_tag="VIG70_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8251486.1"
                     /translation="MGSRRVHYLRAGSGPALALLHACPCSAKVMRPLMPVFGEKFTCL
                     AFDMPGFGLSDLLPKPNPSIEDFADALAETLDALGVEHVATYGRHTGASIAVEFAARH
                     PKRTAMALADGYAVFEKRYTDEQLEAYLEPIVPAWDGGHLLRLWARYRDQHAFWPWNN
                     QTAAGRSDADIPGADFLHRGVVEMLEAGNDYRIGYAAPFRHRALDVLADLKVPVCFGN
                     RPGDSMFLTRPLYPASAWQEVMPREFGPATLAERAILLKHPARGAPPPAPRCAPLAGR
                     TTTDYVDIGGTQLFVRFEKLEKSPLLILHHSPGSSALYDDLVLEAGNAIAVDLPGHGE
                     SDPLPGNPQDPRSLALAVLNLLEKMKLGDLRVYAHNGAAAVALELAHRRGADVVLDAP
                     AFLKDQSIASRYAPAVEPVWDGSHWLRAWNHVRDSELWWPWFERRHENVLEGGHRIDP
                     QALTIRVREAMKQPASYGPAWEAALSYPWRERIKGLEKKIRTIAAPQDVFAHLSADAI
                     KVEDNARSKAKAIQ"
     gene            complement(30007..31164)
                     /locus_tag="VIG70_12855"
     CDS             complement(30007..31164)
                     /locus_tag="VIG70_12855"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8251487.1"
                     /translation="MNFEPASEHSILRDSVREFLDRELPEARIREMDRARRIPRELWK
                     RFAAMGWQGLSVPTEYGGSGADVSTAAVFCEELARRFPSLATDWLLVSMTARVLRESG
                     TPAQKKALLPRLAAGEFLMSFGMSEPGGGTDVLALKTRATLQGNEWVVRGQKLYTSFA
                     DDADAILVLCRTDPAEGAKRARGLSLVLTPRHQPAVQVRRLELMGMRAACTCEVFLDD
                     ARAPADALVGGQGNGWRTLLASLDEERILCAAMYVGITSAALDQALQYARDRQAFGRP
                     IGAFQAVQHPLAETATELEQIRLLTAKAAWLQSNGRECATEAAMAKLAATEAAIRATD
                     RCMRVLGGFGLVEETPLERLFRDARLGPFSPISNEMVRNFVGERLGLPRTY"
     gene            complement(31161..31901)
                     /locus_tag="VIG70_12860"
     CDS             complement(31161..31901)
                     /locus_tag="VIG70_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251488.1"
                     /translation="MILQVQGLAKRFGGLRALDGVDLAVEAGEFRAIIGPNGAGKSTF
                     FNALTGLLRPDDGRVSFEGRDVTGSAPHLLARMGIGRTFQITSIFRDLTAQENVQVSL
                     LAHARRAWSVWPKAAALHAARAKELLALVGLANAFERQAGTLSHGDQKRLELAIALAG
                     EPRLLLLDEPTAGMAAQERIESITLVHRLARELGLSCVFTEHDMAVVFAVATRITVMH
                     QGRVLAEGSPAEVRAHPEVQQVYLGAGA"
     gene            complement(31898..32818)
                     /locus_tag="VIG70_12865"
     CDS             complement(31898..32818)
                     /locus_tag="VIG70_12865"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8251489.1"
                     /translation="MSGAARWPLAGGIALLALPLALSDFWLFIAIEVLAFALYAVSFN
                     VLLGYGGMLSFGHAAFFGVGGYAAALLMKKTGLSAAAAFALLPLVAMAASAAFAAVTG
                     FFSVRRSGIYFAMLTFAFQMLLYTVALKATGLTGGDDGMTGLRPPGVLAKPLAYYYFA
                     LALVAPALYVLYRLVNSPFGYTLRALRTNARRVQYIGVDVRTHQLAAFIITGAFAGLA
                     GGLFALSNGNVFPGWLNWTASATPIVMAVLGGVHTFLGPALGAAVYVVLEVLISGKTE
                     YWPLAMGIIILVLVLLMPQGLTGLARRRPA"
     gene            complement(32815..33729)
                     /locus_tag="VIG70_12870"
     CDS             complement(32815..33729)
                     /locus_tag="VIG70_12870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8251490.1"
                     /translation="MIDSFLQQLAWLQNPVVVARQLLAGVSNGMLLFLICAGLSLIFG
                     VLRIINFAHGALFMLGALIAVSLIALAPGASASFVAALIGATAGLAVLGAALEAGLLR
                     RIYRAPHEFQLLLTFALVFVLGDVAKLIWGREDHSVAVPAALDGSMRALGLTFPNYRL
                     FLIVVGVAVAVGLWIVLHRTRWGIFVRAATMDRDMLSALGVNTRRLFTSVFAAGAALA
                     GLGGALAAPIVAVGPGLHVQVIIDAFVVVVIGGMGSVLGALVASLLVGIVNAFGVLAF
                     PGLAVVLTFAVMAIVLIVRPWGLFGAPE"
     gene            33823..35034
                     /locus_tag="VIG70_12875"
     CDS             33823..35034
                     /locus_tag="VIG70_12875"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251491.1"
                     /translation="MLRTLLILALCVAPPAAQAQSREPVKLGLIDIYSGGFAFIADSI
                     RTGFQIAVDEANAAGGLNGRPFQLVTADMGNSVEKAVTEGRRMILEEKIKFVTVGIHS
                     GAAVAVGNLGKEHKVLINGGFATTKRLTGEAGHDYVARANLSTVEIGRVMAEHLKSRS
                     DIKRVSVISPDYEYGQHFAADFIAGLKTARPDIAIVRQEWPKLGATDFGPHVTALQSQ
                     PTDLVVAGMFGADLVNFLRSAKDFGLFSDKTQFMVHGLDLAKMGALKDSLPPNTMGTV
                     WYPFYAIDSAKSKAFAAEVEKRMKTYATGSTTVGYVAGKMLTEAIRKAGTDDVEKAIK
                     ALGTVQFEGPTGPTKVRSCDNMALYNFYVGTVKRDAALPDGIGLGDVKAVNTETVARS
                     CADVKKARSGG"
     gene            35041..35748
                     /locus_tag="VIG70_12880"
     CDS             35041..35748
                     /locus_tag="VIG70_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251492.1"
                     /translation="MALLEVRGLETAYGASQVLFGISLEVERGKVVALLGRNGAGKTT
                     TLASVMGLVRPRAGSIRFDGAEILGREPFEACRAGLGFVPENCRLFAGLTVAENFEAA
                     RRPTQAGEGRWDLDEVLDLFPDVRSFLGQRAGLLSGGQQRMVAIARTLLGNPKLLLLD
                     EPSEGLAPLVVDAILQRLKRLKASGTTVLISEQNLRFATELADRVSIIERGEIRYEGT
                     PAELAARPEVREKYLMV"
     gene            complement(35745..36842)
                     /locus_tag="VIG70_12885"
     CDS             complement(35745..36842)
                     /locus_tag="VIG70_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY8251493.1"
                     /translation="MSDLFSPLAIGDLALPNRIVMAPLTRSRATPERVPTPMMIEHYV
                     QRASAGLIIAEATNVVAQSNAWECAPGIFTPAQIDAWRPLVAAVHAAGGRIVLQLWHG
                     GRVAADRSPKPVPALSPSGVNENLEAITVWGRGDDGKFIKLKATPSRAMTLEEIRGTV
                     AAFGEAARACREIGFDAIELHGANGYLPHQFLSPFVNRRTDDYGGTPEKRARFAIEVV
                     QAMMKHYPPARVGMRISPYTDYNGAVDPDPSPAFRFLVKELNQSGLGWLELADTSFWW
                     GKFERTRMLELVRPVFDGKIIANGGIDPDTANQLIGAGAVDAVSFGRLWMANPDLPER
                     IRRGGPYAKAITKRFYGGGPDGYNDYPTLPG"
     gene            complement(36850..38394)
                     /locus_tag="VIG70_12890"
     CDS             complement(36850..38394)
                     /locus_tag="VIG70_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8251494.1"
                     /translation="MNLAHTLTQSAANWPDKTALVFECRRWTYAQWNRSVNKAAHAFR
                     AAGIGKGERVAFLTYNLPEQVTGFYALMKIGAVPVPINYRLAANEVKYIVDDSRARIL
                     VFEEALRAPVAAIQDRLSVSQLIYVGAKPEGKEIPFEKFIAPGSEAEPEAEVGWHDPA
                     FIMYTSGTTGRPKGVVRSHLAEIMGSHSMQVECGFRADDTILNNKPLFHIAQLQLQFI
                     PFVHLGGTNVLTRGFDVHETLSQVGAERITVLHGVPTQMVMMAGADLTKYDLRSLRRG
                     FFGGQTLADDVTRKVMAWFPEYFANVYGSTEALMVTCCDYRKYPGKLGSVGKASVNMA
                     VRVIRTDSREPDDLCKPGEIGQLITRGPSLLTEYFGLPERTAAAFAKGWFLSGDAAVL
                     DDEGFITVMGRMDHTIKSGGENIHPSEIENVLFKHPGIANAAAVGLPSRKWGQVVCAA
                     VVRKDPALSAAALDEFCLKSPELADFKRPRHYFFIDAIPSNPTGKVERGKLKDQLMAG
                     LPGELE"
     gene            complement(38404..39600)
                     /locus_tag="VIG70_12895"
     CDS             complement(38404..39600)
                     /locus_tag="VIG70_12895"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8251495.1"
                     /translation="MTPPLAGLRILAVEQYGAGPAGTLYLADLGAEVIKIENHKDGGD
                     VGRHVGPHFFGPGDSQFFQTFNRNKKSVTLDLKHPEGKAAFRALVKTADAVLDNLRGD
                     HPEKLGLTYEDLKQVNPRIVCAHLSAYGRTGSRKAWPGYDYLMQAEAGHMSLTGEPGG
                     PPTRYGLSIVDLMTGLAAAFGLLSGVLSARATGKGMDVDTSLFDVALHNLNYPGTWFL
                     NAGEVTGRTPRSGHPSLTPSQLYRTRDGWIFIMCNKEKFWGVLAQALGKPEWATDPDF
                     ATFKARLANRERVTQMLDAALMTRTTAEWIERFAGNVPASPVYDVKQALESEFVAERG
                     GVAEFAYPAGGSARMVAAPIRFPGVELPTRAAPPMGADTVEQLRAAGYSDERISTLRA
                     AGVISG"
     gene            complement(39597..>39800)
                     /locus_tag="VIG70_12900"
     CDS             complement(39597..>39800)
                     /locus_tag="VIG70_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY8251496.1"
                     /translation="VAVNRGNPDRPLTNAEIEAKYFENCALTLEESAAKRIRNAVLDL
                     ESIEDAAELEALLAADFALEAVT"
BASE COUNT         6375 a        13369 c        13773 g         6283 t
ORIGIN      
        1 cggtcgacga tgtagacaag gccgttcgcg tcgacgtcca cgtcgttggt ctgcggcgcg
       61 accttgccgg ccgccggctc cggaatgaag tagccgacct cctgcggcgc cgcggggtcg
      121 gcgacgtcga cgatgcgcag cccgccggcg aaccaggcgc agtacacgag cgttccttcc
      181 atgtgctcct ggaactggtg cgcgccgaag cggccgggcg cggcgcggct ccacggcgag
      241 tcgagctcgc tcacctcgaa tatcgacagc ggctggattt tttcgaggtt ggtgacgtcg
      301 agaacccaca agcagccgtg cgggcggccg cggcgccgct ccatctcctc tgccgagtgc
      361 gcgtggtctt cctcgtcgat cgccacggcg atgcggcggc cttcgatgcg gccgggcacc
      421 ggcatgaagg tatgcgtggg ctcggggaag ggcgggtggt agttgtactc gccgagggtg
      481 cgcggcttgc ggatgtcgga gatgtcgatg acgcgcacac cggcgtgcca gcagcccgcc
      541 cagagctcgt cgccgaagcg cagcgcgtgg tgcaggcggt tgcgccgccc ggcccaggtc
      601 ggcttctcgc cgcccgcgac atgctgtccc ggcatccacc agcgcgacac ttcctccggc
      661 cgcgcgggat tgcgaaggtc gtagatcaca aggatgttgc cgacgtagcc ctccatctcg
      721 gtggagatgt aggcgtagcg ctcgtccatg tcgaaacggt gcacgccgcg gccgtaggtg
      781 cggtggtggg cgatgagctt cggtttttcc ctgctcgaaa tatcgtagag cttgaagccg
      841 ccttcaacat aagaggattt ctcaggtgaa ccgtcggctg ctctgcggcc ggccggcccc
      901 atgttctgct cgacgttgac gatcatcagg tcgccggcga cgcgcgcttt gtggctgtgg
      961 gaattttttt cttgaagaaa aacctgactg agcagacgtg gctggcgcgg atccgagacg
     1021 tcgaggatcg aggtgccgag gcgctccttg ttggtgatgt gcccgacgta ggcgtgcttg
     1081 ccctcgacgg tgacctggcc cgcgcccggc agatcgaggt gcgcgatgcg ttcgacgttc
     1141 ttctgcatca ctcgacgacg atgttgccct cgcgcgccac cttgagccac ttctcgtagt
     1201 ccgcgcgcac gtaggcggcg aattcgggct gggcgagctc gatgatctcg acgcccatgc
     1261 cgcggaagcg ctcgcgcacc gcttcgctgg cgagcgcttt gcgcagggcg gcgtgcagct
     1321 tttccagcgc attggccggc gtgcctgcgg gcgcgaacag gccggtgaag gtcgccgcct
     1381 cgtagccctt gacgccggcc tcgtcgagcg tcggcacgtt cggcagcaac ggcgagcgct
     1441 tcgccgagga gatcgcgagc gccttgatgc ggccctcgcg gatatggccg atcgaggcgg
     1501 tgagctggtc catcatgctc tcgacctggc tgccgagaag gtcggtgagc gccggcccgc
     1561 tgcccttgta aggaacgtgg agcaggttca ggttcgccat gcgcatcagg agctcgatgg
     1621 cgaggtggtt ggacgagccg ctgccggccg aagcaatgct gatctgcttt gacctcgcga
     1681 gagaaacgaa ctcggcgtag gttgaaacgg gagtcttggc ggctgcagtc agcacgatcg
     1741 gcaccgactg gatgccgccg accgccacca gctccttcac cgggtcgtac tgccggttct
     1801 tccacaccgc cgggccggcg gtgatggtgc cgctcgagcc gaggagcagc gtgtagccgt
     1861 cggcggcgcc tttcgccacc gccgcgctgc cgatcgcgcc ggcggcgccg gcacggttct
     1921 ccacgatcgc gctctggccg agcgcctcgc cgaaggccgg ggcgacggtg cgcgcggtga
     1981 tgtcgacgtt cccgctcggc ggccagggta cgacgatgcg cacggtcttc gagggaaact
     2041 cctgcgctgc ggcgcagccg gcaaccatca agccgatgaa gagcaacgcc aggcgcatcg
     2101 tcactccgcc ttcgcgccgg aaatcttgac gatctcggcc cacttcgcca cttcttcgcg
     2161 cagcatgctg gcgaattctt ccggcgtgtt gccgaccggg tcggcgccct gctcgacgag
     2221 cttctgcttg aggtcgggtg cgcgcgcggc gcgcaccgtg gcgtcgttca gggctttgac
     2281 gatctcgcgc ggcgtcgccg cgggcgcgag gagcgcgtac cacaccggca ccacgacgtc
     2341 gttcacgccg gcttcctgca tcgtcggcac ctccggcatc agctcggagc gcttcggcgc
     2401 gaggactgcg agggcgcgga tccgcccgct gcgcacgtgg ccgaggatcg ccgggacgtt
     2461 ggcgaagctg aagtgcactt cgcccgcgat cactgcggtc agcgacgccg ccgtgccttt
     2521 gtacggaatg tgcgcgatgg acgcgccggt gcgcgccttg aacagctcgc ccgccaggtg
     2581 ctggctcgtg cccgagcccg cagacgaata gttcatcgcg ccgggcttgg cctttgcctg
     2641 cgcgagcagc tccttcacgt tggcgccggc gctcgcgtgc gcgaccagca ggttcgggct
     2701 ctcggcgaac accgagaccg gcgcgaaatc gcgcagcgcg tcgtacgtca ggtttttgta
     2761 gagcgaagcg ttgatcgcca gcgaagaagt cgtgaagagc agcgtgtacc cgtcgggcgc
     2821 gctcttcgcc accaggtcgc ttgcgatgtt ggtcgtggcg ccgggccggt tctcgaccac
     2881 gaacggctgg ccgaggtaat cggagagccg cggcgcgagc acccgcgcca ggatgtcggt
     2941 gccgccgccc ggcgggaagc cgaccagcat gcgcaccgcg cgcgagggat aagcctgggc
     3001 atggacgaag ccggtccaga ctgcgagcgc cagcgcggcg ctaatcgatc ttgccgatgt
     3061 ccagttcacg gaacaattgc gaggtctcgc gcaccgtctt ggagatttcc tggccgagct
     3121 caccgggcgt ggaggaggcc gggatcacgc cgcttttctc catcgcgttg cggtattcag
     3181 gcgaagcgac cgcggcgacg atgtggcggt tgagcagctc gaccaccggt ggcggcatgc
     3241 ccgagcgggc gcacacgccc caccagccgt agctcacgat cggcagcccc tgctcagcga
     3301 gcgtcggcac ttccggcgcg atcgtcagcc gctcggggct ggtcatgccg aggatcttca
     3361 cccgcttgcc ctggtacagc ggcatggtga ccgacagcgg cccgacgatg aagtgcacgc
     3421 ggccggcgac gatctcctgc agcgccgggc cggtgccctt gaacgtgacg ccggtcatgc
     3481 tgatgccggc gagcttctcg aactgcttcg ccagcacctc ggtgaccgag ccggcgccga
     3541 cgcgcccgta gttgagcgtg ccgggccgcg ccttcgcgta gctcacgaac tcggaaaggt
     3601 tcgcggccgg gacctcgttg gaaatggtga acgcatagta ggccttcgac accagcgaca
     3661 ccggcgccag gtcctcgagc cgatacgaga ccttgttgta caggagcgtg ttgagcggat
     3721 cgatgtacga gcagagcagc agcgtgtgac cgtcggcggg ctgcgagagc acatcgctcg
     3781 ccgcgatcat ccctgcggcg ccggtcttgt tctcgacgat caccggctgg ccgagcggca
     3841 cgcggatctt ctcggcgatg aggcgcgccg gaacgtcgct cggcccgccg gcgggaaagc
     3901 ccaccaccag ccgcaccggg cgcacgggat agctctgcgc cgcagcagtc tcggcagcca
     3961 tgagcagcag aagcgcgacg aggcccctca tggccggcaa tggtacgtcg attgtcagac
     4021 cgctgacagg cagcgcagcg aacgaccggc tagactgcgt ctgggctcat ccggcggctt
     4081 gctcccgcgg cgaggcttgc ctcgccggca ccggctgagc tgtcctaacc aaaggagatc
     4141 ccatggaaga cccgaagcac gcgcagggga gtgcggcgcc ggctcagcag tcgctcaagt
     4201 tccgcgacat gacgctgggt cagaagaccg tatttgtcct gaaactgacc gtgtcgttac
     4261 tgtcgttcgg attcatctat ccgcacatct tgatcgactg acggcggttc acgccggaga
     4321 agggccgcga tgagcggccc tttttatttg tagactagga ccggatgccc aatccgctcg
     4381 atctgcgccc gtggctggca gaagtcgaag cgctcggcga gctgcgcgag gtgcgcggcg
     4441 ccgactggaa cctcgagctc ggcgcgatca gcgagctcaa cgtcaagaag gacgtgccgc
     4501 cggcgctgct cttcgacgag atcaagggct acccgaaagg cttccgcgtc ctcacctgca
     4561 gcacgagcag cccggcgcgc ctgtcctcga tcctgcgctt gccggtgcag aagacgcatc
     4621 gcgggctggt cgaggtgctg cgcggcaagc cggcgcaatg gcaggccgag gcgccgaact
     4681 atgaaccggt cgtcgtctcg tccgggcctg ctctggaaaa tgctcagaaa accccggacc
     4741 tgctggcatt tccggctccg ctgtggcacg agatggacgg cgggcgctac atcggcaccg
     4801 gctgctccgt ggtgacgcgc gatctggatt ccgactgggt gaacgtcggc acgtaccgcg
     4861 tccagctgct cgacaagagc cacgtcgcgc tcgacatggt gcctggcaag cacgggcgca
     4921 tccagtacga gaagcacaag aaggcgggca agcccttccc ggtggcgatc gtcctgggcg
     4981 gcgatccgct cggctatctc atctcgggca tcgagattcc gttcggcatg tgcgagtgga
     5041 actacattgg gtccatattg aaagaaccgg ttgcagtgat tcgcggagaa gtgacaaagc
     5101 ttccatttcc cgccgcgtcg gagatcgtgc tcgaaggctt cgtcgagccg aacgacgagc
     5161 gccccgaggg gccgttcggc gagttccacg gctactaccc cggaaaggcg ggcaccgctc
     5221 cctgcgtcac ggtctcaagg gtctactaca gaaacaaccc catcctcatg gggagcccgc
     5281 cggccaagcc acccaacgac tactcgtact ccaaagcggt gatgcgctcg gcgctcctgc
     5341 tcgacgcgct ggtggcggca gggctctcgg aagtgaagtc ggtgtgggcg cacgagatcg
     5401 gcggcgcgcg catgttcaac gtggtttcga tcaagcagcg ctacgcgggc catgcgcgcc
     5461 aggccggcca cctcctcagc cagtgcggcg tcggcgccta catgtcgcgc tactcggtgg
     5521 tggtcgacga cgacatcgac ccggccaacc tgcaggaagt gatgtgggcg gtagcgacgc
     5581 gcagcgatcc cgcggtcgac atcgacatca tccgccgcgg catgggatcg aagaacgacc
     5641 cgatgttcgt cgcttatccg tacgacgcgc cgctcagctc gaaagcgatc atcgacgcct
     5701 gccgcccgtg ggagcacctc gacgagtttc cggcggtggc cgaggcgtcc aaggagttgc
     5761 aagaaaagac gcgcgcgaag tggaaagaca tcctgtaggc cgctagggaa actctgaaat
     5821 agcggcaagt ggtcgttccc gcgaaggcgg gaacccatgt tttgcaaaaa gaaaaccgga
     5881 cccccgcctt cgcgggggcg acttccccag acctttccta ggcgagcgct gcgtcgattc
     5941 cggctgtctc ggccgtcttg tcgggccagc cgctcggccg gatgcaaagc tgcgccgaga
     6001 gcgtctcgct tttgcgcagc tcgatgcgga acgccccgtc ggccatctgc gccttggtcc
     6061 agctttccgc ggcgtcgagg tcggtcgagt cggacagcca gcggaactcc accgcggccg
     6121 tctttgccgt gccctggccg gcgagcgtca cgcgcgcctc caggctctcg ctgtagatcg
     6181 tgcgctcggg gtactgcgtg tcgaacagga agccctcctt gacgcggtgc tgcgcgacgc
     6241 ggaacgccag caggaaatgc gagaagcggg gctcgttcgg cgagcgcagg cgggtgtcga
     6301 ggaagccggt gtgcaggtgc agggtcgggt ccttctccag gtccgcgcgc ttcagccgca
     6361 tcgcgttttc gcacggattc tgccggcgct ggtgcaggta cacgccggtg ccgcgcaccg
     6421 acagcgagaa ctcgaagtcg tagagctcgg tcatcttggc cttgggcggc tccatcttca
     6481 gcgagatgcc ctcgagccag agcttcacgc gcgtgtcgcc gaacaggaag cggcgcaggt
     6541 tctcgtagct ctcgaagctg ttgacgatgc cgcggcggcc ggaatgcgcg cggtggacgt
     6601 tggcccagta ggcgccctgg atgtaggcgt tgctctgctt gaccagcccg tcgctgtggc
     6661 tgccggtgaa tttcttcgcg acgttgtagc tgtcgtggtc gctgccgacc aggcacaggc
     6721 agcggccctc gggaaacttg ccgtcgaggc cgttgagcgg cgcggccttc ggcagcttga
     6781 ggtactggcg catgcgctct tccttgaaga tcgcggcgcc cagcaggttg cccttgccgg
     6841 caatcagctc ctcgaggagg tcgggcaccg cggagagctc gatgccgccg tgcggcgagc
     6901 cgatcgtcac gaggcgatgg atccagcgcg tcggatcggg cagcaggttc tggatcaggc
     6961 agcgtgacac gaggccgccc atcgagtgcg cgaccaggat gaccttgtag tcacgatcct
     7021 tcgggccgag gtcgacgccc tggatcttct tcagctccgc ggggacggtc tcgcacacca
     7081 gcttgcgcag gtcggcggca tggtcttcga tcgcgcgcac cttgtcggac agcacgtcca
     7141 cgtcgtagaa gcggctgatc cagaggctgc gcgtcgggtt ctgccgcagg ttcgcggggt
     7201 tgctcatcgc gagcgccagg ccgccgttgg ccgcgtcgac gtagccgtat tccttgagga
     7261 agcggatcag ctgcccctcg aacaccagcg gctcgaggta gttcggcggc acgccgtcgc
     7321 gcttctccac cgaggtcgcg gcgtagccgt aataggtgtc gtagaaggtc tcctcgcgct
     7381 cgcccgcggt ggcggcgaag ccgcgcacgt agacgatcgg atagaacccg cgctgaacgt
     7441 cgatgcggat cttcgcggcc atggactcgc tccctcgcgg ctactctaca cttcgggccg
     7501 gggatggagc acagcgaatt cgtagcccgg agctcggcaa actcgctcct ctggggcgtg
     7561 cgcggcgtcg gcggcgcggt cctcgcgcta tggggcgcga gcggtctcgc gtggtatgcg
     7621 ctggcgtcgc ttctcgcgct gtcgccgacg ctcgggatcg cggcgtgggc cgcagcgcac
     7681 gggcgctatg gcgcccttgg ctggctggtt ccgggcgtgc tcgccttcgc cgcggcgcgg
     7741 ccgggcctga atctcgtcgc cggctcgccg tggctcgcgt gcggactcct cggggttgcg
     7801 gtctcgatct ggctgggccc gcttcacctg ctgggcggcg cggcagtcgt cgcgacctgg
     7861 atccttgccg gcgccgtcag aggaagcgct ctcgtcagcc tgcagaaccg cctgctcgcc
     7921 tcgagcgacg actacgagcg gttgagggcg gccgggaagc tgagcttcac tcccgaccgc
     7981 cgcgcgcgcg gatgaggctc gcccagccga gcgcgatggc gtcgttctcc aggtcgagcg
     8041 cccacacgtg cagctcggct acttgagaag ctgctttcgc catacggcga tccggtcgag
     8101 gcagggcgtc agtacgtgcg acacgacgag cagggcgagg ccgccgaaca gcgccgcgac
     8161 gggataccac tgggccggtg cgattgccga gaagatcagc agcagcacgc cgccgcaggc
     8221 gcacgcattc gacgccgcga ccttagtcat ccgccgcaga ttttatcgac cggccgacat
     8281 gctgagacgt tgcgacgtca tcgcccagtg cgccgggatc ggaatcctcg cggtagatcc
     8341 ccgacggctc ctcgggcttg cgatgcatcg caaaaaccag gatcgcgccg aacaccggca
     8401 cgagccacag cgcaaccagt tgaaacattc tctgatggcg ctcggcgaac ctgtcccgca
     8461 gcacttcgcg ggtcgcgaag ccgttgacga tcagcaggac cgcgatcgtg agcgttccca
     8521 gcgcgatttc cacgtcattc atcgaagctg tccacctcgg cttcaaggta cgcgcgattg
     8581 acgtttctgc cgatgacata cgaccgctcg agcttctccc acatcgaaag atcgagcgaa
     8641 tggcggatct tctcctcgct ctggccgctg cgaaagccct gcctgacccc ggcatggaac
     8701 cggaccatgg cgttgcgcaa ggcggcgacg tcgtcgatgc gggttctgct tcactcccgc
     8761 agccgcatcc ttcccaggac ctggatctcg ccggaatcgg aaaccttgcc gaccacgagc
     8821 tcgccttcct ccacgagctc cagggtcagc agctcgatat tcgggcgatg actcacgaaa
     8881 acgatgggcg cgctcccacg gtgcttgagg agaaggctgg ccaccttgcg gtcgaacact
     8941 cccgtgcgct ccgggtcggc ggtcgcgatc tcgcgcagat cggggtcgat cagcgccccg
     9001 ccgaacgcga gctcggcagt gtcgcggcag cggcacaggg gcgagctcat gacgaagggc
     9061 ttgatcgagc gtgcggaaaa cagctcgccc agccttcgcg cgtcggtctc gccgaccgag
     9121 gtgaggcggg cttcgccctt gcagccgccg ctttcgtccc agtgccgcat tctgccgcca
     9181 ccgctttgca tgttccgcgt gaggaccacg atgttggatt ccgtcttgat gcgggtccaa
     9241 agcagctcgt tggcgcaggc cagcggcgcg agaccgagaa caacgaccgt caccaggacg
     9301 cgaacgcaga tcatcgcgtg tcgccctcgg tgtgccggcc tacgtctttc gcttgacgaa
     9361 gcgaaggagc gcgagcagga gaaccgcgcc cacggtcgcc acgatcagtt gcccgaggag
     9421 gccgtaggcg gccaggccga cgaggtggaa cgcgaagccg ccgagcagcg caccgaccac
     9481 gccgacgatc aggttgccga cgaggccaaa ccccgagcct ttcatgaact gcccggcaag
     9541 ccatcctgcg cacagcccga tcaagatgaa ccagacgaac tccatgagtt acctccctgt
     9601 tagacgccgg atatcatcac tgctagtctt gatgaaggca attcgattta catgaaattc
     9661 gccctcgggc aatcggtcca gcggctcgaa gacgacgcgc tattgcgcgg cgcagggcgc
     9721 tacacggacg acttcgcgcc ggtgcgcgcg gcgcacgtgt gtttcgtgcg ctcgccgcat
     9781 gcgcacgcga agatcaagag tgtttcgatc gaccgcgccg ccaaggcgcc gggcgtagtg
     9841 gcggtgctga ccggcaagga cgtggcggcc gatgggttgg ggaacattcc gtgcctgatt
     9901 ccggtgccgg gcctcaagga aacgccgcgc ccggtgctcg cgctcaggaa ggtcgcgcat
     9961 gtcggcgacc cggtggcgat ggtgatcgcg gagacgctct cgcaggcgaa ggatgcggcg
    10021 gagatggtgg aggtggagta cgagccgctt cctgcggtca ccgaagcgag gaagggcgag
    10081 gtcgcgttcg acatcggcct cggcgaaagc aaggcgaagg tcgaagcggc gatcgcgcag
    10141 gccgcccatg tgacgcggct cgagctggta aacaaccgtc tggtggcgaa tccgatcgag
    10201 ccgcgcgcgg cgctcgccga gtacgacgcg gcgagcgggc gcaccacgct ctacacgccg
    10261 agccagggcc cgcaccatct gcacgggcag atcgccgacg cggtgctgaa gaccgggcgc
    10321 gacaagctgc gcgtggtgtc gggcaacgtc ggcggcgcgt tcggcatgaa gatcttcctc
    10381 tacccggagc agcccgccgt ggtgtgggcc gcgcggcggc tcaagcgctc ggtgcgctgg
    10441 acgggcgagc gcagcgaggg atttctttcc gacgcccagg gccgcgacaa ttactcgatc
    10501 gcggagctcg cgaccgatcg cgacgggcgc ttcctcgcgc tgcgcgtcac gacctgggca
    10561 gccatgggcg cgtacctgtc gaacttcggc ccgttcattc cgcagctcgc cgcgccgatg
    10621 ctttccgggg tttacaagat agaaaacatt ttcttgaaca tcaaaggcac gttgacgaac
    10681 accgtgccgg tcgacgccta ccgcggcgcg ggccggcccg aggcgatcta tctcctcgag
    10741 cgcgtgatcg acgtcgcggc gcgcgagctc ggcctctcgc ccgaggagct gcggcggcgc
    10801 aacttcatca agccctcgga gatgccttac cagacgccgg tcgagagccg ctacgactcg
    10861 ggggatttcg cggggatcct ggaccgggcg atgcagcggg cggattgggg cgggtttgcc
    10921 gcgagaaaga cgatctccga gaagtcagga aaaaagcgtg ggatcggcct gggcatgtac
    10981 atcgagcgtt gcggcggcgg gccgggagac acggtacggg tgaaggtcga ctcgaaggag
    11041 gtgacgctct attcaggcat gcaggacaac gggcaggggc acacgacgac cttcgtgcag
    11101 cttctttcgc acaagctggg ggtcgacgct tcgaagatca aagtcgtaca gggcgatacc
    11161 gacgtcgttc cggccgaggg tctcaccggc ggctcgcgct ttctcgcgct cggcggggtc
    11221 gccgccgtga atgcggccgc tgaggtgatc gagaagggca agcaggaagc ggcgcgcagg
    11281 ctcgaagccg cggcctccga catcgagtac cgcgacggcg aatttcgcat cggcggcacg
    11341 gatcggaagc tgggcctgtt cgatttgggt ctgttggagt caacccagac gcacgcgccg
    11401 ccggattaca cctacccgaa cggctgccac atctgcgagg tcgaggtgga cccgcagacc
    11461 ggacgcgccg cgatcgagcg ctacaccgtg gtcgacgatt tcggccgcgc catgaacccg
    11521 aagctgctcg agggccaggt gcagggcggc acggtgcagg gcatcggcca ggcgctcctc
    11581 gagcacgcgg tgtacgacgc gcagagcggc cagctccttt cgggctcgtt catggactac
    11641 gcgatgccgc gcgccggcga cctgccggcg ctcgattgcg gcttccacca cgtgccgtgc
    11701 aagaccaatc cgctcggggt aaagggcgcg ggcgaagcgg gcgcggtcgg ggcgcccgcg
    11761 gcggtggtga acgccgtatg caatgcgctc ggggtggggc atctggacat gccgctcacc
    11821 gcagaaaagg tctggcgatc gctgaagtga acttcaacga cctggtagtg gcgaaccgca
    11881 tcctcgcgaa cgagaacgtg gtggacgcgt acggacacgt cagcctgcgc cacccggagg
    11941 acccgaagcg tttcttcctc gcgcgctcgc tcgcgcccga gctggtcgag ccgggcgaca
    12001 tcatggagtt cgatctcgag ggcaatccgc tcggcggcga cccgcgccag ccctacctcg
    12061 agcgcttcat tcacgccgcc gtgtacgcgg cgcgctcgga cgtcatgtcg gtggtccacg
    12121 cgcatgccga ggacgtgctg ccgttcggca tcgcgtccgt gccgctgaag ccggtgattc
    12181 attccggcag cttcatgggc gagcgcgtgc cggtgtggga catccgcgac cggttcggcg
    12241 acacgaacct cctcgtcacc aacatggcgc agggccggga cctcgcttcg tccctggaca
    12301 agaacaatgt cgcgctgatg cgcgggcatg gcttcgtcgc cgccgcgcgc tcgctgatcg
    12361 aggtggtacg gatgtcggtc tacgtgccgc gcaatgcgcg tgttcaatac aaggcattgc
    12421 agttgggtca gctgaaaccc ctgagcccgg gcgagatcga ggcgcgcaac gccggctaca
    12481 agccgcactc gcccgagacc tggcgcgcct gggaatactg ggcgacgcgc gcgggctgcg
    12541 cgcatctcct gtcgcagaag ccctgattac ttgagcttta cgggctgctg gagcgccgcc
    12601 ttctcgtgcc gctcgacccc gggcgcgatc ggcacccagt gctgcttcga gcgcgtccag
    12661 gtatggcgct cgatcctgag ccagctcggg tcgtcgaagc tgccgcccgc gacgccgcgg
    12721 ccgtccggaa agagctcgag cgtccaggtg acggtggtgc cgcacttggg acagaactcc
    12781 atcttcagcc agcggctgct ctcgtcggag cgatgctcgt acgactcgag ctcgccctgc
    12841 agcagatcga agtcgtcctt cctgaaatag acgccgacgc cgaaggcgct gccggtgcgg
    12901 cgctggcaga acctgcagtg gcatatgccg gtgcggaacg gcgcgttcct gacgcgatag
    12961 cggaccgcgc cgcacaggca gccgccctcc cgggggctcg tcatgcggga agtctacttg
    13021 cgcttgacgg gccgcgccac gatccgcacc tggtagacga ggcgcgtgtc ggttccctgc
    13081 ggctcgagcg aaagctcgtt gccgccccag acgctggtgg agagctgggc gacgctcggg
    13141 ttgctcagcg tttcggattt gccggcgaga tcggagatgc gttcggcgag cagcctcagg
    13201 ccgggcggat cgccgtggat ggtgagctca tcgccttctt ccgagagctc gaacgtcagc
    13261 agatggcgtc ccggcacacg cacattacgc gtgcgccggg cggacctgac gatcctccag
    13321 aaggctcatg ccggggccga aactgtcggg aaaactccgc taacagggta gtccgtccgg
    13381 cctagaagga gcgttccgct tatcggcatg atcccggcgt gccgggaatg ccgattgctg
    13441 gcagaattcc ccgaaatgca catcgaagcc gtcaaagcgg cctaccggcg ctatgccaga
    13501 atctacgacg tcatcttcgg cgcagtcctg caaccgggcc gcaaggcggt tctcgatgcg
    13561 ctcaggctca agccgggcga ccgcgtcctc gaggtcggcg taggcaccgg cctgtcgctg
    13621 tcgctttatc cgcgcgacgt gcgcatcacc ggcatcgacg tgtcgcgcga gatgctcgag
    13681 cgggcgcggg cgcgcgtggc gcgcgcgcgg ctctcgaacg tcgaggcgct gctcgaaatg
    13741 gacgccgaga cgatggcgtt ccccgatgcg agcttcgaca aggtggtggc gatgtacgtg
    13801 gtgtcggtgg tgccgcagcc ggcgaagctg ctggaggagc tgcaccgggt ctgccgcccc
    13861 gacggcgaga tcttcatcgt caaccacttc cagtccgaga accgcgtcat gggcgcgctc
    13921 gagcgcgcgc tcggcaacgt ttcgtcgcag atcggcttcg atcccgaggt cgacctgcgc
    13981 aagctcgtgc ccggggcccg gaacggcgac gtgtcgcgcg tgaacctgtt ctggaagatg
    14041 gtgcgcctgc gcaacggggt gtcccacaag ctgtaaatcc ggggaacttg ccccgacggc
    14101 agggttgtcg aacagggatg caaaccttcc cggaagtcgt tttccacgac gtcgagcgtt
    14161 ccgcctgggt cgagagctac atcctcgagc gcgtgcagcg gctcgaccgg ttcgcttacg
    14221 gcatcacctc ctgccgggtc tcgctcacga aggaccaggc ctcgcaccac aagggcaatc
    14281 gctacagcgt gatggtcgag gcgcgcacgc cgccgcacca cgacctcgcg gcgcgcaagg
    14341 cgagggtggt gcgcgacatg cagacgcagc tcccgacgct catcaaccac gccttcggcg
    14401 cgatcgagcg ccagctgaag aagacgtcgc agctgcggcg cgcgggcgcg ccggctcagt
    14461 aggtatcttc ctgcggagga atcgcccgca ggaatagcgg cagcgcggcg tcggcgggca
    14521 ccgggtcgcc catgcgcacg aaggccatcc gggccgccgc ctgcagccgg aagcatgagg
    14581 agcgtattcc cgcctgcgcc gccggaagcg tcggaaaggc gcgcagcgcg agcgcgcgca
    14641 cgttgcagct ttcgcgcttc gccagcagcg cggcgatttc cgccgcattc cctggtggcc
    14701 atcttgcgag cgcgatcgcg atccatccgg ccagcggcgt cgcagggtcc gcgaccaggg
    14761 tcgccttcag ctcgtcccag agcgcttcga gatcggctgc gtcgcgcacg cgacagagca
    14821 atgcctcccc aggatcggac gaggggcagg tctccgtggt cggcggctgg accgctacgg
    14881 gcgggttttc gggcggcgcc gggaccgcct tgccgtcccc gatgaactgc gcgccgctcg
    14941 catgcagcag gacggccaca ctcggcaggc cgcgcagcca ttcggcgcgc ccccaggcgc
    15001 gctcgtcggg cccgatgtcg aagtgatcgt ccccgtcctg gtcggcaagc aggccgaagc
    15061 cgttggcggt ggcggcgccc tgggcaccgc cgcgggcgcg atacgcgtcg ttcccgcggc
    15121 cgtcgaccag cacgccgacg gcaacgtcga ggcccatgcc ctgcgcgtag ccgtcggcgg
    15181 tgtaatggtc gtcgccgcct tcgtcgctca gcacgcccac cgcctggtgc gccgcgttgc
    15241 cttgcgcgta gcggaaggcc tggtagcggt cgtcgccgcc gcgatcccag aggattccca
    15301 gcccgtagta gtagccgacg ccctgggcga acatctgggc gtcgtagaga tcgtcgccgc
    15361 cgtcgtcgcg caggatgccg acgccgcccg cgatcgcgcc gcggaagccg aaagccgcgc
    15421 cctgggcgcc actcagcccg gcgccgcggg cgaacgggtc gggcacgccc gccaccgcgt
    15481 actggtcgtt gccggcgcgg tcccagagga gcccgaggcc gccggcgacg ccgaacccct
    15541 gtccccacgc ctccagcgcg tagtggtccg agccgccgag atcgaccagc gcgccgatcc
    15601 cgaacgccgc cgccccttgc ccgaagaact tcgccgtgta ggcgtcgttg ccggcgaagt
    15661 cgatgagcag cgaggcgccg gcgatcgcca cggcgatgcc gctgccttgc atctcgtagc
    15721 gatcgttgcc gccgagatcg acgatggccg agaagccgcg cagcgcgagg tcgctgccgc
    15781 ggtattcgtc gttgccgccg aggtccacga tcacggacac cgcgccgccg cgcaccggcg
    15841 agcgctcgta gaggtcgtcg ccgcccggat cgatgatgag cgcggcgccg gggccgtgcc
    15901 ggtcgttgcc cggcgttccg atcacgatcg tgccgatggc ggactcgaag gtcctgcctt
    15961 gagccggcag cgcgctcgca ttctgcaggc cgcgcacgaa cttcacggtc gcgtcgatga
    16021 acgcgaggtt ggcgcgctcg agccccggcg cgtcgatcgc atcggcgatc gccaggagct
    16081 ggtcggggga cggcaatcca cgcgagaaat cgacctcggc gaaatcccgg gtcgcggcgc
    16141 gcagcaggcg ctgcgcttcg gcgagctcgg cgaggtaggc cttcagcagg tcctcgaacg
    16201 tcgcctgcga ccgggccggg tcgagcgcgg cgaggctcgc cggcaccgct tgctcgaaca
    16261 gcctggagac gttcgcggcg gccagcggct cggcgaggag ccgcagcacg accggcggcg
    16321 cggcgcgggg cgccggcggg ccatggcgca gcaggttgtc gatcacgagg agcgcgtcga
    16381 cgcccaggcc gcgccgttcc agcaccgcgc cgacccggcg ctccagcgaa gaatctccgc
    16441 ccggcagccc ggcgcagccg gcggcaagga ggagcgtcgc ggcgagaagc cgcctcatgg
    16501 cgcgagatag aaccgcaggc cgaggcgctg cacgctgggg gaatgccgcg agaggaacca
    16561 ccggctcgcc cgccaatagg cgacggtacc gagcggccgg ctgccgctct tgagcgtcgc
    16621 cgcgatcgag ggcgtgttct ggaccgcgat gcagtccacc acggtgccta cgcccggctt
    16681 gccgacgatc gggtcggcga cgtcgtacag cgcggcggcg atcccgcggc cgcgatacga
    16741 gggccgcacc agggccttga agcggtacac ggcgtgcggc ggcacccgga cccagatgcc
    16801 gtccacgtgc ggcgcgattg cgtaggcgaa ccagacgtag ccgacgaggg cggcgccgtc
    16861 gagcgccccc aggcaccggt cgccgcgctg catcgcctcg cggatcatcg cctcgcgcag
    16921 gtcgagcgcg ggttcccggc tgtacgaaac cagctcctcc gcgccgagcg gcctcagctc
    16981 gaggcccggc ggcggcgcgg atcgggcgtc gcgctcgagc ggccgcgaga acacgcgcag
    17041 cacatgcagc ccgccgcgcc tcgccgcttg atgcatgaac ttgccgtaga gccggtgaat
    17101 attcacgctt gtagcttaac cgcagccgcg cctagaatcc cccgccatgc cggccgatct
    17161 gaagagcatt ccgctgttcg agggcgttcc cgaagccgat gtgcgcgcga tctccgatcg
    17221 cgcgatcacc cgcaactacc cgaagcaggc gatcatcctc cacgagggcg acgagagcga
    17281 ttcgctctac ctggtcctcg gcgggcgggt aaagatctac ctggcggacg agcacggcaa
    17341 ggagctcatc ctggcgatca aggggcccgg gcagtacttc ggcgagatgg cgctcgatga
    17401 cgagccgcgc tcggcctcgg tgatgacgct cgagccggcg cagttcgcca tcctgtcgcg
    17461 cgccgatttc aaggccttcc tgctctccca ccccgccgtg tcgctgcagc tcatccagaa
    17521 cctgatccgg gtcgcgcgcg gcttgaaccg cagcgtgcgc aacctcgcca tgctcgacgt
    17581 ctacggccgc gtggcgcgcg tcctgctcga gctcgcggtc gagcgcgacg gcaagatggt
    17641 gatcccggag aagctgacgc agaaggagat cgccgcgcgc gtcggcgcct cgcgcgagat
    17701 gatcaaccgc atcctgcgcg acctcacgac cggcggctac gtcagcatgg aagacggccg
    17761 catcaccatc aacaaggctc cgcccgcgcg ctggtagtcg gaggcgccgg gcaggtgaag
    17821 atcgcacaaa cccgatgtgt cgccggcgat agcgcgcggc gcctcgcggc cgatacagtg
    17881 gttcccaggt tggcaacgaa cgaactcacg gaggggaccc aatgatcgac ctcattctca
    17941 cgataggcca ggctgccgcg gcggtgctga tgatttacgg tgccatgctc tcgatcggga
    18001 ccgctcagaa cgacgcataa gaagaacgaa ccgaaccgaa ccgaactcaa gactcctcct
    18061 ccgggccgcg aaagcggccc ttttttttca ctcccgtttt tcggcgccgg tgaattgcac
    18121 acggcggtgc tgtgcgagcg ttactttcgc cggctgcagt gcgcgcgtac cttgggctcg
    18181 tcgtccacat ccacaacaag gagccgatca tgcaaaacct ctacatcgtc cggaccgcaa
    18241 ccgtcgcctc gctgaccgcg gctttcgttg cctttggatg gatgagcatg caggacgacg
    18301 acctgcagat cgctccggag cagatcgtcg tgtcgggcat gcaggccgaa cccgccatgt
    18361 tctttcactc cggaatcgtc ccccatacga acgacgccga gcccgaagtc tacgagtact
    18421 actgagaggc cattcgccat gctcgacatc ctcttcacga ccggccaggc agcgtcggtg
    18481 atgcttctcc tctacggcgc ttttctagtc ctcatgcccg tgcgcaaggc gcgcaagccg
    18541 aacccgatgc ccgaggactt gatgttcatg ccgaggcatc tccagaacga cgcctgatcc
    18601 gaaaccgaat tcaagactcc tcctccgggc cgcgaaagcg gccctttttt tccaggtgaa
    18661 ttgcgcaccg gcgcgcggtg cgcgcgttac tttcagcgga agcgatgcgc ggataacttg
    18721 aaggctcctt cacaaaggag cccgtcatgc aaaaccctta ccggatccgt actgcaaccg
    18781 tcgcgtcgct cgtcgccgtc atgctgggct tgagctggtt gagggcgccg gcagacgaga
    18841 cgccggtagc gaccgaaatg gtggcggtat cgagcacccc ggctgagccg gcgatgcatt
    18901 tccacgccgg cttcaccctc caggcgaacg ccgacgagcc ggaactctac gagtactact
    18961 gagagctact tgccggtggc ggcggctttg tccgccgctt ccagcaacgc ccgcgcctcg
    19021 gtgacgccgg cgttgtcgcg gtcggcttcg ctgaccgcca tggcccgcgc gtagtaggcg
    19081 cgcgccgcgt cgcgctcgcc gctgagtgcg gcggcgcggc cgagggccag caggtcgagc
    19141 gcgatcttgc gcggcgcgcc gatctcgcgg tcgatggcca gtgcctgctc gaggtgagcc
    19201 cgggcaacag cggcttcgcc gcgctgcaat gagctgattc ccagcagccg cagggcgttg
    19261 gcgagctcgc tgcggtcgcc ggcggcgcgg ctcgcctcct gcgcgctgcg cgccgcggcc
    19321 gccgcttcct cgatgcggcc ggcctgcagg gcgagctggg cgcgcacgtt ctggatcgcc
    19381 gcggttagcg ggcaacgctc gccgcagtgc ttcgcggcct ggccgaccca ctcggctgcg
    19441 cccgaaaagc ggttctggtc caggtcgagc agcgcgtgcc gcagcgccgc ctctgccagc
    19501 cggacgtttg aaaaccgcag attggactgc tcgaggaccg cctcgaggct cgcgcgcgca
    19561 tcggcgaagc gcccctggcg ctggcgcacg atggaaaggt tgatcgcgtt ggcggcaatg
    19621 ccttccgggt cctcgagcga ctgcgacagg cgcagcgcct cgcggtagct gcgcgccgcg
    19681 ttttcgagct cgccgtggcg cacgtaggct tcgcccttgc ggttggcctc gatcgcggct
    19741 tcctgcctcg ctgccgtcgt ggctttgggc gcccctgcgc acccggcgac gagggcgaca
    19801 agaagtaacg gcaaaaactt catcgtttgc cgaaggactc gaagctgtcg accggaagag
    19861 tgcgcatctc ggaaggctcg atgtagtcgc gcagcagcca ggtgcggcgc gccgcggtgg
    19921 tcagctcctg cgtgtcgcgc accaactcgc cgctgccgcg catgagctcg ggcgcctgcg
    19981 taaggagttc gcgcagcgcc tcgccgttgg tccgcgtcgt ctcgcgcacc tgcgtcgcgg
    20041 tcgcgttgag ggaatcgagc gtgttgccga gcttgcccgc cagcaccggc aggtgctcct
    20101 cggtctgcgt gcccacccgg gagaggatgg tgagcgtggt ctgggcctgc gttccgaccg
    20161 cgtcggcctg gcggccgagc gtgatcatgg tgcggtcggc gtcgcgcacg agctggcgca
    20221 gctcgcggtt cgccgcgggc agctcctcga ccagctcgcg catcgccctc accgagcggc
    20281 ggaagtcgct gcccgggtcg gacatctcgg aggccgcccg gcgcagctcc tggaacgccg
    20341 gcgtgagctg ctggcgcact tcgtccagca tgtcggccat gcccttttgc gcaatgaagc
    20401 ggatctcgtc gccttcggcg accggggtgc gatcggtggc cgccgtcccc gggaggatct
    20461 ggatcgaggc ggcgccgacg tagccttcgc gcgtcagccg ggccagcgac ccgcgcggca
    20521 gccgcgggat gtactcgctg ttgacgccca cgcgcacgcg cacgccgcgg tcggcgaact
    20581 cgatgtcctt gaccgcgccc accggcacgc cgtgcaggcg caccgccatg cccttgttga
    20641 tgccgctggc gtcgggggcg tggaaataga tgttggtgtg ctggacgaac atgccctgct
    20701 tgtaggcaac caggccggtg acgccggcga agccgatgag cgccgtgagc aggaacaggt
    20761 tgacctgctt gcggatgccg cgaaagcgcc gcggcgcggt gagcttctcg gtgcgcattt
    20821 cttccatggg tggttcctcc ctcaatcctc gaactgcacg aaagtcccgc ccgggcagcg
    20881 cgcgcggtag acctcggcga agcgcgcggt gcgcctgcgc ttggtcggcc cgagcccggc
    20941 ggacaggtcc tcgaccacca gcagctccgg cttctcgagc agcgagcgca ggtacgccgc
    21001 gagccggcgc tcgtagagcg tcatgtgctc gggcagcttg gcgagcaggc tgtcgtcgac
    21061 gccgccgaga tcgccgagca gggcgtgcac ctcggcgagc gcgcccgcaa gcttgtccgg
    21121 cgcgtggtag gcgagcggca gcgagatgtt ctcccaggca ttcaggctgc tcatcaggcc
    21181 gccgtccgcc ggcacgaatc cgacgcggcg caggagctcg cgccgctcgc gctcgccgag
    21241 cgtgcgcacg tcggctccga gaagcaccag gcgcgcgccg ttgcgcggct tgagctcgtg
    21301 catcgcggcg agcagctcgt ccttgtagag gcagctcacc tgatggtcgc cgggctcctc
    21361 cacccagacg cgctccggag actcttcgtc agggaccggc tcgtagccga ggacgcagct
    21421 cattgctctg cagtaaccaa tccgaagaac gcgacgccgt agacgagata ggcgaacacc
    21481 acgttgagca gcatcaccgc gagcagcgcc tcggtcaccg cccggatcgc cgcctgcggc
    21541 acggcgttga tggaatggcc gcgcggattc aggccgtggt agcacgagac cacggcgatc
    21601 gcgacgccga acagcaggct cttgatgaac gagtacacca ggtcgagggg gctcgcgagc
    21661 aggaagaaca tgccgagcat ctcggcgaac ggcacgttga tgatgagcga cgagagcacc
    21721 atgccgccgc cgaccgcgat cagctggacg tacagcgtga gcgcggcgtt ggacagcgcc
    21781 atgccggcga cgcgcggcac gaccaggtag tcgtagggat cgatgcgcat gcgccgcagg
    21841 ctggcgatct cgcccgagat ctcgcgcagc gcgagctcgg tggccaccgc ggtgccgctc
    21901 ctctggatca cgagcaccgc cgcgatcagc gggccgactt cgcgcagcac cacccagagc
    21961 agcatcttca ccgcgagccc ggcgtcggcg ttcagcacct cgatcacgta cgcgatgatc
    22021 agcgtgccga tgaacgcgcc gcgcagcacg atgccgccgg tcgcgctcgc cccggtgaag
    22081 tagatctggc gcacgagctc ttccgttacg ggcggcaggc gcagcacgcc gagcctgcgg
    22141 acggcatgcc aggccagata cgcgctgcct tcgagctcgc gatagacggc gaacatcccc
    22201 ccggctcccc ttcttgtagt tactacaacg gcttactgaa ggatcttacg ctgaaatagc
    22261 acgcagtaga cgaggacggc ggcaccgagg ccctgcaggg cgccaattcc ggcacttctt
    22321 aacaaatgtc gcagcgcgga gtaatcgaac gcgagcctga agttggccgg ccacgctgtt
    22381 tcggtggacc acagtgcgca aatcagcatc gccccgagca ggccgaagat cacgcaagcg
    22441 cgatggaagc gcgagagtct gggcgcctgc gccgcgatcg gcgcacggcg cagcttgcgc
    22501 cacatccacc aggagagaat cgcgctgcct gcgagcgtgc tcgcatgctg cagccagcga
    22561 tggctatccg aatgcgtgag gtcgtcccac ccgacgtggg tcatggaacc caccaacaat
    22621 gacgcgacca ccgcgtacca cggccgcggc ggcaggccgg cgcacgtgaa gctgccgagt
    22681 cgcggcgaga ggagtgcgat caagggctgc ttgagggcga ggtggaacag tgcatacgcg
    22741 atgaggccgg ccggcacgca gaaccagaag agcgcggcca cgctgtggct gtccgagcgc
    22801 tcgacgagcg gcacgaagta ccacaggtcg ggcagcatgc tgccgatgcc gagcgcggag
    22861 ggcaccgcga accggcccat cggccgcgcg agcggcagca ccgcggcggg atgcgcgaac
    22921 gggtagggca cgccccaatg ctaggggcgg acttgttacg actatgcttc ccggagatag
    22981 ccccaagcgt gcaggcgatc gctgtcctcg taccagcgct cgccgatgtc gtcgcggtag
    23041 tacatgtgcg ggatcatgat gttgcgcacg cggttgtagg cctggcgctg cgcctgccgc
    23101 atggtggggc cggcgccgca cacgatgagc accacgccgg aagtgccggt caccagccat
    23161 tcgccgtcgg cggtgcgcac gtcctcgatg tgcacgccct cgcggctcgg cgtcttgaac
    23221 aggatcaccg agtccttcga ggtgttctcg agggtctcgg ggtcgttgta ggggaacggc
    23281 gggaccacga tgcgcacccc gatctggaag ccgctgcgcg cgcgcaggct ggtggtcgtg
    23341 cccgccgcga gctcgcgcag cagctcgccc atcggcgtga gcaggccctc ctgctggatg
    23401 ctgatcgccg ggtagccgaa gcgcgaggtg aattccagcg ggtagatgcc gttgttgttc
    23461 acgatgcagt tgatgtcgat gtagctcgag tagcgcgcct cggcgagctt cgcctccatc
    23521 ttcttcagcg tggcgttgaa cagcttgttc gggtcgctcc agaacatcga ggtgcccatc
    23581 tcgccggtgg cggggccgat gtcgcccggg aacagcttct tgtgctcgaa attgacgcac
    23641 acgggcgtca cgaactcgcg cccgttgaag aacgcgccga cggccacttc cacgcccatg
    23701 atgcgcttct ggagctggaa ctcggtcatc ttgtccgacc agaagcgctt gtagtcctgc
    23761 agcacctgga tgacgtcgcg cccgtcgtcc tcctcgccca cgaacagcag ccgcttgatg
    23821 ttctgcgcct cgccgctcgg cttgatcacg tagcggttcg ggttcgactg cacgtaggcc
    23881 acggcctcgt cgaacgaggt gaagttctcc tgagagatga tcgacacccc ggccgccttc
    23941 agctcctgct gcccgaaccc gcggtcgtcc tcgaggcggt cggtgtaggt cgagccgccc
    24001 accaccgcct tgccttgccc gcgcagctct tcggccagcg cccccatgcc gagcacgtcg
    24061 tcgaacacca cgacgtccgc ccagtcgacc tcgctcttcc attcgtcggt cttgggcacg
    24121 aagccctccg cgatcccgcg ctcctcggga ttctggatcc agagcttcac ctcgtggccc
    24181 tccttggcca cctgccacgc gatgtcggcg atcagacctt cgtaggaaac gaacaggaac
    24241 ttggtggcca ttcgaggcga agcttactat ccccgctgcc agacgcgcgc gagcacgctc
    24301 gagcgctccg ccggcgtttc gctgcgggca tgcgcacgcc agcacaggtc agtcaggtcc
    24361 agcgcttcgc atccctcggg aatctggctc cctggcgcca cccaggtgcg atgctcgggg
    24421 ccggcgtaga gcttgccgcc gggagcgtcg cgccaatgag cgaaaacggt atcggcggac
    24481 ggtgcgggtt ccgccggcgc gccggcgatc cagcgcggcc cggcggcgaa ccaggcaaag
    24541 gcgaggcagc ccgacgccga gcacgaatcg acgcgaatcc ggttggccgc aatccaggca
    24601 tagccgccgc cgcgccacgt cacgtggttg agccgcagat cgccttccag caccaggaac
    24661 tcttcggcca ccggatagtg gcccgcgccc gggcgcgacc agccggcggg aaagcgcacg
    24721 aacgcgcgga aggcgccgtc ggcgaggtcc ggcagccgcg cgagctgcac cgggaggttc
    24781 gcgcccggca tgggtaccgc cccccattcc agcgaagcga aggcaacgct agccaggcag
    24841 ccgcacctcg gtgctcgctt cggcgaccag cttgccgtcc ttcgtgagtt gctgggaaag
    24901 aacggcgatg tttcccttca cttcggaaac gctccccgag gcgaccagcg tatcgccggc
    24961 gaaggccatc gatttcatct tgaacgagag cctgcggatg aaggcgcgcg gcccggccca
    25021 ggcgagcgcc gcgcgcgcga agaccgcgcc gtacatctgc ccgtcgatca cggcgcccgg
    25081 gaagcccttg ctgcgcgcga gctcgacgtc gtagtgcatg cggtgccagt cccaggtggc
    25141 cgcgccgtag gcgaagagat ccacggacgt gaacgttttt tcgagcggcg gcagcttgtc
    25201 gccggttttc acgcctcatt cacctaaggg ctggacgacg acggtttcgc ggttgatcgc
    25261 cacgacttcg cccttggcgt cgcggtagcg cgcgaccgag gtgacgaaga gctgcgtgcc
    25321 gccgcgcgac gaactgcgct cgacgatgtc ctcgagcgtc caggtgaccg agatgcggtc
    25381 ggccggcagc accgggcgca tgaactcgta gtcgttgccg gcgcggatca ggcggcagtt
    25441 gcgcaccggc aggtgccatt cgtggccgat gtatccgtcg gcgttgggcg cctggtgcgc
    25501 gtactgcgag gtctcgcaga tcagcgtcgg cggcgcgatc accgagggat agcccgcctt
    25561 cttggcatac tcgtcgtcga cgtagagcgg gttgtcgtcg cccatcgcga gcgcgaaata
    25621 gcggatcgac gcccggccga gctcctcgcg ggcggcgtag tgcgcctcgc gcccgatcca
    25681 ggctttcagc tcgtcagtga ggagcgccat tgcagtccac gcatctagtc caatatcgcg
    25741 aacgggtcta cctcgatctg catctcgggg cgcagcaacg cctcgcagac caggccggtg
    25801 gaggcgggcc agggcttgtg cagcagcttt tcgcgcactt tcgccacgtc ccggtagcgc
    25861 gcgagcgcgg cgggcgtcac gtattcgatg gtctttacca ggttgcgcgc atcgccgccg
    25921 gcagcttcca gcaccttgat cacgttgccg taggtgtact cggcctgcgc cacgacgtcg
    25981 ccgtcgtgca ccacgcgctc ggtcgcggga tcgagcgagc cctggccgga catgaacagc
    26041 agcttcccgg cgcgcaccgc cgggctgtag gtgagcttct ggtagcgcgt ccagccggga
    26101 ttgaccgcca ccggcgtgtc gcgcgtggcg atgaagtcgt actggatcag cgcctccggg
    26161 tgaacgagcc gcggcatcag gatgccggcc gcgcccggat agaccgggcc gaggtggtcc
    26221 ctgcgtaccc ggccggtccc cttgtaagcg gcgagcgcag ccggggtgat gtagtcgagc
    26281 gtcttgacca cgcggtcgaa gccgacgccg agtgccgcga gcatcctgct ggcgcgctcg
    26341 aagatggcgc gggtctgcgc ggccacgtcg cccgcgccga cgatgttgcc ctggtcgtcg
    26401 accggctgga cggtgggcag gaacacgact ccggaggttt ccttccggcc cggtaccgcg
    26461 ctcgcggtaa cctcgatctc gatgaacgcc tcgggccgca gcagcgcctt gaccggcacc
    26521 gtgttcacca ccggctgatg tcccttgaac acctcgcggc gcacgccatc ggcttcggca
    26581 tagcgctcga tgccttccgg cttcacgtac tcgacgaccc gcaccacgtc ggcgtagccc
    26641 aggccgccgg actcgaggat cgcgccgatc ttggcccacg cggtgcgcac ctggtcggtc
    26701 atcgtgccgc gcaccacgat gcgcttcgcc gccgcgtcgt attccgagcc cgtatggccg
    26761 gaaaggtacg tcccggtggc gctcctcacg ccgagcgaga acgaatagcg cgagtagtcg
    26821 aaccagggaa atttcgccgg cttgaggtcg gtcagggtca tttggtgaac atcggaatca
    26881 tctcgccgcc ttccgccgcc ttgaacacga ggcgcacggg atcgccgatc cgcaggcggt
    26941 ccagctcgca gtcgaccatg ttggtgagca tcgtcacgcc ttcgtcgagg gtcacatagg
    27001 cgatggcata gggctcggcg acgcggcgca tcacgctgta ggagtagatc ttgccagtgc
    27061 ctttcgcagc aacgagctct gttttgcccg agcagaacgg gcagacggca cgcgggtagt
    27121 agtgcagctc ctggcaagcg gagcattttc caagaagcag cttgccttcg cgcgcggcat
    27181 cgaagaaagg cctgttctcg ggattgaccg gcggcgaggg aatcttcatg cgcgctccag
    27241 gatgagcgtg gcgctgccgt ggcgcgagcc gagctggccg ccggtgccgt tcgccagggc
    27301 gaggcggcag ttcttcacct gcaccgcagg gtgcgcctcg ctgcggagct ggcgcaccgc
    27361 ctcgatgacc ttggtcatgc cgccgcgatt tcccggatgg ttgttggaga gcccgccgcc
    27421 gtcggtgttg aagggcaggc ggccgctgcc cgagaccagc ccgccgtcgg acacgaagcg
    27481 tccgcccgcg cccttggcgc agaagccgag gtcctcgagc tgcatcagca ccgtgatggt
    27541 gaagctgtcg tagatcgaaa cgtactggat gtcggcaggc ttgatgccgg cctcgtcgaa
    27601 cgcggccgcg ccggaccacg cggacgccga ccaggtgagg tcgatcctgc cgccggcctg
    27661 gcctttcatc gcttcgccgg cgccgatcac ccgcaccacg ggacgcttga gatcccgcgc
    27721 gatctcgggc cgcgtcacca ccagcgcgcc gccgccgtcg ctcaccacgc agcagtcgag
    27781 ccggtgcagc gggtcggcga tcatcggcga gtcgagcacg tccttcaccg tcaccacctc
    27841 gcgcagcatc gcgttcgggt tgtgctgcgc gtggtgcgag gcggcgacct tgatccacgc
    27901 cagctgctcg ccggtcgtgc cgaactcgtg catgtggcgc atggccacca tggcgtacat
    27961 gttgaccgtg gcggggccgt agggaacctc gaatgcggcg tcgggcgccg cggtgccgta
    28021 gttacgcggc tgcgtgccgc tcgagccctc cgagcgcggc cgcccggcga gagtgatcag
    28081 cgcgacgttg cagcgacccg tggcgatggc ttgcgccgcg tgtgcgacgt gcacgacgta
    28141 ggaggacccg ccggtctccg tggtgtccat gtggcgcacc ttgagaccga ggtaatcgag
    28201 catcgacagc gcgcccaggc ccggcgcgtc gccggcgcag aagtagccgt cgacgtcctt
    28261 gcggtcgagc cccgcgtcgg ccagcgcgcc tttcgccacc tctgcgtgca gctgtgcggt
    28321 cgatttgtca ggcgccttgc gcgtcggatg ctcgaaggcg ccggcgatgc aggcttttcc
    28381 cttgatcgtc attggatcgc cttcgccttc gaccgggcgt tgtcctcgac cttgatcgca
    28441 tccgccgaga gatgcgcaaa aacgtcctgc ggcgcggcga tggtgcggat tttcttctcc
    28501 aggcccttga tgcgctcgcg ccaagggtag ctcagcgccg cttcccacgc ggggccgtag
    28561 ctcgccggct gcttcatcgc ttcgcgcacg cggatcgtca gcgcctgcgg atcgatgcga
    28621 tggccgccct cgagcacgtt ctcgtgcctg cgctcgaacc agggccacca cagctcggaa
    28681 tcgcgcacgt ggttccaggc gcgcagccag tggctgccgt cccagaccgg ctcgaccgca
    28741 ggcgcatacc gcgacgcgat ggactggtct ttcaggaaag cgggcgcgtc gagcacgacg
    28801 tccgccccac gcctgtgcgc gagctcgagc gcgacggcgg cggcgccgtt gtgggcatag
    28861 acgcgcaggt cgccgagctt catcttttca agaagattga gtacggcaag cgcaaggctg
    28921 cgtggatcct gaggattgcc gggaagggga tccgactcgc cgtggccggg caggtcgacg
    28981 gcgatggcgt tcccggcttc gagaaccaga tcgtcgtaga gcgcggatga gccgggggaa
    29041 tggtgcagga tgagcagggg ggatttttca agtttttcaa aacgaacaaa aagttgcgtg
    29101 ccgccgatat cgacgtagtc cgtcgtggtc cgcccggcga gcggcgcgca gcgcggggcg
    29161 ggcgggggtg cgccgcgcgc cgggtgcttg agcaggatgg cgcgctcggc gagcgtcgcc
    29221 ggcccgaatt cgcgcggcat gacctcctgc caggcggaag ccgggtagag cggccgcgta
    29281 agaaacatgc tgtcgccggg gcggttaccg aagcacaccg gcactttcag gtcggcgagg
    29341 acgtcgagcg cccggtgtcg gaaaggcgcg gcatagccga tgcggtagtc gttgccggct
    29401 tcgagcatct cgacgacgcc gcggtgcagg aagtcggcgc cggggatgtc cgcgtccgag
    29461 cgtcccgccg ccgtctggtt gttccacggc cagaaggcgt gctggtcgcg gtagcgcgcc
    29521 cagaggcgaa gcaggtggcc gccgtcccac gccggaacga tcggctccag ataggcctcg
    29581 agctgctcgt cggtgtagcg cttctcgaac acggcatagc cgtcggcgag cgccatcgcg
    29641 gtgcgcttgg ggtggcgtgc ggcgaactcg actgcgatcg aggcgccggt gtggcgcccg
    29701 taggtggcta cgtgctcgac gccgagcgca tcgagcgtct ccgcgagcgc gtcggcgaag
    29761 tcctcgatgc tcggattggg tttcggcagc aggtccgaaa gtccgaaccc cggcatgtcg
    29821 aaagcgaggc aggtgaactt ctcgccgaac accggcatca gcgggcgcat gaccttcgcc
    29881 gagcaagggc aggcgtgcag cagcgcgagg gcgggcccgc tgccggcgcg caggtaatgc
    29941 acgcgccgcg atcccaccgt tacgaaatgc ctcgtaatcc gcatctagcg cgtgaatcga
    30001 atcgtctcaa taggtgcgcg gaagtcccag tcgctcgccg acgaagttgc gcaccatctc
    30061 gttcgagatc gggctgaacg ggccgaggcg ggcgtcgcgg aacaagcgct ccagcggcgt
    30121 ttcctcgacc aggccgaagc cgccgagcac gcgcatgcac cggtcggtcg cgcggatcgc
    30181 cgcctcggtg gcggcgagct tggccatcgc tgcttccgta gcgcattcgc gcccgttgct
    30241 ctgcagccag gcagccttgg cggtgaggag ccggatctgc tcgagctcgg tcgcggtctc
    30301 ggcgagcggg tgctgcaccg cctggaaagc gccgatcggc cggccgaacg cctggcgatc
    30361 gcgcgcgtac tgcagcgcct ggtcgagcgc ggcgctggtg atgccgacgt acatcgcggc
    30421 gcaaagaatg cgctcctcgt cgagcgaggc gaggagcgtg cgccagccgt tgccttgccc
    30481 gccgaccagc gcgtcggcgg gggcgcgcgc atcgtcgagg aacacctcgc aggtgcacgc
    30541 ggcgcgcatg cccatcagct ccaggcgccg cacctggacc gcaggctggt ggcgcggcgt
    30601 aagcaccagc gacaggccgc gcgcgcgctt tgcgccctcg gccgggtcgg tgcggcaaag
    30661 caccaggatc gcgtcggcgt cgtcggcgaa cgaggtgtag agcttctggc cgcgcacgac
    30721 ccactcgttg ccctgcagcg tggcgcgcgt cttgagcgcc agcacgtcgg tgccgccgcc
    30781 gggctcgctc atgccgaagc tcatcaggaa ctcgcccgcg gcgaggcgcg gcagcagcgc
    30841 cttcttctgc gccggggtgc ccgactcgcg cagcacgcgc gcggtcatcg acaccagcag
    30901 ccaatcggtg gcgagcgagg ggaagcggcg cgccagctcc tcgcagaaga cagcggcggt
    30961 cgagacgtcc gcgccgctgc cgccgtactc agtcggcacc gagagtcctt gccagcccat
    31021 ggcggcgaag cgtttccaca gctcccgcgg aatgcggcgc gcgcgatcca tctcgcggat
    31081 gcgcgcctcg ggcagctcgc gatcgaggaa ctcgcgcacg ctgtcgcgca ggatcgagtg
    31141 ctcggatgcc ggctcgaagt tcatgccccc gctcccagat agacctgctg cacttcgggg
    31201 tgcgcccgca cctcggcggg cgagccctcg gcgagcacgc ggccctggtg catgaccgtg
    31261 atccgcgtgg cgacggcgaa gacgaccgcc atgtcgtgct cggtgaagac gcacgacagg
    31321 ccgagctcgc gcgccagcct gtgcaccagg gtgatcgact cgatgcgctc ctgcgccgcc
    31381 atgccggcgg tcggctcgtc gagcagcagc aggcgaggct cgccggccag cgcgatggcg
    31441 agctcgagcc gcttctggtc gccgtgcgac agggttccag cctgtctttc aaaagcattg
    31501 gccaaaccta cgagcgcgag cagctccttc gcccgtgcgg catggagcgc cgccgccttc
    31561 ggccagacgc tccacgcgcg ccgcgcgtgg gccagcagcg acacctgcac gttctcctgc
    31621 gccgtcagat cgcggaagat gctcgtgatc tggaaggtgc gcccgattcc catgcgcgcg
    31681 agcaggtgcg gcgcgctgcc ggtcacgtcg cgcccttcga agctgacgcg tccgtcgtcg
    31741 ggccgcagca ggccggtgag cgcgttgaag aaggtgctct tgccggcgcc attcggaccg
    31801 atgatcgcgc ggaactcgcc cgcctccacc gccaggtcga cgccgtcgag cgcgcgcagg
    31861 ccgccgaagc gcttggcgag tccctggacc tgcaggatca cgccggtctc cttcgtgcca
    31921 ggcccgtcag tccttgcggc atgagaagaa ccaggaccag gatgatgatt cccatggcga
    31981 gcggccagta ctcggtcttg ccgctgatca gcacttcgag gaccacgtac accgcggcgc
    32041 cgagcgcggg gccgaggaag gtgtgcacgc cgccgagcac ggccatcacg atcggcgtgg
    32101 cgctcgccgt ccagttgagc cagccgggga acacgttgcc gttcgagagc gcgaacaggc
    32161 cgccggcgag cccggcgaag gcgccggtga tgatgaaggc ggcgagctgg tgcgtgcgca
    32221 cgtcgacgcc gatgtactgc acgcggcgcg cattcgtgcg cagcgcgcgc agcgtgtagc
    32281 cgaagggcga gttcaccagg cggtaaagga cgtagagcgc gggcgccacc agcgcgagcg
    32341 cgaagtagta gtaggcgagc ggcttcgcca gcacgccggg cgggcgcagg ccggtcatgc
    32401 cgtcgtcgcc gccggtgagg ccggtcgcct tcagcgccac cgtgtagagc agcatctgga
    32461 acgcgaaggt gagcatcgcg aaatagatcc ccgagcggcg caccgagaag aagccggtga
    32521 ctgcggcgaa tgccgcggag gcagccatcg cgaccagcgg caacagcgcg aaggcggcgg
    32581 cggcggaaag cccggtcttc ttcatcagca gcgccgcggc gtagcctccg acgccgaaga
    32641 aggcagcgtg gccgaacgac agcatgccgc catagccgag cagcacgttg aagctcaccg
    32701 cgtagagcgc gaacgccagc acctcgatgg cgatgaacag ccagaagtcc gacagggcga
    32761 gcggcagcgc gaggagggcg atgccgcccg cgagcggcca gcgcgcggcg ccgctcattc
    32821 gggcgcgccg aacaggcccc agggacgcac gatgagcacg atcgccatca cggcgaaggt
    32881 gagcacgacc gcgaggccgg gaaaggcgag cacgccgaag gcgttcacga tgcccaccag
    32941 cagcgaggcg acgagcgcgc cgaggacgct gcccatgccg ccgatcacca ccacgacgaa
    33001 ggcgtcgatg atgacctgca cgtgcaggcc ggggccgacc gccacgatcg gcgcggcgag
    33061 cgcgccgccg aggccggcga gcgccgcgcc ggccgcgaac acgctggtga acaggcgccg
    33121 ggtgttgacg cccagggcgc tcagcatgtc gcggtccatg gtcgccgcgc gcacgaagat
    33181 gccccagcgc gtgcggtgca ggacgatcca caggccgacc gccaccgcca cgccgacgac
    33241 gatcaggaac aggcggtagt tcgggaaggt gaggccgagc gcgcgcatgc tgccgtcgag
    33301 cgcggcgggc accgcgaccg agtggtcctc gcgcccccag atcagcttcg cgacgtcgcc
    33361 gaggacgaag accagggcga aggtgagcag cagctggaac tcgtgcgggg cgcggtagat
    33421 gcgccgcagg agccccgcct ccagcgccgc gccgagcacg gcgaggccgg cggttgcgcc
    33481 gatcagcgcg gcgacgaagc ttgcgctggc gccgggcgcg agcgcgatga ggctgaccgc
    33541 aatcagcgcg ccgagcatga acagcgcgcc gtgcgcgaaa ttgatgatgc gcagcacgcc
    33601 gaagatcagc gacagcccgg cgcagatgag gaacagcagc atgccgttcg acacgccggc
    33661 gaggagctgg cgcgcgacga cgaccgggtt ttgcagccag gcaagctgct gcaggaagga
    33721 gtcgatcacg ccgatcgagt atgccggaag cgccttgaca acgggagagc cgtgtccgga
    33781 caattcgcca caacccgcag tacaacccta ctcgaggcga ccatgctacg aacccttctc
    33841 atcctggcgc tgtgcgttgc gccgccagcc gctcaggccc agtcgcgcga gccggtcaag
    33901 ctggggctga tcgacatcta cagcggcggc ttcgcgttca tcgcggacag catccgcacc
    33961 ggcttccaga tcgcggtgga cgaggccaat gccgccggcg ggctgaacgg ccgcccgttc
    34021 cagctcgtca ccgccgacat gggcaactcg gtcgagaaag cggtcaccga gggccggcgc
    34081 atgatcctcg aggagaagat caagttcgtc accgtcggca tccacagcgg cgcggcggtg
    34141 gcggtgggca acctcggcaa ggagcacaag gtcctgatca acggcggctt cgcgaccacc
    34201 aagcgcctca ccggcgaagc cggccacgac tacgtcgcgc gcgccaacct ctccacggtc
    34261 gagatcggcc gcgtgatggc cgagcacctg aagtcgcgct ccgacatcaa gcgcgtctcg
    34321 gtgatctcgc ccgactacga gtatggccag cacttcgcgg cggatttcat cgccgggctg
    34381 aagacggcgc gaccggacat cgccatcgtg cgccaggagt ggccgaagct cggcgccacg
    34441 gatttcgggc cgcacgtcac cgcgctgcag tcgcagccca ccgacctggt ggtggccggc
    34501 atgttcggcg cggacctggt caacttcctg cgctcggcga aggacttcgg cctgttcagc
    34561 gacaagacgc agttcatggt gcacggcctc gacctcgcca agatgggcgc gctcaaggat
    34621 tcgctgccgc ccaacaccat gggcacggtg tggtacccgt tctatgccat cgacagcgcg
    34681 aaatccaagg cctttgccgc cgaggtggaa aagcgcatga agacctatgc tacgggttcg
    34741 accacggtcg gctacgtggc cggcaagatg ctcaccgagg cgatccgcaa ggccggcacc
    34801 gacgacgtcg agaaggcgat caaggcgctc ggcacggtgc aattcgaggg tcccaccggg
    34861 ccgaccaaag tgcggagctg cgacaacatg gcgctgtaca acttctatgt cggcaccgtc
    34921 aagcgcgacg ccgcgttgcc cgacggcatc ggcctgggcg acgtcaaggc ggtcaatacc
    34981 gagaccgtgg cgcgcagctg cgccgacgtg aaaaaggcgc gcagcggcgg ctagccgccg
    35041 gtggcgctgc tcgaagtccg cgggctggag accgcctacg gcgcgagcca ggtcctcttc
    35101 gggatttcgc tcgaggtaga gcgcggcaag gtcgtcgcgc tcctcgggcg caacggcgcg
    35161 ggcaagacca ccacgctcgc gagcgtgatg ggcctggtgc ggccgcgcgc cggctccatc
    35221 cgcttcgacg gcgccgagat cctggggcgc gagccttttg aggcctgccg cgcaggcctc
    35281 ggcttcgttc ctgagaactg ccgccttttc gccgggctca cggtcgccga gaacttcgag
    35341 gcggcgcgcc gccccacgca agcgggcgaa gggcgctggg acctggacga ggtcctggat
    35401 ctctttcccg acgtgcgaag cttcctcggt cagcgtgcgg gcctcctttc cggcggccag
    35461 cagcgcatgg tcgcgatcgc gcgcacgctg ctcggcaacc cgaagctgct gctgctggac
    35521 gagccgtccg aaggcctcgc gccgctggtg gtcgacgcca tcctgcagcg gctcaagcgc
    35581 ctgaaggcga gcggcaccac ggtgctgatc tccgagcaga acctgcgctt cgccaccgag
    35641 ctcgccgacc gcgtgtcgat catcgagcgc ggcgagatcc gctacgaggg cacgcccgcc
    35701 gagctcgccg ctcgtcccga ggtgcgcgaa aagtacctga tggtctagcc gggcagcgtc
    35761 gggtagtcgt tgtagccgtc cggcccgccg ccatagaagc gcttggtgat cgccttggcg
    35821 tagggcccgc cgcggcggat gcgctcggga aggtcgggat tggccatcca tagccggccg
    35881 aaggaaacgg cgtcgaccgc gccggcgccg atgagctggt tggcggtgtc gggatcgatg
    35941 ccgccgttgg cgatgatctt gccgtcgaat accggccgca ccagctcgag catgcgcgtg
    36001 cgctcgaact tgccccacca gaagctggtg tcggcgagct ccagccatcc caggccgctt
    36061 tgattcaact ctttcaccag gaacctgaaa gcgggggaag ggtccggatc gaccgcgccg
    36121 ttgtagtcgg tgtaggggga gatgcgcatc ccgacgcggg cgggcggata gtgcttcatc
    36181 atcgcctgca ccacctcgat cgcgaagcgc gcgcgcttct ccggcgtgcc gccgtagtcg
    36241 tcggtgcgcc ggttgacgaa cggcgagagg aactggtgcg gcaggtagcc gttggcgccg
    36301 tgcagctcga tggcgtcgaa gccgatctcg cggcaggcgc gcgcggcctc gccgaaggcg
    36361 gcgacggtgc cgcgaatctc ctcgagcgtc atcgcgcgcg agggcgtcgc cttgagcttg
    36421 atgaacttgc cgtcgtcgcc gcgcccccag accgtgatcg cctcgaggtt ctcgttcacg
    36481 cccgagggcg acagggcggg gaccggcttc ggcgagcggt cggcggcgac gcggccgccg
    36541 tgccagagct gcaggacgat cctgccgccc gccgcatgca ccgcggcgac cagcggccgc
    36601 caggcgtcga tctgcgccgg cgtgaagatg ccgggcgcgc attcccaggc gttcgactgc
    36661 gcgacgacgt tggtcgcctc ggcgatgatc aggccggccg aagcgcgctg cacgtagtgc
    36721 tcgatcatca tcggcgtcgg cacgcgctcg ggcgtggcgc gcgagcgggt gagcggcgcc
    36781 atgacgatgc ggttcggcag cgccaggtcg ccgatggcga gcggcgagaa cagatcgctc
    36841 accaggccat cactccagct ccccgggaag ccccgccatg agctggtcct tcagcttgcc
    36901 gcgctcgacc ttgccggtgg gattggacgg gatggcgtcg atgaagaagt agtggcgggg
    36961 gcgcttgaag tcggcgagct cggggctctt caggcagaac tcgtcgagcg ccgccgccga
    37021 gagcgcggga tccttgcgca cgaccgccgc gcacaccacc tggccccact tgcggctcgg
    37081 caggccgacc gcggccgcgt tggcgatgcc gggatgcttg aacagcacgt tctcgatctc
    37141 cgagggatgg atgttctcgc cgccgctctt gatggtgtga tccatgcgcc ccatcacggt
    37201 aatgaagccc tcgtcgtcga gcaccgcggc gtcgcccgag aggaaccagc ctttcgcgaa
    37261 tgccgcggcg gtgcgctcgg gcaggccgaa atattcggtg agcaggctcg ggccgcgcgt
    37321 gatgagctgc ccgatctcgc cgggcttgca caggtcatcg ggctcgcggc tgtcggtgcg
    37381 gatcacccgc accgccatgt tgaccgaagc cttgcccacg ctgccgagct tgccgggata
    37441 cttgcggtag tcgcagcagg tcaccatgag cgcctcggtg gagccgtaga cgttcgcgaa
    37501 gtactccggg aaccaggcca tgaccttgcg cgtgacgtcg tcggcgaggg tctggccgcc
    37561 gaagaacccg cggcgcagcg agcgcaggtc gtacttcgtc aggtcggcgc cggccatcat
    37621 caccatctgc gtcggcacgc cgtgcagcac ggtgatgcgc tcggctccga cctgcgaaag
    37681 cgtctcgtgc acgtcgaagc cgcgcgtcag cacgttggtg ccgccgaggt gcacgaacgg
    37741 gatgaactgc agctgcaact gcgcgatgtg gaacagcggc ttgttgttca ggatggtgtc
    37801 gtcggcgcgg aagccgcact cgacctgcat cgagtggctg cccatgatct cggcgaggtg
    37861 cgagcgcacc acgcccttcg gccgcccggt ggtgccggag gtgtacatga tgaaggccgg
    37921 gtcgtgccag cccacttccg cctcgggctc ggcctcggag cccggcgcga tgaacttctc
    37981 gaacgggatc tccttgcctt ccggttttgc tcccacataa atcaattgac tgacggaaag
    38041 gcgatcctgg atggccgcca ccggcgcgcg cagcgcttcc tcgaacacca ggatgcgcgc
    38101 tcttgagtcg tcgacgatgt acttcacctc gttggcggcg aggcgatagt tgatcggcac
    38161 cggcaccgcg ccgatcttca tcagcgcgta gaacccggtg acctgctcgg gcaggttgta
    38221 ggtgaggaag gcgacgcgct cgcccttgcc gatgccggcc gcgcggaagg cgtgcgccgc
    38281 cttgtttacg ctgcggttcc actgcgcgta ggtccagcgc cggcattcga acaccagcgc
    38341 ggtcttgtcc ggccagttgg ccgccgactg ggtgagcgtg tgcgcgaggt tcatcccaca
    38401 aatttatccc gagatgacac ccgcagcgcg cagcgtcgag atgcgctcgt cggaataccc
    38461 cgcggcacgg agctgctcca cggtatcggc gcccatcggg ggcgctgcgc gggtcggcaa
    38521 ctcgacgccg gggaaccgga tcggcgccgc gaccatgcgg gcgctgccgc cggccgggta
    38581 ggcgaactcc gctacgccgc cgcgctcggc gacgaactcg ctctccagcg cctgcttgac
    38641 gtcgtacacc ggcgaagccg gcacgtttcc ggcgaagcgc tcgatccatt ccgcggtcgt
    38701 gcgcgtcatc agcgcggcgt cgagcatctg cgtgacgcgc tcgcggttgg cgagccgcgc
    38761 cttgaaggtg gcgaagtcgg gatccgtcgc ccattccggc ttgccgagcg cctgcgccag
    38821 cacaccccag aacttttcct tgttgcacat gatgaagatc cacccgtccc gcgtgcggta
    38881 gagctggctc ggcgtcaggc tgggatgccc cgagcgcggc gtgcgcccgg tgacctcgcc
    38941 ggcattcagg aaccacgtcc ccgggtagtt gaggttgtgc agggcgacgt cgaagagcga
    39001 ggtgtcgacg tccatgccct tgccggtggc gcgtgcgctg agcacgcctg acagcagccc
    39061 gaatgccgcg gcgagcccgg tcatgagatc gacgatcgac aggccgtagc gggtcggcgg
    39121 gccgccgggc tccccggtga gcgacatgtg gccggcttcc gcctgcatca ggtagtcgta
    39181 gcccggccaa gccttgcgcg agccggtccg cccgtaggcc gacaggtgcg cgcacacgat
    39241 gcgcgggttg acctgcttca ggtcctcgta ggtcagcccg agtttctccg ggtggtcgcc
    39301 gcgcaggttg tcgagcaccg cgtctgctgt cttcaccagc gcgcggaagg cggccttgcc
    39361 ttccggatgc ttgaggtcga gcgtgacgct cttcttgttg cggttgaagg tctggaagaa
    39421 ctggctgtcg cccgggccga agaaatgcgg cccgacgtgg cgcccgacat cgccgccatc
    39481 cttgtggttc tcgatcttga tcacttctgc cccgaggtcg gcgaggtaca gcgtgccggc
    39541 cgggccggcg ccatactgct cgaccgcgag gatgcgcagc cccgcgagcg gcggcgtcac
    39601 gtaactgcct ccagggcgaa gtccgcggcg agcagggcct cgagctccgc ggcgtcctcg
    39661 atcgactcga ggtccagcac ggcgttgcga atccgcttcg cggcactttc ctcgagcgtg
    39721 agcgcgcagt tctcgaagta cttcgcctcg atctccgcgt tggtgagcgg gcggtccgga
    39781 ttgccgcggt tcaccgcgac
//
LOCUS       DATBSZ010000200        15357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2426204, whole genome shotgun sequence.
ACCESSION   DATBSZ010000200 DATBSZ010000000
VERSION     DATBSZ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15357
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2426204"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VIG70_12905"
     CDS             complement(<1..575)
                     /locus_tag="VIG70_12905"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY8251497.1"
                     /translation="MLCLTKSQIEEFYDAGYVVLPGVFRDAEIREIGKAFDRLRDTAI
                     KLTRPKMIKGSYFVVNDHRINRIVWCGAAEPVLLKYGEDARLTFPSSQLLGINEMEQL
                     ICQAHFKLPADGVGFGWHQDSDHRRYGTDQWTDVNGRGSFVQTLMAVDVMNEENGPLM
                     VVPGSGRQGHQYLKQNPSPLDALFDGRAQPV"
     gene            complement(618..1727)
                     /gene="dnaJ"
                     /locus_tag="VIG70_12910"
     CDS             complement(618..1727)
                     /gene="dnaJ"
                     /locus_tag="VIG70_12910"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY8251498.1"
                     /translation="MAKRDYYEVLGVNKDASDDDLKKSYRKLAMKWHPDRNPDNPKAE
                     EHFKEAKEAYEILADANKRAAYDQFGHAGVTPQAGGGPGAGFGDIFSDIFSDIFGGAR
                     AGRSTVFRGADLRYNLELALEQAAHGFETKIRIPSAAVCETCKGSGARPGTQPASCPS
                     CRGAGQVRVSQGPFSIAQTCPRCHGSGSVVPNPCGPCAGTGRIKLHKTLAVKIPAGVD
                     EGDRVRLSGEGEPGVNGGPPGDLYVQVHIKPHSVFQRDHDDLHCEMPISITTAALGGE
                     ISIPTLDGSAKIRIPAETQSGKTFRLKSKGIKGVRSQFPGDLFCHVVVETPVSLTERQ
                     RQLLREFEGISAQDAARHNPRAKGWFDKVKEFFDG"
     gene            complement(1730..3688)
                     /gene="dnaK"
                     /locus_tag="VIG70_12915"
     CDS             complement(1730..3688)
                     /gene="dnaK"
                     /locus_tag="VIG70_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY8251499.1"
                     /translation="MAKIIGIDLGTTNSCVAIMEGGKPKVIENSEGARTTPSVVAFTD
                     DGEVLVGASAKRQAVTNAKNTIYAVKRLIGRKFDEKETQKDIKLMPYKIARADNGDAW
                     IEVRGKKISPQEVSAHILRKMKKTAEDYLGEEVKDAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAGALAFGEDKIAKKDQKIAVYDLGGGTFDISIIEIADVEGEKQFE
                     VLSTNGDTFLGGEDFDQRLIDYIVSEFKKDQGVDLSKDVLALQRLKESAEKAKIELSS
                     SQQTEINLPYITADQSGPRHLNMKITRAKFESLVDDLIEKTIAPCGIAIKDAGISAKD
                     IQDVILVGGQTRMPKVIDKVREFFGKEPRKDVNPDEAVAIGAAIQAGVLKGDVKDVLL
                     LDVTPLSLGIETLGGVMTKLIQKNTTIPTKAQQVFSTADDNQNAVTIHVLQGERDMAG
                     GNKSLGQFNLTDIPPAPRGMPQIEVTFDIDANGILHVTAKDKATGKENKIKIQASSGL
                     TEAEIQRMVKDAEAHAEEDRKTLELVNARNQAEALVHSVRKSLKDYGDKLDAAEKEKI
                     EAAIKDAEGVLKSEDKAEIDAKSQALAQAAQTLGQKMYADQQKAAAEAAAGSAAGSAQ
                     GQAEQPGKKDENVVDAEFTEVKDKKTGS"
     gene            complement(3717..4232)
                     /gene="grpE"
                     /locus_tag="VIG70_12920"
     CDS             complement(3717..4232)
                     /gene="grpE"
                     /locus_tag="VIG70_12920"
                     /inference="COORDINATES: protein motif:HMM:NF010737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY8251500.1"
                     /translation="MQEETVQPQNEKSLEELLAEAHARIEQQRDTMMRAVADAENGRK
                     RAQAEAATAQKYALERFAEGLLPVMDSLEAALKTRDVSGVELTLKLLRSALEKARIRE
                     IDPKPSERFDPHRHQAMAAVEADADPNTIVSVMQKGYILHDRVLRPALVTVAKALEKS
                     DGNPISDTGLD"
     gene            4310..6055
                     /locus_tag="VIG70_12925"
     CDS             4310..6055
                     /locus_tag="VIG70_12925"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY8251501.1"
                     /translation="MNKPLKYGLLGLGTLVVVAVAGAVVFAMTFDPNRYKGEIERLAK
                     EKTGRTLAIKGDIKMAFWPSLGAGVAGVSLTEKGSNQAFVSFDNVHASVKLMPLLSGQ
                     YIVDSVSLAGLKARIVKQKDGRFNFSDLMEADAKKPAEPKKSPEEKTGPVVFDIGSIS
                     IERSSIAYLDLQAGQEYALEDVKLKTGRIAQDAEGKLELAMVAKRKSPPLQAKLSADG
                     KYQLKGGVLSGDVTAKLDDSTIKAKFTAAEPYEFDAAIDKINLDRYLGAAEKPAAQPQ
                     KQETAKSKQEDTPVDLSALKGINAKGKLQVGALEVRGLKLADVSAQLNAANGKVTVAP
                     HSAKLYEGTISGEVAVDANKNQVALKEQLQNVSVGPILRDFAEQDRLEGKGNVALDVT
                     TSGATVNAMKRALNGTAKVQLRDGAIKGINLGEILRKARTALGGSQSAADGQSAGDKT
                     QRTDFSELNASFAIKNGVAHNEDLDVRAPLFRLGGAGDINIADSSLNYLAKASLVATS
                     QGQGGRDRDTLAGLTVPVRLTGTFDELKYEVDYRGLAGAAAKSQVGGKIKEQLEERVL
                     KDEKLKERLKGLLGR"
     gene            6129..8198
                     /locus_tag="VIG70_12930"
     CDS             6129..8198
                     /locus_tag="VIG70_12930"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8251502.1"
                     /translation="MLNLLRYSQLIALAVLCGVVVALSLLYRGLLFDSMIESETHASV
                     ALTKTFANAVWPSHAAFVGRAGSLDRATLAGRPEIAALDHDLRRLSEALPVVKVKIYD
                     LHGLTVYSTDASQIGQDKAANPGFKRARDGYAASNLTFRERIDSWEGGELADRHILAT
                     YVPVHVYDAAPVEAVLEVYTDVTELVARMERGQWQIIAVVLGAMALIYLLLQLILARY
                     QRLLAEKERERAAQEERIRHQAYHDALTGLPNRASFTEHLEEAMRRAKRAGWPLAILF
                     LDLDLFKRVNDSLGHDAGDRLLRVAAERIRRAVREADMLFRMGGDEFTVLLEDVRGPE
                     EAAAVAGRVLEGIAEPLQLQHHELAVTASIGIALYPRDDVLGERLVKSADTAMYRAKE
                     LGRNRFAFFAKEMNERVESQIMIETALRRALKNDEFVLHFQPRVSAATGRATGAEALL
                     RWKHPEWGLVEPARFVPLLEETGLIVPVGNWVLLEACRQAMAWQAAGLPPLRVSVNLS
                     SRQFRSEALFEAVSEALRASKLAPQLLDLELTESLLIDNVDHAMGVMGKLKAIGTAIS
                     IDDFGTGYSSLGYLKRFPIDSLKIDKSFVRDIATSPKDAAIVNAISALARSLGIGLVA
                     EGVEEPWQVEFLRARHCTEMQGYLFSRALPAESLAAALSRSYPMPRPSAASASIQSGD
                     SLRQFTR"
     gene            complement(8123..9010)
                     /gene="hemH"
                     /locus_tag="VIG70_12935"
     CDS             complement(8123..9010)
                     /gene="hemH"
                     /locus_tag="VIG70_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY8251503.1"
                     /translation="MKSVLLVNLGTPAAPTPEAVKSYLAEFLGDPRVVKLPRWLWLPI
                     LHGIVLRTRPKRSAEKYSQIWTSEGSPLAVHTVRQAALLQEKLGIPVRYAMRYGQPGI
                     RTSLENLAEPLVIPLYPQYAESTTASIADMLPRGVPMLESFHDHPAYIAALAANVRRH
                     WQQHGRGARLVMSYHGLPKKGSERYEKECRATARLLAERLKTEYLVTFQSRFGYAEWL
                     QPYTEPTLTDLARKGTGRVDVLCPGFVSDCLETLEEIGIVARRAFLAAGGREFYLVNC
                     LNESPDWIDALAALGRGIG"
     gene            complement(9007..10020)
                     /gene="hrcA"
                     /locus_tag="VIG70_12940"
     CDS             complement(9007..10020)
                     /gene="hrcA"
                     /locus_tag="VIG70_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY8251504.1"
                     /translation="MLDKRAQILLKTLIERYIAEGQPIGSRTLSKYSGLDLSPATIRN
                     VMADLEELGFIASPHTSAGRVPTPLGYRFFVDTLLVIKPLESNALLRIEGELHPDNPQ
                     RVIQAASQLLSQLTQFAGVVMTPRRAVTFKHIEFLRLSEKRILMIVVTPDGDVQNRIL
                     FTEVEYRPAELIEAANYINQNYAGQSFEDVRARLQGEVREVREDMTRLMSAALDAGSA
                     LAEGGEQYVISGERNLFAVRDLSQDMGRLKQLLELFERKTSLIKILDLALRGQGVQIF
                     IGGESGVVAPDDVSIVTSPYKVDGEVVGTVGVIGPTRMAYERVVPIVDVTAKLLTSAL
                     SQH"
     gene            10120..10881
                     /locus_tag="VIG70_12945"
     CDS             10120..10881
                     /locus_tag="VIG70_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEY8251505.1"
                     /translation="MRDFLRKRGCEVLPEGETKADLAIVVGGDGTMLAAARELVRHRV
                     PLVGINLGRLGFMTDIGHDDMQAGVGAILDGKYAIEERSLLEAEIMRAGKSVLRTIAL
                     NEAVIGKGSQGRLIEFELSVDGEFVYRLRADGIIVSTPTGSTAYALSAQGPILHPAVP
                     ALTLVPLNPHTLSARPVSVSDRSVIEIMMVHALDARAHFDGLALADMAEGDRLILKRS
                     ADVVRFVHPPGYRYFATLREKLGWSEAIEKRRSME"
     gene            10886..12526
                     /gene="recN"
                     /locus_tag="VIG70_12950"
     CDS             10886..12526
                     /gene="recN"
                     /locus_tag="VIG70_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY8251506.1"
                     /translation="MLRALEVRDFVIVEKAGIELDDGFTVLTGETGAGKSILVDAIEL
                     LVGGRGDAALVREGAERAELSAEFEVEKKSSLIDWLEEGGLAGDPGSVILRRTIDRAG
                     RSRCFLNGHAATLAQLKAAGEHLIDIHGQHEHQSLLRPAAQRALLDAHAECESLAEET
                     AGAYRAWKRLQAVADEAEKNFAQREAERLSIQEQVDEFKNLAPREGEWQQVSAEHTRL
                     QHGSSLLGGAQSALEALEESEGACVAQLAALASRLRALSDHDDRLKPVVELLESAEAQ
                     AGEAARTLRQYASRVELDPQALRETETRIEALHAAARKHRLKPEALPERFAELQRRLA
                     ELELAVNPEALRREVAAAQKNFEAVAKKLSAKRRSGAEALGKAVTAAMQQLAMAGGRL
                     SVSLQELPEPGAAGLEGIELEVASHPSLPLRPLAKVASGGELSRVSLAIQLVAAKASS
                     VGSLVFDEIDAGIGGAVAETIGRSLRQLGKRRQVLCVTHLPQVAASGNHQWSVTRSGV
                     KVKVAQLDRGARIAELARMLGGAEGTARKHAAELLDSG"
     gene            complement(12523..12948)
                     /gene="fur"
                     /locus_tag="VIG70_12955"
     CDS             complement(12523..12948)
                     /gene="fur"
                     /locus_tag="VIG70_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEY8251507.1"
                     /translation="MSTVQSLKDSGLKATLPRRKILELFETSKVRHLSAEDVYKALLA
                     EGIDVGLATVYRVLTQFEQAGLLARQHFETGKAVFELNQGGHHDHLVCLQCGRVEEFY
                     DPDIEKRQNEVARQRGFALHGHSLALYADCIKKNCPYRP"
     gene            12969..13313
                     /locus_tag="VIG70_12960"
     CDS             12969..13313
                     /locus_tag="VIG70_12960"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEY8251508.1"
                     /translation="MRLGLFLIPVILLSGCGYVPRIPGVTPFRIEIQQGNYISQEMVA
                     QLKPGMSKEQVRLALGTPLLADIFHADRWDYVYYRDKPGEKRDQRKLTVFFEDGKLMR
                     LDGDVVAAGATQ"
     gene            13343..14068
                     /gene="dapB"
                     /locus_tag="VIG70_12965"
     CDS             13343..14068
                     /gene="dapB"
                     /locus_tag="VIG70_12965"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY8251509.1"
                     /translation="MGRTLIDAVLADGELSLVAALEMPKSPVLGTQAGGIKITADLAA
                     VAAADAVLDFTRPEGTLALLDRARALVIGTTGFTEEDKDRIAEAAKRIPIVMAANFAV
                     GVNTVYKLAETAARILGDAYDVEIVEAHHRHKVDAPSGTALELGRIVARTLGRDLGKT
                     ASYGRKGDVRERAAKEIGFHSIRGGDIVGEHTAIFAGEGERVEVTVRSQSRLTYAHGA
                     LRAAKWLRGRPPALYAMSDVLGL"
     gene            14131..15276
                     /gene="carA"
                     /locus_tag="VIG70_12970"
     CDS             14131..15276
                     /gene="carA"
                     /locus_tag="VIG70_12970"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY8251510.1"
                     /translation="MANFEPAALVLADGTAFRGRSIGARGTAVGEVVFNTAITGYQEI
                     LTDPSYCRQIVTLTYPHIGNTGVNEEDQESRRTYAEGLVVRDLPRLHSSWRSQGDLSS
                     YLNKNNVVGIAEIDTRKLTRILREKGAQNGCLMGGIVDEAKALDEARRFPGLAGMDLA
                     KVVTTDEPYAWTEGSWRWGKDGGSGYRNAENPRSHVVAYDYGIKRNILRMIVDRGARV
                     TVVPAQFPAREVLRMKPDGVFLANGPGDPEPCDYAIEAIGEILDATRIPVFGICLGHQ
                     LMGLASGAKTLKMKFGHHGANHPVKDLDSGRVVITSQNHGFAVDPSSLPSNLRPTHVS
                     LFDGSLQGLTRTDRPAFCFQGHPEASPGPHDIGYLFDRFAKMMDERA"
BASE COUNT         2471 a         5017 c         5198 g         2671 t
ORIGIN      
        1 aacacgggct gcgcccgccc gtcgaagagc gcatccagcg gagaagggtt ttgcttcaaa
       61 tattgatggc cctggcgtcc gctgcccgga accaccatga gaggcccgtt ttcctcgttc
      121 atgacgtcga ccgccatgag cgtctgcaca aaactgccgc ggccattcac gtccgtccac
      181 tggtcggtcc cgtagcggcg atggtcgcta tcctgatgcc acccgaatcc caccccatcg
      241 gcgggcagtt tgaagtgggc ctggcagatg agctgctcca tttcattgat tcccaggagt
      301 tggctcgaag gaaacgtcag ccgcgcgtcc tcgccgtact tcagaaggac cggctcggcc
      361 gctccacacc agacaatgcg attgattcgg tggtcattga cgacgaagta agagcctttg
      421 atcatcttgg gccgcgtcag cttgatcgcc gtgtcgcgaa ggcgatcaaa agccttgccg
      481 atttcccgga tttcagcgtc gcgaaagact ccgggcagca cgacatagcc tgcgtcgtaa
      541 aactcctcaa tctgggattt ggtcaggcaa agcatgtgtc cctgtttttt ctgattttgt
      601 cccctgaccc cgagtttcta cccgtcgaag aactccttga ccttgtcgaa ccagcccttg
      661 gcgcgcgggt tgtggcgcgc ggcgtcctgg gcgctgatcc cttcgaactc gcgcagcagc
      721 tgacgctggc gctcggtcag gctgaccggg gtctcgacca cgacgtggca gaagaggtcg
      781 ccggggaact ggctgcgcac gcccttgatg cccttgctct tcaggcgaaa ggtcttgccg
      841 ctctgggttt ccgccgggat gcggatcttg gccgagccgt cgagggtcgg gatggatatt
      901 tcgccgccga gcgcggcggt ggtgatcgag atcggcattt cgcagtgcag gtcgtcgtgg
      961 tcgcgctgga acacgctgtg cggcttgatg tgcacctgca cgtagaggtc tccgggcggg
     1021 ccgccgttca cgccgggctc gccttcgcct gacaggcgca cgcggtcgcc ttcgtcgacg
     1081 ccggccggga ttttcaccgc cagcgtcttg tgcagcttga tgcgcccggt ccccgcgcac
     1141 gggccgcacg ggttgggcac gacgctgccg ctgccgtggc agcgggggca ggtctgggcg
     1201 atcgagaacg ggccctgcga gacgcgcacc tgtccggcgc cgcggcacga agggcaagac
     1261 gcaggctgcg tgccggggcg cgcgccgctt cccttgcagg tctcgcacac cgcggcggag
     1321 gggatgcgga tcttggtctc gaagccgtgc gccgcctgct cgagcgcgag ctccaggttg
     1381 tagcgcaggt ccgcgccgcg gaacacggtg ctgcggccgg cgcgggcgcc gccgaagatg
     1441 tcgctgaaga tgtcgctgaa gatgtcgccg aagccggcgc cgggcccgcc gccggcctgc
     1501 ggcgtgacgc cggcgtggcc gaactggtca taggcggcgc gcttgttggc atcggccagg
     1561 atctcgtagg cctccttcgc ctccttgaag tgctcctcgg ccttcgggtt gtccggattg
     1621 cggtcgggat gccacttcat ggcgagcttc cgatacgact ttttcaggtc gtcatcgctc
     1681 gcgtccttgt tgacgccaag gacttcgtag taatcccgct tcgccaaaat cagctacccg
     1741 ttttcttgtc cttgacctcg gtgaactcgg cgtccacgac gttctcgtct ttcttgccgg
     1801 gttgctccgc ctgcccctgc gccgaccctg ctgcgctgcc cgcggcggcc tcggccgccg
     1861 ctttctgctg gtcggcgtac atcttctggc cgagggtctg cgcggcctgc gccagcgcct
     1921 ggctcttcgc gtcgatctca gccttgtcct cggacttgag cacgccctcg gcgtccttga
     1981 tcgcggcctc gatcttctct ttctcggccg cgtcgagctt gtcaccgtag tccttgagcg
     2041 attttctcac ggagtggacc agcgcctcgg cctggttccg cgcgttcacc agctcgaggg
     2101 tcttgcggtc ttcctcggcg tgcgcctcgg cgtccttcac catgcgctgg atctcggcct
     2161 cggtgaggcc ggagctcgcc tggatcttga tcttgttctc cttgccggtc gccttgtcct
     2221 ttgccgtgac gtgcaggatg ccgttggcgt cgatgtcgaa ggtgacctcg atctgcggca
     2281 tgccgcgcgg cgccggcggg atgtcggtga ggttgaactg gccgagcgac ttgttgccgc
     2341 cggccatgtc gcgctcgccc tgcagcacgt ggatggtcac cgcgttctgg ttgtcgtcgg
     2401 cggtcgagaa cacctgctgc gccttggtgg ggatggtggt gttcttctgg atcagcttgg
     2461 tcatcacccc gccgagcgtc tcgatgccga gagaaagggg cgtgacgtcg aggagcagca
     2521 cgtccttcac gtcgcccttc agcacgccgg cctggatcgc ggcgccgatg gcgaccgcct
     2581 cgtccgggtt cacgtccttg cgcggctcct tgccgaagaa ctcgcgcacc ttgtcgatca
     2641 ccttgggcat gcgcgtctgg ccgcccacca ggatcacgtc ctggatgtcc ttcgcgctga
     2701 tgccggcgtc cttgatcgcg atgccgcagg gcgcgatggt cttctcgatc aggtcgtcga
     2761 ccagcgactc gaacttggcg cgcgtgatct tcatgttgag gtgccgcggc ccgctctggt
     2821 cggccgtgat gtagggcagg ttgatctcgg tctgctgcga ggacgagagc tcgatcttcg
     2881 ccttctcggc ggattccttg agccgctgca gcgcgagcac gtccttcgac aggtccacgc
     2941 cctggtcctt cttgaactcg gacacgatgt agtcgatcag gcgctggtcg aagtcctcgc
     3001 cgccgaggaa ggtgtcgccg ttggtggaga gcacctcgaa ctgcttctcg ccctcgacgt
     3061 ccgcgatctc gatgatcgag atgtcgaagg tgccgccgcc caggtcgtac accgcgatct
     3121 tctggtcctt cttggcgatc ttgtcctcgc cgaaggcgag cgcccctgcg gtgggctcgt
     3181 tgatgatgcg cttcacgtcg agcccggcga tgcggccggc gtccttggtc gcctggcgct
     3241 gcgagtcgtt gaagtaggcg gggaccgtga tcaccgcgtc cttcacttct tcgccgaggt
     3301 agtcctcggc ggtcttcttc atcttgcgca ggatgtgcgc cgagacctcc tgcggcgaga
     3361 tcttcttgcc gcgcacttcg atccacgcgt cgccgttgtc cgcgcgcgcg atcttgtagg
     3421 gcatcagctt gatgtccttc tgcgtctctt tttcgtcgaa cttgcggccg atcaggcgct
     3481 tcaccgcgta gatggtgttc ttggcgttgg tgaccgcctg gcgcttggcc gaggcgccga
     3541 ccagcacctc gccgtcgtcg gtgaaggcga caaccgaggg ggtagtgcgg gcgccttccg
     3601 agttctcgat caccttgggc ttgccgccct ccatgatcgc gacgcaggag ttggtggttc
     3661 caaggtcgat gccgatgatc tttgccattt gagtccctag atttagtcgt ggcgtttcaa
     3721 tctaaccctg tgtctgagat ggggtttcca tctgattttt caagcgcttt ggccaccgtc
     3781 accaaggcgg ggcgaagcac ccggtcgtgc aggatgtagc ccttctgcat cacggaaacg
     3841 atggtgttcg gatcagcgtc ggcctcgacc gcggccatgg cctggtggcg gtgcgggtcg
     3901 aagcgctcgc tgggcttcgg atcgatctcg cggatgcgcg ctttctcgag cgccgagcgc
     3961 agcagtttca gggtgagctc gacgccgctg acgtcccggg tcttgagcgc cgcttccagg
     4021 ctgtccatca ccgggagcag gccctcggcg aagcgctcga gggcatactt ctgggcagtg
     4081 gcagcctcgg cttgggcgcg cttgcggccg ttctcggcgt cggccacggc ccgcatcatg
     4141 gtgtcgcgct gctgctcgat ccgggcgtgc gcctcggcca atagttcttc cagcgacttt
     4201 tcgttctggg gttgaacagt ctcttcctgc atcttgtcga attgggggtg gcgcggccgg
     4261 tttcaagccg cttaacggga cagattcgca gcataaaggc attgatttca tgaataaacc
     4321 tctcaagtac gggctgctcg gcctaggaac gctggtcgtg gtcgcggtgg cgggggcggt
     4381 ggtcttcgcg atgaccttcg atcccaaccg ctacaagggc gagatcgagc gcctggccaa
     4441 ggagaagacc gggcgcacgc tcgccatcaa gggcgacatc aagatggcgt tctggccgag
     4501 cctgggcgcc ggggtcgcgg gggtcagcct gacggaaaag ggctcgaacc aggcgttcgt
     4561 ctccttcgac aacgtgcatg cctcggtcaa gctgatgccg ctcctgagcg gccagtacat
     4621 cgtcgactcg gtgagcctcg ccgggctcaa ggcgcgcatc gtcaagcaga aggatggccg
     4681 cttcaacttc tcggacctga tggaagccga cgcaaagaaa cccgcggagc ccaagaagag
     4741 tcccgaggaa aaaaccggcc cggtggtctt cgacatcggc tcgatcagca tcgagcgctc
     4801 gtcgatcgcc tatctcgacc tccaggccgg gcaggaatac gcgctcgagg acgtgaagct
     4861 caagaccggc cgcatcgcgc aggacgccga aggcaagctc gaactcgcca tggtggcgaa
     4921 gcggaagagc ccgccgctgc aggcgaagct ctcggccgat ggcaagtacc agctcaaggg
     4981 cggggtgctg agcggcgacg tcacagccaa gctcgacgac tcgacgatca aggccaagtt
     5041 caccgccgcc gagccgtacg aattcgacgc agccatcgac aagatcaacc tcgatcgcta
     5101 tctcggggcg gcagaaaagc ccgcggcgca accccagaag caagagactg cgaaaagcaa
     5161 gcaggaagac accccggtcg atctctcggc cctcaagggc atcaacgcca agggcaagct
     5221 gcaggtcggc gcgctggaag tgcgcggcct gaagctcgcc gacgtgagcg cccagctcaa
     5281 cgccgcgaac ggcaaggtca ccgtggcgcc gcactccgcg aagctctacg aaggcacgat
     5341 ttccggggaa gtggcggtcg atgccaacaa gaaccaggtt gcgctgaagg agcagctgca
     5401 gaacgtttcc gtcggcccga tcctgcgcga cttcgccgag caggaccggc tcgaaggcaa
     5461 ggggaacgtc gcgctcgacg tcacgacatc tggggcgacg gtgaacgcca tgaagcgtgc
     5521 gctcaacggt acggcgaagg tgcagctgcg cgacggcgcc atcaagggca tcaatctggg
     5581 cgagatcctg cgcaaggcgc gcaccgcgct cggcggctcg cagtccgcgg ccgacgggca
     5641 gagcgccggc gacaagacgc aaagaaccga tttcagcgag ctcaacgcga gcttcgcgat
     5701 caagaacggg gtcgcgcaca acgaggacct cgacgtaagg gcgccgctct tccggctggg
     5761 cggcgccggc gacatcaaca tcgccgactc gagcctgaac tacctcgcga aagcgtcgct
     5821 cgtcgccacc tcgcagggcc agggcggccg cgatcgcgac actctcgccg ggctcaccgt
     5881 gccggtgcgg ctcacgggga cgttcgacga gcttaaatat gaagtggact accggggctt
     5941 ggcgggcgcg gcggccaagt cacaggtcgg cgggaaaatc aaggagcagc tcgaagaacg
     6001 cgtgttgaaa gatgaaaaat taaaagagag gttaaaggga ttgttaggac gctaggggtt
     6061 ccccccggtt agcctcagga agtccccgag tgcggggcgt atgcgcgcct cgtatgcttt
     6121 tgggttcgat gctcaacctc ctgcggtatt cgcagctgat cgcgcttgcg gtcctgtgcg
     6181 gcgtggtcgt cgccctgtcg ctgctttacc gcggcctgct gttcgactcg atgatcgaga
     6241 gcgagacgca cgcgagcgtc gcgctcacca agactttcgc caacgccgtg tggccttcgc
     6301 acgccgcgtt cgtcgggcgc gccggctcgc tcgatcgcgc cacgctcgcc ggccgcccgg
     6361 agatcgcggc gctcgaccac gacctgcggc gcctgtccga ggccctgccc gtggtcaaag
     6421 tgaagatcta cgacctccac ggcctgactg tttattccac cgacgcgagc cagatcggcc
     6481 aggacaaggc tgccaatccc ggcttcaagc gggcgcgcga cggctatgcg gcgagcaacc
     6541 tgactttccg cgagcgcatc gacagctggg agggcggcga gctcgcggat cgccacatcc
     6601 tcgcgaccta cgtcccggtg cacgtctacg atgccgcgcc ggtcgaggcg gtgctcgagg
     6661 tctataccga cgtcaccgag ctggtcgcgc gcatggagcg gggtcagtgg cagatcatcg
     6721 ccgtggtgct cggcgcgatg gcgctgatct atctgctgct gcagctcatc ctggcgcgct
     6781 accagcgcct gctggccgaa aaggagcgcg agcgcgccgc gcaggaggag cgcatccgcc
     6841 accaggccta tcacgatgcg ctcaccgggc tgcccaaccg cgcgagcttc accgagcacc
     6901 tcgaggaggc gatgcggcgc gccaagcgcg ccggctggcc gctcgcgatc ctgttcctcg
     6961 acctggacct cttcaagcgc gtcaacgaca gcctcgggca cgacgccggc gaccgcctgc
     7021 tgcgcgtcgc cgccgagcgc atccggcgcg ccgtgcgcga ggccgacatg ctgttccgca
     7081 tgggcggcga cgagttcacc gtgctgctcg aggacgtgcg cgggccggag gaggcggcgg
     7141 cggtggcggg ccgggtgctc gagggaatcg ccgagccgct gcagctccag caccacgagc
     7201 tcgcggtcac cgcgagcatc ggcatcgcgc tctatccgcg cgacgacgtg ctcggcgagc
     7261 gcctcgtgaa gagcgccgac accgcgatgt accgcgccaa ggagctcggc cgcaaccgtt
     7321 tcgcgttctt cgcgaaagag atgaacgagc gcgtcgagag ccagatcatg atcgagaccg
     7381 cgctgcgccg cgcgctcaag aacgacgagt tcgtgctgca cttccagccg cgcgtgtcgg
     7441 cggcgaccgg ccgcgccacc ggcgcggagg cgctgctgcg ctggaagcat cccgagtggg
     7501 ggctggtcga gccggcgcgg ttcgtgccgc tgctcgagga gaccgggctg atcgtgccgg
     7561 tcggtaactg ggtgctgctt gaagcctgcc gccaggcgat ggcgtggcag gccgcgggcc
     7621 tgccgccgct gcgcgtctcg gtgaacctgt cctcgcgcca gttccgcagc gaggcgctgt
     7681 tcgaggcggt gagcgaggcg ctgcgcgcga gcaagctggc gccgcagctc ctcgacctcg
     7741 agctcaccga gagcctgctg atcgacaacg tcgaccacgc gatgggcgtg atgggcaagc
     7801 tcaaggcgat cggcaccgcg atctcgatcg acgacttcgg caccggctat tcctcgctcg
     7861 gctacctcaa gcgcttcccg atcgacagcc tgaagatcga caagagcttc gtgcgcgaca
     7921 tcgccaccag ccccaaggat gcggcgatcg tcaacgcgat ctccgcgctc gcgcgcagcc
     7981 tgggcatcgg gctggtcgcc gagggcgtcg aggagccctg gcaggtcgag ttcctgcgcg
     8041 cgcgccactg caccgaaatg cagggctatc tcttcagccg cgcgcttccc gccgagtcgc
     8101 tcgccgcggc gctgtcgcgc tcctacccga tgccgcgccc cagcgcggcg agcgcgtcga
     8161 tccagtccgg agactcgttg aggcagttca cgaggtagaa ctcgcgcccg ccggcggcga
     8221 ggaatgcgcg ccgcgcgacg atgccgatct cttccagcgt ttccaggcag tcggagacga
     8281 agcccgggca taacacgtcg actctgccag ttccctttct tgcaaggtcg gtgagcgtag
     8341 gctcggtata gggctgcagc cactccgcat aaccgaagcg cgactggaac gtgacgaggt
     8401 attcagtctt caggcgctcg gcgagcagcc gcgctgtcgc gcggcattct ttctcgtagc
     8461 gctcggatcc ctttttcggc aggccgtggt agctcatgac gagccgggcg ccgcgcccgt
     8521 gctgctgcca gtgccggcgc acgttcgctg ccagggcggc gatgtacgcg ggatggtcgt
     8581 ggaagctctc gagcatcggc acgccccggg gcagcatgtc ggcgatgctg gccgtcgtgc
     8641 tttcggcata ttgcgggtag agcggaatga cgagcggctc ggccagattt tcgagacttg
     8701 ttcggatgcc gggttgccca taccgcatcg cgtatcgcac ggggatgccg agcttctcct
     8761 gcagaagcgc cgcctgccgc accgtgtgca ccgcgagcgg tgagccctcg gacgtccaga
     8821 tttgcgagta cttttcagcc gagcgcttgg ggcgcgttct gagaacaatg ccgtgaagga
     8881 tcgggagcca gagccagcgc ggcagcttga ccacgcgcgg atcgcccagg aactcggcga
     8941 gatagctctt taccgcctcc ggcgtcggcg ccgccggggt gccgagattg acgaggagga
     9001 cgcttttcag tgctgcgaca gggcgctggt gaggagcttc gcggtgacgt cgacgatcgg
     9061 cacgacgcgc tcgtaggcca tgcgcgttgg cccgatcacg ccgacggtgc cgaccacctc
     9121 gccgtccacc ttgtagggcg aggtcacgat gctcacgtcg tcgggggcga ccacgccgga
     9181 ctcgccgccg atgaagatct gcaccccctg gccgcgcagc gccaggtcga ggatcttgat
     9241 gagcgaggtc ttgcgctcga acagctccag cagctgcttc agcctcccca tgtcctgcga
     9301 aaggtcgcgc accgcgaaga ggttccgctc gccggagatc acgtactgct cgccgccctc
     9361 ggcgagcgcg ctgccggcgt cgagcgcggc gctcatcagc cgggtcatgt cctcgcgcac
     9421 ttcgcgcacc tcgccctgca ggcgcgcgcg cacgtcctcg aagctctgcc cggcgtagtt
     9481 ctggttgatg tagttcgccg cctcgatcag ctccgcgggc cggtattcca cctcggtgaa
     9541 caggatccgg ttctgcacgt cgccgtcggg cgtgaccacg atcatcagga tgcgcttctc
     9601 ggacagcctg aggaactcga tgtgcttgaa ggtcaccgcg cgccgcggcg tcatcaccac
     9661 gccggcgaac tgcgtgagct gcgacagcag ctgcgacgcc gcctggatca cgcgctgcgg
     9721 gttgtcgggg tgcagctcgc cctcgattcg cagcagcgca ttcgactcga gcggcttgat
     9781 cacgagcagc gtgtcgacga agaagcggta gccgagcggc gtcggcacgc ggccggcgga
     9841 ggtgtgcggg ctggcgatga agccgagctc ctcgagatcg gccatcacgt tgcggatcgt
     9901 ggcgggcgaa agatcgagcc cggagtactt cgacagcgtg cgcgagccga tcggctggcc
     9961 ctcggcaata tagcgctcga tcaacgtctt gagcaggatc tgagcgcgct tgtcgagcat
    10021 ggaattcatt gtacactcga gcgcatgccg gcttcgtttc gcagaatcct tctcatcggg
    10081 aagctgcgca gcgcggagat cgaatcctcc ctcagtgaat tgcgtgattt tctgcgcaag
    10141 cgcggttgcg aggttctccc cgaaggcgag accaaggccg acctcgcgat cgtggtcggg
    10201 ggcgacggca cgatgctcgc ggcggcgcgc gagctggtgc gccaccgcgt gccgctcgtc
    10261 ggcatcaact tggggcgcct gggcttcatg accgacatcg ggcacgacga catgcaggcc
    10321 ggcgtcggcg cgatcctcga cgggaaatac gcgatcgaag agcgctcgct gctggaggcc
    10381 gagatcatgc gcgcaggcaa atcggtgctg cgcacgatcg cgctgaacga ggcggtgatc
    10441 ggcaagggat cgcaggggcg cctgatcgag ttcgagctct cggtcgacgg cgagttcgtg
    10501 taccggctgc gcgccgacgg catcatcgtc tccacgccca ccggctcgac ggcctacgcg
    10561 ctgtctgcgc agggcccgat cctgcatccc gcggtgccgg cgctgaccct cgtgccgctc
    10621 aacccgcaca cgctgtcggc gcgcccggtg agcgtgagcg atcgcagcgt gatcgagatc
    10681 atgatggtgc acgcgctcga cgcgcgcgcg cacttcgacg gcctcgcgct cgccgacatg
    10741 gccgagggcg accgcctgat cctgaagcgc tcggccgacg tggtgcgctt cgttcatccg
    10801 cctggctacc gctacttcgc gacgctgcgc gagaagctgg gctggagcga ggcgatcgaa
    10861 aagcgccgca gcatggagta gacgcgtgct gcgcgctttg gaggtcaggg actttgtcat
    10921 cgtcgaaaaa gcgggcatcg agctcgacga tggctttacg gtgctgaccg gcgagaccgg
    10981 cgccggcaag tcgatcctgg tggacgcgat cgagctcctg gtcggcggcc ggggcgatgc
    11041 ggcgctggtg cgcgaaggcg ccgagcgggc agagctgtcg gccgaattcg aagtcgagaa
    11101 aaaatcgtcc ttgatcgatt ggctcgagga gggcggtctc gccggcgatc ccggcagcgt
    11161 catcctgaga cggaccatcg accgcgccgg ccgctcgcgc tgcttcctca acggccatgc
    11221 cgcgaccctc gctcagctca aggccgcggg cgagcacctg atcgacatcc acggccagca
    11281 cgagcaccag tcgctgctgc gcccggccgc gcagcgcgcg ctcctcgacg cccacgcgga
    11341 atgcgaatcg ctggcggagg aaaccgccgg ggcctaccgc gcgtggaagc gcctgcaggc
    11401 cgtggcggac gaggccgaga agaacttcgc gcagcgcgaa gcggaacgtc tttccatcca
    11461 agagcaggtt gatgaattca agaatcttgc tccccgtgaa ggcgaatggc agcaggtctc
    11521 ggccgagcac acgcgcctgc agcacgggtc gagcctcctc ggcggtgcgc agtccgcgct
    11581 cgaagcgctg gaagaatccg aaggcgcatg tgtggcgcag cttgctgcgc tggcgagccg
    11641 gctgagggca ctctcggatc acgacgaccg gctgaagccg gtcgtggagc tgctcgaatc
    11701 cgccgaggcg caggcagggg aggcggcgcg cacgctgcgc cagtacgcct cgcgcgtcga
    11761 gctcgacccg caggcgctgc gcgagacgga aacgcgcatc gaggccttgc acgcggctgc
    11821 ccgcaagcac cggctcaagc cggaagcgct ccccgagcgc tttgccgagc tgcagcggcg
    11881 cctggcggag ctcgagctcg cggtcaatcc cgaggcgctg cggcgcgaag tggccgctgc
    11941 gcaaaagaac tttgaggccg ttgcgaaaaa actctccgcc aagcgccgga gcggcgccga
    12001 ggcgttgggc aaggccgtga ccgcggccat gcagcagctc gccatggccg gcggccgcct
    12061 ctcggtttct ctccaggagc tgccggagcc cggcgcggcc ggcttggagg ggatcgagct
    12121 cgaggtggcc tcgcacccga gcctgccgct gcgtccgctc gccaaggtcg cctccggcgg
    12181 cgagctgtcg cgcgtcagcc ttgcgatcca gctcgtggcg gcgaaggcgt cgagcgtcgg
    12241 gtcgctggtc ttcgacgaga tcgacgccgg catcggcggc gcggttgccg agaccatcgg
    12301 cagatcgctg cgccagctcg ggaagcgccg ccaggtgctg tgcgtcacgc acttgccgca
    12361 ggttgccgcc agcggcaacc atcagtggag cgtgacacgc tcgggcgtga aggtgaaagt
    12421 cgcgcagctc gaccgcggcg cgcgcatcgc ggagctcgcc cgaatgctcg gaggtgcgga
    12481 aggtacggcg cggaagcacg ccgccgaact actggattcc ggctagggcc ggtaggggca
    12541 gttcttcttg atgcagtctg cgtacagggc gagcgagtgc ccgtgcagcg cgaagccgcg
    12601 ctggcgcgcg acctcgttct ggcgtttctc gatgtcggga tcgtagaact cctcgacgcg
    12661 gccgcattgc aggcagacga ggtggtcgtg gtgcccgccc tggttcagct cgaacaccgc
    12721 cttgccggtc tcgaagtgct ggcgcgcgag caggccggcc tgctcgaact gcgtcagcac
    12781 gcggtaaacc gtggcgaggc cgacgtcgat gccctcggcg aggagcgcct tgtagacgtc
    12841 ctccgcggac aggtggcgca ccttgctggt ttcgaagagc tccaggatct tacgccgggg
    12901 cagggtcgcc ttgaggccgc tgtccttcag gctttgcacc gtgctcatgc ggctgtcatt
    12961 atatgcgcat gcgtttgggg cttttcctga ttccggtcat tctcttgagc ggttgcggct
    13021 acgttccgcg gattccgggc gtgacgccct tccgcatcga gatccagcag gggaattaca
    13081 tctcgcagga gatggtggcg cagctcaagc ccggcatgag caaggagcag gtgcggctcg
    13141 ccctcggcac gccgctgctc gccgacatct tccacgccga ccgctgggac tacgtctact
    13201 accgcgataa gcccggcgaa aaacgcgacc agcgcaagct caccgtgttc ttcgaggacg
    13261 gcaagctcat gcgcctcgac ggcgacgtgg tcgccgccgg cgcaacgcag tgaagatagc
    13321 catcgcgggc gcgggcggcc gcatgggccg cacgctgatc gacgccgtgc tcgccgacgg
    13381 cgagctctcc ctcgtagccg cgctggagat gccgaagagc cccgtcctcg ggacgcaggc
    13441 gggcggcatc aagatcacgg cggacctcgc ggcggttgcg gcggccgacg cggtgctcga
    13501 cttcacgcgg ccggaaggca cgctcgcgct cctcgatcgc gcccgggcgc tggtgatcgg
    13561 caccacggga ttcaccgaag aggacaagga ccggatcgcg gaggcggcaa agcgcatccc
    13621 gatcgtgatg gcggcgaact tcgccgtcgg cgtgaacacc gtctacaagc tcgcggagac
    13681 cgcggcgcgg atcctgggcg acgcgtacga cgtcgagatc gtcgaggcgc accaccggca
    13741 caaggtcgac gcgccctccg gcaccgccct ggagctggga agaatcgtgg caagaacgct
    13801 gggtcgggat ctcggcaaga ccgcctccta cgggcgcaag ggcgacgtgc gcgagcgcgc
    13861 cgcgaaggag atcggcttcc actcgatccg gggtggcgac atcgtcggcg agcacaccgc
    13921 gatcttcgcc ggcgagggcg agcgcgtcga agtcacggtg cgctcgcaaa gccgcctgac
    13981 ctacgcgcac ggcgcgctgc gcgcggcgaa atggctcagg ggacggccgc ccgcgctgta
    14041 cgccatgagc gacgttttag gcctataatc ccgtcttcaa tgaagcggga agggcaaatg
    14101 cctttcccgc ttttcgcatg cgcttcaaaa atggctaact tcgaacctgc cgcgctggtc
    14161 cttgccgacg ggacggcctt tcgcgggcgc tcgatcggcg cgcgcggcac cgcggtgggc
    14221 gaggtggtgt tcaacaccgc catcacggga taccaggaga tcctcaccga cccgtcctat
    14281 tgccggcaga tcgtgacgct gacctatccg cacatcggca ataccggggt caacgaggaa
    14341 gaccaggaat cgcgccgcac ctatgccgag gggctggtgg tgcgcgacct gccccggctt
    14401 cactcgagct ggcgctcgca aggcgacttg agctcctacc tgaacaaaaa caacgtggtc
    14461 ggcatcgccg agatcgatac ccgcaagctg acgcgcatcc tccgcgagaa gggcgcgcaa
    14521 aacggctgct tgatgggggg catcgtggac gaggcaaaag cgctcgacga ggcgagaagg
    14581 tttccgggcc tcgccgggat ggacctcgcc aaggtggtga cgaccgacga gccgtacgcc
    14641 tggaccgagg gaagttggag gtgggggaaa gacggtggct cggggtaccg caatgccgag
    14701 aacccgcggt cgcatgtggt cgcgtacgac tacggcatca agcgcaacat cctgcgcatg
    14761 atcgtcgatc gcggggcgcg ggtcaccgtg gtccccgccc agttcccggc ccgggaagtc
    14821 ctgcggatga agcccgacgg cgtattcctc gccaacggcc cgggcgatcc cgagccctgc
    14881 gactacgcga tcgaggcgat cggcgagatc ctcgatgcga cccggatccc ggtgttcggc
    14941 atctgcctcg gccaccagct catggggctc gcctcgggcg cgaagacgct caagatgaag
    15001 ttcggccacc acggcgcgaa ccatccggtg aaggacctcg attccggccg cgtcgtgatc
    15061 accagccaga accacggctt tgccgtcgat ccttcctctt tgccttcgaa tctgagaccc
    15121 acccacgtgt ctctgttcga cggttccctc caggggctga cgcgcactga tcggccggcc
    15181 ttttgttttc aaggacatcc ggaggcgagc ccgggtccgc acgacatcgg gtatctgttc
    15241 gaccgcttcg ccaagatgat ggacgagcgt gcctaagcgg accgacctga aaagcatcct
    15301 gatcatcggc gcggggccga tcgtgatcgg ccaggcgtgc gagttcgact actcggg
//
LOCUS       DATBSZ010000201         4765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_580870, whole genome shotgun sequence.
ACCESSION   DATBSZ010000201 DATBSZ010000000
VERSION     DATBSZ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580870"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VIG70_12975"
     CDS             complement(<1..1394)
                     /locus_tag="VIG70_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8251511.1"
                     /translation="MAKPQVYDASAIQALKGLEPVRRMPGMYTHTVHPLHVVQEAIDN
                     AVDEALAGFGKRITVSVLKDGSVQVEDEGRGVPVDMHPVEKKPAVEVAFTMLHAGGKF
                     SRSGDGVYHISGGLHGVGVAVSNALSKRCEVTVFRDGQKHTIVFEGGELKSRLKSEKI
                     KERKTGTIVRLWPDPKYFDSPNIPMNELEHLVRSKAYLLPGLTMALEVEGKQTRLWKY
                     ERGMAQYFDFMLDGRELVAPLFTGEKFFDEKATKDITDIEPGEGASWAIGWVAEGDIF
                     ADSHVNLIPTRSGGTHEAGFRAGIFEALGAFMDARALAPKGVKLIPDDLWQRACFTLS
                     ARIVRTQFHGQTKEKLTTRHAARLLEMCVRDAFELWLNEHVDEGKKIVALAIEQAMER
                     LAKGKKVERKKSSGIATLPGKLVDCASGDIQRNELFLVEGDSAGGSAKEARDKETQAL
                     LPLRGKVLNTMSKD"
     gene            complement(1448..1906)
                     /locus_tag="VIG70_12980"
     CDS             complement(1448..1906)
                     /locus_tag="VIG70_12980"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY8251512.1"
                     /translation="MPAVLDASVTLPWFLQDERTAFTDSVFAALGRDEFWVPAVWRLE
                     FPNALLAAERKRRIDRQTRIEALEFASGLALRVDPAPIDMRSISAIAERHGLSTYDAA
                     YLELAARQGIGLITLDRALADAAAAAGIAVQSPGRSTAAQTRRRYAAQAA"
     gene            complement(1906..2160)
                     /locus_tag="VIG70_12985"
     CDS             complement(1906..2160)
                     /locus_tag="VIG70_12985"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY8251513.1"
                     /translation="MTKVGSFEAKTKLAELLDKVEAGETVTITRRGVAVAQLVPVKAV
                     DERTRQRALIEEIKRWRVGKDRGAKPGSTIPELIKAGRRY"
     gene            complement(2215..2895)
                     /locus_tag="VIG70_12990"
     CDS             complement(2215..2895)
                     /locus_tag="VIG70_12990"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8251514.1"
                     /translation="MADVEREGPVARIFLNRPEKANALNSAALRELASSLESLAPDES
                     LRVVVLAGRGKTFCGGADVHELKALTAATAGAFVEKIHNVCAAIRELPVPVVARLHGA
                     VIGAGLEIAAACDLRVAAEGTKFAMPEVRLGIPSVVEAALLPRLMGSGRAAWLVLTGE
                     AIDARRALEWGLIEEIGDGEQLLQRLRAGDREALRMQKQLLHLWDEASLATSVAASIE
                     RFAQVHRR"
     gene            2894..3598
                     /locus_tag="VIG70_12995"
     CDS             2894..3598
                     /locus_tag="VIG70_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HEY8251515.1"
                     /translation="MLSSYSREALKKTSTATLTTVLFKRGLRNVFIQGIFLLNRDAPR
                     MVGEAYTLRYIPAREDVDQLGAFEGRGHPQREAIEACPAGSVLVMDARRDASAATGGD
                     ILMTRLMVRGVSGVVTDGGLRDSPTIERLAWPAYCGARSAPLNLVRHHAVESQVPIGC
                     GGVAVYPGDVVVGDAEGVVVIPASMAEEVAKEAEAQTEFEDWVEARVKEGRSIFGLYP
                     PNAETKAEFEAFRRKK"
     gene            3734..>4765
                     /locus_tag="VIG70_13000"
     CDS             3734..>4765
                     /locus_tag="VIG70_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8251516.1"
                     /translation="MSFRKLIAVLLFAALAAPAAAQETIVVYFQKGFYPAEDKAVDEL
                     VDKFQKKTGVKVELSRYAPQEMIPKTVAALDAGTPPDMVYGDVFDFQVAGKWAFEGRL
                     EDVSDIINPMKGKFLPNTIETTFMYNDKEKKRAYYAFPVKRQTMHIQYWKDMLADAGY
                     KESDIPKTWKAYFDFWCTKVQEGYRKKTGKRVYSVGSPMGVDSSDSFYSFLTYMDGYN
                     VRLVNDDGKLNLGKDTRAGLINAVRDYTETIGKGCTPPSAINWKDPDNNVNFHNKTII
                     MTHNATISIAAKWLDDMNNDKLKPEERAQAKTNYEKLIATAGVPNKPDGKPMQYRAAV
                     KVGVVFTAAK"
BASE COUNT          829 a         1599 c         1517 g          820 t
ORIGIN      
        1 ctgtccttcg acatggtgtt cagcaccttg ccgcgcagcg gaagcagcgc ctgcgtctct
       61 ttgtcgcgcg cctctttggc cgagccgccg gcggagtcgc cttccaccag gaacaattcg
      121 ttgcgctgaa tatcgcccga ggcgcaatcc accagcttgc ccggcagcgt ggcgatgccg
      181 ctcgacttct tgcgctcgac cttcttgccc ttggcgaggc gctccatcgc ctgctcgatc
      241 gcgagcgcca cgatcttctt gccttcgtcg acgtgctcgt tcagccacag ctcgaacgcg
      301 tcgcgcacgc acatctcgag caggcgcgcg gcgtggcgcg tggtgagctt ttccttggtc
      361 tggccgtgga actgcgtgcg cacgatgcgc gcggagagcg tgaagcaggc gcgctgccag
      421 agatcgtcgg ggatcagctt cacccccttg ggcgcgaggg cgcgcgcatc catgaacgcg
      481 ccgagcgcct cgaagatgcc ggcgcggaag ccggcctcgt gcgtgccgcc cgagcgcgtc
      541 gggatcaggt tgacgtggct gtcggcgaag atgtcgccct cggcgaccca gccgatcgcc
      601 cacgaggcgc cctctccggg ctcgatgtcc gttatgtcct ttgttgcctt ttcatcaaaa
      661 aacttctctc ccgtaaaaag cggggccacc agctcgcgcc cgtcgagcat gaagtcgaaa
      721 tactgcgcca tgccgcgctc gtacttccac agccgggtct gcttgccctc gacctcgagc
      781 gccatcgtca ggccgggcag gagatacgct ttcgaccgga cgagatgttc gagctcgttc
      841 atcgggatat tcggcgagtc gaagtacttg ggatcgggcc agaggcgcac gatggtgccg
      901 gttttccgct ctttgatttt ttcgctctta agacgggatt tgagttctcc cccttcgaaa
      961 acgatcgtgt gcttctgccc gtccctgaac accgtcactt cacagcgctt cgacagcgcg
     1021 ttcgacaccg cgacgccgac gccgtgcagt ccgccggaga tgtggtagac cccgtcgccc
     1081 gagcgcgaga acttgccgcc ggcgtgcagc atggtgaacg cgacctcgac cgcgggcttc
     1141 ttctcgaccg ggtgcatgtc gaccggcacg ccgcggccct cgtcctcgac ctggaccgag
     1201 ccgtccttga ggacgctcac cgtgatgcgc ttgccgaagc cggcgagcgc ctcgtcgacc
     1261 gcgttgtcga tcgcttcctg cacgacgtgc aggggatgca cggtgtgggt gtacatcccc
     1321 ggcatgcgcc gcaccggctc gaggcccttg agcgcctgga tcgccgatgc gtcgtagacc
     1381 tgcggcttag ccatagattt agttcagttt atctagccgt ccacaacata ttgtagtccg
     1441 cggcggttca cgctgcctgc gccgcgtagc ggcggcgcgt ttgcgccgcc gtcgaccggc
     1501 ccggcgactg caccgcgatg ccggcggccg ctgccgcatc cgccagcgcg cggtccagcg
     1561 taatcaggcc gatgccctgg cgcgcggcga gctcgaggta agcggcgtcg taggtgctca
     1621 agccatgccg ctcggcgatc gcgctgatcg atcgcatgtc gatcggcgca gggtcgacgc
     1681 gcagcgccag cccgctcgca aactcgagcg cctcgatgcg cgtctgccgg tctatccggc
     1741 gcttgcgctc tgccgcgagc agcgcattgg ggaactcgag ccgccacacg gccggcaccc
     1801 agaactcgtc ccggccgagc gccgcgaaca ccgaatccgt gaacgcagtg cgctcatcct
     1861 gcaggaacca cggcagcgtg acggacgcgt cgagcaccgc cggcatcagt agcgcctgcc
     1921 cgccttgatg agctccggga ttgtcgatcc cggcttcgcg ccgcggtctt tgccgacccg
     1981 ccagcgcttg atttcctcga tcagcgctcg ctgccgcgtc cgttcgtcga ccgctttgac
     2041 cggcacgagc tgcgctaccg cgacgcctcg ccgcgtgatc gtcacggttt ccccagcctc
     2101 gaccttgtcg agcagctcgg cgagcttggt tttcgcttca aaggatccga ccttggtcat
     2161 acggagacgc tccaactaga caaccagttg gaacgatact acaaccgggc gcctttatct
     2221 acggtggacc tgcgcaaagc gctcgatgct cgccgccacg gacgtggcaa gggacgcctc
     2281 gtcccagagg tgcaggagct gcttctgcat gcgcaacgcc tcgcgatcgc cggcccgcag
     2341 gcgctgcaac aactgttccc cgtcgccgat ctcttcgata agcccccatt ccagcgctcg
     2401 gcgcgcgtcg atcgcttcgc cggtcagcac cagccacgct gcgcgcccgg agcccatcag
     2461 ccgcggcagg agcgcggcct cgaccaccga cgggatgccg agcctgacct ccggcatggc
     2521 gaatttcgtg ccctctgcgg cgacgcgcaa atcgcaggcc gcggcgatct ccaggcctgc
     2581 gccgatcacc gcaccgtgca gccgggccac cacgggaacc ggcagctcgc ggatcgcggc
     2641 gcaaacgttg tggatctttt cgacgaacgc gcccgcggtg gcggcggtca gggctttcaa
     2701 ctcgtgcacg tcggcgccgc cgcagaacgt cttgccgcgc ccggcgagca ccaccaccct
     2761 gagggactcg tccggggcga gcgattcgag gctgctcgcc aattcccgca aagctgcgga
     2821 attcaaagcg tttgcttttt ccggccggtt gaggaagatt cgggcgaccg gaccttcgcg
     2881 ctccacatcc gccatgcttt catcttatag ccgcgaggct ttgaaaaaga cctccactgc
     2941 gacgctgacc accgtgcttt tcaagcgcgg cctgcgcaac gtcttcatcc aggggatttt
     3001 tcttctgaac agggacgccc cgcggatggt cggcgaggcc tacaccctgc gctacatccc
     3061 ggcgcgcgag gacgtcgacc agctcggcgc cttcgagggg cgcgggcatc cgcagcgcga
     3121 ggcgatcgag gcgtgcccgg cggggagcgt gctggtgatg gacgcgcggc gcgatgcgag
     3181 cgcggccacc ggcggcgaca tcctgatgac gcggctcatg gtgcgcggcg tgtcgggcgt
     3241 ggtgaccgac ggcggcctgc gcgactcgcc gacgatcgag aggctggcgt ggccggcgta
     3301 ttgcggcgcg cgctcggccc cgctaaacct ggttcggcac cacgcggtgg agagccaggt
     3361 gccgatcggc tgcggcggcg tcgcggtcta tcccggcgac gtggtggtcg gcgacgccga
     3421 gggcgtggtc gtcatcccgg caagcatggc cgaagaggtg gcgaaggaag ccgaggcgca
     3481 aaccgagttc gaggactggg tagaggcccg ggtgaaggaa gggcgctcga tcttcgggct
     3541 ctacccgcct aatgccgaga cgaaggcgga gttcgaggcg ttccgcagga agaaatagaa
     3601 ttcggggtca gggtgcgaat tcggtttgcg cgtacgcaca aacgaattcg caccctggcc
     3661 ccgaattctg taggtaacct tccagttact ttgtcagtta tcatacaagc gggtacctag
     3721 aggaggcctt cccgtgtcat ttcgaaagct gatcgctgtt ctgctgttcg ccgcgctcgc
     3781 ggcgcctgcc gcagcgcagg aaaccatcgt ggtgtacttc cagaaggggt tctatcccgc
     3841 cgaggacaag gcggtcgacg agctggtcga caagttccag aagaagaccg gcgtgaaggt
     3901 cgagctgtcg cgctacgcgc cgcaggagat gatcccgaag acggtcgccg cgctcgacgc
     3961 cggcacgccg cccgacatgg tgtacggcga cgtgttcgac ttccaggtcg ccggcaagtg
     4021 ggccttcgag gggcgcctag aggacgtgtc cgacatcatc aacccgatga agggcaagtt
     4081 cctgccgaac accatcgaga ccaccttcat gtacaacgac aaggagaaga agcgcgcgta
     4141 ctacgccttc cctgtcaagc gccagaccat gcatatccag tactggaagg acatgctggc
     4201 cgacgcgggc tacaaggagt cggacatccc gaagacgtgg aaggcgtact tcgacttctg
     4261 gtgcaccaag gtgcaggagg gctaccgcaa gaagaccggc aagcgcgtct actcggtggg
     4321 cagcccgatg ggcgtggact cctccgactc gttctactcg ttccttacct acatggacgg
     4381 ctacaacgta agactggtga acgacgacgg caagctcaac ctcggcaagg acacgcgcgc
     4441 cggcctgatc aacgcggtgc gcgactacac cgagaccatc ggcaagggct gcacgccgcc
     4501 ctccgccatc aactggaagg accccgacaa caacgtcaac ttccacaaca agacgatcat
     4561 catgacgcac aacgcgacga tctcgatcgc cgcgaagtgg ctcgatgaca tgaacaacga
     4621 caagctcaag cccgaagagc gggcgcaggc gaagaccaac tacgagaagc tgatcgccac
     4681 cgccggcgtc ccgaacaagc ccgacggcaa gccgatgcag taccgcgcgg ccgtcaaagt
     4741 gggcgtggtg ttcaccgctg ccaag
//
LOCUS       DATBSZ010000202         2689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1059613, whole genome shotgun sequence.
ACCESSION   DATBSZ010000202 DATBSZ010000000
VERSION     DATBSZ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059613"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1557)
                     /locus_tag="VIG70_13005"
     CDS             complement(<1..1557)
                     /locus_tag="VIG70_13005"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY8251517.1"
                     /translation="MIGMNDFELLEFKISRRAFMGGSAGLAFAFTLGVGKEAKAQTPG
                     TLNAYVSIAPDGTITIQQPVAEMGQGISTGLPLIVAEELDADWSKVKVVQSPIAPAYH
                     HPIFKAQYVVASISTLGYWTPMRMAGAQARRVLVDAAATKWGVPAAECVTEPSTVVHQ
                     PSGRKMSYGEIASFATPPATPPAIDATKDLKKPGDYRLLGKGVPRVDVPAKATGAAKY
                     GIDARVPGMAYATFVRSPVRGSGPASSNAADVKKQPGIVDVVTLDHGVAVVGETFAAV
                     SKARKQLKVTWRSGQPGDAINTDKDLETYLGDGRDPKRVGVEWKSKGDPAKAIGGAAR
                     TVSREYLSDHVYHAQMEPMNATADVRRDGIDIWAGTQAPTRTTLDVAKALGTTPDMVR
                     VHQQYLGGGFGRRATVEASVDAALISKAVNRPVKLILSREDDLQAGTFRPMTSQRLDV
                     GVDAAGKIVAWQHRVVGEPVGDFVYHPGYNKAAKDRDTIFMMGAELPYYNKVDHWRSE
                     HLMAPERTRVA"
     gene            complement(1560..2012)
                     /locus_tag="VIG70_13010"
     CDS             complement(1560..2012)
                     /locus_tag="VIG70_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8251518.1"
                     /translation="MIKFVVNGTEAYSEQPGDTPLLWVLRDELGLTGTKFGCGVAQCG
                     ACTVHVNGQSARACVTPVSAVAGKKVTTIEGLSPVGDHPLQKAWIAEQVPQCGYCQSG
                     QIMQAAVLLAKNKNPSREQIVTHMNGNLCRCGTYNGIVRAIQRASKEA"
     gene            complement(2009..>2689)
                     /locus_tag="VIG70_13015"
     CDS             complement(2009..>2689)
                     /locus_tag="VIG70_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY8251519.1"
                     /translation="TLPLARRKRLLELAVRHDVLIIEDDAYGDVRFDGEEVPALAALA
                     RDAGAEDRLVHLSTLSKIVAPGLRVGWMVAPPEILQRCVIAKQTVDLSTSPWIQGAAA
                     HYLKSGQLARHLPRIVAGYREQAHAMTQALRERLAGKFMFGAPQGGMFVWGFLPNGVD
                     AAALLRHAIEEKVMYVPGAPFYAEAANPQSVRLCFSMSTPERIRTGVERLGRAFQRYD
                     TISREEKP"
BASE COUNT          379 a          928 c          922 g          460 t
ORIGIN      
        1 cgcgacgcgc gtgcgctcgg gcgccatcag gtgctcggag cgccagtggt cgaccttgtt
       61 gtagtaggga agctcggcgc ccatcatgaa gatggtgtcg cggtccttcg ccgccttgtt
      121 gtaacccggg tgatagacga agtcgcccac cggctcgccg accacgcggt gctgccaggc
      181 gacgatcttg ccggccgcgt cgacgccgac gtcgagccgc tgcgaggtca tcgggcggaa
      241 agtgcccgcc tggaggtcgt cctcgcggct caggatcagc ttcaccggcc ggttcaccgc
      301 cttcgagatg agcgcagcgt cgaccgaggc ttcgacggtc gcgcgtcggc cgaagccccc
      361 accgaggtac tgctggtgca cgcgcaccat gtccggcgtg gtgccgaggg ccttcgccac
      421 gtcgagggtc gtgcgcgtgg gggcctgcgt gccggcccag atgtcgatgc cgtcgcgccg
      481 cacgtcggcg gtggcgttca tcggctccat ctgcgcgtgg tagacgtgat cgctgaggta
      541 ctcgcggctc acggtgcgcg ccgcgccgcc gatcgccttc gccgggtcgc ccttcgactt
      601 ccattcgacg ccgacgcgct tgggatcgcg gccgtcgccg aggtaagtct cgagatcctt
      661 gtcggtgttg atcgcatcgc ccggctggcc gctgcgccag gtgaccttga gctgcttgcg
      721 cgccttgctc accgccgcga aggtctcgcc aacgaccgcg acgccgtgat cgagcgtcac
      781 cacgtcgacg atgcccggct gcttcttcac gtcggcagcg ttcgacgacg cggggccgct
      841 gccgcggacg ggcgagcgca cgaaggtggc gtacgccatg ccgggaacgc gggcgtcgat
      901 gccgtatttc gccgcacccg tcgctttcgc cggcacgtcg acgcgcggca cgcccttgcc
      961 gagcagccga tagtcgcccg gttttttcag gtccttggtg gcgtcgattg ccggcggcgt
     1021 cgccggcgga gtggcgaagg acgcgatctc gccgtagctc atcttccggc cgctcggctg
     1081 gtgcacgacg gtgctcggct cggtgacgca ctcggccgcg ggaacgcccc acttcgtcgc
     1141 ggcggcgtcg acaagcacgc gccgcgcctg cgcgcccgcc atgcgcatcg gcgtccaata
     1201 gccgagcgtc gaaatgctcg ccaccacgta ctgcgccttg aagatcgggt ggtggtaggc
     1261 cggcgcgatc ggcgactgca ccaccttcac cttgctccag tcggcgtcga gctcctcggc
     1321 cacgatgagc ggcaggccgg tcgagatgcc ctgccccatc tccgccaccg gctgctggat
     1381 ggtgatcgtg ccgtcgggcg cgatggaaac gtacgcgttg agggtgcccg gtgtttgcgc
     1441 ttttgcttct ttcccaacac ctagagtaaa tgcaaaagcc aagccggcac tgccgcccat
     1501 gaaggcgcgc cggctgatct tgaactcgag cagctcgaag tcgttcatgc cgatcatggc
     1561 taggcctcct tcgaggcgcg ctggatcgcg cgcacgatgc cgttgtaggt gccgcaacgg
     1621 cacaggttgc cgttcatgtg ggtcacgatc tgctcgcgcg aggggttctt gttcttggcg
     1681 agcagcaccg ccgcctgcat gatctgcccg gactggcagt agccgcactg cggcacctgc
     1741 tcggcgatcc acgccttctg caaaggatgg tcgcccacgg gggacaggcc ttcgatcgtg
     1801 gtgactttct ttccggcgac cgcggaaacc ggcgtgacac acgcgcgcgc ggattgcccg
     1861 ttgacgtgaa ccgtgcaggc gccgcactgc gcgacgccgc agccgaactt ggtgccggtg
     1921 agcccgagct cgtcgcgcag cacccacagc agcggggtat cgccgggctg ctcggaatag
     1981 gcttccgtgc cgttgacgac aaacttgatc atggtttctc ctccctggaa atcgtatcgt
     2041 accgctgaaa cgccctgccg agccgctcca cgccggtgcg gatgcgctcg ggggtcgaca
     2101 tcgagaagca caggcgcacg ctctgcgggt tcgccgcttc tgcgtagaac ggcgcgcccg
     2161 gcacgtacat caccttctcc tcgatcgcgt ggcgcagcag cgccgcggcg tccacaccgt
     2221 tgggcaggaa cccccagacg aacatcccgc cctggggggc gccgaacatg aattttcctg
     2281 caagccgctc gcgcagcgcc tgcgtcatgg cgtgcgcctg ctcgcggtag ccggcgacga
     2341 tgcgcggcag gtggcgggcg agctggccgg atttcaggta gtgggccgcc gccccctgga
     2401 tccacggcga ggtcgacagg tcgacggtct gcttggcgat cacgcagcgc tgcaggatct
     2461 cgggcggcgc caccatccag ccgacgcgca gccccggcgc gacgatcttc gacagcgtgg
     2521 acaggtggac cagccggtcc tcggcgcccg cgtcgcgcgc gagcgcggcg agcgccggca
     2581 cctcctcgcc gtcgaagcgc acgtcgccgt aggcgtcgtc ctcgatgatg aggacgtcat
     2641 ggcgtaccgc gagctcgagc agccgcttgc gtcgcgcgag cgggagcgt
//
LOCUS       DATBSZ010000203         8625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_991210, whole genome shotgun sequence.
ACCESSION   DATBSZ010000203 DATBSZ010000000
VERSION     DATBSZ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8625
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991210"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VIG70_13020"
     CDS             <1..734
                     /locus_tag="VIG70_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY8251520.1"
                     /translation="MIQDLAIFWPALVAGLLVTATHVPLGIQVLNRGIVFIDLAVAQF
                     AGLGVILGDWLGFEHHGPAVQVFALGAALAGALLLNLTERLWPQVQEAIIGVAFVLAA
                     NAAILLLAANPHGAEHLKDLLVGQILWVKPGELPILAVVSAAILALTFLWRSRTGFYL
                     LFACAVTVSVQLVGIYLVFATLIVPALATLQLRRGRLTACYVLGALGYAAGLGLSAVT
                     DLPTGPLVVCTLTALGTALFLTRRI"
     gene            797..1333
                     /gene="hslV"
                     /locus_tag="VIG70_13025"
     CDS             797..1333
                     /gene="hslV"
                     /locus_tag="VIG70_13025"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEY8251521.1"
                     /translation="MEQFHGTTILSVRRGAAVALGGDGQVTLGQVVLKGGARKVRRLY
                     QDRILAGFAGGTADAFTLFERFESKLDKHQGNLMRSAVELAKDWRTDRVLRRLEAMLA
                     VADKERSLIITGAGDVLEPEHGILAIGSGGPYAQAAARALLENTQATPREIVDKALAI
                     AGDICIYTNQQRSIEVLE"
     gene            1357..2658
                     /gene="hslU"
                     /locus_tag="VIG70_13030"
     CDS             1357..2658
                     /gene="hslU"
                     /locus_tag="VIG70_13030"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY8251522.1"
                     /translation="MTPPEIVHELDKYIVGQGKAKRAVAIALRNRWRRQQVAEPMRTE
                     ITPKNILMIGPTGVGKTEIARRLARLADAPFIKVEATKFTEVGYVGRDVDTIVRDLVE
                     ISVKQTREQAMRRVRTRAQDAAEERILDVLVGLKPAGDPDNATRQNFRKKLREGELDD
                     REIDIEVAASQPQMEILAPPGMEELTTQIQGMLSGLGGGRRRLRRMRIREAVQIVTDE
                     EAARLVNDDELKTRALANAEQNGIVFLDEIDKITSRGELQGADVSRQGVQRDLLPLVE
                     GTTVSTRYGMVKTDHILFIASGAFHMAKPSDLIPELQGRFPIRVELDSLSVDDFERIL
                     VATDASQVKQYQSLLGTEGVTLDFRPGAIRRIAELAFEVNEKTENIGARRLYTVLEKL
                     LEEISFEAGKRGTGTVTIDSAYVDARLAELAKNEDLARFVL"
     gene            complement(2642..2821)
                     /locus_tag="VIG70_13035"
     CDS             complement(2642..2821)
                     /locus_tag="VIG70_13035"
                     /inference="COORDINATES: protein motif:HMM:NF025733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HEY8251523.1"
                     /translation="METPPQNPDRCARCGAQFRCGAKNRDAECWCAGLPPLEPVQGRL
                     CLCRPCLEAELRERT"
     gene            complement(2796..2981)
                     /locus_tag="VIG70_13040"
     CDS             complement(2796..2981)
                     /locus_tag="VIG70_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY8251524.1"
                     /translation="MLSCKEATRLVSQGLDRRLPFGRRVALRVHLAICDGCTNFKKQT
                     EFLRRAVARLGNPAAEP"
     gene            complement(2981..3523)
                     /locus_tag="VIG70_13045"
     CDS             complement(2981..3523)
                     /locus_tag="VIG70_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8251525.1"
                     /translation="MDDFRSRVERERSYLLRYAALQLRDPHAAEDAVQETLLAALAGE
                     ATFQGRANLRTWLTGILKHKIIDAIRRASREAPSASEDEFEALFDERGHWVEMPAAWT
                     DPDASLEQKRFFAALETCLQRLPQKISQAFMMREHLGLDTGEICKELAITPTHCWVLL
                     YRARMALRECLEKDWFGKTK"
     gene            3611..4054
                     /locus_tag="VIG70_13050"
     CDS             3611..4054
                     /locus_tag="VIG70_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY8251526.1"
                     /translation="MANVTRFDPRGDYFGELVDDLFKGFLVRPVAYDGRINGALPRMK
                     VDVAEKNGAYTVTAELAGVKKDDIQVTIDGADVTLSAEVKREKEVTQDERVLHAERTF
                     GKVSRSFSLPQEVDEAKAEAKFRDGVLELTLPKKAAAARKQVTIQ"
     gene            4081..4986
                     /gene="argB"
                     /locus_tag="VIG70_13055"
     CDS             4081..4986
                     /gene="argB"
                     /locus_tag="VIG70_13055"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY8251527.1"
                     /translation="MNAPLISSPAHSPEAAEKARILAEALPYIQQFHGKTIVVKFGGN
                     AMTDDVLKSSFARDVVLLKLVGMNPVVVHGGGPQIEQLLARLGKKGEFVQGMRVTDAE
                     TMDVVEMVLGGQVNKEVVELINQAGGKAVGLTGQDGAFIHARKMTLPPSKENGTVDLG
                     QVGEVESIDPAVIKALEAGGFIPVVAPIGTGRDGTTYNINADLVAGKLAEILRAEKLV
                     VLTNTPGVLDKNGKLLTGLTPKKIDDLVAKGVISGGMLPKIGSALDAARSGVKSVHII
                     DGRVPHAVLLEVLTDQGVGTLIRSK"
     gene            5002..5658
                     /locus_tag="VIG70_13060"
     CDS             5002..5658
                     /locus_tag="VIG70_13060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEY8251528.1"
                     /translation="MRISRLPPSRRVWIFDLDNTLHDARVHIFPSMHAQIQDFLKKQF
                     GVDDEGASRMRRDFWQRYGTTLRGLVRHHGTDPKHFLAETHVFPELADMVVRENALKH
                     ALLRLGGRKLVFSNAPRHYVEEVLRAIGLERYFDAVYTIEDTGYRGKPHAWGFHRLLR
                     KHDLDPHRCALIDDILDNLRTAHRLGMSTVWVSPVGRRVPYVDLRISSVTELPSRIRP
                     "
     gene            5658..6212
                     /gene="slmA"
                     /locus_tag="VIG70_13065"
     CDS             5658..6212
                     /gene="slmA"
                     /locus_tag="VIG70_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEY8251529.1"
                     /translation="MPRVKGERRLEILKALAGMLEAPKWERITTAALAGKLSVSEAAL
                     YRHFASKAQMYEGLIEFIENSVFTLANKITEDEADGRRQCEKLVEMLLAFAEKNPGMV
                     RVMTGDALVGEHERLQARINQFFERFESTLKQSLRVASDGDAPGRAGALLRYSLGCLH
                     QYAKSGFAKKPTESFSAQRRYLLT"
     gene            complement(6214..7443)
                     /locus_tag="VIG70_13070"
     CDS             complement(6214..7443)
                     /locus_tag="VIG70_13070"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY8251530.1"
                     /translation="MTKLRTNLRRLLLAALLLVPASSFADAVLDWNEAGLAAVLAARQ
                     SPAEGARTMTMMHVAMFNAVNAIERRYANYGFENRPGSGASAGAAAAAAARTVLVGLF
                     PDQREALEKTYAASLQKLSGERGLEPGVALGELSGRDCLAMRTKDGVGLANVYRPITT
                     AGTYIPTAVTASHDWREVKPWVMKSPSQFRPEPPPALTSATWTRDYNEIKEIGARNST
                     KRTPEQTEVARFWTATGVVTWNPIVRSLATAKPRSLVDNARLFALVNMAATDSFVAVF
                     EAKYAHNFWRPITAIRNGDIDGNDATARDPAWLPLVETPMHPEYPCAHCISSGAVGAV
                     LESEFGTGRLGPIAMTSPTAPGVTRRFERIADYVKEVDNARIWGGIHYRNSAEVGERM
                     GREIGRLVATSVMLPAH"
     gene            7559..8272
                     /locus_tag="VIG70_13075"
     CDS             7559..8272
                     /locus_tag="VIG70_13075"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8251531.1"
                     /translation="MAGFGQFCPIAVACEVFAERWTPIILRELFAGSHRFNEIHRCIP
                     LISRPLLARRLRELEAAGVIRSTPQQKGKPRDYHLTASGKEFRAAVDALGTWKQRWTI
                     RVNPENLDSSLLMWNIRRRVALERLPPRRVVVQFEFRGVPAGRSMLKKCWLILEHSGS
                     DVCLSDPGFEVDVYVDADLAAMANVWLGDLPFAEAVRQKKIKVMGVPALVREFPGWLL
                     LSHFARVPRPPAEFPAAHG"
     gene            complement(8288..>8625)
                     /locus_tag="VIG70_13080"
     CDS             complement(8288..>8625)
                     /locus_tag="VIG70_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEY8251532.1"
                     /translation="LVSGAAIRFDGQLMVFDLRSNQSACYACLFPEDAQVEEVQCSTM
                     GVFAPLTGVIGASQAMEAIKLIAGVGQSLNLQILDAKSGEWRSVRVRRDPGCSVCSKR
                     QSGTDHVFR"
BASE COUNT         1434 a         2862 c         2919 g         1410 t
ORIGIN      
        1 cgatgatcca ggacctcgcg atcttctggc cggcgctggt cgcggggctc ctcgtcaccg
       61 cgacccacgt gccgctcggc atccaggtgc tgaatcgcgg catcgtgttc atcgatctcg
      121 cggtggcgca gtttgccggg ctcggcgtga tcctcggcga ctggctcggc ttcgagcacc
      181 acgggccggc ggtgcaggta ttcgcgctcg gcgccgcact cgccggcgcg ttgctgctca
      241 atctcaccga gcgcctctgg ccgcaagtgc aggaggcgat catcggcgtc gctttcgttc
      301 ttgccgcgaa cgccgcgatc ctcctgctcg ccgccaaccc gcatggcgcc gagcatctca
      361 aggacctcct ggtcggccag atcctctggg tgaaaccggg cgagctgccg atcctggcgg
      421 tggtctcggc ggcgatcctt gcgctcacgt tcttgtggag gagccgcacg ggcttctacc
      481 tgctgttcgc ttgcgccgtg acggtgtccg tacagctcgt cggcatctat ctcgtcttcg
      541 ccacgctgat cgtgccggcg ctcgccacgc ttcagctgcg gcgaggccgg ctgacggcgt
      601 gctacgtgct gggcgcgctg ggctatgccg cgggactcgg attgtccgcg gtgacggatc
      661 tgccgaccgg cccgctcgtg gtgtgcacgc tgaccgcgct gggcaccgcg ctgttcctga
      721 cccgccgaat ctgattagta ttgcaatccg gccgcgccgg cctcatcttt gccttccgtt
      781 tcccgaattt cacctcatgg aacagttcca cggcaccacg attctttccg ttcgacgcgg
      841 cgcagccgtc gcgctcggcg gcgacggcca ggtgacgctc ggccaggtcg tgctgaaggg
      901 cggcgcgcgc aaggtgcgcc ggctctacca ggaccgcatc ctcgccggct tcgccggcgg
      961 caccgccgac gccttcacgc tgttcgagcg cttcgagtcc aagctcgaca agcaccaggg
     1021 gaacctgatg cgctcggcgg tcgagctggc gaaggactgg cgtaccgacc gcgtgctgcg
     1081 gcgcctggag gcgatgctcg cggtcgccga caaggagcgc tcgctcatca tcaccggcgc
     1141 gggcgacgtg ctcgagcccg agcacgggat tctggcgatc ggctctggcg ggccgtatgc
     1201 gcaggcggca gcgcgggctt tgctcgagaa cacccaagcc acccctcgag aaatcgtgga
     1261 caaggcgctg gcgatcgcgg gcgacatctg catctacacg aaccagcagc gcagcatcga
     1321 ggtgctggaa tgaagccaaa cgacggcata caaggcatga cgcctcccga gattgtccac
     1381 gagctcgaca agtacatcgt cgggcagggg aaggcgaagc gtgcggtcgc gatcgcgctg
     1441 cgcaaccgct ggcggcgcca gcaggtcgcg gagccgatgc gcaccgagat cacgcccaag
     1501 aacatcctga tgatcgggcc gaccggcgtc ggcaagaccg agatcgcgcg ccgcctggca
     1561 cgcctcgccg acgcgccgtt catcaaggtg gaggcgacca agttcaccga ggtgggctac
     1621 gtcgggcgcg acgtcgacac catcgtgcgc gatctggtcg agatcagcgt caagcagacg
     1681 cgcgagcagg cgatgcgccg cgtgcgcacg cgcgcgcagg acgccgccga ggagaggatt
     1741 ctcgacgtcc tcgtcggcct caagcccgcg ggcgacccgg acaatgccac gcgccagaat
     1801 ttccgcaaga agctccgcga gggcgagctc gacgatcgcg agatcgacat cgaggtcgcg
     1861 gcgagccagc cgcagatgga gatccttgcg cccccgggca tggaggagct caccacgcag
     1921 atccagggca tgctctcggg cctgggcggc ggccggcggc gcctgcggcg catgaggatc
     1981 cgcgaggccg tgcagatcgt caccgacgag gaggcggcgc gcctggtgaa cgacgacgag
     2041 ctgaagacgc gcgcgctggc caacgccgag cagaacggca tcgtgttcct cgacgagatc
     2101 gacaagatca ccagccgcgg cgagctgcag ggcgccgacg tctcgcgcca gggcgttcag
     2161 agggatctcc tgccgctggt cgaaggcacg accgtctcga cgcgctacgg catggtgaag
     2221 accgatcaca ttttgttcat cgcctcgggt gcgttccaca tggccaagcc ctccgacctg
     2281 atcccggagc tgcaggggcg tttcccgatc cgcgtcgagc tcgactcgct ctcggtcgac
     2341 gacttcgagc gcatcctggt tgcgaccgac gcgagccagg tcaagcagta ccagtcgctg
     2401 ctgggcaccg agggcgtgac cctcgacttc cgccccgggg cgatccggcg catcgccgag
     2461 ctcgccttcg aggtgaacga gaagaccgag aacatcggcg cgcggcggct ctacaccgtg
     2521 ctcgagaagc tgctcgagga gatttcgttc gaggccggca agcgcggcac ggggacggtg
     2581 acgatcgact ccgcctatgt cgatgcgcgc ctcgcggagc tcgcgaagaa cgaggacctg
     2641 gctaggttcg ttctctaagc tccgcttcca ggcaagggcg gcagaggcac aagcgcccct
     2701 gcaccggctc gagcggcggc agccccgcgc accagcactc ggcatcgcgg ttcttcgcac
     2761 cgcagcggaa ctgcgccccg cagcgggcgc aacggtcagg gttctgcggc ggggtttcca
     2821 aggcgggcta cggccctgcg gagaaactcg gtctgcttct tgaaattggt gcagccgtcg
     2881 cagatcgcca ggtgcacgcg cagcgcgacg cgccggccga agggcagccg gcggtcgagg
     2941 ccttgggaca cgaggcgcgt tgcctctttg cacgacagca tcatttagtc tttccgaacc
     3001 agtccttttc caggcattcg cgcagcgcca tgcgcgcgcg atagagcagc acccagcagt
     3061 gcgtcggggt gatggcgagt tccttacaga tctcgccggt gtcgagcccc aggtgctcgc
     3121 gcatcatgaa tgcctgcgag atcttttgcg gcaggcgctg caggcacgtc tcgagcgcgg
     3181 cgaaaaagcg cttctgctcg agcgaggcgt cggggtcggt ccaggcggcg ggcatttcca
     3241 cccagtggcc gcgctcgtcg aacaaagcct cgaattcgtc ttcggaagcc gaaggcgctt
     3301 cgcggctcgc ccggcggatg gcgtcgatga tcttgtgctt gaggatcccg gtgagccagg
     3361 tgcgcaggtt cgcgcgcccc tggaacgtgg cctcgcccgc gagcgccgcg agcagagtct
     3421 cctgcaccgc gtcctcggcg gcgtgcgggt cgcgcagctg cagggcggca taccgcagca
     3481 ggtagctgcg ctcgcgttcg acgcgggaac gaaagtcgtc cataggggcg attttcgatt
     3541 ttacaagggg cttgaaaccc cgctggcaga ccctaccttc ccggtactgc ctcaggaaaa
     3601 ggagacgcaa atggcaaacg taactcggtt cgatccgcgc ggtgactact tcggcgaact
     3661 ggtcgatgac ctgttcaaag gctttctggt gcgcccggtt gcctacgacg gccgtatcaa
     3721 tggcgcgctg ccgcgcatga aggtggacgt cgccgagaag aacggcgcct acaccgtcac
     3781 cgccgagctg gccggggtga agaaggacga catccaggtc accatcgacg gcgccgacgt
     3841 caccctcagc gccgaagtga agcgcgagaa ggaagtgacg caggacgagc gcgtgctgca
     3901 tgccgagcgc acgttcggca aggtgtcgcg cagcttcagc ctgccgcagg aagtggacga
     3961 ggccaaggcc gaagccaagt ttcgcgacgg cgtgctcgag ctgacgctgc ccaagaaagc
     4021 cgcagccgcg cgcaagcaag tcacgatcca gtaggttttt cggtagtatc ggggggcgcg
     4081 atgaacgccc ccctcatttc ttctcccgct cattctccgg aagctgcgga gaaggcgcgc
     4141 atcctcgccg aggcgctgcc ctacatccag cagttccacg gcaagaccat cgtcgtcaag
     4201 ttcggcggca acgccatgac cgacgacgtc ctgaagagct ccttcgcgcg cgacgtggtg
     4261 ctgctcaagc tcgtcggcat gaacccggtg gtggtgcatg gcggcgggcc gcagatcgag
     4321 cagctgctcg ccaggctggg caagaagggc gagttcgtcc agggaatgcg cgtcaccgac
     4381 gccgagacca tggacgtggt ggaaatggtg ctcggcggcc aggtgaacaa ggaggtcgtc
     4441 gagctcatca accaggcggg cggcaaggca gtcgggctca ccggccagga cggcgccttc
     4501 atccacgcgc gcaagatgac gctgccgcct tccaaggaga acggcacggt cgacctcggc
     4561 caggtcggcg aggtcgaatc gatcgacccg gcggtgatca aggccctgga agccggcggt
     4621 ttcatcccgg tggtcgcgcc gatcggcacc ggccgcgacg gcaccaccta caacatcaac
     4681 gccgacctcg tcgccggcaa gctcgccgag atcctgcgcg ccgagaagct ggtggtcctc
     4741 accaataccc cgggcgtcct cgacaagaac ggcaagctcc tcaccggcct cacgcccaag
     4801 aaaatcgacg acctggtggc gaagggtgtg atctcgggcg gcatgctgcc gaaaatcggc
     4861 tcggcgctcg acgccgcgcg cagcggcgtg aagagcgtgc acatcatcga cgggcgtgtt
     4921 ccccatgccg tcctgctgga agtcctcacc gatcagggcg tcggcaccct catcagaagc
     4981 aaatagcctg ggaagctagg cgtgcgtatc tcgaggttgc cgccctcgcg gcgggtgtgg
     5041 atcttcgacc tcgacaacac cctgcacgac gcgcgggtgc atatttttcc gtcgatgcac
     5101 gcccagatcc aggattttct gaaaaagcag ttcggcgtcg acgacgaggg cgcgagccgc
     5161 atgcgccgcg acttctggca gcgctacggc accacgctca gggggctggt gcgccaccat
     5221 ggcaccgacc cgaagcattt cctggccgaa acccacgtct tccccgagct cgccgacatg
     5281 gtggttcgcg agaacgccct caagcacgcc ctgctgcggc tcggcgggag gaagctcgtg
     5341 ttctccaacg cgccgcggca ctacgtcgag gaggtgctgc gcgccatcgg cctcgagcgc
     5401 tatttcgacg ccgtttacac gatcgaggac acggggtacc gcggcaagcc gcacgcgtgg
     5461 ggcttccacc gtctgttgcg caagcacgac ctcgacccgc accgctgcgc gctcatcgac
     5521 gacatcctcg acaacctgcg caccgcgcat cgccttggca tgtcaacggt ttgggtaagc
     5581 cccgtcggcc ggcgcgtacc ctacgtggac cttcgaattt cctcggtgac cgaactgcca
     5641 agtagaatcc ggccttgatg ccgcgggtca aaggcgaacg cagactcgag atcctcaagg
     5701 ccctggcagg gatgctggag gcgccgaagt gggagcgcat cacgaccgcc gcgctcgccg
     5761 ggaagctcag cgtctccgag gccgcgctct atcgccactt cgcctcgaaa gcccagatgt
     5821 acgaaggcct gatcgagttc atcgagaact cggtgttcac gctggccaac aagatcaccg
     5881 aggacgaggc cgacggccgc cggcaatgcg agaagctcgt cgagatgctg ctcgccttcg
     5941 ccgagaagaa cccgggcatg gtgcgcgtga tgaccggcga tgcgctggtg ggcgagcacg
     6001 agcgcctgca ggcgcgcatc aaccagtttt tcgagcgctt cgagtcgacc ctcaagcagt
     6061 cgctgcgcgt cgcgtccgac ggcgacgcgc cgggccgcgc cggcgcgctg ctgcgctact
     6121 cgctcggctg ccttcaccag tacgcgaaga gcggcttcgc gaaaaaacct accgaaagct
     6181 tctcagcgca aagacgttat ctattgacct gacctagtgg gcggggagca tgacgctcgt
     6241 ggccacgagg cggccgatct cgcgtcccat gcgctcgccg acttcggccg agttgcggta
     6301 gtggatgccg ccccagatgc gggcgttgtc gacttccttc acgtagtcgg cgatccgctc
     6361 gaagcggcgc gtgacgccgg gcgcggtcgg gctggtcatg gctatcgggc cgaggcggcc
     6421 ggtgccgaac tcggattcga gcaccgcgcc cactgccccg gagctgatgc aatgcgcgca
     6481 ggggtactcg ggatgcatcg gcgtctccac gagcggcagc catgccggat cgcgtgcggt
     6541 ggcgtcgttg ccgtcgatgt cgccgttgcg gatcgcggtg atcgggcgcc agaagttgtg
     6601 cgcgtacttg gcctcgaaca ctgcgacgaa agagtcggtg gcggccatgt tgacgagcgc
     6661 gaagaggcga gcgttgtcga ccaggctgcg cggcttcgcc gtcgcgagcg agcgcacgat
     6721 cgggttccag gtcaccacgc cggtcgcggt ccagaagcgc gcgacttcgg tctgctcggg
     6781 tgtgcgcttc gtgctgttgc gcgcgccgat ttccttgatc tcgttgtagt cccgcgtcca
     6841 ggtggcgctc gtcagcgccg gcggcggctc gggccggaac tgcgacgggc ttttcatcac
     6901 ccagggcttc acctcgcgcc agtcgtggct cgcggtgacg gccgtcggga tgtaggtccc
     6961 ggccgtggtg atgggacggt agacgttcgc caggccgacg ccgtccttgg tgcgcatggc
     7021 gaggcagtcc ctgccggaga gctcgccgag cgcgacgccg ggctcgaggc cgcgctcgcc
     7081 agagagcttc tgcagcgacg ccgcgtaggt cttctccagc gcttcgcgct ggtcggggaa
     7141 gaggccgacg agcacggtac gcgccgcggc agcggctgcc gcgcctgccg acgcgccgga
     7201 gccggggcgg ttctcgaagc cgtagttcgc atagcggcgc tcgatcgcgt tgaccgcgtt
     7261 gaacatcgcg acgtgcatca tcgtcatcgt ccgcgcgccc tcggccgggc tctggcgcgc
     7321 ggcgaggacg gcggcgaggc ccgcctcgtt ccagtcgagg actgcatcgg cgaaggagct
     7381 cgccggcacg agcagcaggg cggcgagaag caggcggcgc aggttggtcc gcagtttggt
     7441 catggggcct cccgagggtg gtctaaaggg ccccgccaag atgcccgacc gcgcgcggcc
     7501 gcgactagtg tagaaaatga acttgtcccg ggccgggcgc gccgctaatg tttgcgccat
     7561 ggcgggattc ggccagttct gcccgatcgc ggtggcgtgc gaggtcttcg cggagcgctg
     7621 gacgccgatc atcctgcgcg agctgttcgc gggcagccac cgcttcaacg agatccaccg
     7681 ctgcatcccg ctcatctcgc gcccgctgct cgcgcgccgg ctgcgcgagc tcgaggcggc
     7741 gggggtgatc cgcagcacgc cgcagcagaa gggaaagccg cgcgattatc accttaccgc
     7801 gtcgggcaag gaattccgcg ccgccgtcga tgcgctcggc acctggaaac agcgctggac
     7861 gatccgcgtg aatcccgaga acctcgactc aagcctcctg atgtggaaca tccgccgccg
     7921 cgtggcgctc gagcgcctgc cgccgaggcg cgtcgtggtc caattcgagt tccgcggcgt
     7981 tcctgccggc cgcagcatgc tgaagaagtg ctggctgatc ctcgagcact cgggctccga
     8041 cgtctgcctc agcgatcccg gcttcgaggt cgatgtctac gtcgacgccg atctcgccgc
     8101 gatggcgaat gtctggctcg gcgatctgcc gttcgcggag gcggtgcggc agaagaagat
     8161 caaggtgatg ggagtgcccg cgctggtgcg cgagttcccg ggctggctgc tgctcagcca
     8221 tttcgccagg gtgccgcgcc cgccggcgga atttccggcg gcacacggct gagagtcttc
     8281 ctagcgacta ccggaaaacg tggtctgtcc ctgattgtct cttgctgcag acgctgcatc
     8341 cgggatccct tctcacccgc acgctgcgcc actccccgct ctttgcgtcc aggatctgga
     8401 gattcaatga ttggccaacg ccggcgatga gcttgatcgc ttccatcgcc tggctcgcac
     8461 cgatcacgcc ggtaaggggt gcaaagacgc ccatggtcga gcactggact tcctcgactt
     8521 gtgcgtcctc gggaaacagg cacgcgtagc aagccgattg gttgcttctc agatcgaaga
     8581 ccatcaactg gccgtcgaag cggatcgccg cgccggagac gagcg
//
LOCUS       DATBSZ010000204        14416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2289273, whole genome shotgun sequence.
ACCESSION   DATBSZ010000204 DATBSZ010000000
VERSION     DATBSZ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14416
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2289273"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VIG70_13085"
     CDS             <1..424
                     /locus_tag="VIG70_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8251533.1"
                     /translation="RVVVERSNPAAVSRELWKGLVRFNREHAGPLSYARTVMSLRDGK
                     GRLLGGLILQSYWRGSYIELLWVSARTRGAGFGRRLLKEAEKRARRRGSRLIHLNTYS
                     FQAPGFYEKQGYRRFGEVSGSPQGERRHFYVKRLRAAR"
     gene            454..1128
                     /locus_tag="VIG70_13090"
     CDS             454..1128
                     /locus_tag="VIG70_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251534.1"
                     /translation="MRLAGAGDPVQEARELLAKDEEERAEPSEEEDRKRSALAADLVA
                     LHPSLRVEPSDNGFSYGCSFETDDPECPVPYIYVRIGTGSVSFSYSADPKVAFREAFR
                     AIAVFERHGYAAYDPQTESMLSSSQSAEPGESSFRATRDAVVQQMRDRGEIVLGYPAP
                     KKGSLLVPGLIVFVIVAIALVLIRQHYRVERSPETVKALRDAQDRLHPPRPAPSDRRE
                     TEKPSR"
     gene            1187..1792
                     /locus_tag="VIG70_13095"
     CDS             1187..1792
                     /locus_tag="VIG70_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251535.1"
                     /translation="MKYGRSLLVVALVVAAGSAQAAGIGVRAGTTGLGADFGWGIAPT
                     LGGRIGLSGMNFNTDVETSDVNYDAKVKLANLNLFLDWSPLGPFRLTAGFIANNNKVD
                     LTGQGSGSLSGTTLTGTVKPDKDFAPYLGVGYGNVWTKGVNFYFDLGVMFQGSPQVSL
                     SCQPAGSPQCAQVGAEQQRVQDELNKYKYYPVLNLGITIGF"
     gene            1796..2068
                     /locus_tag="VIG70_13100"
     CDS             1796..2068
                     /locus_tag="VIG70_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEY8251536.1"
                     /translation="MCRSIKTLFNFKPPATGEEVRAASLQFVRKISGYHKPSHTNEAA
                     FDAAVEQVAAIAHKLLESLQTSAEPRDREVEAARARARAAERFGTP"
     gene            2065..2406
                     /locus_tag="VIG70_13105"
     CDS             2065..2406
                     /locus_tag="VIG70_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251537.1"
                     /translation="MITGTHIVITSKKPEADHAFLRDVLKLPHVNEGGYLIFALPPAE
                     VSVHEGKKDSVHEMFLLCDDVKTFVADMKKRRIVCGPVKDQGWGLLTQMTLPGGGKLG
                     VYQPRHARPKM"
     gene            2409..2975
                     /locus_tag="VIG70_13110"
     CDS             2409..2975
                     /locus_tag="VIG70_13110"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8251538.1"
                     /translation="MQAGGNVFVCAHGAGGHKDDRGMVAAAAALGDAGLDVLRFNFAY
                     RERGSKLPDPMPELKAEVAAVASRAREELKPRKLILGGRSMGGRAASMLAADGFECDG
                     LLLLAYPLHPAGKPEKLRDAHLPKIRVPVLCFNGTRDELCRRDLMEKALEGLSWQMHW
                     LDGAGHSFRAKDFEAIAEVSRAWLSRLG"
     gene            complement(2944..3879)
                     /gene="senB"
                     /locus_tag="VIG70_13115"
     CDS             complement(2944..3879)
                     /gene="senB"
                     /locus_tag="VIG70_13115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HEY8251539.1"
                     /translation="MKIALVTPAGPGMRNGNRHTALRWAAFLRAAGHVVSVSTEETSS
                     GADLMLALHARRSHASIRSFAIGKPLVVALTGTDIYRDMRTSKEARESLELAHRLVVL
                     QPKAAEELPARLRKKVRVVVQSSATLLRHRPVAKSFRVCVIGHLREEKDPLRTLSSLK
                     FIPEALPLEVVHLGAALDRELVPRTNDPRYRWLGSVPHARALRWLASSHVMVISSRME
                     GGANVVCEALRIGVPVLASRIPGNVGLLGAGYRGYFPVEDQRALARLMTRASVDRAFY
                     GTLKSQIAKLRPMVAPRAEARALLAVIRDAIARRG"
     gene            3897..4910
                     /locus_tag="VIG70_13120"
     CDS             3897..4910
                     /locus_tag="VIG70_13120"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY8251540.1"
                     /translation="MQELSLRLKKAREHTRRVTDDLAGEREFGPQLAIVNPPRWEVGH
                     VGWFQEFWCLRGGSEERASILPNADALYNSATVAHDARWGLPLPSFADTVAYRDEVLE
                     RVVRGLEDIDPYFVELALRHELMHAEAFHYTRQTLGYPGPEADARLTENKGDFACPGG
                     VFRLGAKPGEGFAFDNEKWSHPVVLEPFRIARRPVTYSEYRKFAQPPYCKDGMVRRFD
                     RWIPIPEDEPVRHVSWHQANAYCAFAGRRLPTEAEWECAALSGLENVGHVWEWTSSTF
                     LPYHGFVRDPYKEYSEPWFGTHKVLRGASFATPAGVSHARFRNFYTPERSDMFVGFRT
                     CAP"
     gene            4907..5689
                     /locus_tag="VIG70_13125"
     CDS             4907..5689
                     /locus_tag="VIG70_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY8251541.1"
                     /translation="MSALTSRDNPKVKHWAMLAKDARYRRTERRALIEGPHLLAAALQ
                     HGYRPVAVLATEKAAAGEEIGGLIAQCGVRAVMLAESLFGAISDVETPQGIAAEIAIP
                     DEAEGEGLDTVFLEGVQDPSNVGAIIRSAAGFGIGRVLLDRDCADAWSPKALRAGMGG
                     HFALRVYETGRLAAEMDAFKGTLACAVARGGVPLREAALSGSMGWIFGAEGRGLSEET
                     LRKVGLQVTIPMKPGTESLNVAAAAAICFYEAFSRSNSGTEP"
     gene            complement(5760..6578)
                     /locus_tag="VIG70_13130"
     CDS             complement(5760..6578)
                     /locus_tag="VIG70_13130"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEY8251542.1"
                     /translation="MAQRRTVFFVSDGTGITAQMLGHSLLTQFEGVEFSQVTLPFVDS
                     TEKAEDCIARIAAEAGNGQAIVFSTLVNNDVRAVVRRANALFIDFFESFIDPLEAGLG
                     VKSSHTIGRSHSAMDKKEYQQRIEAINFAMAHDDGASHRELSLADVILVGVSRSGKTP
                     TSLYLAMQFGVKAANYPLIPEDFHRMKLPEALRGEKSRLYGLTIASDRLHEIRKERRP
                     GSRYADLDNCRFEVEEAENLMRREGIRSINSTSKSIEEIATTILRELRIKRQIY"
     gene            6598..8976
                     /gene="ppsA"
                     /locus_tag="VIG70_13135"
     CDS             6598..8976
                     /gene="ppsA"
                     /locus_tag="VIG70_13135"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEY8251543.1"
                     /translation="MRVAWLKDLRMADLEQVGGKNASLGEMIGGLSAAGIRVPGGFAT
                     TADAFREFMGELAPRIDERLKKLDPKDVDALAKCGAEVRSWILARPFPAELEREIRSK
                     YQDLTSQTSPETSFAVRSSATAEDLPEASFAGQQETFLNVRGADNVLDAIRHVFASLY
                     NDRAISYRAHHNFDHAAVALSAAVQQMVRSDLGASGVLFTLDTESGFRDVVFITSSYG
                     LGELLVQGAINPDEFYVHKPMLEKGRPAIVRRGLGSKLQKMVFAKSDKAGKSTRVVDV
                     AEKERHSFSLSDKEILELARYAVAIERHYGRPMDIEWAKDGTDGRLYVLQARPETVKS
                     RTRDVEERYALKGSSRVMASGRAIGHKIGSGAVRIVPDASQMSRVKKGDVLVTDMTDP
                     NWEPVMKLAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATRRLKDGQPVTVSCAEG
                     DTGSVYEGLLPFEVSTRERGEMPRIPVKIAMNVGNPQLAFEFAQIPNAGVGLARLEFI
                     INNEIGVHPRACLDYEELPKPLKAKLDEKTRGYADARSFYRDKLVEGVATIAAAFWPK
                     PVIVRLSDFKSNEYKKLLGGERYEPDEENPMLGLRGASRYLSPDFRACFELECAAMRT
                     VRDEIGLTNVELMVPFVRTVGEAREVVSLLEKNNLQRGKNGLRIVMMCELPSNAILAE
                     EFLELFDGFSIGSNDLTQLTLGLDRDSGLVAGSFDERDAAVKALLAMAIRACRKHGKY
                     VGICGQGPSDHPDLARWLMEQGIDSLSLNPDTVVSTWMALAEAPAAKKSAAA"
     gene            complement(8962..9405)
                     /gene="smpB"
                     /locus_tag="VIG70_13140"
     CDS             complement(8962..9405)
                     /gene="smpB"
                     /locus_tag="VIG70_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY8251544.1"
                     /translation="MSIVENRKAFHNYFIEERYEAGLALEGWEVKAIRGGRATIAEGY
                     VMVRGAELYLIGVNISPLPTVSTHFTPDPTRTRKLLLRAEEIRRLIGKVEQRGYTLVP
                     LNLHYSKGRIKLEVGLGRGKLKHDKRASEREKEWNRDKQRLLRSR"
     gene            9504..9938
                     /locus_tag="VIG70_13145"
     CDS             9504..9938
                     /locus_tag="VIG70_13145"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEY8251545.1"
                     /translation="MKQIARSAIVEHSAAEMYALVEDIEAYPGFLPWCSAAVVHERSA
                     GTTKATLTVGLPALQQSFTTLNENRPGEAIDMRLVEGPFRRFGAQWRFVPLSPQACRI
                     EFSLQYEFSRRTLGRLLAPLFDGIADSMVDAFVRRAAQVYEG"
     gene            9928..10182
                     /locus_tag="VIG70_13150"
     CDS             9928..10182
                     /locus_tag="VIG70_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEY8251546.1"
                     /translation="MKVEVVYARPEGAQVVRLELPAGARLRDAVSACGFKIEDHAVGI
                     FGKRVALDHPLADGDRVEIYRPLAMDPKEARRRRASKTKR"
     gene            complement(10187..10645)
                     /locus_tag="VIG70_13155"
     CDS             complement(10187..10645)
                     /locus_tag="VIG70_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY8251547.1"
                     /translation="MKKLLVLSFFFAAAVSAQQYKWVDKDGKVRYGDVPPPGVTATPM
                     RAPPPGSASPAAPAAAAKKDGDKPLSPEAAFRKRQEEAEKDREKQAKADQDAQEKRAN
                     CARAQDALRSLESGQRIARTDSKGDRYFLDDAQTAQETARARQSVQEWCK"
     gene            10717..12168
                     /gene="guaB"
                     /locus_tag="VIG70_13160"
     CDS             10717..12168
                     /gene="guaB"
                     /locus_tag="VIG70_13160"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY8251548.1"
                     /translation="MRVIQKALTFDDVLLVPAHSRVLPREASLKTRLTRKLQLNLPLV
                     SAAMDTVTEARLAIALAQEGGIGIVHKNLPPKAQAAEVAKVKRFESGVLRDPLTVPPE
                     MPVRDVIALQRERKISGFPVVKNGRVVGIVTNRDLRFETQLDQPVKAIMTPQKKLVTV
                     REGASRQEAKELMHRHRLERVLVVGEDFELKGLVTVKDITKESEHPNACKDAQGKLRV
                     GAAVGVGEGTEERVAALVEAGADVLVVDTAHGHAQGVLDRVAWVKKNFKIEVIGGNVG
                     TGDGARALVDHGADGVKVGIGPGSICTTRIVAGVGVPQITAIQDVAEALKDVPLIADG
                     GVRYSGDIAKALAAGAHAVMLGSLFAGTDESPGEIELYQGRSYKSYRGMGSLGAMQKG
                     AADRYFQDSEMNVDKLVPEGVEGRVPYKGSAIWVIEQLMGGVRASMGYTGCSTIEELR
                     TRTRFVEITAAGIRESHVHDVQITKEAPNYRAE"
     gene            12172..13734
                     /gene="guaA"
                     /locus_tag="VIG70_13165"
     CDS             12172..13734
                     /gene="guaA"
                     /locus_tag="VIG70_13165"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY8251549.1"
                     /translation="MHQKILILDFGAQYTQLIARRVREAKVYCEIHPWDVDDAFVRRF
                     GAQGIILSGSHLSAYDDTRAHAPKAVFEAGVPVLGICYGMQSMAHQLGGRVEAGTVRE
                     FGYAEVRARGHTKLLDGIEDYRTPEGHGMLKVWMSHGDKVAELPPGFRLMASTEACPI
                     AGMADEARRFYGVQFHPEVTHTRQGTKLLHRFVRDICGCRGDWQMPEYVSEAVAKIRS
                     LVGKDEVILGLSGGVDSSVAAALIHRAIGPQLTCVFVDHGLLRLDEARQVMDTFAKHM
                     HVKVVHVDASAQFLSALKGVADPEEKRKIIGRLFVEVFQAEAKKIRNAKWLAQGTIYP
                     DVIESAGASTKKAATIKSHHNVGGLPETLHLKLLEPLRELFKDEVRELGLALGLPREM
                     VFRHPFPGPGLGVRILGEVEKEHVRLLQRADDIFIQELRSSGWYDKTAQAFAVFLPVR
                     SVGVMGDGRTYENVVALRAVQTSDFMTADWAPLPHELLARVSNRIINEVRGINRVTYD
                     ISSKPPATIEWE"
     gene            13783..13941
                     /locus_tag="VIG70_13170"
     CDS             13783..13941
                     /locus_tag="VIG70_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251550.1"
                     /translation="MVVELPELLPLELPMPLLEPVLGNWVVLGEEVELPLLLPPLAAP
                     ALEPDLSK"
BASE COUNT         2496 a         4651 c         4896 g         2373 t
ORIGIN      
        1 ccgggtcgtt gtcgagcggt cgaatccggc cgcggtttcg cgcgagttgt ggaaagggct
       61 ggtgaggttc aaccgcgagc acgcggggcc gctctcctat gctcgcacag tgatgagcct
      121 ccgggacggc aagggacgac tgctgggcgg gctgatcctg cagtcgtact ggcgggggag
      181 ctacatagag ctcctctggg tgtcagcacg aacgcgcggc gcaggattcg gcagaagact
      241 cctcaaggaa gcggagaagc gcgcgcgacg gcgcggcagc cgcctgatcc acttgaatac
      301 gtactctttt caagcgcccg gcttttacga aaagcagggc tatcggcgct tcggcgaagt
      361 gtctggttct ccgcagggtg aacgccggca cttttacgtg aagcgtctgc gtgccgctag
      421 atagaggatg ccctgtcgta cgacatatac ctggtgaggt tggccggcgc gggcgatccg
      481 gtgcaggaag caagggagtt gcttgccaag gacgaggaag agcgcgcgga acccagcgag
      541 gaagaagaca ggaagcgcag cgcgctggcc gcggatctcg tcgcactgca cccttccttg
      601 cgcgtcgagc cctccgacaa cgggttttcc tatggctgct cgttcgagac ggatgatccg
      661 gaatgtccgg tgccgtatat ctatgtccga atcggtactg gttcggtgag tttctcctat
      721 tcggcggatc ccaaggtggc gttccgcgag gccttcaggg cgatcgccgt ctttgagcgc
      781 cacggctatg cggcctacga cccgcaaacg gagtccatgc tgagctcgtc gcagagtgcg
      841 gagcctggcg agtcgtcgtt tcgtgcaacc cgggacgctg tcgtgcagca gatgcgggat
      901 cgtggcgaga tcgtgctggg ctatcccgca cccaagaaag gttccttgct cgtccccggc
      961 ctgattgtgt tcgtgatcgt agccatcgcc ctggtcctca tccggcagca ttacagggtc
     1021 gagaggtcgc cggagacggt caaggcgctc cgagacgcgc aagaccgctt gcatccgccg
     1081 agaccggcgc cttccgatcg gcgagagacc gaaaagccaa gcaggtgatg gacggcgcag
     1141 ggatgttctg atatccatgc cgcaggggac aaatctggag atcgcaatga aatacggcag
     1201 atcgcttctt gtagtggcgc ttgtagttgc ggcgggctcg gcgcaggcgg ccggaatcgg
     1261 cgtgcgcgcc ggcacgacgg gattgggcgc ggacttcggc tggggcattg cgcccacgct
     1321 cggcgggcgc atcggcctgt cggggatgaa cttcaacacc gacgtcgaga cgagcgacgt
     1381 caactacgac gcgaaggtga agctcgcgaa cctgaatctt ttcctcgact ggagcccgct
     1441 cgggccgttc cggcttacgg ccggcttcat cgccaacaac aacaaggtcg acctcactgg
     1501 ccagggctcc ggcagtcttt cgggcaccac cctgaccggc acggtaaagc ccgacaagga
     1561 cttcgcgccg tacctcggcg tcggctatgg caatgtctgg accaagggcg tcaacttcta
     1621 cttcgacctc ggcgtgatgt tccagggatc gccccaggtg agcctgagct gccagccggc
     1681 gggttctccg caatgcgctc aggtcggagc cgagcagcag agggtccagg acgagctgaa
     1741 caagtacaag tattaccccg tgctgaacct ggggatcacc atcggattct gagccatgtg
     1801 ccgaagcatc aagacgctgt tcaacttcaa gccgcccgcg accggcgagg aagtccgggc
     1861 ggcgtcgctg cagttcgtgc gcaagatttc gggctaccac aagccgtcgc acaccaacga
     1921 agcggcgttc gatgccgcgg tggagcaggt agccgcgatc gcgcacaagt tgctcgagtc
     1981 tctgcagacc agcgccgagc cgcgcgaccg cgaggtcgaa gccgcgcgcg cccgggcccg
     2041 agccgccgaa aggttcggca cgccatgatc accggcacgc acatcgtgat caccagcaag
     2101 aagcccgaag cggaccacgc gttcctgcgc gacgtcctga agctgccgca cgtcaacgag
     2161 ggcgggtacc tgatcttcgc cctgccgccg gcggaagtgt cggtgcatga ggggaagaaa
     2221 gacagcgtgc acgagatgtt tctgctgtgc gacgacgtga agacgttcgt cgccgacatg
     2281 aagaagcgcc gcatcgtctg cggcccggtc aaggaccagg gctggggcct gctcacgcag
     2341 atgaccttgc ccggcggcgg caagctcggc gtctatcaac cgcggcacgc gcggccgaag
     2401 atgtagcggt gcaggcaggc ggcaacgtgt tcgtctgcgc gcacggcgcc ggcgggcaca
     2461 aggacgaccg gggcatggtc gcggctgccg cggcgctcgg cgacgccggt cttgatgtcc
     2521 tgcgattcaa ctttgcgtat cgcgaaagag gctcgaagct tcccgatccg atgccggaac
     2581 tgaaggcgga ggtcgccgcg gtcgcctcgc gcgcccgcga ggagctcaag cctcggaagc
     2641 tcatcctcgg cggacgctcc atgggcggtc gcgccgcgtc gatgctcgcc gcggacggct
     2701 tcgaatgcga cggcctgctg ctcctcgcct atccgctgca tccggcgggg aagcccgaga
     2761 agctgcgcga cgcgcacctt cccaagatcc gcgtgccggt gctctgcttc aacggcacgc
     2821 gcgacgagct ttgccggcgc gacctgatgg agaaagcact ggaaggactt tcctggcaga
     2881 tgcactggtt ggacggcgcc gggcattcgt tccgcgccaa agatttcgag gcgatcgccg
     2941 aggtcagccg cgcctggcta tcgcgtctcg gatgaccgcg aggagggcgc gcgcctcggc
     3001 gcggggagcg accattggcc gcagctttgc gatctggctc ttcaaggttc cgtagaaggc
     3061 gcgatcgacc gaagcgcggg tcatcaaacg ggcgagggca cgctggtcct cgacgggaaa
     3121 ataacctcga tagccggcgc ccagcagccc gacgttgccc gggatgcgcg aggcgaggac
     3181 gggaactccg atgcgcaggg cctcgcacac cacgttcgcg ccgccctcca tgcgcgagct
     3241 gatcaccatg acgtggctgg aggcgagcca gcgcagcgcc cgcgcatgcg gcacgctgcc
     3301 gagccagcgg tagcgcgggt cgttggttct gggaaccagc tcgcgatcga gcgctgcgcc
     3361 cagatggaca acttcgaggg gcaacgcctc cggaatgaac ttgagggaag agagtgtccg
     3421 cagcgggtcc ttttcctcgc gcagatggcc gatcacgcaa acccgaaaag acttcgcaac
     3481 cggccggtgg cgcagcagcg tggccgagct ctgcacgacc acccggactt tcttccgcag
     3541 cctcgccggc agctcttcgg cggccttcgg ctgcagcacg accagccggt gcgcaagctc
     3601 gagcgattcg cgcgcctcct ttgaggtccg catgtcgcgg tagatgtccg tgccggtcaa
     3661 cgcgaccacg aggggctttc cgatcgcaaa gctgcggatc gaggcgtggc tgcgccgggc
     3721 gtgcagcgcg agcatcaggt cggcgccgga agaggtctct tccgtggaaa ccgacactac
     3781 gtgccccgcg gcgcgcagga acgccgccca gcgcagcgcg gtgtggcggt tgccgttgcg
     3841 catgcctggc cccgccggcg tcaccaaggc gattttcatt cgctagactg gaaggtatgc
     3901 aagagctttc gctccggttg aaaaaagcac gagagcacac ccgccgcgtc accgacgacc
     3961 tcgcgggcga gcgcgaattc gggccgcagc ttgcgatcgt caatccgccg cgctgggaag
     4021 tcgggcatgt cggctggttc caggagttct ggtgcctgag gggtggctcg gaggagcgcg
     4081 cgtcgattct gccgaacgcc gatgcgctgt acaactcggc gacggtggcg cacgacgcgc
     4141 gctggggcct gccgctgccg tcgttcgcgg acaccgttgc ctaccgggac gaggtcctcg
     4201 aacgcgtggt gcgcggcctg gaggacatcg atccctattt cgtcgagctt gccctgcgcc
     4261 acgagctgat gcatgcggag gcgttccatt acacgcgcca gacgctgggt tatccgggac
     4321 cggaagcgga cgcaaggctg actgaaaata agggtgactt cgcgtgcccc ggcggcgtgt
     4381 tccggctcgg cgccaagccc ggcgagggct tcgccttcga caacgagaag tggagccacc
     4441 cagtcgtgct cgagccgttc cggatcgcgc ggcgccccgt gacttacagc gagtaccgga
     4501 agttcgcgca accgccctac tgcaaggacg gcatggtccg ccgtttcgat cgctggatcc
     4561 cgatcccgga ggacgagccg gtgcgccacg tgagctggca tcaggccaac gcgtactgcg
     4621 cgttcgccgg caggcgcctg ccgaccgagg cggaatggga gtgcgcggcg ctgtccgggc
     4681 tggagaacgt cggccacgtc tgggagtgga cttccagcac cttcctgccc tatcacggct
     4741 tcgtgcgcga cccgtacaag gagtattccg agccgtggtt cggcactcac aaggtcctgc
     4801 gcggcgcgag cttcgctacc cctgccggcg tatcgcacgc gcgcttccgg aatttctata
     4861 ccccggaacg ctccgacatg ttcgtcggct tccgcacctg cgcaccgtga gcgccctgac
     4921 ttcccgcgac aacccgaagg tcaagcattg ggcaatgctg gcgaaggacg cccgctaccg
     4981 gcgcacggaa aggcgcgcgc tgatcgaagg cccgcatctc ctcgccgccg cgctccagca
     5041 cggctacagg cccgttgcgg tgctggcgac ggagaaggcg gcggccggcg aggaaatcgg
     5101 cggactgatc gcccaatgcg gcgtgcgggc agtcatgctg gccgaaagcc tgttcggcgc
     5161 gatttcggac gtggagaccc cccagggaat agcagcggag atcgcgattc ccgacgaagc
     5221 cgaaggggag ggcctggata ccgtgttcct ggaaggcgtg caggatccca gcaacgtcgg
     5281 cgcgatcatt cgcagcgcgg cggggttcgg catcggaagg gtcctcctgg accgcgactg
     5341 cgccgacgcc tggtcaccca aggccttgcg cgccggcatg ggcggccact tcgccttgcg
     5401 ggtttacgaa accggccgcc tggccgcgga gatggacgcg ttcaagggaa cgctggcctg
     5461 cgccgtggcg cggggcggcg tgccgttgcg cgaggccgcg ctgagcggct ccatgggatg
     5521 gatcttcggc gcggagggaa ggggactaag cgaggaaacg ctccgcaagg taggcctgca
     5581 ggtgaccatt ccgatgaagc cgggcaccga gtcgctgaac gtcgccgccg ccgcggcgat
     5641 ctgcttctac gaggccttta gcaggtcaaa ttcggggacg gagccctaac tccacggctg
     5701 gcgcaacttt cggagttagg gctccgtccc catcggagtt agggctccgt ccccgaattt
     5761 cagtagatct ggcgcttgat ccgcagctcg cgcaggatcg tggtggcgat ttcctcgatc
     5821 gacttgctgg tggagttgat cgagcggatg ccctcccgcc gcatcaggtt ctcggcctcc
     5881 tcgacctcga agcggcagtt gtcgaggtcg gcgtagcggc tgccgggccg gcgctccttg
     5941 cggatctcgt gcagccggtc ggaggcgatg gtgaggccgt agagccggct tttctccccc
     6001 cgcagcgcct cggggagctt catgcggtgg aaatcctcgg ggatcagcgg gtagttcgca
     6061 gccttcacgc cgaactgcat ggcgaggtat aggctggtcg gcgtcttccc gctgcgcgag
     6121 acgccgacca ggatcacgtc cgccaggcta agctcgcggt gcgaggcgcc gtcgtcgtgc
     6181 gccatggcga aattgatcgc ctcgatgcgc tgctggtact ccttcttgtc catcgcgctg
     6241 tgagagcgcc cgatcgtatg gctcgacttg accccgaggc cggcttccag cggatcgatg
     6301 aagctttcga agaaatcgat aaacagcgca ttggcgcgcc gcacgaccgc gcgcacgtcg
     6361 ttgttcacga gggtggagaa cacgatcgcc tggccgttgc ccgcttcggc ggcgatgcgc
     6421 gcgatgcagt cctcggcctt ctcggtcgag tcgacgaacg gcagcgtgac ctggctgaac
     6481 tcgacgccct cgaactgcgt gagcaggctg tggccgagca tctgcgccgt gatgccggtg
     6541 ccgtcggaga cgaagaacac ggttcggcgc tgagccataa cgataaaata cccaacaatg
     6601 cgcgtagcgt ggcttaaaga tttacgcatg gccgacctcg agcaggtcgg cggaaagaac
     6661 gcttcgctcg gcgagatgat cggcggcctt tccgcagccg gaatccgcgt tccgggcggg
     6721 tttgccacca ctgcggatgc tttccgggaa ttcatggggg agctcgcgcc gcgcatcgac
     6781 gagcgcctga agaagctcga cccgaaggac gtcgacgcct tggcgaaatg cggcgccgaa
     6841 gtgcgctcct ggatcctggc gaggccgttt cccgccgagc ttgagcgaga gatacgttca
     6901 aaatatcaag atcttacatc tcagacttca cctgaaacct ctttcgccgt gcgctcctcc
     6961 gcaacggcgg aggatctgcc cgaagcctcg ttcgccggcc agcaggaaac ctttctcaac
     7021 gtccgcggcg ccgacaacgt cctcgacgcg atccgccatg tcttcgcttc gctctacaac
     7081 gaccgcgcga tctcctatcg cgcccaccac aacttcgacc acgctgccgt cgcgctgtcg
     7141 gcggcggtcc agcagatggt gcgcagcgac ctcggcgcga gcggcgtgct gttcacgctc
     7201 gataccgagt cgggcttccg ggacgtggtg ttcatcacct cgtcctatgg cctgggcgag
     7261 ctcctggtgc agggcgccat caaccccgac gagttctacg tccacaagcc gatgctggag
     7321 aagggccgcc cggccatcgt gcgccgcggg ctgggctcga agctgcagaa gatggtgttt
     7381 gcgaaatccg acaaggcggg caagtcgacc cgggtggtcg acgtcgcgga gaaggaacgc
     7441 cattcgtttt ccctgtccga caaggaaatc ctggagctgg cgcgctacgc cgtcgcgatc
     7501 gagcgccact acggccgccc gatggacatc gagtgggcca aggacggcac cgacgggcgc
     7561 ctctacgtgc tgcaggcgag gccagagacg gttaaatccc gcacgcgcga cgtcgaggag
     7621 cgctatgcgc tgaagggcag ctcgcgcgtg atggcgagcg ggcgcgccat cggccacaag
     7681 atcggcagcg gcgcggtgcg gatcgtcccc gacgcctcgc agatgtcgcg ggtgaagaag
     7741 ggcgacgtgc tggtcacgga catgaccgac ccgaactggg aaccggtcat gaagctcgcc
     7801 gcggcgatcg tcaccaaccg cggcggccgg acctgccacg ccgcgatcat cgcgcgcgag
     7861 ctcggcatcc ccgcggtcgt cggctgcggc gacgctaccc ggcgcctgaa ggacggccag
     7921 ccggtgaccg tgtcctgcgc cgaaggcgac accggcagcg tgtatgaggg cctgctgccg
     7981 ttcgaggtct cgacccgcga gcgcggcgag atgccgcgca tcccggtgaa gatcgcgatg
     8041 aacgtgggca atccgcagct tgcgttcgag ttcgcccaga tcccgaatgc gggcgtgggc
     8101 ctggcacggc tggaattcat catcaacaac gagatcggcg tgcacccgcg cgcgtgcctc
     8161 gactacgaag agctgcccaa gccgctgaag gccaagctgg acgaaaagac ccgcggctac
     8221 gccgatgcgc gcagcttcta ccgcgacaag ctggtcgagg gcgtggcgac catcgccgcc
     8281 gcgttctggc ccaagcccgt gatcgtgcgg ctttcggact tcaagtcgaa cgaatacaag
     8341 aagctcctcg gaggcgagcg ctacgagccc gatgaggaaa acccgatgct cggcctgcgc
     8401 ggcgcctcgc gctatctctc gcccgacttc cgcgcgtgct tcgagctcga gtgcgcggcg
     8461 atgcgcacgg tgcgcgacga gatcggcctg accaacgtag agctgatggt gcctttcgta
     8521 cgcacggtag gcgaagcccg cgaagtggtt tctttgcttg aaaaaaataa cttgcagcgg
     8581 ggcaagaacg gcttgcgcat cgtgatgatg tgcgagctgc cgtccaacgc catcctcgcc
     8641 gaggagttcc tcgagctgtt cgacggcttc tcgatcggct cgaacgacct gacccagctc
     8701 accctgggcc tggaccgcga ctcggggctg gtcgccggca gcttcgacga gcgcgacgcg
     8761 gcggtgaagg cgctgctggc gatggcgatc cgggcctgcc gcaagcatgg caagtacgtc
     8821 ggcatctgcg ggcaggggcc ctcggaccat cccgacctcg cgcgctggct gatggagcag
     8881 gggatcgaca gcctctcgct caacccggac acggtcgtct cgacctggat ggcgctcgcc
     8941 gaggctcccg cggcgaagaa gtcagcggct gcgtagcagc cgttgcttgt cgcggttcca
     9001 ttccttctcg cgctcggacg cgcgcttgtc gtgcttgagc ttgccgcggc cgagcccgac
     9061 ctcgagcttg atgcgcccct tgctgtagtg aaggttgagc ggcaccaggg tgtagccgcg
     9121 ctgctcgacc ttgccgatca ggcgccggat ctcctcggcg cgcaggagca gctttcgggt
     9181 gcgcgtgggg tctggcgtga aatgcgtcga gaccgtgggc agcgggctga tattcacgcc
     9241 gatgagatag agctccgcgc cgcgcaccat cacgtagccc tcggcgatgg tcgcgcggcc
     9301 cccgcggatc gccttaactt cccagccctc gagcgcgagc cccgcctcgt agcgctcctc
     9361 gatgaagtag ttgtgaaagg ctttgcggtt ctcgacgatg gacatgaggg caacaaccag
     9421 ggcaacaacc agggacagac cacgtttttc gtgagcgccc cgtccgctgc tgcggtaaaa
     9481 cgtggtctgt ccctgatttt cgcatgaagc agattgcgcg ctcggcgatt gtcgagcatt
     9541 ccgccgcaga aatgtacgcg ctggtggagg acatcgaggc ctatccgggc ttcctgccct
     9601 ggtgctcggc cgcggtggtg cacgagcgca gcgcgggcac gaccaaggcc acgctgaccg
     9661 tcggcctgcc ggccttgcag caatccttca ccacgctgaa cgagaaccgg ccgggcgagg
     9721 cgatcgacat gcggctggtg gaagggccat ttcgccgctt cggcgcgcag tggcgcttcg
     9781 tgcccttgtc gccgcaggcc tgccgcatcg agttctcgct gcagtacgaa ttctcccgcc
     9841 gcacgctcgg gcggctgctc gcgccgcttt tcgacggcat cgccgactcg atggtcgacg
     9901 cgttcgtgcg ccgggcggcg caggtctatg aaggttgagg tggtctatgc gcgccccgag
     9961 ggggcgcagg tggtgcgcct cgagctacca gccggcgctc ggttgcgtga tgccgtctcg
    10021 gcgtgcggtt tcaaaattga agaccacgca gtaggaatct tcggcaagcg cgtggcgctc
    10081 gatcatccgc tcgccgacgg cgaccgggtg gagatctacc gcccgctcgc gatggatccc
    10141 aaggaagcgc gccggcggcg cgccagcaag acaaaacgct agcggcttac ttgcaccact
    10201 cctggacact ctggcgcgcc ctggcggtct cctgcgcggt ctgcgcgtcg tcgaggaaat
    10261 agcggtcgcc cttcgaatcg gtgcgggcga tgcgctggcc gctttcgagg gagcgcagcg
    10321 cgtcctgggc gcgcgcgcaa ttcgcgcgtt tctcctgcgc gtcctgatcg gccttggcct
    10381 gcttctcgcg atccttctcg gcctcttcct ggcgcttgcg gaacgcggcc tcggggctga
    10441 gcggcttgtc gccgtccttc ttcgcggctg cggcgggtgc ggcgggagac gccgatcccg
    10501 gcggcggcgc cctcatcggc gtggcggtca cgccgggcgg cggcacgtcg ccgtagcgca
    10561 ccttgccgtc cttgtcgacc cacttgtatt gctgcgccga gactgcggcg gcgaagaaga
    10621 agctcaagac aagaagtttt ttcatgagcg agaaacgcgc gtcgattcag atggatgaca
    10681 gtatcgcgta taatgccgct ttgcgagcat gaaaaaatgc gcgtgattca gaaggcgctg
    10741 accttcgacg acgtccttct cgttcccgcc cactcgcgcg tcctgccgcg cgaagcctcc
    10801 ctcaagaccc gactcacccg caagctccag ctcaacctgc cgctcgtctc cgcggcgatg
    10861 gacaccgtga ccgaggcgcg cctcgcaatc gccctcgcgc aggaaggcgg catcggcatc
    10921 gtccacaaga atctcccgcc caaggcgcag gccgccgaag tggcgaaggt gaagcgcttc
    10981 gagtcggggg tgctgcgcga cccgctgacg gttccgcccg agatgccggt gcgcgacgtg
    11041 atcgcgctgc agcgcgagcg caagatctcg ggcttcccgg tggtgaagaa cggaagagtg
    11101 gtcggcatcg tcaccaaccg cgacctgcgc ttcgagaccc agctcgacca gccggtgaag
    11161 gcgatcatga cgccgcagaa gaagctggtt accgtgcgcg aaggcgcgag ccggcaggaa
    11221 gcgaaggagc tcatgcatcg ccaccgcctc gagcgcgtgc tggtggtcgg cgaggacttc
    11281 gagctgaagg gcctggtcac ggtaaaggac atcaccaagg aaagcgagca cccgaacgcc
    11341 tgcaaggatg cgcaaggaaa gctgcgcgtc ggcgcggcgg tcggcgtggg cgagggcacc
    11401 gaggagcgcg tcgcggcgct ggtggaagcg ggcgccgacg tgctggttgt cgacaccgcg
    11461 cacggccatg cgcagggcgt cctcgaccgc gtcgcctggg tcaagaagaa cttcaagatc
    11521 gaagtgatcg ggggcaacgt cggcacgggc gacggcgcgc gcgcgctcgt cgaccacggc
    11581 gccgacggcg tcaaggtcgg catcggcccg ggatcgatct gcaccacgcg catcgtcgcc
    11641 ggcgtcggcg taccgcagat caccgcgatc caggacgtgg ccgaagcgct caaggatgtg
    11701 ccgctgatcg cggacggcgg cgtgcgctac tccggcgaca tcgccaaagc cctcgccgcc
    11761 ggcgcgcacg cggtcatgct cggctcgctc ttcgccggca ccgatgaatc gcccggcgag
    11821 atcgagctct accagggccg ctcgtacaag tcctaccgcg gcatgggatc gctcggcgcg
    11881 atgcagaagg gtgcggccga ccgctatttc caggactcgg agatgaacgt cgacaagctg
    11941 gtgcccgaag gcgtcgaggg ccgcgtgccg tacaaaggca gcgcgatctg ggtgatcgag
    12001 cagctcatgg gcggcgtgcg cgcgagcatg ggttacacag gctgcagcac catcgaagag
    12061 ctgcgcaccc gcacgcggtt cgtcgagatc accgctgcgg gaatccgcga atcgcacgtg
    12121 cacgacgtgc aaatcaccaa ggaggctccg aattaccggg cggaataagt agtgcaccag
    12181 aaaatcctca tcctggactt cggagcgcaa tacacgcagc tgatcgcccg ccgggtacgg
    12241 gaagcgaagg tctactgcga gatccatccc tgggacgtcg acgacgcctt cgtgcggcgc
    12301 ttcggcgcgc aaggcatcat cctctcggga agccacctct cggcctacga cgatacccgg
    12361 gcgcacgcgc ccaaggcggt gttcgaagcc ggcgtaccgg tgctgggcat ctgctacggc
    12421 atgcagagca tggcgcacca gctcggcggc agggtcgagg cgggcacggt gcgcgagttc
    12481 ggatacgccg aagtgcgcgc gcgtgggcac accaagctcc tggacggcat cgaggactac
    12541 cgcacccctg aaggccacgg catgctcaag gtgtggatga gccacggcga caaggtcgcc
    12601 gagctgccgc cgggcttccg gctgatggcc tccaccgaag cctgcccgat cgccggcatg
    12661 gcggacgaag cgcgccgctt ctacggtgtg cagttccacc ccgaggtcac ccatacccgg
    12721 cagggaacga agctgctgca ccggttcgtg cgcgatatct gcggctgccg gggcgattgg
    12781 caaatgccgg aatacgtttc agaggccgtt gcaaaaataa gatctcttgt cggaaaggac
    12841 gaagtcatcc tcggcttgtc cgggggcgtc gattcctcgg tcgccgcggc gctgatccat
    12901 cgcgccatcg gccctcagct cacctgcgtg ttcgtcgacc acgggctgct gcgcctggac
    12961 gaggcaagac aggtcatgga cacgtttgcg aagcacatgc acgtgaaggt cgtgcatgtc
    13021 gacgcatcgg cccagttcct gtccgcgttg aaaggcgtcg ccgaccccga ggagaagcgc
    13081 aagatcatcg ggcgcctgtt cgtcgaggtg ttccaggcgg aagcgaagaa gatccgcaac
    13141 gccaagtggc tcgcgcaggg aaccatctat cccgacgtca tcgagtccgc cggcgcaagc
    13201 accaagaagg cggccaccat caagtcgcac cacaacgtcg gcggcctgcc cgagacgctt
    13261 cacctgaaac ttctcgagcc gctgcgcgag ctcttcaagg acgaggtgcg cgagctcggc
    13321 ctggcgctcg gcctgccgcg cgagatggtg ttccgccatc cgttcccggg accgggcctg
    13381 ggggtccgca tcctcggtga agtcgagaag gagcacgtgc ggctgctgca gcgcgccgac
    13441 gacatcttca tccaggagct gcggagctcc gggtggtacg acaaaaccgc gcaagccttc
    13501 gccgtgttcc tgccggtacg ctcggtgggc gtgatgggcg acgggcgcac ctacgagaac
    13561 gtggtggcgc tgcgcgcggt gcagaccagc gacttcatga ccgcggactg ggcgccgctg
    13621 ccgcacgagc tcctcgcgcg cgtctcgaac cgcatcatca acgaagtgcg cggcatcaac
    13681 cgcgtgacct acgacatctc ctccaagccc cccgccacca tcgagtggga gtaggcgcag
    13741 aggcaggccg cgctcgcggc ccgccatccg ggtaagactt acttggtcgt cgagctgccg
    13801 gagctgctgc cgctcgagct acccatgccg ctgctcgagc ccgtgctggg caactgggtc
    13861 gtgctcggcg aggaggtcga gctgccgctg ctgctgccgc cgctcgctgc gccggcactg
    13921 gagcccgatt tgtcgaagtg agcgtcccat tcggcacgcg acacttgccc gtcgttgttc
    13981 gcatccaccg ccgagaaagt cggagcggag gtgctgccgc tcgacgacgt gctggatccg
    14041 cccgacatgc cggacgagcc actgcccgac gccgcagggg aagacgagga gctatcgccg
    14101 ctcgtgctgc tggatgaaga gctttgcgcc aaagcagcgc cggacaaggc gaacagcgct
    14161 gcggctgtgg ccgcgctcag gagtttcagg ttcaacattg gtatatctcc ttcggatgaa
    14221 atgggaagag gtgcgccttg ctcgatgcaa ggctcgttcc ctttcatccg gcgtcagcat
    14281 tggctgcggc cgcgcgatgc cacagcgaat ggcgccagcc gtagccgatg gcgaaggtcg
    14341 ccgccagccc gatcgcggaa tagaggagcg tggtcgccgc gaagccgatc agcgcgatga
    14401 tcggcccggc cgcgag
//
LOCUS       DATBSZ010000205         3744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1605966, whole genome shotgun sequence.
ACCESSION   DATBSZ010000205 DATBSZ010000000
VERSION     DATBSZ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605966"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VIG70_13175"
     CDS             complement(<1..250)
                     /locus_tag="VIG70_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251551.1"
                     /translation="MSRLLVALAFVSMSAAAQPQLKPLSVIVFPGGFNLPVWAAERQG
                     FFEANGVRVALTPTPSSTFQMQGLAEGRFDIAMTAFDNV"
     gene            complement(247..789)
                     /locus_tag="VIG70_13180"
     CDS             complement(247..789)
                     /locus_tag="VIG70_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8251552.1"
                     /translation="MAALTREKIQDGIADTGNEKSVVRPYVMSHGTMECYSLKESRKF
                     YEEFLGLECVRHAKPAMVVRCGLKFHIVAVEVGAAVHPQNVLNHWGLDVGSKEEVDRV
                     HREATKQKDKYKIRQVLPVVDQHGVYSFYMEDLDHNWWEVQYYPGFQNEDLFDFGDRF
                     SMDDGAEVGELKELNIKTTA"
     gene            complement(798..1802)
                     /locus_tag="VIG70_13185"
     CDS             complement(798..1802)
                     /locus_tag="VIG70_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004541759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEY8251553.1"
                     /translation="MAHRITLKPSGHSYEAEEGKSVLQAGLDAGHMLPYSCRAGVCRT
                     CRGTIVEGKVDYGLVHETYLPESDKAKGYALLCQAKALSDLVVEVREVQGVRPRVIPC
                     RVERLEKPAPDVAVIGLRLPMNENFRFLAGQYIDFLLKEGKRRSYSLAVRPDPGGVTA
                     LEIHVRHTPGGVFTDHVFASLKVRDLLRFEGPLGSFYLREDSAKPIVMVASGTGFAPI
                     KALCEHALEKKISRPITLYWGVRLKRDLYMLDLPRSWPHVKFIPVLSDETWDGRMGFV
                     HRAVMEDFPDLSGHQVYACGAPIVVESARRDFSAQCKLPPDEFFADSFLTEADKAVTA
                     "
     gene            complement(1802..2506)
                     /locus_tag="VIG70_13190"
     CDS             complement(1802..2506)
                     /locus_tag="VIG70_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251554.1"
                     /translation="MYEVSDVTVNYGAAAAVSNVRLRIEAGELVVVVGPNGAGKSTLI
                     NAMAGLQAVASGRLLVEGNDITREAPHRFCERGIAIVPEGRRLFVNLSVRENLELGSY
                     RAGARSARAVSLARVCALFPVLAERMDAPAGALSGGQQQMVAIARALMARPKFLLLDE
                     PSLGLSPMITSDMFRAVREINAEGVAVLLVEQNVRAALEIARRAYVLDEGRIAAEGEP
                     RQLMSQPQIQRAYLGT"
     gene            complement(2499..>3744)
                     /locus_tag="VIG70_13195"
     CDS             complement(2499..>3744)
                     /locus_tag="VIG70_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEY8251555.1"
                     /translation="MLAAAALTLWTAYAIYHSRFGTGLFAIHDDEDVAEVMGVPTYRF
                     KLAAFAISCALAGLAGGVHAVFVSYVTVAETFSIALAVNVVLMAALGGTRHWLGPAVG
                     AVAITVLLYAFTGGDAAVLGRALTGLILIVVIVAMPHGILGQILKRFRIKTPARAVVR
                     ADDAPRQAVTATTEPLLVARGVTKAFKGVQALAGANLEVRRGEILGLVGPNGSGKSTL
                     INVVSGHYRADAGSIRFEGDELVGTPAHRIARAGIARTYQIPRPFPQLTVLQNVALPP
                     MFGQVVMDRVRAEEEAWRWLAFTGLEAKAHRLPSDLNLHERKFLELARALASRPKLLF
                     LDEVLSGLTPSEMDGALRLIRAIRDQGATIVFVEHVMRAVMELTDRVAVLTYGRAIAE
                     GAPAEVMRHPDVVSAYLGTAHV"
BASE COUNT          634 a         1272 c         1225 g          613 t
ORIGIN      
        1 cgacgttgtc gaacgccgtc atcgcgatgt cgaagcggcc ctcggccagg ccctgcatct
       61 ggaaggtcga gctcggcgtg ggggtgagcg ccacccgcac gccgttcgct tcgaagaagc
      121 cctgccgctc ggcggcccag accggcaggt tgaagccgcc cggaaagacg atgaccgaga
      181 gcggcttcaa ctgcggctgg gctgcggcac tcatggaaac aaacgcaagc gcgaccagca
      241 ggcggctcat gccgtggtct tgatgttgag ctccttcagc tcgccgacct cggcgccgtc
      301 gtccatcgag aagcggtcgc cgaagtcgaa caggtcctcg ttctggaagc cggggtagta
      361 ctggacttcc caccagttgt ggtcgaggtc ttccatgtag aacgagtaga cgccgtgctg
      421 gtccacgacc ggcagcacct ggcggatctt gtacttgtct ttttgcttgg tcgcctcccg
      481 gtgcacgcga tcgacctctt ccttggagcc gacgtcgaga ccccagtggt tgagcacgtt
      541 ctgcggatgc acggcggcgc cgacttcgac ggcgacgatg tggaacttga ggccgcagcg
      601 caccaccatc gccggcttgg cgtggcgtac gcactcgaga ccgaggaact cctcgtagaa
      661 cttgcgcgac tccttcaggc tgtagcactc catcgtgccg tgcgacatca cgtacggcct
      721 gacgacggac ttctcgttgc ccgtgtcggc gatgccgtcc tggatcttct ctctcgtcag
      781 tgcagccatg tctcctccta tgcggttacg gccttgtcgg cctcggtcag gaatgaatcg
      841 gcaaaaaact cgtcgggcgg cagcttgcac tgcgcgctga agtcgcgccg cgccgactcc
      901 accacgatcg gcgcgccgca ggcatagacc tgatgccccg acaggtcggg gaagtcctcc
      961 atcacggcgc ggtgtacgaa gcccatgcgt ccatcccacg tctcgtcgga aagcacggga
     1021 atgaacttca cgtgcggcca ggagcggggg agatccagca tatacaggtc gcgcttcaga
     1081 cgcacccccc agtagagcgt gatcggccgc gaaatcttct tctcgagggc gtgctcgcag
     1141 agcgccttga tcggcgcgaa gccggtgccg ctcgccacca tcacgatggg cttggccgag
     1201 tcctcacgca ggtaaaagct gccgagcggc ccctcgaagc gcaggagatc cctcaccttc
     1261 aggctggcga acacgtggtc ggtgaacacg ccgccggggg tatggcgcac gtggatctcg
     1321 agcgcggtga ccccgcccgg gtcgggacgc acggcgagcg aatagcttct tcgttttccc
     1381 tctttcagga ggaagtcgat gtattgcccg gccaggaacc ggaagttctc gttcatcggc
     1441 aggcgcaggc cgatcaccgc gacgtccggc gccggcttct ccaaccgctc cacccggcac
     1501 ggaattacgc gcgggcgcac gccctggact tcgcgcactt cgaccaccag gtcggagagc
     1561 gctttcgcct ggcagagcag cgcatagccc ttcgccttgt cgctctccgg gagataggtc
     1621 tcgtgcacca ggccgtagtc gaccttcccc tcgacgattg tgccgcggca ggtgcggcac
     1681 acgccggcgc ggcagctgta gggcagcatg tggccagcgt ccagcccggc ctgcaggacg
     1741 ctcttgcctt cctcggcttc gtagctgtgc ccgctgggct tgagagtgat tcgatgagcc
     1801 atcaggttcc taagtatgcc ctctggattt ggggttgtga catcagttgc cgcggctcac
     1861 cctcggcggc gatgcggcct tcatccagga cgtaggcgcg gcgcgcaatc tcgagagccg
     1921 cgcggacgtt ctgctccacc agcagcaccg cgacgccttc tgcattgatc tcgcggaccg
     1981 cacggaacat gtccgaggtg atcatcgggg aaagtccgag cgaaggctcg tccagcaaga
     2041 ggaacttcgg gcgcgccatc agcgcccggg cgatcgcgac catctgctgc tggccccccg
     2101 agagcgctcc ggcgggggca tccatgcgct ccgcgagcac cgggaagagc gcgcagacgc
     2161 gcgcgagtga aacggcgcgc gcactgcggg ccccggcgcg gtagctgccg agctccaggt
     2221 tctcgcgcac gctgaggttc acgaacaggc gccggccttc gggcacgatc gcgatgcccc
     2281 gctcgcagaa gcgatgcggc gcctcgcgcg tgatgtcgtt cccctcgacc agcaggcgcc
     2341 ccgaggccac cgcttgcagg cccgccattg cgttgatcag cgtcgatttt ccggcgccgt
     2401 tcgggccgac caccactacc agctcgccgg cctcgatccg caggcgcacg ttgctgaccg
     2461 ccgctgcggc accgtaattg accgtgacgt cgctcacctc atacatgcgc ggtgcccagg
     2521 taggcggaga cgacgtcggg gtggcgcatc acctcggccg gcgcgccctc ggcgatcgct
     2581 cttccgtagg tgagcaccgc gacgcgatcg gtcagctcca tgaccgcgcg catcacgtgc
     2641 tcgacaaaca cgatggtcgc gccctggtcg cggatcgcgc ggatgaggcg cagcgcccca
     2701 tccatctccg atggcgtcag gcccgaaagc acctcgtcga ggaacagcag cttcggccgc
     2761 gacgccagcg cgcgcgccag ctcgaggaat ttcctctcgt gcaaattcag atccgaagga
     2821 agacgatgcg cctttgcctc cagtccagtg aaggcaagcc agcgccacgc ttcctcctcg
     2881 gcgcggacgc gatccatgac cacctgtccg aacatcggtg gcagcgcgac gttctgcagc
     2941 accgtcagct gcgggaacgg gcgcgggatc tggtaggtcc gtgcgatgcc cgcgcgcgcg
     3001 atgcgatgcg ccggtgttcc gacgagctca tcgccctcga agcggatcga gccggcatcc
     3061 gcccggtaat ggccgctcac cacgttgatc agcgtggact tgccggagcc gttgggaccg
     3121 accaggccga ggatctctcc acggcgaacc tcgagattcg ccccggcgag cgcttgcaca
     3181 cccttgaacg ccttggtgac gcctcgagcc acgagcagcg gttccgtcgt ggccgtcacc
     3241 gcctgccgcg gcgcgtcgtc ggcacggaca acagcgcggg cgggtgtctt tatcctgaag
     3301 cgcttgagga tctgcccgag gatgccgtgc ggcatcgcga cgatgacgac gatgaggatc
     3361 aggccggtca gcgcgcggcc gagcaccgcg gcatcgccgc cggtgaaggc gtacaagagc
     3421 acggtgatgg ccaccgcgcc gaccgccggc ccgagccagt gccgggttcc gccgagcgcg
     3481 gccatcagca ccacgttgac cgccagggcg atcgaaaaag tttccgccac ggtcacatag
     3541 gaaacgaaca cggcgtgaac gccgccggcg aggccggcaa gcgcgcacga gatggcgaag
     3601 gcggccagct tgaagcggta ggtcggcacg cccatcacct cggcgacgtc ctcgtcgtcg
     3661 tggatggcga agagacccgt gccgaagcgc gagtgataga tcgcgtaggc ggtccacagg
     3721 gtcagcgccg cggcggcgag catc
//
LOCUS       DATBSZ010000206        28492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1127993, whole genome shotgun sequence.
ACCESSION   DATBSZ010000206 DATBSZ010000000
VERSION     DATBSZ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 28492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28492
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127993"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2575)
                     /locus_tag="VIG70_13200"
     CDS             complement(<1..2575)
                     /locus_tag="VIG70_13200"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HEY8251556.1"
                     /translation="MAATKRIKAETQAPLNLRPRATALAVSAALLPWGSAHALPGDPL
                     TTVGTVVHSQPTSHTMRIDVTTQHAATSYRGGFSIAAPETVNIHQLGRNSVFLVRDIS
                     GNPTNIFGTLTANGQVFLSNSNGVLFGRTASVEVGALFATTLSITDENFKNGRYQWSR
                     EGTAGSVVNEGRIVTTNGYTALVGPQVRNDGIIVARAGTVALAAADRVSLDLVGDGLI
                     SINVEQAVVNASVVNTGSIEADGGRVFLSARSANALLDTVINNSGIIRANSLVERNGE
                     IVLDGGSAGVVAVSGNGRLEAAGADAGTTGGTVKVLGKYVGLFDKARIDASGDAGGGT
                     VLIGGNYQGAGPEANASMTYVGTKARIMADALRSGNGGTIIVWADGTTRFYGTGSVRG
                     GAASGNGGFAEVSGNGHLDFQGSVDRRAPNGKAGMLLLDPTDLTIVAGTASTNVTTVT
                     PFEPATASGSTIGWDEIDAALGGGDVSVRTTTGFDAGEAGNITILGAGTLNNGNNLSI
                     IAQGDLVQSTGATITRTVAGGLNLTAATGNVALNDDITLTSSALTVASSTFSQAAGTI
                     DAGGLLTINVSGASSQAAGAVITGAGGLTKAGAGTLTLSGTNTYTGATTVSAGNLTLQ
                     GGAAIADAGVVALNGGTLEIDAAETIGRLSGAGGTSVVLDATLTFGDATDTTIAATIS
                     GSAGLTKQGASTVTLTGNNTYAGTTAIDVGVLNIQHANALGATAGGTTVASGAALELQ
                     GSIAVGAEALTITGAGPAGGALRNVSGNNSFGGVITLAGTARINSDANLLTLTGGISG
                     AQNLTVGGAGDTTQSGAIATTTGTLTKDGAGTLTLSGTNTYTGTTTISAGNLTLQGGA
                     AI"
     gene            complement(2684..4435)
                     /locus_tag="VIG70_13205"
     CDS             complement(2684..4435)
                     /locus_tag="VIG70_13205"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEY8251557.1"
                     /translation="MRVRLSRESDARLRNAGLIARPIVVAVLSLASAVAIAQQRPDAG
                     QILEQQRQPLRLPPPPDDDIRPRPPEPRPALPVSPTLKVRISKFTFSGNTLYEDATLA
                     QVVEEFVGKELDFEGLTDAATKVRAYHRERGYFLAQAYLPQQAIRDGVVEIAVIEGRV
                     GIVELQRRPATRLAEWLLAGILNAHLKAGDIITETGLEKPLLLINDLPTAAVTSEIRP
                     SQTVGAADLRVNVDQGVGLLNGYVDFDNQGSRFTGAYRLGGSLNVNNPLTIGDQLSLR
                     AFATDEEMYFGRIAYIVPAGFWGTRIGASVTKFSYKLGDEFASLEANGDGLVKTVFAF
                     HPLYRTRNTNLIGQFAYEDKRLFDRIDSTATVEDRFIDSIKAGVVGDFRDRFLSGGLN
                     AFSVTYTQGDLKLAPTALAQADAAVTGLNTVGTFRKWNYDFKRLNRVTDNLSVLLALT
                     GQQASKNLASAEKMSLGGANGVRAYPVGEANGDSGNLVQSEVRYIWPGVKVFEGDVVV
                     MALYDWGQVDVNEKPLPTETKNKRSISGYGVGASLGREGEFIVRASASWRVGDEVPTA
                     DPESRNPRVWIQAVKWF"
     gene            4962..6014
                     /locus_tag="VIG70_13210"
     CDS             4962..6014
                     /locus_tag="VIG70_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251558.1"
                     /translation="MVGAVASSGVGAGLSVLVAQTRLRERQQLFLRRALYLGLAISAP
                     VGVATAIVGWNLSERLGGAALAPQSVVLAALCGWIAVIHGLVNSFWLGQQRRDLMLAL
                     ALLAAALALAAAALAPPAFVLELIIVAQAAPAAVLLFVPHRAAAPTRVEDHALERYVL
                     PGVVIGILSPASMIVARSLVADALSWHESGVLQALWRVSDWICGFASGVLSVLYLPRF
                     AAAYPQARLASVLRESARTVLLPSAVLFLLLFAFYSPVLEALYDPSFRAAPLAVGLLF
                     AGSLARIAAWIPLFGLYAATRTRAIAVGELLSLPLFAALTYAAGDGLTLEIVGALWLA
                     SYLAYGAFNFWALRKA"
     gene            complement(5986..7206)
                     /locus_tag="VIG70_13215"
     CDS             complement(5986..7206)
                     /locus_tag="VIG70_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8251559.1"
                     /translation="MSAAVLPPHPVSRNAWRELWLITIGHGLTHWYPATFYLLLPLIG
                     SELGLSYSQIGLIMTCQYIASAIANVPGGVLVDTVGRKGLLMAVSLFWIGFPYLLIGF
                     THSYAMLLACVALVGFGNSLWHPTAIPTLGRRYPERKGLVLSVHGMGGNVGDAVAPVI
                     IGAALTYFSWREAVVLNVVPGLVVAALLFVFLGSMRLGAKKSEHEAQSFTHYLAGLKD
                     LLASRALVLLSTGSAFRTMTQTTLLTFLPVYLAHDMGYSPFWVGACLFALQAAGFAAS
                     PIAGHLSDRMGRKQILVSSMAATAVVLAAMAFSGGSPLFIALVAVLGFFLYATRPVIQ
                     AWMLESTPKNMGGSSIGVLFGAQALGGAIGPLLGGIVADRFGLLATFYFLAATIVVAN
                     LFVFGVTPSAAPRS"
     gene            7235..8401
                     /locus_tag="VIG70_13220"
     CDS             7235..8401
                     /locus_tag="VIG70_13220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8251560.1"
                     /translation="MAVYPVVLLSVLTSIGLRGSKVAVSLYALELGAGAAAVGALAAL
                     FSVLPLLLAVPAGRIADRFGMRMPIVLGTAIMAGGLALPLAAPGLGWLFLCPTLIGLG
                     HVFFHVSVHNLVGSLGDGAARARNFATFALGISIAAFIGPSAAGFAIELAGFQAAFAL
                     LACIAAVPAFYALFHRSLFPPRQRLEGEHAAGSAFELLSIPALRRTLILSGVALTGIE
                     LFSFYLPIYGRAIGLAPSTIGMILSSYALAGFVVRIVMHRLTQRYGELGVLSGSLFAA
                     AGAYLAVPALSDAWLLAGAAFALGLALGSAQPLTIALTYGYAPPGRSGEALGMRIMAN
                     KITQIAVPLAFGGFGAVLGAAPVFLATGAFLLAAGCLSVRGAGSRLARIQETRS"
     gene            8398..9678
                     /locus_tag="VIG70_13225"
     CDS             8398..9678
                     /locus_tag="VIG70_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8251561.1"
                     /translation="MRADQNELLTRTAAGTPMGELFRRYWLPALLASELPTPAGPPVR
                     VTLLSEPLVAFRDSANRLGLIDEFCAHRGASLWFGRNEEGGLRCAYHGWKYDAGGQCV
                     EIPSEPDNPKLCRRMKLRSYPLIERGGVLWTYMGPRERQPPPPEHEWAMVPDAHRFVS
                     KRLQECNYLQAMEGGIDSSHVSFLHRHTITTDPLFRGSKGNEYNLQDLRPHFEVVESP
                     GGLYIGARRGTGDGQWYWRITQWVMPSFTLIPPRGDHPIGGHCWVPMDDVHCWVWSSN
                     HHATRALTPEERAALEAGKGIHAPLIPGTFRPVANKDNDYLIDRRAQEEGRSFSGVEG
                     FAMQDASVQESMGRVQDRTRENLVPTDLGIVMARRRLIAASKLVQKGKSPPGADPRHQ
                     RVRSVAIVLPQGVAFSEAAGGALVPTPGSAHTTV"
     gene            complement(9663..11168)
                     /locus_tag="VIG70_13230"
     CDS             complement(9663..11168)
                     /locus_tag="VIG70_13230"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY8251562.1"
                     /translation="MSWEFIAGQFAAQFALWWIIWLGILLGIVVGILPGFSPQNTLIM
                     LLPLTLGIPVEQAFAFMVSLYCANHLAGGVPAILIKIPGSGGAAATTLDGYPMTQKGQ
                     AQQAIVLSFTASVFGGLITTLATLALLPWLSQIGLYLRSVEMVVVMLFGITLIAVIAA
                     KDTLKGLIAGLFGLLIGVIGTDHIYNAPRATFGFLELYDGVPLVAALIGLFAVSEALV
                     MLEEKLIVSSEGRERAMRASWADTLDGLRLTVVHWWHIVWTSFVGLFIGIVPGAGASI
                     AAFVAYQQSRMWSKHPELYGTGVPEGVIAPEAANNGVTSGTLVPMMALGVPGGTTSAV
                     MMIVLQYHGMVMGPRLFSTNPELAYGVFVAMAVAYIFMIFTILPLARYMARIVLIETT
                     YLAPIIILVTVVAAFVERQYLFDMGLALVFGVIGYIAHKTNYHVTAMLIGIILGPLFE
                     QYLARALRISQGDLTILFSSTLGNVLWGFLVLSLVLPLWRSYRRAARQTVV"
     gene            complement(11165..11641)
                     /locus_tag="VIG70_13235"
     CDS             complement(11165..11641)
                     /locus_tag="VIG70_13235"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY8251563.1"
                     /translation="MSRKAPLGADLVIPALALGFAGYFFWSITDLAWEAKANGVVIGT
                     ALIALVVVQVARIALEVAQGRGDLRTDPLWQPRDVLLKRIGMVAVTVAFILLLQVLGL
                     TLSLFASMAVALWLMDVRRPSLIFGISFGVAAAAYLLFIAVLDAGFPHGFIEKIFS"
     gene            complement(11644..12648)
                     /locus_tag="VIG70_13240"
     CDS             complement(11644..12648)
                     /locus_tag="VIG70_13240"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY8251564.1"
                     /translation="MKQANQSRRRLLAGASAALALGPLGARAQGFPSKNIRVVIPTGQ
                     GGGAERLARSFDEVWSKLLKTNFEYSFYAGAAGQVGYETFVHKREKDGYNLLFGNMGP
                     EMIMYALQKPNYRFPEDFQYFCRTDIDDSGVFSLRKGKFQRVEDLVAEAKKRPVNVAV
                     SRLPHPASIGMLALGAALGARFNLIPYGGGNPTTVAVLNGEADCGALPIAGIIAQREQ
                     LKVLAVFNDENPLAAQSDNAPPINKVFGTKLPELSSSRSWAIHTEAIEKYPERFAQLE
                     KTSKQVVNDPAFKEAFAKTGAPVEAIQYGDRTVCTRYAKGMVELANEYRSLLTAKKKG
                     "
     gene            12710..15439
                     /locus_tag="VIG70_13245"
     CDS             12710..15439
                     /locus_tag="VIG70_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251565.1"
                     /translation="MKRWLAVALLAWGPAALAQWNELSFVDPSLRWRTLETANFAVHF
                     AEQHRAQARTVAGTAERILPGMTALLRWRPESRIHVVVLDSADFANGLASPLPFNYTM
                     VFLSPPDEGELLQNREWLELVLTHELFHLVHLDMARAAPLGLRRVFGRVPFLFPNLLQ
                     PRWVVEGMAVHAESDPARGYGRLGQAHFEGMMRAEAARGLRSLEEINAGGRGFPLNRD
                     YLYGGYFFAFLQERYGREGLTAFIENYSDNIIPFRVHSNPVAATGKNMDQLWAEYHNW
                     LTARFSPKVASARQGEVIAQGWSITSPALTPDGTRWYISGSGYTLPRLVRQAPGGKPQ
                     ALRDVELDARLSASPGGGLLLAQPEICGNYNYYYDFNRVGPDGSLKRLTECGRYRIGA
                     PLDDGRTVGVRTENGLAQVVTLEGEVLYRAAAGESITGVAASGASVFVTRLRDGTWSL
                     IRIAGQNTEVLLSDSAIKHSPRLDAAGEVFFIADYGKVFNVWSLRGGRLARWTEAAHG
                     VREMSAPHGGELLLTTIEAEGDTLRTYRLPDSPLQDMSIPASEPSPSSTMPSTEAPDR
                     AYSPWSSVLPRSWLPLIEIADGAVKLGVVTFGQDALGLHQYAVTPVIELTQQELLGNA
                     LYVYDGRHQLLLDRTMRVRETVDDEVEAYTIEEGAQWVSTWRHLSLNRRFYWGLGGAL
                     ERERLHRAGGPTLSEQDERVLGLVAGLDTRRAQWLSEGPSQGMQVRLFAETSHGLHAA
                     YSGDVYRVDWRLHFPLGRTVVSLRWNEAWGEPDAEPFQLGGSDSDPPTLLPILNQRDF
                     ALRGYGSGEPSLLGHRARLGTLEWRVPLKDLDRHGMVPPVGFNRFAMNLFFDVGDAWR
                     RGAEPDYHRGFGIELMSEIRIGYVFGYDFRLGVAKGRDQGGETTAYLRLGRSF"
     gene            complement(15436..16098)
                     /locus_tag="VIG70_13250"
     CDS             complement(15436..16098)
                     /locus_tag="VIG70_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8251566.1"
                     /translation="MFKRLLLLSLALLAGSFSAGCGDSRSHAQAVYVLIDTSGTYSQE
                     AGRAQVIVNYLLGTLQPGDSLAVARVESRSFTEKDIIAKATFDARPSQANAQKRAFRE
                     KTDGFLKNVKGSRYTDITGGLLQGAEFLNETGAGTKTIVVFSDMQEEIDKITVRDIPM
                     NLKRIRVVAVNVVKLKTDNIDPRRYFGRLEAWQKRVVAAGASEWRVINDLERLDAIFS
                     RS"
     gene            complement(16101..17552)
                     /locus_tag="VIG70_13255"
     CDS             complement(16101..17552)
                     /locus_tag="VIG70_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251567.1"
                     /translation="MTETSFWMLWPDSPALSIFVVAVVALVFLYAARKPVHELLRAVG
                     HLIGGPLRLGARWLIATAEQMKERNRAVLLAHGRLEVGQRIEREFERLGAVVRRDLQG
                     YPTLQRKLLDEITRIEEDYKKCGEVPPPPPDWVEAVTAMANVKTGGSEMVQRILEEIK
                     ASIAKIHDKAIGEYRRSYETRHKILESFMPFWRSVDRNMSEVNKKLGDLQESAKGVDG
                     LMAKYEAINAKTDKAEHVLTISAFTQFAIALLVMAVAAGGAFINFKLIALPMSEMVGA
                     GDYLTATLRTSEVAALVIIFVEASMGLFLMEALRITHLFPRIATLNDHMRRRMMWIAL
                     ILLVTLAGVEAALALMRDMLIADKQALIQSLATVQQAATTDGWVGRIPTAGQMLLGFI
                     LPFALAFIAIPLESLIHSSRTVGGVLLAVLVSTLALTLRVLANVSRRMSRVLITLYDV
                     VIVLPLLAERMTKAGMAMRHSAVDADDSAHQAR"
     gene            complement(17667..18368)
                     /locus_tag="VIG70_13260"
     CDS             complement(17667..18368)
                     /locus_tag="VIG70_13260"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8251568.1"
                     /translation="MKKFGVREVEKLLRLPRRTIHGLIKAGFVSPSRGPRRAYLFSFQ
                     DLIVLRTAQALAAANVPARRITRSLKELRRHLPDSMPLSGLSIGAVGERVVVKEGASR
                     WQAESGQYLLAFEGDPVAGDLKVMEKTESIEALVERGYFLHEAGRLAQAEAVYRSALE
                     TWGADALLLYNLGVLLEDLGRKAEAMESYQAALRGDPLMADCHYNLALLCEQLSRPQE
                     AIRHMARYRKLVAEK"
     gene            complement(18386..19225)
                     /locus_tag="VIG70_13265"
     CDS             complement(18386..19225)
                     /locus_tag="VIG70_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY8251569.1"
                     /translation="MAARSIATLTISFGMVSIPVKLYSATEASRAMSFNLLHKACGSR
                     LKQQYFCVKEEVPVAREDMAKGYEFAKDQYVMFTPEELKALEEAGTHTAEITEFVPVK
                     AIDPVFFDKAYYLGPDKGGAKPYALLARALKESGRCALGRWAARGKQYIVLIRPIDTG
                     LVEGLVMQQLLYAAEVRSINDVDIPKTEVKPAELRLAQQLIEQQSADKFDPAAYTDEV
                     RARVEAAVQKKVEGQEITMAEAPQQGAQVIDLMEALKASLEKKPAKKVEAAKPATRKA
                     AKK"
     gene            complement(19293..22001)
                     /gene="ligD"
                     /locus_tag="VIG70_13270"
     CDS             complement(19293..22001)
                     /gene="ligD"
                     /locus_tag="VIG70_13270"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEY8251570.1"
                     /translation="MAKGKLTDKRKLSDYAAKRAFDATPEPAAAVPEGRSGPLLFVIQ
                     QHSATRLHYDFRLECDGVLKSWAVPKGPSLDPADKRLAVPTEDHPFDYASFEGVIPPG
                     QYGAGEVIVWDCGVYSPDEGAAWVEDRRAAEKQVLDGLEKGKLSVTLRGEKVKGSFAL
                     VRTSRGKDWLLIKHKDRFVAKEDVGARNRSVLSGIAVDEMKAMPVQRMAASNLVPGGK
                     REVMPDRLEPMHAELAGAPFNKPDWMWEPKLDGYRVLAFLKDDKVSLRSRRGLELAAT
                     FPGVAAAIAEQGVNMILDGEIVAFDENGKPSFNALQNRFQLKTGRDIAAAEKSTPVVF
                     YAFDLLHFAGVDLRGAPYADRRRYLAQCLLPSALVQLVHAADDGVELYAAALKSGFEG
                     VIGKRKQSKYEPGKRSASWIKVKSTSSAEFVVGGYTKGKGARATLGALLVGYWQGKRL
                     RYASHVGSGFDGRTLKQVQALLEPLRRKSSPFDEKPELNAPTTWVEPQVAVEVKFQGW
                     TDDGSLRAPVFLRLRDDVDAKSVRRTDPETAVPKPDAGSEIDEILSQLERCKASGTIS
                     IGAEVLKLSNLDRIYWPADPALKQPALTKRDLLRYLAQVSPFMLPHLADRPLTMIRMP
                     DGINGQRFFQKHWKQARPKFVQAITVFSGSKDESHEYLVCNNLPTLLWLAQSGTLEFH
                     VWHSRARPGEDCMAQDSDYSSSLDALESSVLNYPDYVVFDIDPYIYSGKEAPGDEPEL
                     NTVAFEKGKEVAFRLRELLNGMSLDPIVKTSGKTGLHVFLPIRRTIDFDAAREVSEKV
                     GRHLMRLHPKDITLEWSVPKRTGKIFMDYNMNVRGKTLNVAYSPRGAPGAPVSMPLTW
                     DELAKAHPLDFRLTNAAEFLAGSEDRWRDALVRKQSLERALGRAKA"
     gene            22000..22713
                     /locus_tag="VIG70_13275"
     CDS             22000..22713
                     /locus_tag="VIG70_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY8251571.1"
                     /translation="MPNSRKLLAGASGYSFKEWKGSFYPESIKPDAMLSYYAERLPTV
                     EINNTFYQMPKVAVLENWARLTPESFKFAIKASRRITHMSRLKADSAADSVGFLYRNL
                     ASLGAKRGPVLYQLPPFLKKDLPRLTEFLGLLPEGHGAAFEFRNDTWFADDVYDALRS
                     AGAALCLSEREDNAPPPLVETAAWGYVRLRLETYSDRDLEEWARRLGATGWGEIYVYF
                     MHEPTAPAYAQTLMKLAAV"
     gene            complement(22710..23144)
                     /locus_tag="VIG70_13280"
     CDS             complement(22710..23144)
                     /locus_tag="VIG70_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol oxidase"
                     /protein_id="HEY8251572.1"
                     /translation="MRNFVAVLGAMGLLLAAGCQSTSEEKPPAQEKKPAQVVKVDQDG
                     VQRIAIVAGSYFFKPSHIVVKVNVPVELVASRESGMTPHDLLIQAQDAGIVVQQDLAT
                     EPRKIAFTPKKVGKYAIYCSKKPPLMASHRERGMEGILEVVP"
     gene            23238..24200
                     /locus_tag="VIG70_13285"
     CDS             23238..24200
                     /locus_tag="VIG70_13285"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8251573.1"
                     /translation="MHRAALALLALLFLPAVAAAQTWPAKPVRMLVPFPPGGGVDFAA
                     RIVGKHLADRLGQSVIIENRAGANGIIALEALKSAAPDGYTIAAASNGPLVINPFLYL
                     KLPYDPVRDFVAIGRLVDFPLLLVTHPSLPAGSVAELIALAKAKPGVLTYSSPGVGNG
                     SHLAVELFASMANVSLVHVPYKGTAPAATALLAGEVSLTFSSIPTVLPHIRAGKLRAL
                     AVGNAARLPALPEFATVAESGVPGYEAFSWAGLIAPAGTPKEVVVRLNRETAQILGQK
                     DIVDQLARDGTLAAPDAPEEFSAYIKAELDKWAAIVKLAKIKPE"
     gene            complement(24205..24642)
                     /locus_tag="VIG70_13290"
     CDS             complement(24205..24642)
                     /locus_tag="VIG70_13290"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEY8251574.1"
                     /translation="MKRASIAGHPVHPALVHFPVALWSVTLAWDLLGLWTGAGVWWQT
                     GFWCLVAGTIMAVPAAIAGAMDFAALEERHPAEGAALRHMLLMGSAFTAYVGAVIVRA
                     GPQVPEGWRLYAALSLSAAGFALLAAGGWYGGRLVYHYGVGRD"
     gene            complement(24650..25006)
                     /locus_tag="VIG70_13295"
     CDS             complement(24650..25006)
                     /locus_tag="VIG70_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251575.1"
                     /translation="MITWAAIVFAIAALGGATLATMHFRGRTPLPMPLALLHGLLAAT
                     GLVLLIAAALTMPGFGGMALAALVIFLIAALGGFYLFAHHLRGRPLPSAVVLIHGSAA
                     VVAFLLLLGFLFNVKV"
     gene            25149..25793
                     /locus_tag="VIG70_13300"
     CDS             25149..25793
                     /locus_tag="VIG70_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8251576.1"
                     /translation="MHTAPAVAAASSPDTELARRVVAGDHAAFAELMRRYNRLLYRTA
                     RAILKDDAEAEDALQDAYLLAFRAIANFRGEAKLSTWFVRIVANEALGRLRKRARAAE
                     VTYLDETARDEPERAALRTEVMRLLESKIDRLPDAFRTVFMLRALEEMSVEETAAALD
                     IPEATVRSRYFRAKGLVREALASEIDAAFEDAFGFAGARCDRMVASVMARIAAL"
     gene            complement(25768..26664)
                     /locus_tag="VIG70_13305"
     CDS             complement(25768..26664)
                     /locus_tag="VIG70_13305"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8251577.1"
                     /translation="MRKDYSTTRTLGVVGLGIMGGAMAEALLASGYSVCGYDPAAAAA
                     RRLKRAGGLPLASAAAVAERAGVLITSLPSSGALEDVVNQIQDPRARILVEMSTLPLA
                     DKERARRRLARAGVAALDCPVSGTAVRLKERTWTIFASGDKRAFARVRPVLKVLADAI
                     PYVGAYGNGTRMKLVANHLVAILNVASAEAIGFARGMGLNPREVLEIFGPSPVVGTGV
                     LRLRGELMVKRRYKPPTMKIGVWQKDMRIIGEMARSVRAATPLFDACGPVFDAALARG
                     YGDADTASVYEVLSARGRRSLP"
     gene            26663..27730
                     /locus_tag="VIG70_13310"
     CDS             26663..27730
                     /locus_tag="VIG70_13310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251578.1"
                     /translation="MASAALEVVDVEVSYGATPVLKRISLSVAAGEFVALLGPSGCGK
                     TTLLRAVSGFVPVNAGRISVGGRDITQAPPDKRGMAMVFQSYALWPHMTTAQNLGYGL
                     KLRRVAKPDIAKRVAAILAMLKLEGFGERNVTQLSGGQRQRVALGRALAISPELLLLD
                     EPLSNLDARIREEVRHEIKALQAKLGITTIHVTHDREEAMVMADRIAILDAGRIAQLG
                     SPEEVYNRPNSPFIASFMGAGNVLELEARPAGGGIEIQAGAHNDGIFVPGSSLAGPIK
                     AHFRSEAARLGPADARAADRLLLRGRIVQASYPGGFYRYAVAVGQNQFMVDDTRRLAV
                     GEMVGIGLPLSSLHFFSRREQ"
     gene            27727..>28492
                     /locus_tag="VIG70_13315"
     CDS             27727..>28492
                     /locus_tag="VIG70_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8251579.1"
                     /translation="MKLHLALAALTAALAATPAAAQKINLNVVTAGDQNMVDYVKDFL
                     APQFEKNNPNATVKAVGTGPGDAGSQKIAEKLQAQKSQSAWDVDVAVIHQKMAGDLVR
                     EGLLAPYVRDVSTGRLVTSEASKNALGTNVTGYVIPMFQSQTAIAWNPALVKAPPKNY
                     DELAAWAKANPRKFGYNGIKGGMSGVAFVVGWVYANTPDSDLLMKGPYDAATKARWDG
                     ALGKLKEFNRSVVITPGNAGTLDMLNRGEIAMGPVWV"
BASE COUNT         4586 a         9663 c         9380 g         4863 t
ORIGIN      
        1 cgatcgccgc cccgccttgc agcgtgaggt tgcccgcgct gatcgtcgtg gtgccggtgt
       61 aggtgttggt ccccgacagc gtcagcgtgc ccgcgccgtc cttggtgagc gtccccgtgg
      121 tcgtggcgat cgccccgctc tgggtggtgt cgcccgcgcc gcccaccgtc aggttctgcg
      181 cgccgctgat gccgccggtc agcgtcagca ggttcgcgtc ggagttaatc cgcgcggtgc
      241 ccgccagcgt gatcaccccg ccaaaggagt tgttgccgct cacgttgcgc agcgcccctc
      301 cggcagggcc ggcgccggtg atcgtcagcg cctcggcgcc gaccgcaatg ctgccctgca
      361 actccagggc cgcgccgctc gccaccgtcg tgccgccggc ggtcgcgccc agcgcattgg
      421 cgtgctggat gttcaacact ccgacgtcaa tcgccgtcgt gcccgcgtag gtgttgttgc
      481 ccgtcagcgt gaccgtgctc gcgccttgct tggtgagccc cgccgagccg ctgatcgtgg
      541 ctgcgatcgt ggtgtcggtc gcatcgccga aagtgagcgt cgcatccagc accacgctcg
      601 tgccgccggc cccggagagg cgcccgatgg tctcggccgc atcgatctcc agcgtcccgc
      661 cgttgagcgc gaccactccg gcatcggcga tcgccgcccc gccctgcagc gtgaggttgc
      721 ccgcgctgac ggtcgtggcg ccggtgtagg tgttggtccc cgacagcgta agcgtgccgg
      781 ccccggcctt ggtcaggccg ccggcgcccg tgatcactgc gccagccgct tgactgctcg
      841 cgcccgagac gttgatggtg agcaatcctc ccgcgtcgat agttccggcc gcttgcgaga
      901 aggttgaaga cgcgacggtc agggcgctgc tggtcagcgt gatgtcgtcg ttcagcgcaa
      961 cgttgccggt cgcggccgtg aggttcaacc caccggcgac cgtccgggtg atcgtcgcac
     1021 cggtgctttg aacgaggtcg ccctgcgcaa tgatcgagag gttgtttcca ttgttgagcg
     1081 ttcctgcccc aaggatggtg atgtttccgg cttcgccggc gtcgaatccg gtagtggttc
     1141 gaacgctgac gtcgccgccg ccaagcgctg catcgatttc gtcccagccg atggtcgagc
     1201 ccgaagcggt cgccggctcg aatggagtaa cagtggtgac gttcgtcgac gccgtaccgg
     1261 caacgatggt caggtcggtc gggtcgagca gaagcattcc cgccttgccg ttcggggcgc
     1321 ggcgatcgac cgatccttga aagtcgaggt ggccattgcc cgaaacctcg gcgaatccgc
     1381 cattgccgct tgcggcgccg ccgcgcacgg agccggtgcc gtagaaacgc gttgttccgt
     1441 cggcccagac gattatcgtg ccgccattcc cggagcggag ggcgtccgcc atgatgcgcg
     1501 ctttggtgcc gacgtaggtc atcgacgcgt tcgcctcagg cccggcgccc tgataattcc
     1561 cgccgatgag gaccgtgccg ccgcccgcgt cgcctgaagc atcgatccgg gccttgtcaa
     1621 agagccccac gtacttgccg agcactttaa ccgtcccgcc ggtcgttccc gcatccgctc
     1681 cggctgcctc cagcctgccg ttgccgctca cggccacgac gccggcgctg ccgccgtcca
     1741 gcacgatctc gccattccgc tcgacgagcg agttggcgcg gatgattccg gagttgttga
     1801 tgacggtgtc gagcagcgcg ttggcgctgc gtgcgctgag aaaaactctg ccgccgtcgg
     1861 cttcgatgct gccggtgttg acgaccgagg cgttgaccac ggcctgctcg acattgatgc
     1921 tgatcagacc gtcgccgacg aggtcgagag agacgcggtc ggccgcggcg agcgccaccg
     1981 tgccggcgcg agcaacgatg atgccgtcgt tgcgcacctg gggcccgacc agcgcggtat
     2041 agccgttggt cgtgacgatg cgcccttcgt tgaccacgct gcccgccgtt ccctcccggg
     2101 accattgata gcggccgttc ttgaagttct cgtcggtgat ggaaagcgtc gtggcgaaga
     2161 gtgcgccgac ttcgaccgac gcggtcctgc cgaacagcac cccgttggag ttggacagga
     2221 agacctgccc attggccgtc agggtgccga aaatgttcgt cggattgccg ctgatatccc
     2281 gcaccagaaa caccgagttt ctgccgagct ggtggatgtt gaccgtctcg ggcgccgcga
     2341 tggaaaagcc gccgcggtag ctggtcgccg cgtgctgggt cgttacgtcg atccgcatcg
     2401 tgtgcgacgt gggctgcgaa tggaccaccg tgccgacggt ggtgagcggg tcgccgggaa
     2461 gcgcgtgggc agagccccag gggagcagcg ccgcgctcac cgcgagagcg gtggcgcgcg
     2521 gcctgaggtt caatggcgct tgcgtttccg ctttgatgcg cttcgttgct gccatggcct
     2581 tccccttaca acctcaccat gaaacggcgc gtcggccagt gtaggccggc ccactttcgc
     2641 ccgttgtcag gaaccgcaat atgcggaatt aggtcacgca tcctcagaac cacttcaccg
     2701 cctggatcca cacgcgcggg ttgcggctct ccgggtcggc ggtcggcact tcgtccccga
     2761 cgcgccagga cgcgctcgca cgcacgatga attcgccctc ccggccaagc gatgccccga
     2821 cgccgtatcc ggagatcgag cgtttgttct tcgtttccgt cggcagcggc ttctcgttca
     2881 cgtcgacctg tccccagtcg tagagcgcca tcaccacgac gtcgccctcg aacaccttga
     2941 cgcccggcca gatgtagcgc acctcggact ggaccagatt gccgctgtcg ccgttcgcct
     3001 cgcccaccgg ataggcgcga acgccgttcg ctccgccgag cgacatcttc tcggcggacg
     3061 ccaggttctt ggaggcctgt tgtccggtga gcgcgagcag aacgctcagg ttgtcggtca
     3121 cccggttcag gcgcttgaaa tcgtagttcc acttgcggaa cgtgccgacg gtgttcaggc
     3181 ccgtcaccgc ggcgtccgct tgggccagcg cagtcggcgc gagcttgagg tcgccttgcg
     3241 tgtaggtcac agagaatgcg ttcaagccgc cgctgaggaa gcggtcgcgg aagtcgccga
     3301 cgacgcccgc cttgatcgag tcgatgaagc ggtcctccac cgtggcggtg ctatcgatgc
     3361 ggtcgaacag gcgcttgtcc tcgtaagcga actggccgat caggttcgtg ttgcgcgtgc
     3421 ggtagagggg gtggaacgcg aacacggtct tcaccaggcc gtcgccgttc gcctcgaggg
     3481 aggcaaactc gtcgccaagc ttgtagctga acttggtgac gctcgcgccg atgcgcgtgc
     3541 cccagaagcc tgccggtacg atgtaggcga tgcgcccgaa atacatctcc tcgtcggtgg
     3601 cgaaggcgcg cagcgagagc tggtcgccga tggtgagcgg attgttcacg ttgaggctgc
     3661 cgcccagccg gtacgcgccg gtgaagcggc tgccctggtt gtcgaaatcg acgtagccgt
     3721 tgaggaggcc cacgccctgg tcgacgttca cccgcaggtc cgcggcgccg acggtctggc
     3781 tcgggcggat ctcggaagtc accgccgccg tcggcaagtc gttgatgagg agcagcggct
     3841 tctcgaggcc ggtctcggtg atgatgtcgc cggccttgag gtgcgcattc aggattccgg
     3901 cgagcagcca ctcggcgagg cgcgtggccg ggcggcgctg cagctcgacg atgccgacgc
     3961 gaccctcgat gacggcgatc tcgaccacgc cgtcgcggat cgcctgctgg ggcagatagg
     4021 cctgggcgag gaagtagccg cgctcgcggt ggtaggcgcg caccttggtc gcggcgtcgg
     4081 tcagaccctc gaagtcgagc tccttgccga cgaactcctc caccacctgg gcgagcgtcg
     4141 cgtcttcgta cagcgtgttg ccggagaagg tgaacttgct gatacggact ttgagcgtcg
     4201 gcgaaaccgg cagcgcgggg cgcggctcgg gcggccgcgg tctgatatcg tcatccggcg
     4261 gcggcggcag gcgcagcggc tggcgctgct gctcgaggat ctgtccggcg tcagggcgct
     4321 gctgcgcgat cgcgaccgcg ctcgcgagcg aaagcaccgc gacgacgatg ggcctcgcga
     4381 tcagcccggc gttgcgcaaa cgcgcatcgc tctccctgga gaggcgaacc ctcatttccc
     4441 tgactcccgg atttcttgtt ggtgccgatg tcacgcgtgc acggcatgtg cgagcgcgaa
     4501 acgcggcgtt tctggaccgg caccgggagg gcaagattcc ccgaccgcga gcgtgacgtg
     4561 ccgacaaaag ccggcgtgcg gcaagctcat ggctcccctt accccagtgc atcaactttt
     4621 ttcctaattc agcgggttac cgcttgttct tgtctttcgc ctgttgccaa gcgtgcgcgc
     4681 ggagaatagc gtgatttatt ttgtgcagca acgaatcaac tgtgatttct tgtgtattga
     4741 cgcggtgcgc cattgcgcgt cgtcgtcgcc cgacgaaaag ataagttaag gacgtcgcgg
     4801 ccccggaaca aattacaaga cgagaacgta cgaagctctt tctaacgtgc gcggtcatcg
     4861 cggcgggtct cgccgggcgc tcggcgctcg acaagatcct cgcgctgcgc ggcggcgcgg
     4921 agctcgtcgc gctgtgggcg caactctcgt cgctcatcga gatggtcggg gcggtcgcgt
     4981 cgagcggcgt cggcgcgggt ctctccgtgc tggtcgcgca gacgcggctg cgcgagcggc
     5041 agcagctttt cctgcgtcgc gcgctctatc tcgggctggc gatctccgcc ccggtgggcg
     5101 tcgctacggc gatcgtcggc tggaatctgt ccgagcgcct tggcggtgcc gcgctcgcgc
     5161 cgcagagcgt cgtgctcgcg gcgctgtgcg gctggatcgc ggtgatccac gggctcgtca
     5221 acagcttctg gctcgggcag cagcgccgcg acctgatgct ggccctcgcc ttgctggccg
     5281 ccgcgctcgc gctcgcggcg gcggcgcttg cgccgcccgc gttcgtcctg gagctgatca
     5341 tcgtcgcgca agcggccccg gcggccgtgc tgctcttcgt gccgcaccgc gccgccgcgc
     5401 ccacgcgcgt cgaagaccat gcgctcgagc gctacgtcct tcccggcgtg gtcatcggca
     5461 tcctcagccc ggcatcgatg atcgtcgcgc gcagcctggt cgccgacgcg ctttcctggc
     5521 acgagagcgg cgtgctgcag gcgctctggc gcgtctccga ctggatttgc ggcttcgctt
     5581 ccggcgtgct tagcgtgctg tatttaccgc gcttcgccgc tgcttacccg caggcgcggc
     5641 ttgcgtcggt gctgcgcgaa tccgcgcgca ccgtgctgct gccctcggcg gtgctcttcc
     5701 tgctgctgtt cgccttctat tctccggttc tcgaagcgct ctacgacccg agcttccggg
     5761 cagcgccgct cgcggtgggg ctcctattcg ccggcagcct ggcgcgcatc gcagcctgga
     5821 tcccgctttt cgggctatac gcggcaacgc gcacgcgcgc gatcgcggtg ggagagcttc
     5881 tgtcacttcc ccttttcgcc gcgctcacct acgcggccgg agatgggctg acgctcgaga
     5941 tcgtcggcgc gctgtggctc gcgagctacc tcgcctacgg ggcgttcaac ttctgggcgc
     6001 tgcggaaggc gtgaccccga acacgaacag gttggcgacc acgatggtcg ccgccaagaa
     6061 atagaaggtc gcgagcagcc cgaagcggtc ggccacgatt ccccccagga gcggcccgat
     6121 cgcgcctccg agcgcctgcg cgccgaacag cacgccgatg ctcgacccac ccatgttttt
     6181 cggcgtcgat tcgagcatcc aggcctggat caccgggcgc gtcgcgtaaa ggaagaaccc
     6241 gagcacggcg accagcgcga tgaagagcgg cgagccgccg ctgaacgcca ttgccgccag
     6301 cacgaccgcg gtcgcggcca tgctgctcac caggatctgc ttgcggccca ttcgatcgga
     6361 gagatgcccg gcgatcggag aggcggcaaa tccggccgct tgcagcgcga acaggcaggc
     6421 gccgacccag aacggcgaat agcccatgtc gtgcgccagg tagaccggca ggaaggtgag
     6481 cagcgtcgtt tgcgtcatgg tacggaacgc cgagcccgtc gagagcagca ccagcgcacg
     6541 gctcgccaga aggtctttga gcccggcgag gtagtgcgtg aacgactgcg cttcgtgctc
     6601 ggatttcttc gccccgagcc gcatgctgcc gagaaacacg aagagcagcg ccgcgacgac
     6661 gagcccgggc accacgttca gcacgaccgc ttcgcgccag gagaaatacg tcaacgccgc
     6721 accgatgatc accggcgcga ccgcgtcgcc gacgtttccg cccatgccgt gcaccgagag
     6781 gaccaggcct ttgcgctcgg gatagcgccg gccgagcgta gggatcgccg tcggatgcca
     6841 caggctgttg ccgaacccga cgagcgcgac gcacgcaagc agcatcgcat agctatgcgt
     6901 gaaaccgatg agaaggtagg gaaaaccgat ccagaaaagc gacaccgcca tcagcaatcc
     6961 cttgcgcccc acggtgtcga ccagcacgcc gcccggcacg ttcgcaatcg cggatgcgat
     7021 gtactggcag gtcatgatga gcccgatctg gctgtacgac agcccgagct cgctgccgat
     7081 gagcggcagc aggaggtaga aggtcgccgg ataccagtgc gtcagcccgt gaccgatggt
     7141 gatcagccag agctcgcgcc atgcgttgcg ggataccggg tggggtggaa ggacggccgc
     7201 gctcaaggcg cacagtatac tggcgcggca aacgatggcc gtttaccctg tggtgctgct
     7261 gtccgtgctg acctcgatcg gcttgcgcgg cagcaaggtc gcggtctcgc tctacgcgct
     7321 cgagctcggc gccggcgccg ccgcggtcgg cgcgctcgca gcgctgttct cggtgcttcc
     7381 cttgctgctc gcggtaccgg cggggcgcat cgccgaccga ttcggcatgc gtatgccgat
     7441 cgtgctgggc accgcaatca tggccggcgg ccttgccctg ccgttggcgg cgcctggcct
     7501 gggttggctc ttcctctgtc ccacgctcat cggcctggga catgttttct ttcacgtctc
     7561 ggtgcataac ctcgtgggct cgctcggcga cggcgcggcg cgcgcgcgca acttcgccac
     7621 gttcgccctc ggcatttcga tcgcggcttt tatcggacct tcggccgcgg ggtttgccat
     7681 cgagctcgcc ggcttccagg cagcattcgc gctccttgcc tgcatcgccg ccgtgccggc
     7741 tttctacgcg ctgttccacc ggagcctctt tcccccgcgg cagcgccttg aaggcgagca
     7801 cgccgctgga agcgctttcg agctgctctc gatccccgca ttgcgccgca cgctgatcct
     7861 gagcggcgtc gccctaaccg gaatagagct gttctcgttc tacctgccga tctatggacg
     7921 cgcgatcggc ctggcgcctt ccacgatcgg catgattctc tcgagctacg cgctggcagg
     7981 gttcgtcgtg cgcatcgtca tgcaccgcct cacccaacgc tacggcgagc tcggggtgct
     8041 gtccggctcg ctgttcgccg ccgccggcgc ctacttggcg gtgcccgctc tctcggatgc
     8101 ctggctcctc gccggagctg cgtttgccct cggcctggcg ctcggctcgg cgcagccgct
     8161 caccatcgcc ctcacctatg gctacgcgcc gcccgggcgc tcgggcgaag cgctcggcat
     8221 gcgcatcatg gcgaacaaga tcactcagat cgccgtgccg cttgctttcg gcggcttcgg
     8281 cgccgtcctg ggcgccgcgc cggtgtttct cgccacgggc gcgttcctgc tggctgctgg
     8341 ctgcctgtcg gtccgcgggg cgggtagtag acttgcccgc atccaggaga cccgttcgtg
     8401 agagcggacc agaacgagct gctcacccgc accgcggctg gcacgcccat gggcgagctc
     8461 ttccgccgct actggctgcc ggcgctgctc gcctccgagc tgcccacgcc cgcagggccg
     8521 ccggtgcgcg tcacgctcct gtcggagccc ctggtcgcct tccgcgacag cgcgaaccgc
     8581 ctcggcctga tcgacgaatt ctgcgcgcat cgcggcgcgt cgctctggtt cggccgcaac
     8641 gaggaaggcg gcttgcgctg cgcctatcac ggctggaaat acgatgccgg cggccagtgc
     8701 gtcgaaatcc cgtcggagcc ggacaacccg aagctctgtc gccgcatgaa gctgcggtcg
     8761 tatccgctca tcgagcgcgg cggcgtgctc tggacctata tggggccgcg cgagcggcag
     8821 ccgccgccgc cggagcacga atgggccatg gtcccggacg ctcaccgctt cgtctcgaag
     8881 cgcttgcagg agtgcaacta cctgcaggcc atggagggcg gcatcgactc gagccacgtc
     8941 tcgttcctgc accgtcacac gatcacgacc gatccgctgt tcaggggatc gaagggcaac
     9001 gagtacaacc tgcaggatct gcggccgcac ttcgaggtcg tcgagtcgcc cggcgggctc
     9061 tacatcggcg cccgccgcgg cacgggcgat ggccagtggt actggcgcat cacgcaatgg
     9121 gtgatgcctt ctttcacgct gattccgccg cgcggcgacc atccgatcgg cggacactgc
     9181 tgggtgccga tggatgacgt ccattgctgg gtctggagca gcaatcacca cgctacgcgc
     9241 gcgctcaccc ccgaggagcg agccgcgctc gaggccggca aaggcattca cgccccgctg
     9301 attcccggca ccttccgccc cgtcgcgaac aaggacaacg attacctcat cgaccgccgg
     9361 gcgcaggagg aaggacgcag cttctccggt gtcgagggct tcgccatgca ggacgcctcg
     9421 gtgcaggaaa gcatgggacg ggtgcaggat cgcacccgcg agaacctcgt gcccaccgac
     9481 ctgggcatcg tgatggcgcg gcgccgcctg attgccgcgt caaagctcgt gcagaagggg
     9541 aagagtcccc ctggagccga cccgcgccac cagcgcgtgc gctcggtggc aatcgtcttg
     9601 ccgcaaggag tggcgttcag cgaagcggct ggaggagcgc tggtcccgac accgggatcg
     9661 gctcacacca cagtctgacg cgcggcccgg cggtagctgc gccagagcgg cagcaccagc
     9721 gaaagcacca ggaaacccca cagcacgttg ccgagcgtcg acgagaacag gatcgtcagg
     9781 tcgccctgcg aaatgcgcaa ggcgcgcgcg agatactgct cgaacagcgg gccgaggatg
     9841 atgccgatca gcatcgcggt gacgtggtag ttcgtcttgt gcgcgatgta cccgatgacg
     9901 ccgaagacca gcgcgaggcc catatcgaac aggtactggc gctcgacgaa cgccgcgacc
     9961 acggtgacca ggatgatgat cggcgcgagg taggtggtct cgatgagcac gatgcgcgcc
    10021 atgtagcgcg caagcggcag gatggtgaag atcatgaaga tgtaggcgac cgccatggcg
    10081 acgaacacac cgtaggcgag ctccgggttg gtgctgaaga gccgcggccc catcaccatg
    10141 ccgtggtact gcagcacgat catcatcacc gcggaggtgg tgccgcccgg gacgccgagc
    10201 gccatcatgg gcaccagagt tccggaggtg acgccgttgt tcgccgcctc gggcgcgatc
    10261 acgccctcgg gaacgccagt gccgtagagc tccggatgtt tcgaccacat ccgcgactgc
    10321 tggtaggcga cgaaggccgc gatgctcgcg ccggcgccgg gcacgatgcc gatgaacagg
    10381 cccacgaacg acgtccagac gatgtgccac cagtgcacga ccgtgaggcg gagtccgtcg
    10441 agcgtgtcgg cccagctcgc gcgcatcgcg cgctcgcgcc cttcggagct gacgatcagc
    10501 ttttcctcca gcatgaccag cgcttccgac accgcgaaca gaccgatcag cgcggcgacc
    10561 agcggcacgc cgtcgtagag ctcgaggaag ccgaaggttg cgcgcggcgc gttgtagatg
    10621 tggtcggtgc cgatgacgcc gatcagcagg ccgaacaggc ccgcgatcag gcccttcagc
    10681 gtgtccttgg cggcgatcac tgcgatcagc gtgatgccga acagcatgac caccaccatc
    10741 tcgaccgagc gcaggtagag cccgatctgc gacaaccagg gcagcagcgc gagcgtagcc
    10801 agcgtggtga tcagcccgcc gaacaccgag gcggtaaagg acagcacgat cgcctgctgc
    10861 gcctggcctt tctgcgtcat cggatagccg tcgagcgtgg tggccgctgc gcccccggag
    10921 ccgggaatct tgatcaggat ggcgggcaca ccgccggcca ggtggttcgc gcagtacagc
    10981 gagaccatga aggcgaacgc ctgctcgact ggaatgccga gcgtcagagg cagcagcatg
    11041 atcaacgtgt tctgcggact gaacccgggc aggatgccga ccacgatgcc gagcaggatg
    11101 ccgagccaga taatccacca cagcgcgaac tgcgccgcga actgcccagc gatgaactcc
    11161 cagctcaaga aaagattttc tcgatgaaac cgtgtgggaa gccggcgtcg agcacggcaa
    11221 taaacagcag gtaagccgcc gcggccaccc caaaggagat gccgaagatg agcgacggcc
    11281 tgcgcacgtc catgagccag agcgccaccg ccatcgacgc gaacagcgac agcgtcaggc
    11341 caaggacctg gagcaacaag atgaacgcaa cggtgaccgc caccatgccg atgcgcttaa
    11401 ggagaacgtc gcgcggctgc cagagcggat cggtgcgcag gtcgccccgc ccctgcgcca
    11461 cctcgagcgc gatgcgcgcg acctggacca ccaccagcgc gatgagcgcc gtgccgatga
    11521 ccacgccgtt cgccttcgcc tcccaggcga gatcggtgat cgaccagaag aagtagcccg
    11581 cgaagccgag cgcgagcgcg gggataacga ggtcggcgcc gagcggcgcc ttccgcgaca
    11641 ccctcaaccc ttcttcttgg cggtcagcag cgaccgatac tcgttcgcca gctcgaccat
    11701 ccccttcgcg tagcgggtgc acaccgtgcg gtcgccgtac tggatggcct ccaccggcgc
    11761 gccggtcttc gcgaaggcct ctttgaacgc cgggtcgttg accacctgct tcgaggtctt
    11821 ttcgagctgc gcgaagcgct ccggatactt ctcgatcgcc tcggtatgga tcgcccagga
    11881 gcgcgacgac gaaagctcgg gcagcttggt gccgaagacc ttgttgatcg gcggcgcgtt
    11941 gtcgctctgc gcggcgagcg ggttctcgtc gttgaacact gcgagcacct tcagctgctc
    12001 gcgctgggcg atgatgccgg cgatcggcag cgccccgcag tcggcctcgc cgttcagcac
    12061 cgccaccgtg gtggggttgc cgccgccgta agggatcagg ttgaacctag cgccgagcgc
    12121 cgcgccaagc gccagcatgc cgatggacgc cgggtgcggc aggcggctca cggccacgtt
    12181 gaccgggcgc ttcttcgcct cggccaccag atcctcaacg cgctggaact tgcccttgcg
    12241 cagcgaaaac accccgctgt cgtcgatgtc agtgcggcag aagtactgga agtcctcggg
    12301 gaagcgatag ttcggcttct gcagcgcgta catgatcatc tccggcccca tgttgccgaa
    12361 gagcaggttg tagccgtcct tctcgcgttt gtgaacgaag gtttcgtaac cgacctggcc
    12421 ggctgcgccg gcgtagaagc tgtactcgaa gttggtcttc aggagcttgc tccagacttc
    12481 gtcgaaggag cgggcgagcc gctcggcgcc gcccccctgg ccggtcggga tcacgacgcg
    12541 gatgttcttt gacgggaagc cctgcgcccg agcgcccagc gggcccagcg cgagtgcagc
    12601 gctagcccct gcgagaagac gtcgcctgct ctggttggcc tgtttcatcg atgcatcctc
    12661 ctaggaatgc ggatattaga atgccgcgcg gctaggaagg aatagaactt tgaaacgatg
    12721 gctcgcggtc gctctgcttg cctggggtcc cgcggccctg gcgcaatgga acgagctctc
    12781 cttcgtcgat ccctcgctgc gctggcgtac cctcgaaacc gcgaatttcg cggtccactt
    12841 tgcggagcag catcgtgcgc aggcgcgcac ggtggccggc acggcggaga ggatcctccc
    12901 cggcatgacc gccctgctgc gatggaggcc ggaatcgcgc atccacgtag tagtgctgga
    12961 ttccgccgat ttcgccaacg ggctcgcttc gcccctgccg ttcaactaca cgatggtgtt
    13021 tctctcgccc ccggacgagg gcgagctcct gcagaaccgc gaatggctcg agctggtgct
    13081 gacgcacgag ctcttccacc tcgtgcatct cgacatggcg cgcgccgcgc cgctcggcct
    13141 gcggcgcgtt ttcggacgcg tgccgtttct cttccctaac ctgctgcagc cgcgctgggt
    13201 cgtagagggg atggcagtgc acgccgagtc cgaccctgcc cgcggttacg ggcgccttgg
    13261 ccaggcgcac tttgaaggca tgatgcgcgc cgaggcggcg cggggcttgc gctcgctcga
    13321 ggagatcaac gccggcggcc gcggctttcc gctcaaccgc gactacctgt acggcgggta
    13381 cttcttcgcg tttctgcagg agcgctacgg ccgcgagggg ctcaccgcgt tcatcgagaa
    13441 ctacagcgac aacatcatcc ctttcagggt gcacagcaac ccggtcgcgg caaccgggaa
    13501 aaacatggat cagctctggg ccgagtatca caactggctg accgcgcgct tctccccgaa
    13561 ggtggcttcg gcaaggcaag gcgaggtcat tgcccaaggc tggtccatta cgagccccgc
    13621 gctcaccccc gatggcacgc gctggtacat cagcggaagc ggctacacgt tgccgaggct
    13681 cgttcgccag gcgcccggcg gcaagccgca agcgctgcgc gatgtggagc tcgacgcgcg
    13741 cctgtcggct tccccgggcg gcggcctgct ccttgctcaa ccggagatct gcgggaacta
    13801 caactactac tacgacttca accgagtcgg tcccgacggc agcctgaagc gcctcaccga
    13861 atgcggccgc taccgcatcg gtgcgcccct ggacgacggc cgcaccgtgg gggtccgcac
    13921 ggagaacggg ctcgcgcagg tcgtgacgct cgaaggcgag gttctctacc gggcggctgc
    13981 gggcgaatcg atcactgggg tcgctgccag cggcgcgtcg gtcttcgtga cccgccttcg
    14041 cgatgggacg tggtcattga tccgcatcgc cgggcagaac acggaagtcc tgctttcgga
    14101 tagcgcgatc aagcattcgc cccgcctcga tgccgccggg gaggtgttct tcattgccga
    14161 ctacggcaag gttttcaacg tctggtcgct gcgcggcggc cgcctggcgc gctggaccga
    14221 ggcggcgcac ggcgtgcgcg agatgagcgc tccgcacggc ggcgagctct tgctgaccac
    14281 gatcgaagcg gagggcgaca cgctgcgcac gtaccggctg cctgattcgc ctttgcaaga
    14341 catgagcatt ccggcgtcgg agccttcccc gtcgagcacc atgccgtcga ccgaagcacc
    14401 cgatcgcgct tattcgccgt ggagctcggt gctgccgagg tcctggctgc cgctcatcga
    14461 gatcgccgac ggcgcggtca agctcggcgt ggtgactttc ggccaggacg cgctcgggct
    14521 gcaccagtac gcggtaacgc cggtgatcga gttgacgcag caggagctgc tgggcaacgc
    14581 gctctatgtc tatgacggac ggcaccagct gctcctggac cgcacgatgc gcgtcaggga
    14641 aacggtggac gacgaggtcg aggcctacac catcgaagaa ggtgcgcaat gggtatccac
    14701 ctggcggcac ctctccctca accgccgctt ctactggggg ctgggcggcg cgctcgagcg
    14761 cgagcggctg caccgggcgg gcggcccgac gctttccgag caggacgagc gcgtgctggg
    14821 gctcgtggca gggctggaca cgcgtcgcgc gcagtggctg tcggagggcc cgagccaggg
    14881 catgcaggtg cggctattcg ccgagacctc gcacggcctg catgcggcgt attcgggcga
    14941 tgtctaccgg gtggattggc ggctgcactt cccgctcggc cgcacggtcg tgtcgctgcg
    15001 ctggaacgag gcctggggcg agcccgatgc ggaaccgttc cagctcggcg gcagcgattc
    15061 cgacccgccg accctgctgc cgattctcaa ccagcgggat ttcgcgctgc gcgggtacgg
    15121 cagcggcgag ccttctctcc tggggcaccg cgcgcgccta ggcacgctcg agtggcgcgt
    15181 gccgctgaag gacctcgatc gccacggcat ggtgccgccg gtcggattca accgcttcgc
    15241 gatgaacctt ttcttcgacg tcggcgatgc ctggcggcgc ggcgccgagc cggactacca
    15301 ccgcggcttc ggcatcgagc tgatgtccga gatccggatt ggctatgtgt tcggctacga
    15361 cttccgcctc ggggtcgcga aaggtcgcga ccagggcggc gagaccaccg cctacctcag
    15421 gctcggacga tcgttctagc ttctggagaa gatcgcgtcc aggcgctcga ggtcgttgat
    15481 cacgcgccac tccgacgcgc ccgcggccac gacgcgcttc tgccaggcct cgaggcggcc
    15541 gaaatagcgc cgcggatcga tgttgtcggt cttgagcttc acgacattca ccgccaccac
    15601 gcggatgcgc ttgagattca tcgggatatc gcgcacggtg atcttgtcga tctcttcttg
    15661 catgtcggag aacacgacaa tggtcttggt gccggcgccg gtctcgttga ggaactctgc
    15721 cccctgcagc aggccgccgg tgatatcggt atagcggctc cccttcacgt tcttgaggaa
    15781 gccgtcggtc ttctcgcgaa acgcgcgctt ttgcgcgttg gcctgcgagg gacgcgcgtc
    15841 gaaggtcgcc ttggcgatga tgtccttctc ggtgaagctg cggctttcga cccgcgccac
    15901 cgcgagcgaa tcgcccggct gcagcgtgcc gagcaggtag ttgacgatca cttgggccct
    15961 gccggcctcc tgggagtagg tcccggaagt gtcgatcagc acatagacgg cctgtgcatg
    16021 gctgcgcgaa tcgccgcagc ccgccgagaa gctgccggcc agcagcgcca acgacagcag
    16081 caatagtcgc ttgaacatgc ttacctcgct tggtgcgccg aatcgtcggc atcgaccgca
    16141 gagtgtcgca tcgccatgcc ggctttcgtc atgcgctcgg ccaacagcgg cagcacgatg
    16201 acgacgtcgt agagcgtgat cagcacccgg gacatgcgac gcgaaacgtt cgcaagcact
    16261 cgcagcgtga gcgccagcgt cgacaccagc acggcgagca gcacgccgcc gacagtgcgc
    16321 gaagagtgga tcagcgactc gagcgggatg gcgatgaacg ctagcgcgaa cggcaggatg
    16381 aagccgagca gcatctgccc ggcggtcggg atccgcccga cccagccgtc ggtggtcgcc
    16441 gcctgctgca ccgtggcgag cgactggatc agcgcctgct tgtcggcgat cagcatgtcg
    16501 cgcatcagcg cgagcgccgc ttcgacgccc gccagcgtca ccagcaggat aagcgcgatc
    16561 cacatcatgc gccggcgcat gtgatcgttg agcgtggcga tgcgcgggaa gagatgagtg
    16621 atgcgcagcg cttccatcag gaacaggccc atcgacgcct cgacgaagat gatcaccagc
    16681 gcggcgactt ccgaggtgcg cagggtggcg gtgaggtagt cgccggcgcc gaccatctcg
    16741 gacatcggca gcgcgatcag cttgaagttg atgaacgcgc cgcctgcggc caccgccatc
    16801 accagcagcg cgatcgcgaa ctgggtgaac gccgatatcg tcagtacgtg ctccgccttg
    16861 tcggtcttcg cgttgatggc ctcgtacttc gccatcaggc cgtcgacgcc cttggcgctc
    16921 tcttgcaggt cgccgagctt cttgttgacc tcgctcatgt tgcggtcgac cgagcgccag
    16981 aacggcatga agctctcgag gatcttgtgc cgggtctcgt aggagcggcg gtattcgccg
    17041 atcgccttgt cgtgaatctt ggcgatcgag gccttgatct cctcgaggat gcgctggacc
    17101 atctcgctgc cgccggtctt gacgttggcc atcgcagtga cggcctcgac ccagtcgggt
    17161 ggcggcggcg gcacttcgcc gcacttcttg tagtcctcct cgatgcgggt gatctcgtcg
    17221 agcagcttcc tctgcagcgt cggatagccc tgcaagtcgc gcctcaccac cgcgccaagg
    17281 cgctcgaact cgcgctcgat gcgttgcccg acctcgagac gcccgtgcgc cagcagcacg
    17341 gcccggttgc gctccttcat ctgctccgcg gtggcgatga gccaacgcgc ccccagcctg
    17401 agcgggccgc cgatcaagtg gccgacggcg cgcagcaact cgtgcaccgg cttgcgcgca
    17461 gcgtagagaa aaacgagcgc cacgaccgct acgacgaaga tcgagagcgc gggactgtcg
    17521 ggccagagca tccagaatga cgtttcggtc atgacgttct ccttctccgc cagagcctag
    17581 agcaggaatg ccgcacgctg gagactaact cgcgcccttc aggcattaag tctcgaatgc
    17641 tgcgggcgcg tcgtgaactt tgcgcgttac ttttccgcca cgagttttcg atagcgcgcc
    17701 atgtgacgga ttgcttcctg cggccggctg agctgctcgc aaagcagcgc gaggttgtag
    17761 tggcaatcgg ccatcagggg gtcgccgcgc agcgccgcct ggtaggactc catcgcctcg
    17821 gctttgcggc cgaggtcttc cagcaggacg cccaggttgt agagcagaag cgcatcggcg
    17881 ccccaggtct cgagcgcgct gcgatacacc gcctcggcct gcgccagccg tcccgcctcg
    17941 tgcaggaaat agccgcgctc gaccagcgcc tcgatgctct cggtcttctc catgactttc
    18001 aggtcgcccg cgaccggatc gccctcgaac gcgagcaggt attgtccgga ctcggcctgc
    18061 cagcggcttg caccctcctt gaccaccacg cgctcgccca cggccccgat ggagaggccc
    18121 gagagcggca tcgaatccgg caggtgtcgg cgcagctcct tcagggagcg ggtgatgcgc
    18181 ctggcgggca cgttggccgc agccagggcc tgcgcggtgc gcaggacgat caggtcctgg
    18241 aacgaaaaga gatacgcccg gcgcgggccg cgcgacgggg acacgaagcc cgccttgata
    18301 aggccatgga ttgtcctgcg cggcaggcgc aggagcttct ccacctcccg caccccgaac
    18361 tttttcacgt tctacgtctt cagttctact tcttcgcggc cttgcgcgtt gcgggcttgg
    18421 cggcctcgac cttcttcgcg ggcttcttct ccaggctcgc cttgagcgcc tccatgagat
    18481 cgatcacctg cgcgccctgc tgcggcgcct ccgccatggt gatctcctgg ccctcgacct
    18541 tcttctgcac cgcggcctcg acgcgcgcgc gcacctcgtc ggtataggcg gcggggtcga
    18601 acttgtcggc ggactgctgc tcgatcagct gctgcgcgag cctgagctcg gccggcttga
    18661 cctcggtctt cggaatgtcg acgtcgttga tcgagcgcac ttcggcggcg tagagcagtt
    18721 gctgcatgac caacccctcg accagccccg tgtcgatcgg gcggatcagc acgatgtact
    18781 gcttgccgcg cgccgcccag cggccgagcg cgcagcggcc cgattccttc agcgcgcggg
    18841 caagcagcgc gtacggcttg gcgccgccct tgtcgggccc gaggtagtac gccttgtcga
    18901 agaacaccgg gtcgatcgcc ttgaccggaa cgaactccgt gatctccgca gtatgcgtcc
    18961 ccgcctcttc gagcgccttc agctcctcgg gcgtgaacat cacgtactgg tccttggcga
    19021 actcgtaccc cttggccatg tcttcgcgcg cgaccggcac ctcctccttg acgcagaagt
    19081 actgctgctt gagccgcgag ccgcaggcct tgtgcaggag attgaacgac atcgcgcggc
    19141 tcgcctcggt cgccgagtag agcttgaccg ggatggaaac catcccgaag gagatggtca
    19201 acgtggcaat cgatcgtgcg gccatgcttt cacctcttac aatcaacagg ttacgacatt
    19261 aacttcgagc gaagggttcg aagcatactc cattatgcct ttgcgcgtcc caatgcccgc
    19321 tcgaggctct gcttgcgcac cagcgcatcg cgccagcggt cctccgaccc ggcgaggaat
    19381 tcggccgcgt tcgtaagccg gaagtcgagc gggtgcgcct tggcgagctc gtcccacgtc
    19441 agcggcatcg acaccggcgc gccgggcgct ccacgcggcg agtaggccac attcagggtc
    19501 ttgccgcgca cgttcatgtt gtagtccatg aagatctttc cggtgcgctt cggcacgctc
    19561 cactccaggg tgatgtcctt gggatgcaag cgcatcaggt ggcggccgac tttctcgctc
    19621 acctcccggg cggcatcgaa gtcgatggtg cgccggatcg gcaggaacac gtgcaatccg
    19681 gtcttgccgg aagtcttgac gatcggatcg agcgacatgc cgttcaggag ctcgcgcagc
    19741 ctgaaggcga cttccttgcc cttctcgaat gccaccgtat tcagctcggg ctcgtcgccg
    19801 ggcgcctcct ttcccgagta gatgtatgga tcgatgtcga ataccacgta gtccgggtag
    19861 ttcaataccg agctttcgag cgcgtccaga gaagaggaat agtccgaatc ctgcgccatg
    19921 caatcctccc cggggcgggc gcgcgaatgc cagacgtgga actcgagcgt gccggattgc
    19981 gcaagccaga gcagcgtcgg caggttgttg cacaccaggt actcgtggct ctcgtccttg
    20041 gaccccgaga acacggtgat cgcctggacg aacttcgggc gcgcctgttt ccagtgcttc
    20101 tgaaagaagc gctggccgtt gatgccgtcg ggcatgcgga tcatggtgag cggccggtcc
    20161 gcgaggtgcg gcagcatgaa gggcgacacc tgcgcgaggt aacgcagcag gtcgcgcttg
    20221 gtgagcgccg gctgcttgag cgccgggtcg gcgggccagt agatccgatc gaggttgctc
    20281 agcttgagca cctcggcgcc gatcgagatc gtgccgctcg ccttgcatct ctcgagctga
    20341 ctcaggatct cgtcgatttc cgacccggcg tccggcttgg gaacggccgt ctccgggtca
    20401 gtgcggcgca cgctcttcgc atcgacgtca tcgcgcagcc gcagaaaaac cggcgcgcgc
    20461 agcgagccgt cgtcggtcca gccctggaac ttcacctcga cggcgacctg cggctcgacc
    20521 caggtcgtgg gcgcattcag ctccggtttc tcgtcaaagg ggctggactt acgccgcaac
    20581 ggctccaata gtgcctgtac ctgcttcaaa gtcctgccat cgaaccccga gccgacatgg
    20641 gaggcatagc gcagcctctt cccctgccag tagccgacca gcagcgcgcc gagcgtcgcg
    20701 cgcgccccct tgcccttcgt gtagccgccg accacgaatt ccgcgctgct cgtcgacttg
    20761 accttgatcc atgacgccga gcgcttgccg ggctcgtact tgctctgctt gcgcttgccg
    20821 atcacgccct cgaagccgct cttcagcgcg gcggcgtaga gctcgacgcc gtcgtcggcc
    20881 gcgtgcacca gctggaccag ggccgagggc aacaggcatt gggccaggta gcgccgccgg
    20941 tctgcgtagg gcgctccgcg caggtccacg ccggcgaagt gcagcaggtc gaatgcatag
    21001 aagaccacgg gggtgctctt ctctgccgcc gcgatgtcgc gcccggtctt cagctggaag
    21061 cggttctgca gcgcgttgaa agaaggcttg ccgttctcgt cgaaggcgac gatctcgccg
    21121 tcgaggatca tgttgacgcc ctgttccgcg atcgccgcgg ccacgcccgg gaatgtcgcg
    21181 gcgagctcga ggccacgccg cgaccggagg gaaaccttgt cgtccttgag aaaagcgagg
    21241 acgcgatagc catcgagctt cggctcccac atccagtcgg gcttgttgaa cggcgcgccg
    21301 gcgagctcgg catgcatcgg ctcgagccgg tccggcatga cctcgcgctt gccgccggga
    21361 acgaggttcg acgcagccat gcgctgcacc ggcatggcct tcatctcgtc gacggcaatc
    21421 ccggacaaca ccgaccggtt ccgcgcaccg acgtcctcct tcgcgacgaa gcggtctttg
    21481 tgcttgatga ggagccagtc cttgccgcgc gaggtgcgca cgagcgcgaa ggagcccttg
    21541 accttctcgc cccgcagcgt gacgctgagc ttgcctttct ccaggccgtc gagaacctgc
    21601 ttttctgcgg ccctgcggtc ctcgacccag gcagcgcctt catcggggga atagaccccg
    21661 cagtcccaga cgatgacctc acccgcgccg tactgccccg gcgggatcac gccttcgaac
    21721 gaggcatagt cgaaaggatg atcctcggtc ggcaccgcca ggcgcttgtc ggccgggtcg
    21781 agcgacggcc ctttaggtac ggcccaggac ttgagcaccc cgtcgcactc gagacgaaaa
    21841 tcgtaatgca agcgcgtcgc ggaatgctgc tggatgacga aaagcagcgg cccgctgcgc
    21901 ccttcgggca ccgccgcggc cggctcgggc gtggcatcga aagcgcgctt cgccgcatag
    21961 tcgctaagct tccttttgtc agtaagcttc cctttcgcca tgcccaattc taggaaactg
    22021 ctcgccggcg cgagcggcta ttcgttcaag gagtggaagg gtagcttcta cccggaaagc
    22081 atcaagcccg acgccatgct ctcctactac gcggagaggc tgcccaccgt ggagatcaac
    22141 aacacgttct accagatgcc gaaggtggcg gttctggaga actgggcaag gctgacgccc
    22201 gagtcgttca agttcgccat caaggcttcg cggcgcatca cccacatgtc gcgcctcaag
    22261 gccgacagcg cggccgactc ggtgggcttt ctttatcgca accttgcgag ccttggcgcg
    22321 aagcgcggcc cggtgctcta ccagctgccg ccgttcctca agaaagacct gccgcggctg
    22381 accgaatttc tcggcttgct gcccgagggc cacggcgcag cctttgagtt caggaacgac
    22441 acctggttcg ccgacgatgt ctacgacgcg ctcaggagcg cgggcgcagc gctatgcctt
    22501 tcggagcgag aggacaacgc cccgccgcct ctcgtcgaga ctgccgcgtg gggctacgtg
    22561 cgtcttcggc tcgaaaccta ttccgaccgc gatctcgagg aatgggcgcg caggctgggc
    22621 gcgaccggct ggggcgaaat ctacgtgtac ttcatgcacg agccgacggc acccgcgtac
    22681 gcgcaaacgc tgatgaagct cgcggcggtc taaggcacca cctcgaggat cccttccatc
    22741 ccccgttcgc ggtggctcgc catcaagggc ggcttcttgc tgcagtagat ggcgtacttg
    22801 ccgaccttct tgggcgtgaa cgcgatcttg cgcggctcgg tggcgagatc ctgctggacg
    22861 acgatacccg cgtcctgcgc ctggatgagg aggtcatgcg gcgtcatccc ggattcccgg
    22921 ctcgccacga gctctaccgg cacgtttacc ttcacgacga tgtggctcgg cttgaagaag
    22981 tagcttccgg cgacgatcgc tatccgctgg acgccgtcct ggtcgacctt gaccacctgt
    23041 gccggcttct tctcctgggc gggcggcttc tcttcgctcg tggactggca acccgccgcc
    23101 agcagcaagc ccatcgctcc aagcaccgca acgaagttgc gcattggctg atcctttcgc
    23161 atgttcctat cgcaaacctc cataattatt ggtggagggc cgatggtcaa tcgcgagatc
    23221 cagataggag caaactcatg caccgcgcag cgctagcgct tctcgccctg ttgttcctcc
    23281 cggctgtcgc ggcggcccag acctggccgg ccaagccggt gaggatgctc gtcccgttcc
    23341 cgcccggcgg cggcgtggac ttcgccgcgc gcatcgtcgg caagcacctc gccgaccggc
    23401 tcgggcagtc ggtgatcatc gaaaaccgcg cgggcgcgaa cggcatcatc gcgctcgagg
    23461 ccctgaagag cgccgcgccc gacggctata cgatcgccgc cgcgtcgaac gggccgctgg
    23521 tgatcaatcc gttcctctac ctcaagctgc cgtacgaccc ggtgcgcgat ttcgtcgcga
    23581 tcggccgcct ggtggacttc ccgctgctcc tcgtcaccca tccttcgctg ccggcaggca
    23641 gcgtcgccga gctcatcgcg ctggccaaag ccaagccggg cgtgctgacc tactcgtctc
    23701 ccggcgtcgg caacggcagc cacctcgcgg tagagctttt cgcgtcgatg gcgaacgtgt
    23761 ccctcgtcca tgtgccctac aaagggacgg cgcccgccgc gaccgcgctg ctcgcgggcg
    23821 aggtgtcgct caccttcagc agcatcccca ccgtgctgcc gcacatccgc gccggaaagc
    23881 tgcgcgcgct ggcggtgggc aacgcggcgc gtctgcccgc gctgccggag tttgcgaccg
    23941 tggccgagag cggcgtgccg ggctacgaag cgttttcctg ggcgggcctg atcgcgccgg
    24001 ccggcacccc gaaggaagtg gtggtgcgcc tgaaccgcga gaccgcgcag atcctcgggc
    24061 agaaggacat cgtcgatcag ctggcgcgcg acggaaccct cgccgccccg gatgcgccgg
    24121 aggaattcag cgcctacatc aaggcggagc tggacaagtg ggccgcgatc gtgaagctcg
    24181 cgaagatcaa gccggagtag ccggctagtc gcgtccgacg ccgtagtggt acaccaagcg
    24241 gccgccgtac cagccgcccg cagcgagcag cgcgaagccc gcagccgaga gcgagagcgc
    24301 ggcgtacagg cgccaaccct cggggacttg cggcccggcg cgcacgatca ccgcgccgac
    24361 gtaagccgtg aaggccgacc ccatgagcag catgtgacgc agcgccgcac cctccgccgg
    24421 atgccgttcc tcgagcgcgg caaagtccat tgcgccggcg atcgcggcgg gcacggccat
    24481 gatcgtccct gccacgaggc accagaagcc tgtctgccac cagaccccgg cgccggtcca
    24541 gaggccgagc aggtcccacg ccagggtcac cgaccacagc gcgaccggaa aatgcaccag
    24601 cgcaggatgg accgggtggc cggcgatgct cgcccgcttc aagcccggcc tagaccttca
    24661 cattgaagag gaagccgagc agcagcagga aggccacgac cgccgcgctg ccgtgaatga
    24721 gcaccacagc cgaaggtaac ggcctgccgc gcaggtgatg cgcgaacaga taaaaccctc
    24781 cgagcgcggc gatgaggaag atcacgagcg ccgcaagcgc catcccgccg aagccgggca
    24841 tggtgagcgc cgccgcaatc agcagcacca ggccggtcgc ggcgagcagg ccgtgcagca
    24901 gcgcgagcgg catcggcagc ggcgtgcgtc cccggaaatg catcgtcgcg agcgtggcgc
    24961 cgcccaacgc ggcgatcgcg aacacgattg cggcccatgt gatcatttcg tcgtctcccc
    25021 tgtggtggta ggtgctgaaa attagcgcaa tttccggaaa gctggcgaag gtttttttcg
    25081 ggtcgccggg aacgcaccgg ccccgcgagt catccaatgc gcagcatccc tcgtatcagg
    25141 agatcaccat gcacaccgcg cctgccgttg cggcagcctc ttcgcccgat accgagctcg
    25201 cgcggcgggt ggtcgccggc gaccacgcgg cgttcgccga gctgatgcgc cgctacaacc
    25261 gccttcttta ccggacggcg cgtgccatcc tgaaagacga tgcagaggcc gaggacgccc
    25321 tgcaggacgc ctacctgctc gccttccgcg ccatcgcgaa ctttcgcggc gaagccaagc
    25381 tctcgacctg gttcgtgcgc atcgtcgcca acgaggcgct tggacgcctg cgcaagcgcg
    25441 cgcgcgcggc ggaggtgacc tacctcgacg agaccgcgcg cgacgagccc gagcgcgccg
    25501 cgcttcgcac cgaggtcatg cgcctgctcg aatccaagat cgaccggttg cccgacgcgt
    25561 ttcgcaccgt gttcatgctg cgcgcgctgg aggagatgtc ggtcgaggaa actgccgccg
    25621 cgctcgacat tcccgaagcg acggtgcgct cgcgctactt ccgcgcgaag ggcctggtgc
    25681 gcgaagcgct ggcgagcgag atcgacgccg ccttcgagga cgctttcggc ttcgccgggg
    25741 cgcgctgcga tcgcatggtg gcctcggtca tggcaaggat cgccgccctc tagcggaaag
    25801 aacctcgtag accgaggcgg tatcggcgtc gccgtagccc cgcgccaaag ctgcgtcgaa
    25861 caccggcccg caggcgtcga agagcggcgt cgccgcgcgc accgagcgcg ccatctcgcc
    25921 gataatccgc atgtccttct gccagacgcc gatcttcatc gtcggcggct tgtagcggcg
    25981 cttcaccatg agctcgccgc gcaggcgcag cacgccggtg ccgaccaccg ggctcgggcc
    26041 gaatatctcc agcacctcgc gcggattcag ccccattccg cgggcgaagc cgatcgcctc
    26101 ggcggacgcc acattgagaa tcgccaccag gtggttcgcg accagcttca tgcgcgtgcc
    26161 gttgccgtag gcgccgacgt agggaatggc gtcggcgagc accttcagga ccggccgcac
    26221 ccgcgcgaat gcgcgcttgt cgccgctcgc aaagatcgtc caggtgcgtt ccttcagccg
    26281 taccgcggtg ccgctgaccg ggcaatcgag cgcggcgacc cccgccctgg caagccggcg
    26341 ccgcgcgcgc tccttgtccg ccagcggcag cgtgctcatt tcaacgagaa tgcgtgcccg
    26401 tggatcctgg atctggttca caacgtcctc gagagcgcca gagctgggga gcgaagtgat
    26461 caagacgccg gcgcgctcgg cgacggctgc ggccgaagcc agaggcaggc cgccggcgcg
    26521 cttcagccgc cgcgccgccg cagccgccgg gtcgtaaccg cagactgaat atcccgaggc
    26581 gagcagcgcc tcggccatgg cgccgcccat gatgccgagc ccaacgacgc ccagcgtcct
    26641 cgttgtggaa taatcttttc gcatggcgag cgccgcactt gaggtggtcg atgtggaagt
    26701 gtcgtacggc gccacgccgg tgctcaagcg gatttcgctc tcggtggcgg ccggcgagtt
    26761 cgtcgccctc ctcggcccct cgggctgcgg caagaccacc ctgttgcgcg cggtctcggg
    26821 cttcgtgccc gtcaacgccg ggcgcatctc ggtcggcggg cgcgacatca cgcaggcgcc
    26881 gcccgacaag cgcggcatgg cgatggtgtt ccagtcctac gcgctctggc cccacatgac
    26941 cacggcgcag aacctcggct acggcctcaa gctgcgccgg gtcgccaagc ccgacatcgc
    27001 caaacgcgtc gccgcgatcc tggcgatgct caagctcgag ggcttcggcg agcgcaacgt
    27061 gacccagctc tccggcgggc agcgccagcg tgtggcgctc ggccgcgcgc tcgccatcag
    27121 cccggagctc ctcctcctgg acgagccgct ttccaacctc gacgcgcgca tccgcgaaga
    27181 ggtgcgccac gagatcaagg cgctgcaggc gaagctcggc atcacgacga tccacgtcac
    27241 ccatgaccgc gaggaagcga tggtgatggc cgatcgcatc gccatcctcg acgccggccg
    27301 catcgcccag ctcggctcgc ctgaagaggt ctacaaccgg ccgaattcac cgttcattgc
    27361 ctccttcatg ggcgccggca acgtgctcga gctcgaggcg cgccccgccg gcggggggat
    27421 cgaaatccag gccggcgctc acaatgacgg catcttcgtt ccagggtcct ccctggctgg
    27481 ccccatcaag gcgcatttcc gcagcgaagc ggcccggctc gggcctgccg atgcccgggc
    27541 ggccgaccgg ctgttgctgc ggggccgcat cgttcaggcc tcctatccgg gaggttttta
    27601 ccgctacgcg gtcgcggtgg ggcagaatca gttcatggtg gacgataccc ggcggctcgc
    27661 ggtgggcgag atggtgggaa tcgggctgcc gctatcttcg ctgcatttct tttcgaggag
    27721 ggaacaatga aactgcacct tgcgctggca gcgctcacgg cggctctggc cgcgacgccg
    27781 gcggcggccc agaagatcaa tctcaacgtg gtcaccgccg gcgaccagaa catggtcgac
    27841 tacgtgaagg actttctcgc tccgcagttc gagaagaaca atccgaacgc gacggtgaag
    27901 gccgtgggca ccgggcccgg cgacgcgggc tcgcagaaaa tcgccgagaa gcttcaggcg
    27961 cagaagagcc agtcggcctg ggatgtcgac gtggccgtga tccaccagaa gatggccggc
    28021 gacctggtgc gcgagggact gctcgcgccg tatgtgcgcg acgtgtcgac cggcaggctc
    28081 gtgacctccg aagcctccaa gaacgcgctc ggcaccaacg tcaccggcta cgtgatcccg
    28141 atgttccaga gccagacggc gatcgcctgg aaccccgcgc tggtgaaggc gccgcccaag
    28201 aactacgacg agctcgcggc ctgggccaag gcgaacccgc ggaagttcgg ctacaacggc
    28261 atcaagggcg gcatgtcggg ggtcgcgttc gtggtcggct gggtgtacgc caacacgccg
    28321 gattccgacc tcttgatgaa agggccctac gacgcggcaa ccaaggcgag gtgggacggc
    28381 gcgctcggca agctcaagga gttcaaccgc agcgtcgtga tcaccccggg caacgccggc
    28441 acgttggaca tgctgaaccg cggcgagatc gccatggggc cggtgtgggt cg
//
LOCUS       DATBSZ010000207         8706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1811315, whole genome shotgun sequence.
ACCESSION   DATBSZ010000207 DATBSZ010000000
VERSION     DATBSZ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8706
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811315"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..1941)
                     /locus_tag="VIG70_13320"
     CDS             complement(271..1941)
                     /locus_tag="VIG70_13320"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8251580.1"
                     /translation="MCTLCDQGKPQDHSRKPQRSQLGRRDFLKASTATAAAAAGASLF
                     AANRARAHDDDDAPQDSGRRGRRYVIRGGSVMTMDPNMPNKGEWPKADVLIEGKKILA
                     VGPNLHVWGAGEIDARGKIVMPGFVDTHHHQAWTAIRSSIPDSILIDDKSGTPSAAQN
                     YFGNILLGFAPRYRPEDVYISELFGGLAQLDNGVTTVHDVSQIHHSPDHSDEAIRALF
                     HTERRAAFGFFESAGASVLGTNPDNQYPQDATRIMQRWFSSSDQLVHMIMGGEVYLGD
                     ASTVASWTLGRQLGLQVAAHILSPFGIRSILDRLAGLNGGFPDGKGGFIDIRDDNLFI
                     HMTGMSDEAWDVVRNKGAQVSVAFPIEMNMRHGIPPIDKLQRRQMEPSLSSDVETNLT
                     ADFFTQMRSAMTMQRMMVNQEILNTGDFTNFFFEAVPGSAWPTVPVPDGNQPLLNVRD
                     VLRYATINGAKHLRLDSKTGSLTPGKEADIIILDGTHINVFPVNHVPGAVVQMMERSN
                     VETVIVAGKVRKWKGKLLDVNLGRLRRELEKSRDSIYAAAGVPQNLFR"
     gene            complement(2168..2833)
                     /locus_tag="VIG70_13325"
     CDS             complement(2168..2833)
                     /locus_tag="VIG70_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251581.1"
                     /translation="MNKNKIVVLLLVLAAGCGTTLQDGAYPPPMDPTRKVSEQDCSTL
                     FDTGGGNLMCRQVTEAERRAKIAEEERQAKARREAEERAARERLERERAEQLALEEAA
                     RKQRAAEEEAARKLRAAEEERLAAERRRLEAEAALLAQAERERQERAEAEARARQRAE
                     QEAREAAERECRRLEQERSDGRALLANFVANYGKDEEFKEIAGQITAFLDKPRKVKPC
                     PPA"
     gene            3219..4991
                     /locus_tag="VIG70_13330"
     CDS             3219..4991
                     /locus_tag="VIG70_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251582.1"
                     /translation="MSSRTPQVLLVLALAAVLPPAWAHGDEARQCRARNDSIPTSLGA
                     DGWAAVDGPVTGGCAAGRTRVFHVYDRRQLVLALNNDLLLLLEHLFPRLDVKRRHLLD
                     HRSKIIYIHRTIDLNVDHRNRPLVEEDYMRMCGYTAHDTYYDPATGNQDGSGGFFGAY
                     KAEYDPNRWIRQSLDTADNRPPALSGPLEEARLCFQRAQAERVMIDVGSNTSIIGVGP
                     DAKIINGGLRIGFMNQFDPNDRDNYRAENVVVRNITFADAFDMFPGWDPKDSFSITIT
                     NTDGCQAAFDEATDSGPHRCPTRRGGRWNTEYDGISVMNAKHVWIDHNTFTDEPRFDT
                     QFPPVFGAPFNEPTQKTAHHDGHVDVTLLATKVTISNNHFLRHDKGNLLGGSDVANLV
                     PGYGPGKIDVTFHGNYYQNMIQRMPRVRFGRVHVYNNVFDANRLANAEYRLGDTWATG
                     TAAKLVTENNLFDILNNNLTIPRIINYASTVANFDLCVSAGFTAAECGTYYYDTGTWI
                     TMNRVTGTPPTATTLFDAFAAVKAIQESSAANAPLFKLDPADPAVFWYPSQTYSYLLA
                     PVSTETERTELRTTVINNAGAGKL"
     gene            5259..5423
                     /locus_tag="VIG70_13335"
                     /pseudo
     CDS             5259..5423
                     /locus_tag="VIG70_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            5502..5915
                     /gene="gspG"
                     /locus_tag="VIG70_13340"
     CDS             5502..5915
                     /gene="gspG"
                     /locus_tag="VIG70_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEY8251583.1"
                     /translation="MRRSRVKGFTLLELLVVVVIIGLLAGFVAPRYFSQVGKSEVNVA
                     KAQIDALEKALDQYRLDVGRYPSNELGLKALVDKPTSEPKWNGPYLKKQVPLDPWGKA
                     YIYKMPGEKGDFDLLSYGRDGAAGGSGEDADITNR"
     gene            5922..7103
                     /locus_tag="VIG70_13345"
     CDS             5922..7103
                     /locus_tag="VIG70_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8251584.1"
                     /translation="MRFHLRAITNEGRIESLDYQAVDEQSARQQAESRGYTVLQVQSR
                     RSVSLLRQSRFPVQLFSQELLVLLSAGLPLVDAIETLGEKEKRDEWRALLGSLVAVLR
                     QGQPFSVALEQQPAVFSQLYVATVRAAEKTSDLAPALSRYVAYQAQIEAIRKRALNAS
                     IYPMVLIGAGAVVSLFLLFYVVPRFGKIYEDRTTELPFFSKILIGWGQVVEGHAMIAL
                     GLLAGLVIAIIFAARDPRAKAFLGDILWRVPAIGERLKVYQLARLYRTTGMLLRGGMP
                     LVSALGMSAELLHPVLRERLAATSKAISEGLGVSESFDKNGLATPVAMRMLVVGEQGG
                     NMGEMLDRAAGFHDEELARWVDWFTRMFEPLLMALIGLVIGAIVILMYMPIFELAGNL
                     R"
     gene            7100..>8706
                     /locus_tag="VIG70_13350"
     CDS             7100..>8706
                     /locus_tag="VIG70_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY8251585.1"
                     /translation="MIEAKTIAEARAEAHAATGSRRVVDVLEEKLALQPDEFAAKLAA
                     TFRLAVLRMDELRRLEPAFDLVPLSECGRKACVLLRAEGGELLMATDDPFSAEQVAWA
                     AERIRAPFAWRLVHRGDLVAYLASHEETLRALDAVRADVGVAGDENVGAEDLSLKAIG
                     EEKSEVVRLVRSTLRDALKIGASDIHLETVAAGLVIKFRIDGILIQIKVIEDRAQAEQ
                     AIARIKVLAELDVTERRVPQDGRFKAIDRLRAIDFRVSIMPSIHGEDAVLRVLDKQSL
                     YESTRQQLSLDSLGFEEADIKALRRLSHEPHGMLLVTGPTGSGKTTTLYAAITEINNG
                     QDKIVTIEDPVEYQLPGVLQIPVNEKKGLTFARGLRSILRHDPDRIMVGEIRDPETAN
                     IAVQSALTGHLVFTTVHANNVFDVIGRFMHMNVDLYGFVSACNAILAQRLVRMVCPHC
                     AEAFAPDKALLEESRLTDQQVQGWQFKVGKGCRECRGAGYRGRRAIAELLILNDELRE
                     MIIGRSPIRALKEAATRNGTRFLREAAL"
BASE COUNT         1455 a         2915 c         2858 g         1478 t
ORIGIN      
        1 ccgcccaccg cttcacaggc atccctagcc ccgagaaagc gggaggctat cgcctgaggg
       61 gcgctaccaa gctcggcgct ttgctcttcg cagatggttc tggaagcgca tcggtggcgt
      121 ccccacgggg attcgaaccc cggttaccgc cgtgaaaggg cgatgcccac gctttcccgg
      181 tttcgatgta gatcgtcaag ggcacgaatt tcgtggcctc aaaaaactgc gggcggccgg
      241 agccgcccgc ggtgttgcga caggagtggc ctaccggaac aggttctgcg gcacgcccgc
      301 tgcggcgtag atggagtcgc gagacttctc aagctcgcgg cgcagcctcc ccaggttcac
      361 gtcgagcagc ttgcccttcc acttgcgcac cttgccggcg acgatcaccg tctcgacatt
      421 gctgcgctcc atcatctgca cgacagcacc cggtacgtgg ttgaccggga acacgttgat
      481 gtgcgtgccg tcgaggatga tgatgtcggc ctccttgccg ggcgtgagcg agccggtctt
      541 gctgtcgagc ctcaggtgct tcgcgccgtt aatggtggcg tagcgcagca cgtcgcgcac
      601 attgaggagc ggctgattcc cgtcgggcac gggcaccgtc ggccacgcgc tgcctggcac
      661 ggcctcgaag aagaagttcg tgaagtctcc cgtattgagg atttcctggt tgaccatcat
      721 gcgctgcatg gtcatggcgg aacgcatctg cgtgaagaag tccgccgtca ggttggtctc
      781 gacgtccgag ctgagcgacg gctccatctg ccgtctctgg agcttgtcga tgggcggaat
      841 gccgtgccgc atgttcatct cgatcgggaa ggcgaccgag acctgggcgc ctttgttcct
      901 gaccacatcc caggcttcgt ccgacatccc cgtcatgtgg atgaacaggt tgtcgtcccg
      961 gatgtcgata aatcctccct tgccatcggg gaacccgcca ttgagaccgg cgagacgatc
     1021 caggatcgag cggatcccga acggcgacag gatgtgggcc gcaacctgca aaccgagctg
     1081 gcgcccgagt gtccaggacg ccaccgtgga agcgtcgccg agatagactt cgccgcccat
     1141 gatcatgtgg acgagctgat cgctcgagga gaaccagcgc tgcatgatgc gggtggcgtc
     1201 ttgcggatac tggttgtcgg gattggtccc aagaacgctg gcgccggcgc tctcaaagaa
     1261 gccgaaggcg gcgcggcgct cggtatggaa cagggccctg atggcctcgt ccgagtgatc
     1321 tggcgagtga tgtatctgcg aaacgtcgtg caccgtcgtg acgccgttgt cgagctgcgc
     1381 caggcctccg aacaactcgc tgatgtacac gtcctccggc cggtaccggg gggcgaaccc
     1441 gagcaggatg ttgccgaaat agttctgagc cgcgctcggc gtgcccgatt tgtcgtcgat
     1501 caggatgctg tcaggtatgg agcttctgat cgccgtccaa gcctggtggt ggtgggtgtc
     1561 gacgaagccg ggcatgacga tcttgccgcg cgcgtcgatc tctcctgcgc cccagacgtg
     1621 caggttcggg ccgacagcca gtatcttctt gccttcgata agcacatccg ccttgggcca
     1681 ctcgcccttg ttgggcatgt tggggtccat tgtcatcacc gagccgccgc ggatcacgta
     1741 gcgccgcccg cgccggccgc tgtcctgcgg cgcatcatcg tcgtcgtgcg ccctggcgcg
     1801 gttcgcggcg aacaggctcg cgcccgccgc ggctgccgcg gtcgccgtcg aagctttcag
     1861 gaaatcgcgc cgcccgagct gcgagcgttg cggcttgcga gagtggtcct gcggtttacc
     1921 ctggtcgcac agcgtgcaca tggttcccct cctctagggt cgagcgtgcc gtagtactaa
     1981 tgtactaacg ctttttccgg ctcgttattc attctcgccc caagccgcag cggaagtaaa
     2041 tgcgatcgtc gcgcgcggtg ggccgggcag tgggacgcct gcgagcatcg gcgagtgggc
     2101 aagctctggc cgcggcgaga cgacagtcga agatcagggc atcaggcgag cgcggcccgt
     2161 tgtgccttca cgctggcggg catggtttca ccttgcgagg cttgtcgagg aacgcggtga
     2221 tctgcccggc gatctccttg aattcctcgt ccttgccgta gttggcgacg aagttcgcga
     2281 gtagggcgcg gccgtcgcta cgttcctgct cgagacgccg acactcacgc tcggctgcct
     2341 cacgcgcttc ctgctcggcg cgctggcggg cacgggcttc tgcctccgcc ctctcctgac
     2401 gctcgcgctc tgcctgggcc aggagcgcgg cctcggcctc cagtcggcgg cgctcggcgg
     2461 cgagccgctc ctcctcggca gcccgcagtt tccgcgcggc ctcttcctcg gcagcccgct
     2521 gcttccgggc ggcctcttcc agggcaagct gctcggcgcg ctcacgctct aggcgctcgc
     2581 gcgcagcgcg ctcctcggct tccctccggg ccttggcctg ccgctcttct tccgcgatct
     2641 tggcgcggcg ctcggcctca gtgacctgac ggcacatcag gttcccgccc ccggtatcga
     2701 agagtgtgct gcagtcctgc tcgctcacct tgcgcgtcgg atccatcggc ggcgggtagg
     2761 ctccatcctg cagtgtggta ccgcagccgg ccgcgagcac gaggaggagg acgacgattt
     2821 tgttcttgtt catggctctc ccttcaggtt cgttaggcgc gcagcgccag aactgcctga
     2881 tttgacttcg aagcgacgat acgcgcagcg cgctcggcgc gctgcgacaa aatccccaca
     2941 tcagcagcgg cggtattcgc ccttcccggt gcaagccgcg agcatcgcgg gcgcgatcat
     3001 gttcgtcaaa tccaggggga gacggcgccg gggaggggat gcccctggcg atctgggggg
     3061 ccgtggggac gcggcccgct ttgacgcggt agttgggggg aaggaaaggc cggcgctcgc
     3121 cggccttttc ttgtttcata ccgtgggata gggcttgcgc gcgacctccg ccgtactaca
     3181 ttgagggcag acgccgtaac gccgggagga ggtaagccat gagttctcgt accccgcagg
     3241 ttctgctcgt cctcgctctg gccgccgtcc tgccgcccgc atgggcgcac ggcgacgagg
     3301 cgcgccagtg ccgcgcgcgc aacgactcga ttcccacctc tctaggtgcc gacggctggg
     3361 ctgcggtcga tggacccgtc accggcggct gcgctgcagg ccgcacgcgc gtgttccacg
     3421 tttacgaccg ccggcagctc gtgctcgcgc tgaacaacga cctcttattg ctgctcgagc
     3481 atctcttccc ccggcttgac gtaaaacggc gccacctgct cgaccaccgc tcgaagatca
     3541 tctacatcca ccgcaccatc gacctgaacg tcgaccaccg caaccggccg ctggtggaag
     3601 aggactacat gcggatgtgc ggctatacgg cgcacgacac ctactacgac ccggcgaccg
     3661 gcaaccagga cggcagcggc ggcttcttcg gcgcctacaa ggccgagtac gacccgaacc
     3721 ggtggatccg ccagtcgctc gacacggcgg acaaccgccc gcccgcgctg agcggcccgc
     3781 tcgaggaggc gcgcctgtgc ttccagcgcg cgcaggccga gcgcgtgatg atcgacgtcg
     3841 gctcgaacac ctcgatcatc ggcgtgggac cggatgcgaa gatcatcaac ggcggcctgc
     3901 gcatcggctt catgaaccag ttcgacccca acgatcgcga caactaccgc gcggagaacg
     3961 tggtcgtgcg caacatcact ttcgccgacg cgttcgacat gttcccgggc tgggatccga
     4021 aggactcgtt cagcatcacc atcaccaaca cggacggctg ccaggcggcg ttcgacgagg
     4081 cgaccgactc cgggccgcac cgctgcccga cccgccgcgg cgggcgctgg aacaccgagt
     4141 acgacggcat ctcggtgatg aacgccaagc acgtctggat cgaccacaac accttcaccg
     4201 acgagccgcg cttcgacacc cagttcccgc cggtgttcgg cgcgcccttc aacgagccga
     4261 cgcagaagac cgcgcaccac gacggccacg tcgacgtgac gctgcttgcc accaaggtga
     4321 cgatctcgaa caaccacttc ctgcgccacg acaagggcaa cctgctcggc ggctcggacg
     4381 tggccaacct ggtgccgggc tacgggccgg gcaagatcga cgtcaccttc cacggcaact
     4441 actaccagaa catgatccag cgcatgccgc gcgtgcgctt cggccgggtg cacgtctaca
     4501 acaacgtctt cgacgccaac cggctggcga acgccgagta ccgcctgggc gacacctggg
     4561 cgaccggcac cgcggcgaaa ctcgtcaccg agaacaacct gttcgacatc ctcaacaaca
     4621 acctcaccat cccgcgcatc atcaactacg cctcgaccgt cgcgaacttc gacctctgcg
     4681 tcagcgcggg cttcaccgct gcggagtgcg gcacgtacta ctacgacacg ggcacctgga
     4741 tcacgatgaa ccgcgtgacc ggcaccccgc caaccgccac gacgctcttc gacgcctttg
     4801 ccgcggtgaa agccatccag gaaagcagcg ccgccaacgc gcccctcttc aagctcgacc
     4861 cggcggatcc ggcggtgttc tggtatccca gccagaccta cagctatctg ctcgcgccgg
     4921 tcagcacgga aacggagcgc accgagctgc gcaccacggt catcaacaac gcgggcgctg
     4981 gcaagctcta gcggactgtt tcaacttcga ggaaaggccg gctcgcgccg gcctttttct
     5041 ttcggctgat ggaacttgat ggagcgccgg catgccatcg caattttcga tagtaggacg
     5101 ctaagtgctt gcagccgcat gacctttgtg acgggagggt aaatcccaga gggcgggatg
     5161 ccgcaaagca acaaagtcat ccttccgaaa attgtttgag gtaatcttct tactgcaagt
     5221 cgtccgacca aagaaacaga tacctcggga ggcgggagat ggacgtcaag gatctccgat
     5281 atttcgtcgc ggtgtacgag tccgagggct tctcgcgagc ctcgcgcgag ctcggcactg
     5341 tgcagtcgaa cgtttcggcg cgcatccgca atctcgaggg gacgctgggc atcgcgctgt
     5401 tcgagcgccg ctataggcgc gtggcgccga cggcgggcgc ggacaagctc tatcgccacg
     5461 cgaagaaggt gatcagcacg ctcgccgacg cggagcgcgc gctgcgccgc tcgcgcgtca
     5521 agggctttac gcttctcgag ctgctggtgg tggtggtgat catcgggctg ctagctggtt
     5581 ttgtcgcgcc acgctatttc agccaggtcg ggaaatccga ggtgaacgtg gccaaggcgc
     5641 agatcgacgc gctggagaag gcgctcgacc agtaccgcct cgacgtcggg cgctatccgt
     5701 cgaacgagct cggcctgaag gcgctggtcg acaagccgac cagcgagccg aagtggaacg
     5761 ggccctacct caagaagcag gtgccgctcg atccgtgggg caaggcctat atctacaaga
     5821 tgccggggga aaagggagat ttcgacctgc tgtcctacgg ccgcgacggg gcggcgggcg
     5881 gctccggcga agacgcggac atcaccaacc gctagggcgc catgcgcttc cacctgcgcg
     5941 cgatcacgaa cgagggacgc atcgagtcgc tggactacca ggcggtcgat gagcagtccg
     6001 cgcgccagca ggccgagagc cgcggctaca cggtgctgca ggtgcagagc aggcgcagcg
     6061 tttcgctgct gcgccagtcg cgctttccgg tgcagctctt cagccaggag ttgctggtgc
     6121 tgctgagcgc gggcctgccg ctggtggacg cgatcgagac cctgggcgag aaggaaaaaa
     6181 gggacgagtg gcgcgctctc ctcggcagcc tggtggcggt gctgcgtcag gggcagccgt
     6241 tctcggtcgc gctcgagcag cagccggcgg tgttctcgca gctctacgtc gccacggtgc
     6301 gcgcggccga gaagacgagc gacctcgcgc cggcgctctc gcgctacgtc gcctaccagg
     6361 cgcagatcga ggcgatccgc aagcgcgcgc tcaacgcgtc gatctatccg atggtcctga
     6421 tcggcgcggg cgccgtggtg agcctgtttc tcctgttcta cgtcgtgccg cgcttcggca
     6481 agatctacga ggaccgcacc accgagctgc cgttcttctc gaagatcctc atcggctggg
     6541 ggcaggtcgt cgaggggcac gcaatgatcg cgctcggctt gctggcgggg ttggtgattg
     6601 cgatcatctt tgccgcgcgc gacccccgcg ccaaagcgtt tctgggagac atcctttggc
     6661 gcgtgccggc gatcggcgag cgcctcaagg tgtaccagct cgcgcggctc taccgcacga
     6721 ccgggatgct gctgcgcggc gggatgccgc tcgtctccgc gctcggcatg agcgccgagc
     6781 tgctgcaccc tgtgctgcgc gagcgccttg cggccacgag caaagcgatc agcgaaggcc
     6841 tcggggtgtc ggaatcgttc gacaagaacg ggctcgccac gccggtcgcg atgcgcatgc
     6901 tggtggtggg cgagcagggc ggcaacatgg gcgagatgct ggatcgcgcc gccggcttcc
     6961 acgacgagga gctcgcgcgc tgggtggact ggttcacgcg catgttcgag ccgctgctga
     7021 tggcgctgat cggcctggtg atcggcgcga tcgtgatcct catgtacatg cccatcttcg
     7081 agctggcggg gaacctgcga tgatcgaagc gaaaacaatc gccgaagcgc gcgccgaggc
     7141 gcacgccgcc accggcagcc gccgcgtggt cgacgtgctg gaagagaagc ttgcgctcca
     7201 gcccgacgaa ttcgccgcca agctggcggc gaccttccgc cttgccgtgc tgcgcatgga
     7261 cgagctgcgc cgcctcgagc ccgcgttcga cctcgtgccg ctcagcgagt gcgggcgcaa
     7321 ggcgtgcgtc ctgttgcgcg ccgaaggcgg agagctcctg atggcgaccg acgacccgtt
     7381 ctccgccgag caggtggcgt gggcggccga gcgcatccgc gcgccgttcg cctggcgact
     7441 ggtgcaccgc ggcgacctcg tcgcctatct cgcgagccac gaggagacgc tgcgcgcgct
     7501 cgacgcggtg cgcgccgacg tcggcgtcgc gggcgacgag aacgtgggcg cggaagacct
     7561 ttcgctcaag gcgatcggcg aggagaagag cgaggtggtg cgcctggtgc gctccactct
     7621 gcgcgacgcg ctcaagatcg gcgcgagcga catccacctc gagaccgtcg ccgcgggcct
     7681 ggtgatcaag ttccgcatcg acggcatcct gatccagatc aaagtgatcg aggaccgcgc
     7741 gcaggccgag caggcgatcg cgcgcatcaa ggtgctcgcc gagctcgacg tcaccgagcg
     7801 ccgcgtgccg caggacgggc gcttcaaggc gatcgaccgc ctgcgcgcga tcgatttccg
     7861 cgtctcgatc atgccgagca tccacggcga ggacgcggtg ctgcgcgtgc tcgacaagca
     7921 gtcgctctac gagagcacgc gccagcagct ttcgctcgac agcctgggct tcgaggaggc
     7981 cgacatcaag gcgctgcggc gcctgtcgca cgagccgcac ggcatgctgc tcgtcaccgg
     8041 cccgaccggc agcggcaaga ccaccacgct ctacgccgcg atcaccgaga tcaacaacgg
     8101 ccaggacaag atcgtcacca tcgaggaccc ggtcgagtac cagctccccg gcgtgctgca
     8161 gatcccggtg aacgagaaga agggcctcac cttcgcgcgc ggcctgcgct cgatcctgcg
     8221 ccacgacccc gaccgcatca tggtgggcga gatccgcgat cccgagaccg ccaacatcgc
     8281 ggtgcagtcc gcgctcaccg gccacctcgt cttcaccacg gtgcacgcca acaacgtgtt
     8341 cgacgtgatc ggccgcttca tgcacatgaa cgtcgacctg tacggcttcg tctcggcgtg
     8401 caacgccatc ctcgcgcagc gcctggtgcg catggtctgc ccgcactgcg cggaggcatt
     8461 cgctccggac aaggcgctgc tggaagagtc gcgcctgacc gaccagcagg tgcagggctg
     8521 gcagttcaag gtcggcaaag gctgccgcga atgccgcggt gccggctacc gcgggcggcg
     8581 tgcgatcgcc gagctcctca ttctcaacga cgagctgcgc gagatgatca tcggacgctc
     8641 gccgatccgc gccctgaagg aggcggcgac gaggaacggc acgcgcttcc tgcgcgaggc
     8701 ggcgct
//
LOCUS       DATBSZ010000208         6493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_378316, whole genome shotgun sequence.
ACCESSION   DATBSZ010000208 DATBSZ010000000
VERSION     DATBSZ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378316"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /gene="rmuC"
                     /locus_tag="VIG70_13355"
     CDS             complement(<1..1116)
                     /gene="rmuC"
                     /locus_tag="VIG70_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY8251586.1"
                     /translation="MVEIFLALTLFAAISTAGLLGVSLLRARSAEREARDELSRSLAT
                     FSQTLSTQMGAAASVQNGQMDGLRKAIDDNLSRIQDQQRLAARETRDTLEQKLDRLQQ
                     DNSAKLEQMRQTVDEKLHATLEKRLSESFRQVSERLELVHKGLGEMQTLAAGVGDLKR
                     VLSNVKTRGVLGETQLAALLEQVMAPEQYEKNVATRPGSNARVEFAIKLPGRDDGGPV
                     LLPIDAKFPLEDYQRLQAAQEAGDKSALEMAEKALEARVKLEAKTIAEKYVEPPHTTD
                     FALLYLPFEGLFGEVLRRPGLFDQIQRQFRVTICGPTNLLAYLNSLQMGFRTLAIQQR
                     SSEVWKVLGTVKSEFGKFAEVLANTKRQLQTVANTIDQ"
     gene            complement(1134..3614)
                     /locus_tag="VIG70_13360"
     CDS             complement(1134..3614)
                     /locus_tag="VIG70_13360"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8251587.1"
                     /translation="MRQSWKMLLRDWRAGELRVLALALTVAVAAITSVAFFADRVGQG
                     LARDAHQLLGADLVMVADQPWPREVAAQLERAGVQRAEAINFISMASGKESSLSGVKG
                     VSENYPLRGRLRIASAPGAPDAPAERGPAPGTVWLEERLVTALGAPVGSRIKLGRAEF
                     EVAAVITLEPERSASFFNIAPRLLMNLADVPSTALVQTGSRVWYYLYAAGPAERIRSL
                     EESVRPKLGRGQRVENLETGRPEIRSAVDRAQRFLGLTALLAAILAGVAVALGARRFV
                     ERHLDGCAVMRCFGATQSQLLRLHAAEFLILGLAACAAGCAVGFVAQEAIAATVAEVV
                     QAELPPPRALPAVQGFLVGLVLLLGFALPPLVQLKNVPAVRVMRRESGAPKGGTIVAY
                     AAGLISLSALLVWQAGDLPLGLTVVGGFAAAVAVFFAAAWGLLRLLTHRNIVGLLRNS
                     MLRYGLANLRRHARGNAVQIASLALGLTAVLLLTFTRNELVEAWRRSAPPDAPNRFLI
                     GVQPEQLEPLQAFFAAHKVAVPELYPMVRGRLVAVNGAPVSEADYSEERAKRLVEREF
                     NLSYMTELPGHNQVTAGAWFSPAERALSVEQGIASRLGWKLGDELTFAVGSERFSARI
                     TSLRKLRWDSMKVNFFVVTAPAVLEGFPVSFISAFRLEPEAQPVITELAERFPNITVI
                     DAAAAVRQAQGVIDQLISAVQFVFLFALAAGLLVLYSALVATEDERRREAAVMRVYGA
                     SRAQVTGTQRAEFLAMGAAAGLLATIGAAVIGQVLARRVFELDLPPSAALWIAGPLAG
                     LALLSLNAWLSSRKVLRASPALTLRDSV"
     gene            complement(3611..4288)
                     /locus_tag="VIG70_13365"
     CDS             complement(3611..4288)
                     /locus_tag="VIG70_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251588.1"
                     /translation="MKNNNSVLSARGIGKTVKSGANDLVILREIDLAVTRGEAVAVVG
                     ASGSGKSTLLAILAGLDTPSRGNVELDGQDLFGMDEDQRARLRAMSVGFVFQSFQLLP
                     ALTALENVMLPLELANDDEADAKAKEVLERVGLGERLHHYPKHLSGGEQQRVALARAF
                     VVRPKLLLADEPTGSLDADSGAEVIRLLFEMNREYGTTLVMVTHDESLAARCGRIVRL
                     AAGRIVA"
     gene            4278..4868
                     /locus_tag="VIG70_13370"
     CDS             4278..4868
                     /locus_tag="VIG70_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY8251589.1"
                     /translation="MFFIVLWLPGTAAAEKSILVMGDSLSSAYGIAQSRGWVALLGER
                     LKREHSDYIVVNASISGDTSGGGRARLKPLLEKHRPAIVILALGGNDGLRGMPITQMR
                     TNLAASIREAQASGARVLLVGVKMPPNYGEVYTQGFGAAFVELAKTHRIPLVPFVLED
                     FAGKPEFFLPDRIHPNEQAQPLMLERVLPVLRPLLN"
     gene            complement(4861..5904)
                     /gene="selD"
                     /locus_tag="VIG70_13375"
     CDS             complement(4861..5904)
                     /gene="selD"
                     /locus_tag="VIG70_13375"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEY8251590.1"
                     /translation="MNAPERIRLTEFSHGGGCGCKIAPAVLAEMLASAPIRNLPAELL
                     VGTETSDDAAVYRLNDTQALVATTDFFSPVVDDPYDFGRIAATNAISDVYAMGARPIM
                     ALALVGMPLDKLPVSVIRKVLEGGESVCAQAGIPVAGGHSIDTLEPIYGLVALGLVHP
                     DRVKRNSTAKAGDVLILGKPLGIGILSAALKKGKLSEPGYRQMIEWTTKLNTPGEALA
                     SMPAVHALTDVTGFGLAGHLLEICRGSHLSAEVDFSSIPVIAEALDWVKQGVATGAST
                     RNWSGYGHDVEFGGEEWKQKVLTDPQTSGGLLVACAPEARDSVLEIFRNKGFGEARVI
                     GRLSAGPARIRVS"
     gene            5923..>6493
                     /gene="mnmH"
                     /locus_tag="VIG70_13380"
     CDS             5923..>6493
                     /gene="mnmH"
                     /locus_tag="VIG70_13380"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEY8251591.1"
                     /translation="MDLKKFDAIIDVRSPAEYAEDHIPGAISAPVLDDAERAQVGTMY
                     KQASPFDAKKIGAALIAKNISQHVDELFKDKPKSWHPLVYCWRGGKRSGAMAHILREI
                     GWNAATLEGGYKAYRRWVVQALEQIPEKLDFRVIHGPTGSGKSRLLGALGRASGPVLD
                     LEELAAHRGSVLGNLPDRPQPSQKMFESAL"
BASE COUNT         1042 a         2233 c         2226 g          992 t
ORIGIN      
        1 ctggtcgatg gtgttggcca ccgtctgcag ctggcgcttg gtgttcgcca gcacctcggc
       61 aaatttgccg aactcggatt tcaccgtgcc gagcaccttc cagacctcgg aggagcgctg
      121 ctgaatggcg agcgtgcgga atcccatctg caggctgttg agataagcga gcaggttggt
      181 cggcccgcag atcgtcacgc ggaactggcg ctggatctgg tcgaacaggc ccggcctgcg
      241 cagcacctcg ccgaacaggc cctcgaacgg caggtagagc aacgcgaagt cggtggtatg
      301 cgggggctcg acgtactttt cagcgatggt cttggcttca agcttgacgc gcgcctcgag
      361 cgccttctcg gccatctcga gcgccgactt gtcgccggct tcctgcgccg cctgcagccg
      421 ctggtagtcc tccagcggga acttggcgtc gatcggcagc aggaccggcc cgccgtcgtc
      481 ccggccgggc agcttgatcg caaactccac ccgggcgttc gagccgggac gcgtcgccac
      541 gttcttctcg tactgctccg gcgccatcac ctgctcgagg agggcggcga gctgcgtctc
      601 gccgagcacg ccgcgggtct tcacgttgga cagcactcgc ttgaggtcgc cgaccccggc
      661 cgccagcgtc tgcatctccc ccagcccctt gtgcaccagc tcgagccgct ccgacacctg
      721 gcggaacgac tcggacaggc gcttctcgag agtcgcatgc agcttctcgt ccaccgtctg
      781 gcgcatctgc tcgagcttgg cggagttgtc ctgctgcagc cggtcgagct tttgctccag
      841 ggtgtcgcgc gtctcgcgcg cggcgagccg ctgctggtcc tggatgcgcg aaaggttgtc
      901 gtcgatggcc ttgcgcaggc cgtccatctg gccgttctgc acgctcgcgg ccgcgcccat
      961 ctgggtgctt agcgtctggc tgaacgtggc gaggctgcgc gagagctcgt cgcgggcttc
     1021 gcgctcggcg ctcctcgccc gcagcaggga gacgccgagc aggccggcag ttgagatcgc
     1081 cgcgaacagg gtcagcgcga ggaagatctc gaccatgctg tatattaata cagctacacg
     1141 gaatccctca acgtgagcgc cggggaggcg cgcagcactt tgcgcgacga gagccatgcg
     1201 ttgagcgaga ggagcgcgag gccggcgagc gggccggcga tccacagcgc cgccgaaggc
     1261 ggcaggtcga gctcgaacac gcggcgcgcg agcacctgcc cgatcacggc cgcgccgatg
     1321 gtggcgagca ggcccgcggc cgcgcccatc gcgaggaact cggcgcgctg cgtgccggta
     1381 acctgcgcgc gcgaggcgcc gtacacccgc atcaccgcgg cttcgcgccg gcgctcgtcc
     1441 tcggtggcga cgagcgcgga gtacagcacg agcagccccg ccgcgagcgc gaacaggaac
     1501 acgaactgca ccgcgctgat gagctggtcg atcacgccct gcgcctggcg caccgcggcc
     1561 gcggcgtcga tcaccgtgat gttcggaaag cgctcggcga gctcggtgat caccggctgc
     1621 gcttcgggct cgaggcgaaa cgcgctgatg aagctcaccg gaaagccttc gagcaccgca
     1681 ggcgcggtga cgacgaagaa gttcactttc atcgagtccc agcgcagctt gcgcagcgag
     1741 gtgatgcgcg cgctgaagcg ctccgagccc acggcgaagg tgagctcgtc gccgagcttc
     1801 cagccgaggc ggctggcgat cccctgctcc acggagagcg ctctttccgc tggagaaaac
     1861 caggcgccgg cggtgacctg gttgtgcccc gggagctcgg tcatataaga aaggttgaac
     1921 tcccgctcga ccaggcgttt cgcgcgctct tcgctgtaat cggcctcgct caccggcgcg
     1981 ccgttcacgg cgaccaggcg cccgcgcacc atcggataga gctcgggcac cgcgaccttg
     2041 tgcgcggcga agaaggcctg cagcggctcg agctgttcgg gctgcacgcc gatcaggaag
     2101 cggttgggcg catccggcgg ggcgctgcgc cgccaggcct cgaccagctc gttgcgcgtg
     2161 aaggtgagga gcagcaccgc ggtcagcccg agggcgaggc tcgcgatctg cacggcgttg
     2221 ccgcgcgcgt ggcgcctgag gttggcgagg ccgtagcgca gcatcgaatt tcgcagtaac
     2281 cccacaatgt tccgatgggt gagcagacgc agcagacccc aggccgcggc gaagaacacc
     2341 gccaccgcgg cggcgaagcc gccgaccacg gtgaggccga gcgggaggtc gcccgcctgc
     2401 cacaccagga gcgcggagag ggagatcagc cccgcggcgt acgcgacgat cgtgccgcct
     2461 ttcggcgcgc cggattcgcg ccgcatcacg cgcaccgcgg gcacgttctt cagttgcacc
     2521 agcggtggca gcgcgaagcc gaggagcagc acgagcccca ccaggaagcc ctgcaccgcg
     2581 ggcagcgcgc gcggcggcgg cagctcggct tggaccacct cggccacggt tgcggcgatc
     2641 gcttcctgcg cgacgaagcc gaccgcgcag cctgcggcgc acgccgccag gccgaggatc
     2701 aggaactcgg ccgcatggag ccgcaggagc tgcgactgcg tcgcgccgaa gcagcgcatg
     2761 accgcgcagc cgtcgagatg gcgctcgacg aagcgccgag cgccgagcgc gacggcgacg
     2821 ccggcgagga tcgcggcgag gagcgccgtg aggccgagga agcgctgcgc gcggtcgacc
     2881 gccgagcgga tctccgggcg cccggtttcg agattttcca cccgctgccc ccggccgagc
     2941 ttcggccgga ccgattcctc cagcgatcgg atgcgctcgg caggcccggc cgcatagagg
     3001 tagtaccaga cccggctgcc ggtctggacg agcgcggtcg acggcacgtc ggcgaggttc
     3061 atcaggagtc gcggcgcgat gttgaagaag ctcgcgctgc gctcgggctc gagggtgatc
     3121 accgcggcca cctcgaactc ggcgcggccg agcttgatgc gcgagcctac cggcgcgccg
     3181 agcgcggtga ccaggcgctc ctcgagccac accgttccgg gagcggggcc gcgctcggcc
     3241 ggcgcgtcgg gcgcaccggg cgcactggcg atgcgcaggc gcccgcgcaa ggggtagttc
     3301 tccgaaaccc ccttcacgcc ggagaggctg ctttcctttc ccgaagccat gctgatgaag
     3361 ttgatcgcct cggcgcgctg cacgccggcg cgctcgagct gcgccgcgac ttcgcgcggc
     3421 cacggctggt cggcgaccat cacgagatcc gcgccgagga gctgatgcgc gtcgcgcgcc
     3481 agtccctggc cgacgcggtc ggcgaagaag gccacgctgg tgatcgccgc gacggccacg
     3541 gtcaatgcaa gcgcaagcac tcgcagctcg cctgcgcgcc agtcgcgcag cagcatcttc
     3601 caggactgcc tcatgcgacg atccgcccgg cggcgagccg cacgatgcgg ccgcagcgcg
     3661 cggcgaggct ttcgtcgtgc gtcaccatga cgagcgtggt gccgtactcg cggttcatct
     3721 cgaacagcag ccggatcacc tcggcgcccg agtccgcgtc gaggctgccg gtgggctcat
     3781 cggcaagcag gagcttcggc cgcaccacga acgcgcgcgc cagcgcgacg cgctgctgct
     3841 cgccgccgga aaggtgcttc gggtagtggt gcagccgctc gccgaggccc acgcgctcga
     3901 gaacctcctt tgcctttgca tctgcttcgt cgtcgttcgc gagctcgagc ggcagcatca
     3961 cattctcgag cgcggtcagc gccggcagga gctggaacga ctggaacacg aagcccacgc
     4021 tcatcgctcg cagccgcgcc cgctggtctt catccatgcc gaaaagatcc tggccgtcga
     4081 gctccacgtt cccgcggctg ggcgtgtcga gcccggcgag gatcgcgagc agggtcgact
     4141 tgccggagcc ggaagcgccg accacggcga ccgcttcgcc gcgcgtgacc gcgaggtcga
     4201 tctcccgcag aataacgagg tcgttcgcac cgctcttcac ggtcttcccg atgccgcgcg
     4261 cgctcagcac gctattgttg ttcttcatcg tcctgtggtt gccgggaacg gcggcggcgg
     4321 agaagtccat tctggtgatg ggggacagcc tgtcctcggc ctacggcatt gcgcagagcc
     4381 gcggctgggt ggcgctgctc ggcgagcgcc tcaagcgcga gcactcggat tatatcgtcg
     4441 tcaacgcgag catctcgggg gacacgagcg gcggcggccg cgcgcgcctc aagccgctgc
     4501 tcgagaagca ccggccggcg atcgtgatcc tcgcgctcgg cggcaacgac gggctgcgcg
     4561 gcatgccgat cacgcagatg cgcaccaacc tcgctgcgtc gatccgcgag gcgcaggcaa
     4621 gcggcgcgcg cgtgctcctg gtcggcgtga agatgccgcc caactacggc gaggtgtata
     4681 cgcagggctt cggtgcggcc ttcgtcgagc tggccaagac ccaccgcatt cctttggtgc
     4741 cgttcgtgct cgaggatttc gccggcaagc ccgagttctt cctgcccgac cgcattcatc
     4801 ccaacgagca ggcgcagccg ctgatgctgg agcgcgtctt gccggtcctg cgtccgctgc
     4861 ttaactgacc ctgatcctgg ctggacccgc gctcaggcgc ccgatgaccc gcgcctcgcc
     4921 gaagcctttg tttcgaaaaa tctccagcac ggaatcgcgt gcttccggag cgcaagccac
     4981 gagcaatcca ccgctggttt gcgggtcggt cagcaccttt tgcttccact cctcgccgcc
     5041 gaattccacg tcgtggccgt atccgctcca gttccgggtc gacgcgccgg tggcgacgcc
     5101 ctgcttcacc cagtcgagag cctcggcgat gacgggaatg gaagaaaaat ccacttcggc
     5161 ggaaaggtgc gatccgcggc agatctcgag caggtggccg gcaaggccga agccggtcac
     5221 gtcggtcagc gcgtgcaccg cgggcatcga ggcgagcgcc tcgcccggcg tattgagctt
     5281 ggtggtccat tcgatcatct gccggtagcc cggctccgac agcttgcctt tcttcagcgc
     5341 ggcggaaagg atgccgatgc cgaggggttt tccgagaatc agcacgtcgc cggctttggc
     5401 ggtgctgttg cgcttcacgc gatccgggtg gaccaaaccc agcgccacca ggccgtagat
     5461 cggctcgagc gtgtcgatcg aatgcccgcc ggcgaccggg atgccggcct gcgcgcaaac
     5521 cgactcgccg ccttcgagca ccttgcggat cacgctgacg ggcagcttgt cgagcggcat
     5581 gccgaccagc gcgagcgcca tgatcgggcg cgcgcccatc gcgtacacgt ccgagatcgc
     5641 gttggtcgcg gcgatgcggc cgaaatcgta agggtcgtcg accaccgggc tgaaaaaatc
     5701 cgtggtggcg acgagcgcct gcgtgtcgtt cagccgatac accgcggcgt cgtcggacgt
     5761 ctccgtgccg acgagcagct ctgccgggag atttcggatg ggagcggaag ccagcatttc
     5821 cgccagcacg gcgggcgcga tcttgcagcc gcagccgccg ccgtggctga actcggtcag
     5881 gcgaatgcgt tcgggagcgt tcataagcgt agaattttac agatggattt gaagaagttc
     5941 gacgcgatta tcgacgtgcg ttcacccgca gagtacgccg aagaccacat ccccggcgcg
     6001 atctcggcgc cggtgctcga cgacgccgag cgcgcgcagg tcggcacgat gtacaagcag
     6061 gcctcgcctt tcgacgcaaa aaaaatcggc gccgccctga tcgcaaaaaa catcagccag
     6121 cacgtggacg agcttttcaa ggacaagccc aagagctggc atcccctagt ctactgctgg
     6181 cgcggcggca agcgctcggg cgcgatggcc cacatcctgc gcgaaatcgg ctggaacgcc
     6241 gccacgctcg aaggcggcta caaggcctat cgccgctggg tcgtgcaggc gctggaacag
     6301 atcccggaga agctcgactt tcgcgtgatt cacggcccga ccggcagcgg caagagccgc
     6361 ctcctcggag cgctcgggcg cgccagtggg ccggtgctcg acctcgagga gctcgccgcg
     6421 caccgcggct cggtgctcgg caacctgccg gatcgcccgc agccctcgca gaaaatgttc
     6481 gaaagcgcgc tgc
//
LOCUS       DATBSZ010000209         5736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1471896, whole genome shotgun sequence.
ACCESSION   DATBSZ010000209 DATBSZ010000000
VERSION     DATBSZ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471896"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VIG70_13385"
     CDS             <1..674
                     /locus_tag="VIG70_13385"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY8251592.1"
                     /translation="PAINPNLPYDPKSDFVDVTPLASQPMVLVVGPSSGVASVADLIA
                     RARAQPGGVNYGSAGIGSGAHFNSEKFRIAAGIDVVHVPYKGGAEAIHDTIAGRLTFT
                     FNTITLALPHIRERRVVVLGVSSAQRSSLLPGTPTIAEAGMPGFEYTFWNGLWAPAGT
                     PTAVVERIARDLGRVVALPEVRERLAALGAEPMSMAPAEFARFVRSEIEDAARIARAA
                     GIKLQ"
     gene            complement(661..1584)
                     /locus_tag="VIG70_13390"
     CDS             complement(661..1584)
                     /locus_tag="VIG70_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY8251593.1"
                     /translation="MRVTLIHNPGAGKQDASSAKALLKMLRREGHKPRYQSAKERGWA
                     RVLDKKAGLVVVAGGDGTVANVTRHMVGRGVPVAVLPSGTANNIARSLGLLKRPFEEL
                     VRGWRDAKRVRLDVGIACGPWGERYFVEGLGAGLFAGLLVRSEENQAKREKEKKSKRP
                     KHVVDNALRRLKEAAERDEPVEIAAQLDGVDISGRYLLFEAVNLPFIGPNLYIAPDNK
                     AGDGQLEVVLVSESQRGRLVRYLDHWQESRERLSLLPSRRGKHLQIEWTGFPLHIDDK
                     LRPKQKAEADDVAGLVDVRVNGAAVEFLTVA"
     gene            1608..2360
                     /gene="surE"
                     /locus_tag="VIG70_13395"
     CDS             1608..2360
                     /gene="surE"
                     /locus_tag="VIG70_13395"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY8251594.1"
                     /translation="MRILITNDDGVYSPGIAALAQVASRFGEVRIVAPDVEMSSASHS
                     ITASRPVTYKRTPLPGLDAYRVNGTPGDCVMLGTTLWEKVDLVLSGINIGTNLGNAVW
                     HSGTLAGAKQAALLGLRGIAFSAPATDREQPSFEALKPWVATVLEALLEAPDMPLVNV
                     NFPAEAPRGTHWTRQSMRHYDGKVVPAKDPMGRTHYWFTVVPVEATEEGTDRWAIEQR
                     YVSITPLRLDLTDQEKLAEARKVESWKKLKFA"
     gene            complement(2362..3507)
                     /locus_tag="VIG70_13400"
     CDS             complement(2362..3507)
                     /locus_tag="VIG70_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEY8251595.1"
                     /translation="MSSSRSEAFFASCPRGLEELLLQELSSFGAEAPAQAPGGVAFSG
                     SWEIGYRANLWSRIASRILWRVGEFPYRNEKDLYDAARAIAWPKYFSVQKKIRVNITA
                     QKSPLKSLEFATLKVKDAVCDLFRDKVGSRPDVDRAAPDVRIHAFLEEAKGTLYLDTS
                     GEALFKRGWRMDIAEAPIRENLAAGILMLSGWKFDQPLLDPMCGGGTLLSEAAAMARG
                     RAPGAKRAFGFERLSVFDSGLWERIKNERTVQPVEPRLYGSDTDPEALQAARRNLGAA
                     GVERWVKLEQADVLERAAPEAAGVMVANPPYGERIGSADELAAFYPKLGDALKKKFAG
                     WSCFFFTADLRMAKLIRLQPARRTPLWNGSLECRLYEFRIVSGSNRG"
     gene            complement(3488..3904)
                     /locus_tag="VIG70_13405"
     CDS             complement(3488..3904)
                     /locus_tag="VIG70_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY8251596.1"
                     /translation="MLWVKSLHIIFMVTWFAGLFYLPRLFVYHAQADDRISLERFKVM
                     ERKLFWGIMTPGGVLTIVFGVWLWLGWFTPATGWLHAKIALVALLAGYHLWCWRLMRD
                     FAAERNRRGHVWFRWFNEFPTLVLFATVLLVVFKPF"
     gene            complement(3907..4656)
                     /locus_tag="VIG70_13410"
     CDS             complement(3907..4656)
                     /locus_tag="VIG70_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8251597.1"
                     /translation="MRWISVWRRNMLVWRKLFAAAVLSNLADPLIMLFGLGYGLGALL
                     PSVEGMSYLAFFAAGQLCTATMFTASFESMFSGFSRMHGQKTWDAILYAPLTIDDIVA
                     GEIVWAASKAWLTGSTILGVVVVFGLASSPWVLLALPAAFLVGLAFSAVGLIMCVLAR
                     GWDFFSYYMTLMMTPMMMISGVFFPAEQLPAPLLAVAKALPLYHGVQIVRPLVAGGPP
                     PDLWLHAAVLLAYAAAGYTLAIYFARRRFSS"
     gene            complement(4657..5559)
                     /locus_tag="VIG70_13415"
     CDS             complement(4657..5559)
                     /locus_tag="VIG70_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8251598.1"
                     /translation="MTALSARALRKSYGDLEVVSGVDLAVAPGECFGLLGPNGAGKTT
                     TLKLCLGLIEPDGGEIELLGEPVPRRAREARRNVGVVPQFDNLDPDFTVEENLVVYAR
                     YFGIAKEEIRERISALLEFAGLAGRGDSKITTLSGGMKRRLTLARALVNDPQLVFMDE
                     PTTGLDPQARHLIWERLRRLTQEGKTLVLTTHFMEEAERLCHRLAIMDRGRIIAEGSP
                     RALIAEHIEPQVLEVHGPGYETWMQRAKGLAPRVERVGDTVYCYGEDIEPVLKSISGE
                     LSYLHRPASLEDVFLKLTGRDLRD"
     gene            complement(5556..>5736)
                     /locus_tag="VIG70_13420"
     CDS             complement(5556..>5736)
                     /locus_tag="VIG70_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8251599.1"
                     /translation="PVPEGTLVIHGENDDTVPLAEVLEWARPQELPVVLVPGADHFFH
                     RKLHVLKRIVQARWK"
BASE COUNT          969 a         1986 c         1888 g          893 t
ORIGIN      
        1 acccggcgat caatccgaac ctgccttacg accccaagag cgatttcgtg gatgtgacgc
       61 cgctcgcgag ccagccgatg gtgctggtgg tcgggccgtc atcgggcgtc gcgagcgtgg
      121 cggacctgat cgcccgggcc agggcgcagc cgggcggggt gaactacggc tccgcgggca
      181 tcggcagcgg cgcgcatttc aattccgaga agttcaggat cgcggcgggt atcgacgtgg
      241 tccacgtccc gtacaagggc ggggccgagg cgatccacga cacgatcgcc gggcgcctca
      301 cctttacctt caacaccatc acgctcgccc tgccgcacat ccgcgagcgc cgcgtggtgg
      361 tgctcggggt gagcagcgcc cagcgctcga gcctgctgcc gggaacaccg accatcgccg
      421 aggccggcat gccgggcttc gagtacacgt tctggaacgg gctgtgggcg ccggcgggca
      481 cgccgacggc ggtggtcgag cgcatcgcac gcgatctcgg ccgcgtcgtt gcgctgcctg
      541 aggtgcgcga gcggctcgcc gcgcttggcg ccgagccgat gagcatggcg cctgcggaat
      601 tcgcgcgctt cgtgaggagc gagatcgagg acgcggcgcg catcgcgcgc gccgccggca
      661 tcaagctaca gtaagaaatt ccaccgcagc gccgttgaca cgcacgtcga ccaggccggc
      721 gacgtcgtcc gcctcggctt tctgcttcgg ccgcagcttg tcgtcgatgt gcagcgggaa
      781 cccggtccac tcgatctgca ggtgcttgcc gcgccgcgag ggcagcagcg acaggcgctc
      841 gcggctctcc tgccagtgat cgaggtagcg caccaggcgc ccgcgctgcg actcggagac
      901 cagcacgacc tcgagctggc cgtcgcctgc cttgttgtcc ggggcgatgt acaggttggg
      961 gccgatgaag ggcaggttga ccgcctcgaa cagaaggtag cgtcccgaga tgtcgacgcc
     1021 gtcgagctgc gccgcgatct cgaccggctc gtcgcgctcg gccgcttcct tcagccggcg
     1081 cagcgcattg tcgaccacgt gcttcgggcg cttggacttc ttctccttct cgcgcttcgc
     1141 ctggttctcc tcggagcgaa ccagcaggcc ggcgaacagg ccggcgccca ggccctcgac
     1201 gaagtagcgc tccccccacg gcccgcaggc gatgccaacg tcgaggcgca cgcgcttcgc
     1261 gtcgcgccag ccgcgcacca gctcctcgaa cggccgcttg agcaggccga gcgagcgcgc
     1321 gatgttattg gcggttccgg agggcagcac cgccaccggc acaccgcgcc ccaccatgtg
     1381 gcgggtgacg ttcgcaaccg tgccgtcgcc gccggctacg acgaccaagc cggctttctt
     1441 gtcgagcacg cgcgcccagc cccgctcctt cgccgactgg tagcgcggct tgtggccctc
     1501 gcggcgcagc atcttcagca gcgccttggc ggagctcgcg tcctgcttgc ccgcgccggg
     1561 gttgtggatc agcgtgacgc gcacggaaat tgctcgatga aggtcatatg cgcatcctca
     1621 tcaccaacga cgacggcgtc tatagcccgg gcatagccgc tttggcgcag gtggcttccc
     1681 ggttcggcga ggtgcgcatc gtcgcccccg acgtcgagat gtcgtcggcc agccactcga
     1741 tcaccgcttc gcggccggtc acctacaagc gcacgccgct gccaggcctc gacgcctatc
     1801 gcgtcaacgg caccccgggc gactgcgtaa tgctcggcac cacgctctgg gaaaaggtcg
     1861 atctggtcct gtccggaatc aacatcggca ccaacctggg caacgcggtc tggcactcgg
     1921 gaacactcgc cggcgcgaag caggcggcgc tcctcggcct gcgcggcatc gccttcagcg
     1981 ctcccgcgac cgaccgcgag cagccgagct tcgaggcgct taagccttgg gtggccacgg
     2041 tgctggaggc tctgctcgaa gctcccgaca tgccgctcgt caacgtcaac ttccccgcgg
     2101 aggcgccgcg gggcacgcac tggacgcgcc agtccatgag gcactacgac ggcaaggtcg
     2161 ttccagccaa ggatcccatg ggccgtacgc actactggtt caccgtggtg ccggtcgagg
     2221 ccaccgaaga aggcaccgat cgctgggcga tcgaacagcg gtacgtctcg atcactcccc
     2281 tgcgcctcga cctcaccgac caggagaagc ttgccgaagc ccgcaaagtc gagtcctgga
     2341 agaaactgaa gttcgcctag cctagccgcg gttgctaccc gagacgatgc ggaactcgta
     2401 gagccggcac tcgagcgagc cgttccacag cggcgtgcgg cgcgcaggct gcagccggat
     2461 cagcttcgcc atcctcaggt cggcggtgaa gaagaagcag ctccagccgg cgaatttctt
     2521 cttcagcgcg tcgcctagct tcgggtagaa cgcggcgagc tcgtctgcgc tgccgatgcg
     2581 ctcgccgtag ggcgggttgg ccaccatcac cccggcagcc tccggcgccg cacgctccag
     2641 cacgtcggcc tgctcgagct tcacccagcg ctcgacgccc gccgcgccga ggttgcgccg
     2701 cgccgcttgc agcgcctcgg gatcggtgtc gctgccgtat aaccgcggct cgacaggctg
     2761 gaccgtcctc tcgttcttga tcctctccca cagccccgaa tcgaacacgc tcaacctctc
     2821 gaatccgaac gcacgcttcg cgcccggtgc gcggccccgc gccatcgccg ccgcctccga
     2881 gagcagcgtg ccgccgccgc acatcggatc gagcagcggc tggtcgaact tccatcccga
     2941 cagcatgagg atgcccgccg cgaggttctc gcggatcggc gcctccgcga tgtccatccg
     3001 ccatccccgc ttgaacagcg cttcgccgga ggtgtcgagg tacagcgtgc ccttcgcttc
     3061 ctccaggaag gcatgaatgc gcacgtccgg cgccgcgcgg tctacgtcgg gccggctgcc
     3121 gaccttgtcg cggaagagat cgcataccgc gtccttcact ttcagcgtgg cgaactcgag
     3181 gctcttcagc gggcttttct gtgccgtgat gttgaccctg atcttcttct gcacggaaaa
     3241 atactttggc caggcgatcg ctcgcgccgc gtcgtacaga tccttctcgt tccggtaggg
     3301 gaactcgccc acgcgccaga ggatgcgcga agcgatgcgc gaccagaggt tcgcccggta
     3361 gcctatctcc cagcttccgc tgaacgcgac ccctccgggc gcttgcgccg gcgcctcggc
     3421 gccgaacgac gaaagctcct ggagaagcag ctcctccagg ccccgcgggc agctcgcgaa
     3481 gaacgcctca gaacggcttg aagacaacca ggagcaccgt ggcgaacagc accagcgtcg
     3541 ggaactcgtt gaaccagcgg aaccagacgt ggccgcgccg gttgcgctcg gctgcgaagt
     3601 cgcgcatcag ccgccagcac caaaggtgat acccggcgag gagcgccacc agcgcgatct
     3661 tcgcgtgcag ccagccggtg gcaggcgtga accagccgag ccatagccat accccgaaga
     3721 cgatggtgag cacgccaccc ggggtcatga ttccccagaa aagcttgcgc tccatcacct
     3781 tgaagcgctc gagtgagatt ctgtcgtccg cctgcgcgtg gtagacgaag agccgcggca
     3841 ggtagaacag ccccgcgaac caggtcacca tgaagatgat gtgcagcgac ttgacccaga
     3901 gcatggctac gaggaaaagc gcctgcgcgc gaagtagatt gccagcgtgt acccggcggc
     3961 agcatacgcg aggagcacgg cggcgtgcag ccaaaggtcc ggcggcgggc cgcccgcgac
     4021 gagcggccgc acgatctgca cgccgtggta gagcggcagc gccttggcga cggcgaggag
     4081 cggcgcgggt agctgctcgg ccgggaagaa cacacccgag atcatcatca tcggcgtcat
     4141 catgagggtc atgtagtagc tgaagaagtc ccagccgcgc gcgagcacgc acatgatgag
     4201 gccgactgca gagaaggcga ggccgacgag gaaggcggcc gggagggcga gcagcaccca
     4261 gggcgagctc gccagcccga acaccaccac gacgccgagg atggtcgagc cggtcagcca
     4321 ggccttgctc gccgcccaca cgatctcgcc ggcgacgatg tcgtcgatcg tgagcggcgc
     4381 gtagaggatc gcatcccagg tcttctggcc gtgcatgcgc gagaaaccgg agaacatcga
     4441 ctcgaacgag gcggtgaaca tggtcgccgt gcagagctgc ccggcggcga agaacgcgag
     4501 gtacgacatg ccctcgaccg agggcagcag cgcgcccagg ccgtagccca ggccgaacag
     4561 catgatcagc gggtcggcga ggttgctgag gacggccgcg gcgaagagct tgcgccacac
     4621 caacatgttt ctccgccaga ccgagatcca ccgcatctaa tctctcaaat ctcttccggt
     4681 cagtttcagg aagacgtctt cgagactcgc tggacggtgc aggtagctca gctcgccgga
     4741 gatgcttttc agcaccggct cgatgtcttc gccgtagcag tacaccgtgt cgccgacgcg
     4801 ctccacgcgc ggcgcgaggc ccttcgcgcg ctgcatccag gtttcatatc ccgggccgtg
     4861 cacttcgagc acctgcggct cgatgtgctc cgcgatcaag gcgcgcgggg agccttcggc
     4921 gatgatccgg ccgcggtcca tgatcgcgag ccggtggcac aaccgctccg cctcctccat
     4981 gaagtgcgtg gtcaggacga gcgtcttgcc ttcctgcgtc aggcgccgca ggcgctccca
     5041 gatgaggtgg cgcgcctgcg gatcgaggcc ggtggtgggc tcgtccatga acacgagctg
     5101 cgggtcgttg acgagcgcgc gggcgagcgt gaggcggcgc ttcatgccgc cggagagcgt
     5161 agtgatcttg gagtctccgc ggccggcaag cccggcgaac tccagcagcg cggaaatgcg
     5221 ttcccggatt tcttccttgg caatgccgaa gtaccgggca tagaccacca ggttctcttc
     5281 gacggtgaaa tcggggtcga ggttgtcgaa ctgcggcact acgccgacat tccttcgcgc
     5341 ttctcgcgcg cggcgcggca cgggctcgcc tagaagctcg atctcgccgc catcgggctc
     5401 tatcaggccg aggcacagct tgagcgtggt ggtcttgccc gcgccgttcg gcccgagcag
     5461 cccgaagcac tcgccgggcg cgaccgcgag atcgacgccc gagaccacct cgaggtcgcc
     5521 ataagacttg cgcagcgcgc gcgccgacag cgctgtcatt tccagcgggc ttgaacgatt
     5581 cgtttgagga cgtgcagctt gcggtgaaag aaatgatcgg cgccgggcac cagcaccacc
     5641 ggaagctcct gcggccgcgc ccactccagc acttcggcga gcggcaccgt gtcgtcgttc
     5701 tcgccgtgga tcacgagcgt gccttcaggg accggc
//
LOCUS       DATBSZ010000210         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2086099, whole genome shotgun sequence.
ACCESSION   DATBSZ010000210 DATBSZ010000000
VERSION     DATBSZ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086099"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1378)
                     /locus_tag="VIG70_13425"
     CDS             complement(131..1378)
                     /locus_tag="VIG70_13425"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY8251600.1"
                     /translation="MRGSALLLVFVLQACTTSPLTGRDQFLLVSDSTAVSQSAAAYTQ
                     MMADLGKKKQIETGTPRADKVREITDRLVKQAVRVRPDSAKWAWEVQVINDPKTINAF
                     CMAGGKMAIYTGMWDKLKATDDEIAQVMGHEIGHALANHTQERMSVAYGTGIGTTIAA
                     IALGARDSSAVLMQQAAVVAIALPNSRESEAEADQIGIELAARAGYDPKAAVTLWEKM
                     AKVGGKAPPQFLSTHPSPENRAARLKELGEQVQPLYAAAKSGTAVAAPRFLSPPGGGA
                     NERAVTKPGEMTREEYAKLSATDTLTFLSEPFQRFRSGNIVFDCKLQCSFAYSRSKGD
                     WKQLYAAGAWRDLAVSVVQVGYLSDLSYFMLAEAAKGLGMKEAAAAYYKRALEAGKEN
                     ACGSGVFSCEGFEVQKSAATALR"
     gene            complement(1714..>3079)
                     /gene="lon"
                     /locus_tag="VIG70_13430"
     CDS             complement(1714..>3079)
                     /gene="lon"
                     /locus_tag="VIG70_13430"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY8251601.1"
                     /translation="FGLDKIKRRILEHLAVRKLNPEGKSPILCFVGPPGVGKTSLGQS
                     IARATGRKFQRIALGGVHDEAEIRGHRRTYIGALPGNVIQAIRRAESRTCVLMFDEVD
                     KLGQGGFHGDPSSALLEVLDPEQNSHFRDNYLAVDFDLSKVMFITTANMLDTIPGPLR
                     DRMEIIQLPGYTEEEKLEIARRYLVKRQLEANGLTASQVEVGDEAIRKIISDYTREAG
                     VRNLEREIGSVLRNVAMRIAEGEKQKIVVEPKDLHAILGARRFESELAERTSLPGVAT
                     GLAWTSVGGDILFIEASRTPGTGKLILTGQLGDVMKESAQAALTLAKVLTGETLEKTD
                     IHVHVPAGATPKDGPSAGVAMFLSLISLLTSRPVRADVAMTGEISLRGLVLPIGGVKE
                     KTLAALRAGIKTVMLPRRNEKDLEDVPAEARQKLEFVFLDKVEDAIRTAIGELPAPGA
                     RKAA"
BASE COUNT          451 a         1064 c          986 g          578 t
ORIGIN      
        1 tttcatttgc agccgcagtt tctcgccgcc aaacgattat ccgatgccgc ggcgcacgct
       61 ggtatcaagg ggccccgctt cctgctgtcg cgatccgttg aatctggagc ggatcgccaa
      121 cgacaaaccg tcatctcaac gctgtcgccg ccgacttctg cacctcgaac ccctcgcagc
      181 tgaacacgcc gctgccgcaa gcgttttcct tgccggcttc cagcgcgcgc ttgtagtacg
      241 ccgcggcggc ctccttcatg cccaggccct tggccgcctc cgccagcatg aagtacgaga
      301 gatcgctgag gtagcccacc tgcaccaccg acaccgcgag gtcgcgccag gcacctgcgg
      361 cgtacagctg cttccagtcg cccttgctgc ggctgtaggc gaagctgcac tgcagcttgc
      421 agtcgaagac gatattgccg ctgcgaaagc gctggaaggg ctccgagagg aacgtgagcg
      481 tgtcggtcgc cgacagcttc gcgtattcct cacgcgtcat ctcgcccggc ttcgtcaccg
      541 cgcgctcgtt ggcgccaccc cccggcggcg agagaaagcg cggtgccgcg actgcggtgc
      601 cggatttcgc cgcggcatag agcggctgca cctgctcgcc gagctccttc agccgcgcgg
      661 cgcggttctc cggcgaggga tgggtggaaa ggaattgcgg cggcgccttg ccgccgacct
      721 tggccatctt ttcccacagc gtgactgccg ctttcggatc gtagcccgcg cgtgccgcga
      781 gctcgattcc gatctggtcg gcctcggcct cgctctcgcg gctgttgggc agagcgatcg
      841 cgaccaccgc cgcctgctgc atgagcacgg cgctcgagtc gcgcgcacca agcgctatgg
      901 cggcgatcgt ggtgccgatg ccggtgccgt aggctacgga catgcgctcc tgcgtgtggt
      961 tggcgagcgc gtggccgatc tcgtggccca tcacctgcgc gatctcgtcg tcggtcgcct
     1021 tgagcttgtc ccacatgccg gtgtagatgg ccatcttgcc gcccgccatg cagaaggcgt
     1081 tgatggtctt cgggtcgttg atcacctgga cttcccacgc ccacttcgcg gagtcgggcc
     1141 gcacgcgcac cgcctgcttg accaggcggt cggtgatttc cctcaccttg tcggcgcgcg
     1201 gcgtgccggt ctcgatctgc ttcttcttgc cgaggtcggc catcatctgg gtgtaggcgg
     1261 cggccgattg cgacaccgcc gtggagtccg agacgagcag gaactggtcg cggccggtca
     1321 gcggactggt cgtgcaggct tgcaacacaa aaaccaggag gagagccgag ccgcgcatgt
     1381 tgcgatgtcc ctgattcgaa gtgtgatttt acttccgttg tcgcgtccgg cccatcccca
     1441 ggtgattctg caacgcggct ggcttagact tccctccgcc ccgccatgct ccccatccgg
     1501 tcaggccaga agctccccca ggatccggtg ttcaagcatc cgccacagtg gatccacgag
     1561 tccgtggcgg cgaagtgctt ccttgccccc aaggcaaagg acgaagaaag gaaacacccg
     1621 tgttctcccg gcgcgaatcg aaaaaacgcg cgctcgtaag gctgctggac cgtctgccgc
     1681 tttggccggg cttgcgcccg aaacccttgc ccgctaggcc gccttccgcg cccccggcgc
     1741 cggcagctcc ccgatcgccg tcctgatcgc gtcctcgacc ttgtcgagga acacgaactc
     1801 gagcttctga cgcgcttcgg cggggacgtc ttccaggtcc ttttcgttgc gccggggcag
     1861 catcaccgtc ttgatgccgg cgcgcagcgc ggcgagcgtc ttctccttca ccccgccgat
     1921 cggcagcacc agcccgcgca gcgagatctc gccggtcatg gcgacgtcgg ctcgaacggg
     1981 tcttgacgtc aataaagaaa tcaagctcaa gaacattgcc acgcccgcgc tcggcccgtc
     2041 cttcggcgtc gcgcccgccg gcacgtgcac gtggatgtcg gtcttctcca gggtttcgcc
     2101 ggtcaatacc ttcgcgagcg tcagcgcggc ctgcgccgac tccttcatca cgtcgccgag
     2161 ctgcccggtg aggatcagct tgcccgtccc cggggtgcgc gaggcctcga tgaacaggat
     2221 gtcgccgccg accgaggtcc acgcgaggcc ggtcgccacg ccgggcagcg aggtgcgctc
     2281 ggcgagctcg ctctcgaagc gccgcgcgcc caggatggcg tgcaggtcct tcggctcgac
     2341 gacgattttc tgcttttctc cctccgcgat ccgcatcgcc acgttcctga gaaccgaccc
     2401 gatttcgcgc tccaggttgc gcacgcctgc ttcccgcgtg tagtcggaga tgatttttcg
     2461 aatggcctcg tccccgacct ccacctgtga agcggtgagg ccattggcct cgagctgccg
     2521 cttcacgagg tagcgcctgg cgatctcgag cttctcctcc tcggtgtagc cggggagctg
     2581 gatgatctcc atgcggtcgc gcagcggccc gggaatcgtg tcgagcatgt tcgccgtggt
     2641 gatgaacatg accttcgaca ggtcgaagtc caccgccagg tagttgtcgc ggaaatgcga
     2701 gttctgctcg gggtcgagca cctcgagcag cgcgctcgac gggtcgccgt ggaagccgcc
     2761 ctggccgagc ttgtcgacct cgtcgaacat cagcacgcag gtgcgcgact cggcgcggcg
     2821 gatcgcctgg atcacgttgc cgggcagcgc gccgatgtag gtgcggcggt ggccgcggat
     2881 ctcggcctcg tcgtgcaccc cgccgagcgc gatgcgctgg aacttgcgcc cggtggcgcg
     2941 cgcgatcgac tggccgagcg aggtcttgcc gacccccggc gggccgacga agcaaaggat
     3001 ggggctcttg ccctcggggt tgagcttgcg caccgcgagg tgctcgagga tccggcgctt
     3061 gatcttgtcg aggccgaag
//
LOCUS       DATBSZ010000211         2329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_993411, whole genome shotgun sequence.
ACCESSION   DATBSZ010000211 DATBSZ010000000
VERSION     DATBSZ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993411"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VIG70_13435"
     CDS             complement(<1..224)
                     /locus_tag="VIG70_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251602.1"
                     /translation="MSGYSGAPNLEIMEAAVRYNGFLLGLITPLAAGGKRVLDFGAGT
                     GTFAAELQQRKVDIQCVEPDAGLRRALEAKG"
     gene            complement(221..616)
                     /locus_tag="VIG70_13440"
     CDS             complement(221..616)
                     /locus_tag="VIG70_13440"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY8251603.1"
                     /translation="MRAEFAPQLTRFAVVGAIGFVVDATVLQSLVSAAGWSPFAARAV
                     SFPVALACTFALNRAWTFQGLRLPLARAYGAYGAIQIVGALINLCVFSACVLLAPPLY
                     ERPVIALGIGAAVSLLFNFYATRRVVFRT"
     gene            complement(620..1558)
                     /locus_tag="VIG70_13445"
     CDS             complement(620..1558)
                     /locus_tag="VIG70_13445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8251604.1"
                     /translation="MEPSVAVLIPCFNESATIGDVVRSWRGALPAARVYVFDNNSTDD
                     SARLAREAGALVRAEPRQGKGNVVRRMFADVDADLYVLVDGDGTYDASAAPELISRLV
                     AGEFDMVTAARKPVDANSYRPGHRFGNRALTCIVAQVFGKQITDLLSGYRALSRRFVK
                     SFPALSSGFEIETELSVHALELGIPFCEVETRYFARPGGSQSKLSAFRDGWRILMTIV
                     KLVKGERPLAFFTTIAALAAIPALIIGVQLWLTFVETGLVPRFPSAILATGLMLTAFL
                     SLVCGLVLDTVTRGRQEMKRLAYLSWPSFEAAKQRV"
     gene            1599..>2329
                     /locus_tag="VIG70_13450"
     CDS             1599..>2329
                     /locus_tag="VIG70_13450"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY8251605.1"
                     /translation="MPFVPIVLCGGSGTRLWPLSRSHLPKQFLPLISERTMLQETLVR
                     LQGLEDVQAPIAIANDEHRFLAAEQLREISCPPQALLLEPAGRGTAAAAAAAALLLAD
                     KASVMLVLAADHAISNLPQFHAAVRSAVRLAAEGALVVFGIAPASPHTGYGYIKQGAK
                     REDAFVVERFVEKPDLETAKGFLASGGYSWNSGMFAFRADRYLAELGRHRPDIVAAVT
                     KAMAGAQRDADFVRPERAAFLAVPD"
BASE COUNT          384 a          793 c          822 g          330 t
ORIGIN      
        1 cccttcgcct ccagcgcccg ccgcagcccc gcatcgggct cgacgcactg gatgtcgacc
       61 tttctctgct gcaactctgc ggcgaaggtg ccggttcccg cgccgaaatc caggacccgc
      121 ttgcccccgg cggcaagcgg cgtgatgagg cccagcagga agccgttgta gcggacggcc
      181 gcctccatga tttcgaggtt cggggcgccg ctgtagccgc tcacgtgcgg aagaccacgc
      241 ggcgcgtggc gtagaagttg aagagcagcg acaccgcggc gccgatgccg agcgcgatca
      301 ccggccgctc gtacagcggc ggcgcgagca gcacgcaggc cgagaacacg caaaggttga
      361 tcagggcgcc gacgatctgg atcgcgccgt aagcgccgta ggcgcgtgcg agcggcaggc
      421 gcagcccctg gaaggtccag gcgcggttga gcgcgaaggt gcaggcgagc gcgaccggaa
      481 aagacacggc ccgcgcggcg aacggcgacc accccgccgc gctcacgagc gattgcagga
      541 cggtcgcatc gacgacgaag ccgatggcac cgacgacagc gaagcgggtg agctgcgggg
      601 cgaactcggc gcgcaccggc tagacgcgct gcttcgccgc ttcgaacgag ggccagctca
      661 agtacgcgag gcgcttcatt tcctggcgtc cgcgggtcac cgtatccagc accagcccgc
      721 agaccaggct caggaacgcc gtgagcatca ggccggtggc gagaatcgcc gaaggaaagc
      781 gcggcaccag gccggtttcc acgaacgtca gccagagctg cacgccgatg atcagcgcgg
      841 ggatcgcggc aagcgcggcg atggtggtga agaaggcgag cgggcgctcg cctttgacca
      901 gcttgacgat ggtcatgagg atgcgccagc cgtcgcggaa cgccgagagc ttgctctgcg
      961 agccgccggg gcgggcgaag tagcgcgttt cgacctcgca gaaggggatg ccgagctcga
     1021 gcgcgtgcac cgagagctcg gtctcgatct cgaaccccga ggagagcgcc gggaacgact
     1081 tcacgaagcg gcgcgaaagc gcgcggtagc ccgaaagcag gtcggtgatc tgcttgccga
     1141 acacctgggc gacgatgcag gtgagcgcgc ggttgccgaa ccggtggccc ggccggtacg
     1201 aattcgcgtc taccggcttg cgcgccgcgg tcaccatgtc gaactcgcct gccaccagcc
     1261 gcgagatcag ctcgggcgcc gccgaggcgt cgtaggtgcc gtcgccgtcc accaggacgt
     1321 agaggtcggc gtcgacgtcg gcgaacatgc ggcgtaccac gttgcctttc ccctgccggg
     1381 gctcggcgcg cacgagtgcg ccggcctcgc gggcgaggcg cgcgctgtcg tcggtcgagt
     1441 tgttgtcgaa gacgtagacg cgcgccgcgg gcagagcgcc gcgccagctg cggaccacgt
     1501 cgccgatcgt cgcgctttcg ttgaagcacg gaatcaggac cgcgaccgaa ggctccatgg
     1561 ccggcattct aattccagcg ccctataatc ggcccgccat gccattcgta cctatagtgc
     1621 tctgcggcgg atccgggaca cggctgtggc cgctgtcgcg ctcgcatctt ccgaagcagt
     1681 tcctgccgct catttccgag cgcaccatgc tgcaggagac cctcgtacgc ctgcagggcc
     1741 tcgaggacgt gcaggcgccg atcgccattg ccaacgacga gcaccgcttc ctcgccgcgg
     1801 agcagctgcg cgagatctcg tgcccgccgc aggcgctgct gctcgagccc gcgggcagag
     1861 gcaccgccgc cgccgcggcc gcggccgcgc ttctgctggc ggacaaggcg agcgtgatgc
     1921 tggtgctggc cgcggatcac gcgatttcga atctgccgca gttccacgcc gcggtgcgct
     1981 cggccgtgcg gcttgccgcc gagggcgcgc tcgtcgtgtt cggcattgcg ccggcaagcc
     2041 cgcataccgg ctacggctac atcaagcagg gcgcgaaacg cgaggatgcc tttgtcgtcg
     2101 agcgcttcgt ggagaagccg gacctggaaa ccgcaaaggg attcctcgca tcgggcggtt
     2161 actcctggaa tagcggcatg ttcgcgttcc gcgccgatcg ctacctcgcc gagctcgggc
     2221 gccaccggcc cgatatcgtc gcggccgtca ccaaggcgat ggcgggcgcg cagcgcgacg
     2281 ccgatttcgt gcgcccggag cgcgcggcgt tcctcgcggt tcccgacga
//
LOCUS       DATBSZ010000212         6703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2018072, whole genome shotgun sequence.
ACCESSION   DATBSZ010000212 DATBSZ010000000
VERSION     DATBSZ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018072"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1060
                     /locus_tag="VIG70_13455"
     CDS             71..1060
                     /locus_tag="VIG70_13455"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251606.1"
                     /translation="MKRRQFLKSTAAIAGTAGLGFPEKGFSQAMPDVIHVGHLVGICM
                     SPLFYAQATGLFAAEGIKVDMKFMPNPGDALTALTGGVMQVVHIPFTNIIVAANNGAP
                     VRIIGGSGAGGLFLLAQGSSGIKSMDDLKKQKGKGLKIGAMRLNTFELMAYRALANAG
                     LKYEDFQMVWFSDTLSMASAFEAKAVDLVTHVEPFATRLVDKLGGVPIASNLDAWGKD
                     GPDCVTNARVDFIEKYPAAVTKYLRALLKADAAIKADPAKAVDVLDASKFYRVDKDTL
                     RAALPRQRPQVNLIGAGEAGMRTAIADMTTLGYIKSVPANLIDFRFLKEALKA"
     gene            1069..1842
                     /locus_tag="VIG70_13460"
     CDS             1069..1842
                     /locus_tag="VIG70_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8251607.1"
                     /translation="MARAAAAALIPWLILVALWELASARGWMTTVLFPPPSSFIRFAI
                     ESDFRVGFGQEGMTIPVAILASAFRVVAGLFLGFIAAVATGILVSMSRAASAAVLPIV
                     RGLAPIAPIAWIPVGIIVFGVGNPTAVFVVFTGVYFLLTLSTVAAVQSVDPRLVKTAR
                     TFGASRAQVWAWVIFPAVLPQVFTMLRINFFAAWMAVLAAEMVGLKNGVGMIVILGRE
                     MFNPNLIMLGICIIGVTGYAVDALLLFVQRQILWWKSAE"
     gene            1973..2746
                     /locus_tag="VIG70_13465"
     CDS             1973..2746
                     /locus_tag="VIG70_13465"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251608.1"
                     /translation="MSSLQIRNVRKIWQGARGLTVEAIRDVSLQVSSGEFVAILGPSG
                     CGKSTLLELCAGLEPASGGEILIGAERVTGPNPKAVMVFQEHSLFPWLDVEKNVAFGL
                     QMRGTGLNERWRRGREVLERVKLVKFAKHYPHQLSGGMKQRVAIARALVGNPEFILMD
                     EPFAALDFQTRVLMQQFLLEVWAGFKSTILFVTHHIDEAILLADRVVVMSSAPGRVLE
                     EVQIDLPRPRSMTDPDFNGYRVRLTSHMEREVMRAAEVE"
     gene            2751..3053
                     /locus_tag="VIG70_13470"
     CDS             2751..3053
                     /locus_tag="VIG70_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251609.1"
                     /translation="MKWSDLFDGLRYSARCGELSGSVVEIAGYVVDTHDGSAQLLVAE
                     PGGCPHCSPVPAVSLPDFRMARKGEAVRLRGTLSYGFKVDREGAGSFLRLENAHLA"
     gene            3050..4498
                     /locus_tag="VIG70_13475"
     CDS             3050..4498
                     /locus_tag="VIG70_13475"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY8251610.1"
                     /translation="MILIQNALVLTPSGEGQPLDVLVDGGTIADVVPRGSLKSEGMER
                     VDAAGRALMPGLVNGHNHAQTGLSKGLFDRFNLETYLNAQPGVSGRRTLEDKHLSALI
                     GAAEMVRKGCTAGYDMFAEFPLPTAEGVEAVARAYSDAGMRAAIAPMMADKTFYEAIP
                     GLAEALPEPLRSQALKAKYAPYPETLSSVRNLLNHWKFDRDRIKPALGPTIPHHCSDE
                     FLVACRDLAGELGLGIQMHVAESRLQAAVAPKIYGKSLVAHLHELKLLNPKFCVAHGV
                     WLDDDDRKRLADSGASISHNPGSNLKLGSGIADMRAMLARGLNVAIGTDGAASADNLN
                     AFEAMRLACYLSRVKGNPPERWVNAREALHAATEGGAKALGFDKLGRIEKGWKADLVL
                     LDLGALHYIPLNDLASQIVFAEDGTGVENVMIGGRWVMRDRKLLTVDVESLRRRADEA
                     VARLRAANAEARALGEKLHPYVGHFCAGLTPG"
     gene            complement(4495..4842)
                     /locus_tag="VIG70_13480"
     CDS             complement(4495..4842)
                     /locus_tag="VIG70_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251611.1"
                     /translation="MSIRRRRSKALAAQTVDLAFAVPQVVAHRMARMAMAGGTPSARD
                     RREFYRMGTEKVAAFNEAWAAMAMQTWRSFWFPTHLSSAALDILGKGMTPYRRRAVAN
                     ARRLGRPKRRRRR"
     gene            complement(4937..5860)
                     /locus_tag="VIG70_13485"
     CDS             complement(4937..5860)
                     /locus_tag="VIG70_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8251612.1"
                     /translation="MASEIRRKIVCAALSGALPWLAPAARSAEGPAATRPIPSSGERL
                     PIVGMGTAVIFDYQNDPAKQAERKEVIRTLIAGGGRLIDTAAGYGNAEERLGEIVADL
                     GVRGKLFLATKFSFDLPRAEAEASFRSSLRRLRTDRVDLMQAWNVTEAGYTLDLLREW
                     KKAGLCRYIGITTSQVRSYDAMAKVVAREKPDFFQVNYSLGALESEKSLLPAARDAGA
                     AVLVNLPFGRNSLFRKAGNRPLPDFAAEFGARTWAQFFLKFILGHPAVTAVIPGTDKP
                     EYMLDNLDAARGTLPDAAMRKRIVQYWDSLT"
     gene            complement(5850..>6703)
                     /locus_tag="VIG70_13490"
     CDS             complement(5850..>6703)
                     /locus_tag="VIG70_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8251613.1"
                     /translation="VRTARPALAQDRIMTKKIPSTGEPLPVIGLGTWQTFDVGADAAG
                     RAPLREVLKLLDRNVVDSSPMYGSSETVAGDLIAEAGMREKLFMATKVWTSGRDAGIR
                     QMETSFKRLRVERMDLMQVHNLVDVDTHTKTLQDWKAKKRVRYIGITHYTSSAYAEVE
                     RQMKKAQYDFLQINYSLGERESENRVLGLAKDRNMAVIINRPFAEGALFRRTKNQPLP
                     PWAAEFGIESWAQYFLKWIVSHPVVTCAIPGTGNPKHMTDNLGAGRGALPDEAGRKRM
                     AAYFDGL"
BASE COUNT         1109 a         2192 c         2256 g         1146 t
ORIGIN      
        1 gattaaatag cgcttccaag cctggcacgg gtcgtgcgag acgcacagac caccaacaaa
       61 ggggagagcc atgaagcgcc gccagttcct gaagtccact gccgccatcg ccggcaccgc
      121 agggctaggt tttccggaga agggtttttc ccaggcgatg cccgacgtca tccacgtggg
      181 ccacctggtc gggatctgca tgtcgccgct cttctatgcg caggccacgg gcctgttcgc
      241 ggcggagggg atcaaggtgg acatgaagtt catgccgaac ccgggcgacg ctctcaccgc
      301 gctgaccggc ggggtgatgc aggtcgtgca catccctttc accaacatca tcgtcgcggc
      361 gaacaacggc gcgccggtgc gcatcatcgg cggcagcggc gccgggggcc tgttcctgct
      421 tgcacaggga agctcgggca tcaaatcgat ggatgacttg aaaaaacaaa aaggaaaggg
      481 cctgaagatc ggcgcgatgc gcctcaacac gttcgagctg atggcctacc gcgcgctggc
      541 gaacgcgggg ctgaaatacg aggacttcca gatggtgtgg ttcagcgaca cgctgtcgat
      601 ggcctcggcg ttcgaggcga aggcggtgga cctcgtcacg cacgtcgaac ccttcgccac
      661 gcgcctggtc gacaagctgg gcggggtgcc gatcgcctcc aacctcgacg cgtggggcaa
      721 ggacggcccg gactgcgtca ccaacgcgcg cgtcgacttc atcgagaagt acccggccgc
      781 ggtgacgaag tacctgcggg cgctcctcaa ggccgacgcg gcgatcaagg cggatcccgc
      841 caaggcggtc gacgtgctcg acgcctcgaa gttctaccgc gtcgacaagg acacgctgcg
      901 cgccgcgctg ccgcgccagc ggccgcaggt gaacctgatc ggcgccggcg aggcgggcat
      961 gcgcacggcg atcgccgaca tgactacgct cggctacatc aagtcggtgc cggcgaacct
     1021 gatcgacttc aggttcctga aagaagcgct gaaggcgtag cggcaggaat ggccagagcg
     1081 gcggccgcgg ccctcatacc gtggctgatc ctggtcgcgc tgtgggagct cgcgtcggcg
     1141 cgcggctgga tgacgacggt gctgttcccg ccgccatcgt cgttcatccg ctttgcgatc
     1201 gagagcgact tccgcgtcgg cttcggccag gaggggatga cgatccctgt tgcgatcctg
     1261 gcgagcgcgt tccgcgtggt ggcgggcctg ttcctcggct tcatcgccgc ggtggccacc
     1321 ggcatcctgg tctccatgtc gcgcgccgcg tcggcggccg tgctgccgat cgtgcgcggc
     1381 ctggcgccaa tcgcgccgat cgcctggatc ccggtcggca tcatcgtctt cggcgtcggc
     1441 aatcccaccg cggtgttcgt ggtgttcacc ggggtgtact tcctgcttac gctctccacc
     1501 gtggcggcgg tgcagtcggt cgacccgcgc ctggtcaaga ccgcgcgcac cttcggcgcc
     1561 tcgcgcgccc aggtgtgggc ctgggtgatc tttccggcgg tgctgccgca ggtgttcacc
     1621 atgctgcgca tcaacttctt tgccgcctgg atggcggtgc tcgccgccga gatggtgggc
     1681 ctcaagaacg gcgtcggcat gatcgtgatc ctcggccgcg agatgttcaa cccgaacctg
     1741 atcatgctcg gcatctgcat catcggcgtc accggatacg ccgtcgatgc tttactgctt
     1801 tttgttcaac gacagattct gtggtggaag tccgcagagt gacctgccgg gtttgccccc
     1861 ctgacaaggg gggaggcgag cgcgtttttc gcgagctggg gggttgtttt tttcaaacac
     1921 aaaccccccg ctcgccttcg gctcacgccc cccttgtcag ggggccaaag caatgagctc
     1981 gctgcagatc cgcaacgtga ggaagatctg gcagggcgcg cgtggcttga cggtggaggc
     2041 gatccgcgac gtcagcctcc aggtctcctc gggcgagttc gtcgccatcc tcggcccgag
     2101 cggctgcggc aagagcacgc tgctcgagct gtgcgcgggc ctcgagccgg cgtcgggcgg
     2161 cgagatcctg atcggagccg agcgcgtcac cggcccgaat cccaaggcgg tgatggtgtt
     2221 ccaggagcat tcgctctttc cgtggctcga cgtggagaaa aacgtcgctt tcggcctgca
     2281 gatgagggga accggcctga acgagcgctg gcgccgcggg cgcgaagtac tcgaacgcgt
     2341 gaagctggtc aagttcgcca agcactaccc gcaccagctc tccggcggca tgaagcagcg
     2401 cgtggcgatc gcccgggcgc tagtcggcaa cccggagttc atcctgatgg acgagccgtt
     2461 cgcggccctc gacttccaga cgcgcgtgct gatgcagcag ttcctgctcg aggtgtgggc
     2521 cgggttcaag tcgacgatcc tcttcgtcac gcaccacatc gacgaggcga tcctgctcgc
     2581 cgaccgcgtg gtggtgatga gctccgcgcc cgggcgcgtg ctcgaggagg tgcagatcga
     2641 cctgccgcgg ccgcgcagca tgaccgaccc ggacttcaac ggctaccgcg tgcgcctgac
     2701 gtcgcacatg gagcgcgagg tgatgcgtgc ggccgaagtt gagtgagcgt gtgaaatgga
     2761 gtgacctctt tgatggactg aggtacagcg cgcgctgcgg cgagctctcg ggaagcgtgg
     2821 tcgagatcgc gggctacgtc gtcgacacgc acgacggcag cgcgcagctc ctggtggccg
     2881 agccgggcgg ctgcccgcac tgctcgccgg tccctgctgt ctcgcttccc gactttagaa
     2941 tggcaagaaa aggagaagcg gtgagactgc gcggcacgct gtcgtacggc ttcaaggtcg
     3001 accgagaagg cgcgggctcg ttcctgcgcc tggagaacgc acacctcgca tgatcctcat
     3061 ccagaacgcg ctggtgctga cgccgtcggg cgaagggcag cccctcgacg tgctggtcga
     3121 cggcggcacg atcgccgacg tcgtgccccg cgggtcattg aagagcgagg gcatggaacg
     3181 cgtcgatgcc gccgggcgcg cgctgatgcc cgggttggtg aacggccaca accacgcgca
     3241 gaccggcctc tcgaagggac tcttcgaccg cttcaacctc gagacctatc tcaacgccca
     3301 gcccggcgtg agcggccggc gcacgctcga ggacaagcac ctctcggcgc tgatcggcgc
     3361 cgccgagatg gtgcgcaagg ggtgcaccgc cggctacgac atgttcgccg agttccccct
     3421 gcccacggcc gagggcgtgg aggcggtggc gcgcgcctac tccgacgccg gcatgcgcgc
     3481 cgcgatcgcg ccgatgatgg cggacaagac cttctacgag gcgatccccg ggctcgccga
     3541 ggcgctgccg gagccgctgc gcagccaggc gttgaaggcc aaatatgcac cgtatccgga
     3601 aacgctctct tcggtccgca atctcttgaa ccactggaag ttcgaccggg accgcatcaa
     3661 gccggcgctg ggccccacca tcccgcacca ttgctccgac gagttcctgg ttgcgtgccg
     3721 cgacctcgcg ggcgagcttg gcctcggcat ccagatgcac gtcgcggaat cgaggctgca
     3781 ggcggcggtc gcgccgaaga tctatggcaa gagcctcgtc gctcacctcc atgagttgaa
     3841 attgctgaac ccgaaattct gcgtcgccca cggcgtctgg ctcgacgacg acgatcgcaa
     3901 gcgcctcgcc gactccggag cctcgatctc gcacaacccg ggaagcaatc tcaagctcgg
     3961 ctcgggaatc gccgacatgc gcgcgatgct cgcgcgaggc ctgaacgtcg ccatcggcac
     4021 cgatggcgcg gcttccgcag acaacctcaa cgccttcgag gcgatgcgcc ttgcttgcta
     4081 tctatcacgc gtcaagggta atccacccga gcgttgggtg aacgcgcgag aggcgctcca
     4141 tgcggccacg gagggcggcg cgaaagcgct cggcttcgac aagctcggcc gcatcgagaa
     4201 aggctggaaa gccgacctcg tgctcctcga cctgggcgca ttgcactaca tccccctgaa
     4261 cgacctcgcg agccagatcg tcttcgccga ggacggcacc ggagtggaga acgtgatgat
     4321 cggcgggcgc tgggtgatgc gcgatcgcaa gctcctgacc gtggacgtcg agtcgctcag
     4381 gcgcagggcg gatgaagcag tcgctcgcct gagggcagcg aacgccgaag caagagcgct
     4441 cggcgaaaag ctccatccct acgtcgggca cttctgcgcc ggactaactc caggctaacg
     4501 gcgtcgcctt cgcttggggc ggccgagacg cctggcattc gcgaccgcgc gacggcgata
     4561 gggcgtcatg cccttgccca gaatgtccag cgcggcgctg ctcaggtgcg tgggaaacca
     4621 aaacgaccgc caggtctgca tggccatcgc ggcccaggct tcgttgaagg ccgcgacctt
     4681 ctccgtgccc atgcgataga actcgcggcg gtcccgcgcc gaaggagtcc ccccggccat
     4741 ggccattcgg gccatccgat gcgcgaccac ctgcggcacg gcaaaggcaa ggtcgactgt
     4801 ttgtgcggcg agtgctttgg acctgcggcg gcgaatggac atggactcat atagcacggc
     4861 caagtccgcg tccgctgtag tcaaaaccgc gattcgcagg ccctgcgccg ctagccggcc
     4921 cgcgaggcaa gacgaatcag gtgagcgaat cccagtactg aacgattctc ttccgcatcg
     4981 ccgcgtccgg aagcgtgccg cgggcggcgt cgaggttgtc gagcatgtac tccggcttgt
     5041 ccgtgccggg aatgacggcg gtgaccgcgg ggtgcccgag gatgaacttg aggaagaact
     5101 gcgcccaggt ccgggcgccg aactcggcgg cgaaatccgg caatggacgg ttgcccgcct
     5161 tgcggaacag cgaattgcgg ccgaacggca ggttcacgag caccgccgct cccgcgtcgc
     5221 gcgcggcggg caggagcgat ttctcggatt cgagcgcgcc gagcgaatag ttcacctgga
     5281 agaaatccgg cttctcccgc gcgacgacct tcgccatcgc gtcgtagctg cgcacctggc
     5341 tggtggtgat gccgatgtag cggcaaaggc ccgctttttt ccattcccgc agcaggtcga
     5401 gggtgtagcc agcctcggtg acgttccacg cctgcatcag gtcgacgcgg tcggtgcgca
     5461 ggcggcgcaa cgagctgcga aatgacgcct ccgcttccgc gcgcggcagg tcgaaggaga
     5521 acttcgtggc gaggaagagc ttgcccctga cgcccaggtc cgcgacgatt tcccctagcc
     5581 gctcttctgc gttgccgtag cctgccgccg tgtcgatcag gcggccgccg ccggcgatga
     5641 gcgtgcggat cacctccttg cgctccgcct gcttcgcagg atcattctgg tagtcgaaga
     5701 tcaccgccgt gcccatgccg acgatcggca gccgctcgcc actcgagggg atcggccgcg
     5761 tggcggccgg cccctcggcg ctgcgcgctg caggggcgag ccatggcagc gcgcccgaaa
     5821 gcgccgcaca gacgatcttg cggcggatct cagaggccat cgaagtaggc ggccatgcgc
     5881 tttcttcccg cttcgtcggg cagggcgcca cggccggcgc ccaggttgtc ggtcatgtgc
     5941 ttcgggttgc cggtgccggg gatggcgcag gtcaccaccg ggtgcgacac gatccacttg
     6001 aggaagtact gcgcccagct ctcgatgccg aattccgctg cccacggtgg aagcggttgg
     6061 ttcttcgtcc gtctgaacag agcaccttcg gcgaacgggc gattgatgat gaccgccatg
     6121 ttccggtcct tggccaaacc caacacccgg ttttcggatt cgcgttcgcc caacgagtag
     6181 ttgatctgca ggaagtcgta ctgcgccttc ttcatctggc gctcgacctc ggcgtaggcg
     6241 gaggacgtgt agtgcgtgat gccgatgtag cgcacgcgct tcttcgcctt ccagtcctgc
     6301 aaggtcttcg tgtgcgtgtc gacgtcgacc aggttgtgca cctgcatgag atccatgcgc
     6361 tcgacgcgca ggcgcttgaa ggaggtctcc atctgccgga tgccggcgtc gcggccgctg
     6421 gtccagacct tggtggccat gaagagcttt tcgcgcatac ccgcctcggc gatgaggtcg
     6481 ccggcgaccg tctcggagct gccgtacatg ggcgaggaat cgaccacgtt ccggtccagc
     6541 agcttcagca cctcgcgcaa tggcgcgcgg ccggccgcat cggcgccgac gtcgaacgtc
     6601 tgccacgtgc cgaggccgat gacgggaagc ggctcgccgg tcgagggaat cttcttggtc
     6661 atgatgcggt cctgtgcaag cgcgggtcgt gcggttctga ctg
//
LOCUS       DATBSZ010000213         5735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2292543, whole genome shotgun sequence.
ACCESSION   DATBSZ010000213 DATBSZ010000000
VERSION     DATBSZ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2292543"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1557)
                     /locus_tag="VIG70_13495"
     CDS             complement(52..1557)
                     /locus_tag="VIG70_13495"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEY8251614.1"
                     /translation="MAANPLRALGSPTPHPFEAIDYPAVPAKPRTTAVTSVIDGGLGP
                     AEARDRVAIAAEWIDVVKLGWATARLTPGATLRDKVQIYRAAGIHTCSGGTFLEIAFA
                     QNRVREFLDGARDLGLTMVEVSNGVHPMSEEDKLGLIAQARSAGFTVWSEVGKKDPDE
                     DARIGIDDRCAAIARELEAGANKVILEARESGTVGIYDRSGSPAVEMIQRIVESVGRE
                     RLVFEAPRKEQQLWMIRALGSDVNLGNVAPEEALSLATLRTGLRGDTFREMHLHGLEI
                     FLGQGVHGALEARARGGVIVMIDALRASATMIAALEMGMAAVKPVASAEECTGEVTAG
                     ERGGRKLPNCRHNNSPLELMRHRYAGKTLVFTSTNGAECLLSAAGPESIVLVGSSLNR
                     RAVAGAAGRLARKRGVPITLLMAGRNNQYAVEDALAAGEIARALPEARLHGELPSTAA
                     LEADFCASDSGRNLIALGYADDVRFCAQLDRCEVVPVLMNGQLVPLRREHE"
     gene            complement(1687..2934)
                     /locus_tag="VIG70_13500"
     CDS             complement(1687..2934)
                     /locus_tag="VIG70_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8251615.1"
                     /translation="MKRRDFLKLAGAVSLAGCATATPSKARVVVIGGGFGGATAAKYI
                     RLWDPSIDVVLVERDASFVSCPISNLVLGGFTGMADITRGYDGLQRHGVQVVRDEATA
                     VDPSKKVVRLARGGDIAYERLIVSPGIDFTFGDVAGYEAAMQDGTVLHAWKAGPQTVA
                     LRRQLEEMRDGGIYILSVPLAPYRCPPGPYERASMVAAYFKQAKPRSKVLVLDANPDV
                     TSKGGLFKRAWSDLYAGILEFRGNSRAVAVDGKSRTVKLELEDVKGDVLNVVPPHRAG
                     DIAMKTGLITTNNRWCDVDWRTMESKAVKGVHVLGDATLSASGMPKSGSMANNHAKIA
                     AAAIVELLNGRAPQPVKIINTCYSFVSQKEGIRVSSVHEWDAGQGTLVPVKGSGGVSA
                     ARSEAEGTYAWSWARTIWADSLA"
     gene            complement(2940..3233)
                     /locus_tag="VIG70_13505"
     CDS             complement(2940..3233)
                     /locus_tag="VIG70_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8251616.1"
                     /translation="MSRSIVFAAALAAALSAHAQESGRNLAAGCAICHGTDGKAVTKD
                     VIPLAGLPREHIATQMRAFRDGQRPATVMHQIAKGYSDAQIDAMAAWFAAQKR"
     gene            complement(3333..5597)
                     /gene="clpA"
                     /locus_tag="VIG70_13510"
     CDS             complement(3333..5597)
                     /gene="clpA"
                     /locus_tag="VIG70_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEY8251617.1"
                     /translation="MIAQELEVSLHMAFVEARQKRHEFITVEHLLLALLDNPTAAEVL
                     RACGANMDELRKNLTQHITEQTPRIAADREVDTQPTLGFQRVIQRAILHVQSSGKKEV
                     TGANVLVAIFGEKDSHAVYFLQQQGIARLDVVNYISHGITKTPQAHQGKSADQDAEQE
                     NQAETSNSPLDNYTQNLNALALAGKIDPLIGRDRELERVVQTLCRRRKNNPLLVGEAG
                     VGKTAIAEGLARRIVEGNVPDLLTKSTVYVLDMGALLAGTKYRGDFEQRLKAVLKQLV
                     DNPNAILFIDEIHTVIGAGAASGGTLDASNLLKPVLSTGQLKCIGATTYNEYRGVFEK
                     DHALSRRFQKIDVVEPSVDETVEILKGLKSRFEAHHSVKYTANALTTAAELSARYIND
                     RHLPDKAIDVIDEAGAAQRIAPKAKQKKIIGKSEIEEIIAKIARIPPRSVSSDDRSAL
                     ANLDRDLKAVVFGQDQAIDALSSAIKMARSGLGNPQKPIGNFLFSGPTGVGKTEVARQ
                     LAYCMGIELIRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEQITKKPYSVLLLDE
                     IEKAHPDIFNVLLQVMDHGTLTDNNGRKADFRNVCIVMTTNAGAESLARTQMGFTPSV
                     KMGDEMEAIKRMFTPEFRNRLDAIISFRALDHEIILRVVDKFLMQLEEQLAEKKVEAQ
                     FTAELKLHLAKKGFDPLMGARPMARLIQDTVRRALADELLFGRLQHGGKVTIDVGEDE
                     KITLKFEEEPAAVA"
     gene            complement(5602..>5735)
                     /locus_tag="VIG70_13515"
     CDS             complement(5602..>5735)
                     /locus_tag="VIG70_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEY8251618.1"
                     /translation="KVHREGIGVCGVYPRDVASTKVQQVAAYSRKHQHPLQCVMEET"
BASE COUNT          852 a         1909 c         1924 g         1050 t
ORIGIN      
        1 gccgccagcg cgcgcggtac cggcggcttc ggctcgaccg ggaaagccta gctactcgtg
       61 ctcccgccgc agcggcacga gctgcccgtt cattaggacc gggacgacct cacagcggtc
      121 cagctgcgcg caaaagcgca catcatcggc gtaacccagg gcgatcaggt tgcggcctga
      181 atcgctggcg cagaagtcgg cttcgagcgc cgccgtcgac ggcagctcgc cgtgcaaacg
      241 cgcctccggc agcgcacgcg cgatctcgcc ggcggcgagg gcgtcttcta cggcgtactg
      301 gttgttgcgc cccgccatga gaagggtgat cgggacgccg cgcttgcgcg ccagtcgccc
      361 cgccgcgccc gcgaccgcgc gccggttgag gctcgagccc acgagaacga tgctctccgg
      421 ccccgccgcg cttagcaggc actcggcgcc gttggtcgag gtgaagacca gggtcttgcc
      481 cgcgtagcgg tgccgcatga gctcgagcgg gctgttgttg tgccggcagt tgggcagctt
      541 gcgtccgccg cgctcgccgg cggtcacttc tccggtgcat tcctcggcgc tggcgactgg
      601 cttgaccgcc gccatgccca tctcgagcgc tgcgatcatg gttgcgctcg cccgcagcgc
      661 gtcgatcatg acgatgacgc cgccacgcgc tcgcgcctcg agcgcgccgt gcactccctg
      721 accgaggaag atttccaggc cgtgcaggtg catctcgcgg aacgtgtcgc cgcgcaggcc
      781 ggttcgcagc gtcgcgagcg acagagcctc ttcaggcgcc acgttgccga ggttcacgtc
      841 ggatccgagg gcgcgaatca tccagagctg ctgctccttg cgcggcgcct cgaacaccag
      901 ccgctcgcgt ccgacgctct ccacgatccg ctggatcatc tcgaccgccg ggctgccgct
      961 gcggtcgtag atcccgacgg tgcccgattc gcgcgcctcg aggatgacct tgttcgcacc
     1021 ggcttccagc tcccgggcga tcgcggcgca acggtcgtcg atgccgatcc gggcgtcctc
     1081 gtccggatcc ttcttgccca cctcgctcca cacggtgaac cccgccgagc gggcctgggc
     1141 gataagcccg agcttgtcct cttcgctcat gggatgcacg ccgttcgaca cttcgaccat
     1201 ggtcaagccg agatcgcgcg cgccgtccag aaattcgcgc acgcggttct gggcgaacgc
     1261 gatctcgagg aaggtgccgc cgctgcatgt atggattccg gccgcgcgat agatctggac
     1321 cttgtcgcgc aaggtagcgc ccggagtaag ccgcgccgtt gcccatccga gcttcaccac
     1381 atcgatccac tcggcggcaa tcgcgacgcg gtcgcgggcc tcggcgggcc ccagcccccc
     1441 gtcaatcacc gacgtgacgg cggtcgtgcg cggcttggcg ggaacggcgg ggtaatcgat
     1501 cgcctcgaac ggatgtggag tcggggaccc taaagctcgt aatggattgg cggccatcgg
     1561 ttcccaagcc tgccataggc tcgcgtctcc cttgatgcct tagaccaccg gcgtaaagga
     1621 cttttacaga gaaccgtcaa atactgtggg ctggcgcctt actcgagcga agagcggact
     1681 gcaatatcag gctagcgagt ccgcccagat cgttcgggcc cagctccagg cgtaggtccc
     1741 ctcggcctcg ctgcgcgcag cggaaacgcc acccgagccc ttcaccggca ccagcgtgcc
     1801 ctggccggcg tcccattcgt gcaccgacga gacgcgaatc ccttccttct gcgacacgaa
     1861 gctgtagcag gtgttgatga tcttcaccgg ctgcggcgcg cggccattga gcagctcgac
     1921 gatcgcggcg gcggcgatct tggcgtggtt attcgccatg ctgcctgact tcggcattcc
     1981 ggaagcggaa agcgtggcgt cgccgagcac gtgcacgccc ttgaccgctt tcgactccat
     2041 ggtgcgccag tcgacgtcgc accagcggtt gttggtggta atgagcccgg tcttcatggc
     2101 gatgtcgccc gcacggtgcg gcggcaccac gttgaggacg tcacctttca cgtcctcgag
     2161 ctcgagcttg acggtccgcg acttcccgtc caccgcaacg gcgcgcgagt tgccgcggaa
     2221 ttcgaggatc cccgcgtaga ggtcgctcca ggcgcgcttg aacaacccgc ctttcgaggt
     2281 gacgtcggga ttggcgtcga gcaccagcac cttggagcgt ggcttcgcct gcttgaagta
     2341 ggccgcgacc atgctcgcgc gctcgtacgg cccgggcggg cagcggtagg gcgccagcgg
     2401 caccgagaga atgtagatgc cgccgtcgcg catttcctcg agctggcgac gcagcgccac
     2461 ggtctgcggc ccggccttcc aggcatgcag caccgtgccg tcctgcatgg cggcctcata
     2521 tcccgcgacg tcgccgaagg tgaagtcgat tcccggcgac acgatcaagc gctcgtaggc
     2581 gatatcgccg ccacgcgcca gccggacgac ctttttcgaa ggatcaacgg cagtggcttc
     2641 gtcgcgcacc acttgcacgc cgtggcgctg caggccgtcg tagccgcgag tgatgtcagc
     2701 catgccggtg aagccgccga gcaccaggtt ggagatcggg caggagacaa aggaagcgtc
     2761 gcgctcgacc agcactacgt cgatcgatgg gtcccacagg cggatgtact tcgccgcggt
     2821 ggcgccgccg aagccgccgc cgatcaccac cacgcgggcc ttggaaggcg tcgcggtcgc
     2881 gcagccggcg agggacaccg cgccggcaag cttcaggaag tcgcgtcgtt tcatggcgct
     2941 cacctcttct gagcggcaaa ccacgccgcc atggcatcga tctgggcgtc gctgtagccc
     3001 ttggcgatct ggtgcatcac cgtcgccggt cgctgaccgt cgcggaacgc gcgcatttgc
     3061 gtggcgatgt gctcgcgcgg cagtccggcg agcgggatga cgtccttggt gacggccttg
     3121 ccgtcggtgc cgtggcagat ggcgcagccg gcggcgaggt tgcggccgct ttcctgggca
     3181 tgcgcggaaa gcgcagcggc gagcgcggcg gcgaaaacga tgcttcgact catgaacccc
     3241 tcccttgggg tccagaaagt aatcccgcac cccggaaaaa gcaaaggccg ctcgggggcg
     3301 gcctcgtgtc ttcggcaatt attggatagg cgtcaggcca ccgcggccgg ctcttcctcg
     3361 aacttgagcg tgatcttctc gtcctcgccg acgtcgatgg tcaccttgcc gccgtgctgc
     3421 agccgtccga acaggagctc gtccgccaac gcgcggcgca ccgtgtcctg gatgaggcgc
     3481 gccatcgggc gcgcgcccat cagggggtcg aaccccttct tcgccaggtg cagcttgagc
     3541 tcggcggtga actgcgcctc gaccttcttc tcggcgagct gctcctcgag ctgcatcagg
     3601 aacttgtcga ccacacgcag gatgatctcg tggtcgagcg cgcggaacga gatgatcgcg
     3661 tcgagccggt tcctgaactc gggcgtgaac atgcgcttga tcgcctccat ctcgtcgccc
     3721 atcttgaccg acggggtgaa gcccatctgc gtgcgcgcca gcgactcggc gccggcgttg
     3781 gtggtcatga cgatgcacac gttcctgaag tcggccttcc tgccgttgtt gtcggtcagg
     3841 gtgccatggt ccatcacctg caggagcacg ttgaagatgt ccgggtgcgc cttctcgatc
     3901 tcgtcgagca gcagcaccga gtagggcttc ttggtgatct gctcggtgag caggccgccc
     3961 tgctcgaagc cgacgtatcc cgggggcgcg ccgatgagcc ggctcaccgc gtggcgctcc
     4021 atgtactccg acatgtcgaa ccggatgagc tcgattccca tgcagtacgc gagctgccgg
     4081 gccacctcgg tcttgccgac cccggtgggg ccggagaaca ggaagttgcc gatcggcttc
     4141 tgcgggttgc cgagccccga gcgcgccatc ttgatcgccg aggagagggc gtcgatggcc
     4201 tggtcctggc cgaacacgac cgctttcagg tcgcggtcca ggttggcgag cgcgctgcga
     4261 tcgtcgctcg acacgctgcg cggcgggatg cgcgcgatct tggcgatgat ctcctcgatc
     4321 tcggacttgc cgatgatctt cttctgcttg gccttgggcg cgatgcgctg cgcggcgccg
     4381 gcctcgtcga tcacgtcgat cgccttgtcg ggcaggtggc ggtcgttgat gtagcgcgcg
     4441 gagagctcgg ccgcggtggt gagcgcattg gcggtgtact tcacgctgtg gtgggcctcg
     4501 aagcggctct tgaggccctt caggatctcg accgtctcgt cgaccgaggg ctcgaccacg
     4561 tcgatcttct ggaagcgccg cgacagcgcg tggtccttct cgaacacgcc gcggtactcg
     4621 ttgtaggtgg tggcgccgat gcacttcagc tgaccggtgg acagcaccgg cttaagcagg
     4681 ttcgaggcgt cgagcgtgcc gcccgaggcg gcgcccgcgc cgatcacggt gtggatctcg
     4741 tcgatgaaca ggatcgcgtt cgggttgtcg accagctgct tcagcaccgc cttcaggcgc
     4801 tgctcgaagt cgccgcggta tttcgtgccc gcgaggagcg cgcccatgtc gagaacgtat
     4861 acggtcgact tggtgaggag atcagggacg ttaccctcga cgatgcgccg ggcgagccct
     4921 tcggcgatcg cggtcttgcc gacgccggcc tcgccgacga gcagcgggtt gttcttgcgg
     4981 cggcggcaca gggtctgcac cacgcgctcg agttccctgt cgcggccgat cagcggatcg
     5041 atcttgccgg cgagcgcgag cgcgttcagg ttctgggtgt agttgtcgag cgggctgttc
     5101 gaggtctcgg cctggttttc ctgctccgcg tcctggtccg cgctcttgcc ctggtgggcc
     5161 tgcggcgtct tggtgatgcc gtgcgagatg tagttgacga cgtcgaggcg cgcgatgccc
     5221 tgctgctgca ggaagtacac cgcgtgcgag tccttctcgc cgaagatcgc gaccagcacg
     5281 ttggcgccgg tcacttcctt cttgcccgag gactgcacgt gcaggatggc gcgctggatc
     5341 acgcgctgga agccgagggt gggctgggtg tcgacctcgc ggtcggcggc gatgcgcggg
     5401 gtctgctcgg tgatgtgctg cgtcaggttc ttgcgcagct cgtccatgtt cgcgccgcag
     5461 gcgcgcagca cttcggcggc ggtcgggttg tcgagcagcg cgagcaggag gtgctcgacc
     5521 gtaatgaact catggcgctt ttgccgggcc tcgacaaacg ccatgtgcag actgacttca
     5581 agctcctgcg cgatcatgtt gctaagtttc ctccatcacg cactgcagcg gatgctgatg
     5641 cttccgagag tatgccgcga cttgctgcac tttggtggac gccacatccc gcggatagac
     5701 cccacagacg ccgatgcctt cccggtggac cttga
//
LOCUS       DATBSZ010000214        10441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2292861, whole genome shotgun sequence.
ACCESSION   DATBSZ010000214 DATBSZ010000000
VERSION     DATBSZ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2292861"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /locus_tag="VIG70_13520"
     CDS             <1..1691
                     /locus_tag="VIG70_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY8251619.1"
                     /translation="DGRKAGYGELAEAAMALPVPEKVALKDPKDFRIIGRPTTRLDAR
                     AKSSGRQDFGIDVRRPDQLTAVVARPPVFGARLSSVDDSAARAVKGVKAVLRVPLDRG
                     AEGVAVVADGYWPAKLGRDALKLQWNTTAVEKVDSTRQLAQYRELASRPGPRHFDADM
                     TPLATAPRKLEAEFVFPYLAHAAMEPLNCTVALSEGSAELWTGSQCPGLDAAAAARSL
                     GIKPEQVKVHVQMAGGGFGRRFASGSDFVVEACEVARAARAAGVNAPVRTLWSREDDM
                     RGGYYRPMHLHRARIGFDERGNVLAWDHVIVGQSITTGSVFEQFQVKNGIDATATEGM
                     RDPYAIPMRLTVHHPKVNVPVLWWRSVGSTHTAFVMETLLDEIARATKQDPVAYRMRL
                     FGDKHPRHRAALQLAVEKSDYGKKRLPAGRAWGVAVHESFDSVVAYVVEASVREGRPV
                     LRRVTAGVHCNLAVNPHSVEAQVQGAALMGLSMCLPGSAITLKEGVVEQSNFGDFTVP
                     RITDMPAFAVHIVPSADAPTGMGEPGLPPLAPAFANAVARLTGKPLRQLPFNLA"
     gene            complement(1838..2428)
                     /locus_tag="VIG70_13525"
     CDS             complement(1838..2428)
                     /locus_tag="VIG70_13525"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEY8251620.1"
                     /translation="MRWIVAALACVLTGCISTGGREAQRYYILEDAGAKAAESKAARA
                     GVLLLTPPTTSSFYDTQGIAYSRAPGMRAYYQYHSWTEAPGRRIGELLGARLERSGAF
                     SAVASVPGAVRGELVLSIHLTELYHDAAAAPGTARVALVAELVDPSRRALVTRRSFEA
                     SAPAATYDAPGAVQAFNRAIALLLDDVTAWVDGAAR"
     gene            complement(2428..3354)
                     /locus_tag="VIG70_13530"
     CDS             complement(2428..3354)
                     /locus_tag="VIG70_13530"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY8251621.1"
                     /translation="MEPEARYALVGTAVLVLVALLVGAVLWLRGTGEELDDRRYKIYF
                     ERQSLEGLQVRSDVKMKGIRVGTVNGFRISAWRPGTVEVFIRVDGTTPVRQSTQAVVE
                     RHLITGIASILLVNVSEDSPPLTQAPQGEVYPVIAEGESQIQQVSESIQQLAQRAGDT
                     MQRINAVLSPQNERALAEVLQNLQRISANAERSLAAAGGAADEVRALARELRAETARL
                     AARYDALGEESGVAVREVAASMRQLSADATRTLQRVDALLASGDVELRVTARELRATA
                     DSLGALARRFNDPRSILFGPPEGALGPGEGRR"
     gene            complement(3354..4112)
                     /locus_tag="VIG70_13535"
     CDS             complement(3354..4112)
                     /locus_tag="VIG70_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8251622.1"
                     /translation="MERVLEVDSIVTRFGPQAIHDGVSFEVARGEVVALIGGSGTGKS
                     VLLREIIGLLRPASGSIRLLGTEVWRSSPGELNALRRRFGMLFQDGALFSSLTVAENI
                     AVPFRENTDLPMDIIAPLAGFKLALVGLPPEAGKKTPAQLSGGMRKRAALARALALEP
                     EVLFLDEPTSGLDPVGAREFDRLLRLLADSLGLTVFIVTHDLDTLLSIIDRAIVLSAG
                     KVIADGPVAAVMKADDPWIRDYFSARAVLAPAPA"
     gene            complement(4113..5222)
                     /locus_tag="VIG70_13540"
     CDS             complement(4113..5222)
                     /locus_tag="VIG70_13540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8251623.1"
                     /translation="MLEHRIEDGASVLYLSGVWRLSNLGAIVDALRSLKLDAGRTFLL
                     DGSRLEALDTAAGFTLFRHLAGLGCSESMVSGRGFDQRHSRLLKLVHERMKCPPAAAR
                     SIHLGLVQRVGAAAINLWRLLVSHNAFVGAVALDALDLVPRPRRFRLRETVSQFESAG
                     LDAVPIVALVTFLIGVVFAYLLGQQALRYGANIFVVDGVGLAMCRELSPLLAAVIVAG
                     RSGAAFTAQIGTMKVQEEIDAISTLGLSPIQVLVIPRLVALTLALPLLVFVGDAAGIA
                     GGMLVAAWQLDIAPATFLERLHDVLPLRALVVGLVKAPVFAVFIALIACRMGMLVARD
                     ARSVGVNTTSTVVQSIVWVIVLDAAFAVAFQRLGI"
     gene            5421..5627
                     /locus_tag="VIG70_13545"
     CDS             5421..5627
                     /locus_tag="VIG70_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251624.1"
                     /translation="MFTSNGTLLLALLALPFAGSLAAALFPANARNREAWLAGAVALA
                     VHGLAWTGGALCALLTFFDRAAGH"
     gene            6695..7747
                     /locus_tag="VIG70_13550"
     CDS             6695..7747
                     /locus_tag="VIG70_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY8251625.1"
                     /translation="MKMVNLSRVAAISAALCIPYSAAQAAAAYKIVTADTRGTYHAIA
                     GDLAKLVAPAADIQLEPVPTSGSAENIKLLRFEPHVKLAIVQADVYQAFVDRATKANP
                     EASRIIRPLRVILPLYNTEIHYIARADSPLTYLHDLKDAKINGGLVGSGAAFITHTLY
                     RMMFKDAIPDANASYLPNAEALVKLISDKSVDVAVVAAGQPAPIIANMKPEAQKYIKL
                     LKFDPAHPTSKQPLAIYSPATVRAKSYPNILSEDFTTVSVGAFLVTYDYNLQGTVEHL
                     TRFARSLCRNFSVLQEKGHPKWREVQLALPALNDGWTYYGPTAKEIRSCLAAAQKPAA
                     PAKACAAEEKILGLCG"
     gene            complement(7768..10077)
                     /locus_tag="VIG70_13555"
     CDS             complement(7768..10077)
                     /locus_tag="VIG70_13555"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8251626.1"
                     /translation="MKRTQPLGPAVRILLLEDDPISVEIVGTYLRRITFAEVEVHVAA
                     ALGDALALLAQADVELVVADLYLPDSSGAATVDSLVQVTGCPVIAITADSDPGLRDAT
                     LACGAFEFLQKDDLTEATLMRLVRLATMQARTYRSLRESREAEAALRESEGRFRSLAG
                     FGQSAVVKSEPAELVEKAARAIFDALGPDAVAYIDIDPSTGKLMLRALVAPALPDVRP
                     APVRSQGNDPVLRVFASGQVALAEGLELPFPWARDLGRAALVPVRTDEKVRGVLCICS
                     KQTAAFSAEEINFLQATSSVLSTALQRVESEARLAYLAQFDPLTGLPNRALLADRFSQ
                     MIVQARRRDSPLAVLFIDLDQFKDVNDTLGHAGGDGLLKQVGARLESAVRTADTVARI
                     SGDEFAIVLTDLVRPEDAALVAQKIIDDLGMPVDVDGREVFVTASVGIATYPADGIEA
                     ETLIGAADAAMYRAKQLGRNTYQFFTAEINQRSRARVQLGSELRRALEREEFTLSYQP
                     RYRLASRQPSGAEALLRWEHPERGMVPPHEFIPVLEETGLIVPVGEWAIRRACKDLRQ
                     WQSVASAIGPVSVNLSARQFRQPDLHERIKVLVSSSGVDPRLIEFEITESQLMQDPPH
                     AIQVMRALCDAGMSIAIDDFGTGYSSLAYLTRFPVSALKIDRSFVRDMARDKNDATIV
                     RTIIEMGHSLGFTVIAEGVETEEQATLLHLLRCEQAQGYLFGRPMPAHELIRLYDAVR
                     GVQVLSPSLRRKRKTASASLQTPPATASS"
     gene            complement(10192..>10441)
                     /locus_tag="VIG70_13560"
     CDS             complement(10192..>10441)
                     /locus_tag="VIG70_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8251627.1"
                     /translation="NLQTTVDYLARFARSLCQNFATLQAKGHPKWREVDLTLPALNKG
                     WTYYPPTSREIRNCLADRSKAGRAKACSAEERILGFCN"
BASE COUNT         1756 a         3617 c         3465 g         1603 t
ORIGIN      
        1 cagatggtcg caaggcgggt tacggcgagc tagccgaggc cgccatggcg ctgcccgtgc
       61 ccgagaaggt cgcactgaag gatcccaagg atttccgcat catcgggcgc cccaccacgc
      121 gcctcgacgc gcgtgccaag agcagcggac gccaggactt cggcatcgat gtccgccggc
      181 ccgatcagct gacagccgtg gtggcccgcc cgccggtgtt cggcgctcgc ctgtcctcgg
      241 tggacgacag cgccgcgcgc gccgtcaagg gcgtgaaggc cgtgctgcgc gtgccgctgg
      301 accgtggcgc cgagggcgtg gcagtggttg ctgacggcta ttggccggcc aagctcgggc
      361 gcgatgcgct gaagctgcag tggaacacga ccgccgtcga gaaggtggac agcacgcggc
      421 aactggccca gtaccgcgaa ctggccagtc gccctggccc gcgccacttc gacgccgaca
      481 tgaccccgct cgccacggcg cctcggaaac tggaagctga gttcgtgttt ccctacctgg
      541 cccacgcggc tatggagccg ttgaactgca ccgtcgcgct ttccgaggga agcgccgagc
      601 tgtggaccgg ctcgcagtgt cccggtctgg acgccgctgc cgccgcgcgc tcgctcggga
      661 tcaaacccga gcaggttaag gtgcatgtgc agatggccgg cggcggcttt ggccggcggt
      721 ttgcgagcgg cagtgacttc gtggtcgagg cctgcgaagt cgccagggcc gcccgtgccg
      781 ccggcgtgaa cgcgccggtg cgcacgctct ggagccgcga ggacgacatg cgtggcggct
      841 actaccgccc gatgcacttg caccgcgcac gcatcggctt cgatgaacgc ggcaatgtgc
      901 tggcctggga ccatgtcatc gtcggccagt ccatcaccac gggctcggtg ttcgagcagt
      961 tccaggtgaa gaacgggatc gatgccaccg ccacggaggg catgcgcgac ccctacgcga
     1021 ttccgatgcg tctgaccgtg caccacccaa aggtcaacgt accggtgctg tggtggcgca
     1081 gcgtgggttc tacccacacc gccttcgtca tggaaacctt gctcgacgaa atcgcccgcg
     1141 ccaccaagca ggacccggtg gcctaccgga tgcggctgtt cggcgataag cacccgcgcc
     1201 accgcgccgc actgcagcta gcggtggaaa agagtgacta cggcaagaag cgcctgcctg
     1261 ccggccgcgc ctggggtgtt gccgttcacg agtccttcga ctcggttgtt gcctacgtgg
     1321 tggaggcctc ggtgcgagag ggtcgcccgg tgctgcgccg cgtgaccgcc ggcgtgcact
     1381 gcaacctcgc ggtcaatcca cacagtgtgg aagcacaggt gcagggcgcg gccctgatgg
     1441 gcctgtcaat gtgcctgccg ggttccgcca tcacgctcaa ggaaggcgtc gtcgagcaga
     1501 gcaactttgg cgacttcacc gtgccgcgca ttaccgacat gccggcattc gcggtccaca
     1561 tcgtccccag cgccgacgcg cccaccggca tgggcgaacc cggcctgcca ccgttggccc
     1621 ctgcgttcgc caacgccgtc gcgcggctga cgggcaagcc cctgcgccag ctgcccttca
     1681 atttggcgtg acccggcttg gggtacggct gggggtatta gcgcgtggac ttgtacagat
     1741 cttcgtccac taccgtctca ggaaggccac ctacgtgcgg tgtccggttg acgccgcctc
     1801 caccagttca cggccgcctg gagcggccgt taccttttta gcgcgcggcg ccgtcgaccc
     1861 aggcagtgac gtcatcgagc agcaaggcga tcgcccggtt gaacgcctgc accgcccccg
     1921 gcgcatcgta ggtcgcggcc ggcgccgagg cctcgaagct gcgccgcgtc accagcgcgc
     1981 gccgcgacgg atcgaccagc tcggcgacga gcgcgacgcg cgcggttccc ggcgcggcgg
     2041 ccgcatcgtg atagagctcg gtcaggtgaa tgctcaggac cagctcgccg cgcaccgcgc
     2101 ccggcacgct cgccaccgca ctgaacgcac cgctgcgctc gaggcgcgcg ccgagcagct
     2161 cgccgattcg gcggcccggg gcctcggtcc agctgtggta ctggtagtag gcgcgcattc
     2221 cgggggcccg gctgtaggcg atgccctgcg tatcgtaaaa gctcgatgtg gtcggcggcg
     2281 tgagcagcag taccccggcc cgcgccgcct tcgattcggc ggccttcgcg cccgcgtctt
     2341 cgaggatgta gtagcgctgc gcttcgcgcc cgccggtgga aatgcagccg gtgaggacac
     2401 aagcgagcgc cgcgactatc catcgcatca cctgcggccc tcgccgggcc cgagcgcgcc
     2461 ttcgggcggc ccgaacagga tgctgcgcgg atcgttgaag cggcgcgcga gcgcgccgag
     2521 cgagtccgcg gtggcgcgca gttcgcgcgc cgtcacgcgc agctcgacgt cgccgctcgc
     2581 gagcagcgca tccacgcgct gcagggtgcg cgtcgcatcg gcgctcagct ggcgcatcga
     2641 ggcggcgacc tcgcgcaccg cgacgcccga ttcctccccg agcgcgtcgt agcgcgcggc
     2701 gagcctggcg gtctccgctc tcagctcgcg cgccagggcc cggacttcat cggccgcgcc
     2761 gcccgccgcg gcgaggctgc gctcggcatt cgccgagatg cgctgcaggt tctgcaggac
     2821 ctcggcgagc gcgcgctcgt tttgcggcga gagcaccgcg ttgatgcgct gcatggtgtc
     2881 gcccgcgcgc tgcgcgagct gctggatcga ctcggagacc tgctggatct gcgactcgcc
     2941 ttcggcgatc acggggtaga cctcgccctg cggcgcctgc gtcagcggcg ggctgtcctc
     3001 gctgacgttg acgagcagga tgctcgcaat tccggtgatc aaatggcgct ccaccaccgc
     3061 ctgcgtgctc tggcgcaccg gcgtcgtgcc gtcgacccgg atgaacactt ccaccgtgcc
     3121 gggacgccac gccgagatgc ggaagccgtt caccgtgccg acccggatgc ctttcatctt
     3181 gacgtcgctg cgcacctgca gcccttcgag cgactggcgc tcgaaataga tcttgtagcg
     3241 gcgatcgtcg agctcctcgc cggtgccgcg cagccacagc accgcgccga ccagcagcgc
     3301 cacgagaacc agcaccgcgg tgccgacgag cgcgtagcgg gcttcgggtt ccatcaggcg
     3361 ggcgctggcg ccagcactgc tcgcgcagag aagtaatccc ggatccaggg atcatcggcc
     3421 ttcatcaccg ccgccaccgg cccgtccgca atcaccttgc ccgccgacag cacgatcgcg
     3481 cggtcgatga tcgagagcag cgtgtcgagg tcgtgcgtga cgatgaatac cgtgaggccg
     3541 aggctgtcgg cgagcagccg cagcaggcgg tcgaattcgc gcgcgccgac cggatcgagc
     3601 cccgaggtgg gttcgtcgag aaacaggact tcgggctcga gcgcgagcgc gcgcgccagc
     3661 gccgcacgct tgcgcatgcc gcccgagagc tgcgccgggg ttttcttgcc ggcctctggc
     3721 ggcaagccga cgagggcgag cttgaatccg gcgagcgggg cgatgatgtc catcggcagg
     3781 tcggtgttct cccggaaggg aacggcgata ttctcggcga ccgtcagcga gctgaacagc
     3841 gccccatcct ggaacagcat gccgaagcgg cggcgcagcg cgttcagctc gccggggctc
     3901 gagcgccaga cctcggtgcc gaggaggcgg atgctgcccg acgcgggccg caacaggccg
     3961 atgatctccc tcaggagcac cgacttgccg gtgccgctgc cgccgatgag cgccaccacc
     4021 tcgccgcgcg ccacctcgaa gctgacgccg tcgtggatcg cctgcgggcc gaagcgcgtg
     4081 acgatcgagt cgacctcgag cacgcgctcc actcagatcc ccaggcgctg gaacgccacc
     4141 gcgaacgcgg cgtcgagaac gatgacccag acgatgctct gcaccaccgt ggaagtggtg
     4201 ttcacgccca cgctgcgcgc gtcgcgcgcc accagcatgc ccatgcggca ggcgatcagc
     4261 gcgatgaaaa cggcgaacac cggtgccttg accaggccca cgaccagcgc cctgagcggc
     4321 agcacgtcgt gcaggcgctc gaggaaggtc gcgggcgcga tgtcgagctg ccaggcggcg
     4381 accagcatgc cgccggcgat tcccgctgcg tcgccgacga acacgagcag cggcagcgca
     4441 agcgtgagcg cgacgaggcg cgggatcacc agcacctgga tcggcgacag gccgagcgtg
     4501 ctgatggcat cgatctcctc ctgcaccttc atggtgccga tctgggcggt gaacgcggcg
     4561 ccggagcggc cggcgacgat caccgccgcg agcagcggcg aaagctcgcg gcacatggcg
     4621 aggccgaccc cgtcgaccac gaagatattc gcgccgtagc gcagcgcctg ctgcccgagg
     4681 agataggcga ataccacgcc gatgaggaac gtcaccaggg cgacaatcgg gaccgcgtcg
     4741 aggcccgcgg actcgaactg cgacacggtt tcccgcaagc ggaagcgccg gggcctgggg
     4801 acaaggtcga gcgcatcgag ggcaacggcg ccgacgaagg cgttgtgcga taccagcagc
     4861 cgccacaggt tgatcgccgc cgcgccgacg cgttgcacca gcccgaggtg aatcgagcgc
     4921 gccgccgccg gcgggcactt catgcgctcg tgcaccagct tgagcagccg cgagtgacgc
     4981 tggtcgaagc cgcgcccgct caccatcgat tcgctgcaac cgagccccgc gaggtgccgg
     5041 aacagcgtga agcccgcggc ggtgtcgagc gcctcgagcc tgctgccgtc gagcaagaag
     5101 gtcctgcccg cgtccagctt cagcgagcgc aaggcgtcca cgatcgcgcc caggttggac
     5161 agccgccaga ccccgctcag gtaaagaacg ctcgcgccgt cctcgatcct gtgctctagc
     5221 acttcatctc ccgcggcgct tgtccggctc catgaggtgc tgctcctcct cgctctgacc
     5281 gaacggcgga gcggcagcgg aaaggaaagc agccaggagc agccagcggc tcatctcgcc
     5341 ttcgacttca cctcgttgta ccagacccgt ggcgctcgag tgacttcatc atagcggctt
     5401 caggcttcca gtaagctcaa atgttcacgt ccaacggaac gctgctgctc gccctgctcg
     5461 ccttgccatt cgccggcagc ctggcggctg cgctgttccc cgccaatgcc cgaaaccgcg
     5521 aagcatggct cgccggggcg gtcgcgctgg cggtgcacgg gctggcgtgg accggtggtg
     5581 ctctatgcgc gctactgaca ttctttgacc gtgctgccgg gcattaggaa gaactaacca
     5641 ttgccccggc gccggcagcg aacaacaatc cacaggggac cggcaacgta atgcaaaacg
     5701 aaacagtgca gggcatcgcg cccgccgccg cacgaggcgc tatcgcgcac gacgtgcgcc
     5761 tgtgcgtggc ttgtctgcat gaaaatcgcg gcggcgagaa gtcttgcgaa gcctgcggca
     5821 cgtctttgtt tctcatcctc tgcggtgcgt gcgaagccgc caacagcgca ggggatgtgc
     5881 gctgccatgg ctgcggcgcc gcactcggca ccgagtccag cgacaagccc gcggagcccg
     5941 cggcggcggc gcctccggcc agcgaagcga gccttgggcc caccaaggcg agcgacaagc
     6001 ccgaggcgga gcctccagcc gcccgaataa gttctgcgcg caccgaggtc agcgaggagc
     6061 ccgagacggc gcctccagtc gggcgaatga gccttgtgcg caccgaagcg agggacgagc
     6121 aagcggaagc gcctccggcc agacgagcca accgttggcg ctccgccctg attctggcgg
     6181 gagtcgtggt tacgacggcc ggcgtcacgc accacttttc ctcgcgaacc gcggaccgcg
     6241 ctccccaagg caaggtcatc gaagcggcgg ccgtgaagtc ggtcgaaccg ccaccgagag
     6301 cggagcccaa agcaggcggc ccgaatgtca cgcataccca gcccgcggat gccagccaga
     6361 cggcgccgtc tgcgccagtg acgcgagcgg ctgtcatcga agtggcgccc gtgaagtcgg
     6421 tcgagccgcc ccgtcgcgcg ccgccaccga gaacggagcc cagggcggtc acccctgccg
     6481 gcggtccgtc ggcgagcgtt gggtttacgc cccaagccgg ctcggcctat ccgcctgtaa
     6541 cgcatacgca gcaagcggca gcggcacaag tcgtgccggc cgccgttgtg ccggccgccg
     6601 ttgtgccggc caccactcag aagtccgatt ctggttgtgg cgaagcggtc gccgccctcg
     6661 gtctctgcaa cgccagggca caaagcggag gtaaatgaaa atggtcaact tgtctcgcgt
     6721 agcggcaata tcggctgcgc tgtgtattcc gtacagcgcc gcgcaagcag cggccgctta
     6781 caagatcgtc accgccgaca cgcgcgggac gtatcacgcc attgccgggg atctcgcaaa
     6841 gctcgtcgca cccgccgcgg acattcaact ggaaccggtg ccgacctcgg gatcggccga
     6901 aaacatcaag ctgcttcgct tcgagccgca cgtgaagctg gccatcgtcc aggctgacgt
     6961 gtatcaggcc ttcgtggatc gcgccaccaa ggcgaacccg gaagcgtcca ggatcatccg
     7021 gcccctgcgc gtcatcctgc cgctctacaa caccgagatc cactacatcg cgcgggccga
     7081 ctcgccactc acctatctgc acgacctcaa ggacgcgaag atcaacggcg gcctggtggg
     7141 aagcggcgcc gccttcatca cccacacgct ctaccggatg atgttcaagg acgccatccc
     7201 ggacgcgaac gcgagctacc tgccgaatgc cgaagcgctc gtcaagctca tttccgacaa
     7261 gtcggtggac gtggcggtcg tcgccgccgg tcaacccgcg ccgatcatcg ccaacatgaa
     7321 gccggaggcg cagaaataca tcaagctgct gaagttcgat ccggcgcatc cgacgagcaa
     7381 gcagccgctc gccatctact cgcctgcgac ggtgcgcgcg aagagctacc cgaatatcct
     7441 gagcgaggac ttcaccaccg tttcagtcgg cgcttttctg gtgacctatg actacaactt
     7501 gcaaggcacc gtggagcatc tgacgcggtt tgcgcgctcg ttgtgccgga acttctcggt
     7561 cctgcaggag aaaggtcatc ccaagtggcg cgaagtgcag cttgcgttgc cggcgctcaa
     7621 cgacggatgg acctactacg ggccgacggc gaaggagatc cgtagctgcc tggcggcggc
     7681 acagaagccg gcggcaccgg ccaaggcgtg cgcggcggag gaaaagatcc tcggtctttg
     7741 cggctagggt cggggcggcc caggccccta gctgctcgcc gtcgccggtg gcgtttgcag
     7801 gcttgcgctc gccgttttcc ttttcctgcg cagggaaggg ctgagcacct ggacgccgcg
     7861 tactgcatcg tataaacgga tcagctcgtg tgccggcatc ggccggccga acaggtagcc
     7921 ctgcgcctgc tcgcaacgca gcaggtgcaa cagcgttgcc tgctcctcgg tttcaacccc
     7981 ttccgcgatg accgtgaagc ccaggctgtg ccccatctcg atgatcgtgc gcacgatggt
     8041 cgcgtcgttc ttgtcgcgcg ccatgtcccg gacgaacgag cgatcgatct tcagggcgct
     8101 gacgggaaat cgcgtcagat acgcaaggct cgaatagccg gtgccgaagt catcgatggc
     8161 gatgctcatc ccggcgtcgc acaacgcgcg catcacctgg atggcgtgcg gcggatcctg
     8221 catcagctgg ctctcggtaa tctcgaattc aatcagcctc ggatcgacgc cgctggagga
     8281 caccagcact ttgatgcgct cgtgcagatc gggctggcgg aactggcgcg cggacagatt
     8341 gaccgagacc ggcccgatcg ctgaagccac cgactgccat tgcctcaggt ccttgcaggc
     8401 gcggcggatc gcccattcgc cgaccgggac gatgagaccg gtctcctcga gaaccgggat
     8461 gaactcgtgc ggcggcacca tgccgcgttc agggtgctcc cagcgcagga gcgcctcggc
     8521 gccgctcggc tggcggctgg cgagccggta cctcggctga taggaaagcg tgaactcttc
     8581 gcgctccagg gcccgccgga gctccgatcc gagctgcacc cgggcgcgcg agcgctggtt
     8641 gatctccgcg gtgaaaaact gatacgtgtt gcggcccaat tgcttggccc ggtacatcgc
     8701 cgcatcggca gcgccgatca gggtctcggc ttcgatgccg tctgccgggt acgtagcgat
     8761 ccctacgctc gccgtgacga atacctccct cccgtccacg tcgaccggca tgcccaggtc
     8821 atcgatgatt ttttgcgcga ccagcgccgc gtcctcgggg cgcaccaggt ccgtcagcac
     8881 gatcgcgaac tcgtctccgc tgatgcgcgc cacggtatcg gcggtacgca cggcggactc
     8941 cagccgcgcc cccacctgct tgagcaaacc atcgccgccc gcatggccca gggtgtcatt
     9001 cacgtccttg aactggtcga ggtcgatgaa cagcaccgcg agcggcgagt cccggcgcct
     9061 cgcctgcacg atcatctgcg agaaacggtc ggcaagcagc gcccggttgg gcaggccagt
     9121 gagcgggtcg aactgcgcga gataggcgag ccttgcctcg ctctcgacgc gctgcagcgc
     9181 cgtcgacagc acgctcgacg tcgcctggag aaaattgatc tcctcggcgc tgaacgccgc
     9241 cgtctgcttg ctgcagatgc acagcacgcc gcgcactttc tcgtcggtgc gcacgggcac
     9301 cagcgctgcc ctgcccagat cgcgcgccca tggaaacggc aactccaggc cctcggcaag
     9361 cgccacctgg cccgatgcaa acactcgcag gacggggtcg tttccctgcg agcgaaccgg
     9421 cgcagggcga acgtcgggca gcgcgggagc gacgagcgca cgcagcatca gcttgccggt
     9481 gctcgggtcg atgtcgatgt aggcaaccgc gtcggggccc aaggcatcga agatggcgcg
     9541 cgcggccttt tccacgagct cggccggctc ggatttcact accgccgact ggccgaagcc
     9601 cgcaaggctg cggaagcgcc cttcgctctc ccgcaacgct gcttccgcct ccctgctctc
     9661 gcgcagcgag cggtaggtac gcgcctgcat cgtcgcgagc cgcacaagcc gcatgagcgt
     9721 cgcttccgtc agatcgtcct tttgaagaaa ctcgaaggcg ccgcaggcaa gggtggcatc
     9781 gcgcagcccc gggtcggaat ccgcggtgat cgcaatcacg ggacagcccg tcacctggac
     9841 caggctatcg accgtagcgg cgcccgagga gtccggcagg tagagatccg cgacgaccag
     9901 ctcgacgtcg gcttgcgcaa gaagggcgag cgcgtcgccc agcgccgccg cgacatgcac
     9961 ctcaacctcg gcgaatgtaa tgcgccgaag atacgtaccg acgatctcta cgctgatcgg
    10021 gtcgtcttcg agcaacagga tccgcacggc cggcccaagc ggctgtgtgc gcttcaagaa
    10081 atcccccctc ccctgactag atgctgccgt taccgatccg gattctggca cgccgcgggg
    10141 agcaacggtg agtgcaccgc agcagtcagc acgccacttt gggataaacg ttcagttgca
    10201 gaacccgagg atgcgctcct cggcggagca tgccttggcc cggccggctt tcgatcgatc
    10261 ggcgaggcag ttgcgtatct ctctgcttgt cggcgggtag taggtccagc ccttgttgag
    10321 ggcaggcaga gtgaggtcga cctcccgcca tttcggatgg cccttcgcct gcagcgtcgc
    10381 gaagttctgg cacagcgagc gggcgaatcg cgcgagatag tcgacggtcg tctgcaggtt
    10441 g
//
LOCUS       DATBSZ010000215         4103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2019480, whole genome shotgun sequence.
ACCESSION   DATBSZ010000215 DATBSZ010000000
VERSION     DATBSZ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2019480"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..551)
                     /locus_tag="VIG70_13565"
     CDS             complement(177..551)
                     /locus_tag="VIG70_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8251628.1"
                     /translation="MFDHVKFGVSDYAASKAFFLKALEPLGVAVVSEGPPTYGVELSP
                     KGKASLCLYQTEEKPAHLHLAFTAESRQQVEAFYRAALEAGGKDNGAPGLRPHYHANY
                     YAAFVIGPDGHNIEVVCHVPEA"
     gene            complement(647..1456)
                     /locus_tag="VIG70_13570"
     CDS             complement(647..1456)
                     /locus_tag="VIG70_13570"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8251629.1"
                     /translation="MHPPAEIYTQRANPAFEAELAHRSAARDAAFLLPWLRPGMRLLD
                     VGCGPGAITLGLAEAVAPGQVVGIDIQPAQVEQARSLASTRGAATVSFEVGSIYALAF
                     PERSFDAVFANGVVMHLREPVRALAELRRVLRPGGIAGVRDPDFGATLYAPLTPVFER
                     LLSVRARVRRHNGGDPFLGRHYRRLLLEAGFERAEAGASVDSAGSAERTRRHAGFLKA
                     MLHGFTPTAVSQGWLEQSAIGTIVSEIDAWAQRPDAFYAATWCEAIGWASD"
     gene            complement(1463..2443)
                     /locus_tag="VIG70_13575"
     CDS             complement(1463..2443)
                     /locus_tag="VIG70_13575"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8251630.1"
                     /translation="MDLASLRLITAALLAAAPIAAQSTEAPAYPTKPIRLLVSGAPGT
                     PPDALARIVTEPLGAALGKPVLVENRPGGSGALALGVVARSAPDGYTLATIGLPQIVA
                     PSLVHEISYDTIRDLAPVIQLTWSANVLVVRASSPVKTVAGLVELARARPGALTYASG
                     GNGTPSHLALELLKHHAGIDIRHIPYKGIASGLAAIMSEQVDVAFAGAATALPLINGG
                     RLRALATAGAQRLPALPELPTIAELGFVGYQQNEWYGVVAPAGTPPQVIAKLAAELAR
                     IVASPQTRSRLANLGLYPVENSGPGELGALIRAELPRWRKIVREAGIRAD"
     gene            2522..3463
                     /locus_tag="VIG70_13580"
     CDS             2522..3463
                     /locus_tag="VIG70_13580"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8251631.1"
                     /translation="MRLTQVRDFLAVVECGGIRAAARRLGVSQPTITKSIRSLEAELH
                     VQLLARNARGIVPTASGRAFFSRARVAHSELRKAEEEAAEVGGSSAGSVTFGVGPTFA
                     ALIVPDAVARFREQFPRARVRIVEGLARHLLRSVQDETLDFAMGLRTIAELDPSLRFR
                     PLYRSELVVVVRKGHPLRHARALADLAGADWLSTSTLDLPGGPVERLFASAGLTPPRL
                     AVQCESQSTMVALLSRTDMLGIVQRRTLVAAPGCELLQEVPVAETMPSVTAGIYTRAD
                     TPLTRVAAAMAKAATTVARGLAGPHRYRLVGAGRAGR"
     gene            complement(3888..>4103)
                     /locus_tag="VIG70_13585"
     CDS             complement(3888..>4103)
                     /locus_tag="VIG70_13585"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY8251632.1"
                     /translation="KLTERKLSLTVQLSAPEEYTGGELIIYPEFVAPKDQGTMTVFPS
                     FMCHNVNPVKSGVRYSLVSWLAGPPFK"
BASE COUNT          662 a         1395 c         1367 g          679 t
ORIGIN      
        1 ccgttctgcg ccttgttctg gtaaccaagc acgtgcacaa cctgcttgct gtcgagggcc
       61 acctccgagg catacagaac ggtatccgaa aagcgaacgg gcactccagg agagacagaa
      121 caacacatga tcttcaccag tctcgtgttt cttctgcact tcggggcagc gaagggtcag
      181 gcctcgggta cgtggcaaac cacttcgatg ttgtgcccgt ccggaccaat gacgaaagct
      241 gcatagtagt tcgcgtggta gtgcgggcgc agacccggcg caccattgtc tttgccaccc
      301 gcctccagag ccgcgcgata gaaagcttcg acttgctgcc gactctcggc cgtgaacgcc
      361 aagtgaagat gcgccggctt ttcctcggtt tggtacaggc acaatgaagc cttacccttt
      421 gggctgagct cgacaccgta cgtcggcggc ccttccgaga caacagctac gccgagcggt
      481 tcgagtgcct tgaggaagaa cgctttgctc gctgcatagt cgctgactcc gaatttgacg
      541 tggtcaaaca tgagttcgcc tgatgtcact caagctcgaa tctcccatgt ctgatcgcat
      601 cctgtctatg gtgcccgccg cgcatcggcc gaacgcggtt cgagcttcag tcgctggccc
      661 acccgatcgc ttcgcaccac gtcgccgcgt agaacgcgtc cgggcgctgc gcccaggcgt
      721 cgatctccga cacaatcgtg ccgatcgccg actgctccag ccacccttgc gagactgccg
      781 tcggggtgaa gccgtggagc atcgccttga ggaagccggc atgccgacgc gtcctctccg
      841 ccgagcccgc gctgtcgacc gacgcgcccg cctccgcgcg ctcgaaaccc gcctcgagca
      901 gcaaccgccg atagtggcgg ccgaggaaag gatcgccacc gttgtgacgc ctgacccgtg
      961 cccgcaccga gagcaagcgc tcgaacaccg gcgtcaaggg agcgtagagc gtcgcgccga
     1021 agtccggatc gcgcacgccc gcgatgccgc ccgggcgcag cacgcggcgc agctcggcga
     1081 gcgcccgtac cggctcgcgc agatgcatca cgacgccgtt ggcaaagacc gcgtcgaacg
     1141 agcgctccgg gaacgcaagc gcatagatgc tgccgacctc gaagctcacc gtagcagccc
     1201 cgcgcgtgga cgcgagcgag cgcgcctgct cgacctgcgc gggctggatg tcgatgccga
     1261 ccacctggcc gggagccacg gcttcggcaa ggcccagcgt gatggcgccg gggccgcagc
     1321 ccacgtcgag cagccgcatg ccgggccgca accacggcag gaggaacgcc gcgtctcggg
     1381 cggccgagcg atgcgccagc tcggcttcga acgccgggtt ggcccgttgg gtatagatct
     1441 ccgccggagg atgcacgccg cctcagtccg cgcggatacc cgcttcgcgc actattttcc
     1501 gccaacgcgg aagctcggcg cggatgagcg cgcccagctc tccggggccg gaattctcga
     1561 ccggatacag gcccaggttc gcgaggcgcg accgggtctg cggcgaggcg acgatccgcg
     1621 ccagctcggc agcgagcttg gcgatgacct gcggaggcgt cccggccggc gcgacgaccc
     1681 cgtaccactc gttctgctgg tagccgacga agccgagctc ggcgatcgtc ggcagctcgg
     1741 gaagcgccgg cagacgctgc gccccggcgg tggccagcgc gcgcagcctt cctccgttga
     1801 tgagcggcag cgccgtggcg gcgcctgcga aagcgacatc gacttgctcg ctcatgatcg
     1861 cggcgaggcc cgaggcgatc cccttgtagg ggatgtgccg tatgtcgatg cccgcgtggt
     1921 gcttgagcag ctccagcgcg aggtgcgaag gcgtgccgtt gccgccggac gcgtacgtga
     1981 gcgcgcccgg cctcgctctt gcgagctcga cgagaccggc aaccgtcttc accggggacg
     2041 aggcgcgcac gacgagaacg ttcgcgctcc acgtgagctg gatcaccgga gcgaggtcgc
     2101 ggatcgtgtc gtaggagatt tcgtgcacca ggctgggcgc gacgatctgc ggcaggccga
     2161 tcgtcgcgag cgtgtaaccg tcgggcgcgg acctggcgac gacgccgagc gcgagtgcgc
     2221 cgctgccgcc gggtcggttc tccaccagta ccggcttgcc tagcgcggcg cccaggggct
     2281 cggtgacgat gcgcgcgagc gcgtcgggcg gtgttccggg cgcccccgac accagcagcc
     2341 ggatcggctt ggtggggtag gcgggcgctt cggtcgattg cgcggcaatc ggcgccgcgg
     2401 cgagcagcgc cgcggtgatc aggcggagtg aggcgaggtc catggcgtga agtagaccag
     2461 accggccgtc ggcggcataa gatggctgcc ggttgaccct gatagcccgg aggctatcgc
     2521 cgtgagactg acccaggtgc gagactttct ggccgtggtc gaatgcggcg gcatccgcgc
     2581 cgcggcacgc aggctcggcg tgtcccagcc gaccatcacc aagagcatcc gcagcctcga
     2641 agccgagctg catgtgcagc ttctcgcgcg caacgcacga ggtatcgtgc cgacggcgtc
     2701 cgggcgcgcc tttttctctc gcgcccgagt cgctcactcc gagctgcgca aggccgagga
     2761 agaggctgcg gaagtcggcg gctcgagcgc cggatcggtg accttcggcg tcggcccgac
     2821 gttcgccgcc cttatcgtcc ccgacgcggt cgcgcgcttc cgcgagcagt ttccccgcgc
     2881 ccgcgtccgc atcgtcgagg gcctagcccg gcatctgctg cgctcggtgc aggacgagac
     2941 gctcgacttc gcgatgggcc tgcgcacgat cgccgagctc gatccttcgc tgcggttccg
     3001 tccgctgtat cgcagcgagc tcgtcgtcgt cgtccgcaag ggtcacccgc tgcgccacgc
     3061 gcgcgcgctc gccgatctgg caggggcaga ctggttgagc acctcgacgc tcgacctgcc
     3121 gggcggaccg gtcgagcgct tgttcgcgtc cgccggcctg acgccgccgc ggctggcggt
     3181 ccagtgcgag tcgcagagca cgatggtcgc gctgctctcc cggaccgaca tgctcgggat
     3241 cgttcagcgc cgtaccctgg tggcggcgcc gggatgcgag ctgctgcagg aagttccagt
     3301 cgccgaaacc atgccgtcgg tgaccgccgg catctatacg cgcgccgata ctccgctgac
     3361 ccgcgtcgcc gcggcgatgg ccaaggccgc gaccacggtg gcgcgcggcc tcgccggccc
     3421 gcaccggtac aggctcgtag gcgcgggccg agccgggcgg tgagccgcag gtccttgccg
     3481 acaacaacgc gctgttaggc tttactcgcc tgccatccgg ccgatgacct tgccggtctg
     3541 gttgattatg aagatcgcgt gtcctccggg atatggaagg ggtttcccct gaggcgagtt
     3601 gcgctttgtg aactcgacac gcacgtgcca attgccttcc tgcgccttcg catcgaacgt
     3661 gcacggtgtt gtggtcgtgc accggtcctt tacctgacgt ttggctgcgt cgatggcacc
     3721 cgccctatcc aacgcagacg cggacaagac ccatgaggct aagaccgccg tcatggtgag
     3781 aatgagcgcg cggcgagcga ccatgaagcc cgtcgggcgt cgcctcgacc gaggggttag
     3841 gcctcggcat tcggcttgcc cgcctcgggg ctcaaccttg tgtgaactca tttgaatggc
     3901 ggtcctgcca gccaacttac cagcgagtaa cgaacgccgc tcttcaccgg attgacgttg
     3961 tgacacatga acgacgggaa cactgtcatc gttccctgat ccttgggagc cacaaactcc
     4021 ggataaatga tcagttctcc accggtgtat tcttcaggag ccgacaattg aacggtcaag
     4081 ctcaacttac gttccgtcag ctt
//
LOCUS       DATBSZ010000216         9433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2430588, whole genome shotgun sequence.
ACCESSION   DATBSZ010000216 DATBSZ010000000
VERSION     DATBSZ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2430588"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VIG70_13590"
     CDS             <1..1114
                     /locus_tag="VIG70_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY8251633.1"
                     /translation="AGTMCPSYRATMDERHLTRGRANTLRLALSNQLGDQGDEAVKEA
                     LDLCVSCKGCKRECPTGVDMARMKIEFLARYKATHGLSARDRAIGYLPRYAPWAARLA
                     PLSNLVSSLSKSFLGFATKRELPKWRGDFFTEQGRSDGDVVLFVDTFNRYFEPENARA
                     ALKVLEAAGHRVHAPRGLCCGRTFLSVGLADEAKREARKMLDALSPYAAKGIPIVGLE
                     PSCLFSLRDEFSVLVKNGNALASSSVLLEEFLARNPGKLKFREMKADVLLHGHCHQKA
                     FDAMPAVEKVLGMIPGLKASTVQTSCCGMAGSFGYEAEHYDVSMKMAEAALLPAIRKS
                     PNALLVADGTSCRHQIADGAGREAVHVARVLADCLA"
     gene            complement(1106..2047)
                     /locus_tag="VIG70_13595"
     CDS             complement(1106..2047)
                     /locus_tag="VIG70_13595"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY8251634.1"
                     /translation="MESTLDPVLGAVLPVFGLVACGYLAGRFHIVTQASSLALNQFVY
                     AFALPAMLFVAVYRGSLEEILSGFFLLAVIGATLATAVAGFFLSHAAGSRPAESTMRA
                     LNASFANTGYLGIPLVSVAYGERAALPAALATVATNIVSFALAIVCLELFVNPRRGGV
                     RRALGGVARSPLIWPIALAFVLVALEVKIPLPAERFASLLAAAAGPCALFAIGLFVSQ
                     LSIRAGAASSWQTSVLKLVLHPLLMAAAAFWILPVDPFWAKIAVVCAALPLGATAFVL
                     AQRYKLLEAETSTGAVISTAASVVTVSLVMAFFARLG"
     gene            complement(2032..2802)
                     /gene="lgt"
                     /locus_tag="VIG70_13600"
     CDS             complement(2032..2802)
                     /gene="lgt"
                     /locus_tag="VIG70_13600"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY8251635.1"
                     /translation="MLIHPNIDPIAFSIGPLAVRWYGLMYLAGFAAGWWLGLRRIAKG
                     EAPITRAQLDDLLFLIVLGVILGGRLGYVLFYKPGHYFSHPLEIFYIWQGGMSFHGGL
                     LGVMLAMVFAARRHGVDWLRLMDFLAPLCPLGFAAGRLGNFINAELPGRVTDLPWGMV
                     FPGAGDAPRHPSQLYQFALEGVVLFAFLWWFSSKPRARGQVSAMFLLGYGVLRFVAEF
                     GREPDAFIGYLALGLTMGQWLCLAMIAGGAALLAWSRR"
     gene            2821..4500
                     /gene="ilvD"
                     /locus_tag="VIG70_13605"
     CDS             2821..4500
                     /gene="ilvD"
                     /locus_tag="VIG70_13605"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY8251636.1"
                     /translation="MAYNRRSRLITAGVARSPNRAMLRAVGFGDGDFDKPIVGVANGH
                     STMNPCNAGIQPLIDRAMAALEDAGAKPQVFGVPTVTDGIGMGTEAMKYSLVSREVIA
                     DAIETSVNGQAMDGVLVVGGCDKNMPGGVMAMARMNVPAIYVYGGTIKPGKWKGKDLT
                     IVSAFEAVGSFTAGKLSEEDFQGIERNACPSVGACGGMFTANTMSSSFEALGVSLLGS
                     SQMAAPDAEKADSSAESARVLVQAIKNDLKPRDIITRKSIENAIALVMATGGSTNAVL
                     HYLAIASAAGVKWTIDDFERMRKRVPVLCDLKPSGRFVAVDFHRAGGVPQVLKILLEN
                     GVLHGDCMTIHGKTMAQMLKDVPAQPRGDQEVIRPWSKPMYDEGHLAILKGNLAPEGC
                     VAKITGLKNPVITGRARVFDSENACMKAIMAKKIKPGDVIVIRYEGPKGGPGMQEMLA
                     PTSALIGQGLGESVGLLTDGRFSGATWGMVVGHVAPEAYVGGTIALVKEGDSITIDAK
                     KRLLQLNVPPKELAARRKKWKAPKPRYTQGLLAKYMKLVSTASRGAITDGA"
     gene            complement(4497..5069)
                     /locus_tag="VIG70_13610"
     CDS             complement(4497..5069)
                     /locus_tag="VIG70_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY8251637.1"
                     /translation="MKSLAPWILRFWFGDGDDHGKVHKRWFEKDPTFDALVTKHYQKL
                     HQEMLEGGHREWLDEPRSCLARILVLDQFPRHIYRGSALAFSTDAFALEASKHLVERG
                     WDRELLPIERMFAYLPFEHSESLADQERACELCKALDAFPETADTLRWAVAHRDIVRR
                     FGRFPHRNAALGRANTPEEAAFLKQPGSGF"
     gene            5095..5451
                     /locus_tag="VIG70_13615"
     CDS             5095..5451
                     /locus_tag="VIG70_13615"
                     /inference="COORDINATES: protein motif:HMM:NF042483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEY8251638.1"
                     /translation="MFRRSIACLLILSIAISLPAHAAMVATSDALDRGRILTVLERAD
                     FQAQLQVYGVSPADVKARVAALTDDEAAELAARIDELPAGGVSILGVALIVFLVLLLT
                     DILGYTKVFPFTKPMK"
     gene            5460..7442
                     /locus_tag="VIG70_13620"
     CDS             5460..7442
                     /locus_tag="VIG70_13620"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY8251639.1"
                     /translation="MNRLAQETSPYLQQHAANPVDWYPWGGEALERARAEDKPILLSI
                     GYSACHWCHVMAHESFEDAGVAAVMNRLFVNIKVDREERPDLDQIYQAAQQMLSRRTG
                     GWPLTMFLAPDGTPFFGGTYFPKEARYGLPGFPDLCERVAGLWREKRAEIGAQNAEVL
                     QAFGRTLPKRAAQTRALDDAPVQALLDLLKANFDARLGGFGAAPKFPHPTDLELCLRK
                     GETGMAQITLARMCEGGIYDQLGGGFCRYSVDAEWMIPHFEKMLYDNGPLLGLLADAW
                     LATGDALYARCAGETAAWMMREMQSPGGGYYSSLDADSEHEEGKFYVWDRDEVQTLLT
                     PQEYAAAAAHFGLDQPPNFENRHWHLRVAKPMDNPILETAKTKLRAAREKRVRPGRDE
                     KVLVSWNAAAMRGMAHAGRVFGNSAWIASARGALEFIRSTMWRDGRLTATYKDGRAHL
                     NAYLDDYALLIAALLELLQAEFRVRDLDFATQLADVLIDQFEDAADGGFFFTAREHER
                     LIHRPKPAHDHATPSGNAMAAWVLGRLSALTGQERYARAAERTLELFHPAMREHPGGF
                     AMMAVALDEYLAQPKTLILRGKDDAIRTWRQELAREFLPDTEVLAIPDDSKGLPRILD
                     KPRRPQPVNAWLCRGVTCLEPISDLVNLKKTLKEKA"
     gene            7439..7738
                     /locus_tag="VIG70_13625"
     CDS             7439..7738
                     /locus_tag="VIG70_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8251640.1"
                     /translation="MKRVVLILGLLAAFPAAASDELAKKHACFACHTLDKKMVGPSYK
                     EVAAKYRSDKEAAAKLAAKVKNGGQGVWGNVPMPPNAAVPDADIKALVGWILSQK"
     gene            complement(7701..8231)
                     /locus_tag="VIG70_13630"
     CDS             complement(7701..8231)
                     /locus_tag="VIG70_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8251641.1"
                     /translation="MISPVHAQTMAAYNRWMNERLYELCTGIPESERKRDRGAFFRSV
                     HGTLNHLLYGDRAWMSRFTGRDLGWRGPADELYADFAELRAARVGLDRLIEDWAARLD
                     AQWLAADFTYTSRIDGRTRTLPAWLLVTHLFNHQTHHRGQLTTLLSQLGIDPGVTDLP
                     WLPGLTSGKGSSPPEP"
     gene            8300..>9433
                     /locus_tag="VIG70_13635"
     CDS             8300..>9433
                     /locus_tag="VIG70_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004541890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8251642.1"
                     /translation="MKKLQPGTTLIATAIAAAFTLAGCGPSGSGGGGAEVRIGHVGPL
                     TGGIAHLGKDNENGARLAVEEANAAKIKIDGKEVKFVFVAEDDQADPKVGATVAQKLV
                     DAKVAGVVGHLNSGTSIPASPIYSQAGIPVISGSATNPKLTEQGFKTQFRVVGRDDQQ
                     GPAIASYLADNNKPKTVAVIDDATAYGEGIANEVEKTLKAAKITVLPREKGTDKTTDW
                     KAILTKLRGRNPDAVFYGGMDATGGPLMKQGRELGIKAVFAFGDGACTDEMAKLAGES
                     AEGLLCSQAGIPVAAADKKFLDAYKKKFNADPILYSPFTYDAANMLIQAMQKANSSDP
                     KKYLPELAKLDFKGVTGKIAFDSKGDRKDAEMTIFTMKKGKIEP"
BASE COUNT         1788 a         3026 c         3209 g         1410 t
ORIGIN      
        1 cgccggcacc atgtgccctt cgtatcgcgc gacgatggac gagcgccatc tcacccgcgg
       61 ccgggcgaat acgttgcgtc ttgctttgtc caaccagctc ggtgatcagg gtgatgaagc
      121 agtaaaggaa gccctggacc tctgcgtctc gtgcaaaggc tgcaagcgcg aatgcccgac
      181 cggcgtcgac atggcgcgca tgaagatcga gtttctcgcg cgctacaaag cgacccatgg
      241 gctctcggcg cgcgaccgcg cgatcggcta cctgccgcgc tatgcgcctt gggcggcgcg
      301 gttggcaccg ttatcgaatc tggtttcttc cttgtcgaaa tcgtttctcg gcttcgccac
      361 gaagcgcgag ctaccgaaat ggcgcggcga cttcttcacc gagcaaggaa gaagcgacgg
      421 tgacgtggtg ctcttcgtcg acacgttcaa ccgatacttc gagccggaga atgcgcgcgc
      481 ggcgctcaag gtgctcgagg ccgcaggaca tcgcgtgcac gctccccgag gcttgtgctg
      541 cggccgcacc ttcctttcgg tgggactcgc cgacgaggcg aagcgcgagg cccggaaaat
      601 gctcgacgca ctttcgccgt atgcggcaaa gggaattccg atcgtcgggc tcgagccctc
      661 gtgcctgttc tcgctgcgcg acgagttttc tgttttggtg aagaacggca atgcgctagc
      721 gagcagctct gtcctgctcg aagagttcct cgcgcgcaat cccgggaagc tgaagtttcg
      781 cgagatgaag gcggacgtgc tgctgcacgg ccattgccac cagaaggcgt tcgatgcgat
      841 gcccgcggtg gaaaaggtgc tcggcatgat tccagggctg aaggcctcga ccgtgcaaac
      901 cagctgctgc ggcatggcgg ggagcttcgg ctacgaggcg gagcactacg acgtctcgat
      961 gaaaatggct gaagccgcgc ttcttccggc gatcaggaaa tcacccaatg ccctgctggt
     1021 tgccgatggc acgagctgcc gccaccagat cgccgacggc gcggggcgcg aagccgtgca
     1081 cgtggcacga gtgctcgcgg actgcctagc ctagccgcgc gaaaaacgcc atcaccagcg
     1141 acaccgtaac gaccgaggcc gcggtcgaga tcaccgcgcc ggtcgaggtc tccgcctcga
     1201 gcagcttgta gcgctgggcg aggacgaaag cggtggcgcc cagcggcagc gccgcgcaga
     1261 cgaccgcgat cttcgcccag aacggatcga cgggcaggat ccagaacgca gccgctgcca
     1321 tcaagagagg gtgaagcacg agcttgagca cgctcgtctg ccagctcgac gcggcgccgg
     1381 cgcggatcga gagctgcgag acgaacagtc cgatggcgaa cagcgcgcac gggcctgccg
     1441 ccgcagcgag cagggaagcg aaacgctccg cgggtaaggg gatcttcact tccaatgcga
     1501 caagaacgaa tgcaagcgca atcggccaaa tgagcgggct tctggcgacg ccgcccaggg
     1561 cacggcgcac gccgccgcgc cgcgggttga cgaagagctc gagacagacg atcgcgagcg
     1621 caaaggagac gatgttggtc gcaaccgtgg cgagcgcggc gggcaacgcc gcacgctcgc
     1681 cgtaggcgac gctgacgagc ggtatgccga gatagccggt gttcgcgaag gaagcgttca
     1741 gagcgcgcat cgtgctttcc gcgggacgcg acccggcagc atgcgagagg aagaatcccg
     1801 cgacggcagt ggcaagcgtc gcgccgatga cggcaagcag aaagaagccg ctcagaatct
     1861 cctccagcga gccgcgatac accgccacga acagcatcgc cggcagggcg aaggcgtaga
     1921 cgaactggtt gagcgccagg ctcgaggcct gggtgacgat gtggaagcgg ccggcgaggt
     1981 agccgcaggc gaccagcccg aagaccggga gcaccgcgcc gagaaccggg tctagcgtcg
     2041 actccacgcc aggagcgcgg cgccgcctgc gatcatcgcg aggcagagcc attgccccat
     2101 cgtgagcccg agcgccaggt acccgatgaa ggcgtcgggc tcgcggccga actcggcgac
     2161 gaagcgcagc accccgtagc cgagcaggaa catcgccgag acctggccgc gcgcgcgcgg
     2221 cttcgacgag aaccaccaca ggaaggcgaa cagcaccacg ccctcgagcg cgaactggta
     2281 gagctgggag gggtggcgcg gcgcgtcgcc ggcgccgggg aacaccatgc cccagggcag
     2341 atcggtgacg cgtccgggca gctcggcgtt gatgaaattg ccgagcctgc cggcggcgaa
     2401 ccccagcgga cacagcggcg cgaggaagtc catgaggcgc agccagtcga cgccgtggcg
     2461 gcgcgcggcg aacaccatcg cgagcatcac gccgagcaga ccgccgtgga acgacatgcc
     2521 accctgccag atgtagaaga tctcgagcgg gtgtgaaaag tagtgcccgg gcttgtagaa
     2581 cagcacgtag cccaggcgcc cgccgagaat cacgccaagc acgatgagga acaggaggtc
     2641 gtcgagctgt gcccgggtga tcggcgcttc gcctttggcg atcctcctca atcccagcca
     2701 ccagcccgcc gcaaagcccg cgaggtacat caggccgtac cagcgcacgg cgagcggccc
     2761 gatcgagaac gcgatcggat cgatattagg atggatcagc acgttagaat ttcattctaa
     2821 atggcttaca acagacgcag ccggctgatt accgcgggcg tagcccgctc ccccaaccgc
     2881 gcgatgctgc gagcggtcgg cttcggcgac ggcgatttcg acaagccgat cgtcggcgtg
     2941 gcaaacggcc actcgacgat gaatccctgc aatgccggca tccagccgct catcgaccgc
     3001 gccatggcgg cgctggagga cgcgggcgcc aagccgcagg tgttcggcgt gccgacggtc
     3061 accgacggca tcggcatggg caccgaggcg atgaagtact cgctggtgtc gcgcgaggtg
     3121 atcgcggatg cgatcgagac ctcggtcaac ggccaggcga tggacggggt gctggtggtc
     3181 ggcggctgcg acaagaacat gcccgggggc gtgatggcga tggcgcgcat gaacgtgccg
     3241 gcgatctacg tctacggcgg caccatcaag cccggcaaat ggaaaggcaa ggacctgacg
     3301 atcgtctcgg cgttcgaggc ggtcggctcg ttcaccgcgg gcaagctgag cgaggaggat
     3361 ttccagggca tcgagagaaa cgcgtgcccg agcgtcggcg cctgcggcgg catgttcacc
     3421 gcgaacacca tgagctcctc gttcgaggcg ctgggcgtga gcctgctcgg ctcgtcccag
     3481 atggcggcgc cggatgccga gaaggcggac tcctccgccg agtcggcgcg cgtgctggtc
     3541 caggcgatca agaacgacct caagccgcgc gacatcatca cgcgcaagtc gatcgagaat
     3601 gcgatcgcgc tggtgatggc gacgggcggc tcgaccaacg cggtgctcca ctacctcgcc
     3661 atcgcctccg cggcgggcgt gaagtggacc atcgacgact tcgagcgcat gcgaaagcga
     3721 gttccggtgc tgtgcgacct caagccctcc gggcgcttcg tcgcggtgga cttccaccgt
     3781 gccggcggcg tgccgcaggt gctgaagatc ctgctcgaga acggcgtgct gcacggcgac
     3841 tgcatgacca tccacggcaa gacgatggcg caaatgctga aggacgtgcc ggcgcagccg
     3901 cgcggcgacc aggaagtgat ccgtccctgg tcgaagccga tgtacgacga ggggcacctc
     3961 gcgatcctca agggcaacct ggcccccgaa ggctgcgtcg ccaagatcac cggactcaag
     4021 aatccggtga tcacggggcg ggcgcgcgtg ttcgactccg agaacgcgtg catgaaggcg
     4081 atcatggcca agaagatcaa gcccggcgac gtcatcgtga tccgctacga gggacccaag
     4141 ggcggaccgg ggatgcagga aatgttagcg cccacttcgg cgctgatcgg gcaggggctg
     4201 ggtgagtcag tcggcctcct caccgatgga cgtttttccg gcgcaacctg gggcatggtg
     4261 gtcgggcacg tggcgcccga agcctatgtc ggcggcacga tcgctctggt gaaggaaggc
     4321 gactcgatca ccatcgacgc caagaagcgg ctgctgcagt tgaacgtgcc gccgaaggag
     4381 ctcgcggcgc ggcgcaagaa gtggaaggcg cccaagccgc gctacacaca ggggctgctc
     4441 gcgaagtaca tgaagctggt gtcgaccgct tcgcgcggcg caatcacgga cggcgcctag
     4501 aagccggacc cgggctgctt cagaaacgcg gcttcctccg gcgtgtttgc acggccaagc
     4561 gcggcgttgc gatgggggaa gcggccgaag cggcgcacga tgtcgcgatg agcgaccgcc
     4621 cagcgaaggg tgtccgcggt ctccggaaag gcatcgagcg ccttgcatag ctcgcaggcg
     4681 cgctcctggt cggcgagcga ttcgctgtgc tcgaacggca ggtacgcgaa catgcgctcg
     4741 atcggcagca gctcgcgatc ccagccgcgc tcgacgagat gcttcgacgc ttcgagggca
     4801 aacgcgtcgg tcgagaaagc gagcgccgag ccgcggtaga tatgccgcgg aaactggtcg
     4861 agaacgagga tgcgcgcaag gcacgagcgc ggctcgtcca gccattcgcg gtgcccgccc
     4921 tcgagcatct cctggtgcag cttctggtag tgcttcgtca ccagggcatc gaaggtcgga
     4981 tccttttcaa accagcgctt atggaccttt ccatggtcgt cgccatcgcc gaaccagaag
     5041 cgcaatatcc acggtgctaa gcttttcatc gaccccccat caaaggagcc ctccatgttc
     5101 cgtcgttcga tcgcctgcct gctgatccta tcgatcgcca tttcgctgcc cgcccacgcc
     5161 gccatggtcg caacctctga cgccttggac cgggggcgga ttcttaccgt gctcgagcgc
     5221 gccgacttcc aggcgcagct acaggtgtac ggcgtcagtc cggccgacgt caaggcgaga
     5281 gtggcggcgc tgaccgacga tgaagccgcc gagctcgccg cgaggatcga cgaacttccg
     5341 gcgggtggcg tcagcattct gggcgtggcc ctcatcgtgt tcctagtgct cctgctcacg
     5401 gacattcttg gctataccaa ggtatttccg ttcaccaaac cgatgaagta gaccgcctcg
     5461 tgaatcgcct tgcgcaggag acctcgcctt acctgcagca gcacgctgcc aacccggtcg
     5521 actggtatcc ctggggaggc gaggcgctgg agcgcgcgcg cgcggaggac aagccgatcc
     5581 tgctgtcgat cggctattcc gcgtgccact ggtgccacgt tatggcgcac gagagcttcg
     5641 aggatgccgg cgtcgctgcg gtgatgaacc gcctgttcgt caacatcaag gtggatcgcg
     5701 aggagcgccc ggacctcgac cagatctatc aggccgcgca gcaaatgctc tcccggcgca
     5761 ccggcggctg gccgctcacc atgttcctcg cgcccgacgg gacgccgttc ttcggcggca
     5821 cgtacttccc gaaggaggcg cgctacggcc tgccgggatt tcccgatctg tgcgagcgcg
     5881 tcgccgggtt gtggcgcgag aagcgcgccg agatcggagc gcagaacgcg gaggtgctgc
     5941 aggccttcgg gcgcacgctg ccgaaacggg ctgctcaaac cagggcgctg gatgacgcgc
     6001 cggttcaggc cctgctcgat ctgctgaaag ccaatttcga cgcgcgcctg ggcggcttcg
     6061 gcgcggcgcc caagttcccg catcccacgg atctcgagct gtgcctgcgc aagggcgaga
     6121 ccgggatggc gcagatcacg ctggcgcgca tgtgcgaagg cgggatctac gaccagctcg
     6181 gcggcggctt ctgccgctac agcgtcgatg ccgagtggat gatcccgcat ttcgagaaga
     6241 tgctttacga caacgggccg ttgctcggct tgctggccga cgcctggctc gcgacgggcg
     6301 acgcgctcta cgccaggtgc gccggcgaga ctgcggcctg gatgatgcgc gagatgcagt
     6361 cgccgggagg cggctattac tcgtcgctcg acgccgacag cgagcacgag gaaggcaagt
     6421 tctacgtctg ggaccgcgac gaagtgcaga cgcttctgac gccgcaagag tacgccgccg
     6481 ccgcagcgca tttcgggctc gaccagccgc cgaacttcga gaaccggcat tggcacctgc
     6541 gtgtggcgaa gccaatggac aatccaattc tcgaaacggc gaaaacgaaa ctccgggctg
     6601 cacgggaaaa gcgagtgcgg ccggggcgcg acgaaaaggt cctggtgtcg tggaatgcgg
     6661 ctgcgatgcg cgggatggcg cacgccgggc gcgtgttcgg caactcggcg tggatcgcgt
     6721 ccgcgcgcgg tgcgctggag ttcatccgct cgacgatgtg gcgcgacggg cggctgaccg
     6781 cgacctacaa ggatggccgc gcgcatctca acgcctacct cgacgactat gccctgctca
     6841 tcgcggcgtt gctcgagctg ctgcaggcgg agtttcgggt cagggacctg gatttcgcta
     6901 cgcagctcgc cgacgttctc atcgatcaat tcgaggacgc cgccgacggc gggttttttt
     6961 tcaccgcgcg cgagcacgag cggctgatcc accggccgaa gccggcccac gaccacgcca
     7021 cgccgtcggg aaacgcgatg gccgcctggg tgctcggccg gctctccgcg ctgaccggcc
     7081 aggaacgcta cgcccgcgcg gccgagcgca cgctcgagct cttccatcct gcgatgcgcg
     7141 agcacccggg cgggttcgcg atgatggcgg ttgcgctcga cgaatacctc gcgcagccca
     7201 agacgctcat tctcaggggc aaagacgatg caatccggac ctggcggcaa gagctggcgc
     7261 gcgagttcct gccggatacg gaagtgctgg ccatcccgga tgattcgaag ggactgcctc
     7321 ggattctcga caagccgcgg cgtccgcagc cggtcaacgc atggctgtgc cgcggcgtta
     7381 cctgcctgga gccgatcagc gatctagtaa acttgaaaaa aaccctgaag gagaaggcat
     7441 gaagcgcgtt gtactgattc tgggcttgct cgctgcgttt ccggccgcgg cgagcgacga
     7501 gctggcgaag aagcacgcct gcttcgcctg ccatacgctg gacaagaaga tggtcggccc
     7561 gtcctataag gaggtcgccg ccaagtatcg cagcgacaag gaagccgccg ccaagctcgc
     7621 cgcgaaggtc aagaacggtg gccagggggt atggggaaac gtgccgatgc cgccaaacgc
     7681 cgcggtgccc gacgcggaca tcaaggctct ggtgggctgg atcctttccc agaagtaagc
     7741 ccgggaagcc agggcaggtc agttaccccg gggtcgatgc caagctgcga cagcagcgtg
     7801 gtgagctgtc cgcgatggtg cgtctggtgg ttgaacaggt gggtgacgag gagccaggcc
     7861 ggcagcgtgc gcgtgcggcc gtcgatccgc gaggtgtagg tgaagtccgc cgcgagccac
     7921 tgggcgtcca gccgcgctgc ccagtcctcg atcagccggt cgagccctac gcgcgccgcg
     7981 cgcagctcgg cgaagtcggc gtacagctcg tcggccgggc ctcgccagcc gaggtcgcgg
     8041 ccagtgaagc ggctcatcca ggcgcggtcc ccgtagagca ggtggttcag ggtgccgtgg
     8101 accgacctga agaaggcgcc gcgatcgcgt tttcgctcgc tctccgggat gccggtgcac
     8161 agctcgtaca ggcgctcgtt catccatcgg ttgtaggccg ccatcgtttg ggcgtgaacc
     8221 gggctgatca tcgtagtttc ccccagtgcg accgcggtat gatatcggcc ctcggcacga
     8281 accccccttg ggagacccca tgaagaagct tcaacccggc actaccctca ttgcaacggc
     8341 gatcgccgcc gctttcaccc tggccggctg cggcccgtcc ggcagcggcg gcggcggcgc
     8401 tgaagtcagg atcggccacg tcggtccgct caccggcggc atcgcccacc tgggcaagga
     8461 caacgaaaac ggcgcgcgcc tcgcggtcga ggaagcgaat gcggcgaaga tcaagatcga
     8521 cggcaaggaa gtgaagttcg tcttcgtcgc cgaggatgac caagccgacc cgaaggtcgg
     8581 cgccaccgtg gcgcagaagc tggtcgatgc caaggtcgcc ggggtcgtcg ggcacctcaa
     8641 ctccggcacc tcgatcccgg cctcgccgat ctacagccag gcgggcatcc cggtgatctc
     8701 cggctcggcc accaatccca agctcaccga gcagggcttc aagacccagt tccgtgtcgt
     8761 cggccgagac gaccagcagg gcccggcgat cgcgagctac ctcgcggaca acaacaagcc
     8821 caagacggtc gcggtaatcg acgacgccac cgcgtacggc gaaggcatcg ccaacgaggt
     8881 cgagaagacc ctcaaggcgg cgaagatcac cgtgctgccg cgcgagaagg gcaccgacaa
     8941 gacgaccgac tggaaggcga tcctgaccaa gctgcgcggc cgcaaccccg acgccgtgtt
     9001 ctacggcggc atggacgcga ccggcgggcc gctgatgaag cagggccgcg agctcggcat
     9061 caaggcggtg ttcgcgttcg gcgacggggc gtgcaccgac gaaatggcga agctcgccgg
     9121 cgagtccgcc gaagggctgc tgtgctcgca ggccggcatc ccggtcgcgg ccgcggacaa
     9181 gaagttcctc gacgcctaca agaagaagtt caacgccgat ccgatcctgt attcgccgtt
     9241 cacctacgac gccgccaaca tgctgatcca ggcgatgcag aaggcgaact cgagcgatcc
     9301 gaagaagtac ctgccggagc tggccaagct cgacttcaag ggcgtcaccg gcaagatcgc
     9361 attcgacagc aagggcgacc gcaaggacgc cgagatgacg atcttcacca tgaagaaggg
     9421 caagatcgag ccg
//
LOCUS       DATBSZ010000217        11739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1131468, whole genome shotgun sequence.
ACCESSION   DATBSZ010000217 DATBSZ010000000
VERSION     DATBSZ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11739
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131468"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VIG70_13640"
     CDS             complement(<1..968)
                     /locus_tag="VIG70_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8251643.1"
                     /translation="MGIDEVRRENRHIALGGLRDALVMMVDDDSMVIELTQAFLQDAG
                     YRRFVSTSDSSGAVAMLLRELPDVVLVDLNMPHVSGFDVLASMRADPVLKHIPAIVMT
                     SADDPETRLKALDLGASDFLRKPVDPSELALRLRNTLAAKAHREAIRKAFVRYVSPRL
                     AEGIINDAAAPFSSPAQRAEMVALFADLRGFTRITETIGVEHVVEMLNEYFTVLTEAA
                     YQHDGTIFNMAGDSLLVGFNVPFLQADAAARAWRTAVDMTARFGVVAAGWRKRHGVDT
                     GVGIGICQGPVIIGNVGSPHYMSYTVIGNPVNTAARLMEAAQMGEVL"
     gene            1176..1412
                     /locus_tag="VIG70_13645"
     CDS             1176..1412
                     /locus_tag="VIG70_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251644.1"
                     /translation="MSKILALAACCAIMAGCAGSGGIDCSADWYSIGQRDGRLGADSQ
                     AERYAARCSGQVDRQRYEEGLSAGLAMRPRIPSF"
     gene            complement(1409..2206)
                     /gene="iolB"
                     /locus_tag="VIG70_13650"
     CDS             complement(1409..2206)
                     /gene="iolB"
                     /locus_tag="VIG70_13650"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEY8251645.1"
                     /translation="MSRLLVKPRTDHGQVIHITPQSAGWRHVGFDVHKLAPGDHAAAA
                     AGNREVCLVLVSGKARVKAGGRDFGVLGERMTPFEGKPHAVYVPGGADWRVEASSDLT
                     LAVCSAPSGGGKHPARHIAPQDMGQETRGKGTNVRYVTNILPEDAPADSLLVVEVLTP
                     GGHTSSYPPHKHDQDNLPAESLLEETYYHRLNPAQGFAFQRVYTDDRSLDEAIVVEDG
                     SVVMVPRGYHPVAAIHGYDLYYLNVMAGPRRTWKFHNAREHEWLMKP"
     gene            complement(2199..4091)
                     /gene="iolC"
                     /locus_tag="VIG70_13655"
     CDS             complement(2199..4091)
                     /gene="iolC"
                     /locus_tag="VIG70_13655"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEY8251646.1"
                     /translation="MLKLDVISIGRCSVDLYGQQIGSRLEDIATFAKSVGGCPANIAI
                     GTARLGLKSGLVTRVGGEQMGRFVIEQMRREGVAVDGIRIDPERLTALVLLAVEDEGV
                     SPMIFYRADCADMALSEEDIDEAYIASARAIVVTGTHFSRPNSDAAQRKAIRIAKANG
                     RKVVFDIDYRPTLWGLTGHAAGFERYVRSEKVSNQLRTVLPDCDLVVGTEEEIMIAAG
                     ADSAAAALKAIRAVSRATIVLKRGPMGCIVYDGAIGELEDGIVGKGFPIEVYNVLGAG
                     DAFMSGFLRGWLKGEPLATAATWANACGAFAVSRLLCSPEYPTWAELSHFLEKGSPQR
                     ALRKDAAINHLHWATTRRDAPHDLMALAIDHRAQLEAIADRHGAPRSRLEQFKLLAVE
                     AATKVSRGRPGFGMLLDSTYGQKALYKAGKAGLWIGRPVEKPGSRPLDLEPMHLAEWP
                     VTHTVKCLCFYHPDDSAELKALQERELTRVQDAARTAGRELLLEIIAGKHGPLAETTV
                     ASVLGRLYALGIKPDWWKLEPQKSPGAWRAIEQVIGANDPYCRGIVLLGLEAPQAELA
                     SAFRMASGTKLVKGFAVGRTIFADAAEKWLSGSMTDEDAVADMAARFEALVSAWQSTR
                     KEIQHV"
     gene            complement(4084..4923)
                     /locus_tag="VIG70_13660"
     CDS             complement(4084..4923)
                     /locus_tag="VIG70_13660"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEY8251647.1"
                     /translation="MSDAERLTRDWWRTGIIDMSPGVIRFRGYAIEELIGRISFTQMI
                     WLMTRGELPSRAQGELLEAALTAGVDHGPQAPSIAIARMAATCGVGLNSAVASALNVL
                     GDVHGGAGEQTVELLHAIAERIARGEAESAAVQAALAEFNARHGKFIPGYGHRFHPVD
                     PRAPRLLELVDAAAREGVVDGRFARIGRAVEAALAQRKSGKRIPMNIDGATAVIYAEL
                     GFPAPLARGLFCLSRAVGILAHAWEQTLQGGRNKGPMPREYLPIYDGPAPRRLGDAKL
                     GNP"
     gene            complement(4920..5414)
                     /locus_tag="VIG70_13665"
     CDS             complement(4920..5414)
                     /locus_tag="VIG70_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251648.1"
                     /translation="MLNEPAVLPLPEAVRAAADEGAVLQEEFERAQRVASGQIGAQDL
                     DWLSKGFAAFLANGGALPLERCLRLPWKDGALRRACRDYWLRRAWKLVGGDLSPWRRS
                     EVLAATVRNFSSRQWGRWRGLQAVPSEAGDLESALFHAFRASERIPTTAMQLHNIAHH
                     RRHT"
     gene            complement(5532..7358)
                     /locus_tag="VIG70_13670"
     CDS             complement(5532..7358)
                     /locus_tag="VIG70_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY8251649.1"
                     /translation="MNQPRDLKGMNQAATSDLVSHQLVKYLEERGVEHVFGLCGHTNI
                     AVLAELAKSSIKFINTRHEQVAAHMADGYARAKRQTAVVLSHLGPGLTNASTGVANAS
                     LDSIPMVVIAGDVPSHYYGKHPHQEVNLHADAAQYEIYRPFVKRAWRVERPDLFPEII
                     DKAFQLAGSGRPGPVLVSVPMDIFSKPVDPALFQRLAHHTRSLHKPSIDDETAAGMVK
                     ALAGASRPVIYAGGGVLLADAADELRDFAAHFSIPVAHSLMGKGALPDDHPLTLGMTG
                     FWGTEFINRKCREADYVLGLGTRFAEADCSSWDPRFTFSMPPTRLVQIDIDPAEIGRN
                     YPVEIGAVADLKQALRVMLRVAKQLYPAGRRNDALVKEIAANRRTFAQGNEAHIRNEG
                     YPMRPERILAEVREVLPRDALITTDVGWNKNGVGQQFPVYTPGTIFTPGGYATMGFGA
                     PAALGVKLACPDRTVVALVGDGGFGQNPAMLATAVEADIPVVWVVMNNYAFGTIAGLE
                     MAHYGTAFGCVFRKDGKPYSPDFAAIARAYGAEGVQIAAAREFKPALERAVRSSRPCV
                     IDVAMLNEPVPTGGHWNINDIYSPGRKASHVAIDYAATPAAK"
     gene            complement(7375..8577)
                     /locus_tag="VIG70_13675"
     CDS             complement(7375..8577)
                     /locus_tag="VIG70_13675"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8251650.1"
                     /translation="MTQALSGIRVLDLSNVLAGPLCAYQLALLGAEVIKVEVPKTGDL
                     ARQLGADPALNARHMGASFLAQNGGKKSITVNLKSEPGKEVLRRLVRTADVLVENFRP
                     GVMDRLGLGYRELREINPSLVYCAISGFGQSGPMKDAPAYDQIIQGLSGMMSVTGNDD
                     CGPMRAGYPVADTIGGITAAFAIAAALVRRSRSGEGSLIDVSMLDSAIVSLGWVVSNY
                     LIAGVTPRAIGNENFTAAPSGTFRTRQGLLNIAANKQEQFEALAKVLGREELLADPRF
                     AAREARKCNRAALTRELEAALAERSAAEWETALNRLGVPAGRVLTVPEVLEVAQVKER
                     ELLQVFDNAPGVDRSITLARCGFKMSDGDPSVSAPPPQLGEHTAEILHAIGYSSADIG
                     RLRQAGAV"
     gene            complement(8585..10105)
                     /locus_tag="VIG70_13680"
     CDS             complement(8585..10105)
                     /locus_tag="VIG70_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY8251651.1"
                     /translation="MLGNTEVAGRKVQASEQLRRYDHHIGGAAVPPGSRAYLPTEDPF
                     TGKTWALIARGNTADAHAAVEAADAAFQGPWSRLSATERGHLLWKLGERVQAEAPRLA
                     EIERRDNGKLAVEVLSQVKYMGDYFKYYAGLADKVQSQVIPTDKKGVFTYTRYEPKGV
                     VVIITPWNSPLTLTSWKLAPALAVGCTAVIKPSEFTSASMLELAELFDQVGFPKGVVN
                     VVTGLGPEVGEALVTHPKTAHIGFTGGDAAGRKIYELAARHLKTVTLELGGKSPNIVF
                     DDADLPQAVKGVVSGIFAASGQSCQAGSRLLVQRTIHDRFVQMLADFVRSAKLGDPAA
                     ADTQIGPIATRPQFEKILRYIDIAKQEGARCVLGGASRPELGAGLFVEPTIFTGVRND
                     MRIAQEEVFGPVLAVIPFEDEADAIRIGNDVAFGLAAAVWTRSLHRAMLLTEKLRAGT
                     VWVNNYRATSFTSPFGGYKNSGIGRESGTEAIREYLHTKTVWISTDLDVPNPFVRR"
     gene            10277..11080
                     /locus_tag="VIG70_13685"
     CDS             10277..11080
                     /locus_tag="VIG70_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY8251652.1"
                     /translation="MPAAAGVAAVDRALAILDAFTDNDRKLTLAELAKRTGLYKSTVI
                     RLAKSLEKARYLLRAEDGTYRLGSKVLSLGAQYQKNFGTAEIVPQTLRQIVDELHEGA
                     SFYVRDEAQRLCLHRIESPRAVRDSIHVGDRLPLTVGAAGHVILAFSGLTGERYDLIR
                     KNMYAASFGERDPETAAVACPVLGPDQKFVGALSVSGPRYRIEAMGVQNILPVLFHHA
                     RNLCRILGGDPDAPQFAAWSKPAKVLRAQHAPGAAKGYRAPASGARAGR"
     gene            11284..>11739
                     /locus_tag="VIG70_13690"
     CDS             11284..>11739
                     /locus_tag="VIG70_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251653.1"
                     /translation="MLGGLSTAVLANGDRGSSTRSGSNPDLNDPKISGDFDYGVCRGT
                     NPKCYHDWAHEERDDPPRVLIYTRTAGPRHANLGPRLAAGLNPPLTPEGCVPAPGVSC
                     NDVQHALIRWLKAKGVEADWTEDVTRITNLHRYRAVIFASTSRDTLFAHG"
BASE COUNT         1781 a         4048 c         4007 g         1903 t
ORIGIN      
        1 agcacctcac ccatctgcgc agcctccatg aggcgcgccg cggtgttcac gggattgccg
       61 atcacggtgt agctcatgta gtgcggcgag cccacgttgc cgatgatcac cgggccctgg
      121 cagatgccga tgccgacgcc ggtgtcgacg ccgtgccgct tgcgccagcc ggccgccacc
      181 accccgaagc gcgccgtcat gtccacggcg gtgcgccacg cgcgcgccgc ggcatcggcc
      241 tgcaggaagg ggacgttgaa gcccaccagc aggctgtcgc ccgccatgtt gaagatcgtg
      301 ccgtcgtgct ggtaggcggc ctcggtcagc accgtaaagt attcgttcag catctcgacc
      361 acgtgctcca cgccgatggt ctcggtgatc cgggtgaagc cgcgcaggtc ggcaaacaga
      421 gccaccatct cggcgcgctg cgccggggag gagaacggcg ccgcggcatc gttgatgatg
      481 ccctcggcaa ggcgcggcga gacgtagcgc acgaacgcct tgcggatcgc ttcgcggtgc
      541 gccttggccg ccagggtatt tctcagccgc agcgcgagct cgctgggatc gaccggcttg
      601 cgcaggaaat cgctcgcccc gaggtcgagc gccttcagcc gcgtttcggg gtcgtccgcg
      661 gaggtcatga cgattgccgg aatgtgcttg agaaccgggt cggcccgcat gctggcgagc
      721 acgtcgaagc cgctgacgtg gggcatgttg aggtcgacca gcacgacgtc cggcagctcg
      781 cgcagcagca tggcgacggc gccggaggag tcggaggtgg agacgaaccg ccggtatccc
      841 gcgtcctgca ggaacgcctg ggtgagctcg atcaccatcg aatcgtcgtc gaccatcatg
      901 acgagggcgt cgcgtagccc gccgagcgcg atgtgcctgt tttcgcggcg gacctcgtcg
      961 attcccatcg tttcattcat accatggaac acgcagaacc ctttttatga caacccggtc
     1021 acgtgcatcg cgccgcggct cggctctcgc tgcggcgcta cgagcctgaa cttagcaggg
     1081 ccctgaaaga gcgggggtta aggtctgtca tcgggagcca cggcagcgtg acgcagttcg
     1141 cggaatagcg gtcgatgtcg cgctgtatgc aggcgatgag caagattctt gcactcgcgg
     1201 cttgctgcgc aatcatggct ggctgcgccg gcagcggcgg aatcgactgc agcgccgact
     1261 ggtattcgat cggccagcgc gacggccggc tcggcgcgga ttcgcaggcc gagcgttatg
     1321 ccgcccgttg ctccgggcag gtcgaccggc agcgctacga ggaaggcttg tcggcgggcc
     1381 tggcgatgcg cccgcgcatc ccgagcttct agggtttcat gagccactcg tgctcgcgcg
     1441 cgttgtggaa tttccacgtg cgccgcgggc cggccatgac gttcaggtag tacaggtcgt
     1501 agccgtggat ggcggcgacg gggtgatagc cccgcggcac catcaccacg ctgccgtcct
     1561 cgaccacgat cgcctcgtcg agcgagcgat cgtcggtgta gacgcgctgg aaggcgaacc
     1621 cctgggccgg gttgaggcga tggtagtagg tctcctcgag cagcgattcc gcgggcaggt
     1681 tgtcctggtc gtgcttgtgc ggcgggtagc tcgacgtatg gccgcccggc gtgaggactt
     1741 cgaccacgag cagcgagtcc gccggcgcgt cttccggcag gatgttggtg acgtagcgga
     1801 cattggtgcc tttgccgcgg gtctcctgcc ccatgtcctg cggcgcgatg tgccgcgccg
     1861 gatgcttgcc gccgcccgac ggggcggagc agaccgcgag cgtgaggtcg ctcgacgctt
     1921 ctacccgcca gtcggcgccg ccggggacgt agaccgcgtg cggcttgccc tcgaacggcg
     1981 tcatccgctc gccgagaacg ccgaaatccc tgccgcccgc cttcactctt gccttgccgg
     2041 agacgagcac caggcagacc tcgcggtttc ccgcggccgc ggctgcgtgg tcgccaggcg
     2101 cgagcttgtg aacgtcgaac ccgacatggc gccagccggc gctctgcggg gtgatgtgga
     2161 tcacctgccc gtggtcggtg cgcggcttta cgagaagtct agacatgctg gatctccttg
     2221 cgggtgctct gccacgccga aaccagcgcc tcgaatctgg ccgccatgtc ggcgacggcg
     2281 tcctcgtccg tcatgcttcc ggaaagccac ttctccgcgg cgtccgcgaa gatcgtgcgc
     2341 ccgaccgcga aacccttgac aagcttcgta ccggaagcca tgcgaaaagc gctggcgagc
     2401 tcggcctgcg gcgcctccag gccgagcagg acgatgccgc ggcagtacgg atcgttcgcg
     2461 ccgatcacct gctcgatggc gcgccatgcc ccggggcttt tctgcggctc gagcttccac
     2521 cagtcgggct tgatgccgag cgcgtagagc cgcccgagca ccgaagcgac cgtggtctcg
     2581 gcgagcggtc cgtgttttcc ggcgatgatc tcgagcagaa gctcgcggcc ggcggtgcgc
     2641 gcggcatcct ggacacgggt gagctcgcgc tcctgcagcg ccttcagctc ggcgctgtcg
     2701 tcggggtggt agaagcaaag gcacttcacc gtgtgggtga ccggccactc cgcgagatgc
     2761 atcggctcca gatcgagcgg acgcgagccc ggcttttcca ccgggcggcc gatccagagg
     2821 cctgccttgc ccgccttgta cagcgccttc tggccgtagg tggagtcgag cagcatgccg
     2881 aagccggggc gcccgcgaga caccttcgtc gcggcttcca cggcaagaag cttgaattgc
     2941 tccagccggc tgcgcggtgc gccgtgccgg tcggcgatcg cctcgagctg cgcgcgatgg
     3001 tcgatcgcga gcgccatcag gtcatgcggc gcatcgcgcc gggtcgtcgc ccagtggagg
     3061 tggttgatcg cggcgtcctt gcgcagcgcg cgctgcgggc tgcctttctc gaggaagtgg
     3121 ctgagctcgg cccaggtcgg atattccgga gagcagagga ggcgcgagac cgcgaatgcc
     3181 ccgcacgcgt tcgcccaggt cgccgcggtc gcgagcggct cgcccttcaa ccagccgcgc
     3241 agaaagcccg acatgaaggc atcgccggca ccgagcacgt tgtagacctc gatcggaaag
     3301 cccttgccga cgatgccgtc ctcgagctcg ccgatcgcgc cgtcgtagac gatgcagccc
     3361 atcgggccgc gcttgagcac gatcgtggcg cgggacacgg cgcggattgc cttcagcgcc
     3421 gccgccgcgc tgtcggcgcc ggcggcgatc atgatctctt cctcggttcc gacgaccaga
     3481 tcacaatcgg gaaggacggt gcgcagttgg ttagatactt tctctgatct gacatagcgc
     3541 tcgaacccgg cggcgtggcc ggtcaggccc cacagcgtcg gccggtagtc gatgtcgaac
     3601 acgaccttgc ggccgttcgc tttcgcaatg cggatcgctt tgcgctgcgc ggcgtcgctg
     3661 ttggggcggg agaaatgcgt gccggtcacc acgatcgcgc gcgccgacgc gatgtacgcc
     3721 tcgtcgatgt cttcttcgct gagcgccatg tcggcgcagt ccgcgcgata gaagatcatc
     3781 ggcgaaacgc cctcgtcctc gaccgccagc aggaccaggg cagtcaagcg ctccggatcg
     3841 atccggatcc cgtccaccgc gacgccttcg cggcgcatct gctcgatgac gaagcgcccc
     3901 atctgctcgc cgccgacgcg cgtgacgagg ccggatttga gccccagccg cgcggttccg
     3961 atcgcgatgt tcgccgggca gccgccgacc gacttggcga aggtggcgat atcctccagg
     4021 cgcgatccga tctgctggcc gtagaggtcg accgagcagc gcccgatgga aatgacgtcg
     4081 agcttaagga tttcctagct tagcatcacc caagcggcgc ggcgccggcc cgtcgtagat
     4141 cggcagatac tcgcgcggca tcggcccctt gttgcgtccc ccctgcagcg tctgctccca
     4201 cgcgtgcgcg aggataccga cggcgcgcga caggcagaaa aggccgcgcg cgaggggcgc
     4261 ggggaagcca agctcggcat agatgactgc cgtggcgccg tcgatgttca tcggaatgcg
     4321 cttgccgctc ttgcgctgcg cgagcgcggc ctcgaccgcg cgcccgatgc gcgcgaaccg
     4381 gccgtcgacc acgccctcgc gagcggcggc atcgaccagc tcgaggagcc gcggcgcgcg
     4441 cggatccacc ggatggaagc ggtggccgta gccggggatg aacttgccgt gccgcgcgtt
     4501 gaactccgcg agcgccgcct gcacggccgc gctttcggct tcgccgcggg cgatgcgctc
     4561 ggcgatggcg tggaggagct cgaccgtctg ctcgccggcg ccgccgtgaa cgtcgccgag
     4621 gacgttgagc gcggaggcga ccgcgctgtt caggccgacc ccgcaggtcg ccgccatgcg
     4681 cgcgatcgcg atgctgggcg cctgcggccc gtggtcgacg ccggcggtca gcgcggcctc
     4741 gagcagctcg ccttgcgcgc ggctcggcag ctcgccgcgc gtcatgagcc agatcatctg
     4801 ggtgaagctg atgcgcccga tcagctcctc gatggcatag ccgcggaagc ggatcactcc
     4861 cgggctcatg tcgatgatcc cggtgcgcca ccaatcccgg gtcaggcgtt ctgcgtcgct
     4921 caggtatgcc gccgatggtg cgcgatattg tggagctgca tggcggtcgt cgggatgcgc
     4981 tcgctcgcgc ggaaggcgtg gaacagcgcc gactcgaggt cgccggcttc ggacgggact
     5041 gcctgcaagc ctcgccagcg gccccactgc cggctggaaa agttgcgcac cgtagccgcg
     5101 aggacctcgg agcgccgcca gggcgagaga tcgccgccga ccagcttcca cgcccttcgc
     5161 agccagtagt cccggcaggc gcggcgcagc gcgccgtctt tccacggcag ccgcaggcag
     5221 cgctcgagcg gcagcgcgcc gccattggcg agaaacgcgg caaacccctt gctcagccag
     5281 tcgaggtcct gggcgccgat ctggccgctc gccacgcgct gggcgcgctc gaactcctcc
     5341 tgcagcacgg cgccctcgtc cgcggcggcc cgtaccgctt ccggcagcgg caaaaccgcc
     5401 ggctcattca gcatttctct ctcctcctcc ccaatccaag ctactcgtgc atgtctagta
     5461 catcacatca tcgttacacc aggcgcagtt cccgcgcctt caccaccgcc tgcgggcatc
     5521 tcgccccctg cctacttggc cgccggggtc gcggcatagt cgatcgccac gtgcgacgcc
     5581 ttgcgaccgg gcgaatagat gtcgttgatg ttccagtggc cgccggtcgg gaccggctcg
     5641 ttcagcatgg cgacgtcgat gacgcacggc cggctggaac gaaccgcgcg ctcgagcgcc
     5701 ggcttgaatt cgcgcgcagc ggcgatctgc acgccctcgg cgccgtacgc gcgcgcgatg
     5761 gcggcgaagt ccggcgagta cggcttgccg tccttgcgga agacgcagcc gaacgcggtg
     5821 ccgtagtgcg ccatttccag gccggcgatc gtgccgaaag cgtagttgtt catcaccacc
     5881 cacacgaccg ggatgtcggc ttcgaccgcg gtcgcgagca tcgccgggtt ctggccgaag
     5941 ccgccgtcgc ccaccagcgc cacgaccgtg cggtcgggac aggcgagctt gacgccgagc
     6001 gccgcagggg cgccgaaccc catcgtggcg tagccgccgg gagtgaagat cgtgcccggc
     6061 gtgtacaccg ggaactgctg cccgacgccg ttcttgttcc agcccacgtc ggtggtgatg
     6121 agggcgtcgc gcggcagcac ctcgcgcact tcggcgagga tgcgctcggg ccgcatcggg
     6181 tagccctcgt tgcggatgtg cgcctcgttg ccttgcgcga acgtccggcg attcgccgcg
     6241 atctccttca ccagcgcgtc gttgcgccgg ccggccgggt agagctgctt cgccacgcgc
     6301 agcatgacgc gcagcgcctg cttgagatcc gcaacggcgc cgatctcgac cgggtagttg
     6361 cggccgatct ccgccggatc gatgtcgatc tggaccagcc gcgtcggcgg catgctgaag
     6421 gtgaagcgcg ggtcccagga gctgcagtcc gcctcggcga agcgcgttcc caggccgagc
     6481 acgtagtcgg cctcgcggca cttgcggttg atgaactcgg tgccccagaa cccggtcatg
     6541 ccgagcgtga gcgggtggtc gtccgggagc gcgcccttgc ccatcaggct gtgcgcgaca
     6601 ggaatcgaga aatgcgcggc gaagtcgcgc agctcgtcgg ccgcgtcggc gagcagcacc
     6661 ccgccgccgg cgtagatcac cggacgcgat gcgccggcga gcgctttcac catgcccgcg
     6721 gcggtctcgt cgtcgatcga cggcttgtgc agcgatctcg tgtgatgcgc gaggcgttgg
     6781 aacagcgcgg ggtcgactgg cttggagaag atgtccatcg gcaccgacac cagcaccggc
     6841 ccgggccggc cgctccccgc gagctggaac gccttgtcga tgatctcggg gaagagatcc
     6901 ggccgctcga cccgccacgc gcgcttcacg aacgggcggt agatctcgta ctgcgccgca
     6961 tcggcgtgca ggttcacttc ctggtgcgga tgcttgccgt agtagtggct gggcacgtcg
     7021 cccgcgatca ccaccatcgg aatcgagtcg agcgacgcgt tcgcgacgcc ggtcgaggcg
     7081 ttggtgagtc cgggcccgag atggctcagc accacggcgg tctggcgctt cgcgcgcgcg
     7141 tagccgtcgg ccatgtgcgc cgcgacctgc tcgtggcgcg tgttgatgaa cttgatggag
     7201 ctcttcgcga gctcggcgag caccgcgata ttggtgtggc cgcacagccc gaacacgtgc
     7261 tcgacgccgc gctcctcgag gtatttcacc agctggtgcg acacgaggtc gctggtcgct
     7321 gcctggttca tgcccttgag atcgcgtggt tggttcatcg tggttctcct ttgcttagac
     7381 ggcgccggcc tggcgcaggc ggccgatgtc ggcgctgctg tagccgatcg cgtgcagtat
     7441 ttccgccgtg tgctcgccga gctgcggcgg cggcgccgac accgacggat cgccgtccga
     7501 catcttgaac ccgcagcgcg ccagcgtgat gctgcggtcc acgcctggcg cgttgtcaaa
     7561 cacctggagc agctcgcgct ccttcacctg cgccacttcg aggacttcgg ggaccgtcag
     7621 cacccggccc gccggcacgc ccagccggtt gagggccgtc tcccattccg ccgcggagcg
     7681 ctcggcgagc gccgcctcga gctcgcgggt gagtgccgcg cggttgcact tgcgggcctc
     7741 gcgcgcggcg aagcgcgggt cggcgagcag ctcctcgcgg ccgagcacct tggcgagcgc
     7801 ctcgaactgc tcctgcttgt tcgcggcgat gttcagcagg ccctggcgcg tcctgaaggt
     7861 gcccgacggc gccgcggtga agttttcgtt gccgatggcg cgcggcgtga cgcccgcgat
     7921 caggtaattc gacaccaccc agccgaggct gacgatcgcc gagtcgagca tcgacacgtc
     7981 gatcagcgat ccctcgccgc tgcggcttcg ccgcaccagc gcggccgcga tcgcgaacgc
     8041 cgcagtgatg ccgccgatgg tgtcggccac cggatagccc gcgcgcatcg gaccgcagtc
     8101 gtcgttgccg gtgacgctca tcatgcccga cagcccctgg atgatctggt cgtacgccgg
     8161 cgcgtccttc atcgggccgc tctggccgaa gccggagatc gcgcagtaga cgagcgaggg
     8221 attgatctcg cgcagctcgc ggtagccgag cccgagccgg tccatcacgc ccggccggaa
     8281 attctcgacc agcacgtcgg cggtgcgcac caggcggcgc agcacctcct tgccgggctc
     8341 cgacttgagg ttcacggtga tcgacttctt gccgccgttc tgcgcgagga acgacgcgcc
     8401 catgtggcgc gcgttgagcg cgggatcggc gccgagctgg cgcgcgaggt cgccggtctt
     8461 cggcacctcg accttgatca cctccgcgcc gaggagcgcg agctggtagg cgcacagcgg
     8521 accggcgagc acgttgctca ggtcgagcac gcggatcccg gacagggctt gcgtcatgga
     8581 cgcgctagcg gcgcacgaac ggattgggaa cgtcgaggtc ggtcgagatc cacaccgtct
     8641 tggtgtgcag atactcgcgg atcgcctcgg ttcccgattc gcgcccgatg ccggagttct
     8701 tgtagccgcc gaacggcgag gtgaagctgg tcgcgcggta gttgttgacc cagacagtgc
     8761 ccgcgcgcag cttctcggtg agcagcatcg cgcgatgcag gctgcgcgtc cagaccgcgg
     8821 cggcgagccc gaaggcgacg tcgttgccga tgcggatcgc gtccgcctcg tcttcgaatg
     8881 gaatcacggc gagcaccggg ccgaacacct cctcctgcgc gatgcgcatg tcgttgcgca
     8941 cgccggtgaa gatcgtgggc tcgacgaaca ggcccgcgcc gagctccggg cggctcgcgc
     9001 cgccgagcac gcagcgcgcg ccctcctgct tggcgatgtc gatgtagcgc aggatcttct
     9061 cgaactgcgg ccgggtggcg atcgggccga tctgcgtgtc ggccgccgcc gggtcgccga
     9121 gcttggcgct gcgcacgaag tcggccagca tctgcacgaa ccggtcgtgg atcgttcgct
     9181 gcaccagcag gcgcgagccg gcctggcagc tctgtcccga ggcggcgaag atcccggaaa
     9241 ccaccccctt caccgcctgc ggcaggtcgg cgtcgtcgaa cacgatgttc ggcgacttgc
     9301 cgccgagctc gagagtgacc gttttcaggt ggcgcgccgc gagctcgtag atcttgcggc
     9361 cggcggcgtc gcccccggta aagccgatgt gcgccgtctt cgggtgcgtc accagcgctt
     9421 cgcccacctc ggggccgagg ccggtcacca cgttgaccac gcccttggga aagcccacct
     9481 ggtcgaacaa ttcggcgagc tcgagcatcg aggccgaggt gaattccgag ggcttgatca
     9541 ccgccgtgca gccgacggcg agcgccgggg cgagcttcca gctcgtcagc gtgagcggcg
     9601 agttccaggg cgtgatgatg accaccacgc ccttcggctc gtagcgcgtg taggtgaaga
     9661 cgcccttctt gtcggtcggg atgacctggc tctgcacctt gtcggcgagc ccggcgtagt
     9721 acttgaagta gtcccccatg tatttcacct gggagagcac ttcgaccgcg agcttgccgt
     9781 tgtctcgccg ctcgatctcg gcgagccgcg gcgcttcggc ctgcacgcgc tcgcccagtt
     9841 tccacaacag gtggccgcgc tcggtcgcgc tcagccgcga ccagggcccc tggaacgccg
     9901 cgtccgcggc ctcgaccgcc gcatgcgcat cggcggtgtt accgcgtgcg atcagcgccc
     9961 aggtcttgcc ggtgaacgga tcctcggtcg gcaggtaggc gcgcgacccg ggagggacgg
    10021 cggcgccgcc gatgtgatgg tcgtacctcc gcagctgctc ggacgcttgc accttcctcc
    10081 ccgcgacctc cgtattaccc aacataacac tattccattt cacgaaacgc tcgcaagagg
    10141 gcggcaagac cggcacgcgc ggccgcggcg gcgcgccgca gcgcgctgaa atgcgctggc
    10201 aggaggaggc gggaagcacg actccgcggg gtctggtata cactggcccc ctctgaaaac
    10261 aagggttatc cagcgcatgc ctgccgccgc cggagtcgcc gccgtcgacc gcgccctcgc
    10321 gatcctcgac gcctttaccg acaacgaccg caagctcacc ctggcggagc tggccaagcg
    10381 caccggtctg tacaagagca cggtgattcg cctggcgaaa tccctcgaaa aagcgcgcta
    10441 cctgctgcgc gcggaggacg gcacctaccg cctgggctcg aaagtcctgt cgctcggcgc
    10501 ccagtaccag aagaacttcg gcaccgccga gatcgtgccg cagaccctcc ggcagatcgt
    10561 ggacgagctg cacgagggtg cctccttcta tgtgcgcgac gaggcccagc gcctgtgcct
    10621 gcaccgcatc gaatcgccgc gcgcggtgcg cgactcgatc cacgtcggcg accgcctgcc
    10681 gctcacggtg ggcgccgccg ggcacgtaat cctcgcgttc agcggcctga ccggcgagcg
    10741 ctacgacctg attcgcaaga acatgtacgc cgcctcgttc ggcgagcgcg acccggaaac
    10801 cgccgcagtg gcctgtccgg tgctcggccc ggaccagaag ttcgtcggcg cgctcagcgt
    10861 ctccgggccg aggtaccgga tcgaggcgat gggcgtccag aacatcctgc cggtgctgtt
    10921 ccaccacgcg cgcaacctgt gccgcatcct gggcggcgac ccggacgcgc cgcagttcgc
    10981 ggcctggtcc aagccggcga aagtgctgcg cgcccagcat gcgcctggcg cggccaaggg
    11041 ctaccgggcc cccgcgagcg gagcgcgcgc cggccgctag cgccccggct gttctgcgca
    11101 gcagaacagt gttgcattgt cctggctacc aaagcaccaa cccgctgacg ttttggtgca
    11161 cccgctgcta taagaaaacc agaagtcagg gcttctgaaa gaacacaccg aggagaaatc
    11221 atggacaggg aaggaagaac gatgcgcgca cgcgcaggca ctgccttagc gtccgcgctg
    11281 gtcttgctag ggggcctttc gactgccgta ctcgcgaacg gcgaccgggg cagcagcacc
    11341 cgcagcggca gcaacccgga cctgaacgac ccgaagatct ccggcgactt cgactacggc
    11401 gtgtgccgcg ggaccaaccc gaagtgctac cacgactggg cgcacgagga acgcgacgat
    11461 ccgccacggg tgctcatcta tacccgcacc gcgggccccc gccacgccaa cctgggtccg
    11521 cgtcttgctg ctggactcaa cccgccgctg acccctgagg ggtgcgttcc tgcaccgggc
    11581 gtgagttgca acgacgtgca gcacgccttg atccgctggc ttaaagccaa gggcgtggaa
    11641 gccgattgga ccgaggatgt cacgcgtatt accaacctgc accgataccg ggcggtgatc
    11701 ttcgccagca ccagccggga cacgctcttc gcccacggt
//
LOCUS       DATBSZ010000218         7485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1064180, whole genome shotgun sequence.
ACCESSION   DATBSZ010000218 DATBSZ010000000
VERSION     DATBSZ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064180"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VIG70_13695"
     CDS             <1..792
                     /locus_tag="VIG70_13695"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY8251654.1"
                     /translation="MACLGCDKDGELKRPAAHTLLSRAWSVAEAYFSADAAAAHTVEP
                     ERIEPGATFQRLARGWRISLPPQAVRWTTPPEGRDEQEIEFSWAGETARHVGTVVHHW
                     LQCIADDAMRGWDARRVESLRPLFARELECRGIQRSRAKEVAGLVADALKNSLADDRG
                     RWLLGPHPQARSEHRLRLRSTVGVRTYVLDRLFRDTAGEQWIVDFKTSRHEGGGVEEF
                     LDEQRKRYEPQLRSYAAAFDNARLGLYFPLLRGWREWLDPSTLDC"
     gene            complement(871..1833)
                     /locus_tag="VIG70_13700"
     CDS             complement(871..1833)
                     /locus_tag="VIG70_13700"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8251655.1"
                     /translation="MKTMLRGALLLLLFAANAAAQTWPAKPVRLIVADAAGGAPDQLA
                     RILSAKLAEGFGQQVIVDNRPGAGGTLGADLAAKATADGYTLLLSTTAIWAILPSLKK
                     DLPYDPVRDFAPISRLATTSNVLVINTSLPARSVAELVQLSKSKPGALNYASAGIGTP
                     AHLAGEMLNLLAGIKMTHVPYKGAAPGLLDVIAGNAHVIITSPISAGAHMNAGRVRAL
                     ATTGIERNPSLPELPPIADTVPGYEITQSWGVVAPAATPQPVLRRANEEIVRAMALVD
                     VRERVLKMGAVPAGDSPQAFEAFMARERQRLAEVITKSGIVLSE"
     gene            complement(1939..2736)
                     /locus_tag="VIG70_13705"
     CDS             complement(1939..2736)
                     /locus_tag="VIG70_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8251656.1"
                     /translation="MTHVEKIAAWLLAILWVSPLLYAFWAAFHPPEFATTFEPLAPLT
                     LANFEKAWGYAPFPRHFLNTVMLVTMILGAQLVLCTLAGFAFARFDFAGRDIAFALVL
                     LQLMIMPEVLIVENYRSMSRLGLIDTLLGIGLPYMASAFGIFLLRQTFKTIPRELEEA
                     ARVEGCSWLGVLWRVYVPLARPTYLAYALVSVSYHWNNFLWPLVVTNSVNTRPLTVGL
                     AIFGAPESGVDWSVISAGTLIAVAPLLVAFLLFQRQFMQSFMQAGIK"
     gene            complement(2733..3368)
                     /locus_tag="VIG70_13710"
     CDS             complement(2733..3368)
                     /locus_tag="VIG70_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251657.1"
                     /translation="MILALIAGAIAAASAQAQFGMPWRKTPTIVVVATEGDPRIALVD
                     EAIAYWNRLLEDTGSAFRLPAATRSALPIPEPALQEMSNAIVGVGRRPFQVPEALRNL
                     PGDLTIMLAHSGFVSFAGPFDPDGRIVVGIRGMDVWPLNLPNVARNVIAHEVGHAIGL
                     GHNPDPAMLMCGRPAPCRPPGYASERPHIFPITEEERRQLRTLYPSRGGTQ"
     gene            complement(3391..4254)
                     /locus_tag="VIG70_13715"
     CDS             complement(3391..4254)
                     /locus_tag="VIG70_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8251658.1"
                     /translation="MRHVYGWLLLAPAAILLVGFTHYPAAATLVDSFFSAGTALRPSR
                     FVGLDNYAALADDPVFWQVLRNNFWFALGTIPTSIALALLMALWVNARLPGRALVRLS
                     YFTPTVLPMIAVANLWLYFYTPQIGLLDKVTALFGVPSHNWLGNPDTVMGCVIAMTIW
                     KEAGFFMIFYLAALQSLPPELEEAARIEGASRGYFFWRVTFPLLMPTTLFVLVNATIN
                     SFKLVDHLFILTKGGPDNASNLLLYYVYQVAFSYLDTAYAATLTVVLLTLLAAFAVLQ
                     FRMIERRVHYR"
     gene            complement(4311..5585)
                     /locus_tag="VIG70_13720"
     CDS             complement(4311..5585)
                     /locus_tag="VIG70_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251659.1"
                     /translation="MKRLLTLVFTALSACMAHGQGKTELTFYYPIAVGGPLTKVIDGY
                     AAGFEKEHPNVKVNAVYAGNYDDTRIRALAAHKAGQPAQLSVLFSIDLYELLEQDLIV
                     PFDEVATSAEDKAWLKSFYPALMSNGTYKGKVYGIPFQRSTIVLYWNKDAFKEAGLDP
                     EKPPASWNEMRTMAAKLVKKDASGNVSRWGVMVPSTGYPYWMFQAFSRQNGQDLMNPE
                     GNRTNFTHPDVINALQYWTDLGRKHRVMPAGTVEWGTLRQNFVEGKTAMMWHTTGNLT
                     AVKDGAKFAFGVTMLPASKQRGSPTGGGNWYLFKKSTPAERKAAFEFIKWITAPERAA
                     DWSIATGYVAVSPAAYDTPKLKTYVSGFPAAAVARDQFKFATPELSTFQTGRVRKSLD
                     DAIQAALTGSKTPAEALTAAQTDAERLLKPYR"
     gene            complement(5582..6577)
                     /locus_tag="VIG70_13725"
     CDS             complement(5582..6577)
                     /locus_tag="VIG70_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251660.1"
                     /translation="MIVLEGVAKRFGAVKAVDGVSFRADKGKFVVLLGPSGCGKSTLL
                     RLIAGLEDVSEGKILIEGRDVTALDPTKRRISMVFQSYALFPHLTVAENIIFGLKVRR
                     TAPADRERRLQDVAELVGLSQRLHHKPQQLSGGQRQRVALARAIVSQNPICLMDEPLS
                     NLDAQLRHDMRVEIRALQRRLGMTVVYVTHDQVEAMSMADHVVLLREGRIEQEGSPAE
                     LYSRPASTFAARFIGTPAMNLVPFQALQPGAAAGLTAGIRPEHIKIIPGGAGLAATVL
                     SAEYHGADTIVTAQVNGSSLLVRAPGQVELAAGSQVKLGWEPAAVHIFDSEGRKQ"
     gene            6615..>7485
                     /locus_tag="VIG70_13730"
     CDS             6615..>7485
                     /locus_tag="VIG70_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY8251661.1"
                     /translation="MALKRPKGPAGTLVVLEHTSEILKGNALGDPHVRKLGVWLPPQY
                     DGFRGRRRFPVLYDLVGFTGSGLAHANWKPFGDNVPERAARLIHEQKMGPAIIVFPDC
                     FTALGGNQYVNSSAIGDYADYLTREIIPFVDREFRTLADRESRGCFGKSSGGYGAILH
                     GMKYAKYWGAVASHSGDAYFDFVYWHDWPNTLDELAKHRVPKRKSGAYDARREANRKG
                     LAQGLDDGRIKRFLDHVWKKEKLSGKEGHAIMNLCMAATYDPDPRAPLGFRIPFNAES
                     GEVIEERWRKWRRH"
BASE COUNT         1268 a         2352 c         2640 g         1225 t
ORIGIN      
        1 atggcctgcc tcggctgcga caaggacggc gagctcaagc gtccagccgc gcacacgctg
       61 ctctcccgcg cctggtccgt cgccgaggcg tacttcagcg cggacgctgc cgcggctcac
      121 actgtagaac ccgagcgcat cgaacctggc gctactttcc agcgcctcgc gcgcggctgg
      181 cgcatcagtc tgcctccgca agccgtgcgc tggacgacgc ctccggaggg tcgcgacgag
      241 caggagatcg agttctcgtg ggcgggcgaa actgcgcggc acgtcggcac agtggtccat
      301 cactggttgc agtgcattgc cgacgacgcc atgcgcggct gggacgcgag gcgcgtggag
      361 agcctgcgtc cgctgttcgc gcgcgagctc gagtgccgcg gcatccagcg ttcgcgagcg
      421 aaggaggtag ccggcctcgt tgccgacgcg ctgaagaact ctctcgccga cgatcgtggc
      481 cgctggctgc taggcccgca tccgcaagcc cgcagcgagc accgtttgcg tctgcgaagt
      541 accgtcggcg taagaaccta cgtgctcgac cgcctattcc gcgatacggc cggcgagcaa
      601 tggattgtcg atttcaagac cagccggcac gagggcggcg gggtagagga gtttctcgac
      661 gagcagcgca agcggtacga accgcaactc aggagctacg cggccgcttt cgacaacgcg
      721 cgcttaggtc tttactttcc actgcttcgc ggctggcgag aatggttgga tccaagcacg
      781 ttggattgtt gaccttgcga gcagcggatc cattgggtcc atcgttgact tagcggcctt
      841 atggcaatgt cagctcgctc agcgttggtc ctactcgctg aggacgatgc ccgacttcgt
      901 gatcacctcg gcgaggcgct ggcgctcgcg cgccatgaac gcttcgaagg cctgcggcga
      961 gtcgcccgcg ggcaccgcgc ccatcttcag cacgcgctcg cgcacgtcga cgagcgccat
     1021 cgccctgacg atttcctcgt tcgcgcggcg caggaccggt tgcggcgtgg cggcgggcgc
     1081 cacgacgccc caggactgcg tgatctcgta gccgggcacg gtgtcggcga ttggcggcag
     1141 ctcgggcagg ctcgggttgc gctcgatgcc ggtcgtggcg agcgcacgca cgcggccggc
     1201 gttcatgtgc gcgcccgcgg aaatcggcga ggtgatgatg acgtgggcat tgccggcgat
     1261 cacgtccagc agcccgggcg cggcgccctt gtaaggcacg tgcgtcatct tgatccccgc
     1321 aagcaggttg agcatctcgc ccgcaaggtg cgccggcgtg ccgatcccgg ccgaggcgta
     1381 gttgagggct ccgggcttgc tcttcgagag ctgtaccagc tcggcgaccg accttgcagg
     1441 cagcgaggtg ttgattacca gcacgttcga ggtggtggcg agcctcgaga ttggcgcgaa
     1501 gtcgcgcacc gggtcgtacg gcaggtcttt cttcaggctc ggcaggatcg cccagatcgc
     1561 ggtggtcgac agcaggagcg tgtatccgtc cgcggtcgcc ttggcggcga ggtcggcgcc
     1621 gagcgtgccg cccgccccgg gacggttgtc gacgatgacc tgctggccga aaccctccgc
     1681 gagcttcgcg gaaaggatgc gcgcgagctg gtcgggcgca ccgccggccg cgtcggccac
     1741 gatgaggcgc accggcttcg cgggccatgt ctgcgcggca gcgttggcgg cgaagagcag
     1801 cagcaggagc gcacctcgaa gcatcgtctt cattcgtttc tcccaagttc atgtcgggcg
     1861 cctgcccgtg cttgcctcga tgatatcgcg cgcaccgtag gggaatcgct tgaagttaga
     1921 acatgcgctc ccatcgctct acttgatacc ggcctgcatg aacgactgca tgaactgccg
     1981 ctgaaatagc aggaacgcga ccagcaacgg ggcgacggca atcaacgtac cggcgctgat
     2041 gacggaccaa tcgacgcccg actccggcgc gccgaagatc gcgaggccga cggtgagggg
     2101 tcgggtgttc acggagttgg tcaccacgag cggccagagg aagttgttcc agtggtagct
     2161 caccgagacc agggcgtagg cgagataagt cgggcgagct agcgggacgt agacgcgcca
     2221 cagaacgccg agccatgagc agccctccac gcgcgcggct tcctcgagct cgcgcgggat
     2281 ggtcttgaag gtctggcgca ggaggaagat gccgaacgcg ctcgccatgt agggaaggcc
     2341 gatgcccagc agggtgtcga tgaggccgag gcggctcatg ctgcggtagt tctcgacgat
     2401 cagcacctcc ggcatgatca tcagctgcag gagcacgagc gcgaaggcga tgtcgcggcc
     2461 ggcgaagtcg aagcgagcga aagcgaagcc ggcgagggtg cagaggacga gctgcgcacc
     2521 gaggatcatg gtgacgagca tcacggtgtt caggaagtgc cgcgggaagg gcgcgtagcc
     2581 ccaggctttc tcgaagttgg cgagggtgag cggcgcgagc ggctcgaagg tggtggcgaa
     2641 ctcgggcggg tggaaggcgg cccagaaggc atagaggagc ggtgagaccc acaggatcgc
     2701 gagcagccag gcggcgattt tttccacgtg ggtcattgag tcccgccccg cgacgggtac
     2761 agggtccgga gctgccgccg ttcttcttcg gtaatcggaa agatgtgggg ccgctcagag
     2821 gcgtaacccg gcggccggca cggcgccggg cggccgcaca tcagcattgc cgggtcgggg
     2881 ttgtgtccga ggccgatcgc atggccgact tcgtgggcga tgacgttgcg cgcgacgttc
     2941 ggcaggttca gcggccatac gtccatgccg cgaataccga ccacgatcct gccgtcaggg
     3001 tcgaaaggcc cggcaaagga cacgaatccg gagtgcgcga gcatgatggt caggtccccg
     3061 ggcaggttgc gcagcgcctc cggcacctgg aacggcctgc ggccaacgcc gactatggcg
     3121 ttgctcatct cctgcagtgc tggttccggg atcggcagcg cgctgcgcgt cgccgcggga
     3181 agcctaaagg cgctgccggt atcttcgagc aagcggttcc agtaggcgat ggcctcgtcg
     3241 acgagggcga tgcgcgggtc gccttctgtg gcgacgacca cgatagtcgg cgtcttgcgc
     3301 cagggcatgc cgaactgcgc ctgggcgctt gcggccgcaa tcgcgccggc gatgagggcg
     3361 aggatcacgg cgctgtgcgt ggctcgcatg tcagcggtag tgcacgcgcc gctcgatcat
     3421 gcggaattgc agcacggcaa aagcggccaa gagagttagc agaacgaccg tcaaagtcgc
     3481 ggcgtaggcg gtgtcgaggt agctgaaggc gacctggtag acgtagtaca ggagcaggtt
     3541 cgaggcgttg tccgggccgc ccttggtcaa gatgaagagg tggtcgacca gcttgaagga
     3601 gttgatggtg gcgttcacca gcacgaagag cgtggtgggc atgaggagcg ggaaggtgac
     3661 gcgccagaag aagtagccgc gcgaggcgcc ctcgatgcgc gccgcctcct cgagctcggg
     3721 cggcagcgac tgcagcgcgg cgaggtagaa gatcatgaag aagcccgctt ccttccagat
     3781 cgtcatggcg atgacgcagc ccatcacggt atcggggttg ccgagccagt tgtggctggg
     3841 cacgccgaag agggcggtga ccttgtcgag caggccgatc tgcggggtgt agaagtagag
     3901 ccacaggttc gcgaccgcga tcatcggcag cacggtgggc gtgaagtagg agagccgcac
     3961 cagcgcgcgg cctgggaggc gcgcgttgac ccacagcgcc atcaatagcg cgagcgcgat
     4021 cgaggtgggg atggtgccga gcgcgaacca gaaattgttc cgcagcacct gccagaacac
     4081 cgggtcgtcg gcgagcgcgg cgtagttgtc caggccgacg aagcgcgagg ggcgcagcgc
     4141 cgtgccggcc gagaagaagc tgtcgacgag ggtggcggcg gccgggtagt gcgtgaagcc
     4201 gacgagcagg atggctgctg gagcgagcag cagccatccg tacacgtgac gcacttaaac
     4261 cccccgctcg cgaaaaaccc gctcgcgtcc ccccttatca ggggggcaaa tcatctgtac
     4321 ggcttcagca gcctctcggc gtccgtctgg gccgcggtca gcgcctcggc cggggtcttc
     4381 gagccggtga gcgcggcctg gatcgcgtcg tcgaggctct tcctcacgcg gccggtctgg
     4441 aaggtggaga gctcgggcgt cgcgaacttg aactggtcgc gcgccaccgc ggcggccggg
     4501 aagccggaga cgtaggtctt gagcttcggc gtgtcgtaag cggcggggct gaccgcgacg
     4561 tagccggtgg caatgctcca gtcggccgcg cgctcgggcg cggtgatcca cttgatgaac
     4621 tcgaacgccg ccttgcgctc ggcgggcgtc gacttcttga agagatacca gttgccgccg
     4681 ccggtgggcg agccgcgctg cttggaggcg ggaagcatcg tcacgccgaa ggcgaacttg
     4741 gcgccgtcct tcaccgcggt aaggttgccg gtggtgtgcc acatcatcgc cgtcttgccc
     4801 tcgacgaagt tctgccggag cgtcccccac tcgacggtgc ccgcgggcat cacgcggtgc
     4861 ttgcggccga ggtcggtcca gtactgcagg gcgttgatca catccgggtg cgtgaagttg
     4921 gtgcggttgc cttccgggtt catcaggtcc tggccgttct ggcgcgagaa cgcctggaac
     4981 atccagtagg gatagccggt cgagggcacc atcacgcccc agcgcgagac gttgcccgag
     5041 gcgtccttct tcaccagctt ggccgccatc gtgcgcatct cgttccagct cgccggcggc
     5101 ttctccgggt cgaggccggc ttccttgaac gcgtccttgt tccagtacag cacgatggtc
     5161 gagcgctgga acgggatgcc gtacaccttg cccttgtagg tgccgttgga catcagcgcc
     5221 gggtagaagc tcttaagcca cgccttgtcc tcggcgctgg tcgcgacctc gtcgaagggc
     5281 acgatcaggt cctgctcgag caattcatac aagtcgatgg agaacagcac cgagagctgc
     5341 gccggctgcc cggccttgtg cgcggcgagc gcgcggatgc gcgtgtcgtc gtagttgccg
     5401 gcgtacaccg cgtttacctt gacgttcggg tgctccttct cgaacccggc ggcgtagccg
     5461 tcgatcacct tggtgagcgg cccgcccacc gcgatcgggt agtagaaagt gagttccgtc
     5521 ttgccctggc cgtgggccat gcaggcggag agcgcggtaa agaccagtgt gagaagtcgc
     5581 ttcattgttt cctcccctcg gaatcgaaaa tatgcaccgc cgcgggctcc cagccaagct
     5641 ttacttgcga tccggccgcg agctcgacct ggccgggcgc gcgcacgagg agcgaggagc
     5701 cgttgacctg agcggtgacg atggtgtcgg cgccatggta ttcggcgctg agaaccgttg
     5761 cggccaaacc cgctccccct ggaatgatct ttatgtgctc agggcggatg ccggcggtca
     5821 ggcccgcggc agcgccgggt tgcagggcct ggaaaggaac cagattcatc gccggcgtgc
     5881 cgatgaagcg cgcggcgaag gtgctcgccg ggcgcgaata gagctcggcg ggcgagcctt
     5941 cctgctcgat gcggccctcg cgcagcagca ccacgtggtc ggccatgctc atcgcctcga
     6001 cctggtcgtg ggtgacgtag accaccgtca tgccgaggcg ccgctgcagc gcgcggatct
     6061 cgacccgcat gtcgtgccga agctgcgcgt cgaggttgga gagcggctcg tccatcaggc
     6121 agatcgggtt ctgcgacacg atcgcgcgcg cgagcgccac gcgctggcgc tggccgccgg
     6181 aaagctgttg cggtttgtga tgaagtcttt gcgaaagacc cacgagctct gcgacgtctt
     6241 gcagacgccg ctcgcgatcc gcgggcgccg tgcggcggac cttgagaccg aaaatgatgt
     6301 tttccgccac ggtgaggtgc ggaaagagcg cgtaggactg gaacaccatc gagatgcggc
     6361 gcttcgtcgg gtcgagcgcg gtgacgtcgc gcccctcgat aagaatcttc ccttcggaaa
     6421 cgtcttccag gccggcgatg agccgaagga gggtcgactt gccgcagccc gacggcccga
     6481 gcagcaccac gaacttcccc ttgtcggcgc ggaagctcac gccgtcgacc gccttaacgg
     6541 cgccgaagcg cttggctacg ccctccagga cgatcatggg cgcgatatta agcttcccgg
     6601 ctagactcgc ccgcatggct ctgaaacgcc ccaagggccc cgccggcacg ctggtcgtgc
     6661 tcgagcacac ctctgagatc ctcaagggaa acgcgctcgg cgatccgcat gtgcgaaagc
     6721 tcggggtctg gctgccgccg cagtacgacg ggttccgggg caggcgacgc ttcccggtgc
     6781 tctacgacct cgtcggcttc accggctcgg gcctcgcgca cgcgaactgg aagccgttcg
     6841 gcgacaacgt ccccgagcgc gcggcgcggc tgatccacga gcagaagatg gggccggcga
     6901 tcatcgtgtt cccggactgc ttcaccgcgc tcggcggcaa ccagtacgtg aactcctcgg
     6961 cgatcggcga ttacgcggac tacctgacgc gcgagatcat tccgttcgtc gaccgcgagt
     7021 tccgcaccct cgcagatagg gagagtcgcg gctgcttcgg caagtcctcg ggcgggtacg
     7081 gcgcgatcct ccacggcatg aagtacgcga agtactgggg cgcggtcgcc agccattcgg
     7141 gcgacgcgta cttcgacttc gtctactggc acgactggcc gaacacgctc gacgagctcg
     7201 ccaagcaccg cgtgcccaag cgcaagtctg gcgcctacga cgcgcgccgc gaggcgaacc
     7261 gcaagggcct cgcccagggc ctcgacgacg ggcgcatcaa gcgcttcctc gaccacgtgt
     7321 ggaagaagga gaagctctcg gggaaggaag gccacgcgat catgaatctc tgcatggccg
     7381 cgacctacga tcccgacccg cgcgcgccgc tcggcttccg catcccgttc aacgccgagt
     7441 cgggcgaggt gatcgaggag cgctggcgca agtggcggcg ccacg
//
LOCUS       DATBSZ010000219         4002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1131862, whole genome shotgun sequence.
ACCESSION   DATBSZ010000219 DATBSZ010000000
VERSION     DATBSZ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131862"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..486)
                     /locus_tag="VIG70_13735"
     CDS             complement(37..486)
                     /locus_tag="VIG70_13735"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8251662.1"
                     /translation="MNEQTVALTRVFEAPRERVFACWTRAEHLQHWFGPKGFTIHSCE
                     ADARPGGKFKLCLRAPDGKDYWVRGEYREVAAPEHLLIACVADDHTGVERLHETIDVT
                     FTESRGRTTVALVAKASGPSDEAAAMLKGMDKGWAQTVARLDAHLRK"
     gene            complement(483..962)
                     /locus_tag="VIG70_13740"
     CDS             complement(483..962)
                     /locus_tag="VIG70_13740"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8251663.1"
                     /translation="MKPFETSRVFDAPRAKVWQAWTEPARLKQWWGPKGFKVHTCKLD
                     LRPGGTFLYGMKAPDGSDMWGKFVYREIKAPEKLVFVVSFSDPQSGVTRHPMSPGWPQ
                     YILSTVEFSEVSGKTRVTVSWTPHEATEAERKVFEEGRESMKQGWTGTFEQLEAYLK"
     gene            complement(959..1315)
                     /locus_tag="VIG70_13745"
     CDS             complement(959..1315)
                     /locus_tag="VIG70_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEY8251664.1"
                     /translation="MYVDGFVLPVPKRNLKAYRAMATKAGKVWREHGALDYKECIADD
                     VKKGKHTSFPQAVKLKPGEVVWFSWILYKNRKQRDSVNKKVMKDKRLASMMDPKKMPF
                     DGKRFFWGGFKVVVDL"
     gene            complement(1433..2641)
                     /locus_tag="VIG70_13750"
     CDS             complement(1433..2641)
                     /locus_tag="VIG70_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8251665.1"
                     /translation="MSERRYGWVMVALGAFMTCVAVGAMFSLAVFLRPITESTGWSHT
                     AVSAAMTLNFLAMGAAGFGWGALSDRIGPRPVVLAGAVLLGLGLFLASSAQSALAFQV
                     SFGLLVGIAVGSFFSPMIAAVMGWITERRGLAVALVSVGVGVAPMTMSPFAAWLITQM
                     DWRAALTVFAVLVWATLVPAALFARRPPAAPAAEQTAGEGPQMSLRDAFVSAPFVILA
                     LTFFACCAAHAGPIFHTVTYAMTCGIAPLAAVTVYSVEGLAGLGGRLALGVLADRLGV
                     KRVLIAGLLIQAVAAFAFIFASRLGEFYAVAAMFGFAYGGVMPLYAVLAREYFGQRIL
                     GAVFGAAAMVSSLGMALGPAIGGWIFDTFGRYTWMYVYSAAIGLGAVAIALAFPPFAK
                     PALKLQGAQA"
     gene            complement(2638..3084)
                     /locus_tag="VIG70_13755"
     CDS             complement(2638..3084)
                     /locus_tag="VIG70_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8251666.1"
                     /translation="MEPRLAEKPSLTLSRRYPVAPEKVWRAWTDAQAVRKWWGPGPGE
                     PVSVAELDVRVGGRFRIVFGGADGKAHECAGVYREVVPNRKLVFTWSWPNTTPERVSV
                     VTIEFKKIDEGTELVFKHEQLFDEKVRDDHKRGWSGSLDKLAAYLA"
     gene            complement(2982..3422)
                     /locus_tag="VIG70_13760"
     CDS             complement(2982..3422)
                     /locus_tag="VIG70_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8251667.1"
                     /translation="MVKYQDPALDAVFAALADPTRRGILESLSEGELAVSELAAPHDM
                     SLPGFMKHLRVLEDAGLIARNKEGRVVSCELSAAPMKAAAAWMSRYEKFWTEKLDSLA
                     RYLYQQEELQTWNRDWPRSPRLPSAAATRSRRRKSGARGPTRKR"
     gene            3490..3828
                     /locus_tag="VIG70_13765"
     CDS             3490..3828
                     /locus_tag="VIG70_13765"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY8251668.1"
                     /translation="MKLTGMSLAERVRKALSGRRAITEKKMFGGTCFLLRSHMLCGTG
                     KQGFMFRIGRAQHVKALARGARPVVMGGREFEGFVWVDPGSCDARALKRWIVLAEDYV
                     GALPPKRKSR"
BASE COUNT          669 a         1362 c         1317 g          654 t
ORIGIN      
        1 ctgccgcgac cgcggccagt atccatttca gcatgatcac ttccttaagt gtgcgtccag
       61 gcgcgcgacg gtctgcgccc agcctttgtc cattcccttg agcatcgcgg cggcctcgtc
      121 gctcggcccg ctcgccttgg cgaccagcgc gaccgtcgtg cggccgcgcg actcggtaaa
      181 cgtcacgtcg atcgtctcgt gcagccgttc gacgcctgtg tggtcgtcgg cgacgcacgc
      241 gatgaggaga tgctcgggtg cggccacctc gcggtattcg ccgcgcaccc agtagtcctt
      301 gccgtcgggt gcgcgcaagc acagcttgaa cttgccgccc ggccgcgcgt ccgcctcgca
      361 ggaatggatc gtgaagcctt tcggcccgaa ccagtgctgc aggtgctcgg cgcgcgtcca
      421 gcacgcgaac acccgctcgc gaggcgcttc gaagacgcgc gtcagggcga cggtctgctc
      481 gttcatttca agtaggcctc gagctgctcg aaggtcccgg tccagccctg cttcatgctt
      541 tcgcggcctt cctcgaacac cttgcgctcg gcctcggtcg cctcgtgcgg ggtccacgag
      601 accgtgaccc tggtttttcc ggacacttcg gaaaattcca ccgtcgacag aatgtactgc
      661 ggccaccccg gcgacatcgg atgccgggta acgccgctct gcgggtcgga gaaggagacg
      721 acgaacacca gcttctccgg cgccttgatc tcccggtaga cgaacttgcc ccacatgtcg
      781 ctgccgtccg gcgccttcat gccgtagagg aacgtgccgc cggggcgcag gtcgagcttg
      841 caggtgtgga ctttgaaccc cttcggtccc caccactgct tcaggcgcgc gggctcggtc
      901 caggcctgcc agaccttggc gcgcggcgcg tcgaagacgc ggctggtctc gaaaggcttc
      961 acaggtccac gaccaccttg aagccgcccc agaagaagcg cttgccgtcg aacggcatct
     1021 tcttcgggtc catcatggac gcgaggcgct tgtccttcat caccttcttg ttcacgctgt
     1081 cgcgctgctt gcggttcttg tacaggatcc aggagaacca caccacctcg ccgggcttga
     1141 gcttcaccgc ctgcgggaac gaggtgtgct ttcccttctt cacgtcgtcg gcgatgcact
     1201 ccttgtagtc gagcgcgccg tgctcgcgcc acaccttgcc ggccttggtg gccatggcgc
     1261 ggtaggcttt caggttccgc ttcggtaccg gcaatacgaa tccgtctaca tacatggaat
     1321 cctccttcgg gttaatcgga tgagatccgg aacttgtccg gatagaaggc gagccgttcc
     1381 cggatcgccc gcatttcgtc gtagggcgcc acagtcctct ttcccgccgg attcatgctt
     1441 gcgccccctg caacttgagg gcgggtttcg cgaacggcgg gaaggcgagc gcgatcgcga
     1501 ccgcgccgag accgatcgcg gccgagtaga cgtacatcca cgtgtagcgg ccgaacgtgt
     1561 cgaagatcca gccgccgatt gccggcccga gcgccatgcc gaggctcgac accatggcgg
     1621 ccgcgccgaa caccgcaccg aggatgcgct gcccgaagta ttcgcgcgcc aggaccgcgt
     1681 agagcggcat caccccgccg taggcaaagc cgaacatggc ggcgacggcg tagaactcgc
     1741 cgagccgcga ggcgaagatg aacgcgaacg cagccacggc ctggatcagc agtccggcga
     1801 tcagcacgcg cttcacgccc aggcggtcgg cgagcacgcc gagcgcgagg cggccgccga
     1861 gcccggcgag gccttcgacg ctgtagaccg tgaccgcggc gaggggcgcg atgccgcagg
     1921 tcatcgcgta ggtcaccgtg tggaagatcg ggccggcgtg cgccgcgcag caggcgaaga
     1981 aggtgagcgc gaggatgacg aacggcgccg agacgaacgc gtcgcgcagg ctcatctgcg
     2041 ggccttcgcc cgcggtctgc tccgccgccg gcgccgcggg cggtcgacgc gcgaagagcg
     2101 cggccgggac aagggtcgcc cacaccagca cggcgaagac cgttagagcg gcacgccagt
     2161 ccatctgggt aatgagccag gcggcgaacg gcgacatcgt catcggcgcg acgccgaccc
     2221 cgaccgaaac cagcgccacg gcgaggccgc ggcgctcggt gatccacccc atcaccgcag
     2281 cgatcatcgg cgagaagaag ctgcccacgg cgatgccgac cagcaagccg aaggaaactt
     2341 gaaacgcgag cgcgctctgc gcgctgctcg cgaggaacag gcccagtcca aggagcactg
     2401 cgcccgccag caccaccggg cgcgggccga tgcggtcgct gagcgctccc cagccgaagc
     2461 cggccgcgcc catcgccagg aagttgagcg tcatggcggc cgacacggcg gtgtgcgacc
     2521 agccggtcga ctcggtgatc ggccgcagga ataccgcgag cgagaacatg gcgccgacgg
     2581 cgacgcacgt catgaacgcg ccgagcgcca ccatcaccca gccgtagcgc cgctcgctca
     2641 tgcgagatag gccgcgagct tgtcgagcga gccgctccag ccgcgcttgt ggtcgtcgcg
     2701 caccttctcg tcgaagagct gctcgtgctt gaacacgagc tcggttccct catcaatctt
     2761 tttgaattct atggtcacga ctgagacacg ctcgggcgtg gtgttgggcc agctccaggt
     2821 gaacacgagc ttgcggttcg gcaccacttc ccggtagacg ccggcgcact cgtgcgcctt
     2881 gccgtccgcg ccgccgaaca cgatgcggaa gcgcccgccg acgcgcacat cgagctcggc
     2941 cactgacacc ggctcgcccg ggccgggccc ccaccacttc cttaccgctt gcgcgtcggt
     3001 ccacgcgcgc cagactttct ccggcgcgac cgggtagcgg cggctgaggg taagcgaggg
     3061 cttctcggcc agtctcggtt ccatgtctgc aattcctcct gctgatagag atagcgggcg
     3121 agcgagtcga gcttctcggt ccagaacttc tcgtagcgcg acatccaagc cgccgcggcc
     3181 ttcatcggcg cggcgctcag ctcgcagctc acgacgcgcc cttccttgtt gcgcgcgatg
     3241 agcccggcgt cctcgagcac gcgcaggtgc ttcatgaacc ccggcagcga catgtcgtgc
     3301 ggcgccgcca gctcgctgac cgccagctcg ccctcgctca gggactcgag gatgccgcgc
     3361 cgggtcgggt ccgccagcgc cgcgaacacg gcatcgagag cggggtcttg atacttaacc
     3421 atcaggttta gtatcattca acgcgttgac gattgtcaag accaggaacc ggccgaagtt
     3481 acagatgaca tgaagctgac gggcatgtcg cttgcagaac gcgtgcgcaa ggcgctctcc
     3541 ggcaggcgcg cgattaccga gaagaagatg ttcggcggga cctgcttcct cctacgcagc
     3601 cacatgctct gcggcacggg gaagcagggc ttcatgttcc gcattggcag ggcacagcac
     3661 gtgaaggcgt tggcgcgcgg cgcgcggccg gtggtgatgg gcggccggga gttcgagggg
     3721 ttcgtctggg tcgatcccgg gagctgcgac gcccgggccc tgaagcgatg gatcgtactc
     3781 gccgaagatt acgtcggtgc gttgccgccg aagaggaaat cgcgatgaag atcggggacg
     3841 gagccctaac tccagcgacg cgcagctttc ggagttaggg ctccgtcccc gaattcagta
     3901 gccggcgtcg aagtcgacca ggttgagcgg cggctcgccg cggcgcaggc gctcgacgtt
     3961 ctcgacgatc ttggggatcg cggtgcgcgg ctcggtgagc gc
//
LOCUS       DATBSZ010000220         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1200794, whole genome shotgun sequence.
ACCESSION   DATBSZ010000220 DATBSZ010000000
VERSION     DATBSZ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200794"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VIG70_13770"
     CDS             <1..196
                     /locus_tag="VIG70_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251669.1"
                     /translation="ITRIKELKERSGLTVLMAEQNFHQAIRIADRGYIIVHGEIVFEG
                     GVRALEQNELVKNYYLGAAV"
     gene            193..1131
                     /locus_tag="VIG70_13775"
     CDS             193..1131
                     /locus_tag="VIG70_13775"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251670.1"
                     /translation="MSVRELPIGFYGAVMGLAGLGLTARAAAPLFPGVMRAPAYFTEL
                     WVGLGALAFILLLAGYLAKLVRYPRAVKDEFTDPVQLGFCSALPVGMSLVAGGVGAYS
                     PFIGGILWWAAFVLLLAFQGWALARLLGGGIELAQVNAGWLIVFGGGFVLPGPGIALG
                     YVEASRFSFGFSTVAASLLMPLLLYRAFAAPPLPAVLRPSWFILLAPPTLIYVNGLAL
                     YPDAAFLEILYPFSLVLAAALLLYARHLRRWPFGVPWWAFTFPLDALAYAATRYALDR
                     QTELWRAVAGATLLLATLCVAMVLWRTLRSAASRRA"
     gene            1742..2638
                     /locus_tag="VIG70_13780"
     CDS             1742..2638
                     /locus_tag="VIG70_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEY8251671.1"
                     /translation="MKAHRQVWLLVAGAVAPIVVFASLAALLLVQHERQTLERDAIGR
                     ARAAMTAVDAHLQGSIASLETLAASKSLEAGDLAAFHEESQRVLRTQPGWMNIGLASA
                     AKVQLSDAVYVFGKPAPFSANEDSFDAVLRGAKAAMGNVMAGSAVRSPSARIRVPVIV
                     GGEVRYVVSAPLNPKQFAELLQAQRLPEDWVIGLVDREKRVVARIPAVAPGTPASDNF
                     REAIEREPEGWFYGRTLEGRATYTSYVTSPLSGWVLGIAIPAVTVESEVRRIFAILGA
                     GALCACAVAALLAWLIAGRLSR"
     gene            2635..>3307
                     /locus_tag="VIG70_13785"
     CDS             2635..>3307
                     /locus_tag="VIG70_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEY8251672.1"
                     /translation="MRLHPQLSLLVVGTLAPVLMLAVAAGVLLVRHERNTMQEEAIGR
                     TRSAMSAVDALINGHLTTLQALAASRALETGNIRAFYEETRRVLRAQPEWLNVGLASA
                     DGIQLFDAVRPFGTETPLTGDLASYRLAVERGMPVIGDVDEGIVVPDPSVRIRLPVVL
                     KGSVRYVLTLPLKPDAFSDVLRAQRLPESWVIALVDRNRRFIARLPPMAPGRAVSESF
                     RAAIER"
BASE COUNT          443 a         1095 c         1161 g          608 t
ORIGIN      
        1 catcacgcgg atcaaggagc tgaaggaacg cagcgggctt accgtgctga tggcggagca
       61 gaacttccac caggcgatcc gcatcgccga ccgcggctac atcatcgtgc acggcgagat
      121 cgtgttcgag ggcggcgtgc gggcgctcga gcagaacgag ctggtgaaga actactacct
      181 cggcgcggcc gtttgagcgt ccgagagctt ccgatcggat tctatggggc ggtgatgggc
      241 ctggccggat tggggctcac tgcgcgcgcc gcggcgccgc tgtttcccgg cgtgatgcgc
      301 gcgccggcgt atttcaccga gctatgggtc ggtttgggcg ctctggcttt catccttctc
      361 ctggctggtt acctcgcaaa gctcgtgcgc tatcccaggg cagtgaagga cgagttcacg
      421 gacccggtcc agctcggctt ctgcagcgcg ctgccggtcg ggatgagcct ggtcgcgggc
      481 ggggtcggtg cctattcgcc gttcatcgga ggaatcttgt ggtgggcagc ctttgtgctg
      541 ctactcgcct tccagggatg ggcgctcgcg cgcctgctcg gcggcggcat cgagctcgcc
      601 caggtgaacg ccggctggct gatcgtcttc ggcggcggct tcgtgctgcc ggggccgggc
      661 atcgcgctcg gctacgtgga ggcttcgcgc ttcagcttcg gattctcgac ggtcgccgcg
      721 tcgctgctca tgccgctcct tctgtaccgc gcgttcgccg cgccgccgct gcccgcggtg
      781 ctgcggccga gctggttcat cctgctcgcg ccgccgacgc tcatctacgt caacggactc
      841 gcgctctacc cggacgccgc tttcctggag atcctctacc cattctccct ggtgctggcc
      901 gccgcgctgc tcctctacgc gcgccatctg aggcgttggc cattcggcgt gccgtggtgg
      961 gcgtttacct tcccgctcga cgcgcttgcc tacgccgcga cgcgctatgc gctcgaccgc
     1021 cagaccgagc tgtggagagc ggtcgccggc gcgacgctgc tactggcgac gctctgcgtg
     1081 gcgatggtgc tttggcgaac tcttcgcagc gctgcatcgc ggcgggcgtg aactcggcct
     1141 gcggctggtt gccgccgatg atgaacatca gcaggtgcga tttagtgggc tggtcgtagg
     1201 tcacgttcat gaagcgccgc gcgacgccga cgggaaacga gatcacgtcg tagggctcga
     1261 ggtcgacgtg ctccatcgcc gcgccctcgt tccactcgca gcgccagcgg ccggtcatgg
     1321 cgatgaaggt ctcgttggtg tcgtggttgt gcatcagggg ccccgtgccg ggcttcgcct
     1381 tgcaatagcc gaggttgaag ccttcggcga tcgagatcgc gggcatggcc gaggcattgg
     1441 cgcccaccgg cgaatcaccg gtttccggcg gttggaagcc gatcaccgca tagagcttgc
     1501 ggcggcactc gggaagcttg ctgtccggca gcccgccgtc ggaccctttc agttgcttga
     1561 agcgtgcgac ccgctgcagc atctcgtcgc gggaccagtt gcggactgct ttccagtcgt
     1621 ccatgccttc ctcctgccgc gattatgcgg cacggcgggc gcaggattct aggcggccgc
     1681 gccgcggtgg gcggcgattc acggtctatc cgctgatcgg atcaggctat gatcgccacg
     1741 catgaaagcg catcggcagg tatggctgct cgtcgctggc gcggtcgcgc ccatcgtcgt
     1801 ttttgcgtcg ctcgcggccc ttctactggt ccagcacgag cgccagaccc tggagcggga
     1861 cgcgatcgga cgcgcgcgcg ctgccatgac tgctgtggac gcccacctgc aaggatcgat
     1921 cgcttccctg gagacgctcg ctgcctccaa gagtctcgaa gcgggcgacc tcgcggcgtt
     1981 tcacgaggaa tcgcaacgcg ttctgcgtac ccagcccggc tggatgaaca tcggcctcgc
     2041 gtcggccgcg aaggtacagc tttcggacgc ggtttatgtc ttcggcaagc cggcgccgtt
     2101 ctctgccaac gaggactcct tcgatgccgt gttgcgcggc gccaaagccg ccatggggaa
     2161 cgtcatggcc ggcagcgcgg tccgaagccc gagcgcgcga atacgcgttc cggtgatcgt
     2221 gggcggcgag gtacggtacg tggtgtcggc gccgctcaac ccgaagcagt tcgccgagtt
     2281 gctgcaggcc cagcggctgc ccgaggactg ggtgatcggt ctggtcgacc gcgaaaagcg
     2341 cgtcgtcgcc cgcatccccg cggttgcgcc cggcacgccg gcatcggaca acttccgcga
     2401 agcgatcgag cgcgagcccg aggggtggtt ctacggccgc accttggaag ggcgagctac
     2461 ctacacctcc tacgtgacct cgccattgag cggctgggtg ctcgggatcg cgatcccggc
     2521 ggtcaccgtg gagtccgagg tgcggcgcat cttcgcgatc ctgggcgcgg gcgcgctctg
     2581 tgcctgcgcc gtggccgcgc tgctcgcatg gctgatcgcc ggacggctat cccgatgagg
     2641 ctgcatccgc agttgtcgct gctggtcgtc ggcacgctcg cgccggtgct gatgctggcg
     2701 gtcgcagcgg gcgtgcttct ggtgcgccac gagcgcaaca cgatgcagga ggaggcgatc
     2761 ggacgcacgc gctcggcgat gagcgcggtg gacgcgctga tcaacggaca cctgaccacg
     2821 ctccaggcgc tcgcggcgtc gcgggcgctg gagaccggaa acatccgcgc cttctacgag
     2881 gagacccggc gcgtgctgcg cgcccagccc gagtggctga acgtcggcct cgcctcggcg
     2941 gacgggatcc agttgttcga cgcggtgcgg cccttcggta ccgagacgcc gctcaccggc
     3001 gatctggcga gctaccggct ggccgtcgaa aggggcatgc cggtgatcgg cgacgtcgac
     3061 gaagggattg tggtgccgga cccgtcggtg cgcatccgcc tgccggtagt gctgaagggg
     3121 agcgtgcgct acgtcctcac cctgcccctg aagccggacg ccttctccga cgtgctgcgc
     3181 gcgcagcggc ttccggaaag ctgggtgatc gcgctggtgg atcgcaaccg gcgcttcatc
     3241 gcccgcctcc cgccgatggc gccggggcga gcggtgtcgg agagcttccg cgccgcgatc
     3301 gagcgct
//
LOCUS       DATBSZ010000221        21514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_927000, whole genome shotgun sequence.
ACCESSION   DATBSZ010000221 DATBSZ010000000
VERSION     DATBSZ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21514
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927000"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VIG70_13790"
     CDS             complement(<1..816)
                     /locus_tag="VIG70_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8251673.1"
                     /translation="MTLDATHDPGLKSWVESANASGCDFPIQNLPFGIFKRKGARESP
                     RGGVAIGDQILDLAALAIKTGPALNGIAALGRSSWKRLRGQLSRGLSTKKRDKRFAKY
                     LLPMKQAQLFLPVDIGDYSDFFAGIHHAANMGKMLRPDNPLLPNYRWVPIGYHGRGSS
                     IVVSGTQVVRPHGQTKPPDAAAPFFGPSKRLDYEAELGFVIGPGNRLGRSIPVKDALN
                     HVFGVVLLNDWSARDIQAWEYQPLGPFLAKSFATTISPWIVTLEALEPYRCPAF"
     gene            complement(813..2099)
                     /gene="hmgA"
                     /locus_tag="VIG70_13795"
     CDS             complement(813..2099)
                     /gene="hmgA"
                     /locus_tag="VIG70_13795"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEY8251674.1"
                     /translation="MRAQRISYQSGFGNTFSTEAVKGALPIGRNSPQRAPLGLYAEVL
                     SGTAFTAPRAENQSTWLYKLRPSAVHGPYRRLEDALLRSGPFDEVETPPNRLRWDPLP
                     LPSRPTDFLQGLITIAGSGEPAAQAGVAVHVYRANRTMKDRCFWNADGELMFVPQQGS
                     IRLFTELGLLEAEPGEIAVVPRGMKFRVDVKGPARGYLCENYGAPFRLPELGPIGSQG
                     LAQARDFQAPTAAFEERGKCQVVAKFMGALWAADFAHSPLDVVAWHGDYVPYKYDLSR
                     FMAIGTISFDHADPSLFTVLTSPSGQAGVANCDFVIFPPRWQVAENTFRPPWFHRNVM
                     SELMGLVRGVYDAKAEGFLPGGVSIHNCMSAHGPDVTTYAKASEMELKPQKIEDTLAF
                     MWESRYVFRPTQFALGAPQLQKDYDRIWEGFRKNRR"
     gene            complement(2132..3139)
                     /locus_tag="VIG70_13800"
     CDS             complement(2132..3139)
                     /locus_tag="VIG70_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEY8251675.1"
                     /translation="MKVLVTGAAGFIGSTLAHALLRRGATVVGLDNLSDYYDVRLKQA
                     RLDRLTTHDQFRFERLDIASRDRMGTLFSLNKFDAVVHLAAQAGVRYSLTNPYAYVDA
                     NIVGFLNILEGVRHHGAKHLIYASSSSVYGANAKLPFSEHDNVDHPFSLYAATKKSNE
                     LMAHSYAHLYGLHCTGLRFFTVYGPWGRPDMALFLFTKGILSGRPIPVFNQGNMVRDF
                     TYVDDIVEGLVRVIDHPAAPNPSWRAEAPDPATSRAPYRIYNIGNNQPVKLLRYIEVL
                     EQALGKKAVLDLQPMQKGDVPATMADVSALEKAVGYRPGTPVEVGIPRFVEWYRSYYK
                     V"
     gene            complement(3238..4542)
                     /locus_tag="VIG70_13805"
     CDS             complement(3238..4542)
                     /locus_tag="VIG70_13805"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY8251676.1"
                     /translation="MSNLAIGSLGVAVLLAVLFLRVPIGVSLALVGVCGYAAVDGWRK
                     ALAMMGSVPFELASAYSLSVVPLFILMGAVASRAGMSRELFDAANAIFSGFRGALTNA
                     TIGACAAFGAICGSSIATAATFSKVAIPEMRRHGYEDGLAAGCVAAAGTLGILIPPSV
                     ILAIYSLVAEQSLSTLFAAALLPGLLLAALYILVVVIIARLKPEWTPKVAGMPLAQRL
                     RAAKDTWKLALLFFLAVVGIYLGWFSPTEAAAIAAFAAVVIGFATRTLSLRGLYHALL
                     DSVFSTAMLFFIIVGAFIFSRFVVLTRLPNELAAWVQAAGIGPLAIVLAVIALYFVLG
                     TFLEEVSTILITVPVILPLMTSIGYDGIWFGIFVTVMATIGLISPPVGLTVFVIQAQN
                     PDIQASKIYRGTLPFLVADFVLVGLLIAFPALALWLPAALKV"
     gene            complement(4553..4951)
                     /locus_tag="VIG70_13810"
     CDS             complement(4553..4951)
                     /locus_tag="VIG70_13810"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY8251677.1"
                     /translation="MVLLTVADIALRTFFSTPIQGMLELIELGLACTIFLALPAVFLR
                     DEHLVVDVVDHFVRPGVVRLLDLLGALISLGVLAVMAWQMLPLARTMHEFGDVTSDLS
                     IAKIWYWLPVLAGVIASAAATVVFVIRWKR"
     gene            complement(5010..6050)
                     /locus_tag="VIG70_13815"
     CDS             complement(5010..6050)
                     /locus_tag="VIG70_13815"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8251678.1"
                     /translation="MRKVLAVVLAAATSAALSGAAQAQDKVEVKVAEFVGAQHFMTKW
                     LMQWGEKLEKASNGRLVFKHFPGAQMAPTPAHYDLARTGQADVSWFLHGGTPGRFPLT
                     EIAHLPYMVGSAEIGTKVLNDAELRAKYLDAEHKGVKVLLLLTHQPGNIHTTKKPIRT
                     ADDMKGLRIRFSSPTIKDFVAALGGTAVGVQPNEQLEQLQKGTLDGTFIDYGGAGIAF
                     KMGGTLKYSTEMYSYISSFGVAMNPEFYNKLPADLKKLVDESMKGVEKEVGEGWDALD
                     TIGKKLIMDAGAQAIQLSKAEDARFRKVGSQVTEAKLKELEGKGLPARAVHKMMTELS
                     ARHAKTSKNFWN"
     gene            complement(6047..6895)
                     /locus_tag="VIG70_13820"
     CDS             complement(6047..6895)
                     /locus_tag="VIG70_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8251679.1"
                     /translation="MIPHETGRARSGELSIHFRRLGKKGQTPILIVHGLQYFSWDWLE
                     VAHALGAQREVLCMDMRGFGDSDWSAQKDYSVPAMAQDIVAVLDHVAWKHAILFGHSM
                     GGRSTTCAAAKNPARVEALALIDYTPENAPAGTRRTTEVVGNTPDSFPGLEAARQYLG
                     KKKDPERYRHYLKEENGRFSVKRDPHFRDQFKRALAGGERPKLGVDMWQLIGEVRCPI
                     LSVRGARSDMYAAETVTKMKAANPRLEVAEVDAGHDVAGEQPKGLLEAVEKWVRPIMA
                     GRRDFQ"
     gene            complement(6892..7662)
                     /gene="phhA"
                     /locus_tag="VIG70_13825"
     CDS             complement(6892..7662)
                     /gene="phhA"
                     /locus_tag="VIG70_13825"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEY8251680.1"
                     /translation="MKSDLRGDYSSAAPDFAVEQAWAKYTPEEHALYRRLFERQSRLV
                     PRYACPEWIEAIAGLDAAHEIPRFDEISKNLRSRTGWEIVAVPGLIPDEAFFTHLANR
                     RFPVTVWLRRPEEFDYIVEPDVFHDFFGHVPLLFDRTYADHLHEYGKGGLKAMRLDAV
                     KMLARLYWYTIEFGLMKTPAGVRAYGAGLLSSGGELAYCVDDPKPRRLPFDLERIMRT
                     EYIIDRYQETYFVIDSFEHLMRETAPDFTPIYARIRRS"
     gene            complement(7659..8744)
                     /gene="hppD"
                     /locus_tag="VIG70_13830"
     CDS             complement(7659..8744)
                     /gene="hppD"
                     /locus_tag="VIG70_13830"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY8251681.1"
                     /translation="MSSNPSNPIQTDGFEFVEYAAPDPAALGALFERMGFQAVARHRR
                     KNVLLYKQGDVNFIVNAEPRSFAQNFARRHGPSVCAIAFRVRDAASAYDKLIERGAWG
                     VENRPGPGELSIPAIKGIGDSLVYLVDRYPENDYGNTIYDVDFRPLPGFEPFWRGEAP
                     APRGAGLAYVDHLTHNVHRGRMQEWAEFYERLFNFREIRYFDIEGKLTGLKSKAMTSP
                     CGKIRIPINESADERSQIQEYLDQYHGEGIQHIALHTADIHKTVDSLKVEFLDTPDTY
                     YELLDKRVPGHGEPVDELKKRRILLDGAPGGGLLLQIFTKTMVGPIFFEIIQRKGDEG
                     FGEGNFKALFESMELDQVRRGVLNPAA"
     gene            complement(8759..9955)
                     /locus_tag="VIG70_13835"
     CDS             complement(8759..9955)
                     /locus_tag="VIG70_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY8251682.1"
                     /translation="MDIRGFRLTEVPEDFFADPYPYYAALRERAPLHELDHGSYFVSR
                     YDDVVAVYRHAHASSDKQVEFKPKFGDSPLYEHHTTSLVFNDPPLHARVRGLIMGALN
                     QRAIARMEAPVTQLVDRLLDRMAGECDFIDEFAARIPVEVIGNLLDVPRDERGPLRAW
                     SIAILSGLEPRLTPEMLERGNRAVLEFKDYLARLVEARRHSPGDFESDVLTRLIQGER
                     DGARLSALELYHNCIFLLNAGHETTTNLIGNGAWLLLSHPEQLQQLRSDPALLPLAIE
                     EMLRYEGPIQLNNRRLTGPLALAGTTLPEGSLLTLGIGAANRDARQFPDAERFDVARK
                     PNRHVAFGHGDHACAGMNVARLEARIAFSRLLRRFSKMEPCGVPERDRRIRFRGFRKL
                     PLRLGV"
     gene            10009..10542
                     /locus_tag="VIG70_13840"
     CDS             10009..10542
                     /locus_tag="VIG70_13840"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY8251683.1"
                     /translation="MQRVPLADFEPQLKKRLEELWGTPPNLYKALANHPQLVAAWTEF
                     SKTLRHDTRTPRALRELVILRGAQLQRSEYEWAQHLPMARKAGVSEEQIAGLASWRTS
                     PRFDARERAALALSEAVTQGQVSDAVHAEAKRHFDDHDYVELALVAAFYAMVGRMLDA
                     MGVQLEPDVRDHSPRLP"
     gene            10569..11237
                     /locus_tag="VIG70_13845"
     CDS             10569..11237
                     /locus_tag="VIG70_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEY8251684.1"
                     /translation="MAAFVTVLLCSNFIGAAKQATVNLPLLGAVTFSAGVLFFPISYI
                     FGDILTEVYGYGRDRRVVWAGFGALVFASVMAWVVISLPPAQSDFMKTYQANLEGVFG
                     NSWRIAAGSMIAFWCGSFSNSFVLAKMKLRTQGRWLWTRTIGSTVVGEAVDTSLFYVI
                     AFYGIWATNDLIKIAIAQYVLKTSWEIVMTPVTYRVVGFLKRAEQEDYYDRGTNFSPF
                     TLKT"
     gene            11304..11975
                     /locus_tag="VIG70_13850"
     CDS             11304..11975
                     /locus_tag="VIG70_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251685.1"
                     /translation="MENRTRYPADFRLGELLTRAGLVPRARLDAALERQAKHNGKLGA
                     LLVELALIEEAELYAVLALQSGLHRIEAEDVILFLRARLGDILLGAAAITEHQLLRAL
                     LQQERTGEPLGEILVRQGAISVAVREGALGFQRTLSSPFRDRFRLGRMLLEAGIVDSV
                     TLEAAIGRQRGARVKLGDALLEMGAITQEVLETFLKRQRRLLAALAAGMALAADAHGL
                     PARAC"
     gene            complement(11977..15495)
                     /locus_tag="VIG70_13855"
     CDS             complement(11977..15495)
                     /locus_tag="VIG70_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY8251686.1"
                     /translation="MELAKASLEDKYTIESGRAFLTGTQALIRLMMLQRSRDLSAGLN
                     TAGFVSGYRGSPLGGLDQALWRAQKHLESHHIKFQPGVNEDLAATAVWGTQQVGMFPG
                     ANYDGVFSMWYGKGPGVDRCGDVFKHANAAGTSKHGGVLALAGDDHAAKSSTLPHQSE
                     HIFKACLIPVLNPSNVQDYLDLGLHGFAMSRYSGCWIAFKCVTDVVESGASAEVNLNR
                     VQTKIPQDFTLPQGGLNIRWPDGILEQEARILDYKVYAALAYCRANGLDRIVWDSPRA
                     RFGICTTGKSYGDVMEALSSLGLTREICEDVGIRVYKVAMSWPLEPQGARRFAEGLEE
                     ILVVEEKRQLIEYQIKEELYNWKEGVRAPRVVGKFDDSGEWSRSGGQPAGTWLLPAHY
                     EHSPAMVARAIAQRLEKLGLTSHLGSQFRERLAFLDFKEKVLSRPRVTAIRQPYFCSG
                     CPHNTSTRVPEGSRATAGIGCHFMAVWMDRKTSTFTHMGAEGAPWIGQAPFTKEKHIF
                     ANLGDGTYFHSGLLAIRAAVAAKVSMTYKILYNDAVAMTGGQHHDGPLDPAMISRQIA
                     AEGVKPIVVVTDEPEKYPRGTNWAPGVTVRHRDELDAVQKELREIQGVSALIYDQTCA
                     SEKRRRRKKNEFPDPAVRAVINEMVCEGCGDCSVKSNCLSVEPLETEFGRKRTINQSS
                     CNKDYSCLKGFCPSFVTVEGGRLRKGKSAVAKVSDDFAALPDPELPASKTPYGILVTG
                     IGGTGVITIGQIIGVAAHLEGKGVSVLDMSGLAQKYGAVMSHVQIAAHPDAMHATRID
                     TGGASLVLGCDLVVTASAEALARMGQQRTRAVVNATVTPTAEFVKNPNWQLPGTDLQR
                     DITESVSEAAFIPATEIGAGLMGDAIATNMFMLGYAYQKGWIPVSGASLERAIELNGV
                     AVEFNKKSFVWGRRAALDLEKVKRIAMPADVIPIDQHFSRTLDELIERRVKFLTDYQN
                     AAYAGRYQALVDRARAVEKEKVNSSKLTEAVARYYAKLLAYKDEYEVARLHVAGDFRK
                     KIEGMFEGDYRVVYHLAPPLFARKDPTSGEPRKMRFGPWMLGAFGVLHRMKFLRGTAL
                     DPFGYTEERRTERALIREYEDTVERLLARLTPQNHAAAVQIAAIPDEIRGYGHIKART
                     VGPARKKREELLARYEAGPSVERVAA"
     gene            complement(15501..16448)
                     /locus_tag="VIG70_13860"
     CDS             complement(15501..16448)
                     /locus_tag="VIG70_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8251687.1"
                     /translation="MADALQELTIFAKIVGTGSLSAAARDLGMSPALVSRRLASLESR
                     LGVRLVNRTTRSLHLTDEGAAYFETCTRLLAEIQEADAAVSAGRAEPRGVLRVALPAS
                     FGNQHVAPLVPKFAERYPDVQLSLSLSDRAVNVVEEGFDLAIRIADLADSSLAARKLA
                     PNRRVVCASPGYLARHGAPRTPEDLAKHNCLATDFAMNWDYRGPDGKPGSVRVAGRYA
                     CDNWEVLREWALAGLGVALKSTWDVRRHLEDGSLLTLLPGYTFATDVAIYAVYPHRRH
                     LPAKTRAFVEFLAESFGPEPYWDRPRGAPKPRIKARAAV"
     gene            complement(16764..17288)
                     /locus_tag="VIG70_13865"
     CDS             complement(16764..17288)
                     /locus_tag="VIG70_13865"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8251688.1"
                     /translation="MERLKLNLGCGDKILPGYVNVDVAPTRAGQKPDVLCDLHRLEPF
                     EDKSADEVLAVHVVEHFWRWEVVDVLREWARVLKPRAAMILECPNLQSACEQFLRDPD
                     AFSGPGQEGQRTMWVFYGDPGWRDPLMVHRWGYTPRSLAKVMAQAGLVDIRQEPAQFK
                     LREPRDMRLVGVKA"
     gene            17531..18370
                     /locus_tag="VIG70_13870"
     CDS             17531..18370
                     /locus_tag="VIG70_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8251689.1"
                     /translation="MNYLKHFGLREQPFGITPDTSYFFACRSIQEALNTLCIAVSNGE
                     GFIKITGEVGTGKTLLCRKFLSTLGPGWVSAYIPNPNLEPRVVLLALAEELGLPGESI
                     GAHRLDKQLTQRLLDIARQGKRVVLCMDETQAMPLATLESVRLLTNLETEKRKLMQVV
                     LFGQPELDRKLASASVRQLRQRITFQYTLKALTRHEVMDYVAHRLTVAGYSGAALFSD
                     AALNAIHRASRGVPRLVNILAHKSLLLTYGEGARRADRRQVRAAVEDTPSARVPWWRW
                     KAA"
     gene            18367..19389
                     /locus_tag="VIG70_13875"
     CDS             18367..19389
                     /locus_tag="VIG70_13875"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8251690.1"
                     /translation="MSVINKMLQDLDRRQALGSAAETNVVRVPTTKSGGHEWFWRILI
                     VLLTAALAWMGWVAVQLMPRTPVVTELAFQAAAEARSRAAAKQAPTPAPATPAAAQTA
                     PVEQPKPASDSLRLAQQLETPVQERATEPSKPTEVQQPRVPAPQAATAPAKGTVADAH
                     FRSAALLLNRKRVSEAEDELVAALRADAAHVAARQAYVALLLEQQRVGVAHRVLHQGL
                     AINPEQPAFALEMARLYVGQREYDAALEVLDRAGPANATADFQAMRATVLQRLGRYAD
                     AVEAYQNALRGGTQPATTWVGLGISLEALGRRREAAQAYRRALTAGPIAAETRNYAET
                     RARALE"
     gene            complement(19396..20088)
                     /locus_tag="VIG70_13880"
     CDS             complement(19396..20088)
                     /locus_tag="VIG70_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8251691.1"
                     /translation="MRIAILEDDPDQLALLRRWISDDGHDVHAYLSGREAMKQAGRES
                     FDLFMLDWQVPDVSGAEVLMWLRANVSKSVPVLFVTVRDSEQDIVFALEHGADDYMVK
                     PVRRQELLARVHALLRRAYPSEEKKQLSFPPFEIDIQRGEVRKDGSRIDLTPKEFELT
                     VTLFRNIGRLMSRGHLQETVWGRTGDLATRTVDTHVSQVRKKLDLRPESGYRVVPIYN
                     YGYRLEKLSPAP"
     gene            20129..20932
                     /locus_tag="VIG70_13885"
     CDS             20129..20932
                     /locus_tag="VIG70_13885"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEY8251692.1"
                     /translation="MRVALLGGGTIARLVLEHRPPGIEIVALAGRTAASRGERLAREF
                     GVPYVIGRAALLAERPAAVVEAASHDAVREYLVPLLDAGVSVVVLSAGALANERLRTQ
                     AERAAAASGALFYVPSGGIGGLDALKTACLAGVDEVSIQVAKPPAAWKGIAYVESLGV
                     DLDRLQGATILFEGPAREGVPHFPQNVNIAAVLSLAGVGMDRTRLKVVADPGLSLNTH
                     TIRVSGASGRMTVILENVPAPENPKTSWLACYSALAALRALESKVRYGT"
     gene            20992..21078
                     /locus_tag="VIG70_13890"
     tRNA            20992..21078
                     /locus_tag="VIG70_13890"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21026..21028,aa:Tyr,seq:gta)
     gene            21164..21237
                     /locus_tag="VIG70_13895"
     tRNA            21164..21237
                     /locus_tag="VIG70_13895"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21196..21198,aa:Gly,seq:tcc)
     gene            21265..21339
                     /locus_tag="VIG70_13900"
     tRNA            21265..21339
                     /locus_tag="VIG70_13900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21297..21299,aa:Thr,seq:ggt)
     gene            21374..>21514
                     /locus_tag="VIG70_13905"
     CDS             21374..>21514
                     /locus_tag="VIG70_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8251693.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAMTHILAKKYGGEA
                     KNY"
BASE COUNT         3600 a         6950 c         7258 g         3706 t
ORIGIN      
        1 aaacgccggg cagcggtagg gctcgagcgc ctccagcgta acgatccacg gcgagatcgt
       61 ggtggcgaag ctcttggcga ggaatggccc gagcggctgg tattcccagg cctggatgtc
      121 gcgcgccgac cagtcgttga ggagcaccac gccgaagaca tggttcaacg catcctttac
      181 cggaatgctt cgtccgagcc gattgcccgg gccgatgacg aagccgagct cggcttcgta
      241 gtcaagtctc ttgctgggac caaagaaagg cgcggccgca tcgggcggct tcgtctggcc
      301 gtgcggccgc acgacctggg tgcccgacac cacgatcgac gagccgcggc cgtggtagcc
      361 gatcggcacc cacctgtaat tggggagcag cgggttgtcc ggccgcagca tcttgcccat
      421 gttggcagcg tgatggatgc cggcgaagaa gtcgctgtaa tcaccgatgt cgacgggcag
      481 gaagagttgt gcctgcttca tcggcagcag gtatttcgcg aaccgcttgt cgcgtttctt
      541 ggtcgacaat cctcgagaaa gctgcccgcg caggcgcttc cagctactgc ggcccagggc
      601 cgcgatcccg ttcagcgccg gcccggtctt gatggcgagc gccgccagat cgaggatctg
      661 gtcgccgatg gccacgccgc cgcgcggcga ttcgcgcgcc cctttgcgct tgaagatgcc
      721 gaacgggagg ttctggatcg ggaagtcgca gcccgacgcg ttcgccgact cgacccagct
      781 tttcagcccg ggatcatgcg tggcatcgag ggtcaccggc ggttcttcct gaatccttcc
      841 cagatcctgt cgtagtcctt ctgtagttgc ggcgcgccga gcgcgaactg ggttggccgg
      901 aacacgtagc gcgactccca catgaaggcg agcgtgtcct cgatcttctg cggcttcaat
      961 tccatttcac ttgccttggc gtatgtggtg acatccggcc catgtgccga catgcagttg
     1021 tggatcgaca ccccgccggg aaggaagccc tcggccttgg cgtcgtacac gccgcgcacc
     1081 aggcccatga gctcgctcat cacgttgcgg tggaaccacg gcggccggaa ggtattctcg
     1141 gcgacctgcc agcgcggcgg gaagatgacg aagtcacagt tcgccacgcc cgcctgtccc
     1201 gacggcgagg tgagcacggt aaagagcgac gggtcggcgt ggtcgaagct gatggtgccg
     1261 atcgccatga agcgcgagag gtcgtacttg tacggcacgt agtcgccgtg ccaggcgacg
     1321 acgtcgagcg gcgagtgcgc gaaatcggcc gcccagagcg cgcccatgaa cttggcgacg
     1381 acctggcact tgccccgctc ctcgaacgct gccgtcggcg cctggaagtc gcgcgcctgc
     1441 gcgaggccct gagagccgat cggaccgagc tcgggcaagc gaaaaggcgc accgtagttt
     1501 tcgcacaggt aaccgcgagc cgggcctttg acatccaccc tgaacttcat gcctcggggc
     1561 acgaccgcga tttctcccgg ttctgcctcg agcagcccaa gctcggtgaa caatcggatc
     1621 gacccctgct gcggtacgaa catcagctcg ccgtccgcgt tccagaagca gcgatctttc
     1681 atcgtgcggt tggcgcggta gacgtgcacc gccaccccgg cctgcgcggc gggctcgccc
     1741 gagcctgcaa tggtgatcaa cccctgaaga aaatctgtgg gccgcgacgg cagcgggaga
     1801 gggtcccagc gcagccggtt gggcggcgtc tcgacctcgt cgaacggtcc ggagcgcagc
     1861 agggcgtcct cgaggcgccg gtaagggccg tgcaccgccg aaggccgcag cttgtagagc
     1921 caggtgctct ggttttcggc gcgcggcgcg gtgaacgcag tgcccgatag cacctcggcg
     1981 tacaggccaa gcggcgcgcg ctgcggcgag ttgcgcccga tcggcagggc gcccttcacc
     2041 gcctcggtcg aaaaggtgtt accgaagccg ctctggtaac tgatccgctg ggcgcgcatc
     2101 gccggccgcg aaaattcccg ggggccgatc ctcagacttt gtagtagcta cggtaccact
     2161 cgacgaacct ggggatcccg acttccaccg gtgtgccggg tcgatagccc acggcctttt
     2221 ccagcgccga gacgtctgcc atggtcgcgg gtacgtcccc cttctgcatg ggctggagat
     2281 cgagcacggc cttcttaccc agtgcttgct cgaggacctc gatgtagcgc agcagcttca
     2341 cgggctgatt attgcctatg ttgtagatgc ggtagggtgc ccggctcgtt gcggggtccg
     2401 gagcctcggc ccgccacgac ggattcggcg cagccggatg atcgatgacg cgcaccaggc
     2461 cttcgacgat gtcatccaca tacgtgaagt cccgaaccat gtttccctgg ttgaacacgg
     2521 gaattgggcg gcccgaaagg attcccttgg tgaagagaaa gagcgccatg tccggccttc
     2581 cccatggccc atacacggta aaaaaccgta gcccggtgca atgcaagccg tagagatgcg
     2641 cgtagctatg cgccatcaat tcgttcgatt tcttcgttgc ggcgtacagc gaaaacggat
     2701 ggtcgacgtt atcgtgctcg gagaacggca gcttggcgtt ggcaccgtac accgagctcg
     2761 aagaagcgta gatcaggtgc tttgcgccat ggtggcgcac accctcgagt atgttgagga
     2821 acccaacgat attcgcgtcc acgtaggcgt aagggttggt gagcgaatag cgcacgccgg
     2881 cttgcgcagc caagtgcacg acggcgtcga acttgttgag cgagaacagg gtacccatcc
     2941 tgtcgcgcga tgcgatatcc aggcgttcga agcgaaactg gtcatgggtc gtcaagcggt
     3001 ccaggcgcgc ctgcttgagg cgtacgtcgt agtagtcgct gagattgtcc agaccgacga
     3061 cggtcgctcc gcgccgcagc aacgcatgcg ctagcgttga accgatgaat ccggcagcgc
     3121 cggtaacaag caccttcacc gaaaacgtcc ctagttgatc ccttggtttg ctcttttagc
     3181 gaagccacga ctctcggggc agctcggcca tcccagaggt ccggaatctt acctttctta
     3241 gactttcaga gccgccggaa gccagagggc gagcgcgggg aaggcgatga gtagcccgac
     3301 cagcacgaag tcggcgacca ggaagggcag ggtgccgcgg tagatcttgg acgcctggat
     3361 gtcggggttc tgcgcctgga tgacgaacac ggtgaggccg accggcgggc tgatgaggcc
     3421 gatggtggcc atcacggtca cgaatatccc gaaccagatg ccgtcgtagc cgatgctcgt
     3481 catgagcgga aggatcaccg ggaccgtgat caggatcgtg ctcacttcct cgaggaaggt
     3541 gccgagcacg aagtagagcg cgatcaccgc gagcacgatc gcgagcgggc cgatgccggc
     3601 ggcctgcacc caggcggcga gctcgttcgg cagccgcgtg aggacgacga agcgcgaaaa
     3661 gatgaaggcg ccgacgatga tgaagaagag catcgcggtc gagaacacgc tgtcgagaag
     3721 ggcgtgatag agcccgcgca gcgagagcgt gcgcgtcgcg aagccgatga cgaccgcggc
     3781 aaaggcggcg atcgccgccg cctcggtggg cgagaaccag ccgaggtaga tgccgaccac
     3841 cgcgaggaag aagaggagcg cgagcttcca ggtgtctttt gcggcgcgca ggcgctgcgc
     3901 gaggggcatg cccgcgactt tgggtgtcca ctcgggcttc aagcgggcga tgatgacgac
     3961 gaccaggatg tagagggctg ccagaagcag cccgggaagg agcgcggcgg cgaagagcgt
     4021 ggagagcgac tgctcggcga cgagcgagta gatcgcgagg atcaccgagg gcgggatcag
     4081 gatgccgagc gtgcccgccg cggcgacgca gcccgcggcg aggccgtcct cgtagccgtg
     4141 gcggcgcatc tccgggatcg ccaccttgga gaaggtcgcc gcggtggcga tcgacgagcc
     4201 gcagatcgcg ccgaacgcgg cgcaggcgcc gatggtggcg ttggtgagcg cgccgcgaaa
     4261 tccggagaag atggcgttgg ccgcgtcgaa cagctcgcgc gacatgccgg cgcgcgaggc
     4321 gactgcaccc atcaggatga agagcggcac gaccgatagc gagtacgcgc tcgccagctc
     4381 gaacggcacc gagcccatca tcgcgagcgc cttgcgccag ccgtcgaccg ccgcgtagcc
     4441 gcacacgccg acgagcgcga gagacacgcc gatcggcacc cgaaggaaca gcacggcgag
     4501 cagcaccgcc acgccgaggg agccgatggc gagattgctc atccgaattt gctcatcgct
     4561 tccagcgaat gacgaacacc acggtcgcag ccgcggaggc gatgaccccg gcgagcaccg
     4621 ggagccagta ccagatcttc gcgatcgaca ggtcggaggt gacgtcgccg aactcgtgca
     4681 tcgtccgcgc gagcggcagc atctgccagg ccatgacggc gagcacgccg agcgagatca
     4741 gcgcgccgag caagtccagc agccgcacta cgcccgggcg aacgaagtgg tcgaccacgt
     4801 cgacgaccag gtgctcgtcg cgcaagaaca ccgcgggcag ggcgagaaag atggtgcagg
     4861 cgaggccgag ctcgatcagc tcgagcatgc cctggatcgg ggtgctgaag aacgtgcgca
     4921 gcgcaatgtc cgccacggtg agcagcacca tggccgcgag aaaaaccgcc gcgagcgtcc
     4981 cgaagaccgt cgcggcgagc ttcagacttc tagttccaga agttcttcga ggtcttggcg
     5041 tgccgggcgg agagctcggt catcatcttg tgcaccgcgc gcgccggcag gcccttgcct
     5101 tcgagctcct tcagcttcgc ctcggtgacc tgcgagccga ccttgcggaa gcgcgcgtcc
     5161 tcggccttgg agagctggat cgcctgggcg ccggcgtcca tgatcagctt cttgccgatg
     5221 gtgtcgagtg cgtcccagcc ttcgccgact tccttctcca cgcctttcat cgactcgtcg
     5281 accagcttct tcaggtcggc gggaagcttg ttgtagaact ccgggttcat cgccacgccg
     5341 aacgacgaga tataggagta catctcggtc gagtacttga gcgttccgcc catcttgaac
     5401 gcgatgccgg cgccgccata gtcgatgaag gtgccgtcga gtgtgccctt ctgcagctgc
     5461 tcgagctgct cgttcggctg caccccgacc gcggtgccgc cgagcgcagc gacgaagtcc
     5521 ttgatggtcg gcgaggaaaa ccggatgcgc agccccttca tgtcgtcggc ggtgcggatc
     5581 ggcttcttgg tggtgtgaat gttgcccggc tggtgcgtga gcagcagcag caccttcacg
     5641 cccttgtgct cggcgtccag atatttggcg cgcagctcgg cgtcgttcag caccttggtg
     5701 ccgatctccg cgctgccgac catgtagggc aggtgggcaa tctcggtgag cgggaagcgg
     5761 cccggcgtgc cgccgtgcag gaaccacgac acgtcggcct gtccggtgcg cgcgagatcg
     5821 tagtgcgccg gtgtcggcgc catctgcgcg ccggggaaat gcttgaagac gagccggccg
     5881 ttggacgcct tctcgagctt ctcgccccat tgcatcagcc acttcgtcat gaagtgctgc
     5941 gcgccgacga attcggccac cttgacttcg accttgtcct gcgcctgcgc ggccccgctc
     6001 agtgccgccg atgtcgccgc cgcaagtacg acggccagta cttttctcat tggaaatccc
     6061 tccgcccggc cattataggg cgaacccatt tctcgaccgc ttcaaggagc cccttcggct
     6121 gctcgccggc gacgtcgtgc ccggcatcga cttcggccac ctcgagccgc gggttcgcgg
     6181 ctttcatttt cgtgacggtc tcggcggcat acatgtccga gcgcgcgccg cgcaccgaca
     6241 ggatcgggca gcgcacctcg ccgatcagct gccacatgtc gaccccgagc ttgggccgct
     6301 cgccgccggc gagggctcgc ttgaactggt cgcggaagtg cggatcgcgc ttgacgctga
     6361 agcggccgtt ttcctccttc aggtagtggc gatagcgctc gggatccttc ttctttccga
     6421 ggtactgccg cgctgcttcc aggccgggaa acgaatcggg tgtattgccc acgacctcgg
     6481 tggtgcgccg ggtgcccgcg ggcgcattct cgggcgtgta gtcgatgagc gcgagcgcct
     6541 ccacgcgcgc cgggttcttc gccgcggcgc aagtggtgct gcgcccgccc atcgaatggc
     6601 cgaagaggat cgcgtgcttc caggcgacgt ggtccagcac ggctacgatg tcctgcgcca
     6661 tcgccggcac cgagtagtcc ttctgcgcgc tccagtccga gtcgccgaag ccgcgcatgt
     6721 ccatgcacag tacttcgcgc tgggcgccga gcgcgtgcgc cacttcgagc cagtcccacg
     6781 agaagtactg cagcccatga acgatcagga tcggggtctg gccctttttt ccaagccggc
     6841 ggaaatggat cgagagctcg cccgagcgtg cgcgcccggt ttcgtggggg atcatgagcg
     6901 ccggatgcgc gcgtagatcg gcgtgaagtc gggcgcggtc tcgcgcatca ggtgctcgaa
     6961 ggagtcgatc acgaagtaag tctcctggta gcggtcgatg atgtactcgg tgcgcatgat
     7021 gcgctcgagg tcgaacggca gccgccgcgg cttcggatcg tcgacacagt acgcgagctc
     7081 cccgcccgag gacagcagcc ccgccccgta cgcccgcacg ccggcagggg ttttcatgag
     7141 gccaaactct atcgtgtacc agtaaagccg ggcgagcatc ttcaccgcgt cgaggcgcat
     7201 cgccttcagg ccgcccttgc cgtactcgtg gaggtggtcg gcgtaggtgc gatcgaacag
     7261 cagcggcacg tgaccgaaga aatcgtggaa cacgtcgggc tcgacgatgt agtcgaactc
     7321 ctcgggccgg cgcagccaca ccgtgaccgg aaagcgtcgg ttggcgaggt gggtgaagaa
     7381 cgcttcgtcg ggaatgaggc ccgggacggc gacgatctcc cagccggtcc tgcttctcag
     7441 gtttttcgag atctcgtcaa accgggggat ctcatgcgcg gcgtcgagcc cggcaatcgc
     7501 ctcgatccat tccgggcacg cgtagcgcgg cacgagcctc gactgacgct cgaacaggcg
     7561 ccggtaaagc gcgtgctcct cgggggtgta cttcgcccac gcctgctcga ctgcgaaatc
     7621 aggggcggcg ctagagtagt cgccccttag atcggatttc atgccgcggg attgagaacc
     7681 ccgcggcgca cctggtcgag ctccatcgat tcgaacagcg ccttgaagtt gccctcgccg
     7741 aagccttcgt cgccctttcg ctggatgatc tcgaagaaga tcggccccac catcgtcttg
     7801 gtgaagatct gaaggagcaa tccgcccccg ggggcgccgt cgagcaggat ccggcgcttc
     7861 ttcagctcgt ccaccggctc gccgtggccg ggaacgcgct tgtcgagcag ctcgtagtac
     7921 gtgtccggcg tatcgaggaa ttcgactttc agggaatcga cggtcttgtg gatgtctgcg
     7981 gtgtgcagcg cgatgtgctg gatgccttcg ccgtggtact ggtcgaggta ctcctggatc
     8041 tgcgagcgct cgtcggcgga ctcgttgatc gggatgcgga tctttccgca ggggctggtc
     8101 atcgccttcg acttgagccc ggtgagcttg ccctcgatgt cgaaatagcg gatctcgcgg
     8161 aagttgaaca ggcgctcgta gaattccgcc cattcctgca tgcgcccacg gtgcacgttg
     8221 tgcgtcaggt gatcgacata ggcgagcccc gcgccgcgcg gcgccggcgc ttcgccgcgc
     8281 cagaaaggct cgaagccggg cagcggcctg aagtcgacgt cgtagatcgt gttgccgtag
     8341 tcgttttcgg ggtagcggtc gacgaggtac acgagcgaat cgccgatgcc cttgatcgcc
     8401 gggatgctga gctcgcccgg cccggggcgg ttctccacgc cccacgcgcc gcgctcgatg
     8461 agcttgtcgt aggcgctcgc cgcgtcgcgc acgcgaaacg cgatcgcgca cacgctcggg
     8521 ccgtggcggc gcgcgaagtt ctgcgcgaac gagcgcggct ccgcgttgac gatgaagttg
     8581 acgtcgccct gcttgtagag cagcacgttc ttgcggcggt ggcgcgcgac cgcctggaag
     8641 cccatgcgct cgaagagcgc gccgagcgcc gccgggtcgg gggcggcgta ctcgacgaac
     8701 tcgaaaccgt cggtctgaat cgggttggaa gggttagacg acatggccat gatccatttt
     8761 agaccccgag gcggagcggg agtttgcgaa agccgcggaa acggatgcgc cggtcgcgct
     8821 cggggacccc gcacggttcc atcttggaga atctcctcaa caggcggctg aaggcgatgc
     8881 gcgcctccag gcgcgcgacg ttcatgccgg cgcaggcatg gtcgccgtgg ccgaaggcga
     8941 cgtggcggtt gggcttgcgc gctacgtcga agcgctcggc gtccggaaac tggcgcgcgt
     9001 cgcggttcgc cgcgccgatt cccagggtga ggagcgaacc ctcgggcaga gtcgtgcccg
     9061 ccagcgcgag cgggccggtg aggcggcggt tgttgagctg gatcgggccc tcgtagcgca
     9121 gcatttcctc gatcgcgagc ggcagcagcg cgggatcgct tctcagttgc tgcaattgct
     9181 ccgggtggga gagaagcagc caggcgccgt tgccgatcag gttggtggtg gtctcgtggc
     9241 cggcgttcag gagaaagatg cagttgtggt agagctcgag cgcgctcagc cgcgcgccgt
     9301 cgcgctcgcc ttggatgagc cgcgtcagca cgtcgctttc gaagtcgccg gggctgtggc
     9361 gccgcgcctc caccagccgg gcgagatagt ccttgaactc gagcaccgca cggttgccgc
     9421 gctcgagcat ctcgggggtc aggcgcggct cgagccccga caggatggcg atcgaccacg
     9481 cgcgcagcgg gccgcgctcg tcccgcggca cgtcgagcag gttgccgatc acctcgaccg
     9541 ggatgcgcgc ggcgaactcg tcgatgaagt cgcattcccc ggccatgcgg tcgagcagcc
     9601 ggtccacgag ctgcgtcacc ggggcttcca tgcgcgcgat ggcgcgctgg ttgagcgcgc
     9661 ccatgatcag gccgcggacg cgcgcatgca gcggcgggtc gttgaatacc aggctcgtag
     9721 tgtgatgctc gtaaagcggc gagtcgccga atttcggctt gaactcgacc tgcttgtccg
     9781 agctcgcgtg cgcgtggcga tagaccgcga ccacatcgtc gtagcgcgag acgaagtagc
     9841 tgccgtggtc cagctcgtgc agcggagcgc gctcgcgcag ggcggcatag tacggatacg
     9901 ggtcggcgaa gaaatcctcc ggcacttcgg tcagccggaa gccgcggatg tccaccaggc
     9961 tattatgcgc gccaagagaa ttggcctcac agccgttatc attcgcgcat gcagcgtgtg
    10021 ccgcttgcgg acttcgagcc gcagctcaag aaaaggctcg aggagctctg ggggacgccg
    10081 cccaacctct acaaggcgct agccaaccat ccgcagctcg tcgccgcatg gaccgagttc
    10141 tcgaagacgc tgcgccacga cacgcgcacc ccgcgcgcgc tgcgcgagct cgtaatcctg
    10201 cgcggcgcgc agcttcagcg ctcggagtac gagtgggcgc agcacctgcc gatggcgcgc
    10261 aaggcgggcg tttccgagga gcagatcgca ggcctcgctt cctggaggac ttccccgcgg
    10321 ttcgatgcgc gcgagagggc cgcgctggcg ctgagtgaag ccgtgaccca ggggcaggtc
    10381 agcgacgcgg tgcacgccga agcgaagcgt cacttcgacg accacgacta cgtcgagctg
    10441 gcgctcgtcg ctgcgttcta cgccatggtc gggcgcatgc tcgacgccat gggcgtgcag
    10501 ctcgagccgg acgtccgtga ccactctccg aggttgcctt gagaacgtat cgctactacg
    10561 acttcgtgat ggcggctttc gtcacagtgc tgctgtgctc gaacttcatc ggcgcggcga
    10621 agcaggcgac ggtgaacctt ccgttgctcg gcgccgtgac cttcagcgcc ggggtcctgt
    10681 tcttcccgat ctcctacatc ttcggcgaca tcctcaccga ggtgtacggc tacggccgcg
    10741 accgccgcgt cgtgtgggcc gggttcggcg cgctggtgtt cgcgtcggtc atggcctggg
    10801 tggtcatcag cctgccgccg gcgcagagcg atttcatgaa gacctaccag gccaacctcg
    10861 agggggtgtt cggcaacagc tggcgcatcg cggcggggtc gatgatcgcc ttctggtgcg
    10921 gcagcttctc gaattccttc gtgctggcga agatgaagct gcgcacccaa ggccggtggc
    10981 tctggacccg caccatcggc tcgacggtcg tcggcgaggc ggtcgacacc tcgcttttct
    11041 acgtgatcgc gttctacggc atctgggcga cgaacgacct catcaagatc gcgatcgcgc
    11101 agtacgtgct gaagacctcg tgggagatcg tcatgacccc ggtgacctac cgcgtggtcg
    11161 ggttcctgaa gcgcgccgag caggaggact actacgaccg cggcaccaac ttcagcccgt
    11221 tcaccctgaa gacataattt caccttttgc tgcggcacaa gttcctcgac cctcccgggc
    11281 ggcgggaacg atcatcgccg cgcttggaaa accgcacccg atatccggcc gacttccggc
    11341 tcggcgagct cctcactcgc gccggcctcg tgccgcgggc gcgcctcgac gccgccctcg
    11401 agcggcaagc gaagcacaac ggcaagctcg gcgcgctgct ggtcgagctc gcgctgatcg
    11461 aggaggccga gctctacgcg gtgctcgccc tgcagtcggg cctgcaccgg atcgaagccg
    11521 aagacgtgat cctcttcctg cgcgcccgcc tcggcgacat cctcctcggc gcggcggcga
    11581 ttaccgagca tcagctcctg cgcgcgctcc tgcagcagga gcgcaccggc gagcccctcg
    11641 gcgagattct cgtacgccag ggcgcgatca gcgtggccgt gcgcgaaggc gcgctcggct
    11701 tccagcgcac cctgtcgagc ccgttccgcg accgcttccg cctcggcagg atgctgctcg
    11761 aagcgggcat cgtcgactcg gtcacccttg aggccgcgat cgggcgccag cgcggcgcgc
    11821 gcgtcaagct cggcgacgcg ctgctcgaga tgggcgccat cacgcaggaa gtgctcgaga
    11881 cctttctcaa gcgccagcgc cggctgctcg cggcgctcgc tgctggcatg gcgctcgccg
    11941 ccgacgcgca cggcctgccc gcgcgcgcct gctagcctag gccgcgaccc gctccaccga
    12001 agggcctgct tcgtagcgcg cgagaagctc ttcgcgcttc ttgcgcgccg ggcccacggt
    12061 gcgcgccttg atgtggccat agccgcggat ctcgtccggg atggcggcga tctgcaccgc
    12121 agccgcatgg ttctgcggcg tcagcctggc gagcaggcgc tcgaccgtgt cttcgtactc
    12181 gcggatcagc gcgcgctcgg tgcgccgctc ctcggtgtag ccgaagggat ccagggccgt
    12241 tccccgcaga aatttcattc ggtgaagaac tccaaaggcc cccagcatcc acggcccgaa
    12301 acgcatcttg cgcggctcgc cgctcgtcgg gtccttgcgc gcgaaaagcg gcggcgcgag
    12361 gtggtacacg acgcgatagt cgccctcgaa catgccctcg atcttcttgc ggaagtcccc
    12421 cgcgacgtgc agccgcgcga cctcgtactc gtccttgtag gcgaggagct tcgcgtagta
    12481 gcgcgccacc gcttcggtca gcttcgagga attgaccttt tccttttcaa ccgcgcgggc
    12541 gcgatcgacc aatgcttgat aacggcccgc gtaggcggcg ttctggtagt cggtgaggaa
    12601 cttcactcgc cgctcgatca gctcgtccag ggtgcgcgaa aagtgctggt cgatcggaat
    12661 cacatcggct ggcatggcga tgcgcttcac cttctccagg tcgagcgcgg cgcgccgtcc
    12721 ccagacgaag ctcttcttgt tgaactccac cgcgaccccg ttcagctcga tcgcgcgctc
    12781 gagggacgcg cccgagaccg ggatccagcc cttctggtag gcgtagccga gcatgaacat
    12841 gttggtggca atggcgtcgc ccatcaggcc ggcgccgatc tcggtcgccg ggatgaaggc
    12901 cgcctcgctc accgattcgg tgatgtcgcg ctgcaggtcg gtgcccggca gctgccagtt
    12961 ggggtttttc acgaactcgg cggtcggcgt cacggtggcg ttgaccacgg cgcgcgtgcg
    13021 ctgttggccc atcctggcga gcgcttcggc gctcgcggtg accaccaggt cgcagccgag
    13081 cacgaggctc gcgccgccgg tgtcgatgcg cgtcgcgtgc atcgcgtcgg ggtgcgccgc
    13141 gatctgtacg tgcgacatca ccgcgccgta cttctgcgcg aggcccgaca tgtcgagcac
    13201 gctgacgcct ttgccctcga ggtgcgcggc gacgccgatg atctggccga tcgtgatgac
    13261 cccggttccg ccgatcccag tgacgaggat gccgtagggg gttttcgaag cgggcagctc
    13321 ggggtcggga agcgcagcga agtcatcact cactttcgcc accgccgatt tgcccttgcg
    13381 cagccggccg ccctcgacgg taacgaagct cgggcagaag cccttcaggc acgagtagtc
    13441 cttgttgcag gaggactggt tgatggtgcg ctttcggccg aactcggtct cgagcggctc
    13501 gacagagagg cagttggatt tcacgctgca atcgccgcag ccttcgcaca ccatctcgtt
    13561 gatcaccgcg cgcaccgcgg gatcggggaa ctcgttcttc ttgcgccggc ggcgcttctc
    13621 ggaggcgcag gtctggtcgt agatgagcgc ggacacgcct tggatttcac gtagttcttt
    13681 ttgaactgca tcaagctcgt cgcgatggcg cacggtgacc cccggcgccc agttggtgcc
    13741 gcgcgggtat ttctccggct cgtcggtgac caccacgatc ggcttgacgc cctcggcggc
    13801 gatctggcgc gagatcatcg ccgggtccag agggccgtcg tggtgctggc cgccggtcat
    13861 cgccaccgcg tcgttgtaga ggatcttgta ggtcatgctg accttggccg cgacggcggc
    13921 gcggatcgcg agcagccccg agtggaagta agtgccgtcg ccgaggttcg caaagatatg
    13981 cttttccttg gtgaacggcg cctggccgat ccatggcgcg ccctcggcgc ccatgtgggt
    14041 gaaggtcgag gtcttgcggt ccatccacac cgccatgaag tggcagccga tgccggcggt
    14101 ggcgcggctg ccctcgggca cgcgggtgga cgtgttgtgc gggcagcccg agcagaaata
    14161 gggttgcctt atcgccgtca ccctcgggcg cgacaggacc ttttccttga aatcgaggaa
    14221 ggcgagcctt tcccggaatt ggcttccaag gtgtgaagtc aaacccaact tctccagcct
    14281 ctgagcaatc gcacgcgcga ccatcgccgg cgagtgctcg tagtgcgcgg gcagcagcca
    14341 ggtgccggcg ggctgcccgc cgctgcggct ccactcgccg ctgtcgtcga acttgccgac
    14401 cacgcgcggc gcgcgcaccc cttccttcca gttgtagagc tcttccttga tctggtattc
    14461 gatcagctgg cgcttctcct cgactaccag gatctcttcc aggccctcgg cgaagcgccg
    14521 cgcgccttgc ggctcgagcg gccaggacat ggcgaccttg tagacgcgga ttccgacgtc
    14581 ttcacaaatt tctcttgtaa gacccaaaga cgaaagggcc tccatgacat ccccgtacga
    14641 cttgccggta gtgcagatgc caaagcgcgc gcgcggcgag tcccacacga tgcggtccag
    14701 gccgttggcg cggcagtagg cgagcgcggc gtagaccttg tagtcgagga tgcgcgcttc
    14761 ctgctcgagg atgccgtcgg gccagcggat gttcagcccg ccttggggaa gcgtgaagtc
    14821 ctgcgggatc ttggtctgaa ctcgattcaa attgacttcg gcggatgcgc cggactccac
    14881 gacatcggtg acgcatttga aggcgatcca gcagcccgag tagcggctca tcgcaaagcc
    14941 gtgcagcccc agatcgaggt agtcctgcac gttcgagggg ttgagcaccg ggatgaggca
    15001 ggctttgaag atgtgctccg actggtgcgg cagcgtcgag gacttcgcgg cgtggtcgtc
    15061 gccggcgagc gcgagcacgc cgccgtgctt cgaagtgccg gcggcgttgg cgtgcttgaa
    15121 gacgtcgccg cagcggtcga cgccggggcc cttgccgtac cacatcgaaa acacgccgtc
    15181 gtagttcgcg cccgggaaca tgccgacctg ctgcgtgccc cagaccgcgg tcgcggcgag
    15241 gtcctcgttc acgcccggct ggaatttgat gtggtggctc tcgaggtgct tctgcgcgcg
    15301 ccaaagcgcc tgatcgaggc caccgagcgg cgagccgcgg tagcccgaga cgaagccggc
    15361 ggtgttcagc cccgcgctca ggtcgcggct gcgctgcagc atcatcaggc ggatcagcgc
    15421 ctgcgtcccg gtcagaaagg cccggccgct ctcgatcgtg tacttgtctt cgagactggc
    15481 tttagcaagt tccatagcag ttagacagcg gctcgggctt tgattctcgg cttcggcgcc
    15541 ccacgcgggc ggtcccagta tggctcaggg ccgaacgact cggccaggaa ttcaacgaac
    15601 gcacgagtct tggccggcag gtgacgccgg tgaggataga ccgcgtaaat ggccacatct
    15661 gtcgcgaagg tatagcccgg aagaagcgtg agcagcgaac cgtcctcgag gtgacggcgc
    15721 acgtcccagg tggacttgag cgcgacgcca agcccggcaa gggcccactc gcgcagcact
    15781 tcccagttgt cgcacgcgta gcggcccgca accctcaccg agcccggctt cccgtcgggc
    15841 ccgcgatagt cccaattcat cgcgaagtct gtggcgaggc aattgtgctt cgccaggtcc
    15901 tcgggcgtgc gcggcgcgcc gtggcgcgcc aggtagccgg ggcttgcgca caccacgcgg
    15961 cggttcggcg cgagtttgcg ggcggcgagc gacgagtcgg ccaggtcggc gatacggatc
    16021 gcgaggtcga agccctcttc gaccacgttc accgcccggt cggacaggct aagggagagc
    16081 tgaacgtcgg gatagcgctc ggcgaacttc ggcaccagcg gcgcgacgtg ctggttgccg
    16141 aacgaggcgg gcagagcgac tcgcagcacc ccgcgcggct cggcgcgccc cgccgacacc
    16201 gccgcgtcgg cctcctggat ctctgcgagg agccgggtgc aggtctcgaa atacgcggca
    16261 ccttcgtcgg tgaggtgcag gctgcgggtc gttcggttga ccaggcgcac ccccagccgc
    16321 gattcgagcg aggcgaggcg ccggcttacg agggcaggcg acatgccgag gtcccgggcc
    16381 gcggcagaga ggctgccggt gccgacgatc ttggcgaaaa tcgtaagctc ttgaagggcg
    16441 tccgccatct ttgcagattc tgcaaaagtg ttttacagct gcagcgtatt ctaaaccaac
    16501 gagcaaagac ctaagattgt tgcgttgcag cagcccggtt ggttcctcct cctcctccaa
    16561 taccgtcggg ctgactcagg gggcaggccc aaagcctgcc ccctttattt tttcaggtag
    16621 tagccgcgag gggcgaaggt gcagccgaac ttgtcctccc gggcgggatc ggccgtcagc
    16681 aggcccggat tcaagccggt agctgagccg cttccatacc gcggtctcgt agtaccaccg
    16741 tagataggct tctccctccg cgttcatgct ttgaccccga ccagccgcat gtcgcgcggt
    16801 tcgcgcagct tgaactgcgc cggctcctgg cggatgtcga ctaggccggc ctgcgccatc
    16861 accttggcga ggctgcgcgg ggtgtagccc caacggtgca ccatgagcgg atcgcgccag
    16921 cccgggtcgc cgtagaacac ccacatcgtg cgctggccct cctggccggg cccggagaac
    16981 gcatccgggt cgcggaggaa ctgctcgcag gcgctctgca ggttcgggca ctcgagaatc
    17041 atcgccgcgc gcggcttgag tacgcgcgcc cactcgcgca gcacgtcgac cacctcccag
    17101 cgccagaagt gctcgaccac atggaccgcg aggacttcgt cggcgctctt gtcctcgaac
    17161 ggctcgaggc ggtgcaggtc gcagaggaca tccggcttct ggccggcgcg ggtcggtgcc
    17221 acgtcgacgt tgacgtagcc gggcaggatc ttgtcgccgc agccgaggtt gagcttcaag
    17281 cgctccaaga ctagttcctc ggggcgaatt gcgctggtcg acgaatgttg ctgcggtcga
    17341 cctcccggca gccacggcgt gccgatggaa aagacgattg actacaatct gttattgcta
    17401 taacttcaag tgattgcttg gtcggcgggg gtaggcttac gcgggtaatg aggaaaaggc
    17461 cacgtgcgcc aacttgttgc gttctctcgg acctcggcgc taccggcggc cgcaaggaga
    17521 ggctgaagta gtgaactacc tgaagcactt tggcctgcgc gagcagcctt tcggcattac
    17581 gcccgacacg agctacttct tcgcttgccg cagcatccag gaagcactga acacgctctg
    17641 cattgctgtt tcgaacggcg agggcttcat caagatcacc ggcgaagtcg gcaccggcaa
    17701 gacgcttctg tgccgcaagt tcctgtccac gctaggcccc ggctgggtga gcgcctacat
    17761 accgaatccg aatctcgagc cgcgcgttgt cctgctcgcg ctcgccgagg agctcggcct
    17821 gccgggtgag tcgattgggg cgcaccggct ggacaagcag ctgacgcagc gcctgctcga
    17881 catcgcccgc cagggcaagc gcgtcgtact gtgcatggac gagacgcagg caatgccgct
    17941 cgctacgctg gaatcggtgc ggctcctaac caacctcgag accgagaagc gcaagctcat
    18001 gcaggtggtg ttgttcggcc agcccgagct cgatcgcaag ctcgcctcgg cgtcggtgcg
    18061 gcagctgcgc cagcgcatta ccttccagta cacgctcaag gcgctcaccc ggcacgaggt
    18121 gatggactat gtcgcgcacc gcctcacggt cgccgggtat tccggcgccg cgctgttcag
    18181 cgacgctgcg ttgaacgcga tccatcgcgc cagccgcggc gtgccgcgcc tggtgaacat
    18241 cctcgcccac aaatcgctgt tgctgaccta cggcgaaggc gctcgccgcg ccgaccgccg
    18301 gcaagtgcgc gccgcggtcg aggacacccc ttcggctcgc gtgccgtggt ggcggtggaa
    18361 ggccgcatga gcgtcatcaa caagatgctg caggatctcg accggcggca ggcgcttggg
    18421 tccgccgctg aaacgaacgt ggtgcgggtg ccgaccacca agtccggcgg ccacgaatgg
    18481 ttttggcgga ttctgatcgt gctgctcacc gcagcgctcg cgtggatggg ctgggtggcg
    18541 gtccagctga tgccgcgcac gccggtcgtc accgagctcg ctttccaggc cgctgccgag
    18601 gcgcggagcc gggccgcagc gaagcaggcg ccgacaccgg cgcccgcgac tcctgctgcg
    18661 gcacaaaccg caccggtgga acagccgaag cccgccagcg attcgctgcg gcttgcgcag
    18721 cagctcgaaa cgccggtgca ggaaagggcg acggagccgt ccaagccgac tgaggtgcaa
    18781 caaccccgag tcccggcacc ccaggcggca acagcgccgg caaaggggac cgtcgccgac
    18841 gcgcatttcc ggagtgccgc cctgctcctc aatcgcaagc gggtgagcga ggctgaggat
    18901 gagctcgtcg ctgcattgcg cgccgacgcc gcgcacgtgg cggcccggca ggcgtatgtc
    18961 gcgctgctgc tcgagcagca gcgtgtcggc gtcgcgcacc gggttctgca tcaagggttg
    19021 gcgatcaatc ccgagcaacc cgcgtttgcc ctcgagatgg cgcgccttta cgtgggccag
    19081 cgcgagtacg acgcagcttt ggaggtcctg gatcgagcgg gacccgccaa cgccaccgcc
    19141 gatttccagg cgatgcgcgc taccgtgctg cagcggctcg gccgctatgc ggacgccgtc
    19201 gaggcctatc agaacgcgtt gcgcggcggt acgcagccgg cgacgacctg ggtcggcctt
    19261 ggcatttccc tggaagcgtt gggccgccga agagaggcgg cgcaggccta tcggcgcgcg
    19321 cttaccgcag gtccgatcgc ggcggaaacc cggaactacg cggaaactcg agcgcgggcg
    19381 ctggagtagg cgaagctagg gggccgggga aagcttctcg aggcggtagc cgtagttgta
    19441 gatcggcacc acccggtagc ccgattccgg gcgcagatcg agcttcttgc gaacctggct
    19501 cacgtgggtg tcgaccgtgc gcgtggccaa gtcgccggtg cggccccaca cggtttcctg
    19561 caggtggccg cgcgacatca gccgcccgat attgcggaac agcgtcaccg tgagctcgaa
    19621 ttccttcggg gtgaggtcga tgcggctgcc gtccttgcgc acctcgccgc gctggatgtc
    19681 gatctcgaaa ggcgggaacg agagctgctt tttctcctcg ctcggatagg cgcggcgcag
    19741 cagcgcgtgc acgcgggcga gcagctcctg gcggcgcacc ggcttcacca tgtagtcgtc
    19801 ggcgccgtgc tccagggcga acacgatgtc ctgctcggaa tcgcgtaccg tgacgaacag
    19861 cactggcacg ctcttcgaga cgttggcgcg cagccacatc aggacctcgg caccggacac
    19921 gtcgggtacc tgccagtcca gcatgaagag atcgaagctc tcgcgcccgg cctgcttcat
    19981 cgcctcgcgc ccgctgaggt acgcgtgcac gtcgtggccg tcgtcggaga tccagcgccg
    20041 cagcagcgcc agctggtcgg gatcgtcttc tagaatcgcg atacgcacgc aacccccttg
    20101 tttgtggcca atgatactgg agagaaccat gcgcgtcgcg ttgctgggtg gcggcaccat
    20161 cgcgcggctg gtcctggagc accgcccgcc cggaatcgag atcgtcgcgc tggccgggcg
    20221 cacggcggcc tcgcgcggcg agcgtctggc gcgcgagttc ggcgtgccct acgtgatcgg
    20281 gcgcgcggcc ctgcttgccg agcggccggc ggcggtggtc gaggcggcat cccacgatgc
    20341 ggtgcgcgag tatctcgtgc cgctgctaga cgcgggcgtg agcgtggtcg tcctttcggc
    20401 gggcgcgctc gcgaacgagc gcctgcgaac gcaggccgag cgcgccgcgg ccgccagtgg
    20461 cgccctgttc tacgtgccct cgggcggcat cggagggctc gacgcgctga agaccgcctg
    20521 cctggcgggc gtggacgagg tctccatcca agtggcgaag cccccggcgg cctggaaggg
    20581 aatcgcctat gtggagagcc tcggcgtcga cctcgaccgg ctgcaagggg cgacaatcct
    20641 gttcgaggga ccggcgcgcg agggcgtgcc gcactttccg cagaacgtca acatcgcggc
    20701 cgtgctgtcg ctcgccgggg tcggcatgga ccgcacccgc ctgaaggtcg tcgccgaccc
    20761 gggactcagc ctgaataccc acacgattcg tgtgtcgggg gcctcggggc gcatgacggt
    20821 gatcctcgag aacgttccgg cgccggagaa tcccaagact tcctggctcg cgtgctacag
    20881 cgcgctcgcc gccttgcgcg ctctcgaatc caaggtccgg tacggaactt gaaatcctct
    20941 ccgcttttct tgaccaagcg taggaaaact cacataattt cgggttccgc cggaggggtg
    21001 cccgagcggc taaagggggc agactgtaaa tctgttggcc ttgcgcctac gttggttcga
    21061 atccaacccc ctccaccagc tagctagggc actcaacagg cgacgggatt ttcgggtgaa
    21121 gaacttttgt gtcgagcagt tgagcagtca ggctaaaagg caggcgggtg tagctcaatg
    21181 gtagagcaga agccttccaa gcttatgacg agggttcgat tcccttcacc cgctccagac
    21241 aacgcgcgca ggttccgaac tttcgcccat gtagctcagt ggtagagcac tcccttggta
    21301 agggagaggt cacgcgttca atccgcgtca tgggcaccag gtgatcagga aaccgtagca
    21361 ctaggaaatc gagatggcca aaggcaaatt cgagcgcacc aagccgcacg ttaacgtggg
    21421 gacgatcggg cacgtggatc acggcaagac gacgctgacg gcggcgatga cgcacattct
    21481 ggcgaagaag tacgggggcg aggcgaagaa ctac
//
LOCUS       DATBSZ010000222         8141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_996258, whole genome shotgun sequence.
ACCESSION   DATBSZ010000222 DATBSZ010000000
VERSION     DATBSZ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996258"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VIG70_13910"
     CDS             <1..588
                     /locus_tag="VIG70_13910"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY8251694.1"
                     /translation="CRALGIAPLVRVPTTEYHFVARALDAGALGVMVPLVDTAEQAER
                     IVSFTRYPPKGRRGAAFGFAHDDYQGGDVIEKMRAIHERTLVICMIETRAGLENVDAI
                     AAVDGVDVIWLGHFDLTNFLGIPGQFSHPQYRDAVKRIAAAAKKHGKAAGYMAASPDL
                     GQEYLGHGFRMIATGTDQGLLQNATAQLISTWKVN"
     gene            590..1606
                     /locus_tag="VIG70_13915"
     CDS             590..1606
                     /locus_tag="VIG70_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8251695.1"
                     /translation="MPGTFRVGVTSDILDSRGEPAFGREPLQALDQLKWEWLPRGLKE
                     ITAEHAAAYDALYVNSPRVPASAVAGPDLRLKLISRHGVGYDSVDVAAMTQAGVLVAN
                     TPNAVPRPVATIALTFILALAHRLMAKDRITREGNWPARLDLMGTGLTGRTLGVIGAG
                     RIGKELLRMAKVFDLRLLAADPYAERLELEYIGARHVPLDELMRESDFVVVACLLNDE
                     TRRLVGARQLALMKPTAYFINVARGPIVDEKALYETLAARRIAGAGLDVFEQEPTPAD
                     NPILKLDNVLVSPHALCWTDELFGNIARTAIGAILAVHAGRRPEFAVNPVALSHPRAK
                     AWLK"
     gene            1643..2950
                     /locus_tag="VIG70_13920"
     CDS             1643..2950
                     /locus_tag="VIG70_13920"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8251696.1"
                     /translation="MARITRRTFLQYSGAGMAGILASHRAPVFAQANTVHILRWNDFV
                     PAADKVLREVFAPEVQKALGLKLNVETVNANDIPARATSSIQSGNGPDIIMILNNYPH
                     LYSSAAVDVSAVAEEVAKAQGGIYDAATQLNRIGGKWVSMPWSMVPALIAYRKSWFEE
                     AGAKSFPKTWQEYHAVGKKLKAAKRPIGQTLGNTFGDAPTFTYPFLWSFGGQEVDAKG
                     KVVLDSKETLESVRFMTQFWKDAHDEGGLAWDDSNNNRAFLSGDISASLNGASIYVAA
                     LNGADKFKTDKGAPLHTDILHAALPAGPKGAHAYHTAFNHMVMKYSKNAKGAIEFLKW
                     AHKKENYEKWFTVQKGFAVGPTKEWENHAMWKNDPVMAPFRTAPQAQKRLMGYQGQPD
                     KRAAEAWNKFIVIVMYARACGGTKPEEAVKWAAGELGKIYTGT"
     gene            2969..3898
                     /locus_tag="VIG70_13925"
     CDS             2969..3898
                     /locus_tag="VIG70_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8251697.1"
                     /translation="MNETLVVNGRRQRVSRLTRLLESEKALAGALLAPGVAILAIFIA
                     YPFVMALWFSLTSIRVGEAGEFVGLANFTKAINDTIFQMAFKNTVFYTFWATIFKLAL
                     GMWLALLLNRHFRFKRIVRASMLLPFIIPTVLSAFAWRWMFDPTFSVMNWGLYHAGMI
                     TDRLPFLSDGDWAMWCAVAVNTWRGMPFFAITLLAGLQTISPDLHEAAALDGANAWHR
                     FWHVTWPLLLPVTLVVVVFSVIQTFSDFQLIYVLTGGGPANSTHLIATYAYQIGVGSG
                     LLGEGAALSLFMFPVLFAVVWVQLRSLRRMENN"
     gene            3911..4762
                     /locus_tag="VIG70_13930"
     CDS             3911..4762
                     /locus_tag="VIG70_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8251698.1"
                     /translation="MLIERKWKKWVYFYLPMAVFIVFTLFPFYWMAVTAFRPDPELYA
                     TWRQPNAAPLWTLHPTLEHVKGLLQTTAFPTWLWNTMLIAIVSTIISLICGMFAGYAL
                     ARLRFRGSEFLGTAIFITYLVPQTLLFIPLADIIRDMQLGNTPWALMLTYPTILIPFC
                     TWLLMGYFKTIPKELEECARIDGATRFGAMVRIIFPVAIPGILSAGIFAFTLSWNEFI
                     YALVFMASADQKTVPVGVVSELIRGDIFFWGQLMAGALLGSVPVALVYSFFVEYYVTG
                     LTGSVKG"
     gene            4845..5939
                     /gene="ugpC"
                     /locus_tag="VIG70_13935"
     CDS             4845..5939
                     /gene="ugpC"
                     /locus_tag="VIG70_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY8251699.1"
                     /translation="MADVSIRALNKKYDNGFHAVKDVNLEIRDKEFLVLVGPSGCGKT
                     TTLRMVAGLEEITSGEIRIGEEVVNDLPPMDRNIAMVFQNYALYPHKTVFDNMAFGLQ
                     MRGYAKDDILKRVREAAEILGIEPLLERKPRQLSGGQRQRVAVGRAIVRHPEVFLFDE
                     PLSNLDAKLRVQMRVELKRLHERLETTAIYVTHDQVEAMTLGDRVVVMKDGRVQQIGE
                     PLELYGRPANRFVAGFIGSPAMNFVEVAISGAGDALWAEAEGLRLRVPRDLSSRIRTG
                     TRLTLGVRPEALRLANGTEAGSDGHTFATAVDVIEPLGNEILLNFRAGGVPMVARVDP
                     GVRVKAHESIQLALDPQRLHFFDAKTEAAI"
     gene            5947..6771
                     /locus_tag="VIG70_13940"
     CDS             5947..6771
                     /locus_tag="VIG70_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8251700.1"
                     /translation="MADAHFHVWDPRAHYYPWLCDPQPIAFRYGDYSALRRPYLVADY
                     RADSQGWDVRRGVYVEAEWDPRAPLGEMAFISRIREGASFPTVAIAQAWLHHDDCAQV
                     LEGHAQHDFVRGIRHKPKPGMMDDAKWRAGYRHLAPLGLHFELQAPWSELEEAARLAG
                     DFPETTIVLNHTGLPYVEQLGGWRSAMAALAVRPNVALKISGLGNVEHKREVVLAAIE
                     LFGTGRCMFASNFPVDSLRESFDSIFSGFDEFTRGFSDAERRALFHDNAVRIYRME"
     gene            6774..7616
                     /locus_tag="VIG70_13945"
     CDS             6774..7616
                     /locus_tag="VIG70_13945"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8251701.1"
                     /translation="MKKFSLGYVGVGLMGGPMVKRLTALGWSVRGYDIVPERSTVGSV
                     AEAAQGADVVLLNLPTNEAVEDAVFGAKGLIHAMKKPQLLVDFSTIPVDACRSHAARL
                     AKATGCRWVDAPVSGGPPAVEAGTLTVMAGGEADDVARLAPLMKDIAGRCTRMGPVGS
                     GLAAKMINQLIVGVGHAMLGEAVGLCEKAGIDAARIPECLAGGYADSNVMKAYWPRMV
                     ERDFAPRGYVRQLLKDLEMVSAWAEGLHSKTPVLQSALAAYREMKKRGLTELDTSAVV
                     KLYL"
     gene            complement(7607..>8141)
                     /locus_tag="VIG70_13950"
     CDS             complement(7607..>8141)
                     /locus_tag="VIG70_13950"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY8251702.1"
                     /translation="TEFNVLEGLWNARVTSHIPLAEVSASLSIEKIVAALGLTATALR
                     AAGFNPPRIGVAGLNPHAGDGGNFGREEIDIIEPAVRKAKAQGLPADGPYPADTVFVR
                     AKAGQFDAVLTMYHDQGQIAMKLLGFDRGVTLLGGYPFPICTPAHGTAYDIAGKGVAN
                     VGAARAALLLAARMSQR"
BASE COUNT         1374 a         2716 c         2702 g         1349 t
ORIGIN      
        1 tgccgcgcgc tcggcatcgc accgctggtg cgcgtgccca ccaccgagta ccacttcgtc
       61 gcgcgcgcgc tcgacgcggg agcgctcggc gtcatggtgc cgctggtgga cacggccgag
      121 caggccgagc gcatcgtgtc gttcacacgc tacccgccga agggacgccg cggcgccgcc
      181 ttcggtttcg cgcacgacga ctaccaaggc ggagacgtca tcgagaaaat gcgcgccatc
      241 catgagcgca cgctggtgat ctgcatgatc gagacgcgcg ccggcctgga gaacgtcgac
      301 gcgatcgcgg cggtcgacgg cgtggacgtg atctggctcg ggcacttcga cctgaccaat
      361 ttcctcggca tccccgggca gttctcgcac ccgcaatacc gcgacgcggt gaagcgcatc
      421 gccgccgccg cgaagaagca cggcaaggcg gcgggctata tggcagcaag cccggacctc
      481 ggacaggagt acctcggcca cggcttccgc atgatcgcca ccggcaccga ccagggcttg
      541 ctgcagaacg caaccgcgca attgatctcc acgtggaaag tgaattgaga tgccgggcac
      601 cttccgcgta ggagtcacca gcgacatcct cgactcgcgc ggcgagcccg cgttcggccg
      661 cgagccgttg caggctctcg accagctcaa gtgggaatgg ctgccgcgcg gcctgaagga
      721 aatcaccgcc gagcacgccg ccgcttacga cgcgctctat gtgaactcgc cgcgagtccc
      781 ggcatccgcc gtcgcggggc ccgaccttcg cctgaagctg atctcgcgcc acggcgtcgg
      841 ctatgacagc gtcgacgtgg ccgcgatgac gcaagcaggc gtgctggtcg ccaacacgcc
      901 gaacgcggtg ccgcggccgg tggcgaccat cgcgctcacc ttcatcctcg ccctcgcgca
      961 ccggctgatg gccaaagacc ggatcacgcg cgaaggcaac tggccggcgc gcctcgacct
     1021 gatggggacc ggcctcaccg ggcgcacgct gggcgtgatc ggcgcgggcc ggatcgggaa
     1081 agagctgctg cgcatggcca aggtcttcga cctgaggctg ctggccgccg acccctacgc
     1141 cgagcgcctg gagctcgagt acatcggagc gcgccacgtg ccgctcgacg agctcatgcg
     1201 cgagtcggac tttgtcgtcg tcgcctgcct gctgaacgat gagacgcgac gcctggtcgg
     1261 cgcccggcag ctcgccctga tgaagcccac cgcctacttc atcaatgtcg cgcgcggccc
     1321 gatcgtggac gaaaaggcgc tctacgaaac gctggccgcc cggcgcatcg ccggcgccgg
     1381 gctcgacgtg ttcgagcagg agcccacccc cgcggacaat cccatcctga agctcgacaa
     1441 cgtgctcgtc agcccgcacg cgctgtgctg gaccgacgag ctgttcggca acattgcccg
     1501 cactgcgatc ggcgcaatac tggcggtgca cgcgggccgg cgccccgagt tcgcggtcaa
     1561 cccggtggcg ctttcgcatc cgcgcgccaa ggcatggcta aagtagtaga gttctccaca
     1621 ccaccttaga ggagggggaa acatggctcg aatcacgcga cgcacgttcc tgcagtactc
     1681 cggcgccgga atggcgggca ttctcgcttc gcaccgcgcg ccggtctttg cacaagcgaa
     1741 taccgtgcac atcctgcgct ggaacgattt cgttcccgcg gcggacaagg tgctgcgcga
     1801 ggtgttcgcg ccggaagtac agaaggcgct cggcctcaag ttgaatgtgg aaaccgtcaa
     1861 cgccaacgac atcccggcgc gcgcgacctc gtcgatccag tcgggcaacg gtcccgacat
     1921 catcatgatc ctgaacaact acccccacct ctactccagc gcggcggtgg acgtgagcgc
     1981 ggtggccgaa gaggtggcca aggcgcaggg cgggatctac gacgccgcga cgcagctcaa
     2041 ccgcatcggc ggcaagtggg tatcgatgcc ctggtcgatg gtgccggcgc tcatcgcgta
     2101 ccggaaatcc tggttcgagg aagccggcgc caagagcttc ccgaagacct ggcaggagta
     2161 tcacgcggtc ggcaagaagc tgaaggcggc caagcgcccg atcggccaga cgctcggcaa
     2221 caccttcggc gacgcgccca cgttcaccta tccgttcctg tggtccttcg gcggccagga
     2281 agtggacgcc aagggcaagg tggtgctcga ctcgaaggag accctggagt cggtgcgctt
     2341 catgacccag ttctggaagg acgcgcacga cgagggcggc ctcgcctggg acgactcgaa
     2401 caacaaccgg gcgttcctgt cgggcgacat cagcgcctcg ctcaacgggg cgtcgatcta
     2461 cgtcgcggcg ctcaacggcg cggacaagtt caagaccgac aagggcgcgc cgctgcacac
     2521 cgacatcctg cacgcggcgc tgcccgcggg cccgaagggc gcgcacgcct accacaccgc
     2581 gttcaaccac atggtgatga agtattcgaa gaacgccaag ggcgcgatcg agttcctgaa
     2641 gtgggcgcac aagaaggaga actacgagaa gtggttcacc gtgcagaagg gcttcgccgt
     2701 cggcccgacg aaagagtggg aaaaccacgc catgtggaag aacgacccgg tgatggcgcc
     2761 gttccgcacc gcgccgcagg cgcagaagcg gctgatgggc taccagggcc agcccgacaa
     2821 gcgcgccgcc gaggcgtgga acaagttcat cgtcatcgtg atgtacgcgc gggcctgcgg
     2881 cggcaccaag cccgaggaag cggtgaagtg ggcggcgggt gagctgggca aaatctacac
     2941 tggaacctga gcgtcacctg atccgcgctt gaacgaaacg ctcgtcgtca acgggaggcg
     3001 ccagcgcgtc tcccgtctga cgcgcctact cgaaagcgaa aaggcgctgg caggtgccct
     3061 gctggcgccc ggggtggcga tcctcgccat cttcatcgcg tatccgttcg tgatggcgct
     3121 gtggttctcg ctcaccagca tccgggtggg cgaggccggc gagttcgtcg gcctggccaa
     3181 cttcacgaag gcgatcaacg acaccatctt ccagatggcg ttcaagaaca ccgtgttcta
     3241 cacgttctgg gcgacgatct tcaagctcgc gctcggcatg tggctcgcgc tgctgctcaa
     3301 ccggcatttc cgcttcaagc gcatcgtgcg cgcctcgatg ctgctgccgt tcatcatccc
     3361 gacggtgctc tcggcgttcg cctggcgctg gatgttcgac ccgaccttca gcgtgatgaa
     3421 ctgggggctg tatcacgcgg gcatgatcac cgaccggctg ccgttcctgt cggacggcga
     3481 ctgggcgatg tggtgcgcgg tcgcggtgaa cacctggcgc ggcatgccgt tcttcgccat
     3541 caccctgctc gccgggctgc agaccatcag tccggacctg cacgaggccg cggcgctcga
     3601 cggcgccaac gcctggcacc gcttctggca tgtcacctgg ccgctgctgc tgccggtgac
     3661 gctggtggtg gtggtgttct ccgtgatcca gaccttctcg gacttccagc tgatctacgt
     3721 gctcaccggg ggcgggccgg cgaactcgac gcacctcatc gccacctacg cctaccagat
     3781 cggcgtcggc tcgggcctgc tcggcgaagg cgcggcgctg tcgctcttca tgttcccggt
     3841 gctgttcgcg gtcgtctggg tgcagctgcg ctcgctccgg cgcatggaaa ataactgaga
     3901 tgcgcgccta atgctcatcg agcgcaagtg gaagaagtgg gtgtacttct acctgccgat
     3961 ggcggtgttc atcgtgttca cgctgttccc gttctactgg atggcggtga ccgcgttccg
     4021 gccggacccc gagctctatg ccacctggcg ccagccgaac gccgctccgc tgtggacgct
     4081 gcacccgacg ctcgagcatg tgaagggcct gctgcagacc acggcgtttc cgacctggct
     4141 gtggaacacc atgctgatcg ccatcgtctc gacgatcatc tcgctgatct gcggcatgtt
     4201 cgcgggctac gcgctcgcgc gcctgcgctt ccgcggctcg gagttcctcg gcaccgcgat
     4261 cttcatcacc tacctggtgc cgcagacgct gctcttcatc ccgctcgccg acatcatccg
     4321 cgacatgcag ctcggcaaca cgccgtgggc gctgatgctc acctacccga cgatcctgat
     4381 cccgttctgc acctggctct tgatgggcta cttcaagacc atcccgaagg agctcgagga
     4441 gtgcgcgcgc atcgacggcg cgacgcgctt cggcgcgatg gtgcgcatca tctttccggt
     4501 ggcgatcccc ggcatcctct cggccgggat cttcgcgttc acgttgtcgt ggaacgaatt
     4561 catctacgcg ctcgtcttca tggcctcggc cgaccagaag accgtcccgg tcggcgtggt
     4621 gtcggagctg atccggggcg acatcttctt ctggggccag ctcatggcgg gagcgctgct
     4681 cgggtccgtg ccggtggcgc tcgtgtattc gttctttgtc gagtattacg tcaccggcct
     4741 cacgggatct gtgaaaggct gaccacttga aaccagggac agaccacgtt tttcctttat
     4801 caaccacggc cgaaaaacgt ggtctgtccc tgattttctg agccatggct gacgtttcaa
     4861 tccgcgcgct gaacaagaag tacgacaacg ggtttcacgc ggtgaaggac gtgaacctgg
     4921 agatccgcga caaggagttc ctggtgctgg ttggtccgtc cggctgcggc aagaccacga
     4981 cgctgcgcat ggtggccggc ctcgaggaga tcacctcggg cgagatccgc atcggcgagg
     5041 aggtcgtcaa cgacctgccg ccgatggacc gcaacatcgc gatggtgttc cagaactacg
     5101 cgctctatcc gcacaagacc gtgttcgaca acatggcctt cggcctgcag atgcgcggct
     5161 atgcgaagga cgacatcctc aagcgcgtgc gcgaggccgc cgaaatcctg gggatcgaac
     5221 cgctgctgga acgcaagccg cgccagctct ccgggggcca gcgccagcgc gtcgcggtcg
     5281 ggcgcgcgat cgtgcgccat cccgaggtgt tcctgtttga cgagccgctg tcgaacctgg
     5341 acgcgaagct gcgcgtgcag atgcgcgtcg agctcaagcg cctgcacgag cgcctcgaga
     5401 ccacggcgat ctacgtcacc cacgaccagg tcgaggcaat gaccctcggc gaccgcgtgg
     5461 tggtgatgaa ggacggccgc gtgcagcaga tcggcgagcc gctcgagctc tacggccggc
     5521 cggccaaccg gttcgtcgcc ggcttcatcg gctcgccggc gatgaacttc gtcgaggtcg
     5581 cgatcagcgg cgccggcgac gcgctctggg cggaagccga gggcttgcgc ctccgggttc
     5641 cccgggatct ctcttcgaga atacgaacag gaacgcgcct cactctcggc gtgcggccgg
     5701 aggcgctgcg cctcgccaat ggcacagagg cgggctccga cgggcacacg ttcgccacgg
     5761 cggtcgacgt gatcgagccg ctcggcaacg agatcctgct caacttccgc gccggcggcg
     5821 tgccgatggt ggcgcgcgtg gatcccggcg tgcgcgtgaa ggcgcacgag agcatccagc
     5881 tcgcgctcga cccgcagcgc ctgcacttct tcgacgccaa gactgaagcc gcaatctaga
     5941 tttccgatcg cggacgcgca cttccacgtc tgggacccgc gggcgcatta ctacccctgg
     6001 ctgtgcgatc cgcaaccgat cgcgttccgc tatggcgact acagtgcctt gagacggcct
     6061 tacctcgttg ccgactaccg ggccgactcc cagggctggg acgtccggcg cggcgtctac
     6121 gtggaagccg agtgggatcc gcgcgctcct ctgggcgaga tggcgtttat ttcgagaatc
     6181 agagaaggag cgagcttccc gacggtcgcc atcgcgcaag cctggctgca ccacgatgac
     6241 tgcgcccagg tgctcgaagg gcacgcgcag cacgatttcg tgcgcggcat ccggcacaag
     6301 cccaagcccg gcatgatgga cgacgccaaa tggcgcgcgg ggtaccggca cctcgcgccg
     6361 ctcgggctgc atttcgagct gcaggcgccg tggagcgagc tcgaggaggc ggcgcgcctc
     6421 gcgggagact tcccggaaac cacgatcgtg ctgaaccaca ccggcttgcc ctacgtagag
     6481 cagcttggcg gctggcgcag tgccatggcc gccctggccg tacgcccgaa cgtggccctg
     6541 aaaatctcag gcttgggcaa tgtggagcac aagcgcgagg tggtgctcgc cgcaatcgag
     6601 ctgttcggca ccgggcgctg catgttcgcc agcaacttcc cggtcgacag cctgcgcgag
     6661 agcttcgatt cgatcttttc gggcttcgac gagttcacgc gcggcttctc cgatgcggag
     6721 cgccgcgccc tgttccacga caatgccgtt cgcatctatc gcatggagtg aggatgaaga
     6781 aattttctct gggctacgtt ggcgtcgggc tcatgggcgg cccgatggtg aagcgcctga
     6841 ccgcgctcgg ctggagcgtg cgcggctacg acatcgtccc cgaacgctcg acggtcggct
     6901 ccgtcgcgga agcagcgcag ggcgcggacg tggtgctgct caacctgccg acgaacgagg
     6961 cggtcgagga cgcggtgttc ggcgccaagg ggctgatcca cgccatgaag aaaccgcagc
     7021 tgctggtcga cttctcgacg attccggtcg atgcctgccg cagccacgcg gcgcgcctgg
     7081 cgaaggcgac cggctgtcgc tgggtcgatg cgccggtttc cggcggcccg ccggcggtgg
     7141 aagccggcac gcttacggtg atggccggcg gagaagcgga cgacgtggcg cgcctcgcgc
     7201 cgctgatgaa ggacatcgcc gggcgatgca cgcgcatggg tccggtcggc tccggcctcg
     7261 ccgcgaagat gatcaaccag ctcatcgtcg gcgtcggtca cgccatgctc ggcgaagcgg
     7321 tcgggctctg cgagaaggcg gggatcgacg ccgcccgcat ccccgagtgc ctcgccggcg
     7381 gctacgccga cagcaacgtg atgaaggcct actggccgcg catggtcgag cgcgacttcg
     7441 cgccgcgcgg ctacgtacga cagctcctga aagacctcga gatggtcagc gcctgggccg
     7501 aaggtcttca ttcgaaaacc ccggtcctgc agagcgcgct tgcggcctat cgcgagatga
     7561 agaagcgcgg gttgaccgag ctcgatacct cggccgtggt gaagctttat ctttgagaca
     7621 tgcgggcggc gagcagcagc gctgcccgcg ccgcgccgac gttggcaacg cctttgccgg
     7681 cgatgtcata ggcggtgccg tgcgccgggg tgcagatggg gaacgggtag ccgccgagca
     7741 gcgtgacgcc gcggtcgaag ccgagcagct tcatcgcgat ctgtccctgg tcgtggtaca
     7801 tggtcagcac cgcgtcgaac tgcccggcct tggcgcgcac gaacaccgtg tcggccggat
     7861 aagggccgtc ggcgggcaac ccctgcgcct tcgccttgcg caccgcgggc tcgatgatgt
     7921 cgatctcctc gcggccgaag ttgccgccgt cgccggcgtg cggattgagc ccggccacgc
     7981 cgatacgggg tggattgaat ccggccgcgc gcagcgccgt ggcggtaagc ccgagcgcgg
     8041 cgacgatctt ctcgatggag agactggctg aaacctcggc gagcgggatg tgcgaggtga
     8101 cgcgcgcgtt ccacagcccc tcgagcacgt tgaactcggt g
//
LOCUS       DATBSZ010000223         6046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1065260, whole genome shotgun sequence.
ACCESSION   DATBSZ010000223 DATBSZ010000000
VERSION     DATBSZ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065260"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VIG70_13955"
     CDS             <1..975
                     /locus_tag="VIG70_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HEY8251703.1"
                     /translation="AKLNFLGIVFCMMFGTAALPHILMRYYTTPSVRQARTSVFWSLF
                     FIFMLYFTAPCLAVLAKYDVYSHLVGSRFAELPAWAAAWRLVDPALLTITDLNFDGIV
                     QLAEIAIGGDIIVLATPEIAGLPYVVSGLVAAGGLAAALSTADGLLLTIANALSHDLY
                     YKMLDPNASTARRVTTSKILLLVVAIIAAWVTSYKPGNILFLVGAAFSLAASAFFPAL
                     VLGIFWKRANKWGAIIGMAAGLGVCMYYMSVTYPFLRDLLGVTAPIASYQWFGMNAIS
                     AGVFGVPVGLATIIIVSLLTPAPDRKVQELVEHVRYPNLAGDIDTRAT"
     gene            1023..1355
                     /locus_tag="VIG70_13960"
     CDS             1023..1355
                     /locus_tag="VIG70_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251704.1"
                     /translation="MQTELLLVVIVKALAELAGMFLLGRGLLWVLAGGKRMDNIFFQV
                     IAVLTNPVIRAARWIMPRVVMDSYIPAIAFGMVAWIWLAIVFWVLPEMCSSGSYDCST
                     LMQRKLAD"
     gene            complement(1352..1924)
                     /locus_tag="VIG70_13965"
     CDS             complement(1352..1924)
                     /locus_tag="VIG70_13965"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8251705.1"
                     /translation="MSPRLLFLFAQACIFFKQHARALAALETLLREQPDHPRAWSIAG
                     FLYAEKGRHVDAIAAFERALALAPDDAPTLFNLGFVLQKAGRHEDAIARMGRAIKLSP
                     LLDRAWYGLGLSLIHCGRFREAIERLTEAARLQPLNPYARYQLAAAWFKLGEPEKVRA
                     EYRKLKSFDPTVAAHVRTDFGVPKDGDEVG"
     gene            complement(1921..2289)
                     /locus_tag="VIG70_13970"
     CDS             complement(1921..2289)
                     /locus_tag="VIG70_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251706.1"
                     /translation="MEPADSIARLGFARWYERRLIEAHAWFVSAFVCVVAIAACAEEL
                     SFRGSAARLLFYVAVILGAAATGIYGMMRYHKILVEAERIGEHATCEGCGAYARFKLI
                     SASNGRCRKCNHEWRVIVPE"
     gene            2336..2426
                     /locus_tag="VIG70_13975"
     tRNA            2336..2426
                     /locus_tag="VIG70_13975"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2370..2372,aa:Ser,seq:gga)
     gene            2457..3245
                     /locus_tag="VIG70_13980"
     CDS             2457..3245
                     /locus_tag="VIG70_13980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8251707.1"
                     /translation="MRIYRLTAAGREAWEKQDAAVPADYRRILWMMDLQGQGGVTENL
                     SKLYPPEMLDEWLSEMEEIGLIEELPEGATHPGWLTPRDRDKTLALDQAQLREAAKIA
                     AGSLARTGAYLALDRLRRRPLPIKAPADMVVLIVEDDPDQLALADLRVSMAGYKVRVA
                     SSVNGFLQDLVDQGAPDLLLLDVMLPDGDGFDVLGKMRRHEALGSLPIVLLTARNDPA
                     DIGRGLALGADGYITKPYTKNILADVIGRVLKREQDGIEKRPPG"
     gene            3392..3490
                     /gene="ffs"
                     /locus_tag="VIG70_13985"
     ncRNA           3392..3490
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VIG70_13985"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3507..5015
                     /gene="dnaX"
                     /locus_tag="VIG70_13990"
     CDS             3507..5015
                     /gene="dnaX"
                     /locus_tag="VIG70_13990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY8251708.1"
                     /translation="MSYQVLARKWRPRDFASLVGQEHVVRALRHALENKRLHHAYLFT
                     GTRGVGKTTLARILAKCLNCETGITPQPCNTCSACVEIDAGRFVDLLEVDAATNTKVD
                     EMRQLLETAQYAPTRGRFKVYVIDEVHQLSGHAFNAMLKTLEEPPEHMKFILATTEPD
                     KIPVTILSRCLQFNLKQMPREAITEHLQGVLGKEGIQHEPEALSLLARAAAGSMRDAL
                     SLLDQAIAHGGGSVGAASVGDMLGTIDQGHLVRLLEAVAAGDAAGAVQIADEMQARSL
                     SFDTALADLASLLLQVALAQSLADAVDDARARELATRIDAESVQLYYQIALQGREDLP
                     LAPDEHAGFVMTVLRLLAFRPEGAPKGVALPKAAKPAPQNSKPTGWPELVQQLAVTGA
                     ARELARNAELKQRQASSFELVVPKAKAYLAERSYVDKLKAALEQHLGGAVSLKVSAGE
                     VAGGSVASLEAGEREARRAAAAQAVKSDGFVQDLVNMFDGKIVDSTIRPEGK"
     gene            5023..5346
                     /locus_tag="VIG70_13995"
     CDS             5023..5346
                     /locus_tag="VIG70_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY8251709.1"
                     /translation="MKGNIGQLMKQAQQMQENMRRMQESLAVTEVEGQSGAGMVKVQM
                     TCKYEVKRVTIDPSLVGDDREMLEDLVVAAFNDAARKAEATIAEKMGGMTAGLGLPPG
                     FKLPF"
     gene            5343..5954
                     /gene="recR"
                     /locus_tag="VIG70_14000"
     CDS             5343..5954
                     /gene="recR"
                     /locus_tag="VIG70_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY8251710.1"
                     /translation="MSKTPVPTAKGLERLVQALRVLPGVGPRSAQRMAFHLLQHERGG
                     AEELARALAEALASVQRCAQCNNFSEDEVCALCRSPRRDRSLLCVVETPADLAMVEQT
                     LSYSGMYFVLMGRLSPLDGVGPRDIGLDRLLARAAEGEVQEVILATNFTNEGEATAHY
                     IAEMLRARSVRATRIARGVPLGGELEYVDAGTISQALLDRRGI"
BASE COUNT          990 a         2012 c         2094 g          950 t
ORIGIN      
        1 gccaagctca acttcctcgg catcgtgttc tgcatgatgt tcggcaccgc ggcgctgccg
       61 cacatcctga tgcgctacta caccacgccc agcgtgaggc aggcgcgcac ctcggtgttc
      121 tggtcgctgt tcttcatctt catgctgtac ttcaccgcgc cgtgcctcgc ggtgctcgcc
      181 aagtacgacg tgtactcgca cctggtgggc agccgatttg cggagcttcc ggcatgggcg
      241 gcggcctggc gactggtaga tccggcgcta ttgacgatca ccgacctcaa cttcgacggc
      301 atcgtgcagc tcgcggagat cgccatcggc ggcgacatca tcgtgctggc gacgcccgag
      361 atcgcgggcc tgccgtacgt ggtgtcgggc ctggtcgccg caggcggcct ggcggcggcg
      421 ctgtcgaccg cggacggcct gctgctgacg atcgccaacg ccctgtcgca cgacctgtac
      481 tacaagatgc tcgatccgaa cgcgtccacg gcgcgccgcg tcacgacctc gaagatcctg
      541 ctgctggtcg tcgcgatcat cgccgcctgg gtgacctcct acaagccggg gaacatcctg
      601 ttcctggtcg gcgcggcgtt ctcgctcgcg gcgtccgcct tcttcccggc gctggtgctg
      661 gggatcttct ggaagcgggc caacaagtgg ggtgcgatca tcggcatggc ggcggggctc
      721 ggcgtctgca tgtactacat gagcgtcacc tatccgttcc tgcgcgacct gctcggggtg
      781 acggcgccca tcgcttccta ccagtggttc ggcatgaacg ccatctcggc gggcgtgttc
      841 ggcgtgccgg tgggcctggc gacgatcatc atcgtgagcc tgctcacccc cgcgccggac
      901 cgcaaggtcc aggagctggt ggagcacgtg cgctacccga acctggcggg cgacatcgac
      961 acccgcgcca cctgaggctg cttcaggcac gataaagccc gcgcaagcgg gctttatttt
     1021 ttatgcagac tgaattgctg ctggtagtga tcgtcaaggc gctggcggag ctcgccggca
     1081 tgttcctgct cgggcgcggg ctgctctggg tgctcgccgg cggtaagcgg atggacaaca
     1141 tcttcttcca ggtgatcgcc gtcctgacca atcccgtgat ccgcgcggcg cgctggatca
     1201 tgccgcgcgt ggtcatggac agctacatac cggcgatcgc gttcggcatg gtggcctgga
     1261 tctggctggc gatcgtgttc tgggtactgc ccgagatgtg ctcatccggc agctacgact
     1321 gctcgacgct gatgcagcgc aagctcgccg attagcccac ctcgtcgccg tccttcggca
     1381 cgccgaaatc ggtgcgcacg tgcgcggcga cggtcgggtc gaagctcttc agcttgcggt
     1441 actccgcgcg cactttttcc ggctcgccga gcttgaacca ggccgccgcg agctggtagc
     1501 gcgcgtaggg gttgagcggc tgcagccgcg cggcctcggt caggcgctcg attgcctcgc
     1561 ggaaacggcc gcagtggatg agcgacaaac cgaggccgta ccaggcgcgg tcgagcagcg
     1621 ggctgagctt gatcgcgcgt cccatgcggg cgatcgcgtc ttcgtgccgg ccggccttct
     1681 gcaggacgaa gccgaggttg aacagcgtgg gcgcgtcgtc gggcgcgagg gcgagggcgc
     1741 gctcgaacgc ggcgatcgcg tccacgtggc ggcccttctc ggcgtacagg aagccggcga
     1801 tgctccaggc gcgcggatga tcgggctgct cgcgcagcag cgtctcgagc gccgccagcg
     1861 cgcgcgcgtg ctgcttgaag aagatgcagg cctgcgcgaa caggaacagc aggcgcgggc
     1921 tcattcgggc acgatgacgc gccattcgtg gttgcacttg cggcagcgtc catttgatgc
     1981 cgagatcagc ttgaagcggg cgtaggcgcc gcaaccctcg caggtggcgt gctcgccgat
     2041 gcgctcggcc tcgaccagga tcttgtggta gcgcatcatt ccgtagatcc cggtcgccgc
     2101 ggcgcccagg atgacggcga cgtagaacag cagtcgtgcc gcggaaccgc ggaaggagag
     2161 ctcttccgcg caggcggcga tggccactac gcagacgaat gcgctgacaa accaggcgtg
     2221 agcctcgatc agccggcgct cgtaccagcg ggcgaagccg agtcgggcga tgctgtcggc
     2281 cggctccatg actttgcgat ggtactccgg cggtaccgcg ctagaatccc ctgacggaga
     2341 gatggccgag cggcttaagg cgcacgcttg gaaagcgtgt gtacgttaat agcgtaccgt
     2401 gggttcgaat cccactctct ccgccacaaa cagaacaggc tcccggggca aaggccgtgc
     2461 gcatctaccg attgaccgcc gccgggcgcg aagcctggga gaagcaggac gcagccgtgc
     2521 ccgccgacta ccggcgcatc ctgtggatga tggacctcca ggggcagggc ggcgtcacgg
     2581 aaaatttgtc gaagctctat ccgccggaaa tgctcgacga atggctttcc gagatggaag
     2641 agatcgggct catcgaggag ctgccggaag gggcgaccca tccgggctgg ctcacgcccc
     2701 gcgacaggga caagacgctc gcgctcgacc aggcgcagct gcgcgaggcc gcgaagatcg
     2761 cggccggttc gctggcgcgc accggcgcct atctcgcgct cgaccggctg aggcgccgcc
     2821 cgctgccgat caaggcgccg gccgacatgg tggtcctgat cgtcgaggac gatcccgacc
     2881 agctcgcgct cgccgacctg cgcgtctcga tggccgggta caaagtgcgc gtggcgagct
     2941 cggtcaacgg cttcctgcag gacctggtgg accagggtgc gccggacctg ctgctcctcg
     3001 acgtcatgct gcccgacggc gacggcttcg acgtcctggg caagatgcgc cggcatgagg
     3061 cgctcggctc gctgccgatc gtgctgctga ccgcgcgcaa cgaccccgcg gacatcgggc
     3121 gaggtcttgc cctcggcgcg gacggctaca tcaccaagcc ctataccaag aacatcctgg
     3181 cggacgtcat cgggcgcgtg ctgaagcgcg agcaggacgg catagaaaaa cgcccgccgg
     3241 gctagtgccc gccgggcgtt ggaaccatcg cgcttacgag gacgcgatgg aggaggatac
     3301 gtcgcaagag tagcgccgcc gaccgacgac ggcctgactc caagctgacg gcgggcttac
     3361 tgcgccggtc gtttcgaata caattgcaga cggcgggcct ccccgcattg cagtctggtg
     3421 aacctggtca ggtccggaag gaagcagcca cagccggtcg ctgcgagtgc cgggggtcag
     3481 gctcgccacc gaattcgact cgagcgatga gctatcaagt ccttgcccgc aaatggcggc
     3541 cgcgcgattt cgccagcctc gtgggccagg agcacgtggt tcgcgcgctg cgccacgcgc
     3601 tcgagaacaa gcgcctgcac cacgcgtacc tctttaccgg gacgcgcggc gtcggcaaga
     3661 ccaccctggc gcgcatcctc gcgaagtgcc tgaactgcga aaccggcatc acgccgcagc
     3721 cgtgcaacac ctgctcggcg tgcgtcgaga tcgacgcggg acgtttcgtc gacctgctgg
     3781 aagtcgacgc cgcgaccaac accaaggtcg acgagatgcg ccagctcctc gagaccgcgc
     3841 agtacgcgcc gacgcgcggg cggttcaagg tctacgtgat cgacgaggtg caccagctct
     3901 cgggccacgc cttcaacgcg atgctgaaga cgctggagga gccgcccgag cacatgaagt
     3961 tcatcctggc gacgaccgag cccgacaaga tcccggtcac gattctcagt cgctgcctgc
     4021 agttcaacct gaagcagatg ccgcgcgaag ccatcacgga gcatctgcag ggggtcctcg
     4081 gcaaggaagg cattcaacac gagcccgaag cgctttccct cctcgcgcgc gccgcggcag
     4141 gcagcatgcg cgacgcactc tcgctcctcg accaggcgat cgcgcacggc ggcggcagcg
     4201 tcggcgcggc gagcgtcggc gacatgctcg gcacgatcga ccagggccac ctcgtgcggc
     4261 tgctcgaggc cgtcgccgcg ggcgacgcgg ccggcgcggt gcagatcgcc gacgagatgc
     4321 aggcgcggag cctttcgttc gacactgcgc tcgcggacct ggcgagcctc ctgctgcagg
     4381 tcgcgctggc ccagtcgctc gccgacgcgg tggacgacgc ccgcgcacgc gagctcgcga
     4441 cgcgcatcga cgcggagtcg gtccagctct actaccagat cgcgctgcag ggcagggaag
     4501 acctgccgct cgctcccgac gagcacgccg gtttcgtcat gaccgtgctg cgtctgctcg
     4561 cgttccggcc cgagggcgcg ccgaaaggag tggcgttacc caaggcggcg aagcccgcac
     4621 ctcagaattc aaagccaacc ggctggcccg agctggtgca gcagcttgcc gtcaccggcg
     4681 ctgcgcgcga gctcgcccgc aacgccgagc tcaagcagcg ccaggcctcg agcttcgagc
     4741 tggtggtgcc caaggcgaag gcgtacctcg ccgagcgcag ctacgtcgac aagctgaagg
     4801 ccgccctgga gcagcatctc gggggcgcgg taagcctgaa agtctcggcc ggcgaggtcg
     4861 cgggcggcag cgtcgcctcg ctcgaagcgg gcgagcgcga agcgcggcgc gccgccgccg
     4921 cgcaggcggt gaaaagcgac ggctttgtgc aggacctcgt caacatgttc gacggaaaga
     4981 tcgtcgattc gactatccgg ccggaaggga aataagacgc tcatgaaagg caacatcggg
     5041 cagctgatga agcaggcgca gcagatgcag gaaaacatgc gccgcatgca ggagagcctc
     5101 gccgtcacgg aggtcgaggg ccagtcgggc gccggcatgg tgaaggtcca gatgacctgc
     5161 aagtacgagg tgaagcgcgt gaccatcgac ccttcgctcg tcggcgacga ccgcgagatg
     5221 ctcgaggacc tggtggtggc cgcgttcaac gacgccgcgc gcaaggccga ggcgacgatc
     5281 gccgagaaga tgggcggcat gaccgcaggg ctcggcctgc cgccgggatt caagctgccg
     5341 ttttgagcaa gaccccggtc cccaccgcca aagggctcga gcggctggtg caggccttgc
     5401 gcgtgctgcc gggcgtcggg ccgcgctcgg cgcagcggat ggcgttccac cttctgcagc
     5461 acgagcgcgg cggcgccgag gagctcgcgc gcgcgcttgc ggaggcgctc gcgagcgtgc
     5521 agcgatgtgc gcagtgcaac aacttcagcg aggacgaggt gtgcgcgctg tgccgctcgc
     5581 cgcgccgcga ccgctcgctg ctgtgcgtgg tcgagacgcc ggccgacctg gcgatggtcg
     5641 agcagacgct ctcctattcg ggcatgtact tcgtgctgat ggggcgcctg tcgccgctcg
     5701 acggcgtcgg cccgcgcgat atcggcctgg accggctgct cgcgcgcgcg gccgagggcg
     5761 aggtgcagga ggtgatcctc gccacgaact tcaccaacga gggcgaggcg accgcgcact
     5821 acatcgccga gatgctgcgc gcgcgcagcg tcagggcgac gcgcatcgcg cgcggcgtgc
     5881 ccctcggcgg cgagctcgag tacgtcgacg ccggcacgat ctcgcaggca ttgctcgacc
     5941 ggcgcgggat ctagatgcgc cttgccgttt ttgcagcact tagcgttctg gccgctgcgg
     6001 catgggggca ggcctatccg tccaaaccgg tccgggtcgt cgtgcc
//
LOCUS       DATBSZ010000224        10371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_451151, whole genome shotgun sequence.
ACCESSION   DATBSZ010000224 DATBSZ010000000
VERSION     DATBSZ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10371
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451151"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2229
                     /gene="fdhF"
                     /locus_tag="VIG70_14005"
     CDS             <1..2229
                     /gene="fdhF"
                     /locus_tag="VIG70_14005"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEY8251711.1"
                     /translation="CVACGECVQACPTGALMPAREAGLETIDKTVDSVCPFCGVGCLL
                     TYHVNTKQNVIKFVNGKDGPSNHNRLCVKGRYGFDYAAHPQRLTKPLIRKPGFPKTAH
                     FKVDPANFGEVFREASWEEALDFAANGLLKLKKADPYSLAGFGSAKGSNEEAYLFQKL
                     VRTGFGTNNVDHCTRLCHASSVAALLEGIGSGAVSNQVNDVENAEVIFLIGANPTSNH
                     PVAATWLKNAAKRGAKLIYADPRASDLSRHAWRMLQFKADTDVALLNAMIHTIIDENL
                     VNESFVKDRTSGYEALKENAKNFSPEKMAPICGIPAETIRECARAFATSKGSMILWGM
                     GISQHIHGTDNARCLIALCLMTGQVGKPGSGLHPLRGQNNVQGASDSGLIPMMYPDYQ
                     RVNTDEANQRFEKFWGTQLDKKPGLTVVEIVNAAYDGSIRGMYIMGENPAMSDPDVEH
                     ARAGMAKLEHLVVQDIFLTETAYLADVVLPATAWPEKTGTVTNTDRMVQLGRKAVEPP
                     GEAREDFRILVQLGQRLGLDWKYEHPKQVFDEMRGCMDSIKGITWERLERDSSVTYPC
                     ENEGDPGQPVVFTTHFPTPTGRAKFVPADLIPAAERPDGEYPMVLITGRQLEHWHTGA
                     MTRRAGVLDAIEPEPVASLHPLEIEKLGIKAGDVVTVESRRGKISLYARADAGTPRGA
                     VFIPFAFYEAAANMLTNPVLDPFGKIPEFKYCAVKVTKGGARPGDPGYGKGRTLEEIY
                     GK"
     gene            2245..3069
                     /locus_tag="VIG70_14010"
     CDS             2245..3069
                     /locus_tag="VIG70_14010"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEY8251712.1"
                     /translation="MPVIAITREMGSLGKDVARILGAELKVPVVYHEVIDHLADRMRV
                     RKSHVIRLLDGRASLLERLTADKTSLSLFTADEIIGVAASGNGAVIRGWGATHLLREV
                     AHAVCVRVCAPFETRKRRMMERLSSDDEDQVGEEIRTNDEAHGAIMRRHFGVQWTDSE
                     HYDVVLNTKRVSVEECVNEVLALVRSPQFAETERSRSKLGDLALAASVRAALRRAAET
                     RDAQVAVSAEDGRVTLSGAGSTDEMLAFVEVASAVPGVLDVAYRTHPGEDLRPRFH"
     gene            3077..3493
                     /locus_tag="VIG70_14015"
     CDS             3077..3493
                     /locus_tag="VIG70_14015"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8251713.1"
                     /translation="MKILVAVDGSKPSLKVVQRLVEHCDWYRERPEVDLVTVHLPVPK
                     VGHIAKAQLDRYYAEEGEGNLAAAKKKLDAAGVPYRARILVGPIAESLVKHAKDQRCD
                     LIYIGTRGLSGVGAAILGSTAIKVLHISETPVLLVK"
     gene            complement(3652..4806)
                     /locus_tag="VIG70_14020"
     CDS             complement(3652..4806)
                     /locus_tag="VIG70_14020"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251714.1"
                     /translation="MKSPNFTLKATAAGLALAAAALGAQAQTAPGVSSTTVLFGQSTP
                     LSGANAELGNDIRNGALAYFKKVNDAGGVHGRKIELNSLDDANQVPRAEANTKKLVEE
                     DRVFALFGYASATLSRPALPIVAKHNVPFLAPFTGADPMRVFNKNVYNMRASYADELE
                     KIVEHYILLGSKRFSIVHYDDVVGKENLTAVERALKKHNMAPVSVAAFKDRAKPEIDA
                     GVKSVAQGTPDVVILTTLYKASSDFVKASRKSGLQAQMVSNSFPGASPLAKELGEHGA
                     GVAVAQVVPPPTQRSRPVVREYQEAITKLLGKKEFSFTSLEAFISAKITVEALKRAGA
                     RPTREGFMNALDNMKDVDMGGYFVNFSPTNHNGSNQVEMTVIGSQLKFNY"
     gene            complement(4841..5230)
                     /locus_tag="VIG70_14025"
     CDS             complement(4841..5230)
                     /locus_tag="VIG70_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251715.1"
                     /translation="MKKLNVMAMVVAAVMLPVALGPAAAADDRVLGEKLDSGLGTMVY
                     GESLDSGLGDLPATYTAAEYMPAGWVRGESLDSGLGELPATYTAAEYMPAGWVRGESL
                     DSGLGELASDYTAAEFMPKTKIVSAER"
     gene            5484..7154
                     /locus_tag="VIG70_14030"
     CDS             5484..7154
                     /locus_tag="VIG70_14030"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEY8251716.1"
                     /translation="MPSDIEIAQAAKMQRVAAMAREKLGIAEEHLEPYGHYKAKVSLK
                     YLDSLKGRKNGKLILVTAMTPTPPGEGKTTTTVGLGDALNHIGKKAVICLREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFGAIQLANNLLAAMLDNHIHHGNELGIDVR
                     RIQWKRVLDMNDRALRETVVSLGGPGNGYPRQEGFDIVVASEVMAIFCLATSLEDLKK
                     RLGSIVVGYTRDQRPVTAKDLNAHGAMTVLLKDALAPNLVQTLENNPAFIHGGPFANI
                     AHGCNSVIATQSALKLADYVVTEAGFGADLGAEKFLDIKCRKSGLRPNAAVIVATIRA
                     LKYHGGVDAKEVNKENLAALDKGLVNLERHVENVSKVYGIPCVVSINRFTADTKAELE
                     LLDSRMKKLGVPVVLATHWGDGGKGAAELARIAVGLCEQPSKPTFVYEDTDPLWEKIR
                     KIAQKVYRAGDVSADAKVRNQIKKLQDDGYGHYPVCVAKTQYSFSADASLRAAPSGHV
                     VNVREVRLAAGAEFVVMICGDIMTMPGLPKVPSANKIDLVDGKVVGLF"
     gene            7164..7925
                     /locus_tag="VIG70_14035"
     CDS             7164..7925
                     /locus_tag="VIG70_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY8251717.1"
                     /translation="MLEELASQAFWVGLLKIIGVNLILSGDNAVVIALAARSLPARQQ
                     KAAVLWGSGAAVVMRIILTIFAVALLTLPWLKLIGSLLLFWIGVKLLLPEEGGADVDA
                     SDNLIAAIKTILIADLVMSIDNVIAVAAAAQGSLTLLILGLAISIPLVIFGSTMLLHL
                     MERWPVIITIGGGLLGFVAGEMLVTDPALKGWLAGLGVEFDGEKPKVGGISLEILCGA
                     IGAVIVIAAGTFLGKRRAMKAAVHEAAVESERPKS"
     gene            8031..9032
                     /locus_tag="VIG70_14040"
     CDS             8031..9032
                     /locus_tag="VIG70_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY8251718.1"
                     /translation="MRQAFRSTVVLAAALATPFAAQAQSWEPTKNVEFIIPAGTGGGA
                     DQMARAVQGIVAKHNLMKQSILPINKAGGAGAEGFLDVKNAAGDPHKIIITLSNLFTT
                     PLATGVPFNWRDLTPVQMLALDNFVLWVNAKTPYKSAKEFIEAVKKAGPGKMKMGGTG
                     AKQEDQIITVAVEQKTGAKFTYIPFKGGGAVATQLVGGHVDATVNNPIEAVAQWRGGN
                     LRPLCVFELKRLPYKDKVTDKMAWSDIPTCKEAGLDVDYLMLRGIFMPPGVKPEHVRY
                     YTELFKKVMATPEWKKLMEEGAFNTTALAGAQYADWVAREEARHVQLMKAAGFIAGQ"
     gene            9034..9534
                     /locus_tag="VIG70_14045"
     CDS             9034..9534
                     /locus_tag="VIG70_14045"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY8251719.1"
                     /translation="MSADKPAFRRKSAELAVAALFFIFGAIVITDSVRLGFGWQEVHG
                     PRPGYFPFYIGLIICIASLVNIVHAVRVPAAQNKTFVEVGQLKLVLTVLVPTAIYAAL
                     VTWIGIYAASVLFIGFFMRWLGKYSWWKVVATSVTTVVVFYVIFEKWFKVPLPKGPLE
                     SILGLN"
     gene            9551..>10371
                     /locus_tag="VIG70_14050"
     CDS             9551..>10371
                     /locus_tag="VIG70_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY8251720.1"
                     /translation="MEELTALAHGFGVALTPTNIALMFTGVILGVIIGVLPGLGGANG
                     VAILLPLTFGMAQTPGGATSAIILLSCIYWGALFGGAITSILFNIPGEPWSVATTFDG
                     YPLAQQGRAGTALTAAFTSSFVGAFVAVVVITFLSPVVAGFALEFGPPEFFAVYLLTF
                     CAFVGTSKEPAFKTIAAMMLGFALAAVGLDTVTGTLRLTFGSTHLLTGFDFLVAVIGL
                     FGIGEILLTLEEGLAFRGANARINVRVVLDTWKELPRYWLTSLRSSAVGCFMGIVP"
BASE COUNT         1806 a         3383 c         3478 g         1704 t
ORIGIN      
        1 tgcgtcgcct gcggcgagtg cgtgcaggcc tgcccgaccg gcgcgctgat gccggcgcgc
       61 gaagccggcc tcgagaccat cgacaagacg gtggactcgg tgtgcccgtt ctgcggcgtc
      121 gggtgcctgc tcacctacca cgtcaacacg aagcagaacg tcatcaagtt cgtcaacggc
      181 aaggacgggc cgtccaacca caaccgcctc tgcgtcaagg gccgctacgg cttcgactac
      241 gccgcgcacc cgcagcgcct caccaagccc ctcatccgca agccgggttt cccaaagacg
      301 gcgcacttca aggtcgatcc agccaacttc ggggaagtgt tccgcgaggc gagctgggaa
      361 gaggctctcg atttcgcggc caacggtctc ctgaagctga aaaaagcaga tccctattcc
      421 ctggccggct tcggttcggc caagggttcg aacgaggagg cgtacctctt ccagaagctg
      481 gtgcgcaccg gcttcggcac caacaacgtc gaccactgca cgcggctgtg ccacgcctcg
      541 tcggtggcgg cgctgctcga gggcatcggc tcgggcgcgg tgtcgaacca ggtgaacgac
      601 gtcgagaacg ccgaggtgat cttcctgatc ggcgccaacc cgacctcgaa ccacccggtg
      661 gcggcgacct ggctgaagaa cgccgcgaag cgcggcgcga agctgatcta cgccgacccg
      721 cgggcgagcg acctctcgcg ccatgcgtgg cgcatgctgc agttcaaggc cgacaccgac
      781 gtcgcgctgc tcaacgcaat gatccacacg atcatcgacg aaaaccttgt caatgagtct
      841 tttgtaaaag accgaaccag cggctacgag gctctgaagg aaaacgcgaa gaacttctcg
      901 ccggagaaga tggcgccgat ctgcggcatc ccggccgaga ccatccgcga atgcgcgcgc
      961 gcgttcgcca cctccaaggg ctcgatgatc ctgtggggaa tggggatctc gcagcacatc
     1021 cacggcaccg acaacgcgcg ctgcctgatc gcgctgtgcc tgatgaccgg ccaggtcggc
     1081 aagcccggct ccgggctgca tccgctgcgc ggccagaaca acgtgcaggg cgcttccgat
     1141 tccggcctga tcccgatgat gtacccggac taccagcgcg tgaacaccga cgaggcgaac
     1201 cagcgtttcg agaagttctg gggcacgcag ctcgacaaga agcccgggct gaccgtggtc
     1261 gagatcgtga acgcggccta tgacggaagc atccgcggca tgtacatcat gggcgagaac
     1321 cccgcgatgt cggacccgga cgtcgagcac gcgcgcgccg gcatggcgaa actggagcac
     1381 ctcgtcgttc aagatatttt cttgacggaa acggcgtatc tggctgacgt ggtgctgccc
     1441 gccaccgcgt ggccggagaa gaccggcacg gtcaccaaca ccgaccgcat ggtgcagctc
     1501 ggtcgcaagg cggtcgagcc gccgggcgag gcgcgcgagg acttccggat cctggtgcaa
     1561 ttgggccagc gcctgggcct ggattggaaa tacgaacacc ccaagcaggt gttcgacgag
     1621 atgcgcggct gcatggactc gatcaagggc atcacctggg agcgcctgga gcgcgactcg
     1681 tcggtgacct atccctgcga gaacgagggc gacccgggcc agccggtggt gttcaccacg
     1741 cacttcccga cgcccacggg gcgcgcgaag ttcgtgcccg ccgacctgat ccccgccgcg
     1801 gagcgcccgg atggcgagta tccgatggtg ctgatcaccg gccgccagct cgagcactgg
     1861 cacaccggcg cgatgacgcg ccgcgccggc gtgctcgacg cgatcgagcc cgagccggtg
     1921 gcatcgctgc atccgctgga aatcgagaaa ctcgggatca aggcgggaga cgtggtcacg
     1981 gtcgaatcgc gccgcggcaa gatttccctc tatgcaaggg ccgatgcggg cacgccgcgc
     2041 ggggcggtgt tcatcccctt cgcgttctac gaggcggcgg ccaacatgct caccaacccg
     2101 gtgctcgacc cgttcggcaa gatcccggag ttcaagtact gcgcggtgaa agtgaccaag
     2161 ggcggcgcaa ggccggggga cccgggctac ggcaaggggc gtacactgga agagatctac
     2221 ggcaagtagg agcgaggggg agctatgccg gttatcgcga tcacgcggga aatgggttcg
     2281 ctcggcaagg acgtggcgcg catcctcggc gccgagctca aggtgccggt ggtgtaccac
     2341 gaggtcatcg accatctggc ggaccgcatg cgcgtgcgca agagccacgt cattcgcctg
     2401 ctcgacggcc gcgccagcct gctcgagcgc ctcaccgccg acaagaccag cctttcgctc
     2461 tttaccgccg acgagatcat cggcgtggct gcgagcggca acggcgccgt gatccgcggc
     2521 tggggcgcga cccacctgct gcgcgaggtg gcgcacgcgg tgtgcgtgcg ggtgtgcgcg
     2581 ccgttcgaga cgcgcaagcg ccgcatgatg gagcgcctgt cgagcgacga cgaggaccag
     2641 gtcggcgagg agatccgcac gaacgacgag gcgcacggcg cgatcatgcg gcgccatttc
     2701 ggcgtgcagt ggaccgactc ggagcactac gatgtcgtcc tcaacaccaa gcgcgtgagc
     2761 gtcgaggagt gcgtgaacga ggtgctggcg ctggtgcgtt cgccgcagtt cgccgagacc
     2821 gagcgctcgc gctcgaagct cggcgacctg gcgctggccg ccagcgtgcg cgcggcgctg
     2881 cgccgcgccg ccgagacgcg cgacgcgcag gtcgcggtga gcgccgagga cggccgcgtc
     2941 acgctctcgg gcgccggcag caccgacgag atgctcgctt tcgtcgaggt cgcctccgcg
     3001 gtgcccggcg tgctcgacgt cgcgtaccgc acccacccgg gcgaggacct gcggccccgc
     3061 ttccactgag ggattcatga aaattctggt tgccgtcgac ggctccaagc cgtcgctgaa
     3121 ggttgtgcag cggctcgtcg agcactgcga ctggtaccgc gagcgtcccg aggtggatct
     3181 cgtcacggtg cacctgccgg tgcccaaggt ggggcacata gccaaggcgc aactcgacag
     3241 gtattacgca gaggaaggcg aggggaacct cgcggcggcg aagaagaagc tcgatgcggc
     3301 cggcgttccc taccgggcgc gcatcctggt cggcccgatc gccgagtcgc tggtcaagca
     3361 tgcgaaggac cagcgctgcg acctcatcta catcggcacg cgcggcctga gcggggtcgg
     3421 cgcggcgatc ctgggttcca ccgccatcaa ggtcctgcac atctccgaga ccccggtgct
     3481 actggtcaag tagcggcgcc ggcaaaaaaa agggggaagc ggccggtaaa tccccccatc
     3541 cccgtttggc cgcttccccg caaaactgtg cttgtccgat ccctcagcta agaaaaaggg
     3601 gggagcggcc agaaatcccc ttccccattg gccgctcccc cggaggaacc gctagtagtt
     3661 gaacttcagc tggctgccga tcacggtcat ctcgacctga ttgctgccgt tgtggttggt
     3721 gggcgagaag ttcacgaagt agccgcccat gtcgacgtcc ttcatgttgt cgagcgcgtt
     3781 catgaagccc tcgcgcgtcg ggcgcgctcc ggcgcgcttc agcgcctcga ccgtgatctt
     3841 ggccgagatg aacgcctcga gcgacgtgaa ggagaactcc ttcttgccga gcagcttggt
     3901 gatggcttcc tggtattcgc gcaccaccgg ccgcgagcgc tgggtcggcg gcggcactac
     3961 ctgggcgacc gcgaccccgg cgccgtgctc accgagctcc ttggcgagcg ggctcgcgcc
     4021 cgggaaggag ttgctcacca tctgggcctg cagcccggac ttgcgcgatg ccttgacgaa
     4081 gtcggagctc gccttgtaca gcgtggtgag gatcacgacg tcgggcgtgc cctgcgcgac
     4141 gctcttcacg ccggcgtcga tctccggctt ggcgcggtcc ttgaaggccg ccaccgacac
     4201 cggcgccatg ttgtgcttct tcagcgcgcg ctccaccgcg gtcaggttct ctttaccgac
     4261 cacgtcgtcg taatgcacga tcgagaagcg cttgctgccg agcaggatgt agtgctcgac
     4321 gatcttttcc agctcgtcgg cgtagctcgc gcgcatgttg tacacgttct tgttgaacac
     4381 gcgcatcggg tcggcgccgg tgaagggcgc gaggaacggc acgttgtgct tcgccacgat
     4441 cggcagcgcc ggccgcgaca gggtcgcgct cgcgtagccg aacagggcga acacgcggtc
     4501 ttcctcgacc agcttcttgg tgttcgcttc cgcgcgcggc acctggttcg catcgtcgag
     4561 cgagttgagc tcgatcttgc ggccgtgcac gccgccggcg tcgttcacct tcttgaagta
     4621 ggcgagcgcg ccgttgcgga tgtcgttacc gagctcggcg ttggcgcccg acagcggcgt
     4681 cgactggccg aaaagcacgg tggtggagct gacgcccggg gcggtctgcg cctgggcgcc
     4741 gagggctgcg gctgcgagcg cgaggcccgc cgccgtggcc ttgagggtga agttcgggga
     4801 tttcatgggg gaatctccgt tgttaggcgc cggcgttcgg ctagcgctct gctgaaacga
     4861 tcttggtctt cggcatgaac tcggccgcgg tgtagtccga ggccagctcg ccaaggccgc
     4921 tgtcgaggct ttcgccgcgc acccagccgg ccggcatgta ctccgccgcc gtataagtgg
     4981 ccggcagctc gccaaggccg ctgtcgaggc tttcgccgcg cacccagccg gccggcatgt
     5041 actcggccgc cgtataagtg gccggcaggt cgcccaggcc gctgtctagg ctttcgccgt
     5101 acaccatcgt gcccaggccg ctgtcgagct tttcgcccag gacccggtca tccgccgcag
     5161 cggccggacc gagcgctacc ggcagcatca ccgctgccac caccatcgcc atcacgttga
     5221 gcttcttcac aatttcctcc gttaattcaa gttggggtcg gactgcaggc gcaagatgcc
     5281 cgagcgggcg gcgcgcttcc gtgatccagt gctcgacttg acagaccctg atggacgcgt
     5341 gacagaccct gataagggca tgacagtcgc tgatgggatc tgacagtccc tgatggggca
     5401 tgacagtccc tgtagattga ccgccatcaa ggccgcggcg cggcccctct tacagaatcg
     5461 ccgcttccac gaaaaggaac gtcatgccgt ccgatatcga gatcgcccag gcagcaaaga
     5521 tgcagcgcgt cgccgcgatg gcgcgcgaga agctcgggat cgccgaggag cacctcgagc
     5581 cctacggcca ctacaaggcc aaggtctcgc tcaagtacct cgactcgctc aagggcagga
     5641 agaacggcaa gctgattctc gtcacggcga tgacgccgac gccgccgggc gaaggcaaga
     5701 ccacgaccac cgtgggactg ggcgacgcgc tcaaccacat cggcaagaag gcggtgatct
     5761 gcctgcgcga gccctcgctc gggccggtgt tcggcgtaaa aggcggggcc gccggcggcg
     5821 gctacgcgca ggtcgtgccg atggaggaca tcaacctgca cttcaccggc gatttcggtg
     5881 cgatccagct cgcgaacaac ctgctcgcgg cgatgctcga caaccacatc caccacggca
     5941 acgagctcgg catcgacgtg cgccgcatcc agtggaagcg cgtgctcgac atgaacgacc
     6001 gcgcgctgcg cgagaccgtg gtgtcgctcg gcgggccggg caacggctat ccgcgccagg
     6061 agggcttcga catcgtcgtc gcctccgagg tgatggcgat cttctgcctc gcaacgtcgc
     6121 tcgaggatct caagaagcgt ttgggaagca tcgtggtcgg ttacacgcgc gatcaacggc
     6181 cggtcactgc caaagatcta aatgcccacg gcgcgatgac cgttctgctc aaggacgcgc
     6241 tcgcgccgaa cctggtgcag acgctcgaga acaaccccgc gttcatccac ggcggcccgt
     6301 tcgccaacat cgcgcacggc tgcaactcgg tcatcgcgac gcagagcgcg ctcaagctcg
     6361 ccgactacgt ggtcaccgag gcgggcttcg gcgccgacct cggcgccgag aagttcctcg
     6421 acatcaagtg ccgcaagtcc gggctgcggc cgaacgccgc ggtcatcgtg gcgacgatcc
     6481 gcgcgctcaa gtaccacggt ggcgtcgacg cgaaggaagt gaacaaggag aacctcgccg
     6541 cgctggacaa gggattggtg aacctggagc gccatgtcga gaacgtgtcg aaggtgtacg
     6601 ggattccctg cgtggtgtcg atcaaccgct tcaccgcgga taccaaagct gaactggaat
     6661 tactggattc cagaatgaag aaactcggcg tcccggtggt cctggcgacg cactggggcg
     6721 acggcggcaa gggggcggcc gagctggccc ggattgcggt cgggttgtgc gagcagccct
     6781 cgaagccgac cttcgtctac gaggacaccg acccgctgtg ggagaagatc cgcaagatcg
     6841 cgcagaaggt gtaccgcgcc ggcgacgttt ccgccgacgc caaggtgcgc aaccagatca
     6901 agaagctgca ggacgacggc tacggtcact acccggtgtg cgtggcgaag acgcagtact
     6961 cgttctccgc cgacgcaagc ctgcgtgccg cgccgagcgg ccacgtggtg aacgtgcgcg
     7021 aggtgcggct cgccgccggc gcggagttcg tggtgatgat ctgcggcgac atcatgacca
     7081 tgccgggcct gccgaaggtt ccgtcggcca acaagatcga tctggtggac ggcaaagtcg
     7141 tggggctgtt ctagaataag acgatgctgg aggagctcgc gtcgcaggcc ttctgggtcg
     7201 gcctgctgaa gatcatcggc gtcaacctca tcctgtcggg cgacaacgcg gtggtgatcg
     7261 cgctcgccgc gcgctcgctg ccggccaggc agcagaaggc cgccgtgctg tggggctcgg
     7321 gcgcggcggt ggtgatgcgg atcatcctca cgatcttcgc cgtcgccctg ctcacgctgc
     7381 cctggctgaa gctgatcggc agcctgttgc tgttctggat cggcgtcaag ctcctgctcc
     7441 ccgaggaggg gggcgcagac gtcgacgcaa gcgacaacct gatcgcggcg atcaagacca
     7501 tcctcattgc cgatctggtg atgagcatcg acaacgtcat cgcggtggcc gcggcggcgc
     7561 agggcagcct gaccctgctg atcctgggcc tcgcgatctc gatcccgctg gtgatcttcg
     7621 gctcgaccat gctcctccat ttgatggagc gctggccggt catcatcacc atcggcggcg
     7681 gcctgctcgg cttcgtcgcc ggggaaatgc tggtcaccga cccggcgctg aagggctggc
     7741 tggcgggcct gggcgtcgaa ttcgacggcg agaagcccaa agtgggcggc atcagcctgg
     7801 aaatcctctg cggggcgatc ggcgcggtca tcgtgatcgc cgctggaacg tttctcggca
     7861 agcgcagggc catgaaggcg gccgttcacg aagcagcagt ggagagcgaa cggccgaagt
     7921 cttgatcgtg gtcaaggctg cgcggcaacg gggagcatga gaattctgcg ataccaagca
     7981 ccacgagcga atggtgcgtc gcagcgaagg gtgaacctat cggaggagtc atgagacaag
     8041 cgttccgcag caccgtggta cttgccgcag cactcgccac gccgttcgcg gcgcaggccc
     8101 agtcctggga gccgaccaaa aacgtcgagt tcatcatccc ggcgggcacc gggggcggcg
     8161 ccgaccagat ggcgcgcgca gtccagggca tcgtcgccaa gcacaacctg atgaagcagt
     8221 cgatcctgcc gatcaacaag gcgggcggcg ccggcgccga gggcttcctc gacgtgaaga
     8281 acgctgccgg cgatccgcac aagatcatca tcacgctgtc gaacctgttc accacgccgc
     8341 tcgccacggg cgtgccgttc aactggcgcg acctcacgcc ggtgcagatg ctggcgctcg
     8401 acaacttcgt gctctgggtg aacgccaaga cgccctacaa gagcgccaag gagttcatcg
     8461 aggcggtgaa aaaggccggc ccgggcaaga tgaagatggg cggcaccggc gccaagcagg
     8521 aagaccagat catcaccgtg gcggtcgagc agaagaccgg ggcgaagttc acctacatcc
     8581 cgttcaaggg cggcggcgcg gtggcgacgc agctggtcgg cggccacgtc gacgcgacgg
     8641 tgaacaaccc gatcgaggcg gtggcgcaat ggcgcggcgg caacctgcgg ccgctgtgcg
     8701 tgttcgagct caagcgcctg ccgtacaagg acaaggtcac cgacaagatg gcgtggagcg
     8761 acatcccgac ctgcaaggag gccgggctcg acgtcgacta cctgatgctg cgcggcatct
     8821 tcatgccgcc gggcgtgaag cccgagcacg tgcgctacta cacggagctc ttcaagaagg
     8881 tgatggcgac gcccgagtgg aagaagctga tggaggaggg ggcgttcaac accaccgcgc
     8941 tcgccggcgc gcagtacgct gactgggtcg ctcgcgagga agcgcgccat gtccagctca
     9001 tgaaagctgc cggctttatc gcggggcaat agcataagcg cagacaaacc tgcgtttcgg
     9061 cggaagagcg cggagcttgc ggtagccgcg ctcttcttca ttttcggcgc catcgtcatc
     9121 accgacagcg tgcgcctggg cttcggctgg caggaggtgc acgggccgcg gccgggctac
     9181 ttcccgttct acatcggcct gatcatctgc atcgcgtcgc tcgtcaacat cgtgcacgcg
     9241 gtgcgcgtcc cggccgcgca gaacaagacg ttcgtcgagg tcgggcagct caagctggtg
     9301 ctcacggtgc tcgtcccgac ggcgatctac gcggcgctgg tgacctggat cgggatctac
     9361 gccgcttcgg tcctgttcat cggcttcttc atgcgctggc tcgggaagta ttcctggtgg
     9421 aaggtggtcg ccacgagcgt caccaccgtg gtcgtgttct acgtgatctt cgagaagtgg
     9481 ttcaaggtgc cgctgcccaa ggggccgctg gaatcgatcc tgggattgaa ctagttgaaa
     9541 gaagaatgaa gtggaagagc tgacggccct cgctcacggc ttcggcgtgg cgctcacgcc
     9601 gaccaacatc gcgctgatgt tcaccggcgt gatcctgggc gtgatcatcg gcgtgctgcc
     9661 ggggttgggc ggcgcgaacg gcgtcgcgat cctgctgccg ctcaccttcg gcatggcgca
     9721 gactcccggc ggcgccacct cggcgatcat cctgctgtcg tgcatctact ggggcgccct
     9781 cttcggcggc gcgatcacct cgatcctgtt caacatcccg ggcgagccgt ggtcggtggc
     9841 cacgaccttc gacggctatc cgctggcgca gcagggccgc gccggcacgg cgctgactgc
     9901 cgccttcacc tcctcgttcg tcggcgcgtt cgtcgccgtg gtggtgatca ccttcctctc
     9961 gccggtggtc gccggcttcg cgctcgagtt cggcccgccc gaattcttcg cggtgtacct
    10021 gctcaccttc tgcgccttcg tcggcaccag caaggagccg gcgttcaaga ccatcgctgc
    10081 gatgatgctc ggcttcgccc tcgccgcggt cggcctcgac acggtcaccg gcacgctgcg
    10141 cctgaccttc ggctcgacgc acctgctcac cggcttcgat ttcctcgtgg cggtgatcgg
    10201 cctcttcggc atcggcgaga tcctgctcac gctcgaggag gggctcgcgt tccgcggggc
    10261 caacgccagg atcaacgtgc gcgtagtgct ggacacctgg aaggagctgc cgcgctactg
    10321 gctcacatcg ctgcgctcct cggcggtggg ctgcttcatg ggcatcgtgc c
//
LOCUS       DATBSZ010000225         3833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_382240, whole genome shotgun sequence.
ACCESSION   DATBSZ010000225 DATBSZ010000000
VERSION     DATBSZ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382240"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VIG70_14055"
     CDS             complement(<1..302)
                     /locus_tag="VIG70_14055"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8251721.1"
                     /translation="MKVSVVVPAFNEERLLAGSLTAIRAAARAFDDAGWACELIVCDN
                     NSTDRTAEIAREAGARVVFEPVNQIARARNAGAARASGEWLVFVDADSYPSRELFA"
     gene            complement(299..886)
                     /locus_tag="VIG70_14060"
     CDS             complement(299..886)
                     /locus_tag="VIG70_14060"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8251722.1"
                     /translation="MPLGVAVGHGARRSPAASKTIDVKVRRYRPSDRGAVIGLFRESM
                     WDLAPPTLAADFQSYIERAIQEELGKVEEYYFGREGQGFWVTQEGERPDRVLGMVGLE
                     RHADDAGELRRMAVEKAHRRKGIGRELLATAEAFCRDCGYRRVVLSTSELQVAAMRLY
                     ESSGYRRVREEAAAPASHKSVGAGLTRYHYEKPLA"
     gene            complement(817..1221)
                     /locus_tag="VIG70_14065"
     CDS             complement(817..1221)
                     /locus_tag="VIG70_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8251723.1"
                     /translation="MRIDGACHCGRITYEAEVDPEKAGLCHCTDCQTLSGSAFVAFVP
                     VPKEAFRLRGQPKIYVKTAESGSRRAQAFCPDCGTRLYASAEKDPQVFNLRIGTVRQR
                     AALPPKSQVWCRSALPWVMELAEVQQHPKQSM"
     gene            complement(1237..1794)
                     /locus_tag="VIG70_14070"
     CDS             complement(1237..1794)
                     /locus_tag="VIG70_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251724.1"
                     /translation="MATLNPQQSTQQKVAQYLLLGVLEDALNLVQRYQQGGGFKNYVL
                     ERMRLIVPVGLLMGVTSIGCAAATVLYLGGTRPTLVLLSILLVPFVLLGSFFVQAYVF
                     AFWLENRALAQALHHKPAAAGTVAARLRKAGIKVGALPPVPWALAALVLFLPLGMLII
                     VIPWLGILLTALLIIAPVAFARLDR"
     gene            complement(1868..2173)
                     /locus_tag="VIG70_14075"
     CDS             complement(1868..2173)
                     /locus_tag="VIG70_14075"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEY8251725.1"
                     /translation="MRLAEVLPELVSDLETGLAHLGRGDLVQQLKDATIARWTYDDFS
                     DTTYLQLSATPVAMMNVERLSLYDELGVNLDSDSDGRLCGIEVLEGSRIASRLRNDA"
     gene            complement(2170..2766)
                     /locus_tag="VIG70_14080"
     CDS             complement(2170..2766)
                     /locus_tag="VIG70_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY8251726.1"
                     /translation="MRIAVLTFEGFNELDSFIAAAMVNRVKQPELRALIVCPAERVAS
                     MNGVVVHTQAPLSFAREADAVIVGSGAGTRELVRNPQIMGELRLDPARQVIAAQCSGA
                     LVLAKLGMLDDVPACTDSKTKPWVEEAGVKVLDQPFVAHRNVATAGGCLASHYLAAWV
                     IARLAGRKAADSVLHYVAPVGEKPQYVERARAAIGPYL"
     gene            complement(2768..3649)
                     /locus_tag="VIG70_14085"
     CDS             complement(2768..3649)
                     /locus_tag="VIG70_14085"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8251727.1"
                     /translation="MTSAFDIADLRTSRNLQSPMRSGREASLEKLAVACLMCLVASTA
                     RSQSEYQDVFYSSGALRIQAYLYRPNGEGPFPAVIYNHGTRDGRERASVPFEHIGRML
                     TRAGYAVMVPERRGYGKSDGEIWWREVSNDQARVINRLQAETDDVLAGADYLRSLPHV
                     DPKRLALMGWSFGGVVTMLATARSSAFLAAVNQAGGALTWDGNPHMRSALTEAAEKST
                     TPTLFMVARNDRTTSSVTTLAGIFKKRNTPHALVIYEPFSPPHPTKGAPGHALFSVQG
                     AGLWEKDVIEFLGHQLR"
BASE COUNT          644 a         1279 c         1261 g          649 t
ORIGIN      
        1 tcggcgaaca gctcgcggct gggatacgag tcggcgtcga cgaataccag ccactcgccg
       61 ctcgcccgtg ctgcgccggc attgcgcgcg cgcgcgatct ggttcaccgg ctcgaacact
      121 acgcgcgcgc ccgcctcgcg cgcgatctcg gcggtgcggt cggtcgagtt gttgtcgcac
      181 acgatgagct cgcaggccca gccggcatcg tcgaacgctc gcgccgcggc gcggatcgcg
      241 gtgagcgatc cggcgagcag gcgttcctcg ttgaatgcgg ggacgacgac ggagaccttc
      301 acgccagcgg cttctcgtag tgatagcgcg tgagcccggc gcccaccgat ttatgcgatg
      361 cgggcgcagc cgcctcctcg cgaacgcggc gatagccgct cgactcgtag agccgcatcg
      421 ccgcgacctg gagctcggaa gtgctgagca ccacccggcg gtacccgcaa tcccggcaga
      481 acgcctcggc ggtggcgagc agctcgcgcc cgattccctt gcggcggtgc gctttttcga
      541 ccgccatgcg ccggagctcg cccgcgtcat cggcgtgtcg ctcgagcccc accatgccca
      601 agacgcgatc gggcctttct ccttcctggg taacccagaa gccctgtccc tcgcggccga
      661 agtagtactc ctcgaccttg ccgagctctt cctggatggc gcgctcgatg taggactgaa
      721 agtccgcggc cagggtcggc ggggcgaggt cccacatgga ctcgcggaac agcccgatca
      781 cggcgcctcg gtccgaaggc cggtagcgcc gtaccttcac atcgattgtt ttggatgctg
      841 ctggacttcg gcgagctcca tgacccacgg caacgccgag cggcaccaga cctggctctt
      901 cggcgggagc gcggcgcgct ggcgcacggt gccgatgcgc aggttgaaca cctgggggtc
      961 tttctccgcc gacgcataca ggcgtgtgcc gcagtccggg cagaaggcct gcgcgcgccg
     1021 gctgccgctc tccgcggtct ttacgtagat cttcggctgg ccgcgtagcc ggaacgcttc
     1081 cttcggcacg ggcacgaagg cgacaaacgc cgaccccgac agggtctggc agtcagtgca
     1141 gtggcagagg ccggcctttt cagggtcgac ttccgcctcg taggtgatgc ggccgcagtg
     1201 gcacgcgccg tcgattcgca tgccctgcaa tgtacctcag cgatcgaggc gggcgaaggc
     1261 gaccggcgcg atgatcagca aggcagtcag gagaatgccg agccagggga tgacaatgat
     1321 cagcatccct agcggaagaa acaggaccag cgccgccaac gcccacggga ccggcggcag
     1381 ggctcccact ttgatcccgg ccttgcgcag ccgggccgcg accgtccccg ccgccgccgg
     1441 cttgtggtgc agagcctgcg ccagcgcgcg gttctcgagc cagaaggcaa aaacgtaagc
     1501 ctggacgaag aagctgccga gcagcacgaa gggaacgagc aggatcgaaa gcaggacgag
     1561 cgtcgggcgc gttcctccaa gatacagcac cgtcgccgcg gcgcagccga tgctcgtgac
     1621 tcccatgagc aagccgaccg ggacaatgag cctcatgcgc tcgagcacgt agttcttgaa
     1681 cccccctccc tgctggtacc tttgcacgag attcagtgcg tcctccagca ccccgaggag
     1741 caggtattgc gccaccttct gctgggtgga ttgttgcggg ttgagcgttg ccatgacggc
     1801 ctcccttgat acacggtggg ccccagcata caaagccgcc gccggacgta cagaggcata
     1861 gttccgctta tgcgtcattc cgcagccggg aggcgatccg gcttccctcg aggacctcga
     1921 tgccgcacag gcggccgtcg ctgtcgctgt ccaggttgac gccgagctcg tcgtacagcg
     1981 aaaggcgctc gacgttcatc atggcgaccg gtgtcgcgct cagctgcaga taggtggtgt
     2041 ccgagaaatc gtcgtacgtc cagcgcgcga tggtcgcgtc tttcagctgc tgcacgaggt
     2101 cgccgcgtcc gaggtgggcg agcccggtct ccaggtcgga gacgagctcg ggcaggactt
     2161 ccgccagcct caaagatagg gtccgatcgc ggcgcgggcg cgctcgacgt attgcggctt
     2221 ttcgcccacc ggcgccacgt agtgcagcac gctgtccgca gccttcctgc ccgcgagccg
     2281 ggcgatgacc caggctgcaa gatagtgcga ggccaggcat ccgcccgccg tggccacgtt
     2341 gcggtgcgcg acgaacggct ggtcgagaac ttttacgccc gcttcctcca cccaaggctt
     2401 ggtcttcgag tcggtgcagg caggcacatc gtcaagcatg ccgagcttcg ccaggacgag
     2461 tgcgccggag cattgcgccg cgatgacctg ccgcgccggg tcgagccgca gctcgcccat
     2521 gatctgcggg ttgcgcacca gctcccgcgt gccggcgccg ctgccgacga tcaccgcgtc
     2581 ggcctcgcgc gcgaagctca gcggcgcctg cgtgtggacc acgacgccat tcatcgacgc
     2641 gacccgctcg gcggggcaca cgatcagcgc gcgaagctcg ggctgcttga cccggttcac
     2701 catcgccgcc gcgatgaacg agtcgagctc gttgaatcct tcgaacgtca ggaccgctat
     2761 tcgcacgtca cctcagctgg tgtccaagaa attcgatcac gtctttttcc caaaggcccg
     2821 cgccctgcac ggagaacagg gcgtgtccgg gcgcgccctt ggtcggatga ggagggctga
     2881 agggctcgta gatcaccagg gcatggggcg tattgcgctt cttgaatatg cccgccagcg
     2941 tggtcacgga cgaagtagtg cggtcgttcc tggcgaccat gaacaaggtc ggggtcgtgg
     3001 atttctcggc agcctcggtg agagcgctcc gcatgtgcgg gttcccgtcc caggtcaggg
     3061 cgccgccagc ctgattcacg gctgcgagaa atgcagagct gcgggcagtg gccagcatcg
     3121 tgaccacgcc gccgaacgac caacccatca gcgcaagacg cttcgggtcg acatgcggca
     3181 ggctgcgcag atagtccgcg cctgccagca cgtcatccgt ttcggcctgc agccggttga
     3241 tcaccctcgc ctggtcgttg ctcacctcgc gccaccagat ttcgccgtcg gatttgccgt
     3301 agccgcgacg ctcggggacc atgacggcgt agccggctcg agtcaacatc cttccaatgt
     3361 gctcgaacgg aactgacgcg cgctcgcgac cgtctcgcgt gccgtggttg tagatcaccg
     3421 cgggaaatgg accttcgccg tttggcctgt acaaataggc ctggatccga agcgctccgc
     3481 tggaatagaa gacgtcctgg tactccgact ggcttcgggc ggtcgatgcc acgaggcaca
     3541 tcaggcacgc caccgcaagc ttttcaaggc tagcttcgcg tcctgatctc ataggtgact
     3601 gcaaattccg gctcgtacga agatcggcga tatcgaacgc agaagtcata gccgcttctc
     3661 tcggtgcaga cgtagttgtc cgtcagaata ctgccggtta tcttagcgcg gcggtgtcta
     3721 ggcggcgcgg cgcgagcgca ggaagggctc gacttcttcg gcggcgcgcg cgggggcgaa
     3781 cagctcgccc tggcagatgc ggcagccggc ctcgatgagg aagtcgcgct gcg
//
LOCUS       DATBSZ010000226         7649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_724944, whole genome shotgun sequence.
ACCESSION   DATBSZ010000226 DATBSZ010000000
VERSION     DATBSZ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724944"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="VIG70_14090"
     CDS             complement(<1..1253)
                     /locus_tag="VIG70_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEY8251728.1"
                     /translation="MKIAESSPVYASRRRRLAEAIGAGVAVVPTAPERVRNRDSHYPY
                     RFDSHFYYLTGFTEPEAVLVLASNKSILFCRERNPEREIWDGFRYGPEAARERFGFDE
                     AHPIANLDQEMARLLENQPALCYPMGADPEWDARAIRWLNAVRAKVRAGIAAPERVQD
                     VRAIVDDMRLVKDAHELGVMRRAARIAAQAHRRAMQRTRPGRTEYEIEAELLYEFRRS
                     GAQTPAYTPIVAGGANACVLHYVFNDAPLRDGDLLLIDAGCELDGYASDLTRTYPVNG
                     CFSAAQREVYEVVLAAQLAALDSVRPGSAWNEPHDAAVKVLAQGLIDLKLVPGSLDEV
                     LEKETYKRFYMHRTGHWLGLDVHDAGDYKRQGSWRALAPGMTLTVEPGLYIRAEDDIP
                     ERLRNIGIRIEDDVLVSDGGCEVLTA"
     gene            complement(1240..1914)
                     /locus_tag="VIG70_14095"
     CDS             complement(1240..1914)
                     /locus_tag="VIG70_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY8251729.1"
                     /translation="MKVMLLAAGRGERLRPLTDAIPKALVEAGGKPLIAWHLERLARA
                     GLAEAVINVSHLGERIVERIGDGARFGLRISYSRERERLETAGGIANALGLLGREPFL
                     LVNADVYCECDFSKLMKVKLDGRLAHLVLVPNPPHRPKGDFSLRDGRVGDEAAPRYTY
                     AGVAVMSPALVRPVTPGEKAPLAPLLYDAAARGLLGGELYEGLWQDVGTIERLAELEK
                     SLRENR"
     gene            complement(1911..2900)
                     /locus_tag="VIG70_14100"
     CDS             complement(1911..2900)
                     /locus_tag="VIG70_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8251730.1"
                     /translation="MIHADVRRDALERWLAPHLHGVSFTLSAASEDASFRRYWRATLE
                     DGASYVAMDAPPDKEDCRPFVRVARMLAEAGVHAPQVLAQDLERGFLLLSDLGTRTYL
                     AELNIDSGRNEAPRLFADATDALLRWQLATRPGALPPYDEALLRREMGLFPDWYVGKH
                     LKKSLSAEQQRTLEKIFALLVQSALAQPVVYVHRDYMPRNLMVCEPNPGVLDFQDAVL
                     GPITYDMVSLTRDAFLSWEEERVLDWSARYWEKAKRAGLPVAADFAEFWRAFEWMGLQ
                     RHLKVLGIFARINYRDGKPKYLEDTPRFLGYAREVSRRYAALAPLARLLEELA"
     gene            2941..5178
                     /locus_tag="VIG70_14105"
     CDS             2941..5178
                     /locus_tag="VIG70_14105"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEY8251731.1"
                     /translation="MALRFATLALALAAAAPAGAQTAKPQAKAPDPDADRTVIEADAI
                     EGVSDLEVSARGNAEIRRDDMTIFGETLRYNRETGRAEGDGGVRLQRGADRFFGPHLQ
                     YNILDDTGAFESPSYLLERERTARGTAERIEFLGRDRYRLTNANYTTCRPGQEDWRVE
                     ASEIELDFEEEEGTASGPRLRFFDTTILASPWAVFPLGDRRRSGLLTPYYAQTSSRGF
                     EFGIPYYWNIAPEYDATFTPVYMAKRGAQLKSQGRYLQRSFAGELKFEYLPDDSQFGD
                     SREGVSWQHTHRLPYNTTGQIDYNRVSDDRYFTDLATSVKQLTVGNLPQDAYVTHSGS
                     LGRAPYSAQVRLQQFQTLQDPLAPIVPPYHRLPQINFNTYYNDLAGAFDTALPMEYVN
                     FTHPTLVEGARSSMTPTLAAPLLAPGWFVTPKAGLRYVSYGLTRADPGSPQAPQMSVG
                     WGSLDSGLVYERDTSSFGAGSTQTLEPRLFYVYIPYRNQDSFPIFDTALADFNYAQLF
                     TENRFVGGDRFGDANQATVALTSRILRADGQEMLRATVGQRYYFEAERVGLTPTSPLR
                     TLKDSDILASLGGRVGKSWTYDVTTQYNRYEQRQERFSTAASYRPEPTKVLNASYRFQ
                     RDVLKQVDFSAQWPLYPGWYGVGRYNYSIQDKKLLEGLAGFEYNAGCWVFRAVALRVQ
                     AASQVDTTAFVFQIEFNGIGSIGTDEAVAILKRAVPGYSVSNPADATLAPPSARPRLP
                     FEQVF"
     gene            5183..6466
                     /locus_tag="VIG70_14110"
     CDS             5183..6466
                     /locus_tag="VIG70_14110"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8251732.1"
                     /translation="MKKIFFALLLPVGVAAQGVMPVDRIVAVVNKEVITATELHEAVT
                     AAQRQLRRQGTPLPERGLLERQMLERLILDKAQAQLARDKGIRVDELQLDRAVQRVAQ
                     SNNMSLADFRAALERDRVPFQEWREELREQIVLSRLREREVDDRIQVSDSEIDVFLAQ
                     TQAKPDSRVEYQISHILVRVPEQTTPDRVEVARARAQKALTEARAGADFASLAASYSD
                     APDALQGGALGWRSHDRLPELFSTALAAMKPGDTSEILRSPAGFHVLRLSERRGTALD
                     GAPVAQTRLRHILIRASESMSEGEALRRLTDLRNRIVSGAADFAEMARVHSGDATAAR
                     GGELDWVYAGDTVPDFERAYQELKVGEISPPVRTPFGYHLIQVLERRSADMSPERRRL
                     QARQVLRERKADEAYQEWLRLLRDQVYVELRLEER"
     gene            6409..7368
                     /gene="pdxA"
                     /locus_tag="VIG70_14115"
     CDS             6409..7368
                     /gene="pdxA"
                     /locus_tag="VIG70_14115"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY8251733.1"
                     /translation="MAAPAARPGLRRAATGGAIRRPVVALTSGEPAGIGPELCVMLAR
                     ERFDARVVVIGDRSLLKGYSDVEHVPLRRPPTPGRLDPANSRYVLEVLDRAIRGCLGG
                     EFDAVVTAPVQKSIINDAGIAFTGHTEYLAERAQAPHVVMMLVGGGLRVALATTHLPL
                     AEVPKKINTRDLIETLRVLHADLQKRFHIARPRILVAGLNPHSGESGHFGTEDLAVIA
                     PAVSEARAAGIEATGPLPADTLFVPERLKGADCVLAMYHDQGLPVLKYASFGRGVNVT
                     LGLPFVRTSVDHGTALDIAGSGRADPGSLIEAVKLAIELASPT"
     gene            7340..>7649
                     /locus_tag="VIG70_14120"
     CDS             7340..>7649
                     /locus_tag="VIG70_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HEY8251734.1"
                     /translation="MPSSSHRPRKRFGQHFLHERGVLAKIVDALAPATDDFFVEIGPG
                     EGALTAPLLEKVAKLEAIELDRDLASGLAAKFPPFRLTVHCADALEFDFARFPSGMRL
                     V"
BASE COUNT         1220 a         2659 c         2606 g         1164 t
ORIGIN      
        1 gccgtgagca cctcgcagcc gccatcgctc accagcacgt cgtcctcgat ccggatgccg
       61 atgttgcgca gccgctccgg gatgtcgtct tcggcgcgga tgtagaggcc gggctccacg
      121 gtgagcgtca tcccgggcgc cagcgcgcgc cagctcccct ggcgcttgta gtcgccggcg
      181 tcgtgcacgt cgagcccaag ccagtggccg gtgcggtgca tgtagaagcg cttgtaggtt
      241 tctttttcga ggacctcgtc gaggcttccg ggcacgagct tgaggtcgat gaggccctgc
      301 gccagcacct tgaccgcggc gtcgtgtggc tcgttccagg cgcttccggg gcgcaccgaa
      361 tcgagggcgg caagttgcgc cgccaaaacc acctcgtaga cctcgcgctg cgccgcgctg
      421 aagcagccgt ttacgggata ggtgcgcgtg aggtcggagg catagccgtc gagctcgcag
      481 ccggcatcga tcagcagcag atcgccgtcg cggagcgggg cgtcgttgaa cacgtagtgc
      541 agcacgcagg cgttggcgcc gcccgccacg atcggtgtgt aagcaggagt ttgcgctcca
      601 cttcgccgga actcgtaaag gagctcggcc tcgatctcgt attcggtacg gcccgggcgg
      661 gtgcgctgca tggcgcggcg gtgggcctgt gcggcgatgc gcgccgcgcg ccgcatgacg
      721 ccgagctcgt gcgcgtcctt gacgaggcgc atgtcgtcga cgatggcgcg cacgtcctgc
      781 acgcgctcgg gcgccgcgat cccggcgcgc acctttgcgc gcactgcgtt cagccagcgg
      841 atggcgcgcg cgtcccattc cgggtcggcg cccatcgggt agcagagcgc cggctggttc
      901 tcgagcaggc gcgccatctc ctggtcgagg ttggcgatcg ggtgggcttc gtcgaagccg
      961 aagcgctcgc gcgccgcttc cgggccgtag cggaaaccgt cccagatctc gcgctccggg
     1021 ttgcgctccc ggcaaaacag aatggatttg ttcgaagcca ggaccagcac cgcttccggc
     1081 tcggtgaatc cggtcaggta gtagaagtgg ctgtcgaagc ggtagggata atggctgtcg
     1141 cggttgcgca cgcgctcggg cgcggtcggc accaccgcga cgccggcgcc gatcgcctcg
     1201 gcgaggcgcc gccggcggct ggcgtagacg ggacttgact cagcgatttt cacgtagcga
     1261 tttctccagc tcggcgagcc gctcgatcgt gccgacatcc tgccacaggc cttcgtacag
     1321 ctcgccgccg agcaggccgc gcgccgccgc gtcgtacagc agcggcgcga gcggcgcttt
     1381 ctcgcccggc gtcaccggcc gcaccagcgc gggcgacatg accgcgacgc cggcataagt
     1441 gtagcgcggc gcggcctcgt cgccgacgcg gccgtcgcgc agcgagaagt ctcccttggg
     1501 ccggtggggc ggattgggca cgagtacgag gtgagccagg cgtccgtcaa gcttcacctt
     1561 catcagcttg gagaaatcgc attcgcagta gacgtcggca ttcaccagca ggaagggctc
     1621 gcgcccgagc agcccgagcg cgttggcgat gccgccggcc gtctcgagcc gctcgcgctc
     1681 ccgggagtag gagatgcgca gaccgaatcg cgcgccgtcg ccgatccgct cgacgatccg
     1741 ctcgcccagg tgcgacacgt tgatcacggc ttccgcaaga ccggcgcgcg cgaggcgctc
     1801 caggtgccac gcgatgagcg gcttgccgcc cgcttcgacc agcgccttcg gaatcgcgtc
     1861 ggtcagcggc cgcaggcgct cgccgcgccc ggcggcgagg agcatgacct tcatgcaagc
     1921 tcttccagca atcgcgcgag aggggcgagc gcggcatagc gccgggatac ttctctcgca
     1981 taacccaaga agcgcggcgt gtcctcgagg tacttcggct tgccgtcgcg gtagttgatg
     2041 cgcgcgaaga tgccgagcac cttgaggtgg cgctgcaggc ccatccactc gaacgcgcgc
     2101 cagaactcgg cgaaatccgc ggccaccggc aggccggcgc gcttcgcctt ctcccagtag
     2161 cgcgcgctcc agtcgagcac gcgctcctct tcccagctca ggaaggcgtc gcgcgtgagc
     2221 gaaaccatgt cataggtgat cgggccgagc accgcatcct ggaagtcgag caccccggga
     2281 ttcggttcgc acaccatcag gttgcgcggc atgtagtcgc ggtgcacgta gactacgggc
     2341 tgcgcgagtg cgctttgcac caacagcgcg aagattttct ccagggtccg ctgctgctcg
     2401 gcggaaagcg atttcttcag gtgctttccg acataccagt cggggaacag gcccatctcg
     2461 cggcgcagca gcgcctcgtc gtacggcggc agcgcgccgg ggcgcgtcgc aagctgccag
     2521 cgcagcaggg cgtcggtcgc atcggcgaaa agccgtggtg cctcatttct gccgctatcg
     2581 atgttcagct ctgccagata agtgcgcgtg ccaaggtccg acagcaggag aaatccccgc
     2641 tcgaggtcct gcgcgaggac ttgcggcgcg tgcacaccgg cctcggcgag catgcgggcg
     2701 acgcgcacga acggccggca gtcctccttg tcgggcggcg cgtccatcgc gacgtagctc
     2761 gcgccgtcct cgagcgtcgc gcgccagtag cgccggaagc tcgcgtcctc ggaagccgca
     2821 ctcagcgtga aggaaacgcc gtggaggtgc ggtgcgagcc agcgctccag ggcatcgcgc
     2881 cgcacatcgg cgtgtatcat tcaaaaaatt ctaacaccag ggaaaaagct ttccggctcc
     2941 atggcgctcc gcttcgcgac gctcgcgctc gcgctggccg ctgcggcgcc ggccggcgcg
     3001 cagaccgcaa aaccgcaagc caaggctccc gacccggacg cggaccgcac cgtcatcgaa
     3061 gccgacgcga tcgagggcgt gagcgacctc gaggtcagcg cgcgcggcaa cgcggaaatc
     3121 cgccgcgacg acatgacgat cttcggcgag acgctgcgct acaaccgcga gaccgggcgc
     3181 gccgagggcg acggcggcgt gcgcctgcag cgcggcgccg accgtttctt cggcccgcac
     3241 ctgcagtaca acatcctcga cgacaccggc gcgttcgaaa gcccgagcta tctcctcgag
     3301 cgcgagcgca ccgcgcgcgg caccgccgag cgcatcgagt tcctcgggcg cgaccgctat
     3361 cgcctgacca acgccaacta caccacctgc cggccggggc aggaggactg gcgggtcgag
     3421 gcgagcgaga tcgagctcga cttcgaggag gaggaaggca cggcgagcgg gccgcggttg
     3481 cgctttttcg acaccacgat ccttgcctcg ccgtgggcgg tgttcccgct cggcgaccgg
     3541 cgccgcagcg gcctgctcac gccgtactac gcgcagacca gctcgcgcgg cttcgagttc
     3601 ggcattccgt actactggaa cattgccccg gaatacgacg cgacgttcac gccggtgtac
     3661 atggcgaagc gcggcgcgca gctcaagagc caaggccgct acctgcagcg gtccttcgcg
     3721 ggcgagctca agttcgagta cctgcccgac gacagccagt tcggcgactc gcgcgagggt
     3781 gtctcgtggc agcacacgca ccgtcttccg tacaacacca cagggcagat cgactacaac
     3841 cgggtgtccg acgaccggta tttcaccgac ctcgcgactt cggtgaagca gcttacggtc
     3901 ggcaacctgc cgcaggacgc gtacgttacg cacagcggct cgctcgggcg cgcgccttac
     3961 tcggcgcagg tgcgattgca gcagttccag accctgcagg acccgctggc gccgatcgtg
     4021 ccgccgtacc atcgcctgcc gcagatcaac ttcaacacct attacaacga tctcgccggg
     4081 gcgttcgaca ccgcgctgcc gatggaatac gtgaacttca cgcatccgac gctggtcgag
     4141 ggcgcgcgct cctcgatgac cccgacgctg gcggcgccgt tgctcgcccc gggctggttc
     4201 gtcaccccga aggcggggct gcgctacgtc tcctacggcc tcacgcgcgc cgatcccggc
     4261 tcgccgcagg cgccgcagat gtcggtgggg tgggggagcc tggacagcgg gctggtctac
     4321 gagcgagata ccagcagctt cggcgcaggc tccacgcaga ccctcgagcc gcgcctgttc
     4381 tacgtctaca ttccctaccg caaccaggat tcgttcccga tcttcgacac cgcgctcgcc
     4441 gacttcaact acgcgcagct cttcaccgag aaccgcttcg tgggcggcga ccgcttcggc
     4501 gacgccaacc aggcgaccgt ggcgctgacc tcgcgcatcc tgcgcgccga tggccaggag
     4561 atgctgcggg cgaccgtggg ccagcgctac tacttcgaag cggagcgcgt cgggctcacg
     4621 ccgacctccc cgctgcgcac gttgaaagac tccgacatcc tcgcctcgct cggcgggcgc
     4681 gtcggcaaga gctggaccta cgatgtcacg acgcaataca accgctacga gcagcgccag
     4741 gagcgcttca gcaccgccgc gagctatcgc cccgagccga ccaaggtgtt gaatgcgagc
     4801 tatcgcttcc agcgcgacgt gctgaagcag gtggacttct ccgctcaatg gccgctgtac
     4861 ccggggtggt acggcgtggg gcgctacaat tactccatcc aggacaagaa gctcctggag
     4921 ggcctggccg gcttcgagta caacgccggc tgctgggtgt tccgggcagt cgcgctgcgc
     4981 gtccaggccg cgtcacaggt ggacactacg gcattcgtgt tccagatcga attcaacggc
     5041 atcgggagca tcggcaccga cgaggcggtc gcgatcctga agcgcgcggt gcccgggtac
     5101 tcggtgagca acccggcgga cgccacgctc gcgccgccta gcgcgcgccc gcggctgccg
     5161 ttcgagcagg tgttttgagc gcatgaagaa aatcttcttc gcactgcttc ttcccgtcgg
     5221 cgttgcggcg caaggcgtga tgccggtcga ccgcatcgtg gccgtggtca acaaggaagt
     5281 catcaccgcc accgagctcc acgaggcggt gaccgcggcg cagcgccagc tgcggcggca
     5341 gggaacgccg ctgcccgagc gcggcctgct cgagcgtcag atgctcgagc ggctgatcct
     5401 cgacaaggcg caggcgcagc tcgcgcgcga caagggcatc cgcgtggacg agctgcagct
     5461 cgatcgcgcg gtgcagcgcg tagcgcagag caacaacatg agcctcgccg atttccgcgc
     5521 cgccctcgag cgcgaccggg tgcccttcca ggagtggcgc gaggagctgc gcgagcagat
     5581 cgtgctgtcg cgcctgcgcg agcgcgaggt cgacgaccgc atccaggtga gcgacagcga
     5641 gatcgacgtg tttctcgccc agacgcaggc caagcccgac agccgcgtcg agtaccagat
     5701 ctcccacatc ctggtgcggg tgccggagca gaccaccccg gaccgcgtcg aggtggcgcg
     5761 cgcgcgcgcg cagaaggcgc tcaccgaggc gcgcgccggc gcggatttcg cgagcctcgc
     5821 cgcgagctat tcggatgcgc ccgacgcctt gcagggcggg gcgctcggct ggcgcagcca
     5881 cgaccggctg ccggagctct tttccaccgc cctcgccgcc atgaagcccg gcgacaccag
     5941 cgagatcctg cgcagcccag caggcttcca tgtgctcagg ctgtcggagc ggcgcggcac
     6001 cgcgctcgac ggcgcgccgg tcgcgcagac ccggctgcgg catatcctga tccgggcgag
     6061 cgaatcaatg tccgaaggcg aggcgctgcg ccgcctgacc gacctgcgca accgtatcgt
     6121 cagcggggcc gccgatttcg cggagatggc gcgcgtgcat tcgggcgacg ccaccgcggc
     6181 gcgcggcggc gagctcgact gggtgtacgc gggcgacacc gtgcccgatt tcgagcgcgc
     6241 ctaccaggag ctgaaggtgg gcgagatcag tccgccggtg cgcaccccct tcggctacca
     6301 cctgatccag gtgctggagc ggcgctcggc cgacatgagc ccggagcggc gccggttgca
     6361 ggcacgtcag gtgctgcgcg agcgcaaggc ggacgaggcc taccaggaat ggctgcgcct
     6421 gctgcgcgac caggtctacg tcgagctgcg actggaggag cgataagacg cccggtcgtc
     6481 gcgctgacct ccggcgagcc cgccggcatc gggccggagc tgtgcgtgat gctggcgcgc
     6541 gagcgattcg acgcccgggt ggtcgtgatt ggcgatcgca gcctcctgaa aggctattcc
     6601 gacgtggagc atgtcccgct gcggcggccg ccgaccccgg gtcggctcga cccggccaac
     6661 agccgctacg tgctcgaggt cctcgaccgc gccattcgcg gctgcctggg aggcgaattc
     6721 gacgcggtgg tgaccgcgcc ggtgcagaaa agcatcatca acgacgccgg catcgcgttc
     6781 accgggcaca cggaatatct cgccgagcgc gcgcaggcgc cgcacgtggt gatgatgctg
     6841 gtcggcggcg gcctgcgcgt ggcgctcgcc accacgcacc ttcctttggc tgaagttccc
     6901 aagaaaataa acacccggga cttgatcgaa acgctgcgtg tcctgcacgc cgatctgcag
     6961 aagcgcttcc acatcgcgcg gccgcgcatc ctggtcgccg gcctcaatcc gcacagcggc
     7021 gagtcggggc acttcggcac cgaggacctg gccgtcatcg cccccgccgt gagcgaggcg
     7081 cgagccgccg gcatagaggc gaccgggccg ctgccggccg acacgctttt cgtgccggag
     7141 cggctgaaag gcgccgactg cgtgctcgcg atgtaccacg accagggcct gccggtgctc
     7201 aagtacgcct cgttcggccg gggcgtgaac gtgacgctcg ggctcccctt cgtgcgcacc
     7261 tcggtcgatc acggcaccgc gctcgacatc gcgggcagcg gccgcgccga cccggggagc
     7321 ctgatcgagg cggtgaaact tgccatcgag ctcgcatcgc ccacgtaaac ggtttggtca
     7381 gcacttcctg cacgagcggg gagttctggc aaagatcgtc gatgcgctcg cgccggcgac
     7441 ggatgacttc ttcgttgaaa tcggcccggg cgaaggcgcg ctgaccgcgc cgctgctcga
     7501 aaaggtagcc aagctcgagg cgatcgagct cgatcgcgac ctcgcctctg gcctggcggc
     7561 gaagttcccg cccttcaggc tcacggtgca ctgcgccgac gcgctcgagt tcgacttcgc
     7621 gcgcttcccg tccgggatgc ggctcgtcg
//
LOCUS       DATBSZ010000227         7232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_452263, whole genome shotgun sequence.
ACCESSION   DATBSZ010000227 DATBSZ010000000
VERSION     DATBSZ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452263"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1803
                     /locus_tag="VIG70_14125"
     CDS             <1..1803
                     /locus_tag="VIG70_14125"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8251735.1"
                     /translation="RSLPLLAAAVVAALALELAVLQWMKPLENRLLDSFVKRHAAALA
                     PDPDVVLVSIDEKSLAQMEEIAGRFPWPRAVYGTLIDGLAPQRPRAIVFDILFSEADK
                     VRPESDQAFVEAALRNSSVNIYYPMVRLDTPHGARAGQLAPLLGLVRGAGADPDARIA
                     VVPPLVLPQKLWRTGTINFLPDDDGVGRRYELRRRIGGWDLPSLPARVAMDLGFPVPD
                     ANDLLLAWRSGAEQLPRVSFSDLFEDFERAKRRRPADEFTGKIVVVGASAAGLGDARV
                     TPLSHTQPGSEILATAIENLKNARAMRNAPPWWPAAIGVALVLLLYLAFHKGIDMRAS
                     GAALAVVTALLVAGQWLLVGWLTLLPLLTPLAAAWTFYAAAALAEYLRERHARRAAVA
                     QFSRFVNPHVVKELVEGSGIQTGRREVTLLFSDIRGFTTLSETRKPEEVIALLNRYFS
                     LQVDVVVRHGGSLDKYIGDCIMAIWGAPLDDPEHARNAVACALDMADVLKAFKRELGA
                     EDLDFDVGIGLHSGPAVVGLMGSPKRLEYTAIGDTVNLASRIEGLTKDAKRRILVSRD
                     TADRCGGAYDFVSCGTFPVKGRAQPVELLEPRRK"
     gene            1803..2276
                     /locus_tag="VIG70_14130"
     CDS             1803..2276
                     /locus_tag="VIG70_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY8251736.1"
                     /translation="MRLLALLLLVPMAVLAEPATVIRATELKQEPASDSATLAALAEN
                     AAVEVLERKSGWTRVKAQPGEGWVRMLALRFGGTASAKPGATGVTQLFNVARTGSSGT
                     QVTTGVRGLDAEQIANAQPNPAELAKLEKFAADRDAAAGFAAQGKLSATAVAYPK"
     gene            2273..3118
                     /locus_tag="VIG70_14135"
     CDS             2273..3118
                     /locus_tag="VIG70_14135"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY8251737.1"
                     /translation="MRVFLLLAGLAFAAAAHAQFRLPSLDINKMVDTVKNVGKAVKDI
                     DEPEEIAIGKDVASRLLGAAPLVADARMQRYVNHVGRWVAAQTERADLPWHFGVLDSP
                     NVNAFAVPGGTIFITRGLLDRMRNEAELAGVLGHEVAHVLRKHHLKAIQKGAQSALAG
                     DAMSAALRDRAGPARDKLIAFGTEMYSRGLDKSDELEADRLGVVIAARAGYDAYGLPS
                     VLQTLQAMNAQDSALALMFKTHPAPGERLDALGEKMQPVLDAYSRQPQLGERFVQQIK
                     TQATR"
     gene            complement(3108..4121)
                     /locus_tag="VIG70_14140"
     CDS             complement(3108..4121)
                     /locus_tag="VIG70_14140"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEY8251738.1"
                     /translation="MDTLTHALSGALLARATAPKDASPRSLPRRIAAGFFACAAPDLD
                     FVIGFVGPVEYLLTHRGVTHSLILLPAWALLYSWILAKILREPGGWRALYGVTALALL
                     VHIVGDLITSFGTMILAPFSDWRAALGTTFIIDLWFSGIILAGLIFSALFSRSRIPAV
                     AAAVALAGYVGFQYLQKQRALDFGAEYAGSMAIRDARITVHPRPVSPFNWTVFVSDEE
                     AHRFAHVNLVRREPRRYRPGDGFIARIDSPYLPVEQAIWVKRSRYGETDSAMIRDAWN
                     SEGLAFFRWFAALPAFDGQSDGSTCVWFTDLRFLTPGRETMPFRYRVCRSRPGAPWRL
                     AIS"
     gene            complement(4192..5688)
                     /locus_tag="VIG70_14145"
     CDS             complement(4192..5688)
                     /locus_tag="VIG70_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8251739.1"
                     /translation="MSCAERLVELFGSQAEVARAFQLDRAVVHNWVKTGYVPARWAGE
                     VERVTQGRISATEVVNEASAKNPVRVKSRPENAPFGVFSEENMSQFAPTKRIQSFHPP
                     QRTLMGPGPTEIHPRVLTTMSQPAIGYLDPVFVEMMEELKALLRYVYQTKNALTFPLS
                     GPGSVGMEYCFVNMVAPGDKVVVCQNGVFGGRMLENAARAGGNPVVVEDKWGEPVDPQ
                     KLEDALKKNKDAKVVGFVHAETSTGCQSDAKLLAQIAHKHGAMVIVDAVTSLAGTPVK
                     VDEWELDAIYSASQKCLSCTPGLSPVSFSERVVEHVKKRKDKIHSWFMDMNLLFNYWT
                     AGTRTYHHTAPTNSLFALHEALLLIREEGLENSWARHTRHHLALKAGLEAMGLRFLVA
                     EKHQLPQMNAVVCPEGVNEEEVRRTLLAEFGLEIGAGLGPLKGKIWRFGLMGYSCRPD
                     NVMLCLSALGSVLSDMGVPVHVGDAEAAAHGAYAQMHVKDAQRKKRAA"
     gene            5774..6169
                     /locus_tag="VIG70_14150"
     CDS             5774..6169
                     /locus_tag="VIG70_14150"
                     /inference="COORDINATES: protein motif:HMM:NF042861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HEY8251740.1"
                     /translation="MKEPAPKVDLRSRASRERLAKMVVSLLEHWKLPLNDQASLLGLS
                     AQSRSTIARYRRGEPFADSADLLARAGHVLGIHKALRILFPQDRDLAYRWVSAPNRRF
                     GERAPLEIMKQGFEGLIAVRRYLDFERGR"
     gene            6170..6871
                     /locus_tag="VIG70_14155"
     CDS             6170..6871
                     /locus_tag="VIG70_14155"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEY8251741.1"
                     /translation="MYENLLREVADYHGDLVRNIQTIRVSQDLFDDLSPDHGDQAVAI
                     AAEAAGRIPSDSPLITRPFDYGTVITFPFANFNGQGTRFSDGLRYGVWYGSLQLETTV
                     YETAYHRHRFLMDSFPSLNRDIRLERRVFQVRCEAILIDLQGKEAAEPRLVDRASYAY
                     TQPLGAYLQAQGTNGLLVKSARCEGTNAAIFRPAVLSHVRDLCHLTYTMNPAHDTATI
                     ERTPGETWLRIKIPV"
     gene            6868..>7232
                     /locus_tag="VIG70_14160"
     CDS             6868..>7232
                     /locus_tag="VIG70_14160"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY8251742.1"
                     /translation="MKPDHPVTFKPETAADAWLGLLAARGIEYLFANGGTDFAPVVEA
                     YAKGQKLGWRLPEIVIVPHENMGVAMAHGYAMVTGRPQAMMVHVGVGTANSLNGLINA
                     SRQNVPILFTAGRTPITESG"
BASE COUNT         1187 a         2508 c         2401 g         1136 t
ORIGIN      
        1 cgcagccttc ccctgctcgc ggctgcggtg gtcgcggcgc ttgcgctcga gctcgcggtc
       61 ctgcagtgga tgaagccgct cgagaaccgg ctgctcgaca gcttcgtcaa gcgccacgcc
      121 gccgcgcttg cgcccgaccc ggacgtggtg ctcgtcagca tcgacgagaa gagcctggcg
      181 cagatggagg agatcgccgg ccgctttccc tggccgcgcg cggtatacgg cacgctgatc
      241 gacgggctcg cgccgcagcg gccgcgcgcc atcgtgttcg acatcctgtt ctccgaggcg
      301 gacaaggtgc gcccggagag cgaccaggcc ttcgtcgagg cggcgctgcg caacagcagc
      361 gtcaacatct attacccgat ggtccggctc gatacgccgc acggcgcgcg cgccggccag
      421 ctcgcgccgc tgctcggcct ggtgcgcggc gccggcgccg acccggatgc ccgcatcgcg
      481 gtcgttccgc cgctcgtgct gccgcagaag ctgtggcgca cgggcacgat caacttcctc
      541 cccgacgacg acggggtcgg ccggcgctac gagctgcgca ggcgcatcgg gggctgggac
      601 ctgccctccc tgccggcccg ggtggccatg gacctcggtt ttcccgtgcc cgatgcgaac
      661 gatctgctgc tcgcctggcg cagcggcgcg gagcagctgc cccgcgtctc cttcagcgac
      721 ctgttcgagg atttcgagcg cgcgaagcgc cggcggccgg ccgatgagtt caccggcaag
      781 atcgtggtgg tcggcgcgag cgccgccggc ctgggcgacg cgcgcgtgac gccgctttcg
      841 cacacgcagc cgggctcgga gatcctcgcg accgcgatcg agaacctcaa gaacgctcgc
      901 gcgatgcgca acgcgccgcc gtggtggccg gccgcgatcg gcgtcgcgct cgtgctgctc
      961 ctctatctcg ccttccacaa gggaatcgac atgcgggcga gcggcgccgc gctcgccgtc
     1021 gtcacggcgc tgctcgtcgc cggccaatgg ctgctggtcg gctggctgac gctgctgccc
     1081 ctgctgaccc cgcttgccgc ggcgtggacg ttctacgccg cggccgctct cgccgaatac
     1141 ctgcgcgagc gccacgcgcg ccgcgccgcg gtggcgcagt tctcccgctt cgtgaatccg
     1201 cacgtcgtga aggagctggt cgagggcagc gggatccaga ccggccggcg cgaagtgacg
     1261 ctgctcttct ccgacatccg cggctttacc acgctctcgg agacgcgcaa gcccgaagag
     1321 gtgatcgcgc tcctcaaccg ctacttttcc ctgcaggtcg acgtggtcgt ccgccacggc
     1381 ggctcgctcg acaagtacat cggcgactgc atcatggcga tctggggcgc gccgctcgac
     1441 gaccccgagc acgcccgcaa cgccgtcgcg tgcgcgctcg acatggccga cgtgctgaaa
     1501 gcgttcaagc gcgagctcgg cgccgaggat ctcgacttcg acgtcggtat cggcttgcat
     1561 tccgggccgg cggtggtcgg gctgatgggg tcgccaaagc gcctcgagta caccgccatc
     1621 ggcgacaccg tgaacctcgc gagccgcatc gagggcctca ccaaggacgc aaagcgcagg
     1681 atccttgtgt cgcgcgacac agccgaccgc tgcggcggcg cgtacgattt cgtatcatgc
     1741 ggaacgtttc ccgtgaaagg ccgcgcccag cccgtagagt tactcgagcc aagaagaaag
     1801 tgatgagact cctcgctctg ctgctccttg tcccgatggc cgtcctggcg gaaccggcga
     1861 ccgtgatccg cgcgaccgag ctcaagcagg aaccggccag cgattccgcg accctcgccg
     1921 cgctcgccga gaacgccgcc gtggaagtgc tggagcgcaa gagcggctgg acccgcgtca
     1981 aggcgcagcc cggcgagggc tgggtgcgca tgctggcgct gcgcttcgga ggcaccgcct
     2041 ccgccaagcc gggcgcgacg ggcgtgaccc agctcttcaa cgtcgcgcgc accggttcga
     2101 gcggcacgca ggtgacgacc ggcgtgcgcg gcctggatgc cgagcagatc gccaacgcgc
     2161 agcccaatcc cgccgagctt gccaagctgg agaagttcgc cgccgaccgg gatgcggctg
     2221 caggcttcgc cgcgcaaggc aagctgtccg cgacggcggt ggcctatccc aaatgagagt
     2281 cttccttctt ctcgccgggc tcgccttcgc cgccgcggcg cacgcgcaat tccgcctgcc
     2341 gagcctcgac atcaacaaga tggtcgacac ggtcaagaat gtcggcaagg cggtcaagga
     2401 catcgacgag cccgaggaga tcgcgatcgg caaggacgtc gcttcgcggc ttctcggcgc
     2461 tgcgcccctc gtcgccgatg cccggatgca gcgctacgtc aatcacgtcg gccgctgggt
     2521 cgcggcgcag accgagcggg cggacctgcc ctggcacttc ggcgttctcg actcgcccaa
     2581 cgtgaacgcg ttcgcggttc ccggcggcac gatcttcatc acccgaggcc tgctcgacag
     2641 gatgagaaac gaggccgagc tcgccggcgt gctggggcac gaggtcgcgc acgtcctgcg
     2701 caagcaccat ctcaaggcaa tccagaaagg cgcgcagagc gcgctcgccg gcgacgcgat
     2761 gtcggcggcg ctcagggacc gcgccgggcc ggcgcgcgac aagctgatcg ccttcggcac
     2821 cgagatgtac agccggggcc tcgacaagtc cgacgagctc gaggccgacc gcctgggcgt
     2881 ggtgatcgca gcgcgcgccg gctatgacgc ctacggcctg ccctcggtgc tgcagacgct
     2941 gcaggcgatg aacgcccagg actccgcgct cgcgctgatg ttcaaaaccc atccggcgcc
     3001 aggcgagcgc ctcgatgcgc tcggcgagaa gatgcagccg gtgctcgacg cctactcgcg
     3061 gcagccgcag ctcggcgagc gcttcgtcca gcagatcaag actcaggcta cgagatagcg
     3121 aggcgccagg gggcgcccgg gcggctgcgg cacacgcggt agcggaacgg catcgtttcg
     3181 cgccccggcg tgagaaacct caggtcggtg aaccagacgc acgtcgagcc gtcgctctgg
     3241 ccgtcgaacg ccggcagggc ggcaaaccag cggaagaagg cgagcccctc cgagttccag
     3301 gcgtcccgga tcatcgcgct atccgtctcg ccatagcgcg agcgcttgac ccagatcgcc
     3361 tgctccaccg gcaggtaggg agaatcgatc ctggcgatga agccgtcgcc gggccggtag
     3421 cgccgcggct ccctgcgcac gagattcacg tgcgcgaagc gatgcgcttc ttcgtcgctc
     3481 acgaacaccg tccagttgaa gggtgagacc ggccgcggat gcaccgtgat cctggcatcc
     3541 cgaattgcca tggaacctgc gtactccgcc ccaaaatcca gggcgcgctg cttttgcagg
     3601 tattgaaagc cgacgtatcc cgccagcgcg acggctgcgg ccaccgccgg aatcctgctt
     3661 ctggaaaaca atgcggaaaa gatcaggccg gcgaggatga tgccgctgaa ccagaggtcg
     3721 atgatgaagg tcgtgccgag cgccgcgcgc cagtcggaaa agggcgcgag gatcatggtg
     3781 ccgaagctgg tgatgagatc gcccacgatg tgcacgagca gcgcgagcgc ggtgacgccg
     3841 tagagcgcgc gccagccgcc gggctcgcgc aggatcttgg ccaggatcca ggaatacagg
     3901 agcgcccacg ccggcagcag gatgagcgag tgcgtgacgc cgcggtgcgt gagcaggtat
     3961 tcgaccggtc cgacgaagcc gatgacgaaa tcgaggtccg gggcagcgca ggcgaagaac
     4021 ccggcggcta tgcgccgcgg aagcgagcgc ggcgacgcgt ccttcggcgc agttgcgcgc
     4081 gcgaggagcg cgccggacag agcgtgcgtt aaggtgtcca aacgaaatca gggacagacc
     4141 acgttttccc cattgtgcga gaaaaacgtg gtccgtccct gattcaggtt cttaagccgc
     4201 ccgcttcttg cgttgtgcgt ctttcacgtg catctgggca taggccccgt gcgcggccgc
     4261 ctcggcatcg ccgacgtgca ccggcacgcc catgtcggaa agcaccgagc cgagcgccga
     4321 caggcaaagc atgacgttgt ccgggcggca gctgtagccc atcaggccga agcgccagat
     4381 cttgcccttg agcggcccca ggccggcgcc gatctcgagg ccgaactcgg cgaggagcgt
     4441 gcggcgcact tcttcctcgt tcaccccttc ggggcagacc acggcgttca tctgcggcag
     4501 ctggtgcttt tccgccacca ggaaccgcag ccccatcgcc tccagcccgg ccttgagcgc
     4561 cagatggtgg cgcgtgtggc gcgcccacga gttttccagc ccttcctcgc ggatgaggag
     4621 cagcgcctcg tgcagcgcga acagcgagtt ggtcggcgcc gtgtggtggt aggtgcgcgt
     4681 gccggcggtc cagtagttga acaggaggtt catgtccatg aaccagctgt ggatcttgtc
     4741 cttcctcttt ttcacgtgct cgaccacgcg ctcggagaac gacaccggcg acagcccggg
     4801 cgtgcaggag aggcacttct ggctcgccga gtagatggca tcgagctccc actcgtcgac
     4861 cttcaccggc gtcccggcga gcgaggtcac cgcgtccacg atcaccatcg cgccgtgctt
     4921 gtgcgcgatc tgcgcgagga gcttcgcgtc cgactggcag ccggtggaag tctccgcgtg
     4981 cacgaacccc accaccttgg cgtccttgtt cttcttcagg gcgtcctcga gcttctgcgg
     5041 gtcgaccggc tcgccccact tgtcctcgac caccaccggg ttgccgcccg cccgcgccgc
     5101 gttttccagc atcctgccgc cgaacacgcc gttctggcag accacgacct tgtcgcccgg
     5161 ggcgaccatg ttgacgaagc agtactccat gccgaccgag cccggccccg acagcgggaa
     5221 ggtgagcgcg ttcttggtct ggtacacgta gcgcagcagc gccttcagct cctccatcat
     5281 ctcgacgaag accgggtcga ggtagccgat ggcgggctgg ctcatcgtcg tgagcacgcg
     5341 cggatggatc tcggtcggcc ccgggcccat cagcgtgcgc tgcggcgggt ggaaggactg
     5401 gatgcgcttc gtgggagcaa actggctcat gttttcctcg gagaaaacgc caaatggtgc
     5461 gttctcgggg cgggatttca cccgcaccgg attttttgcg ctggcttcgt tgacgacttc
     5521 cgtcgcgctg atgcggccct gggtgacgcg ctcgacctcg ccggcccagc gcgccggcac
     5581 gtagccggtc ttgacccagt tgtggaccac ggcgcggtcg agctggaacg cccgggcgac
     5641 ctcggcctgg ctgccgaaaa gctccactag gcgttctgcg cagctcatgc gcaaagccta
     5701 ccctgtcaaa gtaggtttga caatccgcca tccggtctgt accaattagt actgataacg
     5761 tactatctcg tacatgaagg aacccgcgcc gaaggtcgat ctgcgaagcc gggcgagccg
     5821 ggagcggctc gcgaagatgg tggtttctct cctcgagcac tggaaattgc cgctcaacga
     5881 ccaggcgtcg ctgctcggcc tgtcggcgca gtcgcgctcc acgatcgcgc gctaccgccg
     5941 cggcgagccc ttcgccgaca gcgccgacct gctggcgcgc gccggccacg tgctcggcat
     6001 ccacaaggcg ctgcgcatcc tgttcccgca ggaccgcgat ctcgcctacc gctgggtaag
     6061 cgcgccgaac cggcgcttcg gcgagcgcgc tccgcttgaa atcatgaagc agggcttcga
     6121 gggcctgatc gccgtgcggc gctacctgga tttcgagcgc ggccggtagg tgtacgaaaa
     6181 cctgctgcgc gaggttgccg actaccacgg cgacctggtg cgcaatatcc agacgatccg
     6241 ggtttcgcag gacctgttcg acgacctctc gcccgaccac ggcgaccagg ccgtagccat
     6301 cgccgccgaa gcggcagggc gaattccgag cgactcgccc ctcatcaccc gcccgttcga
     6361 ctacggcacg gtgatcacgt ttccgttcgc caatttcaac gggcaaggca cccggttctc
     6421 ggacggcctg cgctacggcg tctggtacgg gtcgctccag ctcgagacca cggtgtacga
     6481 aacggcgtac caccggcacc gctttctcat ggacagcttc ccatcgttga atagagacat
     6541 tcggctggag cgacgcgtgt tccaggtgcg ctgcgaagcg atcctcatcg acctgcaggg
     6601 caaggaggcg gccgagccgc ggctcgtgga ccgcgcgagc tacgcgtata cgcaaccgct
     6661 gggggcctac ctgcaggcgc agggcaccaa cggcctgctc gtgaagtccg cgcgctgcga
     6721 aggcacgaac gcggcaatat tccggcctgc ggtgctgtcg cacgtgcgcg acctttgcca
     6781 tctgacgtac acgatgaacc ccgcgcacga tacggccacc atcgagcgta cgcccggcga
     6841 aacctggcta aggattaaga ttcccgtatg aagcccgacc atcccgtgac cttcaaaccc
     6901 gaaacggcag ccgacgcctg gctcggactg ctcgccgcgc gcggcatcga gtatctcttc
     6961 gccaacggcg gcaccgactt cgcccccgtg gtcgaagcct acgccaaggg gcagaagctc
     7021 ggctggcgcc tgcccgagat cgtgatcgtg ccgcacgaga acatgggtgt cgccatggcg
     7081 cacggctacg ccatggtgac cgggcggccg caggcgatga tggtgcacgt cggcgtcggc
     7141 accgcgaact cgctcaacgg cctgatcaac gcctcgcggc agaacgtgcc gatcctgttc
     7201 accgccgggc gcacgccgat caccgagtcc gg
//
LOCUS       DATBSZ010000228        24940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2159112, whole genome shotgun sequence.
ACCESSION   DATBSZ010000228 DATBSZ010000000
VERSION     DATBSZ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 24940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24940
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2159112"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VIG70_14165"
     CDS             <1..1109
                     /locus_tag="VIG70_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEY8251743.1"
                     /translation="NPTVAVLEERVAAIENGRAALACATGQAAEATAILTLCSAGDHI
                     VSASTLYGGTHTLLHVNLKKLGIETTFVNPDEPENFRKAIQKNTKLLYAETLGNPLIN
                     IVDIEALAKIAHEAEIPLVLDNTVPSPYLCRPLEWGADIVVHSATKYIGGHGTTMGGV
                     IVESGKFNWGNGKFPEFTSPSPGYHGVIFHETFGDFGYTMKARMEIMRTFGPALAPLN
                     AWLLLQGLETLHVRMERHCANALAVARFLQGHPRVAWVNYPGLPDNKYHALAKKYLPK
                     GASGLLNFGVRGGAKAGERFIEAAQFMSHLANIGDAKTLVIHPASTTHRQMEEADQLK
                     AGVTPDMIRISVGIETLDDLLWDLDQALTEAGKA"
     gene            1106..2161
                     /locus_tag="VIG70_14170"
     CDS             1106..2161
                     /locus_tag="VIG70_14170"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEY8251744.1"
                     /translation="MISYQIIEHGKPLQRVALETPKPQGTEVLVRITRAGVCHSDVHI
                     WDGYFDLGGGKRFYVKERGCIPPFTLGHEPFGVIEALGPRARGVRVGQKKIVYPWIGC
                     GKCAVCKAGQDNYCVSGIRFLGVSRPGAYSTHVLVPDPKYLVDAAGIEEGFAATLACS
                     GLTAYSAAAKLPPIGAQDRVAVLGCGGLGLIGISVLRAKGVKHIVACDIDDAKLAAAS
                     KQGAKTTLNTKAQDAMQQLQGIAAAIDFVGSPATAALGIGALRKGGRYVICGLYGGEL
                     VHPLPPIAQRAIGIVGSYVGNLQELKEVVALAKKRKIKAAPVETRAAEAASAALEDLK
                     AGRVLGRVVLDFESVTA"
     gene            2158..4617
                     /locus_tag="VIG70_14175"
     CDS             2158..4617
                     /locus_tag="VIG70_14175"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY8251745.1"
                     /translation="MSFRSKGSPDITSQMARDQLAAKAKAVSAQRFVWPAPPYYEFSD
                     PSRHSDECMLVFRDGAKTSGTMLDFRPDEAQLKFRSHDSGDDLNVAFSSLLRVQLLQP
                     VTLRRQPMPIDDSDALFAASERQPFAIGLANGKTFLGETVGAVHALCGLFLFPPEQEG
                     YVTRWFVPVGATRSASIGKPLGEMLVDENLASAEAVDAALSKQQAMRARRFGDYLTEH
                     QIVSPEQLSTALKQQRAQPMQKIGETLVDLGYLTEDELQEALAIEARDRSVPLGQILA
                     DMGVVDAEVVNALMARKLGVPFVNLKNFHIAPEVLKRVPAAVAHRFQTLPVAEAETSL
                     VVAVENPTDMAKLEGLRFVAGMKLLPVMASGDDIRAALERCYGPTQAPLSSVRKTPDL
                     AIGELTHRLSVETADADLDETQSAAQDSALVQLVNKMIVDAIEQQASDIHVETNAAGR
                     NLRVRFRKDGVLVPYLDLPSKFRKALVSRIKIMCHLDITERRKPQDGKIEFRRFGPLD
                     VDLRVATIPTAGGLEDVVMRVLAAATPVPLAELGFDPQTLERIRHLITRPHGLFLACG
                     PTGSGKTTTLHSLLGSINTAETKIWTAEDPIEIAQPGLRQVQVNPKIGWTFAAAMRSF
                     MRADPDIIMVGEMRDAETAKMGIEASLTGHLVLSTLHTNSAPESVIRLLDLGMDPFNF
                     ADALLGVLAQRLVRRLCPDCKTGYAASTPELEELATEYCGDHGDPGKVLQGWRSQPRG
                     IVLYRAKGCAKCDRTGYKGRMGIYELLVANAAIKRQVQTRAPVQEIAAAATANGMRTL
                     KQDGIDKVIRGLTDLQQVRTV"
     gene            4662..6263
                     /locus_tag="VIG70_14180"
     CDS             4662..6263
                     /locus_tag="VIG70_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEY8251746.1"
                     /translation="MKMEHPPTFSWANPYAWPRKPLLAANVVSTSQPLAAQAGLQMLA
                     AGGSAVDAILATAITLTLVEPVSNGIGSDAYAIVWDGSRLHGLNASGRSPAAWSPEYF
                     AGQKAMPVRGWNSVSVPGCVSAWMEMHKRWGKLPFGKLFERAIQYGREGFLVSPTIAG
                     QWEKQVPELKNQPGFAEAFLPDGRPPRPGQRFRFPAHANVLEKIASTRGEAFYRGEFA
                     ERLEAHAKKHGSAMRAADMAAHKSDWVDTLTMDYRGYTLHEIPPNGQGIVALMALGML
                     EHFDLRSHPVDGPDSVHLQIEAQKLAFADAWRYVADVDHMKDVRPSSLLDKSYLKERA
                     RLIDPKRAQDFGPGKPPRGGTVYLAAADASGMMVSFIQSNYMGFGSGVVVDGISLQNR
                     AATFTLQAGHPNCVGPGKRPYQTIIPGFVTREGAPVMSFGVMGGTMQPQGHAQVMVRI
                     ADYGQSPQAACDGARFRFVQNMDVSVEDGRFPPATLEGLQRRGHRIVTIDDYNQFGSA
                     QLIWKLDGGYFVASDPRRDGQAVGF"
     gene            6303..6920
                     /locus_tag="VIG70_14185"
     CDS             6303..6920
                     /locus_tag="VIG70_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY8251747.1"
                     /translation="MLKIWGRVNSVNVKKALWVMEELGLKYERIDAGMEHGVVKTPQY
                     MKMNPNSLVPTIDDDGFVLWESHAIVRYLAAKHGAGTLWPADLRQRADSDRWMDWAYT
                     FQAQFRTVFWGLVRTPPEKRDLKAIEEARKKCGELLAIPDQALSHKPYFAGNSLTIGD
                     IPLGCHVHLWMRLPIERPAHPNLQRWFDRLCARAAFKKVVDIPLS"
     gene            complement(6889..7893)
                     /locus_tag="VIG70_14190"
     CDS             complement(6889..7893)
                     /locus_tag="VIG70_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEY8251748.1"
                     /translation="MRWFVGILLFVLIAGCQSFDYYTQAIGGHLKMMARARPVSDWIA
                     DPATPPELKQRLETARRIREFASKSLKLPDNNSYLTYADLDRSYVVWNVFAAPEFSVE
                     PKKECFPFTGCVSYRGFYSEDDARRHAVRLREQGYDVYVGGVPAYSTLGWFDDPLLST
                     FIRYSDAQLARLVFHELAHQLVYARDDTTFNESFAVTVEQEGVKRWLESEKRAGELAG
                     FHAAQARQRELAERVSQARERLAGVYRMDLAREAMLEHKRGEWDRLRASYPALPAEPN
                     NAFLLSIAVYTELVPAFERLLAESGDLEDFYRRVKKLAAAKQPERDAYFRKEGYPRPS
                     "
     gene            complement(7890..8495)
                     /locus_tag="VIG70_14195"
     CDS             complement(7890..8495)
                     /locus_tag="VIG70_14195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8251749.1"
                     /translation="MKARVVLAAGLWMPAVAMALLSSRLKRRGYEVHVFDYRGRSPHD
                     ANVEALADFAPGKTLFVGHSLGGVLILDMLMRHPEIPADAAVLLGAPVRGCLAGRRLG
                     RAGLGRWMMGACRALWEERAARWTRAAPLGVVAGTLPLGLGRALGGALPGPNDGVVCV
                     EETSVDGMTGRALVRQGHSMLIVSGEVGGLVERFLASGRFA"
     gene            complement(8492..8920)
                     /locus_tag="VIG70_14200"
     CDS             complement(8492..8920)
                     /locus_tag="VIG70_14200"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY8251750.1"
                     /translation="MPPIPEGFRKIERLSPFNALVGPLYERRDGETVSIGLAIEDKHA
                     NSRGICHGGVLATLADLALGYAMLAKSGEIGGQRHGSFVTAHLAVDYAGAARPGDWIE
                     SKVEIQRVGSRLAFANCYLISNEKPIVRASAIFARDGKGE"
     gene            complement(8904..10052)
                     /locus_tag="VIG70_14205"
     CDS             complement(8904..10052)
                     /locus_tag="VIG70_14205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8251751.1"
                     /translation="MSTAVYCAPVMAPAAAPALGVTPAAVGYFVTVVYLGSMIGTVTA
                     GGWVGRFGPILVSQAGLLLCLAGLAVAASGSLPAVLVGALLLGLGYGPATPASSVILA
                     RTAPPHLLALMFSIKQTGVPLGTAIAGAAVPLMVLALGWQAAALAIGIACALCAAALA
                     PIRGRYDAGRNPAAPASIRSAFAPVALIVRDRTLLELSLVSFIYGGMQITLLAYLVTF
                     LVESFSLSLVLAGMVMAASQLTSVAARIGWGLFADRIATRRATLGLLGIGMGLTAITA
                     LAASPAWPLWALFAFAMAYGATAVGWNGVFLAEIARRAPRDRISDATGGSAFFTFLGV
                     VVTPPLFHLVLVLTGSYGATYAIFGVPALAAGLRLLLTAKIARDATHS"
     gene            complement(10097..10975)
                     /locus_tag="VIG70_14210"
     CDS             complement(10097..10975)
                     /locus_tag="VIG70_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY8251752.1"
                     /translation="MNRSFLFAPGNVARRVEKALTLDADAVIVDLEDSVALAEKAATR
                     KAVAAALERPRRPRGYVRVNAPSSVFCFDDLTEVLHRGVEGVVLPKVESAADLHAIDW
                     VIWNLERSRGIAEGSIDLVPQIETAAGVQRIDRVLQARSLRPYRGPWRVKRVAFGAAD
                     YAHELGLTVGLEETELAEVRARVVLASRAAGLEGPIDSPWFHFKEAGGFARSLDRSRR
                     GGFQGRLCVHPDQIAPVNAAYLPSGEELARAERIVAAFKQAEAKGEAAVQIDGEMVDY
                     PVAYRAQALLDRVRKN"
     gene            complement(10972..12138)
                     /locus_tag="VIG70_14215"
     CDS             complement(10972..12138)
                     /locus_tag="VIG70_14215"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251753.1"
                     /translation="MNTIRLLFVWLAGLCVALAQERPAPAHLTITVGAAISQTGAHAE
                     LAADYLKGLQLWREQMNESGGLLGRRVELRVVDDNSQAVRARELYAELLRDPKVDLLI
                     GPYGSAATLLAAAEAERARRVMVNGAGPAAVVHSRPGRFVFQTAVPYSVYGLGVLPVA
                     ETAELRRLFILARDDAASREMAEATRNTALNGFVPGEVELYRPGTVDFAAQVAKARAA
                     KAEAWIAFGDVRDAAEMVKTFKRLDYAPQLFFAGAASHPRFIALLGQDAEFSLGVVDF
                     DPRVGDAEARAFTKAFTVRWTVPPQLAAAEGYTAGTVLAAAVRSAGNLDQERLRAALA
                     ELELPTALGKYRVAPENGAQIGFRPALVQIRRGRPETGTPLLPYPQWSERALIK"
     gene            12141..13487
                     /locus_tag="VIG70_14220"
     CDS             12141..13487
                     /locus_tag="VIG70_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8251754.1"
                     /translation="MTLQRRLGDIVGAAHVLTAPADTKPYFTDWRKQYSAPAECVVRP
                     ASTEEVARVVALCARESVAVVPQGGNTGLVGGSVPTGARREIVLALGRMNRIRALDAL
                     NDTITVEAGCVLAAVQRAADEAGRLFPLSLAAEGSCQIGGNLSTNAGGVNVLRYGNAR
                     EQVLGLEVVLPDGQVWDGLRGLRKDNTGYDLKQLFLGAEGTLGVITAAVLRLYPKPAA
                     GATAWIALDSAHEAVELLAALRERLGERISAFELVSRTCLEAVLSHVAGTRDPLGAPH
                     PWYVLAQFADTEDLQTRVEQALADREAVIAQSVEQARSLWRIRESIPEAQFANVKHDI
                     SMPISKIPEFIGSAGGALAATFPGVRIFCFGHVGDGNLHYNVGSQALLEKRDAVNRIV
                     YDTVERLGGSISAEHGLGQAKREAIRRHKQPLELELMRRLKEALDPKGLMNPGKVL"
     gene            complement(13559..14299)
                     /locus_tag="VIG70_14225"
     CDS             complement(13559..14299)
                     /locus_tag="VIG70_14225"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8251755.1"
                     /translation="MRFGVALAATLAAAVVQANEVESRPPFITTPPEVVERMLEMAAT
                     RPEDLVIDLGSGDGRIVIAAAQKFGARGLGIELDAALVQKSREHARAANVAERASFVQ
                     GDVLVADISRATVVTVYLLPGLMGKLQSRFINELAPGTRIVSHEFTMAGWLPDRTETV
                     KLTAPHRGQGNQSRLHLWVVPADVRGVWRGPDMRLRIEQSYQRIEVEGARAATLSGRD
                     ISWDSFKGRVEGNRIVGELGGRPIELRR"
     gene            complement(14296..14790)
                     /locus_tag="VIG70_14230"
     CDS             complement(14296..14790)
                     /locus_tag="VIG70_14230"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8251756.1"
                     /translation="MRSIAGFLVFALLATPALAQVLKPWSGGATPPLALSDLEGTKHR
                     LADYRGKVVLVNFWATWCVPCRDEMPSIERLRASLEGKPFVVLAVNLAEPESRIRKFL
                     EAVPVRFPVLLDRDTQASKAWQARLLPVTYIVGPDGAVRYRYVGELDWSGPEARKAIL
                     GLLK"
     gene            complement(14857..15096)
                     /locus_tag="VIG70_14235"
     CDS             complement(14857..15096)
                     /locus_tag="VIG70_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251757.1"
                     /translation="MKKLLVALVASAFALGAYAQAPAPKGETKGTMKSETTKSDKSKA
                     KAKSGEAPKKAAKKDASKNAKKDAKKEKSNGGSGK"
     gene            complement(15183..16244)
                     /locus_tag="VIG70_14240"
     CDS             complement(15183..16244)
                     /locus_tag="VIG70_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEY8251758.1"
                     /translation="MSSRRPPIAGRFTAEEVALAARNRGMPLEALRHDVTPPGLHYLL
                     IHFDIPDADESRWQLEVGGLVDRPRSFSLAEIRALPAKTLRVTMECAGNGRGQLSPRY
                     PSVPWLEEGVSTADWTGVPLRELLELRAGAREVVFHGADRGIDVGVEHHFARSLARTQ
                     ALDSDVLVAYAMNGAPLPPQHGAPLRLIVPRWYGMASVKWLRSIEAREQPFDGVQQAR
                     GYHFRRAPGEKGEPCTLMRVNSLMAPPGIPDFYTRRRTVDAGPVEIIGRAWSGAAAVE
                     RVEFGLDGRWSPAVVAAERHAHGWTRWRFAWQAAPGEHELACRATDAAGNTQPLEPPW
                     DLSGFGNNGVQRIHVTVRP"
     gene            16270..16959
                     /locus_tag="VIG70_14245"
     CDS             16270..16959
                     /locus_tag="VIG70_14245"
                     /inference="COORDINATES: protein motif:HMM:NF016627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEY8251759.1"
                     /translation="MNLGQFTARLPPGDLWVFGYGSLMWSPCFDYKSKTLGRAHGYHR
                     ALCILSTRYRGTQRKPGLVMGLCRGGSCWGVAYRIDAPRLRRALARLWYREMPRRVYE
                     PRLIPVRIKTKTVRALAFVADPRHPSFVRELDLHGRARLVAQGIGIRGPCVDYIRNTL
                     DHMHDVGVRDPHLERILHAALALRQNGANDSRSVAGAPPPPAARQPEARAAGAVLRRA
                     ARRPRRAAARR"
     gene            17078..17650
                     /locus_tag="VIG70_14250"
     CDS             17078..17650
                     /locus_tag="VIG70_14250"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8251760.1"
                     /translation="MKKAEDFSSPLLAQGAFALTDPDGRRIVFGLPRNENAENGPVAR
                     LQHFVCATQRLPEMLEFYRNLGMVESDRVLEGEALSAVFLRSDPEHHSFAAFRAPESR
                     PDHHCYETTGWLDIRDWADRMGRLRIPLWWGPGRHGPGNNLFFMVEDPDGHKVEFSAE
                     LELMPRDMAHRTWPHEQRTLNLWGSAWMRS"
     gene            complement(17616..18737)
                     /locus_tag="VIG70_14255"
     CDS             complement(17616..18737)
                     /locus_tag="VIG70_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HEY8251761.1"
                     /translation="MKFDFDTPVDRSGTWSTRWERHAGRDIIPLWVADTDFRAAPAIL
                     EALLRRIEHGVFGYSTPPPELRAAIVERMQRLYRWRIDPSWIVFLTGVVPGLHLAARK
                     LVPPGGHAMIPRPVYHHFKRAMELAPRAFSETPLVLERGRWVFDLDALKQSKATTFFL
                     CNPQNPGGTVLRRAELERIADVTRDQVIVSDEIHCDLILEKALQHVPIASLAPEISRR
                     TVTLMSANKTFNIPAAGCAWAIIEDPGLRAAFSGDLAAHVVGSPSVFGLAATLAALTQ
                     CEDWLSAQLDYLRGNRDLVEKTIGFPMAHVEATYLAWVDCSSLGDATELFLKNGVAVY
                     PGTQFGEPGFIRLNFGTQRSRLKEALERIKSASTPSPRG"
     gene            complement(18801..20297)
                     /locus_tag="VIG70_14260"
     CDS             complement(18801..20297)
                     /locus_tag="VIG70_14260"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY8251762.1"
                     /translation="MSGLAALGMVMLLVMLGAIFIGFPISFTLLFLALTFGYFGLGHV
                     VFDLAYFQTIGMMKEELLAAVPLFIFMGFLTEQAGLMERLFHAFRMLLAPIRGALFAV
                     VILTATVFAMATGIVGAAVTVLGIMASPIMIKTGYDAKLAAGCITAGGTLGILIPPSV
                     MLIVMGPVIGVSVAELYSAAFGPGFLLAGIYLAYLVIRSNLNPKLGPPVPKEDRVSSP
                     VQLVTEVMVGVVPLLVLIGAVLGSMLAGLATPTEASAVGAAGASLLTLAYGKFTWAGL
                     KRAVLSTTATSSMVLLLAATSNVFGAVFARLGTANWITESLLGLPIPPVMMLIVVLVL
                     IFLLGWPFEWPAVILVFLPIFYPVMDGLKVTLAPALGIAPDMLMVWFGATVAVVLQTA
                     YLSPPVAMSAYYLKQVVKEWSLGTIYKGMFEFMLLQCICVVLIVAFPKIVTTFPEQLR
                     AEGALIKTEEVDDSANRLEEDPLKRAEEEAGQQEEESLEKDELSTKKK"
     gene            complement(20311..20862)
                     /locus_tag="VIG70_14265"
     CDS             complement(20311..20862)
                     /locus_tag="VIG70_14265"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY8251763.1"
                     /translation="MAQPAPGLIKTVRVIDKFTDVSGTWVAWLNVPLVLAVAYEVFAR
                     YAFNAPTIWSFDVTYMLYGTIFMLGAAYALLKGAHIRTDFFFERWSIRTKGIIDSIAY
                     LVFFFPAIVTFLVVSWNEGWYAFQIGETSEQTPWRPILWPFKMVVPLACLLLLIQGVS
                     ETIKSVWAVRTGIELDHKEKVEV"
     gene            complement(20933..22063)
                     /locus_tag="VIG70_14270"
     CDS             complement(20933..22063)
                     /locus_tag="VIG70_14270"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8251764.1"
                     /translation="MRHFVRSAVALAAVAAFSAGAQAQTTLKMQATWPASLTLYENFT
                     YFADRISKVSGGQLKIETMPAGQVVPAFEVLDATHKKVLDGAHAWSGYWVGKNKTAIL
                     CTGGPGGTFGMDMIDALGWMHHGGGNELCNELFQKELKLNVQWIPILPSGPQAFGWFK
                     RPIKNLADFKGMKCRQTGMAAEIWQRMGMTTVNMPGGEIIPSAQRGVIDCAEWVGGVE
                     DLRLGFHNVWKYHYTPGMHENVTIGEVVINGDVWKALKPEHQEMIKSIASETFVIWWA
                     KWQRQNADALKELQEKHGVRILRTPGDILVEFLKTWDKVAAEESAKNPFFKKVHESQR
                     QYASLVVPAKRFMFPPYSFAANYYFPESAAKSAAAKTDAKKK"
     gene            complement(22184..23317)
                     /locus_tag="VIG70_14275"
     CDS             complement(22184..23317)
                     /locus_tag="VIG70_14275"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8251765.1"
                     /translation="MKKALSLTALAAAALALNAQAQAPAAKQLKMQSTWPASLTLQDN
                     FRYFAERMDKLTSGGVKIEAMAAGQIVPAFEILDATHKRVIDGGHGVAYYWVGKNKAA
                     TLFSATPAGPFGMDHFDFLGWLQEGGGQALYDEFYRDVLKLNVVGMPIGGSSPQAMGW
                     FKRPIKDLADFKGMKCRQTGIVAEIYQRMGMQTVNMPGGEIVPSAQRGVIDCAEWVGG
                     VEDLRLGLPQVWKYHYTPGMHESNSMLEVIVNTDVWKGFTPQQQEAWRSAQMETFVRW
                     GVRWQKQNADAIEEMRTKHGTQMLRTPPAILIDFLKTWDKIAAEESEKSPFFKKVLDS
                     QREYASKVVPAKRFMFPPYSFAANYYFPEKKPAPEAKAKAKGK"
     gene            23353..24552
                     /locus_tag="VIG70_14280"
     CDS             23353..24552
                     /locus_tag="VIG70_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY8251766.1"
                     /translation="MKINRLETLRLGEFPNLVWLRVHTDQGLAGLGETSYAAQSVEAY
                     LHEYVAPRVLGRDPLEIEALGRGLTGYLGFRGAGVETRAASAFDLALWDLYGKVCNQP
                     LYQVLGGASRQRVRTYNTCAGYRYIRAAGGQSTKNWGLDGKPQGPYEDLDAFLHRADE
                     LAHSLLEQGITGMKIWPFDPHAETSDGQYISGPDLQKALEPFRKIRAAVGNRMDIMVE
                     FHSLWRLPAAQKIAQALAEFDTFWHEDPIRMDSLALLKQYAPYSKAPICASEILAGRW
                     SFKDLLETGVAGIVMFDLAWCGGITEARRIAALADAWQLPVAPHDCTGPLVYAASVHF
                     SIWAPNALIQESVRAFYTGWYNELVTHVPQPVQGYFGKPPGPGIGVELLPELWKRKDA
                     SVRVSPA"
     gene            complement(24539..>24940)
                     /locus_tag="VIG70_14285"
     CDS             complement(24539..>24940)
                     /locus_tag="VIG70_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEY8251767.1"
                     /translation="ERHRYAYEVEEVVGMVRRAYAGRVRFHNGDGEVAPGVTVHLIGG
                     HTMGLQVVRVKTRRGWVVLASDASHFYANMEDVRPFPIVYSVADMVEGYRKLLGLADS
                     PAHLIPGHDPLVLERYPSPTKELKGIAVRLD"
BASE COUNT         4210 a         8541 c         8295 g         3894 t
ORIGIN      
        1 ccaatccgac ggtggcggtg ctcgaggagc gcgtcgcggc gatcgagaac gggcgcgcgg
       61 cgctcgcctg cgccaccggc caggccgccg aggcgaccgc gatcctcacg ctgtgcagcg
      121 ccggcgatca catcgtctcc gcctccacgc tctacggcgg cacgcacacg ctgctgcacg
      181 tgaatttgaa gaagctgggc atcgagacga cgttcgtcaa cccggacgag ccggagaact
      241 tcaggaaagc gatccagaaa aacaccaagc tgctatacgc cgaaaccctg ggcaatccgc
      301 tcatcaacat cgtcgacatc gaggcgctgg cgaagatcgc gcacgaagcg gagatcccgc
      361 tagtgctcga caacaccgtg ccctcgccgt acctgtgccg gccgctcgag tggggcgcgg
      421 acatcgtggt gcactcggcg accaagtaca tcggcggcca cggcaccacg atgggcggcg
      481 tgatcgtcga gtcgggcaag ttcaactggg gcaacggcaa gttccccgag ttcaccagcc
      541 cctcgcccgg ctaccacggg gtgatcttcc acgagacctt cggcgacttc ggctacacca
      601 tgaaggcgcg catggagatc atgcgcacct tcggcccggc gctcgcgccg ctcaacgcct
      661 ggctgctgct gcagggcctg gagacgctgc acgtgcgcat ggagcgccat tgcgcgaacg
      721 ccctcgccgt ggcccggttt ctccagggcc atccgcgcgt cgcctgggtc aactaccccg
      781 ggcttcctga caacaaatat cacgccctag ccaagaaata cctgcccaag ggggcttcgg
      841 gcctgctcaa cttcggtgtg agaggagggg ccaaggcggg ggagcggttc atcgaggcgg
      901 cgcagttcat gagccacctc gcgaacatcg gagatgcgaa gacgctggtg atccatcccg
      961 cttcgaccac gcaccgtcag atggaggaag ccgatcagct gaaagcgggc gtcacgccgg
     1021 acatgatccg catctcggtc ggcatcgaga cgctcgacga cctcctttgg gacctggatc
     1081 aggcactgac tgaagcgggg aaagcgtgat cagctaccag atcatcgagc acggcaagcc
     1141 gctgcagcgg gttgctcttg agactccgaa accccagggg acggaagtgc tggtgcggat
     1201 cacgcgcgcc ggcgtgtgcc actccgacgt gcacatctgg gacggctact tcgacctcgg
     1261 cggcggcaag cgcttctacg tgaaggagcg cggctgcatc ccgcctttca cgctgggaca
     1321 tgagcccttt ggggtgatcg aagcactagg cccccgggct cgcggtgtgc gggtagggca
     1381 aaagaaaatc gtgtacccgt ggatcgggtg cggcaagtgc gcggtctgca aggcggggca
     1441 ggacaactat tgcgtctccg gcatccgctt cctcggcgtc agccggcccg gcgcgtattc
     1501 cacccacgtg ctggttccgg atcccaagta cctggtggat gccgccggca tcgaagaggg
     1561 cttcgccgcg acgctcgcct gctccggcct caccgcctac agcgccgcgg caaagctgcc
     1621 gccgatcggc gcgcaggatc gcgtcgcggt gctcggctgc ggcgggctcg gcttgatcgg
     1681 catctcggtg ctgcgcgcga aaggcgtgaa gcacatcgtc gcctgcgaca tcgatgacgc
     1741 gaagcttgcc gccgcaagca agcaaggcgc aaagacgact ctgaacacga aagcgcaaga
     1801 tgcgatgcaa cagctgcaag gcatcgccgc ggcgatcgat ttcgtcggca gcccggcgac
     1861 cgcggcgctc ggcatcggcg ccttgcggaa aggcggccgc tacgtgatct gcgggctgta
     1921 cggcggcgag ctcgtccatc cgctgccgcc gatcgcgcag cgcgccatcg gcatcgtcgg
     1981 ctcgtacgtc ggcaacctgc aggagctcaa ggaggtcgtg gcgctcgcga agaagcgcaa
     2041 gatcaaggcc gcgccggtcg agacgcgagc ggccgaagcc gcgagcgcgg cgctcgagga
     2101 tctcaaggca ggccgcgttc tcggacgcgt ggtgctcgac ttcgagagcg tgacggcatg
     2161 agcttcaggt ccaagggctc gccggacatc acgtcgcaga tggcgcgcga ccagctcgcg
     2221 gcgaaggcga aagccgtctc cgcgcagcgc tttgtctggc cggctccgcc gtactacgaa
     2281 ttctccgatc cttcccggca cagcgacgag tgcatgctgg tgttccggga cggcgccaag
     2341 acctcgggca cgatgctcga cttcaggccc gacgaggcgc agctcaagtt ccgcagccat
     2401 gattcgggcg acgacctcaa cgtcgcgttc tcctcgctgc ttcgggtgca gctgctgcag
     2461 ccggtgacgc tgcggcgcca gccgatgccg atcgacgaca gcgacgcgct gttcgccgcg
     2521 tccgagcgcc agccgttcgc gatcggcctg gcgaacggca agaccttcct gggggaaacg
     2581 gtcggcgcgg tccacgcgct gtgcggcctg ttcctgtttc cgccggagca ggaggggtat
     2641 gtcacgcgct ggttcgtgcc ggtgggcgcg acgcgctcgg cgagcatcgg caagccgctg
     2701 ggcgaaatgc tggtcgacga gaacctcgcc tccgccgagg cggtcgacgc ggcgctctcc
     2761 aagcagcagg cgatgcgcgc gcggcgcttc ggcgactacc tcaccgagca ccagatcgtc
     2821 tcgccggagc agctgagcac ggcgctcaag cagcagcgcg cccagccgat gcagaagatc
     2881 ggcgagaccc tggtggatct cggctacctc accgaagacg agctccagga agcgctcgcc
     2941 atcgaggcgc gcgaccgcag cgtgccgctc ggccagatcc tcgccgacat gggcgtggtc
     3001 gacgccgagg tcgtgaacgc gctgatggcg aggaagctcg gggtcccctt cgtcaacctg
     3061 aagaacttcc acatcgcgcc cgaagtgctg aagcgcgtgc cggccgcggt cgcgcaccgt
     3121 ttccagaccc tgccggtcgc cgaagccgaa acctcgctgg tggtcgcggt cgaaaacccg
     3181 accgacatgg cgaagctcga gggattgcgc ttcgtcgccg gcatgaagct cctgccggtc
     3241 atggcgtcgg gcgacgacat ccgcgcggcg ctcgaacgct gctacggccc gacacaagcg
     3301 ccgctttcct ccgtgcgcaa gacccccgat ctcgccatcg gcgagctcac ccaccgcctc
     3361 tcggtcgaga ccgccgacgc cgacctggac gagacccaat ccgccgccca ggacagcgcg
     3421 ctggtccagc tggtcaacaa gatgatcgtc gacgcgatcg agcagcaggc ttccgacatc
     3481 cacgtcgaga ccaacgcggc gggccgcaac ctgcgcgtgc gcttccgcaa ggacggcgtg
     3541 ctcgtgccct atctcgacct gccgtcgaag ttccgcaagg cgctcgtgtc gcgcatcaag
     3601 atcatgtgcc atctcgacat caccgagcgg cgcaagcccc aggacggcaa gatcgagttc
     3661 cggcgcttcg ggccgctcga cgtcgacctg cgcgtggcca ccattcccac cgccggcggc
     3721 ctggaagacg tggtgatgcg cgtcctcgcc gcggcgacgc cggtgccgct cgccgagctc
     3781 ggcttcgacc cgcagacgct cgagcgcatc aggcacctca tcacccggcc gcacggcctg
     3841 ttcctcgcgt gcggcccgac cggctccggc aagacgacga ccttgcattc cctgctcggc
     3901 tcgatcaaca ccgcggaaac gaaaatctgg accgccgagg atccgatcga gatcgcgcag
     3961 ccgggcctgc gccaggtgca ggtgaatccc aagatcggct ggaccttcgc cgcggcgatg
     4021 cgcagcttca tgcgcgccga tcccgacatc atcatggtgg gcgagatgcg cgacgccgag
     4081 accgccaaga tggggatcga ggcctcgctg accggccacc tcgtgctctc caccctgcac
     4141 accaacagcg cgccggaaag cgtgatccgg ctgctcgacc tcggcatgga tccgttcaat
     4201 ttcgcagacg ccctgctcgg cgtgctggcg cagcgcctgg tgcgcaggct gtgcccggat
     4261 tgcaagaccg gatatgcggc gagcaccccg gaactcgagg agctcgccac cgagtactgt
     4321 ggcgatcacg gcgacccggg caaggtcctg cagggctggc gcagccagcc ccggggaatc
     4381 gtgctttatc gcgccaaggg ctgcgcgaag tgcgaccgca ccggctacaa aggccgcatg
     4441 gggatctacg agctgctggt cgcgaacgcg gcgatcaagc gccaggtgca gacccgcgcc
     4501 ccggtgcagg agatcgccgc cgccgccacg gcgaacggca tgcggaccct gaagcaggac
     4561 ggcatcgaca aggtcatccg ggggctcacc gacctgcagc aggtccgtac cgtctgatct
     4621 cttagaatag gcctggtccg catgaaccag gcgcccaccg catgaagatg gaacacccgc
     4681 ccacgttttc gtgggccaac ccctatgcct ggccgcgcaa gccgctgctc gcagcgaacg
     4741 tggtttcgac ctcgcagccg ctcgccgcgc aggccggcct gcagatgctc gctgccggcg
     4801 gcagcgccgt cgatgcgatc ctcgccaccg cgatcaccct gaccctggtc gagccggtgt
     4861 cgaacggcat cggctcggat gcctatgcga tcgtctggga cggcagccgg ctccacggac
     4921 tcaacgcctc cggccgctcg cccgcggcgt ggagccccga atattttgcc ggccagaagg
     4981 cgatgccggt gcgaggctgg aactcggtga gcgtgcccgg ctgcgtctcg gcctggatgg
     5041 agatgcacaa gcgctggggc aagctgccgt tcgggaagct gttcgagcga gcgattcaat
     5101 acggccgcga gggtttcctc gtctcaccga cgatcgccgg ccaatgggaa aagcaagttc
     5161 ccgaactcaa gaatcaaccc ggctttgccg aggctttcct tccggacggt cgccctccca
     5221 gacccggcca acgcttcagg tttccggcgc acgccaacgt cctggaaaaa atcgcatcca
     5281 ctcgtggtga ggccttctat cgcggggaat tcgcggaaag gctcgaagcg cacgcgaaga
     5341 aacacgggag cgcgatgcgc gccgccgaca tggccgcgca caagtccgac tgggtcgaca
     5401 ccctgacgat ggactaccgc ggctatacgc tgcacgagat cccgcccaac ggccagggta
     5461 tcgtcgcttt gatggcgctc ggcatgctcg agcacttcga cctgcgctcg cacccggtcg
     5521 acgggcccga cagcgtgcac ctgcagatcg aggcgcagaa gctcgccttc gccgacgcct
     5581 ggcgctatgt cgccgacgtc gaccacatga aggacgttcg cccctcgtcg ctcctggaca
     5641 agagctatct gaaggaaaga gccaggctga tcgatccgaa gcgcgcgcag gatttcggcc
     5701 cgggcaagcc gcccaggggc ggcacggtgt acctcgccgc cgccgacgcc tcgggcatga
     5761 tggtgtcctt catccagtcg aactacatgg gcttcggctc gggcgtggtg gtcgacggca
     5821 tctcgctgca gaaccgcgcc gcgaccttca cgctgcaggc cggccacccg aactgcgtgg
     5881 gcccgggcaa gcgtccctac cagaccatca tccccggctt cgtcacgcgc gaaggcgcgc
     5941 ccgtgatgag cttcggcgtg atgggcggca ccatgcagcc gcagggccac gcccaggtga
     6001 tggtgcgcat cgccgactac ggccagagtc cgcaggcggc gtgcgacggc gcgcgcttcc
     6061 gcttcgtgca gaacatggac gtgagcgtcg aggacgggcg cttcccgccg gcaacgctgg
     6121 aagggctgca gcgccggggc caccgcatcg tcacgatcga cgactacaac cagttcggca
     6181 gcgcgcagct catctggaag ctcgatggcg gctatttcgt tgcgagtgat ccccggcggg
     6241 acggtcaggc agtcggcttc taaaacgtgg tctgtccctg attttcgctt cttgaggtgt
     6301 ctatgttgaa gatctggggc cgggtgaact cggtcaacgt caagaaggcc ctgtgggtga
     6361 tggaggagct cgggctcaag tacgagcgca tcgacgccgg catggagcac ggcgtggtca
     6421 agaccccgca gtacatgaag atgaacccga actcgctcgt gccgacgatc gacgacgacg
     6481 gcttcgtgct gtgggaatcg catgcgatcg ttcgctacct cgcagccaag cacggcgccg
     6541 gcacgctctg gccggcggac ctcaggcagc gcgccgactc cgaccgctgg atggactggg
     6601 cctacacctt ccaggcgcag ttccgcacgg tgttctgggg gctggtgcgc acgccgcccg
     6661 agaagcgcga ccttaaagcg attgaggaag cgcgcaagaa atgcggcgag ctgctcgcga
     6721 tccccgacca ggcgctttcg cacaagccct atttcgccgg caacagcctc accatcggcg
     6781 acatcccgct cggctgccac gtgcacctgt ggatgcggct gccgatcgag cgccccgcgc
     6841 acccgaacct gcagcgctgg ttcgaccggc tatgcgcgcg ggccgcgttc aagaaggtcg
     6901 tggatatccc tctttcctga agtaggcgtc gcgctcgggc tgcttggccg ccgccagctt
     6961 cttcacccgg cggtagaagt cctcgaggtc tccgctctcg gcgagcaggc gctcgaatgc
     7021 cggcacgagc tcggtgtaca ccgcgatcga aaggagaaac gcgttgttcg gttcggcggg
     7081 gagcgccgga taggaagcgc gcagccggtc ccattcgccg cgcttgtgct cgagcatcgc
     7141 ctcgcgcgcc aggtccatgc ggtacacccc cgcgaggcgc tcgcgcgcct ggctgacgcg
     7201 ctcggcgagc tcgcgctggc gcgcttgggc agcatggaac cccgcgagct cgcccgcgcg
     7261 tttctcggat tccagccagc gcttgacgcc ctcctgttcc accgtcacgg cgaacgactc
     7321 gttgaaggtc gtatcgtcgc gcgcgtacac cagctggtgc gcgagctcgt ggaacacgag
     7381 gcgagcgagc tgcgcatcgg agtagcggat gaaggtggag agcagggggt cgtcgaacca
     7441 gcccagggtc gaataggccg gcacgccgcc cacgtacacg tcgtagccct gttcgcgcag
     7501 ccggacggca tgccggcgcg cgtcgtcttc cgaatagaag ccgcgatagc tcacgcaacc
     7561 ggtgaagggg aagcactcct tcttcggctc gaccgagaac tccggcgcgg cgaacacgtt
     7621 ccacaccacg taggagcggt cgagatcggc atacgtgagg tagctgttgt tgtcgggcag
     7681 cttgagactc ttcgaggcga actcgcggat gcggcgcgcg gtttcgaggc gctgtttcag
     7741 ctcgggcggc gtcgccgggt cggcgatcca gtccgacacc ggccgcgccc tcgccatcat
     7801 cttgaggtgg ccgccgatgg cttgcgtgta atagtcaaag gactggcatc cggcgatcag
     7861 cacgaaaagc aaaattccaa caaaccacct catgcgaatc gccctgaagc gaggaagcgc
     7921 tcgaccaggc cgccgacctc tccggagacg atgagcatgc tgtggccctg gcgcaccagc
     7981 gcgcggccgg tcatgccgtc gacgctggtt tcctccacgc agaccacgcc gtcgttcggg
     8041 cccggcagcg cgccgccgag agcgcgcccg agtccgagcg gcagcgtgcc ggcgacgacg
     8101 ccgagcggcg ctgcgcgcgt ccaacgcgcc gcacgctcct cccagagcgc acggcacgcg
     8161 cccatcatcc agcggccgag ccccgcgcgc ccgagacgac gcccggcgag acaaccgcgc
     8221 accggcgcgc cgagtagcac cgcggcatcg gccgggatct ccgggtggcg catcagcatg
     8281 tcgaggatca gcacgccgcc caggctgtgg ccgacgaaga gcgtcttgcc gggagcgaaa
     8341 tccgccagcg cttcgacgtt ggcgtcatgg ggactgcggc cgcggtagtc aaagacatgc
     8401 acctcgtagc cgcggcgctt cagccgcgag gagagcagtg ccatcgcgac cgcgggcatc
     8461 cacagccccg ccgcgaggac cacgcgggcc ttcattcacc cttgccatca cgcgcgaaaa
     8521 tcgcgctcgc ccgcacgatg ggcttttcgt tgctgatcag gtagcaattg gcgaacgcca
     8581 gccgcgaccc cacgcgctgg atttcgacct tcgattcgat ccagtcgccg ggcctggcgg
     8641 cgccggcgta gtcgaccgcg aggtgcgccg taacgaagct gccgtgcctt tgtcccccta
     8701 tttcgcccga cttggcgagc atggcgtagc cgagcgcgag gtcggcgagc gtcgcgagca
     8761 cgccgccgtg gcagatgccg cgcgagttgg cgtgcttatc ctcgatggcg aggccgatcg
     8821 acaccgtctc gccgtcgcgc cgctcgtaga gcgggccaac gagcgcgttg aacgggctca
     8881 ggcgctcgat tttccggaag ccttcaggaa tgggtggcat cgcgcgcgat tttagcggtc
     8941 aggagcaggc gcagaccggc ggcgagcgcc ggcacgccga aaatcgcgta agtggcgccg
     9001 tacgaccccg tcagaaccaa aacaagatgg aaaagaggcg gtgtaacaac cacgccgagg
     9061 aatgtaaaaa atgcagagcc gccggtggcg tcgctgatgc gatcgcgcgg cgcgcggcgc
     9121 gcgatctcgg caaggaacac gccgttccag cccacggcgg tggcgccgta ggccatggcg
     9181 aaagcgaaca gcgcccacaa cggccaggcg ggcgacgccg ccagcgcggt gatcgcggtg
     9241 aggcccatgc caatgccgag gaggccgagc gttgcgcgcc gggtcgcgat gcggtcggcg
     9301 aataggcccc aaccgatgcg cgccgcgacg ctggtgagct gcgacgccgc cattaccatt
     9361 cccgccagga caagagagag ggagaaactt tcgactaaga acgtgaccag gtaggcgagc
     9421 agtgttattt gcatgccgcc gtagatgaac gacaccagcg acagctcgag gagcgttcgg
     9481 tcgcgcacga tgagggcgac cggcgcgaat gccgagcgga tcgacgccgg cgccgccgga
     9541 ttgcggccgg cgtcatagcg gccgcggatc ggcgccagcg cggcggcgca gagtgcgcag
     9601 gcgatgccga tggcgagcgc ggcggcctgc cagccgagag cgagcaccat caggggaacc
     9661 gcggcgccgg cgatcgccgt gccgagcggc acgccggtct gcttgatcga gaacatcagg
     9721 gcgagcaggt gcggcggagc ggtgcgcgcc aggatcaccg agctcgccgg ggtcgccggc
     9781 ccgtagccca ggccgaggag gagcgcccct acgaggaccg cgggcagcga tccgctcgcg
     9841 gcgacagcaa gccccgcgag gcaaagcagc aggccggcct gcgagaccag gatcggcccg
     9901 aagcgcccga cccagccgcc ggccgtcacc gtgccgatca tcgagccgag atacaccacc
     9961 gtgacgaaat agccgaccgc cgcaggcgtc accccgagcg ccggcgccgc ggccggcgcc
    10021 atcaccgggg cgcaataaac ggcggtcgac accagcgtct ggatcgccag cgtcgcggcg
    10081 acgggaagaa agactttcag ttcttgcgaa ccctgtccag cagcgcctga gcgcggtatg
    10141 cgacggggta gtccaccatc tcgccatcga tctggacggc ggcctctccc ttcgcctcgg
    10201 cctgcttgaa cgcggcgacg atgcgctcgg cgcgcgcgag ctcctcgccg ctcggcaggt
    10261 aggccgcgtt caccggcgcg atctggtcgg ggtgcacgca cagccggccc tggaagccgc
    10321 cgcggcggct gcgatcgagc gagcgcgcga acccgccggc ttccttgaaa tggaaccacg
    10381 ggctgtcgat cggcccctcg agccccgcgg cgcgcgaggc aagcacgacg cgggcgcgca
    10441 cctcggccag ctcggtttct tccagaccga ccgtcaggcc aagctcatgg gcgtagtcgg
    10501 cggcgccgaa ggcgacccgc ttcacgcgcc aaggccccct gtatgggcga aggctgcgcg
    10561 cctgcaggac gcgatcgatt cgctgcacgc ccgccgcggt ctcgatctgc ggcaccaggt
    10621 cgatcgagcc ctcggcaatg ccgcggctgc gctcgaggtt ccagatcacc cagtcgatcg
    10681 cgtgcaggtc ggccgcgctt tcgaccttgg gaaggaccac gccctcgacc ccgcggtgca
    10741 ggacctcagt cagatcgtca aagcagaaaa cgctcgaggg cgcgttcacg cgcacgtagc
    10801 ctcgcgggcg ccgcgggcgc tcgagcgcgg cggcgacggc cttccgcgtc gccgcctttt
    10861 cggcgagcgc gaccgagtcc tcgagatcga cgatcacggc gtccgcgtcc agggtgagcg
    10921 ccttctcgac gcggcgcgcc acgttgccgg gcgcgaaaag aaacgaacgg ttcatttgat
    10981 caacgccctc tcgctccatt gcgggtacgg cagaagcggc gtgccggtct ccgggcgccc
    11041 tctgcggatc tgcaccagcg cgggcctgaa gccgatctgc gcgccgtttt ccggcgcgac
    11101 gcggtacttg ccgagcgctg tcggcagctc gagctcggcg agtgccgcgc gcagcctttc
    11161 ctggtcgaga ttgccggcgc tgcgcactgc cgcggcgagc accgtgcccg ccgtgtaacc
    11221 ctcggccgcg gcgagctgcg gcggcacggt ccaccggacg gtgaacgcct tcgtgaacgc
    11281 gcgcgcttcc gcgtcgccga cgcgcggatc gaaatcgacc accccgaggc tgaactccgc
    11341 gtcctggccg agcagcgcga tgaagcgcgg gtgcgaggcg gcgccggcga aaaacagctg
    11401 cggcgcgtag tccaggcgct tgaaggtctt gaccatctcg gcggcgtcgc gcacgtcgcc
    11461 gaaggcgatc cacgcctcgg ctttcgcggc gcgcgccttc gccacctgcg cggcgaagtc
    11521 gacggtgccg gggcgataga gctcgacttc gccgggcacg aagccgttca gagccgtgtt
    11581 gcgcgttgcc tcggccattt cgcgggatgc ggcatcgtcg cgcgccagga taaagagcct
    11641 gcgcagctcg gccgtctcgg ccacaggcag cacgccaagg ccgtacacgg agtaaggcac
    11701 cgcggtctgg aagacgaacc gccccggccg gctgtgcacc acggctgccg gcccggcgcc
    11761 attcaccatc acgcggcgag cacgctcggc ttcggcggca gccagcagcg tggcggccga
    11821 gccgtagggg ccgatcagca gatcgacctt ggggtcgcgc agcagctccg catagagctc
    11881 gcgcgcgcgc accgcttgtg agttgtcgtc gaccacgcgc agctcgacac ggcgcccgag
    11941 caggccgccg gattcgttca tctgctcgcg ccacagctgc aggcccttga ggtaatcggc
    12001 ggcgagctcg gcgtgcgcgc cggtctgcga gatcgccgcg ccgacggtaa tcgtcagatg
    12061 cgccggcgca ggccgctcct gcgcgagggc aacgcagagc ccggctagcc aaacgaagag
    12121 aagccgaata gtgttcaagg atgaccttgc aacggcgcct gggcgacatt gtaggcgcgg
    12181 cccacgtgct cacggcgccc gcggacacga aaccgtactt caccgactgg cgcaagcagt
    12241 actcggcgcc ggcggagtgc gtcgtccggc cggcgagcac cgaggaagtc gcgcgggtgg
    12301 tcgcgctgtg cgcgcgcgag agcgtcgcag tcgtgcccca gggcgggaat accggcctcg
    12361 tcggcggctc ggtgccgacc ggcgcgcggc gcgagatcgt gctcgccctc ggccgcatga
    12421 accgcatccg ggcgctcgat gcgctgaacg acacgatcac ggtggaagcg ggatgcgtgc
    12481 tggccgcggt gcagcgcgcg gccgacgagg cggggcgcct cttcccgctc tccctcgcgg
    12541 ccgaagggag ctgccagatc ggcggcaatc tctcgaccaa cgccgggggc gtcaacgtcc
    12601 tgcgctacgg caacgcgcgc gagcaggtgc tcggcctcga ggtggtgctg cccgacgggc
    12661 aggtgtggga cgggctgcgc ggtctccgca aggacaacac cgggtacgac ctgaagcagc
    12721 tcttcctcgg cgccgaaggc acgctcggcg tgatcacggc ggcggtgctg cggctctatc
    12781 cgaagccggc cgccggcgcg accgcctgga ttgcgctcga cagcgcccac gaagcggtcg
    12841 agctgctcgc cgcgctgcgc gagcgcctgg gcgagcgcat ttccgccttc gagctcgtct
    12901 cgcgtacttg cctcgaggca gtgctttccc acgtcgccgg cacgcgcgat ccgctgggtg
    12961 cgccgcatcc ctggtatgtc cttgcgcaat tcgccgacac tgaggacctg cagacgcgtg
    13021 tcgagcaggc gcttgcggac cgcgaagccg tgatcgccca aagcgtcgag caggcgcgca
    13081 gcctctggcg catccgcgaa agcatccccg aagcgcagtt cgcgaacgtc aagcacgaca
    13141 tctcgatgcc gatctcgaaa attcccgagt tcatcgggag cgccggaggc gcgctggccg
    13201 cgactttccc cggggtgcgg atcttctgct tcggtcatgt cggcgacggc aaccttcact
    13261 acaacgtcgg gtcgcaggcg ctgctcgaga agcgcgacgc ggtgaaccgg atcgtctacg
    13321 acaccgtcga gcggcttggc ggctcgatct ccgccgagca cggcctgggc caggccaagc
    13381 gcgaggcgat ccgcagacac aagcagccgc tcgagctgga gctcatgcgg cggctcaagg
    13441 aagcgctcga tccgaagggc ctgatgaatc cgggcaaagt cctctagacc cgaaaaatcg
    13501 gggacagacc acgtttttcg cagctccgcg cagggaaaac ggggtctgtc cctgattttc
    13561 agcggcgtag ttcgatcggc cggccgccaa gctcgccgac aatgcgattg ccctcgacac
    13621 ggcctttgaa actgtcccaa ctgatatcgc gtcccgagag cgtcgccgcg cgagcgccct
    13681 ccacctcgat cctctggtag ctctgctcga tgcgcagccg catatccggg ccgcgccaca
    13741 cgccgcgcac gtcggcggga acgacccaca ggtgaagccg gctctggttc ccctggccgc
    13801 ggtgcggcgc cgtaagtttc acggtctcgg tccggtcggg cagccacccc gccatggtga
    13861 actcgtgcga cacgatgcgt gtgcccggcg cgagctcgtt gatgaagcgc gactggagct
    13921 tccccatgag ccccggaagc aggtacacgg tgaccacggt cgcccgcgag atgtcggcga
    13981 ccagcacgtc gccctgcacg aagctcgctc tttccgccac gttggccgcg cgggcgtgct
    14041 cgcgcgattt ctgcaccagc gcggcgtcga gctcgatgcc caggccgcgc gcgccgaact
    14101 tctgcgccgc cgcgatcacg atgcgcccgt cgcccgagcc gaggtcgatc accagatcct
    14161 ccggccgcgt cgcggccatc tcgagcatgc gctcgaccac ctcgggcggc gtggtgatga
    14221 acggcggccg cgattcgacc tcgtttgcct gcaccacggc agcggcgaga gtcgccgcca
    14281 gcgccacgcc gaacctcact tgagcaggcc cagaatggcc ttgcgcgcct ccggccccga
    14341 ccagtcgagc tcgccgacgt agcggtagcg gaccgcgccg tccgggccga cgatgtaggt
    14401 caccgggaga agcctcgcct gccaggcctt cgacgcctgc gtatcgcggt cgagcagcac
    14461 cgggaagcgc accggcaccg cctcgaggaa cttgcggatg cgagattcgg gctcggcgag
    14521 attgaccgcc agcacgacga agggcttgcc ttcgagcgaa gcgcgcaagc gctcgatgct
    14581 cggcatctcg tcgcggcacg gcacgcacca agtggcccag aaattcacca gcaccacttt
    14641 gccgcggtag tccgccagcc tgtgctttgt cccctcgagg tcggaaagcg cgagcggcgg
    14701 cgtcgcgccg ccactccagg gcttgaggac ttgcgcaagc gccggcgtgg ccagcagcgc
    14761 aaacacgaga aaacccgcga tggatctcat gggaacgccg aagcgtatcc caaaaaaaag
    14821 cccggccgga gccgggctcg cattccgatg cgggtgttac ttgccgctgc cgccgttcga
    14881 cttctctttc ttggcgtcct tcttcgcgtt cttgctggcg tccttcttgg ccgccttctt
    14941 cggcgcctcg ccgctcttcg ccttggcttt cgacttgtcg gacttcgtgg tttcgctctt
    15001 catcgtgcct ttggtttcgc ccttgggcgc gggagcctgt gcgtaggcgc ccagagcgaa
    15061 ggcagacgcg accagtgcaa ctagcagctt cttcaagatg gaatctcctc tgattgacga
    15121 gttgatccgg tacggatctg cgcagagcaa cgcgcgagtg cacccggccg ttgacgcgaa
    15181 cgttaaggcc tgactgtgac gtggattcgc tgcacgccgt tgttgccgaa gccggagagg
    15241 tcccagggcg gctcgagggg ctgcgtgttg ccggcggcgt cggtggcgcg gcaggcgagc
    15301 tcgtgctctc cgggggccgc ctgccaggcg aatctccagc gcgtccagcc atgcgcgtgt
    15361 cgttccgccg cgacaacggc ggggctccag cggccgtcga gcccgaactc gacgcgctcg
    15421 accgccgccg cgcccgacca cgcgcggccg atgatctcga ccggcccggc atcgacggtg
    15481 cgccggcgcg tatagaagtc ggggattccc ggtggcgcca tcagcgagtt cacgcgcatc
    15541 agggtgcacg gctccccctt ctcgccgggt gcgcggcgga agtgatagcc gcgcgcctgc
    15601 tgcacaccgt cgaagggctg ctcgcgcgcc tcgatggagc gcagccactt tacgctcgcc
    15661 atgccgtacc agcgcggcac gatgagccgc agcggcgccc cgtgctgcgg cggcagcggc
    15721 gcgccattca tcgcgtaggc caccagcacg tcgctgtcca aagcctgcgt tcgggcaagg
    15781 cttctcgcga agtggtgctc gacgccgaca tcgatgccgc gatcggcgcc gtgaaacacg
    15841 acttcccgtg ccccggctcg cagctccagc agctcgcgca gcgggacgcc ggtccagtcc
    15901 gcggtggaga cgccttcctc gagccaaggc accgacgggt agcgcggcga gagctggccg
    15961 cggccgttgc ctgcgcattc catcgtcacg cggagcgttt tcgcgggcag cgcccttatc
    16021 tcggcaagag agaacgagcg aggcctgtcg accaagcctc cgacctcgag ctgccagcgg
    16081 ctttcgtccg catcagggat gtcgaagtga atgagcaggt agtgcaggcc gggcggcgta
    16141 acgtcgtggc gcagcgcctc gagcggcatg ccgcggttgc gcgccgcgag cgcgacttcc
    16201 tcggcggtaa agcggcccgc gatcggcggc cggcgtgaag acatgcgccg attctataat
    16261 ccccgatcga tgaacctcgg tcagttcacg gcgcgcttgc ctccgggcga tctttgggtg
    16321 ttcggctatg gctcgctcat gtggtcgccg tgcttcgact acaagagcaa gacgctcgga
    16381 cgcgcgcacg gctatcaccg cgcgctgtgc atcctctcga cgcgctaccg cggcacgcag
    16441 cgcaagcccg gcctggtgat ggggctttgc cgcggcggct cgtgctgggg cgtggcctac
    16501 cgcatcgacg cgccgcggct gcggcgggcg ctcgcccggc tctggtatcg ggagatgccc
    16561 cggcgcgtct atgagccccg cctcatcccg gtcaggatca agaccaagac cgtgcgcgcg
    16621 ctcgcgttcg tagccgatcc ccggcatccg tccttcgtgc gcgagctcga cctgcacggg
    16681 cgcgcgcgcc tggtggcgca gggaatcggc atccgtggcc catgcgtcga ctacatccgc
    16741 aatacgctcg accacatgca cgacgtgggc gtgcgcgatc cgcacctcga gcgcatcctt
    16801 catgcggcgc tcgctttgcg tcagaatggc gcaaatgatt cccgatctgt ggccggcgcg
    16861 cctccaccac ctgcggcgcg acagcccgaa gcccgagccg ctggcgcggt tctacggcga
    16921 gctgctcggc gaccgcgtcg agcggctgcc cgacggtagc tggctggtcg ccggccgcgg
    16981 gcggcgcctc gtcgtcggca acggcgcggc ggcgtcagtc ccctacttcg cgctgcagct
    17041 ccaggatgcc gcgcaccttg ccgcgtttcg ccgcgcggtg aaaaaagccg aggatttctc
    17101 ctcgccgctt ctcgcgcagg gcgccttcgc gctgaccgac ccggatggcc ggcggatcgt
    17161 tttcgggcta cctcgcaacg aaaacgcgga gaacggtccc gttgcccgcc tgcagcactt
    17221 cgtctgcgcg acgcagcggc tgcccgagat gctcgagttc taccggaacc tcgggatggt
    17281 cgaatcggac cgcgtcctgg agggcgaagc gctgagcgca gtattcctgc gctccgatcc
    17341 cgagcaccac agcttcgcgg cgttccgcgc gcccgaatcg cggccggatc accattgcta
    17401 cgagaccacc ggctggctcg acatccgcga ctgggccgac cgcatggggc gcctgcgcat
    17461 cccgctatgg tgggggccgg ggcgccacgg cccgggcaac aacctgttct tcatggtcga
    17521 ggaccccgac ggccacaagg tcgagttctc cgccgagctc gagctgatgc cgagggacat
    17581 ggcgcaccgc acctggcccc acgagcagcg cacgctcaac ctctggggct cggcgtggat
    17641 gcgctcttga ttcgttccag agcttctttc aatcgagaac gctgagtccc gaagttcagc
    17701 cggatgaagc ccggctcgcc gaattgcgtg ccgggataga cggcaacacc gttcttcagg
    17761 aagagctcgg tcgcatcgcc caggctcgag cagtcgaccc aggcaaggta cgtcgcctcg
    17821 acatgcgcca tgggaaagcc gatcgtcttt tccaccaggt cgcggttgcc gcgcaggtag
    17881 tcgagctgcg ctgaaagcca gtcttcgcac tgcgtgagcg cggccagtgt cgccgcgagg
    17941 ccgaacaccg agggcgagcc gaccacgtgc gccgcgaggt cgccggaaaa cgcggcacgc
    18001 aggccgggat cctcgatgat cgcccaggcg cagcccgcgg cggggatgtt gaaagtcttg
    18061 ttggccgaca tgagcgtcac cgtgcgccgc gagatctcgg gcgccaagct ggcgatcggt
    18121 acatgttgga gtgctttttc caaaatcaaa tcacagtgaa tctcgtcgga gacgatgacc
    18181 tggtcgcgcg tgacgtcggc gatgcgctcg agctcggcgc ggcgcaatac ggtaccgccg
    18241 ggattctgcg ggttgcacag gaagaacgtg gtcgccttcg attgcttcaa cgcatccagg
    18301 tcgaaaaccc atcgcccacg ctcaagaacc agcggggtct cgctgaacgc gcgcggcgcg
    18361 agctccatgg cgcgcttgaa gtggtggtac accgggcgcg ggatcatcgc gtggccgccg
    18421 ggcgggacca gcttgcgcgc cgcgaggtgc agtcccggta cgacgccggt caggaacacg
    18481 atccacgacg gatcgatgcg ccagcggtag aggcgctgca tgcgttcgac aattgcggcg
    18541 cgcagctcgg gcggcggcgt gctgtagccg aatacgccgt gctcgatccg ccgcaagagc
    18601 gcttccagga tcgccggcgc ggcgcggaaa tcagtgtcgg cgacccacag cgggatgatg
    18661 tcgcgcccgg catggcgctc ccagcgcgtg ctccaggtcc cactgcggtc gaccggcgtg
    18721 tcgaaatcga acttcacagg ccttggaaaa gaaaaagggg cggagcgatt atctccgacc
    18781 cccttcaaag gtgctcgacc ctacttcttc ttggtcgaca gttcgtcttt ttctaggctc
    18841 tcctcctcct gctgtccggc ttcctcctcc gctcgcttga gcgggtcctc ttccaggcgg
    18901 ttggcgctgt cgtcgacctc ctcggtcttg atcaacgcgc cctccgcacg aagctgttcc
    18961 gggaaggtgg tcacgatctt cgggaatgcg acgatcagca cgacgcaaat gcactgcagc
    19021 agcatgaact cgaacatgcc cttgtagatg gtaccgagcg accattcctt caccacctgc
    19081 ttgaggtaat acgcgctcat cgcgaccggc ggcgacaggt acgcggtctg gagcaccacc
    19141 gccaccgtgg cgccgaacca gaccatcagc atgtccggcg cgatgccgag cgccggcgcg
    19201 agcgtcacct tcagtccgtc catcaccgga tagaagatcg gcaggaacac gagaatgacc
    19261 gcgggccatt cgaacggcca gcccagcagg aagatcagga cgagcaccac gatcagcatc
    19321 atcaccggcg gaatcggcag cccgagcagg gattcggtga tccagttcgc ggtgcccagg
    19381 cgcgcgaata ccgcgccgaa cacgttcgag gtggcggcga gcagcagcac catgctcgaa
    19441 gtggcggtgg tgctgagcac cgcgcgtttc agccccgccc aggtgaattt gccgtaggca
    19501 agcgtgagaa gcgaggcgcc ggcggcgccg accgcgctcg cctcggtcgg cgtcgcgagc
    19561 cccgccagca tcgagccgag caccgcgccg atcagcacca gcagcggcac cacgccgacc
    19621 atcacctcgg tcacgagctg cacgggcgag ctgacccggt cctccttcgg caccggcggg
    19681 cccagcttag ggttgaggtt cgaccggatc acgaggtacg cgaggtagat gccggcaagc
    19741 aggaagccgg ggccgaacgc cgccgagtag agctcggcca ccgagacgcc gatcactggt
    19801 cccatcacga tcagcatcac cgagggcgga atcaggatgc cgagcgtgcc gccggcagtg
    19861 atgcagcccg ccgcgagctt ggcgtcgtag ccggtcttga tcatgatcgg gctcgccatg
    19921 atgccgagca ccgtcaccgc ggcgccgacg atgccggtcg ccatcgcgaa tactgtggcg
    19981 gtcaggatca ccaccgcgaa cagggccccg cggatcggcg cgagcagcat gcggaacgcg
    20041 tggaacagcc gttccatcag cccggcctgc tcggtgagga agcccatgaa gatgaagagg
    20101 ggcaccgccg cgagcagctc ctccttcatc atgccgatgg tctggaagta ggcgaggtcg
    20161 aacaccacgt gccccaggcc gaagtagccg aaggtgagcg cgaggaacag cagcgtgaac
    20221 gagatcggga agccgatgaa gatcgcgccc agcatcacca gcagcatcac catgccgagg
    20281 gcggcgagcc cgctcatagg atgccccttt tcatacctcg accttttcct tgtggtcgag
    20341 ctcgatgccg gtgcgcaccg cccacacgct cttgatggtc tcggagacgc cctggatcag
    20401 cagcagcagg caggcgagcg gcaccaccat cttgaacggc cacaggatcg ggcgccacgg
    20461 cgtctgctcg gaggtctcgc cgatctggaa cgcgtaccag ccttcgttcc agctcacgac
    20521 caggaaagtg acgatggcgg gaaagaagaa caccaggtag gcgatcgagt cgatgattcc
    20581 tttagtgcgg atcgaccagc gctcgaagaa gaagtcggtg cggatgtgcg cgcccttgag
    20641 cagggcgtag gcggcgccga gcatgaagat cgtgccgtag agcatgtagg tgacgtcgaa
    20701 cgaccagatg gtcggcgcgt tgaacgcgta gcgcgcaaag acttcgtacg cgaccgcgag
    20761 caccagcggc acgttgagcc acgccaccca ggtgccggac acgtcggtga acttgtcgat
    20821 gacgcggacc gtcttgatca ggcccggcgc gggttgggcc atgcttgcca acgctcccct
    20881 tggataacca aaaaaaaggg cgattttcat cgccctttct cggactgctt agctacttct
    20941 tcttggcgtc ggtcttcgcc gccgcggact ttgcggcgct ttccgggaag tagtagttcg
    21001 ccgcgaacga gtacggcggg aacatgaagc gcttcgccgg caccaccagc gacgcgtact
    21061 ggcgctgcga ctcgtgcacc ttcttgaaga acgggttctt ggcgctctcc tcggcggcga
    21121 ccttgtccca ggtcttcagg aactcgacca ggatgtcccc gggggtgcgc aggatgcgca
    21181 cgccgtgctt ctcctgcagc tccttcagcg cgtcggcgtt ctggcgctgc cacttcgccc
    21241 accagatgac gaaggtctcg ctcgctatcg acttgatcat ctcctggtgc tcgggcttga
    21301 gggccttcca gacgtcgccg ttgatcacca cctcgccgat cgtgacgttc tcgtgcatgc
    21361 ccggcgtgta gtggtacttc cagacgttgt ggaaaccgag gcgcaggtcc tcgacgccgc
    21421 cgacccactc ggcgcagtcg atcacgccgc gctgcgccga cgggatgatc tcgccgccgg
    21481 gcatgttaac cgtggtcatg cccatccgct gccagatctc tgcggccatg ccggtctggc
    21541 ggcacttcat gcctttgaag tcggcgaggt tcttgatcgg gcgcttgaac cagccgaaag
    21601 cctgcgggcc cgacggcagg atcgggatcc actgcacgtt gagcttgagc tccttctgga
    21661 acagctcgtt gcacagctcg ttgccgccgc cgtggtgcat ccagcccagc gcgtcgatca
    21721 tgtccatgcc gaaggtgccg ccggggccgc cggtgcacag gatcgcggtc ttgttcttgc
    21781 cgacccagta gcccgaccag gcgtgcgcgc cgtcgagcac tttcttgtgg gtggcgtcga
    21841 gcacttcgaa cgccgggacg acctgccccg cgggcatggt ctcgatcttg agctggccgc
    21901 cggaaacttt gctgatgcgg tcggcgaagt aggtgaagtt ctcgtacagc gtcaggctgg
    21961 cgggccaggt ggcctgcatc ttgagggtcg tctgagcctg cgcgccggcc gaaaatgccg
    22021 cgacggctgc aagcgccacc gcagaacgga cgaaatgcct cataagcttc ctcctcctcg
    22081 tataggggtt ccctaggcga aggcagggta cacgcttgtt tttgctcggt caacgaatga
    22141 acaggggacg ccgaagcgtc cccttgccgt gcgcttccag gggttactta cccttcgcct
    22201 tcgccttggc ttccggcgcc ggcttcttct ccgggaagta gtagttcgcc gcaaacgagt
    22261 acggcgggaa catgaagcgc ttcgccggca ccaccttgga cgcgtactcg cgctgcgagt
    22321 cgagcacctt cttgaagaac gggctcttct ccgactcctc ggcggcgatc ttgtcccagg
    22381 tcttcaggaa gtcgatcagg atcgcgggcg gcgtgcgcag catctgcgtg ccgtgcttag
    22441 tgcgcatctc ctcgatcgcg tcggcgttct gcttctgcca gcgcacgccc cagcgcacga
    22501 aggtttccat ctgcgccgag cgccacgcct cctgctgctg cggcgtgaag cccttccaca
    22561 cgtcggtgtt gacgatcacc tcgagcatcg agttcgactc gtgcatcccc ggcgtgtagt
    22621 ggtacttcca gacttggggc aaaccgaggc gcaggtcttc gacgccgccg acccactcgg
    22681 cgcagtcgat cacgccgcgc tgcgccgagg gcacgatctc gccgccgggc atgttcaccg
    22741 tctgcatgcc catgcgctgg tagatctcgg cgacgatgcc cgtctgccgg cacttcatgc
    22801 ccttgaagtc ggcgaggtcc ttgatcgggc gcttgaacca gcccatcgcc tgcgggctcg
    22861 agccgccgat cggcatgccg accacgttga gcttgaggac gtcgcggtag aactcgtcgt
    22921 agagcgcctg gccgccgccc tcctgcagcc agccgaggaa gtcgaagtgg tccatgccga
    22981 acgggccggc cggcgtggcg gagaacagcg tcgccgcctt gttcttgccc acccagtagt
    23041 acgcgacgcc gtggccgccg tcgatcaccc tcttgtgcgt ggcgtcgagg atctcgaacg
    23101 ccggcacgat ctggcccgcc gccatcgctt cgatcttcac gccgccgctg gtgagcttgt
    23161 ccatgcgctc ggcgaagtag cggaagttgt cctgcagcgt gaggctcgcc ggccaggtcg
    23221 actgcatctt aagctgcttc gccgccggcg cctgcgcctg tgcattgaga gcgagcgcgg
    23281 ccgcagcgag cgcggtaagc gacaatgctt ttttcatggg accctcctcc tgagagagag
    23341 cgggaatgtt atatgaagat caatcgactg gaaacgctgc ggctcgggga attccccaat
    23401 ctcgtctggc tgcgcgtgca caccgaccag gggctcgcgg gcctaggcga gaccagctac
    23461 gcggcgcaga gtgtcgaggc gtacctgcac gaatatgtgg cgccgcgcgt cctcggccgc
    23521 gatcccctcg agatcgaggc gctcggccgc ggcctcaccg gctacctcgg cttccgtggc
    23581 gcgggcgtcg agacgcgcgc ggcgtcggcg ttcgacctgg cgctctggga cctgtacggc
    23641 aaggtgtgca accagccgct ctaccaggtg ctcggcggcg cgagccggca gcgcgtgcgc
    23701 acctacaaca cgtgcgccgg ctaccgctat atccgcgccg ccggcgggca gagcacgaag
    23761 aactggggcc tcgacgggaa accgcagggg ccctatgagg acctggacgc cttcctgcat
    23821 cgcgccgacg agctcgcgca ctcgctcctc gagcagggga tcaccggcat gaagatctgg
    23881 cccttcgatc cgcacgccga gaccagcgac gggcagtaca tctcgggccc ggatctgcaa
    23941 aaagcgctcg agcccttccg aaaaatccgc gctgccgtgg gcaaccgcat ggacatcatg
    24001 gtcgagttcc actcgctgtg gcgcctgccc gcggcgcaga agatcgcgca ggcgctcgcc
    24061 gagttcgaca ccttctggca cgaagacccg atccggatgg acagcctcgc cctcctcaag
    24121 caatacgcgc cctactcgaa agcgccgatc tgcgcctccg agatcctcgc cgggcgctgg
    24181 agcttcaagg acctgctcga gaccggcgtc gcggggatcg tgatgttcga tctcgcctgg
    24241 tgcggcggga tcaccgaagc gcgccgcatc gccgcgctcg ccgacgcgtg gcaactaccg
    24301 gtcgcgccgc acgactgcac cgggccgctg gtgtacgcag cttcggtaca tttctcgatc
    24361 tgggcgccga acgcgctcat ccaggaatcg gtacgcgcct tctacaccgg ctggtacaac
    24421 gagctggtga cgcacgtgcc gcagcccgtg cagggctact tcggcaaacc gccgggtccc
    24481 ggcatcggcg tcgagctgct tcctgaactt tggaaacgaa aagacgcgtc ggtgcgagtc
    24541 agtccagcct gaccgcgatt cccttcagct ccttcgtcgg cgaggggtag cgctcgagca
    24601 ccagcgggtc gtggcccgga atgaggtgcg ccggcgagtc ggcgagcccg agcagcttgc
    24661 ggtagccctc gaccatgtcc gccaccgagt agacgatcgg aaacggacga acgtcttcca
    24721 tgttggcgta gaaatggctc gcgtcggagg cgagcaccac ccagccgcgc cgcgtcttca
    24781 cccgcaccac ctgcaggccc atggtgtgcc cgccgatcag gtgcacggtg acgcccggcg
    24841 cgacctcgcc gtcgccatta tggaagcgca cccgcccggc atacgcgcgc cgcaccatgc
    24901 cgaccacctc ctcgacctcg tacgcgtagc ggtggcgctc
//
LOCUS       DATBSZ010000229        24774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2297405, whole genome shotgun sequence.
ACCESSION   DATBSZ010000229 DATBSZ010000000
VERSION     DATBSZ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 24774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24774
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2297405"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VIG70_14290"
     CDS             complement(<1..235)
                     /locus_tag="VIG70_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY8251768.1"
                     /translation="MAVLVVAEHDNKTVKKATLNTVAAAQKLGSDIHVLIAGHQAGEA
                     AKAASAISGISKVLLADAPHLGEFLAENVAALLV"
     gene            complement(235..984)
                     /locus_tag="VIG70_14295"
     CDS             complement(235..984)
                     /locus_tag="VIG70_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY8251769.1"
                     /translation="MKVLVAVKRVIDFNVKVRVKADGTGVETANVKMSMNPFDEIAVE
                     EAVRLKEAGVATELVVVSCGAQSCQETLRTALAIGADRAILVETAAELQPLAVAKLLK
                     AVCQKESPKLVILGKQAIDDDSNQTGQMLAALLGWPQATFASKLKIEGEKAQVTREVD
                     GGLETVSIKIPAVVTTDLRLNEPRYVTLPNIMKAKKKTLEVLKPEALGVDVAPRLATV
                     KVVEPGKRKAGTKVADVKALVDKLRNEAKVI"
     gene            1013..1792
                     /locus_tag="VIG70_14300"
     CDS             1013..1792
                     /locus_tag="VIG70_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY8251770.1"
                     /translation="MSDLSQLNIKSMRERVSNEEWQARVDLAACYRLVHHYGMDDLVY
                     NHISARVPGEEGHFLINAYGMMYDEITASSLVKIDFDGNVVQDSGTGYGINQAGFVIH
                     SAVHKGRPDVGCVIHTHSIAGMAVSALDCGLLPITQTAMYFDGVGMHEYESVAIDLDE
                     QQRLVRDLGRFKAMILRNHGLLAVGPTVSEAFTNLFWLERACKAQVAAMACNAKLHLP
                     AQDVIEKTNHLYRPETRRRWGPLEWPALLRLADRKYPGFRD"
     gene            1789..2700
                     /locus_tag="VIG70_14305"
     CDS             1789..2700
                     /locus_tag="VIG70_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8251771.1"
                     /translation="MKPLQGRSVIVTGAGGGIGRDFALAMAAAGAKVLVNDLGTSVRG
                     EGRDAGPAQQVVNQIKEKGGIAVANTDSVAEWESANRIVSSAVEAFGRLDVVVNNAGI
                     LRDRFFFNMSVEEWRAVIDVHLNGSFYVSRAAAPHFKSQNSGCYVHMTSTSGLVGNLG
                     QANYSAAKLGIAGLSKSIALDMAKYNVRSNCISPFAWSRMIGSIPAETDDQKARVEKL
                     KSMETAKIAPLAVFLASEEAQDVTGQIFAVRANEIFLMSQSRPLRSVHRAQGWTPETI
                     ASHAIPSLRAHFYPLDRSQDVFSWDPS"
     gene            2697..3668
                     /locus_tag="VIG70_14310"
     CDS             2697..3668
                     /locus_tag="VIG70_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8251772.1"
                     /translation="MKPRVLLTDAIAPAGEALLARTVEVVRAPDSEPSTMRRCARDAD
                     GVIIRSKLPDDIFEAAPRLRGVVVHGTGTDLVPVAAATEHGIMVANLPGINAQSVAEY
                     CALAMLMLARNILSIVSSLKSEAWDAARVRGAGAHEIAGMTLGIVGVGEIGGRLAKIA
                     RHGFGMNVLGHQRRLDRVPAEASPVSLEELLAKSDFVVLACPLTPQTRYLMNARTIGL
                     MKPAAWLINVGRGPVVQEEALIEALRGKRIAGAMLDVYEHYRLEPGHLLFTLDNVILT
                     PHLAAVTQESRARAGVAAADEMLRILRGERPRNLVNPEVLKKEQLKT"
     gene            3665..4816
                     /locus_tag="VIG70_14315"
     CDS             3665..4816
                     /locus_tag="VIG70_14315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8251773.1"
                     /translation="MNLGRESHPELRDAVRQLCSQFDSAYWQKIDEKRAYPEAFVDAL
                     TKAGWMSALIPEEYGGSGLSLTEATVIMEEVTRSGGNAGCCHGQMYNMNTLLRHGSAQ
                     QKKKYLPKIASGELRLQSMGVTEPGAGTDTTKIKTVAKKKGDRYLVNGQKVWTSRLQH
                     SELMILLARTTPLTEVKRKSEGMSIFLVETKSKGLEVKPIRNMVNHETNSVFIDNLEI
                     PADNLIGEEGKGFRYILDGLNAERILIAAECIGDGYWFVEKSCSYSKDRVVFDRPIGQ
                     NQGIQFPIAEAYMEIEAANLMRYKAAMLFDSHKPCGAEANMAKHLAAKASWEAANVCM
                     QTYGGFGFAAEYDVERKFRENRLYLVAPISTNLILSYLAEHVLGLPRSF"
     gene            complement(4824..5930)
                     /locus_tag="VIG70_14320"
     CDS             complement(4824..5930)
                     /locus_tag="VIG70_14320"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251774.1"
                     /translation="MRIAAIATAVCLLASAQASGGEIMVAQVAAFTGAQASSGKAIRA
                     GINLYLNHVNRNGGIGGERILLATYDDGYKAEETVRLVKEVLAKEAPIAFIGVLGTAN
                     NEALIKDGVLARANVPLVGAISGASTMIGAPNVFVTKASYGDEVRRLFEILSLIGINR
                     VAVVYQDDGFGKDIIAGAERAAPATRVNLVVKAPYERNTTNVGAAVQAVLKSDAQVIY
                     LGTVTSAAVEFIKQYRQGGGSAQIYGVSVIDINAMKRSLSGDELSGYGFGVLWPLTTA
                     RSFRLIREYQQLAQAAKDPDLAERSMEGYIAAKVLVHGLRQARSSPAALAKAVRGMKS
                     VDLGGYFVDFTQPKQTGSQFVEFAILSKTGHILQ"
     gene            6140..6622
                     /locus_tag="VIG70_14325"
     CDS             6140..6622
                     /locus_tag="VIG70_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEY8251775.1"
                     /translation="MRYPQKGFTLIELMIIVGIIGILSSLVLPAYQDYSVRTKMSEVV
                     LALTSCRVSITEVYQIGPDTAPGPNGWGCEAGATPGTTYVSSVTTTADGVTSATVRGI
                     TTAVNGAVVTLAPLAPPAPGTPATFVPGTSQPLYGWRCGSAADGTTVAARYLPSSCRG
                     "
     gene            complement(6624..7922)
                     /locus_tag="VIG70_14330"
     CDS             complement(6624..7922)
                     /locus_tag="VIG70_14330"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8251776.1"
                     /translation="MKVEGPQAQQMIGKYPVIREIGSGATSRVYLAHDPFAEREVAIK
                     VLQFARRVDRDTERMVHKAFVAEASLAGKLNHPHIVDIYDAVVDPDRSYLVMEYVPGT
                     TLDSHATPTSLLPLSKVVEIIFKCIRALEYAFQHGIIHRDIKPGNVLLSELGETKVGD
                     FGASFQERLDDKTTQLKGVGSPAYMSPEQLRMEKLTQQTDIYSLGVTMFRLLTGRLPF
                     EAGSHVALTYAILHSPPPRPSSFRPDLPPLLDEIVMKAIARNPAERYRSWLEFGKAVT
                     RAFMSLRPTDATASDSEKFGKLRDMSFFKDFGEVELWEVVRVATWQTLEAGTVLMREN
                     DQGDSLYVLVDGEVEVSLLGKPLATLKPGGCFGEILYFADSVERRTTTVTARDRITVM
                     QIKAGALRVATAACQVGFNKAFMRVLVERLAQANRQLAQR"
     gene            complement(7919..12910)
                     /locus_tag="VIG70_14335"
     CDS             complement(7919..12910)
                     /locus_tag="VIG70_14335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY8251777.1"
                     /translation="MTDTTLLSWINAEVDRALTLVQESIAKFAVAPDNDAVLQPCPEH
                     LHQVSGALRMVGLSGATRVCEAIEGSFASMGASRPTPAAMDVIERAVLALKNFVGELA
                     RGHTDVPMRLFPVYRDVAGLVGKSAISEKDLFYPDLSQPAPPHTSMKAMPEDELPAFL
                     QAQRARFQRGLLAWLRGGPAGLEEMRQTTDALYQVATQLPGPRALWWAAGALIESLAV
                     EPASADPQWLAAAKVVCNRLDFQIRDLAAGAAKNGDALLREVLYSIAKREVVTARVKQ
                     VKQLYQLESLFPRAEKPAAVQELDAERLEASLFDMHSRLDALKRAWEQYVAGERDKAR
                     AFRERVADFKTKTKDLGNQQLIKLLDAIALVAGKLPEPYPRQEQMMVIEMASAFLLVE
                     HVIDHFSSPAEDLEAQIVIMGGWLLDAAKGKSTGEPPAGLRPDLTERIGALQLRAQVA
                     KEILANLQHSEQVIDAFARDATKRATLADLQPYLRQMHGALSVLGFPGAAQAVSICES
                     LILAQARGEGTGPGDMDWIAEGLSSVGFFLEPCRHGQDPQEEAIALFFRRYEKRHVQA
                     TIDSTLRLEFPSRTSPVAAAPARPKVEAAPRPKVDTGPRPKVDGELLQVFLEEAEEVL
                     QTIKTTLPECREKPEDREPQTVVRRAFHTLKGSGRMVGLMDLGEAAWQVEQVMNAWLE
                     QKRAVTPALLELLETAAGSFSEWIAKLRGPGLEGLIDDSKIVELAAWAKAAQAEAVAV
                     PTVTVGTVTVARNLYDIYTKEAAEHIAVLRAECAKWRETAPGPVSADFMRAAHTLGST
                     SHTAGFIPLAELAGSLEEWSVFAEQTTDAADAETVQAAVVDLGIMVEAISRGDAPAIS
                     QMPQDTLRELIGRLESARLAAHRAGTTLPEPVPAAAPVPAVAPPPAPAAAPADGREKR
                     RMLDDIDAQLLPIFLEEAQQLVPDIGSDLREWKANPSEAKISQSLRRSLHTLKGSARM
                     AGAIRLGELCHLMEGRIESALEAGGALPADLFEYLQERMDRLSTDVERMAAPAPAAPA
                     FSPSTTEVQPQPRAEAPLPTPAAMLRVNADTLDHLINEAGEIAIARTRAEAELRAIKQ
                     SLADLTESIARLRNQLREVEVQADSQMQSRLSVMDEKKGEFDPLEFDRYTRLQELTRL
                     MAESLNDVMSIQQGLHKYIGDTDAALLHQARTSREVQQDLMRVRAVPFSNLSERLYRI
                     VRQIAREIGKKAELEITGAEVELDRGVLERISAPLEHMLRNALVHGIEDPAARAAAGK
                     AESGRIAIALRQESNEIALIFSDDGAGLNLEALRRRGAAMGLLPADREPTEGEIAQLV
                     FASGLSTAEAVTEVAGRGVGMDVVRNEISLIGGRVDILTAQGRGTTFTVYLPLTLAVT
                     QAVLVRSGENVIALATAMVEQVLRLKSDALALSYEKKSIDFQDRAYPLHAMQQLLGRA
                     SAGELHPYNSVLLLRSGVQRVAVHVDELLGNQEIVVKSIGAQLGRVPGITGATVLGDG
                     RIALIMNPVQLAQRQRAAPAAARSMFTTTSLLRDTRGSLRDSTSIRAARAPVVMVVDD
                     SLTVRKITGRLLEREGYRVLTAKDGVDALEKMAESLPRVMLVDIEMPRMDGFDLARHV
                     RADARTQGVPIIFISSRTAEKHRNQAAQIGVNAFLGKPYPEAELLQLVAKYTA"
     gene            complement(12943..14970)
                     /locus_tag="VIG70_14340"
     CDS             complement(12943..14970)
                     /locus_tag="VIG70_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8251778.1"
                     /translation="MALNLFGKLSTGRRLQILIGLLVALLAIDAVVVWFDARQATFGT
                     IYIATAGKIRMLSQRLAKAAQQASQGNLEAFKQLRESRDEFASQMNLLLAGGASAGVA
                     LPPTSSGVRPVLETLNAHWKKNERNAALVIVEQNNLLNLGKAVRAINARSPVLQELTD
                     EVAALSVQSGGSARQNALVSQLMMLTQRMAKNANTMLAEDVIDPEVSFLLGKDANSFR
                     DILQGLLAGSEALRIQRAADPELRGKLAEVEASFKEYQAGVSSILGNQQQLVNAKRAS
                     FELFKDSEELLGAAENLNTAYEAELVGRRLNFIVLAAVSLLALVVLLLIGKVYLDDSG
                     RRAEESERQNRENQQAIQRLLDEIGNLANGDLTVRAQVTEHMTGAIADSINYTIDELR
                     RLVAGITSASLQVTSATQEAETVSGKLLQAASKQAVEIQGAGQSVAQMAVSMSDVSKK
                     ANDSSKVAESSLHAAQKGAQAVQNAIRGMNDIRVQIQETSKRIKRLGESSQEIGEIVQ
                     LISDITEQTNVLALNAAIQAASAGEAGRGFTVVAEEVQRLAERSAEATKHIGAIVKSI
                     QRDTQEAIDAMERSTQGVVQGTKTADEADQALREIGTVSRQLAELIESISAATQQQAT
                     SAGKVASNMKVILGITQLTTDGTKQTATSAARLTALADGLKSSVAGFKLAA"
     gene            complement(15010..16131)
                     /locus_tag="VIG70_14345"
     CDS             complement(15010..16131)
                     /locus_tag="VIG70_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251779.1"
                     /translation="MRRILCILATWLPAIAAAEPGVTSERIFLGQAAVFTGPAAQLGI
                     QMRNGIKTYLDHVNAKGGVYGRKLELLTEDDRYEASVAPGASRKLIEEHRVFALLGYV
                     GTPTGVAHLPVVTQARVPLVGMFTGAEVLRAPFNRYVFHVRASYYDETEKIVEQVVST
                     GGRKIAVFYQNDAYGEAGRKGTELALERRKLKIHSQGTVERNTVQVEAAAKAIHASQP
                     DAVVMVSAYTSIAAFVRAMQKQGSAATFYNVSFVGSKALADALGQDGVGVAISQVVPF
                     PWGTSVPVVKEYQQLAKKSGYTDYNFSALEGFLSAKVMVEGLKRAGKNPTREGLVDSL
                     EKMQDVDLGGFFIGYSPTNRAGSRFVDLTIISRDGRFLR"
     gene            complement(16143..16667)
                     /locus_tag="VIG70_14350"
     CDS             complement(16143..16667)
                     /locus_tag="VIG70_14350"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY8251780.1"
                     /translation="MEQGITRARVSLREYQRELAERLRLADSARSASKLGVQVGEQNW
                     LIDLVEASEVLRVPPITPVPLTRPWFRGVANVRGNLYSVVDFAEFLGTGISGTGEDAR
                     LVLLGERFRSAAALLVDRSLGLRNPAHLRLREAAPGRAAWLRGEYEDEGGGRWSELNV
                     AELVRDAEFLTVAL"
     gene            complement(16667..17032)
                     /locus_tag="VIG70_14355"
     CDS             complement(16667..17032)
                     /locus_tag="VIG70_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8251781.1"
                     /translation="MPIKNILVVDDSATDRQYLTDLLAKNGYKVETAQNADEALAKAK
                     QMRPDLVLMDIVMPGQNGFQATRALSKDEATKTIPVIICTTKGQETDRAWGMKQGARD
                     YIVKPVKGPELLKKISALG"
     gene            complement(17052..17420)
                     /locus_tag="VIG70_14360"
     CDS             complement(17052..17420)
                     /locus_tag="VIG70_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8251782.1"
                     /translation="MKVLVIDDSNTIRRSAELFLRQAGYEVILAEDGFDALAKISDHQ
                     PQVIFVDIMMPRLDGYQTCALIKQHPQLKTTPVIMLSSKDGVFDRARGRLAGSDRYLT
                     KPFTKEGLVEAVTEHLKPSP"
     gene            complement(17658..18842)
                     /locus_tag="VIG70_14365"
     CDS             complement(17658..18842)
                     /locus_tag="VIG70_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8251783.1"
                     /translation="MKVVVAGGGPAGMMLGLLLARAGIETLVLEKHADFLRDFRGDTL
                     HPSTLEVLAELGLLERLLELPHDRVPEIAAQFGDYPLKIADFRHLPTRCGFIAMMPQW
                     DFLDFLAREAARYPQFQLRMNAEVTGLIESKGRVAGLRFSDEKAEADLVIAADGRNST
                     VRRSAGLEVETLGAPIDVLWFRLSRHPDDPQSSMGRFDTGAALVLINRRAYWQCGYVI
                     PKGAADSVRGAGLPAFRATLARLLPWVAPRVAEIADWEQVKLLTVQIDRLKQWWRPGL
                     LCIGDAAHAMSPVGGVGINLAIQDAVAAANILSEKDFSIQKLKKIQRRREWPVRVTQR
                     LQILAQDRIITRVLGSTGPLSPPLFFRWVNRFALLRRLPGRLIGMGVRPEHVATPHAA
                     SQ"
     gene            complement(18847..19812)
                     /locus_tag="VIG70_14370"
     CDS             complement(18847..19812)
                     /locus_tag="VIG70_14370"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY8251784.1"
                     /translation="MKRVALAVLGLAVALPAMPQGFTPTRPVEVVVHTGPGGGSDVLA
                     RAMVLMMEKEKLLPVRAQVANKPGGGGVVAAAYLAEKKGETHTIGIFTGVWLTNPLTS
                     NEAKVSLKDLTPIARLLLEPAVIAVKADAPYKTLGDFVSAAKQKPGELKQSGGSITSR
                     DNVVRQQLQKVSGARWQFISFPGGGERIAALLGGHVNMMVIEPNEAGEHIRAGNMRLL
                     AQVNDKRLAAYANVPTLKEAGFDVTPVPQVRGVVAPPGIPRENVAYWEGVFKKMSQTE
                     SWKKYVADNQFEDAYQNAAELTKFYDDFTGQMRGILQEAGVKTVR"
     gene            complement(19828..21333)
                     /locus_tag="VIG70_14375"
     CDS             complement(19828..21333)
                     /locus_tag="VIG70_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY8251785.1"
                     /translation="MDVLSGLMQGFAVALQPMNLLWCFLGVVLGTVIGVMPGLGPPAT
                     IAMLLPLTFLMDPASAMIMLAGIYYGAKYGGSTTSILLNVPGESASVATCFDGYQMAR
                     KGRAGAALGIAAIASFIAGTVGVLGLMLIAPPLAKMALAFSSPEYFALMALGLAMVVL
                     LAGRSMVKALLAMLVGLWLAGIGTDLFSQTSRFTFGQMELLSGVDFVVVAIGVFAIGE
                     VLTNMEGREAAKALPVPKGLRNLLPTLQDLKDSRFAFLNGSVVGFLIGVLPGAGSTIA
                     SFISYGLEKAVSRRPEEFGKGVVEGVAAPEGANNAETGGALIPMLTLGIPGSSTTAVL
                     LAALVLWGFKPGPLFIPENPALFWGLVASMYIGNVMLLVLNMPLVPLFAQVLRTPAYI
                     LYPLILGISVIGVYSASGSLFDVWLLAGFGLLGYLMRKLDYPSAPLILGLVLGGAMER
                     ALRQSLMMSDGSLSILVSRPVSAVMLSLTLLILLIPLFNKFNTWRLKALDN"
     gene            complement(21335..21766)
                     /locus_tag="VIG70_14380"
     CDS             complement(21335..21766)
                     /locus_tag="VIG70_14380"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY8251786.1"
                     /translation="MRAAKADFWSGLALGGLGAYIIVTASRWEYLGADGPGPGFFPLW
                     FGIAMAGLSLLLMVFSVKEKHGETISWSGAGRAFSAWAALALSVAALKLVGFLVSFAA
                     LTFFIVAVMYRRPYRVAAVVGIAGAAGFYVVFALALGVKLP"
     gene            complement(21766..22158)
                     /locus_tag="VIG70_14385"
     CDS             complement(21766..22158)
                     /locus_tag="VIG70_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8251787.1"
                     /translation="MNRIVHLALKVEDLERTTEFYQKVFGFKEMNTEKVRDHTSRHLS
                     DGALDIALIKYDAGTQSAESKAAGEGPCIHHFAVEVDNVAAATKQVKAYGCEIISDPG
                     VIPVKFRAPGGTVCELVPKGRYKKPKSK"
     gene            complement(22155..23528)
                     /locus_tag="VIG70_14390"
     CDS             complement(22155..23528)
                     /locus_tag="VIG70_14390"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY8251788.1"
                     /translation="MKQDAISPVMRKLSSYIAEARRRPLPEKVVERTKHHVLDTIAAM
                     VSGSRLLPGRKGIAYVKTLGGTKEACIIGSRFVTNAANAALANGMCAHADETDDSHAP
                     SVMHPGCGIVPAALAVAEKEGRSGEALLRAVALGYDVATRLTMSLNAFKFREDGLSTH
                     SYGPTFGAAAAAGLLVGVNQEQARYLLSYAAQQASGISCWMRDEQHIEKAFDFGGMPA
                     RNGVTAATMVAQGFSGVDDVFSGERNFFVAHGRPANPEEMVRGLGEDYEILRTNIKRW
                     SVGSPIQAPLDSLLELIKSNAVKAEQVEKLTIRVAHRDKNTTDNRPMPDINMQHLCAV
                     MLFDGNVTFKSSHDEKRFRDPKVREFRKRIDVQGDDALTKTMPARQAIVEIALRDGRN
                     LRHHTVAVRGTAENPMTRDEVDAKSYDLIAPVTGGARSRKLCDAVWKLEGLKDVRKLR
                     PLLQEKP"
     gene            complement(23525..24292)
                     /gene="fabG"
                     /locus_tag="VIG70_14395"
     CDS             complement(23525..24292)
                     /gene="fabG"
                     /locus_tag="VIG70_14395"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY8251789.1"
                     /translation="MPSNVGMKPGTELAGKVALVTGGARNIGRAIACSLAAGGASVMV
                     NARTSRAEAEQTVEMIGSSSAFHIADVTKPEEVKALVEATVKRFGRLDFLVNNAAVRY
                     ETAFSAISFDEWRRVLAIVLDGAFLCAQAVLPHLLRAGGGSIINIGGQTGHRGASERA
                     HVITAKAGLAGMTKALALDLAPHQITVNCVVPGTIDSQRGLPGVPDRPAHRRAPPPIG
                     RRGEPEEIAAMVRMLCGPDARYITGQAIHVNGGGYLP"
     gene            complement(24303..>24774)
                     /locus_tag="VIG70_14400"
     CDS             complement(24303..>24774)
                     /locus_tag="VIG70_14400"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY8251790.1"
                     /translation="LRVTVMAFLVIFGSATFGQVLAFTGASSGLMNWAIGFKVTPLGM
                     LFIMIGVIVFLGSFMDPLSKMLLTAPIFFPLAKTLGFDLTWFGLIMLLALEIGYTTPP
                     FGLLLFVMKGVAPPGTTMRDIYLAGMPFIACVVALIGLIIAFPPLATWLPAISR"
BASE COUNT         3945 a         8355 c         8187 g         4287 t
ORIGIN      
        1 gcaccaggag ggcggccacg ttttcggcca aaaactctcc caggtgcggc gcatctgcca
       61 atagaacttt ggaaattccg gaaatggccg aggctgcctt cgcggcttcc cccgcctgat
      121 gcccggcaat caggacgtgg atgtccgagc cgagcttctg cgccgcagcc acggtgttga
      181 gcgtggcctt cttgaccgtc ttgttgtcgt gctcggcgac gacgaggacc gccatcagat
      241 gaccttggct tcgttgcgca gcttgtcgac cagcgccttg acgtcggcca ctttcgtgcc
      301 ggccttgcgt ttgccgggct ccacgacctt cactgtcgcc aaccgggggg caacgtcgac
      361 cccaagcgcc tcgggcttga gcacttcgag cgtcttcttc ttcgccttca tgatgttcgg
      421 cagggtcacg tagcgcggct cgttcaacct caagtccgta gtgaccactg ccggaatctt
      481 gattgaaacc gtctccaggc cgccatcgac ttcgcgggtg acctgcgctt tctcgccttc
      541 gatcttcagc ttggaggcga aggtcgcctg cggccatccc agcagcgcgg ccagcatctg
      601 accggtctgg ttggagtcgt cgtcgatcgc ctgcttgccc aggatcacga gcttcgggct
      661 ctccttctgg cagacggctt tgagaagctt ggcgacggca agcggctgca gctcggccgc
      721 ggtctcgacg aggatggcgc ggtcggcgcc gatggcgagc gcggtcctca gggtctcctg
      781 gcaggactgc gcgccgcagg aaacgaccac gagctcggtc gccaccccgg cttccttaag
      841 ccgtaccgcc tcctccaccg cgatctcgtc gaaagggttc atcgacatct tgacgttcgc
      901 ggtctcgaca cccgtgccgt cggccttgac gcgcaccttg acgttgaaat cgataacgcg
      961 cttgacggcg acgaggactt tcatgggagc cggaaattat aatacccggg ccatgagcga
     1021 cctttcccaa ctgaacatca agtccatgcg cgagcgcgtt tcaaacgagg aatggcaggc
     1081 tcgagtcgac ctcgccgcgt gctaccgcct ggtccaccac tacggcatgg acgacctcgt
     1141 ctacaaccat atctcggcgc gcgtgcccgg cgaggaaggc catttcctca tcaacgccta
     1201 cggcatgatg tacgacgaga tcacggcttc gagcctggtg aagatcgact tcgacggcaa
     1261 cgtggtgcag gactcgggca ccggctacgg catcaaccag gccggcttcg tgatccacag
     1321 cgccgtgcac aaggggcggc cggacgtcgg ctgcgtgatc cacacgcact cgatcgcggg
     1381 catggcggtg tcggcgctcg attgcggcct gctgccgatc acgcagaccg cgatgtactt
     1441 cgacggcgtc ggcatgcacg agtacgagag cgtggcgatc gacctcgacg agcagcagcg
     1501 cctggtgcgc gatctgggcc gcttcaaggc gatgatcctg agaaaccacg ggctgcttgc
     1561 cgtgggtccg acggtttcgg aagccttcac caatctcttc tggctggagc gcgcgtgcaa
     1621 ggcgcaggtc gcggcgatgg cgtgcaacgc caagctccac ctgccggcgc aggacgtgat
     1681 cgagaaaacc aaccacctct accggcccga gacacgccgg cgctggggcc cgctcgagtg
     1741 gccggcgctg ctgcgcctgg ccgatcgcaa gtaccccggc ttccgcgatt gaagcccctc
     1801 cagggtagaa gcgtcatcgt caccggcgca ggcggcggca tcggccgcga cttcgcgctc
     1861 gccatggccg ccgccggcgc caaggtgctc gtcaacgacc tcggcacgtc ggtgaggggc
     1921 gaaggacgcg atgcaggccc cgctcagcaa gtggtcaacc aaataaagga aaaaggcgga
     1981 atcgcggtcg ccaacaccga cagcgtcgcg gagtgggaat ccgcgaacag gattgtttca
     2041 tccgccgtcg aggcgtttgg gcgcctcgac gtagtggtga acaatgcggg gattctgcgc
     2101 gaccgcttct tcttcaacat gtcggtcgag gaatggcgcg cggtgatcga cgtgcatctc
     2161 aacggctcgt tctacgtgtc gcgcgccgcg gcgccgcact tcaagtcgca gaattccggc
     2221 tgctacgtgc acatgacgtc gacctcgggc ctggtcggca acctcggcca ggcgaactac
     2281 tcggcggcca agctcggcat cgccggcctg tcgaagtcga tcgcgctcga catggccaaa
     2341 tacaacgtgc gctcgaattg catctcgccc ttcgcctgga gccgcatgat cggctcgatc
     2401 ccggccgaga ccgacgacca aaaggcgcgg gtggaaaagc tgaagtcgat ggagactgcg
     2461 aagattgcgc cgttggcggt cttcctggcc tcggaagagg cgcaggacgt caccggccag
     2521 atcttcgccg tgcgcgccaa cgagatcttc ctcatgtcgc agagccggcc gctgcgctcg
     2581 gtgcaccgcg cccagggctg gacgccggag acgatcgctt ctcatgcgat cccttccctg
     2641 agagcgcact tctatccgct ggaccgttcg caggacgtat tttcctggga tccgtcgtga
     2701 agcctcgcgt cctgcttacg gacgcgatcg cgcccgcggg cgaggcgctg ctcgcccgca
     2761 cggtcgaggt ggtgcgcgcc ccggatagcg agccctcgac gatgcggcgc tgcgcgcgcg
     2821 acgccgacgg ggtgatcatc cgcagcaagc tgccggacga catcttcgag gccgcgccgc
     2881 ggctgcgcgg cgtggtggtg catggcaccg gaaccgacct ggttccggtt gccgccgcca
     2941 ccgagcacgg catcatggtg gcgaacctac ccggcatcaa cgcgcaatcg gtggccgagt
     3001 actgcgccct ggcaatgctg atgctcgcgc ggaacattct ttcgatcgtg agctcgctga
     3061 aaagcgaggc ctgggacgcc gcccgcgtgc gcggcgcggg cgcgcacgag atcgccggca
     3121 tgacgctcgg catcgtcggc gtcggcgaga tcggcgggcg cctggcgaag atcgcgcggc
     3181 acggtttcgg catgaacgtg ctcggccacc agcgccgact ggatcgtgtg cctgccgagg
     3241 cctccccagt ttctctcgaa gagctgctcg caaagagcga tttcgtggtg ctcgcctgcc
     3301 cgctgacgcc gcagacgagg tacttgatga atgcaagaac catcggcttg atgaaaccgg
     3361 cggcctggct gatcaacgtc ggccgcggtc cggtggtgca ggaagaggcg ctgatcgagg
     3421 cgctgcgcgg gaagcgcatc gccggcgcga tgctcgacgt gtacgagcac tatcgcctgg
     3481 agccggggca tctcctgttc acgctcgaca acgtgatcct cacgccgcac ctggccgcgg
     3541 tcacccaaga gtcgcgcgcc cgggcgggcg tcgccgcggc ggacgagatg ctgcgcatcc
     3601 tgcggggcga gcggccgcgc aatctcgtga atcccgaagt cctgaagaaa gagcagctga
     3661 agacatgaat ctcgggcgcg aaagccatcc cgagctgcgc gacgcggttc gccagctctg
     3721 cagccagttc gactcggcgt attggcagaa gatcgacgag aaacgcgcct accccgaggc
     3781 cttcgtggac gcgctgacca aggcgggctg gatgtccgcg ctcattccgg aggaatacgg
     3841 cggctccggg ctctcgctca ccgaggccac ggtcatcatg gaggaagtga cgcgctcggg
     3901 cggcaacgcg ggctgctgcc acggccagat gtacaacatg aacacgctgc tgcggcacgg
     3961 ctcggcgcag cagaagaaaa aatacctgcc caagatcgcc tcgggcgagc tgcgcctgca
     4021 gtcgatgggc gtcaccgagc ccggcgcggg caccgacacg acgaagatca agacggtggc
     4081 gaagaagaaa ggcgaccgct acctcgtgaa cgggcagaaa gtgtggacct cgcggctgca
     4141 gcattcggag ctcatgatcc tgctcgcgcg cacgacgccg ctcacggagg tgaagcgcaa
     4201 gagcgaaggc atgtcgatct tcctggtcga gacgaagagc aagggcctcg aggtgaagcc
     4261 gatccgcaac atggtgaacc acgagaccaa ctcggtgttc atcgacaacc tcgagatccc
     4321 cgctgacaac ctgatcggcg aggaggggaa gggcttccgc tatattctcg acgggctgaa
     4381 cgcggagcgc atcctcattg ccgccgaatg catcggggac ggatattggt tcgttgaaaa
     4441 atcctgctcc tactcgaaag accgcgtggt gttcgaccgc cccatcggcc aaaaccaggg
     4501 gatccagttt ccgatcgccg aggcgtacat ggagatcgag gcggcgaacc tgatgcgcta
     4561 caaggccgcc atgctgtttg attcccacaa accctgtggg gcagaagcaa atatggccaa
     4621 gcacctggcg gcgaaggcgt cctgggaagc ggccaatgtg tgcatgcaga cctacggcgg
     4681 cttcggcttc gccgcggaat acgatgtgga gcggaaattc cgagagaacc ggctatacct
     4741 cgtggcgccg atctccacca acctgatcct gtcctacctc gccgagcacg tgctcggcct
     4801 gccgcgctcc ttctagaacc gcctcattgc aggatgtggc cggtcttgct gaggatggcg
     4861 aactcgacga actgcgatcc ggtctgcttg ggctgggtga aatcgacgaa gtagccgccg
     4921 aggtcgacgc tcttcatccc gcgcaccgcc ttcgccagcg ccgccgggct ggaacgcgcc
     4981 tgccgcagcc cgtggaccag caccttcgcc gcgatgtatc cctccatcga gcgctccgcc
     5041 aggtcgggat cctttgccgc ctgcgccagc tgctgatatt cacggatcag ccggaagctc
     5101 ctcgccgtgg taaggggcca gaggaccccg aagccgtagc cgctgagctc gtcgccgctc
     5161 aggctgcgct tcatggcgtt gatatcgatg accgagacgc cgtagatctg cgccgagccg
     5221 ccgccttgcc ggtactgctt gatgaactcc accgcggccg aggtcacggt gccgaggtag
     5281 atcacctgcg cgtcggactt gagcaccgcc tggacggcgg cccctacgtt tgtcgtgttg
     5341 cgctcgtacg gcgccttcac taccagattc acgcgcgtcg ccggagcggc cctttcggcg
     5401 ccggcaatga tgtccttgcc gaagccgtcg tcctggtaca cgaccgcgac gcggttgatg
     5461 ccgatgagag acaggatctc gaacaggcgc cgcacctcgt ccccatagct cgccttggtg
     5521 acgaacacgt tgggcgcccc gatcatcgtg ctcgcgcccg agatcgcgcc gaccagcggc
     5581 acattcgcgc gcgcgaggac gccgtccttg atcagggctt cgttgttcgc cgtccccagt
     5641 acgccgatga acgcgatcgg cgcttccttg gcgagaactt ccttcaccag acggaccgtc
     5701 tcctcggcct tgtagccgtc gtcgtaggtc gccagcaaga tccgctcgcc gccgattccg
     5761 ccgttccggt tcacgtggtt caggtacaga ttgatcccgg cgcgaatcgc cttgccgctg
     5821 ctcgcctggg ccccggtgaa agcggccacc tgcgccacca tgatttcccc acccgacgcc
     5881 tgcgccgaag caagcaggca taccgccgtc gcgattgctg cgatccgcat gttcgcgttc
     5941 tccctggatc cgaagacgcg agcatgcgcc gtcggtcagg aaaacacgcc gggcaaattc
     6001 acggattctt gcaatcggac gtggcgaatg gcctaggttc gcggcgccgt gatatttgcc
     6061 actctagaag cccatgtcgc gagcgcttgg gcgttcatat ttactcgccc gtcctgccgt
     6121 aagcgtgtgt catgcgggtg tgcgctatcc gcagaagggc ttcacgctga tcgagctgat
     6181 gatcatcgtg gggatcatcg gcatcctttc ctcactcgtg ctcccggctt atcaggatta
     6241 ctcggtgcgc acgaagatgt cggaggtggt cctggcgctg acctcgtgcc gggtcagcat
     6301 caccgaggtc taccaaatcg gccccgacac cgcccccgga ccgaacggct ggggctgcga
     6361 agccggcgcc actccgggaa cgacgtacgt ttcgtccgtc acgacgactg ccgatggcgt
     6421 caccagcgcc accgtacgcg gcataacgac ggcggtgaac ggcgcggtag tcacgctggc
     6481 gccgctcgcg ccgcctgcgc ccggaacacc cgcgaccttc gtgccgggta cgagccagcc
     6541 gctctatggc tggcgctgcg gcagcgctgc ggatggcact actgtcgcgg cgcgctacct
     6601 gccatcgtcg tgtcgcggct gagctagcgc tgcgcgagtt gtcggttggc ctgagcgaga
     6661 cgctcgacca gcacgcgcat gaaggccttg ttgaagccca cctggcaggc ggcggtggcc
     6721 acgcgcagcg cgccggcctt gatctgcatc accgtgatgc ggtcgcgcgc cgtcaccgtg
     6781 gtggtgcggc gctcgacgct gtcggcgaaa tacaggatct cgccgaagca gccgccgggc
     6841 ttgagagtgg caagcggctt gccgaggagc gagacctcga cttcgccgtc gaccagcacg
     6901 tagaggctgt cgccctggtc gttctcgcgc atcagcaccg tgccggcctc gagcgtctgc
     6961 caggtcgcga cgcgcaccac ttcccacagc tcgacctcgc cgaagtcctt gaagaaagac
     7021 atgtcgcgca gcttgccgaa cttctcggag tcggacgcgg tggcgtcggt cggccgcagg
     7081 ctcatgaagg cgcgcgtcac ggccttgccg aactcgagcc acgagcggta gcgctcggcg
     7141 gggttcctcg cgatcgcctt catcacgatc tcgtcgagca gcggcggcag gtcggggcgg
     7201 aagctcgacg ggcgcggcgg cggcgagtgg aggatggcgt aggtcagcgc gacatggctg
     7261 cccgcctcga agggcaggcg cccggtcagc aggcggaaca tggttacgcc gagcgagtag
     7321 atgtcggtct gctgggtgag cttctccatg cgcagctgct cgggcgacat gtaggccggc
     7381 gagccgacgc ccttgagctg cgtggtcttg tcgtccaggc gctcctggaa cgacgcgccg
     7441 aagtcgccga ccttggtctc gccgagctcc gacagcagca cgttgcccgg cttgatgtcg
     7501 cgatggatga tgccgtgctg gaacgcgtac tcgagcgcgc ggatgcactt gaagatgatc
     7561 tcgaccacct tggagagggg cagcaggctg gtgggcgtgg cgtggctgtc gagcgtcgtg
     7621 cccggcacgt actccatcac caggtagctg cggtccgggt cgaccaccgc gtcgtagatg
     7681 tccacgatat gcggatgatt cagcttgccg gcgagcgacg cctcggcgac gaacgccttg
     7741 tgcaccatgc gctcggtatc tcgatcgacc cgccgggcga actgcagcac cttgatggcg
     7801 acttcgcgct cggcgaacgg atcgtgagcg aggtagaccc ggctggtggc gccgctgccg
     7861 atctcgcgga tgaccgggta ctttccgatc atctgctggg cctgcggccc ttcgaccttc
     7921 acgcggtgta cttggcgacc agctgcagca gctcggcctc cggatagggc ttgccgagga
     7981 acgcattgac cccgatctgg gccgcctggt tacggtgctt ctccgcggtg cgcgacgaga
     8041 tgaagatgat cgggactccc tgcgtgcgcg cgtcggcgcg cacgtggcgc gccaggtcga
     8101 agccgtccat gcgcggcatc tcgatatcca cgagcatcac gcggggcagc gattcggcca
     8161 ttttctcgag cgcgtcgacg ccgtccttcg cggtgaggac gcggtaaccc tcgcgctcga
     8221 gcaggcgccc ggtgatcttg cgcaccgtga gggagtcgtc gaccaccatg accaccggcg
     8281 cgcgcgcggc ccttatcgac gtactgtccc tcagcgaccc cctcgtgtcc ctcagcagcg
     8341 acgtcgtcgt gaacatgcta cgcgcagcgg ccggagcggc gcgctgccgc tgggcgagct
     8401 gcaccgggtt catgatgagc gcgatgcggc cgtcgccgag gaccgtcgcc ccggtgatgc
     8461 ccgggacgcg gccaagctgc gcgccgatgc tcttcaccac gatttcctgg ttgccgagca
     8521 gctcgtcgac gtgtaccgcc acgcgctgca cgccgctgcg cagcagcaac accgagttgt
     8581 agggatgcag ctcgccggcg gacgccctgc ccagcagctg ctgcatcgca tgcagggggt
     8641 aggcgcgatc ctggaaatcg atcgacttct tctcgtagct caacgccagc gcgtccgact
     8701 tcagcctcag gacctgctcc accatcgcgg ttgcgagcgc gatcacgttc tcgccgctgc
     8761 gcaccagcac cgcctgggta accgccaggg tgagcggcag gtacacggta aacgtggtcc
     8821 cccgtccctg cgcggtgagg atgtcgacgc ggccgccgat aagcgatatt tcgttgcgta
     8881 ccacgtccat gcccacgccg cggccggcaa cctcggtcac cgcctcggcg gtcgacaggc
     8941 cggaggcgaa cacgagttgg gcgatctcgc cttcggtcgg ctcgcggtcg gcgggcagga
     9001 ggcccatcgc ggcccccctg cgacgaagcg cttccaggtt caggcccgcg ccgtcgtcgg
     9061 agaagatcag cgcaatctcg ttgctttcct ggcgcagcgc gatcgcgatg cgcccgctct
     9121 cggctttgcc ggcggcggcg cgcgcggccg gatcctcgat cccgtgcacc agggcattgc
     9181 gcagcatgtg ctcgaggggc gcgctgatgc gctcgagcac gccgcggtcg agctcgacct
     9241 cggcgccggt gatctcgagc tcggccttct tgccgatctc gcgcgcgatc tggcgcacga
     9301 tgcggtacag gcgctcgctc aggttcgaga acggcaccgc gcgtacgcgc atcaggtcct
     9361 gctgcacctc gcggctggtg cgcgcctggt gcaggagcgc agcgtcggtg tcgccgatgt
     9421 acttgtgcag gccctgctgg atcgacatca cgtcgttcag gctctcggcc atcagccggg
     9481 tgagctcctg caggcgcgta tagcggtcga actcgagcgg gtcgaactcg cccttcttct
     9541 cgtccatcac cgacaggcgc gactgcatct ggctgtcggc ctggacttcg acctcgcgca
     9601 gctggttgcg caggcgcgcg atgctctccg tgagatcggc gagcgactgc ttgatcgccc
     9661 gcagctcggc ttcggctcgt gtgcgcgcga tcgcgatctc gcccgcttcg ttgatcaggt
     9721 gatcgagcgt gtcggcgttg acccgcagca tcgccgccgg ggtcggcagc ggcgcctcgg
     9781 cgcgcggctg cggctgcact tccgtggtcg acggcgagaa cgccggggct gcgggcgcag
     9841 gcgcggccat gcgctcgacg tccgtgctga gccggtccat ccgctcctgg aggtactcga
     9901 agaggtcggc cggcaacgcc ccgcccgcct cgagcgcgga ctcgatgcgg ccctccatca
     9961 ggtggcacag ctcgcccagc cggatcgccc cggccatgcg cgcgctgccc ttcagcgtgt
    10021 gcagcgagcg gcgcagcgac tgcgagatct tcgcctccga cgggttcgcc ttccactcgc
    10081 gcaggtcgga gccgatgtcc ggtacgagct gttgcgcttc ctcgaggaag atcggcagca
    10141 gctgcgcgtc gatgtcgtcc agcatgcggc gcttttcgcg cccgtcggcg ggcgccgccg
    10201 cgggtgccgg agggggggcc accgcaggca ccggggctgc ggccgggaca ggctctggga
    10261 gcgtagtgcc ggcacggtgc gcagccagcc gcgccgattc gaggcggcca atgagctcgc
    10321 gcagggtgtc ctgcggcatc tggctgatcg ccggcgcgtc tccccggctg atcgcttcga
    10381 ccatgatgcc caggtcgacc accgcggcct gcacagtctc ggcgtcggca gcgtctgtcg
    10441 tctgctcggc gaacacgctc cactcttcga gcgagcccgc cagctcggcg agcgggatga
    10501 acccggccgt atgcgaagtg ctccccagcg tgtgcgccgc gcgcatgaaa tcggcggaca
    10561 ccggtcccgg cgcggtctcg cgccacttgg cgcactccgc gcgcagcacc gcaatatgct
    10621 ccgccgcttc cttggtatag atgtcgtaga ggttgcgcgc tacggtgacc gttcccaccg
    10681 tgaccgttgg aacggcaacc gcttcggctt gggccgcttt cgcccacgcc gcaagctcga
    10741 cgatcttgct gtcgtcgatc aggccttcca ggcccggccc gcgcagcttc gcgatccatt
    10801 cggagaacga gccggccgcg gtctcgagca gctcgaggag cgccggcgtc accgcgcgct
    10861 tctgctcgag ccaggcgttc atcacctgct cgacctgcca ggccgcctcg cccaggtcca
    10921 tcaggccgac catgcggccg ctgcccttca gcgtatggaa cgcgcgccgc accaccgtct
    10981 gcggctcgcg atcctcgggc ttctcgcggc attccggcag cgtggtcttg atggtctgca
    11041 gaacctcctc ggcctcttcg aggaagacct ggagcagctc gccgtcgacc ttcgggcgtg
    11101 gcccggtgtc gaccttgggg cgcggcgcgg cttcgacctt cgggcgcgcg ggcgctgccg
    11161 caacgggcga agtcctcgag ggaaactcga gccgcagcgt gctgtcgatc gtcgcctgca
    11221 cgtggcgctt ctcgtaacgc cggaaaaaca gcgcgatggc ttcctcctgc gggtcctggc
    11281 cgtggcggca gggctcgagg aagaatccga cgctgctcag gccttcggcg atccagtcca
    11341 tgtcgccggg cccggtccct tcgccgcgcg cctgcgcgag gatcaggctc tcgcagatcg
    11401 acacggcctg cgccgcgccc gggaagccga gcacgcttag cgccccgtgc atctgacgca
    11461 gatagggctg gaggtcagcg agcgtcgcgc gcttcgtcgc gtcgcgggcg aacgcatcga
    11521 tcacctgctc gctgtgctgc aggttggcga ggatttcctt cgcgacctgg gcgcgcagct
    11581 gcagcgcgcc gatgcgctcg gtgagatcgg ggcgcaggcc agccggcggc tcgcccgtcg
    11641 atttgccttt ggcggcgtcg agcagccacc cgcccatgat gacgatctgc gcctcgaggt
    11701 cctccgccgg gctgctgaaa tggtcgatca catgctcgac cagcaggaag gccgacgcca
    11761 tctcgatcac catcatctgc tcctgccgcg gatagggctc gggcagcttt cccgcgacca
    11821 gcgcgatcgc atcgagcagc ttgatcagct gctggttgcc gagatccttg gtcttcgtct
    11881 tgaagtcggc gacccgctcg cggaaggcgc gcgccttgtc gcgctcgccg gcgacgtact
    11941 gctcccacgc ccgcttgagc gcgtcgaggc gggaatgcat gtcgaacagc gacgcttcga
    12001 ggcgctcggc gtcgagctcc tgcacggcgg ccggcttctc ggcgcgcgga aacaggctct
    12061 cgagctgata gagctgcttc acctgcttga cgcgtgccgt gacgacctcg cgcttcgcaa
    12121 tcgaatacag cacctcgcgc aggagcgcgt cgccgttctt ggccgcccct gccgccaggt
    12181 cgcggatctg gaagtcgagg cggttgcaca ccaccttcgc cgcggcgagc cattgcgggt
    12241 cggcgctcgc cggctcgacc gccagcgact cgatcagcgc gccggccgcc caccagagag
    12301 cgcgcggccc gggcagctgg gtggccacct ggtaaagcgc gtccgtggtc tggcgcattt
    12361 cctccagccc ggccgggcca ccgcgcagcc aggccagcaa tccgcgctgg aaccgcgcgc
    12421 gctgggcctg caggaacgcc ggcagctcgt cttccggcat cgccttcata ctggtatgcg
    12481 gcggcgccgg ctggctcagg tccggataga aaagatcctt ttccgaaatg gcgctcttgc
    12541 cgaccaggcc ggccacgtcg cggtacaccg ggaacaggcg catcggcacg tcggtgtgcc
    12601 cgcgcgcgag ctcgccgacg aagttcttga gcgcgagcac cgcgcgctcg atgacgtcca
    12661 tcgcggccgg cgtcggccgc gacgcgccca tgctggcaaa gctcccctcg atcgcctcgc
    12721 acacccgtgt cgcgccgctc agcccgacca tgcgcagcgc cccgctcacc tggtgcaggt
    12781 gctcggggca aggctgcagc accgcgtcgt tgtccggcgc gacggcgaac ttggcgatcg
    12841 actcctggac cagcgtgagg gcgcggtcga cctcggcgtt gatccaggaa aggagcgtgg
    12901 tgtcggtcat ctagagatca aggcgctttt cttgccgttc tactaagcgg cgagcttgaa
    12961 gccggccacg gagctcttca ggccatcggc gagcgcggtg aggcgggccg ccgaagtggc
    13021 ggtctgcttg gtgccgtcgg tggtgagctg ggtgatgccc agaatcacct tcatgttgga
    13081 cgcgaccttg ccggcggagg tggcctgctg ctgggtcgcc gccgagatcg actcgatcag
    13141 ctcggcgagc tgccgcgaga ccgtgccgat ctcgcgcagc gcctgatccg cctcgtcggc
    13201 ggtcttcgtc ccctgcacca cgccctgggt gctgcgctcc atggcgtcga tcgcttcctg
    13261 ggtgtcgcgc tgaatgctct tgacgatcgc gccgatgtgc ttggtcgcct cggccgagcg
    13321 ctcggcgagg cgctgcactt cctccgcgac caccgtaaag ccgcgcccgg cctcgccggc
    13381 ggaagccgcc tggatcgccg cgttcagcgc gagcacgttg gtctgctcgg tgatgtccga
    13441 gatcagctgc acgatctcgc cgatctcctg cgagctctcg ccgaggcgct tgatccgctt
    13501 cgacgtctcc tggatctgga cgcggatgtc gttcatgccg cggatcgcgt tctgcaccgc
    13561 ctgcgcgccc ttctgcgccg cgtgcagcga gctttccgcc accttggagg agtcgttcgc
    13621 cttcttcgat acgtcgctca tcgacaccgc catctgggcg accgactggc cagcgccctg
    13681 gatctcgacc gcctgcttgc tcgccgcctg cagcagcttg ccgctgaccg tctcggcttc
    13741 ctgcgtcgcg ctcgtcacct gcagcgaggc gctggtgatg cccgcgacca ggcgccgcag
    13801 ctcgtcgatg gtgtagttga tcgaatcggc gatggcgccg gtcatgtgct cggtcacctg
    13861 ggcgcgcacc gtgaggtcgc cgttcgccag gttgccgatc tcgtcgagca gccgctggat
    13921 cgcctgctgg ttctcgcggt tctgccgctc gctttcctcg gcgcggcggc cgctgtcatc
    13981 gaggtacacc ttgccgatga gcagcagcac gaccagcgcg agaagcgaca ccgcggcgag
    14041 cacgatgaag ttgaggcgcc ggccgacgag ctcggcctca taggcggtgt tcaggttctc
    14101 ggcggcgccc agcagctctt cgctgtcctt gaacagctcg aagctcgcgc gcttggcgtt
    14161 caccagctgc tgctggttgc cgaggatgct gctgacccca gcctggtact ccttgaagct
    14221 ggcttccacc tcggcgagct tgccgcgcag ctcggggtcg gcggcgcgct ggatgcgcag
    14281 cgcctcgctg ccggcgagca gcccctgcag gatgtcgcgg aagctgttgg cgtccttgcc
    14341 gagcaggaac gacacttccg ggtcgatcac gtcttcggcg agcatggtat tggcgttttt
    14401 cgccatgcgc tgcgtcagca tcatgagctg cgacaccagg gcgttctggc gcgccgagcc
    14461 gccgctctgc acgctgagcg cggcgacctc gtcggtgagc tcctgcagca ccgggctcct
    14521 cgcgttgatc gcgcgcaccg ccttgccgag gttgagcagg ttgttctgct cgacgatcac
    14581 cagcgccgcg ttgcgctcgt tcttcttcca gtgcgcattg agcgtttcga gcaccggacg
    14641 cacgcccgag gaggtgggcg gcagcgcgac gccggccgac gcgccgccgg cgagcagcag
    14701 gttcatctgc gacgcgaatt cgtcgcggct ctcgcgcagc tgcttgaagg cctcgaggtt
    14761 gccctgcgac gcctgctgcg cggctttcgc gagccgctgc gacagcatgc ggatcttgcc
    14821 ggcggtggca atgtagatcg tgccgaaggt ggcctgccgc gcatcgaacc acacgaccac
    14881 ggcgtcgatg gcgagcagcg ccaccagcag cccgatcagg atttgcagcc ggcgtccggt
    14941 gctgagtttg ccgaagagat tgagtgccat gatgtgcctg cctgtgttga gcctgcctgg
    15001 gttaactcat taacgaagaa atctgccgtc gcgcgagatg atcgtcaggt cgacgaacct
    15061 ggagcccgcg cggttggtgg gcgaatagcc gatgaaaaag ccgccgagat cgacgtcctg
    15121 catcttctcc agcgagtcga ccaggccttc gcgcgtcggg ttcttgcccg cgcgcttcag
    15181 gccctcgacc atcaccttcg cgctgaggaa cccctcgagc gcgctgaagt tgtagtcggt
    15241 gtaccccgac ttcttcgcga gctgctggta ttccttgacc accggcaccg aggtgcccca
    15301 ggggaacggc acgacctggg aaatggcgac gccgacgccg tcctggccga gcgcgtcggc
    15361 gagcgccttg ctgccgacga aggagacgtt gtaaaacgtc gccgcgctgc cctgcttctg
    15421 catcgcccgc acgaaagccg cgatcgacgt ataggcgctg accatgacca ccgcgtcggg
    15481 ctgcgaagcg tggatcgcct tcgccgccgc ctcgacctgg acggtgttgc gctcgaccgt
    15541 gccctgcgag tggatcttca gcttgcggcg ctcgagcgcg agctcggtgc ccttgcggcc
    15601 ggcctcgccg taagcgtcgt tctggtagaa cactgcgatc ttgcgcccgc cggtcgacac
    15661 cacctgctcg acgatcttct cagtttcgtc gtagtagctg gcgcgcacgt ggaacacgta
    15721 gcggttgaac ggcgcgcgca gcacctccgc gccggtgaac atgcccacca gcggcacccg
    15781 ggcctgggtc acgaccggca gatgcgcgac cccggtcggg gtcccgacat agccgagcag
    15841 cgcgaacacc cggtgctctt cgatcagctt ccggctcgcc ccgggagcga ccgaggcctc
    15901 gtagcgatcg tcttcggtga gcagctcgag cttgcggccg tacacgccgc ccttcgcgtt
    15961 cacgtgatcg aggtaggtct tgatcccgtt gcgcatctgg atgccgagct gcgccgccgg
    16021 tccggtgaat accgccgcct gcccgaggaa gatcctttcg gaggtaacgc cgggctctgc
    16081 tgccgcaatg gccggcagcc aggttgcgag gatacaaaga atgcgtcgca tggttgctcc
    16141 cttcacagtg caaccgtcag gaattcggcg tcgcgcacca gctcggcgac gttgagctcg
    16201 ctccagcggc cgccgccctc gtcttcgtac tcgccgcgca gccaggcggc gcgccccggc
    16261 gccgcctcgc gcagccgcag gtgcgccgga ttgcgcagcc cgagcgagcg gtcgacgagc
    16321 agcgcggcgg cgctgcggaa gcgctcgccc aggagcacca ggcgggcgtc ctcgccggtc
    16381 ccggagatgc cggtgccgag gaactcggcg aaatcgacca cgctgtagag gttgccgcgc
    16441 acgttcgcca cgcccctgaa ccaggggcgc gtcagcggca ccggggtgat cgggggcacc
    16501 cgcagcacct cgctcgcctc gaccaggtcg atgagccagt tctgctcgcc gacctgcacg
    16561 ccgagcttgg acgcgctgcg cgcgctatcg gccagccgca ggcgctccgc cagctcgcgc
    16621 tggtattcgc ggagggaaac gcgcgcgcgg gtaattccct gttccatcag ccgagcgccg
    16681 aaatcttctt gagcagctct gggcctttca ccggcttgac gatgtagtca cgcgcgccct
    16741 gcttcatgcc ccaggcccgg tcggtctcct ggcccttggt ggtgcagatg atgaccggaa
    16801 tggtcttcgt ggcttcgtcc ttgctcagcg cccgcgtcgc ctggaagccg ttctgccccg
    16861 gcatcacgat gtccatcagc accaggtctg ggcgcatctg tttcgccttg gcgagcgctt
    16921 catcggcgtt ctgtgcggtc tcgaccttgt agccgttctt cgccaggaga tcggtcaggt
    16981 actggcggtc ggttgcggag tcgtcgacga cgaggatgtt cttgatcggc attgctggac
    17041 ctttctgagt tctagggaga gggtttgaga tgttccgtca ctgcttccac caatccctcc
    17101 ttggtgaagg gcttggtgag atagcggtcg gagccggcga gccggccgcg cgcgcggtcg
    17161 aacacgccgt ccttgctgga cagcatgatc accggcgtcg tcttgagctg cgggtgctgc
    17221 ttgatgagcg cgcaggtctg gtagccgtcg agcctcggca tcatgatgtc gacgaagatg
    17281 acttgcggct ggtggtccga aatcttggcg agggcgtcga agccgtcttc ggcgaggatc
    17341 acttcgtagc cggcctggcg caggaacagc tcggcgctgc gacgaatggt gttgctgtcg
    17401 tcgatgacga gcaccttgat agcgcctgtt gccgccactg ccactcgggg atctcccctc
    17461 ggaataagtc gctcacgcac cttgtgggtg ctcgggagtt ctcgccggtt atttatgttc
    17521 tcgcgggtca cacaacccgc ttcggcgttc tcccgccttc aggaaaacgt ttgtactcta
    17581 gctcttagag agcgtttctc gtgttaacta ttgcattggc cgagggggcg gcgcaactcg
    17641 accttagact tttactttta ttgcgatgca gcatgtggtg tcgctacatg ctcgggtcgc
    17701 acccccatgc cgatcagacg gcccgggagc cgtcgcagca acgcgaaccg gttgacccag
    17761 cggaagaaaa gcggcggcga gagcggcccg gtacttccta ggacccgggt gatgatgcgg
    17821 tcctgcgcca ggatctggag gcgctgggtg acgcggaccg gccactcgcg ccggcgctgg
    17881 attttcttca gcttctgtat tgaaaagtct ttctcgctca ggatgttggc cgccgcgacg
    17941 gcgtcctgga tggcgaggtt gatgccgacc cctccgaccg gcgacatggc atgcgcggca
    18001 tcgccgatgc agagcagtcc cggccgccac cactgcttca gccggtcgat ctgcaccgtc
    18061 agcagcttca cctgctccca gtccgcgatc tctgcgacgc gtggggcgac ccagggcagg
    18121 agccgcgcca gggtggcgcg aaacgcgggc agtccggcgc cgcgcacgct atcggccgcg
    18181 cccttcggaa tgacatagcc gcactgccag tacgcgcgcc ggttgatgag aacgagcgcc
    18241 gcgccggtgt cgaagcgtcc catgctcgac tgcggatcgt ccgggtgcct cgagagacgg
    18301 aaccacagca cgtcgatcgg cgcgcccagg gtctccacct cgagcccggc cgacctgcgc
    18361 acggtggagt tgcgcccgtc tgcggcaatc accaggtctg cttcggcttt ttcatcggaa
    18421 aacctgagtc cggcgacccg gcccttgctc tcgatcaagc cggtgacctc ggcgttcatg
    18481 cgcaactgga actgcgggta gcgcgccgcc tcgcgcgcga ggaagtcgag gaaatcccac
    18541 tgcggcatca tcgcgataaa gccgcagcgc gtcgggaggt ggcggaagtc ggcgatcttg
    18601 agcgggtagt cgccgaactg agccgcgatc tcgggcacgc ggtcgtgcgg cagctcgagc
    18661 agccgctcga gcaggccgag ctcggcgagc acctcgagcg tcgacgggtg cagcgtgtcg
    18721 ccgcggaagt cgcgcaggaa gtcggcgtgc ttctcgagca ccagcgtctc gatgcccgcg
    18781 cgcgcaagca gcaggccgag catcatgccg gccgggccgc cgccggcgac gacgaccttc
    18841 acgccgtcag cggacggtct tgacgcccgc ctcctgcagg atgccgcgca tctggccggt
    18901 gaagtcgtcg tagaacttcg tcagctcggc ggcgttctgg taggcgtcct cgaactggtt
    18961 gtccgcgacg tacttcttcc acgattcggt ctggctcatc ttcttgaaca cgccttccca
    19021 gtaggccacg ttctctcgcg gaattcccgg cggtgcgacc acgccgcgca cctgcgggac
    19081 cggcgtgacg tcgaagcccg cctccttgag cgtgggcacg ttcgcgtatg ccgcgaggcg
    19141 cttgtcgttg acctgcgcga gcaggcgcat gttgccggcg cggatgtgct cgcccgcctc
    19201 gttcggctcg atcaccatca tgttgacgtg gccgccgagg agcgcggcga tgcgctcgcc
    19261 gccgccgggg aacgagatga actgccagcg cgccccgctg accttttgca gctgctggcg
    19321 caccacgttg tcgcggctcg tgatggagcc gcccgactgc ttgagctcgc ccggcttctg
    19381 ttttgcggcg ctcacgaaat cgcccagcgt cttgtagggg gcgtcggcct tcaccgcgat
    19441 gaccgccggc tcgagcagca ggcgggcaat cggggtcagg tccttcagcg acaccttggc
    19501 ctcgttgctg gtgagcgggt tggtgagcca caccccggtg aagatgccga tggtgtgggt
    19561 ctcgcctttc ttctcggcga gatacgccgc agccaccacg ccgccgccgc cgggcttatt
    19621 tgcgacctgc gcgcggaccg gaagcagctt ctccttctcc atcatcaaca ccatcgcgcg
    19681 cgccaacacg tcgctgccgc cgccggggcc ggtgtgaacc acgacttcca cgggtcttgt
    19741 gggagtaaag ccctgcggca tcgccggcaa cgccaccgca agtcccagga cagccaaggc
    19801 aaccctcttc atcgcatcct cctcgtgcta attgtcgagc gctttcaggc gccacgtatt
    19861 gaacttgttg aacagcggaa tcagcaggat cagcagcgtc aacgatagca tcacggccga
    19921 gaccggccta gacaccagga tcgagaggct gccgtccgac atcatgagcg actggcgcag
    19981 cgcccgctcc atcgcgccgc ccagcaccag gccgaggatc agcggcgccg aggggtagtc
    20041 gagcttgcgc atcaggtagc cgagcaggcc gaacccggca agcagccaga cgtcgaacag
    20101 gctgcccgag gcgctgtaaa ccccgatcac cgagatgccg aggatcagcg ggtacaggat
    20161 gtatgccggc gtgcgcagca cctgcgcgaa cagcggcacc agcggcatgt tgaggaccag
    20221 cagcattacg ttgccgatgt acatgctcgc caccaggccc cagaacagcg ccgggttctc
    20281 gggaatgaac agcggtccgg gcttgaagcc ccagagcacc agcgccgcca gcagcacggc
    20341 ggtcgtggac gagcctggga tgcccagcgt cagcatcggg atcagcgcgc cgccggtctc
    20401 cgcgttgttg gcgccttcgg gtgcggccac gccctcgacc acgcccttgc cgaactcctc
    20461 cgggcgacgc gacaccgcct tctccaggcc atacgagatg aacgaggcga tagtggatcc
    20521 ggcgccgggc agcacgccga tcaggaagcc gaccacggag ccgttgagga aggcgaagcg
    20581 cgagtccttc agatcctgga gcgtcggcag caggttgcgc aagcccttcg gaaccggcag
    20641 cgccttcgcg gcctcgcgcc cttccatgtt ggtgagcacc tcgccgatcg cgaacacgcc
    20701 gatcgccacc acgacgaagt cgaccccaga cagcagctcc atctgcccga aggtgaaacg
    20761 cgaggtctgg ctgaagaggt cggtgccgat accggcgagc cacaagccga cgagcatcgc
    20821 gagcagcgct ttcaccatcg agcggccggc aagcagcacc accatggcga ggccgagcgc
    20881 catcagcgcg aagtactcgg gcgagctgaa cgcgagcgcc atctttgcga gcggcggcgc
    20941 gatcagcatt aggccgagaa cgcccaccgt gccggcgatg aaagaggcga tggcggcgat
    21001 gccgagcgcg gcgccggcgc gccccttgcg cgccatctgg tagccgtcga agcaggtcgc
    21061 caccgaggcg gactcgcccg gaacgttcag caggatcgag gtcgtcgagc cgccgtactt
    21121 cgcgccgtag tagatgccgg cgagcatgat catcgcgctc gccgggtcca tgaggaacgt
    21181 cagcggcagg agcatcgcga tcgtcgcggg cgggccgagg cccggcatca cgccgataac
    21241 ggtgcccagc accacgccca ggaagcacca cagcaggttc atcggctgca gcgcgaccgc
    21301 gaatccctgc atcagccccg agaggacgtc cacgctaagg aagcttgacg ccgagggcaa
    21361 gcgcgaacac gacgtagaac cccgccgcgc cggcgatgcc gaccaccgcc gccacccggt
    21421 aaggccgccg gtacatcacc gccacgatga agaaggtgag cgccgcgaac gaaaccagga
    21481 acccgacgag cttgagcgca gccaccgaca gcgccagcgc cgcccaggcg ctgaacgcgc
    21541 ggcccgcccc ggaccaactg atggtttcgc cgtgtttttc tttcacgctg aaaaccatga
    21601 gaagaagcga gaggccagcc atggcgatcc cgaaccagag cgggaagaag cccgggccgg
    21661 ggccgtcggc gccgaggtat tcccagcgcg acgccgtcac gatgatgtac gcgcccaggc
    21721 cgccgagcgc gagtccggac cagaagtcgg ctttcgccgc cctcatcact ttgatttggg
    21781 tttcttgtaa cggcctttcg gcacgagctc gcagaccgtg ccgcccgggg cgcggaactt
    21841 cactggaatc acgccggggt cgctgatgat ctcgcagccg taggccttca cctgcttcgt
    21901 cgcggcggcg acgttgtcga cttcgaccgc gaaatggtgg atgcagggcc cctcgcccgc
    21961 cgccttcgac tccgccgact gcgtgcccgc gtcgtacttg atcaacgcga tatcgagcgc
    22021 gccgtcgctc aggtggcgcg aggtgtggtc gcgcaccttc tcggtgttca tctccttgaa
    22081 gccgaacacc ttctggtaga actcggtggt gcgctcgaga tcctcgacct tgagcgcgag
    22141 atgcacgatg cgattcatgg cttctcctgt aacaggggtc tcagcttgcg aacatccttc
    22201 aggccttcca gcttccacac cgcgtcgcac agcttgcgcg agcgcgcgcc tcccgtcacc
    22261 ggcgcgatga gatcgtagct cttggcgtcg acctcgtccc gcgtcatcgg attctctgcg
    22321 gtgccgcgca ctgcgaccgt gtgatggcgc aagttgcgcc cgtcgcgcag ggcaatctcg
    22381 acgatcgcct gccgggccgg catggtcttg gtcagggcgt cgtcgccttg cacgtcgatt
    22441 cgtttccgga attcccggac cttcggatcc cggaagcgct tctcatcgtg cgaggacttg
    22501 aacgtcacgt tgccgtcgaa cagcatcacg gcgcacaggt gctgcatgtt gatgtcgggc
    22561 atcgggcgat tgtcggtggt gttcttgtca cggtgcgcca cgcggatggt cagcttctcg
    22621 acctgctccg ccttcaccgc attgcttttg attagctcca ataaagaatc cagcggggcc
    22681 tggatgggcg aacccaccga ccagcgcttg atgttggtgc gcaggatctc gtagtcctca
    22741 cccaagcccc gcaccatctc ctcgggattg gcggggcggc cgtgggcgac gaagaagttg
    22801 cgctcccccg agaacacgtc gtccacccca ctgaagccct gcgccaccat ggtcgccgcg
    22861 gtgacgccgt tgcgcgccgg catgccgccg aagtcgaacg ccttctcgat gtgctgctcg
    22921 tcgcgcatcc agcaggagat gccggaggct tgctgggctg cgtacgacag gagatagcgg
    22981 gcttgctcct gattcacgcc gacaagcaat cctgccgcgg cggccgcccc gaaggtcggg
    23041 ccatagctat gggtcgagag gccgtcctcg cggaacttga acgcgttgag cgacatcgtg
    23101 agccgcgtcg ccacgtcgta gcccagcgca accgcacgca gtaacgcttc tccactcctg
    23161 ccttcttttt ccgccacggc aagggcggca ggcacgatgc cgcagcccgg gtgcatgacc
    23221 gaaggcgcgt gcgaatcgtc ggtctcgtcc gcgtgcgcgc acatgccgtt ggcgagcgcg
    23281 gcgttggctg cgttggtgac gaatcggctg ccaatgatgc acgcctcctt cgtgcctccc
    23341 agcgtcttta cgtaggcgat gcccttcctg ccgggcagca ggcgcgagcc ggagaccatc
    23401 gccgcgatcg tgtccaggac gtggtgcttc gtcctttcaa cgaccttttc cggcaagggc
    23461 ctgcggcgcg cttcagcgat gtacgagcta agcttgcgca tcaccggcga gatggcgtcc
    23521 tgcttcacgg caggtaaccg ccgccgttga cgtggatcgc ctgcccggtg atgtagcgcg
    23581 cgtccgggcc gcagagcatg cgcaccatcg cggcaatctc ctcgggctcg ccgcggcgac
    23641 cgatcggcgg cggcgcacgc cggtgcgcgg gacggtcggg cacaccgggc aatccgcgct
    23701 ggctgtcgat cgtcccgggc actacgcagt tgacggtgat ctggtgcggc gcgaggtcga
    23761 gcgcgagcgc cttcgtcatg ccggcgaggc cggccttggc ggtaatcacg tgcgcacgct
    23821 ctgaggctcc cctgtggccc gtctggccgc cgatgttgat gatcgagccg ccgccggccc
    23881 gcaggaggtg cggcaacacc gcctgcgcgc agagaaacgc gccatcgagc acgatggcca
    23941 gcacgcggcg ccattcgtcg aaactgatgg ccgaaaaagc ggtttcgtaa cgcacggcgg
    24001 cgttgttgac gagaaagtcg agccgcccga agcgcttcac ggtggcctcg acaagcgcct
    24061 tgacctcttc gggcttcgtc acgtccgcga tgtggaaagc ggagctcgag ccgatcattt
    24121 ccacggtctg ctcggcctcg gcgcgcgaag tccgggcgtt caccatgacc gacgcgccgc
    24181 cggcggcgag cgagcaggcg atcgcgcggc cgatgttgcg cgcgcccccg gtcacgaggg
    24241 cgaccttgcc ggcgagctcg gtgccgggct tcattccgac gttgctgggc aaacgctttc
    24301 tcctagcggc tgatggcggg caaccacgtg gcgagcgggg gaaacgcgat gatcaggccg
    24361 atcagcgcca cgacgcaagc gatgaacggc atgcccgcga ggtagatgtc gcgcatggtg
    24421 gtccccggcg gggccacgcc cttcatgacg aagagcagca ggccgaaggg cggcgtggtg
    24481 tagccgatct cgagcgcgag cagcatgatg agcccgaacc aggtgaggtc gaagccgagc
    24541 gtcttggcga gcgggaagaa gatcggcgcg gtgagcagca tcttggagag cggatccatg
    24601 aagctcccca ggaacacgat gacgccgatc atgatgaaga gcattccaag cggcgtgacc
    24661 ttgaagccga tcgcccagtt catcaggccg ctggaggcgc cggtgaacgc gagcacctgg
    24721 ccgaacgttg ccgagccgaa gatcaccagg aacgccatga ccgtgacgcg tagc
//
LOCUS       DATBSZ010000230        27811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2159543, whole genome shotgun sequence.
ACCESSION   DATBSZ010000230 DATBSZ010000000
VERSION     DATBSZ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 27811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27811
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2159543"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VIG70_14405"
     CDS             <1..444
                     /locus_tag="VIG70_14405"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY8251791.1"
                     /translation="LLRDTLAKAGKAGIASVVIRTKQYLAALVAQDELLVLNTLRYAD
                     ELKDPAELKIPEARVTAKELDMAMRLVEDMADEWRPSRFKDTFRADLLKRIKEKVKKG
                     ETEEITEAEKEPRAAKSAEVIDLMQLLKKSVEKKQPKRAKRRRAA"
     gene            456..668
                     /locus_tag="VIG70_14410"
     CDS             456..668
                     /locus_tag="VIG70_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251792.1"
                     /translation="MGTCEVCGNQYEKAFSVKMPDGGAHTFDSFECAIEKLAPRCAHC
                     KCRIIGHGVDNDATGKIYCCEHCAHA"
     gene            661..1749
                     /locus_tag="VIG70_14415"
     CDS             661..1749
                     /locus_tag="VIG70_14415"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8251793.1"
                     /translation="MPKRQIRYAVVGLGHIAQVAVLPAFGNARRNSKLAALVSGDSLK
                     RKSLSKKYEVENAWSYEQYDECLETVDAVYIALPNSLHCEYAVRAARAGVHVLVEKPM
                     ALTEEECERMTHAARDAGVKLMVAYRLHFERANLEAGEVARSGRIGEPRLFNSTFCTP
                     VVPGNIRAKRDAGGGVLYDIGIYCINAARALFRDEPVEVRATAAGTLGEVEESVSAIL
                     RFPNDRMASFTASFGAAKVSEYRLSGTKGDLAVEPAYDYARPLKHRLTLDGEMRERRF
                     AKRDQFAPELLYFSDCVLQNGTPEPGGDEGLADVRVIRALYRSAATGQPVALEPFAKR
                     ARPSLEQEIRRPPVEKPEVIHAAAPSGG"
     gene            1789..2145
                     /locus_tag="VIG70_14420"
     CDS             1789..2145
                     /locus_tag="VIG70_14420"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8251794.1"
                     /translation="MMKTLIAAAVAATFAVPVFAQKAEDKQSLPTAQERAGNRQEVSR
                     SAAGAALTTKVKTALASDAGLKTVTSIDVDSADGVVTLKGKVDSADMKKKAGDIAKKV
                     DGVKSVKNELRVEKKG"
     gene            2150..2380
                     /locus_tag="VIG70_14425"
     CDS             2150..2380
                     /locus_tag="VIG70_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251795.1"
                     /translation="MGDSGTPLLSVTQTRDGVWSVNERGPARVAIAYFPTKWGAMRHA
                     VRAARAQRRCRVAIVERGGGVRLSHDYTQETP"
     gene            2377..2892
                     /locus_tag="VIG70_14430"
     CDS             2377..2892
                     /locus_tag="VIG70_14430"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEY8251796.1"
                     /translation="MSTATFALIFGIAYLGAGLLGLVPAALTPPPADAPPTHVTFLYG
                     YLLGLFPVNVLHSAVHIAIGAWGLLAWRGTASPTVFARSLALFYGALALMGVIPGMST
                     LFGMLPMHGHDVWLHAGTALIAAYFGWRSAVSPERRASSAADRRQHMLPVERERRFGL
                     ADRRHMAYAGA"
     gene            2942..3472
                     /locus_tag="VIG70_14435"
     CDS             2942..3472
                     /locus_tag="VIG70_14435"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEY8251797.1"
                     /translation="MRTATFALFFGIAYLSAGVLGMVPAALMPAPPDAPPTKIAVLYG
                     YLLGLFPVNVVHSAAHFAIGAWGLAAWRSDHLGRGLGSPKTYARVLALFYGVLAVAGV
                     IPMMNTLFGLLPLHGHDVWLHAGTAAVAGYFGWRTEIVVERRGGNGPDRREKVIPVEH
                     ERRLGHADRRIPGSEV"
     gene            3509..4609
                     /locus_tag="VIG70_14440"
     CDS             3509..4609
                     /locus_tag="VIG70_14440"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY8251798.1"
                     /translation="MKPPFRADHVGSLLRPPELKQAREDFKAGRISRQSLAETENKSI
                     RKAVALQEAAGLQSVTDGEFRRAFWHVDFLTGFDGIVATQGQYALKFHGEGGAESETR
                     SMMVVESRVRRTRPVMVEHFKFLKQATKKTAKLCIPAPTYLHMRGGRRVVNESAYPDV
                     EEFWSDITQAYRQEIADLAKAGLEYLQIDDVSFACLCDEGIRAQVKRDGEDPAKLPAQ
                     YARIISGLIAERPESMRVTMHTCRGNHASMWMAEGGYDAVAEAVFQTEVDGFFLEYDS
                     ARAGGFAPLRFVPRNKKVVLGLVSTKTPVLEKKDALKKRVEEAAKYVALENLCISPQC
                     GFASSEVGNKLTEDDQKRKLELVAETAREIWG"
     gene            4640..5812
                     /locus_tag="VIG70_14445"
     CDS             4640..5812
                     /locus_tag="VIG70_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251799.1"
                     /translation="MRKLIIAAALATAFGAAHAQQTIKIGLVLSSSGQFADAGAQLDN
                     GIKTYMKMHGDTVAGRKIELIRRDTGGIAPDVAKRLSQELVVRDNVDILAGYVLTPNA
                     MAAGDVSAEAKKFMVVMNAATSVVITKSPYMIRTSVVLPQVMETFGTWAATKGGLKQT
                     YTMVTDYGPGHDSERAFTTGFTNAGGKVVGSVRFPVANPDFSAFVQRAKDIAPESIYI
                     FIPGGAQPAALGKAFAERGIDKKKTRVLGSGEATAEAALKAMGDNAIDIITAWHYDYK
                     LDNKLNAAFVKEFNAMHGRNPDFFSIGGYDGMHAIYETLKKTKGKTDGDTMIAAAKGL
                     KWDSPRGPMSIDPDTRDVVQTVYIRRVQKVGNELVNVPFDRVNEVKDPDGERRKKK"
     gene            5824..6687
                     /locus_tag="VIG70_14450"
     CDS             5824..6687
                     /locus_tag="VIG70_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8251800.1"
                     /translation="MLSSLATVLFDGFAYGMLLFLLSVGLSVTLGMMNFVNLAHCSFA
                     MVGGYATVTLVNGFGWPFLASLPIAFIAAALVSVVLERTLYRHLYRSTDLDQCLLTIG
                     IVFVSVAAFAWFYTSEQQSARPPEWLRGRIELFGVNFAAYRLFLILFGLAVTGLLIFL
                     LERTRFGAKVRAAVDNQRMARGLGIDVDRAFMLTFALGGGLAGLGGALAIEVVGLDPT
                     FALAFLVYVLIVVAVGGLGSIGGSFAAAVVLGISDMAGKYYFPWLGAFLIYLVMVGLL
                     MWRPLGLFGKR"
     gene            6674..7636
                     /locus_tag="VIG70_14455"
     CDS             6674..7636
                     /locus_tag="VIG70_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8251801.1"
                     /translation="MENASQHFREKVRWRWLEIAFWLCTLLPFVVTPNYLSLASQIAI
                     TALFALSLDLILGYAGIVSLGHAAFFGVGAYTAGLLAKHYWGEPLSGLVLAAAAAGLA
                     GYAMSHVIVRFRHLALIMLTLGLGLLLHEAANSASALTGGADGLQGVRVGTLFGFKFD
                     MFGRTAYAYSLAVLFVCFLICRRIIHSPFGLALRGIRENATRMPAIGAPSRAHLRKAY
                     TIAAVIAGVAGALLTHTTSTVSLEVLSFQRSADVLVILILGGAGRLYGGIIGAVIYMV
                     ARDQFSGINPQFWYFWIGLLLVLVVMFLPNGILGGISALYKKYQ"
     gene            7611..8345
                     /locus_tag="VIG70_14460"
     CDS             7611..8345
                     /locus_tag="VIG70_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251802.1"
                     /translation="MLSTRNINKSFGSLIVAQDVALELPKGVRYALIGPNGAGKTTLM
                     NLMTGMLAPDSGQIFLGGEEITGLRPDHRVKKGLARTFQINTLFPRLSALESVALAVL
                     ERDGHAHNWWRGLPRFGPAIDEAQSILASLKIPPTKETRELPYGQQRLLEIALALATR
                     PRVLLLDEPAAGVPKDESAALFDAIAGLSRELTILFIEHDMDVVFRFATRIIVMVGGR
                     ILVEGAPDEIARDARVREVYLGKAHA"
     gene            8338..9027
                     /locus_tag="VIG70_14465"
     CDS             8338..9027
                     /locus_tag="VIG70_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251803.1"
                     /translation="MLELKGVRAGYGEAVVLDDISLEVPANGSLAVLGRNGVGKSTLL
                     LTIMGYTRLHMGSILFNGADIGGMPPHRRAQLGIGWVAQEREIFPSLTLEENLTVASR
                     PGRWTLDAVYELFPRLKERKANMGNQLSGGEQQMLAIARALMTNPAVLLLDEPLEGLA
                     PIIIEELAVSLRNLDGIAMVLVEQHTEIALSLTQDAVVIERGRIVHRARSAELARDPA
                     MAERVLGLNVA"
     gene            complement(9007..9144)
                     /locus_tag="VIG70_14470"
     CDS             complement(9007..9144)
                     /locus_tag="VIG70_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251804.1"
                     /translation="MQRLLSGARSASPYLLVELLLPGGTLIALLLWLLRRKSGSGDVQ
                     A"
     gene            complement(9218..10489)
                     /locus_tag="VIG70_14475"
     CDS             complement(9218..10489)
                     /locus_tag="VIG70_14475"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY8251805.1"
                     /translation="MILLVAVFVALLVLGMPVAFSMALATIAGFIALGIPLETVAQKM
                     MSGIEPFPFVAIPLFVLAGALMETSGISRRLVALASAIVGHIRGGLGIVVVASEVLFS
                     GISGSSVADASAIGSILIPSLVKAGYPPERAAAIVAAASGMGILIPPCLVMVILGAMS
                     GLSISALFIAGFLPGILMALTLSIVIHIQARRGTLPGARGSFSGAALRHALREAILPL
                     GMPVVLFGGILGGVTTATEAAVLAVLYALVLDVVIYREITRKDLGRMLVQTGITTAAV
                     SVLAGFATGMSWTFATTGVPQMIANVLQQVTSSPFILLALVTLTLIVFASLLDGLPAL
                     IIFYPILSVTVAKYGIDPLHFGLLAVAALGIGLVVPPVGLLLVVVAQIGRVSLGAVTL
                     PMLPYIAILVATLLLIAYVPWVVLVLPRLLL"
     gene            complement(10486..10941)
                     /locus_tag="VIG70_14480"
     CDS             complement(10486..10941)
                     /locus_tag="VIG70_14480"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY8251806.1"
                     /translation="MFFHALEQALRAVVAALVVTIVVMVAGQVVFRYAVQEPIVWSEE
                     FTTLCYQWLSYLGAALAVRYRAHYGVDLFVRFLGPRWKRLLEWLEHAVIWSLAVFMVV
                     YGWRIVESSAVQMYPTLPFSVGTGYLVLPIAGALFVLMDWPVLMERRKP"
     gene            complement(10946..11923)
                     /locus_tag="VIG70_14485"
     CDS             complement(10946..11923)
                     /locus_tag="VIG70_14485"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8251807.1"
                     /translation="MRKFAFVAAAALFAASSAVAQTELKFADALPKTFAYYPAMEAFK
                     AVVDKTGKLKVELFPAGVLGDQKALMEATKVGAVDISVVASQVSQQLVPEHGIFGLPF
                     VWVKYDDWIRFLQGPIVAELGKKMEPHGLKVLVFADGGSLAVLNSKRPVRTPDDMKGL
                     KLRVMQDPMQVDMIKAMGAIPVPMGTPEVYSAIQQGQIDGNATGPQLLWALKNHEVAK
                     HLSFTNHGRASGVVLMNLKKWNSLNADQKKALDEGSKAFFKTDSEYFTSNPNTANDRV
                     VGFMANAGVTVTEPDIASFRKATMPVVQAFKNRVKSPLVDKALKEAGIP"
     gene            complement(11948..12259)
                     /locus_tag="VIG70_14490"
     CDS             complement(11948..12259)
                     /locus_tag="VIG70_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251808.1"
                     /translation="MKAKQPAVEAIRLLGQWQRVSRGHVVFAAGCSCGAAATSVRVQD
                     YEQDILSFLQKKHRTQATSIADLLTGLARHGGRGDLPILEDLERSIESFESAHRGGGG
                     S"
     gene            12288..14354
                     /locus_tag="VIG70_14495"
     CDS             12288..14354
                     /locus_tag="VIG70_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY8251809.1"
                     /translation="MTARTRLAADIGGTFTDVAAFDERSGQLLLGKTLSTPSRLVDGI
                     SHGVDKAGARFADAGLFLHGSTIAINTMLERTGARTALLITEGFRDTYEIGRINRPDS
                     YNLFFRKHVPLVERALRFEVRERMTATGEVLIPLDEAQVNGLCDRLEAAKVDAVAILL
                     LHCYVNPAHEERVKRLVQKRLPRAFVTASHELSQEYREFERCSTTVANAYIGPRVSRY
                     VAEIDAHIQKEGFEGAFLLVQSTGGLYQSRQAQTECVRMLESGPAAGVVGAQALCREL
                     GLKDAVAFDMGGTTAKAGVIYGGEVLTASTALVGGYNEALPIQIPMVHISEVGTGGGS
                     IAAVDAAGALRVGPQSAGADPGPACYALGGTRPTVTDANLVLGRLAADRFLGGEMRLD
                     EAAARRAITEHVAKPLGLGLADAASGILRIAVSSMSYAVKGVSTERGLDAAAFCLIAY
                     GGAGPLHASQIAREIGMSRIIVPRAPGHFCAFGMLHSDLRYDFVRTWFTRLEDAPFGE
                     IERIYERLIAQGKKALAESGVRFSRTTVAYAADMRYVGQEHPVTVDLAPTVFKKRDRD
                     AIKHHFDEVHQVRYGTSAPEERAEIVSLRATVTGLMKKPPLERISRGGAAPARAAQRG
                     TRTVFFSDKKLKTTAYARAALKAGNRISGPALIEEHASTTVLLPGDRMQVDSYGNLVI
                     EVGRKR"
     gene            14351..16105
                     /locus_tag="VIG70_14500"
     CDS             14351..16105
                     /locus_tag="VIG70_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY8251810.1"
                     /translation="MKRKKANADPILTEIVRNGVLAVTEEMKSNLMRTAYNMIIYEAL
                     DFTVGLFTADGRTVSIGMGLPSFIRGMANVVKAELEHFGPEGLEPGDILVTNDAYTTG
                     SHLNHFTFSMPIFERGKLCGWAACMAHWPDVGGTLGGVTTDIYSEGIQIPLMKYQKAG
                     VVNQELVEIIRMNVRIPERAMGDLRAQVIAVKTGERRFLELVRRYGREEVTAAIENIM
                     NNSEAAARARTKTIPDGTYEAESFMDDDGYTVGQRVPIKVRVVKKGDEMTIDLSEISP
                     QVQGFFNSGPSTGQSCAQVAYKCLTSPTDYPVNDGSFRSLKVIVPKGTVVSAIKPAAM
                     RWWMTFPMTVVDTVIKAVSKAIPQRTAAAHHADLVFALIHGISPRDGRFYIAACGPAG
                     GGWGARFNGDGMSGVVCSNDGDTHNSPCEQLEAKYPILFERFCLRTDSGGPGRFRGGL
                     GTEQVVQALAPITVDVQAERMHCAPWGIHGGMEGKGNHVTTRIDGKEDTDRANAKLRN
                     QRLKAGDALILRTGGGGGFGDPHERPAEVVANDVRQGYVSAEVARSAYGVALNADGGL
                     DTRATETLRKNKRVAESM"
     gene            complement(16116..16925)
                     /locus_tag="VIG70_14505"
     CDS             complement(16116..16925)
                     /locus_tag="VIG70_14505"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8251811.1"
                     /translation="MTDISANFSGSIPEYYDRCLGPAWFDQFAADLAARLAARPGGDV
                     LEIACGTGLVTRHLREKLAPGVRLVASDLSKAMLEYAKAQRGASGIEWREADACKLPF
                     GDAQFGAMVCAFGFMFVPDRKAAFAEARRVLREGGTLLFNVWDRVEENPHTLTSSQVF
                     EELFPGDPEMQFARITHGMYAPALLRELLAGARFREVRIEPRRMEVVCPDARTIATGM
                     IRGTPRVSLIGQRGKKVEDVIEHATAALAKLGGAAPFRSHAQAIVVEARAI"
     gene            complement(16922..17968)
                     /locus_tag="VIG70_14510"
     CDS             complement(16922..17968)
                     /locus_tag="VIG70_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8251812.1"
                     /translation="MKKTTNLKLDIFPHIFPLAYFERMKKIAEGNPGLAAQLKRWMHI
                     PVLWDLERRMKMMKRFPGYKQILTLSMPAVEFLGTPEETPALARLANDGMFEIVQEHP
                     DLFPAFVASLPMNNVPAALAEMDRAIAKLGAKGIQIFTNVNGRPLDDPEFFRIFERMS
                     KTYDLPVWVHPTRTAKFPDYLSEEKSKYEIWWLFGWPYETSAFMARLVFSGMFEKLPK
                     LKIITHHLGAMAPYFVNRIGYGMDQFGSRTSDEDYVSLLKSMKKRPVEFFKMFYGDTS
                     VNGSKSAIRCGLDFYGVGHVLFGTDCPFDPQGGPLFIRETIKALDGMKLKPNDRRRIY
                     FGNAMQMLKLKSMQ"
     gene            17991..18842
                     /locus_tag="VIG70_14515"
     CDS             17991..18842
                     /locus_tag="VIG70_14515"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8251813.1"
                     /translation="MSHSVRGALWMGGAVLSFTVMALAVRGLQARMGSFEIVFFRSVV
                     MLAIVLAMLPRAGMVSVLTRKFSLHLWRNLIHFVGQVLWVYSIGVLTLATVFAIEFTM
                     PVWASLLAWMFLKERLTFPRLVMLAFGLAGVLVILRPGAGSFHPAALAMVLGSLCYAS
                     SFIFTKRLTSTDSTLAVLFWMAVIQTPISAAAAAPQWVTPGWADVPWILGIGAGSFSA
                     HYCMTRAMKLVDAMVAVPIDFIRLPLIALIGAAFYGEALDPMVFVGAAVIFAGTYYSL
                     SRERRLP"
     gene            18919..19293
                     /locus_tag="VIG70_14520"
     CDS             18919..19293
                     /locus_tag="VIG70_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251814.1"
                     /translation="MAAEFIPLARAAALVHDRLFPGHGVKDAKTLDMIALALSALIPL
                     YQGDMEKGALIALEAGEIARGRFTRGATRLEFPDRAPLRFLLVSRRELYPAIDAIAKD
                     PTCPIWRLCRPRPPACSSATAA"
     gene            complement(19226..19855)
                     /locus_tag="VIG70_14525"
     CDS             complement(19226..19855)
                     /locus_tag="VIG70_14525"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251815.1"
                     /translation="MNLFRFLLVFFFLSTPVFAQDTGPEDLVKKITQDVLDTIKSDKQ
                     LAAGDRQKAIKLAEEKILPHVDFEEATRLAVGRGWKEASPEQKKKLVGEFRNMLVRTY
                     SNAISAYEGQTMKVMPVRMKPGDTDATVHNQFIRPGAKPVLVDYSMRKTEKGWKIYDI
                     VIEGVSLVLTYRSEFDAVVKQDGIDGLIKRLSQKNTPAAVGGTAAKSGK"
     gene            complement(19852..21237)
                     /locus_tag="VIG70_14530"
     CDS             complement(19852..21237)
                     /locus_tag="VIG70_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY8251816.1"
                     /translation="MANDNPRVLLVDDDKDLLQLIAMRLSATGYAVTAVESGEAALAA
                     LAVSRPQVVVTDLRMQGMDGMALFDAIHRDSPSLPVLILTAHGTIPEAVTATRRGVFS
                     FLTKPFEPKVLLDTVAEAMRLSSPPASGELETWRAELITRSSSMQDLLSQARRVAASD
                     ASVCIFGASGTGKELLARAIHRASPRAEAPFVAVNCGAIPEGLLESELFGHKKGSFTG
                     AVADRRGLFQAAQGGTLFLDEIGDMPLPLQVKLLRALEERKIRPVGSHEQYDVDVRVI
                     AATHRKLEERIASGEFREDLYYRLNVVKLYIPTLAERREDIPLLANHFLTRLAERYRR
                     GRLSLSPEATQILVSAPWPGNVRQLLNVIEQAVALAATEMIPESLVRQALDAGDTSLT
                     PLDEARRAFERDYLVRILKITGGNVTKAARLAGRNRTEFYRLLERHSLEPGMFKGNIK
                     GDKDHKERAAA"
     gene            complement(21286..22755)
                     /locus_tag="VIG70_14535"
     CDS             complement(21286..22755)
                     /locus_tag="VIG70_14535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8251817.1"
                     /translation="MIFYPRSFLALLLAGFTLVILPLVGALAYSAWNTERLAGKSRTA
                     VFNASQAARASRSLVDRIAAIERVAQQKTLLNDPELVTDYARVHRSFKQLAAEIAQLP
                     LDDAQAQALNVTVEREQGLYDLLTGSSLPRLDAQQVGAHIDALIDSARDVLAINNRVV
                     DREVERLRVSAEMVQRNIILLLIFSTAVALAIALALTRYIARPITEIDAAIRQLGGAD
                     FSQPIAVRGPEDLRYLGRRLDWLRRRLDEFETQKNRFLRHVSHELKTPLTALREGAEL
                     LNDQVAGPLAPPQRKVVGIMRDNSIKLQRLIEELLDYQRALHAAASLEVKPLQLAVVM
                     EEATHPHELAARAKGLRLVVDSESATVEADPEKLRSIVDNLISNAVKFTPPGGVVSVS
                     ARAVAGEAIIEVLDTGPGVPAEERESIFNLFFRGRRTEHATANSSTVRVKGSGLGLAI
                     ARELVEAHGGQIAVVAGGAGGHFRVTLPRRSARALADAA"
     gene            complement(22793..24112)
                     /locus_tag="VIG70_14540"
     CDS             complement(22793..24112)
                     /locus_tag="VIG70_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HEY8251818.1"
                     /translation="MDDRGYVAQALFDRLSGEGLRFRILGDSQGYPERAPPEVQIAVQ
                     AAALGGMPRAVARFCHDLDLQLVQLSPEDSRAWRCVLAWSDEVGRPRFMRVRICSDYC
                     RGARCYLRAEELLVGAPETLFSHGLVDAVESGELTAERAAWLSSLWNDDPRGALERIG
                     RFWPDRAQTRLVAQAARHGEWAAVRTSLAPLRRGLRRAQWPDPADLVARLAVAARNVL
                     EPARAAIAFVGRESPMRGEVMAQVAKDLAPLGLAVLDGTTRADLRVVFDPPGEFTGDD
                     LVVHAGRGLAANVVELERAILRWLECRVERRYPRALVGENPLAARVLQFAVRHGIPGV
                     QLFLNCDLRCRLGSPVLMPYPFGIVIERGARIGSRVTLMHQVTILGAVEIEDNVTIGP
                     GAKVIGPLRIGRGATIGPNAVVTEDVPSHETVVVEKRREIRRSVVNV"
     gene            complement(24112..25062)
                     /locus_tag="VIG70_14545"
     CDS             complement(24112..25062)
                     /locus_tag="VIG70_14545"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEY8251819.1"
                     /translation="MLQSRWRAVAFLLVAGPALAWAASALAPRQYVATGGVLVPAGMM
                     KVRYESTDPRAAATLVQGFIESHKEALLVDPPLVTSIAPNLGGNMALGAIAALLLSLF
                     LFGRRPRPVRSEGELIHALGEPLLAARPLAPQHLSRQLLAHWFAHGRRVLAVVSPQDG
                     AGGAHVAAELARAVAQSGEATLLIDADFRAPALHRAFGLRNRDGLADFLEGRGTQIAH
                     CAENLSVLVAGRSSADPLELLSRGRMLELLAAAATRYRVILVHTPAAARGPDLQLFAA
                     FAGGALVVAKRGSEAASLTRLRDLLALCKARVVGTVLSPV"
     gene            25253..26485
                     /locus_tag="VIG70_14550"
     CDS             25253..26485
                     /locus_tag="VIG70_14550"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY8251820.1"
                     /translation="MMKRGILAALLALGPTTAFAFETVDTLPFPSSGTFPGWPGDRGA
                     EPWSIYAYGGLMYDNNAFRRDTDEIDDIVARYGFGGRYATRVYGRQSVVLEAFGEYYD
                     YNHLSEIDHFGYGLRGDWLWELGNQASGNIGYVRRQRHADLGEFQVERRAMGTTDLAF
                     ADGGYRFLSDWRIFGALEYTRSSREGDEIGALEGKTVRTALTYRTPLGNVIGIDARAT
                     RGQAPFTDETTGAATSVHDFDERVIGATLAYSLGTQLRIGGRAGYTTREYDDLPQRDF
                     EGNTWRGGVDWLPTGKLVFRFETYHEPAPLTDLDSPFSIRTGALFSASWAATYKLVFT
                     ASFLNEHRVNESDPVAAVLGTPVRDETIRVWRFGVGWEVQRQWQLGAAIDFGDRTSNE
                     VGRDYEYQQVMLNARFTF"
     gene            complement(26535..27803)
                     /locus_tag="VIG70_14555"
     CDS             complement(26535..27803)
                     /locus_tag="VIG70_14555"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEY8251821.1"
                     /translation="MRCPSEPLVVCNETHRFLVQEQLRRAGARPKAIILESKGRGTAP
                     ALTLAAVHAVMADDPVLLAMPSDHFIAQPEAFQRAVERGAALARKGRIVAFGVPPASA
                     ETGYGYIRANAEYVEEFVEKPDAAKAAQYLASGQYLWNAGIFAVRASVWIDAIGNLRP
                     EILRACERAYRQGKRDGGFLRVDPAVFADCPADSIDYAVMENTSDAMVVRLDAGWSDV
                     GAWDALWGLEAKDADGNVIHGDVHAADTRNALLISQHRLLACVGLDDVVVVETADAVM
                     VARKDKAQAIGAMVASLKAAGRAECLAHRKVQRPWGSYDGIENGERFQVKRIVVDPGA
                     ALSLQMHYHRAEHWIVVRGTARVTRGDETFLLTENESTYIPPGTRHRLENPGKLPLEL
                     IEVQSGAYLGEDDIVRFDDAYGRANSGSGS"
BASE COUNT         4595 a         9481 c         9351 g         4384 t
ORIGIN      
        1 ctgctgcgcg acacgctggc gaaggccggc aaggccggca tcgccagcgt ggtgatccgc
       61 accaagcagt acctcgccgc gctggtggcc caggacgagc tgctggtgct caacacgctg
      121 cgctacgccg acgagctgaa ggaccccgcg gagctcaaga ttcccgaagc ccgggtcacc
      181 gcgaaggagc tcgacatggc gatgcgcctg gtcgaggaca tggccgacga atggcggccc
      241 tcccggttca aggacacgtt ccgcgccgat ctcctcaagc gcatcaagga gaaagtgaag
      301 aagggcgaga ccgaggagat caccgaggcc gagaaggagc cgcgcgcggc gaaaagcgcg
      361 gaggtgatcg acctgatgca gcttctcaag aagagcgtcg agaagaagca accgaaacgg
      421 gcaaagcggc ggcgcgccgc ctaaaggagc aagcgatggg aacctgtgaa gtctgcggca
      481 accagtacga gaaggcgttc tccgtgaaga tgcccgacgg cggcgcgcac accttcgaca
      541 gcttcgaatg cgcgatcgag aagctcgccc cgcgctgcgc ccactgcaag tgccgcatca
      601 tcggtcacgg cgtggacaac gacgccaccg gcaagattta ctgctgcgag cactgcgcgc
      661 atgcctaagc gacagatccg ctacgcggtg gtggggctcg ggcacatcgc ccaggtggcg
      721 gtgctgccgg cgttcggcaa cgcgcggcgt aactcgaagc tcgccgcgct ggtatccggg
      781 gattctctaa aaagaaaatc gctttccaag aaatacgagg tggagaacgc ctggtcgtac
      841 gagcagtacg acgagtgcct cgagaccgtc gacgcggtgt acatcgcgct gccgaacagc
      901 ctgcattgcg aatacgcggt gcgcgccgcg cgcgccgggg tgcacgtgct ggtcgagaag
      961 cccatggcgc tcaccgagga ggaatgcgag cgcatgacac acgccgcgcg cgacgccggg
     1021 gtgaagctga tggtcgcata ccgcctgcat ttcgagcgcg ccaacctcga ggccggcgag
     1081 gtcgcccgct cggggcgcat cggcgagccg cggctgttca actcgacctt ctgcacgccg
     1141 gtggtgccgg gcaacatccg cgcgaagcgc gacgccggcg gcggggtgct ctacgacatc
     1201 ggcatctatt gcatcaacgc ggcgcgggcg ctgttccgcg acgagccggt cgaggtgcgt
     1261 gcaaccgcgg ccggcacgct cggcgaggtc gaggaatcgg tgagcgcgat actgcgcttc
     1321 ccgaacgacc gaatggcgag cttcaccgcg tccttcggcg ccgccaaggt ctcggaatac
     1381 cggctgtcgg gcaccaaggg cgatctcgcg gtcgagccgg cgtacgacta cgcgcgcccg
     1441 ctcaagcacc ggctgaccct cgacggcgag atgcgcgagc ggcgcttcgc caagcgggac
     1501 cagttcgcgc ccgagctgct ctacttctcc gattgcgtgc tgcagaacgg cacgcccgag
     1561 cccggcggcg acgaagggct cgccgacgtg cgcgtgatcc gggcgctgta ccgctcggcc
     1621 gcgaccgggc agccggttgc gctcgagccc ttcgccaagc gcgcgcgccc gagcctcgag
     1681 caggagatcc gtcgcccgcc ggtcgagaaa cccgaagtga tccacgcggc ggcgccctcg
     1741 ggcggctgac gcgaaacttg ttttttcaac cccaaaggag caattgacat gatgaagacc
     1801 ctgatcgcag cagcagtcgc agccaccttc gccgtccccg tgttcgcgca gaaggccgag
     1861 gacaagcaga gcctcccgac cgcgcaagag cgcgccggca accgccagga agtgagccgc
     1921 agcgccgccg gcgccgccct caccaccaag gtgaagaccg ccctcgcctc cgatgccggc
     1981 ctgaagaccg tgaccagcat cgacgtcgac agcgcagacg gcgtggtgac gctgaagggc
     2041 aaggtcgact ccgccgacat gaagaagaag gccggcgaca tcgcgaagaa ggtcgacggc
     2101 gtgaagtcgg tgaagaacga gcttcgcgtc gagaagaagg ggtaacacca tgggcgactc
     2161 cggcacgccg ctcctctcgg tcacgcagac ccgggacggc gtctggagcg tcaatgagcg
     2221 cgggccggcg cgggtggcga tcgcgtactt ccccaccaag tggggcgcga tgcgccacgc
     2281 cgtgcgcgcc gccagggcgc agcgccgatg ccgcgtcgcg atcgtggagc gcggcggcgg
     2341 cgtcaggctc tcgcacgact acacgcagga gacaccatga gcacggcaac ctttgcattg
     2401 atcttcggca tcgcttacct cggggccggg ctgctcgggc tggtcccggc cgcgctgacg
     2461 ccgccacccg cggatgcgcc gcccacccac gtcacgttcc tctacggcta cctgctcggg
     2521 ctgttcccgg tgaacgtcct gcacagcgcc gtgcacattg cgatcggcgc ctggggcctg
     2581 ctcgcctggc gcggcaccgc gagcccgacg gtgttcgcgc gctcgctcgc gcttttctat
     2641 ggcgcgctcg ccctgatggg ggtgatcccc gggatgagca cgctgttcgg gatgctgccg
     2701 atgcacggcc acgacgtctg gctgcatgcc ggaaccgcgc tgatcgccgc gtatttcggc
     2761 tggcgctcag cggtttcacc cgagcgccgc gcgagctccg cggccgaccg gcgccagcac
     2821 atgctcccgg tcgagcgcga gcgccgcttc ggcttggccg accgccgcca catggcgtac
     2881 gccggggcct aaaagcaggg cagcaggaaa aaaagctaaa ctcccggcag ggagggagtt
     2941 aatgagaacc gcaacattcg cgttgttctt cgggattgct tatctcagcg ccggcgtgct
     3001 cggcatggtg ccggcggcgc tgatgccggc gccgcccgat gccccgccga ccaagatcgc
     3061 cgtcctgtac ggctacttgc tgggcctgtt cccagtgaac gtcgtgcaca gcgccgccca
     3121 cttcgccatc ggcgcgtggg gcctcgccgc ctggcggtcc gatcacctgg gacgcggcct
     3181 ggggagcccg aaaacctatg cgcgcgtcct cgccctgttc tacggcgtcc tcgccgtggc
     3241 gggggtgatc ccgatgatga acacgctctt cgggctcctg ccgctgcacg gccacgacgt
     3301 ctggctgcac gccgggaccg ccgccgtcgc cggatatttc ggctggcgca cggagatcgt
     3361 ggtcgagcgc cgcggaggca acgggcccga ccgccgcgag aaggtgatcc cggtcgagca
     3421 cgagcgccgg ctcggccacg ccgaccgccg gatccccggc agcgaggtgt agttctccgg
     3481 ggcggcttcc cactgtagtc tcgcagctgt gaagcccccc ttccgcgccg accacgtcgg
     3541 cagcctgctt cgccccccgg agctcaagca ggcgcgcgag gacttcaagg ccggcaggat
     3601 ttctcgccag tccctcgccg aaaccgaaaa caaatccatc agaaaagcgg tcgcgctgca
     3661 ggaagccgcc gggctgcaat cggtcaccga cggcgagttc aggcgcgcct tctggcacgt
     3721 cgacttcctc accggcttcg acggcatcgt cgccacccag ggccagtacg cgctcaagtt
     3781 ccacggcgag ggcggcgccg agtccgagac ccggtccatg atggtcgtcg agtcccgggt
     3841 aaggcgtacg cggcccgtga tggtggagca cttcaagttt ctgaagcagg ccacgaagaa
     3901 gaccgccaag ctctgcatcc ccgcgccgac ctacctccac atgcgcggcg ggcggcgcgt
     3961 ggtcaacgaa agcgcctatc ccgacgttga agagttctgg agcgacatca cgcaggccta
     4021 tcgacaggaa atcgccgatc tcgctaaggc ggggctcgag tacttgcaga tcgacgacgt
     4081 gtcgttcgcc tgcctgtgcg acgaaggcat ccgcgcgcag gtgaagcgcg acggcgagga
     4141 cccggcgaag ctgcccgcgc agtacgccag gatcatcagc ggcttgattg cggaaagacc
     4201 ggaatcgatg cgcgtcacca tgcacacctg ccgcggcaac cacgccagca tgtggatggc
     4261 cgagggcggc tacgacgcgg tggccgaggc ggtattccag accgaggtcg acggcttctt
     4321 cctcgagtac gacagcgcgc gcgccggcgg cttcgcgccc ttgcgcttcg ttccccggaa
     4381 caagaaggtc gtgctcgggc tggtgtccac caagaccccg gtcctggaaa agaaagacgc
     4441 cctgaaaaag cgcgtcgagg aggccgccaa gtacgttgcg ctcgaaaacc tgtgcatctc
     4501 gccgcaatgc ggctttgcat caagcgaggt gggcaacaag ctgaccgagg acgaccagaa
     4561 gcgcaagctc gagctggtcg ccgaaactgc acgagaaatc tggggttaga atccgacacc
     4621 actcttggag gagggaagaa tgagaaagct aatcatcgcc gccgcgctcg ccacggcgtt
     4681 tggcgcggcg cacgcgcagc agaccatcaa gatcggcctg gtgctgagct cgtcggggca
     4741 gttcgccgac gccggcgcgc agctcgacaa cggcatcaag acctacatga agatgcacgg
     4801 cgacaccgtc gccggccgca agatcgagct gatccgccgc gacaccggcg gcatcgcgcc
     4861 cgacgtggcg aaacgcctct cccaggagtt ggtggtgcgc gacaacgtcg acatcctcgc
     4921 cggctacgtc ctgacgccga atgccatggc cgcgggcgac gtctcggccg aggcgaagaa
     4981 gttcatggtg gtgatgaacg cggcgacttc ggtcgtgatc accaagtcgc cgtacatgat
     5041 ccgcacctcg gtggtgctgc cgcaggtgat ggagacgttc ggcacctggg ccgccaccaa
     5101 gggcggcctg aagcagacct acaccatggt gaccgactac ggcccggggc acgattcgga
     5161 gcgggcgttc accaccggct tcacgaatgc cgggggcaag gtggtcggct cggtgcgctt
     5221 cccggtcgcg aacccggact tctccgcctt cgtgcaacgc gccaaggata tcgcccccga
     5281 gtcgatctac atcttcatcc cgggcggcgc gcagcctgcg gcgctgggca aggccttcgc
     5341 cgagcgcggc atcgacaaga agaagacccg cgtcctcggc tcgggcgagg caaccgccga
     5401 agccgcgctc aaggcgatgg gcgacaacgc catcgacatc atcacggcct ggcactacga
     5461 ctacaagctc gacaacaagc tgaacgcggc gttcgtcaag gagttcaacg ccatgcacgg
     5521 ccgcaacccc gacttcttct cgatcggcgg ctacgacggc atgcacgcga tctacgagac
     5581 gctcaagaaa accaagggca agaccgacgg cgacacgatg atcgcagcgg ccaaggggct
     5641 gaagtgggac agcccgcgcg ggccgatgtc gatcgacccg gacacgcgcg acgtggtgca
     5701 gactgtgtac atccgccgcg tgcagaaggt cggcaacgag ctggtgaatg tgccgttcga
     5761 ccgagtcaac gaagtcaagg acccggacgg ggagcgcagg aaaaagaagt agctccgtag
     5821 aagatgctgt cttcgctggc gaccgtcctg ttcgacggct tcgcctacgg gatgctgctg
     5881 ttcctgctgt cggtggggct gtcggtgacg ctcgggatga tgaacttcgt caacctggcg
     5941 cactgcagct tcgccatggt gggcggctac gccacggtca ccctggtcaa cggcttcggc
     6001 tggccgttcc ttgcttcatt gccaatagcg ttcatcgctg ccgctttggt gtcggtggtt
     6061 cttgaaagga ccctgtaccg gcacctctac cgctccacgg acctcgacca gtgcctgctc
     6121 accatcggca tcgtgttcgt ctcggtggcg gcgttcgcct ggttctacac cagcgagcag
     6181 cagtcggcgc gcccgccgga gtggctgcgc gggcgcatcg agctgttcgg cgtgaacttc
     6241 gcggcgtacc gcctgtttct catcctgttc gggctcgccg tcaccgggct gctgattttt
     6301 ctgctcgaaa gaacgcggtt cggcgcgaag gtccgcgccg ccgtggacaa tcagcgcatg
     6361 gcgcgcggcc tgggcatcga cgtcgatcgc gccttcatgc tcacgttcgc cctcggcggc
     6421 ggcctcgccg gcctcggcgg cgcgctcgcg atcgaggtgg tgggcctcga cccgacgttc
     6481 gccctcgcct tcctcgtgta cgtgctgatc gtcgtcgccg tcggcggcct cggctcgatc
     6541 ggcggatcct tcgccgccgc agtggtgctc ggcatcagcg acatggccgg gaagtactac
     6601 tttccgtggc tcggcgcgtt cctcatctac ctcgtgatgg tcgggctgct gatgtggcgc
     6661 ccgctgggcc tgtttggaaa acgctagcca gcacttccgc gagaaagtgc gctggcgctg
     6721 gctggagatc gccttctggc tgtgcacgct gttgccgttc gtcgtcacgc cgaactacct
     6781 ttcgctcgcc agccagatcg cgatcacggc gctgttcgcg ctgtcgctgg acctgatcct
     6841 cggctacgcc ggcatcgtct cgctgggcca cgccgccttc ttcggcgtgg gcgcctatac
     6901 cgccggattg ctcgccaagc attactgggg cgagcctttg tccggactgg ttctggccgc
     6961 agccgccgct ggcctcgccg gctacgccat gagccacgtc atcgtgcgct tccggcacct
     7021 cgcgctcatc atgctcacgc tcggcctcgg cctgctgctg cacgaagcgg ccaacagcgc
     7081 gagcgccctc accggcggcg ccgacgggct gcagggcgtg cgggtcggaa cgctgttcgg
     7141 cttcaagttc gacatgttcg gcaggaccgc ctacgcgtat tcgctcgcgg tcctgtttgt
     7201 ctgcttcctg atttgcagaa gaatcatcca ctctccgttc ggcctggcgt tgcggggcat
     7261 ccgcgagaac gctacccgca tgccggcgat cggggcgccg agccgcgcgc acctgcgcaa
     7321 ggcctacacc atcgcggcgg tgatcgccgg ggtcgccggc gcgctcctca cccacaccac
     7381 ctccacggtg tcgctcgagg tgctgtcgtt ccagcgctcg gcggacgtgc tggtgatcct
     7441 gatcctcggc ggcgccggac gcctctacgg cggcatcatc ggcgccgtca tctatatggt
     7501 ggcgcgcgac cagttctcgg gcatcaaccc gcagttctgg tacttctgga tcgggctgct
     7561 gctcgtgctg gtggtgatgt tcctgcccaa tggaatcctg ggtggaataa gtgctctcta
     7621 caagaaatat caataagagc ttcggctccc tcatcgtcgc gcaggacgtc gcgctcgaac
     7681 tgcccaaggg cgtgcgctat gccctcatcg gcccgaacgg cgcaggcaag accacgctca
     7741 tgaacctgat gaccggcatg ctcgccccgg actcgggcca gatcttcctc ggcggcgagg
     7801 agatcaccgg gctcaggccc gaccatcgcg tgaagaaggg cctcgcgcgc accttccaga
     7861 tcaacaccct cttcccgcgg ctctccgcgc tggagtcggt tgcccttgcg gtgctggagc
     7921 gcgacggcca cgcgcacaac tggtggcgcg gcctgccgcg cttcggcccc gcgatcgacg
     7981 aggcgcagtc gattctcgct tcgctcaaga ttccacccac gaaagagact cgagagcttc
     8041 cctacggcca gcagcgcctg ctcgagatcg cgctcgcgct cgccacgcgc ccgcgcgttc
     8101 tgctgctcga cgagccggcg gcgggcgttc ccaaggacga gagcgcggcg ctgttcgacg
     8161 cgatagcagg cctctcgcgc gagctgacga tcctcttcat cgagcacgac atggacgtgg
     8221 tgttccgctt cgcgacccgg atcatcgtga tggtgggcgg ccgcattctg gtcgaaggtg
     8281 ccccggacga aatcgcacgg gatgcacgcg ttcgcgaggt gtatctcggt aaagcccatg
     8341 cttgagctga aaggcgtgcg ggccggctac ggcgaggcgg tcgtgctcga cgacatctcc
     8401 ctcgaggtgc cggcgaacgg cagcctcgcg gtgctcggcc gcaacggcgt gggcaagtcc
     8461 accctgctgc tcaccatcat gggctacacg cggctgcaca tgggttcgat tcttttcaat
     8521 ggagcggata ttggcggcat gcccccgcat cgccgcgcgc agctcggcat cggctgggtg
     8581 gcgcaggagc gcgagatctt tccctcgctc accctcgagg agaacctcac cgtcgcctcg
     8641 cgccccgggc gctggacctt agacgcggtc tacgagcttt tcccccggct gaaggaaaga
     8701 aaggcgaaca tgggcaacca gctctccggc ggcgagcagc agatgctggc gatcgcgcgc
     8761 gcgctgatga ccaacccggc ggtgctgctg ctcgacgagc cgctcgaggg cctcgccccg
     8821 atcatcatcg aagagctcgc ggtgtcgttg cggaatctcg acggcatcgc gatggtactg
     8881 gtcgagcagc acaccgagat cgcgttgtcg ctgacgcagg acgcggtggt catcgagcgc
     8941 ggccgcatcg tgcaccgcgc gcgctcggcc gagctggcgc gcgacccggc gatggcggaa
     9001 cgggtgctag gcctgaacgt cgcctgagcc gctcttgcgg cgcaggagcc acagaagcag
     9061 cgcgatcagc gttccccccg gaagcaacag ctccaccagg agataggggc ttgcgctgcg
     9121 cgcgccggag agaagtcttt gcatacgccg ccatgttgcg cggcgccctc tacccgagga
     9181 ttgcagtgcg tgaaccgccg gttacaaatg tgacgcgcta cagcagaagc ctcggcagca
     9241 ccagcacgac ccaggggacg tacgcgatca gcagcagcgt cgctaccagg atcgcgatgt
     9301 agggcagcat cggcagcgtg accgcgccga ggctcacgcg cccgatctgg gccactacga
     9361 ccagcagcag gcccaccggc gggacgacca agccgatgcc gagcgcggcg accgccagca
     9421 ggccgaagtg caagggatcg atgccgtact tcgccaccgt gaccgacagg atcgggtaga
     9481 agatgatgag cgccggcagg ccgtcgagca ggctcgcgaa cacgatcagg gtgagcgtga
     9541 cgagcgcgag caggatgaac ggcgacgacg tgacctgctg caagacgttg gcgatcattt
     9601 gcggcacgcc ggtcgtggcg aaggtccacg acatgccggt cgcgaaaccc gccaggaccg
     9661 acactgcggc ggtggtgatg ccggtctgca ccagcatgcg gcccaggtcc ttgcgcgtga
     9721 tctcgcggta gatgacgacg tcgagcacca gcgcgtagag caccgcgagc actgcggctt
     9781 cggtcgcagt ggtcacgccg ccgaggatgc cgccgaagag gaccaccggc atgccaagcg
     9841 gcaggatcgc ctcgcgcagg gcgtggcgca gcgcggcccc ggagaacgag ccgcgcgcgc
     9901 cgggaagcgt gccgcggcgc gcctggatat ggatcacgat cgagagcgtg agcgccatca
     9961 ggatgccggg caggaagccg gcgatgaaaa gcgccgagat cgaaaggccc gacatcgcgc
    10021 ccaggatcac catcaccagg cagggcggga tcaggatccc catgcccgag gcggcggcga
    10081 cgatcgcggc cgcgcgctcc ggcggatagc ccgccttgac gagcgatgga atgaggatcg
    10141 agccgatggc ggacgcgtcc gccaccgagg agcccgagat gcccgagaac agcacctccg
    10201 atgcgaccac cacgatgccc aggccgccgc ggatatggcc gacgatcgcc gaggcgagcg
    10261 ccaccaggcg gcgcgagatg ccgctggttt ccatcagcgc gcccgcgagc acgaacagcg
    10321 gaatcgcgac gaatgggaac ggctcgatgc ccgacatcat cttctgcgcc acggtctcga
    10381 gcgggatgcc gagcgcgatg aagcccgcga tcgtcgcgag cgccatgctg aacgccaccg
    10441 gcatgcccag cacgagcaac gccacgaaca ccgcgacgag gaggatcatg gcttgcgccg
    10501 ctccatcagg acgggccagt ccatcaggac gaaaagcgcg cccgcgatcg gcagcaccag
    10561 gtagccggtg ccgaccgaga acggcagcgt cggatacatc tggaccgccg aactctcgac
    10621 gatgcgccag ccgtacacca ccatgaagac cgcgagcgac cagatcacgg cgtgctcaag
    10681 ccactcgagc agcctcttcc agcgcggccc gaggaagcgg acgaagagat cgacgccgta
    10741 gtgcgcgcga tagcgcaccg cgagcgcggc gccgaggtag gacagccact ggtagcagag
    10801 ggtggtgaac tcctccgacc agacgatcgg ctcctgcacc gcgtagcgga acacgacctg
    10861 cccggcgacc atcaccacga tggtcaccac cagcgccgcc acgacggcgc gcagcgcctg
    10921 ctcgagagcg tggaagaaca acgccttagg gtatgccggc ttccttcagc gccttgtcca
    10981 ccagggggga tttcacccgg ttcttgaacg cctgcaccac cggcatcgtc gccttgcgga
    11041 acgaagcgat gtcgggctcg gtcaccgtca cgcccgcgtt cgccatgaag ccgaccaccc
    11101 ggtcgttggc ggtgttgggg ttgctggtga agtactcgga gtcggtcttg aagaaggcct
    11161 tcgagccttc gtcgagcgcc ttcttctggt cggcgttgag cgagttccac ttcttcaggt
    11221 tcatcaacac cacgcccgag gcgcgcccgt ggttggtgaa cgagaggtgc ttcgccacct
    11281 cgtggttctt cagcgcccag aggagctggg gaccggtcgc gttgccgtcg atctggccct
    11341 gctggatcgc gctgtagacc tcgggcgtgc ccatcggcac cgggatcgcg cccatcgcct
    11401 tgatcatgtc gacctgcatc gggtcctgca tcacgcgcag cttcaggccc ttcatgtcgt
    11461 cgggcgtgcg caccgggcgc ttgctgttca gcaccgcgag cgagccgccg tcggcgaaca
    11521 ccagcacctt gaggccgtgc ggctccatct tcttgccgag ctcggcgacg atcggcccct
    11581 gcaggaacct gatccagtcg tcgtacttca cccacacgaa aggcagcccg aagatgccgt
    11641 gctccggcac gagctgctgg ctaacctgcg aggccaccac ggagatgtcg accgcgccca
    11701 ccttggtcgc ttccatcagc gccttctggt cgcccagcac gcccgccggg aacagctcca
    11761 ccttcagctt gccggtcttg tccaccaccg ccttgaaagc ttccatcgcc gggtagtagg
    11821 cgaaggtctt cggcagcgca tcggcgaact tgagctcggt ctgcgcgacg gcgctgctcg
    11881 cggcgaatag cgccgcggcc gcaacgaatg cgaatttcct catggaatcc tcctccggcc
    11941 gagaatacta cgaaccgccg ccgccgcggt gcgccgattc gaacgactcg atgctgcgct
    12001 cgaggtcttc caggatgggc agatcgccgc gccccccgtg tcgggcaagc ccggtcagca
    12061 gatcggcgat cgacgtcgcc tgcgtgcggt gcttcttctg cagaaacgag aggatgtcct
    12121 gctcgtagtc ctgcacccgc acgctggtcg ctgctgcgcc gcaagagcag ccggcagcga
    12181 acaccacgtg gccgcgggaa actcgctgcc actggccgag caggcgtatc gcttccaccg
    12241 cgggttgttt cgctttcatg cttccagcgc gtaatagtat cgggggcttg accgcccgca
    12301 cccgtcttgc cgccgacatc ggcggtacgt tcaccgatgt cgccgccttc gacgagcgca
    12361 gtggccagct cctgctcggc aagacgctct cgaccccgag ccgcctggtc gacggcatct
    12421 cgcacggcgt ggacaaggcc ggcgcgcgct tcgccgacgc gggcctcttc ctgcacggct
    12481 cgaccatcgc catcaacacc atgctcgagc gcaccggggc gcggaccgcg ctgctcatca
    12541 ccgagggctt ccgcgacacc tacgagatcg ggcgcatcaa ccggccggat tcgtacaacc
    12601 tgtttttccg caagcacgtg ccgctggtcg agcgcgcgct gcgcttcgag gtgcgcgagc
    12661 gcatgacggc gaccggggag gtgctcatcc cgctggacga ggcgcaggtc aacggcctgt
    12721 gcgaccggct cgaggcggcg aaggtcgatg ccgtcgccat cctgctcctg cactgctacg
    12781 tgaatccggc gcacgaggaa cgggtcaagc gcctggtgca gaaaaggctg ccgcgcgcct
    12841 tcgtcaccgc ttcgcacgag ctgtcgcagg agtaccgcga gttcgagcgc tgctcgacca
    12901 cggtcgcgaa cgcctatatc gggccgcgcg tcagccgcta cgtcgccgag atcgatgctc
    12961 acatccagaa ggaagggttc gagggcgcct tcctgctggt gcagtcgacc gggggcctgt
    13021 accagtcgcg ccaggcgcag accgaatgcg tgcgcatgct cgaatcggga ccggcagccg
    13081 gcgtagtggg cgcgcaggcc ctgtgccggg aactgggatt gaaagacgcc gtggccttcg
    13141 acatgggcgg caccaccgcc aaggccggcg tgatctacgg cggcgaagtg ctgaccgcgt
    13201 cgaccgcgct ggtcggaggc tacaacgagg cgctgccgat ccagatcccg atggtgcaca
    13261 tctcggaagt cggcaccggc ggcggcagca tcgccgcggt cgacgccgcc ggtgcgctgc
    13321 gcgtcggccc gcagagcgcc ggcgccgatc ccgggccggc gtgctacgcg ctgggcggca
    13381 cgcgccccac ggtcaccgac gccaacctgg tgctgggacg gctcgccgcc gaccgcttcc
    13441 tcggcggcga gatgaggctc gacgaggccg ccgcgcgccg cgccatcacc gagcacgtcg
    13501 ccaagcccct gggccttggt ctggccgacg ccgccagcgg catcctgcgc atcgcggtgt
    13561 cctccatgtc ctacgcggtc aaaggcgttt cgaccgagcg cggcctggat gccgccgcgt
    13621 tctgcctgat cgcctacggc ggcgccggtc ccctgcacgc ctcgcagatc gcgcgcgaga
    13681 tcggcatgtc gcgcatcatc gtgccgcgcg cgcccggcca cttctgcgcc ttcggcatgc
    13741 tgcactcgga cctgcgctac gacttcgtgc gcacctggtt cacgcgcctc gaggacgcgc
    13801 ccttcggcga gatcgagcgc atctacgagc ggctgatcgc gcaggggaag aaggcgctcg
    13861 ccgagagcgg cgtgcgcttc tcgcgcacca ccgtcgccta cgccgccgac atgcgctacg
    13921 tcggccagga gcatcccgtc accgtcgatc tcgcgcccac ggtgttcaag aaacgcgacc
    13981 gcgacgcgat caagcaccac ttcgacgagg tgcaccaggt ccgctacggc accagcgccc
    14041 ccgaggagcg cgccgagatc gtcagcctgc gcgccaccgt caccggcttg atgaaaaaac
    14101 cgcctctcga gcgaatctcc cgtggaggcg ccgcgccggc gcgtgcggcg caacgcggta
    14161 cgcgaacggt tttcttttcg gacaaaaagc tgaaaacgac tgcctatgcc cgcgccgcct
    14221 tgaaggccgg caaccgaatc tccgggcccg ctctgatcga agagcatgcc tcgacgacgg
    14281 ttttgctgcc gggcgaccgg atgcaggtcg acagctacgg caatctcgtc atcgaggtgg
    14341 gaaggaaaag atgaaacgga aaaaggccaa cgccgatccc atcctgacgg agatcgtgcg
    14401 caacggcgtg ctcgccgtga ccgaggagat gaagtcgaac ctgatgcgca cggcgtacaa
    14461 catgatcatc tacgaggcgc tcgacttcac ggtgggcctt ttcaccgccg acggccgcac
    14521 ggtgtcgatc ggcatgggcc tgccgagctt catccgcggc atggcgaacg tggtgaaggc
    14581 cgagctcgag cacttcgggc ccgagggcct cgagcccggc gacatcctgg tcaccaacga
    14641 tgcctacacc accggcagcc acctcaacca cttcaccttc agcatgccga ttttcgagcg
    14701 cggcaagctg tgcggctggg cggcgtgcat ggcgcactgg cccgacgtcg gcggcaccct
    14761 cggcggcgtc accaccgaca tctactccga gggcatccag atcccgctga tgaagtacca
    14821 gaaggccggg gtggtgaacc aggagctggt cgagatcatc cgcatgaacg tgcgcatccc
    14881 ggagcgcgcg atgggcgacc tgcgcgcgca ggtgatcgcg gtaaagaccg gcgagcgccg
    14941 cttcctcgag ctggtgcgcc gatacggccg cgaggaagtg acggccgcga tcgagaacat
    15001 catgaacaac tccgaagcgg cggcacgggc gaggacgaaa accattcccg acggtaccta
    15061 cgaggccgag tcgttcatgg acgacgacgg ctacaccgtg ggccagcgcg tgccgatcaa
    15121 ggtgcgcgtg gtgaagaaag gcgacgagat gacgatcgac ctttccgaga tcagcccgca
    15181 ggtgcagggg tttttcaact ccgggccgag caccgggcaa tcgtgcgccc aggtcgcgta
    15241 caagtgcctc acctcgccca ccgactaccc ggtgaacgac ggcagcttcc gctcgctcaa
    15301 ggtgatcgtg ccgaagggca cggtggtgag cgcaatcaag ccggccgcca tgcgctggtg
    15361 gatgacgttc ccgatgaccg tggtcgacac ggtgatcaaa gcggtctcaa aagcaatccc
    15421 gcagcgcacc gccgccgcgc accatgccga cctggtgttc gccctgatcc acggcatctc
    15481 gccgcgcgac ggccgcttct acatcgccgc ctgcggcccc gccggcggcg gctggggcgc
    15541 gcggttcaac ggcgacggca tgagcggcgt ggtgtgctcc aacgacggcg acacccacaa
    15601 cagcccctgc gagcagctcg aggccaagta cccgatcctg ttcgagcgct tttgcctgcg
    15661 caccgactcg ggcgggccgg gccggttccg cggcggcctc ggcaccgagc aggtcgtgca
    15721 ggcgctcgcg ccgatcaccg tcgacgtgca ggccgagcgc atgcattgcg cgccgtgggg
    15781 catccacggc ggcatggagg gcaaaggcaa ccacgtcacg acgcgcatcg acggcaagga
    15841 agacaccgac cgcgccaacg ccaagctgcg caaccagcgc ctgaaggcgg gcgacgccct
    15901 catcctgcgc accggcggcg gcggcggctt cggcgacccg cacgagcgcc ccgccgaggt
    15961 ggtggcgaac gacgtgcgcc agggctacgt cagcgccgag gtcgcgcgca gcgcctacgg
    16021 ggttgcgctg aacgcggacg gcggcctcga tacaagggct actgagacgc tgaggaagaa
    16081 taagcgcgtc gcagaatcca tgtgacgtcc gcgtctcaga tcgcgcgcgc ctcaaccacg
    16141 attgcctggg cgtggctgcg gaagggcgcg gcgccgccga gcttcgccag cgccgcagtt
    16201 gcgtgctcga tcacgtcttc caccttcttt ccacgctgcc cgatcagcga tacgcgcggc
    16261 gtgccgcgga tcatgccggt ggcgatcgtg cgggcgtcgg ggcagaccac ctccatgcgc
    16321 cgcggctcga tgcgcacttc cctgaagcgc gcgccggcga gcagctcgcg cagcagcgcg
    16381 ggcgcgtaca tgccgtgggt gatgcgggcg aactgcatct ccgggtctcc ggggaagagc
    16441 tcttcgaaca cctggctgct cgtgagggtg tgcgggtttt cctcgacccg gtcccagacg
    16501 ttgaagagca gcgtgccgcc ttcgcgcagc acgcggcgcg cctcggcgaa cgccgctttc
    16561 ctgtcgggca cgaacatgaa gccgaacgcg cacaccatcg cgccgaactg ggcatcgccg
    16621 aatggcagct tgcaggcatc cgcttcgcgc cactcgatgc cggaggcgcc gcgctgggct
    16681 ttcgcgtatt cgagcatggc cttgctcagg tcgctcgcca ccaggcggac accgggcgcg
    16741 agcttctcgc gcaggtgccg cgtgacgagc ccggtgccac aggcaatctc cagcacgtcg
    16801 ccgccgggcc gggcggcaag gcgggcggcg agatcggcgg cgaactggtc gaaccaggcc
    16861 ggtccgaggc agcggtcgta gtactccgga atcgagccgc tgaagttcgc cgagatatcg
    16921 gtcattgcat gctcttcagc ttgagcatct gcatcgcgtt gccgaaatag atgcgccggc
    16981 ggtcgttcgg cttgagcttc atgccgtcga gcgccttgat ggtctcgcgg atgaagagcg
    17041 gcccgccctg cgggtcgaag gggcagtcgg ttccgaacag gacatgcccc accccgtaga
    17101 aatcgaggcc gcagcggatc gcgctcttcg acccgttcac cgaggtgtcg ccgtagaaca
    17161 tcttgaagaa ctcgaccggc ctttttttca tgcttttcag caacgaaaca taatcttcgt
    17221 ccgaggtcct gctgccgaac tggtccatgc cgtagccgat gcggttgacg aagtacggcg
    17281 ccatcgcgcc gaggtggtgg gtgatgatct tgagcttcgg cagcttttcg aacatgcccg
    17341 agaacaccag gcgcgccatg aatgcgctcg tttcgtaggg ccagccgaac agccaccaga
    17401 tctcgtactt gctcttttct tcggaaaggt agtcgggaaa tttcgccgta cgagtcgggt
    17461 gcacccacac cggcaggtcg taggtcttgc tcatgcgctc gaagatgcga aagaactccg
    17521 ggtcgtcgag cggccggccg ttgacgttgg tgaagatctg gatgcccttg gcgccgagct
    17581 tggcgatcgc ccggtccatc tcggccagcg ccgcgggcac gttgttcatc ggcagcgacg
    17641 ccacgaacgc cgggaacagg tcggggtgct cctggacgat ctcgaacatg ccgtcgttcg
    17701 cgaggcgggc gagcgccggc gtctcctcgg gcgtcccgag gaactcgacc gcgggcatcg
    17761 acagggtgag gatctgcttg tagcccggga agcgcttcat catcttcatg cgccgctcga
    17821 ggtcccacag caccggaatg tgcatccagc gcttgagctg cgccgcgaga cccgggttgc
    17881 cttcggcgat tttcttcatc cgctcgaagt acgcgagggg aaagatgtgc gggaagatgt
    17941 cgagcttgag gttcgtggtc ttcttcatgc tttaaaccat aacatagggc atgtcccact
    18001 ctgtgcgcgg ggccctgtgg atggggggcg cggtgctgtc gttcaccgtg atggcgcttg
    18061 ccgtgcgcgg actgcaggcc cgcatgggct cgttcgagat cgtgtttttc cgcagcgtgg
    18121 tgatgctcgc gatcgtcctc gccatgctgc cgcgggcggg aatggtttcg gttctgaccc
    18181 ggaagttttc tttgcacctg tggcgcaacc tgatccattt cgtcggccag gtgctgtggg
    18241 tgtactcgat cggcgtcctc acgctcgcta ccgtgttcgc gatcgagttc acgatgccgg
    18301 tgtgggcgtc gctgctcgcc tggatgttcc tcaaggagag gctgactttc ccgcggctgg
    18361 tcatgctggc gttcggcctc gcgggcgtgc tggtgatcct gcgtcccggc gccggcagct
    18421 tccacccggc cgcgctggcg atggtgctcg gctcgctgtg ctacgcgtcc tcgttcatct
    18481 tcaccaagcg cctgacctca accgacagca cgctcgcggt gctgttctgg atggcggtga
    18541 tccagacccc gatctcggct gcggcggcgg cgccgcagtg ggtgacgccg ggctgggccg
    18601 acgtgccctg gattctcggc atcggcgccg gcagcttctc cgcccactac tgcatgacgc
    18661 gggcaatgaa gctggtcgat gccatggtcg cggtgccgat cgacttcatc cgcctgccgc
    18721 tgatcgcgct catcggtgcc gcgttctacg gcgaggcgct cgacccgatg gtgttcgtgg
    18781 gcgccgcggt catcttcgcc ggcacttact acagcctctc ccgggagcgg cgcctgccct
    18841 gaccttaata tccataatcc gaacggcatc cccccgcgcg gcgcttttca gccagttggg
    18901 ttcatatgat ggagccgcat ggccgcagag ttcattcccc tcgcccgggc cgcggcgctg
    18961 gtccatgacc ggctcttccc cgggcacggc gtcaaggacg caaaaacgct cgacatgatc
    19021 gcgctggcgc tgtcggcgct gattccgctc taccaggggg acatggagaa aggcgcgctg
    19081 atcgcgctcg aggcgggcga gatcgcgcgc ggccgcttca cccgcggcgc gacccggctc
    19141 gagttccccg accgcgcgcc gctgcgcttc ctgctggtgt cgcgccggga gctttacccg
    19201 gcgatcgacg ccatcgcgaa ggatcctact tgcccgattt ggcggctgtg ccgcccacgg
    19261 ccgccggcgt gttcttctgc gacagccgct tgatcagccc gtcgatgccg tcctgcttga
    19321 ccaccgcgtc gaactcgctg cggtaggtca gcacgaggct cacgccctcg atcacgatgt
    19381 cgtagatctt ccagcccttc tcggtcttgc gcatcgagta gtcgaccagc accggcttcg
    19441 cgccggggcg gatgaactgg ttgtgcacgg tcgcgtcggt gtcgccgggc ttcatgcgca
    19501 ccggcatcac cttcatggtc tggccctcgt aggcgctgat cgcgttcgag taggtccgca
    19561 ccagcatgtt gcggaactcg ccgaccagct tcttcttctg ctccgggctc gcctctttcc
    19621 agccgcggcc gacggcgagg cgcgtggctt cctcgaagtc gacgtgcggc aggatcttct
    19681 cctcggcgag cttgatcgcc ttctgccggt cgccggcggc gagctgcttg tcgctcttga
    19741 tcgtgtcgag cacgtcctgg gtgatcttct tgacgagatc ctcgggaccg gtgtcctgcg
    19801 caaataccgg ggtactcaag aaaaagaaaa ctagcaggaa gcgaaatagg ttcatgcggc
    19861 ggcgcgctcc ttgtggtctt tatccccttt gatattaccc ttgaacatcc ccggctcgag
    19921 ggagtggcgc tcgagcaggc ggtagaactc cgtgcggttg cgccccgcaa ggcgcgctgc
    19981 cttggtgacg ttgccgcccg tgatcttcag gatgcgcacg agatagtcgc gctcgaacgc
    20041 gcgccgcgct tcgtcgagcg gcgtaagcga cgtgtcgccg gcgtccagcg cctgcctgac
    20101 cagagactcg ggaatcatct cggtcgccgc gagggcgacg gcctgctcga tcacgttcag
    20161 gagctggcgc acgttgcccg gccagggggc ggagaccagg atctgcgtcg cttccggaga
    20221 gagcgagagc cggccgcggc gataacgctc tgcgaggcgg gtgaggaaat ggttggcgag
    20281 gagcgggatg tcctcgcgcc gctcggcaag cgtcgggata tagagcttca ccacgttcag
    20341 ccggtagtac aggtcctcgc ggaattcgcc cgaagcgatg cgctcttcga gcttgcgatg
    20401 cgtggccgcg atcacgcgca cgtccacgtc gtactgctcg tgcgagccga cggggcggat
    20461 ttttctctct tccagggccc tcagcaactt gacctggaga ggcaaaggca tgtccccgat
    20521 ctcgtcgagg aacagcgtgc cgccctgcgc cgcctggaac aagccgcggc ggtcggcgac
    20581 ggcgccggtg aaggagcctt tcttgtggcc gaagagctcg gactccagca gcccttccgg
    20641 gatcgcgccg cagttcaccg cgacgaacgg ggcttccgcg cgcgggctcg cccgatgaat
    20701 ggccctcgcc aggagctctt ttccggtgcc gctcgcgccg aagatgcaca cgctcgcatc
    20761 cgaggccgcc acgcggcgcg cctgggacag caggtcttgc atggacgagg agcgcgtgat
    20821 cagctccgcg cgccaggtct cgagctcgcc cgaggcgggc gggctggaaa gccgcatcgc
    20881 ttcggccacg gtgtcgagca gcaccttcgg ctcgaacggc ttggtgagga agctgaagac
    20941 gccgcggcgg gttgcggtca cggcctcggg aatggtgccg tgggcggtga ggatcagcac
    21001 cggcagcgag ggggaatcgc ggtggatggc atcgaacagc gccatcccgt ccatgccttg
    21061 catgcgaagg tcggtgacca ccacctgggg gcgcgacacc gcaagggcgg cgagcgccgc
    21121 ctcacccgat tccaccgcgg tcaccgcgta gccggtggcc gagagccgca tcgcaatgag
    21181 ctgcagcagg tccttgtcgt cgtcgacgag cagcacccgg ggattgtcgt tagccatcac
    21241 cctcacctct tttttccgcg aggccctgat tgttgcggcg catctttacg cagcgtctgc
    21301 aagcgcacgc gccgaacggc gcggcagcgt tacacgaaaa tgaccgcccg cgcccccggc
    21361 caccacggcg atctggccgc cgtgcgcctc gaccagctcg cgcgcgatgg cgaggcccag
    21421 gccgctgccc ttgacccgga cggtggagct gttggccgtg gcgtgctcgg tcctgcggcc
    21481 gcggaagaac aggttgaaga tcgactcgcg ctcctcggcc ggcacgcccg ggccggtgtc
    21541 caggacctcg atgatcgcct ctcccgccac cgcacgcgcc gagaccgaga ccaccccgcc
    21601 cggcggcgtg aacttcaccg cgttcgagat caggttgtcg acgatcgagc gcagcttctc
    21661 cgggtcggcc tcgaccgtcg ccgattccga atcgaccacc agccgcagcc ccttggcgcg
    21721 cgccgcgagc tcgtgcggat gggttgcttc ttccatcacg acggcgagct gcagcggctt
    21781 tacttcgagc gaagcggcgg catgcagcgc gcgctggtag tcgagcagct cctcgatcag
    21841 ccgctgcagc ttgatgctgt tgtcgcgcat gatgccgacg accttgcgct gcggcggcgc
    21901 aagcgggccg gcgacctggt cgttgaggag ctcggcgcct tcgcgcagcg cggtgagcgg
    21961 cgtcttgagc tcgtgcgaca cgtggcgcag gaagcggttc ttctgcgtct cgaactcgtc
    22021 gagccgcctg cgcaaccaat ccagtcttct tcccagataa cgcaagtctt ccggcccgcg
    22081 caccgcgatc ggctgcgaga aatcggcgcc gccgagctgg cggatggcgg cgtcgatctc
    22141 ggtgatcgga cgcgcaatgt agcgggtgag cgcgagcgcg atggcgagcg cgaccgcggt
    22201 gctgaagatg aggagcagga tgatgttccg ctgcaccatc tcggcgctca cccgcaggcg
    22261 ctcgacctcg cggtccacca cgcggttgtt gatcgccagc acgtcgcgcg cgctgtcgat
    22321 cagcgcgtcg atgtgcgcgc cgacctgctg cgcgtccagc cgcggcaggg aggagccggt
    22381 caggaggtcg tacaggccct gctcgcgctc caccgtcacg ttgagcgcct gcgcctgcgc
    22441 gtcgtcgagc ggcagctgcg cgatctcggc ggcgagctgc ttgaagctgc ggtgcacgcg
    22501 cgcgtagtcg gtgacgagct ccgggtcgtt gagcagcgtc ttctgctgcg cgacgcgctc
    22561 gatcgccgcg atgcggtcga cgagcgagcg gctggcgcgc gcggcctgcg aggcgttaaa
    22621 aaccgcggtg cggctcttgc ccgccaggcg ctcggtgttc cacgccgagt acgccagcgc
    22681 gcccaccagc ggcaggatca cgagcgtgaa gcctgcaagc aacagtgcca ggaacgaacg
    22741 cgggtaaaaa atcacgcggt gagttttcca cgacgttgga ccggagccaa tgtcacacgt
    22801 tcacgacgct gcggcgaatc tcgcgtcgct tttccacgac gaccgtctcg tgggagggga
    22861 cgtcctcggt gaccaccgcg ttgggcccga tcgtcgcccc gcgcccgatg cgcaacgggc
    22921 cgatgacttt ggcgcccggg ccgatggtca cgttgtcctc gatctccacg gcgccgagga
    22981 tcgtcacctg gtgcatcagc gtcacccggc tgccgatgcg ggcgccgcgc tcgatgacga
    23041 tgccgaaggg gtagggcatc agcaccggcg agcccagccg gcagcgcagg tcgcagttca
    23101 ggaagagctg caccccgggg atcccgtgcc gcacggcgaa ctgcaagacg cgggccgcga
    23161 gcgggttctc gccgaccagg gcgcgcgggt agcggcgctc gacccggcac tcgagccagc
    23221 gcaggatcgc gcgctcgagc tcgaccacgt tggcggccag gccgcgcccg gcatgcacta
    23281 cgaggtcgtc gcccgtgaac tcgcccggcg gatcgaacac cacgcgcagg tccgcccgcg
    23341 tcgtgccgtc gagaacggcc agcccgagag gcgcgagatc cttcgccacc tgcgccatga
    23401 cctcgccgcg catcgggctt tcgcgcccca cgaaggcgat cgcggcgcgc gccggctcga
    23461 gcacattgcg cgccgccacg gcgagccgcg cgaccaggtc cgccggatcc ggccattgcg
    23521 cgcggcgcag cccgcggcgc agcggcgcga ggctggtgcg caccgcggcc cactcgccgt
    23581 gccgggccgc ctgcgcgacc aggcgggtct gcgcccggtc gggccagaag cgcccgatgc
    23641 gttccagcgc gccgcgcggg tcgtcgttcc agagcgagga cagccacgcc gcgcgctcgg
    23701 cggtgagctc gccgctttcg accgcgtcga ccaggccgtg gctgaagagg gtctcgggcg
    23761 cgccgaccag cagctcctcg gcgcgcaagt agcagcgcgc gccccggcag tagtcgctgc
    23821 agatgcgcac gcgcatgaag cgcggacggc cgacttcgtc gctccacgcg agcacgcagc
    23881 gccacgcccg gctgtcctcc ggcgagagct ggacgagctg gaggtcgagg tcgtggcaga
    23941 agcgtgccac cgcgcgcggc atcccgccga gcgcggccgc ttgcacggcg atctgcactt
    24001 cgggaggcgc gcgctccggg tagccctggc tgtcgccgag gatgcgaaag cgcagcccct
    24061 cgccgctcaa gcggtcgaac agcgcctggg cgacgtagcc gcggtcgtcc atcaaaccgg
    24121 gctgagcacc gtgcccacga cccgcgcctt gcaaagggcg agcaggtccc gcaggcgcgt
    24181 caatgaagcg gcttcgctcc cacgcttcgc gacgacgagg gcaccgccgg cgaaggcggc
    24241 gaagagctgc aggtccgggc cgcgcgccgc cgccggcgtg tggaccagga tcacgcggta
    24301 gcgcgtggcc gccgcggcga gcagctcgag catccgcccg cggctcagga gctcgagcgg
    24361 atcggcgctc gagcgtccgg cgaccagcac ggagaggttc tcggcgcagt gcgcgatctg
    24421 cgtgccgcgg ccttcgagga agtcggcgag cccgtcgcgg ttgcgcaggc cgaaggcgcg
    24481 gtgcagcgcc ggcgcgcgga aatcggcgtc gatcaggagc gtcgcctcgc cgctctgcgc
    24541 gaccgcgcgc gccagctcgg cggcgacgtg cgcgccgccc gccccgtcct gcgggctgac
    24601 cacggcgagg acgcggcgtc cgtgcgcgaa ccagtgcgcg aggagctggc gcgagaggtg
    24661 ctgcggcgcg agcggccgcg cggcgagcag cggctcgccg agcgcatgga tgagctcgcc
    24721 ctcggagcgc accgggcgcg ggcggcggcc gaacaggaac aggctgagaa gcagggccgc
    24781 gatcgcgccg agcgccatgt tgccgccgag gttcggcgcg atggacgtga cgagcggcgg
    24841 gtcgaccagc agcgcctcct tgtggctctc gatgaatccc tgcaccaagg tggcggcggc
    24901 gcgcgggtcc gtgctctcgt agcggacctt catcatcccg gccggcacga gcacgcctcc
    24961 cgtggcgacg tattgccgcg gcgccagggc gctcgccgcc caggcgagcg cgggcccggc
    25021 aaccaatagg aaagcgaccg cacgccagcg tgactgcaac ataggggcga cattacgagc
    25081 aatccgcgtg ccagatctac actgtctgaa aaatcaagga gttcgccaaa acgcaggggt
    25141 gccgattgtc gccgcgtccc gacagtccgt ttgtagctgg ggcgcggcag acagcacaaa
    25201 aaggtcgagc ctagaatcgc gccgactcac gaacaaaagg aagaaacccc ccatgatgaa
    25261 gcgcgggatc ctcgcggcgc tgctcgccct cgggccgaca actgcgttcg ccttcgagac
    25321 ggtcgacacg ctgccttttc catccagcgg cacctttccc ggctggcccg gcgaccgcgg
    25381 cgccgaaccc tggtcgatct atgcctatgg cgggctgatg tacgacaaca atgcctttcg
    25441 ccgcgatacc gacgagatcg acgacatcgt cgcccgctac ggcttcggcg gacgttacgc
    25501 gacgcgcgtc tacggtcgcc agtcggtggt gctcgaagcc ttcggcgagt actacgacta
    25561 caaccatcta agcgagatcg accacttcgg ctatgggctg cgcggcgact ggctgtggga
    25621 gctcggcaac caggccagcg gcaacatcgg gtatgtgcgc cggcagcggc acgcggatct
    25681 cggcgagttc caggtcgagc ggcgtgccat gggcacgacc gacctcgcgt tcgccgacgg
    25741 cggctaccgc ttcctgtccg actggcggat cttcggcgct ctggagtaca cccgctcgtc
    25801 gcgcgagggg gatgagatcg gcgcgctcga aggaaagacg gtgcgcaccg ccctcacgta
    25861 ccgcacgccc ttgggcaacg tcatcggcat cgacgcccgg gccacccgcg gccaggcccc
    25921 gttcacggac gagactacgg gcgctgccac cagcgtgcac gacttcgacg agcgcgtgat
    25981 aggggcgacg ctggcctact ctctcggcac ccagctgcgc atcggcggcc gcgccggcta
    26041 caccactcgc gagtacgacg atctcccgca gcgcgatttc gaaggcaaca cctggcgcgg
    26101 cggcgtcgac tggctgccga cgggcaagct ggttttcagg ttcgagacct accacgagcc
    26161 ggccccgctc accgacctcg actccccctt ttccattcgc accggggcct tgttcagcgc
    26221 aagctgggcg gcaacgtaca agctggtctt cacggcgagc ttcctcaacg agcaccgggt
    26281 gaacgaaagc gacccggtgg ccgcggtgct cggcacgccg gtgcgcgacg agacgatccg
    26341 cgtctggcgc ttcggcgtcg gctgggaggt gcagcgccaa tggcagctcg gagcggcgat
    26401 cgacttcggc gatcgcacct cgaacgaagt cggacgcgac tacgaatacc agcaggtgat
    26461 gctcaacgcc cgcttcacgt tctaggaatt cggggtcagg gtcgcaattc gtttttacgt
    26521 acgtaaaaac tgaattacga ccctgacccc gaattcgctc tgccgtaggc gtcgtcgaac
    26581 cggacgatgt cgtcctcgcc caggtacgcg ccggactgga cttcgatcag ctcgagcggc
    26641 agcttgccgg ggttctccag gcggtgccgc gtgcccggcg gtatgtaggt cgactcgttc
    26701 tcggtgagga ggaaggtctc gtcgccgcgc gtgacgcgcg cggtgccgcg caccacgatc
    26761 cagtgctcgg cgcggtggta gtgcatctgc agcgacagcg cggcgccggg atcgaccacg
    26821 atgcgcttca cctggaagcg ctcgccgttc tcgatgccgt cgtagcttcc ccacgggcgc
    26881 tgcaccttgc ggtgcgccag gcattcggcg cgcccggcgg ctttgaggct cgccaccatc
    26941 gcgccgatcg cctgcgcctt gtccttgcgc gcgaccatca ccgcgtcggc cgtctcgacc
    27001 accacgacgt cgtcgagccc gacgcaggcg agcaggcgat gctgcgagat gaggagcgcg
    27061 ttgcgcgtgt cggcggcgtg cacgtcgccg tggatcacgt tgccgtcggc gtccttcgcc
    27121 tcgagccccc agagcgcgtc ccacgcgccg acgtccgacc agccggcgtc gagccgcacc
    27181 accatcgcgt cgctggtgtt ctccatgacc gcgtagtcga tcgagtcggc gggacaatcg
    27241 gcgaatacgg ccgggtcgac gcgcaggaag ccgccgtcgc gcttgccctg ccggtaggcg
    27301 cgctcgcagg ccctgaggat ctcggggcgc aagttgccga tggcgtcgat ccacaccgag
    27361 gcgcgcaccg cgaagatgcc cgcgttccaa aggtactgcc cggaagcgag gtattgcgcc
    27421 gccttcgccg cgtcgggctt ttccacgaat tcctcgacgt actcggcgtt cgcgcggatg
    27481 tagccatagc ccgtctcggc ggacgcgggc ggcacgccga aggcgacgat ccgccccttg
    27541 cgcgcgagcg ccgcgccgcg ctccaccgcg cgctggaatg cctcgggctg cgcgatgaaa
    27601 tggtccgacg gcatcgcgag cagcaccggg tcgtccgcca tcaccgcgtg caccgcggcg
    27661 agagtgagag cgggcgcggt gccgcgcccc ttcgattcga ggatgatcgc cttcggccgc
    27721 gcgcccgcgc gccgcaactg ctcctggacg aggaagcggt gggtttcgtt gcagacgacg
    27781 agcggctcgc tcgggcagcg cagcgccgtt t
//
LOCUS       DATBSZ010000231        55477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_930188, whole genome shotgun sequence.
ACCESSION   DATBSZ010000231 DATBSZ010000000
VERSION     DATBSZ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 55477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55477
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930188"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="VIG70_14560"
     CDS             complement(<1..1420)
                     /locus_tag="VIG70_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY8251822.1"
                     /translation="MALINGTAGNDNLAGTGGNDTINGLGGNDTLAGSAGTDFYDGGT
                     GFDTLDLRATVTGLTVNLSAGTLSGGFSGTFVNIERVWGSDGGDSLTGTAGADSFSAR
                     AGFDTLAGGSGVDTLWGGTGGDTFIFRETGTANADTIGDWASGSDKLLLDGTVMATLG
                     VSGNFATGDARFKANSTGTATDADDRVLYNTATQQVFYDADGNGAGAKLLIATLQSGA
                     TLVATDIMVEGGSGGGQVINGTPGDDTLTGTSGNDTIDGLGGNDTINGMGGSDSLIGG
                     AGNDLLSDGWNEEDFTEASDHLVGGDGNDTLMSAAGPDFVSSAPDTLDGGLGDDVFHV
                     DNGFDVLIDAGGIDHVIVYDRSWTLAAGFENLTINNDVSESGVTAIGNELDNRIHVTY
                     AASRLDGRGGNDSIVGGGFADTIDGGAGDDTLAGGEFTDDGSDSLIGGAGADSFIIKN
                     AFDDAGAEVIADFASGVDLIRLD"
     gene            complement(1493..2278)
                     /locus_tag="VIG70_14565"
     CDS             complement(1493..2278)
                     /locus_tag="VIG70_14565"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8251823.1"
                     /translation="MSTQAARRLLEAHERRERFTPLPPDLAPRTESEAYAIQDAFVAL
                     RAAKLGALAGYKIALTSAEMRRFVGVESPQAGVMFVSTLHRSPARVRAADYVRLIVEF
                     EIAIQVADDLPAADKPFFRERVAQSVGAVMPALELADDRNADYKALPKHPFELIADNC
                     WNEGAVFGAPVEDWKAIDLAAVRGIATINGNKVGEGRGADVMGHPLDALAWLADHLAS
                     LGRGLLRGDVVITGSIVTSKTVTTGDHVVFDVEGLGKVELRID"
     gene            complement(2247..2762)
                     /locus_tag="VIG70_14570"
     CDS             complement(2247..2762)
                     /locus_tag="VIG70_14570"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HEY8251824.1"
                     /translation="MLAAFNFSRWIDEHAHLLKPPVGNQMVFREAEDLIVQVIGGPNS
                     RSDYHDDPYEEFFYQLRGNMILKVIEEGKPRDIPIREGEILLVPGRRRHSPQRPAGTV
                     GLVVEKVRPRDVDDGFEWYCPKCWALVHRVDVNVQNIVTDLPPLFDAFDRSTRKCPAC
                     GHVHPGSKKTP"
     gene            2786..3157
                     /locus_tag="VIG70_14575"
     CDS             2786..3157
                     /locus_tag="VIG70_14575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY8251825.1"
                     /translation="MKLGQRKYADILVLSPAGRLDHDSCDTFSAELKPFLDDAVAGRR
                     IVLDLSGLEYVSSAGLRLLLLAGKQANSQDAKLVIAAMRPLVAEIFHISRFTMLFEIH
                     AGVREALMAASPQAAAAFDLG"
     gene            3165..4130
                     /locus_tag="VIG70_14580"
     CDS             3165..4130
                     /locus_tag="VIG70_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8251826.1"
                     /translation="MRVRFWGTRGSLPVALTWRDVRDRLARALVVANGRGLDTLEKAQ
                     AFAEHELGFPLTHTYGGHTSCVEVELPQAQPDEYFVCDMGSGARPFGEHVAARQAGRA
                     ATVNVFMSHMHWDHIMGFPFFGPAYRGGNRIRIHGCHAALEQAFRRQQDPPSFPVPFS
                     QLAARIEFVRLTPGEAFELPGGTVTPQLQDHSGDSYGFRFESRGKSLVYTTDSEHKLD
                     DQEARQRIVEFFREADLVVFDSMYSLAEAVSVKADWGHSSNVVGVELCQAARARKLAL
                     FHHEPANGDDAIERILRETRRLEELTRDGHPLEVIAAYDGLEIDL"
     gene            4181..6091
                     /locus_tag="VIG70_14585"
     CDS             4181..6091
                     /locus_tag="VIG70_14585"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEY8251827.1"
                     /translation="MLLLALDLPGVAVWREALFDAYQRILPRSRVSAPAVIVAIDEDA
                     LEARGQWPWPRSLAADLIRAVADAGPAAIGVDLLFVEPDRSAPGADALFAQTLQGRKV
                     VLGIAGLDYRDRRSPLPPKSAPVRRSSGRELPLRSFEGHLQSRAEIDRAAAGRGLLSV
                     DSPDRVIRRVPLIARVGEVVVPALSVEMVRVAAGLPALGIEDRGGQRVALVLGDLAIP
                     VQSDGTLRPYFGRHDPARFVSAEEVLSGMAPRELLRDKLVLIGVTGLGLLDFQATPLG
                     ERIPGVEVHAQILEQIFDGVYLLRPTAAALIEAALLAAAALAFVVLVPRARAATSALL
                     LAGTVLLFVFIGLFAFKAGFVLDVAGPAIGALAVFGALLAATFAEADRQRRALREAQA
                     RVAGEMEAARRIQMGLLPAPRRIFAGDGRFALDAGLEPARVVGGDFYDCFMLDDRRLF
                     FVVADVSGKGLAASLFMALAKSLLKSIALRGGADPAAILTQASAEIARDNTESFFVTA
                     FAGVLDVRSGVLEFCNAGHEPPYLCRPGAAPERLEHPGGPPLTVVEGFAYPCGRRSLS
                     PGETLCVVTDGVTEAMDRAGAFYGAARLRTVLGKQPDVTAVREDVQRFAAGAEQADDL
                     TLLCVRWNGPAS"
     gene            complement(6088..6681)
                     /locus_tag="VIG70_14590"
     CDS             complement(6088..6681)
                     /locus_tag="VIG70_14590"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8251828.1"
                     /translation="MVAVLAIMALVIVFSQPSVPSPAAELVVLVPSADGHTGAVVVEH
                     GGKRTVLDKPYAAHRIGRDSGPAQLTDGQVRSAFAATAAAIPARPAAFLLYFVMGSDE
                     LTEESKGELARMLEELRRRPSPDILVIGHTDRVGNEDDNDRLSLQRAERVRENFVAQG
                     IAAERIRAAGRGEREPVVQTADGVDEPRNRRVEINVR"
     gene            complement(6685..7122)
                     /locus_tag="VIG70_14595"
     CDS             complement(6685..7122)
                     /locus_tag="VIG70_14595"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY8251829.1"
                     /translation="MRWIVTLAALAFAAVSYANDVGQIKNMRGTVHVERDGQKLAAAQ
                     GMSVRQSDVLVTGADGSVGVTFLDNSLLSIGPGSVLAIDRYSFDSTTHDGQFDASLKR
                     GTLAVVSGRMVKRSPESMRVHTPSSIMGVRGTEFFVKVTEPGR"
     gene            7214..7606
                     /locus_tag="VIG70_14600"
     CDS             7214..7606
                     /locus_tag="VIG70_14600"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8251830.1"
                     /translation="MFQARREELHGLRAFVEAFCAQAAMGRDACLRLNLVLEELFTNT
                     LRHGHRGDSDAPVWVSLSGGQDRRVTVSYEDTARPFNPFPRKSPDTTQELHKLEGRGV
                     LLLRTLSVKREYAYLFGRNRIRVVLDTG"
     gene            complement(8182..8268)
                     /locus_tag="VIG70_14605"
     tRNA            complement(8182..8268)
                     /locus_tag="VIG70_14605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8232..8234),aa:Leu,seq:taa)
     gene            8287..9915
                     /locus_tag="VIG70_14610"
     CDS             8287..9915
                     /locus_tag="VIG70_14610"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8251831.1"
                     /translation="MPDVRRLIDEGLLDEAARQLEDAPDTRLMLRLAAALQDRGRPDD
                     ALGWYRRILEVDPGNGDALLCLAVLHEEVDIEQARGFMDRYVALRGDAAGRLRRALML
                     PAIAQSGEHIERVTKRLEAELDELSNGRFAPIRQPEFEVGATPFFLAYYGRNPKPLLE
                     KVARACRAVYPTETRCERKLFASGKKLRIGFISAHFSEHSVAKTMWGFVRDLPRESFD
                     VHVFAIAPAGDAWSERLRRDADHYAALPVDLERARDAIASAKLDIAFFTDIGMDPLTY
                     FLAFWRLAPIQLNSWGHSVTSGIDTIDYYVSHDGVELPEAQSHYSEKLIRLPGYCMPR
                     YLRPALAGAKKTREALGLPPARRLYYCPQNLFKFHPDFDAALKAILERDSEGEVVLVD
                     SARPWTGQLAQRLSRTLGAVGARVRLLPRMRHADFLQHLSAADVVLDPFYFGGCNSSC
                     DTFALGAPIVTLPGFLLPGRFTLGLYKEMNIEECVARSPDEFVDIALRLGHDPAYREE
                     ISRRISRNAERLFDRPDCGRALGAALLRIAEERR"
     gene            complement(9915..9985)
                     /locus_tag="VIG70_14615"
     tRNA            complement(9915..9985)
                     /locus_tag="VIG70_14615"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9951..9953),aa:Cys,seq:gca)
     gene            complement(10029..10104)
                     /locus_tag="VIG70_14620"
     tRNA            complement(10029..10104)
                     /locus_tag="VIG70_14620"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10069..10071),aa:Gly,seq:gcc)
     gene            complement(10127..10690)
                     /gene="pgsA"
                     /locus_tag="VIG70_14625"
     CDS             complement(10127..10690)
                     /gene="pgsA"
                     /locus_tag="VIG70_14625"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY8251832.1"
                     /translation="MPLNAPNLVTLSRIILIPLLIGIYYLPWFSAETMNLTATAIFIF
                     AGITDWLDGYLARRLNQMSAFGAFLDPVADKLIVVAALLALLYLNRVDMIVALIIVGR
                     EIAISALREWMAKVGQAKSVAVAFIGKLKTVAQMVAIPLLLFHDVLLGLDCQWLGTVL
                     INIAAVLTVISMLYYLRKALPYASVRG"
     gene            complement(10690..12468)
                     /gene="uvrC"
                     /locus_tag="VIG70_14630"
     CDS             complement(10690..12468)
                     /gene="uvrC"
                     /locus_tag="VIG70_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY8251833.1"
                     /translation="MFDAKSFVAGLPNLPGVYRMLGRGGEALYVGKARDLRKRVASYF
                     NKQAHGPRTQMMIGQVASIEVTATRSEGEALLLENNLIKSLAPRYNILFRDDKSYPYL
                     TITGHEFPRLGFHRGAKDKRHRYFGPFPHAYAVRESIQLLQRVFRLRTCEDTVFEHRS
                     RPCLLHQIKRCTAPCTARITAALYAEDVANAALFLEGREDDVVGNLNRKMNAASEARR
                     YEEAAAYRDQVRALSRVQARQYVESNRGVDADVVACVIDGEIACVNLVMIRTGRHVGD
                     RSFFPANAEGAAAAEVIAAFLEQHYAEQPAPGLVVTSEAVEIEGIHVINPSHGERKVW
                     LDMARKNALLAIAQRVRDRATQEGRLLALREALGLPEGAQRVECFDISHTMGEATVAS
                     CVVYDRSQMQKAEYRRFNIRDVTPGDDYAAMRQALERRYERVTAEAGKIPDLILIDGG
                     KGQAKIASAVLHDLGLHQACVVGVAKGPGRKPGEEELILESEARTLQLAPAHPGLHLI
                     QAIRDEAHRFAVVGHRARRGKARTTSMLNEIPGIGAKRRQALIEHFGGLRGVQAAAVD
                     DIAKVDGISRPLAERIYRHLHESAAA"
     gene            12521..13087
                     /gene="efp"
                     /locus_tag="VIG70_14635"
     CDS             12521..13087
                     /gene="efp"
                     /locus_tag="VIG70_14635"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY8251834.1"
                     /translation="MAKVEAVGIRPGNLLEWDKRVWRVLKSYHVHVGGRGGAFMQVEM
                     KDIETGTKTNQRFRTEDKVERAFVDPRDMQYLYQDGDSYVFMDKENYEQLTLPAEFLE
                     GQAGYLLPNTDVQINFYNSRPIGIDLPPSVVLTVADTEPGIKNSTATNSFKPATMETG
                     ITVQVPPFINKGEKIKIDTAEGTYMERA"
     gene            complement(13092..14114)
                     /gene="nagZ"
                     /locus_tag="VIG70_14640"
     CDS             complement(13092..14114)
                     /gene="nagZ"
                     /locus_tag="VIG70_14640"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY8251835.1"
                     /translation="MRKVRRPLGPAVIDVVGTQVSDEDRERLRHPAAGAVILFSRNYE
                     NTQQLSMLVEDIEKLREPALLVCVDHEGGRVQRFREGFTAIPAMRELGRLWDRDKEAA
                     KEAARGAAYVIAAEVGAHGIDFSFAPVLDLDYGSSSVIGTRALHYDPNAVGALAAAIV
                     QGFASAGMAAVGKHFPGHGFAAADSHVDVPKDERAFSEILKKDVAPYRAAFQAGLAGV
                     MPAHVIYTQCDPEPAGYSKYWLQEVLRAKLGFDGLIFSDDLSMAGASTVGGPPERARA
                     ALAAGCDMVLLCNDPEGQNKLLESLGELQMKNPERLERMRKKGGRDLRKSVAYREAQE
                     ALTALA"
     gene            complement(14102..14485)
                     /gene="acpS"
                     /locus_tag="VIG70_14645"
     CDS             complement(14102..14485)
                     /gene="acpS"
                     /locus_tag="VIG70_14645"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY8251836.1"
                     /translation="MIFGVGTDVVEIGRIEKALGRWGERFARRILCEPELKRFLGHRQ
                     PASYLAKRFAAKEAFTKALGTGIKAPANWHGVWVMNRPSGKPELEFSKQLQKLLSGRG
                     ISRAHVSLADERGVAVATVILECEK"
     gene            complement(14482..15228)
                     /locus_tag="VIG70_14650"
     CDS             complement(14482..15228)
                     /locus_tag="VIG70_14650"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014111517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY8251837.1"
                     /translation="MIELGVNVDHVATVRQARRTYEPDPVWAAVEAHLGGADGITVHL
                     REDRRHIQDEDVRRLRELTHIKLNLEMAATDEMVGIAGRIKPEMAMLVPEGRHEVTTE
                     GGLDVVSQEKSLKRAVQQLSAAGIVVSVFIDAELPQVEAAKRIGASVCEVHTGPYAHA
                     FHSKGRDAESPAVVSELKKIQAAGDAIRSLGMRFNAGHALNYFNVQPVARLPGVRELH
                     IGHAIVSRAVFVGIREAVREMKSLMRQAAG"
     gene            complement(15245..15961)
                     /gene="recO"
                     /locus_tag="VIG70_14655"
     CDS             complement(15245..15961)
                     /gene="recO"
                     /locus_tag="VIG70_14655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY8251838.1"
                     /translation="MKRRADHQPGYVLHTYPYKETSLIVEAFTRGFGRVALLARGARR
                     PRSAMRGVLLSFHPLRLGWSASAELGNLISAEWSGALQPLAGRGLMCGFYLNELLLRL
                     LPRDDAHEALFDFYGEALAGLSAGATHASVLRSFEKRMLAELGYAPLLEREAASGAPI
                     EPGRRYVYEPDKGPVPSRGSGNGDLVVSGQTLLDLAADEYARPETREEARMLLRALIG
                     QRLHGQVLHTRAVLRELTDL"
     gene            complement(15958..16842)
                     /gene="era"
                     /locus_tag="VIG70_14660"
     CDS             complement(15958..16842)
                     /gene="era"
                     /locus_tag="VIG70_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011351415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY8251839.1"
                     /translation="MSAYRCGTIGIAGRPNTGKSSLLNRIVGEKLSIVSSKPQTTRHL
                     VTGILTRPDCQYVFVDAPGQQAQAKNTLHRRLNRRASQAARDADVALFVVEALRFGPD
                     DQMTLDKIPAEQRVVAAVNKIDRLKNPEQLIPFLDRLSKTRDFAAIVPLSAKTGKNLP
                     ELLAALRSALPEAPAAYPPDQLTDRDERFFAAELLREKLFEAMGEDMPYRCEVVIDSF
                     KEEGRLRRIEATILVERDSQKPIVLGAGGERLKRMASAARKDMEKLFGGKVYLGTWVK
                     VRKAWTDDARVLRQLGYE"
     gene            complement(16839..17453)
                     /gene="rnc"
                     /locus_tag="VIG70_14665"
     CDS             complement(16839..17453)
                     /gene="rnc"
                     /locus_tag="VIG70_14665"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY8251840.1"
                     /translation="MLGYRFTDAQLLERALTHRSHGPDHNERLEFLGDGVLGCAVADE
                     LYARFPQLPEGKLTQMRASLVREESLVEVAKALGVAARLRAGSVQDSMLADSLEAIFG
                     AIFLDGGYPAARRAVVQAFGPLLETLDPGKVDKDAKTRLQELMHARGKPLPEYRVVAT
                     HGAPHQRSFEVECVVEGLQARGEGKSRQRAEQEAARAILNKLEL"
     gene            complement(17490..17840)
                     /locus_tag="VIG70_14670"
     CDS             complement(17490..17840)
                     /locus_tag="VIG70_14670"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HEY8251841.1"
                     /translation="MQKGQHGVTITGLLVVLIIAGMLALLAMKVIPSFLEFRTAKTAI
                     ELIARSSQNPAEVRRAFENRAAIDNIQTIRPQDLEITRDGNQMVIAFAYRKEVPLFGA
                     VGLYIDYAANSRGN"
     gene            complement(17861..18649)
                     /gene="lepB"
                     /locus_tag="VIG70_14675"
     CDS             complement(17861..18649)
                     /gene="lepB"
                     /locus_tag="VIG70_14675"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY8251842.1"
                     /translation="MNFALFLLILLVVTGGVWAMEMLWLRRSRAQHAKQPWWVEYSVS
                     FFPVILIVFLLRSFLIEPFKIPSSSMVPTLLVGDFILVNKYTYGIRLPVANVKVVELG
                     SPERGDVMVFRFPEDPSLDYIKRVVAIPGDRLEYRNKRLSINGTPVPTRQVDDYLSKE
                     RMQFSRRYVETVNGVEHEILLDEDSPPSMMPGRAFPFAGNCNYNTNGLACTVPPGHYF
                     VMGDNRDNSSDSRVWGFVPDANIVGKAFFIWLNLNELGRFGSFR"
     gene            complement(18678..20477)
                     /gene="lepA"
                     /locus_tag="VIG70_14680"
     CDS             complement(18678..20477)
                     /gene="lepA"
                     /locus_tag="VIG70_14680"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY8251843.1"
                     /translation="MAQSRIRNFSIIAHIDHGKSTLADRFIQMCGGLAEREMAEQVLD
                     TMELERERGITIKAQTAALEYRAKDGETYLLNLIDTPGHVDFSYEVSRSLAACEGAVL
                     VVDASQGVEAQTVANCYTATEQGVEVIPVLNKIDLPSADPEKAIQEIEDVIGIQAQDA
                     IRASAKTGENVDAILEAVIARVPAPAGDPANALQALIIDSWFDNYVGVVMLVRVMEGT
                     IRPKEKILLMSTGGSYLCDQVGVFTPKSKFKEDLKAGEVGFVTAGVKELRAAKVGDTI
                     TTAVKPAARPLPGFKEIKPQVFAGLYPVEANEYESLREALEKLHLNDASFHYEPETSQ
                     ALGFGFRCGFLGLLHMDIVQERLEREYGQSLITTAPSVVYEVKLRDGTLEQLSNPSRL
                     PPPETIEEFREPIMACKIFVPQEYVGPVITLCTDKRGTQTNMHYSARHVVISYDLPLN
                     EVVLDFFDRLKSMTRGYGSLDYEFKEYRAADMVRLDILVNGERVDALSSMAHRSGAQQ
                     RGRELVVKLRSLIPRQMFDVAVQAAIGGHIIARENVKALRKNVLAKCYGGDVTRKRKL
                     LEKQKEGKKRMKQVGAVEIPQEAFLAVLQRGEG"
     gene            complement(20494..20694)
                     /locus_tag="VIG70_14685"
     CDS             complement(20494..20694)
                     /locus_tag="VIG70_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY8251844.1"
                     /translation="MYSRSGCHLCEEMAAGLRALGIAFDEIDVDTDPHLRLQYGRDLP
                     VLVYKDVVLKHRLTPEAIAKLR"
     gene            complement(20696..22147)
                     /locus_tag="VIG70_14690"
     CDS             complement(20696..22147)
                     /locus_tag="VIG70_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY8251845.1"
                     /translation="MNAVRAVVTLWLAVFCAAAPAPALSQAVRELPDFTRLVDDQGNA
                     VVNISTTQAVRRSGAAPQVPGIEDEEVLEFFRRFIPRQQPGPGQGPRQPEARSLGSGF
                     IISADGYILTNAHVVEGADEITVRFTDKREFKAKVIGADRRTDIAVIKIEAASLPAVK
                     FGDPTKLKVGEWVVAIGSPFGFENTVTAGIVSAKGRSLPQENFVPFIQTDVAINPGNS
                     GGPLFNMRGEVVGINSQIYSRTGGFMGLSFAIPIDVALDIQKQLREKGRVSRGRIGVV
                     IQEVTRDLATSFGLDRPRGALVNSVEKGSPADKAGIEATDIILSFDSKQVESSSDLPR
                     IVGSTRPGSQSALEVWRRGKTQKLNITVAELQEDRVASSTTPGRSKPQAEAPANRLGI
                     VVGELSAEQRKGLGVTGGLVVTDVRPDSKADVRKGDVLLTMVHKGQHTELKTVEQFNK
                     LLAGLDKNAVITLQVRRGESTAFVTISGLTDKG"
     gene            complement(22164..23108)
                     /locus_tag="VIG70_14695"
     CDS             complement(22164..23108)
                     /locus_tag="VIG70_14695"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEY8251846.1"
                     /translation="MLRAFLIAVALGSGAAQAQSPETLGWLRKMHDATQKLSYTGTFV
                     YQNGPRSETSRITRYVDASGDIEKLEVLDGMPREIVRTKDTVRCYLPGLRLVKVDRRT
                     ERDFPALLPERITALARHYDISLGETRRIANFDCQAVVLTPRDNLRYGYRLYADTNSG
                     MLLRAVTVDAAGEQVEQFTFTQLTIGGVSREMVKSRHASRSWRTEDTEAAPARLAGWG
                     LSSELPGFQKIVELKRRLGESRTAGQVVYSDGLAAVSVFIESLEGRSDPVRTGLSSMG
                     AIHIYTREVANHMVTVVGEAPAASVQRIANAVEYRRPQ"
     gene            complement(23108..23614)
                     /locus_tag="VIG70_14700"
     CDS             complement(23108..23614)
                     /locus_tag="VIG70_14700"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HEY8251847.1"
                     /translation="MKERLSELMDGELDDRSAAAVLEALGRDREARQAWGTYHMIGDS
                     MRGGRPLSGGFAARLSARLAAEPTVLAPRALPAEPRRWFALSAAASLAAVALVGWMAF
                     APQPQVAPGPTPVAQAQPEAKPNIVPLPTAANDYLLAHQGFSPRVSLQGMVPYVRTVS
                     EQAVEPRR"
     gene            complement(23619..24218)
                     /gene="rpoE"
                     /locus_tag="VIG70_14705"
     CDS             complement(23619..24218)
                     /gene="rpoE"
                     /locus_tag="VIG70_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEY8251848.1"
                     /translation="MTDREIDRQLVARAQGGDKQAFELLVEKYQRKLARLLSRFIRDP
                     AEVEDVTQEAFIKAYRALPAFRGDSAFYTWLYRIGINTAKNYLMAMGRRAPTSTEVEA
                     EEAEGFEEGEQLRDINTPESVLLSNEIAETVNSTIEGLPEELRRAIQMREIEGMSYED
                     IAQAMNCPIGTVRSRIFRAREAIAEQLRPLLDTRKDQRW"
     gene            complement(24371..24808)
                     /locus_tag="VIG70_14710"
     CDS             complement(24371..24808)
                     /locus_tag="VIG70_14710"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HEY8251849.1"
                     /translation="MSGTRLELSRSWGLAGAILALHAAAAASVITVMGTALGYALGAA
                     FLALGMAAAWARALHGSKASVRALELSRNEILLELKDGRRVQAELGPARHVSRMMVTL
                     PLRRPMRRTILVARDMLGAEEFRRLRLWALWGRLPVAAKQLPA"
     gene            complement(24805..26043)
                     /gene="fabF"
                     /locus_tag="VIG70_14715"
     CDS             complement(24805..26043)
                     /gene="fabF"
                     /locus_tag="VIG70_14715"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY8251850.1"
                     /translation="MNRRRVVVTGLGIVSPIGNTVADAWASALAGKSGITRITRFDPS
                     RLASQIAGEVKGFDVAPYMSPKEARRMDVFIHYGMAAGLQAWREAGLQVTPQNAERIG
                     INFGSGIGGLPLIEAQHHELEQKGPRRLSPFFIPGSIINMTAGLLSIEIGAKGPNVAM
                     VTACTTSTHCIGEAMKSIRYGEADTMIAGGSEAVVTELAVGGFASARALSTRNDDPTT
                     ASRPWDKERDGFVLGEGAGAVVLEEYEQAKARGAHIYCELIGYGLSADAYHMTAPSED
                     GDGAFRCMRNAMKDAAIEPSAIDYINAHGTSTPLGDIAETKAVKRVLGAHAGSVAVNS
                     TKSMTGHLLGAAGGIEAVFTALAIRDQVSPPTINLRTPDPECDLDYVPNVARKMPIRV
                     ALSNSFGFGGTNATLVMARP"
     gene            complement(26044..26283)
                     /gene="acpP"
                     /locus_tag="VIG70_14720"
     CDS             complement(26044..26283)
                     /gene="acpP"
                     /locus_tag="VIG70_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY8251851.1"
                     /translation="MENVEQRVRKIIAEQLGVNEGEIKNDSSFVDDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKITTVQQAVDYIKAHAKS"
     gene            complement(26314..27033)
                     /gene="fabG"
                     /locus_tag="VIG70_14725"
     CDS             complement(26314..27033)
                     /gene="fabG"
                     /locus_tag="VIG70_14725"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY8251852.1"
                     /translation="MLEGKIALVTGASRGIGHAIAEELGRQGARVIGTSTTAEGAKKI
                     NAPINGRVLNVRDAAQCDALVESIQKEVGDIAILVNNAGITRDNLALRMKDADWDEVI
                     ETNLRAVWLLSRKVMRAMIKARWGRIINITSVVGAAGNAGQANYAAAKAGVVGMTKSL
                     ARELGSRNITVNCVAPGFIDTDMTRALSDEQRKVLLENIPLGRLGSAQDVAAAVAYLA
                     SPGGGYVTGAVLHVNGGMYMS"
     gene            complement(27027..27944)
                     /gene="fabD"
                     /locus_tag="VIG70_14730"
     CDS             complement(27027..27944)
                     /gene="fabD"
                     /locus_tag="VIG70_14730"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY8251853.1"
                     /translation="MKLAMVFPGQGSQAVGMLKAYAGLPEIEGVVAEARTALGEDFVK
                     LLDEGPAEKLTLTVNTQPAMVTAGYAAYRAWRGLGGPQPEFVAGHSLGEYTALVAAGA
                     LSFKDCLPLVRFRAQSMQEAVPEGQGAMAAILMLDDDAVRAACAQAGGAVQAVNFNAP
                     GQVVIAGEKAAVERAIEICKAKGAKRAMLLPVSAPFHSTLMRPAADKLKTYLEKAALT
                     APKIAVVNNVDVAVENEPGKIRQALVRQAASPVRWVETIRRMADSGVTHIVECGPGKV
                     LSGMTKRIAPGVKSLAAADRASLEEALKC"
     gene            complement(27941..28900)
                     /locus_tag="VIG70_14735"
     CDS             complement(27941..28900)
                     /locus_tag="VIG70_14735"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY8251854.1"
                     /translation="MIYSRIAGTGSYLPPRVMTNAEFAARLDTSDAWIRERTGIVQRH
                     IADKSQASSDLALEASRRALESSGVSASEIDLIVVATSTPDYVFPSTACLLQAKLGVK
                     GSAAFDVQAVCSGFVYGLATADSFIKSGQYRKALVVGAEVFSRILDWNDRGTCVLFGD
                     GAGAVVLVSSENPGIHASVLRADGSQAGMLSVPGNVCGGTVIGSPFLQMNGQAVFKFA
                     VKVLDESARETVAAAGLKLADVDWLIPHQANVRILDATARKLGLPPAKLVVTVDHHGN
                     TSAASVPLALDEFVRAGKIRPGHRVLLQGVGGGFTWGSSLVTM"
     gene            complement(28897..29904)
                     /gene="plsX"
                     /locus_tag="VIG70_14740"
     CDS             complement(28897..29904)
                     /gene="plsX"
                     /locus_tag="VIG70_14740"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY8251855.1"
                     /translation="MITVAVDCMGGDHGAHVTVPAALEFQARTPDVEVVLVGQRDAIE
                     SHLSGTNPRVRVYPASEVVAMDEPPAQALRYKKDSSMRVAVNLVKSGEAHACVSAGNT
                     GALMAISRFVLKTLPAIDRPAIATVLPNQRRTSTYVLDLGANVDCGAEHLLQFAIMGA
                     MLVAAVEHKERPSVGLLNIGVEDIKGNEAVKQAAVLLRGSGLNFVGNVEGDDIYKGTV
                     DVVVCDGFVGNVALKASEGVAQMMTTTMREEFSRNPLTKLAALVAMPVIRAFRERLDP
                     RRYNGASLLGLRGIVIKSHGSADVFAFGQALERAVEEVRNNVPQRIAERMSQLQPQPV
                     Q"
     gene            complement(29910..30089)
                     /gene="rpmF"
                     /locus_tag="VIG70_14745"
     CDS             complement(29910..30089)
                     /gene="rpmF"
                     /locus_tag="VIG70_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY8251856.1"
                     /translation="MAVHQNKKSPSRRNMRRSHDHLSAPAIAVEPTSGETHLRHHVSP
                     TGFYRGKKVVKTQEE"
     gene            complement(30109..30372)
                     /locus_tag="VIG70_14750"
     CDS             complement(30109..30372)
                     /locus_tag="VIG70_14750"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251857.1"
                     /translation="MALEVHTDETLVLAATLAEIHAEPADARAPDRVVAGKEMALREL
                     IEDELILAVPYAPRHEDCAPAAASDGAQKTSPFAGLRGLISKH"
     gene            30661..31242
                     /locus_tag="VIG70_14755"
     CDS             30661..31242
                     /locus_tag="VIG70_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEY8251858.1"
                     /translation="MPQPRRLVLASGSRYRKALLERLGLPFEAASPDIDETPLSGESP
                     PDTALRLAHLKAMALSHRGDLIIGSDQVAASGGRRYGKPGGHEKAVRQLRELSGKAVD
                     FHTAVTLLDTRRDKHESRLVPCRVVFRTLDERRIQAYLEREKPYDCSASAKAEGLGIA
                     LIERIETDDPSSLIGLPLIALSEMLERAGLPVL"
     gene            31332..31955
                     /locus_tag="VIG70_14760"
     CDS             31332..31955
                     /locus_tag="VIG70_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8251859.1"
                     /translation="MPQDVVRQVQALRDFVVENAKSARAFLGALGMPVRELDIRVIGE
                     ERDPLAPLREKRSLGLLSEAGCPAIADPGAAIVEAAHREGFRVVPLVGPSSIVLALMA
                     SGLEGQRFAFCGYLPRDDSQRKQRIRELEQRSRKEQETEIFIETPYRNDVLLAALVET
                     CNTGTRLCVAADLTLPSESVQTKPIGEWRRAKAAIGRRPAVFLLLAD"
     gene            complement(31944..32882)
                     /locus_tag="VIG70_14765"
     CDS             complement(31944..32882)
                     /locus_tag="VIG70_14765"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEY8251860.1"
                     /translation="MTENGGQWERELVNKLATAALKEQRRARLWGIFFKLLTFAYITV
                     FLLMAIDWKGGDVGGSKKHTAMVEVSGLIAPGTDASAEKITTALQAAFKDKNTQGVIV
                     RINSPGGSPVQAQTIYDEMKRLRSKYPEIPLYAVVEDVCASGGYFVAVGADRIYVGKA
                     SIVGSIGVLMNGFGFTGLMDKLGIERRLITAGENKGMLDPFSPLDDKDKQHIKTLMGD
                     IHQQFIGVVREGRGKRLKEGPEIFSGLIWTGQKSVDLGLADGFGSLEYVAREVIKAEE
                     IRDFTISEGVAEKLARRFGASVSSAFVEALLRTSIR"
     gene            complement(32875..33231)
                     /locus_tag="VIG70_14770"
     CDS             complement(32875..33231)
                     /locus_tag="VIG70_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8251861.1"
                     /translation="MAASERLICESGVLIDAGRGVRFEVEYFGEAAPAFAVRKDGKVH
                     AYLNRCAHVAMELDWQEGVFFDSDGRDLLCSTHGATYDAATGRCLGGPCNGSPLVKLR
                     LEERNGLVYFIGFDDD"
     gene            complement(33210..33857)
                     /locus_tag="VIG70_14775"
     CDS             complement(33210..33857)
                     /locus_tag="VIG70_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY8251862.1"
                     /translation="MSYRLLVFDWDGTIIDSAATIADCIRDASRELGLEVPDWQRASH
                     VIGLGLHDAMRIAVPGLAAERYPEFVASYRKHFLVRKDAMQLFDGMRPLLEDLSKRHV
                     LAIATGKSRRGLDRDLEFHRLKPLFAASRCADETNPKPHPAMLREIIEELAVPKDSSL
                     MIGDTSHDLEMARAAGVDALAVTYGAHPEDSLRACEPLGCFSSVTQLEEWLRANA"
     gene            complement(33854..34780)
                     /locus_tag="VIG70_14780"
     CDS             complement(33854..34780)
                     /locus_tag="VIG70_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY8251863.1"
                     /translation="MNDLSKTAGPKAKWLVIGEESEAQRIDNFLLRQLKGVPKSHVYR
                     VLRSGQVRVNSGRVKPDYRLQVGDRVRIPPVRVSEGRPPPARPLDLPVVFEDASLLVI
                     DKPSGIAVHGGSGVSFGVIESLRAARPQAKVLELAHRLDRDTSGLLILCKRRSALVEL
                     HRMLREGEVRKIYLAIVKGALAKKTLELRESLHKHVTASGERRVSVQADGMSALTRVT
                     RVKSSGEFSLLQVELMTGRTHQIRVHLAHAGHPVVGDDKYGDFALNRALKQRLLLHAA
                     KLSFRHPLGGEPVKLESPLPAEMKAFLEKMLP"
     gene            35040..37763
                     /locus_tag="VIG70_14785"
     CDS             35040..37763
                     /locus_tag="VIG70_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY8251864.1"
                     /translation="MKRMLFNATQQEELRVAIVDGQKLVDLDIESASREQRKSNIYKG
                     IITRIEPSLEAAFVDYGEERHGFLPFKEVSRSYFKDGIDAGRARIQDALREGQELIVQ
                     VEKDERGTKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGEDRNELRDTLDQLQLP
                     EGMSVIARTAAIGRSLEELTWDLNYLMQLWTAVEGASADQSKKPFLIYLESSLVIRAI
                     RDYFQPDIGEILIDTEEIFEQAKSFMQTVMPDNVGKVKLYRDDIPLFSRFQIEHQIES
                     AYSRGVALPAGGAIVIDHTEALVAIDVNSARATRGGDIEETAFRTNLEAAEEVARQLR
                     LRDLGGLIVIDFIDMESQKNQREVENRLRDSLRYDRARVQIGKISRFGLMELSRQRLQ
                     ASLEETAHISCPRCSGTGFIRGTESTALHVLRIIQEESMKENTGAVHAQVPVDVSSFL
                     LNEKRAEIQKLEARLKVNIVLVPNPHLETPHYKVQRLKHDELNAMEHVPSSYELVERP
                     EEPKAPGAEEEVKLERQQPVVKTITPDQPAPLPQAAAPQPAPRPAQPASQPAPMPAAP
                     QPQPASGGGFLSRLFGGLFGAEPAPAPTPVAASPAPQASQPAQREPRRDHHRRDGRRD
                     GGRGRDGQRDGRRDEQQRDRQQHPRLEQRRNGQQPRGDRGERDRGGRGERGRPDHQQR
                     QQRPQHQGPQQQGQQPQQQEQQPPPQEQQPQPQGQQPQQQHRPPPREREAREGREGEE
                     QQRRGGRRNRGGRDRQRERGDRGERPAQGDPRPSGAPPSFLMKEDQLREDQRREALAR
                     KELESSGEETLPPSVAPRVEEPAPRMEQAAPRMEQPAPHAEQPVRAEPKIDSKVLLES
                     AGLVMIETDRARVPAAAPAQEEQQHLGRPRRERQKPVVQDEPLQQVETTRK"
     gene            complement(37753..38211)
                     /locus_tag="VIG70_14790"
     CDS             complement(37753..38211)
                     /locus_tag="VIG70_14790"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEY8251865.1"
                     /translation="MKERRSILFVCTGNICRSPTAEGVFRSLCVKAGLELRIESAGLG
                     DWHVGHPPDERAQHHARSRGYDLSAQRARQVRPRDFDDFDLILAMDSGHLRDLQRMAP
                     PRHRANIRLFAADSDVPDPYYGGPAGFERVLDLVEAQCASLLAELKEPTS"
     gene            38224..39615
                     /gene="asnS"
                     /locus_tag="VIG70_14795"
     CDS             38224..39615
                     /gene="asnS"
                     /locus_tag="VIG70_14795"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEY8251866.1"
                     /translation="MTSCADVLAGRAPKEGAVVLKGWVRTRRDSKAGFSFIHLSDGSS
                     FHPVQVVAPKDLPNYEKEILHLTAGCAIEATGGIVPSPAKGQPFEMQARAIKVVGWVE
                     DPDSYPIQPKPHTFEFLREVAHLRPRTNVIAAVTRVRHTLAQAIHRFFHERGFFWVNT
                     PIITAADAEGAGALFRVSTLDFANLPREGGKIDFSKDFFGRETFLTVSGQLNVEAYCL
                     ALSKVYTFGPTFRAENSNTSRHLAEFWMIEPEIAFADLSDNADLAEALLKSIFKSLLQ
                     ERKEDMAFFDERVEKGIVAKLQGIVESEFVRMDYGEAISILERAKEKFEFPVKWGVDL
                     QSEHERYLTEKHAKKPVVVMNYPKAIKAFYMRLNDDDRTVAAMDVLAPGIGEIVGGSQ
                     REERLDRLDARMAEVGIDKESLSWYRDLRRYGTVPHAGFGLGFERTVSYATGLSNVRD
                     AIPFPRTPGNAKY"
     gene            39615..40082
                     /locus_tag="VIG70_14800"
     CDS             39615..40082
                     /locus_tag="VIG70_14800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY8251867.1"
                     /translation="MRIRLATPADAAVLIEFNSAMALETERKDLLAEVISAGVHGLLG
                     SAAAGFYVVAEKDDRVVGSLLITKEWSDWRNGSFWWIQSVYVRPEWRRQGVYKQLYRH
                     VQELAAQDPAACGFRLYVERENSRAQATYRALGMKETRYLVFEELKPGVRFYR"
     gene            complement(40073..40621)
                     /locus_tag="VIG70_14805"
     CDS             complement(40073..40621)
                     /locus_tag="VIG70_14805"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEY8251868.1"
                     /translation="MFLKLRLMWWRADKAALAFAMFFCAVVGVLVLALGAVSAHRDEQ
                     TARVREFHARSLECLALNVYYEARGEPPAGQYAVAEVTMNRKASPLYPKTVCEVVYQT
                     NWDPLRKRYVGAFSWTEFESLDAPKGTAWKRAVKVAEDVYYQRRQAVMPGALHFHANY
                     IQPEWAKERQKVARIGRHVFYR"
     gene            complement(40765..42753)
                     /gene="uvrB"
                     /locus_tag="VIG70_14810"
     CDS             complement(40765..42753)
                     /gene="uvrB"
                     /locus_tag="VIG70_14810"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY8251869.1"
                     /translation="MAFKLAELFPPGGDQPEAIDALVEGIRDGLSFQTLLGVTGSGKT
                     YTMAHVIARLGRPALVLAPNKTLAAQLYSEFREFFPDNAVEYFVSYYDYYQPEAYVPA
                     RDLYIEKDSSINEHIEQMRLSATKSLLERRDVVIVATVSAIYGIGDPEEYHGMILHLR
                     QGEKMSQRDVLARLVAMQYERNELDFRRGTFRVRGDVIDVFPAEHAEQAIRISLLDDL
                     VEKLEVFDPLTGRVHHPMARFTVYPSSHYVTPRETTLRAIEAIKAELRSRIDFFQKAG
                     RLVEAQRIEQRTRFDLEMLAELGFCKGIENYSRHLSGRQAGEPPPTLIDYLPQDALMI
                     IDESHVTIPQLGGMYRGDRSRKENLVEYGFRLPSALDNRPLRFDEFEKVVRQTVFVSA
                     TPADYEREKSGQVVEQVVRPTGLVDPQVIVRPASTQVDDLLSEIRDKVSRHERILVTT
                     LTKRMAEELTEYLAEHGVKVRYLHSEVETVERVEIIRDLRLGEFDVLVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSERSLIQTIGRAARHINGTAILYADRVTDSIKKAIGETD
                     RRRTKQLAYNVHHSITPKGIIKRIKDLIDGVYDASQAQVAQEEARYEAMSEKQIAREI
                     KAIEKKMLEHARNLEFEEAAAARDRLAELKKRVFGVEA"
     gene            42792..43994
                     /locus_tag="VIG70_14815"
     CDS             42792..43994
                     /locus_tag="VIG70_14815"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEY8251870.1"
                     /translation="MNAPHSPSPLASVPMAPADPILGVTEAFVADTSPKKVNLGVGVY
                     TDDHGKIPVLECVRHAESERLKSVPARGYLPIDGLAAYDLAVQELVFASQQSNIVTVQ
                     ALGGTGGLKIGADFLKHINPAAEVWISEPSWENHRQLFEAAGFKVNSYRYYDPTTRGL
                     DVTGMLNSLSALPAGSIVVLHACCHNPTGVDLDKDRWGEVLEIVRSRGLVPFLDLAYQ
                     GFAEGLDADAHAARLFAAAMSPVFLSSSFSKSFSLYGERVGALSVVTGSPEEAKRVLS
                     QVKRIVRTNYSNPPTHGCQIVATVLGNPQLRSLWDRELGEMRDRIKAMRKRLVESIHS
                     RVQNTDFSFVLKQRGMFSYSGLTKEQVRHLREEYSIYTIDTGRICVAALTSKNVDYVS
                     DAIAKVIR"
     gene            44014..44089
                     /locus_tag="VIG70_14820"
     tRNA            44014..44089
                     /locus_tag="VIG70_14820"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44047..44049,aa:Asn,seq:gtt)
     gene            complement(44121..44522)
                     /locus_tag="VIG70_14825"
     CDS             complement(44121..44522)
                     /locus_tag="VIG70_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251871.1"
                     /translation="MNRNLTTFLAVAMLGCAGHAWAQKPQPTVLTNAQLKALLSNGLS
                     VRITGADRPNTAARTGTAVYQKDGTVRFNEGPATTTHIGSWRIEGNKFCTKYSSNLGS
                     GCSNVQKIGESTYKLIPLDGLLEGTWEVVKK"
     gene            44650..46176
                     /locus_tag="VIG70_14830"
     CDS             44650..46176
                     /locus_tag="VIG70_14830"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY8251872.1"
                     /translation="MRKLDVLLLCCTLTFVAFSFPAVAQKAAAEPKPLPEDPLGRGTP
                     YGAVIGFLQAANKADYELASKYLEGKQSAKRKAELARDLHVVLNRGVKTGPDDLSKAP
                     AGTLDDGLPANLEKVGTATFESESLDVVLRLTKTPDTQPIWLFSAETLLGVTAAASQL
                     DLPWAEAIWPESFRDIRFLSYPLFTWLNVLVGVPLLVGIAWPLTRGLLRLLHPLDLRH
                     GEGVLAQIKWLLFLLILSLLARIVATQAATAEGRIFVTSVANVFIVVAAGWILVRVTK
                     LVARLKTQHLRKVGLPGKIATVELATWLFVSVWFTAASFLILRSLGFDLTAVVAGLGI
                     GGLAFAFAAQKTIENLFGTVTVVADEAIQVGDYCQVGTIEGRVENIGLRSTQIRTADR
                     VLVSVPNGQLAAGTIGNLARRDKFLFRHNILLAYETTADQLRHVLAEIRKMMLAQAKL
                     EPETVTTRLIRFGGASLELEAFAYVATRDEGVFLDTQEELLLRIMDIVEASGVRRVAA
                     "
     gene            46191..46805
                     /locus_tag="VIG70_14835"
     CDS             46191..46805
                     /locus_tag="VIG70_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251873.1"
                     /translation="MSAALQLLLVALCLVLAGTARADDWSAWQRAYDPATRTRFIPVE
                     LWTGAPWDGTQEIRMAPAALEFGQRGDKSIKGPTTWNFIQVYERVNRDKLQRFAIRDD
                     KTGLGRVFDSRYPELDCRGEVKFPLGRWKQGEVREYQLDCAGDRRPRPRPLKVTIEEI
                     DFVYGGVPHSLRFHWLFMEGRGRGTDMRYVYSPLRGLVNVQGNE"
     gene            47120..48175
                     /locus_tag="VIG70_14840"
     CDS             47120..48175
                     /locus_tag="VIG70_14840"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8251874.1"
                     /translation="MSESGGGRAGDQSRQSFLASMGQMFRRLLELHGLDAVGIARHAG
                     VDLAAIPAPGERIEVDKIDAILRVAIPLVSDPAFGVQAARCWHPSELGVLGYAWLSSS
                     TLRTGLERAARYSRLVGERGITEIEDTRQGLKVRFWANRGNPAVVPVAAVFVDIAMAI
                     LLDLCRMNAGAALRPVGASLRRRKPEPADAYARFFGCPVQFGAEENTFVLSAKDADCP
                     LPSANRQLAAVFDKMLIEELARLDKSDVVSRCRAAVLQDLSSGEGTAEDTAKQLHMSP
                     RTLQRKLAEANTTYLQLVDDTRKDLALRYIEDPRRSVTDITFSLGFSQPSAFTRAFKR
                     WTGLSPSDYRTKSPTFR"
     gene            48319..48525
                     /locus_tag="VIG70_14845"
     CDS             48319..48525
                     /locus_tag="VIG70_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251875.1"
                     /translation="MDYRLQKMDYRLFRLRDGDPIEALEAHVLARAYVDAWRALYLCE
                     PIGEHAIDSLGLAIDFGRRARRLQ"
     gene            48536..48757
                     /locus_tag="VIG70_14850"
     CDS             48536..48757
                     /locus_tag="VIG70_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251876.1"
                     /translation="MKKLHLEQAAQCGTLDQAESVLRLSAPRAWWAVERTETAVVVSH
                     IWENREPILCALIVERAPCGAQEHSLEVP"
     gene            complement(49147..49656)
                     /locus_tag="VIG70_14855"
     CDS             complement(49147..49656)
                     /locus_tag="VIG70_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY8251877.1"
                     /translation="MRLGDAVRFALLAFAAIPWLALADFSGHVVAVQDGDTLTVLAGS
                     KQTRVRLDSIDAPEMRQAFGQRSKDSLAELCMSQRAFIREHGKDRNGRTIGRVTCAGR
                     DANFEQVRRGMAWVFDRYVTDYSLYAQQDAARLSRRGLWSATNPMAPWEWRRIRRSPD
                     LAGAGSGAK"
     gene            complement(49634..50011)
                     /locus_tag="VIG70_14860"
     CDS             complement(49634..50011)
                     /locus_tag="VIG70_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251878.1"
                     /translation="MNVKRPDPDRELAEARRALAVLAERVQTTRVAADTRIHQSQVSR
                     LFRGQFKRVSSNVRKLLAYAADPKAQAAGVAEPAPTAKDAVVRAALSTWDSTPEGARA
                     LVRLLKSVRAIREVQPRAPRRRR"
     gene            50072..50953
                     /locus_tag="VIG70_14865"
     CDS             50072..50953
                     /locus_tag="VIG70_14865"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8251879.1"
                     /translation="MGGSELEKLAPPAATRHTTVLCAEVGAPPQPAARYLEVLGQAAE
                     LSGGRVLHTHPNGVLALFSTPDAAAAAAARMHVYAQALPPEQPKLDVRIGFHAGPVAQ
                     RNEDIFGDTVNLALQLADHARGGQILTSHDTASSLTPAVQGLVRPIKHLRMQGKKSDL
                     LLGELMWKDAVNQIVSARRASSPARAVLRVTYRGKAIFRRREGDTMALGRDPQCDLKV
                     DDAAVSRQHCTIARKGAVFVLRDHSSNGTFVTVAGQSEARIHDQELALGKSGLIALGQ
                     SAPTTEHVISYVCEPQN"
     gene            complement(50965..51540)
                     /locus_tag="VIG70_14870"
     CDS             complement(50965..51540)
                     /locus_tag="VIG70_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8251880.1"
                     /translation="MTRGVGIALLATALSAAAQPVAQRKEACFACHGAKGTSSTPLTP
                     SLGGQPAFFVLAQLFLFREGRRDNVLMNAAAAGLTDAELQAFADLIAKMPPPPRPAEK
                     PEIARVARGRAIAEKERCANCHNPDYSGRENMPRLANQREDYLLKALRDYKKGVRTGY
                     GMAVMPETVSGLNDQDLIDVAHFLAHFPRKP"
     gene            complement(51566..52762)
                     /locus_tag="VIG70_14875"
     CDS             complement(51566..52762)
                     /locus_tag="VIG70_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8251881.1"
                     /translation="MRVFAIAVTAILAASQALAQEKKEEPFWAIGRPKADAADKMAPV
                     PAHPVPTAADKLPLKKIKLPPGFKAEVWVSGILDARGMREGDQGTVFVSSLFVAGKIY
                     AVVDRGGKREVKTIAEKLFLPNGIEFHKGSLYVATPKDITRYDDIEAKLDNPPKAAMV
                     YDKLPGDVPHGWKFIKIGPDGKLYVPVGAPCNICEPSAAYSRIFRMNLDGTGFESVVE
                     GMRNTVGFDFHPTTKELWFGDNQRDWLSEDMPPDELNRVTQPGKQHFGYPYCHSGVMT
                     DPQFGWGKKCSDYAKPALLIGAHSAPLGMRFYTGKMFPAKYQNAIFLARHGPWNRTQK
                     YAADVVAVFIDAKGNARMEPFLTGLVEKNTYLGRPADVMVMKDGSLLVSDDHNGAIYR
                     ITYTGK"
     gene            complement(52794..53972)
                     /locus_tag="VIG70_14880"
     CDS             complement(52794..53972)
                     /locus_tag="VIG70_14880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8251882.1"
                     /translation="MLPSTTAESADEPSVILKLRRRLGGVAMAPFDVELPDGAVHRIG
                     AGAPAFSLHLRTARAARALESLDETAICEAYIDEEINFAGSFLAALDLRRIASDWHPL
                     HSLWRFMRPVLFGAVRTDKDWVARHYDYGNDFYFAFLDRRFRLYSQALYHADDEPLEK
                     AAENKLDYVMRACRLSPGSSVLDVGGGWGSFAGYAGARGAKVTALTISREQYDFLRKG
                     RVDVVYESIFAYASSRQYDAIVLLGVMEHLPNYKRLFAQFDRLLKTGGRLYMDFASNR
                     RKFNVSSFTYRYVFPGSHTPVVMPDLLAAANGTAFETIAVHNDRHSYFLTLQQWARNL
                     EAAHDELARRYGERTCRLFQLYLWGCAHQFRRDGALESYRVVFQKAYGSPSTEIGLAL
                     "
     gene            54014..>55477
                     /locus_tag="VIG70_14885"
     CDS             54014..>55477
                     /locus_tag="VIG70_14885"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8251883.1"
                     /translation="MKCFRCNSETPADAKFCPACGAGLTAAPSPQSYTPKHLAEKILR
                     SRHAVVGERKQISVLFADMKGSMELLAERDPEEAHNILDPMLRHMMDAVHRYEGTVNQ
                     VLGDGIMALFGAPLAHEDHAVRACHAALRMQQTVGWYAEELRRGRGVDVQIRVGLNSG
                     EVVVRAIDSDLHMDYSAIGQTTHLAARMEQLARPGSTLISRETLRLAEGYVEIKALGP
                     VPVKGMPEPVEIFELVRSEQVHSRLEASAARGLTPFVGREAEVDALHQAMARAAAGHG
                     QVVAVSGEPGVGKSRLFWEFTHSHRAHGWLMMEAQATSYGKNTSYLTVKELLQNYFHL
                     EERDDVRRVREKVTGKLLTLDESLRPTLPAFLQLLDVPVDDAEWQTPDLPQRRQRMLD
                     AVKRLILREGQVQPVLLVFENLHWLDSESQAFLDSLVDSLPLARALVLVSYRPEYQHG
                     WARKSYYTQFRIDPLPPQSSEELLRALMGDDPGLGPLK"
BASE COUNT         9011 a        18875 c        18057 g         9534 t
ORIGIN      
        1 catccagccg gatgaggtcg acgcccgaag cgaaatccgc gatcacttcg gcgcccgcgt
       61 cgtcaaacgc gttcttgatg atgaagctgt cggcccccgc gccgccgatg agggagtcgg
      121 acccgtcatc ggtgaactcg ccgccggcga gcgtatcgtc gcccgcgccg ccgtcgatgg
      181 tgtccgcgaa gcccccgccc acaatgctgt cgttcccgcc ccggccgtcg agccggcttg
      241 ccgcataggt cacgtggatg cggttgtcca gctcgttgcc gatggccgtg acgccggatt
      301 cggacacgtc gttgttgatg gtgagattct cgaagccggc ggcgagggtc cagctcctgt
      361 cgtagacgat cacgtggtcg atgccgcccg cgtcaatcag tacgtcgaag ccgttgtcga
      421 catggaaaac gtcgtcgccc agaccgccgt ccagcgtgtc gggcgccgag ctgacgaaat
      481 ccggtcccgc ggcgctcatg agcgtgtcgt tgccatcgcc gcccacgaga tggtcgctgg
      541 cctcggtaaa atcctcttcg ttccacccgt cggacagcag atcgtttccc gcgccgccga
      601 tgagcgaatc gctgccgccc atgccgttga tcgtgtcgtt gccgccgagc ccgtcgatgg
      661 tgtcattgcc gctcgtgccg gtgagcgtat cgtcgcccgg cgtgccgttg atgacttgcc
      721 ctccgccgga tccgccttcg accatgatgt cggtcgcgac gagcgtcgcg ccgctttgca
      781 acgtggcaat caatagcttg gcaccggcgc cgttgccgtc ggcgtcgtag aagacctgct
      841 gcgtggcggt gttgtagagc acgcggtcgt ctgcgtcggt ggcggtgccc gtggagttcg
      901 ccttgaagcg tgcgtcgccg gttgcgaagt tgccggacac gccgagcgtt gccatgaccg
      961 tgccgtcgag cagcaacttg tccgatcccg aggcccagtc gccgatcgtg tcggcgttcg
     1021 ccgtgccggt ctcgcggaag atgaacgtgt cgccgcccgt gccgccccac agcgtgtcga
     1081 cgccactccc acccgccagc gtgtcgaagc cggcgcgcgc ggagaagctg tcggcaccgg
     1141 cggtgccggt gaggctgtcg ccgccgtcgc tgccccatac acgttcgatg ttcacgaacg
     1201 tgccgctaaa gccgccgctc agcgttcccg cggacaggtt caccgtgagg ccggtgaccg
     1261 tggccctcag gtccagcgtg tcgaagcccg tgccgccgtc atagaaatcg gtgccggcgc
     1321 tgcccgcaag ggtgtcattg ccgcccaagc cattgatggt gtcgttgccg cctgtaccgg
     1381 cgaggttatc gttgccggcc gtaccgttga tgagcgccac acttcctcct gcgagggatc
     1441 gagtcgcagg agtctgtgat gccggctcgg accggtcgat agtcctgtta gctcagtcga
     1501 tccggagctc gactttgccc agtccctcaa cgtcgaacac cacgtgatcg ccggttgtca
     1561 cggtcttgct ggtgacgatg ctcccggtaa tgacgacatc gccccgtagc agcccccgcc
     1621 cgagcgaagc gaggtggtcg gcgagccacg cgagcgcatc caggggatgg cccatgacat
     1681 cggccccgcg cccctctccg accttgtttc cattgattgt tgcgatgccg cgcaccgcgg
     1741 caaggtcgat ggccttccag tcctcgaccg gcgcgccgaa cacagcgcct tcgttccagc
     1801 agttgtcggc gatgagctcg aacgggtgct tcgggagcgc cttgtagtcg gcattgcgat
     1861 cgtccgccag ctcgagcgcc ggcatgaccg cgcccaccga ctgggctacg cgctcgcgga
     1921 agaatggctt atcggccgcc ggcaggtcgt ccgcgacctg gatcgcgatc tcgaactcga
     1981 cgatcagccg cacgtagtcg gcggcccgga cacgcgccgg cgagcgatgc agcgtagaca
     2041 cgaacatcac gccggcctgc ggcgactcca cgccgacgaa gcggcgcatc tcggccgagg
     2101 tcagcgcgat cttgtatccg gcgagcgcac ccagcttcgc agcgcgcaac gcgacaaacg
     2161 cgtcctggat ggcgtaggcc tcgctttcgg tgcgcggcgc gagatccggc ggcagcggcg
     2221 tgaaccgctc gcgccgctcg tgcgcttcaa ggagtcttct tgctgcctgg gtggacatgg
     2281 ccgcacgccg ggcatttccg ggtgctccga tcgaaggcgt cgaagagcgg aggcaggtcc
     2341 gtgacgatgt tttgcacgtt cacgtccacg cggtgcacca gcgcccagca cttcgggcag
     2401 taccactcga agccgtcgtc gacgtcgcgc ggccggacct tctccaccac caggccgacc
     2461 gtgccggcag gacgctgcgg cgagtgccgg cgacggccgg gaacgagcag gatctcgcct
     2521 tcgcggatgg ggatgtcacg cggcttcccc tcttcaatca ccttgagaat catgtttccg
     2581 cgcagctggt agaagaactc ctcgtacggg tcgtcgtggt agtcgctgcg cgaattcggc
     2641 ccgccgatca cctggacgat caggtcctcg gcctcgcgga acaccatctg gttgccgacc
     2701 ggcggcttga ggaggtgggc atgctcgtcg atccagcggc tgaaattgaa ggcggcgagc
     2761 atggcccatt ctagaatacc gtccgatgaa gctgggtcaa cgcaaatacg ccgatattct
     2821 ggtcctctcc ccggcggggc ggctcgatca cgacagctgc gacactttca gcgctgagct
     2881 gaagcctttc ctggacgacg ccgtcgccgg gcgccggatc gtgctggacc tctccgggct
     2941 cgagtacgtg tcctccgccg gcctgcggct cctgctcctc gccggcaagc aggcgaactc
     3001 gcaggacgcc aagctcgtga tcgccgcgat gcggccgctg gtcgccgaga tcttccacat
     3061 cagccgtttc accatgctgt tcgagatcca tgccggcgtg cgcgaagcgc tgatggccgc
     3121 ctcgccccag gccgccgctg ctttcgacct cgggtagcgc cgggatgcgc gtgcgcttct
     3181 gggggacgcg cggctcgctg ccggtcgcgc tgacctggcg cgacgtgcgc gaccgcctgg
     3241 cgcgcgcgct ggtcgtggcg aacggccgcg gcctcgatac cctggaaaag gcgcaggcct
     3301 tcgccgagca cgagctcggc tttccgctga cccataccta cggcgggcac acctcgtgcg
     3361 tcgaggtcga gctgccgcag gcgcagcccg acgagtattt cgtctgcgac atggggagcg
     3421 gtgcgcgccc cttcggtgag cacgtggcgg cgcgccaggc cgggcgtgca gccacggtca
     3481 acgtgttcat gtcgcacatg cactgggacc acatcatggg gttccccttc ttcgggccgg
     3541 cgtaccgggg cggcaaccgc atccgcatcc acggctgcca cgcggcgctg gagcaggctt
     3601 tccgccggca gcaggatccg ccttccttcc ccgtgccgtt ctcgcagctc gccgcgcgga
     3661 tcgagttcgt gcgcctcact cccggcgagg cgttcgagct tcccggcggc accgtgacgc
     3721 cgcagctcca ggaccattcc ggcgactcgt acggctttcg gttcgagagc cgcggcaaat
     3781 cgctcgtcta caccaccgac tcggagcaca agctcgacga ccaggaggcg cggcagcgca
     3841 tcgtcgagtt cttccgcgag gccgatctcg tggtgttcga ctccatgtat tcgctcgccg
     3901 aggcggtttc ggtcaaggcc gactgggggc attcgagcaa cgtcgtcggc gtcgagctgt
     3961 gccaggcggc gcgtgcgcgc aagctcgccc ttttccatca cgagccggcc aatggcgacg
     4021 acgccatcga gcgcatcctg cgcgagacgc gccgcctcga ggagttgacg cgcgacggcc
     4081 acccgctgga agtcatcgcc gcctacgacg gcctggagat cgatctctga gcgtcggcgc
     4141 gggcccgcgt gcgctcggct cgacggtagc agcggtctgc gtgctgctcc tcgcgctcga
     4201 cctgccgggc gtggcagtgt ggcgagaagc cctgttcgat gcctaccaga ggatcctgcc
     4261 gcgcagccgc gtctcggcgc cggcggtgat cgtggcgatc gacgaggatg cgctcgaggc
     4321 ccgaggccag tggccctggc cgcgcagcct ggccgcggac ctgattcgcg cggtggccga
     4381 cgccggcccg gcggccatcg gagtcgacct gttgttcgtc gagcccgacc gctccgctcc
     4441 cggcgccgac gcgctgttcg cgcagacgct ccaaggccgc aaggtcgtgc tcggcatcgc
     4501 ggggctggac taccgcgacc gacgctcacc gctgccgcca aagtcggcgc cggtgcgccg
     4561 gtcgagcggc cgcgagctgc cgctgcggag cttcgaaggt catctgcaaa gccgcgccga
     4621 gatcgatcgc gccgcggcgg gccggggact tctcagcgtg gactcgcccg accgcgtgat
     4681 ccggcgcgtg ccgctgatcg cccgagtcgg cgaggtcgtg gttccggcac tgtcggtcga
     4741 gatggtgcgc gtcgccgcgg gcctgccggc gctcggcatc gaggaccgcg gcggccagcg
     4801 cgtcgcgctg gtcctcggcg acctggcgat cccggtgcag tcggacggca cgctgcggcc
     4861 ctacttcggg cggcacgatc ccgcgcgctt cgtgtccgcc gaggaggtgc tgagcggcat
     4921 ggcgccgcgg gagctgctgc gcgacaagct ggtcctcatc ggcgtcaccg gcctcgggct
     4981 cctcgacttc caggcgactc cgctcggcga gcgcatcccg ggggtcgaag tccacgccca
     5041 gatcctcgag cagatcttcg acggcgtgta cctgctgcgg ccgactgcag ccgccctgat
     5101 cgaggccgcg ctgcttgcgg ccgcggcgct tgctttcgtg gtgctggtgc cgcgcgcgcg
     5161 tgcggcaacg tccgcactgc tgctcgccgg tacggttctt ctttttgtct ttatcggctt
     5221 gtttgcgttc aaggcggggt tcgtgctgga cgttgccggc ccggccatag gtgcgctcgc
     5281 ggtgttcggc gcattgctcg ccgccacctt cgccgaagcc gaccgccagc gccgggcgct
     5341 gcgcgaagcg caggcgcggg tcgccggcga gatggaagcg gcgcgccgca tccagatggg
     5401 cttgctgcct gccccgcgcc gcatctttgc cggcgacggg cgcttcgcgc tcgacgcggg
     5461 tctcgagccg gcccgcgtcg tgggcggcga tttctacgac tgcttcatgc tcgacgatcg
     5521 gcgcctgttc ttcgtggtcg ccgacgtctc gggcaagggg ctggcggcga gccttttcat
     5581 ggcgcttgcc aagtcgctcc tgaagagcat cgcgctgcgc ggcggcgccg atccggctgc
     5641 gatcctcacc caggcgagcg ccgaaatcgc gcgcgacaac acggaatcgt ttttcgtgac
     5701 cgctttcgcc ggcgtgctcg acgtgcgctc gggcgtgctc gaattctgca atgccggcca
     5761 tgagccgccg tacctctgcc ggcccggcgc cgcccccgag cgcctcgagc acccgggcgg
     5821 cccgccgctg accgtggtgg agggcttcgc ctacccctgc ggccgccgca gcctgtcgcc
     5881 cggcgagacg ctgtgcgtgg tgaccgatgg cgtgaccgag gcgatggacc gcgccggcgc
     5941 gttctacggt gccgcgcgcc tgcggacggt gctggggaag caacccgacg taaccgcagt
     6001 gcgcgaagat gtacagcgct tcgccgccgg cgccgagcag gcggacgacc tcacgctgct
     6061 ctgcgtgcgc tggaacgggc ctgccagcta gcggacgttg atctcgacgc ggcggttgcg
     6121 cggctcgtcg acgccgtcgg cggtctgcac caccggctcg cgctcgccgc ggccggccgc
     6181 gcggatgcgc tcggcggcga ttccctgcgc gacgaagttt tcgcgcacgc gctcggcgcg
     6241 ctgcagcgac aggcggtcgt tgtcgtcttc attgccgacg cggtcggtat gcccgatcac
     6301 caggatgtcg ggcgaagggc ggcggcgcag ctcttccagc atgcgcgcga gctccccttt
     6361 cgattcttcg gtgagctcgt cgctgcccat gacgaagtag agcaggaacg cggccgggcg
     6421 ggctggaatc gccgcagccg tggcggcgaa agcgctgcgc acttgcccgt cggtgagctg
     6481 agcggggccg ctgtcgcggc cgatgcgatg ggctgcgtag ggcttgtcga gcaccgtgcg
     6541 cttgccgccg tgctcgacga ctacggcgcc ggtgtgaccg tctgcgctcg ggaccaacac
     6601 cacgagctcc gccgctggcg acggaaccga aggctggctg aacacgatga ccagcgccat
     6661 gatggcgagc accgccacca tggtttaccg gcccggctcg gtaaccttga cgaagaactc
     6721 cgtgccgcgc acgcccatga tgctcgaggg cgtatgcacg cgcatcgact cgggggagcg
     6781 cttgaccatc cggcccgaga ccacggcgag cgtgccgcgc ttgagcgagg cgtcgaactg
     6841 gccgtcgtgg gtggtgctgt cgaagctgta gcggtcgatc gcgagcacgc tgccgggccc
     6901 gatcgaaagc aggctgttgt cgaggaaggt gaccccgacc gagccgtcgg cgccggtgac
     6961 cagcacatcg gactgccgca cgctcatgcc ctgcgccgcg gcgagcttct gcccgtcgcg
     7021 ctccacgtgc accgtaccgc gcatgttctt gatctggccg acgtcgttcg cgtaggaaac
     7081 tgccgcgaac gccaacgccg ccagggtgac aatccaacgc atcgtgacct ccctaccagg
     7141 tccgtagacg gccaattcta taatgcaccc ccacctcccc gtgtgcacta tgtccgcgct
     7201 ctcacgatct accatgttcc aggcccggcg cgaggaattg cacggcttgc gcgccttcgt
     7261 cgaggcattc tgcgcgcagg cggccatggg ccgggacgcc tgcctgcgcc tcaacctggt
     7321 gctcgaggag cttttcacga acacgcttcg ccacgggcac cgcggcgact ccgacgcgcc
     7381 cgtctgggtg agcttgtccg gcgggcagga ccggcgcgtg acggtgagct acgaagacac
     7441 cgcgcggccg ttcaacccgt ttccgcgaaa gtcgccggat acgacgcagg agctccacaa
     7501 gctcgaaggc cgcggcgtgc tgctgctgcg cacgctctcg gtgaagcgcg agtacgccta
     7561 cctcttcggc cgcaaccgca tccgcgtcgt gctcgatacc ggttgagagg ctggtgccgg
     7621 ggccgctgac gggcggtgcc cgtcacgata aggaatcggc acgtcgggaa tcctgaagcc
     7681 ctgccctagg tgcggtaaga agcggggcga agtcgtcgag cggggcgcgc ctagttgcca
     7741 cgttgcttct tgtcagcgtc attcggagga ggtctgtctc gagtatccag acctacggca
     7801 cgccacaaca gctccgccca aacccacgcg gtcgctagcg aaacagtaat gatgaaaagc
     7861 caccaacggg gcccgcccag ctccccgaac atccacaagg cgctaatgca caaaatgctg
     7921 ccaacgctcg taatgacgaa cgaaacaaag ttgctcttat ggctcaggta ctcggcgaat
     7981 ttgacacgcc aatgcatgcg cgaactaggt ggtatgtggc gtcctggtga tttctcttct
     8041 tcttgaaggc catgatgcgg acgcagcaac cgctggctag ttacatgcgt caccgaatgg
     8101 acagcgtcgg tgtgcgctag cgcacagctt tgagccgata gtcgcttgca agcgcgacag
     8161 cagtgagcag cgaaggggtg ttggtgcccg gggccggact ccaaccggca cggccttgcg
     8221 gccaagggat tttaagtccc ttgcgtctag cagtttcgcc acccgggcgg cggatagaat
     8281 tttgccatgc ccgacgtccg gcgcctgatc gacgaaggct tgctcgacga agcggcgcgt
     8341 cagctcgaag acgctcccga cacccgcctg atgctacgcc tggcggcggc gctccaggac
     8401 cgcggccgcc ccgacgacgc gctcggctgg tatcgccgca tcctggaggt cgatccgggc
     8461 aacggcgacg cgcttctctg cctcgccgtg ctgcacgagg aagtcgacat cgagcaggcg
     8521 cgcggcttca tggatcgcta cgtcgcgctg cgcggcgacg ccgcgggccg actgcgccgc
     8581 gcgctgatgc tgccggcgat cgcccagtcc ggcgagcaca tcgagcgggt cacgaagcgc
     8641 ctggaagccg agctggacga gctctcgaac gggcgcttcg cgccgatccg ccagcccgag
     8701 ttcgaggtcg gcgcgacgcc cttcttcctc gcgtactacg ggcgcaaccc gaagccgctc
     8761 ctcgagaagg tggcgcgcgc ctgccgggcg gtctatccca ccgagacgcg ctgcgagcgc
     8821 aagctcttcg cttccggaaa aaagctgcgc atcggcttca tctccgcgca cttcagcgag
     8881 cactcggtgg cgaagacgat gtggggtttc gtgcgcgacc tgccgcgcga aagcttcgac
     8941 gtgcacgtgt tcgcgatcgc gcccgccggc gacgcatgga gcgagcgcct gcggcgcgac
     9001 gcggaccact acgccgccct gccggtggac ctcgagcgcg cgcgcgatgc gatcgcgtcc
     9061 gcaaagctgg atatcgcgtt cttcaccgac atcggcatgg acccgctcac ctatttcctg
     9121 gcgttctggc gcctcgcgcc gatccagctc aatagctggg ggcattcggt gacgagcgga
     9181 atcgacacca tcgactacta cgtgtcgcac gacggggtcg agctgccgga ggcgcagtcg
     9241 cactacagcg agaagctgat ccgcctgccg ggctactgca tgccgcgcta tctgcggcct
     9301 gcgctcgccg gggcgaagaa gacgcgcgag gcgctcggcc tgccgccggc caggcgcctg
     9361 tactactgcc cgcagaacct gttcaagttc cacccggatt tcgacgccgc gctcaaggcg
     9421 atcctcgagc gcgactccga aggcgaagtg gtgctggtgg actcggcacg gccctggacc
     9481 ggccagctcg cgcagcggct gagccgcacg ctcggcgcgg tgggcgcccg ggtgcgcctg
     9541 ctgccgcgaa tgcggcatgc cgacttcctg cagcacctct ccgcggccga cgtggtgctc
     9601 gatccgttct acttcggcgg ctgcaattcg agctgcgaca ccttcgcgct cggcgcgccg
     9661 atcgtcacgc tgcccggatt cctcctgccg ggacgattca cgttggggtt atacaaggaa
     9721 atgaatattg aggagtgcgt ggcgcgttcg cccgacgaat tcgtcgacat cgcgctgcgg
     9781 ctgggccacg accctgccta tcgcgaagag atttcccgca gaatctccag gaacgccgag
     9841 cgcttgttcg accgccccga ttgcggaagg gcgctcgggg cggcgctgtt acggattgcc
     9901 gaggaaagaa ggtaaggcgc gagtcggaat cgaaccggcg tacacggctt tgcaggccgc
     9961 tgcatgacca ctctgccatc gcgccataag caaaaaggga aggccagcgg gccttccctc
    10021 gcagattctg gagcgggaaa cgagtctcga actcgcgacc tcaaccttgg caaggttgcg
    10081 ctctaccaac tgagctattc ccgcgtcacg tcgggccaat tataagtcaa ccgcgcaccg
    10141 aagcgtaagg cagcgccttg cgcaggtagt agagcatcga gatcaccgtc agcaccgccg
    10201 cgatgttgat gaggacggtg cccagccact ggcagtccag ccccagcagc acgtcgtgga
    10261 acagcagcag cgggatcgcc accatctgcg ccaccgtctt cagcttgccg atgaaggcca
    10321 ccgctacgct cttcgcctgg ccgactttcg ccatccattc ccgcaacgcg gaaatcgcga
    10381 tctcgcggcc gacgatgatc agcgccacga tcatgtccac gcggttcagg tagagaagcg
    10441 cgagcagcgc agccacgacg atcagcttgt ccgcgaccgg gtcgaggaac gcgccgaagg
    10501 ccgacatctg gttcagccgc cgcgcgagat agccgtcgag ccagtcggtg atgccggcga
    10561 agatgaagat cgcggtcgcc gtgaggttca tcgtttccgc agagaaccag ggcaggtagt
    10621 agatgccgat cagcagaggg atcaggatga tccgcgacag cgtgaccagg ttaggcgcgt
    10681 tgagcgggat catgcggcag cagactcgtg aaggtgccgg tagatgcgtt ccgcaagcgg
    10741 ccggctgatg ccgtcgacct tggcgatgtc gtccaccgcc gcggcctgca cgccgcgcag
    10801 gccgccgaaa tgctcgatca gcgcctggcg ccgcttggcg ccgattcccg ggatctcgtt
    10861 cagcatcgag gtcgtgcgtg ccttgccgcg ccgcgcgcgg tgaccgacca cggcgaagcg
    10921 gtgcgcttcg tcgcggatcg cctggatgag gtgcagcccg ggatgcgcag gcgcgagctg
    10981 cagcgtgcgc gcttccgact ccaggatcaa ctcctcttcg cccggcttgc gcccgggccc
    11041 cttggcgacg ccgaccacgc acgcctgatg caaccccaga tcatgcagga ccgcggacgc
    11101 gatcttcgcc tgccccttgc cgccgtcgat caggatcagg tcgggaatct tgcccgcctc
    11161 ggccgtgacg cgctcgtagc ggcgctcgag cgcctgccgc atggcggcgt agtcgtcgcc
    11221 gggggtaacg tcgcggatgt tgaagcgccg gtactcggct ttctgcatct ggctgcgatc
    11281 gtaaaccacg cacgaggcca cggtcgcctc gcccatggtg tggctgatgt cgaagcactc
    11341 gacgcgctgc gccccctccg gcaggccgag cgcctcgcgc agcgcgagca ggcggccttc
    11401 ctgcgtcgcg cggtcgcgca cacgctgcgc aatcgcgagc agcgcgttct tgcgcgccat
    11461 gtcgagccac accttgcgct ccccatgcga ggggttgatg acgtggattc cttcgatctc
    11521 gaccgcttcg ctcgtcacga ccagccccgg cgcgggctgc tccgcgtagt gctgctcgag
    11581 gaacgcggcg atcacctcgg ccgccgccgc gccttcggcg ttcgccggaa agaagctgcg
    11641 gtcgccgacg tgccggccgg tgcggatcat caccaggttg acgcacgcga tctcgccgtc
    11701 gatcacgcag gcgacgacgt cggcgtcgac gccgcggttc gactcgacgt attggcgcgc
    11761 ctgcactcgg ctcagcgccc gcacctggtc gcggtaggcg gcggcctcct cgtagcgccg
    11821 cgcctccgac gcggcgttca tcttgcggtt caggttcccg accacgtcgt cttcgcgtcc
    11881 ctcgaggaac agcgccgcgt tcgccacgtc ttcggcatac agcgccgccg tgatcctggc
    11941 cgtgcagggc gcggtgcaac gcttgatctg gtgcagcagg cagggccgcg agcggtgctc
    12001 gaacaccgtg tcctcgcagg tccgcaggcg gaacacgcgc tgcaggagct ggatgctctc
    12061 gcgcaccgcg taggcgtgcg ggaacggccc gaagtaacgg tggcgtttgt ccttcgcgcc
    12121 gcggtggaac ccgaggcgcg gaaactcgtg gccggtgatc gtcaggtaag gatacgactt
    12181 gtcgtcgcgg aacaggatgt tgtagcgcgg cgcgaggctc ttgatgaggt tgttctcgag
    12241 gagcagcgcc tcgccctcgg agcgcgtcgc ggtgacctcg atcgacgcca cctgcccgat
    12301 catcatctgc gtgcggggtc cgtgcgcctg cttgttgaag tacgacgcga cgcgcttcct
    12361 gaggtcgcgc gccttgccga cgtaaagcgc ctccccgccc ctgcccagca tgcggtagac
    12421 gcccggcaga ttcggcagcc cggcgacaaa cgatttggcg tcgaacacga tgatttcatt
    12481 atagatcgtg taaaattcgc gccttttcag cggcggtgtc atggcgaagg tcgaagcagt
    12541 cggtatccgt cccggcaacc tcctcgagtg ggataagcgg gtctggcgcg tgctcaagtc
    12601 ctaccacgtg cacgtcggcg gccgcggcgg cgcgttcatg caggtcgaga tgaaggacat
    12661 cgagaccggc accaagacca atcagcggtt tcgcaccgag gacaaggtcg agcgggcgtt
    12721 cgtcgacccg cgcgacatgc agtacctgta ccaggacggc gacagctacg tcttcatgga
    12781 caaggaaaac tacgagcagc tcacgctgcc cgcggagttc ctcgagggcc aggcgggcta
    12841 cctgctgccg aacaccgacg tccagatcaa cttctacaac agccgcccca tcggcatcga
    12901 cctgccgccc tccgtggtcc tcacggtggc cgacaccgag cccggcatca agaactctac
    12961 cgccaccaac tccttcaagc cggccacgat ggagaccggc atcaccgtcc aggtgccgcc
    13021 tttcatcaac aagggcgaga agatcaagat cgacaccgcc gaaggcacct acatggagcg
    13081 cgcctagcga ttcaggctag cgcggtcagg gcttcctggg cttcacggta ggcgacgctt
    13141 ttccgcagat ctcgcccgcc cttcttccgc atccgctcga gccgttcagg attcttcatc
    13201 tggagctcgc ccagcgactc cagcagcttg ttctgtcctt ccgggtcgtt gcatagcagc
    13261 accatgtcgc agcccgcggc gagcgccgcg cgggcgcgct cgggcgggcc gccgacggtg
    13321 ctggcaccag ccatcgaaag gtcgtcgctg aagatgaggc cgtcgaatcc gagcttggcg
    13381 cgcagcactt cctgcagcca gtatttggaa tagcccgcgg gctcggggtc gcactgcgtg
    13441 tagatcacgt gcgccggcat gactccggcg agccctgcct ggaaggcggc ccggtacggc
    13501 gccacgtcct tcttcaggat ctcggaaaaa gctctttcgt ccttcggaac gtccacatgc
    13561 gagtcggcgg cggcaaagcc gtggccgggg aaatgcttgc cgaccgccgc catgccggcg
    13621 ctggcgaagc cctgcacgat ggcggcggcg agcgcaccca cggcgttggg gtcgtaatgc
    13681 agcgctcggg tgccgatcac cgagctcgag ccgtagtcga ggtcgaggac cggcgcgaag
    13741 ctgaagtcga tgccgtgcgc gccgacctcg gcggcgatca cgtacgcagc gccgcgcgcc
    13801 gcctccttcg ccgcttcctt gtcgcggtcc cacaagcgac ccagctcgcg catcgccggg
    13861 atcgcggtga agccttcgcg gaagcgctgc accctgccgc cttcatggtc gacgcagacc
    13921 aggagagcgg gttcacgcag tttctcaata tcctcaacta acatgctgag ttgttgagtg
    13981 ttttcgtagt ttcgagagaa caggattacg gcgccggccg cggggtgccg cagtcgctcg
    14041 cggtcctcgt cgctgacctg cgttcccacc acgtcgatca ccgccggccc gagcgggcgc
    14101 cttacttttc gcactcgagg atcaccgtgg cgaccgcgac gccgcgctcg tcggcgaggg
    14161 agacgtgggc gcgggaaatg cctcgccccg ataacaattt ttggagctgc ttcgaaaatt
    14221 ccagctctgg cttccccgaa ggccgattca tgacccatac cccgtgccag ttcgccggcg
    14281 ccttgatgcc ggtccccagc gccttggtga aggcttcctt cgccgcgaag cgcttggcga
    14341 ggtacgaagc cggctgacgg tgcccgagga accgcttcag ctccggctcg cacaggatgc
    14401 gccgcgcaaa gcgctcaccc cagcggccga gcgctttctc gatcctgccg atctcgacca
    14461 cgtcggtgcc gacgccgaag atcacccggc ggcctgccgc atcaggctct tcatctcccg
    14521 cactgcctcg cgtatgccga caaaaaccgc gcgcgacacg atcgcatgcc cgatgtgcag
    14581 ctcgcgcacg ccgggcagcc gcgccaccgg ctggacgttg aagtagttca gggcgtggcc
    14641 ggcgttgaag cgcatgccga gcgagcggat cgcatcccct gccgcctgga tcttcttcag
    14701 ctccgagacc acggccgggc tctccgcgtc gcgccccttg gaatggaagg cgtgggcata
    14761 tgggccggtg tgcacctcgc agaccgaagc gccgatccgc ttcgccgcct cgacctgggg
    14821 gagctcggcg tcgatgaaca ccgagaccac gatccccgcg gcgctcaatt gctgtactgc
    14881 ccttttcaat gacttctcct gggaaaccac gtcgagcccg ccttcggtgg tcacctcgtg
    14941 gcgaccctcg ggcaccagca tcgccatttc cggcttgatc cggccggcga tcccgaccat
    15001 ctcgtcggtc gcggccatct ccaggttgag cttgatgtgg gtcagctcgc gcagccgccg
    15061 cacgtcctcg tcctggatgt ggcgccggtc ctcgcgcaag tgcaccgtga tgccgtcggc
    15121 gccgccgagg tgcgcctcga ccgccgccca caccgggtcg ggctcgtagg tgcgccgcgc
    15181 ctgccgcacc gtcgcgacgt ggtcgacgtt cacaccaagt tcgatcatct tcgatttgct
    15241 tccgtcataa atcggtgagc tcccgcagca ccgcgcgcgt gtgcagcacc tggccatgca
    15301 ggcgctggcc gatcagcgcg cgcagaagca tgcgcgcctc ctcgcgggtt tccggccggg
    15361 cgtactcgtc ggccgcgagg tcgagcagcg tctggccgct caccacgaga tccccgttgc
    15421 cggagccgcg gctcggtacg ggtcccttgt ccggttcata gacatagcgg cggccgggct
    15481 cgatcggcgc gccgctcgcc gcctcgcgct cgaggagcgg agcatacccg agctcggcca
    15541 gcatccgttt ctcgaagctg cggagcacgg aagcgtgcgt ggcgccggcc gacaggcccg
    15601 cgagggcttc gccatagaag tcgaagagcg cctcgtgcgc atcgtcgcgc ggcagcaggc
    15661 gcagcaggag ctcgttcagg tagaagccgc acatcagccc gcgccccgcg aggggttgca
    15721 gagcgccaga ccactccgcc gagatcaggt ttcccaattc tgcagaggcg gaccagccga
    15781 gccgcagcgg gtggaacgac agcagcacgc cgcgcatcgc cgagcgcggc cggcgcgcgc
    15841 cgcgcgccag cagcgcgacg cggccgaagc cgcgcgtgaa cgcctccacg atcaggctgg
    15901 tctccttgta cggatacgtg tgcagcacgt agcccggctg gtggtcggcc cgtcttttca
    15961 ttcgtaccct agctgtcgca gcacgcgcgc atcgtcggtc caagccttgc gcactttcac
    16021 ccacgtgccc agatacacct tgcccccgaa aagcttctcc atgtccttgc gcgccgcgct
    16081 cgccatgcgc ttcaggcgct cgccgcccgc gccaagcacg atcggcttct ggctgtcgcg
    16141 ctcgaccagg atggtggcct cgattcgccg cagccggcct tcttccttga aactgtcgat
    16201 caccacctcg cagcggtacg gcatgtcttc gcccatcgcc tcgaacagct tctcgcgcag
    16261 gagctcggcg gcgaaaaagc gctcgtcgcg gtcggtgagc tggtcgggcg ggtacgccgc
    16321 gggcgcctcc ggcaaggccg aacgcagcgc ggccagcagc tcggggaggt tcttcccggt
    16381 cttcgcgctg agcgggacga tcgccgcgaa atcccgggtt ttcgacaagc ggtcgaggaa
    16441 cgggatgagc tgctcgggat tctttagccg gtcgatcttg ttcaccgcgg cgacgacgcg
    16501 ctgctccgcg ggaatcttgt cgagcgtcat ctgatcgtcc ggcccgaagc gcagcgcctc
    16561 caccacgaag agcgcgacgt cggcgtcgcg cgccgcctgg ctggcgcggc ggttgagccg
    16621 gcgatgcaga gtgttttttg cctgcgcctg ctgccccggc gcgtcgacga agacgtactg
    16681 gcagtcgggg cgcgtcagga tgccggtgac gaggtggcgc gtggtctgcg gcttggacga
    16741 gacgatcgac agcttctcgc ccacgatgcg gttgagcagg ctcgacttgc cggtgttcgg
    16801 ccgcccggcg atgccgatcg tgccgcagcg atacgcgctc atagctccag cttgttgagt
    16861 atggctcttg cagcctcttg ttccgcgcgc tgacgcgact tgccctcacc cctcgcttgc
    16921 agtccctcga cgacgcattc cacctcgaac gagcgctggt gcggcgcgcc gtgggttgcc
    16981 acgacgcgat attccggcaa cggcttgccg cgagcgtgca tcagctcctg caggcgcgtt
    17041 ttcgcgtcct tgtccacctt gccgggatcg agggtctcca gcagcgggcc gaaagcctgc
    17101 accaccgcgc gccgcgccgc cgggtagccg ccgtcgagga agatcgcgcc gaaaatcgcc
    17161 tccagcgagt ccgccagcat ggaatcctgc acgctgccgg cgcgcaggcg cgccgcgacc
    17221 cccaatgcct tggcaacttc aaccagcgac tcctcgcgca ccaggctcgc gcgcatctgg
    17281 gtcagcttgc cttcggggag ctgcgggaag cgggcgtaga gctcgtcggc gacggcgcag
    17341 ccgagcacgc cgtcgccgag gaactcgagc cgttcgttgt ggtccggacc gtgactgcgg
    17401 tgcgtgagcg cccgctcgag gagctgcgcg tcggtgaacc ggtatccgag cagttctact
    17461 tagccacctt tcgctcagtt accgctcgct cagttacctc gcgagttggc cgcgtagtcg
    17521 atgtacaggc caaccgcccc gaacagcggc acttccttgc ggtaggcgaa cgcgatcacc
    17581 atctggttgc catcccgcgt gatctcgagg tcctggggcc tgatcgtctg gatgttgtcg
    17641 atcgccgcgc ggttctcgaa ggcgcgccgc acttccgccg gattctggga cgagcgcgcg
    17701 atcagctcga tcgcggtctt ggcggtgcgg aactcgagga acgacggaat gaccttcatc
    17761 gccaacagcg ccagcatgcc ggcaatgatc aataccacca gcagccccgt gatggttacg
    17821 ccgtgttgtc ctttttgcat tgttgtgcct ccctcagctt tcagcggaac gagccgaagc
    17881 gtcccagctc gttcaggttg agccagatga agaacgcctt gccgacgatg ttggcgtcgg
    17941 gcacgaaacc ccagacgcgg ctgtcgctcg agttgtcccg gttgtcgccc atcacgaagt
    18001 agtgccccgg cggcaccgtg cacgccaggc cgttcgtatt gtagttgcaa tttccggcga
    18061 acgggaacgc gcggcccggc atcatcgacg gcggcgagtc ctcgtcgagc aatatttcat
    18121 gttcgacgcc gttcaccgtc tcgacgtagc ggcgcgagaa ctgcatgcgc tccttcgaca
    18181 agtagtcgtc gacctgccgg gtgggcaccg gcgtgccgtt gatcgagagc cgcttgttgc
    18241 ggtactcgag gcggtctccg gggatcgcga ccacgcgctt gatgtagtcg agcgacggat
    18301 cttccgggaa gcggaacacc atcacgtcgc cgcgctccgg gctgccgagc tcgaccactt
    18361 tcacgttggc caccggcagc cggatgccgt aggtgtactt gttcacgagg atgaagtcgc
    18421 caaccagcag cgtcggcacc atcgagctcg acgggatctt gaacggctcg atcaggaacg
    18481 agcgcagcag gaacacgatc aggatcaccg ggaagaagct caccgagtac tcgacccacc
    18541 agggctgctt cgcgtgctgg gcgcgggagc ggcgcagcca gagcatctcc atggcccaga
    18601 cgccgccggt gaccaccagc aggatgagaa gaaatagggc gaagttcaca acggcagcgc
    18661 gaagttcaac ggcgcatcta cccttccccc cgctgcagca ccgcgaggaa cgcctcctgg
    18721 ggaatctcca ccgccccgac ctgcttcatg cgcttttttc cttccttctg cttctcgagc
    18781 agcttgcgct tgcgcgtgac gtcgccgccg tagcacttcg ccagcacgtt cttgcgcagc
    18841 gccttcacgt tctcgcgggc gatgatgtgg ccgccgatcg ccgcctgcac cgccacatcg
    18901 aacatttggc ggggaattaa tgatctcaat ttgacaacca gttcacgtcc acgctgctgc
    18961 gcgcccgagc ggtgcgccat gctcgagagc gcgtcgacgc gttcgccgtt caccaggatg
    19021 tcgagccgca ccatgtcggc ggcgcggtac tccttgaact cgtagtcgag cgagccgtag
    19081 ccgcgggtca tcgacttcag gcggtcgaag aagtcgagca ccacctcgtt cagcggcagg
    19141 tcgtaggaga tcaccacgtg gcgcgccgag tagtgcatgt tggtctgcgt gccgcgcttg
    19201 tcggtgcaca gcgtgatcac cggcccgacg tattcctgcg gcacgaagat cttgcacgcc
    19261 atgatcggct cgcggaactc ctcgatcgtc tcgggcgggg gcaggcgcga cgggttcgag
    19321 agctgctcga gggttccgtc gcgcagcttc acctcgtaga ccaccgacgg cgcggtggtg
    19381 atgaggctct ggccgtactc gcgctcgagg cgctcctgca cgatgtccat gtgcagcagc
    19441 ccgaggaaac cgcagcggaa cccgaagccg agcgcctgcg aggtctcggg ctcgtagtgg
    19501 aacgaggcgt cgttcaggtg cagcttctcg agcgcctcgc gcagcgactc gtattcgttc
    19561 gcctcgaccg ggtacaagcc ggcgaatacc tgcggcttga tttccttgaa gccgggcagc
    19621 ggcctggcgg cgggcttgac cgcggtcgtg atcgtgtcgc cgaccttggc ggcgcgcagc
    19681 tccttcacgc cggcggtgac gaagcccacc tcgcccgcct tgaggtcttc cttgaatttc
    19741 gacttgggcg tgaaaacgcc gacctgatcg cacaaatacg agccgccggt gctcatcagc
    19801 aggatcttct ccttcggccg gatcgtccct tccatgaccc gcaccagcat caccacgccg
    19861 acgtagttgt cgaaccagga gtcgatgatc agggcttgca gcgcgttcgc cgggtcgccc
    19921 gccggggccg gcacccgtgc gatcaccgcc tcgaggatcg cgtcgacgtt ctcgccggtc
    19981 ttggcgctgg cgcggatcgc gtcctgggcc tggatgccga tcacgtcctc gatctcctgg
    20041 atcgccttct ccgggtcggc cgaaggcagg tcgatcttgt tgagcaccgg gatcacctcg
    20101 accccctgct cggtggcggt gtagcagttc gccacggtct gcgcttccac gccctgggag
    20161 gcgtcgacca ccagcaccgc gccctcgcag gccgcgagcg agcggctcac ctcgtaggag
    20221 aagtcgacat ggcccggcgt gtcgatcagg ttgagcaggt aggtctcgcc atccttggcg
    20281 cgatactcca gcgccgccgt ttgcgccttg atggtgatgc cgcgctcgcg ctcgagctcc
    20341 atggtgtcga gcacctgctc ggccatctcg cgctcggcga gcccgccgca catctggatg
    20401 aagcggtcgg cgagcgtcga cttgccgtgg tcgatgtgcg cgatgatcga gaagttgcgg
    20461 atgcgactct gggccataga aaagcccgcg attctagcgc agcttggcga tcgcctcggg
    20521 agtcaggcga tgcttgagaa cgacatcctt gtaaacgagg accggcaggt cgcggccgta
    20581 ctgcagacgc aggtgcggat cggtgtcgac gtcgatttcg tcgaaggcga tgccgagcgc
    20641 acgcagcccg gctgccatct cctcgcagag atggcagccg ctgcggctgt acaacctagc
    20701 ccttgtcagt gaggcccgag atggtgacga acgcggtgct ctcgccgcgg cggacctgca
    20761 gggtgatgac ggcgttcttg tcgagcccgg cgagcagctt gttgaattgc tcgacggtct
    20821 tgagctcggt gtgctgtccc ttgtgcacca tggtgagcaa cacgtcgccc ttgcgcacgt
    20881 cggccttcga gtctggccgc acgtcggtca ccaccaggcc gccggtcacg ccgagcccct
    20941 tcctctgctc ggcggagagc tcgccgacca cgatgccgag gcggttcgcc ggcgcctcgg
    21001 cctggggctt gctgcgcccc ggcgtggtgc tggaggcgac gcggtcttcc tgcagctcgg
    21061 cgaccgtgat gttgagcttc tgcgtcttgc ccctgcgcca aacttccagc gccgactgcg
    21121 aacccggccg ggtcgagccg acgatgcgcg gcaggtcgct cgagctctcg acctgcttgc
    21181 tgtcgaagga aaggatgatg tcggtcgcct cgatgcccgc cttgtccgcg gggctgccct
    21241 tctccaccga gttcaccagc gcgccgcgcg gccggtcgag gccgaacgaa gtggcgaggt
    21301 cgcgcgtcac ctcctggatc acgacgccga tgcggccgcg ggaaacccgg cccttctcgc
    21361 gcagctgctt ctggatgtcg agcgcgacgt cgatcgggat cgcgaacgac agccccatga
    21421 agccgccggt gcggctgtag atctgcgagt tgattccgac cacctcgccg cgcatgttga
    21481 acagcggccc gcccgagttg cccgggttga tcgcgacgtc ggtctggatg aagggcacga
    21541 agttctcctg cggcagcgag cggcccttcg cgctcacgat gcccgcggtg accgtgttct
    21601 cgaagccgaa cggcgagccg atcgccacca cccattcgcc gaccttgagc ttggtcgggt
    21661 cgccgaactt caccgcgggc agcgatgcgg cctcgatctt gatcaccgcg atgtccgtgc
    21721 gccggtcggc gccgatcacc ttcgccttga actcgcgctt gtcggtgaag cgcaccgtga
    21781 tctcgtccgc gccttccacc acgtgcgcgt tggtgaggat gtagccgtcg gcgctgatga
    21841 tgaaccccga gccgagcgag cgcgcctcgg gctggcgcgg gccctggccg ggcccgggtt
    21901 gctggcgcgg gatgaagcgg cggaagaact cgagcacttc ctcgtcctcg atccccggca
    21961 cctggggcgc ggcgccgctg cgacgcaccg cctgcgtggt gctgatgttc acgactgcat
    22021 tgccctggtc gtcgaccagg cgggtgaagt cgggcagctc gcgcaccgcc tgggacaatg
    22081 cgggcgcggg cgcggcggcg cagaagacgg ccagccagag ggtgacgacg gctctcactg
    22141 cgttcatctt aatctcccaa agactactgc ggccggcgat attcgacggc gttcgcaatg
    22201 cgctgcacgc tcgccgccgg cgcctcgccg accacggtca ccatgtgatt ggcgacctcg
    22261 cgcgtataga tgtggatcgc gcccatgctg gaaagcccgg tgcgcaccgg gtcgctgcgt
    22321 ccctcgagcg attcgatgaa caccgagacc gccgccaggc cgtccgagta cacgacctgc
    22381 ccggccgtac gcgattcgcc aagccttcgt ttcaattcaa cgattttttg aaaaccggga
    22441 agctcgctcg agaggcccca gccggccagg cgcgccggcg cggcttccgt gtcctcggtg
    22501 cgccagctgc gcgaggcatg gcgcgacttc accatctccc ggctcacgcc cccgatggtg
    22561 agctgggtga acgtgaactg ctcgacctgc tcgccggcgg catcgacggt caccgccctg
    22621 aggagcatcc cgctattggt gtcggcgtag agccggtagc cgtagcgcag gttgtcccgg
    22681 ggcgtgagca cgaccgcctg gcagtcgaag ttggcgatgc ggcgcgtctc gccaaggctg
    22741 atgtcgtagt ggcgcgcgag cgccgtgatg cgctcaggca ggagcgccgg gaaatcgcgc
    22801 tccgtgcgcc ggtcgacctt caccaggcgc aggcccggca ggtagcagcg caccgtgtcc
    22861 ttggtgcgca cgatctcgcg cggcatcccg tccaggactt cgagcttctc gatgtcgccc
    22921 gaggcgtcga cgtagcgcgt gatgcgtgag gtctcgctgc gggggccgtt ctggtagacg
    22981 aaggtcccgg tgtaggaaag cttctgggtg gcgtcgtgca tcttgcgcag ccagccgagg
    23041 gtttcgggcg actgggcctg cgccgctccc gagcccaggg cgaccgcgat cagaaaagcc
    23101 cgcagcatta ccggcgcggc tccacggcct gctcggagac cgtgcgcacg tacggcacca
    23161 tgccctgcag cgacacccgt ggcgagaagc cctgatgcgc caacagatag tcattcgccg
    23221 cggtgggcag cgggacgatg ttgggcttgg cctcaggctg ggcctgcgcc accggcgttg
    23281 gcccgggcgc aacctggggc tgcggcgcga acgccatcca cccgaccagc gccacggctg
    23341 cgaggctggc ggccgcggaa agcgcgaacc acctgcgggg ttcagcaggc agcgcgcgcg
    23401 gcgcgagcac cgtcggctcg gcggccaggc gcgcggaaag ccgcgcggcg aagcccccgg
    23461 agagcggccg tcccccgcgc atcgagtcgc cgatcatgtg gtaggtaccc caggcttggc
    23521 gcgcctcgcg gtcgcgtccc agcgcttcga gcactgcggc ggcggaccgg tcgtccagct
    23581 cgccgtccat caactccgag agcctttcct tcatacccct accacctctg gtctttccgt
    23641 gtatccaaca gcggcctgag ctgctccgcg atcgcctcgc gcgcccgaaa gatcctcgag
    23701 cgaacggtcc cgatcgggca attcatcgcc tgcgcgatat cttcgtagct catcccttca
    23761 atctcgcgca tctgaatcgc cctgcgcagc tcctcgggca aaccctcgat cgtggaattc
    23821 accgtctccg cgatctcgtt cgacagcagc acgctctccg gcgtgttgat gtcgcgcaac
    23881 tgctcgccct cctcgaaccc ttccgcctct tcggcctcta cctcggtcga ggtcggcgcc
    23941 cgccgaccca tggccatgag atagtttttg gccgtattaa ttccaatccg gtaaagccac
    24001 gtatagaacg cggagtcgcc tcggaacgcg gggagggccc tataagcctt gatgaacgcc
    24061 tcctgcgtca cgtcctcgac ctctgccggg tcgcggatga agcgtgacag caggcgcgcc
    24121 agcttgcgct ggtacttttc gacaagcagc tcgaaggcct gcttgtcgcc gccttgcgcc
    24181 cttgcaacca gctgacggtc gatctcccgg tcagtcatgc aacctgcacc tgaccggaac
    24241 ttcgatcggc aacggcacca gggacgcgca gcgagggctc cccacccccg gactcacatc
    24301 cggtttccta cgcctatgtg ccacagacac acgcactttg aaatagttcg aagccttcct
    24361 cgcgtccgag tcaggccggc agttgtttgg ccgccacggg cagcctgccc cagagcgccc
    24421 agaggcgaag tcggcggaac tcctcggcgc cgagcatgtc gcgcgcgacc aggatggtgc
    24481 ggcgcatcgg gcgccgcaac ggcagcgtca ccatcattcg gctgacgtgg cgggccgggc
    24541 cgagctcggc ctggacccgg cggccatctt tcagctcgag gagaatttca ttccttgaca
    24601 attccagggc tcggaccgac gccttcgatc catggagcgc gcgagcccag gcggccgcca
    24661 tgccgagcgc caggaaagcc gcgcccagcg cgtagccgag agcagttccc atcaccgtga
    24721 tcaccgatgc cgctgcggcg gcgtgcaacg ccaggatcgc gccggccaga ccccaggagc
    24781 gggaaagctc caggcgcgtg ccgctcaagg ccgcgccata acgagggtcg cgttggtgcc
    24841 gccgaagccg aacgagttgg agagcgcgac gcgaatcggc atcttgcgcg cgacgttcgg
    24901 aacgtagtcg aggtcgcact cggggtcggg cgtgcgcagg ttgatggtcg gcggcgagac
    24961 ctggtcgcgg atggcgagcg cggtgaacac cgcttcgata ccgccggcgg cgccgagcag
    25021 gtgtccggtc atcgatttgg tcgagttcac ggcgacgctt ccggcatgcg cgcccagcac
    25081 gcgcttcacc gccttggtct cggcgatgtc gccgagcggt gtggaggtgc cgtgcgcgtt
    25141 gatgtagtcg atggcgctgg gctcgattgc ggcgtctttc atggcgttgc gcatgcagcg
    25201 gaacgcgccg tcgccgtcct cgctgggcgc ggtcatgtgg tacgcgtcgg ccgacaggcc
    25261 gtagccgatg agctcgcagt agatgtgcgc gccgcgcgct ttcgcctgct cgtactcctc
    25321 gagcaccacc gcgcccgcgc cctcgccgag cacgaagccg tcgcgctcct tgtcccacgg
    25381 ccggctcgcg gtcgtgggat cgtcattgcg cgtcgagagg gcgcgcgccg aggcgaagcc
    25441 gccgacggcg agctcggtca ccaccgcctc cgagccgccg gcgatcatgg tgtcggcctc
    25501 gccgtagcgg atcgacttca tcgcctcgcc gatgcaatgg gtcgaggtgg tgcaggcggt
    25561 caccatcgcc acgttcgggc ccttggcgcc gatctcgatc gacaggagcc ccgcggtcat
    25621 gttgatgatg ctgccgggaa tgaagaacgg cgagagacgc cgcggtccct tctgttcgag
    25681 ctcgtggtgc tgcgcctcga tcagcggcag gccgccgatg ccggagccga aattgatgcc
    25741 gatgcgctct gcgttctgcg gcgtcacctg cagcccggcc tcgcgccacg cctgcagccc
    25801 cgcagccatg ccgtagtgga tgaagacatc catgcgccgc gcttccttcg gcgacatgta
    25861 cggcgcgacg tcgaagccct tcacctcgcc cgcgatctgg ctcgcgaggc gcgaaggatc
    25921 gaagcgcgtg atgcgggtga tgccggattt cccggcgagg gcgctggccc aggcgtcagc
    25981 gacggtattt ccgatcgggc tgacgattcc cagcccggtg accacgactc tgcgtcgatt
    26041 cacttacgac tttgcgtggg ccttgatgta gtccacggct tgctgcacgg tggtgatctt
    26101 ctccgcctcc tcgtccggga tctcggtctc gaactcctcc tcgagcgcca tcaccagctc
    26161 caccgtgtcg agcgaatcgg cgcccaggtc gtccacgaac gacgaatcgt tcttgatctc
    26221 gccctcgttc acgccgagct gctcggcgat gatctttctc actctttgtt cgacgttttc
    26281 catcgcggct ccttcccgaa gagggggcgt attttacgac atgtacatgc cgccgttgac
    26341 gtgcaggacc gcgcccgtca cgtagccgcc gccgggcgag gctagatagg ccaccgcggc
    26401 ggcgacgtcc tgcgcggagc ccaggcggcc cagcgggatg ttctcgagca ggaccttgcg
    26461 ctgctcgtca gacagcgcgc gcgtcatgtc ggtgtcgatg aagcccggcg cgacgcagtt
    26521 gaccgtgatg ttgcggctgc cgagctcccg cgcgagcgat ttcgtcattc ccaccacgcc
    26581 cgcctttgcc gcggcgtagt tcgcctgccc ggcattgccg gcggcgccca cgaccgacgt
    26641 aatgttgatg atccggcccc agcgcgcctt gatcatcgcg cgcatcacct tgcgcgacag
    26701 aagccagacg gcgcgcaggt tggtctcgat gacctcgtcc cagtccgcgt ctttcatgcg
    26761 cagcgccagg ttgtcgcgcg tgatcccggc gttgttcacc aggatcgcga tgtcgccgac
    26821 ttccttctgg atcgactcga cgagcgcgtc gcactgggcc gcgtcgcgga cgttcaggac
    26881 ccgcccgttt atcggggcgt ttatcttttt ggcgccctcc gccgtcgtgg aagtgccgat
    26941 cacccgggcg ccctgccggc cgagctcttc cgcgatcgca tggccgatgc cgcgcgacgc
    27001 acccgtcacc aacgcgattt ttccttctaa catttcaagg cctcttcgag agaagcgcga
    27061 tccgcggcag cgagcgactt gacccccggc gcgatgcgct tggtcatgcc cgagagcacc
    27121 ttgcccgggc cgcattcgac gatgtgcgtg acgcccgaat ccgccattcg gcggatggtc
    27181 tccacccagc gcaccggcga tgccgcctgg cgcacgagcg cctgtcggat ttttccgggc
    27241 tcgttttcca cggcgacgtc gacgttgttc accacggcga tcttcggcgc cgtcagcgct
    27301 gccttttcca ggtaagtctt cagcttgtcg gccgcgggcc gcatcagcgt cgagtggaac
    27361 ggcgcgctca ccggcagcag catcgcccgc ttggcgccct tcgccttgca gatctcgatc
    27421 gcgcgctcga ccgccgcttt ctctccggcg atgacgacct ggccgggcgc attgaagttc
    27481 accgcctgca ccgcgccgcc ggcctgcgcg caggcggcgc gcaccgcgtc gtcgtcgagc
    27541 atcaggatcg ccgccatcgc tccctgccct tcgggaaccg cttcctgcat cgactgcgcg
    27601 cgaaagcgca cgagcggcag gcagtccttg aagctcaacg cgcccgcggc gaccaaagcg
    27661 gtgtattcgc caagactgtg tccggccacg aactcgggtt gcggaccgcc aaggccgcgc
    27721 caggcgcgat acgccgcgta gcccgcggtc accatcgccg gctgcgtatt gacggtcagg
    27781 gtcagcttct ccgccgggcc ttcgtccagc agcttcacga agtcttcgcc cagcgcggtc
    27841 cttgcctctg ccacgacccc ctcgatttcg ggaaggcccg cgtaggcctt cagcatcccc
    27901 accgcctgcg acccctggcc gggaaagacc atggcgagct tcacatcgtc accaggctcg
    27961 agccccaggt gaagccgccg cccacgcctt gcaacagcac gcggtggccg ggccggatct
    28021 tgccggcgcg cacgaattcg tccagcgcga gcggcaccga agccgcggag gtgttgccgt
    28081 ggtggtccac cgtcaccacc agcttcgccg gcggcaggcc gagcttgcgc gccgtggcgt
    28141 cgagaatgcg cacgttcgcc tggtgcggga tcagccaatc gacgtcggcg agcttcaggc
    28201 cggcggcggc gacggtctcg cgcgccgact cgtccagcac tttcaccgcg aacttgaaca
    28261 ccgcctgtcc gttcatctgc aggaacggcg agccgatcac ggtgccgccg cacacgttcc
    28321 cgggcaccga gagcatcccc gcctggctgc cgtcggctcg cagcacgctg gcatggattc
    28381 cagggttttc ggaagacacc aaaaccactg cgccggcacc gtcgccaaag aggacgcacg
    28441 tgccgcggtc gttccagtcg aggatgcgcg agaagacctc cgcgcccacc accagggctt
    28501 tcctgtattg accagacttt atgaagctgt ccgcggtggc aagcccgtag acgaacccgc
    28561 tgcacactgc ctgcacgtcg aacgccgcgc tgcccttgac gccgagcttt gcctgcagca
    28621 ggcacgcggt gctcgggaac acgtagtccg gcgtggacgt cgccaccacg atcaaatcga
    28681 tttcacttgc agaaacacct gaagattcca aagcgcgccg gctcgcctcc agcgccaggt
    28741 cgctcgacgc ctgcgacttg tcggcgatgt ggcgctggac gatgccggtg cgctcgcgga
    28801 tccaggcatc gctcgtgtcg agccgcgccg cgaactcggc gttggtcatg acgcgcggcg
    28861 gtagatagct gccggtaccg gcaatgcgcg aatagatcat tgaaccggtt gcggttgcag
    28921 ctggctcatg cgctctgcga tgcgctgcgg cacgttgttg cgcacctcct cgaccgcgcg
    28981 ctcgagcgcc tggccgaagg caaacacgtc ggccgagccg tggctcttga tgacgatgcc
    29041 gcgcaggccg agcaggctcg cgccgttgta gcgccgggga tcgagccgct cgcggaaggc
    29101 gcggatcacc ggcatcgcga ccagcgcggc cagcttggtg agcgggttgc gcgagaactc
    29161 ttcgcgcatg gtcgtggtca tcatctgcgc cacgccctcc gaggccttga gcgcgacgtt
    29221 gccgacgaag ccgtcgcaca ccacgacgtc gaccgtgccc ttgtagatgt cgtcgccttc
    29281 cacgttgccg acgaaattga gcccgctccc gcgcagcagc accgccgcct gcttgaccgc
    29341 ctcgttgccc ttgatgtcct cgacgccgat gttgagcagg ccgaccgagg gccgctcctt
    29401 gtgctccacc gccgcgacca gcatcgcgcc catgatggcg aactgcagca ggtgctcggc
    29461 gccgcagtcc acgttggcgc cgagatcgag cacgtaggtg ctggtgcgcc gctggttggg
    29521 aagcacggtc gcgattgccg ggcggtcgat cgcaggcagg gtcttcagca cgaagcgcga
    29581 gatcgccatc agcgcgccgg tgttgccggc gctcacgcag gcgtgcgcct cgccgctctt
    29641 caccaggttg accgccacgc gcatcgacga atccttcttg tagcgcagcg cctgcgccgg
    29701 gggctcgtcc atcgcgacga cctcgctggc ggggtagacg cgcacgcgcg ggttcgttcc
    29761 ggaaaggtgg ctttcgattg cgtcgcgctg gccgaccagg accacctcga cgtcgggcgt
    29821 ccgcgcctga aactcgagcg cggccggaac cgtcacatgg gcgccgtggt cgccgcccat
    29881 gcaatcgacc gcaaccgtga tcaacgcggc tactcttcct gcgtcttgac gaccttcttg
    29941 ccgcgataga acccggtcgg gctgacgtgg tggcgcaggt gcgtctcgcc gctggtcggc
    30001 tcgacggcga tcgcgggcgc gctcaggtga tcgtgcgagc ggcgcatgtt gcgccgggac
    30061 ggcgacttct tgttctggtg gactgccatg tgtgcgactc ctctctaatc aatgtttgct
    30121 tatcaggccg cgcagcccgg cgaacggcga agtcttctgc gcgccgtcgc tcgccgcggc
    30181 cggagcgcaa tcctcgtgcc gcggcgcgta cggcaccgcg aggatcagct cgtcctcgat
    30241 cagctcgcgc agcgccatct ccttgcccgc caccacccgg tccggcgcac gcgcgtcggc
    30301 gggctcggcg tggatctcgg caagcgtcgc cgccagcacg agcgtctcgt ccgtatgcac
    30361 ttcgagcgcc atcgcttcca ggcagcgctg acagcgaagc gcgagcgtac cgctgaccct
    30421 gatccgcagg cccggccgcc cgcgctcgtc gcgcactccg cggatctcgt acctgacttc
    30481 gccgccatcc gccgcgagca tgtcgcgcag gcgcggaaag tcgctcagcg gccaaattcc
    30541 ctgctgcgtt gcgccggcgg aagcgaactc gaagccgtcg ataaccggtt gatgcgccgg
    30601 cgggtgcgac ataaacgcgc gataatatca tcgcctcaaa agctgcgtca aaacaaagtg
    30661 atgcctcaac cccggcgact cgtgctcgcg tccggttcgc gctaccggaa agcgctgctc
    30721 gaacggctcg gattgccgtt cgaagccgcc tctccggata tcgacgaaac gcctctaagc
    30781 ggcgaatcgc ccccggacac cgcgcttcgc ctggcgcatc tcaaggcaat ggcgctttcg
    30841 caccgcggcg acctcatcat cggcagcgac caagtggctg cgagcggcgg acggcgctac
    30901 ggcaagcccg gcggccatga aaaagccgtt cgccagcttc gcgagctctc cggcaaagcc
    30961 gtggactttc acaccgccgt cacgctcctg gatacccgtc gcgacaagca cgagtcgcgc
    31021 ctcgtgccgt gccgcgtggt cttccgcacg ctcgacgagc gccgcatcca ggcctacctg
    31081 gaaagagaga agccctacga ctgctccgcc agcgccaagg ccgaaggcct ggggatcgcg
    31141 ctgatcgagc gcatcgagac cgacgatccg agctccctca tcggcctgcc gctcatcgcc
    31201 ctgagcgaga tgctcgagcg cgccggcctg cccgtcctgt aaagcccatc ccaaaacagg
    31261 gacagaccac gattaactcg ccggtcctat acgcaatccc gacgtcactc ggtggaccac
    31321 ccgagaaggc attgccccag gatgtcgtcc ggcaggtgca ggctttacgc gacttcgtcg
    31381 tcgaaaacgc aaagagcgcg cgtgcatttc ttggtgcgct cgggatgccg gtacgggaac
    31441 tggatatcag ggtcatcggc gaggaacgcg atcctctggc gcctttgagg gaaaagcgct
    31501 cgctgggctt gctgtccgaa gccggctgcc cggcgatcgc cgatcccggc gcggcgattg
    31561 tcgaggctgc ccaccgcgaa ggctttcgcg tcgtaccgct ggtgggcccc tcctccatcg
    31621 ttctcgcgct gatggcttcg ggcctcgaag gccagcgttt tgccttctgc ggctacctgc
    31681 cgcgagacga ttctcagcgg aagcaaagga tccgcgaact cgagcagcgc tccaggaaag
    31741 aacaggaaac ggagatcttc atcgagacgc cgtaccgcaa cgacgtcctg ctcgccgcgc
    31801 tcgtcgaaac ctgtaatacc ggcacgaggc tatgcgttgc cgccgacctc accctcccta
    31861 gcgaaagcgt tcagacgaag ccgatcggag agtggcgccg ggcgaaggcc gcgatcggcc
    31921 gccgcccggc ggtgtttctc ctgctagcgg attgaagtgc gcaacagggc ctcgacgaag
    31981 gcgctgctga ccgaagcgcc gaagcgccgc gcgagcttct cggcgacgcc ctcgctgatc
    32041 gtgaagtcgc ggatctcctc ggccttgatc acctcgcgcg cgacgtactc gaggctgccg
    32101 aagccgtcgg cgagcccgag gtcgacgctc ttctgcccgg tccagatgag gccgctgaag
    32161 atctcgggcc cttccttgag gcgcttgccg cgcccctcgc gcaccacgcc gatgaactgc
    32221 tggtggatgt cgcccatcag cgtcttgatg tgctgcttgt ccttgtcgtc gagcggcgag
    32281 aacgggtcga gcatgccctt gttctcgccg gcggtgatca gccgccgctc gatgccgagc
    32341 ttgtccatga ggccggtaaa gccgaagccg ttcatcagca cgccgatcga gccgacgatg
    32401 ctggctttcc ctacatagat gcggtcggcg cccaccgcga caaagtagcc gcccgaagcg
    32461 cacacgtcct cgaccaccgc atagagcggg atctcggggt acttgctgcg cagacgcttc
    32521 atctcgtcgt agatcgtttg cgcctgcacc gggctgccgc ccggcgagtt gatgcgcacg
    32581 atcacgccct gggtgttttt gtccttgaag gccgcctgca gcgcggtggt gatcttctcg
    32641 gcgctcgcgt cggtgccggg cgcgatcagg ccgctcacct cgaccatcgc ggtgtgcttc
    32701 ttgctgccgc cgacgtcgcc gcccttccag tcgatcgcca tcagcaggaa caccgtgatg
    32761 taggcaaagg tcagcagctt gaagaagatg ccccaaaggc gcgcgcgccg ctgctccttg
    32821 agcgccgccg tggcgagctt gttgaccagc tcgcgttccc actgaccacc gttctcagtc
    32881 atcgtcgaat cctatgaagt agaccaggcc gttacgttcc tcgaggcgca gcttcacgag
    32941 cgggctgccg ttgcacgggc cgcccaggca gcggccggtg gcggcgtcgt aggtggcgcc
    33001 gtgcgtcgag catagcaaat cgcgtccgtc ggaatcgaaa aatacccctt cctgccagtc
    33061 gagttccatc gcgacgtgcg cgcagcggtt cagataggca tggactttcc catcctttcg
    33121 aacggcaaag gccggggccg cctcgccgaa gtactcgacc tcgaagcgca cgccgcgccc
    33181 ggcgtcgatc agcacgcccg actcgcagat caggcgttcg ctcgcagcca ttcttccaat
    33241 tgcgtgactg aagagaagca gccgagcggt tcgcaggcgc gcaggctgtc ctccggatgc
    33301 gcgccgtagg tcacggcgag cgcatccacc ccggcggcgc gggccatttc caggtcgtgc
    33361 gaggtgtctc cgatcatcag ggaggaatct ttcggcaccg cgagctcttc gatgatctcc
    33421 ctcagcatgg ccgggtgggg cttcgggttg gtctcgtcgg cgcagcgcga cgcggcgaac
    33481 agcggcttca gccggtggaa ctcgaggtcg cggtcgaggc cgcggcggct ttttccggtg
    33541 gcaatggcga gaacatgcct tttcgaaaga tcttcgagca gcggacgcat gccgtcgaaa
    33601 agctgcatgg cgtccttgcg cacgaggaaa tgcttgcggt agctcgcgac gaattcggga
    33661 tagcgctcgg ccgccagccc ggggacggcg atgcgcatgg catcgtgcag gcccaggccg
    33721 atgacgtggc tggcgcgctg ccagtcgggc acttccaacc cgagctcgcg gctcgcgtcg
    33781 cggatgcaat cggcgatcgt cgccgccgag tcgatgatcg tgccgtccca gtcgaagaca
    33841 agcagccggt agctcacggc agcattttct ccaggaaagc cttcatctcc gcaggcaacg
    33901 gagactcgag cttcaccggc tcgccgccga gcgggtggcg gaacgagagc ttcgccgcgt
    33961 gcaggagcag ccgttgcttg agcgcccggt tgagggcgaa atcgccgtac ttgtcgtcgc
    34021 cgacgaccgg atgcccggcg tgcgccaggt gcacgcggat ctggtgcgtg cgcccggtca
    34081 tgagctcgac ttggaggagg ctgaactcgc cgctgctctt cacccgcgtg accctcgtca
    34141 acgcggacat gccgtccgcc tgcacgctga cccgacgctc gccggaggcg gtgacgtgct
    34201 tgtgcaggga ttcgcgcagc tcgagggtct tcttggccag ggcgcccttg acgattgcca
    34261 ggtagatctt ccggacctcg ccctcgcgca gcatgcgatg caactcgacc agggcgctcc
    34321 gcctcttaca aagaatcaat aggcctgacg tgtcgcgatc caggcgatgc gcgagctcga
    34381 ggaccttggc ctgcggccgc gccgcgcgca gcgactcgat cacgccgaag ctcactccgc
    34441 tgccgccgtg cacggcgatg cccgagggct tgtcgatcac caggagggaa gcgtcctcga
    34501 acaccaccgg aaggtcgagc ggacgcgccg gcgggggtct tccttcggaa accctgaccg
    34561 gcgggatccg gaccctatcg ccgacttgca gccggtagtc gggcttcacc cggccgctgt
    34621 tgacgcgcac ttggccgctg cgcagcacgc ggtagacatg gctcttcggc acgcctttca
    34681 gctggcgcag caggaagttg tcgatgcgct gcgcctcgct ttcctcgccg atcaccagcc
    34741 acttcgcctt aggtcccgcg gtcttgctta agtcattcat tttccgtatg atcttttcac
    34801 cgcttcgagt tgcgaggcac aggaccgctt tggggattca acctccacca gcgcctgcaa
    34861 cccgcctcct gcccggccgc agccgagcct gaagcgctgg ttaatttcgc tcaccggaag
    34921 tagcgatact actagcagcg cgcgtagggg ctgttttccc actgtatcca tgaggagacc
    34981 ttcgaggagg ttgcagctcg ggtaggtagg cctctcctgc gcgcgcagga gaacaacaag
    35041 tgaagcgcat gttgtttaac gcgacgcagc aagaggagct gcgcgtcgcg atcgtcgacg
    35101 gacaaaagct cgtcgacctc gacatagagt cggcatcaag ggagcaacgc aagagcaaca
    35161 tctacaaagg catcatcacc cgcatcgagc ccagcctcga ggctgccttc gtcgactacg
    35221 gcgaggaacg gcacgggttc ctgccgttca aggaagtttc ccgcagctat ttcaaggacg
    35281 gaatcgacgc cgggcgcgcc cgcatccagg acgccctgcg cgaaggccag gagttgatcg
    35341 tccaggtcga gaaggacgag cgcggaacca agggcgccgc cctcacaacg ttcataagtc
    35401 tggcagggcg gtatctggtg ctgatgccga acaacccgcg cggtggcggc gtctcgcgcc
    35461 gggtcgaagg cgaagaccgc aacgagcttc gcgacaccct cgaccagctc cagcttcccg
    35521 aaggcatgag cgtcatcgcc cgcaccgcgg cgatcggccg ctcgctggag gagctgacct
    35581 gggacctgaa ctacctgatg cagctctgga ccgcggtcga gggcgcctcg gcggaccagt
    35641 cgaagaagcc gttcctcatc tacctcgagt cgagcctggt gatccgcgcg atccgcgatt
    35701 acttccagcc cgacatcggc gagatcctga tcgacacgga agagattttc gagcaggcca
    35761 agtccttcat gcagacggtg atgccggaca acgtcggcaa ggtgaagctc taccgcgacg
    35821 acatcccgct cttctcgcgc ttccagatcg agcaccagat cgagagcgcc tactcgcgcg
    35881 gggtcgccct gcctgcgggc ggcgcgatcg tcatcgacca caccgaggcg ctggtcgcca
    35941 tcgacgtcaa ctccgcgcgc gccacgcgcg gcggcgacat cgaggaaaca gcgttccgca
    36001 ccaatctcga ggcggcggag gaagtcgcgc gccagttgcg gctgcgcgac ctgggcgggc
    36061 taatagtgat cgatttcatc gacatggagt cccagaaaaa ccagcgcgag gtcgagaacc
    36121 ggctgcgcga ctcgctgcgg tacgaccgcg cccgggtcca aatcggcaag atctcgcgct
    36181 tcgggctgat ggagctctcg cgccagcgtc tgcaggcctc gctcgaggag accgcgcaca
    36241 tcagctgccc gcgctgcagc ggcaccggct tcatccgcgg caccgagtcg accgcgctcc
    36301 acgtgctgag gatcatccag gaagagtcga tgaaggagaa caccggggcg gtgcacgccc
    36361 aggtgccggt cgatgtgtcc tcgttcctgc tgaacgagaa gcgcgccgag atccagaagc
    36421 tcgaggcccg gctgaaggtg aacatcgttc tcgtgcctaa tccgcacctc gagacgccgc
    36481 actacaaggt gcagcgcctc aagcacgacg agctgaacgc catggagcac gtgccgtcga
    36541 gctacgagct ggtcgagcgc cccgaggagc ccaaggcgcc gggcgccgag gaggaagtga
    36601 agctcgagcg ccagcagccg gtggtgaaaa ccatcacccc ggaccagccc gcgccgctcc
    36661 cgcaggcagc cgctccgcag ccagcgccgc gtccggcgca acccgcttca cagccggccc
    36721 caatgcccgc agcaccgcaa ccccagcctg cctccggcgg cggattcttg agccgactct
    36781 tcggcgggct gttcggggcc gagccggcgc ccgcgcccac ccccgtcgcc gcctcacccg
    36841 cgccgcaggc ctcgcagccc gcgcaacgcg agcccaggcg cgatcatcat cgccgcgatg
    36901 gacgcaggga cggcggccgc ggccgcgatg gccagagaga cggccggcgc gacgagcagc
    36961 agcgtgaccg ccagcaacac ccgcggctcg agcaaaggcg caacggccag cagccgcgcg
    37021 gtgatcgcgg cgagcgggat cgcggcggtc gcggcgagcg cggccggccg gaccaccagc
    37081 agcgtcagca acgcccgcag catcaagggc cgcagcagca gggacagcag ccgcaacagc
    37141 aggaacaaca gccgccgccg caggaacaac agccgcagcc gcagggacaa caaccccagc
    37201 agcagcaccg gcctccgcca cgcgagcgcg aggcgcgcga aggccgagag ggtgaagagc
    37261 agcagcgccg cggcggccgc cgcaaccggg ggggacgcga ccgacagcgc gagcgcgggg
    37321 atcgcggcga gcgcccggcc cagggcgatc cgcggccgtc aggcgcaccg ccttcgttcc
    37381 tgatgaaaga ggatcaattg cgcgaagacc agcggcgcga agcgctggcg cgcaaggagc
    37441 tcgagtcctc cggcgaggag accttgccgc catcggtcgc accgcgcgtg gaagaacccg
    37501 cgccacgcat ggaacaggcg gcgccacgca tggaacagcc ggcgccgcac gcggagcaac
    37561 cggtccgcgc ggagccgaag atcgactcca aggtcttgct cgaaagcgcg gggctggtga
    37621 tgatcgaaac cgaccgtgca agggtacccg ccgctgcgcc cgcccaggag gagcagcagc
    37681 atctcggccg gccccgccgc gagcgccaga aaccggtggt ccaggacgag ccgttgcagc
    37741 aggtggagac gactaggaag taggctcttt caactctgca aggaggctgg cgcactgggc
    37801 ctccaccagg tcgagcacgc gctcgaaccc ggccgggccg ccgtaatagg ggtccggcac
    37861 gtcgctgtcc gccgcgaaca gccggatgtt cgcccgatgc cgcggcggcg ccatgcgctg
    37921 caagtcgcgc aggtggccgc tgtccatcgc caggatgagg tcgaaatcgt cgaagtcgcg
    37981 cggccgcacc tggcgcgcgc gctgcgcgct caggtcgtag ccgcggctcc gggcgtggtg
    38041 ctgggcacgc tcgtcgggcg gatggcccac gtgccagtcg cccaggccgg cggattcgat
    38101 gcgcagttcc agccccgctt tcacacacaa gctcctgaaa accccttcgg ccgtcggcga
    38161 gcggcagatg ttgccggtgc agacgaatag gatggagcgc cgttctttca tatgatcgcc
    38221 attatgactt cctgcgccga cgtcctcgcc ggccgcgcgc cgaaggaggg tgccgtggtc
    38281 ctgaaaggct gggtgcgcac ccggcgcgac tccaaggcgg gcttctcgtt catccacctg
    38341 tcggacggct cgtcgttcca tccggtgcag gtcgtcgcgc cgaaggacct ccccaactac
    38401 gaaaaggaga tcctgcacct caccgcgggc tgcgcgatcg aggccaccgg cggcatcgtg
    38461 ccctcgcccg ccaagggaca gcccttcgag atgcaggcgc gcgcgatcaa ggtcgtcggc
    38521 tgggtcgaag atccggactc ctatccgatc cagcccaagc cgcacacctt cgagttcctg
    38581 cgcgaagtcg cgcacctcag gccgcgcacc aacgtgatcg ctgcggtgac gcgcgtgcgc
    38641 cacaccctcg cgcaggcgat ccaccgcttc tttcacgaac gcggcttctt ctgggtgaac
    38701 accccgatca tcaccgccgc cgacgccgag ggcgcgggcg cgctgttccg cgtttcgacg
    38761 ctggacttcg cgaacctccc ccgggagggg ggaaagatcg acttctcgaa ggacttcttc
    38821 gggcgcgaga ccttcctcac cgtctcgggc cagctcaacg tcgaggcgta ttgcctcgcg
    38881 ctctcgaagg tgtacacctt cggccccacc tttcgcgccg agaactcgaa caccagccgg
    38941 cacctcgcgg aattctggat gatcgagccg gagatcgcgt tcgccgatct ttcggacaac
    39001 gcggatctcg ccgaggcgct gctcaagtcg atcttcaaat ccctcctgca ggaaagaaaa
    39061 gaagacatgg ctttcttcga cgagcgcgtc gagaaaggca tcgtggcgaa gctgcagggc
    39121 atcgtggagt cggaattcgt gcgcatggac tacggcgagg cgatctcgat cctcgagcgc
    39181 gcgaaggaga agttcgagtt ccccgtgaag tggggcgtcg acctgcagtc ggagcacgag
    39241 cgctacctca cggagaagca cgcgaagaag ccggtcgtgg tgatgaacta cccgaaggcc
    39301 atcaaggcgt tctacatgcg cctgaacgac gacgaccgca ctgtcgcggc gatggacgtg
    39361 cttgccccgg gcatcggcga gatcgtcggc ggcagccagc gcgaggagcg cctcgaccgc
    39421 ctggacgccc ggatggcgga agtcggcatc gacaaggaga gcctctcctg gtaccgcgac
    39481 ctgcgccgct acggcacggt gccgcacgcg ggcttcggcc tgggcttcga gcgcaccgtg
    39541 tcctacgcca ccgggctctc caacgtgcgc gacgcgattc cctttccccg gacgccgggc
    39601 aacgcgaagt actgatgcgg atacggcttg ccacgcccgc cgatgcggcg gtgctgatcg
    39661 agttcaactc cgccatggcg ctcgagaccg agcgcaagga tcttctagcg gaggtgatca
    39721 gcgccggcgt ccacggtctt ctgggcagcg ccgctgcggg cttctatgtc gttgcggaaa
    39781 aagacgaccg ggtggtgggc tcgctcctga tcaccaaaga gtggagcgac tggcgaaacg
    39841 gcagcttctg gtggatccag agcgtttacg taaggcccga atggcggcgc caaggcgtct
    39901 acaagcagct ctaccgccac gtccaagagc ttgctgcgca ggacccggca gcgtgcggct
    39961 tccggcttta cgtcgagcgg gagaacagcc gcgcccaggc cacgtaccgg gcgctcggga
    40021 tgaaagagac tcgctacctc gtcttcgagg agctcaagcc cggcgttcgg ttctaccggt
    40081 agaacacgtg gcgcccgatg cgcgccactt tctgcctctc cttggcccac tccggctgga
    40141 tgtagttggc gtggaagtgc agcgcgccgg gcatcaccgc ttgtcgccgc tggtaataca
    40201 cgtcctcggc gaccttgact gcgcgcttcc aggccgtgcc cttgggcgcg tcgagggact
    40261 cgaactcggt ccaggagaat gcgccgacgt agcgcttgcg cagcgggtcc cagttcgtct
    40321 ggtacaccac ctcgcacacg gtcttgggat acaacggcga agccttgcgg ttcatggtga
    40381 cttccgccac cgcatactgg ccggccgggg gctcgccgcg cgcctcgtag tacacgttga
    40441 gcgccaggca ttcgaggctg cgcgcgtgga actcgcgcac cctggcggtc tgctcgtcgc
    40501 ggtgcgcgga gacggcgccg agcgcaagca cgagcacgcc gaccacggcg caaaagaaca
    40561 tcgcgaaggc cagcgcggcc ttgtcggccc gccaccacat caggcgcagc ttcaagaaca
    40621 tgcgcaactt tgccggggcc gcacgcccct gcgaaggtca aattaccttc ttaaggttct
    40681 ttacgctccg cccgaaaaat tcggggacgg agccctaatt cgttagctgc gcgttgccag
    40741 aattagcgct ccgtccccga atttttacgc ttcgacgccg aaaacccgct tcttgagctc
    40801 ggcaagacgg tcgcgcgccg ccgcggcctc ctcgaactcc aggttcctgg cgtgctcgag
    40861 catcttcttc tcgatcgcct tgatctcgcg cgcgatctgc ttttcgctca tcgcttcgta
    40921 gcgcgcctct tcctgcgcga cctgcgcctg cgaggcgtcg tacacgccgt cgatcaggtc
    40981 cttgatgcgc ttgatgatgc ccttgggcgt gatgctgtgg tgcacgttgt aggcgagctg
    41041 cttcgtgcgc cgccggtccg tttccccgat cgccttcttg atggaatcgg tcacccggtc
    41101 ggcatacagg atcgccgtgc cgttgatgtg gcgggcggca cggccgatgg tctggatgag
    41161 agagcgctcc gaacgcagga aaccttcctt gtccgcatcg aggatcgcca ccagggaaac
    41221 ctccggcagg tcgaggccct cgcgcagcag gttgattccc acgagcacgt cgaactcgcc
    41281 caggcgcagg tcgcggatga tctccactcg ttccaccgtc tccacctcgg agtgcaggta
    41341 gcgcaccttg accccatgct cggccaggta ttcggtgagc tcttccgcca ttctcttggt
    41401 cagggtcgtc acaaggattc gttcatgccg tgacaccttg tccctgatct cggacaagag
    41461 atcatccacc tgcgtggagg ccggacgcac gatcacctgc ggatcgacca gcccggtcgg
    41521 gcgcaccacc tgctccacca cctggccgga cttctcgcgc tcgtaatccg ccggggtcgc
    41581 agagacgaac accgtctggc gcaccacttt ctcgaactcg tcgaagcgca gcggccggtt
    41641 gtcgagcgcc gatgggaggc gaaagccgta ctcgaccagg ttctccttgc gcgagcggtc
    41701 gccgcggtac atgccgccga gctgcgggat cgtgacgtgg ctctcgtcga tgatcatcag
    41761 cgcgtcctgc ggcaggtagt cgatgagcgt cggcggcggt tcgcccgcct gacggccgga
    41821 caggtggcgc gaatagttct cgatcccctt gcagaagccg agctcggcga gcatctcgag
    41881 gtcgaagcgc gtgcgctgct cgatccgctg cgcctccacc aggcgcccgg ctttctggaa
    41941 gaagtcgatg cggctgcgca gctccgcctt gatcgcctcg atcgcgcgca gcgtagtctc
    42001 gcgcggcgtg acgtagtggc tcgacgggta caccgtgaag cgcgccatcg ggtggtgcac
    42061 cctgccggtc agcgggtcga acacctccag cttttccacc aggtcatcca gcagagagat
    42121 tcgaatcgcc tgctcggcgt gctccgccgg gaacacgtcg atcacgtcgc cgcgcacacg
    42181 gaaagtgccg cggcggaagt cgagctcgtt ccgctcgtac tgcatcgcca ccaggcgcgc
    42241 gagcacgtcg cgctgcgaca tcttctcgcc ctggcgcagg tgcaggatca tgccgtggta
    42301 ctcctccggg tcgccgatac cgtagatcgc cgataccgtc gccacgatca ccacgtcgcg
    42361 gcgctcgagc agcgacttgg tcgccgacag gcgcatctgc tctatgtgct cgttgatcga
    42421 cgaatccttc tctatgtaga gatcgcgcgc cggcacgtac gcctccggct ggtaatagtc
    42481 gtagtacgag acgaaatact cgaccgcgtt gtcgggaaag aactcgcgga actcggagta
    42541 gagctgcgcg gcgagcgtct tgttcggcgc gagcaccagc gccggtcggc cgaggcgcgc
    42601 gatgacgtgc gccatcgtgt acgtcttgcc cgagcccgtg acgccgagca gcgtctggaa
    42661 cgagaggccg tcccggattc cttcgacgag cgcgtcgatc gcctccggct ggtcgccgcc
    42721 gggcggaaaa agctctgcta atttgaatgc cacgagtaga atgcgattgc gaaacccgcc
    42781 attttagacg aatgaacgcg ccacactcac cctccccgct cgcctcggtg ccgatggcgc
    42841 cggccgaccc gatcctcggc gtcaccgaag ccttcgtcgc cgacaccagc ccgaagaagg
    42901 tcaacctcgg cgtcggcgtg tacaccgacg accacggcaa gatcccggtc ttggagtgcg
    42961 tgcgccatgc cgagagcgag cggctgaagt ccgtgcccgc gcgcggctac ctgccgatcg
    43021 acggcctcgc ggcctacgac ctcgcggttc aggaattggt ttttgcttct caacaaagca
    43081 atattgtgac ggtgcaggcc ctcggcggca ccggcggcct gaaaatcggc gccgacttcc
    43141 tgaagcacat caatcccgcg gccgaggtct ggatcagcga gccgagttgg gagaaccacc
    43201 gccagctctt cgaggcggcc gggttcaagg tcaattccta ccgctactac gatccgacga
    43261 cgcgcggtct ggatgtcacc ggcatgctga actcgttgag cgccttgccg gccggctcga
    43321 tcgtggtgct gcacgcgtgc tgccacaacc ccaccggggt cgacctcgac aaggatcgct
    43381 ggggcgaagt gctggagatc gtgcgctcgc gcggcctcgt gccgttcctc gatctcgcct
    43441 accagggctt cgccgagggc ctcgatgctg acgcccacgc cgcgcggctg ttcgccgccg
    43501 ccatgtcgcc ggtcttcctg tcgagctcgt tctccaaatc gttctccctc tacggcgagc
    43561 gggtgggcgc gctgagcgtc gtcaccggca gccccgagga agcgaagcgc gtgctttcgc
    43621 aggtcaagcg catcgtgcgc accaactact ccaacccgcc cacccacggc tgccagatcg
    43681 ttgccacggt cctcggcaac ccccagctcc gcagcctgtg ggaccgcgag ctcggcgaga
    43741 tgcgcgatcg catcaaggcg atgcgaaagc ggctggtcga gagcatccat tcccgcgtgc
    43801 agaacaccga cttcagcttc gtgctgaagc agcgcggcat gttctcctac tccgggctga
    43861 cgaaggagca ggtgcggcac ctgcgcgagg agtactcgat ctacaccatc gacaccggcc
    43921 gcatctgcgt cgccgcgctc acctcgaaga acgtcgacta cgtctccgac gcgatcgcca
    43981 aagtaatccg gtaaaattcc gggttcttcc ggatccccga tagctcagtt ggtagagcga
    44041 cggactgtta atccgcaggt cgtaggttcg agccctactc ggggagccaa ctagctgttt
    44101 tcacgccgta ctagcgccag ctattttttc acgacctccc aagtcccttc gagcagcccg
    44161 tcgaggggaa tcagcttgta ggtgctttct ccgatcttct ggacgttaga gcacccgctg
    44221 ccaagattac tactgtactt ggtgcagaac ttgttgccct cgatccgcca ggagccaatg
    44281 tgcgtggtcg tggcggggcc ttcgttgaag cgcacagttc cgtccttctg atagacagcg
    44341 gtacccgtgc gggctgcggt gttcgggcgg tcggcccccg tgatcctgac gctcagcccg
    44401 ttcgacagca gcgccttcaa ctgcgcgttg gtaaggacag tcggctgcgg cttctgcgcc
    44461 caggcatggc cggcacagcc gagcatcgca actgcgagaa acgtcgtcag atttctattc
    44521 attgagtccc tccgtcgcaa atcctactcc gtgtgccgac gcgccgcatc tgattggacc
    44581 aagggcttat agtcggcgag ttcttctctg cggacaattc tgttttccga tggcaggagg
    44641 cctggcgcca tgcgcaagct cgatgttctg ctcctttgct gcaccctgac gttcgttgcc
    44701 ttctctttcc cggcggtggc ccaaaaggct gctgccgagc caaaaccgtt gccggaagat
    44761 ccactcggcc ggggcacgcc ctacggcgcg gtgatcgggt ttctgcaggc ggcgaacaag
    44821 gctgactatg agcttgcctc caagtatctg gagggcaaac agtcggccaa gagaaaagca
    44881 gaactcgcgc gcgatctcca tgtggtcttg aaccggggcg taaaaaccgg cccggacgat
    44941 ttgagcaaag cgccggcggg aaccctggac gacggtctcc ccgcgaacct ggaaaaagtg
    45001 ggcacggcga ccttcgaaag cgaaagcctg gacgtcgtgc ttcgcctcac gaagacaccc
    45061 gacacgcagc cgatctggct cttctccgct gagaccctgc ttggcgtgac tgcggcggct
    45121 agccaactcg atctcccttg ggccgaagcg atctggccgg aatcgttcag agacatccgc
    45181 ttcctgtctt acccactttt cacgtggctc aacgtgctcg tgggcgttcc gctcctggtg
    45241 gggattgcct ggcccctcac ccggggcctt ctcaggcttc ttcatccttt ggacctgcgc
    45301 cacggcgagg gtgtgctggc ccagatcaag tggctgctgt tccttctgat cttgtctctc
    45361 ctggcgcgta tcgtcgccac gcaagctgcc acagcggagg ggcgcatttt cgtcacctct
    45421 gttgccaacg tattcatcgt cgtggctgcc ggctggatct tggttcgggt gacgaaactt
    45481 gtggcccgat tgaagactca gcacctgcga aaggttgggt tgccgggcaa gatcgcgacc
    45541 gtcgaactag ccacctggct gtttgtatct gtctggttca ctgcggcttc gttcctgatc
    45601 ctgcgcagct tgggcttcga ccttacggcc gtcgttgccg gactcggcat tggcggactc
    45661 gcgtttgcgt tcgccgccca gaagacgatc gagaatctct tcggcacggt aacggtcgtt
    45721 gctgatgaag ccatccaggt cggtgactac tgccaggtag ggacaattga gggccgcgtc
    45781 gaaaacatcg gtctgcgctc cacccagatt cgcaccgccg atcgggtact ggtcagcgtt
    45841 ccgaatgggc agcttgccgc ggggactatt ggaaatctgg cccggcgcga caaatttctt
    45901 ttccgccata acattctgtt ggcatacgaa accacggccg accagcttcg ccatgtcctc
    45961 gcggagatca ggaaaatgat gctcgcacaa gccaagctcg aacctgaaac cgtcacgacc
    46021 cgattgatcc ggtttggcgg cgcctccctc gagctggagg cctttgcgta tgtggcaact
    46081 cgcgatgagg gtgtcttcct cgatactcag gaagaacttc tactgcggat catggatatc
    46141 gtcgaagcca gtggagtgcg gcgcgtagcg gcgtagaatc gcgaccgcaa atgagcgccg
    46201 cgctgcaact cctgctggtc gcgctctgcc ttgtgctcgc aggcacggcg cgcgcggacg
    46261 actggtcggc gtggcaacgc gcctacgatc cggcaacgcg cacacgtttc attcccgtag
    46321 agctctggac cggcgcgcct tgggacggca cgcaggagat ccgcatggcg cccgctgcgc
    46381 tcgagttcgg gcagcgggga gacaaaagca tcaaggggcc gacgacttgg aacttcatcc
    46441 aggtctacga gcgcgtgaac cgcgacaagc tccaacgctt cgccatccgc gacgacaaga
    46501 cgggcctggg ccgcgttttc gactcgcgct atccggagct tgactgccgc ggcgaggtga
    46561 aatttccgct cggccgctgg aagcagggtg aggtacgcga gtaccagctc gactgcgcgg
    46621 gcgacaggcg gccgcgcccg cgtccgctca aagtgacgat cgaggagatt gatttcgtct
    46681 acggcggcgt accgcactcg ctgcgcttcc attggctctt catggaaggg cgcggccgcg
    46741 gcaccgacat gcgctacgtg tacagcccgc tgcgcggact ggtcaacgtg caggggaacg
    46801 agtagagcct cacgacacca cgagcatcag gacgatcgaa agcacaccga cgatcagcga
    46861 gcacaaaccc gcccccagca gcactctgtc gccgaaggtc agttctttca tttccatttg
    46921 agtcccctga gttaggaaat aatttcgacg aaggcatgat gccgagtgtc ggcttgagga
    46981 cgttatcccc cgcgcgccag gcgttttccc ccaggcgcca atcgcactag cggctggctg
    47041 gggctatgcg atctgcgctc tcgctccggt agcaagacgt gcagtattct tcccgtcaag
    47101 caaagcaggg agaaccacag tgagtgagtc aggcggcgga cgcgccggcg atcagtcgcg
    47161 ccagagtttc cttgcctcga tgggccagat gttccgacgg ctccttgaat tgcacggcct
    47221 cgacgcagtc ggcatcgctc ggcatgcggg tgtcgacctc gcggccatcc ccgctccggg
    47281 cgagcgcatc gaagtcgaca agatcgacgc gatactccgc gtcgcgatcc cgctcgtcag
    47341 tgatcccgcc ttcggggtcc aagccgcacg ctgctggcac ccctcggaac tgggcgtcct
    47401 tggatatgcc tggctgtcga gctcgacgct tcgcaccggg ctcgaacggg ccgcgcgcta
    47461 ttcgcgcctg gtaggcgagc gcggcattac cgagatcgag gacaccaggc aggggctcaa
    47521 agtccgcttc tgggccaacc gcggcaatcc cgccgtcgtg ccggtggctg cggtcttcgt
    47581 cgacatcgcg atggcgatcc tgctcgatct atgccgcatg aacgcaggag cggctctgcg
    47641 gccggttggc gcgtccctgc gtcgccgcaa gcccgaaccc gcagacgcct acgcgcgctt
    47701 cttcggatgc ccggtccagt tcggcgccga ggagaacacg ttcgtgcttt cggccaaaga
    47761 cgctgactgc ccgctcccgt cggcaaaccg acaactcgca gcagtgttcg acaagatgct
    47821 tatcgaggag ctggcccggc tagacaagtc cgacgtcgtc tcgcggtgcc gtgcggcggt
    47881 gctgcaggac ctgtcctcgg gcgaaggaac tgcggaagat acggcgaagc aacttcacat
    47941 gagcccgcgc acgctacagc gcaagctcgc cgaggcgaac acgacttacc ttcagctcgt
    48001 cgacgacacg cgcaaagacc tggccttacg ctacatcgaa gacccgcgcc gctcggtcac
    48061 cgacatcacc ttctcgcttg ggttctccca gccgagcgcg ttcactcgcg cgttcaagcg
    48121 ctggacgggg ctcagtccaa gcgactaccg gacgaagtcg cccaccttcc gctagcagca
    48181 agcaggcccc gacgatggcg cgccagggtt agcgcctggc gtcagcggtc aagttccttt
    48241 cttgggtctc ggcgatagtt cgccaccacc cgcgctgcga acacgcagtc gcggcacacg
    48301 acttgaggca acccgaaaat ggactaccgg ctgcagaaaa tggattaccg gctgtttcgt
    48361 ctccgcgatg gcgatcccat cgaagctttg gaagcgcacg tcctggcccg cgcctacgtc
    48421 gacgcttggc gtgcgctgta tctatgcgaa ccgatcggcg agcacgccat cgatagcctg
    48481 ggtctcgcga tcgattttgg ccgtcgtgct cgccgtcttc aatagtcgct ctcccatgaa
    48541 gaagctgcac cttgaacagg ccgcgcagtg cgggacgctt gatcaagccg aaagcgtatt
    48601 gcgcctgagc gcgccgcggg cgtggtgggc cgtcgagcgc acggagacgg ccgtggtggt
    48661 ctcgcacatt tgggaaaacc gtgagccgat tctgtgcgcg ctgattgtgg agcgcgcacc
    48721 gtgcggcgcg caagaacact cactggaggt gccgtaatgt tggccgtatt gttctggacc
    48781 ctgttctcgc tggtgctgct gatgatcgtc cctgatcgct ggcatgcccg gcgcgaaatc
    48841 gccaaacgcc aagtgggagt acgccgacag tccccaagga cgcgttcgcg cgcctagtct
    48901 cgggcggacc cggcctgggc caaagcgttg tgtaccgtaa agggaaggag aggttgcagc
    48961 gcctcgacgg ccgggtccaa cctgcaccct acgccggtcg accagtgccg tatgttcgat
    49021 gccgtacaca accgccagaa tcaccagcgc ggcgacgccg ctcgtcgttc gatcgttacg
    49081 gccagacgaa gtcgcgtacg acgtctctta ttcccgacgc ctcccccgga gcaaatcact
    49141 tttccttcac ttggcgccgc ttccagcgcc ggccaggtcg gggctgcgac ggatccggcg
    49201 ccattcccac ggcgccatcg gatttgtcgc cgaccacaag ccgcggcgag atagtcgcgc
    49261 agcatcctgc tgggcataca ggctgtagtc ggtcacgtag cgatcgaaca cccacgccat
    49321 gccgcgacgg acctgctcga agttggcgtc tctgccggca cacgtcacgc ggccgatggt
    49381 gcggccgttt cggtcttttc catgctcacg gatgaacgca cgttgtgaca tgcacagctc
    49441 cgccagcgaa tccttggagc gctggccgaa cgcttggcgc atttcgggcg catcgatgct
    49501 gtccagccgg acgcgcgttt gctttgaacc tgctagaacc gtaagggtgt cgccgtcctg
    49561 cacggcgacc acatggccgg agaagtctgc gagggccagc cacggtatcg ctgcgaaggc
    49621 aagcagcgca aacctaacgg cgtcgccgag gcgcacgagg ctgaacttcg cggattgcgc
    49681 ggaccgactt gaggagtcgc accagcgccc gcgcgccctc gggcgtcgag tcccaggtgc
    49741 tcagcgccgc gcgcacgacc gcgtccttgg cggtcggcgc cggctcagcg acgcccgcgg
    49801 cctgcgcctt tggatcggcg gcgtaggcga gcagcttgcg cacgttcgag gacacccgct
    49861 tgaactggcc gcggaagagg cgcgacacct ggctctggtg gatccgggtg tcggcggcga
    49921 cgcgggtggt ttgcacgcgc tcggcgagga ccgccagcgc gcgccgagcc tcagccagct
    49981 cgcgatcggg atcggggcgt ttgacattca tttgcatttt gcatatcgtc ctgcatatgc
    50041 ataataagac cggacggccg gcggtggcaa cttgggcggc agcgagctag aaaaactcgc
    50101 cccgccggcc gcaacccggc acaccacggt gctgtgcgcc gaggtcggcg cgccgccgca
    50161 gcccgccgcg cgctacctgg aggtgctcgg gcaggcggct gagctgagcg gcggtcgcgt
    50221 gctgcacacg caccccaacg gcgtgctcgc gctcttttcg acgccggatg cggccgccgc
    50281 cgccgcggcg cgaatgcacg tgtacgcgca ggcgctgccg ccggagcagc cgaagctcga
    50341 tgtgcgcatc gggttccatg ccggtccggt cgcacagcgc aacgaggaca tcttcggcga
    50401 cacggtcaat ctcgcgctgc agcttgccga tcacgcaagg ggcggccaga tcctcacctc
    50461 ccacgacacc gcctccagtc tcacgccggc cgtccaggga ctggtgcgcc ccatcaagca
    50521 tctgcggatg caaggaaaga agagcgacct gctgctcggc gagctgatgt ggaaggatgc
    50581 ggtgaaccag atcgtgtcgg cccgccgcgc ctcttccccc gcgcgcgcgg ttctgcgcgt
    50641 cacctaccgg ggcaaggcga tcttccgccg ccgggagggc gacacgatgg cgctaggccg
    50701 ggacccgcaa tgcgacctga aggtggatga cgccgccgtt tcgaggcagc actgcacgat
    50761 cgcgcgtaag ggcgcggtct tcgtccttcg cgaccacagc agcaacggca cgttcgtcac
    50821 cgtcgcgggc cagagcgagg cacgcattca cgaccaggag ctcgcgctcg gcaaatcggg
    50881 cctgatcgcg ttgggccagt ccgcacccac gaccgagcac gtcatcagct acgtctgcga
    50941 accccaaaac taaggggccg ctgcctaagg tttgcgcgga aagtgcgcca gaaagtgcgc
    51001 gacgtcgatc aaatcctgat cgttcaagcc tgaaacagtc tcgggcatga ccgccatgcc
    51061 gtagccggtg cggaccccct tcttatagtc gcgcaatgcc ttgaggaggt agtcctcgcg
    51121 ctggttggcg aggcgcggca tgttctcgcg gccggagtag tcggggttat ggcagttggc
    51181 gcagcgctcc ttttccgcga tcgcgcggcc acgcgcgacg cgtgcgatct cgggcttctc
    51241 tgccggacgc gggggcggcg gcatcttggc gatcaggtcg gcgaatgcct gcagctcggc
    51301 gtcggtcagg cccgcagcgg cggcgttcat gaggacgttg tcgcgccggc cttcacggaa
    51361 caggaaaagc tgcgcgagga cgaagaaagc gggctggccg ccgagcgagg gcgtgagcgg
    51421 cgtcgacgac gtgcccttcg cgccgtggca ggcgaagcag gcctccttcc gctgcgcgac
    51481 cggctgggcg gcagcggaaa gagccgttgc tagcagggca attccgaccc cgcgggtcac
    51541 ttcccgcgca ttactgcccg ccgcattact ttccggtata cgtgatgcga tagatcgcgc
    51601 cgttgtggtc gtcggaaacg agcagcgacc cgtccttcat caccatcacg tcggccggcc
    51661 ggccgaggta ggtgttcttt tccaccagtc cggtgaggaa cggctccatc ctggcgttgc
    51721 ccttggcgtc gatgaagacc gcgacgacgt cggccgcgta cttctgcgtc cggttccaag
    51781 ggccgtggcg cgcgaggaaa atcgcgttct ggtacttggc ggggaacatc ttgccggtgt
    51841 agaagcgcat gccgagcggc gccgagtgcg cgccgatgag cagcgccggc ttggcgtaat
    51901 cgctgcactt cttgccccag ccgaactgcg gatcggtcat cacccccgaa tggcagtacg
    51961 ggtatccgaa atgctgcttg ccgggctggg tgacgcggtt cagctcgtcg ggcggcatgt
    52021 cctcggacag ccagtcgcgc tggttgtcgc cgaaccacag ctccttggtg gtggggtgaa
    52081 aatcgaagcc caccgtgttg cgcatgccct ccaccaccga ctcgaagccg gtgccgtcca
    52141 ggttcatgcg gaagatgcgc gagtacgccg ccgaaggctc gcagatgttg cacggcgcgc
    52201 cgacggggac gtagagcttg ccgtcgggcc cgatcttgat gaacttccag ccgtgcggca
    52261 cgtcgcccgg cagcttgtcg tacaccatcg ccgccttggg cggattgtcg agcttcgctt
    52321 cgatgtcgtc atagcgcgtg atgtccttgg gcgtggcgac gtacagcgag cccttgtgaa
    52381 actcgatgcc gttcggcagg aacagcttct cggcgatggt cttcacttca cgcttgccgc
    52441 cgcggtcgac caccgcgtag atcttgccgg cgacgaaaag cgagctcacg aacaccgtcc
    52501 cctggtcgcc ctcgcgcatt ccgcgggcgt cgaggatgcc gctcacccac acctcggcct
    52561 tgaagccggg cggcagcttg atcttcttga gcggaagctt gtcggcggcg gtcggaaccg
    52621 ggtgcgccgg aaccggcgcc atcttgtcgg cggcatctgc tttgggcctg ccgatcgccc
    52681 agaagggctc ctccttcttc tcctgcgcca gagcctggga tgccgccagt atcgcggtga
    52741 ccgcgattgc gaaaactctc atgccccacc tcccttgatg aacgtcgaag agcttacagc
    52801 gcaaggccga tctcggtcga gggtgagcca tacgccttct ggaacaccac gcggtagctc
    52861 tcgagcgcgc cgtcgcgccg gaactggtgc gcgcagcccc aaagatagag ctggaacagg
    52921 cgacaagtgc gctcgccata gcgccgcgcg agctcgtcgt gcgccgcctc gaggttgcgc
    52981 gcccattgct gcagggtcag gaaatagctg tggcggtcgt tgtgcacggc gatcgtctcg
    53041 aacgccgtgc cgttggccgc cgcgagcagg tcgggcatca cgaccggcgt gtgcgagccg
    53101 ggaaacacgt agcgataggt gaacgagctg acgttgaact tccggcggtt ggacgcgaaa
    53161 tccatgtaca gccgcccgcc ggtcttgagc agccggtcga actgcgcgaa caggcgcttg
    53221 tagttgggca gatgctccat cacgccgagc aggacgatgg cgtcgtactg ccggctcgag
    53281 gcgtaggcga agatgctctc atagaccacg tccaccctgc ccttgcggag gaagtcgtac
    53341 tgctcgcgcg agatcgtgag cgcggtgacc ttcgctccgc gcgccccggc gtaacccgcg
    53401 aacgagcccc atccgccgcc gacgtcgagc acgctcgacc ccggcgatag gcggcacgcg
    53461 cgcatcacgt aatcgagctt gttctccgcg gctttctcca gcggttcgtc gtcggcgtga
    53521 tagagcgctt gcgaatagag cctgaaacgc cggtcgagga aagcgaaata gaaatcgttg
    53581 ccgtagtcgt agtgccgcgc cacccagtcc ttgtcggtgc gcacggcacc gaagaggacc
    53641 ggccgcatga accgccagag cgagtgcagc gggtgccagt cggacgcgat cctgcgcagg
    53701 tccagggcag ccagaaagct gcccgcgaaa tttatctctt cgtctatgta ggcttcgcag
    53761 atcgcagttt cgtcgaggga ttccagcgcg cgagccgctc tcgcggtgcg caggtgcagg
    53821 ctgaaggcgg gagcgccggc gccgatccgg tgcaccgcgc cgtcgggcaa ctcgacatcg
    53881 aatggcgcca tcgcgacgcc gcccaggcgg cgcctgagct tcaggatgac ggacggctcg
    53941 tcggcggatt ccgccgtggt cgaaggcagc atttatcgtt ctgaactgcg gcgagaatgt
    54001 agcattgccg cagatgaagt gctttcggtg caatagcgaa acaccggccg acgcgaagtt
    54061 ctgtccggcc tgcggcgccg gtctgaccgc cgcgccctcc ccccagagct acacgcccaa
    54121 gcacctcgcc gagaagatcc tgcgctcgcg ccacgcggtc gtcggtgagc gcaagcagat
    54181 cagcgtgctc ttcgccgaca tgaagggctc gatggagctc ctcgccgagc gcgaccccga
    54241 ggaagcgcac aacatcctcg acccgatgct ccggcacatg atggacgcgg tccatcgcta
    54301 cgaaggcacg gtgaaccagg tgctgggcga cggcatcatg gcgctcttcg gcgcgccgct
    54361 cgcccacgag gaccacgcgg tgcgcgcctg ccacgccgcg ctccgcatgc agcagacggt
    54421 cggctggtac gcggaggagc tgcgccgcgg ccgcggcgtg gacgtgcaga tccgcgtcgg
    54481 gctcaactcg ggcgaggtgg tggtgcgcgc gatcgacagc gacctgcaca tggactactc
    54541 ggccatcggc cagaccacgc acctggccgc gcgcatggag cagctcgcgc gccccggatc
    54601 caccctgatc tcgcgcgaga ccctgcggct ggccgagggc tatgtggaga tcaaggcgct
    54661 cgggccggtg ccggtgaagg gcatgccgga gccggtcgag atcttcgagc tggtccgctc
    54721 cgagcaggtg cactcccgtc tcgaggccag cgccgcgcgc ggcctgacgc ccttcgtggg
    54781 ccgcgaagcc gaggtggacg cgctccacca ggcgatggcg cgcgctgcgg cgggccacgg
    54841 ccaggtggtg gcggtgtcgg gcgagccggg cgtcggcaag tcgcgcctgt tctgggagtt
    54901 cacccactcc catcgcgcgc acggctggct catgatggag gcgcaggcga cgtcgtacgg
    54961 caagaacacc tcgtacctga cggtcaagga gctgctccag aactatttcc acctggagga
    55021 gcgcgatgac gtccggcgcg tgcgcgagaa agtcaccggc aagctgctga cgctggacga
    55081 gagcctgcgg ccgaccctgc ccgcgttcct ccagctcctc gacgtgccgg tggacgacgc
    55141 ggagtggcag accccggatc tgccgcagcg ccgccagcgc atgctcgacg cggtcaagcg
    55201 gctcattctc cgggaaggcc aggtgcagcc cgtgctcctg gtgttcgaga acctccactg
    55261 gctggactcc gagagtcagg ccttcctcga cagcctggtg gacagcctgc ccctggcccg
    55321 cgccctcgtg ctcgtgagct accggcccga gtaccagcac gggtgggcgc ggaagagcta
    55381 ctacacccag ttccggatcg atccgctccc gccccagagc tcggaggagc tgctccgcgc
    55441 gctgatgggc gacgaccccg ggctgggccc gctcaag
//
LOCUS       DATBSZ010000232         5248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_520139, whole genome shotgun sequence.
ACCESSION   DATBSZ010000232 DATBSZ010000000
VERSION     DATBSZ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520139"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VIG70_14890"
     CDS             complement(<1..1206)
                     /locus_tag="VIG70_14890"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8251884.1"
                     /translation="MASPFAVRSFRFQWPADLAASWAFEMEALILGWYILSTTGSVQQ
                     LVVVAALVWLGSLFSPFFGLAGDRIGHRTMLCITRGVYALLAAVLTALILTEALATWH
                     VFAIAGLAGLVRPSDMVMRNTLVGQTMHPDVLMRALAISRTTSDTARVAGAVTGTAGV
                     AWIGMGPAYAVVTAMYVASFLLSLGVARTPRAVAAAHPLSDLREGFRHVWEKPDLLGA
                     FSLAFLVNLLAYPFVLGLLPYVAKEIYAVDQSGLGYLAGAFWVGALAGSLLVSASVFP
                     MRAARVMLWSGALWFAAVLLFGQTTSLPFGMGFLFVAGAAQSFCLTPLAAVMLRSSDE
                     AMRGRVMGVRILAVWGLPLGLLAAGPIIALIGFAATTLLYSAIGLAATFAIGYGWRHS
                     LWHRAAAANA"
     gene            1225..1635
                     /gene="tadA"
                     /locus_tag="VIG70_14895"
     CDS             1225..1635
                     /gene="tadA"
                     /locus_tag="VIG70_14895"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY8251885.1"
                     /translation="MKDEEYMHRALELARRAQDEGEVPIGAVVVLDGEIIGEGWNRPI
                     AASDPTAHAEIQAIREAAKKLGNYRLTSATLYVTLEPCDMCIGAMFHARVARAVYGAS
                     DPKKLVLKNEVVLQGGVLAQECGALLSKFFAAKR"
     gene            1632..2090
                     /locus_tag="VIG70_14900"
     CDS             1632..2090
                     /locus_tag="VIG70_14900"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY8251886.1"
                     /translation="MNMVIDIGKQELRYGDKTYAVSTSKFGPGEKNGSFCTPRGSHIV
                     RAKIGAGQPLGAVFVRRRPTGEVWSPDLHERYPGRDWILTRILWLSGCEPGRNRLGEV
                     DTMRRYIYIHGSPDSAELGKPGSIGCVRMRNRDVAELFDLVPAYTPVSIR"
     gene            2166..3257
                     /locus_tag="VIG70_14905"
     CDS             2166..3257
                     /locus_tag="VIG70_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251887.1"
                     /translation="MTRVLASLLLVSMLAGTSAALAQSCGIYPRTITTLKEKGGRVDW
                     SRAHGLIAYDDDRNADGYFDIRVMSRDGSVDYCLTCWEPQQAPPGLKKNKGQPAWHPS
                     GNHLVFQAEFDDSSVGPKASNPGRGVNNVLWLTDRNGTEFHPLTLPSTVTPATGVLHP
                     HFSDDGEKLSWSEMYEPSAIGQAGKAAGFWRLMTAKFVAGNPPRLENIRAHLPMAEGF
                     YENHGFSPDGSLVIFSSNAARKGYLDRLNNDIFTLDLQSGAVTRLTDTRYNEHASYFP
                     GGRKILWMSNRENTSRGTDLWIMNPDGSQKERLTFLNRSGCPEETPNRAVAADSSINA
                     TGDAFVVYVQDELLGDVGSILLVELAFPF"
     gene            3319..4566
                     /locus_tag="VIG70_14910"
     CDS             3319..4566
                     /locus_tag="VIG70_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY8251888.1"
                     /translation="MKHRTLTRALALAAALCAIPVSPALAHGDGDRHDDEARRPLVIG
                     HRGAHGYLPAHTLEGYALAIELGADYIEPDLVVTKDGHLIASHEPNLIATTDVASRPE
                     FASRRRTGVVDGASEVGFFASDFTLAEIKRLRRVQDFAERPQQFNGKFEIPTLEEVIA
                     LAKRKSEEKGRTIGVYPETKHPTYHKSLGLPMEKKLVAALAAAGWNRRDAPVFIQSFE
                     QSNLKELNRLTPVRLVQLVDANDVNPDGSLDYTAPFDRPYDWTVSGDPRLLARTFGFF
                     ATDAGLREIKTYADGIGPWKRYIVSSVPIEGSTAPGEASRKAVPPPVNPDLIRRAHKV
                     GLLVHTWTFRNEQRRLLSDYAGNPVNEYLEFYRLGIDGVFADFPDTAVVSRVLFDLER
                     DRDAGRCLTGERDGPKRHQPDCD"
     gene            complement(4517..>5248)
                     /gene="mtnA"
                     /locus_tag="VIG70_14915"
     CDS             complement(4517..>5248)
                     /gene="mtnA"
                     /locus_tag="VIG70_14915"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEY8251889.1"
                     /translation="MRKAKDLKAEAEAIFTEDIAANRAMGKLGAALIPRKARVMTHCN
                     TGALATAGHGTALGVIRSSKNKSISVVACETRPYLQGARLTAYELMQEGIPCTLITDS
                     MAGHLMSRGEVDVVVVGADRIAANGDVANKIGTYPLAVLAKRHGIPFYVAAPLSTFDP
                     KIANGSRIPIEERAADEVTGYRGSRWAPEGVAVRNPAFDVTPAELITGIITERGIVRK
                     PTRARVKALLSRSRAGAASGRRARR"
BASE COUNT          950 a         1791 c         1745 g          762 t
ORIGIN      
        1 agcattggct gcggccgcgc gatgccacag cgaatggcgc cagccgtagc cgatggcgaa
       61 ggtcgccgcc agcccgatcg cggaatagag gagcgtggtc gccgcgaagc cgatcagcgc
      121 gatgatcggc ccggccgcga gcaggccgag cggcaggccc cagaccgcga ggatgcgcac
      181 gcccatcacg cggccccgca tcgcttcgtc cgacgagcgc agcatgaccg cggccagggg
      241 cgtgaggcag aagctctgcg ccgcccctgc cacgaagaga aatcccatgc cgaagggcag
      301 ggacgtggtc tgcccgaaca gcaggaccgc ggcaaaccac aaagcgccgc tccagagcat
      361 gacgcgcgcg gcgcgcatcg gaaaaacgct ggcgctgacc aggagcgagc ccgccagcgc
      421 tcccacccag aaagccccgg caaggtagcc cagtccggac tggtcgactg cgtagatctc
      481 cttggcaacg tagggcagca gcccgaggac gaaggggtag gcgagaaggt tgaccagaaa
      541 tgccaggctg aaggcgccga gcaggtcggg cttctcccac acgtgcctga atccctcgcg
      601 caggtcggag agcggatgcg ccgccgcgac cgcgcgcggc gtgcgcgcca ccccgagcga
      661 cagcaggaac gaggcgacgt acatcgcggt gacgacggca tacgccggtc ccatgccgat
      721 ccacgccacg cccgcggtgc cggtcaccgc gcccgccacc cgggccgtgt ccgaggtggt
      781 tcgcgaaatg gcgagggcgc gcatcagcac gtccggatgc atcgtctgtc ccaccagggt
      841 gttccgcatc accatgtcgg agggccgcac gagcccggcc agccccgcga tcgcgaacac
      901 gtgccaggtc gccagcgcct cggtgagaat gagcgcggtc agcacggccg cgagcagcgc
      961 gtaaacgccg cgcgtgatgc agagcatggt tcgatgcccg atgcggtctc ccgccaagcc
     1021 gaagaacggc gagaagagag agccgagcca gaccagcgcg gcgacgacca cgagctgctg
     1081 caccgacccc gtcgtggaga ggatgtacca gccgaggatc agggcctcca tctcgaaggc
     1141 ccacgacgcg gcgaggtccg cgggccactg gaaacggaag cttcggaccg cgaagggcga
     1201 tgccatctgc gaaaatcagg ggtaatgaaa gacgaagagt acatgcaccg cgcgctggag
     1261 cttgcacggc gcgcccagga cgagggcgaa gtgccgatcg gcgcggtggt ggtgctggat
     1321 ggcgaaatca tcggcgaagg ctggaaccgg ccgatcgccg cgtccgaccc aaccgcccat
     1381 gccgagatcc aagcaatacg tgaagcagca aaaaagctcg gcaactaccg cctcactagc
     1441 gccacgctct acgtgacgct cgagccctgc gacatgtgca tcggcgccat gtttcatgcg
     1501 cgggtcgcgc gcgccgtgta cggcgcttcg gatccgaaga agctggtgct gaaaaacgaa
     1561 gtcgtccttc agggaggcgt tctggcgcag gagtgcggcg cgctcctttc gaaattcttc
     1621 gcggccaagc gatgaacatg gtcatcgaca tcggcaagca ggagctgagg tacggcgaca
     1681 agacctacgc cgtctccacc tccaagttcg gcccgggcga gaagaacggc agcttctgca
     1741 cgccgcgcgg cagccacatc gtgcgcgcca agatcggcgc cgggcagccc ttgggagcgg
     1801 tgttcgtgcg gcgccgcccg acgggcgaag tgtggtctcc cgatctgcac gagcgctatc
     1861 cgggacgcga ctggatcctc acccgcatcc tgtggctgtc gggctgcgag ccgggccgca
     1921 atcgcctcgg cgaggtcgac accatgcggc gctacatcta catccacggc agcccggaca
     1981 gcgccgagct cggaaagccc ggctcgatcg gctgcgtgcg catgcgcaac cgcgatgtgg
     2041 ccgagctgtt cgatctggtg ccggcataca cgcccgtttc cattcggtaa agctgtattg
     2101 ccgggaggta aagtcgcgcg cgtcacggac gaaacactcc taagatcgat tcacgggagg
     2161 tttcgatgac acgcgttctc gcctcgctgc tcctcgtgtc gatgctggcc ggcaccagcg
     2221 ccgcgctcgc gcaatcctgc ggcatctacc cacgaaccat caccacgctc aaggagaagg
     2281 gcgggcgcgt cgactggtcg cgcgcccacg gcctgatcgc ctacgacgac gaccgcaatg
     2341 ccgacggcta cttcgacatc cgcgtcatgt cgcgcgacgg ctcggtggac tattgcctga
     2401 cctgctggga gccgcagcag gcgccgcccg ggctgaagaa gaacaagggg cagccggcat
     2461 ggcatccgtc cggcaaccac ctcgtgttcc aggcggaatt cgacgacagc agcgttgggc
     2521 cgaaagcctc gaatcccggc cgcggcgtca acaacgtgct ctggctcacc gaccggaacg
     2581 ggacggagtt tcacccgctc acgctgccct ccaccgtcac gccggcaacc ggcgtgctgc
     2641 atccgcactt ctccgacgac ggcgagaagc tttcgtggtc cgagatgtac gagccttccg
     2701 ccatcggaca agcgggaaag gccgcgggct tctggaggct gatgacggcg aaattcgtcg
     2761 cgggcaaccc gccgcgcctg gagaacatca gggcgcacct ccccatggcc gagggcttct
     2821 acgagaacca cgggttctcc ccggacggct cgctcgtcat tttctcgagc aacgccgccc
     2881 gcaagggata cctcgaccgg ctgaacaacg acattttcac cctcgacctc cagagcggcg
     2941 ccgtcacgcg cctcaccgat acgaggtaca acgagcacgc gagctacttt cccggcgggc
     3001 gcaagatcct ctggatgtcc aaccgggaaa ataccagccg cggcacggac ttgtggatca
     3061 tgaacccgga cggcagccag aaggaaaggc tgactttcct gaaccgcagc ggctgtccgg
     3121 aagagacgcc gaaccgcgcc gtggcggccg acagcagcat caacgcgacc ggcgatgcgt
     3181 tcgtcgtcta cgtccaggac gagctcctgg gcgacgtcgg ctcgatcctg ctggtggagc
     3241 tcgctttccc gttctagcaa ccaaaaggta attgcagcgg agtagagttc tcccgttgtc
     3301 acccaacggg aggaaggaat gaaacaccgc actcttacgc gcgcgcttgc gctcgctgca
     3361 gccctttgcg ccatccctgt ttcgccggcc ctggcccacg gcgacggcga tcgccacgac
     3421 gacgaggccc gccggccgct ggtgatcggc caccgcggcg cgcacggcta cctgccggcg
     3481 cacacgctcg aaggctacgc gctcgcgatc gagctcggcg ccgactacat cgagccggat
     3541 ctcgtcgtga ccaaggacgg gcacctgatt gcgagccacg agccgaacct catcgccacg
     3601 actgatgtcg cgagccgtcc cgagttcgcc tcccgccggc gcaccggcgt ggtcgacggc
     3661 gcctccgagg tcggcttctt cgcttccgac ttcaccctcg ccgagatcaa gcgcctgcgc
     3721 cgggtgcagg acttcgcgga gcggccgcag cagttcaacg gaaaattcga aatcccgacg
     3781 ctggaagagg tgatcgcgct cgccaagcgc aagtcggaag agaagggtcg caccatcggc
     3841 gtctacccgg agaccaagca cccgacctac cacaagtcgc taggcctgcc gatggagaag
     3901 aagctggtcg cggcgctggc ggcggccggc tggaaccggc gcgatgcgcc ggtgttcatc
     3961 cagtccttcg agcagtcgaa cctgaaggag ctgaaccgcc tgacgccggt gcgcctggtg
     4021 cagctggtcg acgcgaacga cgtcaacccc gacggcagcc tcgactacac cgcgcccttc
     4081 gaccgtcctt acgactggac cgtgtcgggc gatccgcgcc tgctcgcgcg caccttcggc
     4141 ttcttcgcca ccgacgccgg gctgcgcgag atcaagacct acgccgacgg catcggcccg
     4201 tggaagcgct acatcgtgag ctcggtcccg atcgagggct cgactgctcc cggcgaggcg
     4261 agccgcaagg ctgtgccgcc gccggtcaat ccggatctca tcaggcgcgc ccacaaggtg
     4321 gggctgctgg tgcacacctg gacgttccgc aacgagcagc gccgcctgct ttcggactat
     4381 gccggcaacc cggtcaacga gtacctggag ttctaccggc tcgggatcga cggcgtgttc
     4441 gcggacttcc cggacaccgc ggtggtgtcg cgcgtgctct tcgacctgga gcgcgaccgg
     4501 gacgccggcc gctgccttac cggcgagcgc gacggcccga agcggcacca gcccgactgc
     4561 gactgagcag ggccttgacc cgggcgcggg tcggctttcg cacgatcccg cgctcggtga
     4621 tgatcccggt gatgagctcg gccggggtga cgtcgaaggc ggggttgcgc accgccacgc
     4681 cctccggcgc ccagcgagag ccgcggtagc cggtaacctc gtcggcggcg cgctcctcga
     4741 tggggatgcg cgagccgttc gcaatcttcg gatcgaacgt ggagaggggc gcggcaacgt
     4801 agaacgggat gccgtgcctc ttcgccagca cggcgagcgg gtaggtgccg atcttgttgg
     4861 cgacatcgcc gttggcggcg atgcggtcgg cgcccaccac cacgacgtcg acctctccgc
     4921 ggctcatcag gtgcccggcc atgctgtcgg tgatgagcgt gcaggggatg ccctcctgca
     4981 tgagctcgta ggccgtgagg cgcgctccct gcaggtaggg ccgggtttcg cacgcgacca
     5041 cggaaatgga tttgtttttc gaagaccgga tgacccccag ggcggtgccg tggcccgctg
     5101 tggcgagcgc gccggtgttg cagtgggtca tcacgcgcgc cttcctcggg atcagcgcgg
     5161 cgccgagctt gcccatggcg cggttggcgg caatgtcctc cgtgaagatc gcctcggcct
     5221 cggctttgag gtccttggcc ttgcgcat
//
LOCUS       DATBSZ010000233         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_43308, whole genome shotgun sequence.
ACCESSION   DATBSZ010000233 DATBSZ010000000
VERSION     DATBSZ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43308"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VIG70_14920"
     CDS             <1..774
                     /locus_tag="VIG70_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8251890.1"
                     /translation="PVKGAAMTAAVIALKPTFKPMNNHKFEVSCASCNLREICLPAGL
                     CLDDLERVENMVYARRRIKRGEALFNAGAQFNAVYAIRSGFLKTSVLDSEGREQVTGF
                     FMGGELVGLDGLGSGTYNGTAVALEDCEICVLPYARIEEMAREVPALQRHLHAVLARE
                     IVRDHGVMLLLGSMRAEERLATFLLNLSRRFVRRGYSASDFHLRMTREELGSYLGLKL
                     ETVSRLFSQFQADGLIDVQQKHVRILDIAGLEQVLAAKH"
     gene            797..1240
                     /locus_tag="VIG70_14925"
     CDS             797..1240
                     /locus_tag="VIG70_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8251891.1"
                     /translation="MFKHILIPTDGSPVSAKAVRAGIAFAKQSGAKVTGYHAVEPVPT
                     HLYGEGYIADKAMVAEFERRRRDAAKKRITAVAREARKAGVPFEPLVQIARTPYEGIV
                     DAARKRKCDLILMSSHGHRGFSRLVLGSVADKVIQLSKVPVLVYR"
BASE COUNT          262 a          522 c          491 g          226 t
ORIGIN      
        1 cccgtgaagg gagccgccat gaccgccgcc gtgatcgccc tcaagccgac cttcaagccc
       61 atgaacaacc acaagttcga agtgagttgc gcgagctgca acctgcgcga gatctgcctg
      121 ccggcggggc tgtgcctcga cgacctggag cgagtcgaga acatggtcta cgcacgccgc
      181 cgcatcaagc gcggcgaagc cctgttcaac gccggcgccc agttcaacgc ggtctacgcg
      241 atccgcagcg gcttcttgaa gaccagcgtg ctcgacagcg agggccgcga gcaggtcacc
      301 gggttcttca tgggcggcga gctcgtcggc ctggacggcc tgggctccgg cacctacaac
      361 ggcaccgcgg tcgcgctcga ggactgcgaa atctgcgtgc tgccctacgc gcggatcgag
      421 gagatggcgc gcgaagtgcc cgcgctgcag cgccacctgc atgcggtgct cgcgcgcgag
      481 atcgtgcgcg accacggcgt gatgctgctg ctcggctcga tgcgcgccga ggagcgcctc
      541 gccacgttcc tgctcaatct gtccaggcgc ttcgtgcgcc gcggctactc cgcctccgac
      601 ttccacctgc gcatgacgcg cgaggagctc ggcagctacc tcggcctcaa gctcgagacc
      661 gtgagccgcc tgttctcgca gttccaggcc gacggcctga tcgacgtgca gcagaagcac
      721 gtgcgcatcc tcgacatcgc cggcctcgag caagtgctcg cggccaagca ctgagaaccc
      781 tacaaggagc acgaccatgt tcaagcacat cctgattccc accgacggct cgccggtctc
      841 ggccaaggcg gtcagagccg gcatcgcgtt cgcgaagcag tcgggcgcca aggtgaccgg
      901 ctatcacgcg gtcgagccgg tgccgacgca cctctacggc gagggctaca tcgcggacaa
      961 ggccatggtc gcggagttcg agcgccgccg ccgcgacgcg gcgaaaaagc gcatcacggc
     1021 cgtcgcgcgc gaggcgcgca aggccggcgt gcctttcgag ccgctggtgc agatcgcgcg
     1081 cacgccctac gaaggcatcg tcgatgcggc gcgaaagcgc aagtgcgacc tgatcctcat
     1141 gtcctcgcac ggccaccgcg ggttctcgcg tctggtcctc ggcagcgtcg ccgacaaggt
     1201 gatccagctt tccaaggtgc cggtgctggt gtaccgctag ggacggtctg aagaagtcgc
     1261 ccccgcgaag gaacgtcacc cccgagtgat ttagtcgggg gcgggggccc agttttcttt
     1321 ctgaaaagca tgggttcccg catccgcggg aacgacggtt ggtcgccttt ttcagagttt
     1381 ctctagcgca ggaacgccgg cgccagcgcg gtcaccgcga tcaacgcagg cgtcagccag
     1441 cgcagcagcc aatgcagcgc cgaggcaccc cgcgcgccga gacgcagctc cccagcgaga
     1501 a
//
LOCUS       DATBSZ010000234         3881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1955131, whole genome shotgun sequence.
ACCESSION   DATBSZ010000234 DATBSZ010000000
VERSION     DATBSZ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1955131"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VIG70_14930"
     CDS             complement(<1..431)
                     /locus_tag="VIG70_14930"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY8251892.1"
                     /translation="MIRSALTRACEAWGLVGGIALLGAMATMVITVAGAAFGSPLLGD
                     SEIVEMLGGIAVFAFLPYCHLRGANVLVDFFTKPLPERAQRWLDAAMNAVFAAVAVIL
                     AWRLLEGGLGAYERSKRSMFLQLPDWWGYAIGMLSMLLWIAV"
     gene            complement(428..1438)
                     /locus_tag="VIG70_14935"
     CDS             complement(428..1438)
                     /locus_tag="VIG70_14935"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8251893.1"
                     /translation="MKRFAFLAVFAACAIPAQAQTVLKVHSFSGPQAPDQARHLFPWA
                     EKITKESGGKLKVEVYTNMQLGGKPADLPQQLDDGVVDAILIVPGFSPGRYPGLEGTE
                     LPFTNVGLSAGQSPAVFEWATKWLMTNELKGSRMIASHTTDASIIHSRTKPMKTLDDF
                     KGMKFRVPGRFVGEAAKALGATPVGIPLPGVYEALERGQVDGMFINWAIVPPYRLHEV
                     TKYHLETPIYQSPIMTFMSQKSYDKLPADMKKLIDANSGIEYTKKIGLVWDELTVPAK
                     KAITDRGNTLYRLDEKERQRWIAAIQPVYKIWIAEMDKRGLPGQKMFDDLLATTAKYG
                     RK"
     gene            complement(1455..2996)
                     /locus_tag="VIG70_14940"
     CDS             complement(1455..2996)
                     /locus_tag="VIG70_14940"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8251894.1"
                     /translation="MSELVLRGRHLVTDARKKASGVIADGAIAVAGGRVSDSGTFAEV
                     SARHPRARVLGNGKQLLMPGLIDAHSHGRGLSPIQKGVPTDFLENALFDWAYMPVLPP
                     ELTSGLCALRHIRSGCTTLHHNSFDDEGKAGLERAHRSVQTYLHTGIRIVFSPSIRPK
                     NRLALDDEAFWRTLPPELQEASRALVFFDRDRMEEDYFELFDSLYGRYDGEDAKILLS
                     VAWAHGTTESYLKKVRDLAGQRGKVQIHMHTLQTPVQKAYGLRAHGKPTMRWLDDLGL
                     VDRNVTYGHAIHVTEADIALIAQKGASITHHPSCNLHMRNGVAPVMQYLRAGVNVAMG
                     MDDKTINDDEDAVMEVRMIHKIHRLSSFELAEAPMDAYTALQIATTNAARVCGFENEL
                     GTLKPGMKADAILVDLERIAEDPWLDPDLDIVEAFVQRGLGSDVASAVIAGKVVMEER
                     KVVTMDVDALYREVRDFCSKGLSPEHKARADTMLRIKPYMQAWYRDWEKPVLGTPFYT
                     INSRS"
     gene            3033..>3881
                     /locus_tag="VIG70_14945"
     CDS             3033..>3881
                     /locus_tag="VIG70_14945"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335276.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY8251895.1"
                     /translation="MEMRRRSFEVTRGPALRCRGWRQEGLLRMLENVLAVGEDPENLV
                     VYAALAKAARDWESHDRIVDTLRTMSEGETLLVQSGKPIGLLKTHRQAPLVLMANCNL
                     VGQWARAEVFYELQKKNLICWGGLTAGDWQYIGSQGVIQGTYEIFARIAEQRFGGDLA
                     GRFILTAGLGGMGGAQPLAGRMANAAILCVEVDEERIERRLRSGYLDMRAQNLDEALS
                     LVKQRKPVSIALCGNAAEIYPAILERGVIPDIVTDQTSAHDLLYGYIPAGFTLEQVRA
                     MRTAEPQ"
BASE COUNT          614 a         1299 c         1290 g          678 t
ORIGIN      
        1 accgcgatcc atagcagcat gctgagcatg ccgatcgcgt acccccacca gtcgggaagc
       61 tgcaggaaca tgctgcgctt gctgcgctcg taggcgccga ggccgccctc cagcaggcgc
      121 cacgccagga tcacggcaac cgctgcgaac accgcgttca tcgccgcatc gagccagcgc
      181 tgcgcgcgct caggcagcgg cttggtgaag aagtccacca gcacgttggc gccgcgcagg
      241 tggcagtacg ggaggaaggc gaacaccgcg atgccgccga gcatctcgac gatctcggag
      301 tcgcccagca gcgggctgcc gaaggcggcg ccggcgacgg tgatcaccat cgtcgccatg
      361 gcgccgagca gcgcgatgcc gccgaccagc ccccaggcct cgcaggcgcg ggtcagcgcg
      421 ctacggatca cttcctgccg tatttcgcgg tcgtcgccag caggtcgtcg aacatcttct
      481 ggcccggcag gccgcgcttg tccatctcgg cgatccagat cttgtacacc ggctggatcg
      541 cggctatcca gcgctggcgc tctttctcgt cgagccggta gagcgtgttg ccgcggtcgg
      601 tgatcgcttt cttcgccggc acggtgagct cgtcccacac caggccgatc ttcttcgtgt
      661 actcgatgcc ggagttggcg tcgatcagct tcttcatgtc ggccggcagc ttgtcgtacg
      721 acttctggct catgaaggtc atgatcggcg attggtagat cggcgtctcg aggtggtact
      781 tcgtcacctc gtgcaggcgg tacggcggca cgatcgccca gttgatgaac atgccgtcga
      841 cctggccgcg ctcgagcgcc tcgtagacgc cgggcagcgg gatgcccacc ggcgtcgcgc
      901 cgagcgcctt ggcggcctcg ccgacgaagc gtcccggcac ccggaacttc atccccttga
      961 agtcatccag ggtcttcatc ggcttcgtgc gcgaatggat gatcgaggcg tcggtggtgt
     1021 gcgaggcgat catcctcgag cccttgagct cgttggtcat cagccacttc gtcgcccatt
     1081 cgaacaccgc cgggctctgg ccggccgaga ggccgacgtt ggtgaacggc agctcggtgc
     1141 cctcgagccc cgggtatcgc cccggcgaga atcccgggac gatcaggatc gcgtcgacca
     1201 cgccgtcgtc gagctgctgc ggcaggtcgg cgggcttgcc gccgagctgc atgttcgtat
     1261 agacctcgac cttcagcttg ccgcccgact ccttggtgat cttctccgcc cacgggaaga
     1321 gatgccgcgc ctggtcgggc gcctgcggcc cggaaaacga atgcaccttc agaacggtct
     1381 gcgcctgcgc gggtatggcg caagccgcga atacggcgag aaaagcgaac ctcttcatgg
     1441 aacctctccc ttggtcaact gcgtgaattg atggtgtaga acggcgtgcc gagcaccggc
     1501 ttctcccaat cccggtacca ggcctgcata tagggcttga tgcgcagcat ggtgtcggcg
     1561 cgcgccttgt gctcggggct caggcccttg gagcagaaat cccgcacctc gcggtagagc
     1621 gcatcgacgt ccatggtgac caccttgcgc tcttccatga ccactttgcc ggcgatcacc
     1681 gcgctcgcca cgtcgctgcc caggccgcgc tgcacgaacg cctcgacgat gtcgagatcc
     1741 gggtcgagcc acggatcttc ggcgatgcgc tccaggtcga cgaggatcgc gtcggccttc
     1801 atcccgggct tgagcgtgcc gagctcgttc tcgaagccgc acacgcgcgc cgcgttggtg
     1861 gtcgcgatct gcagcgccgt atacgcgtcc atcggcgcct cggcgagctc gaacgacgac
     1921 aggcggtgga tcttgtggat catgcgcact tccatcaccg cgtcctcgtc gtcgttgatg
     1981 gtcttgtcgt ccatccccat ggcgacgttc acgcccgcgc gcaggtactg catcaccggc
     2041 gcgacgccgt tgcgcatgtg caggttgcag ctcgggtgat gcgtgatcga ggcgcccttc
     2101 tgcgcgatca gcgcgatgtc ggcctcggtg acgtggatcg catgcccgta ggtgacgttg
     2161 cgatcgacca ggcccaggtc gtcgagccag cgcatcgtcg gcttgccgtg ggcgcgcagg
     2221 ccgtaggcct tctgcaccgg ggtctgcagg gtgtgcatat ggatctggac ttttcctcgc
     2281 tggcccgcca ggtccctgac ttttttcagg tacgactcgg tggtcccatg tgcccacgcc
     2341 accgacagca ggatcttggc gtcctcgccg tcgtagcggc cgtagaggct gtcgaagagc
     2401 tcgaagtagt cctcctccat gcgatcgcgg tcgaagaaca ccagcgcgcg gctcgcctcc
     2461 tggagctcgg gcggcagcgt gcgccagaac gcctcgtcgt cgagcgcgag gcggtttttc
     2521 gggcggatcg aaggcgagaa cacgatgcgg atcccggtgt gcaggtaggt ctgcacgctg
     2581 cggtgcgcgc gctcgagccc cgccttgcct tcgtcgtcga agctgttgtg gtgcagcgtg
     2641 gtgcatcccg aacggatgtg gcgcagcgcg cacagccccg aggtgagctc gggcggcagc
     2701 accggcatgt acgcccagtc gaacagcgcg ttctcgagaa aatcggtcgg cacgcccttc
     2761 tggatcggcg acaggccgcg gccgtggctg tgcgcgtcga tgaggcccgg catcaggagc
     2821 tgcttgccgt tgccgagcac gcgcgctcga ggatggcgcg cactgacttc agcgaacgtt
     2881 ccgctgtccg agaccctgcc gcccgcgacc gcgatggcac cgtcggcgat taccccggac
     2941 gcttttttcc tggcgtcggt caccagatgg cgcccgcgca gcaccagctc gctcatgcgg
     3001 cgagtgtagc aaaccgacta gaatcgaaac gcatggagat gcgcaggcga tcgttcgagg
     3061 tcacgcgcgg ccccgctctg cgctgccgcg gctggcggca ggaaggcctg ctgcgcatgc
     3121 tggagaacgt gctcgccgtc ggcgaggatc ccgagaacct cgtggtgtac gcagcgctcg
     3181 ccaaggcggc gcgcgactgg gaatcgcacg accgcatcgt cgacaccctg cgcaccatgt
     3241 ccgagggcga gacgctgctg gtgcaatcgg gcaagccgat cggcctgctc aagacgcacc
     3301 gccaggcgcc gctcgtgctg atggcgaact gcaacctcgt cgggcagtgg gcgcgcgccg
     3361 aggtgttcta cgagctgcag aaaaagaacc tgatctgctg gggcggcctg accgcggggg
     3421 actggcagta catcggctcg cagggcgtga tccagggcac ctacgaaatc ttcgcgcgca
     3481 tcgccgagca gcgcttcggc ggcgacctcg cgggacgctt catcctcacc gccgggctgg
     3541 gcggcatggg cggcgcgcag ccgctcgccg ggcgcatggc caacgccgct atcctgtgcg
     3601 tcgaggtcga cgaggagcgc atcgagcggc gcctgcgctc gggttatctg gatatgcgcg
     3661 cccagaacct ggacgaagca ctgagtctgg tgaagcaaag aaaacccgtg tcgatcgcac
     3721 tatgcggcaa cgcggccgag atctatcccg cgatcctcga gcgcggcgtg attcccgaca
     3781 tcgtgaccga ccagacctcg gcgcacgacc tgctctacgg ctatatcccg gcgggcttca
     3841 cgctcgagca agtgcgcgcg atgcgcacgg ccgagccgca g
//
LOCUS       DATBSZ010000235         8143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_112074, whole genome shotgun sequence.
ACCESSION   DATBSZ010000235 DATBSZ010000000
VERSION     DATBSZ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8143
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112074"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VIG70_14950"
     CDS             complement(<1..881)
                     /locus_tag="VIG70_14950"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEY8251896.1"
                     /translation="MKVSSVLVVGGSGFIGRHLVASLAAAGISVTVPSRRRERAKHLI
                     LLPTVDVVEADVMAPGTLERLCERKDAVYNLAGVLHSRRGRSHERGANEYGPDFARIH
                     VELPQGIVAACRIAGVRRVLHVSALGASPGAPSEYLRSKGIGEQAVLAAEDLDVTVFR
                     PSVVFGPEDAFLNRFAGMTRFLPVMAVPCPEAKFQPVYVGDVARALHFALEEPEARGK
                     AYDLCGPRVYTMRELVESVCAMTGRNRLVVGLPDRLSYLQAWMLEHLPGKLMTRDNYR
                     SMQVPNTCDKPFPFQLQP"
     gene            complement(944..2908)
                     /locus_tag="VIG70_14955"
     CDS             complement(944..2908)
                     /locus_tag="VIG70_14955"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY8251897.1"
                     /translation="MISKVFRNLAVGLACVLAAGAALAKDSGKNSADESLLAAYDAYR
                     AGDPIKLSRHAKKIEGHVLEPWLDYWRLSMRLEDVPNREVREFLATHADLYVNERLRS
                     DWLRVLGKRSEWPEFDRQALRYTRDDLEVSCYRWISRLEHGDDSALAEATAMWLEPTE
                     LPDGCQRLSAILSARGRLSVTDVWRRVRVLFEHGQITAAKTALALLPKNEAPDERMLA
                     EAARQPKRFLERLPKALETRAAHEVAVLGAIRYARLDPAGTAAALAGPLAERLSESEL
                     RYVWGVVGYEGAREHHDDALNWFARAGDTPLEDRHLAWKARAALRDGEWPVVRESIDR
                     MSLAAAHQSAWIYWYGRALSRQGEEMGSRAYFLRIAGQADFYGLLASEELGYVMGLPD
                     VTHVPSEQEVAAAAEEPGLKRALELIRLGIRVEGVREWLFTIRSFDDARLLAAAELAR
                     RDGVYDRAIHTADRTSRLHNFTLRYPMPYQDVFRGYAATHGVDEAWVLAVVRQESRFI
                     TDARSSAGAAGLMQVMPGTARYVAAKIGLRNYRPKAVTNLETNVTLGTGYLKLVMEQL
                     GHPVLASAAYNAGPKRARRWRDDKPLEGAIYIESIPFSETRDYVKKVMSNAVYYSAVL
                     EKKPVPLKSRLGVIQPPGSSEPPVFEDEAR"
     gene            2926..3600
                     /locus_tag="VIG70_14960"
     CDS             2926..3600
                     /locus_tag="VIG70_14960"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY8251898.1"
                     /translation="MDATTLKAWRRAERERLIAARSAVAPAVLDSWRRRIDSFLERSF
                     PGLAHCRLAFCWPIKGEYDARHLARTLRSRGALTGLPVVVAPKTPLIFREWHPGIALA
                     KGALDIPYPVGSQELTPEAVLLPMNGWDAKGYRLGYGAGFFDRTLASLARKPLTIGIS
                     YELAKMPSIHPQDWDLPMDYVVTERGVYRRDPEGLVFLGEPDQGSGTLSSPVCYADED
                     YFSGKK"
     gene            complement(3601..4248)
                     /locus_tag="VIG70_14965"
     CDS             complement(3601..4248)
                     /locus_tag="VIG70_14965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8251899.1"
                     /translation="MAGRAPLLPEKIARYIHEHAVREPQVLRELRAATAAVPYSGMQI
                     GADQGQFMAMLVRLMGAKRCLEIGTYTGYSALAVALALPKDGSIVCCDVSEEWTAVGK
                     PFWKKAGVEKKIDLRIGPALETLKKLKGPFDFVFIDADKPNYLNYYERCLKLVRRGGL
                     IAVDNVLWSGEVANDKARDELTVALRKFNDRVHKDKRVDLAMLSIGDGVTLALKK"
     gene            complement(4252..5763)
                     /gene="ilvA"
                     /locus_tag="VIG70_14970"
     CDS             complement(4252..5763)
                     /gene="ilvA"
                     /locus_tag="VIG70_14970"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEY8251900.1"
                     /translation="MNKNYLERILKARVYDVASESPLELAPGLSKRLGNRLYLKREDL
                     QAVFSFKLRGAYNKMAGLPRQRLARGVIAASAGNHAQGVALAAQRLGCRAVIVMPITT
                     PRIKVDAVAARGAEVVLHGDSYSEAYAKAMQIKKKRGLAFVHPYDDPDVIAGQGTIGM
                     EILRQHPSTIDAIFVPVGGGGLISGIASYVKRVRPAVKVIGVEPVDADAMTRSLKAGR
                     RIKLDHVGLFADGVAVKEVGRETFRLCRSLVDEMVLADTDAMCAAIKDVFEDTRVVLE
                     PAGALATAGAKAWVERHGVRGKALVAVASGANTNFDRLRFIAEEAELGEHREAILAVT
                     IPERPGSFRKFCALLGARNVTEFNYRIADTRDAHIFVGIEVQGREETARIVRNLRRHG
                     LTTLDLSDNDMAKLHTRHMVGGRAPFARDELLYRFEFPERPGALMRFLNSMRSDWNIS
                     LFHYRNQGADYGRVLVGMQVPRKEMSQFRAFLKKLGYPYADETRNSAYKLFLR"
     gene            5781..6167
                     /locus_tag="VIG70_14975"
     CDS             5781..6167
                     /locus_tag="VIG70_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY8251901.1"
                     /translation="MTELLGLLAASFLAATLVPLPSEAALFAYLQLHPDRAALAVGVA
                     TLGNTAGGMTSYLFGRLIPQKKLNLKAVEWLRRYGAPVTVLAWLPLVGDALCVAAGWL
                     RINWIAALGFMAAGRLGRYALVAALS"
     gene            6172..6597
                     /locus_tag="VIG70_14980"
     CDS             6172..6597
                     /locus_tag="VIG70_14980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEY8251902.1"
                     /translation="MQIRPFEREDEPAVVALWEACGLTRPWNDPHRDIARKLAVQPEL
                     FLVGVVERAVIATVMAGYEGHRGWVNYLAVAPGVRGRGFARALMLHVEALLLGQGCPK
                     VNLQVRTSNAEAIAFYHHLGYVQDEAVSLGKRLIPDALG"
     gene            6584..6967
                     /locus_tag="VIG70_14985"
     CDS             6584..6967
                     /locus_tag="VIG70_14985"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEY8251903.1"
                     /translation="MRWAERARRLKTELFALYLAARHPQTPWYAKLAVAGFVAYAVTP
                     VDLVPDAFPILGWIDDLIFVPIAIALAVRFVPPPVLAECRLRAQERALKQSKSGWWII
                     VAVWITLAGAAIVLHYNGFFAVINR"
     gene            6992..7981
                     /locus_tag="VIG70_14990"
     CDS             6992..7981
                     /locus_tag="VIG70_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8251904.1"
                     /translation="MKILPIVSFVGVALAAAPVLAQQRIVKIDGSSTVFPVTEAVAED
                     FQKAKKQQVKVTVGISGTGGGFKKFCRGETDISNASRPILKAEMADCAKAGIEYIELP
                     VAFDALTVVINPKNTFIKQLTIAEMKKMWEPAAQGKVTRWNQVNPQWPDAPMKLFGPG
                     ADSGTFDYFTEAIVGKSKSSRGDFTASEDDNVLVQGVSRDVNGLGYFGYAYYIENKDK
                     LKAVPIVNDKGQAVEPSMEAVLKGSYTPLARPIFIYVNARSFAKPEVKEFVEFYMKNG
                     AKLAKEVKYVPLPDSAYKTALEHAQKGKKGTVFGGTAEVGVTIDELLKREAKL"
BASE COUNT         1291 a         2747 c         2738 g         1367 t
ORIGIN      
        1 ggctggagct ggaacggaaa cggcttgtcg caggtgtttg gcacctgcat cgagcggtag
       61 ttgtcgcgcg tcatcagctt cccgggcagg tgctcgagca tccacgcctg caggtacgag
      121 agccggtcgg gcagccccac cacgaggcga ttcctgcccg tcatcgcgca aacggattcg
      181 acgagctccc tcatcgtgta gacgcgcggc ccgcagaggt cgtaggcctt gccgcgcgcc
      241 tcgggctcct cgagcgcgaa gtgcagcgcg cgcgcgacgt cgccgacgta gaccggctgg
      301 aacttcgctt ccggacacgg gaccgccatg acggggagaa accgcgtcat cccggcaaac
      361 cggttcagaa acgcgtcctc gggcccgaac accaccgaag ggcgaaacac cgtcacgtcg
      421 aggtcctcgg cggcgagcac cgcctgctcg ccgatgccct tcgagcgcag gtactcggaa
      481 ggcgcgccgg gcgacgcgcc gagcgcgctc acgtgcagca cgcgccgcac gccggcgatg
      541 cggcacgctg cgacgatgcc ctgcggcagc tcgacgtgga tgcgcgcgaa atccggaccg
      601 tattcgttcg cgccgcgctc gtggctccgc ccgcgccggc tgtgcagcac gccggccaag
      661 ttgtagaccg cgtccttgcg ctcgcagagg cgctcgagcg tgcccggggc catgacgtcg
      721 gcctcgacca cgtccactgt cggaagcagg atgaggtgct tcgcgcgctc gcgccggcgc
      781 gagggcacgg tgacgctgat tccggcggcg gcgagcgagg cgacgaggtg gcggccgatg
      841 aagcccgagc cgccgacgac caggaccgaa ctgactttca cacccccgat tctacgggag
      901 cttgactggg tccccgcctg cgcggggacg accattcagc acttcagcgc gcttcgtcct
      961 cgaataccgg cggctcggag gacccgggcg gctggatcac gccgagcctc gacttgagcg
     1021 gcaccggctt cttctccagc accgcggagt aatagaccgc gttcgacatc accttcttca
     1081 catagtcccg cgtctcggag aacggaatcg actcgatata gatcgcgcct tcgagcggct
     1141 tgtcgtcgcg ccagcgccgc gcgcgcttcg ggccggcgtt gtaggcggcc gaagcgagca
     1201 ccgggtggcc gagctgctcc atcaccagct tgagataccc cgtgccgagc gtcacgttgg
     1261 tctcgaggtt ggtcacggct ttcggccggt agtttcgcag gccgatcttc gccgcgacat
     1321 agcgcgcggt tcccggcatg acctgcatca ggcccgcggc gccggcgctc gagcgcgcat
     1381 cggtaatgaa gcggctttcc tggcgcacca cggcgagcac ccacgcctcg tcgacgccgt
     1441 gtgtggcggc gtagccgcgg aacacgtcct ggtacggcat cggatagcgc agcgtgaagt
     1501 tgtgcagccg cgaggtgcgg tcggcggtat ggatcgcgcg gtcgtagacc ccgtcgcggc
     1561 gcgcgagctc cgcggcggcg agcaggcgcg cgtcgtcgaa agagcggatg gtgaagagcc
     1621 actcgcgcac gccttcgact cggatgccga ggcgaatgag ctccagcgca cgcttgagtc
     1681 cgggctcctc ggcggccgcg gcgacttcct gctcgctcgg gacgtgcgtc acgtcgggca
     1741 gccccatcac gtagccgagc tcctcggagg cgagcaggcc gtagaagtcc gcctgtccgg
     1801 cgatgcgcag gaaataggcg cggctgccca tctcctcgcc ctggcgggag agcgcgcgcc
     1861 cgtaccagta gatccacgcc gactggtgcg cagccgctaa cgacatgcgg tcgatcgatt
     1921 cacgcaccac cggccactcg ccgtcgcgca gcgcggcgcg cgccttccac gcgaggtggc
     1981 ggtcttcgag cggcgtgtcg cccgcgcgcg cgaaccagtt gagcgcgtcg tcgtgatgct
     2041 cgcgcgcccc ttcgtagccg acgacgcccc agacgtacct cagctcggat tcggagagcc
     2101 gctcggcgag cggccctgcg agcgcggcgg cggtgccggc cgggtcgagg cgcgcatagc
     2161 gaatcgcgcc gagcaccgcg acctcgtgcg ccgctcgggt ctcgagggcc ttgggcagtc
     2221 gctcgaggaa gcgcttgggt tggcgcgccg cctcggcgag catgcgctca tcgggcgcct
     2281 cattcttggg caggagcgca agcgcggtct tcgcagcggt gatctggccg tgctcgaaca
     2341 gcactcgcac gcggcgccat acgtcggtga ccgacaggcg gccgcgcgcc gaaaggatcg
     2401 cgctcagccg ctggcagccg tcggggagct cggtaggctc gagccacatc gccgtcgctt
     2461 cggcgagcgc gctgtcgtcg ccgtgctcga ggcgcgagat ccaccggtag cagctcacct
     2521 cgaggtcgtc gcgcgtgtag cgcagggcct ggcggtcgaa ctccggccac tcgctgcgct
     2581 tgccgagcac gcgcagccag tcgctgcgca agcgctcgtt cacgtacagg tcggcgtgcg
     2641 tggcgaggaa ctcgcgcacc tctcggttgg gcacgtcctc cagccgcatc gagagccgcc
     2701 agtagtcgag ccagggctcg aggacatggc cttcgatctt cttggcgtgc cgcgataact
     2761 tgatcgggtc gccggcgcgg taggcgtcgt aggccgcgag cagcgactcg tcggccgaat
     2821 tcttcccgga gtccttggca agcgcggcgc cggcggccag tacgcaggcc agccccaccg
     2881 cgaggttgcg gaaaacctta gaaatcataa aactaggcta cccccatgga tgccaccaca
     2941 ctaaaagcct ggcgtcgggc cgagcgcgag cggctgatcg ccgcccgttc tgcagtcgct
     3001 cccgcggtgc tcgattcatg gcgccgacgg atcgacagct tcctcgagcg ctcattcccc
     3061 ggccttgccc actgccgcct cgccttctgc tggccaatca agggcgaata cgacgcccgc
     3121 catcttgcca gaacgctgcg ctcccgtgga gcgttgaccg gactgccggt cgtcgtagcg
     3181 ccgaaaactc cgctcatttt ccgcgaatgg caccccggga tcgcgcttgc caagggtgcg
     3241 cttgacattc cgtacccggt gggctcacaa gaattgacac ccgaggcggt tctcctgccc
     3301 atgaacggct gggatgcgaa gggctatcgg ctgggctacg gcgccggatt cttcgaccgc
     3361 acactcgcga gccttgccag gaaaccgctc acgatcggca tctcctatga actggcgaag
     3421 atgccgtcga tccacccgca ggactgggac ctgcccatgg actacgtggt caccgagcgc
     3481 ggcgtctatc ggcgcgaccc ggagggactg gtctttctcg gcgagcccga tcaaggctcg
     3541 gggacccttt cctcgccggt ctgctacgcg gatgaggatt atttcagcgg caagaaatag
     3601 ctatttcttg agcgccagcg tgaccccgtc gccgatggaa agcatcgcga ggtccacgcg
     3661 cttgtccttg tgcacccggt cgttgaactt gcgcagggcg acggtgagct cgtcccgcgc
     3721 cttgtcgttc gccacttcgc ccgaccacag cacgttgtcg actgcgatca ggcccccgcg
     3781 ccgcacaagc ttcaggcagc gctcgtagta gttgaggtag tttggcttgt cggcgtcgat
     3841 gaagacgaag tcgaacgggc ccttcagctt cttgagggtc tccagggcgg ggccgatcct
     3901 gagatcgatc tttttctcga cgcccgcttt cttccagaaa ggcttgccga cggcggtcca
     3961 ttcctcgctc acgtcgcagc acacgatgct gccgtccttc ggcagcgcga gcgcgacggc
     4021 gagcgcgctg tagccggtgt aggtgccgat ttcgaggcag cgcttcgcgc ccatcagcct
     4081 caccagcatc gccatgaact ggccctggtc ggcgccgatc tgcatcccgg agtacggcac
     4141 cgcggcggtc gcggcgcgca gctcgcgcag gacctgcggc tcgcgcaccg catgctcgtg
     4201 gatgtagcgc gcgatttttt ccggcagcag gggggcgcgt ccggccatgg cctatcgcag
     4261 gaacagcttg tacgcggaat tgcgcgtctc gtcggcgtac ggatagccga gctttttcag
     4321 aaaagcgcga aattgagaca tttcttttcg cgggacctgc atccccacga ggacgcggcc
     4381 gtagtcggcg ccctggttgc ggtagtggaa caggctgatg ttccagtcgc tgcgcatgct
     4441 gttgaggaac cgcatcagcg cccccgggcg ctccgggaac tcgaaccggt agaggagctc
     4501 atcccgggcg aacggcgcgc ggccgccgac catgtggcgc gtgtgcagct tggccatgtc
     4561 gttgtcggag aggtcgagcg tggtgagccc gtgccggcgc aggttgcgca cgatgcgcgc
     4621 ggtctcctcg cgcccctgca cctcgatgcc cacgaagatg tgcgcatcgc gcgtgtcggc
     4681 gatccggtag ttgaactcgg tgacgttgcg cgcgccgagc agcgcgcaga acttccggaa
     4741 gctgcccggc cgctccggga tcgtgaccgc gaggatcgcc tcgcggtgct cgccgagctc
     4801 ggcctcctcg gcgatgaaac gcaggcggtc gaagttggtg ttcgctcccg aggccaccgc
     4861 gaccagcgcc ttgccgcgca cgccatggcg ctccacccac gccttcgccc ccgcggtcgc
     4921 cagggccccg gcgggttcca gcaccacgcg cgtgtcctcg aatacgtcct tgatcgccgc
     4981 gcacatcgcg tcggtgtcgg cgagcaccat ctcgtcgacc aggctgcggc acaggcggaa
     5041 ggtctcgcgc cccacctcct tcaccgcgac gccgtccgcg aacaggccca cgtggtcgag
     5101 cttgatcctt cttcccgcct tgagcgagcg agtcatcgcg tccgcgtcga cgggttccac
     5161 gccgatcact ttgacggcgg gccggacccg tttcacgtac gaagcgatgc cggagatgag
     5221 cccgccgccg ccgaccggga cgaagatcgc gtcgatcgtg ctcggatgct ggcgcaggat
     5281 ctccatgccg atggtcccct ggccggcgat tacgtcgggg tcatcgtatg gatgcacgaa
     5341 ggcgagaccg cgcttcttct ttatctgcat ggccttcgcg tacgcctcgg agtacgagtc
     5401 gccgtgcagc accacctcgg cgccgcgcgc cgccaccgcg tcgaccttga tgcgcggcgt
     5461 agtgatgggc atcacgatca ccgcgcggca gcccaggcgc tgcgccgcga gcgcgacgcc
     5521 ctgcgcatgg ttccccgcgc tcgccgcgat caccccgcgg gcgagccgct ggcgcggcaa
     5581 tcccgccatc ttgttgtagg cgccgcgcag cttgaacgag aataccgcct gcaggtcctc
     5641 gcgcttgagg tacaggcggt tgccgaggcg cttcgacagg ccgggcgcga gctcgagcgg
     5701 cgactcgctc gccacatcgt agacccgcgc cttcaggatt ctttcgagat agttcttgtt
     5761 caagagccgc cgaatttagc atgacggaac tgctcggcct cctcgccgcg agcttcctcg
     5821 cggccacgct cgtcccgctg ccttcggagg cggcgctctt cgcctacctg cagcttcatc
     5881 ccgaccgcgc cgcgctcgcg gtcggcgtcg cgacgctcgg caacaccgcc ggcggcatga
     5941 cttcctattt gtttggcagg ctgatccctc aaaaaaaact gaatctgaag gcggtcgagt
     6001 ggctgcgccg ctacggcgcg ccggtcacgg tgctcgcctg gctgccgctc gtcggcgacg
     6061 cgctgtgcgt ggccgcgggt tggctgcgga taaactggat cgccgcgctg ggcttcatgg
     6121 ctgccggccg actggggcgc tacgcgctgg tagcggcgct atcctgagct gatgcagatc
     6181 cgtcccttcg agcgggagga cgagccagcc gtggtcgcgc tctgggaggc gtgcgggctg
     6241 acgcggccgt ggaacgatcc gcacagggac atcgcgcgca agcttgcggt gcagccggag
     6301 ctttttctgg tcggcgtcgt cgagcgcgcc gtgatcgcga cggtcatggc gggatacgaa
     6361 ggccatcgcg gctgggtgaa ttaccttgcg gtcgcgcccg gcgtccgcgg ccgcggattc
     6421 gcgcgtgctc taatgctgca tgtggaagcg ctgctcctcg gccaggggtg tccgaaggtg
     6481 aacctgcagg tccgcacctc gaacgccgag gcgatcgcgt tctatcacca tctcggctat
     6541 gtccaggacg aagccgtcag cctcggcaag cggctgattc ccgatgcgct gggctgaacg
     6601 cgcgcgacgc ctgaagaccg agctgttcgc gctctacctc gccgcgcgcc atccgcagac
     6661 gccgtggtac gcgaagctgg ccgtggcggg cttcgtcgcc tatgccgtga cgccggtgga
     6721 cctcgtgccg gatgcgtttc cgatcctcgg ctggatcgac gacctgatct tcgtgcccat
     6781 cgcgatcgcg ctcgcagtgc ggttcgtacc ccctcccgtg ctagccgaat gccgcttgcg
     6841 agcgcaggag cgcgcgctca agcagtcgaa atcgggctgg tggatcatcg tggcggtctg
     6901 gataaccctt gccggggcag ccatcgtgct ccattacaac gggttttttg cagtcataaa
     6961 tcggtaatgc ccgctgcgta ggctgcgcgg catgaaaatc ctacctatcg tgtcgtttgt
     7021 cggcgtggcg ctggccgccg cgccggtcct cgctcagcag cggatcgtca agatcgacgg
     7081 ctcgagcacc gtgttcccgg tgaccgaggc ggtcgccgag gacttccaga aggcgaagaa
     7141 gcagcaggtc aaggtgacgg ttggcatctc cggcaccggc ggaggcttca agaagttctg
     7201 ccgcggcgag accgacatct cgaacgcctc gcggccgatc ctgaaggcgg agatggccga
     7261 ctgcgccaag gcgggcatcg agtacatcga gctgccggtg gcgttcgacg cgctgacggt
     7321 ggtgatcaac ccgaagaaca ccttcatcaa gcagctcacc atcgccgaaa tgaagaagat
     7381 gtgggagccc gcggcccagg gcaaggtgac gcgctggaac caggtgaatc cgcagtggcc
     7441 cgacgcgccg atgaagctgt tcgggccggg ggcggactcg ggcaccttcg attacttcac
     7501 cgaagcgatc gtcggcaagt ccaagtcctc gcgcggcgac ttcaccgcct ccgaggacga
     7561 caacgtcctg gtgcagggcg tgtcgcgcga cgtcaacggc ctgggctact tcggctacgc
     7621 gtattacatc gagaacaagg acaagctgaa ggcggtgccg atcgtgaacg acaagggcca
     7681 ggcggtcgag ccgtcgatgg aggcggtgct gaagggcagc tacacgccgc tcgcacggcc
     7741 cattttcatc tatgtgaacg ccaggtcgtt cgccaagccc gaggtgaagg agttcgtcga
     7801 gttctacatg aagaacggcg ccaagctggc gaaggaagtg aagtacgtgc cgctgccgga
     7861 ctcggcctac aagaccgcgc tcgagcacgc gcagaagggc aagaagggca ccgtgttcgg
     7921 cggcacggcg gaagtcggcg tgacgatcga cgagctgctg aagcgcgaag ccaagctctg
     7981 aagccagctt cgctttcgct agaatcgggg ccgatgcaga ccccgcaagc cgcgcgcctc
     8041 gcgcggcgac ttcaccgcct ccgaggacga caacgtcctg gtgcagggcg tgtcgcgcga
     8101 cgtcaacggc ctgggctact tcggctacgc gtattacatc gag
//
LOCUS       DATBSZ010000236        10098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1818858, whole genome shotgun sequence.
ACCESSION   DATBSZ010000236 DATBSZ010000000
VERSION     DATBSZ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10098
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818858"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /locus_tag="VIG70_14995"
     CDS             <1..1538
                     /locus_tag="VIG70_14995"
                     /inference="COORDINATES: protein motif:HMM:NF025464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY8251905.1"
                     /translation="YDSAGAANDTSVYFYVQNGTTPPPTPPANQPPTVSFKVPKNGQQ
                     VSGTLNDSTCEALATDDKGVKQVQFFLGSTALGTEFGAPYTCGVDTKKFANGTHTLKA
                     VATDTNGASTTSQISINIQNLVANTPPTVALTAPAAGASLSGTAVGYGASASDPGGSV
                     AKVEFYLAPGTQTAGPLAPFATKTSAPYAGTLDTSKVANGSYTLMAVATDNLGAAATT
                     QRPVTVSNVVAPPAPTPVATADILTRASADMPFAQQSGYTAQVINTYTSASAIPESGI
                     RGSALPDGDTMRFGKVADPTSSALKALAFQVTSTDPTTSGGKRTELSIAPNIQHDKVY
                     WIAFSAYVHDWGTLGANDLALFGTQLHQGNNQLKVGGPAFGLYTKQNGRSFRVRGRYS
                     EAATPSESNAYNVEFTDYPIPFGRWTDFVLKFKQNTSGNGLLQVWMDGVQIADYHGSL
                     GYNTGYNDYIKFGYYNWSLTTMSSTPRKVLLRSPTIVADPTGSKYQPEQLRALVSADA
                     AAQ"
     gene            1550..1927
                     /gene="gspG"
                     /locus_tag="VIG70_15000"
     CDS             1550..1927
                     /gene="gspG"
                     /locus_tag="VIG70_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEY8251906.1"
                     /translation="MLKPHRGFTLLELLVVIVIIGMLAGYVAPRYFSHVGKSETQVAR
                     AQIDALEKALDTYRLDNRRYPSAEEGLAAIGPYLKKAVPNDPWGRPYVYRTPGQRGEY
                     EVFSYGRDGKAGGTGEDADIGTS"
     gene            1930..3111
                     /locus_tag="VIG70_15005"
     CDS             1930..3111
                     /locus_tag="VIG70_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8251907.1"
                     /translation="MQFNVLAVDSRQQVVALDLEAASEALAADQARARGLSVVSLERK
                     GFALPKRKARFPATLFSMELLSLLQAGLNLVEALQTLSEKESGGERTEVLSAILAAIH
                     RGEPFSQAVAALPRHFSPLYVATIKAAERTGNVPEALGRYIAYQEELDRVRKKIVSAS
                     IYPAILVVVGGLVLAFLMFYVVPRFARVYEDMAGTLPLFSKLLLAFGGFVGNNVFVLV
                     GAFCGLVAGAVWAWSRPEVRSWLNTQVWRVPALGKRMKVYQLARLYRTTAMLLRAGIP
                     AVRALDMVRDLLAAHLRPQLMKAKKSIEQGVAMSAALGAVGLATPVAARMMVVGERSG
                     DMGEMLSQIARFHDDEIARTVEWFTKAFEPILMAVLGLAIGGVVVLMYMPIFELAGSI
                     R"
     gene            3108..4622
                     /locus_tag="VIG70_15010"
     CDS             3108..4622
                     /locus_tag="VIG70_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY8251908.1"
                     /translation="MKMETTVVHTLAELSSRLRMPCASIEEMRESRAAFDLVPYAEAS
                     RRACVALRAADGAVSIVLAEPFDLDTQDWLEERLGVPFHYRLASRADVEAYLAQQETG
                     LRAMDGVSKDLRADTRSDGAHEISFEAAADADSTVVRLVSSTLYDALKAGASDVHLES
                     TASGLSIKYRVDGVLTSAQTMPGTELAEQVISRIKVMSELDIAERRVPQDGRFKARHR
                     NGADAGRDIDFRVSVMPSVFGEDAVLRILDRRALTDQLQGLSLEVLGFEPGLRARLSD
                     LSKEPYGMLLVTGPTGSGKTTTLYAVISEANKGLDKIVTIEDPVEYQLPGVLQIPVNE
                     QKGLTFARGLRSILRHDPDRIMVSEIRDPKTAEIAMQSALTGHLVYTTVHANNAIDVL
                     GRFQHMGVDTYNLVSALNGVLAQRLVRVLCKTCRSRGCADCRGTGFKGRKAIGELVVL
                     NDELRELIVARAPARKLKDAVRAAGTVALREAAMALVQAGETTLEEINRVTFVA"
     gene            4603..5346
                     /locus_tag="VIG70_15015"
     CDS             4603..5346
                     /locus_tag="VIG70_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251909.1"
                     /translation="MSLSWLKRKHLRIGLGAKRVMVSGAKAVELPAADDWRAALEALP
                     EILKSHKSREASVVLADQFVRYALLPWNEAVKSPAQWLALARHRFGALHGTAAAEWNV
                     KVTDTAPRGPRLACAVDGGLIEGLACAFVGASVTLVSVQPFLVAAFNRIRQMVGNASC
                     WIVVEEPGRLTLALIQRGAWVAIRSRRSDERWRVALPEILEREGAFLGLNEACTRVIV
                     CAQGEFDPQMHDAWRAHAMSYRELALAWE"
     gene            5346..5846
                     /locus_tag="VIG70_15020"
     CDS             5346..5846
                     /locus_tag="VIG70_15020"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEY8251910.1"
                     /translation="MKKIQIDFGSAGRGSPWVGRALLAIAAVVCVDAAFTYQGLHQSV
                     KENGARLAKRSSTGAVAKVSPQEVAAVRETVERIALPWDQLFSALESAASDKVALAAI
                     EPDTAKGTVTISGESKDYLAALSYVLNLGRSDSLDHVQLVRHEPKANDPNGAVSFAVS
                     AVWSAK"
     gene            5843..6349
                     /locus_tag="VIG70_15025"
     CDS             5843..6349
                     /locus_tag="VIG70_15025"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HEY8251911.1"
                     /translation="MKFSLKPVQERIGVTGLAAIALLAAALAFSNLVVKPLKEKSALL
                     QDRVSRQAPDGARPSADKVAAVYQFLEKEEQATDWLAKLHGIGAATGVQLKSASYRTQ
                     KTDARIVRYEMVLPVAGSYAQIREFLQRSLAEVPVLSVDQLNLKRDGKNANVIQAEMR
                     LTLHMVKS"
     gene            6352..6834
                     /locus_tag="VIG70_15030"
     CDS             6352..6834
                     /locus_tag="VIG70_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251912.1"
                     /translation="MDKRHIVLGIVAVTLVAGVVAGRERPAVELAQPRAPQAAAAKYD
                     DIDLDKLRRAEVRAPSTDPFAAMKGPEIRAAAAAAAPEAPARPTAPPLPFQYIGKWSQ
                     GDKGEVLLQRGEELLPIAPGAKLGDYRVDQITEARVSFTYLPLNMKQTLDLLGPEARQ
                     "
     gene            6831..7274
                     /locus_tag="VIG70_15035"
     CDS             6831..7274
                     /locus_tag="VIG70_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8251913.1"
                     /translation="MKGFTLIELLIVVAIVALLASIAAPLAELGYQRGKEQELRYALR
                     EIREAIDAYKRASDEGKIERKADASGYPPSLATLVEGVEEKTNPEKTKIYFLRRIPKD
                     PLGGEAWGLRSYASPADDPRDGKDVYDVYSRSEGVGLNKLPYREW"
     gene            7283..7642
                     /locus_tag="VIG70_15040"
     CDS             7283..7642
                     /locus_tag="VIG70_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8251914.1"
                     /translation="MTLKKRLGFTLVELMVVLTVIALLLSVVVPDYVGRMKRAEESAL
                     QENLAVMRDALDKHYADAGKYPATIEELVSKRYLRSIPRDPFTQKADTWVAVPPADPK
                     SGGVFDIHSAAKGYERW"
     gene            7797..8261
                     /locus_tag="VIG70_15045"
     CDS             7797..8261
                     /locus_tag="VIG70_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8251915.1"
                     /translation="MGFLLAVAIMGAGLVAYSELASHAAQREKEAELLFRGNQYRAAI
                     GSYYKKEQRFPKSLADLLEDKRYPMPVRHLRKLYPDPVAGTPAWGLIEAPEGGIMGVY
                     SLSEEAPIKIGNFAARDAALAEAPRTAQSGEPRYADWKFFYRPSPPGPKAAG"
     gene            8293..9360
                     /locus_tag="VIG70_15050"
     CDS             8293..9360
                     /locus_tag="VIG70_15050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8251916.1"
                     /translation="MSEGSIAVGARASPLRWLFGELPCARPAGRLLDVDAARGLAIIL
                     VVVGHVGARVATLEDAGWYADLKAAIYLFHMPLFMALTGITFALSLPAFTAWSEAARF
                     SFKRVERLLVPYVVFGLVVILGKLAASRVMHVDRAPQDLAGELYLMLVMPGSSVTVFI
                     WFIYVVSIYLLLVPALFRLIGRRPMLLFIASLVLQLGEWPQLFMLDRVVEYLPFFAGG
                     MLLWMHRAAWLRIMPAVLWPATIVFVALLVLSLWLKLPKWLVGAVSVPAVLAWMHLLA
                     AQPQGWLAALGRASLAIYLMNTIAIGLVKGVMLKFLPWHGVNFLLYFPLLTLAGIALP
                     LLVASFVKRHAPRAGRYLGTA"
     gene            complement(9574..9864)
                     /locus_tag="VIG70_15055"
     CDS             complement(9574..9864)
                     /locus_tag="VIG70_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251917.1"
                     /translation="MVWNRVEEPHAVCESLSGRKNFFNILGCSHRSEPAAEGAPRVCS
                     IYAPAPRNERDLQRFATLGHELMHCFDGNWHDRWGRMNSPAETAVAGGSASP"
BASE COUNT         1691 a         3436 c         3374 g         1597 t
ORIGIN      
        1 cgtacgacag cgccggcgcg gcgaatgaca cgtcggtcta cttctacgtt cagaacggca
       61 ccaccccgcc gcccactccg ccggccaacc agccgccgac ggtgagcttc aaggtaccga
      121 agaatggtca gcaggtgtcc ggcacgctca acgactcgac gtgcgaggcg ctcgccaccg
      181 acgacaaggg cgtgaagcag gtccagttct tcctcggctc gaccgcgctc ggcaccgagt
      241 tcggcgcgcc gtatacctgc ggcgtcgata cgaagaagtt cgccaacggc acgcacacac
      301 tgaaggcggt cgccaccgac acgaacgggg cgagcaccac gtcgcagatc agcatcaata
      361 tccagaacct cgtggcgaac acgccgccca ccgtggcgct cactgccccg gccgccggcg
      421 cgagcctttc cggcacggcg gtgggctacg gggcaagcgc ctccgatccc ggcggctcgg
      481 tggcgaaggt cgagttctat ctcgcaccgg gcacgcagac cgccggcccg ctcgccccct
      541 tcgcgacgaa aacgtcggcg ccctacgccg gcacgctcga cacgagcaag gtggcgaatg
      601 gctcgtacac gctgatggcc gtggccaccg acaacctggg cgcggccgcc actacgcagc
      661 gccccgtcac cgtcagcaac gtcgtcgcgc caccggcccc gacccctgtc gcgaccgcgg
      721 acatcctcac ccgcgcaagc gccgacatgc cgttcgccca gcaaagcggc tacacggccc
      781 aggtcatcaa tacctatacc tcggccagcg cgatcccgga gtcgggcatc aggggctcgg
      841 cgcttccgga cggcgacacg atgcgtttcg gcaaggtggc cgacccgacg agctcggcac
      901 tcaaggccct cgccttccag gtgacgagca ccgacccgac cacttcgggc ggcaagcgca
      961 cggagctttc gatcgcgccc aatatccagc acgacaaggt gtactggatc gccttcagcg
     1021 cctacgtcca cgactggggc acgctcggcg cgaacgacct ggcgctgttc ggcacgcaac
     1081 tgcaccaggg caacaaccag ctcaaggtgg gcggcccggc cttcggcctc tatacgaagc
     1141 agaacggcag gtccttcagg gtgcgagggc gctatagcga ggccgcgact cccagcgaaa
     1201 gcaacgccta caacgtggag ttcaccgact acccgatccc cttcggccgc tggaccgact
     1261 tcgtgctcaa gttcaagcaa aacaccagcg gcaacggctt gctgcaggtg tggatggacg
     1321 gcgttcagat cgccgactac cacggcagcc tgggctacaa caccggctac aacgactaca
     1381 tcaagttcgg gtactacaac tggagtctca cgacgatgag ctcgaccccg cgcaaggtgc
     1441 tgctgcgctc gccgacgatc gtcgccgacc cgaccgggag caagtaccag cccgagcagt
     1501 tgagagcgct ggtgagcgcg gacgcagcgg cacagtgagg gcaccggcca tgcttaaacc
     1561 tcaccgcggc ttcacgctcc tcgagctcct ggtcgtgatc gtgatcatcg gaatgctcgc
     1621 cggctacgtc gccccgcgct acttctcgca cgtcgggaag tccgagaccc aggtggcgcg
     1681 tgcgcagatc gacgcgctgg aaaaggcgct cgacacctac cgcctggaca accgccgcta
     1741 cccgagcgcc gaggaagggc tcgcggcgat cgggccgtac ctgaagaagg cggtgccgaa
     1801 cgatccctgg ggccggccgt acgtctaccg cacccccggc cagcgcggcg agtacgaggt
     1861 cttctcctac ggccgcgacg gcaaggccgg cggcacgggc gaagacgccg acatcggtac
     1921 ctcgtagcaa tgcaattcaa cgtcctggca gtcgattcgc gccagcaggt cgtggcgctc
     1981 gacctcgaag cggcgagcga agcgctcgcc gccgaccagg cgcgcgcgcg cggcctgagc
     2041 gtcgtttccc tggagaggaa gggctttgcc ctgcccaagc gcaaggcccg cttcccggcg
     2101 acgctgttct cgatggagct gctgtcgctg ctgcaagccg ggctcaacct ggtggaagcg
     2161 ctgcaaacgc tctcagaaaa agagagcggc ggcgagcgca cggaagtgct ctcggcgatc
     2221 ctcgccgcca tccaccgcgg cgagccgttc tcgcaggcgg tcgcggcgct gccgcggcat
     2281 ttctccccgc tctacgtcgc caccatcaag gcggccgagc gcaccggcaa cgtgcccgag
     2341 gcgctcgggc gctacatcgc ctaccaggaa gagctcgacc gcgtgcgcaa gaaaatcgtc
     2401 tcggcgagca tctacccggc gatcctggtg gtcgtcggcg gcctggtgct cgcgttcctc
     2461 atgttctacg tcgtgccgcg cttcgcccgg gtctacgagg acatggccgg cacgctgccg
     2521 ctgttctcga agctgctgct cgctttcggc ggcttcgtcg gcaacaacgt cttcgttctc
     2581 gtgggcgcgt tctgcggcct ggtcgcgggc gcggtctggg cctggtcgcg ccccgaagtg
     2641 cgctcctggc tcaacaccca ggtctggcgc gtgccggcgc tcggcaagcg catgaaggtg
     2701 taccagctcg cgcggctcta ccgcaccacc gcgatgctgc tgcgcgccgg gattccggcg
     2761 gtgcgcgccc tcgacatggt gcgcgacctg cttgccgcgc atctgcggcc tcagttgatg
     2821 aaagcgaaga aatccatcga gcagggcgtc gccatgtccg ccgcgctcgg cgcggtgggc
     2881 ctcgccaccc cggtggcggc gcgcatgatg gtcgtcggcg agcgcagcgg cgacatgggc
     2941 gagatgctgt cgcagatcgc gcgcttccac gatgatgaaa tagcgcgcac cgtcgagtgg
     3001 ttcaccaagg ccttcgagcc gatcctgatg gcggtgctcg gcctcgcgat cggcggcgtc
     3061 gtggtgctca tgtacatgcc gatcttcgag ctcgcgggga gcatccgatg aagatggaaa
     3121 ccaccgtggt tcacaccctt gcggagctgt cgtcgcggct gcgcatgcct tgcgcttcga
     3181 tcgaggaaat gcgcgaaagc cgggccgcgt tcgacctcgt cccctacgcc gaagcttcgc
     3241 gccgcgcctg cgtcgcgctg cgcgccgccg acggcgcagt gtcgatcgtg ctcgccgagc
     3301 ccttcgacct cgacacccag gactggctcg aggagcgtct cggggtgccc ttccactatc
     3361 gcctcgccag ccgcgcggat gtcgaggcct atctcgccca gcaggaaacc gggctgcggg
     3421 cgatggacgg ggtctcgaaa gaccttcggg ccgacacgcg gagcgatggc gctcacgaaa
     3481 tatcattcga agcggcggcg gacgccgaca gcacggtggt gcgcctggtg agctcgacgc
     3541 tgtacgacgc gctgaaggcc ggcgcgagcg acgtgcacct cgagtcgacc gcgagcggcc
     3601 tctccatcaa gtaccgggtc gacggcgtgc tcaccagcgc ccagaccatg ccggggaccg
     3661 agctcgccga gcaggtgatc tcgcgcatca aggtgatgtc ggagctcgac atcgccgagc
     3721 gccgcgtgcc gcaggacggg cgcttcaagg cgcgccatcg aaacggagcc gacgcgggcc
     3781 gcgacatcga ctttcgcgtc tcggtgatgc cgagcgtgtt cggcgaggac gcagtgctgc
     3841 ggattctgga ccgccgtgct cttaccgatc agctgcaggg tttgtccctt gaagtgcttg
     3901 gcttcgaacc gggactgcgc gcgcgcctct ccgatctttc aaaagaaccc tacgggatgc
     3961 tgctcgtcac ggggccgacc gggagcggca agaccaccac gctgtatgcg gtgatctcgg
     4021 aggcgaacaa gggcctcgac aagatcgtca ccatcgagga cccggtcgag taccagcttc
     4081 ccggggtgct gcagatcccg gtgaacgagc agaagggcct caccttcgcg cgcgggctgc
     4141 gctcgatcct gcgccacgat cccgaccgca tcatggtcag cgagatccgc gacccgaaga
     4201 ccgccgagat cgcgatgcag tcggccctca ccggacacct ggtctacacc acggtgcacg
     4261 cgaacaacgc gatcgacgtg ctcgggcgct tccagcacat gggcgtcgac acctacaacc
     4321 tggtttccgc cctgaacggg gtactggcgc agcgcctcgt acgggttttg tgcaagacct
     4381 gccgttcgcg tggatgtgct gactgcaggg gaaccggttt caaggggcgc aaggcgatcg
     4441 gcgagctcgt ggtgctgaac gacgagctgc gtgagctgat cgtggcgcgc gccccggcaa
     4501 ggaaactcaa ggacgcggtg cgcgccgcgg gcaccgtcgc gctgcgcgag gcggcgatgg
     4561 cgctggtgca ggccggcgaa accactctcg aggagatcaa ccgtgtcact ttcgtggctt
     4621 aagaggaaac atctgcgaat cggccttggc gcgaagcgcg tcatggtctc cggcgccaag
     4681 gcagtcgagc tgccggccgc cgacgactgg cgcgcggcgc tcgaggcgct gcccgagatc
     4741 ctgaagtcgc acaagagccg cgaggcgagc gtggtgctcg ccgaccagtt cgtgcgctac
     4801 gcgctgctgc cctggaacga ggcggtgaag agcccggcgc agtggctcgc gctcgcgcgc
     4861 catcgcttcg gtgcgctgca cggcaccgcc gccgccgagt ggaacgtcaa ggtcaccgac
     4921 accgcgccgc gcggcccgcg cctcgcgtgc gcggtggatg gcggcctgat cgaagggctg
     4981 gcttgcgcct tcgtcggcgc gagcgtcacg ctcgtctccg tgcagccgtt cctcgtcgcg
     5041 gcgttcaacc ggatccggca gatggtcggc aacgcctcgt gctggatcgt ggtggaggag
     5101 cccggccgcc tcacgctcgc gctcatccag cgcggcgcgt gggtcgcgat ccgcagccgc
     5161 cgcagcgacg agcgctggcg cgtcgcgctg cccgagatcc tggagcgcga gggcgcgttc
     5221 ctcgggctca acgaggcctg cacccgggtg atcgtctgcg cccagggcga attcgatccg
     5281 cagatgcacg acgcgtggcg cgcccatgca atgagttatc gcgaactggc cctggcatgg
     5341 gaatgatgaa gaaaatccag attgatttcg ggagcgcggg ccgcggctcg ccgtgggtcg
     5401 gccgcgccct gctggcgatc gccgccgtgg tctgcgtcga cgccgcgttc acctatcaag
     5461 gtcttcacca gtccgtgaag gaaaacggcg cgcggctggc gaagcgctct tcgacgggcg
     5521 ccgtcgccaa ggtctccccg caggaggtcg cggcggtgcg cgagacggtg gagcgcatcg
     5581 cgctgccctg ggaccagctc ttctcggcgc tcgagtccgc ggcgagcgac aaggtggcgc
     5641 tcgccgccat cgagcccgac acggccaagg gcacggtgac gatctcgggc gagagcaagg
     5701 actatctcgc ggcgctcagc tacgtgctca acctcgggcg cagcgacagc ctcgaccacg
     5761 tgcagctcgt gcgccacgag ccgaaggcga acgacccgaa cggcgcggtg agcttcgcgg
     5821 tgtcggctgt ctggagcgca aaatgaagtt ttcactaaaa cccgtccagg agcgtatcgg
     5881 cgtcaccggc ctcgcggcca tcgcgctcct cgccgcggcg ctcgcgttct cgaatctcgt
     5941 ggtcaagccc ctgaaggaga agagcgcgct cctgcaggac cgcgtctcgc gccaggcgcc
     6001 cgacggcgcc cggccctccg ccgacaaggt ggcggcggtg taccagttcc tcgagaagga
     6061 ggagcaggcc accgactggc tggcgaagct gcacggcatc ggcgccgcca ccggcgtgca
     6121 gctcaagtcg gcgagctacc gcacccagaa gaccgacgcg cgcatcgtgc gctacgagat
     6181 ggtgctgccg gttgcgggca gctatgcgca gatccgcgaa ttcctccagc gctcgctcgc
     6241 cgaggttccg gtgctttccg tggaccagct caacctcaag cgggacggga agaatgccaa
     6301 cgtcatccag gcggaaatgc gcctcaccct ccacatggtg aagtcatgaa aatggacaaa
     6361 agacatattg ttctcgggat cgtagcggtg accctggttg cgggcgtcgt cgcaggccgc
     6421 gagcgccccg cggtcgagct cgcgcagccg cgcgcgccgc aggcagccgc ggcgaagtac
     6481 gacgacatcg atctcgacaa gctgcgccgc gccgaggtgc gagcgccgag cacggacccg
     6541 ttcgcggcca tgaaaggccc cgagatccgc gccgctgcgg ccgcggccgc gcccgaggcg
     6601 cccgcaaggc cgaccgcgcc accgctgccg ttccagtaca tcggcaagtg gagtcagggc
     6661 gacaagggcg aagtgctgct ccagcgcggc gaggagctgc tgcccatcgc gccgggcgcg
     6721 aagctcggcg actaccgcgt cgaccagatc accgaagcgc gcgtcagctt cacctacctg
     6781 cctctcaaca tgaagcagac cctcgacctg ctggggccgg aagcccggca atgaagggct
     6841 tcacgctcat cgagctcctg atcgtggtgg cgatcgtggc gctgctcgcc tcgatcgcgg
     6901 cgccgctcgc cgagctgggc taccagcgcg gcaaggagca ggagctgcgc tacgcgctgc
     6961 gcgagattcg cgaggcgatc gacgcctaca agcgggcctc cgacgaaggc aagatcgagc
     7021 gcaaggcgga cgcctcgggc tacccgccgt ccctcgcgac gctggtcgag ggcgtggagg
     7081 agaagaccaa cccggagaag accaagatct attttctgcg gcgcattccc aaggatccgc
     7141 tcggcggcga ggcgtggggc ctgcgcagct acgccagccc cgctgacgat ccgcgggacg
     7201 gcaaggacgt ctacgacgtg tattcacgca gcgagggtgt cgggctgaac aagctgccct
     7261 acagagagtg gtaggaacga gaatgacact gaagaagaga ctcggtttta cgctggtcga
     7321 gctgatggtg gtcctcaccg tcatcgccct tctcctgtcg gtcgtggtgc ccgactacgt
     7381 cgggcgcatg aagcgcgcgg aggagtcggc gctgcaggag aacctcgcgg tgatgcgcga
     7441 tgcgctcgac aagcactacg ccgacgccgg caagtatccc gccacgatcg aggagctggt
     7501 ttcgaagcgc tatctgcgct cgatcccgag ggacccgttc acccagaaag cggacacctg
     7561 ggtcgcggtg ccgcccgccg atcccaagag cggcggcgtg ttcgacatcc acagcgccgc
     7621 caagggctat gagcgctggt aggaccgcgg ttttcttcgc ccccctgaca agggggggaa
     7681 gcgagcgatt tttgcgagcg gggggtttgc ttttcatgaa aaccaacccc ccgctcgcct
     7741 ccggctcacg acccccttat cagggggtca aaaaaatcca gaaggggttt acctacgtgg
     7801 gattcctcct ggcagtcgcg atcatgggag ccgggctggt ggcgtattcc gagctcgcct
     7861 cgcacgccgc gcagcgggaa aaggaggcgg agttgctgtt ccgcgggaac cagtaccgcg
     7921 cagccatcgg ctcgtactac aaaaaagagc agcgctttcc gaagtccctg gcggacctgc
     7981 tggaggacaa gcgctatccg atgccggtgc gccacctgcg caagctctat cccgacccgg
     8041 tggccggaac cccggcctgg gggctgatcg aggcgcccga gggcggcatc atgggggtgt
     8101 acagcctgtc cgaagaagcg ccgatcaaga tcggcaactt cgccgcgcgc gatgccgcgc
     8161 tggccgaagc gccgcgcacg gcgcagtccg gcgagccgcg ctacgccgac tggaagttct
     8221 tctaccgccc tagtccgccc ggaccaaaag ccgccggttg agccgccgca tcgccggcgc
     8281 ttcaatcggg tgatgagcga aggatcgatt gcggtcgggg cgcgcgcctc gcccctgcgc
     8341 tggctgttcg gcgagctccc ctgcgcacga cccgcgggcc gcctgctcga cgtcgacgcg
     8401 gcgcgcgggc tggcgatcat cctggtcgtg gtcggccacg tcggggcgcg cgtcgccacg
     8461 ctcgaggacg ccggatggta tgcggacctg aaggcggcga tctatctctt tcacatgccg
     8521 ctgttcatgg cgctcaccgg gattaccttc gcgctgtcgc tgccggcatt caccgcctgg
     8581 agcgaggccg cgcgtttttc cttcaagcgc gtggagcggc tgctcgttcc ctacgtggtc
     8641 ttcggcctgg tcgtgatcct cggcaagctg gccgcctcgc gcgtgatgca cgtcgacaga
     8701 gcgccgcagg atctcgctgg cgagctctac ctgatgctgg tcatgccggg cagcagcgtc
     8761 accgtcttta tctggttcat ctacgtggtg tcgatctatc tgctgctcgt tccggcattg
     8821 ttccggctca tcggccggcg ccccatgctt ctgttcatcg cctcgctggt gctgcagctc
     8881 ggcgagtggc cgcagctttt catgctggac cgcgttgtcg agtacctgcc cttcttcgcc
     8941 ggcgggatgc tgctctggat gcaccgcgca gcctggttgc gcatcatgcc ggcggtgcta
     9001 tggccggcca ctatcgtctt cgtcgcgctg ctggtgctgt cgctgtggct caagctaccc
     9061 aaatggctgg tgggcgcggt gtcggttccg gcagtcctgg cgtggatgca cctgctggcg
     9121 gcgcagccgc agggatggct cgcggcgctc gggcgtgcga gcctcgccat ctacctgatg
     9181 aacaccatag ccatcgggct cgtaaaaggc gtgatgctga agttcctgcc ctggcacggc
     9241 gtcaactttc tgctgtattt tccgctgctt acgcttgccg ggatcgcgct tccgctgctc
     9301 gtcgcgagct tcgtcaagcg ccacgcgccg cgcgcaggcc gctatctggg aacggcatag
     9361 tccgtccgga ctatcccggc gcattgcaaa gtcgagcgcc aagtcacaga ttagggccgg
     9421 tgtagagaac gctctacaca cgatcggggt tctggccgac acactgttaa cagtccgcag
     9481 tcacggggcc aaacaacgtg tagtcaactg cgatcgccgc ttgagaagca cagcagcacg
     9541 gggggcgcgg ctagcggccg cgccctcttt atttcagggc gaggcggagc ccccggcgac
     9601 cgcggtttcc gcgggcgaat tcatccttcc ccagcggtcg tgccagttgc cgtcgaagca
     9661 gtgcatgagc tcgtggccga gggtggcgaa gcgctgcagg tcgcgctcgt tgcgcggcgc
     9721 gggcgcgtag atcgagcaca cccgcggcgc gccctcggcg gccggctcgg agcgatgcga
     9781 gcagccgagg atgttgaaga agttcttgcg gccggagagc gactcgcaga cggcgtgcgg
     9841 ctcctcgact cggttccaca ccaccgcgac gccgccgtct ttggagtcac cgggcgaagt
     9901 cgcgcacgcc gacagcaaaa tgaatactga tgaaagaaaa attcgcgtca cgcgatcagc
     9961 atgcgcccga cgtcgaggcc ctcggcgtac ttcacgccga ccatgcggcc gttcagccgg
    10021 gcgatgtcgc ggaacatctc ggcgtcgagg ccgcgcgtgc cgaactggct ggcgtgcgcc
    10081 gcgatcgcct ccatcttg
//
LOCUS       DATBSZ010000237         3353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2435917, whole genome shotgun sequence.
ACCESSION   DATBSZ010000237 DATBSZ010000000
VERSION     DATBSZ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2435917"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VIG70_15060"
     CDS             <1..450
                     /locus_tag="VIG70_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY8251918.1"
                     /translation="PEATAKALRGGWLHTGDVGSLDAEGFLTIKDRSKDMIISGGANI
                     YPREIEEVLLRHPAVAECSVVGRPHPEWGEEVVAFIVKKSALRIEELDSLCLDNIARF
                     KRPRDYRFVDALPKNNYGKVLKTELRKLLSAAGGPAAHPPAPSSDRR"
     gene            complement(392..892)
                     /locus_tag="VIG70_15065"
     CDS             complement(392..892)
                     /locus_tag="VIG70_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251919.1"
                     /translation="MRAAVFLLLAGCASIDGRGLVAGQSTVADVEAVMGAAADKRPGA
                     GGETVYYYPRLPWGYATYAARIAPDGKLVALEQRLTPENTEKLKPGVTRASEVCDLLG
                     PPYEPMKQALSGNEIWTYPMRTPGYPTPRWFLVHVSPEGVVRETHIIDDPNWVRGDAP
                     RGPRLH"
     gene            complement(912..1250)
                     /gene="fdxA"
                     /locus_tag="VIG70_15070"
     CDS             complement(912..1250)
                     /gene="fdxA"
                     /locus_tag="VIG70_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008528408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEY8251920.1"
                     /translation="MTYVVTEDCIKCKHMDCVEVCPVDCFYEGENMLVIHPDECIDCG
                     VCEPECPPKAILPDSDPKAEKWLKLNADFSVKWPNITRKGTPPPDADAWKEVPGKFEK
                     YFTPEPGKGE"
     gene            1379..3328
                     /locus_tag="VIG70_15075"
     CDS             1379..3328
                     /locus_tag="VIG70_15075"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8251921.1"
                     /translation="MSRHTFLAPFLIISGAFLLILTAGAEIVAAFAVWLAGEGDLPGE
                     LILHALVAGAALMLAAAVIMTRRSVQRTAAAEAALRESEERLRLVANNVPALISYVDR
                     ERRYRFSNRTYDDWFGIPHERMQGRSVAEVFGEETYERMQPDIERCMAGDSVEFELTT
                     ASGGKARMLQVACVPHLGREGEVLGFYMLANDVTALKRAQEDLRVAAARLQNDAQRLE
                     FLAHHDTLTGLPNRAMFAERAREAVAHARRHDKTAAFLFLDMDNFKQVNDSLGHEVGD
                     GLLKVMAARLKASVRGDDFVARIGGDEFCVLLQDISEPREAAAVAQKLLYELGKPYRV
                     GGHELASGASIGIACVPQDGDEVDTLLRLADHAMYRAKELGRNGYQFFSGILNDDAAA
                     AVELAEDLRAGVGRGELFLVYQPRVDIATRKAVGAEALLRWRHPRHGVLAPEAFLPLA
                     DDTGLLVEVGGWILREACIQGRRWIEAGIHPFSMVVNMSARQLRSEGLAGHVRDALKA
                     SGLPAGSLLIEVPETSVRQISEPIEQALAEITASGARLSVDDFGTGYASLPMLQRLRA
                     SAVCIDRSLIAGVPHDSERAGLARALIVLARGLEFEVVAKGVETQAQRDFLIEAGCRI
                     CQGELFAPARAAEEVEPFLRSRRAA"
BASE COUNT          514 a         1172 c         1148 g          519 t
ORIGIN      
        1 cccgaggcga cggcgaaggc gctgcgcggc ggctggctcc acaccggcga cgtcgggagc
       61 ctggacgccg agggcttcct caccatcaag gaccgctcga aggacatgat catctcgggc
      121 ggcgccaaca tctacccgcg cgagatcgag gaggtgctgc tgcgccaccc ggcggtcgcc
      181 gagtgctcgg tcgtcggccg tccgcacccg gaatggggcg aggaagtcgt cgctttcatc
      241 gtcaagaaat cagcgttgag aatcgaagag ctcgattccc tgtgcctgga caacatcgcg
      301 cgcttcaagc ggccgcggga ctaccgcttc gtggacgcgc tgccgaagaa caactacggc
      361 aaggtgctga aaaccgagct gaggaagctc ctcagtgcag ccgggggccc cgcggcgcat
      421 ccccccgcac ccagttcgga tcgtcgatga tgtgggtctc gcgcaccacg ccctcggggg
      481 agacgtggac caggaaccat ctcggcgtgg ggtatccagg ggtgcgcatc gggtaggtcc
      541 agatttcgtt gcccgaaagc gcctgcttca tcggctcgta cggcggaccg agaaggtcgc
      601 acacctcgct ggcgcgggtc acgccgggct tgagcttctc ggtgttctcg ggggtgaggc
      661 gctgctcgag cgcgaccagc ttgccgtcgg gcgcgatgcg cgcggcgtag gtggcgtaac
      721 cccaaggtaa tcgcgggtag tagtacaccg tctcgccgcc ggcgcccggg cgcttgtcgg
      781 cggccgcgcc catgaccgcc tcgacgtcgg cgacggtcga ctggccggcc acgagccccc
      841 tgccgtcgat gctggcgcag cccgcgagca gcaggaagac cgccgctcgc atcaacgccc
      901 gcatgccttt cctattcgcc cttgccgggc tcgggggtga agtacttctc gaacttgccc
      961 ggcacttctt tccacgcgtc ggcgtcgggc ggcggcgtcc ccttgcgcgt gatgttcggc
     1021 catttgaccg agaagtcggc gttcagcttg agccacttct ccgccttggg atcggagtcg
     1081 ggcaggatcg ccttaggcgg gcactcgggc tcgcacacgc cgcagtcgat gcactcgtcg
     1141 ggatggatga cgagcatgtt ctcgccctcg tagaagcagt cgaccgggca gacctcgacg
     1201 cagtccatgt gcttgcactt gatgcagtct tcagtgacga cataagtcat agggcgcgca
     1261 ttataaagcg ccgagaaccg gcgtagcgaa gcgtcgactt atagctcatt gaattaccta
     1321 cagtgcgccg gggcataatg ccgttgcccc gccccgccgg gagggcgatc attgcgcgat
     1381 gtcccggcac accttcctcg cgccgttcct tatcatttcg ggcgcgttcc tgctaatcct
     1441 taccgccggc gcggagattg tcgccgcgtt cgcggtctgg ctggccggag agggcgatct
     1501 gccgggagag ctcatcctgc acgcactggt cgcgggcgcg gcgctgatgc tcgcggcggc
     1561 ggtgatcatg acgcgccgct cggtgcagcg cacggccgcg gccgaggcgg cgctgcgcga
     1621 aagcgaggag cggctgcgcc tggtggcgaa caacgtcccc gcgctcatct cctacgtcga
     1681 ccgcgagcgg cgctaccgct tcagcaaccg cacctatgac gactggttcg gcatccccca
     1741 cgagcgcatg cagggccgca gcgtcgcgga ggtgttcggc gaggaaacct acgagcgcat
     1801 gcagccggac atcgagcgct gcatggcagg cgacagcgtg gaattcgagc tcaccaccgc
     1861 gagcggcggc aaagcacgca tgctgcaggt cgcgtgcgtg ccgcacctcg gccgggaggg
     1921 agaggtcctc gggttctaca tgctcgcgaa cgacgtgacg gcgctcaagc gcgcgcagga
     1981 ggacctgcgc gtcgccgccg cgcggctgca gaacgacgcg cagcgcctcg agttcctcgc
     2041 ccaccacgac acgctcaccg ggcttcccaa ccgcgcgatg ttcgccgagc gcgcgcgcga
     2101 ggccgtggcg cacgcgcgcc ggcacgacaa gaccgcggcg ttcctgttcc tcgacatgga
     2161 caacttcaag caggtcaacg acagcctcgg ccacgaggtc ggcgacggcc tgctgaaggt
     2221 catggccgcg cgcctgaagg cgagcgtgcg cggcgacgac ttcgtcgcgc gcatcggcgg
     2281 cgacgagttc tgcgtgctcc tccaggacat ctccgagccg cgcgaggcgg cggcggtggc
     2341 gcagaagctg ctgtacgagc tcggcaagcc ctaccgcgtc ggcgggcacg agctcgcttc
     2401 cggcgcaagc atcggcatcg cgtgcgtgcc gcaggacggc gacgaggtgg acacgctgct
     2461 gcggctcgcc gaccatgcca tgtaccgcgc caaggagctc ggccgcaacg gctaccagtt
     2521 cttctccggg atcctgaacg acgacgccgc ggccgcggtc gagctggccg aggacctgcg
     2581 cgccggcgtc gggcgcgggg agctgttcct cgtgtaccag ccgcgcgtcg acatcgccac
     2641 gcgcaaggct gtcggcgccg aggcgctgct gcgctggcgc catccgcgcc acggcgtgct
     2701 cgcgccggag gccttccttc cgcttgccga cgataccggc ctccttgtcg aggtcggcgg
     2761 ctggatcctg cgcgaggcct gcatccaggg ccggcgctgg atcgaagccg gcattcatcc
     2821 gttctcgatg gtcgtcaaca tgagcgcgcg ccagctgcgc agcgagggcc tcgccggcca
     2881 tgtgcgcgat gcgctgaagg cgagcggctt gcccgcgggg tcgctcctca tcgaggtccc
     2941 cgagacttcg gtgcgccaga tctccgagcc catcgaacag gccctcgccg aaatcaccgc
     3001 cagcggcgcc cgcctcagcg tcgacgactt cggcaccggc tacgcgtcgc tgcccatgct
     3061 gcaacggctg cgcgcgagcg ccgtctgcat cgaccgcagc ctgatcgccg gcgtgcccca
     3121 cgacagcgag cgcgccggcc tcgcgcgcgc cctgatcgtt cttgcccgcg gcctggagtt
     3181 cgaggtcgtg gcgaagggcg tcgagaccca ggcgcagcgc gacttcctca tcgaggccgg
     3241 ctgccgcatc tgccagggcg agctgttcgc ccccgcgcgc gccgccgaag aagtcgagcc
     3301 cttcctgcgc tcgcgccgcg ccgcctagac accgccgcgc taagataacc ggc
//
LOCUS       DATBSZ010000238         5569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_864114, whole genome shotgun sequence.
ACCESSION   DATBSZ010000238 DATBSZ010000000
VERSION     DATBSZ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864114"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..684)
                     /locus_tag="VIG70_15080"
     CDS             complement(22..684)
                     /locus_tag="VIG70_15080"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY8251922.1"
                     /translation="MQKIFKVILVAAALLAAAPALAQSNRAGKGEIYLGPVFTDGESY
                     VFDGGTTARTDTGFGISLGYAYNFNSHWQAGIDFAWSETDYRANVQPGQGNTNFSGNI
                     NGTVETSTIRFTGTYHFLTSEFTPFVTGGLGWTYVDTNIPSGLPESFCWYYPWYGSYC
                     GTYVPTAATTKFSYNAGVGVRLDVGRGFFRGLVNQQWIQFSDGYGSQAWTQYRIDFGT
                     KF"
     gene            complement(687..944)
                     /locus_tag="VIG70_15085"
     CDS             complement(687..944)
                     /locus_tag="VIG70_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251923.1"
                     /translation="MPEQRRLIIHFVDGSKKLLEFPQQVADGDATMAARLKEALEARH
                     LVIEADGALVIIPFENIRYLQAYPAPKKLPGYAIKGATFKD"
     gene            complement(962..1324)
                     /locus_tag="VIG70_15090"
     CDS             complement(962..1324)
                     /locus_tag="VIG70_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251924.1"
                     /translation="MTVWAQTCLVCGEVRSIREMSASGLSQRPAPATPGSPSGLDTGP
                     VVGTVAQFHFGGGERSDGWSFGAAGTPDMQARLGETTYEITVAMDMGEKRTLQRRDGN
                     RFHVGQRVALRQGELEPM"
     gene            complement(1364..2212)
                     /locus_tag="VIG70_15095"
     CDS             complement(1364..2212)
                     /locus_tag="VIG70_15095"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8251925.1"
                     /translation="MLVRSFFLLVLLGAPFLAGAQTLDKIRKSGVITLGYIDGAAPFS
                     YTDGNNEPQGYSVELCRTVADGVAAQLKRQGLKVRWVKLTIQDRIEAVRAGKVDLECS
                     TTTWTLTRQQLVDFSLVTFVDGGSVLVKGDAELARLADFDGKRLAVISGTTTERALRA
                     GLARHTVKAQIATVKTRDEGLALLREGQVEGFASDRTSLIGLVVMSGTGRGTYKLLDQ
                     DFSIEQYALMLRRNDHDWRLAVNRVLARVYRTGEIEKIYGRWLGPLGPPSLLLSAAYF
                     IQTLSE"
     gene            complement(2215..3267)
                     /gene="ydiK"
                     /locus_tag="VIG70_15100"
     CDS             complement(2215..3267)
                     /gene="ydiK"
                     /locus_tag="VIG70_15100"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HEY8251926.1"
                     /translation="MIEKGQGDLARILLVVLFLGGLIAASFWILRPFLGAIIWAVMIV
                     VSTWPLMLAAQKRLGGRRWAAVTVMSLVLLLILVVPLSLAIGTIVANVDNIVDWAKRL
                     SEFKLGAPPSWLAGLPLVGERAVTLWQDVAAQGAEGVSARIAPYTRALAAWFVAEMGS
                     FGALFIQFLLTVIAAAVLYAQGEEAADWVLRFGARVGGEPGEGAVRLSAQAIRGVALG
                     VVVTAVVQSLIGGIGLAVAGVPFAAVLTAVMFLLAVAQIGAVPVLIVPVIWLYWTGEA
                     GWGTFLLVVTVVAGTLDNVVRPILIKKGANLPLLLIFLGVIGGLIAFGLIGIFVGPLV
                     LAVTYTLLSAWLYKKG"
     gene            complement(3287..3544)
                     /locus_tag="VIG70_15105"
     CDS             complement(3287..3544)
                     /locus_tag="VIG70_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251927.1"
                     /translation="MRLVIVLAALAGLLSACADMKTRVAESRQDRCERANWKDVGLRD
                     GIQGAAMQADRYKDICGELFKPAPYDEGLREGLARRPRPPA"
     gene            complement(3553..4968)
                     /locus_tag="VIG70_15110"
     CDS             complement(3553..4968)
                     /locus_tag="VIG70_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY8251928.1"
                     /translation="MRRSIVLVGTLLSACAVGPDYERPAVESPPEYRIDYPKAAEVAN
                     TKWWEQFGDPALNQLIEEALLGNLDVKAAAANVDRFLGALRTTRSQFFPQFGYGADAS
                     YNRASRVGQPPLGSGDDNTYSLYQGSLSADWQIDLFGRVRRQSEAVQAQVYASEQGRR
                     GVILSIVSTAAVAYVSLRALDRQLEIARGTAQNYAETRKLFELRLRGGVISDVEMAQI
                     ESQYELALAAIPAFEFSIAQQENLISTLLGRHPGPIPRGRTIDELAPPAIPAGLPSEL
                     LERRPDILQAEQNLVTANAGIGAAKALYFPSFSLTGAIGTASTDFSNFLTGPARTAFI
                     AASLVGPIFTFGNIEGQVRSAEAAREQALYFYRQTILNALRETNDALVGSQKTREQSE
                     AQTRRVRALRNYARLSRVRFDNGAASYVEVLVAENDLFSAELNLVTNQADRLTQVINV
                     YKAMGGGWVDLSDLLASPEKK"
     gene            complement(4969..>5569)
                     /locus_tag="VIG70_15115"
     CDS             complement(4969..>5569)
                     /locus_tag="VIG70_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8251929.1"
                     /translation="GQALQEKESGSTSSSAFIFGLIVVFLLLAAQFEKWTLPVAVVLT
                     VPFAIFGALVFTWALGLENDVYFQVGMVTLVGLSAKNAILICEVAIEKVHHGMGAREA
                     AIEAAGMRLRAIVMTSFAFILGCVPLAIATGPGANSLRAIGTGVIGGMLASTIIAIFF
                     VPLFFWMLESMSERFASKKEPAPAGGAAPKAAHAPHKDE"
BASE COUNT          939 a         1923 c         1829 g          878 t
ORIGIN      
        1 caataaccaa tttccgggtc atcagaactt ggtgccgaag tcgatgcggt actgcgtcca
       61 ggcctgcgag ccgtagccgt cgctgaattg gatccactgc tggttcacca gcccgcggaa
      121 gaagcccctg ccgacgtcca ggcgcacgcc gacgccggcg ttgtagctga acttcgtcgt
      181 cgcggccgtg ggcacgtagg tgccgcagta cgagccatac cacgggtaat accagcagaa
      241 cgactccggc aggcccgacg ggatgttggt gtcgacatag gtccagccca ggccgccggt
      301 gacgaagggg gtgaactcgc tggtcaagaa gtgataggtg ccggtgaagc ggatcgtcga
      361 ggtctcgacg gtgccgttga tgttgcccga gaagttggtg ttcccctgtc cgggctgcac
      421 gttggcgcgg taatcggtct cgctccacgc aaagtcgatt cctgcctgcc agtgcgagtt
      481 gaagttgtag gcatagccca ggctgatgcc gaagccggta tcggtgcgcg cggtcgtgcc
      541 gccgtcgaag acgtagcttt cgccgtcggt gaacaccggc ccgaggtaga tctctccctt
      601 gccggcgcgg ttggactgcg cgagggccgg cgcggcggcg agcagcgcgg cggcaaccag
      661 gatcactttg aatatctttt gcatgattag tccttaaagg tcgcaccctt gatggcgtac
      721 cccggcagct ttttcggcgc cggatacgcc tggaggtagc gaatgttttc gaatggaatg
      781 atcaccagcg cgccgtcggc ctcgatcacg aggtggcgcg cttccagcgc ctctttcagg
      841 cgtgcggcca tggtagcgtc gccatcggca acctgctgcg gaaactcgag cagcttcttc
      901 gagccatcca cgaaatggat gatcaagcgg cgttgttccg gcattcagtc tcccttgggg
      961 ttcacatcgg ttccagctcg ccctggcgca gcgcgacgcg ctggccgacg tggaagcggt
     1021 tgccgtcgcg gcgctggagc gtgcgcttct cgcccatgtc cattgccacg gtgatctcgt
     1081 aggtggtctc gccgaggcgc gcctgcatgt cgggcgtgcc cgccgccccg aagctccagc
     1141 cgtcggagcg ttcgccaccg ccgaaatgga actgcgccac cgtgccgacc acggggccgg
     1201 tatcgaggcc cgacggcgag ccgggcgtgg ccggcgcggg cctctgcgag agacccgacg
     1261 cgctcatctc gcggatcgag cgcacctcgc cgcacaccag gcaggtctgc gcccagacgg
     1321 tcaaagccga aaaaccgaaa aaggccgcaa gagcgcgcat ttgctactcg gacagggtct
     1381 ggatgaaata cgccgccgaa agcagcaggc tcggcgggcc gagcggcccg agccagcggc
     1441 cgtagatctt ctcgatctcg ccggtgcggt agacacgcgc gagcacgcgg ttcaccgcca
     1501 ggcgccagtc gtggtcgtta cgccgcaaca tcagcgcgta ttgctcgatg gagaagtcct
     1561 ggtccagcag cttgtaagtc ccccgcccgg ttccgctcat caccaccagg ccgatcagcg
     1621 aagtgcgatc ggaggcgaag ccctcgacct gcccttcgcg caagagcgcg aggccttcgt
     1681 cgcgcgtctt cacggtggcg atttgcgcct tgaccgtgtg gcgcgcgagc cccgcgcgca
     1741 gcgcccgctc ggtggtcgtg ccgctgatca ccgccaggcg cttgccgtcg aagtccgcga
     1801 gccgggcgag ttcggcgtcc cccttgacca gcacgctgcc gccgtcgacg aaggtcacca
     1861 ggctgaagtc gacgagctgc tggcgcgtga gcgtccaggt ggtggtgctg cactcgaggt
     1921 cgactttccc ggcgcgcacc gcctcgatgc ggtcctggat ggtgagcttg acccagcgca
     1981 ccttgagccc ctggcgcttg agctgcgccg cgacgccgtc ggccaccgtg cggcacagct
     2041 cgaccgagta gccctgcggc tcgttgttgc cgtcggtgta ggagaacggc gccgcgccgt
     2101 cgatgtagcc gagcgtgatc acgccgctct tgcggatctt gtccagggtc tgcgcccccg
     2161 cgaggaacgg cgctccgagc aggaccagaa ggaaaaaact gcgcaccagc atcgtcatcc
     2221 tttcttgtag agccacgcgc tgagcaaagt gtatgtcacc gccaacacca gcggcccgac
     2281 gaagattccg atcagcccga acgcgatcag gccgccgatc acgccgagga agatgaggag
     2341 cagcggcagg ttcgcgccct tcttgatcag gatcgggcgc accacgttgt cgagcgtgcc
     2401 cgcaaccacg gtgaccacga gcaggaacgt gccccagccc gcctcgccgg tccagtacag
     2461 ccagatcacc ggcacgatca gcaccggcac cgcgccgatc tgggccaccg ccaggaggaa
     2521 cattaccgcc gtcaggaccg ccgcgaacgg cacgcccgcg accgcaaggc cgataccgcc
     2581 gatcagcgac tgcaccaccg ccgtgaccac cacgccgagc gccacgccgc ggatcgcctg
     2641 cgcagagagg cgcaccgcgc cttcgcccgg ctcgccgccg acgcgcgcgc cgaagcgcag
     2701 cacccagtcg gcagcttctt ccccctgcgc gtacaggacc gcagcggcga tcaccgtgag
     2761 caggaactgg atgaacagtg cgccgaagct gcccatctcg gccacgaacc acgccgccag
     2821 ggcccgcgtg tagggcgcga tcctggccga caccccctcg gcgccctgcg ccgccacgtc
     2881 ctgccacagc gtgaccgcgc gttcgccgac cagcggcagg cctgcgagcc acgagggcgg
     2941 cgcccccagc ttgaattcgc tcaggcgctt cgcccagtcc acgatgttgt cgacgttggc
     3001 gacgatcgtc ccaatcgcga gcgacagcgg cacgaccagg atcagcaaca ggacgagcga
     3061 catcaccgtg accgcagccc agcgccggcc gcccagccgc ttctgcgccg cgagcatcag
     3121 cggccaggtc gagaccacga tcatcaccgc ccagatgatc gcgccgagga acggccgcag
     3181 gatccagaac gaagcggcga tcagcccgcc gaggaacagc acgacgagca ggatgcgcgc
     3241 gagatcgccc tggcccttct cgatcatatt cccttcggca gcacactcat gccggcggcc
     3301 tgggccgccg cgccaagccc tcgcgcaggc cctcgtcgta gggcgccggc ttgaatagct
     3361 caccgcagat gtctttgtag cgatcggcct gcatcgcggc gccctggatg ccgtcgcgca
     3421 ggccgacgtc cttccagttg gcgcgctcgc agcggtcctg gcgcgattcg gcaaccctcg
     3481 tcttcatgtc ggcgcacgcc gagaggagtc ctgcaagagc ggcaagcaca atcacgagtc
     3541 gcattcggcc tcctatttct tctcgggaga ggcgagcagg tcgctcaggt ccacccagcc
     3601 tccgcccatg gccttgtata cgttgatgac ctgcgtcagc cggtcggcct ggttggtgac
     3661 caggttcagc tcggcgctga agagatcgtt ctccgccacc agcacctcga cgtagctcgc
     3721 cgcgccgttg tcgaagcgca cgcgcgaaag gcgcgcgtag ttgcgcagcg cgcgcacgcg
     3781 ccgggtctgc gcctccgact gctcgcgcgt cttctgcgag ccgaccagcg cgtcgttggt
     3841 ctcgcgcagc gcgttaagga tggtctggcg gtagaagtac agcgcctgct cccgcgcggc
     3901 ttcggccgag cgcacctggc cttcgatatt gccgaaggta aagatcggcc ccaccaggct
     3961 ggcggcgata aaggccgttc gcgccggccc ggtaaggaaa ttgctgaagt cggtgctggc
     4021 cgtgccgatg gcgccggtga gcgagaacga cgggaagtaa agcgccttcg ccgcgccgat
     4081 gccggcgttg gcggtgacca ggttctgctc cgcttgcagg atgtcgggcc ggcgctcgag
     4141 cagctcggag ggcaggccgg cggggatcgc cggcggcgcg agctcgtcga tcgtcctgcc
     4201 gcgcgggatc ggcccggggt ggcgcccgag gagcgtcgag atcaggttct cctgctgggc
     4261 gatggagaac tcgaacgccg ggatcgcggc gagcgcgagc tcgtactgcg actcgatctg
     4321 cgccatctcg acgtccgaga tcacgccgcc gcgcaggcgc agctcgaaca gcttgcgcgt
     4381 ctcggcatag ttctgcgcgg tgccgcgcgc gatctcgagc tggcggtcga gcgcgcgcag
     4441 gctgacgtag gccaccgcgg cggtgctgac gatcgacagg atcacgccgc gcctgccctg
     4501 ctcgctcgcg tagacctgcg cctgcaccgc ctcgctctgg cgccgcacgc ggccgaagag
     4561 gtcgatctgc cagtccgcgc tcaaactccc ctggtagagc gaataggtgt tgtcgtcgcc
     4621 ggacccgagc ggcggctggc cgacgcggct cgcgcggttg tagctcgcgt ccgcgccgta
     4681 gccgaactgc gggaaaaact gcgagcgcgt ggtgcgcagc gcgccgagga accgatcgac
     4741 gttcgccgcg gccgccttca cgtcgaggtt gccgagcagc gcctcttcga tcagctggtt
     4801 gagcgccggg tcgccgaact gctcccacca cttggtgttc gcgacctcgg cggccttcgg
     4861 gtagtcgatg cggtactcgg gcggcgactc gaccgccggc cgctcgtagt ccgggccgac
     4921 cgcgcaagct gaaagcaatg tcccgacgag gacaatactc cgcctcattt attcatcctt
     4981 gtgaggtgca tgcgccgcct tgggcgcggc gccgccggcg ggcgccggtt ccttcttgct
     5041 cgcgaatctc tcgctcatcg attccagcat ccagaagaac agcggcacga agaagatggc
     5101 gatgatggtc gaagcgagca tgccgccgat cacgccggtg ccgatcgcgc gcaagctgtt
     5161 agccccgggc cctgtcgcga ttgcgagcgg cacgcagccc aaaatgaacg cgaacgaggt
     5221 catcacgatc gcgcgcagcc gcatcccggc cgcctcgatc gcggcctcgc gcgcccccat
     5281 gccgtggtgc accttctcga tcgcaacttc acaaataagg atggcgttct tcgcggagag
     5341 ccccaccagg gtcaccatac cgacctggaa atagacgtcg ttctccagcc ccagggccca
     5401 ggtgaacacg agcgcgccga agatcgcgaa cggcacggtc agcaccaccg ccaccggcag
     5461 ggtccacttc tcgaactgcg ccgcgagcag caggaacacc acgatcagcc cgaagatgaa
     5521 ggccgaggac gaggtgctgc ccgactcctt ttcctgcagc gcctggccg
//
LOCUS       DATBSZ010000239         6609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_318008, whole genome shotgun sequence.
ACCESSION   DATBSZ010000239 DATBSZ010000000
VERSION     DATBSZ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318008"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VIG70_15120"
     CDS             <1..957
                     /locus_tag="VIG70_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8251930.1"
                     /translation="QFSQAAKLTAAMRTEIGKALIGQPEVIDAVLCALLAGGHVLVEG
                     VPGLGKTLLVKALARTISGTFGRIQFTPDLMPADVTGHTLYDPKNQVFTTRRGPVFVN
                     LLLADEINRAPAKTQAALLEVMQEAQVTIEGEAHRLEPPFMVLATQNPIEQEGTYALP
                     EAQLDRFLLKVKIDYPSQADELALVRHVTEGKVGDRLEVEQVASLVKPATVTSLQAIT
                     ASLGADDAVLDYALRLVRATRAWAGVVAGAGPRGAISLVRAARAAALLQGRDFVTPDD
                     VKKMALPALRHRIMPSPELELEGRDTDAILKGLLEKIEAPRK"
     gene            954..2258
                     /locus_tag="VIG70_15125"
     CDS             954..2258
                     /locus_tag="VIG70_15125"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8251931.1"
                     /translation="MIPSARLLWAVLVIAAIAVAVSIYPEFQLPWTGFAAGFVLLALL
                     DAFAGLRLPAPALARNVPGSLALGVSSEVRLRIANAAGLRVRLDLHDHHPPSFEAEGL
                     PRRLTLERGQWTEVAYQVRPVARGETQFSAAEARLFSPIGLWQISRKIGAPSPVRVYP
                     NFRALAKYTLLATDNRLSQIGVLQVRKRGEGMEFHQLREYRQGDSQRAIDWKATSRTQ
                     RLIAREYEEEKDQRVMLVIDCGRRMASKDDELSHFDHALNAALLLAHVALRQGDAVGM
                     MTMAGINRFVEPRKSVGAVNAVLNRVYDIEPTLEVPDYNLAARDVMRRLRRRSLVIVL
                     TNLRDEDDETLLPALKLLRTRHLVVLASLREAIISRALSARVDSFDRAVTHAAAAEYL
                     AARERTFRRIGASGAVCLDVEPERLAISLVNRYLELKREGRL"
     gene            2289..2558
                     /locus_tag="VIG70_15130"
     CDS             2289..2558
                     /locus_tag="VIG70_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEY8251932.1"
                     /translation="MKPGIHPDYKAVIFLDTSSNHSFLTRSAIDTKGRETMKWEDGKE
                     YPVVKIEVSSESHPYYTGKQKIVDTAGRVEKFKRRYTKKTPEKKA"
     gene            2573..4198
                     /locus_tag="VIG70_15135"
     CDS             2573..4198
                     /locus_tag="VIG70_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251933.1"
                     /translation="MLPRLSLPPTPAALALIALAFALPGLAGHDLWKTHDAIGLGIVH
                     DMAESGAPLVPRIAGNPWLFDPPLYHWLALSFGKALQFILEFHSAARLASGTLVLAAF
                     FLIYLAARRWARPDDDRRVHGAAAMLVLLGSIGLIVHAHEALPELAALAALCGALAAL
                     PQAARRPLLAGALFGAAVGIAALSAMWISPAALLLAVIAAHVLCPQWRTRSAWVFVPV
                     SLLAALAVAASWPLALAWREPETFALWRHVAWQPIGDPVANLRYFLVTGSWFAWPAWP
                     LAVWAAWSLRRRWSEPRLFVPAVASVLLLLFAAYWGPAQDVHLIPLLAPLALLGSQGA
                     LVLRRGAAGALDWFGVLGFGFFGLLIWFCWFAMLTGVPARFANNFFKTAPGFSPEFKL
                     AYVAFALVLAAGWVVLIFYTTRSPMRSVLRWAAGIVLLWGTGAMLLMPWADYQKSYRS
                     VALQLRSKIAVGSGCIAQKALGVSQAAALDYHAGIRARPYDLVRPNACPLLLVQGNPL
                     HEFDGPGAGWVKLADVGRPGDRAERYRLYRLGK"
     gene            4195..5616
                     /gene="pgi"
                     /locus_tag="VIG70_15140"
     CDS             4195..5616
                     /gene="pgi"
                     /locus_tag="VIG70_15140"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY8251934.1"
                     /translation="MNTRSPTQCPAWAKLSQHAESWRAVHLRELFSADAARGRQFVAE
                     TPGLRYDYSRQRLGAITLRLLAHLAAERGFAEWREALLAGSEVNSTEKRAAWHTALRA
                     GDGAPREVKETLARMKELSGKLRAEKRFKRVINLGTGGSDLGPRLLADALSDNALDVR
                     FAANVDPRDLARALQGAEPASTLLIVASKTFTTQETMANALAAKEWGAKAFYAVTSNV
                     QAAEAFGAAEILPMWDWVGGRFSVWSAVGFAAACAIGFDSFQQFLQGGNDIDRHFAET
                     PLEKNVPALMALIGVWNANFLGSPTHAVLPYANALRLLPAFLQQLEMESNGKRVDREG
                     RVVDYATAPVLWGGEGTVSQHSFHQLLHQGTHAVPADFVDVGESRVLTANLRAQADAL
                     AYGTDDPKLPPHQQYPGNRPSSVLSLEKMDARNLGRLIALYEHKVFTQGVIWNINSFD
                     QWGVELGKKLAQKILTEGKNEER"
     gene            5616..5822
                     /locus_tag="VIG70_15145"
     CDS             5616..5822
                     /locus_tag="VIG70_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HEY8251935.1"
                     /translation="MDQETFNLSIRKFLKMVGVNSQREIEQAVQKSLQSGKLKGEETI
                     PVKVMLEIPALGVKVPFDGEIRLK"
     gene            5827..>6609
                     /locus_tag="VIG70_15150"
     CDS             5827..>6609
                     /locus_tag="VIG70_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY8251936.1"
                     /translation="MNKAREFDQSVVAELKALLGERVSTAAAVREHHGKDESYFPYAP
                     PDVVVFVRTTEEVRDVVNICRRHRVPMIPYGVGTSLEGHILAIAGGVSIDLSQMNQVL
                     AVHEQDLDAVVQAGVTRKQLNEFIKHTGLFFPVDPGADATLGGMAATRASGTNAVRYG
                     TMRENVLSLKVVLADGRIIQTSRRAKKSAAGYDLTRLFVGSEGTLGIITEVTVRLYPV
                     QEAMSAAVCAFDSVDGCTNTVIQTIQSGIPIARCDIVCEKTVD"
BASE COUNT         1032 a         2311 c         2223 g         1043 t
ORIGIN      
        1 caattcagcc aggccgccaa gctcaccgcg gcgatgcgca ccgagatcgg caaggcgctg
       61 atcggccagc ccgaggtcat cgacgcggtg ctgtgcgcgc tgctcgccgg cggccacgtg
      121 ctggtcgagg gcgtgcccgg cttggggaaa acgctcctgg taaaggcgct cgcgcgcacg
      181 atctccggca ccttcggccg catccagttc acgccggacc tcatgcccgc cgacgtcacc
      241 gggcatacgc tctacgatcc gaagaaccag gtgttcacga cgcgccgcgg gccggtcttc
      301 gtgaacctgc tgctcgccga cgagatcaac cgcgcgccgg cgaagacgca ggcggcgctg
      361 ctcgaggtga tgcaggaagc gcaggtcacg atcgagggcg aggcgcaccg gctcgagccg
      421 ccgttcatgg tgctcgcaac ccagaatccg atcgagcagg agggcaccta cgccctgccg
      481 gaagcgcagc tcgaccgctt cctgctgaaa gtgaagatcg actatccgtc gcaggccgac
      541 gagctcgccc tggtgcgcca tgtcaccgag ggcaaggtcg gcgaccggct ggaggtggag
      601 caggtagcaa gcctggtcaa gcccgccacc gtgacctcgc tgcaggcgat taccgcctcg
      661 ctcggcgccg acgacgcggt gctcgactac gcgctgcgcc tggtgcgcgc gacgcgcgcc
      721 tgggcggggg tggtcgcggg agccgggccg cgcggcgcca tttccctggt gcgcgcggcg
      781 cgcgccgcgg cgctcctgca gggccgcgac ttcgtgacgc cggatgatgt caagaaaatg
      841 gcgctgcccg cgctgcgcca tcgcatcatg ccctcgcccg agctcgagct cgagggacgc
      901 gacaccgacg cgatcctgaa aggtcttctc gagaaaatcg aagccccgcg caaatgattc
      961 cttccgcccg gctcctttgg gcggtgctgg tgatcgcggc gatcgcggtc gcggtctcga
     1021 tctacccgga gttccagctt ccctggaccg gcttcgcggc gggcttcgtg ctgctcgcgc
     1081 tgctcgatgc cttcgccggg ctgcggcttc ccgcgccggc gctggcgcgg aacgtcccgg
     1141 gatcgctggc gctcggcgtg agctcggagg tgcggctgcg catcgccaat gccgccgggc
     1201 tgcgcgtgcg cctggacttg cacgaccacc acccgcccag cttcgaagcc gaggggctgc
     1261 cgcggcggct cacgctcgag cgcgggcagt ggaccgaggt ggcttaccag gtgcgcccgg
     1321 tggcgcgcgg cgaaacgcag ttcagcgcgg ctgaagcccg gcttttttca ccgatagggc
     1381 tatggcagat ctcccgcaag attggcgccc caagcccggt tcgcgtctac cccaacttcc
     1441 gcgcgctggc gaagtacacg ctgctcgcca ccgacaaccg gctgtcgcag atcggcgtgc
     1501 tccaggtgcg caagcgcggc gagggcatgg agttccacca gctgcgggaa taccggcagg
     1561 gcgactcgca gcgcgccatc gactggaagg cgacctcgcg cacccagcgg ctgatcgcgc
     1621 gcgagtacga ggaggagaag gaccagcgcg tcatgctcgt catcgactgc ggacgacgca
     1681 tggcatcgaa ggacgatgag ctttcccatt tcgatcacgc cctgaacgcg gcgttgctgc
     1741 tagcgcacgt cgcgctgcgg cagggcgacg cggtcgggat gatgaccatg gcggggatca
     1801 accgcttcgt cgagccgcgc aagtccgtcg gcgcggtgaa cgccgtcctc aaccgcgtct
     1861 acgacatcga gccgacgctc gaggtgcccg actacaacct ggcggcgcgc gacgtgatgc
     1921 ggcggctgcg ccggcgctcg ctcgtcatcg tgctcaccaa cctgcgcgac gaggacgacg
     1981 agaccctgct gccggcgctg aagctcctgc gcacccggca cctggtcgtc ctggcctcgc
     2041 ttcgcgaagc catcatttcc cgggcgctct ccgcgcgggt cgacagcttc gaccgcgccg
     2101 tcacccacgc cgccgccgcc gagtacctcg ccgcgcgcga gcgcaccttc cgccgcatcg
     2161 gcgcctcggg cgccgtctgc ctggacgtcg agccggaacg gctcgccatc tcgctcgtca
     2221 accgttacct ggaattgaag cgcgagggtc gcctttaacc ccgaaaaaac ctttataatt
     2281 cgcgccctat gaaaccaggc atccaccccg actacaaagc ggtgatcttc ctcgacacca
     2341 gctcgaacca ctccttcctc acgcgctcgg cgatcgacac caagggccgc gagaccatga
     2401 agtgggaaga cggcaaggaa taccccgtcg tcaagatcga ggtgtcctcg gaatcgcatc
     2461 cgtactacac cggcaagcag aagatcgtcg acaccgccgg gcgcgtcgag aagttcaagc
     2521 gccgctacac caagaagact ccggagaaaa aggcctaact ggtctttggg ggatgctccc
     2581 ccgcctcagc ttacccccca ctccagcggc gctagcgctc attgcgctcg cgttcgcgct
     2641 tcccggcctc gccgggcacg acctgtggaa gacgcacgac gccatcggcc tcggcatcgt
     2701 gcacgacatg gccgagagcg gcgcgccgct cgtgccgcgc atcgcgggaa acccgtggct
     2761 gttcgacccg ccgctctacc actggctggc cctgagcttc ggcaaggcgc tgcaattcat
     2821 cctggagttc cattccgcag cgcggctggc gagcggcacg ctggtcctcg ccgctttctt
     2881 cctgatctac ctcgcggcgc gccgctgggc gcgtcccgac gacgatcgcc gcgtgcacgg
     2941 cgcggccgcg atgctcgtgc tcctgggctc gatcggcctg atcgtgcatg cgcacgaggc
     3001 gctgcccgag ctcgccgccc tcgccgccct gtgcggcgcg ctcgccgcgc tgccgcaggc
     3061 tgcgcgccgg ccgctgttgg ccggcgcgct cttcggcgcc gcggtcggca tcgctgcgct
     3121 ctccgcgatg tggatctcgc cggccgcgct gctcctcgcg gtgatcgccg cgcatgtgct
     3181 ctgtccgcag tggcgtacgc gaagcgcatg ggttttcgtc ccggtgtccc ttctcgcggc
     3241 actggcggtg gcggcgagct ggcccctcgc gctcgcgtgg cgcgagccgg aaacgttcgc
     3301 gctgtggcgc cacgtggcct ggcagccgat cggcgatccg gtggcgaacc tgcgctattt
     3361 cctcgtcacc gggagctggt ttgcctggcc ggcgtggccg ctggcggtgt gggcggcgtg
     3421 gtcgctgcgc cggcgctgga gcgagccccg gctcttcgtg ccggcggtgg caagcgtgct
     3481 cctccttctc tttgcggcgt actgggggcc ggcgcaggac gtgcacctga tcccgctgct
     3541 cgcgccgctc gcgctgctcg gctcccaggg cgcgctggtc ctgaggcgcg gcgcggcggg
     3601 cgcgctcgac tggttcggcg tgctcggctt cggcttcttc gggctgctga tctggttctg
     3661 ctggttcgcc atgctgaccg gagtgccggc gcgcttcgcc aacaacttct tcaagacggc
     3721 gcccggcttc tcgcccgagt tcaagctcgc gtacgtcgcg ttcgcgctgg tgctcgccgc
     3781 gggctgggtg gtcctgatct tctacaccac gcgctcgccg atgcgcagcg tgctgcgctg
     3841 ggcggccggc atcgtgctgc tgtggggcac gggggcgatg ctgctcatgc cctgggccga
     3901 ctatcagaag agctaccgct cggtggcgct gcagctgcgc tcgaaaatcg cggtcggctc
     3961 gggctgcatc gcacagaagg cgctcggcgt gtcgcaggcc gccgcgctcg actaccacgc
     4021 gggcatccgc gcgcgcccct acgacctggt gcggccgaac gcctgtccgc tgctgctcgt
     4081 gcagggcaac ccgctgcatg aattcgacgg gcccggcgcc ggctgggtca agcttgccga
     4141 cgtcggccgc cccggcgacc gcgccgagcg gtaccgccta tatcgactgg gtaaatgaat
     4201 acacgctccc cgacgcaatg cccggcctgg gcgaagctct cgcagcacgc cgaaagctgg
     4261 cgcgcggtgc acttgcgcga gctcttctcc gccgacgcgg cgcgcggccg gcagttcgtc
     4321 gccgagaccc ccggcctgcg ctacgactat tcgcgccagc gcctcggcgc aatcaccctg
     4381 cgcctgctcg cgcatctcgc cgccgagcgc ggcttcgccg aatggcgcga agcgctcctc
     4441 gccggcagcg aggtgaactc gaccgagaag cgcgccgcct ggcatacggc cttgcgggcg
     4501 ggcgatggcg ccccgcgcga ggtcaaggaa acgctcgctc gcatgaaaga gctcagcggc
     4561 aagctgagag cggaaaagcg gttcaaacgg gtcatcaacc tcggaaccgg cggctcggac
     4621 ctcgggccgc ggctcctcgc cgacgcattg agcgacaacg cgctggacgt gcgcttcgcc
     4681 gccaatgtcg atccgcgcga cctcgcccgc gcgctccagg gcgccgagcc ggcgagcacg
     4741 ctcctcatcg tcgcgtcgaa gacgttcacc acccaggaga cgatggcgaa cgcgctcgcc
     4801 gcgaaggaat ggggtgccaa ggcgttctac gcggtcactt cgaatgtgca ggcggcggag
     4861 gcattcggcg ccgccgagat cctgccgatg tgggattggg tcggcgggcg cttctcggtc
     4921 tggtcggcgg tggggttcgc ggccgcctgc gccatcggct tcgattcgtt ccagcagttc
     4981 ctgcagggcg gcaacgacat cgaccggcac ttcgccgaaa cgccgctgga aaagaacgtc
     5041 ccggcgctga tggcgctgat cggcgtgtgg aacgccaact tcctcggctc gccgacgcac
     5101 gcagtgctgc cgtacgcgaa cgcgctgcgc ctgctcccgg ccttcctgca gcagctcgag
     5161 atggagtcga acggcaagcg tgtcgatcgc gagggccgcg tcgtcgacta cgccaccgcg
     5221 ccggtgctct ggggcggcga aggcacggtg agccagcact ccttccacca gctcctgcac
     5281 cagggcaccc acgcggtccc ggccgatttc gtcgacgtcg gggaaagtcg cgttcttacg
     5341 gcaaaccttc gcgcccaggc cgacgcgctg gcgtacggca ccgacgatcc gaagctgccg
     5401 ccgcatcagc agtaccccgg caaccggccc tcgagtgtgc tgtcgctgga gaagatggac
     5461 gcgaggaacc tgggacgcct catcgccctc tacgagcaca aggtcttcac ccagggggtg
     5521 atctggaaca tcaacagctt cgaccagtgg ggcgtcgagc tcggcaagaa acttgcccag
     5581 aaaatcctta ccgaaggaaa aaacgaggag cgctaatgga tcaggaaacg ttcaatctga
     5641 gcatccgcaa gttcctgaag atggtcgggg tcaactcgca gcgcgagatc gagcaggcgg
     5701 tgcagaaatc cctgcaatcc ggcaagctga aaggcgagga aacgatcccg gtgaaagtga
     5761 tgctcgagat tccggcgctc ggcgtgaagg tgccgttcga cggcgagatc agactaaaat
     5821 aggtccgtga acaaggcacg agagttcgac caatccgtgg tcgccgagct gaaggcgctg
     5881 ctcggcgagc gggtgtcgac cgccgcggcg gtgcgcgagc accacggcaa ggacgagtcg
     5941 tactttccct acgcgccgcc tgatgttgtc gtattcgtaa gaacaaccga agaggtgcgc
     6001 gacgtcgtga acatctgccg ccgccaccgc gtgccgatga tcccctacgg cgtcggcacc
     6061 tcgctcgagg gccacatcct cgcgatcgcg ggcggcgtct cgatcgacct ttcgcagatg
     6121 aaccaggtgc tcgcggtgca cgagcaggac ctcgacgcgg tggtgcaggc gggcgtgacg
     6181 cgcaagcagc tgaacgagtt catcaagcac acggggctgt tcttcccggt cgatcccgga
     6241 gccgacgcga ccctcggcgg catggccgcg acgcgggcct ccggaaccaa tgcggtgcgc
     6301 tacggcacga tgcgcgagaa cgtgctgtcg ctgaaggtgg tgctcgccga cggccgcatc
     6361 atccagacct cgcggcgcgc gaagaaatcc gccgcaggct acgacctcac gcgcctgttc
     6421 gtcggctcgg aaggcacgct gggcatcatc accgaggtga cggtgcgcct gtacccggtg
     6481 caggaagcga tgtcggcggc ggtctgcgcc ttcgacagcg tggacggctg caccaacacc
     6541 gtgatccaga ccatccagtc cggcatcccg atcgcgcgct gcgacatcgt gtgcgagaag
     6601 accgtcgac
//
LOCUS       DATBSZ010000240        20133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1547483, whole genome shotgun sequence.
ACCESSION   DATBSZ010000240 DATBSZ010000000
VERSION     DATBSZ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20133
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547483"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VIG70_15155"
     CDS             <1..297
                     /locus_tag="VIG70_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEY8251937.1"
                     /translation="TAGTAYFRRTADKTLHDRFLPIVSTATEKVGVAQRYKEFARPAA
                     ALGLLKTEHADLDEYVTQKALEGLYFMLGEEEKKIRKDPVGTGSAILRKVFGAL"
     gene            633..1607
                     /locus_tag="VIG70_15160"
     CDS             633..1607
                     /locus_tag="VIG70_15160"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251938.1"
                     /translation="MNRRSAVAALLFTLAAPTRLAAQTARLPRIGYLTLAPLGDKASD
                     ERLAFLEGLRELGYVDGKTVEMVYRSGEMNLDILEFAAQALVDAKVDVIAASGTVPAL
                     AAKKLTRTIPIVMIFSSEPVLAGLVVDLARPVGNVTGVATIQTELDPKRLAMLKEISP
                     TITKVAVLWTRFHPSHRAELQAVEKAGQVLGLTMESFDVTDDLLGALRKMETRLPDAI
                     FVLWDVRTLNFRQFIIDFALKHNLPTSMPLEPYVEAGGLISYGPNVQSIFRRSATYVH
                     RILQGAKPADLPVERPSKFDLVVNLKTAEKLRLKLPHALLLLADRVIQ"
     gene            1646..3736
                     /locus_tag="VIG70_15165"
     CDS             1646..3736
                     /locus_tag="VIG70_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEY8251939.1"
                     /translation="MKTIPLALAAGVLALAFCGPVDARVTRIVIDEVLPMPAGSPAGA
                     IAYEQVAGRAFGELDPKLPGNAIIQDIELAKDADGKVRYVATFLISKPVDVSRASGLM
                     WHNVPNRGRPVALPAQERAFGDIVLDSAWQGDNSGWTAVRDRASVAGNHFLQVAVARG
                     AGGAPITGEVFGRIANRSGMGSQPLIVLFNPVPYKPVSLDTTRSKLVSRGRENQLGEV
                     FDEIAVPASDWAWARCDANNPFPGVPDPAQICLKNGFDGTRLYQVVFTAADPHVLGIG
                     FAAWRDVGAFFKKAAADDAGTRNPIAKAVTHSIARGVSQSGNYLRGWLHLGFNRAEDG
                     GMVHDGMWPIIAGRRIALNFRWAQPDGAMELYQAGSEGPQWWSPHPDPVRGHPSAGIL
                     DRCTATRTCPKVIEHFGSAEVWALKLTPEWVGADAKADLPLPDTVRRYYIASTTHGGG
                     AGGFDTSLPGVGLPKTGVACPGNNFGTGVLPANPVPHAQTVNAIRVHFRDWVMKGVPP
                     PPSRYPTLARGQLAESHKAAIGFPTLPGLRATVPEPDFIMRVHDYDWGPQFNAVDGSG
                     IASLAPPRIRQSLKMLAPKVDADGNEVGGVPVVLLDAPLGTYLGWNVAAGGFHKGQIC
                     YYAGGMIPFARNAEERRANHDPRPSLEERYGSHEGYVAAVKKAAARAMAERFLLQEDA
                     AALIAAAEKSAVLR"
     gene            complement(3750..4790)
                     /locus_tag="VIG70_15170"
     CDS             complement(3750..4790)
                     /locus_tag="VIG70_15170"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8251940.1"
                     /translation="MGELKSLGRYQIQGVLGKGAMGLVYDGHDPKLNRRVAIKTILTA
                     TLDEATARHYTVRFKREVRAVARLNHPNIVQVYDFGNEGDLAYIVMEYIKGKELKDHF
                     EKKERFDLKTIFRLMTELLDALECAHEAGVIHRDIKPANLMIDAGGHAKLTDFGVARF
                     TEPDGEQLEATRAGTIVGTPSYMSPEQIQGQTLDRRTDIFSAGVVFYQLLTGQKPFEG
                     TQWALAKKIVQDDPVWPSSLVQIPTAIDRVVARALAKAAEQRYQTARSFATSLKRILE
                     GKPPEDPDEVLPAAASPAPRTVSSEAETEFWNEVKDSDDPEDLALYVEQFPQGAYVEL
                     AKRKIAELGKKR"
     gene            complement(4795..7509)
                     /gene="polA"
                     /locus_tag="VIG70_15175"
     CDS             complement(4795..7509)
                     /gene="polA"
                     /locus_tag="VIG70_15175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY8251941.1"
                     /translation="MSGSNPEKTLLLVDGSSYLYRAFHALPGLKSPQTGEPTGAIYGV
                     LNMLRKLATDYKPAALACVFDAKGKTFRDAEYSEYKANRTPMPDDLRVQVAPLHEAVE
                     ALGWPMLIVEGVEADDVIATLAEEAKRDGWRAVISTGDKDFAQLVDERVTLVNTMSNE
                     LLDIEGVKKKFGVPPEKFLDYLTLIGDQIDNIPGVDKVGPKTAAKWIEKYGSLEGVLA
                     HADEISGLAGENLRKARDWLPKARHLLTIKRDVPLDVKIENLQKKPDPARQREQYERF
                     GFKSWLKEFEGLASAPAAASPAAATAMQKRRHRTILTEDELKDLLLKIEAADLVGFDT
                     HCVGEEPMTARIVGLSFAFGDEAVYLPLAHEYAGAPAQLAVDAALELLKPWLERDDRR
                     KVGEDVKLDTHALANHGVRLAGCVHDTLLESYVLEVHERHELGMLAQRYCGWTILGYD
                     EVTGKGAGRIPFSAVDVARATEYAAGKTDCALALHGLLFEKIESDEKLKFVYGSIEMP
                     VLPVLWRMERNGVLLDREKLDAQSHELGKEMLQKEQDAYSAAGQPFNLGSPKQIQEIL
                     FDRLKLPVKKKTPSGQPSTDEDSLAELALDHPLPKLILEHRTLSKLKSTYTDKLPRSI
                     NAKTGRVHTTYSQTTAVTGRLSSNEPNLQNIPIRTPAGRRIRECFIASPGSKIVSADY
                     SQIELRIMAHLSGDANLRRAFAEGEDVHRATAAEVFGVPLSAVEPDHRRVAKVINFGL
                     IYGMSSFGVAQNLGIDRGTAQIYVDRYFARFPGAKRYMDETRARARELGYVETVFGRR
                     LWLPELKSGAPVRRQAAERAAINAPMQGTAADLIKLAMISVQGFLEKEKLKAMLIMQV
                     HDELVLEVPVGELETVKTKVPELMQSVAKLEVPLIVEVGVGDNWDKAH"
     gene            7508..8224
                     /locus_tag="VIG70_15180"
     CDS             7508..8224
                     /locus_tag="VIG70_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY8251942.1"
                     /translation="MNQDRQPKLAELAKEVPAPEAWRVFGIMAELVEATERLVGIHPA
                     VSVFGSARVKPGTPYYELAEETARLLSDAGFAVVCGGGPGLMEATNRGAFKGAGLAVG
                     LNIDLPMEQPSSRYQHISLRFKHFFTRKAMFAKVATAFIVLPGGYGTLDELFEALTLV
                     QTGKTRKMPIILVHEPYWRGLLTWMRERLAAEGMIDAGDLDLVQVYDKPQQVVDAIFA
                     FYEKRGFAPSPAEREVLLNL"
     gene            8238..8567
                     /locus_tag="VIG70_15185"
     CDS             8238..8567
                     /locus_tag="VIG70_15185"
                     /inference="COORDINATES: protein motif:HMM:NF022639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HEY8251943.1"
                     /translation="MRRLLLVGFLVIFSTLSHAQSRPPKLEPLPEPPPPPPMAPEEPT
                     VRIPVETGDKIEEVREGGRVVMLKVTPPHGKPYYLVDTTGNGNWMRRDSLDDGVRVPM
                     WPIHTWD"
     gene            8568..9518
                     /locus_tag="VIG70_15190"
     CDS             8568..9518
                     /locus_tag="VIG70_15190"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY8251944.1"
                     /translation="MSVYTPVSEVELDGWLRNYSVGRLTACEPIKAGIENTNYFVTTT
                     QGRYVLTLFERLPAEELPFYLHLMAHLARHGIPCPAPIADLSDQYLGSLNGKPAALVT
                     RLSGGSLEKPEAAHCEELGALLGRMHLAGRSYSAYLENPRGPKWWRFAAREVRSFLNK
                     KEVEMVDEELQYQAEQRFPDLPRGPVHADLFRDNALWEGARISGVVDFYFAGVDCLLY
                     DVAVCANDWCLKDLKADQGLDPARMKALLGAYHALRPFTALEREAWPAMLRAAALRFW
                     LSRLYDFHLPRPGMLVHAHDPEHFRRVLESRIAVPTPWIA"
     gene            9518..10273
                     /locus_tag="VIG70_15195"
     CDS             9518..10273
                     /locus_tag="VIG70_15195"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEY8251945.1"
                     /translation="MQARIVETRKGVRWLVDGWRLFRAAPLGWIAMVFAYWLIMTLVS
                     VVPLVGVAAASILVPAFSVGFMAAARAAASRGPVELAQLFDGFRHHLRSQMVLGVVYL
                     ACLALLLAATALADEGALATWMLAGRKPEDEVLQSEEFLTALVAAAALYAPVLMMFWF
                     APLLAAWHGAGPAKAVFFSFFACLMNWRAFLAYGAVTALLTLALPLAVLGIVTLASLK
                     VAPMALVFPLLIVLLPTLFASFYASYRDVFGAE"
     gene            10270..13986
                     /locus_tag="VIG70_15200"
     CDS             10270..13986
                     /locus_tag="VIG70_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HEY8251946.1"
                     /translation="MNRIREIPYNYTSFSDREITLRLLGPDGWALIAELRDERRTGRS
                     SRMLYEVLGEIWAIQRNPYLEDELVNRPQRRAEALGGMRRRLAEIEARRAGSLKVARL
                     LELTRAAVDGFEEEFAVTRALRAKAMRRLARVTRRDNILFDGFARVSHVTDATDWRVE
                     VPFVVLTPDSEEEIQPLVRELIALGLTIIPRGGGTGYTGGVIPLTRRSAVINTEKLES
                     LGPIEHAELPGVARPQPTIRASAGVVTRRVMDLAEEGGLVFAVDPTSADASCIGGNVA
                     MNAGGKKAVLWGTAIDNLVWWRMVTPQGEWLEVERLEHNLGKIHDAPKVRFGLKYFQG
                     KKLKQERVLEIPGSHFRKAGLGKDVSDKFLAGLPGVQKEGTDGIIVAARFLLHRMPAH
                     TRTVCLEFFGLARDAVPAIVEVKNYIEKSKAVLLAGLEHLDERYVKAVGYATKSRRSQ
                     RPRMVLIGDLVGDDVDAVAAAASGVVRIANARGGEGFIAVSPEARKRFWLDRARTAAI
                     AKHTNAFKINEDVVIPLEKLGEYTDGVERINIEFSVRNKLELLDALEEYFSGELAVED
                     RDLLGERPGRALELLRRVRGRWQAIASDMDHYFDELQSYRQRLSWKTEVRDELRHIFD
                     GVAFRRILEGTEEAHKRILRGRVFVALHMHAGDGNVHTNIPVNSDDYQMLQRASDAVA
                     RIMRLARSLGGVISGEHGIGITKLEFLEPAEIEAFRAYKQAVDPEGRFNAGKLLAGGD
                     LADAYTPSFSLIGAESLILDETDLGRVAGAIKDCLRCGKCKPPCATHVPRANLLYSPR
                     NKILATSLLIEAILYEEQTRRGVALAHYDEFGDVADHCTVCHKCEPACPVDIDFGDVS
                     IIMRNYLRRAGKRRFNPGTKASMLFLGLTDPRAVKMARTVMIDWGYRAQRAAFSLFKT
                     FGVVRKQTAAPPLTTGQKPLRAQVVHFINKPMPGRLPKKTARALLDIEDAKVVPIIRD
                     PAKVNEDSDAVFYFPGCGSERLFSQVGLATQAMLYEVGTQCVLPPGYLCCGYPQTAAG
                     ESEKGERIIVQNRVLFHRLATTLNYLDIRTVIVSCGTCLDQLEKYEFGAIFPGARLLD
                     IHEYLLEKGVKANDVPGVKYLYHDPCHTPIKTAKPLSVVNGLIGEARLSERCCGESGT
                     LAMTRPDISTQVRFSKEESLRKGIADLGGDASPVKILTSCPSCLQGLARFEDDTGAQA
                     DYIVVELARRLLGENWLPEFVAKANAGGIERVLL"
     gene            13983..14339
                     /locus_tag="VIG70_15205"
     CDS             13983..14339
                     /locus_tag="VIG70_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEY8251947.1"
                     /translation="MIERVPTGITLHQKSRVLEIAFADGRTFRLPYEFLRVYSPSAEV
                     RGHGPGQEVLQIGKRNVEIRSLEPVGSYAVQPQFSDGHSTGIYSWDYLYELGESQESL
                     WSQYLERLKAANASRE"
     gene            14323..15024
                     /locus_tag="VIG70_15210"
     CDS             14323..15024
                     /locus_tag="VIG70_15210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8251948.1"
                     /translation="MQAGNKADTVKRVFDSVAERYDVMNDLMSLGLHRAWKAFAVSIA
                     RPKPGERMLDIATGSGDLALALSRRVLPGGEVWITDINRRMLERGRDRLLDRGLALPA
                     VQCDAERLPFPSAHFDCVTVGFGLRNMTRKDAALAEMARVLKPGGRLVVLEFSKVWKP
                     LERAYDWYSFKVLPWLGDRVAGDADAYRYLAESIRLHPDQGALAEMLRAAGLADVEVF
                     NLAAGVVAVHRGVRF"
     gene            15053..15868
                     /locus_tag="VIG70_15215"
     CDS             15053..15868
                     /locus_tag="VIG70_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HEY8251949.1"
                     /translation="MSKWLLTAVIIGFGGIFVATEVEARRLGGGRNMGAQRNVTAPPA
                     QTPAKQQQAAPAQQQPGQATPAAARTPWAGILGGLALGGMLGYLFGGSGLMGFLLLAL
                     LAIAAVVVVRALMQRRQETSRPVQFAGMRETVNVTPDSGGGQMPIAKSNVPAGFDAGS
                     FLRAAKLNYLRLQAANDAGRMEDIREFTTDELFEALKSELGERSQTEVAGLEAELLEV
                     ATEGKEHWASVRFSGQVREAPGAAPEAFAEVWNLVKPADGSSGWLLAGIQQMH"
     gene            15922..16458
                     /locus_tag="VIG70_15220"
     CDS             15922..16458
                     /locus_tag="VIG70_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251950.1"
                     /translation="MNRLLAAEPWARLRLAPFAGETVELRAPLLPTLRLRILAGGTVE
                     PGGAEPGLTMTLKPELLAALARGEEHAMRSVDVEGDPKLAAEVLVLARHLRWDAEEDL
                     SRLVGDVAAHRLAQAGRAFAAWHLDAAQRLAAAVADYVTEEKQLLARRADLVTLGDSV
                     ARLRDAVARLDKRVERLG"
     gene            16451..17971
                     /gene="ubiB"
                     /locus_tag="VIG70_15225"
     CDS             16451..17971
                     /gene="ubiB"
                     /locus_tag="VIG70_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEY8251951.1"
                     /translation="MARVTRLARIVAIGLRFGLHEFVPRFSRNLLVRAFAGHKGEPRG
                     QRLREALETLGPIYVKFGQVLSTRRDLLPLDIADELARLQDRVPPFGSDLAVAEVERS
                     LGKPLDVLFSSFERTPVASASIAQVHLAVLHDGAEVAVKVLRPGVEAAIARDLELLET
                     GAGLAERLLVDGRRLKPREVVAEFARHLDDELDLMREAANASQLGRNFAGSPLLLVPA
                     VHWDLCAQRVMVMERMHGTPISQVEVLRQKGIDIPALARAGVEIFFTQVFRHGFFHAD
                     MHPGNILVATEEEMRGRYIALDFGIMGTLTEVDKNYLAQNFLAFFNRDYRRVAQAHLD
                     AGWVPAGTRVDEFEAAIRAVCEPIFARPLREIYFGKLLLRLFQTSRRFNVEVQPQLVM
                     LQKTLLNIEGLGRELDPDLDLWQTAKPFLERWMREQVGWRGLVRTIRHEAPFWAATLP
                     QLPRLVHRALAEDRLGALHTAVERLADEHARRNRLLSVVLVALVVWLAATLATLFL"
     gene            17983..18573
                     /locus_tag="VIG70_15230"
     CDS             17983..18573
                     /locus_tag="VIG70_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY8251952.1"
                     /translation="MAQDSSRPEFWDTRFRDGVTPWDAGGVPPALKNWIGRRSGRLRV
                     LIPGAGTGYEVRLFAGHGHDVLAIDFSDAAIEAAKRELGSLSGRVRKADFFGLQDGPF
                     DVVYERAFLCALPRTLWSEWARRMAELVSPGGVLAGFFYLDDNQRGPPFGVSQEGLKD
                     LLGKTFEMTESRVLPPEQSLPVFQGKEIWQVWTRRL"
     gene            18584..20026
                     /locus_tag="VIG70_15235"
     CDS             18584..20026
                     /locus_tag="VIG70_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY8251953.1"
                     /translation="MLIGSVALYLALTIAIGLWAAKRVHNAKDYVVAGRSLPLYMSAA
                     TVFATWFGAETVLSVSATFAKDGLGGIVADPFGASFCLLFVAIFFARAFYRMDLLTIG
                     DFYRKRYGKTVEVITSVAITASYLGWTSAQLTALGLVFSVLSGGAISLSTGILIGAGI
                     VVFYTIFGGMWSVALTDLFQSVVILVGLVAVAWLVGDMAGGAGKVIAAASEAGKFEFW
                     PKGGTKEWLWFLAAWMTLAIGSIPQQDVFQRVTAAKDEKTAVRGTLIGGFVYFCFAFV
                     PIFIAYAALVIDPGYEKLFAGEDAREIQRILPDLILNRTPVWAQVLFFGALLSAILST
                     ASGALIAPTSLFTENVIRPFFRGMSDRQFMLLLRVILVTFTLAALLFALNSKSTMYEM
                     VQNAYKVTLVSCIVPLAAGIFWKRANVPGAILSVVFGLASWTIAEWVAADATVPPQLV
                     GLGFSLVGMVLGSLVPSAAVQPAAHHHGKH"
BASE COUNT         3163 a         6653 c         6746 g         3571 t
ORIGIN      
        1 accgcgggca cggcgtattt ccgccgcacc gccgacaaga cgctgcacga ccgcttcctc
       61 ccgatcgtaa gcacggcgac cgagaaggtc ggcgtcgccc agcgctacaa ggagttcgcg
      121 cgaccggcgg ccgcgctcgg cctgctcaag accgagcacg ccgacctcga cgagtacgtg
      181 acgcagaagg ccctcgaggg gctttacttc atgctcggcg aggaagagaa gaagatccgc
      241 aaggatccgg tcggcaccgg cagcgcgatc ctgcgaaagg tgttcggcgc tctctagcgg
      301 gtgcgcaatc cctcgtctgc ccgtcctcca agccggctat cctcttatgc gcatgcgtgt
      361 gcccgcgaat ctttgcccca agcagaggtg ttcagtaacc taaacgcttg agcggaaagc
      421 gtggagtact cacgcaaccc agaaatgccc gcttgcatga catttacgcg gttcactaca
      481 cgttaggtgg cacatcggtc gcacaatgcc ggagcaggtg aaagcgttct tattgtcctt
      541 aacttttccg ggcgaaagcg tagagttcga gcacggttcg ttgcgaaggc ggcgcatgag
      601 cctcgggacg ggcaggtgcg gagcgaaagg gaatgaatcg ccgtagtgca gtcgcagccc
      661 tgcttttcac gctcgccgca ccaacgcggc tcgctgcgca gaccgcacgt ctcccgcgca
      721 tcggttacct gactctcgcc ccgcttggcg acaaggcctc ggatgaacgc ctagcctttc
      781 tggaaggctt gcgtgaactg gggtacgtcg acggcaagac cgtcgagatg gtttaccgct
      841 ctggcgagat gaacctcgac attctcgaat ttgccgcgca ggctttggtc gacgcgaagg
      901 tcgacgtgat cgccgcctcc gggacggtcc cagccctcgc cgcgaagaaa ctgactcgga
      961 cgattccgat cgtgatgatc ttttcctccg aacccgtgct cgcgggtttg gtcgtagacc
     1021 tcgccaggcc cgttggcaac gttacaggtg tggcaaccat tcagactgag ctcgacccga
     1081 agcggcttgc gatgttgaaa gagatcagtc ccacgatcac gaaagtggcg gtgctctgga
     1141 cgcgctttca cccatcccac cgcgccgaac tgcaggccgt cgagaaagcg ggccaggtgc
     1201 tgggtcttac aatggagtca tttgatgtga cggacgactt gctcggcgcc ctccgcaaaa
     1261 tggaaacacg gctgccggat gcgatttttg tcctttggga cgtgcgcacc ctcaacttcc
     1321 gccagttcat catcgatttc gccctcaagc acaacctgcc gacgagcatg cccctcgagc
     1381 cttacgtcga agcgggcggt ctgatctcct atggcccgaa cgtacagtcc atcttccgcc
     1441 gcagcgccac ctacgtgcac cgaatcctac agggcgcgaa acccgccgac ctcccggtcg
     1501 agcggccgtc gaagtttgat ctcgtggtga atctgaagac cgccgaaaag ctgagactca
     1561 agctgcctca cgccctcctg ctgctcgcgg accgggtgat tcaatgacga gttgtggccg
     1621 cttgcggccc gtcgaggagg acaacatgaa gaccatcccg ctcgcgctgg ccgccggcgt
     1681 attagcactt gcgttttgcg gcccggtcga tgcgcgtgtg acgcgcatcg tcatcgacga
     1741 agtgctgccc atgcccgcgg gcagcccggc tggagccatc gcctacgagc aggtggcggg
     1801 gcgcgctttc ggcgagctcg acccgaagct ccccggcaac gccatcatcc aggacatcga
     1861 actggccaag gatgccgacg gcaaggtgcg ctatgtcgcc acgttcctga ttagcaagcc
     1921 ggtggacgtg agccgcgcca gcggactgat gtggcacaac gtgcccaatc gcggacgccc
     1981 ggtggcgctg cccgcgcagg agcgcgcctt cggggacatc gttctcgaca gcgcctggca
     2041 gggcgacaac agtggctgga ccgcggtacg ggacagggcc agcgtcgccg gcaaccattt
     2101 cctgcaagtg gcggtcgcgc ggggcgcggg cggcgcgccg atcaccggcg aggtattcgg
     2161 gcgcatcgcc aatcgttcgg gcatgggctc gcaacccctg atcgtgttgt tcaatccggt
     2221 gccgtacaag ccagtgagcc tggatacgac caggtcgaag ctggtatcgc gcggccgcga
     2281 aaaccagctg ggagaggtat tcgacgaaat cgccgttccg gcgagcgatt gggcgtgggc
     2341 acgctgcgac gcgaacaatc cattccccgg cgtgcccgat ccggcgcaaa tctgcctgaa
     2401 aaacggcttc gacggcacgc ggctgtacca ggtcgtgttc accgccgccg atcctcatgt
     2461 gctgggcatc ggcttcgccg cgtggcgcga tgtcggcgcg ttcttcaaga aagccgccgc
     2521 agatgacgca ggcacgcgga acccgatcgc caaagccgtc actcacagca tcgcgcgcgg
     2581 cgtttcgcaa tcgggaaact acctgcgcgg ctggttgcac ctcggcttca accgggccga
     2641 agacggcggc atggtgcacg acggcatgtg gccgatcatc gccgggcgcc gcatcgcgtt
     2701 gaatttccgc tgggcacagc ccgatggcgc gatggagttg tatcaagcgg gcagcgaagg
     2761 accgcagtgg tggtcgccgc atccggaccc ggttcgcggc catccgtcgg cgggaattct
     2821 cgatcgctgt accgcgacac gaacctgccc gaaggtgatc gagcatttcg gttctgccga
     2881 agtgtgggca ctcaagctca cgccggaatg ggtgggcgcg gacgccaaag ccgatttgcc
     2941 actgccggac acggtgcgcc gctactacat tgccagtacg actcacggcg gcggcgcagg
     3001 cgggttcgac accagcctgc ccggcgtcgg gctgcccaag acgggagtcg cctgcccggg
     3061 caacaacttc ggcaccgggg tgctgcccgc caatcccgtg ccgcatgcgc aaaccgtgaa
     3121 cgccattcgc gtgcatttcc gcgactgggt gatgaaaggc gtgccgccgc cgcccagccg
     3181 ctatccgacc ttggcgcggg gccagctggc cgagtcacac aaggcggcca tcgggtttcc
     3241 cacgctcccc gggctgcgcg ccaccgtgcc ggagccggac ttcatcatgc gagtgcacga
     3301 ctacgattgg ggcccgcagt tcaatgcggt cgatgggtcc ggcatcgctt cgctggcgcc
     3361 gccgcgcatc aggcagagtc tgaagatgct ggcgcccaag gtcgatgccg atggcaacga
     3421 agtcggcggc gtgccggtgg tgctgctcga cgcaccgctc ggcacctacc tgggctggaa
     3481 tgtcgcggcg ggcggatttc acaaaggcca gatctgctac tacgccggcg gcatgattcc
     3541 cttcgcacgc aacgccgagg agcgacgcgc caaccacgac ccacgtccgt cgctagagga
     3601 acgctacggc tcccacgagg gctacgtggc ggcggtgaag aaagccgcgg cacgcgcgat
     3661 ggcggaacgg ttcctgctgc aggaagatgc cgcggcgctg atcgctgcag cggagaaaag
     3721 cgcggtgctg cgctgatgcg tgagcgactt cagcgcttct tgcccagctc ggcaatcttg
     3781 cgtttcgcaa gctcgacgta tgcgccttgc gggaactgct cgacgtacag cgcgaggtcc
     3841 tcgggatcgt cgctgtcctt gacctcgttc cagaattccg tctcggcttc gctgctgaca
     3901 gtcctgggcg caggcgatgc cgccgcgggg aggacttcgt cagggtcctc cggcggcttg
     3961 ccttcgagga tgcgtttgag cgaggtggcg aagctgcgcg cggtctgata acgctgctcg
     4021 gcggccttgg cgagggcgcg ggcgacgaca cggtcgatcg cggtcggaat ctgcaccagc
     4081 gaggacggcc agaccggatc gtcctggacg atctttttcg ccagcgccca ctgcgttccc
     4141 tcgaacggct tttgccccgt gagcagctgg tagaagacca ccccggccga gaagatgtcc
     4201 gtgcgccggt cgagcgtctg cccctggatc tgctcggggg acatgtagga cggcgtgccg
     4261 acgatcgtgc cggcgcgcgt cgcctcgagc tgctcgccgt cgggctcggt gaagcgcgcg
     4321 acgccgaaat cggtgagctt ggcgtggccg ccggcgtcga tcatcaggtt ggccggcttg
     4381 atgtcgcggt ggatgacgcc ggcctcgtgc gcgcactcga gcgcgtcgag cagctcggtc
     4441 atcaggcgga agatggtctt gaggtcgaag cgttccttct tttcgaaatg gtccttgagc
     4501 tccttgccct tgatgtactc catcacgatg tacgcgaggt cgccctcgtt gccgaagtcg
     4561 tacacctgga cgatattggg gtggttgagc cgcgcgacgg cgcgcacctc gcgcttgaag
     4621 cgcaccgtgt aatggcgcgc ggtcgcttcg tcgagtgtgg cggtcaggat ggtcttgatc
     4681 gccacccggc ggtttagctt ggggtcgtgg ccgtcgtaga ccaggcccat cgcgcctttc
     4741 ccgagcacgc cctggatctg gtagcggccc agcgatttca gctcgcccat cgcgctagtg
     4801 tgccttatcc cagttgtcgc cgacccccac ctcaacgatg aggggcacct cgagcttggc
     4861 caccgattgc attagctccg ggactttcgt cttcacggtc tcgagctcgc ccacgggcac
     4921 ctcgagcacg agctcgtcgt gcacctgcat gatcagcatt gccttcagct tctccttctc
     4981 caggaaacct tgcactgaaa tcatggcgag cttgatcagg tccgccgcgg tgccctgcat
     5041 cggcgcgttg atcgcggcgc gctcggcggc ctggcggcgc accggcgcgc cgctcttgag
     5101 ctcgggcagc cacagccgcc ggccgaatac cgtctcgacg tagccgagct cgcgcgcgcg
     5161 ggcgcgcgtc tcgtccatgt agcgcttcgc gcctggaaag cgcgcgaagt accggtctac
     5221 gtagatctgc gcggtgccgc ggtcgatgcc gaggttctgc gccacgccga aggacgacat
     5281 gccgtagatc aggccgaagt tgatcacctt cgctacgcgt cgatggtcgg gttctactgc
     5341 agacagagga accccgaaga cctcggcggc agtcgcgcga tggacatcct cgccctcggc
     5401 gaacgcgcgc cgcaggttcg cgtcgcctga gaggtgcgcc atgatcctga gctcgatctg
     5461 cgaataatcg gccgacacga tcttcgagcc cggcgacgcg atgaaacact ctcggatgcg
     5521 ccgtccggcc ggcgtgcgga tcggaatgtt ctgcaggttg ggctcgttgg acgagagcct
     5581 gccggtgacc gcggtggtct gcgagtaggt cgtgtgcacg cggccggtct tcgcgttgat
     5641 gcttctgggc aatttgtccg tataggtcga cttcagtttc gagagcgtgc ggtgctcgag
     5701 gatcagcttc ggcagcggat ggtcgagcgc gagctcggcg agcgagtcct cgtcggtcga
     5761 aggctggccc gacggcgtct tcttcttgac cggcagcttc agccggtcga acaagatctc
     5821 ctggatctgc tttggcgagc cgaggttgaa gggctggccc gctgcggaat aagcgtcttg
     5881 ttctttttgt aacatctctt ttccgagctc gtggctctgc gcgtcgagct tctcccggtc
     5941 aagcagcacg ccgttgcgct ccatgcgcca gagcaccggc agcaccggca tctcgatcga
     6001 gccgtagacg aacttcagct tctcgtcgct ttcgatcttt tcgaaaagca agccatgaag
     6061 cgcgagcgcg cagtcggtct ttccggcggc gtactcggtg gcgcgcgcga cgtcgaccgc
     6121 ggagaacggg atgcgcccgg cgcctttgcc ggtcacttcg tcgtagccga gaatggtcca
     6181 gccgcagtag cgctgcgcga gcatgccgag ctcgtggcgc tcgtgcactt ccagcacgta
     6241 ggattcgagc agcgtgtcgt gcacgcagcc cgcgaggcgc acgccgtgat tcgcgagcgc
     6301 gtgcgtatcg agcttcacgt cctcgccgac cttccggcgg tcgtcgcgct cgagccaggg
     6361 cttgagaagc tcgagcgccg catcgaccgc gagctgcgcc ggcgcgcccg cgtactcgtg
     6421 cgcaagcggc aggtaaaccg cctcgtcgcc gaaggcgaac gacaggccga cgatgcgcgc
     6481 cgtcatcggc tcctcgccca cgcagtgggt gtcgaagccc acgaggtccg ccgcttcgat
     6541 cttgagcagc aggtccttca gctcgtcttc cgtgaggatc gtgcgatgcc tgcgcttctg
     6601 catcgccgtt gccgcggccg ggctggcggc cgcgggcgcc gacgcaagcc cctcgaattc
     6661 cttcagccag gacttgaagc cgaagcgctc gtactgctcg cgctggcgcg cggggtcggg
     6721 cttcttctgc agattctcga tttttacgtc gagaggcacg tcacgcttga tggtgaggag
     6781 gtggcgcgcc ttcgggagcc agtcgcgcgc cttgcgcagg ttctcaccgg ccagcccgct
     6841 gatctcgtcg gcgtgcgcga gtacgccttc gagcgagccg tacttctcga tccacttggc
     6901 cgcggtcttc gggccgacct tgtcgacgcc gggaatgttg tcgatctggt cgccgatgag
     6961 ggtgaggtag tcgaggaatt tctccggcgg cacgccgaat ttcttcttca cgccctcgat
     7021 gtcgagcagc tcgttggaca tggtgttgac gagcgtcacg cgctcgtcca ccagctgcgc
     7081 gaagtccttg tcgccggtcg agatcaccgc gcgccagccg tcgcgcttcg cctcctcggc
     7141 gagcgtggcg atcacgtcgt cggcctcgac gccttcgacg atgagcatcg gccagccgag
     7201 cgcctcgacc gcctcgtgca gcggggcgac ctgcacgcgc agatcgtcgg gcatgggcgt
     7261 gcggttcgcc ttgtactcgg agtactcggc gtcgcggaag gtcttgccct tggcgtcgaa
     7321 cacgcatgcg agcgccgccg gcttataatc ggtcgcaagc ttgcgcagca tgttgagcac
     7381 gccgtagatc gcgccggtcg gctcgccggt ctgcgggctc ttcagcccgg gcagcgcgtg
     7441 gaatgcgcgg tacaggtacg acgagccgtc gaccagcaat aacgttttct caggattact
     7501 tcccgacatg aatcaagaca ggcaaccgaa gctggccgag ctggcgaaag aggttcccgc
     7561 gcccgaggcg tggagggtgt tcggcattat ggcagagttg gtcgaggcga ccgagcgcct
     7621 ggtcggcatc catccggcgg tgtcggtgtt cggcagcgcg cgcgtcaagc ccggcacgcc
     7681 gtactacgag ctcgccgaag agaccgcgcg gctgctttcg gatgccggct tcgcggtggt
     7741 gtgcggcggc gggccggggc tgatggaagc gaccaaccgc ggcgcgttca agggcgccgg
     7801 cctggcggtg gggctcaaca tcgacctgcc gatggagcag ccctctagcc gctaccagca
     7861 catcagcctg cgcttcaagc acttcttcac gcgcaaggcg atgttcgcca aggtcgcgac
     7921 cgctttcatc gtgctgcccg gaggatacgg cacgctcgat gagctgttcg aggcgctcac
     7981 gctggtgcag acgggcaaga cgcgcaagat gccgatcatc ctggtgcacg agccctactg
     8041 gcgcggcctg ctcacgtgga tgcgcgagag gctcgccgcc gagggcatga tcgacgctgg
     8101 cgatctcgac ctcgtgcagg tgtacgacaa gccgcagcag gtcgtcgatg cgatcttcgc
     8161 tttctacgag aagcgcggct tcgcgccgtc ccccgcagag cgagaggtgc tgttgaacct
     8221 ctagtagaat cgacgcgatg cggcgcctgc tgttggtagg gtttctcgtc attttttcga
     8281 cgctgtcaca cgcgcaaagc cgcccgccca agctcgagcc gctacccgag ccgccgcctc
     8341 ctccgccgat ggcgccggag gagcccacgg tgcgcatccc ggtcgagacc ggcgacaaga
     8401 tcgaggaagt gcgcgagggc ggccgcgtag tgatgctcaa ggtcacgccg ccgcacggca
     8461 agccctacta cctggtcgac acgaccggca acggcaactg gatgcgccgc gattcgctcg
     8521 acgacggcgt gcgggtgccg atgtggccga ttcacacgtg ggactgattg tcggtttaca
     8581 cgcccgtttc ggaggttgag ctcgacggct ggctcaggaa ctattcggtc ggccggctga
     8641 cggcgtgcga gccgatcaag gccggcatcg agaacaccaa ctacttcgtc accaccacgc
     8701 agggccgcta tgtcctgacg ctgttcgagc ggctgccggc ggaggagctg ccgttctacc
     8761 tgcacctcat ggcgcacctc gcgcgccacg ggattccgtg cccggcgccg atcgccgatc
     8821 tctccgacca gtacctcggc agcctcaacg gcaagcccgc ggccctcgtc acgcggctgt
     8881 ccggaggctc tctcgagaag ccggaggcgg cgcactgcga ggagctcggc gcgctcctcg
     8941 gccgcatgca cctcgccggc cgctcttata gcgcctacct ggagaatccg cgcggtccga
     9001 agtggtggcg cttcgcggcg agggaagtca ggagtttctt gaacaaaaaa gaagttgaga
     9061 tggtggatga agagctgcaa taccaggcag agcagcgctt tccggatctg ccgcgcgggc
     9121 cggtgcacgc ggacctcttt cgcgacaacg cgctgtggga gggtgcgcgc atttccggcg
     9181 tggtcgactt ctatttcgcc ggcgtcgact gcctgctcta cgacgtcgcg gtgtgcgcca
     9241 acgattggtg cctcaaggat ctgaaggcag atcagggcct cgatccggct cggatgaaag
     9301 ccctgctcgg tgcctatcat gcgttgcggc cgttcaccgc gctcgagcgc gaggcctggc
     9361 cggcgatgct gcgcgccgcg gcgctgcgct tctggctgtc gcgcctgtac gacttccact
     9421 tgccgcgccc gggcatgctg gtgcacgcgc acgacccgga gcacttccgc cgggtccttg
     9481 agtcgcgcat cgcggtcccg actccctgga tcgcctgatg caggcgcgga tcgtggagac
     9541 gcgaaagggc gtgcgctggc ttgtcgacgg ctggcgcctg ttccgtgccg cgcccttggg
     9601 atggatcgcg atggtgttcg cgtactggct gatcatgacg ctggtttcgg tggtgccgct
     9661 cgtcggcgtc gccgccgcgt cgatcctggt gccggccttt tccgtgggct tcatggccgc
     9721 ggcgcgggcg gcggcaagcc gcggaccggt ggagctcgcg cagcttttcg acggttttcg
     9781 ccaccatctc aggagccaga tggtgctcgg ggtggtctac ctcgcctgtc tcgcgttgct
     9841 gctcgccgcg accgcgctgg ccgacgaggg tgcgcttgcg acgtggatgc tcgccgggcg
     9901 caagccggag gacgaagtcc tgcagtccga ggagtttctt actgcgctcg tcgcggcagc
     9961 ggcgctttat gcgccggtgc tgatgatgtt ctggttcgcg ccgctgcttg ccgcctggca
    10021 cggggccggt cccgcgaagg cggttttctt cagcttcttc gcctgcctca tgaactggcg
    10081 tgccttcctc gcgtacggcg ccgtcaccgc gcttctcacc ctggcgctgc cgctggcagt
    10141 gctcggcatc gtgacgctcg cttcgctgaa agttgccccg atggcgctgg tgtttccgtt
    10201 gctcatcgtg ctgttgccga ccttgttcgc cagcttctac gccagctata gggacgtatt
    10261 cggcgctgaa tgaatcgcat ccgcgaaatt ccctacaact acacgtcgtt ctccgaccgt
    10321 gagatcacgc tgcgtcttct cggcccggac ggctgggcgc tgatcgccga gctgcgcgac
    10381 gagcgccgca ccggccgctc gtcgcgcatg ctctacgaag tgctgggaga gatctgggcc
    10441 atccagcgca atccctatct cgaggacgag ctggtcaacc ggccgcagcg ccgcgccgag
    10501 gcgctcggcg gcatgcgccg gcggctggcc gagatcgaag cgcgccgcgc cggaagtctc
    10561 aaggtggcgc ggctcctgga gctcacgcgc gcggcagtcg acggcttcga ggaagaattc
    10621 gccgtcacgc gcgcgctgcg cgccaaggcg atgcggcgcc tggcgcgcgt cacacgccgc
    10681 gacaacatcc tgttcgacgg cttcgcgcgc gtctcgcacg tcaccgacgc taccgactgg
    10741 cgcgtcgagg tgccgttcgt ggtgctcacc ccggacagcg aagaggaaat ccaaccgctg
    10801 gtccgggagc tgatcgccct cggcctcacc atcatcccgc gcggcggcgg cacgggctac
    10861 accggcggcg tcatcccgct cacgcgccgc tcggcggtga tcaacaccga gaagctcgaa
    10921 agcctcggac cgatcgagca cgccgagctt cccggcgtgg cgcgcccgca gcccacgatc
    10981 cgcgcatcgg cgggagtggt gacccggcgc gtgatggacc tcgccgagga gggcgggctg
    11041 gtattcgcgg tcgatccgac ctccgcggat gcttcgtgca tcggcggcaa cgtggcgatg
    11101 aacgccgggg gcaagaaggc cgtgctctgg ggcaccgcga tcgacaacct ggtctggtgg
    11161 cgcatggtga cgccgcaggg cgagtggctc gaggtcgagc gactcgagca caacctcggc
    11221 aagatccacg acgcgccgaa ggtgcgcttc ggattgaagt atttccaggg aaagaagctg
    11281 aagcaggagc gcgttctcga gatccccggg tcgcacttcc gcaaggcggg cctcggcaag
    11341 gacgtgagcg acaagttcct cgccgggctg ccgggggtcc agaaggaggg caccgacggc
    11401 atcatcgtcg cggcgcgctt tctcctgcac cgcatgccgg cgcacacgcg cacggtgtgc
    11461 ctggagttct tcgggctggc gcgcgatgcc gttccggcca ttgtggaagt aaaaaactac
    11521 attgaaaaat cgaaagcggt cttgttggcc ggcctggagc acctcgacga gcgctacgtc
    11581 aaggcggtcg gctacgccac caagtcgcgg cgcagccagc gcccgcgcat ggtgctgatc
    11641 ggggacctcg tcggcgacga cgtcgacgcg gtcgccgcgg cggcgtccgg ggtggtgcgc
    11701 atcgcgaacg cccgcggcgg cgaagggttc atcgcggtct cgcccgaggc gcgcaagcgc
    11761 ttctggctcg accgcgcgcg caccgcggcg atcgccaagc acaccaacgc cttcaagatc
    11821 aacgaggacg tggtgatccc gctggagaag ctcggcgagt acaccgacgg cgtcgagcgc
    11881 atcaacatcg agttctcggt gcgcaacaag ctcgagctgc tggacgccct ggaagagtat
    11941 ttctccggcg aattggcggt cgaggatagg gacctgctcg gcgagcggcc cggccgcgcg
    12001 ctcgagctgc tgcggcgggt gcgcggccgc tggcaggcga tcgcctcgga catggaccac
    12061 tacttcgacg agctccagtc ctaccgccag cgcctgtcat ggaagaccga ggtgcgcgac
    12121 gagctgcgcc acatcttcga cggcgtcgcc ttccgccgga ttctcgaagg gactgaggag
    12181 gctcacaaga ggatcctccg gggacgtgtc ttcgtcgcac tgcatatgca cgccggcgac
    12241 ggcaacgtgc acaccaacat cccggtcaac tccgacgact accagatgct gcagcgtgca
    12301 tccgacgcgg tggcgcgaat catgcgcctc gcgcgctcgc tcggcggcgt gatctccggc
    12361 gagcacggca tcggcatcac caagctcgaa ttcctcgagc ccgccgagat cgaggcgttt
    12421 cgcgcgtaca agcaggcggt cgatcccgag ggccgcttca acgccggcaa gctcctcgcc
    12481 ggcggcgacc tggccgacgc ctatacgccg agcttctcgc tgatcggcgc cgagtcgctg
    12541 atcctcgacg agaccgacct cgggcgcgtc gccggcgcga tcaaggactg cctgcgctgc
    12601 ggcaagtgca agccgccctg cgcgacgcat gtcccgcgcg ccaacctgct gtattcgccg
    12661 cgcaacaaga tcctcgccac ctcgctcctg atcgaggcga tcctctacga ggagcagacg
    12721 cggcgcggtg tcgcgctcgc gcactacgac gagttcggcg atgtcgccga tcactgcacc
    12781 gtgtgccaca agtgcgagcc ggcgtgcccg gtggacatcg acttcggcga cgtttcgatc
    12841 atcatgcgca actacctgcg ccgcgccggc aagcgccgct tcaacccggg aaccaaggca
    12901 tcgatgctgt tcctcgggct gaccgacccg cgcgcggtga agatggcgcg caccgtgatg
    12961 atcgattggg gttaccgggc ccaaagggcc gctttttcgc ttttcaagac tttcggagtt
    13021 gtgagaaagc agacggcggc gccgccgctc accacgggcc agaagccgct gcgcgcgcag
    13081 gtcgtccact tcatcaacaa gccgatgccg gggcggctgc cgaagaagac cgcgcgggcg
    13141 ctgctggaca tcgaggacgc gaaagtggtg ccgatcatcc gcgatccggc gaaggtgaac
    13201 gaagactcgg acgcggtgtt ctacttcccg ggctgcggct cggagcgcct gttctctcag
    13261 gtggggctcg ccacgcaggc catgctctac gaggtgggca cgcaatgcgt gctgccgccg
    13321 ggatacctgt gctgcggcta cccgcagacc gcagccggcg agtcggagaa gggcgagcgc
    13381 atcatcgtgc agaaccgcgt gctcttccac cgtctcgcca ccacgctcaa ctatctcgat
    13441 atccgcaccg tgatcgtctc gtgcggcacc tgcctcgacc agctcgagaa gtacgagttc
    13501 ggtgcgatct tccccggcgc ccggctcctc gacatccacg aatacctgct cgagaaaggc
    13561 gtgaaggcga acgatgtccc cggggtcaaa tacctgtatc acgatccctg ccacacgccg
    13621 atcaagaccg ccaagccgct cagcgtggtg aacggcctga tcggagaggc tcgcctttcc
    13681 gagcgctgct gcggcgagtc gggcacgctc gcgatgacgc ggcccgacat ctcgacccag
    13741 gttcgcttca gcaaggaaga gtcgctcagg aagggcattg ccgatctcgg cggcgacgct
    13801 tccccggtca agattctgac ctcctgcccc tcctgccttc agggcctcgc gcgcttcgag
    13861 gatgacacgg gcgcgcaggc ggactacatt gtggtcgagc tggcgcggcg gttgctcggc
    13921 gagaactggc tgcccgagtt cgtcgccaag gcaaacgccg gcggcatcga aagggttctg
    13981 ctgtgatcga gcgcgtaccc accggcatca cgctgcacca gaagtcgcgc gtgctggaga
    14041 tcgcgttcgc cgacgggcgc acgtttcggc tgccgtacga gttcctgcgg gtgtattcgc
    14101 cctcggccga ggtgcgcggc cacggccccg gccaggaggt gctgcagatc ggcaagcgca
    14161 acgtcgagat ccgctcgctc gagccggtgg gctcgtacgc ggtgcagccg cagttttccg
    14221 acgggcacag caccggcatc tattcgtggg actatctgta cgagctcggc gagagccagg
    14281 agagcctctg gtcccaatac ctggagaggc tcaaggcggc caatgcaagc cgggaataaa
    14341 gccgacaccg taaagcgcgt cttcgactcg gtggccgagc gctacgacgt catgaacgac
    14401 ctgatgtcgc tcggcctgca ccgcgcgtgg aaggcgttcg cggtttccat cgcgcggccg
    14461 aagccgggcg agcgcatgct cgacattgcc accggcagcg gcgacctggc gctcgcgctc
    14521 tcgcgcaggg tgctgcccgg cggcgaggtg tggatcaccg acatcaaccg gcggatgctc
    14581 gagcgcggcc gcgaccggct gctcgatcgc ggtcttgcgc tgcccgcggt gcagtgcgac
    14641 gccgagcggc tgccgtttcc gtcggcgcat ttcgattgcg tcaccgtcgg cttcgggctg
    14701 cgcaacatga cgcgaaaaga cgccgcgctc gccgagatgg cgcgcgtgct gaagcctggc
    14761 gggcgcctgg tggtgctcga attctccaaa gtctggaagc cgctcgagcg cgcctacgat
    14821 tggtactctt tcaaggtgtt accgtggctc ggggacaggg tcgcgggcga cgccgacgcc
    14881 taccgctacc tcgccgagtc gatccgcctg caccccgacc agggcgcgct cgccgagatg
    14941 ctgcgcgccg cgggcctcgc ggacgtcgaa gtgtttaacc ttgccgcggg cgtggttgcg
    15001 gtccatagag gggtgcgttt ttgatagcat tgacggttcg gaggtgtgca tcatgagcaa
    15061 gtggctactt acagcggtaa tcatcggatt tggcggaatt ttcgtggcga cggaggtcga
    15121 agcccgccgt ctcggcggcg gccgcaacat gggcgcgcaa cgcaacgtga ccgctccgcc
    15181 ggcgcaaacg ccggccaagc agcaacaggc cgcgccggcg caacaacagc ccggccaagc
    15241 cactccggct gcggcgcgca cgccatgggc gggcatcctc ggcgggctcg cgctcggcgg
    15301 gatgctcggc tacctcttcg gcggcagcgg cctgatgggc ttcctgctcc tcgcgttgct
    15361 cgcgattgcc gcggtggtcg tggtacgcgc gttgatgcag cggcgccagg aaacctcgcg
    15421 accggtgcaa ttcgccggca tgcgcgaaac cgtgaatgtc acgcccgact ccggtggcgg
    15481 acagatgcca attgccaaga gcaatgttcc tgctggcttc gatgccggca gcttcctgcg
    15541 cgcggcgaag ctcaattacc tcaggctgca ggcggcgaac gacgctggac ggatggagga
    15601 catccgcgag ttcaccaccg acgagctctt cgaggcgcta aagagcgagc tgggcgagcg
    15661 cagccagacc gaggtcgccg ggctcgaggc cgagctgctt gaggttgcga ccgagggcaa
    15721 agagcattgg gctagcgtgc gcttctcagg ccaggtgcgc gaggcgcccg gcgccgcgcc
    15781 cgaggctttc gcggaagtct ggaacctcgt gaagcccgcc gacgggtcga gcggctggct
    15841 gctcgccggc atccagcaga tgcattgaac gagtcgctgt ccttccaaaa ggccaccccc
    15901 gcggtggcct tttgctttgt gttgaaccgg ctgctcgccg ccgagccttg ggcgcggctg
    15961 cgcctcgcgc ccttcgccgg cgagacggtc gagctgcgcg cgccgctcct tcccacgcta
    16021 cgcttgcgca ttctcgcggg gggaacagtg gaaccgggcg gcgctgaacc tgggttgacc
    16081 atgacgttga agcctgagct gctcgccgcg cttgcccgcg gcgaggagca cgcgatgcgc
    16141 tcggtcgacg tggaaggcga cccgaagcta gcggccgagg tcctggtcct cgcgcggcac
    16201 ctgcgctggg atgccgagga agacctgtcg cgcctggtgg gggacgtcgc ggcgcaccgt
    16261 ctcgcgcagg caggacgcgc gttcgcggcc tggcacctgg atgcggcgca gcgcctggcc
    16321 gcggcggtgg ccgactacgt caccgaggaa aagcagctcc tcgcgcgccg cgccgacctc
    16381 gtcaccctcg gcgattcggt cgcgcgcctg cgcgacgcgg ttgcaaggct cgacaagcgg
    16441 gtcgagcggc ttggctaggg ttacccggct cgcgcgcatc gtcgcgatcg gcctgcgctt
    16501 cgggctgcac gaattcgtcc cgcgcttttc gcgcaacctc cttgtaagag cgttcgccgg
    16561 gcacaagggc gagccgcgcg gtcaacgcct cagggaagcg ctcgagacgc tcgggccgat
    16621 ctacgtgaag ttcggccagg tgctctccac gcggcgggat ctcctgccgc tcgacatcgc
    16681 cgacgagctc gcccggctgc aggaccgcgt gccgcctttt ggctccgatc tcgcggtggc
    16741 ggaggtggag agatcgctgg gcaagccgct cgacgttttg ttctcgagtt ttgaaagaac
    16801 cccagtggca agcgcttcca tcgcccaggt ccatctcgcc gtcctgcacg acggcgccga
    16861 ggtcgcggtg aaggtgttgc ggcccggggt cgaggcggcg atcgcgcgcg acctcgagct
    16921 gctcgagacc ggcgcgggcc tcgccgagcg gctcctggtc gacggccggc gcctgaagcc
    16981 gcgcgaggta gtcgccgagt ttgcgcgcca cctggacgac gagctcgacc tcatgcgcga
    17041 ggcagcgaat gccagccagc tcggacgcaa tttcgccggc tcgccgctgc tcctcgtgcc
    17101 cgcggtgcac tgggatctct gcgcgcagcg cgtgatggtg atggagcgca tgcatggaac
    17161 accgatctct caagtcgagg tactaagaca aaagggaatt gacattcccg ccttggcccg
    17221 agccggggtc gagatcttct tcacccaggt gttccgccac gggttcttcc acgccgacat
    17281 gcatccgggc aacatcctcg tggcgacgga ggaggaaatg cgtgggcgct atatagccct
    17341 cgacttcggc atcatgggca cgctcaccga ggtcgacaag aactacctcg cccagaactt
    17401 tctcgcgttc ttcaaccgcg actaccggcg cgtggcgcag gcgcatctcg acgccggctg
    17461 ggtgccggcg ggcacgcgcg tcgacgagtt cgaggcggcg atccgcgcgg tgtgcgagcc
    17521 gatcttcgcg cgcccgctcc gggagattta tttcggaaag ctgctcctgc gcctgttcca
    17581 gacctcgcgg cgcttcaacg tcgaggtgca gccccagctc gtcatgctgc agaagacgct
    17641 gctcaacatc gagggcctcg ggcgcgagct cgacccggac ctcgacctgt ggcagaccgc
    17701 gaagcccttc ctcgagcgct ggatgcgcga gcaggtcggc tggcgcggcc tcgtccgcac
    17761 catccgccac gaggcgccgt tctgggccgc caccctgccg cagcttccgc gcctggtgca
    17821 tcgcgcgctc gccgaagacc gcctcggcgc gctgcatacc gcggtcgagc gcttggccga
    17881 cgagcacgca cggcgcaacc ggttgctctc cgtggtactc gtcgcgcttg tggtctggct
    17941 ggcggcgacg ctcgccacgc tgttcctcta gtcattcaac ggatggcgca ggacagctca
    18001 cgccccgagt tctgggacac gcgcttccgc gatggcgtca ccccgtggga cgcggggggc
    18061 gtgccgcctg ccttgaagaa ttggatcggg agacgcagcg gccgcctgcg tgtgctcatc
    18121 cccggcgccg ggaccggcta cgaggtgcgg cttttcgccg ggcatgggca cgacgtgctg
    18181 gccatcgact tctccgacgc ggccatcgag gccgcgaagc gcgagctggg ttcgctctcc
    18241 ggccgggtga ggaaggccga cttcttcggg ctgcaagatg gcccgttcga tgttgtctat
    18301 gaacgcgcgt tcctgtgtgc cctgccgcgc acgctgtggt ccgagtgggc gcggcggatg
    18361 gccgagctcg tgagtccggg gggcgtgctc gccggcttct tctacctcga cgacaaccag
    18421 cgcggcccgc cgttcggggt ttcgcaagag ggattgaaag accttctggg gaagacgttt
    18481 gagatgacgg aaagccgggt gctcccgccc gagcagtccc tgccggtatt ccaggggaag
    18541 gaaatttggc aggtttggac gcggcggcta tgattgcccg cgcgtgctga tcggctcggt
    18601 cgccctgtat ctcgccctca ccatcgccat cgggctgtgg gcggcgaagc gcgtgcacaa
    18661 cgccaaggac tacgtggtcg cgggccgcag cctgccgctt tacatgagcg cggccacggt
    18721 gttcgctacc tggttcggcg ctgagacggt gctctcggtc tcggcgacct tcgccaagga
    18781 cggcctgggc ggcatcgtcg ccgacccgtt cggcgcttcg ttctgcctgc tgttcgtggc
    18841 gatcttcttc gcgcgggcgt tttaccgcat ggatctgctg accatcggcg acttctaccg
    18901 caagcgctac ggcaagacgg tcgaagtgat caccagcgtc gcgatcaccg cttcgtatct
    18961 cggctggacc tcggcacagc tcaccgcgct cggcctggtg ttctcggtgc tgtccggcgg
    19021 cgccatcagc ctgagcaccg gcatcctcat cggcgccggg atcgtggtct tctacacgat
    19081 cttcggcggc atgtggtcgg tcgcgctcac cgatcttttc cagtcggtgg tgatcctggt
    19141 cggcctggtg gccgtggcgt ggctggtggg cgacatggcg ggcggcgcgg gcaaggtcat
    19201 cgccgccgcg tcggaagccg gcaagttcga gttctggccg aagggcggca ccaaggagtg
    19261 gctctggttc ctcgccgcct ggatgaccct cgccatcggc tcgatccccc agcaggacgt
    19321 gttccagcgc gtcactgcgg ccaaggacga gaagaccgcg gtgcgcggca cgctgatcgg
    19381 cggcttcgtg tacttctgct tcgccttcgt cccgattttc atcgcctatg ccgcgctggt
    19441 gatcgacccg ggctatgaaa agctcttcgc cggcgaggac gcgcgcgaga tccagcgcat
    19501 cctgcccgac ctgatcctca accgcacgcc ggtctgggcg caggtgctgt tcttcggcgc
    19561 gttgctctcg gcgatcctgt cgacggcgag cggcgcgcta attgccccga cgtccctctt
    19621 cactgagaac gtgatccggc cgttcttccg cggcatgagc gaccgccagt tcatgctcct
    19681 gcttcgcgtg atcctggtga ccttcacgct cgcggcgctg ctcttcgcgc tcaactccaa
    19741 gagcacgatg tacgagatgg tgcagaacgc ctacaaggtg accctggtgt catgcatcgt
    19801 gccgctcgcg gcgggcatct tctggaagcg cgccaatgtc ccgggagcga tcctatcggt
    19861 ggtctttggc ctggcctcct ggacgattgc ggaatgggtc gcggcggacg cgacggtgcc
    19921 gccgcagctc gtgggcctgg gcttctcgct cgtcgggatg gtgctcggct cgctcgtgcc
    19981 gagcgcagcg gtccagccgg cggcccacca ccacggcaag cactgagctc cccgcagcgt
    20041 caggcccgac gagcggctcg gcgctcgcgc gcatcctgct ccttgtgtat gccctgctcg
    20101 tcgtttacgc cacgctttac ccgcttgccg ggt
//
LOCUS       DATBSZ010000241        13695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1001373, whole genome shotgun sequence.
ACCESSION   DATBSZ010000241 DATBSZ010000000
VERSION     DATBSZ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13695
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001373"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VIG70_15240"
     CDS             complement(<1..512)
                     /locus_tag="VIG70_15240"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8251954.1"
                     /translation="MPCDILVRNATLIDGNGAPARRADIAISDGRIHSVEKAVSVNPR
                     KTIDASGLVVAPGFIDPHTHYDAQICWDGATTPSSWHGVTTVVMGNCGVGIAPCRPAA
                     REIAMRDLVNVEAIPFEVLDAGITWDWETFPQYMDAAAQRKPALNLAFLAPLTPFRHY
                     VMGEASMERAA"
     gene            547..978
                     /locus_tag="VIG70_15245"
     CDS             547..978
                     /locus_tag="VIG70_15245"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8251955.1"
                     /translation="MPGQTGGCLCGAIRYTIDAPIAGLRACHCQNCQKSSGTGGTVNA
                     VVPTDRFRITQGSTRKYDDSATRSGRILSRHFCEKCGSPIYSHRNPNPGFVVVRAGTL
                     DDKSALKIAGNIWTSTAASWAHIDPSTECHPENMPPPPPKA"
     gene            complement(997..1164)
                     /locus_tag="VIG70_15250"
     CDS             complement(997..1164)
                     /locus_tag="VIG70_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251956.1"
                     /translation="MSKASQARKLKAQKNLKKARVQLARFKARARAEMARHARAFKLA
                     AKRYKKAVKQG"
     gene            complement(1216..2193)
                     /locus_tag="VIG70_15255"
     CDS             complement(1216..2193)
                     /locus_tag="VIG70_15255"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251957.1"
                     /translation="MRLASILAALVLAAGTAQAQPLKKVVLFGQPSVNNDAIWMALEY
                     GYFKEQGLDVEYRVFPSGTTAFQTFRTGQGDIVMTGDLPSVQYFFNNPGQYQTFAAIE
                     RDAKGYVVIARKDIRKPADLAGKIVATRVGSTGSWFVSEYLAKNKVDPKTVSVKNLDT
                     QLLPAALCKGDISAFFIWQPIGSRTLEICPNDAHQLSDATGYVQGYLVAGARPEFLNT
                     PDGADKVTRFLRAIMKGRAKAESDFKAVAAFAAKTYSLSEKATRDQWDTNIRPIAIDK
                     VYYDDFCSLSKWARGAGLTTEPLDFTKLTWPNGLRAIDPKLALAPPPAC"
     gene            complement(2205..2963)
                     /locus_tag="VIG70_15260"
     CDS             complement(2205..2963)
                     /locus_tag="VIG70_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8251958.1"
                     /translation="MRAAAFFVGSIVIAFAGWQFLSTFVFNPFLIPPPLVVLETMIPM
                     VKSGEIFRHVAISLARVGIGFVTGCAAGIVLGVILGRIKLVNDLLDPIIELLRYLSPT
                     AMIPIAVIWFGIGETSKYFLIFWGTFFIVLINTTAGVMRAPIIRQRAAECLGASPLQI
                     FLLVVIPSAAPYIVTGMRIAMASSFMSIIPAEILAADSGIGYLLQHSSILLQTNRIFV
                     ALLTICVIGFIFDRLFRMAVDRMLSRYMSYIALT"
     gene            complement(2960..3703)
                     /locus_tag="VIG70_15265"
     CDS             complement(2960..3703)
                     /locus_tag="VIG70_15265"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251959.1"
                     /translation="MLAVRNVSKVFGAEPERYVALEGIDLSVSEGELVCLLGPSGCGK
                     STLLNILAGFEQASGGTVQFDGAPVRGAGKDRVMFFQDAGSALLPWYSAEENVRFALR
                     VRKLPKEQWPAIIDKYLAMVGLAEHRRKFPAQLSGGMRQRLQIARALAVEPKVLLMDE
                     PFGALDALTRRRMHGFLLDIWQRTGKTIVFVTHDIAEAVTLADRICLMSVGPGSRITE
                     VIDVGLPRPRDLGDPAAGRLFKRVEGLLM"
     gene            3736..4995
                     /locus_tag="VIG70_15270"
     CDS             3736..4995
                     /locus_tag="VIG70_15270"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8251960.1"
                     /translation="MANIEMRGVENLAGTAVPYAYAVKAGPWLFLTGHEAFDFAAGPA
                     PEVEGPAGFPSFGAPRLKREAEYILKRMRKTLAEFGSDFRHSVRVDQYYPVFEAVRAY
                     QLARHGEFGDYIPPSTSVLMKRLFSASSHISMSMIAVVPGKDYEIGKLYPKEVPVPVG
                     SFFVPAITCNDFVFVAGQMASDENALDPSVRLPKEVRNWGGPNAVRRQTEFIIKKRLE
                     PALKAGGASWKDILKAQIYVPTAADIPDALDVWQQHVGAHPCALTAVPTHGFGFIDGI
                     VEINLLALRDGAKRKKEVLKVDLPAMSMYGAVVRAGELVFPSGFMPVGFASSDPFDSL
                     ARASQVQALAALGFADKVCRAAGTTLQNVVRAQWFMADATQFPGVALAWMSRQGKQPH
                     PFVCVQVPAPLPAPGAALTADFWIYAP"
     gene            complement(4992..6110)
                     /locus_tag="VIG70_15275"
     CDS             complement(4992..6110)
                     /locus_tag="VIG70_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8251961.1"
                     /translation="MRTPPRILVADDTPANVRMLETRLSHDGYEVVTARDGEEALAVA
                     RATRPDLILLDIMMPKVDGIAVCRALKGDPAIPFTPIIMVTALADAKDVVAGFEAGGD
                     DYLTKPFDAQSLSARVKSMLRIKQLHDTVQEQAAELERWNATLEERVRQQLAELERVG
                     RLKRFFSPQLAELIVQGGAGDPLQSHRREITVVFLDLRGFTAFAEVAEPEEVMSVLRE
                     YHARMGETILAHEGTLERFTGDGMMIFFNDPVPVPNPAERALRMTLEMRARLEELAAR
                     WRKSGYELHAGIGIAQGYATIGTIGFEGRWDYSAIGTVSNLAARLCAEAQGGQILISQ
                     RVQAAIAELADTEPAGELNLKGFQRPVTAFTVLRLKRA"
     gene            6140..7750
                     /locus_tag="VIG70_15280"
     CDS             6140..7750
                     /locus_tag="VIG70_15280"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8251962.1"
                     /translation="MRLARTDPLAAFLLRTPLGALVRWVAGVRSTVQRKLLAAFLGIA
                     FLVLAMGAFSVYTMRGMSRQSELLDQAHQRLDWSQQIHHSLAMQMHLTAMALLVRDEK
                     ALASILRENNRFNEALAHLESAVEESEKLVIQKIRTGQEEAMTLVADIANLVRDGKID
                     AATRLHGTREDPLYRSIDALVAEVVQKENARMVGLRESLRATNQRSLLVIGSAAAIAV
                     LLALAGGFVISWALIQPVRAAHGFLDELSAGRFGGSVTVQNRDEFGALAEQLTATSQE
                     LARLDEEQRRAARELGALNARLEQASKAKSEFLANMSHELRTPLNAILGFTEMLTDGL
                     YGPLPGEFGEPLADIQTNGKHLLRLINDVLDLSKIEAGRMELALSEYSASDVVTSVRA
                     SLRSLAEEKGLHFKVAIDDGLPEIVYGDPKRVTQCLFNLAGNALKFTKQGGVTIRAAR
                     DGDSVRYSVTDTGIGIPEDQLGAVFAEFRQVDSTITREFGGTGLGLSITKKFVEMHGG
                     RIWVESRLNEGSTFHFTIPIKAQVTSTV"
     gene            7747..8115
                     /locus_tag="VIG70_15285"
     CDS             7747..8115
                     /locus_tag="VIG70_15285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8251963.1"
                     /translation="MKTILYVEDNEFNRKIVRQLLAKTKYKLREAHDGESAVKDALGD
                     PPDLILMDVQLPKMSGLDATRQLRADKRTAAIPIVVITSYALSGDAEKARDAGATAYL
                     AKPYSPRDLLAKIRELVPEA"
     gene            8129..8551
                     /locus_tag="VIG70_15290"
     CDS             8129..8551
                     /locus_tag="VIG70_15290"
                     /inference="COORDINATES: protein motif:HMM:NF013996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HEY8251964.1"
                     /translation="MAKLTRRIAVIAAAGAFSLPAAAGQEEQFTPEFAAVRSSFDEKH
                     TPKLTAPDSVKRGQWFDVSVNVGAGGVHPSLSEHHVRYIALYKDTAEIARVYLHPVFS
                     APRVTFTIALDSSGVLRALAEPTHSAAWEASKKIKVAP"
     gene            complement(8535..9230)
                     /locus_tag="VIG70_15295"
     CDS             complement(8535..9230)
                     /locus_tag="VIG70_15295"
                     /inference="COORDINATES: protein motif:HMM:NF026037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY8251965.1"
                     /translation="MRAKVLLFAGCLASSGWALGGDLTGTVLFSGALPERPKVPVTID
                     QYICGKEKDADDLVLGPKRGVQNAVVWIENPGAGAKWPAQPDKVEMDQKGCVFVPRVL
                     VVPVGGTVDFLNSDRLLHNLRSVSKQNPEFNRTQPHNRTIPITFTKPELVTIACDLHS
                     WMKSWVVVAEHPFYAVTDAAGAYRIANVPPGKYRVRMWQEALGQTTREVTVGAQGGAT
                     DFDIKTLAPKARP"
     gene            9328..9618
                     /locus_tag="VIG70_15300"
     CDS             9328..9618
                     /locus_tag="VIG70_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY8251966.1"
                     /translation="MGKGYIVVTYRKIKDQAKWEAYAKLAVLAVQKSGGRFIVRATPT
                     RVHELGMKERVVIVEFDNVHKAMSAHDTPGYQEALKALGDGAERDFRIVEGV"
     gene            9673..10167
                     /locus_tag="VIG70_15305"
     CDS             9673..10167
                     /locus_tag="VIG70_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8251967.1"
                     /translation="MVSAMIARAIPKTGPRRCRLLYVEDHPDSVALVETLLAGRKDLL
                     LLRAADANLGIKLARAQRPEAILIDVDLAGMGALEFMKVLRADPATEATPILALGANA
                     APEAIVKALEAGFFQYLAKPLRAEPFMEALVFALEFAAVERSEHNALMLRFGHPRTPF
                     KESR"
     gene            10164..10460
                     /gene="groES"
                     /locus_tag="VIG70_15310"
     CDS             10164..10460
                     /gene="groES"
                     /locus_tag="VIG70_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY8251968.1"
                     /translation="MKIRPLHDRVIVKRIDEEKKTASGIVIPDTAAEKPDQGEVTAVG
                     KGKRTDEGELVPLDVKVGDRVLFGKYSGQTVRVKGDELLVMREEDIMGIVEGQQ"
     gene            10457..12103
                     /gene="groL"
                     /locus_tag="VIG70_15315"
     CDS             10457..12103
                     /gene="groL"
                     /locus_tag="VIG70_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430503.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY8251969.1"
                     /translation="MTIKDVRFHEDARTRMVRGLNILADAVKITLGPRGRNVVLDRSW
                     GSPTVTKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIVRE
                     GMKYVASGMNPMDLKRGIDLAVVTVVEELKKISKPCASSKEVAQIGSISANLDTEVGK
                     IIADAMEKVGKEGVITVEDGKSLHNELELVEGMQFDRGYLSPYFINNPEKQIAVLEDA
                     YVLLCDKKISSIRDMLPLLEQVAKAGKPLLVLAEEVEGEALATLVVNNLRGILKTLAV
                     KAPGFGDRRKAMLDDIAVLTGATVISEELGLQLEKTTLKDLGRVKKVESTKENTTLID
                     GAGDKKLIEGRIKQIRLQIEEQTSDYDKEKLQERLAKLAGGVALIKVGAATEIEMKEK
                     KARVEDALHATRAAVEEGVVPGGGVAYLRARARLKDLKGANRDQDGGIQIVLRALEEP
                     LRQIVANSGAEPSVVLNKVLEGKDSFGYNAQTAEFGDLVQQGVIDPTKVVRFALQNAA
                     SVAGLVLTTAAMVAERVEEDKGGGHQGGMGGMGGMGGMGM"
     gene            complement(12130..12762)
                     /locus_tag="VIG70_15320"
     CDS             complement(12130..12762)
                     /locus_tag="VIG70_15320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003274158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8251970.1"
                     /translation="MKLKAEELARIAKVTLEHYEQGAEEFWRGTRDHDVSQNIGELLR
                     HIEGAPPFTILDLGCGPGRDLKALSALGHLAVGLEGAPTFAAMARAHSGCEVLEQDFL
                     GLDLPGGRFDGVFANAVLFHVPGEALPRVLRELRAALKPRGVLFSSNPHGRNEEGWNR
                     GRYGVYHDLGSWRRYLTQAGFAELGHYYRPPGLPREQQPWLASLWRNAAA"
     gene            12798..13643
                     /gene="ylqF"
                     /locus_tag="VIG70_15325"
     CDS             12798..13643
                     /gene="ylqF"
                     /locus_tag="VIG70_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEY8251971.1"
                     /translation="MIAWFPGHMASARKKAAQTMGRIDVVVELLDARLPGASANPLIE
                     RLRRDKQRPCLKILNKADLADPDATAAWLRHYGNEKLVRAVALSCKRPGDVAKVPRLA
                     AALVPHRDSALKPVRMMVMGVPNVGKSTLINALLKRKVAKTGDEPAVTREQSRFELDE
                     RNELIDTPGLMAPVIKHAADGLMLAASHLIGPESYQAEEVAAFLAGILLERYPAQLRT
                     RYGFDTQGMDGDGLIAAIAAKRGYLGKGGTPHLEKAARALLQDYRSGLLGRMTLETPS
                     SRLIA"
BASE COUNT         2359 a         4701 c         4554 g         2081 t
ORIGIN      
        1 gccgcccgtt ccatcgacgc ttcgcccatc acgtagtgcc tgaagggcgt gagcggcgcg
       61 aggaacgcga ggttgagcgc gggcttgcgc tgcgccgccg cgtccatgta ctgcgggaac
      121 gtctcccagt cccacgtgat cccggcgtcg aggacctcga acgggatcgc ctccacgttc
      181 accaggtccc gcatcgcgat ctcgcgcgca gccggcctgc agggcgcgat ccccaccccg
      241 cagttcccca tcactaccgt cgtcacgccg tgccagctcg acggcgtggt cgcgccatcc
      301 cagcagatct gcgcgtcgta gtgggtgtgc gggtcgatga agcccggcgc gacgaccagc
      361 cccgaggcat cgatggtttt tcgggggttt acggaaacgg ctttttcaac ggaatggatt
      421 cgcccgtccg aaatcgcgat atccgcccgc cgcgccggcg cgccgttgcc gtcgatgagc
      481 gttgcgttcc ggaccaggat gtcgcagggc atcctggaat cataccgggc gctagaatta
      541 ggccccatgc ccggccaaac cggcggctgc ctctgcggcg ccatccgcta caccatcgac
      601 gcgcccatcg ccgggctgcg cgcctgccac tgccagaact gccagaagtc ctcgggcacg
      661 ggcggcaccg tcaacgcggt cgtgccgacc gacaggttca ggatcaccca gggctcgacg
      721 cgcaagtacg acgactccgc cacgcgcagc ggccgcatcc tgagccgcca tttctgcgag
      781 aagtgcggct cgccgatcta cagccaccgc aaccccaatc ccggcttcgt cgtggtgcgc
      841 gccggcaccc tcgacgacaa gagcgcgctc aagatcgccg gcaacatctg gacctccacc
      901 gccgcctcgt gggcgcacat cgatccttcg accgagtgcc accccgagaa catgccgccc
      961 ccgccgccga aggcctgagc ggctcagcgc gggcgcttaa ccctgcttca ccgccttctt
     1021 gtagcgcttc gcggcgagct tgaacgcgcg cgcgtgccgc gccatctcgg ccctcgcccg
     1081 ggccttgaac ctggcgagct ggaccctggc tttcttcagg ttcttctgcg ccttgagctt
     1141 cctcgcttgg gatgccttgg acatgagcct tctccctcga cggttggaca acgtggccgc
     1201 atcctagccc aactcctagc aggctggcgg cggcgccagg gcgagcttcg gatcgatcgc
     1261 gcgcaacccg ttcggccagg tgagcttggt gaagtcgagc ggctcggtgg tgagcccggc
     1321 gccgcgcgcc cacttggaga ggctgcagaa gtcgtcgtag tacaccttgt cgattgcgat
     1381 cgggcggatg ttggtgtccc actggtcgcg cgtcgccttc tcgctcaggc tgtaggtctt
     1441 ggcggcgaac gccgcgacgg ccttgaagtc gctttcggcc ttggcgcggc ctttcatgat
     1501 ggcgcgcagg aagcgcgtca ccttgtccgc gccgtcgggg gtgttgagga actcggggcg
     1561 cgcgcccgcc accaggtagc cctggacata gccggtcgcg tccgaaagct ggtgggcgtc
     1621 gttcgggcag atctcgagcg tgcgcgagcc gatcggctgc cagatgaaga aagccgagat
     1681 gtcgcccttg cacagcgccg ccggcagcag ctgcgtgtcg aggttcttga ccgacacggt
     1741 cttgggatcc accttgttct tcgccaggta ctccgagacg aaccacgacc cggtcgagcc
     1801 gacgcgcgtg gcgacgatct tgccggcgag gtccgcgggc ttccgaatgt ccttgcgcgc
     1861 gatcaccacg tagcccttgg cgtcgcgctc gatcgcggcg aaggtctggt actgccccgg
     1921 gttgttgaag aagtactgca cgctcggcag atcgccggtc atcacgatgt cgccctggcc
     1981 ggtgcggaag gtctggaacg ccgtggtgcc cgacgggaac acgcggtact cgacgtcgag
     2041 gccctgctcc ttgaagtagc cgtactcgag cgccatccag atggcgtcgt tgttcaccga
     2101 aggctggccg aacaggacga ctttcttcag gggctgcgcc tgcgccgttc ccgccgccag
     2161 aaccagtgcc gccaagatgc ttgccagtct catgaggcct cctcctaggt cagcgcaata
     2221 tacgacatgt agcgcgagag catgcgatcg accgccatgc ggaacaagcg gtcgaagatg
     2281 aagccgatga cgcagatcgt cagcagcgcg acgaagatgc ggttggtctg gagcaggatc
     2341 gaggagtgct gcagcaggta gccgatgccc gaatccgccg ccaggatctc ggccgggatg
     2401 atgctcatga acgacgaggc catggcgatg cgcatcccgg tcacgatgta cggcgccgcc
     2461 gagggaatca ccaccagcag gaaaatctgc agcgggctgg cgcccaggca ctcggccgcg
     2521 cgctggcgga tgatcggcgc gcgcatcacc ccggcggtgg tgttgatcag cacgatgaag
     2581 aacgtgcccc agaaaatgag gaaatacttg gaggtctcgc cgatgccgaa ccagatcacc
     2641 gcgatcggga tcatcgccgt gggcgaaaga tagcgcagca gctcgatgat gggatcgagc
     2701 aggtcgttga cgagctttat ccttcccaaa atgaccccca acacgatgcc cgcggcgcat
     2761 cccgtcacga agccgatgcc gacgcgcgcg aggctgatcg ccacgtgccg aaagatttcc
     2821 ccgctcttca ccatcgggat catggtctcg agcaccacca gcggcggcgg aatcaggaag
     2881 gggttgaaga cgaaggtcga aaggaattgc cacccggcaa aggcgatgac gatggacccg
     2941 acgaaaaacg ccgccgccct cacatcaaca gcccttccac gcgcttgaag agccgccccg
     3001 ccgccggatc gccgaggtcg cgcggccgcg gcaggccgac gtcgatcact tcggtgatgc
     3061 gcgaccccgg cccgaccgac atgagacaga tccggtcggc gagggtcacc gcctcggcga
     3121 tgtcgtgggt gacgaagacg atggtcttgc cggtgcgctg ccagatgtcg agcaggaagc
     3181 cgtgcatcct gcgccgcgtc agcgcgtcca gcgcgccgaa cggctcgtcc atgaggagca
     3241 ccttcggctc gaccgccagc gcccgcgcga tctgcaggcg ctggcgcatg ccgcccgaga
     3301 gctgggccgg gaacttgcgg cgatgctcgg cgaggccgac catcgccagg tacttgtcga
     3361 tgatggccgg ccattgctct ttcggcagct tgcgcacgcg cagcgcgaag cgcacgttct
     3421 cctcggcgga gtaccacggc aggagcgccg agcccgcgtc ctggaagaac atcacgcggt
     3481 ccttgcccgc gccgcgcacc ggcgcgccgt cgaactgcac cgtgccgccg ctcgcctgct
     3541 cgaagcccgc gaggatgttg aggagcgtgg acttgccgca gccggagggg ccgagcaggc
     3601 agacgagctc gccttccgac accgacaggt cgatgccctc gagcgcgacg tagcgctccg
     3661 gctccgcgcc gaagaccttg ctgacgtttc gcaccgcgag cacagcgcct cacccccctc
     3721 gtcgtatcct aacgcatggc caacatcgaa atgcgcggcg tggagaacct cgccggaacc
     3781 gccgtgccgt acgcctacgc agtgaaggcc ggcccgtggc tgttcctcac cgggcacgag
     3841 gcgttcgact tcgccgccgg ccccgcgccc gaggtcgaag ggcccgcggg ctttccttcg
     3901 ttcggcgcgc cgcgcctcaa gcgcgaagcc gaatacatcc tgaagcgcat gcggaaaacg
     3961 ctcgccgagt tcggctccga cttcaggcac agcgtgcgcg tcgaccagta ctacccggtg
     4021 ttcgaggcgg tgcgcgccta ccaactggcg aggcacggcg agttcggcga ctacatcccg
     4081 ccttccacct cggtgctgat gaagcgcctg ttcagcgcaa gctcgcacat ctccatgtcg
     4141 atgatcgcgg tcgttcccgg gaaggactac gagatcggca agctctaccc gaaggaagtg
     4201 ccggtgccgg tcggctcgtt cttcgtgccg gcgatcacct gcaacgactt cgtgttcgtc
     4261 gccggccaga tggcgagcga cgagaacgcg ctcgacccgt cggtgcgcct gccgaaggaa
     4321 gtgcgcaact ggggcggccc gaacgcggtg cgccgccaga ccgagttcat catcaagaag
     4381 cgcctcgagc ccgcgctcaa ggcgggcggc gcgtcatgga aggacatcct caaggcgcag
     4441 atctacgtcc cgaccgcggc cgacattccg gacgcgctcg acgtgtggca gcagcacgtc
     4501 ggcgcgcacc cgtgcgcgct caccgccgtg cccactcacg gcttcggctt catcgacggg
     4561 atcgtcgaga tcaacctcct cgccctgcgc gacggcgcga agcgcaagaa agaggtcctg
     4621 aaggtcgacc tgccggcgat gtcgatgtat ggcgcggtcg tgcgcgccgg cgagctcgtg
     4681 ttcccgtcgg gtttcatgcc ggtcggcttt gcttcttcgg atcctttcga cagccttgcg
     4741 cgcgcctcgc aggtccaggc cctcgccgcc ctcggcttcg ccgacaaggt ctgccgcgcc
     4801 gccggcacca cgctccagaa cgtcgtgcgc gcgcagtggt tcatggccga cgccacccag
     4861 ttcccgggcg tcgcgctcgc ctggatgagc cggcagggaa agcagcccca tcccttcgtc
     4921 tgcgtccagg tccccgcccc gctccccgcc ccgggcgcgg cgctcaccgc cgacttctgg
     4981 atctacgccc cttaggcgcg cttcagccgt agcaccgtga aggccgtcac cgggcgttgg
     5041 aaacccttga gattcagctc gccggccggc tcggtgtcgg cgagctcggc gatcgccgcc
     5101 tggacgcgct ggctgatcag gatctggccg ccctgcgcct cggcgcacag ccgcgcggcg
     5161 agattgctca cggtgccgat ggcgctgtag tcccagcgcc cctcgaagcc gatcgtgccg
     5221 atcgtggcgt agccctgggc gatgccgatg ccggcatgga gctcgtagcc gctcttgcgc
     5281 cagcgcgccg cgagctcctc gaggcgcgcg cgcatctcga gggtcatgcg cagcgcgcgc
     5341 tccgccgggt tgggcaccgg caccggatcg ttgaagaaga tcatcatgcc gtcgccggtg
     5401 aagcgctcga gcgtgccttc gtgcgccagg atcgtctcgc ccatgcgcgc gtggtactcg
     5461 cgcagcaccg acatcacctc ctcgggctcg gccacctcgg cgaacgcggt gaagccgcga
     5521 agatcgagga acaccaccgt gatctcgcgc cggtgcgatt gcagcgggtc gccggcgccg
     5581 ccctgcacga tcagctcggc gagctggggc gagaagaagc gcttcagccg cccgacgcgc
     5641 tcgagctcgg cgagctgctg gcgcacgcgc tcttccagcg tggcgttcca gcgctcgagc
     5701 tcggcggcct gctcctgcac cgtgtcgtgc agctgcttga tgcgcagcat cgacttgacg
     5761 cgcgccgaga gcgactgcgc gtcgaacggc ttggtcaggt agtcgtcgcc gccggcctcg
     5821 aagccggcga ccacgtcctt cgcgtcggcc agcgcggtga ccatgatgat cggcgtgaac
     5881 ggaatcgccg ggtcgccctt cagcgcgcgg cacaccgcga tgccgtcgac cttcggcatc
     5941 atgatgtcga gcaggatgag atcggggcgc gtcgcgcgcg ccacggcgag cgcctcttcg
     6001 ccgtcgcgcg cggtcactac ttcgtagccg tcgtgcgaga gccgcgtctc gagcatgcgg
     6061 acgttcgccg gcgtatcgtc cgcgaccagg atgcgaggcg gggtcctcaa gcgcgtggag
     6121 tatactcgcc gcgcttccaa tgcggctcgc tcgaactgat ccgctcgcgg cgtttctcct
     6181 gcgcactccg ctcggagccc tggttcgctg ggttgcgggc gtgcgctcga cggtacagcg
     6241 caagctcctc gcggccttcc tcgggatcgc gttcctcgtc ctcgccatgg gcgcgttcag
     6301 cgtgtacacc atgcgcggca tgtcgcggca gagcgagctc ctcgaccagg cgcaccagcg
     6361 cctcgactgg tcgcagcaga tccatcactc gctcgccatg cagatgcacc tcaccgcgat
     6421 ggcgctactc gtgcgcgacg agaaagcgct ggcgtcgatc ctgcgcgaga acaaccgctt
     6481 caacgaggcg cttgcccacc tggagtcggc ggtggaggag tccgagaagc tcgtgatcca
     6541 gaagatccgc accggccagg aagaggcgat gaccctggtc gcggacatcg cgaatctcgt
     6601 gcgcgacggg aagatcgacg cggcgacgcg gctgcacggc acccgcgagg acccgctcta
     6661 ccgctcgatc gacgcgctgg tcgccgaggt ggtgcagaag gagaatgcgc gcatggtcgg
     6721 cctgcgcgag agcctgcgcg ccaccaacca gcgctcgctc ctcgtcatcg ggagcgcggc
     6781 ggcgatcgcc gtgctgctcg cgctcgccgg cggcttcgtc atctcgtggg cgctgatcca
     6841 gccggtgcgc gccgcgcacg gctttctcga cgagctctcc gccgggcgct tcggcggctc
     6901 ggtcacggtg cagaaccgcg acgagttcgg cgcgctcgcc gagcagctca ccgccacctc
     6961 gcaggagctc gcccgcctcg acgaggagca gcggcgcgcc gcgcgcgagc tcggcgcgct
     7021 caacgccagg ctcgagcagg cgagcaaggc gaaatccgag ttcctcgcca acatgagcca
     7081 cgagctgcgc acgccgctga acgcgatcct cggcttcacc gagatgctca ccgacgggct
     7141 gtacgggccg ctgcccgggg aattcggcga gccgctcgcc gacatccaga ccaacggcaa
     7201 gcacctgctg cgcctgatca acgacgtgct cgacctttcc aagatcgagg ctggccgcat
     7261 ggagctcgcg ctgtccgagt actcggcaag cgacgtggtg acgagcgtgc gagcatcgct
     7321 ccgctccctc gccgaggaga agggcctgca cttcaaggtc gcgatcgacg atggcctgcc
     7381 cgagatcgtg tacggcgacc cgaagcgcgt cacccaatgc ctgttcaacc tcgccggcaa
     7441 cgcgctcaag ttcaccaagc agggcggcgt cacgatccgc gcggcgcgcg acggcgacag
     7501 cgtgcgctac agcgtcacgg acaccggcat cggcattccc gaggaccagc tcggcgcggt
     7561 gttcgccgag ttccgccagg tcgattccac catcacgcgc gagttcggcg gcacgggcct
     7621 cggcttgagc atcaccaaga agttcgtcga gatgcacggc gggcgcatct gggtcgagag
     7681 ccgcctgaac gagggctcga ccttccattt cacgatcccc atcaaggcgc aggtgacgag
     7741 cacggtatga agaccatcct gtacgtcgag gacaacgagt tcaaccgcaa gatcgtgcgc
     7801 cagctcctgg cgaagacgaa gtacaagctg cgcgaggcgc acgacggcga aagcgcggtc
     7861 aaggacgcat tgggggatcc gcccgacctg atcctgatgg acgtccagct cccgaagatg
     7921 tcgggcctcg acgcgacgcg ccagctccgc gccgacaagc gcaccgccgc cattcccatc
     7981 gtcgtcatca cgtcgtacgc tctaagcggc gacgcggaaa aagccaggga cgcgggggcg
     8041 acggcgtacc tcgccaagcc ctacagcccg cgcgacctgc tggcgaaaat ccgggagctg
     8101 gttcccgaag cttgaacaag gagaggcaat ggcaaagctg acgcgcagga tcgcggtgat
     8161 cgccgccgcg ggggcgttct cgctaccggc cgcggccggc caggaagagc agttcacgcc
     8221 cgagttcgcc gccgtgcgct cgtcgttcga cgagaagcac accccgaagc tcaccgcgcc
     8281 cgactcggtg aagcgcgggc agtggttcga cgtgagtgtg aacgtcggcg ccggcggggt
     8341 gcatccgtcg ctctccgagc accacgtgcg ctacatcgcg ctctacaagg acactgccga
     8401 gatcgcccgc gtctacctgc acccggtgtt ctcggcgccg cgggtcacct tcaccatcgc
     8461 gctcgactcg agcggcgtgc tgcgcgcgct cgccgagccg actcactcgg cggcctggga
     8521 ggcctccaag aagatcaagg tcgcgcctta ggcgcgagcg tcttgatgtc gaagtcagta
     8581 gcaccgccct gcgccccgac cgtgacctcg cgggtcgtct ggccgagggc ctcctgccac
     8641 atgcgcaccc ggtactttcc aggcggcacg ttggcgatgc ggtaggcgcc ggccgcgtcg
     8701 gtcaccgcat agaaggggtg ctcggcgacc acgacccagc tcttcatcca ggagtgcagg
     8761 tcgcaggcga tggtcacgag ctccggcttg gtgaaggtga tcgggatcgt gcggttgtgc
     8821 ggctgcgtgc ggttgaactc gggattctgc ttgctcacgc tgcgcaggtt gtgcagcagc
     8881 cggtcgctgt tcaggaagtc gaccgtgccg ccgaccggga cgacgagcac ccgcggcacg
     8941 aacacgcacc ccttctggtc catctcgacc ttgtccggct gcgccggcca tttcgcgccc
     9001 gcgccagggt tctctatcca gaccaccgcg ttctgcaccc cgcgcttcgg cccgagcacg
     9061 aggtcgtccg cgtccttctc cttgccgcag atgtactggt cgatggtgac cgggaccttc
     9121 ggccgctcgg gaagagcgcc gctgaagagc acggtgccgg tgagatcgcc gcccagcgcc
     9181 cacccgctcg aggccaggca gccggcgaaa aggagaacct tcgccctcat gcagcggcga
     9241 atataccgca acggcccgcg actaggccgc tttccgctcc ttgggtagga tgtcgcacgc
     9301 caccgcgcaa acctgaggag gaaagccatg ggcaagggct acatcgtcgt cacctatagg
     9361 aagatcaagg accaggcgaa gtgggaggcg tacgccaagc tcgcggtcct cgcggtacag
     9421 aagtcgggcg gcagattcat cgtgcgtgcg acgcctacgc gcgtgcacga gctcggcatg
     9481 aaggaacgcg tggtgatcgt cgagttcgac aacgtccaca aggcgatgtc cgcgcacgat
     9541 acgcccggct accaggaggc gctcaaggcg ctgggcgacg gcgccgagcg cgacttccgc
     9601 atcgtcgagg gcgtctgaag acctggtccc gctaagcgct ccagcgcacg gtgccgggcg
     9661 ccgtggcatg agatggtgtc tgccatgatt gcccgagcta tccccaagac cggcccgagg
     9721 cgctgcaggc tgctgtacgt cgaagaccac cccgacagcg tcgcgctggt ggagaccctg
     9781 ctggcaggcc gcaaggacct gctgctgctg cgcgcggcgg atgcgaatct cggaatcaag
     9841 ctggcgcgcg cccagcggcc ggaggcgatc ctgatcgacg tcgacctcgc gggcatgggc
     9901 gccctcgagt ttatgaaggt cctgcgcgcc gacccggcga cggaagcgac gcccatcctg
     9961 gcgctcggcg ccaacgccgc gcccgaggcc atcgtcaagg cgctggaggc cggcttcttc
    10021 cagtacctcg ccaagcccct gcgtgccgag ccgttcatgg aagccctcgt gttcgcgctg
    10081 gaattcgcgg ccgtggagcg ctccgaacac aacgcgttga tgttgcgctt cggccatccc
    10141 cgcacaccct tcaaggagtc ccgatgaaaa tccgtccatt gcacgaccgc gtaatcgtca
    10201 agcgtatcga cgaggaaaag aagaccgctt ccggcatcgt catccccgac accgcggcgg
    10261 agaagcccga ccagggcgag gtcaccgcgg tcggcaaggg aaagaggacc gacgaggggg
    10321 agctcgtgcc cctcgacgtc aaggtcggcg accgggtgct gttcggcaag tactccgggc
    10381 agaccgtccg ggtgaagggc gatgagctcc tcgtaatgcg cgaggaagac atcatgggaa
    10441 tagtcgaagg acaacaatga ccatcaaaga cgtacggttc cacgaagacg caagaacgag
    10501 gatggtccgc gggctgaaca tcctggccga cgcggtgaag atcacgctcg gcccgagagg
    10561 ccgcaacgtg gtgctcgatc gctcctgggg atcgccgacc gtcaccaagg acggcgtgtc
    10621 ggtcgcgaag gagatcgagc tcaaggacaa gttcgagaac atgggcgcgc agatggtgaa
    10681 ggaagtcgcc tccaagacct ccgacgtggc cggcgacggc accaccaccg cgacggtgct
    10741 cgcccaggcg atcgtgcgcg agggcatgaa atacgtcgcc tccggcatga acccgatgga
    10801 cctcaagcgc ggcatcgacc tggcggtcgt cacggtggtc gaggagctga agaagatctc
    10861 caagccctgc gcctcctcca aggaagtcgc gcagatcggg tcgatctccg ccaacctcga
    10921 caccgaggtg ggcaagatca tcgccgacgc gatggagaag gtcggcaagg aaggcgtgat
    10981 caccgtcgag gacggcaagt ctctccacaa cgagctcgag ctggtcgagg gcatgcagtt
    11041 cgaccgcggc tacctctcgc cgtacttcat caacaacccc gagaagcaga tcgccgtgct
    11101 cgaggacgcc tacgtcctgc tgtgcgacaa gaaaatcagc tcgatccgcg acatgctgcc
    11161 gctgctggag caggtcgcca aggcgggcaa gccgctcctg gtcctcgccg aggaagtcga
    11221 gggcgaggcg ctcgccaccc tggtggtgaa caacctgcgc ggcatcctca agacccttgc
    11281 ggtcaaggcg ccgggattcg gcgatcgcag aaaggcgatg ctcgacgaca tcgcggtgct
    11341 caccggcgcg acggtgatct cggaagagct cggcctgcag ctcgagaaga ccacgctcaa
    11401 ggacctcggc cgcgtcaaga aagtcgagag caccaaggag aacaccaccc tcatcgacgg
    11461 cgccggcgac aagaagctga tcgagggccg catcaagcag atccgcctgc agatcgaaga
    11521 gcagaccagc gactacgaca aggaaaagct gcaggagcgc ctggcgaagc tcgccggcgg
    11581 cgtggccctg atcaaggtcg gcgccgcgac cgagatcgag atgaaggaga agaaggcccg
    11641 ggtcgaggac gcgttgcacg cgacccgtgc ggccgtcgag gaaggcgtgg tgccgggcgg
    11701 cggcgtcgcc tacctgcgcg cgcgcgccag gctgaaggat ctcaagggcg ccaaccgcga
    11761 ccaggacggc ggcatccaga tcgtgctgcg cgcgctggaa gagccgctga ggcagatcgt
    11821 cgccaacagc ggcgccgagc cttccgtggt cctcaacaag gtgctcgagg gtaaggacag
    11881 cttcggctac aacgcgcaga ccgccgagtt cggcgatctc gtgcagcaag gcgtcatcga
    11941 tccgaccaag gtcgtgcgct tcgcgctgca gaacgcggct tcggtcgcgg gcctggtgct
    12001 caccaccgcg gcgatggtcg cggagcgcgt cgaggaagac aagggcggcg gccaccaagg
    12061 cggcatgggt gggatgggcg gcatgggcgg gatgggaatg taagggcccg tcccggtcgc
    12121 cccgcgcgat caggcggccg cgttgcgcca gaggctcgcc agccagggct gctgctcgcg
    12181 cggcaggccg ggcgggcggt agtagtggcc gagctcggca aacccggctt gcgtcaggta
    12241 gcggcgccac gatccgaggt cgtggtacac gccgtagcgg ccgcggttcc agccctcctc
    12301 gttacggcca tgcggatttg agctgaacag caccccgcgc ggcttgagcg ccgcgcgcag
    12361 ctctcgcagc actcgcggca gcgcctcacc gggaacatgg aacagcacgg cgttggcgaa
    12421 cacgccgtcg aagcgcccgc cgggcagatc gagcccgagg aaatcctgct ccagcacctc
    12481 gcagccgcta tgcgcgcgcg ccatcgccgc gaaggtgggg gcgccttcca gccccaccgc
    12541 caggtggccg agcgcgctca acgccttcag gtcgcgtccc ggcccgcagc ccagatccag
    12601 gatcgtgaac ggcggcgcgc cttcgatgtg ccgcagcagc tcgccgatgt tctggctgac
    12661 gtcgtgatcg cgcgtgccgc gccagaactc ctccgccccc tgctcgtagt gctccagcgt
    12721 gaccttggcg atgcgcgcga gctcttcggc tttcagcttc ataggccgcg gattatggcg
    12781 cgggatacga tgtagccatg atcgcctggt ttcccggcca catggcctcc gcgcgcaaga
    12841 aggccgcgca gaccatgggc agaatcgacg tggtcgtgga gctgctggac gcgcgccttc
    12901 ccggtgcgag cgcaaatccg ctgatcgagc ggctgcgccg cgacaaacag cgcccctgcc
    12961 tgaagatcct gaacaaggcc gacctggccg acccggacgc gaccgccgcc tggctgcgcc
    13021 actacggcaa cgagaagctg gtgcgggcgg tggcgctctc ctgcaagcgt cccggcgacg
    13081 tggcgaaggt gccgcgcctc gccgcggccc tcgtgccgca ccgcgacagc gcgctcaaac
    13141 ccgtgcgcat gatggtgatg ggcgtgccga acgtcggcaa gtccacgctc atcaacgccc
    13201 tcctcaagcg caaggtggcg aagaccggcg acgagcccgc ggtcacccgc gagcagagcc
    13261 gcttcgagct cgacgagcgc aacgagctca tcgacacgcc gggcctgatg gccccggtga
    13321 tcaagcacgc cgccgacggc ttgatgctcg ccgcgagcca cctcatcggt ccggagtcat
    13381 accaggcaga ggaggtggcg gccttcctcg ccgggatcct gctcgagcgc tatcccgcgc
    13441 agcttaggac gcgctacggc ttcgacactc aaggaatgga cggcgatggc ctgatcgcgg
    13501 cgatcgccgc caagcgcggc tacctcggca aaggcggcac gccgcacctg gagaaagccg
    13561 cgcgcgcgct cctgcaggac taccgcagcg gactgctcgg ccggatgacc ctcgagacgc
    13621 cttcgagtag gcttatcgca taggaggctc acatggaacg tcgtgacttc gtgaagcaaa
    13681 tcggcgcgct cggcg
//
LOCUS       DATBSZ010000242        15264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_659240, whole genome shotgun sequence.
ACCESSION   DATBSZ010000242 DATBSZ010000000
VERSION     DATBSZ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15264
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659240"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..716
                     /locus_tag="VIG70_15330"
     CDS             372..716
                     /locus_tag="VIG70_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY8251972.1"
                     /translation="MELQFYQTAERRRPFQEWLRGVSDMQAVIRIEARLARLRAGNFG
                     DSETVGEGVMELRIDWGPGYRVYFARLGQVVILLLCGGDKRTQHKDIKRAKEYFDDYK
                     ARTAQEKPRGRR"
     gene            787..1011
                     /locus_tag="VIG70_15335"
     CDS             787..1011
                     /locus_tag="VIG70_15335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HEY8251973.1"
                     /translation="MIEDGDQGAIMVALRQVAQAQGGIARVARKARLTREATYKMLSR
                     SGNPELRSLKALLAATGLRLAVRPIEKRSA"
     gene            complement(944..2755)
                     /locus_tag="VIG70_15340"
     CDS             complement(944..2755)
                     /locus_tag="VIG70_15340"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8251974.1"
                     /translation="MLAVTDALETVANDWLARFEGALADCDDALLKTLFRADSHWRDV
                     LALTWDIGTVSGGDAVAAQLKTHAAQVTPTGFRTDPDRTPPRAVTRAGTRCIEAIFRF
                     ETPAGRGSGVLRLLPGESRAWTLLTALDELNGLEEQVGLLRPRGEAYSREFAGPNWLD
                     QRKSAAQYADRDPVVLVVGGGHAGLSIAARLAHRQVDTLIVDRWPRVGDNWRRRYHAL
                     TLHNQVHVNHLPYMPFPPTFPVYIPKDQLANWFEAYAEAMELNYWAGTDFEGGTYDPR
                     EERWSVVLRRPDGAKRELHPRHVVMATGVSGIANLPDIPALRGFRGTVLHSSRYEDGE
                     AWRGKSALVVGTGNSGHDIAQDLHSSGAKVTLVQRSPTLIVNCEPSAQLVYGLYSEGP
                     PLEDCDLITASIPLKVSREGHKLATEHARSLDKPLLDNLERKGFRLCFGEDGTGWQFM
                     YLTRGGGYYFNVGCSDLVAEGKIGLAQFADLERLLEDADLVVLATGWKGQEHLVRKLF
                     GEEVAGRVGPIWGFGDGQELRNMFTRTAQPGLWFIAGSFAQCRIYSKYLALQIKACEA
                     GLLPKNLAGRGFGRDPTPTASQLALRRAEGPLPPGAP"
     gene            complement(2779..3429)
                     /locus_tag="VIG70_15345"
     CDS             complement(2779..3429)
                     /locus_tag="VIG70_15345"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8251975.1"
                     /translation="MKRFAVFTLTLAVAGCSADNPMGHREMGTLIGAGAGAVVGAVAY
                     DKNRTKGAVVGAVGGALAGGAVGRYMDDQKRDLEKNLGAEIKSGQARVEKLPNDVVRI
                     TMTNQTAFETDSSEIKPGFHSTMDKLADVVVRYGKTTLTVVGHTDNVGSDSYNQRLSE
                     RRALSVAQYLESKRVNSMRLATSGKGETVPLANNASESGRQANRRVEIYVEPVVQS"
     gene            complement(3544..3744)
                     /locus_tag="VIG70_15350"
     CDS             complement(3544..3744)
                     /locus_tag="VIG70_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251976.1"
                     /translation="MKTEELLSKLELIEEQARLSLDELPQHLGKERQRMIIALVKHIR
                     WALTDPNSPALVDPEATVRLPS"
     gene            3840..4517
                     /locus_tag="VIG70_15355"
     CDS             3840..4517
                     /locus_tag="VIG70_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEY8251977.1"
                     /translation="MPAGFRVLKRKRRVEAVWVEKFRRIPVANVSDSMFRTSAGGARL
                     RPYHAGGAMAGPALTVRTAPGDNLMVHMALNLAEKGDVLVVDAGGELTNSIIGERMLA
                     YCQAKGFAGIVINGAVRDVGYIRKHDFPVYAAGVTHRGPYKNGPGEINVTVALDGMAI
                     APGDLVLGDDDGLLCVPYDDVEAVYKLAHDKHEMETRTFKDIGKDDNNRAGYEKTLRS
                     LGCEFEP"
     gene            4562..5416
                     /locus_tag="VIG70_15360"
     CDS             4562..5416
                     /locus_tag="VIG70_15360"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HEY8251978.1"
                     /translation="MPAAQRGELGVRWWVSTGETKLSHNAQGLDPTLGNPTSVLVYEN
                     LDANVLELFGRQNFRNNLFLKGTLGIGEVNRGAFDDEDFNAGQVKFSDTTSSVTQGRI
                     GYGTLDLGYQWVLREGAVTLGVFGGFGQWTEDYDAYGASDHLGFIGGDISRDVNVISN
                     KVRWRALRVGFAGNFVFGRARLSLDLAAVPYAQMRNEDSHHLRTDLGPVPNIIDEGEG
                     WGAQLDAELRYEIMRRTELGVGLRFWYMEVRDGTTDFKNHPGVAEAPLVDFYTWRTGV
                     TISLRRTW"
     gene            complement(5426..7537)
                     /locus_tag="VIG70_15365"
     CDS             complement(5426..7537)
                     /locus_tag="VIG70_15365"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY8251979.1"
                     /translation="MAATAPHLDDTRFGYLKPKDVARLAEAYRFSEAAHAGQTRQSGD
                     PYISHPLAVAEILAGWHLDGQTLMAALLHDVTEDTSVTKDEISDTFGRPVAELVDGVS
                     KLDKIEFQSAEDAQAENFRKMLLAMARDVRVMLIKLADRLHNMRTLGAVPPAKRRRIA
                     RETMEIYAPIANRLGLNTLYHELQELAFSHLYPTRFRVLAKATKAARGNRREMIGKTL
                     DAIKKKLADSGVQATVFGREKHVYSTYRKMIEKHLTFSEVHDIFGCRVIVSDVPVCYL
                     ALGALHALYKPIPGKFKDYIAIPKANGYQSIHTDLIGPYGVPVEVQIRTEQMHRLAES
                     GVASHWMYKDDADKLSELQKQTHRWLQSLLEIQHQSGDPQEFLEHVKVDLFPDEVYVF
                     TPKGRILSLPRGATAVDFAYHVHTDIGNRCVAAKVNGELVPLRTELRNGDRVEIITAS
                     HAKPSAGWLQYVRTGKARSNIRHFLKTMQYEESAGLGERLLDQALRGLKGSLAEVDDT
                     AWERVVRDGGARSREELLADIGLGKRLPAVVARRLLKKSDLEDGKATGSVTIRGTEGV
                     AVQLATCCRPIPGDSIVGSIKKGQGLVVHNSDCPAIVRSRKNEPDQWLDVEWDPRTTR
                     LFQAAINVTVENQRGVLAKVASEIAEAASNIDSITMEEDRMVFTTMHFVLEVANRQHL
                     ARVMRALRRLPDVKKISRTRE"
     gene            complement(7636..7863)
                     /gene="rpoZ"
                     /locus_tag="VIG70_15370"
     CDS             complement(7636..7863)
                     /gene="rpoZ"
                     /locus_tag="VIG70_15370"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY8251980.1"
                     /translation="MARITVDDCLKRIPNRFQLTLAATYRARQLGAGASPMVEPNRDK
                     STVIALREIASGKIGTEVLKRNAMGPSTPSL"
     gene            complement(7844..8434)
                     /gene="gmk"
                     /locus_tag="VIG70_15375"
     CDS             complement(7844..8434)
                     /gene="gmk"
                     /locus_tag="VIG70_15375"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY8251981.1"
                     /translation="MNKRKGRLFVITAPSGAGKTSLIEALMRGDPTLKLSISYTTRAP
                     RPGEKDGVDYRFVDDATFLAMRSRGEFLESAEVHGYRYGTSKGFITSALAKGEDLILE
                     IDWQGARQVRAIYPDCVGIFILPPSIEELERRMRSRGQDSDAVIRRRLDNAREELEHT
                     GEFEYRIINKDFDTARQELAEIIRKERAKPHGAHHR"
     gene            complement(8427..9296)
                     /locus_tag="VIG70_15380"
     CDS             complement(8427..9296)
                     /locus_tag="VIG70_15380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY8251982.1"
                     /translation="MSLPVQSMTGYAAASSGSPRGTLSLELRSVNSRFLDLQFRIADE
                     LRAHEPMLRELISAGVARGKVDCRLSLAEGAAGANPALNAEALARLKALAEAAKKAVP
                     DAAPLRIADLLRWPGVISEPADEESLRASVAGLCRRALADLAAARSREGAKLASGIVE
                     RVRVMRIRVDDVAPLMPQAIAAYQERIAERLREAIGSADDERIRTEVALFAARGDVAE
                     ELERLRVHLGEVERVLGAGGLVGKRLDFLAQELNREANTLASKALSQAISDCALELKL
                     LVEQVREQVQNLE"
     gene            9318..10208
                     /locus_tag="VIG70_15385"
     CDS             9318..10208
                     /locus_tag="VIG70_15385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8251983.1"
                     /translation="MPSQANTPLPDDSILQNYRIVRLLAVGGFSFVYLAHDLNDAPVA
                     IKEYLPSTLALRVNGERHPQIPEDDLAAFRAGMQCFFEEAGALAHLEHPNVVRVLDFF
                     RANDTVYLVMRYESGLSLDEHIKDRRGTPDELWVRSTFAQLLDGLREVHAHKLLHLDI
                     KPANVYVRDDGTPVLIDFGAARQVLSAGDMKLPLYTPGFAAPEMHLAREMLGPWSDIY
                     SVGATMYACLGAAAPQAADARVENDLLVPASRAFAGRYEPELLEIVDWCLNLIHVQRP
                     QSVLALQKALLGKKSDYRPG"
     gene            10177..10917
                     /locus_tag="VIG70_15390"
     CDS             10177..10917
                     /locus_tag="VIG70_15390"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEY8251984.1"
                     /translation="MEKSPTIVQVSVVGARDINEDRVAHWSAADTLLLAVADGLGGHM
                     HGEVAAQVAVDVLGAAFLGEARQRLEDPTGFLPRSLAAAHAAIVREGEKRRLPDNPRT
                     VIAACVVQEDRVYWTHVGDCRFYLLRAGRVLARSRDHTIVQRLVDEGRIREEAMATHP
                     HRNRLLACLGGFHMPRIEPVTSVVLQRDDIVLLCSDGFWGPLTERQLVEAIASRPLAE
                     AIQELVAVAEVSAGPDCDNVSVVAMTWN"
     gene            10908..11633
                     /gene="rph"
                     /locus_tag="VIG70_15395"
     CDS             10908..11633
                     /gene="rph"
                     /locus_tag="VIG70_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY8251985.1"
                     /translation="MELTRPNGRRAAELRPVRIQRRYTKHAEGAVLVEFGDTRVLCTA
                     SVEERVPAFLKESGRGWVTAEYGMLPRSTNTRTEREAARGKQSGRTQEIQRLIGRSLR
                     AVVNLASLGQRTVHIDCDVLQADGGTRTAAITGAFVALHDALSFLKGKNLLQAWPIRD
                     HVAAVSVGVAKGTPMLDLDYSEDSACGCDMNVVMTGAGRFVEVQGTAEGEAFSRAELD
                     RLLELAAGGIAELIQHQKKSLGL"
     gene            11630..12223
                     /gene="rdgB"
                     /locus_tag="VIG70_15400"
     CDS             11630..12223
                     /gene="rdgB"
                     /locus_tag="VIG70_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY8251986.1"
                     /translation="MKLVLASSNPGKLREIGALLAQFSMELVPQSSLGIAETGEPHAT
                     FLENALAKARHASRAAGLPALADDSGLCVDALGGEPGVHSAYYAGADASREERDARNN
                     QKILLNLSDNRKAYYACIMVFLRHPEDPRPLVAEGVWQGEIARAPRGANGFGYDPLFF
                     VPSLQKTAAELAPEHKNRISHRGIALRKLIGLLNDEA"
     gene            12210..13421
                     /gene="hemW"
                     /locus_tag="VIG70_15405"
     CDS             12210..13421
                     /gene="hemW"
                     /locus_tag="VIG70_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY8251987.1"
                     /translation="MTKLDALPPLALYVHIPWCVRKCPYCDFNSHERAGALPENEYVA
                     RLMLDLEGLLPLVWGRRLVSVFIGGGTPSLFSPGAIDTLLSGVRARMTLEPGAEITLE
                     ANPGTVEAARFRGFREAGVNRISIGVQSFEERMLAALGRIHGPGEARRAIEAALASFD
                     NVNIDLMYGLPGQSVAMARADIEEAARAGAPHISAYQLTIEPNTAFWSRPPSLPEHDL
                     CADMQVMIEETLGRAGYEHYETSAFARPGRRCQHNLNYWEFGDYLGIGAGAHGKISFP
                     DRITRHERAKQPATYLQGSSTVENRVVPPHELPFEFMLNALRLVEGFPPVLFEERTGL
                     PFTILEKQLQKSEQQSLLERDWNRVRPTARGQRFLNELLALFLADETEDPSPGGRGAG
                     RVIRISSPASR"
     gene            complement(13315..13971)
                     /gene="trmB"
                     /locus_tag="VIG70_15410"
     CDS             complement(13315..13971)
                     /gene="trmB"
                     /locus_tag="VIG70_15410"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEY8251988.1"
                     /translation="MNRPIRSYVLRQGRTTPAQKRALEELLPKYGIPFSDQKIVSSRS
                     LVLEIGSGMGETTVEIAKAHPDTDFIAVEVHGPGVGSLLNRIHSEQLKNLRVIRHDAL
                     DVLEHMIADGSLAAIHLFFPDPWPKKRHHKRRMVQPEFAALAAAKLGEGGILHAATDW
                     PAYAEHMSEVFSREPLLEPAAAGFTARPVTKFESRGRRLGHPVRDLLFRRRGKAPAAR
                     "
     gene            complement(13968..14768)
                     /locus_tag="VIG70_15415"
     CDS             complement(13968..14768)
                     /locus_tag="VIG70_15415"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY8251989.1"
                     /translation="MILKDSLNIAGKSYGSRLLVGTGKYRDFAQTRAAVEASGAAIVT
                     VAIRRTNIGQNANEPSLLDALPPSKFTYLPNTAGCYNAADAVRTLRLARELLDGHDLV
                     KLEVLGDPQTLYPNMPETLKAAGELVKDGFKVMVYCSDDPIQAQMLEQTGCVAVMPLA
                     SLIGSGMGILNPWNLQLVMEKVKMPVIVDAGVGTASDAAIAMELGCDGVLMNTAIAAA
                     RDPIRMAHAMKLAVEAGREAYLAGRMPKKLYSATPSSPTEGKIAPKAA"
     gene            complement(14765..14965)
                     /gene="thiS"
                     /locus_tag="VIG70_15420"
     CDS             complement(14765..14965)
                     /gene="thiS"
                     /locus_tag="VIG70_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY8251990.1"
                     /translation="MEVTVNGTAHRLEKPLQVLGLLEKLELVGKKVAVERNGEIVPKS
                     AHASTVLADGDRLEIVVAVGGG"
     gene            complement(14965..15183)
                     /locus_tag="VIG70_15425"
     CDS             complement(14965..15183)
                     /locus_tag="VIG70_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8251991.1"
                     /translation="MIQRVLSALLGVLFLVAVFVFTSILVAIALTAGLLLAGWVWWRS
                     RGRVPERRGQVIEGEYREVRDRNYHLRP"
BASE COUNT         2467 a         5040 c         5161 g         2596 t
ORIGIN      
        1 cggatgcggc gccggcggcg agcatgacca cgacggcggt tgcggcgaat cggcggatca
       61 tttcgggtct cctcccgttg agacgcgata cacgcgccga ttctgccaca cgacgcccgc
      121 gcgaaggcgc gccaggttcc ccaggcgtcg cgccgttgat tcgaaacgaa tccctcggtc
      181 gagcggcttc gccgaaccgg tgaccgtctt ttcgatagcc gccgcaaacc cttagtcgcg
      241 cgccacggcc tttttccctc ggccccgtca tcgaacgcac gcctcgtgcg ttcaatatgt
      301 catgcgcgtc aagggctggg ggaatggtac ggaagctctg gcgcatctgt gaactataag
      361 ttacaattac cgtggagctt cagttctatc agaccgccga gcgcagaagg ccattccagg
      421 agtggctgcg aggcgtaagc gacatgcaag ccgtcatacg catcgaagcc cggcttgcgc
      481 gtctacgcgc agggaacttc ggtgactccg agaccgtcgg cgagggtgtg atggagctca
      541 ggatcgattg ggggccggga tatcgtgtgt acttcgcccg gcttgggcag gtggtgatcc
      601 tcctcctttg cggaggagac aagcggacgc agcacaagga tatcaagcgt gccaaagagt
      661 acttcgacga ctacaaagcg cgtacagcgc aggagaaacc gcgcggtcgc cgctagcgtt
      721 ccctacgagc cttggcttat cgaacagcta aaggacccag ggcacgcggc cgcatatctt
      781 gaagcggtga tcgaggacgg cgaccaaggc gccatcatgg ttgcgcttcg tcaagtcgcc
      841 caagcgcaag gaggtatcgc tcgggttgcg cgaaaagcga gactgacgcg agaggcgacc
      901 tacaaaatgc tgtccaggtc gggaaacccg gagctgcgca gtctcaaggc gctcctggcg
      961 gcaacgggcc ttcggctcgc cgtaaggcca attgagaagc ggtcggcgta gggtctctac
     1021 cgaatccacg gcctgccaaa tttttaggca gcaggcccgc ctcgcaggcc ttgatctgca
     1081 gcgcgaggta tttcgagtag atgcggcatt gcgcgaagct tccggcgatg aaccacaacc
     1141 ccggctgcgc ggtgcgcgtg aacatgttgc gcagctcctg cccgtcgccg aatccccaga
     1201 tcgggccgac gcggccggcg acctcctcgc cgaacagctt gcggaccaga tgctcctggc
     1261 ccttccagcc ggtggcgagc acgacgagat ccgcatcttc gagcaggcgc tcgaggtcgg
     1321 cgaactgcgc gagcccgatc ttgccctcgg cgacgagatc ggagcagccg acgttgaagt
     1381 agtagccgcc gccgcgggtg aggtacatga actgccatcc ggtgccgtcc tcgccgaaac
     1441 atagcctgaa gcctttgcgc tcgaggttgt ccagcagagg cttgtccagg ctccgggcgt
     1501 gctccgtcgc gagcttgtga ccttccctgg agactttcaa gggaatcgat gcggtgatca
     1561 ggtcgcagtc ctcgagcggc gggccctcgc tgtacagccc gtagacgagc tgagcgctcg
     1621 gctcgcaatt gacgatcagc gtcgggctgc gctggacgag ggtgaccttc gccccgctcg
     1681 agtgcaggtc ctgtgcgatg tcgtggccgc tattgccggt gccgacgacg agcgcgctct
     1741 tgccccgcca cgcctcgccg tcctcgtacc ggctggaatg caggacggtg ccgcggaagc
     1801 cgcgcagggc gggaatatcg gggaggttcg cgatgccgct gacgccggtc gccatcacca
     1861 cgtggcgcgg gtgcagctcg cgcttggcgc catctgggcg gcgcagcacg accgaccagc
     1921 gctcttcgcg cggatcgtac gtcccgccct cgaagtcggt ccctgcccaa tagttgagct
     1981 ccatcgcctc cgcgtaagcc tcgaaccagt tggcgagctg gtctttcggg atgtacaccg
     2041 ggaaggtcgg ggggaagggc atgtacggca ggtggttgac gtgcacctgg ttgtgcagcg
     2101 tcagcgcgtg gtagcgcctg cgccagttgt cgcccacgcg cggccagcga tcgacgatca
     2161 gggtgtcgac ctgacggtgc gccagccgcg cggcgatcga aagcccggca tgcccgccgc
     2221 ccaccaccag gaccacgggg tcgcggtccg cgtattgggc ggcggacttc cgttggtcga
     2281 gccagttggg gccggcgaac tcgcgcgagt acgcttcgcc ccgggggcgc agcaggccga
     2341 cctgctcctc gagcccgttc agctcgtcga gcgcggtgag cagcgtccat gctctggatt
     2401 cgccgggcag caaacgaagc actccgcttc cccggcccgc gggggtttcg aagcggaaga
     2461 tcgcctcgat acaccgggtg cccgcgcgcg tcaccgcgcg gggcggcgtg cgatcgggat
     2521 cggtccggaa accggtcggc gtgacctggg cggcgtgtgt tttcagctgc gcagcgaccg
     2581 cgtcgccgcc gctcaccgtg ccgatgtccc aggtcagagc gagaacgtcg cgccagtggc
     2641 tgtcggcacg aaacagggtt ttcagcagcg cgtcgtcgca atccgcgaga gcgccctcga
     2701 acctcgcgag ccaatcgttc gctaccgtct ccagggcgtc tgtgacagcc agcacgaccc
     2761 ggcaacgata cgcgaaatct agctctggac tacgggctcc acgtaaatct cgacccggcg
     2821 attcgcctgc cggccgctct cggaagcgtt gttcgccaac ggcacggtct cgcctttgcc
     2881 ggaggtggcg aggcgcatgg agttcacgcg cttcgattcg aggtactgcg cgaccgacag
     2941 cgcgcggcgc tcggagagcc tctggttgta ggagtcggag ccgacgttgt cggtgtggcc
     3001 cacgacggtg agggtggtct tgccgtagcg caccacgacg tcggcgagct tgtccatggt
     3061 gctgtgaaag ccgggcttga tctcggacga atcggtctcg aacgcggtct ggttggtcat
     3121 ggtgatgcgc accacgtcgt tcgggagctt ctcgacgcgc gcctgcccgg acttgatctc
     3181 ggcgccgagg ttcttctcga ggtcgcgctt ctggtcgtcc atgtagcgcc cgacggcgcc
     3241 gccggcgagc gcgccgccca cggcgccgac gaccgcgccc ttggtgcggt tcttgtcgta
     3301 ggccaccgcg ccgaccacgg ctcccgcgcc tgcgccgatc agcgtgccca tctcgcgatg
     3361 ccccatcggg ttgtcggcgg agcagccggc gacagcaagg gtgagggtga agacagcaaa
     3421 gcgtttcatg tgagctcctt ctttcgaaaa gcaggaattc cggaaattct agtcaggcga
     3481 taccaacgcc gctcgggcac atctcacgcc cgcgatcaaa aagaagcggt ggtgcggctg
     3541 ctgctaggaa ggaagacgga ccgtggcctc gggatcgacg agcgctggcg agttcggatc
     3601 ggtgagcgcc cagcggatgt gcttgaccag cgcgatgatc atgcgctggc gctccttccc
     3661 gagatgctgc gggagctcat ccagcgagag gcgcgcctgc tcttcgatga gctccagctt
     3721 cgacagcaat tcttcggttt tcatgccggc gagcctagca aaggcgatgt gaagttgcac
     3781 tctgtcggga tttggcgaca gctatactgc gacgtccacg tcgagggagg aacaaggaaa
     3841 tgccagcggg gttcagggtt ctcaagcgca agcgccgggt tgaagctgtg tgggtggaga
     3901 agtttcgccg cattccggtg gccaacgtca gcgattccat gttccgcacg agcgcgggcg
     3961 gcgcgcgcct gcgcccgtac cacgccggcg gcgcgatggc cgggccggcg ctcaccgtgc
     4021 gcaccgcccc gggcgacaac ctgatggtgc acatggcgct caacctcgcc gagaaaggcg
     4081 acgtgctcgt ggtcgacgcc ggcggcgagc tcacgaactc gatcatcggc gagcgcatgc
     4141 tcgcgtactg ccaggcgaag ggcttcgccg gcatcgtcat caacggcgcg gtgcgcgacg
     4201 tgggctacat ccgcaagcac gactttccgg tgtacgccgc gggggttacg caccgcgggc
     4261 cctacaagaa cggaccgggc gagatcaacg tcaccgtggc gctcgacggc atggcgatcg
     4321 ctccaggaga cctggtgctc ggcgacgacg acggcctgct gtgcgtgccc tacgacgacg
     4381 tcgaggcggt gtacaagctc gcgcacgaca agcacgagat ggagacccgg accttcaagg
     4441 acatcggcaa ggacgacaac aaccgcgcgg ggtacgagaa gacgctgcgg agcctgggtt
     4501 gcgagttcga gccctagtct tcgcgctcgt tttttcggcg aatgccttcg gcgaggaggc
     4561 gttgccggcc gcgcaacgcg gcgagctcgg cgtgcgctgg tgggtgagca ccggcgagac
     4621 caagctcagc cacaatgcgc agggcctcga tccgacgctc ggcaatccga cttcggtgct
     4681 ggtctacgag aacctggatg cgaacgtgct ggagctcttc gggcgccaga acttccgcaa
     4741 caacctgttt ctcaagggca cgctcggcat cggcgaggtc aaccggggcg cgttcgacga
     4801 cgaggacttc aacgccgggc aggtcaagtt ctcggacacc acctcgtcgg tgacccaggg
     4861 gcgcatcggc tacggcacgc tcgatctggg ctaccagtgg gtgctgcgcg aaggcgcagt
     4921 cacgctcgga gtattcggcg gcttcggcca atggaccgag gactacgatg cctacggcgc
     4981 gagcgaccac ctcggcttca tcggcggcga catcagccgc gacgtcaacg tgatcagcaa
     5041 caaggttcgc tggcgggcgt tgcgcgtcgg attcgccggc aacttcgttt tcgggcgcgc
     5101 ccggctctct ctcgacctgg cggcggtgcc gtacgcccag atgcgcaacg aggacagcca
     5161 ccacctgcgc accgacctcg ggccggtgcc gaacatcatc gatgaaggcg agggctgggg
     5221 cgcgcagctg gacgccgagc tgcgctacga gatcatgcgg cgcaccgagc tcggcgtcgg
     5281 cttgcgcttc tggtacatgg aggtgcgcga cggcacgacc gacttcaaga accatccggg
     5341 cgtggccgag gctccgctgg tggacttcta cacctggcgt accggggtca cgatcagcct
     5401 gcggcgtacc tggtaacggc ggaacctact cccgcgtccg ggagatcttc ttcacatcgg
     5461 gaaggcggcg cagcgcgcgc atgacgcgcg cgaggtgctg gcggtttgcc acctcgagca
     5521 cgaaatgcat ggtggtgaag accatccggt cttcctccat ggtgatcgag tcgatgttgg
     5581 aagccgcttc ggcgatctcg gaggcgacct tggcgagcac gccgcgctgg ttctcgaccg
     5641 tgacgttgat cgccgcctgg aacaggcgcg tggtgcgcgg gtcccattcg acgtcgagcc
     5701 actggtcggg ctcgttcttg cgcgagcgca cgatcgcggg gcagtccgag ttgtgcacca
     5761 ccagcccttg ccccttcttg atcgagccga cgatcgagtc gcccgggatc ggccggcagc
     5821 aggtggcgag ctgcaccgcg acgccctcgg tgccgcggat cgtgacgctg ccggtggcct
     5881 tgccgtcctc aaggtcggac tttttcagaa gccggcgggc gaccaccgcc ggcaggcgct
     5941 tgcccaggcc gatgtcggcg agcagctctt cgcgcgagcg cgcgccgccg tcgcgcacca
     6001 cgcgctccca ggcggtgtcg tcgacctcgg cgagcgaacc cttgaggccg cgcagcgcct
     6061 gatcgagcag gcgctcgccc agcccggccg actcttcgta ctgcatggtc ttcaggaagt
     6121 ggcggatgtt ggagcgcgcc ttgccggtgc gcacgtactg cagccagccg gcgctcggct
     6181 tggcgtggct cgcggtgatg atctcgacgc ggtcgccgtt gcgcagctcc gtgcgcagcg
     6241 gaaccagctc gccgttcacc ttggccgcca cgcagcggtt cccgatgtcg gtatgcacgt
     6301 ggtaggcgaa gtcgaccgcg gtggcgccgc gcggcagcga caggatcctg cccttgggcg
     6361 tgaagacgta gacctcgtcg gggaacagat ccaccttcac gtgctcgagg aactcctgcg
     6421 ggtcgcccga ctggtgctgg atctcgagca gcgactgcag ccagcggtgg gtctgcttct
     6481 gcagctcgga gagcttgtcg gcgtcgtcct tgtacatcca gtgcgacgcg acgccggatt
     6541 cggcgaggcg gtgcatctgc tcggtgcgga tctgcacctc gaccggcacg ccgtaggggc
     6601 cgatcaggtc ggtgtggatc gactggtagc cgttcgcctt cgggatcgcg atgtagtcct
     6661 tgaacttgcc cgggatcggc ttgtagagcg cgtgcagcgc gccgagcgcg aggtagcaga
     6721 ccggaacgtc gctcacgatc acgcggcagc cgaagatgtc gtgcacttcc gagaacgtga
     6781 ggtgcttctc gatcatcttg cgataggtgg agtacacgtg cttctcgcgg ccgaacacgg
     6841 tcgcctggac gccgctgtcg gcgagcttct tcttgatcgc atccagcgtc ttgccgatca
     6901 tctcgcggcg gttgccgcgc gccgccttag tggccttggc gagcaccctg aagcgcgtcg
     6961 ggtagaggtg cgagaaggcg agctcctgaa gctcgtggta gagggtgttc agccccaggc
     7021 ggttcgcgat cggggcatag atttccatcg tttcccgggc gatcctcctg cgtttcgcgg
     7081 ggggtacggc cccgagcgtg cgcatgttgt gcagccggtc cgcgagcttg atcagcatga
     7141 cgcgcacgtc gcgcgccatg gcgagcagca tcttgcggaa gttctcggcc tgcgcgtcct
     7201 cggccgactg gaactcgatc ttgtcgagct tggagacccc gtcgaccagc tccgccaccg
     7261 gccgcccgaa cgtgtccgag atctcgtcct tggtgaccga agtgtcctcg gtgacgtcgt
     7321 gcagcagcgc ggccatcagg gtctggccgt cgaggtgcca tccggccagg atctcggcga
     7381 ccgccagcgg gtgcgagatg tacgggtcgc ccgactgccg cgtctggccg gcgtgcgcgg
     7441 cttcggaaaa gcggtaggcc tcggcgagcc gcgcgacgtc cttcggcttc aggtagccga
     7501 agcgggtgtc gtcgaggtgg ggcgcagtcg ccgccatggg gtcagattac ccgaccccaa
     7561 caaaatcagg gacagaccac gttttcctga ccggccaggt caggcggaaa aacgtggtct
     7621 gtccctgatt ttgacttaca ggctgggcgt actgggcccc atcgcgttgc gtttgagcac
     7681 ctctgtgccg atcttgccgg aggcgatctc gcgcagcgcg atcacggtgg acttgtcgcg
     7741 gttcggctcg accatcggcg aggcgccggc gccgagctgg cgtgcgcggt aggtcgccgc
     7801 cagcgtgagc tggaagcggt tggggattct cttcaggcag tcgtcaacgg tgatgcgcgc
     7861 catggggttt tgctctttcc ttccggatga tttccgccag ctcttgccgt gcggtgtcga
     7921 agtctttgtt aattatacgg tattcgaact cgccggtgtg ttccagctct tcgcgcgcat
     7981 tgtccaggcg gcggcggatg acggcgtcgg agtcctggcc gcggctgcgc atgcgccgct
     8041 cgagctcctc gatcgacggc ggcaggatga agatgccgac gcaatcgggg tagatcgcgc
     8101 gcacctggcg cgccccctgc cagtcgatct ccagaatgag gtcctcgcct ttggcgagcg
     8161 ccgaggtgat gaaccccttc gacgtcccgt agcggtagcc gtgcacctcg gcgctctcga
     8221 ggaattcgcc gcggctgcgc atggcgagga aggtggcgtc gtccacgaag cgatagtcga
     8281 cgccgtcttt ctcgcctggc cgcggagcgc gggtggtata ggagatcgag agcttcaggg
     8341 tcggatcgcc gcgcatcagc gcctcgatga gcgaggtctt gccggccccg gaaggcgccg
     8401 tgatgacgaa gagacgccct tttcgcttat tcaaggttct gcacttgctc gcggacctgc
     8461 tcgacgagga gcttgagctc cagcgcgcaa tccgagatcg cctggctgag cgccttggag
     8521 gcgagggtgt tggcctcgcg gttcagctcc tgcgcgagga aatcgagccg cttgccgacc
     8581 agcccgccgg cgccgaggac gcgctcgacc tcgcccaggt gcacgcgcag gcgctcgagc
     8641 tcctccgcca cgtcgccgcg ggcggcgaac agggcgactt ccgtgcggat gcgctcgtcg
     8701 tcggccgagc cgatcgcctc gcgcaggcgc tcggcgattc tctcctggta cgcggcgatg
     8761 gcctgcggca tcaggggcgc gacgtcatcg acgcggatgc gcatcacgcg cacccgctcg
     8821 acgatcccgg aagcgagctt ggcgccttcg cgcgagcgcg ccgcggcgag atccgccagg
     8881 gcgcgccggc acagcccggc taccgaggcg cgcaggcttt cctcgtcggc gggctcgctg
     8941 atcacgcccg gccagcgcag caggtccgcg atgcgcagcg gcgcggcgtc gggaacggct
     9001 tttttcgccg cttcggccag cgccttgagg cgcgcgagcg cctcggcgtt caatgcagga
     9061 ttggcgcccg ccgcgccttc ggcgagcgac aggcggcagt cgaccttgcc gcgcgccacc
     9121 ccggcggaga tcagctcgcg cagcatcggc tcgtgcgcgc gcagctcatc cgcgatccga
     9181 aattgcaggt cgaggaagcg cgagttcacc gaacgcagct cgaggctcag cgttccgcgg
     9241 ggagaaccgg acgacgccgc ggcgtagccg gtcatgcttt gaacgggaag ggacaagcgc
     9301 gggctatcat agggtagatg ccatcgcaag ccaacacgcc gcttcccgac gactcgattc
     9361 tgcagaacta ccgcatcgtg cgcctgctgg cggtcggcgg cttttccttc gtctacctgg
     9421 cgcacgacct gaacgacgcg ccggtcgcga tcaaggaata ccttccctcg acgctcgcgc
     9481 tgcgcgtgaa cggtgagcgc catccccaga ttcccgagga tgacctggcg gcgtttcgtg
     9541 ccggcatgca gtgcttcttc gaggaggcgg gcgcgctcgc ccacctcgag caccccaacg
     9601 tggtgcgcgt gctcgatttc ttccgcgcca acgacaccgt ctatctcgtg atgcgctacg
     9661 agagcggcct ctcgctcgac gagcacatca aggaccgccg cggcacgccc gacgagctct
     9721 gggtccgcag caccttcgcg cagctgctcg acggcctgcg cgaagtgcac gcgcacaagc
     9781 tcctgcacct cgacatcaag ccggcgaatg tctacgtgcg cgacgacggc acgccggtgc
     9841 tcatcgactt cggcgctgcg cgccaggtgc tgtcggccgg ggacatgaag ctgccgctct
     9901 atacaccggg cttcgccgcg ccggagatgc acctcgcgcg cgagatgctc ggcccctgga
     9961 gcgacatcta ctcggtcggc gcgacgatgt atgcctgcct cggcgccgcc gcgccgcagg
    10021 ctgcggatgc gcgggtggag aacgacctcc tggtgccggc gagccgcgcc ttcgccggcc
    10081 ggtacgagcc ggagctgctc gagatcgtcg attggtgcct gaatctcatc cacgtgcagc
    10141 gaccgcagag cgtgctggcg ttgcagaaag ccctgcttgg aaaaaagtcc gactatcgtc
    10201 caggttagcg tcgtcggcgc gcgcgatatc aacgaggatc gcgtggcgca ctggagcgcg
    10261 gccgacacgc tgctgctggc ggtggccgac gggctgggcg gccatatgca tggcgaggtg
    10321 gcggcgcagg ttgcggtcga cgtcctcggc gccgcgttcc tgggagaggc gcggcagcgg
    10381 ctcgaggatc cgacggggtt cctgccgcgc tcgctcgcgg cggcgcacgc ggcgatcgtg
    10441 cgcgagggag agaagcgcag gctgccggac aacccgcgca ccgtgatcgc cgcctgcgtg
    10501 gtgcaggagg accgcgtgta ctggacccac gtcggcgatt gccggttcta cctgctgcgc
    10561 gcgggccgcg tcctcgcgcg ctcgcgcgac cacaccatcg tgcagcggct ggtcgacgag
    10621 ggccgcatcc gcgaggaagc gatggccacg cacccgcacc gcaaccggct gctcgcctgc
    10681 ctcggcggct tccacatgcc gcgcatcgag ccggtgacga gcgtcgtgct ccagcgcgac
    10741 gacatcgtgc tgctctgctc cgacggcttc tgggggccgc tcaccgagcg gcagctggtg
    10801 gaggcgatag cgtcgcggcc gctcgccgaa gcgatccagg agctcgtcgc ggttgccgag
    10861 gtaagcgccg gacccgactg cgacaacgtt tccgtggtgg ccatgacatg gaactgaccc
    10921 gcccgaacgg caggcgcgcg gcggagctgc ggcccgtccg gatccagcgc cgctacacga
    10981 agcacgccga gggcgcggtg ctcgtggagt tcggcgatac ccgcgtgctg tgcacggcga
    11041 gcgtcgagga gcgcgtgccg gctttcctga aagagagcgg gcgcggctgg gtgaccgcgg
    11101 agtacggcat gctgccgcgc tcgaccaaca cccgaaccga gcgcgaggcg gcgcgcggca
    11161 agcagtccgg gcgcacccag gagatccagc gcttgatcgg acgctcgctc cgcgcggtcg
    11221 tgaacctcgc gtcgctcggg cagaggaccg tccacatcga ctgcgacgtg ctccaggccg
    11281 acggtggcac gcgtaccgcc gcgatcaccg gggcattcgt ggctctgcat gacgctcttt
    11341 cgttcctgaa gggaaaaaac ctgctccagg cgtggccgat ccgcgaccac gtcgccgccg
    11401 tctccgtcgg cgtggccaag ggcacgccga tgctcgacct cgactacagc gaagattcgg
    11461 cctgcggttg cgacatgaac gtggtcatga ccggcgccgg gcgcttcgtc gaagtccagg
    11521 gcacggcgga gggcgaggcc ttctcgcgcg ccgagctcga ccggctgctc gagctcgccg
    11581 cgggcggcat cgccgagctg atccagcacc agaaaaaatc cctcggactg tgaagctggt
    11641 cctggcttct tccaaccccg gcaagctgcg ggagatcggc gcattgcttg cgcagttttc
    11701 gatggaattg gtacctcagt cgtcgctcgg gatcgccgaa accggagagc cccacgccac
    11761 ttttctcgag aatgcgctcg ccaaggcgcg tcatgccagc cgcgccgccg ggctccccgc
    11821 gctcgccgac gactccggcc tgtgcgtcga cgccctcggc ggcgagcccg gtgtgcattc
    11881 ggcctattac gcgggcgctg atgccagccg cgaagagcgt gacgctcgca acaaccaaaa
    11941 gatcctcttg aatttgagcg acaaccgaaa agcctattac gcctgcatca tggtctttct
    12001 gcgccacccg gaagatccgc ggccgctggt cgccgaaggc gtctggcagg gcgagatcgc
    12061 gcgcgcgccg cgcggcgcca acgggttcgg ctatgacccc ctgttcttcg ttccttctct
    12121 gcaaaaaacc gctgcagagc tcgctcccga gcacaagaac cgcatcagcc accgggggat
    12181 cgcactgcgc aagctgatag ggttgttgaa tgacgaagct tgacgcgctg ccgccgctcg
    12241 cgctctacgt ccatatcccc tggtgcgtgc gcaagtgccc gtactgcgac ttcaactcgc
    12301 acgagcgcgc gggcgcgctg ccggaaaacg aatacgtcgc aagactgatg ctcgacctag
    12361 aggggctgct tcccctggtg tggggacggc gcctcgtcag cgtgttcatc ggcggcggca
    12421 cgccgagcct gttctcgccc ggggcgatcg acacgctgct gtccggcgtg cgcgcccgca
    12481 tgaccctcga gcccggcgcc gagatcacgc tggaagcgaa tcccggcacg gtcgaggccg
    12541 cgcgcttccg cggcttccgc gaagccggcg tcaaccgcat ctcgatcggc gtgcagagct
    12601 tcgaggagcg catgctcgcc gcgctcggcc gcattcacgg ccccggcgag gcgcgccgtg
    12661 cgatcgaggc cgcgcttgcc agcttcgaca acgtcaacat cgacctgatg tacggcctgc
    12721 ccggccagag cgtcgccatg gcacgcgccg atatcgagga agccgcgcgc gccggcgcgc
    12781 cgcacatctc ggcatatcag ctcaccatcg agcccaacac cgcgttctgg agccggccgc
    12841 cgtcgctgcc tgagcacgat ctctgtgccg acatgcaggt catgatcgaa gaaaccctgg
    12901 gacgcgcggg ctacgagcac tacgagactt ccgcattcgc gaggcccggg cgccgctgcc
    12961 agcacaacct caactactgg gagttcggcg actacctcgg catcggcgcc ggcgcgcacg
    13021 gcaagatcag cttcccggac cggatcaccc ggcacgagcg tgcgaagcag cctgcgacgt
    13081 atctacaagg aagttctacg gtggaaaatc gggtcgttcc gccgcacgag ctgccgttcg
    13141 aattcatgct gaacgcgctg cgcctggtcg aaggctttcc gccggtgctg ttcgaagagc
    13201 gcaccggcct gccgttcacg attctcgaga agcagctcca aaaatcagaa caacagagct
    13261 tgctcgagcg cgactggaac cgcgtgcggc cgacggcgcg cggccagcgc ttcctcaacg
    13321 agctgctggc gcttttcctc gccgacgaaa cagaagatcc ctcaccgggt ggccgaggcg
    13381 ccggccgcgt gattcgaatt tcgtcaccgg cctcgcggta aagcccgcgg ccgcgggctc
    13441 gaggagcggc tcgcgcgaga acacctcgct catgtgctcg gcgtacgccg gccagtcggt
    13501 ggcggcgtgc aggatcccgc cttccccgag cttcgccgcc gccagggcgg caaactccgg
    13561 ctgcaccatg cggcgcttgt ggtggcgctt cttcggccag gggtccggaa aaaaaagatg
    13621 gatcgcggcg agcgacccgt cggcgatcat gtgctcgaga acgtcgagcg cgtcgtggcg
    13681 gatgacgcgc agatttttca attgctcgga atgaatcctg ttcaggagac ttccgacccc
    13741 ggggccgtgg acctcgaccg cgatgaagtc cgtgtcggga tgcgccttgg cgatctcgac
    13801 tgtcgtttcc cccatccccg agccgatttc cagcaccagg ctccgggagg aaacaatctt
    13861 ctggtcggaa aacgggatgc cgtacttcgg aagcagctct tcgagcgccc gcttctgcgc
    13921 gggagtcgtc ctgccctggc gcagaacgta gctgcggatc ggccgattca agcggccttc
    13981 ggtgcgatct ttccttcggt gggcgagctt ggcgtcgccg agtagagctt cttcggcatg
    14041 cggccggcga ggtatgcctc gcggccggct tcgaccgcca acttcatggc gtgcgccatc
    14101 cggatcgggt cgcgcgcggc ggctatggcg gtgttcatga ggacgccatc gcagcccagc
    14161 tccatcgcga tcgcggcatc cgaggcggtg cccacgccgg catcgacgat caccggcatc
    14221 ttgaccttct ccatgacgag ctgaaggttc cacgggttga ggatgcccat ccccgagccg
    14281 atgagcgaag cgagcggcat gaccgcgacg cagcccgttt gctcgagcat ttgcgcctgg
    14341 atcgggtcgt cggagcagta caccatcacc ttgaagccgt ccttcaccag ctcgccggcc
    14401 gccttcagcg tctccggcat gttggggtag agcgtctgcg ggtcgcccag cacctcgagc
    14461 ttcaccagat cgtgcccgtc gaggagctcg cgcgcgaggc gcagtgtgcg caccgcgtcc
    14521 gccgcgttgt agcagcccgc cgtgttcggc aggtaggtga atttcgaggg cggcagcgcg
    14581 tcgagcaggc tcggctcgtt cgcgttctgg ccgatgttgg tgcgccggat cgccacggtc
    14641 acgatcgccg cgcccgaggc ctcgaccgcg gcgcgcgtct gcgcaaagtc gcgatacttg
    14701 ccggtgccga ccaagagccg ggagccgtag ctctttcctg cgatgttcaa agagtctttc
    14761 aagatcaacc cccgccaacc gccacgacaa tttcaagccg gtcgccgtcg gccagtacgg
    14821 tgctcgcgtg cgcgctcttc ggcacgatct ctccgttgcg ctccactgcc actttcttcc
    14881 ccaccagctc gagcttctcc agcagcccca gcacctgcaa gggcttttcc agccgatgcg
    14941 cagtgccgtt gacggttacc tcgatcatgg ccgcagatga taattccgat cgcgcacctc
    15001 gcggtattcg ccttcgatca cctgcccgcg gcgctccggc actcgtcccc gcgagcgcca
    15061 ccatacccag cccgcgagca acaggccggc ggtcagggcg atcgcaacca ggatcgaggt
    15121 gaacacgaat acggcgacca agaagagcac acccaggagg gcggaaagca cgcgctggat
    15181 catgggcgga ttatgcctcg ggctggtaga cgatagcccg gaccggagta gggttgtagt
    15241 cgttgaccgg attgttcatc tgcg
//
LOCUS       DATBSZ010000243         8746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_387192, whole genome shotgun sequence.
ACCESSION   DATBSZ010000243 DATBSZ010000000
VERSION     DATBSZ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387192"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(402..1268)
                     /locus_tag="VIG70_15430"
     CDS             complement(402..1268)
                     /locus_tag="VIG70_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8251992.1"
                     /translation="MAAVVFQELEISPQRRAWRRLFRRGGAMVGLAVVTFFVLIAIFA
                     PWLAPHDPLGTSWSAVRAAPSATYLFGADELGRDVLSRIIWGARASILAGVVSVSISL
                     ALGVPIGMAAGYLGRWIDALISRITDAMLACPFLILAIALAAFLGPSLTNAMIAIGIS
                     ATPIFIRLTRAQVLAAKSEDYVEAARALGNPHLRIALRHIFPNIVAPLIVQATLAIAA
                     AVIAEASLSFLGLGQQPPAPSWGSMLNTARNYVDQAPWMAIWPGVSIFLLVLSFNLLG
                     DGLRDALDPRHK"
     gene            complement(1268..2209)
                     /locus_tag="VIG70_15435"
     CDS             complement(1268..2209)
                     /locus_tag="VIG70_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8251993.1"
                     /translation="MLNYLLQRLATIVPTLFFVSVLIFGLQQLLPGDPAIALAGEERD
                     PETIRYLREKFHLDEPLPARYWYWLSGVMQGDLGDSVRIQKPVTELILQKLPVTIQLA
                     AMAMVIALLIGITAGVISAVKKDTWWDYGANIFALWGLSTPNFWLGILLILLFAVQLG
                     WLPASGYVSPFEDLKANLAAMIMPAFVLGNAIAAVLMRHTRSAMLQVLSSDYVRTARA
                     KGLDERVVVLKHALRNALIPVITLGALEFGTLLSGAVLTEQVFTIPGFGKLIVDAVFN
                     RDYLVVQGVVLFTATVYITLNLIADLAYFVVNPRMRH"
     gene            complement(2211..3707)
                     /locus_tag="VIG70_15440"
     CDS             complement(2211..3707)
                     /locus_tag="VIG70_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8251994.1"
                     /translation="MKTVLLAALFAFSGLAQSQTLRIGLAEDPDVLDPSMARTFVGRI
                     VFAGLCDKLFDLDEKLNVVPQLATSYKWSADNKSLEIKLRDGVTFHDGEKMDAQAVKY
                     NLERHKDMKGSNRRGELAVVSSVDAVDAATVRINLSAPFAPLLAVLTDRSGMMVSPKA
                     AEASGDKFGAKPVCSGPFRFVERIAQDRIVLERFPNYWNKGQIHFERIVYQPIVDATV
                     RLANLRSGQLDFIERVAPSDVPNLRNDSRFKLAKIVEIGYQGITINVGKSDLAQKNPL
                     GKDPRVREAFELSLDRDAIVKVAMEGEAQAGNQWVAPSNRFYGKSAPLPKRNVARAKQ
                     LLKEAGVPNPSFTLMTPTTSDAQRIAQIVQAMAKEAGFDVKIQSTEFATSLNLADKGQ
                     FEAYVLAWSGRADPDGNLHTMLACKGPTNYAGYCKEDVESLINTSRTSLDPGVRAAAY
                     DKIAQQVQKDRPIVYLFHRHWLWAHNAKLSGLRTVPDGMVRVQGLKMN"
     gene            complement(3719..4720)
                     /locus_tag="VIG70_15445"
     CDS             complement(3719..4720)
                     /locus_tag="VIG70_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY8251995.1"
                     /translation="MVKHFPVRTGLFGRARGAIQAVDDISFELYPGETLALVGESGCG
                     KSTAGKLLLRLLDPTAGNVFFRGKNILGLEEREMRSLRREMQIIFQDPYGSLNPRMTV
                     GDMLEEPLRLHGLHTGKERERTKELLGLVGLAPDHAQRYPHEFSGGQRQRIGIARALA
                     VEPRLIVCDEPVSALDVSIQAQVINLLQDLQRRFGLVYVFIAHDLAVVKHIATRVAVM
                     YLGKIVELADKKALFAAPRHPYTRALLSAIPVPDPSLKKGRTVLQGDVPSPYNPPSGC
                     RFRTRCPYVRPLCSTQVPVLENGVACHFWKEIEPFAAASQLTAVNERLLRLQAAFRS"
     gene            complement(4747..5697)
                     /locus_tag="VIG70_15450"
     CDS             complement(4747..5697)
                     /locus_tag="VIG70_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8251996.1"
                     /translation="MRGLRTHFASERGEVRAVDGVDLSLARGRTLGIVGESGCGKSVT
                     ALSIMGLVPQPPGRIAGGEVLFEGEDLLKLPARRMRDLRGDQLSMIFQEPMTSLNPAF
                     PVGEQVAEALLRHKDVSQNEAEDSAVEMLRRVRISSPERRALDYPHQLSGGMRQRVMI
                     AMALACNPKLLIADEPTTALDVTIQAQILELMRALRAELGTAIVLITHDLGVIAELAD
                     DVIVMYAGKVIERCTAERLFSEPQHPYTVGLLGSIPRLHLQQERLSAIEGFVPDASAF
                     PEGCRFHPRCPFAVDKCRSENPSLIEIEAGHHAACWRAPL"
     gene            complement(5699..6400)
                     /locus_tag="VIG70_15455"
     CDS             complement(5699..6400)
                     /locus_tag="VIG70_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEY8251997.1"
                     /translation="MTWSIIARDASGAFGVAIATRFFAVGALCPHAESNVGALSTQAL
                     VNPYYGRHGLDLLRAGMPAPEVVKHLIAPDEGREHRQLHVIDIEGRIGQHTGSSCVGW
                     CGAAAGDGFSVAGNMLSSEKVIRETARAFEKSRKPFAERLIAALEAGEAAGGDKRGKQ
                     SAALLIHSTEAYAELNLRVDDHADPLKELRRLYVKAHERFVPYLKCSPSKERPWGILD
                     RDEIEKEIASHKKPS"
     gene            6418..7653
                     /locus_tag="VIG70_15460"
     CDS             6418..7653
                     /locus_tag="VIG70_15460"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8251998.1"
                     /translation="MYREQIARLFERSAFYRRKLSAAGFDSPAAVGGLERLDSLPFTE
                     KDELRASQAESPPLGGHAAVDIAEVARIYSTSGTSGTPLYIPLTRNDVEDWRAIGRDT
                     YSKNGLKAGDSVVTTFGAGPFVAGASLAAFEAIGCTHIPVGVGNTERLLAALRHLKPK
                     CLGATPSYALHVAEKLGGMKSSLERVIVGGEPGGGEDGFRKKLQDALGVRVYEIMGIG
                     DVAASLWGECPEQAGMHFSGGGSIHVELVDPDSGKPVELKDGAKGELVYTHLRREAAP
                     VLRFRSRDHVQVWVSECSCKRRTMRVRCIGRTDDMLIVRGVNVFPSAIREIVGKFQPR
                     VTGVVLVKPRRRGVRQDPPLPVVVEGEVADHQLAGAIQEQIRNTLLVTTEIRLVAARS
                     LPRSEYKSKLIDYSEAEGG"
     gene            7655..8326
                     /locus_tag="VIG70_15465"
     CDS             7655..8326
                     /locus_tag="VIG70_15465"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY8251999.1"
                     /translation="MKLYYHPVSTTSRPVLLFIQENNLKVDTQVVDLMKGEHMQPAYA
                     AMNPSKLVPMLEDGDFRLTESSAILKYLAEKFELPSYPKDLRTRARVNERMDWINTQL
                     CRDLAYGLVYPQIFPFHKRPSDAHQKGQLAWAQERAKGWMKILDESLIGKQQYLCGDQ
                     ITIADYFASSFVSLAEFIRADFGPYPNLKRWYERMKGLKTWNKVHEVFYGFAGSMKDA
                     KFEVV"
     gene            8329..>8746
                     /locus_tag="VIG70_15470"
     CDS             8329..>8746
                     /locus_tag="VIG70_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8252000.1"
                     /translation="MIKKLHHNAYRCRDSEETRAFYEDFLGLKLACTLDIEETMTGRK
                     TRTLHTFYEMGDGSYLAFFEAPDRPFEFKDQHDYDLHIALEVEEPALAQMLAKGRAAG
                     IETRGITDHGFIHSIYLRDPNGYVIELTAKTPGHAEA"
BASE COUNT         1530 a         2838 c         2941 g         1437 t
ORIGIN      
        1 gcgctcccgc ggtcgaggcg tacccgcgcg cgggcgcgca gcgcacgaag gacgacaacg
       61 cctacttcgg caccgagccg cttttccgcc gagccgggtt ccgggtcatc cgaaaaccgg
      121 agaagctgcg aagaaactcg gtccagcgag tgacgatgcg caagctcgcg tgatgcgatt
      181 gctcgcggtg ttctgcgcgg taagcgtgct ccacttcgtg ttgagcgtcg ccggcatcgt
      241 actcgcactg ccagcggctt tcgacgcagc gccggcaaaa gtcatcctgg cctggaccgc
      301 agccgtgttg ctatcgccgc tggcattggt ctcaccggcc gactatgggt acccagagat
      361 cgccgcggtg agcgtgctgt tcggcctcgc ggccgctgca gttacttatg ccgtgggtcg
      421 agcgcgtcgc gcaggccgtc gcccagcagg ttgaaggaca gcaccagcag gaagatcgag
      481 acgcccggcc agatcgccat ccacggcgcc tggtcgacgt agttgcgcgc ggtgttgagc
      541 atgctgcccc agctcggcgc gggcggctgc tgtcccaagc ccaggaacga caggctcgcc
      601 tcggcgatca cggcggccgc gatggcgagc gtcgcctgga cgatgagcgg cgcgacgatg
      661 ttcgggaaga tgtggcgcag cgcgatgcgc aggtgcgggt tgccgagcgc gcgcgcggcc
      721 tcgacgtagt cctcggactt cgccgcgagc acctgcgcgc gggtcaaacg gatgaagatc
      781 ggcgttgccg agatgccgat cgcgatcatg gcgttggtga ggctcgggcc gaggaacgcg
      841 gcgagcgcga tggcgaggat caggaagggg caggcgagca tcgcgtcggt gatgcgcgag
      901 atcagcgcgt cgatccagcg gccgaggtag cccgcggcca tgccgatcgg caccccgagc
      961 gcgagcgaga tcgacaccga gaccacgccg gcgagaatgg aggcgcgcgc gccccagatg
     1021 atgcgcgaca gcacgtcgcg gccgagctcg tcggcgccga agaggtaggt ggcgctcggc
     1081 gcggcgcgca ccgcgctcca gctcgtgccg agcggatcgt gcggcgcgag ccacggcgcg
     1141 aagatcgcaa tcagtacaaa aaaggtgacg actgctaggc cgaccatggc gccgccgcgg
     1201 cgaaacaggc gtcgccaggc tcttcgttgg ggagagattt ccagctcttg aaagacgacg
     1261 gcggccatca gtgccgcatc cgcggattga cgacgaaata agccaggtcg gcgatcaggt
     1321 tgagcgtgat gtagaccgtg gcggtgaaga ggaccacgcc ctgcaccacg aggtagtcgc
     1381 ggttgaacac cgcgtcgacg atgagcttgc cgaagccggg aatggtgaag acctgctcgg
     1441 tgagcaccgc gcctgaaaga agcgtgccga actcgagcgc cccgagcgtg atgaccggga
     1501 tcagcgcgtt gcgcagcgca tgcttgagca ccacgacgcg ctcgtccagc cccttcgctc
     1561 ttgccgttct tacgtagtcg gaagaaagga cttgcagcat ggcgctgcgc gtgtggcgca
     1621 tcagcaccgc ggcgatggcg ttgccgagca cgaacgccgg catgatcatc gccgccaggt
     1681 ttgccttcaa atcctcaaac gggctgacgt agcccgaggc cggcagccag ccgagctgca
     1741 ccgcaaacaa caggatgagc aggatgccga gccagaagtt gggcgtggac aggccccaga
     1801 gcgcgaatat gttggcgccg tagtcccacc acgtgtcctt cttcaccgcg gagatcacgc
     1861 cggcagtgat gccgatgagg agggcgatga ccatcgccat ggcggcgagc tggatcgtga
     1921 ccgggagctt ttgcaggatc aactcggtga ccggcttctg gatgcgcacc gagtcgccga
     1981 ggtcgccctg catgacaccc gaaagccaat accagtagcg cgccggtagc ggctcgtcca
     2041 gatgaaactt ttctcttaga taccggattg tttccggatc gcgctcttct cccgcgagcg
     2101 cgatcgccgg gtcgccggga agaagctgct gcagcccgaa gatcaggacc gagacgaaga
     2161 acagcgtcgg aacgatcgtc gcgaggcgct gcagcaggta attcaacatc ctagttcatc
     2221 ttcaaaccct gcactcgcac catgccatcg ggcaccgtgc gcaggccgct gagctttgcg
     2281 ttgtgcgccc agagccagtg gcggtggaag agatacacga tcggccggtc cttctgcacc
     2341 tgctgtgcga tcttgtcgta ggccgctgcg cgcactcccg ggtcgagcga ggtgcgcgag
     2401 gtgttgatca gcgactcgac gtcctccttg cagtagccgg cgtagttggt cggccctttg
     2461 caggcgagca tggtatgcag gttgccgtcg ggatcggcgc gcccgctcca cgccagcacg
     2521 taggcctcga actggccctt gtcggcgagg ttgagcgagg tagcgaactc ggtcgactga
     2581 atcttgacgt cgaagccggc ctctttcgcc atcgcctgga cgatctgagc aatgcgctgc
     2641 gcgtcggaag tggtcggcgt catcagcgtg aagctcgggt tcggcacccc ggcttccttg
     2701 aggagctgct tcgcgcgcgc cacgttgcgt ttcggtagcg gtgcgctctt tccgtagaag
     2761 cggttcgacg gcgcgaccca ctggttgccg gcctgcgcct cgccttccat cgccaccttg
     2821 acgatcgcgt ctcggtcgag cgagagctcg aacgcctcgc gcacgcgcgg atccttgccg
     2881 agcgggttct tctgcgccag gtcgctcttg ccgacgttga tcgtgatgcc ctggtacccg
     2941 atctcgacga tcttggcgag cttgaagcga ctgtcgttcc tgaggttcgg cacgtcggag
     3001 ggcgcgacgc gctcgatgaa atcgagctgg cccgagcgca ggttggcgag gcgcaccgtg
     3061 gcgtcgacga tcggctggta cacgatgcgc tcgaagtgga tctggccctt gttccagtag
     3121 ttcgggaagc gctcgagcac gatgcggtcc tgcgcgatgc gctcgacgaa gcggaacggg
     3181 cccgagcaca ccggctttgc gccgaattta tcgccgctgg cctctgcagc cttcggcgac
     3241 accatcatcc cggagcggtc ggtgagcacc gcgagcagcg gcgcgaacgg cgccgacagg
     3301 ttgatgcgca ccgtggcggc gtccaccgca tccacgctcg agacgaccgc cagctcgccg
     3361 cggcggttcg agcccttcat gtccttgtgg cgctcgaggt tgtacttgac cgcctgcgcg
     3421 tccatcttct cgccgtcgtg gaaggtgacg ccgtcgcgca gcttgatctc gagcgacttg
     3481 ttgtcggccg accacttgta ggacgtggcg agctgcggga cgacgttcag cttctcgtcg
     3541 aggtcgaaga gcttgtcgca cagccccgcg aacacgatcc ggccgacgaa ggtgcgcgcc
     3601 atcgacgggt cgaggacgtc gggatcctcg gcgaggccga tgcggagggt ttggctctga
     3661 gcgagccccg agaatgcgaa cagcgcggcg agcaagacgg ttttcatgcg atacctcctc
     3721 aagatcggaa cgctgcctga aggcgcagca gtctctcatt gactgccgtc aattgggaag
     3781 ccgccgcaaa gggctctatc tctttccaga agtggcaggc tacgccgttt tcaagaaccg
     3841 gaacctgtgt actgcaaaga ggacgaacgt aggggcagcg ggtgcgaaag cggcagcccg
     3901 aaggcgggtt gtagggactg gggacgtcgc cctgcagcac ggtgcggcct ttcttcaacg
     3961 aggggtccgg caccggaatc gcggagagca gggcgcgggt atagggatgg cgcggcgcgg
     4021 caaagagggc cttcttgtcc gccagctcga cgatcttgcc gaggtacatc actgcgacgc
     4081 gcgtcgcgat gtgcttcacc actgcgaggt cgtgggcgat gaacacgtaa acgaggccga
     4141 accggcgctg caggtcctga agcaggttga tcacctgcgc ctggatcgag acgtcgagcg
     4201 ccgacaccgg ctcgtcgcac acgatgaggc gcggctcgac cgcgagcgcg cgcgcgatgc
     4261 cgatgcgctg gcgctggccg ccggagaatt cgtgcgggta gcgctgggcg tggtcgggcg
     4321 cgaggccgac gagcccgagg agctcttttg ttctttcacg ttcttttcct gtgtgcaacc
     4381 catgcagccg cagcggctct tccagcatgt cccccacggt catgcgcggg ttgagcgagc
     4441 cgtaggggtc ctggaagatg atctgcatct cccgccgcag cgagcgcatt tcccgctctt
     4501 ccaagcccag gatgttcttc ccgcggaaaa agacgttgcc cgcggtggga tcgagcaggc
     4561 gcaggagcag ctttcccgcg gtcgacttgc cgcagcccga ctcgcccacc agcgccagcg
     4621 tctcgccggg gtagagctcg aagctgatgt cgtccaccgc ctggatcgcg ccgcgcgcgc
     4681 ggccgaagag cccggtgcgg accgggaagt gcttcaccag cctgtccgcc ctcaggaggg
     4741 gctcgctcac agcggcgccc gccagcaggc ggcgtgatgg cccgcctcga tctcgatgag
     4801 agagggattt tcagaacgac acttgtcgac cgcgaacggg caacgggggt ggaagcggca
     4861 gccctccggg aacgccgacg catcgggcac gaagccctcg atcgcactca ggcgctcctg
     4921 ctgcaagtgc aggcgcggga tcgagccgag gaggcccacg gtgtacgggt gctgcggctc
     4981 ggagaaaagg cgctccgccg tgcagcgctc gatcaccttt ccggcgtaca tcacgatcac
     5041 gtcgtcggcg agctcggcga tcacgccaag gtcgtgcgtg atgaggacga tcgcggtgcc
     5101 gagttctgcg cgcagcgcgc gcatcagctc caggatctgc gcctggatgg tgacgtcgag
     5161 ggcggtggtg ggctcgtccg cgatcagcag cttcgggttg caggcgagcg ccatcgcgat
     5221 catcacgcgc tgccgcatgc cgcccgagag ctggtgcggg tagtcgagcg cgcggcgctc
     5281 gggcgaagag atgcgcacgc ggcgcagcat ttcgaccgcg ctatcttctg cctcgttttg
     5341 tgagacatcc ttgtgtctta acaatgcttc cgccacctgc tcgcccacgg ggaacgccgg
     5401 gttgagcgag gtcatcggct cctggaagat catcgagagc tggtcgccgc gcaggtcgcg
     5461 catgcgccgc gcgggcagct tgagcaggtc ctcgccctcg aacagcacct cgccgccggc
     5521 gatgcggccc ggcggctgcg gcacgaggcc catgatcgaa agcgcggtga cgctcttgcc
     5581 gcagcccgac tcgccgacga tgccgagcgt gcgaccgcgc gcgaggctca ggtcgacgcc
     5641 gtcgaccgcg cgcacctcac cgcgctcgga ggcgaagtgc gtgcgcagcc cccgcacctc
     5701 aagaaggttt cttatggctc gcgatctcct tctcgatctc gtcgcgatcg aggattcccc
     5761 acggccgctc cttgctcggg ctgcacttga ggtagggcac gaagcgctcg tgcgccttta
     5821 cgtaaaggcg cctcagctcc ttgagcgggt cggcatggtc gtcgactctc aggttgagct
     5881 cggcatacgc ttcggtcgaa tggatgagga gcgccgccga ttgcttgccg cgcttgtcgc
     5941 cgccagcggc ttcacccgct tcgagcgccg cgatcagccg ttcggcgaaa ggcttcctgg
     6001 atttctcgaa agccctggcc gtttcacgaa tgactttctc gctggaaagc atgtttccgg
     6061 ccacggaaaa cccgtctccc gcggccgcgc cgcaccagcc gacgcagctc gagccggtgt
     6121 gctggccgat gcgtccttcg atatcgatga cgtggagctg gcggtgctcg cggccctcgt
     6181 cgggcgcgat caggtgcttg accacctcgg gcgcgggcat cccggcgcgc aggaggtcga
     6241 gcccatgcct gccgtagtac gggttgacca gcgcctgggt ggagagcgcg ccgacgttgc
     6301 tctccgcgtg cggacagagc gcgcccaccg cgaagaagcg agtcgcgatc gccacgccga
     6361 acgcgccgct cgcatcacgc gctatgatcg accaggtcat gggcggcgat cataaccttg
     6421 tatagggaac aaatcgcgag gctgttcgag cgctcggcgt tctatcgccg caagctgagc
     6481 gccgccggat tcgactcgcc cgcggcggtc ggcggcctcg aacgcttgga ctcgctgccc
     6541 ttcaccgaga aggacgagtt gcgcgcgagc caggcggagt cgccgccgct cggcggccat
     6601 gccgcggtcg acatcgccga ggtggcgcgc atctactcga cgagcggcac cagcggcacg
     6661 cctctctaca tcccgctgac caggaacgac gtcgaggact ggcgcgcgat cggccgcgac
     6721 acctactcga agaacggcct taaggcgggc gacagcgtcg tcacgacctt cggcgccggg
     6781 ccgttcgtcg ccggagcgtc gctcgcggcc ttcgaagcca tcggctgcac ccacattccg
     6841 gtcggcgtcg gcaacacgga aaggctgctc gccgcgctgc gacacctcaa gccgaagtgc
     6901 ctcggggcga cgccttccta tgcgctgcat gtggccgaga agctcggcgg catgaagagc
     6961 agcctggagc gcgttatcgt cggcggcgag ccgggcggag gcgaggacgg cttccggaag
     7021 aagctgcagg acgcgctggg cgtgcgcgtc tacgagatca tgggcatcgg cgacgtcgcg
     7081 gcctcgctct ggggcgaatg cccggagcaa gcaggcatgc acttctccgg cggcggcagt
     7141 atccacgtcg agctggtcga ccccgattcc ggcaagccgg tggagctcaa ggacggagcg
     7201 aagggcgagc tggtctacac gcacctgcgg cgcgaggccg cgccagtgtt gcgtttccgc
     7261 agccgcgacc acgtgcaggt ctgggtttca gaatgttcct gcaagagaag aaccatgaga
     7321 gtcaggtgca tcggccgcac cgacgacatg ctgatcgtgc gcggcgtcaa tgtctttccc
     7381 tctgccatcc gcgagatcgt cggcaagttc cagccgcgcg tcaccggcgt cgtgctggtc
     7441 aagccgcgcc gccgcggcgt gcgccaggac ccgccgctgc cggtggtggt cgagggcgag
     7501 gttgccgacc atcagctcgc cggcgcgatt caggagcaga tccgcaacac cctgctcgtg
     7561 accaccgaga taaggctggt ggccgcaaga agcttgccgc gctccgaata caagtccaag
     7621 ctgatcgact attccgaagc cgaaggaggg tgacatgaag ctctactacc atcccgtctc
     7681 caccaccagc cgcccggtgc tgctgttcat ccaggagaac aacctcaagg tggacacgca
     7741 ggtagtcgac ctgatgaagg gcgagcacat gcagccggcg tacgccgcga tgaacccgag
     7801 caagctcgtg ccgatgctcg aggacggcga cttcaggctc accgagagct ccgcgatcct
     7861 caagtacctc gccgagaagt tcgagctgcc ttcgtacccg aaggacctgc gcacccgcgc
     7921 gcgcgtcaac gagcgcatgg actggatcaa cacccagctc tgccgcgatc tcgcgtacgg
     7981 cctggtgtac ccgcagatct tcccgttcca caagcggccg agcgacgcgc accagaaagg
     8041 ccagctcgcc tgggcgcagg agcgcgcgaa gggctggatg aagatcctcg acgagagcct
     8101 gatcggaaaa cagcagtatc tctgcggcga ccagatcacc atcgccgact acttcgcttc
     8161 ctccttcgtc agcctggcgg aattcatccg cgccgatttc ggaccgtacc cgaacctcaa
     8221 gcgctggtac gagcgcatga agggcctcaa gacctggaac aaggtgcacg aggtgttcta
     8281 cgggttcgcc ggctcgatga aggacgccaa gttcgaagtg gtgtgaccat gatcaagaaa
     8341 ctgcatcaca acgcctaccg ctgccgcgat tcggaagaga cgcgcgcctt ctacgaggac
     8401 ttcctcggcc tcaagctcgc ctgcacgctc gacatcgagg agaccatgac cgggcgcaag
     8461 acccgcactc tccacacctt ctacgagatg ggcgatggtt cctacctcgc gttcttcgag
     8521 gcgcccgacc ggccgttcga gttcaaggac cagcacgact acgacctgca catcgcgctc
     8581 gaggtggaag agcccgcgct cgcccagatg ctcgccaagg gccgcgcggc cggcatcgag
     8641 acgcgcggca tcaccgacca cggcttcatc cactcgatct acctgcgcga ccccaacggc
     8701 tacgtcatcg agctcacggc gaaaacgccg ggccacgccg aggcga
//
LOCUS       DATBSZ010000244        24433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1820872, whole genome shotgun sequence.
ACCESSION   DATBSZ010000244 DATBSZ010000000
VERSION     DATBSZ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 24433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24433
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820872"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VIG70_15475"
     CDS             <1..358
                     /locus_tag="VIG70_15475"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEY8252001.1"
                     /translation="KMLGDLAEILQVEDLLHCSFVPLRNIARTTMPEEKFHAQFGLDF
                     CVDLVETEQGRARVQKAVNGFYPEVPKFFGASGSRNNQLYRQFGIKLRSNEEMRADYM
                     ARARSTVEPLGITLPA"
     gene            355..831
                     /locus_tag="VIG70_15480"
     CDS             355..831
                     /locus_tag="VIG70_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252002.1"
                     /translation="MKEAWFSALGEPLGEHERGEIAAYLAGLGMDAAMPVREALTWKK
                     ADELCRTQSDDWWRAEEEERARLECGARLDPADPEWLALNDELHGAAAVAAARAGCAD
                     AALIRVAAGAASHAAYQARLARAAGAPSGHPFLRKYALYCGGRWPLGIYDQSFAIF"
     gene            835..1701
                     /locus_tag="VIG70_15485"
     CDS             835..1701
                     /locus_tag="VIG70_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8252003.1"
                     /translation="MRICRFDNDRLGIVEGGAVLDVSEALAAIAPQQWPYRHGDALIA
                     NLPAVLERAKALAPQAPRRKLGEIRLRPPVANPSKIVNAPINYQAHIDEAKRDQGIAH
                     GRDIKTIADWGLFLKSPSSLVGAGDAIALRFPERRNDHEVELAVVIGREANRVPRASA
                     LDYVCGYSIGLDMTLRGPEFPSFRKSIDTYSVLAPWLVTRDEVPDPNALELWLTVNGE
                     ARQRSNTRRMVYDVARLIEYATSFYTLHPGDLIFSGTPEGVGPVKPGDEIRAGIERIG
                     EFTIRVAPDYAE"
     gene            1698..3149
                     /locus_tag="VIG70_15490"
     CDS             1698..3149
                     /locus_tag="VIG70_15490"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8252004.1"
                     /translation="MNLAEHVLRNRFRGRIALIGGGEQVSWDALADRVARVAGALRSH
                     GVRPGEQVLFVMRDTPAFAAAWLGAVHAGAIAVALNSKLADADYRHILVDSRAKLAMV
                     DEQFTAARDVFGKICVELEPGSPVPPFDAEPDAPALCLYSSGTTGRPKGILHAHRAAL
                     SIGEAFRALGIGEGDRVFTTSKFFFAYGLEHALLAPLAMGVTSILSADWPDADAVLGI
                     VERHRPKAIFSVPTIYRRLLAEPARRLEPLREVRHFVAGGERLSPQLVQQWRSAAGGE
                     LLNLYGMSEGFCACMMTPPGTSDGLRTGKPFPIAEVRLADGDGAEPAPGEPGVLWLRH
                     PAQARGYANLPEQTRSQFRDGWFCSRDLFMRDAQGFYIHQGRTDELVKVAGQWVWPSE
                     LEEAALAAAAVADAACVPVPDGEGLQRLALFVTARNDEDPTRAAADACERLPRYKRPK
                     WIRAVPELPRTASGKVQRYKLREILERELGRSG"
     gene            3174..3866
                     /locus_tag="VIG70_15495"
     CDS             3174..3866
                     /locus_tag="VIG70_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8252005.1"
                     /translation="MGHAISLWIAPALPVAGTADSFPVRRIFCVGRNYAEHQKEMGGD
                     GREQPFFFIKSAHTLVAGGGEVHYPAKTSNYHYETELVVALAKGGRRIDAKRANDHVF
                     GYAVGLDMTRRDLQQWGKDHGRPWDFGKDFDEAAPCSALVSAAKIGHPARGAIWLTVN
                     GKQRQRADLGDMIWSVPEQIAYLSEYYTLEPGDLIYSGTPAGVGPVKPGDELHAGVDG
                     VGELKVKILPAL"
     gene            3863..4501
                     /gene="maiA"
                     /locus_tag="VIG70_15500"
     CDS             3863..4501
                     /gene="maiA"
                     /locus_tag="VIG70_15500"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEY8252006.1"
                     /translation="MKLYTYFRSSAAFRVRIALNLKGLPYEPVFVHLAKGEHRKPEYS
                     SINAQALVPTLEFDDGTRLNQSLAIIEYLEEKRPHPALLPADSLGKARVRALSDLIAC
                     EIHPLNNLRVLQHLKRVLGQSEDQVNAWYRHWIADGLAKFEAELASDKRGKFCYGDSP
                     TMADCCLVPQIFNAKRYDCDLNPYPSTVRAFEHCMKLDAFDRAQPAKQPDAE"
     gene            4506..4916
                     /gene="ligA"
                     /locus_tag="VIG70_15505"
     CDS             4506..4916
                     /gene="ligA"
                     /locus_tag="VIG70_15505"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HEY8252007.1"
                     /translation="MKPNEYDDIPGTTVFDAQRSRQGYHLNMFCMSLMKAENRAAFKA
                     SERAYLDRYPMTEEQKQAVLDRDWNRMISLGGNIYFLAKLFFTDGKSFQYVAAVMTGS
                     TQEEYAQMMLNGGRPPEGNRYVAEWEKQPDRKRG"
     gene            4909..5751
                     /locus_tag="VIG70_15510"
     CDS             4909..5751
                     /locus_tag="VIG70_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HEY8252008.1"
                     /translation="MARIVAGVATSHVPAIAAALDLGKSGEPYWKRVFDGYQRSKEWL
                     RKVDPDVIILVYNDHASAFSLEIIPTFALGCAAQFPIADEGWGPRPVPAVKGHPELAA
                     HIAQSLILDEFDLTIVNRMDVDHGLTVPLSLMYGQPKEWPCPVIPLAVNVVQYPPPTG
                     RRCYHLGRAIRKAVESFDQNLRVVVFGTGGMSHQLQGPRAGLINKAFDARFLDAIARD
                     PAALTRISHVEYLREAGSEGIELVMWLVMRGALDDEVEEVHRFYHVPASNTALGHIIL
                     ENRK"
     gene            5748..6695
                     /locus_tag="VIG70_15515"
     CDS             5748..6695
                     /locus_tag="VIG70_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8252009.1"
                     /translation="MKICVAGQGAFGQKHLEGIANIKGVEVVSLAGGNPEATAEVARK
                     FKIPHATGDLAQSLKQPGVEAVILATPTQMHARQAIQCLRAGKHVQVEIPMADTLADA
                     REIVKVQKETGLVAMAGHTRRFNPSHQWVRKKIVAGELRLLHLVVQTFFFRRTNMNAA
                     GKPRSWTDHLLWHHACHTVDLFQYQAREPIAKARAVQGPMHPTLGIAMDMSIQMTTPS
                     GVACSLALSFNNDGPLGTFFRYICDNGTYIARYDDLFDGKDNKIDVSKVDVSMNGIEL
                     QDREFFAAIREKREPNASVAQCLPAMEALDRLEKSLSAP"
     gene            complement(6699..7130)
                     /locus_tag="VIG70_15520"
     CDS             complement(6699..7130)
                     /locus_tag="VIG70_15520"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY8252010.1"
                     /translation="MSEFESIVGHFLCEVHWGDCDPAGIIFYPTYFRWMDAATWAFMG
                     SVGYGPKRMRDEHLAMPLVGADCQFLAPAQHGDRCEVRSRIARFGGASFVVAHDVLRS
                     DGTTLAKGSEKRVWGRYVNGPGTPLKGQQIPDDVRALFLAR"
     regulatory      7178..7255
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            7294..10977
                     /gene="metH"
                     /locus_tag="VIG70_15525"
     CDS             7294..10977
                     /gene="metH"
                     /locus_tag="VIG70_15525"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY8252011.1"
                     /translation="MDKLNTLLRERILLLDGAMGTMIQRARLAEEDYRGARFRDWPRE
                     LRGNNDLLSLTQPRLVRDIHAQYLQAGADVIETNTFNSTSIALADYGMEALAREFNSA
                     AARLATDAAREWASRTPKQPRFVAGVLGPTNKTASISPDVNDPGFRAVSFDELVTAYA
                     EAIDGLLEGGVDLLLVETIFDTLNAKAALFAIEQCFAQSGTRLPVIISGTITDASGRT
                     LSGQTAEAFYNSVRHARPLAVGLNCALGAKELRPYVEELAGIAESYVSCHPNAGLPNA
                     FGEYEDTPESMAAQAAEWARAGWLNLAGGCCGTTPEHIRAIGEALRPIPPRKIPDRPR
                     ALRLAGLEPLNVDDRSLFVNIGERTNVTGSKAFARLVLAGDYAGALAVARQQVENGAQ
                     VIDVNMDEAMLDSEKAMSTFLRLVAAEPDISRVPVMIDSSKWSVIEAGLKCVQGKPIV
                     NSISMKEGEETFLHHAALCRRYGAAVVVMAFDEKGQADTLERRTAVSRRAYDLLVNKA
                     GFLPEDIIFDPNIFAVATGLEEHNRYAIDFIEAVRWIRTNLPQTRVSGGVSNLSFSFR
                     GNDGVREAMHTAFLYHAIRAGLSMGIVNAGQLGVYEQIEPELRLRVEDVLFDRRPDAT
                     ERLVEFAATVKTGARETAKDEAWRTGSVEQRLAHALVNGISSHIVADTEEARRKSARP
                     IEVIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAHLVPYIEAEKAKSGEAG
                     RAKGRLVLATVKGDVHDIGKNIVAVVLQCNNYEVVNLGVMVPAETILETARREKADVI
                     GLSGLITPSLEEMAHVAREMERTQFKLPLLIGGATTSRAHTAVKIAPNYSGPVVYVPD
                     ASRSVPVVQNLLSQKPQAYLAEVRADYERIRIQHRDKKGPGPLHPLARARELGFKVDW
                     RKYLPPAPAKAGLTALRRYPLAELVDHIDWGPFFQAWELSGPYPKILEDPVVGEAARK
                     LFAEAQETLARIVRENLVVANGVFALYPAARVDHDDIEIYADEERRKPLMTWHNLRQQ
                     NHKPEGRANLCLADFVAPKASGVRDWVGAFAVSAGGIEERVAELEKRGDDYNAIMLKV
                     LADRLAEAFAERLHERVRREFWGYAAEERLSGAELIAERYRGIRPAPGYPACPDHTAK
                     GPLFRLLDAEKNADMKLTESFAMLPASAVSGFYLSHPEASYFAVGKIGRDQVEDYARR
                     SRMSMAEAEKWLAPYLAYELETV"
     gene            10986..11087
                     /locus_tag="VIG70_15530"
     CDS             10986..11087
                     /locus_tag="VIG70_15530"
                     /inference="COORDINATES: protein motif:HMM:NF028380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HEY8252012.1"
                     /translation="MGKGDKRTRRGKIYKGSYGKKRPHRQKPKPKKT"
     gene            complement(11088..11273)
                     /locus_tag="VIG70_15535"
                     /pseudo
     CDS             complement(11088..11273)
                     /locus_tag="VIG70_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299529.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(11592..11945)
                     /locus_tag="VIG70_15540"
                     /pseudo
     CDS             complement(11592..11945)
                     /locus_tag="VIG70_15540"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11988..12335)
                     /locus_tag="VIG70_15545"
                     /pseudo
     CDS             complement(11988..12335)
                     /locus_tag="VIG70_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(12340..14406)
                     /locus_tag="VIG70_15550"
     CDS             complement(12340..14406)
                     /locus_tag="VIG70_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8252013.1"
                     /translation="MKWLILVGGAVAAVGLFLLATASADTTIFARHYPLLLGLNAALA
                     ALLAALVGIQLVMIARRHRARVFGARLTLRLLARFAALAVVPGLIVYAVSVQFLTRSI
                     ESWFDVKVDAALEGGIKLGQQAIEQMTIDLQGKANAMALELAERSPSELATQLERLRG
                     QLGVEEAVIVTGSGRLVASAAHDVTKLVPELPAGQALRQARAKRSHTEVDATVGRPLS
                     LRVIVPVDFPGEMRFLQLRQSVPASFAQSAEAVEAAYRDYRELAISREGLKRIYIVTL
                     TFALLMALFVAVAVAATQSGLLAEPLANLAQATQAVARGDFSRRAPVTSRDELGVLTE
                     SFNSMTRQLEEARRGVESNQLALEGAKARLENILANLSAGVLVFDHALELSISNHGAG
                     AILGPELDAFAAQMRAHFAEKGEADWQLELHLRGTGKTLHARGARLPLATGGGAVVVF
                     DDITQLVQAQRATAWAEVARRLAHEIKNPLTPIQLSAERLEMKLGARLPREDADTLKH
                     ATETIVNQVAALKAMVDDFREYARLPAPEPAPLDLNALVRQVLALYENSTVPIAKHLA
                     EPLPPVWADGAQIRQVIHNLVQNAQDALEQQAGRGASPAIEVRTELAGDRIRLAVSDN
                     GAGFPEELMARIFEPYVTTKPRGTGLGLAIVKKIVDEHHGAVTIENRPESGASVSVLL
                     PLAKAA"
     gene            complement(14403..14969)
                     /locus_tag="VIG70_15555"
     CDS             complement(14403..14969)
                     /locus_tag="VIG70_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEY8252014.1"
                     /translation="MRRIARVSIIIGGFLAMAIIAAAPRAEDIEVRDVALRSVEEGLA
                     LDADFAFELTPRLADVVGNGVPLYFRVDFELTRRRWYWFDDTAASQQLQLRLSYHALS
                     RQYRLSTGLLQQSFPTLEEALNVLKRVRNWLVVERAVRLADADYDAAVRMRLDTTLLP
                     KPFQLSALTGRDLQLESAWKRFILRAPR"
     gene            complement(14981..16249)
                     /gene="rsmB"
                     /locus_tag="VIG70_15560"
     CDS             complement(14981..16249)
                     /gene="rsmB"
                     /locus_tag="VIG70_15560"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEY8252015.1"
                     /translation="MIALATALRDAATIVARVAAGASLATEFERFADEGASTPRAALI
                     DLTHGTLRRYGRVQAIVRQLSRRERGDPVAEALLWCSLYALESGRYADYTVVDQAVRA
                     CAMLERWSAKGFVNALLRGYLRERTSLEARIRADAEARYQHPRWWIDLLKQCYPGGWT
                     ETLAAGNSHPPMCLRVNRRRSSVADYRAKLAAEGIAAREVGHEALLLERPVPVERLPG
                     FAAGEVSVQDSGAQRAARCLELAAGQRVLDACAAPGGKAGHVLETADVELTALELDAA
                     RRERLERNLARLGLGARVVNADCTRPGDWWDRVPFDRILADVPCSASGIARRHPDIKW
                     LRRSHDLAAFAARQAEILDALWQVLCPGGKLLYATCSVFPQENDAVIEGFLARAARAV
                     RVPLPDGAAAQGLPDAERDGFYYALIHKQD"
     gene            complement(16246..17166)
                     /gene="fmt"
                     /locus_tag="VIG70_15565"
     CDS             complement(16246..17166)
                     /gene="fmt"
                     /locus_tag="VIG70_15565"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY8252016.1"
                     /translation="MRLVFAGTPEFAERALARLAAAGHDIALVLTQPDRPAGRGLQPL
                     ASPVKRFAAARRIAVLQPPRLRGAPEVEAVRAMRPDALVVAAYGLLLPRELLDAGRHG
                     ALNIHASLLPRWRGAAPIQRALLAGDTETGVSIMLMDAGLDTGPILMQKRIAIAPDED
                     AGSLHDKLAVQGAEAIVAALDEIEAGRARPTPQAESGVSYAAKIGKRDTHLDWSRPAC
                     DLERAVRAFRPAPGAFALLDGEPVKLWRASVVDARGAPGTVLDSQESLVIACGERALA
                     VSELQRAGGRRLSAAEFVRGYRLAQGTRFE"
     gene            complement(17180..17683)
                     /gene="def"
                     /locus_tag="VIG70_15570"
     CDS             complement(17180..17683)
                     /gene="def"
                     /locus_tag="VIG70_15570"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY8252017.1"
                     /translation="MARLTILRYPDARLHKVAAPVTVFDDSLKKLVAEMAETMYAAPG
                     IGLAAIQVDVPKQVIVVDVSERRDSLVVLVNPEILEATGVSDIEEGCLSVPGIYELVE
                     RAERVKVRAYDQNGNPFTLEAQGLLAVCIQHEMDHLKGKVFVEHLSQLKQQRIRAKLA
                     KQLRKSA"
     gene            17816..19051
                     /locus_tag="VIG70_15575"
     CDS             17816..19051
                     /locus_tag="VIG70_15575"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY8252018.1"
                     /translation="MVQQACKSIIALAFLVPGALFAQAPSTPSAAPATSAPSPARSSA
                     PLAIKPDAPDRYTVVPGDTLWGISQRYTDSPWRWPELWNMNREQIRNPHLIYPGYVIV
                     LDRERAQLTIAAPGTERAPGTTPAPGVPPSTTTEPTTPSTTTTTTAGTAGTAGTAPGT
                     VRLGPRVRAESLAKQEIPSIPPSVIEPFLSRPLVVEPDGLEKAPTIIGTQADRVVLAA
                     GNAAYVRGMGTSKDENWHIYRRGVALVDPDTNKTLGYEAVYLGTAQLTRPGEPATVVL
                     TSAVQEVELGDKLVAAGRPQPVNYAPRAPAAQIKGRVIAIYGGVAQVGEAGPQQIISI
                     NRGKADGIEVGHVLALYSLGGTVRDATKPRSAANARIPLPDERAGLAFVFRVFDRISY
                     ALIMQITRPVSPADVVQTP"
     gene            19064..20176
                     /gene="dprA"
                     /locus_tag="VIG70_15580"
     CDS             19064..20176
                     /gene="dprA"
                     /locus_tag="VIG70_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY8252019.1"
                     /translation="MNSDPGLASWLQLALTPGLGAATLQRMLRQFGLPQAALARKRAE
                     LGAFTTPAVLAALDSEAVAHAVEQALAWASQPGHAVITLADDTYPRLLLEISDPPALL
                     YARGRTELLSRTALAIVGSRNATQQGEANAEALAKALSNAGLTIVSGLALGIDAAAHR
                     GGLAGAGSTIAVLGTGIDVVYPSRNAALAAEIAERGVLISEFPLGAAPAAQNFPRRNR
                     LISGLAQGCLVVEAALASGSLITARAAAEQGREVFAIPGSIHSPLAKGCHALIKSGAK
                     LVESAEDVLAELSGFRPSGYASTTASTAKAPPAAADAGLLAYMGHDPVDVDSLCARAG
                     MSAEQVSSELLRLELDGRVAALPGGLYQRLEKGQRR"
     gene            20215..20673
                     /locus_tag="VIG70_15585"
     CDS             20215..20673
                     /locus_tag="VIG70_15585"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEY8252020.1"
                     /translation="MYDILVFVFENCQQADVAYDRERVAKKLSAAGFEDSDISEALHW
                     LAGMARAPQGVREPLPDARSSFRAFAPRELMKLDARCRGFLLTLEQSGILSAETRELV
                     IERALAASGEALSLEQLKLVVLMVLWNQQTATSRLVAEDLFTAAHARLAS"
     gene            20739..23243
                     /locus_tag="VIG70_15590"
     CDS             20739..23243
                     /locus_tag="VIG70_15590"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF011313.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEY8252021.1"
                     /translation="MAKKLIIAEKPSVAADIARALGGFKRSGEYFESERYVLSSAVGH
                     LLTLAVPEEHDVKRGKWSFSRLPVIPPHFALAPIEKSETRLNLLLRLMKRKDVTGLVN
                     ACDAGREGELIFRYIVQHARNKKPIERLWLQSMTQQSIREGFEKLRADKELLPLADAA
                     KSRSEADWLVGINGTRAMTAFNSKEGGFYLTTVGRVQTPTLAILVEREERIRAFTARD
                     YWEVQARFGAKAGEYPARWFDPKFKKDDDTERKPERLWSEAEAKAIVAACRGKTGIAT
                     EESKPSTQLSPLLYDLTSLQREANSGFGFPARMTLSLAQALYERHKVLTYPRTDARAL
                     PEDYLATVKKTLDELGDTREFAPFAKQILKQGWVKPNKRIFDNSKISDHFAIIPTLQT
                     PKHLNEAEQKLYDMVVRRFLAVFYPAAEYLQTTRITRVVEHQFKTEGKVLETPGWLAV
                     YGRAGGEENENLPAIAQEEKVPVREIAAHANQTKPPPRYTEATLLSAMEGAGKLVEDD
                     ELREAMAAKGLGTPATRAATIEGLIREDYVHREGRELVPTPKAFSLMFALHMLHIVEL
                     GSPELTGEWEHKLKLIEAGKLTRAEFMDQISALVKTVVNTIKHGEIPDVVYATVPAPC
                     PKCGGAVQETYRKVECQKCDFSLWRVISGREWTPEELAELFKKRFVGPLTGFRSKQGR
                     PFSAGIRLTDELRLEFDFGQGAGDGDEAVDFSAQEPLGACPKCKSRVFEHGVAYVCEK
                     AVGPQRSCDFRSGRLILQQPIEREQMKKLLETGRTDLLTGFVSKKNRKFKAFLVKQPD
                     GKIGFEFQPRAPKEGKEKPVAPEGKATRRGSERRAR"
     gene            complement(23209..>24433)
                     /locus_tag="VIG70_15595"
     CDS             complement(23209..>24433)
                     /locus_tag="VIG70_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY8252022.1"
                     /translation="EMTRRKGVLDGMGLPGKLADCQERDPALSELYLVEGDSAGGSAK
                     QGRDRKFQAILPLKGKILNVERARFEKLLSSAEIATLITALGCGIGTEEYNPDKLRYH
                     RIIVMTDADVDGSHIRTLLLTFFYRQMRELVERGHIYIAQPPLYKAKLGKEERYLKDE
                     HELNAFMLKQALVDAALHPGAGREPISGDTLAELAKSYLLAEAVMERVSRLVDREVLQ
                     AMLRGVQVDLTRRDTAEASAEALEAAIGGEGFAVAPHFDQKTERYQVRVERTRHGNVR
                     MSTIDTEFVHSGDYAQIRQTAEMLRGLIGTGAAVQRGDKRQPVADFAEAMKWLLGEVE
                     KAMTLQRYKGLGEMNPPQLWETTMDPATRRLLKVQIEDSIVADEIFTKLMGDEVEPRR
                     AFIELNALGVRNLDV"
BASE COUNT         4148 a         8435 c         8166 g         3684 t
ORIGIN      
        1 caagatgctc ggcgacctcg ccgagatcct ccaggtcgag gacctgctgc actgctcctt
       61 cgtgccgctg cgcaacattg cgcgcaccac gatgccggag gagaagttcc acgcccagtt
      121 cggcctcgat ttctgcgtcg acctggtcga aaccgagcag ggccgcgcgc gggtgcagaa
      181 agcggtcaac ggcttctacc cggaagtgcc gaagttcttc ggcgcgagcg gctcgcgcaa
      241 caaccagctc taccgtcagt tcggcatcaa gctgcgctcc aacgaagaaa tgcgcgccga
      301 ctacatggcg cgcgcgcgca gcacggtcga gcccctcggc attacccttc ccgcatgaag
      361 gaggcctggt tcagcgccct aggcgagccg ctcggggagc atgagcgcgg cgagatcgcg
      421 gcctatctcg ccggattggg catggatgcc gccatgccgg tacgcgaagc gctgacctgg
      481 aagaaggccg acgagctgtg ccgcacgcag tcggacgact ggtggcgcgc cgaagaagaa
      541 gagcgcgctc gcctggagtg cggcgcgcgg ctcgatccgg cagacccgga atggcttgcg
      601 ctgaacgacg aattgcacgg cgcggcggcg gtggccgccg cccgggccgg gtgcgccgac
      661 gcagcgctga tccgcgtcgc cgccggtgcg gcgagccatg ccgcctacca ggcgcggctt
      721 gcccgcgccg ccggcgcccc gagcggccat ccgttcctgc gcaagtacgc cctctattgc
      781 ggcggacgct ggccgcttgg catctacgac caatccttcg cgatcttctg aacgatgaga
      841 atctgccgct tcgacaatga ccgcctcggg atcgtcgagg gcggcgctgt gctcgacgtg
      901 agcgaggccc tggcggcgat tgcgccgcag caatggccgt accgccacgg cgacgccctc
      961 atcgcgaatc ttcccgccgt tctggaaagg gcgaaggccc ttgcgccgca ggccccgcgc
     1021 cgcaagctgg gcgagatccg gttgcggccg ccggtggcga acccctccaa gatcgtcaat
     1081 gcgccgatca actaccaggc gcacatcgac gaggcgaagc gcgaccaggg catcgcgcac
     1141 gggcgcgaca tcaagaccat cgccgactgg ggactgttcc tcaagtcgcc cagctcgctg
     1201 gtcggcgcgg gcgacgccat cgccttgcgc ttccccgagc ggcgcaacga ccacgaggtc
     1261 gagctcgcgg tggtgatcgg acgcgaggcg aaccgcgtgc cgcgcgctag cgcgctcgac
     1321 tacgtctgcg gctattcgat cggcctcgac atgaccttgc gcggacccga gttcccgagc
     1381 tttcgcaagt cgatcgacac gtacagcgtg ctcgcgccct ggctggtcac ccgcgacgaa
     1441 gttcccgatc cgaacgcgct cgagctctgg ctcacggtca acggcgaggc gcggcagcgc
     1501 tccaatacgc gccgcatggt ctacgacgtg gcgcggctga tcgagtacgc gacgagcttc
     1561 tacacgctcc atcccggcga tctcatcttc agcggcacgc ccgagggcgt cggcccggtc
     1621 aagcccggcg acgagatccg cgccggcatc gagcgcatcg gcgaattcac catccgcgtg
     1681 gcgcccgact acgcagaatg aatctcgccg agcacgtgct gcgcaaccgg ttccgcggcc
     1741 gcatcgcctt gatcggcggc ggcgagcagg tgagctggga cgcgctcgcg gaccgggtgg
     1801 cgcgcgtggc aggcgcgctg cgctcgcacg gcgtgcggcc gggcgagcag gtgctgttcg
     1861 tgatgcgcga tacgccggcg ttcgccgcgg cgtggctcgg cgcggtgcac gccggcgcga
     1921 tcgcggtggc gctcaacagc aagctcgcgg acgccgatta ccggcatatc ctcgtcgaca
     1981 gccgcgccaa gctcgcgatg gtagacgaac agttcaccgc cgcgcgcgac gtgttcggga
     2041 agatctgcgt cgagctggag cccggcagcc cggtgccgcc tttcgacgcc gagcccgatg
     2101 cgccagcgct gtgcctttac tcctccggca cgacgggacg accgaaagga atcctgcatg
     2161 cgcaccgggc ggcgctcagc atcggagagg cgttccgggc gttgggcatc ggcgaaggcg
     2221 accgcgtgtt caccacgtcg aagttcttct tcgcctacgg gctcgagcac gcgctgctcg
     2281 cgccgcttgc gatgggcgtg acttcgatcc tgagcgccga ctggccggac gccgacgcgg
     2341 tgctcggcat cgtcgagcgc caccgcccga aggcgatctt cagcgtcccc acgatctacc
     2401 ggcgcctgct ggccgagcct gcgcggcgtc tcgagccttt gcgcgaggtg cgccacttcg
     2461 tggccggcgg cgagcgcctt tcgccgcagc tcgtccagca gtggcgttcg gccgccggcg
     2521 gcgagctgct caacctctac ggcatgtcgg aaggattctg cgcctgcatg atgacgccgc
     2581 cgggcacctc cgacgggctg cgcaccggca agccctttcc gatcgcggag gtgcgcctcg
     2641 ccgacggtga cggcgccgag ccggcgccgg gcgagccggg cgtcctctgg ctgcgccatc
     2701 ccgcccaggc gcgcggctac gccaaccttc cggagcagac gcgcagccag tttcgcgacg
     2761 gatggttctg ctcgcgcgat cttttcatgc gcgatgcgca aggcttctac atccaccagg
     2821 ggcgcaccga cgagctggtg aaggtcgccg gccaatgggt gtggccttcg gagctggagg
     2881 aagcggcgct tgcggctgcg gcggtagcgg atgcggcctg cgtcccggtg cccgacggcg
     2941 aagggctgca acgtctcgcg cttttcgtca ccgcccgcaa cgatgaagat cccacacgcg
     3001 cggccgcgga tgcctgcgag cgcttgccgc ggtacaaacg gccaaaatgg atccgcgcag
     3061 tgcccgagct gccgcgcacg gcgagcggca aggtgcagcg ttacaagctg cgcgaaatcc
     3121 tggagcgcga gctggggcgc agcggctaaa attctgtctt ttcgaggaac cccatgggcc
     3181 acgcaatttc gctatggatc gcgccggcgc tgccggtcgc gggtaccgcg gacagctttc
     3241 cggtccgacg catcttctgc gtcgggcgca actacgccga gcaccagaag gagatgggcg
     3301 gcgacgggcg cgagcagccg ttcttcttca tcaagagcgc gcacacgctg gtcgccggcg
     3361 gcggtgaggt gcactacccc gccaagactt ccaactacca ctacgagacc gagctggtcg
     3421 tggcgctcgc caagggcgga cggcgcatcg acgccaagcg cgccaacgac catgtgttcg
     3481 gctacgccgt cggcctcgac atgacgcgcc gcgacctgca gcagtggggc aaggatcacg
     3541 gccggccctg ggacttcggc aaggatttcg acgaggccgc accctgcagc gcgctcgtgt
     3601 cggcggcgaa gatcggccat ccggcgcgcg gcgcgatctg gctcacggtc aacggcaagc
     3661 agcgccagcg cgcagacctc ggcgacatga tctggtcggt gcccgagcag atcgcctacc
     3721 tctccgagta ctacaccctc gagcccggcg acctcatcta ctccggcacg cccgcgggcg
     3781 tcgggccggt gaagcccggc gacgagctgc acgccggcgt agacggcgtg ggtgagctga
     3841 aggtgaagat tctgcctgcc ttgtgaagct gtacacgtac ttccgcagct cggctgcgtt
     3901 tcgcgtccgc atcgcgctca atctgaaggg gctgccctac gaaccggtat tcgtgcacct
     3961 cgccaagggt gagcaccgca aaccggagta ttcgagcatc aatgcgcaag cgctcgttcc
     4021 cacgctcgaa ttcgatgacg gcacacgcct caaccagtcg ctcgccatca tcgagtatct
     4081 cgaggagaag cgtccgcacc ccgcgctcct tccggcagat tccctgggca aggcccgcgt
     4141 gcgcgctctc tcggacctga tcgcgtgcga gatccacccg ctcaacaacc tgcgcgtgct
     4201 gcagcacctg aagcgcgttc tcggacagag cgaagatcag gtcaacgcgt ggtaccgcca
     4261 ctggatcgct gacggtctgg cgaaattcga agccgagctg gccagcgaca agagaggaaa
     4321 gttctgctac ggcgactcgc cgaccatggc cgactgctgc ctggtgccgc agattttcaa
     4381 cgccaagcgc tacgactgcg atctcaatcc ctatccgagc accgtgcgcg cctttgagca
     4441 ctgcatgaag ctcgacgcct tcgaccgtgc gcagccggcg aagcaacccg acgcagagtg
     4501 aaccgatgaa gccgaacgaa tacgacgaca tcccgggtac cacggtgttc gacgcacagc
     4561 gctcgcggca gggctaccac ctgaacatgt tctgcatgtc gctcatgaag gccgagaacc
     4621 gcgcggcgtt caaggcgagc gagcgggcct atctcgaccg ctaccccatg accgaagaac
     4681 agaagcaggc ggtgctcgac cgcgactgga accgcatgat cagcctcggc ggcaacatct
     4741 acttcctggc gaagctcttc ttcaccgacg gcaagtcgtt tcaatacgta gccgccgtca
     4801 tgaccgggag cacccaggaa gagtacgcgc agatgatgct gaacggcggc cgcccgccgg
     4861 agggcaaccg ctacgtcgcg gagtgggaaa agcagccgga ccgcaagcgt ggctaggatc
     4921 gtcgccgggg tcgcgacttc gcacgtgcct gcgatcgccg cggcgctcga tctgggcaag
     4981 agcggcgagc cctactggaa gcgcgtgttc gacggctacc agcgctcgaa ggagtggctc
     5041 cgcaaggtgg acccggacgt catcatcctg gtctacaacg accacgcctc ggcgttttcg
     5101 ctggagatca ttcccacctt tgcgctcggc tgcgcggcgc aatttccgat cgccgacgag
     5161 ggctgggggc cgcgcccggt gccggcggtg aaagggcacc cggagctcgc cgcgcacatt
     5221 gcgcagtcgt tgatcctgga cgagttcgac ctcaccatcg tcaaccgcat ggacgtcgat
     5281 cacggcctca cggtgccgct ttccctgatg tacggccagc ccaaagagtg gccgtgcccg
     5341 gtgatcccgc tggccgtgaa cgtcgtgcaa taccctccgc cgacgggccg gcgctgctac
     5401 cacctggggc gcgccatccg caaggcggtg gagtccttcg atcagaacct gagagtcgtg
     5461 gtgttcggca ccggcggcat gtcgcaccag ctgcaggggc cgcgcgcggg gctgatcaac
     5521 aaggcgttcg acgcccggtt tcttgacgcg atcgcgcgcg atcccgccgc gctcacccgg
     5581 atctcgcacg tcgaatacct cagggaagcg ggctccgagg gcatcgagct ggtgatgtgg
     5641 ctcgtcatgc gcggcgctct cgacgacgag gtcgaggaag tgcaccgctt ctaccacgtg
     5701 ccggcgtcga acacggcgct cgggcacatc attctggaaa accgcaaatg aagatctgcg
     5761 tcgcaggaca gggcgccttc gggcagaagc acctcgaagg catcgccaac atcaagggcg
     5821 tcgaagtcgt gtctctcgcc ggcggcaatc ccgaggcgac ggcggaggtg gcgcgcaagt
     5881 tcaagatacc gcacgcgacc ggcgacctcg cgcagagcct caagcagccc ggcgtggagg
     5941 cggtgatcct cgcgacaccg acgcagatgc acgcgcgcca ggcgatccag tgcctgcgcg
     6001 cgggcaagca cgtgcaggtc gagatcccga tggccgacac cctggccgac gcgcgcgaga
     6061 tcgtgaaagt gcagaaggaa accgggctgg tcgcgatggc ggggcacacg cgccgcttca
     6121 acccgagcca ccagtgggtg cggaagaaga tcgtcgccgg agagctcagg ctcctgcacc
     6181 tcgtggtgca gaccttcttt tttcgccgca ccaacatgaa cgctgcaggc aagccgcgca
     6241 gctggaccga tcacctgctg tggcatcacg cctgccatac cgtcgatctg ttccagtacc
     6301 aggcgcgcga gccgatcgcc aaagcgcgcg cggtgcaggg gccgatgcat ccgacgctcg
     6361 gcatcgccat ggacatgagc atccagatga ccacaccctc gggcgtcgcg tgctcgctcg
     6421 cgctgtcgtt caacaacgac gggccgctcg gaaccttctt ccgctacatc tgcgacaacg
     6481 gcacctacat cgcgcgctac gacgatctgt tcgacggcaa ggacaacaag atcgacgtat
     6541 cgaaggtcga tgtctcgatg aacggcatcg agctgcagga ccgcgagttc ttcgccgcga
     6601 tccgcgagaa gcgcgagcct aatgcgagcg tcgcgcagtg cctgccggcg atggaagcgc
     6661 tcgaccgcct ggagaagagc ctgtcggctc cttgaacgct acctggcgag aaacagcgcc
     6721 ctgacgtcgt cggggatctg ctgacccttg agcggcgtgc ccgggccgtt gacatagcgg
     6781 ccccacacgc gcttttcgct gccctttgcg agcgtggtgc cgtcgctgcg caggacatca
     6841 tgcgccacca cgaacgaagc tccgccgaag cgcgcgatgc gcgagcgcac ctcgcagcgg
     6901 tcgccatgct gcgccggggc gagaaactgg cagtcggcgc cgaccagcgg catcgcgagg
     6961 tgctcgtcgc gcatgcgctt gggcccgtag ccgacggagc ccatgaaggc ccaggtggcc
     7021 gcatccatcc agcggaaata cgtcggatag aaaatgatcc cggcggggtc gcagtccccc
     7081 cagtgaacct cgcacaaaaa atgaccgacg atcgactcaa actcgctcat cgaatcgatt
     7141 ataagccgtt gacagagcag ggcttgatgg gcagaatccc acgcgccgcg ccgatttgat
     7201 gctcagcttg cgggcgcgtt acaaatgcag ctaaagaggc gcgcccagga gcccgttctc
     7261 tgcgcaagcc gagcgggctt tttgctttaa gcgatggaca aactcaacac actgctgcgc
     7321 gagaggatcc tgctgctcga cggggccatg ggcaccatga tccagcgcgc ccggctggcc
     7381 gaggaggact accgcggcgc gcgtttccgc gactggccgc gcgagctgcg cggcaacaac
     7441 gacctgctgt cgctgacgca gccccgcctg gtgcgcgaca tccacgcgca gtacctgcag
     7501 gccggcgccg acgtcatcga gaccaacacc ttcaactcca cctcgatcgc gctcgccgac
     7561 tacggcatgg aagcgctcgc ccgcgagttc aacagcgcgg ccgcgcgcct cgccaccgac
     7621 gccgcacgcg agtgggcgtc gcgcacgccg aagcagccgc gcttcgtcgc cggcgtcctc
     7681 ggcccgacca acaagaccgc gtcgatctcg cccgacgtca acgatccggg attccgcgcc
     7741 gtcagcttcg acgagctggt cacggcctac gccgaggcga tcgacggcct gctcgagggc
     7801 ggcgtcgatc tcctgctcgt cgagacgata ttcgacacgc tgaatgccaa agccgcgctc
     7861 ttcgccatcg agcaatgctt cgcgcaaagc ggaacacggc tgccggtgat catctcggga
     7921 acgatcaccg acgcctcggg acgtacgctc tccggccaga ccgcggaggc gttctacaac
     7981 tcggtgcgcc acgcgaggcc gcttgcggta gggcttaact gcgcgctcgg cgcaaaggag
     8041 ctcaggccct acgtggaaga gctcgccggg attgccgaaa gctacgtctc gtgccatccg
     8101 aatgcggggc tgccgaatgc gttcggcgaa tacgaagaca cgccggagtc gatggcggcg
     8161 caggcagccg aatgggcgcg cgccggctgg ctcaacctcg ccggcggctg ctgcggcacc
     8221 acgccggagc acattcgcgc catcggcgag gcgctgcgcc cgatcccgcc ccggaaaatc
     8281 cccgacaggc cgcgcgcctt gcgcctcgcg ggcctggagc cgctcaacgt cgacgaccgc
     8341 tcgctcttcg tcaacatcgg cgagcgcacc aacgtcaccg gctcgaaggc cttcgcgcgg
     8401 ctcgtgctcg ccggcgacta cgccggcgcc ctggcggtgg cgcgccagca ggtcgagaac
     8461 ggcgcccagg tgatcgacgt caacatggac gaggcgatgc tcgactcgga gaaggcgatg
     8521 agcaccttcc tgcgcctggt cgccgccgag ccggatatct cgcgcgtgcc ggtgatgatc
     8581 gacagctcga aatggagcgt gatcgaggct gggctgaagt gcgtgcaggg caagccgatc
     8641 gtcaactcga tctcgatgaa agagggcgaa gagacattct tgcaccacgc cgcgctttgc
     8701 cggcgctacg gcgccgcggt cgtggtgatg gccttcgacg agaaaggaca ggcggacacc
     8761 ctcgagcggc gcactgcggt ctcccgacgc gcctatgacc tgctggtcaa caaggcaggg
     8821 ttcctgcccg aagacatcat cttcgacccg aacatcttcg ccgttgccac cggcctcgag
     8881 gaacacaatc ggtacgcgat cgacttcatc gaggcggtgc gctggatccg caccaacttg
     8941 ccgcagacga gggtatcggg gggcgtctcc aacctgtcat tctcttttcg cggcaacgac
     9001 ggggtacgcg aggcgatgca caccgcgttc ctgtaccacg cgatccgcgc cggcctctcc
     9061 atgggcatcg tcaacgccgg gcagcttggc gtctacgagc agatcgagcc cgagctgcgg
     9121 ctgcgggtcg aagacgtgct gttcgatcgg cgccctgacg ccaccgagcg tctggtggag
     9181 ttcgccgcga cggtgaaaac cggtgcgcgc gaaaccgcca aggatgaagc ctggcgcacc
     9241 ggcagcgtcg agcagcgcct cgctcatgcg ctggtcaacg ggatctcgag ccacatcgtc
     9301 gcggacaccg aggaagcgcg gcgcaagtcc gcccggccga tcgaggtgat cgaggggccg
     9361 ctgatggacg gcatgaacgt ggtcggcgat cttttcggcg ccggcaagat gttcctgccg
     9421 caggtggtga aatcggcgcg cgtgatgaag caggcggtcg cgcacctcgt gccctacatc
     9481 gaggcggaaa aagcgaagag cggcgaagcc ggccgcgcga aggggcgcct ggtgctggcc
     9541 accgtgaagg gcgacgtgca cgacatcggc aagaacatcg tagcggtcgt gctccagtgc
     9601 aacaactacg aggtggtgaa cctcggcgtg atggtccctg ccgagaccat cctcgagacc
     9661 gcgcggcgcg agaaagccga cgtcatcggc ctctccggac tcattacccc gtcgctcgag
     9721 gagatggctc acgtcgcgcg cgagatggag cgcacgcagt tcaagctgcc gctcctgatc
     9781 ggcggcgcca cgacctcgcg cgcccacacc gcggtcaaga ttgcgcccaa ctattccggc
     9841 cccgtggtgt acgtcccgga cgcctcccgc agcgtgcccg tggtgcaaaa cctcctgtcg
     9901 cagaagccgc aggcctatct cgccgaggtg cgggccgact acgagcgcat ccgcatccag
     9961 caccgcgaca agaagggccc cggcccgctc cacccgctcg cgcgcgcgcg cgagctcggt
    10021 ttcaaggtgg attggcgaaa gtacctgccg cccgctccgg cgaaggcagg cctgactgcc
    10081 ctgcgccgct atcccctggc ggagctggtc gaccacatcg actggggacc tttcttccag
    10141 gcctgggagc tctcgggccc ctacccaaag atcctggagg acccggtggt cggcgaggcg
    10201 gcgcgcaagc tcttcgccga ggcccaggag acgctggcgc gcatcgtgcg cgagaacctg
    10261 gtcgtggcga acggcgtgtt cgcgctctac ccggcggcgc gggtcgatca cgacgacatc
    10321 gagatctacg ccgacgagga gcgccgcaag ccgctcatga cctggcacaa cctgcgccag
    10381 cagaatcaca agcccgaagg acgcgccaac ctgtgtcttg ccgatttcgt ggcgccgaag
    10441 gcgagcggcg tgcgcgactg ggtcggcgcc ttcgcggtca gcgccggcgg catcgaggag
    10501 cgcgtcgccg agctcgagaa gcgcggcgac gactacaacg ccatcatgct gaaggttctg
    10561 gccgatcgct tggccgaagc ctttgccgaa cggctgcacg agcgcgtgcg ccgggaattc
    10621 tggggttatg ccgccgagga gcgcctgagc ggcgccgagc tgatcgccga gcgctatcgc
    10681 ggcatccggc cggcgccggg ctatcccgcg tgcccggatc acaccgcgaa gggaccgctg
    10741 ttccgcctgc tcgacgccga aaagaacgcc gacatgaagc tcaccgagtc cttcgccatg
    10801 ctgcccgcgt ctgcggtttc cggcttctac ctttcgcacc cggaggcgag ctatttcgcg
    10861 gtcgggaaga tcggccgcga ccaggtcgag gattacgcca gacgctcccg catgagcatg
    10921 gccgaggccg agaagtggct cgcgccctat ctggcgtacg agctcgaaac cgtataatcg
    10981 ggctcatggg aaaaggcgac aagcgcacgc ggcgcggcaa gatctacaag ggcagctacg
    11041 gcaagaagcg cccgcatcgg caaaagccga agccgaaaaa aacctagcta gctttcttcg
    11101 cggcgcccga ccgggagtcc gagcgccttg agcttccggt agaggtgcgt gcgctcgagg
    11161 cccgagcgct cggcgacgcg cgacatgctg ccgtgctcgc gcgcgaggag atgctcgaag
    11221 tacacgcgct cgaaggcttc gcgcgcttcg cggtagggac ggtcgaacgc cacctcgggc
    11281 agcgtaccgg gcacggcgcg cgccgcgagc acgcgctcga catcctcgag ctggatctct
    11341 tcgtccagcg cgctcagcgc caggtccttc accacgctct ccagctccgc gaggtttccc
    11401 ggccaggtat ggtgccgcag ggcgttcagc gcggcgatgg cgaagcgccg ggccgggcag
    11461 accctggcct cgaccagctg cgcaagcatc agcgaggcga gatcgggaac gtcctcggcg
    11521 tagtcgcgca ctgcgggcag cttgagcgtc agctcgccca ggcgcgcgcc gagctcggga
    11581 tcgaaatcgc acttctccga cagggtgcgc acgtcgagcg gcgaaaacga caccaggcgc
    11641 acgcgatgct tctccgcgcg cggcagcagg aactcgaggt tgcgctgctc cgcatgaccg
    11701 agccgcgaca ggtccgggat gaacaggatc ccgccggcgg ccttgccgag cagctccgag
    11761 ggcgactgcg acagcatctc gccgccggcg aggaaaggcg caccgggcgc ggcaagcagc
    11821 cgcgcgaaaa gctcggcgcg catgccgcgc tccccgcgca gcaggagcgg cgcgcccgag
    11881 ccggcgagct gcgccagccg cttcttcgct tccgccagcg ccggcgcgcg gccgaacgcc
    11941 gccatcgaaa gctcgggcgc cgccgcggtc tccgggttgc gcagcgcccg cttcaccgtc
    12001 gcgagcaggc gctgcatcgc gatcggcttt tccaggaaat cgagcgcgcc gatgcgcgtc
    12061 gcttccaccg cggtctcgat cgtggcatgg cccgacatca tcacgaccgg catggtgagc
    12121 tgaccgctcg ccgcccactc cttgagcagc gtgatgccat cggcgtcggg catccagatg
    12181 tcgagcagca cgaggtcggg ccggccgcgc tcgcgcaagc gccgcgcgtc gcccgcgctc
    12241 tcggcgagca ggacctggtg gccctcgtcg gcgaggatct cggagagcag ctcgcggatg
    12301 ccgatctcgt cgtcgaccac caggatctgg gccatggcgc taggcagcct tggcgagcgg
    12361 cagcagcacg ctgaccgagg cgccgctctc cggacggttc tcgatcgtca cggcaccgtg
    12421 gtgctcgtcg acgatcttct tcacgatcgc gaggcccagg cccgtgccgc gcggcttggt
    12481 ggtgacataa ggctcgaaga tccgcgccat cagctcctcc ggaaagccgg cgccgttgtc
    12541 cgacacggcg agccggatgc ggtcgccggc cagctcggtg cgcacctcga tcgccgggct
    12601 cgcgccgcgc cccgcctgct gctccagcgc atcttgcgcg ttctgcacca agttgtggat
    12661 cacctggcgg atctgcgcgc cgtcggccca caccgggggc agcggctcgg caaggtgctt
    12721 ggcgatcggc acggtcgaat tttcgtacag ggcgagcacc tggcggacca gagcgttcag
    12781 atcgaggggc gcgggctccg gcgccggcag ccgcgcgtat tcccggaaat cgtccaccat
    12841 cgccttcagc gccgcaacct ggttgacgat ggtctcagtg gcgtgtttga gcgtgtccgc
    12901 atcctcgcgc ggcaaccgcg cgccgagctt catctcgagg cgctcggccg agagttggat
    12961 cggggtgagc gggttcttga tttcgtgcgc caggcggcga gccacctctg cccacgcggt
    13021 ggcgcgctgc gcctggacga gttgcgtgat gtcgtcgaac acgaccaccg cgccgccacc
    13081 ggtcgcgagc ggcagccgcg cgccgcgggc gtggagcgtc ttgccggtcc cgcggaggtg
    13141 caactcgagc tgccagtcgg cttcgccttt ctcggcgaaa tgggcgcgca tctgggcggc
    13201 gaaagcgtcg agctcgggcc cgaggatcgc gccggcgccg tgattggaaa tcgaaagctc
    13261 gagcgcatgg tcgaacacca gcacgccggc cgacaggttg gcgaggatgt tctcgagccg
    13321 cgccttggcc ccctcgagcg caagctggtt cgactccacc ccgcgccgcg cctcctcgag
    13381 ctggcgcgtc atcgaattga acgactcggt gagcacgccg agctcgtcgc ggctggtgac
    13441 gggcgcgcgg cgcgagaaat cgccccgcgc caccgcctgc gtcgcctgcg cgaggttggc
    13501 gagcggctcc gccagcaggc cggattgcgt ggcagccacc gcgaccgcga cgaacagcgc
    13561 catcagcagc gcgaaagtca gcgtgacgat gtagatgcgc ttcaatccct cgcgcgagat
    13621 cgcgagctcg cggtagtcgc gatacgccgc ctcgaccgcc tcggcgctct gggcgaacga
    13681 cgccggcacg ctttggcgca attgcaggaa ccgcatttcc ccgggaaagt ccaccggcac
    13741 gatgacacgc agcgacagcg gacgccccac ggtcgcgtcc acctcggtat ggctgcgctt
    13801 ggcccgcgcc tgccgcagcg cctgtcccgc gggaagctcg ggcacgagct tggtgacgtc
    13861 gtgcgccgcg ctcgcgacga gccgtccgct tcctgtcact atgactgcct cctccacgcc
    13921 gagctggccg cgcaggcgct cgagctgggt cgcgagctcc gacggggagc gctcggcgag
    13981 ctcgagcgcc atcgcgttcg ccttgccctg caaatcgatc gtcatctgct cgatagcctg
    14041 ctggccgagc ttgatgccgc cttccagagc cgcgtcgacc ttgacgtcga accacgattc
    14101 gatcgagcgc gtgagaaact gcaccgacac ggcgtacacg atgaggccgg gcaccaccgc
    14161 gagggcggcg aagcgcgcga gcagcctgag ggtaaggcgg gcgccgaaca cgcgcgcgcg
    14221 atggcgccgc gcgatcatta cgagctggat gccgacgagc gcggcgagga gcgcggcgag
    14281 cgccgcgttg aggccgagca gcaggggata gtggcgcgcg aagatcgtgg tgtccgcgct
    14341 cgccgtcgct agcaggaaaa gtcccaccgc cgccaccgcg ccgccgacga ggataagcca
    14401 tttcatcggg gcgcccgcaa gatgaaccgc ttccaggccg actcgagctg caggtcgcgg
    14461 ccggtcagcg cagacagctg aaacggcttc ggcagcagcg tcgtgtcgag gcgcatgcgc
    14521 accgcggcgt cgtagtcggc gtcggccagg cgcacggcgc gctccaccac cagccagttg
    14581 cgcacgcgct tcagcacgtt gagcgcctcc tcgagcgtgg gaaagctctg ttgcagcagc
    14641 ccggttgaca gccggtactg ccgcgacagc gcatgatagg acagccgcag ctgcagctgc
    14701 tgcgacgccg cggtatcgtc gaaccagtac cagcgccgcc gcgtcagctc gaagtcgacg
    14761 cggaaataga gcggcacgcc gttccccacc acgtcggcga gacgcggcgt aagctcgaac
    14821 gcgaaatcgg cgtcgagcgc gagcccttct tcgacgctgc gcagcgccac gtcgcgcacc
    14881 tcgatgtcct cggcgcgcgg cgccgccgcg atgatcgcca tcgcgagaaa cccgccgatg
    14941 atgatgctga ctcgggcgat gcggcgcatc gcgaagccag tcagtcttgc ttgtggatca
    15001 gcgcgtagta gaacccgtcg cgctccgcgt cgggcagccc ctgcgccgca gcgccgtcgg
    15061 gaagcggcac gcgcacagcg cgcgccgccc gagccaggaa gccctcgatc acggcgtcgt
    15121 tttcctgcgg aaacaccgag caggtggcat acagcaattt accacccggg caaagcacct
    15181 gccaaagcgc atcgaggatc tctgcctggc gcgcggcaaa tgcggcgagg tcgtggctgc
    15241 gacgcaacca tttgatgtcg ggatgccgcc gcgcgatccc ggaggcggag cacggcacgt
    15301 cggcgaggat gcgatcgaac ggcactcggt cccaccagtc gcccggccgg gtgcaatccg
    15361 cgttgacgac gcgcgcgccg agcccgaggc gcgccaggtt gcgctcgagc cgctcgcggc
    15421 gcgccgcgtc cagttcgagc gccgtcaact ccacgtcggc cgtctcgaga acgtgaccgg
    15481 ccttgcctcc cggcgcggcg caggcatcga gcacacgctg gcctgcggcc agctcgagac
    15541 atctcgccgc gcgctgcgcg cccgaatcct gcaccgagac ttccccggcg gcaaagccgg
    15601 gcaggcgttc gaccggcacc ggccgctcga gcagcaacgc ttcgtgcccg acttcgcgtg
    15661 ccgcgatgcc ttcggcggcg agtttcgccc gatagtccgc gacgctcgag cggcgccggt
    15721 tcacccgcag gcacatcggc ggatgcgagt tcccggccgc gagggtctcg gtccatcctc
    15781 cgggatagca ctgcttgagc aggtcgatcc accagcgcgg atgctggtag cgcgcttcgg
    15841 cgtcggcccg gatgcgtgcc tcgagcgatg tgcgctcgcg caggtagccg cgcagcagcg
    15901 cgttcacgaa ccccttggcg ctccagcgct cgagcatcgc gcaggcgcgc accgcctgat
    15961 cgacgacggt gtaatcggcg tagcgccccg actcgagcgc gtaaagcgag caccacagca
    16021 gagcttccgc gaccggatcg ccgcgctcgc ggcgcgacag ctgcctgacg attgcctgca
    16081 cgcgtccgta gcgccgcagc gtgccgtgcg tgagatcgat cagcgcggcg cgcggcgtcg
    16141 acgccccttc gtcggcgaag cgctcgaatt cggtcgcaag gctcgcgccc gcggcgacgc
    16201 gcgcgacgat ggtcgccgca tcgcgcagcg cggtcgcgag cgcgatcact cgaagcgcgt
    16261 accctgcgcg agcctgtagc cgcgcacgaa ctccgcggcg ctcagccggc gcccgccggc
    16321 acgctgcagc tcggaaactg ccaacgcccg ctcgccgcag gcgattacca ggctttcctg
    16381 cgagtcaaga acggtcccgg gcgcgccgcg ggcgtcgacc acgctggcgc gccagagctt
    16441 gaccggctcg ccgtcgagca aggcgaatgc gcccggcgcc ggccggaagg cgcgcacagc
    16501 acgctcgagg tcgcacgccg gccgcgacca gtccaagtgc gtgtcgcgct tgccgatctt
    16561 cgccgcgtag gacacgccgc tctccgcctg cggcgtcggt cgcgcccggc ccgcctcgat
    16621 ctcgtccagc gccgcgacga tcgcttcggc tccctggacc gcgagcttgt cgtgcagcga
    16681 gcccgcatcc tcgtccggcg cgatcgcaat gcgcttctgc atcaagatgg gcccggtatc
    16741 caggcctgcg tccatcagca tgatcgagac tcccgtctcg gtgtcacccg cgagcagcgc
    16801 gcgctgaatc ggcgccgcgc cgcgccagcg cggcaacagc gaggcatgaa tgttcaaagc
    16861 gccgtggcgg ccggcgtcga gcagctcgcg cggcagcagc aggccatagg ccgcgacgac
    16921 aagcgcatcg gggcgcatgg cgcgcaccgc ctccacctcg ggagcgcctc tcaaccgggg
    16981 cggctggaga acagcgatgc gccgcgcggc ggcgaagcgc ttcaccgggc tcgccagcgg
    17041 ttgcaggccg cggccggcag gacggtcggg ctgcgtaagg acgagggcga tgtcgtggcc
    17101 tgcggccgcg aggcgcgcca gagcccgctc agcgaactcg ggcgttccgg cgaagactag
    17161 gcgcaggaaa gcgggacgtt caggccgact tgcgcagctg cttggcgagc ttcgcgcgga
    17221 tgcgctgctg cttgagctgc gacaggtgct cgacgaaaac cttgcccttc agatggtcca
    17281 tctcgtgctg gatgcacacc gcaagcagac cctgcgcctc gagcgtgaac ggattgccgt
    17341 tctggtcgta cgcccgcacc ttcacgcgct cggcgcgctc gaccagctcg tagatgccgg
    17401 gcaccgagag gcagccttcc tcgatgtccg aaactccggt cgcttcaagg atctccgggt
    17461 tgacgagcac cacgagcgag tcgcggcgct cggagacatc gaccacgatc acctgcttcg
    17521 gcacatcgac ctggatcgca gcgaggccga taccgggggc ggcgtacatg gtttcagcca
    17581 tctcggcgac cagcttcttc aggctgtcgt cgaagacggt gacgggggcg gctaccttat
    17641 ggagccgggc atcggggtac cgaaggatgg tcaaacgggc catttcactt gcgaattgaa
    17701 tggattatgt ggacaatctc aggcagcgta taaacaatcc aacctttagt gctacattgg
    17761 ggtggaaaaa tcgagtttcc agccggtccg ggaaccgcga aagggacggg tcgccatggt
    17821 ccagcaagcc tgtaagtcta tcatagcgct cgctttcttg gtgcctgggg cgcttttcgc
    17881 ccaggcgccg agcacaccct cagcggcccc tgcaacgtct gctccatcgc ccgcccgctc
    17941 gtctgctcct ctggcaatca aacccgatgc tcccgaccga tataccgtgg tcccgggcga
    18001 cactctctgg ggcatttcgc agcgctacac ggattcgccc tggcgctggc cagagttgtg
    18061 gaacatgaac cgcgagcaga tccgcaaccc ccatctgatc taccccgggt acgtcatcgt
    18121 gctggaccgc gagcgcgcgc aactcaccat cgctgctccg ggcaccgagc gtgcaccggg
    18181 gacgacgccg gcgcccgggg tgccgccgtc caccaccacc gaaccaacta cgccgtcgac
    18241 cacgacgacc acgacggccg ggacggcggg gacggcgggg acggcacccg gcacggtcag
    18301 gctcggaccg cgcgtacgcg cggagtcgct cgccaagcag gagatcccga gcatcccgcc
    18361 ttccgtgatc gagcctttcc tttcgcgccc gctcgtagtc gagcccgacg ggctcgaaaa
    18421 agccccgacg atcatcggga cccaggccga tcgcgtggtc cttgccgcgg gcaacgcggc
    18481 ttatgtgcgc ggcatgggca cctccaagga cgagaactgg cacatctatc gaagaggcgt
    18541 cgcgctggtc gaccccgata ccaacaagac cctagggtat gaagcagtct atctcggcac
    18601 ggcgcagctc acgcgaccgg gcgagccagc gacggtcgtg ctgaccagcg cggtgcagga
    18661 ggtcgagctc ggcgacaaat tggtggctgc aggccgtccg cagccggtga actacgcgcc
    18721 ccgcgcgccg gccgcgcaaa tcaagggacg cgtcatcgcc atctacggcg gcgtcgccca
    18781 ggtcggcgaa gccgggccgc agcagatcat cagcatcaac cgtggcaagg ccgacggcat
    18841 cgaagtcggc cacgtgctcg cgctctactc gcttggcggc acggtgcgcg atgcgaccaa
    18901 gccgcgcagc gcggccaatg cgcgcattcc gctgcccgac gaacgcgccg ggctcgcatt
    18961 tgtgttccgc gtcttcgacc ggatatccta cgcgctgatc atgcagatca cgaggcccgt
    19021 cagcccggcc gatgtggtgc agacgccgta acagaagcaa gaagtgaatt cggacccggg
    19081 gctcgcgagc tggctgcagc tcgcccttac cccgggcctg ggtgccgcaa ccttgcagcg
    19141 catgctgcgg caattcggcc tgccgcaggc ggcgctcgcc cgaaagcgcg cggagctcgg
    19201 agccttcacc acgccggcgg ttctcgcagc gctcgactcc gaagcggtcg cccacgccgt
    19261 cgaacaggcg ctggcatggg cgtcacagcc gggccacgcg gtcatcaccc tggccgacga
    19321 cacctacccg cggctgcttc tcgaaatttc cgatccgccg gcgctgctct atgcgcgcgg
    19381 ccgcaccgag ctgctttcgc gcactgcgct cgccatcgtc ggcagccgca atgccacgca
    19441 gcagggcgaa gcgaacgcgg aggcgctcgc caaagctctc agcaacgccg ggttgaccat
    19501 cgtttccggg cttgcgctcg gcatcgatgc ggcggcgcat cgcggcgggc tcgccggcgc
    19561 cgggtcgact atcgccgtgc tcggcaccgg aatcgatgtg gtctatccga gccgcaacgc
    19621 cgcgctcgcc gccgagatcg ccgagcgcgg cgtgctgatc tcggaatttc cgctcggcgc
    19681 ggcgccggcg gcgcagaatt ttccgcgccg caaccggctg atcagcgggc tcgcccaggg
    19741 gtgcctggtg gttgaagccg cgctcgcctc cggctcgttg atcacggcgc gcgctgccgc
    19801 cgagcagggt cgcgaagtgt tcgccattcc gggctcgatc cattcgccgc tcgccaaggg
    19861 ctgccacgcc ctcatcaagt cgggcgcgaa gctggtcgag tcggcggaag acgtgctcgc
    19921 cgagctctcc ggattccggc cgagcgggta cgcatcgact acggcttcaa cggcgaaagc
    19981 gccgcccgcg gccgcggatg ccggcctgct cgcctacatg gggcacgacc ccgtcgacgt
    20041 cgactcgctg tgtgcgcgcg ccgggatgtc cgcggagcag gtgtcgtccg agctgctgcg
    20101 gctggagctc gacgggcgcg tggcggcgct gccgggcggg ctataccaac ggctggagaa
    20161 gggtcaacgc cggtaaacgc aatccccatc gtcgcgttat aagaagggag cgccatgtac
    20221 gacatcctcg tattcgtgtt cgagaactgc cagcaggccg acgtcgccta cgatcgcgag
    20281 cgcgtcgcga agaaactttc ggcggccggc ttcgaggatt cggacatcag cgaagcgctg
    20341 cactggctgg ccggcatggc gcgcgcgccg cagggcgtgc gcgagccgct gcccgacgcg
    20401 cgctcaagct tccgcgcctt cgcgccgcgc gagctgatga agctcgacgc gcgatgccgc
    20461 ggtttcctcc tcaccctgga gcaatcgggg attctcagcg ccgaaacgcg cgagctggtg
    20521 atcgagcgcg cgcttgcggc ttccggcgaa gccctcagcc tcgagcagct caagctcgtg
    20581 gtcctgatgg tgctctggaa ccagcagacg gcgaccagcc ggctggtcgc ggaggacctt
    20641 tttaccgcag cgcacgctcg cctcgcgagc taggtctttt cacgacgggt tgaagcgccc
    20701 gcgcgtttgc acttatcatc tgcggccttt cgcgccgcat ggccaaaaaa ctcatcatcg
    20761 ctgagaaacc gtcggtcgcc gccgacatcg cgcgggcgct cggcggcttc aagcgcagcg
    20821 gcgaatactt cgaaagcgag cgctacgtgc tgtcgtcggc cgtcggccac ctgctcacgc
    20881 tcgcggtgcc cgaagagcac gacgtcaagc gcggcaaatg gtctttcagc cgcttgccgg
    20941 tgattccgcc gcatttcgcg cttgccccga tcgaaaaatc cgagacccgg ctcaatctgc
    21001 tgctgcgcct gatgaagcgc aaggacgtga ccgggctggt gaacgcctgc gacgcgggcc
    21061 gtgaagggga gctcatcttc cgctacatcg tgcagcacgc gcgcaacaag aagccgatcg
    21121 agcggctgtg gctgcagtcg atgacccaac agtcgatccg cgaggggttc gagaagctgc
    21181 gcgcggacaa ggagctgctg ccgctcgccg atgcggcgaa gagccgctcc gaggccgact
    21241 ggctggtcgg catcaacggc acgcgcgcca tgactgcctt caactccaag gaaggcggct
    21301 tctatctcac cacagtgggc cgggtgcaga ccccgaccct ggccatcctg gtcgagcgcg
    21361 aggagcgcat ccgcgccttt acggcgcgtg actactggga agttcaggct cgattcggcg
    21421 caaaggcggg cgagtacccc gcgcgctggt tcgatccgaa gttcaagaag gacgacgata
    21481 ccgagcgcaa gcccgagcgc ctgtggagcg aggccgaggc caaagcgatc gtggcggcgt
    21541 gccgcgggaa gaccggcatc gccacggagg aaagcaagcc ctcgacgcag ctttcgccgc
    21601 tgctctatga cctgacgagc ctgcagcgcg aggccaacag cggcttcggc ttccccgcgc
    21661 gcatgacact gtcgcttgcc caggcgcttt acgagcgcca caaggtcctt acctaccccc
    21721 gaaccgacgc gcgagcgctg ccggaggatt acctcgctac ggtcaagaag acgctggacg
    21781 agcttggcga cacgcgcgag ttcgcgccgt tcgccaagca gatcctgaag cagggttggg
    21841 tcaagccgaa caagcggatc ttcgacaaca gcaagatttc cgatcacttc gccatcattc
    21901 cgaccctgca gacgcccaag cacctgaatg aggcagagca gaagctctac gacatggtgg
    21961 tgcggcgctt ccttgcggtg ttctaccccg cggccgagta cctgcagacc acccgcatca
    22021 cgcgcgtggt cgagcatcag ttcaagacgg agggcaaggt gctggagacg cccggctggc
    22081 tcgcggtcta cggccgcgct ggcggcgagg aaaacgagaa tctgcccgcg atcgcgcagg
    22141 aggaaaaggt cccggtgcgc gagattgccg cgcacgccaa ccagaccaag ccgccgccgc
    22201 gctacacgga ggcgacgctg ctctcggcga tggagggcgc cggcaagctg gtcgaagacg
    22261 acgagttgcg cgaggcgatg gcggccaagg gtctgggcac gccggcgacg cgcgccgcga
    22321 ccatcgaggg cctgatccgc gaggattatg tgcaccgcga gggccgcgag cttgtcccca
    22381 cgccgaaggc tttctcgctg atgttcgcgc tgcacatgct gcacatcgtc gagctcggct
    22441 cgcccgagct caccggcgaa tgggagcaca agctgaagct gatcgaggcc ggcaagctga
    22501 cgcgcgcaga gttcatggac cagatcagcg cgctggtgaa gaccgtggtg aacaccatca
    22561 agcacggcga gatccccgac gtggtgtacg ccacggtgcc ggcgccatgc ccgaaatgcg
    22621 gcggcgcagt gcaggaaacc taccgcaagg tcgagtgcca gaaatgcgac ttctcgctct
    22681 ggcgggtgat ttcgggccgc gagtggacgc ccgaggagct cgccgagctc ttcaagaagc
    22741 gcttcgtcgg cccgctcacc ggctttcgca gcaagcaggg gcggccgttc agcgccggga
    22801 tccgcctcac cgacgagctg cgcctcgagt tcgatttcgg ccagggcgcg ggcgatggcg
    22861 acgaagcggt cgacttcagc gcccaggagc cgctcggcgc ctgccctaag tgcaagtcac
    22921 gcgtcttcga gcacggcgtg gcctacgtct gcgagaaggc cgtcgggccg caacgctcgt
    22981 gcgacttccg ttccggccgc ctgatcctgc agcagccgat cgagcgcgag cagatgaaaa
    23041 agctgctcga aaccggccgc accgatctct tgaccggctt cgtgtccaag aagaaccgca
    23101 agttcaaggc gttcttggtg aagcagcccg acggcaagat cggcttcgag ttccagccgc
    23161 gagcgccgaa agaaggaaag gaaaagccgg tggcgcccga gggcaaagct acacgtcgag
    23221 gttccgaacg ccgagcgcgt tgagctcgat gaacgcgcgc cgcggctcga cctcgtcgcc
    23281 catgagtttc gtgaagatct cgtcggcgac gatgctgtcc tcgatctgca ccttgagcag
    23341 ccgccgcgtt gccgggtcca tggtcgtttc ccacagctgc ggcgggttca tctcgcccag
    23401 gcccttgtag cgctgcaggg tcatcgcctt ctcgacctcg cccagcagcc acttcatcgc
    23461 ttctgcgaag tcggccaccg gctgacgctt gtcaccgcgc tgcaccgctg cgccggtgcc
    23521 gatcaggccg cgcagcattt ccgcggtctg gcggatctgc gcgtagtcgc ccgagtgcac
    23581 gaactccgta tcgatggtgc tcatgcgcac gttgccgtgg cgcgtgcgct cgacgcgcac
    23641 ctggtagcgc tcggtcttct ggtcgaagtg cggcgcgact gcgaagccct cgccgccgat
    23701 cgccgcttcg agcgcctcgg cggacgcttc cgccgtgtcg cgccgcgtca gatcgacctg
    23761 cacgccgcgc agcatggcct gcaatacttc gcggtcgacg aggcgcgaca cgcgctccat
    23821 cacggcctcg gcgagcaggt aggacttggc gagctccgcc agggtatcgc cgctgatcgg
    23881 ctcgcgcccc gcgccgggat gcagcgccgc gtcgaccagc gcctgcttca gcatgaaggc
    23941 gttcagctcg tgctcgtcct tgaggtagcg ctcttccttg ccgagcttcg ccttgtagag
    24001 cggcggctgc gcgatgtaga tgtgcccacg ctcgaccagc tcgcgcatct ggcggtagaa
    24061 gaaggtgagc agcagggtgc ggatgtgcga gccgtcgacg tcggcgtcgg tcatcacgat
    24121 gatgcggtgg tagcgcagct tgtccggatt atattcctcg gtgccgatgc cgcagcccag
    24181 cgccgtaatc agcgtcgcaa tctcggccga ggacaaaagc ttctcgaagc gcgcgcgctc
    24241 gacgttcagg atcttgccct tgagcggcag gatcgcctgg aacttgcggt cgcgcccctg
    24301 cttggccgag ccgccggccg agtcgccctc gaccaggtag agctcggaaa gcgccgggtc
    24361 gcgctcctgg cagtcggcaa gcttgccggg caggcccatg ccgtcgagca cgcccttgcg
    24421 gcgcgtcatc tcg
//
LOCUS       DATBSZ010000245         3399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2437650, whole genome shotgun sequence.
ACCESSION   DATBSZ010000245 DATBSZ010000000
VERSION     DATBSZ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2437650"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VIG70_15600"
     CDS             complement(<1..323)
                     /locus_tag="VIG70_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8252023.1"
                     /translation="MSPSKETRAEILVIDDDAIMRDLIVDWLEAAGYAVHKATDCNSA
                     CAALERAPALVVSDMWMPGPCGAEAIRWMRQKHPGLQLIAISGHFGSGQGCTEQDAAK
                     AGAART"
     gene            436..1185
                     /locus_tag="VIG70_15605"
     CDS             436..1185
                     /locus_tag="VIG70_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8252024.1"
                     /translation="MSLQTQGPIQAAVPHVLAVDDDPTIRELIADYLGQNELRVTAVA
                     DGRAMREVIAEQVVDLIVLDLRLKSEDGMALARGLRDESAIPIIMLTGRVEEADRVMG
                     LELGADDYLTKPFSPRELLARIRTILRRRRAEVRQGRPEGIRAYRFDGWELNLNTRRL
                     AATDGRNVPLSNGEFSLLVVLLGSPQRILTRDQLLDLSRLHNDEVYNRSVDVQIMRLR
                     RKIEKDPAQPRYIKTERGAGYVFGVPVETVY"
     gene            1251..1556
                     /locus_tag="VIG70_15610"
     CDS             1251..1556
                     /locus_tag="VIG70_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004529398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8252025.1"
                     /translation="MLGLPHAPVAFGPFMCVKVDNEVTLDFCDDDGSIVSQHYAFLVG
                     ENEFDQIFGRIKSRKLEYWGDPARAKPGETYTWNGGRGVYFLDPSGHFLEVHTRPYF"
     gene            1689..>3399
                     /locus_tag="VIG70_15615"
     CDS             1689..>3399
                     /locus_tag="VIG70_15615"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY8252026.1"
                     /translation="MIARIASTFCCVSLLAAFAPAFATSDHHAASIAGLKDRVSVVRD
                     RFGVPHISARNDHDVYFMQGYVHAGDRFFQMDASRRQASGTLAELLGPGALAGDVQLR
                     TLGLRRAAARSLAAYPAEVQAILQAYADGVNAWLAQGALPPEYAALELTRVPAWTTLD
                     TLAVGKLLAFGLSFDTIDLDNTRALLTYQGFGAALGFDGTKLFFDDLFRSAPFDPTIS
                     IPEGTVSGPVRERHGRGEHADGDSVDKARSFLERPSPFFAPHGRGEGGSNWWVVSGAK
                     TESRNPMLASDPHLSLSSPSVFHEVHLAVRRGHKQEPLDAYGVGFPGVPGVIHGHNQR
                     IMWGSTVNPMDVTDYFQEQIVLQGGIPVATMFRGAPEPITIIPETYRANIVGDGIQDS
                     VVPITAGVPPATFVVPRRNNGPLVTAPAADASGRVTAISVQYTGFSATREIETFLSFG
                     RAGSVAEFRQALQFFDVGSQNWAYADVDGNIAYFTSAELPLREDLQAGTVEGLPPYFV
                     RDGTGALRNEWIRKSDPPADQALAYEILPFAEMPQLVNPARGYIVNGNNDPIGVTLDN
                     NPLN"
BASE COUNT          555 a         1207 c         1103 g          534 t
ORIGIN      
        1 gtgcgggcgg cgccggcctt cgccgcgtcc tgctcggtgc agccctgccc ggagccgaaa
       61 tggcccgaga tcgcgatcag ctgcaagccc gggtgcttct ggcgcatcca gcggatcgcc
      121 tcggcgccgc aggggccggg catccacatg tcgctcacga ccagtgccgg cgcgcgctcc
      181 agcgcggcgc aggcggagtt gcagtcggtg gccttgtgga cagcgtagcc ggcggcctcg
      241 agccagtcga cgatcaggtc gcgcatgatc gcgtcgtcgt cgatcaccag gatttcggct
      301 cgggtttcct tgctggggct caagcgccca ttccacagga tggcaattgc gtgtaattta
      361 catccaccgc cgccccaagt tacagctgta acggcgtccg gcggctcctg actagacttt
      421 gcatccgggc caaccatgag cttgcagacc cagggtccca tccaggccgc agtgcctcac
      481 gtgctggcgg tggacgacga tccgaccatt cgcgagctga tcgccgacta cctcggccag
      541 aacgagctgc gggtcacggc ggtcgccgat ggccgcgcca tgcgcgaggt gatcgccgag
      601 caggtggtcg acctgatcgt gctcgatctg cggctcaagt ccgaggacgg catggcgctc
      661 gcgcgcgggc tgcgcgacga atccgccatt ccgatcatca tgctcaccgg gcgggtcgag
      721 gaagccgacc gcgtcatggg gctcgagctc ggcgccgacg actacctcac caagccgttc
      781 agcccgcgtg agctgctcgc gcgcatccgc accatcctgc gccgccgccg cgccgaggtg
      841 cgccagggcc gccccgaggg aatccgcgcc taccgcttcg atggctggga gctcaacctg
      901 aacacgcgcc gcctcgccgc gaccgatggg cgcaacgtgc cgctgtcgaa cggcgagttc
      961 agcctgctgg tggtgctgct cggctcgccg cagcgcatcc tcacgcgcga ccagctcctc
     1021 gacctctcgc gcctgcacaa cgacgaggtg tacaaccgct cggtcgacgt gcagatcatg
     1081 cgcctgcgcc gcaagatcga gaaggacccg gcgcagccgc gctacatcaa gaccgagcgc
     1141 ggcgcagggt atgtgttcgg tgtccccgtg gagacggttt actgataaag ctgaatcaca
     1201 cgatcgtctc ggcgcgcgac aagcgcgcct cggcggcgtt cctgaccgag atgctcggac
     1261 tgccgcacgc gccggtcgct ttcggcccgt tcatgtgcgt gaaggtggac aacgaagtca
     1321 cgctcgactt ctgcgacgac gacggaagca tcgtttctca gcactacgca ttcctcgtcg
     1381 gcgaaaacga gttcgaccag atcttcggcc gcatcaagag ccgcaagctc gaatactggg
     1441 gcgaccctgc ccgcgccaag cccggcgaga cctacacctg gaacggcggc cgcggcgtgt
     1501 acttcctcga tcccagcggc cacttcctcg aagttcacac ccgcccgtac ttctaattcg
     1561 gggacggagc cctaaccaat tagggctccg tccccgaatt caggaagtgg tcggagagct
     1621 cgaagcggcc acaaatatgg gattgctggc ctgacgcgcg cggcgtagat tggctgcggg
     1681 aggaacccat gatcgccagg atcgccagca cgttttgctg tgtgtcgctg cttgccgcct
     1741 tcgcaccggc cttcgcaact tccgaccacc acgctgccag cattgccggc ctgaaagacc
     1801 gggtgagcgt ggtgcgcgac cgcttcggcg tgccgcacat ctccgcgcgc aacgaccacg
     1861 acgtgtattt catgcagggc tacgtccacg ccggcgaccg cttcttccag atggacgcgt
     1921 cgcgccgcca ggcgagcggc acgctcgccg agctcctcgg gcccggtgcg ctggcgggcg
     1981 acgtgcagct gcgcacgctc ggcctgcgtc gcgccgccgc gcgctcgctg gcggcctacc
     2041 cggccgaggt gcaggcgata ttgcaggcgt acgccgatgg cgtgaacgcc tggctcgcgc
     2101 agggcgcgct gccgccggaa tacgccgcgc tcgagctgac gcgcgttccc gcgtggacca
     2161 cgctcgacac gctcgcggtc ggcaagctcc tcgccttcgg gctgtcgttc gacaccatcg
     2221 accttgacaa cacccgggcg ttgctgacct accagggttt cggcgccgcg ctcggcttcg
     2281 acggcaccaa gctcttcttc gacgacctgt tccgcagcgc accgttcgac ccgacgatct
     2341 cgatcccgga aggcacggtt tccgggccag tgcgcgagcg gcacggccgt ggcgagcacg
     2401 ccgacgggga ctcggtggac aaggcgcgca gctttctcga acgcccgtcg ccgttcttcg
     2461 cgccgcacgg ccgcggcgaa ggcggcagca actggtgggt ggtgagcggc gcgaagaccg
     2521 agtcgcgcaa cccgatgctc gcgagcgatc cgcatctttc gctttcctcg ccgtcggtgt
     2581 tccacgaggt gcatctcgcc gtgcgccgcg gccacaagca ggagccgctc gacgcctacg
     2641 gcgtcggctt ccccggcgtg cccggcgtca tccacggcca caaccagcga atcatgtggg
     2701 gctcgaccgt caacccgatg gacgtcaccg actatttcca ggagcagatc gtgctccagg
     2761 gcggcatccc ggtcgcgacg atgttccgcg gcgcgcccga gccgatcacg atcatcccgg
     2821 aaacctaccg ggcaaacatc gtgggcgacg gcatccagga cagcgtggtg ccgatcacgg
     2881 ccggcgtgcc gccggcgacc ttcgtagtgc cgcgccgtaa caacggcccg ctggtcaccg
     2941 caccggccgc cgacgccagc ggccgcgtca ccgccatcag cgtgcaatac acgggcttca
     3001 gcgcgacgcg cgagatcgag accttcctga gcttcggacg cgccggcagc gtggcggagt
     3061 tcaggcaggc gctgcagttc ttcgacgtcg gatcgcagaa ctgggcttat gccgacgtcg
     3121 acggcaacat cgcgtacttc acgagcgcgg agctgccgtt gcgcgaggac ctgcaggcgg
     3181 gaaccgtcga aggcctgccg ccgtacttcg tgcgcgacgg caccggcgcg ctgcgcaacg
     3241 aatggatccg gaagagcgat ccgcccgcgg accaggcgct cgcctacgag atcctgccgt
     3301 tcgcggagat gccgcagctc gtgaaccccg cgcgcggcta catcgtcaac ggcaacaacg
     3361 acccgatcgg ggtcaccctg gacaacaacc cgctcaaca
//
LOCUS       DATBSZ010000246        12315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_319436, whole genome shotgun sequence.
ACCESSION   DATBSZ010000246 DATBSZ010000000
VERSION     DATBSZ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12315
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319436"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VIG70_15620"
     CDS             <1..613
                     /locus_tag="VIG70_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HEY8252027.1"
                     /translation="AAAAGGFISESVNYLDVGLKLDVEPLIYLDDEIGIKVGLEVSNI
                     VREVKGTGSNTLTYQIGTRNAATSLRLRDGETQILAGLISDEDRRTASRVPGLGEVPV
                     IGRLFSATHDTATRTEVVLLITPRLVRTLARPEARALEFSAGTEASSSGSAPGAFSPG
                     LSLPSSIPEPLSPQPQPARPASTPAPPAGPQLVPFGGFKPPGQ"
     gene            613..1083
                     /locus_tag="VIG70_15625"
     CDS             613..1083
                     /locus_tag="VIG70_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8252028.1"
                     /translation="MRARGFTLIELVTTVAIIAVLASLALPLSELAVQRTKEQELRRA
                     LREIREAIDAYKQAADEGRVVRKPGDSGYPQRLEDLARGIDDQSSPRKERIFFLRRVP
                     RDPFAADSELPAAATWGRRSYASPPDDPRDGDDVFDIYSRAPGIGINGRPYREW"
     gene            1086..1466
                     /locus_tag="VIG70_15630"
     CDS             1086..1466
                     /locus_tag="VIG70_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8252029.1"
                     /translation="MPAARRNGFTLIELLVVLAVIATLLTLALPRYFGSLERSKDAVL
                     KENLHQMREAISLYYADKGRYPETLEALAAEKYLRRVPLDPITDSSVSWIAVAPEDPQ
                     KSGVFDVRSGAPGAGADGIEYARW"
     gene            1460..1936
                     /locus_tag="VIG70_15635"
     CDS             1460..1936
                     /locus_tag="VIG70_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8252030.1"
                     /translation="MVRPRVPGSTAQGFTYLGALFLVALVSGGLAVVGEDWGVAAQRD
                     KEAELLFVGHQYRLAIGRYYRYGPRQYPRSLADLLKDPRRPGTERYLRRLYADPLTAG
                     GEWGLVRAPDGGIQGVYSLSGERPLKTAGFKPRDRELLGAASYTDWKFLHAPQARN"
     gene            complement(2111..2998)
                     /gene="epsA"
                     /locus_tag="VIG70_15640"
     CDS             complement(2111..2998)
                     /gene="epsA"
                     /locus_tag="VIG70_15640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtB/PEP-CTERM-associated transcriptional
                     regulator EpsA"
                     /protein_id="HEY8252031.1"
                     /translation="MTTTAESINPSPAQPAAAFTSMSSMSPGAVAPAEGNAQLPDSAR
                     SAATDFLQIESIVLNLDASLRVHARAHFFSWTQGLLQSLIRHELLICTLCLGKPPAFR
                     ADGFSMTTPDPATMSDVFLRDTAVAPTLLKAWEERRYQPVVFDTSSAPLGSGAFTREM
                     ERLGGTQLLVHGVHDADGRALTFFTFACRPGSIGPRQVYLAQLLAPALHASWVRTQLQ
                     RTPSSADKTSNQGVLTVRELDILKWIYLGKSNIEIGAILSISPLTVKNHVQKILRKLD
                     VLNRTQAVGKALALRILSA"
     gene            3167..4537
                     /locus_tag="VIG70_15645"
     CDS             3167..4537
                     /locus_tag="VIG70_15645"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEY8252032.1"
                     /translation="MRLMRSQPGPGPLVRAALDPLVAIATLVAAVTLFGAGFDGACLI
                     LALLVFAMTFPGGLVRDSEKAGDLMLEITTGWVAVLGLLGLLGWATRTLEAFDQEVIF
                     AWALATPAVLFAAHRLLPLVMPRVLATQGLQKVAVIAGANDLGRRLAERLRDPMLGTR
                     VAGYFDDRGAGRLQNLPAAQNLGDLSRLADFARAQRIDVIYIALPMASQPRILRLLED
                     LRDTTASIYFVPDIFVSDLIQARVDSIGGLPVVAVCESPFYGFNGVVKRVSDIALASL
                     ILLLIAPLLLAIAVGVKLSSSGPILFKQRRYGLDGRKIVVYKFRSMTVAEDGDLVRQA
                     TRNDSRITRFGAFLRRTSLDELPQFINVLQGRMSVVGPRPHAVAHNEMYRKLIRGYMI
                     RHKVRPGVTGLAQVNGFRGETETVEKMKGRIDMDLTYLRNWSLLLDLQIILRTVVVVL
                     GRQNAY"
     gene            4620..4979
                     /locus_tag="VIG70_15650"
     CDS             4620..4979
                     /locus_tag="VIG70_15650"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY8252033.1"
                     /translation="MLGALALLAQIFTLNALPFELVEPWDKVWHFLAYGALTLLLWIA
                     TDGRRPVLVVTAVMLLGAGDELRQALLPARSADALDFAVDVAAAVLTGAALLFTGGRR
                     PGDQAARLAVAKSGLKR"
     gene            complement(4934..5644)
                     /gene="phoB"
                     /locus_tag="VIG70_15655"
     CDS             complement(4934..5644)
                     /gene="phoB"
                     /locus_tag="VIG70_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY8252034.1"
                     /translation="MGGSILVVDDEAAVRDLLSANLQRAGYKVMCAGDVPQAEALVRD
                     TRPDLVLLDWVLPGIPGLTFARRLRSDQRTKDISIVLLSARAEEQDKVTALESGADDY
                     VTKPFSARELLARIKAVMRRRAPQLADDVVEIAGLKIDPAAHQVSAGDKAIDLWTTEL
                     RLLHFFMTHPGRVFSRARLLDEVWGEHVFVEERTVDVHIRRLRQALTPSGHDKLIETV
                     RGTGYRFKPDFATANLAA"
     gene            5796..6140
                     /locus_tag="VIG70_15660"
     CDS             5796..6140
                     /locus_tag="VIG70_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252035.1"
                     /translation="MEDVDFSDEFCRFLQANVPTVEAAELLLLLYRSPEPVVSDPASA
                     KLLQTFQSAGLAAAEGHAVRYRPATPQLEGHVRTLAKAYEERPVTLIRIIYALRDGKI
                     RSFADAFKLRKG"
     gene            6143..6409
                     /locus_tag="VIG70_15665"
     CDS             6143..6409
                     /locus_tag="VIG70_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEY8252036.1"
                     /translation="MAVFNFVLYVLAVLTCLACTVLLFRGYAASGLRLLLWSALCFVF
                     LTVNNLLLFFDLVVFTELDLRPWRLAAALTGLLFLLYGFIFEAE"
     gene            6413..6673
                     /locus_tag="VIG70_15670"
     CDS             6413..6673
                     /locus_tag="VIG70_15670"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEY8252037.1"
                     /translation="MIEFLAGGVTLGYLAAAAFFLSFWRRTSERLFLAFAAAFLLFAI
                     NQGLAFVFTVVSEPASLIYALRVLGFLLLIAAIVDKNRAAKR"
     gene            complement(6670..7359)
                     /locus_tag="VIG70_15675"
     CDS             complement(6670..7359)
                     /locus_tag="VIG70_15675"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY8252038.1"
                     /translation="MRKRRGRGPAWGKLALILVGAAALAAVWRWTPLADFVTAERILG
                     WTRAVRDTWWAPIVLVAAYTPAAFVLLPRPLLTLVSVMTFGVLFGLAYSTTGVLLAAL
                     VTYYCGRALPKETLQRLAGDALEPAGKLLRSHGVLAVFGSNMLPTPPFVVQNMIAGAV
                     RIRLWKFMLGTFLALLPGMLAWTVFGDQINAALEDSSKVSWGLIGGALVLLVAFTFVA
                     RHLLAKRFQSA"
     gene            7412..8482
                     /locus_tag="VIG70_15680"
     CDS             7412..8482
                     /locus_tag="VIG70_15680"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8252039.1"
                     /translation="MQTSERIFYTRAFALIALLLVGYLLYLVLSPFFAPIAWSLFIAF
                     LVYPLHAWLTRRLRGRAALSAALLTLATFLLVIGPLAALGAAFGAQVGDLARGAQAFA
                     AEHKPSDLSDLASLPVVGPAVAWLQQNTGISIAQFQAWAVEGAQAILKGLGTVGRAAF
                     LGALGTVIGFALMMFILFFAIRDGKRMADTVRALVPASDADKARLFRHLASVTRALVY
                     GTGVTALAQGLLVGIGFAIVGLPSPVVFGVLAALAALVPLAGTPVVWVPAMIVLAAQD
                     RWGAALFMLLWGAFVVTIDNFLRPWLVSGRAQIDALTVFIGVLGGVSAFGPIGVFLGP
                     LVLALVIALVRFSLEGQGEERR"
     gene            complement(8479..8850)
                     /locus_tag="VIG70_15685"
     CDS             complement(8479..8850)
                     /locus_tag="VIG70_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8252040.1"
                     /translation="MSEPGRRILIADDNRDWTDGLALLLKDQGYAVRTAYDGREAVGA
                     AREFRPQIVILDVCMPNMTGYEAGRALSALRPGERPVMIAITAWPMESGKLQAELVAG
                     FDHYVGKSADPSNILELLKKV"
     gene            9000..>12315
                     /locus_tag="VIG70_15690"
     CDS             9000..>12315
                     /locus_tag="VIG70_15690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8252041.1"
                     /translation="MKNSRNFLAGGGEMGERMRALDWSKTVLGPVESWPQSLRSAVSI
                     CLGSRFPIVLYWGADYVVLYNDAYAEILGRKHPWALGRRCKEVWSEIWNVIAPMLEGV
                     TVTGEATWSDEQLLILERKGYPEECYFSFSFSPVRGATGGVEGIFTAVIENTRRVLGE
                     RRLRTLRNLAARAAAAKSESEAWRSAGEGLADNPHDLPFAVLYRTDGGGNSIRVAAAG
                     TEPGMESTAWPIERAIASGKMEHAVEHAGGALVLPVSRPGVPAYGCLVAGLGPRRPLD
                     DEYRGFLSLVADQIATAIANARAYEEEKQRAEALAEIDRAKTAFFSNVSHEFRTPLTL
                     MLGPLEDGLNDAEAPLAPAQRERLELVRRNGLRLHKLVNTLLDFSRIEAGRAQASYAP
                     VDLAALTADLASTFRSAVEKAGLGLVVDCPPLSGPAYVDREMWEKIVLNLLSNAFKFT
                     LAGGIQVVLKETENQLRLTVSDTGSGIPADELPHMFERFHRVEDARGRSHEGSGIGLA
                     LVNELVKLHGGSISVDSRPGEGATFTVALPKGSAHLPAERVKAPRAAASSTAARAGAY
                     VAEALGWLPGVQTAPAQPQSAHRILLADDNADLREYARRLLAEHYDVEVAADGRAALE
                     AARARRPELIISDVMMPHLDGFGLIRELRADPELAAIPVILLSARAGEEARLEGLGRG
                     ADDYLVKPFSPRELLVRVGTLVKSAEMHRQAREARAQFETLLNQAPVGVYLVDQDFRI
                     AAVNPVAAPAFGDIPGLIGRDFDEVIHILWPKAYADEVVRIFRRTLETGEPHVVPERI
                     EQRLDRGVTEYYEWQVNRIPLPGNRRGAVCYFRDISKSVMAREALREADSRKDEFIAT
                     LAHELRNPLAPLRSSLDTLKLAATQGVPPGQPLEIMERQLSHLVRLVDDLMEVSRITR
                     GNFELRKEPVRLDVALRSAVEACDPLIRAGDHRLNVSLPSEPIVLDADPVRLAQVFGN
                     LLNNAAKYSEGAGTISVEARREALEAVVVVADTGEGIPPEQLPQLFKIFSRGSHSSRR
                     NQSGLGIGLALVRRLVEMHGGRVEAESEGRGKGSRFIVRLPLNAAQQLAPAAIKLERP
                     AVAPMTILIVDDNEDAA"
BASE COUNT         1868 a         4252 c         4187 g         2008 t
ORIGIN      
        1 cgcggcggcc gcgggggggt tcatatccga gtcggtgaac tacctcgatg tcggcctgaa
       61 gctcgacgtc gagccgctga tctacctcga cgatgagatc ggcatcaagg tcggcctgga
      121 ggtgagcaac atcgtgcgcg aggtgaaggg caccggcagc aacacgctga cctaccagat
      181 cggcacgcgc aacgcggcca cctcgctgcg cctgcgcgac ggcgaaacgc agatcctcgc
      241 cgggctgatc agcgacgagg accggcgcac cgccagccgc gtgccggggc tgggcgaagt
      301 tccggtcatc gggcgcctct tctccgccac gcatgacacg gcaaccagga ccgaggtggt
      361 gctgctgatc acgccgcggc tggtgcgcac gctggcgcgg ccggaggcgc gcgcgctcga
      421 attctcggcc ggcaccgagg cgtccagcag cggctcggcg ccgggcgcgt tctcccccgg
      481 actgtcgctg ccctcttcaa tccccgagcc gctctcgccg cagccccagc cggccagacc
      541 ggcaagcacg ccggcgccgc cggccgggcc gcagctggtg ccgttcggcg gcttcaagcc
      601 gcccgggcag tgatgcgcgc acgcggcttc actctgatcg agctggtgac cacggtcgcg
      661 atcattgccg tgctcgccag cctcgccctc cccttgagcg aactggcggt gcagcgcacg
      721 aaggagcagg agctgcgccg cgcgctgcgc gagatccgcg aggcgatcga cgcgtacaag
      781 caggccgccg acgaaggccg cgtggtcagg aaaccgggcg actccggcta cccgcagcgg
      841 ctggaggacc tggcgcgcgg catcgatgac cagagcagcc cgcgcaagga acgcatcttc
      901 ttcctgcgcc gcgtgccgcg cgaccccttc gccgcggatt ccgagctgcc ggccgccgcg
      961 acctggggcc ggcgcagcta cgccagcccg cccgacgatc ccagggacgg cgacgatgtg
     1021 ttcgacatct actcgcgcgc gccgggcatc ggcatcaacg gccggccgta ccgggaatgg
     1081 tgacgatgcc cgcggcgcgg cgcaacggtt tcacgctcat cgaactgctg gtggtgctcg
     1141 cggtcatcgc caccctcctc acgctcgccc tgccgcgcta cttcggcagc ctcgagcgct
     1201 cgaaggacgc ggtgctgaag gaaaacctgc accagatgcg agaggcgatc tcgctctact
     1261 acgccgacaa aggcaggtac ccggaaacgc tcgaggccct ggcggcggaa aagtacctgc
     1321 gcagggtgcc tctcgacccg atcaccgaca gcagtgtgag ctggatcgcc gtggcgccgg
     1381 aggacccgca aaagagcggc gttttcgatg tgcgcagcgg cgcgcccggc gcaggcgccg
     1441 acggcatcga gtacgctcga tggtgaggcc gcgcgtaccg ggctccaccg cgcagggttt
     1501 cacctacctg ggggcgctgt tcctggtcgc cctcgtgtcg ggcggcctgg ccgtggtcgg
     1561 ggaggactgg ggggtcgctg cacagcgcga caaggaggcc gaactgctgt tcgtcggcca
     1621 ccagtaccgc ctcgcaatcg ggcgttacta ccgttacgga ccgcgccagt atccgcgctc
     1681 gctcgcggat ctgctgaagg atccgcgccg gcccggcacg gagcgctacc tgcgccgcct
     1741 ctatgccgat ccgctcaccg ctggcggcga atggggcctc gtacgggcgc cggacggcgg
     1801 aatccagggg gtctacagcc tgtccgggga aaggccgctc aaaaccgcgg gattcaagcc
     1861 gcgggaccgt gaacttctgg gcgcggcaag ctatacggac tggaaattcc tgcacgcgcc
     1921 gcaggcacgc aattgaatgt cgccgtatca ctacttgctc ttgtcgaaac gcgacagcag
     1981 gttcggggag gcccgatagc gaagccgcgc accgggaagt attctggagg cgatagatgt
     2041 ccctcttcag acgttccccc gaagcatcta tcaggcccgg acctaacccc tccgggcttt
     2101 tttttggtct ttaggcgctc aggatgcgca gcgcgagcgc cttgccgacc gcctgggtgc
     2161 ggttcagcac gtcgagcttg cgcaggatct tctgcacgtg gttcttcacg gtcagcgggc
     2221 tgatgctgag aatggccccg atctcgatat tgctcttgcc gaggtagatc cacttcagga
     2281 tgtcgagctc gcgcaccgtc agcacgccct ggttcgaggt cttgtcggcc gagctcggcg
     2341 tgcgctgcag ctgcgtgcgc acccaggagg cgtgcagcgc cggcgcgagg agctgcgcga
     2401 gatagacctg gcgcggcccg atgctgccgg ggcggcaggc gaaggtgaag aacgtcagcg
     2461 cgcgcccgtc ggcgtcgtgc acgccgtgca ccaggagctg cgtgccgccg aggcgctcca
     2521 tttcgcgggt aaatgctcca ctacccaagg gagcactcga agtatcaaaa accaccggtt
     2581 ggtagcgccg ctcctcccag gccttgagca gcgtcggcgc gaccgcggtg tcgcgcagga
     2641 agacgtcgga catcgtcgcc ggatcgggcg tcgtcatgga gaagccgtcg gcgcggaacg
     2701 ccggcggctt gcccaggcag agcgtgcaga tcaggagctc gtgccggatc aggctctgca
     2761 gcaggccctg cgtccagctg aagaagtgag cgcgggcgtg cacgcgcagc gaggcgtcga
     2821 ggttcagcac gatcgactcg atttgcagga agtcggtggc cgccgagcgc gccgaatccg
     2881 gcaactgcgc attaccctcc gccggggcga ctgcccccgg gctcatcgag ctcattgagg
     2941 tgaaggcggc ggcgggctgc gccggcgatg ggttgatgct ctctgcagtc gtggtcattg
     3001 gcatggctct cgggaatata gtcggttgag attacgcaat agttcgtcgg gagcatcggc
     3061 gaaacgctag tccggtgggc ctacgcggcg gcctcgcgcg ccacgtcacg gttgaaccgt
     3121 gctcgcctac cctgtcttgg ccttgcacgc aacgcccgaa acccggatga gactcatgcg
     3181 cagccagccc ggccccgggc cgctggtgcg ggctgcgctc gatccgctgg tggcgatcgc
     3241 cactctcgtg gcggcggtta cgctgttcgg cgccgggttc gacggcgcct gcctgatcct
     3301 cgcgctgctg gtcttcgcga tgacctttcc cggcgggctg gtgcgcgaca gcgaaaaagc
     3361 cggagacctg atgctcgaga tcacgaccgg ctgggtcgcg gtcctcggcc tgctcggcct
     3421 gctcggctgg gccacgcgca cgctggaagc gttcgaccag gaggtgatct tcgcctgggc
     3481 gctcgccacg cccgcggtcc tgttcgccgc gcaccggctg ctgccgctgg tgatgccgcg
     3541 cgtgctcgcc acgcaggggc tgcagaaggt cgcggtgatc gcgggcgcca acgacctcgg
     3601 ccggcggctc gccgagcggt tgcgcgaccc gatgctcggc acccgcgtcg cgggctattt
     3661 cgacgaccgc ggcgccgggc gcctgcagaa cctgccggcg gcgcagaacc tgggcgatct
     3721 ctcgcgcctc gccgacttcg cgcgcgcgca gcgcatcgac gtgatctaca tcgcgctgcc
     3781 gatggcgtcg cagccgcgca tcctgcgcct gctcgaggac ctgcgcgaca ccaccgcgtc
     3841 gatctacttc gtgcccgaca tcttcgtctc cgacctgatc caggcgcgcg tcgactcgat
     3901 cggcggcctg ccggtggtgg cggtgtgcga gagcccgttc tacggcttca acggcgtggt
     3961 caagcgcgtg agcgacatcg ccctggcaag cctgatcctt cttctcatcg cgccgctgct
     4021 gcttgccatc gcggtcggcg tgaagctctc ttcgtccggg ccgatcctgt tcaagcagcg
     4081 ccgctacggc ctcgacggca ggaagatcgt ggtctacaag ttccgctcga tgacggtggc
     4141 cgaggacggc gacctggtgc gccaggcgac gcgcaacgac agccggatca cgaggttcgg
     4201 cgccttcctg cgccgcacct cgctcgacga gctgccgcag ttcatcaacg tgctgcaggg
     4261 acgcatgagc gtggtcggcc cgcggccgca cgcggtggcg cacaacgaga tgtaccgcaa
     4321 gctgatccgc ggctacatga tccgccacaa ggtgcgcccg ggggtcaccg gcctcgccca
     4381 ggtgaacggc tttcgcggcg agaccgagac cgtcgagaag atgaaggggc gcatcgacat
     4441 ggacctgacc tacctgcgca actggtcgct gctcctcgac ctgcagatca tcctgaggac
     4501 cgtggtcgtc gtgctcggaa ggcagaatgc ctactgacgg aattcgggga cggagcccta
     4561 actcccatag cgggagcgga gattccggag ttagggctcc gtccccgaat tcctgggcgt
     4621 tgctcggggc gctcgcgctg ctggcgcaga tcttcacgct gaacgcgttg ccgttcgagc
     4681 tggtcgagcc gtgggacaag gtgtggcact ttctcgcgta cggggcgctc acgctgctcc
     4741 tgtggatcgc gactgacggc cgccgcccgg tgctggtggt gacggcagtg atgctgctcg
     4801 gcgccggcga cgagctgcgc caggcactgc tgcctgcacg cagcgccgac gcgctcgatt
     4861 ttgccgtcga cgtggctgcg gcggtgctca ccggtgcggc gctcctcttc acgggaggaa
     4921 ggcgcccggg cgatcaggcc gcgaggttgg ccgtcgcgaa gtcgggcttg aagcggtagc
     4981 cggtaccgcg caccgtttcg atcagcttgt cgtggccgct cggcgtgagc gcctggcgca
     5041 gcctgcggat gtgcacgtcc accgtgcgct cctccacgaa tacgtgctcg ccccagacct
     5101 cgtccaacag cctggccctg gagaagacgc ggccgggatg ggtcatgaag aagtgcagca
     5161 ggcgaagctc cgtggtccac aggtcgatcg ccttgtcgcc ggcgctgacc tggtgcgcgg
     5221 ccggatcaat cttgaggccc gcgatctcga ccacgtcgtc ggcgagctgt ggcgcgcgcc
     5281 ggcgcatgac tgctttgatc ctggccagca actcccgggc tgaaaacggc ttggtgacgt
     5341 agtcgtcggc gccgctctcc agcgcggtga ccttgtcctg ctcttccgcc cgcgcggaca
     5401 gcaggacgat cgagatgtcc ttggtccgct ggtcggagcg cagccgccgt gcgaaagtca
     5461 gcccgggaat tcccgggagc acccagtcga ggagcaccag gtcgggacgc gtgtcgcgca
     5521 ccagcgcctc ggcctgcggc acatcgccgg cgcacatcac cttgtagccg gcgcgctgca
     5581 ggttggcgga cagcagatcg cggaccgccg cttcgtcgtc aactaccagt atcgaaccgc
     5641 ccatggccac tcccgttgtt tagaggcgaa gccatgctat gtcgcggttc ggcgtaggca
     5701 tatcgccgga cccttattta acagtcgcgt catattggcc gggtgtcgtt actcaccgac
     5761 agaccggaca gaccgctccg tgctagataa gggccatgga agacgtggat ttttcagacg
     5821 aattctgccg cttcctgcag gcgaacgtcc ctaccgtcga ggccgccgag ctgctgcttc
     5881 tgctctatcg cagccccgag ccggtggtaa gcgatccggc ctcggcgaaa ctcctgcaga
     5941 cgttccagtc ggcggggctg gcggctgccg aggggcacgc cgtgcgctac cgcccggcca
     6001 cgccgcagct cgagggccac gtccgcacgc tcgccaaggc gtacgaggaa aggccggtca
     6061 cgctgatccg catcatctac gcgctgcgcg acggcaagat ccgctctttc gccgacgcat
     6121 tcaagctgag gaagggctag ccatggccgt gttcaacttc gtgctgtacg tccttgccgt
     6181 gctcacctgc ctcgcatgca cggtgctgct cttccgcggc tacgccgcga gcggcctgcg
     6241 gctgctcctg tggagcgcgc tgtgcttcgt gtttctcacc gtaaacaacc tgctcctctt
     6301 cttcgacctg gtggtgttca ccgagctcga cctgcgcccc tggcggctcg ccgcggcgct
     6361 caccggattg ttgttcctcc tttacggatt catctttgaa gcggagtagc ccatgatcga
     6421 attcctcgcc ggcggggtca cgctgggcta cctggcggcc gcggccttct tcctctcgtt
     6481 ctggcgcagg acctcggagc gcctgttcct cgccttcgcc gccgcgttcc tgctgttcgc
     6541 gatcaaccag gggctcgctt tcgtcttcac cgtggtgagc gagccggcga gcctgatcta
     6601 cgccctgcgc gtgctgggtt tcctcctcct catcgccgcg atcgtggaca agaaccgcgc
     6661 cgcgaagcgc taggccgatt gaaagcgttt tgccagaagg tgccgggcga cgaaagtaaa
     6721 agcgaccagc aggacgagcg cgccgccgat gaggccccag ctcaccttgg aggagtcctc
     6781 gagcgccgcg ttgatctggt cgccgaacac cgtccaggcg agcatccccg gcagcagcgc
     6841 gagaaaggtg ccgagcatga atttccacag ccggatgcgc accgccccgg cgatcatgtt
     6901 ctgcaccacg aacggcggcg tcggcagcat gttggagccg aacaccgcga ggacgccgtg
     6961 gctgcgcagc agcttgcccg cgggctccag cgcgtcgccg gcgaggcgct gcagcgtctc
     7021 cttcggcagc gcgcgcccgc agtaataggt gacgagcgcg gcgagcagca cgccggtggt
     7081 gctgtaagcg agcccgaaca gcaccccgaa ggtcatcacc gacaccagcg tgaggagcgg
     7141 gcgcggcagc agcacgaagg ccgcaggcgt atacgcggcc accagcacga tcggcgccca
     7201 ccaggtgtcg cgcaccgcgc gcgtccagcc gaggatgcgc tcggcggtca cgaagtcggc
     7261 aagcggcgtc cagcgccaca ccgccgcgag cgcagcggcg ccgacgagaa tcagcgcgag
     7321 cttgccccag gcggggccgc ggccgcggcg tttgcgcatc gccggcagtc tatttggtgc
     7381 aattctcttt tgcaggcagg atagataggc catgcagaca tccgagcgca tcttctacac
     7441 gcgcgccttc gcgctcatcg cgctgctgct ggtcggctat ctgctgtacc tcgtgctctc
     7501 gccgtttttc gcgccgatcg cgtggtcgct gttcatcgcc ttcctcgtct acccgctgca
     7561 cgcatggctt acgcgcaggc tgcgcggacg cgccgccctc tccgccgccc tgctcaccct
     7621 ggcgactttc ctgctggtga tcggccccct cgcggcgctc ggcgcggcat tcggcgcgca
     7681 ggtgggcgac ctcgcgcgcg gagcgcaggc cttcgccgcc gagcacaagc cttcggacct
     7741 gtccgacctg gcgagcctgc cggtcgtcgg gcccgcggtg gcctggctgc agcagaacac
     7801 cggcatctcg atcgcgcagt tccaggcctg ggccgtcgag ggcgcgcagg cgatcctcaa
     7861 ggggctgggc acggtcggac gcgccgcgtt cctcggcgcg ctcggcacgg tgatcggctt
     7921 cgccctgatg atgttcatcc ttttcttcgc catccgcgac ggcaagcgga tggccgacac
     7981 ggtgcgcgcc ctggtgcccg cgtccgacgc cgacaaggcg aggctgttcc ggcacctcgc
     8041 ctcggtcacg cgcgcgctgg tctacggcac gggggtcacc gcgctggcgc agggactgct
     8101 ggtcgggatc ggattcgcga tcgtgggcct gccctccccg gtggtgttcg gcgtgctcgc
     8161 cgcgctcgcc gcgctcgtgc cgctcgccgg cacgccggtg gtgtgggtgc cggcgatgat
     8221 cgtgctcgcg gcgcaggacc gctggggcgc ggcgctcttc atgctgctgt ggggcgcctt
     8281 cgtggtgacg atcgacaatt tcctgcgccc gtggctggtg tcgggccgcg cccagatcga
     8341 cgcgctgacg gtgttcatcg gcgtgctggg cggagtctcg gcgttcggcc cgatcggcgt
     8401 gttcctcggg ccgctggtgc tcgcgctggt gatcgccctg gtgcgcttct ccctggaagg
     8461 acagggcgag gagcggcgct agaccttctt cagcaactcc aggatgttcg acgggtccgc
     8521 ggacttgccg acgtagtggt cgaaccccgc caccagctcg gcctggagct tgccggattc
     8581 catcggccac gcggtgatcg cgatcattac ggggcgctcg cccggacgca gcgcgctcag
     8641 cgcgcgcccg gcctcgtagc cggtcatgtt gggcatgcac acgtcgagga tcacgatctg
     8701 cggccggaac tcgcgcgccg cgccgaccgc ctcccttcca tcgtaagcgg tgcgcacggc
     8761 gtagccctgg tccttcagca gcagcgcgag gccgtcggtc cagtcgcggt tgtcgtccgc
     8821 gatgagaatg cgcctgccgg gctcgctcac gggcggatca tcctcagcag ctcgacgcag
     8881 ttggcagcca tcacgcggta acttacgctc gtcgtgacgc ggactcaata gccgcgtgta
     8941 gtggaattac cgacgttacg tgccttgccg gtcctcctag aatcccgggg tctgactcca
     9001 tgaagaatag ccggaacttc cttgcgggcg gcggcgagat gggcgagcgc atgcgcgccc
     9061 tcgactggtc gaagaccgtg ctcggcccgg tcgagagttg gccgcagagc ctccgctccg
     9121 cggtcagcat ctgcctgggc tcgcgcttcc cgatcgtcct ttactggggc gcggactacg
     9181 tcgtgctgta caacgacgcc tacgccgaga tcctcggcag gaagcacccg tgggcgctcg
     9241 gtcggcgctg caaggaggtg tggtcggaga tctggaatgt gatcgcgccg atgctggaag
     9301 gtgtaacggt gacgggcgaa gcgacgtggt ccgacgaaca gctcctgatc ctcgagcgca
     9361 aaggctaccc ggaggaatgc tacttctcct tctcgttcag cccggtccgc ggcgcgaccg
     9421 gtggggtcga gggcatcttc accgccgtca tcgaaaatac gcggcgggtg ctcggcgagc
     9481 gccgtctgcg aacgctgcgc aacctggcgg cacgcgccgc cgctgccaag agcgaaagcg
     9541 aggcatggcg aagcgccggc gaaggtctcg ccgacaaccc gcacgacctg cccttcgccg
     9601 ttctctaccg aaccgacggc gggggcaatt cgattcgcgt cgccgccgcg ggtacggagc
     9661 cgggcatgga atcgacggcc tggccgatcg agcgggcgat cgccagcggg aaaatggaac
     9721 acgctgtgga acacgctggc ggcgcgctgg tgctgccggt ctcccgaccc ggcgtgccgg
     9781 cgtatggatg cctcgtcgcg ggcctgggcc cgcgacggcc gctggacgac gaataccgcg
     9841 gattcctgtc tctcgtcgcc gaccagatcg ctacggccat cgccaatgcc cgtgcgtacg
     9901 aggaagagaa acagcgagcc gaggcgctgg ccgagatcga ccgcgcgaag accgcgttct
     9961 tctcgaacgt gagccacgag ttccgcacgc ccctcacgct gatgctcggc ccgctcgagg
    10021 acggcctgaa cgatgccgag gcgccgcttg cccccgcgca gcgcgagcgc ctggagctcg
    10081 tgcgccgcaa cgggctgcgc ctgcacaagc tggtcaacac gctgctcgac ttttcgcgca
    10141 tcgaagccgg ccgcgcgcag gcgagctatg cgccggtcga tctcgccgcc ctcaccgccg
    10201 atctcgcctc gaccttccgc tccgcggtgg agaaagccgg cctcgggctc gtcgtcgact
    10261 gcccgccgct ctccgggccg gcctacgtcg accgcgagat gtgggaaaag atcgtcctca
    10321 acctgctttc caacgccttc aagttcacgc tcgccggagg aattcaggtt gtcctgaagg
    10381 aaaccgaaaa tcaattacgg ctcaccgtga gcgataccgg gagcggcatt ccggccgacg
    10441 agctgccgca catgttcgag cgttttcatc gcgtggagga cgcccgcggc cgcagccacg
    10501 agggaagcgg catcggcctc gcgctggtta acgagctggt caagctgcac ggcggatcga
    10561 tcagcgtgga cagcaggccg ggcgaaggcg ccacgttcac ggtcgcgctg ccgaaaggca
    10621 gcgcgcacct tccggccgag cgggtcaagg cgccgcgcgc agccgcgagc tccaccgccg
    10681 cacgggccgg cgcctacgtc gccgaggcgc ttggctggct gcccggagtc cagaccgcgc
    10741 cggcgcagcc gcagagcgcg caccgcattc ttctcgcgga cgacaacgcc gacttgcgcg
    10801 agtacgcgcg caggctgctg gcggagcact acgacgtcga ggtcgcggcc gacggccgcg
    10861 ccgcgctcga ggcggcgcgc gcacggcgtc ccgaactcat catctcggac gtcatgatgc
    10921 cgcacctcga cggcttcggt ctgatccgcg agctgcgcgc cgatccggag ctggccgcga
    10981 ttccggtgat cctgctctcc gcgcgcgcgg gcgaagaagc gcgcctcgag gggctgggcc
    11041 ggggtgccga cgactatctg gtgaagccct tcagcccgcg cgagctgctg gtccgggtcg
    11101 gtacgctggt gaaatcggcc gagatgcacc gccaggcgag agaggcacgc gcgcagttcg
    11161 agaccctcct caaccaggca ccggtcggcg tgtatctcgt cgaccaggac ttccgcattg
    11221 ctgcggtgaa ccccgtggcg gcgcccgctt tcggcgacat tcccggcctc atcggtcgcg
    11281 atttcgacga ggtgatccac atcctctggc ccaaggccta tgccgacgag gtggtacgca
    11341 tcttccgccg cacgctcgag accggggagc cgcacgtcgt gcccgagcgc atcgagcagc
    11401 gcctggaccg cggggtcacc gagtactacg aatggcaggt gaaccgcatt ccgcttcccg
    11461 gcaaccggcg cggcgcggtg tgctacttcc gcgacatctc gaaatcagtc atggcgcgcg
    11521 aggcactgcg cgaggccgac agccgcaagg acgagttcat cgctacgctg gcgcacgagc
    11581 tgcgcaaccc gctcgctccg ctgcgcagct cgctcgacac gctgaagctc gccgcaacgc
    11641 agggcgttcc ccccggccag ccgctcgaga tcatggagcg ccagctctcc cacctggtgc
    11701 gcctggtcga cgacctgatg gaggtctcgc gcatcacccg cggcaacttc gagctgcgca
    11761 aggagccggt gcggctggac gtcgcgctgc gcagcgccgt cgaggcctgc gatcccctga
    11821 tccgcgccgg cgaccatcgc ctcaacgtct cgctgccgag cgagccgatc gtgctcgacg
    11881 ccgacccggt gcgcctggcg caggtgtttg gcaacctcct caacaacgcc gcaaagtact
    11941 ccgagggcgc cggcacgatc agcgtcgaag cgcggcgcga ggcgcttgaa gccgtcgtgg
    12001 tcgtcgccga cacgggcgaa ggcatcccgc ccgagcagct gccgcagctc ttcaagatct
    12061 tttcccgtgg cagccacagc agccggcgca atcagagcgg cctgggtatc ggcctcgctc
    12121 tggtgcggcg cctggtcgag atgcacggcg ggcgggtcga ggcggaaagc gaaggccggg
    12181 gcaagggcag ccgcttcatc gttcgcctgc ccctgaacgc ggcacagcag cttgctcccg
    12241 ccgcgatcaa gctcgagcgg cccgcggtcg cgccgatgac catcctcatc gtggacgaca
    12301 acgaggatgc cgccg
//
LOCUS       DATBSZ010000247         6929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_387784, whole genome shotgun sequence.
ACCESSION   DATBSZ010000247 DATBSZ010000000
VERSION     DATBSZ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387784"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VIG70_15695"
     CDS             complement(<1..203)
                     /locus_tag="VIG70_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252042.1"
                     /translation="MSAHGSAEGVLPHAVGEQAVEVAKPGMVYARMFSRGSLSSSIVR
                     AQTISAWVESRPPETPMISFFEPV"
     gene            138..1283
                     /locus_tag="VIG70_15700"
                     /pseudo
     CDS             138..1283
                     /locus_tag="VIG70_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158935.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
     gene            complement(1194..2141)
                     /locus_tag="VIG70_15705"
     CDS             complement(1194..2141)
                     /locus_tag="VIG70_15705"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8252043.1"
                     /translation="MSAELTVVSPDGASHVAQVFLPERLDAPVVLCLPAMGAAADYYQ
                     PFAEGIARAGAGVAALLDVRGQGRSSAAARRGDDFGYREILELDMPAAISQLRRVFPG
                     RPVYLAGHSLGGQLALLFAARNPSVLAGLVLIAAGTTHYRAWPGYKRALTWLYLSGIR
                     VAAATLPWYPGTRLGFGGDQPRRLMRDWGRVVQRGSYRPEGSAFDYEAAARELSLPIL
                     ALGVREDPVAPPSALEALLAKAPRAPVTRVEVPGVLAHRPWKRHFSWAREPADVVAAL
                     GAWLAEHAEHLLHRNSGEVPQPGIGNSVRRRAPVAAGRE"
     gene            complement(2138..2863)
                     /locus_tag="VIG70_15710"
     CDS             complement(2138..2863)
                     /locus_tag="VIG70_15710"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY8252044.1"
                     /translation="MLLEWIDYYSLLFLAPFLALEFFWRARGYEAPRGWRWRAGAVSL
                     AVFGVSFAVGRLWGTVLPQWSLVDGAALGTWGGAIAGILVYELMHYWYHRLAHQWNWL
                     WRLGHQMHHSAESVDAFGAYYLHPLDAALFTTWAVIAFYPLLGLTPEAGAIATAFLAF
                     NAAFQHANIRTPRWLGYLVQRPESHGIHHGRGIHRWNYADLPLWDMVFGTFRNPVEAK
                     REAGFYTGASAKLGSMLAFREVA"
     gene            3024..3230
                     /locus_tag="VIG70_15715"
     CDS             3024..3230
                     /locus_tag="VIG70_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252045.1"
                     /translation="MSPNAPHLKALLNMAPSAFACGDIGKLPAIKSLAALRKEVQGHF
                     GKLSVQEIDALVGWFNRNFFRLDD"
     gene            complement(3301..3477)
                     /locus_tag="VIG70_15720"
     CDS             complement(3301..3477)
                     /locus_tag="VIG70_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252046.1"
                     /translation="MKRKYGAALARETLAEQMMRERAAEMKDNRFNFGSIAKKGSPTR
                     GKRRGRAFKPAHRR"
     gene            complement(3483..4055)
                     /gene="lpoB"
                     /locus_tag="VIG70_15725"
     CDS             complement(3483..4055)
                     /gene="lpoB"
                     /locus_tag="VIG70_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HEY8252047.1"
                     /translation="MKAHLVIALAAAALMGGCAKEQTRYGDARGVETMTNQFGSTDLQ
                     LMAESMARSMLQAPVIASSNLPVVTVQEVRNKTSEYIDTRAITDSIRSELQKGGRVRF
                     AVDQAGMNPQVDELKRQQSGLYAKDTAAEMGQMVGAQYRLEGNIISIVKQVKDAKDVY
                     YKLNLQLWNIRNGLLEWSDEKEIRKTTTRG"
     gene            complement(4052..4486)
                     /locus_tag="VIG70_15730"
     CDS             complement(4052..4486)
                     /locus_tag="VIG70_15730"
                     /inference="COORDINATES: protein motif:HMM:NF018889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HEY8252048.1"
                     /translation="MNPKWMIAVAASAMLAAGCATKKGFEQPGFAFGCPIGEAKVSSM
                     DDLVKAKVLAEGRRTTIQTTEMRCSKSGELWKIDANLNNDSKSVKRIAYKFRWIDQQG
                     MRAWEEEPWKPLMLYENSNLVINTISPTNKAADFRLILRSEE"
     gene            complement(4483..5829)
                     /locus_tag="VIG70_15735"
     CDS             complement(4483..5829)
                     /locus_tag="VIG70_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252049.1"
                     /translation="MVLLLSGCAAMRSYDAELYTTLDRASSGSVDGAIQLLESNNRLP
                     DKDLLYYLELGMLQRLGGRYAESQKAWAAANARIQATTEGGLAQMSNLMRGMSSYVIN
                     DKLRAYSGHDYEKVMLLTYMALNHLAMGDYDSARVAIKQAHELEAQIAELHTRQYAEV
                     EESARQRGAHTSFKELNGYPVETIDTPEVNALRNAYQSALSHYLAGFIYEALGEPSLA
                     APGYRLANELQPGQPLLEEALRGLDQRVAAPDDGMTEVLVIIGTGSAPALQSRQFALP
                     VVVNNKLLFLSNAFPVMTTTSWEPLPTQVTLEEHVLPATRIASVDLMARRRLKDDMPG
                     IMLRATIRSTVSATLQYQAQRAGDREGTLALALAAGIVAVGSVALASADDRTWRALPS
                     DVSIARGRLPRGTRTITLHTPQGARSAAVSLSGRYAVVDLRLLRHQLFVNGPREAR"
     gene            complement(5841..6680)
                     /locus_tag="VIG70_15740"
     CDS             complement(5841..6680)
                     /locus_tag="VIG70_15740"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY8252050.1"
                     /translation="MRRSAFALLIVLAGCTTSPLTGRSQYMVVSEQMAVSQSAAAYVQ
                     MMGELDKKKKIEAGTEREKRVREITDRLVAQAVRVRPDAKSWNWEVKVIDDPKTVNAF
                     CMAGGKMAIYSGMWEKLKATDDEIAQVMGHEIAHALADHTRERMSIAMTSAVATTAAA
                     IAIGSRDSGPGAGLALTGAQLAAVLAIQLPNSRDSESEADQIGIELAARAGYDPKAAV
                     TLWDKMGKEGGGPPEFLSTHPTPQNRSARLAELGRQVTPLYLAAKAGTASDSDKLITF
                     PRK"
BASE COUNT         1083 a         2361 c         2309 g         1176 t
ORIGIN      
        1 accggctcga agaagctgat catcggtgtc tccggtggcc tcgactccac ccaggcgctg
       61 atcgtctgtg cgcgcacgat ggacgagctc aagctgccgc gcgagaacat cctggcgtac
      121 accatgccgg gcttcgcgac ctcgaccgcc tgctcgccga ccgcatgcgg cagaacacct
      181 tcggcgcttc catgcgccga catgcggaca aggtgttccg caccatcgag gtcccggtgc
      241 cgctgcccgg aggaaaactc ctcctgaaga gaaagctgga acaaaagccc tacgtgccga
      301 gcgacccgcg tacgcgcgac gcgcgctgcg aggaggtcta ccgcatccag gtacagggcc
      361 tggcgacccg catgcgcgcc accggcacca agaagctggt gatcggcatc tcgggcggcc
      421 tcgattcgac gcaggccctg atcgtctgcg cgcgcgccat ggaccagctc aagctgccgc
      481 gcgagaacat cctggcttac accatgccgg gcttcgccac ctcgacgcgc accaagagcc
      541 aggcctggca gctgatgcgc gccgtgggcg cgagcgccga ggagatcgac atccggccct
      601 catgcctgca gatgctgaag gacatcggcc acccgtacgc caagggcagg aaggtctacg
      661 acatcacctt cgagaacgtg caggcgggcg agcgcaccag ccacctcttc cgcctcgcca
      721 acctgcacgg cggcttcgtg gtcggcaccg gcgacctgtc cgagctcgcg ctcggctggg
      781 cgacctacgg cgtcggcgac cacatgtcgc actacaacgt gaacgcgagc gtgcccaaga
      841 ccctgatcca gcacctcatc ggctggatcg cggagtcgga ggaattcagc gtcgaggtga
      901 agcgcgcgct gaagcgcgtg ctcgagaccg agatcagccc ggagctggtg cccggcaagc
      961 agtcgagcga ggcatcggtg ggaccctacg agctgcagga cttccacctc tattacctgc
     1021 tgcgtttcgg ctacgccccc accaagatcg cgttcctcgc ctggcacgcc tggcgcggca
     1081 agtaccggct ggccgcgatc cgcaagtggc tcggcgtgtt cctcgagcgc ttcttccgca
     1141 accagtacaa gcgcagtgcg gtgccgaacg cgcccaaggt cggctcgggc ggctcactct
     1201 cgccccgcgg cgactggcgc gcgccgtcgg acgctgttgc caatgcctgg ctgagggacc
     1261 tctccagaat tcctgtgaag tagatgttcg gcgtgctcgg cgagccacgc gccgagcgcc
     1321 gcgaccacgt ccgcgggctc gcgcgcccag gagaagtggc gcttccaagg acggtgggcg
     1381 agcacgcccg gcacttcgac gcgcgtgacc ggcgcgcgcg gcgccttggc gagcagcgct
     1441 tcgagagccg agggcggcgc gaccgggtcc tcgcgcacgc cgagcgccag gatcggcagc
     1501 gaaagctcgc gcgccgcggc ctcgtagtcg aacgccgatc cctcgggccg atagctgccg
     1561 cgctgcacta cgcgtcccca gtcgcgcatc agccggcgcg gctggtcgcc gccgaagccg
     1621 aggcgagtcc ccgggtacca cggcagcgta gctgccgcga cccggatccc ggagagatag
     1681 agccaggtca gggcgcgctt gtagccaggc cacgcgcgat agtgcgtcgt gcccgcggcg
     1741 atcaggacaa ggccggcgag tacgctggga ttgcgcgcgg cgaagagaag cgcaagctgc
     1801 ccgcccaggc tgtggcccgc aagatagacc gggcgccccg gaaagacgcg ccgcagctgg
     1861 gagattgccg ccggcatatc gagctccagg atttcgcggt agccgaagtc gtcgccgcgg
     1921 cgcgcggccg cgctgctgcg accctggccg cgcacatcga ggagcgccgc aacgccagcg
     1981 ccggcccgcg cgattccttc ggcgaatggc tggtagtagt cggcggcggc acccatcgcc
     2041 ggcaggcaaa gcacgaccgg cgcgtccagc cgctccggca ggaagacctg cgcgacgtgg
     2101 cttgctccat cgggggagac gacggtgagc tccgcgctca tgcaacttcc cggaacgcca
     2161 gcatggagcc gagcttcgcc gaggcgccgg tgtagaaccc ggcttcgcgc ttcgcctcga
     2221 ccgggttgcg gaaggttccg aacaccatgt cccacagcgg caggtcggcg tagttccagc
     2281 ggtgaatgcc gcggccgtgg tggatgccgt ggctctcggg gcgctgcacg aggtaaccga
     2341 gccagcgcgg cgtgcggata ttggcgtgct ggaaggcggc gttgaaggcg aggaaggcgg
     2401 tggcgatggc gcccgcttcc ggcgtcaggc cgagcagcgg gtagaacgcg atcaccgccc
     2461 aggtcgtgaa caatgccgcg tcgagcggat gcaggtagta ggcgccgaag gcgtcgacgc
     2521 tctccgcgct atggtgcatc tggtggccga ggcgccacag ccagttccac tggtgcgcga
     2581 gccggtgata ccagtagtgc atgagctcgt agaccaggat gccggcgatg gcgccgcccc
     2641 aggtgccgag cgccgcgccg tccaccaggc tccactgcgg cagcaccgtg ccccagaggc
     2701 gccccaccgc gaacgagacc ccgaacactg cgagcgagac cgcgccggcg cgccagcgcc
     2761 agccccgcgg cgcctcgtag ccgcgcgcgc gccagaagaa ctcgagcgcc aggaaaggcg
     2821 cgaggaacag caaggagtaa tagtcgatcc actcgagcag catcgcgaaa agcctccaaa
     2881 gttaggaatc gcaatgctgc ggcgcggctg ttccggacgt atttccggct ggttgccttt
     2941 tgttttcgtt ttgtgacgct tcggcgctgt caaatagtga tcggcgtcaa cgcgcgtcga
     3001 gcttcgagcg ttaggctgaa tgcatgtcgc ccaacgctcc tcacctgaag gccctgctca
     3061 acatggcacc gagcgccttt gcctgcggcg acatcggcaa gctgcccgcg atcaagtcgc
     3121 ttgcggcgct gaggaaagag gtccagggcc acttcggcaa gctctccgtc caggagatcg
     3181 acgccctggt cggctggttc aaccgcaact tcttccggct ggacgactag gaattcgggg
     3241 acggagccct aactccggcg acgcgcagct ttcggagtta gggctccgtc cccgaattcc
     3301 ctacctgcgg tgggcaggct tgaaggcccg cccgcggcgc ttgccgcgcg tcggcgagcc
     3361 cttcttggcg atggacccga agttgaaccg gttgtccttc atctcggcgg cacgctcgcg
     3421 catcatctgc tcggcgagcg tctcgcgggc gagcgccgcc ccgtactttc gtttcatgag
     3481 aatcagcctc tcgtggtggt cttgcggatt tccttctcgt cggaccattc cagcaggccg
     3541 ttgcggatgt tccagagctg aaggttgagc ttgtaataca cgtccttggc gtccttcacc
     3601 tgcttcacga tggaaatgat gttgccttcc aggcggtact gcgcgccgac catctggccc
     3661 atctcggcgg cggtgtcctt cgcgtacagc ccgctttgct ggcgcttgag ctcgtcgacc
     3721 tgcggattca tgccggcctg gtcgacggcg aagcgcaccc tgccgccctt ctgcagctcg
     3781 gaacggatgc tgtcggtgat ggcgcgggta tcgatgtact cgctggtctt gttcctcact
     3841 tcctgcaccg tgacgaccgg caggttggag ctcgcgatca ccggcgcctg cagcatcgag
     3901 cgcgccatgg actcggccat cagctgcagg tcggtcgagc cgaactggtt ggtcatggtc
     3961 tcgacgccgc gcgcgtcgcc gtagcgcgtc tgctccttgg cgcagccgcc catgagagcg
     4021 gcggcggcca gcgcaatcac gagatgagcc ttcattcctc actcctcaga atgaggcgga
     4081 aatcggccgc cttgttggtc ggagaaatgg tgttgatcac caggttggag ttctcgtaga
     4141 gcatcaacgg cttccacggc tcctcttccc aggcacgcat gccctgctgg tcgatccagc
     4201 ggaacttgta ggcgatgcgc ttcacgctct tgctgtcgtt gttcaggttg gcgtcgatct
     4261 tccacagctc cccgctcttc gagcagcgca tctccgtcgt ctggatcgtg gtgcgccgtc
     4321 cctcggcgag caccttcgcc ttgacgagat cgtccatcga gctcactttc gcctcgccga
     4381 tcgggcagcc gaacgcgaag ccgggctgct cgaagccttt cttcgtggcg catccggccg
     4441 cgagcatggc tgatgcggcc actgcgatca tccatttggg gttcatcttg cctccctcgg
     4501 tccattcacg aaaagctgat ggcgcaacaa gcgcaagtct accaccgcat agcgcccgga
     4561 caggctcacg gcagcgctgc gcgcgccttg cggcgtgtgc agcgtgatcg tgcgcgtgcc
     4621 tcgcggcaag cgcccgcggg caatggaaac gtccgaaggc agggctcgcc aagtgcgatc
     4681 gtcggccgag gcgagcgcga ccgagccgac ggcgacgatt cccgccgcca gcgccagcgc
     4741 gagcgtgcct tccctgtcgc cggcgcgctg cgcctggtat tgcagcgtgg cgctgacagt
     4801 cgagcggatc gtggcgcgca gcatgatgcc gggcatgtcg tccttcagcc ggcgccgggc
     4861 catgagatcg acgctggcga tccgcgtcgc cggcaggacg tgctcctcga gcgtgacctg
     4921 cgtcggcaac ggctcccacg acgtcgtcgt catgaccggg aacgcgttcg acaggaagag
     4981 gagcttgttg ttgaccacca ccggaagcgc gaactggcgc gattgcagcg ccggcgcgga
     5041 cccggtcccg atgatcacca gcacctcggt catgccgtcg tcgggcgcgg cgacgcgctg
     5101 atcgagcccg cgcagcgcct cttcaagcag cggctggccg ggctgcagct cgttcgcgag
     5161 gcggtagccc ggcgccgcga ggctcggctc gccgagcgct tcgtagatga acccggcgag
     5221 gtagtgcgac aaggcgctct ggtaggcgtt cctgagcgca ttcacttccg gggtatcgat
     5281 cgtctcgacc ggatagccgt tcagctcttt gaagctggtg tgcgcgccgc gctggcgcgc
     5341 cgactcttcc acctcggcgt attgccgggt gtggagctcg gcgatctgcg cctcgagctc
     5401 gtgcgcctgc ttgatcgcga cgcgcgcgct gtcgtagtcg cccatggcga ggtggttgag
     5461 cgccatatag gtgagcagca tcaccttttc gtagtcgtgg cccgagtagg cgcgcagctt
     5521 gtcgttgatg acgtagctcg acatgccgcg catcaggttc gacatctgcg ccagcccgcc
     5581 ttccgtcgtc gcctggatgc gcgcgtttgc cgcggcccag gccttctggc tctcagcgta
     5641 gcgcccgccg agccgctgca gcatccccag ctcgaggtag tagagcaggt ccttgtccgg
     5701 caggcggttg ttggactcga gaagctggat ggcgccgtcc acgctcccgc tggaggcgcg
     5761 atccagggtc gtatacagct ctgcgtcgta gctgcgcatc gcggcacagc cggacaacag
     5821 cagaaccatg acccagcgga tcacttgcgg gggaacgtaa tcagcttgtc cgaatcgctc
     5881 gcggtgccgg ccttggccgc gaggtacagc ggcgtcacct gcctgccgag ctcggcgagc
     5941 cgcgcggacc gattctgcgg cgtggggtgg gtggaaagga attccggcgg cccgccgccc
     6001 tccttgccca tcttgtccca cagcgtgacc gcggctttcg ggtcgtagcc cgcgcgcgcg
     6061 gcgagctcga tgccgatctg gtcggcctcg gattcgctgt cgcggctgtt cggcagctgg
     6121 atcgcgagca ccgcggcaag ctgcgccccg gtcagcgcga gcccggcgcc cgggccgctg
     6181 tcgcgcgagc cgatcgcgat cgcggcggcc gtagtcgcca ctgcgctggt catggcgatc
     6241 gacatgcgct cgcgcgtgtg gtctgcgagc gcgtgcgcga tctcgtggcc catcacctgc
     6301 gcgatctcgt cgtcggtcgc cttcagcttc tcccacatgc cggagtagat cgccatcttg
     6361 ccgcccgcca tgcagaaggc gttcaccgtc ttcggatcgt cgatcacctt cacttcccag
     6421 ttccagcttt tcgcatccgg gcgcacgcgc accgcctgcg cgaccaggcg atcggtgatc
     6481 tcgcgcactc tcttctcgcg ctcggtgccg gcttcgatct tcttcttctt gtcgagctcg
     6541 cccatcatct gcacgtaggc cgcggccgac tgcgacaccg ccatctgctc ggacaccacc
     6601 atgtactggc tgcggccggt cagcgggctg gtggtgcagc cggcgaggac gatcaggagg
     6661 gcgaaggccg aacggcgcat gggacgatgt gtctgattct tcccatgatt ttacgcgcgc
     6721 cgcggcttgc ggcggcgcga gatggattcc ggccggcggg cagcctagaa tcggtcgcgc
     6781 aagcccatgc tgcccctccg ccccggccag aagctccccc aggacccggt tttcaaggac
     6841 ccgccgcagt ggatccacga ggtcgcggcc gtggtgcttt cgccgcgcga ctcaagaagg
     6901 cgggcggtgc tgaagctcct cgaccggct
//
LOCUS       DATBSZ010000248        27269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_798484, whole genome shotgun sequence.
ACCESSION   DATBSZ010000248 DATBSZ010000000
VERSION     DATBSZ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 27269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27269
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798484"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VIG70_15745"
     CDS             complement(<1..827)
                     /locus_tag="VIG70_15745"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8252051.1"
                     /translation="MKLLALSLVLGLGAAQAQDLQPIKDAGPYVPSPQSVVSDMLRYA
                     DVGPSDFLIDLGSGDGRIVLTAAKVFGARGFGVEIKDDLVKKSNEAAQNEGLADRVKF
                     MKQDLFKTDMSQATVITMYLLPDTVNLLKDKFLAELKPGTRIVSHDYPLTGWIPEKYV
                     QMDLEDKVQISGVTTTLIYLYVVPAKVAGKWSAQMPPAVSKQPATLSLKQQLTRVTGS
                     VRLDGKDVPLEEVKLRGDRLSFKLAGRKGEFSGQVKGKSIEGVVESGGSKSPWSATLG
                     "
     gene            complement(824..2449)
                     /gene="recJ"
                     /locus_tag="VIG70_15750"
     CDS             complement(824..2449)
                     /gene="recJ"
                     /locus_tag="VIG70_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY8252052.1"
                     /translation="MHPVLARVYAGRNIRTAAELNYGLDGLIPPGSLKGIDDAAALLA
                     DAISERKKLLIVADYDADGATACAVGVRALRAFGANVDYLVPDRFKLGYGLTPELVEL
                     ASLRKPELLITVDNGIASVEGVARARSLGIATLITDHHLPGPELPAAACIVNPNQPGC
                     EFPSKALAGVGVMFYVMLALRAELRRRRFFKDGKEPNLAALTDLVALGTIADVVPLDA
                     NNRNLVAQGLKRLRAGRCTPGIAALLRAAGRPLAEASSFDLGFVAGPRLNAAGRLADM
                     SLGIECLTTDDEARAANCAQELDRLNRERRNIEAAMLQQALQAVEQVSSQESGTFFHP
                     DWHQGVVGIVASRLKERLHRPVICFARAEESGDPVLRGSGRSIPGLHLRDALDVVSKR
                     APGLIRRFGGHAMAAGLSIGAADYERFTGEFERAVDELLPAHARLRVVETDGALDAMY
                     FSLDVARLLDAGIWGQGFPQPVFCDTFAVENQRVVGERHLKLWLVKGGRRIEAMRYGA
                     LDQLPAQVRAAYRLSVNEFNGLKNIQLNVEHFE"
     gene            complement(2506..3468)
                     /locus_tag="VIG70_15755"
     CDS             complement(2506..3468)
                     /locus_tag="VIG70_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252053.1"
                     /translation="MHCELLIPRLFNTATDGKTAMRFAALELLLARGRTRAAAAQCVE
                     EWLHDAFDLGEGPFPAGALTLLAAGGAPGEARWARADPIHLRVMRDHLAIAPADAFEL
                     SAGEAETLCAALNGHFAGMDFTACEPRRWCAKVPEALSIDAGNPLDVAGMGVEPPRQA
                     ATLLNEAQMVLHAHPLNEAREARGEPAVNSVWLWGAGRATRASCAWQSVAADDPAVIG
                     AARLAGARHRLLPRSARDWLERLPEDGRHLALLDALRKQPENAVLESLERDWFEPLLA
                     ALRAGRVGMVTVHVPDGAEALSFETVRTDLRRFWRTKKPIEHYA"
     gene            complement(3468..4829)
                     /locus_tag="VIG70_15760"
     CDS             complement(3468..4829)
                     /locus_tag="VIG70_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY8252054.1"
                     /translation="MHAPLAIVDVETTGSDPASDRITEVAVLQADGVALTAQWSTLVN
                     PGASIPGVIQALTGITQEMVDAAPRFADIAEELYERLAGRVFVAHNARFDYGFLRREF
                     ERAGLKFLAKTLCTVRLSRRLYPGTAGHSLDSLIERHGLECEVRHRALPDAAVLWQFL
                     RTAVEEHGEDVVAVAARQIARQPALPPHLDRAAIEEIPEAPGVYLFYDEGAAPLYIGK
                     SRAMRSRVLQHFIATASWTPRVRRIEWRRTAGELGALLLEAELVKALDPVCNRQLRRP
                     GRLCGFAFDGKRLRLAGADEIDADTLPFLYGIWRSRGAALQALKAAADEHRLCLQTLG
                     FDEKKKGICLRHQLGRCAGVCAGKESIHLHHARVATALARLKSIDWPHRGALGVVEAD
                     ASRDATEVHVLDRWCYLGTARSDAEIAELLETRRPRFDYDHYRILSRHLGKRGVRVVE
                     LAA"
     gene            4898..5227
                     /locus_tag="VIG70_15765"
     CDS             4898..5227
                     /locus_tag="VIG70_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252055.1"
                     /translation="MRRFAAFALVLACACTPMQWQKPDFTAEQARTDEKECQQAAWRE
                     ASLRSWHYQSMVGPVFAQDASGRGYFVWPSTAMVDPYGYQLMEENRLAQFCMESKGYH
                     LVRVPKS"
     gene            complement(5217..6026)
                     /locus_tag="VIG70_15770"
     CDS             complement(5217..6026)
                     /locus_tag="VIG70_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEY8252056.1"
                     /translation="MRIYGVDFTCAPRKAKPITVASGRLKRDRLTVEEIEKLEDFAAF
                     EAFLARPGPWLGGFDFPFSLPRELVRDLSWPEDWPGLVAHCARMTRMELRAALDAYRS
                     TRQPGNKYVHRATDHPAGSSSPMKLVNPPVALMFHEGARRVLASGVYVPALSKGDSMR
                     VALEAYPGLLARKQLGIRDSYKSDTRREHTPARRAARKKIVQALKSGYPLNVRVTLDP
                     RLEKEVLADGSGDLLDAVICAVQAAWGWNRRESNFGLPAHAHPAEGWIVSS"
     gene            complement(6023..6874)
                     /locus_tag="VIG70_15775"
     CDS             complement(6023..6874)
                     /locus_tag="VIG70_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8252057.1"
                     /translation="MELTELRIFKAVAEHGGITRAAAALNRVQSNVTTRVKQLEERLG
                     AQLFHRQGRRLVLSSEGRLLLDYADRLLRLSAEAQAALKGHVPQGVLKIGALESTAAT
                     RLPAVLARYHRDYPAVRLELATATTGALVDKVLCEELEAAFVAEPFSAGGLQTRHVFT
                     EELVLITARNHPRVAAPGDLASRTVLAFPTGCSYRRRLELWLGRSHLAAERVMEYGSY
                     HAIVACAAAGAGIAVVPKSVLRATGMEAQVALHPLPPKIAQAKTMLVWRRGHQSVPLE
                     ALRKALA"
     gene            6999..7988
                     /locus_tag="VIG70_15780"
     CDS             6999..7988
                     /locus_tag="VIG70_15780"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY8252058.1"
                     /translation="MAGAQGSALAVAVCNAGGLGSLPCAMLSTDALRAELAAIQAATR
                     RPFNVNFFCHTPPAPNPQREAAWRAALAPYYEEYGIDPKAAAGGPVRAPFTPEAAEVL
                     AEFRPAVVSFHFGLPPQPLLERVRKLGSKILSSATTVDEARWLEAQGVDAIVAQGLEA
                     GGHRGNFLTDDLSTQIGTFALLPQIVAAVRLPVIAAGGIADAKGVAAAVALGAAGVQV
                     GTAYMLCPEATTSAVHRAALKSEAARHTALTNLFSGGPARGIVNRAMKELGPISGAAP
                     EFPLATAAIAPLRAKAESQGSGDFSPLWCGQNPGARREVPAAELTRSLARQIR"
     gene            complement(7957..8433)
                     /locus_tag="VIG70_15785"
     CDS             complement(7957..8433)
                     /locus_tag="VIG70_15785"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8252059.1"
                     /translation="MGDRAEIVDALYRFGAGQDLRDRRLFDSAFSAKAKLDFTGPARR
                     LGAAVSVFEGRQAIGDRIFSAIDQLDTTHTVTNPRVTHYDSRHASLVALVEAQHLPRG
                     DHGRHLLLKNIYTATLSKVQDRWLIDTLRIDNVWMTGDPSVLFPEATNESAVPVSA"
     gene            complement(8597..9094)
                     /locus_tag="VIG70_15790"
     CDS             complement(8597..9094)
                     /locus_tag="VIG70_15790"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8252060.1"
                     /translation="MNAEQLKALQAPIKERYKKDPAAAVITLRAQGRIGEGIACRVDT
                     GKALIEAGLHPATGGTGLQACSGDMLLEALVACAGVTLRAVATALAIPLRDAKLTAEG
                     DLDFRGTLGVAKDAPVGFREIRLKFDLDSDASEEQLKKLLELTERYCVVMQTIRGAPA
                     VVVSM"
     gene            complement(9091..9546)
                     /locus_tag="VIG70_15795"
     CDS             complement(9091..9546)
                     /locus_tag="VIG70_15795"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8252061.1"
                     /translation="MDAESALLLRNLVAKERVAHLATLRNEAPMASMTLYLPEADFSA
                     FYVHVSRLAWHTQDMAQDARVALSIAETDDRRADPFTLMRVTVRGGALQIQGENDELK
                     RRWLERFPAQAINFELADFSFWKITPRDARFVAGFGRIHNLSAAELKIP"
     gene            9577..10818
                     /locus_tag="VIG70_15800"
     CDS             9577..10818
                     /locus_tag="VIG70_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY8252062.1"
                     /translation="MRYELMVGLRYTRARRRNRFIGINSAVSMIGIAVGVWALIVVLS
                     VMNGFQKEVRTRILGVVSHLQILAGDNRLEDWRGVAKAVAQHPRVVGTAPFVQAQAML
                     SNGQAVRGAVVRGILPAEEEKVADLARHMRAGSLDELRPGEYGVVLGADLARSLGVLR
                     GDKIALVAPQGVVTPAGVIPRLKQFTVTGVFEAGIVDADSGLALVHMRDAQTLYQMGE
                     AVSGVRLKLDDLFAAREVAHELLARLPRDLFASDWTRSHANFFRAVEIEKRMMFLILG
                     LIVLVAAINIISTLVMAVTDKQADIAILRTLGAAPGSVLQIFMVQGMVLGVVGTLVGV
                     LTGIVTALNIDVIVPAIERAFNIKFLSKDVYLIPDLPSDLQSNDVLWIAAMALALSFL
                     ATIYPSWRAGRVNPAEALRYE"
     gene            10811..11509
                     /locus_tag="VIG70_15805"
     CDS             10811..11509
                     /locus_tag="VIG70_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8252063.1"
                     /translation="MNEAIHEVVSCNDLRKTYQGPAPVEVLRGVDLAVRAGERIAIVG
                     RSGSGKSTLLHVLGGLDAPTSGAVLVQGKPFSAMSETERGRVRNATLGFVYQFHHLLP
                     EFTAVENVAMPLFIRRMDRGEAKARAAKVLAEIGLGARLEHQPGELSGGERQRCAFAR
                     ALVTEPACVLADEPTGNLDSHTADEVFDAMVRLSASHGTAFVIVTHDSDLAARAQRIY
                     ELRDGVLHSSIIST"
     gene            complement(11461..11832)
                     /locus_tag="VIG70_15810"
     CDS             complement(11461..11832)
                     /locus_tag="VIG70_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252064.1"
                     /translation="MARARAPAQGSRVTHEEFVARYQAGRIRVKIDRQAAARFVAGRT
                     MLPLVLLPVLGLGVALALVGYVIAGVTIFVAALLLRYLVRRSSDGFLMSRALQDAEFY
                     RQVLAAQVLMIEECSTPSRSS"
     gene            complement(11793..12317)
                     /locus_tag="VIG70_15815"
     CDS             complement(11793..12317)
                     /locus_tag="VIG70_15815"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY8252065.1"
                     /translation="MPRKFFRKYHSNLEAVRSSRMVAAFGSWAQHPNLWHLNRRSVSG
                     AVAIGLLAGLVPGPLQMLAALLIAVPLKKNLPVAMLVTLYTNPFTIVPIYVLAYAYGR
                     LLLPGEHAVLPASFGMDNLGESLVALGKPLALGLVALGCTLAALGYVGVQLAWRAYVV
                     AAWRARARRRKAAA"
     gene            12354..14627
                     /locus_tag="VIG70_15820"
     CDS             12354..14627
                     /locus_tag="VIG70_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEY8252066.1"
                     /translation="MTLAALAFACGAAALQWQAELPSMAWAALLPLLLLRRRILALPL
                     AFAIGFFWAAAMAHLRMADRLAPELEGRDLDVVGVVASLPAISERAVRFELEVESSDQ
                     PLPRKLLLAWHRSPAAEEGAALLGEAVHPGERWLFTVRLRRPHGHVNPYGFDYEAWLL
                     ERGVGATGYVRQRGEQRRVGFRNSFFDRVEKAREAVRDRFQARLGATPAAGILAALAV
                     GDQRAISAEEWRLFNRTGVTHLMSISGLHVTLVSGLAAWLVAFGWRRVPTAVVRLPAR
                     KAAAAAAIAAALGYTLLAGFAVPAQRTFYMVSVVAAALWCGRIASPARTLALALAVVV
                     AADPWAPLAPGLWLSFAAVALIFYVASNEWRPAQWGRMQWAITVGLAPAALLLFGQVS
                     VAGPLANAVAIPVVSVIVTPLALLAVVLPLDLLLEAATWLVEWLLRFLEWCAALPGAL
                     WQQHVPSLWAAALAMAGAVWILAPRGVPWRAGGLALMAPALSLAPAAPAVGEAWITAL
                     DVGQGLAVVVRTANRTLLYDAGPAYGPEADSGGRIVVPMLRGAGVRGVDMMVLSHEDT
                     DHLGGALTVLETLEVRSLASSLARSHALNSLVATAGRCAAGSSWEWDGVRFEFLHPQP
                     GWETARRNNQSCVLHIAAGGGSILLTGDIERAAESALAGTIPKADVLVVPHHGSRTSS
                     SAEFIAAVGPRWAVVPAGYRNRFGHPAREVLERYASARVPVLRTDLDGAVSVVLQPDR
                     VRVQSERRLRPRYWRRV"
     gene            14673..14894
                     /locus_tag="VIG70_15825"
     CDS             14673..14894
                     /locus_tag="VIG70_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEY8252067.1"
                     /translation="MAKKGSGTVYRVTEVIGTSSNSWEDAARNAVKTAAGTLRDLRIA
                     EIEKLDVKIEDGKVVQFRARLALSFKYDS"
     gene            14901..15602
                     /locus_tag="VIG70_15830"
     CDS             14901..15602
                     /locus_tag="VIG70_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252068.1"
                     /translation="MFTAVVRITGAGRLDDFRERLRWLMVRDEDAEDYTEHHAAGTLE
                     YRFAPRRGIPFPAFTAASVDFPELRVEAEWERDGKRGRAVIENGQLVEQTSGPLDGAQ
                     VDVQAGDEGHLELGLACMAQPDGSLIGYAVTHARHTYWRYRGGVLDLLGTEEPDAALE
                     EVAFRFVEEWIWYDEEEAALERVRYANYGYPVRGANLKSEKLALLRANRGSHSSLDPE
                     GKAAREAIVRQWLKP"
     gene            15587..16462
                     /locus_tag="VIG70_15835"
     CDS             15587..16462
                     /locus_tag="VIG70_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8252069.1"
                     /translation="MAEAVEPRRRSVLCASPAGLHRIAYREWGDPRNRDVLVCVHGLT
                     RAGRDFDELARALCAQFRIVCPDIAGRGDSDRLADAKLYTWAQYVADMVTLIARLDVE
                     TVYWLGTSMGGFVGMALAAQAASPVKKLVLNDAGPVIGKVSLERIAKYVGQAPAFASL
                     EDAEKYVRAISAPFGPHSDAQWRFLTETWVRRHEDGAWRPHYDPRIAEAYRATMPEKD
                     LELWHLYDAVRCPTLLVRGEHSDVVSRQTAAEMTRRGPKAKVVEIHGVGHAPTLLQPA
                     QIALVRDFLLEGESS"
     gene            16474..17460
                     /locus_tag="VIG70_15840"
     CDS             16474..17460
                     /locus_tag="VIG70_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8252070.1"
                     /translation="MLVAVLATPAFAQQQSTLDTVRSKGFVQCGVNTGLAGFSQPDSK
                     GVWKGIDVDVCRGVAAAVFGDSSKVRYTPLTAQQRFTALQSGEVDILARNTTWTITRD
                     TSLGLNFVGVNYYDGQGFMVHRKLNVKSAKQLNGATVCVQPGTTTELNLSDYFRANKM
                     SFKPVVIEKMEEVLNAYFAGRCDVYTTDHSGLISTRASRAPKPEEHIILPEIISKEPL
                     GPAVRHGDDRWFDVVKWTMFAMLEAEEMGISSKNIDSQAGSQNPTVQRFVGASGDIGK
                     MLGIDNKWSFNIIKQVGNYGESFDANLKPLGFERGLNRLWNQGGLMYAPPIR"
     gene            17468..18451
                     /locus_tag="VIG70_15845"
     CDS             17468..18451
                     /locus_tag="VIG70_15845"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8252071.1"
                     /translation="MKTARRRLLALGLAPFAAPISTWAQPRKPWRIGYLAQADAAVGP
                     DTLGQLRAGMKDLGYEEKRDYLLEVRNAKGSPERLDQFAAELAALRVDLIVASSTPPA
                     VSALKATRSIPIVMAISGDPVGSGLIKSFARPGGNVTGTTLAFDEVNRKWLEMLIAIR
                     PGLSHVGVVSNPANVSMRGMLDPLEASSRALSLKLTVHHLSPGEDAETLLGKLKRNPP
                     EGLIVLPDAHIRTYVPQIAQGLARMRLPAVYGVAAYAEMGGLISYGPDQRAHYRRAAA
                     YVEKILKGANPAEIPVEFPTRFEFVVNLKSAREAGLSIPHELLLRADKVIR"
     gene            18448..19242
                     /locus_tag="VIG70_15850"
     CDS             18448..19242
                     /locus_tag="VIG70_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY8252072.1"
                     /translation="MSQKQKAESFRALHKGTFVIANAWDAGSARMLAGLGFQALATSS
                     GAHAGTLGRRDGQVTREEALEHARAIVEATDLPVSADLERCFADEPAAAAETIRLAAG
                     VGLVGCSIEDATKDKVYEFGHAVERVAAAVKVARSHHVVLTARTENFVRGRPDLDDTI
                     KRLQAFEKAGADVLMAPGLPDLEAVRKVCAALSKPYNFMAGIKGKSFSVAELQAAGVR
                     RISLATSLWRAAMSGLVEAAREVKDKGTFGYIERSLATPDISKYMK"
     gene            19239..19574
                     /locus_tag="VIG70_15855"
     CDS             19239..19574
                     /locus_tag="VIG70_15855"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8252073.1"
                     /translation="MKEVYIEEGCFILELSNTPDDPAVSIARARVPRGVTTRWHRVKD
                     TAERYVILEGKGRAEVGDSPPREVGPGDVVRIPPSCRQRIANIGDTDLVFLAICTPRF
                     VQEAYQQIE"
     gene            19669..20877
                     /gene="egtB"
                     /locus_tag="VIG70_15860"
     CDS             19669..20877
                     /gene="egtB"
                     /locus_tag="VIG70_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEY8252074.1"
                     /translation="MEEYAAIRRATVALTAPLSAEDCALQSMSDASPAKWHLAHTTWF
                     FETFVLEPHVRGYRAFDPAYRVLFNSYYHGIGDRHPRPERGMLSRPSLDEVLAYRNHV
                     DEALLAANDLPAALLELGLHHEQQHQELILTDVKHLLSRNPLKPAYQKHWPLTPIRAR
                     APRWIGFAGGLHEVGHAGPGFAFDNETPRHRVWLEDFQIASHPVTHGDFLDFIDDGGY
                     RRPELWLSAGWDLVRARGWQAPEYWLRRDDRWHTFTLHGEAPVDRNTPICHVSFFEAE
                     AFARWAGARLPTEAEWEIAAQGAPREGNFVESGALHPLALREDPSPGTLAQAYGDVWE
                     WTRSDYGPYPGYRPAPGAVGEYNGKFMCGQYVLRGGSCATPASHIRATYRNFFPPDAR
                     WQFSGLRLAK"
     gene            20929..21408
                     /locus_tag="VIG70_15865"
     CDS             20929..21408
                     /locus_tag="VIG70_15865"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8252075.1"
                     /translation="MKYWIALACFASGLAHANPAIDMDGYLRVSREAAQHRETRRVSE
                     AEFIRMSREPGTVILDARSREKFDELHVKHAINLPFPDIAIESLRTTIPDRNTRILIY
                     CNNNFANAEGPFPAKIARASLNLSTYIALYSYGYRNLYELGPLIDLKDSRLEFSSRP"
     gene            complement(21395..22348)
                     /locus_tag="VIG70_15870"
     CDS             complement(21395..22348)
                     /locus_tag="VIG70_15870"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8252076.1"
                     /translation="MKRIVLSLLLLAAATAFAQPYPSKPIRVAVAFPPGGPVDIIARL
                     MGPKLSELLGGQSVVVENVVGAGGNVAAMRVAKAAPDGYTVLAHSSAYAVNPTLIPNA
                     GYDGEKDFIPIAIVASQPNFIVVHADFPAKTLAELMARAKTDKLAFASAGNGTTPHLT
                     GEHLFKLRAKLDITHIPFKGGGPAAAAVLSGQPPVGVIAGSAPLPHIKAGKIRALAVS
                     SARRVASLPDVPTLGELGYPGMEDYTWVGFFVPAGTPPEIAQKLNDAVLKVANDPLIK
                     ERLEGLAFDVTAAPLSETAAYVKSELAKWGKVVRDVGAKVD"
     gene            complement(22358..23329)
                     /locus_tag="VIG70_15875"
     CDS             complement(22358..23329)
                     /locus_tag="VIG70_15875"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY8252077.1"
                     /translation="MTERKLTRDDLRKAAEKYKNWGRWGPHDEIGTLNHTRPEDIVAA
                     ARLVRKGKVISLALNFDQHGPQGAKSKYPALGRINPLHTMLRTGTDAYSGVLDKRGIR
                     AADDMVVMPLQCGTQWDGLGHVFYENFMWNGYDCREVTSAGAQKCGIEKTKNRMVGRG
                     VFLDVPRALGMEILPDGHAITGAELDRTAALQKVELKKGDFIIVRTGQMEAKLAAGSW
                     DGYPGGDAPGFSFETLEWIQRTQLAALASDTWGCEVRPNESEAGINQPWHWITIPIMG
                     MTMGEIFYLKELAEDCAADKVYEFLFVAPALPITGAVGSPTNPLAIK"
     gene            23328..24278
                     /locus_tag="VIG70_15880"
     CDS             23328..24278
                     /locus_tag="VIG70_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560484.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8252078.1"
                     /translation="MVWRSARGKRIAYQAAAIGAAVLVAWFLVSNTIQNLEERRIASG
                     FGFLGREAGFEIGESAFLRYSASDSYLRALFVGLSNTLAVALIGIVLATGLGTLIGLL
                     RLSKNFLLKTLSAAYVEFIRNVPLLVQLFFWYAVITEGMPGPREALSPLPGVFLSNRG
                     IYIPTFSSSPELQGFNFVGGAALSPEFAALLIGLVAYTAAFIAEIVRAGILAVDRGQG
                     EAAHALGLSRGQTTRLVILPQALRVIIPPMTSQYLNLTKNSSLAVAIGYPDLVSIANT
                     AMNQTGQAIEGIAIIMAVYLTISLSISAFMNWYNAHVAFK"
     gene            24275..25291
                     /locus_tag="VIG70_15885"
     CDS             24275..25291
                     /locus_tag="VIG70_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY8252079.1"
                     /translation="MRWARTNLFGSWPETVATLLILWILWRTVPPFIDWAFLDAVWRA
                     DPKACRAGEGACWGFIAEKHRFILFGTYPYELHWRPALATVILIGLWVFSAFRVFWRS
                     WLVLVWLFGLTVIGVLMWGGVLGMQYVENERWGGLILTLLLSTFGLAIAFPLSILLAL
                     GRRSEMPVIRGLCVGYIELIRGVPLISLLFMASVMLPLFLPSGVSIDKLLRAQIAMIL
                     FAAAYLAEVIRGGLQAIPRGQYDAAHALALPFWRRTYLIILPQALRIAVPALVNNFIA
                     FFKDTSLVLIIGLFDLLSTIKISLQDRTWTGFGVEAYLFASLVYFVFCAAMSRYSKSF
                     EHQR"
     gene            25288..26016
                     /locus_tag="VIG70_15890"
     CDS             25288..26016
                     /locus_tag="VIG70_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY8252080.1"
                     /translation="MIEMIKVSKSFGLFQALTDVDLAVARGEKVVVCGPSGSGKSTLI
                     RLVNRLEAHQSGRVIVDGVELDDDPKKVDLVRREVGMVFQSFNLFPHLTVLENLTLAP
                     IWVKHVPRKEAEETAMHFLARVRIPEQAGKYPSQLSGGQQQRVAIARALCMKPKLMLF
                     DEPTSALDPEMIKEVLDVMVELAREGMTMMVVTHEMGFAKTVADRVVFMDGGRVVEAA
                     PPAEFFGAPKTDRAKLFLSQVLGH"
     gene            26027..26983
                     /locus_tag="VIG70_15895"
     CDS             26027..26983
                     /locus_tag="VIG70_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8252081.1"
                     /translation="MKTLLIAVAVAVLGVNQAAAQSYPSRPVRIVVPATPGGAIDLIA
                     RSLAEKLTASLGQPVVVENKPGASNNLGTDFVAKSPPDGYTLVIVASSHATNKHLFKQ
                     MPFDPIKDFEPVVYTHVVPLLLAVHPSVQAKTVPELVGWIKANPDKAIYASSGPGSSL
                     HMAAELFMSMTGTKMHHVPYKGSSAAHPDLLAGRTAMIFDTITAVRGHVKSGAVRGIA
                     VTTAKRSSAMPELPTIAESLPGYDASTWGGILAPAGTSKEAVSKLNSSINAALKLEDV
                     RSRLMGAGIEIQGGTPEAFAEVIRIEVDKWGRIVKAAGIQPE"
     gene            complement(27005..>27269)
                     /locus_tag="VIG70_15900"
     CDS             complement(27005..>27269)
                     /locus_tag="VIG70_15900"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8252082.1"
                     /translation="GVGIGICQGPVIIGNVGSPHYMSYTVIGNPVNTAARLMEAAQMG
                     EVLVSSDFYEEIRRLVPQDRVESRGEVALRGKAETVRVYSIRP"
BASE COUNT         4308 a         9150 c         9330 g         4481 t
ORIGIN      
        1 ccgagggtcg cgctccaggg cgacttgctg ccgccggact cgaccacgcc ctcgatgctc
       61 tttcctttca cctggccgct gaactcgccc ttgcggccgg cgagcttgaa gctcagccgg
      121 tcgccgcgca gcttcacctc ctccagcggc acgtccttgc cgtcgaggcg cacgctaccc
      181 gtgacgcgcg tgagctgctg cttgagcgaa agcgtcgcag gctgcttgct caccgcggga
      241 ggcatctgcg cgctccactt gcccgcgacc ttcgccggca ccacgtacag gtagatgagc
      301 gtggtggtga ccccgctgat ctgcaccttg tcctcgaggt ccatctgcac gtacttctcc
      361 gggatccagc cggtgagcgg gtagtcgtgc gacacgatcc gcgtgcccgg cttgagctcg
      421 gcgaggaact tgtccttgag gagattcacc gtgtccggca ggagatacat cgtgatcacc
      481 gtcgcctgcg acatatcggt cttgaagagg tcctgcttca tgaacttcac gcggtcggcg
      541 agcccttcgt tctgcgccgc ctcgttcgac ttcttcacca gatcgtcctt gatctcgacg
      601 ccgaagccgc gcgcgccgaa caccttggcc gcggtcagca cgatgcggcc gtcgcccgag
      661 ccgaggtcga tcaggaagtc gcttggcccg acgtccgcgt agcgcagcat gtcggagacc
      721 acgctctgcg gcgagggcac gtacggtccc gcgtccttga tgggctgcag gtcctgcgcc
      781 tgggcagcgc cgagacccaa caccagtgaa agtgccagaa gcttcattcg aaatgctcca
      841 cgttgagctg gatgttcttg aggccgttga actcgttgac gctcagccgg taggccgccc
      901 gaacctgtgc gggcagctga tcgagcgcgc cgtagcgcat tgcctcgatg cggcggccgc
      961 ctttcaccag ccagagcttc aagtgccgct cgccgacaac gcgctggttt tccacggcga
     1021 atgtatcgca gaacaccggc tgcggaaagc cctggcccca gattccggcg tcgagcaggc
     1081 gcgccacgtc gagcgagaag tacatcgcgt cgagcgcgcc gtcggtctcg accacgcgca
     1141 ggcgcgcgtg cgccggcagc agctcgtcga ccgcgcgctc gaactcgccc gtgaagcgct
     1201 cgtagtcggc ggcgccgatg ctcaggcccg cggccatcgc gtgcccgccg aagcgccgga
     1261 tcagcccggg tgcgcgcttc gacaccacgt cgagcgcgtc gcgcaggtgc agtcccggga
     1321 tcgagcgacc cgagccgcgc agcaccgggt cgccgctttc ctccgcccgg gcgaagcaga
     1381 tcaccggccg gtgcagccgt tccttgaggc gcgaggcgac gattccgacc acgccctggt
     1441 gccagtcggg gtggaagaaa gtaccgcttt cctgggaaga aacttgttca acggcctgca
     1501 aagcctgttg cagcatggcg gcttcgatgt tgcgccgctc gcggttgagg cggtcgagct
     1561 cctgcgcgca gttcgccgcg cgcgcctcgt cgtcggtggt caggcattcg atgccgaggc
     1621 tcatgtcggc gagccgcccg gcggcgttga gccgcggccc ggcgacgaac ccgaggtcga
     1681 agctcgaggc ttcggcgagc ggccggccgg cggcgcgcag cagcgcggcg atccccggcg
     1741 tgcagcgccc ggcgcgcagc cgcttcaggc cctgcgccac caggttgcgg ttgttcgcgt
     1801 cgagcggcac cacgtcggcg atcgtgccga gggcgacgag atcggtgagc gcagcgagat
     1861 tgggctcttt cccgtccttg aagaatcttc ttcttctcag ctcggcgcgc aaggcgagca
     1921 tgacgtagaa catcacgccc acgccggcga gcgccttgga ggggaattcg cagccgggct
     1981 ggttcggatt gacgatgcag gccgccgcgg gaagctcggg cccgggaagg tggtggtcgg
     2041 tgatcagagt ggcgatgccg aggctgcgcg cgcgcgccac gccttcgacg ctggcgatgc
     2101 cgttgtcgac ggtaatcagc agttcaggtt ttcttaatga agcaagttcc accagttcgg
     2161 gcgtgagccc gtagccgagc ttgaagcggt ccggaacgag gtagtcgacg ttggcaccga
     2221 aggctctcag cgcgcgcacg cccaccgcac aggccgtggc gccgtcggcg tcgtagtcgg
     2281 cgacgatgag aagcttcttt ctttccgaaa tcgcatccgc cagaagcgcc gcggcgtcgt
     2341 cgatgccctt tagcgaaccc gggggaatca gcccgtccag gccgtagttc agctcggcgg
     2401 cggtgcggat gttgcggccg gcatagacgc gggcaagcac gggatgcatg ccggattcga
     2461 gcagcgcgcg gcggtgcgtt tcgttgtagg tgcgctcgac gatcttcacg cgtagtgctc
     2521 gattggtttt ttcgtccgcc agaagcggcg caggtccgtg cggacggttt cgaagcttag
     2581 cgcttccgcg ccgtcgggca catggacggt caccatcccg acgcggccgg cgcgtagcgc
     2641 ggcgagcagc ggctcgaacc agtcccgctc cagactctcg aggacagcgt tttcgggctg
     2701 cttgcgcagc gcgtcgagca gcgcgagatg ccggccatcc tcgggcagcc gctcgagcca
     2761 gtcgcgcgcc gagcgcggca gcaggcggtg acgcgcgccg gcgagccgcg cggcgccgat
     2821 caccgcgggg tcgtcggcgg cgaccgattg ccaggcgcag cttgcgcgcg tcgcgcggcc
     2881 ggcgccccag agccagacgc tgttcaccgc gggctcgcca cgcgcctcgc gcgcctcgtt
     2941 cagcgggtgc gcatgcagca ccatttgcgc ctcgttgagc agcgttgccg cctgccgcgg
     3001 cggctcgacc cccatacccg ccacgtcgag cggattgccg gcatcgatgg acagcgcctc
     3061 cgggactttc gcgcaccaac ggcgcggctc gcaggcggtg aagtccatgc ctgcgaagtg
     3121 tccgttcaat gccgcgcaca gcgtctcggc ttccccggca gacagctcga aggcgtcggc
     3181 gggggctatc gccaggtggt cgcgcatgac gcgcaagtgg atgggatcgg ctcgtgccca
     3241 gcgcgcctcg ccaggggcgc cgcccgccgc cagcagcgtc agcgcgccgg cgggaaacgg
     3301 cccttctccc aggtcgaacg cgtcgtgcag ccactcttcc acgcactgcg ccgccgcggc
     3361 gcgggttcgg ccgcgcgcga gcaagagctc gagcgcagcg aagcgcatgg ccgtttttcc
     3421 gtccgtggcg gtgttgaaga ggcgcgggat caggagctcg cagtgcatca ggcggcgagc
     3481 tcgacgaccc ggacgccgcg cttgccgaga tggcgcgaaa ggatccggta gtggtcatag
     3541 tcgaaacgcg ggcgccgcgt ctcgagcagc tcggcgatct cggcatcgga ccttgcggtg
     3601 ccgaggtagc accagcggtc gaggacatgc acctcggtgg cgtcgcggct tgcgtcggcc
     3661 tcgaccacgc cgagcgcgcc gcggtgcggc cagtcgatgc tcttcaggcg ggcgagggcg
     3721 gtagcgacgc gcgcatggtg caggtggatg ctttccttgc cggcgcaaac gcctgcgcag
     3781 cgcccgagct gatgcctcag gcagattcct tttttctttt catcgaagcc aagggtctgc
     3841 agacaaagcc gatgctcgtc cgccgccgcc ttcaacgcct gcaatgccgc gccgcgcgag
     3901 cgccagatgc cgtagaggaa aggcagcgta tcggcgtcga tttcgtcggc gccggcgagc
     3961 cgcagccgct tgccgtcgaa cgcgaagccg cacagccgcc cgggccggcg cagctggcgg
     4021 ttgcagaccg gatcgagcgc cttcacgagc tcggcctcga ggagcagcgc cccgagctcg
     4081 ccggcggtgc gccgccactc gatgcgccgc acgcgcggcg tccagctcgc ggtggcgatg
     4141 aagtgctgca acacccggga gcgcatcgcg cggctcttgc cgatatagag cggcgccgcg
     4201 ccctcgtcgt agaacagata cacgccgggg gcttccggaa tctcctcgat cgccgcgcgg
     4261 tcgaggtgcg gcggcagggc cggttgccgc gcgatctggc gcgctgcgac cgccaccacg
     4321 tcctccccgt gctcttctac ggccgtgcgc aggaattgcc agagcacggc cgcgtcgggg
     4381 agggcgcggt ggcgcacctc gcattccagg ccgtggcgct cgatcaggct gtcgagacta
     4441 tggccggccg tgccgggata cagccggcgc gacagccgca cggtgcaaag cgtcttcgcc
     4501 aggaacttga ggccggcgcg ctcgaattcg cggcgcagga agccgtagtc gaagcgcgcg
     4561 ttgtgcgcga cgaaaacgcg tccggcgagc cgctcgtaca gctcctcggc gatgtcggcg
     4621 aagcgcggcg cggcgtccac catctcctgc gtgatgccgg tgagcgcctg gatgacgccg
     4681 gggatcgacg ctccgggatt gaccagcgtc gaccactgcg cggtcagggc gacgccgtcg
     4741 gcttgcagca ccgcgacctc cgtgatgcgg tcgctggccg ggtcgctgcc cgtagtctcc
     4801 acatcgacga tcgcaagcgg tgcatgcatg gatatacagt atcggcactc tagcgcactt
     4861 tgaacccgcg aagcgccgct ctggtctata aggcggtatg cgccgattcg ccgctttcgc
     4921 gctcgtcctt gcctgcgcct gcacgccgat gcaatggcag aaaccggatt tcacggccga
     4981 gcaggcgcgc accgacgaga aggaatgcca gcaggccgcg tggcgcgagg ccagccttcg
     5041 ctcctggcac taccagtcga tggtcgggcc ggtgtttgcg caggatgcga gcgggcgcgg
     5101 ttacttcgtc tggccaagca cggcgatggt cgacccctac ggctaccagc tgatggagga
     5161 gaaccggctg gcccagtttt gcatggagtc caagggctac cacctcgtaa gagtgcctaa
     5221 gagctgacga tccacccctc ggccgggtgc gcgtgcgcag ggaggccgaa gttcgattcg
     5281 cgcctgttcc agccccacgc ggcttgcacc gcgcaaatca ccgcgtcgag caggtcgccc
     5341 gagccgtccg ccaagacttc cttctccagc cgtggatcca gcgtgacgcg aacgttcaag
     5401 gggtagcccg atttcagcgc ctgcacgatc ttcttgcgcg ccgccctgcg ggccggcgtg
     5461 tgctcgcgcc gcgtgtcgct cttgtaggaa tcgcggatgc cgagctgctt gcgcgccagc
     5521 aggcccggat aggcttcgag cgccacacgc atgctgtcgc ctttggatag tgctggtacg
     5581 tagactccag aggcgagtac tcgccgcgcg ccttcgtgga acatcagcgc caccggcgga
     5641 ttgacgagct tcatcgggct ggacgagcct gcgggatggt cggtggcgcg gtggacgtac
     5701 ttgtttcccg gctggcgtgt gctgcgatac gcatccagcg cagcccgcag ctccatccgc
     5761 gtcatgcgcg cgcaatgcgc gaccaggccg ggccagtcct caggccagct caggtcgcgc
     5821 accagctcgc gcggcaggct gaacggaaag tcgaagccgc caagccacgg gccgggacgc
     5881 gcgagaaacg cctcgaacgc ggcgaaatct tcgagcttct cgatttcctc gactgtcaga
     5941 cggtctcttt tcaatcgacc tgaagcgacg gtgatcggct tcgccttgcg cggggcgcag
     6001 gtgaaatcga cgccgtagat cctcaagcga gcgccttgcg cagcgcctcg agcgggaccg
     6061 actggtggcc gcgccgccag acgagcatgg tcttcgcctg agcgatcttc ggcggcagtg
     6121 gatgcagcgc gacctgggct tccattccgg ttgcgcgcag caccgacttc ggcaccacgg
     6181 cgatgccggc gcctgcggcg gcgcacgcga cgatcgcgtg gtaggagccg tactccatga
     6241 cgcgctccgc ggcgaggtgc gagcggccga gccacagctc gaggcggcgc cgatatgagc
     6301 agccggtggg gaaggcgagc acggtacggc tggcgaggtc gcccggcgca gcgacacggg
     6361 gatggttccg ggcggtgatg agcaccagct cctcggtgaa gacgtgccgc gtctgcaggc
     6421 cgccggcaga gaacggctcg gcgacgaacg cggcctcgag ctcctcgcac agcaccttgt
     6481 cgaccagcgc gccggtggtc gcggtagcaa gctcgagccg caccgccggg tagtcgcgat
     6541 ggtagcgggc gagcaccgcg ggcagccgcg tcgccgcggt gctttcgagc gcgccgatct
     6601 tcagcacgcc ctgcggcaca tggcccttaa gcgcggcctg cgcctccgca gacaggcgca
     6661 gcagccggtc ggcatagtcg agcagcagcc tgccctcgga ggagagcacc agcctgcgcc
     6721 cctggcggtg gaacagctgc gcgccgagcc gctcctcgag ctgcttgacg cgcgtggtga
     6781 cgttggattg cacccggttc agcgccgccg cagcccgggt gatgccgccg tgctcggcga
     6841 ccgccttgaa aatccgcagc tcggtgagct ccatgttctc gaatagagaa tgaaatgttc
     6901 ttgattattc atttttcaga aacccgccgt caacctacac tgcgccggtg accctcaaag
     6961 cgctgctcgg cgtcgagctg ccggtgatcc aggcgccgat ggccggcgcg cagggcagcg
     7021 cgctcgccgt cgcggtgtgc aacgccggcg gcctgggctc gctgccctgc gccatgctct
     7081 ccaccgatgc cctgcgcgcc gagctcgcgg cgatccaggc ggccacccgc cggccgttca
     7141 acgtcaattt cttctgccac accccgcccg cgccgaatcc gcagcgcgaa gcggcctggc
     7201 gcgcggcgct cgcaccgtac tacgaggaat atggcatcga tccgaaagcg gcagccggcg
     7261 ggccggtgcg cgcgcccttt acgcccgaag ccgccgaggt gctggccgag ttcaggccgg
     7321 cggtggtgag cttccatttc ggcttgccgc cgcagccgct actcgaacgc gtgcgcaagc
     7381 tcggatcgaa gatcctttcg tcggccacca cggtcgacga ggcgcgctgg ctggaagcgc
     7441 agggggtcga tgcgatcgtc gcgcagggtc tcgaggccgg cgggcatcgc ggcaacttcc
     7501 tcaccgacga cctgagcacc cagatcggca cttttgcgct gctgccgcaa atcgtggccg
     7561 cagtcaggct tccggtgatc gccgccggcg gaatcgccga tgcgaagggc gtcgccgccg
     7621 cggtggcgct cggcgccgcc ggcgttcagg tcggtaccgc ctatatgctg tgcccggaag
     7681 cgacgaccag cgcggtacat cgcgccgcgc tgaaaagcga agccgctcgc cacacggcgc
     7741 tcaccaacct gttcagcggc ggcccggcgc gcggcatcgt caatcgcgcc atgaaggaac
     7801 tggggccgat cagcggcgcg gcgcccgaat ttccgctggc gacggcggcc atcgccccgc
     7861 tgcgcgcgaa ggccgaaagc caggggagcg gcgatttttc gccgctgtgg tgcggtcaga
     7921 atcccggcgc gcgccgcgag gtaccggccg ccgagcttac gcgctcactg gcacggcaga
     7981 ttcgttagtc gcttccggaa acagcactga cggatcgccc gtcatccaga cgttgtcgat
     8041 gcgcagggtg tcgattaacc agcgatcctg caccttggaa agcgtcgcgg tgtagatgtt
     8101 cttcagcagc aggtgccgcc cgtggtcgcc gcgaggcagg tgctgcgcct cgaccagcgc
     8161 gaccaggctc gcgtgccggc tgtcgtaatg cgtcacgcgg ggattggtca cggtgtgggt
     8221 cgtgtcgagc tgatcgatgg cggaaaagat gcggtcgccg attgcctgcc gcccctcgaa
     8281 caccgatacc gcagcgccaa ggcgccgggc ggggccggtg aaatcgagct ttgccttcgc
     8341 agagaaagcg gagtcgaaca agcggcggtc gcgaagatcc tggccggcgc cgaagcggta
     8401 aagggcgtcg acgatctcgg cgcgatcgcc caccggaacc ccgaaagcgc tcaaggcgct
     8461 gggctgaggc gtctggaaaa agtcatcacg tgcattcatg gctgctcctg atcaaggtct
     8521 gtggagacta gaccgtggaa gcagcgccga tgtgacaggc gggatcttca gtgttgcgaa
     8581 cgtaacaacg atgcgttcac attgaaacca ccacggctgg cgcgccacgg atcgtctgca
     8641 tcaccacgca gtagcgctcg gtgagctcga ggagcttctt cagctgctcc tcggaggcgt
     8701 cgctgtccag gtcgaacttc aggcggatct cgcgaaagcc caccggcgcg tctttcgcga
     8761 cccccaaggt gccgcggaag tcgaggtcgc cctcggcggt cagcttggcg tccctcaaag
     8821 gaatggcgag ggcagtagcg accgccctga gggttacccc ggcgcacgcg accagggctt
     8881 cgagcagcat gtcgcccgag cacgcctgca gccctgtgcc gccggtggcc gggtgcagcc
     8941 ccgcctcgat cagcgccttc ccggtgtcga cgcggcaggc gatgccttcg ccgatgcggc
     9001 cttgcgcgcg cagcgtgatg accgcggcgg cgggatcctt cttgtagcgt tccttgatcg
     9061 gcgcctgcag cgccttgagt tgttcagcat tcatggaatt ttgagctccg cagcggagag
     9121 attgtggatg cgaccgaagc cggcgacgaa gcgcgcgtcg cgcggcgtga tcttccagaa
     9181 cgagaaatcg gcgagctcga agttgatcgc ctgcgccggg aagcgctcga gccagcggcg
     9241 cttcaattca tcgttctcgc cttgaatttg gagtgcgccg ccgcgcacgg tcacgcgcat
     9301 gagggtaaat ggatcggcgc ggcggtcgtc ggtctcggcg atcgacagcg cgacgcgcgc
     9361 atcctgcgcc atgtcctggg tatgccaggc gaggcggctg acgtgcacgt agaacgcgga
     9421 gaaatccgct tccggcaggt agagggtcat cgacgccatc ggcgcctcgt tgcgcagcgt
     9481 ggcgaggtgg gcgacgcgct ccttcgcgac gagattcctg aggagaagcg cgctctcggc
     9541 atccaccgcg tcgattgtag taagttacgg ccctccatgc gatacgagct catggtcggc
     9601 ttgcgctaca cccgcgcccg ccggcgcaat cgcttcatcg gcatcaattc cgcggtttcc
     9661 atgatcggta tcgcggtcgg cgtgtgggcg ctgatcgtgg tgctgtccgt gatgaacggc
     9721 ttccagaagg aagtcagaac gcggattctc ggcgtggtgt cgcacctgca gatcctggcc
     9781 ggcgacaacc gcctcgagga ctggcgtggc gtcgcgaaag cggtggcgca gcacccgcgt
     9841 gtcgtcggca ccgcgccctt cgtccaggcg caggcgatgc tttccaacgg ccaggcggtg
     9901 cgcggcgccg tggtgcgcgg catcctgccg gccgaggagg agaaggtcgc ggatctcgcg
     9961 cgccacatgc gcgccggctc gctcgacgag ctgcggccgg gcgaatacgg cgtggtgctc
    10021 ggcgccgatc ttgcgcgctc gctcggcgtg ctgcgcggcg acaagatcgc gctggtagcg
    10081 ccgcaggggg tcgtgacccc ggcgggggtg atcccgcgcc tcaagcagtt caccgtcacc
    10141 ggcgtgttcg aggccggcat cgtcgacgcc gattccggac ttgcgctcgt ccatatgcgc
    10201 gacgcgcaaa ccctctacca gatgggcgag gcggtcagcg gcgtgcgcct caagctcgac
    10261 gacctgttcg ccgcgcgcga ggtcgcccac gagctgctcg ccaggctgcc gcgcgatctc
    10321 ttcgcctccg actggacgcg cagccacgcc aacttcttcc gcgcggtcga gatcgagaag
    10381 cgcatgatgt tcctgatcct cgggctgatc gtgctggtcg cggcgatcaa catcatctcg
    10441 accctggtga tggcggtcac cgacaagcaa gccgacatcg ccatcctgcg cacgctcggc
    10501 gcggcgccgg gctcggtgct gcagatcttc atggtccagg gcatggtgct cggcgtcgtc
    10561 ggcacgctgg tgggcgtgct gacgggcatc gtcacggcgc tgaacatcga cgtcatcgtt
    10621 ccggcgatcg agcgcgcttt caacatcaag ttcctttcca aggacgtcta cctcatcccc
    10681 gacctgccgt ccgacctgca gtcgaacgac gtcctctgga tcgcggcgat ggcgctcgcc
    10741 ctgtcttttc tcgccacgat ctacccgagc tggcgcgcgg gccgcgtcaa tccggccgaa
    10801 gcgcttcggt atgaatgagg ctattcatga agtcgtctcc tgcaatgacc tgagaaagac
    10861 gtatcaggga ccggcgccgg tggaggtgct gcgcggcgtg gacctggcag tgcgcgccgg
    10921 agagcgtatc gccatcgtcg gccgctcggg ctccggcaag tcgacgctgc tgcacgtcct
    10981 cggcgggctg gatgcgccga cctcgggcgc ggttctcgtc cagggcaagc cgttcagcgc
    11041 catgagcgag accgagcgcg gccgcgtgcg caatgcgacg ctcggcttcg tctaccagtt
    11101 tcaccacctt ctgcccgagt tcacggccgt ggagaacgtg gcgatgccgc tcttcatccg
    11161 acgaatggac cgcggcgagg ccaaggcgcg ggctgcaaaa gtgctcgccg agatcggcct
    11221 tggcgcgcgc ctcgaacacc agccgggcga gctctcgggc ggcgagcgcc agcgctgcgc
    11281 gttcgcgcgc gcgctggtca ccgagcccgc ctgcgtgctc gcggacgagc cgaccggcaa
    11341 cctcgactcg cacaccgccg acgaggtatt cgacgcgatg gtgcgcctct ccgcgtcgca
    11401 cggcacggcg ttcgtgatcg tcacccacga cagcgacctc gccgcgcgcg cgcagcgcat
    11461 ctacgagctg cgtgacgggg tgctgcactc ctcgatcatc agcacctgag cggcgaggac
    11521 ctggcggtag aactcggcgt cctgaagcgc ccgcgacatc aggaacccgt cgctgctgcg
    11581 gcgcacgaga tagcgcagta gtagagcggc cacgaagatc gttactccag caatgacgta
    11641 gccgaccagg gcgagtgcaa cgcccaagcc gagcaccggc agcaggacga gcggcagcat
    11701 ggtgcggccg gcgacgaagc gcgcggcggc ctggcggtcg atcttcacgc ggatgcgccc
    11761 cgcctggtac cgggcgacaa actcctcgtg cgtcacgcgg ctgccttgcg ccggcgcgcg
    11821 cgcgcgcgcc acgcggcaac gacataggcg cgccacgcga gctggacccc cacatagccg
    11881 agagccgcca gcgtgcagcc cagcgcaacc agcccgaggg ccaggggctt gccgagcgca
    11941 accagggact cgcccaggtt gtccatgccg aacgatgcag gcaaaacggc gtgctccccg
    12001 ggcaggagca accgcccgta cgcgtaggcg aggacataga tcggcacgat cgtgaacgga
    12061 ttggtgtaga gcgtgaccag cattgccacc ggaaggtttt tctttagcgg cacggcaatc
    12121 aggagggcgg cgagcatctg cagcggtccg ggcaccagtc ctgcgagcag gccgatcgcc
    12181 accgcgccgg acaccgagcg ccggttgagg tgccacaggt tcgggtgctg cgcccagctc
    12241 ccgaatgccg cgaccatgcg gctcgagcgc accgcctcga gattggaatg gtacttgcgg
    12301 aaaaacttgc ggggcacggt cagccaggag gaagcaggcg cgttgcattg tagatgaccc
    12361 tggcagcgct ggccttcgca tgcggtgcgg ccgcgctgca gtggcaggcc gagctgccgt
    12421 cgatggcatg ggcggcgctg ctaccgcttc tcttgctgag aagaagaatc cttgccctgc
    12481 cgctggcgtt cgctatcggc ttcttctggg ccgccgccat ggcgcacctg cgcatggccg
    12541 accggctggc gccggagctc gaaggccgcg acctcgacgt cgttggcgtg gtcgcgagcc
    12601 tgcccgcgat aagcgaacgc gcggtgcgct tcgagctcga agtggagtcc tccgatcagc
    12661 ccctgccgag gaagctgctg ctggcgtggc accgcagccc cgcggccgaa gagggcgcag
    12721 cgctcctcgg agaggctgtg catccgggcg agcgctggct cttcacggtg cggctgcgcc
    12781 ggccgcatgg ccacgtcaac ccgtacggct tcgactatga ggcctggctt ctcgagcgag
    12841 gcgtcggggc cacgggatat gtgaggcaac ggggcgagca gcgccgagtc gggttcagga
    12901 acagcttttt cgatcgggtg gaaaaagcca gggaagcggt acgcgatcgc ttccaggcgc
    12961 ggctcggtgc gaccccggcg gccggaattc tcgccgcgct ggcggtagga gaccagcgcg
    13021 cgatttccgc ggaggagtgg cggctcttca accggacggg cgtcacgcac ctgatgtcca
    13081 tttcgggtct gcacgtgacg ctcgtctcgg ggctcgcggc gtggctggtc gcgttcggct
    13141 ggcggcgagt gccgacagcg gtagtgcggt tgccggcgag gaaggccgcg gctgcggcgg
    13201 cgatcgcggc ggcgctcggc tatacgctcc ttgccggctt cgcagtgccg gcgcagcgta
    13261 ccttctacat ggtgagcgtg gtcgcggccg cgctgtggtg cgggcggatc gcttcgcccg
    13321 cgcgcacgct tgccctcgcg ctggcggtgg tcgtcgcagc cgacccctgg gcgccgctcg
    13381 cgccggggct gtggctctcg ttcgctgcgg tcgctctgat cttctacgtc gcgtcgaacg
    13441 aatggcgccc ggctcaatgg ggccggatgc agtgggccat caccgtggga ttggcgcccg
    13501 ccgcgctgct gctgttcggc caggtctcgg tcgccgggcc gctcgccaac gccgtcgcca
    13561 ttcctgtggt gtcggtgatc gtgacgccgc tcgcgcttct tgccgttgta ctacctctag
    13621 accttttgtt ggaagcagct acctggttgg tcgagtggct gctgcgcttc ctcgaatggt
    13681 gcgcagcgct tcccggagcg ctgtggcagc agcacgtgcc gtccctgtgg gccgcggcgc
    13741 tcgccatggc gggcgcggtc tggatcctgg cgccgcgcgg cgtgccgtgg cgcgccggcg
    13801 gactcgcgct gatggcgccg gcgctttcgc tcgccccggc tgcgccggcg gtgggcgagg
    13861 cctggatcac cgcgctcgac gtcggccagg gcctggcggt cgtggtgcgt accgcgaatc
    13921 gaacgctgct ctacgacgcc ggcccggcct atggccccga agcggacagc ggcgggcgca
    13981 tcgtcgttcc gatgctgcgc ggcgccgggg ttcgcggcgt ggacatgatg gttctcagcc
    14041 acgaggacac ggatcatctc ggcggcgcgc tcaccgtgct ggaaacgctg gaggtccgct
    14101 cgctcgcgtc ctcgcttgcg cgctctcatg ccctgaactc gctggtcgcc accgcgggcc
    14161 gatgcgccgc cggcagcagc tgggaatggg acggggtgcg cttcgaattc ctgcatccgc
    14221 agccgggctg ggagactgcg cgccgcaaca accagagctg cgtgctgcac atcgccgccg
    14281 gcggaggctc cattctgctg acgggggata tcgagcgcgc cgcggaatcg gcgctggccg
    14341 ggacgattcc gaaggcggat gtgctggtcg tgccccacca cggcagccgc acctcgtcga
    14401 gcgccgagtt catcgccgcc gttgggccgc gctgggcggt ggtgcccgcg gggtaccgca
    14461 accgcttcgg acacccggcg cgcgaggtgc tggagcgcta tgcgagcgcg cgggtcccgg
    14521 tcctgcgcac cgatctggac ggcgcggtca gcgtggttct gcagccggat cgcgttcggg
    14581 tccagtccga acgacgcttg cgcccccggt attggcgtcg cgtatagaat catctcgcgg
    14641 gggtctcccg cgaccagagg agaggatcga ccatggcgaa gaaaggcagc ggaacggtct
    14701 atcgcgtgac cgaggtgatc ggcaccagca gcaactcgtg ggaagacgcc gcgcgcaatg
    14761 cggtcaagac cgcggcgggc acgctgcgcg atctgcgcat cgcggagatc gagaagctcg
    14821 acgtcaagat cgaggacggc aaggtggtgc agttccgggc gcggctcgcg ctgtccttca
    14881 agtacgactc gtaaattggg atgttcaccg cggtcgtgcg catcaccggc gcagggcggc
    14941 tcgacgattt ccgcgagcgc ctgcgctggc tgatggtgcg cgacgaggac gccgaggact
    15001 acaccgagca ccacgcagcg gggacgctcg agtaccgctt cgctccgcgg cgcggcatcc
    15061 cctttcccgc gtttaccgcc gcatcggtgg actttcccga gctgcgcgtc gaggccgaat
    15121 gggagcgcga tggcaagcgc ggccgcgcgg tgatcgagaa cggccagctc gtcgagcaga
    15181 cctccggccc cctcgacggc gcgcaggtgg acgttcaggc cggcgacgaa ggccacctcg
    15241 agctcggcct cgcctgcatg gcgcaacccg acggcagcct catcggctac gcggtcaccc
    15301 acgcgcgcca cacgtactgg cgctaccgcg gcggggtgct ggatctgctc ggcaccgagg
    15361 agcccgacgc tgcgctcgag gaggtcgcgt tccgcttcgt cgaggagtgg atctggtacg
    15421 acgaggagga ggccgcgctc gagcgcgtgc gctatgccaa ctacggctac ccggttcgcg
    15481 gcgcgaacct gaagtcggag aagctcgcgc tgctgcgcgc gaaccgcggc agccattcct
    15541 ccctcgaccc ggaagggaaa gccgcccgcg aggcgatcgt caggcaatgg ctgaagccgt
    15601 agagccgcgc cggcgctcgg tgctttgtgc ctcgccggcc ggcctgcacc gcatcgccta
    15661 ccgcgaatgg ggtgacccgc gcaaccgcga cgtgctggtg tgcgtgcacg gcctcacgcg
    15721 cgccgggcgc gatttcgacg agctggcgcg cgcgctgtgc gcgcagttcc gcatcgtgtg
    15781 cccagacatc gccgggcgcg gcgactccga ccggctggcc gacgcgaagc tctacacctg
    15841 ggcgcagtac gtggccgaca tggtcacgct catcgcccgg ctcgacgtcg aaaccgtgta
    15901 ctggctcggc acctcgatgg gcgggttcgt cggcatggcg ctcgccgcgc aggcggcgag
    15961 cccggtgaag aagctggtgc tcaacgatgc cggccccgtg atcggaaagg tgtcgctcga
    16021 acgcatcgcg aaatacgtcg gccaggcacc ggcctttgcg tccctcgagg acgcggagaa
    16081 gtacgtccgc gccatctcgg cgccgttcgg cccgcacagc gacgcgcaat ggcgcttcct
    16141 gaccgagacc tgggtgcgcc gtcacgagga cggcgcctgg cgcccccatt acgatccgcg
    16201 catcgccgag gcctaccgcg cgacgatgcc cgaaaaggat ctcgagctct ggcacctcta
    16261 cgacgctgtg cgctgcccga cgctgctcgt ccgcggcgag cactcggacg tcgtttcccg
    16321 tcagaccgcc gcggagatga cgcgccgcgg cccgaaggcg aaagtcgtcg agatccacgg
    16381 cgtgggccac gcgccgaccc tattgcaacc cgctcagatc gcgctcgtgc gcgatttcct
    16441 actcgaagga gaatcgtcgt gaagaaaacc ttgatgctgg tcgccgtcct cgcaaccccc
    16501 gcatttgcac agcaacaatc cacgctcgac accgtgcgct cgaagggctt cgtccagtgc
    16561 ggcgtgaaca ccggcctcgc cggtttctcg cagccggact cgaagggcgt ctggaaaggc
    16621 atcgacgtcg acgtctgccg cggggtcgcc gcggcggtat tcggcgattc gagcaaggtg
    16681 cgctacaccc cgctcaccgc gcagcagcgc ttcaccgcgc tgcagtccgg cgaggtggac
    16741 atcctcgcgc gcaacaccac ctggaccatc acccgcgata ccagcctcgg cctcaacttc
    16801 gtcggcgtca actactacga cggccagggc ttcatggtcc accgcaagct caacgtgaag
    16861 agcgcgaagc agctgaacgg cgccaccgtg tgcgtgcagc ccggcaccac caccgagctc
    16921 aacctgtcgg actacttccg cgccaacaag atgagcttca agccggtggt gatcgagaag
    16981 atggaggaag tgctgaacgc gtacttcgcc ggacgctgcg acgtttacac caccgaccac
    17041 tcgggcctga tctcgacgcg cgcctcccgc gcgccgaagc ccgaagagca catcatcctg
    17101 cccgagatca tttccaagga gccgctcggg ccggcggtgc gtcacggcga cgatcgctgg
    17161 ttcgacgtgg tgaagtggac catgttcgcg atgctggaag ccgaggagat gggcatcagc
    17221 tcgaagaaca tcgacagcca ggccggcagc cagaacccga ccgtgcagcg cttcgtcggc
    17281 gcgagcggcg acatcggcaa gatgctcggc atcgacaaca agtggtcgtt caacatcatc
    17341 aagcaggtcg gcaactacgg cgagagcttc gacgcgaacc tgaagccgct cggcttcgag
    17401 cgcggcctga atcgcctgtg gaaccagggc ggcttgatgt acgcgcctcc gatccggtaa
    17461 acctgcaatg aagactgcgc ggcgacggct gctggctctt ggcctggcgc cgtttgccgc
    17521 accgatcagc acgtgggcgc aaccgcgcaa gccctggcgc atcggctatc tcgcgcaagc
    17581 cgatgccgcg gtgggcccgg acaccctcgg gcaactcaga gcgggaatga aggacctggg
    17641 ctacgaagaa aagcgcgact acctgctgga ggtgcgcaac gcgaaaggca gcccagagcg
    17701 gctggaccag tttgccgccg aactcgccgc gctgcgggtc gacctcattg tggcgtcctc
    17761 gactccgccg gccgtctccg ccctgaaagc cacgcgttcg atcccgatcg tgatggcgat
    17821 ctcgggcgat ccggtcggaa gcggcctgat caagagcttt gctcgtccag ggggaaacgt
    17881 gaccggcacc acgctcgcct tcgacgaggt gaaccgcaag tggttggaga tgctgatcgc
    17941 gatacgcccg ggcctgtcgc acgtgggcgt ggtttccaat ccggcgaacg tcagcatgcg
    18001 cggcatgctc gacccgctgg aggcatcgag ccgcgcgctg agcctgaagc tgactgtgca
    18061 ccacctgtcg ccgggcgagg atgcggaaac cctcctcggc aagctcaaac gcaaccctcc
    18121 cgaagggctg atcgtgctgc ccgacgcgca cattcgcacg tacgtcccgc agatcgcgca
    18181 gggccttgca cgcatgcggc tgcctgcggt ttacggcgtc gctgcctacg ccgagatggg
    18241 cgggctaatt agctatgggc ccgatcagcg cgcgcattac cggcgcgcag cggcgtatgt
    18301 ggagaaaatt ctcaagggag ccaacccggc cgaaatcccg gtggagttcc cgacgcggtt
    18361 cgagtttgtc gtgaacctca agagcgcgcg cgaggcgggg ctgagcattc cccatgagct
    18421 gctgttgcgc gcagacaagg tcatccggtg agccagaagc aaaaggcgga gagctttcgc
    18481 gcccttcaca agggcacctt tgtcatcgcc aacgcctggg acgcgggttc ggcgcgcatg
    18541 ctggccggcc tggggttcca ggcgctcgcg acctcgagcg gtgcgcacgc cggcaccctc
    18601 gggcgccgcg acggccaggt gacgcgcgaa gaggcgctgg aacatgcgcg cgcgatcgtg
    18661 gaggcaaccg acctgccggt atcggcggat ctagagagat gcttcgcgga cgagccggca
    18721 gcggcagcgg aaacgatccg cctcgctgcg ggcgtcggcc tcgtgggctg ctcgatcgaa
    18781 gatgccacca aggacaaggt ctacgagttc ggccatgcgg tcgagcgcgt cgccgccgcg
    18841 gtcaaggtcg cgcgctcgca tcatgtggtt cttacagcca ggactgaaaa cttcgtgcgc
    18901 ggcagacccg acctggatga cacgatcaag cgcctgcagg ccttcgagaa ggctggcgcc
    18961 gacgtcctca tggcgcccgg gcttcccgac ctcgaagccg tgcgcaaggt gtgcgcggcg
    19021 ctttccaagc cctacaactt catggccggc atcaagggca agtcgttcag cgtggccgag
    19081 ctccaggccg ccggcgtgcg gcgcatcagc ctcgccacct cgctctggcg cgcggcaatg
    19141 agcggcctcg tcgaggcggc gcgcgaggtg aaggacaagg ggaccttcgg ctacatcgaa
    19201 cgctcgctgg ccacgcccga catcagcaag tacatgaagt gaaggaggtg tacatcgagg
    19261 aggggtgctt tatcctcgag ctctcgaaca caccggacga tccggcggtc tcgatcgcgc
    19321 gggcgcgggt gccccggggc gtgacgaccc gctggcaccg ggtcaaggac acggcggagc
    19381 gctacgtgat cctggaaggc aaggggcggg cggaagtggg cgactcgccg ccgcgggagg
    19441 tcgggccggg agatgtggtg cggattccac cgtcctgtcg acagcgcata gccaacatcg
    19501 gcgacacgga cctggtcttt ctcgctattt gcacgccgcg gttcgttcag gaggcgtacc
    19561 agcagatcga atagcggctg aacgatcgac tgcagccgcg gtccagccct actggaggca
    19621 cacgtgcaac ctttctcgcc gcattatccc aggcccaatt ccccccgcat cgaggaatat
    19681 gccgcgatcc gtagggcgac cgtggcactc accgcgccgc tttcagccga agactgcgcg
    19741 ctgcagtcga tgtccgatgc cagcccggcg aagtggcatc tggcgcacac cacgtggttc
    19801 ttcgagacct tcgtgctcga gccgcacgtc cgtggctacc gcgccttcga tccggcgtat
    19861 cgcgttctgt tcaactccta ctaccacggt atcggcgacc ggcacccgcg cccggagcgc
    19921 ggcatgcttt cgcggccgtc gctcgacgag gtgctggctt accgaaacca tgtcgacgaa
    19981 gcgctcctcg cggcgaatga tcttcccgcc gcattgctcg agctcgggct gcatcacgag
    20041 cagcagcacc aggagctgat cctcaccgac gtcaagcatc tcctgtcgcg caatccgctc
    20101 aagcccgcct accagaagca ttggccgctc acgccgattc gcgcgcgagc gccgcgctgg
    20161 atcggattcg ccggcgggct gcacgaggtc gggcatgcgg gtccggggtt cgccttcgac
    20221 aacgaaacgc cgcgccatcg cgtgtggctg gaagatttcc agatcgcctc gcacccggtg
    20281 acgcacggcg atttcctcga cttcatcgac gacggcggct accggcggcc ggagctctgg
    20341 ctgtcggcgg gatgggatct ggtgcgcgcc cgcggctggc aggcaccgga gtactggctg
    20401 cgccgcgacg atcgctggca cacgttcacg ctccacggcg aggcgcccgt cgatcgcaac
    20461 acgccgatct gccacgtgag cttcttcgag gcggaagcct tcgcgcgctg ggcgggcgcg
    20521 cgcctgccga ccgaggcgga atgggagatc gccgcgcagg gcgcgccgcg cgagggcaat
    20581 tttgtcgaga gcggggcgct gcatccgctc gcgttgcgcg aggatccgtc gccgggaacg
    20641 ctggcgcagg cctacggcga cgtctgggaa tggacgcgca gcgactatgg tccgtacccg
    20701 ggctaccgcc ctgcgcccgg cgcggtcggc gagtacaacg gcaagttcat gtgcggccag
    20761 tacgtgctgc gcggcggatc ctgcgccacg ccggcgagcc acatccgcgc gacctaccgc
    20821 aacttcttcc cgcccgacgc ccgctggcag ttctccgggc tgcgcctggc gaaatagcgg
    20881 cttacagttt cgatcacctg gtaacccggg gtcgcgggag actgcgccat gaagtactgg
    20941 atagcgctcg cctgcttcgc aagcggcctc gcccacgcca acccggcgat cgacatggac
    21001 ggctacctgc gcgtctcgcg cgaggcggcg cagcaccgcg agacgcggcg cgtgtcggag
    21061 gcggaattca tccgcatgag ccgcgagccc ggcaccgtaa ttctggatgc ccgcagccgg
    21121 gagaagttcg acgagcttca cgtgaagcac gcgatcaacc tgcccttccc ggacatcgcg
    21181 atcgagagcc tcaggacaac catcccggac aggaacaccc ggatcctgat ctactgcaac
    21241 aacaacttcg cgaacgccga ggggccattc ccggccaaga tcgcgcgcgc ctcgctcaac
    21301 ctctcgacct acatcgcgct ctacagctac ggctaccgca acctctacga gctcgggccc
    21361 ctcatagacc tgaaagactc gagactcgag ttctctagtc gaccttagcg ccgacgtcgc
    21421 gcaccacctt gccccacttt gcgagctcgc tctttacata ggcggcggtt tcgctcaggg
    21481 gcgcggcggt gacgtcgaac gcgaggccct cgaggcgctc cttgatcagc ggatcgttgg
    21541 cgaccttgag aacggcatcg ttcagcttct gcgcgatttc cggcggtgtg ccggcgggga
    21601 cgaagaagcc gacccaggtg tagtcctcca tcccggggta gccgagctcg ccgagcgtcg
    21661 gcacgtcggg cagcgacgcg acgcggcgag ccgaagacac cgcgagcgcg cggatcttgc
    21721 cggccttgat atgcggcagc ggcgccgagc cggcgatcac gcccaccggc ggctggccgc
    21781 tcagcacggc ggccgcggcg ggaccgccgc ccttgaaggg aatgtgcgtg atgtcgagct
    21841 tcgcgcgcag cttgaacagg tgctcgccgg taaggtgcgg cgtggtgccg ttgcccgcgg
    21901 aagcgaaagc gagcttgtcg gtcttcgcac gcgccatgag ctcggccagc gtcttcgccg
    21961 ggaaatcggc gtgtaccacg atgaaattcg gttgcgaggc gacgatcgcg atcgggatga
    22021 aatccttctc gccgtcgtag cccgcgttgg gaatcagcgt ggggttgacc gcgtaggccg
    22081 aagaatgcgc gaggacggtg tagccgtccg gcgccgcctt cgcgacgcgc atggccgcca
    22141 cgttgccgcc ggcaccgacc acgttctcga cgaccacgct ctgcccgccg agcagctcgc
    22201 tcagcttcgg acccatcagc cgcgcgatga tgtccaccgg gccgcccggc gggaacgcca
    22261 ccgcgacgcg gatcggcttg gaaggatagg gttgcgcgaa tgccgtcgct gcagcaagca
    22321 gaaggaggga aaggacgatt ctcttcacga cgactcccta tttgatcgcc aggggattgg
    22381 tgggtgatcc taccgcgccc gtgatcggca gggcaggggc gacgaacagg aactcgtaca
    22441 ccttgtcggc ggcgcagtcc tcggcgagct ccttgaggta gaagatctcg cccatggtca
    22501 tgcccatgat cgggatggtg atccagtgcc agggctggtt gatgccggcc tcggactcgt
    22561 tcggccgcac ctcgcagccc caggtgtcgc ttgcgagcgc agcgagctgg gtgcgctgga
    22621 tccattccag cgtctcgaac gagaaccccg gcgcgtcgcc gcccgggtaa ccgtcccaac
    22681 ttccggcggc gagcttggct tccatctggc cggtccgcac gatgatgaag tcgccttttt
    22741 tcagctccac cttttgcagg gcggccgtcc tgtccagctc ggcaccggtg atcgcatgtc
    22801 cgtccggcag gatttccatg ccgagcgcgc gcggcacgtc gaggaacacg ccgcggccga
    22861 ccatccggtt cttggtcttt tcgatgccgc acttctgcgc gccggccgag gtcacctcgc
    22921 ggcagtcgta gccgttccac atgaagttct cgtagaacac gtgacccaag ccgtcccact
    22981 gggtgccgca ctgcaggggc atcaccacca tgtcgtcggc ggcgcggatg ccgcgcttgt
    23041 cgagcacgcc ggaataggcg tcggtgccgg tgcgcagcat ggtatggagc gggttgatgc
    23101 ggccgagcgc cggatacttg ctcttggcgc cctgcggccc gtgctggtcg aagttgagcg
    23161 cgagcgagat caccttgccc ttgcgcacca ggcgcgcggc ggcgacgatg tcttcgggcc
    23221 gggtgtggtt cagggtgccg atctcgtcgt gcggccccca gcgtccccag ttcttgtact
    23281 tctctgcggc ctttcgaaga tcgtctcggg taagcttgcg ctcggtcatg gtctggcgct
    23341 ctgcgagggg gaagcggata gcgtatcagg cggcggcaat cggcgccgcg gtcctggtcg
    23401 cgtggttcct ggtcagcaat acgattcaga atctggaaga gcgccgcatc gcgagcggct
    23461 tcggcttcct cggccgcgag gcgggcttcg agatcggcga gtcggcgttc ctgcgctaca
    23521 gcgcctccga cagctacctg cgggcgctct tcgtcgggct ctccaatacg ctcgcggtcg
    23581 cgctgatcgg catcgtgctc gcgaccggcc tcggcacgct gatcggtctt ctgcggctgt
    23641 cgaagaattt cctgctgaag acgctgagcg ccgcgtacgt cgagttcatc cgcaacgtcc
    23701 cgctcctggt gcagctcttc ttctggtatg cggtgatcac cgagggaatg ccggggccgc
    23761 gcgaggcgct gtcgccgctg cccggggtgt tcctgtccaa ccgcggcatc tacatcccga
    23821 ccttctcgag ctcgcccgag ttgcagggat tcaacttcgt cggcggcgcc gcgctcagcc
    23881 cggagttcgc cgcgctgctc atcgggctgg ttgcctatac cgcggcgttc attgccgaga
    23941 tcgtgcgcgc cggcatcctc gccgtggatc gcgggcaggg cgaggcggcg catgcgctcg
    24001 gcctgtcgcg cgggcagacg acgcgcctcg tcatcctgcc gcaggcgctg cgcgtcatca
    24061 tcccgccgat gaccagccaa tacctgaacc tcaccaagaa cagctcgctc gcggtggcga
    24121 tcggctaccc cgacctggtg tcgatcgcca acaccgccat gaaccagacc gggcaggcga
    24181 tcgaggggat cgcaatcatc atggcggtgt acctcacgat cagcctgtcg atctcggcgt
    24241 tcatgaactg gtacaacgcc cacgtcgctt tcaaatgaga tgggctagga caaatttgtt
    24301 cggctcgtgg ccggagacgg tcgccacgct gctcatcctg tggatcctgt ggcggaccgt
    24361 accgccgttc atcgactggg cgttcctcga cgcggtgtgg cgggccgatc cgaaggcgtg
    24421 ccgcgcgggc gagggtgcgt gctggggctt catcgccgag aagcaccgct tcatcctgtt
    24481 cggcacctat ccctacgagc tgcactggcg gccggccctg gcgacggtca ttctgatcgg
    24541 tctctgggtc ttttccgcgt tcagggtgtt ctggcgctcg tggctggtgc tcgtctggct
    24601 attcggcttg accgtcatcg gcgttctcat gtggggcggc gtgctgggga tgcagtacgt
    24661 cgagaacgag cgctggggcg ggctcatcct gacgctgctg ctctccacgt tcggcctggc
    24721 gatcgcgttt ccgctgtcga tcctgctcgc gctcggccgg cgctcggaga tgccggtgat
    24781 ccgggggctg tgcgtgggct acatcgagct gatccgcggc gtgccgctga tctcgctgct
    24841 cttcatggcc tcggtgatgc tgccgctgtt cctgccttcg ggagtgagca tcgacaagct
    24901 gctgcgcgcg cagatcgcga tgatcctgtt cgccgccgcc tacctcgccg aggtgatccg
    24961 cggcgggctg caggcgatcc cgagagggca gtacgacgcg gcgcatgcgc tcgcgctgcc
    25021 gttctggcgc agaacctacc tgatcatcct gccgcaggcg ctgcgcatcg cggtgcccgc
    25081 gctggtcaac aatttcatcg ccttcttcaa ggacacctcg ctcgtgctga tcatcggcct
    25141 gttcgacctg ctctcgacca tcaagatctc gctgcaggac cggacctgga ccggcttcgg
    25201 cgtcgaggcg tatctcttcg cctcgctggt ctatttcgtg ttctgcgccg ccatgtcgcg
    25261 ctacagcaag agcttcgagc accagcgatg atcgagatga tcaaggtgag caagtcgttc
    25321 ggcctgttcc aggcgctgac cgacgtcgac ctcgcggtgg cgcgcggcga gaaggtggtg
    25381 gtgtgcggcc cgtcgggctc gggcaagtcc acgctcatcc gcctggtcaa ccggctcgaa
    25441 gcgcaccaga gcggccgcgt catcgtcgac ggggtcgagc tcgacgacga cccgaagaag
    25501 gtcgacctgg tgcggcgcga ggtgggaatg gtgttccaga gcttcaacct gttcccgcac
    25561 ctcacggtgc tcgagaacct gacgctggcg ccgatctggg tgaagcacgt gccgcgcaag
    25621 gaggccgagg agaccgcgat gcacttcctc gcccgggtgc gcattcccga gcaggcgggg
    25681 aagtatccgt cgcagctctc cggcggccag cagcagcgcg tggccatcgc gcgcgcgctt
    25741 tgcatgaagc cgaagctgat gctgttcgac gagccgacct cggccctgga tccggagatg
    25801 atcaaggaag tcctggatgt gatggtggag ctcgcgcgcg agggcatgac catgatggtg
    25861 gtgacgcacg agatgggctt cgccaagacc gtcgccgacc gcgtggtgtt catggacggc
    25921 ggccgcgtgg tcgaggcggc gccgccggcg gagttcttcg gcgcgcccaa gaccgatcgc
    25981 gccaagctgt tcctcagtca ggtcctgggt cactaaggag aagaagatga aaacgctgct
    26041 gatcgccgta gccgttgctg ttctcggagt caatcaggcg gctgcgcagt cgtacccgag
    26101 ccggccggtc aggatcgtcg tgccggcgac cccgggcggg gcgatcgacc tgatcgcgcg
    26161 ctcgcttgcg gagaagctga cggcctcgct cggccagccg gtggtggtcg aaaacaagcc
    26221 cggcgcctcc aacaatctcg gcaccgactt cgtcgcgaag agccctcccg acggctacac
    26281 gctggtgatc gtggcgtcca gccacgccac caacaagcat ctcttcaagc agatgccgtt
    26341 cgatccgatc aaggatttcg agccggtggt ctacacgcac gtcgtgccgc tcctgctcgc
    26401 ggtgcatccc tcggtgcagg cgaaaaccgt tcccgagctg gtgggctgga tcaaggcgaa
    26461 cccggacaag gcgatctacg cctcgagcgg gccgggcagc tcgctgcaca tggcggccga
    26521 gctcttcatg agcatgaccg gcacgaagat gcaccacgtg ccctacaaag gaagctccgc
    26581 cgcgcatccc gacctgctcg ccggccgcac ggcgatgatc ttcgacacga tcacggcggt
    26641 gcgtggccac gtcaagtccg gcgcagtgcg cggcattgcg gtgacgacgg cgaagcgctc
    26701 aagcgcgatg cccgagctgc cgacgatcgc ggaaagcctg cccggctacg acgccagcac
    26761 ctggggcggg atcctcgcgc cggcgggaac gagcaaggaa gccgtctcga aattgaattc
    26821 ttcgatcaac gcggcgctca agctcgagga tgtccgctcg cgcctgatgg gcgccggcat
    26881 cgagatccag ggcggcacgc cggaggcctt cgccgaggtg attcgcatcg aagtggacaa
    26941 atggggccgc atcgtgaagg cggccggcat ccagccggaa tgacgctgcg cgagtgatcc
    27001 ggccctacgg ccggatggag tagacccgga cggtctcggc cttgccgcgc aaggcgacct
    27061 cgccgcgcga ttcgacccgg tcctggggca cgagccggcg gatttcctcg tagaagtcgc
    27121 tcgagaccag cacctcaccc atctgcgcag cctccatgag gcgcgccgcg gtgttcacgg
    27181 gattgccgat cacggtgtag ctcatgtagt gcggcgagcc cacgttgccg atgatcaccg
    27241 ggccctggca gatgccgatg ccgacgccg
//
LOCUS       DATBSZ010000249        20336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1958456, whole genome shotgun sequence.
ACCESSION   DATBSZ010000249 DATBSZ010000000
VERSION     DATBSZ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20336
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958456"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VIG70_15905"
     CDS             <1..658
                     /locus_tag="VIG70_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HEY8252083.1"
                     /translation="GWRLGTIAVHEDNIFDKMIAKHPESIKKKLDKRYGTLTLEPRKI
                     AFIDRIVADSRDVALNHTAGLSLPQQVMMTLFSAAELMDEKKDYQKACIGIVKKRVHA
                     TLEGLGLEVPPNPLFDFYYGLIDFEFWARKNLGEDIVTWMKKNVHPLDIVFRLAEEHG
                     IVLLNGSGFAAPDWSVRISFANLEDHVYDDIGRAVRSIAKGYRAAYEADKAKGKGKRK
                     "
     gene            669..1730
                     /locus_tag="VIG70_15910"
     CDS             669..1730
                     /locus_tag="VIG70_15910"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY8252084.1"
                     /translation="MKAKRLLALGTGLLSTTILAGVAQAQGVEVRLSGQINRAILHAD
                     DGAEKEWFNVDNGNSSTRFRFNAEGDISPGLKAGILFEAEFESNSSAAVSMSTRQVTP
                     VFEERHMDLFFRGGWGTVRLGQGDGAANGASEVDLSGTTVAHYASTPDVGGAFQYRSG
                     GALSGTSISATLSNQDFESRYDRLLYQSPSFSGFMAEASYGNKDTNIVEVAVRYSGKL
                     AALGTLAGAIGYSNEEAVAGGTDDKVLGGSISWLHTSGINLTYTHTNRDLPGRDGKFD
                     YFKAGYKFGKHAVSIDYASGQDQALSGDEAKMYGIGYVFTPIAWAEIFALLKQHSLDR
                     SGASLDDIQFFMVGSRIKF"
     gene            1730..1972
                     /locus_tag="VIG70_15915"
     CDS             1730..1972
                     /locus_tag="VIG70_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252085.1"
                     /translation="MRPILLACVMAAGCAAEPVAYHSGNEIPQGPGLLTGAEGAFVLR
                     AGEDKPRESAAPRNADEEREYREWREQKRQQENTKK"
     gene            1982..3193
                     /gene="gltS"
                     /locus_tag="VIG70_15920"
     CDS             1982..3193
                     /gene="gltS"
                     /locus_tag="VIG70_15920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEY8252086.1"
                     /translation="MTIELDLIHTILVSMVVLFAGRALVANVGVLSRFSIPAPVVGGG
                     LVAILLAFGDGLAGLKLSFNLGLKDSLLLMFFTTVGLAADARMLAKGGMKLVVFLAIS
                     AVLIVAQNVIGIGTALALDLHPVVGLLSGSITLVGGHGTGAAYAGRLGETLNLQGAME
                     LAMACATAGLVLGAVLGGPITEYLIKRHKLSGPKEDAGTEDTVAAKAAEDQVNSRTLI
                     NTLFVVLACLAGGKLLVKLVEQTGFILPDFVFCLLLGVAIRNVSAFTGLFKMSDRTIE
                     TVGDVALSLFLVMALMTMKLLDLANLAGPLLVILAAQAVVMILFTVFVTYRIMGSNYD
                     AAVIAGGQVGFGLSSTACALAIMKAVTLRHGASPLAFLIVPMVGAFFIDILNALVIQG
                     SLALPVFGFRP"
     gene            3190..4716
                     /locus_tag="VIG70_15925"
     CDS             3190..4716
                     /locus_tag="VIG70_15925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY8252087.1"
                     /translation="MKRLCLLILVLLNTACSRGPDEAALKTELQQRIDKSFKPGLLEL
                     AGLKRQGSSPLPAAESGAPRVVVYYNVTLRLKEGYDFKDWEGLSPATLANVLGGRERG
                     VIGVKAKENQPGDLLRVYGTSVYERSGDAWAAVAGAQRDVSQAPADPGSAAPATDAQR
                     YLERLASQVNIGPPGIDASSDKIISEELDHALRAINRRRARAQELVTVASGPAGGEYT
                     RVVEAVIGTLAKSKRKIRVIGVETEGSIENALLLGRGQADYGLVQSDVARLAFSGQGP
                     FAADGPLTKLAALGSLYPEPVHIVVPAQSAIRSIEDLRGKRVDIGTPQSGSRINAFAV
                     LQAHRIAIKDLAEARGAGPQAAMQQMRVGQLDAFFTTIGAPARELSRLAAGFPIRLLP
                     ISAAAAARIAAEQPGLVRLTLPANTYPGQSEPVATVAAAALLVGTVDTPQDEVKAVLE
                     LAFESTDYLAFGSAQGVKISKASGLRGIAIPLHPAAAEYFGAKPGAKPTAKPKPAAKK
                     "
     gene            4733..5437
                     /locus_tag="VIG70_15930"
     CDS             4733..5437
                     /locus_tag="VIG70_15930"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY8252088.1"
                     /translation="MRLASLAALLLIPAAVLAAEEPPQDELGTLLGAGVRSRPDYDGA
                     SKQELEPIPIIRYYGQTLFARTTQGILEGGARREFFPGFYAGIQLAYEAGNDRTDVDP
                     GASVGFHAEWDTRIGPAPFNLLGRIRYHLKEGYGVQYDLRGAVGVYGSSRLKAWVFGQ
                     ATWATDEWVERYYTAGGGGLLFTAFGVEGAYDLSRHWVVLASLSLRRLHDDAASSPIT
                     EDKTSYYASAGVAYRF"
     gene            5449..6327
                     /locus_tag="VIG70_15935"
     CDS             5449..6327
                     /locus_tag="VIG70_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252089.1"
                     /translation="MRKVRVAPKGMGLNDFVAQQEGFFAAEGIEVEWDIKTFRGTQSS
                     WKDLDYGSRPQDKPYTEEKTAGLIQCACVWGSISNASAGMGRFVPDCYGVSPWAIFVR
                     PDSKIRKPQDLKDVPVSVGMRAGSHFNVPFRLEKYLPLENIKVINSGGFGARLKALLD
                     KEVEAASLLPPQIDMARQLGLRMVMEDEFHTLWWVPETADPDDVRAYLNGLHRAEQAL
                     QADLKKYLPLWEKCVPPEFKDIHKWDYAKFTRGERFVYQPIPRAEFDQTLEQVKRWDL
                     DQYLKEKSFERLTYRV"
     gene            complement(6324..6602)
                     /locus_tag="VIG70_15940"
     CDS             complement(6324..6602)
                     /locus_tag="VIG70_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252090.1"
                     /translation="MAPRAVLVALIVFLTGCAAPVTQWEKPGASPTAVDEAMQQCRVE
                     TRLAPQQHLPPTPRTMGTPGMDRLESRDAREAEQFQKCMRDKGYTPKS"
     gene            6650..6913
                     /locus_tag="VIG70_15945"
     CDS             6650..6913
                     /locus_tag="VIG70_15945"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEY8252091.1"
                     /translation="MDAAGDKMMKELREVVAAAEELLSAATPSAEQMKEIRDRAEDAL
                     RGARERLEGAGDEIEERVRKHPFAALGIAAAVGLVVGILLSRK"
     gene            6910..7275
                     /locus_tag="VIG70_15950"
     CDS             6910..7275
                     /locus_tag="VIG70_15950"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY8252092.1"
                     /translation="MTAGLLQSAQDFVARLLDLGRTRFELFGTELREELAHLATTVLG
                     GLAVLVLAALGLAFCGLALIFFVSEANRLAATIGVAVFFLVVAGVLAWVLQRVRGTKP
                     RAFDATIAELRRDLDAIKP"
     gene            7272..7529
                     /locus_tag="VIG70_15955"
     CDS             7272..7529
                     /locus_tag="VIG70_15955"
                     /inference="COORDINATES: protein motif:HMM:NF025363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HEY8252093.1"
                     /translation="MTQREQGLAARRRELVERSSAQRAALAAAADPLVRKSAALDRVV
                     AQVRRYPVVASVAVGALALLGPRRLFDLAARAATLYALLRR"
     gene            complement(7530..10400)
                     /locus_tag="VIG70_15960"
     CDS             complement(7530..10400)
                     /locus_tag="VIG70_15960"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEY8252094.1"
                     /translation="MNKLVARLRARRILLGVSALLLAAYAAAGFFLVPYIARSQIEAS
                     VAETLQRRIAIGEIRFNPFTLAATLIDLKLTEADGAPLVSFRSLYVNAEISSLWRRGV
                     VFKEIDLDAPDIEVIVAPDGSLNLARLAPPAAPAQDKPKTDEAPLRVHIGRFSVEDGR
                     IGVQDRSLAQPFNVAFTPIRFSLRDFRTDVGHSNEYSFTTSSRIGARLEWSGAFTVQP
                     LGSRGTFSVADLRLAALDDYLEDKLPAEMVSGSLQFRGSYKFELNPLSLEVALPSIGV
                     RDLVLAERGVAASAPIALPEIDVHDVAFSLSRRDVGVRRVDVRGARIEVVREPDGGIN
                     LARFAGGSPAPAESPKSSEAPWTVHADAISLETATVVIEDRTLSPAARLQLTPAVMIN
                     GFTTARDSRMKVDVRIGIDGKGHLGVKGDVGLEPLGAALAIDLQKFPLPSLQPWVTQA
                     TAIKLHSGSLGLKGNLNFTPAASKFSGGLSIDDVRASDEVVREDLLKWRTLAITGIQF
                     QQRPDRLRIERIVAREPYARVIIAKDGGVNIANALKPRAGAEASPAPAAQEKPAAEKK
                     PLLIAIRTVELVDGSANFADYSVQPDFAIGIVGLGGQITGLSSAPESRAKVALNGRVD
                     EYSPVDVSGEVNVLSAALYTKLALNFRNIELTAFNPYAGKFAGYSIAKGKLSTQTQYH
                     VEQGKLDAKHHISVDNLEFGDRTDSKDAAPIPIKLGVALLKDRRGMIEVDLPVSGTLD
                     DPEFHLGSIIWKSMLSVLANVIRAPFAALGSLFGSGEDLAFVEFQPGSAVLGDAETRK
                     LETLAKGLVERPVLRLSVPLTVATAADSDATARQALAALVPPAESAAGVDEDAKRKRI
                     EALEGAYRMQLKTEPAYLADAKSAEDPNLDARIGWLQAALLEDLKPGPAALEALGKQR
                     AGAVRDALLANKDVNPERVFIVAKPAEAASPAGLVRMEMKLE"
     gene            complement(10484..10888)
                     /locus_tag="VIG70_15965"
     CDS             complement(10484..10888)
                     /locus_tag="VIG70_15965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEY8252095.1"
                     /translation="MASDEQQIRQIMEEWRRRTAEGDVEGLLSLTADHAVFLTPGNPP
                     ITKTAFAEGFRRISAKARIEPRQQLKDLGICDDLAYAWSHLTVVLTAKRDGARTERSG
                     HVLTVFRKNAAGTWQLARDANLMAGAGNPERA"
     gene            complement(10967..11710)
                     /locus_tag="VIG70_15970"
     CDS             complement(10967..11710)
                     /locus_tag="VIG70_15970"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8252096.1"
                     /translation="MPLQGLKGRKALVTGAASGIGDAVAKRLAGEGVEVFATDIHPAP
                     NLLVADLTDADDIEKLSAEVGTPDFLINIAGGPAAPTRGDTLPPIPGAIPLEDLEEKD
                     WSAVIAANLTTAFLVCREFAPRMKARKFGRIVNFASIAARRGSDRVGVHYAAAKGGII
                     GLTKTLALELGPHGITVNAIAPGFIKTPRIEAAGWGDAGAAFIESLPLKRAGKPDDIA
                     GVVAMLCSEAGGYVSGATIDVNGGWYFGP"
     gene            complement(11730..14066)
                     /locus_tag="VIG70_15975"
     CDS             complement(11730..14066)
                     /locus_tag="VIG70_15975"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY8252097.1"
                     /translation="MPRNLRRLAAIFLSAIVAVPAAAVLYLAFGDLSRHKGRIEALVA
                     QQTGRSFAIDGALELQVLPSISVVAERVRLANADWGSQPQMIELGRVSVRIGLWSLFS
                     GPVDVRSFEVRDLSVLLETGPEGQGNWVLGAASAPEEAAAEPGASAPALPPVIQNGKL
                     ANVRITYRERGKPDRVAFVETFAIGPGTAGLLAISGKGSLDEFPASVSGELGPLDALF
                     SGRDIRIAIEAGVGDLRLDVKGGIGRFDPLDGADLAVKLGHPDIGAMLKKLDLPVIAS
                     GALVAEVKLADAGDLTRLDVAAKLADITLSVEGTLRALGLPGSDLRVKGSVANLERLA
                     AALDVAGLPAGALEFGGRLTASRAEINLDGMNARFAGARARVDGTLRPARGSADLRFE
                     LAAESLAKLRAGLPETPLSMSGTYAGSRDKLEVKSLKGKIGESEFSARASMAGSKKKR
                     VDIELASPRLDLTPLVPDSKAKPKPKPKEAARKYVFDEAPLPLDKLKLADAKLRFSSA
                     EVRLGTEILRDVESTLLLEGGRLTLEGRATDNFGGTIRSAVKLTPADDGSGALSISVS
                     AKNVRFSLGAGDAIDPKDAPPTSVEASLLVKGASARQMAAGANGRVVVTQGAGKLSTG
                     VVGLIGGDLLRELAGKLNPFSAQDPYTQLECTVVRADILDGQVAVKPVLMQSVKVTIV
                     AGGKVDLGTEALRFDFNTRPRTGVGITAGMFANPFIELAGTLASPKLGVGAKGATSGA
                     AAVATGGISVFAQGMLDRVRGTQDLCKKTLEESAAKSK"
     gene            complement(14056..14838)
                     /locus_tag="VIG70_15980"
     CDS             complement(14056..14838)
                     /locus_tag="VIG70_15980"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8252098.1"
                     /translation="MKVAVVTGAAKPESIGHEVARRLLKEGMQVVVADLYEEGFSALR
                     ECFCVRCDVSDPSSVQEMIEKAVKRFGRIDVLVNCVGGSWGITRKDLVAAPPLGFRGL
                     TNCSLEDWRTIVAANLDSVFYCARAAAPHLKKQGGAIVNVASVAARKGIPPGSEGSSG
                     PYAVAKAGVMGLTRQLALELGPFGVRVNCVAPGVIASGRLKKVLGITKQDHGPEGSLK
                     TIPMGRVGTVDECAALIVALCTDDTSYMTGVTVDANGASYAA"
     gene            complement(14860..15699)
                     /locus_tag="VIG70_15985"
     CDS             complement(14860..15699)
                     /locus_tag="VIG70_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8252099.1"
                     /translation="MPGALIDGGRKVAALPGFASVLEIVEGGQNALDRAYESIKRSPT
                     VLEVGNVTLLAPIPHPPQMRDFLCFEEHLVQAFRAIGKSPPQAWYERPIFYHPSRFSV
                     CGPEAEVPWPAYSERLDFELEFGCYIGRPGKDIPREQARSHIFGYTIFNDFSARDEQA
                     KEMAGQLGPGKGKDFDNANAMGPCLVTADELGDPYRLEMAVRVNGEEWGRGNSRDMHW
                     KFEDCIAHASRSETLHPGEFFGSGTVGNGCGLEQMKFLKPGDVVELEVEGIGVLRNRV
                     VRG"
     gene            15793..16710
                     /locus_tag="VIG70_15990"
     CDS             15793..16710
                     /locus_tag="VIG70_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEY8252100.1"
                     /translation="MRQFNEDTLTNEVVARLKKTKNPRLREIMTSLVKHVHAFAREVH
                     LTEEEWFEGIKFLTSVGQKCDGKRQEFILLSDVLGLSMMVVALNHKTQAGATEATVLG
                     PFFAHGAKEFGYGADLREGATMKGEDVWVSGRVLSVDGKPIPNAALDIWQAKADGIYD
                     LQTEGEFELRGRVKANAKGEFAFASYKPNFYSIPIDGPVGDMVRATTGNHMRPAHMHA
                     IVSAPGYQQVITHVFVEGDPHLDGDAVYAVKNSLIGKYKKVSNPEQAKKRGLPNPFLT
                     LTWDFRLAADKAGAKKARKKIAASDAVAT"
     gene            16707..17288
                     /locus_tag="VIG70_15995"
     CDS             16707..17288
                     /locus_tag="VIG70_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8252101.1"
                     /translation="MIWRVRRVLTGHDAEGRSTFIADGEAPNVKEMTAITGLALTDLW
                     ETGAAPASNLGLKDAADRPVHLEPPKNGTILRIVEFPPDSAWRGATDGSAGFKAIGAG
                     HAQDKSSSDPMMHKTATVDYIIVLKGEIYAIMDAGEKLLRAGDVLVQRGTNHSWSVRS
                     NEPCIIAAVLVSAKPVSAPSTKKPSKKSKKKKK"
     gene            17415..18263
                     /locus_tag="VIG70_16000"
     CDS             17415..18263
                     /locus_tag="VIG70_16000"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8252102.1"
                     /translation="MGVIGLGIMGSAMSANLLKAGFDVRGYDIVASRRAALKRAGGKP
                     ASSLSRLNAEVLITSLPSAEALHAVSQQLKRKCIVVETSTLPIEDKVKARDALKRKGI
                     TLLDCPLSGTGAQAKTGDLVVFASGDRKAFTKAKSIFPGFSRAHHYLGEFGNGSKMKY
                     VANLMVAIHNVSAAEAFVLGMKAGLSPKAIYEVQANGAGTSRMFQVRGPMMVAGRYGD
                     VTMKNEVWQKDMKIIGEFAARLGVPTPLFNASAAIYSAAMAQGFGKQDTGAVCAVLES
                     MAKHRR"
     gene            complement(18329..18706)
                     /locus_tag="VIG70_16005"
     CDS             complement(18329..18706)
                     /locus_tag="VIG70_16005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8252103.1"
                     /translation="MTEARSKRLLVADDDADMRLTLKLALEIAGYAVDVAANAREALA
                     LQRGQPADVLITDIFMPDADGFEAIDAFRHEFPRTRIIVVSGGAQFTKRDYLPDAELI
                     GVDATLQKPFDIDTLLATLRSLQ"
     gene            complement(18703..>20336)
                     /locus_tag="VIG70_16010"
     CDS             complement(18703..>20336)
                     /locus_tag="VIG70_16010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8252104.1"
                     /translation="DVLRAQRLPESWVIALVDRNRRFIARLPPMAPGRAVSESFRAAI
                     ERSPNGWFPGSTLEGTRTYTPYVTSELSGWVLGIAIPARSVEAAATRTFFGFAAGIAT
                     VLALALLLGWYMARRIASPIAALAAATEAMGQGKDVRVADPGRIDEISRLYEALRAAA
                     AAVRERSELQEREKTALQAADRSKDEFLAMLSHELRNPLAALTAAAHVLKFAQPGDQA
                     AAKARAVVERQTKHMARLVGDLLDISRVAMGKVALERERFNLAEAAGNLLNVWRASGR
                     LERHHVSVEMKAAWVDADRARIEQVLSNLLDNAVKFTPAGRRIAVRVGPEGEWAQLCV
                     SDEGEGLAPGSAERMFDLFVQGERGLDRAAGGLGVGLALVKRLTEMHGGSVAGESAGP
                     GQGATFTVRLPAVLAPSAAAAPAALEIAPRMQPRRVLIVEDNDDTRQMLHETLAFSGH
                     EVREARDGASGLALAAEARPDVALIDIGLPDLDGYEVARRLRAAPGGRRIGLIAITGY
                     GQSEDQRRAYEAGFDAHLTKPVAPERLKQVMAGLR"
BASE COUNT         3309 a         7002 c         6812 g         3213 t
ORIGIN      
        1 cggctggcgc ctcggcacga tcgcggtgca cgaggacaac atcttcgaca agatgatcgc
       61 gaagcacccc gagtcgatca agaagaagct ggacaagcgc tacggcacgc tgacgctcga
      121 gccgcgcaag atcgcgttca tcgatcgcat cgtggccgac agccgcgacg tcgccctgaa
      181 ccacaccgcg ggcttgtcgc tcccgcagca ggtgatgatg acgctgttct cggcagccga
      241 gctgatggac gagaagaagg actatcagaa ggcctgcatc ggcatcgtca agaagcgggt
      301 ccacgcgacg ctggaaggcc tcggcctcga ggtgccccca aacccgctgt tcgacttcta
      361 ctacgggttg atcgacttcg agttctgggc gcgcaagaac ctgggcgagg acatcgtgac
      421 gtggatgaag aaaaacgtgc atccgctcga catcgtgttc cgcctggccg aggagcacgg
      481 catcgtgctc ctcaacggca gcggcttcgc cgcgcccgac tggtcggtgc gcatttcgtt
      541 cgcgaacctc gaggaccacg tctacgacga catcggccgg gcggtgcgct cgatcgccaa
      601 gggctaccgg gcggcctatg aggcggacaa ggccaagggc aaaggaaaac ggaaatagga
      661 aaacggaaat gaaagcgaag cgtctgctgg cgctcggcac cggcctgctg tccaccacca
      721 tcctcgccgg cgtcgcgcaa gcccagggag tcgaggtcag gctctcgggc cagatcaacc
      781 gcgccatcct ccacgccgac gacggcgcgg agaaggaatg gttcaacgtc gacaacggca
      841 acagctcgac gcgcttccgg ttcaacgccg agggcgacat cagccccggc ctgaaggccg
      901 gcatcctgtt cgaggccgag ttcgagtcga actcgagcgc cgcggtctcc atgagcacgc
      961 gccaggtcac gccggtcttc gaagagcggc acatggacct gttcttccgc ggcggctggg
     1021 gcacggtgcg gctcggccag ggcgacggcg cggcgaacgg cgcctcggaa gtcgatcttt
     1081 ccggcaccac cgtcgcacac tacgcgtcca cgcccgacgt cggcggcgcg ttccagtacc
     1141 gctcgggcgg cgcgctctcg ggcacgagta tcagcgccac gctctccaac caggacttcg
     1201 aaagccgcta tgaccggctg ctctaccagt cgccctcgtt cagcggattc atggccgagg
     1261 cgagctatgg caacaaggac accaacatcg tcgaggtggc ggtgcgctac agcggcaagc
     1321 tcgccgcgct cggcaccctc gccggcgcga tcggctactc caacgaggag gccgtggccg
     1381 ggggcacgga cgacaaggtc ctgggcggct cgatctcctg gctgcacacg agcggcatca
     1441 acctgaccta tacccatacc aaccgcgacc tgccggggcg cgacggcaag ttcgactact
     1501 tcaaggcggg ctacaagttt ggcaagcacg ccgtgtcgat cgattacgcg agcggccagg
     1561 accaggcgtt gtccggagac gaagcaaaga tgtacggcat cggctacgtc ttcacgccga
     1621 tcgcctgggc cgagatcttc gccctcctca agcagcacag cctcgaccgc tcgggcgcga
     1681 gcctcgacga catccagttc ttcatggtcg gctcgcggat caagttctga tgcgccctat
     1741 tctcctcgct tgtgtgatgg cagcggggtg cgccgccgag cccgtcgcgt accattcggg
     1801 caacgagatc ccgcaggggc cggggttgct caccggagcc gaaggggctt tcgtgctgcg
     1861 ggcgggagaa gacaagccac gcgagtcggc cgcgccgcgc aacgccgacg aagagcgcga
     1921 gtaccgggag tggcgcgagc aaaagcgcca gcaggaaaac acgaaaaaat aggacggccg
     1981 tttgacgatc gagcttgacc tgatccacac gatcctcgta tcgatggtcg tgctgttcgc
     2041 cggccgcgcg ctggtggcga acgtcggcgt gctttcgcgc ttcagcatcc ccgccccggt
     2101 ggtcggcggc ggcctggtgg cgatcctgct cgcgttcggc gacggcctcg ccggcctcaa
     2161 gctgtcgttc aacctggggc tcaaggactc gctgctgctc atgttcttca ccaccgtcgg
     2221 cctcgccgcc gacgcgcgca tgctggcgaa gggcggcatg aagctggtgg tgttcctcgc
     2281 catcagcgct gtgctgatcg tggcgcagaa cgtcatcggc atcggcactg cgctcgcgct
     2341 cgacctgcac ccggtggtcg gcctgctgag cggttcgatc accctggtcg gcgggcacgg
     2401 caccggcgcg gcctacgccg ggcgtctcgg cgagacgctg aacctgcagg gcgcgatgga
     2461 gctcgccatg gcgtgcgcca ccgcgggcct ggtgttgggc gccgtcctgg gcggcccgat
     2521 caccgagtac ctgatcaagc gccacaagct gtccggcccg aaagaggatg ccggcacgga
     2581 agacaccgta gcggcgaagg cggcggagga ccaggtcaac tcgcgcacgc tcatcaacac
     2641 gctgttcgtc gtgctcgcct gcctcgcggg cggcaagctc ctcgtaaagc tggtcgagca
     2701 aaccggcttc atcctgcccg acttcgtctt ctgcctgctg ctcggcgtgg cgatccgcaa
     2761 cgtctcggcg ttcaccggcc tcttcaagat gagcgaccgc acgatcgaga cggtgggcga
     2821 cgtggcgctg tcgctcttcc tggtgatggc gttgatgacg atgaagctcc tcgacctcgc
     2881 caacctcgcc ggcccgctgc tcgtaatcct cgccgcgcag gccgtggtga tgattctttt
     2941 caccgtgttc gtcacctacc ggatcatggg cagcaactac gatgccgcgg tgatcgccgg
     3001 cggccaggtc ggcttcggcc tctcctcgac cgcgtgcgcg ctcgccatca tgaaggcggt
     3061 gaccttgcgc cacggcgcct cgccgctcgc tttcctcatc gtgccgatgg tcggggcgtt
     3121 cttcatcgac atcctgaacg cgctcgtcat ccagggctcc ctcgccctgc ccgtcttcgg
     3181 attccggcca tgaaacgcct ttgcctcttg atattggtgc ttctcaatac agcttgttcc
     3241 cgcgggcccg acgaggcggc gctcaagacc gagctgcagc agaggatcga caagagcttc
     3301 aagcccgggc tgctcgagct cgccgggctc aagcgccagg gctcgagccc gctgccggcg
     3361 gccgagagcg gtgcgccgcg cgtcgtggtg tactacaacg tcaccctgcg gctgaaagag
     3421 ggctacgact tcaaggactg ggaaggcctg agcccggcca ccctggccaa cgtgctgggc
     3481 gggcgcgagc gcggcgtgat cggcgtgaag gcgaaggaga accaacccgg cgacctgctg
     3541 cgcgtctacg gcacctcggt ctacgagcgc tccggggacg cctgggccgc ggtcgccggg
     3601 gcgcagcgcg acgtctcgca ggcgcccgcc gatcccggca gcgccgcgcc ggctaccgac
     3661 gcgcagcgct acctcgagcg cctcgcctcg caggtcaaca tcgggccgcc gggcatcgac
     3721 gcgagctcgg acaagatcat ttcggaagag ctcgaccatg cgctgcgcgc gatcaaccgg
     3781 cggcgcgcgc gggcgcagga gctggtgacc gtcgccagcg gtcccgcggg cggggaatac
     3841 acgcgcgtgg tggaagcggt catcggcacc ctggccaaga gcaagaggaa gatccgcgtg
     3901 atcggcgtcg agaccgaggg cagcatcgag aacgcgctgc tgctcgggcg cggccaggcc
     3961 gactacggcc tggtgcagag cgacgtcgcg cggctcgcct tctcgggcca gggtccgttc
     4021 gcggcggacg gcccgctcac caagctggcg gcgctcggca gcctctatcc cgagcctgtg
     4081 cacatcgtgg tgcccgcgca gtccgcgatc cgctcgatcg aggacctgcg cggcaagcgc
     4141 gtggatatcg gcacgccgca gtcgggcagc cggatcaacg ccttcgccgt cctgcaggcg
     4201 caccgcatcg cgatcaagga cctcgccgag gcgcgcggcg ctggccccca ggcggcgatg
     4261 cagcagatgc gcgtcggcca gctcgacgcc ttcttcacca ccatcggcgc gccggcgcgc
     4321 gagctgtcgc ggctggcggc ggggttcccg atccgcctcc tgcccatctc ggcggctgcc
     4381 gcggcgcgca tcgccgccga gcagccaggg ctggtgcgtc tcacgctgcc ggcgaacacc
     4441 tatcccggcc agagcgagcc ggtcgcgacc gtcgcggcgg ccgcgctgct cgtcggcacc
     4501 gtagacacgc cgcaggacga agtgaaggca gtgctcgagc tggcgttcga gagcaccgac
     4561 tacctcgcct tcggcagcgc gcagggcgtc aagatctcca aggcgagcgg gctgcgcggc
     4621 atcgcgattc cgctgcatcc cgccgcggcg gaatacttcg gcgcaaagcc cggtgcaaag
     4681 cccaccgcca agcccaagcc cgcggcgaag aagtagttag tattcgaggg tcatgagact
     4741 tgcctcgctg gcagcgctgc tgctcattcc cgccgccgtg ctcgcggccg aggaaccgcc
     4801 gcaggacgag ctcggcacgc tcctcggcgc cggcgtgcgc tcgcgcccgg attacgacgg
     4861 cgcctccaag caggagctcg agccgattcc catcatccgc tactacggcc agacgctctt
     4921 cgcgcgcact acgcagggca tcctggaagg cggtgcgcgc cgcgagttct tccccgggtt
     4981 ctacgccggc atccagctcg cctacgaggc cggcaacgat cgcaccgacg tcgaccccgg
     5041 ggcctccgtc ggcttccacg ccgaatggga cacccggatc gggccggccc ccttcaacct
     5101 gctcggccgg atccgctatc acctgaaaga gggctacggc gtgcagtacg acctgcgcgg
     5161 cgcggtcggc gtctacggca gcagccgcct gaaagcctgg gtcttcggcc aggcgacttg
     5221 ggcgaccgac gagtgggtgg agcgctacta caccgccggg ggcgggggac tgcttttcac
     5281 cgcgttcggc gtcgagggcg cgtacgactt gagccggcac tgggtcgttc tcgcgagcct
     5341 ttccctgcgc aggctccacg acgacgccgc gtcgagcccg atcaccgagg acaagaccag
     5401 ctactacgcg agcgccggcg tagcatatcg gttctgaacg gaggaaccat gcgcaaagtc
     5461 cgcgtcgccc cgaagggcat ggggctcaac gatttcgtgg cgcagcagga aggcttcttt
     5521 gctgccgagg gcatcgaggt cgaatgggac atcaagacct tccgcggcac gcagtcgagc
     5581 tggaaggacc tcgactacgg gtcgcggccc caggacaagc cttatacgga agaaaaaacc
     5641 gcggggctga tccaatgcgc ctgcgtctgg ggcagcatct ccaacgcctc cgccggcatg
     5701 ggccgcttcg ttcccgactg ctacggcgtt tcgccgtggg cgatcttcgt gcgccccgat
     5761 tcgaaaatca gaaaaccgca ggatctcaag gacgtccccg tttcggtcgg catgcgcgcc
     5821 ggcagccact tcaacgtgcc cttccgcctc gagaaatatc tgccactgga aaacatcaag
     5881 gtaatcaaca gcggcggctt cggcgcgcgc ctgaaggcgc tgctcgacaa ggaagtggag
     5941 gcggcgagcc tgctgccgcc gcagatcgac atggccaggc agctcggctt gcgcatggtg
     6001 atggaagacg agttccacac gctgtggtgg gtccccgaga ccgccgaccc ggacgacgtg
     6061 cgggcttatc tcaacgggtt acatcgtgcg gagcaggcgc tgcaggccga tctgaagaaa
     6121 tatctcccgc tgtgggagaa atgcgtgccg ccggagttca aggacatcca caagtgggac
     6181 tacgcgaaat tcacgcgcgg cgagcgcttc gtctaccagc cgatcccgcg cgcggagttc
     6241 gaccagacgc tcgagcaggt gaagcgctgg gatctcgacc agtacctgaa ggaaaagtcc
     6301 ttcgagaggc tgacctaccg cgtctaggac ttcggggtgt agcctttgtc gcgcatgcat
     6361 ttctgaaact gctcggcctc gcgcgcgtcg cggctctcga gccggtccat gcccggcgtg
     6421 cccatcgtgc gcggagtcgg gggcaggtgc tgctgtggcg ccagacgcgt ctccacgcgg
     6481 cactgctgca tggcttcgtc gaccgcggtc gggctggcgc cgggcttttc ccactgcgta
     6541 accggcgctg cgcaacccgt cagaaagacg atcagggcga caaggacagc gcgcggagcc
     6601 atcggatgca aaggtatcat tgtcccggac gatcctgaag gaggtccgca tggacgcagc
     6661 cggtgacaag atgatgaagg aactgcgcga agtcgtggcc gcggccgagg agcttctctc
     6721 cgccgcgact ccgagcgccg agcagatgaa ggagatccgc gaccgcgccg aggacgcgct
     6781 gcgcggcgcg cgcgagcggc tcgagggcgc gggcgacgag atcgaggagc gggtgcgcaa
     6841 gcatcccttc gccgcgctcg gcatcgccgc ggccgtcggc ctggtcgtcg gcatcctgct
     6901 gagccgcaaa tgaccgccgg cctgctgcaa tcggcgcagg acttcgtcgc ccggctgctc
     6961 gacctcgggc gcacccgctt cgagctcttc ggcaccgagc tgcgcgagga gctcgcgcac
     7021 ctggccacca ccgtgctcgg cgggctcgcg gtgctggtgc tcgccgcgct cggcctggcg
     7081 ttctgcgggc tcgcgctcat cttcttcgtg agcgaagcga accgccttgc ggcgacgatc
     7141 ggcgtcgcgg tgttcttcct cgtcgtggcg ggcgtgctcg cttgggtgct gcagcgcgtc
     7201 aggggcacca agccgcgcgc gttcgacgcc acgatcgccg agctgcggcg cgacctggac
     7261 gccatcaagc cgtgacccag cgcgagcagg gtctcgccgc gcgccgccgc gagctcgtgg
     7321 agcgctcctc cgcccagcgc gcggcgctcg ccgccgccgc ggatccgttg gtgcgaaagt
     7381 ccgcggcgct cgaccgcgtc gtcgcccagg tgcggcgcta tccggtggtg gcctcggtcg
     7441 ccgtcggcgc gctcgcgctc ctcggcccgc gccgcctctt cgacctcgcc gcgcgcgcgg
     7501 ccacgctgta cgcgctcctc aggcgctaat cactcgagct tcatttccat ccgcaccagg
     7561 cctgccggag acgcggcttc ggccggcttc gccacgatga agacccgctc cggattcacg
     7621 tccttgttcg caagcagcgc atcgcgcacc gcgcccgcgc gctgcttgcc caaagcctcg
     7681 agcgcggcgg gcccaggctt gagatcctcg agcagggcgg cctgcagcca gccgatcctc
     7741 gcgtcgaggt tcgggtcctc cgcgctcttc gcgtcggcga ggtacgcagg ctcggtcttg
     7801 agctgcatgc gataggcgcc ctcgagcgcc tcgatgcgct tgcgcttcgc gtcttcatcg
     7861 acgcccgccg cggactcggc aggcggcacg agcgcggcca gcgcctgcct tgctgtcgcg
     7921 tcgctgtccg cggccgtcgc aacggtgagc ggcacgctca ggcgcagcac cggccgctcg
     7981 accaggccct tggcgagcgt ctccagcttc ctggtttccg cgtcgccgag cacggcggag
     8041 cccggctgga actccacgaa cgccaggtct tccccgctgc cgaacaggga gccgagcgcc
     8101 gcgaacggcg cgcggatcac gtttgcgagc acggacagca tgcttttcca gatgatcgac
     8161 ccgaggtgga attccggatc gtcgagcgtg ccgctcaccg gcaggtcgac ctcgatcatg
     8221 ccgcggcgat ccttgagcag cgccacgccc agcttgatcg gaatgggcgc ggcgtccttc
     8281 gaatcggtcc ggtcgccgaa ctcgaggttg tcgacgctga tgtggtgctt cgcgtcgagc
     8341 ttgccctgct cgacgtgata ctgggtttgc gtcgacagct tgcccttcgc gatgctgtag
     8401 cccgcgaact tgccggcata cgggttgaac gccgtcagct cgatgttgcg gaaattcagc
     8461 gcgagcttcg tgtacaaggc cgccgagagc acgttgacct cgccgctgac gtcgaccggt
     8521 gaatactcgt ccaccctgcc attcagcgcc actttggcgc gcgattcggg cgccgatgac
     8581 agcccggtga tctgcccgcc caatccgacg atgccgatgg cgaaatcggg ctgcaccgag
     8641 tagtcggcaa agttcgcgga gccatcgacg agctcgaccg tgcggatcgc aatcagcagg
     8701 ggcttcttct ccgccgcggg tttctcctgc gccgcgggcg cggggcttgc ctcggctccg
     8761 gcgcgcggct tcagggcgtt ggcgatgttg acgccgccgt ccttggcgat gatcacgcgc
     8821 gcgtagggct cgcgcgcgac gatgcgctcg atgcgcagcc gatccggccg ttgctggaac
     8881 tggatgccgg taatcgccag cgtgcgccat ttcagcagat cctcgcgcac cacctcgtcg
     8941 ctcgcgcgca cgtcgtcgat gctcagcccg ccggagaatt tcgacgccgc cggcgtgaaa
     9001 ttcagattgc ccttgaggcc caggcttccc gaatggagct tgatcgccgt cgcttgcgtc
     9061 acccagggct ggagcgacgg aagcgggaat ttctgcaggt cgatggcgag cgcagcgccc
     9121 agcggctcca ggccgacgtc gcctttcacg cccaggtgtc ccttgccgtc gattccgatg
     9181 cgcacgtcca ccttcatgcg ggaatcgcgc gccgtcgtaa aaccgttgat catgacggcg
     9241 ggcgtgagct gcaggcgggc cgcgggcgac agggtgcggt cctctatgac cacggttgcc
     9301 gtctcgagcg agatcgcgtc cgcatggacg gtccacggcg cctcggagga ttttggcgat
     9361 tccgccggcg ccggcgatcc gccggcgaag cgcgccaggt tgatgccgcc gtcgggctcg
     9421 cgcacaacct cgatgcgcgc gccgcgcacg tccacgcgcc gcacgccgac gtcgcgccgc
     9481 gagagcgaga acgcgacatc gtgcacgtcg atctccggaa gcgcgatcgg cgcgctcgcc
     9541 gccacgccgc gctcggcgag caccaggtcg cgcacgccga tcgacggtag cgcgacctcg
     9601 agcgacagcg ggttaagctc gaacttgtag ctgccgcgga actgcagcga gcccgatacc
     9661 atctcggcgg gcagcttgtc ctcgaggtag tcgtcgagcg ccgcaagccg caggtcggcc
     9721 acgctgaacg tgccgcgcga gccgaggggc tggaccgtga acgccccgct ccactccagt
     9781 ctcgctccga tccggctcga ggtcgtaaag ctgtactcgt tgctgtggcc gacgtcggtg
     9841 cggaagtctc tcagcgaaaa gcggatcggc gtgaaggcga cgttgaacgg ctgggcgagc
     9901 gagcggtcct ggacgccgat acggccatcc tcgaccgaga acctgccgat gtgcacgcgc
     9961 agcggcgcct cgtcggtctt cggcttgtcc tgcgccggcg cggccggcgg cgcgaggcgc
    10021 gccaggttca ggctgccatc gggcgccacg atcacttcta tgtccggcgc atcgagatcg
    10081 atctccttga agaccacccc gcgccgccac agcgacgaga tctcggcgtt gacgtagagg
    10141 ctccggaacg agacgagcgg cgcgccatcg gcctcggtga gcttgaggtc gatgagggtc
    10201 gcggcgagcg tgaacggatt gaagcggatt tcgccgatcg caatccggcg ctgcagcgtc
    10261 tcggcgacgg aggcttcgat ctgcgagcgc gcgatgtacg gcacgaggaa gaagcccgcc
    10321 gccgcatagg cggcgagcag cagcgccgac acgccgagca gaatccggcg agcgcgcagg
    10381 cgtgcgacaa gcttgttcat tagcctccca tccgcattga atcagcggga cgcggcaaac
    10441 ggcattggac catagtccaa tacgccgggg cgctgcgctt tttttaggcc cgctcggggt
    10501 tgcccgcgcc cgccatcagg ttggcgtcgc gggcgagctg ccaggtgccg gcagcgttct
    10561 tgcggaaaac ggtcaggacg tggcccgaac gctcggtacg cgcgccgtcg cgcttggcgg
    10621 tgagcacgac agtcaggtgc gaccacgcgt aagccaggtc gtcgcagatt ccgaggtcct
    10681 tgagctgctg cctcggctcg atgcgcgcct tggccgaaat cctgcgaaac ccctcggcga
    10741 acgcggtctt ggtgatcggc ggattgcccg gcgtcagaaa caccgcgtgg tccgcggtca
    10801 acgagaggag cccctcgacg tctccttcgg ccgtacggcg ccgccactct tccatgattt
    10861 gcctgatctg ctgctcgtcg gatgccattc cactgacgcg agccagaaac gtgcccgcag
    10921 gaaattcggg gacggagccc gaaccaatta gggctccgtc cccgaattac gggccgaagt
    10981 accagccgcc gttcacgtcg atggtggcgc cggaaacgta gccgccggcc tccgagcaca
    11041 gcatcgccac tactccggcg atgtcgtccg gctttccggc cctcttcaga ggaagcgact
    11101 cgatgaaggc ggcgccggca tctccccagc ccgcggcctc gatgcgcggc gtcttgatga
    11161 agcccggggc gatcgcgttc accgtgatgc cgtgcggccc gagctcgagc gcgagcgtct
    11221 tggtgagccc gatgattccg cctttcgccg ccgcatagtg cacgccaacc cggtcgctgc
    11281 cgcggcgcgc cgcgatcgag gcgaagttca cgatgcggcc gaacttgcgc gccttcatgc
    11341 gcggcgcgaa ctcgcggcac acgaggaacg cggtagtcag atttgcggcg atgacggcgc
    11401 tccagtcttt ctcttccaga tcctcgagtg gaatcgcacc gggaatgggc ggcagcgtgt
    11461 ccccgcgcgt cggcgccgcg ggtccgccgg caatgttgat caggaaatcg ggcgtgccga
    11521 cttcggcaga cagcttctcg atgtcgtccg catccgtgag atccgcgacc agtaggttgg
    11581 gggcggggtg aatatccgtt gcaaagactt ccaccccctc acccgcaagc cttttcgcaa
    11641 ccgcgtcccc gatcccgctc gccgcgccgg tgacgagcgc cttgcgaccc ttcaatcctt
    11701 ggagcggcac gcgagtagcc tacaccggct cacttcgact tggcggcaga ctcctccagc
    11761 gtcttcttgc acaggtcctg ggttccgcgc acgcggtcca gcatcccctg cgcaaacacc
    11821 gatatcccgc cggtcgccac ggcggcggcg cccgaggtcg cgcccttcgc accgaccccg
    11881 agcttagggc tcgcgagcgt gccggccagc tcgatgaacg ggttggcgaa cattccggcg
    11941 gtgatgccga cgccggtgcg cggccgggtg ttgaagtcga acctgagcgc ttcggtgccg
    12001 aggtcgacct tgccgccggc gacgatcgtg accttcacgg attgcatcag cacgggcttg
    12061 accgccacct ggccgtccag gatatcggcc cggaccaccg tgcattcgag ctgcgtgtaa
    12121 gggtcctgcg ccgagaacgg attcaacttg ccggcgagct cgcgaagcag atcgccgccg
    12181 atcaagccta cgacaccggt cgagagcttg cccgccccct gggtcaccac gacccggcca
    12241 ttggcgcccg ccgccatctg ccgcgccgag gcgcctttca ccagcaggct cgcttccaca
    12301 ctggtcggcg gcgcatcctt ggggtcgatc gcgtctccgg cgccgagact aaagcgcaca
    12361 ttcttcgcgg acacgctgat gctgagcgcg ccgctgccgt cgtctgcggg cgtgagcttg
    12421 accgcgctcc ggatcgtgcc gccgaagttg tctgtcgcgc ggccttccag ggtcagccgg
    12481 ccaccctcga ggagcagcgt gctttcgacg tcccgcagga tctccgtacc cagcctgact
    12541 tcggccgagc tgaaacgcag cttggcgtcg gccagcttca gcttgtcgag cggcaacggc
    12601 gcctcgtcga agacgtattt gcgcgccgct tccttcggct tgggtttcgg tttcgccttc
    12661 gagtcgggca cgagcggcgt caagtccagg cggggcgaag caagctcgat gtccacgcgc
    12721 ttcttcttgg agccggccat cgaagcgcgc gcggaaaact cgctctcgcc gatttttccc
    12781 ttgaggctct tcacttcgag cttgtcgcga ctgcccgcgt aggtgccgct catggataac
    12841 ggcgtctccg gcagtcccgc gcgcaacttg gccaggcttt cggcggcgag ctcgaagcgc
    12901 aggtcggcgc tcccgcgggc cgggcggagc gttccgtcga cgcgtgctct tgcgccggcg
    12961 aatctcgcat tcatgccatc gaggttgatt tccgcgcgcg aggcggtcag gcggccgccg
    13021 aactcgaggg cgccggccgg caggccggcc acgtcgagcg ccgccgccag gcgctcgaga
    13081 ttcgccaccg agcccttgac gcgcagatcc gagccgggca ggccaagagc gcgcagcgtg
    13141 ccctctacgc tgagcgtaat gtcggcgagc ttggcggcaa cgtcgagacg ggtaaggtcg
    13201 ccagcgtcgg cgagcttcac ttcggcgacc aaggcgccgc ttgcgatcac cggcaggtcg
    13261 agcttcttga gcatcgcgcc gatgtccggg tgcccgagct tcacggcgag atcggctccg
    13321 tccaggggat cgaagcgacc gattccgcct tttacgtcga gccgcagatc cccgaccccg
    13381 gcctcgatcg cgatgcggat gtcgcgcccg gaaaagagcg catcgagggg ccccagctcg
    13441 ccgctcacgc tggcggggaa ttcgtccagg ctgcctttcc cggagatcgc gagcaggccg
    13501 gcggtgcctg ggccgatggc gaacgtttcg acgaacgcca cacggtcggg cttgccgcgc
    13561 tcgcgatagg tgatccgcac attggcgagc ttgccgttct ggatgacggg aggcagcgcg
    13621 ggagcgctcg cgccgggctc ggccgcggcc tcctcgggcg cgctcgcagc gccgagaacc
    13681 cagttgccct ggccttcggg cccggtttcg agaaggacgg acaggtcgcg cacctcgaac
    13741 gagcgaacgt cgaccggacc cgagaacaac gaccacagcc cgatccgcac cgacacgcgc
    13801 ccgagctcga tcatctgggg ctgcgagccc cagtccgcgt ttgccaggcg cacgcgctcg
    13861 gcgaccacgg agatcgacgg cagcacctgc agttcgagcg cgccgtcgat cgcgaaggac
    13921 ctgccggtct gctgcgcgac gagggcctcg atgcggccct tgtgccggct caggtcgcca
    13981 aaggcgaggt acagcacggc cgcggcgggc accgccacga tggccgagag gaaaatggct
    14041 gcgaggcggc gaagattacg cggcatacga ggctccgttg gcgtcgaccg tgaccccggt
    14101 catgtacgaa gtgtcgtcgg tgcacagggc aacgatgagc gcggcgcact cgtccacggt
    14161 ccccacgcgc cccatgggaa tcgtcttcag cgagccttcc ggcccgtgat cctgtttcgt
    14221 aattccaagc accttcttca gccggccgct ggcgatcacg ccgggcgcaa cgcagttcac
    14281 ccgcacgccg aacggcccga gctcgagcgc gagctggcgc gtgagcccca tgacgcccgc
    14341 cttcgcaacc gcgtacgggc cgctggagcc ctcggagccc ggcgggatgc ctttgcgcgc
    14401 cgcgaccgag gcgacattga cgatcgcccc gccctgcttc ttcaggtgcg gcgcggccgc
    14461 gcgcgcgcaa tagaacaccg agtcgaggtt ggccgcgacg atggtgcgcc agtcctccag
    14521 cgagcaattg gtgaggcctc gaaagccgag cggcggcgcg gcgaccaggt ccttgcgcgt
    14581 aatgccccag cttccgccga cgcagttgac cagcacgtcg atgcggccga agcgctttac
    14641 cgctttttca atcatttcct gtactgaaga agggtccgaa acgtcgcagc gcacgcagaa
    14701 gcattcgcgc aaggcggaaa agccttcctc gtaaagatcc gccacgacga cctgcatgcc
    14761 ctctttcagc agccgccgcg cgacctcgtg cccgatgctc tcgggcttgg cggcgccggt
    14821 gacgacagcg accttcattt gagacgcgat gagcgcattt caaccgcgta ctacgcggtt
    14881 ccttaatacg ccgatgccct cgacttcgag ctcgacgacg tcgccgggtt tgaggaactt
    14941 catttgctcc aggccacagc cgttgccgac ggtgcccgag ccgaagaatt cgccggggtg
    15001 cagggtctcg gagcgcgagg cgtgcgcgat gcagtcctcg aacttccagt gcatgtcgcg
    15061 cgagttgccg cggccccatt cctcgccgtt cacccgcacc gccatctcga gtcggtaggg
    15121 gtcgccgagc tcgtcggcgg tgaccaggca cggtcccatg gcgttggcat tgtcgaaatc
    15181 cttgcctttg cccggcccga gctggccggc catctccttc gcctgctcgt cgcgcgccga
    15241 gaagtcgttg aaaatggtgt agccgaagat gtggctgcgc gcctgctccc tgggaatgtc
    15301 cttgcccggt cttccgatgt agcagccgaa ctcgagctcg aagtccagcc gctcgctgta
    15361 cgccggccag ggcacctcgg cttccgggcc gcagaccgag aagcggctag ggtggtagaa
    15421 gatcgggcgc tcgtaccagg cttgcggggg actcttccca atcgcgcgga aggcctgcac
    15481 caagtgctct tcgaaacaaa ggaaatcgcg catctggggc ggatggggaa tcggcgcgag
    15541 cagcgtgacg ttgcccacct ccagcacggt gggcgagcgc ttgatcgatt cgtaggcgcg
    15601 gtcgagggcg ttctggccgc cttcgacgat ctcgagcacg ctcgcgaacc ccggcagcgc
    15661 cgcgaccttc ctgccgccgt cgatcagggc gcccggcacc ggagcgcgcc cttccctccc
    15721 cgctgcgagc cgaaacgtca cgagtttcac gctgcgtttg ataatacact tcgctctatt
    15781 cgaggaggga tcatgcgcca gttcaacgaa gacacgctga ccaacgaagt cgtcgcgcgg
    15841 ctgaagaaga ccaagaaccc gcgcctgcgc gaaatcatga ccagcctggt gaagcacgtg
    15901 cacgccttcg cgcgcgaagt gcatctcacc gaggaagaat ggttcgaggg catcaagttc
    15961 ctcacctcgg tcggccagaa gtgcgacggc aagcgccagg agttcatcct gctctccgac
    16021 gtgctcggcc tgtcgatgat ggtggtcgcg ctcaaccaca agacccaggc cggcgccacc
    16081 gaggccacgg tgctcgggcc gttcttcgcg cacggcgcga aggagttcgg ctacggcgcc
    16141 gacctgcgcg aaggcgcgac gatgaagggc gaggacgttt gggtgagcgg cagagtgctg
    16201 tcggtggacg gcaagccgat ccccaatgcc gcgctcgaca tctggcaggc caaggccgac
    16261 ggcatctacg atctgcagac cgagggcgaa ttcgagctgc gcggccgcgt caaggccaac
    16321 gccaagggcg agttcgcgtt cgcgagctac aagccgaact tctacagcat cccgatcgac
    16381 ggtccggtcg gcgacatggt ccgcgccacg accgggaacc acatgcgccc ggcgcacatg
    16441 cacgcgatcg tctcggcgcc cggctaccag caggtgatca cccacgtgtt cgtcgagggc
    16501 gatccgcatc tcgacggcga cgcggtgtac gcggtcaaga actccctcat cggcaagtac
    16561 aagaaagtga gcaaccccga gcaggcgaag aagcgcggcc tgccgaaccc gttcctcacg
    16621 ctgacctggg atttccggct cgcggccgac aaggcgggcg cgaagaaagc ccgcaagaag
    16681 atcgccgcca gcgacgccgt cgcaacatga tctggcgcgt tcgccgggtc ctcaccggcc
    16741 acgacgccga gggtcgctcg accttcatcg ccgacggcga ggcgccgaac gtgaaggaga
    16801 tgaccgcgat caccgggctc gccctcaccg acctgtggga aaccggcgcg gctccggcga
    16861 gcaacctggg cctcaaggac gcggccgacc ggccggtcca cctcgagccg ccgaagaacg
    16921 ggacgatcct gcgcatcgtc gagtttccgc ccgactcggc atggcgcggc gcgaccgacg
    16981 gaagcgcggg attcaaggcg atcggcgccg ggcacgcgca ggacaaatcg tcgagcgacc
    17041 cgatgatgca caagacggcg acggtcgact acatcatcgt cctcaagggc gagatctacg
    17101 cgatcatgga cgccggcgag aagctcctgc gcgccggcga cgtgctcgta cagcgcggca
    17161 ccaaccattc gtggagcgtg cgcagcaacg agccctgcat catcgccgcg gtgctggtga
    17221 gcgcgaagcc cgtgagtgcg ccttcgacaa agaagccttc gaagaagtcc aagaagaaaa
    17281 agaaatgagc tttggttctt gcccccctga caaggggggt ggcgcgaagc gccggggggg
    17341 ttgcgatgcg tagaaaacca accccccgct cgcctgcggc tcacgacccc cttgtcaggg
    17401 ggtcaaagca agcagtgggc gtcatcgggc tgggcatcat gggctcggcc atgtccgcga
    17461 atctcctgaa ggccggcttc gacgtgcgcg gctacgacat cgtggcctcg cgccgtgccg
    17521 cgctgaaacg tgccggcggc aagcccgctt cgtccctctc caggctcaac gcggaagtcc
    17581 tcatcacctc cctgcccagc gcagaagcgc tgcatgccgt ctcgcagcaa ctgaagagaa
    17641 agtgcatcgt tgtcgagaca agcacgcttc cgatcgaaga caaggtcaag gcgcgcgatg
    17701 cgctcaagcg caagggcatc acgctcctcg actgcccgct ctccggcacc ggggcgcagg
    17761 cgaagaccgg ggacctggtg gtcttcgcga gcggagatcg caaagcgttc acgaaagcga
    17821 aatcgatctt tcccggcttc tcgcgagcgc accactacct cggcgaattc ggcaacggca
    17881 gcaagatgaa gtacgtcgcg aacctgatgg tggcgatcca caacgtgtcg gccgccgagg
    17941 cgttcgtgct cggcatgaag gctgggctct cgcccaaggc gatctacgag gtgcaggcga
    18001 acggcgccgg cacttcgcgc atgttccagg tgcgcgggcc gatgatggtc gccgggcgct
    18061 acggcgacgt gacgatgaag aacgaggtct ggcagaagga catgaagatc atcggcgagt
    18121 tcgcggccag gctcggcgtg ccgacgccgc tgttcaacgc ctccgcggcg atctacagcg
    18181 cggcgatggc ccagggcttc ggcaagcagg acaccggcgc ggtgtgcgcc gtgctcgagt
    18241 cgatggcgaa gcaccgccgc taattcgggg tcagggtgac aattcgtttt tgcgtacgcg
    18301 aaaactgaat tgtcaccctg accccgaatt actgcagcga gcgcagcgta gcgagcagcg
    18361 tatcgatgtc gaaaggcttc tgcagcgtgg cgtccacgcc gatcagctcg gcatccggca
    18421 ggtagtcgcg cttggtgaac tgggcgccgc cggacaccac gatgatcctg gtccgcggga
    18481 actcgtggcg gaaggcgtcg atcgcctcga agccgtcggc gtcgggcatg aagatatcgg
    18541 tgatcagcac gtcggccggc tgcccgcgct gcagcgcgag cgcttcgcgc gcgttcgccg
    18601 ccacgtcgac cgcataaccg gcgatctcga gcgcgagctt gagcgtgagc cgcatgtcgg
    18661 cgtcgtcgtc ggcgaccagc agccgcttgc ttctcgcttc ggtcatcgca ggcccgccat
    18721 cacctgcttc agccgctcgg gcgcaaccgg cttcgtcagg tgggcgtcga agcccgcctc
    18781 gtacgcgcgg cgctggtcct cgctctggcc gtagccggtg atcgcgatca ggccgatgcg
    18841 ccggccgccc ggcgcggcgc gcagccggcg ggcgacttcg tagccgtcca ggtcgggcag
    18901 cccgatgtcg atcagcgcca cgtccgggcg cgcctcggcc gccagcgcga gtcccgaagc
    18961 gccgtcgcgc gcttcacgca cctcatggcc gctgaaggcg agcgtttcgt gcagcatctg
    19021 gcgcgtgtcg tcgttatcct cgacgatcag cacgcggcgc ggttgcatgc gcggcgcgat
    19081 ttccagcgcc gccggcgccg ccgcggcgga gggcgccagc accgcgggta gcctcaccgt
    19141 gaaggtcgcg ccctgccccg gacccgcgct ctcgccggcc acgctcccgc cgtgcatctc
    19201 ggtgagccgc ttcaccagcg cgagcccgac gccgaggccg ccggcggcgc ggtccagccc
    19261 gcgctcgccc tgcacgaaga ggtcgaacat gcgctcggcc gagccgggcg cgaggccctc
    19321 gccttcgtcg ctcacgcaaa gctgcgccca ttccccctcc gggcccacgc gcaccgcgat
    19381 gcgccggccg gcgggggtga acttgaccgc gttgtcgagc aggttggaca ggacctgctc
    19441 gatgcgcgcc cggtcggcgt cgacccacgc cgctttcatc tccacggaaa cgtggtggcg
    19501 ctcgagccgc cccgaggcgc gccagacgtt gagcaggttg cccgcggcct cggcgaggtt
    19561 gaagcgctcg cgctcgagcg cgaccttgcc catcgcgacg cggctgatgt cgagcaggtc
    19621 gccgacgagc cgcgccatgt gcttggtctg ccgctcgacc accgcgcgcg ccttggcggc
    19681 ggcctggtcg ccgggctgcg cgaacttcag cacgtgcgcc gccgcggtca gcgcggcgag
    19741 cgggttgcgc agctcgtggc tgagcatggc gagaaactcg tccttggagc ggtcggccgc
    19801 ctgcagcgcg gttttctcgc gctcctgcag ctcgctgcgc tcgcgtaccg ccgcggcagc
    19861 ggcgcgcagc gcctcgtaga gccgcgagat ctcgtcgatc cttcccgggt ccgccacccg
    19921 gacgtccttg ccctgcccca tggcctcggt ggccgcggcg agcgcggcga tgggcgaagc
    19981 gatgcgccgc gccatgtacc agcccagcag cagcgcgagc gcgagcacgg tcgcgatgcc
    20041 tgcagcaaat ccgaagaacg ttcgggtggc ggcggcctcg accgagcgcg ccgggatggc
    20101 gatgccgagc acccagccgc tcagctcgga ggtgacgtag ggcgtgtagg tccgggttcc
    20161 ctccagcgtc gagcccggga accagccgtt cggcgagcgc tcgatcgcgg cgcggaagct
    20221 ctccgacacc gctcgccccg gcgccatcgg cgggaggcgg gcgatgaagc gccggttgcg
    20281 atccaccagc gcgatcaccc agctttccgg aagccgctgc gcgcgcagca cgtcgg
//
LOCUS       DATBSZ010000250         5990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1139897, whole genome shotgun sequence.
ACCESSION   DATBSZ010000250 DATBSZ010000000
VERSION     DATBSZ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139897"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..703)
                     /locus_tag="VIG70_16015"
     CDS             complement(53..703)
                     /locus_tag="VIG70_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252105.1"
                     /translation="MRGFVLAAVLLAFPAVADEIAEARKRWESSPHGPMLERILPPTF
                     ELRQLPQRDSPGAKLVLAYCVQCHNLPNPAMHHAAKWPGIVERMVLRMEGKGNMGTLM
                     AEMMAGVKAPGAEDTKAIVAYLRRHAQKPLDPARYPELNRSSGEAFRLACNQCHVLPD
                     PQRYTAAQWPRVVARMQENMEWMNRVVGSKPAPDEPQLRVGEINAFLAKHARKPGS"
     gene            complement(706..1734)
                     /locus_tag="VIG70_16020"
     CDS             complement(706..1734)
                     /locus_tag="VIG70_16020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8252106.1"
                     /translation="MLADRIRAEIAAQGGWTSFARYMELALQAYYGRSDRQFGAQGDF
                     ITAPELGSLFGRTLARQLRELEGPILELGAGSGALASALCRELERDYLILETSGALRE
                     RQAARLGGRVKFLDHLPEGFRGVVIANEVVDAMPVHAVGWRPAGIVERGVSTQLTWEE
                     RPASGSVLDAARAIEVPVPYDSEIGLAGQAWMRSLAERLEAGAIFVIDYGFSRREYYH
                     PQRSMGTLMCHARHRAHGDPFERPGEQDITAHVDFSALARAARESGLEALGYATQAQF
                     LVNCGITDVLGQANLENALHYAPIAAEAQQLLSPAEMGELFKVLAVGRGVKQPLLGFS
                     RGDRSNTL"
     gene            1767..2513
                     /locus_tag="VIG70_16025"
     CDS             1767..2513
                     /locus_tag="VIG70_16025"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HEY8252107.1"
                     /translation="MDDSLEGKTALVTGGGRRVGAAIARRLHAAGARVVLHYRDSEAD
                     AARLESELNAVRARSAAKVKAELLAPVAPRALVAAALESFGRLDILVNNASTFFPVPV
                     GEMESSHWEEMIGANLRAPLFIAQQAAPELAKNEGSIVNIVDIHAERPLKGYALYTIT
                     KAGLAAMTRSLALELAPHVRVNGVAPGAIAWPEDGQFEDAERARILATTPLGRVGSPE
                     DVARAVHFLACAPYVTGQILAVDGGRSIFI"
     gene            2523..2843
                     /gene="sugE"
                     /locus_tag="VIG70_16030"
     CDS             2523..2843
                     /gene="sugE"
                     /locus_tag="VIG70_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEY8252108.1"
                     /translation="MPWLWLTLAGLLEIVWAIGLKYTDGFTRPVPSAITIAAMIASVY
                     FLALAVRTIPIGTGYAVWTGIGAVGVAILGMVLFDEPRTLLRIASILLIVAGITGLKL
                     VTPA"
     gene            2840..3637
                     /gene="ttcA"
                     /locus_tag="VIG70_16035"
                     /pseudo
     CDS             2840..3637
                     /gene="ttcA"
                     /locus_tag="VIG70_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
     gene            3741..4484
                     /locus_tag="VIG70_16040"
     CDS             3741..4484
                     /locus_tag="VIG70_16040"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEY8252109.1"
                     /translation="MTRSVVRLIGRSSLVGLLALAGASVTAENPDNVAVDPAAVSEPV
                     VQVWGARTRGAKGIFGVHSWIAVKPAGESEYTVYEVIGWRLRWADTAVVVRNRRPNHW
                     FGAPGELYAEKRGAGVDELIRRIDQAAREYPYANTYTVWPGPNSNTFTAWVARAVPEL
                     EVDLPATAIGKDYIGDSMISTAPSGKGFQFSLRGLFGVAASGVDGFELNILSLNFGVS
                     ASGIKLPIVGRIGLPRRPDALAIEEPASP"
     gene            complement(4481..>5990)
                     /locus_tag="VIG70_16045"
     CDS             complement(4481..>5990)
                     /locus_tag="VIG70_16045"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEY8252110.1"
                     /translation="MVTGRPQAMMVHVGVGTANSLNGLINASRQNVPILFTAGRTPIT
                     ESGALTAARNNYIHWAQEHFDQGGMLREFVKWDYELRHAEQVETVIDRALAIARSEPQ
                     GPVYVTLPREILASSFQSQYSEASTIAPASPPAADPEALEEAAKLLGAAQHPLLITAS
                     GGRTQESSRAIEQLAQTMAVPVVHYRPRHLALSTEHPMHCGWDPHALLKEADVVLVVE
                     CDVPWIPKEGKPKPEAKVIHIGPDPLFASYPLRGFRADIALTGAVAPTLQALWRAAQK
                     QAVSVKQIEERRKTVSLVSEDIRKKARGGVDPAPASITGKWLSACINKVLDANTILVN
                     EYPTVLEEMVIREPGRYFGNPSAGGLGWGMGAALGAKLASPDKTVVCAVGDGAYMFGN
                     PTAVHFVSEAMRLPVLFVIANNARWAAVHRSALSTYPTGVASKEKQPPFATLEPSPRF
                     EHVVKASGGHGERVTEPQQLLPALEKALKIVKEEKRQAVLNVCLEASYVKTS"
BASE COUNT          908 a         2073 c         2001 g         1008 t
ORIGIN      
        1 aggatgccgc tgcgctcggt gaagcggtcg atcgcgccgg cgatccgcag catcagctac
       61 ccggttttcg cgcgtgcttc gccaggaagg cgttgatttc cccgacgcgc agctgcggct
      121 cgtcgggcgc cggcttcgat ccgaccacgc ggttcatcca ctccatgttc tcctgcatgc
      181 gcgccaccac ccgcggccat tgcgccgcgg tatagcgctg cgggtcgggc aacacgtggc
      241 actggttgca cgccaggcgg aacgcttcgc ccgaggaccg attgagctcc ggatagcgcg
      301 ccgggtcgag cggcttctgc gcatggcgtc gcaggtaggc gacgatcgcc ttggtgtctt
      361 cggcgcccgg cgccttcacc ccggccatca tctcggccat cagcgttccc atgtttccct
      421 tgccctccat gcgcagcacc atgcgctcga cgatgccggg ccacttggcg gcgtggtgca
      481 tcgccggatt gggaaggtta tggcactgga cgcaataggc gagcaccagc ttggcgccag
      541 gcgagtcgcg ctgcggcaac tgtcgcagtt cgaatgtggg cggcaggatg cgctcgagca
      601 tcgggccgtg cgggctggat tcccagcgct tcctggcttc ggcgatctcg tccgcaaccg
      661 ccgggaaagc aagcagtaca gccgcgagaa cgaacccacg catgcctaaa gcgtattgga
      721 tcggtcgccg cgtgaaaacc ccaggagcgg ctgttttacc ccgcgcccga ccgcgagcac
      781 cttgaagagc tcgcccatct ccgcggggga aaggagctgc tgcgcctcgg cggcgatcgg
      841 cgcgtagtgg agcgcgttct cgagattcgc ctgccccagc acgtcggtga tcccgcagtt
      901 gacgaggaac tgcgcctgcg tcgcgtagcc gagcgcctcg aggcccgact cccgcgcggc
      961 gcgcgccagc gcgctgaagt cgacgtgcgc ggtgatgtcc tgctcgcccg gccgctcgaa
     1021 cggatcgccg tgcgcgcggt ggcgcgcatg gcacatcagc gtgcccatcg agcgctgcgg
     1081 atggtagtac tcgcggcgcg aaaagccgta gtcgatgacg aaaatcgcgc ccgcttcgag
     1141 ccgctcggcg aggctgcgca tccaggcctg gccggcgagg ccgatctcgc tgtcgtacgg
     1201 aaccgggacc tcgatcgccc ttgccgcgtc gagcacactg ccgctcgcag ggcgttcttc
     1261 ccaggtcagc tgtgtcgaaa caccccgttc aacgatgccc gccggccgcc accccaccgc
     1321 gtgcaccggc atggcgtcga cgacttcgtt cgcgatcacg acgcctcgaa agccctccgg
     1381 aaggtgatcg aggaatttca ctctgccgcc gagccgcgcc gcctggcgct cgcgcagcgc
     1441 gccgctcgtc tcgaggatca ggtagtcccg ctcgagctcc ctgcagagag cactggcaag
     1501 cgctccgctc cccgcgccga gctcgagaat cgggccctcg agctccctga gctgccgggc
     1561 cagcgttcgt ccgaacaggc tgccgagctc cggcgcggtg atgaagtctc cctgcgcgcc
     1621 gaactgccgg tcgctgcgtc cgtagtaggc ttgcagcgcg agctccatat agcgcgcgaa
     1681 ggaggtccag ccgccttgcg ccgcgatctc cgcccggatt ctgtcggcga gcacgcgaag
     1741 aatctaccct agaattcaag gcgttcatgg atgactcgct cgagggcaag accgcattgg
     1801 tgacgggcgg cgggcggcgc gtcggcgcgg cgatcgcgcg gcgcctgcac gccgcgggcg
     1861 ccagggtggt gcttcactac cgcgattccg aggccgacgc cgcgcggctc gaatccgagc
     1921 tgaacgcggt gcgcgcgcgc agcgcggcga aggtgaaggc cgagctgctc gcgccggtgg
     1981 cgccgcgcgc gctggtcgcc gccgcgctcg agagcttcgg ccggctcgac atcctggtga
     2041 acaacgcttc gaccttcttc cccgtcccag tcggcgagat ggagtcttcg cactgggagg
     2101 agatgatcgg cgcgaacctg cgcgcgccgc tcttcatcgc gcagcaggcg gcgccggagc
     2161 tggcgaagaa cgaaggctcg atcgtgaaca tcgtcgacat ccacgcggag cggccgctga
     2221 aaggctatgc gctctacaca atcaccaaag ccgggcttgc cgcgatgacc cgatcgctcg
     2281 cgctcgagct cgcgccgcac gtgcgggtga acggcgtcgc gccgggcgcg atcgcgtggc
     2341 ccgaggacgg ccagttcgag gatgcggagc gcgcgcgcat cctcgccacc acccccctcg
     2401 gccgcgtcgg ctctcccgag gacgtggcgc gcgcggtgca tttcctcgcc tgcgcgccct
     2461 atgtcaccgg ccagatcctg gcggtcgacg gcggccgctc catcttcatc taggaagcgc
     2521 ttatgccctg gctctggctc acgctcgccg gcctgctcga aatcgtctgg gcgatcggcc
     2581 tcaagtacac cgacggcttc acccggccgg tcccctccgc catcaccatc gccgcgatga
     2641 tcgccagcgt gtactttctc gcgctcgcgg tgcgcaccat cccgatcggc accggctacg
     2701 ccgtctggac cggcatcggc gcggtcggcg tcgcgatcct cggcatggta ctgttcgacg
     2761 agccacggac gcttctgcgt atcgcgtcca tccttctcat cgtggccggc attaccggcc
     2821 tgaagctggt gactcccgca tgagagccca acccgtccgc ctgcaccgcg acccgcgcaa
     2881 agccgactac gagctcggca agctcgcgaa gcgcctgcgt cgccaggcag gggaagcgat
     2941 cgccgacttc agcatgatcg aggcgggcga ccgggtgatg gtctgcctct cgggcggcaa
     3001 ggacagccat ggcctgctgg atgttctcct gtccctgcag gagaagtccc ccgtacggtt
     3061 cgagctgatc gccgtgaacc tcgaccagaa gcagcccggg tttccggcgc acgtcctgcc
     3121 cgagtacctc gaatcgcgcg gcgtgccgtt ccgcatcgtc gagcaggaca cctactcggt
     3181 ggtgaagcgc gtgatcccgg aaggaaagac catgtgctcg ctgtgctcgc ggctgcggcg
     3241 cggcgtgctg taccgggtgg cgggcgaggt cggcgcgacc aagatcgcgc tggggcatca
     3301 ccgcgacgat attcttgcaa catttttcct aaacctgttc catggcggcc agctgaaggc
     3361 gatgcccgcc aagctcgcgt cggacgacgg ccggcacgtg gtgatccggc cgctcgccta
     3421 cgtcgccgag tccgacctcg cggcgtatgc cgacgccaag ggctttccga tcattccctg
     3481 caccctgtgc ggctcgcagg acaacctgca gcgcaagacc ataggcgaga tgctgcgcga
     3541 ctgggagcgc cgccaccccg gccggaccga gtcgatggca cgtgccctgg ccgaggtgcg
     3601 gccctcgcac ctcctcgacc gcaagctatt cgatttcaag aatttgccgt aatggctctt
     3661 tcgtcttatt tcgggcgcag acgacatggt tcatcctgtc atcgtgcgcg gcctacaatg
     3721 cgttgagtat ccaagaaacc atgactcgct cagttgtcag gctcatcggc cgctccagcc
     3781 tcgtcgggct ccttgccctg gcgggcgctt cggtcacggc cgagaacccg gacaacgttg
     3841 cagtggatcc ggcggctgtg agtgagccgg tcgtgcaggt ctggggcgcg cgcacccgcg
     3901 gcgccaaggg catcttcggc gtgcatagct ggatcgcggt caagcccgcg ggcgaatccg
     3961 agtacaccgt ctacgaggtc atcggctggc gcctgcgttg ggcagacacc gcggtcgtgg
     4021 tccgcaaccg caggccgaac cattggttcg gggctccggg cgagctgtac gccgagaagc
     4081 gcggcgccgg cgtggacgag ctgatccggc gcatcgacca ggccgcccgg gagtatccct
     4141 acgccaacac ctacaccgtc tggccggggc cgaattcgaa cacctttacg gcctgggtcg
     4201 cgcgcgcggt ccccgagctc gaggtcgacc tgcccgccac ggcgatcggc aaggactaca
     4261 tcggcgacag catgatctcc actgcgccga gcggcaaagg cttccagttt tcgctgcgcg
     4321 ggctgttcgg cgtggcggct agcggcgtcg acggcttcga gctcaacatc ctgagcctca
     4381 acttcggcgt gagcgcctcg ggcatcaagc tgccgatcgt cgggcgcatc ggcctgccgc
     4441 gtcgcccgga cgccctcgcc atcgaagagc cggccagccc ctagctggtc ttgacgtagc
     4501 tcgcttcgag gcagacgttc agcaccgcct gccgcttctc ttccttcacg atcttcaggg
     4561 ccttctcgag cgccggcagc agctgctgcg gctcggtcac gcgctcgccg tggccgccgc
     4621 ttgccttcac cacgtgctcg aagcgcggcg agggctcgag agtggcgaac gggggttgtt
     4681 tttcctttga agcaacaccc gttgggtaag tcgacagcgc ggaccggtgc accgccgccc
     4741 agcgcgcgtt gttcgcgatc acgaacagca ccggcaggcg cattgcttcc gacacgaagt
     4801 gcaccgcggt ggggttgccg aacatgtagg cgccgtcgcc gaccgcgcaa accacggtct
     4861 tgtcgggcga ggcgagcttg gcgccgagcg ccgcgcccat cccccagccc aggcctcccg
     4921 cggaggggtt gccgaagtag cggcccggct cgcggatcac catttcctcg agcaccgtcg
     4981 ggtattcgtt gacgaggatg gtgttggcgt cgagcacctt gttgatgcaa gccgagagcc
     5041 acttgccggt aatggacgcg ggcgccggat cgaccccgcc gcgggccttt ttccggatgt
     5101 cttccgaaac cagggagacg gttttccgcc gttcctcgat ctgcttgacc gagaccgcct
     5161 gtttctgcgc ggcgcgccag agcgcctgca gcgtgggcgc tacggccccg gtaagagcga
     5221 tgtcggcgcg gaacccgcgc aacgggtagg aggcgaagag cgggtcgggc ccgatgtgga
     5281 tcaccttggc ttcgggcttg ggcttgcctt ccttggggat ccagggcacg tcgcactcga
     5341 ccaccagcac cacgtcggct tccttgagaa gcgcgtgcgg atcccagccg cagtgcatcg
     5401 ggtgctcggt ggagagcgcg aggtggcgcg gacggtagtg gactaccggc accgccatcg
     5461 tctgcgcaag ctgctcgatc gcgcgcgagg actcctgcgt gcgtccgccg ctcgccgtga
     5521 tgagcagcgg gtgctgcgcc gcaccgagca gcttcgcggc ttcctcgagc gcctcgggat
     5581 ccgccgcggg cggggatgcg ggagcgatcg tcgaggcttc tgaatattga gactgaaaac
     5641 tcgaagcgag aatctcgcga ggcagcgtca catagacggg tccctgcggc tcgctcctgg
     5701 cgatggcgag cgcgcggtcg atcaccgtct ccacctgctc ggcgtggcgc agctcgtagt
     5761 cccacttcac gaactcgcgc agcatgccgc cctggtcgaa gtgctcctgc gcccagtgga
     5821 tgtagttgtt gcgcgccgcg gtcagcgcac cggactcggt gatcggcgtg cgcccggcgg
     5881 tgaacaggat cggcacgttc tgccgcgagg cgttgatcag gccgttgagc gagttcgcgg
     5941 tgccgacgcc gacgtgcacc atcatcgcct gcggccgccc ggtcaccatg
//
LOCUS       DATBSZ010000251         3283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_183179, whole genome shotgun sequence.
ACCESSION   DATBSZ010000251 DATBSZ010000000
VERSION     DATBSZ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183179"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VIG70_16050"
     CDS             complement(<1..473)
                     /locus_tag="VIG70_16050"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HEY8252111.1"
                     /translation="MALVAGNVLAVAIAAFYPPITMSIMFLYWAECVVVGAFNVAKMQ
                     YVPGHDRADRIGVAVLFLVHYGGLTFMCFGVLHQLARFELRQRGMPQFDFGAYYAGFI
                     WVVLILAAGHAVSFVRNFLGRREYESRSLDQQMWRPYRRVVLMIGIVFAACCLVIV"
     gene            complement(527..1654)
                     /locus_tag="VIG70_16055"
     CDS             complement(527..1654)
                     /locus_tag="VIG70_16055"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8252112.1"
                     /translation="MAKPAKKIGRYRILGELGRGAMGVVYKADDPNLDRTVALKTISL
                     DKDAEGRADYQKRFMLEAKAAGKLNHANIVTTYDFGEVDGMAYLAMELLEGTDLRKRV
                     QQGPIPPIEAVEIGCQVAEGLAYAHGRGIVHRDIKPANIMLPERGPAKIMDFGLARMR
                     LADHKTSTGIVLGTPRYMSPEQISGQPVDQRSDIFSLGIVLWEMLTGKRLFSGTEMHQ
                     VSHSITHDEHEPPTRVNPDLPAMLDFVVARALKKDPTVRYQDADEMAADLHTCLAELR
                     SRDASADEIAEGSKTLKLGDAEPTVGAPAAAAIVTDTRLPVSRHFDSSAALRRLGKSP
                     KSGRAPRPVGILRRIVRDAPARRLFVAALLGALAGSYVALV"
     gene            complement(1654..2310)
                     /locus_tag="VIG70_16060"
     CDS             complement(1654..2310)
                     /locus_tag="VIG70_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252113.1"
                     /translation="MFLKIQDFARRPATEQARLRAQLQAVVAVTAAEIPPAGRVMLDA
                     ADGAAIVVLDDPRAALRLAERALTAVAVGLPLSAGLNHGALQLAAGRKSGEGMTGDGI
                     AVAASVAAFAGASRILASRTFRDAMAESAPGREAVLAPAGTFNDPGLRAHELFTPNHR
                     ALGRRGRRYSAIAVLLAACLIGTGIGVRSATPSQRPFMEAVLERTEPYWRELVQRVSY
                     "
     regulatory      2422..2516
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            2552..>3283
                     /locus_tag="VIG70_16065"
     CDS             2552..>3283
                     /locus_tag="VIG70_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY8252114.1"
                     /translation="MKIDSKFTDFKVADLALADWGRKEIAIAEWEMPALMAIRQEFAS
                     RQPLKGARIAGSLHMTIQTAVLIETLKALGAEVRWASCNIFSTQDHAAAAIAASGTPV
                     FAYKGESLDEYWEFTHRIFEFGDGKGPNMILDDGGDATLLVHLGPRAAKDGGLLSNPS
                     SEEEVSLFNAIKKKLKTDPDWYSRIGKDIKGVTEETTTGVHRLYQMAKEKRLLFPAIN
                     VNDSVTKSKFDNIYGCRESLVDGIKR"
BASE COUNT          574 a         1088 c         1114 g          507 t
ORIGIN      
        1 acgatgacga ggcagcaggc ggcgaacacg atgccgatca tcagcacgac cctgcgatag
       61 ggacgccaca tctgctggtc caggctgcgg ctctcgtatt cgcgcctgcc gagaaaattc
      121 ctgacgaacg acacggcatg cccggcagcg agaatcagca ccacccagat gaagccggcg
      181 tagtaggcgc cgaagtcgaa ttgcggcatg ccgcgctgac gcagctcgaa cctggcgagc
      241 tggtgcagga cgccgaaaca catgaaggta agcccaccgt agtgcaccaa gaacagcacc
      301 gccacaccga tccgatcggc ccgatcatgg cccggcacgt attgcatctt cgcgacattg
      361 aacgccccga cgaccacgca ttcagcccag tacaggaaca tgatcgacat cgtgatcggg
      421 gggtagaacg ccgcgatcgc cacggcgagc acgttgccgg cgacgagcgc catggcgctc
      481 gggtcttgcg caacccgagc ataaagctcg cgcagcgccc tgcctctcag acgagcgcga
      541 cgtagctgcc ggcgagcgcg ccgagcagcg cggcgacgaa cagacgccgc gccggcgcgt
      601 cgcgcacgat cctgcgcagg atcccgaccg ggcgcggcgc gcggccgctc ttcggcgact
      661 tgcccagccg gcgcagcgcg gcggacgaat cgaagtggcg cgacaccggc agccgggtat
      721 cggtgacgat cgcggcggcg gccggcgcgc cgaccgtcgg ctcggcatcg cccagcttca
      781 gcgtcttgct gccctcggcg atctcgtcgg cgctggcgtc gcgcgaacgc agctcggcga
      841 ggcaggtatg caggtcggcc gccatctcgt cggcatcctg gtagcgcacc gtagggtcct
      901 tcttcagcgc gcgcgccacc acgaagtcga gcatcgccgg caggtccgga ttcacgcgcg
      961 tcggcggctc gtgctcgtcg tgggtgatgc tgtgcgagac ctggtgcatc tccgtgccgg
     1021 agaacaggcg cttgccggtg agcatctccc acagcacgat gccgagcgag aagatgtccg
     1081 agcgctggtc gaccggctgg cccgagatct gttcgggcga catgtagcgc ggcgtgccga
     1141 gcacgatgcc ggtgctcgtc ttgtggtcgg cgaggcgcat gcgcgcgagg ccgaagtcca
     1201 tgatcttcgc cgggccgcgc tcgggcagca tgatgttcgc gggcttgatg tcgcgatgca
     1261 cgatgccgcg gccgtgcgcg taggccaggc cctcggccac ctggcagccg atctcgacgg
     1321 cctcgatcgg cggaatcggg ccctgctgca cgcgcttgcg caggtcggtg ccctctagca
     1381 gctccatcgc gaggtaggcc atgccgtcga cctcgccgaa gtcgtaggtg gtgacgatgt
     1441 tcgcgtggtt gagcttgccg gcggccttcg cctccagcat gaagcgtttc tggtaatccg
     1501 cccgcccctc ggcatccttg tccaggctga tggtcttgag cgcgacggtg cgatcgaggt
     1561 tgggatcgtc ggccttgtag accacgccca tcgcgccgcg gccgagttcg ccgaggatcc
     1621 tgtagcggcc gatcttcttc gccggcttcg ccatcagtag ctgacccttt gcaccagctc
     1681 gcgccagtag ggctcggtac gctcgagcac cgcttccatg aacggccgct ggctcggcgt
     1741 cgcggaacga acgccaattc ccgttcctat gagacaggca gccaaaagca cggcaatcgc
     1801 gctatatcgc cggccgcggc gcccgagcgc gcggtgattg ggagtgaaga gctcgtgcgc
     1861 ccgcagcccg ggatcgttga acgtcccggc tggcgcgagt accgcctctc tgccgggcgc
     1921 ggactcggcc atggcgtcgc ggaaagtgcg cgacgcgaga atgcgcgaag cgcccgcgaa
     1981 cgccgccacg gatgcggcga ccgcgatgcc gtcgccggtc atgccttcgc cgctcttgcg
     2041 gccggcagcg agctgcagcg cgccgtggtt caggcccgcg gaaagcggca ggccgaccgc
     2101 caccgcggtc agcgcgcgct ctgcaaggcg cagcgcggcg cgcgggtcgt cgagcaccac
     2161 gatcgccgcg ccgtcggcgg catcgagcat gacgcggccg gcgggcggaa tctccgccgc
     2221 ggtcaccgcc accactgcct ggagctgggc gcgcaagcgc gcctgctcgg tcgccggacg
     2281 gcgggcgaaa tcctggatct tcaggaacac gacgctggcg tagaaagacg ggctctttga
     2341 ctggctcttt gagggagtct ttgctgcggg catggcggaa ggatgcaggg aatgggcacg
     2401 cttgaacagg gtataatttg ccccgctgag gagcgttgcg ttccccgccc gctcgcaaaa
     2461 tgaggcgacc tgaggcgggg atcaggctca gctaaataca acggcgctcg catcggaaac
     2521 ggtgcgagcg ctttttcgtt ctggagatcc gatgaagatc gattcgaaat tcactgattt
     2581 caaagtagcc gacctcgcgc tcgccgactg ggggcgcaag gagatcgcga tcgccgagtg
     2641 ggaaatgccg gcgctgatgg cgatccgcca ggagttcgcg tcccggcagc cgctcaaggg
     2701 cgcgcgtatc gccggctcgc tgcacatgac gatccagacc gcggtgctga tcgagacctt
     2761 gaaggcgctc ggtgccgaag tgcgctgggc ctcgtgcaac atcttctcca cccaggacca
     2821 cgccgccgcg gccatcgccg ccagcggcac gccggtgttc gcctacaagg gcgaatcgct
     2881 ggacgaatac tgggagttca cccaccggat ctttgagttc ggcgacggca agggcccgaa
     2941 catgatcctg gacgacggcg gcgacgccac gctgctcgtg cacctcgggc cgcgcgcggc
     3001 gaaggatggc ggcctgctgt cgaatccgtc gagcgaggaa gaagtctcgc ttttcaacgc
     3061 gatcaagaag aagctgaaga ccgaccccga ctggtattcc cggatcggca aggacatcaa
     3121 gggcgtgacc gaagagacca cgaccggcgt gcaccgcctc taccagatgg cgaaggagaa
     3181 gcgcctgctg ttcccggcga tcaacgtcaa cgactcggtc accaagtcga agttcgacaa
     3241 catctacggc tgccgcgagt cgctggtcga cggcatcaag cgc
//
LOCUS       DATBSZ010000252         8058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_183335, whole genome shotgun sequence.
ACCESSION   DATBSZ010000252 DATBSZ010000000
VERSION     DATBSZ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183335"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..377)
                     /locus_tag="VIG70_16070"
     CDS             complement(84..377)
                     /locus_tag="VIG70_16070"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY8252115.1"
                     /translation="MPHVVTKSDLVQVAAKAGNVSKVAANEVVNAVFEAIVSNVAKGK
                     RVTLVGFGTFLPRTRKARAGRSPVNGKEIKISAKTIPAFAAGQGFKQAVDKTS"
     gene            517..1542
                     /locus_tag="VIG70_16075"
     CDS             517..1542
                     /locus_tag="VIG70_16075"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8252116.1"
                     /translation="MIRSLTVAFTAALAAALCVPAHAQSTLTYSSWVSPQHHLSVWQA
                     NWTAEVEKATGGRVKFQSLPKHPSAPPGTFDAVRDGLMDLSYVTASYTPARHVMPLMP
                     ELPGAGDTAEVNSVAYSRIHWKHFQKLGEYKGVKLLAVWTHGPGQLFTKKTVAGIGDI
                     QGLKIRTGGGVAEKVANALGASAFVKPAPESYELLKSGVADGVFFPLESIVSFKLDTV
                     LEQATIFPGGMYSSAFGFFMNEDKWNKLAKQDQAIIEKFSYEYAARSNGKSWDAADQK
                     GLDGLKKAGVKIIQADAKLLAEVRKRSAPIIDDWVKQASAKGIDAKAVLAEFRAELKK
                     VAAESKK"
     gene            1689..2087
                     /locus_tag="VIG70_16080"
     CDS             1689..2087
                     /locus_tag="VIG70_16080"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY8252117.1"
                     /translation="MMCLTFVDVVARYVFNRPLRGAFEVTELLLLVLIFGGLPLVSHA
                     DEHVTMDFIDRLLGRGLDLWQRAVHALCAGVMFLLTWLVWIKANRISAYADATDVLRI
                     LYGPFVYFMAVMIALAGLIHLYKAFEWPKD"
     gene            2072..3370
                     /locus_tag="VIG70_16085"
     CDS             2072..3370
                     /locus_tag="VIG70_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY8252118.1"
                     /translation="MAEGLIGLAAMLAIAFLRVPIALAMGIVGIIGYAYMRDWNFTTA
                     FAVAQTKIYETGRNYTLSVVPLFILMGNFVTRAGMSQELFRAAYAFIGHLKGGLAMAT
                     ILACAGFGAICGSSIATAATMAKVAYPSMKRFGYSDRLATGAIASGGTLGIMIPPSTI
                     MVIYGVFTETNIGKLFAAGVLPGILGATLLCLTVRYVTWRDPNAGPPGERSSWRERWA
                     AVRDVWAVAFLFLFVMGGIYGGLFTATEGAGMGAFGAMAFALWRRALNWKTLYAALVE
                     SARTSAMLFMILIGALMFADFINITSMPADLKSFVTRFELSPILVVAAICVIYVLLGT
                     AMEELSMVLLTLPVFFPVIMHLGYDPVWFGILIVVVVEIGLISPPVGMNLFVLKTLLP
                     GVPTTTVFRGVMPFMLADCVRFALLIAFPAISLWLPSFMK"
     gene            3346..4071
                     /locus_tag="VIG70_16090"
     CDS             3346..4071
                     /locus_tag="VIG70_16090"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEY8252119.1"
                     /translation="MASELHEIKELEGDLQPPALPVREALATACRVLAAEGHESGLAG
                     QVTARGEKSGTWWTLPFGYGFDEATAERMVLVDEDLKPLSGGRPNPGVRFHIWIYRKR
                     RDVQAIVHTHAPHASALAATGKPLKTIHMDSAMLHGTAHLAEWPGVPVADDEGRIISG
                     ALGDAKTILLANHGLLTTGTYVEEAAYLAVFFERAARMQLRAMAAGGFKEVKPELAAE
                     ARDFLLQASIVKGTFAYWARRCG"
     gene            4068..4961
                     /locus_tag="VIG70_16095"
     CDS             4068..4961
                     /locus_tag="VIG70_16095"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8252120.1"
                     /translation="MTQRARAFAFLALANLLWAGNWVIGRALRDAFDPVSLNFWRWVI
                     AVVVLAPFALPGLAAKRAVIRRHAAILALLAVLSVSIFQSLVYLGLQSTTAVNAVLIN
                     CAGPLFILLFAWLIDGERASLRQLAGFLVSVAGILVILSRGEPARLALLEFHSGDAWI
                     VLAIAIWGAYSVLLKRRPPELGGLHFLFVISAAGVLFLAPVFAFQALQAPPRLPTLPE
                     ALAVLYVGLAASVAAYLFWNRGTAIVGANAAGFTLYLLPTFGTVLAIAFLGETFGVFH
                     AVGTATIVAGIVLATFRRGAR"
     gene            complement(4936..5865)
                     /locus_tag="VIG70_16100"
     CDS             complement(4936..5865)
                     /locus_tag="VIG70_16100"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8252121.1"
                     /translation="MRRARRPGAPRRGHSQRSGKKMKLGFIGIGLMGRPMALRLLAAG
                     HEVTVWNRSRDKLAPVVEKGARAADSPAGVARACEILMTCVTDQHAMEEVLFGREGVA
                     HGAQRGSVVVDFSSIAPGAARELASRLEQQGIGLVDAPVSGGVAGAEKGTLAIMAGGR
                     AEHVERVRPVVMHLAQRFTRMGDSGAGQTTKLCNQIIVGSLIAVIAEAVRLAEAAGVD
                     ARQLPEALKGGFADSLPLQVFGARMAARTFEPSIGAVSIMLKDLENAAAVAKEKTVPL
                     PMARTAAELYRLLAAQGKAEKELSTIIELLSGK"
     gene            complement(5799..7475)
                     /gene="ilvD"
                     /locus_tag="VIG70_16105"
     CDS             complement(5799..7475)
                     /gene="ilvD"
                     /locus_tag="VIG70_16105"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY8252122.1"
                     /translation="MSTKKLPSRITVEGLDRAPHRAFLRALGLKDADLEKPFVGVAST
                     DSRVTPCNALLGELAREACAAVRAAGGVPFDFASASVADSMSMNHAGMRYSLVSREII
                     ADGVEAVARGHAYDALVTFAGCDKTLPGMMMAMVRLNLPAVFVYGGAALPGTWKGRDV
                     TVLDTYEAVGAVLAGDLSKKELDELERVCLPTLGSCPGQFTANTMAMVSETLGLAPPG
                     SATLPAVDSLRKDLARKAGETVMQLLKSGGPLPRDLVTKKSLENACAAVAATGGSTNA
                     ALHIPAIAHEAGIRFSMDDFARVAKRTPLIADMKPGGRFLAKDLHAVGGVYSVLKALL
                     THGALHGDCLTLSGNSLERELESHAGADGSVVRKEPIMKTGGLVVLKGNLASDGALIK
                     VAGLKSLVFEGPARVFDSEEACTEVVKQGKYNPGEVLVIRYEGPRGGPGMREMLGVTA
                     LLYGQGMGEKVALLTDGRFSGATRGMMVGYASPEAAAGGPLALVRNGDRILIDASAGI
                     LDLKVPKAELARRKAKAPKRLPLNGVLEKYAALVGPAHLGAVTHSGAGKK"
     gene            complement(7438..>8058)
                     /locus_tag="VIG70_16110"
     CDS             complement(7438..>8058)
                     /locus_tag="VIG70_16110"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8252123.1"
                     /translation="AGHCPKFVFASSIAVYGGVDEVTDETPVAPRLSYGAQKLVCEVL
                     IDDYTRRGYVDGRALRLPAVIVRPGTANTAVSGWSSAIIREPLAGRDYVCPVRPDTRL
                     ACISVKKVVESFIHAERLPAERLGGRRTLLLNGISISAQQMWDAVKSKATGKVRFAPD
                     PVIQPLMDRAPKKTFSKRSHGLGFPASASIEEIVREYEEAALAHHG"
BASE COUNT         1216 a         2809 c         2615 g         1418 t
ORIGIN      
        1 tcgagtgagc gggcaaactt catgcttggc aaagcattct gccaagcaat ctacaccaaa
       61 acaagggact gcggcgcgaa gctctagctg gtcttgtcga ccgcctgctt gaagccctga
      121 cccgcggcga acgccgggat ggtcttcgcc gagatcttga tctccttgcc gttcaccggg
      181 ctgcgcccgg cgcgcgcctt gcgggtgcgc ggcaggaaag tgccgaaccc gaccagggtg
      241 acgcgcttgc ccttcgccac gttgctcacg atcgcctcga acaccgcgtt caccacttcg
      301 ttggccgcga ccttgctgac gtttccggct ttagccgcga cctggaccag atcggacttg
      361 gtgaccacgt gtggcatgcg ctctcccctg actttcatcc ctacgtgcga gcccgcatcg
      421 tatccaaatc gaaatcgaaa cacaacgcgg gtggcgtgat tttgcgcacg ccgcggggac
      481 aaacatttat gatgcgggta tcggagagga gaacacatga tcaggtcatt gaccgtagcg
      541 tttactgcgg cgctcgcggc ggcgctctgc gtccccgccc acgcgcaaag cacgctcact
      601 tattcgtcgt gggtctcgcc gcagcaccat ctcagcgtct ggcaggcgaa ctggaccgcg
      661 gaagtcgaga aggcgaccgg cgggcgcgtg aagttccagt cgctgcccaa gcatccgtcg
      721 gcgccgccgg gaacgttcga cgcggtgcgc gacggcctga tggatctttc ctacgttacc
      781 gcgagctaca cgcccgcgcg ccacgtgatg ccgctgatgc ccgagctgcc cggcgccggc
      841 gacaccgccg aggtcaactc ggtcgcctac tcgcgcatcc actggaagca cttccagaag
      901 ctcggcgagt acaagggggt caagctgctc gcggtctgga cgcacggccc gggccagctg
      961 ttcaccaaga agacggtcgc cggcatcggc gacatccagg gtctcaagat ccgcaccggc
     1021 ggcggcgtcg ccgagaaggt ggccaacgcg ctgggcgcct cggccttcgt caagcccgcg
     1081 cccgagtcct acgagctgct gaagtccggc gtggccgacg gcgtgttctt cccgctcgag
     1141 tcgatcgtct cgttcaagct cgacacggtg ctcgagcagg ccacgatctt ccccggcggc
     1201 atgtacagct cggcgttcgg cttcttcatg aacgaggaca agtggaacaa gctggcgaag
     1261 caggaccagg cgatcatcga gaagttctcg tacgagtacg ccgcgcgcag caacggcaag
     1321 tcgtgggacg cggccgacca gaagggcctc gacgggctga agaaggccgg cgtcaagatc
     1381 atccaggccg acgccaagct gctcgccgag gtgcggaagc gctcggcgcc gatcatcgac
     1441 gactgggtga agcaggcgag cgccaagggc atcgacgcca aggcggtact cgccgagttc
     1501 cgcgctgagc tgaagaaggt cgccgccgag agcaagaagt agccgggggt ccgggcccct
     1561 gcgagttccc ggggccctcc cctcccactt cgtggggtcc tcccgtctct cccgaacctc
     1621 ctaacactct ctgggagcgg cgggtcgacg cggttcttgg cgtcgccgcc tcggcaatcc
     1681 tgttcgcgat gatgtgcctc acgttcgtcg acgtggtggc gcgctacgtc ttcaaccggc
     1741 cgctgcgcgg tgcgttcgag gtcaccgagc tgctgctcct ggtgctcatc ttcggcggcc
     1801 tgccgctggt ttcgcacgcg gacgagcacg tcaccatgga cttcatcgac cggctcctcg
     1861 gccgcgggct cgacctgtgg cagcgcgcag tgcacgcgct gtgcgcgggc gtcatgttcc
     1921 tgctcacctg gctggtgtgg atcaaggcga accgcatttc ggcgtacgcc gacgccaccg
     1981 acgtgctgcg catcctctac gggcctttcg tgtacttcat ggccgtgatg atcgcgctgg
     2041 cggggctgat tcacctctac aaggcatttg aatggccgaa ggactgatcg gcctcgcggc
     2101 gatgctggcg atcgcgttcc tgcgcgtgcc gatcgcgctc gcgatgggca tcgtcggcat
     2161 catcggctac gcctacatgc gcgactggaa cttcaccacc gcgttcgcgg tggcgcagac
     2221 caagatctac gagaccgggc gcaactacac gctctcggtg gtgccgcttt tcatcctgat
     2281 gggcaacttc gtcacgcgcg ccggcatgtc gcaggagctg ttccgcgccg cgtacgcatt
     2341 catcgggcac ctcaagggcg gcctcgcgat ggccacgatc ctcgcctgcg ccggcttcgg
     2401 cgcgatctgc ggctcgtcga tcgccaccgc cgccaccatg gcgaaggtcg cgtacccgtc
     2461 gatgaagcgc ttcggctatt ccgaccgcct cgccaccggc gcgatcgcct cgggcggaac
     2521 gctcggcatc atgatcccgc cctccaccat catggtgatc tacggcgtgt tcaccgagac
     2581 caacatcggg aaactcttcg ccgccggcgt gcttcccgga atcctcggcg ccaccctgct
     2641 gtgcctcacg gtgcgctacg tcacctggcg cgacccgaac gccggcccgc ccggcgagcg
     2701 ctcgagctgg cgcgagcgct gggcagccgt cagggacgtg tgggcagtcg cgttcctgtt
     2761 cctgttcgtg atgggcggca tctacggcgg cctgttcacc gcgaccgagg gcgcgggcat
     2821 gggcgccttc ggcgcgatgg cgttcgcgct ttggcgccgc gcgctcaact ggaagaccct
     2881 gtacgcggcg ctggtcgaga gcgcgcgcac ttcggcgatg ctgttcatga tcctgatcgg
     2941 cgcgctgatg ttcgccgact tcatcaacat caccagcatg ccggcagacc tgaagagctt
     3001 cgttacgcgc ttcgagctca gcccgatcct ggtcgtcgcg gcgatctgcg tgatctatgt
     3061 gctgctcggc accgcgatgg aggagctctc gatggtgctg ctgaccctgc cggtgttctt
     3121 ccccgtgatc atgcacctgg gctacgaccc ggtctggttc ggcatcctca tcgtcgtggt
     3181 ggtcgagatc gggctgatca gcccgccggt ggggatgaac ctcttcgtcc tcaagaccct
     3241 gctgccgggc gtgccgacca ccaccgtgtt ccgcggcgtg atgccgttca tgctcgccga
     3301 ctgcgtgcgc ttcgcgctcc tcatcgcctt cccggcgatc tcgctatggc ttccgagctt
     3361 catgaaataa aggagctgga gggggacctc cagccgccgg cgcttccggt gcgtgaagcg
     3421 ctggcgacgg cctgccgcgt cctggccgcc gaaggtcacg agtcggggct cgccggccaa
     3481 gtcacggcgc ggggcgagaa atccggaacc tggtggacgc tgccgttcgg ctacggcttc
     3541 gacgaagcaa ccgcagagcg catggtcctc gtcgacgagg atctgaagcc gctctcgggg
     3601 ggacggccga atcccggcgt gcgcttccac atctggatct atagaaagcg ccgggacgtg
     3661 caagccatag tgcatacaca cgctccgcac gcttccgccc tggctgctac gggcaagccg
     3721 ttgaagacca tccatatgga cagcgccatg ctgcacggca cggcgcatct ggccgaatgg
     3781 cccggcgtcc cggtggccga cgacgaaggt cgcatcatct cgggcgcgct cggcgacgcg
     3841 aaaaccatcc tgctcgccaa ccatggcctg ctcacgaccg gaacttacgt tgaagaagcg
     3901 gcgtacctcg cggtgttctt cgagcgcgcc gcgcgcatgc agctgcgcgc catggcggca
     3961 ggcggtttca aggaagtgaa acccgagctc gccgccgagg cgcgcgactt cctgcttcaa
     4021 gcgtcgatcg ttaaggggac tttcgcctac tgggcgcgcc gctgcgggtg acccaacgcg
     4081 cgcgggcctt cgcatttctc gcgctggcca atctgctctg ggccggcaac tgggtgatcg
     4141 gccgcgcgct gcgcgacgcg ttcgacccgg tctcgctcaa cttctggcgc tgggtcatcg
     4201 ccgtagtggt gctcgcgcct ttcgcgctgc ccggcctcgc cgcgaagcgc gcagtgatcc
     4261 gccggcatgc cgccatcctc gcgctgctcg cggtgctgag cgtctcgatc ttccagagcc
     4321 tggtctacct cggcctgcaa tcgaccaccg cggtgaacgc ggtgctgatc aactgcgcgg
     4381 ggccgctctt catcctgctg ttcgcctggc tgatcgacgg cgagcgcgcg tcgctgcggc
     4441 agctcgccgg gttcctggtc tcggtcgcgg ggatcctggt catcctgtcg cgcggcgagc
     4501 cggcccggct ggcgctgctc gagttccact cgggcgacgc ctggatcgtg ctggccatcg
     4561 cgatctgggg cgcgtattcc gtcctgctga agcggcgccc gcccgagctc ggcggcctgc
     4621 atttcctgtt cgtgatctcc gccgcgggcg tgctgttcct cgcgccggtt ttcgccttcc
     4681 aggcgctgca ggcgccgccg cgcctgccga ccctgcccga ggcgctcgcc gtgctctacg
     4741 tcggcctcgc cgcctcggtc gccgcctacc ttttctggaa ccgcggcacg gcgatcgtcg
     4801 gcgcgaacgc cgcgggcttc accctctacc tgctgcccac cttcggcacg gttctcgcca
     4861 ttgctttcct cggcgagacg ttcggcgtat tccacgccgt cggcaccgcg accatcgtcg
     4921 cgggaatcgt cttggctact ttccggagag gagctcgatg atggtcgaaa gctccttctc
     4981 tgctttgcct tgggcggcga gcagccggta gagctcggcc gcggtgcgcg ccatcggcag
     5041 cggcaccgtt ttctccttcg ccaccgcggc cgcgttttcc aggtccttca gcatgatgga
     5101 gacggcgccg atcgagggct cgaaagtgcg cgccgccatg cgcgcgccga agacctgcag
     5161 cggcagggaa tccgcgaagc cgcctttcag cgcctcgggc agctgcctgg cgtcgacgcc
     5221 cgccgcctcg gccaggcgca ccgcttcggc gatcaccgcg atgagcgagc cgacgatgat
     5281 ctggttgcaa agcttggtcg tctgcccggc gcccgagtcg cccatgcgcg tgaaacgctg
     5341 cgcgaggtgc atcaccaccg gccgcacgcg ctcgacgtgc tcggccctgc cgcccgccat
     5401 gatcgcgagc gtgcccttct cggcgcctgc gacgccgccc gacacggggg catcgaccag
     5461 accgatgccc tgctgctcca gcctcgaggc aagctcgcgc gctgcgcccg gcgcgatgct
     5521 cgaaaagtcg acgaccacgc ttcccctctg cgcaccgtgg gccacgccct cgcgaccgaa
     5581 cagcacctcc tccatcgcgt gctggtcggt cacgcacgtc atcaggatct cgcaggcacg
     5641 cgccacgcct gccggagaat ccgcggcgcg cgcgcctttc tcgacgaccg gcgcgagctt
     5701 gtcgcggctg cggttccaca ccgtcacctc gtggccggcc gcgagcagcc tgagcgccat
     5761 cggccgcccc atcaacccga taccgataaa tcccagcttc atttttttcc cgctccgctg
     5821 tgagtgaccg cgccgaggtg cgccgggccg acgagcgcgg cgtatttttc cagcacgccg
     5881 ttcagcggca agcgcttcgg cgctttcgct tttcgccttg caagttctgc cttggggacc
     5941 ttcagatcga gtattcccgc agaggcatcg atcaggatgc ggtcgccatt ccgcaccagc
     6001 gcaagcggcc cgccggcggc ggcctcgggc gaggcatagc cgaccatcat gccgcgcgtc
     6061 gcgccggaga agcgcccgtc ggtgagcagc gcgaccttct cgcccatgcc ctggccgtag
     6121 aggagcgccg tgacgccgag catctcgcgc atccccggcc cgcctctggg tccttcatag
     6181 cggatgacca ggacttctcc cgggttgtat tttccttgct tgaccacctc ggtgcaggct
     6241 tcctcggagt cgaacacccg cgccggcccc tcgaacacca gcgacttcag gcccgccacc
     6301 ttgatgagcg caccgtcgga ggcgaggttg cctttgagga cgaccaggcc gccggtcttc
     6361 atgatcggct ccttgcgcac cacgctgccg tctgcacccg cgtggctctc cagttcccgc
     6421 tcaagggagt ttccggaaag cgtgaggcaa tcaccgtgga gggcaccgtg cgtcagcaga
     6481 gccttgagca ctgaatagac gccgcccacg gcgtgcagat ccttggcgag gaagcgcccg
     6541 ccgggcttca tgtcggcgat gagcggcgtg cgcttcgcga cgcgcgcgaa gtcgtccatc
     6601 gagaagcgga tgccggcctc gtgcgcgatc gccgggatgt gcagcgcggc gttggtcgag
     6661 ccgccggtgg cggcgactgc ggcgcacgca ttctccaggc ttttcttcgt caccaggtcg
     6721 cgcggcagcg gaccaccgct cttgagaagc tgcattaccg tctctcccgc ttttcgggca
     6781 aggtctttac gaagagaatc gaccgccggc aaggtcgcgg agccgggagg cgcgaggccg
     6841 agcgtctccg acaccatcgc catcgtgttg gcggtgaact ggccgggaca ggagccgagc
     6901 gtgggcaggc agacccgctc cagctcgtcg agctctttct tggagagatc gcccgcgagc
     6961 acggcgccca cggcttcata ggtgtcgagc acggtcacgt cgcgcccttt ccacgtcccg
     7021 ggcagcgcgg cgccgccgta gacgaagacc gccggcaggt tgagccgcac catcgccatc
     7081 atcatcccgg gcagcgtctt gtcgcagccg gcgaaagtga cgagcgcgtc gtaggcgtga
     7141 ccgcgcgcca ccgcctcgac gccgtcggcg atgatctcgc gcgacaccag cgagtagcgc
     7201 atgccggcgt ggttcatcga catcgagtcg gcgaccgacg cggaggcgaa atcgaacggc
     7261 acgccgccgg ccgcgcgcac cgccgcgcac gcttcgcgcg cgagctcgcc gagcagcgca
     7321 ttgcacggcg tgacgcggct gtcggtcgac gccacgccga cgaacggttt ttcgagatcc
     7381 gcgtccttca accccagggc gcgcaggaac gcccggtgcg gcgcgcgatc gagcccctca
     7441 accgtgatgc gcgagggcag cttcttcgta ctcacggacg atttcttcga tgctggcgct
     7501 ggcgggaaaa cccaggccat ggcttctctt cgagaaggtc ttcttcggcg cgcggtccat
     7561 gagcggctgg atcacgggat cgggcgcgaa gcgcactttg ccggtcgcct tgcttttcac
     7621 ggcatcccac atctgttgcg cgcttatgga gatgccgttg aggagcagcg tgcgccggcc
     7681 gccgaggcgc tcggcgggaa gcctctcggc gtggatgaac gactcgacga ccttcttgac
     7741 ggagatgcac gcgagccgcg tatcgggcct gacgggacag acatagtcgc ggcccgcgag
     7801 cggctcgcgg atgatcgcgc tcgaccagcc cgagaccgcc gtattggcgg tgccgggacg
     7861 gacaatgacc gcgggcagac gcagcgcgcg cccgtcgaca tagccgcggc gcgtgtaatc
     7921 gtcgatcagg acctcgcaca ccagcttctg cgcgccgtag gaaaggcgcg gcgccacggg
     7981 ggtttcgtca gtcacttcat cgacgccccc gtaaacggcg atcgacgagg cgaacacgaa
     8041 tttcgggcag tgcccggc
//
LOCUS       DATBSZ010000253         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_389282, whole genome shotgun sequence.
ACCESSION   DATBSZ010000253 DATBSZ010000000
VERSION     DATBSZ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389282"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VIG70_16115"
     CDS             complement(<1..233)
                     /locus_tag="VIG70_16115"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HEY8252124.1"
                     /translation="MSKELEKILQKLEQLLPPPPPEPNWKTSVAFRWRSGGRGRPGWL
                     QPVRHVHRIKLSDLRGIDKQIERVEQNTRQFIDG"
     gene            complement(243..1439)
                     /gene="argJ"
                     /locus_tag="VIG70_16120"
     CDS             complement(243..1439)
                     /gene="argJ"
                     /locus_tag="VIG70_16120"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEY8252125.1"
                     /translation="MAVNLAVPQADSLHPVPGVELGVAMAGVKKPGRKDLLVMRLAPG
                     TAVAGVFTRNRFCAAPVILAKKHLKNEITALVVNTGNANAGTGDDGVKRALQVCDALA
                     GHLGCEVHEVLPFSTGVIMEPLPAERIIAALPKALLAKSDWLSAAEAIMTTDTVPKAF
                     SKKVRLSRGEVRVTGIAKGAGMIRPDMATMLGFIATDARISRANLQDLLERVADRSFN
                     CISVDGDTSTNDSLILAATGRGPAPASGRDRLKLEAAVTDVARSLAQAIVRDGEGATK
                     FVTVRVERGRSVEECRKIAYAIAHSPLVKTAFFASDPNLGRILAAVGNTGVEVNNMSL
                     YLDEVLVASAGGRHPAYKEEQGVAAMKKPEFTVRAVLNRGKADATVWTCDLSFDYVKI
                     NAEYRT"
     gene            complement(1465..>2189)
                     /locus_tag="VIG70_16125"
     CDS             complement(1465..>2189)
                     /locus_tag="VIG70_16125"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY8252126.1"
                     /translation="SNDVSGRISDLRAGVVTDTLRAHVPADSVEEQWDIAGLETALAA
                     ELELSLPLREWLEKEPELTDDSMLERIIERADAAYRDKIPAGAEEQFRQYERYLMLQI
                     LDSHWREHLAALDHLRQGIHLRGYAQKNPKQEYKREAFELFGAMVEAVKLEVTKTLTA
                     VQIRTQEDIKPDDASHVENVRPIHPTSDGGAQAEPDYNGDVAVAEAPKQRPIRVGQKV
                     GRNDPCPCGSGKKYKHCHGKLS"
BASE COUNT          310 a          731 c          733 g          415 t
ORIGIN      
        1 ccgtcgatga actggcgcgt gttctgctcg acgcgctcga tctgcttgtc gatgccgcgc
       61 aggtcggaaa gcttgatgcg gtggacgtgg cgcaccggct gcagccatcc cgggcggcct
      121 cggccgcccg agcgccagcg gaaggcgacg ctcgtcttcc aatttggctc gggcggcgga
      181 ggcggcagga gctgctcgag cttctgcagg attttttcga gttctttgct caagtcccgc
      241 ttctatgtcc gatattcggc gttgattttc acgtagtcga acgataggtc gcaagtccag
      301 acggtggcgt cggccttgcc gcggttcagc accgcgcgca cggtgaactc gggtttcttc
      361 atcgcggcca cgccctgctc ctccttgtac gcggggtggc gcccgccggc gctcgccacc
      421 aggacctcgt ccaggtagag gctcatgttg ttcacttcca cgccggtatt gccgaccgcg
      481 gcgaggatcc gcccgaggtt cgggtcggag gcgaagaaag cggtcttcac cagcggcgag
      541 tgcgcgatcg cgtacgcgat cttccggcat tcttcgacgc tgcgtccgcg ctcgacgcgc
      601 acagtgacga acttggtcgc gccttcgccg tcgcgcacga tcgcctgcgc gaggctgcgc
      661 gcgacgtcgg tgaccgcggc ttccagcttg agccggtcgc ggcccgaagc cggcgcgggg
      721 cctctgccgg tcgcggcgag gatcagggaa tcgttggtcg aagtgtcgcc gtcgacgctg
      781 atgcagttga acgaccggtc cgcgacgcgc tcgagcaggt cctggagatt cgctctggaa
      841 atgcgcgcgt cggtggcgat gaagcccagc atcgtcgcca tgtccggccg gatcatgccc
      901 gcgcccttcg cgatcccggt gacgcgcacc tcgcctcgcg aaagccgcac cttcttggag
      961 aaggccttcg gcacggtgtc agtcgtcatg atcgcctcgg ccgcggacag ccagtcgctc
     1021 ttggcaagca gcgctttcgg cagcgcggcg atgatgcgct cggccggcag gggctccatg
     1081 atcaccccgg tcgagaacgg cagcacctcg tgcacttcgc agccgagatg gccggcgagg
     1141 gcatcgcaga cctgcagcgc gcgcttcact ccgtcatcgc cggtgcccgc gttggcgttg
     1201 ccggtattga ccaccagggc ggtgatctca ttcttcaggt gctttttcgc aaggatgacc
     1261 ggagcggcgc aaaagcggtt ccgcgtgaac acgccggcca ctgcagtgcc cggcgcgaga
     1321 cgcatgacca gcaggtcctt gcgccccggt ttcttgacgc ccgccatcgc gaccccgagc
     1381 tctacgcccg ggacgggatg cagggaatcg gcctgcggga ctgcgagatt aacggccacg
     1441 gtcaaccgag atgattcgac accatcaact cagcttgccg tggcaatgct tgtacttctt
     1501 gcccgagccg caggggcagg gatcgttgcg accgaccttc tgccccacgc ggatcggcct
     1561 ctgcttcggc gcctcggcga ccgcgacgtc gccgttgtag tcgggttccg cttgagcgcc
     1621 gccgtcggag gtcgggtgga tcggccggac gttctcgacg tgggacgcat cgtcgggctt
     1681 gatgtcttcc tgggtgcgga tctgcaccgc agtgagggtc ttggtgacct cgagtttcac
     1741 cgcctcgacc atcgcgccga acagctcgaa cgcctcgcgc ttgtactcct gcttcgggtt
     1801 cttctgcgcg tagccgcgca ggtggatccc ctggcgcagg tgatcgagcg ccgccaggtg
     1861 ctcgcgccag tgcgaatcga gaatctgcag catcaggtag cgctcgtact ggcggaactg
     1921 ctcttccgcc ccggccggga tcttgtcgcg gtacgcggca tcggcacgct cgatgatgcg
     1981 ctcgagcatc gagtcgtcgg tgagctcggg ttccttctcg agccactcgc gcaggggcag
     2041 tgacagctcc agttccgccg ccagcgcggt ctccaggccg gcgatgtccc actgctcctc
     2101 cacgctgtcc gcgggcacgt gcgcgcgcag cgtgtcggtc acgacgcccg cgcgcaggtc
     2161 gctgatgcgg ccggagacgt cgttcgatt
//
LOCUS       DATBSZ010000254        22250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1209264, whole genome shotgun sequence.
ACCESSION   DATBSZ010000254 DATBSZ010000000
VERSION     DATBSZ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 22250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22250
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209264"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..843)
                     /locus_tag="VIG70_16130"
     CDS             complement(64..843)
                     /locus_tag="VIG70_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEY8252127.1"
                     /translation="MAIEISARKREMHGTNAARRLRRKGLVPGIIYGGEKDALNIELD
                     HQALSLNLRNERFHASILTLEVAGAKEQVLLRSVSMHPYKLQVQHVDFQRVLKNKKLH
                     MKVPLHFVNAEKSPGVKDQGGVASHVLNELDITCFPDDLPEFIEVDLINLAVGKSLHV
                     RELALPKGVEFALKKDEDPVVATVVVPTLITEEEEAAAAAAAVAPSEIPTTEQAAEAK
                     EGEAAAEGAKPGEKPAAKGAEKPAAKGAEKPADKADKKDKK"
     gene            complement(870..1832)
                     /locus_tag="VIG70_16135"
     CDS             complement(870..1832)
                     /locus_tag="VIG70_16135"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY8252128.1"
                     /translation="MTNHAALERLMVFTGNANPRLAHDVAKGLNLSLGRAIVGKFSDG
                     EVMVELLENVRGKDVFVLQSTCAPTNDNLMELLVMIDALRRSSAARVTAAIPYFGYAR
                     QDRRPRSARVAISAKVVANMLASVGVARLLTMDLHADQIQGFFDIPVDNIYAAPVLLG
                     DVWKQRYDDLIVVSPDVGGVVRARALAKRLESDLAIIDKRRPKANVSEVMNVIGEVKG
                     RTCVIMDDMVDTAGTLVKAAQVLKEEGATRVVAYCTHPVLSGGAVERIAKSDLDELVV
                     TDTIPLGDDARACKKIRILTVSGLIAETILRIYTEESVSSLFYE"
     gene            complement(1864..1940)
                     /locus_tag="VIG70_16140"
     tRNA            complement(1864..1940)
                     /locus_tag="VIG70_16140"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1904..1906),aa:Gln,seq:ttg)
     gene            complement(1954..2793)
                     /gene="ispE"
                     /locus_tag="VIG70_16145"
     CDS             complement(1954..2793)
                     /gene="ispE"
                     /locus_tag="VIG70_16145"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY8252129.1"
                     /translation="MELTWYPAPGKLNLFLHVLGRRPDGYHELQTVFRFIDRNDRVGL
                     SVRGDGEVRFSGMFGEDNLCVRAARLLKSQTGARHGCDIALEKSLPIGGGLGGGSSNA
                     ATVLVVLNKLWRLGLTRQRLMQLCLPLGADIPFFIYGRSAIGEGIGEQLRVLELPGAW
                     YLVLTPQVSVSTKETFESALTHRSKRLKIPPFLSGQGANDLEPAVVARYPDVAAGLAW
                     LRQHRPQARMTGSGACVFAEFETEAEARALYEAALQADLPGEMRGFVAKGLERHPLYD
                     LAD"
     gene            complement(2784..3266)
                     /locus_tag="VIG70_16150"
     CDS             complement(2784..3266)
                     /locus_tag="VIG70_16150"
                     /inference="COORDINATES: protein motif:HMM:NF015512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEY8252130.1"
                     /translation="MKRILVLAALLAACAHVEIKPPEGPLEFSLTGRIAARSGQESFT
                     GNISWRHARDGDELLISTPTGQGVAQILRQGDAVVLKTSEPREYRSADSEELTARVLG
                     FRLPIEGLAETVQGRPSPVLEDRGWKVEYQEYDAQRRPTRLRLTNVGAEVRLAISQWN
                     "
     gene            complement(3273..4952)
                     /locus_tag="VIG70_16155"
     CDS             complement(3273..4952)
                     /locus_tag="VIG70_16155"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8252131.1"
                     /translation="MVKRLIGAAALSFAFGLAAAQVPGPSPDKSVEELSEPTLYDFLL
                     GEIALQRGDLPLAAKTYLDLARRTRDPRVARRAVEVASQAKQPELALEAARLWHDIEP
                     TSAHALQVLAATLVAAKRVDEAGPVLEKLFAAEGVSRENAFMQVNRLLGSNPDKAANL
                     RAIRNLAAKYPQMPQAHFAVAQAAAVAGEDDAAIAAAREAQKLQPEWELAVLLEAQVL
                     QKRSNAAAAKRLGEFVEKYPQSRDVRLSYARVLVLDKRLPEARKQFEAVAAANPKNPE
                     VIYAVGLLAYQLKDYSAAEGYMKRVLDLGYRDPEGVRYLLGQMAEEQKAWPRAIEWYE
                     GIQNGDHVMPARMRTANAIAKQGKLDEARAYLKRVAEENPEDQIQLTVAEAQLLRDAQ
                     RHGDAFSLLNEALGKQPDQPDLLYDLALTAEKLERFDLLESNLRKLIEVKPDHAHAYN
                     ALGYSFAERNTRLPEARKLVEKALELAPEDYFIIDSLGWVLYREGDLKGAARELRRAY
                     GGRPDAEIGAHLGEVLWMLGERDEARRVWDESLKAGPENETLQKTIKRLKK"
     gene            5007..5816
                     /gene="mutM"
                     /locus_tag="VIG70_16160"
     CDS             5007..5816
                     /gene="mutM"
                     /locus_tag="VIG70_16160"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY8252132.1"
                     /translation="MPELPEVEVTRRGLAPQLTGRTISGVAVREPRLRWPVSRDVLAL
                     AGRTVKKVRRRGKYLLVDCGDGHLILHLGMSGSLRVLPLETPAGKHDHFDLVLGDRLL
                     RLRDPRRFGAVLWAPGDAAAHPLLAHLGVEPLSRSLNPGRLHSLTRAHRTAIKQFLMD
                     GRRIVGVGNIYASESLFRARINPRTPARRLSIEKCDKLAAAIKHTLRAAIRAGGSTLR
                     DFVGVDGAAGYFQQRYWVYDRAGKKCRRCGAPIRKLQQGQRSTYYCPSCQK"
     gene            complement(5800..6285)
                     /gene="coaD"
                     /locus_tag="VIG70_16165"
     CDS             complement(5800..6285)
                     /gene="coaD"
                     /locus_tag="VIG70_16165"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY8252133.1"
                     /translation="MIRAVYPGTFDPLTRGHEDLVRRAGTLFDTIVVAIADSKAKRPY
                     FTLEERIAMAREVLSDMKNVEVVGFSGLLTDFVRKQRSRLVLRGLRAVSDFEYEFQLA
                     GMNRNLYPEMETIFLTPSEQHMFISATLVREIAILGGDVTEFVHPSVAKRLKQKSTSG
                     S"
     gene            complement(6254..6847)
                     /gene="rsmD"
                     /locus_tag="VIG70_16170"
     CDS             complement(6254..6847)
                     /gene="rsmD"
                     /locus_tag="VIG70_16170"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY8252134.1"
                     /translation="MHSTNRNKPAKAGRIRIIAGEFRGRRIAVPDRPGLRPTPDRVRE
                     TLFNWLGQSLDGLACLDLFAGSGALGFEAASRGAARVVMVEHDRTVFEALKQTRDTIG
                     TGKVELVFDDAFAYLKRSQQGFDVVFLDPPFRQNALPAVFRQLRLQRGARVYVESDEP
                     YEAGEAWHELRRARAGQVSYQLLEWRGDDQGGLSGNV"
     gene            6728..7756
                     /gene="ftsY"
                     /locus_tag="VIG70_16175"
     CDS             6728..7756
                     /gene="ftsY"
                     /locus_tag="VIG70_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY8252135.1"
                     /translation="MGRRPQAGPVGDRDAPAAEFPGDDSNSSCLCGFIPIGRMHRSMV
                     ATSWAARLKAGLARTRDVLNTPVSELFVRRKIDEALYEELEAALLQADCGVAAAHALV
                     DELRRKKVDDGEALKGALKDAITDLLSPLEKRLEVEKARPFVIMIAGVNGSGKTTSIG
                     KLAKWLQSQGKSVMLAAGDTFRAAAREQLIAWGARNAVPVIAQESGDPGAVVFDALAA
                     ARARNVDVVIADTAGRLPTQLHLMEEIKKVKRVAAKGLEGAPHEVLLVLDANTGQNSL
                     AQVKAFDSALGLTGLILTKLDGTAKGGVVCAIAKEKPVPLLFIGVGEGIDDLRPFAAR
                     DFAEALVG"
     gene            7749..8399
                     /gene="ftsE"
                     /locus_tag="VIG70_16180"
     CDS             7749..8399
                     /gene="ftsE"
                     /locus_tag="VIG70_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY8252136.1"
                     /translation="MVSFSAVAKRYPGGLQALKDVSFSLEAGELAFLTGRSGAGKSTL
                     LKLIPAIERPSAGSVVVNGQNVSALKRSAIPYLRRNIGLVFQDQKLLYDRSAYDNVIL
                     PLAFSSHPPKEAARRARAALDKVGLLGREKSNPVQLSGGEQQRLAIARAVVNRPALLV
                     ADEPTANLDAESAARILEIFVAFNQVGVTVLIATHDQTLVARYGKKVLHLDAGRLA"
     gene            8396..9262
                     /locus_tag="VIG70_16185"
     CDS             8396..9262
                     /locus_tag="VIG70_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY8252137.1"
                     /translation="MSAWLRQHRQAFAAACRRLGWLNALVIGIALALPAGGYALLESL
                     RPAGARLALEPRMTLFLDAQVRRADAEALGKRLRADPRIAGVRFVPREEALKEMSAVQ
                     GLSEVIAALGRNPLPDALVVTSRENIADELARLPGVAHVQADAVWARRLAAAAAIVEL
                     GLWLLAALLGAGLVAVTFNTIRLQILTQRDEIEVSKLIGATDAFIRRPFYYLGLLQGL
                     VGGAVALALVVAALALLNREVSVLAESYGSSFRLGFPAEGALAVVAFAALLGWLGAQL
                     SVSRHLRAIEPM"
     gene            9422..10276
                     /gene="rpoH"
                     /locus_tag="VIG70_16190"
     CDS             9422..10276
                     /gene="rpoH"
                     /locus_tag="VIG70_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEY8252138.1"
                     /translation="MPTGSLEAYIQTVNRIPMLTGPEERELALRFRDHNDLEAARQLV
                     LSHLRLVVAVARGYLGYGLPHADLIQEGSIGLMKAVKRFDPDRNVRLVSFAIHWVKAE
                     IHEYILRNWRLVKLATTKAQRKLFFNLRSMKSGLAPMTVKDVERIAGELRVKPEEVVE
                     METRLSGQEIAFEADQDDEDGYAPANYLAADRDAEPLSQLEAKESEEGRAAGLEDALQ
                     SLDPRSRRIIETRWLREKDALTLHDLAAEFKVSAERIRQIEAKALEKMKKVMQSEPGA
                     PLALPSPT"
     gene            complement(10273..10857)
                     /locus_tag="VIG70_16195"
     CDS             complement(10273..10857)
                     /locus_tag="VIG70_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY8252139.1"
                     /translation="MRSFSRLFWSITICSAIAGCDSRPQFKSTDITGAPYGQSLELTD
                     HTGRARRLEDFRGKAVVLFFGFTHCPDICPTTLAEISQAIRSLGPDAERVQLLMVSVD
                     PERDTQDSLAKYVTAFDARFLGLRGDLAATRKVAAEFKIYFEKRKQGDSYSVDHSAQS
                     YVIDPQGRLRLLVRHDRIGQDLAPDLRTLLKNPG"
     gene            complement(10814..11674)
                     /gene="cyoE"
                     /locus_tag="VIG70_16200"
     CDS             complement(10814..11674)
                     /gene="cyoE"
                     /locus_tag="VIG70_16200"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY8252140.1"
                     /translation="MRARFRAFAALTKPRVVSLIVFTAVIGMFLATPGVVPMQALLAG
                     TIGIALVAGAAAAVNCLVEQKIDAVMSRTRWRPLPQGELSAAHTLVFAGALGGAGLWL
                     LYNFVNALTMWLTLATFVGYAIVYTVILKPATPQNIVIGGASGAMPPVLGWAAVTGEV
                     PIEAMLLFLIIFAWTPPHFWALALYRTEDYARAGVPMLPVTHGRKYTQLQVLLYTLIL
                     FAVSLLPYVAGMSGLAYLIAAAALGAVFLAYALRIYFAYSDALARKTFRYSIAYLAFL
                     FAALLVDHYL"
     gene            complement(11751..12206)
                     /locus_tag="VIG70_16205"
     CDS             complement(11751..12206)
                     /locus_tag="VIG70_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HEY8252141.1"
                     /translation="MLGTAAYLLGWSPGGATNYGELLDPRPVSGFDPLRGKWVLVSFD
                     AAECDAYCEKKLYFMRQVRRAQGKEMDRVERIWVVTDGGKPPAELLPGIEGTRLAAWS
                     SESFPGKATDHIYLVDPFGNLMLRFPRDPDPSKMIKDLQRLLKYSPRTK"
     gene            complement(12244..12747)
                     /locus_tag="VIG70_16210"
     CDS             complement(12244..12747)
                     /locus_tag="VIG70_16210"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEY8252142.1"
                     /translation="MAVEGEFLPRYTVYLDNKTRRGKAGYEIVTPMKIRGSSSHVLVN
                     RGWIEAGKTREVLPEVRTPAGELRLEGLALERLAQPLKLKEDKSRVRQSLDIAAYAAE
                     TGLRLEPRVIEQHSDLPDGLARDWPPHDAGVEKNEGYALQWYSLAALALILAVVLSFR
                     KIDVPSK"
     gene            12806..13072
                     /locus_tag="VIG70_16215"
     CDS             12806..13072
                     /locus_tag="VIG70_16215"
                     /inference="COORDINATES: protein motif:HMM:NF022587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEY8252143.1"
                     /translation="MPAAHAAAASASAQSRGRKEYPAGMRYVVIAMLVAIVASLGSAL
                     WYMLRPGKDPKRMVKALALRVGLSVTLFVLLMAGYYFGFITGRL"
     gene            complement(13067..13939)
                     /locus_tag="VIG70_16220"
     CDS             complement(13067..13939)
                     /locus_tag="VIG70_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY8252144.1"
                     /translation="MAHSASTPHGKQAHYSVPQPMPWPVMGSASLFMMALGAVFVFNG
                     MSGGWIGIAAGFMLLLYMMVRWFGEVISESEGGKYGRWEDVSFRWGMSFFIFSEVMFF
                     AAFFGALFWARVISVPDLASIESNALMWPGYTAHWPNAGPAFIEKFTPMGAWGLPAVN
                     TILLLSSGVTVTFAHWNLIANNRPKLLWWLGATVLLGVGFLVCQAIEYVHAFQELNLT
                     LHTGVYGSTFFMLTGFHGFHVTIGAIMLTVIWFRCLKGHFTPDHHFAFEGVAWYWHFV
                     DVVWLGLFIFVYWL"
     gene            complement(13955..14110)
                     /locus_tag="VIG70_16225"
     CDS             complement(13955..14110)
                     /locus_tag="VIG70_16225"
                     /inference="COORDINATES: protein motif:HMM:NF022622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HEY8252145.1"
                     /translation="MKTVLWGMVGIRRKADHEKTPLNPVHLAIAAIGFVVLFILTLVT
                     IVRIVTS"
     gene            complement(14119..14649)
                     /locus_tag="VIG70_16230"
     CDS             complement(14119..14649)
                     /locus_tag="VIG70_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY8252146.1"
                     /translation="MNAKRENRRLSRRLAIVAIAMFGFAFALVPFYNQICVALGINNL
                     DQPAEALNTQVDTSRTVTIELDSNVHGLPWRFRALVSEVKVHPGELAMVEYEIVNVRE
                     AAVTGQAVPSYGPQSAGEYFRKIECFCFTHQTLAAGETRRMPVVFMVDPKLPKDVNTI
                     ALSYTFFEVAGRGGKT"
     gene            complement(14646..14738)
                     /locus_tag="VIG70_16235"
     CDS             complement(14646..14738)
                     /locus_tag="VIG70_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HEY8252147.1"
                     /translation="MSKNAKTALILASIALAFFLGIIAKYWLLR"
     gene            complement(14752..16347)
                     /gene="ctaD"
                     /locus_tag="VIG70_16240"
     CDS             complement(14752..16347)
                     /gene="ctaD"
                     /locus_tag="VIG70_16240"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY8252148.1"
                     /translation="MSAVVHDHGHAHDDHGHHGPAAGLMRWVTTTNHKDIGTLYLWFS
                     FIMFSIGGLLAVGIRLELFSPGLQHMDPDFFNQLTTMHGLIMVFGAIMPVFVGFANWQ
                     IPMMIGAPDMAFARLNNWSFWLLPFAATLLVLSFFMPGGAPGVGWTMYAPLTVQMGIG
                     MDLTIFAVHILGMSSILGSINIITTILNMRAPGMTLMRMPLFVWTWLITAYLLVAAMP
                     VLAGAVTMTLTDRHFGTTFFNAAGGGDPVLYQHIFWFFGHPEVYIIALPGFGVISQVI
                     PAFSRKPLFGYASMVYATSAIAIISFIVWAHHMYTVGMPVTGQLFFMYATMIIAVPTG
                     VKVFNWVATMWKGSLTFETPMLFALGFLFMFTLGGFTGLVLAVVPIDIQMHDTYYVVA
                     HFHYVMVAGALFAAFAGVYFWLPKWTGRMYNERLGQIHFWLTLIFFNMIFFVQHFLGM
                     AGMPRRIPDYHPMFELWNKISSLGAFGMGLAQLLFLYIVVKTIRSGEPAPQRPWEGAD
                     SLEWTHLPTPPPYHTFETQPTIK"
     gene            complement(16353..17444)
                     /gene="coxB"
                     /locus_tag="VIG70_16245"
     CDS             complement(16353..17444)
                     /gene="coxB"
                     /locus_tag="VIG70_16245"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY8252149.1"
                     /translation="MIALAASSAAHAIAWYFQSPASAMAREIDWLHQVLMWIILVIFI
                     GVFGFMFYACFAHRKSKGHRAAQFHENTTVEILWTVIPALILIVIAWPVTKVVIAQKD
                     TSAPDLTIKVTGYQWKWGYDYLKGEGEGISFVSTLATPREQIEGKAPKGENYLLEVDN
                     EVVVPVGKKVRVLTTAADVVHAWWVPAFGAKQDAIPGFIRDLWFRADREGTFRSQCVE
                     LCGKEHGFMPIVVRVVSAPEYSKWVESQKKSMAAAADDPNKAWDLKELVARGEKVYAA
                     NCVACHQASGQGLAAMKAPALAGNKFVTGAEPGPIDTVLNGRPNTAMQAFKQQLSDTE
                     IAAVISYARNSWGNKASDVQPAEVKARRK"
     gene            complement(17477..17917)
                     /locus_tag="VIG70_16250"
     CDS             complement(17477..17917)
                     /locus_tag="VIG70_16250"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEY8252150.1"
                     /translation="MSAPSFSLVLKRNCSMSPKALAGVFAALALVVLLIGAGFALAGA
                     WLVLPFAGLEVLLLGGAYVAYARHAADYERLEIEPGGVSVEVAKAQGIARYRLVNAKV
                     SVEDGRVVLRDAKEELEVGRYLGAEARVELAAELQKRLIRGIAT"
     gene            complement(17953..18774)
                     /locus_tag="VIG70_16255"
     CDS             complement(17953..18774)
                     /locus_tag="VIG70_16255"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8252151.1"
                     /translation="MSARSPGIDRDLARRRFERAARTYGAASRLEGEVAARMLERLDY
                     MKLAPWRILDAASGPPRRELGRRYPGAQVIALDFAEAMLRATRKRIFGKNSLRVCGDL
                     ERLPLADGAVDFVWCNMALHWLPDPQRAFAEFARVLTPEGLLMFSTLGPDTLKELRAA
                     AGAARVHAFIDMHDLGDMLVANGFSAPVMDMEMLQIAYAAAGGLVEDLKNTGQINARA
                     DRPRGLAGKGFGERLRALPPRATYEVVYGHAWRTAKATRDANQDPKTVRIFKRMP"
     gene            complement(18749..20110)
                     /locus_tag="VIG70_16260"
     CDS             complement(18749..20110)
                     /locus_tag="VIG70_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8252152.1"
                     /translation="MWTVAQLAADLAAGRTTSRRLTEQALARIADPAGEGGRAFLKVY
                     ADSALADADTADRLRKSGVRRSPVDGLPVSLKDLFDVAGDVTRAGSKVHHQKAESDAA
                     AVARLRAAGAVFVGRTNMVEFAFGTTGINPHYGTPRSPWDRPKWNAAEPLTGRVPGGS
                     SSGAAVAQADGMCVMALGSDTRGSVRIPAAFCGVTGFKPTTRRVPREGAFPLSYTLDS
                     IGPLANSVACCAAYDAILAGEAPDALAELPARGLRLLVPKSAVMEGLDREVAEAFNSA
                     VERLKRAGAVVTEKPLPAFDRQSEYFKGGGFAGAEAYAIHRKNADRLSEYDPRVAKRV
                     LLGKDLSAADFVELTLLRTAYQREVEAALAPFDAFLLPTTPCVPPPIAEVNASDESYF
                     RWNMRILGIVGLVNFLDGCAVSLPCQAAGAAPVGLMVSGPAMSDRRILAVAAAIERTL
                     ARD"
     gene            20123..20785
                     /locus_tag="VIG70_16265"
     CDS             20123..20785
                     /locus_tag="VIG70_16265"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY8252153.1"
                     /translation="MRNFFAQLFGGSCFLCRGAATDLLCPSCDADLPRLAAPCCPRCA
                     LPSPRGEVCGRCLHEAPHYDATIAALAYAFPADVLVHALKFRGELALAGLLGGLLSRK
                     ISHQVDCIVPVPLSSARLRERGYNQSVEIARQLGAQLDLALCVRERDTPAQMDLPYAE
                     RKRNVRGAFRCTRSLGGTRVAVVDDVMTTGATLNEVASVLKQAGAAQVVNWVVARTLP
                     DV"
     gene            20778..21242
                     /locus_tag="VIG70_16270"
     CDS             20778..21242
                     /locus_tag="VIG70_16270"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY8252154.1"
                     /translation="MFNIVLVQPEIPPNAGNVIRLAANTGARLHLVEPLGFTLEDRQL
                     KRAGLDYHDLAHVTVHRDWETCRAALGERRLFALSTRGTRGYAEVEFRVGDTFVFGAE
                     TRGLSAQLLDEFPAAMRLRLPMLAGNRSLNLSNAVAVVVFEAWRQIGFLGSA"
     gene            complement(21243..22115)
                     /locus_tag="VIG70_16275"
     CDS             complement(21243..22115)
                     /locus_tag="VIG70_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252155.1"
                     /translation="MVNTSWNTQGVWTEPGVRFDLRFEYIDQDQPMAGDSKVGVGQIS
                     QHHDEVRTINRNWLATVDYAFNADWGISATLPLIDRDHTHIHNHHGAQLTESWDFREI
                     GDARMLGRRQWRSESPAAERVDFYGVNFGLKVPTGKRDVRNHEGALAERSLQPGSGTT
                     DLLVGGYFTRVLGSGSSWFADALVQSPFDARDNYEPGSRVSLDLGYRRELGSVLAGML
                     QLNLLYKDRDKGSEAEPANSGGTFLYVSPGISYMMSKRVQAYAFVQLPLYQYVNGVQL
                     TADWAGVLGVSTRF"
BASE COUNT         3676 a         7575 c         7326 g         3673 t
ORIGIN      
        1 ccgcgttgtg gcgcgtgcgc tcgtactcct tgcccgggtt gcccaggccg acaactagcc
       61 tgatcacttc ttgtcttttt tgtcagcctt gtcggcaggc ttctcggcgc ccttcgcggc
      121 gggcttctcg gcgcccttcg cggcaggctt ctcgccaggc ttggcaccct cggcagccgc
      181 ctcaccttcc ttcgcctcgg cggcctgctc ggtcgtcggg atctcggagg gcgcgactgc
      241 ggcggcagca gcggcggctt cttcttcctc ggtgatcagc gtcggcacca cgacggtcgc
      301 caccaccggg tcctcgtcct tcttcagggc gaactccacg cccttgggca gggcaagctc
      361 gcgtacgtgg agcgacttgc cgaccgcgag gttgatcagg tcgacctcga tgaactccgg
      421 caggtcgtcg gggaagcagg tgatgtcgag ctcgttcagc acgtggctcg cgacgccgcc
      481 ctggtccttc acccccggcg acttctcggc gttgacgaag tgcagcggca ccttcatgtg
      541 cagcttcttg ttcttcagca cgcgctggaa gtcgacgtgc tgcacctgca gcttgtacgg
      601 gtgcatgctc accgagcgca gcagcacctg ctccttcgcg ccggccacct ccagggtgag
      661 gatcgaggca tggaagcgct cgttcctcaa gttgagagac agggcctggt ggtcgagctc
      721 gatgttgagc gcgtccttct cgccgccgta gatgatgccg ggcacgaggc ccttgcggcg
      781 caggcggcgt gcggcgttgg tgccgtgcat ttcgcgcttg cgcgcgctga tttcgatagc
      841 catatggact tacctcagta gatgctcatt tactcgtaaa acagcgagct caccgattcc
      901 tcggtgtaga tgcgcaggat ggtctcggcg atcaggccgg aaaccgtcag gatgcggatt
      961 ttcttgcaag cgcgcgcgtc gtcgcccagc gggatggtat cggtgacgac cagctcgtcg
     1021 aggtccgatt tcgcgatgcg ctcgaccgcc ccgccggaaa gcacggggtg ggtgcagtag
     1081 gcgaccacgc gcgtcgcgcc ttcttccttc agcacctgcg ccgccttcac cagcgtgccg
     1141 gcggtgtcga ccatgtcgtc catgatgacg caggtgcgtc ccttgacctc gccgatgacg
     1201 ttcatcactt ccgagacgtt ggccttcggc cggcgcttgt cgatgatcgc caggtccgac
     1261 tccaggcgct tcgccagcgc ccgcgcgcgc accacgccgc cgacgtcggg cgagaccacg
     1321 atcaggtcgt cgtagcgctg cttccacacg tcgccgagca gcaccggcgc ggcgtagatg
     1381 ttgtcgaccg ggatgtcgaa gaagccctgg atctggtcgg cgtgcaggtc catcgtcagc
     1441 aggcgcgcga cgccgaccga ggcgagcatg ttcgccacca ccttggcgct gatcgcgacg
     1501 cgcgccgagc gcggccggcg gtcctgccgc gcgtagccga aatacgggat cgccgccgtg
     1561 acgcgcgccg ccgaggagcg cctgagcgcg tcgatcatca ccagcagctc catcaggttg
     1621 tcgttggtcg gcgcgcaggt cgactgcagc acgaacacgt ccttgccgcg cacgttctcg
     1681 agcagctcca ccatcacctc gccgtcggag aacttgccga cgatcgcgcg cccgagcgac
     1741 aggttcagcc ccttcgccac gtcgtgcgcg agccgcgggt tggcgttgcc cgtgaacacc
     1801 atcagccgct cgagagcggc gtggttagtc atcccggcgg tgttcatggc ggtcatggat
     1861 caatggctgg ggaggaagga ttcgaaccct cgtatggcgg aatcaaaatc cgctgcctta
     1921 accagcttgg cgactcccca gtgtaaagct cactcagtct gcaagatcgt aaagcgggtg
     1981 tcgctccaat cccttggcga cgaatccccg catctccccg ggtaagtcgg cctgtaatgc
     2041 ggcctcgtac agcgcgcgcg cttcggcttc ggtctcgaac tcggcgaaga cacacgctcc
     2101 ggaccccgtc atgcgcgcct gcgggcggtg ttgcctgagc cacgcaaggc ccgctgccac
     2161 gtcggggtat cgggcaacga ccgcaggctc gagatcgttc gctccctgcc ccgacaaaaa
     2221 gggcggtatt ttgagtcgtt tcgaccgatg tgtcaacgca gattcgaagg tttccttcgt
     2281 ggaaaccgag acttgcggcg tgaggacgag gtaccaggcg cccggaagct cgagcacgcg
     2341 caactgctcc ccgatgcctt ctccgatcgc gcttcttccg tagatgaaga agggaatgtc
     2401 cgcgcccagg ggaagacaaa gctgcatcag cctctggcgg gtcagcccga ggcgccacag
     2461 cttgttcagg accaccagaa ccgtggccgc gttggaagac ccgccgccca gcccgccgcc
     2521 gatcggaagg cttttttcca atgcgatgtc gcagccgtga cgcgctccgg tctgtgattt
     2581 caacaagcgc gccgcgcgca cgcacaggtt gtcttcgccg aacattccgg aaaaccggac
     2641 ctcgccgtcg ccgcggacgg aaagcccgac ccggtcgttg cggtcgatga agcgaaagac
     2701 cgtctgcagc tcgtggtagc cgtcaggccg gcgccccagg acgtgaagga agaggttgag
     2761 cttgcctggg gccggatacc aggtcagttc cactgcgata ttgccagccg cacctcggcc
     2821 ccgacattcg tgagccggag ccgggtgggc cggcgctgcg cgtcgtactc ctgatattcg
     2881 accttccagc cgcggtcttc gagcacgggc gagggtctcc cctgtacggt ctcggcgagc
     2941 ccctcgatcg gcaggcggaa accgagaact cgcgcggtga gttcctcgga gtcggccgag
     3001 cggtattcgc gcggctccga agttttcagc acgacggcat ccccctggcg caggatctgc
     3061 gccacgccct ggccggtcgg cgtgctgatg agcagctcgt cgccatcgcg cgcatggcgc
     3121 caagaaatgt tgccggtgaa ggattcctgg cccgagcgcg ccgcgatgcg gcccgtgagg
     3181 gaaaactcga gcgggccttc ggggggcttg atctcgacgt gagcgcaggc agcgagaagc
     3241 gccgccagca ccagaatacg tttcacgggt cgttacttct tcaaccgctt gatcgtcttc
     3301 tgcagggtct cgttttccgg gccggccttg agcgactcgt cccagacgcg gcgcgcctcg
     3361 tcgcgctcgc cgagcatcca cagcacctcg ccgaggtgcg cgccgatctc ggcgtcggga
     3421 cggccgccgt aggcgcggcg cagctcgcgc gcggcgccct tgaggtcgcc ttcccggtag
     3481 agcacccagc ccaggctgtc gatgatgaaa tagtcctcgg gcgcgagctc gagcgccttc
     3541 tcgaccagct tgcgcgcctc gggcaggcgg gtgttgcgct cggcgaacga atagccgagt
     3601 gcgttatagg cgtgcgcgtg gtccggcttc acctcgatca gcttgcgcag attcgactcg
     3661 agcaggtcga agcgctcgag cttctcggcg gtgagcgcga ggtcgtagag caggtccggc
     3721 tggtcgggct gcttgcccag ggcctcgttc agcagcgaaa aagcatcccc gtgccgctgc
     3781 gcgtcgcgca gaagctgtgc ctcggcgacc gtgagctgga tctggtcttc gggattctcc
     3841 tcggcgacgc gcttcaggta cgcgcgcgcc tcgtcgagct tgccctgctt cgcgatcgca
     3901 ttggcggtgc gcatgcgcgc gggcatgacg tggtcgccgt tctggatgcc ctcgtaccac
     3961 tcgatcgcgc gcggccacgc cttctgctcc tccgccatct gcccgaggag atagcgcacg
     4021 ccttccgggt cgcggtagcc gaggtcgagg acgcgcttca tgtagccctc ggccgcgctg
     4081 tagtccttca actgataagc gagcaggccg acggcataga tgacttcggg attcttggga
     4141 ttggcggcgg ccacggcctc gaactgcttg cgcgcctcgg gcaggcgctt gtccagcacc
     4201 agcacgcgcg cgtagctgag ccgcacgtcg cgcgattgcg ggtacttctc gacgaactcg
     4261 cccagccgct tcgccgccgc agcgttcgag cgcttctgca gcacctgcgc ttcgagcagc
     4321 acggcgagct cccactccgg ctgcagcttc tgcgcttcgc gcgccgccgc gatcgccgcg
     4381 tcgtcctctc ccgccaccgc cgcggcctgg gcgaccgcga agtgcgcctg cggcatctgc
     4441 gggtatttcg ccgcgaggtt gcggatggcg cgcaggttcg cggccttgtc cggattgctg
     4501 ccgagcaggc ggttcacctg catgaacgcg ttctcgcggc tcacgccctc cgcggcaaac
     4561 agcttctcga gcaccggccc ggcttcgtcg acgcgcttcg ccgccaccag cgtcgcggcc
     4621 aggacctgca gcgcgtgcgc cgaggtcggc tcgatgtcat gccagaggcg tgcggcctcg
     4681 agcgcgagct cgggctgctt cgcctggctc gccacctcga ccgcgcggcg cgcgacgcgc
     4741 gggtcgcgcg tgcgccgggc gagatccagg taggtctttg cggcgagtgg caggtcgccg
     4801 cgctgcaggg cgatctcgcc gagcaggaaa tcgtacagcg tcggctcgct cagctcctcg
     4861 acgctcttgt ccggggacgg gcccggaacc tgagctgcgg cgagaccgaa ggcgaatgac
     4921 agcgccgctg cgccaataag tcgtttgacc atgctggatg cgatccatct taggtatatt
     4981 tggtgccccg cacaagcgca cctggcatgc cggagctccc cgaagtcgaa gtcacgcggc
     5041 gcggtctcgc gccgcaactc accggccgca cgatcagcgg cgtggcggtg cgcgagccgc
     5101 ggctgcgctg gccggtgtcg cgcgacgtgc tcgcgctcgc gggccgcacc gtgaaaaagg
     5161 tccgccgccg cggcaaatat ctgctcgtcg actgcggcga cggtcacctc atcctgcacc
     5221 tgggaatgtc gggaagcctg cgcgtgctgc cgctggaaac cccggccggg aagcacgacc
     5281 acttcgacct ggtgctcggc gaccgcttgc tgcgcctgcg cgacccgcgc cgcttcggcg
     5341 cggtgctgtg ggccccgggc gatgccgccg cgcacccgct gctcgcgcat ctcggcgtcg
     5401 agccgctgtc gcgctcgctc aatccagggc ggctgcactc gctcacgcgc gcgcaccgca
     5461 cggcgatcaa gcagttcctg atggacggac ggcgcatcgt cggcgtcggc aacatctacg
     5521 cgagcgagag cctgttccgc gcgcgcatca atccgcgcac ccccgcaaga aggctgtcga
     5581 tcgaaaagtg cgacaagctc gccgcggcga tcaagcacac gctgcgcgcg gcgatccgcg
     5641 ccggcggctc gacgctgcgc gacttcgtcg gcgtcgacgg cgccgcggga tattttcagc
     5701 agcgctattg ggtgtacgac cgcgccggaa agaaatgccg ccgctgcggc gcgccgatcc
     5761 gcaagctaca gcagggccag cgctcgacct actactgccc tagctgccag aagtagactt
     5821 ctgcttcagc cgtttcgcca cgctcgggtg aacgaactcc gtcacgtcgc cgccgaggat
     5881 ggcgatctcg cgcaccagcg tcgccgagat gaacatgtgc tgctcggaag gcgtgagaaa
     5941 gatcgtctcc atctcggggt agaggttgcg gttcatgccc gcgagctgga actcgtactc
     6001 gaaatccgac accgctcgca gcccgcgcag cacgaggcgc gagcgctgct tgcgcacgaa
     6061 atccgtgagc aggccggaga acccgaccac ctcgacgttc ttcatgtccg acagcacctc
     6121 gcgcgccatg gcgatgcgct cctccagcgt gaagtacggg cgcttcgcct tgctgtccgc
     6181 gatcgccacg acgatggtgt cgaacagcgt cccggcgcgg cgcaccaggt cctcgtggcc
     6241 gcgcgtgagc ggatcaaacg ttcccggata gaccgccctg atcatcgcct ctccattcca
     6301 acagctggta gctcacctgg ccggcgcgcg cgcgcctgag ctcgtgccac gcttctcccg
     6361 cctcgtaagg ctcgtccgat tccacgtaga cgcgcgcccc gcgctgcagc ctgagttgtc
     6421 tgaacaccgc cggcagcgca ttctgcctga acggcggatc gaggaaaacc acgtcgaagc
     6481 cttgctggct tctcttcaag tacgcgaacg cgtcgtcaaa gacgagctcc acttttcccg
     6541 tgccgatcgt gtcgcgggtt tgcttcagcg cttcgaacac cgtgcgatcg tgctccacca
     6601 tcaccacccg cgccgcgccg cgcgacgcgg cctcgaagcc gagcgcgccg ctgccggcga
     6661 acagatcgag gcacgcgagc ccgtcgagcg actggccgag ccagttgaac agggtctcgc
     6721 gcacgcgatc gggcgtcggc cgcaggccgg gccggtcggg gaccgcgatg cgccggccgc
     6781 ggaattcccc ggcgatgatt cgaattcttc ctgcctttgc gggtttattc ctattggtag
     6841 aatgcaccga tcgatggtag cgacttcgtg ggccgcgcgc ctcaaggcag gactcgcgcg
     6901 cacgcgggat gtcctcaaca cgcccgtctc cgagctgttc gtccggcgca agatcgacga
     6961 agcgctctac gaagagctcg aagcggcgct gctgcaggcc gactgcggcg tagcggcggc
     7021 acacgcgctg gtcgatgaat tgaggaggaa aaaggtcgac gacggcgaag cgctgaaggg
     7081 cgcgctcaag gatgcaatca ccgatttgct ttctcccctc gagaagcgac ttgaggttga
     7141 aaaagcaagg ccgttcgtca tcatgattgc gggcgtgaac ggctcgggca agaccacctc
     7201 gatcggcaag ctcgcgaaat ggctgcagtc gcagggcaag agcgtgatgc tcgccgccgg
     7261 cgacactttc cgcgccgccg cgcgcgagca gctcatcgcc tggggcgcgc gcaacgccgt
     7321 gccggtcatc gcgcaggaat ccggcgatcc cggcgcagtg gtgttcgacg cgctcgctgc
     7381 cgcgcgcgcg cgcaacgtcg acgtggtgat cgccgatacc gccggcaggc tgccgacgca
     7441 gctccacctc atggaggaga tcaagaaggt gaagcgcgtg gcggcgaagg ggctcgaagg
     7501 cgcgccgcac gaggtgctgc tggtgctcga cgcgaatacc gggcagaact cgctcgcgca
     7561 ggtgaaggcg ttcgacagcg cgctcgggct gaccgggctc atcctcacga agctcgacgg
     7621 caccgccaag ggcggcgtgg tgtgcgcgat cgccaaggaa aagccggtgc cgctcctctt
     7681 catcggcgtg ggcgaaggca tcgacgacct gcggccgttc gcggcgcgcg atttcgccga
     7741 ggcgctggtt ggttagcttc tccgcggtcg ccaagcgcta ccccggcggc ctgcaagcgc
     7801 tcaaagacgt cagcttctcg ctcgaggcgg gggagctcgc gttcctcacc gggcgctcgg
     7861 gcgcgggcaa gtcgacgctt ctcaagctga tccccgcgat cgagcggccg agcgccggct
     7921 cggtggtggt gaacgggcag aacgtcagcg cgctcaagcg ctccgccatc ccctacctgc
     7981 ggcgcaatat cggcctcgtg ttccaggacc agaagcttct ctacgaccgc agcgcctacg
     8041 acaacgtgat cctgccgctc gccttctcgt cgcatccgcc gaaggaagcg gcgcgccgcg
     8101 cacgagccgc gctcgacaaa gtcggcttgc tcggaagaga gaaatcgaac cctgtccagc
     8161 tctccggcgg cgagcagcag cgcctcgcga tcgcgcgcgc ggtggtcaac cggcccgcgc
     8221 tgctcgttgc cgacgagccg accgcgaacc tcgacgcgga atcggcggcg cgcatcctcg
     8281 agatcttcgt cgcgttcaac caggtcgggg tcacggtgct catcgccacg cacgaccaga
     8341 cgctcgtcgc gcgctatggc aaaaaggtcc tgcacctgga cgcaggccgc ctcgcgtgag
     8401 cgcctggctg cgccagcacc ggcaggcctt cgccgcagcg tgccgaagac tgggctggtt
     8461 gaacgccctg gtcatcggca tcgcactcgc cctgcccgcg ggcggctatg cgctcctcga
     8521 gagccttcgc ccggcgggcg cgcggctcgc gctcgagccg cgaatgacgc ttttcctcga
     8581 cgcgcaggtg cgccgcgcgg acgccgaagc gctcggcaag cggctacgcg ccgatccgcg
     8641 catcgcgggc gtgcgcttcg tcccgcgcga ggaggcgctg aaggagatga gcgcggtcca
     8701 ggggctgtcg gaggtgatcg cggccctcgg ccgcaatccg ctccccgacg ccctggtcgt
     8761 cacgtcgcgg gagaacatcg ccgacgagct cgcccggctg ccgggcgtcg cgcacgtgca
     8821 ggccgatgcc gtctgggcac ggcgcctcgc cgccgccgcc gcgatcgtgg agctcgggct
     8881 gtggctgctg gcggcgcttc tgggcgcggg actggttgcc gtgaccttca acacgatccg
     8941 cctccagatc ctcacgcagc gcgatgagat cgaggtctcg aagctgatcg gcgcgaccga
     9001 cgctttcatc cgccgcccct tctactacct cgggctcttg caaggcctcg tcggcggcgc
     9061 cgtggcgctc gcgctcgtcg tggcggcgct cgcccttctc aaccgcgagg tgagcgtgct
     9121 ggccgagtcg tacggttcga gctttcgcct gggctttccg gctgaggggg cgctggctgt
     9181 cgtggcgttc gccgccttgc tcgggtggct aggcgctcag ctctcggtga gccggcacct
     9241 tcgcgcgata gagccaatgt gagcgcctac tgtcaacata agttcatgaa gttacatatg
     9301 aaatatcggg aacccccgcc accattagca ctctaataaa tggactgcta aaatgcccga
     9361 tcaaggagga atgagcccta tgcaagcgac gatgccccta ctcaggtcgg ttccgatgcc
     9421 gatgccaacg gggagcctcg aggcttacat ccagaccgtc aaccgcatcc ccatgctgac
     9481 gggaccggag gagcgtgagc tggcgctgcg cttcagggac cacaacgacc tcgaagcggc
     9541 gcgccagctc gtgctttccc acctgcggct ggtggtcgcg gtggcgcgcg gctacctcgg
     9601 ctacggcctg ccgcacgccg acctgatcca ggaaggctcg atcggcctga tgaaggccgt
     9661 gaagcgcttc gacccggacc gcaacgtgcg gctggtgtcc ttcgccatcc actgggtcaa
     9721 ggccgagatc cacgaataca tcctgcgcaa ctggcggctg gtcaagctcg ccacgaccaa
     9781 ggcgcagagg aagctcttct tcaacctgcg ctcgatgaag agcggcctcg ccccgatgac
     9841 cgtcaaggac gtcgagcgca tcgccggcga gctgcgcgtc aagccggagg aagtggtcga
     9901 gatggagacc cggctatcgg gccaggagat cgcgttcgag gccgaccagg acgacgagga
     9961 cggctacgct cctgcgaact atctcgccgc cgatcgcgac gccgagccgc tgtcgcagct
    10021 cgaggccaag gaatccgagg aaggccgcgc cgccgggctc gaagacgccc tgcagagcct
    10081 cgacccccgc agccgccgca tcatcgagac gcgctggctg cgcgagaagg acgccctcac
    10141 cctccacgac ctcgccgcgg agttcaaggt gtcggccgag cgcatccgcc agatcgaggc
    10201 gaaggcgctc gagaagatga agaaggtcat gcagtccgag cccggcgcgc cgctcgcgct
    10261 gccgtcaccc acctagcccg gattcttcag cagggttcgc aggtccggcg cgaggtcttg
    10321 cccgatgcgg tcgtggcgca cgagcaggcg caaccggccc tgcgggtcga tgacgtagct
    10381 ctgcgcgctg tggtcgacgc tgtagctgtc gccctgcttc cgcttctcga agtagatctt
    10441 gaactcggcc gccacctttc gcgtggcggc caggtcgccg cgcaggccga ggaagcgcgc
    10501 gtcgaaggca gtcacgtatt tcgcgagcga gtcctgcgta tcgcgctccg ggtcgacgct
    10561 gaccatgagc agctgcacgc gctccgcgtc gggccccagg gacctgatcg cctgggaaat
    10621 ctccgccagc gtggtcgggc agatgtccgg gcagtgggta aagccgaaga agagcaccac
    10681 cgccttgccg cggaaatcct ccagccgccg ggctcgtccg gtgtggtcgg tcagctcgag
    10741 cgactggccg tagggcgcgc cggtgatgtc ggtgctcttg aattgcgggc ggctgtcgca
    10801 gcccgcaatc gcgctacaga tagtgatcga ccagaagagc cgcgaaaagg aacgcaaggt
    10861 aggcgatcga ataacggaat gtctttcgcg ccagggcatc gctgtaggcg aaatagatgc
    10921 gcagcgcata ggccagaaaa accgcgccga gcgcagccgc ggcgatcagg tacgcgaggc
    10981 cgctcatgcc cgcgacgtag ggcagcaggc tcaccgcgaa caggatcagc gtatagagca
    11041 gcacctgcag ctgggtgtat tttcgcccgt gcgtgaccgg cagcatcggc acgccggcgc
    11101 gcgcgtagtc ctcggtgcgg tatagcgcga gcgcccagaa gtgcggcggg gtccaggcga
    11161 aaatgatgag gaacaagagc atcgcctcga tcggcacctc gccggtcacc gccgcccagc
    11221 ccagcaccgg cggcatcgcg cccgaggcgc cgccgatcac gatgttctgc ggcgtcgccg
    11281 gcttcaagat cacggtgtac acgatggcgt agccgacgaa ggtggcgagc gtcagccaca
    11341 tggtgagcgc gttgacgaag ttgtagagca gccacaggcc cgcgccgccc agcgcgccgg
    11401 cgaacaccag cgtgtgcgcg gcggaaagct cgccttgcgg cagcgggcgc cagcgcgtgc
    11461 ggctcatcac cgcgtcgatc ttctgctcga ccaggcagtt cacggccgcg gccgcccctg
    11521 cgacgagagc aatcccgatc gtgcctgcca ggagcgcctg catcggcacc acccccggcg
    11581 tcgcgaggaa catgccgatc accgcggtaa acacgatgag cgagactacg cgcggcttgg
    11641 tgagggcagc gaaggcacga aagcgcgcgc gcagcttctg cgcaaagcta tgggaaagaa
    11701 cttgtgtgtt cacggaaagc aaaaaagttt agcacggcga gggccacgct tcatttggtg
    11761 cgcggcgaat acttcagcag ccgctgcagg tctttgatca tcttcgacgg gtccgggtcg
    11821 cgcgggaagc gcagcatcag gttgccaaac ggatcgacca ggtagatgtg atccgtggct
    11881 ttgcccggaa agctttccga tgaccatgcg gcaaggcgcg tcccttcgat cccgggcagc
    11941 aattcggcgg gcggcttgcc gccgtcggtt acgacccaga tgcgctcgac acggtccatc
    12001 tccttgccct gggcgcggcg cacctggcgc atgaaataga gcttcttctc gcaatacgcg
    12061 tcgcactcgg cggcgtcgaa gctgacgagc acccatttgc cgcgcagcgg gtcgaacccc
    12121 gaaacgggcc tggggtccag cagttcgccg tagttggtcg cgccgccggg cgaccagccg
    12181 agcaaatagg ccgcggtgcc gagcacgatc ggcgcggcac agatcagcgc gacccagagg
    12241 agcttatttc gaaggaacgt caattttccg gaacgacagc acgacggcga ggatcagcgc
    12301 aagcgccgcc agcgagtacc actgcagcgc atagccttcg ttcttctcga cgccggcatc
    12361 gtgcggcggc cagtcgcgcg cgaggccgtc gggcaggtcc gagtgctgct cgatgacgcg
    12421 cggctcgagc cgcaggccgg tctctgccgc atacgccgcg atatcgaggc tctgacgcac
    12481 acgtgacttg tcctctttca gcttcagggg ttgcgccagc cgttccaggg caaggccttc
    12541 gagccgcagc tctcccgccg gcgtgcgcac ctcgggcagg acttcgcgcg tcttgcccgc
    12601 ctcgatccag ccgcggttga ccagcacgtg gctggaggac ccgcgaatct tcatcggcgt
    12661 gacgatctcg tagcctgcct tgccgcgacg cgtcttgttg tcgagataca ccgtatagcg
    12721 cggcaggaac tcgccctcga ccgccacgcg gggaagtact actcgctttt cgtctgcgcg
    12781 gcgcaattgc cagttcccca gcgcgatgcc cgcggcgcac gccgcggccg cgagcgcgag
    12841 cgcccagagc cgcggacgga aagagtatcc tgccggcatg cgctacgtgg tgatcgccat
    12901 gctggtcgcg atcgtcgcca gcctcggctc ggcgctctgg tacatgctgc gccccgggaa
    12961 ggacccgaag cggatggtca aggcgttggc gctgcgggtc gggctttcgg tgaccttgtt
    13021 cgtgttgctg atggccggct actactttgg cttcattacc ggtcgtctat aaccagtaga
    13081 cgaagataaa gaggccgagc cagaccacgt cgacgaagtg ccagtaccag gccacgccct
    13141 cgaacgcgaa gtgatggtcg ggcgtgaagt gccccttcag gcagcggaac cagatcaccg
    13201 tcagcatgat cgcgccgatg gtgacgtgga atccatggaa gccggtcagc atgaagaagg
    13261 tcgagccgta gacgccggtg tgcagcgtta gattcagctc ctggaaggcg tgcacatatt
    13321 cgatcgcctg gcagacgagg aaccccacgc cgagcagcac ggtcgcgccg agccaccaca
    13381 agagcttggg gcggttgttg gcgatcaggt tccagtgcgc gaacgtcacc gtcacgcctg
    13441 aagagaggag caggatcgtg ttcaccgccg gcaggcccca ggcgcccatc ggcgtgaact
    13501 tctcgatgaa cgccgggccg gcgttcggcc agtgcgcggt gtagccgggc cacatcaggg
    13561 cgttgctttc gatgctcgcg aggtcgggca ccgagatcac gcgcgcccag aacagcgccc
    13621 cgaagaacgc cgcgaagaac atcacttccg agaaaataaa gaagctcatt ccccagcgga
    13681 acgagacgtc ttcccagcgg ccgtacttgc cgccctcgga ctcgctgatc acctcgccga
    13741 accagcgcac catcatgtag agcagcagca tgaagccggc ggctatcccg atccagccgc
    13801 cgctcatgcc gttgaacacg aacaccgcgc cgagcgccat catgaacagc gacgccgatc
    13861 ccatgaccgg ccagggcatc ggctgcggca ccgagtagtg cgcctgcttg ccgtgggggg
    13921 tggatgcgct gtgcgccata ttggttctct ctccctagct ggtcacgatc cgcacgatcg
    13981 tcaccaaagt cagtatgaac agaacgacga agccgatcgc ggcgatcgcg agatggaccg
    14041 ggttgagcgg ggtcttctcg tgatcggcct tgcgccggat gccgaccatg ccccacagga
    14101 cggtcttcac cgtctctatc acgtctttcc tccgcggccg gcgacttcga agaaggtgta
    14161 ggacagcgcg atggtgttga cgtccttcgg cagcttcggg tcgaccatga acactaccgg
    14221 catgcgccgc gtctcgcccg ccgccagcgt ctggtgggtg aagcagaagc actcgatctt
    14281 cctgaagtac tcgccggcgc tctgcggccc gtagctcggc accgcctgcc ccgtcaccgc
    14341 cgcctcgcgc acgttgacga tctcgtactc gaccatcgcg agctcgcccg ggtgcacctt
    14401 cacctcggac accagcgccc tgaagcgcca tggcaggccg tgcacgttcg agtcgagctc
    14461 gatcgtcacg gtgcgcgaag tgtcgacctg ggtattgagc gcttcggccg gttgatcgag
    14521 gttgttgatg ccgagcgcga cgcagatctg gttgtagaac ggcaccaggg cgaaggcgaa
    14581 gccgaacatg gcgatcgcga cgatcgccag gcgacgcgac agcctgcggt tctcccgttt
    14641 cgcgttcacc gcagcagcca atacttcgcg atgatgccca ggaaaaacgc cagggcgatc
    14701 gacgcgagga tcagggccgt ctttgcgttc ttgctcactc actgggtcat ttcacttgat
    14761 cgtgggctgg gtctcgaacg tgtggtaggg cggcggcgtc ggcaggtgcg tccactcgag
    14821 cgagtcggcg ccctcccagg gccgctgcgg cgcgggctcg ccgctgcgga tggtcttcac
    14881 cacgatgtag aggaacagga gctgcgcgag gcccatgccg aaggcgccga gcgaggagat
    14941 cttgttccac agctcgaaca tcgggtggta gtcgggaatg cgccggggca tgcccgccat
    15001 gccgaggaag tgctgcacga agaagatcat gttgaagaag atgagcgtca gccagaagtg
    15061 gatctggccc agccgctcgt tgtacatgcg cccggtccac ttcggcagcc agaagtacac
    15121 cccggcaaac gccgcgaaca gggcgccagc caccatgacg tagtggaagt gcgccaccac
    15181 gtagtaggtg tcgtgcatct ggatatcgat cggcaccacg gcgagcacca gtccggtgaa
    15241 gccgccgagc gtgaacatga acaggaagcc gagcgcgaac agcatcgggg tctcgaaggt
    15301 gagcgagccc ttccacatgg tcgccaccca gttgaacacc ttcaccccgg tgggcaccgc
    15361 gatgatcatg gtcgcgtaca tgaagaacag ctgcccggtc accggcatgc cgaccgtgta
    15421 catgtggtgc gcccagacga tgaacgagat gatcgcgatt gccgaggtgg cgtagaccat
    15481 cgaggcatag ccgaacaggg gcttcctcga gaacgccggg atcacctgcg agatcacgcc
    15541 gaaccccggc agcgcgatga tgtacacctc gggatggccg aagaaccaga agatgtgctg
    15601 gtacagcacc gggtcgccgc cgccggcggc gttgaagaag gtagtgccga agtgccggtc
    15661 ggtaagcgtc atggtgaccg cgccggcgag caccggcatc gccgccacca gcaggtaggc
    15721 ggtgatcagc caggtccaaa cgaacagcgg cattctcatc agcgtcatgc ccggcgcgcg
    15781 catgttgagg atggtggtga tgatgttgat cgagcccagg atcgaactca tccccaggat
    15841 gtgcaccgcg aagatcgtca ggtccatgcc gatgcccatc tgcacggtga gcggcgcgta
    15901 catcgtccag cccacgcccg gcgctccgcc cggcatgaag aacgacagca cgagcagcgt
    15961 ggcggcgaac ggcagcagcc agaacgacca gttgttgagg cgcgcgaacg ccatgtcggg
    16021 cgcgccgatc atcatcggga tctgccagtt cgcgaagccg acgaacaccg gcatgatcgc
    16081 gccgaacacc atgatcaggc cgtgcatggt ggtgagctgg ttgaagaagt ccgggtccat
    16141 gtgctgcagg cccggcgaga acagctccag gcggatgccg accgcgagca gcccgccgat
    16201 gctgaacatg atgaagctga accacaggta cagcgtgccg atgtccttgt ggttggtggt
    16261 ggtgacccag cgcatcagcc ccgcggcggg accgtggtgg ccgtgatcgt cgtgggcgtg
    16321 accgtgatcg tggacgactg cgctcatgag cgttatttcc ttctagcctt gacttcggcg
    16381 ggctggacgt cgctcgcctt gtttccccag gaattgcgcg cgtaggagat caccgcggca
    16441 atctcggtat cggagagttg ctgcttgaac gcctgcatcg cggtgttcgg ccggccgttc
    16501 agcaccgtgt cgatcggccc cggctcggcg ccggtgacga acttgttgcc ggccagcgcc
    16561 ggcgccttca tcgccgccaa tccctggccg gacgcctggt ggcaggccac gcagttcgcg
    16621 gcgtagacct tctcgccgcg cgcgaccagc tccttcaggt cccaggcctt gttcggatca
    16681 tcggcggccg cggccatcga cttcttctgg ctttccaccc acttgctgta ctcgggcgcc
    16741 gaaaccacgc gcaccacgat cggcatgaag ccgtgctcct tgccgcacag ctcgacgcac
    16801 tggctgcgga aggtgccttc ccggtcggcg cggaaccaga ggtcgcggat gaagccgggg
    16861 atcgcgtcct gcttggcgcc gaacgccggc acccaccagg cgtgcaccac gtcggcggcg
    16921 gtggtcagca cgcgcacttt cttgcccacc ggcaccacca cttcgttgtc gacttccagc
    16981 aggtagttct cgcccttggg cgccttgccc tcgatctgct cgcgcggcgt ggcgagcgtg
    17041 gagacgaagc tgatgccctc gccttcgccc ttcaggtagt cgtagcccca cttccactgg
    17101 tagccggtca ccttgatggt gaggtcgggg gcggaagtgt ccttctgcgc gatcaccacc
    17161 ttggtcaccg gccaggcgat cacgatcagg atcagcgccg gaatcacggt ccagagaatc
    17221 tcgaccgtgg tgttctcgtg gaactgcgcg gcccggtggc ccttggattt gcggtgcgcg
    17281 aagcaggcgt agaacatgaa gccgaacacg ccgatgaaga tcaccaggat gatccacatc
    17341 agcacctggt gcagccagtc gatctcgcgc gccatggccg aggccggcga ctggaagtac
    17401 caggcgatgg cgtgcgccgc gctcgatgcc gcgagcgcga tcattccagc caaggccgag
    17461 aaaagcttcg atcccttcat gtagcgattc ccctgatcaa ccttttctgg agctcggcgg
    17521 ccagctcgac gcgcgcttcg gcgccgaggt aacggccgac ctctagttct tctttcgcgt
    17581 cgcgcaacac gacgcgcccg tcttccaccg acaccttcgc attcaccagc ctgtagcgcg
    17641 cgatgccctg cgccttcgcc acctcgacgc tgacgccgcc cggctcgatc tcgaggcgct
    17701 cgtagtccgc cgcgtgccgc gcgtacgcca cgtacgcgcc gccgagcagc aacacttcga
    17761 gccccgcgaa cggcaacacc agccaggccc ccgccagcgc gaaaccggcg ccaatcagca
    17821 gcactaccag cgccagcgcc gcgaacaccc ccgccagagc cttcggcgac atcgagcaat
    17881 tgcgcttcag gaccaggctg aaggaaggcg cgctcacgga cacgcgaaag tatcacatcc
    17941 gggaagcgcg cctcaaggca tgcgcttgaa aatacgcacg gttttcgggt cctggttcgc
    18001 gtcgcgggtc gccttcgcgg tccgccaggc atggccgtac acgacctcgt aggtggcgcg
    18061 cggcggcagc gcgcgcaaac gctcgccgaa gcccttgccc gccagccccc gcggccggtc
    18121 ggcgcgcgcg ttgatttgac ctgtgttctt caaatcctcg accaatcccc ccgctgcggc
    18181 gtaagcgatc tggagcatct ccatgtccat caccggggcg gaaaacccgt tcgccaccag
    18241 catgtcgccc aggtcgtgca tgtcgatgaa cgcgtgcacc cgcgcggcgc cggcggcggc
    18301 gcgcagttcc ttcagcgtgt ctggtccgag cgtgctgaac atgagcagtc cttcgggggt
    18361 gagcacgcgc gcaaactccg cgaacgcgcg ctgcggatcc ggcagccagt gcagcgccat
    18421 gttgcaccag acgaaatcga cggcgccgtc ggccagcggc aggcgctcga gatcgccgca
    18481 tacccgcagc gagttcttgc caaagatcct cttcctcgtc gcgcgaagca ttgcctcggc
    18541 aaagtccagc gcaatcacct gcgcaccggg gtagcgccgc ccaagctcgc ggcgcggcgg
    18601 gccgctcgcc gcgtcgagga tgcgccacgg cgcgagcttc atgtagtcca ggcgctcgag
    18661 catgcgcgcg gcgacctcgc cttcgagccg cgaggccgcg ccgtaggtgc gcgcggcgcg
    18721 ctcgaagcgc cggcgcgcga ggtcccggtc aatcccgggc gagcgtgcgc tcaatcgccg
    18781 cggccaccgc caggatgcgc cggtcgctca tcgccgggcc ggacaccatg agcccgaccg
    18841 gtgccgcacc tgccgcctgg cagggcagcg agaccgcgca accgtcgagg aaattcacca
    18901 ggccgacgat gcccaggatg cgcatgttcc agcggaagta ggactcgtcg ctcgcgttga
    18961 cctcggcgat cggcggcggg acgcagggcg tggtcggcag cagaaacgcg tcgaacggcg
    19021 ccagcgccgc ctcgacttcg cgctggtacg ccgtgcgcag cagcgtgagc tcgacgaaat
    19081 ccgccgcgct caagtccttg ccgagcagca cgcgcttggc gacgcgcggg tcgtactcgc
    19141 tcaggcgatc ggcgttcttg cggtggatgg cgtaggcctc ggcgccggcg aagccgccgc
    19201 ccttgaaata ttcgctctgc cggtcgaacg ccggcagcgg cttttcggtc acgacggcac
    19261 cggcgcgctt caatcgttca acggccgaat tgaatgcttc cgccacttcg cggtccaggc
    19321 cctccatcac cgcgctcttc ggcaccagca atctcaatcc ccgagcgggt aactccgcga
    19381 gcgcatccgg cgcctcgccg gcgaggatcg cgtcgtacgc cgcgcagcag gccaccgagt
    19441 tggcgagcgg cccgatcgag tcgagcgtgt aggagagcgg gaacgcgccc tcgcgcggca
    19501 cgcgccgcgt ggtcggcttg aagccggtca cgccgcagaa cgccgccggg atgcggaccg
    19561 agccgcgcgt gtccgagccg agcgccatca cgcacatgcc gtcggcctgc gccaccgcgg
    19621 cgccggacga cgagccgccg gggacccggc ccgtaagcgg ctcggccgcg ttccatttcg
    19681 ggcgatccca cgggcttcgc ggcgtgccgt aatgcggatt gatgccggtg gtgccgaacg
    19741 cgaactccac catattggta cgcccgacga acaccgcgcc cgccgcgcgc aagcgggcga
    19801 ccgccgccgc atcgctttcc gccttctgat ggtgaacctt cgagccggcg cgggtgacat
    19861 cgccggccac gtcgaacagg tccttgagcg aaaccggcag gccatccacc ggcgagcgcc
    19921 gcacgccgct cttgcgcagg cggtcggccg tgtcggcgtc ggccagcgcg ctgtcggcgt
    19981 acaccttgag gaaagcgcgg ccgccctcgc cggccggatc ggcgatgcgc gcgagcgcct
    20041 gctcggtcag gcgccggctg gtggtgcgtc ccgcggccag atcggcggcg agctgcgcta
    20101 cagtccacat ggggccaagt gtatgcggaa tttcttcgcg cagttgtttg gcggcagctg
    20161 ctttctgtgc cgcggcgcgg ccacggatct gctctgccca tcctgcgacg ccgacctgcc
    20221 gcgcctcgcc gcgccgtgct gcccgcgctg cgcgctgccc tcgccccggg gcgaagtctg
    20281 cggccgctgc ctgcatgagg cgccgcacta cgacgccacc atcgccgcgc tcgcctatgc
    20341 gtttcccgcc gacgtgctgg tgcacgcgct caagttccgc ggcgagctcg cgctggcagg
    20401 cttgctgggc ggcctgcttt cgcgaaaaat ttcccaccag gtggattgca tcgtccccgt
    20461 gcccctttcc tccgcgcgcc tgcgggagcg cggctacaac cagtcggtcg agatcgcgcg
    20521 ccagctcggc gcgcagctcg acctcgcgct gtgcgtgcgc gagcgcgaca cgccggcgca
    20581 gatggacctg ccctacgcgg agcgcaagcg caatgtgcgc ggcgcgttcc gctgcacgcg
    20641 ctcacttggc ggcactcgcg tggccgtggt cgacgacgtc atgaccaccg gtgccacgct
    20701 gaacgaagtg gcgtcggtgc tgaagcaggc cggcgcggcg caggtcgtca actgggtcgt
    20761 ggcccgcacg ctgcccgatg tttaacatcg tcctggtcca gcccgagatc ccgcccaacg
    20821 ccggcaacgt gatccgcctg gcggcgaaca ccggcgcgcg cctgcatctc gtcgagccgc
    20881 tcggcttcac gctcgaggac cggcagctca agcgcgccgg gctcgactac cacgacctgg
    20941 cgcacgtgac ggtgcaccgg gactgggaaa cctgccgcgc cgccctcggc gagcggcgcc
    21001 tgttcgcgct ctcgacgcgc ggcacgcgcg gctacgccga ggtcgagttc cgcgtcggcg
    21061 atacgttcgt gttcggcgcc gagacgcgcg gactgtccgc gcagctgctc gacgaattcc
    21121 ccgccgccat gcgcctgcgg ctgccgatgc tcgccggcaa ccgcagcctg aacctgtcga
    21181 acgcggtggc ggtggtcgtg ttcgaagcct ggcgccagat cggctttctc ggctccgcct
    21241 agtcagaacc gcgtgctgac gcccagcacg ccggcccagt ccgccgtaag ctgcacgccg
    21301 ttcacgtact ggtaaagcgg cagctgcacg aacgcgtaag cctgcacgcg cttcgacatc
    21361 atgtagctga tgccggggct cacgtatagg aacgtgccgc cgctgttagc gggctcggct
    21421 tcgcttccct tgtcccgatc cttgtaaagc aggttgagct gcagcatgcc cgcgagcacc
    21481 gaacccaact cgcgccggta gcccagatcg agcgagacgc gcgagcccgg ctcgtagttg
    21541 tcacgcgcat cgaacgggct ctgcactagc gcatcggcaa accaggacga gccggagccg
    21601 agcacgcgcg tgaagtagcc gcccaccaac agatcggtcg tgccggatcc cggctgcaga
    21661 ctgcgctcgg cgagggcgcc ctcgtggtta cgcacgtcgc gcttgccggt cggcaccttg
    21721 agcccgaagt tcacgccgta gaagtccacg cgctcggcgg ccgggctttc ggagcgccac
    21781 tgccgccggc cgagcatgcg cgcatcgccg atctcccgga agtcccagga ttcggtgagc
    21841 tgcgcgccgt ggtggttgtg gatatgggtg tgatcgcgat cgatcagcgg cagcgtcgcc
    21901 gatatccccc agtccgcatt gaaggcgtag tccactgtcg ccagccagtt gcggttgatg
    21961 gtgcgcacct cgtcgtggtg ctggctgatc tggccgacgc cgactttgct gtcgccggcc
    22021 atcggctggt cctggtcgat atactcgaag cgcaggtcga agcgcacacc gggctcggtc
    22081 cagacgccct gcgtgttcca gctggtattc accatgcaga aggcgctgcc gcatgaggcg
    22141 tgcgcgccgc tcgcgaacgt gcatgcgcag gcgagcgctg ccaataaccg tcgtttcaca
    22201 ttcttctccc tgtcgacaac tagaaaaaag ctagctgcgt acgatcagga
//
LOCUS       DATBSZ010000255        32885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1209456, whole genome shotgun sequence.
ACCESSION   DATBSZ010000255 DATBSZ010000000
VERSION     DATBSZ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 32885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32885
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209456"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VIG70_16280"
     CDS             complement(<1..417)
                     /locus_tag="VIG70_16280"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8252156.1"
                     /translation="MRILVTGAAGYIGSALVKALAGRDVLATDQSSPAPVIGNIAYPQ
                     FARSLITPDIGSVFHLASLVSGGAEQNFELGTKVNLDATRDLLEACRLAGHCPKFVFA
                     SSIAVYGGVDEVTDETPVAPRLSYGAQKLVCEVLIDD"
     gene            451..1407
                     /locus_tag="VIG70_16285"
     CDS             451..1407
                     /locus_tag="VIG70_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8252157.1"
                     /translation="MVRTLLALLLLSFGSAAWTQSYPSKPVRIIVPFGVGGSADVYAR
                     YLGAKLQDSVGQSFVVENRPGGGSIVGSDVVAKSDPDGYTLLMMSNTHTVNETLIPKK
                     PFELMRDFAPITGVSYSDLIMVVHPSVQAASLKEFIALAKSKPGALNYASSGNGTPYH
                     MAGELFKSMAGVDIVHVPHKGSDQARVAVMGGQVQMMFDAVPTMAANAKAGKVKAFGT
                     TGEKRSSVTPELPTLSEAGVAGYETGLWLGLMAPAGTPRPILERLNSEVNKLLNSAEV
                     KQNWAKQGTITMGMSIDQFDKFLRQDIQKWSKLVKSTGMKVD"
     gene            1404..2018
                     /locus_tag="VIG70_16290"
     CDS             1404..2018
                     /locus_tag="VIG70_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8252158.1"
                     /translation="MTNEQLAIVEVVHNWALWRDAGDWQRFASVWHDDGWMTATWFQG
                     PAQEFIKVSKAGFEKGVSILHFLGGSSVDVNRTRAIAQTKMTISQRGPCEGELVDVVC
                     TGRFYDFFEKRAGRWAIVRRQPIYEKDRMDPVDPAVRIKLDPDLLGSIPEGYRHLGYL
                     QSKLGFQIKRDLPQLKGAAVELLYRQGRAWLDGAASAFPDEERP"
     gene            2077..2688
                     /locus_tag="VIG70_16295"
     CDS             2077..2688
                     /locus_tag="VIG70_16295"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY8252159.1"
                     /translation="MTPAAQPFEVVVARLDAPSDELRALRESLCAAEQHRAGRFRFER
                     HRRRFVVARGRLRKLLGERLGVPAEKVELAHGANGKPCLKHNGWHFNVSHCDDVALFA
                     FSKAFEIGVDVEAIRPIRGADTIAAQFFSPREKAAYAALAPDDKALGFLHCWTRKEAL
                     VKAIGEGLSMPLERFDVAHAPGWRLHSFFPLPGFIGAVACQHG"
     gene            2681..3859
                     /locus_tag="VIG70_16300"
     CDS             2681..3859
                     /locus_tag="VIG70_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8252160.1"
                     /translation="MDRLQIPLTAYESPWVNDDVRMFRKTVSQFIEKEFVPHQPRWRE
                     NRGPDPESWANAGRAGILLADLPEEYGGGGGTFAHEAVVLEELARAGVHFGSAIQSTV
                     AHYILGYGSEEQKRKWIPRLARGESVAAIAMSEPGAGSDLQAIKTSARRDGEHYVING
                     SKTFITNGARADLVCLAAKTNPKAIGMRAISLIMVETKDLPGYRVGRALEKVGMHGQD
                     TVELFFDDVRVPVSNLIGGASVANEGRGFSQMMEQLPYERLQIAVGAVAMAEQAVAIT
                     ARYVKERKAFGRPLLDLQNTRFKLAECKTNAQVGRVFLDNCIERFIAGRLDEPSTAMA
                     KYWLTESQCRIVDECLQLHGGYGYMTEYPIARMWADARVQRIYAGSNEIMKELIAWSL
                     "
     gene            3847..5562
                     /locus_tag="VIG70_16305"
     CDS             3847..5562
                     /locus_tag="VIG70_16305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEY8252161.1"
                     /translation="MVALKLCLHELIEAQAARTPNAPALAFEGQALTYRELDLRARRL
                     AAHLAGLGVGPDVTVGLFVERSLEMIVGILGILKAGGAYVPIDPAYPAERVAYMLSDS
                     GAAAVLTQSSLADKVSGVQAVCLDAFAWNKDVPRIEAPVQSRHLAYVIYTSGSTGQPK
                     GVGIEHRNIVNYVLGVIERLQLEPGMRYAVVSTIAADLGNTVIFPSLATGGCLHVISQ
                     ERAENGGLLAEYFEREKIDVLKVVPSHFAALNPEGVMPKRRLILGGEASRLDWIERLR
                     ALAPACEIFNHYGPTETTVGVLTCHAAGRIPATQSGSLPLGKPLPNVEIRLVDGEICI
                     GGAGVARGYLNRPELTAEKFVDGFYRTGDRGRELADGSIEFCGRIDHQVKVHGYRVEL
                     GEIEAALRSHEKVRDAVVLLREDQLVAFVAPSEADARTLREHLKARLPQYMVPSVVAP
                     MERLPLTPNGKVDRQALARFTVQKETPAAAPRTETEKALAEIWGELLQAEVGVDEDVF
                     DLGAHSLMAMKALTQIRDRFQVNLSLRNLFEHPTVAGLAGVLDGVWLTQARSPASGAQ
                     EEFVL"
     gene            5559..6536
                     /locus_tag="VIG70_16310"
     CDS             5559..6536
                     /locus_tag="VIG70_16310"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEY8252162.1"
                     /translation="MNAATFLSELRRREIQVRAVGDQLHFTAKAGALTPELREQLRAW
                     KNDILKLLASAQALDTQERAIVPLQAKGTRVPVFAVPGHNGDVFCYRALAQRLGEDQP
                     FYGLQPPGVDGKDEPLGRVEDLAASFARQIRVFQPKGPYVIAGYCAGGTVAFELAQQL
                     SRDGAAVNLLALFGSPYPAYFRLPAQLLQRVAEHAARIGGLARDLAVRSPAQYRRYFA
                     ERMRRHRDRVSAAAAAAADPVLALRARVERATVAAVRAYTPGDFDGRVALFLPSMEWD
                     LAQRWRSVAPHAEQYFGPDACSSQDMLREPNATIFAELFAAAVQRWKMP"
     gene            6521..6673
                     /locus_tag="VIG70_16315"
     CDS             6521..6673
                     /locus_tag="VIG70_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEY8252163.1"
                     /translation="MENALIWFVAFLGLAIAGLGGLFLYGWRRRKEKPPPGVKPLRDE
                     DDDWGR"
     gene            6675..6866
                     /locus_tag="VIG70_16320"
     CDS             6675..6866
                     /locus_tag="VIG70_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252164.1"
                     /translation="MTKKQGRRADDISAEEIKQFWHGFCERKGVDAATRARGDAKIDQ
                     DPEHWADQTMLKLLDAVAI"
     gene            6945..7232
                     /locus_tag="VIG70_16325"
     CDS             6945..7232
                     /locus_tag="VIG70_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252165.1"
                     /translation="MEKIEIPADNCPVAPPRRITVHTRVLHRACQHLGGVEHLARHLK
                     VSPDLLHSWLEGEDVPPSSIFLKAVDLVLPAWGPDDDALAKTISASRPKKN"
     gene            complement(7331..8518)
                     /locus_tag="VIG70_16330"
     CDS             complement(7331..8518)
                     /locus_tag="VIG70_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEY8252166.1"
                     /translation="MKFGIDRLLAERALAKRLAGRRVALLAHPASVTQDLEHALDALA
                     AAGVKLTAALGPQHGLRGDKQDNMVESSDFTDPVHGIPVFSLYGAVRRPSAQMMERFD
                     VLLVDLQDLGCRVYTFLTTLRYVLEAAAQHGKSVWVLDRPNPIGRLVEGLKLRPGWES
                     FVGAGALPMRHGLTLGEAAKWFVRSAKIDVDCEVVEMKGWKPAEGWPLGERAWVNPSP
                     NAPNLGMARCYPGTVMLEGTTLSEGRGTTRPLEVLGSPDLDSAAILKKMHSLEPRWLR
                     GCRLRPCWFEPTFHKHAGKLCAGLQIHAEAPHYGHAAFRPWRIVALALKAIRSLAPEY
                     PLWRDFPYEYETRLAIDVINGGSLLRQWVDDAGAKPGDLDAAARADEDAWREEREAVL
                     LYH"
     gene            complement(8515..9030)
                     /locus_tag="VIG70_16335"
     CDS             complement(8515..9030)
                     /locus_tag="VIG70_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HEY8252167.1"
                     /translation="MPSAALERHPATSCAALRGIKVSLTRKPRRMQVAYVLDGEIDRL
                     RIPPPRPPRVAEGLWQHTCCELFVARAGEPGYREFNFSPSGEWAAYAFEGYREGGRSL
                     DVPSEIVVRQGAGRLELAASIPFPETQKMLIGLSAVIEEESGALSYWALRHAPGKPDF
                     HHAQAFALALG"
     gene            9048..9578
                     /locus_tag="VIG70_16340"
     CDS             9048..9578
                     /locus_tag="VIG70_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252168.1"
                     /translation="MGIVKGLGTLAVAALVGWAFLAPPSGSWAFAAAEFAFLAWLARE
                     VRKADAAALRARALEPLEPDEADIVQRYPLYFARPALARECASTLSALGLASLVVVPW
                     LTYKVQWAPAILIGVFLFAVARLTKLLSPVYALRLATAKGDRKALRLLSAHDGAARKL
                     SAHHVPQPEEHRAAGR"
     gene            complement(9528..9758)
                     /locus_tag="VIG70_16345"
     CDS             complement(9528..9758)
                     /locus_tag="VIG70_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252169.1"
                     /translation="MAPWLEGALVGLVFGAILVGYEYYAVNKQVVERAAARHLKPEWE
                     PQDRNRVMSVLRFAIFLPAGGAVLFWLWNMMG"
     gene            complement(9770..10501)
                     /locus_tag="VIG70_16350"
     CDS             complement(9770..10501)
                     /locus_tag="VIG70_16350"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY8252170.1"
                     /translation="MRRLSSRSYMRSRKHIIFGVLGLVAAALALNWVYQVVRKPAELF
                     FPVSGALFKTPTETWQHYEPIFRRHSTAVMTPELLAALAQVEGSGNPVARTPWRLRAS
                     SDPREVYRPASSAVGMYQITDGTFREAKRYCIRDNAVLEDGCWFNSLYMRVIPSHAVE
                     LTSAYLDRRVAMVLERHRLKATLEQKQDLAAIIHLCGAAAGDSYARRGFRLAPGQRCG
                     EHDPRAYLARVNAMKRVFARLSTFR"
     gene            10525..10896
                     /locus_tag="VIG70_16355"
     CDS             10525..10896
                     /locus_tag="VIG70_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8252171.1"
                     /translation="MNEPRILVADDDKDTVLSLTALLREEGYEVRGVHRGSEVLQAIF
                     NFAPDVVLLDIGMPQMSGYDVARTLRERYGSARPALIAVTGRAGDADRRHAHAAGFEH
                     HVAKPYEPRALLRLIGELSAR"
     gene            10968..12491
                     /locus_tag="VIG70_16360"
     CDS             10968..12491
                     /locus_tag="VIG70_16360"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEY8252172.1"
                     /translation="MKTAVAPRPALRELARFEFENSFVRELPADPVRDNVPRQVANAS
                     YTRVEPTPVAAPRLLGWSRRLGEYLGMAPSDIDVLAGNRVLSGMKSYAARYGGHQFGS
                     WAGQLGDGRAITLGEIVATDETRQELQLKGAGRTPYSRTADGRAVLRSSLREFVCSEA
                     MHFLGVPTTRALSLVGTGEQVVRDMFYDGHPEAEPGAVVCRVAPSFVRFGNFQIHAAN
                     DETDTLRRLADYVIREFFPGQSYVSWYQEICRRTALLLVDWMRLGFVHGVMNTDNMSI
                     LGVTIDYGPYGWLEGYDPQWTPNTTDAQTRRYCYGNQPHIAQWNLTRLAEALLPLAKD
                     KTPLEEGLALYAETFNGAWQRALAHKLGFEALGQPGDDELVSDLFALLYEVETDFTIF
                     FRNLAAVEMEALLPAFYSKAPEGMAGWLQRYAARAKHEPGRVERMNRANPKYIFRNYL
                     AQVAIDALAAGDASVMERLMQVLERPYDEQPEHQDLAARRPEWARDRAGCSALSCSS"
     gene            complement(12493..12999)
                     /locus_tag="VIG70_16365"
     CDS             complement(12493..12999)
                     /locus_tag="VIG70_16365"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEY8252173.1"
                     /translation="MKPLAVLSFAALSIAMSPAGAQSGNHLEVIHHPNYDKSLFMPFS
                     PALKIKSGKLLWLAGGTALPVYHDHPHKREQIMQFMPNDLEAQTRRTMDGIMETLKAA
                     GASPKDVVHVFIFRTRPRMGDIGRSSAVVNSYFAPHNHKPTTTNMAVLELGEPEQLIE
                     IQVVAVVD"
     gene            complement(13041..13622)
                     /gene="alkB"
                     /locus_tag="VIG70_16370"
     CDS             complement(13041..13622)
                     /gene="alkB"
                     /locus_tag="VIG70_16370"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HEY8252174.1"
                     /translation="MKLLRGFALPEEQTFLEAVAEISARAPFRHLVTPGGYRMSVAMT
                     NCGPLGWVSDRAGYRYDANDPESGRRWPPMPDSFLGLAREAAAHAGFRGFVPDACLIN
                     RYEPGARLSLHRDEGDFAVPIVSVSFGVAAVFLFGGLKRTDKAERVLLEHGDVVVLGG
                     EARMRYHGVLPLKPGFHPLTGEQRINLTFRKAG"
     gene            complement(13619..14302)
                     /locus_tag="VIG70_16375"
     CDS             complement(13619..14302)
                     /locus_tag="VIG70_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY8252175.1"
                     /translation="MNDWARIGVELDAQGCTVLENLISPGECDELAAAYVNDALFRSR
                     VVMARHGFGRGEYKYFSYPLPDVIARLRTALYPHLAAIANRWNAAMGIDVRFPEQHAK
                     FIKRCHAAGQRRPTPLLLQYGAGDYNCLHQDLYGEHVFPLQLTILLSEPGRDFTGGEF
                     VLAEQRPRMQSRAEVVTLDKGDAVVFAVRHRPVQGTRGTYRVNLRHGVSRVRSGHRHT
                     AGIIFHDAA"
     gene            complement(14312..14517)
                     /locus_tag="VIG70_16380"
                     /pseudo
     CDS             complement(14312..14517)
                     /locus_tag="VIG70_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003273468.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
     gene            complement(14514..14990)
                     /locus_tag="VIG70_16385"
     CDS             complement(14514..14990)
                     /locus_tag="VIG70_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY8252176.1"
                     /translation="MKELLVYAERIAASLKARKETVAVAESSAGGLITAALLAVPGAS
                     AYCLGGIVIYTRQGWEALRDFDESLLGGHRSATEQNALVRARLARDRFGATWGLGETG
                     AAGPTGNRYGDPAGHACLAVCGRKEISITLKTGSKDRLANMHAFAAAALRLLEESL"
     gene            15065..15817
                     /locus_tag="VIG70_16390"
     CDS             15065..15817
                     /locus_tag="VIG70_16390"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8252177.1"
                     /translation="MRTNTTLVINPKGGSGKTTIATTLASYFAANGTATAVMDYDPQG
                     STMNWLRVRPAHARRIHGANAAPQKTGLRTLGMHVPPGTEQLVIDAPAGASGLVLQDM
                     LSRAHCILIPVAPSPIDIHATANFIRDLLLTGKIRANNIRLGVVANRVRKANPVYEPL
                     ERFLKALNLPLLARIGDSEVFVTAAESGLGIFEMDFAVSAAERGQFQPIAEWVDRVPV
                     AQTRAVPLIAAASRSGGLRVVSSAPSLPGGFR"
     gene            15913..16632
                     /locus_tag="VIG70_16395"
     CDS             15913..16632
                     /locus_tag="VIG70_16395"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY8252178.1"
                     /translation="MRINTTLVVNPKGGSGKTTIAINLASAFAADNVATTLMDYDPQG
                     SSLNWLRSRPPLAPRIHGANGAPGKYGQLRSFDMYVPPETRQLVIDAPAGSAGVLLQQ
                     MVDRAHCILVPVVPSVIDIHASGNFIKDLLSMSRVRMGGARVGVIANKVRVSMPAYQP
                     LLRFLDTLQLKMRARLLDSDAYLKAAEAGLSIFEYEPNQTVAERKQFQPIVDWVTGEY
                     ARPQELAGAVREITHIRRISF"
     gene            complement(16622..17398)
                     /locus_tag="VIG70_16400"
     CDS             complement(16622..17398)
                     /locus_tag="VIG70_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8252179.1"
                     /translation="MRIDGVEVFVEGVGPAIVMIHGWPDTHRLWDAQVEALKERYRCV
                     RFTLPGFELAASKRVHTFEEVLGTIRRVVEQVCPGERITLLLHDWGCFFGYQFAMRHP
                     ELVGRVIGVDIGDAGSRGNRAELGFIGRFMVLAYQVWLALAWRIGGGIGDAMARWMAR
                     VLGCPTYSPAIGSHMGYPYALRWLGAAGGLGRLRAFDPHCPMLFLYGERKPFMFHSRA
                     WAERVAARPGCRVIAFPTGHWIMVQQPREFNAALLAWLRS"
     gene            17487..18635
                     /locus_tag="VIG70_16405"
     CDS             17487..18635
                     /locus_tag="VIG70_16405"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HEY8252180.1"
                     /translation="MNKLLVLDDDPDMCALVVHAAGSAGFEAASATNFEQFKTRLAAD
                     TSVVVLDLMMPEVDGIQVLRYLSEQSYPAGIVLISGYDRKVLKVAAQLAASLGLDIHA
                     SIQKPFKANELREVLAKGGQTRRPLRGGAADSAIDVEELRQAIAGDQLLVYYQPQVHI
                     KTRTLAGLEALVRWQHPTRGLLPATAFIESFETAGLIGELTWLVVRKALADRKKQSSQ
                     AARVPVSVNLSALLLRDLALPEKLLALLAEHGATPADLVLEITESGLMKELHTSLDIL
                     ARMRLKQLHLSIDDFGTGYAMLQQLQRVPARELKLDISFVQAMLADESADIILRKTLE
                     LAHDLDMSVVAEGVETVEQLNRLAEYGCDVAQGYLLGRPSPTGALHEI"
     gene            18700..18891
                     /locus_tag="VIG70_16410"
     CDS             18700..18891
                     /locus_tag="VIG70_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252181.1"
                     /translation="MDNTAKQHLLQRAADLLGRGEIARRLNTPPPVLEAWICGEASMP
                     DGKLLVLAAALEEHAKTGK"
     gene            complement(18911..19624)
                     /locus_tag="VIG70_16415"
     CDS             complement(18911..19624)
                     /locus_tag="VIG70_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEY8252182.1"
                     /translation="MQMIRSDLAWSTVDRIVVRGKDLPKEILGHLNLGDMAFLEITGR
                     VPTANESKIFNAMVVTLVEHGVTPSALAARMTYVGAPEAMQAAVAAGLCGLGSVFVGS
                     MEGCAKLLTESKDKTAEQIVAENKRIPGLGHPLHKPVDPRSVRLFEIARETGHYGIYC
                     KLIEEVSRLKGLTLNATGAIGALACELKLDWRAVRGLGVIARAIGLVGHILEEANQPM
                     ALELWQRTEEEASRHAKPR"
     gene            19718..20320
                     /locus_tag="VIG70_16420"
     CDS             19718..20320
                     /locus_tag="VIG70_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY8252183.1"
                     /translation="MIRGFHLADFFTLANAACGVGAIFLAMLYLKSGAQAHFYAAAAM
                     APLAFFFDWLDGNVARWRHQHSALGRELDSLSDVISFGVAPAVLAYAAGLNGGWDAAA
                     LIYFVCCGVSRLARFNVTAETLAAGSDSGKVAYFEGTPIPTSVVLTAVLAFAAWQGRI
                     GENLYGGAWNLGPWQFHPLSLLFIASGSMMISKTLRIPKP"
     gene            complement(20323..21054)
                     /locus_tag="VIG70_16425"
     CDS             complement(20323..21054)
                     /locus_tag="VIG70_16425"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8252184.1"
                     /translation="MSGGLTLGAALILGIAASGHCLVMCGGISSALGLASARSPHGQP
                     RVPLIISYQIGRTASYAIAGALAGGAVGTFVDWLDMDAVRRGLRALSAVALVVAALVA
                     FGTLRDPGSRAGQLLWRRIAPLGRRLLPVTNVGRALGFGMVWGWMPCGFAYTVILIAA
                     LEQDALRAAATMLAFGMGTAPTMIALAYGARRAASLAAGGTARRVAGTVLICSAALTV
                     LAPQIVAASPWLHPWVQYLCRVDAP"
     gene            complement(21051..21212)
                     /gene="ccoS"
                     /locus_tag="VIG70_16430"
     CDS             complement(21051..21212)
                     /gene="ccoS"
                     /locus_tag="VIG70_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEY8252185.1"
                     /translation="MTSLLFLIPLSVLVLVGAGIALFWAINRGQFDDLETPALLPLLD
                     TDDAPRERE"
     gene            complement(21209..23656)
                     /locus_tag="VIG70_16435"
     CDS             complement(21209..23656)
                     /locus_tag="VIG70_16435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY8252186.1"
                     /translation="MTGTCWHCGESLPPDPPQANVAGIRRAVCCNGCRAVVEWIADLG
                     LADYYRLRTGPAARAPDRRESEASAAAFLRPELSRHLVRPLADGSSEAIVLIDGMHCT
                     ACCWLIERTLGRLPGVVDVSVNAGARRARIVFDAKALPLARIVDALERVGYRALPLDR
                     DALDDTRRRETRDAQKRLAVAGFGAMQAMMYASALWFGAFDGADAATRDFFRWLTLLA
                     ATPVVFYSAAPFFTGAFRLASARRLGMDVPVALAVALIYAGSVIEIFAGGRDIWLESV
                     SMFIFFLCTGRYLEMRARHHAGDLSDALARLTPVFADRVQADGTLLRVGAFELVPGDR
                     VVVGDGGSVPADGMLETEQCRVDEALLCGESTPRHRRRGEALCAGSIVVGTPATMRVT
                     RVGADTVVAGIVALTTRAASSKPRLARAGERAAAGFVARVLVLAICTAIGWAIVDPSH
                     ALAATVAVLVVACPCAFALAAPAAVTRALAVLTGRGVLVVRPDALEDLASVTHVVFDK
                     TGTLTEPAIAPGLAATRQGVDRDAALAVAAALAQGSRHPLARALVAETPATLPAVEAR
                     ESFAGRGIGGVVGGRGYRLGRADYALGCESFPASCPADLEDAVVLADADGLVAAFRVD
                     ERLRPGARAAVDALARAGIDVTIASGDARSRVEAVAQRLGVAAWKARQSPTDKLALLA
                     SLRARGARVAVVGDGVNDAPVLAGADVAIAVGTAADAAQAASDIVITGQLDALAAARA
                     VARQTLSILGQNRRWALCYNLATVPLAALGFVPPWLAAIGMSASSLAVVVNALRIGPV
                     EETPVAGEAIVRRGALA"
     gene            complement(23735..24634)
                     /gene="ccoP"
                     /locus_tag="VIG70_16440"
     CDS             complement(23735..24634)
                     /gene="ccoP"
                     /locus_tag="VIG70_16440"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEY8252187.1"
                     /translation="MTELWSNWVIVLVSINLGITVFLFLWGLRVDIPTLPDGTSGHVW
                     AHGVLREGVRRLPTWWIVLSAASLIAGLGYLVLYPGVGAFAGTLGWTSGSELAQKQES
                     NRLLEAPLLERVRGKPVETIAADPEALRVGGVLFIENCAACHGRDARGNRALGVPDLT
                     DRDWLYGGDGKAILTSILDGRNGTMPPLGSGKSEREIRDIAHYVLKLAGRTHDSLRAH
                     FGKEGFAACAACHGADGKGNTALGAPNLTDGVWLYGDSIEDIVESIRNGRNGVMPALR
                     HRLGEDNASLVAAWVYAQSHPTQ"
     gene            complement(24631..24786)
                     /locus_tag="VIG70_16445"
     CDS             complement(24631..24786)
                     /locus_tag="VIG70_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEY8252188.1"
                     /translation="MNPLWGQLIGFVTLVLMLVFIGIWAWAWLPFHKREFDALAKLPM
                     EDAEDER"
     gene            complement(24783..25394)
                     /gene="ccoO"
                     /locus_tag="VIG70_16450"
     CDS             complement(24783..25394)
                     /gene="ccoO"
                     /locus_tag="VIG70_16450"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEY8252189.1"
                     /translation="MARGYKYFEAIEKNAGLLGILIVVMVSLGGLAELTPLFIQAHAV
                     APAPGVKPYDPLRLAGKDVYVREGCYLCHSQMIRALRAETQRYGAHSTVAESVYDRPF
                     QWGSKRTGPDLARVGGKYSDAWQRLHLVDPRAVVPESNMPAYRWLASAQLDGNDIAAR
                     MRTLRALGDPYSESDIDAAPKAVEGKTELDALVAYLQGLGASR"
     gene            complement(25407..26873)
                     /gene="ccoN"
                     /locus_tag="VIG70_16455"
     CDS             complement(25407..26873)
                     /gene="ccoN"
                     /locus_tag="VIG70_16455"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEY8252190.1"
                     /translation="MRTAETYNDEIVRLFLLAAAVWGIVGMAIGVYVAAEMVWPGLDF
                     GVPWLSFGRLRPNHTFGVIFAFGGSALMGTCYYVVQRTGHTRLALGGLANFTFWGWQL
                     GCVLAMATMPFGITQSKEYAEPEWFIDIAVAIVWVSFGIVFFATIARRRIRHIYVANW
                     YYAAFIIAVGLLHVVNNIALPVSLTKSYPVYAGSVDAMVQWWYGHNAVAFFLTAGFLG
                     MMYYFVPRQAQQPLWSYRFSIVNFWALISIYMWAGPHHLLYTALPDWVQSLGMAFSLI
                     LLMPSWGSAANGLMTFNGSWHRLKTDPAAKFMVVALVFYAAATLEGSMMAIRTVNALS
                     HYTEWTIAHVHSGALGWVAMITIGSLYAMAPRALGQPAMHSHGAMELHFWLHVVGVLL
                     YVFSMWIAGITQGLMWRATEADGSLTYPFIDSLVAIKGLYGVRWFSGLLILSGMVVMA
                     WNLWHTAAAARAHLIKPIPVPIPEPEPAQVPAPLPATG"
     gene            complement(27087..27380)
                     /locus_tag="VIG70_16460"
     CDS             complement(27087..27380)
                     /locus_tag="VIG70_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY8252191.1"
                     /translation="MPKAYWVICHRSTKSPEKFAAFTKLAYPAIQAAGGRFLVRTSKV
                     AKVYESGIEERTVVIEFENLAAAVAAYDTADYKEALRALAGGAERDLRIVEGV"
     gene            complement(27410..28147)
                     /locus_tag="VIG70_16465"
     CDS             complement(27410..28147)
                     /locus_tag="VIG70_16465"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEY8252192.1"
                     /translation="MTLTRIDCGTGAKPTNVNERFSDTFAYKDLNLVFTFSCYLIKHG
                     DEYMVWDTGFAPGSNPNAPKVGIVDRLKELKVTPDQVKYVGISHFHGDHTGQLINFPK
                     ATLLIGKGDWEGVSANPPMQGANALGFTPWTKDGGKVEPLAIDKDVFGDGTVVVLRTP
                     GHTPGHSCLLVRLKEKGPVILIGDLAHFRENYESNGVPWFNADRAQTLASLERVKKLA
                     QNLNATVVIQHDMRDIGKLPAFPAAAK"
     gene            28383..29135
                     /locus_tag="VIG70_16470"
     CDS             28383..29135
                     /locus_tag="VIG70_16470"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8252193.1"
                     /translation="MSIRRHVSSAALVTLFAAAGCVSQGEFDKLQSAKNQEIASLQSS
                     RSTLEGQVRNLENERASLEKQKDSLTKEKDSLTQQVAELEAQRARLQASEKQVEARYD
                     RLRADLSEEVKKGQLQIRELEGKLTVDVAEQLFFDSGRAALKDTGKQVLQKVADSLKS
                     YEDKAIRIVGHTDNVPISGGLQKVFPSNWELSAARATTVVRFLQDNGIPPERLVATGR
                     AEYAPVAPNDSAEGRQKNRRIEITLIDKGLVQ"
     gene            29292..30842
                     /locus_tag="VIG70_16475"
     CDS             29292..30842
                     /locus_tag="VIG70_16475"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY8252194.1"
                     /translation="MMAQQNPLERYEILNELGRGAIGRVYAARDRSTGTVVALKTLDP
                     ALFNEPDATVAELFLKDAVSAGRLRHRNIVKVHDAGEAGGTACVAMELLEGESLRSIL
                     DAGPLSIARAIQIFDDIASALAYAHEEGVVHRGVKPSNIIVLRSGVAKLGDFGVGRLG
                     EAALLSGQRGDCIRYMSPEQVRGDPVDHRSDLFSLGAVFYEMLTRQALFEGDSAKEIT
                     QNILHAEPPLPSKVNPLVPFALDRIVFSMLADHPDDRFANARVLLRELQGLEEGLGLG
                     PGAGAGAGGPTASVPPMQDAPRFAVDEHDARFRMDRGPEPERSSGSRAARFAALALLI
                     AVLPIGLGVAWYYSPGPSEPAPSPSTAPPPVAEATKEPATAPAAPEESPPRAMVDARA
                     EQEPSGIASAPNPLPPKPLVAEPSPPAQEVPAAAQPTAKISEPQPGGTAKLIIAVSPQ
                     GELYIDGKHYGTTPPLTTLDLEPGMHRIEIRSGSRKPYLTYMTVQAGDQRRIRYDFAA
                     KGSRPPSR"
     gene            complement(30863..31816)
                     /locus_tag="VIG70_16480"
     CDS             complement(30863..31816)
                     /locus_tag="VIG70_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY8252195.1"
                     /translation="MKTVYALVLALLTAGGALAQQDRPVRFVLPNAAGSGVDAITRAA
                     QPAFAKAFGAAVVVENQTGAGGVIGLQTLARSAPDGHTLAVVSNNVVIFPSVLKSLPF
                     EMPGDFTPIARVGSTPMVLVVNPSKVQATNHKELVALVKGKPDALNYASSGSGTILHL
                     AVELYLEAAGIKVRHIPYKGVGPMVADLLGGQVDFAAAALPSVQGHIASGKLRAIGMF
                     PAQRTAAAPDIPTFAEQGLANFEVEAWFAVIGPKGMSPATVKKVHDAIVAAFNDAAVK
                     EAMAKQGNTIRVTTTEQAQAAFHTELAKYAALVKKVGLEPQ"
     gene            31858..32262
                     /locus_tag="VIG70_16485"
     CDS             31858..32262
                     /locus_tag="VIG70_16485"
                     /inference="COORDINATES: protein motif:HMM:NF018813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEY8252196.1"
                     /translation="MALRSTIYRADLSVSDLDRQVYGRHALTLARHPSETEERLMVRL
                     LAFALHSDEALEFGCGLSAEGEPDLIRRDPTGAVDLWVEVGLPDERDVRKACGRSREV
                     AILAYGARRVESWWSATMPRPSCGNRTCGCSR"
     gene            32250..32423
                     /locus_tag="VIG70_16490"
     CDS             32250..32423
                     /locus_tag="VIG70_16490"
                     /inference="COORDINATES: protein motif:HMM:NF018813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252197.1"
                     /translation="MLTLTGEETAALQTLAARTMSLTSTVQEQHVWLASATSTIELAP
                     VCRTPDRNEKGAA"
     gene            complement(32455..>32885)
                     /locus_tag="VIG70_16495"
     CDS             complement(32455..>32885)
                     /locus_tag="VIG70_16495"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY8252198.1"
                     /translation="YRELAILRTGFAGGSRFVFSQHSKFARAAGLSEEKVAAVPAWTS
                     AEAFDAKERAVLGYVDELVLADGRVQDATFERLKRHFDDEQILELTYVTCLYKAWATF
                     SRGLRLEFDDIDERVTEVPAPAGGAAIDMKTAMLGDDKVK"
BASE COUNT         5625 a        11236 c        10856 g         5168 t
ORIGIN      
        1 atcgtcgatc aggacctcgc acaccagctt ctgcgcgccg taggaaaggc gcggcgccac
       61 gggggtttcg tcagtcactt catcgacgcc cccgtaaacg gcgatcgacg aggcgaacac
      121 gaatttcggg cagtgcccgg cgaggcggca ggcttcgagc aggtcgcgcg tcgcgtcgag
      181 gttcaccttg gtgccgagct cgaaattctg ctcggcgccc cccgagacca gcgacgccag
      241 atggaatacc gagccgatgt ccggtgtgat cagcgaacgg gcaaactgcg ggtaggcgat
      301 atttccgatg accggcgccg gggacgactg gtcggtcgcg aggacgtcgc gccccgccag
      361 cgccttcacc agcgcggaac cgatgtagcc tgccgcgccg gtcacgagta ttctcatggc
      421 aacaagcatt ctaaagagaa ggaggagagc gtggtccgca ccctgttggc gctgttgctt
      481 ctttcgttcg gctctgccgc gtggacgcag agctatccct cgaagccggt taggatcatc
      541 gtacctttcg gtgtcggcgg ctcggcggac gtttatgcgc gctacctcgg cgccaagctg
      601 caggacagcg tcggccagag cttcgtggtc gagaaccggc ccggcggcgg ctcgatcgtc
      661 ggcagcgacg tggtggcgaa gtccgatccg gacggctaca ccctgctgat gatgtcgaac
      721 acccatacgg tcaacgaaac gctcattcct aaaaagccgt tcgagctgat gcgcgatttc
      781 gcgccgatca ccggggtgag ctattccgac ctgatcatgg tggtgcatcc ctcggtgcag
      841 gcggcgagcc tcaaggaatt catcgcgctg gcgaaatcga agcccggcgc gctgaactac
      901 gcatcctcgg gcaacggcac gccctaccac atggcgggcg agctcttcaa atcgatggcg
      961 ggcgtcgaca tcgtccacgt gccgcacaag ggcagcgacc aggcgcgcgt cgcggtcatg
     1021 ggcgggcagg tgcagatgat gttcgacgcc gtgccgacca tggcggcgaa cgccaaggcg
     1081 ggcaaggtca aggcgttcgg caccacgggc gagaagcgct cgagcgtgac gcccgagctg
     1141 cccacgctct ccgaggcggg cgttgcaggc tacgagaccg gcctgtggct tgggctgatg
     1201 gcgccggcag gaacgccgcg gcccatcctc gagcgcttga attctgaagt aaacaagctg
     1261 ctcaactcgg ccgaggtcaa gcaaaactgg gccaagcagg gcaccatcac catgggcatg
     1321 tcgatcgacc agttcgacaa gttcctgcgc caggacatcc agaagtggtc caagctggtg
     1381 aagtccacgg gaatgaaagt cgattgacga acgagcagct cgcgatcgtc gaggtcgtcc
     1441 acaactgggc gctatggcgc gacgccggcg actggcagcg cttcgcgagc gtctggcacg
     1501 acgacggctg gatgaccgcg acctggttcc aggggccggc gcaggagttc atcaaggtga
     1561 gcaaggccgg cttcgagaag ggggtgagca tcctgcactt cctcggcggc tcgagcgtgg
     1621 acgtgaaccg cactcgcgcc atcgcccaga ccaagatgac catcagccag cgcggcccgt
     1681 gcgagggcga gctggtcgac gtcgtctgca ccggccgttt ttacgatttc ttcgagaagc
     1741 gcgccggccg ctgggcgatc gtgcggcgcc agccgatcta cgagaaagac cggatggacc
     1801 cggtcgaccc tgccgttcgg atcaagctgg accctgattt gctcggttcg atccccgagg
     1861 gctaccgcca tctgggctat ctgcagtcga agctcggctt ccagatcaag cgcgatttgc
     1921 cgcagctgaa gggcgcggcg gtcgagctcc tctaccgcca gggccgcgcc tggctcgacg
     1981 gcgccgcgtc ggctttcccc gacgaggaga gaccttagtc ctagcgggct tgctgcgcgt
     2041 tggcggggcg caatacgcgg gcttcagcat gcgtgggtga cccccgctgc acagcctttc
     2101 gaggtagtgg tcgcccggct ggacgctccc tccgacgagc tgcgcgctct ccgggaatcg
     2161 ctttgcgcgg ccgagcagca tcgcgccggg cgctttcgct tcgagcgcca ccgccgccgc
     2221 ttcgtcgtcg cgcgcggccg cctgcgcaag ctgcttggcg agcgcctcgg cgtgccggcc
     2281 gaaaaggtcg agctcgcgca cggcgcgaac ggcaagccct gcctcaagca caacggctgg
     2341 cacttcaacg tctcgcattg cgacgacgtc gcgctgttcg cattctcgaa agcgttcgag
     2401 atcggggtcg atgtcgaagc gatccgcccg atccgcggcg ccgacacgat cgcggcgcaa
     2461 ttcttctccc cgcgcgagaa ggcggcgtac gccgcgctcg cgccggatga caaggcgctc
     2521 ggcttcctgc actgctggac gcgcaaggaa gccctggtca aggcgatcgg cgagggcctc
     2581 tcgatgccgc tcgagcggtt cgacgtggcg cacgcgccgg gctggcgcct gcacagcttt
     2641 ttcccgcttc ccggcttcat cggcgcagtc gcctgccaac atggatagat tgcaaattcc
     2701 gcttaccgct tacgagtctc cctgggtgaa cgacgacgtg cggatgttcc gcaagacggt
     2761 cagccagttc atcgagaagg aattcgttcc gcaccagccg cggtggcgcg agaaccgcgg
     2821 ccccgatccc gagtcgtggg ccaatgccgg ccgcgccgga atcctgctcg ccgacctgcc
     2881 ggaagaatac ggcggcggcg gcggcacgtt cgcgcacgag gcggtggtgc tggaggagct
     2941 cgcccgggcc ggcgtgcact tcggctccgc catccagagc acggtggcgc attacatcct
     3001 cggctacggc agcgaggagc agaaacgcaa gtggatcccg cgccttgcgc gcggcgaaag
     3061 cgtcgccgcg atcgcgatga gcgagcccgg cgcgggctcc gacctgcagg ccatcaagac
     3121 cagcgcccgg cgcgacggcg agcactacgt gatcaatggc tcgaagacct tcatcaccaa
     3181 cggcgcgcgc gccgacctgg tgtgcctcgc ggcgaagacc aacccgaagg ccatcgggat
     3241 gcgcgcgatc tcgctcatca tggtcgaaac gaaagacctg ccggggtacc gcgtcggccg
     3301 cgcgctggaa aaggtgggca tgcacggcca ggacacggtc gagcttttct tcgacgacgt
     3361 gcgcgtgccg gtttcgaacc tgatcggagg cgcgagtgtg gccaatgagg gccgcggctt
     3421 ctcccagatg atggagcagc ttccctacga gcggctgcag atcgcggtgg gcgccgtcgc
     3481 gatggccgag caggcggtcg cgatcaccgc gcgctacgtc aaggagcgca aggcgttcgg
     3541 aagaccgctc ctcgacctgc agaacacgcg cttcaagctg gccgaatgca agaccaacgc
     3601 ccaggtcggc cgcgtgttcc tcgacaactg catcgagcgc ttcatcgccg ggcgcctcga
     3661 cgagccgagc accgcgatgg ccaagtactg gctcaccgag agccagtgcc gcatcgtcga
     3721 cgagtgcctg cagctccacg gcggctacgg ctacatgacc gaatatccca tcgcgcgcat
     3781 gtgggccgac gcccgcgtgc agcgcatcta cgccggatcg aacgagatca tgaaggaact
     3841 gatcgcatgg tcgctttgaa gctttgcctg cacgagctga tcgaagcgca ggcggcgcgc
     3901 acccccaacg cccccgcgct ggcattcgag ggccaggcgc tcacctatcg cgagctcgac
     3961 ctgcgcgcgc gccgcctggc ggcgcacctc gcaggcctgg gcgtaggtcc ggatgtcacg
     4021 gtgggtctct tcgtcgagcg ctcgctcgag atgatcgtcg gcattctcgg catcctgaaa
     4081 gcgggcggcg cctacgtgcc gatcgacccc gcgtatccgg cggagcgagt ggcctacatg
     4141 ctctcggatt cgggcgccgc cgcagtgctg acgcagtcga gcctggcgga caaggtgtcc
     4201 ggcgtgcagg cggtttgcct ggatgccttt gcatggaaca aggacgtgcc ccgaatcgaa
     4261 gctcccgtgc agtcccgcca cctcgcgtac gtgatctaca cgagcgggtc gaccggccag
     4321 ccgaagggcg tgggcatcga gcatcgcaat atcgtcaact atgtgctcgg cgtgatcgag
     4381 cgcctgcagc tcgagccggg aatgcgctat gccgtcgtgt cgaccattgc cgccgacctg
     4441 ggcaatacgg tgatctttcc ttcgctcgcc accggcggct gcctgcacgt catctcgcag
     4501 gagcgcgccg agaacggcgg cctcctcgcc gagtacttcg agcgcgagaa gatcgacgtg
     4561 ctgaaggtcg tgccatcgca cttcgcggca ttgaacccgg aaggcgtcat gccgaaaagg
     4621 cgcctcatcc tgggcggcga ggcatcgcgc ctcgactgga tcgagcggct gcgcgccctc
     4681 gcgcccgcct gcgagatctt caaccactac gggcccaccg agacgacggt cggcgtgctg
     4741 acctgccatg cggccggccg gattcccgcc acgcagtcgg gctccctgcc cctgggcaag
     4801 ccgctgccga acgtcgaaat ccgcctggtg gatggcgaga tctgcatcgg cggcgccggg
     4861 gtggcgcgcg gctacctcaa ccggccagag ctgaccgcgg agaagttcgt cgacggcttc
     4921 taccgcaccg gcgaccgcgg ccgcgagctt gcggacggct cgatcgagtt ctgcgggcgc
     4981 atcgatcacc aggtcaaagt gcacggctac cgcgtcgagc tcggagaaat cgaggcggca
     5041 ttgcgctcgc atgagaaagt gcgcgacgcc gtcgtgctgc tgcgcgaaga ccagctcgtc
     5101 gcgttcgtcg cgccgagcga ggcggacgcg cggacgctgc gcgagcacct caaggcgcgg
     5161 ttgccgcagt acatggtgcc gtcggtcgtg gcgccgatgg agcggctgcc gctgacgccg
     5221 aacggcaagg tcgatcggca ggcgctcgct cgcttcacgg tccagaaaga aacccctgcc
     5281 gccgcgccgc gcaccgagac cgagaaggcg ctcgccgaaa tctggggcga gctgctgcag
     5341 gccgaggtcg gcgtcgacga ggacgtgttc gacctcggcg cgcattcgct aatggcgatg
     5401 aaggcgctca cccagatccg cgaccggttc caggtcaacc tttcgctgcg caatcttttc
     5461 gagcacccga cggtcgccgg gctcgccggc gtgctcgatg gcgtctggct cacccaggcg
     5521 agatcccccg cgagcggcgc gcaagaggag ttcgttctgt gaacgccgcc accttcctgt
     5581 ccgagctgcg ccgccgcgag atccaggtcc gggcggtcgg cgaccagctc catttcaccg
     5641 ccaaggctgg cgcactcact cccgagctgc gcgagcagtt gcgcgcgtgg aagaacgaca
     5701 tcctgaaact gctggcttcc gcgcaggcgc tcgatacgca ggagcgcgcc atcgtgccgc
     5761 tgcaggcgaa aggaacgcgg gtcccggtct tcgcggtacc cggacataat ggggatgtct
     5821 tctgctatcg cgctctggcg cagcgcctgg gcgaggacca gcccttttac ggcctgcagc
     5881 ctcccggggt cgacggcaag gacgagccgc tcgggcgcgt cgaagacctc gcggccagct
     5941 tcgcgcggca gatccgcgtc ttccagccga aggggccgta cgtcatcgcg ggctactgcg
     6001 ccggcggcac cgtggcgttc gagctggcgc agcagctttc gcgcgacggc gcggcggtga
     6061 acctgctcgc cctcttcggc agcccctatc cggcgtactt caggcttccc gcgcagctct
     6121 tgcagcgcgt ggccgagcac gcggcgcgca tcggcggcct tgcgcgcgat ctcgccgtgc
     6181 gctcgccggc gcagtatcgc cgctatttcg cggaaaggat gcgccgccac cgggatcgcg
     6241 tcagcgccgc ggcggctgcg gccgcggacc cggtactcgc gctgcgcgcg agggtggagc
     6301 gcgctacggt cgcggcggtc cgcgcctaca cccccggcga cttcgacggc cgcgtggcgc
     6361 tcttcctgcc ctcgatggag tgggacctcg cgcagcgctg gcgctcggtc gcgccgcacg
     6421 ccgagcaata tttcggcccc gatgcctgca gcagccagga catgctgcgc gagccgaatg
     6481 cgacaatatt tgccgagctc ttcgcggccg cggttcaacg ttggaaaatg ccctaatatg
     6541 gttcgtcgcc ttccttggcc tggcgattgc cgggctgggc gggctgttcc tctacggctg
     6601 gcggcggcgg aaagagaagc cgccgccggg cgtgaaaccg ctcagggacg aagacgacga
     6661 ctgggggcga tgacatgacc aagaaacagg gcaggcgcgc cgacgacatc agcgccgagg
     6721 aaatcaagca attctggcac ggcttctgcg agcgcaaggg tgtcgacgcc gcgactcgcg
     6781 cgcgcggcga tgccaagatc gaccaggatc cggaacactg ggccgaccag acgatgctca
     6841 agctgctgga cgctgtcgcg atctagcaac tccgcatttg cactacccga gattcgggca
     6901 gcgggtgact tgagcaggcc gggtcgccgc cccaccatcg ctcgatggag aaaatcgaaa
     6961 ttcccgcaga caactgcccc gtggcgccgc cgcggcgcat cacggtccat acgcgcgtgc
     7021 tgcaccgggc ctgtcagcac ctcggcgggg tcgagcatct cgcccggcac ctcaaggtat
     7081 cgccggacct cctgcactcg tggctcgagg gtgaagacgt gccgccgtcg agcatcttcc
     7141 tcaaagcagt ggatctcgtc ctgccggcct ggggacccga cgacgacgcg ctggccaaga
     7201 ccatctccgc ctcgcgcccg aaaaagaact agcgaatcaa gaagtcgtcc ccgcgaaggc
     7261 ggggacccag ttttcttttt tgaagaacat aggttcccgc cgcgggaacg acggttcttt
     7321 cagagtttcc ctagtggtag agcagcaccg cctcgcgctc ctcgcgccag gcgtcctcgt
     7381 ccgcgcgcgc ggccgcgtcg agatcgcccg gcttcgcgcc cgcgtcgtcc acccactgcc
     7441 gcagcaggct gccgccgttg atcacgtcga tcgcgagccg cgtctcgtac tcatagggaa
     7501 aatcgcgcca caacgggtac tccggcgcga ggctgcggat cgccttgagc gcgagggcga
     7561 cgatccgcca cggccggaag gccgcgtggc cgtaatgcgg cgcctccgca tggatctgca
     7621 acccggcgca cagcttccct gcgtgcttgt ggaaggtcgg ctcgaaccag cagggccgca
     7681 gccggcagcc tcgcagccag cggggttcga gggaatgcat ttttttgaga atggctgccg
     7741 agtccagatc tggagagcca agaacctcca gcggccgggt agtgccgcgt ccctccgaga
     7801 gcgtggtgcc ttcgagcatc acggtgccgg gatagcagcg cgccatgccg agattcggcg
     7861 cgttcgggct ggggttgacc caggcgcgct cgccgagcgg ccagccttca gcgggcttcc
     7921 agcctttcat ctcgacgacc tcgcagtcca cgtcgatctt cgcggaccgg acgaaccact
     7981 tcgcggcctc gccgagggtg agcccgtggc gcatgggcag cgctccggcg ccgacgaagc
     8041 tttcccagcc ggggcgcagc ttcaggccct cgacgaggcg gccgatcggg ttcgggcgat
     8101 cgagcaccca cacactcttg ccgtgctgcg ccgccgcctc gagcacgtag cgcagcgtgg
     8161 tcaggaacgt atacacgcga cagccgaggt cctgcaggtc gacgagcagg acgtcgaagc
     8221 gctccatcat ctgtgcgctc gggcggcgca ccgcgccgta caggctgaac acgggaatgc
     8281 cgtgcaccgg gtcggtgaag tcggaagact cgaccatgtt gtcctgcttg tcgccgcgca
     8341 atccgtgctg cggcccgagc gccgcggtga gcttcacgcc ggcggcggcg agcgcgtcga
     8401 gcgcatgctc gaggtcctga gtcaccgacg ccgggtgcgc gagcagcgcc acgcggcgcc
     8461 cggcgagccg cttcgccagc gcgcgctcgg cgagcagccg gtcaatgccg aacttcatcc
     8521 gagcgccaat gcgaatgcct gcgcatgatg gaaatccggc ttgccgggag cgtggcgcag
     8581 cgcccaatac gaaagcgccc cgctttcctc ctcgatcacg gccgagagac cgatcagcat
     8641 tttttgggtt tcagggaaag ggattgacgc ggccagctcg agccggccgg cgccctgccg
     8701 gaccacgatt tcagaaggaa catcgaggga ccggccacct tcgcggtagc cctcgaacgc
     8761 ataggccgcc cactcgcccg acggcgaaaa gttgaactcg cgatagcccg gctcgcccgc
     8821 gcgagcgacg aagagctcgc agcaggtgtg ctgccacaag ccctccgcaa cgcgcggcgg
     8881 ccgcggcggc gggatgcgca gccggtcgat ctcgccgtcg agcacgtagg cgacctgcat
     8941 gcgccgaggt tttcgcgtca gggaaacctt gatgccgcgc aacgcggcgc atgaagtcgc
     9001 gggatggcgc tcgagcgcgg cggacggcat ggccgcgcga gcctatcatg ggcatcgtga
     9061 aagggctggg gacgctggca gtagcggcac tcgtgggctg ggcgtttctc gctccgccgt
     9121 cgggctcgtg ggcctttgcc gcggcggaat tcgcgtttct cgcctggctt gcgcgcgagg
     9181 tgcgcaaggc cgacgcagcg gcgctgcgcg ctcgcgcgct ggagcccctc gagcccgacg
     9241 aagccgacat cgtgcagcgc tacccgcttt acttcgcgcg gccggcgctc gcgcgcgaat
     9301 gcgcctcgac gctctcggcg ctcggcctcg cgagcctggt ggtggtcccc tggctgacct
     9361 acaaagtgca gtgggcgccg gcgatcctga tcggcgtatt cctgttcgcg gtggcgcggc
     9421 tcaccaagct gcttagcccg gtgtacgcgc tgcggctggc gaccgccaaa ggcgaccgca
     9481 aggcgctgcg cctgctctcc gcgcacgacg gcgcggcgcg caagctgtca gcccatcatg
     9541 ttccacagcc agaagagcac cgcgccgccg gccggtagga agatcgcgaa cctcagcacc
     9601 gacatcacgc ggttgcggtc ctggggttcc cattccggct tgaggtgccg cgctgcggcg
     9661 cgctctacga cctgcttgtt gaccgcgtaa tactcgtagc cgacgagaat cgccccgaaa
     9721 acgaggccga ccagtgcgcc ttccagccaa ggggccatgg cgcgcgactc tagcggaatg
     9781 tcgataaccg ggcaaacacg cgcttcatcg cgttgacgcg ggcaaggtag gcgcgcgggt
     9841 cgtgctcgcc gcagcgctgg cctggagcga ggcggaagcc gcgccgggca taggagtcgc
     9901 ccgccgccgc gccgcacagg tggatgatcg ccgcgaggtc ctgcttctgc tccagggtcg
     9961 ccttgaggcg gtggcgctcg agcaccatgg ccacgcggcg gtcgaggtag gccgaggtga
    10021 gctcgaccgc atggctcggg atcacccgca tgtagagcga attgaaccag cagccgtcct
    10081 cgagcaccgc gttgtcgcgg atgcagtagc gcttcgcctc gcgaaaagtg ccgtcggtga
    10141 tctggtacat gcccaccgcg ctcgaagccg gccggtaaac ctcccgcgga tcggagctcg
    10201 cgcgcaggcg ccagggcgtc cgggcgaccg gattacccga gccctcgacc tgcgcgagcg
    10261 cggcgagcag ctcgggcgtc atgactgcgg tcgagtgcct gcggaagatc ggttcgtagt
    10321 gctgccaggt ctccgtcggc gtcttgaaca gcgcgccgct caccggaaag aaaagctcgg
    10381 ccggctttcg gacgacctga taaacccagt tgagcgccag cgcagccgcg accaagccga
    10441 gcacgccgaa aatgatgtgc ttgcgcgacc tcatgtagct gcgcgacgac agacggcgca
    10501 cgcccctctt cgtagaatcc tcctgtgaac gagccgcgga ttctggttgc cgacgatgac
    10561 aaggatacgg tgctcagcct gaccgcgctg ctccgcgagg aaggctacga agtgcgcggc
    10621 gtgcaccgcg gctccgaggt gctccaggcg atattcaact tcgcccccga cgtggtgctg
    10681 ctcgatatcg gcatgccgca gatgagcggc tacgacgtcg cgcgcaccct gcgcgagcgc
    10741 tacggcagcg ctcgcccggc gctcatcgcc gtgaccggcc gggcgggcga cgccgaccgg
    10801 cggcacgcgc acgccgcggg cttcgagcac cacgtggcga agccgtacga gccgcgcgcc
    10861 ctgctgcgcc tgatcggcga gctctccgcg cgataaggga attcggggac ggagcccgaa
    10921 ctaattaggg ctccgtcccc gaattcttgc tgcacaatcc acgttgaatg aaaactgcgg
    10981 tagcgccacg ccctgccctg cgcgagctcg ccaggttcga gttcgagaac agcttcgtgc
    11041 gcgagctgcc ggccgatccg gtgcgcgaca acgtcccgcg gcaggtcgcc aatgcctcct
    11101 acacgcgcgt ggagccgacg ccggtggcgg cgccgcggct cctcggctgg tcgcgccggc
    11161 tcggggaata tctcggaatg gcaccgtctg acatcgacgt gctggccggc aatcgcgtgc
    11221 tctccggcat gaagtcctac gccgcgcgct acggcgggca tcagttcggg agctgggccg
    11281 gccagctcgg cgacggccgc gccatcaccc tgggcgagat cgtcgccacc gacgaaacgc
    11341 ggcaggagct gcagctcaag ggcgccggcc gcacgcccta ctcccgcacc gccgacggcc
    11401 gcgcagtgct gcgctcctcg ctgcgcgagt tcgtgtgcag cgaggccatg cacttcctcg
    11461 gcgtgcccac gacgcgcgcg ttgagcctcg tgggcaccgg cgagcaggtc gtccgcgaca
    11521 tgttctacga cgggcatccc gaggccgagc ccggcgccgt cgtctgccgc gtcgcccctt
    11581 ccttcgtgcg cttcggcaat tttcagatcc atgcggccaa cgacgaaacc gacacgctgc
    11641 ggcgcctcgc cgactatgtg atccgggagt tctttccagg acagtcctat gtctcctggt
    11701 accaggaaat ctgccggcgt actgcgctgc tgctggtcga ctggatgcgt ctgggcttcg
    11761 tgcacggggt catgaacacc gacaacatgt cgatcctcgg cgtgacgatc gattatgggc
    11821 cgtacggctg gctcgagggc tacgacccgc agtggacccc gaacaccacc gacgcgcaga
    11881 cgcgccggta ctgctacggc aaccagccgc atatcgcgca gtggaacctc acgcggctgg
    11941 cggaagcttt gcttccgttg gcgaaagaca agacgccgct ggaggaaggc ctggcgctct
    12001 acgccgagac cttcaacggc gcctggcagc gcgcgctcgc gcacaagctc ggcttcgagg
    12061 cgctcggcca gccgggcgac gacgagctcg tgtcggacct cttcgccctg ctctacgagg
    12121 tcgagaccga tttcacgatc ttctttcgaa acctcgcggc cgtggaaatg gaggcgctgc
    12181 ttcctgcctt ctactcgaag gctcccgaag gcatggcggg ctggctccaa cgctatgccg
    12241 caagggcgaa gcatgaaccg ggacgcgtcg agcgcatgaa ccgcgccaac ccgaagtaca
    12301 tcttccggaa ctacctggcg caggtcgcga tcgatgcgct cgcggccggc gatgcctcgg
    12361 tcatggagcg actgatgcag gtgctcgagc gcccgtacga cgagcagccc gagcaccagg
    12421 acctcgccgc gcgccgcccg gaatgggcgc gggacagggc gggctgctcc gccctgtctt
    12481 gcagctcctg agtcagtcga cgaccgccac gacctggatt tcgatcaatt gctcgggctc
    12541 gcccagctcg agcaccgcca tgttggtggt ggtcggcttg tggttgtggg gcgcgaagta
    12601 cgagttgact accgcgctcg agcggccgat gtcgcccatg cgcgggcggg tgcgaaagat
    12661 aaaaacatgc accacgtcct tcggcgaggc gcccgccgct ttcagcgttt ccatgatgcc
    12721 gtccatggtg cgccgggtct gggcttccag gtcgttcggc atgaactgca tgatctgctc
    12781 gcgcttgtgc ggatgatcgt gatacaccgg cagcgcggtc ccgcccgcga gccacagcag
    12841 cttgccgctc ttgatcttga gcgccggcga gaacggcatg aacaggctct tgtcgtagtt
    12901 cggatgatgg atgacctcga gatgattccc gctttgcgct ccggcggggg acatcgcgat
    12961 cgacagcgcg gcgaaactca gtacggccag cggcttcatg attttctccc ttagggtgga
    13021 cgacggaagc accgtcccga ttatcccgcc ttgcggaagg tgaggttgat ccgctgctcg
    13081 ccggtcaggg gatggaaccc cggcttcagc ggcagcacgc cgtggtagcg catccgcgct
    13141 tcgcctccca gcaccaccac gtcgccgtgc tcgagcagca cgcgctcggc cttgtcggtc
    13201 ctcttcagcc cgccgaacag gaaaaccgct gcgacgccga acgaaacgga cacgatcggc
    13261 acggcgaaat ccccttcgtc ccggtgcagc gatagtctgg cgccgggctc atagcggttg
    13321 atcaggcaag cgtcgggaac gaagccgcga aagccggcat gcgcggctgc ttcccgcgcc
    13381 aatcccagaa agctgtcggg catcggcggc cagcgcctgc cgctctcggg atcgttcgca
    13441 tcgtagcggt agccggcccg gtcgctgacc cagccaagcg gtccgcaatt ggtcatcgcc
    13501 accgacatgc ggtatccgcc cggcgtgacg aggtggcgaa acggcgcccg cgccgagatc
    13561 tccgccaccg cctcgaggaa cgtttgctcc tcgggcagcg cgaacccgcg taatagcttc
    13621 acgccgcgtc gtggaagatg atgccggccg tatggcgatg gccggagcgc acgcggctca
    13681 cgccatggcg caggttcacg cggtaggtcc cgcgggtgcc ttgcaccggc cgatgacgca
    13741 ccgcgaagac caccgcgtcg cccttgtcga gcgtcaccac ctcggcccgg gactgcatcc
    13801 gcggccgctg ctcggcgagg acgaactcgc cgccggtgaa gtcccgcccg ggctcggaca
    13861 gcaggatggt gagctgcagg ggaaacacgt gctcgccgta cagatcctgg tgcaggcagt
    13921 tgtagtcgcc ggcgccgtac tggagcagca gcggcgtcgg ccggcgctgg cccgcggcgt
    13981 ggcagcgctt gatgaacttc gcgtgctgct ccgggaagcg cacgtcgatg cccatggccg
    14041 cgttccagcg attcgcgatg gctgccaggt gcggatagag ggcggtacgc agcctggcga
    14101 tcacgtcagg caacggatag gagaaatact tgtactcgcc tcgtccgaag ccgtgtcttg
    14161 ccatgaccac gcggctgcgg aagagcgcat cgttgacgta ggcggcggcc agctcatcgc
    14221 actcgccggg tgagatgaga ttctcgagca cggtgcagcc ttgagcgtcc agctcgaccc
    14281 cgattcgcgc ccagtcgttc atggatgcaa tctatctctg gcgggccgcc cgggcactcc
    14341 gatccttgct tttgaattgc ttgtatttcc caggcaagca gaccgcgcaa ggccggtaac
    14401 ctgcagcccg ggcatgtcga gccggccgta gatccgggtg cggcggtggc cgccaagcgt
    14461 tccgggccgg cggctcgcgt acagcttgcc gtcgggcccg agaagtgtcc gggtcaaaga
    14521 ctctcctcga ggaggcgcag cgccgccgcg gcaaaagcat gcatgttggc caggcgatcc
    14581 ttgcttccgg tcttgagcgt gatcgagatt tccttacgcc cgcagaccgc aaggcaagca
    14641 tggccggcag ggtcgccgta acggttgccg gtcgggccgg cggcaccggt ctcgccgagg
    14701 ccccaggtcg cgccgaaccg gtcgcgggcg agccgggccc ggaccagggc attttgttcg
    14761 gtggccgagc gatgtcctcc cagcaggctc tcgtcgaagt cgcgcagcgc ctcccagccc
    14821 tggcgcgtgt aaatcactat cccgccaagg caataggccg aggcccccgg tacggccagc
    14881 aaggcggccg taatcagccc gcccgccgag gattcggcca cggccaccgt ctccttccgc
    14941 gccttcagcg atgctgcgat gcgttcggcg tacaccaaaa gctctttcat ggctattctc
    15001 tagggtgagg agcagtagtt aacacccgcg gcccagcgcc cgggcgtagg ctgctgttga
    15061 ggggatgagg accaatacca cactggtaat caatccgaag ggcggctcgg gcaagaccac
    15121 gattgccacc accctcgcga gctatttcgc cgccaacggc accgcgaccg cggtgatgga
    15181 ctacgacccg cagggctcca cgatgaactg gctgcgcgtg cgcccggcgc atgcgcgcag
    15241 gatccacggc gccaacgctg cgccacagaa gaccggcttg cgcacgctcg gcatgcacgt
    15301 ccctcccggc accgagcagc tcgtcatcga cgcgcccgcc ggcgcctcgg gcctcgtcct
    15361 ccaggacatg ctgtcgcggg cccattgcat cctcatcccg gtggcgccct cgccgatcga
    15421 catccacgcc accgcgaact tcatccgcga cctgctgctc accgggaaga tccgcgccaa
    15481 caacatccgg ctcggcgtgg tcgctaaccg ggtacgcaag gcgaacccgg tctacgaacc
    15541 gctcgagcgc ttcctgaagg cgctcaacct cccgcttctc gcgcggatcg gcgattcgga
    15601 ggtgttcgtc accgccgccg agtccggcct cggcatcttc gagatggact tcgccgtgtc
    15661 ggcggccgag cgcggccagt tccagccgat cgccgaatgg gtggaccgtg tccccgtcgc
    15721 ccagacgcgc gccgttccgc tcatcgccgc cgcctcccgc tccggcggct tgcgcgtcgt
    15781 cagcagcgct ccctcgctac ccggcggttt tcgctaagca cgctgttgct tttcgccgtg
    15841 agacgtagca tgatgttccc gctgtagctc gttggataaa aacaaaataa gattcaaaca
    15901 agaattacaa gaatgagaat caacacgacg ctggtggtca accccaaggg gggttcaggg
    15961 aaaaccacca tcgcgatcaa cttggccagc gccttcgcgg ccgacaacgt tgccaccacg
    16021 ctgatggact acgacccgca agggtcgagc ctcaactggc tgcgctcacg cccgccgctg
    16081 gcgccgcgaa ttcacggcgc caacggcgcc cccggaaaat acggccagct gcgcagcttc
    16141 gacatgtacg tgccgccgga aacgcgccag ctcgtcatcg acgcgcccgc gggctcggca
    16201 ggagtcctgc tgcagcaaat ggtcgatcgc gcccactgca tcctcgttcc ggtggtgcct
    16261 tccgtgatcg acatccacgc cagcggcaac ttcatcaagg acctgctttc gatgtcccgg
    16321 gtgcgcatgg gcggcgcccg cgtcggcgtg atcgcgaaca aggtgcgcgt ttccatgccc
    16381 gcctaccagc cgctcctgcg ctttctcgat acgctccagc tgaagatgcg ggcgcgcctg
    16441 ctcgattccg acgcgtacct gaaagcggcg gaagcgggcc tctcgatctt cgaatacgag
    16501 ccgaaccaga ccgtggccga gcgcaagcag ttccagccga tcgtcgactg ggtgaccggc
    16561 gagtacgcgc ggcctcagga acttgcgggc gccgttcgcg agatcacgca catccgccgc
    16621 atcagcttct gagccaggcg agcagcgccg cgttgaactc gcggggctgc tggaccatga
    16681 tccagtgccc ggtcgggaac gcgatcacgc ggcagccggg ccgcgcagcg acgcgctcgg
    16741 cccaggcgcg cgagtgaaac atgaacggct tgcgctcgcc gtacaggaag agcatcgggc
    16801 agtgggggtc gaaggccctc aggcgcccta gcccgccggc cgcgcccagc cagcgcagcg
    16861 cgtaggggta gcccatgtgc gagccaatgg ccggagaata ggtcgggcaa ccgagcacgc
    16921 gcgccatcca gcgcgccatg gcatcgccga tcccgccgcc gatccgccac gcgagtgcga
    16981 gccagacctg gtaggcgagc accatgaacc tgccgatgaa gcccagctcg gcgcggttcc
    17041 cgcgcgagcc ggcgtccccg atgtcgacgc cgatcacccg cccgaccaac tcgggatggc
    17101 gcattgcgaa ctgatagccg aagaagcagc cccagtcgtg caggagaagg gtgatgcgct
    17161 cgcccgggca aacctgctcg accacgcgcc ggatcgttcc gagcacctcc tcgaacgtat
    17221 gcacgcgctt tgatgctgcg agctcgaacc ccggcaaggt gaagcgcacg cagcggtagc
    17281 gctccttcag ggcctcgacc tgcgcgtccc acagccggtg cgtatcgggc cagccgtgga
    17341 tcatcacgat cgccggccca acgccctcga cgaacacctc gacgccgtcg atcctcatcg
    17401 gtctttcgcc tcgatccgtg cctaaacgca caacttctta ctccggatgg tccaccgcgc
    17461 accgaggcta agtccacaat ggaagcttga acaagctatt ggtgctcgac gatgatccgg
    17521 acatgtgcgc actggtcgtg catgccgccg gctccgccgg cttcgaggcg gcctccgcca
    17581 ccaatttcga gcagttcaag actcgccttg ccgcggacac gtcggtggtc gtcctggacc
    17641 tgatgatgcc ggaggtcgac ggcatccagg tgctccggta cctgagcgag caaagctacc
    17701 cggccggcat cgtcctcatc agcggctacg accggaaggt cctgaaagtc gccgcgcagc
    17761 tcgcggcgtc gctcgggctc gatatccacg cttcgatcca gaagcccttc aaagcgaacg
    17821 agctgcgcga ggttctggca aagggcggcc aaacccggcg accgctgcgc ggcggcgcgg
    17881 ccgacagcgc gatcgacgtc gaagagctcc gccaggccat cgccggggat cagctcctgg
    17941 tctactacca gccccaggtt cacatcaaga cgcgaacgct cgccggcctc gaagcgctcg
    18001 tgcgctggca gcacccgacg cggggtctcc tgccggccac cgcattcatc gagtcgttcg
    18061 aaaccgccgg actgatcggc gagttgacgt ggctcgtggt ccgcaaggct cttgccgacc
    18121 ggaaaaagca gagctcgcag gcggcacggg tcccggtctc ggtaaacctg tcggcgctcc
    18181 tgctccggga cctcgcgctt cccgaaaagc tgctggcgct gcttgcggag cacggcgcca
    18241 cgcccgccga cctggtgctg gagatcaccg agagcggcct gatgaaagag ctgcatacct
    18301 cgctcgacat cctcgcgcgc atgcggctca agcagctgca cctgtcgatc gacgatttcg
    18361 gcaccgggta cgcaatgctg cagcagctgc agcgcgtgcc ggcgcgcgag ctgaagctcg
    18421 acatttcatt cgtgcaggcg atgctggccg acgagtcggc cgacatcatc ctgaggaaga
    18481 cgctcgagct cgcgcacgac ctggacatgt ccgtcgtcgc cgagggcgtc gaaacggtgg
    18541 agcaactcaa ccggcttgcc gaatacggct gcgacgtcgc gcagggctac ctgctcggcc
    18601 ggccaagccc caccggagcg ctgcacgaaa tctgagcgaa aggcgccgag caatcggcgt
    18661 tcccgctccc ggggcatggc acttgcacaa cgttccagta tggacaacac cgccaagcag
    18721 cacctgcttc agcgggccgc tgacctactg ggccggggtg agattgctcg ccgcctgaac
    18781 acgcccccgc cggtcctcga agcttggatc tgcggagaag ccagcatgcc agacggcaag
    18841 ttgctcgtgc tcgctgccgc gctggaggag catgcgaaaa ccggcaagta ggtggcgcta
    18901 gcaggattct ctatcgcggc ttggcatgcc ggctggcctc ctcctccgtg cgctgccaca
    18961 gctccagtgc catcggctga ttcgcttcct cgagaatgtg cccgaccagg ccaatggcgc
    19021 gcgcgatgac gcccaggccg cgcacagccc tccagtcgag cttgagctcg caggcaagcg
    19081 cgccgatcgc accggtcgcg ttcagcgtca gccccttgag cctggacacc tcctcgatca
    19141 gcttgcagta gatgccgtag tggccggtct cgcgcgcgat ctcgaagagg cgaaccgagc
    19201 gcgggtcgac cggcttgtga agcggatggc caaggccggg gatgcgcttg ttctcggcga
    19261 cgatctgctc cgcggttttg tccttggatt cggtgagaag ctttgcacag ccttccatcg
    19321 aacccacgaa gacgctgccc aatccgcaca ggcccgcggc aaccgcggcc tgcatcgctt
    19381 cgggcgcgcc gacgtaagtc atgcgcgccg ccagggcgct cggcgtgacg ccgtgctcga
    19441 ccagcgtcac gaccatcgca ttgaaaatct tcgattcgtt ggccgtgggg acgcggccgg
    19501 tgatctcgag aaaggccatg tccccgaggt tgaggtgtcc aaggatctcc ttgggaaggt
    19561 ctttaccgcg aaccacgatg cggtccacgg tagaccaggc gaggtcgctg cgaatcattt
    19621 ggatgcctcc tccttgatat gcgcatggta tgccttggcc taaggacttg gtatgttgat
    19681 gcccatggcg cagcaaacca ggccaaggca tttctcgatg atccgcggct tccacctcgc
    19741 ggacttcttc accctcgcca atgccgcctg cggcgtcggc gcgatatttc tcgcgatgct
    19801 gtacctgaaa agcggggccc aggcgcattt ctacgccgcg gccgcgatgg cgcccctcgc
    19861 gttcttcttc gactggctgg acggcaacgt ggcgcgctgg cgccatcagc actcggcgct
    19921 cggccgcgag ctcgactcgc tgtccgacgt catctccttc ggtgtcgcgc ccgccgtgct
    19981 ggcctatgcc gccggactga acggcggttg ggacgccgcg gcgctcatct actttgtgtg
    20041 ctgcggcgtg agccgcctcg cgcgcttcaa cgtgaccgcc gaaacgctcg cggccggcag
    20101 cgacagcggc aaggtcgcct acttcgaagg cacgccgatc ccgaccagcg tggtgttgac
    20161 ggcagtgctc gccttcgccg cgtggcaggg ccgcatcgga gaaaacctct acggcggcgc
    20221 ctggaacctc ggtccctggc aattccaccc gctgtcgctg cttttcatcg cctcgggcag
    20281 catgatgatc agcaagacac tgcgcattcc gaagccgtaa gcttacggtg catcgacccg
    20341 gcacaagtac tgcacccacg gatgcagcca cggcgacgcg gcgacaattt gcggcgcgag
    20401 gacggtaaga gccgcgctgc agatcagaac cgtgcctgcc acgcgacggg cggtccctcc
    20461 tgcggcaagg ctcgctgctc gtcgcgcccc gtaggcgagc gcgatcatcg tcggcgccgt
    20521 tcccatcccg aacgccagca tcgtcgccgc cgcgcgcagg gcgtcctgct cgagcgccgc
    20581 gatgaggatt acggtatacg cgaagccgca cggcatccat ccccacacca tgccgaagcc
    20641 gagcgcccgc ccgacgttgg tcacgggcag gagccgccgg ccgagcggcg cgattcgacg
    20701 ccaaagcagc tgcccggcgc gcgaacccgg gtcgcgcagc gtcccgaacg ccaccagcgc
    20761 ggccacgact agcgccacgg ccgatagcgc acgcaggccc cggcgcaccg cgtccatgtc
    20821 gagccagtcc acgaaggtgc cgaccgctcc tcccgcaagc gcgcccgcaa tcgcgtagga
    20881 agcggtccgt ccaatctgat aggaaatgat cagcggcacg cgtggctgtc catggggcga
    20941 tcttgccgac gccaggccca gcgcggacga gattccgccg cacatgacca ggcagtgccc
    21001 gctggccgcg atgccgagga tcagtgcggc gccgagcgtg agtccgccgc tcactcgcgc
    21061 tcccgcggcg catcgtcggt gtcgagaagc ggcagcagcg ccggcgtttc cagatcgtcg
    21121 aattgcccgc ggttgatcgc ccagaacagc gcgatgccgg cgccgacgag gaccaggacc
    21181 gacagcggaa tgaggaacag caggctggtc atgccagcgc gccccggcgg acgatcgctt
    21241 cacccgccac gggggtttcc tcgactgggc cgatgcgcag cgcgttcacc acgaccgcga
    21301 gcgagctggc cgacatgcca atcgcggcaa gccagggagg cacgaaaccc agggcggcga
    21361 gcggaacggt ggcgaggttg tagcagagcg cccagcgccg gttctggccc aggatcgaga
    21421 gggtttgccg cgcgaccgcc cgcgccgcgg ccagcgcgtc gagttgcccg gtgatcacga
    21481 tgtcgctcgc cgcctgcgcc gcatcggccg ccgtgcccac cgcgatcgcc acgtcggcgc
    21541 cggcaagcac cggcgcatcg ttcacaccgt ctccgacgac agcgacccgg gcgccccgtg
    21601 cgcgaagcga tgcgagaagc gcgagcttgt cggtcggaga ctggcgggct ttccacgccg
    21661 cgacgccgag acgctgcgcc acggcttcga ctcgcgatct tgcgtctccg ctcgcgatcg
    21721 tgacgtcgat gccggcgcga gcgagcgcat cgaccgctgc ccgcgccccg ggccggagac
    21781 gctcgtcgac gcggaatgcc gcgaccaggc catcggcatc cgccagcacc accgcgtcct
    21841 cgaggtcagc gggacaggat gcggggaacg attcacatcc cagcgcataa tcggcccggc
    21901 cgagccgata gcctcgcccg ccgacgacgc cgccgattcc gcggcccgcg aagctttcgc
    21961 gcgcttccac ggccggaagc gttgcgggtg tttcggcgac gagtgcgcgc gcgagcggat
    22021 gacggctccc ctgtgcgagc gccgcggcaa cggcgagggc cgcatcgcgg tcgacgccct
    22081 gcctcgtggc cgcgagaccg ggcgcaatcg ccggctccgt cagcgtgccg gtcttgtcga
    22141 acaccacgtg cgtcaccgag gcgaggtcct cgagcgcatc gggacgcacg acgagcacgc
    22201 cgcggcccgt aagcaccgcg agcgcgcgcg tcacggccgc gggcgccgcc agcgcgaacg
    22261 cgcacggaca cgcgacgacg agcaccgcca cggtcgccgc gagcgcgtgc gacggatcga
    22321 cgatcgccca tccgattgcc gtgcaaatcg cgagcaccag cacgcgcgcg acgaagcccg
    22381 ccgctgcgcg ctcgccggcg cgcgccagcc ggggtttcga gctcgccgcg cgcgtagtca
    22441 gcgcgacgat gcccgcgacc acggtatccg cgccgacacg cgtcacgcgc atggtcgcgg
    22501 gcgttcccac gacgatgctg ccggcgcaca acgcctcgcc acggcgcctg tggcggggcg
    22561 tcgattcgcc gcagagcagc gcttcgtcga cgcgacactg ctcggtttcg agcatcccat
    22621 ccgcagggac gctgccgccg tcgccgacga ccacccggtc gccgggcacg agctcgaacg
    22681 cgcccacgcg cagcagcgtc ccatcggcct gcacgcgatc ggcaaacacg ggcgtcagcc
    22741 gtgcgagcgc gtcggacagg tccccagcat ggtgacgtgc ccgcatttcg agataacggc
    22801 cggtgcagag aaagaagatg aacatgctga ccgactccaa ccagatgtcg cggccgccgg
    22861 cgaagatctc gatgacgctg cccgcgtaga tgagcgcaac ggcgagcgct accgggacgt
    22921 ccatcccgag ccggcgcgcg cttgcgaggc gaaaggcgcc cgtgaagaac ggcgccgccg
    22981 aatagaacac gaccggggtc gcggccagca gcgtcaacca gcggaaaaag tcgcgggtgg
    23041 cggcgtcggc gccgtcgaac gcgccgaacc acagcgcgct cgcgtacatc atcgcctgca
    23101 tcgcgccgaa gccggcgact gcgaggcgtt tctgcgcgtc gcgagtttcg cgccggcgcg
    23161 tatcgtcgag cgcgtcgcgg tcgagcggca gcgcgcggta accgacgcgc tcgagcgcgt
    23221 cgacgatcct ggcgagcggg agcgcctttg cgtcgaaaac gatgcgcgca cgccgcgcgc
    23281 ccgcgttgac gctgacgtcg acgacgcccg gcaggcgtcc cagcgtccgc tcgatcagcc
    23341 agcagcaagc ggtgcaatgc atgccgtcga tcagcacgat cgcctcgctg ctgccgtcgg
    23401 cgagcgggcg cacgaggtgg cgcgacagct cgggccgcag gaaggccgcc gcactcgcct
    23461 ccgactcgcg gcggtctggc gcccgggcgg ccgggcccgt gcgcaaccga tagtagtcgg
    23521 ccagtcccag gtccgcgatc cattccacta cggcgcgaca gccgttgcag cacaccgcgc
    23581 gccggatacc ggcaacgttc gcttgcggcg gatccggcgg caacgactcg ccgcaatgcc
    23641 agcaggtccc agtcacgcca gcacgatcat gacgatgcag ttgacgagcg atgccagaaa
    23701 gatcagcgcg ccgagccaga ggaccggctt cacgtcattg cgtcgggtgg gactgggcgt
    23761 acacccaggc ggcgacgagc gatgcattgt cctctcccag ccgatggcgc aacgcaggca
    23821 tgacgccatt gcggccgttc cggatggact ccacgatgtc ctcgatgctg tcgccgtaga
    23881 gccagacgcc gtccgtcaga ttcggcgcgc caagcgccgt gttgcccttg ccgtcggcgc
    23941 cgtggcacgc ggcacaagcc gcgaagccct ccttgccgaa gtgcgcgcgc agcgagtcgt
    24001 gagtccgtcc cgccagcttc agcacatagt gcgcgatgtc cctgatctcc cgctcggatt
    24061 tcccgctgcc gagcggcggc atcgtcccat tgcggccatc caggatgctc gtgaggatgg
    24121 ccttgccgtc gccgccgtag agccaatcgc ggtcggtgag gtcgggtacg ccgagcgccc
    24181 ggttgccgcg cgcgtcgcgg ccgtgacatg cagcgcagtt ctcgatgaac aacaccccgc
    24241 cgacgcgaag cgcctccggg tcggcggcga tggtctcgac cggcttgccg cggacgcgct
    24301 cgagcaaagg cgcctccaat aggcggttcg actcctgctt ttgcgcaagc tcgctccccg
    24361 atgtccagcc gagcgtgccc gcgaaagcgc cgacgccggg atagagcacg agatagccta
    24421 atccggcgat cagcgacgcc gccgagagca cgatccacca cgtcggcaag cgccgtaccc
    24481 cttcgcgcag cacgccgtgc gcccacacgt ggccgctggt gccgtcgggc agcgtcggaa
    24541 tgtcgacgcg cagtccccac aagaacagga acaccgtaat gccgaggttg atggagacga
    24601 gaacgatcac ccagttcgac cagagctcgg tcatcgctcg tcctccgcat cctccatcgg
    24661 aagcttcgcc agcgcgtcga actcgcgctt gtggaacgga agccaggccc aggcccagat
    24721 cccgatgaag accagcatca ggaccagcgt gacgaatccg atgagctgtc cccagagcgg
    24781 attcaccggg acgctcccag cccctgcaga taggcgacca gggcgtccag ctcggtcttg
    24841 ccttcgaccg ccttgggggc cgcgtcgatg tcgctctcgg aatacggatc gcccagcgcg
    24901 cgcagcgtgc gcatccgtgc cgcgatgtcg ttgccgtcga gctgcgccga tgccagccag
    24961 cgataggccg gcatgttcga ttccgggacc accgcacgcg gatcgacgag atgcaagcgc
    25021 tgccacgcat cggaatactt gccgccgacg cgcgcgagat cgggaccggt gcgtttcgag
    25081 ccccactgga acggccggtc gtacaccgat tcggcgacgg tcgagtgagc gccgtagcgc
    25141 tgcgtctcgg cgcgcagcgc gcggatcatc tgcgagtgac agaggtagca gccctcgcgc
    25201 acgtagacgt cctttcccgc gaggcgcagc gggtcgtacg gcttcacccc gggcgccgga
    25261 gcgaccgcgt gcgcctggat gaagagcggc gtgagctcgg ccagccctcc cagcgacacc
    25321 atcaccacga tcaggatgcc gagcagccct gcgttcttct cgatcgcctc gaagtacttg
    25381 tagccgcgtg ccatcgtgcg cccccgtcag ccggtggccg gcagcggcgc aggcacctgc
    25441 gccggctcgg gctccggtat cggcaccggg atcggcttga tcaggtgcgc gcgcgccgcc
    25501 gcggcggtgt gccagaggtt ccacgccatg acgaccatgc cgctcagaat gaggaggccg
    25561 gaaaaccagc gcacgccgta caggcccttg atcgcgacca ggctgtcgat gaagggatac
    25621 gtcagcgagc catcggcctc cgtcgcgcgc cacatcagcc cctgcgtgat tccggcgatc
    25681 cacatggaaa acacgtacag cagcacgccg acgacgtgca gccagaagtg cagctccatc
    25741 gcgccgtgcg agtgcatcgc gggctgcccg agcgcccgcg gcgccatcgc atagagcgat
    25801 ccgatcgtga tcatcgcgac ccatcccagg gcgcccgaat gcacgtgcgc gatcgtccac
    25861 tcggtgtagt gcgagagcgc gttgacggtc ctgatcgcca tcatcgagcc ttccagggtc
    25921 gcggcggcgt agaacaccag cgccaccacc atgaacttcg ccgccggatc ggtcttcagc
    25981 cggtgccacg agccgttgaa ggtcatcagg ccgttcgccg ccgatcccca gctcggcatc
    26041 agcaggatga gcgaaaacgc cattcccagc gactgcaccc agtcgggaag cgccgtgtac
    26101 agcaggtggt gcgggcccgc ccacatgtag atcgagatca gcgcccagaa attgacgatc
    26161 gaaaagcgat agctccacag cggctgctgc gcctggcgcg gcacgaagta gtacatcatc
    26221 ccgaggaatc ccgcggtcag gaaaaaggcg accgcgttgt ggccgtacca ccactgcacc
    26281 atggcatcga ccgagccggc atacaccgga tacgacttcg tcagcgatac cggcagcgcg
    26341 atgttgttga cgacgtgcag gagcccgacg gcgatgatga acgcggcgta gtaccagttg
    26401 gcgacgtaga tgtgccggat gcgccgccgc gcgatggtcg cgaagaacac gatgccgaag
    26461 gaaacccaca cgatggcgac ggcgatgtcg atgaaccact cgggctcggc gtattccttg
    26521 ctctgcgtga tgccgaaggg catggtcgcc atcgccagca cgcagccgag ctgccacccc
    26581 cagaacgtga aattggcgag cccgccgagc gcgaggcgtg tgtgccccgt gcgctgcacg
    26641 acgtagtagc aggtccccat cagcgccgac ccgccgaacg cgaagatcac cccgaacgta
    26701 tggttcggtc gaaggcggcc gaacgacagc cacggcacgc cgaaatcgag gccgggccag
    26761 accatctcgg ccgcgacata gacgccgatc gccattccga cgatgcccca gacggcggca
    26821 gcgagcagga acagccttac gatctcgtcg ttgtatgttt ccgctgtacg gatcgaccat
    26881 cctcccctag gaacgcgggc gacgggaatt ggaccttggt cccatacgct cgggctggcg
    26941 gcctagcgcc ttcagcatga ggggcatgct aggtcccgct gccccgcaag gtttgatgca
    27001 gatcaaagcc cctaggcagg ctccaggccg tgctttcgga tgaccggggc cgcgatacga
    27061 ttttcatgcc tatcctcccg tgagcactac acgccttcaa cgatgcggag gtcccgctcg
    27121 gcgccgccgg ccagcgcgcg cagcgcttcc ttgtagtccg cagtatcgta agccgccacg
    27181 gctgcagcga gattctcaaa ctcgatcact accgtccgct cttcgatccc gctttcgtat
    27241 accttggcca cctttgaagt tcggaccagg aatcgcccgc ctgcggcctg aatcgccgga
    27301 tacgcgagct tggtgaatgc agcgaatttc tccggactct tggtcgaacg atggcagatc
    27361 acccagtacg ctttaggcat gacgggagcg tagcgtcctt ccaggggcgc tacttcgcgg
    27421 cggccgggaa ggccggcagc ttgccgatat cgcgcatgtc gtgctggatg acgacggtgg
    27481 cgttcaggtt ctgcgcgagc ttcttcaccc gctcgagcga agccagcgtc tgcgcacggt
    27541 cggcgttgaa ccagggcacg ccgttgctct cgtagttctc gcggaagtgc gcgaggtcgc
    27601 cgatcaggat caccgggccc ttctccttga gccgcaccag caggcagctg tggccgggcg
    27661 tgtgccccgg cgtgcgcagc acgacgaccg tgccgtcgcc gaacacgtcc ttgtcgatcg
    27721 ccaacggctc gaccttgccg ccatccttgg tccagggcgt gaagccgagc gcgttcgcgc
    27781 cctgcatcgg cgggttcgcg ctgacgcctt cccagtcgcc cttgccgatc agcagcgtcg
    27841 ccttcgggaa gttgatcagc tggccggtgt ggtcgccgtg gaaatggctg atcccgacgt
    27901 acttcacctg atcgggcgta accttcagct ccttcaggcg gtcaacgatg ccgaccttcg
    27961 gtgcgttcgg gttcgatccc ggcgcaaagc ccgtgtccca gaccatgtac tcgtcaccgt
    28021 gcttgatcag gtagcagctg aacgtaaaga cgaggttcag atccttgtag gcgaaggtgt
    28081 cggagaaacg ctcgttgacg ttggtgggct tggcgcccgt gccgcagtcg attcgtgtca
    28141 gggtcacttc cggcgtttgc gccgatgccg acaaccccag caatgcgccg gcgcaaagcg
    28201 ccagccagcg ttgagtctta atggcgtttc tcctcccgtt ggatggcgaa tcatacgcag
    28261 aaattgtcgt cgattcgcga cactcctgcg cgcgccggac tgtgcatcgc tgccgcgacg
    28321 ccggcgtagc gatcgaggta tccttcgcta cgtaggggca acaagcagaa gtggaggtca
    28381 cgatgtccat ccgtcgtcat gtcagcagtg cggcccttgt tacgctattt gccgcggcag
    28441 gctgtgtctc gcagggcgag ttcgacaagc tgcagagcgc gaagaaccag gagatcgcgt
    28501 cgctgcagtc gagccgctcg acgctcgaag ggcaggtgcg caacctcgag aacgagcgtg
    28561 cgagcctgga gaaacaaaaa gacagcctga caaaagagaa ggacagcctt acccagcagg
    28621 tggcggagct cgaggcgcag cgcgcgcggc tccaggcgag cgagaagcag gttgaggcgc
    28681 gctacgacag gctgcgcgcg gacctcagcg aagaggtgaa gaagggccag ctccagatac
    28741 gcgagctcga gggcaagctg accgtcgacg tcgcggagca gctcttcttc gactccggcc
    28801 gcgcggccct caaggacacc ggcaagcagg tcctgcagaa ggtcgccgac tcgctcaaga
    28861 gctacgagga caaggcgatc cgcatcgtcg gccacaccga caacgtgccg atcagcggag
    28921 gcctgcagaa agtgtttcca tcgaactggg agctctcggc tgcgcgcgcc accacggtgg
    28981 tgcgcttcct ccaggacaac ggcatcccgc ccgagaggct cgtcgccacc ggccgcgccg
    29041 agtacgctcc ggtcgcgcca aacgacagcg ccgaaggacg gcagaagaac cggcgcatcg
    29101 aaattacgct gatcgacaag gggctggtgc agtagcgcga tcgcaccaag gcagaaagcg
    29161 gttgggagag gtcgtctccc agccgcttga tgttttcagg gaattctggc gaagaactgt
    29221 cgccgattcg cgacaactcg cgcggcgcgc cggaacatgt atcgttgccg caccaccgga
    29281 cgcccaatcc aatgatggcg caacagaacc cgcttgagcg ctacgaaatt ctcaatgagc
    29341 tgggacgcgg cgcgataggc cgcgtctacg cggcgcgcga tcgctccacg ggcacggtcg
    29401 ttgcgttgaa gaccctcgat cccgcgttat tcaacgagcc cgacgcgacc gttgccgaac
    29461 tcttcctgaa ggacgcggtc tcggccgggc gcctaaggca ccgcaatatc gtcaaggtcc
    29521 atgacgccgg tgaggctggc ggcactgcgt gcgttgcgat ggagctgctt gagggcgaga
    29581 gcctgcgcag catactcgac gccgggccgc tctcgatcgc gcgcgcgatc cagatcttcg
    29641 atgacatcgc ctccgcgctc gcctatgccc atgaggaagg cgtcgtgcac cggggcgtca
    29701 agccgtcgaa catcatcgtc ttgcgctcgg gcgttgcgaa gctcggcgat ttcggcgtcg
    29761 ggcgactcgg cgaagcagcg ctactgtccg ggcagcgggg cgactgtata aggtacatgt
    29821 cccccgagca ggtgcgcggc gacccggtcg accatcggtc cgacctattt tcgctcggcg
    29881 ccgtgtttta cgaaatgctc actcgccaag cgctcttcga gggcgactct gcgaaggaaa
    29941 tcacacagaa cattcttcat gccgagccgc cgctgccgag caaggtgaac ccgctcgttc
    30001 cattcgcact tgaccggatt gttttcagca tgctcgccga ccatccggac gaccggttcg
    30061 ccaacgcccg cgtcctgctc cgcgagttgc agggcctcga ggaagggctc gggctgggac
    30121 ccggcgcggg cgccggcgcc ggcggaccga ctgcaagcgt gcccccgatg caggatgccc
    30181 ctcggttcgc ggtcgacgag cacgatgcga ggttcaggat ggaccgcgga cccgagccgg
    30241 aacggtcctc tggatctcgg gccgcacggt tcgcggcgct agccctcctg atcgccgtgc
    30301 ttcctattgg gcttggcgtc gcctggtatt actcgcccgg ccccagcgag cctgcgccgt
    30361 ccccatcgac cgcgcctccg cctgttgcag aagcgaccaa ggaacccgcg actgcgcccg
    30421 ctgcaccgga ggaatcgccg ccgcgcgcaa tggttgatgc gagagcagag caagagccgt
    30481 caggcatcgc gtccgcgccg aacccgttgc cgccgaaacc gctggttgcg gagcccagcc
    30541 cgccagcgca agaggtgccg gcggcggcgc aaccgacggc gaaaatttcg gagccgcagc
    30601 ctggcggcac ggcgaaactc atcatcgcgg tgtcgccgca aggcgagctc tacatcgatg
    30661 gcaagcacta cggcacgaca ccgccgctca cgacgctcga tctcgagccg ggaatgcatc
    30721 gcatcgagat tcgcagcgga tcgcgaaagc cgtatctcac ctacatgacg gttcaggcgg
    30781 gtgaccagcg gcgcatccga tacgacttcg ccgcaaaggg cagccgtcca ccttcccgat
    30841 agagagaaca ccaggttcct ggctactgcg gctcgagccc gactttcttc accagcgcgg
    30901 cgtacttggc cagctcggta tggaacgcag cctgcgcctg ctcggtcgtg gtcacccgga
    30961 tcgtgttgcc ctgcttcgcc atggcttcct tgaccgccgc gtcgttgaac gccgccacga
    31021 tggcgtcgtg aaccttcttc accgtcgccg ggctcatgcc cttggggccg atcacggcga
    31081 accaggcttc gacctcgaag ttcgcgagcc cctgctcggc gaaggtagga atgtcgggcg
    31141 cggccgccgt gcgctgcgcg gggaacatgc cgatcgcgcg cagcttgccg ctcgcgatat
    31201 ggccttgcac gctcggcagt gcggcggcgg cgaaatcgac ctgtccgccg agcaggtcgg
    31261 cgaccatcgg tccgacgcct ttgtagggaa tgtgccgcac cttgatcccg gcggcctcga
    31321 ggtagagctc cacggcgagg tgcaggatgg taccgctgcc gctcgaagcg tagttgagcg
    31381 cgtccggctt gcctttgacc agcgcgacga gctccttgtg gttggtggcc tgcaccttcg
    31441 aggggttgac gacgagcacc atgggcgtcg agccgaccct ggcgattggc gtgaagtcgc
    31501 ccggcatctc gaacggcagg gacttcagca cgctcgggaa gatcaccacg ttgttggaca
    31561 cgacagcgag cgtgtggccg tcgggcgcgg agcgcgctag cgtctgcagc ccgatgacgc
    31621 cgccggcgcc cgtctggttc tcgaccacga cggcagcgcc gaacgccttg gcgaacgcag
    31681 gttgcgcggc gcgcgtaatg gcgtcgacgc cggagccggc ggcgttcggc agcacgaagc
    31741 ggaccgggcg atcctgctgc gcgagcgctc cgccggcggt gagaagggca aggacaaggg
    31801 cgtagactgt tttcatggcc gatattctac ggctcgagat cccgaagccg tagcgtagtg
    31861 gcgctccgct ccaccatcta ccgggccgat ctctcggtgt cggatctgga tcgccaggtc
    31921 tacggtcggc acgcgctgac gctcgcgcgc catccttcgg agaccgaaga gcggctgatg
    31981 gtgcggctgc tcgcgtttgc actgcattcc gatgaagcgc tcgagttcgg ctgcggcctg
    32041 tccgcggaag gcgaacccga cctcatccgg cgagatccga ccggcgcggt cgacctgtgg
    32101 gtcgaggtcg ggctgccgga cgagagagac gtgcgcaagg cgtgcggtcg ctcacgcgag
    32161 gttgccattc tcgcctatgg cgcgcgacgg gtggaaagct ggtggagcgc gacaatgccg
    32221 cggccttcat gcggcaatcg aacttgcggg tgctcacgct gactggcgag gagactgccg
    32281 cgctgcaaac gctcgcggcc cgcacgatgt ccctcacgtc caccgtgcag gaacaacacg
    32341 tctggctcgc gtcggccacg tcgaccatcg agctcgcccc ggtctgcaga acgccggacc
    32401 gcaatgaaaa aggggctgcg taaattcgca accccttcat cttgcaagaa gcggctactt
    32461 gaccttgtcg tcccccagca tcgcggtctt catgtcgatc gcggcgcctc cggcgggtgc
    32521 cgggacttcg gtgacgcgct cgtcgatgtc gtcgaactcc aggcgcagcc cgcgggagaa
    32581 ggtggcccac gccttgtaga ggcaggtgac gtaagtgagc tcgaggatct gctcgtcgtc
    32641 gaaatggcgc ttcaggcgct cgaaggtcgc gtcctgcacc cgcccgtcgg caagtacgag
    32701 ctcgtcgaca tagccgagga cggcgcgctc cttcgcatcg aaggcttccg cggaggtcca
    32761 cgccggcacc gcggccacct tctcctcgct cagcccggcg gcgcgggcga acttgctgtg
    32821 ctgggagaag acgaagcggc tgccgccggc aaacccggtg cgcaggatcg cgagctcgcg
    32881 ataga
//
LOCUS       DATBSZ010000256         5477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2440274, whole genome shotgun sequence.
ACCESSION   DATBSZ010000256 DATBSZ010000000
VERSION     DATBSZ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2440274"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VIG70_16500"
     CDS             complement(<1..1071)
                     /locus_tag="VIG70_16500"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8252199.1"
                     /translation="MKRKSGVSRRDFLKTGALAGAAAVSGGSVLTASVVSATEKSKRR
                     RKRGSLVLVNGRIHTMDDRGSVVSSVAIRRGKFVEVGHGARNISGARVIDLRGKTVVP
                     GLIESHTHFVSLANRPGYHVAQWELANNVAEVLATLSARRARGDVPVGAFITAMGAGT
                     PNIWPVRQMPTLAELDAAVPDRPVFLYQGGGGPARVNSLGMAFFATVTNPTVTVNADG
                     TIAGGNPNMANRALYHLRVRQTFEDKKRSALDAMAYSASVGITANLDQTLVAVATGTL
                     DPASLDPQPTHALFNLNHYRMYDAWLALHAEGKAFVRLQMNFLHNQGFIAALGDLSNQ
                     LPELRERLKNQFQFFGDDMLRTG"
     gene            complement(1225..1908)
                     /locus_tag="VIG70_16505"
     CDS             complement(1225..1908)
                     /locus_tag="VIG70_16505"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8252200.1"
                     /translation="MHLRSPKEAAAPTVSALAYARLRTDILRGVLVPRERLRVNDIVE
                     RYGCGAIPTREALNRLAAEHLVIHSEQRGFAVAPISAQGLRDLAKARSMLVEVAMREA
                     VLHGDAAWEERVLLSYHRLSKVRRYLSLEPRVPNPAYDDPHRAFHSALISGCGSQWVV
                     TLCERLFDHAERYRNLSRKLKVAPREDEHKKIVDAALARRLDETVALSKRHVEITAEI
                     ILKHPADID"
     gene            complement(1925..4258)
                     /locus_tag="VIG70_16510"
     CDS             complement(1925..4258)
                     /locus_tag="VIG70_16510"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEY8252201.1"
                     /translation="MYLVAFAAAVLVSTGAALAQTVPATPTVQGPITGPDPMHPGMRP
                     GPEGTNLADFGYVVDEYFLSGIAGPAGATYKVRVLVWRPADPAKFSGVVVYEPTHQGG
                     NALICQFARYGIAHRGHACVTVSAQAVVLTSTTINPANPRGIGLYIYNPERYGTLPNA
                     PGNPLLHVNNNQSNEVLAQIAWLIKSNNAASPVPSAYHVNRLVMGGTSASSGFTRSYM
                     GAVHNTVFRASDGGPIVDGFLVTATLGGAPVEVTDVPTIQVPTQFEVAGTNAYRRPDS
                     DAATPPVNLFRIYEVPGMSHNDSRDQPPEVFPGCAEPLSRYPYGASTFTALQKVIDWA
                     VAGTLPPHANYIEVNAGPPRAIVFDEQGNAKGGVRTPHLDVPVARYIAPNVNVPGTLC
                     NQSGREERLSDEVLRSLYGAFGQSAYLEKFEQRLQELIAEGWWPAEYAERYARADAKA
                     FKVLPPDLVAVAPTVSPAVLFQGSTATFSALVTNAAPDGAAGVKVRFLVDGVPIGDQT
                     VAQIAAGSNATVSAQWTATPGMHAVEVRLDPDDAIAELNEANNAAAQAFEVRNDLVAP
                     SSVAAVSPAANAAGWHRGPVTVSLSAADEAGGSGVRSITWRLNGGAAVTVNGAVASVM
                     VLNEGTNTLSYSATDNTGNAEAPKTLVVRIDTTAPAGKLHLSPSVLWPANGKLVRIRT
                     HLFVASDLSGPVSVAGPVVRSDEPQTGLGRHDVGPDWVVKHGKLYLRAERSDRGNGRV
                     YTVTYTLTDRAGNSSQVSDTVTVPLKGKGHHHDDDDD"
     gene            4479..5408
                     /locus_tag="VIG70_16515"
     CDS             4479..5408
                     /locus_tag="VIG70_16515"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8252202.1"
                     /translation="MNITSRQLKAFVLTARHQSFSRAAEQLFITQSGMSVLVRELETQ
                     LGVRLFERTTRKVALTAFGSQFLPIADRNLRELEAAAANLSRSAAAASDCLSVGAAPF
                     PAAEIMPQAISAYMALRPRLHIQMIDAERTRLIDLVQSGKIDVAVLTGVREELPGMRQ
                     TSLARFALMLICPADDPRELPLELRWSDLTGLRLIGLPPEYPIQKLVDEQLIRAGRGS
                     TADTTCNFLETQIAMVEAGAGAAVTPTSAAPACVKRKVTMHAIVDPVVWTDFCWISSR
                     SRELSPSAEDFGEFLRGYLSHIADQGPRVAAAA"
BASE COUNT          815 a         1829 c         1901 g          932 t
ORIGIN      
        1 gccggtgcgc agcatgtcat ctccgaagaa ctgaaactgg ttcttcaggc gctcgcgcaa
       61 ctcgggcagc tggttggaca ggtcgccgag cgccgcgatg aagccctggt tgtgcaggaa
      121 gttcatctgc aggcggacga aggccttgcc ttccgcatgc aaggcgagcc acgcgtcgta
      181 catgcggtag tggttgaggt tgaacagggc atgcgtcggc tgcggatcga gggaggccgg
      241 atcgagcgtc ccggtggcga ccgccaccag cgtctgatcg aggttggcgg tgatgccgac
      301 cgacgccgag taggccatcg cgtcgagcgc gctgcgcttc ttgtcctcga aggtctggcg
      361 cacgcgcagg tggtacagcg cgcggtttgc catgttggga ttgccgccgg cgatcgtgcc
      421 gtcggcattg acggtcaccg tcgggtttgt cacggtcgcg aagaacgcca tgccgagcga
      481 gttgacgcgc gccggcccgc caccgccctg gtacaggaac accggccggt cgggcacggc
      541 ggcatcgagc tcggcgagcg tgggcatctg gcgcacgggc cagatgttcg gcgtgcccgc
      601 gcccatcgcg gtgatgaacg ctcccaccgg cacgtcgccg cgcgcgcggc gcgcgctgag
      661 tgtcgccagt acctcggcga cgttgttcgc gagctcccac tgcgcgacgt gatagccggg
      721 acgattggcc aggctcacga agtgggtgtg gctctcgatc aggccgggca ccacagtctt
      781 gccgcgcagg tcgatcacgc gcgcgccgct gatgttgcgc gcgccgtgtc cgacttcgac
      841 gaattttccg cgccggatcg cgaccgagga cacgacgctg ccgcggtcgt ccatggtgtg
      901 gatgcggccg ttcacgagga cgagcgagcc gcgcttgcgg cgccgcttcg acttctcggt
      961 ggcgctcacc acgctcgcgg tcagcacgct gccgccgctg acggcagccg cgccggcgag
     1021 tgctccggtt ttcaggaaat cgcgacggga aactccagac ttgcgcttca tgacgtcccc
     1081 ctccaaaagg ttcggcagaa aatatattct ctgccgccgg acgcggcttt atatatagaa
     1141 cttggtcgtg gactcacgag cgcggctcat gaatccgcgc ggtggaatcg gcccactgcg
     1201 cggcccactg cgagtgggca gcgctcagtc gatgtcggcg gggtgcttga ggatgatttc
     1261 ggcggtgatc tcgacgtggc gcttgctgag cgccacggtc tcgtcgagcc gccgggcgag
     1321 cgcggcgtcg acgatcttct tgtgctcgtc ctcgcgcggc gcgaccttga gcttgcgcga
     1381 caggttgcgg tagcgctcgg cgtggtcgaa caggcgctcg cacagggtca ccacccactg
     1441 cgagccgcag cccgagatca gcgcggagtg gaacgcgcgg tgcgggtcgt cgtaggccgg
     1501 gttcggcact ctcggctcca ggctcagata tcgccgcacc ttggagagcc ggtggtacga
     1561 gagcagcacc cgctcctccc acgccgcgtc gccgtgcagc accgcttcgc gcatcgcgac
     1621 ttccaccagc atgctgcgcg ctttcgccag gtcgcgcagc ccctgggcgc tgatcggcgc
     1681 caccgcgaag ccgcgctgct cggagtggat caccaggtgc tccgcggcga ggcggttcag
     1741 cgcctcgcgc gtcgggatcg cgccgcagcc gtagcgctcg acgatgtcgt tgacccgcag
     1801 ccgctcgcgc ggcaccagca cgccgcgcag gatgtcggtg cgcagcctcg cgtaggcgag
     1861 cgccgacacc gtcggcgcgg cggcttcctt cgggctgcga aggtgcatgg gcacgactat
     1921 acgatcagtc gtcatcgtcg tcgtgatggt ggcccttgcc cttgagcggc acggtgacgg
     1981 tgtcgctcac ctgcgagctg ttgcccgcgc ggtcggtgag cgtgtaggtc acggtgtaga
     2041 cgcgcccgtt gccgcggtcg ctgcgctcgg cgcgcagata cagcttgccg tgcttcacca
     2101 cccagtcggg accgacgtca tggcggccaa ggccggtctg cggctcgtcg cttctcacga
     2161 ccgggccggc gacgctgacc ggaccgctca gatcggaggc gacgaagaga tgcgtgcgga
     2221 tccgcaccag cttgccgttg gccggccaca gcacgctcgg cgagagatgc agcttgcccg
     2281 ccggcgcggt cgtgtcgatg cgcacgacca gcgtcttcgg ggcttccgcg ttgccggtgt
     2341 tgtcggtggc ggaatagctg agcgtatttg ttccttcgtt caagaccatg accgaagcga
     2401 cggcgccatt cacggtcacc gcggcaccgc cgttcaggcg ccaggtgatc gagcgcacgc
     2461 cgctgccgcc cgcttcatcc gcggcggaga gcgacaccgt gacgggcccg cggtgccatc
     2521 ccgccgcgtt cgccgccggc gaaaccgcgg cgacgctcga cggcgcgacg aggtcgttcc
     2581 tcacctcgaa agcttgcgcc gcggcgttgt tcgcctcgtt cagctcggcg atcgcgtcgt
     2641 cgggatcgag gcgcacttcg acggcgtgca ttcccggcgt cgcggtccat tgcgcgctga
     2701 cggtcgcgtt cgacccggcg gcgatctgcg cgaccgtctg gtcgccgatc ggcacgccgt
     2761 ccaccaggaa gcgcaccttg acgcccgctg cgccgtcggg cgccgcgttg gtcacgaggg
     2821 cgctgaaggt cgccgtggaa ccctggaaga gcaccgccgg cgacactgtg ggcgcgacgg
     2881 ccaccaggtc cggcggcagc accttgaagg ccttcgcgtc ggcgcgcgcg tagcgctcgg
     2941 cgtactcggc cggccaccag ccctcggcga tcagctcctg cagccgctgc tcgaacttct
     3001 ccaggtacgc ggactggccg aaggctccgt acaggctgcg cagcacttcg tcggacagcc
     3061 gttcctcgcg gccggactgg ttgcacagcg ttccgggcac gttcacgttc ggcgcgatgt
     3121 agcgggcgac cggaacgtcg aggtgcggcg tgcgcacgcc gcccttcgcg ttgccttgct
     3181 cgtcgaacac gatggcgcgc ggcgggcctg cattcacttc gatgtagttc gcgtgcggcg
     3241 gcagcgtccc cgccaccgcc cagtcgatca ccttctgcag cgccgtgaag gtcgacgcgc
     3301 cgtacgggta gcggctgagc ggctcggcgc agcccgggaa cacctcgggc ggctggtcgc
     3361 gcgagtcgtt gtgcgacatg cccgggactt cgtagatgcg gaagaggttt accggcggcg
     3421 tggccgcgtc gctgtcgggc cggcgatagg cgttggtgcc ggcgacttcg aactgcgtcg
     3481 gcacctggat cgtcggcacg tcggtcacct cgaccggcgc gccgccgagc gtggcggtga
     3541 cgaggaagcc gtcgacgatc ggaccgccgt cggaggcgcg gaacacggtg ttatgcaccg
     3601 cgcccatata gctccgcgtg aagcccgagg aggcggacgt accgcccatc acgagcctgt
     3661 tcacgtggta ggcggagggc accgggctcg ccgcgttgtt gctctttatg agccacgcga
     3721 tctgcgcgag cacctcgttg ctctggttgt tgttgacgtg gagcagcgga ttgccgggcg
     3781 cattgggcag cgtgccgtag cgctccgggt tgtagatata gaggccgata cccctagggt
     3841 tcgccgggtt aatcgtggtg ctggtcagga ccacggcctg cgcgcttacg gtgacgcagg
     3901 cgtggccgcg atgcgcgatg ccgtagcgcg cgaactggca aatcagcgcg ttgccgccct
     3961 ggtgagtcgg ctcgtagacc acgacgccgc tgaacttcgc cggatccgcc ggccgccaca
     4021 ccaggacgcg aaccttgtag gttgcgcccg ccggtccggc aattcccgac aagaaatatt
     4081 cgtcgacgac gtaaccgaag tccgccaggt tggtgccctc cggtcccggg cgcatacccg
     4141 ggtgcatcgg atcgggtccg gtgatcgggc cttggaccgt gggcgtggcc ggcaccgtct
     4201 gcgcgagcgc ggcaccggtc gagacgagca cagccgcggc gaacgcgacg aggtacattt
     4261 gtcctaacgt cgcagaaaca attgcaacgt ttcgaagcga aaccattatt gcgaagcagc
     4321 aatcgccctg actggtgttg ggacgcatcc ttccccccgg taattaattg ctgccaaact
     4381 gcaaaacgct cgccgcttta ggcggcgcgg agtaggattt atcacgcggt gagttctaac
     4441 tccaatagaa gtaagtcatc tattcatcac cgttagaaat gaatatcacc tctcggcagt
     4501 taaaagcctt cgtcctcacg gctcggcacc agagcttttc gcgtgccgcc gagcagttgt
     4561 tcatcacgca atcgggaatg agcgttctgg tgcgcgagct cgagacgcag ctcggcgtgc
     4621 gcctgttcga gcgcaccacg cgcaaggtcg cgctcactgc gttcgggtcg cagtttcttc
     4681 cgatcgcgga ccgcaacctg cgcgagctcg aggcggcggc ggcgaacctc agccgctcgg
     4741 ccgccgcggc gagtgattgc ttgtcggtcg gcgcggcgcc gtttccggcg gccgagatca
     4801 tgccgcaggc gatcagcgcc tacatggcgc tgcgcccgcg gctgcacatc cagatgatcg
     4861 acgccgagcg cacgcgcctc atcgacctgg tgcagtcggg aaagatcgac gtcgcggtgc
     4921 tcaccggcgt gcgcgaggag ctgcccggga tgcgccaaac ctcgctcgcg cgctttgcgc
     4981 tgatgctcat ctgccccgcc gacgacccgc gcgagctgcc gctcgagctg cgctggtccg
     5041 acctcaccgg gctgcggctg atcggcctgc cgcccgagta tccgatccag aagctggtcg
     5101 acgagcagct catccgcgcc gggcgcggca gcacggccga cacgacctgc aacttcctcg
     5161 agacgcagat cgcgatggtg gaggcgggcg cgggcgcggc agtgacgccg acttccgcgg
     5221 cgccggcgtg cgtgaagcgc aaggtgacga tgcacgcgat cgtcgatccg gtggtgtgga
     5281 ccgacttctg ctggatctcg agccgctcgc gcgagctgtc gccctcggcc gaggatttcg
     5341 gcgaattcct gcgcggctat ctcagccaca tcgccgacca ggggccgcgc gtcgccgcgg
     5401 cggcctgatt cgccgctagc gaatcggcac ctgcgagcgg aagctcgcca tgccgctcgc
     5461 gtcgaggcgc gcatgca
//
LOCUS       DATBSZ010000257         4333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_935039, whole genome shotgun sequence.
ACCESSION   DATBSZ010000257 DATBSZ010000000
VERSION     DATBSZ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935039"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..641
                     /locus_tag="VIG70_16520"
     CDS             108..641
                     /locus_tag="VIG70_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8252203.1"
                     /translation="MLKWILAAVAAAVVVFLVVVALQPSDFKVERSATMRAPAPEAFA
                     QVNDFQNWRAWSPWEKIDPALKRQYEGPKAGTGAVYAWQGNKDVGEGRMTIAESRPGE
                     LVRIKLEFFKPFAATNQADFTFKPAGDGTSVTWTMSGQNNFISKAMCLFVDMDKMVGG
                     MFEQGLAQMKTVVERRP"
     gene            642..1058
                     /locus_tag="VIG70_16525"
     CDS             642..1058
                     /locus_tag="VIG70_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8252204.1"
                     /translation="MKVEPYLMFEGRTEEALQFYQQKLGAKVEAIIRYKENPEPKYNP
                     PNSDEKVMHSLFRIGDAQIMASDGNCTGKPGFQGFALTLNAADAAEAKQRFNALAEGG
                     KVQMPLNETFFAKSFGMVADRFGVSWMVLAEPKKPA"
     gene            1055..1441
                     /locus_tag="VIG70_16530"
     CDS             1055..1441
                     /locus_tag="VIG70_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8252205.1"
                     /translation="MKKTYNGSCHCGAVRFEADIDLAQGTFKCNCSICFKSRAWLAGV
                     PASDFRLLSGEQSLADYQFGKHRIHHRFCRTCGVRPFSRSGDQLAVRVNCLDGVDSKE
                     LIGAPVKYFDMLHDNPKSPPAETRHL"
     gene            1441..2784
                     /locus_tag="VIG70_16535"
     CDS             1441..2784
                     /locus_tag="VIG70_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8252206.1"
                     /translation="MTERKRWLAFYLLCAGEFMVVIDTTIVNVALPSIQADLRFSDAS
                     LVWVVNAYMVTYGGFLLLGGRLGDLYGQRRWFLLGTALFTVASLVCALATTQWLLVVA
                     RAVQGLGGAAVSAIALSLIMNLFSEAADRAKAMGIYGFVCAGGGSVGVLAGGMLTSAL
                     SWHWVFLVNLPIGALVCAYCFVLLSPDRAPAQQRRLDVWGALTVTASSMLAVYAIVNG
                     NYPLLALAAVLLGAFLALESRVRDPLMPLRLFRVRSLAVANVIGVLWAAAMFAWFFIS
                     ALYMQLVLGYTPMQIGLAFLPANLIMAVFSIGLSAKLVMRFGLRLPIAGGMLLAAAGL
                     ALFALAPSGGSFIAHVLPGMLLLGLGCGMALNPVMLAGMSEVDASESGLASGVLNTSF
                     MMGGALGLAALASLAEAKGYHAAFAAGALFAAAAAVIAAALPRERALAAVEQGAT"
     gene            2781..3278
                     /locus_tag="VIG70_16540"
     CDS             2781..3278
                     /locus_tag="VIG70_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8252207.1"
                     /translation="MKLARNEREKQLLLVLWYDKEAEAAANFYCSIFKKSKILGITRH
                     LEGGGGAKGEVLTVQFRLGDQEYVALNGGPQFKFTEAISLMIHCKDQKEVDYYWEKLL
                     AGGGRESACGWLKDKYGLSWQVTPTILLEMISDKNRKKAQRAFDAMLQMRKIDIRKLK
                     EAYDG"
     gene            3271..3645
                     /locus_tag="VIG70_16545"
     CDS             3271..3645
                     /locus_tag="VIG70_16545"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8252208.1"
                     /translation="MGNAVIHFELMSKEPEKVSAFYSKVFDWKINHAPQINYRIVETG
                     AEGEMHGINGGIVKPDKPGPWPGNMLFYILVDDLAKYRRKVVAAGGEIHVEEHKVPGM
                     GKFALFTDPEGRMMGLWKAKNA"
     gene            3624..4028
                     /locus_tag="VIG70_16550"
     CDS             3624..4028
                     /locus_tag="VIG70_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8252209.1"
                     /translation="MESKERVKRLSVRIDRPAAEAYELLSRPESFPKWASGMGGKLRR
                     EGADWAMDTPEGTATIRFSEPNSRGVLDHQVRLPRRAPIYVPLRVVAHGDACDLVLTL
                     FRQPDMSDEKFAADAEWVMRDLHAAKRLLEGS"
     gene            4025..>4333
                     /locus_tag="VIG70_16555"
     CDS             4025..>4333
                     /locus_tag="VIG70_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEY8252210.1"
                     /translation="MSRITTGPAGVRVRVKFEGELIADTRDAIALHEGSYPPVYYLPR
                     KDVRMDRLARTSHRSHCPFKGDASYYSLKGGPENAVWSYEQPIAAVAVIKELLAFYPD
                     K"
BASE COUNT          746 a         1404 c         1468 g          715 t
ORIGIN      
        1 gccaaggcga gcgggccgag cgacgaggcc gccgcgatgc tcaagggaat ggacaaaggc
       61 tgggcgcaga ccgtcgcgcg cctggacgca cacttaagga agtgatcatg ctgaaatgga
      121 tactggccgc ggtcgcggca gccgtcgtgg tattcctcgt ggtcgtcgca ctgcagccgt
      181 cggacttcaa ggtcgagcgc agcgccacca tgcgcgcgcc cgcgcccgag gcgttcgccc
      241 aggtgaacga cttccagaac tggcgcgcgt ggtcgccgtg ggaaaagatc gacccggcgc
      301 tcaagcgcca gtacgaaggc ccgaaagccg gcaccggcgc ggtttatgca tggcagggca
      361 acaaggacgt cggcgaaggc cgcatgacga tcgccgagag ccgcccgggc gagctggtgc
      421 gcatcaagct cgagttcttc aagccgttcg ccgccaccaa ccaggccgac ttcacgttca
      481 agcccgcggg cgacggcacc tccgtcacct ggaccatgag cggccagaac aacttcatct
      541 cgaaggcgat gtgcctgttc gtcgacatgg acaagatggt cggcggcatg ttcgagcagg
      601 gactggcgca aatgaaaacc gtagtcgaaa ggagaccgta aatgaaagtc gagccctatc
      661 tgatgttcga aggccgcacc gaggaagcgc tccagttcta ccagcagaag ctgggtgcga
      721 aggtcgaagc gatcattcgc tacaaggaaa atccggagcc gaagtacaac ccgccgaact
      781 ccgacgagaa ggtgatgcac tcccttttcc gcatcggcga tgcgcagatc atggcgtccg
      841 acggcaactg caccggcaag ccgggcttcc agggcttcgc gctcaccctc aacgccgccg
      901 atgcggccga ggcgaagcag cgcttcaacg cgctcgccga aggcggaaag gtgcagatgc
      961 cgctcaacga gactttcttc gccaagagct tcggcatggt cgcggaccgc ttcggcgtgt
     1021 cgtggatggt gctggcggaa ccgaagaagc ccgcgtgaag aagacctaca acggcagttg
     1081 ccactgcggc gcggtgcgct tcgaggccga cattgacctc gcgcagggca ccttcaagtg
     1141 caactgctcg atctgcttca agtcgcgggc gtggctggcg ggtgttccgg caagcgactt
     1201 tcgcctgctc tccggcgagc agtcgcttgc cgactaccag ttcggcaagc accgcatcca
     1261 ccaccgcttc tgcaggacct gcggcgtacg ccctttctcc cgctccggcg accagctcgc
     1321 ggtgcgcgtg aactgcctcg acggcgtgga ctcgaaggag ctgatcggtg cgccggtgaa
     1381 gtacttcgac atgctgcacg acaaccccaa gtcgccgccg gcggagacgc gccacctgtg
     1441 atgaccgaaa ggaagcgctg gctggcgttc tacctgctgt gcgcgggcga gttcatggtc
     1501 gtgatcgaca ccacgatcgt caacgtcgcc ctgccgtcga tccaggccga cctgcggttc
     1561 agcgacgcgt cgctggtctg ggtggtcaac gcctatatgg tcacttatgg cggcttcctg
     1621 ctgctcggcg ggcggctcgg cgacctgtac ggccagcgcc gctggttcct gctcggcacc
     1681 gccctcttca ccgtcgcctc gctggtgtgc gcgctggcca ctacgcagtg gctgctggtc
     1741 gttgcgcgcg cggtgcaagg cctgggcggc gccgcggtgt cggcgatcgc gctctcgctc
     1801 atcatgaacc tgttcagcga ggccgccgac cgggcgaaag cgatggggat ctacggcttc
     1861 gtctgtgccg gcggcggcag cgtcggcgtg ctcgcgggcg gcatgctcac cagcgcgctg
     1921 agctggcact gggtgttcct ggtcaacctg ccgatcggcg cgctggtgtg cgcgtattgc
     1981 ttcgttctgc tttcgccgga ccgcgcgccg gcgcagcagc ggcgcctcga cgtctggggc
     2041 gcgctcacgg tgacggcttc gtcgatgctc gcggtgtacg cgatcgtgaa cggcaactat
     2101 ccgctgctcg cgctcgcggc ggtcctgctc ggcgcgtttc tcgcgctcga gtcgcgcgtg
     2161 cgcgacccgc tgatgccgct gcgcctgttc cgcgtgcgca gcctcgcggt ggcgaacgta
     2221 atcggcgtgc tgtgggcggc ggcgatgttc gcctggttct tcatctccgc gctctacatg
     2281 cagctcgtgc tcggctacac gccgatgcag atcggcctcg ccttcctgcc ggccaacctg
     2341 atcatggcgg tgttctcgat cggcctgtcg gcgaagctgg tgatgcgctt cggcctgcgg
     2401 ctgccgatcg cgggcggcat gctgctcgcc gccgcggggc tcgcgctgtt cgcgctcgct
     2461 ccgagcggcg gcagcttcat cgcccacgtc ctgccgggca tgctgctgct gggcctgggc
     2521 tgcggcatgg cgctcaaccc ggtgatgctc gccggcatga gcgaggtcga tgcgagcgag
     2581 tccggcctcg cctcgggcgt gctcaacacc tccttcatga tgggcggcgc gctcggcctt
     2641 gccgccctcg cgagcctcgc cgaagccaag gggtatcacg ccgcctttgc cgccggcgcg
     2701 ctgttcgccg ccgctgccgc cgtgatcgcc gcagccctgc cgcgcgaacg cgcgctggcc
     2761 gcggtcgaac agggagcgac gtgaagctcg cgcgaaacga gcgcgagaaa cagctgctcc
     2821 tcgtcctctg gtacgacaag gaagcggaag cggccgcgaa cttctattgc tcgatcttca
     2881 agaagtcgaa gatcctcggc atcacgcgcc acctcgaagg cggcgggggc gccaagggcg
     2941 aggtgctgac ggtgcagttc cggctcggcg accaggagta cgtcgcgctg aacggcgggc
     3001 cgcagttcaa gttcaccgag gcgatctcgc tcatgatcca ctgcaaggac cagaaggagg
     3061 tcgactacta ctgggagaag ctgctcgccg gcggcgggcg ggaaagcgcg tgcggctggc
     3121 tgaaagacaa gtacggactg tcgtggcagg tgactccgac gatcctcctc gagatgatct
     3181 ccgacaagaa ccgcaagaag gcgcagcgcg cgttcgacgc gatgttgcag atgcgcaaga
     3241 tcgatatccg gaaattgaag gaggcctacg atgggtaacg cagtgattca cttcgagttg
     3301 atgtccaagg agccggagaa ggtgtccgcg ttctattcga aggtcttcga ctggaagatc
     3361 aaccacgcgc cgcagatcaa ctaccgcatc gtggagactg gcgctgaggg tgagatgcac
     3421 ggcatcaacg gcggcatcgt caagcccgac aagccgggcc cgtggccggg caacatgctg
     3481 ttctacatcc tggtcgacga cctggcgaag taccgcagga aagtcgtcgc cgccggcggc
     3541 gagatccacg tcgaggagca caaggttccg gggatgggca agttcgcgct gttcaccgac
     3601 cccgagggcc ggatgatggg cctatggaaa gcaaagaacg cgtgaagcgc ctgagcgtgc
     3661 ggatcgatcg ccccgcggcc gaggcctacg agctcctctc gcggcccgaa agcttcccca
     3721 agtgggcctc cgggatgggc ggcaagctgc ggcgcgaagg cgcggactgg gcgatggaca
     3781 cgcccgaagg aacggccacc atccgcttct cggagccgaa ctcccgcggc gtgctcgatc
     3841 accaggtgag gctgccgcgc cgcgcgccga tctacgtgcc gctgcgcgtg gtggcgcacg
     3901 gcgatgcgtg cgacctggtc ctgacgctgt ttcgccagcc ggacatgtcc gacgaaaaat
     3961 tcgccgccga cgccgagtgg gtcatgcgcg acctgcacgc cgccaagcgg ctgctggagg
     4021 ggtcatgagc cgaatcacga caggaccggc aggcgtgcgg gtgcgcgtga agttcgaggg
     4081 cgagctgatt gcggacaccc gagatgcaat cgcgctccac gagggcagct atccgcccgt
     4141 gtactacctg ccgcgcaagg acgttaggat ggaccgcctc gcgcgcacgt cgcaccgctc
     4201 ccattgcccc ttcaagggag acgcgtcgta ctactcgctg aagggcggcc ccgagaacgc
     4261 cgtctggagc tacgagcagc cgatcgcagc cgtagcggtg atcaaggagc tgctcgcgtt
     4321 ctacccggac aag
//
LOCUS       DATBSZ010000258         6658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1619352, whole genome shotgun sequence.
ACCESSION   DATBSZ010000258 DATBSZ010000000
VERSION     DATBSZ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619352"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VIG70_16560"
     CDS             complement(<1..514)
                     /locus_tag="VIG70_16560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8252211.1"
                     /translation="MLAATEVVKSFGGFQALAKCTLAIERGTVTGIIGPNGAGKSTLF
                     EIITGFYRPDEGEVRLDGARLTGLSPDAVCRRGIARTFQKLRPFQGLTVLDNVMVGAL
                     TRTRDVKHAREQAREILAKVGLSEKVDAYARTLSTGQRKRLELARALATQPRVLLLDE
                     VTGGVDQRTIP"
     gene            complement(548..1639)
                     /locus_tag="VIG70_16565"
     CDS             complement(548..1639)
                     /locus_tag="VIG70_16565"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY8252212.1"
                     /translation="MPCDTFVENAWYVAGRSQEFQVDELQGRKIANRPVLVWRTRDGK
                     VGAFDDRCVHKRMPLSEGRVLADGMLECAYHGFCYDAAGKCQRIPSQLDRPIPTKARL
                     TPLPSLEQDGLVWVWPGDPAKIGAVQAPRTPEISGEAWETHMFDPVHVPANYMLLIEN
                     LMDITHFYPLHEGNIGDIEQSKIPIELVEETLWGTPTVKTIRRVQGYKQPPFMFDWWG
                     VPVVDRWHTHHMVAPGLTRVELRCAPPGKLGQAEHEFGYVIHHSHTPVDPTNHVWRLW
                     VSTRRGKPSASDPSKTCTQRIAETFPTVMQEDLWALERQQRMFDYPDDDYTEVFLRSD
                     QALLRARKILAELEKGNRGKISAPARQAA"
     gene            complement(1669..3513)
                     /gene="aprA"
                     /locus_tag="VIG70_16570"
     CDS             complement(1669..3513)
                     /gene="aprA"
                     /locus_tag="VIG70_16570"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA];
                     GO_process: GO:0019424 - sulfide oxidation, using siroheme
                     sulfite reductase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit alpha"
                     /protein_id="HEY8252213.1"
                     /translation="MGLFGGRKTVLVDADILVIGGGMAGCGATYESRYWGRDLKIVCV
                     EKANIERSGAVAQGLYAINCYMGMQWGENQPEDHVRYARNDLMGLVREDLGYDIARHV
                     DSTVHKFEEWGLPIMRDPKTGRYQREGKWQIMIHGESYKPIVAEAARKAATEVYNRIM
                     VTHLLMDTKQANRVGGAVGFNVRTGDFYVFRAKAVIVSAGGASHIFKPRAVGEGMGRT
                     WYAPWSSASAYALPIQVGAKMTQMENRIVLTRFKDGYGPVGAYFLHLKTYTQNGYGEE
                     YEKRWYEDTKALVGDYIDRHPVPTCLRNHAILREVAAGRGPIRMVTKEAFQDPHKETV
                     GWENFLGMTIGQAVVWASQNIDPKFINPELTTSEPYVMGSHATCSGAWASGPEDYAPS
                     EYQWGYNRMMTVDGLFGAGDTIGGTAHKFSSGSFTEGRIAAKAAVKYVMEKGRDQPQV
                     SEQEVRDLEKTIYQPLENYRVGRNEIVAGTVSPSYLLPIHGLQRLEKIMDEYVGGIST
                     NYMTNGVLLQRGLELLMMMKEDMEHMGAEDLHQLQRTWELHHRLLASESVTQHTLFRE
                     ETRWPGYYYRGDHMKLDDANWHCFTLSRYDRTTGNWAMEKAPVYHIID"
     gene            complement(3513..3977)
                     /gene="aprB"
                     /locus_tag="VIG70_16575"
     CDS             complement(3513..3977)
                     /gene="aprB"
                     /locus_tag="VIG70_16575"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA];
                     GO_process: GO:0019424 - sulfide oxidation, using siroheme
                     sulfite reductase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit beta"
                     /protein_id="HEY8252214.1"
                     /translation="MPTFVYMTRCDGCGHCVDICPSDIMHIDKTYRRAYNIEPNMCWE
                     CYSCVKACPQNAIDARGYADFAPLGHSVRVLREEKKGTISWKLKFRDGREKNFESPIR
                     TTDWGTIKGPAEYPAPRPEDFASQELAHEPEALNTNKGLPALRRDQLKQGLV"
     gene            complement(3997..4764)
                     /locus_tag="VIG70_16580"
     CDS             complement(3997..4764)
                     /locus_tag="VIG70_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252215.1"
                     /translation="MFAINPFAELSAFIPPAVMQTYVVIMAVLVAVGTLFDIVHKGSA
                     KYFFANWRKMKGKAKEVGSGELVSIAIKTGVVDVLASGEFCNARRRVAHLLTMYGFVL
                     HVVSTVVMVFGLSMDSLWPHLWWIGGLMILVGGYWFWFFIRADVAAEGNSPWRVMHAD
                     LFILSLLASVTLGLVWAFLQAGTRPGAGLFFALYILATTVLFASVPWSKFAHMFFKPA
                     AAFEKRVSYANGTRSNLPAPADKPERFGSARTPPQHY"
     gene            4900..5745
                     /locus_tag="VIG70_16585"
     CDS             4900..5745
                     /locus_tag="VIG70_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8252216.1"
                     /translation="MAWWIWPLALFAVTFALGILAVLAGVGGGVLFVPIVAGFFPFHI
                     DFVRCAGLLLALSGALSAAPGMLRSGFSSLRLAVPLALVGSITSAAGALLGLALPDRV
                     IHLALGATILGIAALMWSAPRPAGAQGVAPDPLATALAMHGIYHDPATGRDVEWRAGR
                     APQGLVLFAGIGLLAGMFGLGAGWANVPVLHLVMGAPLKLSIGTSGLVLSVVDSSAAW
                     IYVNRGAVLPMLVAPSLIGVILGARIGVRLLRIAHAGVLRRLVIALLLLAGARALLKG
                     LGVWA"
     gene            5778..6152
                     /locus_tag="VIG70_16590"
     CDS             5778..6152
                     /locus_tag="VIG70_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252217.1"
                     /translation="MYARWASWGTRIGLALLVCSFLAYALALLEPLVPLERLPAVWAL
                     PVERYVALTGAPTGWGWLATLRYGDYLNLLGIALLASVTMLCYARLLVSFLRRRDRLQ
                     AALAAAQIAVLLAAASGLVGGH"
     gene            complement(6135..>6658)
                     /locus_tag="VIG70_16595"
     CDS             complement(6135..>6658)
                     /locus_tag="VIG70_16595"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY8252218.1"
                     /translation="KICIVSDSHDRADPLAGAVREAAAQGAKAVIHCGDVIGTQTLRA
                     AMAVGLPMHVIHGNNLGDPISLSRWARESNGKLQYHGPDARLELGGRKIFVVHYPEYG
                     YAMACTGDWDLVCCGHSHVAGIDEVSNVKGGKTWLVNPGTVAGLAAPATWMFADLAAM
                     RFEVRAVNSVAAH"
BASE COUNT          975 a         2246 c         2205 g         1232 t
ORIGIN      
        1 cggggatcgt gcgctgatcg acgccgcccg tgacctcatc gagcaacagc acgcgcggct
       61 gcgtcgcgag ggcgcgcgcc agctccagcc gcttgcgctg ccccgtcgac agcgtgcgcg
      121 cgtaggcgtc gaccttctcg ctgagcccga ccttcgcgag gatctcgcgc gcctgctcgc
      181 gggcgtgctt cacgtcgcgc gtgcgcgtca gcgcgccgac catcacgttg tcgagcacgg
      241 tcaggccctg gaagggccgc agcttctgaa aggtccgggc gatcccgcgg cggcacaccg
      301 cgtcgggcga cagcccggtc agccgtgcgc cgtcgaggcg gacctcgccc tcgtcgggcc
      361 ggtagaagcc cgtgatgatc tcgaacagcg tcgacttgcc ggcgccgttc ggcccgatga
      421 tgccggtgac ggtgccgcgc tcgatcgcca gcgtgcattt cgcaagcgcc tgaaaaccgc
      481 cgaacgactt gacgacttcc gtcgcggcaa gcatgccgat cggtctcccc ctccggtgag
      541 gcttttttta ggcggcttgc cgggcgggcg cggagatctt tccgcgattg cccttctcga
      601 gctcggcgag gatcttgcgc gcgcgcaaca gcgcctggtc cgagcgcagg aacacctcgg
      661 tgtaatcgtc atccgggtag tcgaacatgc gctgctgccg ctcgagcgcc cagagatcct
      721 cctgcatcac cgtcggaaac gtctcggcga tgcgctgcgt gcaggtcttg gaggggtccg
      781 aggccgacgg cttgccccgg cgcgtgctga cccagagccg ccagacgtga ttggtgggat
      841 cgacgggggt gtgcgagtga tggatcacgt agccgaactc gtgctcggcc tggccgagct
      901 ttcccggcgg cgcgcagcgc agctcgacgc gcgtcaatcc cggcgcgacc atgtgatgcg
      961 tgtgccagcg gtcgaccacc ggtacgcccc accagtcgaa catgaacggc ggctgcttgt
     1021 agccctggac gcggcggatg gtcttcaccg tcggcgtgcc ccagagcgtt tcctcgacca
     1081 gctcgatcgg aatcttgctc tgctcgatgt cgccgatgtt gccctcgtgc agcggataga
     1141 agtgcgtgat gtccatcagg ttctcgatca gcagcatgta gttcgccggg acatgcaccg
     1201 gatcgaacat gtgcgtctcc cacgcctcgc ccgagatttc gggcgttcgc ggcgcctgca
     1261 cggctccgat cttcgccgga tcgccgggcc agacccacac caggccgtcc tgctcgagcg
     1321 acggcaacgg cgtcagcctg gccttggtcg gaatcgggcg gtcgagctgg gacgggatgc
     1381 gctggcactt tcccgcggcg tcgtagcaga acccgtggta ggcgcactcc agcatgccgt
     1441 cggcaagcac gcgcccctcc gacagcggca tgcgcttgtg cacgcaacgg tcatcgaacg
     1501 cgccgacctt gccgtcgcgc gtgcgccaga ccagcaccgg ccggttggcg atcttgcgcc
     1561 cctggagctc gtcgacctga aactcctggg accggccggc gacataccag gcattctcga
     1621 caaaagtatc gcagggcatc tgttcctccg gccataacga taatcgattc agtcgatgat
     1681 gtggtagacc ggcgccttct ccatcgccca gttgccggtc gtccggtcgt agcgggacaa
     1741 ggtgaagcaa tgccagttgg catcgtccag cttcatatga tcaccccggt agtagtaccc
     1801 cggccagcgc gtttcctcgc ggaagagcgt gtgctgcgtc accgactccg aggcgagcag
     1861 ccggtggtgc agctcccagg tgcgctgaag ctggtgcagg tcttcggcgc ccatgtgctc
     1921 catgtcctcc ttcatcatca tcagcagctc gaggccgcgc tggagcagca cgccgttcgt
     1981 catgtagttg gtgctgatgc cgccgacgta ttcgtccatg atcttctcga gccgctgcag
     2041 gccgtgaatc ggcagcaggt agctcggcga tacggtgccc gcgacgatct cgttgcggcc
     2101 gacgcggtag ttctccagcg gctggtagat ggttttctcc aggtcgcgaa cttcctgctc
     2161 gctcacctga ggctggtccc gccccttctc catcacgtac ttgaccgcgg ccttggcggc
     2221 gatgcggcct tcggtgaacg agcctgacga gaacttgtgc gcggtgccgc cgatggtgtc
     2281 gccggcgccg aacaggccgt cgaccgtcat catgcggttg tagccccact ggtattccga
     2341 gggggcatag tcttccggcc cgctcgccca cgcgccggag caggtggcgt gcgagcccat
     2401 gacgtagggc tccgacgtgg tcagctcggg gttgatgaac ttcgggtcga tgttctgcga
     2461 cgcccagacg accgcctgcc cgatcgtcat gccgaggaag ttctcccagc cgacggtctc
     2521 cttgtgcggg tcctggaacg cctccttggt caccatgcgg atcggaccgc ggccggccgc
     2581 gacttcccgc aggatggcgt ggttgcgcag gcaggtcggc accgggtggc ggtcgatgta
     2641 gtcgcccacc agcgctttgg tgtcctcgta ccaccgcttc tcgtactcct cgccatagcc
     2701 gttctgcgtg tacgtcttca gatggaggaa gtacgcgccc accggtccgt agccgtcctt
     2761 gaagcgggtg aggacaatgc ggttctccat ctgcgtcatc ttggcgccga cctggatcgg
     2821 cagcgcatag gcggacgcgc tcgaccaggg cgcataccag gtccgcccca ttccctcgcc
     2881 cacggcgcgc ggcttgaaga tgtgcgacgc gccgccggcg gagacgatca ccgccttcgc
     2941 gcggaacacg tagaagtcgc ccgtgcgcac gttgaacccg acggcgccgc cgacgcggtt
     3001 cgcctgcttc gtgtccatca gcaggtgggt caccatgatc cggttgtaga cctcggtcgc
     3061 ggccttgcgc gcggcctcgg cgacgatcgg cttgtagctt tcgccgtgga tcatgatctg
     3121 ccacttgcct tcgcgctggt agcgcccggt cttcgggtcg cgcatgatcg gcaggcccca
     3181 ctcctcgaac ttgtgcaccg tggagtccac gtgccgcgcg atgtcgtagc ccaggtcctc
     3241 gcgcaccagg cccatgaggt cgttgcgcgc gtagcgcacg tggtcctcgg gctggttctc
     3301 gccccattgc atgcccatgt agcagttgat ggcgtagaga ccctgcgcga cggcgccgct
     3361 cctctcgatg ttcgctttct cgacgcagac gatcttcagg tcgcgtcccc agtagcgcga
     3421 ttcgtaggtc gcgccgcaac cggccatgcc gccgccgatg accaggatgt cggcgtcgac
     3481 caaaaccgtt ttcctgccgc cgaacaggcc catcagacga gtccctgctt gagctgatca
     3541 cgacgcaacg cgggcaatcc cttgttggtg ttgagcgctt cgggctcgtg cgcgagctcc
     3601 tggcttgcga aatcctcggg gcggggcgcg gggtactcgg ccggcccctt gatcgttccc
     3661 cagtcggtgg tgcggatcgg cgactcgaag ttcttctcgc ggccgtcgcg gaacttaagc
     3721 ttccacgaaa tggtgccctt cttctcctcg cgcagcacgc gcacgctgtg ccccagcggg
     3781 gcgaagtcgg cgtagccgcg cgcatcgatc gcgttctgcg ggcaggcctt cacgcaggag
     3841 tagcactccc agcacatgtt cggctcgatg ttgtaggcgc gccggtaggt cttgtcgatg
     3901 tgcatgatgt ccgacgggca gatgtcgacg cagtgcccgc agccgtcgca ccgggtcatg
     3961 tagacgaagg tcggcatggt ttcgctttct cctagatcag tagtgctgcg gcggcgtgcg
     4021 agcgctgccg aaccgctcgg gcttgtcggc aggcgcgggc aggttgctcc gcgtgccgtt
     4081 cgcgtacgag actcgtttct cgaacgctgc cgcgggcttg aagaacatgt gggcgaactt
     4141 ggaccacggc accgatgcga acagcaccgt ggtggcgagg atgtacagag cgaagaacaa
     4201 gccggccccg ggacgagtgc ccgcctgcag gaaggcccag acaaggccca gcgtcacgct
     4261 ggcgagaagc gagaggatga agaggtccgc gtgcatgaca cgccacggcg agttcccttc
     4321 ggcggcgacg tcggcccgga tgaagaacca gaaccagtag ccgcccacca ggatcatcag
     4381 gccgccgatc caccagaggt gcggccacag ggaatccatc gacaggccga acaccatgac
     4441 gacggtggag acgacgtgga gcacgaagcc gtacatggtg agcaggtgcg cgacgcggcg
     4501 gcgcgcgttg cagaattctc ccgacgcgag gacgtccacg acaccggtct tgatggcgat
     4561 cgataccagc tcgccgctgc cgacctcctt cgccttgccc ttcatcttgc gccagttggc
     4621 gaagaagtac ttcgcgcttc ccttgtgcac gatgtcgaac agcgtcccca ccgcaaccag
     4681 gacggccatg atgacgacat aggtctgcat gacggccggc ggtatgaacg ccgaaagctc
     4741 ggcaaacggg ttgattgcga acatcgacgc cctcctcgcc gacgattcta agagcggggt
     4801 gggggctgcc ccacaaaaaa ggcgctatgc cgccatagaa gcggcaaatc ccgcgctgcc
     4861 gagcgtcctt ccttagaatc gggcgcgatg ctaggcgata tggcttggtg gatctggccg
     4921 ctggcgctgt tcgccgtcac gttcgcgctc ggcatcctgg cggtgctcgc gggcgtcggt
     4981 ggcggcgtgc tgttcgtccc gatcgtcgcg ggatttttcc cgttccacat cgacttcgtg
     5041 cgctgcgccg gtctgctgct cgcgctctcc ggcgcgctct ccgccgcccc cggaatgctg
     5101 cgcagcggtt tttccagcct gcggctcgcc gtgcccctcg ccttggtcgg gtcgatcacc
     5161 tccgccgcag gcgcgctgct tggcctggcc cttccggatc gagtaatcca cctcgccctc
     5221 ggtgcgacga ttcttggcat cgccgcgctc atgtggagcg cgccgcggcc ggcaggggcg
     5281 cagggcgtcg cgcccgaccc gctggcgacg gcgctggcaa tgcacggcat ctaccacgac
     5341 cctgcgacgg ggcgcgacgt cgagtggcgg gccggccgag cgccgcaggg cctggtgctg
     5401 ttcgcgggta tcggcctgct cgcggggatg ttcggcctgg gagcgggctg ggccaacgtg
     5461 ccggtgctgc acctagtcat gggcgctcct ctcaagctct cgatcggcac ttccggcctg
     5521 gtgctatcgg tggtcgacag ctcggccgcc tggatctatg tcaatcgcgg cgccgtgctg
     5581 ccgatgctcg tcgcaccttc gctcatcggc gtgatcctgg gcgcaaggat cggcgtgcgg
     5641 ctgctgcgca tcgcgcacgc gggggtgctg cgccgcctgg tgatcgcgct gctactgctt
     5701 gccggcgcgc gcgcgctcct caaaggcctg ggggtttggg cgtgatgaag ttgctgccgc
     5761 cttcgccgga gcatcggatg tacgcgcgtt gggcgagctg gggaacgcgt atcggcctcg
     5821 cgctattggt gtgctcgttc ctggcctacg cccttgccct gctcgagccg ctggttccgc
     5881 tcgagcgcct gcccgcagtg tgggcccttc cggtcgagcg ttacgtggcc ctgaccggcg
     5941 cgcccaccgg ctggggctgg ctcgcaaccc tccgttacgg ggactacctg aacctcctgg
     6001 ggatcgcgtt gctggcaagc gtgacgatgc tctgctacgc acgattgctg gtgagctttt
     6061 tgcgccggag ggatcggctg caggctgcgc ttgccgctgc gcagatcgcc gtccttcttg
     6121 ccgccgcctc ggggctagtg ggcggccact gaattcacgg cacgcacttc gaagcgcatc
     6181 gccgcgaggt cggcgaacat ccacgtcgcc ggcgcagcca gcccggccac cgtacccggg
     6241 ttgaccagcc aggtcttgcc gcccttcacg ttgctgactt cgtcgatccc ggcgacatgc
     6301 gaatggccgc agcagacgag atcccagtca cccgtgcacg ccatggcgta gccgtactcc
     6361 ggatagtgca cgacgaagat cttgcgcccg ccaagctcga ggcgcgcgtc cgggccgtgg
     6421 tactggagct tgccgttgct ttcccgcgcc cagcgcgaga gcgagatcgg gtcgcccagg
     6481 ttgttgccgt gaatgacgtg catcggcagg cccacggcca tcgccgcgcg cagcgtctgc
     6541 gtgccgatca cgtcgccgca atggatcacc gctttcgcgc cctgcgccgc cgcctcgcgc
     6601 acggcgccgg cgagcgggtc cgcccgatcg tggctgtccg agacgatgca gatcttca
//
LOCUS       DATBSZ010000259        12505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_801479, whole genome shotgun sequence.
ACCESSION   DATBSZ010000259 DATBSZ010000000
VERSION     DATBSZ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12505
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801479"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VIG70_16600"
     CDS             <1..1126
                     /locus_tag="VIG70_16600"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY8252219.1"
                     /translation="IPTRSGGTHEAGFRTGIFEALAAFMDGRGLAPKGVKLIPDDLWQ
                     RACFTLSARIVRTQFHGQTKEKLTTRHAARLLELCVKDAFELWLNEHVDEAKKIVELA
                     IEQAMARLAKGKKVERKKSSGIATLPGKLVDCASGDTSRNELFLVEGDSAGGSAKEAR
                     DKETQALLPLRGKVLNTMSKDSRTVLANKELQAIATAIGVDPHSSEEKADLGGLRYGK
                     VIVMTDADVDGGHIQALLLTFFFMHAPRLVESGHLYVAQPPLYKVEVPSQGKGKPQRR
                     LYCLDDDELEEVLNQLKKEKVKDGSWEISRFKGLGEMSPVQLWETTMNPDARRLVRME
                     FEAKNLKKVKETFEKLMDAGEAEERRNWMREHWKTVEADI"
     gene            1110..1355
                     /locus_tag="VIG70_16605"
     CDS             1110..1355
                     /locus_tag="VIG70_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252220.1"
                     /translation="MKRTSKKNKPFWERAYQGHALWLGKKKLGKVTLLAAQNLDPRGK
                     YRWEAGGRAGFADELTKAKSAVELAVATADKQMDLFR"
     gene            1360..3657
                     /gene="parC"
                     /locus_tag="VIG70_16610"
     CDS             1360..3657
                     /gene="parC"
                     /locus_tag="VIG70_16610"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEY8252221.1"
                     /translation="MDDTRTGDLFTNNGDDAARIEEHASHAYLGYAVSTVKSRALPEI
                     ADGLKPVQRRILFAMDGSTAFSKCARYVGEVLGKYHPHGDSSTYEALVHLAQPFSMRY
                     PLIDGQGNFGSRDGDSAAAYRYTEAKLSRFAELLLSEIGEGTVDFTKNYDGKFDEPVL
                     LPARLPFGLLNGSFGIPVGFSTRIPSHNLKEVAAAAAHVIKHPRAKLEDVLDLLPGPD
                     FPGGGQIISPKEEIKEAYATGRGSLLLRAKYEKETLARGQWRMVITELPHGVSVKQVM
                     EEIEALANPKSQGSKKEVSQEQRRMKQFILDQVEGVRDESDRKSKLRLVIEPRSSRQN
                     EHELMAALMVHTSLEARYPVNLTWLGRDGLPETKGIVDTLREWGEFRVETVRRRTDFR
                     LKKCEERLHIVEGRLKAYAKIDEIIKLIRASEDQAEARSNLQKRFKFSEKQSEDIVNL
                     RLGQLTKLDGVALNDERKALETERKSLKEILGDEKELKKLVVSELQDDAKRFGDERRT
                     LIKTAERAQVERTVVDEPVSVILSRKGWIRARTGHGLDVSQLSFKDGDALLQTLECKT
                     IDPVTLLAASGKSFTVAAADIPSGRGDGAHVNTLANSGSDDIVWMGSGDPAQRLLMNS
                     AAGLGFLCKLGDLATKTRQGKDFMKVDEGAAAQAPAFVKDDAAYVAALSSDARLLLFP
                     LDEVPERPNGGVGVQLLAVPEKEKLAALATTDGRSLVVSGIKRKNRATETLDAKQLSE
                     HIGKRAQRGRVCDVGFRPDRLETGV"
     gene            3641..4795
                     /locus_tag="VIG70_16615"
     CDS             3641..4795
                     /locus_tag="VIG70_16615"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8252222.1"
                     /translation="MKPGFDADLLKALRPAWIPAGILAATALLAWLLPLPASLAGLRT
                     AGPYALLLTGLGMAWRFNRGSAFVILASLLGGFAACQAFPARAVFSAVMVLVPLNALL
                     VLILPERGVRYRAAYGWLALLALEGALLAWFPDASLPTLAGRVAFAAAFAAAMWRAWP
                     DFTPLQVGNAGALAAFYIAAEWAGSPGVHAAFMSAAGAILIISLLEESHRLAFRDPLT
                     GLPGRRALEERLRSLGARYVIAMVDVDHFKKFNDAHGHDIGDQVLKLVGARLAEVGGG
                     GIAYRYGGEEFSVLFPGTGLDDALLFLETIRISIEGYRMAVRSPGRPKTAEDGSKRRG
                     AGNPDKHLSVTVSIGASEPSAQLRTPAQVIRAADEALYRAKQSGRNKLST"
     gene            4792..5193
                     /gene="arfB"
                     /locus_tag="VIG70_16620"
     CDS             4792..5193
                     /gene="arfB"
                     /locus_tag="VIG70_16620"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY8252223.1"
                     /translation="MTSLVIPEHEIEIIAVRAQGAGGQNVNKVSNAVHLRFDIRASSL
                     PADVKDRLLRTGDQRVTRTGMVVIKAQQHRSLERNRSEALARLHSLVAAAAHLPKKRR
                     PTRPTKASKVKRLESKKHHSKLKALRSRVFD"
     gene            5190..6935
                     /locus_tag="VIG70_16625"
     CDS             5190..6935
                     /locus_tag="VIG70_16625"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY8252224.1"
                     /translation="MKELLASVIAVVEAEGERLAAEFLAPEGPRGRRGNAPIDLEIEE
                     RLCEELQSLVNCPFTGEESGLHPGAEQDSLWLVDPHDGTFEFTSGKRGSAISVALLKD
                     KNPVLGVVHSPLSPDRGRDTIAWAEGAGPIQRNGKPLDVDLSHRLLGAGEFVFATGSS
                     ALRPGAWARAVAPARYIAMPSIAYRLARIAAGDGAATLSTHAVNEYDIAAGMALIRAA
                     GGVVLDADGKPVVLEGKLERRLSGCFAGAPGAAAQLAVHDWKSLENEPPQKARVSLGF
                     PRGVSEAELERAQGCLLGQVIGDSLGSLVEFKSAAEIALLFPAGVRELADGGVYHTIA
                     GQPTDDSEMALALARCLVAQKGFVPDKVLDAYREWMTTRPLDIGATTERGLLGLHATD
                     SESNGSLMRVSPLGVWAAGDPARAAAAAREDSALTHRNPVCVEACAGYAAAIATGIAG
                     ASREEMREAALAHSRGKAREAIERGNPPTDFMARMGWVLVALQNAFFHLSHSNFEEAL
                     IATVAQGGDTDTNAAICGALLGAALGKQAIPPRWVLPVLACRSTVDAGAPRPRPAAYW
                     PDDILELAEALLPRR"
     gene            7001..7603
                     /locus_tag="VIG70_16630"
     CDS             7001..7603
                     /locus_tag="VIG70_16630"
                     /inference="COORDINATES: protein motif:HMM:NF021489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEY8252225.1"
                     /translation="MLDVHTEIRVAKGGELTVTERITVEAHEKTGALQRELPPGSLVV
                     EVIRNGHPERYALDDGMRLRAGGEGLPAGRHLYQITYRCPRRLAFLHDHDALHWSLKG
                     AGRVTAEVILPASVPAREIKVEASGGSYQSFVRNGRAAFRGQGDIGIVVRFPKGVVVE
                     PAIDERARWFFSDYFGAVLVLALIALTAGFLIQARKLSRT"
     gene            7851..9248
                     /locus_tag="VIG70_16635"
     CDS             7851..9248
                     /locus_tag="VIG70_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY8252226.1"
                     /translation="MKISQFWDDAIIPSLVDYIRIPAKSPHFDKAWSKNGYIDAAVEL
                     AAGWCRKHAVPGMKLEVVRLEGRTPVLLIEIPAFQSASKETVLMYGHLDKQPEMVGWR
                     EGYGPWDPLIKDGKLYGRGGADDGYAVFCALSSIRALREDKRPHARIVVLIECCEESG
                     SYDLPAYLDMLEPRVGAPALVVGLDSGCGNYEQLWSTTSLRGLINGVLTVEVLTEGVH
                     SGDASGVVASSFRIARTLLDRIDAADTGIVKHSAFHAQVPPERAGQAKRAAQVLGEEI
                     WRKFPFVPNMQPMQADLADLVLNRTWRPFLAVTGADGLPPPASAGNVLRPKTELVLSL
                     RLPPTVKADSAAQQLKAILEADPPYGARVTFQAGHAGTGWHAPQTSKWLSGAVESASM
                     KNYGKSAMWMGEGGTIPFMAMLGIKYPQAQFLITGVLGPHSNAHGPNEFLHIDYAKRL
                     TACVADVLAAHAAKA"
     gene            9343..11088
                     /locus_tag="VIG70_16640"
     CDS             9343..11088
                     /locus_tag="VIG70_16640"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8252227.1"
                     /translation="MKSADWLTRFGAALEKGDINAAAGMFEPGGYWRDLVSFTWNVKT
                     CEGHAEIKAMLAAQLAGVKPKNFQAQTDEWFTFETAVGRGVGHLRLRGKHCFTLLTTL
                     QELKGFEEKAGVHRESGVSQGKTWYEKREQHVAELGVLHQPYVLIVGGGQGGIALAAR
                     LKRLQVPTLVVEKNERAGDSWRKRYRSLCLHDPVWYDHMPYLPFPDHWPVFSPKDKIA
                     DWLEMYARVMELDYWGSTLCKRARYDEAAGEWEVLVIHDGQPHTLRPKHLVLATGMSG
                     FPNMPAVEGVESFERSGRTIVHSSQYGGGASWKGKQCVVVGAGTSGHDICADLAEQGA
                     EQVTMIQRAPTIVARSETLMDLAWGPLYSEQALARGITTEIADLTVASVPFKVLPEMQ
                     RPVYADMRKRDAELYAGLEKAGFQYTFGEDGSGIHTLYLRRGAGYYIETGASQMIIDG
                     KIKLKSGVSLERITEKSVILSGGTELPADLIVFATGYGSMNHWARELISNAVADKVGK
                     VWGLGSATKDDPGPWVGELRNMWKPTQQPNLWFHGGNLMQSRHYSLYLALQLKARMEQ
                     IPTPVYYLEPVHHLQ"
     gene            complement(11153..11425)
                     /locus_tag="VIG70_16645"
     CDS             complement(11153..11425)
                     /locus_tag="VIG70_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252228.1"
                     /translation="MKLSTTIASVAVVLGVILTAGYANGEAAAVTESTKQTAQDKVVA
                     REMQRRAAVEEQQKRKDAYERACNKPRTDTEFRLCRAAYKNLEPAK"
     gene            complement(11550..12146)
                     /locus_tag="VIG70_16650"
     CDS             complement(11550..12146)
                     /locus_tag="VIG70_16650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY8252229.1"
                     /translation="MTLPAELLRSVSREEVNALPIRRYEGEVRVVSAPQDVAPALADI
                     LQEPVVGFDTETRPAFRPGESYSPSLAQAATGRAVYLFQVRHREAAAAVAQMLASAHV
                     VKAGVGLADDLKALKKLVEFSERSIVDLGAVATRHGLKQTGVRNLTGLFLGFRIPKGT
                     KTSNWSRSQLTSQQIAYAATDAWACRELYLRFSTLGLN"
BASE COUNT         2187 a         4193 c         4200 g         1925 t
ORIGIN      
        1 catcccgacg cgctcgggcg gcacgcacga ggcgggcttc cgcaccggca ttttcgaggc
       61 gctcgccgcc ttcatggacg ggcgcggcct cgcgccgaaa ggggtcaagc tcatccccga
      121 cgatctctgg cagcgcgcct gcttcacgct ttcggcgcgc atcgtgcgca cccagttcca
      181 cggccagacc aaggagaagc tcaccacccg ccacgccgcg cgcctgctcg agctgtgcgt
      241 caaggacgcc ttcgagctgt ggctgaacga gcatgtcgac gaggcaaaga agatcgtcga
      301 gctcgcgatc gagcaggcaa tggcgcgcct cgccaagggc aagaaggtcg agcgcaagaa
      361 gtcgagcggc atcgccacgc tgccgggcaa gctggtcgat tgcgcttccg gcgatacctc
      421 ccgcaacgag ctgttcctgg tcgagggcga ctccgccggc ggctcggcca aggaagcgcg
      481 cgacaaggag acgcaggcgc tgctgccgct gcgcggcaag gtgctgaaca ccatgtcgaa
      541 ggacagccgc acggtgctcg ccaacaagga gctgcaggcg atcgccacgg cgatcggggt
      601 cgacccgcac tcttcggaag aaaaagcgga cctcggcggc ctgagatacg gaaaagtcat
      661 cgtcatgacc gacgccgacg tcgacggcgg ccacatccag gcgctgctcc tcacgttctt
      721 cttcatgcac gcgccgcgcc tcgtcgagag cggccacctc tacgtggcgc agccgccctt
      781 gtacaaggtc gaagttccga gccagggaaa gggcaagccc cagcgccgcc tctattgcct
      841 ggacgacgat gagcttgaag aagtcttgaa ccaattgaaa aaagaaaaag taaaggacgg
      901 cagctgggaa atctcgcgct tcaaggggct gggcgagatg agcccggtgc agctctggga
      961 gacgacgatg aaccccgatg cgcggcggct ggtgcgcatg gagttcgaag cgaagaacct
     1021 gaagaaggtg aaagagacct tcgagaagct gatggacgcg ggggaggccg aggagcggcg
     1081 caactggatg cgcgaacact ggaagaccgt tgaagcggac atctaagaaa aacaagccct
     1141 tctgggaaag ggcgtaccaa ggccacgccc tgtggctcgg aaagaaaaag ctgggaaaag
     1201 tcacacttct cgccgcgcaa aacctcgatc cgcgcgggaa ataccgatgg gaagccggcg
     1261 gccgcgccgg atttgcggac gaactcacga aagcgaaatc ggccgtagaa cttgccgtcg
     1321 cgaccgcgga caaacaaatg gaccttttca ggtaacgaaa tggacgacac ccgaaccgga
     1381 gacctcttca ccaacaacgg cgatgacgcg gcgcgcatcg aggagcacgc ctcgcacgcg
     1441 tacctcggct acgcggtctc caccgtcaaa tcccgggcgc tgccggaaat cgccgatggc
     1501 ctgaagccgg tccagcgtcg gatcttgttt gcgatggatg gctcgaccgc attctcgaag
     1561 tgcgcacgct acgtcggcga agtcctgggc aagtaccacc cgcacggcga cagctccacc
     1621 tacgaggcgc tggtgcacct cgcgcagcct ttctcgatgc gctacccgct gatcgacggc
     1681 cagggcaatt tcggctcgcg tgacggcgac tccgccgcgg cctaccggta caccgaagcg
     1741 aagctttcga gatttgccga attgctgctg agcgaaatcg gggagggcac ggtcgatttc
     1801 acgaagaact acgacggcaa gttcgacgag ccggtgctgc tgcccgcgcg gctgcccttt
     1861 gggctgctga acggaagctt cggcatcccg gtcggcttct cgacgcgcat cccctcgcac
     1921 aacctgaagg aagtcgccgc cgccgcagcg cacgtcatca agcatccgcg cgccaagctc
     1981 gaggatgtgc tggacctcct gcccgggccg gatttccccg gcggcggcca gatcatctcg
     2041 ccgaaggagg agatcaagga ggcgtatgcc accggacggg ggtccttgct tctaagagcg
     2101 aaatacgaaa aggagacgct cgcgcgcggc caatggcgca tggtgatcac cgagctgccg
     2161 cacggcgtgt cggtgaagca ggtgatggag gagatcgaag cgctggcgaa cccgaagtcc
     2221 cagggaagca agaaggaggt ctcccaggag cagcgccgga tgaagcagtt catcctcgac
     2281 caggtcgagg gggtgcgcga cgagagcgac cgcaagagca agttgcgcct ggtcatcgag
     2341 cccagaagtt ccagacaaaa cgaacacgag ctgatggccg cgctcatggt gcacacgtcg
     2401 ctcgaagcgc gctacccggt caacctcacc tggcttggcc gcgacggcct gcccgagacc
     2461 aagggcatcg tcgacacgct gcgcgagtgg ggcgagttcc gtgtcgaaac ggtgcgccgg
     2521 cgcaccgatt tccgcctgaa gaagtgcgag gagcgcctgc acatcgtcga gggccggcta
     2581 aaggcctacg ccaagatcga cgagatcatc aagctgatcc gggcgagcga agaccaagcg
     2641 gaagccagat cgaacttaca aaaacgcttt aagttttctg aaaagcaatc ggaagacatc
     2701 gtcaacctgc gcctgggcca gctcaccaag ctcgacggcg tcgcgctgaa cgacgagcgc
     2761 aaggcgctcg agacggagcg caagagcctg aaggagatcc tcggcgacga gaaggagctg
     2821 aagaagctgg tggtctccga gctgcaggac gacgccaagc ggttcgggga tgagagaagg
     2881 accttgatca agaccgcaga gcgcgcgcag gtcgagcgca ccgtggtcga cgagccggtc
     2941 agcgtgatcc tgtcgcgcaa gggctggatc cgcgcgcgca ccggccacgg cctggacgtc
     3001 tcgcagctct ccttcaagga cggcgatgcg ctgctgcaga cgctcgagtg caagaccatc
     3061 gatccggtca cgctgctcgc cgcctcgggc aagagcttca ccgtcgcggc cgccgacatc
     3121 ccctcggggc gcggcgacgg cgcgcacgtc aacacgctgg cgaactcggg ctccgacgac
     3181 atcgtgtgga tgggctcggg cgacccggcg cagcgcctgc tcatgaacag tgcggcgggc
     3241 ctgggctttc tctgcaagct gggcgacctc gcgaccaaga cgcgccaggg caaggacttc
     3301 atgaaggtgg acgaaggcgc agccgcgcag gcgcctgctt tcgtgaaaga cgatgccgcg
     3361 tacgttgcgg ccttgtcctc ggatgcgagg ctgctcctct tcccgctcga cgaagtcccc
     3421 gagcggccga acggcggcgt gggcgtgcag ctcctcgccg tgccggaaaa agaaaagctc
     3481 gccgcgctcg ccacgaccga cggaagatcg ctcgtggtgt ccggcatcaa gagaaagaac
     3541 cgggccacgg agaccctgga cgcaaaacag ctctccgagc acattggcaa gcgcgcgcaa
     3601 cgggggagag tgtgcgatgt cggcttcagg ccggatcgcc ttgaaaccgg ggtttgacgc
     3661 cgacctcctg aaagccctgc gcccggcgtg gatcccggcc gggatcctcg ccgcgacggc
     3721 gctcctcgcc tggctcctgc cgctgccggc gtcgctcgcc gggctgcgca ccgcggggcc
     3781 gtacgcgctg ctcctcaccg ggctcggcat ggcgtggcgc ttcaaccgcg gcagcgcgtt
     3841 cgtgatcctc gcctcgctcc tcggcggctt cgccgcctgc caggcgttcc cggcccgggc
     3901 cgtgttctcc gccgtcatgg tgctcgtgcc gctcaacgcg ctcctcgtcc tgatccttcc
     3961 ggagcgcggt gtgcgctacc gcgcggcgta tggatggctt gcgctcctgg cgctggaagg
     4021 ggcgctgctc gcctggtttc cggatgcgag cctgccgacg ctcgccggcc gcgtggcgtt
     4081 cgccgccgcg ttcgccgcgg cgatgtggcg cgcctggccc gatttcaccc cgctgcaggt
     4141 cggcaatgcc ggcgcgctcg cggcgttcta catcgccgcc gaatgggccg gctcgcccgg
     4201 cgtgcacgcc gcgttcatgt cggccgcggg cgcgatcctc atcatctctc tgctcgagga
     4261 atcgcaccgc ctcgcgttcc gcgacccgct gaccgggctt cccggccggc gcgcgctgga
     4321 agagcgcctg cgcagcctcg gcgcccgcta cgtgatcgcg atggtcgacg tcgaccactt
     4381 caagaaattc aacgacgcgc acggccacga catcggcgac caggtgctga agctggtcgg
     4441 cgcgcgcctc gccgaggtgg gcggcggcgg catcgcgtac cgctacggcg gcgaggagtt
     4501 cagcgtgctg ttcccgggga ccggcctcga cgacgcgctg ctcttcctcg agacgatccg
     4561 catctcgatc gagggctacc gcatggcggt gcgctcgccc ggccgcccga agaccgcgga
     4621 ggacggcagc aagcgccggg gcgcaggcaa tccggacaag catctctcgg tcaccgtgtc
     4681 gatcggcgcc agcgagccga gcgcgcagct gcgcacgccc gcgcaggtga tccgggccgc
     4741 cgacgaggcg ctctaccgcg cgaagcaatc gggccgcaac aagctgagca cgtgacgagc
     4801 ctcgtcatcc ccgagcacga gatcgagatc atcgcagtgc gcgcgcaggg cgccggcggc
     4861 cagaacgtca acaaggtctc gaacgcggtc cacctgcgct tcgacatccg cgcctcgtcg
     4921 ctgcccgcgg atgtgaagga ccggctgctg cgcaccggcg accagcgcgt gacgcgcacc
     4981 gggatggtcg tgattaaggc gcagcagcac cgcagcctgg aacgaaaccg aagcgaggct
     5041 cttgcccgcc tgcattcgct cgtcgccgcc gcggcgcacc tgccgaagaa gcgcaggccg
     5101 acgcggccga cgaaggcttc gaaggtcaag agattggaat ctaaaaaaca ccactcgaaa
     5161 ttgaaagcgc tgcgctccag ggtcttcgat tgaaggagct gctcgcctcg gtgatcgccg
     5221 tggtcgaggc cgagggcgag cggctcgccg ccgagttcct ggcacccgag gggccgcgcg
     5281 gccggcgcgg caatgcgccg atcgacctcg agatcgagga gcgcctgtgc gaggagctcc
     5341 agtcgctcgt caactgtccc tttaccggcg aggagtcggg cctccacccc ggcgccgagc
     5401 aggattcgct gtggctcgtc gacccgcacg acggcacctt cgagttcacc agcggcaaac
     5461 ggggctctgc gatctcggta gctcttttga aagacaagaa tcctgtactg ggagtggtgc
     5521 atagcccgct ctcgcccgat cgcggccgcg acaccatcgc ctgggccgag ggcgccgggc
     5581 cgatccagcg caacggaaag ccgctcgacg tcgacctttc gcatcgcctg ctcggcgccg
     5641 gcgagttcgt gttcgccacc ggctcttccg cgctgcgccc gggagcgtgg gcgcgcgcgg
     5701 tggcgcccgc gcgctacatc gcgatgccga gcattgccta tcgcctggcg cgcatcgccg
     5761 cgggcgatgg cgcggcgacg ctgtcgaccc acgcggtgaa cgaatacgac atcgcggcgg
     5821 gcatggccct gatccgcgcc gcgggcgggg tcgtgctcga cgcggatgga aaacccgtcg
     5881 tcctggaagg caagctcgag cgtcgcctct ccggctgctt tgccggcgcg cccggggccg
     5941 cggcgcagct cgccgtccac gactggaagt cgctcgagaa cgagccgccg cagaaggcgc
     6001 gcgtgtcgct cggttttccg cgcggagtgt cggaggccga gctcgagcgc gcgcagggct
     6061 gcctgctcgg ccaggtgatc ggcgacagcc tgggctcgct ggtcgagttc aagagcgcgg
     6121 ccgagatcgc gctgctcttc cccgccggcg tgcgcgaact cgccgacggc ggcgtgtacc
     6181 acaccatcgc cggccagccg accgacgaca gcgagatggc gctcgcgctc gcgcgctgcc
     6241 tggtggctca gaagggattc gttccggaca aggtgctgga cgcctaccgc gaatggatga
     6301 ccacgcggcc gctggacatc ggcgccacca ccgagcgcgg gctcctcggc ctgcacgcga
     6361 ccgacagcga gtcgaacggc tcgctgatgc gcgtctcgcc gctcggcgtc tgggcggcgg
     6421 gcgacccggc gcgcgccgcc gcggccgcgc gcgaggactc tgcgctcacg caccggaacc
     6481 cggtgtgcgt cgaagcctgc gccggctatg cggctgcgat cgcgacgggt atcgccggtg
     6541 cgagccggga agagatgcgc gaagcagcac ttgcccactc gagaggcaag gcgcgcgagg
     6601 cgatcgagcg gggcaatccg ccgacggatt tcatggcgcg gatgggctgg gtgctggtcg
     6661 ctcttcaaaa tgcttttttt cacttgtcgc attcgaattt tgaagaagcg ctcatcgcca
     6721 cggtcgccca gggcggagac accgacacca acgcggcgat ctgcggcgcg ctgctcggcg
     6781 ccgcgctcgg caagcaggcg attccgccgc gctgggtgct gccggtgctc gcgtgccgct
     6841 cgaccgtcga tgccggcgcg ccgcggccgc ggccggcggc ctactggccc gacgacatcc
     6901 tcgagctggc cgaggcgctg ttgccgcgca gatgagaggg tctttaccgg taacttttct
     6961 actcctggga atttctctcg tcacgtctac atccgcccgc gtgctcgacg tccacaccga
     7021 gatccgcgtc gcgaagggcg gcgagctcac cgtcaccgag cgcatcaccg tggaggccca
     7081 cgagaagacc ggcgcgctgc agcgcgagct gccgccgggg tcgctcgtgg tcgaggtcat
     7141 ccgcaacggc cacccggagc gctacgcgct cgacgatggc atgcgcctgc gcgccggcgg
     7201 cgagggcctg ccggcgggcc ggcacctcta ccagatcacc tatcgctgcc cgcgccgcct
     7261 cgcgttcctg cacgaccacg acgccctgca ctggagcctg aagggcgcag ggcgcgtcac
     7321 cgcggaggtc atcctgccgg cgagcgttcc cgcgcgggag ataaaggtcg aggcgagcgg
     7381 cggcagttac cagagtttcg tccgtaacgg ccgcgccgcc ttccggggcc agggggacat
     7441 cggcatcgtg gtgcgcttcc cgaagggcgt ggtcgtcgag cccgccatcg acgagcgcgc
     7501 gcgctggttt ttctccgact acttcggcgc cgtgctggtg ctcgccctga tcgccctcac
     7561 ggcggggttc ttgatccagg ctagaaagtt atcgagaacc tgagggtatt gattcgccgg
     7621 cggcagcctc gtggcggtcg gccgcggcgt agccggcgga gagcagcacg atgaacacga
     7681 cggcgatcaa ggcggtagcg agagagagct gcattggatg gtccgtgttg tcttggttgc
     7741 ccctacgagc tgcgattgtg gccaccgcct gtttacgcag ctagcgtgca cggccacgtt
     7801 tcgcattaat ccgtgcagcc catgccgcgc tgtggctaaa gttgagggct atgaaaatca
     7861 gccagttctg ggacgacgcg atcatcccct cgctcgtcga ctacatccgc atccccgcca
     7921 agtcgccgca tttcgacaag gcgtggtcga aaaacggcta catcgacgcc gcggtcgagc
     7981 tcgccgccgg ctggtgccgc aagcacgcag tgccgggaat gaagctcgag gtggtgcgcc
     8041 tcgaaggccg cacgccggtg ctgctgatcg agattccggc gttccagtcc gcctcgaagg
     8101 agaccgtcct catgtacggc cacctcgaca agcagcccga gatggtcggc tggcgcgagg
     8161 gctacgggcc gtgggatccg ctgatcaagg acggcaagct ctacggccgc gggggggcgg
     8221 atgatggata cgccgtgttt tgtgcgctgt cttcaatcag agctcttcgc gaagacaagc
     8281 ggccccatgc gcgcatcgtc gtgctcatcg agtgctgcga ggagagcggc agctacgatt
     8341 tgccagcgta tttggacatg ttggaacccc gcgttggtgc gcctgcgctc gtggtgggtc
     8401 tcgactccgg ctgcggcaac tacgagcagc tctggagcac cacctcgctg cgcggcctga
     8461 tcaacggcgt gctcacggtg gaggtgctca ccgagggcgt gcactcgggc gacgcgagcg
     8521 gcgtggtcgc ctcgagcttc cgcatcgcgc gcacgctcct cgatcgaatc gacgccgccg
     8581 ataccgggat cgtcaagcac tcggctttcc acgcccaggt cccgcccgag cgcgccgggc
     8641 aggcgaagcg cgcggcgcag gtgctgggcg aggagatctg gcgcaagttc ccgttcgtgc
     8701 cgaacatgca gccgatgcag gcggatctcg ccgacctggt gctgaaccgc acctggcggc
     8761 cgttcctcgc cgtcaccggc gccgacggcc tgccgccgcc ggcgagcgcc ggcaatgtcc
     8821 tgcgcccgaa aaccgagctc gtgctctcgc tgcgcctgcc gcccaccgtg aaggccgaca
     8881 gcgccgcgca gcagctcaag gcgatcctcg aggccgaccc gccctacggc gcgcgcgtga
     8941 ccttccaggc cggccacgcc ggcaccggct ggcacgcgcc gcagacatcg aaatggctat
     9001 cgggcgcggt ggagagtgct tccatgaaaa actacggcaa gtccgcgatg tggatgggcg
     9061 agggcggcac catccccttc atggcgatgc tcgggatcaa gtacccgcag gcgcagttcc
     9121 tgatcaccgg cgtactcggc ccgcactcca acgcgcacgg cccgaacgag ttcctgcaca
     9181 tcgactacgc caagcgcctc accgcctgcg tcgcggacgt actggccgct cacgcggcga
     9241 aagcctagtt cggggacgga gccctaaccg gttagggctc cgtccccgaa ttaccgccgg
     9301 cggcggcaat cggggtttgc ggcaatcggc gacaattcgc cggtgaaatc cgcggactgg
     9361 ctgacaagat tcggtgctgc cctcgagaag ggcgacatca acgccgctgc cgggatgttc
     9421 gagcccggcg gctattggcg cgaccttgtc tccttcacct ggaacgtcaa gacctgcgaa
     9481 ggccacgcgg agatcaaggc gatgctcgca gcccagctcg ccggcgtgaa gccgaagaat
     9541 tttcaggccc agaccgacga gtggttcacc ttcgaaaccg cggtcggccg cggcgtcggg
     9601 cacctgcggc tgcgcgggaa gcattgcttc acgctcctca ccacgctgca ggagctgaaa
     9661 ggcttcgaag agaaagcggg cgtgcaccgc gagagcggcg tttcccaggg aaaaacctgg
     9721 tacgaaaagc gcgagcagca cgtcgccgag ctcggcgtgc tgcatcagcc ttacgtgctg
     9781 atcgtgggcg gcggccaggg cggcatcgcg ctcgctgcgc gcctgaagcg cctgcaggtg
     9841 cccacgctgg tcgtcgagaa gaacgagcgc gccggcgatt cctggaggaa gcgctacagg
     9901 tcgctctgcc tgcacgaccc ggtgtggtac gaccacatgc cttacctgcc tttcccggac
     9961 cactggccgg tgttctcgcc gaaggacaag atcgccgact ggctggagat gtacgcgcgg
    10021 gtgatggagc tcgactattg gggctcgacc ctgtgcaagc gcgcgcgcta cgacgaggcc
    10081 gccggcgaat gggaggtgct ggtcatccac gacggccagc cgcacacgct gcgcccgaag
    10141 cacctcgtgc tcgccaccgg catgtccggc tttccgaaca tgcccgcagt cgaaggggtg
    10201 gaaagtttcg agcgctccgg aagaaccatt gttcactcaa gccaatacgg cggcggcgcg
    10261 tcgtggaagg gcaagcagtg cgtggtggtc ggcgcgggca cttccgggca cgacatctgc
    10321 gccgacctcg cggagcaggg cgccgagcag gtgaccatga tccagcgcgc gccgaccatc
    10381 gtcgcgcgct ccgagacgct gatggacctc gcctggggcc cgctctactc cgagcaggcg
    10441 ctcgcgcgcg gcatcaccac cgagatcgcc gacctcacgg ttgcgtcggt tcctttcaag
    10501 gttcttccag aaatgcaaag gcccgtctat gccgacatgc gcaagcgaga cgccgagctc
    10561 tacgctggcc tggagaaagc cggcttccag tacaccttcg gcgaggacgg ctcgggaatc
    10621 cacacgctct acctgcgccg cggcgccggc tactacatcg agaccggcgc ctcgcagatg
    10681 atcatcgacg gcaagatcaa gctgaagtcg ggcgtcagcc tggagcgaat taccgagaag
    10741 tcagtaatcc tctccggcgg caccgagctc cccgcggacc tcatcgtctt cgcgaccggc
    10801 tacggctcga tgaaccactg ggcgcgggag ctgatcagca acgcggtcgc ggacaaggtc
    10861 ggcaaggtct ggggcctggg ctcggccacc aaggacgacc ccggcccgtg ggtgggcgag
    10921 ctgcgcaaca tgtggaagcc gacgcagcag ccgaacctgt ggttccacgg cgggaacctg
    10981 atgcagtcgc ggcactactc gctctacctc gcgctgcagc tcaaggcgcg catggagcag
    11041 atcccgacgc cggtgtacta cctcgagccg gtccaccacc tgcaatgaaa gacgtacaca
    11101 aaaaagggcg gcgcgctcag gggggagacg cgccgctagg ggaacgtttt tcctactttg
    11161 ccggttccag gttcttgtac gcagcccggc agagtcggaa ctcggtgtcg gtccgcggct
    11221 tgttgcacgc gcgctcgtac gcgtccttgc gcttctgctg ctcctcgacc gcggcccggc
    11281 gctgcatctc gcgcgccacg accttgtcct gcgcggtctg tttcgtcgat tccgtcaccg
    11341 cggcggcttc gccgttcgcg taacccgcag taaggatgac gccgaggacg acagctaccg
    11401 atgcgatcgt tgttgatagt ttcactggtg gttcctcccc cgctgctcgt cccccaacga
    11461 gccaagtctt tcattcgctg acaggttcgt cgtggcactg catcacgagg gtcgatcccg
    11521 cactgcggca atctgccaac aactgcgcgc tagttgagtc cgagcgtgga aaaccgcagg
    11581 tagagctcgc ggcaggccca cgcatcggtc gccgcatagg cgatctgctg cgaggtgagc
    11641 tgcgagcgcg accagttgga ggtcttggtg cccttgggaa tgcgaaagcc gaggaacagg
    11701 cccgtgaggt tgcgcacgcc cgtctgcttg aggccgtggc gcgtcgccac tgcgccgagg
    11761 tcgacgatcg agcgctcgga aaattcgacg agcttcttga gcgctttcag atcgtccgcc
    11821 aggcctaccc ccgccttcac cacatgcgcc gaagcgagca tctgcgccac cgccgcggcg
    11881 gcctcgcggt gccgcacctg gaagagatac accgcgcgtc cagttgctgc ctgcgcgagg
    11941 ctcggggaat agctctcgcc cggcctgaag gcgggccgcg tctcggtatc gaagccgacc
    12001 accggctcct gcaggatgtc cgcgagcgcc ggcgccacgt cctgcggcgc cgagaccacg
    12061 cgcacctcgc cctcgtatcg gcggatcggc agcgcgttca cttcctcgcg cgagacgctg
    12121 cgcaacagct ctgccggcag ggtcataggg gccgaagagt agttcgaatg gcgattgact
    12181 gcccgatgaa taggagtagg ctggaatcga gtccgcagac ggccgccaaa ggcagcaggc
    12241 aacacggcaa gaaccatggg attcccggca actcgtcggg atacctggcc caggcgggct
    12301 ctccgcggtt tcaccgccgc tgtaggtacc aggcgcccag ctaggcgccg cccgagtgtt
    12361 cgggcaaccc aaggccccgc ccccgtgcgg ggcctttcat ttgtgcccct ctcccgctcg
    12421 gctaagctag gccgcgatgc agcgcctggt tctcgatacc aacgtctggc tggattggct
    12481 ggtgttcggc gagcccgcgc tcgcg
//
LOCUS       DATBSZ010000260        14602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2166226, whole genome shotgun sequence.
ACCESSION   DATBSZ010000260 DATBSZ010000000
VERSION     DATBSZ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14602
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166226"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..107
                     /locus_tag="VIG70_16655"
     CDS             <1..107
                     /locus_tag="VIG70_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8252230.1"
                     /translation="RELERRYLDMRKTTIYAGSNEIQRNIIAKMTLGL"
     gene            104..1081
                     /locus_tag="VIG70_16660"
     CDS             104..1081
                     /locus_tag="VIG70_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8252231.1"
                     /translation="MNFDYNEEQQLLADSVRRFLQKDYDFEARKQIVASPAGWSERVW
                     ATFAEMGLTGLPISPDYGGFGGGAVDLMSVMEAFGEALVVEPYLPTLLAAAAIAKAGD
                     EKQKQAVLPLVAQGKLRLALATGERGVIGAPVADRIVFFRQGKLQAVERSSLQLKAYR
                     TLDDLAAADIAFQAESSNPVLDEVLDFGSALACAEAVGAMKFACDTTLEYLKTRRQFG
                     VPIGAFQALQHRMVDMFISYEQAKSMACLACSKVDQSESAAERARAVSAARIKIADSA
                     RHISQESVQLHGGMGMSEELKVSHTFRRLTVLQRRFGDVDRHLERFASL"
     gene            1089..1649
                     /locus_tag="VIG70_16665"
     CDS             1089..1649
                     /locus_tag="VIG70_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252232.1"
                     /translation="MVIVLVGCAQQPAKQDERKAPPEALPPLPATYVNDSDCPGCLEV
                     TLTLRPDGSYLVRDRLGASEFYDFGRWKYSDGTLELAGGRDAPRRYPLKELRKTPQVE
                     ALRGPFRMVGLYDGAGFKECRTGLTWQLADTRAAENLKGDFLKGQGNAVLAVIDAQFE
                     GAPEMLRVLRPASVLDARTCPAHLSG"
     gene            1661..2644
                     /locus_tag="VIG70_16670"
     CDS             1661..2644
                     /locus_tag="VIG70_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY8252233.1"
                     /translation="MENQRIVLASRPEGWVTLDNFRLEKGPLPKIGDGEVLVKNLWLS
                     LDPYMRGRMSDTKSYVKGVDLGEVMVGQTAGEVLESKNPAFKKGDQVLTQLGWQLYGV
                     TKEVMKVDGSRAPLSYYLGVLGMPGMTAYFGLKEIGQPKAGQTVVVSAASGAVGSVVG
                     QLAKLLGCRAVGIAGGREKCDYVTRELGFDACIDYKAGKLRDELKELKGIDVYFDNVG
                     GEILDLVLTRMNLFGRIVVCGMIADYNATEPYRFRNLRAVLVNRLKIQGMIVFDWKER
                     YGEALKALGGYHAAGKLKTRESVVEGLANAPHGLIALLKGGNFGKQLVKLA"
     gene            2616..3050
                     /locus_tag="VIG70_16675"
     CDS             2616..3050
                     /locus_tag="VIG70_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY8252234.1"
                     /translation="MASSWSSSPDYAGFWARAAAFLIDWLIAIVIAVPVMVVGFGLRE
                     VSLDPAEHSWDLFGFLAIAAVVIGFWRYCGATPGKLALAVRIVDAATGEPPSTARLVL
                     RFFAYFVSALPLYLGFLWIAVDRRKQGWHDKIARTIVINSEE"
     gene            3053..3745
                     /locus_tag="VIG70_16680"
     CDS             3053..3745
                     /locus_tag="VIG70_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY8252235.1"
                     /translation="MIDLYTWPTPNGHKVHIMLEETGTPYRVIPVDIGVGDQFKPDFL
                     KISPNNKMPAMVDPDGPDGKPFALAESGAMLFYLASKTGRFLPKNLRKRWQVMQWTMF
                     QMGHIGPMLGQAHHFLGYAPEKIEYAMNRYKNEANRLYGVLEKRLRESKYVACDEYTI
                     ADMATMPWLRAPERQGVNIDDYPALKRWRDAILERPAVQRAVQVLADRRRTQMTDQQR
                     ENLFGAQQYAKR"
     gene            3720..4487
                     /locus_tag="VIG70_16685"
     CDS             3720..4487
                     /locus_tag="VIG70_16685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8252236.1"
                     /translation="MPSNTLNGKVAWITGGGSGIGLAGGLELARAGAHVVISGRSAET
                     LKRAEKELKAAGSCETIALDVADQKEVARAAAEITKRHRRVDILVNSAGLNHPKRRFR
                     DVSIDGWNQIVAVNLSGTFYCCQAVLPGMRERKDGMIINVSSWFGRYWNTLGGPGYNS
                     TKHAVVSLTETLNVEEAANGIRATCILPGEVATPILEKRPVPPPPHERARMLQAEDLG
                     RAILFVATLPRRACVNELIISPTWNRFYLGGLETPKN"
     gene            4412..5707
                     /locus_tag="VIG70_16690"
     CDS             4412..5707
                     /locus_tag="VIG70_16690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8252237.1"
                     /translation="MRQRAHHQPDLEPVLPRRARNAEELNRLFFRVSAPFLAGYFVSY
                     VYRMVNAVLAPTLAAEFGLSAGGLGLLSSMYFFSFALCQLPVGLAMDRFGPRRVNAAL
                     LLVAAAGGAWFASADTAAGAMAARALVGAGVSVALMSSLTAFVLWYPADRLATMNAVA
                     FSVGMVGAMAVTVPLELLLRVWPWRDAFLLIVALTVAVSLVLWLWVPERRAQPRGKPF
                     MEEIHELRQLIADPAFLRLAVCLGASQFAAISLQTLWIATWLRDVAGYTPAEVARGLL
                     AVNAAMIVGYLAFGRAADLLQRRGRSALPLLAGGVAVSSLSLGLLALGVKMLFLWCIF
                     VAAATAVVLGYAIVSRRYPRAMAGRANTAINVFAFVGMFTGQWGIGLVLDLWPQSARG
                     YAAEAYPWALGMTLAVQLAGLAWLWSGRTLLESRSTGAA"
     gene            complement(5691..6473)
                     /locus_tag="VIG70_16695"
     CDS             complement(5691..6473)
                     /locus_tag="VIG70_16695"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEY8252238.1"
                     /translation="MTTNPYAAPKAAVADEAIVLNADFVPGGQSRPAGNGWTWIAEGW
                     ELFKRQPGLWIGMVLLAAVIFIAASMIPIVGPLATTLFGPVFAAGVMVGCKALDSGGE
                     LELGHLFAGFRERTGTLIAVGALYLAATVVVIVVVSLVMGVGMMTMMGAGDPQEVAAM
                     GMTILLAVLIMFALLLPAVMAIWLAAPLVVFHEHGAVEAMKGSFAGCLKNLLPFLVYS
                     VVLFVFAIAATLPLMLGWLVLGPVFAASVYASYRDIYLKLRQ"
     gene            complement(6470..6889)
                     /locus_tag="VIG70_16700"
     CDS             complement(6470..6889)
                     /locus_tag="VIG70_16700"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY8252239.1"
                     /translation="MKLEFAREYQKKIPYLQHLKIQTDELGEGTARLSLPIEPQLTNS
                     LGTAHGGVIMSLLDVALCTAARTLHPDSIGVITIDLSVSFIGGASGERLAAEARVLKD
                     GRSMSFVEAEAKNQDGSLVAKAIATVRVRHREKKGET"
     gene            complement(6886..7653)
                     /locus_tag="VIG70_16705"
     CDS             complement(6886..7653)
                     /locus_tag="VIG70_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8252240.1"
                     /translation="MGVKQLLDLSGRVALVTGGSRGLGLQIAEALAEMGAKIAISARK
                     KDELDQAVSRLGGEASAFVCDIGKREAIAPLADAVLKKFGRVDILVNNAGATWGAPAE
                     THPLEAWDKLVNVNLSGAFVLTQIVAAKSMIPARWGRIINVASIAGLIGQDPRFAPTA
                     AYTATKHGLVGLTRQLAAEWGVHGITVNAICPGFFPSKMTKATLETTGAQIREWTPNR
                     RLGNDEDLKGLAVLLASEAGRHITGQAIAVDGGATIV"
     gene            7788..8402
                     /locus_tag="VIG70_16710"
     CDS             7788..8402
                     /locus_tag="VIG70_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252241.1"
                     /translation="MTTLVMFSGGLDSTAMLVKLLAERADDLRVHHIRMVNREGRDRA
                     EQRAVEAIVAYCRARYRAFRYSESALDFSALEAIPIDYLSIAFVACQVAIDTPGCDRI
                     AIGALATDTDIANRSARQKRVFDEMYACYRTRKLGEPRVEWTFPVYDTPKAELAAGLP
                     QELLDLTWSCRRPLEGFRPCLTCKACLARQGLRRETAPAQNRVL"
     gene            8416..9648
                     /locus_tag="VIG70_16715"
     CDS             8416..9648
                     /locus_tag="VIG70_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252242.1"
                     /translation="MRSRRNDGRILIYSHDTFGLGHLRRCRAIAHSLVESFKQLSVLI
                     LSGSPIIGSFDFRARVDFVRVPGVIKLRNGDYTPLNLHLDIRETMSMRASIIQHTAEH
                     FEPDMFIVDKEPVGLRGELTQTLKMLKRRGTRIVLGLRDVMDEPRLLEPEWQRKKAVP
                     ALREFYDEIWVYGLPQICDPLEGIALPSRVREKMVYTGYLHREVPTHGAPPRLPEIAN
                     RPFLLVTTGGGGDGEGLIEWVLRAYEHDPLLPYPALLVLGPFMQAERQAEFMDRAAKL
                     KRVDAITFHGQIEALVARAAGVVAMGGYNTFCEVLSLDKRALIVPRTEPRLEQLIRAS
                     RAAQIGLVSMLSDDGTYDPAVMAAALRALPRQNLPSEVVVPGLLEGLKNVSRLVAPWI
                     EHAEAEVRPVSRIMSRIG"
     gene            9661..10914
                     /locus_tag="VIG70_16720"
     CDS             9661..10914
                     /locus_tag="VIG70_16720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8252243.1"
                     /translation="MRVAFILKGYPRLSETFIAQEIAALERRGLEIHIVSLRRPTDSR
                     RHPVHGEIRAAVLYLPEYLILEPLRVLRSWWQFRKLEPYRKARSLWIKDLLRDPTPNR
                     VRRFGQALVLAAELPAGVRHLHAHFLHTPASVTRYAATLLGLPWTGSAHAKDIWTLPE
                     WEKREKLASCQWLVTCTRANRDHLAALAPPGRVALVYHGIDLTRFSPKAFTKPAIRDG
                     TSAADPVVILSVARLVEKKGTEVLLEALARISPRLHWRFVHAGGGPLKRRLARLAVEL
                     RISDRVEWRGAMTQDRLLAEYRNADLFALASRVARDGDRDGLPNVLAEAQSQALACVA
                     TRVSAIPELVRDGQTGLLVEENDPDALARALEALITNPARRRSLGQAGQSRVVEAFAL
                     DASLEPLARKFGLGASGANRVLRTA"
     gene            10886..12013
                     /locus_tag="VIG70_16725"
     CDS             10886..12013
                     /locus_tag="VIG70_16725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8252244.1"
                     /translation="MRIAFYAPLKPPTHATPSGDRRVAGLLMDALERVADHIELVSTF
                     RSFDGDGDGARQVALREQGAALAQRLADGWRAGPAALRPDLWFTYHVYYKAPDWLGPA
                     VSAALGIPYVIAEASYAEKRAGGPWAIGHEAAADAINRARLVISPSRDDLAGLKEVVA
                     PERLVHLPPFLDPAPYRDAAKERAAHRARLARAHGLDPAVPWISVVAMMRPGDKLASY
                     HSLAATLGGLTDLSWRLVVVGDGSARQEVRAAFEQAAPGRACFLGGLENREVAAISAA
                     CDLYAWPAVNEAYGMAMLEAQAAGLPVVSVAKRGVPDVVIHGRTGLLAADEKTLGEPL
                     RELLVDGVRRAALGRAAAAFIQRERSLEAAAVQLKSLLGGL"
     gene            12010..12573
                     /locus_tag="VIG70_16730"
     CDS             12010..12573
                     /locus_tag="VIG70_16730"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8252245.1"
                     /translation="MTALALLRHADTAWSVEGRIQGRRDVPLLAGSSCSLPDACRGMR
                     VVTSPLARCVQTAALIGAAGATKEPRIIEMDWGAWEGESLAALRERLGEEMRDNEARG
                     LDFMPPGGESPRRVTERLKPWLQELARAGMPTLAVTHRGVIRAILAEATGWDMRARPP
                     AKLDWEAVHFFLLDRQGTPSVQRLNVR"
     gene            12570..13667
                     /locus_tag="VIG70_16735"
     CDS             12570..13667
                     /locus_tag="VIG70_16735"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8252246.1"
                     /translation="MRVFFYVQHLLGIGHLKRAATLAGALRRAGFDVTLASGGAPVDG
                     IPVLQLPSASSPDFKQLLDEDGNAVDDAWKTRRRDALLDAFQRTSPGVLLIELFPFGR
                     RQMRFELLPLLESAHRQDPKPLVVCSVRDLIQPNPARENERLELFDRYFDHLLVHGDP
                     AIAIFERSFGRAAGLAGKLHYTGYVVDEVPLRSEVGKDEVLVSAGGGAVGRHLLETAI
                     QARPLTGLRDRTWRILAGSNSAQFERLASQASQDVIVERARNDFTLRLRNCVLSVSQA
                     GYNTVLETLQAGAKAVLVPFAGGGESEQTLRARLLAERGLVHVVEEDVLTAATLAAAV
                     DRAAAGPRPAPGAVDLGGARRSAELLRQWVT"
     gene            13664..14401
                     /locus_tag="VIG70_16740"
     CDS             13664..14401
                     /locus_tag="VIG70_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY8252247.1"
                     /translation="MTRACVNWEEFEDELARRRKAGRPVQFWWRDDDAAAVLPSLKQL
                     LSISKKRQTPLALAVIPEAAEPELFRVLHDGVTVLQHGTDHRNRAAAGEKKTEYPAGE
                     AVEAALARISDGLGRLRSLAGNRFLPVLAPPWNRVRRDILKKLPAIGVRGVSAYGARA
                     GAEPVPGLRQVNTHVDIIAWQEGKRFIGEAEALSKAVSYLSGDEPLGWLTHHAVHDAA
                     AWNFLERLFTLEDVRWLSAAEAFSPNL"
     gene            14438..>14602
                     /locus_tag="VIG70_16745"
     CDS             14438..>14602
                     /locus_tag="VIG70_16745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8252248.1"
                     /translation="MADLLTVRDLKVYLDVDAGTVKAVDGVSFRIPAGGTVALVGESG
                     SGKSVVAQSIM"
BASE COUNT         2289 a         4892 c         5003 g         2418 t
ORIGIN      
        1 cccgtgagct cgagcggcgc tacctcgaca tgcgcaagac cacgatctac gccggctcca
       61 acgagatcca aaggaacatc atcgccaaga tgacgttggg tctatgaact tcgactacaa
      121 cgaagagcag cagcttctcg cggattccgt ccggcggttc ctgcagaagg actacgactt
      181 cgaggcacga aagcaaatcg tcgcctcgcc ggcgggctgg agcgagcgag tgtgggcgac
      241 cttcgccgag atgggcctca ccggcctgcc gatctcgccg gactatggcg gcttcggcgg
      301 cggcgctgtc gacctgatga gcgtgatgga agctttcggc gaagcgctgg tggtggaacc
      361 ctatctgccg accctcttgg cggcggctgc gatcgccaag gcgggggatg aaaagcagaa
      421 gcaagccgtc ttgccgctcg tcgcgcaagg caagctgcgg ctcgcgctgg cgaccggtga
      481 gcgcggcgtc attggcgccc cggtcgcaga caggatcgtg ttcttccggc aagggaagct
      541 ccaggctgtg gagcgcagct cgctgcagct caaggcctat cggacgctcg acgacttggc
      601 cgccgccgac atcgccttcc aggccgagag ctcgaacccg gtgctcgacg aggtgctcga
      661 tttcggctcg gcgcttgcct gcgccgaggc ggtcggcgcg atgaagttcg cgtgcgacac
      721 cacgctcgag tacctgaaga cgcgccgcca gttcggcgtg ccgatcggtg cgttccaggc
      781 gctgcagcac cgcatggtcg acatgttcat ttcctacgag caggcgaaat ccatggcttg
      841 ccttgcctgc tcgaaggtcg accagtcgga aagcgcagcc gagcgcgcgc gcgccgtttc
      901 ggccgccagg atcaagatcg ccgacagcgc gcgccacatc agccaggaat cggtgcagct
      961 ccacggcggc atggggatga gcgaggagct gaaagtgagc cacaccttcc gccgcctgac
     1021 cgtgctccag cggcgcttcg gcgacgtcga tcgacacctc gagcgctttg cctcgcttta
     1081 gagcttgggt ggtcatcgtg ctcgtcggct gcgcacagca gcccgcgaag caggacgaaa
     1141 ggaaagcgcc gccggaagcg ctgccgccgt tgccggccac gtacgtcaac gactccgact
     1201 gccccggctg tctcgaggtg acgctcacgc tgcgccccga cggcagctac ctcgtgcgcg
     1261 accggctcgg cgcttccgag ttctacgatt tcgggcgctg gaagtattcc gacggtacgc
     1321 tcgagctcgc cggcgggcgc gacgcgccgc gccgctaccc gttgaaagag ctcaggaaaa
     1381 ctccgcaagt ggaggcgctg cgcggtccct tccgcatggt cgggctctac gacggggccg
     1441 ggttcaagga atgccgcacc gggctgacct ggcagcttgc ggacacgcgc gcagcggaaa
     1501 acctgaaggg cgattttctg aaagggcagg ggaacgcggt cctcgccgtg atcgacgccc
     1561 agttcgaggg cgcgcccgag atgttgcgcg tattgcgccc ggcttcggtg ctcgatgcgc
     1621 gcacctgtcc cgctcacttg tccggctaaa cttcgacgcc atggaaaacc aacgcatcgt
     1681 gctcgccagc cgtcccgagg gatgggtcac cctggacaat ttccgcctgg agaaaggccc
     1741 gctgccgaaa atcggggacg gcgaggtcct ggtgaagaac ctctggctct cgctcgaccc
     1801 gtacatgcgc ggacgcatga gcgacaccaa gagctacgtg aagggcgtgg acctcggcga
     1861 ggtcatggtc gggcagaccg cgggcgaggt cctcgaatcg aaaaaccctg cgttcaagaa
     1921 aggcgaccag gtcctgactc agctcggctg gcagctttat ggggttacga aggaagtcat
     1981 gaaagtggat gggtcgcgag cgccgctcag ctactacctc ggggtgctcg gaatgccggg
     2041 catgaccgcg tacttcggcc tgaaggagat cggccagcca aaggccggcc agaccgtggt
     2101 ggtgtcggcg gcttccggcg ccgtgggcag cgtggtcggg caattggcca agctcttggg
     2161 ctgccgtgcg gtcggcatcg cgggcggccg cgagaagtgc gactacgtga cgcgcgagct
     2221 cggcttcgac gcttgcatcg actacaaggc cggcaagctg cgcgacgagc tgaaggaact
     2281 caagggaatc gatgtctatt tcgacaacgt gggcggcgag atcctggacc tggtcctcac
     2341 gcgcatgaat ctcttcggcc gcatcgtcgt gtgcgggatg atcgccgact acaacgcgac
     2401 cgagccgtat cgcttcagga acctgcgcgc tgtgctggtg aaccggctga agatccaggg
     2461 gatgatcgtg ttcgactgga aggagcgcta cggcgaggcc ctgaaagcgc tcggcggcta
     2521 tcacgcggca gggaagctca aaacgcgcga gtcggtggtc gaggggctgg cgaacgcgcc
     2581 gcacggcctg atcgcgctgc tgaaaggcgg aaattttggc aagcagctgg tcaagctcgc
     2641 ctgactacgc gggcttctgg gcgcgcgcgg cggcgttcct gatcgactgg ctgatcgcga
     2701 tcgtgatcgc ggtgccggtc atggtggtgg gattcggcct gcgcgaggtc tcgctcgacc
     2761 cggccgagca ttcctgggat ctgttcggct tcctcgcgat cgcggcggtg gtaatcggtt
     2821 tctggcgcta ctgcggcgcg accccgggca agctcgcgct cgcggtgaga atcgtcgatg
     2881 ccgctaccgg cgagccgccg agcaccgcgc ggctcgtgct gcgcttcttc gcgtacttcg
     2941 tttccgcgct accgctgtat ctcggcttcc tgtggatcgc ggtcgaccgc cgcaagcagg
     3001 gctggcacga taagatcgcc cgcaccatcg tgatcaattc agaggagtag gtatgatcga
     3061 tttgtacacg tggccgacac ccaacggcca taaggtccac atcatgctcg aggagaccgg
     3121 cacgccgtac cgcgtgatcc cggtcgacat cggcgtgggc gaccagttta agccggactt
     3181 cctcaagatt tcccccaaca acaagatgcc ggcgatggtc gatcccgacg gcccggacgg
     3241 caagccgttc gcgctcgcgg aatcgggcgc gatgctgttc tacctcgcgt cgaaaaccgg
     3301 caggttcctg cccaagaact tgcgcaagcg ctggcaggtg atgcagtgga ccatgttcca
     3361 gatgggccac atcggcccga tgctcggcca ggcgcaccac ttcctcggct atgcgccgga
     3421 gaagatcgag tacgcgatga accgctacaa gaacgaagcg aatcgtcttt acggggttct
     3481 cgaaaaaaga ttacgcgaaa gcaagtacgt ggcgtgcgac gagtacacga ttgccgacat
     3541 ggcgaccatg ccgtggctgc gcgcgcccga gcgccagggc gtgaacatcg acgactatcc
     3601 ggcgctcaag cgttggcgcg acgcgatcct cgagcgcccg gcggtgcagc gcgcagtgca
     3661 ggtgctggcc gaccggcggc gcacgcagat gaccgaccag cagagagaga acctgttcgg
     3721 tgcccagcaa tacgctaaac ggtaaggtcg cctggatcac cggcggcggc tcggggatcg
     3781 ggctcgccgg cggcctcgag ctcgcgcgag ccggcgcgca tgtcgtgatc tcgggccgca
     3841 gcgccgaaac gctgaagaga gccgagaagg agctcaaggc ggccgggagc tgcgagacca
     3901 tcgcactcga cgtcgccgac cagaaggagg tggcgcgcgc cgccgccgag atcacgaagc
     3961 gccaccgccg cgtcgacatc ctggtgaaca gcgccggcct caaccatccg aagcggcgct
     4021 tccgcgacgt gtcgatcgac ggctggaacc agatcgtggc ggtcaacctc tcggggacct
     4081 tctactgctg ccaggcggtg ctgccgggca tgcgcgagcg caaggacggc atgatcatca
     4141 acgtctcgtc gtggttcggg cgctactgga acacgctggg cggccccggc tacaactcca
     4201 ccaagcacgc ggtggtctcg ctcacggaga cgctcaatgt ggaggaggcg gccaacggaa
     4261 tccgcgccac ctgcatcctg ccgggcgagg tggctacgcc catcctggaa aagcgcccgg
     4321 tgccgccgcc gccgcacgag cgcgcgcgca tgctgcaggc ggaggacctc ggccgggcga
     4381 tcctgttcgt cgccacgctg ccgcggcgcg cgtgcgtcaa cgagctcatc atcagcccga
     4441 cctggaaccg gttctacctc ggcgggctcg aaacgccgaa gaactgaacc gccttttttt
     4501 ccgcgtctcg gccccgtttc tcgcggggta tttcgtgtcg tacgtgtacc gcatggtcaa
     4561 cgcggtgctc gccccgacgc tcgccgccga gttcggcctg tcggccggcg ggctgggact
     4621 cttgtccagc atgtactttt tctccttcgc gctttgccag ctccccgtcg gactggcgat
     4681 ggaccgcttc ggcccgcgca gggtcaatgc cgcgctgctc ctggtcgccg ccgcgggcgg
     4741 cgcctggttc gccagtgccg acacggcggc gggcgcgatg gcggcgcgcg cgctggtggg
     4801 cgccggcgtc tcggtggcgc tcatgtcctc gctgaccgcg ttcgtgctct ggtatccggc
     4861 cgaccggctc gcgaccatga acgcggttgc cttctcggtc ggcatggtgg gcgccatggc
     4921 ggtcacggtg ccgctcgagc tgctgctgcg cgtctggccc tggcgcgatg ccttcctgct
     4981 gatcgtcgcc ctcaccgtgg cggtcagcct ggtgctttgg ctctgggttc cagagcgccg
     5041 cgcgcagcct cgcggaaagc ctttcatgga ggaaatccat gagctgcgac aactgatcgc
     5101 cgaccccgcc ttcctgcgcc tcgcggtttg cctcggcgcg agccagttcg ccgccatctc
     5161 gctgcagacg ctgtggatcg ccacctggct gcgcgacgtg gcgggctaca cgcccgccga
     5221 ggtggcgcgc ggcctgctcg cggtgaacgc ggcgatgatc gtcggctatc ttgcgttcgg
     5281 ccgcgccgcc gaccttctgc agcgtcgcgg gcgcagcgcg ctgccgttgc tggcgggcgg
     5341 cgtggcggtg tcctcgctca gcctcgggct gctcgcgctc ggcgtgaaaa tgctttttct
     5401 ctggtgcatc ttcgtcgccg cggctacggc ggtcgtgctg ggctacgcga tcgtgtcgcg
     5461 ccgctacccg agggcgatgg ccgggcgggc gaacaccgcg atcaacgtgt tcgctttcgt
     5521 gggcatgttc accgggcagt ggggaatcgg cctggtgctg gatctgtggc cgcagagcgc
     5581 acgaggctac gccgccgagg cgtatccgtg ggcgctcggc atgaccttgg cggtgcagct
     5641 cgccggcctc gcctggctgt ggagcggccg gactcttctg gagagccgct ctactggcgc
     5701 agcttgaggt agatgtcgcg gtacgaggcg tagaccgacg ccgcgaacac cggcccgagc
     5761 accagccagc ccagcatcaa gggcagggtg gcggcgatcg cgaacacgaa gagcaccacg
     5821 ctgtagacca ggaagggcag gaggttcttc aggcaccccg cgaagctgcc cttcatcgcc
     5881 tcgaccgcgc cgtgctcgtg gaatacgacg agcggcgcgg caagccagat cgccatcacc
     5941 gccggcagca gcagcgcgaa catgatcagc acggcgagca agatggtcat ccccattgcc
     6001 gccacctcct gcgggtcgcc ggcgcccatc atggtcatca tgccgactcc catgaccagg
     6061 ctgacgacca cgatgacgac caccgtcgcc gcgagataga gcgcgcccac cgcgatcagc
     6121 gtgccggtgc gctcgcggaa gccggcgaag agatggccga gctcgagctc gccgccgctg
     6181 tcgagcgcct tgcagccgac catcacgccg gccgcgaaca ccggcccgaa cagcgtcgtg
     6241 gcgagcgggc cgacgatggg aatcatcgag gcggcgatga agatcacggc agcgagcagg
     6301 accatgccga tccacaggcc gggctggcgc ttgaagagct cccagccctc ggcgatccag
     6361 gtccagccgt tgcccgcggg gcgcgactgg ccgcccggaa cgaagtccgc gttcaacacg
     6421 atcgcctcat ccgcgaccgc ggctttcggc gctgcgtaag gattcgtcgt catgtctctc
     6481 ccttcttctc cctgtgtcgc acgcggaccg tggcaatcgc tttcgccacc agcgatccgt
     6541 cctggttctt cgcttccgcc tcgacgaagc tcatgctgcg tccgtccttg agcacgcgcg
     6601 cttcggcagc gaggcgctcg ccgctcgcgc cgccgatgaa cgacacgctg aggtcgatgg
     6661 tgatcacgcc gatcgagtcc gggtgcagcg tgcgcgccgc cgtgcacagc gccacgtcga
     6721 gcagcgacat gatgacaccg ccgtgcgcgg tgccgaggct gttggtgagc tgcggctcga
     6781 tcggaagcga caggcgcgcc gtgccttcgc cgagctcgtc ggtctggatc ttcagatgct
     6841 ggaggtacgg gatcttcttc tggtattccc gggcgaactc gagcttcata cgatggtggc
     6901 gccgccgtcg accgcgatcg cctgcccggt gatgtgccgc cccgcctcgg aggcgagcag
     6961 cacggcgagg cctttcaggt cctcgtcgtt gccgaggcgc cggttcggcg tccattcgcg
     7021 gatctgcgca ccggtcgtct cgagggtcgc cttggtcatt ttcgagggaa agaacccggg
     7081 gcagatcgcg ttcaccgtga tgccgtgcac gccccactcc gcagcgagct ggcgcgtcag
     7141 gccgacgagg ccgtgcttgg tcgccgtgta ggcggcggtg ggcgcgaagc gcgggtcctg
     7201 gccgatcagc cctgcgatcg aggcgacgtt gatgatgcgc ccccatcttg ccgggatcat
     7261 cgatttggct gcgactatct gcgtcagcac gaaagcgccg ctcaagttca cgttgacgag
     7321 cttgtcccag gcctcgagcg gatgcgtctc ggccggcgcg ccccacgtgg cgccggcgtt
     7381 gttcaccagg atgtcgacgc gcccgaactt cttcaggacc gcgtcggcga gcggcgcgat
     7441 cgcctcgcgc ttgccgatgt cgcaaacgaa tgcgctggct tcgccaccga ggcgcgacac
     7501 tgcctggtca agctcgtctt tcttgcgagc gctgatcgcg atcttcgcgc ccatctctgc
     7561 cagcgcctct gcaatctgca ggccgagccc gcgcgagccg ccggtgacca gcgcgactct
     7621 tccacttagg tccagcagct gtttgacgcc cacaaggccg aatcctagta caagggcgaa
     7681 aatcagggac agaccacgtt ttccagccaa tgggcgcagc cgaaaacgtg gtctgtccct
     7741 gattctggtc tgctgattcc ggtctgtacg acatctacaa tgcggatatg accacgctgg
     7801 tgatgttttc gggtgggctc gacagcacgg cgatgctggt gaagctgctc gccgaaaggg
     7861 ctgacgacct gcgcgtgcat cacattcgca tggtgaaccg ggaaggccgg gaccgcgccg
     7921 agcagcgcgc ggtcgaggcg atcgtggcgt actgccgcgc gcgttaccgg gcgttccgtt
     7981 actccgaatc ggcgctcgac ttcagcgcgc tggaggcgat cccgatcgac tatctgtcga
     8041 tcgcgttcgt cgcctgccag gtcgcgatcg atacaccggg ctgcgatcgc atcgcgatcg
     8101 gcgcgctcgc cacggacacc gacattgcga accgctcggc gcgccagaag cgcgttttcg
     8161 acgagatgta cgcgtgctac cgcacgcgca agctcggcga gccgcgggtc gagtggacct
     8221 tcccggtcta cgacaccccc aaggccgagc ttgcggcggg gctgccgcaa gagctgcttg
     8281 acctgacatg gtcctgtcgt cgcccgctgg agggctttcg cccttgcctg acttgcaagg
     8341 cttgcctcgc ccggcagggc ttgcgccggg agaccgcccc ggcccaaaat cgggtcttat
     8401 aaggggaggg gcgccatgag atccagacgg aacgatggcc gcattctcat ttacagccac
     8461 gacaccttcg gcctcggcca cctgcgccgc tgccgggcga tcgcgcattc gctcgtcgag
     8521 agcttcaagc agctctcggt cctgatcctc tcgggctcgc cgatcatcgg cagcttcgac
     8581 ttccgcgcgc gcgtcgactt cgtgcgcgtg ccgggggtga tcaagttgcg caacggcgat
     8641 tacacgccgc tcaacctgca cctcgacatc cgcgagacca tgtcgatgcg cgcctcgatc
     8701 atccagcaca ccgccgagca tttcgagccc gacatgttca tcgtcgacaa ggagccggtc
     8761 gggctgcgcg gcgagctcac gcagacgctg aagatgctca agcgccgcgg cacgcgcatc
     8821 gttctgggcc tgcgcgacgt gatggacgag ccgcgcctcc tcgagcccga atggcagcgc
     8881 aagaaagcgg tgccggcgct gcgcgagttc tacgacgaga tctgggtcta cgggctgccg
     8941 cagatctgcg acccgctcga aggcatcgcg ctgccctcgc gcgtgcgcga gaagatggtc
     9001 tacaccggct acctgcaccg cgaggtgccg acgcacggcg ctccgccgcg cctgcccgag
     9061 atcgccaacc ggccgttcct gttggtgacc acaggcggcg gcggcgacgg cgaggggctg
     9121 atcgagtggg tgctgcgcgc ctacgagcac gatccgctgc tcccctatcc ggcgctgctc
     9181 gtgctcgggc ccttcatgca ggccgagcgc caggccgagt tcatggaccg cgcggcgaag
     9241 ctgaaacgcg tcgacgcgat caccttccac ggccagatcg aggcgctggt ggcgcgcgcg
     9301 gccggggtgg tggcgatggg cggctacaac accttctgcg aggtgctgtc gctcgacaag
     9361 cgcgcgctga tagtgccgcg caccgagccg cgcctcgagc agctcatccg cgcctcgcgc
     9421 gcggcccaga tcgggctcgt gagcatgctg agcgacgacg ggacctacga tccggcggtg
     9481 atggccgcgg cgctgcgcgc cctgccgcgc cagaacctgc cctcggaagt cgtggtgccg
     9541 gggctgctcg aaggcctgaa gaacgtctcg cggctcgtcg cgccgtggat cgagcacgcc
     9601 gaagccgagg tgcggccggt ttcgcgcatc atgtcgcgca tcggctagcg cgggtcgctg
     9661 gtgcgggttg ccttcatact gaaggggtat ccgcgcctct ccgagacgtt catcgcgcag
     9721 gagatcgcgg cgctcgagcg tcgcggcctc gagattcaca tcgtttcgct gcgccggccc
     9781 accgactcgc gccggcaccc ggtgcacggc gagatccgcg ccgcggttct ctacctgccc
     9841 gagtacctga tcctggagcc gttgcgtgtg ctgcggagct ggtggcagtt tcgaaagctc
     9901 gaaccctacc ggaaagcccg cagtctctgg atcaaggacc tgctgcgcga tcccacgccg
     9961 aaccgcgtgc gccgcttcgg gcaggcgctg gtgctcgcgg ccgagcttcc ggccggcgtc
    10021 aggcatctgc acgcgcactt cctgcatacg cccgcttcgg tgacgcgcta cgccgcgacg
    10081 ctcctcggcc tgccgtggac gggctcggcg catgccaagg acatctggac cctgcccgaa
    10141 tgggagaagc gcgagaagct cgcgtcctgc cagtggctgg tcacctgcac gcgggcgaat
    10201 cgcgaccacc tcgccgcgct cgcgccgccg gggcgcgtgg cgctggttta ccacggcatc
    10261 gatctcacga ggttttcacc aaaagctttt acgaaaccag ccatacgcga tggcacttct
    10321 gccgccgacc cggtggtgat tctttcggtg gcgcggctgg tggaaaagaa gggcaccgaa
    10381 gtgctgctcg aggcgctggc gcgcatttcg ccgcggctgc actggcgatt cgtccacgcg
    10441 ggcggcggtc cgctcaagcg gcgcctcgcg cgacttgctg ttgaattgag aatttcagac
    10501 cgcgtggaat ggcgcggcgc aatgacgcag gatcggctgc tcgctgaata ccggaatgca
    10561 gacctgttcg ccctcgcgag ccgcgtggcg cgcgacggcg accgcgacgg gctgcccaac
    10621 gtgctcgccg aggcgcagag ccaggcgctc gcctgcgtgg cgacccgcgt gtcggcgatc
    10681 ccggagctcg tgcgcgacgg ccagaccggg ctgctggtcg aggaaaacga ccccgacgcg
    10741 ctggcgcgcg cgctcgaagc gctgatcacg aatccggcgc ggcgccgctc gctcggccag
    10801 gccgggcagt cgcgcgtggt cgaagccttc gcgctcgatg cgagcctcga gccgctcgcg
    10861 cgcaagttcg ggctcggcgc gtccggtgcg aatcgcgttc tacgcaccgc ttaagccgcc
    10921 gacgcacgca acgccttcgg gcgatcggcg cgtcgccggg ctgctgatgg acgcgctcga
    10981 gcgcgtcgcc gaccacatcg agctggtgtc caccttccgc agcttcgacg gcgacggcga
    11041 cggcgcgcgc caggtagcgc tgcgcgagca gggcgcggcg ctggcgcaac gcctcgccga
    11101 cggatggcgc gccgggcccg ccgcgctgcg gcccgatctc tggttcacct atcacgtcta
    11161 ctacaaggcg ccggactggc tcggaccggc ggtgagcgcc gcgctcggca ttccgtacgt
    11221 cattgccgaa gcctcctacg cggagaagcg cgccggcggc ccctgggcga tcggccacga
    11281 ggcggcggcg gacgcgatca accgcgcgcg cctggtgatt tccccgtcgc gcgacgatct
    11341 cgccggcctg aaggaggtcg tcgcgccgga gcgcctggtg cacctgccgc cctttctcga
    11401 cccggctccg taccgcgacg cggcgaaaga gcgcgcagcg catcgcgcac ggcttgcgcg
    11461 cgcgcacggc cttgaccccg ccgtgccgtg gatctcggtc gtggcgatga tgcgcccggg
    11521 cgacaagctc gcttcgtacc actcgctcgc cgccacgctt ggcggcctca ccgatctttc
    11581 ctggcggctc gtcgtcgtcg gcgacggctc ggcgcggcag gaagtgcgcg ccgccttcga
    11641 gcaggcggcg ccggggcgcg cctgctttct gggaggactg gagaaccggg aagtcgcggc
    11701 gatctccgcg gcttgcgacc tctacgcctg gccggcggtc aacgaggctt acggcatggc
    11761 gatgctcgaa gcgcaggcgg ccggccttcc cgtggtgtcg gtggccaagc gcggagtgcc
    11821 cgacgtcgtg atccacggcc gcaccgggct tctcgctgcc gacgagaaga ctctcggcga
    11881 gccgctgcgc gagcttctcg tcgatggcgt tcgccgcgcc gcgctcgggc gagccgcggc
    11941 ggcgttcatc cagcgcgaaa gaagcctcga ggccgcggcc gtgcagctga agagcctcct
    12001 cggcggactg tgaccgcgct tgcgctgctg cgccacgcag acaccgcgtg gagcgtagag
    12061 gggcgcatcc aggggcgccg cgacgtgcct ttgcttgccg ggagctcctg ttctctgccc
    12121 gacgcgtgcc gcggcatgcg cgtggtcacc agccctctcg ctcgctgcgt ccagaccgcg
    12181 gcgctgatcg gcgcagccgg ggcgacaaag gagccgagga tcatcgagat ggactggggc
    12241 gcctgggaag gcgaatcgct cgcggcgctg cgcgagcgcc tcggcgagga gatgcgcgac
    12301 aacgaagcgc gcggcctcga cttcatgccg ccgggaggcg aaagcccgcg gcgggtgacc
    12361 gaaaggctga agccctggct gcaggagctc gcgcgcgccg gcatgcccac gctggcggtc
    12421 actcatcgcg gcgtgatccg cgccatcctc gccgaggcaa ccggctggga catgcgcgcc
    12481 aggccgccgg cgaagctcga ctgggaggcc gtacacttct ttcttctcga ccggcaggga
    12541 acacccagcg tgcagcgcct gaatgtgcgg tgagagtttt cttctacgtt cagcacctgc
    12601 tcggcatcgg gcacctgaag cgcgcggcga cgctcgccgg cgcgctgcgc cgcgcgggat
    12661 tcgacgtcac gctcgcgagc ggcggcgcgc cggtagacgg gatccccgtt ctgcagttgc
    12721 cgagcgcatc gagccctgat ttcaagcagc tcctggatga agacggcaac gcggtggatg
    12781 acgcctggaa gacgcggcga cgcgatgcgc ttctcgacgc cttccagcgg acctctcccg
    12841 gcgtattgct gatcgagctc tttccgttcg gccggcggca gatgcgcttc gagttgctgc
    12901 ctctgctcga atctgcccat cgacaagatc ccaaacccct ggtcgtctgc tcggtgcgcg
    12961 acctgatcca gccgaacccg gcgcgcgaaa acgagaggct cgagctgttc gatcgctact
    13021 tcgaccacct gctggtgcac ggcgatccgg ccatagcgat tttcgagcgg agcttcggaa
    13081 gggcggcggg cctcgccggc aagctgcact acaccggcta tgttgttgac gaagtacccc
    13141 taagaagtga agtaggcaaa gacgaggtcc tggtctcggc gggcggcggc gcggtcgggc
    13201 ggcacctgct cgaaacggcg atccaggccc ggccgctgac cggcttgcgc gaccggacct
    13261 ggcggatcct cgcgggttcg aactctgccc agttcgagcg gctcgcttcc caggcaagtc
    13321 aagacgtgat cgtcgagcgc gcccggaacg atttcacgct gcgcctcaga aactgcgtgc
    13381 tttcggtgag ccaggccggg tacaacacgg ttctggagac gctgcaggcg ggcgccaagg
    13441 cggtgctggt gccctttgcc ggcggtggcg agtccgagca gacgctacgg gcgagattgc
    13501 ttgccgagag ggggctcgtg cacgtggtcg aggaggatgt cctgacagcg gcgacgctcg
    13561 ccgcggcggt cgatcgcgcc gcggcgggcc cgcggccggc tccgggggcg gtcgatctcg
    13621 gcggcgcgcg ccgcagcgcc gagctgcttc gccaatgggt cacttgaccc gcgcttgcgt
    13681 gaactgggag gagttcgagg acgagcttgc tcgccggcgc aaggccggtc ggccggtgca
    13741 gttctggtgg cgcgacgacg atgccgccgc ggtgctgcct tccttgaagc aactgctttc
    13801 gatttcgaag aagcgtcaaa ccccgctcgc gctcgcggtg attcccgaag ccgccgagcc
    13861 cgagctcttc agggtgctgc acgacggcgt gacggtgctg cagcacggca ccgaccatcg
    13921 caaccgcgcc gcagccggcg agaagaagac cgaatatccc gccggcgagg cggtcgaggc
    13981 cgcgctcgcg cggatctccg acggcctggg caggttgcgg tcccttgcgg gcaacagatt
    14041 tctcccggtg ctcgcgccgc cgtggaaccg cgtgcgtagg gatatcttga aaaagctccc
    14101 ggccatcggc gtgcgtggag tgagcgccta tggtgcgcgc gcaggcgccg agcccgtgcc
    14161 cggcctgcgg caggtgaaca cccatgtcga catcattgcc tggcaagagg gcaagcgctt
    14221 catcggcgaa gcggaagcct tgtcgaaagc agtttcgtac ctctcgggcg acgaaccgct
    14281 gggctggctc acgcaccacg cggtgcacga cgcagcggcg tggaatttcc tcgaacgact
    14341 tttcaccctc gaggacgtgc gctggctctc ggcagcagag gccttctcgc cgaatttatg
    14401 agcggcttct ccgcgtactt atatactgcc cccgcgcatg gctgacctgc ttacggtccg
    14461 ggatctgaag gtttatctcg atgtcgatgc cggcacggtg aaggccgtcg acggcgtgtc
    14521 gttccgcatc ccggccgggg gcacggtggc gctggtcggc gaatcgggct cgggcaagtc
    14581 ggtggtcgcg cagtcgatca tg
//
LOCUS       DATBSZ010000261         9189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1417030, whole genome shotgun sequence.
ACCESSION   DATBSZ010000261 DATBSZ010000000
VERSION     DATBSZ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9189
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417030"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VIG70_16750"
     CDS             complement(<1..300)
                     /locus_tag="VIG70_16750"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8252249.1"
                     /translation="MYTSSVIDAPADTVWAQVRDFNGLPKWTPFVAESRIENGMPADR
                     IGCVRNFRLRDGGVIREQLLTLSDYDYQCSYSILESPMGVENYVATLKLTPVTDGN"
     gene            complement(306..1871)
                     /locus_tag="VIG70_16755"
     CDS             complement(306..1871)
                     /locus_tag="VIG70_16755"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HEY8252250.1"
                     /translation="MTGSQFGQFILALIGVAIVVAIVVWLLNWLYLRSSKERGFVRTG
                     FGGQKVVVNGGAFVLPIIHEVIPVNMNTLRLEVSRGRERALITKDRMRVDVTSEFYVR
                     VKAEAEAIAAAAQTLGQRTMQPESLKELVEGKFVDALRTVAAEMTMEEMHEKRGEYVK
                     RVRAAAAADLLQNGLELETVSLTQLDQTAMEFFNPSNAFDAEGLTRLTETIETRKKKR
                     NDIEQDTMIQIRNKNLDTERQALDIDRDLEYARLAQERELEVKRAVQRADVARQKATS
                     EQEAERAQITAREAVERARIVSERAVEQERIERERGIQELEIGRRQTIELAEQQRAIA
                     VAEHSKAQSEAQAAADHARAKAVAEEEKVFTVREVELAQRKKLIDVSNAEAEADTSRI
                     RSLAAKIRHEVEAEGLTLMNKAANLLTPEARQSAMRRQLIDKLEGIIRESVKPMERIE
                     GIRVLHVEGLGGGQSGPGNGNGGGNIADSVVNSALRFRAQAPLVDQLLREIGVQGGDL
                     GGAANALLEGKKP"
     gene            complement(1868..2710)
                     /locus_tag="VIG70_16760"
     CDS             complement(1868..2710)
                     /locus_tag="VIG70_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HEY8252251.1"
                     /translation="MARIARQDILKKLHAQARRGEPIVGGGAGTGLSAKCEEAGGIDL
                     IVIYNSGRYRMAGRGSLAGLMAYGNANEIVLDMAREVLPVVKHTPVLAGVNGTDPFLL
                     VESFLKRLCDLGFSGIQNFPTVGLFDGTIRAQLEETGMGYGLEVEVIRQAREMDLLTT
                     PYVFSDQNARDMAKAGADIVVCHLGLTAGGAIGARTALGLDQCIPLVNAWAQAARGVN
                     PDILVLCHGGPVATPQDAQYILDHCRGCNGFYGASSMERLPTEVALTEATRKFKGMKR
                     GVAS"
     gene            complement(2680..3891)
                     /locus_tag="VIG70_16765"
     CDS             complement(2680..3891)
                     /locus_tag="VIG70_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HEY8252252.1"
                     /translation="MAGTFDTKGRELFYLKSCLEKLGLRTVTVDLATSGKPSSANIHP
                     REVARHHPKGERAVFTGDRGSAVSEMGVAFENFVALRRDLGGLISAGGSGGTALATQA
                     MRRLPIGIPKVMVSTVASGDVKPYVGPSDICMMYSVTDISGINRISGKVLANAAHALA
                     GMIAYAKKEQEQTKPALGLTMFGLTTPCVQAVCKQLEARFDCIVFHATGTGGQSMEKL
                     VDSGLIAGVLDVTTTEIADELVGGVLTAGPTRMDVFARKQIPYVGSCGALDMVNFWAM
                     DTVPQQFKQRNLYRHNNNVTLMRTTPEENARIGRFIAEKLNRMQGPVRFLIPDGGVSG
                     IDTPGKPFWDPAADKTLFEAIHSNFRSTANRKLVRLPHHINDAAFAEALVANFYEIAQ
                     TAWPASLARTS"
     gene            complement(4363..4806)
                     /locus_tag="VIG70_16770"
                     /pseudo
     CDS             complement(4363..4806)
                     /locus_tag="VIG70_16770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(4823..5572)
                     /locus_tag="VIG70_16775"
     CDS             complement(4823..5572)
                     /locus_tag="VIG70_16775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8252253.1"
                     /translation="MIAQEQALVLEGVTRNFGALRAIENVSFSVAAGERRAVIGANGA
                     GKTTLFNVITGDFPATAGRVLFFGEDVTLHPPFERIRSGLRRTYQSSLLFRDLTVRDN
                     LFLAVRGVTRNRYSFFRSGNDANVEQLLERVRLTRFADQPVASLSHGQQRQLEVGMAL
                     AGAPRLILFDEPAAGLSPAERAELVALLASLPAHMGYILIEHDLEIALRVVSQVTVMH
                     NGRILKHGTPAEIENDAEVQSIYIGGKQLDS"
     gene            complement(5569..6816)
                     /locus_tag="VIG70_16780"
     CDS             complement(5569..6816)
                     /locus_tag="VIG70_16780"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8252254.1"
                     /translation="MRRTFVKTVVAAAVVAAFGGIAYAQQGKPVKIGLLATLEGPFAA
                     GGADGMRGAELAVNERKGMVAGRKIELIKASSDAKPDVAVNATRKLVEQDKVDIMVGP
                     LSGGEGIAVKEYSKTQPNITFINGSSGAQATTLVNPSPNFFRFNTEGAQWMVGLGKAA
                     LDKGYKRVMVIAEDYAFPYSQVQGFMAEYCKLGGKVPVKAWVPLGGKDYSSVIARIPK
                     DVDALVVVLGGADAVNFLNQYEAAGGDKPMIGGSITVSQDILNYKGKRRDSLVGTIAG
                     SPIADSYDAPEFKKFVADYQKAYPVSSGAYPSPSLFALVYYVNMKAALDGLEAVKGDL
                     SDGQKKYQAALQKMTLKTPTGDVRLDGNRQAIGSTFITEVVKDKQGNLFNKVLRKVDG
                     IDQMLGMKKEEFQMGSRDVPNCP"
     gene            complement(6813..7922)
                     /locus_tag="VIG70_16785"
     CDS             complement(6813..7922)
                     /locus_tag="VIG70_16785"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8252255.1"
                     /translation="MSAANWRNAMNSGAGRAAMMNRGQAAVALPLPGVGGNRGLSLIS
                     GIGARHVVVLAFLLAYPWFATPFFTFQIGAQSLALGLIALSLTFLGGYGGMVSLAQIT
                     VAGIAGYGVAIFGAASAADTAISLGWPWWVAVLISIVIATAGATFIGWLSVRTEGIYT
                     IMITLAVGVAFYYLCLQNYSVFNGFQGFQRVHAPTVLGVSMRDPLPFYYLALFWSLAG
                     YFFVKYLLRAPFGIALQGIRDNPRRMNALGFNVTAHRIAAYAVAGVMAAVGGVLLVWY
                     NGLIGPGSVGTSWIINILIIAVLGGMRHPIGAFLGAIVFVLLQTFAIDVLDRERFNLV
                     IGAVFLVIVLFSPDGLLGWWAGLKSKFLTPRGGFR"
     gene            complement(7919..8866)
                     /locus_tag="VIG70_16790"
     CDS             complement(7919..8866)
                     /locus_tag="VIG70_16790"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8252256.1"
                     /translation="MRKGWKWFWTLVIGLPLIVFVIWGLWDNTRLFVVTFLNGMTLAS
                     LYFIVAAGFTLVFGLMRNVNLAHGSLYLLGGYVGYLVAHYTGWFVLALAAGFAVAALV
                     GLLMQVSVFRFMPGEDLRQTLVTIALSIIIADLLLWWLGGTVHQADAPELLSGPLRDI
                     PLIKAYSKYRLALLPIGIAIGVVLWLFLNRTRVGMMIRAGVDDRAMLSAAGVNVNVLF
                     AVTFAIGAGLAGFGGVIGAVELSMAPGEDVRLLLASLIVVIIGGMGSVVGAALGASIL
                     GLAETYGLAYAPTYSVVFTFVILVLVLAFRPRGFLGRPA"
     gene            complement(8952..>9189)
                     /locus_tag="VIG70_16795"
     CDS             complement(8952..>9189)
                     /locus_tag="VIG70_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8252257.1"
                     /translation="MTPMEALISATRWGGQMMMKKDLGEIREGWLADLLLVDGDPLSN
                     ISVLQDKNRLLAIMKDGEFCKMPEIRSQRSRFVA"
BASE COUNT         1537 a         3091 c         2981 g         1580 t
ORIGIN      
        1 gttgccgtcg gtgaccggcg tgagcttgag cgtggcgacg tagttctcca cgcccatcgg
       61 gctctcgagg atcgagtagc tgcactggta gtcgtagtcc gagagcgtga ggagctgctc
      121 gcggatcacg ccgccgtcgc gcaggcggaa gttgcgcacg cagccgatcc tgtccgccgg
      181 catgccgttc tcgatgcgcg attcggcgac gaacggcgtc cacttcggca ggccgttgaa
      241 gtcgcgcacc tgcgcccaga ccgtgtccgc cggggcgtcg atgacgctcg aggtgtacac
      301 cttgatcaag gcttcttgcc ctcgagcaac gcattggccg cgccgccgag gtcgccgccc
      361 tgcacgccga tctcacgcag gagctggtcg acgagcggcg cctgggcgcg gaagcgcagc
      421 gcggaattca ccacgctgtc ggcgatgttg ccgccgccgt tgccgttgcc cggcccgctc
      481 tggccgccgc ccagcccctc gacgtgcagc acgcggatgc cttcgatgcg ctccatcggc
      541 ttcaccgact cgcggatgat gccctcgagc ttgtcgatca actgccggcg catcgccgac
      601 tggcgcgctt ccggcgtgag caggttggcc gccttgttca tcagcgtcag cccctcggcc
      661 tccacctcgt ggcggatctt cgccgccagc gagcggatgc gggaggtgtc ggcctccgct
      721 tcggcgttgg agacatcgat cagctttttc ctttgcgcga gctcgacctc ccgcacggtg
      781 aataccttct cctcctcggc caccgccttg gcgcgcgcat ggtcggccgc ggcctgcgcc
      841 tcggactggg ccttcgagtg ctcggcgacc gcgatcgcgc gctgctgctc ggcgagctcg
      901 atggtctgtc gacggccgat ttccagttcc tggatgccgc gttcgcgctc aatgcgctcc
      961 tgctcgaccg cgcgctcgga cacgatgcgc gcgcgctcga ccgcctcgcg cgccgtgatc
     1021 tgcgcgcgtt cggcttcctg ctcgctggtc gccttctggc gcgcgacgtc ggcgcgctgc
     1081 accgcccgct tcacctcgag ctcgcgctcc tgggcgaggc gcgcgtattc gagatcgcga
     1141 tcgatatcga gcgcctggcg ctcggtatcg aggttcttgt tgcggatctg gatcatcgtg
     1201 tcctgctcga tgtcgttcct cttcttcttg cgcgtctcga tcgtctcggt cagccgcgtc
     1261 aggccttcgg cgtcgaaggc gttcgacggg ttgaagaatt ccatcgccgt ctggtcgagc
     1321 tgcgtgagtg acacggtttc gagctcgagg ccgttctgca gcaggtccgc cgcggccgcg
     1381 gcgcgcacgc gcttcacgta ttcgccgcgc ttctcgtgca tctcttccat ggtcatctcg
     1441 gccgccacgg tgcgcagcgc gtcgacgaac ttgccctcga ccagctcctt gagcgactcg
     1501 ggctgcatgg tgcgctggcc gagcgtctgc gccgcggcgg cgatcgcctc ggcctcggcc
     1561 ttcacgcgca cgtagaactc gctcgtcacg tcgacgcgca tgcggtcctt ggtgatgagc
     1621 gcgcgctcgc gcccgcgcga cacctcgagg cgcagcgtgt tcatgttcac cgggatgacc
     1681 tcgtggatga tcggcagcac gaacgcgccg ccgttcacca ccaccttctg gccgccgaag
     1741 ccggtgcgca cgaatccccg ttccttcgag gagcgcaggt acagccagtt gagcagccag
     1801 accacgatcg caacgacgat cgcgacgccg atcagggcga ggatgaattg tccgaactgg
     1861 gatccagtca tgatgcgact cctctcttca tgcctttgaa cttcctcgtc gcctctgtca
     1921 gggcgacttc ggtgggaagg cgctccatgc tcgaagcgcc gtagaaaccg ttgcagccgc
     1981 ggcagtggtc caggatgtac tgcgcgtcct ggggcgtggc gaccggcccg ccgtggcaca
     2041 gcaccaggat gtccgggttc acgccgcgcg ccgcctgcgc ccatgcattc accaagggaa
     2101 tacattgatc cagacccaag gcggtgcgcg ccccgatggc gccgccggcg gtcagcccca
     2161 gatggcagac cacgatgtct gcgccggcct tggccatgtc gcgcgcgttc tgatccgaaa
     2221 agacataggg cgtggtgagc aggtccatct cacgcgcctg ccggatgacc tcgacctcga
     2281 gcccatagcc catgccggtt tcttcgagct gggcgcggat cgtgccgtcg aacaggccca
     2341 cggtcgggaa gttctggatc ccggagaagc ccaggtcaca gagccttttc aaaaacgatt
     2401 caaccaacag aaaaggatcg gtgccgttga ctcccgccag caccggcgtg tgcttcacca
     2461 ccggaagcac ctcgcgcgcc atgtcgagca cgatctcgtt ggcgttgccg taggccatca
     2521 ggccggcaag cgagccgcgc ccggccatgc ggtagcgccc cgagttgtag atcacgatga
     2581 gatcgatgcc gcccgcctcc tcgcacttgg cggacaggcc ggtgccggcg ccgccgccga
     2641 cgatcggctc gccgcggcgc gcctgcgcgt gcagcttctt caggatgtcc tggcgagcga
     2701 tgcgggccat gcagtttgcg cgatctcgta gaagttggcg accagcgctt cggcgaacgc
     2761 cgcgtcgttg atgtggtggg gaagccggac gagcttgcgg tttgccgtcg aacggaaatt
     2821 cgaatggatg gcttcaaaaa gcgtcttgtc ggccgccggg tcccagaacg gcttgcccgg
     2881 cgtatcgatc cccgagacgc cgccatccgg gatcaggaag cgcaccgggc cctgcatgcg
     2941 gttcaacttt tccgcgatga agcggccgat gcgcgcgttt tcctcgggcg tggtgcgcat
     3001 cagggtcacg ttgttgttgt gacgatagag attgcgctgc ttgaactgct ggggcacggt
     3061 gtccatcgcc cagaaattca ccatgtcgag cgcgccgcag gagccgacat agggaatctg
     3121 cttcctggca aaaacatcca tccgcgtcgg cccggcggtc aacacacctc cgaccagctc
     3181 gtcggcgatc tcggtggtgg tgacgtcgag cacgcccgcg atcaagccgg aatccaccag
     3241 cttctccatc gactggccgc cggtgccggt ggcgtggaac acgatgcagt cgaagcgcgc
     3301 ctcgagctgc ttgcacaccg cctgcacgca gggcgtggtc aggccgaaca tggtcaggcc
     3361 cagagcgggc ttggtttgct cctgctcttt cttggcgtag gcaatcatcc ccgccagcgc
     3421 gtgcgccgcg ttggcgagca ccttcccgga gatgcggttg atcccggaga tgtcggtgac
     3481 cgagtacatc atgcagatgt cggaaggccc gacgtagggc ttgacgtcgc ccgaagccac
     3541 ggtcgagacc atcactttcg ggatcccgat cggcagccgc cgcatcgcct gcgtcgcgag
     3601 cgcggtcccg cccgagccgc cggcggagat cagcccgccg aggtcgcggc gcagcgcgac
     3661 gaagttctcg aacgccactc ccatctcgct caccgccgag ccgcgatcgc cggtaaacac
     3721 cgcgcgctcg cccttggggt gatgccgcgc cacctcgcgc gggtggatgt ttgcggatga
     3781 cggcttgccg gaagtggcga ggtcgaccgt caccgtgcgc agaccgagct tctccaggca
     3841 gctcttgagg tagaaaagct cgcgcccctt cgtgtcgaaa gtgcctgcga tgtatgcggc
     3901 gcggcccgcc gtctgtgcga cggggaattc gacgacacgc gcctcgggct tcggttcttc
     3961 aacgggaagc ggattcctgg aaacacccca acgggtagtc ccgccgacgc tgcgctcgcc
     4021 gtcgtccggc ggcaccagct cgtcgccggc gcgcttcacg gtaagaattt ggaccggttc
     4081 cggcgcaggc gcttcctcgg caacccgaac ggcctcgtct tccgccatct tcacgccgag
     4141 gagccgctgc tgcagctcgc ggtcggcggc gagctcggcg gccggcatgg agcgcgcgat
     4201 gcggccgttc accatcacgt cgatggtgtc ggcgacgtcg atcgccacgc cgaggttctg
     4261 ctcgatgaga agcaccgcga tctcgccctc ggcggcgagc gatttgagca tggccgagac
     4321 ctgctgcacg atgaccgggg caaggccttc ggtcggctcg tccatcacca gcagcttcgg
     4381 attgaagagc agcgcgcgcg cgatcgcgag catctgctgc tcgccgccgg agagctcggc
     4441 gcccccattg ttttttcttt ccgaaagacg aggaaaaacc tggtagatgc gttcgacggt
     4501 ccagggccct ttgcgggcga ccaggcgcag atgctcatcg accgagagcg atggccagac
     4561 tcgccggccc tgcggcacgt agccgatgcc cttgtcagtg atggcgttgg gcgcgagacc
     4621 gaggatctct tcacccgcga gcttgatgct gcctgccgtg gcacttcccc ctacggcgcc
     4681 gaccaggccg gtgatggcgt tgcacagcgt ggttttcccc atgccgtttc gcccgaccac
     4741 ggaaacaatt cctttgtcga acgaaagcga gactccctgc agcgcgtgcg agcgcccgta
     4801 atgcacatcg aggttcttga tctcaagaat caagctgctt cccgcctatg tagatggact
     4861 ggacctcggc gtcgttttcg atttcggcgg gcgtgccgtg cttgaggatg cgaccgttgt
     4921 gcatcacagt gacctggctc accacgcgca gcgcgatctc gaggtcgtgc tcgatcagga
     4981 tgtagcccat gtgcgccggc agcgaggcga gcagcgccac cagctcggcg cgctccgcgg
     5041 gcgagaggcc ggcggccggc tcgtcgaaca ggatcaggcg cggcgcgccg gcgagcgcca
     5101 tgccgacctc gagctggcgc tgctggccgt gcgacaggga agccaccggc tggtcggcga
     5161 agcgggtcag gcggacgcgc tcgagcagtt gttctacgtt tgcatcgttt cctgaacgaa
     5221 agaagctgta tctgttccgg gtgactccgc gcacggcgag gaacaggttg tcgcgcaccg
     5281 tgaggtcgcg gaacagcagc gaggactggt aggtgcgccg caggccggag cggatgcgct
     5341 cgaacggcgg atgcagcgtg acgtcctcgc cgaagaatag gaccctgccc gcggttgccg
     5401 gaaagtcgcc ggtgatcacg ttgaacaggg tggtcttgcc ggcgccgttg gcgccgatga
     5461 cggcgcggcg ctcgcctgcg gcgaccgaga acgagacgtt ctcgatcgcg cgcagggcgc
     5521 cgaagttgcg ggtgacgccc tcgaggacga gggcttgctc ctgtgcgatc aagggcagtt
     5581 gggcacgtcg cgcgagccca tctggaactc ttccttcttc atgccgagca tctggtcgat
     5641 gccgtcgacc ttgcgcagca ccttgttgaa gaggttcccc tgtttgtcct tcaccacctc
     5701 ggtgatgaac gtggagccga tcgcctggcg gttgccgtcg agcctgacgt cgccggtcgg
     5761 cgtcttgagc gtcatcttct gcagggcagc ctggtacttc ttctgcccgt cagagagatc
     5821 gcccttgacc gcctcgaggc cgtcgagcgc cgccttcata ttgacgtagt acacgagcgc
     5881 gaacagcgac gggctcggat aggcgccgga agacaccggg taggctttct ggtagtcggc
     5941 gacgaacttc ttgaactcgg gcgcatcgta ggagtcggcg atcggcgagc cggcgatggt
     6001 gccgaccagc gagtcgcgcc gcttgccctt gtagttcagg atgtcctggc tcaccgtgat
     6061 cgagccgccg atcatcggct tgtcgccgcc cgccgcctcg tactggttga ggaagttcac
     6121 cgcgtcggca ccgccgagca ccaccaccag cgcatcgacg tccttcggga tgcgcgcgat
     6181 caccgacgag tagtccttgc cgccgagcgg cacccaggcc ttgaccggga ccttgccgcc
     6241 gagcttgcag tactcggcca tgaagccctg cacctgcgag tacgggaagg cgtagtcctc
     6301 ggcgatcacc atcacccgct tgtagccctt gtcgagcgcg gccttgccca ggccgaccat
     6361 ccactgcgcg ccctcggtgt tgaagcggaa gaagttcggc gacgggttga cgagcgtcgt
     6421 cgcctgcgcg ccggaggagc cgttgatgaa ggtgatgttg ggctgggtct tggaatactc
     6481 cttgaccgcg ataccctcgc cgccggaaag cggtccgacc atgatgtcga ccttgtcctg
     6541 ctcgaccagc ttgcgcgtgg cgttgaccgc gacgtcgggc ttggcgtcgg acgaggcctt
     6601 gatcagctcg atcttgcggc cggcgaccat gcccttcctc tcgttgactg ccaactccgc
     6661 accgcgcatg ccgtcggcgc cgcccgcggc gaacggccct tccagcgttg cgagcaggcc
     6721 gatcttcacc ggcttgccct gctgcgcgta agcgatgccg ccaaacgcag cgacgaccgc
     6781 cgcggcgacg actgttttta cgaacgttct cctcatctga aacctcctct gggggttaag
     6841 aacttcgatt tcagacctgc ccaccagccg agcaggccgt ccggcgaaaa cagcacgatg
     6901 accaggaaca cagcgccgat cacgaggttg aagcgctcgc gatcgagcac atcgatggca
     6961 aaggtctgca gcagcacgaa cacgatcgcg ccgaggaagg cgccgatcgg gtggcgcatg
     7021 ccgcccagca ccgcgatgat caggatgttg atgatccagc tcgtacccac cgagccggga
     7081 ccgatcagtc cgttgtacca gaccagcagc acgccgccga ccgccgccat cacgcccgct
     7141 acggcgtaag cggcgatgcg gtgcgcggtc acgttgaagc cgagcgcgtt catgcggcgc
     7201 gggttgtcgc ggatgccctg gagggcgatg ccgaacggcg cgcgcaacag gtacttcacg
     7261 aaaaaatagc ccgccagcga ccagaacagc gcgaggtagt agaacggcag cgggtcgcgc
     7321 atgctgacgc ccagcacggt cggcgcgtgc acgcgctgga atccctggaa accgttgaac
     7381 acgctgtagt tctgcaggca gaggtaatag aacgcgacgc ccaccgcgag cgtgatcatg
     7441 atggtgtaga tgccctccgt cctcaccgag agccaaccga tgaaggtggc gcccgccgtg
     7501 gcgatgacga tggagatcag gaccgccacc caccacggcc agccaaggct gatggcggtg
     7561 tccgcagcgc tcgcggcgcc gaaaatcgcc acgccgtagc cggcgatccc cgccaccgta
     7621 atctgcgcga gcgacaccat gccgccgtag ccgccaagga aagtcagcga cagcgcgatc
     7681 agcccgagcg cgagcgactg cgccccgatc tggaaggtga agaacggcgt cgcgaaccac
     7741 ggatacgcga gcagaaacgc gagcacgacc acgtgccgcg ctccgatccc tgaaatcagg
     7801 gacagaccac gatttccgcc taccccgggg agcggaagcg caaccgcagc ctggccgcgg
     7861 ttcatcattg ccgcgcggcc cgcgcccgag ttcatggcgt tgcgccaatt ggccgcgctc
     7921 aagccggcct cccgaggaat ccgcgcggcc ggaaggcgag cacgaggaca agaatgacga
     7981 aggtgaacac cacgctgtag gtcggcgcgt aggccaggcc ataggtttcg gcgagaccca
     8041 aaatggatgc tcccagggcc gctcccacca cgctgcccat gccgccgatg atcacgacga
     8101 tgagcgaggc gagcagcagg cgcacgtcct cgcccggcgc catcgagagc tccaccgcgc
     8161 cgatcacgcc gccgaagccg gcaagcccgg cgccgatggc gaaggtgacg gcgaacagca
     8221 cgttcacgtt cacgcctgcc gcggacagca tcgcgcggtc atcgaccccc gcgcggatca
     8281 tcatgccgac gcgcgtgcgg ttgaggaaca gccacagcac gacaccgatg gcgatgccga
     8341 tcggcagcag cgcgaggcgg tacttcgagt acgccttgat gagcggaatg tcgcgcagcg
     8401 gccccgacag cagctccggc gcgtcggcct ggtgcaccgt gccgcccagc caccagagca
     8461 gcaggtcggc gatgatgatc gagagcgcga tcgtcaccag cgtctggcgc aggtcctcgc
     8521 ccggcatgaa gcggaacacg ctcacctgca tcagcagccc gaccagcgcc gcgaccgcga
     8581 agccggccgc gagcgccagc acgaaccagc cggtgtaatg cgccaccagg tagccgacat
     8641 agccgcccag caggtagagc gagccgtgcg cgaggttcac gttgcgcatc aggccgaaca
     8701 ccagcgtgaa gccggccgcg acgatgaagt agagggacgc gagggtcatc ccgttgagaa
     8761 aggtcaccac gaacaggcgc gtgttgtccc acaagcccca gatcacgaac acgatcagcg
     8821 gcaggccgat gaccagcgtc cagaaccatt tccacccctt tctcatgaat cagggacaga
     8881 ccactttttt tttcgccgtt gccgattcac cggaaaacgt ggtctgtccc tgtttttggg
     8941 ttgttttggc ttcacgccac gaagcggctg cgttgcgagc ggatctccgg catcttgcag
     9001 aactcgccgt ccttcatgat cgcgagcagc cggttcttgt cctgcaggac cgagatgttg
     9061 gaaagcgggt cgccgtcgac cagcagcagg tcggcgagcc agccctcgcg gatctcgccc
     9121 aagtccttct tcatcatcat ctgcccgccc cagcgcgtcg ccgagatcag cgcctccatc
     9181 ggcgtcatg
//
LOCUS       DATBSZ010000262        22043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1142985, whole genome shotgun sequence.
ACCESSION   DATBSZ010000262 DATBSZ010000000
VERSION     DATBSZ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 22043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22043
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142985"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..1148
                     /gene="dapE"
                     /locus_tag="VIG70_16800"
     CDS             21..1148
                     /gene="dapE"
                     /locus_tag="VIG70_16800"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY8252258.1"
                     /translation="MTPALDLAQALIARRSVTPEDGGCQALIAERLALAGFKCEPMRF
                     GEVSNLWARRGRSAPLLCFAGHTDVVPTGPLSEWQSDPFVPTIRDGKLYGRGAADMKS
                     SIAAFTIATERFVKERPNHSGSIAFLLTSDEEGPALDGTAKVVEALKKRGETMDYCIV
                     GEPTSVDALGDMLKNGRRGSLSGRLTVRGIQGHVAYPHLARNPVHQLAPALAELAKTQ
                     WDKGNESFPPTTWQVSNIHAGTGVGNVIPGSIEVDFNFRFSTESTDATLAQRVEAILK
                     KHGLDYSLSWVLGARPFLSPQGKLAQTVTEVCRRHTGHRAELATTGGTSDARFIIEIA
                     PEVLEIGPVNASIHKLNEHIELSQLEMLPRIYLDTLRTLLP"
     gene            1160..1993
                     /gene="prmB"
                     /locus_tag="VIG70_16805"
     CDS             1160..1993
                     /gene="prmB"
                     /locus_tag="VIG70_16805"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY8252259.1"
                     /translation="MIRKTEARFRAARLHYGHGTDNARDEAAFLVLRGLGLPFDEDLG
                     KDADPTRIEALVKKRITQRIPAAYLLNEAWLVGLPFHVDRRVIIPRSHIAFLFKDLRI
                     RPRRILDLCTGSGCLAILAARAYPKAQVDAGDISAAALEVAARNVREHRLARRVRLVR
                     SDLFEKLRGRYDLIVCNPPYVATPSMRKLPREYRYEPGIALAGGRQGLDYAGRILEAA
                     PRYLSPGGLLVCEVGENKAALERAYPRIPFVWPRPEVFTLSLSRTAGAFRTPPRQPRA
                     K"
     gene            complement(1926..3452)
                     /locus_tag="VIG70_16810"
     CDS             complement(1926..3452)
                     /locus_tag="VIG70_16810"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEY8252260.1"
                     /translation="MQVEREIKFSLTPAAARRVARRVRPAGPWRRRMVFSAYYDTANE
                     RLRRAGVALRLRRDGTRRLQTLKAESATAGLSARAEWEMPAPRGKLDLGAFPREEILA
                     ATGLDVARLASRLRPRFETRFSRRSAPLIVDGATRAEISVDRGYVAAGERREPISEVE
                     LELKSGNTASLLRYAAALAKPLGLALEFESKAERGYRLVAGEIFPPPRKWRRPRLREL
                     ATPAEAFAAVFAAALTQAGANAHGVVHGRDAEYLHQMRVGLRRLRSALLAFRDLVPKK
                     AAKPIAGRLRGLMPSLGAARDWDVFCEGLVRIGMQAPERAPAMAPLLARARAKRGGAR
                     RRARATAASRKLQTFLLRTLRWVNSEPWKARAQDSEGSLGAFAAASLERLHGKALAQA
                     QDIDWRDPGQRHRLRIRMKRLRYACDFFAASFAGAAAQPYIKRLAALQDILGDLNDIA
                     VARRLLAEIAPRGSAPGIAGAAAHTRHTLAVRERMLVISLEAAWAAFEKRRPFWKARA
                     "
     gene            complement(3456..3530)
                     /locus_tag="VIG70_16815"
     tRNA            complement(3456..3530)
                     /locus_tag="VIG70_16815"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3496..3498),aa:His,seq:gtg)
     gene            complement(3573..3649)
                     /locus_tag="VIG70_16820"
     tRNA            complement(3573..3649)
                     /locus_tag="VIG70_16820"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3613..3615),aa:Arg,seq:tct)
     gene            complement(3655..3731)
                     /locus_tag="VIG70_16825"
     tRNA            complement(3655..3731)
                     /locus_tag="VIG70_16825"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3695..3697),aa:Pro,seq:tgg)
     gene            3913..4275
                     /gene="dksA"
                     /locus_tag="VIG70_16830"
     CDS             3913..4275
                     /gene="dksA"
                     /locus_tag="VIG70_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEY8252261.1"
                     /translation="MNAKQRSHFRKILESLKSELSQEIDRTVHTMQDEATVFADPNDR
                     ASQESDMALELRNRDRERKLIKKIDETIARIDADEYGYCDSCGVEIGLKRLEARPTAT
                     LCIDCKTLDELRERQVAK"
     gene            complement(4282..4614)
                     /locus_tag="VIG70_16835"
     CDS             complement(4282..4614)
                     /locus_tag="VIG70_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252262.1"
                     /translation="MRRAGGLMLGCFLAGCAPMSDGEYSRYVDSLLLSRESVDAALEK
                     LERERFACYEGSLGFLATAPARGPAKSCSRDSSTLAIACHQFVMIEYDAATRAVRRVS
                     ETQSCATL"
     gene            complement(4624..6744)
                     /gene="pnp"
                     /locus_tag="VIG70_16840"
     CDS             complement(4624..6744)
                     /gene="pnp"
                     /locus_tag="VIG70_16840"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY8252263.1"
                     /translation="MNPIKKTFSYGSHQVTLETGEIARQAHGAVVCTMDDTVVLATVV
                     AAKEPRVGQDWFPLTVDYVEKTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPIRPLF
                     PDGFLNEVQVVATVMSLNPEVDSDIPAMLAVSAALEISGIPFNGPIGACRVGYVNGEY
                     ILNPTATQLKESKLNLVVAGTEQGVMMVESEALELPEDAMLGGVMYGHEQMQAAIQAI
                     HELAEQAGKPAWEWQPPVKNEALQARVVELVERDMREAFAVKQKQTRNTRLVDIRKAA
                     LEKLAAEAAATGTVLNVNEVKEIFFNTESKIVRSQILDGEPRIDGRDTRTVRPITVRV
                     GVLPRTHGSALFTRGETQALVTVTLGTGRDEQIIDALAGEYRERFMMHYNMPPYATGE
                     TGRMGPSKRREIGHGRLAKRALVGLLPTPEDFGYSIRVVSEITESNGSSSMASVCGGS
                     LALMDSGVPVKAHVAGIAMGLIKDGNRFAVLSDILGDEDHLGDMDFKVAGTDKGVTAL
                     QMDLKIESISKSIMKVALDQAREARLQILDIMKQAIEGSRTELSTYAPRIIKIKINPD
                     KIRDVIGKGGVVIRGIQEETGTTIEIEDDGTISIACVSAEGGEAARKRIQELTADVEV
                     GKIYEGPVLRLLDFGAIIQLLPGRDGLLHISQISHERVNAVSDHLKEGQMVKVKVLEA
                     DDKGRVRLSMKAVKEQEQAAKQNS"
     gene            complement(6814..7083)
                     /gene="rpsO"
                     /locus_tag="VIG70_16845"
     CDS             complement(6814..7083)
                     /gene="rpsO"
                     /locus_tag="VIG70_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY8252264.1"
                     /translation="MALQVVQKEQVVKQYQRSGKDTGSPEVQIALLTERINSLTEHFK
                     THVKDFHSRRGLLKLVSQRRKLLDYLKGKDADKYRGLIERLSLRK"
     gene            complement(7096..8010)
                     /gene="truB"
                     /locus_tag="VIG70_16850"
     CDS             complement(7096..8010)
                     /gene="truB"
                     /locus_tag="VIG70_16850"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY8252265.1"
                     /translation="MIDGALLLDKPLGLSSNAVLQKAKRLFGAAKAGHAGTLDPLASG
                     LLLVLFGEATKFAGPLLDADKEYLATLKLGERTATGDAEGDVLERRPVNVTDKSLLPV
                     LQRFRGEIEQVPPMHSALKHKGTPLYRLARRGQEVERSPRRVRISHLSVESFDPPSLG
                     LRVVCSKGTYIRTLAEDIGEALGCGAHLSALRRTASGRFRVEEAAALEALEKMRPQEL
                     RERLLALPALLEGLPRAELGAAEEARLRQGQALKVGGLHPGLCAVVRPDGAVIGLGTA
                     DGEGGLRALRLTQTAEKHLKSAAHMKTE"
     gene            complement(8007..8360)
                     /gene="rbfA"
                     /locus_tag="VIG70_16855"
     CDS             complement(8007..8360)
                     /gene="rbfA"
                     /locus_tag="VIG70_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY8252266.1"
                     /translation="MKKGQGRPQRLGDLIQREVSDLIRLEVRDPRVGMITITSVDVSP
                     DMSHAKVFFTILEKQKLQDTLDGLKRSAGFLRSLLARRLKMYTTPELRFAYDESVERG
                     DRLSRLIDSAVKPRK"
     gene            complement(8357..10873)
                     /gene="infB"
                     /locus_tag="VIG70_16860"
     CDS             complement(8357..10873)
                     /gene="infB"
                     /locus_tag="VIG70_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY8252267.1"
                     /translation="MAQTSVAQFASELKVPPSVLLEQLRAAGVDKKQPDDSLSEQDKS
                     RLLEYLRKAHGSTDAKNKITLTRKHTSEIKKTDASGKYRTVQVEVRKKRVFVKRDPAE
                     VAAQAVAAETPPPPPPPAPTVDPKEIELREEEARRQQQLAELQAAELREKQERERKEA
                     EQKAAEAAAAAPAPAGAPTTLHAPKAADAKTEKKPKKAKPTTVLRDDSVRRRQIKTRG
                     DSGGGVGGWHARGGGRHRPASSGGEQTTFAQPTEPLVREVAVPETITVGELAHRMSVK
                     AAEVIKALMKLGTMATINQVLDQETGIIVVEEMGHKAKAAKLDDPESYLAEPEEAVET
                     ELKPRPPVVTVMGHVDHGKTSLLDFIRTTKVAAGEAGGITQHIGAYHVETPRGVVTFL
                     DTPGHEAFTAMRARGAQATDIVILVVAADDGVMPQTVEAINHAKAAEVPLVVALNKID
                     KHEANPDKVKQELLTHGVVPEEYGGDSPVVPVSAKTGQGVDALLEQVLLQAEVLELKA
                     STDAPAKGVVIEARLDKGRGPVATLLVQSGTLKRGDIVITGAVYGRVRAMLDENGKPV
                     QEAGPSIPVEVQGLSEVPSAGEEMMVIADERKAREIANFRQGKFRDVKFAKQHATKLE
                     NMFGQMAEGEKKVLTLVIKSDVQGSQEALVHALTRLATDEVKVTVVHAGVGAITESDV
                     NLAMAARAVIIGFNARADATARKLIESAGVQVRYYNIIYDAVEEIKGALSGMLAPERK
                     EQVLGLVEVRQVFRISKIGTVAGCYVQEGLVKRSAQVRVLRDNVVIHTGDIDSLKRFK
                     DDVREVKAGFECGMSLKNFSDIKEKDQFEVFETVEVARTL"
     gene            complement(10874..12376)
                     /gene="nusA"
                     /locus_tag="VIG70_16865"
     CDS             complement(10874..12376)
                     /gene="nusA"
                     /locus_tag="VIG70_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY8252268.1"
                     /translation="MNFGRELLLQADALAREKNVTRDIVFSALEMALASALKKRYLED
                     VDVRVAIDRNTGNYNAFRRWKIVPDGTVELVAQQIPLSEASQKKADAKPEDFIEEEIE
                     PIDPRRIGAQTAKQVLLQRIRDAEREQILQDFLSRGDELVTGTVKRMERGNAVIESGR
                     LESLLPREHMIPKENLRVGDRVRAWVMKIDRQARGPQLILSRTAPQFIMKLFELEVPE
                     IEEKLLEIKSAARDPGVRAKIAVFTNDKRIDPIGTCVGMRGSRVQAVTQELAGERVDI
                     VLWSADPAQFVIGALAPAEVSSIVVDEEKHAMDVVVDEENLAVAIGRSGQNVRLASEL
                     TGWQINLMTEEESTKKQQEESGRIKSQFMEKLDVDEEVANILIEEGFSTLEEIAYVPI
                     NELNEIQAFDEDTVNELRNRSRNALLVAAIANEEKVEGIDPELLKLEGMDTQLAAKLA
                     EGGIKTRDDLADLAVDELSELTGMESEAAKKLIMAARAHWFADGEKKEAA"
     gene            complement(12387..12818)
                     /gene="rimP"
                     /locus_tag="VIG70_16870"
     CDS             complement(12387..12818)
                     /gene="rimP"
                     /locus_tag="VIG70_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY8252269.1"
                     /translation="MSLENVVQPVVTGMGYELVDVQASNGGRLLRLFIDKPGGVGLED
                     CAAVSRQLSRVFEVEGIDYERLEVSSPGLDRPLRKGADFARFAGRKAEVRMRTPDATG
                     RRRFVGLLRGAAAGRVEMELDGHTVALELDDVERARLVPDL"
     gene            complement(12885..13586)
                     /locus_tag="VIG70_16875"
     CDS             complement(12885..13586)
                     /locus_tag="VIG70_16875"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY8252270.1"
                     /translation="MQKLLAAAGLGSRREIEGWIAAGRVSLNGQPAKLGDRAGPGDIV
                     AVDGKPVRQSGKGQPRVLLYHKPVGELVTRSDPEGRRTVFASLPPGRWIAVGRLDLNS
                     SGLLLLTDSGELANRLMHPRYGLEREYLARVRGELRPEERTRLLEGVLLDGIPARFER
                     IESDRESEGSNRWYRVVLQEGRNREVRRLFEALGHPVSRLSRVRYGPVELPRDLAAGQ
                     WRELAGKTVNGLFRQ"
     gene            complement(13591..14199)
                     /gene="scpB"
                     /locus_tag="VIG70_16880"
     CDS             complement(13591..14199)
                     /gene="scpB"
                     /locus_tag="VIG70_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY8252271.1"
                     /translation="MLNPPDVKRVLEAALLAAQEPLGATELKRLFEGELGVDAIRRLL
                     AELADEWKGRAVELVSLSSGWRFQTRPELQPYLERLFPERAPRYSRAVMETLAIIAYR
                     QPVTRGDIEDVRGVGVSTQIIQALETRGWIDVVGHRETPGRPALYATTTKFLDDLGLR
                     SLEELPPLEEIAKTLQLEVVPTAEAEAAVTDAPEQAKTGTDG"
     gene            complement(14183..14956)
                     /locus_tag="VIG70_16885"
     CDS             complement(14183..14956)
                     /locus_tag="VIG70_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY8252272.1"
                     /translation="MMAKLYGEPVFELPHDLYIPPDALEIVLEAFEGPLDLLLYLIRK
                     ENLDILDIPMAPLTRQYLEYVEAMRKTNLELAAEYLVMAAMLMEIKSRLLLPRPPAAE
                     VVEEDPRAELVRRLLEYERMKKAAQGLDELPQVGRDVIAISVWIEKTVSQRLPEVNSG
                     DLAEAWRTLLHRARLSAHHRVSREELSVRAHMSAILRALKERRVVEFAELFEPRRGVS
                     VLVVTFLALLELARELLVEITQSAGFGPIYVKLGHAQSA"
     gene            complement(14953..16155)
                     /locus_tag="VIG70_16890"
     CDS             complement(14953..16155)
                     /locus_tag="VIG70_16890"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY8252273.1"
                     /translation="MYETRVLSGMRPTGALHLGHYHGVLKNWVRLQAEYPCLFFVADW
                     HALTTDYEKPGDLQQTGLDMVIDWLAAGVDPGQAMLFVQSQVPDHAELHLLLSMMAPL
                     GWLERVPTYRDQQQKLADKDLTTYGFLGYPLLQSADILIYRAKYVPVGEDQVPHLEFA
                     REVARRFNHLYGREPGFEDKAKAAVKKMGSKKARLLNEVRTKFLEQGDANALARGQAV
                     LDEQQNLSRGDRERVYGWLEGTGKKILVEPEALLTEASKMPGLDGQKMSKSYGNTISL
                     REEPEVVAKKIKTMPTDPARVKRSDPGNPEKCPVWDLHKIYSNDERKAWVVKGCTTAG
                     IGCLECKQPVIDAINLELAPMRGRAQPYIDDPTLVKNIIADGCEKARRLAAETMRDVR
                     EAMGLTYG"
     gene            complement(16158..16802)
                     /locus_tag="VIG70_16895"
     CDS             complement(16158..16802)
                     /locus_tag="VIG70_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY8252274.1"
                     /translation="MHQLAQTLAIYAIPVLFAITLHEAAHGYVARHFGDMTAHAQGRI
                     SLNPGRHIDLIGTIVVPLVILFLSGYKFLFGWAKPVPVNYSALRKPRQHMAWVAAAGP
                     GANLVMALVWAVLLKVAVLLPAGGVSEFLALMAQAGILVNLVFMFLNLLPILPLDGGR
                     ILASLLPSRAAWQYARLEPLGLPLLVILLLTNVLNVVLEPLVGVSDALIRAIVL"
     gene            complement(16833..17459)
                     /locus_tag="VIG70_16900"
     CDS             complement(16833..17459)
                     /locus_tag="VIG70_16900"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY8252275.1"
                     /translation="MAQFFSIHPTHPQKRLVRLAADIVRRGGLIAYPTDSCYALGCDR
                     GSASGAQRLRRLRGIDERHHLTLMCRDLSEMARYAIVDDAQFKLLKSVTPGSYTFILR
                     ARREVPRRLTRKKTIGVRVPGHPVAHALLAELDQPMLSATLSLPGLATPLSDPQDIRE
                     KLEHELDLVIDGGSCGVEPSTVIDLTEGTPRVLREGRGSLAPFAVERV"
     gene            complement(17459..18328)
                     /locus_tag="VIG70_16905"
     CDS             complement(17459..18328)
                     /locus_tag="VIG70_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8252276.1"
                     /translation="MRQSESQFLQVRGLRYHVRRWPARTAPKMVLLHGWMDVSASFQF
                     VVDALERDWDVYAPDWRGYGLSEWGKADSYWFPDYLADLDVLLESIDPENPVNLVGHS
                     MGGHVAGLYAGVRPGRVARFVNLEGFGLAATRADQAPKRYTRWLDELRVPPRFRPYES
                     YAALAERLRGNNPRLSREKADFLARQWGEEVEGKGVRLRGDPAHKIVNPVLYRHDEVR
                     ACWQQATAPVLWVEATESDALKRVGLDAAAHAERRASFRTLRHVTVAGSGHMLHHDQP
                     QAVAGLLEEFLAR"
     gene            complement(18325..19554)
                     /locus_tag="VIG70_16910"
     CDS             complement(18325..19554)
                     /locus_tag="VIG70_16910"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEY8252277.1"
                     /translation="MKIIVLGAGVVGVASAWYLAAAGHEVTVIERRDAAGLETSFANG
                     GQIAAGHAEPWAKPAVVPKILRWLGREDAPLLFRPRASWAQWAWGARFLYECLPGRFE
                     RHSRTLAGLAGYSRECLRALRAELGLRYDHLERGILQFATNQRDLEAMSRHAEATRQQ
                     IKSASECHAIEPALRYSNDPVVGGVYNPNDESGDAWRFTQELARIAAARGVAFRFDAD
                     IEAIEAEAGSVRGIRLKGAPSATADAYVVSLGSYSPLLLGPLGVRIPVYPMKGYSITL
                     PLGPAEQGAAPTVSLTDEAFKIVISRLGNRLRAAGTAELTGYDTTLNSARCAAIARRI
                     RDLFPALGAVTTVENWTGLRPATPNNVPVIGKTRLRNLYLNTGHGTLGWTLACGSGSV
                     LADLISGRRPQVAFPFT"
     gene            19572..20099
                     /locus_tag="VIG70_16915"
     CDS             19572..20099
                     /locus_tag="VIG70_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY8252278.1"
                     /translation="MPLFAIGERRVQLLGEHHYIAPDASVIGSVVLENDVSVWFKVVI
                     RAENDRITIGEGCNIQDASVLHVDPGYPMTLGKHVSVGHKVMLHGCTIGEGSLIGINS
                     VIMNGARIGRGTLIGANTLIAEGKEIPEGVLVLGSPGKIVRELKQEERDYLLKVARGY
                     VDRSRFYKANLKEIT"
     gene            complement(20092..20853)
                     /locus_tag="VIG70_16920"
     CDS             complement(20092..20853)
                     /locus_tag="VIG70_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY8252279.1"
                     /translation="MQIRNSIFLVTGGASGLGAASARMAAENGGKVVIADMQAEAGEK
                     LAKELGGRFVKTDVTSEAEGKAAVAAALKEFGGLHVLVNCAGIALAERTLGKEAPHDL
                     QRFIRVLNVNLIGTFNMIRLAADAMAKAVPNASGERGVIVNTASVAAFDGQIGQAAYS
                     ASKGGVVGMTLPIARDLSRNGIRVCTIAPGIFETPMLMGLPKDAQESLGKQVPFPSRL
                     GRPAEYAQLVKAIIEVEMLNAETIRLDGAIRMAPK"
     gene            20893..>22043
                     /locus_tag="VIG70_16925"
     CDS             20893..>22043
                     /locus_tag="VIG70_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252280.1"
                     /translation="MQLGFDIRFNDLYERAGLLRVDAAFLRFLREVDGTLHDRLLAAR
                     ERPLEGKPESDLLVALAPHVEDFLPRLFGIEAEARALAERHNELAPLYSVKRLFVQRR
                     ALHKVKLEDARPDGFQFTTELDFARQVTDWLKDEAANGEKLEQAARYAAWATLTPEGK
                     EKHRHGVLFKASRKLDYMRLIPHTEPRQRRGFALTDPGTDLVGALDQANYCIWCHEQG
                     KDSCSKGFKEKAPAEGFRKTVFGVPLAGCPLEEKISEFHLVKARGEPLAALGIIAVDN
                     PMVAATGHRICNDCMKSCIYQKQEPVNIPQAETRTLKDVLELPWGFEIYSLLTRWNPF
                     DLRRPYPKASSDKRVLVVGLGPAGFTVAHHLMNDGHTVVGIDGLKIEPL"
BASE COUNT         3441 a         7631 c         7154 g         3817 t
ORIGIN      
        1 ctccttcagc gacttcaaga ttgacgccag cgcttgatct tgcccaggcg ctgatcgcgc
       61 ggcgctcggt cacccccgaa gacggcggct gccaggcgct catcgccgag cgacttgcgc
      121 ttgccggctt caagtgcgag ccgatgcgct tcggcgaggt cagcaacctc tgggcgcggc
      181 gcggccgcag cgcgcccctg ctctgttttg ccggccatac cgacgtggtg cccaccgggc
      241 cgctctccga gtggcagtcc gacccgttcg tgccgacgat ccgcgacggc aagctttatg
      301 gccgcggcgc cgcagacatg aaatcctcca ttgccgcctt caccatcgcc acggaacgtt
      361 ttgtgaagga acggccgaac cattccggct cgatcgcctt tctcctcacc tccgacgagg
      421 aaggcccggc gctcgacggc acggcgaagg tcgtggaggc gctgaagaag cgcggcgaga
      481 cgatggacta ctgcatcgtc ggcgagccga cgtcggtgga cgcgctcggg gacatgctca
      541 agaacggcag gcgcggttct ctttccggcc ggttgacggt gcgcggcatc caggggcatg
      601 tcgcgtaccc gcacctcgcc cgcaacccgg tgcaccagct tgcgcccgcg ctcgccgaac
      661 tggcgaaaac acagtgggac aaaggcaacg agagctttcc gccgacgacc tggcaggtat
      721 cgaacatcca tgccggcacc ggcgtcggca acgtcatacc cggcagcatt gaggtcgatt
      781 tcaatttccg cttctccacc gaaagcacgg acgccacgct cgcgcagcgc gtcgaagcga
      841 tcctgaagaa gcacgggctg gattactcgc tgtcgtgggt gctcggcgca cggccatttc
      901 tcagcccgca gggcaagctc gcgcagacgg tcaccgaggt ctgccggcgg cacacgggac
      961 accgggccga gctcgcgacg accggcggta cctcggatgc ccgcttcatc atcgagatcg
     1021 cgcccgaggt gctggagatc ggcccggtca acgccagcat ccacaagctg aacgagcaca
     1081 tcgagctctc gcagctcgag atgctgccgc gcatctacct cgataccctg cgcaccctgc
     1141 ttccttgacg ctggcgcagt tgatccggaa aacggaagcg cggttcaggg cagcgcgcct
     1201 ccactacggc cacggcaccg ataacgcccg cgacgaggcg gccttcctgg tgctgcgcgg
     1261 ccttggcctg ccgtttgatg aagacctggg caaggacgcc gatccgaccc ggatcgaggc
     1321 actcgtcaag aaaagaatca cgcagcgcat cccggcggcc tacctcctca acgaagcgtg
     1381 gctggtcggc ctgcctttcc atgtggatcg ccgcgtaatc attccgcggt cgcacatcgc
     1441 gttcctcttc aaggaccttc gcatcaggcc gcggcgcatt ctcgacctgt gcaccggctc
     1501 ggggtgcctg gcgattcttg ctgcacgcgc ctatccaaag gcgcaggtcg acgcgggcga
     1561 catttccgcg gcagcgctgg aagtcgccgc ccgcaatgtc cgcgagcaca ggctggccag
     1621 gcgcgtgcgc ctcgtgcgtt cagatctctt cgagaaactc agaggccgtt acgacttgat
     1681 cgtgtgcaac ccgccctacg tagccacgcc ctcgatgcgc aagctgccgc gcgagtaccg
     1741 ctacgagccg ggaatcgccc tcgccggcgg ccggcaaggg ctggactacg ccgggcgcat
     1801 cctcgaggcg gcgcctcgat atctcagccc gggtgggctg ctggtgtgcg aggtaggcga
     1861 gaacaaggcg gcgctcgagc gcgcctatcc gcgcatcccc ttcgtgtggc cgcggcccga
     1921 ggtgttcacg ctctcgcttt ccagaacggc cggcgctttt cgaacgccgc ccaggcagcc
     1981 tcgagcgaaa tgacgagcat gcgctcgcgc acggcgagcg tatggcgcgt atgcgcggcg
     2041 gcgcccgcaa tgccgggcgc gctgccgcgc ggagcgatct ccgcgagcag gcggcgcgct
     2101 accgcgatgt cgttgaggtc gccgaggatg tcctgcagcg cggcgaggcg cttgatatag
     2161 ggctgcgccg ctgcgccggc gaacgacgcg gcgaagaagt cgcaggcgta gcgcaggcgc
     2221 ttcatgcgga tgcgcaggcg gtgacgctgc ccgggatcgc gccagtcgat gtcctgcgcc
     2281 tgggcgagcg ccttgccgtg cagccgctcg agcgacgctg ccgcgaaagc cccgagcgag
     2341 ccttcggaat cctgcgcgcg cgctttccac ggctcggagt tgacccagcg caatgtccgc
     2401 aggagaaagg tctgcagctt gcgcgacgct gcggtcgccc gcgcgcgccg ccgcgcgccg
     2461 ccgcgctttg cgcgggcgcg cgccaggagc ggcgccatcg ccggcgcacg ctcgggcgcc
     2521 tgcattccga tgcgcaccag gccttcgcaa aacacgtccc agtcgcgcgc ggcgccgagg
     2581 gaaggcatca ggccgcgcag gcgaccggcg atcggcttcg ccgccttctt cggcacgaga
     2641 tcgcggaagg cgagcagcgc ggagcgcagc cgccgcagcc cgacgcgcat ctggtgcagg
     2701 tattccgcgt cgcggccatg cacgacgcca tgggcgttcg ctccggcctg cgtcagcgcc
     2761 gcggcgaaga cggcggcgaa cgcctcggcg ggcgtcgcga gctcgcgcag cctcggccgg
     2821 cgccatttgc gcggcggcgg aaatatttcg ccggcgacca gccgatagcc gcgctcggcc
     2881 ttgctttcga actccagcgc tagtccgagc ggcttggcga gcgcggccgc atagcgcagc
     2941 agcgatgccg tgttgccgga cttgagctcg agctcgactt cgctgatcgg ctcgcgccgc
     3001 tcgccggcgg ccacgtagcc gcgatcgacc gagatttccg cgcgcgtcgc gccgtcgacg
     3061 atcagcggcg ccgagcggcg cgagaagcgc gtctcgaagc gcggccgcag ccggctcgcg
     3121 aggcgcgcga catccagccc ggtcgccgcc aggatttcct cgcgcggaaa cgcgccgagg
     3181 tcgagcttgc cgcgcggcgc gggcatctcc cattccgcgc gcgcgctcag gcccgcggtc
     3241 gcggactccg ctttcagcgt ctgcagccgg cgcgtgccgt cgcgccgcag cctgagcgct
     3301 acgcccgcgc gccgcagccg ctcgttcgcg gtgtcgtaat acgcgctgaa gaccatgcgt
     3361 cgccgccacg ggcccgcggg ccggacccgg cgagcgacgc gccgcgccgc cgcgggcgtg
     3421 aggctgaact tgatttcacg ctcgacttgc atggatgggg tgaccgaggg gactcgaacc
     3481 cccaacaacc ggaatcacaa tccgggactc tacctttgag ctacggtcac cattgttgca
     3541 agaaactgtc aacatgatcg cgcctagcgc gatggcctgc ccggcgggac tcgaacccac
     3601 aacccccagc ttagaaggct ggtgctctat ccggttgagc tacgggcagt cccgtggtcg
     3661 gggtgagagg atttgaacct ccgacatctt gctcccaaag caagcgcgct accaggctgc
     3721 gccacacccc gaaaaaggcg tcaaatgata ctcttagcgc atgcccgtcg caatcgaagc
     3781 agcttgctac agggggtctt tagtgctaca aagcgcattc gttttttgac cccgagatag
     3841 ccgaaaagaa tggccgtaga actttcccgc aaaggtttca cgccgtacgc tgcgaaaaag
     3901 ggcgaggagt acatgaacgc caagcagcgc tcgcacttcc gcaagatcct cgagtcgctc
     3961 aagtcggagc tctcgcagga gatcgaccgc acggtgcaca ccatgcagga cgaagcgacg
     4021 gtgttcgccg atccgaacga ccgcgccagc caggaatccg acatggcgct cgagctgcgc
     4081 aaccgcgacc gcgagcgcaa gctgatcaag aagatcgacg agaccatcgc gcgcatcgac
     4141 gcggacgagt acggctactg cgacagctgc ggcgtcgaga tcggcctcaa gcgcctggaa
     4201 gcgcgcccga cggcgacgct gtgcatcgac tgcaagacgc tcgatgagct gcgcgagcgg
     4261 caggtcgcga agtaataccc cctacaaggt cgcgcaggac tgcgtttcgc tgacgcgccg
     4321 gacggcgcgg gtggcggcgt cgtattcgat catcacgaat tgatggcacg cgatggcgag
     4381 cgtcgagctg tcgcgagagc agctttttgc gggacctcgc gccggcgccg tcgccaggaa
     4441 tccgagcgaa ccctcgtagc aggcgaatcg ctcgcgttcc agcttctcca gcgccgcgtc
     4501 gacgctctcg cgcgatagca gcagcgagtc cacgtaccgg gaatactcgc cgtcgctcat
     4561 cggggcgcac cccgccagga agcagccgag catcagccct cctgcgcggc gcaagcccgg
     4621 cgcctagctg ttctgcttcg cggcctgctc ctgctccttg acggccttca tcgacagccg
     4681 cacgcgtccc ttgtcgtcgg cctcgagcac cttgaccttc accatctggc cttccttgag
     4741 gtggtccgac acggcgttga cgcgctcgtg gctgatctgc gagatgtgca gcaggccgtc
     4801 gcggccgggc agcagctgga tgatggcgcc gaagtcgagc aggcgcagca ccggcccctc
     4861 gtagatcttg ccgacctcga cgtccgcggt cagctcctgg atgcgcttgc gcgccgcctc
     4921 gccgccctcc gccgacacgc acgcgatcga tatcgtgccg tcgtcctcga tctcgatggt
     4981 ggtgccggtc tcctcctgga tgccgcggat cacgacgccg cccttgccga tgacatcgcg
     5041 gatcttgtcc gggttgatct tgatcttgat gatgcgcggc gcgtaggtcg acagctcggt
     5101 gcgcgagccc tcgatcgcct gcttcatgat gtcgaggatc tgcagccggg cctcgcgcgc
     5161 ctggtcgagc gcgactttca tgatgctctt gctgatcgac tcgatcttca ggtccatctg
     5221 cagcgcggtg acgcccttgt cggtgccggc gaccttgaag tccatgtcgc cgagatggtc
     5281 ctcgtcgccg aggatgtccg acagcacggc gaagcggttg ccgtccttga tcaggcccat
     5341 ggcgatgccg gcgacgtgcg ccttgaccgg cacccccgag tccatcagcg cgaggctgcc
     5401 gccgcacacc gacgccatgg aggaagaccc gttcgactcg gtgatctcgg acaccacgcg
     5461 gatcgagtag ccgaaatcct cgggtgtcgg cagcaggccg acgagcgcgc gcttggcgag
     5521 gcggccgtgg ccgatctcgc gccgcttcga cgggcccatg cggccggtct cgccggtggc
     5581 gtagggcggc atgttgtaat gcatcatgaa ccgctcgcgg tattcgcccg cgagcgcgtc
     5641 gatgatctgc tcgtcgcgac ccgtgccgag ggttacggtt acgagcgctt gcgtttcccc
     5701 tctggtaaac aaggcagaac catgagtgcg cggcagcacc cctacgcgca ccgtgatcgg
     5761 cctcaccgtg cgtgtgtctc tgccgtcgat gcgcggctcg ccgtcgagaa tctggctgcg
     5821 cacgatcttc gattccgtgt tgaagaagat ttccttcacc tcgttgacgt tgagcaccgt
     5881 cccggtcgcg gcggcctcgg cggccagctt ctcgagcgcg gccttgcgga tgtcgacgag
     5941 gcgcgtgttg cgcgtctgct tctgcttcac ggcgaaggcc tcgcgcatgt cgcgctcgac
     6001 gagctcgacg acgcgcgcct gcagcgcctc gttcttcacc ggcggctgcc attcccacgc
     6061 cggcttgccg gcctgctcgg cgagctcgtg gatcgcctgg atcgcggcct gcatctgctc
     6121 gtgcccgtac atcacgccgc cgagcatcgc gtcctcgggc agctcgagcg cttcggactc
     6181 gaccatcatc acgccctgct cggtgccggc gacgaccagg ttgagcttcg actccttcag
     6241 ctgcgtcgcg gtcgggttga ggatgtactc gccgttcacg tagccgacgc ggcaggcgcc
     6301 gatcgggccg ttgaacggga tgcccgagat ctcgagcgcc gcggacaccg cgagcatcgc
     6361 cgggatgtcg gaatcgacct cggggttgag cgacatcacc gtcgccacga cctggacttc
     6421 gttcaggaag ccgtccggaa agagcggccg gatcggccgg tcgatcagcc tcgaggtcag
     6481 ggtctccttc tcggagggcc ggccctcgcg cttgaagaag ccgccgggga tcttgcccgc
     6541 ggcgtaggtc ttctcgacgt agtcgacggt gagcgggaac cagtcctggc cgacgcgcgg
     6601 ctccttggcg gcgaccacgg tggcgaggac gacggtgtcg tccatggtgc agaccaccgc
     6661 gccgtgcgcc tggcgcgcga tttcgccggt ctcgagcgtt acctggtgac tgccgtagga
     6721 aaaggtcttc ttgatggggt tcacgggggt ccttgaaagc aaaaagcgct catccagcga
     6781 tgagcgctcg gcctgaagac atgcgccggg ggtctatttg cggaggctca gccgctcgat
     6841 gaggccgcgg tacttgtccg cgtccttgcc cttcagataa tcgagcagct tgcggcgctg
     6901 gctcaccagc ttcaggagcc cgcggcgcga atggaagtct ttgacgtgcg tcttgaaatg
     6961 ctcggtgagg ctgttgatgc gctcggtgag gagcgcgatc tgcacctcgg gcgagccggt
     7021 gtccttgccg gagcgctggt attgcttgac cacctgctcc ttctgtacga cctgcaatgc
     7081 catctgtgat ctctcttact cggtcttcat gtgagccgcg gattttaaat gtttttccgc
     7141 cgtttgggtg agccttagcg ccctcaagcc gccttcgccg tcggcggtgc ccagcccgat
     7201 aaccgcgcca tcaggccgca ccaccgcgca cagccccggg tgcagtccgc cgaccttcag
     7261 cgcctgccct tgccgcaggc gagcttcctc cgcagcgccg agctcggctc gcggcaaccc
     7321 ctcgaggagc gcgggcagtg cgagcaggcg ctcgcgcagc tcttgcggac gcatcttctc
     7381 cagtgcctcc agcgcggccg cctcctcgac gcgaaagcgc cccgatgccg tgcgccgcag
     7441 cgcggacaga tgcgcgccgc agccgagcgc ctcgccgatg tcctcggcga gcgtgcggat
     7501 ataggtgccc ttgctgcaca ccacccgcag gccgaggctg ggcgggtcga agctctcgac
     7561 cgacaagtgc gagatgcgca cgcggcgcgg tgagcgctcg acctcctgcc cgcggcgcgc
     7621 caggcggtag agcggcgtgc ccttgtgctt cagcgccgaa tgcatcggcg gcacctgctc
     7681 gatctcgcca cggaaccgct gcagaaccgg cagcagcgac ttgtccgtca cgttgacggg
     7741 cctcctctcc agcacgtcgc cttctgcatc cccggtcgcg gtgcgctctc cgagcttgag
     7801 cgtggcgaga tactccttgt ccgcgtcgag caacggcccg gcgaacttgg ttgcctcgcc
     7861 gaaaagtacc agcagcaggc ccgaggcaag cggatcgagc gtgccggcat gcccggcttt
     7921 cgccgccccg aagagccttt ttgccttctg caacacggcg ttcgagctca gcccgagcgg
     7981 cttgtcgagc agcagggcgc cgtcgatcat ttcctcggct tcacggcgga atcgatcagg
     8041 cgcgacagac ggtcgccgcg ctccaccgat tcgtcgtacg cgaagcgcag ctcgggcgtg
     8101 gtgtacatct tgaggcgccg cgcgagcagc gagcgcagga aaccggcgga gcgcttgagg
     8161 ccgtcgagcg tgtcctgaag tttctgtttt tccaggattg taaaaaagac cttcgcatgc
     8221 gacatgtccg gcgacacgtc gaccgaggtg atggtgatca tgccgaccct cgggtcgcgc
     8281 acttccaacc ggatcagatc ggaaacttcc cgctggatca ggtcgcccag ccgctgcgga
     8341 cgaccttgtc cctttttcac agcgtcctcg caacctccac cgtctcgaag acctcgaact
     8401 ggtctttctc cttgatatcg gagaagttct tgagcgacat gccgcactcg aagcccgcct
     8461 tcacctcgcg cacatcgtcc ttgaagcgct tgagcgagtc gatatccccg gtatggatca
     8521 ctacgttgtc gcgcagcacc cgcacctggg cgctgcgctt gaccaggcct tcctgcacgt
     8581 agcaaccggc caccgtgccg atcttggaga tgcggaacac ctggcgcacc tcgaccaggc
     8641 cgagcacctg ctccttgcgc tccggcgcga gcatcccgga cagcgcccct ttgatctcct
     8701 cgaccgcatc gtagatgatg ttgtagtagc ggacctggac cccggcggac tcgatcagct
     8761 tgcgcgcggt cgcgtcggcg cgcgcattga agccgatgat caccgcgcgt gcggccatcg
     8821 ccaggttgac gtccgattcg gtgatggcgc cgacaccggc gtgcaccacc gtgaccttga
     8881 cttcgtcggt ggcaaggcgc gtcagcgcgt gcaccagcgc ctcctgcgag ccctgcacgt
     8941 cggacttgat caccagggtc agcaccttct tctcgccctc ggccatctgg ccgaacatgt
     9001 tctcgagctt ggtggcgtgc tgcttggcga acttcacgtc gcggaacttg ccctggcgga
     9061 aattggcgat ttcgcgcgcc ttgcgctcgt cggcgatcac catcatctcc tcgcccgcgc
     9121 tcggcacctc cgacagcccc tgcacctcca ccggaatcga cgggccggct tcctgcaccg
     9181 gcttgccgtt ttcgtcgagc atcgcgcgta cccgaccgta gaccgcgccg gtgatgacga
     9241 tgtcgccgcg tttgagcgtg cccgactgca ccagcagggt cgccaccggg ccgcggccct
     9301 tgtcgaggcg cgcctcgatc accacgccct tggccggcgc atcggtcgac gccttcagct
     9361 cgagcacctc cgcttgcagc agcacctgct cgaggagcgc atccacgccc tggccggtct
     9421 tcgccgacac cggtaccacc ggggagtcgc cgccgtattc ctcgggcacc acgccgtgcg
     9481 tcagcagctc ctgcttcacc ttgtcggggt tggcttcgtg cttgtcgatc ttgttcagcg
     9541 ccacgaccag cggcacctcc gccgccttgg cgtggttgat cgcttccacc gtctgcggca
     9601 tcacgccgtc gtcggccgcc acgaccagga tcacgatgtc ggtggcctgc gcgccgcgcg
     9661 cccgcatggc ggtgaacgcc tcgtggcccg gcgtatcgag gaaggtgaca acgccgcggg
     9721 gcgtttcgac gtggtaggcg ccgatgtgct gggtaatgcc gcccgcctcg cccgcggcga
     9781 ctttggtggt gcggatgaag tcgagcagcg aagtcttccc gtggtcgacg tggcccatca
     9841 ccgtcaccac cggcggccgc ggcttgagct cggtttcgac cgcttcttcg ggctcggcga
     9901 gataggactc gggatcgtcg agcttggccg ccttggcctt gtggcccatt tcctcgacca
     9961 cgatgatccc ggtctcctgg tcgagcacct ggttgatggt cgccatggtg ccaagcttca
    10021 tcagcgcctt aatcacctcg gccgctttga ccgacatgcg gtgggcgagc tcgcccaccg
    10081 tgatggtctc cggcaccgcc acctcgcgca ccagcggctc ggtgggctgg gcgaacgtcg
    10141 tctgctcacc gccgctggat gcaggccggt ggcggccgcc gccgcgcgca tgccagccgc
    10201 cgaccccgcc gcccgaatcg ccccgggtct tgatctggcg ccggcgcacc gagtcatcgc
    10261 gcagcacggt ggtgggcttg gctttcttcg gcttcttctc ggtttttgcg tcggcagcct
    10321 ttggcgcgtg cagcgtagtg ggcgcccccg cgggtgcggg cgccgccgcg gccgcctcgg
    10381 ccgccttctg ctcggcttcc ttgcgctcgc gttcctgctt ctcgcgcagc tcggccgcct
    10441 gcagctcggc aagctgctgc tggcggcggg cctcctcttc cctcagctcg atctctttcg
    10501 ggtcgacggt cggagccggc ggcggcgggg gtggcggcgt ttccgccgcg acggcctggg
    10561 ccgccacctc ggccggatcg cgcttgacga acacgcgctt cttgcgcacc tcgacctgga
    10621 ccgtgcggta cttgccgctc gcgtcggtct tcttgatctc gctcgtgtgc ttgcgcgtga
    10681 gagtgatctt gttcttcgcg tcggtcgagc cgtgcgcctt gcgcaggtac tcgagcaggc
    10741 gcgacttgtc ctgctcggag agcgagtcgt ccggctgctt cttgtcgacg ccggccgcgc
    10801 gcagctgctc gagcagcacc gacggcggta ccttcagctc gctggcgaac tgcgcaacgc
    10861 tcgtctgcgc cacttacgcg gcctccttct tttcaccatc agcgaaccaa tgggcacggg
    10921 cggccatgat cagcttcttc gcagcctcgc tctccattcc ggtcagctcg gaaagctcgt
    10981 cgaccgcgag gtcggcgaga tcgtcgcgcg tcttgatccc gccctcggcc agcttggcgg
    11041 cgagctgcgt gtccatgccc tcaagcttga gcagctcggg gtcgataccc tcgacttttt
    11101 cctcgttggc gatcgccgcc acgaggagcg cgttgcgtga gcggttgcgc agctcgttca
    11161 ccgtgtcctc gtcgaacgcc tggatctcgt tcagctcatt gatcggcacg taggcgatct
    11221 cctcgagggt cgagaagcct tcctcgatca ggatgttggc gacttcctcg tcgacgtcga
    11281 gcttctccat gaactggctc ttgatgcggc cgctttcctc ctgctgcttc ttggtcgact
    11341 cttcctcggt catcaggttg atctgccagc cggtcagctc ggaggcgagg cgcacgttct
    11401 gcccgctgcg gccgatcgcc accgcgaggt tctcctcgtc gaccaccacg tccattgcat
    11461 gcttttcttc atccacaaca atggaagaaa cctcggcggg tgccagcgcg ccgatcacga
    11521 actgcgcagg gtcggccgac cacagcacga tgtcgacgcg ctcgccggcg agctcctggg
    11581 tgacggcctg gacgcgcgag ccgcgcatgc cgacgcacgt gccgatcggg tcgatgcgct
    11641 tgtcgttggt gaacaccgcg atcttggcgc gcacgcccgg gtcgcgcgcc gccgacttga
    11701 tctcgagcag cttctcctcg atctcgggca cctcgagctc gaacagcttc atgatgaatt
    11761 gcggcgcggt gcgcgacagg atcagctgcg ggccgcgcgc ctggcggtcg atcttcatca
    11821 cccacgcgcg cacgcggtcg ccgacgcgca ggttttcctt cgggatcatg tgttcgcgcg
    11881 gcagcagaga ttccagacgg cccgactcga tgaccgcgtt gccgcgctcc atgcgcttga
    11941 cggtgccggt gacgagttcg tcgccgcgcg agaggaagtc ctgcaggatc tgctcgcgct
    12001 cggcatcccg gatgcgctgc agcaggacct gcttcgccgt ctgggcgccg atgcgccggg
    12061 gatcgatcgg ctcgatctcc tcctcgatga aatcctcggg cttggcgtcg gccttcttct
    12121 gcgaggcctc ggacagcggg atctgctgcg ccaccagctc gaccgtgccg tcgggcacga
    12181 tcttccagcg ccggaaggcg ttgtagttgc cggtgttgcg gtcgatggcg acgcgcacat
    12241 cgacgtcctc gaggtagcgc ttcttgagcg ccgaggcgag cgccatctcg agcgccgaaa
    12301 aaacgatgtc gcgcgtcacg ttcttctcgc gtgccagcgc atccgcctgc agcagcagct
    12361 ctctaccgaa attcattcag cctctctcac agatccggca ccaggcgcgc ccgctcgacg
    12421 tcgtccagct cgagcgccac cgtgtgcccg tccaattcca tctccactcg gcccgcggcg
    12481 gcgccgcgca gcaggcccac aaaccgccgc cgtcccgtgg cgtcgggcgt gcgcatccgt
    12541 acctcggcct tgcggcccgc aaagcgcgca aagtcggcgc ccttgcgcag cggccggtcg
    12601 aggcccggcg aggacacttc cagccgctcg taatcgattc cctcgacctc gaagacccgg
    12661 gacagctgcc ggctgacggc cgcgcagtcc tctaagccca ccccgcccgg cttgtcgatg
    12721 aacaggcgca acagccgtcc gccgttcgaa gcctgcacgt cgaccagctc gtatcccatg
    12781 ccggtcacca ccggctgcac cacgttttct aagctcaaac aaaaaatggg ccgaagccca
    12841 tctctgattt cacagcacca aagcctttgg attttagcaa tatctcactg acggaacaag
    12901 ccgtttacgg ttttccccgc cagctcgcgc cactgcccgg ctgccaggtc ccgcggcagc
    12961 tcgaccgggc cgtagcgcac gcgggagagg cggctcaccg ggtggccgag cgcttcgaac
    13021 aggcgccgca cctcccggtt gcggccctcc tgcagcacga cgcgatacca gcgattgctc
    13081 ccctcgcttt cgcgatcgct ttcgatgcgc tcgaaccggg ccggaatgcc atccaaaaga
    13141 acgccttcca gcaggcgcgt tcgctcctca ggcctcagtt cacccctgac ccgcgcaagg
    13201 tattcgcgct ccagcccgta gcgcggatgc atcagccgat tcgcaagctc gcctgaatcg
    13261 gtgaggagaa gcagcccgga gctgttgagg tcgagccggc ccactgcaat ccagcgtccc
    13321 ggcggcaagc tcgcaaacac cgtccgccgg ccttcggggt cgctgcgcgt gacgagctcc
    13381 ccgaccggct tgtggtacag gaggacgcgc ggctggcctt tcccggattg ccttaccggc
    13441 ttgccgtcca ccgcgacgat gtcgccgggg ccggcgcggt cgcccagctt ggctggctgc
    13501 ccattgaggc tgacccgccc ggccgcgatc cacccctcga tctcgcgccg cgagcccagg
    13561 cccgccgcgg cgagcagctt ctgcaaccgc tcagccgtcg gttccggtct tggcttgctc
    13621 tggggcgtct gtgacggcgg cttctgcttc tgcagttggc acgacctcga gctggagcgt
    13681 cttggcgatt tcctcgagcg gcggcagctc ttccagcgag cgcagcccca ggtcgtcgag
    13741 gaacttcgta gtggtggcgt aaagcgctgg ccggccaggg gtttcgcgat ggccgacgac
    13801 gtcgatccag ccccgggtct cgagggcctg gatgatctgc gtcgacacgc ccacgccgcg
    13861 cacgtcttcg atgtcgccgc gggtgaccgg ctggcggtag gcgatgatcg ctagcgtttc
    13921 catgaccgcg cgcgagtagc gcggcgcccg ttccgggaac aggcgctcca ggtagggctg
    13981 aagctccggg cgggtctgga agcgccagcc gctcgaaagg ctgacgagct cgacggcgcg
    14041 gcccttccat tcgtccgcga gctcggcgag caggcgccgg atcgcgtcca cgcccagctc
    14101 gccctcgaac aaacgtttga gctcggtcgc gcccaggggc tcctgtgcgg cgaggagcgc
    14161 cgcctcgagt acgcgcttca catcaggcgg attgagcatg ccccaatttt acgtaaatcg
    14221 gaccgaaacc agcagactgc gtgatttcga cgagcagctc gcgtgcgagc tcgagcaggg
    14281 cgaggaacgt caccaccagc accgagacgc cgcgccgcgg ctcgaacagc tcggcgaact
    14341 cgacgacccg cctttccttc aacgccctga gaatggcgct catgtgagcg cgcacggaga
    14401 gctcctcgcg gctgacgcgg tggtgcgccg agagccgcgc gcggtgcagc agcgtccgcc
    14461 aagcttccgc caggtctccc gaattcacct cgggaaggcg ctgcgagacc gtcttttcga
    14521 tccacaccga gatcgcgatc acgtcgcggc cgacctgcgg cagctcgtcg agcccctgcg
    14581 cagccttctt catgcgctcg tactcgagca ggcgccgcac cagctcggca cgcgggtctt
    14641 cctccaccac ctcggcggcc gggggccgcg gcagcagcag ccgcgacttg atctccatca
    14701 gcatcgccgc catcaccagg tattcggcgg cgagctcgag gttggtcttg cgcatcgcct
    14761 cgacgtactc gaggtactgg cgcgtgagcg gcgccatcgg gatatccagg atgtcgaggt
    14821 tttccttgcg gatcaggtac aggagcagat cgagggggcc ctcgaaagcc tcgagcacga
    14881 tctcgagcgc gtcgggcggg atgtacaggt cgtgcggcag ctcgaagacc ggctcgccgt
    14941 agagcttggc catcatccgt aggtaagtcc catcgcttct cgcacgtcgc gcatggtttc
    15001 ggccgcgagc cgccgcgcct tctcgcagcc gtcggcgatg atgttcttga ccagcgtcgg
    15061 atcatcgatg tagggctgcg cgcggccgcg catcggcgcg agctcgaggt tgatcgcgtc
    15121 gatgaccggc tgcttgcatt ccaggcaacc gatcccggcg gtggtgcagc ccttcaccac
    15181 ccatgccttg cgctcgtcgt tcgagtagat cttgtgcagg tcccacaccg ggcatttttc
    15241 ggggttgccg ggatcgctgc gcttcacccg tgccggatcg gtcggcatgg tcttgatctt
    15301 cttcgccacg acctcgggct cctcgcgcag cgagatcgtg ttgccgtagg acttggacat
    15361 cttctgcccg tcgaggcccg gcatcttcga cgcctcggtg agcagcgcct ccggctccac
    15421 caggatcttc ttgcccgtgc cctcgagcca gccgtagacg cgctcgcggt cgccgcgcga
    15481 caggttctgc tgctcgtcga gcaccgcctg cccccgcgcc agcgcgttgg cgtcgccctg
    15541 ctccaggaac ttggtgcgca cctcgttcag gagccgcgcc ttcttgctgc ccatcttttt
    15601 caccgcggcc ttggccttgt cctcgaagcc gggctcgcgg ccgtacaggt ggttgaagcg
    15661 cctcgcaacc tcgcgcgcga actcgaggtg cggcacctgg tcctcgccga ccggcacgta
    15721 cttcgcgcgg tagatcagga tgtcggccga ctgcaggagc gggtagccga ggaagccgta
    15781 ggtggtgagg tccttgtcgg cgagcttctg ctgctggtcg cggtaggtcg ggacgcgctc
    15841 gagccagccg agcggggcca tcatcgacag cagcaggtgc agctcggcgt gatcgggcac
    15901 ctgcgactgg acgaacagca tcgcctgccc cggatcgacg ccggcggcga gccagtcaat
    15961 caccatgtcg aggccggtct gctgcaggtc gcccggcttc tcgtagtccg tggtgagcgc
    16021 atgccagtcg gcaacgaaga acaggcacgg atactccgcc tgcaggcgga cccagttctt
    16081 gagcacgccg tggtagtggc cgaggtgcaa ggcgcccgtc ggccgcatgc ccgacagcac
    16141 ccgggtttcg tacatcgtca gagcacaatc gcgcgaatca gcgcgtccga gacgcccacc
    16201 aggggctcca gcaccacgtt gagcacattg gtcagcagca ggatcaccag cagcggcagg
    16261 ccgagcggct cgagcctggc gtattgccac gcggcgcggc tcggcagcag gctcgcgagg
    16321 atccgcccgc cgtcgagcgg caggatcggc agcaggttga ggaacatgaa caccaggttg
    16381 accaggattc ccgcctgcgc catgagcgcc aggaactcgg aaacgcctcc tgccggcagc
    16441 agcacggcga ccttgagcag caccgcccag acgagcgcca tgacgaggtt ggcgcccgga
    16501 ccggccgccg ccacccacgc catgtgctgc cgcggcttac gcagcgccga gtagttcacc
    16561 ggcacgggtt tggcccaccc gaacaggaac ttgtagccgg aaagaaacag gatcacgagc
    16621 ggaaccacga tcgtgccgat gagatcgata tggcgccccg ggttgaggct gatgcggccc
    16681 tgcgcgtgcg cggtcatgtc gccgaaatgg cgcgcgacgt agccgtgtgc cgcctcgtgg
    16741 agcgtaatgg caaaaagtac cgggatcgcg tagatggcca gcgtttgtgc gagctggtga
    16801 atgttgacgt ccatcttgcg gctgcgattt tatcagacgc gctctacggc gaagggcgcg
    16861 agcgaacccc ttccctcgcg cagcacgcgc ggcgtgcctt cggtcagatc gatgaccgtg
    16921 ctcggctcca cgccgcacga tccgccgtcg atgaccaggt cgagctcgtg ctcgagcttt
    16981 tctcggatgt cctgaggatc cgatagcggt gtcgccaggc cggggagcga caatgtcgca
    17041 gaaagcatcg gctggtcgag ctcggcgagg agcgcgtggg ccaccggatg cccgggaacc
    17101 cgcacgccga tggtcttttt ccgggtcagg cggcgcggga cttcgcggcg cgcgcgcaga
    17161 atgaaggtgt agctgcccgg cgtgacgctc ttcagaagct tgaactgcgc gtcgtcgacg
    17221 atcgcgtagc gcgccatctc ggacaggtcg cggcacatga gcgtgaggtg gtggcgctcg
    17281 tcgatgccgc ggaggcgccg caggcgctgc gcgccgctgg cgctgccacg gtcgcacccg
    17341 agcgcatagc aggaatcggt cggataggcg atcagcccgc cgcggcgcac gatgtccgcc
    17401 gcaaggcgga cgagccgctt ctgcggatgc gtcggatgga tcgagaagaa ctgggccatc
    17461 agcgcgcgag aaactcttcc agcaggcccg ccacggcctg cggctggtca tggtgcagca
    17521 tgtggcccga gcccgccacg gtgacatggc gcagcgtgcg gaacgaggcc cggcgctcgg
    17581 cgtgcgcggc agcgtcgagc ccgacccgct tgagcgcgtc ggactcggtc gcctcgaccc
    17641 acagcacggg cgccgtcgcc tgctgccagc aggcgcgcac ttcgtcgtgg cgatagagca
    17701 ccgggttgac gatcttgtgt gccgggtcgc cgcgcagcct gacgcccttc ccctcgactt
    17761 cctcgcccca ctgccgcgcc aggaaatcgg ccttttccct ggagagccgc gggttgttgc
    17821 cgcgcaggcg ctcggccagc gcggcatagc tctcgtaagg ccggaatcgc ggcggcacgc
    17881 gcagctcatc gagccagcgc gtatagcgct tgggcgcttg gtccgcgcgc gtggcggcga
    17941 gaccaaatcc ttccaggttc acgaatcggg cgactcgccc gggccgcacg cctgcataca
    18001 gcccggcgac gtggccgccc atgctgtggc cgacgaggtt gaccgggttt tccggatcga
    18061 tgctctcgag aagcacgtcc aggtccgcca ggtagtcggg gaaccagtag ctgtcggcct
    18121 tgccccactc gctcagcccg tagccgcgcc agtccggcgc atacacgtcc cagtcgcgct
    18181 cgagcgcatc gaccacgaac tggaaggagg ccgagacatc catccagccg tggagcagca
    18241 ccatcttcgg cgctgtgcgc gcgggccagc ggcgaacgtg gtagcgcagc ccgcgaacct
    18301 gcaggaactg cgactcgctt tgcctcatgt gaacgggaac gccacctgcg gccgccggcc
    18361 ggagatcagg tcggcgagca cgctgcccga gccgcacgcc agcgtccagc cgagcgtgcc
    18421 gtggccggta ttcaggtaga ggtttctgag cctggtcttt ccgatgacgg gaacgttgtt
    18481 cggcgtagcc ggccgcagcc cggtccagtt ctccaccgtg gtcacggctc cgagcgccgg
    18541 gaacagatcg cggatccgcc gcgcgatcgc cgcgcagcgc gcggaattca acgtcgtgtc
    18601 gtagccggtg agctctgcag ttcccgcggc acgcaggcgg ttgccgagcc gcgagatgac
    18661 gatcttgaag gcctcgtcgg tcaggctcac ggtgggcgcc gcgccctgct cggcggggcc
    18721 gagcggcagg gtgatcgaat agcccttcat cggatacacc gggatacgca cgccgagcgg
    18781 tccgagcagc agcgggctgt agctgccgag cgaaacgaca tacgcatccg cggttgcgct
    18841 cggcgcgccc ttcagccgga ttccccttac ggaaccggct tcggcctcga tggcctcgat
    18901 gtcggcgtcg aagcgaaaag ccacgccgcg cgcagccgcg atgcgcgcga gctcctgggt
    18961 gaagcgccac gcgtcgcccg actcgtcgtt cgggttgtag acgccgccga ccaccggatc
    19021 attcgaatat cgcagcgccg gctcgatcgc atggcactcg cttgcggact tgatctgctg
    19081 tcgagtcgcc tctgcgtgcc gcgacatcgc ctccaggtcg cgctggttgg tcgcgaactg
    19141 caggattccg cgctcgagat ggtcgtagcg caggccgagc tcggcccgca gggcgcgcag
    19201 gcattcgcgg ctgtagccgg cgaggccggc aagcgtgcgg ctatggcgct cgaagcgccc
    19261 cggcaagcat tcatataaga accgcgcgcc ccatgcccac tgcgcccagc tcgcgcgcgg
    19321 ccggaaaagc agcggcgcat cttcgcgccc gagccagcgc aggattttcg gcaccacggc
    19381 gggcttcgcc cagggctcgg cgtgcccggc ggcgatctgg ccgccgttgg cgaacgacgt
    19441 ctccagcccc gccgcatcgc gcctttcgat caccgtcacc tcgtggccgg ccgcggcgag
    19501 ataccaggcg ctggcgacgc cgaccacgcc ggcgccgaga acgatgattt tcactggatt
    19561 atgatagccc catgcccctc ttcgccatcg gcgagcggcg cgtccagctg ctcggcgaac
    19621 accattacat cgctcccgac gcttcagtca tagggtctgt tgttcttgaa aacgatgtga
    19681 gcgtctggtt caaggtggtc atacgcgccg agaacgaccg catcacgatc ggcgagggat
    19741 gcaacatcca ggacgcctcg gtgctgcatg tcgatcccgg ctacccgatg acgctcggga
    19801 agcacgtgag cgtgggccat aaggtcatgc tgcacggctg caccatcggc gagggcagcc
    19861 tgatcggcat caacagcgtg atcatgaacg gcgcgcgcat cggccgcggc acgctcattg
    19921 gcgccaatac cctgatcgcc gagggcaagg agattcccga aggggtgctg gtgctcggct
    19981 cgcccggcaa gatcgtgcgc gagctcaagc aggaggaaag ggactacctg ctgaaagtcg
    20041 cccgcggcta cgtcgatcgc tctcgcttct acaaagcgaa cctgaaagaa atcacttagg
    20101 cgccatcctg atggcgccgt cgaggcggat cgtctcggcg ttcagcatct cgacttcgat
    20161 gatcgccttc acgagctggg cgtattccgc cggccggccc aggcgcgagg ggaacggcac
    20221 ctgcttgccg agcgattcct gcgcgtcctt gggcaggccc atcagcatcg gtgtctcgaa
    20281 gatccccggg gcgatggtgc agacgcggat gccgttgcgc gacaggtcgc gcgcgatcgg
    20341 cagcgtcatc ccgaccacgc cgcccttgga agccgagtag gccgcctgcc cgatctgccc
    20401 gtcgaacgcg gcgaccgacg cggtattgac gatcacgccg cgctcgcccg acgcattggg
    20461 caccgccttc gccatcgcat cggcggcgag ccggatcatg ttgaacgtgc cgatgaggtt
    20521 cacgttcagg acgcggatga agcgctgcag gtcgtgcggc gcttccttgc cgagggtgcg
    20581 ttccgcgagc gcgatgccgg cgcaattcac cagcacgtgt agcccgccga attctttcag
    20641 cgcggccgcg accgcggcct tgccttctgc ttccgacgtg acgtccgtct tcacgaagcg
    20701 cccgccgagc tccttggcga gcttctcgcc cgcttcggcc tgcatgtcgg cgatcacgac
    20761 cttgccgccg ttctctgccg ccatgcgagc gctcgccgcg ccgaggccgg aagcgccgcc
    20821 ggtcacgagg aatatgctat ttcggatttg catcggaaac tccgcagtga attcgccgat
    20881 aattgtaacg gcatgcaact cggtttcgac attcgtttca acgatttata cgagcgcgcg
    20941 ggactccttc gcgtcgatgc cgcgtttctt cgttttctcc gcgaggtcga tggcacgctc
    21001 cacgaccggc tgctcgcggc gcgcgagcgc ccgctcgagg gcaagccgga gtccgatctc
    21061 ctcgtagcgc tcgcgccgca cgtcgaagac ttcctcccgc gcctcttcgg catcgaggcc
    21121 gaggcgcgcg cgctggccga acggcacaac gagctcgccc cgctttactc cgtcaagcgc
    21181 ctcttcgtgc agcggcgcgc gctccacaaa gtcaagctgg aggatgcaag accagacggc
    21241 tttcaattca cgaccgagct cgactttgcg cgccaggtca ccgactggct gaaggacgaa
    21301 gccgcgaacg gcgaaaagct ggaacaggcc gcgcggtacg ccgcctgggc cacgctcact
    21361 cccgagggca aggagaagca ccgccacggt gttctgttca aggcgtcgcg caagctcgac
    21421 tacatgaggc tgattccgca caccgagccg cgccagcgcc gggggttcgc cctgaccgac
    21481 cccggcaccg acctggtcgg ggcgctggat caggcgaact actgcatctg gtgccacgag
    21541 cagggaaagg actcctgctc caagggcttc aaggaaaaag cgcctgccga aggattccgg
    21601 aaaaccgtct tcggagtgcc gctcgccggc tgtccgctcg aggaaaagat ctccgagttc
    21661 cacctggtga aggcgcgcgg cgaaccgctc gcggcgctcg gcatcatcgc ggtcgacaac
    21721 ccgatggtcg ccgccaccgg ccaccgcatc tgcaacgact gcatgaagtc ctgcatctac
    21781 cagaagcagg aaccggtcaa catcccgcag gccgagacgc gcacgctcaa ggacgtgctg
    21841 gagttgccgt ggggattcga gatctactcg cttctcacgc gctggaaccc gttcgacctg
    21901 cgccgtcctt atccgaaagc gtcgtcggac aagcgcgtcc tggtcgtcgg tctcggaccc
    21961 gcgggtttca ccgtagcgca ccacctgatg aatgacggcc acacggtggt cggcatcgat
    22021 gggctgaaga tcgaaccgct gga
//
LOCUS       DATBSZ010000263         8516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1143200, whole genome shotgun sequence.
ACCESSION   DATBSZ010000263 DATBSZ010000000
VERSION     DATBSZ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8516
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143200"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VIG70_16930"
     CDS             complement(<1..744)
                     /locus_tag="VIG70_16930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8252281.1"
                     /translation="MPYPIRAAKVVLAVSLAAGCSKEAPPPPRPAPQVTVMTVKPQSI
                     PYIASFVAQTESSRQVDIVARISGFLDKIAYTEGELVEEGKVLFQIDPKPFRAQLDSA
                     MGELKAQEARHSTAAANLKRVKPLAEQRALPQADLDKAQGEFDASRAAVFSAQAKVKE
                     QQLNLGYTTIRTPVTGVASRSLQREGSFINSMADSAKLTYVAALDPMWVNFSVSQNQM
                     AKQREMVEKKQLTLPPDLKFEVEVVLPDGT"
     gene            820..1980
                     /locus_tag="VIG70_16935"
     CDS             820..1980
                     /locus_tag="VIG70_16935"
                     /inference="COORDINATES: protein motif:HMM:NF014577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HEY8252282.1"
                     /translation="MVKIQRGLILAAAFSGLASGQPAAPRLEISGQIMLDAIYDFKRV
                     DPDWNATLRPSTIPVNCPGDAGCGKDGETIMSIRQSKIAFKGFIPTPKGELKTLLDFD
                     LFDVGGGNTHARVLNAWAELGAFGAGQTDTLFMDIDTFPNTIDYWGPSGMVYLRNPQV
                     RYTPVRREGMKVAFSLEAPNSAIDTGKVAVADPALGAAIVGHSRYPDLVGNVRFDRDW
                     GHFQAAAILRSVGFETQGTPNSEPSGAETGYGLNLSGHWNTPGGNRVVGQIVYGRGIA
                     SYMNDGGADLAPDGSFHAEAVKTVGWFVYYDHSWNDQWTSSVGYSEHRQSNTGGQLGT
                     AFKKGSYASANLLHSPVKNVTVGAELLWGQHEQASGATGDDTRLQFSARYAF"
     gene            1981..3033
                     /gene="cax"
                     /locus_tag="VIG70_16940"
     CDS             1981..3033
                     /gene="cax"
                     /locus_tag="VIG70_16940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEY8252283.1"
                     /translation="MNWLFALVPVALVLEHTGAPAPWVFFAAALAIVPVARLIVRSTE
                     QLATYTGDAVGGLLNATFGNAPELIIALVALRAGLLDMVRASIAGAILANLLLALGIA
                     FLLGGLRFHTQTYNVAAARLYSSMMLVAVVSLMVPSSFSRFFAPGETLREEALLNVGL
                     AVVLLAAYALYLVFMLKTHAEEFKAEQGGHESHGPRWSLPRAIGGLIAASLLAAWMSE
                     VLVGAAEATGEALGMSQVFIGIVFLAIVGGAAESSSAIAMARRNKMDLTLGIALGSCI
                     QIALFIAPVLVLASYFVAPQPLELSFNRAEIGALFMAVLIAAVVAGDGRSNWYKGVQL
                     IAVYLIIALMFYFIPA"
     gene            3030..3545
                     /locus_tag="VIG70_16945"
     CDS             3030..3545
                     /locus_tag="VIG70_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252284.1"
                     /translation="MKRVLDPVDRASEIVFGLLMALSFTGSLSVASAGREEVRTMMAA
                     ALGCNLAWGLADAVMYLVGIVVERARAAKLAGAPAARGHRLLGRDDYSGALGVFLMVV
                     LATFPVVIPFAVFDNTAFALRISNLVALATLFAAGWMLGRYAGLSAWRAGAVMAALGA
                     ALVAAIMALGG"
     gene            3573..5231
                     /locus_tag="VIG70_16950"
     CDS             3573..5231
                     /locus_tag="VIG70_16950"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEY8252285.1"
                     /translation="MIAQKLTLKALQVNAGAALPALAVALGYYLAARVGLAFTLQPDP
                     ISTLWPPNALLMAALLLSAPRSWWVLLAAALPAHLLAELQSGVPLAMVLGWFASNCSE
                     ALIGAALVRWCIAAPVRLDSLRNAFILLAFGGLVAPLASSFLDAGLVKLVGFGEGEYW
                     TLVRTRFSANVLAEITIVPLVLAWASWRPAAIRATPLAHSVEVAALFLGLFAVSLLAF
                     DLPLFGASLAPALWYAPLPFLLWAAVRLGTIGTTSALALLVVATIWGAVHGVGPFTGG
                     TPQDTARDLQLFLIAASVPLLLLAVALEERSRAEREAHEQRLQLTHLSRVAMLGEMSG
                     GLAHELNQPLTAILSNAQAAEHLIANKTIDNNELREILRDIIAANQRASEVIRRLRAL
                     FKRGETYRERLDANELVRETLSLAQGDLATRSIAPVLKLAPGTLAIHGDRIQLEQVML
                     NLILNGAEAMAGHTAAERTITVRSAGNDSWVYVSFADRGPGFAPELIEKLFEPFYTTK
                     PQGLGLGLSISRSIVLAHGGRLWGASHAAHGASFHIALPAVRSS"
     gene            5292..5672
                     /locus_tag="VIG70_16955"
     CDS             5292..5672
                     /locus_tag="VIG70_16955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8252286.1"
                     /translation="MPFIAVVDDEESVRKALRRLLRAAGLEAESYASGQEFLAQAAQR
                     EPDCVVLDLHMPSMSGRELLPKIRKLATRPPVVVITAHDTADNEEECLAAGACAYLRK
                     PLEDRLLLNAISAALRVNASGAKR"
     gene            5669..6724
                     /locus_tag="VIG70_16960"
     CDS             5669..6724
                     /locus_tag="VIG70_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003070234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY8252287.1"
                     /translation="MTERWWWVASGVIALSAWTAFAFFLPASAPQIVERDGMMWVPGG
                     SFLMGSDHKLAQDNERPAHRVKVDGFWMDRYHVTNAEFRKFIQATGYVTTAERKPDWE
                     TLRVQSPPGTPKPPDEVLVPGAMVFVGTDAPVSLRDWSQWWRYVPGASWKHPQGPGST
                     IEGKDDHPVVQVSYEDAQAYAKWIGKRLPTEAEWEFAARGGLEQATYAWGDDLAPEGQ
                     QMANYWDVKQGRFPVMRAKVGTSPVGTYPPNGYGLHDMTGNAWQWVADRYGFDHFREQ
                     SKLKLIENPKGPAQSYDPDDGAVPVHAPKRVTRGGSFLCNVDYCLSYRPSARRGTDPY
                     NPMSHIGFRLVKDGNAK"
     gene            6721..>8516
                     /locus_tag="VIG70_16965"
     CDS             6721..>8516
                     /locus_tag="VIG70_16965"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEY8252288.1"
                     /translation="MKRTAIALFTLCCAVPALAQTKAAAPNRSVLPIAEPVYQPITEL
                     DARKAKAPARFQVKAPAGAPNVVIVLIDDIGFGHSSAFGGPIRMPTLEKMAANGLKYN
                     RFHTTALCSPTRTALLTGHNHHANNAGAIMEVATAFPGNTGIRPRSITTLAEILRQNG
                     YSTSAFGKYHETPPWEVSVSGPYDRWPTGSGFDKFYGFIGGETNQWAPAIYDGVTRVE
                     HRQTPDYHFTVDMTDQAINWVSAQQALTPGKPFYMYFATGATHAPHHAPKEWIAKYKG
                     EFSGGWDRLREETFARQKKLGVVPQNAKLTPRPKEIPAWADMSADQKRLFERQMETFA
                     GFAEHTDNEVGRLVAQLEAIGALENTLFYYIVGDNGASAEGGPEGAYNEMMALNGIIG
                     KADQMMPHIDDWGGPTTFPHFAIGWAWAGNTPFQWTKQVASHFGGTRNGMVLHWPKGI
                     KAKGEVRSQFHHVVDVAPTALAAAKLPQPKVVNGVKQRPMDGVSMLYSANNAKAADRR
                     KTQYFEMFGNRGIYHDGWVACTRHSIPWDMAAKMRPLADDVWELYNVNEDFSQANDLA
                     SKHPEKLKELQAVFMKEAVRNHVLPIDDRRAER"
BASE COUNT         1357 a         2945 c         2857 g         1357 t
ORIGIN      
        1 cgtgccgtcg ggcagcacga cctcgacctc gaacttgagg tcgggcggca gcgtgagctg
       61 cttcttctct accatctcgc gctgcttcgc catctggttc tgcgacacgc tgaagttcac
      121 ccacatcggg tcgagcgccg ccacgtaggt gagcttcgcg ctgtcggcca tcgagttgat
      181 gaagctgcct tcgcgctgca gcgagcggct cgccacgccg gtcaccggcg tgcggatcgt
      241 cgtgtagccg aggttgagct gttgctcctt gaccttcgcc tgcgccgaga acaccgcggc
      301 gcgcgacgcg tcgaactcgc cctgcgcctt gtcgaggtcg gcctgcggca gggcgcgctg
      361 ctcggcgagc ggcttgacgc gcttgaggtt ggccgccgcg gtggagtggc gcgcctcctg
      421 cgccttgagc tcgcccatgg ccgagtcgag ctgcgcgcgg aacggcttcg ggtcgatctg
      481 gaacagcacc ttgccctcct cgaccagctc gccctcggtg taggcgatct tgtcgaggaa
      541 gcccgagatc cgggcgacga tgtccacctg gcgcgagctc tcggtctgcg ccacgaagct
      601 ggcgatgtac gggatcgact gcggcttcac ggtcatgacc gtgacctgcg gcgccggccg
      661 cggcggcggc ggggcttcct tggagcagcc cgccgcgagc gagacagcca gaacgacctt
      721 cgcagcgcga atcgggtacg gcatgggcac cttttctttc tcccctaagt tctcacacac
      781 catcttgcac gcctatagga ctaaaggcca atatcgcgca tggtgaaaat acagcgcggg
      841 ctcatcctgg ccgcggcctt cagcggactc gcctccggac agcctgcggc cccccgcctg
      901 gaaatttccg gccagatcat gctcgacgcg atctatgact tcaaacgcgt cgatccggac
      961 tggaacgcca cgctgcggcc ctccacgatc ccggtgaact gcccgggcga cgcgggctgc
     1021 ggcaaggacg gcgagaccat catgagcatc cgccagtcga aaatcgcgtt caaaggattc
     1081 atcccgacgc cgaagggcga gctcaagacg ctgctggatt tcgatctttt cgacgtcggc
     1141 ggcggcaaca cgcacgcgcg cgtgctcaac gcgtgggcag agctgggcgc gttcggcgcg
     1201 gggcagaccg acacgctctt catggatatc gacaccttcc cgaacaccat cgattactgg
     1261 gggccgagcg gcatggtgta cctgcgcaat ccgcaggtgc gctacacgcc cgtgcgccgc
     1321 gaagggatga aggtcgcgtt ttcgctcgag gcgcccaact cggcgatcga caccggcaag
     1381 gtcgcggtcg ccgatccggc gctcggcgcg gcgatcgtgg gccacagccg ctatccggac
     1441 ctggtcggca acgtgcgctt cgaccgcgac tggggtcatt tccaggccgc cgcgatcctg
     1501 cgcagcgtcg gcttcgagac ccagggcacg cccaattctg agccttcggg cgcggagacc
     1561 ggatacggcc tgaacctgag cggccactgg aacacgcccg gcggcaaccg ggtggtcggg
     1621 cagatcgtct acggccgcgg catcgcgagc tacatgaacg acggcggcgc cgacctcgcc
     1681 cccgatggct ccttccatgc cgaggcggtc aagaccgtgg gctggttcgt ctactatgac
     1741 cacagctgga acgaccagtg gacgagctcc gtgggctaca gcgagcatcg ccagagcaac
     1801 accggcggac agctcggcac ggcgttcaag aaaggcagct atgcctctgc gaacctgctg
     1861 cattcgccag tcaagaacgt caccgtgggc gcagagctcc tgtggggcca gcacgaacaa
     1921 gcgagcggcg cgaccggtga cgacacacgc ctccagttct cggcccggta cgccttctag
     1981 atgaactggc tgttcgccct ggttccggtc gcgctcgtcc tcgagcacac cggcgcgccg
     2041 gcgccgtggg tgttcttcgc cgcggcgctc gccatcgtgc cggtcgcgcg cctcatcgtg
     2101 cgctcgaccg agcagctcgc gacctacacc ggcgacgcgg tcggcggact gctcaacgcg
     2161 actttcggca acgcgcccga gctgatcatt gcgctggtgg cgctgcgcgc cggcctgctc
     2221 gacatggtgc gcgcctcgat cgccggcgcg atcctcgcca acctgctgct cgccctcggc
     2281 atcgcgttcc tgctcggcgg cctgcgcttc cacacgcaga cctacaacgt ggccgcggcg
     2341 cggctctaca gctcgatgat gctggtcgcg gtggtcagcc tgatggtgcc gagctccttc
     2401 agccgcttct tcgcgcccgg cgagaccctg cgcgaggaag cgctgctcaa cgtcggtctc
     2461 gccgtggtgc tgctcgccgc ctatgcgctg tatctcgtgt tcatgctgaa gactcacgcc
     2521 gaggagttca aggccgaaca aggcgggcac gaaagccatg gcccgcgctg gagcctgccg
     2581 cgggcgatcg gcgggctcat cgccgcgtcg ctgcttgcgg cgtggatgag cgaggtcctg
     2641 gtgggcgcag ccgaggccac cggggaggcg ctcggcatgt cgcaggtgtt catcggcatc
     2701 gtgttcctcg ctatcgtcgg cggcgcggcc gagtcgagct cggcgatcgc gatggcgcgg
     2761 cggaacaaga tggacctgac gctcggcatc gcgctcggca gctgcatcca gatcgcgctc
     2821 ttcatcgccc cggtgctggt gctggcgagc tacttcgtgg cgccgcagcc gctcgagctt
     2881 tccttcaacc gcgcggagat cggcgccctc ttcatggcgg tgctgatcgc cgcggtggtg
     2941 gccggcgacg gccgctcgaa ctggtacaag ggcgtgcagc tcatcgccgt ctacctgatc
     3001 atcgcgctca tgttttattt cattcccgca tgaagcgggt cctggacccg gtcgaccgcg
     3061 cctccgagat cgtcttcggc ctgctgatgg cgctgtcctt caccggctcg ctcagcgtgg
     3121 cgagcgccgg gcgcgaggag gtgcgcacca tgatggccgc ggcgctcggc tgcaacctcg
     3181 cctgggggct ggccgatgcg gtgatgtacc tggtcggcat agtcgtcgag cgcgcgcgcg
     3241 cggcgaagct ggccggcgcc cccgccgctc gcgggcatcg gctgctgggg cgcgacgact
     3301 actcgggcgc gctcggcgtg ttccttatgg tggtgctggc gacctttccg gtggtgattc
     3361 cgttcgccgt gttcgacaac accgcgttcg cgctgcggat ctcgaacctg gtcgcgctcg
     3421 ccacgctgtt cgcagccggc tggatgctcg ggcgctacgc gggcctcagc gcctggcgcg
     3481 cgggcgccgt catggccgcc cttggcgccg cgctggtcgc cgcgatcatg gccctgggcg
     3541 gctagcgagc gaagaccggc taaactagtg cgttgattgc gcaaaagttg acgctgaagg
     3601 ccctgcaggt caacgccggg gctgccctcc ccgcgttggc cgtcgcgctc ggctattacc
     3661 tcgcggcgcg cgtcggctta gccttcaccc ttcagccgga tccgatctcg acgctgtggc
     3721 cgccgaacgc gctgctgatg gcggcgctgc tgctttcggc gccgcgctcg tggtgggtgc
     3781 tgctcgccgc ggccctgccc gcgcatctgc tggccgagct gcagagcggc gtgccgctcg
     3841 cgatggtgct cggctggttc gcgagcaatt gcagcgaggc gctgatcggc gccgcgctgg
     3901 tgcgctggtg catcgccgcg ccggttcggc tcgactcgct gcgcaacgcc ttcatcctgc
     3961 tcgccttcgg tggcctcgtg gcgcccctcg cctcctcgtt cctcgacgcc ggcctcgtga
     4021 agctcgtcgg cttcggcgag ggcgaatact ggacgctggt gcgcacgcgc ttttccgcca
     4081 acgtgctcgc cgagatcacg atcgtgccgc tggtgctcgc ctgggcgagc tggcgccctg
     4141 cggccatccg cgctacgccc ctcgcgcaca gcgtcgaggt ggcggcgctg ttcctggggc
     4201 tgttcgcggt cagcctgctc gctttcgacc tgccgctctt cggcgccagc ctggcgcctg
     4261 cgctttggta cgcgccgctg ccattcctgc tctgggccgc ggtgcgcctc gggacgatcg
     4321 gcaccacgag cgcgctcgcg ctgctggttg tcgccacaat ctggggcgcg gtccacggcg
     4381 tcgggccgtt caccggcggc acgccgcagg acaccgcgcg cgacctgcag ctcttcctga
     4441 tcgcggcctc ggtgccgctc ctgctgctcg cggtggcgct cgaggagcgc tcgcgcgcag
     4501 agcgcgaggc gcacgagcag cgcctgcagc tcacgcacct ctcgcgcgtg gcgatgctcg
     4561 gcgagatgtc gggcggcctc gcgcacgagc tgaaccagcc gctgaccgcc atcctctcca
     4621 acgcgcaggc ggccgagcac ctgatcgcga acaagacgat cgacaacaac gagctgcgcg
     4681 agatcctgcg cgacatcatc gccgcgaacc agcgcgccag cgaggtgatc cggcgcctgc
     4741 gcgcgctgtt caagcgcggc gagacctatc gcgagcggct ggacgcgaac gagctggtgc
     4801 gcgagacgct gagcctcgcg cagggcgacc tcgcgacccg cagcatcgcg cccgtgctga
     4861 agctcgcccc ggggacgctc gcgatccacg gcgaccgcat ccagctcgag caggtgatgt
     4921 tgaacctcat cctgaacggc gccgaggcca tggcaggcca cacggcggcc gagcgcacga
     4981 tcaccgttcg ctcggcgggc aacgattcct gggtgtacgt gagcttcgcc gaccgcggcc
     5041 cgggcttcgc tcccgagctg atcgaaaagc tgttcgagcc gttctacacc accaagccgc
     5101 agggcctggg gctgggcctc tcgatctcgc gctcgatcgt gctcgcccac ggcggccggc
     5161 tgtggggcgc gagccatgcg gcgcacggcg cgagcttcca catcgctttg cccgccgtcc
     5221 ggtcaagctg agcgcatggg accttggtcc aattacgctt tcatgcgcgg cctgcttgaa
     5281 tcgtctccgc catgcccttc atcgcagtcg tggatgacga ggagtcggtc cgcaaggcct
     5341 taagacgcct gctgcgcgcc gccggcctcg aggccgaaag ctacgccagc ggacaggagt
     5401 tcctcgcgca ggcggcgcag cgcgagcccg actgcgtggt gctcgacctc cacatgccga
     5461 gcatgagcgg ccgcgagctc ctgccgaaga tccgcaagct cgccactcgc ccgccggtgg
     5521 tcgtcatcac cgcgcacgac acggccgaca acgaggagga gtgccttgcc gccggcgcct
     5581 gcgcctacct gcgcaagccg ctcgaggacc ggctgctcct caacgcgatc tcggccgcgc
     5641 tgcgggtaaa cgcaagcggc gccaaacggt gacggaacgt tggtggtggg tcgcctcggg
     5701 ggtcattgcg ctctccgcgt ggacggcgtt cgcctttttt ctccctgcca gcgctccgca
     5761 gatcgtggaa cgcgacggga tgatgtgggt tccgggcggc tctttcctca tgggcagcga
     5821 ccacaagctc gcgcaggaca acgagcggcc agcacaccgc gtcaaggtcg acgggttctg
     5881 gatggaccgc taccacgtca ccaacgcgga attccgcaag ttcatccagg ccaccggcta
     5941 cgtcaccacc gccgagagga agcccgactg ggaaacgctc agggtgcagt cgccccccgg
     6001 cacgccgaag ccgcccgacg aagtgctcgt gcccggcgcc atggtgttcg tcggcaccga
     6061 cgccccggtc tcgcttaggg attggtcgca gtggtggcgc tacgtcccgg gcgcgagctg
     6121 gaagcatccg cagggccccg gcagcacgat cgagggcaag gacgatcatc cggtggtgca
     6181 ggtgtcgtac gaggacgcgc aggcgtatgc gaaatggatc ggcaagcgcc tgccgaccga
     6241 ggcggaatgg gaattcgccg cgcgcggcgg gctggagcag gcgacctacg cctggggcga
     6301 cgacctcgcg cccgagggcc agcagatggc gaactactgg gatgtgaagc agggccgctt
     6361 cccggtgatg cgggcaaagg tcggaacctc gccggtgggc acgtatccgc cgaacggtta
     6421 cggtctgcac gacatgaccg gcaacgcctg gcaatgggtc gccgaccggt acggcttcga
     6481 tcatttccgc gagcagtcaa aactgaaact gattgaaaac cccaaaggcc cggcgcagag
     6541 ctacgacccc gacgacggcg ccgtgccggt acacgctccg aagcgcgtga cgcgcggcgg
     6601 ctctttcctg tgcaacgtcg actactgcct gtcgtaccgc ccgagcgcgc ggcgcggcac
     6661 cgatccctac aacccgatgt cgcacatcgg ctttcgactc gtgaaggacg ggaacgcaaa
     6721 atgaaacgca ccgcaatcgc attattcacc ttgtgctgcg cggttccggc gctggcccag
     6781 accaaggccg cggcgccgaa ccggtccgtg ctgccgatcg cggagcccgt ttaccagccg
     6841 atcaccgagc tggacgcacg taaggcgaag gcgccggcac gcttccaggt gaaggccccg
     6901 gcgggcgcgc cgaacgtggt gatcgtgctg atcgacgaca tcggcttcgg ccacagcagc
     6961 gccttcggcg ggccgatccg catgcccacg ctggagaaga tggcggccaa cggtctcaag
     7021 tacaaccgct tccacaccac cgcgctctgc agccccaccc gcacggcgct actgaccggg
     7081 cacaaccacc acgccaacaa cgccggcgcg atcatggagg tggccaccgc attcccgggc
     7141 aataccggca tacggccgcg cagcatcacg acgctcgccg agatcctgcg ccagaacggc
     7201 tacagcacct ccgcgttcgg caagtaccac gagacaccgc cctgggaagt ctcggtctcc
     7261 ggtccttatg accgctggcc caccggctcg ggcttcgaca agttctacgg cttcatcggc
     7321 ggcgagacca accagtgggc gcccgccatc tacgatggag tgacgcgcgt cgagcacagg
     7381 caaacccccg actaccactt caccgtggac atgaccgacc aggcgatcaa ttgggtcagc
     7441 gcgcagcagg ccctgacgcc gggcaaaccg ttctatatgt acttcgccac cggcgcgacg
     7501 cacgcgccgc accatgcgcc aaaggagtgg atcgccaaat acaaggggga attcagcggc
     7561 ggctgggatc ggctgcgcga agagaccttc gcgcgccaga agaagctggg cgtggtcccg
     7621 caaaacgcga agctcacccc gcggccgaag gaaattcccg cctgggcgga catgagcgcc
     7681 gaccagaagc gcctgttcga gcggcagatg gagaccttcg cgggctttgc cgagcacacc
     7741 gacaacgaag tggggcggct ggtggcgcaa ctcgaggcga tcggcgcgct ggagaacacg
     7801 ctcttctatt acatcgtggg cgacaacggc gccagcgccg aaggcgggcc ggagggcgcc
     7861 tataacgaga tgatggcgct caacggcatc atcggcaaag ccgaccagat gatgccgcac
     7921 atcgatgact ggggcggccc gaccacgttc ccgcacttcg ccatcggctg ggcctgggcg
     7981 ggcaacacgc ccttccagtg gaccaagcag gtggcgagcc atttcggcgg cacgcgcaac
     8041 ggcatggtgc tgcactggcc gaaaggcatc aaggccaagg gcgaggtgag gagccagttc
     8101 caccacgtgg tcgacgtcgc gccgacggcg ctggcggccg cgaagctgcc gcagcccaag
     8161 gtggtgaacg gcgtgaagca acggccgatg gacggcgtct cgatgctgta ctcggcgaac
     8221 aacgccaagg ccgccgaccg gcgcaagacc cagtacttcg agatgttcgg caacaggggc
     8281 atctaccacg acggctgggt ggcgtgcacg cggcattcca tcccctggga catggccgcg
     8341 aagatgcgcc cgctggcgga cgacgtgtgg gagctctaca acgtgaacga ggatttcagc
     8401 caggcgaacg acctcgcaag caagcacccg gagaagctga aggagctgca ggcggtcttc
     8461 atgaaggagg cggtgcggaa ccacgtgctg ccgatcgacg accggcgcgc cgagcg
//
LOCUS       DATBSZ010000264        11924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2100464, whole genome shotgun sequence.
ACCESSION   DATBSZ010000264 DATBSZ010000000
VERSION     DATBSZ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2100464"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..629)
                     /locus_tag="VIG70_16970"
                     /pseudo
     CDS             complement(1..629)
                     /locus_tag="VIG70_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
     gene            complement(654..1628)
                     /locus_tag="VIG70_16975"
                     /pseudo
     CDS             complement(654..1628)
                     /locus_tag="VIG70_16975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1914..3098
                     /locus_tag="VIG70_16980"
     CDS             1914..3098
                     /locus_tag="VIG70_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8252289.1"
                     /translation="MNSTKRNVGLLAACQALLFTNNSTLIAINGLAGLALAPYVGLAT
                     LPVTCWVLGGAIGTMPASLHMKRVGRQRGLRSGTFWGIAGALICAAAIWAQSFWMLCL
                     GTLVWGVYNAYGQYYRFAAADVASPDFRPTAISLVLAGGLVGGVLGPTSSRWTIELLA
                     PKFMGAYLALIGFALLTMLLLRFIRIPTPSAAEQSATGRPLREIAAQPKFIVAVLAGA
                     IGYGVMNFLMTSTPIAMQICGHAYGDAAFVISSHVIGMFAPSFVTGPLIKRIGALPVM
                     FAGVLLNFVAIGIALSGIAVAQFWWSLVILGVGWNFLYISGTTLLTTTYRPEERARAQ
                     GTNEQAIFIMMAISSLSSGLTVTAGGWERVNLLALPAVATIGIALIWFALRQRAEKAA
                     AA"
     gene            3095..4189
                     /locus_tag="VIG70_16985"
     CDS             3095..4189
                     /locus_tag="VIG70_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEY8252290.1"
                     /translation="MSQPAPGKIGQRLEDVDTPALILDLPSFEKNQKTLFDLVKNRVR
                     VRPHAKTHKCPEVAKRQMALGAVGMCCQKVSEAEAMVDGGIGDVLVSNEIVGAPKLER
                     LAALSRRAKIGVCVDHVDNLRAIAATGAKLDVYIEIDVGMRRCGVAPGEPAVALAREI
                     VTHTHLRFAGLQAYHGRAQHIRSMEERHAVIETAAQHVQHTKRLLKQAGIACPVVTGA
                     GSGTFMLEVEAGAWDEIQPGSYAFMDADYAKNEWAAPLPRFEHALFVLSTVMSRPSAS
                     MAIVDAGLKASSVDSGMPAVWQRPGLAYTHASDEHGWVEGKPAPALGEKLLLVPGHCD
                     PTINLYDWYVVVKSGFVVDLWPISARGAVT"
     gene            4877..5275
                     /locus_tag="VIG70_16990"
     CDS             4877..5275
                     /locus_tag="VIG70_16990"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8252291.1"
                     /translation="MKKQYLNPKEMSTPRFYSHAVTAEGTGKRVYVSGQVSWDAAGNV
                     VGKGDMRAQSEQVFKNVAAALKAAGAGWGDVIKMNGYMVGMNAERVGAYREVRQRFLK
                     EGALPASTLVGVEALVHPDLLLEVEVIAAI"
     gene            5272..6450
                     /locus_tag="VIG70_16995"
     CDS             5272..6450
                     /locus_tag="VIG70_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8252292.1"
                     /translation="MSQGNYGWVVVAAAFTLMLVGFSAAYSFAAFFTAFEAEFGASRG
                     DIALVFSVAAFLWFLLGAPAGVLADRYGVRKVALVGVACLALALWLASRAQSLAMLYA
                     TYSIGIGLGVGLTYVPSIGAVQPWFAANRAFASGLAVAGIGAGNIAGPLLAAWWIGLF
                     GWRGAYVALAVLVLVLGSVAALAIRETEHRALGTREGVTLAEAAKTTPFWLLFASLVL
                     SCIGLFVPMVHLGPYAVDAGYTQAQGVTLVSLIGLGSLLGRFTIGVPADRLGRMPSLS
                     AMYAGLGAMFMLWWAAEAYWVLALFALVFGICYGAYVALLPTIVMDLYGPRSVSGILG
                     CLYTGCGVGTLVGPWLAGVAFDARGSYQLPMLAGAACSLAAAACILPLHGKAKALYRS
                     "
     gene            6464..7174
                     /locus_tag="VIG70_17000"
     CDS             6464..7174
                     /locus_tag="VIG70_17000"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEY8252293.1"
                     /translation="MRTLHVGGEAIQSSMRLEDPYALALDYTRCMMAFLLFHPEPREA
                     LMIGLGGGSLAKFFFRHLRRTRVRIVELDSRIVTAAREHFALPPDGERLRVEVGDGAE
                     ALTPECCDVLVIDAFEDELHVPRLASGEFYDGAFLALNERGALVVNFMNDDPKLDATL
                     ERLERAFGGAVICMPALYDPNILAFAFKGVSPAISWSALRSRAEQLESRLGLPFTRYV
                     SRLRAMNRWNAAELIIAA"
     gene            7171..7800
                     /locus_tag="VIG70_17005"
     CDS             7171..7800
                     /locus_tag="VIG70_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY8252294.1"
                     /translation="MKFTSESFADGGAMPEHCAFCVPDAASRAKLGQNRNPQLAWSDL
                     PKGTQSVVIICHDPDVPSKADDVNQEGRTIPASLPRVAFTHWVLIDMPASGSPIRDGE
                     FSSGVTPKGKSGPLGARNTRQGLNDYTVWFRNDPNMAGDYFGYDGPCPPWNDSIPHHY
                     VFTLFALDVEKCPVQRKFTSAHVLKAMKGHVLGTAKITGRYSLNPAVTV"
     gene            7797..8525
                     /locus_tag="VIG70_17010"
     CDS             7797..8525
                     /locus_tag="VIG70_17010"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8252295.1"
                     /translation="MIEAAALAAIAFVGALVFGVTGFGAALVTIPLATHFVPLKFALA
                     LFVLADLAAAISVGLENPRKAVRSEWTRLVPMIFAGTALGVTLLVNLPRAAGMVLLGL
                     FILWFAILNLVRRASMKIVSPRWAWVAGFAGGITSTVFGAGGPPYAIYLSQRGLTKEQ
                     FRATLGFATLTSISLRLIAFAITGLLLDREVWLKALAVVPACLFGIWVARKIFRRISR
                     DALMRVVALMLLASGTSLIFRAVL"
     gene            complement(8503..8838)
                     /locus_tag="VIG70_17015"
     CDS             complement(8503..8838)
                     /locus_tag="VIG70_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8252296.1"
                     /translation="MTIECPVLDVYNFIVEPRNLPKWASGLASGPGGAPAKVRFVERN
                     KWGVLDHYVTVGSGPEVYMPMRVFQNGDGAELLITVFRQPGVTEEKFVDDTQWVRRDL
                     EALKELLEK"
     gene            complement(8865..10382)
                     /locus_tag="VIG70_17020"
     CDS             complement(8865..10382)
                     /locus_tag="VIG70_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8252297.1"
                     /translation="MRRHQSIWAATAALPSFTPLEQNIHVDVCIVGAGISGLTTAYVL
                     ARAGKRVAVLDDGPVAGGMTQMTTAHITNQLDDRYFELEKLHGREGARLAADSHTAAI
                     DRIETIVRQESIDCDFARVDGYLFLAEGDTRGTLEHELEAAHRAGLRAVELLERAPFR
                     SFDPGPCLRFPAQGQFHPLKYLSALAEAIHRAGSRIFTGTHADQIEGGVPALVHAGKH
                     VVTADAVVVATNVPVNDRVAIHTKQAPYMTYVVAARVPQGSVPTVLSWDTGDPYHYLR
                     LYDDLLIVGGEDHKTGQASDTPERYARLEAWARARFPEMGEVELAWGGQVMETQDFLA
                     FIGRNPMDHENVYVATGDSGMGITHGTIAGMLLSDLILGLTNPWEALYDPSRVTLRAV
                     GDFARENSNVALQYTDWLTGGDVASADDVRPGSGAIVRRGLEKIAVYRDEQGEVHECL
                     AKCPHLGCVVHWNPAEITWDCPCHGSRFDRYGKVISGPANCDLATAERSREQRAA"
     gene            complement(10411..11769)
                     /gene="gorA"
                     /locus_tag="VIG70_17025"
     CDS             complement(10411..11769)
                     /gene="gorA"
                     /locus_tag="VIG70_17025"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEY8252298.1"
                     /translation="MGFDYDLFTIGGGSGGVRASRVCAAYGAKVAIAEPGRFGGTCVN
                     VGCIPKKLFSYAAHWREDFKLAEAYGWTVGSSSHPLRFDWQTLLKNKDREIARLNGVY
                     ERVLVNAGVEIHRVRATVLGPNEVEIDGKKRTAKHILVATGSWPQLPVIPGIEHAITS
                     NEAFALERLPKRAVVVGGGYIALEFASIFQGLGVETMLTYRGKRLLRGFDAELGTRIA
                     QEMSAKGVNVCFGCEPAAIRKRNDLEIEFTDGRIERTDLVMFATGRRPNTAKLGLESA
                     GVKLAADGAIIVDRFSRTNVDSIHAVGDVTNRINLTPVATAEAMWLARTLFRGEPTPV
                     DHENVPTAVFANPNLATVGLSEERARERFGAVDIYKASFRALKVVMSDKPDTTFMKLV
                     VDRASQRVVGAHMIGADAGEIIQGVAIAVKLGATKAQFDATIGIHPTAAEEFVTMRER
                     AT"
BASE COUNT         1830 a         3889 c         4010 g         2195 t
ORIGIN      
        1 acgatgctca tcacgtgctc ggcgcgctgt gggtaatgct cggcgagcca ttccttgaac
       61 agcgttttca gctcgtgcgg cagccgcatg atgacgtacg cggcttcggc tgcgcccgcc
      121 gcggcggcgg cctcgaggac atgctccatg gtcttatcgt tgagcgccgg gatcaacgcc
      181 gcgaccatga ccccgcaagg gactttgttc tcgctgagtt tcttcaagac gtccagccgg
      241 cgctgcggac tcgccgcgcg cggttcgagc ttgcgcgcga gctcgcggtc gagcgtgccg
      301 atggaaatga acactttcac gagattcttg gccgccatgg gcgcgagcag atcgaggtcc
      361 cgctccacca gcgcggactt gctgacgatg gtcacggggt gctcgcattc cgacaggact
      421 tccaggatct gccgggtaat gcggtgcttg cgctcgatcg gctggtagca gtcggtattg
      481 gcgccgagcg cgatcgggct cgggcgatag cccggcttcg ccagttcctc tttcagcaat
      541 tctgcagcgt tggtcttggc gaacagcttg gtctcgaaat cgagcccggg tgaaagctcc
      601 aggtaggcgt ggctcgggcg cgcgtagcag ttgtggctca ccacgccgtt ggcgatgaaa
      661 tcctcggttt ccgtggtgat gtcgtacagc cgcatcgctt tgccaagcgg ctcgaccctt
      721 accaccccga gccgggcttc gcttttgacc gcttgaccgg cgatgtcacg cttgcggact
      781 atcgcgggat ccacggaatg gaagaagcgc aggtgttcgt gtagtccgcc gagcagtcgg
      841 actacgtcga tcggcttgcg tgtttcatag ggcacgttct gaaccacaaa gcggaaatcc
      901 agcttggtca agctgcggcc gacccatccg atgatgtcct tgtcggtatt ggagattctc
      961 agaataccct cgctataact gccctcggca tcgaagattc cggcaaggaa gcctgcgcac
     1021 cacgcaggcg ccggcgtggt cggccaagcg gtgagcgcgc ggatctgctc cacgccgggt
     1081 cgcgcatggg ttcggatggc gtgcagcagg cggcgtccgg cactcgccgc ctggaatatg
     1141 aaatgttgcg tgtcgacccg gtcccggcgc agatagtcct gcgaccgcag cagcgcttcc
     1201 acgtcgcaca tggcgagacg gaactggtgc tgctgaccgc tttcatagcg gtaagaggcg
     1261 agcaagccgt cgccgcggat cagcccgcac agatacccga gacggtaatc gtggttcttg
     1321 acggccggcg tggcgaagcc gccggtgccc atgagcttgt tgccggttgt gaggtgcggg
     1381 cgccgtgcat cgccttgcat ttttcccgtc acgaatttcc agccgcgttc cgtcaggaaa
     1441 cgatggtcgg ggccggcgat gagagtggtg ccgtcttcaa gcacgatccg ataggccggc
     1501 ttgaccacgc tccagtgtgc gaggactcgg gatttgacgt accggcgata ccagccttcg
     1561 cgtttcgtgc cgtacaacac gtcgccagtg cgcacttcgg cgatgctgcg agtggtaccg
     1621 tctcccatga gcaatggcgt atcgccatgg aggcaataga tgcatccatg ctcgcatcct
     1681 cgataggggt tgatggattg ctcgaacccg acgtcgggcg agtcgttgcg cgagatgatc
     1741 gagcgcgcgc gctcgatcgc gacgctggtc ttcagctcgc gttgatcttg ttcctccggg
     1801 gtccagccgt cgtcgaacgc ctcgcgcgcg acgctttcga aacggccctg cacgaaagag
     1861 cgcgctcccc gaagatagcg ggggtcttgc ttgtccaccg cgatactgta tatatgaaca
     1921 gtaccaagcg caacgtcggt ttgctggccg catgccaggc gctgctgttc acgaacaact
     1981 cgaccctcat agcgatcaac ggcttggcgg gcttggcgct cgccccgtat gtcggcctcg
     2041 ccacgctgcc ggtcacctgc tgggtgctgg gcggcgcgat cggcaccatg ccggcgtcgc
     2101 tgcacatgaa gcgcgtgggc cgccagcgcg ggctgaggag cgggacgttc tggggaatcg
     2161 ccggcgcgct gatctgcgcc gcggcgatct gggcgcagag cttctggatg ctttgcctcg
     2221 gcaccctggt ctggggagtc tataacgcct acggccagta ctaccgcttc gccgccgccg
     2281 atgtcgcgag ccccgatttc aggcccacgg cgatctcgct ggtgctcgcc ggcggcctgg
     2341 tgggcggcgt gctcggcccc acaagcagcc gctggaccat cgagcttctc gccccgaagt
     2401 tcatgggcgc gtatctcgcg ctgatcggct tcgctctgct gacgatgctg ttgcttcgct
     2461 tcatccgcat ccccaccccg agcgccgccg agcagtcggc gaccgggcgg ccgctgcgcg
     2521 agatcgcggc gcagccgaag ttcatcgtcg cggtgctcgc cggggccatc ggctacgggg
     2581 tgatgaactt cctcatgacg tcgaccccga tcgcgatgca gatctgcggc catgcgtacg
     2641 gcgatgccgc gttcgtgatc tcctcgcacg tcatcggcat gttcgcaccg tccttcgtca
     2701 ccggccccct catcaagcgc atcggcgcgc tgccggtcat gttcgccggc gtgctgctca
     2761 atttcgtcgc catcggcatc gcactctccg ggatcgcggt ggcgcagttc tggtggtcgc
     2821 tggtgatcct gggggtgggc tggaacttcc tctacatcag cggcaccacg cttctcacca
     2881 cgacctaccg ccccgaagag cgcgcgcggg cgcagggcac gaacgagcag gccatcttca
     2941 tcatgatggc gatctcgtcc ctcagctcgg gcctgaccgt gaccgccggc gggtgggagc
     3001 gcgtcaacct gctagccctg ccggccgtcg ccacgatcgg catcgcgctc atctggttcg
     3061 cgctgcgcca gcgcgccgag aaggcggccg cggcatgagc cagccggcgc cgggaaagat
     3121 cggccagcgg ctggaggacg tggacacgcc cgcgctgatt cttgatctgc cgagctttga
     3181 aaaaaaccag aagaccctgt tcgacctggt gaagaaccgc gtccgggtgc gcccgcacgc
     3241 aaagacgcac aagtgccccg aggtcgcgaa gcgccagatg gccctgggcg cggtcgggat
     3301 gtgctgccag aaagtctcgg aagccgaggc gatggtcgac ggcggcattg gcgacgtcct
     3361 cgtcagcaac gaaatcgtcg gtgcgccgaa gctcgagcgc ctcgccgcgc tctcgcgccg
     3421 ggcgaagatc ggggtttgcg ttgatcacgt cgacaacctg cgcgcgatcg ccgcgacggg
     3481 agcaaagctc gatgtctata tcgagatcga cgtcgggatg cgtcgttgcg gcgtcgcccc
     3541 gggcgagccc gcggtggcgc tcgcaagaga aatcgtgaca cacacccatc tgcgcttcgc
     3601 gggcctgcag gcctaccacg ggcgcgcgca gcacatccgc agcatggaag agcgccatgc
     3661 ggtgatcgaa accgccgccc agcatgtcca gcacaccaag agattgctga agcaggcggg
     3721 catagcctgc ccggtggtga ccggcgccgg aagcggaact ttcatgctcg aggtcgaggc
     3781 cggcgcgtgg gacgaaatcc agcccggctc gtatgcgttc atggacgcgg actacgcgaa
     3841 gaacgagtgg gcggcgccgc tgccgcgctt cgagcatgcg cttttcgtgc tgagcacggt
     3901 gatgagccgt ccttcggcct ccatggcaat cgtcgacgcc ggcctcaagg cttccagcgt
     3961 ggattcgggc atgccggctg tgtggcagcg gccagggctc gcctatacgc acgcttccga
     4021 cgagcacggc tgggtggagg ggaaacctgc tcctgcgctc ggcgagaagc tgctgctggt
     4081 gccggggcac tgcgacccga ccatcaatct ctacgattgg tatgttgtcg tgaagtctgg
     4141 cttcgtagtg gacctctggc ccatcagtgc cagaggggct gtgacttaga tccgccgtag
     4201 actctgcctg cgatgcgttg gctaacgatg atcctcctgg ctctgagtca tgccgctcta
     4261 ggtgaaaccc tttcgggcac ggtcgttggc gtggtggacg gcgatacgct gacggtcctg
     4321 gattcgcaac agcgacagca cagagtgagg cttgcggaga tcgatgctcc tgagaggcga
     4381 caagcgttct acgtgaggtc tgggaaatcg ctctatggcc tctgcttcaa gaaagccgcc
     4441 caggtcgaat ggcgaaccaa ggaccaaagc aaccgctatg tcggtcaagt cacttgcgcc
     4501 ggtgttgacg ccaacgcaga gcaagtgcgc cgaggcatgg cctgggtttc ccccagcctc
     4561 acccaacctg gctcgctgct ctacgagctg gaggcacacg ccagggtacg aggcattggg
     4621 ctttgggcgg accctcgccc gataccgccg tgggactggc gcccgcggaa gccgacgcca
     4681 tgacgcagca gcgaaaaagg tcggcccgaa gcccgcgggg gatgggctta ccagccaccc
     4741 tgaccgctgg cttcgggcct tcctcctcct cacctaaccc ttcactccca gactcgcaat
     4801 ccgcgtggat tcaggttgca cgcgttgcca gaggacgcgc aatagcactc ttgtactaac
     4861 gaggatcgat ctccctatga agaagcaata cctcaatccc aaagaaatga gcacgccgcg
     4921 cttctacagc cacgcggtca cggccgaagg cacgggaaag cgggtctacg tatcggggca
     4981 ggtgagctgg gacgcagccg gcaacgtggt cggcaagggc gatatgcgcg cgcaaagcga
     5041 gcaggtgttc aagaacgtcg ccgcggcgct gaaggcggcg ggcgctggct ggggcgacgt
     5101 catcaagatg aacggctaca tggtcggcat gaacgccgag cgggtgggcg cttaccgcga
     5161 ggtccggcag cgctttctca aggagggggc gctgccggcg agcacgctgg tcggcgtcga
     5221 agcgctggtc catcccgacc tgctgctgga agtcgaagtc atcgcggcga tttgagccag
     5281 ggcaattacg gctgggtggt ggtcgcggcg gccttcaccc tgatgctcgt cggcttcagc
     5341 gccgcgtact cgttcgcggc cttcttcacc gcttttgaag cggagttcgg cgcttcgcgc
     5401 ggcgacatcg cgctcgtgtt ctcggtcgcg gcgtttctct ggttcctgtt gggcgcgccc
     5461 gcgggggtgc ttgccgaccg ctacggcgtg cgcaaggtcg cgctcgtcgg ggtcgcctgt
     5521 cttgcgctgg cgctatggct ggcgagccgt gcgcagtcgc tcgcgatgct ctacgccact
     5581 tacagcatcg gcatcgggct cggcgtcggc ctcacctacg tgccgtcgat cggcgcggta
     5641 cagccgtggt tcgcggcgaa ccgcgcgttc gcctccgggc tcgcggtcgc cggtatcggc
     5701 gcgggcaaca ttgccgggcc gctgctcgcc gcctggtgga tcgggctttt cggctggcgc
     5761 ggcgcgtacg tggcattggc agtcctggtg ctggtcctcg ggagcgtggc ggcgctcgcg
     5821 attcgcgaaa ccgagcaccg cgcgctcggc acacgcgagg gcgtgaccct tgcggaggcg
     5881 gcgaagacga ccccgttctg gctgctcttc gcctcgctcg tgctgagctg catcgggctg
     5941 ttcgtgccga tggtgcatct cggcccctac gcggtcgacg ccggctacac gcaggcgcag
     6001 ggcgtgacgc tcgtgagcct gatcggcctg ggcagcctgc tcgggcgctt cacgatcggc
     6061 gtcccggccg accggctggg gcgcatgccg tcgctgtcgg cgatgtatgc cgggctcgga
     6121 gcgatgttca tgctgtggtg ggcggccgaa gcctactggg tgctcgcgct cttcgccctc
     6181 gtattcggga tctgctacgg cgcgtacgtg gcgcttctgc cgaccatcgt gatggacctc
     6241 tacgggccgc ggtcggtgtc gggcatcctc ggctgcctct acaccggctg cggcgtcggc
     6301 acgctggtcg ggccctggct cgcgggagtc gcgttcgacg ctcgcggcag ctaccagctt
     6361 ccgatgctgg ccggggctgc gtgctcgctc gccgccgcgg cttgcatcct gcctctgcat
     6421 gggaaagcaa aagccctcta tcgcagttag cgagtcgcgc ggcgtgcgca cgctgcacgt
     6481 cggcggcgag gcgatccaga gctcgatgcg gctcgaggat ccgtacgcgc tcgccctcga
     6541 ctacacgcgc tgcatgatgg cgttcctgct gttccatccc gagccgcgcg aggcgctgat
     6601 gatcgggctg ggcggcggct cgctcgccaa gtttttcttc aggcacctga gaagaacccg
     6661 ggtgcgaatc gtcgagctcg acagccgcat cgtcacggct gcgcgcgagc atttcgcgct
     6721 gccgcccgac ggcgagcgcc tgcgcgtcga ggtgggcgac ggcgccgagg cgctcacgcc
     6781 ggagtgctgc gacgtcctcg tgatcgacgc gttcgaggac gagctgcacg tgccgcggct
     6841 cgcgagcggc gagttctacg acggcgcctt cctcgcgctc aacgagcggg gcgcgctcgt
     6901 ggtcaacttc atgaacgacg atccgaagct cgatgcgacg ctcgagcgcc tcgagcgcgc
     6961 gttcggcggc gcggtgatct gcatgcccgc gctctacgat ccgaacattc ttgcgtttgc
     7021 gttcaagggc gtgtcgcccg ccatttcgtg gagcgcgctg cgcagcagag ccgagcagct
     7081 cgagtcgcgg ctcgggctgc cgttcacgcg ctatgtctcg cgccttcgtg ccatgaaccg
     7141 gtggaatgcg gcggagctta taatcgccgc atgaaattca cgagtgaaag cttcgccgac
     7201 ggcggcgcga tgcccgagca ttgcgcgttc tgcgtgcccg atgccgcgag ccgcgccaag
     7261 ctcggccaga accgcaaccc gcagctcgcg tggtccgatc tgccgaaggg tacgcagtcg
     7321 gtggtgatca tctgccacga tccggacgtg ccgtcgaagg ccgacgacgt caaccaggag
     7381 ggccgcacca tcccggcatc gctgccgcgg gtcgccttta cgcactgggt gctgatcgac
     7441 atgccggcga gcggctcgcc catccgcgac ggcgagttct cgagcggcgt cacgcccaaa
     7501 ggcaagagcg gcccgctcgg ggcgcgcaac acgcgccagg gactcaacga ctacaccgtc
     7561 tggttcagga acgatcccaa catggcgggc gactacttcg gctacgacgg cccgtgcccg
     7621 ccctggaacg actcgatccc gcaccactac gtgtttacgt tgttcgccct cgacgtcgag
     7681 aagtgcccgg tgcagcgcaa gttcacctcc gcccacgtgc tcaaggcgat gaaaggccat
     7741 gtgctgggca ccgcgaaaat caccgggcgc tactcgctga atccggcagt caccgtgtga
     7801 ttgaagcggc ggcgctcgcc gccatcgcct ttgtcggggc gctggtcttc ggcgtgaccg
     7861 gcttcggcgc cgcgctcgtc accattcccc ttgcgacgca cttcgtgccg ctcaagttcg
     7921 cgctggcgct gttcgtcctc gccgacctcg ccgcggcgat cagcgtaggc ctggagaacc
     7981 ccaggaaggc ggtgcgctcc gagtggacgc gcctcgtgcc gatgatcttc gccggcacgg
     8041 cgctcggcgt gacgctgctc gtcaacctgc cgcgcgccgc cggcatggtg ctgctcggcc
     8101 tgttcatcct gtggttcgcg atcctcaacc tcgtgcgccg cgcctcgatg aagatcgttt
     8161 cgccgcgctg ggcgtgggtc gcggggttcg ccggcggcat taccagcacc gtcttcggcg
     8221 ccggcggccc gccgtacgcg atctacctct cgcagcgcgg actgacgaag gagcagttcc
     8281 gcgccacgct ggggttcgcc accctgacca gcatttcgct gcgcttgatc gcctttgcga
     8341 tcaccggtct cctgctcgac cgggaagtct ggctgaaggc gctcgcggtg gtgcccgcgt
     8401 gcctgttcgg catctgggtc gcccgcaaga tcttccggcg catctcgcgc gatgcgctga
     8461 tgcgcgtggt ggccctgatg cttctcgctt cggggacttc gcttattttt cgagcagttc
     8521 tttgagcgcc tcgaggtcgc ggcgcaccca ttgcgtgtcg tcgacgaact tctcctcggt
     8581 cacaccgggc tggcggaata cggtgatcag cagctcggcg ccgtcgccgt tctggaatac
     8641 gcgcatcggc atgtagacct cgggtccgga gccgacggta acgtagtggt cgagcacgcc
     8701 ccacttgttg cgctcgacga agcgcacctt ggccggggcc ccgccggggc ccgaggcaag
     8761 ccccgatgcc cacttgggaa ggttcctggg ctctacgatg aaattgtaga cgtcgagcac
     8821 tgggcactcg atggtcatgc gtacggtgaa gccaggtttt gttttcaggc ggcgcgctgc
     8881 tcccgggagc gctcggccgt ggcgaggtcg cagttcgccg ggccgctgat caccttacca
     8941 tagcgatcga agcgcgaccc gtggcacggg cagtcccagg tgatctcggc cgggttccag
     9001 tgcaccacgc agccgaggtg cgggcacttg gcgaggcact cgtggacctc gccctgctcg
     9061 tcgcggtaaa ccgcgatctt ctccaggccg cggcgcacga tggctccgct cccggggcgg
     9121 acgtcgtccg ccgaagcgac gtcgccgccg gtcagccagt ccgtgtactg cagcgccacg
     9181 ttggagttct cccgcgcgaa gtcgcccaca gcccgcaggg tgacgcgcga ggggtcgtag
     9241 agcgcctccc aggggttggt gaggccgagg atcaggtcgc tgagcagcat gccggcgatg
     9301 gtcccgtggg tgattcccat gcccgagtcg ccggtggcca cgtagacgtt ttcgtggtcc
     9361 atcgggttgc ggccgataaa agcgagaaag tcctgcgtct ccatgacctg gccgccccag
     9421 gcgagctcga cctcgcccat ctccggaaac cgtgcccggg cccaggcttc gagccgcgcg
     9481 tagcgctcgg gcgtgtcgct cgcctggccg gttttgtggt cctcgccgcc gacgatgagc
     9541 agatcatcgt aaagacgaag atagtggtag gggtcgccgg tatcccagct cagcaccgtc
     9601 ggcaccgagc cctgcggcac gcgggctgcg accacatagg tcatgtaagg cgcctgcttg
     9661 gtatggatcg cgacgcggtc gttgaccggg acgttggttg cgacgaccac ggcatcggcc
     9721 gtgacgacgt gcttcccggc gtgcaccagc gccggaaccc cgccctcgat ctggtcggcg
     9781 tgggtacccg tgaagatgcg gctgcctgcg cgatggatgg cttcggcgag cgccgagagg
     9841 tacttgagcg gatggaactg tccctgagcg gggaagcgca ggcagggtcc ggggtcgaac
     9901 gaccggaaag gcgcgcgctc gaggagctcc accgcacgca acccggcgcg atgcgcggcc
     9961 tcgagctcgt gctcgagcgt cccgcgcgtg tcgccctcgg cgagaaacag gtagccgtcc
    10021 acgcgcgcga aatcgcaatc gatgctctcc tgccgcacga tggtctcgat gcggtctatc
    10081 gccgcggtgt ggctgtcggc ggcgaggcgc gcgccttcgc ggccgtggag cttctccagc
    10141 tcgaagtagc ggtcgtcgag ctggttggtg atgtgggcgg tcgtcatctg cgtcatcccg
    10201 cccgcgaccg gtccatcgtc gagcaccgcc acgcgcttgc cggcgcgcgc gagcacatag
    10261 gcggtggtca ggcccgaaat gccggcgccg acgatgcaga cgtcgacgtg gatgttttgc
    10321 tccaggggtg taaagctcgg aagcgcggcc gtggccgccc agatcgattg gtggcgtcgc
    10381 atagtgacgt cactccagca agaaacgatc ctacgttgct ctctcccgca tggtcacgaa
    10441 ttcctcggcc gccgtcggat ggatgccgat ggttgcgtcg aactgcgcct tggtcgctcc
    10501 aagcttcacc gcaatcgcca cgccctggat gatttcccct gcgtcggcgc cgatcatgtg
    10561 cgcgccgacc acgcgctggc tcgcccggtc gaccacgagc ttcatgaagg tggtgtcggg
    10621 cttgtcgctc atgacgacct tgagcgcgcg gaacgaggct ttgtagatgt cgaccgcgcc
    10681 gaagcgctcg cgcgcccgct cttccgagag cccgaccgtc gcaaggttcg gattggcgaa
    10741 caccgccgtc ggcacgttct cgtggtcgac cggcgtcggc tcgccgcgaa acagcgtcct
    10801 ggcgagccac atcgcttcgg cggtggcgac cggcgtgagg ttgatgcggt tcgtgacgtc
    10861 gcctacggca tgaatggaat ccacattcgt tcttgaaaac cggtcgacga tgatcgcccc
    10921 gtcggcggcg agcttcacgc cggcggattc caggccgagc ttcgccgtgt tgggacgccg
    10981 gccggtggcg aacatcacca ggtcggtccg ttcgatcctg ccgtcggtga attcgatttc
    11041 aagatcattt ctcttgcgga tggcggccgg ctcgcagccg aagcacacgt tcacgccctt
    11101 cgccgacatc tcttgcgcga tgcgcgtgcc gagctccgcg tcgaagcctc gcagcaggcg
    11161 cttgccgcgg taggtgagca tcgtctcgac gcccaggccc tggaagatcg aggcgaattc
    11221 gagcgcgatg tagccgccgc ccaccacgac cgcgcgcttc ggcagccgct cgagggcgaa
    11281 cgcctcgttg gaggtgatgg cgtgctcgat gccgggaatg acggggagct gcggccagga
    11341 gccggtcgcc acgagaatgt gttttgccgt tcgcttcttg ccgtcgatct cgacttcgtt
    11401 cggtccaagg accgtggcgc gcaccctgtg gatctcgacg cctgcattca ccagcacgcg
    11461 ctcgtacacg ccgttcaggc gcgcgatctc gcgatccttg ttcttgagaa gcgtctgcca
    11521 gtcgaagcgc agtgggtggg aagatgagcc gaccgtccag ccgtacgcct cggcgagctt
    11581 gaaatcctcg cgccaatgcg cggcgtagga aaagagcttc ttcggaatgc agccgacgtt
    11641 gacgcaggtg ccgccgaagc gtcccggctc ggcgatggcg accttcgctc cgtaggcggc
    11701 gcagacgcgg ctcgcgcgca ctccgccgga gccgccgcct atggtgaaca ggtcgtaatc
    11761 gaagcccagg attacgcggc ggttctactt caggttgggc aggttgaggc cgaccagctc
    11821 gagctgcgag gcgatgcggt tgagctgcgg cttgtcggcg agccacttca gcgcgttctt
    11881 caccttctgc tcgatctgca cgcgcggcat gccctcgatg ccgt
//
LOCUS       DATBSZ010000265         9071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_871968, whole genome shotgun sequence.
ACCESSION   DATBSZ010000265 DATBSZ010000000
VERSION     DATBSZ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871968"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1935
                     /locus_tag="VIG70_17030"
     CDS             220..1935
                     /locus_tag="VIG70_17030"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY8252299.1"
                     /translation="MQTTGFLKAFWALTKPYWVSRERRVGLTLLATVVGLALFGVWLE
                     VQFNTWNREFYNTFESRDQAEFFRQLGMFALLALIWIITGVYRLYFLQMLQIEWRTWL
                     TDRFLGAWMKDQAHYRMQLLARAKDTPVAGMDNPDQRIAEDLKLFVDYTLSLSLGLLS
                     AVVTLVSFILILWTLSGSLELFGVDIPGYMVWAALVYAIAGSVLTHVIGRPLIRLDFN
                     QQRFEADFRFSLVRLRENSEGVALYRGEPEELGGLRGRFAAVIGNWWQLMLKQKQLGW
                     FTSFYAQLAIIFPYIVVSPRFFAGQIPLGQIFQTASAFGQVQGALSWFIGAYTQFASW
                     KATSDRLIGFSAVLEQVRADALKVPGERAAAAGDSLGVADLALELPQGSTLLAPTTLE
                     FKPKRDVLISGPSGSGKSTFFRALAGIWPYWKGRISLPRGARMLFLPQKPYLPIGSLK
                     HAVGYPDPASKFTDAEASDALRAVGLGQLAGELERSENWAQVLSGGEQQRLAFARALL
                     YRPDWLFLDEATASLPEEDQDKLYALLKERLPGTTLVSIGHRETLARYHDERLVWRGS
                     ALVPA"
     gene            complement(1945..2424)
                     /locus_tag="VIG70_17035"
     CDS             complement(1945..2424)
                     /locus_tag="VIG70_17035"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY8252300.1"
                     /translation="MKRTLEVTWDDPMRLAQAGRSMSGIEFLRAIRDGKLPHPPICSL
                     LNYRLVDVEPGHVVFEITAGEQHYNPIGVVHGGIAMTLLDSAMACAVQTQMPAGGGYT
                     TLETKTNLVRAVTADTGKLRAIGKVLHAGKRIATAEGRLEDAAGKLYAHATTTCIAL"
     gene            complement(2424..3305)
                     /locus_tag="VIG70_17040"
     CDS             complement(2424..3305)
                     /locus_tag="VIG70_17040"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8252301.1"
                     /translation="MSAAFSLSRISTNVRFSASALLFPELAGAWAERMFLTPPRARDA
                     AAAALDLIDARSTLLEHKGRHIAMWRWGSRDAPAVLLAHGWGGNAAQMRPFVFPLLQA
                     GYRVIAYDQPAHGVSEGKLTGLPDFADVLSEIALHHGGVQAVIGHSLGAAAAAVSLSS
                     GKTFFGKVVLVSPPSDLVGYSRRFARWYWMPEPVRRAMQKAIEERYGVRWEDLEVPRV
                     APRLAAQALVIHDRGDRLMPWIHGATVARHWPRARLLTTEGLGHRRILADERVTRAAA
                     DFVAGRASLVDSPAPLY"
     gene            complement(3324..3956)
                     /locus_tag="VIG70_17045"
     CDS             complement(3324..3956)
                     /locus_tag="VIG70_17045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8252302.1"
                     /translation="MTAPTKGEQTRAAILDEALRIASKLGLEGLTIGSLADATGMSKS
                     GLFAHFGSREDLQLAVLEHAAQRYGEQVFVPVLKIERGLPRLRALFERWLDWTIESGL
                     PGGCIMISAAAEYDDRPGPIRDAVIANQHRGNAISQKAVRLAIEEGHLSPGTDPEQIS
                     FEMLGIVLASHNHRRLLGDREARKRALTAFDELISRHAAPARVRARAATR"
     gene            complement(3953..6796)
                     /gene="uvrA"
                     /locus_tag="VIG70_17050"
     CDS             complement(3953..6796)
                     /gene="uvrA"
                     /locus_tag="VIG70_17050"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY8252303.1"
                     /translation="MDSIRVRGARTHNLKNLSLELPRNRLVVITGLSGSGKSSFAFDT
                     LYAEGQRRYVESLSAYARQFLELMAKPEVDLIEGLSPAIAIEQKAASHNPRSTVGTVT
                     EIHDYLRLLYARAGTPYCPEHALALEAQSVAEMVDQVAALPPESRVMILAPVVAGRKG
                     EQADLLEELRAQGFTRVRIDGTVRDLDSAPRLAKNAKHSVDVVVDRLRVRPDARQRLA
                     ESLETALRHADGKVVVVEADNGKEHLFSARYSCPACDYSLPELEPRLFSFNNPAGACP
                     RCDGLGAVEFFDPARIVAHPNLSLASGAIRGWDRRNHFYYAMLQSLARHYRFDIESPW
                     ESLDDRIQSLILYGSGKDKIAFHYPSERGRSAVKEHAFEGVIPNHERRYRETDSMAVR
                     EELAKLRNVRSCPECGGTRLRREARHVRVAEKTIFEVSRWPLRQTLEFFGHLRLQGAK
                     GQVAERVVREIASRLQFLVNVGLEYLSLERSAESLSGGEGQRIRLASQIGSGLTGVMY
                     VLDEPSIGLHQRDNARLIGTLQQLRDLGNSVIVVEHDEEAIRAADYVVDMGPGAGEAG
                     GRIVAQGSPSQVIANPESLTGRYLARELTIAVPERRRRSKQKIVLEGAHGNNLKEVDL
                     ELPLGLLVCVTGVSGSGKSTLINDTLYAAVARTLYGAAAEPAPHRALTGVEQVDKVIS
                     VDQSPIGRTPRSNPATYTGLFTPIRDLFAGVPEARQRGYDTGRFSFNVKGGRCEACQG
                     DGVTRVEMHFLPDLYVACDVCHGKRYNRETLEIRYKGRNVHEVLEMTVAEAHSFFSAV
                     PPIARKLQTLTDVGLGYVRLGQSATTLSGGEAQRVKLSLELSKRDTGRTLYILDEPTT
                     GLHFHDIKLLLEVLHRLRDAGNTVVVIEHNMEVIKTADWVIDLGPEGGERGGRIVAAG
                     TPEQVSEIPESHTGRYLKQVLTFTNVRSILAPV"
     gene            6874..8226
                     /locus_tag="VIG70_17055"
     CDS             6874..8226
                     /locus_tag="VIG70_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8252304.1"
                     /translation="MTSVEVRAGASLAGVFGLRMLGLFFILPVLAVHAANIRGGDDLT
                     LVGVALGAYGLSQGILQIPFGMASDRWGRKPVLYAGLAVFAAGSFLGMIAHDIWTMIG
                     ARTLQGAGAISSVAMALAADLTRDQHRTKVMAMIGSMIGLMFALSLVGAPLLYRAIGM
                     DGLFALTGALCLAAMAVVKFQVPDPPAHGRAAPAAGSVRAALLDAELVRLNAGIFILH
                     VVLYAMFVVVPPLLVQAGLALPEHWKLYLPVVLGSFVLMVPAVLYADRRNASKPVLVG
                     AVALLVGAEAAFAVLGGGIAVLAAVMLGFFVAFNVLEALLPSLVSRRAPAAGRGAAIG
                     VYNTTQTLGVFFGGLLGGWVASRYGTTGVFAACAVLSAVWLVLAAGMRHPPAVNELSS
                     LTFPIASGVNLDGLREALARVKGVREAEVVAAERIARLKVVPGQWDEHRVRKLITGEV
                     "
     gene            8229..8696
                     /gene="ssb"
                     /locus_tag="VIG70_17060"
     CDS             8229..8696
                     /gene="ssb"
                     /locus_tag="VIG70_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY8252305.1"
                     /translation="MASVNKVILVGNLGRDPETRYMPDGAAITNVSVATSFQWNDKTS
                     GEKKEETEWHRVVFRAKLAEIAGEYLKKGSQVYIEGRLRTRKWQDKEGQDRYTTEVVA
                     ERMQMLGSRAGSGEPRGEPRGEPAMESRTAEPKPAAAKKPAGKFDDMEDDIPF"
     gene            8718..9023
                     /locus_tag="VIG70_17065"
     CDS             8718..9023
                     /locus_tag="VIG70_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY8252306.1"
                     /translation="MPIYEYRCSACGQELEVLQKVTEPPLTDCPSCGKPGMQKMLTAA
                     GFQLKGGGWYATDFKGGSKKKPEEKGKPETKTDTKTDAKPDQKPDTKTETKSTACTD"
BASE COUNT         1425 a         2974 c         3110 g         1562 t
ORIGIN      
        1 caccagcgcc gcgctcggaa agcgcgcctg gaacttgaac gcgatcggga tcatcgattg
       61 cgcggtggcg ccgcaaccgt gcaggaagac gaacaggtgg tttggccggc gctctggcgt
      121 gggggcgaag cggaaaaccc ggcccgaaga cattgccccc gattgttaac ccgtgacgac
      181 agttctgtaa actgatttgc attccctctt aggtgccgca tgcaaaccac aggatttcta
      241 aaggctttct gggcgctcac caagccgtac tgggtgtcca gggagcgccg cgtcgggctg
      301 acgctgctcg ccaccgtggt cggcctggcg ctcttcggcg tgtggctcga ggtgcagttc
      361 aacacctgga atcgcgagtt ctacaacacg ttcgagagcc gcgaccaggc ggagtttttc
      421 cggcagctcg ggatgttcgc gctgctcgcg ctgatctgga tcatcaccgg cgtctaccgg
      481 ctgtacttcc tgcagatgct gcagatcgag tggcgcacct ggctcaccga tcgcttcctc
      541 ggcgcgtgga tgaaggacca ggcgcattac cgcatgcagc tcctcgcgcg cgccaaagac
      601 acgccggtcg ccggcatgga caaccccgac cagcgcatcg ccgaggacct gaagctgttc
      661 gtcgactaca cgctgtcgct gtcgctcggc ctgctgtccg cggtggtgac cctggtgtcc
      721 ttcatcttga tcctgtggac gctgtcgggc tccctcgagc tcttcggcgt ggacatcccc
      781 ggctacatgg tctgggcggc gctggtctat gcgatcgcgg gcagcgtgct cacgcacgtc
      841 atcggccggc cgctgatccg gctcgatttc aaccagcagc gcttcgaggc cgacttccgg
      901 ttctcgctcg tgcgcctgcg cgagaacagc gaaggcgtgg cgctctatcg cggcgagccg
      961 gaggagctcg gcggcctgcg cggccgtttc gccgccgtga tcggcaactg gtggcagctg
     1021 atgctcaagc agaagcagct cggctggttc acttcgttct atgcccagct cgcgatcatc
     1081 tttccctaca tcgtggtgtc gccgcgcttc ttcgccggcc agatcccgct cgggcagatc
     1141 ttccagaccg cctcggcatt cggccaggtg cagggggcgc tctcgtggtt catcggcgcc
     1201 tacacgcagt tcgcttcctg gaaggcgacc agcgaccgcc tgatcggctt ttccgcggtc
     1261 ctcgagcagg tgcgcgcgga cgcgctcaag gtgccgggcg agcgcgccgc ggcagcgggc
     1321 gacagcctgg gcgtggcgga cctcgcgctc gagctgccgc aaggatcgac gctgctcgcg
     1381 ccgactacgc tcgaattcaa gccgaagcgc gatgtgctga tcagcgggcc ttcgggttcg
     1441 ggcaagtcga cttttttccg cgccctcgcc ggcatctggc cctattggaa aggccgcatc
     1501 tctctcccgc ggggcgcgcg catgctgttc ctgccgcaga agccttacct gccgatcggc
     1561 tcgctcaagc acgcggtggg ctatcccgac ccggcgagca agttcacgga tgccgaagcg
     1621 agcgatgcgc tgcgcgcggt cgggctcggc cagctcgcgg gcgagctcga gcgcagcgaa
     1681 aactgggcgc aggtgctctc gggcggcgag cagcagcgcc tcgcgttcgc gcgcgcgctg
     1741 ctttaccgac ccgactggct gttcctcgac gaagcgacgg cgtcgcttcc agaggaggac
     1801 caggacaagc tctatgcgct gctgaaggag cgactgcccg ggacgacgct ggtctcgatc
     1861 gggcatcgcg agacgctggc gcgataccac gatgagcgcc tcgtctggcg cgggtcggcg
     1921 ctggttcccg cctagccgaa gccgctacaa ggcgatgcag gtcgtggtgg cgtgcgcgta
     1981 gagcttgccc gcggcatcct ccagccggcc ctctgcggtc gcgatgcgct tgccggcgtg
     2041 cagcaccttg ccgatcgcgc gcagcttgcc ggtgtcagcg gtcaccgccc gtaccaggtt
     2101 ggtcttcgtc tcgagcgtgg tgtagccgcc gccggccggc atctgtgttt gcaccgcgca
     2161 agccatcgcg gaatcgagca gcgtcatcgc gatgccgccg tgcaccacgc cgatcgggtt
     2221 gtagtgctgc tcgccggccg tgatctcgaa gacgacgtgg ccgggctcca cgtcgaccag
     2281 ccggtagttg aggaggctgc aaatcggcgg atgcggcagc ttgccgtcgc ggatcgcgcg
     2341 caggaactcg atcccggaca tcgagcgccc ggcttgcgcg agccgcatcg ggtcgtccca
     2401 ggtgacttcg agcgttcgct tcatcagtag agcggcgcgg ggctgtcgac gagcgaggcg
     2461 cgcccggcga cgaaatccgc cgcggcgcgg gtgacgcgct cgtcggcgag gatgcgccgg
     2521 tgtccgaggc cttcggtcgt gagcagacgt gcgcgcggcc agtggcgcgc gaccgttgcg
     2581 ccgtggatcc atggcatcag gcggtcgccg cggtcgtgga tcaccagcgc ctgggcggcg
     2641 agacgcggtg cgacgcgggg aacctccagg tcttcccaac gcacaccgta gcgctcctcg
     2701 atcgctttct gcatcgccct gcgcaccggc tcgggcatcc agtaccagcg ggcgaagcgg
     2761 cgcgagtagc cgacgaggtc ggaaggcggg ctgaccagca cgactttccc aaaaaaagtt
     2821 tttccggaag aaagcgaaac cgccgccgca gcggcgccaa gcgaatgacc aatcaccgcc
     2881 tgcacgccgc cgtggtgtag cgcgatctcg gacagcacgt cggcgaagtc gggcaatccg
     2941 gtgagcttgc cttcggacac gccgtgcgcc ggctggtcgt atgcgatcac gcggtagccg
     3001 gcctgcagca gcggaaatac gaacgggcgc atttgggcgg cgttgccgcc ccagccgtgc
     3061 gcgaggagta ccgcgggcgc atcgcgcgag ccccagcgcc acatcgcgat atgacggcct
     3121 ttgtgctcga gcagggtgga gcgcgcatcg atcaggtcaa gcgctgcggc cgcggcgtcg
     3181 cgcgcgcgcg gcggcgtcag gaacatgcgc tcggcccagg cgcccgccaa ttccgggaac
     3241 agcagcgccg aagcagagaa tcgaacgttc gtgcttattc tggacaggga gaaggcggcg
     3301 gacatggcgg cttcctttcg ggtttagcgg gtcgctgcgc gggcgcgaac gcgtgccggc
     3361 gcggcgtggc gggagatgag ctcgtcgaat gccgtaagcg ctcgcttacg cgcttcccgg
     3421 tctccgagca gacgtcgatg gttatggctc gcgagaacga tgccgagcat ttcgaagctg
     3481 atctgctcgg ggtcggttcc tgggctgagg tgcccttcct cgatggcaag gcggaccgcc
     3541 ttctggctga tcgcgttgcc acggtgctgg ttggcaatga ccgcgtcccg gatcggccct
     3601 ggccggtcgt catactcggc ggccgccgag atcatgatgc agccgccggg gaggccggac
     3661 tcgatcgtcc agtcgagcca gcgctcgaaa agggcgcgca gccgcggcag gccgcgctcg
     3721 atcttcagca ccggcacgaa cacctgctca ccgtaacgct gggccgcgtg ctcgagcacc
     3781 gcgagctgca ggtcttcgcg cgagccgaaa tgcgcgaaca gccccgactt cgacatgccg
     3841 gtggcgtcgg ccaggctgcc gatcgtcagc ccctcgaggc cgagcttgct cgcgatcctg
     3901 agtgcttcgt cgaggatcgc ggcgcgggtc tgctcgcctt tggtcggggc cgtcatacag
     3961 gcgccaaaat agaacgaacg ttcgtaaatg tcaagacctg cttcagatag cggccggtat
     4021 ggctttccgg gatttcagaa acctgttcgg gggtcccggc agccacgatg cggccgccgc
     4081 gctcgccgcc ttcgggcccg agatcgatca cccagtcggc ggtcttgatc acttccatgt
     4141 tgtgctcgat cacgaccacg gtgttgccgg catcgcgcag gcggtgcagc acctcgagca
     4201 ggagcttgat gtcgtggaaa tgcagccccg tggtcggctc gtcgaggatg tagagcgtgc
     4261 ggccggtatc gcgcttggaa agctcgagcg agagctttac gcgctgcgcc tcgccgcccg
     4321 agagggtggt cgccgactgt ccgaggcgca cgtagcccag gccgacgtcg gtcagcgtct
     4381 ggagcttgcg cgcgatcggc ggcaccgcgg agaagaagga atgcgcctcg gcgaccgtca
     4441 tctcgagcac ttcgtggacg tttcgcccct tgtagcggat ttccagcgtc tcgcggttgt
     4501 agcgcttgcc gtggcagaca tcgcaggcga cgtagagatc gggcaggaag tgcatctcga
     4561 cccgcgtgac gccgtcgccc tggcaggctt cgcagcgccc gcccttcacg ttgaacgaga
     4621 agcggcccgt gtcgtagccg cgttgccgcg cctcgggcac gccagcgaaa aggtcgcgga
     4681 tcggcgtgaa caggccggtg taggtcgccg ggttggagcg cggggtgcgc ccgatcgggc
     4741 tctggtcgac gctgatcacc ttgtcgacct gctcgacgcc ggtgagcgcg cgatgcggcg
     4801 ccggctccgc cgccgcgcca tacagcgtgc gcgcgaccgc ggcgtagagc gtgtcgttga
     4861 tgagcgtgga cttgcccgac cccgacacgc cggtgacgca gacgagcagc ccgagcggca
     4921 gctcgagatc cacctctttc agattgttgc cgtgcgcgcc ttcgagaacg atcttctgct
     4981 tcgagcggcg gcgccgctcg ggcaccgcga tcgtgagctc gcgcgcgaga tagcggccgg
     5041 tcagcgattc cggattggca atgacctggg aaggcgaccc ctgcgcgacg atgcgtccgc
     5101 cggcttcgcc cgcgcccggg cccatgtcga ccacgtagtc ggcggcgcgg atcgcctcct
     5161 cgtcgtgctc caccacgatc accgagttgc cgaggtcgcg cagctgctgg agcgtgccga
     5221 tcagtcttgc gttgtcgcgc tggtgcaggc cgatcgaggg ctcgtcgagc acgtacatca
     5281 cgcccgtcag cccggagccg atctggctcg ccagccggat gcgctgccct tcgccccccg
     5341 aaagcgattc ggccgagcgc tcgagcgaga gatactcgag gcccacgttc accaggaact
     5401 gcagccggct cgcgatctcg cgcaccacgc gctccgcaac ctggcccttt gcgccctgga
     5461 gcctgaggtg tccaaagaac tccagcgtct gccgcagcgg ccagcgcgac acttcgaaga
     5521 tggttttttc agcgacccgg acgtgacgcg cctcgcggcg caggcgcgtg ccgccgcatt
     5581 ccggacagga gcgcacgttg cgcagcttgg cgagctcttc gcgcactgcc atcgaatcgg
     5641 tctcgcggta gcggcgctcg tggttcggga tcacgccctc gaaggcgtgc tccttcaccg
     5701 cgctgcggcc gcgctcggag gggtagtgga aggcgatctt gtccttgccc gagccgtaca
     5761 ggatgaggct ctggatccgg tcgtcgagcg actcccacgg gctctcgata tcgaagcggt
     5821 agtgccgggc gagcgactgc agcatcgcat agtaaaaatg atttcggcga tcccagccgc
     5881 ggatcgcgcc gctcgcgagc gacaggttgg gatgcgcgac gatgcgcgcg gggtcgaaga
     5941 actccaccgc gcccaggccg tcgcagcgcg gacacgcgcc ggccgggttg ttgaaggaga
     6001 acaggcgcgg ctcgagctcg ggcagcgagt agtcgcacgc cggacacgaa taccgggctg
     6061 aaaagaggtg ctccttgccg ttgtccgctt cgactacgac gaccttaccg tcggcgtgcc
     6121 gtagcgcggt ctcgagggat tcggccagcc gctgcctcgc atcgggccgc acccgcagcc
     6181 ggtcgacgac gacgtcgacc gaatgcttgg cgttctttgc gagtcgtggt gccgaatcga
     6241 ggtcgcgaac cgtgccgtcg atgcgtaccc gggtgaatcc ctgcgcgcgc agctcttcca
     6301 gcaggtccgc ctgctcgcct ttgcggccgg cgacgaccgg cgcgaggatc atcacccggc
     6361 tctctggcgg cagcgccgcg acctgatcaa ccatctctgc cacgctctgc gcttcgagcg
     6421 cgagcgcatg ctccgggcag tagggcgtgc cggcgcgcgc gtagagcagg cgcagatagt
     6481 cgtggatctc ggtcaccgtg ccgacggtgg agcgcgggtt gtggctcgcc gccttctgct
     6541 cgatcgcgat cgcgggcgac agcccctcga tcagatccac ttccggcttc gccatcagct
     6601 cgaggaactg gcgcgcgtag gccgacagcg actcgacgta gcgccgctgg ccttcggcgt
     6661 acagcgtgtc gaacgcaaac gagcttttgc ccgagcccga gagtcccgtg atcacgacga
     6721 gccggttgcg cggcagctcg aggctgaggt tcttcaggtt gtgcgttcgc gcgccgcgaa
     6781 cgcggatgct gtccatctgc aatttcgaaa gtgaaaccgg ctactatagc gcgtctcgca
     6841 ttcccgcctc cacagctatc aatgaccgaa agaatgacca gcgtcgaggt gcgcgcgggc
     6901 gcgagcctcg ccggcgtttt cggcctgcgc atgctgggcc tgttcttcat ccttccggtg
     6961 ctcgcggtgc acgcggcgaa catccggggc ggcgacgacc tcacgctggt cggcgttgcg
     7021 ctcggcgcct acggcctttc gcagggaatc ctgcagatcc cgttcggcat ggcctcggac
     7081 cgctgggggc gcaagcccgt gctctatgcg ggcctcgcag tgttcgccgc gggcagcttt
     7141 ctcggcatga tcgcgcacga catctggacg atgattggcg cgcgcaccct gcagggcgcc
     7201 ggggcgatct cgtcggtcgc gatggcgctc gccgcggacc tcacgcgcga ccagcaccgc
     7261 acgaaagtga tggcgatgat cggctcgatg atcgggctca tgttcgcgct gtcgctggtg
     7321 ggggcgccgc ttctctaccg cgcgatcggc atggacggcc tgttcgccct caccggcgcg
     7381 ctttgcctcg ctgcgatggc ggtggtgaag ttccaggtgc ccgatccgcc ggcgcacggc
     7441 cgcgccgcgc ccgccgcggg ctcggtgcga gctgcgctgc tcgacgccga gctcgtgcgc
     7501 ctgaacgccg gcatcttcat cctgcatgtc gtgctttacg cgatgttcgt cgtcgtgccg
     7561 ccgctcctgg tccaggcggg gctcgcgctg cccgagcact ggaagctgta tctgccggtc
     7621 gtgctggggt cgttcgttct gatggtgccg gcggtcctgt acgcggaccg gcgcaacgct
     7681 tcgaagccgg tcctggtcgg ggcagtcgcg cttttggtcg gcgccgaggc ggcgttcgcc
     7741 gtcctcggcg gcggcatcgc ggtcctcgcc gccgtgatgc tcggattctt cgtggccttc
     7801 aacgtgctgg aggcactgct gccgtcgctc gtttcacgcc gcgcgccggc agcgggccgc
     7861 ggcgctgcca tcggggtcta caacaccacg cagactctgg gagtgttctt cggtggactg
     7921 ctcggcggct gggttgcttc gcgctacggg actaccgggg tattcgccgc ctgcgctgtt
     7981 ctgtccgcag tatggctcgt gctcgcagcc ggcatgcggc acccgcctgc ggtcaacgag
     8041 ctttcgagtc ttacgtttcc cattgcatcg ggagtgaacc tcgatgggct gcgcgaggcg
     8101 ctggcgcgag tgaagggcgt gcgcgaggcg gaggtcgtgg ctgcggagcg catcgcccgg
     8161 cttaaagtcg tgccggggca gtgggatgag catagagttc gtaaactgat tactggggag
     8221 gtctgactat ggcatcggta aacaaggtca ttctggtcgg caatctcggg cgcgatccgg
     8281 aaacgcgcta catgcctgac ggcgcggcca ttaccaacgt ttcggtggcg acgtccttcc
     8341 aatggaacga caagacgagc ggcgaaaaga aagaggaaac cgagtggcat cgcgtggttt
     8401 tccgcgcaaa gcttgccgag atcgccggcg agtacctgaa gaagggctcg caggtgtaca
     8461 tcgaggggcg cctacgcacg cgcaagtggc aggacaagga aggccaggac cgctacacga
     8521 ccgaagtggt cgcggagcgc atgcaaatgc tcggctcgcg cgcgggctca ggggaacccc
     8581 gcggcgagcc ccgcggtgag cccgccatgg aatccaggac cgccgagccg aaaccggctg
     8641 cggcgaagaa gcccgccggc aagttcgatg acatggaaga cgatattcct ttctgaaatc
     8701 agcgatataa tcgcggcatg ccgatctacg aatatcgctg cagcgcgtgc ggccaggagc
     8761 tcgaggtcct gcagaaggtg accgagccgc cgctcaccga ctgcccctcc tgcggaaagc
     8821 cgggcatgca gaagatgctg accgcggccg gcttccagct gaaagggggc ggctggtacg
     8881 ccaccgactt caagggcggg tcgaaaaaga agcctgagga aaaaggcaag ccggaaacga
     8941 aaaccgatac caagaccgac gcgaaacccg atcagaaacc cgacaccaag accgaaacca
     9001 aatccacagc ctgtacggac tgaggctcgg caagtaacct agctggcccg gcgcgcgccg
     9061 ggcctttttg t
//
LOCUS       DATBSZ010000266        43928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1212999, whole genome shotgun sequence.
ACCESSION   DATBSZ010000266 DATBSZ010000000
VERSION     DATBSZ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 43928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43928
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212999"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VIG70_17070"
     CDS             complement(<1..1118)
                     /locus_tag="VIG70_17070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY8252307.1"
                     /translation="MPVVTCIDDLRELSRKRVARAIFDYVDRGSYSEATLRANRADLE
                     LLSLRQRVGIDVDRRSTRTTMAGHDVAMPVALAPVGLTGLNWADGEILAARAAARFGV
                     PYTLSTMSICSIEDVAAGTGKPFWFQLYVMRDRGFAASLIERAKAARCSALVLTLDLQ
                     IQGQRHRDLKNGLAVPPRLTAATVFDVLRKPGWALNVLRGKRKSFGNLEGRIPDAKNL
                     TTLSQWIAGQFDPTLSWKDVEWVKNLWGGKLILKGILDAEDAKIAASSGADAIVVSNH
                     GGRQLDGAVSSIRALPEIAEVVNNKIEVWVDGGIRSGQDVLKALALGAKATMIGRAYA
                     YGLGALGEAGVTRALEIIRAELDVTMGLCGLRDVKDASP"
     gene            1245..1502
                     /locus_tag="VIG70_17075"
     CDS             1245..1502
                     /locus_tag="VIG70_17075"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEY8252308.1"
                     /translation="MEQRACPKGRSVGDGLLFRVSPAGKASWAMRYRFNGQPRYFPIG
                     RYPAVSLEAARIAAKKAWAKIYDGAADGVAENDLLDPTIGA"
     gene            1688..2170
                     /locus_tag="VIG70_17080"
     CDS             1688..2170
                     /locus_tag="VIG70_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252309.1"
                     /translation="MLRNLLLAGLAVLVFVSCGGGDDEEEPPPNRGHLTIGNPSADAV
                     TVAPSETTIELSGTAFNASFFFNCFFGTNHVADIGVSVTWNNAAGGSGTASQSVGCCS
                     ITGTGGKGWLCALHGGSGTVGDHNWSASIPIIPGTNVVTMTASDSSGNVGRDTITITR
                     "
     gene            2374..3165
                     /locus_tag="VIG70_17085"
     CDS             2374..3165
                     /locus_tag="VIG70_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY8252310.1"
                     /translation="MSANDEHKSPIQVIDRMMRLLDVLAQHPEPLGLKQISQYTGLHP
                     STAHRILSSMSADRIVDRVEPGSYRLGMRLLELGNLVKSRISVRELALPMMRELHSQT
                     GETANLSVRHGDEIVYVERTSSGRSAMRVVHVIGARAPLHVTAAGKLFLLEEGFARLR
                     DYAKRTGLAAHTKNTLASLTLLERELERIQRQGWATDNEEAEIGVRCVAAGIRDDGGN
                     LVAALSLSTPADRMKVQWGPLVKEAAERISRTIGHREPVARIGAH"
     gene            complement(3641..4576)
                     /gene="egtD"
                     /locus_tag="VIG70_17090"
     CDS             complement(3641..4576)
                     /gene="egtD"
                     /locus_tag="VIG70_17090"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEY8252311.1"
                     /translation="MARERGRNRPLELASFRNDVVAGLSLPQKSLPPKYFYDAAGSRL
                     FARICRLPEYYLTRAELALTRRHLPAIVRFAGRGCQLVEYGSGEGVKSRMLIRALRPS
                     VYVPIDISEDALLAAARGLAREFPWLRVAPLVGDFSRPLDIRVRRGTPSVVYFPGSTI
                     GNLAPEEAHAFLSMSRGVAVRMLVGVDLKKDPALLHAAYNDAAGVTAAFNLNLLARIN
                     RELGADFDPRRWRHYAFYNPAQGRIEMHLVALAAQRVRLGSHRWHFAAGETIHTENSY
                     KYSLDGFRELAARAGFRSARVWTDRRGLFALHGLS"
     gene            complement(4537..5394)
                     /gene="pbpG"
                     /locus_tag="VIG70_17095"
     CDS             complement(4537..5394)
                     /gene="pbpG"
                     /locus_tag="VIG70_17095"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEY8252312.1"
                     /translation="MQRKLHPLEGIVAMCALVLVLFLSVGFAQAQDVRKIALRSSSAL
                     VQDAETGEVVFGKNADAVVPIASITKLMTAMVILDSGLDLEEQIVLSREDAVAMKGAR
                     SRLRTGLSISRGDLLLLALMSSENRAAAALGHSYPGGLEVFVDAMNAKAAVIEMHESR
                     FVEPTGLSPANVSTASDLAKLVRAAHDYPLIREYSTKDRATIRASKRPLAYGNTNGLV
                     RSKYWDIELSKTGYISEAGRCLVMHVRLAEKDLIVVLLDSWGKHSRIGDANRIKKWLE
                     SGAVTGRSS"
     gene            complement(5584..7395)
                     /locus_tag="VIG70_17100"
     CDS             complement(5584..7395)
                     /locus_tag="VIG70_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY8252313.1"
                     /translation="MAHSAAPTQRLRGNEWKALGTLAPYLWEYKWRVVVALVCLVTAK
                     LANVGVPLVMKEVVDNLDPSLQLVAVPVALLAIYGVLRFSTTLFQELRDVLFVRVTQR
                     AIRRVALGVFRHLHSLSLRFHLDRQTGGMTRDIERGTRGISTLLSYLIFSIFPVILEF
                     ALVAAVLIAKFDWRFAAITFGAVTVYIAFTVTITEWRMEIRRRANELDSRANTRAIDS
                     LLNYETVKYFGNEEFEARRYDEQLQKYEDAATRNEASLGVLNIGQSLIIALAVTALML
                     LAAEGVAARSFTLGDLVLVNGLLIQLYIPLNFLGMVYREIKQALLDMDRMFRLLSAHR
                     EVEDRPGAPDVPHGAATVEFSRVDFSYEEARQILFDVSFTIPAGHRVAVVGHSGSGKS
                     TLARLLYRFYDVSGGSILVNGKDTREVRQASLRAAIGIVPQDTVLFNDTILYNIRYGR
                     PEASDAEIFDAARAAHIHDFIESLPKKYETAVGERGLKLSGGEKQRVAIARALLKNPR
                     ILIFDEATSALDSRAEKAIQAELERISQGRTTLVIAHRLSTVMDADQILVLAEGRIVE
                     RGSHAQLLEAKGEYARMWALQQQQEQAKELLESTTAA"
     gene            7555..8427
                     /locus_tag="VIG70_17105"
     CDS             7555..8427
                     /locus_tag="VIG70_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY8252314.1"
                     /translation="MGLRLKFNLVLLAVFVVGLGATGYLSYELLHRNARDEVLRNAGV
                     MMEAALSMRGYTVGHVRPLLPADPDKFLPESVPAFAATEIMTLLRKKYPDYAYKEAAL
                     NPTNPRNRAVEWETDIVNSFRTDPGLGEISGMRDTPTGRSLYLARPFQIKDQACLACH
                     TTWEMAPPAMVRIYGPANGFGWKLNEVIGAQIVSVPMGLPIRNANRAFVTFISSLALV
                     FAAVFVILNIMLTQLIVEPITELSQAADHISKGKVDIAEVPAKGKDEVAQLGQAFNRM
                     RRSLEKAMSLIDKG"
     gene            8436..9593
                     /locus_tag="VIG70_17110"
     CDS             8436..9593
                     /locus_tag="VIG70_17110"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8252315.1"
                     /translation="MNALPAGYALHEYRIDKVLGVGGFGLTYLAVDSNLNLKVALKEY
                     LPADIALRGANQSIGPRSPDTAETFGWGKQRFLDESRTVATFRHPNIVRVMRFFEANG
                     TAYMVMEFVEGSALGDWIKTRRPLSEADILPIAVQVLDGLEVVHKAGFLHRDIKPGNI
                     YIREDGTAVLLDFGSARQKASELTAVVSPGYAPFEQYHSHGKQGPWSDIYAFAGVLYW
                     MVTGGMPQEAPARIRADKMVSAVEGGDRTLFRTEFLAAIDWALTPFEDQRPQSIGEWR
                     EALLIGGPPPAKPVEKKPVETKLVEAKTEKVKPPKATTPPRLPPDILLRAQQRLAEYI
                     GPVATVVVKRAAAKARDEAELYLLLADEIEDKDEKKAFVRQAVSISGKPAS"
     gene            9689..10354
                     /locus_tag="VIG70_17115"
     CDS             9689..10354
                     /locus_tag="VIG70_17115"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY8252316.1"
                     /translation="MSIRPIKPQHETRTRILDAAEELFMLHGFEGTSMRHLTARAGVN
                     LAAVNYHFGSKHALIEAVFRRRLDPMNAERVAELARLEASNNLSPDSIIRAFVGPGLR
                     MIEDAKGGRNFIRLLGRTYTEPAKTVRALIGQMYAPAMERYKSALERALPQMPGDELV
                     WRMHFMFGTLAYTLAATDTVQLIAGCKPEDRYDARLLEDRLTAFLAAGLLAPLKSSIK
                     KAA"
     gene            10498..12843
                     /locus_tag="VIG70_17120"
     CDS             10498..12843
                     /locus_tag="VIG70_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY8252317.1"
                     /translation="MTNVVLGIALVAIAAVLNIPALRRMLISDRVLAIYRRILPDMSQ
                     TEKEAIDAGTVWWDADLFSGKPDWDKLLAVPEPRLSAEEQAFLDGPVEELCAMLNDWE
                     ITHERQDLPERVWQFIKDRGFLGMIIPKQYGGLGFSALAHSAVVMKLSTRSSTAAISV
                     MVPNSLGPGELLMHYGTEQQKNHYLPRLAKGLEVPCFALTSPEAGSDAAAIPDFGIVC
                     KGTWQGREVLGMRVTWDKRYITLGPVATLLGLAFKLYDPDKLLGSQTDLGITCALIPT
                     STPGVNIGRRHLPLNQAFQNGPNSGKDVFMPLDWIIGGPEYAGKGWMMLMGCLAAGRA
                     ISLPTSSVGGAKALTRFTGAYARVRSQFKTPIGKLEGVEEALGRIAAQCYMMDATRVM
                     TAGAVDLGEKPAVLSAIAKYHMTERARDCVNDGMDILGGKGICLGPNNWIGRGYQLSP
                     VGITVEGANILTRTLIIFGQGAIRCHPYVLREMRAAKEMQGAEAAREFDDAFTSHIGH
                     VLRNAVRTFVFGLTNSAFAPVPRNAASETKHYYRHVSRLSAAFAFLADISMLAMGGAL
                     KRKEKISGRLGDVLSMLYLVSATLKRYEDQGRIREDLPLVRWAVRDALYRAQRAIDGI
                     LSNFPVKALATLLRWSIFPLGMPYRPPLDSRNHECAKIALEPGAARDRLTAGMFVPRN
                     ESDATGRLEAAFLATIACEPIDEKLRKAVKKGKLVLQPGKDLGSLALEKDLITGDEYQ
                     QWSRKEALRKGVIKVDDFPQDFGRAALAAAQEGDTVKMKAA"
     gene            12845..14146
                     /locus_tag="VIG70_17125"
     CDS             12845..14146
                     /locus_tag="VIG70_17125"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY8252318.1"
                     /translation="MVADRSVYVVDGARTPFLKSKNRPGPFAAADLATQAGRSLLARQ
                     RFAPDELDEVILGCAAPSVDEVNIGRVAALRMGCGQKVPGWTVMRNCASGMQALDSGI
                     SNILAGRSSLVLAGGVDALSRAPLLYGDKMVLWFSDMASARTTGQKISLFGRLPVRAL
                     LAPVIGIMKGLTDPMVGLLMGQTAENVAHRFGITREEMDMFSVRSHERVIRAQDQGTL
                     APGGGEVEALYDAKGNVYTLDDGVRRDASIQNLAKLRPFFDRKYGNVTPGNSSQITDG
                     AAWVILTSEEALKKHDLKPIGRIVDSEWAGLDPATMGLGPVHAATPILKRHNLGLNDV
                     DYWEINEAFAAQVLGCLAAWADEKYCREELGLDKPLGELDEEKLNVDGGAIALGHPVG
                     ASGARIVLHLLKTLKRNNAKRGMASICIGGGLGGAMLVEAL"
     gene            14146..16098
                     /locus_tag="VIG70_17130"
     CDS             14146..16098
                     /locus_tag="VIG70_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8252319.1"
                     /translation="MERADAKVLAKHWRWEKDREGLAWLTFDKQGESTNTFSREALNE
                     LSLMLDEIAAARPKGLVIRSAKDSFIGGADIQEFANFKGPDDAVAFTRLGWDTFQKLR
                     DLPFPTTAMVNGFCMGGGMEMCLACTYIVALDDPKTRFAQPEVMVGVIPAWHAMEWLP
                     QRVGPAAAMDLLLTGKTIDAKRAKRIGLADEAVPLRIFENTARMITLEAPPRKKLSFV
                     NRLMLGPLKGIVASQARKQVARKAKREHYPVPYAIIDVWKNFGGNALIVPPNHPSSIP
                     TLIQHPTGRNLVRIFFLQDRLKALGKGVEFKARHVHVVGAGVMGGDIAAICAMRGLRV
                     TLQDTAPERIAPAVKRAAELFKRRLRDKARERDALDRLIPDVSGEGARQADVLIEAIF
                     ENLEAKRALFAKLESLAKPGAILATNTSSLKLADIATALKDPSRLVGIHFFNPVPLLQ
                     LVEVVSGDRTNPELAKKAAAFVRQIDKLPLPAKDSPGFLVNRVLGPYMLNAFRMLDAG
                     AKPETLDRAMEDFGMPMGPAELADTVGLDVCLAAGKALAKGATSVPEILANKVALGHL
                     GKKTGQGIYRYEDGKPVKGQPDAYDQALVDALIEPYLREAEAVVAEGIVADADLVDAG
                     LIFGTGFAPFRGGPLHYIKTRGRQAP"
     gene            16124..16468
                     /locus_tag="VIG70_17135"
     CDS             16124..16468
                     /locus_tag="VIG70_17135"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY8252320.1"
                     /translation="MPMPADANQNGDIFGGWIMAQVDVAGGTAAGRIARGRVATVAVK
                     EFVFKQPVQIGDLLSFYVDLVRVGRTSITVNVEVYAERRPADPKVVKVTEATLTYVAI
                     DRDGRPRPVPTA"
     gene            16470..17207
                     /locus_tag="VIG70_17140"
     CDS             16470..17207
                     /locus_tag="VIG70_17140"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8252321.1"
                     /translation="MLLDAPTLAAVAALAVVAAIMSGMSGFGAGIILTAALVPLIGVK
                     AVIPVLAVAGVVINAGRFWFYRTHLDRHMIALVLISSLPFLVLGTWLYSVLDARALSV
                     VLGLAVMASVPVRRVLHHKKITFGSTGVAVGSGVFGFAAGIASGAGVILVSLLLGAGL
                     ATPAVLATDAMISIVIDIAKAALFQRFDLLDTAAFFTGVVIGVASIPGSAIAAWLVSR
                     MGARLHVLFMEVLILFGGASMLWHAWR"
     gene            17212..18051
                     /locus_tag="VIG70_17145"
     CDS             17212..18051
                     /locus_tag="VIG70_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8252322.1"
                     /translation="MRNLKGRVAAVTGAASGLGRSMALAFAGEGMDVALGDVDEKNLS
                     RVEKEVSARGVRSIALRIDVSKAQDVEAFRDLALDRLGGVHLVCNNAGVSPLGAVWES
                     TPADWQWTLGVNLWGVIHGVRAFTPHLIAQNEGHIVNTASVSGLISPPGSGAYNVTKH
                     AVVTLSETLHHELRERNAAVGVSVLCPAYVPTGIVDSERNRPEDLPASERSPETLARQ
                     AMLRKAVASGKISADQVAQAVVAAVKEERFYILTHPRIKGAIQARMEDILEGRAPRNP
                     MTL"
     gene            18062..18850
                     /locus_tag="VIG70_17150"
     CDS             18062..18850
                     /locus_tag="VIG70_17150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8252323.1"
                     /translation="MTRTLVLLHGAASNSTRWAEFITHTNLRGEWKLVAPDLRGNGTA
                     AAPRTRLGMDVWCGDIVKLLDAERCERAVIGGHCFGANIALHFAARYPGRISALVLIE
                     PMPREAFVGSAAWLPFLRPLLYLTYGAARALNASGIYRRRLETLDLEEWDRPRRSGER
                     KLSMLASLGVDLGATPLAAYTRMLAATAEPWPDVAAIRVPVLALISSRSSMTDPARTR
                     RALAMLPDCDLLELDAVHWIPTEQPDAMRAAIGDWISRRLGSSP"
     gene            complement(18822..19487)
                     /locus_tag="VIG70_17155"
     CDS             complement(18822..19487)
                     /locus_tag="VIG70_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY8252324.1"
                     /translation="MLKLCGFHLSNYHNKLRIALLEKGVAFEEDPGCSPSQKDEFLAR
                     SPIGKVPFLELDNGRRLAESEVIAEYLEEAYPQKPLLPKDPFERAKVREIIKFMELHV
                     ELVARRLYGQVFWKRPVSDEVKQAAEKDLAKGIRAFKALVKFDPYIAGKELTLADCAA
                     FVHLPLVSLATKHGLGRDVLEDIPQVKPYLKMLGERPAFSRVNEDRKAAQAALMAKNR
                     AVG"
     gene            19508..20335
                     /locus_tag="VIG70_17160"
     CDS             19508..20335
                     /locus_tag="VIG70_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEY8252325.1"
                     /translation="MPDPKPEFITDLAADTSYGGYLGLERLLSAQQPRSSHHDEMLFI
                     IQHQTSELWMKLMIHELAAAIGHVKADRLDPCFKILARVKQIQRQLFEQWGVLETLTP
                     SEYAEFRNVLGPASGLQSHQFRAIEFLLGNKIANRVDVHRHDPQTTAYLEGLLRSPSL
                     YDEFLRYLARKGLPVPPGRVERDWSQPYEKHPGVTAVFKIVYQNTSSYWAEYDMCEKL
                     VDVEENFLLWRFRHVKTVERIIGFKRGTGGTAGVPFLRRTLETSLFPELIDVRTELG"
     gene            20328..21431
                     /locus_tag="VIG70_17165"
     CDS             20328..21431
                     /locus_tag="VIG70_17165"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8252326.1"
                     /translation="MDERWLRKEIFPKFSRVLQRNEIYLANHSLGRPPDRVADDVRAA
                     VDAWYRDLDGAWDSWLQHRERFRSLTAKLAGSPRADCIIPKTSAGQGLRAVLNSFATK
                     PRVVTSDGEFDSIDFILRVYREQARIELKITPWQELSAQGADLVVLSSVMFRTGEIVK
                     HLPKLVHDAHAAGALVLLDVYHHAGVLPLDLAALDADFAVGGSYKYTRGGPGACWLYV
                     RPGLVETLRTPDTGWFAKKDVFAYARPEPPEFGPGGDAWLESTPPVLACVQALAGLEL
                     TLALGVERLRKYSLEQKKILSDLIPGCEGAAESHGAFLTFRHPNAALLCLKLEQQGVR
                     VDARGEYLRLGPDILNTQAELEKAAAVLKSLLS"
     gene            complement(21428..22669)
                     /locus_tag="VIG70_17170"
     CDS             complement(21428..22669)
                     /locus_tag="VIG70_17170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8252327.1"
                     /translation="MKSADALVLGAGIVGVSVALHLQKRGRATVLVDRRGPAEETSFG
                     NAGLVQREGVYPYGFPHDFGALFRYSLNRTIDAHYHPSAIPRLAPFLWQYWKYSRPAR
                     HQAIARLYAKLIEHCITEHEALAADAGAQDLFRRTGWMKVFRSARERDLRFAEAERWR
                     GEFGLNYKAHDARTLQGLEPHLAPVLVGALHWTDPLAVVDPQGLALSYLRLFEKLGGR
                     FAQGNAASLENNNGWRVRTVDGPVGARDAVIALGPWSDVVTRALGYELPLAVKRGYHM
                     HYRPAGRAVLNHPILDTERGYFLAPMRRGIRLTTGAEFARRDAIRTPVQLGRAEPIAR
                     DLFPLAERLDNEAWMGSRPCTPDMMPVIGAAPRHPHLWFAFGHAHHGLTLGPVTGRLI
                     AEMITGEAPFIDPQPYRAERF"
     gene            complement(22671..23909)
                     /locus_tag="VIG70_17175"
     CDS             complement(22671..23909)
                     /locus_tag="VIG70_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8252328.1"
                     /translation="MNFDYTPKVQDLRKKVAAFMDEHVYPREHEWHAHVSGEQRWQPV
                     PLIEELKLKARTAGLWNLWRPKSHGGTLTNLEYAPLCEIMGRVTWAPEVFNCSAPDTG
                     NMETLLKYGTPEHVKEWCEPLLEGKIRSAFLMTEPNVASSDATNIQTRIAREKNEYVI
                     NGTKWWSSGAGDPRCKIYIVMGKTDPDNASRHSQQSMVLVPAGTPGVKILRHLAVFGY
                     DDAPHGHMEIELKGVRVPASNVLLGEGRGFEIAQGRLGPGRIHHCMRLIGQAERALEK
                     MCKRATERVAFGRPVSDQGVTRERIAESRIMIDAARYMVLNAAWKMDTVGNKVAKKEI
                     AMIKVFAPNVACQVIDWAMQVHGGGGVSDDFGLAHSYAHARTLRFADGPDEVHRNQIA
                     KLELGEQTSIAESLKKTVSR"
     gene            complement(23906..24958)
                     /locus_tag="VIG70_17180"
     CDS             complement(23906..24958)
                     /locus_tag="VIG70_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEY8252329.1"
                     /translation="MFEQFIGTKPVEERHRIDLAALEKFLNFKISSIEQFKGGQSNPT
                     YRISSPEGRFVLRRKPPGKLLPSAHAVEREYKVIKALHPTGFPVARPHVLCEDDSVIG
                     TAFYVMDYVEGRVLWDPALPGMTRGERAAIWDEQNRVISQLHLVNYRAVGLEDFGKPG
                     NYIGRQVERWSKQYRASETQRIEAMDSLIGWLPKNIPPEAGTSVVHGDFRLDNTIFHA
                     TEPRILAVLDWELSTLGDPLADFAYHCMSWHIPPDKFRGMGGLNLKELGIPGESEYVA
                     AYCRRTHRKSIDPSHWDFYLAYNLFRIAAICQGIAKRVLEGTAASTFAQEAASKTVPL
                     ADLGWQQVEKILRRAA"
     gene            24992..25411
                     /locus_tag="VIG70_17185"
     CDS             24992..25411
                     /locus_tag="VIG70_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8252330.1"
                     /translation="MRFSVSHSKGARFARRGLRSYFEYRDLGIKRATRGKVVAHVIRA
                     RVGKAPHGEWHAHDCKVQFVYVLKGWVLFEYEGVGRVMMKAGSCFYQPPRIRHREIRH
                     SRNLEMIEVVSPAAFRTYSASSAKPAARKSPGRRSRG"
     gene            complement(25341..25802)
                     /gene="dtd"
                     /locus_tag="VIG70_17190"
     CDS             complement(25341..25802)
                     /gene="dtd"
                     /locus_tag="VIG70_17190"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY8252331.1"
                     /translation="MIALIQRVTGADVKVAGEPVGAIGAGIVALIGVERGDTEAEALS
                     LAERVTNYRIFADAEGRMNLSVKDVRGEILAVPQFTLAADTNSGLRPSFSSAATPAEA
                     EKLFRRFVEKIQECSPRVATGRFGAHMQVSLVNDGPVTFWLQASPKTQNKS"
     gene            complement(25813..26034)
                     /locus_tag="VIG70_17195"
     CDS             complement(25813..26034)
                     /locus_tag="VIG70_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEY8252332.1"
                     /translation="MTDTPLTEEEKAQIRQRLHALEVEHHDLDDVIGRLVIDRSLDQL
                     QLQRLKKRKLLLKDQIQRLRARLIPDIIA"
     gene            26101..27030
                     /locus_tag="VIG70_17200"
     CDS             26101..27030
                     /locus_tag="VIG70_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252333.1"
                     /translation="MRRSDRDVADSIQTTDPAAVGDEVIRLARRLFPDASAPTLERAF
                     TDAARMYRGEHRDYLPCDTEYHDIQHILDVTLAMARLMDGYQRGKRNGSPELNKELFL
                     AGALAALFHDFGYLRRRNDHTHRYGAEYTLTHVSRGAAFLRGYARDLGVGERLAQAAS
                     TLVHFTGYERPIESIRLNDVALRNLGLMLGTADIIAQMSDRCYLEKCRDRLYPEFVLG
                     RIPNFSSGRDLVMKTPLFYEGACKRLDDKLGRSYDYAACHFGGPNLYLDAMRKNAEYA
                     LSISESGQFTLRRNPPKTLPPDVEPYPKDLIGS"
     gene            complement(27016..27987)
                     /locus_tag="VIG70_17205"
     CDS             complement(27016..27987)
                     /locus_tag="VIG70_17205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEY8252334.1"
                     /translation="MRTLALALSVCLAALPAAAQDKPEDTLGAVVGVSAKIQANARSA
                     ETLGLQRNGTGALVRKDYVLTIGYLVIEAEAIQVTGADGRTMPATVAGYDHASGFALL
                     KIVGNLGAKPLPLGDSVALGEREPAMVASAAARDSPTLVYVVSRRAFSGNWEYLLDSA
                     IYTYPAVMDWSGAPLIGAKGELLGIGSLIVPDAGGPGTQSPGNMFVPVHLLKPILEDL
                     IAKGRRNGPARPWLGLNTDEYRGRLFVGRVSPDGPAARAGLKSGDIVLAVGADEVTTL
                     AEFYRKVWGRGAAGSNITLKVLQGASVKDLTVRSIDRIEYFRPSRTY"
     gene            28020..28769
                     /locus_tag="VIG70_17210"
     CDS             28020..28769
                     /locus_tag="VIG70_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252335.1"
                     /translation="MSAFWLNELARTAVLFLTAYGLGVWVKRTGIRVNYTRKIMHFVI
                     LLFPMALAAVFPYEASLFTVVASGLVFIASLGLFTRPLRERSSIVATAFGSFDRPEDR
                     PFTLLWLSTQLLATYAVLVVMLLWLGRYEKATLIYITVLVAGIGDGLAEPVGVRFGRH
                     TYSTRALFTDRRYTRSLEGSACVLASGFLAVLLLRPQLDEMQFVLALAIIPLAMTLAE
                     ARSPHTWDGPFLYLAGGASTVATLELSRLLQ"
     gene            28800..29375
                     /locus_tag="VIG70_17215"
     CDS             28800..29375
                     /locus_tag="VIG70_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252336.1"
                     /translation="MFLTALAASLALGAGLLAAASYSEGTLIALRTAAQAIGWLFILA
                     TVVGLTGLRQASFGRLGAATVAFLSGVTMLYVAYFQWDQAVEAPIRHAAAETLSAREP
                     AYRVTLTSLEAVTPVAMKEVPPENKPVMKNAGDACASLKGVESLQCSRCTAKFGVAWI
                     VCQESARLEYCRGREDDDAACPSAIPFSPPG"
     gene            complement(29350..29724)
                     /locus_tag="VIG70_17220"
     CDS             complement(29350..29724)
                     /locus_tag="VIG70_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252337.1"
                     /translation="MDLTPGERAVLAQIRVLYSYAAARDQQLKALRTQWPPIHCQAYE
                     RAYAGLLAKDLIQDLSAQIFRITDAGLTAIGVVTVAQPRPQPRPVPVVRPAAPVTPRV
                     AAKPRNLLSRFMSGFSRAAKTE"
     gene            29808..30644
                     /locus_tag="VIG70_17225"
     CDS             29808..30644
                     /locus_tag="VIG70_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY8252338.1"
                     /translation="MKSKKRSSRHMRLLSWNVQWCRGLDGRVDPKRIADEAKRIADPD
                     VFCLQEIARYFPEMEGSAGEDQVEVLAREFPGYEAFFAPGVDLPGKRGRRRFGNLVLT
                     RLAVGRVLRHQLPWPSASAVRSMPRAAVEVVVEAPFGLLRVMTTHLEYYSKDHRTAQI
                     ERLRELHAEACAEHLPVDEPGSYESHTRAKSALVCGDFNLKPDDPGHRQMLDAGFVDA
                     WLALNPGKPRQATFHLHDGEPPYCCDYMFVTPDLVPRLRALRIETETQASDHQPVILD
                     LA"
     gene            30577..31476
                     /locus_tag="VIG70_17230"
     CDS             30577..31476
                     /locus_tag="VIG70_17230"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY8252339.1"
                     /translation="MRYASKPRRRLRITSRSFSILLEVRKLADALGAEIGSLDLSLPL
                     TEQTFREVREAWLEHLVIRIRGQKLSDPELLAFSRNFGELDPPGQNPLGRPFLADHPE
                     MNVISNIKHDGVPIGGLGDGEAIWHADMTYVERPPMAAILYAIEIPPAGGDTYWANMV
                     AAYETLPEELKRRIEDLEAIHDATYNSAGTMRKGYGEVTDPRTAPGARHPLVRTHPET
                     GRRSLFLGRRRNSYIVGLDLSESENLLDTLWAHATQPRFTFRQEWRVGDVLVWDNRCT
                     LHRRDAFDPRARRLLHRTQVRAA"
     gene            complement(31463..31873)
                     /locus_tag="VIG70_17235"
     CDS             complement(31463..31873)
                     /locus_tag="VIG70_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEY8252340.1"
                     /translation="MVASGAMFDVSFSFRRPADLGVKNGRLAPCKRSPNCVSSQADPS
                     DREHHIAPLRVSMEEVRKAVEALPRTRIVLAHSNYLYAEFRSKLLGFVDDVEFFFDGA
                     VVQVRSASRLGRRDFGVNRARIEKIRRLLEPRRP"
     gene            31900..32175
                     /locus_tag="VIG70_17240"
     CDS             31900..32175
                     /locus_tag="VIG70_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252341.1"
                     /translation="MTPQQLAELLLGIARAQAAIIQGLENEMAGVRSGRIIPALQNVA
                     HLRDHPQPTLTDLPVRVLLGTLGRQVPDAAGLVRDLERLLSKPAGSA"
     gene            complement(32150..32548)
                     /locus_tag="VIG70_17245"
     CDS             complement(32150..32548)
                     /locus_tag="VIG70_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEY8252342.1"
                     /translation="MIFTARHAIGGPARGQALVAHDGFSARYDLDRIRGVFSRPAHKL
                     AGQSYVGKVLVLDTAKGGVATAWMLHEMASRGVVPLAIVLNSVNPILAQGAAFGGVTL
                     LAGFDGDITARVPHGATVEIDPARRTLRVC"
     gene            complement(32545..33756)
                     /locus_tag="VIG70_17250"
     CDS             complement(32545..33756)
                     /locus_tag="VIG70_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HEY8252343.1"
                     /translation="MRLNDLERAMLAGELGAAAKAAIEHQVKVGEFFGAADFVPVTQA
                     HIMADTESLGEAGVRWVESLGAQGGRVRVPTITDPRGTDFAKAGVLKHPAWIVDLERR
                     TVDAFKKLGVLMTDTCINYQTIQAPARGERVAFGDTGVVIYANSVCGARSNYEGGPSA
                     VAAGLTGRTPRYGFHLDECRRATLRVQVSFQPQTLNDWGALGGLVGRLAGNYWAVPYV
                     EGLERVPGSDELKHFGAAMASFGSIALFHLAGITPEAADSPLKVSHRIGEGDVKALQA
                     VYAGEDEIDVVVFSAPQLSLYELRSLAALCDGRRFARPLLAVTSPQVKPDADRLGLTK
                     RIEDAGGHVLSGMCFYQSYAREIAEANGWKRLATNSVKLVNILGGYGYVPLLASMERC
                     VQAAESGRLPR"
     gene            complement(33758..34564)
                     /locus_tag="VIG70_17255"
     CDS             complement(33758..34564)
                     /locus_tag="VIG70_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY8252344.1"
                     /translation="MDYSAYKDLRIHRIEPGILEIVMGEEGKLAVATVRAHAEMARVW
                     LDVDRDPDTRVAILRGAGKGFSAGGDMAMVDEMTRDFAARARVWREARDLVYNMLNCS
                     KLIVSAMHGPAVGAGLAAGLMADISIAARNARLIDGHTRLGVAAGDHAAIIWPLLCGM
                     AKAKYYLLTCDTISGEEAERLGLVSLTCEESQLREKALEVARKLAAGPQTALRWTKYS
                     LNNWLRLAGPSFDASLAFEILGFTGPEVKEGLASLREKREPKFPAEPKDF"
     gene            34586..35449
                     /locus_tag="VIG70_17260"
     CDS             34586..35449
                     /locus_tag="VIG70_17260"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEY8252345.1"
                     /translation="MRTPDGSFVDLPGFPYAAHYLEWRGLRTHYVDEGKGDRVYLCLH
                     GEPTWCYLYRRMIPALVASGGRVVAPDFVGFGRSDKPGDEAGYTFDFHRGFLLEFIEQ
                     KQLRNITLVVQDWGGLLGLTLPMEMPERFERLLVMNTTLGTGDVALPEGFVAWRAYVR
                     KNPDLGCGKLLARTCPHLTAAEAAAYDAPFPDARSKAGVRRFPELVPERYDDPGAAIS
                     RKARDWLSTSWKGESFMAIGMKDPVLGEPVMRALQRSIRNCREPYLVAQGGHFLQEWG
                     EEVTQWFLSKT"
     gene            35428..36387
                     /locus_tag="VIG70_17265"
     CDS             35428..36387
                     /locus_tag="VIG70_17265"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HEY8252346.1"
                     /translation="MVSFEDIAAAHERIKPQAKRTPVLTSATVDALTGARVFFKCENF
                     QRMGAFKFRGAFNALSQLGPEQRQRGVVAFSSGNHAQAVALSGKILGIKTVIVMPLDA
                     PRVKLEATRGYGAEVVQYAKGQPRDELAGKLATERGLTLVPPFDHPHIIAGQGTAAKE
                     LVEDAGPLDVLLVPCGGGGLLSGCAIAAKHLAPKCRVLGVEPEAGNDASQSFNQKRLV
                     TIAIPDTIADGARTPSLGKLTFEIVMRTVDDMLTVTDPELTRAMFFLWERLKIVVEPT
                     GALAFASLFTGKFSGKGRRIGVVLSGGNVDLRWAAAQLDKNQG"
     gene            complement(36328..38319)
                     /locus_tag="VIG70_17270"
     CDS             complement(36328..38319)
                     /locus_tag="VIG70_17270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEY8252347.1"
                     /translation="MGLLTWEGSRHRPGGVWAVLIKAGSVAAALYVLWIGALAQITGN
                     AALWLSRFALEGPLVAFATWARAFTAYFHLDISIFIAITFPIAFLTTTARRGRSRLAW
                     TDVALAAASLAVALYYILFNDRFLHWNRGFSQPNTADLVAGFALLALVLELCRRSVGW
                     GLTSLVSILLSMAVLGRWLPGALHHDNFGITYFIEMMTIMENGVFGAPLEVAATYAFL
                     FVLFGSFYEKSGGGQLFFDLASAVTGRMRGGAAKACVTASGLYGSISGSPTADVATTG
                     PLTIPIMKKMGVPAARAGAIEATASSGGAMLPPVMGAVAFIMSDITNISYAAICWASV
                     VPALLYYYSIYLLVHNEAVRHNEPCLPEADIVPLRRALASGWRHLLPLGALIALLVAG
                     YTPVYVAAGATAAVIVLSWFHRPTAIGPRRFVECCTDTILQLVPLVGAVAAAGVVIGA
                     IEIAALAGKFTLLINMVSGGLLIPTLLLSAVFLVLLGMGMPTPAVYIMGAALLAPVLR
                     GVFNLPEMQVHLFMLYFACLSAITPPVAVANFAAGAIAGVNPMALGPYTVKLAIGGFI
                     LPFYFLFNPGLNLQGEVLYILECVVFAIAMCTFASFALQGYFGLRRIAWPLRFLLFGC
                     AIAAVSPRFDITLAATLIGASTLVLVQLRGRPTKVDVSA"
     gene            complement(38326..39330)
                     /locus_tag="VIG70_17275"
     CDS             complement(38326..39330)
                     /locus_tag="VIG70_17275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY8252348.1"
                     /translation="MKAVLMALAAALAAPALADFNDKNPINATMTGATPSGYPRTMVE
                     GLNAVVRDAYPGSAVSFKPNSPGGGVLAVAEGQADFTATATGTEIRLANEGTAPFTKP
                     LKGKFSYVMQLYDNQFIHFLMTKEWADANGVKSWADIAAKKPRMRLAINRTDNPQTTI
                     GGPYEVMKAHGFTIQDIEKWGGSYVLGNSQIGLDAIVDGKADVFMNARNLGDSLVKDV
                     SGKRALLWIDGDRATIQKAADTFNFKADMVEKGTYPFMEKDYPTVRMWISIVAGNHVS
                     DEVVYKYVKAIAENEKRVQDIGGSLKTAFATQKMSVNPANLPYHPGALRYYKEKGLVK
                     "
     gene            complement(39327..39620)
                     /locus_tag="VIG70_17280"
     CDS             complement(39327..39620)
                     /locus_tag="VIG70_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252349.1"
                     /translation="MAQFRVEFLLDQATGKYLAELYNPDNQQELMVRTEAIYASQAAA
                     VLGLVQLFKDAILKFPEPGKRARTQPKLKRRRSDRKAKPKAKKAKKAKRRKHG"
     gene            39655..40626
                     /locus_tag="VIG70_17285"
     CDS             39655..40626
                     /locus_tag="VIG70_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY8252350.1"
                     /translation="MRAVEITKPGGPEVLKPADRPKPVPKENEILVKVAAAGVNRPDV
                     LQRSGNYPVPPGASDLPGLEIAGEVVGGSTKHFKPGDKVCALVAGGGYAEYCAVPEVQ
                     ALPVPKGLTPVQAASLPETFFTVWSNVYDRAKLAPGESLLVQGGSSGIGVTAIQMAAA
                     LGNRVFATAGSDEKCAACLRLGAAAAFNYRTQDFEKEILGATGGKGVNVILDMVGGDY
                     VPKELKCLAEDGRLVFIAYLRGPKAEVNIDTVMRRRLTISGSTLRPRPIEFKGAIAHS
                     LHEKIWPLIESGRIKPEIYKTFPLEQAAEAHKLMESSQHIGKIVLTV"
     gene            complement(40616..41524)
                     /gene="gcvA"
                     /locus_tag="VIG70_17290"
     CDS             complement(40616..41524)
                     /gene="gcvA"
                     /locus_tag="VIG70_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEY8252351.1"
                     /translation="MRNRNALPALDLLVGFEAAARHLSFTKAGEELHLTQSAVSRQIK
                     ELEDQLGVVLFQRRHRALALTEAGHQFYAAAAQALSTMRTATDRLRAHAGKKALSVTT
                     THSFAALWLIPRLAGFTRDHPGVDVRITADTKVQDLERDGLDLALRHGPASLAGPNAI
                     RLFGEKVFPVCSPKLLRKKPLAKPADLRNHVLLQYDDPEVRHPWLHWKTWLEVERIAD
                     LRPAGTLSFSGYEQIIPAAVAGHGVALGRSPLVKDLIAEGALVAPFKSQADPARAYFA
                     IVSRNAAGRPEVAAFLEWLKTESAKR"
     gene            41603..41830
                     /locus_tag="VIG70_17295"
     CDS             41603..41830
                     /locus_tag="VIG70_17295"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEY8252352.1"
                     /translation="MRIELRTGEFQLQRGQTLKVVDGAGSTICASQAAVWITEENRPR
                     DIVVEPGGCYRLRERGVAVIEALGKASVSFA"
     gene            41946..42113
                     /locus_tag="VIG70_17300"
     CDS             41946..42113
                     /locus_tag="VIG70_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252353.1"
                     /translation="MKLTYEAILNDPGLLERLEKQAHRERAAAVHQLIVEPIKRVFAD
                     HAARPHLARQG"
     gene            42079..42480
                     /locus_tag="VIG70_17305"
     CDS             42079..42480
                     /locus_tag="VIG70_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEY8252354.1"
                     /translation="MPLVRISLAKGKPEAYRRKVGDAVHRALVETVGVPPLDRFQLLT
                     EHEPGDLVYDSNYLGIARSSDLVIVQITLSAGRTLAQKRALYRRIAANLAAAVGLRPQ
                     DAWINLVEVAKENWSFGNGAASYAPAEAQAA"
     gene            42477..42680
                     /locus_tag="VIG70_17310"
     CDS             42477..42680
                     /locus_tag="VIG70_17310"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEY8252355.1"
                     /translation="MSPAAQVLEQIDEVLQSLGANKSNLLTARVLLSDAELFPDYDNA
                     WNKWLDALRRPLRVWRPAEENHV"
     gene            42673..43023
                     /locus_tag="VIG70_17315"
     CDS             42673..43023
                     /locus_tag="VIG70_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8252356.1"
                     /translation="MYDHIGLRVKDLKAAVRFYQSALEPLGHMLDDSGVGLGPRGQPG
                     LWLHADGQPGGGAHVAFRAAERQAVDRFYAAGLKAGGRDNGKPGVRTDYSPTYYAAFL
                     IDPDGNNVEAVCVK"
     gene            43033..43908
                     /locus_tag="VIG70_17320"
     CDS             43033..43908
                     /locus_tag="VIG70_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8252357.1"
                     /translation="MFKPDLLKGKRILITGGGTGLGKEIAAKYLELGAELWICGRRGG
                     VLDQTARELVSRHPGSIKTHAIDIRDAAAVDAMVQRIWDEAGPLTGLVNNAAGNFISP
                     TKDLTPNGFNAIANTVFHGTFYVTHAVGKRWIAGGNKGSVVSILVTWVWTGSPYVVPS
                     AMSKAGLHIMTKSLAVEWGKHGIRLNAIAPGPFPTEGATKRLRPGGDFGESTKVNPMR
                     RVGEMSELQNLATFLMADGCEWLSGETIAIDGAGYLATGAYFTELDKLSDADWERARA
                     MIKAQNDKDRAGRSA"
BASE COUNT         7435 a        14708 c        14542 g         7243 t
ORIGIN      
        1 gggctggcgt ccttgacgtc cctgaggccg cagagtccca tggtgacgtc gagctcggcg
       61 cggatgatct cgagcgcgcg cgtcacgccc gcctcgccca gcgcgcccag gccgtaggcg
      121 taggcgcgcc cgatcatcgt cgccttggcg cccagcgcga gcgccttgag cacgtcctgg
      181 ccgctcctga tcccgccatc gacccagact tcaatcttgt tgtttactac ttcagcgatc
      241 tctggaagcg cgcggatcga cgacaccgca ccatcgagct ggcggccgcc gtggttcgac
      301 accacgatcg catcggcgcc cgatgatgcg gcgatcttcg cgtcctcggc gtcgaggatc
      361 cccttgagga tcagcttccc gccccagagg ttcttcaccc actcgacgtc cttccacgag
      421 agcgtgggat cgaactggcc ggcgatccac tgcgagagcg tggtgaggtt cttggcgtcc
      481 gggatgcggc cctcgaggtt gccgaaggat tttctttttc ccctgagcac gttcagcgcc
      541 cagccgggtt tgcgcaacac atcgaaaacc gtggcggcgg tcaggcgcgg cggaacggcc
      601 agtccgttct tgaggtcgcg atggcgctgg ccctggatct gcaggtcgag ggtcagaacg
      661 agggcggagc agcgtgcggc cttggcgcgc tcgatgagag acgcggcgaa gccgcggtcg
      721 cgcatcacgt agagctggaa ccagaacggc ttgccggtgc cggcggcgac gtcctcgatc
      781 gagcagatcg acatggtcga cagcgtgtac ggcacgccga agcgcgcggc ggcgcgcgcg
      841 gcgaggatct cgccgtcggc ccagttcagg ccggtgaggc cgacgggcgc gagcgccacc
      901 ggcatcgcga cgtcgtgccc ggccatggtg gtgcgcgtcg agcgccggtc gacgtcgatg
      961 ccgacgcgct ggcgcagcga gagcaattcg aggtcggcgc ggttcgcgcg cagcgtcgct
     1021 tcgctatagg agccgcggtc gacgtagtcg aagattgcgc gcgcaacccg cttcctcgag
     1081 agctcgcgca gatcgtcgat gcacgtcacc accggcatgg cggcaaattc tacacgctgg
     1141 aaagcgcaac cttcctcgta gacgctcgcc cacgttcgga atttgcgcgt aaggcattga
     1201 cgctgtgacc tcctctacgc cgacgaactt tcgcgaaacg tcggatggag cagcgcgcct
     1261 gtcccaaagg gagatcagtt ggcgatgggt tgctgttccg cgtatcgcca gcgggtaagg
     1321 cgtcgtgggc gatgcgttat cgattcaatg gtcagccgcg atactttccg atcgggcgct
     1381 atccggccgt ctcgctcgag gctgcgcgca tcgcggcgaa gaaggcgtgg gcaaagatct
     1441 acgacggtgc cgctgatggc gtggcggaaa acgatttgct tgacccgacc attggggcat
     1501 aaccgcactt tcatcggagc acgtcagcgc cgccgtcccg ctagaacccg cgcagcctga
     1561 aacgccagaa cgcgcagacc gcaaatatgg gataggagat gccccgttgg gggcgtatgg
     1621 tggccagata ggggtaatgg gcatcgctcc gcccgcccca gggaaataga gaagccgggg
     1681 ggcttttatg cttcgcaacc ttttgctggc cgggctagcg gtgcttgtgt tcgtgtcctg
     1741 tgggggaggt gatgatgaag aagaaccgcc tccaaacagg ggccatctca ccataggaaa
     1801 cccctcggcc gatgctgtca ccgtcgcacc atcggaaact accatcgagt tgtccggaac
     1861 cgctttcaac gcatcctttt tcttcaactg cttttttggt acgaaccacg ttgccgacat
     1921 aggagtttct gtcacgtgga acaatgcggc gggaggaagc ggtacggcat cccagtcggt
     1981 cggctgctgc tccattaccg gtacgggggg taagggatgg ctttgcgccc tccatggcgg
     2041 cagtggtacc gtgggcgacc ataattggag cgcctcgata ccgattatcc ccggcacaaa
     2101 cgtcgtcacg atgacagcca gcgattcaag cggcaacgtc gggcgcgata cgatcaccat
     2161 cactcgctaa ctatcaggcc accgtatctg cctgcttgcc gaattcgtcg gtgtatcgcc
     2221 attggaggtc accgcgctga gcgctgagag gtgctcgttg agccgctcgg tgtccggatc
     2281 tcgagcgatc cctcgacctt caccgcgtca ttgccgaagc gctcgcgcgc ctgccacctt
     2341 gattgacttt gcacgctgta aaatggcgca cgcatgtctg ccaacgacga gcacaagagc
     2401 ccgatccagg tcatcgaccg catgatgcgg ctgctcgacg tgctcgcgca gcatccggag
     2461 ccgctcggcc tgaagcagat ctcgcagtac accggcctgc atccgtcgac cgcgcaccgc
     2521 atcctctcct cgatgtccgc cgaccgcatc gtcgaccgcg tcgagcccgg cagctatcgc
     2581 ctgggcatgc ggctgctcga gctcggcaac ctggtcaagt cgcgcatcag cgtgcgcgag
     2641 ctcgcgctgc cgatgatgcg cgagctgcac tcgcagaccg gcgagaccgc gaacctctcg
     2701 gtgcgccacg gcgacgagat cgtctacgtc gagcgcacct cctcgggtcg ctccgcgatg
     2761 cgcgtggtgc acgtcatcgg cgcgcgcgcg ccgctgcacg tcaccgctgc gggcaagctg
     2821 tttctcctcg aggaaggctt cgcgcggctg cgcgactacg cgaagcgcac cgggctcgcc
     2881 gcgcacacca agaacacgct cgccagcctg acgctcctcg agcgcgagct cgagcgcatc
     2941 cagcgccagg gctgggccac cgacaacgag gaagccgaaa tcggcgtgcg ctgcgtcgcc
     3001 gccggaatcc gcgacgacgg cggcaacctc gtcgccgccc tgtcgctctc cacgccggcc
     3061 gaccggatga aggtgcagtg ggggccgctc gtcaaggaag ccgccgagcg catctcgcgc
     3121 accatcggcc accgcgagcc ggtggcgcga atcggggcgc attgatgcgg ggctgggttg
     3181 cctgcacagc attcgcggca actcttttct gtacccaggc cgccgggcag ccacctgagg
     3241 ttgaaactcc gcacctgggg ccagaaatac ccaagcctgc gccgatcggc gtgatccccc
     3301 ttccggcaga gccgccgccg acgccgcctc gggcggttca aaaaccaaag ccgctcgcag
     3361 caccccccgc tcccgcgccg aaaccgccgc cgctcacagt tgagaagcta gcgccgccac
     3421 cgagcgccgt tcagaaacca gctccaccgg cagcggcggt tgagaagccg gctgcggcgg
     3481 tcgaaccggc gcggccgcag gcggagtgcg agatcaagcc tgtgatgagc gacgacgatc
     3541 tgcgcgcctg cggcgtgcgg cgctgaattc ggggtcaggg tcgcaacttg tttttgcgta
     3601 cgcgaaaacg agtttcgacc ctgaccccga attgacggcg ctagctcaag ccgtgcagcg
     3661 cgaatagccc gcggcggtcg gtccacactc tcgcgctcct gaaccccgct cttgcggcga
     3721 gctcgcggaa cccgtcgagc gagtacttgt aggaattctc ggtatggatg gtttcgccgg
     3781 ccgcgaaatg ccagcggtgg ctgcccagcc ggacgcgctg cgccgcgagc gcgacgaggt
     3841 gcatctcgat cctgccctgc gccgggttgt agaaggcgta gtggcgccag cggcgcgggt
     3901 cgaagtcggc gccgagctcg cggttgatgc gcgcaagcag gttcaggttg aacgccgcgg
     3961 tcacgccggc ggcgtcgttg taggcggcgt gcagcagcgc gggatccttc ttcaggtcga
     4021 cgccgaccag catgcggacc gcgacgccgc gcgacatgga aaggaacgcg tgcgcttcct
     4081 cgggcgcgag attgccgatg gtcgagcccg ggaagtagac cacgctcggc gtgccgcgcc
     4141 gcacgcggat gtcgagcggc cgcgagaagt cgccgaccag cggcgcgaca cgcagccagg
     4201 gaaactcgcg cgcgaggccg cgcgcggccg cgagcagcgc gtcctccgag atgtcgatcg
     4261 gcacgtagac cgagggccgc agcgcgcgga tcagcatgcg gctcttgacg ccctcgccgc
     4321 tgccgtattc gaccagctgg cagccgcggc cggcgaagcg cacgatcgca ggaagatggc
     4381 gccgcgtaag cgcgagctcg gcgcgcgtca ggtagtactc gggaaggcgg cagatgcgag
     4441 cgaacaggcg gctgcccgcg gcatcgtaga agtacttcgg agggagggac ttctgcggga
     4501 gcgaaagccc cgcgacgacg tcgtttcgaa agctcgctag ctcgagcggc cggttacggc
     4561 cccgctctcg agccacttct tgatgcggtt cgcgtcgccg atccgcgagt gcttgcccca
     4621 cgagtcgagc agcaccacga tcaggtcctt ctcggcgagg cgcacgtgca tcacgaggca
     4681 gcgtcccgcc tcgctgatgt agccggtttt ggagagctcg atgtcccagt acttcgagcg
     4741 taccagaccg ttggtgttgc cgtaggcgag cggtcgcttc gaagcgcgga tggtggcgcg
     4801 gtctttggtc gagtattcgc ggatcagcgg atagtcgtgc gccgcgcgca ccagcttcgc
     4861 gaggtcgctt gcggtcgaga cattcgccgg cgacaggccg gtcggctcga cgaagcggct
     4921 ctcgtgcatc tcgatcaccg cggccttcgc gttcatcgcg tcgacgaaga cttccagccc
     4981 gccgggatag ctgtggccga gcgccgcggc ggcgcgattc tcggaggaca tgagcgcgag
     5041 cagcaggagg tcgccgcgcg agatcgacag cccggtgcgc agccgggagc gcgcgccctt
     5101 catcgccacc gcgtcctcgc ggctaagcac gatctgctct tcgaggtcga ggccgctgtc
     5161 gaggatcacc atcgcggtca tcagcttggt gatcgacgcg atcggcacca ccgcatccgc
     5221 gttcttgccg aagacgactt cgccggtctc ggcatcctgc accagcgccg acgacgagcg
     5281 cagcgcgatc ttgcggacgt cctgcgcctg cgcgaagccg acgctcagaa acagcacgag
     5341 gacgagagcg cacatggcga caatcccctc gagcggatgc agtttgcgtt gcattgccgg
     5401 atcttctccc tggacctgcg agttctcttg tatttaccta tgccctgatt ggcacatgga
     5461 ttcgtcgcag tctagcctac gccctcgaaa aagagcaagg aaaacaagtg ttaggaagtg
     5521 atttagagaa tcggtgtgaa atgtttcact gtcgggcgcc ggcgtcgccg gctccgattt
     5581 gccctaggcg gctgtagtgc tctcgaggag ctccttcgct tgctcctgct gttgctgcag
     5641 cgcccacatc cgggcgtatt cgcctttcgc ctcaagcaat tgggcgtgcg atccgcgttc
     5701 cacgatgcgg ccctcggcga gcaccaggat ctggtcggcg tccatcaccg tcgagagccg
     5761 gtgcgcgatg accagggtgg tgcggccctg cgagatgcgc tcgagctcgg cctggatcgc
     5821 cttctcggcg cgcgagtcga gcgcagaagt ggcctcgtcg aagatcagga tgcgcggatt
     5881 cttcaacagc gcgcgcgcga tcgccacgcg ctgcttctcg ccgccggaga gcttcagccc
     5941 gcgctcgccg accgcggtct cgtacttctt cggcagcgac tcgatgaagt cgtggatgtg
     6001 cgcggcgcgc gcggcgtcga agatttccgc gtcgctcgcc tcgggccggc cgtagcggat
     6061 gttgtagagg atcgtgtcgt tgaagagcac cgtgtcctgc ggcacgatgc cgatcgccgc
     6121 gcgcaggctc gcctgcctca cctccctcgt gtcctttcca ttcacgagaa tcgagccgcc
     6181 cgacacgtcg tagaagcggt agagcaggcg cgcgagcgtc gacttgcccg atccggagtg
     6241 cccgaccacc gcgacgcgat ggcccgcggg gatggtgaag ctcacgtcga acaggatctg
     6301 ccgcgcctct tcgtaggaaa aatccacccg ggaaaattcc accgtcgctg ccccgtgtgg
     6361 cacgtcgggc gcgccgggac ggtcctcgac ctcgcggtgc gccgacagca gccggaacat
     6421 gcggtccatg tcgagcagcg cctgcttgat ctcgcggtac accatgccga ggaaattgag
     6481 cgggatgtag agctggatca ggagcccgtt caccagcacc aggtcgccga gcgtgaagct
     6541 cctggcagcg acgccttcgg cagcgagcag catcagcgca gtcaccgcca gggcgatgat
     6601 caggctctgg ccgatgttca gcacgccgag cgaggcctcg ttccgggtcg cggcgtcctc
     6661 gtacttctgc agctgctcgt cgtagcgccg cgcctcgaat tcctcgttgc cgaagtactt
     6721 cacggtctcg tagttgagca ggctgtcgat cgcgcgcgtg ttcgcccgcg aatcgagctc
     6781 gttggcgcgg cggcggatct ccatgcgcca ctcggtgatc gtcacggtga acgcgatgta
     6841 caccgtgacc gcgccgaagg tgatcgcggc gaagcgccag tcgaacttcg cgatcagcac
     6901 cgcggcgacc agcgcaaatt ccaggatcac gggaaagatg gagaagatga ggtagctgag
     6961 gagcgtcgag atcccgcgcg tgccgcgctc gatgtcgcgc gtcatgccgc cggtctggcg
     7021 atcgaggtgg aaacgcagac tcaggctgtg gaggtggcgg aacacgccga gcgcgacgcg
     7081 ccggatcgcg cgctgcgtga cgcggacgaa gagcacgtcg cgcagctcct ggaacagcgt
     7141 ggtcgagaag cgcagcacgc cgtagatcgc gagcagcgcg accggcacgg cgacgagctg
     7201 caggctcggg tcgagattgt cgaccacctc cttcatcacc agcggcacgc cgacattggc
     7261 gagcttggcc gtcaccaggc agacgagcgc cacgacgacg cgccacttgt actcccagag
     7321 atagggcgcg agcgtcccga gcgccttcca ttcgttgcct ctcaatcgct gcgtcggcgc
     7381 tgcgctgtgt gccatttccc gattatgtca gcgagatctg cggaatccgt cacacgccgt
     7441 cacctcccgg ttacctcccc gaaacgccgt ggaaagaact gtcagggccg gccggtgtaa
     7501 gctcatccgc acggacgcga cgaacgtata aagcgcggtc gaagggagaa aaacatgggt
     7561 ctgcgcctta aattcaacct ggtgctgctc gccgtgttcg tggtcgggct cggcgccacc
     7621 ggatacctct cgtacgagct cctgcaccgc aacgcgcgcg acgaagtgct gcgcaacgcg
     7681 ggcgtgatga tggaagcggc gctttccatg cgcggctaca ccgtcggcca tgtgcggccg
     7741 ctcctgcccg ccgacccgga caagttcctg cccgagagcg tgccggcgtt cgccgccacc
     7801 gagatcatga cgctgctgcg caagaagtac cccgactacg cctacaagga agcggcgctc
     7861 aaccccacga acccgcgcaa tcgcgccgtc gagtgggaaa ccgacatcgt gaactcgttc
     7921 cgcaccgacc cggggctcgg cgagatctcg ggaatgcgcg atacgccgac cgggcgctcg
     7981 ctctacctcg cgcgcccgtt ccagatcaag gaccaggcct gcctcgcctg ccacaccacc
     8041 tgggagatgg cgccgccggc catggtcagg atctacgggc cggccaacgg cttcggctgg
     8101 aagctgaacg aggtgatcgg cgcgcagatc gtctcggtgc cgatgggcct gccgatccgc
     8161 aacgccaacc gcgcgttcgt caccttcatc agctcgctcg cgctggtgtt cgcggcggtg
     8221 tttgtaattt tgaacatcat gctgacgcag ctcatcgtcg agccgattac cgagctctcg
     8281 caggcggccg accacatctc gaagggcaag gtcgacatcg cggaggtgcc ggcgaagggc
     8341 aaggacgagg tggcccagct cggccaggcg ttcaacagga tgcgccgtag cctggaaaag
     8401 gcgatgagcc tgattgataa ggggtaagag gcaggatgaa cgccttaccc gcaggctacg
     8461 cgctgcacga ataccgcatc gacaaggtgt tgggcgtcgg cggcttcggg ctgacctatc
     8521 tcgcggtcga ctccaatctc aatttgaagg tggccctgaa ggagtacctg ccggcggaca
     8581 tcgccctgcg cggcgcgaac cagtcgatcg gtccgcgctc gcccgacacc gccgagacct
     8641 tcggctgggg caagcagcgc ttcctcgacg agtcgcgcac cgtcgccacg ttccgccacc
     8701 cgaacatcgt gcgcgtgatg cgcttcttcg aggcgaatgg caccgcttac atggtgatgg
     8761 agttcgtcga aggctcagcg ctcggcgact ggatcaagac gcggcggccg ctgagcgagg
     8821 cggacattct gccgatcgcc gtgcaggtgc tcgacgggct ggaggtggtc cacaaggccg
     8881 gcttcctcca ccgcgacatc aagcccggca acatttacat ccgcgaggac ggcaccgcag
     8941 tgctgctcga cttcggctcg gcgcggcaga aggcgagcga gctcacggcc gtcgtctcgc
     9001 caggctatgc gccgttcgag cagtaccact cgcatggcaa gcaggggccg tggagcgata
     9061 tctacgcgtt cgcgggcgtg ctctactgga tggtgaccgg ggggatgccg caggaagcgc
     9121 cggcgcgcat tcgcgccgac aagatggtgt ccgccgtgga aggcggggac cgcactctct
     9181 ttcgtaccga gttcctggcg gcgatcgact gggcactcac tccgttcgaa gatcagcggc
     9241 cgcaatccat cggggaatgg cgcgaggccc tgctgatcgg agggccgcca ccggcgaaac
     9301 cggtcgagaa aaagccggtt gaaaccaaac tcgttgaagc aaagacggaa aaggtaaagc
     9361 cacccaaggc caccactccg ccaaggctcc cgcccgatat cctccttcgc gcgcaacagc
     9421 gcctggcgga atacatcggc ccggtggcga ccgtcgtcgt caagcgggcg gcggcaaagg
     9481 cgcgcgacga agccgagctc tatctcctgc tggccgatga aatcgaggac aaggacgaga
     9541 agaaggcttt cgtgcgccag gcggtttcga tttccggcaa gccggcgagc taaaaaaagc
     9601 ttgacgcccg aggcggggcg cgactagcat tcaaacgttc gtttgaaagt cactcgtttg
     9661 aaagctacgt ttgaaagcca gagtgcccat gtccatccgc ccgatcaagc cccagcacga
     9721 aacccgcacc cgcatcctcg acgccgccga agagcttttc atgctgcacg gcttcgaggg
     9781 cacctcgatg cgccacctca cggcgcgcgc cggggtcaat ctcgccgcgg tcaactacca
     9841 cttcggctcg aagcacgcgc tgatcgaggc ggtgttccgc cgccggctcg atccgatgaa
     9901 cgccgagcgc gtcgccgagc tggcgcggct cgaggcgagc aacaatctct cgcccgatag
     9961 catcatccgg gcgttcgtcg gcccggggct gcgcatgatc gaggacgcca agggcggtcg
    10021 caacttcatc cggctgctcg gccgcaccta caccgagccc gcgaagaccg tgcgcgcgct
    10081 gatcggccag atgtacgcgc ccgcgatgga gcgctacaag agcgcgctcg agcgcgcgct
    10141 gccgcagatg ccgggcgacg agctggtgtg gcgcatgcac ttcatgttcg gcaccctcgc
    10201 ctacacgctc gccgcgaccg acacggtgca gctcatcgcc ggctgcaagc ccgaggaccg
    10261 ctacgacgcg cgcctgctgg aggaccgcct caccgcattc ctcgccgcgg gcctgctcgc
    10321 gccgctcaag agctcgatca agaaggccgc ctaggagatc tcgccatggc ctggtttatc
    10381 gccggtgtct tcgccgtcat cctcgtcttc ggctacttcg ggctgccgat cctctggtgg
    10441 acgatcgccg ccgcgctgct cggctggtac ctcagcgtca tcgcgggttt tgggttcatg
    10501 accaacgtgg tcctgggaat cgctttggtc gcgatcgcgg ccgtgctgaa catcccggca
    10561 ttgagaagaa tgctgatcag cgaccgcgtg cttgccatct accgccgcat cctgccggac
    10621 atgtcgcaga ccgagaagga agcgatcgac gccggcacgg tgtggtggga cgcggatctg
    10681 ttctccggca agcctgactg ggacaagctg ctcgccgtgc ccgagccgcg cctttcggcc
    10741 gaagagcagg cgttcctcga cggcccggtc gaggagctgt gcgcgatgct gaacgactgg
    10801 gagatcacgc acgagcgcca ggacctgccc gagcgcgtgt ggcagttcat caaggacagg
    10861 ggcttcctcg ggatgatcat cccgaagcag tacggcggcc tcggcttcag cgcgctcgcg
    10921 cactcggcgg tggtgatgaa gctctcgacg cgctcgagca ccgcggcgat ctcggtgatg
    10981 gtgccgaact cgctcgggcc gggcgagctc ctcatgcact acggcaccga gcagcagaag
    11041 aaccactacc tgccgcggct cgcgaaggga ctcgaagtgc cgtgcttcgc gctcaccagc
    11101 cccgaagccg gctcggatgc cgccgcgatt cccgacttcg gcatcgtctg caagggcacc
    11161 tggcaggggc gcgaagtgct cggcatgcgc gtcacctggg acaagcgcta catcaccctc
    11221 gggccggtgg cgactttgct tggccttgcc ttcaagcttt acgaccccga caagcttctg
    11281 gggtcgcaaa ccgatctggg catcacctgc gcgctgatcc ccacgagcac ccccggcgtg
    11341 aacatcggcc ggcgccacct gccgctcaac caggcgttcc agaacgggcc gaactcgggc
    11401 aaggacgtgt tcatgcccct cgactggatc atcggcgggc ccgagtacgc gggcaaaggc
    11461 tggatgatgc tcatgggctg cctcgctgcc ggtcgcgcga tttctctgcc gacctcgtcg
    11521 gtcggcggcg ccaaggcgct cacgcgattc accggcgcgt acgcgcgcgt gcgcagccag
    11581 ttcaagacgc cgatcggcaa gctcgaaggc gtggaagaag cgctcgggcg catcgccgcg
    11641 cagtgctaca tgatggacgc gacgcgcgtc atgaccgcgg gcgcggtcga tctgggggaa
    11701 aagccggcgg tactttccgc aatcgccaag taccacatga ccgagcgcgc gcgggactgc
    11761 gtgaacgacg gcatggacat cctcggcggc aagggcatct gcctcgggcc gaacaactgg
    11821 atcggccgcg gctaccagct ctccccggtc gggatcacgg tggaaggcgc gaacatcctg
    11881 acgcgcacgc tgatcatctt cggccagggc gcgatccgtt gccatccgta cgtgctgcgc
    11941 gaaatgcgcg cggcgaagga aatgcagggc gccgaggcgg cgcgcgagtt cgacgacgcg
    12001 ttcacctcgc acatcggcca cgtgctgcgc aacgccgtgc gtacttttgt ttttggactc
    12061 accaactccg cattcgcccc ggtaccgcga aacgctgcgt cggaaacgaa gcactactac
    12121 cgccacgtgt cgcgcctgtc cgcagccttc gccttcctcg ccgacatctc gatgctggcg
    12181 atgggcggcg cgctcaagcg caaggaaaag atttccggac ggctcggcga tgtgctgtcc
    12241 atgctgtacc tcgtttccgc gacgctgaag cgctacgagg accaggggcg catccgcgag
    12301 gacctgccgc tcgtgcgctg ggcggtgcgc gatgcgctgt accgcgcgca gcgggcgatc
    12361 gacgggatcc tgtcgaactt cccggtcaag gcgctcgcca cgctcctgcg ctggagcatc
    12421 ttcccgctcg gcatgccgta ccggccgccg ctcgactcgc gcaaccacga gtgcgccaag
    12481 atcgcgctcg agcccggcgc cgcgcgcgac cgcctgaccg ccggcatgtt cgtgccgaga
    12541 aacgaatccg acgccaccgg ccggctcgaa gccgcattcc tcgccaccat cgcttgcgag
    12601 ccgatcgacg agaagctgcg caaggccgtg aagaagggca agctcgtcct gcaacccggc
    12661 aaggatctcg gttctctcgc cctcgagaaa gacctgatca cgggtgacga ataccagcag
    12721 tggagccgca aggaagcgct gcgcaagggc gtgatcaagg tcgacgattt cccgcaggac
    12781 ttcggccgcg ccgcgctcgc cgccgcgcag gaaggcgaca ccgtcaagat gaaggcggcc
    12841 tgagatggtc gccgaccgat cggtttacgt ggtggacggc gcgcgcacgc cgttcctcaa
    12901 gtcgaagaac cgccccgggc cgttcgcggc cgccgacctc gccacccagg cgggccgctc
    12961 gctgctcgcg cgccagcgct tcgctccgga tgaactggat gaagtcatcc tgggctgcgc
    13021 cgcgccgtcg gtcgacgagg tcaacatcgg ccgcgtcgcc gcgttgcgca tgggctgcgg
    13081 ccagaaggtt ccgggctgga ccgtgatgcg caactgcgcc tcgggcatgc aggcgctcga
    13141 ctcgggcatc agcaacatcc tcgccggccg ctcgagcctc gtgcttgccg gcggcgtcga
    13201 tgcgctgtcg cgcgcgccgc tcctctacgg cgacaagatg gtgctgtggt tttcagacat
    13261 ggcctccgcg cgcaccacgg ggcagaaaat ctctctcttt ggaagacttc ccgtaagagc
    13321 gctgctggcc ccggtcatcg gcatcatgaa agggctcacc gacccgatgg tcgggctgct
    13381 gatgggccag accgccgaga acgtcgcgca ccgcttcggc atcacgcgcg aagagatgga
    13441 catgttctct gtcaggagcc acgagcgggt gattcgtgcg caagaccaag gcacgctcgc
    13501 ccccggcggg ggcgaggtcg aggcgctcta cgacgccaag ggcaacgtct acacgctcga
    13561 cgacggcgtg cgacgcgacg cctcgatcca gaacctggcg aagctgaggc ccttcttcga
    13621 ccgcaagtac ggcaacgtca cccccggcaa cagctcgcag attacggatg gcgctgcctg
    13681 ggtgatcctg acctccgaag aagctttgaa gaaacacgac ttgaaaccca ttggtcgcat
    13741 cgtcgactcc gaatgggccg gcctcgaccc ggcgacgatg ggcctcggcc cggtgcacgc
    13801 ggcgacgccg atcctcaagc gccacaacct cgggttgaac gacgtcgact attgggaaat
    13861 caacgaggct ttcgcggcgc aagtgctcgg ctgtctcgcg gcctgggcgg acgagaagta
    13921 ctgccgggaa gagctgggcc tcgacaagcc gctgggcgag ctcgatgagg aaaagctcaa
    13981 cgtggacggc ggcgcgatcg cgctgggcca cccggtgggc gcttcgggcg ccaggatcgt
    14041 cctgcatctc ctcaagactc tgaaacgcaa caacgcgaaa cgcggcatgg cctcgatctg
    14101 catcggtggc ggcctcggcg gagcgatgct ggtggaggca ctctgatgga acgagccgat
    14161 gccaaggtcc tcgcgaagca ctggcgctgg gagaaggacc gcgagggtct cgcctggctc
    14221 accttcgaca agcagggcga gtcgaccaac accttctcgc gcgaggcgct gaacgagctc
    14281 tctctgatgc tcgacgagat cgccgcggca aggcccaagg gcctggtcat ccgctcggcc
    14341 aaggacagct tcatcggcgg cgccgacatc caggagttcg ccaacttcaa gggccccgac
    14401 gacgcggtcg ccttcacgcg gctcggatgg gacactttcc agaagctgcg cgacctgccc
    14461 ttccccacga cggccatggt gaatggtttc tgcatgggcg gcggcatgga aatgtgcctc
    14521 gcctgcacct acatcgtggc gctcgacgac ccgaagacgc gcttcgcgca gcccgaggtg
    14581 atggtcggcg tgatccccgc gtggcacgcg atggaatggc tgccgcagcg cgtgggtccg
    14641 gccgcagcga tggatctgct cctcaccggc aagaccatcg atgccaagcg ggcaaagcgc
    14701 atcggccttg ccgacgaggc ggtgccgctg cgcatcttcg agaacaccgc gcgcatgatc
    14761 acgctggaag cgccgcccag gaagaaactc tctttcgtga accgcctgat gctcggcccg
    14821 ctcaagggga tcgttgcctc gcaggcgcgc aagcaggtcg cacgcaaggc gaagcgcgag
    14881 cattaccccg tgccgtacgc gatcatcgac gtgtggaaga acttcggcgg caacgcgctc
    14941 atcgtgccgc cgaaccatcc gagctccatc ccgacgctca tccagcaccc gaccggccgc
    15001 aacctggtgc gaatcttctt cctgcaggac cggctgaagg cgctgggcaa gggcgtggaa
    15061 ttcaaggcgc gccacgtgca cgtcgtcggg gccggcgtga tgggcggcga catcgcggcg
    15121 atctgcgcga tgcgcggctt gcgcgtcacg ctgcaggaca cggcgcccga gcgcatcgcg
    15181 cccgcggtga agcgcgccgc cgagctgttc aagcggcgcc tgcgcgacaa ggcccgggag
    15241 cgcgacgcgc tcgaccggct gattcccgac gtgagcggcg aaggcgcgcg ccaggcggac
    15301 gtgctgatcg aggcgatctt cgagaacctc gaggcgaagc gcgcgctctt cgcaaaactg
    15361 gaatcgctgg cgaaacccgg agccatcctt gcgacgaaca cgtccagcct gaagctggcc
    15421 gacatcgcga ccgcgctcaa ggatccgtca cgactcgtgg gcattcactt tttcaatccg
    15481 gtgcccttgc tgcaactggt ggaagttgtc agcggggaca ggacaaaccc tgaacttgcg
    15541 aaaaaggccg cggcattcgt gcgccaaatt gacaagctgc cgttgccggc gaaggactcg
    15601 cccggattcc tggtgaaccg ggtgctcggc ccgtacatgc tgaacgcctt ccgcatgctc
    15661 gacgcgggcg ccaagcccga gacgctcgac cgcgcgatgg aggacttcgg catgccgatg
    15721 ggtccggcgg agctcgccga caccgtgggc ctcgatgttt gcctggcggc aggcaaggcg
    15781 ctcgcgaagg gcgcgacttc ggtgccggag atcctcgcga acaaggtggc gctgggccac
    15841 cttggcaaga agacggggca aggcatctac cgctacgagg acggcaagcc ggtgaagggc
    15901 cagcccgacg cgtacgacca ggcgctcgtg gatgcgctga tcgagcctta cctgcgcgaa
    15961 gccgaggcgg tcgttgccga gggcatcgtc gccgacgccg acctggtcga tgcaggcctg
    16021 atcttcggca ccggctttgc accgttccgc ggcgggccgc ttcactatat aaagacgcgt
    16081 ggaagacaag ctccctgaag ggaaggtccc ggcgctgcgg gtcatgccga tgcccgcgga
    16141 cgcgaatcag aacggcgaca tcttcggcgg ctggatcatg gcgcaggtcg acgtcgccgg
    16201 cggcaccgcc gccgggcgca tcgcgcgcgg ccgcgtcgcg accgtcgccg tcaaggagtt
    16261 cgtgttcaag cagccggtgc agatcggcga cctgctctcg ttctacgtgg atctcgtgcg
    16321 cgtcggccgc acctcgatca cggtgaacgt cgaggtgtac gccgagcgcc ggccggccga
    16381 tccgaaagtc gtcaaggtga ccgaggcgac cctgacctac gtcgcgatcg accgcgacgg
    16441 gcgtccgcgc ccggtgccga ccgcctagat tgctgctcga cgcgccgacg ctcgccgcgg
    16501 tcgcggctct cgcggtcgtc gcggcgatca tgagcggcat gagcggcttc ggcgccggca
    16561 tcatcctcac cgccgcgctg gtgccgctga tcggggtgaa ggccgtgatc ccggtgctgg
    16621 ccgtcgccgg cgtcgtcatc aatgccggcc gcttctggtt ctaccgcact cacctcgacc
    16681 ggcacatgat cgcgctggtg ctcatctcct cgcttccgtt tctggtcctg ggcacctggc
    16741 tctacagtgt gctggacgcg cgcgcgctca gcgtcgtgct cgggctcgcg gtcatggcct
    16801 cagtgccggt gcgcagggtc ttgcatcaca agaagatcac ctttggatcc acgggcgtgg
    16861 cggtgggctc gggcgtgttc ggcttcgccg ccggcatcgc cagcggcgcg ggcgtgatcc
    16921 tggtgtcgct cctgctcggc gccggcctcg ccacgccggc agtgctcgcc accgacgcca
    16981 tgatctcgat cgtgatcgac attgccaagg cagcgctgtt ccagcgcttc gacctgctcg
    17041 acaccgcggc cttcttcacg ggcgtagtga tcggcgtggc gagcatcccg ggcagcgcga
    17101 tcgcggcgtg gctcgtgtcg cgcatgggtg cacggctgca cgtcctgttc atggaagtcc
    17161 tgatcctgtt cggcggcgcg tcgatgctgt ggcacgcctg gagatagcac gatgagaaac
    17221 ctgaaaggca gagttgccgc cgtcaccggc gcggcgagcg gcctcggccg ctcgatggcg
    17281 ctcgcgttcg ctggcgaggg catggatgtc gcgctcggag acgtcgatga gaagaacctc
    17341 tcgcgggtcg agaaggaagt gagcgcaaga ggcgtgcgca gcatcgcgct gcgcatcgac
    17401 gtgtccaaag cacaggacgt cgaagctttc cgcgatctcg cgctcgaccg cctggggggc
    17461 gtccacctcg tgtgcaacaa cgcgggcgtg tcgccgctcg gcgcggtgtg ggagagcacg
    17521 cccgccgact ggcaatggac cctcggcgtc aatctctggg gcgtgatcca cggcgtgcgc
    17581 gccttcacgc cccacctgat cgcgcagaac gaagggcaca tcgtcaacac cgcctcggtg
    17641 tccgggctga tctcgccccc ggggtccggc gcctacaacg tcaccaagca cgcggtggtg
    17701 acgctctccg agacgctcca ccacgagctg cgcgagcgca atgccgcggt cggcgtatcg
    17761 gtgctttgtc cggcctatgt gccgacggga atcgtggatt ccgagcgcaa ccggccggag
    17821 gatcttcccg cttcggaaag atcgccggaa acgctcgcgc gccaggcgat gctgcgcaaa
    17881 gcggtggcct cgggaaagat ctccgccgac caggtcgcgc aggccgtggt ggcggccgtg
    17941 aaggaggagc gcttctacat cctcacgcac ccgcgcatca agggcgcgat ccaggcgcgc
    18001 atggaagaca tcctcgaggg ccgcgcgccg cgcaatccga tgaccctcta gcgatggcgc
    18061 tttgacgcgc acgctcgtcc tgctgcacgg cgcggcgagc aactcgacgc gctgggcgga
    18121 gttcatcacc catacgaact tgcggggcga gtggaagctc gttgctccgg acctgcgcgg
    18181 caacggcacg gcggccgcgc cgaggacgcg tctcggcatg gacgtctggt gcggcgatat
    18241 cgtgaagctg ctcgacgccg agcgctgcga gcgcgcggtg atcggcgggc actgcttcgg
    18301 cgccaacatc gcgctgcact tcgcggcgcg ctatcccgga cgcatctcgg cgctcgtgct
    18361 gatcgagccg atgccgcggg aggcgttcgt cggctctgcg gcctggctgc ccttcttgcg
    18421 gccgctcctc tacctgacct acggcgccgc ccgcgcgctc aacgcgtctg ggatttaccg
    18481 gcgcaggctc gaaacgctcg acctggaaga atgggaccgc ccgaggcgca gcggcgagcg
    18541 caaactctcg atgctcgctt cgctcggcgt cgacctcgga gccacgccgc tggcggctta
    18601 cacgcgcatg ctcgcggcga cggccgagcc atggcccgat gtcgcggcga tccgcgtgcc
    18661 ggtcctggcg ctcatttcct cgcgcagcag catgaccgac cctgcgcgca cgcgccgcgc
    18721 gctcgccatg ctgccggatt gcgatctcct cgagctcgac gccgtgcact ggattcccac
    18781 cgagcagccc gatgccatgc gcgcggcaat cggggactgg atcagccgac ggctcggttc
    18841 ttcgccatga gggcggcttg cgccgccttg cggtcctcgt tcaccctgct gaaggccggg
    18901 cgctcgccga gcatcttgag atagggcttg acctgcggaa tgtcctcgag cacgtcgcgc
    18961 ccgaggccgt gcttcgtcgc cagggacacg agcggcaggt ggacgaacgc ggcgcaatcg
    19021 gcgagcgtga gctccttgcc cgcgatgtac gggtcgaact tcaccagcgc cttgaaggcg
    19081 cggattcctt tcgcgaggtc cttctccgcc gcctgcttga cctcgtcgga gaccgggcgc
    19141 ttccagaaca cctgtccgta cagccggcgc gccaccagct ccacatgcag ctccatgaac
    19201 ttgatgatct cgcggacttt cgcgcgctcg aacggatcct tcggcaggag cggcttctgc
    19261 ggatacgcct cctccaggta ctcggcgatc acctcggatt cggccaggcg ccggccattg
    19321 tcgagctcga ggaacggcac cttgccgatc ggcgagcgcg ccaggaactc gtccttctga
    19381 gaagggctgc agccgggatc ctcctcgaac gccacgccct tctcgagcag cgcgatgcgc
    19441 agcttgttgt ggtagttgct gaggtggaaa ccgcagagtt ttagcatggc tggattatag
    19501 tcagcgcatg ccggacccga agcccgaatt cattaccgat ctcgccgccg acacctctta
    19561 cggcggctac ctcgggctcg agcggctgct ctcggcgcag cagccgcgct cgtcgcacca
    19621 cgacgagatg ctgttcatca tccagcacca gacttccgag ctctggatga agctcatgat
    19681 ccacgagctc gccgccgcga tcgggcatgt gaaggcggac cggctcgatc cctgcttcaa
    19741 gatcctggcg cgcgtgaagc agatccagcg ccagctcttc gagcagtggg gcgtgctcga
    19801 gacgctgacg ccgtccgagt acgccgagtt caggaacgtg ctcgggcccg cctccgggct
    19861 gcagtcgcac cagttccgcg ccatcgagtt cctgctcgga aacaagatcg cgaatcgcgt
    19921 cgacgtgcac cggcacgatc cgcagaccac cgcctacctc gaaggcctgc tgcgctcgcc
    19981 cagcctttac gacgagttcc tgcgctacct ggcgaggaag ggcttgccgg taccgcccgg
    20041 gcgcgtcgag cgcgactggt cgcagcccta cgagaaacat ccaggcgtca ccgcggtttt
    20101 caagatcgtc tatcaaaata caagctccta ttgggccgag tacgacatgt gcgagaagct
    20161 ggtggacgtc gaggagaact tcctcctgtg gcgcttccgg cacgtcaaga cggtcgagcg
    20221 catcatcggc ttcaagcgcg gcaccggcgg cacggccggc gtcccgttcc tgagaaggac
    20281 gctcgagacg agcctgttcc ccgagctgat cgacgtcagg accgagcttg gatgagcgct
    20341 ggctaaggaa ggagatcttc ccgaagttca gccgggtgct gcaacgtaac gagatctatc
    20401 tggcgaatca ctcgctcggc cggccgcccg accgcgtcgc cgacgacgtg cgcgccgcgg
    20461 tcgatgcctg gtaccgcgac ctggacggcg cgtgggattc ctggctgcag caccgggagc
    20521 gcttcaggtc gctgacggca aagctggccg gttcgccgcg cgccgactgc atcatcccga
    20581 aaaccagcgc cggccagggg ctgcgcgcgg tcctgaattc gttcgcgacc aagcccagag
    20641 tggtgacgag cgacggcgaa ttcgactcga tcgacttcat cctgcgggtg taccgcgagc
    20701 aggcacgcat cgagctcaag atcacgccgt ggcaggagct ctccgcgcag ggcgcggatc
    20761 tcgtggtcct gtcgagcgtc atgttccgca ccggcgagat cgtcaagcat ctgccgaagc
    20821 tggtccacga cgcccacgcg gcgggcgcgc tggtgctgct cgacgtctac caccacgccg
    20881 gcgtgctgcc gctcgacctc gccgcgctcg acgccgactt cgccgtcggc ggctcctaca
    20941 agtacacgcg cggcggaccc ggcgcgtgct ggctgtacgt gcgtcccggg ctggtcgaga
    21001 cgctgcgcac gccggatacc ggctggttcg cgaagaaaga cgtcttcgcc tacgcgcggc
    21061 cggagccgcc ggagttcgga cccggcggcg acgcctggct cgagtcgacc ccgccggtgc
    21121 tggcgtgcgt gcaggccctt gccggcctcg agctcaccct ggcgctcggg gtggagcggc
    21181 tgcggaaata ctcgctcgaa cagaagaaga ttctttccga cctgatcccg ggctgtgaag
    21241 gcgccgcgga gagccacggc gctttcctga ccttcaggca cccgaacgcg gctcttctct
    21301 gcctgaagct cgaacaacag ggcgtgagag tcgatgcgcg tggcgagtac ctgcgcctcg
    21361 gcccggacat cctgaataca caggcggagc tggagaaagc cgccgcggtc ctgaaatctc
    21421 tgctttccta gaagcgctcg gcgcgatacg gctgcggatc gatgaagggc gcctcgccag
    21481 tgatcatctc ggcgatcagc cggcccgtga ccgggccgag cgtaagcccg tggtgcgcgt
    21541 ggccgaatgc gaaccacagg tgcgggtggc gcggcgcggc gccgatcacc ggcatcatgt
    21601 ccggtgtgca cggccgcgag cccatccaag cctcgttgtc gagccgctcg gcgagcggga
    21661 acaggtcgcg cgcgatcggc tcggcgcggc cgagctgcac cggggtgcgg atcgcgtcgc
    21721 gccgggcgaa ctcggcgccg gtcgtaagcc ggatgccgcg gcgcatcggc gcgaggaagt
    21781 agccgcgctc ggtgtcgagg atcgggtggt tgagcacggc gcgtcccgcc gggcggtagt
    21841 gcatgtggta gccgcgcttc accgcgagcg gcagctcgta gccgagcgcc cgggtcacaa
    21901 cgtcggacca gggcccgagc gcgattaccg cgtcgcgcgc gccgaccggg ccgtcgacgg
    21961 tccgcacgcg ccagccgttg ttgttttcca gacttgcagc attgccctgc gcgaagcggc
    22021 cgccgagctt ttcaaagagt ctcagataac tgagcgcgag gccctgcgga tccaccaccg
    22081 ccaggggatc tgtccagtgg agcgcgccga ccagcaccgg ggcgagatgc ggctcgagcc
    22141 cctgcagcgt gcgcgcatcg tgcgccttgt agttgagccc gaactcgccc cgccagcgct
    22201 cggcttcggc gaagcgcagg tcgcgctcgc gcgcgctgcg gaacaccttc atccagccgg
    22261 tgcgccggaa gaggtcctgt gcgcccgcgt cggcggcgag cgcctcgtgc tcggtgatgc
    22321 aatgctcgat cagcttcgcg tacagccggg ctatagcctg gtgccgcgcc gggcgcgagt
    22381 acttccagta ctgccacagg aacggcgcga ggcgcgggat cgcggaaggg tggtaatgcg
    22441 cgtcgatcgt gcggttcagg ctgtagcgga agagcgcgcc gaagtcgtgc gggaagccgt
    22501 agggatagac gccttcgcgc tgcaccagcc ctgcgttgcc gaacgaggtt tcctcggccg
    22561 ggccgcgccg gtcgacgagc accgtcgcgc gcccgcgctt ctgcaggtga agcgcaaccg
    22621 agacgccgac gattcccgcc cccaggacga gggcatctgc gcttttcaag ttatcgagaa
    22681 accgtcttct tcaaggactc ggcaatgctg gtctgctcgc cgagctcgag cttggcgatc
    22741 tggttgcggt gcacttcgtc cgggccgtcg gcgaagcgca aggtgcgcgc gtgcgcgtag
    22801 gagtgcgcga ggccgaagtc gtccgacacg ccgccgccgc cgtgcacctg catcgcccag
    22861 tcgatcacct ggcaggcgac gttgggggcg aacaccttga tcatggcgat ctctttcttc
    22921 gccaccttgt tgcccaccgt gtccatcttc cacgccgcgt tcagcaccat gtagcgcgcg
    22981 gcgtcgatca tgatgcgcga ttcggcgatg cgctcgcgcg tcacgccctg atccgagacg
    23041 ggtcttccaa aagcaaccct ctctgtggcc cgcttgcaca ttttctccag cgcgcgctcg
    23101 gcctgcccga tcagccgcat gcagtggtgg atgcgtccag gcccgaggcg cccctgcgcg
    23161 atctcgaagc cgcgcccttc gccgaggagc acgttggaag cgggcacgcg cacgcccttc
    23221 agctcgatct ccatgtggcc gtgcggcgca tcgtcgtagc cgaacaccgc gaggtggcgc
    23281 aggatcttga cccccggcgt acccgcgggc accagcacca tcgattgctg gctgtggcgc
    23341 gaagcgttgt cggggtcggt cttgcccatc acgatgtaga tcttgcagcg cgggtcgccg
    23401 gcgccgctcg accaccactt ggtgccgttg attacatatt catttttttc acgagcaatt
    23461 cttgtctgaa tgttggtcgc atcggatgag gcgacgttcg gctcggtcat gaggaacgcc
    23521 gagcggattt ttccctcgag cagcggctcg caccactcct tcacgtgctc gggcgtgccg
    23581 tacttgagga gcgtctccat gttgccggtg tccggcgccg agcagttgaa cacctcgggc
    23641 gcccaggtga cgcgtcccat gatctcgcaa agcggcgcgt actcgaggtt ggtgagcgtg
    23701 ccgccgtgcg acttcggccg ccacaggttc cacaggccgg ccgtgcgcgc tttgagcttc
    23761 aattcttcaa tcaagggaac gggctgccag cgttgctcgc cgctgacgtg cgcgtgccac
    23821 tcgtgctcgc gcggatagac atgctcgtcc atgaaggcgg cgaccttctt gcgcaggtcc
    23881 tgcaccttgg gcgtgtaatc gaagttcaag cggccctcct cagtatcttc tccacttgct
    23941 gccatccgag atcggcgagc ggaactgtct tgcttgccgc ctcctgggcg aacgtgctcg
    24001 ccgcggtgcc ctcgagcacg cgcttggcga tgccctggca gatcgcggcg atgcgaaaca
    24061 ggttgtacgc gagatagaag tcccagtgcg acggatcgat ggactttcga tgcgttctcc
    24121 tgcaataggc ggccacgtat tcgctttcgc cggggattcc cagctccttc aagttcaacc
    24181 cacccatgcc gcggaacttg tcgggcggga tgtgccagct catgcagtga taggcgaaat
    24241 ccgccagcgg atcgccgagc gtcgagagct cccagtcgag caccgccagg atgcgcggct
    24301 cggtcgcgtg gaagatcgtg ttgtcgaggc ggaagtcgcc gtgcaccacc gaggttcccg
    24361 cctcgggcgg gatgttcttc ggcagccagc cgatcaggct gtccatcgct tcgatgcgct
    24421 gcgtctcgga ggcgcggtat tgcttgctcc agcgctcgac ctggcggccg atgtagtttc
    24481 cgggcttacc gaagtcctcc aaacccaccg cgcgatagtt caccaggtga agttgtgaaa
    24541 tcaccctgtt ctgctcgtcc cagatcgcgg cgcgctcgcc gcgcgtcatg ccgggaagcg
    24601 ccggatccca cagcactcgg ccctcgacgt aatccatgac gtagaacgcg gtgccgatca
    24661 ccgagtcgtc ctcgcacagc acgtgcggcc ttgcgacggg aaaccccgtg gggtgcagcg
    24721 ccttgatcac cttgtattca cgctcgacgg catgcgcgga gggcagcagc ttgccggggg
    24781 gcttgcgtcg caggacaaaa cgaccttctg gagaagaaat ccggtacgtg gggttcgact
    24841 ggccgccctt gaattgctcg atgctcgaga tcttgaaatt gaggaatttc tcgagcgcgg
    24901 cgaggtcgat ccggtggcgc tcctcgaccg gctttgtacc gatgaactgc tcgaacatgc
    24961 cggcaaaagc ttacattaga atcgtccggc gatgcgcttc agcgtttccc attcgaaggg
    25021 cgcgagattc gcgcgccgcg ggctgcgcag ctacttcgag taccgcgacc tcggcatcaa
    25081 gcgcgccacc cgcggcaagg tggtcgcgca cgtgatccgc gcgcgcgtgg gcaaggcgcc
    25141 gcacggggaa tggcacgcgc acgattgcaa ggtgcagttc gtgtatgtcc tcaagggctg
    25201 ggtgctgttc gagtacgagg gcgtcggccg ggtgatgatg aaggccggga gctgcttcta
    25261 ccagccgccg cgcatccgcc accgcgagat ccggcactcg aggaacctcg agatgatcga
    25321 ggtggtctcg cctgccgcct tcaggactta ttctgcgtct tcggcgaagc ctgcagccag
    25381 aaagtcaccg ggccgtcgtt cacgaggctg acctgcatgt gggcgccgaa ccggccggtg
    25441 gccacgcgcg gcgagcattc ctggatcttc tcgacgaatc gcctgaatag cttctcggcc
    25501 tcggcgggcg tcgccgcgct ggaaaagctt ggccgcaggc cgctgttggt gtccgcggcc
    25561 agggtgaact gcggcacggc gagaatctcg ccgcgcacgt ccttcaccga gaggttcatc
    25621 ctgccttcgg cgtcggcgaa gatgcggtag ttcgtcacgc gctcggcgag gctcagcgcc
    25681 tcggcctcgg tatcgccgcg ctcgacgccg atcagcgcga cgatgccggc gccgatcgct
    25741 cccaccggct cgccggcgac cttcacgtcc gccccggtga cgcgctgaat tagggcgatc
    25801 aatgcggcgc tatcaggcaa tgatgtcggg aatcaggcgc gcgcgcaggc gctgaatctg
    25861 gtctttcagc aggagcttgc gcttcttcag gcgttgcagt tggagctggt cgagcgagcg
    25921 gtcgatgacc aggcggccga tgacgtcgtc gaggtcatgg tgctcgacct cgagcgcgtg
    25981 caggcgctgg cggatttggg ctttttcctc ttcggtcaga ggagtatcgg tcacaacgtg
    26041 aaaaacatca tgccggtcgt gtgaatcgta tccctatact tggccgcgcg aaagccctct
    26101 atgcgtcgta gcgaccgcga cgtagccgac tcaatccaga ccaccgaccc cgccgccgta
    26161 ggcgacgagg tgatccgcct cgccaggcgg ctctttcccg acgcatccgc gcccaccctc
    26221 gagcgcgcct ttaccgacgc cgctcgcatg taccgcggcg agcaccgcga ctacctgccc
    26281 tgcgacaccg agtaccacga catccagcat atcctcgacg tcacgctcgc catggcgcgg
    26341 ctcatggacg gctaccagcg cggcaaacgc aacggcagcc ccgagctcaa caaggagctg
    26401 ttcctcgccg gcgcgctcgc agcgttgttc cacgatttcg ggtatctgcg ccggcgcaac
    26461 gatcacacgc accgctacgg cgccgagtac acgctgaccc acgtctcgcg cggcgctgcc
    26521 ttcctgcgcg ggtatgcgcg cgacctgggc gtcggcgagc gcctcgccca ggcggcctcg
    26581 accctggtgc acttcaccgg ttacgagcgc ccgatcgaaa gcatccggct gaacgacgtg
    26641 gcgctgcgca acctcggcct gatgctcggc accgcggaca tcatcgcgca gatgtcggac
    26701 cgctgttacc tcgagaaatg ccgcgaccgg ctgtatcccg agttcgtatt ggggcgcatc
    26761 cccaacttct cctcgggtcg cgacctggtg atgaaaacgc cgctcttcta cgagggcgcg
    26821 tgcaagcggc tcgacgacaa gctcggccgg agctacgact acgccgcgtg ccacttcggc
    26881 ggcccgaatc tctacctcga tgcgatgcgc aagaacgccg aatacgcgct atcgatctcc
    26941 gaatcgggcc agttcacgct gcggcgcaac ccaccgaaaa cgctgccgcc agacgtcgaa
    27001 ccctatccga aggacttaat aggttcttga aggcctgaag tactcgatgc ggtcgatcga
    27061 gcgcacggtg aggtccttga cgctcgcccc ctggagcacc ttgagcgtga tgttgctccc
    27121 ggcggcgccg cggccccaga ccttgcggta gaactcggcg agcgtcgtca cctcgtcggc
    27181 gccgaccgcg agcacgatat cgccgctctt cagcccggcg cgcgccgccg gcccgtcggg
    27241 cgaaacgcgc ccgacgaaca gacgtccccg gtactcgtcg gtgttgaggc cgagccacgg
    27301 gcgcgccggg ccgttgcgcc ggcccttcgc gatcaagtcc tcgagaatcg gcttgagcaa
    27361 gtgcaccgga acgaacatgt tgcccggcga ctgcgtgccg ggaccgccgg cgtccggaac
    27421 gatcagcgag ccgatcccga ggagctcgcc cttggcgccg atcagcggcg cgcccgacca
    27481 gtccatcacc gccgggtacg tatagatcgc ggaatcgagc aggtattccc aattgcccga
    27541 gaacgctcgg cgcgagacga cgtacaccag ggtgggactg tctcgcgccg ccgcgctcgc
    27601 gaccattgcc ggctcgcgct cgccgagcgc gaccgagtcg ccgagcggaa ggggtttcgc
    27661 tcccaggttt cccacgattt tcaacagggc aaacccgctc gcgtgatcgt agcctgcaac
    27721 cgtcgctggc atggtgcgcc catcggcgcc cgtcacctgg atggcttcgg cctcgatcac
    27781 gaggtagccg atggtcagga cgtagtcctt gcgcaccagc gcgccggtgc cgttccgctg
    27841 caggcccaga gtctcggcgc tgcgagcgtt ggcctggatt tttgcgctta ccccgaccac
    27901 tgcgccgagc gtgtcctcgg gcttgtcctg cgccgccgcc ggcaaagcgg cgaggcacac
    27961 agagagagca agggcgagcg ttcgcattgg cttctcctcg acacgcaata ataggccggt
    28021 tgagcgcctt ctggctaaac gagctcgcgc ggaccgcggt gcttttcctg accgcgtatg
    28081 ggctcggcgt ctgggtcaag cgcaccggca tccgggtcaa ctacacgcgc aagatcatgc
    28141 acttcgtcat cctgctgttt ccgatggcgc tcgccgcggt ttttccctat gaagcgagcc
    28201 tcttcaccgt cgtcgcgagc ggactggttt tcatcgcgag cctcggcctg ttcacccggc
    28261 cgctgcgcga gcgctcgagc atcgtcgcga ccgcgttcgg gtcgttcgat cgcccggaag
    28321 accggccgtt cacgctgctg tggctaagca cgcagctcct cgccacctat gcggtcctgg
    28381 tggtcatgct cctgtggctg gggcgctacg agaaagcgac gctcatctac atcaccgtgc
    28441 tggtggccgg catcggcgac ggccttgcgg agccggtcgg cgtgcgcttc ggccgccata
    28501 cctacagcac gcgggcgctc ttcaccgacc ggcggtacac gcgcagcctc gaaggaagcg
    28561 cgtgcgtgct cgcgagcggc tttctggcgg tgctactcct gcgtccccag ctcgacgaaa
    28621 tgcagttcgt cctggcgctc gcgatcatcc cgcttgccat gacgctcgcg gaagcccggt
    28681 cgccgcacac ttgggacggg ccgtttctct atctcgcggg cggcgcctcc accgtcgcga
    28741 ccttggagtt gtcgcgcctg ttgcaatagg gtacgccggg actactattc gtttatctca
    28801 tgttcctgac cgcgctcgcc gcttcgcttg ctctgggcgc aggcttgctt gccgcggcga
    28861 gctacagcga aggaacactc attgccctga ggaccgccgc gcaggctatt ggatggctgt
    28921 tcatcctggc gacggtggtc ggcctcacag gcttgcggca agccagtttc ggcaggctcg
    28981 gcgcggctac cgtcgcattc ctctccggcg taacgatgct gtacgtggcg tatttccagt
    29041 gggaccaggc tgtggaagct cccatccgcc acgccgctgc agaaacgctc tcggcgcgcg
    29101 agccggctta cagggtgacc ttgacgagcc tggaagccgt tacgcccgtt gcgatgaagg
    29161 aagtgccgcc cgagaacaag ccggtcatga aaaacgccgg cgatgcgtgc gcctctctaa
    29221 aaggcgtgga gtcgctgcaa tgcagccgtt gcacggcgaa gttcggggtg gcgtggatcg
    29281 tgtgccagga atcggcacgg ctggagtact gccggggccg ggaagacgac gacgcggctt
    29341 gcccttcggc tattccgttt tcgccgcccg gctgaagccg ctcatgaagc gggatagcag
    29401 attgcgcggc tttgccgcca cgcggggcgt caccggcgcg gcgggccgaa ccaccggaac
    29461 gggacgcggc tgcggccggg gttgtgctac tgtgaccacg ccgattgccg tcaatccggc
    29521 atccgtaatc cggaatatct gcgcgctcaa gtcttgaatc aggtctttcg caagcagccc
    29581 ggcatacgct ctctcgtacg cctggcagtg gatcggcggc cattgcgtcc tcagcgcctt
    29641 cagctgctga tcgcgcgctg ccgcgtagga atagagaacg cggatttggg ctagcaccgc
    29701 tctttcgccg ggagtgagat ccatgcgcta ttcctttatg tgcaccttga ccctagcctg
    29761 ccacaggcgg ggtcgcgccc ggactcccgc tgaagatctt ttgcaggatg aaaagcaaga
    29821 aaaggtcatc gcgccacatg cgcctgctta gctggaacgt gcagtggtgt cgcggcctcg
    29881 acggccgcgt cgatcccaag cgcatcgccg acgaggcgaa gcggatcgcc gaccccgacg
    29941 tgttctgcct gcaggaaatc gcccgctatt tccccgagat ggaaggcagc gcgggcgagg
    30001 atcaggtcga ggtcctggcg cgcgaattcc cggggtacga agcctttttc gcgcccggcg
    30061 tggacttgcc cggaaagcgc gggcgccggc gcttcggcaa cctggtcctc acccggcttg
    30121 ccgtcggccg ggtgctgcga caccagcttc cctggccctc ggcgtccgct gtgcgcagca
    30181 tgccgcgcgc cgcggtcgaa gtcgtggtcg aggctccgtt cgggctgctg cgagtgatga
    30241 cgacgcacct cgagtactac tcgaaggacc accgcacggc gcagatcgag cgcttgcgcg
    30301 agcttcacgc ggaggcgtgc gcggagcacc tccccgtgga cgagcccggc agctacgagt
    30361 cccacacgcg cgcgaagagc gcgctcgtct gcggcgactt caatctgaag cccgacgacc
    30421 cgggtcatcg ccagatgctc gacgcgggtt tcgtcgacgc atggctggcg ctgaaccccg
    30481 gcaagccgcg ccaggccacg ttccacctgc acgacggcga gccgccctat tgctgcgact
    30541 acatgttcgt cacccccgat ctcgtgccgc ggctgcgtgc gctacgcatc gaaaccgaga
    30601 cgcaggcttc ggatcaccag ccggtcattc tcgatcttgc ttgaggtcag aaaactcgcg
    30661 gatgcactgg gtgccgaaat cggcagcctc gatttgtcgc tgccattgac cgagcaaacc
    30721 tttagagaag tacgtgaagc gtggctcgag cacctggtga tccgcatccg cggccagaag
    30781 ctgtccgacc cggagctcct cgcgttcagc cgcaacttcg gcgagctcga cccgccgggc
    30841 cagaacccgc tcggacggcc gttcctcgcc gaccacccgg aaatgaacgt gatctcgaac
    30901 atcaagcacg acggcgtccc gatcggcggg ctgggcgacg gagaggcgat ctggcacgcg
    30961 gacatgacct acgtcgagcg cccgccgatg gcggctatcc tgtacgcgat cgaaatcccg
    31021 ccggcgggcg gcgacacgta ctgggccaac atggttgccg cctatgaaac gctgcccgag
    31081 gagctgaagc ggcgcatcga ggacctcgag gcaatccacg acgccaccta taacagcgcc
    31141 ggcacgatgc ggaagggcta cggcgaggtc accgatccgc gtaccgcgcc cggcgcgcgc
    31201 catccgctgg tgcgtacgca tccggaaacc ggccgccgga gcctgtttct cggccggcgg
    31261 cgcaacagct acatcgttgg tctggattta agtgaaagtg aaaacctgct cgatacgctc
    31321 tgggcgcacg ccacgcagcc gcggttcacc ttccggcagg aatggcgcgt cggcgacgtg
    31381 ctggtctggg acaaccgctg caccctgcac cggcgcgacg ccttcgaccc gcgcgcgcgg
    31441 cgcctcctcc accgaacgca ggtcagggcc gcctaggctc gagcagcctg cggatcttct
    31501 cgattctcgc gcggttgacg ccgaagtcgc gccggccgag gcgcgaggcc gagcgcacct
    31561 gcaccaccgc gccgtcgaag aagaactcca cgtcgtcgac gaaaccgaga agcttgctgc
    31621 ggaattccgc gtagaggtag ttcgagtgcg cgagcacgat gcgcgtgcgg ggcagcgctt
    31681 ccacggcttt cctcacctct tccatgctca ctctgagggg cgctatgtgg tgctcgcggt
    31741 cggacggatc ggcctgcgag gaaacgcaat tcggcgagcg cttgcagggc gcgaggcgcc
    31801 cgttcttcac gccgagatcg gcgggacgac gaaaggaaaa cgagacatcg aacatcgcgc
    31861 cggatgctac catcgcccga ctcttcgacg gaggcgacga tgacgccaca acaactcgcc
    31921 gagcttcttc tcggcatcgc ccgcgcgcag gccgcgatca tccaggggct ggagaacgag
    31981 atggccggcg tgcgcagcgg ccgcatcatc ccggcgctgc agaacgtcgc ccacctgcgc
    32041 gatcacccgc agccgacgct gaccgacctc ccggtgcgag tgctcctcgg aacgttgggg
    32101 cgccaggtgc ccgacgccgc cggcctcgtg cgcgacctgg aacgcctcct cagcaaaccc
    32161 gcagggtccg cctagcggga tcgatctcca ccgtcgcgcc gtgcggcacc cgcgccgtga
    32221 tgtcgccatc gaagccggcg agcagggtga cgccgccgaa cgcggcgccc tgcgcaagga
    32281 tcgggttcac gctgttgagg acgatcgcga gcggcaccac gccgcgcgaa gccatctcgt
    32341 gcagcatcca ggcggtcgcg acgccgcctt tcgcggtgtc gagcaccagc accttcccga
    32401 cgtaggactg gccggcgagc ttgtgcgcgg ggcgcgagaa cacgccgcgg atgcggtcga
    32461 ggtcgtagcg cgccgagaag ccgtcgtgcg cgaccagggc ctgcccgcgc gccgggccgc
    32521 cgatcgcgtg ccgcgcggtg aagatcatcg cggcagcctg ccgctttcgg ccgcctgcac
    32581 gcagcgctcc atcgaggcga gcagcggcac gtagccgtag ccgccgagga tgttgacgag
    32641 cttcacgctg ttggtcgcga gccgcttcca gccattcgcc tcggcgatct cgcgcgcata
    32701 cgactggtag aagcacatgc cggaaagtac gtgacctcct gcgtcttcaa tccttttcgt
    32761 caaacccagc cgatctgcat cgggcttgac ctgggggctg gtcaccgcga gcagcggccg
    32821 cgcaaagcgg cgcccgtcgc agagcgcagc gaggctgcgc agctcgtaaa gcgagagctg
    32881 cggcgcggag aacaccacga cgtcgatctc gtcctcgccg gcgtagaccg cctgaagcgc
    32941 tttgacgtct ccctccccga tccggtgcga aaccttcaac ggagaatcag ccgcctcggg
    33001 cgtgatgccg gcgagatgga acagcgcaat cgatccgaag ctcgccatcg ccgcgccgaa
    33061 gtgcttgagc tcatcggacc cgggcacgcg ctcgaggccc tcgacgtacg gcactgccca
    33121 gtagttgccg gccagacgcc cgaccaggcc gccgagcgcg ccccagtcgt tcaaggtctg
    33181 gggttgaaaa gaaacctgga ccctgagggt agcgcggcgg cactcatcca gatgaaaccc
    33241 gtagcgcggc gtgcgcccgg tgaggccggc cgcgaccgcg gaaggcccgc cctcgtagtt
    33301 ggagcgcgcc ccgcacaccg agttggcgta gatcaccacc ccggtgtcgc cgaacgcgac
    33361 gcgctcgccg cgcgccgggg cctggatggt ctggtagttg atgcaggtat cggtcatcag
    33421 gacgccgagc ttcttgaacg cgtccaccgt gcgccgctcc aggtccacga tccacgccgg
    33481 gtgcttgagc acgcctgcct tcgcgaagtc ggtgccgcgc gggtcggtga tggtgggcac
    33541 gcgcacgcgc cctccctgtg cgccgagaga ttccacccaa cgcacgccgg cctcgcccag
    33601 gctctcggtg tcggccatga tgtgcgcttg cgtcaccggc acgaagtcgg ccgcgccgaa
    33661 gaactcgccg accttgacct ggtgctcgat cgcggccttc gccgcggcgc cgagctcgcc
    33721 cgccagcatc gcgcgctcga ggtcgttcag cctcacatta gaagtccttc ggttcggcag
    33781 gaaatttcgg ctcgcgcttc tcgcgcagcg aggcgaggcc ttccttcacc tcggggccgg
    33841 tgaagcccag gatctcgaac gcgagcgagg cgtcgaagga cgggccggcc agccgcagcc
    33901 aattgttcaa actgtacttc gtccagcgca gcgcggtctg cgggccggcg gcgagcttgc
    33961 gcgcgacttc gagcgccttt tcccgcagct gcgactcttc gcaggtcagg gaaaccagtc
    34021 ccaggcgctc ggcttcctcg ccgctgatcg tgtcgcaggt cagcaggtag tacttcgcct
    34081 tcgccatgcc gcacaggagc ggccagatga tcgccgcatg gtcgcccgcg gcgacgccga
    34141 ggcgcgtatg gccatcgatg agccttgcat tcctggccgc aatggagatg tcggccatca
    34201 ggcccgccgc cagccctgcg ccgaccgcag gcccgtgcat cgccgagacg atgagcttcg
    34261 agcagttgag catgttgtag accaggtcgc gcgcctcgcg ccagacgcgg gcgcgcgccg
    34321 cgaagtcgcg cgtcatctcg tccaccatcg ccatgtcgcc gccggccgag aagcccttgc
    34381 ccgcgccgcg caggatcgcg acgcgcgtgt cggggtcgcg gtcgacgtcg agccagacgc
    34441 gcgccatttc ggcgtgcgca cgcacggtcg ccaccgcgag cttcccttcc tcgcccatca
    34501 cgatctcgag gatcccgggc tcgattcgat ggattcgcag atctttatac gcggagtagt
    34561 ccatgagtca ttctacaatt cgcgcgtgcg caccccggat gggagcttcg tcgatcttcc
    34621 cggttttccc tacgctgcgc attacctcga atggcgcggc ctgcgcacgc actacgtgga
    34681 cgagggaaaa ggcgatcggg tctatctgtg cctgcacggg gagccgacct ggtgctatct
    34741 gtaccgaaga atgattcccg cgctggtcgc ttccggaggg cgcgtcgtcg cgccggattt
    34801 cgtcggcttc ggccgctcgg acaagcccgg ggacgaagcg ggttacacct tcgactttca
    34861 ccggggcttc ctgctggaat tcatcgagca gaagcagctg cgaaacatca ccctggtcgt
    34921 gcaggactgg ggcgggctgc tcggcctgac cctgcccatg gagatgccgg agcgcttcga
    34981 gcgcctgctc gtcatgaaca ccacgctcgg caccggcgac gtcgcgctgc cggaaggctt
    35041 cgtcgcctgg cgcgcctacg taaggaagaa tcccgacctg ggctgcggca agctgctcgc
    35101 gcgcacctgt ccgcacctga ccgcggcgga ggccgcagcg tacgacgcgc ccttccctga
    35161 cgcgcgcagc aaggccggcg tgcgccggtt ccccgagctc gtgcccgagc gctacgacga
    35221 tcccggcgcg gcgatctcgc gcaaggcgcg cgactggctg agcacgtcgt ggaagggcga
    35281 gagcttcatg gcgatcggaa tgaaggatcc ggtcctcggc gaaccggtga tgcgcgcgct
    35341 gcagcgctcg attcgcaact gccgcgagcc ttatctcgtc gcgcagggag gccactttct
    35401 gcaggaatgg ggagaagagg ttacgcaatg gtttctttcg aagacatagc ggccgcgcac
    35461 gagcgcatca agccgcaggc gaagcgcacg cccgtgctca cctcggccac ggtcgacgcg
    35521 ctcaccgggg cgcgcgtgtt cttcaagtgc gagaacttcc agcgcatggg ggcattcaag
    35581 ttccgcggag ccttcaatgc cttgtctcaa ctcggacccg aacaaaggca acgtggcgtg
    35641 gtcgcgttct cgtcggggaa ccacgcccag gcggtcgcgc tatccgggaa gatcctcggc
    35701 atcaaaaccg tgatcgtgat gccgctcgac gcgccgcggg tgaagctcga ggcgacacgc
    35761 ggctacggcg ccgaagtcgt gcagtacgcc aagggccagc cgcgcgacga gctcgccgga
    35821 aagctcgcca ccgaacgagg tctcacgctg gtcccgccgt tcgaccatcc gcacatcatc
    35881 gccggccagg gcaccgcggc gaaggagctc gtcgaggacg cggggccgct cgacgtgctg
    35941 ctcgtgccct gcggcggcgg cgggctgctt tcgggctgcg cgatcgcggc gaagcacctc
    36001 gcgccgaaat gccgcgtgct cggcgtcgag ccggaggccg ggaacgacgc ctcgcagtcc
    36061 ttcaaccaga agcggctggt tacgatcgcc attcccgaca ccatcgccga cggcgcgcgc
    36121 acgccctcgc tcggcaagct caccttcgaa atagtgatgc gcaccgtgga cgacatgctc
    36181 accgtcaccg acccggagct gacgcgcgcg atgtttttcc tgtgggagcg cctgaagatc
    36241 gtggtcgagc ctaccggggc cttggccttt gcatcactct ttaccggaaa gttctccgga
    36301 aaaggccgcc gtatcggcgt cgtgctgtca ggcggaaacg tcgaccttcg ttgggcggcc
    36361 gcgcagctgg acaagaacca gggttgacgc accgatcagc gtcgccgcga gcgtgatgtc
    36421 gaagcgcggc gaaaccgcgg ctatcgcgca accgaagagc aggaatcgca gcggccaggc
    36481 gatgcggcgc aagccgaaat atccctgcag cgcgaaggac gcgaacgtgc acatcgcgat
    36541 ggcgaacacc acgcattcca ggatgtagag cacttcgccc tgcaggttga gccccggatt
    36601 gaacaggaaa tagaagggca ggatgaagcc gccgatcgcc agcttgaccg tgtaggggcc
    36661 gagcgccatc ggattgacgc cggcgatcgc accggcggcg aagttggcga ccgccaccgg
    36721 cggcgtgatc gccgacaggc aggcgaaata cagcatgaag aggtgcacct gcatctcggg
    36781 caggttgaac acgccgcgca gcaccggcgc gagcagcgcg gcgcccatga tgtagaccgc
    36841 cggcgtcggc atgcccatgc cgagcagcac gaggaacacc gccgagagaa gcagcgtggg
    36901 gatgagcagg ccgccggaga ccatgttgat gagcagcgtg aacttgccgg cgagcgcggc
    36961 gatctcgatc gcgccgatca ccaccccggc ggcggcgacc gcgccgacca gcggcacgag
    37021 ctgcaggatg gtgtcggtgc agcactcgac gaagcggcgc ggcccgatcg ccgtcggccg
    37081 gtggaaccag gagagcacga tcaccgccgc agtcgcgccc gcggcgacgt agaccggcgt
    37141 gtagccggcc acgagcagcg cgatcagcgc gccgagcggc agcaggtgcc gccagccgct
    37201 cgccagcgcg cgccgcaagg gaacgatgtc ggcttccggc aggcacggct cgttgtgccg
    37261 aaccgcttcg ttgtgcacca gaagatagat cgagtagtag tagagcagcg ccggcaccac
    37321 cgatgcccag caaatggcgg cgtacgaaat gttggtgata tcggacatga tgaaagcgac
    37381 cgcgcccatc accggcggca gcatcgcgcc gccgctcgag gcggtggctt cgatcgcgcc
    37441 ggcgcgcgcc gccggcacgc ccatcttctt catgatcggg atggtgagcg ggccggtggt
    37501 ggcgacgtcc gcggtcggac tgcccgagat cgagccgtac aggccgctcg ccgtgacgca
    37561 cgccttggcg gcgccgccgc gcatgcggcc ggtcacggcg ctcgccagat cgaagaagag
    37621 ctgcccgcct cccgacttct cgtagaagct gccgaacagc acgaacagga aggcgtaggt
    37681 cgccgcgact tccagcggcg cgccgaacac gccgttctcc atgatcgtca tcatctcgat
    37741 gaagtaggtg atgccgaagt tgtcgtggtg cagcgcgccg ggcagccaac gccccaagac
    37801 ggccattgac agaagaatcg aaacgagaga agtgaggccc cagcccaccg agcggcggca
    37861 gagctcgagc accagcgcga gcagcgcgaa gcccgcgaca aggtcggcgg tgttcggctg
    37921 cgagaagccg cggttccagt gcaggaagcg gtcgttgaaa aggatgtaat acagcgcgac
    37981 ggcaagcgag gccgcggcga gcgcgacgtc ggtccaggcg agccgcgagc gcccgcggcg
    38041 cgcggtggtg gtcaggaatg cgatcgggaa ggtgatcgcg atgaagatcg agatgtcgag
    38101 gtggaagtac gccgtgaacg cgcgcgccca cgtggcgaac gccacgagcg gtccctcgag
    38161 agcgaatcgg gaaagccaca gcgcggcgtt gcccgtgatc tgcgcgagcg cgccgatcca
    38221 cagcacgtag agcgcggcgg ccaccgagcc ggccttgatc agcaccgccc agacgccgcc
    38281 cgggcggtgg cggctcccct cccaggtgag taaacccacg ccggcttact tcaccaggcc
    38341 cttttccttg tagtagcgca gcgcgcccgg gtggtacggg agattcgccg gattgaccga
    38401 catcttctgg gtggcgaaag cggtcttgag cgatccgccg atgtcctgca cgcgcttctc
    38461 gttctccgcg atcgccttca cgtacttgta cacgacctcg tcggaaacgt gattacccgc
    38521 cacgatggag atccacatgc gcaccgtcgg atagtccttc tccatgaagg gataggtacc
    38581 cttctccacc atgtcggcct tgaagttgaa ggtgtcggcg gccttctgga tcgtggcgcg
    38641 atcgccgtcg atccagagca gcgcccgctt gccggagacg tccttcacca gcgagtcgcc
    38701 caggttgcgc gcgttcatga agacgtcggc cttgccgtcg acgatcgcgt ccaggccgat
    38761 ctggctgttg cccagcacgt agctgccgcc ccacttctcg atgtcctgga tggtgaagcc
    38821 gtgcgccttc atcacttcgt agggcccgcc gatggtggtc tgcgggttgt cggtgcggtt
    38881 gatggcgagg cgcatcctcg gcttcttcgc cgcgatgtcc gcccacgact tgacgccgtt
    38941 cgcgtccgcc cactccttgg tcatcaggaa gtggatgaac tggttgtcat agagctgcat
    39001 cacgtagctg aacttgccct tgagcggctt ggtaaagggc gcggtgcctt cgttcgccag
    39061 gcggatctcg gtgccggtcg cggtcgcggt gaaatcggcc tgcccctcgg cgaccgcgag
    39121 cacgccgccg ccgggcgagt tcggcttgaa cgacactgcg gagccgggat aggcgtcgcg
    39181 caccaccgcg ttcaggcctt ccaccatggt gcgcggatag ccgctcggcg tcgcgccggt
    39241 catggtcgcg ttgatcggat tcttgtcgtt gaaatcggcc aacgccggcg ccgcgagcgc
    39301 cgcggccaat gccatcagga ctgctttcat ccgtgtttcc tccttttggc tttcttcgct
    39361 tttttggctt tcggtttcgc cttccggtcg gagcggcgcc gcttcagctt cggctgggtg
    39421 cgagcgcgct ttcccggctc cgggaatttc aggatggcgt ccttgaagag ctgcaccagc
    39481 ccgaggaccg cggcagcctg cgaggcatag atcgcctcgg tgcgcaccat gagctcctgc
    39541 tggttgtcgg ggttgtacag ctcggccagg tattttccag tcgcctggtc cagcaggaac
    39601 tcgacgcgaa attgggccac gcaaacctcc tccggttaat tagtatcgca tgccatgcgc
    39661 gcagtcgaga tcaccaaacc gggcgggccc gaggttctca agcccgccga ccgtccgaag
    39721 cccgttccca aggaaaatga aatcctcgtg aaggtcgcgg ccgcgggggt gaaccgcccc
    39781 gacgtcctgc agcgcagcgg caactatccc gttccccccg gtgcttcgga tcttcccggc
    39841 ctggagatcg ccggcgaagt cgtcgggggg tctacaaagc acttcaagcc cggggacaag
    39901 gtctgcgcgc tcgtcgccgg cggcggctat gccgagtact gcgccgtgcc cgaagtgcag
    39961 gcgcttcccg ttcccaaggg cctgacacct gtgcaggccg cgtcgctgcc cgaaacgttc
    40021 ttcactgtct ggtcgaacgt ctacgaccgg gccaagctcg ccccgggcga gtcgctgctg
    40081 gtgcagggcg gcagctccgg catcggcgtc accgccatcc agatggccgc ggcgctcggc
    40141 aaccgcgtgt tcgccaccgc cggctccgac gagaaatgcg cggcctgcct gcgcctcggc
    40201 gcggcggcag cgttcaacta caggactcag gattttgaaa aggagattct cggggcgacg
    40261 ggcggcaagg gcgtgaacgt catcctcgac atggtcggcg gcgactacgt gccgaaggag
    40321 ctcaagtgcc tcgcggagga cggcaggctc gtcttcatcg cgtatctgcg cgggccaaaa
    40381 gcagaagtga acatagacac cgtgatgcgc cgtcggctca ccatcagcgg ctcgacgctg
    40441 cgcccgcggc cgatcgagtt caaaggcgcc atcgcgcaca gcctgcacga gaagatctgg
    40501 ccgctgatcg aatcgggccg catcaagccc gagatctaca agacctttcc tctcgagcag
    40561 gcggcagagg cgcacaagct catggagagc tcgcagcaca tcggcaaaat cgtccttacc
    40621 gtttagcgga ttcggtcttc agccattcca ggaaagcggc gacctccggc cgtcctgccg
    40681 cattccgcga gacgatcgcg aaataggcgc gcgccgggtc ggcctggctc ttgaacggcg
    40741 cgacgagcgc tccctcggca atcaaatcct ttaccagcgg gctgcggccg agggcgacgc
    40801 cgtggccggc gaccgcggcc gggatgatct gctcgtagcc cgagaacgag agcgtgcccg
    40861 cgggcctcag gtccgcgatg cgctcgactt cgagccacgt cttccagtgc agccagggat
    40921 ggcgcacctc ggggtcgtcg tattgcagca gcacgtggtt tctcaggtcc gcgggctttg
    40981 ccagcggctt cttcctgagg agcttggggc tgcacaccgg gaagactttc tcgccgaaca
    41041 ggcggatcgc gttcgggccg gcgagcgacg ccgggccgtg gcgcagcgcg aggtcgagcc
    41101 cgtcgcgctc caggtcctgc accttggtgt cggcggtgat gcgcacgtcg acccccggat
    41161 ggtcgcgcgt gaaacccgcg aggcgcggga tcagccacag cgccgcgaac gaatgcgtgg
    41221 tggtcaccga aagcgccttc ttgccggcat gcgcgcgcag ccggtcggtg gcggtacgca
    41281 tcgtggagag cgcttgcgcc gcggcggcgt agaactggtg gccggcctcg gtgagcgcga
    41341 gcgcgcggtg gcggcgctgg aacaggacca ccccgagctg gtcctcgagt tccttgatct
    41401 ggcggctcac cgccgactgc gtgagatgga gctcctcgcc cgccttggtg aacgacaggt
    41461 ggcgcgccgc cgcctcgaag ccgaccagca ggtccagggc gggcaaagca ttccggttac
    41521 gcatgccaga gctgagaatt taccgtttga gagccgaagc gtaagcatgc atcctgcgca
    41581 taccaaaggc aaggagccga acatgagaat cgaactgagg accggtgagt ttcagctgca
    41641 gcggggccag accctgaagg tggtggacgg cgcgggcagc accatctgcg ccagccaggc
    41701 cgcggtgtgg atcaccgagg aaaaccggcc gcgcgacatc gtggtcgagc ccggcgggtg
    41761 ctaccggctg cgcgagcgtg gcgtcgcggt gatcgaggcg ctcggcaaag cctcggtgtc
    41821 gttcgcgtaa gggagatgcc catgagatac gacttcagct atgtgcacgc ggcgcaagcc
    41881 agggcgcggc aggagcgcgc cgaggcgatc gtcgcctacg ttctgtcgct gttccggagg
    41941 acccgatgaa actgacctac gaagccatcc tgaacgaccc cggcctgctc gagcggctcg
    42001 agaagcaggc gcaccgcgag cgcgcggccg ccgtgcacca gctgatcgtc gaaccgatca
    42061 agcgcgtctt cgccgaccat gccgctcgtc cgcatctcgc tcgccaaggg taagcccgag
    42121 gcctaccggc gcaaggtggg cgacgcggtc caccgtgcgc tggtagaaac cgtcggcgtg
    42181 cccccgctcg accgcttcca gctcctcacc gagcacgagc ccggcgacct ggtctacgac
    42241 tcgaactacc tcggcatcgc gcgctcgagc gatctcgtca tcgtgcagat cacgctgagc
    42301 gccgggcgga ccctcgcgca gaagcgcgcg ctctaccggc gcatcgccgc caacctcgca
    42361 gccgcggttg gactgcggcc gcaagacgcg tggatcaacc tggtcgaggt ggcgaaggag
    42421 aactggtcgt tcggcaacgg cgccgcctcg tacgcaccgg cagaggcgca agccgcgtga
    42481 gcccggccgc tcaggtgctc gagcagatcg acgaggtgct gcagtccctg ggcgcgaaca
    42541 agtcgaatct gctgaccgcg cgcgtgctcc tttccgacgc ggagctgttc ccggactacg
    42601 acaacgcgtg gaacaagtgg ctcgatgcgc tgcgccggcc gctgcgcgta tggcggcccg
    42661 cggaggaaaa ccatgtatga ccacatcgga ttgagagtga aggacctgaa ggcggcggtg
    42721 cgcttctacc agtcggcgct cgagcccctg ggccacatgc tggacgactc gggcgtgggc
    42781 ctcgggccgc ggggacagcc cggcctctgg ctgcatgccg acgggcaacc gggtggcggc
    42841 gcgcacgtcg catttcgcgc cgccgaacgt caggcggtcg acaggttcta cgccgcgggc
    42901 ctcaaggcgg gcgggcgcga taacggcaag ccgggcgtgc gcaccgacta cagccctact
    42961 tattacgccg cgttcctgat cgatccggac gggaacaacg tcgaggccgt ctgcgtgaaa
    43021 tagaatgggg aggtgttcaa gcccgacctc ctgaaaggca agcgcatcct cattaccggc
    43081 ggcggcaccg gcctcggcaa ggaaatcgcc gccaaatacc tcgagctcgg cgccgagctg
    43141 tggatctgcg gccgccgcgg cggggttttg gaccaaaccg cccgggaact ggtctccagg
    43201 catcccggtt cgatcaaaac ccacgctatc gacattcgag acgccgctgc cgtcgacgcg
    43261 atggtgcagc ggatctggga tgaagccggt ccgcttaccg gcctggtcaa caacgcagcc
    43321 ggcaatttca taagcccgac gaaggacctg acgccgaacg gcttcaacgc gattgccaac
    43381 accgtctttc acggcacgtt ctacgtcacg cacgcggtcg gcaagcgctg gatcgccggc
    43441 ggcaacaagg gctcggtcgt ctcgatcctc gtcacctggg tatggaccgg ttcgccgtac
    43501 gtcgtgcctt ccgcgatgtc gaaagcggga ctgcacatca tgacgaagtc gctcgcggtc
    43561 gaatggggca agcacggcat ccgcctgaac gcgatcgcgc cgggaccgtt tccgaccgaa
    43621 ggcgcgacga agcgcctgcg cccgggcgga gatttcggcg aatcaaccaa ggtcaacccg
    43681 atgcgccggg tcggcgagat gtcggagctg cagaacctcg cgacgttcct gatggccgac
    43741 ggctgcgagt ggctctccgg ggagacgatc gcgatcgacg gcgcgggcta cctcgcgacc
    43801 ggcgcgtact tcaccgagct cgacaagctg tccgacgcgg actgggagcg cgcgcgcgcc
    43861 atgatcaagg cgcaaaacga caaggaccgc gctggccgca gcgcatgacg agcgcgccgg
    43921 gcgcaaag
//
LOCUS       DATBSZ010000267        14435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1419378, whole genome shotgun sequence.
ACCESSION   DATBSZ010000267 DATBSZ010000000
VERSION     DATBSZ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14435
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419378"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..97
                     /locus_tag="VIG70_17325"
     tRNA            13..97
                     /locus_tag="VIG70_17325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47..49,aa:Leu,seq:tag)
     gene            114..1412
                     /gene="tig"
                     /locus_tag="VIG70_17330"
     CDS             114..1412
                     /gene="tig"
                     /locus_tag="VIG70_17330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004539679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY8252358.1"
                     /translation="MANVENLGTLERRVSMALAADDIERKIDERLKKLARSVRMPGFR
                     PGKVPLKLIAQTYGPQVRSEVLGDAVQKAFTDAVKEANLKVAGYPKIEKKDPASNTLE
                     FNATFEIYPEVKVGDLANATIERPQLEVDDAAVDKTIQILRKQRTRFEPAGRPARDED
                     RVTVDFKGTIAGEAFPGGEANDFVFAIGAGRMLPEFEAAARGMAPGEQKTFELTFPAD
                     YHGKEVAGKTASFAMTLKKIEEPRLPEVDADFAKALGVVDGDTGKMRTEVRANVEREV
                     KKRVEARTKEQALQALLTAAPLEVPKSLIGMEASSMAERAVADLKARGVNPEQVPINP
                     QAFEEAARRRVALGLVIAELARAESLQPKPAEVRALVEQEAQSYESPAEVVKWFYMQP
                     QRLSEMEALALETNVVKWVLTKAKVLDKPVTFDELMGASS"
     gene            1409..2047
                     /gene="clpP"
                     /locus_tag="VIG70_17335"
     CDS             1409..2047
                     /gene="clpP"
                     /locus_tag="VIG70_17335"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEY8252359.1"
                     /translation="MNWEKGRAEGIVEPSNLGLIPMVIEQSGRGERAYDIYSRLLKER
                     VVFLVGPVTEITANLIVAQLLFLESENPDKDIFFYINSPGGSVSAGLAVYDTMQFIKP
                     DVSTLCVGQAASMGALLMAAGDAGKRFCLPNSRVMIHQPMGGFQGQATDIEIHAKEIL
                     YLRERLNEILARHTKQTIEVIRRDTERDFFLGAEDAVKYGIVDKVLVSRENT"
     gene            2070..3341
                     /gene="clpX"
                     /locus_tag="VIG70_17340"
     CDS             2070..3341
                     /gene="clpX"
                     /locus_tag="VIG70_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY8252360.1"
                     /translation="MSEKSSSGEKLLYCSFCGKSQHEVRKLIAGPSVFICDECIELCN
                     DIIREETASDKGGKGAKSDLPTPREIRNILDSYVIGQDQAKKILSVAVYNHYKRLKHL
                     SKNDEVELAKSNILLIGPTGSGKTLLAQTLARLLNVPFVIADATTLTEAGYVGEDVEN
                     IIQKLLQNCDYDVDKAKRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     IASVPPQGGRKHPNQDFVQVDTTNILFVCGGAFDGLEKIVRNRTEKTGIGFGAEVRSP
                     KNSKATYDLLKNVEPEDLIKYGLIPEFVGRLPVLATLEELDESALIQILTEPKNALIK
                     QYQRLFHMEGVELEVRTTALGAVAKRALARKTGARGLRSILEQILLDTMYELPTLENV
                     SKVVVDESMVSADGKPLLIYSDNQKVAGGAG"
     gene            3497..5926
                     /gene="lon"
                     /locus_tag="VIG70_17345"
     CDS             3497..5926
                     /gene="lon"
                     /locus_tag="VIG70_17345"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003267809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY8252361.1"
                     /translation="MSETKPPSAEIIQYPLLPLRDVVVFPHMVIPLFVGRPKSIKAME
                     TAMEAGKSILLVAQRSAAKDDPGPEDMYSIGCVSNILQMLKLPDGTVKVLVEGGTRAQ
                     IREVVDVRTHWVAEATAVPADGAPPAEVEAMRRALLSAFDQYVKLNKKIPPEILTSLS
                     GIDEAGRLADTVAAHLPLKLEQKQQVLEMFEVKQRLEHLLSQLETEIDILQVEKRIRG
                     RVKRQMEKSQREYYLNEQVKAIQKELGEGEEGADLEEMEKRIKRAHMPKDARKKAEAE
                     LKKLKLMSPMSAEATVVRNYIETIVNLPWKKKSKISKDIKLAQEILDKDHYGLEKVKE
                     RIVEYLAVQTRVDKMKAPILCLVGAPGVGKTSLGQSIARATNRKFVRMSLGGVRDEAE
                     IRGHRRTYIGSMPGKILQNMSKVQVKNPLFLLDEVDKMGADWRGDPSSALLEVLDPEQ
                     NNTFTDHYIEVEYDLSEVMFVATANTLNIPPPLLDRMEVIRLSGYTEDEKMNIAVRYL
                     LPKQLKATGLQEGELSVSEAAVRDIVRYYTREAGVRAMERELSKICRKAVKQLLTERQ
                     SAPAGKPHRITVTPKNLDKFLGVRRYTFGMAEKQNQVGQVTGLAWTEVGGELLTIEAV
                     TMPGKGKTITTGKLGDVMQESIQAALSVVRARSRKLGVKEDFYQKNDIHIHLPEGATP
                     KDGPSAGVGIVTSMVSILSGIPVRSDVAMTGEITLRGEVLQIGGLKEKLLAAHRGGIK
                     TVLIPEENVKDLTEIPDNIKNKLEIVPVKWIDKVLEVALERQPEPLPEHDESAPIPSK
                     PDDQAGVVRPH"
     gene            5937..6209
                     /locus_tag="VIG70_17350"
     CDS             5937..6209
                     /locus_tag="VIG70_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY8252362.1"
                     /translation="MNKADLIDQIAQAAEISKSAAERAVDALVGAVKSSLRKDEMVTL
                     VGFGTFYAGNRAARTGRNPRTNEPIEIRAARLPKFRAGKALKDAIN"
     gene            6251..6325
                     /locus_tag="VIG70_17355"
     tRNA            6251..6325
                     /locus_tag="VIG70_17355"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6283..6285,aa:Val,seq:tac)
     gene            6346..6422
                     /locus_tag="VIG70_17360"
     tRNA            6346..6422
                     /locus_tag="VIG70_17360"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6380..6382,aa:Asp,seq:gtc)
     gene            6598..7497
                     /locus_tag="VIG70_17365"
     CDS             6598..7497
                     /locus_tag="VIG70_17365"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8252363.1"
                     /translation="MAGKRLKTVLFAEVSGRDEFVRAAGPVMGWNGISDCVDRLQRTA
                     RSSGGRVVKVIGSELMMLFESPDAAAGAASRMHAAVNALPSVSGTRLSVKVGYHAGPV
                     VEGNDDLLGDTVKLVAALLRQAQAGQTLTTHQTAQLLNDQSRSYSGQVYAPSHAAGGN
                     RERPRAMPARSYSGQAHAAPLVAGGTRERPRAQRMLRLSYGSESVTCGLDNEAVVVGR
                     DPTCQLVAATAFASRRHCSILLRPDGFKILDHSSNGTFLTNEGGEEIELLNGQEAPLA
                     RRGWVSLGCSRAFAIRLLEFTGH"
     gene            complement(7517..8038)
                     /locus_tag="VIG70_17370"
     CDS             complement(7517..8038)
                     /locus_tag="VIG70_17370"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8252364.1"
                     /translation="MPRLPCLISLGLCLFVPPALARDVPFLPTPEAVVEKMLEVAKVG
                     PNDVVYDLGSGDGRIVIAAAKKRGARGVGIDIDPDRIREARDNAKRAGVSNRVEFREQ
                     DLFTANFSEATVVTMYLLSSVNLKLRPKLLAELRPGTRIVSHAFHMGEWRPLETHWVG
                     SSAVYYWVVPELK"
     gene            8132..9982
                     /locus_tag="VIG70_17375"
     CDS             8132..9982
                     /locus_tag="VIG70_17375"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY8252365.1"
                     /translation="MFDLVHKYRRVVQVILALLVIPFAIWGVESYTQFRGGRDAVATV
                     NGMEISQRQFGEEMRRQQEQMRRMFGGTIDPAMLDSPDSRRAVLEGLIGQRLLAAEAA
                     RAHMFMSREAVIEAITSAPEFQEGGKFSTAKYSAYLASRGLSDQGNVAELQSQIPVAR
                     FVGSISETAIAPRSVAARLAALEAQKREVSEARIAEQQFLPQVKIDDAQVKAHYDANQ
                     ADYRTPERVRAEYVVLSAEALARQDPPTEAEVKSAYEARASQFRVEEQRRASHILVKA
                     KEEAEKIFQEAKKAPGRFAELAKKHSQDPGSAEKGGDLGWFGRGMMVKPFEDAVFKLG
                     QNEMQLVESEFGFHIVRLTGVQAAKARPYEEARKELADDLARQKGQRRFAEAAETFNN
                     LVYEQSDSLKPAAERFKLQIQQTGWIAKTARQELGALDNPKLLAALFSTDTLKNRRNT
                     DAIEVAPSTLVAARVLEHQPAAQRDFAEMKGAIAEQLRRREASALALKDGMTRLEKLR
                     KGEDAGVKWGLPRLVSRRDAQGLPINILRQVVSADTSKLPAYVGIPIPDGGYSLIRIS
                     KVIEEPAKEGDPQVAERAAQLYGGAQYEAFVESLRSRAEIEINRKNLEAK"
     gene            complement(9954..10769)
                     /gene="fabI"
                     /locus_tag="VIG70_17380"
     CDS             complement(9954..10769)
                     /gene="fabI"
                     /locus_tag="VIG70_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY8252366.1"
                     /translation="MALLSGKKILVTGVLSNRSIAYGVAKAARREGAELALTYQNERF
                     KDRVTEMAKELGSSIALPCEVTSDDEIRNLFAQLGERWQTLDGLVHAIAFAPREAIAG
                     DFLEGISREAFRQAHDISAYSFAALAKAALPLLHKNSSLLTLTYLGAARVVPNYNTMG
                     LAKASLEAAVRYLAASLGPQGIRVNGISAGPIKTLAAAGISGFGKILKFVEEHAPLRR
                     NVTTEDVGNAAAFLLSDLAAAVTGEILYVDAGFSHVVAGISSERATSPPDSSG"
     gene            10792..12417
                     /locus_tag="VIG70_17385"
     CDS             10792..12417
                     /locus_tag="VIG70_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8252367.1"
                     /translation="MRHLVVALSFWVAGAQAADPSKILRITFQAAETGFDPVKVSDYY
                     SGTVIEAIFDPLLTYDYLARPATLVPNTAVSLPEITDQGRTYTLRVKPGITFADDAAF
                     KGVKRELTAADYAYSIGRFLDPKNRSPYAFLYEGVDRLEAPERYTLRIRLKNPDLNFG
                     HVLAFSLSGAVARDAIEAYGDESAAHPVGTGPFRLKRYVRSSKIVLEANPGYREVLRE
                     GKRLPLIGGVEISIMEETQSRWLAFQRGETDMEYQLAEVAPTFITPGGELKPEFAKRG
                     IKLERSVDPEIIYLYFNMQDKTVGGLSKEKIALRRAIAMAYKVEDQIRIIRKGQSIRA
                     QYPIPPGVAGHDPEYRSSIAHDPRAANALLDRFGYKKGPDGYRRMPKGEPLVIRYSST
                     PSERDRQFDELMKRSLDSIAVRLEIHKDRFPELIKLENQCRLMMRGAAWIADYPDGDN
                     FMQLLYGPNSGQSNNACYRSAEYDKRYEKSRLLPHGPERDRLYREMTRIMETDTVWLL
                     TDSRYRNVLLQPRVKGFKKHPVMHAEWLYLDLE"
     gene            12410..13348
                     /locus_tag="VIG70_17390"
     CDS             12410..13348
                     /locus_tag="VIG70_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8252368.1"
                     /translation="MSSYVARRLWQFVPTLLGVVLLVFFLFNWVGGDPAYILAGKIAN
                     PESIENIRRSLGVDQPYWVQLWIFIKQIAVADFGASWSTNERVSQIFATRLGPSLTVL
                     LPLLVISTILALAAAMLVAYYRGSLTDRAIMIGCTVGQSVSILVYILVLQYLLAYQLG
                     WFPVQGWGSTFTDSLLVYAALPILVGVVVSLAPDIRLYRSFFLEEMGQDYVRTARAKG
                     LGEGRVMWLHVLRNAAIPIVTNVMTQVPGLLAGAFLIERFFSIPGIGREVILAVERSD
                     FPVIKAVTVYVAIATMVINLAADLLYRTLDPRVQLK"
     gene            13359..14351
                     /locus_tag="VIG70_17395"
     CDS             13359..14351
                     /locus_tag="VIG70_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8252369.1"
                     /translation="MSLWRLAWRRFLRDRVGLVSLVIVIAFVALACASAAGWVARDWA
                     EEAGVSYALPRFLGSQVEEAAAQGAATVGPQETHGIPDPLAEELAEIQKDLLGLSGTA
                     SRAGTLVFGGDKWGRDVVKKAIKGTETSLVVGLAAAALAAFLGTLFGAAAGYYGRWVD
                     DFFNWLYSVFTSIPYLLLVLAVAAVLNQKGTVTVILILGLTGWTGIFRLIRAEYLKHK
                     VREYVLAADAIGASDARRMFAHILPNVSHVVLVQLSLHVVLFIKSEVILSFLGFGVGV
                     DTVSWGSMLAEAPSELILGMWWQVAAATLAMALAVTAFSLFTDSVRDALDPRLQ"
BASE COUNT         2718 a         4613 c         4774 g         2330 t
ORIGIN      
        1 gttgattatt cggcgaaggt ggcggaattg gcagacgcac cagacttagg atctgacgcc
       61 gcaaggcatg agggttcgag tccctccctt cgcaccagga cctaatcgag aaaatggcga
      121 acgtggaaaa cctaggaacg ctggagcggc gcgtatcgat ggcgctggcg gccgacgata
      181 tcgagcgcaa gatcgacgag cggctgaaga agctcgcgcg cagcgtgcgc atgccgggat
      241 tccggccggg gaaggtgccg ctcaagctga tcgcgcagac ctacggcccg caggtgaggt
      301 ccgaagtgct cggcgacgca gtgcagaagg cgttcaccga cgcggtgaag gaagccaacc
      361 tgaaggtggc aggctatccc aagatagaaa agaaggatcc ggcttcgaac accctcgaat
      421 tcaacgcgac gttcgagatt tatcccgagg taaaggtcgg agacctggcg aacgcgacga
      481 tcgagcgccc gcaactcgag gtcgacgacg cggcggtcga caagacgatc cagatcctgc
      541 gcaagcagcg cacgcgcttc gagccggccg ggcgcccggc gcgagacgaa gacagggtga
      601 ccgtggattt caagggaacg atcgccggcg aggctttccc cggcggcgag gccaacgact
      661 tcgtgttcgc gattggcgcc ggccggatgc tgcccgagtt cgaggcggcg gcgcgcggca
      721 tggcgcccgg cgagcaaaag actttcgagc tgacgttccc cgccgactac catggcaagg
      781 aagttgctgg caaaacagcg tcgttcgcca tgacgctgaa gaagatcgag gagccgcgcc
      841 tccccgaagt cgacgccgat ttcgcgaagg cgctcggcgt ggtggacggc gacaccggca
      901 agatgcggac cgaggtgcgc gcgaatgtcg agcgcgaggt gaaaaaacgc gtcgaggcgc
      961 ggaccaagga gcaggcgctg caggcgctgc tcacagccgc gccgctcgaa gtgccgaaat
     1021 cgctcatcgg catggaggcg agcagcatgg ccgagcgcgc ggtggcagac ctcaaggcgc
     1081 gcggggtcaa tccggagcag gtgccgatca atccgcaggc gttcgaagaa gcggcgcggc
     1141 ggcgcgtcgc gctcggcctg gtgatcgcgg agctcgcgcg cgccgaaagc ctgcagccca
     1201 agcccgccga agtgcgcgcg ctggtcgagc aggaggcgca aagctacgag agtccggccg
     1261 aggtagtaaa atggttctac atgcagccgc agcggctttc agagatggaa gcactcgcgc
     1321 tggaaaccaa cgtcgtcaag tgggttttga cgaaggcgaa agtgctcgac aagccggtaa
     1381 cgtttgacga gctgatgggg gcgagctcgt gaactgggaa aaaggaaggg cggagggaat
     1441 cgtcgaaccc tcgaacctcg ggctgatccc gatggtcatc gagcagtcgg ggcgcggcga
     1501 gcgcgcctac gacatctatt cccggctgtt gaaggaaagg gtggtgttcc tggtggggcc
     1561 ggtgaccgag atcaccgcca acctgatcgt ggcgcagctc ctgttcctcg agtcggagaa
     1621 tcccgacaag gacattttct tctacatcaa ttcgcccggc ggttcggtgt cggcgggcct
     1681 ggccgtctac gacaccatgc agttcatcaa gcccgacgtg tcgacgctgt gcgtcggcca
     1741 ggcggcgagc atgggcgcgc tgctcatggc cgccggggac gcgggcaagc gcttctgcct
     1801 gcccaactcg cgcgtcatga tccaccagcc catgggcgga ttccagggcc aggccaccga
     1861 catcgagatc cacgccaagg agatcctcta cctgcgcgag cgcctgaacg agatcctggc
     1921 gcggcacacg aagcagacga tcgaggtgat ccggcgcgac accgagcgtg actttttcct
     1981 cggggccgaa gacgcggtaa agtacggtat cgtggacaag gtgctggtgt cacgggagaa
     2041 cacgtgaaga aaagggggct cacgtagcaa tgtcggaaaa atcctcctcg ggggaaaagc
     2101 ttctctactg ctcgttctgc ggcaagagcc agcacgaggt gcgcaagctg atcgccggcc
     2161 cgtcggtgtt catctgcgac gaatgcattg agctgtgcaa cgacatcatc cgcgaggaga
     2221 ccgcctccga taagggcggc aagggcgcca agtcggacct gccgacgccg cgcgagatcc
     2281 gcaacatcct ggacagctat gtcatcggcc aggaccaggc gaagaagatc ctctcggtcg
     2341 cggtctacaa ccactacaag cggctcaagc acctctccaa gaacgacgag gtcgagctcg
     2401 cgaaatccaa catcctgctg atcgggccga cgggctccgg aaagacgttg ctcgcccaaa
     2461 cattggcaag gttattgaat gttccttttg tcatcgcaga cgcgaccacc ctcaccgagg
     2521 cgggctacgt cggcgaggac gtcgagaaca tcatccagaa gctgctgcag aactgcgact
     2581 acgacgtcga caaggcgaag cgcggcatcg tctacatcga cgagatcgac aagatctcgc
     2641 gcaagtcgga caatccctcg atcacgcgcg acgtgtcggg cgagggcgtg cagcaggcgc
     2701 tgctgaaact gatcgagggc accatcgcct cggtgccgcc gcagggcggg cgcaagcacc
     2761 cgaaccagga cttcgtccag gtcgacacca ccaacatcct gttcgtgtgc ggcggcgcgt
     2821 tcgacggcct ggagaagatc gtgcgcaacc gcacggagaa gaccggcatc ggctttggcg
     2881 cggaagtgag aagcccgaag aattccaaag cgacctacga tctgctcaag aacgtcgagc
     2941 ccgaggacct gatcaagtac gggctgatcc cggagttcgt gggacgcctc cccgttctcg
     3001 ccacgctgga agagctcgac gaaagcgcgc tgatccagat cctgaccgaa ccgaagaacg
     3061 cgctcatcaa gcagtatcag cgcttgttcc acatggaagg cgtggagctc gaggtgcgca
     3121 cgacggcgct cggcgccgtg gcgaagcgcg ccctggcacg aaagaccggc gcaaggggcc
     3181 tgcgctcgat cctcgagcag atcctgctcg acacgatgta cgagttgccc accctcgaga
     3241 acgtcagcaa ggtcgtggtg gacgaatcga tggtcagcgc cgacggcaag ccgctgctca
     3301 tctattcgga taaccagaag gtcgccggcg gcgctggtta gtcgtttttc cccaaatttc
     3361 gtcctaaagc cctacattta gtaacgcttt ccgcgttgca gcgtaaagct gttgcgtgta
     3421 cgcttttcct cattgatttt tcgggaggaa cccgcatatc accgtggggt cccaacccat
     3481 tgaaaggctg atctaaatgt ccgaaacaaa gcctcccagc gccgaaatca tccaataccc
     3541 gctgctgccg ttgcgggatg tggtggtgtt tccccacatg gtgattccgc tcttcgtcgg
     3601 ccggcccaag tcgatcaagg caatggagac cgccatggag gcgggcaagt cgatcctgct
     3661 ggtggcgcag cgctccgcgg ccaaggatga ccccggccct gaggacatgt attccatcgg
     3721 gtgcgtgtcg aacatcctgc agatgctcaa gctgcccgac ggcacggtga aagtgctggt
     3781 cgaaggcggt acccgcgcgc agatccgcga agtggtggac gtgcgcacgc actgggtcgc
     3841 cgaggcgacc gcggtgccgg ccgatggcgc gccgccggcc gaagtcgagg cgatgcgccg
     3901 cgccctgctc tcggcgttcg accagtacgt caagctgaac aagaagatcc cgccggaaat
     3961 cctcacttcc ctttccggca tcgacgaggc cggccgcctt gccgacaccg tcgccgcgca
     4021 cctgccgctc aagctcgagc agaagcagca ggtgctggag atgttcgagg tgaagcagcg
     4081 cctcgagcac ctgctgtcgc agctcgagac cgagatcgac atcctgcagg tggagaagcg
     4141 catccgcggc cgggtcaagc ggcagatgga gaagagccag cgcgagtact acctgaacga
     4201 gcaggtgaag gcgatccaga aggagctcgg cgagggcgag gagggcgcgg acctcgagga
     4261 gatggagaag cgcatcaagc gcgcgcacat gcccaaggac gcgcgcaaga aggcagaggc
     4321 cgagctgaag aagctcaagc tcatgtcgcc gatgtcggcc gaggcgaccg tggtgcgcaa
     4381 ctacatcgag accatcgtca acctgccgtg gaagaagaag tcgaagatct cgaaggacat
     4441 caagctcgcg caggagatcc tggacaagga ccactacggg ctggagaagg tcaaggagcg
     4501 catcgtcgag tacctggcgg tgcagacgcg cgtggacaag atgaaggcgc ccatcctctg
     4561 tctcgtcggc gcgcccggcg tcggcaagac ttcgctcggc cagtcgatcg cgagggcgac
     4621 caaccgcaag ttcgtgcgca tgtcgctcgg cggcgtgagg gatgaagccg agatccgcgg
     4681 ccaccgcagg acctacatcg gctcgatgcc gggcaagatc ctgcagaaca tgtcgaaggt
     4741 gcaggtcaag aacccgctgt tcctgctcga tgaagtcgac aagatgggcg ccgactggcg
     4801 cggcgatccg tcgtccgcgc tgctcgaggt gctcgacccg gagcagaaca acactttcac
     4861 cgaccactac atcgaggtgg agtacgacct gtcggaggtg atgttcgtcg ccaccgctaa
     4921 cacgctcaac attccgccgc cgctgctcga tcgcatggaa gtgatccgtc tctccggcta
     4981 cacggaggac gagaagatga acatcgccgt gcgctacctg ctgccgaagc agctcaaggc
     5041 caccgggctc caggaaggtg agctgtccgt gtccgaggcg gcggtgcgcg acatcgtgcg
     5101 ctattacacc cgcgaggccg gcgtgcgggc gatggagcgc gagctctcga agatctgccg
     5161 caaggccgtg aagcagctcc tcaccgagcg gcagagcgcg cccgcaggca agccgcaccg
     5221 catcaccgtc acgcccaaga atctcgacaa gttcctcggc gtgcggcgct acaccttcgg
     5281 catggccgag aagcagaacc aggtcggcca ggtgaccgga ctcgcctgga ccgaggtcgg
     5341 cggcgagctc ctcacgatcg aggcggtcac catgcccggc aagggcaaga ccatcaccac
     5401 cggcaagctc ggcgacgtga tgcaggagtc gatccaggcc gcgctctcgg tggtgcgggc
     5461 gcgctcgcgc aagctcggcg tgaaggaaga cttctaccag aagaacgaca tccacattca
     5521 cctgcccgag ggcgcgacgc ccaaggacgg cccgagcgcg ggcgtcggca tcgtcacctc
     5581 catggtgtcg atcctgtcgg gaatcccggt gcgctcggac gtcgccatga ccggcgagat
     5641 cacgctgcgc ggcgaggtgc tgcagatcgg cggcctgaag gagaagctgc tggcggcgca
     5701 ccgcggcggc atcaagaccg tgctgatccc ggaggagaac gtcaaggacc tcaccgagat
     5761 ccccgacaac atcaagaaca agctcgagat cgtgccggtg aagtggatcg acaaggtgct
     5821 ggaagtggcg ctcgagcgtc agccggagcc gctgccggag cacgacgagt cggcgccgat
     5881 cccgtcgaag cccgacgacc aggctggagt ggtccggcct cactaggggt ttctttttga
     5941 acaaagcgga cctgatcgac cagatcgcgc aggcggccga gatttcgaag tcggccgccg
     6001 aacgcgcagt cgatgcgctg gtcggcgccg tgaaatcgag cctgcgcaag gacgagatgg
     6061 tgaccctggt cgggttcggc accttctacg ccggcaatcg cgccgcgcgc accggccgca
     6121 acccgcgcac caacgaaccg atcgagatca gagcagcgcg gctgcccaag tttcgtgctg
     6181 gaaaggcgct gaaggacgct ataaactagg cgcccttttt tcaggagcga agaagctcgc
     6241 tgaaaagcac gggtgcttag ctcagcggta gagcgctgcc cttacaaggc agatgtcgta
     6301 ggttcgaccc ctacagcacc caccaggtcg cttgcagcac ggtaaggagc ggtagttcag
     6361 ttggttagaa taccggcctg tcacgccggg ggtcgcgggt tcgagtcccg tccgctccgc
     6421 caacctctag ttcccagcag ttccccctcc gcgtccgcgg catttgtctc actcggcgaa
     6481 cattagttgg ccgagcgatc atttttacct aacagtcctg caccaccgcg gaggaaatta
     6541 aggctgcggg gttgccccgc ggactacgag aggtgtcttg tagcgggcac tatggcagtg
     6601 gcgggaaaga ggctgaagac agtcctgttc gccgaagtga gcggcaggga tgaattcgtg
     6661 cgcgcagccg ggcccgtcat ggggtggaac ggtatttcgg actgcgtcga ccgcctgcag
     6721 cgaaccgccc gctcgtccgg cggcagagtg gtgaaagtca tcggcagcga attgatgatg
     6781 ctgttcgagt ccccggatgc ggctgcagga gcggcctcca ggatgcacgc ggccgtaaat
     6841 gcgctgccct cggtgtccgg aaccaggctc tcggtcaagg tcggctatca cgccggtccg
     6901 gtggtcgagg ggaacgacga tcttctcggc gacacggtca agctcgtggc ggcgctgctg
     6961 cgtcaggcgc aagccgggca gacgttgacc acgcaccaga cggcgcaact gctcaacgac
     7021 caatcgcgtt cctattcggg acaggtttac gcaccatctc acgcggccgg cggcaaccgc
     7081 gagcggccgc gtgccatgcc ggcgcgttcc tattcagggc aggctcacgc agcacctctc
     7141 gtggccggcg gcacccgcga gcggccgcgt gcgcagcgca tgctgcgcct cagctacggg
     7201 agcgaatcgg tgacctgcgg gctagacaac gaagccgtcg tggtgggcag ggaccccacg
     7261 tgccagctgg tggcggccac cgccttcgcc tcgcgccggc actgttcgat cctgttgcgg
     7321 cccgacggat tcaagatcct cgaccacagc agcaacggca ccttcctcac caacgaaggc
     7381 ggcgaagaga tcgagctgct gaacggacag gaggcgccgc tcgcgaggcg cggctgggtg
     7441 tcgctgggct gctcgcgcgc gttcgccatc cggctcctgg agttcaccgg ccactagccg
     7501 gaccctccaa gcctcgtcac ttcaactcgg gcacgaccca gtagtagacg gcgctgctgc
     7561 cgacccaatg cgtctccagc ggcctccact ctcccatgtg gaacgcatgc gagacgatgc
     7621 gcgtgcccgg cctgagctcg gctagcagct tgggccgcag cttcaggttc acgctggaca
     7681 gcaagtacat cgtcactacg gtggcctcgc tgaagttggc cgtgaagagg tcttgctcgc
     7741 ggaattcgac gcgattggag acgcccgcgc gtttggcatt gtcccgtgct tcccggatgc
     7801 ggtcggggtc gatgtcgatg ccgacgccgc gggcgccgcg cttcttggct gcggcgatga
     7861 cgatgcggcc gtcgcccgag ccgaggtcgt agaccacgtc gttggggccg accttggcaa
     7921 cttcaagcat cttttccact acggcctcgg gcgtcgggag gaacggcacg tcgcgcgcga
     7981 gcgccggcgg tacgaaaagg caaaggccga gggaaatcag gcacggcaga cggggcacgg
     8041 cggctccttg aggaagcatg aactctactg cccagccggt ctcccgtgca tcctccggta
     8101 gaattccgcc ttcctcccaa ggtagccgag cgtgttcgat ctcgtccaca aataccggcg
     8161 cgtcgtccag gtgatcctgg cgttgctcgt gattcccttc gcgatctggg gcgtggagtc
     8221 ctacacgcag ttccgcggcg ggcgcgacgc ggtggcgacc gtgaacggga tggagatctc
     8281 gcagcgccag ttcggcgagg agatgcgccg ccagcaggag cagatgcgcc gcatgttcgg
     8341 cggcacgatc gacccggcga tgctcgattc gcccgactcg cggcgcgcgg tgctcgaggg
     8401 gctgatcggc cagcgcctgc tcgccgccga ggcggcgcgc gcgcacatgt tcatgagccg
     8461 cgaggcggtg atcgaggcga tcaccagcgc gcccgagttc caggagggcg gcaagttctc
     8521 gaccgccaag tattcggcgt atctcgcgag ccgcggcctt tccgaccagg gcaacgtggc
     8581 cgagctgcag agccagatcc cggtcgcgcg cttcgtcggc tcgatctccg agaccgcgat
     8641 cgcgccgcgc tcggtcgccg cgcgcctcgc cgcgctcgag gcgcagaagc gcgaggtgtc
     8701 ggaggcgcgc atcgcggagc agcagttcct gccgcaggtg aagatcgacg acgcccaggt
     8761 caaggcgcat tacgacgcca accaggccga ctaccgcacg ccggagcgcg tgcgcgcgga
     8821 gtacgtcgtg ctgtcggctg aggcactcgc gcggcaggac cccccgaccg aagccgaggt
     8881 caagtccgcg tacgaggcgc gcgccagcca gttccgcgtc gaggagcagc gccgcgcgag
     8941 ccacatcctg gtgaaggcga aggaggaggc cgagaagatt tttcaggaag cgaagaaggc
     9001 gccgggccgc ttcgcggagc tcgccaagaa gcattcccag gacccgggct cggcggagaa
     9061 gggcggcgat ctcggctggt tcggccgcgg catgatggtg aagccgttcg aggacgcggt
     9121 gttcaagctc gggcagaacg agatgcagct ggtggaaagc gaattcggct tccacatcgt
     9181 gcgcctgacg ggcgtccagg cggcgaaggc gcggccgtac gaagaggcgc gcaaggaact
     9241 cgccgacgac ctcgcgcgcc agaaaggcca gcgcaggttc gccgaggcgg ccgagacgtt
     9301 caacaacctg gtctatgagc agtcggacag cctgaagccg gccgccgagc gcttcaagct
     9361 gcagatccag cagaccggct ggattgccaa gacggcgcgc caggagctcg gcgcgctcga
     9421 caatccgaag ctgctggcgg cgctcttctc caccgatacc ctgaagaacc gcaggaatac
     9481 cgatgccatc gaggtggcgc ccagcaccct ggtcgcggca cgcgtcctcg agcaccagcc
     9541 ggcggcgcag cgcgacttcg ccgagatgaa aggcgcgatc gccgagcagc tgcgccggcg
     9601 cgaggcctca gcgcttgccc tcaaggacgg catgaccagg ctcgaaaagc tgcgcaaagg
     9661 ggaggatgcg ggggtgaaat gggggctacc gcggctggtt tcgcggcgcg acgcgcaagg
     9721 cctgccgatc aacatcctgc gccaggtggt ctcggccgac accagcaagc tgcccgccta
     9781 cgtggggatc ccgatccctg acggcggcta ttccctgatc cgcatttcga aggtgatcga
     9841 ggagcccgcg aaggaaggcg atccccaggt tgcggagcgc gccgcgcagc tctacggcgg
     9901 tgcgcagtac gaggctttcg tcgagagcct gcgcagccgc gccgagatcg aaatcaaccg
     9961 gaagaatctg gaggcgaagt agcccgctcg gaggaaatgc cggccaccac gtggctgaag
    10021 ccggcgtcga cgtacaggat ctctcccgtc acggcggccg ccaggtcgga cagcaggaag
    10081 gccgcggcat tgcctacgtc ctcggtcgtg acattgcgcc gcaagggcgc gtgctcctcg
    10141 acgaacttga ggatcttgcc gaagcccgag atccccgccg ccgcgagcgt cttgatcggc
    10201 cctgcggaga tgccgttcac ccggatcccc tgcggcccga ggctcgccgc gaggtagcgc
    10261 accgcagcct cgaggctcgc cttggcgagc cccatcgtgt tgtagttcgg cacgacccgc
    10321 gccgcgccga ggtaggtcag ggtcaacagg gaagaattct tgtggaggag aggtagcgct
    10381 gcctttgcga gcgcggcgaa gctgtaggcc gagatgtcgt gcgcctggcg gaacgcctcc
    10441 cggctgattc cctcgaggaa gtcgccggcg atcgcctcgc gcggggcgaa cgcgatggcg
    10501 tggacgaggc catcgagggt ttgccacctc tcacccaact gcgcgaatag attcctgatt
    10561 tcgtcgtcgc tggtcacctc gcaaggcagc gcgatgctcg agccgagctc cttcgccatc
    10621 tcggtgacgc gatccttgaa gcgctcgttc tgataggtga gcgcgagttc cgcgccttcg
    10681 cggcgcgcgg ccttggcgac cccgtaagcg atggagcggt tgctgagcac gccggtgacg
    10741 aggatcttct tgccagagag aagggccatc gtccgattat actttccgcg cgtgagacac
    10801 ctcgtcgtcg cactctcgtt ctgggttgct ggtgctcagg ccgcggatcc ctccaagatc
    10861 ctgcgtatta ccttccaggc ggcggaaacc ggattcgatc cggtcaaggt ctccgactac
    10921 tactccggca cggtgatcga agcgatcttc gatccgctgc tgacctacga ctaccttgcg
    10981 cggcccgcga cgctggtgcc gaacacggcg gtgtccttgc ccgaaattac ggatcagggc
    11041 cgcacctata ccctcagggt caagccgggg atcaccttcg ccgacgatgc ggcgttcaag
    11101 ggcgtgaagc gcgagcttac tgcggccgac tacgcctact cgatcggccg cttcctcgat
    11161 ccgaagaacc gctcgccgta cgccttcctg tacgagggcg tggacaggct cgaggcgccc
    11221 gagcgctaca ccctgcgcat ccggctgaag aatccggatc tcaatttcgg ccacgtcctc
    11281 gcgttctcgc tgtcgggcgc cgtggcacgc gacgcgatcg aggcgtacgg cgacgaatcc
    11341 gccgcgcacc cggtcggcac cgggcccttc cgcctcaagc gctacgtgcg ctcctccaag
    11401 atcgtgctcg aggcaaaccc cggctaccgc gaggtgctgc gcgagggcaa gcgcctgccg
    11461 ctgatcggcg gcgtggagat cagcatcatg gaggagacac agagccgctg gctcgctttc
    11521 cagcgcggcg aaaccgacat ggagtaccag ctcgccgagg tcgcgccgac gttcatcacg
    11581 cccggtggcg agctcaagcc cgagttcgcg aagcgcggca taaagctcga gaggagcgtc
    11641 gatcccgaga tcatctacct ctacttcaac atgcaggaca agaccgtggg gggcctctcc
    11701 aaggaaaaga tcgcgctgcg ccgcgcgatc gccatggcct acaaggtgga ggaccagatc
    11761 cgcatcatcc gcaaaggcca gtcgatccgc gcgcaatacc ccatcccgcc gggcgtcgcc
    11821 ggccacgacc cggagtaccg ctcgagcatc gcgcacgacc cgcgcgccgc caacgcgctg
    11881 ctcgaccgct tcggctacaa gaaaggtccc gacgggtacc ggcgcatgcc gaaaggcgag
    11941 ccgctggtga tccgctactc ctccacgccg agcgagcgcg accgccagtt cgacgagctg
    12001 atgaagcgct cgctcgactc gatcgccgtg cgtctcgaga ttcacaagga ccgcttcccc
    12061 gagctgatca agctcgagaa ccagtgccgg ctgatgatgc gcggcgccgc ctggattgcc
    12121 gactaccccg atggcgacaa cttcatgcag ctcctctacg ggccgaacag cggccagagc
    12181 aacaacgcgt gctaccgctc ggcggaatac gacaagcgct acgagaagtc ccggctcctg
    12241 ccgcacggcc cggagcgcga ccgcctgtac cgcgagatga cgcgcatcat ggaaaccgac
    12301 accgtctggc tcctcaccga cagccgctac cgaaacgtcc tgctccagcc ccgcgtcaag
    12361 ggattcaaga agcacccggt gatgcacgcc gagtggctct acctcgatct tgagtagtta
    12421 cgtcgcccgg agactgtggc aattcgtgcc gacgctgctc ggcgtcgtgc tgctcgtgtt
    12481 cttcctgttc aactgggtcg gcggcgaccc tgcgtacatc ctggcgggca agatcgccaa
    12541 tcccgagagc atcgagaaca tccggcgcag cctcggagtc gaccagccct attgggtgca
    12601 gctctggatc ttcatcaagc agatcgcggt cgccgacttc ggcgcgagct ggtccaccaa
    12661 cgagcgggtc tcgcagatct tcgcgactcg cctggggccg tcgctgacgg tgcttttgcc
    12721 tttgttggta ataagcacaa ttcttgccct ggcagccgcc atgctggttg cttattaccg
    12781 cggctcgctc accgaccgcg cgatcatgat cggctgcacc gtcgggcagt ccgtcagcat
    12841 cctggtctac atcctcgtcc tccagtacct gctcgcctat cagctcggct ggttcccggt
    12901 gcagggttgg ggcagcacgt tcaccgacag cctgctcgtc tacgccgcgc tgccgatcct
    12961 ggtcggggtg gtcgtctcgc tcgcgccgga catccgcctc taccgcagct tcttcctcga
    13021 ggagatgggg caggactacg tgcgtaccgc gcgggcgaaa ggcctcggcg aaggtcgcgt
    13081 gatgtggctc cacgtgctgc gcaacgccgc catcccgatc gtgaccaacg tcatgacgca
    13141 ggttccgggg ctgctcgccg gcgctttcct catcgagcga ttcttctcga ttccgggcat
    13201 cggccgcgag gtgatcctcg cggtggagcg cagcgacttt ccggtgatca aggcggtcac
    13261 ggtgtacgtc gcgatcgcca ccatggtcat caacctcgcg gcggatctgc tctaccgcac
    13321 gcttgatcca cgggtacagc tcaaatgagc ttcttccaat gagcctttgg cggctcgcct
    13381 ggcggcgctt tctgcgcgac cgcgtcggtc tggtgtctct cgtgatcgtc atcgctttcg
    13441 tcgcgctcgc gtgcgcctcg gccgcggggt gggtggcccg cgactgggcc gaggaggcgg
    13501 gcgtcagcta tgcgctgccc cggttcctgg gttcacaggt cgaggaagcc gccgcccagg
    13561 gggccgcaac ggtaggaccc caggaaaccc atgggatccc cgatccgctc gccgaagagt
    13621 tggcggaaat acaaaaggat ttgttgggct tgtcgggtac cgcgtcccgg gcgggaaccc
    13681 ttgtcttcgg cggcgacaag tggggccgcg acgtggtgaa gaaggcgatc aagggcaccg
    13741 agacttcgct ggtcgtgggg ctcgccgccg cggcgctcgc cgcattcctc ggcacgctct
    13801 ttggcgcggc tgcgggctac tacggccgct gggtggacga tttcttcaac tggctctaca
    13861 gcgtatttac ctcgatccct tatctgctgc tggtgctggc ggtcgccgcg gtgctgaacc
    13921 agaaggggac cgtcaccgtg atcctgatcc tcggcctcac cggctggacc ggaatcttcc
    13981 ggctgatccg cgccgagtac ctcaagcaca aggtgcgcga atacgtcctc gcggccgacg
    14041 ccatcggagc ctcggatgcg cggcgcatgt tcgcccacat cctgccgaac gtgagccacg
    14101 tcgtcctggt gcagctctcg ctgcacgtgg tgctgttcat caagtccgag gtgatcctga
    14161 gcttcctcgg cttcggcgtc ggggtggaca cggtgtcctg gggcagcatg ctggcggaag
    14221 cgccgagcga gctgatcctc ggcatgtggt ggcaggtcgc cgcggcgacg ctcgccatgg
    14281 cgctcgccgt taccgccttc agcctgttta ccgactccgt gcgcgacgcg ctcgatccga
    14341 ggctgcaatg agcgtgctct cggtgcggga cctgcgagtc gatttcggca cgcttcaggc
    14401 tgtgaagggc atcagcttcg agatcccgga gcaca
//
LOCUS       DATBSZ010000268         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_666404, whole genome shotgun sequence.
ACCESSION   DATBSZ010000268 DATBSZ010000000
VERSION     DATBSZ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666404"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VIG70_17400"
     CDS             <1..252
                     /locus_tag="VIG70_17400"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY8252370.1"
                     /translation="IVERVLKRKDVAFLGLIGSETKAAKFRLRLQQRGVSAEGLVCPV
                     GLFKAGKHPAEVAVSAVAQLLERRAQVPVGGALEGVARA"
     gene            complement(193..1572)
                     /locus_tag="VIG70_17405"
     CDS             complement(193..1572)
                     /locus_tag="VIG70_17405"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8252371.1"
                     /translation="MDRANRNDLTALSCVELAAAIRERRATCVAAMEAVLERARGVQP
                     RLNCFLRIDEDAALAAAHLADREVARGYVRGPLHGVPMAHKDMYYRRGVASSCGSKIL
                     RDVPATATAKVLEHLDAAGAIQFGVLNMAEFAMGPTGHNWHYGHCRNPWDPERITGGS
                     SSGSGASVAARAAFAALGSDTGGSVRLPAAFCGISGVRPTHGRVSVENILPLCPSLDT
                     VGPLTRTVEDAAVVLSVIAPGFACNLEKPVAGLRIGRPRQLFYDGCDAEIAQAMEASL
                     EVFRQLGAAVVDVDLPDFSKLSTLAATLHAAEANPFHDEWFRTRPQDYSAQVRERLEK
                     GRPVLALDYVKALQARGPAIAAFCESVFSKVDLVHGPVVSFQTPTVAETDLSTGEAAA
                     QMLARFVRLTRPISYLGLPVACANAGFTKAGMPIGMQLIAPPLDEATALRAGHAFQRA
                     TDWHLRAPL"
     gene            complement(1545..2225)
                     /locus_tag="VIG70_17410"
     CDS             complement(1545..2225)
                     /locus_tag="VIG70_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252372.1"
                     /translation="MKLDVQASASPLDLLQLDAAKSENRILEHPVWKAALDGTLPKPR
                     LKRLLVAFYPVVAGPGRYAFAAKVSQIDAQDGKELFLQLHESLKNPAADADAGWKKAL
                     LALGATQRELDAEPSAEAADLVDVLREHGLRSGPVHAAVIAYMLEQHLPRLWGALAEA
                     LQKHYGVKAGALEHLRFEAGRAAKVEKWVGHLVDKYVASADPYTVFEARRSAREAIWA
                     WTALTETI"
     gene            complement(2222..2440)
                     /locus_tag="VIG70_17415"
     CDS             complement(2222..2440)
                     /locus_tag="VIG70_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252373.1"
                     /translation="MKKAMPPKGMPWSAPVLIRKDGKKLAVEVPPQVAADLKAGPGDV
                     LNFTKLPDGEIQIWVVKKSGYASLKDMK"
     gene            complement(2437..3162)
                     /locus_tag="VIG70_17420"
     CDS             complement(2437..3162)
                     /locus_tag="VIG70_17420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8252374.1"
                     /translation="MCLHGITANAHVFEPMAELLAARFRITSIDQRGHGRAAKPAIGY
                     AAEDYARDINDRLSEKTLLIGHSLGARNALVAGASYPDKVAAVVAIDFTPYIEPEVFD
                     ALEARVEAGGRDFASLEEIRKYLRERYPRLPADAIERRARHGYESVDGKWRPLAAPAA
                     MRETCFGLRHDLSSTLKEIRVPVLLLRGEHSKLVSRHAWVKTCALRPDLPAQEIAGAD
                     HYVPEEQPAAVASAVLDFWDAIQ"
BASE COUNT          525 a         1131 c         1097 g          533 t
ORIGIN      
        1 atcgtcgagc gcgtcctcaa gcgcaaggac gtcgcgtttc tcggcctgat cggctcggaa
       61 acgaaggccg cgaaattccg ccttcgcctt caacaacgag gagtttctgc ggaaggcctg
      121 gtttgccccg tcggcctttt caaggccggc aagcatccgg ccgaggtcgc ggtgtcggcg
      181 gtcgcgcagc tgctagagcg gcgcgcgcag gtgccagtcg gtggcgcgct ggaaggcgtg
      241 gcccgcgcgt aaggcggtcg cttcgtcgag cggcggggcg atgagctgca tgccgatcgg
      301 catgcccgcc ttcgtgaacc ccgcattggc gcacgccacc ggcagcccga ggtagctgat
      361 cggccgggtg aggcgcacga agcgcgcgag catctgcgcc gcagcctcgc cggtggagag
      421 gtcggtctcg gcgaccgtcg gcgtctggaa cgacaccacc ggcccatgca ccaggtccac
      481 cttcgagaac accgactcgc agaacgcggc gatcgcgggg ccgcgggcct gcagcgcctt
      541 cacgtagtcg agcgcgagca ccggccggcc cttttccagc cgctcgcgca cctgggccga
      601 gtaatcctgc ggccgggtcc ggaaccattc gtcgtggaac gggttcgcct cggcggcgtg
      661 aagcgtcgcg gcgagcgtgc tcagcttgct gaaatcgggc aaatccacgt ccacgacggc
      721 ggcaccgagc tggcgaaaaa cctcgagcga cgcctccatc gcctgagcga tctcggcatc
      781 gcagccgtcg tagaagagct gccgcggccg gccgatgcgc agccctgcaa cgggcttttc
      841 gaggttgcat gcaaacccgg gggcgatgac gctgaggaca actgccgcgt cctcaacggt
      901 gcgcgtgagc ggacccaccg tgtcgagcga cgggcagagc ggaaggatgt tctcgaccga
      961 cacccggccg tgcgtcggcc gcacacccga aatgccgcag aacgccgctg gcagccgcac
     1021 cgagccgccg gtgtccgagc caagcgcggc gaaggcggct cgcgcagcca ccgacgcacc
     1081 cgagcccgac gacgagccgc cggtgatgcg ctcgggatcc cagggattgc ggcaatggcc
     1141 gtagtgccag ttgtgccccg tggggcccat ggcgaattcc gccatgttga gcacgccgaa
     1201 ctggatcgcg cccgcggcgt cgaggtgctc gaggaccttc gccgtcgccg tggccgggac
     1261 gtcgcgcagg atcttcgagc cgcagctcga ggccacgccg cggcggtagt acatgtcctt
     1321 gtgcgccatc ggcacgccgt gcagcggccc gcgcacgtag ccgcgcgcca cctcgcggtc
     1381 ggcaagatgc gccgccgcca gcgccgcgtc ctcgtcgatg cgcaggaagc agttgagccg
     1441 aggttgtaca ccgcgcgctc gctcgagcac cgcttccatc gccgcaacgc aggtcgcgcg
     1501 ccgctcgcgg atcgccgcgg cgagctcgac acaagagagc gcggtcagat cgtttcggtt
     1561 agcgcggtcc atgcccagat cgcctcacgc gccgagcggc gcgcctcgaa caccgtgtag
     1621 ggatcagcgc tcgcgacgta cttgtcgacg aggtgcccaa cccatttctc caccttggcg
     1681 gcgcggccgg cctcgaaacg caggtgctcg agcgcgccgg ctttcacgcc gtaatgcttc
     1741 tgcagggctt cggcgagcgc gccccacagc cgcggcaggt gctgctcgag catgtaggcg
     1801 atcaccgccg catgcaccgg gccggagcgc aggccgtgct cgcgcagcac gtcgacgaga
     1861 tccgccgcct cggctgacgg ttctgcatcc aattcacgtt gcgtcgcccc gagagcgagc
     1921 aaagcctttt tccatcccgc atcggcgtcg gccgcggggt tcttcagcga ttcgtggagc
     1981 tggagaaaaa gctcttttcc gtcctgcgca tcgatctgcg acaccttggc ggcgaacgcg
     2041 tagcggcccg gcccggcgac gaccggatag aacgcgacga gcagtctctt cagccgcggt
     2101 ttgggcagcg ttccgtcgag cgccgctttc cacacgggat gttcaagaat ccggttctcg
     2161 gacttcgccg cgtccaactg cagaagatcg agcggcgaag cgctcgcctg gacgtcgagc
     2221 ttcatttcat gtccttcagc gatgcatagc cgctcttctt caccacccag atctggatct
     2281 cgccgtccgg caatttggtg aagttcagga catcccccgg acccgccttg agatcggcgg
     2341 ccacctgcgg cggaacctcg accgcgagct tcttgccgtc cttgcggatc agcacggggg
     2401 cggaccaggg cattcccttc ggcggcatgg cttttttcat tggatggcgt cccagaaatc
     2461 gagcactgcg gaagcgacgg cggccggctg ctcttccggc acgtagtgat cggcgccggc
     2521 gatctcctgc gccgggaggt ccggccgcag ggcgcacgtc ttgacccatg cgtggcgcga
     2581 cacgagcttg ctgtgctcgc cgcgcaacag caggaccggc acgcggatct ctttcagcgt
     2641 ggaagagaga tcatggcgta gaccgaagca tgtttcgcgc atggctgcag gcgccgccag
     2701 cggccgccac ttgccatcga cgctctcgta cccgtggcgc gcgcggcgct cgatcgcatc
     2761 ggccggcagg cgcggatatc gctctctcag gtatttccgg atctcctcca gcgaggcgaa
     2821 gtcgcgtcct cccgcctcga cccttgcctc gagagcgtcg aatacctccg gctcgatgta
     2881 gggcgtgaag tcgatcgcca cgactgcggc aaccttgtcc ggatagctcg cgccagccac
     2941 gagggcgttt cgcgcgccga gagagtggcc gatcaacaag gttttttcag aaagtctgtc
     3001 attgatatcg cgagcatagt cctcggccgc atagccaatc gccggcttcg cggcgcgccc
     3061 gtgcccgcgc tggtcgatgc tggtgatgcg aaagcgcgcg gcgaggagct cggccatcgg
     3121 ctcgaacacg tgcgcgttcg ccgtgatgcc atgcaggcac agggccgcgg gcctagaagt
     3181 cgtacttctg tctcaaatag cgaaggatgc cgtcgtgcat ctcgcgccac gcccacagcg
     3241 tgactgcggc cgcgcgccgg ccgcgcacga cgtcgccgcg gtcctt
//
LOCUS       DATBSZ010000269         7468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_463428, whole genome shotgun sequence.
ACCESSION   DATBSZ010000269 DATBSZ010000000
VERSION     DATBSZ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463428"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VIG70_17425"
     CDS             <1..507
                     /locus_tag="VIG70_17425"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY8252375.1"
                     /translation="RCLSEAPHYDATVAVLSYEFPADALVHALKFRGELALAGLLGGM
                     LAQRISSHEIDCVIPVPLSAERLRTRGYNHAAEIARHLRTAKLDLALCVRSRDTPPQM
                     DLPYDERRRNVRGAFRCTRALAGESVAVVDDVMTTGATLDEIASVLKTAGATRVVNWV
                     VARTFPRD"
     gene            507..971
                     /locus_tag="VIG70_17430"
     CDS             507..971
                     /locus_tag="VIG70_17430"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY8252376.1"
                     /translation="MFHIVLVHPEIPPNTGNVIRLAANTGSRLHLVEPLGFTMDDRQL
                     RRAGLDYHELSHIEVHKEWDACKSALAGRRMFALSTHGTRNFAEVEFRVGDAFVFGPE
                     TKGLGQERLEEFPAAMRLRLPMLPGNRSLNLSNAVAVVVFEAWRQIGFLGSG"
     gene            complement(968..1945)
                     /locus_tag="VIG70_17435"
     CDS             complement(968..1945)
                     /locus_tag="VIG70_17435"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY8252377.1"
                     /translation="MKRVGVLGAGAWGTSMACVLAARLDVTLWARDPAQAEALAHTRR
                     NERYLRGIEIPAAVKVTSNLDDASRAELVLAATPVSGLRDVLRKIQNCNALVWLCKGF
                     EQGTGLLPHQIVERTLGPGARYGALSGPSFAEEVARGLPCALTLAALDAGFAREAAAL
                     VHGGRMRVYYSADLVGVEIGGAVKNVMAIAAGISDGLGLGHNARAALITRGLAEIARL
                     GAAMGGQSETIFGLTGAGDLILTATGDLSRNRRVGLQLAQGLSLEKILHDLGHVAEGV
                     YTAREIAKLAKARGVEMPVTDAVNAVLEGKLAPKAAVEQLLARDPKKER"
     gene            complement(1942..2430)
                     /gene="secB"
                     /locus_tag="VIG70_17440"
     CDS             complement(1942..2430)
                     /gene="secB"
                     /locus_tag="VIG70_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY8252378.1"
                     /translation="MSEPQTQATFQIEKIYVKDVSLEIPNAPQIFMQQAQPQLEVRID
                     TGAAPFSEGYFEASVSATVTAKVGDKTLFLAEAVQAGIFQMRNVPPDDLKPLLGIACP
                     TILFPYLRETISDLVIRGGFPPVLLAPVSFEALYVQRLQQEQSAAPGGASPEGPRIEV
                     AR"
     gene            complement(2441..2698)
                     /gene="grxC"
                     /locus_tag="VIG70_17445"
     CDS             complement(2441..2698)
                     /gene="grxC"
                     /locus_tag="VIG70_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004200200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY8252379.1"
                     /translation="MSRVLMYATAACPFCQSAERLLVAKGAQIEKIRVDLEPERRAEM
                     TKKSGRHTVPQIWIGERHIGGCDDLYALDREGRLDPLLKAK"
     gene            complement(2695..3099)
                     /locus_tag="VIG70_17450"
     CDS             complement(2695..3099)
                     /locus_tag="VIG70_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8252380.1"
                     /translation="MDFVRNNLLLFAVALVSGAMLLWPLFRRTTGGPWVNTAEATHLI
                     NREDALVVDVRDAGEYGAGHILGAKNVPVSRLDEGDLAKRKERPVIVYCDGSERSSKA
                     VSALKKQGFTRVVNLSGGLKAWQQAGLPIEKQ"
     gene            3126..3866
                     /gene="gpmA"
                     /locus_tag="VIG70_17455"
     CDS             3126..3866
                     /gene="gpmA"
                     /locus_tag="VIG70_17455"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY8252381.1"
                     /translation="MRKLVLLRHGESDWNRENRFTGWTDVDLSEKGTGEARAAGKLLK
                     AEGYAFDLAHTSVLRRAIRTLWLALDELGQMWLPVEKSWRLNERHYGALQGLNKAEMA
                     AKFGEQQVLVWRRSYDTPPPELDRADPRYEGNDARYAGVKVPLTECLKDTVARVVPYW
                     QSSIAPAVRAGKRVLIAAHGNSLRALVKHLDGISDEKIVEKNIPTGIPLVYELDHALK
                     PARSYYLGDADEVARRVASVAAQGKAKV"
     gene            3881..4975
                     /locus_tag="VIG70_17460"
     CDS             3881..4975
                     /locus_tag="VIG70_17460"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8252382.1"
                     /translation="MVFGLVAGAAWGASQEENLKALRGRIEALNRDLEKKEGSQREAR
                     DALRDSERAISNANRALAQLAAEARQVRAEAARVAERRRSVQAQVSHREAAIERMLVA
                     WAAGGAPDALRVALSGSDPADIGRRLHYFGHLSRAAASLLAEQRAALEELERLANEAR
                     GRAERLRAIEQASRADRDRVIRERRERSRVLQSLAVDIRKNRKEIRVLRADEARLARL
                     VEAVGRGDFASFKDPFSALRGKLRLPVRGELTRRFGAPRGAAGTEAKGVFIRAPGGQP
                     VRAIAKGQVVYADWMRGFGNLLIVDHGENYLSIYANNESLLKELGDAVAPGEPIATTG
                     SSGGNEETGLYFEMRHLGRAFDPLSWVKPK"
     gene            4986..6410
                     /locus_tag="VIG70_17465"
     CDS             4986..6410
                     /locus_tag="VIG70_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY8252383.1"
                     /translation="MHTRLRNIGFMVVGAVAGVLISLNFQAIADRATRTSLPIEELRA
                     FTEVFGAIKTNYVEPVEDKKLITEAINGMLTGLDPHSSYLDAEAFRELQVGTQGQFGG
                     LGIEVGMEDGFVKVISPIEDTPAHKAGIKPGDLIIKLDDTPVKGMTLNDAVKKMRGKQ
                     GTQITLTITRKGETAPLTVTITRAIIRVQSVKSKMIEPGFAWIRVSQFQEATAEHLVK
                     HLEGLFKQGQVKGLVLDLRNDPGGLLHGAVAISSAFLPAKSLVVSTDGRAEDARKKFY
                     ASPEDYARRGDDVLRNLPAAVKSVPMVVLVNGGSASASEIVAGALQDHKRAKVMGTQT
                     FGKGSVQTIMPLGNNTAIKLTTARYYTPGGRSIQALGVTPDIMVEDPSDTLVRVREAD
                     LQRHLENGRQPEKKDSKDAAQPRVKPAPGAATSTDAKPVELGSKEDFQLSQALAFLKG
                     EPVKNQSPTVAQTDAPKQQVKPTN"
     gene            6407..7150
                     /gene="moeB"
                     /locus_tag="VIG70_17470"
     CDS             6407..7150
                     /gene="moeB"
                     /locus_tag="VIG70_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY8252384.1"
                     /translation="MNDQQLLRYSRHILLPEIGIEGQEKLRAARALIVGAGGLGCPAA
                     LYLAASGVGRLTLADADKVDLTNLQRQILYRTESVGAVKVEAARSALNAINPDVAVVS
                     LQKRVVDFSELVAQADVVLDCTDNFATRHAINRACVQHRKPLVSGAAIRFDAQLMVFD
                     LRSRESACYACLFPEDAAVEEVQCSTMGVFAPLTGVIGAMQSMEALKLVAGVGVSLNG
                     KLQVFDAKSAEWRTVRVKKDPGCSVCSKR"
BASE COUNT         1280 a         2533 c         2504 g         1151 t
ORIGIN      
        1 cgctgcctga gcgaggcgcc gcactacgat gccacggtgg ccgtgctgtc ctacgaattc
       61 cccgccgatg cgctggtcca cgcgctcaag ttccgcggcg agctcgcgct cgcagggctg
      121 ctgggaggaa tgcttgccca aagaatctcg agccatgaga tcgactgcgt gatccccgtc
      181 cccctctccg ccgagcgcct gcgcacccgc ggctacaacc acgccgcgga gatcgcgcgc
      241 cacctgcgca cggcgaagct cgacctcgcg ctctgcgtgc gcagccgcga tacgccgccg
      301 cagatggacc tgccgtacga cgagcgcagg cgcaacgtgc gcggcgcgtt ccgctgcacc
      361 cgcgcgctcg ccggcgagag cgtggccgtg gtcgacgacg tgatgaccac cggtgcgacc
      421 ctggacgaga ttgcttcagt cctgaaaacc gctggcgcaa cccgcgtggt caactgggtg
      481 gtggcccgca ccttccctcg agactgatgt tccacatcgt cctcgtccat cccgagatcc
      541 cgccgaacac cggcaacgtg atccgcctcg ccgcgaacac cggctcgcgc ctgcacctcg
      601 tcgagccgct cggcttcacc atggacgacc gccagctgcg gcgcgccggg ctggattatc
      661 acgagctgag ccacatcgaa gtgcacaagg aatgggacgc ctgcaagagc gcgctcgccg
      721 ggcggcgcat gttcgcgctg agcacgcacg gcacgcgcaa tttcgccgag gtcgagttcc
      781 gcgtcggcga cgccttcgtg ttcggccccg agaccaaagg cctcggccag gagcggctcg
      841 aggaatttcc cgccgccatg cggctgcgcc tgccgatgct ccccggcaac cgcagcctca
      901 acctgtcgaa cgccgtggcg gtcgtggtgt tcgaggcctg gcgccagatc ggctttctcg
      961 gctcgggcta gcgctccttt tttggatctc tcgcgagaag ctgctcgacc gcagccttcg
     1021 gcgcgagctt tccctccagc accgcattca ccgcatcggt gaccggcatc tcgacgccgc
     1081 gcgccttggc gagcttcgcg atctcgcgcg cggtgtacac gccttccgcg acgtggccga
     1141 ggtcgtgcag aattttttca agagagaggc cctgggcgag ctgcaatccc acgcgccggt
     1201 tgcgcgaaag gtcgccggtc gcggtgagga tgaggtcgcc ggccccggtc agcccgaaga
     1261 tcgtttcgga ctggccgccc atcgcggcgc ccaggcgcgc gatttccgcc aggccgcgcg
     1321 tgatgagcgc ggcgcgcgcg ttgtgcccga gcccgagccc gtcggagatg cccgcggcga
     1381 tcgccatcac gttcttcact gcgccaccga tctccactcc gaccagatct gcgctgtagt
     1441 acacgcgcat cctgcccccg tgcaccagcg ccgcggcctc gcgcgcgaat ccagcgtcga
     1501 gcgcggcgag cgtaagcgcg cacggcagcc cgcgcgccac ttcctcggcg aacgacggcc
     1561 ccgacagcgc gccgtagcgc gcgccgggac cgagcgtccg ctcgacgatc tggtgaggaa
     1621 gaaggccggt gccctgctcg aagcccttgc acagccagac cagcgcgttg cagttttgga
     1681 tttttcgaag gacatccctc aaccccgaga cgggagtggc ggcgagcacg agttcggcgc
     1741 gcgatgcgtc atccaagttg gaagtgacct tcaccgcggc cggaatctcg atcccacgca
     1801 gatagcgctc gttcctgcgc gtgtgcgcga gcgcctcggc ctgcgccgga tcgcgcgccc
     1861 acagcgtcac gtccaggcgc gccgccagca cgcacgccat cgaggtgccc caggcgcccg
     1921 cgccgagcac cccgacgcgc ttcaccgcgc gacttcgatc ctggggcctt ccggcgaagc
     1981 gcctcccggg gctgcggact gctcctgctg cagccgctgc acgtagaggg cttcgaacga
     2041 gaccggcgcg agcaggaccg gcgggaatcc gccgcggatc acgagatcgg agatcgtctc
     2101 gcgcaggtag ggaaagagga tcgtcgggca ggcgatgccg agcagcggct tgagatcgtc
     2161 cggcggcacg ttgcgcatct ggaagatgcc cgcctgcact gcttcggcga ggaacagcgt
     2221 cttgtcgccg accttggcgg tgaccgtcgc gctgaccgaa gcctcgaagt agccctcgga
     2281 gaacggcgcc gcgccggtgt cgatgcgcac ctcgagctgc ggctgcgcct gctgcatgaa
     2341 gatctgcggc gcgttcggga tctcgagcga aacgtccttc acgtagatct tctcgatctg
     2401 gaacgtcgcc tgcgtttggg gttcgctcat gggtcgtttc tcatttggct ttaagcagcg
     2461 ggtcgagtcg tccctcgcga tcgagggcgt agaggtcgtc gcagccgccg atgtggcgct
     2521 cgccgatcca gatctgcggc accgtgtgcc ggccgctctt cttcgtcatc tcggcgcggc
     2581 gctcgggctc gaggtcgacg cggatcttct cgatctgcgc gcccttggcg acgagcaggc
     2641 gctcggccga ttggcagaac gggcacgccg cggtggcata catcagaacc cgactcattg
     2701 cttttcaatg ggcaggccgg cttgctgcca agccttgaga ccgcccgaaa ggttcaccac
     2761 ccgcgtgaag ccctgctttt tcagggccga gacggctttc gaggagcgct cgctgccgtc
     2821 gcagtagacg atcaccggac gctccttgcg cttcgcgagg tcgccttcgt cgaggcgcga
     2881 caccggcacg tttttcgccc ccaggatgtg gccggcgccg tattcgcccg cgtcgcgcac
     2941 gtcgacgacc agcgcatcct cgcggttgat gagatgcgtt gcttcggcgg tgttcaccca
     3001 cgggccgccg gtcgtgcgcc ggaaaagcgg ccagagcagc atcgcgcccg agacgagggc
     3061 gacggcgaag aggaggaggt tattgcggac gaaatccacc gagccggtca ttataatgaa
     3121 ggccgatgcg caagctcgtg cttctgcgcc acggcgagtc ggactggaac cgcgaaaatc
     3181 gcttcaccgg ctggaccgac gtcgacctct ccgagaaggg caccggcgaa gcccgcgccg
     3241 ccgggaagct cctcaaggcc gagggctacg ccttcgatct cgcccatacc tcggtcctgc
     3301 ggcgcgccat ccgcacgctg tggctcgcgc tcgacgagct cggccagatg tggctgccgg
     3361 tggagaagag ctggcggctg aacgagcgcc actacggcgc gctgcagggg ctcaataagg
     3421 ccgagatggc ggcgaagttc ggcgagcagc aggtcctggt ctggcggcgc agctacgaca
     3481 cgccgccacc ggagctcgat cgcgcagatc cgcggtacga gggtaacgac gcccggtacg
     3541 ccggagtcaa ggttcccctg accgagtgcc tcaaggacac cgtcgcgcgg gtggtgccgt
     3601 actggcagtc gagcatcgcg cccgcggtgc gcgccggcaa gcgggtgctg atcgcggcgc
     3661 acggcaactc gctgcgcgcg ctggtgaagc atctcgacgg catctcggac gaaaagatcg
     3721 tcgagaaaaa catcccgacc ggcattccgc tcgtgtacga gctcgaccat gcgctcaagc
     3781 ctgcccggag ctactacctc ggggatgcgg acgaagtcgc gcggcgcgtc gcctcggtcg
     3841 ccgctcaagg gaaagcgaag gtctgaaggg cgtcgtcttc gtggttttcg gtttggtcgc
     3901 cggcgcggcc tggggtgcaa gtcaagaaga aaatctgaag gcgctgcgcg gccgcatcga
     3961 ggcgctcaac cgggacctgg agaaaaaaga aggttcgcag cgcgaggcgc gcgacgcgct
     4021 gcgcgattcc gagcgcgcga tctccaacgc gaaccgcgcg ctcgcgcagc tcgccgcgga
     4081 ggcgcgccag gtgcgcgccg aggccgcgcg cgtcgccgag cggcgccgct cggtgcaagc
     4141 gcaggtgtcc caccgggaag cggcgatcga gcggatgctc gtcgcctggg cggccggcgg
     4201 cgcccctgac gcgctgcgcg tcgcgctttc cggcagcgat cccgcggaca tcggccggcg
     4261 cctgcattac ttcggccatc tctcgcgcgc ggcggcgagc ctgctcgccg agcagcgcgc
     4321 cgccctcgag gagctcgagc gcctggcaaa cgaggcaaga ggccgcgccg agcgcctgcg
     4381 cgcgatcgag caggcgagcc gcgccgatcg cgaccgggtg atcagggagc ggcgcgagcg
     4441 cagccgggtg ctgcagtcgc tggcggtcga tatccgcaag aaccgtaagg aaatccgcgt
     4501 gctgcgggcc gacgaggcgc gcctcgcgcg gctcgtcgag gcggtcggcc gcggcgattt
     4561 tgcatcattt aaagatccgt tctccgcgct tcgcgggaaa cttcgcctgc cggtgcgggg
     4621 ggaactgacg cggcgcttcg gcgctccaag gggagcggcg ggaacggagg caaaaggcgt
     4681 gtttatccgc gctcccgggg ggcagccggt gcgcgcgatc gccaagggcc aggtggtcta
     4741 tgccgactgg atgcgcgggt tcggcaacct gctgatcgtg gaccatggtg agaactatct
     4801 ctcgatttac gccaacaacg agtcgctgct gaaggaattg ggcgacgcgg tggcgccggg
     4861 agagccgatc gccaccaccg gttcgagcgg tggcaacgag gaaacgggtt tatactttga
     4921 aatgcggcac ttgggccgcg cctttgaccc gctgtcgtgg gtcaagccga aataaagcga
     4981 gcatcatgca caccaggctt cgcaacatcg gattcatggt cgtcggcgcc gtcgccgggg
     5041 ttctgatcag cctgaacttc caggcgatcg ccgaccgcgc cacgcgcact tcgctaccga
     5101 tcgaggagct gcgcgcgttc accgaggtct tcggcgcgat caagaccaac tacgtcgagc
     5161 cggtggagga caagaagctc atcaccgagg cgatcaacgg catgctgacc ggcctcgacc
     5221 cgcattcgtc gtatctcgac gcggaagcgt ttcgcgagct gcaggtcggc acccagggcc
     5281 agttcggcgg cctgggcatc gaggtgggca tggaagacgg cttcgtcaag gtgatctcgc
     5341 cgatcgagga caccccggca cacaaggccg gcatcaagcc cggcgacctg atcatcaagc
     5401 tcgacgacac gccggtgaag ggcatgacgc tcaacgacgc cgtgaagaag atgcgcggca
     5461 agcagggcac ccagatcacg ctcaccatca cgcgcaaggg cgagaccgcg ccgctgaccg
     5521 tcaccatcac gcgcgccatc atccgcgtgc agagcgtgaa gtcgaagatg atcgagcccg
     5581 gcttcgcctg gatccgcgtg tcgcagttcc aggaagcgac cgccgagcac ctggtgaagc
     5641 acctcgaagg cctgttcaag cagggacagg tgaagggcct ggtcctcgac ctgcgcaacg
     5701 atcccggcgg cctgctgcac ggcgcggtcg cgatctcgtc cgcgttcctg ccggcgaagt
     5761 cgctggtcgt gtcgaccgac ggccgcgcgg aggacgcgcg caagaagttc tacgccagcc
     5821 cagaggacta tgcgcggcgc ggcgacgacg tcttgaggaa cctgccggcc gcggtcaaga
     5881 gcgtgccgat ggtggtgctg gtgaacggcg gctccgcctc ggcttccgag atcgtcgccg
     5941 gcgcgctgca ggaccacaag cgcgccaagg tgatgggcac gcaaaccttc ggcaagggct
     6001 cggtgcagac catcatgccg ctcggtaaca acaccgcgat caagctcacc accgcgcgct
     6061 actacacgcc cggcgggcgc tcgatccagg cgctcggcgt cacgcccgac atcatggtcg
     6121 aagacccgag cgacaccctg gtgcgcgtgc gcgaagccga cctgcagcgc cacctcgaga
     6181 acggccgcca gccggagaag aaggacagca aagatgccgc gcagccgcgc gtcaagccgg
     6241 cgccgggcgc tgccacctcg accgacgcca agccggtcga gctcggctcg aaggaagact
     6301 tccagctttc ccaggcgctc gccttcctca aaggcgagcc ggtgaagaac cagtcaccca
     6361 cggtcgccca gaccgacgcg ccgaagcagc aagtcaaacc cactaactga acgatcagca
     6421 gcttctccgg tacagccggc acatcctgct ccccgagatc gggatcgagg ggcaggagaa
     6481 gctgcgcgcc gcgcgcgcgc tgatcgtcgg cgcaggcggc ctcggatgcc cggcggcgct
     6541 ctacctcgcc gccagcggcg tcggccgcct cacgctcgcc gatgcagaca aggtcgacct
     6601 caccaacctg cagcggcaga tcctctaccg cacggaatcg gtcggcgcgg tcaaagtcga
     6661 ggcggcgcgc tcagcgctta acgcgatcaa tcccgatgtc gccgtcgtct cattgcagaa
     6721 gagagtcgtg gatttctccg agctcgtcgc ccaggccgac gtggtcctcg actgcaccga
     6781 caacttcgcc acgcgccatg cgatcaaccg agcgtgcgtg cagcaccgca agccgctggt
     6841 ttccggagca gccatccgct tcgacgcgca gctcatggtg ttcgacctga ggagccggga
     6901 atcggcttgc tacgcttgcc tgttccccga ggacgccgcg gtcgaggaag tgcagtgctc
     6961 gacgatgggc gtgttcgcgc cgctcaccgg cgtgatcggg gccatgcagt cgatggaggc
     7021 gctcaagctc gtcgcgggtg tcggcgtgtc gctgaacgga aagctccagg tgttcgatgc
     7081 gaagagcgcc gagtggcgca ccgtgcgggt gaagaaagac cccggatgca gcgtctgcag
     7141 caagagataa tctagcgaac tcccagccca gcgggcatcc cacaaaaacg tcataaaacg
     7201 ctcaatttcg ggctgatggg cgataccccc tactaaggat aggaaaattc cttcgcgtcc
     7261 gtgccgcgaa atgcccgcgg ttgcggcgtg tttcgggtcg gtgagcggtg cttctcgccc
     7321 tgatcgatct ggccgcggtg ggcggattga atttcctatg tagaggcagc gcccgcgcga
     7381 ccgtccttca cttcagcaga cgataatcac tcaagcggaa ccacttgcgg gaaaggatga
     7441 acccggtcac cggttccggg gagaggct
//
LOCUS       DATBSZ010000270        12226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_599086, whole genome shotgun sequence.
ACCESSION   DATBSZ010000270 DATBSZ010000000
VERSION     DATBSZ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599086"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..308)
                     /locus_tag="VIG70_17475"
     CDS             complement(102..308)
                     /locus_tag="VIG70_17475"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEY8252385.1"
                     /translation="METVELKVEGMDCEGCVKSVTRMLSGLAGVAKADVSLAQASAKV
                     TYDPAKVDVGQMKKAVERAGYKAP"
     gene            complement(290..2356)
                     /locus_tag="VIG70_17480"
     CDS             complement(290..2356)
                     /locus_tag="VIG70_17480"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY8252386.1"
                     /translation="MNVVQLDIEGMTCAACAARIEKNLNRLEGVEASVSLATDKATVR
                     FDSKLEVESIIQRIRDTGYDARVAREGELRDHSAEHRKARNEFVLAAVLTAPLLLHGF
                     VPGWAQCILATPVQLWSGRRFYRGAWNALRGGTANMDVLVGLGTSAAYGFSAVVLFFQ
                     LNEQLYFEAGAVVIALVLLGKYLEARAKAKAVQALDSLIRLQPKTTFVERNNALVEVD
                     IAALNAGDAFVVRPGDAVALDARVVDGSSAVDESMLTGESVPKEKRIGDAVFAGTING
                     EGLLKCVATATGKATMLAGIIRLVAAAQASKPPVQRLVDRVSAVFVPVVMGVSVLTLL
                     FWWWLGGFAIAILPAVAVLVIACPCALGLATPTALIVGVGRAARAGILIRNAAALEGA
                     GALDTLVLDKTGTLTRGEPAVTDVFSSNPDELLRVAAALESRSGHPLARAIVVRFGGA
                     PQPVAEFRAHGGRGVSGVVGGKPARLGSPAFLGREKSVEGKSVVGVEWDGRFLGWIAL
                     GDELRATSAQAVARLARMGIEPVIVSGDSAEAVKAVAERLGIARWKAGVLPEEKLLEI
                     EKLKRENRRVGMAGDGVNDAPALAAADVSFALAAGSGAALEAADVTLMKNDLAGIADA
                     IRLSRATLAKIRQNLFFAFIYNVLGIPLAAAGMLNPMIAGAAMALSSVSVVSNSLWLN
                     RWKPSS"
     gene            complement(2370..2954)
                     /locus_tag="VIG70_17485"
     CDS             complement(2370..2954)
                     /locus_tag="VIG70_17485"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY8252387.1"
                     /translation="MSWFRRTPIAAARWVAIDCETSGLDAARDRLLSVGAVAVRDARI
                     DLSDCFEARVKQRAPSTADNILIHGIGGDAQLAGLPLLQVIQKLVAHVDDAIPVGFHA
                     DFDATVLRRHGFARRRWLDLAALAPALFPGRKAKALDDWLAEFGIAVHERHDALGDAF
                     STAQLLLVALAEAKRQGVDTVEGLRAAQRAGRWL"
     gene            complement(2951..4702)
                     /locus_tag="VIG70_17490"
     CDS             complement(2951..4702)
                     /locus_tag="VIG70_17490"
                     /inference="COORDINATES: protein motif:HMM:NF015410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEY8252388.1"
                     /translation="MADLIAPLRAHPPFDEMEPAALQFLAGHLAVGYYPRGELVVGPE
                     SGAVARLYIVKQGSVRGSGGAADVLLGPGECFPLGALAGRRATVYSYRAEKDSFCWEL
                     PAERFHELVERSARFRAFSTQHLAQLAERSQRALRAEAAESALDGAGMLAPLRSVLRR
                     EPVSCPRDTTLGEAIRRMHAEHVGSIAIVDDHAVPVGIFTTVDLLASAAAGTPLDAGI
                     AQHMTPRPFALEEEATLADAALAMARHGFRHIVVTRDGKLAGVVSERDLFALQRLGLR
                     RTAERIRGAGRLEPLVEAARDIRSLARQLLAQGVAAGPLTEMISALNDSLTRRVIELA
                     QARHPLAPAWCWLALGSEGRMEQTLVTDQDNALIMEGEKEAFLAFADQVNRDLDACGF
                     PLCKGDIMARNPRWCLTLQEWREKFDDWIRNHDPQALLNASVFFDFRALAGDARLAGA
                     LRSGILAQTRAHRAFCRALAETALQTRPPLGLLADFSADELDLKALGARPFVDAARVL
                     ALAAGSPETGTAARLRAAGEKNAIEAFHFVQTLRLRHARNLILRAELNDIDRRVLKEA
                     FRQAAMLQQRLRLDFAL"
     gene            complement(4717..5223)
                     /locus_tag="VIG70_17495"
     CDS             complement(4717..5223)
                     /locus_tag="VIG70_17495"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8252389.1"
                     /translation="MDEPLDTLQACHQGIEFELGVLENFAAHLAARGSDEAARSAASA
                     VMRYFDTVAAAHHRDEDEDLFPLLREAAARDGRDEICATLYELQREHQSMDRLYAQVR
                     AELQAIAVGESARLDAELVARFAWIHRRHMRMEGDLLLPYARQAIGPAQRAALGERMA
                     ARRQAAQA"
     gene            complement(5226..5573)
                     /locus_tag="VIG70_17500"
     CDS             complement(5226..5573)
                     /locus_tag="VIG70_17500"
                     /inference="COORDINATES: protein motif:HMM:NF020434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HEY8252390.1"
                     /translation="MSARSPSAVVLRPFPVLVACQGCAEFGQAARDVALRLDRLGFGE
                     PAWLGGAAEPAAARAKAKSRFPVFCLDACEKACARAWLAREGVAPQGCFVLSQAERSD
                     LEGAARRIAAAAA"
     gene            complement(5570..6016)
                     /locus_tag="VIG70_17505"
     CDS             complement(5570..6016)
                     /locus_tag="VIG70_17505"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8252391.1"
                     /translation="MYKHILIPTDGSETAEKAVEAGIAFARDANARVTWFTAVPEYEV
                     PGEGELLSKRHDISLEAHNNRSRALAEKILAEPARKARAAGITCAIDYAQSNQPYEAI
                     IAAATRHGCDLIFMSSHGRKVLPALFLGSATRDVLSHSTIPTLVYR"
     gene            complement(6009..6542)
                     /locus_tag="VIG70_17510"
     CDS             complement(6009..6542)
                     /locus_tag="VIG70_17510"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEY8252392.1"
                     /translation="MTPIRTAPELYAHAIAIEREAAERYAEFAERMNDLGNVALAELF
                     GRLAEIEAEHLDSLLRRTRGVALPHPSTTAYQWLDAAAPETAARELVFRLMTPRHALE
                     IALAGEKRAQAFFERVLITADDPALRALAREMAAEEQEHVRLVERLLERTPDPNVDWE
                     EVFEGKRAAQQKGAIDV"
     gene            6624..6854
                     /locus_tag="VIG70_17515"
     CDS             6624..6854
                     /locus_tag="VIG70_17515"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEY8252393.1"
                     /translation="MHYHYLTIEQREYLEKLIRSRVRGASQLETALEKLRQPDYGTCI
                     ECGKDVDYARLQIEPFALHCLACSRLPIPAGT"
     gene            6857..7096
                     /locus_tag="VIG70_17520"
     CDS             6857..7096
                     /locus_tag="VIG70_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252394.1"
                     /translation="MDRAMPYPLDYQLSLERTLRIRLDAVRAAGRAFETCILEETLAR
                     LRSAEFGTCLRCGGVIPFLEMAADPTRQTCRSCTR"
     gene            complement(7133..7720)
                     /locus_tag="VIG70_17525"
     CDS             complement(7133..7720)
                     /locus_tag="VIG70_17525"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8252395.1"
                     /translation="MKDAITIMKSEHRSISAVLLGLKELARMAQDARVRPRFEVLRSM
                     LRYIDEYPEKLHHPKEDEHLFARLVARAPETRPLVNELQAEHEEGARLIRELERALVF
                     LEEGWPAGAREFLAAVDAYAEFHWKHMRKEESELLPLAQRHLTAVDWKAIDAAFAANA
                     DPIAEVQERNFEKLFSRIANLAPAPVGLGEPWKRV"
     gene            complement(7736..9127)
                     /gene="hemN"
                     /locus_tag="VIG70_17530"
     CDS             complement(7736..9127)
                     /gene="hemN"
                     /locus_tag="VIG70_17530"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEY8252396.1"
                     /translation="MHYPASELVIDPVLIRKYDVSGPRYTSYPTADRFVEAFGEPQLR
                     QWLAKRNIGGFSQPLSLYVHLPFCENVCYYCACNKVVTRDHGRSAKYIKYLEKELALL
                     APLLGESCPIQAMHWGGGTPTFLTRDESSALVRAIESRFARNPKAEYSIEVDPRSAEP
                     GRMAFLAGLGFNRVSLGVQDFDAAVQKAVHRLQSVETTRRVMAEARASGFRSVNLDLI
                     YGLPKQTLDSFNRTLDEVLLLDPERIALYNYAHLPKLFKPQRRIAESELPSAERKLQL
                     MTLAIGRLTRAGYLYIGMDHFARPDDELAAAQTLGQLARSFQGYSTQAGDLLGLGMSS
                     IGQIGPAYYQNAKDLERYYAELDAGRLPVLRGLELTADDLVRRAVIQGLMCHFRLSIE
                     SIELAHLVDFRRYFAVELLQLKKLADDGLVELTPDWIGVTPKGRLLVRAICMLFDRYL
                     REARERASYSKVI"
     gene            9138..10442
                     /locus_tag="VIG70_17535"
     CDS             9138..10442
                     /locus_tag="VIG70_17535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY8252397.1"
                     /translation="MKVEGPGALTSVKRSVFRHPAVVAMGSAALVALLVLNGLAFVRG
                     LESPWLLGGVIISDMVVIGLSVLVAAREVLLADERTEASKTQLEAIVDSAMDAIITVD
                     EAQNIVLFNRAAEQVFRVRREEMLGTPLDRLLPTRFRGAHRDHIEGFGRTGVTSRRMG
                     DVTTLWALRAGSDEEFPIEASISQAAEGGKRFYTVILRDITLRKQAEDSLKESQRELR
                     ELSARVLEAREEEKAHIARELHDELGQLLTALKMDLGWVRERLPADSELAPRVAEMGT
                     LLDRTVGATRRISADLRPLMLDDLGLADAAAWLIDDFAKRSGVTCKVDIAEELPDLSK
                     AVATAVYRAVQESLTNIARHAGARSAWVVLAVEDDALYVEVEDDGRGIAPEDLAKARS
                     VGLKGMRERIAFLGGTFEIARAPRGGTRLRLRVPLRGLQEAA"
     gene            10454..11056
                     /locus_tag="VIG70_17540"
     CDS             10454..11056
                     /locus_tag="VIG70_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8252398.1"
                     /translation="MADDHSIVRDGLKRILAATTDLQVTGEAASGDQALALAKANDYD
                     VVMLDMSMPGLAGIDLIKRLKLEKPKLRILVLSMHGESQYAARALKAGAAGYLSKDSA
                     AEVLLGTLRKIAAGGVHIGEAAAAALLQTNDRLPHEALSDREFEVMRLLVEGLGPTEI
                     GERLHLSVKTVSTHKTRILEKLNLGSTAELVRYALEHRLA"
     gene            11289..11654
                     /locus_tag="VIG70_17545"
     CDS             11289..11654
                     /locus_tag="VIG70_17545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8252399.1"
                     /translation="MKVFLVEDAPLLRERLEALIASIPGTVVVGHADGAQGALRGILA
                     ERPDVVVLDIHLADGNGFDVLRGLRAAKFAPEIHVLTNYPLDGYRQTAERLGARGFFD
                     KSTEIDALRAALAARAASE"
     gene            11724..12092
                     /locus_tag="VIG70_17550"
     CDS             11724..12092
                     /locus_tag="VIG70_17550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8252400.1"
                     /translation="MEIFLVEDSPAVRGRLAELLEAVPGASVVGQAGRADTAIRDILS
                     LRPDVVLLDINLAEGSGFDVLRAVCRQAPEIDFYMLSNFTADPYRQLAERLGAREFFD
                     KSREIERVREIVAQRAAVHP"
BASE COUNT         1892 a         4310 c         4164 g         1860 t
ORIGIN      
        1 cgtggcgtag accatgcctt cgcgccccat gaagccgatg cagcgcgctt catcgaccag
       61 cacgtctttc gcgaacgaaa gacctttctg cagcgacccc ttcacggcgc cttgtagccg
      121 gcgcgctcga ccgctttctt catctgcccg acgtcgactt tcgccgggtc ataggtcact
      181 ttcgcgctcg cctgcgcgag cgagacgtcg gccttggcca cgccggccag gcccgacagc
      241 atgcgggtca cgctcttcac gcagccctcg cagtccattc cttcgacctt cagctcgacg
      301 gtttccatcg gttcagccat agagagttgc tgaccaccga gaccgagctc agcgccatcg
      361 cggcgccggc gatcatcggg ttcagcatgc ccgccgcggc gagcgggatg ccgagcacat
      421 tgtagatgaa ggcgaagaac aggttctgcc ggattttcgc gagcgtggcg cgcgacaggc
      481 ggatcgcgtc ggcgatcccg gcgaggtcgt tcttcatcag cgtgacgtcg gcggcctcga
      541 gcgcggcgcc gctgccggcg gcgagcgcga agctgacgtc ggccgcggcg agcgcgggcg
      601 cgtcgttcac gccgtcgccg gccatgccga cgcggcggtt ctcgcgcttg agcttctcga
      661 tctcgagcag cttctcctcc ggcagcacgc ccgccttcca gcgcgcgatg cccaggcgct
      721 cggcgaccgc cttcaccgcc tcggcgctgt cccccgagac gatgaccggc tcgatcccca
      781 tgcgcgcgag gcgcgccacc gcctgcgccg aggtcgctcg cagctcgtcg ccgagcgcga
      841 tccagccgag gaagcgcccg tcccattcca cgccgacgac gctcttgcct tcgacggact
      901 tctctcttcc caggaacgca ggcgagccca ggcgcgccgg ctttccgccg acgacgccgc
      961 tgaccccgcg cccgccgtgc gcgcggaact cggccacggg ctgcggcgcg ccgccgaacc
     1021 taacgacgat cgcgcgcgcg agcgggtgcc cggaccggct ttcgagcgcc gccgccacgc
     1081 gtagaagttc atcaggattg ctggaaaaaa catcggtgac cgcaggctca ccgcgcgtca
     1141 gcgtgccggt cttgtcgagc accagcgtgt cgagcgcccc cgcgccttcg agcgccgcgg
     1201 cgttgcggat caggatgccg gcgcgcgccg cgcggcccac gccgacgatt agcgcagtgg
     1261 gcgttgctaa cccgagcgcg cacgggcagg cgatgacgag cacggcgacc gcggggagga
     1321 tggcaatcgc aaagccgccg agccaccacc agaagagaag cgtcagcacc gaaacgccca
     1381 tgacgaccgg cacaaacacc gccgagaccc ggtcgaccag gcgctgcacc ggcggcttgg
     1441 aagcctgcgc cgcagcgacc aggcggatga tgccggcgag catcgtggcc ttgccggtcg
     1501 cggtcgcgac gcatttgagc aggccctcgc cgttgatcgt gccggcaaag accgcgtcgc
     1561 cgatgcgctt ttccttgggc acggactcgc ccgtgagcat cgactcgtcg accgcggagc
     1621 tgccgtccac cacccgggcg tcgagcgcca cggcgtcgcc cgggcgcacg acgaacgcgt
     1681 cgccggcatt cagggccgcg atgtcgacct cgacgagcgc gttattccgc tcgacgaacg
     1741 tcgttttcgg ttgcagccgg atcagggagt cgagcgcctg cacggccttc gccttggcgc
     1801 gcgcctcgag gtacttgccg agcagtacga gcgcgatcac caccgcccct gcctcgaagt
     1861 agagctgctc gttcaactga aaaaacagga ccacagcgga aaagccgtac gccgcgctcg
     1921 tgccgagccc gaccagcacg tccatgttcg cggtgccgcc gcgcagcgcg ttccacgcgc
     1981 cgcggtagaa gcgccggccg ctccagagct gcaccggcgt ggcgaggatg cactgcgccc
     2041 agccggggac aaacccgtgc aggagcagcg gcgccgtgag gacggcggca agcacgaact
     2101 cgtttctcgc cttgcggtgc tcggcggagt ggtcgcgcag ctcgccctcg cgcgcgaccc
     2161 gggcgtcgta accggtgtcg cggatcctct gaataatgga ttcaacctcg agcttcgaat
     2221 cgaagcgcac cgtggccttg tcggtggcga ggctgacgct cgcctccacg ccctcgagcc
     2281 tgttcaggtt cttctcgatg cgcgccgcgc aggcggcgca ggtcatgccc tcgatgtcga
     2341 gctggaccac gttcatgcgg cgatcttact cacagccagc ggccggcgcg ctgcgccgca
     2401 cgcaggccct ccaccgtgtc gacgccctgg cgcttcgcct cggcgagcgc gacgagcagg
     2461 agctgcgcgg tggagaacgc gtcgccgagc gcatcgtggc gctcgtgcac agcaatgccg
     2521 aattcggcga gccagtcgtc cagcgccttc gcttttcgcc cgggaaagag cgccggcgcg
     2581 agcgccgcca ggtcgagcca gcggcggcgc gcgaagccgt gccggcgcag caccgtcgca
     2641 tcgaaatccg cgtgaaagcc gaccgggatc gcatcatcga cgtgcgcgac tagtttctgg
     2701 atcacttgca gcagaggcaa gccggccagt tgcgcatcgc cgccgatgcc gtggatgagg
     2761 atgttgtccg cggtgctggg cgcgcgctgt tttacccggg cctcgaagca atccgagaga
     2821 tcgatgcgag catccctcac cgcgacggcg ccgaccgaaa gcagccggtc gcgtgcggcg
     2881 tccaggccgc tggtctcgca gtcgatcgcc acccagcgcg ccgccgcgat cggcgtgcgg
     2941 cgaaaccagc tcacagggcg aaatcgagcc gcaagcgctg ctgcagcatg gctgcctggc
     3001 ggaacgcctc cttcagcacg cgccggtcga tgtcgttcag ctcggcgcgc aggatgaggt
     3061 tgcgcgcatg ccgcagccgc agcgtctgga cgaaatggaa ggcctcgatc gcgtttttct
     3121 cgcccgcggc ccgcaggcga gccgcggtgc cggtctccgg cgacccggcg gcaagcgcga
     3181 gcacgcgcgc ggcatcgacg aacggccgcg cgccgagcgc cttgaggtcg agctcgtcgg
     3241 cggagaagtc ggcgagcagc ccgagcggcg gccgcgtctg cagcgccgtc tcggcgagcg
     3301 cgcggcagaa cgcgcggtgc gcgcgggtct gcgcgaggat tcccgagcgc agcgcgcccg
     3361 ccaggcgcgc atcgcccgcc agcgcccgga aatcgaagaa cacgctcgcg ttgagcagcg
     3421 cctggggatc gtggttgcgg atccagtcgt cgaacttctc gcgccactcc tggagcgtga
     3481 ggcaccagcg cggattgcgc gccatgatgt cgcccttgca cagcgggaag ccgcaggcgt
     3541 cgaggtcgcg gttcacctgg tcggcgaacg cgaggaacgc ctccttttcg ccttccatga
     3601 tgagcgcgtt gtcctggtcc gtcaccagcg tctgctccat ccggccctcg ctgcccagcg
     3661 cgagccagca ccacgcgggc gcgagcgggt gccgcgcctg ggcgagctcg atcacgcggc
     3721 gcgtcaggct gtcgttgagc gcgctgatca tttcggtgag cggcccggcc gccacgccct
     3781 gagcgagcag ctggcgcgcg aggcttcgga tgtcgcgcgc ggcctccacc agcggttcga
     3841 gccggcccgc gccgcggatg cgctcggcgg tgcgccgcaa gcccaggcgc tgcagcgcga
     3901 acagatcgcg ctccgacacc acccccgcga gcttgccgtc gcgcgtcacc acgatatggc
     3961 gaaaaccgtg ccgcgccatc gcgagcgcgg cgtcggcgag cgtcgcctcc tcctccagcg
     4021 cgaacggccg cggcgtcatg tgctgcgcga tgccggcgtc aagcggagtc ccggccgctg
     4081 cgctcgccag caggtcgacg gtggtgaaga tgcccacggg cacggcgtgg tcgtcgacga
     4141 tcgcgatcga acccacgtgc tcggcatgca tgcgccggat cgcctcgccg agggtcgtgt
     4201 cccgcgggca gctgaccggc tcgcgccgca acacgctcct cagcggcgcg agcattccgg
     4261 cgccgtcgag cgcggactcg gcggcctcgg cccgcagcgc gcgctgcgag cgctcggcga
     4321 gctgcgcgag gtgctgcgtg gagaaggcgc gaaaccgggc gctgcgctcc acgagctcgt
     4381 ggaaacgctc cgccggcagc tcccagcaga agctgtcctt ctcggcgcgg taggaataga
     4441 ccgtcgcgcg ccggcccgcc agcgcaccga gcggaaagca ctcgcccggg ccgagcagca
     4501 cgtcggcagc gcccccgctg ccgcgaacgc tgccctgctt cacgatgtag aggcgcgcga
     4561 cggcgccgct ctccggcccg accaccagct cgccgcgcgg gtagtagccg accgcgagat
     4621 ggccggcgag gaactggagc gccgcaggct ccatctcgtc gaacgggggg tgcgcgcgca
     4681 gcggcgcgat caggtctgcc acgccggcag attaagctag gcttgggccg cctgcctgcg
     4741 cgccgccatg cgctctccca gcgccgcccg ctgcgcgggc ccgatcgcct ggcgcgcgta
     4801 cggcagcagc aggtcgccct ccatgcgcat gtggcggcgg tggatccagg cgaagcgcgc
     4861 cacgagctcg gcatcgagcc gtgcggattc tccaacggcg atcgcctgca gctcggcgcg
     4921 tacctgcgca tacaggcggt ccatgctttg atgctcgcgc tgcagctcgt agagcgtcgc
     4981 gcagatctcg tcgcggccgt cgcgcgcggc ggcctcgcgc aacagcggaa acagatcctc
     5041 gtcctcgtcg cggtgatggg cggcggcgac ggtgtcgaag tagcgcatca ccgcgctcgc
     5101 cgcgctgcgg gccgcttcgt cgctgccacg ggcggcaagg tgggccgcga agttctcgag
     5161 cacgccaagc tcgaactcga tcccctggtg gcaggcctgc agcgtgtcca gcggctcgtc
     5221 catgctcaag ctgccgcggc tgcaatgcgg cgcgcggcgc cttcgaggtc gctgcgctcg
     5281 gcttgcgaga ggacgaagca gccctgtggc gcaacgccct cgcgcgcgag ccacgcccgc
     5341 gcgcaggcct tttcgcaggc gtccaggcaa aagaccggga atcgagattt cgccttcgcg
     5401 cgcgcagccg ccggttcagc ggctccgccg agccacgccg gctcgccgaa gcccaggcgg
     5461 tcgaggcgca gcgccacgtc ccgcgccgcc tggccgaatt cggcgcatcc ctggcaggcg
     5521 acgagcaccg gaaacggcct gaggacgacc gcggaaggcg accgggcgct caccggtaga
     5581 cgagcgtcgg aatcgtcgaa tgcgacagca cgtcgcgcgt cgcgctgccg aggaacagcg
     5641 ccggcaggac cttgcgtccg tgcgacgaca tgaagatgag atcgcagccg tggcgcgtcg
     5701 ccgccgcgat gatcgcctcg tagggctggt tgctctgcgc gtagtcgatc gcgcaggtaa
     5761 tcccggccgc gcgcgccttg cgcgccggct ccgccaggat cttctccgcc agcgcgcgcg
     5821 agcggttgtt gtgcgcttcg agcgatatgt cgtgcctctt gctcaacaac tcgccttcgc
     5881 ccggcacctc gtattcgggc accgcggtga accaggtgac ccgtgcgttc gcgtcccggg
     5941 cgaacgcgat gcccgcctcg accgccttct cggcggtttc cgatccatcg gtgggaatga
     6001 gaatgtgctt atacatcgat cgctcctttc tgctgtgccg cccgcttccc ttcgaaaacc
     6061 tcttcccagt ccacgttcgg gtcgggcgtg cgctcgagca ggcgttccac cagccggacg
     6121 tgctcctgtt cttcggccgc catctctctt gccagggcgc gcagcgcggg atcgtccgcg
     6181 gtgatgagga cccgctcgaa aaaggcctgc gcgcgcttct cgcccgccag cgcgatctcg
     6241 agcgcgtgcc gcggcgtcat caggcggaag accagctcgc gcgccgccgt ttccggcgcc
     6301 gccgcatcga gccactggta ggcggtcgtg gacgggtgcg gcagggccac gccccgcgtt
     6361 cgccgcagca gcgagtccaa gtgctccgcc tcgatctccg ccagccggcc gaacagctcg
     6421 gcgagcgcaa cgttccccag gtcgttcatg cgctcggcga actcggcata acgctcggcc
     6481 gcctcgcgct cgatggcgat ggcgtgcgcg taaagctcgg gcgcggttcg gatgggtgtc
     6541 atggattcaa tgtacggcgc gcggcaggag ccggcttgac ccagatcaaa tcacggcccg
     6601 ggcaacggcg tagcttcggc cccatgcact atcactatct gacgatcgag cagcgcgagt
     6661 acctggagaa gctgatccgc tcccgcgtgc gcggagcatc gcagctcgag accgcgctcg
     6721 aaaagctgcg tcagccggac tacggcacct gcatcgaatg cggaaaggac gtcgactacg
     6781 cccggcttca aatcgagccc ttcgccctgc actgccttgc gtgcagccgg ctgccgatcc
     6841 cggccggaac ctgaagatgg atcgagccat gccctatccg ctcgactacc agctctcgct
     6901 cgagcgcacg cttcgcatac ggctcgatgc ggtgcgcgcc gccggccgcg catttgagac
     6961 atgcatcctc gaagagaccc tggcacggct gcgcagcgcg gagttcggca cctgcctcag
     7021 gtgcggcggc gtcattccgt tcctggaaat ggccgcggac ccgacgcggc agacctgccg
     7081 ttcgtgcacg cgctaacgaa gatcccggtc ctcgtcaccc gctagcgccc tgctacacgc
     7141 gcttccaggg ctcaccgagc ccgaccggcg ccggcgccag gttggcgatg cgcgaaaaca
     7201 gcttctcgaa attgcgctcc tgcacctcgg cgatcgggtc ggcgttggcc gcaaacgccg
     7261 cgtcgatcgc cttccagtcc acggccgtga ggtggcgctg cgcgagcggc agcagctcgc
     7321 tctcctcctt gcgcatgtgc ttccagtgga actcggcgta agcatccacc gccgctagga
     7381 attcgcgcgc gccggcgggc cagccctcct cgaggaacac cagcgcgcgc tcgagctcgc
     7441 ggatgagccg cgcgccttcc tcgtgctcag cctgcagctc gttcaccagc ggccgcgtct
     7501 cgggcgcgcg cgccaccagg cgcgcgaaga gatgctcgtc ctccttcggg tggtgcagct
     7561 tctcgggata ttcgtcgatg tagcgcagca tgctgcgcag cacctcgaag cgcggccgca
     7621 cccgggcgtc ctgcgccatg cgggccagct ctttcaggcc cagcagcacc gccgagatcg
     7681 agcgatgctc gctcttcatg atcgtgatcg cgtctttcat gtcgttctcc cttgctcata
     7741 tcactttgga atagctcgcg cgctcgcgcg cttcgcgcag gtagcggtcg aacagcatgc
     7801 agatcgcgcg caccaggagg cgccccttcg gcgtcacccc gatccagtcg ggcgtgagct
     7861 ccaccaggcc gtcatcggcg agctttttga gctgcagcag ctcgaccgcg aaatagcgcc
     7921 ggaaatcgac gagatgcgcg agctcgatcg actcgatcga caggcggaaa tggcacatca
     7981 ggccctggat caccgcgcgc cgcaccaggt cgtccgcggt gagctcgagg ccgcgcaaga
     8041 ccggcagccg gccggcatcg agctcggcgt agtagcgctc gaggtccttc gcgttctggt
     8101 agtaggccgg gccgatctgg ccgatcgacg acatgcccag cccgagcaga tccccggcct
     8161 gcgtggagta gccctggaag ctgcgcgcga gctggccgag cgtctgcgcc gccgcgagct
     8221 cgtcgtcggg cctcgcgaaa tggtccatgc cgatgtagag gtagcccgcg cgcgtcaggc
     8281 gtccgatggc gagcgtcatc agctgcagct tgcgctcggc cgacggcagc tcggactcgg
     8341 cgatgcgccg ctgcggcttg aacagcttcg gcaggtgcgc gtagttgtag agcgcgatgc
     8401 gctcggggtc gaggagcagc acctcgtcga gggtgcggtt gaagctgtcg agcgtctgct
     8461 tcggcagccc gtagatcagg tccaggttca ccgagcggaa gccgctcgcg cgcgcctcgg
     8521 ccatcacgcg ccgggtcgtc tccacgctct gcagccggtg caccgccttc tgcaccgccg
     8581 cgtcgaaatc ctgcacgccg agcgacaccc ggttgaagcc gagcccggcg agaaacgcca
     8641 tgcggccggg ctcggcgctg cgcgggtcga cctcgatcga gtactcggct ttcggattgc
     8701 gcgcgaacct ggattcgatg gcgcgcacga gcgcgctcga ttcgtcgcgc gtgagaaacg
     8761 taggcgtacc gccaccccag tgcatcgcct ggatcggaca ggactcgccc agcagcggcg
     8821 caagcagcgc cagttccttc tccaagtact tgatatactt ggccgatctg ccgtggtcgc
     8881 gagtgaccac cttgttgcag gcgcagtagt agcagacgtt ctcgcagaac ggcaggtgga
     8941 cgtagaggga gagcggctgg ctgaagccgc cgatgttgcg cttggcgagc cactgccgga
     9001 gctgcggctc gccgaacgcc tcaacgaagc ggtccgccgt agggtacgag gtgtagcgcg
     9061 gaccgctcac gtcgtacttg cggatgagca ccggatcgat gaccagctcg gaggcggggt
     9121 agtgcattgg gagcactatg aaggttgagg gcccgggggc tttgacctcg gtcaaacgaa
     9181 gcgtcttccg ccacccggcg gtggtggcga tggggtcggc cgcgctagtc gcgctcctcg
     9241 tgctgaacgg gcttgcgttc gtgcgcggcc tggagtcgcc ctggctgctc ggcggcgtga
     9301 tcatctccga catggtggtg atcggcctca gcgtcctggt tgcggcgcgc gaggtgctgc
     9361 ttgcagacga gcgcaccgag gcgagcaaga cgcagctcga ggcgatcgtc gactcggcga
     9421 tggacgcgat catcacggtc gacgaggcgc agaacatcgt gctgttcaac cgcgccgccg
     9481 agcaggtgtt ccgcgtgcgc cgcgaggaga tgctcggcac gccgctcgac cggctgctgc
     9541 cgacgcgctt tcgcggtgcg caccgcgacc acatcgaggg cttcggccgc accggcgtca
     9601 ccagccgccg gatgggcgac gtcaccaccc tgtgggccct gcgcgccggc agcgacgagg
     9661 agttcccgat cgaggcttcg atctcgcagg cggcggaggg tggcaagcgc ttctacaccg
     9721 tgatcctgcg cgacatcacg ctgcgcaagc aggccgagga ctcgctgaag gagtcgcagc
     9781 gggaattgcg cgagctttct gcaagggttt tggaagccag ggaagaagaa aaggcgcaca
     9841 tcgcgcgcga gctgcacgac gagctcggcc agctcctgac ggcgctgaag atggatctgg
     9901 gctgggtgcg agagcggctg cccgccgatt cggagctcgc gccgcgcgtg gccgagatgg
     9961 gaacgctgct cgaccgcacg gtcggcgcga cgcggcgcat ctcggccgac ctgcgcccgc
    10021 tgatgctcga cgacctcggc ctggcggacg ccgcggcctg gctgatcgac gatttcgcca
    10081 agcgctcggg cgtgacctgc aaggtcgaca tcgccgaaga gctgcccgac ctttccaagg
    10141 ccgtcgccac ggcggtgtac cgcgcggtcc aggagtcgct caccaacatc gcgcgccatg
    10201 ccggggcaag gagcgcctgg gtggtgctcg cggtggagga cgacgcgctc tacgtcgaag
    10261 tagaggacga cggccgcggc atcgcgcccg aggacctcgc caaggcgcgc tcggtcggtc
    10321 tcaagggcat gcgcgagcgc atcgcgttcc tcggcggcac gttcgagatc gcacgcgcgc
    10381 cgcgcggcgg cacgcgcctg cggctgcggg tgccgctgcg tgggttgcag gaggcagcct
    10441 gatccgcgtt ctgatcgccg acgatcacag catcgtgcgc gacggcctca agcgcatcct
    10501 cgccgcgacc accgacctgc aggtcaccgg cgaagcggcg agcggcgacc aagcgctcgc
    10561 gctggcgaag gcgaacgact acgacgtcgt gatgctcgac atgtcgatgc ccggcctcgc
    10621 cggcatcgac ctcatcaaac gcctgaagct cgagaagccg aagctgcgca tcctggtcct
    10681 gtcgatgcac ggcgagagcc agtacgccgc gcgcgcgctc aaggccggcg ccgcgggcta
    10741 cctcagcaag gacagcgccg ccgaggtgct gctgggcacg ctgcgcaaga tcgccgcggg
    10801 cggcgtgcac atcggcgagg ccgcggccgc ggccttgctt caaacgaatg acagattgcc
    10861 gcacgaagcc ctgtcggacc gcgagttcga ggtgatgcgg ctcctggtgg agggcctcgg
    10921 cccgaccgag atcggcgagc ggctgcacct ctcggtcaag accgtcagca cgcacaagac
    10981 ccgcatcctc gagaagctca acctcggcag caccgccgag ctcgtccgct acgccctcga
    11041 gcaccggctc gcctagggaa acctaggttg atgtagatca atcggatggc gcgattgccg
    11101 cttatgctcg tcgggcaggc tttccttgat gaaccaggtc tatgccgcgg tcatcctgat
    11161 cctcgccctt tgccttgtgg cgctggtggc ccgattcttc ggcatcctgt agtcggaaaa
    11221 ttcctacccg ttatcgcggc ggcggactac agaccgccaa gcaggccgtc gggatactcc
    11281 caggggtaat gaaagtattc ctggtggagg atgcgccgct cttgcgcgag cggctggagg
    11341 cgctgatcgc ctcgattccg ggcacggtcg tcgtcggtca tgcggacggc gcgcagggag
    11401 cgctgcgcgg catcctcgcc gagcggcccg acgtcgtggt gctcgacatc cacctcgccg
    11461 acggcaacgg cttcgacgtg ctgcgcgggc tgcgcgcggc gaaattcgcg cccgagatcc
    11521 acgtgctcac caattacccg ctcgacggct accgccagac cgccgagcgg ctcggcgcgc
    11581 gcggcttctt cgacaaaagc accgagatcg acgcgttgcg cgccgcgctc gccgcgcgcg
    11641 cggcgagcga ataggaaagc gcctaccctg attatcagcc gccgccgata gggcgcgcgg
    11701 cgggcggccg gcacactgcc ggcatggaaa tttttctggt tgaagattca ccggccgtgc
    11761 gggggcggct tgccgagctg ctcgaggccg tgccgggcgc gagcgtcgtg ggccaagccg
    11821 ggcgtgccga caccgcgatc cgcgacattc tttcgctcag gcccgacgtg gtgctgctcg
    11881 acatcaacct cgccgaaggc agcggcttcg acgtgctgcg cgcggtgtgc cgccaggcgc
    11941 ccgaaatcga cttctacatg ctgtccaatt tcaccgccga cccctaccgc cagcttgccg
    12001 agcggctcgg agcgcgcgag ttcttcgaca aaagcaggga aatcgagcgc gtacgcgaga
    12061 tcgtcgccca gcgcgccgcc gtccacccgt gaagggagcc gccatgaccg ccgccgtgat
    12121 cgccctcaag ccgaccttca agcccatgaa caaccacaag ttcgaagtga gttgcgcgag
    12181 ctgcaacctg cgcgagatct gcctgccggc ggggctgtgc ctcgac
//
LOCUS       DATBSZ010000271         5977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1282161, whole genome shotgun sequence.
ACCESSION   DATBSZ010000271 DATBSZ010000000
VERSION     DATBSZ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282161"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VIG70_17555"
     CDS             complement(<1..152)
                     /locus_tag="VIG70_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY8252401.1"
                     /translation="MFTGIVQAVGRIVRTHPLEVDSGSLELAGVAVGDSICVQGVCLT
                     VTGLRPR"
     gene            complement(183..548)
                     /locus_tag="VIG70_17560"
     CDS             complement(183..548)
                     /locus_tag="VIG70_17560"
                     /inference="COORDINATES: protein motif:HMM:NF015720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HEY8252402.1"
                     /translation="MVAKWQDWASFVLGLWLALSPWLAGYVEHEAATANAIFVGLALA
                     LCSHFECVACDEMPAEWMNLAAGVWLICAPFVLEFTSSAMGSANSVLVGGCVSALAVS
                     ALALDKHLGRLLHKRVVRL"
     gene            655..1557
                     /locus_tag="VIG70_17565"
     CDS             655..1557
                     /locus_tag="VIG70_17565"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEY8252403.1"
                     /translation="MKLQIELIGAAWGLGGADPGCAEAPRVLAPLLEQRLAECGAPPV
                     LGPILEPAAGERRKQFAVSRLCAELAAAVAAARRRDRLPCVIGGDHSCAGGTWTGVAR
                     TLRGSLGLLWIDAHMDSHTPATSHTGRLHGMPLAWLLGQDDDPLYGLSSGVLEPQHVC
                     LVGVRSYEPEEDERLRRLGVRVVFMDEIASRGIDAVLDEALDIVTAGTGGFGVSIDLD
                     VVTPEEAPNVGTPAKGGVSSAELARALERIPLRPGLAAVELVEYSPRLDRDGRTARVA
                     IELLSAALCGPRHDAQVLAEAIQR"
     gene            complement(1504..2544)
                     /gene="ribD"
                     /locus_tag="VIG70_17570"
     CDS             complement(1504..2544)
                     /gene="ribD"
                     /locus_tag="VIG70_17570"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY8252404.1"
                     /translation="MMRRALELAETGLYTTTPNPRVGCVITREEKIVGEGWHERAGGP
                     HAEVVALEKAGSAAVGATAYVSLEPCNHRGRTPPCVDALKKAKVKRVVAAMRDPNPKA
                     SEGGKALEAAGIRFEHGLMEDEARELNIGFVSRMTRGRPWVRLKVAATLDGRTALRDG
                     QSQWITGAEARRDGHRWRARACAILTGIGTVRADDPRLTVREVETPRQPLRVIVDSRL
                     ETPPAARILDGGNVLIFSGKPGSAPRGAEVVVLPNANQKVELPKMLEELARRGVNELH
                     VEAGFRLNGSLAREGCVDEYLVYLNPSFLGDGAQGMLDLPAFDSLEKKLKLRVLSLDR
                     FGEDLRIVARPA"
     gene            complement(2583..3050)
                     /gene="nrdR"
                     /locus_tag="VIG70_17575"
     CDS             complement(2583..3050)
                     /gene="nrdR"
                     /locus_tag="VIG70_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY8252405.1"
                     /translation="MKCPFCGSDDTQVVDTRANLEANTVRRRRKCVACEKRFTTYERV
                     DLKMPRLVKKDGSRTDFDRDKLMGSMMLALRKRPVATDEVEGAVDRVIERLRSLGERE
                     VPTNRVGDFVMRELAKLDKIAYIRFASVYRSFESPDDFREAVQEVKAGKRRRK"
     gene            complement(3047..4288)
                     /gene="glyA"
                     /locus_tag="VIG70_17580"
     CDS             complement(3047..4288)
                     /gene="glyA"
                     /locus_tag="VIG70_17580"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY8252406.1"
                     /translation="MLAPDRLAQSDPEVWNAIRLEYQRQEEHVELIASENYASVAVLA
                     AQGTALTNKYAEGYPGKRYYGGCAFVDVAEHLAIERAKRLFGAQYANVQPHSGSQANQ
                     AAYLAFLKPGDTILGMSLAHGGHLTHGAAVNASGKLYHAVSYGLDREEVLDYAEVRRL
                     ALEHNPRVIVAGASAYSLAIDWQRLRHIADEIDAVLMADIAHYAGLIAAGLYPTPVGI
                     ADVVTSTTHKTLRGPRGGMILAEPEHERALNSAVFPGIQGGPLMHVIAAKAVAFGEAL
                     KPEFRDYQQRVIHNAQVMVEILKKRGLRIVSGRTECHLFLVDLRAKQITGKDAEEALG
                     RVGITLNKNAIPNDPQKPTVTSGIRIGTPAMTTRGFGETEAETLGHLIAEVLDDPTGD
                     GTARRVAAEIKALCQRFPVYA"
     gene            complement(4317..5225)
                     /locus_tag="VIG70_17585"
     CDS             complement(4317..5225)
                     /locus_tag="VIG70_17585"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8252407.1"
                     /translation="MASKRVAVLFGDVCDSTTIYEAIGDTRAHALITRLLARLDEAVK
                     ASAGTVVKTLGDGLVCHFKDADSAFHAACQMQEWAAAMSEGAEPKLSIKVGFTWGPVV
                     TKAGDIFGDTVNVCARLVSVAGPAQVLTTQEAVDALSEPLRSRCRQLYPMKVKGRVSD
                     VNVCDVQWRDDDLDQTAGLSRSGMMRTRPRGEWALKLSYGGDSVVVEPGASISIGRDK
                     GNDMVVNSTHASRVHARVHGRGAHFVITDQSSNGTFVLIDGHTRELQLRRDEAVLGER
                     GYIGLGDSASKQGDHVVRYRLENRKP"
     gene            complement(5228..5824)
                     /locus_tag="VIG70_17590"
     CDS             complement(5228..5824)
                     /locus_tag="VIG70_17590"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY8252408.1"
                     /translation="MRSQASLKIVGLLLAAGAASRFGSDKLRHALPHGVSIAVQAARH
                     LKMEIPKVVAVVRPGSDELAQALKNEGCEVVVCKNAADGMGASLACAAGAAQEADGVL
                     IALADMPYVRPTTIAAVRDALVAGAPLAAPYFRARRGHPVALSRKFLGQLLALKGDEG
                     AKQLVAENERALVKIPVGDPGVIRDIDTPDDLAPPLRI"
     gene            complement(5803..>5977)
                     /locus_tag="VIG70_17595"
     CDS             complement(5803..>5977)
                     /locus_tag="VIG70_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY8252409.1"
                     /translation="ARLRGPVGLYIGSKTPPEIALAILAEMTAVRHGVREPSWAAKPE
                     RRFDPSACEVKQA"
BASE COUNT          870 a         2096 c         2029 g          982 t
ORIGIN      
        1 cggggtctca agcccgtcac cgtgaggcac acgccttgca cgcagatcga gtcgccgacc
       61 gccacgccgg caagctcgag gctgcccgag tcgacttcga gcggatgggt gcgcacgatg
      121 cggccgaccg cctgcacgat gcccgtgaac atggcgccga agcctacccc aaaaccgatt
      181 gtctaaagcc tgacgacccg cttgtgcaga agcctgccga gatgcttatc cagcgccagc
      241 gccgacaccg ccagcgcgga tacgcaaccg ccgaccagca ccgagttggc gctccccatc
      301 gcggagctgg tgaactccag gacgaaaggt gcgcagatca gccagacgcc cgcggcgagg
      361 ttcatccact ccgcgggcat ctcgtcgcag gcgacgcatt cgaaatgcga gcacagcgcc
      421 agggcaagcc cgacaaagat ggcgttcgcg gtcgccgcct catgctccac gtaccctgcg
      481 agccagggcg agagcgcgag ccaaagaccg agcacaaagc tcgcccagtc ctgccatttc
      541 gccaccatcg cgctctcctt gacgcgcgcc cgatgcgcgc tccacgattg gacaccgatg
      601 gcgttgcgcc cgttctattc cgggcgtagt ccttcccggg tacagcgtgt cacgatgaaa
      661 ctacagatcg aactgatcgg cgcagcatgg ggcctgggcg gcgcggatcc gggctgcgcc
      721 gaggcgccgc gggtccttgc gccgctgctc gagcagcggc ttgccgaatg cggcgcaccc
      781 ccggtcctcg ggccgatcct cgagccggcg gcgggcgagc gacgcaagca gttcgcggtg
      841 agcaggctgt gcgcggagct ggccgctgcg gtcgccgcgg cgcgccgccg cgaccggctg
      901 ccctgtgtca tcggcgggga ccacagctgc gccggcggca cctggaccgg cgtggcgcgc
      961 acgctgcgag gaagcctcgg cctgctctgg atcgacgcgc acatggacag ccatacgccc
     1021 gccaccagcc acacgggtcg cctgcacggc atgccgcttg cctggctgct cggccaggac
     1081 gacgacccgc tttacgggct ttcctccggc gtgctcgagc cgcagcacgt ctgcctcgtc
     1141 ggcgtgcgca gctacgagcc cgaggaagac gagcgtctgc ggcgcctcgg cgtgcgggtc
     1201 gtgttcatgg acgagatcgc gagccgcggc atcgacgcgg tgctcgacga agcgctcgat
     1261 atcgtcaccg cgggaaccgg cggcttcggc gtcagcatcg acctcgacgt cgtcacgccc
     1321 gaggaagcgc cgaacgtcgg cacgccggcg aagggcggcg tgagcagcgc cgagctcgcg
     1381 cgcgcgctcg agaggatccc gctccgtccc ggcctggccg cggtggagct ggtcgagtac
     1441 agcccgcgcc tcgatcgcga cgggcgcacc gcgcgggtgg cgatcgagct cctgagcgcc
     1501 gccctatgcg ggccgcgcca cgatgcgcag gtcctcgccg aagcgatcca gcgataggac
     1561 tctcagcttc agtttctttt cgagtgagtc aaaggccggg aggtcgagca tgccttgcgc
     1621 cccatcgccg aggaagctcg gattgagata aaccaggtat tcgtcgacgc agccttcgcg
     1681 cgcgagcgag ccgttcaggc gaaagcccgc ctccacatgc agctcgttca ccccgcgccg
     1741 tgccagctcc tccagcatct tcggcagctc gactttttgg ttcgcattgg gcagcacgac
     1801 cacctcggcg ccgcggggcg cgcttcctgg cttgcccgaa aaaatcagca cgttgccgcc
     1861 gtccaggatg cgcgccgctg gcggcgtctc tagccggcta tccacgatga cgcggagcgg
     1921 ctggcgcggc gtctcgactt cgcgcacggt gaggcgcgga tcgtcggcgc gcacggtgcc
     1981 gataccggtc aggatcgcgc acgcgcgcgc gcgccagcgg tgaccgtcgc ggcgggcctc
     2041 ggcgcccgtg atccattgcg attgcccgtc gcgcagggcc gtgcggccgt cgagcgtcgc
     2101 cgcgacctta agccgcaccc agggccggcc gcgcgtcatg cgcgagacga agccgatgtt
     2161 cagctcgcgc gcctcgtcct ccatcaggcc gtgctcgaag cggatgccgg ccgcctcgag
     2221 cgcctttccg ccctccgacg ccttcgggtt cggatcgcgc atcgccgcga ccactcgctt
     2281 cacttttgct tttttcagag cgtccacaca aggcggcgtg cggccacgat ggttgcacgg
     2341 ctccaggctc acgtacgccg tggcgcccac tgccgcgctt cccgcctttt cgagcgccac
     2401 aacttcggca tgcggcccgc cggcgcgctc gtgccagcct tcgccgacga ttttttcttc
     2461 ccgggtgatt acgcagccga cacgcgggtt cggcgtcgtg gtgtagaggc ccgtctccgc
     2521 caattcgagc gcgcgccgca tcatcgcgtg gtcgaacgcc gaaaaccggg tcactgagtc
     2581 tcttatttcc ttcttcgttt tcccgccttc acttcctgga ccgcctcgcg gaagtcgtcc
     2641 gggctctcga agctgcggta gaccgacgcg aagcggatgt aggcgatctt gtcgagcttg
     2701 gcgagctcgc gcatcacgaa gtcgccgacg cggttggtcg gcacctcgcg ctcgccgagc
     2761 gagcgcaagc gctcgatgac ccggtcgacc gcgccctcga cctcgtcggt cgccaccggg
     2821 cgcttgcgca gcgcgagcat catgctgccc atcagcttgt cgcggtcgaa gtcggtgcgg
     2881 ctgccgtcct tcttcaccag gcgcggcatc ttgagatcga cccgctcgta ggtggtaaag
     2941 cgcttctcgc aggcgacgca tttgcgccgc cgccgcacgg tgttcgcctc gaggttggcg
     3001 cgggtatcga cgacctgggt gtcgtccgat ccgcagaagg ggcacttcat gcgtagaccg
     3061 gaaagcgctg gcagagcgcc ttgatctccg ccgccacgcg ccgcgcggtg ccgtccccgg
     3121 tcgggtcatc gagcacctcg gcgatgaggt gcccgagcgt ctcggcctcc gtctcgccga
     3181 agccgcgcgt ggtcatcgcc ggcgtgccga tgcggatgcc ggaggtgacc gtgggcttct
     3241 gcggatcgtt cgggatcgcg ttcttgttga gcgtgatccc aacgcggccg agagcctctt
     3301 cggcgtcctt gccggtaatc tgcttcgcgc gcaggtcgac gagaaacagg tggcactcgg
     3361 tgcggccgga gacgatgcgc agcccgcgct ttttcaggat ctcgaccatc acttgggcgt
     3421 tgtggatgac ccgctgttgg taatccctga actcgggctt gagcgcctcg ccgaaggcca
     3481 ccgccttcgc ggcgatcacg tgcatcagcg gcccgccctg gatccccggg aacacggccg
     3541 agttgagcgc gcgctcgtgc tccggctcgg cgaggatcat gccgccgcgc ggcccgcgca
     3601 gcgtcttgtg cgtggtgctg gtgaccacgt cggcgatccc caccggcgtc ggatagaggc
     3661 ccgccgcgat caggccggcg taatgcgcga tgtcggccat cagcaccgca tcgatctcgt
     3721 cggcgatgtg gcgcagccgc tgccagtcga tcgcgagcga ataggccgag gcgcctgcga
     3781 cgatgacgcg cggattgtgc tcgagcgcga gccgcctgac ctccgcgtag tcgagcacct
     3841 cttcgcggtc gaggccgtag gacacggcgt ggtagagctt gccgctcgca ttgaccgcgg
     3901 cgccgtgcgt gagatgcccg ccgtgggcga gcgacatccc gaggatcgtg tccccgggct
     3961 tgaggaaggc gaggtacgcc gcctggttcg cctgcgagcc cgagtgcggc tgcacgttgg
     4021 cgtactgcgc gccgaagagc cgcttcgcgc gctcgatggc gaggtgctcg gcgacgtcga
     4081 cgaaagcgca gccgccgtag tagcgcttgc cgggatagcc ctcggcgtac ttgttggtga
     4141 gcgccgtgcc ctgcgccgcg agcaccgcga cgctcgcgta gttctccgag gcgatcagct
     4201 cgacgtgctc ctcctggcgc tggtactcga gccggatcgc gttccagact tcgggatcgg
     4261 actgcgccag ccggtcggga gcgagcattt tttgagggcg ggaaaagacg ccgattctaa
     4321 ggcttgcggt tctcgagccg gtagcgcacc acgtgatcgc cctgtttcga ggccgagtct
     4381 ccgaggccga tgtagcctcg ttcgccgagc actgcttcgt cgcgccgcag ctgcagctcg
     4441 cgggtatggc cgtcgatcag cacgaaggtg ccgttggagg actggtcggt gatgacgaag
     4501 tgcgcgccgc ggccgtggac gcgagcgtgc acccgtgagg cgtgggtgct gttcacgacc
     4561 atgtcgttgc ccttgtcgcg cccgatgctg atcgaggcgc ccggctcgac cacgacgctg
     4621 tcgccgccat agctgagctt gagggcccac tcgccgcgcg ggcgcgtgcg catcatgccg
     4681 gagcggctca ggcccgcggt ctggtcgagg tcgtcgtcgc gccactggac gtcgcacacg
     4741 ttcacgtcgc tcacccggcc tttgaccttc atggggtaga gctgacggca gcggctgcgc
     4801 agcggctccg agagcgcgtc gaccgcttcc tgcgtagtca gcacctgcgc cgggccggcc
     4861 accgagacga ggcgcgcgca gacgttcacg gtgtcgccga agatatcgcc ggccttggtc
     4921 accaccgggc cccaggtgaa cccgaccttg atcgagagct tcggctctgc gccttccgac
     4981 atcgccgccg cccactcctg catctggcac gcggcgtgga aggcggagtc ggcatccttg
     5041 aaatggcaca ccaggccgtc gccgagcgtc ttcaccacgg tgccggcgct cgccttgacc
     5101 gcttcgtcga gccgcgcgag cagccgcgtg atcagcgcat gcgcgcgcgt gtcgccgatc
     5161 gcttcgtaga tcgtcgtgct gtcgcaaacg tcgccgaaga ggaccgcgac ccgcttgctt
     5221 gccatcgtca gattctaagg ggtggcgcga gatcgtcggg cgtgtcgatg tcgcggatca
     5281 ccccgggatc gccgaccgga atcttgacca gcgcgcgctc gttctcggcg acgagctgct
     5341 tcgcgccctc gtcgcctttc agggcaagca gctgccccag aaacttcctt gacagcgcga
     5401 ccggatgtcc cctgcgcgcg cgaaaataag gcgcggcgag cggcgcgccg gcgacgagcg
     5461 cgtcgcgcac cgccgcgatt gtcgtgggcc gcacgtacgg catgtcggcg agcgcgatga
     5521 gaaccccgtc agcttcttgc gcggcgccgg cggcgcacgc gaggctggca cccatgccgt
     5581 cggcagcgtt cttgcacacc acaacctcgc agccttcgtt tttcagcgcc tgagcgagct
     5641 cgtcgctgcc gggacgcacg accgccacga ccttcgggat ctccatcttc agatgccgtg
     5701 cggcctggac ggcgatcgag acgccgtgcg gcagcgcgtg ccggagcttg tcggagccga
     5761 aacggctcgc cgcccccgcg gcgaggagca gcccgacgat cttcaggctt gcttgacttc
     5821 gcaggcggag gggtcaaagc gccgctcggg cttcgccgcc cacgagggct ccctcacgcc
     5881 gtggcgcacc gcggtcatct cggcgaggat cgcgagcgcg atctccggcg gggtcttgga
     5941 gccgatgtag agcccgaccg ggccgcgcag gcgcgcg
//
LOCUS       DATBSZ010000272         4409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1898390, whole genome shotgun sequence.
ACCESSION   DATBSZ010000272 DATBSZ010000000
VERSION     DATBSZ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898390"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /gene="glnA"
                     /locus_tag="VIG70_17600"
     CDS             <1..783
                     /gene="glnA"
                     /locus_tag="VIG70_17600"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451619.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY8252410.1"
                     /translation="HHHEVGAMGQNEIGTRFAPLVQRADWMQILKYTVWNTAASYGKT
                     ATFMPKPVVGDNGSGMHVHQSIWKGGKNLFAGNGYSGLSDLALHYIGGIIKHAKALNA
                     ITNPGTNSYKRLVPGFEAPINLAYSARNRSAACRIPYVSSPNGRRVEVRFPDPTANSY
                     LAFAAMLMAGLDGVQNKIHPGDPVDKDLYHLPPEEAKKVPNVCASLEQALGCLRDDHA
                     FLTRGGVFSEDFLDSYIELKETELTRFRMTTHPVEFDMYYSA"
     gene            866..1339
                     /locus_tag="VIG70_17605"
     CDS             866..1339
                     /locus_tag="VIG70_17605"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY8252411.1"
                     /translation="MYRSWAFGVVLVCGTAHAQQATTEIWRCIDADGRRHYTNAKKET
                     AGMKCELVTSQINVAPPLSQPKAGRTSPGFPRETPQQSVSARERQREILEGELAAERE
                     SLNQAKQALEAQEAVRSGDERNYARVQERLQPFKDAIQNHEKNIQALQRELGNLK"
     gene            1347..2372
                     /gene="glnL"
                     /locus_tag="VIG70_17610"
     CDS             1347..2372
                     /gene="glnL"
                     /locus_tag="VIG70_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEY8252412.1"
                     /translation="MAASFAGLDLLATAVVVLDDEFVVRYANPAAENLLTTGARSLIG
                     QPFLGFFAERTELERSLEDARVIHWDYSARNVTYVRVGRQPVPLSCTVTRIDSFGMAL
                     LAELRPISEQLRIEREARMESEQQANRELIRNLAHEIKNPLGGLRGSAQLLERELERP
                     ELREYTQVIIKEADRLQTLMDRMLTPHRAPRLEPLGLHEVLERVRSLVRAEFGIEIVR
                     DYDPSLPDILGDREQLIQAVLNIARNAAQVGSGSVTFRTRVLRQITILKKRYKLALEL
                     QVIDDGPGVPEEIQDRIFNPLVSGREGGTGLGLSLAQTFVHYHHGVIEFESRPGRTIF
                     RILLPIR"
     gene            2369..3784
                     /gene="ntrC"
                     /locus_tag="VIG70_17615"
     CDS             2369..3784
                     /gene="ntrC"
                     /locus_tag="VIG70_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEY8252413.1"
                     /translation="MKPVWIVDDDRSIRWVIEKALSREGIAYNSFSSAQEALDALSVD
                     APEVLVSDIRMPGRSGLELLQAVKQRHPAVPVIVMTAYSDLDSAVAAFQGGAYEYLPK
                     PFDIDQAVDLIRRALDERKRESEAAEPPAATPEILGQAPAMQEVFRAIGRLSQSSATV
                     LITGESGTGKELVARALHRHSARARQPFVAINTAAMPKDLLESELFGHERGSFTGAQQ
                     QRRGRFEQAEGGTLFLDEIGDMPSELQTRLLRVLSDGTFYRVGGHQQIKVNVRVIAAT
                     HQNLEQRVREGTFREDLYHRLNVIRLRLPSLRERSEDIPLLAKHFLASSAKQLGVEPK
                     RIADDALAHLARLEFPGNVRQLENLCHWLTVMAPGQVIDLGNLPPEFRAQVPGESVTA
                     ASDWLAALEQEAERRLARGDTGILDALGRQFERALIVKALARTGGRRIDAANLLGMGR
                     NTITRKIQELGIEDKGGQDET"
     gene            complement(3855..>4409)
                     /locus_tag="VIG70_17620"
     CDS             complement(3855..>4409)
                     /locus_tag="VIG70_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252414.1"
                     /translation="EEKINVLQVRLPTNFKSARFPGDAHTAILKQLEADIEAARFDKM
                     ELPVKLKVHESVFVPLAKWAMLIAGNYRCITREGPRAIKDAVHSDINASRATYQWVVN
                     LCLLLGAKESDLVPFDKYAAAAQSLGSPSSAARALYAGAPYIERVDRLVQAIAKQKGL
                     QLPVLDEVVGLVDAKLEANRKKAA"
BASE COUNT          806 a         1495 c         1404 g          704 t
ORIGIN      
        1 caccaccacg aggtcggcgc gatgggccag aacgagatcg gcacgcgctt cgcgccgctc
       61 gtgcagcgcg ccgactggat gcagatcctc aagtacacgg tgtggaacac cgccgcgtcc
      121 tacggcaaga cggcgacctt catgccgaag ccggtagtcg gcgacaacgg ttcgggcatg
      181 cacgtccacc aatcgatctg gaagggcggc aagaacctct tcgccggcaa cggctactcg
      241 gggctttccg acctggcgct gcactacatc ggcggcatca tcaagcatgc caaggcgctg
      301 aacgcgatca ccaatcccgg caccaactcg tacaagcgtc tggtgccggg cttcgaggcg
      361 ccgatcaacc tcgcttactc cgcacgcaac cgctcggcgg cgtgccgcat cccatacgtg
      421 tcgagcccga acggccgacg cgtcgaagtg cgctttcccg atccgacggc caattcctac
      481 ctggcgttcg ccgcgatgct gatggcaggg ctcgatggcg tgcagaacaa aatccacccc
      541 ggcgacccgg tggacaagga cctctaccac ctgccgccgg aagaggcgaa gaaggtgccg
      601 aatgtctgcg cgtcgctgga gcaggcgctc ggttgcctgc gcgacgacca cgcgttcctc
      661 acgcgcggcg gcgtgttctc ggaggatttc ctcgactcgt acatcgagct caaggaaacc
      721 gagctcacgc gcttccgcat gaccacgcac ccggtcgagt tcgacatgta ttactcggcc
      781 tgattgtgca gatgcacagg aaaaggggcg gtgcgaggta acccgtggcg ccgccccttt
      841 tgttttgttt tgatagactt agctcatgta ccggtcatgg gcattcggcg tcgttctcgt
      901 ctgcgggacg gcgcacgccc agcaagccac caccgagatc tggcgctgca tcgatgccga
      961 cggccgccgg cactacacca acgccaagaa agagaccgcc ggcatgaagt gcgagctggt
     1021 gacgagccag atcaacgtcg cgccgccgct gtcgcagccg aaggcggggc gcacgtcgcc
     1081 gggatttccg cgcgagaccc cgcagcagag cgtgagcgcg cgcgagcgcc agcgcgagat
     1141 cctcgagggc gagctcgccg cggagcgcga atcgctgaac caggccaagc aggcgctcga
     1201 ggcgcaggag gcggtccgca gcggcgacga acgcaactac gcgcgtgtcc aggaacgact
     1261 gcaaccgttc aaggacgcga tccagaacca cgagaaaaac atccaagcgc tccagcgcga
     1321 gctcggcaac ctgaaataac aagtccgtgg ccgcctcgtt cgccggcctc gacctgctgg
     1381 ccacggcagt ggtggtgctc gacgatgagt tcgtggtgcg ctacgccaac ccagcggccg
     1441 aaaacctgct cactacgggt gcgcgcagcc tgatcggcca gcctttcctg ggctttttcg
     1501 ccgagcgtac cgagctcgag cgctcgctcg aggacgcgcg cgtcatccat tgggattact
     1561 ccgcgcgcaa cgtcacctac gtacgcgttg ggcgccagcc ggtgccgctt tcctgcaccg
     1621 tgacgcgcat cgactccttc ggcatggcgc tgctcgccga gctgcgcccc atcagcgagc
     1681 aactccggat cgagcgtgag gcgcgcatgg agtcggagca gcaggcgaac cgcgagctga
     1741 tccgcaacct tgcgcacgag atcaagaacc cgctcggcgg gctgcgcggc tcggcgcagc
     1801 tcctcgagcg cgagctcgag cgccctgagc tgcgcgaata cacccaggtg atcatcaaag
     1861 aagccgaccg gctgcagacg ctgatggacc gcatgctcac gccgcatcgc gcgccgcgcc
     1921 tcgagcccct cggcctgcac gaggtgctcg agcgcgtaag gagcctggtg cgcgccgagt
     1981 tcggcatcga gatcgtgcgc gactacgacc cgagcctgcc cgacatcctc ggcgaccgcg
     2041 agcagctcat ccaggcggtg ctcaacatcg cgcgcaacgc ggcgcaggtc ggaagcggca
     2101 gcgtcacctt caggacgcgc gtgctgcggc agatcacgat cctgaagaag cgctacaagc
     2161 tggcattaga attgcaagtc atcgacgatg gcccgggcgt gccggaggag atccaggaca
     2221 ggatcttcaa tccgctggtc tcgggcaggg aaggcggcac cgggttgggg ctctcgctcg
     2281 cgcagacgtt cgtgcactac caccacggcg tcatcgagtt cgagtcgcgg ccgggccgaa
     2341 ccatattcag gattctgctg ccgatcagat gaaaccggtc tggatcgtcg acgacgaccg
     2401 ctccatccgc tgggtgatcg agaaagccct cagccgggag ggcatcgcgt acaactcgtt
     2461 ctcctcggcg caggaggcgc tcgatgcgct ctcggtcgat gcgcccgagg ttctcgtgtc
     2521 cgacatccgc atgccggggc gctcggggct cgagttgctg caggccgtca agcaacgcca
     2581 tcccgcggtg ccggtgatcg tcatgacggc ttactccgat ctcgacagcg ccgtggccgc
     2641 gttccagggc ggagcctacg agtacctgcc caagcccttc gacatcgacc aggcggtcga
     2701 tctcatccgg cgcgcccttg acgagagaaa gagggaatcc gaggcggcgg agccgcccgc
     2761 cgcgacgccg gaaatcctcg gccaggcgcc ggcgatgcag gaagtgttcc gcgccatcgg
     2821 caggctctcg cagtcgagcg ccacggtcct catcaccggc gaatccggaa ccggaaaaga
     2881 gctcgtcgcg cgcgccctgc accggcacag tgcacgcgcg cggcaaccct tcgtcgcgat
     2941 caacaccgcg gcgatgccga aggacctcct cgagtccgag ctcttcggcc acgagcgcgg
     3001 ctcgttcacc ggcgcgcagc aacagcgccg cggccgcttc gagcaggccg agggcggcac
     3061 gctgttcctc gacgagatcg gcgacatgcc ctcggagctg cagacgcggc tattgcgcgt
     3121 cctgtcggat ggcacgttct atcgcgtcgg cggccaccag cagatcaagg taaacgtccg
     3181 ggtgatcgcg gccacgcacc agaacctcga gcagcgcgtt cgcgagggca ctttccgcga
     3241 ggatctctac caccggctga acgtcatccg cctgcgcctg ccgagcctgc gcgagcgctc
     3301 ggaggacatc ccgcttctgg cgaagcattt cctcgcttcc agcgcgaagc agcttggcgt
     3361 cgagcccaag cgcatcgccg acgatgcgct cgcgcacctc gcgcgcctcg aatttcccgg
     3421 caacgtgcgc cagctcgaaa atctgtgcca ctggctcacc gtgatggcgc ccggccaggt
     3481 gatcgacctc ggcaacctgc cgcccgaatt ccgcgctcag gttcctggcg aaagcgtgac
     3541 cgcggcctcc gactggctag ccgcgctcga gcaggaagcc gagcgccgcc tcgcgcgcgg
     3601 cgacactggc atcctcgacg cgctcggacg tcagttcgag cgcgccctta tcgtcaaggc
     3661 cctcgcgcgc accggcggac gtcgcatcga cgccgcgaat ctactgggaa tgggccggaa
     3721 tacgattacg agaaagattc aggaactggg gatagaagac aagggcgggc aggacgaaac
     3781 ttgaggtcgc aaacctaatt cggggtcagg gtcgcgactc gtcttacgta ttcggaccct
     3841 gaccccgaat tctgctacgc ggcttttttc cgattggctt cgagcttggc gtcgacgaga
     3901 ccgaccacct catcgagcac cggcaactgc aggcctttct gctttgcgat cgcctggaca
     3961 agccggtcga ctcgctcgat atacggcgcc ccggcataga gagcgcgcgc ggctgacgaa
     4021 gggctgccga ggctctgcgc cgcggccgca tatttgtcga acggcacgag gtcgctttcc
     4081 ttcgcgccga gcagaaggca cagattcacc acccattggt acgtggcgcg cgaagcgttg
     4141 atgtcggagt gcacggcgtc cttgatggcg cgcgggcctt ccctggtgat gcagcggtag
     4201 ttgcccgcga tcagcatcgc ccacttggcg agcggcacga ataccgactc atgcaccttc
     4261 agcttcaccg gcagctccat cttgtcgaaa cgagccgctt cgatgtccgc ttcgagctgc
     4321 ttgaggatcg cggtgtgcgc gtcgccggga aagcgcgccg acttgaagtt ggtcggcagt
     4381 cggacctgca gcacgttgat cttctcttc
//
LOCUS       DATBSZ010000273        64843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1350589, whole genome shotgun sequence.
ACCESSION   DATBSZ010000273 DATBSZ010000000
VERSION     DATBSZ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 64843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64843
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350589"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VIG70_17625"
     CDS             complement(<1..498)
                     /locus_tag="VIG70_17625"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8252415.1"
                     /translation="MIRSLALALIALALPAHAQAPLTFSSWVPPTHHLTIWQANWAAD
                     VEKATGGRVKFQSLPKHPAAAPGTFDAVRDGLVDLSYVTASYTPARHILPLMAELPGA
                     GETAEINSVAFSRIHWKHFQKVGEYKGVKLLAVWTHGPGQMFTKKQIKSLADFQNQKI
                     RTGGGI"
     gene            650..937
                     /locus_tag="VIG70_17630"
     CDS             650..937
                     /locus_tag="VIG70_17630"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY8252416.1"
                     /translation="MPHVVTKSDLVQVAAKAGNVSKVAANEAVNAVFEAIVSNVAKGK
                     RVTVVGFGTFLPRTRKARAGRSPVNGKEIKISAKTIPAFAAGQGFKEAVGK"
     gene            1036..1614
                     /locus_tag="VIG70_17635"
     CDS             1036..1614
                     /locus_tag="VIG70_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY8252417.1"
                     /translation="MDKPLILSLDAHGVPHRWISWQQACFYYAKDLVAWTLGEEKFTY
                     YGGICRRTGERSSITAHSIVAIKGKALAAKGFNQVPPLNNRELFRRDRHMCAYCGGEF
                     SYFRLTRDHITPLSRGGRDTWMNVVTTCRHCNGHKRNRLPQECGLELLYAPYVPNKAE
                     YLILTNRRILADQMEFLKQHVASHSRLVLAPA"
     gene            1620..2624
                     /locus_tag="VIG70_17640"
     CDS             1620..2624
                     /locus_tag="VIG70_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HEY8252418.1"
                     /translation="MPEFWRNSGFHLLKRDPAGRLTVTDDFLRAYYLRPEIHPVEESG
                     DAELRLHSSLMNAPRRSVGEKELEAIEDADARDNYRVILKFRQKLLDAGTVEGCYRGL
                     FKTGVDVPPMFVDQLVHVILRNVLDGCDDPLKLRAAELFFREQKATIQEGHALLADLE
                     TVEMHASGNRYGAIGKLIVEAQGDLGKVDLDVLDRANAALYWERDSRHDTVISLTYGR
                     PALDALCRVMEAWVSHFLKTKVQVKPIRKIDEAPWAWHVGLDAESTAILNELWSGAQI
                     EQGRMRNILAIFGLQFDDPAAMRTDLRGRTIYLALSCEDGVVRMKPQNLLTNLPLHEA
                     "
     gene            2614..3234
                     /locus_tag="VIG70_17645"
     CDS             2614..3234
                     /locus_tag="VIG70_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY8252419.1"
                     /translation="MKPDYWDRAKRALSRNDAVMKEIMRSRRGVHLVRRGEPFLTLAR
                     AICGQQISVKAAQSVWDRVCICLDQKITPEAVIAKDRKVLRACGLSDRKTEYIADLAQ
                     HFADGSIHVHRWPQMPDEEIIAELVEVRGIGRWTAEMFLIFNLLRPDVLPLDDLGLQK
                     AIRLLYFRKRPVSLRTMRKLGESWRPWRSVATWYLWRSLDPVPVEY"
     gene            3240..4208
                     /locus_tag="VIG70_17650"
     CDS             3240..4208
                     /locus_tag="VIG70_17650"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY8252420.1"
                     /translation="MKTTFLEFEQPIAELETRIEELRFVQDDSAVDISEEIQRLTKRS
                     QSLTKDIYGKLSPWQVAQVARHPQRPFTLDYVQALFTHFEELHGDRTFSDDASIVGGL
                     ARFNGTACMVIGHQKGRDTKEKILRNFGMPKPEGYRKALRLMKLAEKFALPVFTFVDT
                     PGAFPGIDAEERGQSEAIGRNLFEMARLRSPIIVTIIGEGGSGGALAIAVGDIMLMMQ
                     YATYSVISPEGCASILWRSADMAPDAADALGITASRLKTLGLIDKIVPEPLGGAHRDP
                     QVAAQALKKALAEALKQLQEKKPKELVETRLERLMAHGKFKEAAER"
     gene            4177..5349
                     /gene="tilS"
                     /locus_tag="VIG70_17655"
     CDS             4177..5349
                     /gene="tilS"
                     /locus_tag="VIG70_17655"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY8252421.1"
                     /translation="MASSRKRPSGRGIAVALSGGVDSVVLLHRLRNEPGLRAIHVHHG
                     LSPNADAWAAFCRRLCKRLGVPLVVKKVKVVKQGEGLEAAARAARYEVFRKIPAEAVV
                     LAHHLDDQAETVLMNLLRGAGRRGASGMPRESRFHGKRLLRPLLDVPREAIVAYAREH
                     GLTWIEDESNADESLTRNFVRRRLAPLMQQKYPKWRENLARAARHFSRQEAGAQDLLR
                     KFLQAKGLRAPSEAKLVEMLKQLTSQGSKTLIEHDGARLRVYRGKIMEDRGAGSFSPI
                     PWKGEPKLPIPELGGELRFRAVRGKGFSKNLFKKTLSIRLRAGGERLQPDPRRPRRTL
                     KNLFQEAGVPPWERDRLPLLTSGDDVVWVPGLGVDVKYRAAKNASGILPEWRKMPG"
     gene            5380..5814
                     /gene="ndk"
                     /locus_tag="VIG70_17660"
     CDS             5380..5814
                     /gene="ndk"
                     /locus_tag="VIG70_17660"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY8252422.1"
                     /translation="MAVEQTLSIIKPDAVKKNVIGKILARFEAAGLRIVAARMAHLSR
                     KEAEGFYAVHRERPFFKDLVEFMISGPVMIQVLEGESAIARNRELMGATDPKKAAKGT
                     IRADFADSIDANAVHGSDGPETARKEIAYFFPKVDERKPARA"
     gene            5789..6871
                     /gene="rlmN"
                     /locus_tag="VIG70_17665"
     CDS             5789..6871
                     /gene="rlmN"
                     /locus_tag="VIG70_17665"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY8252423.1"
                     /translation="MRENLLGLDAAGLERFFEAQGEKPFRARQLLRWIHQRGEGDFAG
                     MSDLARALRERLPSIAGVEAPVIVGDTAAADGTRKWLLKVDRANAVEAVFIPETRRGT
                     LCVSSQAGCVLDCAFCSTGKQGFNRNLSTAEIVGQLWLANRLLKGDNHGERPVTNVVM
                     MGMGEPLLNLDNVIPALRLMLDDNAYGLSRRRVTVSTAGVIPGIERLRDECPVALAVS
                     LHAPNDELRDRLVPVNRKYPLAELVKACKRYLDKAPRDFITFEYVMLEGVNDGDAHAR
                     ELVALAAKVRSKFNLIPFNPFANSGFKRSSPERIRSFAEILQRAGLTVTIRKTRGDDI
                     AAACGQLAGDVTDRTRRSLALKELRA"
     gene            6868..7653
                     /gene="pilW"
                     /locus_tag="VIG70_17670"
     CDS             6868..7653
                     /gene="pilW"
                     /locus_tag="VIG70_17670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEY8252424.1"
                     /translation="MKRLLLVMLLAGCAAKDTGPSQESVSIIGEAADPRNRAKLHTDL
                     ASLYFGAGNFGVALEELRLAQSADSNYAPTHGMFGLLYMELKENARAEESFERALRLA
                     PNDSDINHNYGWFLCQTGREPASIKYFLVAVRNPLYTTPWRSYSAAGVCTLKLDESKS
                     KSKEKDAEAFFERALRLEPDEPASLLQLGQIRYRQGNIADARKLVARYNKVANPSAES
                     LWLAVRIERRLGERLQEQAYANQLRRRYPASAENQSLQRGRYD"
     gene            7670..8521
                     /locus_tag="VIG70_17675"
     CDS             7670..8521
                     /locus_tag="VIG70_17675"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEY8252425.1"
                     /translation="MRTGVGQELAAAREARGLALSDVAQQLKFAPRQLEALEQEHLDE
                     LPGATFARGMVRSYARLLKLDPEPLVQRIAGRFEVPDSNRLAARYHQPVPFSDGARRS
                     TFVYLAMSLGVLVLVGAVAYEWHQERTAAARSTTLGATSVAKATPAPREEKPHAAPIA
                     PVPVQQATAPAPAATEKTPKTVEKPPKTIEKAPLAAVPATASGTGANRLVVRTEGEAW
                     IEIRDSADRMLISSLNPPGSERVVRGKPPYSLVIGNASQVSITYDNKPVDLTPHTRQD
                     VARLTVP"
     gene            8527..9756
                     /gene="ispG"
                     /locus_tag="VIG70_17680"
                     /gene_synonym="gcpE"
     CDS             8527..9756
                     /gene="ispG"
                     /locus_tag="VIG70_17680"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY8252426.1"
                     /translation="MQRRTSRQVAVGGVRIGGGAPIVVQSMTNTDTEDAGATARQVEA
                     LARAGSELVRITVNTPKAAIQVPRIRELLDSVGCAVPLIGDFHYNGHKLLTQYPDCAR
                     ALAKYRINPGNVGKGAKRDEQFATLIECALRYDKPVRIGVNWGSLDQVLLAQLMDENA
                     KKSIPSDASEVMKEAVIRSALESAGQAEAWGLPAERIVISCKMSQVQDLIAVYRALAA
                     RCEYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGGDRAREVQVA
                     QEILQTMGLRAFTPMVSACPGCGRTTSTVFQELAKNIEGFLREQMPVWKSQYPGVEEM
                     RVAVMGCVVNGPGESKHADIGISLPGTGEVPVAPVYVDGKKTVTLKGERIAEEFQAIV
                     EDYVRKKYGKRIPIKAI"
     gene            9758..11113
                     /gene="hisS"
                     /locus_tag="VIG70_17685"
     CDS             9758..11113
                     /gene="hisS"
                     /locus_tag="VIG70_17685"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY8252427.1"
                     /translation="MQKVKPALLRGFDQEYLPHDQLHFNRLIDIIRRNFELYGFLPIE
                     TPSAERREVLTSKGGVEKEIYALTRLAEADDDEAATKGALRFDLTVPLARYVAMRERE
                     LAFPFRRYQIQRVWRGETPQARKGRFREFYQCDIDIINRDKLSYLAEAEIPSVIYSVF
                     REMAIGEFRIRINNRKVLKGLLQSFDIPERSSGAVLRTLDKIEKEEAAKIRADLEREG
                     LSRDAAGELLDMISTRRDTKATLEALARIKNETAAQGLDELRKIVEAIGQFGVPDSAF
                     AVDLGVVRGLDYYTGTIYETTLAGAPELGSICSGGRYDDLASYFTDTKLPGVGISIGL
                     TRLFSKLKEAGLLQPLPRSPAEVLVTTMDPRYLDKYFALGRVLREAGINTEVYLETAK
                     LGNQLTYADRKGFRVAIIAGENEFAKDAVQIKDLRAKTARDVALAEIVPSVQATLGGS
                     D"
     gene            11204..11299
                     /locus_tag="VIG70_17690"
     CDS             11204..11299
                     /locus_tag="VIG70_17690"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252428.1"
                     /translation="MVVAHAEHNDTIRIISARLATAGERTIYEEG"
     gene            11286..11546
                     /locus_tag="VIG70_17695"
     CDS             11286..11546
                     /locus_tag="VIG70_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252429.1"
                     /translation="MKKASGKSDEALRPEYKRSDFGTLVRGKYARRVAEATNVVVLEP
                     QVAQAFPNDYAVNKALKGLLRGRRQSARPTARARTRRERRTD"
     gene            11564..12196
                     /locus_tag="VIG70_17700"
     CDS             11564..12196
                     /locus_tag="VIG70_17700"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8252430.1"
                     /translation="MPLDLEEQEQVAELKAWWRQHGNLIVSAVLAAALAFAGWQGWRW
                     YSASQAAHAAAQFEALAKAAQAGDAKALRDAAGTLIESYPRTLYASMGALVAARFHFD
                     RNDLKAAKTQLQWVVESAPSEDFRDLARLRLAAILLDEKAHDEALKALDARHSAAYDS
                     QFAALRGDVLVAKSQPAEARAAYQLALDKAGKEQNAFRESVRMRLEALGG"
     gene            12199..13317
                     /gene="bamB"
                     /locus_tag="VIG70_17705"
     CDS             12199..13317
                     /gene="bamB"
                     /locus_tag="VIG70_17705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEY8252431.1"
                     /translation="MKWKAGMVMTGIVFAGCASDPGPKPAEPPRIEKAQRVRTLWSAS
                     VGGGQGFTFQPALAAGSVYAAARDGTVMRFDEKTGDTRWRAELELKLSGGVGADELTV
                     AVASEEGEVVALDAQTGKVRWRARVSSEVLAAPAIGAGLVLVRSIDNRVFAFGADDGK
                     RRWVYQRAPSALLVRAPAGITIDGGLAFAGFPGGKLAALALESGAVRWEATVAVPKGT
                     TELERVTDVIGSPAAQGREVCAAAYRSRLACYDVATGRQVWARELTSLSGVSLDARYA
                     FVADDRGTVHALDRSNGQSVWKQDKLAYRQLSTPLAGGESIAVGDFEGYIHFLARDTG
                     VFVARGETSGRAVRATPLRLGAGLLVQTEDGGLFALSL"
     gene            13314..14654
                     /gene="der"
                     /locus_tag="VIG70_17710"
     CDS             13314..14654
                     /gene="der"
                     /locus_tag="VIG70_17710"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY8252432.1"
                     /translation="MKPVIALVGRPNVGKSTLFNRLTRSRDAIVHDVPGVTRDRHYGE
                     GRLGSRAFIAIDTGGFEPRSPQGIFVQMARQAEQAMVEADAIVFLVDGRAGLTPSDRE
                     IAQKLRKLKTPAFVAVNKTEGMQSDIAVAEFHELGLGAPAAISGAHGEGVRELMDLVL
                     EKFPEDTEPDEEEKGHPRVAVVGRPNVGKSTLVNALVGEERVIAFDQPGTTRDPIEVP
                     FERGGRRYTLIDTAGLRRRGKTGEPVEFFSIVKALQAIEAANVAILLLDASTGITEQD
                     AHVAGYVLERGRAVVLAVNKWDAADKELRERMKQELAWKLEFLGFAETHFVSATKGKG
                     LGPLMRSVDAAHGAAMARLATPKLTRAMIAAVERQSPPKTGRVRPKLRYAHQGGINPP
                     RIIIHGNALDRVPETYKRYLENYFRNAFKLAGTPLRIEFRTGRNPYVKSRAARG"
     gene            complement(14629..15363)
                     /locus_tag="VIG70_17715"
     CDS             complement(14629..15363)
                     /locus_tag="VIG70_17715"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8252433.1"
                     /translation="MKLVAVIPAYNEAPTIREVATRTLRLVPDVIVVNDGSTDATAAA
                     LDGLRVTVISNPVNYGKGASLWRGFALALADGADAVVTLDGDAQHDPEDIPRLIDAAR
                     AHPDRIIIGARLWDREKVPPLRYLGNRFANFWVAWAAGFPVADSQSGFRLYPASVLRE
                     IQLFHGPNARFAFESEILIEAGRAGVRSAAVPIAALYPPDARPSHYRSTVDTARIARM
                     IFWKLLSRGFDLPALVRSLILARRGT"
     gene            complement(15360..16076)
                     /gene="fabG"
                     /locus_tag="VIG70_17720"
     CDS             complement(15360..16076)
                     /gene="fabG"
                     /locus_tag="VIG70_17720"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006755110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY8252434.1"
                     /translation="MRALVTGGSGTIGAAICRRLAAAGRHVAVHANRNLAEAERVAQN
                     IRAAGGAADAISFDVTDEKATAAALEHLLADGPVQIVVNNAGVHADAPMAGMSGEQWR
                     SVVDVSLHGFFNVTRPLLLPMIGTRWGRIVSLSSVAALRGNRGQANYAAAKSGLHGAT
                     RSLALELASRGVTVNAVAPGIIDSPMTAQAFTKSAIDALVPMKRAGQPDEVAALVGFL
                     ASDEAGYISGQVISIDGGMT"
     gene            complement(16076..16504)
                     /locus_tag="VIG70_17725"
     CDS             complement(16076..16504)
                     /locus_tag="VIG70_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013207021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HEY8252435.1"
                     /translation="MPVSKAEIAGMIPHAGRMCLLHEVLEWSEAAIRCTSRSHRDGDN
                     PLRAGAELPALCGIEYAAQAMAVHGRLSAAVRETPHAGYLASVSDVVCSAARLDDLEH
                     ELVIDAERLAGDGERALYRFALSCGGVEILRGKAAVVLSG"
     gene            complement(16495..17292)
                     /locus_tag="VIG70_17730"
     CDS             complement(16495..17292)
                     /locus_tag="VIG70_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEY8252436.1"
                     /translation="MRAFIEGIGVLGPGLSGWPAARAVLAGAEPYRPARTAIPPSELL
                     PPAERRRAGIPVKLALAVGREAFAAAGHDAAVTPTVFTSSSSDGEILHEMCETLATAE
                     REVSPTKFMNSVHNAAAGYWSIATRSREPSTSLCCFDASFAAGLLECAAQVAVDRRPV
                     ALVSHDQPYPEPLHAARPIASVFGVALILVPEETRHSVAAIDLELRPADGAETRLADP
                     ALEAARQGNPAARSLALLAVLARGVAGDVVLNYVAGTQLRARVTPCP"
     gene            complement(17276..18475)
                     /locus_tag="VIG70_17735"
     CDS             complement(17276..18475)
                     /locus_tag="VIG70_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY8252437.1"
                     /translation="MQPLLITRFSIVNCLGTGSAAVLDALRTKRSGLTPCEFETTSID
                     TWVGSVPGLKDAGIRRGLEAYDCRSNRIAQMALEADGFAAAVAAARGRYGASRIGVFI
                     GTSTSGILEAELAYQRRDPASGSLPRGFRYHATANTYSLGDFVGRYLGLAGPSFVVSS
                     ACSSTAKVFGNAARMIAAGACDAAVVGGADSLCLTTLYGFRSLELTSRRPCRPFDRER
                     DGISLGEGAGFALLEKPHAGASADAPILLGVGESSDAYHMSAPHPEGAGAKLAMQRAL
                     ASARLGAADIDYINLHGTATPSNDAAEDTAVVDLFGRKTPASSTKGATGHLLGAAGIT
                     EAIISTLALRHGLLPGSAHTRNVDPALKSNYVLETREARLRRVLTNSFGFGGSNCSLV
                     IGDARVH"
     gene            complement(18460..19224)
                     /locus_tag="VIG70_17740"
     CDS             complement(18460..19224)
                     /locus_tag="VIG70_17740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY8252438.1"
                     /translation="MTPVIRVSILVHAAGVIVLALAPGMWPWVGAALIANHLVIAAGV
                     FHVRGRLLGPNLSRLPAAAAAHRHVCLTFDDGPHPELTPRVLELLERYQAKASFFCVG
                     ERARAHPEIVREIVRRGHSVENHSDRHSHAFAFYGISLLRREVERAQATVAAITGQAP
                     QFFRAPAGFRSPFLGLVLPPMGLRYVSWTRRGLDTVARDPARVLGRLTAGLAAGDVLL
                     LHDSEPLSLAVLPALLEQLAARGLKCVSLPAACSLS"
     gene            complement(19221..19790)
                     /locus_tag="VIG70_17745"
     CDS             complement(19221..19790)
                     /locus_tag="VIG70_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252439.1"
                     /translation="MRALHDRAPGASGNLLVMLPPAKACAQDLVDRGFVAALRERGSP
                     LDVAVMDADADYYLEGSVGERLAADVIAPMRANGYARIWLMGISLGGYGCISLARRRA
                     AELEAMILLAPFLGSRGPDALEPADAKWFPAAYLGFGTGDRYAAASEMLARQLPAERV
                     IRMPGAHDWPTWIQLWRAVLEKPLFPKPA"
     gene            complement(19774..22107)
                     /locus_tag="VIG70_17750"
     CDS             complement(19774..22107)
                     /locus_tag="VIG70_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY8252440.1"
                     /translation="MSPAARWALAAWIAGAAACAAVIARTEFSTDLSAFLPRSPSPVQ
                     QALVEQLRDGVVSRLTLIGVQGDSPDRLAEVSKRLAASLRASREFAAVNNGEEAGFEK
                     DRDLVWRHRYLLSPAVKAERFSAAGLRASLEELLAQLASPAGVLLQRLSASDPGGELL
                     RLLEELAPATRPRLHGGVWFSEDSRRALLVAQTRAAASDIDAQKRALGLIQDSFAAAA
                     AGAGARLQVTGPGVFSVASRDKIRADAWRFSLIATVLIAAMLIALYRSVRVLGLGLLP
                     VASGALAGVAAVSLAFGSVHGITLAFGVTLIGEAVDYAIYLFTQNAPGATAQSTFERI
                     WPTLRLGLATSICGFSAMLFSGFTGLAQLGLFSIAGLVVAAAVTRWALPAWLPRGFAV
                     AAVAGFAPRVMALARAAPRLRYPLVVLIAAAAAFLLLRPQPLWSGSAASLSPISPREQ
                     ALDRELRQDLGAPDPRYLVVVHAADEQAALQAAERAALALRDAVAKGWLQGFDSPAAY
                     LPSEAAQRERQAALPPPPTLRQNLQEAQKGLAFRPNVFEPFVRDVGAARTMPLLDRAS
                     LQGTSLALRLDALLVKRPTGWAAMLPLRGVSDAKAIAGAVAGTSAGVLLDLRQELDEL
                     YRGYLREAQINSLLGAAAIVALLLAALRSPRRVFDVLAPLAAAALVTCGLLALGGHEL
                     SIFHLVGLLLVVAVGSNYSLFFDRAAPVQDRGRTILSLVFAAVTTVIGFGLLAFSSVP
                     VLNAIGSTVGLGAAMALVFSAILSRPSGAEGAYARAS"
     gene            complement(22104..22673)
                     /locus_tag="VIG70_17755"
     CDS             complement(22104..22673)
                     /locus_tag="VIG70_17755"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY8252441.1"
                     /translation="MVLALGCLAPALAQAQDKPTLVLERLMKDLAQVKSARGRFVERK
                     HFAMLNAPLESSGTLVYVAPGRLEKHMTSPRQESLILEGDRLIVERKDKGQRRTFPLQ
                     EHPVLWAFVESMRSTLNGDLATLQRFYEVRLEGDAGAWRLRLRPKEPRMRQAASEIRV
                     GGSGTWVNRIEIDEAGGDRSVTEVTREAG"
     gene            complement(22684..23598)
                     /locus_tag="VIG70_17760"
     CDS             complement(22684..23598)
                     /locus_tag="VIG70_17760"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEY8252442.1"
                     /translation="MLRQPGRVAAPPDWVEHPERGSVLAMRLAVWLMLALGRPLGRIL
                     LVPICAYFVLFSSHARSASAGYLRRVLGRRATLGDVFRHYYTFASTIQDRVYFLAGRI
                     SSYDVQIHAGLQTQALLRDGRGCILLGSHLGSFEVLRACGPGEGVPPINILMYVDNAG
                     KLNSVLRPLAPGAEARVIPLGSPDALLRVHECLARGEIVGILGDRAWRSERTYRCEFL
                     GGEARFPLGPLLLAGLVQAPVVLFFGIYRGGRRYEIHLETFADAIPLERRNREESVRP
                     WVERYAKRLEHHCREAPYNWFNFYDYWA"
     gene            complement(23574..23879)
                     /locus_tag="VIG70_17765"
     CDS             complement(23574..23879)
                     /locus_tag="VIG70_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252443.1"
                     /translation="MATIRQRLSAEHPAAQGHFPGNPVIPGAVLLSEAVRLIEADLGV
                     SLAPYRIRRAKFPSPARPGDEVLIEFSDGLGSIKFECAVKGNTVLVGEVECSASPAE"
     gene            complement(23881..25173)
                     /locus_tag="VIG70_17770"
     CDS             complement(23881..25173)
                     /locus_tag="VIG70_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8252444.1"
                     /translation="MAAQPLVRHDPDALFAQRQGELVRAGEFLAEVARLAEALPDRRY
                     VINLCTDRFRFTAGFAAALVRGQISLMPPTHTPDVIERLVRDYPDLYCLDDDFAAADG
                     GRGVPSPAVPAIPEERIAAIVFTSGSTGDPVPHPKSWGALVATAIAEIERLRIRDFPG
                     MAILGTVPPQHLYGLESTVLLPLQGALVLHGGRPFFPADICAELEALPRPRGLVTTPV
                     HLRALLADATALPSLDFLLSSTAPLPTPLAVEAEARLGAPLHEIYGCTEAGLVATRRP
                     SESAEWQLFPHLTMRQDEKGTWVKGGQAPMEFLLSDVIELQTRGRFLLHGRTADLVNV
                     AGKRTSLASLNHHLTSIPGVRDGAFVVPADDPGGVTRLAAFAVAPGLTAETILAALRQ
                     RIDPAFLPRPLCIVESLPRTETGKLPRAELDSLVAKVG"
     gene            complement(25184..25849)
                     /locus_tag="VIG70_17775"
     CDS             complement(25184..25849)
                     /locus_tag="VIG70_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252445.1"
                     /translation="MARPAAIAWKVLAAAAIVGYPILIHVGLAGSAGDPARTLLVMAP
                     MAALGCWLIARSQKRMLWLLIVAGAAGATWLLAHGTGLGTTVLYGVPHAAAYSFMLWL
                     FGRTLLPGREAIITRLARLARQAHGATLPPAMERYTRRLTAAWCVFFAAQLAGSALLL
                     AYGSLESWSLFINVLSFPLVGLMFALDYLYRVIRYRHLPQSSIATAIQAYAKDRASSL
                     LSR"
     gene            complement(25851..26150)
                     /locus_tag="VIG70_17780"
     CDS             complement(25851..26150)
                     /locus_tag="VIG70_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY8252446.1"
                     /translation="MSIVTTEIAPQTDALTAEELELARVIVATLKLEMEPAEVSPTAP
                     LYGDGLGLDSIDVLEIALAISKAYGVKLRSDNKNNASIFSSLRSLSQHIQAHRAP"
     gene            26251..27534
                     /locus_tag="VIG70_17785"
     CDS             26251..27534
                     /locus_tag="VIG70_17785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8252447.1"
                     /translation="MVNHRCDVLVIGAGPAGSTVAALLAERGREVVILEKERHPRFHI
                     GESLLPLNLPLFERLGVAAEIQRIGMPKLGAEFVSPWHKQPVTFDFAHALDKSFPSAY
                     QVRRSEFDEILFKNAVRKGARALEACRVTTVEFTPQGAAVAARLEDGAEQRWDTRFVV
                     DASGRDTFLASKMGIKRRNRKHNSAAIYGHFTGAKRLPGKAEGNLTVFWFEHGWQWFI
                     PLADGTTSVGAVCWPYYMKTRKTGTDQFFLDTIALCPALAERLRDAQLTAPVTATGNY
                     SYVAERTMGRNYVMLGDAYTFIDPVFSTGVLLAMQSAFAGADTIETCLDHPAKAGAAL
                     RRFDAMMRRGPGVFSWLIYRVTTPALRELFMKPSNRFRVQESLISVLAGDIYRDTPVR
                     ARMLLFRAIYYLKSLSLLRQSVSAWLRRRRAIQSA"
     gene            27588..28127
                     /locus_tag="VIG70_17790"
     CDS             27588..28127
                     /locus_tag="VIG70_17790"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8252448.1"
                     /translation="MATYHHGDLRTAVLAAAGKLIEKEGLGGLSVREAARRAGVSHNA
                     PYRHFPDRDALLAALAAQGFSQLGKALEGRSGRELGEAYVRFALGHPQRFRLMFGGQL
                     ALDRYPELRTQAEATHGRLTAAFAGLGAEAKFAAAAAWSLVHGLAQLILDGHFENDEG
                     FVTKVIGAIRFAVGPQRSA"
     gene            complement(28112..31069)
                     /locus_tag="VIG70_17795"
     CDS             complement(28112..31069)
                     /locus_tag="VIG70_17795"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252449.1"
                     /translation="MSRGAFYVLFTASGFAGLIYESVWTHYLKLFLGHAAYAQSLVLA
                     VFMGGMAAGAAWCGRRSARLANPLAAYAIVEAAVGIAALVFHPVFVAVTDWSYDRLLP
                     GLGNEWLALGAKLAVSALLILPQSVLLGMTFPLMSAGLVRAHRAAAGESVAMLYFTNS
                     LGAAAGVLASGFVLIAWAGLPGTLRTAGVLNLVIAGVVWLLARPARHEPLKRVSVSES
                     NLGLLLAIAFFTGLASFVYEIGWIRMLSLVLGASTHSFELMLATFILGLALGGLAVRR
                     RADAAADPVRLLAGVQVAMGLAALATLPVYDFTFSLMEVLLKGLARSETGYLLFNLSG
                     AAIAAMVMLPATFCAGMTLPLITAALLRRGAGEAAIGQVYAANTLGAIAGVVLAVHAG
                     LPLLGLKGTLIAGALVDVALGLVLLQFVKRTYIQVAVVCGVLFLAVGIGVELDVHKMT
                     AGVFRHGELQTSQDATILFNKDGKTATVHLVRYSDATSLRTNGKSDGSINLNPAGERG
                     TDEITMVLTAALPLALKPGTKSAAVIGIGTGLTTHTLLQSLDIERVETVEIESAMAEA
                     SRGFMPRNSGAFADPRGAILIDDAKSFFSTRNRRYDLIISEPSNPWVSGVSSLFTREF
                     YRRIRKHLNPGGLLVQWIQLYEMDASLVATVLGALGAEFPHYAVFAASDYDLLIMAGD
                     APVPLEAQAGVFEHPGVAKELWTVHVLTAGDLDARYLGSRATLEPLFASYGMPANSDY
                     APVLDLNAARHRFMDKSAADVVALLNAELPLLELIERDRSRRAINPLFKGAYAFERVE
                     NTRLAWYARDFLLRPRPPVPESVPRELQKDLELVKLRLIECRDPRELDIWLHSALRVA
                     KALNPYLAPSDLAPVWARMMGSACYSSLQEFQRRWIALFQAVGARNATRMAELAGPLI
                     AGEQPLIADAREYLLLVALTGYVASGDKGKALELWKTYGSGTRAARPAFRLLRCHAEL
                     ATCAEAFASYAER"
     gene            31093..32202
                     /locus_tag="VIG70_17800"
     CDS             31093..32202
                     /locus_tag="VIG70_17800"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8252450.1"
                     /translation="MGFRLSAFYFAFFAYAGVYVAYFPLYLAGRGLSAVEIAWVLALP
                     PLVRIVAPGAWGWLADRTGAHRAIVAFSSAVTALAFALLPFTENVALLIALMSVFSAG
                     ALPIVEAITLGSLAGQSGRYGPIRLWGSVGFIAVVLAGGVWLDAQPVSTLPAVLVLFA
                     LFTLAMALALPSIKPLGPSRAATFRVSPEVRALFAAGFCNAVAHGALYAFLSLHLEAL
                     GYSATTIGMLWTAGVLAEIVVFLYLPALFRRFALSSVLVASLLLGVLRFAAIGWGAGQ
                     AWIVLLAQLLHAATFGSFHAASVAAVQRVFPEGAQARGQTLFSGFTYGAGAAAGLVLA
                     GWAWAGGGAPLAFSVSAMAAAAGAFFAARLKRAGL"
     gene            32244..33071
                     /locus_tag="VIG70_17805"
     CDS             32244..33071
                     /locus_tag="VIG70_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY8252451.1"
                     /translation="MIRAIVVAAAAAAAVLSGCQTAETTQAGVVGVDRDQRFLVSADE
                     VNAGAQKAYAQMMAEAQKKGVLDKEPAQVQRVQAITKRLVAQAGAFRPDAAKWPWEVH
                     VISLDEVNAWCMPAGKMAMYTGLIQKLNATDDEIAAVMGHEISHALREHSREQISRQM
                     GTQMGIGLVGALFGIGELGQSVAGQVANVTLNLPNSRQAETEADRIGIELAARAGFDP
                     NAAVSLWEKMAKLGGSQPPKWLSTHPPHEDRINDLRNYAQKVMPLYTAAKSGAGAGK"
     gene            33087..34499
                     /gene="leuC"
                     /locus_tag="VIG70_17810"
     CDS             33087..34499
                     /gene="leuC"
                     /locus_tag="VIG70_17810"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY8252452.1"
                     /translation="MGKTLYDKLWESHVVHVEDDGTALLYIDRHLVHEVTSAQAYEGL
                     RGAGRKPWRIESVVATADHNTPTTDWNQGIAGVRDPMARLQIETLDANMKEFGAKVYF
                     PYLDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGCLAHGIGTSEVEHVLATQ
                     CLVTRKMKNMRVNVEGPLARGVTAKDVVLAIIGRIGIAGGTGHAVEFAGSTIRALSME
                     GRMTVCNMAIEAGARAGMVAVDETTLEYIKGRKFAPQGSDWERAAGYWRTLKSDPDAK
                     FDREVTLDAREIRPHVTWGTSPEMVVSVEDRVPDPDKEKDSLRREGMERALEYMGLKP
                     RTPITDIRIDKVFIGSCTNSRIEDLRAAAAVVKGKRIASNIRAALVVPGSGLVKQQAE
                     REGLDRIFRDAGFEWREPGCSMCLGMNPDRLDPGERCASTSNRNFEGRQGTGGRTHLV
                     SPAMAAAAAIAGHFVDIRKW"
     gene            34493..35086
                     /gene="leuD"
                     /locus_tag="VIG70_17815"
     CDS             34493..35086
                     /gene="leuD"
                     /locus_tag="VIG70_17815"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY8252453.1"
                     /translation="MVKFTVLNGVVMPLDRANVDTDQIIPKQYLASIKRTGYAQGLFA
                     AWRKDPQFVLNQPLYKGASILLARKNFGCGSSREHAPWALLDYGFRCVIAPSFADIFF
                     TNSFKNGLLPVILSEAEVDRLFHECAAFPGFRLVVDLEQQSVSTTNNEKSFRFEIDPF
                     RKYCLLNGLDEIGLTLRHADAIRAFEAQRKAQYPWYF"
     gene            35088..36140
                     /gene="leuB"
                     /locus_tag="VIG70_17820"
     CDS             35088..36140
                     /gene="leuB"
                     /locus_tag="VIG70_17820"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY8252454.1"
                     /translation="MKIAVLPGDGIGPEIIAQARKVLQKLDLSFEFAEAPVGGAGYEA
                     AGDPLPAATLQLARAADAVLFGAVGDPRYDKLERAKRPEQAILGLRKELGLFANLRPA
                     RVHPELAEASALKPEVVSGLDILIVRELTGDIYFGKPRGIRQVEGIREGFDTMHYNEG
                     EIRRIARVAFEAARKRNKRVCSVDKANVLETSQLWREVVQQEAKSYGDVELTHMYVDN
                     CAMQLVRNPKQFDVIVTGNLFGDILSDEASMLTGSIGMLPSASLDERGKGLYEPIHGT
                     APDIAGKGLANPLATILSAAMMLRYSLRQPQAAQRVEHAVTKVLKSGLRTADIHTPGA
                     RRVGTQEMGDAVVAAL"
     gene            36153..37289
                     /gene="asd"
                     /locus_tag="VIG70_17825"
     CDS             36153..37289
                     /gene="asd"
                     /locus_tag="VIG70_17825"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY8252455.1"
                     /translation="MRVGIVGWRGMVGSVLVQRMREERDFDHVEPVFFSTSQAGGKGP
                     EIGKATEAVKNATDLAELKKLPVIISCQGGDYTSEIYPKLRQQGWKGYWIDAASALRM
                     NDDAVIILDPLNMDLIKSAIKAGTKNYIGGNCTVSLMLMGMAGLFQRDEVEWMTSMTY
                     QAASGQGAAAMRDLVAQMATIADRSQQLLDDPASAILEIDRNVTESIRARDLPRKTIG
                     YPLAGSLLPWIDKDLGNGQSREEWKAQAEANKILGRGNGKNGAKAIPMDGVCVRVGAM
                     RCHSQAMTIKLRRPVPMDEVEGMLADAHDWVKVVPNRREETLAELTPAAVTGKLTVPV
                     GRLRKLPMGDDYLAAFTVGDQLLWGAAEPLRRMVRILLDAGVKQ"
     gene            complement(37419..37721)
                     /locus_tag="VIG70_17830"
     CDS             complement(37419..37721)
                     /locus_tag="VIG70_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252456.1"
                     /translation="MQLDEQLDERLVDRLQRGHAKDAPPSATLDRETHGVQHRARLYA
                     RGSKRLLPSDARGQAFFFAGPQRDDFDFGVKGLSERGKNMEPAQARCMRGERRCER"
     gene            37665..40031
                     /locus_tag="VIG70_17835"
     CDS             37665..40031
                     /locus_tag="VIG70_17835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY8252457.1"
                     /translation="MTTLQPVNEPFVELLVELQWATGRLVREYTFLLDPPEYKARDAM
                     AAAPKPAPAEVKPAPAEAKPAPAEPPKPVPPLVEAKPIEPTPAPPVIAAAPVPAAEAE
                     KPAPVAEPAKEEAAKPEAAKEEPTQPEAAKEEAAKPEAAKEELVKEPEGAAKTYEVAK
                     GDTLGKIAQDNLPPGISLNQMLIAIYRANQDAFIRENLNLVRAGRILNIPSADSIGSV
                     DADEANRLVRAHMAEFREYRSRLAAVPATAEAAPGPRETAGTIEPKPAAPAPAAPQDQ
                     VRLSKADPQKPSASGAAAARGDDLVARERALKEAQSRIGDLEKNVADLQKLLELKNQQ
                     LAELERKAGAKPAPAAPVPAPAPAAKAPEKPAPEAPKPVVEAPKPAPTPAPAAPAPAA
                     KPTPKPAPPPAPEPSLLDEFLDNPVALAGLGGVVLLLLLYAVFAWRKKKTAQAKFQDS
                     VIGAAAAGAAAGAGMTEPTLGAATTASTQPVVSTQPAAAAEEVDPIAEADVYMAYGRD
                     AQAEEILKEALQKDASRIPVHAKLLEIYAKRQDTKAFEQSALKLKNLTNGAGPEWDKA
                     AVLGKSLDPNNGLYAGATSAAAAFPAAAAAPAAPAPTLDFDIGGATQGVSASAPDLTL
                     ELAPKSADGGLDFDVSGSTEKKDEETVVAPRPQAKDAGLDFDLDLGSAAPESKPASAG
                     PAPAADPGLTFDLNLDLGTEKKTEPVSGAGPDLSTISLDLDTADSGAAAPAGTDPKWQ
                     EIATKLDLAKAYEEMGDKDGARELLNEVMKDGDGAQKGQAQQLLAKLG"
     gene            40050..40799
                     /gene="truA"
                     /locus_tag="VIG70_17840"
     CDS             40050..40799
                     /gene="truA"
                     /locus_tag="VIG70_17840"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY8252458.1"
                     /translation="MALGLEYDGSRFLGWQTQPGGSTVQDALEAALASIAGTQVSTTA
                     AGRTDRGVHARMQVAHFDTAVKRPESAWVRGVNALLPDAVAVLWSTQVADEFHARYSA
                     LSRTYRYELLNRPVRAALSAHYAGWFHLPLDVEKMREAAALLLGEHDFSAFRSSECQA
                     KTPVRNIHAFTIDQEGERIVFVVRANAFLHHMVRNLVGTLVYVGKGKHPPQWVKEVLR
                     SGDRAKAAPTFGPEGLYLERIEYQPKWGLPI"
     gene            40808..41413
                     /locus_tag="VIG70_17845"
     CDS             40808..41413
                     /locus_tag="VIG70_17845"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY8252459.1"
                     /translation="MKICGITRTADAAAAARAGADAIGLVFYPPSPRFLSIERAREIR
                     DALPPFVQTVALFVNADAAQVSQVIGRVHPALLQFHGDEAPDFCAQFGLPFVKACRVK
                     KGVSALEYLRPFSSAAAWLFDSHVPEYGGVGESFDWSLVPVTDKPVILSGGLSQANVA
                     AAIRRVKPWGVDVSSGVESAKGIKDAAKIAAFMAEVRNADV"
     gene            41406..42599
                     /gene="trpB"
                     /locus_tag="VIG70_17850"
     CDS             41406..42599
                     /gene="trpB"
                     /locus_tag="VIG70_17850"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY8252460.1"
                     /translation="MYDLPDARGHFGPYGGVFVAETLTAALDELRLAYERARKDPAFE
                     AEFRYELKHYVGRPSPVYFAKRLTEKCGGARIYIKREDLNHTGAHKINNTIGQALLAR
                     NMKKPRVIAETGAGMHGVATATVAARYGMECVVYMGSEDIKRQAANVHRMGVLGAKVV
                     PVESGSKTLKDALNEAMRDWVTNVESTFYIIGTVAGPHPYPMMVRDFQSVIGEESIAQ
                     MQELAGRQPDAVIACVGGGSNAMGIFYPYLKHEKTRLVGVEAAGEGLETGRHAASLCA
                     GKPGVLHGNRTYLLQDANGQITDTHSVSAGLDYPGVGPEHAWLKDSGRAEYVAIDDEE
                     ALKAFHELCRAEGIIPALESAHAVAQAMKMAPAMAKDAILLVNLSGRGDKDLHTVAER
                     SGKKL"
     gene            42599..43396
                     /gene="trpA"
                     /locus_tag="VIG70_17855"
     CDS             42599..43396
                     /gene="trpA"
                     /locus_tag="VIG70_17855"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY8252461.1"
                     /translation="MSRIQGRFQALARDKRKALIPYITAGDPHPSLTVPLMHALVEAG
                     ADILELGVPFSDPMADGPVVQRAGERALKHGVGLSDVLRLVAEFRKSDNATPIVLMGY
                     ANPIEAMGVEKFVAAAKAADIDGVIVVDYPPEECEQFAALAKKHGIDPVFLLAPTSTE
                     KRIQQVARVGSGYLYYVSLRGVTGAGHLDFSEVSSKIPAIRAATKLPIGVGFGIRDAE
                     SARRVAQSADAVVIGSRIIQEIEAGTADGAVARVKAFLKPIRQALDG"
     gene            43393..44265
                     /gene="accD"
                     /locus_tag="VIG70_17860"
     CDS             43393..44265
                     /gene="accD"
                     /locus_tag="VIG70_17860"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY8252462.1"
                     /translation="MSWLQKLLPPKIQRTGPGARKTVPEGLWTKCAACDAVLYSTDLE
                     KNLNVCPKCGHHERLGARPRLDALLDPDGRFEIGSEVLPLDPLKFKDSRRYSERLAEA
                     QEETAEGDALVVMQGSIKALGIVCAAFEFDFMGGSMGSVVGERFVRGVRVAIEQKMPF
                     VCVTASGGARMQEGALSLVQMAKTTAALTDLAERGLPFVSVLSDPTMGGVSASFAMIG
                     DVVIAEPKALIGFAGPRVIEQTVRQKLPEGFQRAEFLLEKGAIDMIVDRRQLRDKLHA
                     VLSLLQGLPAETPA"
     gene            44262..45797
                     /locus_tag="VIG70_17865"
     CDS             44262..45797
                     /locus_tag="VIG70_17865"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY8252463.1"
                     /translation="MTLLAEVVKTSSTVAATSSRLEKTRLIAECLRSLSPDEIEIALP
                     YLSGDMRQGKLALGYSSLKAAMGRPASVPSLSVMDVDRAFQDLKTVKGKGAASTRERR
                     LKELFGKATAEEQDFLLRLIVGELRQGALEGVMLEAVAAAAGLPAAEVRRAATFAGGI
                     APVACAALAGGAKALERFSIRLMQPVLPMLAQPAEDLAAALAQLGEALLEWKLDGARI
                     QVHKSGGEIRVFTRNLNDVTARVPEVVSELRMIDADLILDGEAIALRADGRPHPFQIT
                     MRRFGRKLEVEALRDELPLSVFFFDCLLRDGEALVDRGAAARRRVLEESLPKALLAPS
                     LTTGDLGMAEAFQADALARGHEGLMAKAPAAPYESGRRGAGWLKIKSAHTLDLVVLAA
                     EWGSGRRRGRLSNLHLGARDPKTGGFVMLGKTFKGLTDELLEWQTRELQARETGRDEW
                     TVHVRPELVVEIAFNDVQGSPHYPGGMALRFARVKGYRPDKRPEEADTIDTVRAIYQR
                     GLA"
     gene            45794..47035
                     /gene="folC"
                     /locus_tag="VIG70_17870"
     CDS             45794..47035
                     /gene="folC"
                     /locus_tag="VIG70_17870"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEY8252464.1"
                     /translation="MKSLDEWLAFIERQHPQPIALGLDRVREVLARMNIRLQCPVITV
                     GGTNGKGSTCAMLESILRAGRYRTGLYTSPHLVNYNERVRIAGVELEDTALCEAFAAV
                     ERARGGVPLTYFEFGTLAAFWVFAREKIETAVLEVGLGGRLDAVNVLDPDCAVLTSVG
                     IDHVDYLGPDRESIGREKAGIFRAGRPAVIAEPDPPQSVMRAAAEKLLLGKEFGFTRQ
                     ENQWTYWGPRGQRAGLAHPALRGSIQLRNAAAALCALDAIELAIAMQDVRRGLAEVTL
                     PARFQVLPGRPQVILDVAHNVEAAQTLADNLLSSGYSSESIAVCGMLRDKDIAGVLRA
                     LAPRITRWHFASLPGARGASAEELARNIDGAVEKFESPTQAFVAAKGRAEEGDKIVVF
                     GSFLTVGEVMAWLQKNKTSKR"
     gene            47002..47445
                     /locus_tag="VIG70_17875"
     CDS             47002..47445
                     /locus_tag="VIG70_17875"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY8252465.1"
                     /translation="MAAEKQDVETLKRRGRRRLVGAVALVLAAVIVLPMVFDPEPRGT
                     PSAVNVRIPGEDDTPFAPKLPPRKKADAAPKEEFVVQVGAFASPQGAIAKLESAKMPY
                     TTEAMQGNLTRVRAGPFATREAAEKALEQLKGLGFEPGAVAPKAG"
     gene            47442..47930
                     /locus_tag="VIG70_17880"
     CDS             47442..47930
                     /locus_tag="VIG70_17880"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY8252466.1"
                     /translation="MTWLDYGVIVVLLLSIAWGAWRGLVHEVLSLAGWIMAFLAANLL
                     AAPLSETFPANMRPEFRVVGAFLLVFVGTLVLVTLLTALVTKFIRVSVLQSLDRWLGA
                     LFGLMRGLVLVVAFAVIAGLTSFPATPIWKDSATGYSLAQTVIQLKPWLPPALASRLK
                     YN"
     gene            47961..49472
                     /gene="purF"
                     /locus_tag="VIG70_17885"
     CDS             47961..49472
                     /gene="purF"
                     /locus_tag="VIG70_17885"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY8252467.1"
                     /translation="MCGILGIVARSPVNQLLYDGLLLLQHRGQDAAGIVTSERNSFHM
                     YKAPGTVRDVFRTRNMRSLPGNVGIAHCRYPTAGSAFNAAEAQPFYVNSPFGIVLGHN
                     GNLTNSDQLKEEMFRADRRHINTGSDSEVLVNVLAHELEQASVKLRLDPQTIFTAVAN
                     VHRRVRGAYAVVAMIAGYGVLAFRDPFGIRPAVIGYNETNAGTEYMVASESVAVDALG
                     FRVLRDIGPGEAVFFDEDGRFHHQQCAPHASLNPCIFEYVYLARPDSLIDGASVYESR
                     LNMGEKLAEKIRRQYRHLQVDVVIPIPDSSRPSALQLAVGLGVPYREGFVKNRYIGRT
                     FIMPGQAVRTSSVRQKLNPMAMEFSGKNVLLVDDSIVRGTTSREIVQMARESGARKVF
                     FASAAPPVRFPNVYGIDMPTRAELIAAHRSEDEVAREIGADALVYQDLDALKDAVRRC
                     NPKLTSFETSCFDGIYITGDITTDYLRSIEIRRHAKRDSGDDEAPQLDLNLAA"
     regulatory      49480..49557
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            49587..50780
                     /locus_tag="VIG70_17890"
     CDS             49587..50780
                     /locus_tag="VIG70_17890"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEY8252468.1"
                     /translation="MAKDYELATKGVRAGTARTEFQEHSEALFLTSSFVFKNAKQAAA
                     RFAGEDAGMVYTRYTNPTVSMFQDRLAALEGAESCVGTASGMAAILATAMVHLRSGDH
                     VVCSNAVFGATIQLFNTIFSRFGIETSYVSPTRTDEWARAVKKNTRLFFLETPSNPLT
                     EISDIPALAKVAKGAGALLAVDNVFCTPALQRPLELGADLVIHSATKYLDGQGRVIAG
                     AVAGSKALVGDPMTTFLRTAGPTLSPFNAWVVLKGLETLELRMLAQSAAALELARWLE
                     SHPKVSRVHYPGLESHPQHQLAKRQQRGGGAVVSFEVEGGRAAAWRVIDSTRLMSLTA
                     NLGDVKTTIIHPASTTHGRISPEARAAAGITDGLIRVAVGLEAVSDLKEDLQRGLEAE
                     QPSFS"
     gene            50828..52354
                     /locus_tag="VIG70_17895"
     CDS             50828..52354
                     /locus_tag="VIG70_17895"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8252469.1"
                     /translation="MPPGYATAIWPPSGIAVAALLLGGGRLWPAVWAGSFAANITIDW
                     AYAASTIIATGSTLQTLAIAGLVREHIGVLHRFETVAHVVRFVLIAALGCAIAPTFAL
                     LPLGAFYEMSAVELAGNWATWWQGDLCGVLAFAPLILGWLAPRATTWTPGKLLEAGVF
                     GVVLLGASQLVLSAGFERAFLIVPFIVWAAFRFEQREVTTATAAVCAIALWSMPQESF
                     VLLPLFVSTLVFTGLVLSVALKQLERARTELESRVRERTAELEEAKQAAEKANEAKSQ
                     FLANMSHELRTPLNSLLILARLLADNPGGKLDAKQVKFAQTIHASGLDLLSLINDLLD
                     LAKIESGAVTALNVAPARFADLREELERTFQQVAHDKSLAFSIHIDPALPPTLRTDAG
                     RLKQVLKNLLANAFKFTSRGSVALRIAPGAPGYVAFAVSDTGIGIAADKQQLIFEAFR
                     QADGTTSRLYGGTGLGLSISRELTRLLGGELGLASVPGEGSTFTLVLPLSDQRTTLAA
                     "
     gene            complement(52804..53523)
                     /locus_tag="VIG70_17900"
     CDS             complement(52804..53523)
                     /locus_tag="VIG70_17900"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY8252470.1"
                     /translation="MPTLFISDLHLTEERPEATERFIAFLEDKARTAEALYILGDFFE
                     YWIGDDDLGEPFNAVVAGLLADLTQRGVPVSLMHGNRDFLIGERFCAATGAKLLQDPV
                     VETIQGEKTLLVHGDTLCTDDLDYQAWRRKARDPAFQADFLAKPLPERRRAVGQMREK
                     SKQVVQEKTAEIMDVNDEAVRQALRRHGVRRLIHGHTHRPGRHALEVDGQRCERWVLP
                     DWYGRGGYVEVSRRGPRLVRF"
     gene            complement(53537..54112)
                     /locus_tag="VIG70_17905"
     CDS             complement(53537..54112)
                     /locus_tag="VIG70_17905"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8252471.1"
                     /translation="MNLKHLFILSAAAVAPGALAADPQVDVKTSAGTIRLELYPAKAP
                     KTVENFLQYVRDGHYNGTVFHRVIDGFMIQGGGMTASMQQKPTRKPIVNEAQGGIKAG
                     LKNEVGTIAMARTADPNSATAQFFINVADNSFLNWGDPRGDGNGYAAFGKVLSGMDVV
                     TKIAKTPKGPSDVPRQAIVIESMTVVPEPKK"
     gene            complement(54109..55263)
                     /locus_tag="VIG70_17910"
     CDS             complement(54109..55263)
                     /locus_tag="VIG70_17910"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY8252472.1"
                     /translation="MRAVVLSVLLCWAAVAASATPEDRLSTVFQSIEANRLDEALQRV
                     DALIREYPNFRLAHLVRGDLLLARARPLQTFGNVAKTVPGEKIDGLREEALARLRAQR
                     HRPGDDRLPRYVLQLHAEQKHVLMVDSRRSRLYVFGNDKGTPRLVADYYVTLGKNGIE
                     KTREGDQKTPVGVYHVTANLPRKKLTDFYGAGAFPINYPNEWDRRQGRGGYGIWLHGV
                     PSDVYSRPPRASDGCIVLANPDLESVGRHVQVGLTPVIIADEIEWSDAATIAAERAAL
                     AGAFEQWRADWQSRDTERYLAHYSSRFAAPGQDLAAWAEHKRKVNGAKRWIKVGVSRV
                     AMFRYPRERDFVVVTFNQDYRSDGLSNVMRKRQYWVKEGAQWKIIYEGAG"
     gene            complement(55263..56243)
                     /locus_tag="VIG70_17915"
     CDS             complement(55263..56243)
                     /locus_tag="VIG70_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8252473.1"
                     /translation="MLSYAARWGWVLVALAAAMPVCADDVEDAAKLLKAGQHKQALDL
                     VNKVLAAKPRDAQARFLKGLIFTEQGKSKDAIEIFTKLTQDYPELPEPYNNLAVIYAS
                     QGQYDRARAALEQSIRTHPSYATAYENLGDVYAKLASQAYDKALQIDSSNTAAKNKLS
                     LVRELVARPSATAVAVAPKEAAKPQPTPKEAPKEAAKPPVVAVAKPAPEKPAADLSAE
                     ILQAVNGWAEAWSKKDADAYLAYYAKDFKTPGGEARDSWEKTRRARISAPKSISVSLA
                     SPKVTMNGADRATVTFQQNYKSDVLKSNSRKTLTLIRADGGWKILDEKSG"
     gene            56292..57650
                     /gene="cysS"
                     /locus_tag="VIG70_17920"
     CDS             56292..57650
                     /gene="cysS"
                     /locus_tag="VIG70_17920"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY8252474.1"
                     /translation="MLKIFNSLGRAKQEFIPLHPGEVRMYVCGITVYDYCHLGHARFL
                     VAFDMVQRWLRASGYRVTYVRNITDIEDKIIRRAQENGESIDALTSRFIRAMDEDAAA
                     LGVQKPDHEPRATQYVSDMLRIIEALQERKLAYQAASGDVNYAVRRFPGYGKLSGKSL
                     DELRAGERVEVDTSKQDPLDFVLWKRSKPDEPKWPSPWGEDRPGWHIECSAMSSRLLG
                     EHFDIHGGGEDLQFPHHENEIAQSEGASGRKFVNYWMHNAFVRVDEEKMSKSLGNFFT
                     VRDVLKKYDAEVVRFFILRAHYRTALNYSDAHLDDAKAALTRLYTALKDSSTRSGIDW
                     SEAHARRFKEAMDDDFGTPEAVAVLFELASRVNSGEKRLAGQLKGLGGVLGLLQRDPG
                     AFLQAKPAGVSDQWIAEKIVEREAARGRKDYDAADRIRKELLEQGIVLEDKGGKTSWR
                     RA"
     gene            57898..60051
                     /locus_tag="VIG70_17925"
     CDS             57898..60051
                     /locus_tag="VIG70_17925"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY8252475.1"
                     /translation="MSEGKESVAAKPGGQRPPLPPIREDLRLYPGPAHADGSPSWRIL
                     DPVRNSFFEIGWLEFELLARWRGQRDGAALLEQVAAETPLKPSPEELKELIDFLSTNQ
                     LLSPRSAIAQEVLNRRLHESKHAWYLQVLHHYLFFRLPLFRPDAFLARTVGVTDVLFT
                     RGFMVFVLVLLGLDLYLVSREWYSVADAMARMFTPQAFLYYAVAVSFSKVVHEFAHAY
                     AARRYGVRVPTMGVAFLVLWPFLYTDTSETWKLADRRKQLVIASAGMVSELTLAVFST
                     LLWALAPEGGAKNVLFVLASTTWVVTLAINLSPFMRFDGYFVLSDLLGFPNLHERSFA
                     CARRWMRATFFGLDEPLTEPGLRPRQRAGLIVFAWLTWLYRLTVFLGVALLVYHIAFK
                     LLGIFLMLVELIWFIARPVWKEIGYVWKARSTVKMAWRPAIVLLALITALVWLIPVSY
                     EVTAPAILRAQEEHAVYAPFPARVTAVRVSDRQRVGADVELVSLEAVDLDVREKKADI
                     SIVAARAELARMPASVRLQENYGVLQERLAQALAEKQAVRDEYGRQQLRAAQAGIVRD
                     LAPDLLAGRWINPRQLLMRVVSEDVPLIEAYVSERQVAAIVPGQSVRFFPQLPDRPVL
                     TGEVVAVDKSPQKQLSRPLLASLHGGAVVVKQDQHGSLVAQDALFRVIVKPVGAPPKV
                     DAVIHGSVRIETGLRFLVENFVYRILSVLIRESGI"
     gene            60065..>64843
                     /locus_tag="VIG70_17930"
     CDS             60065..>64843
                     /locus_tag="VIG70_17930"
                     /inference="COORDINATES: protein motif:HMM:NF025611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HEY8252476.1"
                     /translation="MSKKSDRESKKAVRRKTSAAPTKRRPPRRTRMLALEPRMLFDGA
                     LGIDLGAKAAAALQGDAAAPAESTTPAAPQAQAGAPAAPAAPEKSAVEKTAERLGIAP
                     EAPVRNEIVFVDSTIQGHEALIAGLSPTAKVVMLDPTRDALDQIADYMDGERNVDAIH
                     VLSHGSAGNLILAGQTYSADRLTQEYATDLARIGHALSADGDILLYGCDVGKGSAGER
                     FIDTLSAITGADIAASVDKTGAADLGGDWMLEVKTGQVETVSISLTHEWDFLLTAPPV
                     NVGGATLDFDGANRTLVSGVAGAVGAVYKYADVATINGVQIDAYVTISGITNATLVNI
                     DNDAPASYVPPAGTNAANVFAPEISVTAANGKIDFAINFKDPNGNNLTLLNFYNNSLD
                     IDGAGGFQEFVEYGGFKSVTTGNPTDLVISSSPGNDRLRFTGSSVYNGLIVNDVGRVQ
                     ANFDAISTLQISMGATGNTGGLRQYGSLFSAVSFGSPVTITAPTSDLLTTADTTPILT
                     GSVGAAPLGGGETFSVTVNGTTYTTANGLGISGSTWSLELPTALASGQYDVRAVRSSA
                     GLDIADQTQLELTINDAPTLADTALTLTVAEDAPAPAGAVGSLLSSFTGGISDVNAGA
                     AKGIAITGTNETNGTWYYTTNGGTTWTAVNAIDGTVSGTNALLLADNANTRLYFRPNA
                     DYNGSAPAALTLRAWDTTSGTAGTKVDPGSGGGSLAFSTASDVIDVTVTPAADAPVNT
                     VPGAQGTGLNIPLIFSTANGNVLSAADVDGNLATVRVTVTNGVANVTLFGAATISAGA
                     NGTNTLTISGTQADINATLNGLRYTPTAGFTGAATLTLLSTDSAAQTDSDVVNINVSA
                     VAALPRIDLNDGSASDTIADNFASGGYAGSSGSVNLWTGSWVESDPEGAAQSAAAGDV
                     RVTAGALVLGDPGADNGVVAANRASIARALDLSNHINASLSFNYNRANLEAGDTMLVE
                     VSTNGGATYTTVGTFPGSTAVGVQNVSLAGYESANTLIRFRLSTDFATNETVTIDNVT
                     ITAEPTGYTNTFTEAVTYVAGAGGAAIAAADTLITDPSGGNLAGAMIRIATNYQSGAD
                     LLEFTNQGGITGVFNAATGTLTLTGNATAAVYTTAIESIRFNNSSGAPSTSPRTITVV
                     VTDTTGAASNTAKSTINVVATNDAPVGVNDASTAIEAGGVANATPGLNPTGNVLTNDT
                     DVDAGDTKTVSAITGGTVGVAKAGSYGSIVLNANGSYTYTVDNSNAAVEALRSAASEL
                     YDTFTYTVRDTAGLTSTATLTITIQGANDTATIGTPSVAVVTEDVGVSGGNLSATGSI
                     AISDVDDHGAIFSTIVVPVGSPLGALVLAADGTYTYTVSNAAVQSLGAGQTAIDTFMV
                     TSADGTTQNVSFTINGANDAPVNAVPGAQAVNEDTALSIGGVSVNDIEGNLSTTRLTV
                     TNGAVTVSLAGGATISAGANGSSTLTLSGTQAQINAALASISYQGNLNFNGGDTLTVL
                     STDSNGATDSDAVAITVNPVNDGPVNTVPAAQSVNEDTALSIAGVSVNDVDGNLSTTR
                     LTVTNGAVTVSLAGGATISAGANGSSTLTLSGTQAQINAAL"
BASE COUNT        11020 a        21749 c        22181 g         9893 t
ORIGIN      
        1 gatgccgccg ccagtacgga tcttctggtt ctggaaatcg gccagcgact tgatctgctt
       61 cttggtgaac atctggccgg gaccgtgggt ccagaccgcc agcagcttga cgcccttgta
      121 ctcgccgacc ttctggaaat gcttccagtg gatgcgcgag aaggccaccg agttgatctc
      181 ggcggtctcg ccggcgcccg gcagctcggc catcagcggc aggatgtggc gcgccggcgt
      241 gtagctcgcc gtcacatagg agagatccac caggccgtcg cgcaccgcgt cgaaggtgcc
      301 cggcgcggcc gctggatgct tcggcagcga ctggaacttg acgcgcccgc cggtcgcctt
      361 ctcgacgtcc gcggcccagt tggcctgcca gatggtgagg tggtgcgtgg gcggaaccca
      421 gctcgagaac gtgagcgggg cttgcgcgtg cgcgggtagg gcgagcgcga tgagcgccaa
      481 tgccagtgac ctgatcatgc aacctcctcc ttgaggtgtc gtaatcataa atgtttgtcc
      541 tgcggcgtgt gcaaattcac gacacccggc gacgcgcgcg ttgtatttcg attttggttt
      601 ggatacgatt ccgtgctcgc ggttggggat gaaagtcagg ggagagcgca tgccacacgt
      661 ggttaccaag tccgatctgg tccaggttgc ggcaaaagcg gggaacgtca gcaaggtggc
      721 ggcaaacgaa gcggtgaatg cggtgttcga ggcgatcgtt tccaacgtcg cgaagggcaa
      781 gcgcgtgacc gtggtcggtt tcggcacctt tcttccgcgc accagaaagg cgcgggccgg
      841 gcgcagcccg gtgaacggga aggagatcaa gatctctgcg aaaacaattc cggcgttcgc
      901 cgcgggccag ggcttcaagg aagcagtcgg caagtagagc cttacatgat ccgcaagggg
      961 ctgagcattg catgccagcc ccttgttttt tttggcattg ccaagcagaa tcctcggcaa
     1021 gcatgaatct tgcgcatgga taagccgctc attctttcgc tcgacgcgca cggcgtcccg
     1081 caccgctgga tcagctggca gcaggcgtgc ttctactacg cgaaggacct ggtcgcgtgg
     1141 acgctcggcg aggagaagtt cacctactac ggcggcatct gccgccgtac cggcgagcgc
     1201 tcgagcatca ccgcgcattc catcgtcgcg atcaagggca aggcgctggc ggcgaaaggc
     1261 ttcaaccagg tgccgccgct caacaaccgg gaactgttcc gccgcgaccg ccacatgtgc
     1321 gcctattgcg gcggcgagtt cagctatttc cgcctgacgc gcgaccacat cacgccgctg
     1381 tcgcgcggcg gccgcgatac gtggatgaac gtggttacca cctgccggca ctgcaacggc
     1441 cacaagcgca accgcctgcc gcaggaatgc ggcctcgagc tgctctatgc gccgtacgtg
     1501 ccgaacaagg cggagtacct gatcctcacc aaccgccgca tcctcgccga ccagatggag
     1561 ttcctgaagc agcatgtcgc gagccactcg cggcttgtcc tagcccctgc gtagcttgtc
     1621 tgcctgagtt ttggcgcaat agcggttttc accttctgaa gcgggatccg gccggtcgat
     1681 tgaccgtcac cgacgatttc ctgcgcgcct actacctgcg gccggagatc cacccggtcg
     1741 aggaatccgg cgacgccgag ctcaggctgc actcgtcgct gatgaacgcg ccgcgaagaa
     1801 gcgtcggcga aaaggagctc gaggcgatcg aggatgccga tgcgcgcgac aactaccgcg
     1861 tcatcctgaa gttcaggcag aagctgctcg atgcgggcac ggtcgagggc tgctacaggg
     1921 gcctcttcaa gaccggcgtc gacgtgccgc cgatgttcgt cgaccagctc gtgcatgtca
     1981 tcctgcgcaa cgttctcgac ggctgcgacg acccgctgaa gctgcgcgcg gcggagcttt
     2041 tcttccggga gcagaaggcc accatccagg aaggccacgc cctgctcgcc gacctggaga
     2101 ccgtcgagat gcacgccagc ggcaaccgct acggagcgat cggcaagctg atcgtcgagg
     2161 cgcagggcga cctcggaaaa gtcgacctcg atgtcctcga ccgcgccaat gccgcgctct
     2221 actgggagcg cgactcgcgc cacgacacgg tgatcagcct cacctacggc cggccggcgc
     2281 tggatgcgct ttgccgggtg atggaagcct gggtgtctca ttttctgaaa accaaagtcc
     2341 aggtgaaacc cattcgaaag atcgacgagg cgccctgggc gtggcacgtc gggctcgacg
     2401 ccgagtcgac tgcgatcctg aacgagctgt ggtcgggcgc gcagatcgag cagggccgga
     2461 tgcgcaacat cctcgccatc ttcggcctgc agttcgacga cccggcggcc atgcgaaccg
     2521 acctccgcgg caggacgatc tacctcgcgc tgtcgtgcga ggacggggtg gtgcggatga
     2581 agccgcagaa cctgctgacg aatcttccgc tgcatgaagc ctgattactg ggatcgcgcg
     2641 aagcgcgccc tttccaggaa cgatgcggtc atgaaggaaa tcatgcgcag ccggcggggc
     2701 gtccacctgg tgcgtcgcgg cgagcccttc ctcaccctgg cgcgcgcgat ctgcgggcag
     2761 cagatctcgg tcaaggccgc gcagagcgta tgggacaggg tctgcatttg cctggaccaa
     2821 aaaatcactc ctgaggcggt tattgccaaa gacagaaagg tcctgcgcgc ctgcgggctt
     2881 tccgaccgca agaccgagta catcgccgac ctcgcccagc acttcgccga cggcagcatc
     2941 cacgtccacc gctggccgca gatgccggac gaggagatca tcgccgagct ggtcgaggtg
     3001 cgcggcatcg gccgctggac cgcggagatg ttcctgatct tcaacctgct gcgtcccgac
     3061 gtgctcccgc tcgacgacct gggcttgcag aaggcgatcc ggcttttgta ttttcgaaaa
     3121 agacccgttt ccctgcggac tatgcgcaag cttggcgaaa gctggcggcc gtggcgctcg
     3181 gtggcgacct ggtacctctg gcgctcgctc gatccagtcc cggtagaata ctgaccccgt
     3241 tgaagaccac cttcctcgaa ttcgagcagc cgatcgcgga gctcgagacc cgcatcgagg
     3301 agctgcgctt cgtccaggac gactcggcgg tcgacatctc ggaagagatc cagcgcctca
     3361 ccaagcgcag ccagtcgctc accaaggaca tctacggcaa gttgagcccc tggcaggtgg
     3421 cccaggtggc gcgccacccg cagcgcccgt tcacgctcga ctacgtccag gcgctgttca
     3481 cgcacttcga ggagctgcac ggcgaccgca cgttctccga cgatgcctcg atcgtcggcg
     3541 ggctcgcgcg cttcaacggc acggcgtgca tggtgatcgg gcaccagaag ggccgcgaca
     3601 ccaaggaaaa gatcctgcgc aacttcggca tgcccaagcc cgagggctac cgcaaggcgc
     3661 tcaggctcat gaagctggcc gagaaattcg cgctgccggt gttcacgttc gtcgatacgc
     3721 cgggtgcttt tcccggcatc gatgccgagg agcgcggcca gtcggaggcc atcggcagga
     3781 atctgttcga gatggcgcgc ctgcgctcgc cgatcatcgt caccatcatc ggcgagggtg
     3841 gctcgggcgg cgcgctcgcc atcgccgtcg gcgacatcat gctgatgatg cagtacgcca
     3901 cctactcggt gatctcgccc gagggctgcg cctcgatcct gtggcgcagc gccgacatgg
     3961 cgcccgatgc ggccgacgcg ctgggcatca ccgctagtcg cctgaagacc ctcggcctga
     4021 tcgacaagat cgtgcccgag cccctcggcg gcgcgcaccg cgacccgcag gtcgcggcgc
     4081 aggcgctgaa gaaagcgctc gccgaagcgc tcaagcagct ccaggagaaa aagcccaagg
     4141 agctggtcga gacccggctc gagcggctga tggcccatgg caagttcaag gaagcggccg
     4201 agcggtaggg gcattgcggt cgccctgtcg ggcggcgtcg actctgtagt tctgcttcac
     4261 aggctaagga acgagccggg cctgcgcgcg attcacgtcc accacgggct aagcccgaac
     4321 gccgatgcat gggccgcctt ctgccggcgc ctgtgcaagc gcctcggcgt gcctctcgtc
     4381 gtcaagaagg tgaaagtggt gaagcaaggc gagggactcg aagccgcggc gcgcgcggcc
     4441 cggtatgagg tgttcaggaa aattcccgcc gaggcggtgg ttctggccca tcacctcgat
     4501 gatcaggccg agacggtcct catgaacctg ctgcgcgggg cgggacggcg cggggcgagc
     4561 ggcatgccgc gcgagtcgcg gttccatggg aagcggctgc tgcggccgct gctggatgtg
     4621 ccgcgcgagg cgatcgtcgc ctacgcgcgc gagcacggcc tgacctggat cgaggacgag
     4681 agcaatgcgg acgaatcgct gacgcgcaat ttcgtccggc ggcggctggc acccctgatg
     4741 cagcagaaat atccgaagtg gcgtgaaaac ctggcgcggg cggcgcggca cttttcgcgc
     4801 caggaagccg gcgcgcaaga cctgctgcgg aaattcctgc aagcgaaggg cctgcgcgcg
     4861 ccgagcgagg cgaagctggt cgagatgctg aagcagctca cgtcgcaggg cagcaagacc
     4921 ctgatcgagc acgacggcgc gcgcctgcgc gtatacaggg gaaagatcat ggaagatcgg
     4981 ggcgcgggct cgttcagccc gattccatgg aagggcgagc cgaagctccc gattcccgag
     5041 ctcggcggcg agctgcgctt ccgcgcggtg cgcggtaagg gcttttccaa gaacctgttc
     5101 aagaagaccc tcagcatccg gttgcgcgcc ggcggcgagc gcctgcagcc ggacccgcgg
     5161 cggccgcggc gcacgctgaa aaatctgttc caggaagcgg gcgtgccgcc gtgggagcgc
     5221 gaccggctcc cgcttctcac cagcggcgac gacgtggtgt gggtccccgg gctcggcgtg
     5281 gatgtgaaat accgcgcggc gaaaaatgcg tccggcatcc tgccggaatg gcgtaaaatg
     5341 ccgggttaat gcgttgattt cccaaggaga atgcagtcaa tggccgtgga gcagaccctc
     5401 tcgatcatca agcccgacgc ggtcaagaaa aacgtcatcg ggaagatcct ggcgcgcttc
     5461 gaggccgctg gcctgcgcat cgtcgccgcg cgcatggcgc atctgtcgcg caaggaagcc
     5521 gaaggcttct acgcagttca ccgcgaacgt ccgttcttca aggatctggt cgagttcatg
     5581 atttcggggc cggtgatgat ccaggtgctc gaaggcgaga gcgcgatcgc gcgcaaccgc
     5641 gagctgatgg gcgccacgga cccgaaaaag gccgccaagg ggacgatccg cgccgacttc
     5701 gccgattcga tcgacgccaa cgcggtgcac ggctccgacg ggcctgagac cgcgcgcaag
     5761 gaaatcgcct acttctttcc gaaggtcgat gagagaaaac ctgctcgggc ttgacgcggc
     5821 cggcctcgag cgcttcttcg aagcgcaggg cgagaagccg ttccgcgcgc gccagctcct
     5881 gcgctggatt caccagcgcg gcgagggcga cttcgcaggc atgagcgacc tcgcccgagc
     5941 gctgcgcgag cggctgccgt ctatcgcggg cgtggaggcg ccggtgatcg tcggcgacac
     6001 cgcggcggca gacggcactc gcaagtggct gctcaaggtc gatcgggcga acgcggtcga
     6061 ggcggtgttc atccccgaga cgcggcgcgg cactctgtgc gtctcgagcc aggccggttg
     6121 cgtcctcgat tgcgccttct gctcgaccgg caagcagggc ttcaaccgta acctctccac
     6181 cgccgagatc gtcggccagc tctggctcgc gaaccgcctg ttgaagggtg ataatcacgg
     6241 cgaaagacct gtgaccaacg tggtcatgat gggaatgggg gagccgctcc tgaacctgga
     6301 caacgtgatc ccggcgctgc ggctgatgct cgacgacaac gcctacggac tttcgcgccg
     6361 ccgcgtgacg gtctcgaccg ccggcgtgat cccggggatc gagcgcctgc gcgacgaatg
     6421 cccggtggcg ctcgcggtct cgctgcacgc gcccaacgac gagctgcgcg accgcctggt
     6481 gccggtgaac cgcaagtatc cgctcgccga gctggtgaag gcgtgcaagc gctatctcga
     6541 caaggcgccg cgcgatttca tcactttcga gtacgtgatg ctcgagggcg tgaacgacgg
     6601 cgacgcgcac gcccgcgagc tggtggcgct cgcggcgaag gtgcgcagca agttcaatct
     6661 catcccgttc aacccgttcg cgaattccgg cttcaagcgc tcctcgcccg agcgcatccg
     6721 gagcttcgcc gagatcctgc agcgcgccgg gctgacggtc acgatacgca agacgcgcgg
     6781 cgacgatatc gctgccgcct gcggccagct ggccggcgat gtcacggacc gcacgcgcag
     6841 gagccttgcc ctcaaggagc tgcgcgcttg aaacggcttc ttcttgtcat gcttctggcc
     6901 ggctgcgccg cgaaggacac cggtccttcg caggagagcg tgtcgatcat cggcgaggcg
     6961 gcggacccgc gcaaccgggc gaagctgcac accgacctcg cttcgctcta cttcggcgcc
     7021 ggcaacttcg gggtcgcgct ggaggagctg cgcctggccc agtcggccga ttcgaactac
     7081 gcgccgacgc acgggatgtt cggcctgctg tacatggagc tgaaggagaa cgctcgcgcc
     7141 gaggagagct tcgagcgcgc gctgcgcctt gcgccgaacg actccgacat caaccacaac
     7201 tacggctggt tcctgtgcca gaccgggcgc gagccggcgt cgatcaagta cttcctggtc
     7261 gcggtccgca acccgctcta cacgacgccg tggcgctcgt attcggcggc cggggtctgc
     7321 acgctgaagc tcgacgagtc caagtccaaa tcgaaggaga aggacgccga ggcgttcttc
     7381 gagcgcgcgc tgcggctcga gccggacgag cccgcgtcgc tgctgcagct cggtcagatc
     7441 cgttaccggc agggcaatat cgccgatgcg cgcaagctgg tcgcgcgcta caacaaggtc
     7501 gccaacccga gcgccgagtc gctgtggctc gcggtgcgca tcgagcgccg gctgggcgag
     7561 cgcctccagg agcaggctta cgccaaccag ctgcggcggc gctacccggc ttcggcggaa
     7621 aaccagtctt tgcagcgcgg gcgatatgac tgaagcccaa gctgcggaga tgcgcactgg
     7681 cgtagggcag gagctcgcgg cggcgaggga ggcgcgcggg cttgcgctct ccgacgtcgc
     7741 gcagcagctc aagttcgcgc cgcgccagct cgaggcgctc gagcaggagc atctcgacga
     7801 gctgccgggc gcgaccttcg cgcgcggcat ggtgcgcagc tacgcgcgcc tgctcaagct
     7861 cgaccccgag cccctggtgc agcgcatcgc cgggcgcttc gaggttccgg actccaaccg
     7921 gctcgcggcg cgctaccacc agccggtgcc gttctccgac ggcgcgcggc gctcgacttt
     7981 cgtctatctc gcgatgtcgc tcggcgtgct ggtgctggtc ggcgcagtcg cctacgagtg
     8041 gcaccaggag cgcaccgcgg ctgcgcgcag taccaccctt ggggcaacca gcgtcgccaa
     8101 ggcaacgccc gcgccgcgcg aggaaaagcc gcacgcggcc ccgatcgccc ccgtgccggt
     8161 gcagcaggcc acggcaccgg cgccggcggc gaccgaaaag actccgaaga cggtggaaaa
     8221 gccgccgaag accatcgaaa aagcacccct agccgccgtc ccggcgactg catccgggac
     8281 gggtgccaat cgcctggtgg tgcgcaccga gggtgaagcc tggatcgaga tcagggattc
     8341 cgccgaccgc atgctcatat cctcgctgaa tcccccgggt agcgagcgcg tagtgcgcgg
     8401 caagcctccg tatagcctgg tcatcggcaa tgcgtcccag gtgagcatca cctacgacaa
     8461 caagccggtg gacctcacgc cgcacacccg gcaggacgtg gcgcgcctga ccgttccctg
     8521 agagccatgc aacgacgaac ttcgcggcag gtcgccgtcg gcggcgtgcg catcggcggc
     8581 ggagcgccga tcgtggtgca gtcgatgacc aacaccgaca ccgaagacgc cggtgcgacc
     8641 gcgcgccagg tcgaggcgct cgcgcgcgcc ggctcggagc tggtgcgcat cacggtcaac
     8701 acacctaaag ctgccatcca agtgccgcgc attcgggagt tgctggattc ggtgggctgc
     8761 gcggtgccgc tgatcggcga cttccactac aacggccaca agctgctcac gcaatacccc
     8821 gactgcgcgc gggcgctggc gaagtaccgc atcaacccgg gcaacgtcgg caagggcgcg
     8881 aagcgcgacg agcaattcgc caccctcatc gaatgcgcgc tgcgctacga caagccggtg
     8941 cgcatcggcg tcaactgggg cagcctcgac caggtgctcc tggcgcagtt gatggatgaa
     9001 aatgcaaaaa aatcgattcc ttcggatgcg agcgaagtga tgaaggaagc ggtcatccgt
     9061 tctgccttgg aatcggcagg acaggccgaa gcctggggcc tgcccgccga gcgcatcgtg
     9121 atctcgtgca agatgagcca ggtccaggac ctgatcgcgg tctaccgcgc gctcgccgca
     9181 cgctgcgaat atgcgctgca cctcggcctg accgaggccg gcatgggctc gaagggcatc
     9241 gtcgcctcga ccgccgcgct ctcggtgctg ctgcaggaag gcatcggcga caccatccgc
     9301 atttccctca cgccggagcc cggcggcgat cgcgcgcgcg aggtgcaggt cgcccaggag
     9361 atcctgcaga cgatggggct gcgcgccttt accccgatgg tcagcgcctg ccccggctgc
     9421 gggcggacaa ccagcaccgt ttttcaggaa ctcgcgaaaa acatagaagg cttcttgagg
     9481 gagcagatgc cagtctggaa aagccagtat cccggcgtcg aagagatgcg cgtcgcggtg
     9541 atgggctgcg tggtcaacgg cccgggggag tccaagcacg ccgatatcgg catcagcctt
     9601 ccgggcaccg gcgaagtgcc ggtcgcgccg gtctacgtgg atgggaagaa gaccgtgacc
     9661 ctcaagggcg agcgcatcgc ggaggaattc caggcgatcg tcgaggacta cgtcaggaag
     9721 aaatacggga agcgcatccc tatcaaggcg atctgacgtg cagaaggtaa aacctgcctt
     9781 gctgcgcggc ttcgaccagg agtacctgcc gcacgatcag ctccacttca accggctgat
     9841 cgacatcatc cggcgcaact tcgagctcta cggcttcctg ccgatcgaga ccccgtcggc
     9901 cgagcgccgc gaggtgctca cctccaaggg cggcgtcgag aaggagatct acgcgctgac
     9961 gcggctggcg gaggccgacg acgacgaggc cgcgaccaag ggcgcgctgc gcttcgacct
    10021 gacggtgccg ctcgcgcgct acgtagcgat gcgcgagcgc gagctcgcgt ttccgttccg
    10081 ccgctaccag atccagcgcg tctggcgcgg cgaaacgccg caggcgcgca aggggcgctt
    10141 tcgcgagttc tatcagtgcg acatcgacat catcaaccgc gacaagctct cgtacctcgc
    10201 cgaggccgag atcccgagcg tgatctacag cgtgttccgc gagatggcga tcggcgagtt
    10261 ccgcatccgc atcaacaacc gcaaggtgct caagggtctg ctgcaaagct tcgacatacc
    10321 ggagcgcagc tccggcgcgg ttctgcgaac cttggacaag atcgagaagg aagaggcggc
    10381 gaagatccgc gccgacctcg agcgcgaagg cctttcgcgc gatgccgccg gcgagctgct
    10441 cgacatgatc tcgacccggc gggacacgaa ggccaccctg gaagcgctcg cccggatcaa
    10501 gaacgagacc gcggcgcagg gactcgacga gctgcgcaag atcgtggagg cgatcggcca
    10561 gttcggcgtg ccggactcgg cgttcgccgt cgacctcggc gtggtgcgcg ggctcgacta
    10621 ctacaccggc acgatctacg agaccacgct cgccggcgcc cccgagctcg gcagcatctg
    10681 ctcgggcggg cgctacgacg acctggcgag ctatttcacc gatacgaagc ttccgggcgt
    10741 ggggatctcg attggcctca cccggctgtt ctcgaagctg aaggaagccg gcctgctcca
    10801 gccgctgccc aggagcccgg cggaggtcct ggtgacgacc atggacccgc gctacctcga
    10861 caagtatttc gctctcggcc gcgtgctgag agaggcgggc atcaataccg aggtctatct
    10921 cgagaccgcc aagctcggca accagctcac ctacgctgac cgcaagggct tccgggttgc
    10981 gataatcgcg ggcgagaacg agttcgccaa ggacgcggtc cagatcaagg atctgcgcgc
    11041 gaaaacagcc cgcgatgtgg cgcttgccga aatcgttccg tcggttcaag ccacgttggg
    11101 tggcagtgat tgaggttcaa ctgggacttg gcgaaggccg aggagaactt ggccaagcac
    11161 ggagaggttc gtaatcttcg gactctccag cagtggccgt ctgatggtcg tcgctcacgc
    11221 tgagcacaac gatacaattc gcatcatcag tgcccgactc gcgacggcgg gcgaaaggac
    11281 catctatgaa gaaggctagc ggcaagtccg acgaagcgct gcgaccggag tacaagcgct
    11341 ccgatttcgg tacattggtg cgcgggaaat acgcgcgcag agtcgccgaa gcgaccaacg
    11401 tcgtcgtgct ggaaccgcag gtcgcgcagg cctttccgaa cgactacgcg gtgaacaagg
    11461 cattgaaagg ccttctgcgc ggacgccggc aaagcgcacg gccgacggca cgtgcacgga
    11521 cgcgccgcga acggcgcacc gactaacacg actcgaagag ccaatgcccc tcgatctcga
    11581 agaacaggaa caggtcgccg agctgaaagc ctggtggcgg cagcacggca atctcatcgt
    11641 ctcggcggtg ctcgccgccg cgctcgcctt cgccgggtgg cagggctggc gctggtacag
    11701 cgcgagccag gcggcgcacg ccgcggcgca gttcgaggcg ctcgccaagg cggcgcaggc
    11761 gggcgacgcg aaggcgctgc gcgatgccgc gggcacgctg atcgagagct atccgcgcac
    11821 gctctacgct tcgatgggcg cgctggtcgc ggcgcgcttc cacttcgacc gcaacgatct
    11881 caaggcggcc aaaacccagc tccaatgggt ggtcgagagc gcgccgtcgg aggatttccg
    11941 cgacctcgcg cgcctgcggc tcgccgccat cctgctcgac gagaaggcgc acgacgaggc
    12001 gctgaaggcc ctggacgcaa gacattcggc ggcctacgac tcgcagttcg ccgcgttgcg
    12061 cggtgacgtg ctggtggcca agagccagcc ggccgaggcg cgcgccgcct accagctcgc
    12121 gctggacaag gccggcaagg agcagaacgc attccgcgag agcgtgcgca tgcggctcga
    12181 ggcgctcggg ggctgaacgt gaagtggaag gcagggatgg tcatgaccgg gatcgtcttc
    12241 gccggctgcg cgagcgaccc gggtccgaag ccggccgagc cgccgcgcat cgagaaggcc
    12301 caacgcgtgc gcaccctgtg gtcggcgagc gtcggcggcg ggcagggctt cactttccag
    12361 cccgcgctcg ccgccggatc ggtatacgcc gcggcacgcg acggcacggt gatgcgtttc
    12421 gacgagaaga cgggcgacac gcgctggcga gccgagctcg agctcaagct ttccggcggc
    12481 gtcggcgccg acgagctcac cgtggccgtg gcgagcgaag agggcgaggt cgtggcgctc
    12541 gacgcgcaga ccggcaaagt gcgctggcgc gcgcgcgtct cgagcgaggt gctggccgcg
    12601 ccggccatcg gcgccgggct cgtgctggtg cgcagcatcg acaaccgcgt gttcgccttc
    12661 ggcgcggatg acggcaagcg ccgctgggtc taccagcggg cgccgtcggc gctcctggtg
    12721 cgcgcgccgg cgggcatcac gatcgacggc gggctcgctt ttgccggctt tccgggcggc
    12781 aagctcgccg cgctggcgtt ggagagcggc gcggtgcgct gggaagccac cgtagcggta
    12841 cccaagggca cgaccgagct cgagcgcgtc accgacgtca tcggtagccc cgcggcgcag
    12901 gggcgcgagg tatgcgccgc ggcgtaccgc agcaggctcg cctgctacga cgtcgccacc
    12961 ggcaggcagg tctgggcgcg cgagctcacg tcgctttccg gggtcagcct ggacgcgcgc
    13021 tatgccttcg tggccgacga ccgcggcacg gtacatgcgc tcgaccgcag caacgggcag
    13081 tccgtgtgga agcaggacaa gctcgcgtac cgccagcttt cgacgccgct cgcaggcggc
    13141 gagtcgatcg cggtcggcga cttcgagggg tacattcact tcctcgcccg cgataccggc
    13201 gtattcgtcg cgcgcggcga gaccagcggc cgcgcggtgc gcgcgacgcc gctgcgcctc
    13261 ggcgccgggc tgctggtcca gaccgaagac ggcggcttgt ttgccctctc gctgtgaagc
    13321 cggtcatcgc attggtgggc cggcccaacg tcggcaagtc caccctcttt aaccgcctca
    13381 cgcgcagccg cgacgcgatc gtgcacgacg tgcccggcgt gacgcgcgac cgccactacg
    13441 gcgaaggacg gctcggctcg cgcgcgttca tcgccatcga caccggcggc ttcgagccac
    13501 gctcgcccca gggcatcttc gtccagatgg cgcgccaggc cgagcaggcg atggtggagg
    13561 ccgacgcgat cgttttcctg gtcgacgggc gcgccggcct tacgccttct gaccgggaaa
    13621 ttgcacaaaa attacgcaaa ctgaaaaccc cggcgtttgt cgccgtcaac aagaccgagg
    13681 ggatgcaatc cgacatcgcg gtcgccgagt tccacgagct cggcctgggc gcgcccgccg
    13741 cgatctcggg cgcgcacggc gagggcgtgc gcgaattgat ggatctcgtc ctggagaaat
    13801 ttccggagga caccgaaccg gacgaagaag aaaaaggcca tccccgagtc gcggtggtcg
    13861 gccgccccaa cgtcggcaag tcgacgctcg tcaacgcgct ggtcggcgag gagcgcgtca
    13921 tcgccttcga ccagcccggc accacgcgcg acccgatcga ggtgccgttc gagcgcggcg
    13981 ggcggcgcta cacgctgatc gacaccgcgg ggctgcgccg gcgcggcaag accggcgagc
    14041 cggtcgaatt cttctcgatc gtcaaggcgc tgcaggcaat cgaggcggcg aacgtcgcga
    14101 tcctgctcct cgacgcctcc accggcatca ccgagcagga cgcgcacgtc gcgggctacg
    14161 tgctcgagcg agggcgcgcg gtggtgctgg cggtgaacaa gtgggacgca gccgacaagg
    14221 agctgcgcga gcggatgaag caggagctcg cctggaagct cgagttcctc ggtttcgcgg
    14281 aaacgcattt cgtctccgcg acgaaaggca agggcctcgg cccgttgatg cgctcggtcg
    14341 acgccgcgca cggcgcggcg atggccaggc tcgccacgcc caagctcacg cgcgccatga
    14401 tcgccgcggt cgagcgccag tcgccgccga agaccgggcg ggtgcgtccc aagctgcgct
    14461 acgcgcacca gggcggcatc aacccgccgc gcatcatcat ccacggcaac gcgctcgacc
    14521 gcgtgccgga gacctacaag cgctacctgg aaaactactt caggaatgcc ttcaagctgg
    14581 ccgggactcc gctgcggatc gagttccgca ccgggcgcaa cccctacgtc aagtcccgcg
    14641 ccgcgcgagg atgaggctgc gcacgagcgc cggcaggtcg aagccgcgcg agaggagctt
    14701 ccagaagatc atgcgcgcga tgcgcgccgt gtcgacggtc gagcgatagt ggcttgggcg
    14761 cgcgtccggc ggatagaggg cggcgatcgg cacggcggcg ctacgcacgc cggcgcggcc
    14821 ggcttcgatc aggatctcgc tctcgaaggc gaagcgggcg ttggggccgt ggaagagctg
    14881 gatttcgcgc aggacgctgg cgggatacag gcgaaacccg gactggctgt cggcgacggg
    14941 gaagcccgcc gcccaggcca cccagaagtt ggcgaagcgg ttgccgaggt agcgcaaagg
    15001 cggcactttc tcccggtccc agagccgcgc gccgatgatg atgcggtccg ggtgcgcgcg
    15061 cgccgcgtcg atgaggcgcg ggatgtcctc ggggtcgtgc tgcgcgtcgc cgtcgagcgt
    15121 gaccacggcg tccgcgccgt cggccagcgc gagcgcgaag ccgcgccaca ggctggcgcc
    15181 cttgccgtag ttgaccgggt tcgagatgac cgtgacgcgc aggccgtcga gcgcggcggc
    15241 ggtggcgtcg gtcgagccgt cgttcaccac gatcacgtcg ggcacgaggc gcagcgtccg
    15301 ggtggcgacc tcgcggatgg tcggtgcctc gttgtaggcg ggaatgacgg cgacgagctt
    15361 catgtcatgc cgccatcgat cgagatgacc tggccggaga tgtagccggc ttcgtcggag
    15421 gcaaggaagc ccaccagcgc cgcgacctcg tcgggctggc cggcgcgctt catcggcacc
    15481 agcgcgtcga tggcgctctt ggtgaacgcc tgtgcggtca tgggcgagtc gatgatcccc
    15541 ggcgccaccg cgttcaccgt gacgccgcgc gaggcgagct cgagcgcgag cgagcgcgtg
    15601 gcgccgtgca ggccggactt cgccgcggcg tagttcgcct gtccgcggtt gccgcgcagc
    15661 gcggcgaccg aggacaggct gacgatgcgt ccccagcgcg tgccgatcat cggcagcagg
    15721 agcggccgcg tgacgttgaa gaacccgtgg agcgagacgt cgaccacgct cctccactgc
    15781 tctcccgaca tcccggccat cggcgcatcg gcatgcactc ccgcgttgtt caccacgatc
    15841 tgcaccggcc cgtcggcgag caggtgctcg agcgcagccg ctgttgcttt ctcatctgtg
    15901 acatcaaaag aaatcgcgtc ggcggcaccg cccgcagcgc ggatattctg cgccacgcgc
    15961 tcggcttcgg ccaaattgcg gttcgcgtgc acggcgacgt gccgccccgc ggcggcgagc
    16021 cggcggcaga tcgcggcgcc gatggtcccg ctgccgccgg tcacgagggc cctgatcatc
    16081 cggagagcac gacggcggcc ttgccgcgca ggatctcgac gccgccgcag gacagcgcaa
    16141 accggtagag cgcccgctcg ccgtcgccgg caagccgctc ggcatcgatt accagctcgt
    16201 gctcgagatc gtccaggcga gcggcgctgc agacgacgtc gctgacgctc gcgagatagc
    16261 ccgcgtgcgg cgtttcccgg accgcggcgg acaagcgccc atgcaccgcc atggcttgcg
    16321 ccgcgtactc gatgccgcac agcgccggca gctcggcccc ggcgcgcagc ggattgtcgc
    16381 cgtcgcggtg gctgcggctg gtgcatcgaa tcgcagcttc gctccattcc aatacctcgt
    16441 gcagcagaca catccgcccc gcatggggaa tcatgcccgc gatctctgcc ttgcttacgg
    16501 gcatggcgtg actcgcgcgc gcagctgcgt ccccgcgaca tagttcagga cgacgtcccc
    16561 ggcgacgccg cgcgcgagca cggcgagcaa ggcgaggctg cgcgccgccg ggttgccttg
    16621 cctcgcggcc tcgagggccg ggtcggcgag ccgggtctcg gcgccatccg caggccgaag
    16681 ctcgaggtcg atggcggcga cgctgtggcg cgtttcttcg ggaaccagga tgagagcgac
    16741 gccgaacacg ctcgcgatcg ggcgcgcggc gtgcaaaggc tcgggatacg gctggtcgtg
    16801 gctgacgagg gccacgggcc tgcggtccac ggcgacctgg gccgcgcact cgaggaggcc
    16861 cgcggcgaag ctggcgtcga agcagcacag gctggtcgag ggctcgcgcg agcgggtcgc
    16921 gatgctccag tagccggccg cggcgttgtg caccgagttc atgaatttcg tgggcgacac
    16981 ttcgcgctcg gcggtggcca gcgtctcgca catctcgtgc aggatctcgc cgtcgctcga
    17041 cgaggaggtg aagacggttg gcgtgactgc cgcatcgtgt ccggcggccg cgaaggcctc
    17101 gcggcccact gccagcgcga gcttgaccgg gatgccggcg cggcgccgct cggccggcgg
    17161 cagcagctcg ctcgggggaa tcgccgtgcg cgcggggcga tagggctcgg cgcccgcgag
    17221 caccgcgcga gcggccggcc agccgctcag gccggggccg aggacgccga tgccttcaat
    17281 gaacgcgcgc atcgccgatg acgaggctgc agttgctgcc gccgaagccg aaggaattgg
    17341 tgagcacgcg ccgcaggcgc gcctcccggg tctcgagaac gtagttgctc ttcagcgcag
    17401 ggtcgacgtt gcgggtgtgg gcgctgcccg gaaggaggcc gtgccgcagg gccagcgtcg
    17461 aaatgatcgc ctcggtgatc ccggcggcgc ccagcaggtg cccggtagcg cccttggtcg
    17521 agctggcggg cgttttccga ccgaacagat cgacgacggc cgtgtcctcg gcggcgtcgt
    17581 tcgagggcgt cgccgtgccg tggagattga tgtagtcgat gtcggccgca ccgagccgcg
    17641 ccgaggcgag cgcgcgctgc atggccagct tcgcgcccgc gccctcgggg tgcggcgccg
    17701 acatgtggta cgcatcggag ctttcgccca cgccgaggag tatcggggcg tcggcgctcg
    17761 cgccggcatg cggcttctcg agcagggcaa agcccgcgcc ttctccgagc gagatgccat
    17821 cgcgctcgcg gtcgaacggc cggcacgggc ggcgcgaagt gagctcgagg gagcgaaagc
    17881 cgtacagcgt cgtgaggcac agcgaatccg cgccgccgac caccgcggca tcgcaggcgc
    17941 cggccgcgat catgcgcgcg gcgttgccga agaccttggc ggtggaggag caagcggagg
    18001 agacgacgaa cgaaggtccc gccaggccga gatagcgccc gacgaaatcg ccgagcgagt
    18061 aggtgttggc ggtcgcgtgg tagcggaagc cgcgcggcag ggacccgctc gccggatcgc
    18121 gccgctggta ggcgagctcg gcctcgagga tgccggaggt gctggtgccg atgaacacac
    18181 cgatgcggct cgcgccatag cggccgcgcg ccgccgctac ggcagcggcg aagccgtccg
    18241 cctcgagggc catctgcgca atgcggttgc tgcggcagtc gtaagcttcg aggccgcgcc
    18301 ggatgcccgc gtccttgaga ccaggcacgc ttccgaccca ggtgtcgatc gaagtagtct
    18361 cgaattcgca aggcgtgagc ccggagcgct tcgtgcgcag cgcatcgagc accgccgcgc
    18421 tcccggtgcc gaggcagttg acgatggaga acctcgtgat caggagaggc tgcatgccgc
    18481 gggaagggaa acgcacttga ggccgcgcgc ggcgagctgc tcgagcaggg cgggcaggac
    18541 ggcgagcgaa agcggctcgc tgtcgtgcag caggagcacg tcgcccgccg cgagcccggc
    18601 ggtgaggcgc ccgagcaccc tggcggggtc gcgcgcaacc gtgtccaggc cgcggcgcgt
    18661 ccacgagacg tagcgcagtc ccatcggcgg gaggacgagg ccgaggaacg ggctgcggaa
    18721 gccggccggc gcgcggaaga actgcggcgc ttgcccggtg atggcggcga cggtggcctg
    18781 cgcgcgctcg acctcgcgcc gcaggaggga gatgccgtag aacgcgaagg cgtgagaatg
    18841 gcggtcgctg tggttctcga cgctgtggcc gcggcgcacg atctcgcgca cgatctccgg
    18901 atgcgcccgc gccctttccc cgacgcagaa aaagctcgcc ttggcctggt agcgctcgag
    18961 gagctcgagg acgcgaggtg tcagctccgg atgcgggccg tcgtcgaacg tcaggcacac
    19021 gtgccggtgc gcggcggcgg cggccgggag ccggctcagg ttcggcccga gcaggcggcc
    19081 gcgcacgtga aacactccgg cggcgatcac gagatgattg gcgatgagcg ccgcgccaac
    19141 ccagggccac atcccgggcg cgagcgcgag gacgatcacg ccggctgcgt gcaccaggat
    19201 ggaaacgcgg atcacgggcg tcatgccggc ttcgggaaga gcggcttttc aagcacggcc
    19261 cgccacaatt gaatccaggt cggccaatcg tgcgcgcccg gcatcctgat cacgcgctcg
    19321 gccggcagct ggcgggcaag catctcgctc gccgccgcgt agcggtcgcc ggtaccgaag
    19381 ccgagatagg cggcgggaaa ccactttgcg tcggccggtt cgagcgcgtc ggggccgcgc
    19441 gagccgagaa acggcgcgag caggatcatc gcctcgagct cggccgcgcg ccggcgcgca
    19501 agagagatgc agccatagcc gccgagcgag atccccatga gccagatgcg agcgtagccg
    19561 ttggcgcgca tcggcgcgat gacgtctgcc gcaaggcgct cgccgacgct gccctcgagg
    19621 tagtagtccg cgtcggcatc catgaccgcg acatcgagcg gcgatccccg ctcgcgaagc
    19681 gcggccacga agccccggtc caccaggtct tgcgcgcaag ccttggcggg cggcagcatg
    19741 acgagcagat tgccgctcgc gcccggcgcg cggtcatgaa gcgcgcgcat aggccccttc
    19801 ggcgccggat ggcctgctca ggatcgctga gaacaccagc gccatcgccg cgccgaggcc
    19861 cacggtggag ccgatggcgt tcagcaccgg cacggaggag aacgcgagca ggccgaaccc
    19921 gatcacggtg gtgacggcgg cgaacacgag ggacaggatg gtgcggccgc ggtcctggac
    19981 aggcgccgcg cgatcgaaga acagcgaata attcgacccg accgccacca cgagcagcag
    20041 cccgacgagg tggaaaatgg aaagctcgtg gccgcccagc gcgagcagcc cgcaggtcac
    20101 gagcgccgcg gcggcgagcg gcgccagcac gtcgaacacg cgccgcggcg agcgcagcgc
    20161 agcgagcagg agcgcgacga tcgcggcggc gcccagcagc gaattgatct gcgcctcgcg
    20221 cagatagccg cgatagagct cgtccaattc ctgccgcagg tcgagaagca cgccggccga
    20281 agttcccgcc acggcgccgg cgatggcctt ggcgtcactc accccgcgca gcggcagcat
    20341 cgccgcccag ccagtgggcc gcttcacgag cagcgcatcc agcctgagcg cgaggctcgt
    20401 accctgcagg ctcgcacggt cgagcagcgg catggtgcgc gcggcgccga cgtcgcgcac
    20461 gaagggctcg aacacgttcg ggcggaaggc gagccctttc tgagcctcct gcaggttttg
    20521 ccgcagcgtc ggcggcggcg gtagcgccgc ctggcgctcg cgttgcgccg cttcgctcgg
    20581 caggtaggcg gcgggcgaat cgaagccctg cagccagcct ttggccaccg catcgcgcaa
    20641 tgccagcgcg gcccgctcgg ctgcctgcag cgcggcctgc tcgtccgcgg cgtgcacgac
    20701 gacgagatag cgcgggtccg gggcgccgag gtcctggcgc agctcgcggt cgagcgcttg
    20761 ctcgcgcggc gagatcgggc tcaagctcgc agccgagccg ctccacaggg gctgcgggcg
    20821 cagcaggagg aatgcggctg cggccgcgat caagacaacc agcggatagc gcagccgggg
    20881 cgccgcgcgc gcgagcgcca tgaccctcgg cgcgaatccc gctacggcgg cgacggcgaa
    20941 tccgcgcggc agccacgcag gcagggccca ccgcgtaacg gcggcggcga cgacgagccc
    21001 ggcaatggag aacaggccga gctgcgcaag accggtgaag cctgaaaaga gcattgcgct
    21061 gaacccgcag atcgacgtag ccagcccgag ccgcaacgtc ggccagatgc gctcgaaagt
    21121 gctctgcgcc gtcgcgccgg gcgcgttctg cgtgaagaga tagatcgcgt agtccacggc
    21181 ctcgccgatc agcgtcacgc cgaaagcgag cgtgatgccg tgcaccgagc cgaacgcgag
    21241 gctgacggcg gcgacgccgg cgagcgcgcc gctcgccacc ggcagcagcc cgaggccgag
    21301 gacgcgcacc gagcggtaaa gcgcgatcag catggcggcg atgagcaccg tcgcgatgag
    21361 cgagaaccgc cacgcgtcgg cgcggatctt gtcgcggctc gcgaccgaaa agacgcccgg
    21421 cccggtcacc tgcagccggg cgccggcgcc ggcggccgcg gccgcgaacg aatcctggat
    21481 cagtcccagc gcgcgctttt gcgcatcgat gtccgaggcc gcggcgcgtg tctgcgcgac
    21541 gaggagcgcg cggcggctat cctccgagaa ccatacgccc ccgtgcagcc gcgggcgcgt
    21601 cgccggcgcg agctcttcga gcaggcgcag cagctcgccg ccgggatccg aagccgacag
    21661 tcgctgcaac agcacgcccg caggcgacgc gagttgtgcg agaagctctt ccaggctcgc
    21721 ccgcaggccc gccgccgaga agcgctcggc cttgaccgcg gggctaagaa ggtagcggtg
    21781 gcgccagacg agatcgcggt ccttctcgaa gcccgcctcc tcgccgttgt tcaccgcggc
    21841 gaattcgcgc gaggcgcgca gtgaagccgc gaggcgcttg ctgacctccg cgagccgatc
    21901 gggggagtcc ccctgcaccc cgatcagggt cagccgcgac accacgccgt cgcgcagctg
    21961 ctcgaccagc gcctgctgga cgggggaggg cgatcgcggc agaaaggccg acaggtcggt
    22021 gctgaactcg gtgcgcgcga tgaccgcggc gcacgccgcg gcgcctgcga tccacgccgc
    22081 aagcgcccag cgcgcggccg ggctcacccg gcctcccggg taacctcggt caccgagcgg
    22141 tcgccgccgg cttcgtcgat ctcgatccgg ttcacccagg tgccgctgcc gccgacgcgg
    22201 atctcgctcg ccgcctgccg catgcgcggc tccttcggcc tgagccgcag gcgccacgcg
    22261 cccgcgtcac cctccaggcg cacttcgtag aatcgctgca aggtcgcgag gtccccgttg
    22321 agcgtggaac gcatgctttc cacgaaggcc cacagtaccg ggtgctcctg cagcgggaaa
    22381 gtgcggcgct gtcccttgtc cttgcgctcg acgatcagtc ggtcgccctc caggatcagg
    22441 ctctcctgcc gtggcgacgt catgtgcttc tcgagcctgc ccggcgcgac gtaaaccagg
    22501 gtgcccgagg attcgagcgg cgcgttgagc atcgcgaagt gcttgcgctc gacgaacctg
    22561 ccgcgcgcgc tcttgacctg cgcgaggtct ttcatcagcc gctcgagcac gagcgtcggc
    22621 ttgtcctgcg cttgggcgag cgcgggcgca aggcacccca gggccaggac cacagccgcg
    22681 agcctatgcc cagtagtcat agaagttgaa ccagttgtac ggggcctcgc ggcagtggtg
    22741 ctccaggcgc ttcgcgtagc gctcgaccca gggccgcacc gactcttcgc ggttgcgccg
    22801 ctcgagcgga attgcatcgg cgaaggtctc caagtggatc tcgtaacgcc ggccgccacg
    22861 gtagatgcca aagaacagga ccaccggcgc ctgcaccagc cccgcgagca gcagcgggcc
    22921 gagcggaaag cgggcttcgc cgcccaggaa ctcgcagcgg taggtgcgct cgctcctcca
    22981 tgcccggtcg ccgagtatgc cgacgatctc tccgcgcgcg aggcattcgt gcacgcgcag
    23041 cagcgcgtcc gggctgccga gcgggattac gcgcgcctcg gcgcccggcg caagcgggcg
    23101 cagcacgctg ttcagcttcc cggcgttgtc cacgtacatc aggatgttga tcggcggcac
    23161 gccttcgccg ggtccgcacg cccgcagcac ctcgaagctg ccgaggtgcg atccgagaag
    23221 gatgcagccg cggccgtcgc gcagcagcgc ctgcgtttgc aagccagcgt ggatttgcac
    23281 gtcgtaggaa gagatcctgc ccgccaggaa atagacgcga tcctggatgg tcgaggcgaa
    23341 ggtgtagtag tggcgaaaca cgtcgccgag cgtcgcgcgc cggccgagaa cgcgccgcag
    23401 gtagcctgcc gaagcgctgc gcgcgtgcga cgagaacaga acgaagtagg cgcagatcgg
    23461 aaccagcagg atccggccga gcggtcggcc gagcgccagc atcagccaga ccgccaggcg
    23521 catcgcgagc acgctgccgc gctcggggtg ctcgacccag tccggaggcg cagctactcg
    23581 gccgggctgg cggagcattc gacttcgccc acgagcacgg tgttgccctt taccgcgcac
    23641 tcgaacttga tggagcccaa gccgtcgctg aactcgatga gaacctcgtc gccgggccgc
    23701 gcgggagagg gaaacttggc gcgccggatg cgatacggcg cgaggcttac gccgaggtcg
    23761 gcttcgatca gccgcaccgc ctcgcttagc aggaccgcgc ccgggatgac cgggttgccg
    23821 ggaaagtggc cttgcgcggc gggatgctca gcgctcagcc gctgccgaat tgtcgccacg
    23881 tcagccgact ttcgccacga ggctgtcgag ctcggcgcgc ggcagctttc ccgtctccgt
    23941 gcgcggcagc gactccacga tgcacagcgg ccgcggaagg aacgcgggat cgatacgctg
    24001 ccgcagcgcc gccaggatgg tctcggcggt gagccccggg gcgaccgcga aagcggccag
    24061 ccgggtgacg ccgcccgggt cgtcggctgg cacgacgaac gcgccgtcgc gcacgccggg
    24121 aatcgaggtc aggtgatggt tgaggctggc gagcgaggtg cgcttgccgg cgacgttgac
    24181 gagatccgcg gtcctgccat gcagcaaaaa gcggccgcgg gtctgcagct cgatgacgtc
    24241 gctcagcagg aactccatgg gcgcctgccc gcccttcacc caggtgcctt tctcgtcctg
    24301 gcgcatcgtg aggtggggga agagctgcca ttcggccgat tcgctcgggc gccgggtcgc
    24361 caccagtccc gcctccgtgc agccgtaaat ctcgtgcagc ggagcgccca ggcgcgcctc
    24421 ggcttcgaca gcgagcggcg tcggcagcgg cgcggtggag gacaggagga aatccagtga
    24481 cggcagggcg gttgcgtccg ccagcagcgc acgcaggtgt accggcgtgg tcaccaggcc
    24541 gcgcggcctt ggcagcgcct cgagctcggc gcagatatcc gccggaaaaa acgggcgccc
    24601 gccatgcagc acgagcgcgc cctggagcgg caggagcacc gtggattcca ggccgtacag
    24661 gtgctgcggc ggcacggtgc cgaggatcgc catgcccggg aagtcgcgga tgcgcaggcg
    24721 ctcgatttcg gcgatcgccg tcgccaccag agcgccccag gacttgggat gcggcacggg
    24781 atcgccggtc gagccggagg taaatacgat ggcggcgatg cgctcctccg gtatcgccgg
    24841 gaccgcgggc gacgggacgc cgcgtccacc gtcggccgct gcgaagtcgt cgtcgaggca
    24901 atagagatcc gggtagtcgc gaaccagcct ctcgatcacg tcgggcgtgt gggtgggcgg
    24961 catgaggctg atctggccgc gcaccagcgc cgccgcaaag ccggcggtga agcgaaaacg
    25021 gtccgtgcag aggttgatga cgtatcggcg atccggaagc gcctcggcga ggcgcgcgac
    25081 ctcggcgaga aattcgccgg cgcgcacgag ctcgccttgc cgctgcgcga aaagcgcatc
    25141 cggatcgtgg cgaacgaggg gctgagcggc catcggcgac gcctcagcgc gacagcaagc
    25201 tcgacgcgcg gtccttcgcg taagcctgta tcgcggtggc aatcgacgac tgcggcaggt
    25261 ggcggtagcg gatcacgcgg tacaggtagt cgagcgcgaa catcagcccg acgagcggga
    25321 agctgagcac gttgatgaac aacgaccagc tctcgagcga tccataggcg aggagaagcg
    25381 cggagcccgc gagctgcgcc gcgaagaaca cgcaccacgc ggccgtgagc ctgcgcgtgt
    25441 agcgttccat ggccggcggc aacgtagcgc cgtgcgcttg gcgcgcgagg cgcgccaagc
    25501 gggtaatgat ggcttcccgc ccggggagca gcgtgcggcc aaacagccac agcatgaagg
    25561 aatacgccgc ggcgtgaggc acgccgtaga gcaccgtggt tcccagcccc gtgccgtgcg
    25621 cgagcagcca cgtggcgccg gcggcgccgg cgacgatgag gagccacagc atccgcttct
    25681 gcgagcgcgc gatgagccag caacccagcg ccgccatcgg cgccatcacc aggagggttc
    25741 gcgccggatc gccggcgctg ccggcgagcc cgacatggat caggatcggg tagccgacga
    25801 tggcggcggc ggccagcacc ttccaggcga tggcggccgg gcgcgccatt tcagggcgcc
    25861 cggtgcgcct ggatgtgctg gctgagactg cgcagggaac tgaagatgct tgcgttgttc
    25921 ttgttgtcgg accgcagctt gacgccgtag gccttggaga tcgcgagcgc gatctccagc
    25981 acgtcgatcg agtcgaggcc cagcccgtcg ccgtacaagg gcgcggtcgg gctgacttcc
    26041 gccggctcca tttccagctt gagggtggcg acgatgacgc gggccaattc cagctcttcc
    26101 gcggtcagcg cgtccgtctg cggagcaatc tctgtggtga cgatgctcat gtttcgctcc
    26161 aatggccgtt gggctctttg atcggattat acgggcgatt cagcgggcga ttcagagtgc
    26221 gcctacaata ccaaagccaa agcagatcat atggttaatc accgctgcga cgtgctggtg
    26281 atcggcgcgg gtcctgccgg gtcgaccgtg gctgcgctgc tcgccgaacg cgggcgcgag
    26341 gtcgtgatcc tggaaaaaga gcgccacccg cgctttcaca tcggcgagtc gctgctgccg
    26401 ctcaacctgc cgctgttcga gcggctgggg gtcgccgcgg agatccagcg catcggcatg
    26461 ccgaagctcg gcgcggagtt cgtctctccc tggcacaagc agccggtgac cttcgacttt
    26521 gcccacgcgc tggacaaatc ctttccttcc gcttaccagg tgcgccgctc cgaattcgat
    26581 gagatcctct tcaagaacgc ggtgcgcaag ggggcacgcg cgctcgaggc ctgccgcgtg
    26641 acgaccgtgg aattcacgcc gcagggcgcc gcggtggctg cgcggctgga agacggagcc
    26701 gagcagcgct gggacacccg gttcgtagtg gacgcgtccg gccgcgacac ttttctcgcc
    26761 agcaagatgg ggatcaagcg gcgcaaccgg aagcacaaca gcgcggccat ctacggtcac
    26821 ttcaccggcg cgaagcggct gccgggcaag gccgaaggaa acctgaccgt gttctggttc
    26881 gagcatggct ggcagtggtt cattccgctt gccgacggca cgaccagcgt cggcgcggtg
    26941 tgctggccgt actacatgaa gacgcgcaag accggcaccg accagttctt cctcgacacg
    27001 attgcgctgt gccccgcgct cgcggaacgg ctgcgcgacg cgcagctcac ggcgccggtc
    27061 accgccaccg gcaactactc ctacgttgcc gagcgcacca tgggccgaaa ctacgtgatg
    27121 ctgggcgatg cctatacctt catcgacccg gtgttctcca ccggcgtgct gctcgcgatg
    27181 caaagcgcct tcgccggcgc ggacaccatc gaaacctgcc tcgaccatcc ggcgaaggcc
    27241 ggcgccgcgc ttcggcgctt cgacgcgatg atgcggcgcg gccccggggt cttctcctgg
    27301 ctcatctacc gggtcacgac gccggcattg cgggagctgt tcatgaagcc gtccaaccgg
    27361 ttccgcgtgc aggagtcgct gatttccgtg ctggccggcg atatctaccg cgacacgccg
    27421 gtgcgcgcga ggatgcttct gttccgtgcc atctactacc tgaagagcct gtccctgctg
    27481 cgccagagcg tgtcggcctg gctcaggcgc cggcgcgcga ttcagtcagc gtagccacga
    27541 tcgcctgaag tagacagtgg ccacataagt gtgcgaggat gtggtcaatg gcaacatatc
    27601 atcacggcga tctgaggaca gcggtgctgg ccgcggccgg caagctgatc gagaaagagg
    27661 ggctcggcgg gctcagcgtc cgcgaagccg cgcggcgggc gggggtgtcg cataacgccc
    27721 cttaccgcca cttcccggac cgcgacgcgc tcctcgccgc gctcgcggcg caagggttca
    27781 gccagctggg gaaggcgctg gaaggccgtt ccggacgcga gctcggcgaa gcctatgtgc
    27841 gcttcgccct ggggcatccg cagcggttcc ggctcatgtt cggcgggcag ctcgcgctcg
    27901 accggtaccc ggaattacgc acgcaggccg aggcgacgca cgggcgcctg acggcggcgt
    27961 tcgccggact gggcgccgaa gcgaagttcg ctgccgcagc cgcctggtcg ctggtgcacg
    28021 gcctcgcgca gttgattctc gacgggcact tcgagaacga tgagggtttc gtgacgaaag
    28081 tgatcggcgc gatccgcttc gccgtgggac ctcagcgctc ggcgtaggag gcgaaggcct
    28141 cagcgcacgt ggcaagctcc gcatggcagc gcagcaggcg gaaagccggc cgcgcggcgc
    28201 gcgtgcccga gccgtaggtc ttccagagct cgagcgcttt ccccttgtcg ccgctcgcga
    28261 cgtagccggt cagcgccacc agcaagaggt attcgcgcgc atcggcgatc agcggctgct
    28321 cgccggcgat gagagggccg gcgagctcgg ccatgcgcgt cgcgttgcga gcccccaccg
    28381 cctggaacaa cgcaatccag cgccgctgga actcctgcaa gctcgaatag cacgcgcttc
    28441 ccatcatgcg cgcccagacc ggcgcgagat cgctcggcgc caggtaggga ttgagggctt
    28501 tggcaacgcg cagggcgctg tgcaaccaga tatcgagctc gcgcgggtca cggcactcga
    28561 tcaggcgcag cttgacgagc tcgaggtcct tttgcagctc ccgcggcacc gactcgggca
    28621 ccggcgggcg aggccgcagc aggaagtcgc gcgcgtacca cgccaggcgt gtgttctcga
    28681 cccgctcgaa cgcatacgcg cccttgaaga gcgggttgat cgcgcgccgg ctgcgatcgc
    28741 gctcgatcag ctcgagcagc ggcagctctg cgttgaggag cgctacgacg tcggcggcgc
    28801 tcttgtccat gaagcgatgg cgcgccgcgt tgagatcgag caccggcgcg tagtccgagt
    28861 tcgccggcat gccgtagctc gcgaacagcg gctcgagcgt ggcgcggctg ccgagatagc
    28921 gcgcgtcgag gtcgccggct gtgaggacgt gcaccgtcca gagctccttc gccacgcccg
    28981 ggtgctcgaa cacgcccgcc tgggcctcga gcggcaccgg cgcgtcgccg gccatgatca
    29041 gcaggtcgta gtcgctcgcc gcgaacaccg cgtagtgcgg gaactccgcg ccgagcgcgc
    29101 cgagcacggt ggcgacgagc gaagcgtcca tctcgtagag ctggatccac tgcaccagca
    29161 gcccgccggg gttgaggtgc ttgcggatgc ggcggtagaa ctcgcgcgtg aataggctcg
    29221 acacgccgct cacccagggg ttggagggct ccgagatgat caggtcgtag cggcggttgc
    29281 gcgtcgagaa gaagctcttg gcgtcgtcga tcaggatcgc gccgcgcggg tcggcgaacg
    29341 cgccgctgtt gcgcggcatg aagccgcgcg aggcctcggc catcgccgac tcgatctcga
    29401 cggtctcgac gcgctcgatg tccaggctct gcagcagggt gtgggtggtg agcccggtgc
    29461 cgatgccgat caccgccgcg ctcttcgtgc ccggcttgag cgcgagcggc agcgcggcgg
    29521 tgagcaccat cgtgatctcg tcggtgccgc gctcgccggc cgggttcagg ttgatcgagc
    29581 cgtcggactt gccgttggtg cgcaggctcg tggcgtcgga gtacctcacc aggtgcaccg
    29641 tggcggtctt gccgtccttg ttgaagagga tcgtggcgtc ctgcgaggtc tggagctcgc
    29701 cgtggcggaa gaccccggcg gtcatcttgt gcacgtcgag ctccactccg attcctaccg
    29761 cgaggaacag cacgccgcag acaacagcaa cctggatgta ggttcttttc acaaactgaa
    29821 gaagaaccag gcccagtgcc acatcgacga gtgcccccgc gatcagcgtc cccttcaagc
    29881 cgagcagcgg cagccccgcg tgcaccgcca gcacgacacc ggcgatcgcg ccgagggtgt
    29941 tggcggcgta cacctggccg atcgccgcct cgcccgcgcc gcggcgcagg agcgcggcgg
    30001 tgatgagcgg cagcgtcatg ccggcgcaga aggtggccgg cagcatgacc atcgcggcga
    30061 tcgcggcgcc cgaaagattg aacagcaggt agccggtctc gctgcgcgcc aggcccttca
    30121 gcagcacctc catgagcgag aacgtgaagt cgtacaccgg cagcgtcgcg agcgcagcga
    30181 ggcccatcgc gacctgcacc ccggcgagca agcgcaccgg gtcggcggcg gcatcggcgc
    30241 gccgcctcac ggcgaggccg ccgagggcga ggcccaagat gaaggtggcg agcatcagct
    30301 cgaatgaatg cgtcgaggcg ccgaggacga gcgagagcat gcggatccag ccgatctcgt
    30361 agacgaacga cgccaggccg gtgaagaaag cgatcgcgag gagcaggcca agattgcttt
    30421 ctgaaacaga aactctcttg aggggttcgt ggcgggcagg ccgcgcgaga agccatacca
    30481 ccccggcgat cacgagattg agaacgcccg cggtccgcag cgtgccgggg aggcccgccc
    30541 aggcgatcag cacgaagccg ctcgcaagca cgcccgcggc cgcgccgagg ctgttggtga
    30601 aatacagcat cgccaccgac tcgccggccg cggcgcgatg cgcgcgcacc agcccagcgc
    30661 tcatcagcgg gaaggtcatg ccgagcagca ccgactgcgg caggatgagg agcgccgaca
    30721 cggcgagctt cgcgccgagg gcgagccact cgttgcccag cccgggcagc aggcggtcgt
    30781 acgaccaatc ggtcaccgcg acgaacaccg gatgaaaaac gagagccgcg atgccgacgg
    30841 ccgcctccac gatcgcgtag gcggcgagcg ggttcgcgag gcgggccgag cggcggccgc
    30901 accaggctgc gccggcggcc attccgccca tgaatacggc gagcaccagc gactgcgcgt
    30961 acgccgcgtg gccgagaaag agcttcaggt agtgcgtcca caccgactcg tagatcaggc
    31021 cggcgaagcc cgaagccgtg aacaggacgt agaaggcgcc gcgcgacatc gggagcgatg
    31081 ctagtttaag aaatggggtt tcgtctttcc gcgttctact ttgcgttttt cgcgtatgcc
    31141 ggcgtgtacg tggcgtattt cccgctttac ctcgccggcc gcggcttgag cgcggtggag
    31201 atcgcctggg tgctcgcgct gccgccgctc gtccgcatcg tcgcgcccgg cgcgtggggc
    31261 tggcttgccg accggaccgg cgcgcaccgc gcgatcgtcg cattctccag cgcagtgacc
    31321 gcgcttgcgt tcgcgctcct gccctttacg gagaatgttg ccctcctgat cgcgctgatg
    31381 agcgtgttct ccgcgggggc gctgccgatc gtcgaggcga tcacgctcgg ctcgctcgcc
    31441 gggcaatcgg gtcgctacgg gccgattcgg ctctggggct cggtaggctt catcgccgtg
    31501 gtgctcgccg gcggggtgtg gctggacgcc cagccggtat cgacgctgcc ggcggtgctc
    31561 gtcttgttcg ccctctttac gctggccatg gcgctcgcgc tgccttcgat caagccgctc
    31621 gggccatccc gagcggccac tttccgggtc agccctgaag tgcgcgcgct ctttgccgcc
    31681 ggcttctgca acgcggttgc gcacggcgcg ttgtatgcct tcctgagcct gcacctcgag
    31741 gcgctcggct acagcgcgac gaccattggc atgctctgga ccgccggcgt gctcgccgag
    31801 atcgtggtgt ttctctacct gccggcgctt ttccggcgat tcgccctttc gagcgtgctg
    31861 gtcgcgagcc tgctcctggg ggtcctgcgc ttcgccgcca tcgggtgggg cgccgggcag
    31921 gcgtggatag tcctgttggc gcagctgctg catgccgcaa ccttcggctc gttccacgcg
    31981 gccagcgtcg ccgcggtgca gcgcgtgttc cccgagggcg cgcaagcccg cggccagaca
    32041 ctcttttccg gtttcaccta cggcgccggc gccgccgcag ggctggtgct ggccggctgg
    32101 gcatgggccg gaggaggggc gccgctcgcc ttttcggtga gcgcaatggc ggcggctgcg
    32161 ggcgcattct ttgctgccag gctgaaacgg gccgggctgt gagctgtcaa acagaacagt
    32221 ctgccaaacg ttgagaggtc aacatgattc gtgcaattgt cgttgccgcc gctgccgcag
    32281 cggcagtcct ttccggctgt cagaccgcgg agacaacgca ggccggcgtg gtcggcgtcg
    32341 accgcgatca acgcttcctg gtctcggccg acgaggtcaa cgccggagcg cagaaggcat
    32401 acgcgcagat gatggccgaa gcgcagaaga agggcgtgct ggacaaggag ccggcgcagg
    32461 tgcaacgcgt gcaggcgatc accaagcgcc tcgttgcgca ggccggtgcg ttcaggcccg
    32521 acgcggcgaa gtggccctgg gaagttcacg tcatttccct ggacgaggtg aacgcatggt
    32581 gcatgccggc cggaaagatg gcgatgtaca ccggcttgat ccagaagctc aacgcgaccg
    32641 atgacgaaat tgccgcggtc atgggccacg agatctcgca cgcgctgcgg gagcactcgc
    32701 gcgagcagat ctcccggcaa atgggaacgc agatgggcat cggcctggtc ggcgcgctgt
    32761 tcggcatcgg cgagctcggg cagagtgtcg ccggacaggt ggccaacgtg acgctcaacc
    32821 tgccgaattc gcgccaggcc gagaccgagg ccgatcgcat cggcatcgag cttgccgccc
    32881 gggcggggtt cgatccgaac gcggcggtca gcctctggga gaagatggcg aagctgggcg
    32941 gcagccagcc gccgaagtgg ctctcgacgc atccgccgca cgaagaccgc atcaacgacc
    33001 tgcggaacta tgcgcagaag gtgatgccgc tctacaccgc tgccaagagc ggcgcaggcg
    33061 ccggcaaata atctaaaatc ccatgcttgg gaaagacgct ctacgacaag ctttgggaaa
    33121 gccacgtcgt ccacgtcgag gacgacggga cggcgctgct ctacatcgac cgccacctcg
    33181 tccatgaagt gacgagcgcg caggcctatg agggcctgcg cggcgccggt cgcaagccct
    33241 ggcgcatcga atcggtggtc gccaccgccg atcacaacac gccgaccacc gactggaacc
    33301 agggtatcgc gggcgtgcgc gacccgatgg cccggctgca gatcgaaacc ctcgacgcca
    33361 acatgaagga attcggcgcc aaggtgtatt tcccgtacct cgacaagcgc cagggcatcg
    33421 tccacgtcat cgggccggag cagggcgcga ccctgccggg gatgacggtc gtctgcggcg
    33481 actcgcatac gagcacgcac ggcgctttcg gctgtctcgc ccacggcatc ggcacctccg
    33541 aagtcgagca cgtgctggcc acgcagtgcc tggtcacgcg caagatgaag aacatgcgcg
    33601 tgaacgtcga aggtccgctc gcgcgcggcg tgacggcgaa ggacgtggtg ctcgcgatca
    33661 tcggccgcat cggaatcgcc ggcggcaccg gccacgccgt cgagttcgcc ggcagcacca
    33721 tccgcgcgct gtcgatggaa ggccgcatga cggtgtgcaa catggcgatc gaggccggcg
    33781 cgcgcgccgg catggtcgcg gtcgacgaaa ccacgctcga gtacatcaag gggcgcaagt
    33841 tcgcgccgca gggctccgac tgggagcgcg cggccggcta ctggcgcacg ctgaagagcg
    33901 atccggacgc gaaattcgac cgtgaagtca cgctcgacgc gcgcgagatc cggccccacg
    33961 tcacctgggg cacgtcgccc gagatggtgg tgtccgtgga ggaccgcgtg cccgacccgg
    34021 acaaggagaa ggacagcctg cgccgcgaag gcatggagcg ggcgctcgag tacatgggcc
    34081 tcaagccgcg cacgccgatc accgacatcc ggatcgacaa ggtgttcatc ggctcatgca
    34141 ccaactcgcg catcgaggac ctgcgcgccg ccgctgccgt cgtcaagggc aaacgaatcg
    34201 catcgaatat tcgtgcggca ctggtcgttc ccggctcggg cctggtgaag cagcaggccg
    34261 aacgcgaggg cctcgaccgc atcttccgcg acgcgggctt cgagtggcgc gagcccggct
    34321 gctcgatgtg cctgggcatg aaccccgatc ggctcgatcc cggcgagcgc tgcgcctcga
    34381 cttcgaatcg caatttcgag gggcggcagg gcacgggagg ccgcacccac ctcgtcagcc
    34441 cggccatggc ggccgctgcc gcgatcgccg ggcatttcgt ggacatcaga aaatggtgaa
    34501 gttcaccgtg ctgaatggcg tggtgatgcc gctcgaccgc gccaacgtcg acaccgacca
    34561 gatcattccg aagcaatatc tggcgtcgat caagcgcacg ggctatgccc aaggactgtt
    34621 cgcggcatgg cgcaaggacc cgcaattcgt gctcaaccag ccgctctaca agggcgcgag
    34681 catcctcctc gcgcgcaaga atttcggctg cggctcgtcg cgcgagcacg cgccctgggc
    34741 gctcctggat tacggctttc gctgcgtcat cgcgccctcg ttcgcggaca tcttcttcac
    34801 gaactcgttc aagaacggct tgctgccggt gatcctctcc gaagccgagg tcgatcgcct
    34861 gttccacgag tgcgctgcgt tcccgggctt ccgcctcgtg gtcgacctgg agcagcaatc
    34921 ggtttccacg acgaacaacg aaaaatcgtt ccgtttcgag atcgaccctt tccgcaagta
    34981 ctgcctgctg aacggcctcg acgagatcgg cctcacgctg cgccacgcgg acgcgatccg
    35041 cgccttcgag gcgcagcgca aggcgcagta tccctggtac ttctaggatg aaaatcgcgg
    35101 ttctgcccgg tgacggcatc gggccggaga tcattgcgca ggcgcgcaag gttcttcaaa
    35161 agctggattt gtcgtttgaa ttcgcggagg cgccggtcgg cggggcgggc tacgaggcgg
    35221 cgggcgatcc gctccccgca gccaccctcc agctggcaag agccgccgac gcggtgctgt
    35281 tcggcgcggt cggcgacccg cgctacgaca agctcgagcg cgccaagcgc cccgagcagg
    35341 cgatcctcgg gctgcgcaag gagctcgggc tgttcgccaa cctgcgcccg gcgcgcgtgc
    35401 atcccgagct cgcggaagct tccgcgctga agcccgaggt cgtgtcgggc ctcgacatcc
    35461 tgatcgtgcg cgagctcacc ggggatatct atttcggcaa gccgcgcggc atccgccagg
    35521 tcgagggaat tcgcgagggc ttcgacacca tgcactacaa cgagggcgag atccggcgca
    35581 tcgcccgcgt tgctttcgag gccgcgcgca agaggaacaa gcgcgtctgc tcggtcgaca
    35641 aggccaacgt tctcgagact tcacagttgt ggcgcgaagt cgtgcaacag gaggcgaaaa
    35701 gctacggcga tgtcgagctg actcacatgt acgtggacaa ctgcgcgatg cagctcgtgc
    35761 gcaatcccaa gcagttcgac gtcatcgtca ccggcaacct gttcggcgac atcctgtccg
    35821 acgaggcctc gatgctgacg ggctccatcg gcatgctgcc ctcggcatcg ctcgacgagc
    35881 gtggcaaggg cctctacgag ccgatccacg gcactgcgcc cgacatcgcg ggcaagggcc
    35941 tcgccaatcc tttggcgacc atcctgtcgg cggcgatgat gctgcgctac tcgctgcgcc
    36001 agccgcaggc tgcgcagcgc gtcgaacacg ctgtgacaaa ggtcctcaag tcgggactaa
    36061 ggaccgccga catccacacg cctggagcgc gccgcgtcgg cacccaagaa atgggcgacg
    36121 cggtcgtggc cgcgctttaa actatgagca tgctgagagt cggaatcgtc ggctggcgcg
    36181 gaatggtggg ctcggtgctc gtgcagcgca tgcgcgagga gcgcgacttc gaccatgtcg
    36241 agccggtgtt cttctccacg tcgcaggcgg gaggcaaggg gccggagatc gggaaagcga
    36301 ccgaggcggt aaagaacgcg accgaccttg cggaactgaa gaagctgccg gtgatcatct
    36361 cgtgccaggg cggcgactac accagcgaga tctatccgaa gctgcgccag cagggctgga
    36421 aaggctactg gatcgacgcc gcgagcgcgc tgcgcatgaa cgacgacgcc gtgatcatcc
    36481 tcgacccgct caacatggat ctcatcaaga gcgcgatcaa ggcggggacg aagaattaca
    36541 tcggcggaaa ttgcaccgtg agcctgatgc tcatgggcat ggcgggcctg ttccagcgcg
    36601 acgaggtcga gtggatgacg agcatgacct accaggcggc ctcgggtcag ggcgcggcgg
    36661 cgatgcgcga cctggtggcg cagatggcga cgattgccga tcgctcgcag caactgctcg
    36721 acgacccggc ctcggccatc ctggagatcg accgcaacgt cacggagtcg atccgcgccc
    36781 gcgatcttcc gaggaaaacc atcggctacc cgctcgccgg cagcctgctg ccgtggatcg
    36841 acaaggacct cggcaacgga caaagccgcg aggagtggaa agcccaggcg gaggcgaaca
    36901 agattctcgg ccgtggcaat gggaaaaacg gagccaaggc cattcccatg gacggcgtct
    36961 gcgtgcgcgt cggggcgatg cggtgccata gccaggccat gaccatcaag ctgcgccggc
    37021 cggtgcccat ggacgaggtg gaggggatgc tcgccgacgc ccatgactgg gtgaaagtcg
    37081 tccccaatcg gcgggaggaa acgctggccg agctcacccc ggcggccgtg accggcaagc
    37141 tgacggtgcc ggtaggccgg ctgcgcaagc tccccatggg ggacgactac ctggcggcct
    37201 ttaccgtggg cgatcagctc ctttgggggg ccgccgagcc cctccggcgc atggtccgta
    37261 tccttttgga cgccggggtt aagcagtaat aaatcaagaa agtttctcga tttgcaggcc
    37321 taagaccatg atgttaaggt acttggtcct tctaagaaga ctcaggggtg ggcaagacag
    37381 tgggcagagc gatcaccgcc gcgatccttg caaccgcgct agcgctcgca gcgccgttcg
    37441 ccgcgcatgc agcgggcctg ggcaggctcc atgttctttc cccgctcgga caacccctta
    37501 acgccgaaat cgaaatcgtc gcgctgcggc ccggcgaaga agaaggcctg accgcgcgcg
    37561 tcgctgggca ggaggcgttt gctgccgcgg gcatagagcc gggcgcggtg ttgaactcca
    37621 tgcgtttcgc gatcgagcgt agcggagggc ggcgcatcct tcgcgtgacc acgctgcagc
    37681 cggtcaacga gccgttcgtc gagctgctcg tcgagctgca atgggcgacc ggccgcctgg
    37741 tgcgcgaata cactttcctg ctcgacccgc ccgagtacaa ggcgcgcgac gcgatggccg
    37801 ccgcgccgaa gcctgcgcct gccgaggtca agcccgcccc ggccgaggcc aagcctgcgc
    37861 ccgccgagcc gccgaagccg gtcccgccgc tggtcgaagc caagccgatc gagccgaccc
    37921 cggcgccgcc ggtcattgcg gccgctccgg tccccgccgc cgaggcggaa aagccggcgc
    37981 ctgtcgcgga gccggcgaag gaagaggccg ccaagcccga ggcggcaaag gaagagccaa
    38041 cccagccgga ggcggcgaag gaagaggcgg ccaaaccgga ggccgccaag gaagagctgg
    38101 tcaaggaacc cgaaggggcc gcgaagacgt acgaggtcgc gaagggcgac acgctgggca
    38161 agatcgcgca ggacaacctg ccgcccggca tcagcctcaa ccagatgctg atcgcgatct
    38221 atcgcgcgaa tcaggacgcc ttcattcgcg aaaacctgaa cctggtgcgc gccggacgca
    38281 tcctcaacat cccgtcggcc gattcgatcg gcagcgtgga tgcggacgaa gcgaaccgcc
    38341 tcgtgcgcgc gcacatggcc gagttccgcg aataccgcag ccggctcgcc gcggttccgg
    38401 cgacggcgga agccgcgccc ggcccgcgcg aaactgcggg cacgatcgag cccaagccgg
    38461 cagcgccagc gccggccgct ccgcaagatc aggtacggct ctcgaaggcc gatccgcaaa
    38521 agccgtctgc gtcgggcgcg gccgcggcgc gcggggacga tctggtggcg cgcgagcgcg
    38581 ccctgaagga ggcgcagtcg cgcatcggcg acctcgagaa gaacgtcgcc gacctgcaga
    38641 agctgctcga gctcaagaac cagcagctcg ccgagctgga gaggaaggcc ggcgcgaagc
    38701 ccgctcccgc ggcgcccgtg ccggctcccg caccggctgc aaaggctcct gaaaaacccg
    38761 caccggaagc gccgaagccg gtcgtcgaag cgcccaagcc cgcccccacg cctgcgccgg
    38821 ctgcgcctgc gcccgcggcg aagccgactc ccaagccggc gccaccgccc gcaccggaac
    38881 cgagcctcct cgacgagttc ctcgacaacc cggtggcgct cgccgggctg ggcggcgtgg
    38941 tgctgctgct gctcctttac gcggtctttg catggagaaa aaaaaagacc gcacaagcca
    39001 aattccagga tagcgtgatc ggcgccgccg cggctggcgc ggcggcggga gcgggcatga
    39061 ccgagccgac gctcggtgcg gcaacgacgg catcgaccca gcctgtagtc agcacccagc
    39121 ctgccgcggc ggccgaagag gtcgacccga tcgccgaggc cgacgtctac atggcctacg
    39181 gccgcgatgc gcaggccgag gaaatcctga aagaggcgct gcagaaggac gcgagccgga
    39241 tcccggtgca cgcgaagctc ctcgagatct acgccaagcg ccaggacacc aaagctttcg
    39301 agcagagcgc gctcaaactc aagaacctga ccaacggcgc cggcccggaa tgggacaagg
    39361 cggcggtgct cggcaagtcc ctcgatccga acaacggcct gtacgccggt gctacgtccg
    39421 ctgccgcagc attcccggcc gccgccgcag cgcctgccgc gcccgctccg acgctcgatt
    39481 tcgacatcgg cggcgcgacg cagggggtgt cggcctcggc gcccgatctc acgctcgagt
    39541 tggcaccgaa gtccgccgac gggggcctgg acttcgatgt cagcggatcg accgagaaaa
    39601 aggacgagga aaccgtggtg gcgccgcgcc cgcaggccaa ggacgcgggc ctcgatttcg
    39661 atctcgacct ggggtccgca gcccccgagt cgaagcccgc gtcggccggg cctgccccgg
    39721 ccgcggatcc cgggctgacc ttcgatctca acctcgatct cggtaccgag aagaaaaccg
    39781 agccggtctc cggggccggg cccgacctct ccaccatcag cctcgatctc gacaccgcgg
    39841 actcgggtgc cgccgcccct gccggcaccg acccgaagtg gcaggaaatc gcgaccaagc
    39901 tcgacctggc caaggcctac gaagaaatgg gcgacaagga cggcgcgcgc gagctgctga
    39961 acgaggtgat gaaagacggc gacggcgcgc agaaaggcca ggcgcagcag cttctcgcca
    40021 agctgggcta ggttttctcc atcggcagga tcgctctagg gctcgaatac gacggcagcc
    40081 gctttctcgg ctggcagacc cagcccggcg gcagcaccgt ccaggatgct cttgaggcgg
    40141 ccctcgcctc gattgccggc acacaagtgt ccaccaccgc cgcggggcgc accgatcgcg
    40201 gcgtccatgc gcgcatgcag gtggcgcact tcgataccgc tgtgaagcgc cccgagtcgg
    40261 cctgggtccg cggcgtaaac gccctcctcc ccgacgcggt ggccgtgctc tggtcgacgc
    40321 aggtcgcgga cgagttccat gcgcgctaca gcgcgctttc gcgcacctac cgatacgagc
    40381 tgctcaaccg accggtgcgc gcggcgcttt cggcgcacta cgccggctgg ttccacctgc
    40441 cgcttgacgt ggaaaagatg cgcgaggccg cggcgctgct cctcggcgag cacgactttt
    40501 cggctttccg ctcttccgaa tgccaggcaa agacgccggt acggaacatc catgcgttca
    40561 cgattgatca ggagggtgaa agaatagtct tcgtcgtgcg cgccaacgcg ttcctccacc
    40621 acatggtgcg caacctcgtg ggcacgctgg tgtacgtcgg caagggcaag cacccgccgc
    40681 agtgggtcaa ggaggtcctg cgctcggggg atcgcgccaa ggcggccccc acgttcggtc
    40741 cggaagggct ataccttgag agaatcgagt accagcccaa atggggactg ccaatctgag
    40801 aacccgggtg aaaatctgcg gcattacgcg cactgcggat gcagccgccg cggcgcgcgc
    40861 cggcgcggat gcgatcgggc tggtgttcta tccgccgagc ccgcgctttc tctcgatcga
    40921 gcgcgcgcga gaaatccgcg acgcgctgcc gccgttcgtg cagaccgtgg cgctcttcgt
    40981 caacgccgac gccgcccagg tctcccaggt gatcggccgc gtgcatccgg cgctgctgca
    41041 gttccacggc gacgaagcgc ccgatttctg cgcgcagttc gggctgccct tcgtcaaggc
    41101 ttgcagagtg aaaaaagggg tgtctgcgct agaatacctg cgcccttttt caagcgcggc
    41161 ggcgtggttg ttcgacagcc atgttccgga gtacggcggc gtcggcgaga gcttcgactg
    41221 gtctctggtt cccgtgaccg acaaacccgt catcctgtcc ggcggactct cccaggccaa
    41281 tgtggcggcg gcgatccggc gcgtcaaacc ctggggcgtg gacgtgtcga gcggcgtcga
    41341 gtcggcgaaa ggcatcaagg acgcggcgaa gattgccgcc ttcatggcag aggtacgcaa
    41401 tgcggatgta tgacctgccc gacgcgcgcg ggcatttcgg tccttacggt ggcgtgttcg
    41461 tcgccgagac gctcacggca gcgctcgacg agctgcgcct cgcctacgag cgcgcgcgca
    41521 aggatcccgc attcgaggcc gagttccgct acgagctcaa gcactacgtc gggcgcccga
    41581 gtccggttta ttttgcaaaa agactgactg agaaatgcgg cggcgcccgg atctacatca
    41641 agcgcgagga cctgaaccac accggcgcgc acaagatcaa caacaccatc gggcaggcgc
    41701 tgctcgcgcg caacatgaag aaaccgcgag tcatcgcgga gaccggcgcc ggcatgcacg
    41761 gcgtcgccac cgcgacggtg gccgcgcgct acggcatgga gtgcgtggtg tacatgggct
    41821 cggaggacat caagcgccag gccgccaacg ttcaccgcat gggcgtgctg ggcgcgaaag
    41881 tggtgccggt cgagtcgggc tccaagaccc tgaaggacgc gctcaacgag gcgatgcgcg
    41941 actgggtcac caacgtggag agcaccttct acatcatcgg caccgtcgcc ggcccccacc
    42001 cctatccgat gatggtgcgc gacttccagt ccgtcatcgg cgaagagagc atcgcgcaga
    42061 tgcaggagct cgcggggcgg cagcccgacg cggtgatcgc ctgcgtcggc ggcggctcca
    42121 atgccatggg gatcttttac ccgtacctca agcacgagaa gactcgcctg gtcggcgtgg
    42181 aagccgcggg tgaggggctg gaaacaggga ggcacgccgc gtcgctttgc gcgggcaagc
    42241 cgggggtgct gcacggcaac cgcacctacc tcctgcagga cgccaatggc cagatcaccg
    42301 acacgcactc ggtatcggcg gggctcgact atccgggcgt cggccccgag cacgcctggc
    42361 tcaaggacag cggtcgcgcc gagtacgtcg ccatcgacga cgaggaggcg ctcaaggcgt
    42421 tccacgagct gtgccgcgcc gagggcatca ttccggcgct cgaatcggcg cacgcggtgg
    42481 cgcaggcgat gaagatggcg ccggccatgg cgaaggacgc gattcttctg gtgaacctgt
    42541 ccggccgcgg cgacaaggac ctgcacaccg tcgccgagcg ctcggggaag aagctttgat
    42601 gtcgcgaatc cagggccgct tccaggcgct ggcgcgcgac aagcgcaaag cactcatccc
    42661 ctatatcacc gcgggcgacc cgcatccttc gctcaccgtg ccgctcatgc acgcgctggt
    42721 cgaggcggga gccgacatcc tggagctcgg cgtgccgttc tccgacccga tggccgacgg
    42781 cccggtggtc cagcgcgccg gcgagcgcgc gctaaagcac ggggttggcc tttccgatgt
    42841 cttgaggttg gtagctgaat tcagaaaaag cgataacgca accccgatcg tcctcatggg
    42901 ctacgccaac ccgatcgaag cgatgggcgt ggagaagttc gtcgccgcgg ccaaggccgc
    42961 cgacatcgac ggcgtgatcg tcgtcgacta cccgccggaa gagtgcgagc agttcgctgc
    43021 gcttgccaag aagcacggca tcgatccggt cttcctgctc gcaccgacct ccaccgagaa
    43081 gcgtatccag caggtcgccc gggtcggcag cggctacctc tactacgttt cgctgcgtgg
    43141 cgtcaccggg gcaggtcacc tcgatttttc ggaggtcagc tcgaaaattc cggcgatccg
    43201 cgccgcgacc aagctgccga tcggagtggg attcggcatc cgcgacgccg aatcggcgcg
    43261 ccgcgtggcg cagtccgccg atgcagtcgt cattggaagc cgtatcattc aggaaattga
    43321 agccggcacc gccgacggcg cggtcgcacg cgtcaaggcg ttcctgaaac ccatcagaca
    43381 ggcgctcgac ggatgagctg gttacagaaa ctcctgccac cgaagatcca gcgcaccggc
    43441 ccgggggcgc gcaagaccgt gccggagggc ctctggacca agtgcgcggc ctgcgacgcg
    43501 gtgctctaca gcactgacct cgagaagaac ctcaacgtgt gcccgaagtg cggccaccac
    43561 gagcgcctcg gcgcgcggcc gcggctcgac gcgctgctcg accccgacgg ccgcttcgag
    43621 atcggctcgg aggtgctgcc gctcgacccg ctcaagttca aggactcgcg ccgctacagc
    43681 gagcggctgg cggaagccca ggaggagacc gctgaaggcg acgcgctggt ggtgatgcag
    43741 ggctcgatca aggcgctcgg catcgtgtgc gcggcgttcg agttcgattt catgggcggc
    43801 tcgatgggct cggtggtcgg cgagcgcttc gtgcgcgggg tgcgagtggc gatcgagcag
    43861 aaaatgcctt tcgtctgcgt caccgcctcg ggcggagcgc gcatgcagga aggcgcgctg
    43921 tcgctcgtgc agatggcgaa gaccacggcg gcgctgaccg accttgccga gcgcgggctg
    43981 ccgttcgtct cggtgctctc cgacccgacg atgggcgggg tgtcggcgag cttcgccatg
    44041 attggcgacg tggtgatcgc cgagcccaag gcgctcatcg gcttcgccgg gccgcgcgtt
    44101 atcgagcaga ccgtgcgtca gaagctgccc gaaggcttcc agcgcgccga gttcctgctg
    44161 gagaagggcg cgatcgacat gatcgtcgac cgccgccagc tgcgcgacaa gctgcatgcc
    44221 gtgctttctc tcctgcaggg gctgcccgcc gaaacaccgg cgtgacgttg cttgcggagg
    44281 tcgtgaagac ctcgagcacc gtggcggcga cgtccagccg tctggagaag acccgcttga
    44341 tcgccgaatg cctgcgttcg ctctcgcccg acgagatcga gatcgcgctc ccttatctca
    44401 gcggcgacat gcgccagggc aagctcgccc tcggctattc gagcctcaag gccgccatgg
    44461 gccgcccggc gtcggtcccc agcctctccg tcatggacgt ggatcgcgcg ttccaggatc
    44521 tcaagaccgt caagggcaag ggggccgcgt ccactcgcga gcgccggttg aaggaactat
    44581 tcgggaaggc aacggcagag gagcaagact ttctgctgcg gctgatcgtc ggggagctgc
    44641 gccaaggtgc gctcgaaggc gtaatgctcg aggcggtcgc cgcggcggcc ggcttgcctg
    44701 cggccgaggt gcggcgggca gcaaccttcg ccggcggcat cgcgcccgtc gcctgtgcgg
    44761 cgctcgccgg cggcgccaag gctctggagc ggttctcgat ccgcctgatg cagccggtgc
    44821 tgccgatgct cgcccagccc gccgaggacc ttgccgccgc gctcgcgcag ctcggcgaag
    44881 cgctcctcga atggaagctg gacggcgcgc gcatccaggt gcacaagtcc ggcggtgaaa
    44941 tccgcgtgtt cacgcgcaac ctgaacgacg tcaccgcgcg ggtgccggaa gtggtttccg
    45001 agctgagaat gattgatgct gatctgattc tcgacggtga agcgattgcg ctgcgtgccg
    45061 acggccgacc gcacccgttt cagatcacca tgcggcgctt cggccgcaag ctcgaggtcg
    45121 aggcgctgcg cgacgagctg ccgctttcgg tgttcttttt cgactgcctg ctgcgcgacg
    45181 gcgaggccct cgttgaccgt ggtgccgccg cgcgccgccg ggtcctggag gagtcgctgc
    45241 cgaaggcgct cctggcgcct tcccttacca ccggtgacct gggaatggcc gaggcgttcc
    45301 aggccgatgc cttggcgcgg ggccacgaag gtttgatggc gaaggcgccc gccgcgccgt
    45361 acgaatccgg tcgccgcggc gccggctggc tgaagatcaa gagcgcgcac acgctcgacc
    45421 tcgtggtgct ggccgcggag tggggcagcg gccggcgccg cggccggctt tccaacctgc
    45481 acctcggggc gcgcgatccg aagacgggcg gcttcgtgat gctcggcaag accttcaagg
    45541 gcctcaccga tgagttgctc gagtggcaga cgcgcgagct tcaagcacgc gaaacgggcc
    45601 gggacgaatg gacggtgcac gtgcgcccgg agctcgtggt cgagatcgct ttcaacgacg
    45661 tccagggaag cccgcactat cccggcggaa tggcgctgcg cttcgcgcgc gtcaaaggct
    45721 accgcccgga caagcgcccg gaggaagccg atacgatcga caccgtgcgc gcgatctacc
    45781 agcggggtct tgcgtgaaaa gcctggacga gtggctcgcc ttcatcgagc gccagcatcc
    45841 ccagccgatt gcgcttggcc tggacagggt gagggaagtc ctcgcgcgaa tgaacatccg
    45901 cctgcagtgc ccggtgatca cggtcggcgg aaccaacggg aagggctcga cctgcgccat
    45961 gctcgagtcg attctgcgcg ccggccgcta ccgcaccggc ctctatacct cgccgcacct
    46021 cgtcaactac aacgagcgag tgcgcatcgc gggtgttgag ctggaagaca ctgcgctgtg
    46081 cgaggcgttc gcggcggtcg agcgagcgcg gggcggcgtc ccgctgacct acttcgaatt
    46141 cggcacgctg gcagcgtttt gggtttttgc tagagagaag attgagactg cagtgctaga
    46201 ggtcggcctt ggtggcagac tcgacgccgt caacgtgctc gatcccgact gcgcggtatt
    46261 gacgagcgtc ggtatcgacc acgtggacta cctgggcccg gaccgcgagt cgatcggccg
    46321 cgagaaggcg ggcatcttcc gcgccggccg gccggcggtg atcgccgagc cggatccgcc
    46381 gcagtcggtg atgcgcgccg cggcagagaa gctgctgctc ggaaaggaat tcggctttac
    46441 ccggcaggaa aaccagtgga cctattgggg tccgcgcggc cagcgggccg ggctcgccca
    46501 cccggcgctg cggggaagca ttcagctgcg gaacgccgcc gcggcgctgt gcgcgctcga
    46561 cgcgatcgag ctcgccatcg ccatgcagga cgtgcgacgc gggcttgccg aggtgacgct
    46621 gccggcgcgt ttccaggtac tgcctggccg gccgcaggtg atcctcgacg ttgcgcacaa
    46681 cgtggaggcc gcgcagaccc tcgccgacaa tctgctgagc tcaggctatt caagcgaaag
    46741 cattgccgtg tgcgggatgc ttcgcgacaa ggacatcgcc ggggtgctgc gcgcgctcgc
    46801 gccgcgcatc acgcgctggc atttcgcttc gctgccgggt gcgcgcggcg cgtccgcgga
    46861 ggagctggcg agaaatattg atggggcggt ggaaaagttc gaatctccga cgcaagcgtt
    46921 cgtcgcggca aagggacgcg cggaggaggg tgataagatc gtcgtgttcg gttccttcct
    46981 cacagtcggc gaagtgatgg catggctgca gaaaaacaag acgtcgaaac gctgaagcgc
    47041 aggggacgca ggcggctcgt cggcgcggtc gcgctggtgc tcgccgcggt gatcgttctg
    47101 ccgatggtgt tcgaccccga gccgcgcgga acgccctctg cggtcaacgt gcgcattccc
    47161 ggcgaagatg acacgccctt cgccccgaaa ttgccgccca ggaagaaggc cgacgcggct
    47221 ccaaaagagg aattcgtcgt ccaggtcggc gcctttgcca gcccgcaggg cgcgatcgcc
    47281 aagctcgagt cggcgaagat gccctacacc acagaggcga tgcagggcaa tctgacgcgc
    47341 gtgcgcgccg ggccgttcgc cacgcgcgaa gcggccgaga aggcgctgga gcagctcaaa
    47401 gggctcggct tcgagcccgg agccgtggcg ccgaaagccg gatgacctgg ctcgactacg
    47461 gcgtcatcgt ggtgctgctg ctctcgatcg cctggggagc gtggcgcggg ctggtgcacg
    47521 aggtgctctc cctcgccgga tggatcatgg ccttcctcgc cgccaatctg ctcgccgcgc
    47581 cgctctccga gactttcccg gccaacatgc ggccggagtt ccgcgtggtg ggggcgttct
    47641 tgctggtctt cgtcgggacg ctcgtcctcg tgaccctgct gacggcgctg gtcaccaagt
    47701 tcatcagggt ctccgtcctg caaagcctgg accgctggct cggcgcgctg ttcgggctga
    47761 tgcgcgggct ggtgctggtc gttgcttttg ccgtgatcgc gggcctcacc tcgtttccgg
    47821 ccacgcccat ctggaaggac tcggcaaccg gctattcgct cgcccagacg gtgatccagc
    47881 tcaagccctg gctgccgccc gcgctcgcaa gtcgcttgaa atataattaa cccttaacca
    47941 cacccgggaa cgcgtctccc atgtgcggga tcctcggaat cgtcgcccgt tcgcccgtca
    48001 accagttgct ctacgacggg ctcctgctgc tccagcatcg cggccaggac gccgccggca
    48061 tcgtcacgtc cgagcgcaac tccttccata tgtacaaggc gccgggaacg gtgcgcgacg
    48121 tgttccgcac ccgcaacatg cgctcgctgc cgggcaacgt cggcatcgca cattgccgct
    48181 acccgaccgc aggctcggcg ttcaacgcgg ccgaggcgca gccgttctac gtcaactcgc
    48241 ccttcggcat cgtgctcggc cacaacggca acctgacgaa ttccgatcag ctgaaggagg
    48301 agatgttccg cgccgaccgc cggcacatca acaccgggtc ggactccgag gtgctggtca
    48361 acgtgctcgc gcacgagctg gaacaggcct cggtgaagct gcggctcgat ccgcagacga
    48421 tcttcaccgc ggtggccaac gtccatcgcc gcgtgcgcgg cgcctacgcc gtggtggcga
    48481 tgatcgccgg ctacggcgtg ctcgcgttcc gcgatccgtt cggcatccgc ccggcggtga
    48541 tcggctacaa cgagaccaac gccggcaccg aatacatggt cgcctccgag tcggtagcgg
    48601 tggacgcgct cggcttccgg gtgctgcgcg acatcgggcc cggcgaggcg gtgttcttcg
    48661 acgaggacgg caggttccac caccagcagt gcgcgccgca cgcgagcctg aacccgtgca
    48721 tcttcgagta cgtgtacctc gcgcgccccg actcgttgat cgacggcgct tcggtatacg
    48781 agtcgcggct caacatgggc gaaaagctcg cggagaagat ccggcgccag taccgccacc
    48841 ttcaggtcga cgtggtcatc ccgatcccgg actcgagccg gccttcggcg ctgcagctcg
    48901 ccgtgggcct gggcgtgcct taccgcgagg gcttcgtcaa gaaccgctac atcggccgca
    48961 ccttcatcat gcccggccag gcggtgcgca cgagctcggt gcgccagaag ctgaacccga
    49021 tggcgatgga gttcagcggc aagaacgtgc tgctggtcga cgattccatc gtgcgcggca
    49081 ccaccagccg cgagatcgtg cagatggcgc gcgagtccgg cgcgcgcaag gtgttcttcg
    49141 cctcggccgc gccgccggtg cgcttcccga acgtctacgg catcgacatg ccgacgcgcg
    49201 ccgagctgat cgccgcgcac cgcagcgaag acgaggtggc gcgcgagatc ggcgccgacg
    49261 cgctggtcta ccaggacctc gatgccctca aggacgcggt gcgacgctgc aacccgaagc
    49321 tcacgagctt cgagacctcg tgcttcgacg ggatctacat caccggcgac atcaccaccg
    49381 actacctgcg ctccatcgag atccggcgcc acgccaagcg cgattccggc gacgacgaag
    49441 cgccgcagct ggacctgaat ttggctgcct gatatataat tcttctttcg cgccgatttg
    49501 agccacagct tgcgggcgcg ttacaaattc cgctaaagcg tgccgagaga acccggtccg
    49561 ctaggaaaga gaggccgggt ttttttatgg caaaagacta cgagctggca accaagggcg
    49621 tacgcgcggg cacggcgcgc accgagttcc aggagcactc cgaggcgctg ttcctcacct
    49681 cgagcttcgt attcaagaat gcgaagcagg ccgcggcgcg cttcgccggc gaagacgccg
    49741 ggatggtgta cacgcgctac accaacccca cggtctcgat gttccaggac cggctggcgg
    49801 cgctcgaagg cgccgagagc tgcgtgggca ctgcctcggg catggcggcg atcctcgcca
    49861 cggcgatggt gcacctcagg agcggcgacc acgtggtgtg ctccaacgcg gtgttcggcg
    49921 ccaccatcca gctcttcaac acgattttct cgcgcttcgg catcgagacc agctatgttt
    49981 ctcccacccg gaccgacgag tgggcgcgcg ccgtcaagaa aaacaccaga ctcttctttc
    50041 tcgaaacgcc gtccaatccg ctcaccgaga tctcggacat tcctgccctg gccaaggtgg
    50101 cgaaaggagc gggcgcgctg ctcgccgtcg acaacgtgtt ctgcaccccc gcgctgcagc
    50161 ggccgctgga gctcggcgcc gatctggtga ttcactcggc gaccaagtac ctcgacgggc
    50221 agggcagggt gatcgcgggt gccgtcgccg gctccaaagc cctggtcggc gatccgatga
    50281 ctaccttcct gcgcaccgcg gggccgacgc tctcgccgtt caacgcgtgg gtggtgctga
    50341 aagggctgga gaccctcgag ctcaggatgc tggcgcagtc ggctgcggcg ctggagcttg
    50401 cgcgctggct ggaaagccac cccaaagtgt cgcgcgtcca ttacccggga cttgagtccc
    50461 acccccagca ccagctggcg aagcgccagc agcgcggcgg cggcgcggtg gtctcgttcg
    50521 aggtggaggg cggccgcgcc gcggcttggc gcgtgatcga ctcgacgcgg ctcatgtccc
    50581 tgaccgccaa cctgggcgac gtcaagacca ccatcatcca tcccgcgagc accacccatg
    50641 gcaggatatc gccggaagcg cgcgcggccg ccggcattac cgatggcctg atccgcgtcg
    50701 cggtcggcct ggaggcggta tcggacctga aagaggacct gcagcgagga ctggaagcgg
    50761 aacagcccag tttctcctag tcgcggccgc ctacttcggc gcggcgaagc tctcgctgct
    50821 ggcggcgatt ccgccgggat acgcgaccgc catctggccg ccgtcgggca tcgccgtggc
    50881 cgccctgctg ctgggcgggg gccgtctgtg gccggcggtc tgggcgggaa gcttcgccgc
    50941 caatatcacc atcgattggg cctacgccgc ctcgaccatc atcgctacgg gcagcacgct
    51001 gcaaaccctg gcgatcgcgg gccttgtgcg cgagcacatc ggggtgctgc accgcttcga
    51061 gaccgtcgcg cacgtcgtcc gattcgtgct gatcgccgcg ctcggctgcg ccatcgcgcc
    51121 gaccttcgcg ttgctgccgc tcggcgcgtt ctacgagatg tcggcggtcg agctggcggg
    51181 caactgggcg acctggtggc agggcgatct ctgcggcgtg ctggccttcg cgccgctgat
    51241 cctgggctgg ctcgcgccgc gcgccacgac ctggacgccc ggcaagctgc tggaggcggg
    51301 cgtgttcggc gtcgtcctgc tcggcgccag ccagctcgtg ctgagcgccg gcttcgagcg
    51361 cgccttcctc atcgtgccgt tcatcgtctg ggcggcgttc cgcttcgagc agcgggaggt
    51421 gaccaccgcg accgcggcgg tgtgcgcgat cgcgctctgg tccatgccgc aggagtcgtt
    51481 cgtgctgctg ccgctcttcg tcagcacgct ggtgttcacc gggctggtgt tgagcgtggc
    51541 tctgaaacag ctcgagcgcg ccaggacgga gctggaatcg cgcgtgcggg agcgcaccgc
    51601 cgagctcgag gaagccaagc aggccgcgga gaaggcgaac gaagcgaagt cgcagttcct
    51661 cgccaacatg tcgcacgagc tgcgcacgcc gctcaacagc ctgctgatcc tcgcgcgcct
    51721 cctcgccgac aaccccggcg gcaagctcga tgccaagcag gtgaaattcg cccagaccat
    51781 ccacgcctcc ggcctcgacc tgctttcgct gatcaacgac ctgctcgatc tggcgaagat
    51841 cgaatccggc gccgtcaccg cgttgaacgt cgcgccggcc cggttcgccg acctgcgcga
    51901 ggagctcgag cgcactttcc agcaggtggc gcacgacaag agcctcgcct tctcgatcca
    51961 catcgatccg gcgctgccgc cgacgctgcg caccgacgcc gggcggctga agcaggtgct
    52021 gaagaacctg ctcgccaacg ctttcaagtt caccagccgc ggcagcgtgg ccctgcgcat
    52081 cgcaccgggc gcgccgggct acgtcgcgtt cgcggtgagc gacacgggaa tcggcatcgc
    52141 cgcggacaag cagcagctca tcttcgaagc tttccgtcag gccgacggta cgaccagccg
    52201 cctgtacggc ggcaccgggc tcggcttatc gatcagccgc gagctgacgc ggctcctcgg
    52261 cggcgagctc gggctcgcga gcgtgcccgg cgaaggcagc acctttacgc tggttctgcc
    52321 gctttcggat cagcgcacga cgcttgctgc ctgagaaggc aaacccggcg gccagcgccg
    52381 cgtagaccgc gaacatcgcg cgcggcgaca ggcaggtctc gaatccgggc ctgaagcgct
    52441 tggaaacgac gtagtagtcc accacgtagg cgatcgccga gagcaccgag gcggtcgcaa
    52501 gtcgttgcgg acgcggttgc gcagcgagcg ccgcttcgag aaatgccgcc caccagaccg
    52561 aggcggccgt atggatgccc agaccgagga gcgtattgcg gccgtaccgg ccgtcgtgct
    52621 gcggcggctc acctccgtca tagatatgga cgatggcatt catcggcctc gcggcgtggc
    52681 cgttctccac ccggctcgcg accgcggcgg cagcagcgga ggcgagggca cccaggattc
    52741 cggtcgtgag gatcttcttg ggaagcagca tgggttggct tccctgccca gcaaactacg
    52801 tgcctaaaaa cgcacgaggc gcggcccgcg ccggctgact tcgacgtagc cgccgcggcc
    52861 gtaccagtcg ggcaggaccc agcgctcgca gcgctggccg tcgacctcga gcgcgtgtcg
    52921 gcccgggcgg tgggtatggc cgtggatcag gcggcgcacg ccgtgacggc gcagcgcctg
    52981 gcgcaccgcc tcgtcgttga cgtccatgat ctctgccgtt ttttcctgca ccacctgctt
    53041 gctcttctcg cgcatctgtc cgaccgcgcg ccggcgctcc ggaagcggtt tcgccaggaa
    53101 gtccgcctgg aaggcgggat cgcgcgcctt gcgccgccag gcctggtagt caaggtcgtc
    53161 ggtgcacagc gtgtcgccgt gcaccagcag ggttttttcc ccctggatgg tctccacgac
    53221 ggggtcctgg aggagctttg ccccggtcgc ggcgcagaat cgctcgccga tgaggaaatc
    53281 tcggttgccg tgcatcaggc tgaccggcac cccgcgctgc gtcaggtcgg cgagcaggcc
    53341 ggcgaccacg gcgttgaaag gctcgccgag gtcatcatcg ccgatccagt actcgaagaa
    53401 gtcgcccagg atgtagaggg cctcggcggt gcgcgccttg tcttccagga aggcgatgaa
    53461 gcgctcagtc gcctccggcc gctcctcggt gaggtgcagg tcggagatga acagcgtggg
    53521 cactacggct cgggacctac ttcttcggct cgggcaccac ggtcatcgat tcgatgacga
    53581 tcgcctggcg cggaacgtcg ctgggaccct tgggcgtctt ggcgatcttc gtcacgacgt
    53641 ccatcccgga cagcaccttg ccgaaggccg cgtagccgtt gccgtcgccg cgcgggtcgc
    53701 cccagttgag gaacgagttg tcggcgacgt tgatgaagaa ctgggcggtg gcggaattcg
    53761 gatccgcggt gcgcgccatc gcgatcgtgc cgacctcgtt cttcagcccg gccttgattc
    53821 cgccctgcgc ctcgttgacg atcggcttgc gggtcggctt ctgctgcatg ctcgccgtca
    53881 tgccgccacc ctggatcatg aagccgtcga tgacccggtg gaacaccgta ccgttgtaat
    53941 ggccgtcgcg cacgtactgc aggaagttct ccaccgtctt cggcgccttg gccgggtaga
    54001 gctcgaggcg aatcgtcccg gcgctcgtct tcacgtccac ttgcgggtcg gcggccagcg
    54061 cgcctggagc gaccgccgcg gccgaaagga tgaagaggtg tttgaggttc atcctgctcc
    54121 ttcgtagatg atcttccact gcgcgccttc cttgacccag tactggcgct tgcgcatgac
    54181 gttggacagg ccgtcggaac ggtagtcctg gttgaacgtc accacgacga agtcgcgctc
    54241 gcgagggtag cggaacatcg ccacgcgcga cacgccgacc ttgatccagc gcttcgcgcc
    54301 gttcaccttg cgcttgtgct cggcccatgc ggcaaggtcc tgtcccggcg ccgcgaagcg
    54361 gctcgagtag tgggcaaggt agcgctcggt gtcgcggctt tgccagtcgg cgcgccattg
    54421 ctcgaacgcg ccggccagcg ccgcgcgctc ggcggcgatg gtggcagcgt cgctccattc
    54481 gatttcgtcg gcgatgatca ccggagtgag gcccacctgc acgtggcgcc ccaccgactc
    54541 gaggtccggg ttggcgagca cgatgcagcc gtcgctcgcg cgcggcgggc ggctgtaaac
    54601 gtcggaggga acgccgtgca gccagatgcc gtagccgccg cgtccctgac ggcggtccca
    54661 ctcgttcggg tagttgatcg ggaacgcgcc tgcgccatag aagtcagtga gcttcttgcg
    54721 cggcaggttc gccgtgacgt gatagacgcc gacaggcgtc ttctggtcgc cttcgcgggt
    54781 tttctcgatg ccgtttttcc ccagagtgac gtagtagtcg gcgaccagcc gcggcgtgcc
    54841 cttgtcattg ccgaagacgt agagccgcga gcgtcgcgaa tccaccatca ggacgtgctt
    54901 ctgctcggcg tgaagctgca gcacgtagcg cggcaagcgg tcgtcgcccg ggcggtggcg
    54961 ctgcgcgcgc agccgcgcga gcgcctcttc gcgcagccca tcgatttttt cgcccggcac
    55021 cgtcttcgcg acgttgccga aggtctgcag cgggcgcgcg cgcgcgagca gcaggtcgcc
    55081 gcgcacgagg tgcgccaggc ggaagttcgg gtattcgcgg atcagcgcat ccacgcgctg
    55141 cagcgcttcg tccagccggt tggcttcgat cgactggaat acggtcgata gccggtcctc
    55201 cggggttgcg ctcgccgcaa ccgcggccca gcacagcaga accgacaaga ccaccgcgcg
    55261 catcagccgc ttttctcgtc caggattttc cagccgccgt ccgcccggat gagagtgagc
    55321 gtcttgcggc tattggactt gagcacgtcc gacttgtaat tctgctggaa cgtcaccgtc
    55381 gcgcgatcgg cgccgttcat ggtcactttg ggcgatgcca ggctcaccga gatcgacttc
    55441 ggcgcgctga tccgggcgcg ccgggtcttt tcccagctgt cgcgcgcttc gccgccgggc
    55501 gtcttgaagt ccttggcgta gtaggcgagg taggcatcgg cgtccttctt ggaccacgcc
    55561 tccgcccaac cgtttacggc ttgcaggatc tcggcggaga gatcggcggc cggcttctcg
    55621 ggagccggct tggcgaccgc taccaccggg ggcttcgcgg cctccttcgg agcctctttg
    55681 ggggtcggtt gcggcttggc cgcctctttg ggcgcgaccg ccaccgcggt cgcgctcggg
    55741 cgggccacca gctcgcgcac cagcgagagc ttgttcttcg ccgcggtgtt cgacgaatcg
    55801 atctgcaggg ccttgtcgta ggcctggctc gcgagcttgg cgtacacgtc gcccaggttc
    55861 tcgtaggccg tggcgtagct cggatgggtg cggatcgact gctcgagcgc cgcgcgcgcc
    55921 ctgtcgtact gaccttgcga ggcgtagatg acggcgaggt tgttgtacgg ctccggcagc
    55981 tccgggtaat cctgggtgag cttcgtaaag atctcgatcg cgtccttgct cttgccttgc
    56041 tcggtgaaga tgagcccctt caggaagcgc gcctgcgcat cgcgcggctt cgccgcgagc
    56101 accttgttga cgagatcgag cgcctgcttg tgctgcccgg ctttcagcag cttggcggcg
    56161 tcctccacgt cgtcggcgca aaccggcatc gcagcggcaa gcgcgaccaa gacccaaccc
    56221 cagcgggcgg cgtaactcag cattgtgcta atattttcaa ggccctacga aagcatcggc
    56281 attctagact aatcctgaag atcttcaact ccctcggcag ggcaaagcag gaatttattc
    56341 ccctgcaccc cggcgaagtc cggatgtacg tctgcggcat cacggtttac gactactgcc
    56401 acctcggcca cgcccgcttt ctggtcgcct tcgacatggt gcagcgctgg ctgcgcgcga
    56461 gcggctatcg cgtgacttat gtgcgcaata tcacggacat cgaggacaag atcatccgcc
    56521 gcgcgcagga aaacggcgag tccatcgatg ccctgacctc gcgcttcatc cgcgccatgg
    56581 acgaggacgc cgccgctctt ggcgtgcaga agcccgacca cgagccgcgc gcaacgcagt
    56641 acgtgagcga catgctgagg atcattgagg ctttgcagga gaggaagctc gcctatcaag
    56701 ccgccagcgg agacgtgaac tacgcggtga ggcgcttccc cggctacggc aagctctcgg
    56761 gcaagtcgct cgacgaattg cgcgccggcg agcgcgtcga ggtcgacacc tccaagcagg
    56821 acccgctcga tttcgtcctg tggaagcgct ccaagccgga cgagcccaag tggccctcgc
    56881 cttggggcga ggaccggccg ggctggcaca tcgagtgctc cgccatgagc tcgaggctcc
    56941 tcggcgagca tttcgacatc cacggcggcg gcgaggacct gcagttcccg caccacgaga
    57001 acgagatcgc gcagtccgag ggcgcgagcg gccggaagtt cgtcaactac tggatgcaca
    57061 acgcgttcgt gcgggtcgac gaggagaaga tgtccaagtc gctcggcaac ttcttcaccg
    57121 tgcgcgacgt cctgaagaaa tacgacgccg aagtggtgcg cttttttatc ttgcgcgcgc
    57181 actaccgcac cgcactcaac tactcggacg cccacctcga cgatgcgaaa gccgccttga
    57241 ctcggctgta taccgccttg aaagactcaa gcacccggag tggaatcgac tggagcgaag
    57301 cgcacgccag gcgcttcaag gaagcgatgg acgacgattt cggcacgccc gaggccgttg
    57361 ccgtcctctt cgagctcgcc agccgggtca acagcggcga aaagcgcctt gccggccagc
    57421 tcaaggggct cggcggcgtg ctggggttgc tgcaacgcga tcccggcgcc tttcttcagg
    57481 cgaagcctgc gggcgtctcc gaccaatgga tcgcggaaaa aatcgtcgag cgcgaggcgg
    57541 ctcgcgggcg caaggactac gacgccgctg atcgcatccg gaaggaactg cttgagcaag
    57601 gcatcgtgct cgaggacaag ggcgggaaga cgagctggcg ccgcgcctag cgtgacttag
    57661 gtcacagact caggtgtctt gcctaagcag tttccccgat acgcgctcag gttatggagc
    57721 agtggggtgc gcgtcccgtc gcagctaccc ccagtggcgc gcgctgccaa aaataactcc
    57781 caatagttcc tagctgttac gaacattatc aattagttat gccctgccgg gacacaatgg
    57841 gcacaatccg gggggaagaa cgatagccgc cgggagagcg ggagatagat aggcaccatg
    57901 tctgagggga aagaatccgt cgccgcgaag ccgggcgggc agcgtccacc gctgccgccg
    57961 atccgcgaag atttgcgcct gtatcccggg ccggctcatg ccgacggctc gccgagctgg
    58021 cgcatcctcg atccggttcg caattcgttc ttcgagatcg gctggctcga gttcgagctg
    58081 ctcgcgcgct ggcgcggaca gcgcgacggc gctgcgttgc tcgagcaggt cgcggcggaa
    58141 acgccgctca agccctcgcc cgaggagctg aaggagctga tcgactttct ctcgacgaac
    58201 cagctgctct cgccgcgcag cgccatcgca caggaggtgc tgaaccgccg gctgcatgaa
    58261 tcgaagcatg cgtggtactt gcaggtgctg catcactatc ttttcttccg gctgccgctg
    58321 ttccggcccg acgcgtttct cgcccgcacc gtcggcgtga ccgacgtgtt gttcacgcgc
    58381 ggattcatgg tgttcgtgct cgtcctgctc gggctcgacc tctacctggt ctcgcgcgag
    58441 tggtacagcg tcgccgacgc gatggcgcgc atgttcacgc cgcaggcgtt cctgtactac
    58501 gccgtggcgg tctcgttttc caaggtggtc cacgaattcg cccacgccta cgcggcgcgg
    58561 cgctacggcg ttcgcgtgcc gaccatgggg gtcgcgttcc tggtgctgtg gccgttcctg
    58621 tacaccgaca cgagcgaaac ctggaagctc gccgaccggc gcaagcagct cgtcatcgcc
    58681 tcggcgggca tggtctcgga gctcacgctg gcggtgttct cgaccctgct ctgggcgctc
    58741 gctcccgaag gcggcgccaa gaacgttctg ttcgtcctgg cgagcacgac gtgggtggtg
    58801 accctcgcca tcaacctgag tcctttcatg cgcttcgacg gctatttcgt gctgtcggac
    58861 ctgctgggat tccccaacct gcacgagcgc agcttcgcgt gcgcgcgccg ctggatgcgc
    58921 gccacgttct tcggcctcga cgagccgctg accgagccgg gcctgcgccc gcggcagcgc
    58981 gccgggctga tcgtcttcgc gtggctgacg tggctgtacc ggctgaccgt gttcctcggc
    59041 gtcgcgctgc tcgtctacca catcgccttc aagctgctcg gcattttcct catgctggtc
    59101 gagctgatct ggttcatcgc gcggccggtg tggaaggaga tcggctacgt ctggaaggcg
    59161 cgaagcacgg tcaagatggc ctggcggccg gcgatcgtgc tgctcgcgct gatcaccgcg
    59221 ctggtctggc tgataccggt gtcctacgaa gttacggcgc cggcgatcct gcgggcgcag
    59281 gaagagcacg cggtgtatgc gccgttccct gcgcgggtga ccgcggtgcg ggtgtccgac
    59341 cgccagcgcg tgggcgccga tgtcgagctg gtcagcctcg aggcggtcga tctcgacgtg
    59401 cgcgagaaga aggccgacat ttcgatcgtg gcggcgcgcg ccgagctcgc gcgcatgccc
    59461 gccagcgtgc gcctgcagga gaactacggc gtgcttcagg agcgcctcgc ccaggcgctg
    59521 gcggagaagc aggccgtgcg cgacgagtac gggcggcagc agctgcgtgc ggcccaggcc
    59581 ggcatcgtcc gcgacttggc gcccgatctg ctcgccggcc gctggatcaa cccgcgccag
    59641 ctgctgatgc gcgtcgtctc ggaagacgtg ccgctcatcg aagcgtacgt cagcgaaagg
    59701 caggtcgcgg ccatcgttcc cggccagtcg gtgcgcttct tcccgcagct gcccgaccgc
    59761 ccggtcctga ccggcgaagt cgtcgccgtg gacaagtcgc cgcagaaaca attgtcgcgg
    59821 ccgctgctcg cctcgctgca cggcggcgcc gtcgtggtga agcaggacca gcacggctcg
    59881 ctggtcgcgc aggacgcgct gtttcgcgtg atcgtcaaac cggtgggcgc gcctcccaag
    59941 gtcgacgccg tgatccacgg cagcgtgcgc atcgagaccg ggttgcgctt cctggtggaa
    60001 aacttcgttt accgcattct ttcggtgctg atccgtgaaa gcggcatcta agaaacgaaa
    60061 gcccatgtcc aagaaatccg accgcgaatc gaaaaaggcc gtgcggcgca agaccagtgc
    60121 cgccccaacg aaaagacgcc ccccgcgtcg cacgcgcatg ctggcgctcg agccgcgcat
    60181 gctgttcgac ggcgcgctcg gaatcgatct cggcgccaaa gcggccgccg ccctgcaggg
    60241 agacgcagcg gcgcccgcgg aaagcaccac gccggcggcg ccgcaggcgc aggccggcgc
    60301 gccggccgcg cccgccgcgc ccgagaagag cgctgtcgaa aaaaccgccg agcggctcgg
    60361 catcgcgccc gaagcgccgg tgcgcaatga gatcgtgttc gtcgactcga ccatccaggg
    60421 ccacgaggcg ctgatcgccg ggcttagccc gaccgccaag gtcgtgatgc tcgacccgac
    60481 ccgcgatgcc ctcgaccaga tcgccgacta tatggacggc gagcgcaacg tcgacgcgat
    60541 ccacgtcctc agccacggca gcgccggcaa cctgatcctc gccgggcaaa cctactcggc
    60601 cgaccggttg acgcaggagt acgccaccga cctcgcgcgc atcggccacg cgctgagcgc
    60661 ggacggcgac atcctgctgt acggctgcga tgtcggcaaa ggcagcgccg gcgagcgctt
    60721 catcgacacg ctgagcgcaa tcaccggcgc ggacatcgcg gcctcggtcg acaagaccgg
    60781 cgcggccgac ctcggtggcg actggatgct cgaggtgaag acggggcagg tcgagaccgt
    60841 ctccatttcg ctcacgcacg agtgggattt cctgctgacc gcgccgccgg tcaacgtggg
    60901 cggcgcgacc ctcgacttcg acggcgccaa ccgcacgctc gtttccggcg tcgccggcgc
    60961 ggtgggcgcg gtctacaagt acgccgacgt cgcgaccatc aatggcgtgc agatcgatgc
    61021 ctacgtcacg atcagcggca tcaccaacgc gacgctcgtc aacatcgaca acgatgcacc
    61081 cgcgagctat gtcccgcccg cgggcacgaa tgcagccaac gtcttcgccc ccgagatcag
    61141 cgtcaccgcg gccaacggca agatcgactt cgcgatcaac ttcaaggatc cgaacggcaa
    61201 caacctgacg ctgctcaact tctacaacaa ctcgctcgac atcgacggcg cgggcggctt
    61261 ccaggaattc gtcgagtacg gcggcttcaa gtcggttacg acgggcaacc cgaccgacct
    61321 cgtcatcagc tcgtcccccg gcaacgaccg tctgcgcttt accggcagca gcgtctacaa
    61381 cggtctcatc gtgaacgacg tggggcgcgt gcaggcgaat ttcgacgcga tctcgacgct
    61441 gcagatctcg atgggcgcta ccggcaacac cggtggcctg cgccagtacg gctcgctgtt
    61501 ctccgcggtt tcatttggca gcccggtgac gattaccgcg ccgacttccg acctgctgac
    61561 caccgccgac accacgccga tcctgacggg cagcgttggc gctgctccgc tcggcggcgg
    61621 cgagaccttc agcgtgacgg tcaacggcac gacgtatacg accgccaacg gcctcggcat
    61681 cagcgggtcg acctggtcgc tggaattgcc cacggcgctc gcttccggcc agtacgacgt
    61741 gcgcgcggta cgcagctcgg ccgggctcga tatcgcggac cagacccagc tcgagctcac
    61801 catcaacgac gcgccgacgc tggccgacac ggcgcttacg ctcacggtgg ccgaggacgc
    61861 gccggcgccg gcgggagccg tcggctcgct gctctcgtca ttcactggtg gtatcagcga
    61921 cgtcaacgcg ggtgcggcga agggcattgc catcaccggt accaacgaga ccaacggcac
    61981 ctggtactac acgacgaacg gcggcaccac ttggaccgcg gtgaacgcga tcgacggcac
    62041 cgtgagcggg accaatgcgc tgctgctggc cgacaacgcg aacacacggc tctacttccg
    62101 gcccaacgcc gactacaacg gcagcgcacc ggcagcgctg acgctgcgcg cttgggacac
    62161 gacgagcggc acggcaggca ccaaggtcga cccgggttcg ggcggtggtt cgctcgcttt
    62221 ctccacggct tcggatgtga tcgacgtgac ggtcacgccg gcggccgatg cgccggtgaa
    62281 taccgtgccc ggggcgcagg gcaccgggct caatattccg ctgattttca gcacggcgaa
    62341 cggcaatgtg ctgagtgccg ccgacgtcga cggcaacctc gccaccgtgc gggtcacggt
    62401 gaccaacggc gtggccaacg tcaccctgtt cggcgctgcg acgatcagcg cgggcgccaa
    62461 cgggacgaac acgctgacga tttcgggcac gcaggcggac atcaacgcca cgctgaacgg
    62521 cctgcgctac acgccgaccg caggctttac cggcgcggcg acgttgacgc tgctgtcgac
    62581 cgacagcgcc gcgcagaccg attcggatgt cgtcaacatc aacgtgtcgg ccgtggcggc
    62641 cctgccacgc atcgatctga acgacggctc ggcgtccgac accatcgcgg acaactttgc
    62701 ctccggcggc tacgccggct cgagcggcag cgtcaatctc tggaccggca gctgggtcga
    62761 gagcgacccc gaaggcgcgg cgcagagcgc cgccgcgggc gacgtccggg tgactgccgg
    62821 cgcgctagta ctcggtgatc ccggcgcgga caacggcgtc gtggcggcga accgggccag
    62881 catcgcccgg gcgctcgatc tctcgaacca catcaacgcc tctctttcgt tcaactacaa
    62941 ccgcgccaac ctggaggccg gggacaccat gctggtggaa gtgtcgacca acggcggcgc
    63001 cacctacacc accgtgggga cctttccggg aagcaccgcc gtcggagtgc agaacgtcag
    63061 cctggccggc tacgaatcgg ccaatacgtt gatccggttc cgcctcagca ccgacttcgc
    63121 cacgaacgaa acggtgacga tcgacaacgt caccattacc gccgagccca ccggctacac
    63181 gaataccttt accgaggcgg tgacctatgt cgccggcgcc ggcggcgccg cgatcgcggc
    63241 cgcggacacc ctcattaccg atccgagcgg cggcaacctg gccggcgcga tgattaggat
    63301 cgcgaccaat taccagagcg gcgccgacct tctcgagttc accaaccagg gcgggatcac
    63361 cggcgtgttc aacgcggcga ccggcacgct caccctgacc ggcaacgcca cggcggctgt
    63421 ctacacgacg gcgatcgaat cgatccgttt caacaactcc agcggcgcgc ccagcaccag
    63481 cccgcgaacc atcaccgtcg tcgtgaccga taccaccggc gcggcgagca atacggcaaa
    63541 aagcacgatc aacgtcgtcg cgaccaacga tgccccggtc ggggttaacg acgcgtcgac
    63601 cgcgatcgag gccggcggcg tggcgaacgc cacgccggga ctcaatccga cgggcaacgt
    63661 gctgaccaac gacacggacg tggacgctgg cgataccaag accgtgtcgg cgatcaccgg
    63721 cggcacggtc ggcgtagcga aggcaggcag ctacggctcg atcgtgctca acgccaacgg
    63781 cagctacacg tataccgtgg acaactcgaa cgccgcggtc gaggcgctgc gcagtgcagc
    63841 cagtgagttg tacgacacgt tcacctatac ggtacgcgac acggccggcc ttaccagcac
    63901 ggcgaccctg acgatcacca ttcagggcgc gaacgatacg gcgacgatcg gtacgcccag
    63961 cgtggcggtg gtgaccgagg acgtcggcgt ctccggcggc aacctcagcg caaccggctc
    64021 gatcgcaatc agcgacgtgg acgaccacgg cgcgatcttc agcaccatcg tggtgccggt
    64081 cggcagcccg cttggcgcgc tggtgctggc cgcggacggg acctatacct acacggtgtc
    64141 gaacgccgcg gtccagagcc tgggcgccgg ccagaccgcg atcgacacgt tcatggtgac
    64201 gagcgccgac ggcacgacgc agaacgtctc cttcacgatc aacggcgcga acgatgctcc
    64261 ggtcaacgcc gtcccgggcg cgcaggcggt gaacgaggac accgcgctct cgatcggcgg
    64321 cgtgagcgtc aacgacatag agggcaatct ctcgaccacc cgcctcacgg taaccaacgg
    64381 tgccgtgacg gtgagcctgg cgggcggtgc gacgatcagc gcaggcgcca atggcagctc
    64441 gacgctgacc ctgtcgggca cgcaggcgca gatcaatgcc gcgctcgcgt cgatcagcta
    64501 ccagggcaac ctgaacttca acggcggcga cacgctgacg gtgctgtcga ccgacagcaa
    64561 cggtgccacc gactccgatg cggtcgcgat caccgtcaac ccggtgaacg acgggccggt
    64621 caacacggtg ccggccgcgc agagcgtgaa cgaagacacg gcactctcga tcgccggcgt
    64681 cagcgtgaac gacgtcgacg gcaacctgtc gaccacccgc ctcacggtaa ccaacggtgc
    64741 ggtgacggtg agcctggcgg gcggggcgac gatcagtgcc ggcgcgaacg gctcgtcgac
    64801 cctgacgctc tcgggcacgc aggcgcagat caacgccgcg ctt
//
LOCUS       DATBSZ010000274         8786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2102714, whole genome shotgun sequence.
ACCESSION   DATBSZ010000274 DATBSZ010000000
VERSION     DATBSZ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8786
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2102714"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VIG70_17935"
     CDS             <1..314
                     /locus_tag="VIG70_17935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8252477.1"
                     /translation="KLSDRYDIVSTNTSANALKLAREVQPDVILCDLDMPEMDGAEVS
                     EALRADDELREIPLLFLTALASSGDLLRMRKQLGGRPAVSKDAPLQQLVARIEALLKT
                     G"
     gene            complement(318..1064)
                     /locus_tag="VIG70_17940"
     CDS             complement(318..1064)
                     /locus_tag="VIG70_17940"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8252478.1"
                     /translation="MQEWIVVLLAAGMLVAAFGYASVGHGGASAYIAAMALVGIAPAE
                     MRPIALLLNVLVSSLGTWKFYRAGYFRWRLFWPFAVVSIPMAYLGGAITLPGQAYKVL
                     VGLGLLYAAWQLWRSGKSGEEMREVREPPLGWAMAIGAAMGLLAGLTGVGGGIFLSPL
                     LLMLGWAGTKQTSAVAAPFILVNSIAGLAAVFVAKGAVLPSYVWILGAAVLAGGWLGA
                     EYGSRRFANPVVRRVLAIVLALAGAKMISV"
     gene            complement(1077..1421)
                     /locus_tag="VIG70_17945"
     CDS             complement(1077..1421)
                     /locus_tag="VIG70_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8252479.1"
                     /translation="MARYRGLTLRILAGRSPAMGPGKAELIERIAGTGSISAAARAMG
                     MSYRRAWQLAEALNKDYRERVVSTAAGGARGGGARVTPFGLRVLAEYRAMEEKASAAI
                     ASELRRFKRYIP"
     gene            complement(1421..2233)
                     /locus_tag="VIG70_17950"
     CDS             complement(1421..2233)
                     /locus_tag="VIG70_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8252480.1"
                     /translation="MGLIRALLAALVCFSCAVEAQSRYITVASTTSTEQSGLFKHLLP
                     IFEKRTGIQVRVVALGTGQALDMGRRGDADVVFVHARPLEEKFVADGYGVRRQEVMYN
                     DFVLIGPKSDPAKVVGGKDAVQAFQKIKEAKAPFVSRGDRSGTHFAELELWKAAGIDI
                     ARDKESWYRETGQGMGPALNTASGMNAYVLADRGTWLSFKNRGELAIAVEGDRRLFNQ
                     YGIILVNPQKHLGVKKDLGQAFIDWIISSEGQEAIAAYKIGGEQLFFPNAGG"
     gene            complement(2224..2916)
                     /locus_tag="VIG70_17955"
     CDS             complement(2224..2916)
                     /locus_tag="VIG70_17955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEY8252481.1"
                     /translation="MILPLRLQNVSFRDILKPLSLEIEAGPSTIILGANGAGKSVLMR
                     IMHGLLAPSTGSVSWQGNDPGRRQAMVFQRPVMLRRSAYANVAYALKVAGMPAKERAR
                     LAAEALEEVGLRQVTHRPARVLSGGEQQRLALARAWALHPEVLFLDEPTANLDPGATR
                     EIETIIKAFDASGTKIVMSTHNLGQARRLGDEVLYLHEGRVVERAPIERFFAQPSAPE
                     ARAFIKGELPWV"
     gene            complement(2913..3572)
                     /locus_tag="VIG70_17960"
     CDS             complement(2913..3572)
                     /locus_tag="VIG70_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8252482.1"
                     /translation="MLEDPRLWQIVALSLAVSLSAVAIATLVGMPLGAAIAVGRFPGR
                     QAVIVLLNALMGLPPVVVGLAIYLMLSRAGPLGELGLLFTPQAMVIAQTVLVLPIIAA
                     LARQSVEDAWREYEEQLRSLGVHGLRGALTVLWDIRFSLLTAVLAGLGRASAEVGAVM
                     IVGGNIDGVTRVMTTTIALETSKGDLPLALGLGVVLIAIVLALNAAAYLVKEAAQRRF
                     G"
     gene            3653..4663
                     /locus_tag="VIG70_17965"
     CDS             3653..4663
                     /locus_tag="VIG70_17965"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8252483.1"
                     /translation="MHTSFTVDFDHAWLAGRDFPRERGRTLLRLLDELARAGSLREAA
                     RKAGMSYRSAWGALGDGARLFGAPLVDMQRGRRASLSPLGRKVLAADERVREGLGEHF
                     ERLRAEIPAMLADALPGTRPRLTLHASHDLALAELGALCRRALELEVVIRGADDSLAA
                     LARGECDVAGFHVADALPRAAAAAAALGRWLDPRKHTLIHFVAREQGLIARPGSRIRG
                     VHDLARPGVRFIHRQSRGADGDELSVAEAVAAGRADAGFGLRADATRHKLAFAPLAME
                     RYFFACAKAALRGAALKLLVETLQRPDFAARVARLPGYDASQSGRREPLDAALTWVAR
                     KR"
     gene            4687..5781
                     /locus_tag="VIG70_17970"
     CDS             4687..5781
                     /locus_tag="VIG70_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEY8252484.1"
                     /translation="MRRRSFLQAGAAASVLSTLPRLGLAQQLPYDPRPSGWRTFEVTT
                     RVEILKPAGGSRAWVPLPSIEGDYQKLIGNTWSGNGSTRVARDGKYGAAMVMSEWSAA
                     EKAPVLEVVSTFSTQNRAVDFSKRNPAIKLDTASARFSVAPTELIPTDGIVRKTALEI
                     TRGKTSELDKARGIYEWIVDNTFRNPKTRGCGVGDIKAMLESGDLSGKCADLNALYVG
                     LARAVNLPARDVYGLRVARSEFGYRSLGVGSPNVTRAQHCRAEVFLTGYGWVPVDPAD
                     VRKVVLEEKAQPTTLADPVVPPVRAKLFGAWEMNWLAYNEAHDVKLPGSTGPAVGFLM
                     YPQAETGSERLDSLDPDNFKYSITAREIKA"
     gene            5873..6208
                     /locus_tag="VIG70_17975"
     CDS             5873..6208
                     /locus_tag="VIG70_17975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HEY8252485.1"
                     /translation="MDTYYRPEHLPKFSSIAQGNQKLADKFFDYYGEVFKAGALSARE
                     KALIALAVSHVVQCPYCIDAYSKESLQQGADLEQMTEAVHVAAAIRGGASLVHGVQML
                     EHVQRQVMA"
     gene            6351..7286
                     /gene="arsS"
                     /locus_tag="VIG70_17980"
     CDS             6351..7286
                     /gene="arsS"
                     /locus_tag="VIG70_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_274671337.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEY8252486.1"
                     /translation="MFREKLTPAGIDIFQINVGKLCNMTCRHCHVDAGPDRTAEMMSR
                     ETVDLCLAALDKTTAKVVDITGGAPELNPYFRYLVEQCVARGKHVMDRCNLTILLTGP
                     CRDLPEWFASRGVEVVCSLPHYRQRNTDLQRGTGTFEKSIEALRRLNAAGYGRGDPRL
                     RLTLVHNPAGAYLAGDQKSMEREWKEGLKANHGVTFDALIGLNNMPISRFLEWLETSG
                     NLQAYMELLVNAYNPATVPGLMCRNTLSVGWDGRIYDCDFNQMLELDARHADGRPAHI
                     RDFDPQQLATRAIVTARHCFGCTAGAGSSCGGAIA"
     gene            7283..8305
                     /locus_tag="VIG70_17985"
     CDS             7283..8305
                     /locus_tag="VIG70_17985"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8252487.1"
                     /translation="MTRSLIFISALFPAVCSSQGAAVDATGREIRPPAKVERVYAAGP
                     PASMLVFAIAPGKLTGWTRAMRPNEAQFLPERYAQLPELGRLTGRGNTANVEVVLNAR
                     TDLIVDVGSTGETLASLATQVQQQTRIPYALFDGRIESTPAALRALGRLMGDEREAER
                     LAAWYEKELTQAKQRVARAGAHPVVYYGRSPSGLQTGGKGSINIEVLDFLGARNAAAE
                     ARAGLATVSLEQVLLWDPEVILTTDPNYWKSVWTDPRWRAVKAVAAKRVYLSPHLPFG
                     WFDFPPGANRLLGVWWAGKLLYPQTFDDVDLRAKVVEFHRLFYHREPSAAQLDSLLSE
                     PGVLPR"
     gene            8302..>8786
                     /locus_tag="VIG70_17990"
     CDS             8302..>8786
                     /locus_tag="VIG70_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEY8252488.1"
                     /translation="MNARALSYVLAAGALVALTALAFAVGRYPVSLAEIFSVLFGQPV
                     SANVETVVLQVRGPRVLAALLVGAALAAAGTAYQGMFRNPLVSPDILGVSTGAALGAV
                     LGIFLSQGILFIQIFAFAGGLAAVGLVYWIGSRLRGHDPLLALVLTGVVIGTLLGSAI
                     AL"
BASE COUNT         1436 a         3107 c         2886 g         1357 t
ORIGIN      
        1 cgaagctctc cgatcgctac gacatcgtct ccaccaacac ttccgccaac gcgctcaagc
       61 tggcgcgcga agtccagccc gacgtgatcc tctgcgacct ggacatgccg gagatggacg
      121 gcgccgaggt ctccgaggcc ctgcgcgccg acgacgagct gcgcgaaatc ccgctgctct
      181 tcctcaccgc cctcgcctcg tcgggcgatc tcctgcgcat gcgcaagcag ctcggcgggc
      241 gcccggcggt ctcgaaggac gcgccgctcc agcagctcgt cgctcgcatc gaggcgctgc
      301 tgaagacagg ctagtcttca taccgagatc atcttcgcgc cggccagcgc cagcacgatc
      361 gcgaggacgc ggcgcaccac cggattagcg aaacgccggc tgccgtactc ggcacccagc
      421 cagccgcccg ccagcaccgc agccccgagg atccagacgt acgagggcaa aacagcgccc
      481 ttcgcgacga aaaccgcggc cagcccggcg atcgagttca ccaggataaa gggcgcggcg
      541 acggcggaag tctgcttggt tccggcccag ccgagcatca gcagcagggg gctgagaaaa
      601 attccgccgc ccacgccggt caacccggcg agcagaccca tggccgcgcc gatcgccatc
      661 gcccagccga gcggcggctc gcgcacctcg cgcatctctt cgccactctt gccgctgcgc
      721 cagagttgcc aggcggcgta aagcagaccc aggccgacga ggaccttgta ggcctgcccg
      781 ggcagcgtga tggcgccgcc gaggtaggcc atcggaatcg acaccacggc gaacggccag
      841 aacagccgcc agcggaaata ccccgcccga tagaacttcc acgtgccgag cgaagagacc
      901 aggacattga ggagaagcgc gatcggccgc atctcggcgg gcgcaatgcc cacgagcgcc
      961 atggccgcga tgtaggcaga ggcaccgcca tgcccgaccg aggcatagcc gaaggccgcg
     1021 accagcatgc ccgcggcgag cagcacgacg atccattcct gcacctgcgc tatatatcac
     1081 ggaatatagc gcttgaatcg ccgcagttcg ctcgcaatcg cagccgaggc cttttcctcc
     1141 atcgcgcgat actcggcgag cacgcgcaag ccaaaaggcg tgacccgcgc gccgccgccg
     1201 cgcgcgccgc cggcggccgt cgagaccacg cgctcgcgat agtccttgtt gagcgcctcg
     1261 gccagctgcc aggcgcgccg gtaggacatt cccatcgccc gcgccgcggc cgagatcgag
     1321 ccggtgccgg caatgcgctc gatgagctct gccttgcccg ggcccattgc cggcgaccgg
     1381 ccggcaagga tgcgcaaagt gaggccgcga tagcgcgcca tcagccgcct gcgttgggga
     1441 agaacaattg ctcgccgccg atcttgtacg cggcgatcgc ctcctgccct tcgctggaga
     1501 tgatccagtc gatgaatgcc tgccccagat cctttttcac gccgaggtgc ttttgcggat
     1561 tcacgagaat gatgccgtac tggttgaaga gccgccggtc gccctccacc gcgatcgcga
     1621 gctcgccgcg gttcttgaac gagagccagg tgccccgatc ggcaagcaca taggcgttca
     1681 tgccggaggc ggtgttcagc gctggcccca tgccctgccc ggtctcgcgg taccagcttt
     1741 ccttgtcgcg ggcgatatcg atcccggcag ccttccacag ctcgagctca gcgaagtgcg
     1801 tgccgctgcg atcgccgcgg gacacgaagg gcgccttggc ttccttgatc ttctgaaaag
     1861 cctgaaccgc atccttgccg ccgacgacct tggccggatc cgacttcggc ccgatcagga
     1921 cgaaatcgtt gtacatcacc tcctggcggc gcacgccgta gccatccgcc acgaacttct
     1981 cctcaagcgg cctggcgtgc acgaacacca cgtcggcgtc gccgcggcgt cccatgtcga
     2041 gcgcctggcc ggtgccgagc gcgaccacgc gcacctgaat cccggttctc ttctcgaaga
     2101 tgggcagcag gtgcttgaac agacccgact gctcggtcga ggtggtcgac gcgaccgtga
     2161 tgtagcgtga ctgcgcttcg acggcgcacg agaagcagac cagcgccgca agaagtgctc
     2221 ggatcagacc catggcagtt ctcctttgat gaacgcgcgc gcttccgggg ccgacggctg
     2281 cgcgaagaat cgctcgatcg gcgcgcgctc cactacgcgg ccctcgtgga gatacaggac
     2341 ctcgtcgccc aggcgccgcg cctggccgag gttgtgagtg gacatgacga tcttggtgcc
     2401 ggaagcgtcg aatgccttga tgatcgtttc aatttcgcgc gtcgcccccg gatcgagatt
     2461 ggcggtcggc tcgtcgagga acaggacttc cgggtgcagc gcccaggccc tcgccagggc
     2521 gagccgctgc tgctcgcctc ccgaaagaac gcgcgcgggc cgatgcgtaa cctggcgcag
     2581 tccgacttcc tcgagcgctt cggccgccag gcgcgcgcgc tccttcgccg gcatcccggc
     2641 aactttgagc gcgtaggcca cgttggcgta ggcggagcgg cgcagcatca ccggccgctg
     2701 aaacaccatc gcctgcctgc gccccgggtc gttcccttgc caggaaacgc tgcccgtcga
     2761 aggcgcgagc aggccgtgca tgatgcgcat gagcacgctt ttcccggcgc cgttcgcgcc
     2821 gaggatgatc gtgctcgggc ccgcttcgat ctccagggac agcggcttga gaatgtcgcg
     2881 gaacgagacg ttctgcagcc tgagcggcag gatcatccga accggcgttg cgccgcctcc
     2941 ttgacaagat aagcggctgc gttcagcgcc agcacgatcg cgatcagaac cacaccgagg
     3001 ccgagcgcga gcggcaggtc gcccttggag gtctcgagcg cgatggtggt cgtcatcacg
     3061 cgcgtgacgc cgtcgatatt gccgccgacg atcatcactg cgcccacttc ggcgctggcg
     3121 cgcccgagcc cagccagtac cgcggtgagc agggagaagc ggatgtccca gagcacggtg
     3181 agcgcgccgc gcagcccgtg cacgccgagg gaccgcaact gttcttcgta ctcccgccac
     3241 gcgtcctcga ccgactgccg ggcaagcgcg gcgatgatgg gcagcaccag cacggtctgc
     3301 gcgatgacca tcgcctgcgg cgtgaacagc agccccagct cgcccagcgg gccggcgcgc
     3361 gacagcatga gatagatggc gagacccacc accaccggcg gcaggcccat cagggcattc
     3421 agcagcacga tgaccgcctg gcgccccggg aagcgtccca ccgcgatggc ggcgccgagc
     3481 ggcatgccga cgagcgtggc gatcgccacc gccgacaggc tcacggccag gctcaaggcc
     3541 acgatctgcc aaagccgggg gtcttcaagc atcgttatgt ataagattgc atacctgatg
     3601 gcatccgaca aactctaccc gcgctttcct gaagctctaa ataggcacgt tcatgcatac
     3661 ttcgttcacc gttgacttcg accacgcctg gctggcgggg cgcgacttcc cgcgcgagcg
     3721 cggccgtacc ctgctgcggc tgcttgacga gctcgcgcgc gccggttcgt tacgcgaggc
     3781 cgcgcgcaag gcgggcatgt cgtatcgctc ggcgtggggc gcgctcggcg acggcgcccg
     3841 gttgttcggc gccccgctgg tggacatgca gcgcgggcgc cgcgccagcc tcagccccct
     3901 cgggcgcaaa gtgctcgccg ccgacgagcg cgtgcgtgaa gggctcggcg agcacttcga
     3961 gcgcctgcgc gccgagatcc cggcgatgct cgccgacgcg ctgcccggca cgcggccgcg
     4021 cctgaccctg cacgcgagcc acgacctcgc gctcgcggag ctgggcgcgc tgtgcaggcg
     4081 cgcgctggag ctggaggtgg tgatccgcgg cgcggacgac agcctcgccg cgctggcgcg
     4141 cggcgagtgc gacgtggcgg ggtttcacgt cgccgatgcg ctgccgaggg ccgcggccgc
     4201 cgccgccgcc cttggccgct ggctcgaccc gcgcaagcac accctgatcc acttcgtcgc
     4261 gcgggagcag gggctgatcg cccgcccggg atcgcgcatc cgcggcgttc acgacctcgc
     4321 gcgcccgggg gtgcgcttca tccaccggca atcgcgcggc gccgacggcg acgagctgtc
     4381 ggtcgccgaa gcggtcgccg ccggccgcgc cgacgccggc ttcggcctgc gcgccgacgc
     4441 tacgcgccac aagcttgcct ttgcgccgct cgcaatggag cgctacttct tcgcctgcgc
     4501 gaaggccgcg ctgcgcggcg cggcgctgaa attgctggtg gagactctgc agcgtccgga
     4561 tttcgccgcg cgcgtcgccc gcctgcccgg ctacgacgcc tcgcaatccg gtcggcgcga
     4621 accgctcgat gcggcgctca cctgggttgc gcggaagcga taacatcggc tattccagga
     4681 ggacacatgc gcagacgatc gttcctgcaa gccggcgccg cggcgtcggt gctaagcacc
     4741 cttccccgcc tcggcctcgc ccagcagctt ccctacgacc cgcggccgtc gggctggcgc
     4801 accttcgagg tcaccactcg cgtcgaaatc ctgaagcccg ccggcggctc gcgggcctgg
     4861 gttccccttc cctcgatcga gggcgactac cagaaattga tcggcaacac ctggtcaggc
     4921 aatggttcga cccgcgtcgc gcgcgacggc aagtacggcg ccgcgatggt gatgtcggag
     4981 tggagcgccg cggagaaggc tccggtgctc gaggtagtga gcaccttttc cacacagaac
     5041 cgcgccgtgg acttcagcaa gcgcaatccg gcgatcaagc tcgatacggc cagcgctcga
     5101 ttcagcgtcg cgcccaccga gctcattccc accgacggca tcgtgcgcaa gaccgcgctc
     5161 gagatcaccc gcggcaagac ctcggagctc gacaaggcgc gcggcatcta cgagtggatc
     5221 gtcgacaaca cgtttcgcaa tcccaagacg cgcggctgcg gcgtgggcga catcaaggcg
     5281 atgctcgagt cgggggacct gagcggcaag tgcgccgacc tcaatgcgct ctacgtcggc
     5341 ctggcgcgag cggtcaacct cccggcgcgc gacgtctatg gcctgcgcgt ggcccgcagc
     5401 gaattcggtt accgcagcct gggcgtcggc agccccaatg tgacgcgcgc gcagcactgc
     5461 cgcgccgagg tgttcctcac ggggtacggc tgggtcccgg tcgacccggc cgacgtacgc
     5521 aaggtggtgc tggaagagaa agcgcagccg actacgctcg ccgacccggt ggtgccgccg
     5581 gtgcgcgcca agctttttgg cgcctgggag atgaactggc tcgcctacaa cgaggcgcat
     5641 gacgtcaagc tgcccggctc caccggtcca gccgtcggct tcctgatgta cccgcaggcg
     5701 gagaccggaa gcgagcgcct cgacagcctc gacccggaca acttcaagta cagcatcacg
     5761 gcgcgggaaa taaaggccta gggcgctgcg ttcactggga aagcccgccc gcgcggcggg
     5821 cttttttgtt cttggcgtaa ggtggctggc ccacaatcga caaaggcaca ccatggatac
     5881 ctactacaga cccgagcacc tgccgaagtt ctccagcatc gcgcagggca accagaagct
     5941 cgccgacaag ttcttcgact actacggcga agtgttcaag gccggcgccc tgtcggcgcg
     6001 cgaaaaggcg ctgatcgctc tcgccgtgtc gcacgtcgtt caatgccctt attgcatcga
     6061 tgcctactcc aaggagtccc tgcagcaggg cgccgacctc gagcagatga ccgaagcggt
     6121 gcacgtcgcc gcggcgatcc ggggcggcgc atccctggtg cacggcgtgc agatgctcga
     6181 gcacgtgcag cgacaggtga tggcctagcg tgggtgcacc ggaggcccaa aagctcagcg
     6241 ggcctcgcgc gacgacttcc ctcgtctcgc gtgcttcgcc gctcgtctcg acggcgcagc
     6301 agctcaagac gcttcgccaa ctggatcttt ccaagttgcc gaatcaaggg gtgttccgcg
     6361 aaaagctgac cccggccggc atcgacatct tccagatcaa cgtcggcaag ctctgcaaca
     6421 tgacctgccg gcattgccat gtggacgcag ggcccgaccg caccgcagag atgatgagcc
     6481 gcgaaacggt ggacctgtgc ctcgcggcgc tcgacaagac cacggcgaaa gtcgtggaca
     6541 ttaccggcgg cgccccggag ctcaacccgt acttccgtta tctcgttgag caatgcgtgg
     6601 cgcgcggcaa gcacgtgatg gatcgctgca acctgacgat cctgctgacc ggcccgtgtc
     6661 gcgatctgcc cgaatggttc gccagccgcg gcgtggaggt cgtgtgctcg ctgccgcact
     6721 accgccagcg caacaccgac ctgcagcgcg gcaccggcac cttcgagaag tcgatcgagg
     6781 cgctcaggcg cctcaacgcc gccggctacg gccgcggcga cccccgcctg cgactcacgc
     6841 tcgtgcacaa cccggctggg gcttacctcg ccggcgacca gaaatcgatg gaaagagagt
     6901 ggaaggaagg cttgaaagcg aaccacggcg ttaccttcga cgcgctgatc gggctcaaca
     6961 acatgccgat ctcgcgcttt ctcgagtggc tcgagacctc ggggaacctg caggcctaca
     7021 tggagctgct ggtcaacgcc tataaccccg caaccgtgcc gggcctgatg tgccgcaaca
     7081 cgctctcggt cggctgggat gggcgcatct acgactgcga cttcaaccag atgctcgagc
     7141 tcgacgcgcg tcatgccgac gggcggcccg cgcacatccg cgacttcgat ccgcagcagc
     7201 tcgccactcg cgccatcgtc accgcgcgcc actgctttgg ctgcaccgcc ggcgccggca
     7261 gctcctgcgg cggcgccatc gcgtgacgcg cagcctcatt ttcatctcgg ctctctttcc
     7321 tgcagtctgt tcttctcagg gcgctgcggt cgacgcgacc ggccgggaaa tccggccgcc
     7381 cgcgaaagtc gagcgcgtct acgccgccgg gccgccggcg agcatgctgg tgttcgcgat
     7441 cgcgcccggc aagctcaccg gatggacgcg cgcgatgcgg ccgaacgagg cgcagttcct
     7501 tccggagcgc tatgcgcagc tgcccgagct cggccggctc accggccgcg gcaacaccgc
     7561 gaacgtggag gtggtgctga atgcgaggac cgatttgatc gtggatgtcg gctcgaccgg
     7621 cgaaaccctc gcatcgctcg ccacgcaggt gcagcagcag acgcgcatcc cgtacgcgct
     7681 gttcgacggg cgcatcgagt cgacgccggc ggcgctgcgc gcgctcggcc ggctgatggg
     7741 agacgagcgc gaggctgagc gcctcgctgc ttggtacgag aaagagctga cgcaagccaa
     7801 gcagcgtgtc gcgcgcgccg gcgctcaccc cgtggtctac tacggccgca gcccgagcgg
     7861 cctgcaaacc ggaggaaagg ggtcgatcaa catcgaggtg ctcgatttcc tcggcgcacg
     7921 caacgccgcg gccgaggcgc gcgccgggct cgcgaccgtg tcgctcgagc aagtgctgct
     7981 gtgggacccg gaggtgatcc tcaccaccga cccgaactac tggaaatcgg tctggaccga
     8041 cccgcgctgg cgcgcggtga aggcggtggc ggcgaagcgc gtctatctgt cgccgcacct
     8101 gccgttcggc tggttcgatt tcccgcccgg tgccaaccgc ctgctcggcg tgtggtgggc
     8161 ggggaagctg ctctatccgc agacgttcga cgacgtcgac ctgcgcgcca aagtcgtcga
     8221 gttccaccgc ctgttctatc accgcgagcc gagcgcggca cagctggatt ccttgttgag
     8281 cgagccgggc gtgctcccgc ggtgaacgca cgcgcgttga gctacgtgct ggcggcgggg
     8341 gcgctcgtcg cgctgacggc tctcgctttc gccgtcggcc gctacccggt ctcgctggcc
     8401 gagattttct ccgtgctgtt cggacagccg gtcagcgcca atgtcgagac cgtggtgctc
     8461 caggtccgcg gcccgcgcgt gctcgccgcg ctgctggtgg gggcggcgct cgccgccgcg
     8521 ggaaccgcgt accagggaat gttccgcaac ccgctcgtgt cgcccgacat cctgggcgtg
     8581 tcgaccggcg cggcgctcgg cgccgtgctc gggatttttc tttcgcaggg gatcttgttc
     8641 atccagatct tcgccttcgc cggcgggctc gccgcggtcg ggctggtcta ctggatcggc
     8701 tcgcgcttgc gcggccacga cccgctgctc gcgctggtgc tcaccggcgt ggtgatcggg
     8761 accctgctcg gctcggcgat cgcgct
//
LOCUS       DATBSZ010000275         7043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_54569, whole genome shotgun sequence.
ACCESSION   DATBSZ010000275 DATBSZ010000000
VERSION     DATBSZ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54569"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VIG70_17995"
     CDS             <1..1119
                     /locus_tag="VIG70_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HEY8252489.1"
                     /translation="NKKYGLYGPVPKKVLLQNIRKHPDAQALILTSCTYDGLRYDLAP
                     IVAAAHERGIKVIIDEAWYGFARFHPYFRPTALEAGADYATQSTHKVLSAFSQASMIH
                     VNDPGFREHLFRENFNMHTSTSPQYGLIASLDIARKQAVMEGYKLLTRTLELATEVRK
                     LVNSTRVFRVLELEDLLPPEVRNDGIQLDPTKVTIDISGCGYTADDLQKALFERFNIQ
                     VEKSTFNTLTLLLTIGTTRSKVSRLYDALMRIAREQRAPRRLVQSPEIPPFTRLKFLP
                     RDAYYCGGELVPVFDERERVNRKLISRVAADQIVPYPPGIPVLVPGQRITKSIVDYLG
                     ALLHSHKRMEMHGVVHEGYLPCIRVLLPAEERALTRIA"
     gene            1196..2386
                     /locus_tag="VIG70_18000"
     CDS             1196..2386
                     /locus_tag="VIG70_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8252490.1"
                     /translation="MSHVMNTYARQPVAFVRGEGVWLWDEAGKKYLDALAGIAVNTLG
                     YGHPKLTAALSKRIQSGVLHTSNLWRIPDQEKAADRIAEITGLDEVFFCNSGLEANEA
                     AIKVARKYGHDRGVAEPAIVVMEKAFHGRSLATLSATGSRKVQAGFEPLVSGFVRVPL
                     NDLEAVRQVAEHNKNVVAVFVEPIQGEGGINVSRLDYLRGLKEICERKEWLFMSDEVQ
                     CGLGRTGKWFVYQHAGFLPDVVPLAKGLGSGVPVGACVVGGRAKGVFKPGNHGSTFGG
                     NPLAMTGVVATIDTIKEEGLLANATRVGDVIRAGLSQIQGISEVRGMGLMIGIDAGVA
                     CGELVRQGLEAGLVINVTADNVIRLLPPLVMTEAEGRLVVERLAPLVRAFLERSSQPK
                     AAAH"
     gene            2394..3296
                     /gene="argF"
                     /locus_tag="VIG70_18005"
     CDS             2394..3296
                     /gene="argF"
                     /locus_tag="VIG70_18005"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY8252491.1"
                     /translation="MAVRHFLQFRDFSLAELEHVFTRTRWIKDKFKRYERYWPLEDRT
                     LAMIFEKPSTRTRMSFEAGMHQLGGAAIYLFTRDSQLGRGEPVEDAVQVISRMCDVIM
                     LRTFEQDIIQRFAGSSRVPVINGLTNEYHPCQILADVFTYVEHRGAIRGKTVAWIGDS
                     NNVCNTWLQAAALFDFKLHVSTPPGYEIEKERINRNCEVFDEPMKAAKGADLVTTDVW
                     TSMGFEAENEERRRDFQDWQVDAEMMRAAKKDALFMHCLPAHRGEEVAAEVIDGKQSV
                     VWDEAENRLHTQKALLEFLVLGKK"
     gene            3298..3912
                     /locus_tag="VIG70_18010"
     CDS             3298..3912
                     /locus_tag="VIG70_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY8252492.1"
                     /translation="MFGVADYWAFVVAVIVFLAIPGVGNLAIITSTSKGRIVGGLAAT
                     LGVIVGDQVLMWLAVAGVAAVLTANPALFGVVQYAGAIYLAYLGIRMVLAKPGDAPVL
                     EMQPRRFFRQAMLITLLNPKAIVFYMAFFPLFVDPARHQGLPTFGVMALTIAVLTFLY
                     CVIVISITHFAADRMRASPRVAVWLNRIAGTMLLGFGVKLAVGK"
     gene            complement(3913..5253)
                     /gene="argG"
                     /locus_tag="VIG70_18015"
     CDS             complement(3913..5253)
                     /gene="argG"
                     /locus_tag="VIG70_18015"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY8252493.1"
                     /translation="MGTILGTLPKGEKIGIAFSGGLDTSAAVHWMRSKGAIPYAYTAH
                     LGQPDESDYADIPRKALSYGAQKARLVECRPQLVAEGIAALQCGAFHISTGGATYFNT
                     TPLGRAITGTMLVVAMREDGVGVWSDGSTYKGNDIERFYRYGLLANPDLRIYKPWLDQ
                     RFIDELGGRREMSAYMASAGFDYKMSAEKAYSTDSNLLGATHEAKDLEFLDKGIGIVQ
                     PIMGVAFWRDDVKVKAERVSVEFAQGQPVALNGQRFSDCVKMMEDANAIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRTNGRRLGRLLYQG
                     RWFDPQAMMLRETAQRWVAKAVTGEVTLELRRGNDYSILDTKSPNLTYKPERLTMEKG
                     EEVFFTPGDRIGQLSMRNLDIADTRDKLRVYGEAGLLGDGSGVPLLGPEKPKKR"
     gene            5303..5968
                     /locus_tag="VIG70_18020"
     CDS             5303..5968
                     /locus_tag="VIG70_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252494.1"
                     /translation="MRALASVLAALLAASSQPCAAAPFAVQVGEARIGLDAPPGFADT
                     TATGSPRLQELGEATTSASNRVLLFAVSDADLRRFTLGDPLELRRYMVVVTPRAMERE
                     RMSESAFQAFAGGSLRELGPVSGATDYQKYLDSRPVGAVSTLAELRKDPDAVAVLQGS
                     RSKGGFLERSKFVLSSTSVVLLRGKALTLSIFTQYDDPADLEWIRNATARWIDELKRL
                     NSR"
     gene            5983..6468
                     /locus_tag="VIG70_18025"
     CDS             5983..6468
                     /locus_tag="VIG70_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY8252495.1"
                     /translation="MPSFDIVSQVDQQEVKNAVEQTNKEVTNRFDFKGSDARVEQSEL
                     VLTVYADDEFKLGQVLDVLRTRLAKRGVDVRSLEPGAVEKISGDKVKRPVTVKVGVPQ
                     EKGKQIQKLVKDSKLKVAASIQGDAVRISGAKKDELQSAIQLVRKSVADLPLQFINFR
                     D"
     gene            complement(6491..>7043)
                     /locus_tag="VIG70_18030"
     CDS             complement(6491..>7043)
                     /locus_tag="VIG70_18030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEY8252496.1"
                     /translation="MGGPMSVNDDLPWIRPLLELVREAVRKDVPALGHCLGGQLMSKA
                     LGGEVTRARVKEIGWGEVEVADNGVARAWFGAPAFESFHWHGETFSIPPGATRVLEGE
                     HCANQAFALGRHFAMQCHVEMTPELVRTWLSTGSEEIREHKKSPAVQPPAEIEKDLAP
                     RLDRLHEVADKIYDRWSEGLSRG"
BASE COUNT         1146 a         2397 c         2309 g         1191 t
ORIGIN      
        1 aacaagaagt acggcctgta cggcccggtg cccaagaaag ttcttctcca gaacatcagg
       61 aaacatccgg atgcgcaggc gctgatcctc acctcgtgca cctacgacgg gctgcgctac
      121 gacctcgcgc cgatcgtcgc cgccgcgcac gagcgcggca tcaaggtcat catcgacgag
      181 gcctggtacg gcttcgcccg cttccatccc tacttccggc cgaccgcgct cgaagccggc
      241 gccgactacg ccacccagtc gacgcacaag gtgctgagcg ccttctcgca ggcctcgatg
      301 atccacgtca acgacccggg cttccgcgag cacctgttcc gcgagaactt caacatgcac
      361 acctcgacca gcccgcagta cgggctgatc gcgagcctcg acatcgcgcg caagcaggcg
      421 gtgatggagg gctacaagct cctgacgcgc accctcgagc tcgccaccga ggtgcgcaag
      481 ctcgtcaact cgacaagagt gttccgcgtg ctggagctcg aggacctgct gccgcccgag
      541 gtgcgcaacg acggcatcca gctcgatccg accaaggtga cgatcgacat ctcgggctgc
      601 ggctacacgg ccgacgacct gcagaaggcg ctgttcgagc gcttcaacat ccaggtcgag
      661 aagtcgacct tcaacaccct gacgctcctg ctgaccatcg gcaccacgcg ctcgaaggtt
      721 tcccgcctgt acgacgcgtt gatgcgcata gcgcgcgagc agcgcgcgcc gcggcggctg
      781 gtgcagagcc cggaaattcc gcccttcacg cgcctcaagt tcctgccgcg ggatgcctac
      841 tattgcggcg gcgagctggt gccggtgttc gacgagcgcg agcgggtcaa ccgcaagctg
      901 atcagccgcg tcgccgccga ccagatcgtg ccctacccgc ccggcatccc ggtgctggtg
      961 ccgggacaga ggatcacgaa aagcatcgtc gactacctcg gcgccctgct ccactcgcac
     1021 aagcgcatgg aaatgcatgg cgtcgtacac gagggctatc tgccgtgcat ccgggtgctg
     1081 ctgccggccg aagagcgtgc gctgacgcgc atcgcctagc ctaccctcgc cagcggtgct
     1141 aagatttccc cgaacggcgg cacttgccgc cgttttgcat tctggggagt agcgcatgag
     1201 ccacgtgatg aacacctacg cccggcagcc ggtggccttc gtgcgcggcg aaggcgtctg
     1261 gctctgggac gaagcgggca agaagtacct cgatgccctg gcgggcatcg cggtgaatac
     1321 gctgggctac ggacatccca agctgacggc agccctttca aagagaattc agtccggggt
     1381 gctgcacacc tccaacctct ggcgcatccc ggaccaggaa aaggccgccg accgcatcgc
     1441 cgagatcacc ggcctggacg aggtgttctt ctgcaactcc gggctcgaag cgaacgaggc
     1501 ggcaatcaag gtcgcgcgca agtacggcca cgaccgcggc gtcgccgagc cggcgatcgt
     1561 ggtgatggag aaggccttcc acgggcgctc gctcgcgacc ctttcggcga ccggcagccg
     1621 caaggtgcag gcgggcttcg agccgctggt gtcgggcttc gtgcgcgtgc cgctcaacga
     1681 cctcgaggcg gtgcgtcagg tcgcggagca caacaagaac gtggtcgcgg tgttcgtcga
     1741 gccgatccag ggcgagggcg gcatcaacgt ctcgcgcctg gattacctgc gcggcctgaa
     1801 ggagatctgc gagcgcaagg agtggctgtt catgtccgac gaggtgcagt gcggcctcgg
     1861 gcgcaccggc aaatggttcg tctaccagca cgccggcttc ctgcccgacg tggttccgct
     1921 cgccaagggc ctcggctccg gcgtgcccgt gggcgcctgc gtcgtcggcg gccgcgccaa
     1981 gggcgtgttc aagccgggca accacggctc gaccttcggc ggcaacccgc tcgccatgac
     2041 cggcgtggtg gcgaccatcg acacgatcaa ggaagagggc ctgctcgcaa acgccacccg
     2101 cgtgggggac gtgatccgcg ccgggctttc ccagatccag ggaatttcgg aagtgcgcgg
     2161 catgggcctc atgatcggga tcgacgcggg cgtggcctgc ggcgagctgg tgcgccaggg
     2221 gctcgaggcg ggcctggtca tcaacgtcac cgccgacaac gtgatccggc tcctgccgcc
     2281 gctcgtcatg accgaagccg agggccgcct ggtggtcgag cgcctcgcgc cgctggtgcg
     2341 cgcgttcctc gagcgaagct cgcagcccaa agccgccgcg cactgaagtg gcgatggcgg
     2401 ttcgtcactt cctgcagttc cgggattttt cgctcgccga gctcgagcac gtgttcacgc
     2461 gcacgcgctg gatcaaggac aagttcaagc gctacgagcg ctactggccg ctcgaggacc
     2521 gcacgctcgc catgatcttc gagaagccct cgacgcgcac ccgcatgtcg ttcgaggcgg
     2581 gcatgcacca gctcggcggc gcggcgatct atctcttcac gcgcgactcg cagctcggac
     2641 gcggtgagcc ggtcgaggac gcggtgcagg tgatctcgcg catgtgcgac gtcatcatgc
     2701 tgcgcacttt cgagcaggac atcatccagc gcttcgccgg cagctcgcgg gtgccggtga
     2761 tcaacggcct caccaacgag tatcacccgt gccagatcct cgccgacgtg ttcacttacg
     2821 tcgagcaccg cggcgcgatc cgcggcaaga ccgtggcgtg gatcggcgac tccaacaacg
     2881 tctgcaatac ctggctgcag gcggcggcgc tgttcgattt caagctgcac gtctccacgc
     2941 cgcccggcta tgaaatcgag aaagagcgga tcaaccgcaa ctgcgaggtg ttcgacgagc
     3001 cgatgaaggc ggcgaaaggc gcggacctcg tcaccaccga cgtctggacc tcgatgggct
     3061 tcgaggccga gaacgaggag cgccggcgcg acttccagga ctggcaggtc gacgccgaga
     3121 tgatgcgcgc ggcgaagaag gacgcgctct tcatgcactg cctgccggcg caccgcggcg
     3181 aggaggtcgc cgccgaggtg atcgacggca agcagtcggt ggtctgggac gaggcggaaa
     3241 accggctcca cacccagaaa gcgttgctcg agttcctggt tctgggaaag aaataagatg
     3301 ttcggcgttg ctgactactg ggcgttcgta gtcgcggtga ttgtcttcct ggcgatcccc
     3361 ggcgtcggca acctggcgat cattacctcg accagcaagg gccgtatcgt cggcgggctg
     3421 gcagcgacgc tgggcgtgat cgtcggcgac caggtgctca tgtggctggc cgtcgccggc
     3481 gtggcggcgg tgctcaccgc aaacccggcg ctgttcggcg tggtgcagta cgccggtgcg
     3541 atctacctgg cctacctcgg tatccgcatg gtgctcgcca agccgggcga tgcgccggtg
     3601 ctggagatgc agccgcgccg cttcttccgg caggcgatgc tcatcacgct gctcaacccg
     3661 aaggcaatcg tgttctacat ggccttcttt ccgctgttcg tcgatcctgc gcggcaccag
     3721 ggactgccca ccttcggcgt gatggccctg accatagccg tgctcacgtt tctctactgc
     3781 gtgatcgtga tctccatcac ccacttcgcc gccgatcgca tgcgcgccag cccgcgcgtg
     3841 gccgtgtggc tcaaccggat cgcgggcacc atgctgctcg gcttcggcgt gaagctcgcc
     3901 gtcggaaagt agctaccgct tcttcggctt ttccggccct agaaggggaa caccggatcc
     3961 atcccccagc agccctgcct ctccgtagac gcgcagcttg tcgcgcgtat cggcgatgtc
     4021 gaggttgcgc atgctgagct ggccgatgcg gtcgcccggc gtgaagaaca cttcctcgcc
     4081 cttctccatg gtcaggcgct cgggcttgta ggtgaggttc gggctcttcg tgtcgaggat
     4141 cgagtagtcg ttgccgcggc gcagctccag ggtcacctcc ccggtcaccg ccttggccac
     4201 ccagcgctgc gcggtctcgc gcagcatcat cgcctgcggg tcgaaccagc gcccctggta
     4261 caggagccgg ccgaggcgcc ggccgttcgt gcggtactgc tcgatcgtgt cctcgttgtg
     4321 aatgccggtc accaggcgct cgtaggcgat gtgcagcagc gccatgcccg gcgcctcgta
     4381 gatgccgcgg ctcttcgcct cgatgatgcg gttctcgatc tggtcagaca tgcccaggcc
     4441 gtgccggcca ccgatcgcat tggcgtcttc catcatcttc acgcaatccg agaagcgctg
     4501 gccgttcagc gccaccggct ggccctgcgc gaattccacg ctgaccctct cggctttcac
     4561 cttcacgtcg tcgcgccaga aggcgacgcc catgatcggc tggacgatcc cgatgccctt
     4621 gtcgaggaac tcgaggtcct tggcctcgtg cgtggcgccg agaagattgg agtcggtgga
     4681 ataggccttc tcggcgctca tcttgtagtc gaagccggcg ctcgccatgt aggcggacat
     4741 ctcgcgccgt ccgcccagct cgtcgatgaa gcgctggtcg agccacggct tgtagatccg
     4801 gagatccggg ttggcgagca gaccgtagcg gtagaaacgc tcgatatcgt tgcccttgta
     4861 cgtgctgccg tcgctccaga cgccgacgcc gtcctcgcgc atcgccacga ccagcatcgt
     4921 gccggtgatc gcgcggccga gcggcgtggt gttgaagtag gtggcaccgc cggtcgagat
     4981 gtggaaggcg ccgcattgca gggcggcgat gccttcggcg acgagctgcg gacgacactc
     5041 gaccagtcgc gccttctgcg ccccgtagct cagcgccttg cgcggaatgt ccgcgtagtc
     5101 ggactcgtcg ggctggccga ggtgtgcggt atacgcgtag gggatcgcgc ccttcgagcg
     5161 catccagtgc accgccgcgc tcgtgtcgag gccgcccgag aaggcaatgc cgattttctc
     5221 gcccttcggc agcgtgccga gaatcgtccc caagtcagtc tccggaaaac tgcatgatac
     5281 cgctcgggct agaatggccg cagtgcgcgc ccttgcctcc gtccttgccg cccttctcgc
     5341 ggcctcgtcg cagccgtgtg ctgccgcacc tttcgccgtg caggtcggcg aagcgcgcat
     5401 cgggctcgac gccccgcccg gcttcgcgga cacgaccgcc accggctcgc cgcggctgca
     5461 ggagctgggc gaggccacga cctccgcgtc gaaccgcgtg ctgctgttcg cggtctccga
     5521 cgccgacctg cgccgcttca cgctcggcga cccgctcgag ctcaggcgct acatggtggt
     5581 cgtgacgccg cgcgccatgg agcgcgagcg catgtccgag agcgcgttcc aggcgttcgc
     5641 cggcggctcg ctgcgcgagc tgggaccggt gtccggcgcc acggactacc agaagtacct
     5701 cgactcgcgg cccgtcgggg cggtcagcac gctcgccgag ctgcgcaagg atccggacgc
     5761 ggtcgcggtg ctgcagggct cgcgcagcaa gggcgggttc ctggagcgct cgaaattcgt
     5821 gctgtcttcc accagcgtcg tcctgctgcg cggcaaggcg ctcaccctct ctatctttac
     5881 gcagtacgac gatcccgccg atctcgaatg gatccgcaac gccaccgcgc gctggatcga
     5941 cgagctgaaa cggctcaact cgcgctagag cgacggcttc cgatgccttc cttcgacatc
     6001 gtttcccagg tcgaccagca ggaagtgaag aacgcggtcg agcagaccaa caaggaagtc
     6061 accaaccgct tcgacttcaa gggctcggac gcccgcgtgg agcagagcga gctggtcctg
     6121 accgtgtacg ccgacgacga gttcaagctc ggccaggtgc tcgacgtgct gcgcacgcgc
     6181 ctcgcgaagc gcggcgtcga cgtccggtcg ctcgagcccg gcgcagtgga gaagatctcg
     6241 ggcgacaagg tgaagcggcc ggtcacggtc aaggtgggcg tgccgcagga gaagggcaag
     6301 cagatccaga agctcgtcaa ggactcgaag ctcaaggtcg ccgcctcgat ccagggcgac
     6361 gcggtgcgaa tttcaggcgc gaagaaggac gagctgcagt cggcgattca gctcgtgcgc
     6421 aagtcggtag cggacttacc cctgcaattc atcaacttcc gggattgaag cgggggaaaa
     6481 cctcccccgc ttacccccta ctgaggcctt cgctccaccg gtcgtagatc ttgtccgcga
     6541 cctcgtgcag ccggtcgagg cgcggcgcga ggtccttctc gatctccgcc ggcggctgca
     6601 ccgccgggct tttcttgtgc tcgcggattt cctcgctccc ggtggaaagc caggtgcgca
     6661 cgagctccgg ggtcatctcg acgtggcatt gcatggcgaa gtgccgcccg agcgcgaagg
     6721 cctggttggc gcagtgctcg ccctcgagga cgcgcgtggc gccgggcggg atgctgaagg
     6781 tctcgccgtg ccagtggaag gactcgaacg cgggcgcgcc gaaccacgcg cgcgccacgc
     6841 cgttgtcggc gacctcgacc tcgccccagc cgatctcctt cacgcgcgcg cgcgtgactt
     6901 cgccgcccag cgccttcgac atcagctggc cgccgaggca atgcccgagc gcgggcacgt
     6961 ccttgcgcac cgcctcgcgc accagttcga gcagcggccg gatccagggc aggtcgtcgt
     7021 tgacgctcat cggcccgccc atg
//
LOCUS       DATBSZ010000276         2682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_874970, whole genome shotgun sequence.
ACCESSION   DATBSZ010000276 DATBSZ010000000
VERSION     DATBSZ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874970"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..768)
                     /locus_tag="VIG70_18035"
     CDS             complement(64..768)
                     /locus_tag="VIG70_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8252497.1"
                     /translation="MPGLLEGDRALVTGAGRGIGRAIADAVEAEGATVLRADLADADL
                     SSPEGAAKLGEAAIRKLGSVSIFVHCASPPRQEAKHMLAVNFAEWREMLSVNLDAAFV
                     LGQKLGLHMKEKKIRGRMLISTSLHAESPRNLPHYSAAKAGQTMLVKELARALGPHGI
                     RVNAIVPGAIPGEGFKGLPGMEKTIPLGRFGTPAEVAAMALAVLSERFGAYVTGASIL
                     VDGGIALHNWIPPSNT"
     gene            833..1399
                     /locus_tag="VIG70_18040"
     CDS             833..1399
                     /locus_tag="VIG70_18040"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8252498.1"
                     /translation="MATLHRERVLRAATTSFLARGYGSSVDDIARRAGVAKQTVYQHF
                     ANKDELFKAVAGDLAKRVLVKLEGAGNQNEVRQSLLRFALEYRKRALGAQGIATFRTL
                     VPEVPRFRALARTMYANTAGEMVRRLAEFLRQKLDAPDPEFAAEMFLSMLAGHDRLKR
                     LFAVPPGAESEAARTARIVDLFLRVHQP"
     gene            1396..2493
                     /locus_tag="VIG70_18045"
     CDS             1396..2493
                     /locus_tag="VIG70_18045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8252499.1"
                     /translation="MRLLIVFLCMAVAACSDSGAKQQEKKAAKDAAPAAVPVTTLEMA
                     PRAVPISYEAVARTEGSREVQVRARVTGILEHQLYTEGDTVKAGQLLFRIERAPFEIE
                     LQQARGVLAQEVARRDLARQEAERLKSLVDRRAISQKEADQAASSLQQTGAAVQIAQA
                     RVRQAELNLSYTEVRAPIDGITGRAMQSVGSLVSPNAESALLTTLTLGDPIWVRFSIS
                     EAEFSRVRGSETIEVALELPDGKQYPHTGKLNFAGSTVDAATGTVQMRAEVPNPELAL
                     LPGQFVRVHVIAGTQQAIVVPQTAVLQNEGGRYVWLAAEGKAQQRPIRAGNWIGPDWV
                     VLDGLKAGDQVIVDNLGRLRPGTPVQARKAG"
     gene            2494..>2682
                     /locus_tag="VIG70_18050"
     CDS             2494..>2682
                     /locus_tag="VIG70_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8252500.1"
                     /translation="MSRFFIHRPVFAGVIAIIIVLAGLVSAKLLAVAQYPEIAPPTVR
                     IDTTYPGASAETLARTVAA"
BASE COUNT          417 a          918 c          941 g          406 t
ORIGIN      
        1 ggcggaaatt ccgcgtaccg gtgtgattgg ccggcgagta cggctttacg tcggattccc
       61 ggatcaggta tttgatggcg gtatccagtt gtggagcgcg atgccgccgt cgaccaggat
      121 cgacgccccg gtcacgtagg cgccgaagcg ctcggagagc accgcgagcg ccatcgccgc
      181 gacctcggcc ggggtgccga agcggccgag cgggatggtc ttttccatgc cgggcagccc
      241 cttgaatccc tccccgggga tggcgcccgg gacgatcgca ttgacgcgga ttccgtgcgg
      301 ccccagcgcc cgcgcgagct ccttcaccag catcgtctgc ccggcctttg cggcgctgta
      361 gtgcggcagg ttgcgcggcg actcggcgtg cagggaagtg ctgatcagca tgcgccccct
      421 gatcttcttc tccttcatgt gcaggccgag cttctggccg aggacgaagg ccgcatcgag
      481 gttgaccgag agcatctcgc gccactcggc gaagttgacc gctaacatgt gcttcgcttc
      541 ctgccgcggc gggctcgcgc agtgcacaaa gatcgagacg gatccaagct tgcggatcgc
      601 cgcttcgccc agtttcgcgg cgccctcggg gctcgacagg tcggcgtcgg cgaggtcggc
      661 gcgcagcacc gtggcgccct cggcttcgac ggcatcggcg atggcgcggc cgatgccgcg
      721 cccggcgccg gtcacgagcg cgcgatcccc ctccagcaag ccaggcatga gcccagtatc
      781 ccacagcttc tgctagactg accagtctag ttcaggggac tccgcagagc cgatggccac
      841 gcttcaccgc gagcgcgtac tgcgcgccgc caccacctct ttcctcgcgc gcggctatgg
      901 ctcgagcgtc gacgacatcg cgcgccgcgc cggcgtcgcc aagcagaccg tgtatcagca
      961 cttcgcgaac aaggacgagt tgttcaaggc ggtggccggc gatctggcga agcgcgtgct
     1021 ggtcaagctc gaaggcgcag gaaaccaaaa tgaggtaagg cagagcctgt tgcgctttgc
     1081 actcgagtac cgcaagcgcg cgctgggcgc gcagggcatc gctaccttcc gcaccctggt
     1141 gcccgaggtg ccgcggttcc gcgcgctcgc ccgcaccatg tacgcgaata cggccggcga
     1201 gatggtgcgg cggctggccg agttcctgag gcagaagctc gacgcgccgg accccgagtt
     1261 cgcggcggag atgttcctct ccatgctcgc gggccatgac cgcctgaagc gcctgttcgc
     1321 ggtgccgccg ggcgccgaga gcgaagccgc gcgcaccgcg cgcatcgtcg atctttttct
     1381 gagggttcat cagccatgag gctcctgatc gttttcctgt gcatggcggt cgccgcctgc
     1441 agcgacagcg gggcgaagca gcaggagaag aaagcggcca aggacgcggc gccggccgcg
     1501 gtgccggtca ccacgctcga gatggcgccg cgcgccgtgc cgatcagcta cgaggcggtg
     1561 gcgcgcaccg agggctcgcg cgaagtgcag gtgcgcgcgc gcgtcaccgg gatcctcgag
     1621 caccagctct acaccgaggg cgacacggtc aaggccggcc agctgctgtt ccgcatcgag
     1681 cgagcgccct tcgagatcga gctgcagcag gcgcgcgggg tgctcgcgca ggaggtcgcg
     1741 cggcgcgacc tggcgcggca ggaagccgag cggctgaaga gcctggtcga ccgccgcgcc
     1801 atcagccaga aggaagccga ccaggcggcg tcctcgctgc agcagaccgg cgccgcggtg
     1861 cagatcgcgc aggcgcgcgt gcgccaggcg gagctcaacc tctcctatac cgaggtgcgg
     1921 gcgccgatcg acggcatcac cgggcgcgcc atgcagtcgg tcggcagcct ggtgtcgccg
     1981 aacgccgagt ctgcgctgct caccacgctc accctgggcg atccgatctg ggtgcgcttc
     2041 tcgatctcgg aagccgagtt ttcccgcgtc cggggaagcg agacgatcga ggtcgcgttg
     2101 gagctgccgg acggcaagca atacccgcac acgggaaagc tcaacttcgc cggctcgacc
     2161 gtcgacgccg ccaccggcac ggtgcagatg cgcgcggagg tgccgaaccc cgagctcgcc
     2221 ttgttgcccg gccagttcgt gcgcgtgcat gtgatcgcgg gcacgcagca ggcgatcgtg
     2281 gtgccgcaga ccgcggtgct gcagaacgag ggcgggcgct atgtctggct ggcggcggag
     2341 ggcaaggcgc aacagcggcc gatacgcgcc ggcaactgga tcggccccga ctgggtggtg
     2401 ctggacggcc tgaaggccgg cgaccaggtg atcgtcgata acctcggccg cctgcgtcct
     2461 ggcacgccgg tgcaggccag aaaagcaggc tagatgtcgc ggttcttcat ccaccggccg
     2521 gtttttgccg gcgtcatcgc catcatcatc gtgctggcgg gcctcgtctc ggcgaagctg
     2581 ctcgcggtcg cccagtaccc cgagatcgcg ccgccgacgg tgcgcatcga caccacctat
     2641 cccggcgcca gcgccgagac gctcgcgcgc accgtggccg cg
//
LOCUS       DATBSZ010000277         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1216543, whole genome shotgun sequence.
ACCESSION   DATBSZ010000277 DATBSZ010000000
VERSION     DATBSZ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216543"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VIG70_18055"
     CDS             <1..350
                     /locus_tag="VIG70_18055"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8252501.1"
                     /translation="AHVARSGGHAELGVSVLPGHRGRGIGGALLARAHLRARNWGVWA
                     LFMHCLTENEAMMHLARKQAMEIVVESGEADAWLRLAPADASSHFGEVFAQRVALFDY
                     ALKQGRSWLGQGR"
     gene            complement(313..1686)
                     /gene="mgtE"
                     /locus_tag="VIG70_18060"
     CDS             complement(313..1686)
                     /gene="mgtE"
                     /locus_tag="VIG70_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY8252502.1"
                     /translation="MNAQAPARLEVEDLQQNLLEVQELLQEGKPKPGLRAKLDPLHAA
                     DIAYILEALPLEERLAVWELVKADRDGEILVEVSDAVRESLIEAMDSRELVAAAETLE
                     ADELADIAPDLPASVIEEVVQALPAEERERLRAALSFPEGAVGALMDFDHIAVRADTT
                     LEAATRYLRRLDELPDHTDQLFVVDRDQVLKGTLPLARLIVSDLEREVHQVMVAESVK
                     LYPQDRAEDAASAFERYDLISAPVVDASGRLVGRLTVNAVVDYIRERSAESQLAEAGL
                     QHEEDVFAPVLDSFKNRWAWLAINLVTAFIASRVIGVFEDSIAQLVALAALMPIVAGI
                     GGNSGNQTTTMIVRALALGQIHGGYWRKLLAKEVGVALLNGAVWGTLLGAIAYLFYGN
                     VALGGVMALAMILNLLLAAMMGVTIPWLRARFGRDPAVGSSVLITACTDSGGFFIFLG
                     LATIFLV"
     gene            complement(1697..2401)
                     /gene="mtgA"
                     /locus_tag="VIG70_18065"
     CDS             complement(1697..2401)
                     /gene="mtgA"
                     /locus_tag="VIG70_18065"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEY8252503.1"
                     /translation="MARKRFRVLVLAWKAFCYTLGTVLLAVIAIQLWFFSHIVYWSSF
                     NPSSTAFMDQHMEKPGARLRHQWVPYSRVSEHLKRAVVAAEDAKFLDHEGFDWEAIQK
                     AIQKNEQRGKVVAGASTITQQLAKNMFLSGYRSWLRKGQEAVITWMMERTLSKRRILE
                     IYLNVAEWGEGIFGAEAAARYHFGVSAAALGPEQAAWLAAILPSPKRYERGRRTPYLE
                     GRMVTISSRMTGAQIP"
     gene            complement(2391..3185)
                     /gene="aroE"
                     /locus_tag="VIG70_18070"
     CDS             complement(2391..3185)
                     /gene="aroE"
                     /locus_tag="VIG70_18070"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY8252504.1"
                     /translation="MSDRYAVIGNPVAHSKSPWIHAEFARATGQDIDYGRIEAPLDGF
                     AQAVERFRAAGGKGLNVTVPFKQQAFALSRDASERARIAKAGNTLVLDRSFCDNTDGV
                     GLVRDITVNLGLTLKGKRLLLLGAGGAAQSVVGALLEAGAVQLSIANRTASRAVELAA
                     RFPGASGCGYEGLSGKSFDVVINATSAGLSDAAPPLPKGLLNDSTLAYEMVYGRETPF
                     LAAAHKAGAHTCDGLGMLVEQAAESFFLWRGVRPATSPVLAKLRGS"
BASE COUNT          536 a         1132 c         1066 g          524 t
ORIGIN      
        1 cagcgcacgt cgcccgcagc ggcgggcacg ccgagctcgg ggtctctgtg ctgccggggc
       61 accgcggccg cggcatcggc ggtgcactgc tcgcgcgcgc gcacctgcgc gcccgcaatt
      121 ggggcgtgtg ggcgctcttc atgcactgcc tgacggagaa cgaggccatg atgcacctcg
      181 cgcgcaagca ggcgatggag atcgtggtcg aatcgggcga agccgatgcc tggctcaggc
      241 ttgcgcccgc cgacgcctcg agccatttcg gcgaggtgtt cgcccagcgc gtggcgctct
      301 tcgactacgc gctcaaacaa ggaagatcgt ggctaggcca aggaagatga agaacccgcc
      361 cgaatcggtg caggcggtga tcagcaccga ggagccgacc gccgggtcgc gcccgaagcg
      421 cgcgcgcagc cacgggatcg tcacgcccat catggcggcg agcagcagat tgaggatcat
      481 ggcgagcgcc atcacgccgc cgagcgcgac gttgccgtag aagagataag cgatcgcgcc
      541 gagcagcgtt ccccagaccg cgccgttcag cagcgccacg ccgacttcct tcgcgagcag
      601 ctttctccag tacccgccgt ggatctgccc gagcgccagt gcgcgcacga tcatggtggt
      661 ggtctggttg cccgagttgc cgccgattcc ggccacgatc ggcatcagcg cggcgagcgc
      721 cacgagctgc gcgatcgagt cctcgaatac gccgatgacg cgcgaggcga tgaacgcggt
      781 gacgaggttg atggcgagcc aggcccagcg gttcttgaag ctgtcgagca ccggcgcgaa
      841 cacgtcttcc tcgtgctgca ggccggcttc ggcgagctgg gactcggcgc tcctttcgcg
      901 gatgtaatcg accaccgcat tgaccgtgag ccgcccgacc agcctgccgc tcgcatccac
      961 caccggcgcc gagatgaggt cgtagcgctc gaaggcgctc gccgcgtcct cggcgcggtc
     1021 ctgggggtag agcttgacgc tttccgccac cattacctgg tgcacctcgc gctcgaggtc
     1081 cgaaacaatg aggcgcgcga gcggcagcgt gcccttcagc acctggtcgc ggtccactac
     1141 gaagagctgg tcggtatggt cgggtagctc gtcgagacgc cgcagatagc gcgtcgccgc
     1201 ctccagcgtg gtgtctgcgc gcaccgcgat gtgatcgaaa tccatcagcg ccccgaccgc
     1261 gccctcgggg aaagagagcg cagcgcgcag ccgctcgcgc tcctcggcag gcagcgcctg
     1321 caccacctcc tcgatgacgc tcgccgggag gtccggggca atgtccgcga gctcgtcggc
     1381 ctccagcgtc tcggccgcag ccaccagctc gcgcgagtcc atcgcttcga tgagcgattc
     1441 gcgcaccgcg tcggagactt cgaccaggat ctcgccgtcg cggtcggcct tgaccagctc
     1501 ccagaccgcg agccgctcct cgagcggcag cgcctcgagg atgtaggcga tgtcggcggc
     1561 gtgcagcggg tcgagcttgg cgcgcagccc gggcttcggc ttgccctcct gcaacagctc
     1621 ctgcacctcc agcaggttct gctgcaggtc ctcgacctcg agccgcgccg gagcctgcgc
     1681 gttcacgaga aatgagtcat ggtatttgtg ccccggtcat gcggctcgaa atggtcacca
     1741 tgcgcccctc cagatagggc gtgcgccgcc cgcgctcgta gcgcttcggg ctgggcagga
     1801 tggccgcgag ccacgcggcc tgctcgggcc cgagcgccgc cgccgacacg ccgaagtgat
     1861 accgcgccgc cgcctcggcg ccgaagatgc cctcgcccca ttccgccacg ttcagataga
     1921 tctcgaggat gcggcgcttg gagagcgtgc gttccatcat ccaggtgatc accgcctcct
     1981 gccccttgcg cagccacgag cggtagcctg agaggaacat gttcttcgcg agctgctggg
     2041 tgatggtcga ggcgccggcg accaccttcc cgcgctgctc gttcttctgg atcgctttct
     2101 ggatcgcctc ccagtcgaac ccctcgtggt cgagaaactt ggcgtcctcg gcggccacta
     2161 ccgcgcgctt gagatgctcc gagacgcggc tatagggaac ccactggtgt cgcagccgcg
     2221 cgcccggctt ttccatgtgc tggtccatga aagcggtgga cgacgggttg aagctcgacc
     2281 agtagacgat atgcgagaaa aaccagagct ggatcgcgat cacggcgagc agcaccgtgc
     2341 cgagcgtgta gcagaacgcc ttccacgcga ggaccaggac gcgaaagcgc ttacgagcca
     2401 cgcagcttcg cgagcaccgg gctcgtcgcc gggcgtaccc cgcgccaaag gaagaacgat
     2461 tcggctgcct gctccaccag catgccgagg ccgtcgcagg tgtgcgcgcc cgccttgtgc
     2521 gcggccgcca gaaacggcgt ctcgcggccg tacaccatct cgtacgcaag ggtggaatcg
     2581 ttcaacagcc ctttgggcaa gggcggtgcg gcatcgctca atccagccga ggtcgcgttg
     2641 atgaccacgt cgaacgactt gcccgacaac ccttcatagc cgcatcccga agcccctgga
     2701 aaacgcgcgg cgagctccac tgccctgctc gccgtgcggt tcgcaatcga gagctggacc
     2761 gcccccgcct cgagcagcgc gccgactacg ctctgcgcag ctccgcccgc acccagtaga
     2821 agcaagcgct tacctttcag ggtgagcccg aggttcaccg tgatgtcccg gaccagccct
     2881 actccgtcgg tgttgtcgca gaaggaacgg tccagaacca gggtattgcc ggcctttgct
     2941 atgcgcgctc gctcgctcgc gtccctgctg agcgcaaacg cctgctgctt gaacggcacg
     3001 gtgacgttaa ggcccttgcc gccggcggcg cgaaagcgtt cgaccgcctg cgcgaagccg
     3061 tcgagcggcg cctcgatgcg gccgtagtcg atgtcctgcc ccgtggcgcg cgcgaactcc
     3121 gcgtggatcc agggggactt ggaatgcgcg accgggttgc cgatgaccgc gtaccggtcg
     3181 ctcatctcgc tactcagtcc agatcttgtc gccctggccg aagaaccaag ttcgcgtaat
     3241 gaccaagaga tcggtgtc
//
LOCUS       DATBSZ010000278         7018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_260770, whole genome shotgun sequence.
ACCESSION   DATBSZ010000278 DATBSZ010000000
VERSION     DATBSZ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260770"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VIG70_18075"
     CDS             <1..523
                     /locus_tag="VIG70_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEY8252505.1"
                     /translation="PPPIRHVIKMLVPRVDQDGNEMGGVPTVLNDAPLGTYLGWNITA
                     QPTLTDPRAFHAGQVCNYVGGMVPFAKTKAQRLATGDPRLSLEERYGTHAGYVEAVKK
                     AANNAACKGYLLVGPEAATDGSMPPQKSWHCRAVTTAPAGFPNDWKVLVDQAIASNVC
                     NQPTDGGKCNPAP"
     gene            678..1982
                     /locus_tag="VIG70_18080"
     CDS             678..1982
                     /locus_tag="VIG70_18080"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEY8252506.1"
                     /translation="METGALKALARRSILCAAAAAFACAAAPGFATDQHGKRVVIPKS
                     GERCANDPKCHNRWHYAIPRVASADPGDVVVFGTRDAFDHSLDRNSTAADVVALNLNL
                     VHPLTGPLHVRGAKAGDVLAVTLLDIEPDDFGYTIVVPGFGFLRDVFTEAFIVRWDLT
                     RRGATSRDMPGVKIRFDGFMGTIGVAPGQKETEKFFQREAELAAAGGVVLTPQPLDAL
                     PAPVCGVAGTHRDRCLRTIPPRENGGNMDVKQMQVGTTLLLPCFVDGCLLSIGDVHFA
                     QGDGEVSGTAIEMDATVTVKVEVRKGQGARITQPHYEGGDQLKRLAPSKFYATVGYPI
                     KPKGLVPPTHAYLDPSGTRIGPLSNLSEDVTLAARDALLQMVDWIHRNKKLTREQAYV
                     LSSVAVDLRIGNLVDVPNFAVSAILDLGVFDDRRDRDDDDRR"
     gene            1993..2544
                     /locus_tag="VIG70_18085"
     CDS             1993..2544
                     /locus_tag="VIG70_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252507.1"
                     /translation="MNRRRLLRVTAAAGAWLLLTAHSPYRQWEAHRKMRLVVLVSAAE
                     QPSVALGSALALIYTQRLPESRAIMARARDTNDAVRLIASKQLDVALLRTADAYAAAA
                     GEAPYADSGAPRLRALALLGDYVFVCRDDLPVAAAYMLTEALAEHWRDIDPPLVGAAP
                     GPRPPNTLAVPLHPGALEYYRDH"
     gene            complement(2541..4076)
                     /locus_tag="VIG70_18090"
     CDS             complement(2541..4076)
                     /locus_tag="VIG70_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252508.1"
                     /translation="MTSQAATQVRWWVPGDWNGFFGLFTNVLLNVIVLTGLCLGVVQL
                     PADTVFGRILPALGIALPLGNLYYAYLAYRLAQKEGRSDVTAMPYGPSVPHMFIVVFV
                     IMLPVYLKTKDPVLAWQAGLAWAFIIGVIVLAGIFVGPWIRAHTPRAAMLGTLAGISI
                     AFISLRPAFQMWEVPWIAFISLAIVLISWTANTRLPFGLPGGLAAVIVGIVVGWIAYF
                     AGWSDVVKAEQVRASLGQFGLHFPLLGTEVWRGLADVAPLLVTAIPLGIYNFTEAMNN
                     VESASAAGDNYDLRKILAADGIGAIVGAMLGSPFPPAVYIGHPGWKAVGGRIGYSLAT
                     GVLMGLVCFLGLTALLLAVVPLVAILPILLYIGLVIGAQAFQASPQSHAPAVVLAIIP
                     NVAAWGQLQVDSALNAAGTSAAQVGMAKLGGSGLVYHGMELLGGGAVLAGLVLGAVAA
                     LIIDRRLKEAALWSFAGAVLAFFGFIHGTQLGWAMSPQVALGYALFGAVCLAASMQTE
                     KAN"
     gene            complement(4073..4744)
                     /locus_tag="VIG70_18095"
     CDS             complement(4073..4744)
                     /locus_tag="VIG70_18095"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY8252509.1"
                     /translation="MARVQAEPYEFEFDARACALVIIDMQRDFVDPGGFGEALGNDVS
                     LLRKAIAPTKKVLEAARNRKLLVIHTREGHRADLSDLPQSKKLRGRLKVGIGDPGPMG
                     RILVRGEYGHDIVDDLKPLPGEPVVDKPGKGAFYATDLDSMLRNRGIRSLVVCGVTTE
                     VCVNTTVREANDRGYDCLVLEDCVGSYFPEFQKAALDMIKAQGGIFGWVSDSKKFLEG
                     LKGRP"
     gene            4865..>7018
                     /locus_tag="VIG70_18100"
     CDS             4865..>7018
                     /locus_tag="VIG70_18100"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEY8252510.1"
                     /translation="MKPAVRAAFAVLLPVALAGIGEPAEARITKIEITSRAIAFGGAS
                     FGSVGQYETLRGVAYGEVDPNDPLNEVITDIKLAPRNSRGKVEYNMDVWINKPLDMTR
                     ANGTLLHDVPNRGNVRSLEINVGGAADLNAGDGWLQRQGYVMSDNGWQGDVSTGLQIR
                     LPIARNKNGTPIVNRIRAEYIQGSPTSTVDITTPPSYEAVSTNNAGATLTRRVHQDDP
                     KELIPNADWAFADCSTVPFPGVPSTTKVCLKGNFDTNHIYELLYDAKNPTVLGLGFAA
                     TRDFVSFLRNSNGAGSDCRDDDHGHHGHHGKRHDDDDDDDDDGGDRRHGPCRVKVQMP
                     ENPLGRSIQNAIIYGSSQSGRYIRTFIQLGFNRDENHNMVLEGAIPHKASNRGAFNVR
                     FGQPDRLSGTQHTERQFPGGESSQTWGEYHDRISGVKAGQLDRCRKTDQCPKIFHTNT
                     DTEYWQALMALNTTNSEGTEDLKIPSNVRIYLFAGTRHGGGNVLAQPPATVPAPPAGC
                     QMPNNSNPFIHGQRALLVALRAWIVDGVRPPSSLYPKLRDGTLARLRDIDYPYLPAVN
                     FTLAGVTTQKFYLDRGPLFNVEDISGVMREPPIKRGAYPVRQPQVDQDGNTIAGLPST
                     AMLVPLGTYLGWNVRRAGFSEGDSCDLNGAYVPFFRTLAQRQAAGDPRPSLQERYPTH
                     ADYVAKVTAAANSLVARRLLLPEDAAFLINAANAAA"
BASE COUNT         1230 a         2449 c         2331 g         1008 t
ORIGIN      
        1 gccgccgccg atccggcacg tcatcaagat gctggtgccg cgcgtcgacc aggacggcaa
       61 cgagatgggc ggcgtgccga ccgtgctgaa cgacgcaccg ctcggaacct acctcggctg
      121 gaacatcact gcccagccta ccctgaccga cccgcgggca tttcacgcgg gccaggtgtg
      181 caactacgtc ggcggcatgg tgcctttcgc caagacaaag gcgcagcgcc tggcgaccgg
      241 cgacccgcgc ctgtcgctcg aagagcgcta tggcacgcac gccggctacg tcgaggcggt
      301 gaagaaggcc gccaacaacg cggcttgcaa gggctacctg ctcgtaggtc cggaagcggc
      361 caccgacggc tcgatgccgc cgcagaagtc gtggcactgc cgcgccgtca cgaccgctcc
      421 ggcaggcttc cccaacgact ggaaggtgct ggtcgaccag gcgatcgcga gcaacgtgtg
      481 caaccagccg accgacggcg gaaagtgcaa cccagcaccc tgaggaagca gtagaggaaa
      541 gcgtgaggcg gcgaattgcc gcctcacgcc agcttcacat ggaatgattt ccattaattg
      601 aggatgggtc gcaattgatg tgcgcttctc atgaacgcct cgcccggcag gcgtagcatt
      661 ttcgaaggag gtaaaccatg gaaactggag cgttgaaggc gctcgcgcgc cgttcgatcc
      721 tgtgcgccgc cgcggcggcg ttcgcctgcg cggctgcccc cggctttgcg acggaccagc
      781 acggcaagcg cgtagtcatt ccgaaaagcg gcgagcgctg cgcgaacgac ccgaagtgcc
      841 acaaccgctg gcactacgcg ataccgcggg tggcgtcggc cgacccgggc gacgtagtgg
      901 tgttcggaac gcgcgacgcg ttcgaccact cgctcgaccg taattcgacc gcggccgatg
      961 tggtggcgct caatctcaac ctcgtgcatc cgctcaccgg gccgctccac gtgcgcggcg
     1021 caaaggcggg cgacgtgctg gccgtgacgc tgctcgacat cgagcccgac gacttcggct
     1081 acaccatcgt cgtgccgggc ttcgggttcc tgcgcgacgt gttcaccgag gccttcatcg
     1141 tgcgctggga cctcacgcgc cgcggcgcga cgtcgcgcga catgccgggg gtgaagatcc
     1201 gcttcgacgg attcatggga acgatcggcg tcgccccggg ccagaaagag accgagaagt
     1261 tcttccagcg tgaagccgag ctcgccgctg caggcggcgt ggttcttact ccccagccgc
     1321 tcgatgcgct gcccgcgccg gtctgcggcg tcgccggcac gcaccgggac cggtgcctgc
     1381 gcacgatacc gccgcgcgag aacggcggga acatggacgt gaagcagatg caggtcggca
     1441 cgacgctgct ccttccctgc ttcgtcgacg ggtgcctgct ctccatcggc gacgtgcatt
     1501 tcgcgcaagg cgacggcgag gtctcgggca ccgcgatcga gatggacgcg accgtgaccg
     1561 tcaaggtcga ggtgcgcaag ggacaaggcg cgcgcatcac ccagccgcat tacgagggcg
     1621 gcgaccagct caagcgcctc gcgccgagca agttctacgc gactgtcggc tacccgatca
     1681 agccgaaggg cctggtgccg ccgacgcatg cgtatctcga cccgagcggt acgaggatcg
     1741 gcccgctttc caacctttcc gaggacgtga cgctggcggc gcgcgatgcg ctgctgcaga
     1801 tggtcgactg gatccaccgg aacaagaagc tgacgcgcga gcaggcgtac gtgctctcga
     1861 gcgtcgccgt cgacctgcgg atcggaaatc tggtcgacgt gcccaatttc gccgtgtcgg
     1921 cgattctcga cctcggggtg ttcgacgatc ggcgcgaccg ggacgacgac gaccgccggt
     1981 agggggcggc cgatgaatcg acgccggctg cttcgcgtca cggcggcagc cggcgcctgg
     2041 ctgttgctca ccgcgcattc gccttaccgc cagtgggagg cccatcgcaa gatgcgcctc
     2101 gtcgtgctgg tgagcgcagc cgagcagccg tcggtggctt tgggcagcgc gctcgcgttg
     2161 atctacacgc agcggctgcc ggagagccgc gccatcatgg ctcgcgcgcg cgacaccaac
     2221 gacgccgtgc ggttgatcgc gagcaagcaa ctcgacgttg cgctgctgcg caccgccgac
     2281 gcctacgccg cggctgccgg cgaagctccg tacgccgaca gcggcgcacc tcggctgcgc
     2341 gcgctcgccc tgctgggcga ctacgtgttc gtgtgccgcg acgaccttcc ggtcgcagcc
     2401 gcctacatgc tcaccgaagc gctcgccgag cattggcgcg acatcgatcc cccgctagtg
     2461 ggcgcggcac ccggcccgcg tcccccgaac acgctggcgg tcccgctcca ccccggcgcg
     2521 ctcgagtact accgggatca ctagttcgcc ttctcggtct gcatgctggc ggcgaggcac
     2581 accgcgccga acagcgcgta gccgagcgcg acctggggcg acatcgccca gccgagctgc
     2641 gtgccgtgga tgaagccgaa gaacgcgagc accgcgccgg cgaaggacca tagcgccgct
     2701 tccttgagcc ggcgatcgat gatcagggcg gcgaccgcgc cgagcaccag cccggcgagc
     2761 accgcgccgc cgccgagcag ctccatgccg tggtacacga ggcccgagcc gccgagcttg
     2821 gccatgccca cctgcgccgc cgaggtgccc gccgcgttga gcgcgctgtc gacctggagc
     2881 tggccccagg cggcgacgtt cggaatgatg gcgagcacca ccgcgggcgc gtggctctgc
     2941 ggcgaggcct ggaaggcctg cgcgccgatc accaggccga tgtagagcag gatcggcagg
     3001 atcgcgacca gcggcaccac cgcgagaagc agcgccgtca ggccgaggaa gcacaccagc
     3061 cccatcagca ccccggtggc gagcgagtag ccgatgcgcc cgccgaccgc cttccagccc
     3121 gggtggccga tgtacaccgc gggcgggaac gggctgccga gcatcgcgcc cacgatggcg
     3181 ccgatgccgt cggcggcgag aatcttgcgc aggtcgtagt tgtcgcccgc ggccgaagcg
     3241 ctctcgacgt tgttcatcgc ctcggtgaag ttgtagatgc cgagcgggat cgcggtcacc
     3301 agcagcggcg ccacgtcggc gaggccgcgc cacacctcgg tgccgagcag ggggaagtgc
     3361 aggccgaact gcccgagcga ggctcgcact tgctcggcct tgaccacgtc gctccagccg
     3421 gcgaagtagg cgatccagcc caccacgatg ccgacgatca ccgcggcgag gccgcccggc
     3481 aggccgaacg gcaggcgcgt attcgcggtc cagctgatca gcacgatcgc gagcgagatg
     3541 aacgcgatcc acggcacctc ccacatctgg aacgccgggc gcagcgagat gaaggcgatc
     3601 gagatgccgg cgagcgtgcc gagcatcgcc gcgcgcgggg tgtgcgcccg gatccacggc
     3661 ccgacgaaga tcccggcgag cacgatgacg ccgatgatga aggcccaggc gaggcctgcc
     3721 tgccacgcga gcaccgggtc cttggtcttc aggtacaccg gcagcatgat cacgaacacc
     3781 acgatgaaca tgtgcggcac gctcgggccg tagggcatcg cggtcacgtc gctgcggcct
     3841 tccttctgcg ccagccggta ggcgaggtac gcgtagtaga ggttgccgag cggcagcgcg
     3901 attccgagcg ccggcaggat gcgcccgaac acggtatcgg cggggagctg aaccaccccc
     3961 aggcacaggc ccgtgagcac gatcacgttg agcagcacat tcgtgaaaag gccgaagaat
     4021 ccgttccagt ccccgggcac ccaccatcgg acttgcgttg ccgcttggct tgtcatggtc
     4081 tccctttcaa tccttctaaa aatttcttcg aatcgctaac ccagccgaaa atccccccct
     4141 gggccttgat catgtcgagc gccgccttct ggaactcggg aaagtacgag ccgacgcagt
     4201 cctcgagcac caggcaatcg tagccgcggt cgttcgcctc gcgcaccgtg gtgttcacgc
     4261 acacctcggt ggtcacgccg cacaccacca ggctgcggat gccgcggttg cgcagcatcg
     4321 agtcgaggtc ggtggcgtag aaggcgccct tgccgggctt gtcgaccacc ggctcgccgg
     4381 gcaggggctt gaggtcgtcg acgatgtcgt ggccgtactc gccgcgcacc aggatgcggc
     4441 ccatcgggcc gggatcgccg atgccgacct tcaggcgccc gcgcagcttc ttgctctgcg
     4501 gcaggtcgga gagatcggcg cggtggcctt cacgcgtgtg gatcacgagc agcttgcggt
     4561 tgcgcgcggc ctccagcact ttcttcgtcg gcgcgatcgc cttgcgcaag agcgagacgt
     4621 cgttgccgag cgcctcgccg aagccgcccg ggtcgacgaa gtcgcgctgc atgtcgatga
     4681 tcaccagcgc gcaggcgcgc gcgtcgaact cgaactcgta aggctccgcc tggaccctgg
     4741 ccatcgcccc tccctctgcc tcgtcaatgt actttcataa ggaacggaaa tccattcagg
     4801 atgattctga atgaatggtg ggccgctacc caattaaggt gacgggctag aggaggaggg
     4861 aatcatgaaa cctgctgttc gagcggcgtt tgccgtcctg ttgcctgtcg ccctcgccgg
     4921 gatcggcgag ccggcggaag cgcgcattac caagatcgag atcacttccc gcgccatcgc
     4981 tttcggcggc gcctcgttcg ggtccgtggg ccagtacgag acgctgcgcg gggtggcgta
     5041 cggggaggtc gacccgaacg atccgctgaa tgaagtgatc acggacatca agctcgcccc
     5101 gcgcaactcg cgcggcaagg tcgagtacaa catggacgtc tggatcaaca agccgctcga
     5161 catgacgcgc gcgaacggca cgctgctgca cgacgtgccc aatcgcggca acgtgcgcag
     5221 cctcgagatc aacgtcggcg gcgcggccga cctcaacgcc ggcgacggct ggctgcagcg
     5281 ccagggctac gtcatgtcgg acaacggctg gcagggcgac gtgtccaccg ggctgcagat
     5341 ccggctgccg atcgcgcgga acaagaacgg cacgccgatc gtcaaccgca tccgggccga
     5401 atacatccag ggctcgccga ccagcacggt cgacatcacc acgccgccct cctacgaggc
     5461 ggtgagcacc aataacgccg gcgcgacgct gacgcggcgc gtgcaccagg acgatcccaa
     5521 ggagctcatc ccgaacgccg actgggcgtt cgccgactgc tcgacggtgc cgtttcccgg
     5581 cgtgccgagc acgaccaagg tgtgcctgaa gggcaacttc gacaccaacc atatctacga
     5641 gttgctctac gacgcgaaga acccgacggt cctcggcttg ggcttcgcgg cgacgcgcga
     5701 cttcgtctcg ttcctgcgca acagcaacgg cgcgggcagc gactgcaggg acgacgacca
     5761 cgggcatcac gggcaccacg gcaagcgcca cgacgacgac gatgacgacg atgacgacgg
     5821 cggcgaccga cgccacgggc cctgccgcgt gaaagtccag atgcccgaga acccgctcgg
     5881 aagatcgatc cagaacgcga tcatctacgg ctcgtcgcag agcggacgct acatccgcac
     5941 cttcatccag ctcggcttca accgtgacga gaaccacaac atggtgttgg agggcgcgat
     6001 cccgcacaag gcgtcgaacc gcggcgcgtt caacgtgcgc ttcggccagc ccgaccgcct
     6061 ttcgggcacg cagcacaccg agcgccagtt cccgggcggc gagtcgtcgc agacctgggg
     6121 cgagtaccac gaccgcatct cgggcgtgaa ggcgggccag ctcgaccgct gccgcaagac
     6181 cgaccagtgc ccgaagatct tccacaccaa caccgacacc gagtactggc aggcgctgat
     6241 ggcgctgaac accaccaact cggagggcac ggaagatctg aagatcccga gcaacgtgcg
     6301 catctacctc ttcgcgggca cgcgccacgg cggcggcaac gtgctggcgc agccgccggc
     6361 gacggtgccg gcgccgcccg cgggctgcca gatgccgaac aacagcaacc cgttcatcca
     6421 cggccagcgc gccctgctgg tggcgctgcg cgcctggatc gtggacggcg tccggccgcc
     6481 gtcgagcctg tatccgaagc tgcgcgacgg cacgctggcg cggctgcggg acatcgacta
     6541 cccgtacctc cccgcggtga acttcacgct cgccggggtc acgacgcaga agttctacct
     6601 cgatcgcggg ccgctcttca acgtcgagga catctccggg gtgatgcgcg agccgccgat
     6661 caagcgcggc gcctacccgg tgcgccagcc gcaggtggac caggacggca acaccatcgc
     6721 cggcctgccg agcaccgcca tgctggttcc gctcggcacc tacctcggct ggaacgtgag
     6781 gcgcgcgggc tttagcgaag gcgattcctg cgacctgaac ggggcgtacg tgccgttctt
     6841 ccgcaccctg gcccagcgcc aggcggcggg cgatccgcgg ccctccctcc aggagcgcta
     6901 cccgacccac gccgactacg ttgccaaggt caccgcggca gcgaacagcc tcgtcgcccg
     6961 gcgcctcctg ctgcccgagg acgcggcttt cctgatcaac gcggcgaacg cggccgcg
//
LOCUS       DATBSZ010000279         9897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_398113, whole genome shotgun sequence.
ACCESSION   DATBSZ010000279 DATBSZ010000000
VERSION     DATBSZ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398113"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VIG70_18105"
     CDS             complement(<1..316)
                     /locus_tag="VIG70_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt efflux protein"
                     /protein_id="HEY8252511.1"
                     /translation="MNDPSRPSLLERLSSLIMREPGDRAELTQLLRAAYSRNLLDADA
                     LSIIEGALTVSEMAVRDIMIPRAQVDFIDINEPVETFVKQVISTAHSRFPVIDQNRDD
                     VIG"
     gene            complement(333..716)
                     /gene="ybeY"
                     /locus_tag="VIG70_18110"
     CDS             complement(333..716)
                     /gene="ybeY"
                     /locus_tag="VIG70_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY8252512.1"
                     /translation="MSRRGVPGPSSLRRWARAASAGQVHLRIVGEGEGRRLNRIYRRK
                     NRATNVLTFHTGDVVLCHPVIRREARAQGKSLAAHYAHLVVHGCLHLRGYDHEKTRDA
                     ARMEAREIAILRRGGIHNPYAPTVE"
     gene            complement(736..1683)
                     /locus_tag="VIG70_18115"
     CDS             complement(736..1683)
                     /locus_tag="VIG70_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY8252513.1"
                     /translation="MNRAKPVELKLDPVDNQRLARLCGALDENLRQIESALDVTIARR
                     GAKFTVRGAQAERAAHALEHFYGRASGDLSIEDVQLGLTELSQKTAPEQGTGPQLLTR
                     RADLHGRTPHQIEYIEKILAHDLTLGIGPAGTGKTYLAVACAVDALERDKVKRIVLVR
                     PAVEAGERLGFLPGDLAQKVDPYLRPLYDALFDLMGFEKTGKLLERGTIELAPLAYMR
                     GRTLNHSFIILDEAQNTTPEQMKMFLTRIGFGAKAVVNGDVTQIDLGRDKKSGLIEAR
                     RILAGVRGIAFTQFGAQNVMRHPLVARIIDAYDKHAQEE"
     gene            complement(1680..2993)
                     /gene="miaB"
                     /locus_tag="VIG70_18120"
     CDS             complement(1680..2993)
                     /gene="miaB"
                     /locus_tag="VIG70_18120"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016726663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY8252514.1"
                     /translation="MKKLFLRTFGCQMNAYDSDKISDVLREAEGLELTPDPADADVIV
                     FNTCSVREKAQEKVFNDLGRVKHLKRSRPGLMIAVGGCVASQEGPAIVERAPYVDVVF
                     GPQTLHRLPQLLERRRATGQPQVDVSFPEIEKFDHLPPARVDGASAFVSIMEGCSKYC
                     SFCVVPYTRGEEVSRPFEDVVAEIAGLVGQGVKEVTLLGQNVNAWRGTIDGTPADFAE
                     LLHYLCEMQLERIRYTTSHPREFTQRLIDAHAALDRLAPHVHLPVQSGSDRVLAAMKR
                     GYTALEYRSIVRRLRQARPDISLSSDFIVGFPGETEADFAATLRLACEAEFDASFSFI
                     YSPRPGTPAAELPDGVSHSEKLERLQRLQAQLEAQAKAVAEGMVGTTQRVLVEGPSKR
                     NPAELAGRTGNNRVVNFPGGPGLHHSFVDLRITAALSHSLRGELV"
     gene            complement(2990..3298)
                     /locus_tag="VIG70_18125"
     CDS             complement(2990..3298)
                     /locus_tag="VIG70_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252515.1"
                     /translation="MKGLVVLLAFAGFSTAAMAQLRTIPKEAQPGVIRHLESMVVEID
                     GKPRELSPGAQIRDPDNRIVLPGALAEKSQARYLVDGTGKVFRVWILSPREVESSFPK
                     "
     gene            complement(3315..3734)
                     /locus_tag="VIG70_18130"
     CDS             complement(3315..3734)
                     /locus_tag="VIG70_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8252516.1"
                     /translation="MECKVRWTGEGMSFVAETGSNHLVTMDGAPDGGGRNLAPRPMEM
                     VLAGTGGCTAYDVVVILKKSGQDVTGCEVKLTAERATTDPKVFTKIHMHWTVRGRGLK
                     RNIVENAVKLTHEKYCSATAMLIKTAEITRDFEVVEA"
     gene            3795..4223
                     /gene="rplM"
                     /locus_tag="VIG70_18135"
     CDS             3795..4223
                     /gene="rplM"
                     /locus_tag="VIG70_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY8252517.1"
                     /translation="MTTYTAKAGQVEHGWYVVDAQDKVLGRLASQIAVRLRGKHKPIY
                     TPHVDTGDYIVVVNAAKLRVTGRKSERKIYYRHSGQPGQLHQTNFAKLHAKKPERVLQ
                     LAVKGMLPKGPLGYAMIKKLKVYGGATHPHSAQQPKALEI"
     gene            4228..4620
                     /gene="rpsI"
                     /locus_tag="VIG70_18140"
     CDS             4228..4620
                     /gene="rpsI"
                     /locus_tag="VIG70_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY8252518.1"
                     /translation="MVGDYYYGTGRRKSAVARVFLKPGKGEFQVNGKPVDQFFARETG
                     RMVVRQPLELTKNLSAFDILVNVYGGGESGQAGAVRHGISRALIEYDAALKPTLSAAG
                     LVTRDAREVERKKVGLHKARRRKQYSKR"
     gene            4681..5721
                     /gene="argC"
                     /locus_tag="VIG70_18145"
     CDS             4681..5721
                     /gene="argC"
                     /locus_tag="VIG70_18145"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY8252519.1"
                     /translation="MIKAGIVGGTGYTGVELLRLLVQHPQVELKAITSRKEAGTPVAQ
                     MFPSLRRRVELAFVEPSARALAGCDVVFFATPNTVAMGEARAVLEGGARIVDISADFR
                     IRDVAEWERAYKTKHAAPELVREAVYGLPEMNREQVRRARLVANPGCYPTSVQLGFLP
                     LVEAGVVDVEHLIADAKSGVSGAGRKTELSLMFAEASDNFAAYNVMGHRHWPEIRQGL
                     AQAAKRDVGLVFTPHLTPLIRGIHATLYARITREVDFQQLFEKRYAGEPFVDVMPAGS
                     HPDTRSVRSANTCRLAVHRPPEGAKRSDTLVVLSVIDNLVKGASGQAVQNMNLMFGLA
                     ETTGLEQVAVVP"
     gene            5721..6476
                     /locus_tag="VIG70_18150"
     CDS             5721..6476
                     /locus_tag="VIG70_18150"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEY8252520.1"
                     /translation="MASGRWKAVRQRFGIAAPRVEVQTPIPWYWRWAGIAVLLGVAAA
                     AGSWLYDAGRRFAGFDRSEVVQELTRVQGELAGARTELARLRAIANAADSKVSIERTA
                     QQKLAQQVRGLEQELARVREELAMVEQMLTSEERHTQSLSIYQFKVSPDVLPGEYRYR
                     LLLLTPTDRRGRDFSGRLELVVSLQEGGQSAMMSFPEPGDAAAAAFRLAFKYFRRVEG
                     TFRVNPKAKVESVQVRVYETGSNQPRATHSVTL"
     gene            6503..6904
                     /locus_tag="VIG70_18155"
     CDS             6503..6904
                     /locus_tag="VIG70_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY8252521.1"
                     /translation="MFNKTSKPQNRIDSLIGATTRIEGNVIFSGGLRVDGMVRGNVAA
                     LPDQPGTLVVSSEARIDGEVQAAHIVVNGTIHGPVHATETLELQPGSRVKGDVYYKSI
                     EIQQGAVVEGRLVHHAAEVKGVELKLASDRG"
     gene            6914..7294
                     /gene="erpA"
                     /locus_tag="VIG70_18160"
     CDS             6914..7294
                     /gene="erpA"
                     /locus_tag="VIG70_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY8252522.1"
                     /translation="MNAITETVTETPPVAEMPAPLVFTDSAANKVKELITEEGNPELK
                     LRVFVTGGGCSGFQYGFTFDEVKNEDDAAMEKNGVTLLIDPMSYQYLVGSEIDYQEGL
                     EGAQFVIKNPGAKSTCGCGSSFST"
     gene            complement(7356..8576)
                     /locus_tag="VIG70_18165"
     CDS             complement(7356..8576)
                     /locus_tag="VIG70_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8252523.1"
                     /translation="MSRVVVVVLLAASAVVAFATIGPSAERSVEPPKRPIIEALPIRP
                     DAAPAPKSYVREELYQRGDTLAALIERLGIEPPHAARLAKLRVLQQLRPGTHVSAEVS
                     ADGEPLALSFLSGRDTLVKIAAEANEQFRVTDDRAQLYTRVAMKSGVVQSSLFAAADE
                     AGIPDGIAMQLGDVFAGDIDFHRDLRKGDHFAVVYELHYLGGRPVRAGRVLAAEFTNN
                     RKTFRAVHFGNGYYTPDGKNLRKAFMRSPLEFSRVSSGFGMRRHPIAQAWIKHQGIDY
                     AAPTGTRVRAVGDGIVETAGLKGGYGKVVILRHQGQTTTVYAHLSRINVQRGQRVAQN
                     DTIGAVGQTGWATGPHLHYEFRIAGEARNPFSIAMPAAHPVAQQDLPAFRNHAAPLIT
                     RLDLIANSALAQLE"
     gene            8668..9852
                     /gene="tyrS"
                     /locus_tag="VIG70_18170"
     CDS             8668..9852
                     /gene="tyrS"
                     /locus_tag="VIG70_18170"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY8252524.1"
                     /translation="MSAIGQAIQTIRRGADELIVEEELAKKLASGRKLRIKLGLDPTA
                     PDLHLGHTVVINKLRDFQRLGHQVQFLIGDFTGMIGDPTGKNQTRPPLTREAIQQNAK
                     SYQDQVFKILDPASTQILYNSEWSDKLGAEGMIRLASRYTVARLLERDDFTKRLKSNQ
                     PIALHELLYPLMQGYDSVAMKADVELGGTDQKFNLLVGRELQKQYGQEPQCILTMPLL
                     EGTDGVEKMSKSLGNYIGIAEPPQEIFGKLMSISDDLMWRYIDLLSFEPPEKIKAWKS
                     EHPRDVKVRFAKEIVARFHSAKAGEQAQADFESRFREGEMPADMPEVTIKAGDGGLLI
                     AQLLKQANLTPSTSEAQRMIEQGGVKLDGERVSDKGLKIPAGRTVVAQVGKRKFARIT
                     IK"
BASE COUNT         1735 a         3136 c         3365 g         1661 t
ORIGIN      
        1 cgccgatcac gtcgtcgcgg ttctggtcga tcaccgggaa gcgcgagtgc gcggtggaga
       61 tcacctgctt gacgaaggtc tcgaccggct cgttgatgtc gatgaaatcc acctgggcgc
      121 gcgggatcat gatgtcgcgc actgccatct ccgacacggt gagcgcgccc tcgatgatcg
      181 agagcgcatc ggcgtcgagc aggttgcgcg agtacgccgc tctcaggagc tgcgtgagct
      241 cggcccggtc gccgggctcc cgcatgatga gcgacgacag gcgctcgagc aggctgggtc
      301 gggaaggatc gttcatgctt ggcgcgggga tctcattcta ctgttggcgc gtagggattg
      361 tggattccgc cgcgccgcag gatggcgatc tcgcgcgctt ccatgcgcgc agcatcccgt
      421 gtcttttcat gatcgtagcc gcgcaggtgc aggcatccgt gcaccacgag gtgcgcgtaa
      481 tgcgcggcga ggctcttgcc ttgcgcgcgc gcctcgcgcc ggattaccgg gtggcacagc
      541 acgacgtctc cggtatgaaa ggtcaggacg ttggtcgcgc ggttcttcct tcggtaaatg
      601 cggttgagcc gccggccctc gccttcgccg acgatgcgca gatggacttg gcccgcagac
      661 gcggcgcgcg cccagcggcg caaggacgac ggacccggca ctccgcgccg cgacactgcg
      721 tactggatgg aaacgctact cttcctgcgc gtgcttgtcg taggcatcga tgatgcgcgc
      781 gaccagcgga tggcgcatca cgttctgtgc gccgaactgc gtgaaggcga tgccgcgcac
      841 gcccgcgagg atgcggcgcg cctcgatcag gccgctcttc ttgtcgcgtc cgaggtcgat
      901 ctgcgtgacg tctccgttca ccaccgcctt cgcgccgaag ccgatgcgcg tgaggaacat
      961 cttcatctgc tcgggcgtgg tgttctgcgc ctcgtcgagg atgatgaacg aatggttgag
     1021 cgtgcgcccg cgcatgtagg ccagaggagc caattcgatg gttccgcgct cgagcagctt
     1081 gccggtcttc tcgaatccca tcaggtcgaa gagagcgtcg tacagtggtc gcaggtaagg
     1141 gtcgaccttc tgcgcgaggt cgccgggcag gaagccgagg cgctcgccgg cctcgaccgc
     1201 cggtcgcacc agcacgatgc gcttcacctt gtcgcgctcg agcgcgtcca ccgcgcaggc
     1261 cacggcgagg taggtcttgc cggtgcccgc ggggccgatg ccgagggtga ggtcgtgcgc
     1321 caggattttc tcgatgtact cgatctggtg cggcgtgcgg ccgtgcaggt cggcgcgccg
     1381 cgtgagcagc tgcgggcccg tgccttgctc cggcgcggtt ttctgcgaaa gctcggtgag
     1441 cccgagctgc acgtcttcga tgctcaggtc gcccgaagcg cggccgtaga agtgctccag
     1501 ggcgtgcgcc gcgcgctcgg cctgggcgcc gcgcacggtg aacttcgcgc cgcggcgcgc
     1561 gatcgtcacg tcgagcgccg attcgatctg gcgcaggttc tcgtcgagcg cgccgcacag
     1621 gcgcgcgagg cgctggttgt cgaccggatc cagcttgagt tcaaccggct tggcccgatt
     1681 cagacgagct cacctcgaag cgaatgggac aaggcagcag tgatgcgaag gtccacgaac
     1741 gaatgatgga gtcccgggcc gccgggaaag ttcaccacgc gattgttgcc ggtgcggccg
     1801 gcgagctcgg ccgggttcct tttcgacggg ccttccacca ggacgcgctg ggtagtgccg
     1861 accatgcctt cggcgacggc cttggcctgg gcttcgagct gcgcttgcag gcgctgcaac
     1921 ctttcaagct tctccgaatg agaaactccg tctggtagtt cggccgcagg cgttcccggg
     1981 cgcgggctgt agatgaagga gaacgaagcg tcgaattcgg cctcgcacgc gagcctgagc
     2041 gtcgccgcga aatcggcctc ggtctcgccg ggaaagccga cgatgaagtc cgacgacagg
     2101 ctgatgtccg gccgcgcctg gcgcagccgg cgcacgatcg agcggtattc gagcgcggta
     2161 tagccgcgct tcatcgcggc gagcacgcgg tcggatcccg actgcaccgg caggtggaca
     2221 tgcggggcga gccggtcgag cgcggcgtgc gcgtcgatca gccgctgcgt gaattcgcgc
     2281 ggatgggacg tcgtgtagcg gatgcgctcg agttgcattt cacagaggta atgcaacaac
     2341 tcggcaaaat cggccggcgt gccgtcgatc gtgccgcgcc aggcgttcac gttctggcca
     2401 agaagcgtaa cctccttgac gccttgccca acgagaccgg cgatctcggc gaccacgtcc
     2461 tcgaaaggcc gcgaaacctc ctcgccgcgc gtgtagggca cgacgcagaa gctgcagtac
     2521 ttgctgcagc cctccatgat cgagacgaac gcggaggcac cgtccactct ggcgggtggc
     2581 aggtggtcga acttctcgat ttcggggaag ctcacgtcga cctgcggttg gccggtggcg
     2641 cgccggcgct cgaggagctg cggcaggcgg tgcagcgtct gcggcccgaa caccacgtcg
     2701 acatacggcg cgcgctcgac gatcgccggc ccctcctggc tcgcgacgca gccgccgacg
     2761 gcgatcatca gccccggccg cgagcgcttc agatgcttga cgcggccgag gtcgttgaac
     2821 actttctcct gcgccttctc gcgcacggag caggtgttga agacgatcac gtcggcatcc
     2881 gccgggtccg gcgtgagctc gagcccctcg gcctcgcgca gcacgtcgga gatcttgtcc
     2941 gagtcgtacg cgttcatctg gcagccgaag gtgcgcagga agagcttttt catttcggaa
     3001 aagaggattc gacttcgcgc ggcgaaagga tccagacgcg aaacaccttg ccggtgccgt
     3061 cgacgaggta gcgcgcctgc gatttctcgg cgagcgcgcc cggcaacacg atccggttgt
     3121 ccgggtcgcg gatctgcgcc cccggggaga gctcgcgcgg ttttccgtcg atctcgacca
     3181 ccatcgactc gaggtggcgg atcactcccg gttgcgcctc cttggggatg gtgcgcagct
     3241 gggccatggc agcagtgctg aatcctgcaa acgcgaggag gacgacaaga cccttcatga
     3301 gcgggattgt cgcactaggc ttcgaccacc tcgaaatccc gggtgatttc agcggtcttg
     3361 atgagcatcg cggtagccga gcagtatttc tcgtgcgtta acttcaccgc gttttccacg
     3421 atatttcgct tgagcccgcg gccgcgcacc gtccagtgca tgtggatctt ggtgaacacc
     3481 ttggggtcgg tcgtcgcgcg ctccgcggtc agcttgacct cgcagccggt cacgtcctgg
     3541 ccgctcttct tgaggatcac caccacgtcg taggcggtgc agccgccggt gccggcgagc
     3601 accatctcca tcggccgcgg cgccaggttc cgcccgccgc cgtcgggcgc gccgtccatc
     3661 gtcaccaggt ggttgctccc ggtctcggca acgaagctca tgccctcgcc ggtccagcgc
     3721 actttgcatt ccatagggac tggaattcta gtaaaatccg cggcttttct cgattcggcg
     3781 caggaaccac ccgcatgaca acctacaccg cgaaagcagg ccaggtcgag cacggctggt
     3841 acgtggtcga cgcccaggac aaggtcctcg gccgccttgc ttcgcagatc gccgtgcgcc
     3901 tgcgcggcaa gcacaagccc atatacaccc cgcatgtcga tacgggcgac tacatcgtcg
     3961 tggtgaacgc cgcgaagctc cgcgtgaccg gccgcaagtc ggagcgcaag atctactacc
     4021 ggcacagcgg gcagccgggg cagctacacc agacgaactt cgccaagctg cacgcgaaga
     4081 agcccgagcg cgtgctgcag ctggcggtaa agggcatgct gcccaagggg ccgctcggct
     4141 acgcgatgat caagaagctg aaggtctacg gcggcgccac acatcctcat tcggctcagc
     4201 agccgaaagc tttggagatc taacgcaatg gtcggtgact actactacgg cacggggcgg
     4261 cgcaagagcg cggtcgcgcg cgtattcctc aagcccggca agggcgagtt ccaggtgaac
     4321 ggcaagccgg tcgatcagtt cttcgcccgc gagaccggcc gcatggtggt gcgccagccg
     4381 ctcgagctca ccaagaacct gtcggccttc gacatcctgg tgaacgtgta cggcggcggc
     4441 gagagcggcc aggcgggcgc ggtgcgccac ggcatctcgc gcgcgctgat cgagtatgat
     4501 gcggctctaa aaccgacgtt gagcgcggcc ggactggtga cgcgagacgc gcgcgaggtc
     4561 gagcgaaaga aggtcggcct gcataaagca aggcggcgca agcagtactc gaaacgctga
     4621 agttcaggga ttctgcgaac cgaaagccgc caattggcgg cttttgtttt tgggtgacga
     4681 atgatcaagg caggcatcgt cggcggtacg gggtacacgg gcgtggagct cctgcgtctg
     4741 ctcgtccagc atccgcaggt cgagctgaag gcgatcactt cgcgcaagga ggcgggcacg
     4801 ccggtggcgc agatgttccc gagcctgcgg cgccgcgtcg agctcgcctt cgtcgagccg
     4861 agcgcgaggg cgctcgccgg ttgcgacgtg gtgttcttcg ccacgccgaa tacggtcgcc
     4921 atgggcgaag cgcgcgcggt gctcgaaggc ggcgcccgca tcgtcgacat ctccgcggac
     4981 ttccgcatca gggacgtggc ggaatgggag cgcgcctata agacgaagca tgcggcgccg
     5041 gagctggtgc gcgaagcagt ctatgggctc cccgagatga atagagaaca ggtgcggcgc
     5101 gcgcgcctcg tcgcgaaccc cggttgctac ccgacctcgg tgcagcttgg cttcctgccg
     5161 ctggtggagg cgggcgtggt cgacgtcgag cacctgatcg ccgatgcgaa gtccggcgtg
     5221 agcggcgccg ggcgcaagac cgagctcagc ctgatgttcg cggaagcttc cgacaacttt
     5281 gccgcgtaca acgtcatggg acatcgccac tggcccgaga tccgccaggg cctcgcgcag
     5341 gcggcgaagc gcgacgtcgg gctcgtgttc accccgcatc tcacgccgct catccgcggc
     5401 atccatgcca cgctgtatgc aaggattacg cgcgaagtgg atttccagca gctgttcgag
     5461 aagcgctacg ccggcgagcc cttcgtcgac gtgatgccgg ccggcagcca tcccgacacg
     5521 cgctcggtgc gctcggcgaa cacctgccgc ctcgccgtgc atcgtccgcc ggagggtgcg
     5581 aagcgctccg atacgctggt ggtgctgtcg gtgatcgaca acctggtaaa gggcgcgtcc
     5641 ggccaggcgg tgcagaacat gaatctcatg ttcggcctcg ccgagacgac cggcctcgag
     5701 caggtagcgg tggttccata atggcctcgg gtcgctggaa ggccgttcgt cagcgcttcg
     5761 gcattgcggc gccgcgcgtc gaggtgcaga cgccgatccc gtggtactgg cgctgggctg
     5821 gcatcgcggt cctgctcggc gtcgccgccg ccgccggttc ctggctctac gacgccggca
     5881 ggcgcttcgc cggtttcgat cgcagcgagg tggtccagga gctgacgcgc gtgcagggcg
     5941 agcttgcggg tgcgcgcacc gagcttgcgc ggctgcgcgc catcgccaat gccgccgaca
     6001 gcaaggtctc gatcgagcgc actgcgcagc agaagctcgc tcagcaggtg cggggcctcg
     6061 agcaggagct tgcccgcgtc cgcgaggagc tcgcgatggt cgagcaaatg ctcacatcgg
     6121 aagaacgtca cactcagagc ctctccatct atcaattcaa ggtctcgccc gacgtcctgc
     6181 cgggggaata ccgctatcgg ctgctgcttt tgacccccac cgaccggcgc gggcgcgact
     6241 ttagcggcag gctcgagctc gtcgtaagcc tgcaggaggg ggggcagagt gctatgatgt
     6301 ctttcccgga gcctggggat gccgcagctg cggctttccg gttggctttc aagtacttcc
     6361 gacgggtcga agggactttc cgggtcaacc cgaaggcgaa ggtcgaaagc gtgcaggtcc
     6421 gcgtttacga gaccggttcc aaccagccac gcgcgacgca ttcggtaact ttgtagagca
     6481 gcgaggggag agggagatag aagtgttcaa caagacgagc aaaccgcaga accggatcga
     6541 cagcctgatc ggcgccacga cgcgcatcga gggcaacgtg attttcagcg gcgggctgcg
     6601 cgtggacggc atggtccgcg gcaacgtcgc ggcgctgccc gaccagccgg gtacgctggt
     6661 ggtttcgtcg gaggcgcgca tcgacggcga ggtccaggcc gcgcatatcg tggtgaacgg
     6721 cacgatccac gggccggtgc atgcgaccga gacgctcgaa ctacagcccg gctcgagggt
     6781 caaaggagat gtgtattaca agagcatcga gatccagcag ggcgcggtcg tcgaggggcg
     6841 cctggtgcat cacgccgccg aggtgaaggg cgtcgagctc aagcttgcat ctgacagagg
     6901 ataaggaaca ggtatgaacg caatcaccga aacagtcacc gaaactccgc cagtcgccga
     6961 aatgccggcg ccgctggtct tcaccgacag cgctgcgaac aaggtcaagg agctgatcac
     7021 cgaggaaggc aacccggagc tcaagctccg cgtgttcgtc accggcggcg gctgctctgg
     7081 cttccagtac ggcttcacct tcgacgaggt gaagaacgag gacgacgccg cgatggagaa
     7141 gaacggcgta acgctcctca tcgacccgat gagctaccag tacctggtgg gctccgagat
     7201 cgactaccag gaaggcctcg agggtgcgca gttcgtgatc aagaaccccg gcgcgaagag
     7261 cacctgcggc tgcggctctt ccttctccac gtaggcgaga cactcaccaa cgaaaaaggg
     7321 ccctgacggg cccttttgtt ttcccggtcc tctttctatt cgagctgcgc gagcgcgctg
     7381 ttggcgatga ggtcgagccg cgtaatcagc ggcgcggcgt gattgcggaa ggccggaagg
     7441 tcctgctgcg caaccgggtg cgcggcgggc atggcgatcg agaacgggtt gcgcgcctcg
     7501 ccggcgatgc ggaactcgta gtgcaggtgc ggtccggtcg cccagccggt ctggccgacg
     7561 gcaccgatgg tgtcattctg cgcgacgcgc tgaccacgtt ggacattgat gcggctcagg
     7621 tgcgcgtaca ccgtggtggt ctggccttga tggcgcagga tgaccacctt gccgtagccg
     7681 cccttcaggc cggcagtctc gacgattccg tcgcccaccg cgcgcacgcg cgttccggtc
     7741 ggcgctgcat agtcaatgcc ctggtgcttg atccaggcct gcgcgatcgg atggcggcgc
     7801 atgccgaagc ccgagctcac gcgcgagaac tccagcggcg agcgcatgaa ggccttgcgc
     7861 aggttcttgc cgtcgggcgt gtagtagccg ttgccgaaat gcaccgcgcg gaaagtcttg
     7921 cggttgttgg tgaactcggc ggcgagcacg cggccggcgc gcaccgggcg cccgccgaga
     7981 tagtggagct cgtagaccac cgcgaaatga tcgcccttgc gcaggtcgcg gtggaagtcg
     8041 atgtcgccgg cgaagacgtc gccgagctgc atggcgatgc cgtccggaat gcctgcttcg
     8101 tcggcggcgg cgaagagcga gctctgcacc acgcccgact tcatggcgac gcgcgtgtag
     8161 agctgggcgc gatcgtcggt cacacggaat tgctcgtttg cctcggcggc gatcttgacg
     8221 agcgtgtcgc gccctgagag gaagctgagc gcgagcggct cgccgtcggc gctcacctcg
     8281 gcgctgacgt gcgtaccggg gcgcagttgc tggagcacgc gcagcttcgc gaggcgggcc
     8341 gcgtgcggcg gttcgatgcc gaggcgctcg atcaacgcgg ccagcgtgtc gccgcgctgg
     8401 tagagctctt cgcgaacgta ggatttcggc gccggcgcgg cgtccgggcg gatcggcagc
     8461 gcttcgatga tcggccgctt cggcggctcg acgctgcgct cggccgaagg accgatggtc
     8521 gcgaacgcca ccaccgcgga agcggcgagg agcaccacca cgacgacccg gctcaagcgg
     8581 ccgtaaattc ccatgaatcg gctagaatcc cttgaatttc agaagcctgc ggagtctagc
     8641 aaacaacccg ccgcagtgaa atcaactatg tccgccatcg ggcaggccat tcagaccatc
     8701 cgtcggggtg ccgacgagct gatcgtcgag gaagagttgg cgaagaagct cgccagcggc
     8761 cgcaagctcc ggatcaagct cggactggac cccaccgccc ccgacctgca tctcggccat
     8821 accgtcgtaa tcaacaagtt gcgtgatttc cagcgcctgg gccaccaggt gcagttcctg
     8881 atcggggact tcaccgggat gatcggcgac ccgaccggga agaaccagac gcgcccgccg
     8941 ctgacgcgcg aagcgattca gcaaaatgca aaaagttatc aggaccaggt tttcaagatc
     9001 ctcgacccgg ccagcaccca gatcctctac aactcggaat ggtcggacaa gctcggcgcc
     9061 gagggcatga tccggctcgc ttcgcgctac acggtggcgc gcctgctcga gcgcgacgac
     9121 ttcaccaagc gattgaagag caaccagccg atcgcgctgc acgagctgct ctatccgctg
     9181 atgcagggct acgactcggt ggcgatgaaa gccgacgtgg agctcggcgg caccgaccag
     9241 aagttcaacc tgctcgtcgg ccgcgagctg cagaagcagt acgggcagga accgcaatgc
     9301 atcctcacca tgccgctgct cgagggcacc gacggcgtgg agaagatgtc caagtcgctc
     9361 ggcaactaca tcggcatcgc cgagccgccg caggagatct tcggcaagct gatgtccatt
     9421 tcggacgatc tgatgtggcg ctacatcgat ctgctttcct tcgagccgcc ggaaaagatc
     9481 aaggcgtgga aaagcgagca tccgcgcgac gtgaaagtgc gcttcgccaa ggagatcgtg
     9541 gcgcgcttcc acagcgcgaa agccggcgag caggcccagg cggatttcga aagccgcttc
     9601 cgcgagggcg agatgccggc ggacatgccg gaggtcacga tcaaggcggg cgatggtggc
     9661 ctccttatcg cgcagctcct caagcaggcc aacctcacgc cgagcacctc ggaggcgcag
     9721 aggatgatcg agcagggggg tgtgaagctc gacggtgagc gcgtgtcgga caaggggctc
     9781 aagatccccg ccgggcgcac cgtagtggcg caggtgggca agcgcaagtt cgcccgcatc
     9841 acgatcaagt gacaagagtg gcggaaaccc tcgcctatga ggtctacgct ctcaaat
//
LOCUS       DATBSZ010000280         4177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2038007, whole genome shotgun sequence.
ACCESSION   DATBSZ010000280 DATBSZ010000000
VERSION     DATBSZ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2038007"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1919)
                     /locus_tag="VIG70_18175"
     CDS             complement(<1..1919)
                     /locus_tag="VIG70_18175"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY8252525.1"
                     /translation="MAAGLIFALVCAVLAIVYGAWQATRILRLPDGNERMREIAAAVR
                     EGAIAYLWRQYKTIAIVGVILFLVIGFVPQLGWATAWGFLLGAVLSGASGIIGMNISV
                     RANVRTAEAARVGLNEALQVAFRGGAVTGLLVVGLGLLGVAGYYALLYANAHDKANLA
                     QLIHPLVGLAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIAD
                     NVGDNVGDCAGMAADLFETYAVTIIATMLLGALMVQTQSPAALVYPLALGGFAIIASI
                     AGTWFVRAKPGEVNVMPALYRGLWVAGGLALVAFWPITLYVMGDVLRNVPFEIGGAKK
                     LITVWHLWGAAVVGLALTGFLVWITEYYTGTQFKPVRHVAEASVTGHATNIIAGLGVS
                     MKSTAWPVIAVCVAILISYSLAGLYGIATAATSMLSMAGIIVALDAYGPITDNAGGIA
                     EMADLPDSVRAITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHALEAAGKTLS
                     FDLSNHMVIIGLFIGGMVPYLFGAMAMEAVGRAAGAVVLEVRRQFKEIPGIMQGTAKP
                     QYGVAVDMLTRAAIREMMIPSLLPVLVPILVGLLLGPQALGGVLMGTIVTGLFVAISM
                     CTGGGAWDNAKKYIE"
     gene            complement(1974..2609)
                     /gene="adk"
                     /locus_tag="VIG70_18180"
     CDS             complement(1974..2609)
                     /gene="adk"
                     /locus_tag="VIG70_18180"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY8252526.1"
                     /translation="MRLILLGPPGAGKGTQAKFIREKFGIPQISTGDMLRAAVKAGTP
                     LGTEVKKVLDAGQLVSDDIIIDLVKERLKAPDCRAGYLFDGFPRTIRQAEEMGEAGVA
                     IDYVLEIDVPDDEIITRMSGRRVHPGSGRVYHVKFNPPKTKGRDDVTGEPLVLRDDDR
                     EETVKRRLEIYRTQTRPLVDYYQRLAPDKYRKISGRGSLDEVTKRAFAALS"
     gene            complement(2609..3331)
                     /gene="kdsB"
                     /locus_tag="VIG70_18185"
     CDS             complement(2609..3331)
                     /gene="kdsB"
                     /locus_tag="VIG70_18185"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEY8252527.1"
                     /translation="MRFQVIIPARYASTRFPGKPLAEIGGKPMVVHVCERAARSGAAA
                     VHVATDDQRIAAAVRAHGHQAIMTRADHASGTDRLAEAARKLRLKDSSIVVNVQGDEP
                     LIAPRLIAQVARILERKPRASVSTACHAIHDAASLANPNVVKVVLDAEGYALYFSRAQ
                     IPHPREPGATCYRHAGIYGYRVSFLKKYSRLKASPLEKAEALEQLRVLWHGHRIAVAV
                     SETEIPPGVDTPQDLEAVRRII"
     gene            complement(3328..3531)
                     /locus_tag="VIG70_18190"
     CDS             complement(3328..3531)
                     /locus_tag="VIG70_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY8252528.1"
                     /translation="MSAVDPRLLEILVCPLCKGKLIYRKNAPQHAQELVCKADRLGYP
                     VKDGIPVMLEEEARKLPPEEEVS"
     gene            complement(3503..>4177)
                     /gene="lpxK"
                     /locus_tag="VIG70_18195"
     CDS             complement(3503..>4177)
                     /gene="lpxK"
                     /locus_tag="VIG70_18195"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY8252529.1"
                     /translation="EVGDEPIVLARRSGCPVWIGPDRVQVAAVLRAAHPDVDVLVLDD
                     GLQHYRLRRDIEIAVVDARGLGNGFMLPAGPLREPRLRLRTVNAVVAHGDSPVKDFSM
                     SLAGEEVHRMTDARERRSLKAFAGQKVHGAAGIGDPNRFFVHLGRAGVKVIPHPFPDH
                     HPFTARDLDFGDGLPVILTEKDAVKLRSAARPEWWVLPVSARLDPGFGAWLVRKLDEC
                     RRSKIA"
BASE COUNT          692 a         1420 c         1386 g          679 t
ORIGIN      
        1 tcctcgatgt acttcttggc gttgtcccag gcgccgccgc cggtgcacat cgagatcgcg
       61 acgaagaggc cggtgacgat ggtgcccatc agcacgccgc ccagcgcctg cggcccgagg
      121 agcaggccga ccaggatcgg caccagcacc ggcagaagcg aggggatcat catttcgcgg
      181 atggctgcgc gcgtcagcat gtcgaccgcg acgccgtact gcggcttggc cgtcccctgc
      241 atgatccccg gaatctcctt gaactgccgg cgtacttcga gcaccaccgc gccggcggcg
      301 cgcccgacgg cttccatcgc catcgcgccg aagaggtacg gcaccatgcc gccgatgaag
      361 agaccgatga tcaccatgtg gttcgagaga tcgaagctga gggtcttgcc cgccgcctcc
      421 agggcgtgcg tgtaatcggc gaacagcacg agcgccgcga gtcccgccga gccgatggca
      481 tagcccttgg tcaccgcctt ggtggtgttg ccgactgcgt cgagcggatc ggtgatcgcg
      541 cgcaccgagt cgggcaggtc ggccatctcg gcgatgccgc ccgcgttgtc ggtaatcgga
      601 ccatacgcgt cgagcgcgac gatgatgccg gccatcgaca gcatcgaagt ggcggcagtg
      661 gcgatgccgt acaggccggc gagagagtac gaaatgagga tcgcgacgca caccgcgatc
      721 accggccacg cggtggactt catcgacacc ccgaggccgg cgatgatgtt ggtcgcatgc
      781 ccagtgacgc ttgcttcggc gacatggcgc accggcttga actgcgtgcc ggtgtagtac
      841 tcggtgatcc acacgaggaa gccggtgagc gcgaggccga ccaccgccgc gccccacagg
      901 tgccataccg taatgagctt tttcgcgccg ccgatctcga acggcacgtt gcgcagcacg
      961 tcgcccatca cgtagagcgt aatcggccag aacgcgacga gcgcgaggcc gcccgcgacc
     1021 cagaggccgc ggtacagcgc cggcatcacg ttgacctcgc cgggcttggc gcgcacgaac
     1081 caggtgccgg cgatcgaggc gatgatcgcg aagccgccga gcgcgagcgg atagaccagc
     1141 gccgccgggc tctgggtctg caccatcaga gcgccgagca gcatcgtggc gatgatcgtc
     1201 accgcgtagg tttcgaacag atccgccgcc atgccggcgc agtcgccgac gttgtcgccg
     1261 acgttgtcgg cgatcaccgc cggattgcgc gggtcgtcct cgggaattcc cgcctcgacc
     1321 ttgccgacga ggtcggcgcc gacgtcggcg cccttggtga agatgccgcc gccgagacgc
     1381 gcgaagatcg agatcagcga gccgccgaac gcgaggccga cgagcggatg gatgagctgg
     1441 gcgaggttcg ccttgtcgtg cgcattggcg tagagcagcg cgtagtaccc tgccacgcca
     1501 agcagcccga gcccgaccac caggagcccg gtaaccgcgc cgccgcggaa ggcgacctgc
     1561 agcgcctcgt tcagtccgac gcgcgcggcc tcggcggtgc gcacgttggc gcgcaccgag
     1621 atgttcatgc cgatgatgcc cgaggcgccg gagagcaccg caccgaggag aaagccccac
     1681 gccgtcgccc agccgagttg cggcacgaaa ccgatgacca ggaacaggat cacgccgacg
     1741 atcgcgatcg tcttgtactg gcgccaaagg taggcgatcg cgccctcgcg caccgcggcc
     1801 gcgatctcgc gcatgcgctc gttgccgtcg ggcaggcgca ggatgcgcgt agcctgccag
     1861 gcgccataga cgatcgcgag taccgcgcag acgagcgcga agatgagacc tgccgccatg
     1921 tgcttctccc tgagtttgcc cctacgaagg ggcaaaattt tatcagtatc gggtcaactg
     1981 agcgctgcga acgcgcgctt cgtgacctcg tccaggctgc cgcgccccga gatctttcga
     2041 tacttgtccg gcgcgagacg ctggtagtag tcgacgagcg gcctggtctg cgtgcggtag
     2101 atctcgaggc gccttttcac cgtttcctcg cggtcgtcgt cgcgcagcac gagcggctcg
     2161 ccggtcacgt cgtccctccc cttggtcttc ggcggattga acttgacgtg gtagacgcgg
     2221 cccgatccgg gatgcacgcg ccggccgctc atgcgggtaa tgatctcgtc gtccggtaca
     2281 tcgatctcca gcacatagtc gatcgccacg cccgcctcgc ccatctcctc ggcctgccgg
     2341 atggtgcgcg gaaagccgtc gaacaggtag cccgccctgc agtcgggcgc cttcaggcgc
     2401 tccttcacca ggtcgatgat gatgtcgtcc gaaaccagct ggccggcatc cagcaccttc
     2461 ttgacctctg ttccgagcgg cgtacccgct ttcaccgccg cgcgcagcat gtcgccggtg
     2521 gagatctgcg gaatgccgaa cttctcgcgg atgaacttcg cctgcgtgcc cttgcccgcg
     2581 cccggcggtc cgagaaggat caacctcatt aaatgattct acgcacggct tcgagatcct
     2641 gaggcgtgtc gacgcccggc ggaatctcgg tttcgctgac ggcgaccgcg atgcggtggc
     2701 cgtgccacag cacgcgcaac tgctcgaggg cctcggcctt ctccagcggg gaagccttga
     2761 ggcgcgaata cttcttcagg aacgagaccc ggtagccgta gatgccggcg tggcggtagc
     2821 aggtcgcgcc gggctcgcgg ggatggggaa tctgcgccct cgagaagtag agcgcatagc
     2881 cctcggcgtc aagcaccacc ttgaccacgt tcggattggc gagcgaggcc gcatcgtgga
     2941 tcgcgtggca cgcagtcgac acgcttgcgc gcggcttcct ctcgaggatg cgcgccacct
     3001 gcgcgatcag cctgggcgcg atcagcggct cgtcgccctg gacattgacc acgatggatg
     3061 aatccttgag acggagtttt ctcgcggctt cggcaaggcg gtcggtgccc gaggcatggt
     3121 cggctcgcgt catgatcgcc tggtggccgt gcgcgcgtac ggcagcggcg atgcgctggt
     3181 cgtcggtcgc gacgtgcacc gcggccgcac cgctcctggc agcgcgctcg caaacgtgga
     3241 ccaccatggg cttgccgccg atctcggcga gaggctttcc ggggaaacgg gtcgacgcgt
     3301 acctcgccgg gatgatgacc tgaaatctca actgacttct tcttcgggag gcagcttcct
     3361 cgcttcttct tcgagcatca cggggatgcc gtccttcacc ggatagccga ggcggtcggc
     3421 cttgcagacg agctcctgcg cgtgctgagg cgcatttttc ctgtagatga gcttgccctt
     3481 gcacaggggg cagaccagga tttcaagcaa tcttggatcg acggcactca tcgagttttc
     3541 tcacgagcca ggcgccgaac ccgggatcga gccttgcgct caccgggagc acccaccact
     3601 ccggccgcgc ggcgctgcgc aattttaccg cgtccttctc ggtgaggatc acgggcagtc
     3661 cgtcgccgaa gtcgaggtcg cgcgccgtga agggatgatg gtcgggaaac ggatgcggga
     3721 tcaccttcac gcccgcgcgc ccgagatgca cgaagaaccg gttcgggtcg ccgatgccgg
     3781 ccgcgccgtg caccttctgc ccggcgaacg ccttgagcga gcgacgctcg cgcgcgtcgg
     3841 tcatccggtg tacctcctcg ccggcaagcg acatcgagaa gtctttcacc ggagagtcgc
     3901 cgtgcgccac caccgcattc acggtcctca ggcgcaggcg cggctcgcgc agcggcccgg
     3961 cggggagcat gaacccgttg cccaggccgc gcgcgtcgac caccgcgatc tcgatgtcgc
     4021 gccgcaggcg gtaatgctgc aggccgtcgt ccagcaccag cacgtccacg tcgggatgcg
     4081 ctgcgcgcag cacggccgcg acctgcacgc ggtccggccc gatccacacc gggcagccgc
     4141 tgcgccgcgc gagcacgatc ggctcgtcgc cgacctc
//
LOCUS       DATBSZ010000281         2607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1559698, whole genome shotgun sequence.
ACCESSION   DATBSZ010000281 DATBSZ010000000
VERSION     DATBSZ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559698"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..563)
                     /locus_tag="VIG70_18200"
     CDS             complement(153..563)
                     /locus_tag="VIG70_18200"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEY8252530.1"
                     /translation="MDLVGELVVERRGFQHEAILARLLCCGQTQLAEGQAITVIENAD
                     CYGYSLRDVRLFRVTAPAYAVPMVLRLNHNDFLRLRGARGVAIEVMDGRVWITEDGFR
                     ADSFLGAGACYRICGDGLVLVGAEKDDTDIRVLA"
     gene            516..1940
                     /locus_tag="VIG70_18205"
     CDS             516..1940
                     /locus_tag="VIG70_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY8252531.1"
                     /translation="MLETTPLYHQLADQIHELIRAGTLRAGDRVPSVRRLSSQQRVSV
                     STVLQAYQRLEDIGAIEARPQSGYYVRRLNAAVQEPEPSQPPKRALHVEVNELTDAVL
                     TYAADPNVVAFGSACAAPELFQLERVRRVLSSLARRDRNALGRYGLPPGTEKLRRAVA
                     RRALEWGCRIDHRHLVTTTGCIEAINLALRAVTKPGDLVALESPTFYGFLQILQSLGL
                     RALEIPTHPRSGISLEALELALAEHPVKAVLVMPSVSNPIGASMSDAAKKRLVELLAA
                     KGVPLIEDHIYAELQFGSTPPRPAKAFDRSGNVMLCSSFSKTLAPGLKVGWIEPGRWR
                     DSVRMLKFMSSGGQNELVELAVAELLESGGYERSLRQLRRRFESQVDLARGVIAESFP
                     RGTRVTRPAGAYIVWVELPKGCDTVLLFERLLERRITIAPGPMFSASQRYRNCMRLSV
                     GQAWADKHERALREVGRLAQELFG"
     gene            1958..>2607
                     /locus_tag="VIG70_18210"
     CDS             1958..>2607
                     /locus_tag="VIG70_18210"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY8252532.1"
                     /translation="MPLVDIGANLTHPAFAPDLDEVLARARQAGVETVIVTGTTVEES
                     RQASTLGLPFTAGVHPHHARDCGPDTIVQLKTLAVDPHCVAIGECGLDFNRNYSPHPD
                     QEKWFIAQLELGISLGRPLFLHSRDAHPRFSEILKAHAVKKAVAHCFTGEREELRAYL
                     DLGLYVGITGWICDERRGKHLVELVREIPRNRLLLETDAPYLTPRDLRPQPKARRN"
BASE COUNT          428 a          941 c          839 g          399 t
ORIGIN      
        1 ggcgctcgag gccgcccgcg acaagctgct gaaggacgag ctagggcgcc gccacctcgc
       61 ctcgcccatc cgcttccgcg atgagcccgc ccgcccgaac ctccgcgagc cgcggctcgg
      121 cgagcacgac gcgctgaaga agccctgagc tcctacgcaa gaacccgaat atccgtgtca
      181 tctttttccg cgccgaccag aaccaggccg tcgccgcaaa tccggtagca agcgcccgcg
      241 ccgaggaagc tgtcggcccg gaagccgtcc tcggtgatcc agacgcggcc atccatgacc
      301 tcgatcgcca cgccgcgcgc gccgcgcagc cgcaggaaat cgttgtggtt caatcgcagc
      361 accatgggga cagcgtaggc cggagccgta acccggaaca gcctcacgtc ccgtaaactg
      421 tacccgtaac aatccgcgtt ctcgatcact gttatggcct gcccttcggc caactgtgtc
      481 tggccgcagc acagcagcct tgcgaggatg gcttcatgct ggaaaccacg ccgctctacc
      541 accagctcgc cgaccagatc cacgagctga tccgtgccgg cacgctgcgc gccggcgacc
      601 gcgtgccctc ggtgcggcgg ctttccagcc agcagcgcgt aagcgtctcg acggttctgc
      661 aggcctacca gcggctcgag gacatcggcg caatcgaggc gcgcccgcag tcgggctatt
      721 acgtgcggcg cctcaacgcg gcagtgcagg agcccgagcc gtcgcagccg ccgaagcgcg
      781 cgctgcacgt cgaggtcaac gagctcaccg acgcggtgct cacctatgcc gccgacccga
      841 acgtggtggc gttcggctcg gcttgcgcag cgccggagct gttccagctc gagcgcgtgc
      901 gccgggtcct atcctcgctc gcgcgccgcg accgcaacgc gctcggccgc tacggcctgc
      961 cgccgggcac cgagaagctg cggcgcgcgg tcgcgcgccg cgcgctcgaa tggggctgcc
     1021 gcatcgacca tcgccacctc gtcacgacga ccggctgcat cgaggcgatc aacctcgccc
     1081 tgcgcgcggt caccaagccg ggcgacctgg tggcgctgga gtcgccgacc ttctacggtt
     1141 tcctccagat cctgcagagc ctcggcctgc gcgcgctcga gatccccacc catccacgca
     1201 gcggcatctc gctcgaggcg ctcgagctgg cgctcgccga gcacccggtg aaggcggtgc
     1261 tggtgatgcc cagcgtgtcc aatccgatcg gcgcctcgat gtccgacgcg gcgaagaagc
     1321 gcctggtgga gctgctggcg gcgaagggcg tgccgctgat cgaggaccac atctacgccg
     1381 agctgcagtt cggctccacg ccgccgcggc cggcgaaggc cttcgaccgc agcggcaacg
     1441 tgatgttgtg cagctcgttt tcgaagacgc tggcccccgg gctcaaggtc ggctggatcg
     1501 agcccggccg ctggcgcgac agcgtgcgga tgctgaaatt catgtcgagc ggcgggcaga
     1561 acgagctggt cgagctcgcg gtggccgagc tgctggaatc cggcggctac gagcgctcgc
     1621 tgcgccagct gcggcggcgc ttcgagtcgc aagtcgacct cgcgcgcggc gtcatcgccg
     1681 agtcgttccc gcgcggcacg cgcgtgacgc ggcctgccgg cgcctacatc gtctgggtcg
     1741 agctgccgaa aggctgcgac acagtgcttt tgttcgagcg cctcctcgag cgccgcatca
     1801 ctatcgcgcc gggcccgatg ttctcggcct cgcagcgcta ccgaaactgc atgcgcctga
     1861 gcgtcggcca ggcctgggcg gacaagcacg agcgcgcgct gcgcgaagtc gggcgcctcg
     1921 cgcaggagct cttcggctag aaagctatac tcggccgatg ccgctggtcg acatcggcgc
     1981 aaacctgacg cacccagcct tcgctcctga cctcgacgaa gtactggcgc gcgcgcggca
     2041 ggccggcgtc gagacggtca tcgtcaccgg cactaccgtc gaggagagcc ggcaagcgtc
     2101 gacgctcggc ttgcccttta ccgcgggagt gcatccgcat cacgctcgcg actgcggacc
     2161 agatacgatt gttcaactca agacccttgc cgtggatccg cattgcgtcg caatcggtga
     2221 atgcggcctg gacttcaacc gcaactactc gccgcatccg gaccaggaga aatggttcat
     2281 cgcgcagctc gagctcggca tctcgctcgg caggccgctc ttcctgcatt cgcgggacgc
     2341 gcatccccgg ttttccgaga ttctgaaagc ccacgccgtg aagaaagccg tggcgcattg
     2401 cttcaccgga gaacgagagg aactccgcgc gtatctcgat ctcggcctct acgtcggcat
     2461 caccggctgg atctgcgacg agcgccgcgg caagcatctc gtcgagctgg tgagggaaat
     2521 tccccgcaac cggctgctgc tggaaaccga cgcgccctat ctcacgccgc gcgatctcag
     2581 gccgcaaccg aaggcgcggc gcaacga
//
LOCUS       DATBSZ010000282        12510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1559715, whole genome shotgun sequence.
ACCESSION   DATBSZ010000282 DATBSZ010000000
VERSION     DATBSZ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12510
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559715"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /gene="dnaG"
                     /locus_tag="VIG70_18215"
     CDS             complement(<1..1237)
                     /gene="dnaG"
                     /locus_tag="VIG70_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY8252533.1"
                     /translation="MIPDSFKQDLLNRVDIVDVVQRYVQLRKAGTNYVGLCPFHNEKT
                     PSFSVSPTKQFYHCFGCGVHGNAIGFLMAYASLGYVDAVKDLASQAGMQVPESRPRSP
                     EEAARQEREPDLYALMEKAMDFYRAELKKSPRAIEYLKGRGLTGEIAARYRIGYAPDD
                     WQALQGSFSDYQDKSLVEVGLVIQNEEGKRYDRFRDRVMFPILNARGAVIGFGGRILD
                     KGEPKYMNSPETPLFEKGRELYGLPQARDAIRTAGRVLVVEGYMDVVALAQFGVGYAV
                     ATLGTATTPVHVSKLLRLADALVFSFDGDAAGRKAAWRALEVSLPLAPDHKPIRFLFL
                     PEGEDPDTYIRKHGREAFETRVREAETLSQFLLAQLRAECDLGTAEGRARFLSMAKPH
                     VQKITAPALRLQLINEIAQL"
     gene            complement(1260..1706)
                     /locus_tag="VIG70_18220"
     CDS             complement(1260..1706)
                     /locus_tag="VIG70_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY8252534.1"
                     /translation="MTLKAKITEDMKSAMRAKDSARLSAVRLILAAIKQREVDERKEL
                     SDAEVTSVIEKMIKQRRESIAQFEQASRKDLADAEKFELEVLSAYLPKQMNDSEVSQA
                     VDAAISEAKPSGVKDMGKVMALLKPRLAGRADMGKVSALVKTRLSG"
     gene            complement(1703..1915)
                     /gene="rpsU"
                     /locus_tag="VIG70_18225"
     CDS             complement(1703..1915)
                     /gene="rpsU"
                     /locus_tag="VIG70_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY8252535.1"
                     /translation="MPTVRVKENEPFEVAMRRFKRTVEKTGLLTELRSREYYEKPTAE
                     RKRKLAAAVKRHHKRLRSQMLPQKLY"
     gene            1960..2940
                     /gene="tsaD"
                     /locus_tag="VIG70_18230"
     CDS             1960..2940
                     /gene="tsaD"
                     /locus_tag="VIG70_18230"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY8252536.1"
                     /translation="MLILGIETSCDDTGVALYDSRAGKLVAHEVHSQAAMHQAYGGVV
                     PELASRDHIRRLVPLTRKVVQKRTIDGVGYTAGPGLAGALLVGASFAHGLALSLGVPA
                     VAIHHLEGHLLSPLLSATRPDFPFLALLVSGGHTQLMRVDGVGRYQLLGDTQDDAAGE
                     AFDKTAQLLGLGYPGGPALSKLAERGAPGRYRLPRPMIGAGLDFSFSGLKTAVLTLVK
                     RGDADPADLARAFVDAIVEVLVAKCSRAAETTGLTRLVVAGGVGANRQLRAALDAEGA
                     RRGIDVFYPEPELCTDNGAMIAFAAALRLEGKLPAQEHRFTVKPRWDLTG"
     gene            complement(2906..3517)
                     /gene="plsY"
                     /locus_tag="VIG70_18235"
     CDS             complement(2906..3517)
                     /gene="plsY"
                     /locus_tag="VIG70_18235"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY8252537.1"
                     /translation="MQTAAAIFAAYCLGSISFAIVVSWLLRLPDPRSYGSKNPGATNV
                     LRTGRRAAAVLTLAGDAGKGWAAVWLASFFTTEVALAGLAAFLGHLFPVYHRFRGGKG
                     VATAAGVLFGLDWRVGLGAVVTWLVIVACLRYSSLASLIAALFAPFAAALLLGWQTAL
                     IVGAMSALLFWRHRENIARLVAGTEPRLGEKKPTPSDPSAASP"
     gene            3549..3905
                     /locus_tag="VIG70_18240"
     CDS             3549..3905
                     /locus_tag="VIG70_18240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY8252538.1"
                     /translation="MDAIVIRDLRVEVSIGIHRRERHVLQTVSIDLEIGVPGTTVFTT
                     DKVSDTIDYEQVALGIRALAASGHFRLVETFADRIAALLMNEFGAPWVKVSAAKLGIL
                     PNAKFVGVTIERRRPQ"
     gene            complement(3877..4341)
                     /gene="ybaK"
                     /locus_tag="VIG70_18245"
     CDS             complement(3877..4341)
                     /gene="ybaK"
                     /locus_tag="VIG70_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEY8252539.1"
                     /translation="MTPATAYLAARGIAFSEHTYEYIEHGGTEVPARSLGVPEHHIVK
                     TLVMEDEARKPLIVLMHGDRKVSTKNLARQAGRKRIEPCTPEAAQRHTGYQVGGTSPF
                     GTRKSLPVFVERSILELAEIYINGGRRGLLVKLRTADLAKALRFTAVDVALS"
     gene            complement(4338..5234)
                     /gene="xerD"
                     /locus_tag="VIG70_18250"
     CDS             complement(4338..5234)
                     /gene="xerD"
                     /locus_tag="VIG70_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY8252540.1"
                     /translation="MAANARSARVRAEALNQGLLDAFCDALWLEDGLSRNTIASYRAD
                     LEQFSSFLKGKDLLDVGEENLFAFLGSRKGRASSAARRVSTLKRFYRYCLRERRIDAD
                     PTLKLDPPKRTPRFPKSLSEADVEALLAAADPATPLGLRDRAMLETLYATGLRVSELV
                     TLKTFEVNLDANVIRVVGKGSKERLVPLGEEAAHWISQYLKTRNSKSAALFVTARRAG
                     MTRQAFWHLIRRYGARAIPGKKLSPHVLRHAFATHLINHGADLRVVQLLLGHADISTT
                     QIYTHVARERLKALHAKHHPRG"
     gene            complement(5189..5677)
                     /locus_tag="VIG70_18255"
     CDS             complement(5189..5677)
                     /locus_tag="VIG70_18255"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY8252541.1"
                     /translation="MFDVVLEFPKMKVAVATRDENVVEIRFLPVSSVVVAPRSALAER
                     AARQLERYRDDPDAKFDLPLQVEGSPLQRRVWNAMCAIPRGRTRTYGELARELGAHFP
                     VDARAIGQACGDNRLPIVIPCHRIVAADGLGGFGHSTGGYLLEVKRWLLMHEAPAFEL
                     RP"
     gene            complement(5665..6666)
                     /locus_tag="VIG70_18260"
     CDS             complement(5665..6666)
                     /locus_tag="VIG70_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY8252542.1"
                     /translation="MKKDAQELHRLARTALEKAGANSKMAEAAARHLVRAEEQGLPTH
                     GMSRVPFYCGMLRKGRADGAAQPAMVADRAAVCLIDNRDGLPYVSVQWAVEEVIQRAR
                     RNGIGFAGIRNSAHVGVLGIHLLAIAEAGLVGLAFTNSPAAIPAWGGKKALFGTNPVA
                     GVFPRKHKDPIVVDLALTTVVRGRIMMAMRKGERIPEGWALDRHGRPTTDPKEAIEHG
                     SLFPIGGAKGAMLGLMFELICASLTGAAIGPEADSFFSEEGNRPRIGQAFIAIDPGAL
                     AGMDKFSERVETVVKTMLADPEVRLPGARRFASEASSRKGIEVPDELLAQIEKLCST"
     gene            6686..7354
                     /locus_tag="VIG70_18265"
     CDS             6686..7354
                     /locus_tag="VIG70_18265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8252543.1"
                     /translation="MGKIIELTAADGHKLAAYRAEPSGKPRGGIVVIQEIFGVNSHVR
                     SLADGFAADGYLAIAPAMFDRAQKNFEVGYTPPDIEKGRELRAKITVDMAMKDAQAAV
                     DEAKKAGKVGIVGYCWGGFVAWMASAKLSGLSAAVPYYGGGMLDNTDIEPKVPVMAHF
                     GEKDSMIPVAGVKKLAEKHKKHQVFIYAADHGFNCDQRGSYDAPSAKQARTRTLEFFR
                     KHVG"
     gene            complement(7325..7918)
                     /locus_tag="VIG70_18270"
     CDS             complement(7325..7918)
                     /locus_tag="VIG70_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY8252544.1"
                     /translation="MKAALHRREVVKSLLKDRKDLLVVAGLGSTSWDITAAGDSPLSF
                     PTWGAMGQAAMMGLGLALAQPRRRVLVVTGDGEMLMGLGALATIGVQRPANLSIVVID
                     NERYGETGMQTTHTAHEVDLAAVARACGFKKSTVIGNSAGVAKLRAVIHGKSGPNFAQ
                     VKVVAEKLPLVLPPHDGVVLKERFRAALLADVLPEKL"
     gene            complement(7915..8424)
                     /locus_tag="VIG70_18275"
     CDS             complement(7915..8424)
                     /locus_tag="VIG70_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY8252545.1"
                     /translation="MKWPEDVHRGLSAAGVRVVGYVPDAGHKRLIELCQADRKMRSVV
                     LTTEEEGIGLAAGAWLGGRKSVLLMQSSGVGNIVNVLGMVQVCRFPLVMIVTMRGEQG
                     EFNPWQVPMGLATPQVLEAMGVSVRRASDAAEVAPLVAEAFKGETAAAVLIAQKVIGI
                     KSFQEQRAQ"
     gene            complement(8411..10141)
                     /locus_tag="VIG70_18280"
     CDS             complement(8411..10141)
                     /locus_tag="VIG70_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY8252546.1"
                     /translation="MRRLDSDILILGSGGAGLFAALHAHQAQMRAASPLSITVAVKGL
                     LGKCGCTRMVQGGYNVALAREDSVERHFMDTIVGGAWLSDQELAWKLVTLAVERIREL
                     ENELGCFFDRNPDGTIHQKAFAGQTFDRTVHKGDLTGIEIINRLAEQVWARKIARLEE
                     HRAVELIKAGDGKSISGVLFIDMRTGEYVFVRAKAVLLATGGGPTMYKYHTPSGDKTC
                     DGLAMALRAGLPLRDMEMVQFHPTGLLAGTHTRMTGTVLEEGLRGAGGYLLNGSKERF
                     MPGYDPKAERATRDRVSRAMFSEIRKGDTTPHGGLYLSMAHLGPQKVAKDFKGMVERC
                     ADCGFDLAGGLVEVVPTAHYMMGGVEFAPDCTTELPGLFVAGEDSGGVHGANRLGGNG
                     VANSTVFGAVAGDAMAAWVKDAALREPERAAIDVAVAACERPFRNSGKGNLEALREKL
                     YDVMWDKVGIVRDAAGLLQAQADLNALDRALDAHSIPGAERAFNLTWHDWLNLKNLVA
                     VSRAIAVAAEARRDSRGAHWRSDFPEPGPLERSTFTSIRNMTVSMKPVAFTRVAPGQT
                     LLQGVENEVA"
     gene            complement(10125..12170)
                     /locus_tag="VIG70_18285"
     CDS             complement(10125..12170)
                     /locus_tag="VIG70_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEY8252547.1"
                     /translation="MSEEAVRIHQPAAEVSKEALARAEEMIEREEGAQNKLAGWLAAL
                     VATVAVAMSVFHLYTAYAIVRPEHLRATHVAFVLFLTFLIFPVARRFRHRVMWWDWAL
                     AAAGVGTAVYLIAGGDEFFDRSILPDDWDIAFGVLLVVLVLEAMRRTTGWILPVITAC
                     FLAYAFFGPYLPAPWTHKGYEVPRLVGHMYMTLEGIYGTAINVSSTLIILFTIYGAFL
                     QYSGAGKFFIDFSFSAMGGKPTSAGRTVTFASFLLGGPSGSGVATTVTLGAVAAPMLA
                     KVGYSREEAGGLLAAGGLGAIISPPVLGAAAFIIAEFLKISYLDVVLMAVIPTCLFYL
                     TLFLMVEIDVRKFGMRNVTFEAVESVWSLAKRYWFHFLSLVSIVAFMLVGFSPELSVF
                     WATVVAFATSFLRRDTALISFDVLRGREPVLAGLVNSGFSKALQGGSMSVLNVAATCA
                     GAGIIVGVVTLTGLDLKFSSIVLAYAGNSLLLTAIYTALIVWIVGLAVPVTASYIICA
                     VIAAPALIALKVPEFAAHMFIFYYAVLSEVSPPTALSPFAAAAITGGDPYKTTLQCWK
                     YTTPAFLVPFMFVLDPSGAGLLLTGSFKTLGDANWGSIALVTFTAAVGIMALCGGFQG
                     WLFKKTTVYERLMLLVAGVLLVYPKTLFDAVGFGLVAFVLASQFLVKRAEPRRASAR"
     gene            complement(12232..>12510)
                     /locus_tag="VIG70_18290"
     CDS             complement(12232..>12510)
                     /locus_tag="VIG70_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY8252548.1"
                     /translation="VQDRIPKETYRGMDADNRQATVMNLLVAHENMDEKTAYNIVKAV
                     FDHRDELIRVHKEAENFKLENQKTAAAGGIPWHPGAVRYFKEKSVKLD"
BASE COUNT         2091 a         4250 c         4112 g         2057 t
ORIGIN      
        1 cgagctgcgc gatctcgttg atcagctgca ggcgcagcgc cggcgcggtg atcttctgaa
       61 cgtgcggctt cgccatggaa aggaagcgcg cacgcccctc cgccgttccg aggtcgcatt
      121 ccgcgcgcaa ttgcgcgagc aggaactggg aaagcgtctc ggcctcgcgc acgcgcgtct
      181 cgaacgcttc ccggccgtgc ttgcggatgt aggtgtccgg gtcctcgcct tcgggcagga
      241 agaggaagcg gatcggcttg tggtcgggcg cgagcggcag gctcacctcg agggcgcgcc
      301 acgccgcctt gcgccccgcg gcatcgccgt cgaagctgaa cacgagcgcg tcggcgaggc
      361 gcagcagctt cgaaacatgc accggggtcg tagccgtgcc gagcgtggcc accgcgtagc
      421 cgacgccgaa ctgcgcgagc gccacaacgt ccatgtatcc ctcgactacc aggacccgcc
      481 cggccgtccg gatcgcgtcg cgcgcctgcg gcaacccgta gagctcgcgt cctttctcga
      541 acagcggcgt ctccggggag ttcatgtact tcggctcgcc cttgtcgagg atgcggccgc
      601 cgaagccgat caccgcgccg cgcgcattca ggatcgggaa catcacccgg tcgcgaaagc
      661 ggtcgtagcg cttgccctcc tcgttctgga tcacgaggcc gacctcgacc aggctcttgt
      721 cttggtaatc tgaaaacgaa ccctgcaatg cctgccagtc gtcaggcgca tagccgatgc
      781 gataacgggc cgcgatctcg ccagtgaggc cgcgcccctt caggtactcg atcgcgcgcg
      841 gcgatttctt gagctcggcg cggtagaagt ccatcgcctt ttccatcagc gcgtagaggt
      901 cgggctcgcg ctcctggcgc gcggcctctt ccgggctgcg cgggcgcgat tcgggcacct
      961 gcatcccggc ctgcgaggcg aggtccttca ccgcgtcgac atagcctagc gacgcgtacg
     1021 ccatgagaaa tccgatcgcg ttgccgtgca cgccgcaccc gaagcagtgg tagaactgct
     1081 tcgtcgggct cacgctgaac gacggggtct tttcgttgtg gaacgggcac agcccgacgt
     1141 agttcgtgcc cgccttgcgc aactggacgt agcgttgcac tacgtcgacg atgtctaccc
     1201 gattgagcag gtcctgcttg aacgaatcgg gaatcacgct cccgattctc gcaaattttt
     1261 tatcccgaga gccgggtttt gacgagcgcg gagaccttcc ccatgtcggc gcggcccgcg
     1321 agccgcggct tgagcagcgc catcaccttg cccatgtcct tgacgcccga gggtttcgcc
     1381 tcggagatcg cggcatcgac ggcttgcgag acttcgctgt cgttcatttg cttgggcagg
     1441 taggctgaca gcacctcgag ctcgaatttc tccgcgtccg ccaagtcctt gcgcgacgcc
     1501 tgctcgaact gggcgatcga ctcgcggcgc tgcttgatca tcttctcgat caccgaggtc
     1561 acttcggcgt cgctgagctc cttgcgctcg tccacttccc gttgcttgat cgcggccagg
     1621 atcaggcgca cggcggacag gcgcgcggaa tccttggcgc gcatcgccga cttcatgtct
     1681 tcggtgattt tggcttttaa ggtcagtaca gcttttgcgg cagcatctgg ctgcgcagac
     1741 gcttgtggtg ccgcttgacg gctgctgcga gcttgcgctt gcgttctgcg gtcggcttct
     1801 cgtagtactc gcgggagcgc agctcggtca gaaggccggt cttctccacg gtgcgcttga
     1861 agcggcgcat cgccacttcg aacggctcgt tttccttgac gcgtacggtc ggcataaacc
     1921 ctggatcgaa aaggggctga attgtatcag aatgacctcg tgctgatcct cggcatcgag
     1981 acctcctgcg acgacaccgg cgtggcgctt tacgacagcc gcgcgggcaa gctcgtcgcg
     2041 catgaggtcc actcccaggc ggcaatgcat caggcctacg gcggggtagt tccggagctc
     2101 gcctcgcgcg accacatccg caggctggta ccgcttacgc gaaaagtggt ccagaaaaga
     2161 accatcgacg gggtcggtta caccgctggg ccggggctcg ccggcgcttt gttagtgggc
     2221 gcttctttcg ctcatggcct cgccttgtcg ctgggggtgc ccgcggtggc gatccatcac
     2281 ctcgaaggcc acctgctgtc gccgctcctc tccgcgacgc gcccggattt tccgttcctt
     2341 gcgctcctcg tttccggcgg ccatacccag ttgatgcgag tcgacggcgt agggcgctat
     2401 cagctcctcg gcgacaccca ggacgatgcc gccggcgagg ccttcgacaa gaccgcgcag
     2461 ctcctcggcc tcggctaccc cggcgggccg gcgctctcga agctggccga gcgcggtgcg
     2521 ccgggccgct atcgcttgcc gcgtcccatg attggcgccg gcctggactt cagcttctcc
     2581 ggcctcaaga ccgcggtgct gacgctcgtc aagcgcgggg acgccgaccc ggccgacctt
     2641 gcccgcgcgt ttgtggacgc tatcgtggag gtgcttgtcg cgaagtgcag ccgcgcagcg
     2701 gagaccaccg ggcttacccg gctcgtcgtc gccggcggcg tcggcgccaa ccggcagctg
     2761 cgcgcagcgc tcgacgccga gggcgcccgg cgaggcattg atgtgttcta tcccgagccg
     2821 gagctctgca ccgacaacgg ggcgatgatt gcctttgcag cggccctcag gctcgaaggc
     2881 aagttacccg ctcaagagca tcgattcacg gtgaagccgc gctgggatct gacggggtag
     2941 gcttcttctc gccgagacgc ggctcggtgc cggcaacgag gcgcgcgatg ttctcccggt
     3001 gccgccagaa gagaagcgcg gacatcgcgc ccacgatgag cgcggtctgc cagccgagca
     3061 ggagcgcggc ggcaaagggc gcgaacagcg cggcaatcag cgaggcgagc gacgagtagc
     3121 gcaggcacgc gacgatcaca agccaggtga ccaccgcgcc gaggccgacg cgccaatcga
     3181 gcccgaacag cacgcccgcc gccgtcgcca cgcccttgcc tccccgaaag cggtggtata
     3241 ccgggaagag atggccgagg aacgccgcga ggcccgcaag ggcgacttcg gtggtgaaaa
     3301 acgacgccag ccacaccgca gcccagccct tgcccgcgtc gccggccagg gtgagcaccg
     3361 cagcggccct tcggccggtg cgcagcacgt tggtcgcccc gggattcttc gagccgtagc
     3421 tgcgcgggtc gggcaggcgc aggagccagc tcacgacgat cgcaaacgag atcgacccca
     3481 ggcagtacgc agcgaagatc gcggcagcgg tctgcattgc ggggcgattc tatagaatcg
     3541 tgccggacat ggatgcgatc gtcatccgcg acctgcgcgt tgaggtgtcc atcggcatcc
     3601 accggcgcga gcgccacgtg ctgcagacgg tatcgatcga cctcgagatc ggcgtgccgg
     3661 gcaccaccgt gttcacgacc gacaaggtgt ccgacaccat cgactacgag caggtggcgc
     3721 tcggcatccg cgcgctcgcc gcgagcgggc acttccgcct ggtcgagacc ttcgccgacc
     3781 gcatcgccgc gctcctcatg aacgagttcg gcgcgccctg ggtgaaagtg agcgccgcaa
     3841 agctcggcat cctgcccaac gccaagttcg tcggcgtcac gatagagcga cgtcgaccgc
     3901 agtaaagcgc aacgccttcg ccaaatcggc ggtgcggagc ttcaccagca ggccgcggcg
     3961 cccgccgttg atgtagatct cggcgagctc gaggatcgag cgctcgacga aaaccggaag
     4021 gctcttccgc gtgccgaagg gcgaggtgcc gccgacctgg tagccggtgt ggcgctgcgc
     4081 cgcctccggc gtgcacggct cgatgcgctt gcggccggcc tggcgcgcga ggttcttggt
     4141 cgacaccttg cgatccccgt gcatcagaac gatgagcggc ttgcgcgcct cgtcctccat
     4201 caccagcgtc ttcacgatgt gatgctccgg cacgccgagc gatcgggcgg gcacttcggt
     4261 gccgccatgc tcgatgtatt cgtaggtgtg ctcggaaaaa gcgattcccc gagccgccaa
     4321 gtaggccgtc gccggggtca tcctcgcgga tgatgtttcg cgtgcagcgc tttcaggcgt
     4381 tcgcgcgcga cgtgcgtgta gatctgcgtg gtggagatgt ccgcgtgccc gaggagaagc
     4441 tgcaccaccc gcaggtcggc gccgtggttg atgaggtgcg tcgcgaacgc gtggcgcagc
     4501 acgtgcggcg agagcttctt tcccggaatg gcgcgcgcgc cgtagcgccg gatgaggtgc
     4561 cagaacgctt gccgcgtcat gccggcgcgg cgtgcggtca cgaacagggc ggcggacttc
     4621 gagtttcggg ttttcaggta ctgcgagatc cagtgcgcgg cctcctcgcc cagcggcacc
     4681 aggcgctcct tgctgccctt gcccactacg cggatgacgt tggcgtccag gttcacttcg
     4741 aatgtcttga gcgtcacgag ctccgacacc cgcagccccg tcgcgtagag cgtctcgagc
     4801 atcgcccggt cgcgcaggcc cagcggcgtc gccgggtccg cggccgcgag cagcgcctcg
     4861 acatcggcct cggacagcga cttcgggaag cgcggggtgc gcttgggcgg atcgagcttg
     4921 agcgtcgggt ccgcatcgat gcgccgctcg cgcaggcaat accggtagaa gcgcttgagc
     4981 gtggaaacgc gccgcgccgc gctcgaggcg cgtcctttgc gcgagccgag gaaggcaaag
     5041 aggttttctt ccccaacgtc gagcaggtcc ttgcctttca ggaaactgga aaactgctca
     5101 aggtcggcgc ggtacgacgc gatggtattg cgcgagaggc cgtcctccag ccaaagcgcg
     5161 tcgcagaacg cgtccagcag gccctggttc agggcctcag ctcgaacgcg ggcgcttcgt
     5221 gcattagcag ccaacgcttt acctccagca ggtagccgcc ggtcgagtgc ccgaagccgc
     5281 cgagaccgtc cgcggcgacg atgcggtggc agggaatcac gatcggcagg cggttgtcgc
     5341 cgcaggcctg cccgatcgcg cgtgcatcga cgggaaaatg agcccctagc tcgcgcgcca
     5401 gctcaccgta ggtgcgcgtc ctgccgcgcg gaatcgcgca catggcgttc cacaccctac
     5461 gctgcagcgg cgagccctcg acctgcagcg gcagatcgaa tttcgcgtcc ggatcgtcgc
     5521 ggtagcgctc gagctggcgc gcggcacgct ccgcgagcgc gcttcttggg gccacgacaa
     5581 ccgatgacac cggcaagaag cggatctcga cgacattctc gtcgcgcgtc gccacggcga
     5641 ccttcatctt cgggaactcg agaactacgt cgaacatagc ttctcgatct gcgccagcag
     5701 ctcgtccgga acctcgatgc ccttccttga cgaggcctcc gacgcgaagc gccgcgcgcc
     5761 tggcaggcgc acctcggggt cggccagcat ggttttcacc acggtttcca ccctctccga
     5821 gaacttgtcc atgccggcaa gcgcacccgg atcgatcgcg atgaacgcct ggccgatgcg
     5881 cgggcggttg ccctcctccg agaaaaacga atccgcctcg gggccgatcg cagcgccggt
     5941 gagggaggcg cagatgagct cgaacatcaa gccgagcatt gcgcccttcg cgccgccgat
     6001 cggaaacagg ctgccgtgct cgatcgcctc cttcgggtcg gtggtcggcc ggccgtggcg
     6061 gtcgagcgcc cacccttccg ggatgcgctc gcccttgcgc atcgccatca tgatgcgccc
     6121 gcggaccacg gtcgtgaggg cgagatcgac cacgatcggg tccttgtgct tgcgtggaaa
     6181 cactcctgcc accgggttcg tgccgaacag cgccttcttg ccgccccagg cgggaatcgc
     6241 cgccggggaa ttggtgaacg caagaccgac cagcccggcc tcggcgatcg ccagcaggtg
     6301 gatgccgagc acgccgacgt gcgcgctgtt gcggatgccg gcgaagccga tgccgttgcg
     6361 ccgggcgcgc tggatcacct cttcgaccgc ccattgcacc gagacgtagg gcaggccgtc
     6421 gcggttgtcg atcaggcaga ccgccgcccg gtcggcgacc attgccggct gggcggcgcc
     6481 gtcggcgcgg cccttgcgca gcatcccgca atagaaaggc acgcgcgaca tgccgtgcgt
     6541 cggcagcccc tgttcctcgg cgcgaaccag atggcgcgcc gcggcctcgg ccatcttaga
     6601 attcgcccca gccttctcca gggcggtgcg cgccaggcgg tgcagctcct gcgcatcttt
     6661 cttcacaagc gaggattcta cggccatggg aaaaatcatc gaactgacag ccgccgacgg
     6721 ccacaagctg gcggcgtatc gcgcggaacc gtcggggaaa ccccgcggcg gcatcgtcgt
     6781 catccaggag atcttcggcg tcaacagcca cgtgcgctcg ctcgccgacg gcttcgccgc
     6841 cgacggctac ctcgcgatcg cgccggcgat gttcgaccgg gcgcagaaga acttcgaggt
     6901 cggctacacg ccgcccgaca tcgagaaggg gcgagagctg cgcgcgaaga tcaccgtcga
     6961 catggcgatg aaggacgccc aggcggcggt ggacgaggcc aagaaagccg gcaaggtcgg
     7021 catcgtcggc tattgctggg gcggcttcgt cgcctggatg gcctcggcca agctgagcgg
     7081 cctctcggcc gcggttccgt attacggcgg cggcatgctc gacaacaccg acatcgagcc
     7141 gaaggtgccg gtgatggcgc acttcggcga gaaggactcg atgatcccgg tcgccggcgt
     7201 gaagaagctg gccgagaagc acaagaagca ccaggtgttc atctacgccg ccgaccacgg
     7261 cttcaactgc gaccagcgcg gctcttacga cgcgccgtcg gcgaagcagg cgcgcacccg
     7321 gacgctagag tttttccgga agcacgtcgg ctagcagcgc ggcccggaag cgttctttca
     7381 ggacgacgcc gtcgtgcggc ggaagcacga gcggcagctt ttccgcgacg accttgacct
     7441 gtgcgaagtt cgggccgctc ttgccgtgga tgacggcccg gagcttcgcc acgccggcgc
     7501 tgtttcctat tacggttgat ttcttgaatc cacacgcgcg ggccacggcc gccaggtcga
     7561 cctcgtgcgc cgtgtgtgtg gtctgcatcc cggtctcgcc gtagcgctcg ttgtcgatca
     7621 ccacgatcga gagattcgcg ggacgctgca cgccgatcgt ggcgagcgcg cccaggccca
     7681 tcagcatctc gccatcgccg gtgaccacga gcacgcggcg gcggggctgc gcgagcgcga
     7741 ggcccagtcc catcatcgcc gcctgtccca tcgcgcccca ggtgggaaag ctgagcggcg
     7801 aatcgccggc cgccgtgatg tcccacgaag tcgaacccag gccagcgacg accagcaggt
     7861 cttttctgtc cttcaacagg gatttgacga cttcccggcg atgcagcgcg gccttcattg
     7921 cgcgcgctgc tcctggaagc tcttgatgcc gatcaccttc tgagcgatca gcaccgcggc
     7981 agccgtctcg cctttgaacg cttcggcgac gagcggcgcg acttcggcgg cgtccgaggc
     8041 gcggcgcacg ctcacgccca tcgcttcgag cacctgcggc gtggcaagtc ccatcgggac
     8101 ctgccaggga ttgaattcgc cctgctcgcc gcgcatcgtg acgatcatca ccagcgggaa
     8161 gcggcacacc tgcaccatgc cgagcacgtt gacgatgttg ccgacgccgc tcgactgcat
     8221 gaggagcacg ctcttgcggc cgccgagcca ggcgccggct gcgagcccga tgccttcctc
     8281 ctcggtggtg agaacgacgc tgcgcatctt ccggtcggcc tggcacagct cgatcagccg
     8341 cttgtggccg gcgtcgggga cgtagccgac gactcgcaca ccggccgcgg acagtccccg
     8401 gtgaacgtcc tcaggccact tcattttcca ccccctgcag cagcgtctga cccggcgcga
     8461 cgcgggtgaa ggctacgggc ttcatcgaga cggtcatgtt cctgatgctg gtgaacgtcg
     8521 agcgctcgag cggccccggc tccgggaaat cggaacgcca gtgcgcgccc cgagaatctc
     8581 gcctggcctc ggccgcgacg gcgatcgcac ggctcaccgc gaccaggttt ttcaggttca
     8641 gccagtcgtg ccaggtgagg ttgaaggcac gctcggctcc gggaatcgag tgggcatcga
     8701 gggcgcgatc gagcgcgttg aggtcggcct gcgcctggag cagccccgcc gcgtcgcgca
     8761 cgatgccgac cttgtcccac atgacgtcgt agagcttttc gcgcagcgcc tcgaggtttc
     8821 ccttcccgga attgcggaaa ggccgctcgc aagccgcgac cgcgacgtcg atcgccgcgc
     8881 gctcgggctc gcgcaaggcg gcgtccttga cccacgcggc catcgcatct cccgcgaccg
     8941 cgccgaacac cgtggagttg gcgacgccgt tgcccccgag ccggttcgcg ccatgcacgc
     9001 cgcccgaatc ctcgcccgcg acgaacaggc ccggcagctc ggtcgtgcag tcgggcgcga
     9061 actcgacgcc gcccatcatg tagtgcgcgg tcggcaccac ctccaccagg ccgccggcga
     9121 ggtcgaagcc gcagtcggcg cagcgctcga ccatgccctt gaagtccttt gcgaccttct
     9181 gcggaccgag gtgcgccatc gacagataaa ggccgccgtg cggagtggta tcgccttttc
     9241 tgatttctga aaacattgcg cgcgacaccc gatcccgcgt ggcgcgctca gccttcgggt
     9301 catagcccgg catgaagcgc tccttcgacc cgttcagcag gtagccgccg gcgccgcgca
     9361 agccttcctc gagcaccgtg ccggtcatgc gcgtgtgcgt gccggcgagc aggccggtgg
     9421 gatggaactg caccatctcc atgtcgcgca gcggcagccc cgcgcgcagc gccatggcga
     9481 gcccgtcgca ggtcttgtcg cccgagggcg tgtggtactt gtacatcgtc ggcccgccgc
     9541 cggtggcgag cagcaccgcc ttcgctcgca cgaacacgta ctcgccggta cgcatgtcga
     9601 tgaagagcac gccggaaatg cttttcccat ctcctgcttt tatcagctcg acggcccgat
     9661 gctcctccaa acgcgcaatc ttgcgcgccc agacctgctc ggcgaggcgg ttgatgatct
     9721 cgatgccggt caggtcgccc ttgtgcacgg tgcggtcgaa ggtctggccg gcgaaggctt
     9781 tctggtggat ggtgccgtcg gggttgcggt cgaagaagca gccgagctcg ttctcgagct
     9841 cgcggatgcg ttccactgcc agcgtcacga gcttccaggc gagctcctgg tcggagagcc
     9901 acgcgccgcc gacgatcgtg tccatgaaat ggcgctcgac cgaatcctcg cgcgcgagcg
     9961 ccacgttgta gccaccctgc accatgcgcg tgcagccgca cttgccgagc agccccttca
    10021 ccgccacggt gatcgagagc ggtgacgccg ccctcatctg cgcctgatgg gcgtgcagcg
    10081 ccgcgaacag gcccgcaccg ccggagccga ggatcaggat gtcgctatcg agccgacgca
    10141 cggcgaggct ccgcacgctt caccaggaac tgcgaggcca gcacgaaagc gacgaggccg
    10201 aaccccacgg cgtcgaaaag cgtcttcgga tagaccagca ggacgccggc gacgaggagc
    10261 atcaagcgct cgtataccgt ggtcttcttg aacagccacc cctggaaccc gccgcacagc
    10321 gccatgatgc ccaccgcggc ggtaaacgtc acaagcgcga tcgagcccca gttcgcgtcg
    10381 ccgagggtct tgaacgagcc ggtgagcagg agccctgcgc cgctcgggtc gagcacgaac
    10441 atgaacggca cgaggaacgc cggcgtggtg tacttccagc actgcagcgt cgtcttgtag
    10501 ggatcgccgc cggtgatcgc cgccgcggcg aacggcgaca gcgcggtggg cggcgagacc
    10561 tccgacagca cggcgtagta gaagatgaac atgtgcgcgg cgaactcggg caccttgagc
    10621 gcgatcagcg ccggggcggc gatgaccgcg cagatgatgt acgaagcggt gaccggcacc
    10681 gccaggccga cgatccacac gatgagtgcg gtgtagatcg cagtcagcag gagcgagttt
    10741 ccggcgtagg cgagcacgat cgagctgaac ttgaggtcaa ggcctgtcag cgtgacgacg
    10801 ccgacgatga ttccggcgcc ggcgcaggtt gcggcgacgt tcagcaccga catcgagccg
    10861 ccttgcaggg cttttgagaa accggagttg accagcccgg ccagcaccgg ctcgcgtcct
    10921 cttagtacgt caaaagaaat caaagccgtg tcccgccgca ggaagctcgt cgcgaacgcc
    10981 accacggtgg cccagaacac cgagagctcg ggcgagaagc ccaccagcat gaacgcgacg
    11041 atcgagacca gcgacaggaa atgaaaccag tagcgcttcg ccaggctcca cacgctctcg
    11101 acggcttcga aggtcacgtt cctcatgccg aatttgcgca cgtcgatctc gaccatcagg
    11161 aagagcgtca ggtagaagag gcaggtcgga atgaccgcca tcagcacgac atccaggtag
    11221 gagatcttga ggaactcggc gatgatgaat gcggccgccc cgagtaccgg cggcgagatg
    11281 atcgcaccca ggccgccggc ggcgagcagc ccgcctgcct cctcccggct gtagcccacc
    11341 ttggcgagca tcggcgccgc caccgcgccg agggtgacgg tcgtcgccac gcccgagccc
    11401 gacggcccgc cgaggaggaa cgaggcgaac gtcaccgtgc ggcccgcgct ggtgggcttg
    11461 ccgcccatcg ccgaaaacga gaagtcgatg aagaacttgc ccgcgccgga gtactgcagg
    11521 aacgcgccgt agatcgtgaa caggatgatc agcgtggacg acacgttgat cgcggtgccg
    11581 tagatgccct ccagcgtcat gtacatgtgg ccgacgagcc gcggcacctc gtagcccttg
    11641 tgcgtccacg gcgccggcag gtaggggccg aagaacgcgt acgcgaggaa gcacgcagtg
    11701 atgactggca ggatccagcc ggtggttcgt cgcatcgctt ccagcaccag cacgaccagc
    11761 agcacgccga acgcgatgtc ccagtcgtcg ggcaggatcg agcggtcgaa gaattcgtcg
    11821 ccgcccgcga tgagatacac cgcagtgccc acccccgcag ccgcgagcgc ccagtcccac
    11881 cacatgacgc ggtgacggaa gcgccgcgca accgggaaga tgaggaaggt gaggaacagc
    11941 acgaaggcca catgcgtggc gcgcaaatgc tcgggccgca cgatggcgta cgcagtgtag
    12001 aggtggaaca ccgacatcgc caccgccacc gtcgcgacga gcgcggcgag ccagccggcg
    12061 agcttgttct gcgccccctc ctcgcgctcg atcatctcct cggcgcgggc aagcgcttcc
    12121 ttgctcacct cggcggcggg ctgatggatg cgaacagctt cttcgctcat gctcctcccc
    12181 taccaatgaa aaagccccgg cgggcggggc tttctcgagg tcaatccact tctagtcgag
    12241 cttgacgctc ttttccttga agtaacggac tgcgccggga tgccacggaa tgcctcccgc
    12301 cgcggcggtc ttctggttct cgagcttgaa gttctcggct tccttgtgca cgcggatcag
    12361 ctcgtcgcga tggtcgaaca ccgccttgac gatgttgtag gcggtcttct cgtccatgtt
    12421 ctcgtgcgcg accagcaggt tcatcaccgt cgcctgccgg ttgtcggcgt ccattcccct
    12481 gtaagtctcc ttcgggatgc ggtcctggac
//
LOCUS       DATBSZ010000283        21044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_125693, whole genome shotgun sequence.
ACCESSION   DATBSZ010000283 DATBSZ010000000
VERSION     DATBSZ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21044
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125693"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VIG70_18295"
     CDS             complement(<1..701)
                     /locus_tag="VIG70_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8252549.1"
                     /translation="MILYVISVGLSVTMGLMGFVNLAHGVFAMAGGFILSTVMGRLGV
                     PFPLALLTAFAAVALASIVLERTLYSRLYAAGELSQVLFCIGLIFVSMAIARLVYGDR
                     TVPVFLPEYLRGQVHVFGRDFPAYRVFLIAFSACLIAVLWFGVERTRWGAMVRAAVDN
                     RGMAQSIGINTKLLFMATFALGSGLAGLGGALGADILPVQWGYPFEHLVYFLIVVAVG
                     GLGSLRGPFAAAVLIG"
     gene            complement(763..1971)
                     /locus_tag="VIG70_18300"
     CDS             complement(763..1971)
                     /locus_tag="VIG70_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8252550.1"
                     /translation="MRSVKFLLAAAFAATASGVAFSASAQTVKIGFITSYSGLNGNLG
                     PYMERAVRLYIKLHEKELPPGVKIELITRDDTGPNPDKAKQLAQELIVRDKVNLLAGV
                     IFTPNALAIAPLATEAKVPFVIMNAGTAVITTRSPYIARTSFTLWQSSFPLGTWAAKK
                     YKTAYTLVSDFGPGHDAETAFTQAFKAGGGNVVANVRVPLQNPDWTAYMQRVKDAKPD
                     ALMVFIPAGKTATAVMKNFSDLGLAGAGIKLIGPGDITTEEELPNMGDVALGVTTVFH
                     WSAAGDRPANKAFVEAWKKEFGAGETPNFLSVGSWDGTALIFEAIKQQKGKLDPDRTM
                     EIFKGWKNPNSPRGPISIDPETRDIIQPEYLREVRKVGGQLANVEIETLGTAVKDPWK
                     EMQKSAPQKK"
     gene            complement(1968..2819)
                     /locus_tag="VIG70_18305"
     CDS             complement(1968..2819)
                     /locus_tag="VIG70_18305"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8252551.1"
                     /translation="MNAADRQRPPAGELYLDHLAHFVPDLGAAAAVWEKLGFTVTPVS
                     HHAVAGKPAGTSNRCVMLEEGYLELLAPAMDTPHAQRVRALMKRYDGVHLACFGTPDA
                     AAEHARLVAHGFEPEPLVSLERAIDTGARLRFKVVYTPPGKMAEGRVQYCEHLTPEHV
                     WREAFVNPFGLRAVYVVADDPEEVAARWGRFGGLLPRPEDGFVRLDTARGRVVIGTQK
                     TLAKLLGEAPPAPALAGYALSCGDPKAFAARCSKAGLKVDGNVVRLPAALGGTWLLES
                     PAKEEDQ"
     gene            2839..4533
                     /locus_tag="VIG70_18310"
     CDS             2839..4533
                     /locus_tag="VIG70_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY8252552.1"
                     /translation="MNKPVELATLTGAEAVVEMLKAHGVDVVFGLCGDTSLPLYDALA
                     RLGAIKHVLTRDERHAAYMADGYARLSGKVGVCEGPSGGGATYILPGLVEANESSIPV
                     LAINTDVSVSSRGKFTLTELDQRTLMKPLTKWNAVLDRSADIPKVLRKAFESMTTGRA
                     GAAHVALPFDVQNGPVERTDVWADPTLGSFPSRRTAPDPALVELAAKLLRKAKQPLFI
                     CGGGVVLSGAESELLELAEKLSAPVATTISGKGSIDERCAHSVGVVGSNGGTPETRAV
                     VDAADLIVFVGCRAGSVTTERWRHPAAGKARILHIDVDPAVPGTNYKVDVPLIGDARL
                     CLSSLNQSLEGYRREADLSWVDEKKRAKFEKFEALARSDDTPIKPERVVFELRKALDA
                     DAIVVADPGTPCPYFSAYYVVKGSGRRYFSNRAHGALGYALAASIGAHIAKPKVKTVA
                     VMGDGSFGMCVGELETAVRLRLPVTFVVISNAVYGWIKAGQKTGYDARYFSVDFGVTD
                     HARVAEAFGVKSWRVFDPAKLGATLKTAVAHDGPTLVDIVCQPLHEARAPVSEWVA"
     gene            4530..5117
                     /locus_tag="VIG70_18315"
     CDS             4530..5117
                     /locus_tag="VIG70_18315"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252553.1"
                     /translation="MKSHHDMGGEPGAKVEPLEHDYADWERRIDAMGVLLWGIKGTKK
                     LLTVDEHRKAIESLAPEAYDSMAYYEKWVFAFSQCLVQRGVVTSAELARKMQDVMFMR
                     QHHDLGGRPAGKVEPTEHDYAQWERRVDALAVLLGSQKVLTVDERRRAIESLPPEAYD
                     AMSYYERWTMALAQTLIQRGIITTEELAKKMNERS"
     gene            5104..5391
                     /locus_tag="VIG70_18320"
     CDS             5104..5391
                     /locus_tag="VIG70_18320"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEY8252554.1"
                     /translation="MNEARFKPGERVRVMKADPPGHIRTPYYIRGCVGEVERICGAFP
                     NPEELAQMRNGLPAQPLYRVRFLQKNIWPDYAGSASDVLEVEIYQHWLEPA"
     gene            5388..5993
                     /gene="nthA"
                     /locus_tag="VIG70_18325"
     CDS             5388..5993
                     /gene="nthA"
                     /locus_tag="VIG70_18325"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEY8252555.1"
                     /translation="MKEQDQGKYRPLSYFQLMEISLRELLVEKGILTNAQVDAEVENM
                     RLRTPERGARVVARAWVDADFKRRLLESGTKACEELGLDVPALKLLAVENTPQVHNAI
                     VCTLCSCYPRMLLGIPPDWYKSRNYRSRMVREPRAVLAEFGLNLPEKVQIRVHDSTAD
                     MRYIVLPMRPGGTEGWNEERLAALVNRDSMIGVAVPKGPHD"
     gene            6015..6725
                     /gene="pxpB"
                     /locus_tag="VIG70_18330"
     CDS             6015..6725
                     /gene="pxpB"
                     /locus_tag="VIG70_18330"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEY8252556.1"
                     /translation="MAQPRPLGVIPLGDSAAYVEFSATLDLEVNSVVQRLALTILKRN
                     VPWVRDVVPALGGLALHFDPDHAEFPAKPLEEALALVIDSLKQALAAKDNPGSEVELP
                     VCYEPEFAFDLAEVAKKADLSPEEVAKRHEAGEYRVLMMGFAPGHAYMGGLDAKLAVP
                     RRASPRAIVPAGSVAIANEQTVVYPYAISGGWNVIGRTPMVLFDATRTRPSLLWPGDR
                     VRFRAISRAEFLSLQKNS"
     gene            6722..7699
                     /locus_tag="VIG70_18335"
     CDS             6722..7699
                     /locus_tag="VIG70_18335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEY8252557.1"
                     /translation="MSVRVLRPGLLTTVQDHGRHGFQRVGLCPGGAMDPVSLALANAL
                     VGNPPEEAALEITVIGPELLFERDTLVAVCGAEFQGSFPHNRPVLAPAGTRFNVGRAT
                     RGARAYIAVAGGFAVDPVLGSRSTYLPGHFGGFEGRALKHGDVLALRDGSAAKKFSIL
                     KKTRDGTVKWSAPPLTLPDREPILVHVIEGQHFASFDSNSQRAFFDTVWRVAPDSNRM
                     GFRLSGPPLGRPQADEILSGPTCLGSVQVPPSGVPIALMADHQTTGGYPRIAEIASAD
                     VARLAQLAPGGKVHFAKCSLEIAGELRRDARARLESALRGIAWEYGAKK"
     gene            7723..8445
                     /locus_tag="VIG70_18340"
     CDS             7723..8445
                     /locus_tag="VIG70_18340"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEY8252558.1"
                     /translation="MGESYGAWKMGADAEVMPHISSANIACGFHGGDPATIRKTVRLA
                     LDNGVAIGAHPSLPDIQGFGRRPMKISPQDMYDLVVYQAGAVEAFARAAGAKLHHIKC
                     HGALYNMAAGDEGLSEAMVRAAKDLGAMLYVLSKSRNYEIARKAGIPVCGEVFADRGY
                     SDDGSLAPRDKPGGMIEDAAKAVKQALSMIEEGTVISVNGKRVPVAADTMCIHGDQPG
                     AASFARALRQAFSEREISVAAP"
     gene            8360..9532
                     /locus_tag="VIG70_18345"
     CDS             8360..9532
                     /locus_tag="VIG70_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEY8252559.1"
                     /translation="METSRARRRSPGRCGRHFRKEKSLSPRPKLFTPIELGGITLPNR
                     IVVSPMCQYSADDGSMTDWHLVHLGTFACAGVGLFVVEKTNVTRDGRITHGCTGLYSD
                     HNEAAMVRAVQVYRGITRNPIGIQIGHAGRKASSHPPFKGGKPLGPHDTPWQTVGPSP
                     IPFGEGWHTPHELSSSELRSLVDSYVAATERAKRIGFDVVELHSAHGYLLHQFLSPLS
                     NQRKDDYGRERMRFPLEVAHAVREVWPKERALGVRINATDWVDGGWQPQDAVAYAREL
                     KRAGFDFVCVSSGATVPYAKIPVAPGYQVPFSEQVRREAGVATRAVGMIADPAQAEEI
                     VASGKADMVAMARAFLDNPRWVWHAAERFGVELDYPPQYARSRHDLWPGAKLARRS"
     gene            9549..9824
                     /locus_tag="VIG70_18350"
     CDS             9549..9824
                     /locus_tag="VIG70_18350"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8252560.1"
                     /translation="MADEKSFEARLRTEGYPEIRTNQLEPNRHNAEHSHPFDVLALVL
                     EGEITLTTEGRGQTYRAGDEFSMQAGCRHVEDAGPQGVRYLVGRRPR"
     gene            complement(9835..9909)
                     /locus_tag="VIG70_18355"
     tRNA            complement(9835..9909)
                     /locus_tag="VIG70_18355"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9876..9878),aa:Ile,seq:aat)
     gene            complement(9969..10331)
                     /locus_tag="VIG70_18360"
     CDS             complement(9969..10331)
                     /locus_tag="VIG70_18360"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8252561.1"
                     /translation="MILLLAACGDSAPPPKPAAQPAAPAPAPQAAAPAPKAPETKAPE
                     APKPDPNKELAQRVKQALEGDGKVQAAGIDVTAKEGRVSLWGTAATAGERNRAGQIAG
                     KVDGVSSVDNQIKVVKGS"
     gene            complement(10422..11264)
                     /locus_tag="VIG70_18365"
     CDS             complement(10422..11264)
                     /locus_tag="VIG70_18365"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEY8252562.1"
                     /translation="MQTDYVQRFLFEDLDIRGRLVCLTGAWQRMLDGRDYPEDIAALL
                     GHTTALNVLLGANQKGSGRVTLQVQGSGPVKLLVSDCTAELRIRGMASYEKPASSFNE
                     RALLGNGRLSVTLEDVHSGQFYQSLVPLEGETLEQIFEHYLSQSEQTTAFLRLRADGG
                     ALCGLLLEKLPRADLCDPDGWNRVMHLANTLTLDETRDAQPYDLLTRVFPEELMRVFR
                     LYAVEYHCPYDETKVKDMLLGLGRDEVESILAEQGEVVIRNEMCNHEYRFDARAIADL
                     FACH"
     gene            11279..13174
                     /gene="glgB"
                     /locus_tag="VIG70_18370"
     CDS             11279..13174
                     /gene="glgB"
                     /locus_tag="VIG70_18370"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY8252563.1"
                     /translation="MPTVADRPISLTGHDLYLFREGTHSRLYEKLGAHVLGDGTQFAV
                     WAPNAAQVSVIGDFNDWDPRAHPMGSSEAGVWSAKVPEAKQGSVYKFHVVSRHGNHRA
                     DKADPYAFRCETPPRTGSVVWKLDYEWNDAEWMKTRSRANALDAPWSVYELHLGSWRR
                     DPSDPRRLLSCRELAPMLAEYCKRMGFTHVELMPVMEHPFYGSWGYQCTGYFAPSSRY
                     GTPQDFMHLVDVLHQNGIGVILDWVPSHFPWDQHGLGFFDGTHLYEHSDPRQGHHPDW
                     ASAIFNYGRNEVRAFLPSSAHFWLERFHADGLRVDAVASMLYLDYGRQAGEWIPNRHG
                     GRENLDAIDFLRFLNETLYRDFPDIQTIAEESTAWPQVSRPNYVGGLGFGMKWNMGWM
                     HDTLGYFKRDPVYRKHHHDELTFSLWYAFHENFVLPLSHDEVVHGKSSLIGRMPGDAW
                     QQFANLRLLFGTMWAHPGKKLLFMGGEFGQRREWTHEDSLEWHVISQDTRHEGVQRWV
                     ADLNRVYRNEKSLHEKDFSGEGFRWINRGDWEASVLSFLRQSPGSDPVLVVCNFTPLP
                     RVNYRVGVPRGGRWRELLNSDAQYYGGSGMGNQGAVEAQPMPYEELSHSLTLTLPPLA
                     VLFFKPE"
     gene            13215..14993
                     /locus_tag="VIG70_18375"
     CDS             13215..14993
                     /locus_tag="VIG70_18375"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEY8252564.1"
                     /translation="MESVRPQVDGGRYPVKRAVGDEVLVEADVFTDGHDVVVAQLLFK
                     FDQDKEWKTLPMEFRHNDHWAASFRVEKLGRYQYTVRGWTDPFLTWRRDLVKRRDAGQ
                     DLTIDLQIGAGILGAEIPSYEAALDVDAPRPREEWIVTHEKVLEVVVDPVRARFSSWY
                     EMFPRSFGGFRGMIEVLPHVERLGFDVLYLPPVHPIGTTARKGKNNAAKALPGDTGSP
                     WAIGSKEGGHKALHPELGTFEDFHALVGAAEKRGIQIALDIAFQCSPDHPYVKDHPEW
                     FRARPDGTIQYAENPPKKYQDIYPFDFESENWTELWNELKSIFEFWVGHGVKIFRVDN
                     PHTKAFEFWEWAISDLKRKHPELIFLSEAFTRPRVMHKLAKLGFTQSYTYFTWRNTKQ
                     ELAEYFTEVTQDSSREYFRPNFWPNTPDILHETLQHGGRPAFIVRTVLAAMAAANYGM
                     YGPAYELQERTPREPGSEEYLNSEKYEIKAWDLNRSDSLAPLISRLNAIRKQNPALQS
                     DWSLKLHPIDNDHLLAFSKKEGDNEILVVVNLDVHHPQSGFVDFPPCEVHDLLSGGHY
                     AWSSGRNYVELSPHKLPAHVFRVTKK"
     gene            14990..18259
                     /gene="treS"
                     /locus_tag="VIG70_18380"
     CDS             14990..18259
                     /gene="treS"
                     /locus_tag="VIG70_18380"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEY8252565.1"
                     /translation="MNERATPSLVTPPLEAAVETDALWYKDAVIYQLHVKAFFDSNND
                     GMGDFGGLMQKLDYIQELGVSAIWLLPFYPSPLKDDGYDVSDYTNVHPQYGTIDDFKG
                     FVREAQRRGLKVITELVVNHTSDQHPWFQAARRAPPGSTKRDYYVWSDSDQKYKGTRI
                     IFTDTETSNWAWDPVAKAYYWHRFFSHQPDLNFDNPSVLKAVFRTMRFWLDMGVDGFR
                     LDAIPYLIEREGTSNENLPETHGVLKRIRALIDSSYPNKLLLAEANMWPEDVRPYFGE
                     ADECHMAFHFPLMPRMYMAIAQEDRHPLVEIMQQTPEIPSAAQWAIFLRNHDELTLEM
                     VTSRERDYMYRTYATDLQARINLGIRRRLAPLMENDPERIRLMNSLLLSMPGSPIVYY
                     GDEIGMGDNIYLGDRNGVRTPMQWSPDRNAGFSRADPQRLFLPPIMDPVYGFEAVNVE
                     AQQRDAASQLNWMKRMLATRKASRAFGRGSLEFLRPGNRKILSYLRQYGDEAILCVAN
                     LARTAQPVELDLKRFKGRVPVEMLGRTSFPPIGELPYLLTLPAHGFYWFRLATDAPVP
                     DWHKEMMVSEEAPVLVLFDNWTSFFRDKVVPWRIGMAEKLRTQLENEVLPRFIAAQRW
                     YAAKGEPIQRAVLQDYVIWDVGAISWLLSLTQIKDSLYFLPLALGWEEDEDHVRSLAA
                     VTVARVRQQANVGVLADAIADEGFCRHVVKAIGAGRSVPTANGSLRFTRTRAYAELAG
                     EEVATLQLGPLHTQSTNTSVQLGDRLFLKCYRRLRAGMNPEFEIGRFLTETAHFPHCV
                     PLAGAVEYVSPKESFTVALLQGYVPNQGDGWGYTLGYLERFLESPPQQQPHGGYLTLM
                     QTLATRTGELHRAFAIASGDPAFEPEPLTAQDAAAWKAKVRQEAADTLAFVEKADAER
                     TRALLAQRAELFALIDACAAPKGAGLKTRHHGDYHLGQVLIANHDFVIIDFEGEPSRP
                     LAETRRKHSPLRDVAGMLRSFSYAKWSVRSRQDGGATAKDSALLDAWEADVRRAFLAA
                     YAEATKGSGLYASFEDVAGLLALFELEKVLYELRYEINNRPNWVHVPLSGVLAMLGDE
                     Q"
     gene            18256..18930
                     /locus_tag="VIG70_18385"
     CDS             18256..18930
                     /locus_tag="VIG70_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252566.1"
                     /translation="MNQIDWSLEPLRGFLMRVGDFLPRIALAILIFIAGWIVAKMVRF
                     AIVKGLRAVNFNVLSDRAGIDGFLRDGGIRSDTTEILGLLFYWLVILASLVIGFNLLG
                     LDYITDLLGRVVWFLPKVMVALLILAFGAYFARFVGNAVIAYCRNVHIQDAEILGRIA
                     QYAILAFVVLIALDQVNVGGDIVRQTFLIVLAGIVFALALAFGLGGKEWAAEMLERWW
                     PRRRKP"
     gene            18903..20639
                     /gene="treZ"
                     /locus_tag="VIG70_18390"
     CDS             18903..20639
                     /gene="treZ"
                     /locus_tag="VIG70_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEY8252567.1"
                     /translation="MVAASQEAVNGPNRVARSHSMPFGAQVLAQGGVRFAFWAPGAKK
                     VELEIAGRFMPLHAGRDGWFEHLVSNAGPGTLYRYRIDGEVLVPDPASRFQPRDVDGP
                     SEVIAPRSYPWGDHAWTGRPWNEAVVYEMHVGTFTPEGTYAGAAKKLGYLKELGVTAI
                     ELMPLSDFAGKRNWGYDGVLPYAPDSAYGRPEDLKALIDAAHANGLMVLLDVVYNHFG
                     PKGNYLPRYAPQFFTERHNTPWGAAIDFSNPVVRRYFVHNVLYWLEEYWFDGLRFDAV
                     HAIIDDSKRHILDEIRASVPPGKHLVLENDANQARFIGPGRYSAQWNDDSHHGYHVLA
                     TGESDGYYAAYADAPARHLARCLAEGFAYQGEVSPFSKEPRGEPSAHLPPSCFVDFLQ
                     NHDQIGNRALGERLVALSNEKALKALSAIQLLAPSPPLLFMGEEWGCRQPFLFFCDFE
                     GELGEAVRKGRREEFSRFASFKNSIPDPLAEGTFKQCVLDWAKRDAVWLAHYKKLLQV
                     RARAIAPRTFGPGKYRMLGERAFEVKWDGLTLVANCGDQAIAVKDISKERPLWSNAAA
                     GAPWSVNWWISG"
     gene            20636..>21044
                     /locus_tag="VIG70_18395"
     CDS             20636..>21044
                     /locus_tag="VIG70_18395"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY8252568.1"
                     /translation="MTDEIASSYLDVWGNEKAIEPEVRAALLKAMGPARKARKVEVEV
                     GRCYQPDVLEQRRLWGFMVQLYGLRSERNWGIGDFTDLRKLIEIAAGLGAAVVGVNPL
                     HATQGSPYSPSSRHALNVLYIDVEAVPGYENAGK"
BASE COUNT         3631 a         6978 c         7029 g         3406 t
ORIGIN      
        1 ccgatgagga ccgcggcggc gaacgggccg cgcaggctgc ccaggccgcc gaccgcgacc
       61 acgatcaaaa agtacaccag gtgctcgaac ggataacccc attgcaccgg caggatgtcg
      121 gcgccgagcg cgccgccgag gccggcgagc ccgctgccga gggcgaacgt cgccatgaac
      181 agcagcttgg tgttgatgcc gatcgattgc gccatccccc ggttgtccac cgcggcgcgc
      241 accatcgcgc cccagcgcgt gcgctcgacg ccgaaccaga gcacggcaat gagacatgcg
      301 gagaaagcga tcaggaagac ccggtacgcc ggaaagtcgc ggccgaacac gtgcacctgg
      361 ccgcgcaggt actccggcag gaacaccggc accgtgcggt cgccgtagac gagccgcgcg
      421 atcgccatcg acacgaagat gaggccgatg cagaagagca cctgcgagag ctcgccggcg
      481 gcgtacaggc gcgagtagag ggttctttcg agaacgatgc ttgcgagggc aaccgccgca
      541 aaggcagtga gcagcgcgag cgggaacggc acgcccaggc gtcccatcac cgtggagagg
      601 atgaagcccc ccgccatggc gaacacgccg tgcgcgaggt tgacgaagcc catcaggccc
      661 atcgtcaccg acaggccgac ggaaataacg tacaggatca tgccgtaagc gacgccgtgg
      721 aaggcgacgc ttacgaccga cgaaaagaac tcctgcagag tattacttct tctgcggcgc
      781 ggacttctgc atctctttcc agggatcctt caccgcggtc ccgagcgtct cgatctcgac
      841 gttggcgagc tggccgccga ccttgcgtac ctcgcgcagg tactcgggct ggatgatgtc
      901 gcgcgtctcg gggtcgatcg agatcgggcc gcgcgggctg ttcgggttct tccagccctt
      961 gaagatctcc atcgtccggt ccgggtcgag cttgcccttc tgctgcttga tcgcctcgaa
     1021 aatcagcgcg gtgccgtccc acgagccgac cgagaggaag ttcggcgtct cgccggcgcc
     1081 gaattccttc ttccacgcct cgacgaacgc cttgttcgcc gggcggtcgc ccgcggcgga
     1141 ccagtggaac accgtggtga cgccgagcgc gacgtcgccc atgttgggca gctcttcctc
     1201 ggtggtgatg tcgcccggcc cgatcagctt gatgccggcg ccggcgaggc cgaggtcgga
     1261 gaagttcttc atcactgcgg tcgcggtctt gcccgcgggg atgaacacca tcagcgcgtc
     1321 gggcttcgcg tccttgacgc gctgcatgta ggctgtccag tcggggttct gcagcggcac
     1381 gcgcacgttg gcgacgacgt tgccgccgcc cgccttgaac gcctgcgtga acgcggtctc
     1441 ggcgtcgtgg ccgggaccga agtcgctgac cagcgtgtag gcggtcttgt actttttcgc
     1501 cgcccaggtg ccgagcggga agctcgactg ccacagcgtg aaggaagtgc gcgcgatgta
     1561 cggcgagcgc gtggtgatca ccgcggtgcc ggcgttcatg atgacgaacg gcaccttggc
     1621 ttcggtggcg agcggcgcga tcgcgagtgc gttcggcgtg aagatcacgc cggcgagcag
     1681 gttcaccttg tcgcgcacga tcagctcctg cgcgagctgc ttcgccttgt ccgggttggg
     1741 cccggtgtcg tctcgcgtga tgagctcgat cttcacgccc ggcggcagct ccttctcgtg
     1801 cagcttgatg tacaggcgca ccgcgcgctc catgtacggg ccgaggttgc cgttcaggcc
     1861 ggagtagctg gtgatgaagc cgatcttgac ggtttgcgcc gacgcggaaa acgcgacgcc
     1921 cgaagctgtg gcggcaaacg ctgccgccag cagaaacttc actgatctca ttggtcctcc
     1981 tccttggcgg gggattctag cagccaagtc ccgccgagcg cggccggcag gcgcaccacg
     2041 ttgccgtcga ctttcagtcc cgccttgctg cagcgcgcgg cgaatgcctt cggatcgccg
     2101 caggaaagtg cgtaacccgc gagagccggg gcgggcggcg cctcgccgag aagcttggcg
     2161 agagtctttt gagttccgat cacgacgcgt ccacgcgcag tgtccaggcg gacgaagccg
     2221 tcctccggcc gcggcagcag gccgccgaag cgtccccaac gcgccgcaac ctcctccgga
     2281 tcgtccgcca ctacataaac ggcgcgcagg ccaaacgggt tgacgaaggc ctcgcgccag
     2341 acgtgctcgg gcgtcaggtg ctcgcagtac tggacgcgcc cttcggccat cttgccgggc
     2401 ggcgtgtaga ccaccttgaa gcgcagccgc gcgccggtgt cgatggcccg ctcaaggctc
     2461 accaacggct cgggctcgaa gccgtgtgcg acgagccgag cgtgctcggc ggccgcgtcc
     2521 ggagtgccga agcaggcgag gtgcaccccg tcatagcgct tcatcagcgc gcgcacccgt
     2581 tgtgcgtgag gggtgtccat cgcaggtgcg agcagctcga gatagccttc ttcgagcatc
     2641 acgcatcggt tcgaagttcc cgcgggcttt ccggcaacgg cgtgatgcga aaccggcgtt
     2701 acggtgaacc cgagcttctc ccagaccgcc gccgcggcgc cgagatcggg cacgaagtgc
     2761 gcgagatggt cgaggtagag ctcgcccgca ggcgggcgct ggcggtcggc cgcattcatg
     2821 tcgcgctaat ataaccgcgt gaacaaaccg gtcgagctcg cgacgctgac gggcgccgag
     2881 gcggtcgtcg aaatgctcaa ggcgcacggg gtcgacgtcg tcttcgggct gtgcggcgat
     2941 accagcctgc cgctctacga cgcgctggcg cgcctgggcg ccatcaagca cgtgctgacc
     3001 cgcgacgagc ggcatgccgc ctacatggcc gacggctatg cgcgcctgag cggcaaggtc
     3061 ggggtgtgcg aagggccctc gggcgggggc gcgacctaca tccttcccgg cctggtcgag
     3121 gccaacgagt cctcgattcc ggttctcgcg atcaacaccg acgtgtcggt ttcatcccgc
     3181 gggaagttca cgctgaccga gctggaccag cgcacgctca tgaagccgct gaccaagtgg
     3241 aacgcggtgc tcgaccgctc ggccgacatc ccgaaggttc tccgcaaggc gttcgaatcg
     3301 atgaccacgg gccgcgcggg cgccgcgcat gtcgcgctgc cgttcgacgt gcagaacggg
     3361 ccggtcgagc gcaccgatgt ctgggccgac ccgacgctgg gttcgtttcc ctctcgccgc
     3421 actgcgcccg atcccgccct cgtcgagctg gcagccaagc tcctgcgcaa ggcgaaacaa
     3481 ccgcttttca tctgtggagg cggggtcgtt ctttccggcg cggaaagcga gctactggag
     3541 ctggcggaaa agctctccgc accggtggcc accacgatca gcggcaaggg ctccatcgac
     3601 gagcgctgcg cgcattcggt cggcgtggtc gggtcgaacg gcggcacgcc cgagacgcgc
     3661 gctgtggtcg atgccgccga tttgatcgtg ttcgtcggct gccgcgccgg ctcggtgacg
     3721 accgagcgct ggcgccatcc ggcggcgggc aaggcgcgca tcctccatat cgacgtcgac
     3781 ccggcggtgc cgggcacgaa ctacaaggtc gatgtgccgc tgatcggcga tgcgcggctt
     3841 tgcctctctt ccctcaatca gtccctggag ggataccggc gcgaggccga cctttcctgg
     3901 gtcgatgaaa agaagcgcgc gaagttcgaa aagttcgagg cgctggcgcg ctccgacgac
     3961 acgccgatca agcccgagcg ggtggtgttc gaattgcgga aagccctcga tgcggacgcg
     4021 atcgtggtcg ccgatcccgg aacgccgtgc ccgtacttct ccgcgtacta cgtcgtgaag
     4081 ggctccggcc ggcgctactt ctccaatcgc gcccacggcg cgctcggtta cgcgcttgcg
     4141 gccagcatcg gggcgcatat cgcgaagccg aaggtcaaga cggtcgcggt gatgggcgac
     4201 ggcagcttcg gcatgtgcgt cggcgagctc gagaccgcgg tgcgccttcg cctgccggtc
     4261 accttcgtcg tgatctcgaa cgcggtgtac ggctggatca aggccggcca gaagacgggc
     4321 tacgacgctc gctatttctc ggtggatttc ggcgtcaccg accacgccag ggtggcggag
     4381 gcgttcggcg tgaagagctg gcgcgtattc gacccggcga aactcggcgc cacgctgaaa
     4441 accgccgtag cgcacgacgg tccaacgttg gtggacatcg tctgccagcc gctgcacgaa
     4501 gcaagagcgc cggtctcgga gtgggtggca tgaagagcca tcacgacatg ggcggcgagc
     4561 cgggcgcgaa ggtcgagccg ctcgagcacg actatgcgga ctgggagcgc aggatcgacg
     4621 cgatgggagt gctgctctgg gggatcaagg gcacgaagaa gctcctcacg gtcgacgagc
     4681 atcgcaaggc gatcgagtcg ctggccccag aagcctacga ttcgatggcg tactacgaga
     4741 agtgggtctt cgcgttttcg cagtgcctcg tgcagcgcgg cgtggtcacg agcgcggagc
     4801 tcgcgcgcaa gatgcaggac gtgatgttca tgcgccagca ccacgacctg ggcggccggc
     4861 cggcgggcaa ggtcgagccg accgaacatg actatgcaca gtgggagagg cgcgtggatg
     4921 ccctggcggt cctattgggc agccagaaag tgcttaccgt cgacgagcgc cggcgcgcca
     4981 tcgaatcctt gccgcccgag gcgtacgacg ccatgagcta ctacgaacgc tggaccatgg
     5041 cgctcgcgca gacgctgatc cagcgcggca tcatcacgac cgaagagctc gccaagaaaa
     5101 tgaatgaacg aagctaggtt caaaccgggc gagcgcgtga gggtgatgaa ggccgatccg
     5161 ccgggccaca tccgcacccc ctattacatc cgcggctgtg tgggggaggt cgagcgcatc
     5221 tgcggcgcct ttcccaatcc ggaggagctc gcgcagatgc gaaatggatt gcccgcgcag
     5281 ccgctctata gagtcagatt tttgcaaaag aacatctggc ccgactacgc cggcagcgct
     5341 tcggacgtcc tcgaagtcga gatctatcag cactggctgg aacccgcatg aaagaacaag
     5401 accaggggaa gtaccggccg ctcagctact tccagctcat ggaaatcagc ctgcgcgagc
     5461 tgctggtgga gaaaggcatc ctcaccaatg cccaggtcga cgccgaggtc gagaacatgc
     5521 ggctgcgcac gccagagcgc ggtgcgaggg tcgtggcacg ggcctgggtc gatgcggatt
     5581 tcaagagacg attgctcgag agcggcacga aggcttgcga ggagctcggc ctggacgtcc
     5641 cggcgctcaa gctgctcgcg gtggagaaca cgccgcaagt tcacaacgcg atcgtgtgca
     5701 cgctgtgctc gtgctatccg cgcatgctgc tcggcatccc gcccgactgg tacaagtcgc
     5761 gcaactaccg ctcgcgcatg gtgcgcgagc cgcgggcggt cctcgcggaa tttggcctga
     5821 acctccccga gaaggtgcaa attcgcgtgc acgactcgac cgccgacatg cgctacatcg
     5881 tgctgccgat gcgtcctggc gggaccgagg gctggaacga agagcggctt gcagcgctgg
     5941 tcaaccgcga ctccatgatc ggcgttgcag taccgaaggg ccctcacgat tagcaccgcg
     6001 gtctagaagc ttccatggct caaccccgcc cattgggcgt gatcccgctg ggagactcgg
     6061 cagcgtatgt cgagttctcc gccacgctcg acctcgaggt gaacagcgtc gtccagcgcc
     6121 tcgcgctcac catcctcaag cgcaacgtgc cgtgggtgcg cgacgtggtg ccggcgctcg
     6181 gcggcctcgc gctgcatttc gatccggacc atgcggaatt tccggcaaag ccgctggagg
     6241 aagcgctcgc gctggtgatc gattccctga agcaggccct ggcggccaag gacaatcccg
     6301 gaagcgaagt cgagctgccg gtctgctacg agccggagtt cgccttcgac ctcgccgaag
     6361 tggcgaagaa ggccgacctt tccccggagg aggtggcgaa gcgccacgag gcgggcgagt
     6421 accgcgtgct gatgatggga ttcgctcccg gccacgccta catgggcggg ctcgatgcga
     6481 agctcgccgt gccgcgccgc gcctcgcccc gcgcgatcgt tccggcgggc tcggtggcga
     6541 tcgccaacga gcagacggtg gtctatccct acgcgatctc gggcggctgg aacgtgatcg
     6601 ggcgcacgcc gatggtgctg ttcgacgcga cgcgcacccg cccgagcctg ctgtggccgg
     6661 gcgaccgcgt gcgttttcgc gcgatcagcc gggcagaatt cttgagcctg caaaaaaatt
     6721 catgagcgtg cgcgtgctgc gtcccgggct tctgaccacg gtgcaggacc acggccgcca
     6781 cggcttccag cgcgtcggcc tgtgcccggg gggcgcgatg gacccggttt cgctcgcgct
     6841 cgccaacgcg ctcgtcggca acccgccgga ggaggcggcg ctcgagatca cggtgatcgg
     6901 cccggagctg ctgttcgagc gcgacacgct ggtcgcggtg tgcggggcgg aattccaggg
     6961 cagcttcccg cataaccggc cggtgctcgc gccggccgga acgcgcttca acgtcgggcg
     7021 cgcgacgcgc ggcgcgcgcg cctacatcgc ggtggcgggc gggttcgcgg tcgacccggt
     7081 gctcggcagc cgcagcacct atttgccggg ccacttcggc ggcttcgagg gcagggcgct
     7141 caagcacggg gacgtgctcg cgctgcgcga tggctcggcc gccaagaaat tttccattct
     7201 gaaaaaaacc agggacggca ccgtgaaatg gtcggcgccg ccgctcacgt tgcccgaccg
     7261 cgagccgatc ctggtgcacg tgatcgaggg ccagcacttc gcgagcttcg actcgaactc
     7321 gcagcgcgcc ttcttcgaca ccgtctggcg cgtcgcgccc gactccaacc gcatgggctt
     7381 tcgcctgagc gggccgccgc tcgggcggcc gcaggccgat gagatactat cgggcccgac
     7441 ctgcctcggc tcggtgcagg tgccgccgag cggcgtgccg atcgcgctga tggccgacca
     7501 ccagacgacc ggcggctatc cgcgcattgc cgagatcgcc tccgccgacg tggcgcggct
     7561 cgcgcagctc gcgcccggcg gcaaggtgca cttcgcgaag tgcagcctgg agatcgcagg
     7621 cgagctgcgg cgcgacgcgc gcgcgcgcct cgaatcggcg ctgcgcggca tcgcttggga
     7681 gtacggagca aagaagtgaa gacgatcgac ctgaactgcg acatgggaga gagctacggc
     7741 gcctggaaga tgggcgccga tgccgaagtc atgccgcaca tcagctcggc aaacatcgcc
     7801 tgcggcttcc acggcggcga cccggcaacc attcgcaaga cggtgcgcct tgcgctcgac
     7861 aacggggtgg cgatcggcgc gcacccgtcg ctgcccgaca tccagggctt cggccgccgc
     7921 ccgatgaaga tctcgccgca ggacatgtac gacctcgtcg tgtaccaggc gggcgccgtc
     7981 gaggcgttcg cgcgtgccgc gggcgcgaag ctgcaccaca tcaagtgcca cggggcgctc
     8041 tacaacatgg ccgccggcga cgagggcctg tcggaggcga tggtgcgcgc tgcgaaggac
     8101 cttggcgcga tgctgtacgt cctttcgaag agtcgaaact atgaaatcgc aagaaaggcg
     8161 ggcattccgg tctgcggcga ggtgttcgcc gaccgcggct actccgacga cggctcgctc
     8221 gcgccgcgcg acaagcccgg cggcatgatc gaagacgcag cgaaggcggt gaagcaggcg
     8281 ctgtcgatga tcgaggaggg caccgtcatt agtgtgaatg gcaagcgcgt gccggtcgcg
     8341 gcggacacga tgtgcatcca tggagaccag ccgggcgcgg cgtcgttcgc cagggcgctg
     8401 cggcaggcat tttcggaaag agaaatctct gtcgccgcgc cctaagctct tcacgccgat
     8461 cgagctcggc ggcatcacgc ttcccaatcg catcgtggtc tccccgatgt gccagtactc
     8521 ggccgacgac ggctcgatga ccgactggca cctggtgcac ctcggcacct tcgcctgcgc
     8581 gggggtgggc ctgttcgtgg tcgagaagac gaacgtgacc cgcgatgggc gcatcacgca
     8641 cggctgtacc ggcctctata gcgaccacaa cgaagccgcg atggtgcgtg ccgtgcaggt
     8701 gtatcgcggc attacccgga atccgatcgg catccagatc ggccacgccg ggcgcaaggc
     8761 ctcgtcccac ccgcccttca agggaggcaa gccgctcgga ccgcacgata ccccctggca
     8821 gaccgtgggc ccgtcgccaa ttcctttcgg cgagggctgg catacgccgc atgagctgtc
     8881 ttcttcggaa ttgcgttccc tggttgattc ctatgtcgcg gcgaccgagc gcgccaagcg
     8941 catcggcttc gacgtagtcg agctgcactc tgcccacggc tacctgctgc accagttcct
     9001 cagcccgctc tcgaatcaga gaaaggacga ctacggccgg gaacgaatgc gcttcccgct
     9061 cgaggtggcg catgccgtgc gcgaggtgtg gccgaaggag cgtgcgctcg gcgtgcgcat
     9121 caacgcgacc gactgggtcg acggcggctg gcagccgcag gacgcggtgg cctatgcgcg
     9181 ggagctcaag cgcgccggct tcgatttcgt gtgcgtctcg agcggcgcca cggtgccgta
     9241 cgccaagatt ccggtcgcgc cgggatatca ggtgcctttt tcagagcagg tcagaaggga
     9301 agcaggcgtg gccacgaggg ctgtgggaat gatcgcggat ccggcgcagg ccgaggagat
     9361 cgtcgcttcg gggaaggccg acatggtggc gatggcgcga gcattcctcg acaacccgcg
     9421 ctgggtctgg cacgccgccg agcgcttcgg cgtcgagcta gactatccgc cgcagtacgc
     9481 gcgcagccgc cacgacctgt ggccgggcgc caagcttgcg cgccgttcct gactcgagga
     9541 ggcagccgat ggccgacgag aagagcttcg aagcgagact caggaccgag ggctatcccg
     9601 agatccgcac caaccagctc gagcccaacc gccacaacgc cgagcattcg cacccgttcg
     9661 acgtcctggc gctcgtgctg gaaggcgaga tcaccctgac gaccgaggga cgcggacaga
     9721 cctaccgcgc cggcgacgaa ttttccatgc aagcgggatg caggcacgtc gaagatgcgg
     9781 gtcctcaggg cgtgcgctac ctggtcgggc gccggccgcg atagcaggaa ggagtggagc
     9841 ggggactgag agtcggactc agatctcgat cggggattga ccgcgctctt gccattgagc
     9901 tacccccgct tcaggacgaa gtttgaagcg tcagccgcgc gtcagcaata gtcgaaccgg
     9961 cgcatctttc acgagccttt gacgactttg atctggttgt cgacggacga caccccgtcg
    10021 accttccccg cgatctgtcc ggcgcggttg cgctctcccg ccgtcgcggc cgtgccccac
    10081 agactgacgc ggccttcttt cgcggtcacg tcgatccccg cagcctggac cttgccgtcg
    10141 ccttcgagcg cctgcttcac ccgctgcgcg agctccttgt tcgggtccgg tttcggcgcc
    10201 tcgggtgctt tggtttcggg agcctttggt gccggcgcgg cggcttgcgg tgccggcgcg
    10261 ggtgcggctg gctgggcggc cggcttgggt gggggcgcgg aatcgccgca ggcggcgagc
    10321 agcaagatca aagcagcggc atgaatacgt gtcatgtatg gacctcctga cccgaaagac
    10381 tacccccgga tcgtggcgta gtcatgcgcg tttgtagctt ttcaatgaca agcgaacaga
    10441 tcggcgatcg cgcgagcgtc gaagcggtac tcgtggttgc acatctcgtt gcggatcacc
    10501 acctcgccct gctcggcgag gatcgactcg acctcatcgc ggccgaggcc gagcagcatg
    10561 tccttgacct tggtctcgtc gtaggggcag tggtactcga ccgcgtagag ccggaacacg
    10621 cgcatcagct cctccggaaa cacgcgcgtc agcaggtcgt agggctgcgc gtcgcgcgtc
    10681 tcgtccaggg tcagggtgtt ggcgaggtgc atgacccgat tccagccgtc gggatcgcac
    10741 aggtcggcgc gcggcagctt ctccagaagc agcccgcaaa gcgcgccgcc gtcggcccgc
    10801 agccgcagga acgcggtcgt ctgctcggac tgcgacaggt aatgctcgaa gatctgctcg
    10861 agggtctcgc cctccagcgg caccaggctc tggtagaact ggccgctgtg gacgtcttcg
    10921 agggtcacgg acaggcggcc gttcccgagg agggctcttt cgttgaagga gctcgccggc
    10981 ttctcgtagc tggccatccc ccggatccgc agctcggcgg tgcagtcgga gaccagcagc
    11041 ttgaccgggc ccgagccttg cacctggagg gtgacccgac cggagccctt ctgatttgcc
    11101 cccaaaagga cgttcagggc ggtggtatga cccagcagtg ccgcgatatc ctcggggtag
    11161 tcccggccat ccagcatgcg ctgccaggcc ccggtgaggc agaccaagcg tccccggatg
    11221 tcgagatcct cgaaaaggaa ccgctgaacg tagtccgtct gcatggtcaa aagtatagat
    11281 gcctaccgtc gctgaccgcc ccataagtct gaccggacac gacctgtacc tgttccggga
    11341 gggcacgcac agccggctct acgagaagct gggtgcccat gtgctcgggg atggaacgca
    11401 gttcgcagtg tgggcgccga acgccgctca ggtgtctgtg atcggcgact tcaacgactg
    11461 ggacccgcgc gcacacccca tgggttcgag cgaggccggc gtctggtcgg cgaaggtgcc
    11521 cgaggcaaag cagggcagcg tctacaagtt ccacgtcgtc tctcggcacg gcaaccaccg
    11581 cgccgacaag gccgacccgt atgcgttccg ctgcgagacc ccgccgcgca ccggctccgt
    11641 ggtgtggaag ctcgactacg agtggaacga cgccgagtgg atgaagaccc gctcgcgcgc
    11701 caacgcgctc gacgcgccgt ggtcggtcta cgagctgcac ctcggctcgt ggcgccgcga
    11761 tccgtcggat ccgcgcaggc tcctgagctg cagggagctc gctccgatgc tggccgagta
    11821 ctgcaagcgc atgggcttca cccacgtcga gctgatgccg gtgatggagc acccgttcta
    11881 cggctcgtgg ggctaccagt gcaccggcta cttcgccccg agctcgcgct acggcacgcc
    11941 gcaggacttc atgcacctcg tcgacgtgct gcaccagaac ggcatcggcg tgatcctcga
    12001 ctgggtgccg tcgcacttcc cctgggacca gcacggcctg ggcttcttcg acggcacgca
    12061 tctctacgag cattccgacc cgcgccaggg gcatcacccg gactgggcga gcgcgatctt
    12121 caactacggc cgcaacgaag tgcgcgcctt ccttccctcg agcgcgcact tctggctcga
    12181 gcgcttccac gccgacgggc tgcgcgtcga cgcggtcgcc tcgatgctct acctcgatta
    12241 cgggaggcaa gcgggcgagt ggatccccaa ccgccacggc gggcgcgaga acctcgacgc
    12301 gatcgacttc ctgcgcttcc tgaacgagac gctgtatcgc gactttcccg acattcagac
    12361 gatcgcggag gagtcgaccg cgtggccgca ggtgtcgcgc ccgaactatg tcgggggcct
    12421 cggcttcggc atgaagtgga acatgggctg gatgcacgac acgctcggct acttcaagcg
    12481 cgacccggtg taccgcaagc accaccacga cgagctcacc ttcagcctgt ggtacgcgtt
    12541 ccacgagaac ttcgtgctgc cgctttccca cgatgaagtc gtgcacggca agagctcgct
    12601 catcgggcgc atgcccggcg acgcctggca gcagttcgcc aacctgcgcc tgctcttcgg
    12661 caccatgtgg gcgcacccgg ggaagaagct gctcttcatg ggcggcgagt tcggccagcg
    12721 gcgcgaatgg acgcacgagg acagcctcga gtggcacgtg atcagccagg acacgcgcca
    12781 cgagggcgtg caacgctggg tcgccgacct gaatcgcgtc tacagaaatg agaagtccct
    12841 gcatgaaaag gacttctcgg gcgaaggctt ccgctggatc aaccgcggcg actgggaggc
    12901 gagcgtgctt tccttcctga ggcaaagccc gggcagcgac ccggtgctcg tggtctgcaa
    12961 cttcaccccc ctgccgcgcg tcaactatcg cgtcggcgtg cctcgcggcg ggcgctggcg
    13021 cgagctcctc aacagcgacg cccagtacta tggcggcagc ggcatgggca accagggcgc
    13081 cgtggaggcg caacccatgc cgtacgagga actgagccac tccctgaccc tcaccctgcc
    13141 gccgctcgcg gtgctcttct tcaagcctga ataagtcgcg cgacaaaatc cggacggcgc
    13201 cggcgcgcgt cgtcatcgag tcggtccggc cgcaggtcga tggcgggcgc tacccggtca
    13261 agcgcgccgt cggcgacgag gtgctggtcg aggcggatgt cttcaccgac ggccatgatg
    13321 tcgtcgtggc gcagcttctg ttcaagttcg accaggacaa ggaatggaaa accctgccga
    13381 tggagttccg ccacaacgat cactgggcgg cgtcgttccg cgtcgagaag ctgggccgct
    13441 accagtacac ggtgcgcggc tggaccgatc cgttcctcac ctggcgtcgc gacctcgtca
    13501 agcggcgcga cgcgggccag gacctgacga tcgatctgca gatcggcgcc gggatcctcg
    13561 gcgcggaaat cccgagctac gaagccgcat tggacgtcga cgcacctcga ccccgcgaag
    13621 agtggatcgt gacccatgag aaagtcctcg aagtcgtcgt cgacccggtc cgcgcgcgct
    13681 tcagctcgtg gtacgaaatg ttcccgcgct cgttcggcgg tttccggggg atgatcgaag
    13741 tcctgccgca cgtcgagcgc ctcggcttcg atgtcctcta tctgccgccg gtgcacccca
    13801 tcggcaccac ggcgcgcaag gggaagaaca acgcggcgaa agcgctgccg ggcgacaccg
    13861 gcagcccatg ggcgatcggc tcgaaggagg gcggccacaa ggcgctgcat ccggagctgg
    13921 ggaccttcga agactttcac gccctggtcg gggccgccga aaaacgcggc atccagatcg
    13981 cgctggacat cgccttccag tgctcgcccg accatccgta cgtgaaggat cacccggagt
    14041 ggttccgggc ccgccccgac ggcaccatcc agtacgcgga gaacccgccg aaaaagtatc
    14101 aggacatcta cccgttcgat ttcgaaagcg agaactggac cgagctctgg aacgagctga
    14161 aaagcatctt cgagttctgg gtcggccacg gcgtgaagat cttccgggtc gacaacccgc
    14221 acaccaaggc gttcgagttc tgggagtggg cgatctcgga tctgaagcgc aagcatcccg
    14281 agctcatctt tctctccgag gcgttcaccc gcccgcgcgt gatgcacaag ctggcaaagc
    14341 tgggcttcac gcagtcgtac acctacttca cctggagaaa caccaagcag gagctggcgg
    14401 agtacttcac ggaagtgacc caggattcct cgcgcgagta cttccggccc aacttctggc
    14461 cgaatacgcc cgacatcctc cacgagacgc tgcagcacgg tggccgcccg gcgttcatcg
    14521 tgcgcacggt gctcgcggcc atggcggcgg cgaactacgg catgtacggc ccggcgtacg
    14581 agctgcagga gcgcacgccg cgcgagccgg gcagcgagga gtacctgaac tcggagaaat
    14641 acgagatcaa ggcctgggac ctgaatcgct ccgacagcct ggcgccgctg atctcgcgcc
    14701 tcaatgccat tagaaagcag aatccggcgc tgcagtcgga ctggagcctc aagctccatc
    14761 cgatcgacaa cgaccatctg ctggctttca gcaagaagga aggggacaac gagatcctgg
    14821 tcgtcgtgaa cctcgacgtg caccatccgc agtcgggctt tgtcgatttt ccgccgtgcg
    14881 aagtgcacga tctcctctcc ggcgggcatt acgcgtggag ctccggcaga aactacgtcg
    14941 agctcagccc gcacaagctg ccggcgcacg tgttccgcgt gacgaaaaaa tgaacgagcg
    15001 ggccacgcct tcgctcgtca cgccgccgct cgaggccgcg gtggaaaccg acgccctctg
    15061 gtacaaggac gcggtcatct accagcttca cgtcaaggcg ttcttcgact cgaacaacga
    15121 cggcatgggc gacttcggcg ggctgatgca gaagctcgac tacatccagg agctcggcgt
    15181 ctcggcgatc tggctgctgc cgttctaccc ctcgccgctc aaggacgacg gctacgacgt
    15241 ctcggactac accaacgtgc acccgcagta cggcacgatc gacgacttca aggggttcgt
    15301 gcgcgaagcg cagcgccgcg gcctgaaggt gattacggaa ctggtcgtga accatacctc
    15361 cgaccagcat ccgtggttcc aggcggcacg ccgcgcgccg ccgggctcga ccaagcgcga
    15421 ctactacgtg tggagcgact cggaccagaa gtacaagggc acgcgcatca tcttcaccga
    15481 caccgagacc tcgaactggg cctgggaccc ggtggcgaag gcgtactact ggcaccgctt
    15541 cttcagccac cagcccgacc tcaacttcga caacccgagc gtgctgaagg cggtgttccg
    15601 caccatgcgc ttctggctcg acatgggcgt cgacggcttc aggctggatg cgattcccta
    15661 cctgatcgag cgcgaaggca cttcgaacga gaaccttccc gagacccatg gggtgctcaa
    15721 acgaattcgc gcgctcatcg acagcagcta cccgaacaag ctgttgctgg cggaagccaa
    15781 catgtggccc gaggacgtgc gcccgtactt cggcgaggcc gacgaatgcc acatggcgtt
    15841 ccacttcccg ctgatgccgc gcatgtacat ggcgatcgcg caggaggacc gccacccgct
    15901 ggtcgagatc atgcagcaga cgcccgagat ccccagcgcc gcgcagtggg cgatcttcct
    15961 gcgcaaccac gacgagctga cgctcgagat ggtgacgagc cgcgagcgcg actacatgta
    16021 ccgcacctac gcgaccgacc tgcaggcgcg catcaacctc gggatccggc ggcggctcgc
    16081 gccgctcatg gagaacgacc ccgagcggat ccggttgatg aacagcctgc tcttgtcgat
    16141 gccggggtcg ccgatcgtgt actacggcga cgagatcggc atgggcgaca acatctacct
    16201 cggcgaccgc aacggcgtgc gcacgcccat gcagtggagc ccggaccgca acgcgggctt
    16261 ctcgcgcgcc gatccgcagc gcctcttcct gccgccgatc atggacccgg tctacggctt
    16321 cgaagcggtc aacgtcgaag cgcagcagcg cgacgcagcc tcgcagctca actggatgaa
    16381 gcgcatgctc gcgacgcgca aggcgagccg ggccttcggc cgcggaagcc ttgaattcct
    16441 gcggcccggc aacagaaaaa tattgagcta cttgagacag tacggcgacg aggcgatctt
    16501 gtgcgtggcg aacctcgcgc gcaccgcgca gccggtcgag ctggatctca agcgcttcaa
    16561 gggacgcgtg ccggtcgaga tgctggggcg cacctcgttc ccgccgatcg gcgagctgcc
    16621 gtacctcctg acgctgccgg cgcacggctt ctattggttc cgcctcgcca ccgacgcgcc
    16681 ggtgcccgac tggcacaagg aaatgatggt ttccgaagag gcgccggtgc tggtgctgtt
    16741 cgacaactgg accagcttct tccgcgacaa ggtggtccct tggcgcatcg gaatggcgga
    16801 aaagctgcgc acgcagctcg agaacgaggt cctgccgcgc ttcattgccg cgcagcgctg
    16861 gtatgcggcc aaaggcgagc cgatccagcg cgcggtcctg caggactacg tcatctggga
    16921 cgtgggcgca atcagctggc ttttgtcttt gactcagata aaagattccc tgtacttcct
    16981 gccgctggcc ctgggctggg aggaggacga ggaccacgtg cgctcgctcg ccgcggtcac
    17041 ggtggcgcgc gtgcgccagc aggcgaacgt cggcgtgctc gccgacgcga tcgccgacga
    17101 gggattctgc cgccacgtgg tgaaagcgat cggcgccggg cgcagcgttc ccaccgcgaa
    17161 tggctcgctg cgcttcactc gcacgcgggc gtacgcggag ctcgcgggcg aggaggtcgc
    17221 cacgctgcag ctcgggccgc tgcacacgca aagcaccaat acctcggtgc agctcggcga
    17281 tcgcctcttc ctcaagtgct accggcggct gcgcgccggc atgaatcccg agttcgagat
    17341 cgggcgcttc ctcaccgaga cggcgcattt cccgcattgc gtgccgcttg ccggagcagt
    17401 ggaatacgtc tccccgaagg aatcgttcac ggttgcgctg ctgcagggct acgtgccgaa
    17461 ccagggcgac gggtggggct acaccctcgg ctacctggag cgattcctcg agtcgccgcc
    17521 ccagcagcag ccccacggcg gctacctcac gctgatgcag acgctcgcca cgcgcaccgg
    17581 cgagctgcac cgcgcgttcg ccattgcgtc gggcgacccg gcgttcgagc ccgagccgct
    17641 gaccgcgcag gacgcggccg cgtggaaggc gaaggtgcgg caggaggccg cggacacgct
    17701 ggcgttcgtg gaaaaggcgg acgcggaaag aaccagggcg ttgctcgcgc agcgcgccga
    17761 gctctttgcg ctaatcgacg cgtgcgcggc gccaaagggc gccggtctca agacccgcca
    17821 ccacggcgac taccacctcg ggcaggtgct gatcgcgaac cacgacttcg tgatcatcga
    17881 tttcgagggc gagccgtcgc ggccgctcgc cgagacgcgg cgcaagcatt cgccgctgcg
    17941 cgacgtcgcc ggcatgctgc gctcgttcag ctacgcgaag tggagcgtac gaagccggca
    18001 ggacggcggc gccacggcga aggattcggc gctgctcgat gcctgggagg cggacgtgcg
    18061 ccgcgcgttc ctcgcggcct acgcggaagc gacgaaaggc agcgggctct acgcgtcgtt
    18121 cgaggacgtc gcgggcctgc tcgcgctgtt cgagctcgag aaggtgctct acgagctgcg
    18181 ctacgaaatc aacaaccgtc ccaactgggt ccacgtgccc ctgtccgggg tgctcgccat
    18241 gctaggagac gaacaatgaa ccagatcgac tggtcgctgg agccgctgcg cggcttcctg
    18301 atgcgcgtcg gagacttcct gccgcgcatc gcgctcgcca tcctcatttt catcgcaggc
    18361 tggatcgtcg ccaagatggt gcgcttcgcc atcgtgaagg gcctgcgcgc ggtgaacttc
    18421 aacgtgctct ccgatcgcgc cggcatagac ggcttcctgc gcgacggcgg catccgctcc
    18481 gataccaccg agatcctggg gctgctgttc tactggctgg tgatcctggc gtcgctggtg
    18541 atcggcttca acctgctcgg cctcgattac atcaccgacc tcctcggccg cgtggtgtgg
    18601 ttcctgccga aggtgatggt ggcgctcctg atcctcgcct tcggcgccta tttcgcccgc
    18661 ttcgtcggca acgcggtgat cgcctattgc cgcaacgtgc acatccagga cgcggagatt
    18721 ctcgggcgca tcgcccagta cgccatcctc gctttcgtcg tcctcatcgc gctcgaccag
    18781 gtgaacgtcg gcggcgacat cgtgcgccag accttcctca tcgtgctcgc ggggatcgtg
    18841 ttcgcgctcg cgctcgcctt cggcctcggc ggcaaggaat gggccgccga aatgctcgaa
    18901 cgatggtggc cgcgtcgcag gaagccgtga acgggccgaa tcgcgttgcg cgaagccatt
    18961 ccatgccctt cggcgcgcag gtcctggcgc aagggggcgt gcgcttcgcc ttctgggcgc
    19021 cgggcgcgaa aaaggtcgag ctcgagatcg cgggccgctt catgccgctg catgccggcc
    19081 gcgacggctg gttcgagcac ctcgtgtcca acgccgggcc cggcacgctc taccgctacc
    19141 gcatcgacgg cgaagtgctg gttcccgatc ctgcttcgcg cttccagccg cgcgacgtcg
    19201 acgggccgag cgaggtcatc gcgcctcggt cgtacccctg gggcgaccac gcgtggaccg
    19261 gccgcccgtg gaacgaggcc gtggtctacg agatgcacgt ggggaccttc actcccgaag
    19321 gcacttacgc cggcgcggca aagaagctcg gctatctaaa ggagctcggc gtcaccgcga
    19381 tcgagttgat gccgctgtcc gacttcgcgg gcaagcgcaa ttggggctac gacggcgtcc
    19441 tgccctacgc gccggactcg gcctacggcc gcccggagga tctcaaggcg ctgatcgacg
    19501 cggcgcacgc caacggcctg atggtgctcc tcgacgtcgt ctacaaccac ttcggcccca
    19561 aggggaacta cctgccgcgc tacgcgccgc agttcttcac cgagcggcac aacaccccat
    19621 ggggcgcggc gatcgatttc tcgaacccgg tcgtgcgccg ctacttcgtg cacaacgtcc
    19681 tctactggct ggaggagtac tggttcgacg gtctgcgctt cgatgcggtc cacgccatca
    19741 tcgacgactc gaagcgccac atcctcgacg agatccgcgc ctcggtgccg cccggcaagc
    19801 acctggtgct cgagaacgac gccaaccagg cgcgcttcat cgggcccggg cgctacagcg
    19861 cgcagtggaa cgacgattcc catcacggct accacgtgct cgccacgggc gagtctgacg
    19921 gctactacgc cgcctacgcc gatgcgccgg cccggcatct cgcgcgctgc ctcgccgaag
    19981 ggttcgcata ccagggcgag gtgtccccgt tcagcaagga gccgcgcggc gagccgagcg
    20041 cgcacctgcc gccgtcctgc ttcgtcgact tcctgcagaa ccacgaccag atcggcaacc
    20101 gggccctcgg ggagagattg gtcgcccttt cgaatgagaa agccttgaag gccctgagcg
    20161 cgatccagct cctcgcgccc tcgccgccgc tgctcttcat gggcgaagag tggggctgcc
    20221 gccagccgtt tctcttcttc tgcgacttcg agggcgagct cggcgaggcg gtgcgcaaag
    20281 gccggcgcga ggagttcagc cgctttgcct ccttcaagaa ttccatcccg gatccgctgg
    20341 ccgagggcac gttcaagcaa tgcgtcctcg attgggccaa gcgcgacgcg gtctggctcg
    20401 cgcattacaa gaagctcctg caggtccgcg cgcgcgccat cgcgccgcgc acgttcggcc
    20461 cggggaagta ccgcatgctc ggcgagcgag cgttcgaggt gaagtgggac gggctgacgc
    20521 tggtggccaa ctgcggcgat caggccatcg cagtaaagga catttcgaaa gagaggccgc
    20581 tgtggtccaa cgcggctgcg ggcgcgccct ggtcggtgaa ttggtggatc agcgggtgac
    20641 agacgaaatc gcgtccagct atctggacgt gtgggggaac gagaaggcaa tcgagcccga
    20701 agttcgtgcg gcgctgctga aggcgatggg gccggcgcgc aaggcgcgca aggtcgaggt
    20761 cgaggtgggg cgttgctacc agccggacgt gctcgagcag cgccggctgt ggggattcat
    20821 ggtgcagctc tacggcctgc gctcggagcg caactggggc atcggcgact tcacggacct
    20881 gcgcaagctg atcgagatcg ccgcaggtct cggagcggcg gtcgtgggcg tgaacccgct
    20941 gcatgcgacg caaggcagcc cgtacagccc gtcgagccgc catgcgctca acgtgctcta
    21001 catcgacgtc gaagcggtgc cggggtacga gaacgccgga aagc
//
LOCUS       DATBSZ010000284        33228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1218872, whole genome shotgun sequence.
ACCESSION   DATBSZ010000284 DATBSZ010000000
VERSION     DATBSZ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 33228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33228
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218872"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VIG70_18400"
     CDS             <1..445
                     /locus_tag="VIG70_18400"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY8252569.1"
                     /translation="GRLCDLAARYRLAANVEPMPWVEVSSVSKTKRVLDQAARKNSAL
                     LVDAIHFFREENKLEQLQEIRLNYMQLCDARPERPADMQEIIRQARADRLFPGEGGLD
                     LQGLMRALPRELPISVEIPYAKPMAPLERAQRALAATRLLLSRVA"
     gene            complement(422..1495)
                     /locus_tag="VIG70_18405"
     CDS             complement(422..1495)
                     /locus_tag="VIG70_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEY8252570.1"
                     /translation="MTTVRKMTQYVPLTREQFRSRFFERFYDPAFDAVKAELEKVCEV
                     AWDGYIKYRKSPRSQPAGSEFSDPGFKLPVEWLQARAAILQASKRQTDPASASRILVV
                     SGATRSEHTCPGEVSKTRRLADRARRTIEAQAGFEVDFLELSQLADEPWKVIHPCKAC
                     VSTSMALCHWPCSCYPNHAMGQTNDWMAELYPRWVAAHGVFVLCPVHWYQAPASLKLM
                     MDRLVCADGGNPDPTTTRGKDPAAAKALELAGWPYPRHLAGRAFAVVAHGDSQGHEGL
                     REALVEWLASIGMIQAGVSAAVDTLIGYWEPYATSHDKLDSQSETFVQVENAAKSLVN
                     MVRAIRSGTYRAPDEGLRNPRQK"
     gene            complement(1492..2220)
                     /locus_tag="VIG70_18410"
     CDS             complement(1492..2220)
                     /locus_tag="VIG70_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEY8252571.1"
                     /translation="MGWLTNWRRRRVLDRHRIDEALWNEATTGLPFIPKSEKLRELAL
                     LFLAEKQFTGAHGLEVTDAMRISIAAQACLPILELGLDWYAGFTGIVVYPGDFRVRRE
                     ETDEHGVVHEWDDELAGEAMPGGPVVLSWDATANDPEINVVIHEFAHKLDMLNGAADG
                     LPPLHAGMDRAAWQAAFAQAYEGFCDAVERDKETWLDPYAAEHPSEFFAVISEAFFEQ
                     PAETKRRYPDVYDQLRLFYRQDPS"
     gene            2238..4361
                     /locus_tag="VIG70_18415"
     CDS             2238..4361
                     /locus_tag="VIG70_18415"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY8252572.1"
                     /translation="MVSVVQLHPQSGAAALGPLADGLAAPERALIGRALEFAEPLYAG
                     QVLSTGEPCWSHALGLAASLAAIGLDAPGRAAGVLFAAPKYLDGTEKLGEAFGAEIAA
                     LAAGVEKLYQLRVATRGTPSEQNEVLRKMVLGMVEDVRVVLVRLASRTQTLRFFAKNP
                     SPERASYARETLDIYAPLANRLGVWQLKWELEDLSFRYLEPELYKRIAQMLDEKRLER
                     EQYIANALALLRTELKAIGLDSNVSGRPKHIYSIWNKMRSKELDFSEVYDVRALRVIV
                     PEIKDCYAALGVVHNLWQPIPKEFDDYISRPKGNLYQSLHTAVLGPGGKTLEVQIRTE
                     EMHKQAEYGVAAHWQYKEKAKASKQFEQKVAWLRQLLAWRDEVPIKRPELEDTIYVLT
                     PQGKVIDLPAGATPIDFAYALHSDLGHRCRGARVDGHIVKLDTPLASGQRVEIVAAKS
                     GGPSRDWLNPERGFVKSVRSRHKIRQWFNAKALAETVAAGRAVVEKELRREGATQANL
                     EGLAAKLGFAKPDEMFAAVARDEVNLRQLQVALKGAAPPVSAPELKRAKKPPSAKASM
                     LVVGMGGLATQLARCCKPVPPDPIRGFVTRGKGVSIHRESCDGLKRLAERHPERVMET
                     DWGKSDAAYAVDMTVTATDRRGLLRDIGDALAREKINLTAVRTQTRDDLAFMRFTFDV
                     ADSGQLKRALVLVRGLKGVIRVARG"
     gene            4456..4842
                     /locus_tag="VIG70_18420"
     CDS             4456..4842
                     /locus_tag="VIG70_18420"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY8252573.1"
                     /translation="MLIGRILLAAVFLVAGARKLMGVAASAGYLAKLGFPAPEIMVWV
                     AIVIEIGGAVLLITGWHARRAAWLLILFVAIATAMAHRFWEFDAAQYNNQLNHFLKNA
                     AIIGGLLYVAMLGAGALSLGARSRPS"
     gene            complement(4846..5238)
                     /locus_tag="VIG70_18425"
     CDS             complement(4846..5238)
                     /locus_tag="VIG70_18425"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEY8252574.1"
                     /translation="MHARDSHVEESPHKGKTGLRRVLNATRYSFAGLAAAARYESAFR
                     ELAALCLVAVPAAFWLGDTGAERALMIGSVILAMIVELVNAAVEATVDRISLENHRLA
                     KRAKDIGSAAVMLSLVNAGVVWLLIILS"
     gene            complement(5214..7304)
                     /gene="ppk1"
                     /locus_tag="VIG70_18430"
     CDS             complement(5214..7304)
                     /gene="ppk1"
                     /locus_tag="VIG70_18430"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY8252575.1"
                     /translation="MNRRVPVPAQFLNRELGILAFNRRVLAQAADDTVPLLERLRFIT
                     IVSSNLDEFFEIRVAGLKEQIKLGLPEPGPDGGAPAEVFESVSREAHALVAEQYQLLN
                     QTVLPALAAQRIVFPKREDWNAAQRQWIRDYFFRELLPVLTPIGLDPAHPFPRVFNKS
                     LNFALELEGRDAFGRSSGAAIVQAPRALPRLIRLPQAIAGDAEYAFVFLSSILHAHMD
                     ELFSGMNVQGCYQFRVTRNSDLFVDEEEIKNLHTALQGELFHRHFGDAVRLEVADNCS
                     QRMVEFLLQQFGLREADLYRVEGPVNLYRLREVPDQVARPDLKYPLFQPGLPPALDTG
                     DIFEVLKKQDVLLHHPFQSFLPVIDFIRTTANDPQVIAIKQTVYRTGTDSELMEILVE
                     AARKGKEVTVVVELMARFDEEANLNWAARLEEVGAHVVYGVVGHKTHAKMALVVRRED
                     GGDGGARVLRRYAHLATGNYHPRTARLYTDFGLLTANEEMCADVAEVFKQLTGLGRAG
                     RMKHLWQSPFTLHKRIVAAIRNEAAQAAEGKPARIIAKMNALLEPQIIEELYAASAAG
                     VKIDLIVRGVCALKPGIQGLSENIRVRSVVGRFLEHTRIFFFHDGGAENVYLSSADWM
                     DRNFFRRIELCFPVLDPALKRRVIREGLQPYLDDSSQVWEMSAEGAYERVKPRRGRRR
                     SAQEELLFTLGIPT"
     gene            7557..8261
                     /gene="phoU"
                     /locus_tag="VIG70_18435"
     CDS             7557..8261
                     /gene="phoU"
                     /locus_tag="VIG70_18435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY8252576.1"
                     /translation="MTTDHTSKDFDHALDRARELVSLMAVRVERQLADAIECLQTGSP
                     ALIAQILRHEAEVNGLERAVDEVGGQIIARRHPAARDLRLVMSLLGATTDLERVGDEA
                     KKISLYARSIFGAGRQVLPRVLELRPMGELVHDMLRQATRAMEALDPNAAAALVRRDL
                     DVNERFRAVLARLAGFMVEDPRSISACLDILFVAKSFERIGDHAKNLGEHVVYATIGK
                     DVRHATAQEIEQVVRS"
     gene            8281..9801
                     /gene="ppx"
                     /locus_tag="VIG70_18440"
     CDS             8281..9801
                     /gene="ppx"
                     /locus_tag="VIG70_18440"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEY8252577.1"
                     /translation="MARHDTLAAVDLGSNSFHLEIGRVVDGQIYPLDAVREVVRLGGG
                     LTAEKRIDRATQATALEAIAKFGERLRGFPRPAVRAVGTNTLRVAKNAPQFLREARAA
                     LGFPIEVISGREEARLIYLGVAHSLPASAQKRLVVDVGGGSTEFIIGTGYDALLTESL
                     YMGCVSYSLKYFPEGKVEKSGMKAAELAARQELAALVKEYRDTGWEEAVASSGTARSI
                     ENILRENELAAEGLTREGLDRLRSLLIKAEKADPDRIRGLRPNRAPVLPGGLAIMIAV
                     FDALGLESMKVSEGALRHGVLYDLLGRVQHRDMRAATVAQFMRRYHIDVPQADRVRAL
                     ALTIYDALTPGGDREDDPDRVMLEWAARLAEVGLSIAHAAYHKHSAYVLSNADMPGFS
                     RMEQARLARLALAHRGKLGKVQDAGFESADWKLVFGLRLASLILRSRSDAKLPFLRVA
                     ADAGGFAIDLPQSWLEENPLSADALDAEARHWKAVGMKLEVNGLSDRKVSVLKQVR"
     gene            complement(9784..10233)
                     /locus_tag="VIG70_18445"
     CDS             complement(9784..10233)
                     /locus_tag="VIG70_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8252578.1"
                     /translation="MDLIIWRHAEAEDGVPDLERKLTAKGRKQAERVAQWLLQRLPAR
                     FAVVASPAARAQQTAEALGVPVKTDRTLAPGASPSTIIQACGWPDYKGATVIVGHQPD
                     LGRVLAELLGARGSFSIKKGGLWWISNRVRDEKDQVVVRATVSPDLL"
     gene            10265..10341
                     /locus_tag="VIG70_18450"
     tRNA            10265..10341
                     /locus_tag="VIG70_18450"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10299..10301,aa:Val,seq:gac)
     gene            10358..11317
                     /locus_tag="VIG70_18455"
     CDS             10358..11317
                     /locus_tag="VIG70_18455"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8252579.1"
                     /translation="MKLRAFAFRFVFLCAFSSAALAQFPARAVTIVVPIPPGGAPDIA
                     ARVIGQKLSENIGQPVVVENKVGANGNIANELVARAQPDGYTLGLLADSQIAINPHLY
                     KMPIDTLRDLTPVTPVAVNQFVLTVNPSLPVRTFPEFIEYAKKANPPLAYASGGNGSQ
                     HHLTMEMLKQRAGINLLHVPYKGGAPAATATVAGDTQAMWSGSSNAPQIKAGRLRPLA
                     VSGAHRSAQYPELPTIGEFYPGLENSIWLGLFGPAGIPEATLGRLRAELKRVLESPDV
                     KEKLNAAGGLDPYIASPEEFAALIKRDYAKYAKVVKDIGIKLD"
     gene            complement(11342..12277)
                     /locus_tag="VIG70_18460"
     CDS             complement(11342..12277)
                     /locus_tag="VIG70_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-dihydroxyphthalate decarboxylase"
                     /protein_id="HEY8252580.1"
                     /translation="MKLPLSLAISPYDHARDLRPQGIDLTVLDLSVEEIFYRFTRFRE
                     WDASEMSFGKVVSILSLPSPDLIALPVFPSRVFRHSAIYTKLKTPKELEGKRVGIPEW
                     AQTAGIYVRGLLQHEYGVDLRRIEWIQAGVHQPGREEKVELKLPQGVRITPQPGKTLA
                     GMLAGGELDAVISARDPGGPRMFSDYAGLELEYFRKTRIFPIMHVVVLRREAYERDRW
                     IAMNLFKAFEEAKRVSQARLTEIGISHIPMPWLAEHARRWREAAGEDSWPYGIEPNRP
                     TLEAFVQYAFEQGVATRPLKIEELFAPETLERAKI"
     gene            12306..14222
                     /gene="thrS"
                     /locus_tag="VIG70_18465"
     CDS             12306..14222
                     /gene="thrS"
                     /locus_tag="VIG70_18465"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY8252581.1"
                     /translation="MPNIRLPDGSSKSFPGPVTVAEIASSIGAGLARAALAAKVDGKL
                     VDTSYRVERDATISIVTERDPEGLEIIRHSTAHLLAQAVKELFPEAQVTIGPVIDNGF
                     YYDFSYKRPFTPEDLAAIEKRMAEISKQDLPVHRREMARDEAVAYFKSLGENYKAEII
                     ASIPGKETISLYGQGNWIDLCRGPHVPSTGKLKTFKLMRVAGAYWRGDSKNEMLQRIY
                     GTAWAKKEDQDAYLKMLEEAEKRDHRRLGTQLDLYHMQEEAPGLVFWHPKGWTLWQAV
                     EQYMRRVYRDNGYHEVRGPQILDVELWKRTGHWDNFRENMFFTASENRDYAVKPMNCP
                     GHILIYNKGVRSYRELPLRYGEFGSCHRNEPSGALHGLMRVRGFVQDDGHIFCTEEQI
                     LGECVAYTALLQKVYRDFGFTDIIYKVATRPAKRIGSDESWDRAEHALKTSLEKSGVQ
                     FSVSPGEGAFYGPKIEYSLKDGIGRVWQCGTMQVDFAMPARLGAEYVAEDNTRRTPVM
                     LHRAIVGSFERFIGILIEHYAGAFPLWLAPHQLVVMNITERQAEYALSVKNKLVNQGF
                     RAILDLRNEKISYKIREHSLQKLPYQLVVGDKEMQAATVAVRTRSGEDLGAMSLDAFT
                     ARLGSEMRAEAKAQ"
     gene            14263..14877
                     /gene="infC"
                     /locus_tag="VIG70_18470"
     CDS             14263..14877
                     /gene="infC"
                     /locus_tag="VIG70_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY8252582.1"
                     /translation="MALERSQRINREITAPEVRLVAENGDQLGIKPVAEALRLAEEQN
                     VDLVEIAPLANPPVCKLMDYGKFRYREQKKAHEAKLKQKQIQVKEIKFRPGTDEGDYK
                     IKLGKLIQFLEEGDKAKVTLRFRGREMAHQEFGVRLLERVRKDLDAVGLVEQFPKLEG
                     RQMVMVLAPKKKGPAPKVAKPKEPKEPKEPKAAKPAAPPKRQAA"
     gene            14982..15182
                     /gene="rpmI"
                     /locus_tag="VIG70_18475"
     CDS             14982..15182
                     /gene="rpmI"
                     /locus_tag="VIG70_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY8252583.1"
                     /translation="MPKMKSKRGAAKRFKVRGSGSIKRAGAYKRHILTKKSTKRKRQM
                     RRRSGVDHTNTAAIRSMLPYSS"
     gene            15192..15551
                     /gene="rplT"
                     /locus_tag="VIG70_18480"
     CDS             15192..15551
                     /gene="rplT"
                     /locus_tag="VIG70_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY8252584.1"
                     /translation="MPRVKRGVTARARHKKVFKQAKGFRGRRKNVYRISKQAVMKAGQ
                     YAYRDRRQKKRQFRALWIARINAAVRELGMSYSVFMAGLKRANIEIDRKVLADLAVMD
                     KPAFDKIAAQVKASIAH"
     gene            15567..16568
                     /gene="pheS"
                     /locus_tag="VIG70_18485"
     CDS             15567..16568
                     /gene="pheS"
                     /locus_tag="VIG70_18485"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY8252585.1"
                     /translation="MEQLGVIVSRALGDFAAASDPASLENAKARYLGKAGELTALQST
                     LKSAPPEQRRELGAKFNDAKQKIEAGLEARRAALAEAKLTARLTEQSLDVTLPGRGRG
                     RGGVHPVIRTWQRIEEIWRSIGFEVADGPEIETDWFNFTALNSPENHPSRSMQDTFYV
                     DLKDANGLPLVLRTHTSPMQVRYARTHKPPIKVIAPGRTYRVDSDATHSPMFHQVEGL
                     WIDEHISFADLKGVYTEFLRRFFENDELEVRFRPSFFPFTEPSAEIDMKLKGRWLEIS
                     GSGQVHPDVVRNFGLDPERYIGFAFGSGLERLTMLRYGIDDLRLFFDGDLRFLEQFS"
     gene            16565..18940
                     /gene="pheT"
                     /locus_tag="VIG70_18490"
     CDS             16565..18940
                     /gene="pheT"
                     /locus_tag="VIG70_18490"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY8252586.1"
                     /translation="MNIPESWLRSFCDPALPGRELADKLTMAGIEVENYEPVGPQFSG
                     VVVAEVMAVEKHPNADKLTVCKVKAGKDTLQVVCGAPNVRAGMKAPLAKVGAKLPGLE
                     IKATSLRGVESQGMLCSARELGLSDDHSGLLEIEGKAGSDARAALGLDDHILTLKLTP
                     NRADCLSVLGVAREVAAVTRSKLKPLEIKAVPAKSKAKHAVKLANPEGCGRFAGRVIR
                     GVDASASTPSWMRERLERAGQRSISALVDVTNYVMLELGRPLHVYDLDKLRGTIVVRW
                     GRKGEKVLLLNEQEVEVDAQVLCITDDSGVIGLGGIMGGESTKADESTRNVFLESAFF
                     YPDAIAGRARRYNFGSDASHRFERGVDFENNVDGIERATKLIMDICGGEPGPTVDTVA
                     RLPRRKPVAMRVARAQKVIGVPVPAKEMASVFSRLGFESSLKKNAFTVKAPSYRFDLA
                     IEEDLIEEVARLYGFERIAAQPPRVAATMLKVPEAERSLHFFRERLAAADYREVINFS
                     FVEPGWETDFAGEANPIRLLNPIISQQSVMRTSLIGSLVAKIQYNHARKVPRIRVFEV
                     GRVFLRDAAAKDGPLSVAGLRQPIRIGAAAYGPALSEQWDTPARAVDLFDVKSDLGNV
                     LAPLAPRFEPAEHPAFHPGRSARVLVEGKPAGWIGELHPKWQQKYELPQAPVLFEVDA
                     APLQVVPLPRPGIPSDLPTVVRDISMLFALEAPVQAVFDAIEAEKPAIVRSVSLLSLF
                     RGQGVPDDRKSLAFRVVMQHTERTLTDAEADAARDALVALLGRKFAASLRT"
     gene            18965..19276
                     /locus_tag="VIG70_18495"
     CDS             18965..19276
                     /locus_tag="VIG70_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY8252587.1"
                     /translation="MTLTKAELADLLFEKVGLNKREAKDMVEAFFEEIRGALERGESV
                     KLSGFGNFQLRDKPQRPGRNPKTGEEIPISARRVVTFHASQKLKAMVEKARHGAKPQE
                     S"
     gene            19251..19661
                     /locus_tag="VIG70_18500"
     CDS             19251..19661
                     /locus_tag="VIG70_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8252588.1"
                     /translation="MEPSRRKAELPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEFTQ
                     LKPVKRRGNRRYYQHHEVLLIRRIRQLLYEEGFTISGARNRLDNARLESARIDAEEKS
                     EPPAPRELRVVPGAGVDVAAIKRELRAVLDQLQG"
     gene            19674..19750
                     /locus_tag="VIG70_18505"
     tRNA            19674..19750
                     /locus_tag="VIG70_18505"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19708..19710,aa:Pro,seq:ggg)
     gene            19835..20302
                     /locus_tag="VIG70_18510"
     CDS             19835..20302
                     /locus_tag="VIG70_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEY8252589.1"
                     /translation="MDTGISTKDRAAIAKGLSRLLADTYVVYLKTHNFHWNVEGPMFQ
                     TLHQMFMEQYTEAWNAIDLVAERIRSLGHYAPGTYREYLDLARVKETPGVPKAVQMVK
                     LLIEGQEAVVKTAREVLPLAEKADDQPTLDLLTQRMQVHEKNAWMLRSLLKKQ"
     gene            20384..20761
                     /locus_tag="VIG70_18515"
     CDS             20384..20761
                     /locus_tag="VIG70_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252590.1"
                     /translation="MHFNFQLAQDHLRVELFGRQTLEDTLQFVEALAAEARKHSALPI
                     LIWVRNSRPIFKVERYPLSERFRQLAAQKGVRVALLADSDEVRASNQYIEVLAGQQGA
                     SVRAFREESRALNWLTAQSQKER"
     gene            20806..21549
                     /gene="surE"
                     /locus_tag="VIG70_18520"
     CDS             20806..21549
                     /gene="surE"
                     /locus_tag="VIG70_18520"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY8252591.1"
                     /translation="MKILVSNDDGYFAPGITLLAEALGQLGEVTVVAPERDRSGASNS
                     LTLDRPLTVRRAPNGYFSVNGTPTDCVHIAVTGLLDFLPDIVVSGVNLGANMGDDTIY
                     SGTVAAAAEGYLLGIPSVAVSLTSKTGEHFQSAIGVAVQIVERFKRHPFAQPVLLNVN
                     VPDVPPESLRGIEVTRLGRRHKAEPVVKLQTPRGETAYWIGPAGGAADAGPGTDFHAV
                     ESQRVSVTPLRMDLTHNAQLTLAKDWMAA"
     gene            21573..22229
                     /locus_tag="VIG70_18525"
     CDS             21573..22229
                     /locus_tag="VIG70_18525"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY8252592.1"
                     /translation="MTSERTRKRMVERLRDQGIRDERVLAAMAAVPRHLFVEEALASR
                     AYEDTALPIGFGQTISQPYVVARMTEVLIENQSPKKLLEVGTGCGYQAAVLAHVFPEV
                     YSIERIKGLLERARGNLLSLRLKNLRLAHGDGYAGLEGAAPFQSIIVAAAAPRMPEAL
                     LRQLAPGGRMVLPLAQVAQGGKGGGGRQRLVLVERNGRGFLESELDAVRFVPMEAGKA
                     "
     gene            22226..23044
                     /locus_tag="VIG70_18530"
     CDS             22226..23044
                     /locus_tag="VIG70_18530"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8252593.1"
                     /translation="MKALAVVIALALIAGCAPQRRGAPVEDRRPPARAPAPKAPAAAV
                     PAAPPQAPASVPEGFYAVKKGDTLYSIALDHGHDYREVAQWNSLDDPTKLNVGQLLRV
                     KPPAQPAVVVGTGRAGSRIESRPLDGQKPVPAAAAAPAPAVASTSPREEPKLDVHPLT
                     FAWPVKGKVVAGFAEPRQKGIDIEGRSGDPVIASAAGRVTYVGSGIPGLGKLIVLKHD
                     QGYITVYAHNKDILVKEQQNVTRGQKIAELGEKLHFQIRKGAAAVDPLLYLPKS"
     gene            complement(23026..24006)
                     /gene="mltB"
                     /locus_tag="VIG70_18535"
     CDS             complement(23026..24006)
                     /gene="mltB"
                     /locus_tag="VIG70_18535"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEY8252594.1"
                     /translation="MIRLLIALLLCVPALAQASYAARPEVQAFVDDVAKRHGFVKSEL
                     KRVFAKAQRLDPVLEAIARPAERVRSWEEYRALLITERRIAEGVDFWKKYPRTLERAE
                     RKYGVAPQYVVAIIGVETFYGRNIGNWRVVDALTTLAFDYPPRAGFFRSELESYLLMT
                     REAGIDVFSVRGSYAGAIGIPQFMPSSARRYAVDFDGNGKIDLQKSRSDAIGSVANFL
                     KVHGWQRDADVAVGARVTGDAWRAYADGRFEPRHSMVELREAGVEFDSPEPAGAHGAL
                     VELGNTERPSDWRVGLRNFYVITRYNRSALYAAAVADLAKELKKAYDLGR"
     gene            complement(24003..25955)
                     /locus_tag="VIG70_18540"
     CDS             complement(24003..25955)
                     /locus_tag="VIG70_18540"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEY8252595.1"
                     /translation="MNHPLAPEQRSVGARDLVWLIASLVLVIAPHALRAPWWLTLLTL
                     CLFGWRLYFALNRAPLPSRWLVLGVAAVAMLGVWIEFRTLFGRQSGILLLMLFAGLKL
                     LETRTLRDAAIAVFLGYFLVITNFLYTQTIPTAAAMCAALFLLTATLIGFAAPQRALR
                     ANLRTAGLLLAHAAPAALALFLLFPRVQGPLWGLPQDAYTAMTGLSETMSPGTLSGLA
                     LSDSIAFRAEFQGAPPPQPVRYWRGPVLWDFDGRTWSIGPGFLVDFAPPRGGRGSYRY
                     EVVLEPHNRHWLFALETAASLPERARMSFDGQIVSGSPVRARMRYDVASVLAPEPQAK
                     ETAGTLRRALRLPQGFNPRASALAAQWRADSDDDAEVLGRAIAFLRQGQYVYTLEPPL
                     LGEDTVDDFLFSTKAGFCEHFSSAFVFLMRAAGVPSRVVTGYQGGEANPVDSIITVRQ
                     SDAHAWAEVFLPARGWVRVDPTAAAVPGRIESGMARAVPQASELPLLMRPQLEWLRGV
                     RDRWEAVAHKWNVWVLGYNPERQRDLLSSIGVRDADWRTLTAALFTFLGAMTLVLLAW
                     SLRRLARPDPVQKAWRRFCRKLAARGIERAPAEGPRDFAARAARALPAARRSILRIGA
                     LYIALRYGAHATQPGVARLRRWVRAF"
     gene            complement(25952..26914)
                     /locus_tag="VIG70_18545"
     CDS             complement(25952..26914)
                     /locus_tag="VIG70_18545"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8252596.1"
                     /translation="MIGRLLERNPRIANWLYGFTPPERGVVELVHRRVYIVPARLGWY
                     FGGTLLVLLVGSINYALSLGFALTFLLAGLGLVGMVHTARNLARIAASAGRAEPVFAG
                     ESAQFRLYLDGRAAFDRPAILARHLASGSQLVVDIAPRGVSEVILAVPAPRRGWLPLG
                     RVMLETRFPLGLFRAWSYIEPDARCLVYPRPERSPLPPYSGEAVAGALHAPTPGSDDF
                     AGLRGYQRSDSPRHIAWKAVARSEELLTKQFAGDAAAELWLDARLLPHSLTLEQTLSR
                     LAGWVLAADRGGAHYGLRLPGVEIAPARGDVHRAACLQALALYS"
     gene            complement(26911..27507)
                     /locus_tag="VIG70_18550"
     CDS             complement(26911..27507)
                     /locus_tag="VIG70_18550"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY8252597.1"
                     /translation="MIGRAAIVAIFLCPAPGLAGEQKYEPLADSVRQRLSRMVADRAP
                     AGMHFREALDGQRWLSEMDRRLEKRIPDRQQRLELLRTVQYEATRARLDPQLVLGVIE
                     VESGFRKYAVSRAGARGYMQVMPFWVKLIGQPSHNLFHLRANLAYGCVILRHYLDLEK
                     GDYWRALGRYNGSLGKPEYPNQVLAAWRGRWKYDGPTA"
     gene            complement(27504..29195)
                     /locus_tag="VIG70_18555"
     CDS             complement(27504..29195)
                     /locus_tag="VIG70_18555"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY8252598.1"
                     /translation="MRTSKFFISTLKEVPAEAEVVSHRLMLRASIIRRLSAGIYTWLP
                     LGMRVLQKVEAIVREEMNRAGAVELSMPVVQPGELWQESHRWQEYGPELLRFKDRHER
                     DFVIQPTSEEAITELARAELKSYRRLPVHFYQIQTKFRDERRPRFGIMRGREFIMKDG
                     YSFHADYESLEREYRNMHDTYTRIFTRLGLRFRPVAADTGAIGGTGSHEFHVLADSGE
                     DAIAFSPQSDYAANVELAEAFAPSKRSNPSEGMKKVATPGKATCEAVAELLKLPLQRT
                     VKSVAVMHESEFHLLLVRGDHALNEIKTAKVIGTFRFAGEKEIVAALGCKPGYIGPVG
                     VKVKLIADRTVAAMSDFVCGANEEGFHLTGVNWGRDLPEPQVADIRNVVAGDPSPDGK
                     GKLEIARGIEVGHIFQLRTKYSEAMKATFLGEDGTARPFEMGCYGIGVTRIVAAAIEQ
                     NHDARGIVFPAPMAPFQACLVPIGYHKSAQVREAAERLYGELRAAGIDVLMDDRDERP
                     GVLFADMELIGIPHRVVLSERGLGAGNAEYKGRRDEKPQDLPLQDVVAFLKDRLG"
     gene            29276..29773
                     /locus_tag="VIG70_18560"
                     /pseudo
     CDS             29276..29773
                     /locus_tag="VIG70_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
     gene            29832..30455
                     /locus_tag="VIG70_18565"
     CDS             29832..30455
                     /locus_tag="VIG70_18565"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8252599.1"
                     /translation="MDREYDALSIRELGAGAGLRRPTQAPAARVSVESPALDVMTDLR
                     RSTPAAIRPQAPLAGANQFMIARGVRLLLVVDEREDVLGVISATDILGERPMRVATER
                     GMRRDELTVADIMIPAEQVEVIALPDVAAARVGHVLETLRRAGRQHALVVDFDDIPSS
                     RPLTPPARRAMVRGIFSLSQIARQLGLSVATGGEVARTFSEIEAAIR"
     gene            30471..31046
                     /locus_tag="VIG70_18570"
     CDS             30471..31046
                     /locus_tag="VIG70_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEY8252600.1"
                     /translation="MIERDSLLSLEAYAKERNAFRAQVMEHKKRRTVHLGGHLTLLFE
                     DELTVRYQVQEMLRIERIFEEEGIRHELDAYNPLVPDGGNWKATMLLEYPDEAERRRM
                     LGALKGIERRVWVQVAGAGRVYAIADEDLERENDEKTSSVHFLRFELEGPMRERLRQG
                     SAVTVGVDHPHYAAAVELAPEQRAALASDLT"
     gene            31043..31588
                     /locus_tag="VIG70_18575"
     CDS             31043..31588
                     /locus_tag="VIG70_18575"
                     /inference="COORDINATES: protein motif:HMM:NF020331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEY8252601.1"
                     /translation="MRKWLIAVFALAACSGPTEKSDWELQNEARLAPPEETVALPPYP
                     AEQRLIEFSVAATSEFRFFIDAASISVGKEGVVRYTLVARNPAAADNVSFEGMRCTAA
                     EVRTYAVGRDGGWMRRTGGWRSIEGTSVQRWYSTLYREYFCPQAQAITSAQEGILSLR
                     RGGASITRSLTEDIPRGGGAR"
     gene            complement(31585..32700)
                     /gene="proB"
                     /locus_tag="VIG70_18580"
     CDS             complement(31585..32700)
                     /gene="proB"
                     /locus_tag="VIG70_18580"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY8252602.1"
                     /translation="MTSPRAHLANAKRIVVKVGSALLAEKEGDAFARFADQVAQLRAK
                     GASVVVVSSGAIALGLAVLGYKERPKDLAGLQACAAAGQSKLMAKWDAAFSSHGTTVA
                     QVLLTHDDLRDRRRYLNARHALLALVQRGCVPIVNENDTVAVEEIKLGDNDTLAALVT
                     NLIEADALVILTDQAGLFDADPRKHQQAKIIQEANASDSRLESLAGGAGSALGKGGML
                     TKVLAARRAARSGAHTVIASGAEPDVLLRLARGERIGTLLTAQTVPLAARKQWLADHL
                     TVSGRVRLDAGAVKALVRDGKSLLPIGVTEVAGEFQRGEVVACLDPAGREIARGLANY
                     PSAEARQIIRKPSGEIEAILGYVGEPELIHRDNLVLL"
     gene            complement(32708..>33228)
                     /locus_tag="VIG70_18585"
     CDS             complement(32708..>33228)
                     /locus_tag="VIG70_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252603.1"
                     /translation="SIPDQSGAAAAEGGSVPQPPQPQLNDAGVLEDVEPPAEKKTRIR
                     DRLGKWRTRAKPAGGSGELWVDVDSGVITMVRFEGALAVGDGAAPSVLKVKVDQSFHD
                     IGKDQKIAMPKDAIEEISRQKMPVRPRELLEEEGIVKPLPKDGGPSSSGPGSKPKPAA
                     ASAAPGELPDDE"
BASE COUNT         5594 a        11097 c        11396 g         5141 t
ORIGIN      
        1 cggccgcctg tgcgacctcg ccgcgcgcta tcgcctggcc gccaacgtcg agccgatgcc
       61 ctgggtcgag gtttccagcg tctcgaagac gaagcgggtc ctcgatcagg cggcacggaa
      121 gaattccgcg ctgctggtgg atgcaattca ttttttcagg gaagaaaaca agcttgagca
      181 gctgcaagaa atccggctga attacatgca gctttgcgac gcgcggccgg agcggccggc
      241 ggacatgcag gagatcatcc ggcaggcgcg ggccgaccga ttgtttcccg gcgagggcgg
      301 gctcgacctg caagggctga tgcgcgcgct gccgcgcgag cttccgatca gcgtagaaat
      361 tccgtacgcg aagccgatgg cgccgctcga gcgggcacag cgtgcgcttg ccgctacgcg
      421 attacttctg tctcgggttg cgtagccctt cgtccggcgc gcgataggtg ccggagcgga
      481 tcgcgcgcac catgttgacg aggcttttcg ccgcgttctc gacttgcacg aaggtctcgg
      541 actgcgagtc gagcttgtcg tggctcgtcg cgtagggctc ccagtagccg atcagggtgt
      601 ccaccgccgc cgacacgccg gcctggatca tgccgatgct cgcgagccac tccaccagcg
      661 cttcgcgcag gccctcgtgg ccctgcgaat cgccatgtgc gaccaccgcg aacgcgcgcc
      721 cggcgaggtg gcgcgggtag ggccatcccg cgagctccag cgccttggcg gccgcgggat
      781 ccttgccgcg cgtcgtcgtg ggatcgggat tgcctccgtc tgcgcacacc agacgatcca
      841 tcatcagctt gaggctcgcg ggcgcctggt accagtgcac cgggcacagc acgaacacgc
      901 cgtgcgccgc gacccagcgt ggatagagct cggccatcca gtcgttggtc tggcccatcg
      961 cgtggttcgg gtagcagctg cacggccagt ggcacagcgc catcgaggtg gagacgcagg
     1021 ccttgcacgg atggatcacc ttccacggct cgtcggcgag ctgggacagc tcgaggaaat
     1081 cgacctcgaa tccggcctgc gcttcgatcg tgcgccgcgc gcgatcggcg aggcggcgcg
     1141 tcttcgagac ttcgcccggg caggtgtgct cgctgcgcgt cgcgccgctg acgacgagga
     1201 tgcgggaggc cgaggcggga tcggtttgcc gtttcgaagc ttgaagaatc gcggctcgtg
     1261 cttgcagcca ctcgaccgga agcttgaaac caggatcgga aaactccgac cccgcgggct
     1321 gcgagcgcgg cgacttgcgg tacttgatgt agccgtccca ggcgacctcg cacaccttct
     1381 cgagctccgc cttgacggcg tcgaatgccg ggtcgtagaa cctctcgaag aaccggctgc
     1441 ggaactgctc gcgcgtcagg ggaacgtact gcgtcatctt gcgcacggtg gtcatgaggg
     1501 atcctgtctg tagaaaagcc tgagctggtc gtagacgtcg ggatagcggc gtttggtttc
     1561 cgccggctgc tcgaagaacg cctcgctgat caccgcgaag aactccgacg ggtgctcggc
     1621 ggcgtaagga tcgagccaag tctccttgtc gcgctcgacc gcatcgcaga agccctcgta
     1681 cgcttgcgcg aaagccgcct gccacgcggc gcgatccatc ccggcgtgca gcggcggcag
     1741 gccgtcagcg gcgccgttca gcatgtcgag cttgtgggcg aactcgtgga tcaccacgtt
     1801 gatctcggga tcgttcgcgg tggcatccca ggaaaggacc accggcccgc cgggcatcgc
     1861 ttcgcccgcg agctcgtcgt cccactcgtg caccacgccg tgctcgtcgg tctcctcgcg
     1921 ccgcacgcgg aagtctcccg ggtagacgac gatgccggtg aagccggcat accagtccag
     1981 gccgagctcg aggatcggca ggcaggcttg cgccgcgatg gagatgcgca tcgcgtcggt
     2041 cacttccagg ccatgcgcgc cggtgaactg cttttcggcg aggaacagga gcgcgagctc
     2101 gcgcagcttc tcgctcttcg gaatgaaggg caggcccgtc gtggcctcgt tccagagcgc
     2161 ttcgtcgatg cggtgccggt cgagcacgcg ccggcggcgc caattcgtca gccagcccat
     2221 gcccgtaaaa tacccgcatg gtttcagtag tccaactgca tccgcaatcg ggtgccgccg
     2281 cgctcggccc gctcgcagac ggcctggcgg cgcccgagcg tgctctcatc ggccgtgcgc
     2341 tcgagttcgc cgagccgctg tacgccgggc aggtgctctc gaccggcgag ccctgctgga
     2401 gccatgcgct cggcctggca gcaagccttg ccgcgatcgg gctcgacgcg ccgggacggg
     2461 cagcaggcgt gctgttcgcc gcgccgaaat acctcgatgg aaccgagaag ctgggcgagg
     2521 cgttcggcgc cgagatcgcg gcgctggcgg caggcgtgga gaagctctac cagttgcggg
     2581 tggccacccg tggcacaccg agcgagcaaa acgaggtgct gcgcaagatg gtcctcggca
     2641 tggtcgagga cgtgcgggtg gtcctcgtcc ggctggcgag ccggacgcag acgctgaggt
     2701 tctttgccaa gaatccgtcg cccgagaggg cgtcctatgc ccgggagacc ctcgacatct
     2761 acgcgcccct cgccaaccgg ctgggcgtct ggcagctcaa atgggagctc gaggacctgt
     2821 cgttccgcta cctcgagccc gagctctaca agcgcatcgc gcagatgctc gacgaaaagc
     2881 gcctcgagcg cgagcaatac atcgccaatg cccttgcttt gctgaggacc gagctgaagg
     2941 ccatcggttt ggacagtaat gtcagcggac ggcccaagca catctactcc atctggaaca
     3001 agatgcgctc gaaagagctc gacttctccg aggtctacga cgtgcgcgcg ctgcgcgtga
     3061 tcgttcccga gatcaaggac tgctacgccg cgctcggcgt ggtgcacaac ctgtggcagc
     3121 cgatcccgaa ggagttcgac gactacattt cgcgccccaa gggcaacctt taccagtcgc
     3181 tgcacaccgc ggtgctcggg cccggcggca agacgctcga agtgcagatc cgcaccgagg
     3241 agatgcacaa gcaggcggag tacggcgttg ccgcgcactg gcagtacaag gaaaaggcca
     3301 aggcttcgaa gcaattcgag cagaaggtcg cctggctgcg gcagctgctg gcatggcgcg
     3361 atgaagttcc aatcaagcgc ccggagctcg aagacacgat ctacgtcctg acgccgcaag
     3421 gcaaggtgat cgacctgccg gcgggcgcga cgccgatcga cttcgcgtac gcgctgcaca
     3481 gcgacctcgg ccaccgctgc cgcggcgcgc gcgtcgacgg ccacatcgtg aagctcgata
     3541 cgccgctcgc gagcggccag cgcgtcgaga tcgtcgccgc gaagagcggc ggcccatcgc
     3601 gcgactggct caacccggag cgcggcttcg tgaagagcgt ccgctcgcgg cacaagatcc
     3661 ggcagtggtt caacgccaag gcgctcgccg agaccgtggc cgccggccgc gcagtggtcg
     3721 agaaggaact gcgccgcgaa ggcgccacgc aggcgaatct ggaggggttg gcggcgaagc
     3781 tcggctttgc caagcccgac gagatgttcg ccgcggtggc gcgcgacgag gtcaacctgc
     3841 gccagctgca ggtcgctctg aagggcgcgg cgccgcccgt ctcggcgcca gagctgaagc
     3901 gggcgaaaaa accgccgagc gcaaaggcat cgatgctcgt ggtcgggatg ggcgggctcg
     3961 cgacccagct cgcgcgctgc tgcaagccgg tgccacccga cccgatccgc ggcttcgtga
     4021 cccgcggcaa gggcgtgtcg atccaccgcg agagctgcga cggcctgaag cggctggcgg
     4081 aacgccatcc cgagcgcgtg atggagacgg actggggcaa aagcgatgcg gcttacgcgg
     4141 tcgacatgac cgtgaccgcc acggaccggc gcggcctgct gcgcgacatc ggcgatgccc
     4201 tcgcgcgcga gaagatcaac ctcaccgcgg tgcgcaccca gacccgcgac gatctcgcct
     4261 tcatgcgctt cactttcgac gtcgccgact cggggcagct caagcgcgcg ctggtcctgg
     4321 tgcgcgggct gaagggcgtg atccgcgtag cccggggatg aacccaaaaa cgagattggc
     4381 cgtcgtggct acaatagccg cacgcagtcc aacaagggag gtaccaaaat gaatcctgct
     4441 gcgcaatctc aagcaatgct catcggtcgg atcctgctcg cggcggtctt cctggtcgcc
     4501 ggcgcgcgca agctgatggg ggtggcggcg agcgccggct acttggccaa gctcggcttt
     4561 cccgcgcccg agattatggt gtgggttgct atcgtgatcg agatcggcgg cgccgtgctc
     4621 ctgatcaccg gctggcacgc acgccgggcg gcgtggctgc tgatcctgtt cgtggcgatc
     4681 gccaccgcga tggctcaccg cttctgggag ttcgatgccg cgcagtacaa caaccagctg
     4741 aaccacttcc tgaagaacgc ggcgatcatc ggtggcctgc tgtacgtcgc gatgctgggc
     4801 gcgggcgcgc tgagcctcgg cgccagatcc cggccttcat aggcttcatg aaaggatgat
     4861 tagcagccag accacgccag cgttgacgag cgagagcatc accgcggcgc tgccgatgtc
     4921 cttggcgcgc ttcgccaggc ggtggttctc gagcgagatg cggtcgacgg tcgcctcgac
     4981 tgcggcgttc accagctcga cgatcatcgc aaggatcacg ctgccgatca tcagcgcgcg
     5041 ctcggcgccg gtgtcgccga gccagaaagc cgccggcacg gcgaccagac agagcgctgc
     5101 gagctcgcgg aacgcggact cgtagcgcgc ggcggccgcg agcccggcaa acgaatagcg
     5161 cgtggcgttc aggacccggc gcagcccggt ctttcccttg tgagggctct cttctacgtg
     5221 ggaatcccga gcgtgaatag cagctcctcc tgcgcggaac ggcgccggcc gcggcgcggc
     5281 ttcacccgct cataggcgcc ctcggcgctc atctcccaga cctgggagct gtcgtcgagg
     5341 tagggctgca ggccctcgcg gatcacgcgg cgcttgagcg cggggtcgag caccgggaag
     5401 cacagctcga tgcggcggaa gaaattgcgg tccatccagt cggcgctcga gagatacacg
     5461 ttctcggcgc cgccgtcgtg gaagaagaag atgcgcgtgt gctcgaggaa gcggccgacc
     5521 accgagcgca cccggatatt ctcggaaagg ccctggatcc ccggcttgag cgcgcagacg
     5581 ccgcgcacga tgaggtcgat tttaaccccc gccgcggacg cggcgtacag ctcctcgatg
     5641 atctgcggct cgagcagcgc gttcatcttg gcgatgatgc gcgcgggctt gccctcggcg
     5701 gcctgcgcgg cctcgttgcg gatcgcggcg acgatgcgct tgtgcagcgt gaacggcgat
     5761 tgccataggt gcttcatcct gccggcgcgg cccagcccgg tgagctgctt gaacacttcg
     5821 gcgacgtcgg cgcacatctc ctcgttggcg gtgagcaggc cgaagtccgt atagaggcgc
     5881 gcggtgcgcg ggtggtagtt gccggtagcg aggtgtgcgt agcggcgcag gacgcgtgca
     5941 ccgccgtcgc cgccgtcctc gcgccgcacg acgagcgcca tcttcgcgtg ggtcttgtgc
     6001 ccgaccacgc cgtagaccac gtgcgcgccg acttcctcga gccgcgccgc ccagttgagg
     6061 ttggcctcct cgtcgaagcg cgccatcagc tcgaccacca ccgtgacttc cttgcccttg
     6121 cgcgcggcct cgaccaggat ctccatcagc tccgaatcgg tcccggtgcg gtacacggtc
     6181 tgcttgatcg cgatcacctg cgggtcgttg gccgtggtgc ggatgaaatc gatcaccggc
     6241 aggaacgact ggaacgggtg atggagcagc acgtcctgct tcttgagcac ttcgaagatg
     6301 tcgccggtat cgagcgcggg cggcaggccg ggctggaaca gcgggtattt caggtcgggc
     6361 cgcgccacct ggtcgggcac ctcgcgcaac cggtacaggt tgaccggccc ttctacgcgg
     6421 tagaggtcgg cctcgcgcag cccgaactgt tgcagcagga actccaccat gcgctgcgag
     6481 cagttgtcgg ccacctccag gcgcaccgcg tcgccgaagt ggcggtggaa gagctcgccc
     6541 tgcagcgcgg tatgcaggtt cttgatctcc tcctcgtcca cgaacaagtc ggaattgcgc
     6601 gtgacgcgga actggtagca gccctgcacg ttcatgcccg agaacagctc gtccatgtgc
     6661 gcgtgcagga tcgacgacag gaacacgaag gcgtactcgg cgtcgccggc gatcgcctgc
     6721 ggcaggcgga tcaggcgcgg cagcgcgcgc ggcgcctgca cgatcgccgc gcccgagctg
     6781 cggccgaagg cgtcgcggcc ctcgagctcg agcgcaaagt tgaggctctt gttgaacacg
     6841 cgcgggaaag gatgcgcggg gtcgaggccg atcggcgtca gcaccggcag gagctcgcgg
     6901 aagaaatagt cgcggatcca ctggcgctgc gccgcgttcc agtcctcgcg cttcgggaac
     6961 acgatgcgct gcgcggcgag cgccggcagc acggtctggt tgaggagctg gtattgctcg
     7021 gcgacgagcg cgtgcgcttc gcggctcacc gactcgaaga cctcggcggg cgcgccgccg
     7081 tcgggccccg gctcgggcag gccgagcttg atctgctcct tgaggcccgc gacgcggatc
     7141 tcgaagaact cgtccaggtt ggacgagacg atggtgatga agcgcagccg ctcgagcagc
     7201 ggcacggtgt cgtccgcggc ctgcgccagc acccggcggt tgaacgccag gatgccgagc
     7261 tcgcggttga ggaactgggc ggggacgggg actctgcggt tcatcggctg cccggtgggg
     7321 accgtggaag cggggaccgg aagagccatt gtaatggccg tgtccgccgg agaaatgacg
     7381 cggtccggcg ttttgcctag tccttcggat tccatcctgc accgcccctc gaccttcccc
     7441 gtcacaaatt ctgcacgcga ggcggtcgat tcttgatgac tgtttcatga ccgcggccaa
     7501 tcccctgatg gagcgcagaa atattccgga catgcagagc cgctagtgtc gctcggatga
     7561 cgacagacca tacttcgaag gatttcgacc acgcgctgga ccgcgcgcgc gagctggtga
     7621 gcctgatggc cgtgcgcgtc gagcgccagc tggccgacgc gatcgagtgc ctgcagaccg
     7681 ggtcgcccgc gctgatcgcg cagatcctgc gccacgaagc cgaagtgaac ggtctcgagc
     7741 gggcggtgga cgaggtcggc ggccagatca tcgcgcgccg ccatcccgcg gcgcgcgacc
     7801 tgcgcctggt gatgtcgctg ctcggcgcca ctaccgacct tgagcgcgtc ggcgacgagg
     7861 ccaagaagat ctcgctctac gcccgcagca tcttcggcgc cgggcggcag gtgctgccgc
     7921 gcgtgctgga gctcaggccg atgggggagc tggtgcacga catgctgcgc caggctacgc
     7981 gcgcgatgga agcgctcgac cccaacgccg cggcggcgct ggtgcgccgc gacctcgacg
     8041 tgaacgaacg cttccgcgcc gtgctggcga ggcttgccgg cttcatggtg gaggatccgc
     8101 gcagcatctc ggcctgcctc gacatcctgt tcgtcgccaa gtcgttcgag cgcatcggcg
     8161 accacgccaa gaatctcggc gagcacgtgg tctacgcgac gatcggaaaa gacgtccgcc
     8221 acgccacggc gcaggagatc gagcaagtcg tgcgcagtta gtttctgcta acttagccgg
     8281 atggcccggc acgacaccct tgccgccgtc gacctcggct cgaacagctt tcacctcgag
     8341 atcgggcgcg tggtcgacgg gcagatctac ccgctcgacg cggtgcgcga ggtggtgcgc
     8401 ctcggcggcg gcctcaccgc ggagaagcgc atcgatcgcg ccacccaggc tacggcgctc
     8461 gaggcgatcg caaagttcgg cgagcgcctg cgcggctttc cgcgcccggc ggtgcgcgcg
     8521 gtgggcacca acaccttgcg cgtggcgaag aacgcgccgc agttcctgcg cgaggcgcgc
     8581 gccgcgctcg gcttcccgat cgaggtgatc tccggacgcg aggaggcgcg cctgatctat
     8641 cttggcgtcg cgcattcgct gcccgcctcg gcgcagaagc gcctggtggt cgacgtcggc
     8701 ggcggctcga ccgagttcat catcggcacg ggctacgacg cgctgctcac cgagtcgctc
     8761 tacatgggct gcgtgagcta cagcctgaaa tactttcccg aaggcaaggt cgagaaatcc
     8821 ggcatgaagg cggccgagct cgccgcgcgc caggagcttg ccgccctggt caaggaatac
     8881 cgggacaccg gctgggagga ggccgtggcc tcctccggca ccgcgcgctc gatcgagaac
     8941 atcctgcgcg agaacgagct cgccgccgag gggctgacgc gcgaggggct cgatcggctg
     9001 agaagcctgc tgatcaaggc tgaaaaggcc gaccctgatc gcatccgcgg cctgcgcccg
     9061 aatcgcgcgc cggtgcttcc gggcggcctc gcgatcatga tcgccgtgtt cgacgccctc
     9121 ggcctcgaat cgatgaaggt gtccgagggc gcgctgcgcc acggcgtgct ctacgatctg
     9181 ctcggccgcg tgcagcaccg cgacatgcgc gcggccacgg tcgcgcagtt catgcgccgc
     9241 taccacatcg acgtccctca ggcggaccgg gtgcgcgccc ttgcgctcac catctacgat
     9301 gccctgaccc ccggcggcga ccgggaggac gatcccgatc gcgtgatgct ggaatgggcg
     9361 gcgcggctcg ccgaagtcgg cctgtcgatc gcgcacgccg cctaccacaa gcactcggcc
     9421 tacgtgctgt cgaatgccga catgccgggg ttctcgcgca tggagcaggc gcgcctcgcg
     9481 cgcctcgcgc tcgcgcaccg cggcaagctc ggcaaggtcc aggatgccgg tttcgaaagc
     9541 gcagactgga agctcgtgtt cggattgcgg ctggcgtcgc tcatcctgcg cagccgcagc
     9601 gacgccaagc ttccgttcct gcgcgttgcc gccgacgccg gcggcttcgc catcgacctg
     9661 cctcagtcct ggctcgagga gaacccgctg tcggccgacg cgctcgacgc cgaggcccgg
     9721 cactggaagg cggtcggcat gaagctcgag gtgaacggcc tctcggaccg gaaggtatcg
     9781 gtcctaaagc aggtcaggtg aaaccgtcgc gcgcaccacg acctggtcct tctcgtcgcg
     9841 cacgcggttg ctgatccacc acaagccgcc cttcttgatg ctgaaactgc cgcgcgcgcc
     9901 gagcagctcg gcgagcacgc gtcccaggtc gggctggtgg ccgacgatca cggtcgcgcc
     9961 cttgtaatcg ggccagccgc aggcctggat gatcgtcgaa ggcgaagcgc cgggcgccag
    10021 cgtgcggtcg gtcttgaccg gcacgccgag cgcttccgcg gtctgctgcg cgcgcgccgc
    10081 cgggctcgcg acgaccgcga agcgcgccgg caggcgctgc agcagccact gcgcgacgcg
    10141 ctcggcctgc ttgcggcctt tggcggtgag cttgcgctcg agatcgggaa cgccgtcttc
    10201 ggcttcggcg tgacgccaga taatgaggtc catcaaaaaa gtctacggta aaatcattgc
    10261 cattaggcgc gtagctcagc tggttagagc actaccttga catggtaggg gtcgttggtt
    10321 cgagtccaat cgcgcctacc agaatctcga ggatgacatg aagctgcgcg cgttcgcctt
    10381 ccggttcgtc ttcctctgcg ccttttccag cgccgcgctc gcgcaattcc cggcgcgggc
    10441 ggtcaccatc gtggtgccga tcccgcccgg cggggcgccc gacatcgccg cgcgcgtcat
    10501 cggccagaag ctctcggaga acatcggcca gccggtggtg gtggaaaaca aggtcggcgc
    10561 caacggcaac atcgcgaacg agcttgtcgc gcgcgcgcag cccgacggtt acacgctcgg
    10621 gctgctcgcc gacagccaga tcgccatcaa cccgcacctc tacaagatgc cgatcgatac
    10681 gctgcgcgac ctcacgccgg tgacgccggt cgcggtcaac cagttcgtgc tgacggtgaa
    10741 tccgtcgctg ccggtgcgca cgttccccga gttcatcgaa tacgcgaaga aggcgaaccc
    10801 gccgctcgcc tacgcttcgg gcggcaacgg cagccagcac cacctcacca tggagatgct
    10861 caagcagcgc gcgggcatca acctgctgca tgtgccctac aagggcggcg cgcctgccgc
    10921 gaccgcgacg gtcgcgggcg atacccaggc gatgtggtcg gggagctcga acgcgccgca
    10981 gatcaaggcc ggccggctgc gcccgctcgc ggtgtccggg gcgcaccgct cggcgcagta
    11041 tcccgagctg ccgacgatcg gcgagttcta tccaggcttg gagaactcga tctggctcgg
    11101 cctgttcggt ccggcgggaa ttccggaagc gacgctcggc aggttgcggg cggagctcaa
    11161 gcgcgtgctc gaatcgccgg acgtgaagga gaagctgaac gcggccggcg gtctggatcc
    11221 ctacatcgcg agccccgagg agttcgccgc gctcatcaag cgagactacg caaagtacgc
    11281 gaaagtggtg aaagacatcg gaataaagtt ggactagaag ccgtcctgaa ggcaagctcg
    11341 actagatctt ggcgcgctcg agcgtctcgg gcgcgaacag ctcctcaatc ttcagcgggc
    11401 gcgtggccac gccctgctcg aacgcgtatt gcacgaacgc ttccagcgtc ggacggttcg
    11461 gctcgatccc gtaaggccag gagtcctcgc ccgcggcctc gcgccagcgg cgcgcgtgct
    11521 cggcgagcca tggcatcggg atgtgcgaga tgccgatctc ggtgagccgc gcctggctca
    11581 cgcgcttcgc ctcctcgaac gccttgaaga ggttcatcgc gatccagcgg tcgcgctcgt
    11641 aggcttcgcg ccgcaggacg acgacgtgca tgatcgggaa gatgcgcgtt ttcctgaagt
    11701 actcgagctc caggccggcg tagtcggaaa acatgcgcgg ccctcccgga tcgcgcgccg
    11761 agatcaccgc gtcgagctcg ccgcccgcga gcatgccggc cagcgtcttg ccgggttgcg
    11821 gggtgatacg cactccctgc ggcagcttga gctcgacctt ctcttcgcgc ccgggttggt
    11881 gcacgcccgc ctgaatccat tcgatgcgcc gcaggtcgac gccgtactcg tgctgcagca
    11941 ggccgcggac gtagatcccc gcggtctggg cccattcggg gattccgacg cgcttgcctt
    12001 cgagctcctt cggggtcttg agcttcgtgt agatcgccga gtggcggaac acgcgcgacg
    12061 ggaacaccgg gagtgcgatg aggtccggcg acggcaggga caggatggac accaccttgc
    12121 cgaacgacat ctctgaggcg tcccattcgc ggaaccgcgt gaagcggtag aagatctcct
    12181 cgacggacag gtcgagcacc gtgaggtcga tcccctgagg gcggagatcg cgagcgtgat
    12241 cgtaggggct gatcgcgaga gagagcggca atttcaccgt gatagtatgc aagcactcga
    12301 gcgcaatgcc caacatccgt ctccccgacg gctcgtcgaa gtcgtttccc ggacccgtca
    12361 ccgtagccga gatcgcctcg tccatcggcg ccggcctggc gcgcgcagcg cttgcggcga
    12421 aggtcgacgg caagctggtt gacaccagct accgcgtcga gcgcgacgca acgatcagca
    12481 tcgtcaccga gcgtgacccg gaggggctcg agatcatccg ccactcgacc gcgcacctgc
    12541 tggcgcaggc ggtgaaggag ctgttccccg aagcgcaggt gacgatcggc ccggtgatcg
    12601 acaacggctt ctactacgac ttctcctaca agcggccttt tacgcccgag gacctggcgg
    12661 cgatcgaaaa gcggatggcg gagatctcga agcaggatct ccccgtccac cggcgtgaaa
    12721 tggcgcgcga cgaagccgtg gcctatttca agtcccttgg tgaaaactac aaggcggaga
    12781 tcatcgcctc gattcccggg aaggaaacga tctcgctcta cggccagggc aactggatcg
    12841 acctgtgccg cgggccgcac gtgccgtcga ccgggaagct caagaccttc aagctgatgc
    12901 gcgtggccgg ggcgtactgg cgcggcgact cgaagaacga gatgctccag cgcatctacg
    12961 gcacagcgtg ggcgaagaag gaggaccagg acgcctacct gaagatgctc gaggaggccg
    13021 agaagcgcga tcaccggcgc ctcggcacgc agctcgatct ctaccatatg caggaggagg
    13081 cgccgggcct ggtgttctgg catccgaagg gctggacgtt gtggcaggcg gtcgagcagt
    13141 acatgcgccg cgtgtaccgc gacaacggct accacgaggt gcgcgggccg cagatcctcg
    13201 acgtggagct ctggaagcga accgggcact gggacaactt ccgcgagaac atgttcttca
    13261 ccgcgtccga gaaccgcgac tacgcggtga agccgatgaa ctgcccgggg cacatcctga
    13321 tctacaacaa gggcgtacgc agctaccgcg agctgccgct gcgctatggc gagttcggct
    13381 cgtgccatcg caacgagccc tcgggcgcgc tgcacgggct gatgcgcgtg cgcggcttcg
    13441 tgcaggacga cgggcacatc ttttgcaccg aggagcagat cctgggcgag tgcgttgcct
    13501 ataccgccct attgcagaag gtgtatcgcg atttcggctt caccgacatc atctacaaag
    13561 tcgccacccg gccggcgaaa cgcatcggct cggacgagag ttgggatcgc gccgagcatg
    13621 ccttgaagac ttcacttgaa aagtcgggcg tgcaattctc cgtatccccc ggcgagggcg
    13681 cgttttacgg cccgaagatc gagtattcgc tgaaggacgg gatcggccgc gtctggcaat
    13741 gcggcacgat gcaggttgat ttcgccatgc ccgcgcggct cggcgccgag tacgtcgccg
    13801 aggacaacac gcgccgcacc ccggtgatgc tccaccgcgc aatcgtcggc tccttcgagc
    13861 gcttcatcgg catcctgatc gagcactacg cgggagcgtt ccctctgtgg ctcgcgccgc
    13921 accagctggt ggtgatgaac attaccgagc ggcaggccga gtacgccctt tccgtgaaaa
    13981 ataaactcgt gaatcaaggg tttagggcaa ttttggattt gcggaacgag aaaatttctt
    14041 ataagattcg cgagcatagc ctgcagaaac tgccctacca gctggtggtg ggagacaagg
    14101 agatgcaggc ggcaacggtg gccgtgcgca cgcgcagcgg cgaagacctc ggggcgatga
    14161 gcctggatgc cttcaccgcg cgcctggggt ccgaaatgcg ggccgaggcg aaagcgcagt
    14221 agcacggcca attttttcaa ctaacgaagg agcttttcac acatcgcact cgagagatcg
    14281 cagcgcatca accgggaaat caccgccccg gaggtgcgcc tggtggcgga aaacggtgac
    14341 cagctcggaa tcaagccggt agcagaggcg ctccgcctgg cggaggagca gaacgtggac
    14401 ctggtggaga tcgcgccgct cgcgaacccg ccggtctgca agctgatgga ctacggcaag
    14461 ttccgctacc gcgagcagaa gaaggcgcac gaggcgaagc tcaagcagaa gcagatccag
    14521 gtcaaggaaa tcaagttccg cccgggtacc gacgagggcg actacaagat caagctcggg
    14581 aagctgatcc agtttctcga ggaaggcgac aaggcgaagg tgacgctgcg cttccgcggc
    14641 cgcgagatgg cgcaccagga gttcggcgtg cggctgctgg agcgggtgag gaaggatctg
    14701 gacgcggtcg gcctggtcga gcagtttccc aagctcgagg gccggcagat ggtgatggtg
    14761 ctggcgccga agaagaaggg accggcgccg aaggttgcga agccgaagga accgaaagag
    14821 ccgaaagagc caaaggcagc aaagcccgca gcgcccccga agcgccaagc ggcatagcag
    14881 caaagcagtc actacaagtg atggccgtgg cagttgaagc gtccccgcgt aggcggggac
    14941 cgcccggcgt caagctaaaa gagagaagga gttcgttcgt catgccgaag atgaaatcca
    15001 agcgcggcgc cgccaagcgc ttcaaggtgc gcggcagcgg ctcgatcaag cgagccgggg
    15061 cctacaagcg ccacatcctc accaagaaga gcaccaagcg taagcgccag atgcgccgca
    15121 ggagcggcgt agaccatacc aacacggcgg ccatccgttc gatgctgccg tactcgtcct
    15181 aggagcgcgc catgccaagg gtcaagcgcg gagtcacggc gcgcgcgcgc cacaagaagg
    15241 tcttcaagca ggccaagggt tttcgcggcc gcaggaaaaa cgtctaccgg atctcgaagc
    15301 aggcggtgat gaaggcgggg cagtacgcct accgcgaccg ccgccagaaa aagcgccagt
    15361 tccgcgcgct ctggatcgcg cgcatcaacg ccgcggtgcg cgagctcggc atgagctata
    15421 gcgtgttcat ggcggggctc aagcgcgcca acatcgagat cgaccgcaag gtgctggccg
    15481 acctcgcggt catggacaag cccgcgttcg acaagatcgc agcgcaggtg aaggcaagca
    15541 tcgctcactg acgttgcaag acgtagatgg agcaactcgg ggtcatcgtc tcacgggcgc
    15601 tcggcgattt cgccgccgca tcggacccgg cttcgctcga gaacgccaag gcgcgctatc
    15661 tcggcaaggc cggcgagctg actgcgcttc agtcgacgct caagtcggct cctccggagc
    15721 agcggcggga gctgggcgcg aagttcaacg acgcgaagca gaagatcgag gccgggctcg
    15781 aggcgcgccg cgccgcgctc gccgaagcga agctcacggc gcggctcacc gagcagtcgc
    15841 tcgacgtcac gctgccggga cggggccgcg gccgcggcgg ggtgcacccc gtgatccgca
    15901 cctggcagcg catcgaggaa atctggcgct cgatcggctt cgaggtcgcc gacggcccgg
    15961 aaatcgagac cgactggttc aacttcaccg cgctgaacag cccggaaaat cacccctcgc
    16021 gctcgatgca ggacactttc tacgtcgacc tgaaggacgc caatgggctg ccgctggtgc
    16081 tgcgcacgca taccagcccg atgcaggtgc gctacgcgcg cacgcacaag ccgccgatca
    16141 aggtgatcgc gcccggtcgc acctaccgcg tcgactcgga cgcgacgcat tcgccgatgt
    16201 tccaccaggt cgagggattg tggatcgacg agcacatcag cttcgccgac ctgaagggcg
    16261 tctacaccga gttcctgcgg cgcttcttcg agaatgacga gctcgaggtc cgcttccggc
    16321 cctcgttctt cccgttcact gaaccctccg ccgaaattga catgaagttg aagggccggt
    16381 ggctcgagat ctccggctcg ggccaggtcc acccggacgt cgtgcgcaac ttcgggctcg
    16441 accccgagcg ctacatcggc ttcgccttcg gctccggtct cgagcgcctc accatgctcc
    16501 ggtacggcat cgacgacctg cgcctgtttt tcgatggcga cctgcgcttt ctcgagcaat
    16561 tttcatgaac atccccgaga gctggctgcg cagcttctgc gatcccgcgc ttccggggcg
    16621 cgagctcgcg gacaagctga ccatggccgg gatcgaggtc gagaactacg agccggttgg
    16681 cccgcaattc agcggggtcg tcgtcgcaga ggtcatggcg gtggagaagc acccgaatgc
    16741 cgacaagctc accgtctgca aggtgaaggc gggcaaggac acgctgcagg tcgtctgcgg
    16801 cgcaccgaac gtgcgcgccg ggatgaaagc gcctctcgcc aaggtcgggg cgaagcttcc
    16861 ggggctcgaa atcaaggcca cgtccctgag gggggtggag agccaaggca tgctgtgctc
    16921 ggcgcgcgag cttggcctct ccgacgatca ctccggactt ttggaaatag aaggcaaggc
    16981 cgggagcgac gcgcgcgctg cgctgggcct ggacgatcac atcctgacgc tcaagctgac
    17041 gccgaaccgg gccgactgcc tttcggtgct cggcgtcgcg cgcgaagtcg cggcggtgac
    17101 caggagcaag ctgaagccgc tcgagatcaa ggcagtgccc gcgaagagca aggcaaagca
    17161 tgcggtcaag ctcgcgaacc ccgagggctg cggcaggttt gccggccgcg ttattcgcgg
    17221 cgtcgatgcc tccgcgagca ctccgtcctg gatgcgcgag cgcctggagc gcgccgggca
    17281 gcgctcgatc tcggcgctgg tcgacgtcac caactacgtg atgcttgagc tcggccggcc
    17341 gctgcacgtc tacgacctcg acaagctgcg cgggacgatc gtggtgcgct ggggccgcaa
    17401 gggcgagaaa gtgctgctcc tgaacgagca ggaggtcgag gtcgacgcgc aggtgctgtg
    17461 catcaccgac gactcgggcg tcatcggcct gggcggcatc atgggcggcg agagcaccaa
    17521 ggccgacgag agcacgagga acgtgttcct cgagtcggcg ttcttctatc ccgacgccat
    17581 cgccgggcgc gcgcgccgct acaacttcgg cagcgacgcc tcgcatcgct tcgagcgcgg
    17641 cgtcgatttt gagaacaatg tcgacggcat cgagcgcgcg acgaagctga tcatggatat
    17701 ttgcggcggc gagcccggcc ccaccgtcga cacggtggcg cggctgccca ggcgcaagcc
    17761 cgtggcgatg cgcgtggcgc gggcgcagaa ggtgatcggc gttccggtgc ccgcgaagga
    17821 gatggcttcg gtgttttcgc gtctcgggtt cgagagttct ctcaagaaaa acgccttcac
    17881 ggtcaaggcg ccgtcctatc gcttcgacct cgcgatcgag gaggacctga tcgaggaggt
    17941 ggcgcggctc tacggcttcg agcgcatcgc cgcgcagccg ccgcgcgtcg cggcgacgat
    18001 gctgaaggtc ccggaggcgg aaaggagcct gcactttttc cgcgagcggc tcgccgcggc
    18061 cgactatcgc gaagtgatca acttcagctt tgtcgagccc ggctgggaaa ccgatttcgc
    18121 cggcgaagcg aacccgattc gcctgctgaa tcccatcatc agccagcagt cggtgatgcg
    18181 aacgagtctg atcgggtccc tggtcgcgaa gatccagtac aaccatgcac gcaaagttcc
    18241 gcgcatccgc gtcttcgagg tcggccgggt gttcctgcgc gacgccgccg ccaaggacgg
    18301 cccgctctcg gtcgccggcc tgcgccagcc gatccgcatc ggcgccgccg cctacggccc
    18361 ggccctgagc gagcagtggg atacgccggc gcgcgcggtt gacttgttcg acgtgaagtc
    18421 ggacctgggg aacgtgctcg cgccgcttgc cccccgcttc gaaccggcag agcacccggc
    18481 gttccacccc gggcgctccg cccgggtact ggtcgagggc aagccggccg gctggatcgg
    18541 cgagttgcac cccaagtggc agcagaagta cgagctgccc caggccccgg ttctgttcga
    18601 ggtggatgcc gcccccctcc aggtagtgcc gctaccccgc cccggcatcc cctccgacct
    18661 ccctaccgtg gtgcgcgaca tctctatgct tttcgccctg gaagcccccg tacaggccgt
    18721 ttttgatgcc attgaggccg aaaaaccggc gattgtgcgg tccgtgagcc tgctcagctt
    18781 gttccgagga cagggggttc ctgacgaccg gaaaagcctt gctttccggg tagttatgca
    18841 acatactgaa cgaaccctta cggatgcgga ggcagacgct gcccgcgacg cgctggtcgc
    18901 tctgctgggc cgcaaatttg ccgcaagtct ccgaacataa aagggaaatc aggggagcgc
    18961 gttgatgact ttgacaaagg ccgagctggc cgacctcttg ttcgagaagg tcgggctgaa
    19021 caagcgcgag gccaaagaca tggtcgaggc gtttttcgag gaaatccgtg gcgcgctgga
    19081 gcgcggggag agcgtcaagc tttccggctt cggcaacttc cagctgcgcg acaagccgca
    19141 acgccccggg cgcaacccca agaccggcga ggaaatacca ataagcgccc gccgggtcgt
    19201 caccttccat gccagccaga agctgaaggc gatggtcgaa aaggcgcgcc atggagccaa
    19261 gccgcaggaa agctgaactc ccgccgatcc cggcgaagcg ctacttcacc atcggtgagg
    19321 tgagcgagct ttgcggggtc aagccgcacg tgctgcgcta ttgggagcag gaattcacgc
    19381 agctcaagcc cgtcaagcgg cgcggcaacc gccgctacta ccagcaccac gaggtgctgc
    19441 tcatccgccg catccgccag ctcctgtacg aggaaggctt caccatcagc ggcgcgcgca
    19501 atcgtctgga caatgcgcgc ctggagagcg cgcggatcga tgccgaagaa aaatcggagc
    19561 cgccggcgcc gcgcgagctg cgcgtcgttc ccggggccgg agtcgacgtc gctgcgatca
    19621 agcgcgaatt gcgcgccgtc ctcgaccagc tgcaggggta aaatccgctt tctcggggcg
    19681 tagcgcagtc tggtagcgta ccggcatggg gtgccggtgg tcgtgagttc aaatctcacc
    19741 gccccgacca gtttcccctc ataggtttta tcaactcgcg ccatggcgaa ataccatttg
    19801 ctccggcgct tgggcgctcg ctaatctcct ctccatggac accggaatct cgaccaaaga
    19861 ccgcgcggcg atcgccaaag gcctgtcgcg cctgctcgcc gacacctacg tcgtctacct
    19921 gaagacgcac aacttccact ggaacgtcga aggcccgatg ttccagaccc tgcaccagat
    19981 gttcatggag cagtacaccg aggcgtggaa cgcgatcgac ctggtcgccg agcgcatccg
    20041 ctcgctcggt cactacgcgc ccggcacgta ccgcgaatac ctggacctcg cgcgcgtcaa
    20101 ggaaacgccg ggcgtgccca aagccgtgca gatggtgaag ctcctcatcg aggggcagga
    20161 ggcggtggtg aagaccgcgc gcgaagtgct cccgcttgcg gagaaagcgg acgaccagcc
    20221 gacgctggat ctgctcacgc agcgcatgca ggtgcacgaa aagaacgcct ggatgctgcg
    20281 ctcgctgctg aagaagcagt gatgcggtag ttcattccgc tacccgaatt tcacaagcgg
    20341 gcgctatagc cgagcgtgca agaatccccg taagaagacg gggatgcatt tcaatttcca
    20401 actggcgcag gatcatctgc gggtcgagct gttcggccgg cagacgctcg aggacacgct
    20461 gcagttcgtc gaggcgctcg ccgccgaggc gcgcaagcat tcggcgctcc ccatcctgat
    20521 ctgggtgcgc aattcgcgcc cgatcttcaa ggtcgagcgc tacccgctct ccgagcggtt
    20581 ccggcagctc gccgcccaga agggggtgcg cgtcgcgctg ctcgccgatt cggacgaggt
    20641 gcgcgcctcc aaccagtaca tcgaggtgct cgccggccag cagggcgcca gcgtgcgcgc
    20701 ctttcgcgag gaatcgcgcg cgctcaactg gctcacggct caatcccaga aagagcggta
    20761 ggcgggccgg acccggcgca cgacggttcc actagaatcg tccgcgtgaa aatcctggtt
    20821 tcgaacgacg acggctactt cgctccgggc atcacgctgc tcgccgaggc gctcggccag
    20881 ctcggggagg tgacggtggt tgcgccggag cgcgaccgct cgggggcctc gaactcgctg
    20941 acgctcgacc ggccgctcac ggtgcggcgc gcgccgaacg gctacttctc ggtcaacggc
    21001 acgccgaccg actgcgtgca tatcgcggtc accggcctgc tcgatttcct ccccgacatc
    21061 gtggtctccg gcgtcaacct cggcgccaac atgggggacg acaccatcta ttccggtacc
    21121 gtcgccgcag ccgccgaggg ctacctgctc ggcatcccgt cggtcgcggt ctcgctcacc
    21181 agcaagaccg gagagcattt ccagagcgcg atcggggtcg cggtgcagat cgtcgagcgg
    21241 ttcaagcgcc acccgtttgc ccagccggtg ctcctcaacg tgaacgtgcc tgacgttccg
    21301 ccggagagcc tgcgcggcat cgaggtcacg cggctcggcc ggcggcacaa ggccgagccg
    21361 gtggtcaagc tgcagacgcc gcggggcgag accgcctact ggatcggacc ggcgggcggc
    21421 gccgccgacg ccgggccggg cacggacttc catgcggtgg agtcgcaacg cgtgtcggtc
    21481 acgccgctgc gcatggacct tacgcacaac gcacagctca cgctcgccaa ggactggatg
    21541 gcggcgtgaa gaagaacgcc gcgggcgtcg gcatgacctc ggagcgcacg cgaaagcgca
    21601 tggtcgagcg cctgcgcgac cagggcatcc gcgacgagcg cgtgctcgcc gcgatggcgg
    21661 cggtgccgcg ccacctgttc gtcgaggaag cgcttgccag ccgcgcctac gaggacacgg
    21721 cgctgccgat cgggttcggc cagaccatct cgcagcctta cgtggttgcc cgaatgacgg
    21781 aagttctcat cgagaatcaa agtccaaaga agttgctcga agtgggcacg ggctgcggct
    21841 accaggccgc ggttctggcg cacgtgttcc cggaggtgta ctcgatcgag cgcatcaagg
    21901 gcctgctcga gcgcgcgcgc ggcaacctgc tctcgctgcg cctgaagaat ctgcggctgg
    21961 cgcacggcga cggctacgcc gggctggaag gcgctgcgcc gttccagtcg atcatcgtcg
    22021 ctgccgcggc gccgcgcatg cccgaagcgc tgctacgcca gctcgcgccc ggtggcagaa
    22081 tggtgctgcc gctggcgcag gtcgcgcagg gtggtaaagg cggcggcggc cgccagcggc
    22141 tggtgctcgt cgagcgcaac ggccgagggt ttctcgagtc ggagctcgac gcggtgcggt
    22201 tcgtgccgat ggaggcgggc aaggcgtgaa ggctttagcc gtggtcattg cgctggcgct
    22261 gattgctgga tgcgcgccgc agcgccgcgg ggcgccggtc gaggaccggc gtccgccggc
    22321 gcgcgcgccg gcgccgaagg ctccggcggc tgctgtacct gctgcgcctc cccaggcacc
    22381 tgcttcagtt cccgaaggct tctatgcggt gaagaagggc gacaccctat acagcatcgc
    22441 gcttgaccat ggccacgact accgggaagt cgcgcaatgg aattcgctcg acgacccgac
    22501 gaagctgaac gtcggccagt tgctgcgcgt aaagccgccg gcgcagccgg ccgtagtcgt
    22561 gggcacggga cgtgccggct cgcgcatcga atccaggccg ctcgacggac agaagcccgt
    22621 tcccgccgcc gccgccgcgc ctgcccctgc cgttgcctcg acctcgcctc gagaagagcc
    22681 caagctcgac gtgcatccgc tgaccttcgc ctggccggtg aagggcaagg tggttgccgg
    22741 cttcgccgag ccgcgacaaa agggcatcga catcgagggg aggtcgggcg acccggtgat
    22801 cgcctcggcg gcggggcgcg tgacctacgt cggcagcggc atccccggcc tgggcaagct
    22861 catcgtgctc aagcacgacc agggctacat cacggtgtac gcgcacaaca aggacatcct
    22921 ggtgaaggag cagcagaacg tgacgcgcgg ccagaagatc gccgagctcg gagagaagct
    22981 gcacttccag attcgcaagg gcgcggcggc cgtcgacccg ctgctctacc ttcccaagtc
    23041 gtaggctttc ttcagctcct tggcgaggtc ggcgacggcc gcggcgtaga gagcgctgcg
    23101 gttgtagcgc gtgatcacat agaagttgcg caagccgacg cgccagtcgc tcgggcgctc
    23161 ggtgttcccc agctcgacga gcgccccgtg ggcgcccgcg ggctcgggcg agtcgaattc
    23221 gaccccggcc tcgcgcagct cgaccatgga gtgcctgggc tcgaagcgcc cgtcggcgta
    23281 ggcgcgccag gcgtcgcccg tcacgcgcgc gccgaccgcc acgtcggcgt cgcgctgcca
    23341 gccgtggacc ttgaggaaat tggcgacgct gccgatcgcg tcggaccggc tcttctgcag
    23401 gtcgatcttg ccgttgccgt cgaagtcgac cgcgtagcgc cgggcgctcg acggcatgaa
    23461 ctgcgggatg ccgatggcgc cggcatagga gccgcgcacc gagaacacgt cgatgcccgc
    23521 ctcgcgcgtc atcaggagat agctctcgag ctcgctgcgg aagaagccgg cgcgcggcgg
    23581 gtagtcgaac gccagcgtgg tgagcgcgtc caccacgcgc cagttgccga tgttgcggcc
    23641 gtagaaggtc tcgacgccga tgatcgccac gacgtattgc ggcgcgacgc cgtacttgcg
    23701 ctcggcgcgc tcgagcgtgc gagggtattt cttccagaaa tccacgccct cggcgatgcg
    23761 ccgctcggtg atcaggaggg cgcggtactc ttcccaggag cgcacccgct ccgccggccg
    23821 cgcgatcgcc tccagcaccg ggtcgaggcg ctgcgccttg gcgaacacgc gtttgagctc
    23881 gcttttcacg aagccgtggc gctttgcgac gtcgtccacg aatgcctgaa cctcggggcg
    23941 ggcggcgtag gaggcctggg cgagggcagg cacgcacagc agcaacgcga tcaggaggcg
    24001 gatcaaaatg cccgcaccca tctgcgcagc cgcgcgaccc cgggttgcgt ggcgtgcgcg
    24061 ccgtagcgca gcgcgatgta aagcgcgccg atgcgcagga tgctgcgccg cgcggcgggc
    24121 aaggcgcgcg ccgcgcgcgc agcgaagtcg cgcgggccct ccgcgggggc gcgctcgatg
    24181 ccgcgcgcgg cgagcttgcg gcagaagcgg cgccacgcct tctgcaccgg atcgggacgc
    24241 gccagccgcc ggagcgacca ggcgagcaga acgagcgtca tcgcgccgag gaaggtgaag
    24301 agggccgcgg tgagcgtgcg ccagtcggcg tcgcgcacgc cgatgctgct caggaggtcg
    24361 cgctgacgct cggggttgta gccgagcacc cagacgttcc acttgtgcgc caccgcttcc
    24421 caccggtcgc gcacgccgcg cagccactcg agctgcgggc gcatcaggag cggcagctcg
    24481 ctcgcctggg gaacggcgcg cgccatgccc gactcgatgc gtcccggcac tgcggccgcg
    24541 gtcggatcga cgcgcaccca gccgcgcgcg ggaaggaaca cttccgccca ggcgtgggcg
    24601 tcggactggc gcacggtgat gatgctgtcg accggattcg cttcgccgcc ctggtagccg
    24661 gtcacgacgc gggacgggac tccggcggcg cgcatgagga acacgaacgc ggaggaaaag
    24721 tgctcgcaga accccgcctt ggtcgagaac aggaagtcgt cgaccgtgtc ttcgcccagg
    24781 agcggcggct cgagcgtgta cacgtattgg ccctgccgca ggaatgcgat cgctcgcccg
    24841 agcacttcgg cgtcgtcgtc ggagtccgcg cgccactgcg cggcgagcgc gctcgcgcgc
    24901 gggttgaagc cctgcggcag gcgcagcgcc cggcgcagcg tccccgcggt ctccttcgcc
    24961 tgcggctcgg gcgcgagcac cgaggcgacg tcgtagcgca tgcgcgcgcg caccggcgag
    25021 cccgacacga tctggccgtc gaagctcatg cgcgcgcgct cgggcaggct ggccgcggtc
    25081 tcgagcgcga acagccagtg ccggttgtgc ggctcgagca ccacctcgta gcggtagctg
    25141 ccgcgcccgc cgcgcggcgg ggcgaaatcg accaggaagc ccggcccgat gctccaggtg
    25201 cggccgtcga aatcccacag caccggaccg cgccagtagc gcaccggctg cggcgggggc
    25261 gcgccctgga actcggcgcg aaacgcgatc gaatccgaaa gcgcgaggcc cgacagggtt
    25321 cccgggctca tcgtctcgga cagcccggtc atcgcggtgt aggcgtcctg cggcaatccc
    25381 cacagcgggc cttgcacccg ggggaagagc aggaacagcg ccagcgccgc cggcgcggca
    25441 tgcgcgagca gcaggcccgc ggttctcagg ttcgcgcgca gcgcgcgctg cggcgccgca
    25501 aacccgatca gcgtggcggt gagcaggaac agcgccgcgc acatcgcggc cgcggtcggg
    25561 atggtctggg tatagaggaa attcgtgatc accaggaagt agccgaggaa cacggcgatc
    25621 gcggcgtcgc gcagcgtgcg cgtctcgagc agcttcaacc cggcgaacag catgaggagc
    25681 aggatccccg actggcggcc gaacagggtg cggaactcga tccacacgcc gagcatcgca
    25741 accgcggcca cgccgaggac cagccagcgc gaaggcagcg gcgcgcggtt gagcgcgaag
    25801 tacaggcgcc agccgaacag gcacagcgtg agcagcgtca gccaccacgg cgcgcgcagc
    25861 gcgtgcggcg cgatgaccag caccagcgag gcgatcagcc agacgagatc gcgcgccccg
    25921 acggatcgct gctccggagc aagcgggtgg ttcatgaata caaggcgaga gcctggaggc
    25981 atgcggcgcg gtgcacgtcg ccgcgcgccg gggcgatctc cacgccgggc aggcgcaggc
    26041 cgtagtgcgc gccgccgcga tcggctgcga gcacccagcc ggccagccgg gacaaggttt
    26101 gttccaaagt caaactatga ggaagcagtc gcgcgtcgag ccacagttcc gccgcggcgt
    26161 cgccggcgaa ctgcttggtg agcagctcct cgcttcgcgc caccgctttc cacgcgatgt
    26221 ggcgcggcga gtccgagcgc tggtagccgc gcaggccggc gaaatcgtcg ctgcccggag
    26281 tcggcgcgtg cagcgctccc gccacggcct cgccgctgta cggcggcagc ggcgagcgct
    26341 ccggtcgcgg atagacgagg cagcgcgcat cgggctcgat gtagctccag gcgcggaaca
    26401 ggcccagcgg gaaacgcgtc tcgagcatca cccgcccgag cggcagccag ccgcggcgcg
    26461 gcgccggcac cgccagtatc acttcgctca cgccgcgggg cgcaatgtcc accaccagct
    26521 gcgagcccga ggcgagatgg cgcgccagga tcgccggccg gtcgaacgcg gcgcggccgt
    26581 caagatagag gcggaactgc gccgactcgc ccgcgaacac cggctccgcg cgccctgcgc
    26641 tcgccgcgat gcgggcgagg ttgcgcgcgg tgtgcaccat tccgaccagg ccgaggcccg
    26701 cgagcaggaa ggtgagggcg aagccgagcg acagcgcgta gttgatcgag ccgacgagca
    26761 gcacgagcag cgtgccgccg aagtaccacc cgagccgcgc cggcacgatg tacacgcgcc
    26821 gatgcacgag ctcgaccacg ccgcgctcgg gcggcgtgaa gccgtagagc cagttggcga
    26881 tgcgcgggtt gcgctcgaga agtcggccga tcacgccgtg ggaccgtcgt atttccaccg
    26941 gccgcgccag gccgcgagca cctggttcgg atattccggc ttgccgaggc tgccgttgta
    27001 ccgcccgagc gcgcgccagt aatcgccttt ctcgaggtcg agatagtggc gcaggatgac
    27061 gcagccgtag gcgaggttgg cgcgcaggtg gaacagatta tggctcggct ggccgatcag
    27121 cttgacccag aacggcatca cctgcatgta gccgcgcgcc ccggcgcgcg acaccgcgta
    27181 cttgcgaaag ccgctctcga cctcgatcac gccgagcacg agctgcggat cgaggcgcgc
    27241 gcgcgtcgcc tcgtactgca ccgtgcgcag tagctcgagc cgctgctgcc ggtccggaat
    27301 gcgcttctcg aggcgccggt ccatctcgga cagccagcgc tggccatcca aggcctcgcg
    27361 gaaatgcatc cccgccggcg cccggtccgc caccatgcgc gagagccgct ggcgcacgct
    27421 gtcggcgagc ggctcgtatt tctgttctcc ggcaagacct ggcgccgggc acagaaatat
    27481 cgcgacaatc gcggcacgtc cgatcatccc agacggtctt tcaagaaggc cacgacgtcc
    27541 tgcagcggca ggtcctgcgg cttttcgtcg cgccgaccct tgtactcggc gttgccggcg
    27601 ccgaggccgc gctcggaaag caccacgcgg tgcgggatgc cgatgagctc catgtcggcg
    27661 aacagaacgc cggggcgctc gtcgcggtcg tccatcagca catcgatgcc tgcggcccgc
    27721 agctcgccgt acagccgctc ggcggcctcg cggacctggg cgctcttgtg atagccgatc
    27781 ggcaccaggc aggcctggaa cggcgccatc ggcgcgggga acacgatgcc gcgcgcgtcg
    27841 tggttctgct cgatcgccgc ggcgacgatg cgcgtcacgc cgatgccgta gcagcccatt
    27901 tcgaacggtc ttgcggttcc gtcctccccg aggaacgtgg ccttcatcgc ctcggagtat
    27961 ttggtccgga gctggaagat atgcccgacc tcgattccgc gcgcgatctc gagcttgccc
    28021 ttgccgtcgg ggctggggtc tccggcgacc acgttgcgaa tatccgccac ctgcggttcc
    28081 ggcaggtcgc ggccccagtt cacgcccgtg aggtggaaac cttcctcgtt ggcgccgcag
    28141 acgaagtcgc tcatcgccgc caccgtgcga tcggcgatca gcttcacttt caccccaacc
    28201 ggtccgatgt atcccggctt gcagccgagc gccgccacga tctctttctc gccggcgaag
    28261 cggaacgtgc cgatcacctt ggccgtcttg atctcgttca acgcatggtc gccgcgcacc
    28321 agcagcaggt ggaactcgct ctcgtgcatg acggcgaccg atttcacggt cctctggagc
    28381 ggcagcttga gaagctccgc aaccgcctca caggtcgcct tgcccggcgt ggcgaccttc
    28441 ttcatgcctt ccgaaggatt gctgcgcttg gacggcgcga acgcttccgc cagctcgacg
    28501 ttggccgcgt agtccgactg cggcgagaac gcgatcgcgt cttcgccgga gtcggcgaga
    28561 acgtggaact cgtgcgagcc ggtgccgccg atggcgccgg tatcggcggc gacggggcgg
    28621 aagcgcaggc cgagccgcgt gaagatgcgc gtgtaggtgt cgtgcatgtt gcggtactcg
    28681 cgctccaggc tctcgtagtc ggcgtggaac gagtagccgt ccttcatgat gaactcgcgc
    28741 ccgcgcatga tgccgaagcg cgggcggcgc tcgtcgcgga acttggtctg gatctggtag
    28801 aagtgcaccg gcaggcgccg gtagctcttc agctcggcgc gggcgagctc ggtaatcgcc
    28861 tcctccgagg tgggctggat cacgaagtcg cgctcgtgcc tgtccttgaa gcgcaacagc
    28921 tccggcccgt attcctgcca gcggtgcgac tcctgccaca gctcgcccgg ctgcaccacc
    28981 ggcatgctga gctcgaccgc gccggcgcgg ttcatctcct cgcgcacgat cgcttccact
    29041 ttctgcagca cgcgcatgcc gagcggcagc caggtgtaga tgcccgccga cagccgccgg
    29101 atgatgctgg cgcgcagcat cagccgatgg ctgaccactt cggcctcggc cggcacttcc
    29161 ttcagcgtgc tgatgaagaa cttcgaggtt ctcattggcg ccgcaccggc attgtcattg
    29221 tcgatgggct gtggaaaaat cgttcgaggc attctaactg aaggcggtcc ggcccatgct
    29281 ggacaaggaa ggctaccgtc cgaacgtcgg catcatcctc gccaaccaga ggaacgaggt
    29341 cttctggggc aagcgcgtga accagcacgc gtggcaattc ccgcagggcg gcatcaagcc
    29401 gggcgagacc ccgctgcagg ccatgtaccg cgagctcgag gaggagatcg ggctcgagcg
    29461 cgcgcacgtg cgcatcctcg gccgcacccg cgagtggctg cgctaccagg tgccggagcg
    29521 ctgggcgcgg cgccagcgcg aatcgaggga cgccaacccc ggcggaaagc agagcgctgg
    29581 gtaccgcggt cagaagcaga tctggtacct gctgcgcatg gtcgggcgcg actgcgacgt
    29641 gcggctgcgc gcgagcggcc atccggagtt tgacgcctgg cgctggcacg attactgggt
    29701 gccgctcgag gcggtgatcg agttcaagcg cgaggtgtac cgccaggcgc tgatcgagct
    29761 gcaccgcttt ctgctggccg accgccgcgg acgcgcggcg cgcgcgcagg ccgtgtcctg
    29821 atcggctgcc gatggaccgc gaatacgatg cgctgtcgat cagagagctg ggcgccggcg
    29881 cgggcttgcg gcggccgacg caggcgcccg cggccagggt ttcggtcgag tcgccggcgc
    29941 tcgacgtgat gaccgacctg cggcgcagca cgccggccgc catccggccg caggcgccgc
    30001 tcgccggcgc gaaccagttc atgatcgcgc gcggcgtgcg gctgctcctg gtggtcgacg
    30061 agcgcgagga cgtgctcggc gtgatcagcg cgaccgacat cctcggcgag cggccgatgc
    30121 gcgtcgccac cgagcgcggc atgcggcgcg acgagctcac ggtcgccgac atcatgattc
    30181 ccgccgagca ggtcgaggtg atcgccctcc cggacgtggc cgccgcgcgc gtcggtcacg
    30241 tgctcgagac gctgcgccgc gccggccgcc agcacgcgct ggtggtcgat ttcgacgaca
    30301 ttccctcgag ccgcccgctg accccgccgg cgcggcgcgc gatggtgcgc ggcatcttct
    30361 cgctgtcgca gatcgcgcgc cagctcggtc tttccgtggc gaccggcggc gaggtggcgc
    30421 gcacgttctc cgaaatcgaa gcggcgatca gatagtagtc tgacactccc atgatcgaac
    30481 gagactcgct cctgtccctg gaagcctacg cgaaggagcg caacgcgttt cgcgcccagg
    30541 tgatggagca caagaagcgc cgcacggtgc atctcggcgg ccatctcacg ctgctgttcg
    30601 aggacgagct caccgtccgc taccaggtgc aggagatgct gcgcatcgag cgcatcttcg
    30661 aggaggaggg catccggcac gagctcgacg cctacaaccc gctggtcccc gacggcggca
    30721 actggaaggc gaccatgctg ctcgaatacc cggacgaggc cgaacgccgg cgcatgctcg
    30781 gcgcgctgaa gggcatcgag cgccgggtct gggtgcaggt cgccggcgcg ggccgcgtgt
    30841 acgccattgc cgacgaggac ctcgagcggg agaacgacga gaagacctcg tcggtccatt
    30901 tcctgcgctt cgagctcgaa ggcccgatgc gtgagcggtt gcgtcaaggt tcggcggtca
    30961 cggtcggcgt cgatcacccg cactacgccg ccgcggtgga gcttgctccc gagcagcgcg
    31021 ccgcacttgc gagcgatctc acttgagaaa gtggctcatc gcggtcttcg cgctcgccgc
    31081 gtgcagcggc ccgaccgaga aaagcgactg ggagctccag aatgaagcgc ggctggcgcc
    31141 gccagaggag accgtggcgc tgccgccgta tcctgcggag cagcgcctga tcgagttctc
    31201 cgttgcggcg acgagcgagt tccgcttctt catcgacgcc gcttcgatca gcgtcggcaa
    31261 ggagggcgtg gttcggtaca cgctggtcgc gcgcaacccg gcggccgccg acaacgtgag
    31321 cttcgaaggc atgcgctgca ccgccgccga ggtgcgcacc tacgccgtgg ggcgcgacgg
    31381 cggctggatg cgtaggacgg gcgggtggcg ctcgatcgag ggaaccagcg tgcagcgctg
    31441 gtacagcacg ctctatcgcg agtatttctg ccctcaagcg caggcgatca cgagcgctca
    31501 ggaaggcatc ctgtcgctgc gtcgtggcgg agcctccatt acccgcagcc tgaccgaaga
    31561 catccctagg ggcggcggcg cccgctagag caacacgagg ttatctcggt gaatgagctc
    31621 cggctcgccg acgtagccga ggatcgcctc gatctcgcct gagggcttgc ggatgatctg
    31681 gcgcgcctcg gccgacgggt agttcgccag gccgcgcgcg atctcgcgcc ccgcgggatc
    31741 gaggcaggcg acgacctcgc cgcgctggaa ctcgcccgcg acctccgtga cgccgatcgg
    31801 aagcaggctc ttgccgtcgc gcaccagcgc cttgactgcc cccgcatcga gccgtacccg
    31861 gccgctcacc gtgaggtggt cggcgagcca ttgcttgcgc gccgcgagcg gcacggtctg
    31921 cgccgtgagc agggtgccga tgcgctcgcc gcgggcgagg cgcagcagca cgtcgggctc
    31981 ggcgcccgag gcgatcacgg tgtgcgcgcc gctgcgcgcc gcgcgccgcg cggccagcac
    32041 cttggtgagc atgccgccct tgccgagggc gctgccagcg ccgccggcga ggctttcgag
    32101 acgggaatcg ctcgcatttg cttcctgaat gatttttgct tgctggtgct tgcgcgggtc
    32161 ggcgtcgaac agcccggcct gatcggtgag gatcaccagc gcgtcggcct cgatcaggtt
    32221 ggtgacgagc gcggcgaggg tgtcgttgtc gccgagcttg atctcctcca cggcgaccgt
    32281 gtcgttctcg ttcacgatgg gcacgcagcc gcgctgcacg agcgccagga gcgcgtggcg
    32341 cgcgttcaga tagcggcgcc gatcgcgcag gtcgtcgtgc gtgagcagca cctgcgccac
    32401 cgtggtcccg tgggagctga aggccgcgtc ccacttggcc atgagcttgg actgccccgc
    32461 cgccgcgcac gcctgcagac cggcgaggtc cttggggcgc tccttgtagc cgagcacggc
    32521 gaggccgagg gcgatggcgc cgctcgagac gaccaccacc gacgcgccct tcgcgcgcag
    32581 ctgcgcgacc tggtccgcga agcgcgcgaa cgcgtcgccc tccttctccg cgagcagcgc
    32641 gctgccgacc ttgacgacga tccgcttggc gttcgcgagg tgcgcgcgcg gcgacgtcat
    32701 tgcgggctca ttcgtcgtcg gggagctcgc ccggggccgc cgatgctgct gctggcttcg
    32761 gcttgctgcc ggggccagac gaggatggac cgccgtcttt gggcaacggc ttcacgatgc
    32821 cctcctcctc gagcagctcg cgcggccgca ccggcatctt ctgccgcgag atctcctcga
    32881 tggcgtcctt gggcatggcg atcttctggt ccttgccgat gtcgtggaag gactggtcca
    32941 ccttgacctt gagcaccgac ggcgcggcgc catcgcccac ggcgagcgcg ccctcgaacc
    33001 gcaccatcgt gatcacgccg gagtcgacgt ccacccacag ctcgccgctg ccgccggcgg
    33061 gcttggcgcg cgtgcgccac ttgccgagcc ggtcgcggat gcgcgtcttc ttctcggccg
    33121 gcggctcgac gtcctcgagc acgccggcgt cgttcagctg gggctgcggc ggctgcggca
    33181 cggacccgcc ctcggccgcg gccgcgccgc tctggtccgg gatggaca
//
LOCUS       DATBSZ010000285        10809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2244090, whole genome shotgun sequence.
ACCESSION   DATBSZ010000285 DATBSZ010000000
VERSION     DATBSZ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2244090"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VIG70_18590"
     CDS             complement(<1..207)
                     /locus_tag="VIG70_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY8252604.1"
                     /translation="MRVFAMLLLAPALAAAQSLDPRAYSNLPVGLNFLLAGYAYSQGE
                     LAFDALPLQDARTRVHVLPVGYVRS"
     gene            complement(211..1398)
                     /locus_tag="VIG70_18595"
     CDS             complement(211..1398)
                     /locus_tag="VIG70_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252605.1"
                     /translation="MTKSAILQLPDFSLVLGGPLYQLFRRSHLTGDALEMLWRRILVI
                     TGVAWLPLLLLAALSGRTHDEAAGIPFLYDIEAHARFLIALPILIAAELVVHLRIRPV
                     VTQFVERRIVVPEELPKFQEAIDSTLRMRNSVIGEVTLIVVVYTLGLFVWRNQIALET
                     ASWYATPDGEGMHPTPAGYWYFFISLPIFQFILLRWYLRFFLWFSFLWRVSRLNLRLV
                     PIHPDKTAGLSFLGRSTNAFAPILFAQGAVLAGLLASQIFYAGQQLTDFKVEIGAFIA
                     FFVVVVFIPLTVFTPHLVRAKRQGMGEFGRLASRYTQEFEDKWVHADAPKDEALLGSA
                     DLQSLADLGNSYSFVQEMRFVPFGLKDVTRLAAVTAVPLLPLALTVFALEELAGYAIK
                     VLF"
     gene            complement(1479..2297)
                     /locus_tag="VIG70_18600"
     CDS             complement(1479..2297)
                     /locus_tag="VIG70_18600"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEY8252606.1"
                     /translation="MIAAGPVARAQDQGIWDRRIEEDARRETFTLTARKPNYILVTSM
                     SSPNQAPYEFTGSSDRLDSEEVKFQLAFQTKAADDLFGGNGDLWFGYTQVAFWQLFNG
                     EISAPFRETNYEPEAYLSFLTGYDLLGLKLRTVNVGVVHQSNGRAEPLSRSWNRVFAD
                     FQLTRGDFALSFKPWVRIKEDPEEDNNPDIENYLGRYELRLLYNWRGHLFSAMLRNVL
                     DSEHRLNSELAWSFPIVRRLRGLVQWYNGYGENLIDYNFNTHRIGVGVLLTDWL"
     gene            2395..3900
                     /locus_tag="VIG70_18605"
     CDS             2395..3900
                     /locus_tag="VIG70_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEY8252607.1"
                     /translation="MRLIALAFAAILGGCAGLPAGIDRPPSSVLADTGGTRLGRALAP
                     AVAVSAGKTGIHALGNARDAFAARVLLARAAERSLDVQYYIWRADTTGGLLMEALWQA
                     AERGVRVRLLLDDNNTRGMDEALATLDAHPNIEVRLFNPYANRAFRLGEMVGSFARVN
                     RRMHNKSFTADNQVAIVGGRNVGDEYFGADTPVAFDDLDVVAAGAVVSEVSRSFDAYW
                     NSDSAYPAASLIRGSPASAAQVRAAWAKLNDDPAAKKYVEALRATPLVHRLLASDLPL
                     EWARARVVSDDPAKVLHPPERTEEHMLPRLVAALGEPARQLDLVSPYFVPTKDGTAAL
                     RTLAGRGVQVRVLTNSLAATDVAPVHAGYAKYREELLRAGVRLYELKPTATQTSERGG
                     IGGSSDASLHAKTFAVDRSRIFVGSFNFDPRSARLNTEMGVVVESATLAAQLSEALDR
                     DLPRDAYEVRLVEDKLQWIDGNVRHTSEPGAGWLRRIWIGFLSILPIEWLL"
     gene            3897..4694
                     /locus_tag="VIG70_18610"
     CDS             3897..4694
                     /locus_tag="VIG70_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252608.1"
                     /translation="MKNLLILVGVACASAAHAQGEIPVHPTLTDKLYFGVGAFFPKTT
                     TSAQLTSRSGVGTTVDFEDTFGLKSGKTVPLVLARWRFGERWRLEAEYFQLNREGGRT
                     IDREIRWGENVYPVNANVQTTFDFSDLRVSVGYSFFKRPDKELGVGLGLHVAAYDVGL
                     TSNAVGSEEQDVTAPLPVLSFYGQFALTNQWALGTRVDFFSLSYENYDGRLSAVGMDI
                     TYQPFRHVGFGLGWRSLFLSLEAEKDARHLKVKQTFQGPMAFMNVSF"
     gene            complement(4699..4947)
                     /locus_tag="VIG70_18615"
     CDS             complement(4699..4947)
                     /locus_tag="VIG70_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252609.1"
                     /translation="MLFRIVLITCALLLAVSGCAGAPGGECHGVQWYRLGLNDGMADA
                     KGELERYAASCGADFGRAQYEEGFRDGLARRPKPAAKP"
     gene            complement(4974..6137)
                     /locus_tag="VIG70_18620"
     CDS             complement(4974..6137)
                     /locus_tag="VIG70_18620"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8252610.1"
                     /translation="MKLTVAVLLAAALCGCAGAPERQARKQEDLFAERRAQDAARGEG
                     AKRLVAQMLARAEAKARTPRATVDFLIISGGGDWGAFGAGVLKGWGRVKGEMARPQFD
                     AVTGVSTGALIAPFAFLGDDASIERIVQLYRNPAVDIATSRGTLFFLPNNPSFFLLPG
                     LERELRTALDRGMLERIAAQEASGRLLLVNTTNIDLGDLHAWDLVAEAKASLAAGDPQ
                     RVHRILLASAGIPAIFPAQEIGPHLYVDGAITGNILYGARVDQEQGVAAQWRAKHGRA
                     PAPRVRYWVIFNNQFRFPPQVTQARWPEIMSRATIMATQNATVSAMRHLQANAELTRL
                     KYGIDVEVRVMAVPETWVPKEAGTFKKEVMNELADVGERMGADSASWSNGVPE"
     gene            complement(6223..7341)
                     /locus_tag="VIG70_18625"
     CDS             complement(6223..7341)
                     /locus_tag="VIG70_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY8252611.1"
                     /translation="MVRLVLAIAVAFSTQALAQDRGLFTDPEDGAFDASEWLLDKKGF
                     LPVPIIVTEPAIGYGGGAALLWFRESLGERRAQGRLTPPDIFGGALAATENGTTLAGA
                     FGMLTFAQEYWRWRGFIGRPDVNLDFYGAGRDLGTGETKLGYNLEGWATTQHLLRRIG
                     DSDNWLGARWNYIDFDARFDSSHPQPLVRGAREITSSGFGVFLEHDSRDNFFTASRGW
                     KGLFEGVFYSPDLGGDASYQMYRAQAFGYVPMTKALILGGRFDSRAARGEVPFYQLPF
                     IEMRGIPFFRYQDENVALIETELRWNVTPRWSLVGFVGTGRTWGTSKSFGDADTASSW
                     GLGFRRLVARRLGIYMGVDLAKGPEETAIYIQAGSAWR"
     gene            complement(7361..7708)
                     /locus_tag="VIG70_18630"
     CDS             complement(7361..7708)
                     /locus_tag="VIG70_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252612.1"
                     /translation="MRKTAVVAVLMAACASSGNPGRYEAVLPAASGGGERHIRVVLQP
                     DGFAAVSSAFSGLPSRFLAEGTWVADGRRVTVNLDNQKTMVFERAGDQLVAREWEHSL
                     WGENGPGVLHRVR"
     gene            complement(7712..9844)
                     /locus_tag="VIG70_18635"
     CDS             complement(7712..9844)
                     /locus_tag="VIG70_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family V/XII"
                     /protein_id="HEY8252613.1"
                     /translation="MRFLFSAVLLMVACTCRAQAPAPDVAWPREAKAADGTVITVYQP
                     QVERWAEGNLSGRAAVSVKRPGEKEPHYGVIELSARTEIDKSSDVATLSSLRITKSSF
                     PGMSPEEAEKHLATLRASVTRTSWPVSAQALQANLAIAQARSQKGLPVKNDPPQILFR
                     TAPSMLVVVDGEPALRDVKDAPGLQRILNTTALIFFEKSSSTYSLRALGRWWQAPALD
                     GAWKSGPLMLATLDKPREALEKQYDPLEGKDADGKPVFEPGVTPQIIVVTKPTELLQS
                     KGEPKLSPIPRTELLYMSNSQNDIFLVLGAQTYYTLISGRWFTAKALAGPWSFVPPKS
                     LPADFAKIPADHPMADVLMSVPGTTQAREAAIANQIPQTATVQRDVQPSPVAYDGGEP
                     KWQPIDGTPLSYAPNTPAPVIRVDPKSYYMVQNGVWFSASAPPGPFVVATAVPAVIYS
                     IPASSPVHYVTYVRVYSSTPSTVFVGYTPGYYGTVMSTDGVVVYGTGYVYPPYVGPYY
                     YYPPPYYTYGYGAGFATGFFIGFAFSGGWHSPCCYGGGGGVYISHHNNININNSYNRW
                     GGKSTSVSGPGGRNVKATQVGNTTLAKGSGSNNVYAGRDGNVYRRDDSGNWQQHGGKG
                     EGWSDVGRPEQRPSAETRPSSRDAASTREATREADRSRETRQSLDRQAQSRDLGAQRA
                     QAARQPSAGFHGGGGARGGGGRGGGGRR"
     gene            complement(9841..10197)
                     /locus_tag="VIG70_18640"
     CDS             complement(9841..10197)
                     /locus_tag="VIG70_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252614.1"
                     /translation="MRSNSMSARLSLAALTCAIASCAYVEATTTQYVGVPKFPPTKPA
                     AVQVLPAEPAQRHDRLGEVLLDISVDPAPPVADIEERLREEGAKMGANAVYVIRDVIR
                     PGEGRKLIGIAIRFRQ"
     gene            complement(10187..10690)
                     /locus_tag="VIG70_18645"
     CDS             complement(10187..10690)
                     /locus_tag="VIG70_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252615.1"
                     /translation="MTRKLVIALAACALSFPAAAQQVAGVFGKGRTHVVVTAGTGYAF
                     DESYLVLGLGATYYLLDGLGVGLFFESWSGSDPGMTKITPSVQYVFHRVQVVKPYVGA
                     FYRRTDIDGLPDLDSVGARAGAYLQAGRNMYIGAGVVYESYLDCEKAVYRSCDSTYGE
                     LSFTLAF"
BASE COUNT         1768 a         3669 c         3642 g         1730 t
ORIGIN      
        1 cgaacgcacg tagcccaccg gcagcacgtg gacgcgcgtg cgggcgtcct gcagcggcag
       61 cgcgtcgaac gcaagctcgc cctgcgagta cgcatagccg gcgagcagaa aattcagccc
      121 gaccggcagg ttcgagtagg cacgcggatc cagcgactgc gccgcggcga gcgccggcgc
      181 gagcaggagc atggcgaaca cccgcatgcg tcagaaaaga accttgatcg cataacccgc
      241 cagctcttcc agcgcgaaga ccgtcagcgc cagcggcaga agaggaacgg ccgtgactgc
      301 ggcgagccgg gtcacatcct tcagtccgaa cggcacgaag cgcatttcct ggacgaagct
      361 gtagctgttt ccgaggtcgg ccagcgactg gaggtcggcg ctgccgagga gcgcctcgtc
      421 cttcggggca tcggcatgca cccacttgtc ctcgaactcc tgggtgtagc ggctcgccag
      481 cctgccgaac tcgcccatgc cttgccgctt cgcgcgcacc aggtgcggcg tgaatacggt
      541 cagcgggatg aagacgacga cgacgaagaa agcgatgaag gctccgattt ccaccttgaa
      601 gtccgtcagc tgctgtcccg catagaagat ctggctggcg agcaatccgg ccagcacggc
      661 gccctgggca aaaagaatcg gcgcgaaggc gttggtgctc ctgccgagaa agcttagccc
      721 ggcggtcttg tccgggtgga tgggaaccag gcgcaggttc agccgcgaga cccgccagag
      781 aaacgagaac cagaggaaaa aacgcagata ccagcgcagc aggatgaact ggaaaatcgg
      841 caagctgatg aagaaatacc agtatccggc cggcgtggga tgcatccctt cgccatccgg
      901 ggtcgcgtac cagcttgccg tctcgagcgc gatctggttg cgccagacaa acaggccaag
      961 cgtgtagacc acgacgatga gcgtcacctc cccaatcacg gagttacgca tgcgcaaggt
     1021 gctgtcgatg gcctcctgaa acttcggcag ctcttccggc acgacgatgc gccgctccac
     1081 gaactgagtg acgaccgggc ggatgcgaag gtggacgacc agctcggcgg cgatcaggat
     1141 gggcagcgcg atcaagaagc gggcgtgcgc ctctatgtcg tagaggaatg gaattcccgc
     1201 tgcctcgtca tgcgttctgc ccgaaagcgc cgccagcagc agcaacggca gccacgcgac
     1261 gccggtgatg accagtatcc gccgccacag catctccagc gcgtccccgg tcaagtgcga
     1321 tcggcggaac aactggtaga gcggtccccc gagcaccagg gagaagtcgg gtagttgcag
     1381 gatggcggac ttggtcatgg ggtctccctt aatcgcaaat catctcggtg ggcgtgacgg
     1441 cgcttcatcc gcgccgccat tggacctggg tcccatggct acaaccagtc ggtcagcagc
     1501 actccgacgc cgatgcggtg ggtgttgaag ttgtagtcga tcaggttctc gccatagccg
     1561 ttgtaccact gcaccaggcc gcgcagccgg cggacgatcg ggaacgacca ggcgagctcg
     1621 gagttcagcc ggtgctcgct gtcgagcacg ttgcgcagca tggcgctgaa gaggtgccct
     1681 ctccagttgt agagaaggcg cagctcatag cggccgaggt agttctcgat gtccgggttg
     1741 ttgtcttcct ccgggtcctc cttgatgcgc acccagggct tgaagctcag cgcgaagtcg
     1801 ccgcgcgtga gctggaagtc ggcgaacacg cggttccagc tgcgcgagag cggctccgcc
     1861 cggccattcg actggtgcac cacgccgacg ttgaccgtgc gcaacttcag gccaaggagg
     1921 tcgtagcctg tcaggaacga aagataggct tcgggctcgt agttggtctc gcggaacggc
     1981 gccgagatct cgccgttgaa gagttgccag aacgccacct gcgtgtagcc gaaccacagg
     2041 tcgccgttgc cgccgaagag gtcgtcggcc gccttggtct ggaaggcgag ctggaacttc
     2101 acttcctcgc tgtcgagccg gtcggaggag ccggtgaact cgtagggcgc ctggttgggc
     2161 gagctcatcg aggtgaccaa gatgtagttc ggcttgcgcg ccgtgagcgt aaaggtttcg
     2221 cgccgcgcgt cctcctcgat gcgccggtcc cagatgccct ggtcctgcgc gcgcgcgacc
     2281 ggacccgcgg cgatcaatgc gcccgcagca agcgctatcc actccggagc tcggctcatt
     2341 gcacgccaac gataacaggg ctcatcatgt ccgctattgg accttggtcc cacaatgcgc
     2401 ctgatcgcct tggcgttcgc ggcgatcctg ggaggatgcg ccggcttgcc ggccggcatc
     2461 gaccgtcctc cctcgagcgt gctcgctgat accggcggca cccggctcgg ccgcgccctc
     2521 gcgcctgcgg ttgcggtcag cgccgggaaa accggaatcc acgcgctcgg caacgcgcgc
     2581 gatgcgttcg ccgcgcgcgt gctgctggcg cgcgccgccg agcgcagcct cgacgtgcag
     2641 tactacatct ggcgcgccga cacgaccggc ggactgctga tggaagcgct ctggcaggcg
     2701 gccgagcgcg gcgtgcgcgt gcggctgctg ctcgacgaca acaacacccg cggcatggac
     2761 gaggcgctcg cgacgctcga cgcgcatccc aacatcgagg tgcgcctctt caacccgtac
     2821 gccaaccgcg ccttccgcct cggcgagatg gtcggcagct tcgcgcgcgt caaccgccgc
     2881 atgcacaaca aatcgtttac cgcggacaac caggtggcga tcgtcggcgg ccgcaacgtc
     2941 ggcgacgagt acttcggcgc ggacacgccg gtggcattcg acgacctgga cgtcgtcgct
     3001 gcaggcgcgg tggtgagcga agtctcgagg tcgttcgacg cgtactggaa cagcgactcc
     3061 gcctacccgg cggcaagcct gatccgcggg tcacccgcaa gcgcagcgca ggtccgcgca
     3121 gcgtgggcaa agctcaacga cgaccccgcg gcgaagaaat acgtcgaggc gctgcgcgct
     3181 acgccgctgg tgcatcggct attggcaagc gacctgccgc tcgaatgggc gagggcccgc
     3241 gtcgtcagcg acgatccggc caaggtactg catcccccgg agcgcaccga ggagcacatg
     3301 ctgccgcgcc tcgtcgcggc gctcggcgag cccgcgcggc agctcgacct ggtgtcgccg
     3361 tacttcgtgc cgacgaagga cggcacggcg gcgctgcgca cgctggccgg gcgcggcgtc
     3421 caggtgcgcg tcctgaccaa ctcgctcgct gcgaccgacg tcgcgccggt gcacgccggc
     3481 tacgcgaagt accgcgaaga gctgctgcgc gccggcgtgc ggctctacga gctgaagccg
     3541 accgcgacgc aaacgagcga gcgcggcggc atcggcggca gctcggacgc aagcctgcac
     3601 gccaagacgt tcgcggttga ccgcagccgc atcttcgtcg gctcgttcaa cttcgacccg
     3661 cgctcggcgc ggctcaacac cgagatgggc gttgtggtcg aaagcgctac gctcgcggcg
     3721 cagctatccg aggcgctcga ccgcgatctg ccccgcgatg cctacgaagt gcggcttgtc
     3781 gaagacaaac tccagtggat tgacggcaat gtgcgccaca cgtcggagcc gggcgcggga
     3841 tggctgcgcc gaatttggat cggctttctt tcgattctcc ccatagaatg gctcctatga
     3901 aaaatctgct gatccttgtc ggcgtcgcat gcgcgagcgc agcgcacgcg cagggcgaaa
     3961 ttcccgtcca ccccacgctg accgacaagc tctacttcgg cgtcggcgcc ttcttcccca
     4021 agaccacgac ctcggcccag ctcacctcgc gcagcggtgt cggcacgacg gtcgacttcg
     4081 aggacacgtt cggcctcaag tcggggaaga ccgtcccgct cgtgctcgcg cgctggaggt
     4141 tcggcgagcg ctggcgcctc gaggcggaat attttcagct caatcgcgag ggcgggcgga
     4201 cgatcgaccg cgaaatccgg tggggagaga acgtctaccc ggtgaacgcc aatgtgcaaa
     4261 cgacgttcga cttctccgac ctgcgcgtct ccgtcgggta ctcgttcttc aagcggcccg
     4321 acaaggagct cggggtcggg ctgggattgc acgtcgccgc ttacgacgta ggccttacct
     4381 ccaacgcggt cggcagtgag gagcaggacg tgaccgcccc gctgccggtg ctcagcttct
     4441 acggccagtt cgccctgacc aatcagtggg cgctgggcac ccgcgtcgac ttcttctccc
     4501 tctcctacga gaactacgac ggcaggctca gcgcggtcgg catggacata acgtaccagc
     4561 cgttccgcca cgtgggcttc ggcctgggct ggcgcagcct gttcctcagc ctcgaggcgg
     4621 agaaggacgc gcggcacctg aaggtcaagc agaccttcca ggggccgatg gcgttcatga
     4681 acgtgagctt ctgagcgtct acggtttcgc ggccggcttc ggccggcgcg caagcccgtc
     4741 ccgaaagcct tcctcgtatt gcgcgcggcc gaaatccgct ccgcagctcg ccgcgtagcg
     4801 ctcgagctcc cccttcgcgt ccgccatgcc gtcgttcagg ccgagccgat accactggac
     4861 gccatggcat tcgccgccgg gcgcgccggc gcagcccgac accgcgagga gcagcgcgca
     4921 ggtgatcaag acgatgcgaa aaagcatggc gtctcctttt cttcgacctt gcgttattct
     4981 ggcaccccgt tgctccagct cgccgaatcg gcgcccatcc tctcgccgac gtcggccagc
     5041 tcgttcatca cttccttctt gaaggtgcct gcctctttcg gcacccaggt ttcgggtacc
     5101 gccatgacac gcacctccac gtcgatgccg tacttgaggc gggtcagctc ggcgttcgcc
     5161 tggaggtggc gcatggcgct caccgtcgcg ttctgcgtcg ccatgatggt ggcgcgcgac
     5221 atgatctcgg gccagcgcgc ctgcgtcacc tgcggcggga agcggaactg gttgttgaag
     5281 atcacccagt agcgcacgcg cggcgccggc gcgcggccgt gcttcgcgcg ccactgcgcg
     5341 gcgaccccct gctcctggtc gacgcgcgcg ccgtacagga tgttgccggt gatcgcgccg
     5401 tcgacataca agtgcgggcc gatctcctgc gccgggaaga tcgccgggat gccggcgctc
     5461 gccagcagga tgcggtggac gcgctgcggg tcgcccgcgg cgagcgacgc cttcgcttcc
     5521 gccaccaggt cccaggcgtg caggtcgccg aggtcgatat tggtggtgtt gaccagcagc
     5581 aggcgtccgc tcgcctcctg cgcggcgatg cgctcgagca tgccgcggtc gagcgcggtg
     5641 cgcagctcgc gctcgaggcc ggggagaaga aagaacgacg gattgttcgg cagaaagaac
     5701 agcgtcccgc gcgacgtcgc gatgtcgacc gccgggttgc ggtacagctg cacgatgcgc
     5761 tcgatcgacg catcgtcgcc gaggaaggcg aacggcgcga tcagcgcgcc ggtgctcacg
     5821 ccggtgaccg cgtcgaactg cggccgcgcc atctcgccct ttacgcgccc ccagcccttc
     5881 agcacgccgg cgccgaacgc gccccagtcg ccgccgccgg agatgatcag gaagtcgacg
     5941 gtcgcgcgcg gcgtacgcgc tttcgcctcc gcgcgcgcaa gcatctgcgc gaccaggcgc
     6001 ttcgcgccct cgccgcgagc ggcatcctgc gcccggcgct cggcgaacag gtcctcctgc
     6061 ttgcgcgcct gccgctcggg cgcgccggcg cagccgcaca gcgcggcggc gaggaggacg
     6121 gcaacggtca gcttcatggg tcgttcacgt ttcccgaggc tcatcccacc gcagtctccg
     6181 ccggcggcgc ccgggctcct attggaccat ggtcccatgc ccctaccgcc aggcgctgcc
     6241 cgcctgaatg tagatcgccg tttcctcggg ccccttggcg aggtcgacgc ccatgtagat
     6301 tccgagccgc cgcgcgacca ggcgacggaa gccgagcccc catgagcttg ccgtatcggc
     6361 gtcgccgaag ctcttgctgg ttccccaggt gcggccggtg ccgacgaagc cgaccagcga
     6421 ccagcgcggc gtgacgttcc agcgcaactc ggtctcgatc agcgcgacgt tctcgtcctg
     6481 gtagcggaag aacggtatgc cgcgcatctc gatgaacggc agctggtaga acggcacctc
     6541 gccgcgcgcg gcgcgcgagt cgaagcggcc gccgaggatg agcgccttcg tcatggggac
     6601 gtagccaaaa gcctgggcgc ggtacatctg gtagctcgcg tcgccgccga ggtcgggcga
     6661 atagaacacc ccctcgaaga ggcccttcca gccgcgcgag gcggtaaaga aattgtcgcg
     6721 cgagtcgtgc tccaggaaca cgccgaagcc cgagctggtg atctcgcgcg cgccgcgcac
     6781 cagcggctgc ggatgcgacg aatcgaagcg cgcgtcgaaa tcgatgtagt tccagcgcgc
     6841 gccgagccag ttgtcgctgt cgccgatccg ccgcagcagg tgctgcgtcg tcgcccagcc
     6901 ctcgaggttg tagcccagct tggtttcgcc ggtgccgagg tcgcgcccgg cgccgtagaa
     6961 atcgaggttg acgtccggcc gcccgatgaa tccgcgccag cgccagtact cctgggcgaa
     7021 ggtcagcatg ccgaaggcgc cggcaagcgt ggtgccgttc tcggtcgcgg cgagcgcgcc
     7081 gccgaagatg tcgggcgggg tgaggcggcc ttgcgcgcgc cgctcgccga gcgactcgcg
     7141 gaaccagagc agcgccgcgc cgccgccgta gccaatcgcc ggctcggtga cgatgatcgg
     7201 caccggcagg aagcccttct tgtcgagcag ccactcggag gcgtcgaacg cgccgtcctc
     7261 gggatccgtg aacaggccgc ggtcctgtgc gagcgcctgg gtcgagaaag caactgcaat
     7321 tgcaagaacc agcctcacca cgaatggccg accccgcctc tcaccggacc cggtgcagca
     7381 cgccgggacc gttctcgccc cacaggctgt gctcccactc gcgggcgacg agctggtcgc
     7441 cggcgcgctc gaacaccatg gttttttgat tgtcgagatt gaccgtgaca cggcgaccgt
     7501 ccgctaccca ggttccctcg gcaaggaacc ggctcggaag gcccgagaag gcgctggaca
     7561 cggcggcgaa cccgtcgggc tggagcacga cgcggatgtg gcgctcgccg ccgccgctcg
     7621 cggccggcag caccgcctcg taccggccgg ggtttccgct cgaggcgcaa gccgccatca
     7681 gcacggccac gacggcggtc ttgcgcattt cctagcggcg tcctccgccg ccgcggccgc
     7741 cgccgcctcg cgcgccgccg ccgccgtgga agcctgcgct cggctgccgg gccgcctgcg
     7801 cgcgctgcgc gccgaggtcg cgcgactggg cctgccggtc gagggactgc cgggtttcgc
     7861 ggcttcggtc tgcttcacgc gtcgcctctc gtgtcgaagc agcgtcgcgc gacgacgggc
     7921 gcgtttcagc cgagggcctt tgctccggtc tgccgacgtc cgaccacccc tcacccttgc
     7981 cgccgtgctg ctgccagttg ccgctgtcat cgcgccggta cacgttgccg tcgcggccgg
     8041 cgtacacgtt gttgctgccc gagcccttgg cgagggtcgt attcccgacc tgcgtcgcct
     8101 tcacgttgcg cccgccgggg ccgctgacgc tggtgctctt gccgccccag cggttgtagc
     8161 tgttgttgat gttgatattg ttgtggtggc tgatgtacac gccaccaccg ccgccatagc
     8221 agcaggggct gtgccagccg ccgctgaacg cgaagccgat gaagaagccg gtggcgaagc
     8281 ccgcgccgta gccgtaggtg tagtacggcg gcggatagta gtagtacggc ccgacgtagg
     8341 gcgggtagac gtagcccgtg ccgtagacca ccacgccgtc ggtggacatc actgtgccgt
     8401 agtagcccgg cgtgtagccc acgaacaccg tgctcggcgt gctgctatac acgcgcacgt
     8461 aggtcacgta gtgcaccggc gagctcgccg ggatgctgta gatcaccgcg ggaaccgcgg
     8521 tcgccacgac aaagggcccc ggcggcgcgc tggccgaaaa ccagacgccg ttctgcacca
     8581 tgtagtagga cttcgggtcg acgcggatca ccggcgcggg ggtgttggga gcgtagctga
     8641 gcggcgtgcc gtctatgggc tgccacttcg gctcgccgcc gtcataggcg accggcgagg
     8701 gctgcacgtc gcgctgcacg gtcgcggtct gcgggatctg gttggcgatc gccgcctcgc
     8761 gcgcctgcgt cgtgcccggc accgacatca gcacgtcggc catcgggtgg tccgccggga
     8821 tcttcgcgaa atccgcgggc aggctcttgg gcgggacgaa gctccacggc ccggcgagcg
     8881 ccttcgcggt gaaccagcgc cccgagatca gcgtgtaata ggtctgcgcg ccgagcacca
     8941 ggaagatgtc gttctgcgag ttcgacatgt agaggagctc ggtgcgggga atcggcgaga
     9001 gcttcggctc gcctttcgat tgcagcagct cggtgggctt cgtcaccacg atgatctgcg
     9061 gcgttacgcc cggctcgaac accggcttgc cgtcggcgtc cttgccctcg agcgggtcgt
     9121 actgcttttc gagcgcttcg cgcggcttgt cgagcgtcgc cagcatcagc ggcccggact
     9181 tccaggcgcc gtccagcgcc ggcgcctgcc accagcggcc gagcgcgcgc agggaatagg
     9241 tggaggaaga cttctcgaaa aagatcagcg cagtggtatt gaggatgcgc tgcagcccgg
     9301 gcgcatcctt cacgtcgcgc agcgccggct cgccgtcgac caccaccagc atggacgggg
     9361 cggtgcggaa caggatctgc ggcgggtcgt tcttcaccgg cagccccttc tgcgagcgcg
     9421 cctgcgcgat cgcgagattg gcctgcagcg cctgcgccga taccggccag ctcgtccttg
     9481 tcaccgaagc gcgcagcgtg gcgagatgct tctcggcctc ttccgggctc atgccgggaa
     9541 agctcgactt ggtgatgcgc agcgacgaga gcgtcgccac gtcggaagac ttgtcgatct
     9601 cggtgcgcgc ggagagctcg atcacgccgt agtgcggctc cttctcgccc ggccgcttga
     9661 ccgacactgc cgccctgccc gacaggttgc cctccgccca gcgctcgacc tgcggctggt
     9721 acacggtgat caccgtgccg tcggcggcct tcgcctcgcg cggccatgcg acgtcgggag
     9781 cgggagcctg cgcccggcag gtacaggcga ccatcaggag aactgcggag aagagaaatc
     9841 tcattggcgg aaccggatcg cgatgccgat gagcttgcgc ccctctcccg gcctgattac
     9901 atcgcggatg acgtagacgg cgttcgctcc cattttcgct ccctcttcgc gcagccgctc
     9961 ttcgatatcg gcgaccggcg gcgccgggtc gacggagatg tcgagcagga cctcgccgag
    10021 gcgatcgtgc cgctgcgccg gctcggccgg cagaacctgc accgcggcgg gcttggtcgg
    10081 cggaaacttt ggcacgccga catactgcgt cgtagtggct tcgacgtagg cgcacgacgc
    10141 gatcgcgcac gtcagcgcgg cgagtgataa ccgggcgctc atcgaattag aacgcaagcg
    10201 tgaagcttag ctcgccgtaa gtgctgtcgc acgagcggta gacggccttt tcgcaatcga
    10261 ggtaggactc gtagaccacg cctgcgccga tgtacatgtt gcgcccggcc tggaggtagg
    10321 cgccggcgcg cgcgccgacg gaatcgaggt ccggcaggcc gtcgatatcg gtgcggcgat
    10381 agaacgcgcc gacatagggc ttcacgacct gcacgcggtg gaacacgtac tgcaccgaag
    10441 gcgtgatctt cgtcatgccg gggtccgagc cggaccagga ctcgaagaaa aggccgacgc
    10501 cgagcccgtc gagcaggtag taggtcgcgc cgagcccgag gacgaggtag ctttcgtcga
    10561 acgcgtagcc ggtgccggcg gtcacgacga cgtgcgtgcg gcccttgccg aagacccccg
    10621 cgacctgctg cgccgctgcc gggaagctca atgcgcatgc cgcgagcgca ataaccaatt
    10681 tccgggtcat cagaacttgg tgccgaagtc gatgcggtac tgcgtccagg cctgcgagcc
    10741 gtagccgtcg ctgaattgga tccactgctg gttcaccagc ccgcggaaga agcccctgcc
    10801 gacgtccag
//
LOCUS       DATBSZ010000286         7629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1492857, whole genome shotgun sequence.
ACCESSION   DATBSZ010000286 DATBSZ010000000
VERSION     DATBSZ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492857"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /gene="mobA"
                     /locus_tag="VIG70_18650"
     CDS             <1..417
                     /gene="mobA"
                     /locus_tag="VIG70_18650"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEY8252616.1"
                     /translation="NANQNIERYESYGHRVVPDEIGGFAGPLAGLHAGLKAAREPHVV
                     TVPCDSPFLPSDLVLRLSNSLGENHLAVARTGDQPHPVFALMKRQVRESLEAFLATGG
                     RKIDAWYAALKVVEVSFDDEADAFRNINTLEELKRE"
     gene            414..1658
                     /gene="glp"
                     /locus_tag="VIG70_18655"
     CDS             414..1658
                     /gene="glp"
                     /locus_tag="VIG70_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY8252617.1"
                     /translation="MSIAQTVSCLDGYDPDALRVDKAREAILACLTPINDSENLKIRE
                     ALGRVLSQDIVPHIDVPAHDNSAMDGYAVRFSDLEKQPLKEIGTALAGKPFHGKLGAG
                     ECVRIMTGAVMPAGADTVVIQEVVKKEGERVTIPAGQKKAQNVRYAGEDLKVGTPVLR
                     SGKLLTPADIGLVASLGMGEVRVKRRLRVAFFATGDELASIGAPLKEGEVYDSNRYTL
                     HGMLARLGVEIVDMGVVRDDPAALESAFLLASRQDVVITTGGVSVGEADFVKQLMAKL
                     GEVLFWKIAMRPGRPMAFGKIGNAFLFGLPGNPVAVMVTFYQFVRDALIHLSGRNDDA
                     LPLLKAVAAENLRKVPGRTEYQRGILFRDQDQWKVRTTGQQGSGVLRSMSEANCFIVI
                     EHERAAVKAGELVSVQLFEGLA"
     gene            1655..2143
                     /locus_tag="VIG70_18660"
     CDS             1655..2143
                     /locus_tag="VIG70_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEY8252618.1"
                     /translation="MNPIHPALAELARAAKEMPGRDVAPVLKEALSENRWLRPEHRIG
                     DPAGYMRHVLYVDPEDAFVITAITWLPGQVSPVHGHRVWCAYGIAEGELTEEQFRESG
                     GNLSPLKNAVYRAGDIAERDLECRTIHRVSNRGRGTLVSLHVYGVSAERLTTGINRLF
                     PA"
     gene            2220..2432
                     /locus_tag="VIG70_18665"
     CDS             2220..2432
                     /locus_tag="VIG70_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252619.1"
                     /translation="MVHFMDAFRRNADGSWTCIAPVTLSNPSGPVRFTEGATFPQGMM
                     FMGIKVATLLDELAKYPRPGHRQTSL"
     gene            complement(2437..2769)
                     /locus_tag="VIG70_18670"
     CDS             complement(2437..2769)
                     /locus_tag="VIG70_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252620.1"
                     /translation="MSEPTRKSQIDAVLLKLPGVSTRKINGLDAYFVSDRMFACISGD
                     GVGLRLPVAAATEAQFSRDDVVPFQPRGLASSREWIQINRADAADYEKDLELFRASLE
                     FVSGGRTR"
     gene            2786..3481
                     /locus_tag="VIG70_18675"
     CDS             2786..3481
                     /locus_tag="VIG70_18675"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8252621.1"
                     /translation="MAGRSSLAVSSSLALKGVLEKSAPAFEQSLGVRLDMRFDATQAI
                     LAEFEAGLRADLLVLTAEAMEALRASGKVAQVRALGSSGVGIAVRAGAPKPDISSVDA
                     LKRALVSARSVAHSKVGASGIYFAELLQRFDIRLKKRVVVEKGPVGLVVAAGEAELGV
                     QQLCELAPVPGIEIVGPLPEPVQRLTHFAAGIPANAANPKGASALLALLRTDAVRADM
                     VRGGIVPSSLAEA"
     gene            complement(3464..5665)
                     /locus_tag="VIG70_18680"
     CDS             complement(3464..5665)
                     /locus_tag="VIG70_18680"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEY8252622.1"
                     /translation="MRKILAVGAGTALLVALYAAAGTWIAPRYVREALVDAAAKQGFN
                     LELKTVRTHPFALSVALEGIELRGPLGRNAAARTASVDLSSASLWHRAWVVRELAVEG
                     GTVQVAGQGLALQELTFHARDLSTGEAQPGRFEAAGRLAGGAGRIAARGALSLAPLAL
                     HEVVIEGSGLAYAGVELKQATLEAPMVPVPPREPIALALRASVAPQGTIVAHGTVGLQ
                     PQRADLEIEFDALPLAPAQRWLPDAALKIASGALSGKGALQIGEATAAYEGSAAVHAL
                     RLTERDSGAPLLAWQHAGTDSLKLALAPVSVEIRELVLRAPEGRLVIEQDGSMNFAKV
                     FGGGDATEKDTPLRASVERLRIESGTLHFADRSLANPFEVTIRDLAGGITGVSTAAGK
                     EPARVRLNGRVQPYGVARIRGTIDLNAPTSLADVRASLRNLHLEAFNPYIAKFAGYRI
                     ESGRVSADLRYELRAGRLVGTNKLAFESMKLGEKLETEGLFDLPLELAVALLADAEGR
                     IDLDIPVRGNLNDPQFDLGTIMARAFGNVLKKIVSAPFRALAGLFGGEDRDPGAVAFA
                     PGSAALTPPAEEDVAQVAKALEERPRLGIRVHGGYDPARDPDALRLRAARDELAHAAG
                     LKGSLDLSDPKVVRAAERLYLKHGGERAGLADLRKGEPRYVRALLQRLAAAMPADPAA
                     AETLARERAEAVRAALLDHGVEPERVQVAATVQQQAEGEGVPTRLALGLGQ"
     gene            complement(5694..6713)
                     /locus_tag="VIG70_18685"
     CDS             complement(5694..6713)
                     /locus_tag="VIG70_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8252623.1"
                     /translation="MKLAHIAALLFCFTSFQAGAQSPLTVYCSIIDEQCRAGVAAFER
                     ATGTKVTMVRKGTGETYAQIRAEASSPRGDVWWGGPGDPHMQAAEEGLLEEYKSPKLA
                     ELHPWAVRLAELSKFRTTGIYLGALGIGYNTDVLKKKNIPEPKCWADLLDARFRDEVQ
                     IADPNSSGTAYVMLATIVQLMGEDKGFAYLKALHRNVNQYTKSGIAPVKSTASGETGV
                     GIAFLHDMVTMRVQGAPIQTVAPCEGTGYEIGAVSLIKGRKNAEAAKRFLDWTLSAQA
                     QKIVGVDQNIYSIPSNKAAPVHAQAPKIAAIKLIDYDSAKYGSSAERNRLLKKWGDEV
                     KAAPK"
     gene            complement(6732..>7629)
                     /locus_tag="VIG70_18690"
     CDS             complement(6732..>7629)
                     /locus_tag="VIG70_18690"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEY8252624.1"
                     /translation="GALARGWEVFGVRNGYAGLLGDLIEPLAARDVGGIVQSGGTVLG
                     SARCPEFVEAAGRAKALSNLKKKSIDALVVIGGNGSQSGSASLAREGFAVVGVPSTID
                     NDLYGNDVSIGCDTAINITLEAIDRLRTTASSHQRAFAVETMGRDCGYIALMAGIAGG
                     AEVIALPESEILPSQVAERLRDAYRRGKTHALAVIAEGAKCGVHELMEYYAARRADIG
                     FELRVTRLGHVVRGGIPGAADRVLATRLGAAAIETLAAGRHGVLVGIVKNDIVTTPLA
                     DITGRTRPADASLLELARVMAI"
BASE COUNT         1189 a         2599 c         2636 g         1205 t
ORIGIN      
        1 aacgccaatc agaatatcga gaggtatgaa agctacgggc accgcgtggt gcccgacgag
       61 atcggcggct tcgccggccc gctcgccggc ctgcacgcgg gcctcaaggc cgcgcgcgaa
      121 ccgcatgtcg tcacggtgcc ctgcgattcg ccgttcctgc cgtccgacct cgttttgcgt
      181 ttgagcaatt ctctgggcga aaaccatctc gccgtcgcca ggaccggcga tcagccgcac
      241 ccggtgttcg cgctcatgaa aaggcaggtg cgcgagagcc tggaggcgtt tctcgcgacg
      301 ggcggccgca agatcgacgc ctggtatgcc gcgctcaagg tggtcgaggt gagcttcgac
      361 gacgaagccg acgccttccg caacatcaac accctcgagg agctgaagcg ggaatgagca
      421 ttgcgcagac ggtgagctgc ctcgacggct acgatcccga cgcgctgcgg gtcgacaagg
      481 cgcgcgaggc catactcgcc tgcctcacac cgataaacga ctctgaaaac ctgaaaattc
      541 gcgaagcgct gggccgcgtt ctatcgcaag acatcgttcc gcacatcgac gtcccggcgc
      601 acgacaactc ggcgatggac ggttatgcgg tgcgcttttc cgatctcgaa aaacagccgt
      661 tgaaggaaat cggaacggcc ctggccggca agcctttcca cggcaagctc ggcgcgggcg
      721 agtgcgtgcg catcatgacc ggcgcggtga tgcctgccgg cgccgacacc gtggtcatcc
      781 aggaggtggt gaagaaggaa ggcgagcgcg tcactatccc ggcgggacag aagaaggcgc
      841 agaacgtgcg ctacgccggc gaagacctga aggtcggcac cccggttttg agaagcggaa
      901 agctcctgac gccggccgac atcggcctcg tcgcctcgct cggcatgggc gaggtccggg
      961 tgaagcgcag gctgcgcgtg gcgttcttcg ccaccggcga cgagctcgcg tcgatcggcg
     1021 cgccgctcaa ggaaggcgag gtctacgact cgaaccgcta cacgctgcac ggcatgctgg
     1081 cgcggctcgg ggtcgagatc gtcgacatgg gcgtagtgcg cgacgatccg gcggcgttgg
     1141 agagtgcttt tctccttgct tcaaggcagg acgtggtcat caccacgggc ggcgtctcgg
     1201 tgggcgaggc cgacttcgtc aagcagctga tggcaaagct cggcgaggtg ctgttctgga
     1261 agatcgcgat gcgccccggg cggccgatgg cgttcggcaa gatcggcaac gcgttcctct
     1321 tcgggctgcc gggaaatccg gtcgcggtga tggtcacctt ctaccagttc gtgcgcgacg
     1381 cgctcatcca tctctcggga aggaacgacg atgcgctgcc gctgctgaag gcggtggctg
     1441 ccgagaacct gcggaaagtc cccggccgca ccgaatacca gcgcgggatc ctgttccggg
     1501 accaggatca gtggaaggtg cgaaccaccg gccagcaggg ctccggcgtg ctgcgctcga
     1561 tgtcggaagc gaactgcttc atcgtgatcg agcacgagcg cgcggcggtc aaagccggcg
     1621 agctcgtgag cgtgcagctc ttcgaaggcc tggcgtgaac ccgatccacc cggcgctcgc
     1681 ggagctcgcg cgggcggcga aggaaatgcc gggcagagac gtggcgccgg ttctcaagga
     1741 agccctgagc gagaaccgct ggctgcggcc ggagcatcgc atcggcgacc cggccggcta
     1801 catgcgccac gtgctttacg tcgaccccga agacgccttt gtcatcacgg cgatcacctg
     1861 gctgccgggc caggtgagcc cggtgcacgg ccaccgcgtc tggtgcgcct acggcatcgc
     1921 cgagggcgag ctgaccgagg agcagtttcg cgagtccggc gggaacttga gccccctgaa
     1981 aaacgcggtc taccgggcgg gcgacatcgc cgagcgcgat ctcgagtgca ggacgatcca
     2041 ccgcgtgtcc aatcgcggcc gcggcaccct cgtctcgctg cacgtctacg gcgtgagcgc
     2101 cgagcggctg acgaccggga tcaaccggct atttcccgcc taaacagggc atttcggcct
     2161 aatcgtcccc ttgagcgggg ctgtcgcagg gcggtaaagt acgcccgagg gggaggatca
     2221 tggtgcactt catggacgcc ttccgcagaa atgcggacgg gtcctggacc tgcattgcgc
     2281 cggtcacgct gagcaatccc agcggcccgg tgcgctttac ggaaggggcc acctttccgc
     2341 aaggcatgat gttcatgggc atcaaggtcg ccaccctgct cgacgagctg gcgaagtacc
     2401 cgcgccccgg gcacaggcag acaagcctgt aaaggtctag cgggtccggc cgccgctcac
     2461 gaattccagc gaggcgcgaa acagctccag gtccttctcg tagtcggccg cgtcggcgcg
     2521 gttgatctgg atccactccc gggagctcgc aagcccgcgc ggctgaaacg gcaccacgtc
     2581 gtccctcgag aactgcgcct cggtcgcggc ggcgacggga aggcgcagtc cgaccccgtc
     2641 gccgctgatg cacgcgaaca tccggtcgct cacgaaatag gcgtcgaggc cattgatctt
     2701 gcgcgtgctg accccgggga gcttgaggag cacggcatcg atctgcgact tgcgggttgg
     2761 ttcggacaag cgattaaatt gtcgcatggc cggacggtcg agcctcgcgg tgtcgagctc
     2821 cctcgcgtta aaaggcgtgc tggagaaatc cgcgccggca ttcgagcaat ccctcggcgt
     2881 gcggctcgac atgcgcttcg atgcaacgca ggcaatcctg gccgagttcg aggcgggcct
     2941 gcgggcggat ctgctggtcc tgaccgccga ggcgatggaa gcgttgcgcg cgagcggcaa
     3001 ggtggcgcag gttcgcgccc tcggcagctc gggcgtcggc attgcggtcc gcgccggcgc
     3061 gccgaagccg gatatcagct cggtcgacgc gctcaagcgt gcgctcgtct ccgcgcgctc
     3121 ggtcgcgcac tcgaaggtcg gcgcgagcgg aatctatttc gccgagctgc tccagcgctt
     3181 tgacataaga ctgaagaagc gagtggtcgt cgaaaaggga ccggtcggcc tggtggtggc
     3241 ggcgggggaa gccgagctcg gcgtgcagca actttgcgag ctggcgccgg tccccgggat
     3301 cgagatcgtc ggcccgctgc cggagccggt ccagagactg acgcatttcg cggccgggat
     3361 tccggcgaat gccgcgaatc cgaagggcgc ttcggcgctg ctcgcattgc tgcgcaccga
     3421 tgccgttcgc gccgacatgg tcagaggcgg cattgttccg tcctcactgg ccgaggccta
     3481 gcgcaagccg ggtcggcacg ccctcccctt cggcctgttg ctgcaccgtc gctgcgacct
     3541 gcacgcgctc cggctcgacg ccgtggtcga gcagcgccgc ccggacggcc tccgcgcgct
     3601 cccgggcgag cgtctccgcc gccgcgggat cggcgggcat ggcggcggcg agcctttgca
     3661 gcagcgcccg gacgtaacgc ggctcgccct tgcgcaggtc ggccagccca gcgcgttcgc
     3721 cgccgtgctt cagatacagc cgctcggccg cgcgcacgac cttcggatcg gacaggtcga
     3781 gcgaaccttt gagccccgcg gcgtgggcaa gctcgtcgcg cgccgcgcgc aggcgcagcg
     3841 cgtccggatc gcgcgcgggg tcgtagccgc cgtgcacccg gatgccgagc cgcgggcgct
     3901 cctcgagcgc cttcgccacc tgcgcgacgt cctcctccgc agggggcgtg agcgcggcgg
     3961 agccgggcgc gaaggccacc gcgcccggat cgcggtcctc gccgccaaag agcccggcga
     4021 gcgcgcggaa cggcgccgac acgattttct tcagcacgtt gccgaaggcg cgcgccatga
     4081 tggtgccgag gtcgaactgc ggatcgttca ggttgccgcg caccgggatg tcgaggtcga
     4141 tgcggccttc cgcgtcggcg agcagcgcca ccgcgagctc gagcggcagg tcgaatagcc
     4201 cctctgtttc gagcttttcg ccgagcttca tgctctcgaa cgcgagcttg ttggtgccca
     4261 ccaggcggcc ggcgcgcagt tcgtagcgca gatcggccga cacgcggcca gactcgatgc
     4321 gatagccggc gaacttcgcg atgtacgggt tgaaggcctc gaggtgcagg ttgcggaggc
     4381 tcgcccggac gtcggctagg ctcgtcggcg cgttcaggtc gatcgtcccg cggatgcgcg
     4441 ccactccgta gggctgcacg cggccgttga ggcgcacgcg tgcaggctcc ttccccgccg
     4501 cggtgctgac gccggtgatc ccgccggcca ggtcgcggat cgtgacttcg aacggattgg
     4561 cgagcgagcg atcggcgaag tgcagcgtgc cgctctcgat gcgcaggcgc tcgacgctgg
     4621 cgcgcaacgg cgtgtccttt tcagttgcat caccaccgcc gaaaaccttc gcgaaattca
     4681 tcgagccgtc ctgctcgatt accaaacgcc cttccggcgc acgcagcacc agctcacgga
     4741 tctcgacgct gaccggtgcc agcgcgagct tcagcgagtc ggtcccggca tgctgccagg
     4801 cgaggagcgg cgcgcccgaa tcgcgctccg tcaggcgcag tgcatgcacg gcagccgagc
     4861 cctcgtacgc tgcggtcgcc tcgccgatct gtagcgcccc tttgccggac aacgcgcccg
     4921 aggcaatctt cagggcggcg tccggcagcc agcgctgcgc cggcgcgagc ggcagcgcat
     4981 cgaactcgat ctcgaggtcg gcgcgctgcg gctggagacc gaccgtgccg tgcgccacaa
     5041 tcgtgccctg cggcgccacg ctcgcgcgca gcgcgagcgc aatgggctcg cgcggtggaa
     5101 ccggcaccat cggcgcctcg agcgtggcct gtttcagctc gacgcccgca taggcaaggc
     5161 cgctgccctc gatgacgact tcgtggagag caagcggcgc cagcgacagc gcgccgcgcg
     5221 cggcgatccg gcctgcgccg ccggcgaggc gcccggcggc ttcgaagcgt ccgggctgcg
     5281 cctcgccggt cgatagatcg cgcgcgtgaa acgtgagctc ctgcagggcg agcccctgcc
     5341 cagcgacctg caccgtgcca ccttcgaccg cgagctcgcg cacgacccaa gcccggtgcc
     5401 acagagaagc cgaggaaagg tcgacgctcg ccgtccgggc cgcagcgttg cggcccagcg
     5461 ggccgcgcaa ctcgatgcct tccagcgcga cgctgagcgc gaaggggtga gtgcgcacgg
     5521 ttttcagctc gaggttgaat ccctgctttg ccgccgcgtc cacgagcgcc tcgcgcacgt
     5581 agcggggagc gatccaggtg ccggctgctg cgtacagggc gacgaggagc gccgtccccg
     5641 caccgaccgc caggatcttg cgcatgatcc cggtgcagca aaattcgcgc cggctacttc
     5701 ggagccgcct tcacctcgtc gccccacttc ttgaggaggc gattgcgctc agccgaggag
     5761 ccgtatttcg ccgagtcgta gtcgatcagc ttgatcgcgg cgatcttcgg cgcctgggca
     5821 tggaccggcg cggccttgtt tgacgggatg gagtagatgt tctggtccac gccgacgatc
     5881 ttctgcgcct gcgccgaaag ggtccaatcg aggaagcgct tcgcggcttc ggcgttcttg
     5941 cgccccttga tgagcgacac cgcgccgatc tcgtagccgg tgccctcgca gggcgcgacg
     6001 gtctggatcg gcgcgccctg cacgcgcatc gtcaccatgt cgtgcaggaa ggcgatgccg
     6061 accccggtct cgcccgacgc cgtcgacttg accggggcga tccccgactt ggtgtactgg
     6121 ttgacgttgc ggtgcagggc cttcaggtac gcgaaaccct tgtcctcgcc catgagctgc
     6181 acgatggtcg cgagcatcac gtaggccgtg cccgaggaat tcgggtccgc gatctgcacc
     6241 tcgtccctga atctcgcatc gaggaggtcc gcccagcact tcggctcggg aatgttcttc
     6301 ttcttcagca cgtcggtgtt gtagccgatg ccgagcgcgc cgaggtagat gcccgtggtc
     6361 ctgaatttcg acagctccgc gagccgcacc gcccacggat gaagctcggc gagcttcggc
     6421 gacttgtatt cctcgagcag gccttcctcc gcggcctgca tgtggggatc ccccgggccg
     6481 ccccaccaca cgtcgccgcg cgggctcgac gcctcggcgc gaatctgcgc gtaggtttcg
     6541 ccggtcccct tgcgcaccat cgtgaccttg gtgcccgtgg cgcgctcgaa cgcggccacc
     6601 ccggcccggc attgctcgtc gatgatcgag cagtaaacgg tcagcggcga ctgggcgccc
     6661 gcctggaacg acgtgaaaca gaacaacaag gcagcgatgt gcgcgagctt cattccccct
     6721 ccccgtcccg cctagattgc cataacccgc gcgagctcga gcaggctcgc gtcggcgggg
     6781 cgcgtgcgcc ccgtgatgtc ggcgagcggc gtggtgacga tgtcgttctt cacgatgccg
     6841 acgagcacgc cgtgccggcc cgcggccagc gtctcgatcg ccgccgcgcc gagccgcgtg
     6901 gcgaggacgc ggtcggcggc gccgggaatg ccgccgcgca ccacgtggcc caggcgcgtc
     6961 acacgcagct cgaagccgat gtcggcgcgg cgcgccgcgt agtactccat cagctcgtgc
     7021 acgccgcact tcgcgccctc ggcgatcacc gcgagcgcgt gggtcttgcc gcggcggtag
     7081 gcgtcgcgca gacgttcggc gacttgcgaa ggcaggatct cgctctcggg cagcgcgatc
     7141 acctcggcgc cgccggcgat gccggccatg agcgcgatgt agccgcagtc gcgccccatc
     7201 gtctccaccg cgaacgcgcg ctggtgcgac gacgccgtgg tgcgcaggcg gtcgatcgcc
     7261 tcgagcgtga tgttgatcgc ggtgtcgcag cctatgctca cgtcgttgcc gtagaggtcg
     7321 ttgtcgatag tggagggcac gccgactacg gcgaagccct cgcgcgcgag gctcgccgag
     7381 cccgactgcg agccgttgcc gccgatcacg accagggcgt cgattgattt tttcttgagg
     7441 tttgagagcg ctttggcgcg cccggcggcc tcgacgaact ccgggcagcg cgcgctgccg
     7501 agcacggtgc cgcccgactg cacgatgccg ccgacgtcgc gcgcggcgag cggctcgatc
     7561 aggtcgccga gcaggccggc gtagccgttg cgcacgccga agacttccca gccgcgggcg
     7621 agcgccccc
//
LOCUS       DATBSZ010000287         5340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2381696, whole genome shotgun sequence.
ACCESSION   DATBSZ010000287 DATBSZ010000000
VERSION     DATBSZ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2381696"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VIG70_18695"
     CDS             complement(<1..980)
                     /locus_tag="VIG70_18695"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY8252625.1"
                     /translation="MKTSEKICGMGHSMKRKEDPRFIRGKGNYVDDIQLPGMLYMDIV
                     RSPYAHAKIKKIDTSAALKIPGVLAVIDGPTLEKYKLHWMPTISGDTQMVLPIDEVMY
                     VAQEVCAVIATERYIAADGADAVEVEYEPLPVLVDPLKSIAPGAPVLRKDKKGKKDNL
                     AFHWEAGDRGATDSALGQADVVASQFVYVPRIHVASMETCGCVADFNSAEGKLTVYMT
                     TQAPHAVRTVFALVAGHVGLSEERIRIVSPDIGGGFGGKVPVYPGYVIAVAASVVIGK
                     PVKWIEDRMENLQADSFARDYHMKVELGAKKDGTMTALRIKTIADHGYTNA"
     gene            complement(977..1444)
                     /locus_tag="VIG70_18700"
     CDS             complement(977..1444)
                     /locus_tag="VIG70_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8252626.1"
                     /translation="MKHKISVTVNGAAREAEVESRLLLVHFIRENLRLTGTHIGCDTS
                     HCGVCTVLLDGQPVKSCTLLAVQADGCSVSTVEGLEQGGKLHPVQEAFTEKHALQCGF
                     CTPGMLMTCSALLERNKKPSEEEIRQAISGNLCRCTGYLNIVKAVQHAAEKMA"
     gene            complement(1446..2318)
                     /locus_tag="VIG70_18705"
     CDS             complement(1446..2318)
                     /locus_tag="VIG70_18705"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY8252627.1"
                     /translation="MIPAPFDYHRPGTLDEAIGLLARYGDQAKVLAGGMSLLPTLKLR
                     LGSYAHLVDINRIPGLEYVKEEKGWLRIGAGTRQAALERSELVKTKYPLLADAIPLIA
                     DPLVRNRGTIGGNIANGDPANDWPAIVIALGAELVAKGRGGERTIPASRFYKGLFVTD
                     LKADEILTEVRIPAPAGQSGGAYRKLKRKTGDYAVVAVAVQLALGKGGAVERAGVALT
                     NVGPTPTDAVAAAKFLAGKTLDEKTIAEASKLAAAAGSPSGDRRGSSDYKREMTRVLA
                     GRALKAALERAGRS"
     gene            complement(2315..3184)
                     /locus_tag="VIG70_18710"
     CDS             complement(2315..3184)
                     /locus_tag="VIG70_18710"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY8252628.1"
                     /translation="MRPELLPLAASLSAKGERFALVTVVRREPPSSARVGDTALVTES
                     GEYHGWAGGGCTRETVVREAQRAMADGEPRLLCLSPETNPTTRSGMIALQMTCDSGGT
                     VEIYVEPVLPVPRLVLFGATPMVEALARIGEAMGYRVERGADAALKGAHVLVGTMGDE
                     DLEVLQKVLRAEPAYVGVIASRKRFDHVRQTLIACGVPRAAVERVAAPAGLDIGARTP
                     EEIALSVMAQIVERSRKAAPVETVLEEAIDPICGMAVAVAGARHSAELEGKTYYFCCA
                     GCRTKFLQKGVSA"
     gene            complement(3265..4221)
                     /locus_tag="VIG70_18715"
     CDS             complement(3265..4221)
                     /locus_tag="VIG70_18715"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY8252629.1"
                     /translation="MHIKTAVLVSTIAVSLLGVAAHAQQPQQTCFPSKHGKDDQVGNL
                     NYVTPEKTLAAAKLVTKGKSYRLGIETNKDTPAFPPRTWSITILQPGQVHGASLGPTK
                     TTYNDDIITGWVGIGSQLDGLGHIGIDGVYYNCNKAAEFVQADGLKKLGVEKVPPIAT
                     RGVLLDMAGYFNTDIVKEGTAFNRAEIEGAMKRQGVKSIERGDVVLFHTGWTKLIGKD
                     NKRYGSVEPGLGVGGARYLAERQVAMVGADTWGVEVVPFEKNQGVFEVHQILLPMNGI
                     YILENMETSEMVKDQAWEFLFTLGPSRITGAVQAIINPIAIK"
     gene            4418..5029
                     /locus_tag="VIG70_18720"
     CDS             4418..5029
                     /locus_tag="VIG70_18720"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEY8252630.1"
                     /translation="MQAEIFQATSSFTNCGLPGVAQVPYGTHMCHFYRERADMVAALV
                     PFFVAGLNSNERCIWITAEPLNAADARLELQKTGVDVDAAIRSGALTVRDFSAWYTDS
                     AGLKSSEVAELWLAEEERALAEGYSGLRITGNASFLTPETWPDFMDYEEIVHRAFHGR
                     RILSLCSYDARRCSAADIREVVRRHSCALAQPDEGWQVVTPTV"
     gene            complement(5037..>5340)
                     /locus_tag="VIG70_18725"
     CDS             complement(5037..>5340)
                     /locus_tag="VIG70_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8252631.1"
                     /translation="RERKVLTLEEAVRRMTSDPADFFGIKDRGRIAPGMAADLVIFDP
                     ASVGSGGRPERLYDLPGGAKRMVMRSRGIEMTLVNGVVTWEKGALTGAAAGRVLRS"
BASE COUNT          795 a         1871 c         1701 g          973 t
ORIGIN      
        1 gcgttggtgt agccgtggtc ggcgatggtc ttgatgcgca gcgccgtcat cgtgccgtcc
       61 ttcttcgcgc cgagctcgac cttcatgtgg tagtcgcgcg cgaacgaatc ggcctgcagg
      121 ttctccatcc ggtcctcgat ccacttcacc ggcttgccga tcaccaccga cgccgccacc
      181 gcgatcacgt agccgggata gaccggcacc ttgccgccga agccgccgcc gatgtccggc
      241 gaaacgatcc ggatcctttc ctccgacagg ccgacatggc ccgcgaccag cgcgaacacc
      301 gtgcgcaccg cgtgcggcgc ctgcgtggtc atgtacaccg tgagcttgcc ctcggcgctg
      361 ttgaagtcgg cgacgcagcc gcaggtctcc atcgaggcga cgtggatgcg cggcacgtag
      421 acgaactggc tcgccaccac gtcggcctgg ccgagcgcgc tgtcggtggc gccgcggtcg
      481 cccgcctccc agtggaaggc gaggttgtcc tttttgccct tcttgtcctt gcgcagcacc
      541 ggcgcgcccg gcgctatcga tttcagcgga tcgaccagga cgggcagcgg ctcgtattcc
      601 acctcgactg cgtcggcgcc gtcggccgcg atgtagcgct cggtggcgat caccgcgcac
      661 acctcctgcg ccacgtacat cacctcgtcg atcggcagca ccatctgcgt gtcgcccgag
      721 atggtcggca tccagtgcag cttgtacttc tcgagcgtcg gcccgtcgat caccgccagc
      781 acacccggga tcttgagcgc ggccgaggtg tcgatcttct tgatcttggc gtgcgcatag
      841 ggactgcgca cgatgtccat gtacagcatc cccggaagct ggatgtcgtc gacgtagttg
      901 cccttgccgc ggatgaagcg cgggtcctcc ttgcgcttca tcgagtgacc catgccgcag
      961 atcttctcgc tggttttcat gccatcttct ccgcggcgtg ctgcaccgct ttcacgatat
     1021 tgaggtagcc cgtgcagcgg cacaggttcc cggagatcgc ctggcggatc tcttcttcgc
     1081 tcggcttctt gttgcgctcg aggagcgccg agcaggtcat cagcattccc ggcgtgcaga
     1141 agccgcactg gagcgcgtgc ttctccgtaa acgcttcctg caccgggtgg agcttgccgc
     1201 cctgctccag gccctcgacc gtggagacgc tgcagccgtc ggcctgcacc gcgagcagcg
     1261 tgcacgactt caccggctgt ccgtcgagca gcaccgtgca cacgccgcag tgcgacgtgt
     1321 cgcagccgat atgggttccg gtaaggcgca ggttttctct tatgaagtgc accagcagca
     1381 atcgcgattc cacttccgct tcgcgcgccg cgccgttcac cgtgacgctg atcttgtgct
     1441 tcatctcagc tcctccccgc gcgctcgagc gcagccttca gggcccgccc cgccagcacg
     1501 cgcgtcatct cgcgcttgta gtcggacgag ccgcggcggt cgccgctcgg cgagccggcc
     1561 gcggcggcga gcttcgacgc ttccgcaatg gttttttcat ccagggtctt gcctgcgagg
     1621 aatttcgcag ccgcaaccgc atccgtcggc gtcggcccca cgttggtcag cgcgacgccg
     1681 gcgcgctcca ccgcgccgcc cttgccgagc gcgagctgca ccgcgaccgc gaccacggcg
     1741 tagtcgccgg tcttgcgctt gagcttgcgg tacgcgccgc cgctctgccc cgcgggcgcc
     1801 ggaatgcgca cctcggtgag gatctcgtcg gccttgaggt cggtgacgaa caggcccttg
     1861 tagaagcggc tcgccgggat ggtgcgctcg ccgccgcgcc ctttcgccac cagctcggcg
     1921 ccgagcgcga tgacgatcgc cggccagtcg ttcgccgggt cgccgttggc gatattgccg
     1981 ccgatggtgc cgcggttgcg caccagcggg tcggcgatga gcggaattgc atcagccaac
     2041 aggggatact ttgttttgac caattcactt ctctcgaggg cggcctgccg cgttcccgcg
     2101 ccgatgcgca gccagccctt ctcttccttg acgtactcga gcccgggaat ccggttgatg
     2161 tcgaccaggt gcgcatagga gcccaggcgc agcttgagcg tcggcagcag gctcatgccg
     2221 cccgcgagca ccttggcctg gtcgccgtag cgcgcgagca ggccgatggc ctcgtcgagc
     2281 gtccccgggc ggtgatagtc gaaaggcgcg ggaatcatgc gctcactcct ttttgaagga
     2341 acttggtgcg gcagcctgcg cagcagaagt agtaggtctt gccctcgagc tccgcgctgt
     2401 ggcgcgcgcc ggccacggca accgccatgc cgcagatcgg gtctatggct tcctctaaaa
     2461 cagtctcaac aggggcagct tttcggctcc gctccacgat ctgcgccatg acgctcagcg
     2521 caatctcttc cggcgtccgc gcgccgatgt ccagccccgc gggcgccgcg acgcgctcca
     2581 ccgcggcgcg cggcacgccg caggcgatca gggtctggcg cacgtggtcg aagcgcttgc
     2641 ggctcgcgat cacgccgacg taggccggct ccgcgcgcag gaccttttgc aggacttcca
     2701 ggtcctcgtc gcccatggtt cccaccagca cgtgcgcgcc cttgagcgca gcatccgccc
     2761 cgcgctcgac gcggtagccc attgcctcgc cgatgcgcgc gagcgcctcg accatgggcg
     2821 tggcgccgaa caggaccagg cgcggaacgg gcagcacggg ctcgacgtag atttccacgg
     2881 tgcctccgct gtcgcaggtc atctggaggg cgatcattcc tgatcttgtc gttgggtttg
     2941 tctcagggct caaacacaac aggcggggtt caccgtcggc catcgcgcgc tgcgcctcgc
     3001 gaaccaccgt ctcgcgcgtg cagccgccgc ccgcccagcc gtggtattcg ccgctctcgg
     3061 tgaccagcgc cgtatcgccc acgcgtgccg agctcggcgg ctcgcgccgc accacggtga
     3121 cgagcgcgaa gcgctcgccc tttgcgctca gacttgctgc cagcggcaat agctcgggcc
     3181 gcatatcccc ctccaagaga cgcaggcgat actagcgcat acaccgccgc cggagcgcga
     3241 attcccgctt atcgcgccgt ccgttcactt gatcgcgatc ggattgatga tcgcctgcac
     3301 cgcgccggtg atgcgcgaag gcccgagcgt gaagagaaac tcccacgcct ggtctttcac
     3361 catctccgaa gtctccatgt tctcgaggat gtagatgcca ttcatgggca gcaggatctg
     3421 gtgcacctcg aacacgccct ggtttttctc gaacggaacc acctcgaccc cccaggtgtc
     3481 ggcgccgacc atcgccacct ggcgctcggc gaggtagcgc gcaccgccca cgccgaggcc
     3541 gggctcgacc gagccgtagc gcttgttgtc cttgccgatc agcttggtcc agccggtgtg
     3601 gaacagcacg acgtcgccgc gctcgatcga cttcactccc tggcgcttca tcgcgccctc
     3661 gatctcggcg cggttgaagg cggtgccttc cttcacgatg tcggtattga agtagcccgc
     3721 catgtcgagc agcacgccgc gcgtcgcgat cggcggcacc ttctcgacgc cgagcttctt
     3781 caggccgtcg gcctggacga actcggcggc cttgttgcag ttgtagtaga caccgtcgat
     3841 gccgatgtgg cccaggccgt cgagctgcga gccgatgccg acccagccgg tgatgatgtc
     3901 gtcgttataa gtcgtcttcg tcgggccgag cgaggcgccg tgcacctggc ccggctgcag
     3961 aatggtgatc gaccaggtgc gcggcggaaa cgccggcgtg tccttgttgg tttcgatgcc
     4021 gaggcggtag ctcttgccct tcgtcaccag cttcgccgcg gcgagcgttt tctccggcgt
     4081 cacgtagttc aggttgccga cctggtcgtc cttcccgtgc ttggacggaa agcacgtctg
     4141 ctgcggctgc tgcgcgtgcg cggcgacgcc gaggagcgac accgcgatcg tcgaaacgag
     4201 taccgctgtc ttgatgtgca tggcctctcc cggttaggac cggggaagag gctacgtcgc
     4261 gcgttcgccg tgcgtcaatc agcgtcgggt gatgggtcgg tgcactccag cggtgcgggg
     4321 gagccacaac cggataaaaa ccgccgcgaa actctgagaa gttgtaaggc cctccccgct
     4381 agcgcacgct ggggcgctcg gcctaggatt tgcagatatg caggccgaga ttttccaagc
     4441 gacgtcgagc ttcaccaatt gcgggctgcc cggggtcgcg caggttccgt acggcaccca
     4501 catgtgccac ttctaccgcg agcgcgcaga catggtggcg gcgctggtgc catttttcgt
     4561 cgcgggattg aacagcaacg agcgctgcat ctggatcacc gccgagcccc tgaacgccgc
     4621 cgatgcccgg ctggagctgc agaaaaccgg tgtcgacgtg gacgcggcga ttcgaagcgg
     4681 agcgctcacg gtgcgcgact tttctgcgtg gtacaccgac tccgcgggac tgaagagcag
     4741 cgaggtcgcg gagctctggc tcgccgagga ggaacgcgcc ctggcggaag gctacagcgg
     4801 gcttcgcatc accgggaacg ccagcttcct cacgccggaa acctggcccg acttcatgga
     4861 ctacgaggag atcgtgcacc gggcgttcca cggccggcgc atcctttcct tgtgcagcta
     4921 cgacgcccgc cgctgctccg ccgccgatat tcgggaagtc gtgcgccgtc acagctgcgc
     4981 gctcgcccag cccgacgagg gctggcaggt cgtcaccccg accgtctagc ggcgaatcag
     5041 gaacgcagca cgcgtcccgc cgcggcgccg gtgagcgcgc ccttctccca ggtcaccacg
     5101 ccgttcacca gcgtcatctc gatgccgcgc gagcgcatca ccatgcgctt cgcgccgccc
     5161 ggcaggtcgt agagccgctc gggacgcccg ccggagccaa cgctcgccgg gtcgaagatc
     5221 accaggtccg cggccattcc cggcgcgatc ctgccgcgat ccttgatgcc gaagaagtct
     5281 gccggatccg acgtcatccg ccgcaccgct tcttcaagag tgagcacttt gcgctcgcgc
//
LOCUS       DATBSZ010000288         4876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1287143, whole genome shotgun sequence.
ACCESSION   DATBSZ010000288 DATBSZ010000000
VERSION     DATBSZ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287143"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VIG70_18730"
     CDS             complement(<1..472)
                     /locus_tag="VIG70_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEY8252632.1"
                     /translation="MYVGRFAPSPTGPLHFGSLVAALASWLDARAAGGRWLVRIEDLD
                     APRNQPGAAQDILRTLERIGLAWDGEVAYQSQRTGRYREALGRLEKQTYWCACSRREI
                     ADSSLGLATDGAQIYPGTCRNGIAPDRTPRALRLRTSAERIVFDDLLQGRQQQIL"
     gene            527..922
                     /gene="trxA"
                     /locus_tag="VIG70_18735"
     CDS             527..922
                     /gene="trxA"
                     /locus_tag="VIG70_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY8252633.1"
                     /translation="MATVELTKDNFEKTVNENPMVIVDFWAPWCGPCRGFAPVYEKAS
                     EAHADVVFAKVNTEEEQELAGAFGIRSIPTLMVFREKVILFQQAGALPGPALEQIITQ
                     AKTIDMTKVHAEVEGQRQGRDQAQEQDQK"
     gene            936..2330
                     /gene="fumC"
                     /locus_tag="VIG70_18740"
     CDS             936..2330
                     /gene="fumC"
                     /locus_tag="VIG70_18740"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY8252634.1"
                     /translation="MSGSRLEKDSFGTIEVPAERLWGAQTERSRRFFRISGERMPPPV
                     IYALALVKKAAAAVNAELNLLSKQKAEAIQRAAEEVLAGRHDAEFPLVVWQTGSGTQT
                     NMNVNEVLANRASELLGGERGQKRLVHPNDEVNLGQSSNDVFPTAMHIAAARALQEEV
                     LPAAEALKATLAKKRDAFAAIIKIGRTHLQDATPLTLGQEFSGYVAQLEHAQAALRAV
                     LPPLYELAIGGTAVGTGLNTHPEFAGRVCAAVASHTKLAFVEASNKFAALGGHEPLVF
                     AHGALKTLSTALSKIANDIRLLSSGPRSGLAELRIPENEPGSSIMPGKVNPTQAEALT
                     MLCAQVAGNDVAVGIGAASGHLELNVYKPMIIHAFLQSARLLADGCRSFDEHCARGIE
                     PDQARIRELLERSLMLVTALAPHIGYDRAAEIAKKAHREGSTLREAALALGYVSAQDF
                     DRWVRADAMIGPRR"
     gene            2327..3202
                     /locus_tag="VIG70_18745"
     CDS             2327..3202
                     /locus_tag="VIG70_18745"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY8252635.1"
                     /translation="MKRRALLTALLLAALPARAQARRFERGLLWRVEKKGVAPSHVYG
                     TIHVADARLAELPAMVRQRFDAAKSLMLEFVPDPYSRERFLEASMFLDRQTLEEKIGA
                     QDFERALEVLAPIGLPRDVVNKLKPWGVLLNLRNPMRAERGTPLDMHLAELARARRLP
                     LSQIEGVEEQIFTFDEFPMESQVALLKHSLAHRDELTELAERTLEAYLERDLAAIWRL
                     REQFAARHPQIAAHQAAMTKRVVHDRSVVMAFRMQRQLRRGEAFVALGALHLYGDKGV
                     LALLEGDGYRAERVF"
     gene            3204..3827
                     /gene="purN"
                     /locus_tag="VIG70_18750"
     CDS             3204..3827
                     /gene="purN"
                     /locus_tag="VIG70_18750"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY8252636.1"
                     /translation="MVVILISGRGSNMQALIDAGLPVSAVISNRGDAAGLALAAARGI
                     ATRVVEHRRYATREEFEAALAAEIDRFAPRLVALAGFMRIFTPVFVARYRDRLLNIHP
                     SLLPAFPGLHTHERALAAGVKLHGCTVHFVTADLDHGPIVAQAAVPVRAGDAAATLAS
                     RVLKQEHVIYPRAVRWFLDGRLVLGNGVVHLNQNAAGNDAQLVLAAD"
     gene            3799..4488
                     /locus_tag="VIG70_18755"
     CDS             3799..4488
                     /locus_tag="VIG70_18755"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY8252637.1"
                     /translation="MRNSFWLLISLPVALSALAQVLPPARVELEYDLMRNGSTMAEVV
                     ERLEHGNGTYHLTETWKGRGVYALLGRAKRTSEGDLGADGPRPREYVDERSGRDTQRV
                     SFDWSANTITRRYKGATRTEPVPADTQDRLSFLFALTFYAQKGEPVSFHIADGRGMSR
                     HTYRPNGRERLATPAGEFDTVKLLRKNEGSGEVSEIWLAADRGYLPVRISVVEKDGTR
                     YEHVATRISQP"
     gene            4485..>4876
                     /locus_tag="VIG70_18760"
     CDS             4485..>4876
                     /locus_tag="VIG70_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8252638.1"
                     /translation="MRITRAVLAHARAALSMILRFEAPADQCLSRYFRDHRNLGQSER
                     AFVAETVFAVLRRKRSLEAAAGSPEPQALIAAALLRVHGLSARALDGLVDEDLSRRVR
                     RLQIAALPAAVRADLPDWLWERLAQQHG"
BASE COUNT          792 a         1692 c         1659 g          733 t
ORIGIN      
        1 cgaggatctg ctgttgacgg ccctggagga gatcgtcgaa cacgatgcgt tccgcggagg
       61 ttctcaatcg aagcgcacgc ggcgtcctgt ccggcgcgat cccgttgcgg caggtgccgg
      121 gatagatctg cgcgccgtcg gttgcgaggc cgagcgatga atcggcgatc tcgcggcgcg
      181 agcaggcgca ccagtaggtc tgcttttcaa gccgtcccag cgcttctcgg taccggcctg
      241 tgcgctggct ttgatacgcg acctcgccgt cccaggcgag gccgatccgc tcgagagtac
      301 gcagaatatc ttgcgccgcg ccgggctggt tgcgaggcgc gtcgaggtcc tcgatgcgca
      361 ccagccagcg gccgcccgcg gcgcgcgcat cgagccagct tgcgagcgcc gcgaccagcg
      421 acccgaaatg cagcggaccg gtgggcgagg gcgcgaaccg gccgacgtac atgattgtct
      481 agagtacact gccccccgtt tttactgacc cgccgaaagg actgtcgtgg ccaccgtcga
      541 gctcaccaag gacaacttcg agaagaccgt caacgagaac ccgatggtga tcgtcgactt
      601 ctgggcgccc tggtgcgggc cgtgccgcgg ctttgcgccg gtctacgaga aggcctcgga
      661 agcccatgcg gacgtggtgt tcgccaaggt gaataccgag gaggagcagg agctcgccgg
      721 cgccttcggt atccgctcca tcccgacgct gatggtgttc cgcgagaaag tgatcctgtt
      781 ccagcaggcg ggcgctctgc ccggcccggc gctcgagcag atcatcacgc aggcgaagac
      841 catcgacatg acgaaggtgc acgccgaggt ggaaggtcag aggcagggcc gggatcaggc
      901 ccaggagcag gaccagaagt aacattaggg ccgcgatgag cggctcacgc ctcgagaagg
      961 acagcttcgg caccatcgag gtgcccgccg agcggctgtg gggcgcgcag accgagcgca
     1021 gccgccgctt cttccgcatc tcgggcgagc gcatgccgcc cccggtgatt tacgccctgg
     1081 cgctggtcaa gaaggcggcg gccgcggtca atgcggaact gaaccttctt tcgaaacaaa
     1141 aagcggaagc gattcagcgc gccgcggaag aagtgctcgc cggccgccac gacgccgagt
     1201 tcccgctggt cgtctggcag acggggtcgg gcacgcagac caacatgaac gtgaacgagg
     1261 tgctggccaa tcgcgcctcc gagctgctcg gcggcgagcg cggccagaag cgcctggtgc
     1321 atccgaatga cgaggtcaac ctcggccagt cgtccaacga cgtctttccg accgcgatgc
     1381 atatcgccgc cgcgcgtgcg ctgcaggagg aggtcctccc cgccgccgag gccttgaaag
     1441 cgacgctcgc gaaaaagcgc gacgccttcg ccgcgatcat caagatcggg cgcacccacc
     1501 tgcaggatgc gacgccgctc accctgggcc aggagttctc cggctatgtc gcccagctcg
     1561 agcacgccca ggccgcgctg cgcgcggtgc tgccgccgct ctacgagctg gcgatcggcg
     1621 gcaccgcagt cggcactggg ctcaacacgc atccggagtt tgcgggcaga gtatgcgcag
     1681 ccgttgcttc gcacaccaag ctggctttcg tcgaggcaag caacaaattc gccgccttgg
     1741 gcgggcatga gcctctggtg ttcgcgcacg gcgcgctgaa gaccctctcc accgcgctgt
     1801 ccaagatcgc gaacgacatc cggctcctca gctccgggcc gcgctccggg ctcgccgagc
     1861 tgcggattcc ggaaaacgag cccggcagct cgatcatgcc gggcaaggtc aaccccaccc
     1921 aggccgaggc gttgacgatg ttgtgcgcac aggtggccgg gaacgacgtc gcagtgggga
     1981 tcggcgcggc ttccggccac ctcgagctca acgtctacaa gccgatgatc atccacgctt
     2041 tcctgcagag cgcgaggctg ctcgccgacg gctgccgctc tttcgacgag cattgcgcgc
     2101 gcggcatcga gcccgaccag gcgcgcatcc gcgagctgct cgagcgctcg ctcatgctgg
     2161 tgaccgcgct cgcaccgcat atcggctatg accgcgctgc ggagatcgcg aagaaggcgc
     2221 accgcgaagg cagcacgctg cgcgaggcgg cgctcgcgct cggctacgtc agcgcacagg
     2281 acttcgaccg ctgggtgcgc gccgacgcga tgatcgggcc ccggcgatga aacgcagggc
     2341 cttgttgacg gcgctgctgc tcgcagcgct tccggcgcgc gcgcaggccc gccgcttcga
     2401 gcgtggattg ctctggcgcg tcgaaaagaa aggcgtcgca ccgagccacg tctacggcac
     2461 gatccacgtc gccgacgcaa ggctcgccga gcttccggcc atggtgcgcc agcggttcga
     2521 tgcggcgaag agcctgatgc tcgaattcgt gcccgacccg tactcgcggg agcgtttcct
     2581 cgaagcctcc atgttcctcg atcgccagac gctcgaggag aagatcggcg cgcaagactt
     2641 cgagcgcgcg ctcgaggtcc tcgcaccgat cggcctgccg cgcgacgtgg tcaacaagct
     2701 caagccctgg ggcgtgctcc tgaacctgcg caatcccatg cgcgcggagc gcgggacgcc
     2761 gctcgacatg catctcgccg agctggcgcg cgcgcggcgg ctgccgctct cgcagatcga
     2821 gggcgtcgaa gagcagatct ttaccttcga cgagttcccg atggagtcgc aggtggcgct
     2881 gctcaagcac agcctcgcgc accgcgacga gctcaccgag ctcgccgagc ggacgctgga
     2941 ggcctacctc gagcgcgatc tggccgcgat ctggcggctg cgggagcagt ttgccgcgcg
     3001 ccacccgcag atcgcggcgc accaggcggc gatgacgaag cgggtggtgc acgaccgcag
     3061 cgtggtgatg gcgttccgca tgcagcggca actgcggcgc ggcgaagcct tcgtcgcgct
     3121 cggcgcgctg cacctctacg gcgacaaagg cgtcctcgcc ctgctggaag gtgatggcta
     3181 ccgcgccgaa cgggtatttt gacgtggtcg tcatcctcat ctcagggcgc ggcagcaaca
     3241 tgcaggcgct gatcgacgca ggcctgccgg tgagcgctgt tatcagcaac cgcggcgacg
     3301 ctgcggggct ggcgctcgcc gcggcgcgcg gcatcgcgac ccgggtggtc gagcatcggc
     3361 gttacgccac gcgcgaagag ttcgaggccg cgctcgccgc cgagatcgac cggtttgcgc
     3421 cgcgcctcgt ggcgctcgcc ggcttcatgc gtatattcac gccggtgttc gtcgcccgct
     3481 accgtgatcg cctgctcaac atccaccctt cgctcctgcc cgcgttccca ggcctgcaca
     3541 cgcacgagcg tgccctcgcc gccggcgtca agctgcacgg ctgcacggtc catttcgtga
     3601 ccgccgatct cgatcacggt ccgatcgtcg cgcaggcggc ggtgcccgtg cgcgccggcg
     3661 acgctgccgc gacgctcgcc tcgcgcgtgc tgaagcagga gcacgtcatc tacccgcgcg
     3721 cggtgcgctg gttcctcgac ggccggctgg tcctcggcaa cggcgtggtt cacctaaacc
     3781 aaaacgctgc aggcaacgat gcgcaactcg ttctggctgc tgattagcct gccggtcgcg
     3841 ctttcggcgc tcgcgcaggt gctcccgccg gcacgggtcg agctcgaata cgacctcatg
     3901 cgcaatggca gcacgatggc cgaggtcgta gagcgcctgg agcacggcaa cggcacctac
     3961 cacctcaccg agacctggaa agggcggggg gtctacgcgc tgctcgggcg ggcgaagcgc
     4021 acgagcgaag gcgatctcgg agccgacggc ccgcggccgc gggaatacgt cgacgagcgc
     4081 agcggccgcg acacgcagcg cgtctcgttc gactggagcg cgaacacgat cacgcgccgc
     4141 tacaagggcg cgacgcgcac cgagccggtg cccgcggata cgcaggaccg gctgtcgttc
     4201 ctcttcgcgc tgacctttta cgcgcagaaa ggcgagccgg tcagcttcca catcgccgac
     4261 ggccgcggca tgtcgcgcca tacctaccgg cccaacggcc gggagcgcct cgcgacgccc
     4321 gccggcgagt tcgacacggt gaagctcctg cgcaagaacg agggctcggg cgaggtgtcg
     4381 gagatctggc tcgccgcgga ccgaggctac ctgccggtga ggatctccgt cgtggagaag
     4441 gacggcacgc gctacgagca cgtcgctacg cgaatttccc agccgtgaga attacgcgcg
     4501 cggttctggc gcatgcccgc gccgcgcttt ccatgatcct gcgcttcgag gcgccggccg
     4561 accagtgcct gtcgcgctat ttccgcgacc atcgcaacct cgggcaaagc gagcgcgcgt
     4621 tcgtcgccga aaccgtgttc gccgtgctgc gccgcaagcg ctcgctcgag gcggcggccg
     4681 gctcgcccga gccgcaggcg ctgatcgccg cggcgctgct gcgcgtccac ggactatcgg
     4741 cgcgcgcgct cgacggtctt gtcgacgagg atctcagccg ccgagtccgc cggctgcaga
     4801 tcgccgcgct gccggccgca gtgcgcgccg acctgcccga ctggctgtgg gagcgcctcg
     4861 cgcagcagca cggcca
//
LOCUS       DATBSZ010000289        23751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1014528, whole genome shotgun sequence.
ACCESSION   DATBSZ010000289 DATBSZ010000000
VERSION     DATBSZ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23751
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014528"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VIG70_18765"
     CDS             <1..510
                     /locus_tag="VIG70_18765"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8252639.1"
                     /translation="LPPEELMRKWLHVLRSAQRHALQIPAAKMHERATAGRDRSTLTL
                     AYHVYQIPDAFLQAIQDGVPDMAKVYDTPLPAAVKTTADVIAYGSRMEKKLEAWWKAL
                     PDKSCQQKVKTYYGDRPMHELLERCTWHSAQHARQIIAVLERLGVEPQGRLGAADYAG
                     LPMPKGLWE"
     gene            510..1541
                     /locus_tag="VIG70_18770"
     CDS             510..1541
                     /locus_tag="VIG70_18770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8252640.1"
                     /translation="MDKEKSRQALEKVFRDMAGAMTAGMVYVGVKTGLFRLMAGKGPL
                     TASDVTRISGLMPRYVEEWLKGMVAAGYLDFDPMAQTYALPEEHAYFLASEDSDHFVG
                     GLFAMVPPLMRVAPEVARAFTEGGGVRFEDFGADCVSALDLINSGQYESRFADYWMKS
                     LPEAAARLHAGGRALDVGCGSGRVCTALASAFPAAEIVGVDPDEASIERARSLSPKAR
                     FVAGDVSSVQGAFDFISLCDVLHDLAEPLQTLRQIRALLKPDATLFIVEPKAADRLED
                     NRNPVAATFYGFSLFHCMTQSLARGGPGLGTCLGPAATEALVRQAGYTRFRRLDIKSL
                     TNLFYSAQP"
     gene            1538..1882
                     /locus_tag="VIG70_18775"
     CDS             1538..1882
                     /locus_tag="VIG70_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY8252641.1"
                     /translation="MILKLALVWLINAVALIAVAYLMPSVTVSSFGAALAAALALGLI
                     NAVVRPVLVLLTLPVTLLTLGLFIFVLNGLLFWMVGAWLQGFEVGGFWSGVFGAILFS
                     LVSWALSALVLK"
     gene            1879..2130
                     /locus_tag="VIG70_18780"
     CDS             1879..2130
                     /locus_tag="VIG70_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252642.1"
                     /translation="MTFKQALHRHLQALDRIKEHARLFATPVEIHESADAALAVLHLL
                     YCEDAPGKRPLRSQSHLTLVYQRRGAEWVVVQDRHCPIS"
     gene            2091..3179
                     /locus_tag="VIG70_18785"
     CDS             2091..3179
                     /locus_tag="VIG70_18785"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HEY8252643.1"
                     /translation="MGRGPGPALPDLLGPKSLLRRLARRWRDPIIILPYLTYGTVQKL
                     TVCGRVLEDEGFLPAADADTRWRNLMRFYKRLESDEVPGARLRARFLGVATEGVSDDE
                     GYFRLELAPRGRVGPGAWQEVELELLEARSIKATARVLVPSKRARFGVISDIDDTIVS
                     THVANKLRMILTVALSNARTRKPFLGVAAFYRALHAGVNPFFYVSKSPWNLYAPLVEY
                     LEAQGLPLGPLMLRDFGLSSNKDHKSEAIVDILRTYPKLKFILIGDSGEQDPEIYTRI
                     VQRFPERIRAIYIRSVNPKRIVEIEKLASEVARTGCQLVLAAEAEPAAAHAAAEGLIQ
                     ASELRAVRAEKDSEMSSSKPAVSSGALK"
     gene            complement(3089..5710)
                     /locus_tag="VIG70_18790"
     CDS             complement(3089..5710)
                     /locus_tag="VIG70_18790"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8252644.1"
                     /translation="MFEILLLVAASGGAVYLAMHVLSRRGEKALLDAKADSARFRGLT
                     ELSADWFWETDAEHRIVWLSGGAPVATFFGQTPTFGKRIWEIPGVEVEPRALEAHLEQ
                     LAARQSFFDLEIARVDERGARQVHIISGQSRLAADNRAFLGYRGVARDVTEQRSAERA
                     LFRAKERLELALDGGNLAEFHFDAERGELSAGDGWVRFLGHTASPTVTLGAELIAMMH
                     PDDRVHYTEALVRALKGEAPALDVEFRIRTRQGKWKWLHARGRVTEQGSHGRATRVSG
                     TVADIDERKRAEAALAEREQRFRDVVDASGEYVWETDAQGRYTYLSERAEAVLGYARA
                     ELLGRKPRDFMPLGEPRMVDEWFAGRAADGEPFRDLLHRMITKSGGVIWQSVSGVPVR
                     DPRARLIGWRGTGADVTARKHAEARIEQLTTRDALTGLPNSTLLADRASQAILTAARN
                     RSQFALLCIDLDRFKLVNESMGHRAGDALLRAVAERMGNLVHGEDTLARLGGDDFVLV
                     QSIKPSEDAAAAAAALAQRILGLLARAFTVESKTLSVAASIGISIYPNDGRDFVELLK
                     NADAALYHAKESGKGTWRLFAPALNARAAERLRLENELRSALARGELALHWQPVVRGW
                     PPDRRSSPLHWRGRVVGAEALVRWQHPTRGLLTPEHFVPLAEECGLIRAVDEWVLERA
                     VSQAGAWQRRYGHKLWTAVNVSAPQLSQGAAYVEKLKSTLREHQLEGRQVELEVTERV
                     LMSGLAENVATLKSIGELGVRFAIDDFGTGYSSLAYLRSLPIDKLKIDRSFLSGIDSQ
                     AADAAIVRAITALASTLGIAVAGEGVETAAQLEHLMALGCEEWQGHYFSAPLDTAGFE
                     ELISESFSARTARSSEA"
     gene            complement(5717..7531)
                     /locus_tag="VIG70_18795"
     CDS             complement(5717..7531)
                     /locus_tag="VIG70_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY8252645.1"
                     /translation="MQQVVKTPEGKTQSGARLTLAEVLDWLVADKMAEPEAAEKLKKE
                     RRYYRGTMHPLVIVADQKWKKGATAPGSALLTLDALTEWLAKRSSLEYLHIDPLKIDF
                     TGVTEIMSSAYATRFRILPVALNSKEAVIATAEPGIRDWEPELARMIKREIRRVIANP
                     LDIERYQVEFYNLAKSIKGANKNTSISGLSNFEQLVELGKSDKLDANDAHVVRVVDWL
                     WQYAFEQRASDIHVEPRRDLGIVRFRIDGVLHQVYQIPASVLAAMTSRIKILARMDVV
                     EKRRPQDGRIKTRSAEQDIELRISTLPTAFGEKLVMRIFDPEVLVRDFSELGFSAEDQ
                     KRWKEMTDRPNGIVLVTGPTGSGKTTTLYSTLKSLATSEVNVCTIEDPIEMVEPAFNQ
                     MQVVSAIELGFAEGVRALMRQDPDIIMVGEIRDLETAEMAVQSALTGHLVLSTLHTND
                     APSAIARLIELGVAPYLLNATLNGVMAQRLVRTLCPHCKQTVELNRAEDQDAWNALVA
                     PWKSKHPARLYKPVGCLECRMTGYMGRVGIYETLVFSQALKGLVSQNADLVALREQAF
                     KEGMKPLRISGAVKVGAGATTIDEVLKVAPPVRDAAAG"
     gene            7611..8885
                     /locus_tag="VIG70_18800"
     CDS             7611..8885
                     /locus_tag="VIG70_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8252646.1"
                     /translation="MRTAAKRALAPDVKLREVWAWSMYDFANSAYTTVVITAVFSAYF
                     VGVIAQNAPWSTFAWTAALSVSYAAILLTGPLVGAWADAHAAKKPLLLATTLGCVVFT
                     ALLYFTQPGAVAVALLLLIASNYFFGAGENLIAAFLPELADSRAMGRVSGWGWSFGYL
                     GGLTALGICLFYITSSEGKPASQTVPVTMLITAAFFALAAVPTFVFLHERAVPQARME
                     NPWARVRQTLRETERYADLRRFLVCLLFYQAGITAVVSLAAIYAEQAMKFTTQQTIAL
                     ILVVNVTAAIGAFGFGYLQDAVGHVRAVSLTLVGWIVMVVLAGLSESSASFWVAANLA
                     GLCMGSSQAAGRAVVGYLAPPARLAEFFGLWGLAVKAASIFGPLTYGAVTLIFAGNHR
                     LGIFVTGIYFVIGLILLRNIDVERGRAAALAA"
     gene            8897..9550
                     /locus_tag="VIG70_18805"
     CDS             8897..9550
                     /locus_tag="VIG70_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252647.1"
                     /translation="MRLFLAMLLAAAAAGTLAGPRGDTSEWSLNLFVVGSNHYAFEGG
                     ASARSDTGAGFGFSVARNLNDYFAVGVEGSLATFSYRASIAPGSGNGGAGFETRGDME
                     SAMLRVHATWNLLARPLTPFVTAAAGVIFLEPDFGAQPPADACWAYPWYGEVCGARAP
                     TSTLARLSYGAGAGLRFDFPRERGFIRAFVGGEWIEMSEAPSTVGYVTVRADFGLRF"
     gene            9599..11029
                     /locus_tag="VIG70_18810"
     CDS             9599..11029
                     /locus_tag="VIG70_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY8252648.1"
                     /translation="MRTLAPHFLSLVLLTAFATVAPAQAPEKQAAKSAAGGRTANLPD
                     FTALMKQHGPAVVNVITKREPARTARAPQEKQAPDDPLQEFFRRFMPDSPERAPGPGQ
                     GLGSGFIISQDGYVLTNAHVVAGDGEVTVRLADAKREFKAKVIGADERTDVALLKIDA
                     TGLPTVKLGKSASLQPGEWVAAIGSPFGFENTITAGIVSATGRSLPAETYVPFIQTDV
                     AVNPGNSGGPLINLAGEVVGVNSMIYSQTGGYMGVSFAIPIEVALDVSRQLRAEGKVT
                     RGRLGVRIQPMTKELAQSFKLNEPNGALIATVEPGSPADKAGLKPGDVVLAYNGQEID
                     DPNKLPRLVAATKPGQSATLKIWRGGKAEEVKFTAAELVADTRAAKPAQDKAIKPNRL
                     GLVVSELPPAQRRALGIEYGLVVESAEAGRTPLRPGDVIVGVGRESVKSVEDFDRLIG
                     EQKQGDTVALLVRRGEATVYVPVEVG"
     gene            11222..11632
                     /locus_tag="VIG70_18815"
     CDS             11222..11632
                     /locus_tag="VIG70_18815"
                     /inference="COORDINATES: protein motif:HMM:NF036497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY8252649.1"
                     /translation="MRSLVLLVLVFLSSCTPAPEGLRLVTIDNPERGLSLRELPPEAL
                     KSLGLPYGLAVVRAGSLAQRAGLKIGDVVYGVNQRKLKNLEELNQVLAEQEHGKLGLL
                     VRRGRSDFYVAVDLGGAPGVPRGLPAPRDTLLRT"
     gene            complement(11607..11849)
                     /locus_tag="VIG70_18820"
     CDS             complement(11607..11849)
                     /locus_tag="VIG70_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252650.1"
                     /translation="MKHMIFLALVSLAATALAQEKPAAKEPAKAEAMKEATKPEARQA
                     ATGPRMPHARRWHEDARHCLDLPSNTEIIKCAEEYL"
     gene            complement(11900..13198)
                     /gene="cls"
                     /locus_tag="VIG70_18825"
     CDS             complement(11900..13198)
                     /gene="cls"
                     /locus_tag="VIG70_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEY8252651.1"
                     /translation="MRRAKLHSSSNKWLIAGFTFAATALAVMLVANLSLGDKHIEQRV
                     ATLYSTADEQFARNMNVVFGPALVGGNRAQALVNGDRFFADMLKAIRSAQKTITFESY
                     IYWSGETGAEFTQALIERAQAGVKVHLLFDALGSGKIDDKSVKAMKAAGIEVEKYNPW
                     RWNTLARINNRTHRKIMVVDGRIGYTGGAGIGDEWRGDAQDPKHWRDTHFRIEGPVVA
                     QVQAAFIENWIEVTGRVLHGEEYFPALPRAGTQAAQFIVTSPGGGSESMQLMYLMSIA
                     AAAKSIRLSAAYFVPDGVEVRTLVAAAKRGVRVQLIVPGPHTDSPLVRRASRSTWGEL
                     LRAGIEFYEYQPTFFHCKVMVVDDLWVSVGSTNFDARSFSVNDEANLNVYDRDFAAAQ
                     ARIFEADLRRSKRVSLEDWENRPWTEKLWEHSLGLFSSQL"
     gene            complement(13229..13609)
                     /locus_tag="VIG70_18830"
     CDS             complement(13229..13609)
                     /locus_tag="VIG70_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252652.1"
                     /translation="MSTDFKLTPHADYIHVELAPDYEIMPGGTTQLTLSISDVSARQG
                     QRRVLIEGTIARRSMGTMDSFGFGSLIGSRLTGMSLACCLYGYAPDQQTQFFRDVAEN
                     RGVRIEFFAERDAALRWLGVGQPQ"
     gene            complement(13634..14317)
                     /locus_tag="VIG70_18835"
     CDS             complement(13634..14317)
                     /locus_tag="VIG70_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEY8252653.1"
                     /translation="MWYRRIALALGVLAVVLLAGSGLGVRAGLWPFRVGLGMLAAALV
                     AGLAAAGMSAVALAVPRLRKGSLPVLLFSLLLGVASAAVPLEQVRRAKTLPYINDVTT
                     DTETPPQFSPPQPYEAPFAELQRIAYPELRALELALPPAQSFARARAAAQARGWEIVA
                     ADEAAGRIEAVATTRWFGFKDDIAIRIRAAGGGSRIDMRSRSRVGRSDLGANAKRIQD
                     FLTEVKTGP"
     gene            complement(14330..14863)
                     /locus_tag="VIG70_18840"
     CDS             complement(14330..14863)
                     /locus_tag="VIG70_18840"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY8252654.1"
                     /translation="MLHKIKDLQGDAIHAEDGEVGSVDDLYFDDEDWDVRYLLVNTRR
                     WIAGPKSGPKYLVSPIAIDRERPLDGGDIRVELTREQISRCPTAAEESHLRSSAEVIG
                     CGIEAVDGTIGRVADLVVDDETWEITDVLVDSRTWLPGKLLLISPEVIERIDWPEKKV
                     HLRLAREDILQAPEIRL"
     gene            complement(14899..15351)
                     /locus_tag="VIG70_18845"
     CDS             complement(14899..15351)
                     /locus_tag="VIG70_18845"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEY8252655.1"
                     /translation="MDRKRDRERLERLAWLLDSSIPIPGTRFSIGLEALIGLVPFLGD
                     LAGVLLSSTILAEAARLGASRAVLARMAFNIALEGLVGLIPLAGDVFDAVWKANQRNV
                     RLLGAWLDRPARAERSSKAFLAAMLLGLAAFAFVSMSLTYLLLRWLVA"
     gene            complement(15344..15547)
                     /locus_tag="VIG70_18850"
     CDS             complement(15344..15547)
                     /locus_tag="VIG70_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252656.1"
                     /translation="MRHLGLGLIVAAALAGALGLFIYFTERGVANEIKGFVLLLVAAV
                     LLVGGCVLWELRALRRTLEDKDR"
     gene            15599..16105
                     /locus_tag="VIG70_18855"
     CDS             15599..16105
                     /locus_tag="VIG70_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY8252657.1"
                     /translation="MAIKVGDRLPDGKLMESTEFDSATQCAMAPKEVNVAEAAKGKKI
                     AVFGLPGAYTPTCSAKHVPSYLQNNDQLKKKGVNEIWCVATNDAFVMAHWARDQKALG
                     KIRFLADGSAAWTKALGLELDLNARGMGTRMQRFSMLVDNGVVKQLNVEGPGKFEVSD
                     GGTMLKQL"
     gene            16185..16646
                     /locus_tag="VIG70_18860"
     CDS             16185..16646
                     /locus_tag="VIG70_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252658.1"
                     /translation="MRLSFVIGSALLAASTALYAQGDDKAARRQQLHDAHAKALKACE
                     GKPEAERRACVQQEMCAQAKDPKACQERYAQAAAARAARDKAVKACEGKQGAERGDCM
                     RRETCAQAKDPAQCEARIKEATARRDKIREACKDKTGDEYRACIRAERGKT"
     gene            16712..16990
                     /locus_tag="VIG70_18865"
     CDS             16712..16990
                     /locus_tag="VIG70_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252659.1"
                     /translation="MDDSRGLTITFMDGTKVSFGFPQQGMNAAAKQLKIEEFMKNPFL
                     MVIADGTLMMFPVVNIKSIQMPMAKEDVEQIRLPTHVIRNATLKRGEA"
     gene            17016..19718
                     /locus_tag="VIG70_18870"
     CDS             17016..19718
                     /locus_tag="VIG70_18870"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8252660.1"
                     /translation="MGDLKKLIAGMSLVLFVAGAAAAEPAKKTVSIEGITEWQLANGL
                     KLLTLPDPGADTITVHIVYLVGSRHEGYGEKGMAHLLEHLLFKGSKRHPNLKEEFTRR
                     GGRWNGTTSNDRTNYFETLGATDDNLEWAIAMEADRMVNSFVSKQDLDSEMTVVRNEF
                     EMGENNPGSVLFQRMQQLAFPWHNYGNPIIGQRSDIERVPIDKLQAFYRTWYQPDNAV
                     LIVAGRFEEARAVALVAKHFGPLPRPERVLPAYYTDEPTQDGERRVRLERVGDNHIVM
                     ALYRAPAGSHPDFPAIDVLANILGESPSGRLHRALVQKGIASAAWGAAREMHDPGYLY
                     FGASLGKEDKTAEARDRLVDVVEGLKQDKVRAEELERARTALLNDYEKVQLDTGQLVR
                     SLSEYIAMGDWRLFFLYRDRLRKVTLADVQRVAGQYLKPANRVLGEFVPSERPDRAEI
                     PPTPDLQSALAGYAGGASVSLGEAFDPSPQNIDKRTVKKQLSNGIRVALLPKQTRGGR
                     AIATLTLHWGDEKSLTNRETACNFAGAMLTRGTKKRSRAELKEAFERLNASVSLGGEG
                     ASLEVRGENLIPALRLAAEALREPAFSREEFDEMKRAALTGAEAQRNEPSSLAGVQLA
                     RHLHEYKPGHPHYTPTVEERIALMRAVKLEDAVACYRELLGATGADFAAVGDFDPDAL
                     ARAVDELFGSWKTPRPFARVPSRHFDRPALENELVTPDKANAALRAGLNVRMRDDHAD
                     FPALVLANQLLGGSSTARVPARVREKEGLSYSTYTTFTSSALDDAAAFRVASIFAPQN
                     RARVEQAIREEIGRAVREGFSADEVEAGKRSVLETRRLARSQDRALASRLGHYLFVKR
                     TFAWDSEFEAKIAALTPQQVNAALRRHIDPARLSVVVAGDLKKP"
     gene            complement(19723..20094)
                     /gene="mscL"
                     /locus_tag="VIG70_18875"
     CDS             complement(19723..20094)
                     /gene="mscL"
                     /locus_tag="VIG70_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY8252661.1"
                     /translation="MSFIREFREFAMKGNIVDLAVAVIIGGAFGKIVASLVEDIVMPA
                     IGAIGRVDFKSLAIQVGDAKILYGKFFQTCVDFLIIALAIFVAIKVINRLHRREEAKP
                     APPPRQEQLLEEIRDLLKTQR"
     gene            complement(20091..20633)
                     /gene="greB"
                     /locus_tag="VIG70_18880"
     CDS             complement(20091..20633)
                     /gene="greB"
                     /locus_tag="VIG70_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEY8252662.1"
                     /translation="MSKAFVKEEGDAPEDDEPAAPEPPAHGKNYITPAGYSRLEAELR
                     RLVEVERPEVVKTVAWAASLGDRSENADYIYGKRRLREIDRRVRFLIKRLESAEVVRS
                     AGRDTDQVFFGATVKIRSERETREITIVGVDEVDPANGRVSWVSPIAKALIKAREGDT
                     VTLRSPSGEEPLEILEVSYS"
     gene            20712..21446
                     /locus_tag="VIG70_18885"
     CDS             20712..21446
                     /locus_tag="VIG70_18885"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY8252663.1"
                     /translation="MAENKNEGLDFLTRLRIRELILMGLIAVSAVMANLPQEYVEETL
                     GVSRGTLVACLGIAVIIGLFLYLKTGLFIAVVLLIAGANMPDQIAEGLNISKIPIVLA
                     LVALVGVGLINYVVKLMPTGLEPRPKEKSAEGIRALFYAIEKNNLVYAQKVLSMNFDP
                     NLHHDNGYTPLAYASMRGSLPMIELLLRNGADPIQTTKEGDSPVELALRFGHAEVADG
                     LKVARRAAAEGLPDSEAPAPATVSGS"
     gene            21516..>23751
                     /locus_tag="VIG70_18890"
     CDS             21516..>23751
                     /locus_tag="VIG70_18890"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEY8252664.1"
                     /translation="MELQAPSPTAEQEISRDTLLEKYAKGSEQTVTEVRRRVARALAQ
                     VERENERAQWERRFFQAQEEGFIPAGRINSAAGVALQATLINCFVQPVGDSISELVDG
                     RPGIYTALQEAAETMRRGGGVGYDFSAIRPKGAEVKGTRSRASGPLSYMRVFDRSCET
                     VESAGSRRGAQMGVLRCDHPDIEEFIHAKDQGDLANFNISVGMTDDFMLAVEQNLDVE
                     LVHKAKPSSKDARQREDGLWIYKTLRARELWEQIMRSTYDHAEPGILFLDRINVDNNL
                     RYCETIEATNPCAEQPLPPYGCCCLGSIDLTRFVKEPFSEKTSFDFDRFAKVVGTSIR
                     MLDNVLEVTAWPLEQQRKEAMAKRRVGLGFTGLGDALIMLRLKYDTDDARAMAGRISE
                     SMRDHAYRASAELAKERGAFPLFNADLYLSGTSFATRLPEDLKKLIRENGLRNSHLLS
                     IAPTGTISLAFADNASNGIEPPFSWTYTRKKRVDDGWQEYAVEDHAFRRYRAMGGDMN
                     ALPPYFVTALEISAKSHEKMVATVAPFIDTSISKTVNVPEDYPYAEFQNLYFDAWKSG
                     LKGLATYRPNKILGSVLSVQKTEDFVQDDVNRRIQVKSVPAPVLTSLRWPGRPDLSGG
                     NPAWTYMIEHPQYRFAVFVGHVENGRPHAFEVWVNGSEQPRGLGALAKTLSMDMRASD
                     SAWLKLKLDALAKAGGDEPFEMPFPPRGEKKLMPSVVSAFAQVVRWRVEQLASGLTEE
                     PFQLA"
BASE COUNT         3865 a         8177 c         7945 g         3764 t
ORIGIN      
        1 ctgccgccgg aggagctgat gcgcaagtgg ctgcacgtgc tgcgctccgc gcagcgccac
       61 gcgctgcaga tacccgccgc gaagatgcac gagcgcgcca ccgcgggccg cgaccgcagc
      121 accctcaccc tcgcctacca tgtctaccag atcccggacg ctttcctgca ggcgatccag
      181 gacggcgtgc cggacatggc gaaggtttac gacacgccgc tgccggctgc agtgaagacc
      241 actgcggatg tcattgccta cggttcccgc atggaaaaga aacttgaagc ctggtggaag
      301 gcgctgccgg acaagtcgtg ccagcagaag gtcaagacct actacggcga tcggccgatg
      361 cacgagctgc tcgagcgctg cacctggcac tcggcgcagc acgcgcgcca gatcatcgcg
      421 gtgctcgagc gcctgggggt cgagccgcag gggcgcctcg gcgccgccga ctacgccggc
      481 ctgcccatgc ccaagggcct ctgggagtag tggacaagga aaagtcccgg caggcgctgg
      541 agaaggtttt ccgcgacatg gcgggcgcca tgaccgcggg catggtgtac gtcggggtga
      601 agacggggct ttttcgcctc atggccggca agggcccgct caccgcttcc gacgtcacaa
      661 gaatttccgg attgatgcct cgttatgtcg aagagtggct gaaaggcatg gtggccgccg
      721 gctatctcga cttcgacccc atggcgcaga cctatgcgct tccggaagag cacgcctact
      781 tcctcgcatc ggaggactcg gaccacttcg tcggcgggct gttcgccatg gtgccgccgc
      841 tgatgcgcgt cgcacccgag gtggcgcgcg ccttcactga aggcggcggc gtgcgcttcg
      901 aggacttcgg cgccgactgc gtgagcgcgc tcgacctcat caacagcggc cagtacgaga
      961 gccgcttcgc ggactactgg atgaaatcgc tgcccgaggc cgccgcgcgc cttcacgccg
     1021 gcgggcgcgc gctcgacgtc ggctgcggct cggggcgggt gtgcaccgcg ctcgcaagcg
     1081 cgttccccgc ggccgagatc gtcggcgtcg atccggacga agcttcgatt gaacgagctc
     1141 gaagtttgag cccaaaagct cgcttcgtgg ccggcgacgt ttcctcggtg cagggagcgt
     1201 tcgatttcat cagcctgtgc gacgtgctgc acgacctggc cgagccgctg cagacgctgc
     1261 gccagatccg ggcgctgctc aagcccgacg cgacgctctt catcgtcgag ccgaaggccg
     1321 cagaccggct cgaggacaac cgcaatccgg tggcggcgac cttctacggc ttcagcctgt
     1381 tccactgcat gacgcagtcg ctcgcgcgcg gcgggccggg cctgggcacc tgcctcgggc
     1441 cggcggccac cgaggcgctg gtgcgccagg cgggctacac ccgcttccgg cgcctcgata
     1501 tcaagagcct gaccaacctg ttctactcgg cgcagccatg atcctcaagc tcgccctcgt
     1561 ctggctgatc aacgcggtcg cgctgattgc ggtcgcctac ctgatgccgt cggtcaccgt
     1621 gtcgagcttc ggcgcggcgc tcgccgcggc gctggcgctc ggcctgatca atgccgtggt
     1681 gcggccggtg ctggtgctgc tcacgctgcc ggtcaccctc ctcacgctgg gccttttcat
     1741 cttcgtgctg aacgggctgc tcttctggat ggtcggcgcc tggctgcagg gcttcgaggt
     1801 cggcggattc tggtccggcg tgttcggcgc gatcctcttc agcctcgtgt cctgggcgct
     1861 gtcggcgctg gtgctgaaat gaccttcaag caggctctgc accggcacct gcaggcgctc
     1921 gaccgcatca aagaacacgc tcgcctgttc gccacgccgg tcgagatcca cgagtccgcc
     1981 gacgcggcgc tcgcggttct gcacctgctc tactgcgagg acgcgccggg caagcggccg
     2041 ctgcgcagcc agagccactt gacgctcgtc tatcagcgcc ggggcgccga atgggtcgtg
     2101 gtccaggacc ggcactgccc gatctcctag gaccgaaatc gctgctgcgc cggctcgcgc
     2161 ggcgctggcg cgaccccatc atcatcctgc cgtacctgac gtacggcacg gtgcagaagc
     2221 tcaccgtctg cggccgcgtg ctggaggacg aggggttcct ccccgccgcc gacgccgaca
     2281 cgcgctggcg caacctgatg cgcttctaca agcgcctgga gtccgacgag gtgccggggg
     2341 cgcggctgcg cgcgcgtttt ctcggcgtcg ccaccgaagg ggtgtcggac gacgaggggt
     2401 actttcgcct cgagctggcg ccgcgcggac gcgtcggccc gggtgcctgg caggaagtcg
     2461 agctcgagct gctcgaagca aggtccatca aggcgaccgc gcgggtgctc gtgccctcga
     2521 aaagagcccg cttcggcgtg atcagcgaca tcgacgacac gatcgtgtcg acccacgtcg
     2581 cgaacaagct ccggatgatc ctgaccgtcg cgctgtcgaa cgcgcgcacg cgcaagccgt
     2641 tcctcggcgt ggccgcgttc taccgcgcgc tgcacgccgg cgtgaacccc ttcttctacg
     2701 tctcgaagag cccctggaac ctgtacgcgc cgctggtcga gtacctcgag gcgcagggat
     2761 tgccgctggg cccgctcatg cttcgcgatt tcggtctttc ctcgaacaag gaccacaaga
     2821 gcgaggccat cgtggacatc ctgcgcacct acccgaagct caagttcatc ctgatcggcg
     2881 acagcggcga gcaggatccg gagatctaca cccgcatcgt gcagcgcttc ccggagcgca
     2941 tccgggcgat ctacatccgc tcggtgaatc caaaaagaat tgtcgagata gaaaagctcg
     3001 cttccgaagt tgcccggacc ggctgccagc tggtgctcgc ggcggaagcc gagcccgccg
     3061 cggcgcacgc cgcggccgag ggcctgattc aggcttccga gctgcgcgcg gtgcgcgccg
     3121 agaaggactc ggaaatgagc tcctcgaagc cggcggtgtc gagcggcgcg ctgaagtagt
     3181 gcccctgcca ttcctcgcag ccgagcgcca tgagatgctc gagctgcgcc gcggtctcca
     3241 cgccctcccc ggcgaccgca atgccgagcg tcgaggcgag cgcggtgatt gcgcgcacga
     3301 tcgccgcatc ggccgcctgc gagtcgatcc cgctcaggaa cgagcggtcg atcttcagct
     3361 tgtcgatcgg caggctgcgc agataggcga ggctcgagta gccggtgccg aagtcgtcga
     3421 tggcgaagcg cacgccgagc tcgccgatgc ttttgagcgt ggcgacgttc tcggcgaggc
     3481 ccgacatcag cacgcgctcg gtgacctcga gctccacctg ccggccctcg agctggtgct
     3541 cgcgcagggt tgacttgagc ttctcgacgt aagcggcgcc ctgggagagc tgcggcgccg
     3601 agacgttgac cgcggtccag agcttgtgac cgtagcgccg ctgccacgcg cccgcctgcg
     3661 agaccgcgcg ctcgaggacc cactcgtcga ccgcgcggat caggccgcac tcctcggcga
     3721 gcggcacgaa gtgctccggg gtgagaagcc cgcgggtggg atgctgccag cgcaccagcg
     3781 cctcggcgcc gaccacgcgg ccgcgccagt gcagcgggct cgagcgccgg tcgggcggcc
     3841 agccgcgcac caccggctgc cagtgcagcg cgagctcgcc gcgcgcgagc gccgagcgca
     3901 gctcgttctc caggcgcagg cgctcggcgg cgcgcgcgtt gagcgccggc gcgaacaggc
     3961 gccaggtgcc cttgccgctc tccttggcgt ggtagagcgc ggcgtcggcg ttcttcagga
     4021 gctcgacgaa atcgcggccg tcgttggggt agatgctgat gccgatcgag gcggcgacgc
     4081 tcagcgtctt gctctcgacg gtgaacgctc gcgcgagcag gccgaggatg cgctgcgcga
     4141 gcgccgccgc cgccgcggcc gcgtcctcgc tcggcttgat cgactgcacc aggacgaagt
     4201 cgtcgccgcc gaggcgcgcc agcgtgtcct ccccgtgcac gaggttgccc atgcgctcgg
     4261 ccaccgcgcg cagcagcgcg tcgccggcgc ggtgccccat cgactcgttc accagcttga
     4321 agcggtcgag gtcgatgcac agcagcgcga attgcgagcg gttgcgcgcc gcggtgagga
     4381 tcgcctggct cgcgcggtcg gcgagcagcg tggaattcgg cagcccggtc agggcgtcgc
     4441 gcgtggtgag ctgctcgatg cgcgcctccg cgtgcttgcg cgcggtgacg tcggcgccgg
     4501 tgccgcgcca gccgatcagg cgagcgcggg ggtcgcgcac cggcacgccg ctcaccgatt
     4561 gccagatcac gccgcccgac ttcgtgatca tccggtgcag caggtcgcgg aacggctcgc
     4621 catcggcggc gcggccggcg aaccattcgt ccaccatgcg cggctcgccg agcggcatga
     4681 agtcgcgcgg cttgcgcccg aggagctcgg cgcgcgcata gccgagcacc gcctcggcgc
     4741 gctccgagag gtaggtgtag cggccctggg cgtcggtctc ccagacgtat tcgcccgagg
     4801 cgtcgaccac gtcgcggaag cgctgctcgc gctcggcaag cgccgcctcg gcgcgcttgc
     4861 gctcgtcgat gtcggccacc gtccccgaga cgcgcgtggc gcggccgtgc gagccctgct
     4921 cggtgacgcg cccgcgcgcg tgcagccatt tccacttgcc ctggcgcgtg cggatgcgga
     4981 actcgacgtc gagcgccggc gcttcgccct tcagcgcgcg caccagcgcc tcggtgtagt
     5041 gcacgcggtc gtcgggatgc atcatcgcga tcagttcggc gccgagcgtc accgtgggcg
     5101 acgcggtgtg gccgaggaag cgcacccagc cgtcgccggc ggagagctcg ccgcgctcgg
     5161 cgtcgaagtg gaactcggcg aggttgccgc cgtcgagcgc gagctcgagc cgctccttcg
     5221 cgcggaacag cgcccgctcg gcgctgcgct gctcggtgac gtcgcgcgcc acgccgcggt
     5281 aaccgaggaa agccctgtta tcagcggcca ggcgcgactg gcccgagatg atgtgcacct
     5341 ggcgcgcgcc gcgctcgtcc acgcgcgcga tttccaggtc gaagaacgat tgccgggccg
     5401 cgagctgctc gaggtgcgcc tccagcgcgc gcggctcgac ctcgaccccc gggatctccc
     5461 agatgcgctt gccgaaggtc ggcgtctggc cgaagaacgt cgccaccggc gcgccgcccg
     5521 agagccagac gatgcggtgt tcggcgtcgg tctcccagaa ccagtcggca gacaactcgg
     5581 tcaggccgcg gaagcgggcg gagtctgctt ttgcatccag caatgctttc tctcccctgc
     5641 gggaaagcac gtgcatcgcc agatagacgg cgccgcccga agccgcgacc aggagcagga
     5701 tttcgaacat gcgggcctag cccgccgccg cgtcgcgcac cggcggcgcg accttcagga
     5761 cttcgtcgat cgtggtggcg ccggcgccca ccttcaccgc gccggagatg cgcagcggct
     5821 tcatgccttc cttgaaggct tgctcgcgca gggccacgag gtccgcgttc tgggacacca
     5881 ggcccttcag ggcctgggaa aacaccagcg tctcgtagat cccgacccgc cccatgtagc
     5941 ccgtcatgcg gcattcgagg cagccgaccg gcttgtagag gcgcgccgga tgcttggatt
     6001 tccacggcgc gaccagcgcg ttccaggcgt cctggtcctc cgcgcggttg agctccaccg
     6061 tctgcttgca atgcgggcag agcgtgcgca ccaggcgctg cgccatgacg ccattgagcg
     6121 tggcgttcag caggtacggc gccacgccga gctcgatcag gcgggcgatc gcgctcggcg
     6181 cgtcgttggt gtgcagggtg gacagcacga ggtggccggt gagcgccgac tgcaccgcca
     6241 tttccgcggt ttccaggtcg cggatctcgc ccaccatgat gatgtccggg tcctggcgca
     6301 tcagcgcgcg cacgccctcg gcgaagccga gctcgatcgc gctcaccacc tgcatctggt
     6361 tgaacgccgg ctcgaccatc tcgatcgggt cctcgatggt gcacacgttc acttcggagg
     6421 tcgcaaggct cttcagcgtg gaatacaagg tggtcgtctt gcccgagccg gtcggcccgg
     6481 tcaccagcac gatgccgttc ggccggtcgg tcatctcctt ccagcgcttc tggtcctcgg
     6541 ccgagaagcc gagctcgctg aaatcgcgca ccagcacctc ggggtcgaag atgcgcatca
     6601 ccagcttctc gccgaacgcg gtcggcaggg tcgagatgcg cagctcgatg tcctgctcgg
     6661 cgctgcgcgt cttgatgcgc ccgtcctgcg gccggcgctt ctcgaccacg tccatgcgcg
     6721 ccaggatctt gatgcggctg gtcatcgcgg cgagcaccga ggccgggatc tggtagacct
     6781 ggtgcagcac gccgtcgatg cggaagcgca cgatgcccag gtcgcgccgc ggctcgacgt
     6841 ggatgtcgct cgcgcgctgc tcgaaggcgt actgccacag ccagtcgacc acgcgcacca
     6901 cgtgcgcgtc gttggcgtcg agcttgtcgc tcttgccgag ctcgaccagc tgctcgaagt
     6961 tcgacaggcc gctgatcgag gtgttcttgt tggcgccctt gatcgatttc gcgaggttgt
     7021 agaactcgac ctggtagcgc tcgatgtcga gcgggttcgc gatcacgcgc cggatctcgc
     7081 gcttgatcat gcgtgcgagc tcgggctccc agtcgcggat tcccggctcc gcggtggcga
     7141 tcaccgcttc cttcgagttg agcgccaccg gcaggatgcg gaagcgcgtg gcgtacgccg
     7201 acgacatgat ctcggtcacg ccggtgaagt cgatcttgag cggatcgatg tgcaggtact
     7261 ccaggctcga gcgcttcgcc agccactcgg tgagcgcgtc gagcgtgaga agagcgctgc
     7321 ccggggcggt tgctcccttc ttccacttct gatcggcgac gatcaccagc ggatgcatcg
     7381 tgccgcggta gtagcgccgc tccttcttca gcttctccgc cgcctcgggc tcggccatct
     7441 tgtccgcgac cagccagtcg agcacctcgg caagcgtcag ccgagccccg ctctgcgtct
     7501 tcccctccgg ggtcttaact acttgttgca taggcgaaag actataccgc aaggcgcttc
     7561 gcccatcagg gaggaagtcc tcctccacac acccccttag aatcgcctgc gtgcgaaccg
     7621 cagccaagcg cgccctcgcg ccggacgtca aattgcgcga ggtctgggcc tggagcatgt
     7681 acgacttcgc caattcggcg tacaccacgg tcgtcatcac cgccgtcttc agcgcgtact
     7741 tcgtcggcgt cattgcccag aatgctccct ggtcgacgtt cgcctggacg gctgccctct
     7801 cggtgtcgta cgccgcgatc ctcctgaccg gtccgctcgt cggcgcctgg gccgacgcgc
     7861 acgcggcgaa gaagccgctg ctgctcgcga ccacgctggg ctgcgtagtg ttcacggccc
     7921 tgctgtattt cacgcagccg ggcgccgtcg ccgtcgcgct gctcctcctc atcgcgtcca
     7981 attatttctt cggcgcgggc gagaacctga tcgccgcgtt cctgcccgag ctcgccgatt
     8041 cgcgcgcgat ggggcgcgtg tcgggctggg gctggagctt cggctacctc ggcggcctga
     8101 cggcattggg aatctgcctc ttttacatca cttcttccga aggcaagcct gcttcgcaaa
     8161 cggtcccggt cacgatgctg atcaccgcgg cgttcttcgc gctcgccgcg gtgccgacgt
     8221 tcgtcttcct gcacgagcgc gcagtgccgc aggcgcgcat ggagaacccg tgggcacggg
     8281 tcaggcagac actgcgcgag accgagcgct acgcggatct caggcggttc ctggtctgcc
     8341 tgcttttcta ccaggcgggg atcaccgcgg tcgtctccct cgccgcgatc tacgccgagc
     8401 aggcgatgaa gttcaccacg cagcagacca tcgcgctgat cctggtggtc aacgtgaccg
     8461 cggcgatcgg cgcgttcggc ttcggctacc tgcaggacgc cgtcggccac gttcgcgccg
     8521 tctcgctcac gctcgtcgga tggatcgtga tggtggtgct cgccggcctg tccgaatcga
     8581 gcgcttcgtt ctgggtggcg gcgaatctcg ccggcctgtg catgggctcg tcgcaggccg
     8641 ccggccgcgc ggtcgtgggc tacctcgcgc cgccggcgcg gctcgccgag ttcttcggcc
     8701 tgtggggcct cgcggtcaag gcggcgtcga tcttcggccc cctcacctac ggcgcggtga
     8761 ccttgatctt cgccggcaac caccgcctcg gcatcttcgt caccggcatc tatttcgtga
     8821 tcggcctcat cctgctgcgc aatatcgacg tcgagcgagg gcgcgccgcg gcgctcgcgg
     8881 catgagacat ctcttgatga ggctgtttct ggccatgctg ctcgccgcgg ccgcggccgg
     8941 aacgctcgcc gggccgcggg gcgacaccag cgagtggagc ctcaacctgt tcgtcgtcgg
     9001 ctcgaaccac tacgcgttcg aaggcggcgc gagcgcgcgc agcgacaccg gcgcgggctt
     9061 cggcttctcg gtggcgcgca acctgaacga ctacttcgcg gtgggcgtgg aaggctcgct
     9121 cgccacgttc agctaccgcg cgagcatcgc accgggcagc ggcaacggcg gcgccggctt
     9181 cgagacccgc ggcgacatgg aaagcgcgat gctgcgcgtg catgcaacct ggaacctgct
     9241 cgcgcggccg ctcacgccct tcgtcaccgc cgccgccggc gtgattttcc tcgagcccga
     9301 tttcggcgcc cagccgccgg cggacgcctg ctgggcctat ccgtggtacg gcgaggtctg
     9361 cggcgcgcgg gcacccacca gcacgctcgc gcgcttgagc tacggcgccg gcgccggcct
     9421 gcgcttcgat ttcccccgcg aacggggctt catccgcgcc ttcgtcggcg gcgaatggat
     9481 cgagatgtcg gaagcgccga gcacggtcgg ctacgtcacg gtgcgcgccg acttcggact
     9541 gcgcttctag gacacccgct caagtcccgg gcgcgaagct tgcagaaagg ggttcaccat
     9601 gagaaccctc gctcctcatt tcctctccct ggtccttttg actgccttcg ccaccgtggc
     9661 cccggcgcag gccccggaaa aacaggcggc gaagtccgcc gccggcgggc gcaccgccaa
     9721 cctccccgac ttcactgcgc tgatgaagca gcatgggccc gcggtggtga acgtcatcac
     9781 caagcgcgag cccgcccgga ccgcgcgcgc tccccaggaa aagcaggcgc ccgacgaccc
     9841 gctgcaggag ttcttccgcc gcttcatgcc cgattcgccg gaacgcgcgc ccggccccgg
     9901 ccaggggctc ggctcgggct tcatcatcag ccaggacggc tacgtgctta ccaacgccca
     9961 cgtggtcgcg ggcgacggcg aagtcacggt gcggctggcc gatgccaagc gcgagttcaa
    10021 ggcgaaggtg atcggcgcgg acgagcgcac cgacgtggcg ctcctcaaga tcgacgccac
    10081 cgggctgccc acggtgaagc tcggaaaatc ggcatcgctc cagcccggcg aatgggtcgc
    10141 ggcgatcggc tcgccgttcg gcttcgagaa cacgatcacc gccgggatcg tgagcgcgac
    10201 cgggcgctcg ctgcccgcgg agacctacgt gcccttcatc cagaccgacg tcgcggtgaa
    10261 ccccgggaat tcgggcgggc cgctcatcaa cctcgcgggc gaagtggtcg gggtgaactc
    10321 catgatctac agccagaccg gcggctacat gggcgtgtcg ttcgcgatcc cgatcgaggt
    10381 cgcgctcgac gtgagcaggc agctccgcgc cgagggcaag gtgacccgcg gccggctcgg
    10441 cgtgcgcatc cagccgatga cgaaggagct ggcgcagtcc ttcaagctga acgagccgaa
    10501 cggcgcgctc atcgccacgg tcgagcccgg cagccccgcg gacaaggcgg gactcaagcc
    10561 cggcgacgtg gtgctcgcct acaacggcca ggagatcgac gacccgaaca agctgccgcg
    10621 cctcgtggcc gcgaccaagc ccgggcagag cgccacgctg aaaatctggc gcggcggcaa
    10681 ggccgaggaa gtgaagttca ccgcggccga gctggtcgcc gataccaggg ccgcgaagcc
    10741 cgcccaggac aaggcgatca agccgaaccg cctcggcctg gtggtgagcg agctgccgcc
    10801 ggcgcagcgc cgggcgctcg gcatcgagta cggcctggtg gtcgagagcg ccgaggccgg
    10861 gcgcacgccg cttcgccccg gcgacgtcat cgtcggcgtc gggcgcgaga gcgtgaagag
    10921 cgtcgaggac ttcgaccggt tgatcggcga gcagaagcag ggcgacaccg tcgcgctcct
    10981 cgtgcgccgc ggcgaagcga ccgtctacgt cccggtcgag gtcggatagc gaattcgggg
    11041 tcagggtgcg aattcggtta ttgcgtacgc aaaaacgagt tcgcaccctg accccgaatt
    11101 ccttcctgta gaagttatat gacacagaag ctcttacttg ggcgctgatt gagccgtagg
    11161 gagtaagcgg cgctctgcgc ccgggcgcgc cgcttgcagc agttggagcg gactacatgc
    11221 catgcgcagc ctcgtgttgc tggttctggt tttcctctcc tcgtgtaccc cggcgccgga
    11281 gggactgagg ctcgtgacca tcgacaaccc cgagcggggc ctgagcctgc gcgagttgcc
    11341 gcccgaagcg ctgaagtcgc tcgggctgcc ttacggcctc gcggtggtga gggcgggctc
    11401 gctcgcccag cgcgccggcc tgaagatcgg cgacgtcgtc tacggcgtga accagagaaa
    11461 gctgaagaac ctcgaggagc tcaaccaggt gctggccgag caggagcatg gaaagctggg
    11521 gctgctcgtg cgccgggggc gctcggactt ctacgtcgcg gtggacctcg gcggcgcgcc
    11581 aggcgtgccg agaggcctgc ccgcgcctag agatactctt ctgcgcactt gatgatttcg
    11641 gtgttcgagg gcaggtcgag gcagtgacgc gcgtcctcgt gccagcggcg cgcgtgcggc
    11701 attcgcgggc cggtcgctgc ctgcctggcc tccggcttcg tcgcttcttt catggcttct
    11761 gccttggcgg gttccttggc ggcgggcttt tcctgcgcca aggccgtcgc ggccaaagaa
    11821 acgagtgcca aaaaaatcat atgtttcatg ggactacctt gttttcatga aacgatacct
    11881 gaagaatacg ccatttttgc taaagctgcg aactgaaaag tcccagactg tgctcccaaa
    11941 gcttctcggt ccaggggcgg ttttcccagt cttcgagcga gacgcgcttc gagcgccgta
    12001 ggtcggcctc gaagatgcgg gcctgggccg cggcgaagtc gcggtcgtag acgttcaagt
    12061 tcgcctcgtc gttgacgctg aaggagcggg cgtcgaagtt ggtcgagccg accgagaccc
    12121 acaggtcgtc gaccaccatc accttgcagt gaaagaacgt cggctggtat tcgtagaact
    12181 cgatcccggc gcgcagcagc tcgccccagg tcgagcgcga agcgcggcgc accaggggcg
    12241 agtcggtatg cggcccggga acgatcagct gcacgcgaac gccccgcttc gccgccgcga
    12301 ccagggtgcg cacctcgacc ccgtcgggca cgaagtacgc ggccgagagc cggatcgact
    12361 tcgccgccgc cgcgatcgac atgagataca tgagctgcat gctctccgag ccgccgccgg
    12421 gcgaggtgac gatgaactgc gccgcctggg tgccggcgcg ggggagcgcc ggaaagtatt
    12481 cctcgccgtg cagcacgcgg ccggtgacct cgatccagtt ttcgatgaag gccgcctgca
    12541 cctgcgccac caccgggccc tcgatgcgga agtgggtgtc gcgccagtgc ttcgggtcct
    12601 gcgcgtcgcc gcgccactcg tcgccgatgc cggcgccgcc ggtgtagccg atgcgcccgt
    12661 ccaccaccat gatcttgcgg tgggtgcggt tgttgatgcg cgcgagcgtg ttccagcgcc
    12721 acgggttgta cttctccacc tcgatgcccg ccgccttcat cgccttcacc gatttgtcgt
    12781 cgatcttgcc gctgccgagc gcatcgaaca gcaggtgcac cttgacgccg gcctgcgcgc
    12841 gctcgatcag cgcctgcgtg aactcggccc cggtctcgcc cgaccagtag atgtagctct
    12901 cgaacgtgat ggtcttctgc gcgctgcgga tcgccttcag catgtcggcg aagaaccggt
    12961 cgccgttgac cagcgcctgc gcgcggttgc ctccgacgag cgccggcccg aacaccacgt
    13021 tcatgttgcg cgcgaactgc tcgtcggcgg tggaatagag cgtcgcgacg cgctgctcga
    13081 tgtgcttgtc gcccaggctg aggttcgcga ccagcatcac ggcgagcgcg gtcgccgcga
    13141 aggtgaaccc ggcgatcagc cacttgttgg agctggaatg gagcttggcc cggcgcatgc
    13201 cgccgagcct cgcacgacgg cattacagct attggggctg ccctaccccc aaccagcgca
    13261 gcgcggcgtc gcgctcggcg aagaactcga tgcgcacgcc ccgattctcg gccacgtcgc
    13321 ggaagaactg ggtctgctgg tcgggcgcgt agccgtagag gcagcaggcg agcgacatcc
    13381 cggtgagccg gctgccgatg agcgatccga agccgaacga atccatcgtc cccatgctgc
    13441 ggcgcgcgat cgtgccttcg atcagcacgc ggcgctgccc ctgccgcgcg ctcacgtccg
    13501 agatcgagag cgtgagctgc gtagtgccgc cgggcatgat ctcgtagtcg ggcgcgagct
    13561 cgacgtggat gtaatcagcg tgcggcgtca gcttgaaatc ggtgctcatg cgcgcggggc
    13621 gcatgatagc gggctacggc ccggtcttca cttctgtcag gaaatcttga atgcgtttcg
    13681 cgttcgcgcc caggtcgctg cggccgacgc gcgacctgga gcgcatgtcg atgcggctgc
    13741 cgccgccggc ggcccggatg cggatcgcga tgtcgtcctt gaagccgaac cagcgtgtgg
    13801 tcgcgaccgc ttcgatgcgc cccgccgcct cgtccgcggc gacgatctcc cagccgcgcg
    13861 cctgcgcggc cgcccgcgcg cgcgcgaagg actgcgccgg cggcagcgca agctccagcg
    13921 cccgcagctc gggataggcg atgcgctgca gctcggcgaa cggcgcttca tacggctgcg
    13981 gcggcgagaa ctgcggcggc gtttcggtat cggtcgtgac gtcgttgatg tacggcagcg
    14041 tcttcgcgcg gcgcacttgc tcgagcggca ccgccgcgct cgcgactccc agcaagagac
    14101 tgaaaaggag gacaggaaga gaacccttgc gcagacgagg aacggcgagc gccacagcgg
    14161 acatcccggc tgccgcaagc ccggcgacga gggcggcggc gagcatgccg agcccgaccc
    14221 ggaacggcca cagcccggcg cgcacgccaa gacccgagcc ggcaagcagc acgaccgcga
    14281 ggacgccgag cgcaagcgcg atgcgcctgt accacatcga ttcagactac tacagccgga
    14341 tttcgggggc ctgcaggatg tcttcgcgcg cgaggcgcag gtgcaccttc ttctccggcc
    14401 agtcgatgcg ctcgatcacc tcgggcgaga tgagcagcag cttgcccggc agccacgtgc
    14461 gcgaatcgac cagcacgtcg gtgatctccc aggtctcgtc gtcgaccacc aggtcggcga
    14521 cgcggccgat cgtgccgtcc accgcttcga tgccgcaacc gatgacttcg gcagaggagc
    14581 gcaggtgcga ttcctcggcg gccgtcgggc accggctgat ctgctcgcgc gtgagctcga
    14641 cacggatgtc gccgccgtcc agcggacgct cgcggtcgat cgcgatcggc gagaccagat
    14701 acttggggcc ggacttcggc ccggcaatcc agcgccgcgt attgaccagc aggtagcgca
    14761 cgtcccagtc ctcgtcgtcg aagtagaggt cgtcgaccga cccgacctcg ccgtcctcgg
    14821 cgtggatcgc gtcgccctgc aggtccttga tcttgtgcag catcctaact ctcctgagca
    14881 agcgctgcgc ctgcgtggct aggccacgag ccagcgtagc aataaatacg tcagggacat
    14941 cgaaacgaag gcgaacgcgg ccaggcccag gagcatggcg gccaggaaag ccttgctgga
    15001 gcgctccgcc cgcgccggac gatcgagcca tgcgcccagc agccgcacgt tgcgctggtt
    15061 cgccttccac acggcgtcga acacgtcgcc ggcgagcggg atcaggccga ccagtccctc
    15121 gagcgcgatg ttgaacgcca tgcgcgcgag caccgcgcgg ctcgcgccaa ggcgcgcggc
    15181 ctcggccagg atcgtgctgg acaggagcac cccggcgaga tcgcctagga acggcaccag
    15241 gccgatgaga gcctcgagcc cgatcgagaa gcgcgtgccc gggatcggga tcgagctgtc
    15301 gaggagccac gccaggcgct cgaggcgctc gcggtctcgc ttcctatcga tccttgtctt
    15361 ccagggtacg gcgcagcgcg cgcagctccc agagcacgca gccgccgacg agcagcaccg
    15421 cggcgaccag cagcaggacg aagcccttga tctcgttcgc cacgccgcgc tcggtgaaat
    15481 agatgaaaag gccgagcgcg ccggcgagcg cagccgccac gatcaggccg aggccgagat
    15541 gtcgcatatc cccttgctaa aggtatcatt tcgctccccc catccaagga gaatccccat
    15601 ggcgatcaag gtcggcgacc ggctgcccga tggaaagctg atggaatcca ccgagttcga
    15661 ctcggcgacg cagtgcgcaa tggcgccgaa ggaagtgaac gtggccgagg cggcgaaagg
    15721 caagaagatc gcggtcttcg gcctgccggg cgcgtacacc ccgacctgct cggcgaagca
    15781 cgtgccgagc tacctgcaga acaacgacca gctcaagaag aagggcgtga acgagatctg
    15841 gtgcgtggcg acgaacgatg cgttcgtcat ggcgcactgg gcgcgcgacc agaaggcgct
    15901 gggcaagatc cgcttcctgg ccgacggcag cgccgcctgg accaaggcac tcggcctcga
    15961 gctcgacctc aacgcccgcg gcatgggcac gcgcatgcag cgcttctcga tgctggtgga
    16021 caacggcgtg gtgaagcagc tcaacgtcga gggcccgggc aagttcgagg tctccgacgg
    16081 cggcacgatg ctcaagcagc tctgaaatat atcgttacaa atgcctgtcg gcggcgcagc
    16141 gtggcgcgcg tttcttggga tgggaattcc cccaaggaga gctcatgaga ctttcattcg
    16201 taatcggtag cgcgctgctc gctgcgagca ccgccctcta cgcgcaaggc gatgacaagg
    16261 cggcgcgccg ccagcagctg cacgacgcgc acgcgaaggc gctgaaagcc tgcgaaggca
    16321 agcccgaagc cgagcgccgc gcctgcgtgc agcaggagat gtgcgcccag gcgaaggacc
    16381 ccaaggcctg ccaggagcgc tacgcccagg ccgccgcggc cagggccgcg cgcgacaagg
    16441 ccgtcaaggc ctgcgagggc aagcagggcg ccgagcgcgg cgactgcatg cgccgcgaga
    16501 cctgcgcgca ggccaaggac ccggcgcagt gcgaggcgcg catcaaggaa gcgaccgcga
    16561 ggcgcgacaa gatccgcgag gcctgcaagg acaagacagg cgacgagtac cgggcctgca
    16621 tccgggccga gcgcggcaag acatagtcca accgctacgt ccagccgcta agccgcgccg
    16681 ctacaatgga ccctcccgca aggagggtcc gatggacgat agccgtgggc tgacgatcac
    16741 tttcatggac ggcacgaagg tttcgttcgg ctttccgcag cagggcatga acgccgccgc
    16801 caagcagctc aagatcgagg agttcatgaa gaaccccttc ctgatggtga tcgcggacgg
    16861 gaccctgatg atgttcccgg tggtgaacat caagtcgatc cagatgccca tggccaagga
    16921 agacgtcgag cagatccggc tcccgaccca cgtcatccgc aacgcgacgc tcaagcgcgg
    16981 cgaagcctag ggctcctggc atcgcatttt tttgcgtggg tgatctgaaa aaactcattg
    17041 cagggatgtc gctggtcttg ttcgtggcag gcgcggctgc ggccgagcct gcgaagaaga
    17101 ccgtctccat cgaaggcatc accgagtggc agctcgccaa cggcctgaag ctcctcaccc
    17161 tgcccgaccc cggcgccgac acgatcacgg tgcacatcgt gtacctcgtc ggctcgcgcc
    17221 acgagggcta cggcgagaaa ggcatggcgc acctgctgga gcatctcctg ttcaaaggct
    17281 ccaagcgcca cccgaacctg aaggaggagt tcacgcgccg cggcgggcgc tggaacggca
    17341 ctacctcgaa cgaccgcacc aactacttcg agaccctcgg cgccaccgac gacaacctcg
    17401 agtgggcgat cgcgatggag gccgatcgca tggtcaactc cttcgtgtcc aagcaggacc
    17461 tggacagcga gatgaccgtg gtgcgcaacg agttcgagat gggcgagaac aaccccggct
    17521 cggtgctgtt ccagcgcatg cagcagctcg ccttcccgtg gcacaactac ggcaacccga
    17581 tcatcgggca gcgctccgac atcgagcgcg ttccgatcga caagctgcag gccttctacc
    17641 gcacctggta tcagccggac aacgcggtgc tgatcgtcgc cggccgcttc gaggaggcgc
    17701 gcgcggtcgc cctggtcgcc aagcacttcg ggccgcttcc gcgccccgag agagtcctgc
    17761 ctgcctacta caccgacgag ccgacgcagg acggcgagcg ccgcgtgcgc ctggagcgcg
    17821 tcggcgacaa ccacatcgtc atggcgctgt accgcgcacc ggcgggcagc caccccgact
    17881 tcccggcgat cgacgtgctg gcgaacatcc tgggcgaatc gccgagcggc cggctgcacc
    17941 gcgcgctcgt gcagaaggga atcgcgtccg ccgcctgggg cgcggcgcgc gagatgcacg
    18001 atcccggcta cctgtacttc ggcgcctcgc tcggcaagga agacaagacc gccgaggccc
    18061 gcgaccggct ggtcgacgtc gtcgagggct tgaagcagga caaggtgcgc gccgaagagc
    18121 tcgagcgcgc gcgcacggcg ctcctcaacg actacgagaa ggtgcagctc gacaccggcc
    18181 agctggtgcg ctcgctctcc gagtacatcg cgatgggcga ctggcggctc ttcttcctct
    18241 accgcgaccg gctgcgcaag gtgacgctcg ccgacgtgca gcgcgtggcc gggcagtacc
    18301 tcaagcccgc caaccgcgtg ctgggcgagt tcgtcccgag cgagcgtccc gaccgcgccg
    18361 agatcccgcc gacgcccgac ctgcagtcgg cgctcgcggg ttacgccggc ggcgccagcg
    18421 tgagcctcgg ggaagccttc gatccgtccc cgcagaacat cgacaagcga accgtcaaga
    18481 agcagttgtc gaatggaatc cgggtcgcgc tccttcccaa gcagacccgc ggcgggcggg
    18541 cgatcgcgac gctgacgctg cactggggag acgagaagag cctcacgaac cgggagacgg
    18601 cgtgcaactt cgccggggcg atgctcacgc gcggcacgaa gaagcgcagc cgcgccgagc
    18661 tgaaggaggc cttcgaacgg ctgaatgcca gcgtctcgct gggcggcgaa ggcgcgagcc
    18721 tcgaggtgcg cggcgagaac ctgattcccg cgctgcgcct cgcggccgag gcgctgcgcg
    18781 agcccgcgtt ctcgcgcgag gagttcgacg agatgaagcg cgcggcgctc accggagccg
    18841 aggcgcagcg caacgagcca tcgtctcttg ccggggtgca gctcgcgcgc cacctgcacg
    18901 aatacaagcc cggccacccg cactacaccc cgaccgtcga ggagcgcatc gccctgatgc
    18961 gcgccgtgaa gctcgaggac gcggtcgcgt gctaccgcga gctcctcggc gccaccggcg
    19021 ccgacttcgc cgcggtcggc gacttcgacc ccgacgcgct cgcgcgcgcg gtggacgagc
    19081 tgttcggctc gtggaagacg ccgcgcccgt tcgcgcgcgt gccttcgcgc cacttcgatc
    19141 gcccggcgct ggagaacgag ctggtgaccc ccgacaaggc gaacgcggcg ctgcgcgccg
    19201 ggctcaacgt gcgcatgcgc gacgaccacg cagatttccc ggcgctggtg ctggcgaacc
    19261 agctgctcgg cggcagctcg accgcgcggg tgccggctcg cgtgcgcgag aaggaaggcc
    19321 tctcctacag cacctacacc acgttcacgt cgagcgcgct cgacgacgcc gccgcgttcc
    19381 gcgtcgcctc gatcttcgcg ccgcagaacc gcgcgcgcgt cgagcaggcg atccgcgagg
    19441 agatcggccg cgcggtgcgc gagggcttca gcgccgacga ggtcgaggcc ggcaagcgct
    19501 cggtgctcga gacgcggcgc ctcgcgcgct cgcaggaccg cgcgctcgcg agccggctcg
    19561 gccactacct cttcgtcaag cgcaccttcg cctgggacag cgagttcgag gcgaagatcg
    19621 ccgcgcttac gccgcagcag gtcaacgccg ccctgcgccg gcacatcgac ccggcgcggc
    19681 tgtccgtcgt cgttgccggg gatctaaaga aaccctgaaa acttagcgct gggtcttcag
    19741 caagtcgcgg atctcctcga gcagctgctc ctgccgcggc ggcggcgcgg gcttcgcttc
    19801 ttccctgcga tgcaggcggt tgatcacctt gatggcgacg aagatcgcca gcgcgatgat
    19861 caggaagtcg acgcaggtct ggaagaactt gccgtagagg atcttcgcgt cgccgacctg
    19921 gatggcgagg ctcttgaagt cgacgcgccc gatggcgccg atcgccggca tcacgatgtc
    19981 ctcgaccagc gaggccacga tcttgccgaa ggcccccccg atgatcaccg cgaccgccag
    20041 gtcgacgatg ttccctttca ttgcgaactc gcggaactcc ctgatgaagc tcatgagtag
    20101 ctcacctcca gtatttcgag cggttcctcg ccggatgggc tgcgcagggt gaccgtgtcg
    20161 ccctcgcgtg ccttgatcag cgctttcgcg atcggcgaga cccacgacac gcgcccgttc
    20221 gcgggatcca cttcgtcgac gccgacgatg gtgatctcgc gggtttccct ttccgaccgg
    20281 atcttcactg tcgcgccgaa aaatacctga tcggtgtcgc ggccggccga gcgcaccacc
    20341 tcagcgctct cgagccgctt gatgaggaag cgcacgcgcc ggtcgatctc gcgcagtcgc
    20401 cgcttgccgt agatgtagtc ggcgttctcg gagcggtcgc cgagcgacgc ggcccaagcc
    20461 accgtcttca ccacttccgg gcgctcgact tcgacgaggc ggcgcagctc ggcttcgagc
    20521 cgcgaataac cggcgggcgt gatgtaattt tttccatgcg ccggtggctc cggcgccgca
    20581 ggctcgtcgt cttcgggagc gtcgccttct tccttgacaa aggccttgga catcaaaggc
    20641 attctatcgc ttgcaagggc ggcggcgacc tttcagaatg cgctcgggga tgggggatag
    20701 gggaaaggga gatggcggag aataagaacg aagggctgga tttcctgacg cggctgcgca
    20761 tccgcgagct gatcctgatg gggttgatcg cggtcagcgc ggtgatggcc aatctgccgc
    20821 aggagtacgt cgaggaaacg ctgggggtca gccgcggcac gctggtcgcc tgcctgggca
    20881 tcgcggtcat catcgggctc ttcctgtacc tcaagaccgg actcttcatc gccgtggtgc
    20941 tgctcatcgc cggcgccaac atgccggacc agatcgccga aggcctgaac atctcgaaga
    21001 tcccgatcgt gctcgcgctg gtggcgctgg tcggcgtcgg cctgatcaac tacgtcgtga
    21061 agctgatgcc gaccggcctc gagccccggc cgaaagagaa gagcgcggaa gggatccgcg
    21121 cgctgttcta cgcgatcgag aagaacaacc tcgtctacgc gcagaaagtg ctgtcgatga
    21181 acttcgatcc caacctgcac cacgacaacg gctacacccc cctcgcctac gcctcgatgc
    21241 gcggcagcct gccgatgatc gagctcctgc tgcgcaacgg cgccgatccg atccagacca
    21301 ccaaggaagg cgacagcccg gtcgagctcg cgctgcgctt cgggcacgcc gaggtcgccg
    21361 acgggctcaa ggtggcgcgc cgcgccgccg ccgaggggct gcccgacagc gaagctcccg
    21421 cgcccgcgac cgtttcgggc agctagaatt tcccgtttcg aggaggccgc gccgggattt
    21481 tgccgctggg gccaccactg tataatcgtc cagtaatgga actccaggcg ccctccccga
    21541 ctgccgagca ggaaatcagc cgcgacacgc tgctggaaaa gtacgccaag ggcagcgagc
    21601 agacggtcac ggaagtccgg cgccgggtgg cgcgcgccct cgcccaggtc gagcgcgaga
    21661 acgagcgcgc gcaatgggag cggcgcttct tccaggctca ggaagaaggc ttcatccccg
    21721 cgggccgcat caattccgcc gccggggtcg cgctgcaggc caccctgatc aactgtttcg
    21781 tgcagccggt cggcgactcg atttccgagc tggtcgacgg gcggccgggc atttataccg
    21841 cgctccagga agccgccgag accatgcggc gcggcggcgg cgtcggctac gacttctccg
    21901 cgatccgccc caagggcgcc gaagtcaagg gcacgcgttc gcgcgcaagc ggcccgcttt
    21961 cctacatgcg cgtgttcgac cgctcgtgcg agaccgtcga gtcggcgggc tcgcgccgcg
    22021 gcgcgcagat gggcgtgctg cgctgcgatc acccggacat cgaggaattc atccacgcca
    22081 aggaccaggg cgacctcgca aacttcaaca tctcagtcgg catgaccgac gatttcatgc
    22141 tggcggtcga gcagaacctg gacgtcgagc tggtgcacaa ggcaaagcct tcgtcgaagg
    22201 acgcgcgcca acgcgaggac ggcctgtgga tctacaagac cctgcgcgcg cgcgagctgt
    22261 gggaacagat catgcgctcg acctacgacc acgccgagcc cgggatcctg ttcctcgacc
    22321 gcatcaacgt cgacaacaac ctgcgctatt gcgaaaccat cgaggccacc aacccatgcg
    22381 ccgagcagcc gctgcctcct tacggttgct gctgtttggg ttccatcgac ctcacgcgat
    22441 ttgtcaagga acctttttcc gaaaagacct ccttcgactt cgatcgtttc gcaaaggtcg
    22501 tgggcacgtc gatccgcatg ctcgacaacg tgctggaagt gaccgcgtgg ccgctcgagc
    22561 agcagcgcaa ggaagcgatg gcgaagcgcc gcgtcggcct cggatttacc gggctgggcg
    22621 acgcgctcat catgctgcgc ctgaagtacg acaccgacga cgctcgcgcc atggcggggc
    22681 gcatctcgga aagcatgcgc gaccacgcgt accgcgcctc cgccgagctc gcgaaggagc
    22741 gcggcgcgtt cccgctgttc aacgcggatc tctatctatc cggcacgagc tttgccacgc
    22801 gccttcccga agacctgaaa aagctgatca gggaaaacgg gctgcgcaac tcgcacctgc
    22861 tgtcgatcgc gccgaccggc accatcagcc tcgcgttcgc cgacaacgct tcgaacggca
    22921 tcgagccgcc cttctcctgg acctatacgc gcaagaagcg cgtcgacgac ggctggcagg
    22981 agtacgcggt cgaggaccac gcgttccgcc gctaccgggc catggggggc gacatgaatg
    23041 ccctgcctcc ctacttcgtt accgcgctcg agatcagcgc caagtcgcac gagaagatgg
    23101 tggcgacggt tgcgccgttc atcgacacca gcatctccaa gacggtgaac gtcccggagg
    23161 actatcccta cgccgagttc cagaacctct acttcgatgc ctggaagtcg ggactcaagg
    23221 gcctcgcgac ctaccggccg aacaagatcc tcggttccgt cctttcagtt caaaaaaccg
    23281 aagacttcgt ccaggacgac gtcaaccggc gcatccaggt gaaatcggtc ccggcgccgg
    23341 tgctcacctc gctgcgctgg cccggccgcc ccgacctctc cggcggcaac ccggcgtgga
    23401 cctacatgat cgagcacccg cagtaccgct tcgcggtgtt cgtcggtcat gttgagaatg
    23461 gcagacccca cgcattcgaa gtctgggtca atggcagcga gcagccgcgc ggcctcggcg
    23521 cgctcgccaa gacgctgtcg atggacatgc gcgcgagcga cagcgcctgg ctcaagctca
    23581 agctcgacgc cctcgccaag gcgggcggcg acgagccctt cgaaatgccg ttcccgccgc
    23641 gcggcgagaa gaagctgatg ccgtcggtgg tgtccgcctt cgcccaggtg gtgcgctggc
    23701 gggtcgagca gctcgcctct ggtttaacag aggaaccgtt tcaattggcg g
//
LOCUS       DATBSZ010000290        10534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2040098, whole genome shotgun sequence.
ACCESSION   DATBSZ010000290 DATBSZ010000000
VERSION     DATBSZ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2040098"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VIG70_18895"
     CDS             <1..237
                     /locus_tag="VIG70_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY8252665.1"
                     /translation="SFTHAMTAVSAVIFLATAKYNLATVYIIGRVEAGEYPLAIAYST
                     VLILLMLAMLLVMQKVIGETRLGRRTASLVVTGH"
     gene            244..1296
                     /locus_tag="VIG70_18900"
     CDS             244..1296
                     /locus_tag="VIG70_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8252666.1"
                     /translation="MNRQEGGVVFESVSKFFGAVAAVDNVSFDVASGELVTLLGPSGC
                     GKTTTLRMVAGLESASQGRVLIGGVDVTRLPANERDVSMVFQSYALFPHMNVLENVAY
                     GPTVSGDRAAKEKALEKLRMVGLNAFASRMPSELSGGQQQRVAVARALVLEPQVLLFD
                     EPLSNLDAKLRRRVREEIRELQQSLGLTVAYVTHDQQEALAVSDRIIVMSNARIAQIG
                     EPRELYEAPRDRFVADFIGDANLVDAELGAHRGSRAPVRLGGLEVELEHRGVAPGAVR
                     VAIRPESILLKRDGSSQLRGTISKSAYLGTHMEYTVTAPVGQLFVVDRDVSRPIPAGA
                     EVSILLSDHGVALVPS"
     gene            1314..4910
                     /gene="hrpA"
                     /locus_tag="VIG70_18905"
                     /pseudo
     CDS             1314..4910
                     /gene="hrpA"
                     /locus_tag="VIG70_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
     gene            4907..5662
                     /locus_tag="VIG70_18910"
     CDS             4907..5662
                     /locus_tag="VIG70_18910"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEY8252667.1"
                     /translation="MSILGALGYGLVNVFHPRMLWLMVWPMLVALALWGSVALVMWTR
                     LALWLAELLNQWALGIVRVDLGQLAVFLANVALLLLFVPLVYLTALFILGAFGMQKMV
                     DHVATRSFAHVERRRGGSTAGSVWNAVVALIGMLLLFAVSLPLWLIPVLWPVITLAIL
                     TWVNQRLLRYDALAQHADAAEMAQLFRQHRGPLLVLGFLLGLLAYVPFVGFFVPVVFG
                     LAFIRYLLGALESIRQGYRAHPNKELAAGNAPP"
     gene            5696..6433
                     /locus_tag="VIG70_18915"
     CDS             5696..6433
                     /locus_tag="VIG70_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY8252668.1"
                     /translation="MLQNREGQKVPQASFRTRTDSDWKSITTDELFKGKTVVVFSLPG
                     AFTPTCSSTHVPRYNELAPAFFANGVDRILCVSVNDAFVMNEWARTQECGNVLMVPDG
                     NGEFTEKMGMLVDKTELGFGKRSWRYSMLVKDGVIDRMFIEPEKPGDPFEVSDADTML
                     DYINPKAKKPDQVAILTREGCGYCAKAKALLKDLGYAFSEVSLDHSDRSRVVGAISGR
                     GTVPQIFVNGKHIGGLEDLQRWAKKAA"
     gene            6434..7171
                     /locus_tag="VIG70_18920"
     CDS             6434..7171
                     /locus_tag="VIG70_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY8252669.1"
                     /translation="MFGAFVIGDEILVGKREDKHLSFLIAALAKRGLRLAWAQYLGDD
                     PERLTAALRRSLDSEDVVFSFGGIGATPDDHTRQCAAAALNLELRIHPEAEREIRARF
                     GGEVTPQRLQMGEFPVGSSIIPNPVNRIPGFSLREHHFVPGFPQMAWPMAEWVLDTKY
                     QKLFNRDRWAESSILVFEAGESQLIPAMQAVEAGFKGIKVFSLPSMGKDGSRIHVELG
                     VRGDPEQVEPAMERLRRLVRDAGFPYK"
     gene            complement(7252..7794)
                     /locus_tag="VIG70_18925"
     CDS             complement(7252..7794)
                     /locus_tag="VIG70_18925"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY8252670.1"
                     /translation="MYVTPEQIQTANKTSVEALLAVANAQFAAFEKLAHISAGAVKTA
                     FEDSIANTRALLSAKDVQEFVALQNSFAQPAMEKAIAYSKSVYEVATEANTELSKVAE
                     RRVAEWNEGFVSLLDKVSKNAPAGSDVAVAAIKSMLAASNSAYDNFTKVAKQATEMAE
                     ANVSAATETVKGLAKAKKAA"
     gene            7965..9140
                     /locus_tag="VIG70_18930"
     CDS             7965..9140
                     /locus_tag="VIG70_18930"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY8252671.1"
                     /translation="MAEPFGLYARMRDEDPAHWSPRLKAWVLTRYEDVKRVCLDTTMS
                     SDRLRPFFATLPPPEAAKVAELSRYLTLWMVFRDPPEHTRLRRLAAKVFHVRSMHALR
                     PNVERLTHWLLDSLSEKQEFDFIADFAGPLPALVIMDMLGAPREELARMKRLSDEMAL
                     FIGSARDASGKYERASAATREMAGIFRELVSARRAAPQRDLLSELVALDDAGDRLSDD
                     ELVATCILLLFAGHETTTHHLANGLRALLAFPDELEKLRADRSLAPAAVEELLRYDGP
                     IGAQVRIVQEPHALHGKPLQAGDRVFLLMNAANRDPRAYDDPERLDLERNGVPHLTFG
                     FGAHICLGFPLARLEGQIALPAVLGRWRHLELAGEPLEWMDSMVLRGMKAMRLRARA"
     gene            9137..10039
                     /locus_tag="VIG70_18935"
     CDS             9137..10039
                     /locus_tag="VIG70_18935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8252672.1"
                     /translation="MSAEPDHLPQRLWWVLAALTLAWGFNWTALKVGLSEVPPWTFRS
                     LCLWLGAAVLFIALRAGGQRLAPPSGQWGRLWLFALLNITCWNMLVAYGVAMIPSGRA
                     AILAYTMPVWAVPLSVWFLRERITGAKLFGLALGLGGLALLLLESFVGLGRAPLGALL
                     VLGASVTWALGTVLQKRFPVSMPTGSYTVWIMLLGGAPILVGAVLFEDWHALKEVGVA
                     AWLGTAYNVFIAFAFAHWAWLKIATSVPVSVFSISMLLIPVVGVVSGILLLGERPTWA
                     EYASLALVLGALLTVLRPGRGARG"
     gene            complement(10002..>10534)
                     /locus_tag="VIG70_18940"
     CDS             complement(10002..>10534)
                     /locus_tag="VIG70_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEY8252673.1"
                     /translation="DGAQIYPGTCRNGIAPDRTPRALRLRTSAERIVFDDLLQGRQQQ
                     ILERDVGDFVLHRADGQFAYQLAVVVDDAEQGVTDVVRGADLLDSTARQICLQRLLGY
                     PQPRYLHVPAAVNAAGEKLSKQTGAPPIDAGRRRDELARALAFLGQPPADDLTQAVQN
                     WDPALIPARRALAAAP"
BASE COUNT         1628 a         3516 c         3547 g         1843 t
ORIGIN      
        1 agcttcacgc acgcgatgac cgcggtcagc gcggtgatct tcctcgccac ggccaagtac
       61 aatctcgcca ccgtctacat catcggccgc gtcgaagcgg gcgaataccc gcttgcgatc
      121 gcctactcga cggtgctgat cctgctcatg ctggcgatgc tgctggtgat gcagaaggtg
      181 atcggcgaaa cccgcctggg tcgccgcacc gccagtctcg tggtgaccgg acactaagct
      241 gacatgaatc gacaggaagg tggcgtcgtc ttcgaaagcg tctcgaaatt cttcggcgcg
      301 gtggccgccg tcgacaacgt gtcgttcgac gtggcgtcgg gcgagctcgt cacgctgctc
      361 ggcccctcgg gctgcggcaa gaccacgacg ctgcggatgg ttgccgggct ggaatcggcc
      421 tcgcaaggac gcgtgctgat cggcggcgtc gacgtgacga ggctgccggc gaacgagcgc
      481 gacgtcagca tggtgttcca gtcctacgcg ctgttcccgc acatgaacgt gctcgagaac
      541 gtcgcctacg ggccgacggt gagcggcgac cgcgcggcga aggagaaagc gcttgagaag
      601 ctgcgcatgg tcggcctcaa cgcgttcgcg agccgcatgc cttcggagct ctccggcggc
      661 cagcagcagc gcgtcgcggt ggcgcgcgct ctggtgctcg agccgcaggt gctgctgttc
      721 gacgagccgc tctccaacct cgacgccaag ctgcgccgcc gcgtgcgcga ggagatccgt
      781 gagctgcagc agagcctcgg cctgacagtc gcctacgtca cgcacgacca gcaggaggcg
      841 ctcgccgtgt cggatcgcat catcgtgatg tcgaacgcgc gcatcgccca gatcggcgag
      901 ccgcgcgagc tctacgaggc gccgcgcgac cgcttcgtcg ccgatttcat cggcgacgcg
      961 aacctggtcg acgccgagct cggcgcgcac cgcggcagcc gcgcgccggt ccgcctcggc
     1021 ggcctcgagg tcgagctcga gcaccgcggc gtcgcgccgg gcgccgtccg ggtcgccatc
     1081 cggccggagt cgattcttct gaaaagagac ggttcttccc agctgcgcgg aaccatcagc
     1141 aagtccgcct acctgggcac gcacatggaa tacacggtca ccgcgcccgt cgggcagctc
     1201 ttcgtcgtcg accgcgacgt gtcgcgaccg attcccgccg gcgccgaggt ctccatcttg
     1261 ctttccgacc acggcgtggc gctcgtccca agctgacgat ccatttcgac cccgcgctgc
     1321 cgatcaacgc gcggcgcgac gagatccgcg ccgcggtcgc ggcgcacccg gtagtcatcg
     1381 tgtgcggcga gaccggctcg ggcaagacca cgcagctgcc gaagatcctg ctcgagatgg
     1441 atccacgcct gcgcatcggc cacacccagc cgcggcgcat cgccgcgcgc tcggtcgccg
     1501 cgcgcatcgc cgaggagctg ggaaccgagc tcggcgaccg ggtgggctac aaggtgcgct
     1561 ttcacgacca ggtgggcaag cgcaccgcga tcaagctcat gaccgacggc atcctgctcg
     1621 ccgagactca gggcgaccgc gagctgcgtc agtacgaccg gctgatcatc gacgaggcgc
     1681 acgagcgcag cctcaacatc gattttcttc tgggttacct cagaggcctc ctcgagaggc
     1741 ggagcgacct caaggtgctc atcacttcgg cgaccatcga cgccggccgc ttctccaggc
     1801 acttcggcga tgcgccggtg atcgaggtct cgggccggct ctatccggtc gagatgcgct
     1861 accgcccggt cgccggcgac gccgaggaca cgactcgcga agaggaggag caggcgctcg
     1921 ccgacgcggt cgacgagctc tgccgcgaag gccccggcga cgtgctggtg ttcctgcccg
     1981 gcgagcgcga gatccgggac gccgcggaca ccctaaggag gaagggcccg aaaggtgtcg
     2041 agctgctgcc cctctactcc cggctgtcca ccgcggagca ggaccgcgtc ttcaagccgg
     2101 gaaaggaacg ccgcatcgtg ctcgcgacca acgtcgccga gacctcgctg accgtgccgc
     2161 gcatccgcta cgtcgtcgat accggcgacg cgcgcgtcaa gcgctacagc taccgcaaca
     2221 aggtcgagat gctgcgcgtc gagccgatct cgcaggccgc cgcgcagcaa cgcgccgggc
     2281 gttgcggccg cgtcgccgac gggatctgca tccgcctgta ttcggaggag gacttcgcga
     2341 agcgcccggc ttttaccgac ccggagctgc tgcgctcctc gctcgcttcg gtgatcctgc
     2401 gcgcgaaaag cctcgggctc ggcgaagtcg aggcgtttcc gttcgtcgat ccgcccaacc
     2461 cccgcgccat cgccgatggc tatgacctgt tgacggagct cggtgcggtc gacgaccaca
     2521 gaaacctcac ttcgataggg ctggagctcg cccgcctgcc gctcgacccg cgcgtcgcgc
     2581 gcatgctggt ggcggcgcgc gaggagggca gcctcgcgca ggtccgcatc atcgccgcag
     2641 cgctgtcggt gcaggatccg cgccagcggc cgctcgagcg cgcggcggcg gccgacgatc
     2701 gccacgcgcg tttcgccgac gagcagtcgg acttcctctc ctttctcaag ctgtggaagc
     2761 tgcaggacga gccgggcctg cggcggacgt gccgcgacaa cttcctctcc tatccccgca
     2821 tgcgggaatg gcgcgacgtg cacgagcagc tgaagcaggc gttggactgg ccggaatcct
     2881 ctgtaagaaa cgacaaagcc gaaggctacc gtgccatcca tcgagccctg ctcgccgggc
     2941 tgctcggcaa cgtcggcatg cgcgacgaag aaggcgctta cacgggagcg cgcggcatca
     3001 agttccgggt gcatcccggc tcgtggacca agaaaccggg caagtggatc gtcgccgccg
     3061 agctggtgga gaccacgcgg ctcttcgcgc gcaccgtcgc cggcatcgag ccgcgctggc
     3121 tggaggagat cgggtcgcac ctcctgaagc gcagccgcag cgacccgcac tgggagaagg
     3181 gccgcggcga ggtcgtggcg ctcgagcgcg gcacgctgta cggccttccg gtatacgtga
     3241 accggcgagt gccctttgcg ccgttcgatc ccgatctggc gcgcgacatc tttatccgct
     3301 cggccctggt cgggggcgag ctcgatacgc gcgcgccctt cttcgcgcac aaccggcgcc
     3361 tggtcgccga tatcgagcgc ctcgagcaca agtcgcgccg gccggacatc ctggtcgacg
     3421 acgagctgat ccacgcgttc tacgacgagc ggattcccaa ggaaatcaat tcttcagaag
     3481 cattcgaacg ctggcgacgc caggcggaag ccgcgcggcc gcggctgctc catctcgcgc
     3541 gcgaggacct gatgcgccac gaggctgcgg gcatcaccac gcagaacttt ccgcccgcgc
     3601 tcgagctcgg accgaaccgc tatcccctcg agtaccactt cgagcctgga tcggcgcgcg
     3661 acggcgtgac gctgaacgtg ccgctcgcgc ttctcaacca ggtgccggcc gcccggaccg
     3721 aatggctggt gcccggtctg ctgaaagaaa aagtcaaagc gctggcgaag tcgatgccgc
     3781 agcgcctgcg gcacaagctc ggggcgctgg acgacttcgc tgcgcagttc gccgcagccg
     3841 tcgcgccgtc gggtctgccg ctggccgagg cgttgcgccg ctacgtgcgc gatgagctga
     3901 acctcgacgt tccgctcgac gccttccggc ccgattcggc gccgccgcac ctgcacatga
     3961 actttcgcgt tctggacgaa gacggccgtc agctcgacat ggggagaaac ctgccggagc
     4021 tgaagcgcgc gctcgggggg aagaccgagg cgattcttca gcaggaagcc cccgtcgatg
     4081 aaggagagcg ctacacgggg tggaccatgg gcgatctgcc cgagctcatg gagatcagcc
     4141 gcggcggcca taccctcatg gggtatccgg cgctggtcga cgccggggat gcggtcacat
     4201 tgcaggtgtt cgattcgccg gagaaggcgc gcgagctgca ccgtgccggc gtgctgcggc
     4261 tgctcgcgat cgcgttccgc gaccggatcc gcgatatcga gcgctcgctc gccaaggatg
     4321 tcgtcctcgg gcccttgaaa gccgacgcgg tagcggccgc gctcgggcgc acctttctgc
     4381 agcaatccgt tccaacgctg caggccgagt tcgcgcggcg gctcgaggag gggcgcagcc
     4441 ggttcaatct gatcgcgcag gagatcgcgc gcaccgcggc cgggattctc gccgagcgcg
     4501 cggcgctgga gaagaaactg aacgcgatga gcaagggctt tccggggcaa accgacgatg
     4561 tgaaacagca ggtgcagcgg ctgctggctc ccggttggct cgcgcacgcg ccctgggagc
     4621 gcctgcagca tttcctgcgc tatctcaagg ccgcgtcgct gcggctggac aagctgcgcg
     4681 cggatcccgc gcgcgacgcg cgcctcgccg ccgagctcgc gccgctcgag caggcttacc
     4741 gccgcgaggc tgcggcgcgc gcccgggaag gcgcgcccgg cgcggagttc gagcagttcg
     4801 gctggctgct cgaggagctg cgcgtgtcac tgttcgccca ggagctgaaa acgcctgtgc
     4861 ccgtctccgt caagcggctg acgaaactct ggcagactgt ccgccgatga gcatcctcgg
     4921 cgcgctgggc tatgggctgg tcaacgtgtt ccaccctcgc atgctgtggc tcatggtttg
     4981 gccgatgctg gtggcgctgg cgctctgggg atcggtcgcg ctggtgatgt ggacgcggct
     5041 cgcgctgtgg ctcgccgagc tgctcaacca gtgggcgctc ggcatcgtgc gcgtcgacct
     5101 cgggcagctc gccgtattcc tggcgaacgt cgcgttgctc ctgctgttcg tgcccctggt
     5161 ctatctgacc gcgcttttca tccttggggc tttcggaatg cagaagatgg tggatcacgt
     5221 agccacgcgc tctttcgcgc acgtggagcg ccgacgcggc gggagcacgg caggcagcgt
     5281 ctggaacgcc gtggtcgctt tgattggaat gctgctgctg ttcgcggtca gcctgccgct
     5341 gtggctgatc ccggtgctat ggccggtgat cactcttgcc atcctgacct gggtcaacca
     5401 gcgcctgctg cgctacgacg cgcttgccca gcatgccgac gcggccgaga tggcgcagct
     5461 cttccgccag caccgcggtc cgctgctggt gctcggcttc ctgctcggcc tgctggccta
     5521 cgtgccgttc gtcgggttct tcgtcccggt ggtgttcggc ctcgccttca tccgctatct
     5581 gctcggcgcc ctggagtcga ttaggcaggg ctatcgcgct catccgaaca aagaattggc
     5641 cgcggggaac gctcctccct agactcggtc cattcttttt tcgagaggaa tcaacgtgct
     5701 tcagaatcgc gaaggccaga aagttcccca ggcaagcttc cgtacgcgca cggacagcga
     5761 ctggaaaagc atcacgacag acgagctttt caagggcaag accgtggtgg tgttctcgct
     5821 gccgggcgcg tttaccccga cctgctcatc gacccacgtg ccgcgctaca acgagctcgc
     5881 gccggcgttc ttcgccaacg gtgtggaccg catcctttgc gtctcggtca acgacgcctt
     5941 tgtcatgaac gaatgggccc gcacccagga atgcggcaac gtcctgatgg tgccggacgg
     6001 taacggcgaa ttcaccgaga agatgggcat gctggtcgac aagaccgagc tcggcttcgg
     6061 caagcgctcg tggcgctact cgatgctcgt gaaggacggc gtgatcgata ggatgttcat
     6121 cgagccggaa aagcccggcg acccattcga agtttccgac gccgacacga tgctcgacta
     6181 catcaatcca aaggcgaaga agcccgacca ggtggcaatc ctcacgcgcg aaggctgcgg
     6241 ctactgcgca aaggccaagg cgctgctgaa ggacctcggc tacgcgtttt ccgaggtcag
     6301 cctggaccac agcgatcgct cacgcgtcgt cggggcgatc tcggggcgcg gcaccgtgcc
     6361 gcagatcttc gtgaacggca agcatatcgg cggcctggaa gacctccagc gctgggcaaa
     6421 aaaggcagcc tgattgttcg gcgctttcgt catcggggat gaaatcctcg tcggaaagcg
     6481 cgaagacaag cacctttcct tcttgatcgc agccctcgcc aagcgggggc tgcgccttgc
     6541 ctgggcgcaa tacctcggcg acgacccgga gcggctcact gcggcgctgc gccgcagcct
     6601 ggattcggag gacgtggtgt tcagcttcgg cggcatcggc gccacgcccg acgaccacac
     6661 gcggcaatgc gccgcggccg ctctgaatct cgaattgaga attcacccgg aggcggagcg
     6721 cgagatccgc gcccgcttcg gcggcgaggt cacgccgcag cgcttgcaga tgggcgagtt
     6781 ccccgtgggc tcgagcatca ttcccaaccc cgtgaaccgc atccccggct tctcgctgcg
     6841 cgagcaccac ttcgtgcccg gctttccgca gatggcctgg ccgatggcgg aatgggtgct
     6901 ggatacgaag taccagaagc tcttcaaccg ggatcgatgg gccgagagct cgatcctcgt
     6961 gttcgaggcg ggagagagtc agctgatccc ggccatgcag gcggtcgagg ccgggttcaa
     7021 gggcatcaag gtattcagcc tgccgtcgat gggcaaggac ggaagccgga tccacgtcga
     7081 gctcggcgtg cgcggcgatc cggagcaggt cgagccggca atggagaggc tgcgccggct
     7141 ggtacgcgac gccgggtttc cgtacaaata aaaagcccgc ctatcggcgg gctttcttgt
     7201 gcttcttgcg ctttacgcta gattgggcct ttttgcgacc cggtttggcg tttaggcggc
     7261 tttttttgct ttggcgaggc ccttcacggt ctcggtcgcg gccgatacgt tggcttccgc
     7321 catttcggtc gcctgcttcg cgaccttggt gaagttgtcg tacgcggagt tgctcgcggc
     7381 cagcatcgac ttgatcgcgg cgacggcgac gtccgagccg gcgggagcgt tcttcgacac
     7441 cttgtcgagc aggctgacga agccttcgtt ccactcggcg acgcggcgct cggcgacctt
     7501 gctgagctcg gtgttcgcct cggtggcaac ttcgtatacg ctcttcgagt aggcgatggc
     7561 cttttccatg gcgggctggg cgaaggaatt ctgcagcgcc acgaattcct ggacatcctt
     7621 cgcgctgagc agcgcacgag tgttggcgat cgagtcttcg aaggcggttt tcacggcgcc
     7681 ggcgctgatg tgggccagct tctcgaaggc cgcgaactgc gcgttcgcca cggcgagcag
     7741 ggcttcgacg ctcgtcttgt ttgcggtttg aatctgttcc ggggttacgt acatttcaat
     7801 ctcctgagtc tgtctgaatg cggttatggt gcaatgcagc aaaacggatt gtaggccgat
     7861 ggcgactgaa gtcaagaagt ttgttgcgta gcagcaatca ctacaaagca aaaggattta
     7921 agttagtgct cgctatcgat tacaagccta atgatccgac catcttggcg gagccatttg
     7981 ggctgtacgc caggatgagg gacgaggacc cggcgcactg gagcccgcgg ctcaaggcct
     8041 gggtgcttac ccgctacgag gacgtcaagc gcgtttgcct ggacaccacc atgtcgtcag
     8101 accgcttgcg ccccttcttt gccacgctgc cgccgcccga ggcggcaaag gtcgccgagc
     8161 tttcccggta tctcaccctg tggatggtgt tccgcgaccc tccagagcac acgcggctgc
     8221 ggcggctcgc ggcgaaggta ttccacgtgc gctcgatgca tgccttgcgc cctaacgtcg
     8281 agcgcctcac gcactggctc ctggattcgc tttctgaaaa acaggaattt gacttcatcg
     8341 ccgacttcgc tgggccgctg cctgcgctgg tgatcatgga catgctcggc gcgccgcgcg
     8401 aggagctcgc gcgcatgaag cgcctgtcgg acgagatggc gcttttcatc ggcagcgcgc
     8461 gcgacgcgtc cggcaagtac gagcgcgcct cggccgcgac ccgcgagatg gcggggatct
     8521 tccgcgagct ggtgagtgcc cggcgcgccg cgccgcagcg cgaccttctg agcgagctgg
     8581 tggcgctcga cgatgccggc gaccggctct ccgacgacga gctggtcgcg acgtgcatcc
     8641 tcctgctgtt cgccggccac gagactacga cccatcacct cgcgaacggg ctgcgcgcgc
     8701 tgctcgcttt cccggacgag ctggagaagc tgcgggccga tcggtcgctc gcgccggcgg
     8761 cggtcgagga gctgctgcgc tacgacggac cgatcggcgc acaggttcgc atcgtccagg
     8821 agccgcatgc gttgcatggc aagccgctcc aggcggggga ccgcgtgttc ctgctcatga
     8881 atgccgccaa ccgggacccg cgggcctacg acgatcccga acgactcgat ctcgagcgca
     8941 atggcgtgcc gcatctcacg tttggcttcg gcgcgcacat ctgcctcggc tttccgctcg
     9001 cccgtctcga aggccagatc gcgctgcccg cagtgctcgg gcgctggcgg catctcgagc
     9061 ttgccggcga gccgctcgag tggatggatt ccatggtact gcgcggaatg aaggcgatgc
     9121 ggctgcgggc gagggcttga gcgccgagcc cgatcacctg ccgcagcgcc tgtggtgggt
     9181 gctcgccgcg ctcacgctcg cgtgggggtt caactggacg gcgctgaagg tggggctttc
     9241 ggaagtgccg ccctggacct tccgcagcct ctgcctgtgg ctgggcgccg ccgtgctgtt
     9301 cattgccctg cgcgccggcg gccagcgcct cgccccgccc agcggccaat ggggccggct
     9361 ctggcttttc gcgctgctca acatcacgtg ctggaacatg ctggtggcct acggcgtcgc
     9421 catgatcccg tcggggcgcg cggcgatcct tgcctacacc atgccggtct gggcagtgcc
     9481 gttgtcggtg tggtttctcc gcgagcgcat caccggcgcg aagcttttcg gtctcgcgct
     9541 gggcctcggc gggctggcgc tcctgctcct cgaaagcttc gtcggcctcg gccgcgcgcc
     9601 gctcggcgcg ctcctggtgc tcggcgcctc ggtgacctgg gcgctcggca cggtgctgca
     9661 gaagcgcttc ccggtcagca tgccgaccgg gtcctatacc gtgtggatca tgctgcttgg
     9721 cggcgcgccg attctcgtcg gcgccgtcct gttcgaggac tggcatgccc tgaaagaggt
     9781 cggcgtggct gcctggctcg gcaccgctta taacgtgttc atcgccttcg ccttcgcgca
     9841 ctgggcgtgg ctcaagatcg cgacctcggt gccggtgagc gtgttctcga tcagcatgct
     9901 gctcatcccg gtcgtcggcg tggtgagcgg catcctgctt ctcggcgagc ggccgacctg
     9961 ggccgaatac gcctcgctcg cgctcgtgct gggcgctctg cttacggtgc tgcggccagg
    10021 gcgcggcgcg cggggatgag cgccgggtcc cagttctgga ccgcctgtgt gaggtcgtcg
    10081 gccggaggct ggccgagaaa cgccagcgcg cgcgccagct cgtcgcgtcg tcgtccagcg
    10141 tcgatcggcg gcgcgccagt ctgcttcgag agcttttcgc ccgccgcatt caccgccgcc
    10201 gggacgtgca ggtagcgcgg ctgcggatag ccgagcaggc gctgcaggca gatctgccgc
    10261 gccgtcgagt cgagcaggtc ggcgccgcgc accacgtcgg tgacgccttg ttcggcgtcg
    10321 tccaccacca cggcgagctg ataggcgaac tgcccgtcgg cgcgatgcag cacgaagtcg
    10381 ccgacgtcgc gctcgaggat ctgctgttga cggccctgga ggagatcgtc gaacacgatg
    10441 cgttccgcgg aggttctcaa tcgaagcgca cgcggcgtcc tgtccggcgc gatcccgttg
    10501 cggcaggtgc cgggatagat ctgcgcgccg tcgg
//
LOCUS       DATBSZ010000291        14039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1287890, whole genome shotgun sequence.
ACCESSION   DATBSZ010000291 DATBSZ010000000
VERSION     DATBSZ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14039
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287890"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..599
                     /locus_tag="VIG70_18945"
                     /pseudo
     CDS             93..599
                     /locus_tag="VIG70_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
     gene            611..1186
                     /locus_tag="VIG70_18950"
     CDS             611..1186
                     /locus_tag="VIG70_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEY8252674.1"
                     /translation="MIRRDSLLSLEAYARGRNEFRARVIEHKKRRTLHIGEHVTLQFE
                     DELLIRYQIQEMLRVERIFEDDGIQGELDAYNPLLPDGGNWKATMFIEYTDVEQRKRM
                     LERLKGVERRTWVQVGDAERIYAIADEDLERENEEKTAAVHFVRFELPKALREALRRG
                     ARLTIGVDHPAYRAVLEPSPEQRAALLADLA"
     gene            1183..1701
                     /locus_tag="VIG70_18955"
     CDS             1183..1701
                     /locus_tag="VIG70_18955"
                     /inference="COORDINATES: protein motif:HMM:NF020331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEY8252675.1"
                     /translation="MRRLLGAAALALAAACGSPSVEKSDWERENETRLLIPEETPAPP
                     AYPARGQLVRFFTLERSDFNFFVDASSISIGKDGVVRYVLVARSPEGADNVTFEGMRC
                     QKPELKVYAVGRDGGWAGRPGAWRAFETGRTQPWHLVLYRDFFCPGRAPISSPEKAAA
                     VLRRGGARGMTD"
     gene            complement(1698..2807)
                     /gene="proB"
                     /locus_tag="VIG70_18960"
     CDS             complement(1698..2807)
                     /gene="proB"
                     /locus_tag="VIG70_18960"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY8252676.1"
                     /translation="MIEQAKTLVVKVGSSLVTNEGRGLDAAAIGRWAGQVAQLNALGK
                     KVILVSSGAIAEGMQRLGWSKRPHAMHELQAAAAVGQMGLAQCYESCFRSHGLHSAQV
                     LLTHADMADRQRYLNARSTLRTLLGLGVIPVINENDTVVTDEIKLGDNDTLGAMVTNL
                     VEADALIILTDQAGLFDADPRKNENAKLLKEVDASDPALEALAGGAGSAIGKGGMLTK
                     VLAARRAARSGAHTVIASGAEPEVLLRLARGERVGTLLTAQTVPLAARKQWLADHLTV
                     AGQVRLDAGAVRALVRDGKSLLPIGVTEVTGEFQRGEVVTVLDPAGREVARGLANYSA
                     EEARRIIRRPSGEIEAILGYVDEPELIHRDNLVLL"
     gene            complement(2812..3798)
                     /gene="obgE"
                     /locus_tag="VIG70_18965"
     CDS             complement(2812..3798)
                     /gene="obgE"
                     /locus_tag="VIG70_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY8252677.1"
                     /translation="MKFIDEAKIEVHAGKGGDGAATFRREKYIRYGGPNGGDGGRGGS
                     VWARADRNINTLVDYRFARIHRAENGVRGTGSDCDGRAGRDVELRVPVGTLIRDEDDG
                     ALVADLARDGERALLARGGQGGLGNVHFKSSVNRAPRQFTRGEPGESRDLTLELRVLA
                     DVGLLGLPNAGKSSLIRAVSSARPKVADYPFTTLDPSLGVVRVGEASFVVADIPGLIE
                     GASEGAGLGHQFLRHLARTRLLLHLVDLTEPDLVGGARIIAAELKKYGQGLERKPRWL
                     VFNKIDIVDDADARIQRVLRALRWKRPWFKVSALSGEGCAAVSKAAARELAK"
     gene            complement(3889..4161)
                     /gene="rpmA"
                     /locus_tag="VIG70_18970"
     CDS             complement(3889..4161)
                     /gene="rpmA"
                     /locus_tag="VIG70_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY8252678.1"
                     /translation="MAHKKAGGSSRNGRDSQSKRLGVKVYGGERVNAGQILVRQRGTK
                     VHAGVNVGMGRDHTLFARVSGQVQYAQRGADQNNTVSVIAEKSPAN"
     gene            complement(4164..4478)
                     /gene="rplU"
                     /locus_tag="VIG70_18975"
     CDS             complement(4164..4478)
                     /gene="rplU"
                     /locus_tag="VIG70_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY8252679.1"
                     /translation="MYAVIKSGGKQYRVESGKQVRVETLAADVGAAVAFEEVLLVGTG
                     DSVKVGAPLVSGAKVKATVVAHGRGEKVKIFKLRRRKHYQKSQGHRQNYTEVRIDDIV
                     QG"
     gene            4536..5534
                     /locus_tag="VIG70_18980"
     CDS             4536..5534
                     /locus_tag="VIG70_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY8252680.1"
                     /translation="MSLLSTLAPVADDLARVDALIRRRLDSDVALVRQVAEYLVAAGG
                     KRLRPALLLLACGALGYKDRKSSGNGGSEARLMLAAVVEFIHTATLLHDDVVDASDLR
                     RGRTTANAVFGNAPAVLVGDFLYSRAFQMMVEVDDMRVMQVLAEATNTIAAGEVMQLM
                     GSHDPEVDEARYLEVIRRKTAKLFEAAARLAAVLARQGNFEQPLATYGRHVGTAFQLI
                     DDVLDYGGELGKSLGDDLAEGKPTLPLIYTMRCGSAQQAAVVRRAIAEGGREDFASVL
                     EAIRACGALDYARVAAQREVDAATRAIGALPESEYKRSLLELAAFAATRSSNPIGA"
     gene            5525..5601
                     /locus_tag="VIG70_18985"
     tRNA            5525..5601
                     /locus_tag="VIG70_18985"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5559..5561,aa:Pro,seq:cgg)
     gene            5656..5889
                     /locus_tag="VIG70_18990"
     CDS             5656..5889
                     /locus_tag="VIG70_18990"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEY8252681.1"
                     /translation="MGRLIVLILLVVLAVWLIRRALRASSRAEPARKESPAQQDLIRC
                     ARCGVHLPRAEARLAGGLLYCGEEHARLGAGEG"
     gene            5889..7457
                     /locus_tag="VIG70_18995"
     CDS             5889..7457
                     /locus_tag="VIG70_18995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8252682.1"
                     /translation="MEASVRAPVTLSERFAPPEEGVPESFWISLRYFNLYRIALAGLF
                     LGTAVIYGDALNLGTHNLALFRYFAAGYLLLGVVFHGVLRDQRDRFELQLTLQAGLDI
                     VAITLLMYASGGIRSGLGVMLLISLTGAAIVAPRRLTFLYAALATIALLLEQSYWVLE
                     HDAPTASYVQPGLLAIGYFVAAGVTSWLAARVAANERLARQRGRELARQTQVNQLVIE
                     DMNEGVLVLDRGGTVVQHNPQAQELLGAAGLIGAAIASVVPGFSGSGEFEVAGRDIRL
                     RPLEASAEEFSVWLVEDTTRAREQARQLKLAALGRLTANIAHEIRNPLAAISHATELL
                     REEKRGDDRERLTRIIHDNAGRLERLVSDVLQLNKRDRVSAAPLRLHQWLGAFITEFV
                     ANEAVAQGRIVLETRRESWIEFDHEHLHQVMWNLLRNAVRYAPAEPGSIRIRLDGYAD
                     RVELSVIDSGPGVPAAIQGQLFEPFFTTEAKGTGLGLYLARELCAANRAVLEYVNGGA
                     GGHFRIRCRQAASK"
     gene            7489..8889
                     /locus_tag="VIG70_19000"
     CDS             7489..8889
                     /locus_tag="VIG70_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8252683.1"
                     /translation="MTRRPERRATQRDAKVLVVDDEPDIRELLELTLARMGLGVDAVG
                     SIAEAKERLKTERYDLCLTDMRLGDGEGLELVRHITTLAADLPVAVITAYGSADNAVA
                     ALKAGAFDYVSKPVGLEQLRALVKSALSLPERAEGAGDTAGRQLLGESAPLAQVRELI
                     GKLARTQAPVYISGESGSGKELAARLIHENGARREKPFVAVNCGAIPDTLVESELFGY
                     RKGAFTGANEDRDGFFQAAHNGTLFLDEIAELPLHTQVLLLRAIQERRVRKVGSTQEE
                     PVDVRIICATNQSLAGMVEAGRFRRDLYYRLNVVDLTMPPLRECREDIPLIATHILQR
                     LSAQNGVPAPSLSAPALEELMAYDFPGNVRELENILERALALSPGQELGPADLALHRI
                     APEEEDASPPGEGQPARPADSPLPAYLDQLEREAILQALGKTGFNRTAAARLLGITFR
                     QLRYRMQRLNIKDENR"
     gene            8876..9409
                     /gene="ampD"
                     /locus_tag="VIG70_19005"
     CDS             8876..9409
                     /gene="ampD"
                     /locus_tag="VIG70_19005"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEY8252684.1"
                     /translation="MRIVDSPNCDERPAGVEVSLVVLHSISLPPGEYGGDSIERLFTN
                     CLDPQAHPYFQEIRGVKVSSHFLIRRDGEVVQFVPVERRAWHAGASAWRGRSRCNDFS
                     IGIELEGTEDDAFTDAQYRALTSLLKKLRTRLPLRDVAAHSDVAPGRKTDPGARFDWA
                     RLLAGLAKSRAAPVSQH"
     gene            9698..12451
                     /locus_tag="VIG70_19010"
                     /pseudo
     CDS             9698..12451
                     /locus_tag="VIG70_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116304.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
     gene            12505..12882
                     /locus_tag="VIG70_19015"
     CDS             12505..12882
                     /locus_tag="VIG70_19015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252685.1"
                     /translation="MSVITLGSRSFAGPFMAPLWSPPKGAGLYAVMAPGWRLLTFRAL
                     DFERAGDLAEPQLLKKHPKYREWIGIVGTEWNLYIATCEMAVSTEAEREAALRTLLRE
                     YRPEFKPLSPDPVPPRGQLKPTC"
     gene            12876..13982
                     /locus_tag="VIG70_19020"
     CDS             12876..13982
                     /locus_tag="VIG70_19020"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEY8252686.1"
                     /translation="MLNFEDLTSAPIPGATPVRREAPPALDAALRRVTVDDKKIINSK
                     ADVNQLVPFKYKWAWEKYIAACANHWMPQEINMSRDIELWKDPNGLTEDERRVIKRNL
                     GFFVTADSLAANNIVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQYIVESLDLDESE
                     IFNAYHEVTSIREKDEFLIPFIDTLTDPHFKTGTPDADQQLLKSLIVFSCLMEGLFFY
                     VGFAQILALGRQNKMTGAAEQYMYILRDESMHCNFGIDLVNQIKLENPQLWTVQFRDE
                     LQGLFRRAVELEYRYAEDTMPRGVLGLNAPMFKEYLRFIANRRCQQIGLETLYPGATN
                     PFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD"
BASE COUNT         2287 a         4849 c         4702 g         2201 t
ORIGIN      
        1 aattcgatgt tttcatggga gccgcaccgg cgttgtcttt gtcagtgggc tgtggaaaaa
       61 tcgtcggaga ttctaacttg aggcgtgcag cggtgctcga caaggaaggc taccggccga
      121 acgtcggcat catccttgcc aaccagagga acgaggtgtt ctggggcaag cgggtgaacc
      181 agcacgcctg gcaattcccg cagggcggca tccagggcgg cgagaccccg ctgcaggcca
      241 tgtaccgcga gctcgaggag gagatcggcc tgcaacgcgc gcacgtgcgc atcctcggcc
      301 gcacccgcga gtggctgcgc tacgaggtgc cggagcgctg ggcccgccgc cagcgcgagc
      361 ccaaggctgc cgcgtatcga gggcaaaagc agatctggtt cctgctgcgc atggtggggc
      421 gcgattgtga cgtgcgcctg cgcgccagcg gccatcccga gttcgacgcg tggcgctggc
      481 acgagtactg ggtgccgctc gacgcggtga tcgacttcaa gcgcgacgtc taccgccagg
      541 ctttgatcga gctgcaccgc tatctgctcc acgaccgtcg cgcgccccag cgcgccgaag
      601 ccctgatcag atgatcaggc gagacagcct gctctccctg gaagcgtatg cccgaggccg
      661 caacgagttt cgcgcgcggg tcatcgagca caagaagcgc cgcacgctgc acatcggcga
      721 gcacgtcacg ctgcagttcg aggacgagct gctgatccgc taccagatcc aggagatgct
      781 gcgcgtggag cgcatcttcg aggacgacgg gatccagggc gagctcgacg cctacaaccc
      841 gctgctgccc gacggcggca actggaaggc gaccatgttc atcgagtaca ccgacgtcga
      901 gcagcgcaag cgcatgctcg agcgcctgaa gggcgtcgag cggcgcacct gggtgcaggt
      961 cggcgacgcc gagcgcatct acgccatcgc cgacgaggac ctcgagcgcg agaacgagga
     1021 gaagaccgcg gcggtgcatt tcgtgcgctt cgagctgccc aaggccctgc gcgaggcgct
     1081 gcgccgcggc gcgcgcctga cgatcggcgt cgaccatccc gcctaccgcg cggtcctcga
     1141 gccctcgccc gagcagcgcg ccgcgctgct cgcggacctg gcttgaggcg gctgctcggc
     1201 gccgccgccc tggcgctggc ggcggcctgc ggcagcccgt cggtcgagaa gagcgactgg
     1261 gagcgcgaga acgaaacgcg cctgctgatc cccgaggaga ccccggcgcc gccggcgtat
     1321 cccgcgcgcg gccagctggt gcgcttcttc accctggagc gcagcgactt caacttcttc
     1381 gtcgacgctt cgtcgatcag catcggcaag gacggcgtgg tgcgctacgt gctcgtggcg
     1441 cgcagcccgg agggcgcgga caacgtgacc ttcgagggca tgcgctgcca gaagccggag
     1501 ctgaaggtct acgccgtggg gcgcgacggc ggctgggccg gcaggccggg cgcgtggcgt
     1561 gcgttcgaga ccggccgcac ccagccctgg cacctcgtgc tctaccgcga ctttttctgt
     1621 ccggggcgcg cgccgatctc gagccccgag aaggccgcgg ccgtcctgcg ccgcggcggc
     1681 gcgcgcggca tgaccgacta gagcagcacc agattgtcgc ggtggatgag ctcgggctcg
     1741 tccacgtagc cgaggatcgc ctcgatctcg cccgaaggcc ggcggatgat gcggcgcgct
     1801 tcctcggcgg aatagttcgc caggccgcgc gcgacttcgc gccccgcggg atcgagcacc
     1861 gtcaccactt cgccgcgctg gaattcgccg gtcacctcgg tgacgccgat cggcagcagg
     1921 ctcttgccgt cgcgcaccag ggcgcgcacc gcgcccgcgt cgaggcgcac ctggccggcg
     1981 acggtgagat ggtcggcgag ccactgcttg cgcgcggcga gcggcacggt ctgcgcggtg
     2041 agcagggtgc cgacgcgctc gccccgcgcg aggcgcagca ggacctcggg ctcggcgccc
     2101 gaggcgatca cggtgtgcgc gccgctgcgc gcggcgcggc gcgcggcgag caccttggtc
     2161 agcatgccgc ccttgccgat ggcgctgccg gcgccgccgg cgagcgcttc gagcgcggga
     2221 tcgctcgcat cgacttcttt cagcaatttg gcgttttcat tcttgcgcgg atcggcgtcg
     2281 aacagcccgg cctggtcggt gagaatgatg agtgcatcgg cttcgaccag gttggtcacc
     2341 atcgcgccga gggtgtcgtt gtcgccgagc ttgatctcgt cggtgaccac ggtgtcgttc
     2401 tcgttgatca ccgggatcac gcccaggccg agcagcgtgc gcagcgtcga gcgcgcattg
     2461 aggtagcgct gccggtcggc catgtcagca tgcgtcagca gcacctgcgc gctgtgcagg
     2521 ccgtggctgc ggaagcagct ttcgtagcac tgcgcgaggc ccatctgtcc gaccgcggcg
     2581 gcggcctgca gctcgtgcat cgcgtgcggc cgcttcgacc agcccaggcg ctgcatgccc
     2641 tcggcaatcg ccccgctcga caccagaatg acctttttcc ccaacgcatt gagctgggcg
     2701 acctggccgg cccagcggcc gatcgccgcc gcgtccagcc cgcgcccctc gttcgtcacg
     2761 agcgacgagc cgaccttcac caccagcgtt ttcgcctgct cgatcatttg ctcattttgc
     2821 gagctcgcgc gctgccgcct tggagaccgc ggcgcatcct tccccgctga gagcggagac
     2881 tttgaaccag gggcgcttcc agcgcaatgc acgcaggact ctttggatgc gagcgtccgc
     2941 gtcatcgacg atatcgatct tgttgaacac cagccagcgc ggcttgcgct cgagtccctg
     3001 gccgtacttt ttcaattctg cggcgatgat ccgcgccccg cccaccaagt cgggctcggt
     3061 gaggtcgacc aggtgaagca gcagccgcgt gcgggcgagg tgccggagga actggtggcc
     3121 gagccccgcg ccctcggaag cgccttcgat caggccggga atgtcggcca cgacgaagct
     3181 cgcctcgccc acacgcacca cgccgagcga gggatcgagc gtggtgaagg gatagtccgc
     3241 gaccttcggg cgggcgctgg acaccgcgcg gatcaagctc gacttgccgg cgttgggcag
     3301 cccgagcagg ccgacgtcgg cgagcacgcg caactcgagc gtgaggtcgc ggctctcgcc
     3361 cggctcgccg cgggtgaact ggcgcggcgc ccggttgacg ctcgacttga aatgcacatt
     3421 gcccaggccg ccctggccgc cgcgggcgag gagggcgcgc tcgccgtcgc gcgcgagatc
     3481 ggcgaccagc gcgccgtcgt cctcgtcgcg gatcagcgtg cccaccggca cgcgcagctc
     3541 gacgtcgcgc ccggcgcggc cgtcgcaatc cgatcccgtg ccgcgcacgc cgttctcggc
     3601 ccggtggatg cgcgcgaagc ggtagtcgac cagcgtgttg atgttgcggt cggcgcgcgc
     3661 ccacacgctg ccgccgcggc cgccgtcgcc gccgttcggc ccgccgtagc ggatgtactt
     3721 ctcgcggcga aaggtcgcgg cgccgtcgcc gcccttgccg gcgtgcacct cgatcttcgc
     3781 ttcgtcgatg aacttcatat cgcagaaata aaaaagccct accggcttcg atagggcttt
     3841 ccgttgagta ggtaccgttt agggtacggg agacgcgtct cccgcgccct agtttgccgg
     3901 cgatttttcg gcgatcacgc tcaccgtgtt gttctggtct gcgccgcgct gcgcgtactg
     3961 cacctgaccg ctcacgcgcg cgaacagcgt atggtcgcgc cccatgccga cgttcacgcc
     4021 ggcatgcacc tttgtgccgc gctggcgcac caggatctgc ccggcgttga cgcgctcgcc
     4081 gccgtagact ttgacgccga ggcgcttgga ctgggagtca cgtccgttcc tcgaacttcc
     4141 gccggctttc ttgtgtgcca tgcttaccct tggacgatgt cgtcgatgcg gacctcggtg
     4201 tagttctgcc gatggccctg ggatttctgg tagtgcttgc gccggcgcag cttgaagatc
     4261 ttcaccttct cgccgcggcc gtgcgcgacc acggtcgcct tgaccttcgc gccggagacg
     4321 agcggcgcgc cgaccttcac cgaatcgccc gtgccgacga gcaggacttc ttcgaatgcg
     4381 accgcggctc cgacgtcggc cgcaagcgtc tcgacgcgca cctgcttgcc ggactcgacg
     4441 cgatattgct tgccgcctga cttgatcact gcgtacattg ttgattccct gcctgaggtg
     4501 ttgcttccga aaagacctgg aattataagg taatcatgag tttgctgtcc acgctggcgc
     4561 cggtggccga cgacctcgcg cgggtcgacg ccctgatccg gcggcgcctc gactcggacg
     4621 tggcgctggt gcggcaggtg gccgagtacc tcgtggccgc ggggggcaaa cgcctgcgcc
     4681 cggcgctgct gctccttgcc tgcggtgcgc tgggatacaa ggaccggaaa agctccggta
     4741 acggcgggtc tgaagcccgc cttatgctcg cggcagtggt cgaattcatt cacaccgcca
     4801 ccctgctgca cgacgacgtg gtggatgcct ccgacctgcg ccgtggccgc accaccgcca
     4861 acgccgtgtt cggcaacgcc ccggcggttc tggtcggcga tttcctctac tcacgcgcgt
     4921 tccagatgat ggtcgaggtc gacgacatgc gcgtgatgca ggtgctcgcc gaggcgacca
     4981 acaccattgc ggcgggcgag gtgatgcagc tcatggggag ccacgacccc gaggtcgacg
     5041 aggcgcgcta cctcgaggta atccggcgca agacggcgaa gctgttcgag gccgcggcgc
     5101 gcctcgccgc cgtgcttgca agacaaggga attttgaaca accccttgcg acgtacggcc
     5161 ggcatgtcgg caccgcgttc cagctgatcg acgacgtgct cgactacggc ggcgagctcg
     5221 gcaagagcct cggcgacgat ctcgccgaag gcaagcccac gctgcccctc atctatacga
     5281 tgcgctgcgg cagcgcgcag caggccgcgg tagtgcgcag ggccatcgcc gagggcgggc
     5341 gggaggactt tgccagcgtg ctggaggcga tccgcgcctg cggcgcgctc gactatgcgc
     5401 gcgtcgcggc gcagcgcgaa gtcgatgccg cgacccgcgc catcggcgcc cttcccgagt
     5461 ctgagtacaa gcgatctttg ctagaattgg cggccttcgc ggccacccgc tcatccaacc
     5521 caatcggggc gtagctcagc ctggtagagt actgcgttcg ggacgcaggt gtcgctggtt
     5581 caaatccagt cgccccgacc acctttcccg tccttgcgtt ttttgcccgc gtagtttccc
     5641 gtagataatc gcgcgatggg ccggctgatc gtgctgatcc ttctggtcgt gctcgccgtg
     5701 tggctgatcc ggcgagccct gcgcgcgtcg agccgggccg agccggcgcg gaaggaaagt
     5761 cctgcacaac aagatctgat tcgttgcgcc cgctgcggcg tgcacctgcc gcgcgcggag
     5821 gctcgcctcg cgggcgggct cctgtattgc ggcgaggagc acgcccggct cggcgccggg
     5881 gagggctgat ggaagcctcg gtgcgcgccc cggtcacgct gtccgagcgg ttcgccccgc
     5941 ccgaggaggg cgttccggag tcgttctgga tctcgctgcg ctacttcaac ctgtaccgca
     6001 tcgcgctggc gggcctgttt ctcggcaccg cggtgatcta cggcgacgcg ctcaacctcg
     6061 gcacccacaa cctggcgctg ttccgctatt tcgccgcggg gtatctgctc cttggggtcg
     6121 tgttccacgg cgtgctgcgc gatcagcgcg accgcttcga gctgcagctc accctgcagg
     6181 ccggcctcga catcgtggcg atcacgctcc tcatgtacgc gagcggcggc attcgcagcg
     6241 gcctgggcgt gatgctgctc atctcgctca ccggcgcggc gatcgtcgcg ccgcgccggc
     6301 tcacgtttct ctacgccgcg ctcgccacga tcgccctgct gctcgagcag agctactggg
     6361 tgctcgagca cgacgccccc accgcgagct acgtgcagcc cgggctgctc gccatcggct
     6421 acttcgtcgc cgccggcgtc accagctggc tcgccgcgcg cgtggcggcg aacgagcgcc
     6481 tcgcgcgcca gcgcgggcgc gagctcgcgc ggcagaccca ggtcaaccag ctggtgatcg
     6541 aggacatgaa cgagggcgtg ctggtgctcg accgcggcgg caccgtggtg cagcacaatc
     6601 cccaggcgca ggagctcctc ggcgccgccg gcctgatcgg cgcagccatc gccagcgtgg
     6661 ttcccggctt ctcgggcagc ggcgagttcg aggtcgccgg gcgcgacatc cgcctgcgcc
     6721 cgctcgaagc gagcgccgag gagttctcgg tgtggctggt cgaggacacc acgcgcgcgc
     6781 gcgagcaggc gcggcagctc aagctcgccg ccctcggccg gctgacggcg aacatcgcgc
     6841 acgagatccg caacccgctc gccgccatca gccacgccac cgagctcctg cgcgaggaga
     6901 agcgcggcga cgatcgcgag cgcctgacgc gcatcatcca cgacaacgcc gggcggctcg
     6961 agcgcctggt gtcggacgtg ctgcagctga acaagcgcga ccgcgtctcg gccgcgccgc
     7021 tgcgcctgca ccagtggctc ggcgccttca tcacggagtt cgtcgccaac gaggcggtgg
     7081 cgcaagggcg catcgtgctc gagacgcggc gcgagtcgtg gatcgagttc gaccacgagc
     7141 acctgcacca ggtgatgtgg aacctgctgc gcaacgccgt gcgctacgcg cccgccgagc
     7201 cgggcagcat ccgcatccgg ctcgacggct atgccgaccg cgtcgagctc tcggtgatcg
     7261 acagcggccc cggcgtgccg gcggcgatcc aggggcagct gttcgagccc ttcttcacca
     7321 ccgaggcgaa gggcaccggc ctcgggctgt atctggcgcg cgagctgtgc gcggcgaacc
     7381 gcgccgtcct cgaatacgtg aacggcgggg ccggcgggca tttccgcatc cgctgccgcc
     7441 aggcggcgtc caaatgagcg tgtccgcagg taggggtaga gtgcccgcat gacccgccgc
     7501 ccggaacggc gcgcgacgca gcgcgacgcc aaggtgctcg tggtcgacga cgagcccgat
     7561 atccgcgagc tgctggagct cacgctggcg cgcatgggcc tcggcgtcga cgcggtgggc
     7621 tcgatcgccg aggcgaagga gcgcctgaaa accgagcgct acgacctgtg ccttaccgac
     7681 atgcggctcg gcgacggcga ggggctcgag ctcgtgcgcc acatcaccac gctcgcggcg
     7741 gacctgccgg tcgccgtgat caccgcgtat gggagcgccg acaacgccgt ggcggcgctg
     7801 aaggcgggag cgttcgacta cgtgtcgaag ccggtcgggc tggagcagct gcgcgcgctg
     7861 gtgaaatcgg ccctctcgct gcccgagcgc gccgagggcg cgggggacac cgcgggccgc
     7921 cagctcctcg gcgagagcgc gccgctcgcg caggtgcgcg agctgatcgg caagctcgcc
     7981 cgcacccagg ccccggtgta catctcgggc gagtcgggca gcggcaagga gctcgcggcg
     8041 cgcctgatcc acgaaaacgg cgcacgccga gagaagccgt tcgtcgcggt gaattgcggc
     8101 gccattcccg acaccctggt ggagagcgag ctcttcggct accgtaaggg cgcgttcacc
     8161 ggcgccaacg aggaccgcga cggcttcttc caggccgcgc acaacgggac gctgtttctc
     8221 gacgagatcg ccgagctgcc gctgcatacc caggtgctgc tgctgcgcgc aatccaggag
     8281 cggcgcgtgc gcaaggtggg ctcgacgcag gaggagccgg tggacgtccg catcatctgc
     8341 gccaccaacc agagcctcgc cgggatggtg gaggcggggc gcttccgccg cgacctgtac
     8401 taccggctga acgtggtgga cctcaccatg cccccgttgc gcgaatgccg ggaggacatc
     8461 ccgctcattg ccacccacat cctgcaacgc ctgtcggccc agaacggcgt cccggcgccg
     8521 agcctttccg cgccggcgct ggaagagctg atggcctacg acttccccgg caacgtgcgc
     8581 gagctcgaga acatcctcga gcgcgcgctc gccctttccc caggccagga gctcggcccg
     8641 gcggaccttg ccctgcaccg gatcgccccc gaagaagagg acgccagccc ccccggggaa
     8701 gggcagcccg cccggccggc cgactccccg ctgccggcct acctcgacca actcgagcgc
     8761 gaagcgatcc tgcaggcgct cggcaagacc ggcttcaacc gtaccgccgc tgcgcgtttg
     8821 ttagggatca ctttcaggca gctgcgctac cggatgcagc gcctcaacat caaagatgag
     8881 aatcgttgat tcgccgaact gcgacgagcg tcccgcgggg gttgaagtca gcttggtcgt
     8941 tcttcatagc atcagcctgc cgcccgggga gtacggcggc gactcgatcg agcgcctgtt
     9001 caccaactgc ctcgacccgc aggcgcatcc ctacttccag gaaatccgcg gggtaaaggt
     9061 ctcgtcgcat ttcctgatcc ggcgcgacgg cgaggtcgtg cagttcgtgc cggtcgagcg
     9121 gcgcgcctgg cacgccggcg cttccgcctg gcgcggccgc agccgctgca acgacttctc
     9181 catcggcatc gagctcgagg gcaccgagga cgatgccttc accgatgcgc agtaccgggc
     9241 cctcacctcc ctcttgaaga aactgcgcac ccggctgccg ctgcgcgacg tcgccgcgca
     9301 cagcgacgtg gcgccggggc gcaagaccga tcccggcgcg cgcttcgact gggcgcgcct
     9361 gctcgccggc ctggcgaagt cgcgcgccgc tcccgtttcg caacactaga tagccgctcg
     9421 ctgcatcgct ttggcgcaag atgtagtgcc cctgcgcgcg atccctagat ttagtgcgac
     9481 aacgtcgtgc gaactactac atccggtgcc tggttgcgcc gcagcatcgg cgacgccgct
     9541 tgcaaattcg caaacccctc ggtagattgc gttccgtctg gtccaaaaga actagatata
     9601 ggccaagtag ggccgtcgaa cactaaacgt agtagcagca agggtgggcg tcagggttga
     9661 cggcccggtg acacaaaaaa ggggcatggg aggcggtatg cagatcgctc aagacagcgc
     9721 cacggcaact tcgtctctga gcgtcggcat tgccgagtcg aatacgccgg cgcgcggcgt
     9781 ctacgccgac tacaaggtga tccgccgcaa cggcgcggtg gtcggcttcg agccggccaa
     9841 gatctcggtg gccatgacca aggcgttcct cgcggtcaac ggcgcgcagg gcgcagcctc
     9901 ggcgcgcatc cgcgagctgg tcgccggcct gaccgacaac gtggtcggcg cgctgctgcg
     9961 ccgccagccg cagggcggga ccttccatat cgaggacatc caggaccagg tcgagctcgc
    10021 gctgatgcgc tccggcgagc acgacgtggc gcgcgcctac gtgctgtacc gggaagagcg
    10081 cgccaaggcc cgctcgcagg agaagacttc caggaaagat gaaattcacg tcgtcgagag
    10141 cggcggccgc aagccgctcg accgcgcgcg gctgcagcgc ctggtggagg cggcgtgcga
    10201 aggcctcgcc gacgccgcgc cgcaggcgat cctggaggcg acgctgcgcg acctgtacga
    10261 cggcgtgccg atgcaggaag tgcgcaagtc cgcggtcctc gccgcgcgcg ccctgatcga
    10321 gaaggacccg gcgtacagct acgtcacggc gcggcttctc ctcgacacga tccggctcga
    10381 ggtgctcggc gaggaagcga gccaggccga gatggaggcg cgctacgccg agtacttccc
    10441 gcagttcatc gcccgcggca tcgccgccga gctgctcgac ccggagctcg ccaagttcga
    10501 cctggaccgg ctgggaaagg ccctgaaggc caagcgcgat ctgcagttcg gctacctcgg
    10561 cctgcagacg ctctacgacc gctacttcct ccactcgggc ggccggcgca tcgagctgcc
    10621 gcaggcgttc ttcatgcgcg tcgcgatggg gctcgcgctg aacgagaaag accgggaggc
    10681 gcgggcgatc gagttctacg gcgtgctgtc cgcgttcgac ttcatgagct cgacgcccac
    10741 gctgttcaat tccgccacgc tgcgctcgca gctctcgagc tgctacctca ccacggtgtc
    10801 ggacgacctc accgggatct acgacgcgat caaggagaac gcgctgctcg ccaagtacgc
    10861 cggcggcctg ggcaacgact ggacgccggt gcgcgcgctc ggctcgcaca tcaagggcac
    10921 caacggcaaa tcgcagggcg tggtgccgtt cctcaaggtg gtgaacgaca ccgcggtggc
    10981 ggtgaaccag ggcggcaaga ggaagggcgc ggtgtgctcc tacctcgagg tctggcacct
    11041 cgacatcgag gagttcctcg agctgcgcaa gaacaccggc gacgaccggc gccgcaccca
    11101 cgacatgaac accgcgaact ggatccccga cctgttcatg aagcgggtga tggaaggcgc
    11161 ggagtggacg ctcttcagcc cctcggacgt gcgcgacctg cacgacaagt tcggcaaggg
    11221 cttcgaggaa gcgtacctgc gctacgagga caaggcgtcg cgcggcgagc tcaagctcta
    11281 caagcggatc cccgcgctgc agctctggcg caagatgctc tcgatgctgt tcgagaccgg
    11341 gcatccgtgg atcaccttca aggacccgtg caacctgagg agcccgcagc agcacgccgg
    11401 ggtcgtgcac agctcgaacc tgtgcaccga gatcacgctc aacacctcgg ccgacgagat
    11461 cgcggtgtgc aacctgggct cgatcaacct ggtcgcgcac ctgaccgaga ccggcttgga
    11521 tttctcaaaa ataaaaacaa caatttccac ggcgatgcgg atgctggaca acgtcatcga
    11581 catcaactac tacgctgtcg acaaggcgag gaactccaat ctgcgtcacc gtccggtggg
    11641 cctgggaatc atgggcttcc aggactgcct gcaccgcctg cgcctggcgt atgcctcgcc
    11701 cgaggccgtc gagttcgccg accgctcgat ggaaatggtg gcgtacgcgg cgtactgggc
    11761 gtcgaccgag cttgccgcgg agcgcgggcg ctacgcctcg ttccacggct cgctgtggga
    11821 ccgcggcatc ctgccgcagg acacgctcga catcctgtcg gcggagcgcg gcggctacgt
    11881 cgagctcgac cgctcctcga ccctcgactg ggaggcgctg cgcgggcgca tcaagcagca
    11941 cggcatgcgc aactccaact gcctcgcgat cgcgccgacc gcgaccatcg cgaacatcgt
    12001 gggggtgtcg gcctcgatcg agccgaccta ccagaacctg ttcgtcaagt cgaacctctc
    12061 gggcgagttc accgtggtga acgactacct ggtggccgac ctgaagcgcg ccgggctgtg
    12121 ggacgaggtg atgatcgccg acctcaagta cttcgacggc aacctggcgc gcatcgaccg
    12181 ggcccccgcc cagctgcgcc ggctctacgc caccgcgttc gaggtcgagc cgaagtggct
    12241 ggtcgagtgc gcggcgcggc gccagaagtg gatcgaccag tcgcagtcgc tcaacatcta
    12301 catggcgggc gcctcgggca agcggctcga cgagacctac aagctcgcct ggatccgcgg
    12361 gctcaagacc acgtactacc tgcgggcgct cggcgccacc cacgcggaga agtcgacttc
    12421 gaaggccggc gagctgaacg cggttccgat ggaaaacccc gagccggcca agatgtgcgc
    12481 catcgacaag cccgactgtg aggcgtgtca gtgattactc tcggcagccg cagcttcgcc
    12541 gggccattca tggcgccgct gtggtcgccg ccgaagggcg ccggcctgta cgccgtgatg
    12601 gcgccgggct ggcggctgtt gaccttccgt gccctggatt tcgagcgggc gggagatctg
    12661 gccgagcccc aactgctgaa aaagcacccc aaataccgcg agtggatcgg catcgtcggc
    12721 accgagtgga acctgtacat cgcgacctgc gagatggcgg tgtccaccga ggctgagcgc
    12781 gaggccgcct tgcgcacgct gctgcgcgag taccggccgg aattcaagcc gctcagtcca
    12841 gaccccgtcc cgccccgagg acaactgaaa cccacatgct gaatttcgaa gacctgacca
    12901 gcgcgcccat ccccggcgca accccggtga ggcgcgaggc gcccccggct ctggacgccg
    12961 cgctgcggcg cgtgaccgtc gacgacaaga agatcatcaa ctccaaggcc gacgtcaacc
    13021 agctggtgcc cttcaaatac aagtgggcct gggagaagta catcgcggcc tgcgccaacc
    13081 actggatgcc gcaggaaatc aacatgagcc gcgacatcga gctgtggaag gacccgaacg
    13141 ggctcaccga ggatgagcgc cgggtcatca agcgcaacct cggcttcttc gtcaccgcgg
    13201 actcgctcgc ggccaacaac atcgtgctcg gcacctaccg ccacatcacc gccccggagt
    13261 gccgccagta cctgctgcgc caggccttcg aagaggcgat ccatacccac gcctaccagt
    13321 acatcgtcga gagcctggac ctcgacgagt ccgagatctt caacgcctac cacgaggtca
    13381 cgtcgatccg ggagaaggac gagttcctca tccccttcat cgacaccctg accgacccgc
    13441 acttcaagac cggcaccccg gacgccgacc agcagctctt gaagagcctg atcgtgttct
    13501 cctgcctgat ggagggcctg ttcttctacg tcggcttcgc ccagatcctg gcgctcggcc
    13561 gtcagaacaa gatgaccggg gcggccgagc agtacatgta catcctgcgc gacgagtcga
    13621 tgcactgcaa tttcggcatc gacctcgtca accagatcaa gctcgagaac ccgcagctgt
    13681 ggacggtgca gttccgcgac gagctgcagg ggctgttccg ccgcgcggtg gagctcgagt
    13741 accgctatgc cgaggacacc atgccgcgag gggtgctcgg cctgaatgcc ccgatgttca
    13801 aggaatacct gaggttcatc gccaaccgcc gctgccaaca gatcggcctg gagacgctct
    13861 acccgggagc gaccaatccg tttccctgga tgagcgagat gatcgacctc aagaaggagc
    13921 gcaatttctt cgagacccgc gtgatcgagt accagaccgg cggggcactg agctgggatt
    13981 aacagggggg acaacgtgct tgaggaattc gacaccatgc gaataacggc gtgcaaggc
//
LOCUS       DATBSZ010000292        48342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_263931, whole genome shotgun sequence.
ACCESSION   DATBSZ010000292 DATBSZ010000000
VERSION     DATBSZ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 48342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48342
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263931"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VIG70_19025"
     CDS             complement(<1..310)
                     /locus_tag="VIG70_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8252687.1"
                     /translation="MVIEQIIGLYQAKGGREYEGEGITQLEHALQSAHLAEQAGAAPA
                     LVCAALLHDIGHLLNDRGETPTLRGIDDLHQYVALPFLRPHFPEAVLGPIRLHVDAKR
                     Y"
     gene            complement(304..1752)
                     /gene="phnY"
                     /locus_tag="VIG70_19030"
     CDS             complement(304..1752)
                     /gene="phnY"
                     /locus_tag="VIG70_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HEY8252688.1"
                     /translation="MHARTRTVESPRREALRIAGEKVYRDRTIEVRYPYTGEVVATIA
                     KATVDDVKRAFRVARDYKPKLTRYERYKILMRAGEIIASRRDEIARGITLESGLCIKD
                     STYEVGRASDVLLFAANQALVDDGQTFSCDLTHHGKSRRVYTMREPLMGVISAITPFN
                     HPLNQVIHKVAPSVATNNRMVLRPTNKTPLAALVLADVLYEAGLPPEMLQVVTGDPKE
                     IADEMLTNPDVDMVTFTGGVPIGKYIAAKAVYKRQVLELGGNDPLVIMDDADPAKAAD
                     LAANGSYKNSGQRCTAVKRILVHEKIAEEFVSRLVEKTKAWSYGDPLDPKVDMGTVIS
                     EQAASSFEGLVNEAVSKGARLLCGNARQGALYSPTVLDRVQPEMPVVKYETFGPVSPV
                     MTFKSVDEAIRLVNATDFGLSSGVCTNRLDDIMRFVKELNVGSVNVWEVPGYRLELTP
                     FGGIKDSGLGYKEGVQEACKSFTNVKTYSLPW"
     gene            complement(1767..2810)
                     /locus_tag="VIG70_19035"
     CDS             complement(1767..2810)
                     /locus_tag="VIG70_19035"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY8252689.1"
                     /translation="MASSGKLAVYDAPGKPFELRSYPLRPPRADEALVKVRMSTICRS
                     DIHSYLGHRPNPCPGVLGHEIIGNLVEMGSDIKRDMRGDSLAVGDRITWSEYFVPPGS
                     ETLELPQKAPGVDKYGHMAVTTDPHHHGGFGEYCYILPRSWILKLPPELTDEEATPIN
                     CGVATVVCIVEKAEVAKGDTVVIQGLGLLGLYAAAVCKARGARRVIGVDTVAARREMS
                     ARFGVDQAVADLKGVEGVDVVIEVCGNPEVIPAGIQALRIGGRYALGGVVNPDAFVCI
                     DANQILRKLVTLRGVHNYHPRNLPEALDFVVANRKRFPFHDLVDGKYRLDEVGKAMQD
                     AADRRVLRAAIVP"
     gene            complement(2818..4065)
                     /gene="phnA"
                     /locus_tag="VIG70_19040"
     CDS             complement(2818..4065)
                     /gene="phnA"
                     /locus_tag="VIG70_19040"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HEY8252690.1"
                     /translation="MKDTVTVNGTRYRWPKKPVAVVCIDGGDPAYLQQFLRDGSIPNI
                     ARFIRQGFSTVADGTVPSFTCPNNMSIITGTPASRHGISGNFYLDVSTGQAVVMTGPE
                     LLRGDTILSQFARAGAKVVSITAKDKLRKQLGKNLDVSKGNISFSSEFASKATLKENG
                     IENVLALVGMPQPGMYSMELSLFVLEAGIKLLERDRPDLLYLSLTDYVQHKYAPEESE
                     AKRFYQALDRCFGRLEALGAVVALTADHGMNDKSDAKGKPNVIWLQDMLDAKFGKGDT
                     RVICPITDAFVAHHGALGGFVRVWCTGKARPKQVIDAIHGLEGIESVMDKETACRVFE
                     LPADREADVVVISTANVCIGGSSAAHDLSGLEGHRLRTHGGVSEAKVPFILSVPLNDD
                     YKLKAGVSTLKSYQIFEYALNGW"
     gene            complement(4062..5222)
                     /locus_tag="VIG70_19045"
     CDS             complement(4062..5222)
                     /locus_tag="VIG70_19045"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY8252691.1"
                     /translation="MSKRSAKLNTQARAAASRRKFLGAATAATTGAAMLGFPMIARAQ
                     APQIRWKLQSANPAGTPHMELLSRLVANIDSMSGGRLKIEVLPSGAIVAPFEILDGVN
                     KGIVEAGQWWTHYATGKHPAGGLFSAPIGGAGSGLDQMNHLSWYLRGGGRELYLEYYQ
                     KILKADVQAFLYAPDGPEAFGWVKRVPKTVAEYTKMRFRMSSGLPSDVLKDMGGVPMN
                     LAGAEVIPAAERGLIDGVEWINPANDLKLGLSDVFKNYSLQGLHQAIDIADVVISGRK
                     WRELTPDLQAIVQTAMTTSIFEAILYFVDENSKALQVLTRDKGVKMFDAPSGYASAFI
                     KSAKKVLAQYEAKDAFFKRVLESQRKFASTVVPYTKETNKLTQLIAGAAEGS"
     gene            complement(5259..6575)
                     /locus_tag="VIG70_19050"
     CDS             complement(5259..6575)
                     /locus_tag="VIG70_19050"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY8252692.1"
                     /translation="MSLEIYGLILLVLLFGVICLGFPISFTLITLGMVFGYIALGERV
                     FHLMTLSYFGTMTDTVLAAVALFTFMGYVLESAGLMERLFRAVQLISGRIRGSLYMAT
                     IFTATLFAAATGIVGASVTIIGLMAAPVMKKAGYNTGLSAGTICAGGTLGILIPPSVM
                     LVVMGPVFSVSVSRLYAAAIIPGLLLAFIYLSYTMTRVMLNPSLGPALSDEELKVPTS
                     FLVREFFVGLIPPVTLILVTLGSIISGLATPTEGAALGCLGALGVTASHGRLTFKIIK
                     ESAFRTAETTSMVMILLAASTFMGVVFSAMGTPKFIAETMLAWDFPPAVFLLGLLVVC
                     FILGWPLEWVPIVVVIVPIFLPVLTQLKTDMIWFSILLAVTLQTCWLSPPVALSAYYL
                     KGIMPEWELLEIYKGMMPFMFLQAIAVALLYIFPQIVMWLPNLLFG"
     gene            complement(6597..7154)
                     /locus_tag="VIG70_19055"
     CDS             complement(6597..7154)
                     /locus_tag="VIG70_19055"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEY8252693.1"
                     /translation="MATRTALRAMDRVIAVIDQVSIWSGKAIAWLIFPMIAMLGGEVI
                     VRYAYAPSIWALDVATMLYGTHYLMAAAMTLYLGKHIRTDFFYGNWAPRTQAWVDVIC
                     YLLLFLPGMVMFVWLSWDFAAESWDLGERLMTTWRPPAYWFKSVIPLSAALLLLQGIS
                     ELLKSVKLLLTGVDIRHREAPGEIT"
     gene            complement(7154..7846)
                     /locus_tag="VIG70_19060"
     CDS             complement(7154..7846)
                     /locus_tag="VIG70_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate utilization associated
                     transcriptional regulator"
                     /protein_id="HEY8252694.1"
                     /translation="MARQSRVALSPIELLRQESLTSLVQREIERRIVAGELVPGAKLN
                     EAELAAAMGISRGPVREAFRALEQAGLVHTEKNRGVFVRQVSLEEANEIYEVRAALEA
                     QIGKLAAKRATAPQLERLRAIVKRMQAVGRKRDPDAYFPLNIEFHEALAEAAGNRALA
                     ANYRRVVNELNLYRREAIQRNVDIIPVSTKEHAAIVDAVARGDARLAERLLYEHVINS
                     RSRLHDFKRAAA"
     gene            complement(7860..10265)
                     /locus_tag="VIG70_19065"
     CDS             complement(7860..10265)
                     /locus_tag="VIG70_19065"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8252695.1"
                     /translation="MVRQDADVVIIGGGAIGCSIAYHLSRFSKKSILLLEKAQLTHGS
                     TWHAAGLVGQLRSKRNLTRLMQNSVALYRTLERETGQAIDWHEVGSVRLASSAERWSE
                     MKRAATTARGFGFEMELISPKEAQAKFPYITLDGVAGAAWVPSDGYVDPSSLAQALAK
                     GARSAGVKILQDTRVTGFESKDRRITQVLTDKGAIDCEIVVIAAGIWSRAVGALMDVR
                     IPAAALEHQYMVTEPMKERNPKLPTLRDPDRNFYLKPEVGGFAVGGWEMGTPSFHAAG
                     VPFEFAQELLPPNMDRFEEIALATAERIPAFGELGLKQLINGPIPVSPDGEPIMGQAV
                     ERDNVFVACGFTSGIAAAGGAGRAMAEWIVQGYPEMDLWPFDLRRFGDFHSGTRALHE
                     RAVHAYGSYYHLHYPGEELTVARDARRSPLWQTLKERGAVYGSKFGWERPNWFSSPGM
                     QDKPSFEGKPNWFEHVAAEHRAVRERVALFDFSSFSKFEITGRGAFAALQRIVANDLE
                     KPAGSVVYTQLCNEKGGIEADLTVVRLSAEHFYIVTGSGFAVRDGQWISRQLPAEVQF
                     KEVTSSRAVINVQGPQSRELLSRVAEGDLSNAAFPYLAAREIRVGFAPVLALRLTYAG
                     ELGWELHVPSEYALHVYEALWDAGQDIGVANAGYRAVDSLRLEKGYRYWSSELSPDAN
                     PYETGLGFAVALDKGDFIGRAALQKIRKEGTRRKLACFLMDGFVPLHGGEAILHEGRV
                     IETVTSGGFGHTIGKSIAYGFVPMELAKATGFEIESFGVRHAARLSERVPYDPERRKI
                     VNC"
     gene            complement(10238..11476)
                     /locus_tag="VIG70_19070"
     CDS             complement(10238..11476)
                     /locus_tag="VIG70_19070"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY8252696.1"
                     /translation="MKQGLQGELAQHLAAYRPGWSLPRPFHRDEAVYRADIERIWRKG
                     WLFAGHGCEIRSPGDWLTLQVDTDSIIVIRGEDGKVRALHNVCRHRGSQICAPGRGSS
                     RRLVCPYHQWTYDTTGALVFCRGMHDFDRKGFSLKQVSCETVEGLIFISLAQAPPPFA
                     QARELMAPYLRPQGFERAKVAKQVDYEIAANWKLVWENNRECFHCNVNHPQYIKANWD
                     HYNSDDTTTEVRAAIDRVVKKSETKWAAAGLAVSHRDTGMTQFPDAERGIWYSANRTA
                     LVEGWVSESMDGAQVSTLMGEYRDPDVGTVRARTLPNFWNHSSCDHGVSTLLLPAGPR
                     KTLARVTWLVHEDAVEGRDYQLGKLMPFWQLTSEQDWAICERQQKGVDSTAYEPGPYS
                     KHKEYNVDAFVQWYVKTLTS"
     gene            11546..12568
                     /locus_tag="VIG70_19075"
     CDS             11546..12568
                     /locus_tag="VIG70_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8252697.1"
                     /translation="MLKYLLQLAALTALALSASLALAQRTPILVYTAIETDQLKAYKD
                     AFEAAVKDVEIRWVRDSTGVVTAKLLAEKANPQADVVLGLAATSLLVLEQEGMLTPYA
                     PQGVEKLNQRFVDAKKPPMWVGMDVWGATICFNTVEAKKRNLPKPESWKDLLKPVYKG
                     QITMPHPASSGTGFLDVTAWLQLFGEAEGWKYMDALHENIAQYVHSGSKPCRQAGAGE
                     FVMGISFEYRGNATKASGAPVDLIFPKEGVGWDMEASAIMKASKKQAAARKLLDWSVT
                     RGANELYAKNYAIVALPGVAKPLPNLPSNYESMLVKNDFAWAAKNRDRILAEWEKRYN
                     AKAEPK"
     gene            12543..13640
                     /locus_tag="VIG70_19080"
     CDS             12543..13640
                     /locus_tag="VIG70_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY8252698.1"
                     /translation="MTQKPNRSDATALAVEGVSKHFGGFAALSDVSLAVGQGEFVCLL
                     GPSGCGKTTLLRIIAGLETQSVGRIVQNGRDISVLPPAQRDYGIVFQSYALFPNLTVA
                     DNVGYGLVNRRMARAEVRLRVAELLKLVGLPGSEPKYPSQLSGGQQQRVALARALATS
                     PGLLLLDEPLSALDAIERVRLRGEVRGLQKKVGVTTIMVTHDQEEALSMADRVVVMNK
                     GALEQVGTPREIYEQPATPFVADFVGRVNVLSAVSLGAGRYQAGRLALSAVNGATPHA
                     AGTRVRLYLRPEDRVVEGNLDSESNQMHGQIAKVEFLGGHCLAEIQVPELEGQALSVF
                     FSLNQVRELGIREGAGLRLALRPDRIRVFAS"
     gene            13648..15357
                     /locus_tag="VIG70_19085"
     CDS             13648..15357
                     /locus_tag="VIG70_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HEY8252699.1"
                     /translation="MSAVLAAERPPLLGVEFIARALLVLTGLGLALFLLAPMAAIFVR
                     AVQDSNGNYVGLVHFANYFATPSLARAVWNSVWVSLAVAAITVPAAFMFAYALTRSCM
                     PFKGTLRLIALIPLLAPSLLPAISLIQWFGNQGALKGPMQALGISSIYGPPGIILAEV
                     FNCFPHALMILIAALALADGRLYEAADALGTRRLRRFFTITLPGCKYGLLSAFTVVFT
                     YTISDFGAPKVIGGNFNVLAVDVFKQVIGQFNFSIGAVVGLLLLVPSVVAFGVDWMVR
                     RRLTANLTARSVPFIPKPRPLFDNLMLGYSALVCLLLLAVLGMAVYTSLIHLWPYNLG
                     FTLKHYHYALVGGDYAPAFWNSLKMGAWTAVIGTVFVFCTAYLLEKTRGLGPLRPVLR
                     LLALLSMAVPGLVLGLGYIMFFNHPQNPINFLYQTMAILAISTVVHYYTSGHLTAVTA
                     LKALDDEYESVSASLKVPFYKTFWRVTVPVCLPAILEIARYYFVVSISTLSCVVFLYA
                     PHTILAAVAIMNMDEAGDVGPACALGTLVVLASVGVCLLYALATRVLLAKSQAWRFNL
                     EKA"
     gene            15354..16484
                     /locus_tag="VIG70_19090"
     CDS             15354..16484
                     /locus_tag="VIG70_19090"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEY8252700.1"
                     /translation="MKEPILLTPGPLTTSTATKLAMVRDWGSRDTGFIEINRRVRAML
                     LDVVGLKESHVCVPLQGSGTFAVEAMLGTLVPKDGHVLVPLNGAYCKRIQRICGILGR
                     KATVLEFEEREPVKPAAVDAALARDPSITHVALVHCETSTGVLNPLAEIAAVVARHKK
                     SLLVDAMSSFAAVPIEGACDALVAASGKCLEGPPGMGFALIRKTVLEKCAGNAHSLVL
                     DLHDQWVNMEKTAQWRFTPPTVIVAALHAALEQFAAEGGRAARGARYRRNCEVLIEGM
                     TRLGFKLFLDPKHQAPVIVTFHAPSDPNYDFQKFYDRVREKGFVLYPGKLTQVDTLRV
                     GCIGAIDEHVIRAAVHAIADTVAEQNIRLHETGAVRSRQHAS"
     gene            16444..17100
                     /locus_tag="VIG70_19095"
     CDS             16444..17100
                     /locus_tag="VIG70_19095"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8252701.1"
                     /translation="MKLALFDLDNTLLSGDTDVEWLDFLVERGVLPASERAANHEMDR
                     RYRSGEAAALEYVRFYLRFYPAHDMSTLLGLRQRFVQARIEPRMLPAARELIRSHGKD
                     LIAIITATNRFLTEPIAAAFGVEHLIATEPQIEHGRFTGDVVGAPCMREGKIEHLERW
                     LAARDKVLGNFEESWFYSDSINDLPLLERVTHPVAVDPDISLEKIARDRGWRRLTLRG
                     "
     gene            complement(17264..18094)
                     /locus_tag="VIG70_19100"
     CDS             complement(17264..18094)
                     /locus_tag="VIG70_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HEY8252702.1"
                     /translation="MADAPEYEPEEVVDPDVDPRVDPRADPRAGARVGLRRRGIYLLP
                     NLFTTGVLFCGFFAIVQAMNGRFDIGCIAIFIAMVLDGMDGRVARWTNTQSEFGAQYD
                     SIADMVAFGAAPALMAYIWVLKDLGTAGWIGAFIYCAGTGIRLARFNANIAVVDKRYF
                     QGLPSPSGAAVMVGFVWALVDFGFVPREHAWLAPLTWGVAVFAGVTMVSNVPYWSFKE
                     VNWRKRVPLWVILACVVALSIIASSPSLVLFALVMGYAVSGYVMWAMGHRARPVLPEE
                     "
     gene            complement(18058..18729)
                     /locus_tag="VIG70_19105"
     CDS             complement(18058..18729)
                     /locus_tag="VIG70_19105"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEY8252703.1"
                     /translation="MSRRYPHPFIAREGWVFVLIALGISLLIGVFLGWWWSIPFWLAG
                     IFILQFFRDPPRSIPDDPRAVLSAADGRVVEVSRAQDPYVKRDALKVSVFMNVFNVHS
                     NRSPVDGTVKERWYFPGAFVNAALDKASLENERNALWLRTRDGHDVTCVQVAGLIARR
                     ILCYVGAGAELERGQRFGFIRFGSRVDVYLPLDAEVKAAIGDKVYAAESVLAWLTPPN
                     TSRRK"
     gene            complement(18734..19750)
                     /gene="ilvC"
                     /locus_tag="VIG70_19110"
     CDS             complement(18734..19750)
                     /gene="ilvC"
                     /locus_tag="VIG70_19110"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY8252704.1"
                     /translation="MKVYYDKDADLSLVKGKKVTIIGYGSQGHAHAQNLQESGVKVTV
                     ALRKGGASWDKAKKGGVKVAELAPAIKGADIVMVLLPDEHHAAVYQEYIEPNIKKGAS
                     LAFAHGFNIHYGLIQPRADLDVWMVAPKAPGHTVRSTFAAGGGVPMLIAVHRDPSKKA
                     RDVALSYAAAIGGGKAGIIETNFKEETETDLFGEQTVLCGGCVELVKAGFDTLVDAGY
                     APEMAYFECLHELKLIVDLMYEGGIGTMNYSISNNAEYGEYTSGPKIIDEGTRKRMKE
                     VLGRIQSGEYAKEFVLENRAGAPTLLSRRRMTAELPIEKVGARLREMMPWIKRNRLVD
                     TSKN"
     gene            complement(19770..20264)
                     /gene="ilvN"
                     /locus_tag="VIG70_19115"
     CDS             complement(19770..20264)
                     /gene="ilvN"
                     /locus_tag="VIG70_19115"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY8252705.1"
                     /translation="MSRHIISVLVENEAGALSRISGLFSARGYNIETLTVAPTEDASM
                     SRMTIVTTGSEDVVEQITKQLNKLIEVVKVIDLSEAEHIERELMLIKVRAGLKEREDM
                     KRMADIFRGRIIDVSDNTYTIELTGDGAKLDAFIRSLDQGAIIETVRTGASGIGRGNR
                     VLRV"
     gene            complement(20261..21973)
                     /locus_tag="VIG70_19120"
     CDS             complement(20261..21973)
                     /locus_tag="VIG70_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HEY8252706.1"
                     /translation="MDRAKTTTVTGAEIVVRCLQEEGVEYVFGYPGGAVLFIYDELFK
                     QDKVKHVLVRHEQGAVHAADGYSRSSKKVGVCLVTSGPGVTNAVTGIATAYMDSIPLV
                     VLTGQVPTHAIGLDAFQECDTVGITRPCVKHNFLVKNVKDLASTIKKAFHIAATGRPG
                     PVLVDIPKDVTQHACEFQYPESVQMRSYNPVRKGHAGQIKKAVQLLLEAERPMVYTGG
                     GVVLSDASLLLRELVRNLGFPCTNTLMGLGAYPATDKQFLGMLGMHGTYEANMAMQHC
                     DVLLAIGARFDDRVIGNPAHFAQNPRKIIHIDIDPSSISKRVKVDVPIVGNIPDVLED
                     LLKLLKGTAPRDIAPWWKQINEWRAKDCLRYDRKSAIIKPQFVLEKLYEVTKGEAFVT
                     SDVGQHQMWAAQFYKFNEPRRWINSGGLGTMGFGLPAAMGVQLANPGATVACVTGESS
                     IQMCLQELSTCKQYRLPIKIVNLNNKYMGMVRQWQQFFHGNRYSESYMDALPDFVKLA
                     EGYGHRGVLIEKPSDVEGALREAFAAKNDLVFLDFLTDQKENVYPMIPGGKGITEMIL
                     YEDL"
     gene            22083..22652
                     /locus_tag="VIG70_19125"
     CDS             22083..22652
                     /locus_tag="VIG70_19125"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8252707.1"
                     /translation="MASRSELSAFLEGVEKRAFKQAVFAVRDEKAALDIVQDAMLRLA
                     EKYGHLPAGELPALFHRIVQNAIRDWYRRQKVRSTWTTQLSSFGEDDEQDPLETLQPA
                     EGSEVQEGPAGQLEQAQVLEIIRQEINRLPERQREAFILRYWEELDVAATARAMSCSE
                     GSVKTHCSRAAHALQVALRARGITLDIKT"
     gene            22649..23017
                     /locus_tag="VIG70_19130"
     CDS             22649..23017
                     /locus_tag="VIG70_19130"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HEY8252708.1"
                     /translation="MNEQQLRQLLNQGITVRPEIAARLEQARKQALALQRPVPAPALA
                     WAGNAVGHLGGWGGLALRVLLPLAMLLAGGAALYTWQQNQRAAELVEIDSQLLTDDLP
                     IDAYLDRNFQSWLKKREAAE"
     gene            23014..23496
                     /locus_tag="VIG70_19135"
     CDS             23014..23496
                     /locus_tag="VIG70_19135"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEY8252709.1"
                     /translation="MTRFFGFLLTLILTTGALAEPPVDVARKPSAKPAAKAKPAAKAK
                     AQVRPAWAELTVEQQKTLAPLKADWESLEPERRRKWIGIAKRYPTMKVQEQERVQRRM
                     QAWAKLSPEQRRQARETYKQIVKAPPEKRANLRQQWAEYQALPEREREALTAEPRRRK
                     "
     gene            23501..23893
                     /locus_tag="VIG70_19140"
     CDS             23501..23893
                     /locus_tag="VIG70_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY8252710.1"
                     /translation="MSTPGLPRRLASMGYEAILLFAVAFFAAWIFFFASGGRDATAGA
                     LRYALQLFIAVVFASYFLWCWLRGGQTLAMKAWHIRLANVTPVSALLRLMLAALLVPT
                     GVSVIWALFDPERQFLHDRLAGTRLTNA"
     gene            complement(23898..25382)
                     /locus_tag="VIG70_19145"
     CDS             complement(23898..25382)
                     /locus_tag="VIG70_19145"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8252711.1"
                     /translation="MLDIVKCKPLIASILGCALAAPVALAHPPHSPYDNTVFAPIKKF
                     GPRVAVEVIASGLVSPLKGVTAPGERDKLYVVDQTGQGWKVDLDTKERTLFIDVSGRL
                     VKLGVLGENSFDERGFLGLAFHPDYRRNGLIYTYTSEPNAGAPTFPTTMTTRAADHQN
                     VVAEWRVQGGVVDMSSRRELMRVDHPQFNHDGGDLAFGPDDKLYISMGDGGGADDRDG
                     QLFVKATGSMPPVNEPIDGHGLDGNGQKLTNPLGKILRIDVDGRNSKNGRYGIPKDNP
                     FVGRGGGVLGEIWAYGFRNPYRFSFDSKHGTLFVGDVGQNDIEEVDIVRKGGNYGWPI
                     KEGTTFFNHNGDADGFAQREPLGPVPHGLIDPVAQYDTHHEGHSVVAGFVYRGRALHG
                     LYGRFIFGEFSTIFNFPTGPHDYGRLLHMSGDSNQHGLRSITEFHIVGGNSLGFAVLG
                     MGEDSDGEVYLTGNRSGLPFGTAGMVLKIVPAPDEGKDHHDRDD"
     gene            complement(25657..26736)
                     /gene="lptG"
                     /locus_tag="VIG70_19150"
     CDS             complement(25657..26736)
                     /gene="lptG"
                     /locus_tag="VIG70_19150"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY8252712.1"
                     /translation="MRIRTLERYLAKQIYGATAFVLLGFLGLFAFFDLIRELADLGKV
                     DYQLRQVFTFVLLSMPTHAYELFPVVVLIGTLYVLAHLASNSEYTVMRASGLSPRRAA
                     LVLAKIGLAFVVLTFVIGEWVAPWSEEQAQKVKLRAMSSMIGQDLMSGLWFKDEGSFI
                     NVREARQTNQLSGIRIYDFDADYRLQKITLAERADYAGKGIWQLRGVAQTQFTAQGPR
                     TQRQPEMEWRSRVSPDMLDALIVRPERMSAWALHKYTEHLAGNRQRTERYEIALWKKV
                     FYPIAALVMMALALPFAYMQARAGMVGVKVFLGIMLGIFFHMLNSLFSHIGLLQNWPP
                     LSAAVVPSAAFLAAAILMMWWVERR"
     gene            complement(26738..27814)
                     /gene="lptF"
                     /locus_tag="VIG70_19155"
     CDS             complement(26738..27814)
                     /gene="lptF"
                     /locus_tag="VIG70_19155"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEY8252713.1"
                     /translation="MIFHRALLREFAGLAGAVFMTLFAIAVTTRLIRLLGQAAGGKFP
                     SDAVFAFLGFFALGALPVLLSLTMFISVLLTMTRSWRDSEMVIWFGAGQPLTAWLKPV
                     MLFALPQIAVIAALSLFISPWAAQMAGQYASRLEQRDDVSRVTPGVFGETANRTRVFF
                     VESVSGDSSAVQNIFVSSIQQNRSGVSMSRSGHTETAENGDRFIVLEQGRRYEGAPGD
                     PEYRVTEFDRYAARIELKEGKEVTPTHKNLPTWSLIINPSNQNLGELLWRVGIPISAF
                     VLVLLAIPMSFVNPRAGRSANLLFALLTYIVYSNLLSVSQARVAQGRMDFGVGWWLVH
                     AGMVALLVVMFAQRMQLIRLRLGK"
     gene            27831..29330
                     /locus_tag="VIG70_19160"
     CDS             27831..29330
                     /locus_tag="VIG70_19160"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY8252714.1"
                     /translation="MEFSIKTLSPEAAKVGCVAVGVYQRNATGSELTAAARRLDQASK
                     GAVKRALRDLSGKTGSTLLLHALPGVAAERVLLVGLGDKSTTDAAYRDAVRAAANALK
                     ELDAKDAALFLVDMKIGSRPVSWNVRHAVLGVRDAFYRFDQLKTQKKPPAPALERVVL
                     PLSSKPELTQALTEAVATADGAALARTLGNLPANICTPAYLADEAKKLAREFKLQVEV
                     LERKDMEKLGMGALLSVARASHQPPKLIVLHYKGGANSKKTRAPLLALVGKGITFDTG
                     GISLKPAGEMDEMKFDMSGAASVLGAVRALAAMRAPVNVVGVIPACENMPGGAASKPG
                     DVVTTLSGQTVEILNTDAEGRLILCDALSYAERFKPDAVIDIATLTGAIVIALGHVAT
                     GLFANDQKLADEIRAAADEAWDRVWQMPLWEDYQEQLRSNFADMANIGGRPGGSITAA
                     SFLARFTTKLRWAHLDIAGTAWKSGRDKGSTGRPVPLLVRFALKHAGKK"
     gene            29327..29767
                     /locus_tag="VIG70_19165"
     CDS             29327..29767
                     /locus_tag="VIG70_19165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEY8252715.1"
                     /translation="MTSIDFYFNADDRLQVACRLAGKAIKQGQRLLIYAPDSETASRI
                     DKMLWTWPAIGFVPHCAVHAALAAETPVLIGSGEEAPDNCEVLLNLGADCPPHFERFT
                     RLLEVVGTDEEGKEKGRGRFRFYRERGYKITNHDLAQEMRQGHE"
     gene            29760..30092
                     /locus_tag="VIG70_19170"
     CDS             29760..30092
                     /locus_tag="VIG70_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252716.1"
                     /translation="MSDDSLDPLVKRVDALIGRQEGPRAKGEVPVLTEIVSEEKNPRR
                     AMDIASLEALARELERAVLERMAPELERIVGEALAGARAELKDSVTQMVREAVAASVA
                     RALAMPKE"
     gene            30093..32852
                     /locus_tag="VIG70_19175"
     CDS             30093..32852
                     /locus_tag="VIG70_19175"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY8252717.1"
                     /translation="MLEKSFNPGAIEEGWYRRWEDAGYFRHKDHATPAYCIQLPPPNV
                     TGTLHMGHAFQQTLMDALIRYHRMRGDNTNWVVGTDHAGIATEIVVTRQIEAEGKTKR
                     ELGREEFNRRVWQWKQQSGSTITRQMRRLGVSANWEYADTEGQKAGYFTADPKMSRAV
                     TEVFVRWYEEGLIYRGQRLVNWDPVLGTGVSDLEVDTDEEDGRIWEIRYPGEGFDIRV
                     ATVRPETMLGDVAVAVHPSDARYADLVGKKVRLPLTDRTIPVIADEYVDPKFGTGAVK
                     ITPGHDFNDYEVGKRHRLELINILTLDAHLNDKVPPKYRGLERFAARKQVVADLKAQG
                     FLVSEKPYKLRVPRSGRTGVIIEPMLSDQWFVKMESLAKDALGVVARGEVKFIPEHWT
                     ATYNHFLENIKDWNISRQLWWGHQIPAWYDEEGKLYVARSEAELQQKHPRKKLRRDPD
                     VFDTWFSSQLVPFSSLGWPDKTPDQEVFLPSSVLITGNDIIFLWVSRMIMSSRYFTGR
                     VPFRDVYINAIVRDAEGEKMSKSKGNTIDPLDLIDGISLQDLLKKSTIGLMKDEHKER
                     IEKYVRSHYSNGIPAFGADAVRFTFASLASFSITLNFDLGRCEGYRNFCNKLWNATRF
                     VLMNTDGKDCGTDDKLPVRLSFADRWIVSQLQRVEAEVEHGFADYRFDNVAAAIYRFV
                     WDEYCDWYVELAKLQLQDPDAAAQRGTRRTLVRVLEAALRLAHPLIPFITEELWQKLA
                     PLAGKKGETVMLQRYPKSQPEKIDEAAERDVAVLKEWTVAARNLRTEAKIPPGERVML
                     YRTADPAVSDPPEVAKAISSLARLSGFEKRDPLPDAPSPVAVVGGARIMLYKEIDPAA
                     ERVRLMKEIARLEGEIGKARAKLANPSFVERAPAKVVEQERTRVAGFEADLAKLKEQL
                     RKLGP"
     gene            complement(32821..33312)
                     /locus_tag="VIG70_19180"
     CDS             complement(32821..33312)
                     /locus_tag="VIG70_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252718.1"
                     /translation="MKARWIRLGAIGQQEFDAAYASIAAAQPRAAAPVLLWAEEAARY
                     PFALIAPLKFAPGRTSRWLSWGLAAAVATYRQFGVPAYLNDELFLHGRDIAQSRVLAL
                     GECAVIASSFLMRFPGSCVATPSGELEHAFRLRLEAQHGWEFDYSWPTEPEIRGRASV
                     AAL"
     gene            complement(33326..33814)
                     /locus_tag="VIG70_19185"
     CDS             complement(33326..33814)
                     /locus_tag="VIG70_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY8252719.1"
                     /translation="MNRALLLGGLLAAAAVNAADATFTVKVLTPETALKAAQAALKSC
                     RERGFQATVAVVDRMGVVQVLLRDRFAGPHTPDMATAKAYTAVSFRTNTTALAEATQP
                     GRPSSGIRNRPGVAAVGGGMMIEAGGSLLGGIGVSGAPGAREDDACAAAGIAAIREDI
                     EL"
     gene            complement(33811..34038)
                     /locus_tag="VIG70_19190"
     CDS             complement(33811..34038)
                     /locus_tag="VIG70_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY8252720.1"
                     /translation="MNFDKELDARGLNCPLPILRTKKALTDMHSGQVLKVLATDPGAV
                     KDFQTFSRQTGHELVSHAEANKEFTFFMRKK"
     gene            34056..35084
                     /locus_tag="VIG70_19195"
     CDS             34056..35084
                     /locus_tag="VIG70_19195"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEY8252721.1"
                     /translation="MKEADRPADWAFDPHATRPVTSEPDGADGGKLPGWLLMIAQLPV
                     DDPSSRMRVLRTLESLGAAVARDGVFLLPDTPSNRQSLETLTEYIAKVAGTANVLQTS
                     ALSPAQHQGFVRLFDRSARYENLIKTVESLRVGFGHSDPSAISRVLHKQRREFEAIAA
                     LDFFPNAARERAEQALAAADNDVRKLLFPAQVPVALAPGQGLLGRTWVTRKPLWADRL
                     ACSWLIRRFIDPEATLTWLEKTQSLSADAIAFGFDGAHFANSETRVTYEEMLVKLDLA
                     KNPALVKIGSIVHFLEVRGTPVAEAAGVQTLLQGAQRRSNSERELLQEAEKTFDLLYE
                     AYYQAPKN"
     gene            complement(35203..36636)
                     /locus_tag="VIG70_19200"
     CDS             complement(35203..36636)
                     /locus_tag="VIG70_19200"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY8252722.1"
                     /translation="MRFGVAALAFFACSVFAQALPDLGGGADGGLSPQMERRIGESIM
                     RDIRFREPNYLDDPEIADYLGSLGGRLAQNAAGARQDFEFFAIREHTINAFALPGGFI
                     GVHTGLLMASESESEAASVLAHEIAHVTQRHIARMLSQQQQMQLPMLAALAAAILLGR
                     SRPDLASGAAAAASAGAVQTQLSYSRDYEREADRIGFQALEASGFDVHAMGTFFEKMQ
                     RGSRAMDDGSMPGYMRTHPITTERIADAQNRAASLPYKQHLDSAEFQLVRAKLRAESG
                     DARDTVEYFQGSVREKRYASESAARYGLASALLRARRPKEAQAELQRARAAGAAGPMI
                     ETLAARVRLGLGDRAGAAALLGEAHARFPNSRPLLYAQADALQEAGRNQQALTLLTDS
                     LRLYPRDARLHQLQAKNYAALGKRSLQHQAQAEVYVLQGSLPAAIEQLQLSRAAGDGD
                     FYQNSVVDARLKELRAQHARDMKDAKR"
     gene            36674..37180
                     /gene="moaC"
                     /locus_tag="VIG70_19205"
     CDS             36674..37180
                     /gene="moaC"
                     /locus_tag="VIG70_19205"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY8252723.1"
                     /translation="MTPQRLTPQSLTHFDAKGSAHMVDVGAKAATHRVAIASGRIVMK
                     PATLRLIAAGAAKKGDVLGVARIAAVQAAKRAAELIPLAHPIALTRVAVDFTLDKRTA
                     SVRIVSRVECRGQTGVEMEALTAAAVGLLTIYDMVKAVDRGMTLTEIRLEEKRGGQSG
                     VWPRRKLK"
     gene            37186..37623
                     /gene="soxY"
                     /locus_tag="VIG70_19210"
     CDS             37186..37623
                     /gene="soxY"
                     /locus_tag="VIG70_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEY8252724.1"
                     /translation="MNNIRRTVLAAVGLLLLKPLAAAAAWNQEAFGAKSAADALKGLG
                     SANPAPSKDIVIEAPQIAENGAVVPIEISSNIPGTTSIAVLIEKNPFPLTGKFEFKEG
                     AAPFVKLNVKMGETSDVRVVAMAGGKSFAATREIKVTIGGCGG"
     gene            37625..37933
                     /gene="soxZ"
                     /locus_tag="VIG70_19215"
     CDS             37625..37933
                     /gene="soxZ"
                     /locus_tag="VIG70_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEY8252725.1"
                     /translation="MAGPMKMRATLAGGNTAAVHTEIRVLMTHPMTTAQMKEGLHHIQ
                     NVVVKQNGRVVVEAEISQAVSRNPVFSFRLKGGAKGDKIEVSWLDNKGESNKIETAVA
                     "
     gene            37930..38721
                     /gene="soxA"
                     /locus_tag="VIG70_19220"
     CDS             37930..38721
                     /gene="soxA"
                     /locus_tag="VIG70_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HEY8252726.1"
                     /translation="MRSCFLLCGVLACTPLFAQDTKKEIQKYQQMIADGSPVELFELE
                     GEALWKKAQGPKNASLEKCDLGQGAGVLKGAYARLPRYFKDADRVMDLETRLLHCMTT
                     LQGRSREEATKRVFGNENQPSEMEYLSAYVAAQSRGAKLAAGTSHPREKDAYELGKAL
                     FFHRAGGWDMSCASCHGEEGKRIRMQDLPVLSKREFARPIMATWPAYRVSNSQFKTMQ
                     WRINDCYRQMRYPEPSFASDATIALNTFLTVTAAGEPYRGPGTKR"
     gene            38718..39311
                     /gene="soxX"
                     /locus_tag="VIG70_19225"
     CDS             38718..39311
                     /gene="soxX"
                     /locus_tag="VIG70_19225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEY8252727.1"
                     /translation="MTRVLLSSFLIFPLLAGAQTTAPDVQRVLQRDFKARGQATMDRV
                     VQDGVQRVCTESDDRPPAALAKQMEADQLKTIAFPEGSLIGDWKRGEGIAQNGRGLQW
                     TDPAGAGGGGSCYNCHQLSPQETSHGTLGPSLLRFGATRGNGAEMQRYVYGKIYNAKA
                     YNLCSHMPRLGYSGTLTPEQIKDLVGLLLDPASPVNK"
     gene            39405..41030
                     /gene="soxB"
                     /locus_tag="VIG70_19230"
     CDS             39405..41030
                     /gene="soxB"
                     /locus_tag="VIG70_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HEY8252728.1"
                     /translation="MQRREFLQLLAVAAAAGASLRPRTGEAQAAAELYDVPPFGNVSL
                     LHITDTHAQLLPVYFREPAGKEFLEKYKTRSGTQFAYAFTRVDFERLARRYGKVGGFA
                     HLATLVKRLRATRPHALLLDGGDSWQGSATALWTGGADMRGAQQRLGVDFMTAHWEFT
                     YGQKPIQDSPVKVLAQNVRTADFEDPVFEPYALRSLNGIPVAIIGQAFPYTPIANPRH
                     FVPDWTFGIQEGRMQKIVDEARAKGAKAVVLLSHNGMHVDLKLAGRVRGLDAILGGHT
                     HDAVPRPVRVGRTLVTNAGCNGKFLAVLDLQVGASGVTDSRYRLLPVFSRLLEEDKDM
                     AAYVSSVREPYREKLGEKLALTEGLLYRRGSFNGSFDELILQASLKEKDAEIAFSPGF
                     RWGTTLLPGDAITLEHVMDQTAITYPQCTSNELSGEQIKHILEDIADNVFNADPYLRQ
                     GGDMVRAGGVRYALEPGARIGARITDLRIRGKPLEATKRYKLTGWAPVAEGITGEPIW
                     DVVTRHLRAAKTVPPLEVNRPRLIGVKDNPGLT"
     gene            41027..42571
                     /locus_tag="VIG70_19235"
     CDS             41027..42571
                     /locus_tag="VIG70_19235"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY8252729.1"
                     /translation="MKRAAVLALVVLAIAAGGWYWYQSRDGDAELRYRVAKVERGSLA
                     AVVVASGTLNAVTTVQVGSQISGQVKEIFADFNTAVKKDQVIAQIDPSTFELRVNQAR
                     ADLDSAQGAVAVARAGMAAQQAEVARVKVTLAEARRDVERKKALVDKKFISAAELDKA
                     VVVVDTTREQLNAVQAQIKVGEAQVSSARAAVKQRESLLKQAQVDLERTIIRAPVDGT
                     VILRNVDAGQTVAASLQAPVLFTIAQDLRDMQVEAAIDEADVGRLRVGMPAGFAVDAF
                     PRRNFAGEIKQIRKSPVNVQNVVSYTVVISAANPDLALLPGMTANVRIAVENRANALK
                     VPNAALRFRPAGTVDVKAPADAPPQQQGGQAVQEFRNRIQQELKPSDAQRAQLEEIFT
                     ESRQKFARVRDMKGDGNRRKEAERIRAETNGRIAEILTPEQRPAWERLLAEAGSRGPS
                     TSGRVYVLENAEPKPLDVRLGLSDGMSTEVLSGLAEGTEVIIGTADARGSAPAAGGGL
                     PRARFF"
     gene            42571..43263
                     /locus_tag="VIG70_19240"
     CDS             42571..43263
                     /locus_tag="VIG70_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8252730.1"
                     /translation="MLIHTRDLVKVYRMGGGEVRALDGVSVDIAEGEFVAVMGPSGSG
                     KSTFMNVLGCLDRPTSGEYVLGDKRVSELAGDELAIVRNRRIGFVFQQFNLLARTPAS
                     ENVELPLVYAGLPKKQRLEKAKAMLARVGLADRGHHHPAQLSGGQQQRVAIARALVTE
                     PLLILADEPTGALDSRTSLEIMALFQELNRQGMTVVLVTHEPDVARFARRIIAFRDGK
                     VVQDERNEALAA"
     gene            43260..44492
                     /locus_tag="VIG70_19245"
     CDS             43260..44492
                     /locus_tag="VIG70_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8252731.1"
                     /translation="MSFVALLRIALRALAVNKLRSALTMLGIVIGVGAVIVMIAVGGG
                     AQARVEEQIRALGSNLLLVMPGSTTAGGVRMGFGSGSTLTEDDVGAINREIPEALGAP
                     ALRGSAQVIWGNANWSTQIFGVTPEYLDVRQWPLASGRSFEPGEMAGAAKVCLIGATV
                     ARQLFGAADPLDQSIRIKRVPFTVIGVLDVKGQSLMGTDQDDLVLVPIKTARSRVLGT
                     ASAARNRTVGTIWVKVADGLDTKVVEEQVRSLLRQRHRIQAGADDDFSLRNLAEVMAA
                     QEASSRVLALLLAAVASVSLLVGGIGIMNIMLVSVTERTREIGLRMAVGARTRDILGQ
                     FLVEAVTLSLIGGLIGVALGIGGSVAIAQLAGWRIVLSPEAVGLAVAFAFAIGVFFGF
                     YPARKAARLNPVEALRFE"
     gene            complement(44494..45270)
                     /gene="tfpZ"
                     /locus_tag="VIG70_19250"
     CDS             complement(44494..45270)
                     /gene="tfpZ"
                     /locus_tag="VIG70_19250"
                     /inference="COORDINATES: protein motif:HMM:NF041437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfpX/TfpZ family type IV pilin accessory
                     protein"
                     /protein_id="HEY8252732.1"
                     /translation="MKKSETALKRPFTRWRAAGAHLLISACVAAAVLGVMLALWYPPP
                     LFEAMGGSGLALILVGVDVVIGPALTLVVFRSGKPGLKFDLAAIAVFQLAALVYGCHI
                     ISLARPAFIVFVKDQFQIATVVELPPERLATARYPQYRSPSWSGPVLVFGDWPKDFAE
                     QQQLLFASLGGEDLQHFPQYYAPYEQGRDQILARAQPLSEVRRVEPVVAKVIDAWLAR
                     SGVEEQRLRYVRLRGRNAWVAVLIDRLTAEPVKMLVVEKV"
     gene            45345..46256
                     /locus_tag="VIG70_19255"
     CDS             45345..46256
                     /locus_tag="VIG70_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8252733.1"
                     /translation="MNCLVLGGGGFIGSHLALALADAGHRVRVFDRPQGRFLPLLEGC
                     EVFTGDFLEPRDLERALSGTEVVCHLIATTVPKSSNEDQVSDVAANVVGTLRLLELCR
                     SRGVRRIVFPSSGGTVYGIPRTTPIGETHPTDPISSYGIHKLTIEKYLHLNQVLNGLD
                     YRVLRIANAYGERQRTETAQGAVSVFLERSLRGDALQIWGDGSVVRDYIYVGDVVQAF
                     LRILAYEGEHRIFNVSSGVGVSLNQLVDEIARAIGRRPAVEYAPARRFDVPVNVLDAS
                     LARKHLGWSAATPLAEGLRRTYDWMRR"
     gene            46253..>48342
                     /locus_tag="VIG70_19260"
     CDS             46253..>48342
                     /locus_tag="VIG70_19260"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252734.1"
                     /translation="MIRAERNLFLISFLILFFELAAIRWFAATVVFLTFFTNIVLLAC
                     FLGMSVGLLAARRPQNLVQAALPIAMLAFAAAVATHLAYLNWAEQLTVSVSDQASPRM
                     IYFGTEYRPADPSRWIVPMWAVGGAFFTLLALTFVGLGQVMGRAFDAIPDRVRAYTID
                     VLGSLAGIAAFAAMSYLELPPTVWFVPILLLMLYFAGWRKPVQLTAAAVVYAMVGLSA
                     HVLTAQGTQIFWSPYYKVSYDPPFQVISTNDIGHQNMVAVGQSGPAYLLPHLLNRDAG
                     GAPFGEVMIIGAGSGNDVASALRSGARHVDAVEIDPRINRLGRELHPERPYADPRVSV
                     HLDDGRSFTRRTERKYDLAVYAVVDSLVLHSGYSSVRLENFLFTREAFEDVKRVLKPD
                     GVFVMYNFYRQGWLAARLKRMAQEAFGVEPVVVSLPHRDHIPDDDSVLHGFTFLLVSA
                     DPGRLAALRARFEKGESFWIHPYPPMNEAVNGFRAEAPGPKWHRIGPTQVDPSVDGRV
                     PSDDWPQAYLRRPEIPWAPIGQGMAAIAVISAVILFAFAPARRMRPNWQMFFLGAGFM
                     LLETKGVVHMALLFGSTWVVNSVVFFAILVMILAANLYVVKIRPNKLTVYYALLVLAL
                     LVNALVPMNVFLSLSPLWRTLASCVVVFAPVFFAGVIFATAFRESRQPDVDFGWNVAG
                     IVLGGLSEQLSLVLG"
BASE COUNT         8130 a        16264 c        16082 g         7866 t
ORIGIN      
        1 gatagcgctt ggcgtcgacg tgcagccgga tcggaccgag aaccgcctcg gggaagtggg
       61 gccgcaggaa cggcagcgcc acgtactggt gcaggtcgtc gatgccgcgc agcgtaggcg
      121 tctcgccgcg gtcgttcagc aggtggccga tgtcgtgcag cagcgcggcg cacaccagcg
      181 cgggcgcggc gccggcctgc tcggcgaggt gcgcgctctg cagcgcgtgc tcgagctgcg
      241 tgatgccctc gccctcgtac tcgcgcccgc ctttcgcctg atacaggccg atgatctgct
      301 cgattaccag ggcagcgagt acgtcttcac gttggtaaag ctcttgcagg cttcctgcac
      361 cccttccttg tagcccaggc ccgaatcctt gatcccgccg aagggcgtga gctcgaggcg
      421 gtagcccggc acctcccaca cgttcacgct gccgacgttg agctccttca cgaagcgcat
      481 gatgtcgtcc agccggttgg tgcagacgcc cgaggacagc ccgaaatcgg tcgcgttcac
      541 caggcggatc gcctcgtcca cgctcttgaa cgtcatcacc ggcgacaccg ggccgaaggt
      601 ctcgtattta accaccggca tctcgggctg cacgcggtcg agcaccgtcg gcgagtagag
      661 cgcgccctgg cgcgcgttgc cgcagagcag ccgcgcgccc ttggaaaccg cttcgttcac
      721 cagcccctcg aagctggacg ccgcctgctc gctgatcacc gtgcccatgt cgaccttggg
      781 gtcgagcggg tcgccgtagg accacgcctt ggttttttcc acgagacgag aaacaaattc
      841 ttcagcaatt ttttcgtgga ccagaatcct cttcaccgcg gtgcagcgct gcccggagtt
      901 cttgtacgag ccgttcgcgg caaggtcggc cgcctttgcc ggatccgcgt cgtccatgat
      961 cacgagcggg tcgttgccgc ccagctcgag cacctggcgc ttgtacaccg ccttggccgc
     1021 gatgtacttg ccgatgggga cgccgccggt gaaggtgacc atgtccacgt ccgggttggt
     1081 cagcatctcg tcggcgatct ccttcgggtc gccggtcacc acctgcagca tctcgggcgg
     1141 cagcccggcc tcgtagagca cgtcggcgag caccagcgcc gcgagcggag tcttgttggt
     1201 cggccgcagc accatgcggt tgttggtcgc gaccgagggc gcgactttgt ggatcacctg
     1261 gttgagcgga tgattgaagg gcgtgatcgc cgagatcacg cccatgagcg gctcgcgcat
     1321 ggtgtagacg cggcggctct tgccgtggtg cgtgaggtcg cacgagaacg tctggccgtc
     1381 gtcgaccagc gcctggttgg cggcgaacag cagcacgtcg gaagcgcgcc cgacctcgta
     1441 ggtcgaatcc ttgatgcaca accccgactc gagcgtgatg ccgcgcgcga tctcgtcccg
     1501 ccggctcgcg atgatctcgc ctgcgcgcat caggatcttg tagcgctcgt agcgcgtgag
     1561 cttcggcttg tagtcgcgcg cgacgcggaa cgcgcgcttc acgtcgtcga ccgtcgcctt
     1621 ggcaatggtg gcgacgactt cgccggtgta gggatagcgc acctcgatcg tgcgatcgcg
     1681 gtacaccttc tcgccggcga tgcgcagcgc ctcgcggcgg ggggattcga cagtccgggt
     1741 tctggcgtgc atgacttcct tcactcctaa ggaactatag cagcgcgcag cacgcgccgg
     1801 tcggcggcgt cctgcatcgc cttgccgact tcgtcgaggc gatacttgcc gtcgaccagg
     1861 tcgtggaacg gaaagcgctt gcggttggcg accacgaaat cgagcgcttc ggggaggttt
     1921 cgggggtggt agttgtgcac cccgcgcagc gtgacgagct tgcgcaggat ctggttggcg
     1981 tcgatgcaca cgaacgcatc cgggttgacg acgccgccga gggcgtagcg tccgccgatg
     2041 cgcaacgcct gtatccctgc cggaatgacc tcggggttgc cgcaaacctc gatcaccacg
     2101 tctacgcctt cgacgccttt caggtcggcc accgcctggt cgacgccgaa gcgcgccgac
     2161 atctcgcggc gcgccgcgac cgtgtccacg ccgatcacgc ggcgagcgcc acgggccttg
     2221 cagaccgccg ctgcgtagag accgaggagt ccaaggccct ggatgacgac agtatcgccc
     2281 ttggccacct cggccttctc gacgatgcag accaccgtgg ccacgccgca gttgatcggc
     2341 gttgcctctt cgtcggtgag ctcgggcggg agcttcagaa tccaggagcg cggcaggatg
     2401 tagcagtact cgccgaagcc gccgtggtgg tgcgggtcgg tcgtcaccgc catgtggccg
     2461 tacttgtcca cccccggcgc cttttgcggc agctccagcg tttcgctgcc cggcggcacg
     2521 aaatattcgc tccaggtaat gcggtcgccc accgcaagcg aatcccccct catatcgcgc
     2581 ttgatgtccg aacccatttc aacgaggttg ccgatgatct cgtggccgag caccccgggg
     2641 cagggattgg gacggtgccc gaggtaggaa tggatgtcgg agcggcagat ggtcgacatg
     2701 cgcaccttga ccagcgcttc gtcggcacgc ggcggccgca gcgggtacga gcgcagctcg
     2761 aatggcttgc ccggggcgtc gtacaccgcc agcttgccgc ttgatgccat gcgcgcttca
     2821 ccagccattg agggcgtatt cgaagatctg gtagctcttc agcgtgctga cgccggcctt
     2881 taatttgtag tcgtcgttca gcggcacgct caggataaac ggcaccttcg cttccgagac
     2941 gccgccgtgc gtgcgcaagc ggtgcccctc gaggccggac aggtcgtgcg ccgcggacga
     3001 gccgccgatg cagacgttgg cggtcgagat caccaccacg tcggcttcgc gatcagccgg
     3061 aagctcgaac acgcggcagg cagtctcctt gtccatcacc gattcgatgc cctcgaggcc
     3121 gtggatggcg tcgatcacct gcttgggcct ggcctttccg gtgcaccaga cgcgcacgaa
     3181 gccgccgagg gcgccgtggt gggcgacgaa cgcgtcggtg atcgggcaga tcacgcgcgt
     3241 atcgcccttg ccgaattttg cgtcgagcat gtcctgcagc cagatgacgt ttggcttgcc
     3301 tttggcgtcg gacttgtcgt tcatgccgtg gtcggcggtc agcgcgacga ccgcgccgag
     3361 cgcctccagc cggccgaaac agcggtcgag tgcctggtag aagcgcttcg cctccgactc
     3421 ctcgggcgcg tacttgtgct ggacgtagtc ggtgagcgag aggtacagca ggtcgggccg
     3481 gtcgcgctcg agcagcttga tgcccgcctc gagcacgaac agcgacagct ccatcgagta
     3541 catgccgggc tgcggcatgc ccaccagcgc gagcacgttc tcgatcccgt tctctttcaa
     3601 ggtggccttg gaagcaaatt ccgaagagaa gctgatgttg cccttcgaca cgtcgaggtt
     3661 cttgccgagc tgcttcctca gcttgtcctt ggcggtgatg gaaaccacct tcgcgccggc
     3721 tcttgcgaac tgcgagagga tggtgtcgcc gcgtaggagc tccggcccgg tcatcaccac
     3781 cgcctgcccg gtgctgacat cgaggtagaa attgcccgag atgccgtggc gcgacgccgg
     3841 cgtgccggtg atgatcgaca tgttgttcgg gcaggtgaag ctcggcaccg tgccgtcggc
     3901 cacggtgctg aagccctgcc ggatgaagcg cgcgatgttg gggatcgagc cgtccctgag
     3961 gaactgctgc aggtaggccg gatccccgcc gtcgatgcac accacggcca ccggcttttt
     4021 cggccagcgg tagcgggtgc cgtttacagt gacagtgtct ttcatgagcc ttcggcggcg
     4081 cccgcgatca gctgcgtgag cttgttggtt tccttggtgt aggggaccac ggtgctggca
     4141 aatttgcgct gcgactccag cacgcgcttg aaaaacgcgt ccttggcctc gtattgcgcc
     4201 agcaccttct tcgccgactt gatgaacgcg gaagcgtagc cgctcggcgc gtcgaacatc
     4261 ttgacgccct tgtcccgggt gagcacctgc agcgccttcg agttctcatc gacgaaatac
     4321 aggatagcct cgaagatcga cgtcgtcatc gccgtctgca cgatcgcctg caggtcgggc
     4381 gtgagctcgc gccacttcct gccgctgatg acgacatctg cgatgtcgat cgcctggtgc
     4441 agaccctgca aggagtagtt cttgaacacg tccgacagtc cgagcttcag gtcgttggcc
     4501 ggattgatcc actcgacgcc gtcgatcagg ccgcgctcgg ccgccgggat cacctccgca
     4561 cccgccaggt tcatcggcac cccgcccatg tccttcagta catcggaggg caggcccgac
     4621 gacatgcgga agcgcatctt ggtgtactcg gcgacggtct tgggcacgcg cttcacccag
     4681 ccgaaggcct ccgggccatc cggggcgtag aggaacgcct gcacgtcggc cttgaggatc
     4741 ttctggtagt actcgaggta gagctcgcgg ccgccgccgc gcaggtacca cgacaggtgg
     4801 ttcatctggt cgaggccgct gccggcgcca ccgatgggcg cgctgaacaa tccgccggcc
     4861 gggtgcttgc ccgtcgcata gtgcgtccac cattggccgg cctcgacaat ccccttgttg
     4921 acgccgtcga ggatctcgaa cggcgccacg atcgcgccgc tcggcagcac ctcgatcttc
     4981 agccgcccgc ccgacatgct gtcgatattc gcgaccagcc ggctcagcag ctccatgtgc
     5041 ggggtacccg cggggttggc gctctgcagc ttccaccgga tctgcggcgc ctgcgctctt
     5101 gcaatcatcg ggaatccgag catcgcggcg ccggtggttg cggcggtggc ggcgccgagg
     5161 aacttgcggc gtgatgccgc cgccctggcc tgcgtattca gtttcgcgct tctcttgctc
     5221 atgctgccct cctcgatcag gaacggttta gctacggatc agccgaacag caggttcggc
     5281 agccacatca cgatctgcgg aaaaatatag agcagcgcca ccgcgatggc ctgcaggaac
     5341 atgaacggca tcatcccctt gtagatttcc agcagttccc actccggcat gattcccttc
     5401 agatagtagg ccgacagcgc gaccggcggg gaaagccagc aggtctgcag ggtcacggcc
     5461 agcaggatgg agaaccagat catgtcggtc ttaagctgcg tgaggaccgg caggaagatc
     5521 ggcacgatca ccaccacgat cggcacccat tccagcggcc agccgaggat gaagcagacc
     5581 accagcagcc cgagcaggaa gaccgcgggc ggaaagtccc acgccagcat ggtttcggcg
     5641 atgaacttgg gcgttcccat cgcggagaaa accacgccca tgaaggtcga cgccgcgagc
     5701 aggatcatca ccatgctcgt cgtctcggcg gtgcggaagg cggattcctt gatgatcttg
     5761 aaggtcagcc gcccatggga tgcggtcacg cccagcgccc cgaggcagcc gagcgccgcg
     5821 ccttcggtcg gcgtggcgag cccggagatg atcgagccga gtgttaccag gatcaaggtc
     5881 accggcggaa tcaggccgac gaagaactcc cgcaccagga agctcgtcgg cactttcagc
     5941 tcttcgtcgg agagcgcggg ccccaatgag gggttcagca tcacgcgtgt catggtgtag
     6001 gaaaggtaga taaacgcgag cagcaggccc ggaatgatcg cggccgcata gagccgtgaa
     6061 acggagaccg agaacaccgg ccccatgacc accagcatga cgctcggcgg gatcaggatg
     6121 cccagcgtgc cgccggcgca gatcgtgcct gcggacaggc cggtgttgta gcccgccttc
     6181 ttcatcaccg gtgcggccat caagccgatg atcgtcaccg acgcgcccac gatcccggtc
     6241 gccgccgcga acagggtcgc cgtgaaaatg gtggccatgt agagcgagcc cctgatgcgg
     6301 cccgagatga gctgcaccgc gcggaacaag cgctccatca agccggcgct ctccagcacg
     6361 tagcccatga acgtgaacag ggccaccgcc gcgagcacgg tatcggtcat ggtgccgaaa
     6421 tacgagagcg tcatcaggtg aaacacccgc tcgccgagcg cgatgtagcc gaacaccatg
     6481 ccgagcgtga tcagcgtgaa ggaaatcggg aaccccaggc agatgacgcc gaacagcagc
     6541 accagcagga tcaggccgta gatttcgagg ctcaaggccg tgcccggccc tgcgcgtcag
     6601 gtgatctcac cgggcgcctc gcggtgacgg atatcgacgc cggtgagcag cagcttcacc
     6661 gacttgagca gctcagagat gccctgcagc agcagcagcg ccgcgctcag cgggatgacg
     6721 gacttgaacc agtacgccgg cggccgccag gtcgtcatca ggcgctcccc caggtcccag
     6781 gactcggccg cgaaatccca gctcagccac acgaacatca ccatgcccgg aagaaaaagc
     6841 agcaggtaac agatcacgtc gacccacgcc tgggtgcgcg gcgcccagtt gccgtagaag
     6901 aaatcggtcc ggatgtgctt acccaggtac agggtcatgg cggccgccat caggtagtgc
     6961 gtgccgtaga gcatggtcgc gacgtcgagc gcccagatcg acggggcgta ggcgtagcgc
     7021 acgataacct cgccgcccag catcgcgatc atcgggaaga tcagccacgc gatcgctttc
     7081 cccgaccaga tactgacctg gtcgatgacc gcgatcacgc ggtccatcgc cctcagcgcc
     7141 gtccgggtcg ccactaggcc gccgcgcgct tgaagtcgtg cagccgcgag cggctgttga
     7201 tcacgtgctc gtagagcagg cgctcggcaa gccgcgcgtc gcccctcgcc accgcgtcca
     7261 cgatcgccgc gtgctccttg gtcgagaccg ggatgatgtc gacgttgcgc tggatcgctt
     7321 cgcgccggta gagattgagc tcgttgacca cgcggcggta gttcgctgcg agcgcccggt
     7381 tcccggcggc ctcggcgagc gcctcgtgaa actcgatgtt gagcggaaag taggcgtcgg
     7441 gatcgcgctt gcggccgacc gcctgcatgc gcttgacgat cgcgcgcagg cgctcgagct
     7501 gcggcgccgt ggcgcgcttc gcggcaagct tgccgatctg cgcctcgagc gcggcgcgca
     7561 cctcgtagat ctcgttcgcc tcctcgagcg aaacctggcg cacgaacacg cctcggttct
     7621 tctcggtgtg cacgaggccc gcctgctcga gcgcgcggaa cgcctcgcgc accggcccgc
     7681 gggagatccc catggcggcg gcgagctcgg cttcgttcag cttggcgccc gggacgagct
     7741 ccccggcgac gatgcggcgc tcgatttccc tctggacgag ggaggtcagc gactcctggc
     7801 gcagcagctc gatcggggag agggccaccc gcgattgtct ggccacggat tgccgattgt
     7861 caacaattta caatcttgcg gcgctcgggg tcgtagggga cgcgctcgct caagcgcgcg
     7921 gcgtggcgca cgccgaagct ttcgatctcg aagcctgtgg ccttggcaag ctccatgggc
     7981 acgaaaccgt acgcgatcga cttgccgatg gtgtgaccga aaccaccgct cgtcacggtc
     8041 tcgatcaccc ttccttcgtg caggatcgct tcgccgccgt gcaacggcac gaagccgtcc
     8101 atgagaaagc aagcgagttt tcgcctcgtt ccctctttgc gaatcttctg cagcgccgca
     8161 cggccgatga agtcgccctt gtcgagcgcc acggcgaagc cgagtccggt ctcgtacgga
     8221 ttcgcatccg gcgagagctc gctgctccag tagcggtagc ccttctccag ccgcagcgaa
     8281 tcgaccgcgc ggtatccggc attggcgacg ccgatgtcct ggccggcatc ccacagggct
     8341 tcatagacat gcagcgcgta ctcgctcggc acgtgcaact cccagccgag ctcgccagcg
     8401 taggtgagac gcaacgcgag caccggcgcg aagccgacgc ggatctcgcg cgcggccagg
     8461 taggggaagg cggcgttcga gaggtcgcct tcggcgaccc gcgacaggag ctcgcgtgac
     8521 tgcggcccct ggacgttgat caccgcacgc gaggaagtga cttccttgaa ctgcacctcc
     8581 gcgggcagct ggcgcgagat ccactgcccg tccctgacgg cgaaaccgga tcccgtaacg
     8641 atgtagaaat gctccgccga gagcctcacc accgtgagat ccgcctcgat gccccccttc
     8701 tcgttgcaga gctgcgtgta gaccacggag ccggcgggct tctcgaggtc attggcgacg
     8761 attcgctgca gcgccgcgaa tgcgccgcgg cccgtgatct cgaacttgct gaaagagctg
     8821 aaatcgaaca gcgcgacgcg ctcgcgtacc gcgcggtgct cggccgcgac gtgctcgaac
     8881 cagttcggct tgccctcaaa actgggtttg tcctgcattc caggagaaga aaaccagttg
     8941 ggccgctccc agccgaactt cgacccgtag accgcgccgc gctccttgag cgtttgccag
     9001 agcgggctgc ggcgcgcatc gcgcgcgacc gtcaactcct cgccgggata gtgcaggtgg
     9061 tagtacgagc cgtaggcgtg caccgcgcgc tcatgcagcg cgcgcgtgcc cgaatggaag
     9121 tcgccgaagc gccgcaggtc gaagggccag aggtccattt ccgggtagcc ctgcacgatc
     9181 cactcggcca tggcgcgccc ggctccgccc gcagcggcga tgccggaagt gaagccgcac
     9241 gcgacgaaca cgttgtcgcg ctccacagcc tgtcccatga tcggctcgcc gtcgggcgat
     9301 accgggatcg ggccgttgat gagctgcttc agcccgagct cgccgaacgc cgggatgcgc
     9361 tccgcggtgg cgagcgcgat ttcctcgaag cggtccatgt tcggcggcag cagctcctgc
     9421 gcgaactcga acggcacgcc cgcggcgtgg aacgacggcg tgcccatctc ccatccgccg
     9481 accgcgaagc cgccgacctc gggcttgaga tagaagttcc ggtccgggtc gcgcagcgtc
     9541 ggaagcttcg gatttcgttc cttcatcggc tcggtgacca tgtattggtg ctcgagcgcc
     9601 gccgcgggaa tgcgcacgtc catcagcgca ccgactgcgc gcgaccagat gccggcggcg
     9661 atgaccacga tctcgcagtc gattgcgcct ttgtcggtca ggacctgcgt gattcgtcga
     9721 tccttggatt cgaacccggt gacgcgtgtg tcctgcagga tcttcacgcc agcggaacgc
     9781 gcgcccttcg cgagcgcttg cgcgagactc gaaggatcga cgtagccgtc gctcggcacc
     9841 cacgccgcgc ccgcgacgcc gtccagggtg atgtagggga acttcgcctg cgcctctttc
     9901 ggggagatca gctccatctc gaagccgaag ccgcgcgcgg tggtcgcggc gcgtttcatc
     9961 tccgaccagc gctcggccga cgaagcgagg cgcacgctgc cgacttcgtg ccagtcgatc
    10021 gcctggccgg tttcgcgctc gagcgtccga taaagggcga cgctgttctg catgaggcgc
    10081 gtgaggttgc gcttgctgcg cagctggccg accaggccgg cggcgtgcca ggtcgagccg
    10141 tgggtcagct gcgccttctc cagcagcagg atgcttttct tcgaaaagcg ggaaaggtgg
    10201 taggcgatcg agcagccgat cgccccaccg ccgatgatta cgacgtcagc gtcttgacgt
    10261 accactgcac gaacgcgtcg acgttgtatt ccttgtgctt cgagtaaggc ccgggctcgt
    10321 aggcggtcga gtcgacgccc ttctgctggc gctcgcagat cgcccagtcc tgctcggagg
    10381 tgagctgcca gaacggcatc agcttgccga gctggtaatc gcggccctcg accgcgtctt
    10441 cgtgcacgag ccacgtgacg cgcgcgagcg tcttgcgcgg cccggcggga agcaggagcg
    10501 tgctcacgcc gtggtcgcag ctcgagtgat tccagaaatt gggcagcgtg cgcgcgcgca
    10561 ccgtgccgac gtctgggtcg cggtattcgc ccatcagcgt ggagacctgg gcgccgtcca
    10621 tcgactcgct cacccagcct tcgaccagcg cggtgcggtt ggccgagtac cagatgccgc
    10681 gctcggcatc cggaaactgc gtcatgccgg tgtcgcgatg cgacacggcg aggcccgcag
    10741 ccgcccattt ggtttcactc ttcttgacca cgcgatcgat cgccgcgcga acttcggtcg
    10801 tcgtatcgtc ggagttgtaa tggtcccagt tcgccttgat gtactgcggg tggttgacgt
    10861 tgcagtggaa gcactcgcgg ttgttctccc acacgagctt ccagttcgcc gcgatttcgt
    10921 agtcgacctg cttcgccacc ttggcccgct cgaacccctg cggccgcagg tagggcgcca
    10981 tcagctcgcg cgcctgagcg aaagggggcg gggcttgcgc caatgaaatg aagatcaggc
    11041 cttcgacggt ttcacaagaa acttgcttta gtgaaaaacc cttgcgatcg aagtcatgca
    11101 tgccgcggca gaaaaccagc gcgccggtcg tgtcgtaggt ccactggtgg tacgggcaca
    11161 ccaggcgccg gctcgagccg cgccccgggg cgcagatctg cgagccgcgg tggcggcaga
    11221 cgttgtgcag cgcccgcacc ttcccgtcct cgccgcggat cacgatgatc gagtcggtgt
    11281 cgacctgcag cgtcagccag tcgcccgggc tgcggatctc gcagccgtgg ccggcgaata
    11341 gccacccctt gcgccaaatt ctctcgatat cagcgcgata caccgcttcg tcgcgatgga
    11401 agggacgcgg caggctccag cccgggcggt acgccgccag gtgctgcgcg agctcccctt
    11461 gcaacccctg cttcatccgg taacaatgcc tttcgacaat aagaataatc aactgcgtat
    11521 cagcagcctc actgggagac tatccatgtt gaagtatctg ttgcaactcg ccgcgctgac
    11581 cgccctcgcc ctgagcgcat cgctcgccct ggcgcagcgc accccgatcc tggtgtacac
    11641 ggcgatcgag accgaccagc tcaaggccta caaggacgcc ttcgaggccg cggtcaagga
    11701 cgtcgagatc cgctgggtgc gcgactcgac cggcgtcgtc accgccaagc tgctcgccga
    11761 aaaggccaat ccgcaggccg acgtggtgct cggacttgcc gcaaccagcc tgctggtgct
    11821 cgagcaggag ggcatgctga cgccttacgc gccgcaaggc gtcgagaaac tcaaccagcg
    11881 cttcgtcgac gcgaagaagc cgccgatgtg ggtcggcatg gacgtgtggg gcgcgaccat
    11941 ctgcttcaac acggtcgagg cgaagaagcg caacctgccc aagccggaaa gctggaagga
    12001 cctgctgaag ccggtctaca agggccagat caccatgccg catcccgcat cgagcggcac
    12061 cgggtttctc gacgtgaccg cgtggctgca gctcttcggc gaggctgagg gctggaaata
    12121 catggacgcg ctgcacgaga acatcgcgca gtacgtgcac tcgggctcca agccctgccg
    12181 ccaggcgggc gccggcgagt tcgtcatggg catctcgttc gagtaccgcg gcaacgccac
    12241 caaggcctcg ggcgcgccgg tggacctgat cttcccgaaa gaaggcgtgg gctgggacat
    12301 ggaagcctcg gcgatcatga aggcgagcaa gaagcaggcg gccgcgcgca agctgctcga
    12361 ctggtcggtg acgcgcggcg cgaacgagct gtacgcgaag aactacgcga tcgtcgccct
    12421 gccaggcgtg gccaagccgc tgcccaacct gccgtcgaac tacgaatcga tgctggtgaa
    12481 gaacgacttt gcgtgggcgg cgaagaaccg cgaccgcatc ctcgccgagt gggaaaagcg
    12541 ctataacgca aaagccgaac cgaagtgacg ctaccgcgtt agccgtcgaa ggagtttcca
    12601 agcacttcgg cggcttcgcg gcgctcagcg acgtcagcct ggcggtcggc cagggagaat
    12661 tcgtctgcct cctcggcccg tcgggctgcg gcaagaccac gctgctacgc atcatcgccg
    12721 gcctcgagac gcagagcgtc ggccggatcg tgcagaacgg ccgcgacatc tccgtgctgc
    12781 cgccggcgca gcgcgactac ggcatcgtgt tccagtccta tgcgctgttt cccaacctca
    12841 cggtggctga caacgtcggc tatggcctgg tcaaccggcg catggcgcgc gccgaggtgc
    12901 gcctccgggt agcggagctg ctcaagctgg tcgggctgcc gggcagcgag ccgaagtacc
    12961 cgagccagct ttcgggcggg cagcagcagc gcgtggcgct cgcgcgtgcg cttgctacgt
    13021 ctccgggctt acttttattg gatgaaccct tgtcggctct ggatgcgatc gagcgcgtgc
    13081 ggctgcgcgg cgaggtccgc ggcctgcaga agaaggtcgg cgtcaccacg atcatggtga
    13141 cgcacgacca ggaagaggcg ctgtcgatgg ccgaccgcgt ggtggtgatg aacaagggcg
    13201 cgctcgagca ggtcggcacg ccgcgcgaga tctacgagca gccggcgacg ccgttcgtcg
    13261 ccgacttcgt cggccgggtg aacgtgctga gcgcggtgtc gctcggcgcc ggccgctacc
    13321 aggccggccg cctcgcactc tcggcggtca acggcgccac gccgcatgcc gcgggcacgc
    13381 gggtgcggct ctacctgcgt cccgaagacc gggtcgtgga aggcaacctg gacagcgaat
    13441 cgaaccagat gcacgggcag atcgcgaaag tcgagttcct cggcgggcac tgcctggccg
    13501 aaatccaggt tcccgagctc gagggccagg cgctgagcgt gttcttctcg ctcaaccagg
    13561 tgcgcgagct cggcatccgc gagggcgccg ggctcaggct cgcgctgcgt cccgaccgca
    13621 tccgcgtatt cgcgtcatga gcgctgcgtg agcgcggtcc tggccgccga gcggccccca
    13681 ctgctcggcg tcgagttcat cgccagggcg ttgctcgttc tcaccggcct cgggctcgcg
    13741 ctgttcctgc tcgcgccgat ggccgcgatc ttcgtacgcg cggtccagga cagcaacggc
    13801 aactacgtcg gcctggtgca cttcgcgaac tacttcgcca cgccgtcgct ggcgcgcgcg
    13861 gtatggaatt cggtctgggt gtcgctggcg gtcgccgcca tcaccgtgcc cgcggcgttc
    13921 atgttcgcct atgcgctgac gcgcagctgc atgccgttca agggcacgct gcggctgatc
    13981 gcgctgattc ccctgctcgc cccgtcgctg ctgccggcaa tctcgctcat ccagtggttc
    14041 ggcaaccagg gggcgctgaa gggaccgatg caggcgctcg gcatcagctc gatctatggt
    14101 ccgccgggga tcatcctggc ggaagtgttc aactgctttc cgcacgcgct gatgatcctg
    14161 atcgcggcgc tggcgctggc cgacgggcgc ctgtacgaag ccgccgatgc gctcggcacg
    14221 cgccggctgc gccgcttctt caccatcacg ctgccgggct gcaagtacgg cctgctgtcg
    14281 gcgttcaccg tggtgttcac ctacaccatc agcgatttcg gcgcgcccaa ggtgatcggc
    14341 ggcaacttca acgtgctcgc cgtggacgtg ttcaagcagg tgatcggcca gttcaacttc
    14401 tcgatcggcg cggtggtcgg cctgctgctg ctcgtgccct cggtggtggc gttcggcgtc
    14461 gactggatgg tgcgccgccg gctcaccgcc aatctcacgg cgcgctcggt gccgttcatc
    14521 ccgaagccgc gcccgctgtt cgacaacctg atgctcggat acagcgcgct ggtgtgcctg
    14581 ctgctgctcg cggtgctcgg catggcggtg tacacctcgc tcatccacct gtggccgtac
    14641 aacctcggct ttaccctcaa gcactaccac tacgcgctgg tcggcggcga ctacgcgccg
    14701 gcgttctgga acagcctgaa gatgggcgcg tggaccgcgg tgatcgggac ggtgttcgtg
    14761 ttctgcaccg cgtacctgct ggagaagacg cgcggcctcg ggccgctgcg gccggtgctg
    14821 cggctgctcg cgctgctgtc gatggcggtg cccgggctgg tgctgggcct cggctacatc
    14881 atgtttttca accatccgca gaatccgatc aacttcctct atcagacgat ggcgatcctc
    14941 gcgatctcca ccgtcgtgca ctactacacc tcgggccacc tcaccgcggt caccgcgctg
    15001 aaggcgctcg acgacgagta cgagtcggtc tcggcctcgc tcaaggtgcc gttctacaag
    15061 acgttctggc gcgtcacggt gccggtgtgc ctgcccgcta tcctcgagat cgcgcgctac
    15121 tatttcgtgg tgtcgatctc gaccctgtcg tgcgtggtgt ttctgtacgc cccgcacacc
    15181 atcctcgccg cggtcgcgat catgaacatg gacgaggccg gcgacgtcgg ccccgcctgc
    15241 gccctcggca ccctggtggt gctcgcatcg gtcggcgtgt gcctgctcta cgcgctcgcc
    15301 acccgcgtgc tgctcgccaa gtcccaggcc tggcgcttca acctggagaa agcatgaaag
    15361 agcccatcct ccttacccct ggtccgctca ccacttcgac cgccaccaag ctcgccatgg
    15421 tgcgcgactg gggctcgcgc gacaccggct tcatcgaaat caaccgccgc gttcgcgcca
    15481 tgcttttgga cgtcgtgggt ttgaaagaat cgcacgtctg cgtcccgctc cagggcagcg
    15541 gcactttcgc ggtggaagcg atgctcggca ccctggtgcc gaaagacggc cacgtcctgg
    15601 tcccgctgaa cggcgcgtac tgcaagcgca tccagcgcat ctgcgggatt ctcggccgca
    15661 aggccaccgt cctggagttc gaagagcgcg aacccgtcaa gccagcggcg gtggacgcgg
    15721 ccctcgcgcg cgacccgtcg atcacgcacg tggcgctggt gcactgcgag accagcaccg
    15781 gcgtgctgaa cccgctggcg gaaatcgccg cggtcgtggc gcgtcacaag aagagcctgc
    15841 tggtcgacgc gatgagctcg tttgcggcgg tcccgatcga aggcgcgtgc gacgcgctgg
    15901 tcgcggcctc cggcaaatgc ctcgaagggc cgccgggaat ggggtttgcc ctgattcgca
    15961 agactgtcct cgagaaatgc gcgggcaatg cccactctct ggtactcgac ctgcacgacc
    16021 agtgggtcaa catggagaag accgcgcagt ggcgcttcac gccgcccacc gtgatcgtgg
    16081 ccgcactgca tgcggcgctc gagcagttcg ccgccgaggg cggccgcgcg gcgcgcggcg
    16141 cgcgctaccg ccgcaattgc gaggtgctca ttgagggaat gacgcgcctc gggttcaagc
    16201 tgttcctgga ccccaagcac caagcgccgg tgatcgtgac cttccacgcg ccgagcgacc
    16261 cgaactacga cttccagaag ttctacgacc gcgtgcgcga gaagggcttc gtgctctacc
    16321 cgggcaagct cacccaggtc gacacgctgc gcgtcggctg catcggcgcg atcgacgagc
    16381 acgtgatccg cgccgcggtg cacgcgatcg ccgacaccgt cgccgagcag aacatccggc
    16441 tgcatgaaac tggcgctgtt cgatctcgac aacacgcttc ttagcggcga caccgacgtc
    16501 gagtggctcg atttcctggt cgagcgcgga gtgcttcccg cttccgagcg cgcggcaaac
    16561 cacgagatgg accgccggta ccgcagcggc gaggcggcgg ccctcgagta cgtgcgcttc
    16621 tacctgcgct tctatcccgc gcacgacatg tccaccctcc tcggcttgcg ccagcgattc
    16681 gtgcaggcgc gcatcgagcc gcgcatgctg ccggcggcgc gcgagctgat ccggagccac
    16741 ggcaaggatc taatcgccat catcaccgcc accaaccgct tcctcaccga gccgatcgcc
    16801 gccgccttcg gcgtcgagca cctgatcgcc accgagccgc agatcgagca cggccgcttc
    16861 accggcgacg tcgtcggcgc cccctgcatg cgcgagggga agatcgagca tctcgagcgc
    16921 tggctcgctg cgcgcgacaa ggtgctcggt aatttcgaag agagctggtt ctacagcgac
    16981 tcgatcaacg acctgccgct gctcgagcgc gtgacgcatc cggtcgcggt cgatccggat
    17041 atctcgctcg aaaaaatcgc gcgcgaccgc ggttggcgca ggctgactct gagaggatga
    17101 ccgtcatgaa acccaaaact cctgccaaga cgcgcagtac gccgaatcgc aagcaggtcc
    17161 gcgaggccac tgctccacgg ctcaacgtac cgcgtcaatc caagcgccca acggaaagaa
    17221 gcaaacagaa ccgcagccgc ggctgaagcc ggcagcctgg cggtcactcc tccggcagca
    17281 ccggccgcgc gcggtggccc atcgcccaca tgacgtagcc ggagacggcg tagcccatga
    17341 cgagggcgaa caggacgagc gacgggctgg aggcgatgat ggagagcgcc accacgcagg
    17401 cgaggatcac ccagagcggc acgcgcttcc tccagttcac ttccttgaag ctccagtagg
    17461 gcacgttcga caccatggtg acgccggcga acaccgcgac cccccaggtg agcggcgcaa
    17521 gccaggcgtg ctcgcgcggg acgaagccga aatcgaccag cgcccagacg aagcccacca
    17581 tcaccgccgc gcccgacggg ctcggcagac cctggaagta gcgcttgtcg accaccgcga
    17641 tgttggcgtt gaagcgcgcc aggcggatgc cggtgccggc gcagtagatg aaggcgccga
    17701 tccagccggc ggtgcccagg tccttcagca cccagatgta ggccatgagc gccggcgcgg
    17761 cgccgaacgc gaccatgtcg gcgatcgagt cgtactgcgc gccgaactcg ctctgcgtgt
    17821 tggtccagcg cgcgacgcgc ccgtccatgc cgtcgagcac catggcgatg aagatcgcga
    17881 tgcagccgat gtcgaagcgg ccgttcatcg cctgcacgat ggcgaagaag ccgcagaaca
    17941 gcacgcccgt ggtgaagagg ttgggcagca ggtagatgcc tcggcggcgc aggcccacgc
    18001 gcgctcccgc gcgcgggtcg gcgcgcgggt ctacgcgcgg gtcgacgtcg gggtctacta
    18061 cttcctccgg ctcgtattcg ggggcgtcag ccatgcgagc acgctctccg cggcatacac
    18121 cttgtcgccg atcgccgcct tcacctcggc gtcgagcggc aggtagacgt cgacgcgcga
    18181 tccgaagcgg atgaagccga agcgctggcc gcgctcgagc tcggcgccgg cgccaacata
    18241 gcacaggatg cggcgcgcga tcagccccgc gacctgcacg caggtcacgt cgtggccgtc
    18301 gcgtgtgcgc agccagagcg cgttgcgctc gttctcgagc gacgccttgt cgagcgccgc
    18361 gttgacgaac gcgccgggaa agtaccagcg ctccttcacc gtgccgtcga ccggcgagcg
    18421 gttcgagtga acgttgaaca cgttcatgaa cacgctcact ttcagcgcgt cgcgcttcac
    18481 gtacggatcc tgcgcgcggc tcacctcgac cacgcgcccg tcggcggccg acagcaccgc
    18541 gcgcggatcg tcgggaatgc tgcgcggcgg atcgcggaag aactgcagga tgaagatccc
    18601 ggcgagccag aacgggatcg accaccacca cccgaggaac accccaatca aaagggaaat
    18661 tccaagcgcg atcagcacga aaacccaccc ttcacgggcg atgaacgggt gggggtagcg
    18721 ccgcgacact acgctagttc ttggacgtgt ccaccaggcg attgcgcttg atccagggca
    18781 tcatctcgcg cagccgcgcg ccgaccttct cgatcggcag ctcggcggtc atgcgacggc
    18841 gcgagagcag cgtcggcgcg ccggcgcggt tctcgagcac gaactccttg gcgtactcgc
    18901 ccgactggat gcgcccgagc acctccttca tgcgcttgcg cgtgccctcg tcgatgatct
    18961 tgggcccgga cgtgtactcg ccgtactcgg cgttgttgga gatcgaatag ttcatggtgc
    19021 cgatgccgcc ctcgtacatc aggtcgacga tcagcttcag ctcgtgcagg cattcgaaat
    19081 acgccatttc cggcgcgtag cccgcgtcga ccagcgtgtc gaagcccgcc ttcaccagct
    19141 cgacgcagcc gccgcacagc acggtctgct cgccgaagag gtcggtctcg gtctcttcct
    19201 tgaagttggt ctcgatgatg cccgccttgc cgccgccgat cgccgcggcg taggacagcg
    19261 ccacgtcgcg cgccttcttg gacggatcgc ggtgaaccgc gatcagcatc gggactccgc
    19321 cgcctgccgc gaaggtcgag cgcaccgtgt ggcccggcgc cttgggcgcg accatccaca
    19381 cgtcgaggtc ggcgcgcggc tggatcaggc cgtagtggat gttgaagccg tgcgcgaagg
    19441 cgagcgaggc gcctttcttg atgttcggtt caatgtactc ctggtagacc gcggcgtggt
    19501 gctcgtcggg cagcagcacc atgacgatgt cggcgccctt gatcgccggg gcgagctcgg
    19561 cgaccttgac gccgcctttc ttcgccttgt cccaggaggc gccgcccttc ctcagggcga
    19621 cggtcacctt cacgcccgat tcctgcaggt tctgcgcgtg cgcatggccc tgcgagccgt
    19681 agccgataat ggtgaccttc ttgcctttca ccaatgaaag atcggcgtcc ttgtcgtaat
    19741 aaaccttcat ttgcttttcc cctgaagaac tagactcgaa ggacgcgatt gccgcgtccg
    19801 atcccgcttg cccctgtacg caccgtctcg atgatggcgc cctggtcgag cgacctgatg
    19861 aacgcgtcga gcttggcgcc gtcgccggtc agctcgatgg tgtaggtgtt gtccgacacg
    19921 tcgatgatcc ggccgcggaa gatgtcggcc atgcgcttca tgtcctcgcg ctccttcagg
    19981 ccggcgcgca ccttgatcag catcagctcg cgctcgatgt gctcggcttc cgacaggtcg
    20041 atcaccttca ccacctcgat cagcttgttc agctgcttgg tgatctgctc gaccacgtcc
    20101 tcggagccgg tggtgacgat ggtcatgcgc gacatcgagg catcctcggt cggcgccacc
    20161 gtcagggtct cgatgttgta gccgcgcgcg gagaacaggc ccgagatcct ggacagtgcg
    20221 cccgcctcgt tctccacgag gacggaaatg atgtgtctac tcacagatcc tcgtagagga
    20281 tcatctcggt gatccctttg ccgccgggga tcatcgggta gacgttctcc ttctggtcgg
    20341 tgaggaagtc gaggaacacg agatcgttct tcgcggcgaa ggcttcgcgc agcgcgcctt
    20401 cgacgtcgga gggcttctcg atcagcacgc cgcggtggcc gtagccctcg gcgagcttca
    20461 cgaagtcggg cagcgcgtcc atgtaggact cggaatagcg gttgccgtgg aagaactgct
    20521 gccactgccg caccatcccc atgtacttgt tgttcaaatt gacgatcttg atcggcaggc
    20581 ggtactgctt gcaggtcgac agctcctgca ggcacatctg gatcgaggac tcgccggtga
    20641 cgcaggccac cgtggcgccg ggattggcga gctgcacgcc catcgccgcc ggcaggccga
    20701 agcccatggt gcccaggccg ccggagttga tccagcgccg cggctcgttg aacttgtaga
    20761 actgcgccgc ccacatctgg tgctggccga cgtcggacgt gacgaaggct tcgcccttgg
    20821 tcacctcgta gagcttctcc agcacgaact gcggcttgat gatcgccgac ttgcggtcgt
    20881 agcgcaggca gtccttcgcg cgccattcgt tgatttgttt ccaccagggg gcgatatcgc
    20941 gcggcgcggt gcctttcaga agcttcagca gatcctcgag cacgtcggga atgttgccga
    21001 cgatcggcac gtcgaccttc acgcgcttgg agatcgacga cgggtcgatg tcgatgtgga
    21061 tgatcttgcg cgggttctgc gcgaagtggg ccgggttgcc gatgacgcgg tcgtcgaagc
    21121 gcgcgccgat cgcgagcagc acgtcgcagt gctgcatcgc catgttcgct tcgtaagtgc
    21181 cgtgcatgcc gagcatgccg aggaactgct tgtcggtcgc cgggtaggcg cccagtccca
    21241 tcagcgtgtt cgtgcacggg aagcccaggt ttctgaccaa ttccctcaac aaaagagagg
    21301 cgtccgagag caccacgccg ccgcccgtgt acaccatcgg gcgctcggcc tcgagcagga
    21361 gctgcaccgc tttcttgatc tgcccggcgt ggcccttgcg caccgggttg tacgagcgca
    21421 tctgcacgct ctcggggtac tggaactcgc aggcgtgctg cgtcacgtcc ttcgggatgt
    21481 cgaccagcac cggccccggg cgcccggtgg ctgcgatgtg gaatgccttc ttgatcgtgg
    21541 aggcgaggtc cttcacgttc ttcaccagga agttgtgctt cacgcacggg cgcgtgatgc
    21601 cgaccgtgtc gcattcctgg aaagcgtcga ggccgatcgc gtgcgtcggc acctgtccgg
    21661 tcagcacgac gagcgggatc gagtccatgt acgccgtggc gatgccggtg acggcgttgg
    21721 tcacgcccgg gccggaggtg acaaggcaca cgccgacctt tttcgaggag cgcgagtagc
    21781 cgtcggcggc gtgcaccgcg ccctgctcgt ggcgcaccag gacgtgcttc accttgtcct
    21841 gcttgaacag ctcgtcgtag atgaacagca ccgcgccgcc gggatagccg aagacgtact
    21901 cgaccccctc ctcctggagg cagcgcacga caatctcggc gccggtaact gtagttgttt
    21961 tagcgcggtc cataagtaaa gcattgaaga ataaaagctt gatgcagtgc ggtcaagctg
    22021 cgccgcgcct tggcgcgggt gccttttgat accatcggcg gccaaacaac agaggggcca
    22081 ccctggcatc ccgcagcgag ctttcggcct tcctggaggg cgtcgagaag cgcgcgttca
    22141 agcaggcggt gttcgccgtg cgcgacgaaa aagcggcgct cgacatcgtg caggacgcga
    22201 tgctgcggct ggcggagaag tacgggcacc tgccggcagg cgagctgccc gcgcttttcc
    22261 accgcatcgt gcagaacgcg atccgcgact ggtaccggcg gcagaaggtg cgctcgacat
    22321 ggaccacgca gctctcttcg ttcggcgagg acgacgaaca ggatcccctc gaaaccctgc
    22381 agcccgccga agggtcagag gtacaagagg gtccggccgg acaactggag caggctcaag
    22441 tgcttgaaat aattagacaa gagattaacc gcttgccaga acgtcaacgg gaagctttca
    22501 tcctgcgtta ttgggaggaa ctggatgtgg ccgcaaccgc gcgggcgatg agttgctcgg
    22561 agggcagcgt taaaacgcat tgctcccggg ctgcgcacgc tttgcaggta gcgctcaggg
    22621 cgcgcggcat cacgctcgac ataaagacat gaacgaacag caattgcgtc agcttctgaa
    22681 tcagggaatc accgtcaggc cggagattgc ggcgcgcctc gagcaagcgc gcaagcaggc
    22741 tcttgcgctg cagcggcccg tacccgcgcc tgcgctcgct tgggccggca acgcggtggg
    22801 gcacctcggc ggctggggcg gcctggcgct gcgcgtgctg ctcccgctcg ccatgctgct
    22861 cgccggcggc gccgcgctct acacctggca gcagaaccag cgcgccgccg aactggtaga
    22921 gatcgactcg cagcttctca ccgacgacct gccgatcgac gcctatctcg atcgcaactt
    22981 ccagagctgg ctgaaaaagc gcgaggccgc ggaatgacgc gctttttcgg gttcctgctg
    23041 accctgatct tgaccacggg cgcgttggca gagcctccgg tcgatgtcgc caggaagccc
    23101 tccgccaagc cggcggccaa ggcgaagccg gcggcgaaag ccaaggccca ggtgcgccct
    23161 gcgtgggcgg agctcaccgt cgagcagcag aagaccctgg cgcctctcaa ggcggattgg
    23221 gaaagcctcg agcccgagcg ccggcgcaag tggatcggca tcgccaagcg ctaccccacg
    23281 atgaaggtgc aggagcagga gcgcgtgcag cggcgcatgc aggcatgggc caagctctcg
    23341 cccgagcagc gtcggcaggc gcgcgagacc tacaagcaga tcgtcaaggc cccgccggag
    23401 aagcgcgcca acctgcgcca gcagtgggcc gagtaccagg cgctcccgga gcgcgagcgc
    23461 gaagccctca ccgcggagcc gcgaagaagg aagtgaccgc gtgagcacgc ccggcctccc
    23521 gcggcggctc gcatccatgg ggtacgaggc gatcctgctc ttcgccgtcg ccttcttcgc
    23581 cgcgtggatc ttcttcttcg cgagcggcgg ccgcgatgcc accgccggcg ctctgcgcta
    23641 cgcgctccag ctcttcatcg cggtcgtctt cgcttcctac ttcctgtggt gctggctgcg
    23701 cggcggccag accctcgcca tgaaggcctg gcatatccgc ttggcgaatg tcacgcccgt
    23761 gagcgcccta ctgcggctga tgcttgcggc tctcctggtg cccacgggcg tttccgtcat
    23821 ttgggcgttg ttcgatcccg agcgccagtt tctgcacgac cggctcgccg gcacacggct
    23881 gaccaacgcc tgagcgccta gtcgtcccga tcgtggtgat ccttgccctc atcgggggcg
    23941 ggaacgatct tcagcaccat gccggcggtg ccgaacggca ggcccgagcg gttgccggtg
    24001 aggtacacct cgccgtccga gtcctcgccc atgccgagca ccgcgaagcc gagcgagttg
    24061 ccgccgacga tgtggaactc ggtgatcgag cgcaggccgt gctggttcga atcgccgctc
    24121 atgtgcagca ggcggccgta gtcgtgcggc ccggtgggga aattgaagat agtcgagaac
    24181 tcgccgaaga tgaaccggcc atacaggcca tgcagcgcgc ggccgcggta gacgaagccg
    24241 gcgacgaccg aatgcccctc gtggtgcgtg tcgtactgcg cgaccgggtc gatcagtccg
    24301 tgcggcaccg ggccaagcgg ctcgcgctgc gcgaagccgt cggcgtcacc gttgtggttg
    24361 aagaaggtcg tgccttcctt gatcggccag ccgtagttgc cgcccttgcg gacgatgtcg
    24421 acctcctcga tgtcgttctg gccgacgtcg ccgacgaaca gcgtcccgtg cttcgagtcg
    24481 aacgagaagc ggtacggatt gcggaagccg taggcccaga tctcgcccag cacgccgccg
    24541 ccgcgcccga cgaaggggtt gtccttgggt atgccgtagc ggccgttctt cgagttgcgg
    24601 ccgtcgacgt cgatgcgcag gatcttgccg agcggattgg taagcttctg gccgttgccg
    24661 tccaggccat gaccgtcgat cggctcgttc accggcggca tgcttccggt cgccttgacg
    24721 aagagctgcc cgtcgcggtc gtcggcgccg ccgccgtcgc ccatcgagat gtagagcttg
    24781 tcgtcgggcc cgaaggcgag gtcgccgccg tcgtggttga actgcgggtg gtcgacgcgc
    24841 atcaactcgc gccggctcga catgtcgacc acgccaccct gcacgcgcca ctccgccacc
    24901 acgttctggt ggtcggcggc gcgcgtcgtc atcgtggtcg gaaaggttgg cgcgcctgcg
    24961 ttcggctccg aggtatacgt atagatcagg ccgttacgcc ggtagtccgg atgaaacgcg
    25021 aggcccagga acccgcgctc gtcgaacgag ttctcgccca gcacgcccag cttcaccagg
    25081 cggccggaga cgtcgatgaa gagcgtccgc tccttggtat cgaggtcgac cttccagccc
    25141 tggcccgtct ggtcgacgac gtacagcttg tcgcgctcgc cgggcgcggt cacgcccttc
    25201 agcggcgaga ccagtccgct cgcgatcact tcgacggcga cgcgcggccc gaacttcttg
    25261 atcggtgcga acaccgtgtt gtcatagggg gaatgcggcg ggtgcgcgag cgccaccggc
    25321 gcagccagcg cgcacccgag gatggacgca atgaggggtt tgcatttcac gatgtcgagc
    25381 atgggttctc tccttggttt tggcttttcc cacgccgccg cggggacgcg gcggcaacta
    25441 caaaccctcg acggctgaat gcgttgtcgc aaaaagtgtg cgcccgaggg gcgcgcgaac
    25501 tcaagcaaat ttcaacaacc gcgagaactg cgcgccaaga tcctgacgaa ccgggcaaag
    25561 atcgccccta gggtgttctc ctgttgtccg ccaccgctct gcaccaagct gggaataaag
    25621 cggcatcaaa cgatgtgcag gtttacagtc ctcgcatcac cttcgctcca cccaccacat
    25681 catgaggatc gcggcggcca ggaacgcggc gctcggcacg accgccgccg agagcggcgg
    25741 ccagttctgc agcaggccga tgtgggaaaa gaggctgttc agcatgtgga agaagatgcc
    25801 gagcatgatg ccgaggaaca ccttgacgcc gaccatgccg gcgcgcgcct gcatgtaggc
    25861 gaagggcagc gcgagcgcca tcatcaccag cgcggcgatc gggtagaaca ccttcttcca
    25921 cagcgcgatc tcgtagcgct cggtgcgctg gcggttgccc gcgaggtgct cggtgtactt
    25981 gtgcagcgcc caggccgaca tgcgctccgg ccgcacgatc agggcgtcga gcatgtcggg
    26041 gctgacgcgc gagcgccatt ccatttcggg ctggcgctgg gtgcgcggcc cctgcgcggt
    26101 gaactgggtc tgcgccacgc cgcgcagctg ccagatgccc ttgccggcgt agtcggcgcg
    26161 ctctgccagc gtgatcttct gcaggcggta gtcggcgtcg aaatcgtaga tgcggatgcc
    26221 cgagagctgg ttggtctgcc gcgcctcgcg cacgttgatg aacgagccct cgtccttgaa
    26281 ccacagcccc gacatcaggt cctggccgat catcgagctc atcgcgcgca gcttcacctt
    26341 ctgcgcctgc tcctccgacc agggcgcgac ccattcgccg atgacgaagg tgaggacgac
    26401 gaacgcgagc ccgatcttcg ccagcacgag cgccgcgcgc cgcggcgaca gccccgaggc
    26461 gcgcatcacc gtgtactccg agttgctggc gaggtgcgcg agcacgtaca gggtcccgat
    26521 cagcaccacc acggggaaca gctcgtaggc gtgcgtcggc atcgacagca gcacgaaggt
    26581 gaacacctgg cgcagctggt agtcgacctt gccgagatcg gcaagctcgc ggatcagatc
    26641 gaaaaaggcg aacaggccga ggaacccgag cagcacgaac gcggtcgcgc catagatctg
    26701 cttcgcgaga tagcgctcca gcgtgcggat gcgcaagcta cttgcccagc ctgaggcgga
    26761 tcagctgcat gcgctgggcg aacatgacga ccagcagcgc caccatgccg gcgtgcacca
    26821 gccaccagcc gacgccgaag tccatgcggc cctgcgcgac gcgcgcctgg ctaacgctga
    26881 ggaggttcga atacacgatg taggtgagca gcgcgaacag caggttcgcc gagcgccccg
    26941 cgcgcgggtt gacgaagctc atcggaatcg cgagcagcac tagcacgaac gccgagatcg
    27001 ggatgcccac ccgccacagc agctcgccca ggttctggtt gctcgggttg atgatcagcg
    27061 accaggtcgg gaggttcttg tgcgtcggcg tgacctcctt gccctccttc agctcgatgc
    27121 gcgcagcata gcggtcgaac tcggtaacgc gatactccgg gtcgccgggc gcgccctcgt
    27181 agcgccggcc ctgctcgagc acgatgaagc ggtcgccgtt ttccgccgtc tcggtatggc
    27241 cgctgcggct catgctgacc ccgctgcggt tctgctggat cgagctcacg aaaatgttct
    27301 gcaccgcgct cgaatcgccc gacaccgatt cgacgaagaa cacccgcgtg cggttggcgg
    27361 tctcgccgaa cacgcccggg gtcacgcgcg acacgtcgtc gcgctgctcg agcctggagg
    27421 cgtactggcc ggccatctgt gccgcccacg gcgagataaa gagcgatagc gccgcgatca
    27481 ccgcgatctg cggcagcgcg aacagcatca ccggcttcaa ccacgcggta agcggctgac
    27541 cggcgccgaa ccagatcacc atctcggagt cgcgccagct gcgcgtcatg gtgagcagca
    27601 ccgagatgaa catggtgagc gacagcaata ccggcagcgc gccgagcgcg aagaaaccga
    27661 ggaaggcgaa caccgcatcc gaaggaaact tgccgccggc ggcctggccg agcaggcgga
    27721 tgaggcgcgt ggtcaccgcg atcgcgaaga gcgtcatgaa cacggcgccc gcgagaccgg
    27781 cgaattccct gagcagcgcc cggtgaaaga tcatgcgata ctttcggccc gtggaattta
    27841 gcataaagac gctctcgccc gaggcggcga aagtcggctg cgtggcggtt ggcgtgtacc
    27901 agcgaaatgc cacgggcagc gagctgaccg ccgccgcgcg gcgcctggac caggcatcga
    27961 aaggcgcagt caagcgcgcg ctgcgcgacc tctcgggcaa gaccggctcg acgctgctgc
    28021 tgcatgcgct tcccggcgtc gccgccgagc gcgtcctcct ggtgggcctc ggagacaagt
    28081 cgacgaccga tgcggcgtac cgggacgcgg tgcgcgccgc ggccaatgcg ctgaaggagc
    28141 tcgacgccaa ggacgcggcg ctgttcctcg tcgacatgaa gatcggctcc cggccggtga
    28201 gctggaacgt gcgccatgcg gtgctcggcg tgcgagacgc cttctaccgt ttcgaccagc
    28261 tcaagaccca gaagaagccg cccgcgcccg cgctcgagcg cgtggtattg cccttgagca
    28321 gcaagcccga attgacccag gccttgacgg aagcggtcgc gaccgccgac ggagccgcgc
    28381 tcgcgcgcac gctcggcaac ctgccggcga acatctgcac gccggcctat ctcgccgacg
    28441 aggcgaagaa gctcgcgcgc gagttcaagc tccaggtcga agtgctcgag cgcaaggaca
    28501 tggagaagct cggcatgggc gcgctgctgt cggtcgcccg cgcctcgcac cagccgccga
    28561 agctcatcgt gctgcactac aagggggggg cgaatagcaa gaagacgcga gccccgcttc
    28621 ttgccctggt gggcaagggc atcaccttcg ataccggcgg catctcgctc aagcccgccg
    28681 gcgaaatgga cgagatgaag ttcgacatgt ccggcgcggc ctcggtgctc ggcgcggtgc
    28741 gcgcgctcgc ggcgatgcgc gcgccggtga acgtcgtcgg cgtgatcccg gcgtgcgaga
    28801 acatgcccgg cggcgccgcc agcaagccgg gcgacgtggt caccaccctg tcgggccaga
    28861 cggtcgagat cctgaacacc gacgccgagg ggcgcctgat cctgtgcgat gcgctctctt
    28921 atgcggagcg cttcaagccc gacgcagtga tcgacatcgc caccctcacc ggcgccatcg
    28981 tgatcgcgct cggccacgtg gcgaccggcc tcttcgcgaa cgaccagaag ctcgccgacg
    29041 agatccgcgc cgcggccgac gaggcctggg accgcgtctg gcagatgccg ctgtgggagg
    29101 actaccagga gcagctgcgc tcgaacttcg ccgacatggc gaacatcggc ggccgccccg
    29161 gcggcagcat caccgccgca agcttcctcg cgcgcttcac gaccaagctg cgctgggcgc
    29221 acctcgacat cgccggcacg gcatggaaga gcggccgcga caaaggctcc accggccgcc
    29281 cggtgccgct cctcgttcgt tttgctttga aacacgccgg aaagaagtga caagcattga
    29341 tttttacttc aacgcggacg atcggctgca agtagcctgc cggcttgccg gcaaggcgat
    29401 caaacaaggc cagcggctgc tcatctacgc gcccgacagc gagaccgcga gccgcatcga
    29461 caagatgctc tggacctggc cggcgatcgg cttcgtgccg cattgcgcgg tgcacgcggc
    29521 gctcgccgcc gagacgccgg tgctgatcgg ctcgggcgag gaagcgccgg acaactgcga
    29581 agtcctgctc aacctcggcg ccgactgccc gccgcacttc gagcgcttca cgcggctcct
    29641 cgaagtcgtc ggcacggacg aggaagggaa ggagaaaggc cgcggccgct tccgcttcta
    29701 ccgcgagcgc ggctacaaga tcaccaacca cgacttagcg caggaaatgc gccaaggcca
    29761 tgagtgacga ctcattggat cccctggtca agcgcgtcga cgctttgatc ggccgccagg
    29821 aaggcccgcg cgccaagggc gaggtcccgg tcctgaccga gatcgtgagc gaggagaaga
    29881 acccgcggcg cgcgatggac atcgcctcgc tcgaggcgct cgcgcgcgag ctggagcgtg
    29941 cggtgctcga gcgcatggcg cccgagctcg agcgcatcgt cggcgaggcg ctggccgggg
    30001 cgcgcgcaga gctcaaggac agcgtcacgc agatggtgcg cgaggcggtc gcggcctcgg
    30061 tcgcgcgcgc gctcgcgatg ccgaaggaat aagtgctcga gaagagcttc aacccggggg
    30121 cgatcgagga aggctggtac cggcgctggg aagacgccgg gtacttccgc cataaggacc
    30181 acgccacccc ggcgtactgc atccagctgc cgccgcccaa cgtcaccggc acgctgcaca
    30241 tggggcacgc gttccagcag acgctgatgg acgcgctcat ccgctaccac cgcatgcgcg
    30301 gcgacaacac caactgggtg gtcggcaccg accacgccgg catcgccacc gagatcgtgg
    30361 tcacgcgcca gatcgaggcc gaaggcaaga ccaagcgcga gctcggccgg gaggagttca
    30421 accggcgcgt atggcagtgg aagcaacaat ccggttcgac catcacgcgc cagatgcggc
    30481 gtctcggcgt ctcggccaac tgggagtacg cggacactga gggccagaag gccggctatt
    30541 tcaccgccga cccgaagatg tcgcgcgcgg tcacagaggt cttcgtgcgc tggtacgagg
    30601 aagggctgat ctaccgcggc cagcgcctgg tgaactggga cccggtgctc ggcaccgggg
    30661 tctcggacct cgaggtcgat accgacgagg aggacggccg catctgggag atccgctacc
    30721 cgggcgaagg attcgacatt cgcgtcgcca cggtgcggcc cgagaccatg ctcggggacg
    30781 tcgcggtcgc cgtccacccg tccgacgcga ggtacgccga tctggtggga aagaaagtgc
    30841 gcctcccgct gaccgaccga accatcccgg tcatcgccga cgagtacgtc gaccccaaat
    30901 tcggcaccgg cgcggtcaag atcaccccgg ggcacgactt caacgactac gaggtcggga
    30961 agcgccaccg cctggagctc atcaatatcc tcacgctgga cgcgcacctg aacgacaagg
    31021 tgccgccgaa atatcgcggc ctggagcgtt tcgcggcgcg caagcaggtg gtagcggacc
    31081 tgaaggccca aggtttcctg gtgagcgaaa aaccctacaa gctgcgcgtg ccgcgctccg
    31141 ggcgcaccgg cgtgatcatc gagccgatgt tgagcgacca gtggttcgtg aagatggaaa
    31201 gccttgccaa ggatgccctc ggggtcgtcg cccgcggcga ggtgaagttc atccccgagc
    31261 actggaccgc cacttacaac cacttcctgg aaaacatcaa ggactggaac atctcgcgcc
    31321 agctctggtg gggccaccag attccggcgt ggtatgacga ggaaggcaag ctctacgtcg
    31381 cacgcagcga ggcggaactt cagcagaaac acccccggaa gaagctgcgg cgcgacccgg
    31441 acgtgttcga cacgtggttc tcctcgcagc tcgtgccgtt cagctcgctc ggctggccgg
    31501 acaagacgcc ggaccaggaa gtcttcctgc cctcgtcggt gctgatcacc ggcaacgaca
    31561 tcatctttct gtgggtgtcg cgcatgatca tgtcgtcgcg ctacttcacc ggcagggttc
    31621 cattccgcga cgtctacatc aacgccatcg tgcgcgatgc cgagggcgag aagatgtcga
    31681 agtcgaaggg caacaccatc gacccgctcg acctgatcga cggcatcagc ctgcaggacc
    31741 tgctgaagaa atccaccatc gggctgatga aggacgagca caaggagcgc atcgagaaat
    31801 acgtgcgcag ccactactcg aacggcatcc cggcgttcgg cgcggatgcc gtgcgcttca
    31861 ccttcgcctc gctcgcgagc ttctcgatca cgctcaattt cgacctcggc cgctgcgagg
    31921 gctaccgcaa cttctgcaac aagctgtgga acgcgacgcg cttcgtgctg atgaataccg
    31981 atggcaagga ctgcggcacg gacgacaagc tgccggtgag gctgtccttc gcggaccgct
    32041 ggatcgtgag tcagctgcag cgcgtggagg cggaagtcga gcatggattt gccgactacc
    32101 gcttcgacaa cgtggcggcg gcgatctacc gctttgtctg ggacgagtac tgcgactggt
    32161 acgtcgagct tgccaagttg cagctgcagg acccggacgc ggccgcccag cgcggcacgc
    32221 gccgcacgct ggtgcgcgtg ctggaggccg cgctgcgcct ggcgcatccg ctgattccat
    32281 tcatcaccga ggagctctgg cagaaactgg cgccgctcgc cggaaagaag ggcgagaccg
    32341 tgatgctgca gcgctacccg aaatcgcaac cggagaagat cgacgaagcg gccgagcgtg
    32401 acgtggcggt gctcaaggag tggacggtcg cggcgcgcaa cctgcgcacc gaggcgaaga
    32461 tcccgcccgg cgagcgggtg atgctttacc gcacggcgga tccggcggtt tccgatccgc
    32521 cggaagtcgc aaaggccatt tcttcgctcg cccggctgag cgggttcgag aagcgcgacc
    32581 ctctgcccga tgcgccctcg ccggtggcgg tggtcggcgg cgcccgcatc atgctctaca
    32641 aggagatcga ccccgccgcc gagcgcgtgc gcctgatgaa ggagattgcg cgcctcgagg
    32701 gagaaatcgg caaggcccgc gcgaagctcg ccaatccttc attcgtggag agagcgcccg
    32761 cgaaagtcgt cgagcaggag cgcacgcgcg tcgcggggtt cgaggcggac ctcgcaaagc
    32821 tcaaagagca gctacggaag ctcggcccct gatctccggc tcggtcggcc aggagtaatc
    32881 gaattcccag ccgtgctgcg cctcgaggcg caggcgaaac gcatgctcca gctcgcccga
    32941 cggcgtcgcc acgcagctgc cggggaagcg catcaggaag ctcgacgcga tgaccgcgca
    33001 ctcgccgagc gcgaggacgc ggctttgcgc gatgtcgcgg ccgtggagga aaagctcgtc
    33061 gttcaggtag gccggcacgc cgaactggcg gtaggtcgcg accgcggcgg cgaggcccca
    33121 ggacagccag cggctggtgc ggcccggggc gaacttgagc ggcgcgatca gcgcgaaggg
    33181 atagcgcgcc gcctcctccg cccataacag cacgggcgcc gcggcgcgcg gctgcgccgc
    33241 ggcgatggaa gcataggccg cgtcgaactc ctgctgcccg atggcgccga gcctgatcca
    33301 gcgcgctttc atgagcacga ttgtttcaca gctcgatgtc ttcgcgaatc gcggcgattc
    33361 cggccgcggc gcaggcgtcg tcctcgcgcg cgccgggcgc gcccgagacg ccgatcccgc
    33421 cgagcagcga gccgcccgcc tcgatcatca tgccgccgcc aaccgcggcc accccgggcc
    33481 ggttgcggat gccgctcgag ggacggccgg gctgcgtggc ttccgcaagc gcggtggtat
    33541 tcgtgcggaa actcaccgcg gtgtaggcct tggcggtcgc catgtcgggc gtgtgcgggc
    33601 cggcgaagcg gtcgcgcagc agcacctgga ccacgcccat gcggtcgacg accgccacgg
    33661 tcgcctggaa gccgcgctcg cggcagctct tgagcgccgc ctgcgccgcc ttcagcgcgg
    33721 tttcaggcgt gagaaccttg accgtgaagg tcgcgtctgc cgcattcacg gccgctgccg
    33781 caagcaaccc gccgagcagc aacgcccggt tcacttcttc ctcatgaaaa aggtgaattc
    33841 cttgttcgcc tcggcgtgcg acacgagctc gtggccggtc tggcgcgaga aggtttgaaa
    33901 gtccttcacg gcgccgggat cggtggcgag caccttgagc acctgccccg agtgcatgtc
    33961 ggtgagcgcc ttcttggtgc gcaggatcgg cagcgggcag ttgagcccgc gggcgtcgag
    34021 ttccttgtcg aagttcatgc aagttattat gcagcatgaa agaagcagac cgcccggcgg
    34081 actgggcctt tgatccgcac gcgacccggc cggtgacgtc cgagccggat ggcgcggatg
    34141 gcgggaagct gccgggatgg ctgctgatga tcgcgcagct tccggtggac gatccgtcct
    34201 cgcgcatgcg cgtgctgcgc acgctggaat cgctcggcgc cgcggtggcg cgcgacggcg
    34261 tctttctgct gcccgacacg ccgtccaacc gccagtcgct cgagacgctg accgagtaca
    34321 tcgccaaggt ggcgggaacc gcgaacgtgc tgcagacgag cgcgctctcg ccggcgcagc
    34381 accaggggtt cgtgcgcctc ttcgaccgct cggcgcgcta cgagaacctg atcaagaccg
    34441 tcgagagcct gcgcgtgggc ttcggccact ccgatccgag cgcgatctcg cgcgtgctgc
    34501 acaagcagcg gcgcgagttc gaggccatcg ccgcgctcga tttcttcccc aacgccgcgc
    34561 gcgagcgcgc cgagcaggca ctcgccgcgg cagacaatga cgtgcgcaag ctgctctttc
    34621 cggcgcaggt gccggtcgcg ctcgcgcccg gccaggggct gctcggccgc acctgggtga
    34681 cgcgcaagcc gctgtgggcc gaccggctcg cctgctcgtg gctcatccgg cgcttcatcg
    34741 accccgaggc gacgctcacc tggctcgaga agacccagtc cctgtcggcc gacgccatcg
    34801 ccttcggctt cgacggcgcg cacttcgcca acagcgagac gcgcgtgacc tacgaggaga
    34861 tgctggtcaa gctcgacctc gccaagaacc cggcgctcgt caagatcggc agcatcgtgc
    34921 acttccttga agtacgcggt acgccggtcg ccgaggccgc gggcgtgcaa acgctgctcc
    34981 agggcgcgca gcgccgctcg aacagcgaac gggagctgct gcaggaagcc gagaagacct
    35041 tcgacctgct gtacgaggcg tactaccagg cgcccaaaaa ttaggaagag cggtaaatac
    35101 taggaaaagc ggcagatcgt cgtccccgcg aaggcgggga cccagtccca attttctaaa
    35161 cggggccccc gccttcgcgg gggcgacaac ctcggacctt ccctagcgct tcgcgtcctt
    35221 catgtcccgc gcgtgctgcg cgcgaagctc cttcagccgc gcgtccacca ccgagttctg
    35281 gtagaagtcg ccgtcgccgg cggcgcgcga gagctggagc tgctcgatgg cggcgggcag
    35341 gctgccctgc agcacgtaca cctcggcctg cgcctggtgc tggagcgagc gcttgccgag
    35401 cgcggcgtag ttcttcgcct gaagctggtg cagccgcgcg tcgcgcggat acaggcgcag
    35461 cgagtcggtg agcagcgtaa gcgcctgctg gttgcggccg gcctcctgca aggcgtcggc
    35521 ttgcgcgtag agtagcgggc gtgaattcgg gaagcgcgca tgcgcctcgc cgagcagcgc
    35581 ggccgcgccg gcgcgatcgc cgagcccgag cctgacgcgc gcggcgaggg tctcgatcat
    35641 cgggccggcg gcgcctgccg cccgcgcccg ctggagctcg gcctgcgctt ctttggggcg
    35701 ccgggcgcgc agcagcgcgc tcgccaggcc gtagcgcgcc gccgactcgc tcgcgtagcg
    35761 cttctcgcgc accgagccct ggaagtactc gacggtgtcg cgcgcgtcgc cggattcggc
    35821 acgcagcttg gcgcgcacca gctgaaactc ggcgctgtcg agatgctgct tgtagggcag
    35881 gctggcggcg cggttctgcg cgtcggcgat ccgctcggtc gtgatcgggt gggtgcgcat
    35941 gtagccgggc atcgagccgt cgtccatggc gcgcgagccg cgctgcatct tctcgaagaa
    36001 ggtccccatc gcgtgcacgt cgaagcccga ggcctcgagc gcctggaagc cgatgcggtc
    36061 ggcctcgcgc tcgtagtcgc gcgagtagct gagctgggtc tggaccgcgc cggccgaggc
    36121 cgcggccgcg gcgccgctcg cgaggtcggg gcgcgagcgc ccgagcagga tcgcggcggc
    36181 gagcgccgcg agcatcggca gctgcatctg ctgctgctgg ctgagcatgc gcgcgatgtg
    36241 gcgctgggtg acgtgcgcga tttcatgcgc cagcaccgag gcggcctcgg actcgctttc
    36301 cgaggccatc aggagcccgg tgtgcacgcc gatgaagccg ccgggaagcg cgaacgcgtt
    36361 gatggtgtgc tcgcggatcg cgaagaactc gaagtcctgt cgcgcgccgg cggcgttctg
    36421 cgcgaggcgc ccgcccaggc tgccgagata gtcggcgatc tcggggtcgt cgagatagtt
    36481 cggctcgcgg aagcggatgt cgcgcatgat gctctcgccg atacggcgtt ccatctgcgg
    36541 cgagagcccg ccatcggcgc cgccgccgag gtccggcaac gcctgcgcga acaccgagca
    36601 ggcgaagaag gcgagagccg caaccccaaa gcgcatgttg tttatgatag cgccccgaat
    36661 gagaagcaaa tcgttgaccc cgcagagact gaccccgcag tcattgaccc acttcgacgc
    36721 caagggttcc gctcacatgg tcgatgtggg cgcaaaggcc gccacccacc gcgtggcgat
    36781 cgcctcgggc cgcatcgtca tgaaaccggc taccctgcgc ctgatcgccg cgggcgccgc
    36841 caagaagggc gacgtcctcg gggtcgcgcg catcgccgcg gtgcaggcgg ccaagcgcgc
    36901 cgccgagctg atcccgctcg cgcacccgat cgcccttacc cgggtcgccg tggatttcac
    36961 tctggacaag agaaccgcct ccgtgcgcat cgtgtcgcgc gtcgaatgcc gcggacagac
    37021 cggggtcgag atggaggcgc tcaccgcggc cgcggtgggg ctgctcacga tctacgacat
    37081 ggtgaaggca gtcgaccgcg gcatgacgct caccgagatc cgtctcgagg aaaaacgcgg
    37141 aggccaatcg ggggtatggc cgcggcgcaa gctaaaatag agtccatgaa caacattcga
    37201 agaaccgttc tcgccgcggt cggcctgctg ctgctcaagc cgctcgccgc ggctgcggcg
    37261 tggaaccagg aagccttcgg cgccaaaagc gccgccgacg ccctgaaagg cctcggcagc
    37321 gcgaaccccg cccccagcaa ggacatcgtg atcgaggcgc cgcagatcgc ggagaatggc
    37381 gcggtggtgc cgatcgagat ttcgagcaac atcccgggca ccacctcgat cgccgtgctg
    37441 atcgagaaga accccttccc gctcaccggc aagttcgagt tcaaggaagg cgcggctccg
    37501 ttcgtgaagc tgaacgtgaa gatgggcgag acctccgatg tgcgcgtggt cgcgatggcg
    37561 ggcggcaagt ccttcgccgc gacgcgcgag atcaaggtca ccatcggcgg atgcggaggc
    37621 tgacatggcg ggaccgatga agatgcgagc gaccctagcc ggcggaaaca ctgccgccgt
    37681 tcataccgag attcgcgtgc tgatgaccca tcccatgacc acggcgcaga tgaaggaagg
    37741 cctgcaccac atccagaacg tggtggtgaa gcagaacggc agggtcgtgg tcgaggccga
    37801 gatcagccag gccgtgtcgc gcaacccggt gttctcgttc cgcctcaagg gcggggccaa
    37861 gggcgacaag atcgaagtga gctggctcga caacaagggc gagtcgaaca agatcgaaac
    37921 tgccgtcgca tgaggtcttg tttcctgctc tgcggagtgc tcgcgtgtac gccgcttttc
    37981 gcgcaggaca ccaagaagga aatccagaag taccagcaga tgatcgccga cggctcgccc
    38041 gtcgagctgt tcgagctcga gggcgaggcc ctctggaaga aggcccaggg gccgaaaaac
    38101 gcttcactcg agaagtgcga cctcggccag ggggcgggcg tgctgaaagg cgcctacgcg
    38161 cgcctgccgc gctacttcaa ggacgccgac cgcgtgatgg acctggagac tcgcctgctc
    38221 cactgcatga ccacgctgca gggccgcagc cgcgaggaag cgaccaagcg cgtcttcggc
    38281 aacgaaaacc agccctcgga gatggagtac ctctcggcct acgtcgccgc ccagtcgcgc
    38341 ggcgccaagc tcgccgccgg cacctcccac ccgagggaga aggacgccta cgagctcggc
    38401 aaggcgctct ttttccaccg cgcgggcggc tgggacatgt cgtgcgcgag ctgccacggc
    38461 gaagaaggca agcgcatccg catgcaggac ctgcccgtgc tctccaagcg cgagttcgcc
    38521 cggccgatca tggccacctg gccggcgtac cgcgtgtcga acagccagtt caagaccatg
    38581 caatggcgca tcaacgactg ctaccgccag atgcgctacc cggagccgag cttcgcctcc
    38641 gacgcgacta tcgcgctcaa caccttcctc acggtcaccg ccgccggcga gccgtatcgc
    38701 ggaccgggta ccaagcgatg acccgggtct tgctttcttc gttcctaatc ttcccgctgc
    38761 ttgctggagc gcagaccacg gcccccgacg tccaaagagt cctgcagcgg gatttcaagg
    38821 cccgcggcca ggcgaccatg gaccgcgtgg tgcaggacgg cgtgcagcgc gtgtgcaccg
    38881 agagcgacga ccggccgccc gccgcgctcg ccaagcagat ggaagccgac cagctgaaga
    38941 ccatcgcctt tcccgagggt agcctgatcg gcgactggaa gcgcggcgag ggcatcgcgc
    39001 agaacggccg cggcctgcag tggaccgatc ctgccggcgc cgggggcggc ggcagctgct
    39061 acaactgcca ccagctctcg ccgcaggaga cctcccacgg cacgctgggg ccgagcctcc
    39121 tacgcttcgg cgcaacccgc ggcaacggcg ccgagatgca gcgctacgtc tacggcaaga
    39181 tctacaacgc caaggcgtac aacctgtgct cgcacatgcc gcgcctcggc tactcgggca
    39241 cgctcactcc cgagcagatc aaggacctcg tcggcctgct gctcgatccc gcctcgccgg
    39301 tcaacaaata gcagtgaaag cccgctggtt ccggccgctc gcgcttctga cggcggcggc
    39361 ggtgatatcg ctgagcgtct tctttctcgc cagagcgaaa tagcttgcaa cgccgggaat
    39421 tccttcagct gctcgccgtg gccgccgccg ccggcgcatc gctgcgtccg cgtactggcg
    39481 aagcccaggc tgctgccgag ctctacgacg tcccgccgtt cggcaacgtc tcgctgctgc
    39541 acatcaccga tacgcatgcc cagctcctgc cggtctactt tcgcgagccg gcgggaaagg
    39601 aatttctgga gaagtacaag acgaggagcg ggacccagtt cgcctatgcc ttcacccgcg
    39661 tcgacttcga gcggctggca cggcgctacg gcaaggtcgg cgggttcgcc catctcgcga
    39721 cgctggtaaa acgcctgcgc gcaacgcgcc cgcatgccct gctgctcgac ggcggggata
    39781 gctggcaggg ctcggccacg gcgttgtgga cgggcggggc cgacatgcgg ggcgcgcagc
    39841 agcgcctcgg cgtggacttc atgactgcgc actgggagtt cacctacggc cagaagccga
    39901 tccaggattc tcccgtcaag gtcctcgcgc agaacgtgcg cacggcagac ttcgaagatc
    39961 cggtgttcga gccgtatgcg ctgcgcagcc tgaacggcat tcccgtcgcg atcatcggcc
    40021 aggccttccc gtacaccccg atcgcgaatc cgcggcactt cgttcccgac tggactttcg
    40081 gcatccagga ggggcggatg cagaaaatcg tcgacgaggc gcgcgcgaag ggagcaaagg
    40141 ccgtggtgct gctgtcccac aacggcatgc atgtcgacct caagctcgca ggccgggtca
    40201 ggggcctgga cgccatcctc ggcggccaca cgcacgacgc cgtgccgcgg ccggtacgcg
    40261 tcggcaggac gctggtcacc aacgcgggct gcaacggaaa gttcctggcg gtgctggacc
    40321 tgcaagtcgg cgcgagcggc gtcaccgact cccgctatcg cctgctgccg gtgttttccc
    40381 ggttgctcga ggaagacaag gacatggccg cctacgtctc gagcgtgcgc gagccctacc
    40441 gggaaaagct cggagagaag ctcgcgctca cggaaggcct gctctaccgc cgcggcagct
    40501 tcaacggcag cttcgacgag ctgatcctgc aggcgtcgct caaggagaag gacgccgaga
    40561 tcgcgttctc gcccggcttc cgctggggca cgaccctgct gccgggcgat gcgatcacgc
    40621 tcgagcacgt catggaccag acggcgatca cgtatcctca atgcacctcg aacgagctca
    40681 gcggcgagca gatcaagcac atcctggagg acatcgcgga caacgtgttc aatgcggatc
    40741 cgtacctgcg gcagggtggc gacatggtca gggccggcgg ggtgcgctat gcgctggaac
    40801 cgggcgcgcg catcggggca cggataacgg acctgcgcat tcgtggaaag cctctggagg
    40861 ctacaaagcg ttacaaactg acagggtggg cgccggtcgc agagggtata acgggggagc
    40921 cgatctggga cgtggtgacg cgccacctgc gcgccgcgaa aaccgtgccg ccgctcgagg
    40981 tgaaccgccc gcggctcatc ggcgttaagg ataatcccgg tctgacatga aacgagctgc
    41041 cgtcctcgct ctggtcgtgc tcgcgatcgc cgccggcggc tggtactggt accagtcgcg
    41101 cgacggcgat gccgagctca ggtaccgcgt cgccaaggtg gagcgcgggt cgctcgcggc
    41161 cgtggtcgtt gcgagcggga cgctgaacgc ggtcaccacg gtccaggtgg gctcgcagat
    41221 ctccgggcag gtgaaggaga tcttcgccga cttcaacacc gccgtgaaga aggaccaggt
    41281 aatcgcccag atcgacccgt cgaccttcga gctgcgcgtg aaccaggcgc gtgccgacct
    41341 cgactcggcg cagggcgcgg tcgcggtggc gcgcgccggc atggcggcgc agcaggccga
    41401 ggtcgcgcga gtcaaggtga cgctcgccga agcgcggcgc gacgtcgagc gcaaaaaggc
    41461 gctggtcgac aagaagttca tctcggcggc cgagctcgac aaggcggtgg tggtggtcga
    41521 caccacgcgc gagcagctga acgcggtgca ggcgcagatc aaggtgggcg aggcccaggt
    41581 ctccagcgcg cgcgccgccg tcaagcagcg cgagtcgctg ctcaagcagg cgcaagtgga
    41641 cctcgagcgc accatcatcc gcgccccggt cgacggcacg gtgatcctgc gcaacgtcga
    41701 tgccggccag acggtcgcgg cgagcttgca agcgccggtg ctcttcacca tcgcccagga
    41761 tctgcgcgac atgcaggtcg aggcggcgat cgacgaggcc gacgtcggcc ggctgcgcgt
    41821 cggcatgccg gcgggctttg cggtcgacgc cttcccgcgg cgcaacttcg cgggcgagat
    41881 caagcagatc cgaaagtcgc cggtcaacgt gcagaacgtg gtgagctata ccgtggtgat
    41941 ctcggcggcg aacccggacc tcgcgctcct tcccggcatg accgccaacg tgcgcatcgc
    42001 ggtggagaac cgcgccaacg cgctcaaggt gccgaatgcc gcgttgaggt ttcgccctgc
    42061 gggaaccgtc gatgtaaagg cgccagccga cgcgccgccg cagcagcagg gcggccaggc
    42121 cgtgcaggaa ttccgcaacc gcatccagca ggagctcaag ccgagcgacg cgcagcgcgc
    42181 ccagctcgag gagatcttca ccgagtcgcg ccagaagttc gcccgcgtgc gcgacatgaa
    42241 aggcgacggc aaccgccgca aggaagccga gcgcatccgc gccgagacca atggccgcat
    42301 cgcggagatc ctgacacccg agcagcgccc cgcctgggag cggctgctgg ccgaggccgg
    42361 ctcgcgcggc ccgtcgacca gcggccgggt gtacgtgctg gagaacgccg agcccaagcc
    42421 gctcgacgtg cgcctcgggc tctccgatgg catgagcacc gaggtcctga gcggactcgc
    42481 cgaaggcact gaggtgatca tcggcacggc cgacgcgcgc ggttctgcgc cggcggccgg
    42541 cggtgggctg ccgcgcgcgc ggttcttctg atgctgattc atacccgcga cctcgtgaag
    42601 gtctaccgca tggggggcgg ggaggtgcgc gccctcgacg gcgtgagcgt cgacatcgcc
    42661 gaaggcgagt tcgtcgccgt gatgggcccc tcgggctccg gcaagtcgac cttcatgaac
    42721 gtgctcggct gcctggatcg tccgaccagc ggggagtacg tgctggggga caaacgcgtc
    42781 tccgagctcg cgggcgacga gctcgccatc gtgcgcaacc gcaggatcgg gttcgtgttc
    42841 cagcagttca acctgctcgc gcgcacgccg gcgtccgaga acgtcgagct gccgctggtg
    42901 tacgcgggct tgcccaagaa gcaaaggctc gagaaggcca aggcgatgct cgcgcgcgtt
    42961 gggctggccg accgcggcca ccaccacccg gcgcagctct cgggcggaca gcagcagcgg
    43021 gtcgcgatcg cccgggcgct cgtcaccgag ccgctcctga tcctcgccga cgagccgacc
    43081 ggcgcgctcg attcgcgcac ctcgctcgag atcatggcgc tgttccagga gctgaaccgc
    43141 cagggaatga cggtggtgct ggtgacgcac gagcccgacg tggcgcgctt cgcccggcgc
    43201 atcatcgcct tccgggacgg caaggtcgtc caggacgagc gcaacgaagc cctcgccgca
    43261 tgagcttcgt cgcgctgctc cgcatcgccc tgcgcgcgct cgcggtaaac aagctgcgca
    43321 gcgcgctcac catgctcggc atcgtgatcg gcgtcggcgc ggtgatcgtg atgatcgcgg
    43381 tcggcggcgg cgcgcaggcg cgcgtggaag agcagatacg ggccctcggg tcgaatctcc
    43441 tgctcgtcat gccgggctcg accaccgccg gcggcgtgcg catgggcttc ggctcgggct
    43501 cgacgctcac cgaggacgac gtcggcgcga tcaaccgcga gatccccgag gcgctcggcg
    43561 cgccggcgct gcgcggctcc gcgcaggtga tctggggcaa cgccaactgg tcgacgcaga
    43621 tcttcggcgt cacccccgag tacctcgacg tgcgccagtg gccgctcgcg tccgggcgca
    43681 gcttcgagcc cggcgagatg gccggggcgg ccaaggtctg cctgatcggc gccaccgtcg
    43741 cgcgccagct cttcggcgcc gccgatccgc tcgaccagtc gatccgcatc aagcgcgtgc
    43801 cgttcaccgt aatcggcgtg ctcgacgtca agggacagag cctgatggga accgaccagg
    43861 acgacctggt gctggtgccg atcaagaccg cgcgcagccg cgtgctgggc accgcgagcg
    43921 cggcgcgcaa ccgcaccgtg ggcacgatct gggtgaaggt cgccgacggc ctcgacacca
    43981 aggtggtcga ggagcaggtg cgctcgctcc tgcgccagcg ccaccgcatc caggccggcg
    44041 ccgacgacga tttctcgctg cgcaacctcg ccgaggtgat ggcggcgcag gaggcgtcga
    44101 gccgcgtgct cgcgctgctg ctcgccgccg tggcctcggt ttcgctcctc gtgggcggca
    44161 tcggcatcat gaacatcatg ctggtgtcgg tgaccgagcg cacccgcgag atcgggctgc
    44221 gcatggcggt gggcgcgcgc acgcgcgaca tcctcggcca gttcctggtc gaagcggtga
    44281 cgctgtcgct gatcggcggc ctgatcggcg tcgcgctcgg catcggcggg tcggtggcga
    44341 tcgcgcagct cgccggctgg cgcatcgtgc tctcgcccga ggcggtcggc ctggcggtcg
    44401 cgttcgcctt cgccatcggc gtgttcttcg gcttctatcc tgcgcgcaaa gcggcgcggc
    44461 tgaaccccgt cgaggcgctg cgcttcgagt gagctagacc ttctcgacga ccagcatctt
    44521 gaccggctcg gcggtcaggc ggtcgatcaa taccgccacc caggcgttgc gcccgcgcag
    44581 ccgcacgtag cgcaggcgct gctcctccac gccggagcgc gcgagccacg cgtcgatcac
    44641 ttttgcgacg acgggctcga ccctgcgcac ctccgacaaa ggctgtgccc tggcgagaat
    44701 ctggtccctg ccttgctcgt acggcgcgta gtactgggga aaatgctgca ggtcctcccc
    44761 gccgagcgac gcgaacagca gctgctgctg ctcggcgaaa tccttcggcc agtcgccgaa
    44821 cacgagcacc ggccccgacc acgacgggct gcggtactgc ggatacctcg ccgtggcgag
    44881 ccgctccggc ggaagctcga ccacggtggc gatctggaac tggtccttga cgaagacgat
    44941 gaacgcgggg cgcgccaggg aaatgatatg gcagccgtac accagggcgg caagctggaa
    45001 caccgcgatc gccgcgagat cgaacttcag gccgggcttg ccgctgcgga acacgacgag
    45061 cgtcagcgcc ggcccgatca ccacgtcgac gccgaccagg atcagcgcga gcccgctgcc
    45121 gcccatggcc tcgaacagcg gcggcgggta ccagagcgcg agcatcacgc cgagcacagc
    45181 ggcggcgacg caggcgctga tcagcaggtg cgcacccgcg gcgcgccagc gcgtaaacgg
    45241 ccgcttcagt gcggtctcgc ttttcttcat ccgctcggtc gcctatctta gcaacaacgc
    45301 tcggctacaa tgcgccgctc tcatggcgcg ggctacatcc tgagatgaac tgcctcgtcc
    45361 tgggaggagg cgggttcatc ggctcgcacc tggcgctggc gctcgccgac gccgggcacc
    45421 gcgtgcgggt gttcgaccgg ccgcaggggc gcttcctgcc gctgctcgag gggtgcgaag
    45481 tgttcacggg cgactttctc gagccgcgcg atctcgagcg agcgctgagc ggcaccgagg
    45541 tcgtgtgcca tctcatcgcc accacggtgc ccaagagctc gaacgaggac caggtctccg
    45601 acgtcgccgc caacgtcgtc ggcacgctgc gcctgctcga gctgtgccgc agccgcgggg
    45661 tgcgccggat cgtgttcccg tcctcgggcg ggacggtcta cggcatcccg cgcaccacgc
    45721 cgatcggcga gacgcatccc accgacccga tcagctccta cggcatccac aagctgacga
    45781 tcgagaaata cctgcacctg aaccaggtcc tgaacgggct cgactaccgg gtcctgcgca
    45841 tcgccaacgc gtacggcgag cgccagcgca ccgagacggc gcagggcgcg gtgtcggtgt
    45901 tcctcgagcg cagcctgcgc ggcgacgcgc tgcagatctg gggcgacggg tcggtggtac
    45961 gcgactacat ctacgtcggc gacgtggtcc aggcgttcct gaggatcctc gcctacgagg
    46021 gcgagcaccg gatcttcaac gtgagctccg gggtcggcgt gagcctgaac cagctggtgg
    46081 acgagatcgc gcgcgcgatc ggccgccgcc ccgcggtcga atatgcgccg gcgcgccgct
    46141 tcgacgtgcc ggtcaacgtg ctcgatgcct cgctcgcgcg caagcacctc ggctggagcg
    46201 cggccacgcc gctcgccgag ggcctgcggc gcacctacga ctggatgcgg cggtgattcg
    46261 cgccgagcgc aatctcttcc tgatcagctt cctgatcctg ttcttcgagc tggcggcgat
    46321 ccgctggttc gcggcgacgg tggtgttcct cacgttcttc accaacatcg tgctgctcgc
    46381 ctgctttctc ggcatgagcg tggggctgct ggccgcgcgc cggccccaga acctggtcca
    46441 ggccgcgctg cccatcgcca tgctcgcctt cgccgcggcg gttgccacgc atctcgccta
    46501 cctgaactgg gccgagcagc tgacggtcag cgtcagcgac caggcgtcgc cgcgcatgat
    46561 ctatttcggc accgaatacc ggcccgccga cccgagccgc tggatcgtgc cgatgtgggc
    46621 ggtcggcggc gcgttcttca cgctcctggc gctgaccttc gtcggcctgg ggcaggtcat
    46681 ggggcgcgcg ttcgacgcca tccccgaccg tgtgcgcgcc tacaccatcg acgtcctggg
    46741 cagtctcgcc ggcatcgccg cgttcgcggc catgagttac ctggagcttc ctccgacggt
    46801 gtggttcgtc cccatcctgc tgctgatgct ctatttcgcc ggctggcgca agccggtgca
    46861 gctcaccgct gccgcggtgg tctacgcgat ggtcggcttg agcgcgcacg tgctgaccgc
    46921 ccagggcacc cagattttct ggtcgcccta ttacaaggtg tcctacgatc cgccgttcca
    46981 ggtcatttcg accaacgaca tcgggcacca gaacatggtg gccgtcgggc agtccgggcc
    47041 ggcctacctg ctgccgcacc tcctcaaccg ggacgccggc ggcgcgccct tcggcgaagt
    47101 catgatcatc ggcgccggca gcggcaacga cgtcgccagc gcgctgcgct ccggcgcaag
    47161 gcacgtcgac gccgtcgaga tcgatccgcg catcaaccgg ctcggccgcg agctgcaccc
    47221 cgagcggccc tatgccgacc cgagggtgag cgtgcatctc gacgacgggc gcagcttcac
    47281 gcggcgtacc gagcggaagt acgacctcgc ggtgtacgcc gtcgtcgact cgctcgtgct
    47341 gcactccggg tattcgagcg tccggctgga aaacttcctc ttcacgcgcg aggcgttcga
    47401 ggatgtgaag cgcgtgctca agcccgacgg cgtgttcgtc atgtacaact tctatcgcca
    47461 gggctggctg gccgcaaggc tgaaacgcat ggcccaggag gcgttcggcg tggagccggt
    47521 cgtggtctcg ctgccgcacc gggaccacat ccccgacgac gacagcgttc tgcacgggtt
    47581 caccttcctc ctggtgagcg ccgatcccgg gcgcctggcg gcgctgcgcg cgcgcttcga
    47641 gaagggcgag agcttctgga tccaccccta tccgccgatg aacgaggcgg tcaacggctt
    47701 ccgcgccgag gcgcccggac cgaagtggca ccgcatcggg cctacccagg tggacccgag
    47761 cgtcgacgga cgggtgccga gcgacgactg gccgcaggcc tacctgcgcc ggccggagat
    47821 tccctgggcg ccgatcggcc agggcatggc ggcgatcgcc gtgatctcgg ctgtgatcct
    47881 gttcgcgttc gcccccgcgc ggcgcatgcg cccgaactgg cagatgttct tcctcggcgc
    47941 ggggttcatg ctgctcgaga ccaagggcgt ggtgcacatg gcgctgctct tcggcagcac
    48001 ctgggtggtc aactcggtgg tgttcttcgc gatcctggtc atgatcctgg ccgcgaacct
    48061 ttatgtcgtg aagataagac ccaataaatt gaccgtctac tacgcccttc tcgtgctcgc
    48121 cctgcttgtc aatgcgctgg tccccatgaa cgtgttcctg tcgctttcgc cgctgtggcg
    48181 cacgctcgcc tcgtgcgtgg tggtgttcgc gccggtgttc ttcgccggcg tgatcttcgc
    48241 caccgccttc cgcgagagcc ggcagccgga cgtcgatttc ggctggaacg tggccggcat
    48301 cgtcctcggc ggcctgagcg agcagctgtc gctcgtgctc gg
//
LOCUS       DATBSZ010000293         5905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1356452, whole genome shotgun sequence.
ACCESSION   DATBSZ010000293 DATBSZ010000000
VERSION     DATBSZ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356452"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1919)
                     /locus_tag="VIG70_19265"
     CDS             complement(<1..1919)
                     /locus_tag="VIG70_19265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8252735.1"
                     /translation="MNDALSRAALAVSAAEGGGVFDRLACGVAEILGVDIGFIAVFAD
                     PARSEMRMLAFSLDGRIRPPFTYALEGTPCAAVVGREFRCLPAGARKEFPENDLLGKL
                     GLESYAAYPLNDAAGTPLGLIGAMHRKRLRNVALCEAILKIFALRAAGELEHGKRKDT
                     LEQLRASEAQYRAIFNAAADSMVLRDSEFRVVDVNPAYEKMSGRPREEALGRSDLTMS
                     PPELTERVRAMHARALAGEPVMFEALARRKDGSRFDIETRGVPILHEGRPHVLYIGRD
                     ITARKRSEELLRTSEEQYRAVFNAAADALVLRDADFRIVDVNPAYEAMSGYARDEVIG
                     LDRVVANPPEMYQQLRALHTRALAGETVMVETVRVRKDGTRRDVELRGVAIQFRGKPH
                     VLYIGRDISERKRGEAELRASEEQYRSIFNAAADALVLRDAETRVVDVNPAFLEISGY
                     TRDEVISANRWIFASPSQFALAKEMHRRVIAGESVQFEVDGQRKDGSRFVVEMRAVPI
                     VYRGEPHALGMARDITQRKALESRLRQAQRMEALGHLTGGVAHDFNNLLAAIMGYIVL
                     AEEREGARSPAAADPKLGAYLAQALASSRQARDLVQQMLTFSRGQRGAPKAMNLAAGV
                     EGSLTLMRGSLPATL"
     gene            complement(1995..2702)
                     /locus_tag="VIG70_19270"
     CDS             complement(1995..2702)
                     /locus_tag="VIG70_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8252736.1"
                     /translation="MGATRVLVVDDDPAVRQMLAEYLGAHGYEVRQAASGQAMRAELE
                     RAAPAIVLLDIGLPGEDGLTLARYLRERHDVGIIMVTGAGDVVDRVAGLEVGADDYIA
                     KPFDPRELRARLKSVLRRLEGKAEKKSSSTNKVSVGRCLLDLKSRTLSDAKGREIAIT
                     AMEFELLKALIDHPNQVLSRDRLLTLTRNREWEPFDRSIDIRITRLRRKVEDDPAHPR
                     AIRTVRGAGYMFIPADK"
     gene            2809..2985
                     /locus_tag="VIG70_19275"
     CDS             2809..2985
                     /locus_tag="VIG70_19275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252737.1"
                     /translation="MKRMFRLAVDAFMAVRMQRAHAKLVEQLDERTLRDIGMDLEANR
                     ARERNRLAVRFGGY"
     gene            2985..3305
                     /locus_tag="VIG70_19280"
     CDS             2985..3305
                     /locus_tag="VIG70_19280"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEY8252738.1"
                     /translation="MNHEYAALGAVSMTRGSILRIEDGRDRLIYVWEGELWLTQEGDG
                     RDRCLGPGSWFRLDRKGVAVAQAIRRSTVTLSAKAGEPLGARLTRFWTGLFAPHARPT
                     TAAL"
     gene            3302..3745
                     /locus_tag="VIG70_19285"
     CDS             3302..3745
                     /locus_tag="VIG70_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HEY8252739.1"
                     /translation="MTPRQIALVQESWKRVLPIGDQAAELLYARLFALDPSLERMFRG
                     DMRVQGRKLMAMVSAAVSSLTRLDSIVPVVQALGRRHAGYGVEERHYAVVEEALVWTL
                     EQGLGAGFTKEVEEAWRNAYGLLATTMKTAANQPEASHAAELAAS"
     gene            3720..4658
                     /locus_tag="VIG70_19290"
     CDS             3720..4658
                     /locus_tag="VIG70_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252740.1"
                     /translation="MQPSSQHPDTHRYAKSIEVSKRVRFDIERDVIRGRTLDVTKKFL
                     PDGLSKVDRLEFLSGAQARLMSQIQGRTYANMFGLVERFIGPKVSEISRDHWLGDQTA
                     FEALVRFTEEELKHQELFRRMERLAGQDMHAGYRFLPQPNEVARAVLGKSTWAVLALI
                     CHIELFVLAHWREAIESDPALSELWKDVFMFHAREESQHAILDELEWKREDAKLGPAE
                     VDQGVSDLIDLVAAVDGILQMQAKADSDYFLQSCGTTFDEVQTRRIRDTMLAAYRWQY
                     IASGVQDERFQKILGGMVTDAQMARIGAALKPILES"
     gene            4658..5893
                     /locus_tag="VIG70_19295"
     CDS             4658..5893
                     /locus_tag="VIG70_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8252741.1"
                     /translation="MLDLRECTVSGASPRALELYERSLASLWLQRSDSLELARQAAAE
                     APAFVAAHLLEANLLACSRDPRDFEAAGWAFAKLRGLAMNPREAAHRAALAAAIDGDY
                     ARACRLYERILEEYPRDALALWAALTMDYYLGDSQALRDRPARVLPHWSASVPGYHAV
                     VAMHAFGLAECGDYAAAEDAALRALELEPQDVRAEHALLHVLEMQGRALEGLRRAVNH
                     PVNHIWWHRALFYLQLDLPEEALALYDRRLRFDSLPDLIDASALLWRLRLAGVKVGER
                     FVPLAARWSQHAEDAYCAFNDLHAMMAFAGAQRWDWAQRLLAAQERRIARAAGTNHDM
                     TRLVGYPACRALAAFGRNDFRGADVLLRSLPPVAHRIGGSHAQRDILMLTRAAALRRL
                     QGFQGDFRASFASRRLAAA"
BASE COUNT          875 a         2053 c         2067 g          910 t
ORIGIN      
        1 tcgagcgtcg ccggcagcga gccccgcatc agcgtcagcg agccctcgac cccggccgcc
       61 aggttcatcg ccttcggcgc gccgcgctgg ccgcggctga aggtcagcat ctgctgcacc
      121 agatcgcgcg cctggcgcga cgaggcgagc gcctgcgcga ggtaggcccc gagcttcggg
      181 tccgcggcgg cgggcgagcg cgcgccttcc ctctcctcgg cgagcacgat gtagcccatg
      241 atcgcggcga gcaggttgtt gaagtcgtgc gccacgccgc cggtgaggtg gccgagcgcc
      301 tccatgcgct gcgcctggcg caggcgcgat tccagcgcct tgcgctgcgt gatgtcgcgc
      361 gccatgccga gcgcgtgcgg ctcgccgcgg tagacgatcg gcaccgcgcg catctcgacc
      421 acgaagcgcg agccgtcctt gcgctgcccg tcgacctcga actgcaccga ctcgcctgcg
      481 atcacccgcc ggtgcatttc cttggcgagc gcgaactgcg aagggctggc gaagatccag
      541 cggttcgcgc tgatgacctc gtcgcgcgta tagccgctga tctcgagaaa cgccgggttg
      601 acgtcgacca cgcgcgtctc ggcgtcgcgc agcacgagcg catccgcggc ggcgttgaag
      661 atcgagcggt actgctcctc gctcgcccgc agctcggcct cgccgcgctt gcgctcgctg
      721 atgtcgcggc cgatgtagag cacgtgcggc ttgccgcgga actggatcgc gacgccgcgc
      781 agctccacgt cgcggcgcgt gccgtccttg cgcacgcgca cggtctccac catcaccgtt
      841 tcgcccgcca gggccctcgt gtgcagcgcc cggagctgct ggtacatctc gggcgggttg
      901 gcgaccacgc ggtcgaggcc gatcacctcg tcgcgggcat agccgctcat cgcctcgtag
      961 gccgggttca cgtccacgat gcggaagtcg gcgtcgcgca gcaccagcgc gtcggccgcg
     1021 gcattgaaca cggcccggta ctgctcctcg ctcgtgcgca gcagctcctc ggagcgcttg
     1081 cgcgcggtga tgtcgcggcc gatgtagagc acgtgcggcc gtccctcgtg caggatcggc
     1141 acgccgcgcg tctcgatgtc gaagcgcgag ccgtccttgc gccgcgcgag cgcctcgaac
     1201 atcaccggct cgccggcgag cgcgcgcgca tgcatggcgc gcacgcgctc ggtcagctcg
     1261 ggcgggctca tggtgaggtc gctcctgccc agcgcctcct cgcgcggccg cccgctcatc
     1321 ttctcgtagg cggggttgac gtcgacgacg cggaactcgg agtcgcgcag caccatcgag
     1381 tcggccgcgg cgttgaagat ggcgcggtat tgcgcctcgc tcgcgcgcag ctgctccagc
     1441 gtgtccttgc gcttgccgtg ctcgagctcg ccggcggcgc gcagggcgaa gatcttgagg
     1501 atcgcctcgc acagcgcgac gttacgcagg cgcttgcggt gcatcgcgcc gatcaggccg
     1561 agcggcgtgc cggcggcgtc gttcagcgga taggccgcgt agctctccag gccgagcttg
     1621 ccgaggaggt cgttctccgg gaattccttg cgcgcgcccg ccggcaggca gcggaattcg
     1681 cgcccgacca cggccgcgca aggggtgcct tccagggcgt aggtgaacgg cgggcggatg
     1741 cggccgtcga gcgagaaggc aagcatgcgc atctccgagc gcgccgggtc agcgaacacg
     1801 gcgatgaagc cgatgtcgac gccgagaatc tcggcgaccc cgcaggccag ccggtcgaac
     1861 acgccgccac cctcggcggc cgacaccgct agtgccgctc ggctaagggc gtcgttcatg
     1921 taaaggcagt gtaatcgcgg cgccgcccgg cggcagcccc ctgttacagg acagaaacga
     1981 ttgaaaccgg gcggttactt gtcggctggg atgaacatgt agccggcgcc ccggaccgtg
     2041 cggatggcgc gcggatgggc ggggtcgtcc tcgaccttgc gccgcagccg ggtgatgcgg
     2101 atgtcgatcg agcggtcgaa cggctcccac tcgcggttgc gcgtcagcgt caacagccgg
     2161 tcgcgcgaca gcacctggtt cgggtggtcg atcagcgcct tgaggagctc gaactccatc
     2221 gccgtgatcg cgatctcgcg gcctttggcg tcgctcagcg tgcgcgattt caggtcgaga
     2281 aggcagcggc ccacggagac tttgttcgtt gaggaggact tcttctcggc tttgccttcc
     2341 aggcgccgca atacgctctt cagccgggcg cgcagctcgc gcgggtcgaa cggcttggcg
     2401 atgtaatcgt cggcgcccac ttccagcccc gcgacgcggt cgaccacgtc gcctgcgccg
     2461 gtgaccatga tgatgccgac gtcgtggcgt tcgcgcaggt agcgcgcgag ggtcaggccg
     2521 tcctctcccg gcagcccgat gtcgagcagc acgatcgccg gcgcggcgcg ctcgagctcg
     2581 gcgcgcatcg cttggccgct cgccgcctgg cgcacttcgt agccgtgtgc gccaaggtac
     2641 tcggccagca tctggcgcac tgccgggtcg tcgtcgacaa cgaggacgcg ggttgctccc
     2701 atgtgcccag tatacaaggc cgaaacaaac tgtttcccaa acgaaatcgc ggcgataccc
     2761 gcgcgcccta cgctgccgcc atcgcaacta ccgttaggga gaagcaccgt gaagagaatg
     2821 ttcaggctcg ctgtcgatgc tttcatggcc gtgcggatgc agcgcgcgca cgccaagctg
     2881 gtggagcagc tcgacgagcg caccctgcgc gacatcggca tggacctcga ggcgaaccgc
     2941 gctcgcgagc gcaaccggct tgccgtgcgt ttcggggggt actgatgaac catgagtacg
     3001 cggcgctcgg cgccgtctcc atgacgcgcg gcagcatcct gcgcatcgag gacggccggg
     3061 accgcctgat ctacgtctgg gaaggcgagc tctggctcac gcaggagggc gatgggcgcg
     3121 accgctgcct cggccccggc agctggttcc ggctcgaccg caagggcgtc gcggtcgcgc
     3181 aagcgattcg gcgcagcacc gtcaccctga gcgccaaggc aggcgagccg ctcggcgcgc
     3241 ggctcacccg gttctggacc ggactgttcg cgccgcacgc gcggccgacc acggcggcgc
     3301 tatgacaccc cgacagatcg cgctggtgca ggagagctgg aagcgcgtcc tgccgatcgg
     3361 cgaccaggca gcggagctcc tgtacgcgcg cctgttcgcg ctcgacccgt cgctcgagcg
     3421 gatgttcagg ggagacatgc gggtgcaggg acgcaagctc atggcgatgg tctcggccgc
     3481 ggtgagctcg ctcacgcggc tcgacagcat cgtcccggtc gtgcaggcgc tcggccggcg
     3541 tcacgccggt tacggcgtcg aggagcggca ctacgccgtc gtcgaggaag cgctggtgtg
     3601 gacgctggag cagggcctgg gcgccggatt cacgaaggaa gtggaggaag cgtggcgcaa
     3661 cgcgtacggc ctgctcgcga ccacgatgaa gaccgcagca aaccaaccgg aggcaagcca
     3721 tgcagccgag ctcgcagcat cctgataccc accgctacgc gaagtccatc gaggtctcca
     3781 agcgcgtgcg cttcgacatc gagcgcgacg tgatccgcgg acgcacgctc gacgtgacga
     3841 agaagttcct gcccgacggg ctctccaagg tcgatcggct tgaattcctg tccggggcgc
     3901 aagcgcgcct gatgagccag atccagggcc gcacctacgc caacatgttc ggcctggtcg
     3961 agcgcttcat cgggcccaag gtgagcgaga tcagccgtga ccactggctc ggcgaccaga
     4021 ccgcgttcga ggcgctggtg cgctttaccg aggaggagct caagcaccag gagcttttcc
     4081 ggcgcatgga acgcctcgcg gggcaggaca tgcacgccgg ctaccgcttc ctgccgcagc
     4141 cgaacgaggt cgcgcgcgcg gtgcttggca agtcgacctg ggcggtgctc gcgctgatct
     4201 gccatatcga gctgttcgtg ctcgcgcact ggcgagaagc gatcgagtcc gacccggcgc
     4261 tctccgagct gtggaaggac gtcttcatgt tccacgcgcg cgaggagtcg cagcacgcga
     4321 tcctcgatga gctcgaatgg aaacgcgagg acgccaagct cgggccggcc gaggtcgacc
     4381 agggtgtgag cgacctcatc gacctcgtcg ccgcggtaga cggcatcctg cagatgcagg
     4441 ccaaggccga cagcgactac ttcctgcagt cctgcggcac gaccttcgac gaggtccaga
     4501 cccgaaggat ccgcgacacg atgctggcgg cctaccgctg gcagtacatc gcctcaggcg
     4561 tgcaggacga gcgcttccag aagatcctcg gcggcatggt caccgacgcg cagatggcgc
     4621 gcatcggcgc cgcgctcaag ccgatcctgg agagctgatg ctcgacctgc gcgaatgcac
     4681 ggtcagcggc gcctcgccgc gggccctcga gctctacgag cgctcgctgg cgagcctgtg
     4741 gctgcagcgc agcgactcgc tggagctcgc gcggcaggcg gcggcggagg cgccggcgtt
     4801 cgtcgccgcg cacctgctcg aagcgaacct gctcgcctgc agtcgcgatc cgcgcgactt
     4861 cgaggccgcg ggctgggcgt tcgcaaaatt gcgcgggctg gcgatgaacc cgcgcgaagc
     4921 ggcgcaccgc gcagcgcttg ccgccgcgat cgacggcgac tacgcgcgcg cctgccggct
     4981 ctacgagcgc attctcgagg aatacccgcg ggacgcgctc gccctctggg cggcgctgac
     5041 gatggactat tacctcggcg actcgcaggc gctgcgcgat cggcccgccc gcgtcctgcc
     5101 gcactggtct gcctcggttc ccggctatca cgcggtggtc gcgatgcacg ctttcgggct
     5161 cgcggaatgc ggcgactacg cggcggccga agacgcggcg ctgcgcgcgc tcgagctcga
     5221 gccccaggat gtccgcgcgg agcacgcgct gctgcacgtc ctcgagatgc agggaagggc
     5281 attggaaggc ctgcgccgcg cagtcaacca tcccgttaac cacatctggt ggcaccgggc
     5341 gctcttctac ctgcagctcg accttccgga agaggcgctc gcgctgtacg accgccgcct
     5401 gcggttcgac agcctgccgg acctgatcga cgcttcggcg ctgctctggc ggctgcggct
     5461 cgccggggtc aaggtcggcg agcgcttcgt gccgttggcg gcgcgctggt cgcagcatgc
     5521 cgaggacgcg tactgcgcgt tcaacgacct gcacgcgatg atggcgttcg ccggcgcgca
     5581 gcgctgggac tgggcgcagc gcctgctcgc ggcgcaggag cggcgcatcg cccgcgcggc
     5641 cggcaccaat cacgacatga cccggctggt gggctacccg gcgtgccggg cgctcgctgc
     5701 attcgggcgc aacgatttca ggggcgcgga cgtgctgctg cgttcccttc ccccggtcgc
     5761 gcaccgcatc ggcggcagcc atgcgcagcg cgacatcctg atgctcaccc gggcggccgc
     5821 gctgcgccgc ctgcaaggat ttcaaggaga ctttcgtgcg tccttcgcgt cgcgacgcct
     5881 tgccgcagct tgacggcggc ctgtt
//
LOCUS       DATBSZ010000294         3957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1563037, whole genome shotgun sequence.
ACCESSION   DATBSZ010000294 DATBSZ010000000
VERSION     DATBSZ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563037"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VIG70_19300"
     CDS             complement(<1..925)
                     /locus_tag="VIG70_19300"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8252742.1"
                     /translation="MAPISRLVVAALAAALLLAGCAAPLAPAAAEKPRLVVFLVVDGL
                     PQRQVVDYRDQLAPDGLRRFLERGAWFADAHYAHSNTQTAPGHATMLTGAYPHRSGII
                     ANEWRDPASGELEYNTGDPAASYIGHKTGRLDGTSPKNLRAETVGDVLRRLDARSKVI
                     GISGKDRGAILPAGHRGTAYMYQAQTGQFASTTYYMKAHPQWVVDFHARKPADAYFQK
                     EWQPLLDAPAYARSLPDGQNWYAKWGGKLPMKLGEGMETPGPRFYASVLASPFGDELM
                     LAFARAAIAGEELGKDDAPDILSVSLSSHDYI"
     gene            979..2475
                     /locus_tag="VIG70_19305"
     CDS             979..2475
                     /locus_tag="VIG70_19305"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY8252743.1"
                     /translation="MIDALVQGLLLVLQWNAFSLMLIGMAIGFVVGLLPGIGGAATLA
                     LMLPFVFKMSPVEAFAFLLGMHSVAATTGDITSILFGVPGEGLSAATVVDGHPMAKRG
                     EAARALGAALMSSLVGALIGAAALAVSIPIVRPLVLTFGSPELFMVTVMGIACITSLS
                     GQGTRAQIKGFAMGFLGLLLATIGQERQSGILRFDFGLIYLWEGLDLIPVLVGIFAIP
                     EIIDLAVRGTSIAGNAPAAKLGAGVWEGIKDTFRHFWLVVRCSMVGVFVGILPGAGGG
                     VAQWMAYAHAAQSAKDATDRSRFGTGDVRGVLGPGAANNSKEGGDLIPTIAFGVPGSG
                     AMAILLGAFLIMGLHPGPDMLTKHLAVTYSMVWTLVIANVITVIACLALLPYLAYVTR
                     IRGSVIIPFLLLLVFVGSYTANKQIADLLVTLAAGALGYLMVRFEWPRAPLVLGFVLG
                     KLAETYLFISMARYGYSWLGQPLVIVLMVLTAAVVVYPYFQDRRIRAA"
     gene            2465..2902
                     /locus_tag="VIG70_19310"
     CDS             2465..2902
                     /locus_tag="VIG70_19310"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY8252744.1"
                     /translation="MRLSGAAVFSFGLAAAAAYAVFAALRWPPKAALFPLTMGIPLLV
                     LAVAQTVVELRGPSGQSNPDGATRRAFAVFAWMASFIILVLLAGFPIAVPVFVFLYLV
                     MESREKLVLSIVLAAAAWGGFYLLFQRLLHFPFEDGLIRGWFQ"
     gene            2914..3912
                     /locus_tag="VIG70_19315"
     CDS             2914..3912
                     /locus_tag="VIG70_19315"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY8252745.1"
                     /translation="MKARLIAAIAALTVTAPAWAQGSFYEGKTVTVVIGASGGSLEIA
                     ARIVARHLGKHIPGNPSVIVQNMTGAAHLVATNHVYNVAKPDGLTLLAANPNVGIAQL
                     TKVEAARFDARRFQWLGSSGADGVALSIRADLPYKSWDEVKKSGKELVVGTTGPGSNA
                     HDFPLLLKEFAGANFKLVSGYPANADVLLAIERKEVDVWAAFATTIKLAADRGAVRPL
                     VRGRVALPGWEHLPVDEELATSAVGKAIMGIRAAPLAIGRAVAAPPGTPVDRVQVLRE
                     AFAKAVKDPEFAAESKKAKIEIGYIGPEEVLKMFNGLLDQPAEVQKEMVKYIKFGS"
BASE COUNT          578 a         1327 c         1351 g          701 t
ORIGIN      
        1 tgatgtaatc gtggctcgac aggctgaccg agaggatgtc gggcgcgtcg tccttgccga
       61 gttcctcgcc ggctatcgcc gcccgggcaa aggcgagcat cagctcgtcg ccgaaggggc
      121 tggcgagaac cgaggcgtag aagcgcggcc ccggcgtctc catgccttcg ccgagcttca
      181 tcggcagctt gccgccccac ttggcgtacc agttctgccc gtcgggcagc gagcgggcgt
      241 acgccggggc gtcgagcagc ggctgccatt ccttctggaa gtaagcgtcg gccggcttgc
      301 gggcatggaa gtcgacgacc cactgcgggt gcgccttcat gtagtaggtg gtcgaggcaa
      361 actgcccggt ctgcgcctga tacatgtacg cggtgcccct gtggccggcg ggaaggatcg
      421 cgccgcggtc cttgccggag atcccgatca ccttcgagcg cgcatcgagg cgccgcagca
      481 cgtcccccac ggtttccgcg cgcaggttct tcgggctggt gccgtcgagc cggccggtct
      541 tgtggccgat gtagctcgcg gcgggatcgc cggtgttgta ttcgagctcg ccgctcgccg
      601 ggtcgcgcca ctcgttggcg atgattcccg agcgatgcgg gtacgcgccg gtgagcatcg
      661 tggcgtgccc gggcgcggtc tgggtgttgg agtgcgcgta gtgcgcatcg gcgaaccacg
      721 cgccgcgctc gaggaagcgc cgcaaaccgt cgggggcgag ctgatcgcga tagtcgacca
      781 cctgccgttg cggcaggccg tcgaccacca ggaacaccac gagccggggt ttctccgcgg
      841 cggccggcgc cagcggggcg gcgcatccgg ccagcagcaa tgcggcggca agcgccgcga
      901 caaccaatcg ggaaattgga gccatgagat tcctcccgct cggattttcc actaattttg
      961 ctctagcatc ccccaccgat gatcgacgcg ctggtccagg gcttgctgct ggtgctgcag
     1021 tggaatgcgt tcagcctgat gctgatcggg atggcgatcg gcttcgtggt cggcctgctg
     1081 ccgggcatcg gcggcgccgc gacgctcgcg ctgatgctgc cgttcgtgtt caagatgagc
     1141 ccggtcgagg cgttcgcgtt cctgctgggg atgcactcgg tggccgcgac cacgggcgat
     1201 atcacctcga tcctgttcgg cgttccgggt gagggcctgt cggccgcgac cgtggtcgac
     1261 gggcatccga tggcgaagcg gggcgaagcg gcgcgcgcgc tcggcgcggc cctcatgagc
     1321 tcgcttgtcg gcgcgctgat cggcgcagcg gcgctcgccg tgtcgatccc gatcgtgcgg
     1381 ccgctcgtgc tgaccttcgg ctcgcccgag ctcttcatgg tcacggtgat gggcatcgcc
     1441 tgcatcacct cactgagcgg gcagggcacg cgcgcgcaga tcaagggctt cgcaatgggg
     1501 tttctcggcc tgctgctcgc caccatcggc caggagcgcc aatcgggcat cctgcgcttc
     1561 gacttcggcc tgatctacct ctgggaaggg ctcgacctga tcccggtgct ggtcggcatc
     1621 ttcgccattc ccgagatcat cgacctcgcg gtgcgcggca cctcgatcgc cggcaacgcg
     1681 cccgcggcga agcttggcgc gggggtatgg gaaggcatca aggacacctt caggcatttc
     1741 tggctggtgg tgcgctgctc catggtcggc gtgttcgtcg gcatcctgcc gggcgcgggc
     1801 ggcggggtcg cgcagtggat ggcctatgcc cacgcggcac agagcgcgaa ggacgcgacc
     1861 gaccgcagcc gcttcggcac cggcgacgtg cgcggcgtcc tcgggcccgg cgcggccaac
     1921 aactcgaagg agggcggcga cctcatcccg acgatcgcct tcggcgtgcc gggcagcggc
     1981 gcgatggcga tcctactcgg cgccttcctc atcatggggc tgcatcccgg ccccgacatg
     2041 ctcaccaagc acctcgccgt gacctactcg atggtctgga cgctggtgat cgccaacgtg
     2101 atcaccgtga tcgcatgcct cgcgctcctc ccttatctcg cgtacgtaac gcgcatccgc
     2161 ggcagcgtga tcatcccgtt cctgctgctc ctcgtattcg tcggcagcta taccgcgaac
     2221 aagcagatcg ccgacctgct ggtgacgctc gccgccggcg cgctcggcta cctcatggtg
     2281 cgcttcgaat ggccgcgcgc cccgctggtc ctcggcttcg tcctgggcaa gctggcggag
     2341 acctatcttt tcatctcgat ggcgcgctac ggctactcgt ggctggggca gccgctggtc
     2401 atcgtgctga tggtgctcac ggcggcagtg gtggtgtatc cctatttcca ggaccgcagg
     2461 atccgtgcgg cttagcggcg ccgcggtttt cagcttcggg ctggccgcgg cggccgccta
     2521 cgcggtattc gccgcgctgc gctggccccc caaggctgcg cttttcccgc tcaccatggg
     2581 aatcccgctt ctcgtcctgg ccgtcgcgca aaccgtcgtc gagctgcgag gtccgtcggg
     2641 gcagagcaac cccgacggcg caacgcgcag ggctttcgcg gtatttgcgt ggatggcgag
     2701 cttcatcatc ctggtcctgc tcgcggggtt tcccatcgcg gtgccggttt tcgtattctt
     2761 gtatctcgtc atggaaagcc gggagaagct ggtgctgtcc atcgtcctcg ccgccgcggc
     2821 atggggaggg ttctacctgc tcttccagcg gctgctccat tttccgttcg aggacgggtt
     2881 gatcagaggc tggttccaat aaaaggagga gtgatgaagg cgagactcat cgcggcgatc
     2941 gccgcgctca cggtcactgc gccggcctgg gcgcagggct cgttctacga aggcaagacc
     3001 gtcaccgtgg tgatcggcgc ctcgggcggc agtctcgaga tcgcggcgcg catcgtcgca
     3061 cgccatctcg gcaagcacat cccgggaaac cccagcgtca tcgtccagaa catgaccggc
     3121 gccgcacacc tcgtcgccac caaccacgtc tacaacgtcg ccaagcccga cggcctgacg
     3181 ctgctcgccg ccaacccgaa cgtgggcatc gcgcagctca ccaaggtcga ggcggcgcgt
     3241 ttcgatgcgc ggcgcttcca gtggctcggg tcctccggcg ccgacggcgt cgcgctctcg
     3301 atccgcgccg acctgcccta caagagctgg gacgaggtca agaagtccgg caaggagctc
     3361 gtcgtcggca ccaccgggcc gggatccaac gcgcacgact tcccgctgct cctgaaggag
     3421 ttcgccggcg cgaatttcaa gctggtgtcg ggctatccgg ccaacgccga cgtgctgctc
     3481 gccatcgagc gcaaggaagt cgacgtctgg gccgccttcg ccaccacgat caagctcgcc
     3541 gccgaccgcg gcgcggtgcg gccgctggtg cgcgggcgcg tagcgcttcc cggttgggag
     3601 cacctgccgg tcgacgagga gcttgccacc agcgcggtcg gcaaggcgat catgggcatc
     3661 cgcgccgcgc cgctcgcgat cggccgcgcc gtcgctgcac cgccgggaac gccggtagac
     3721 cgcgtgcagg tcttgcgcga ggccttcgcg aaggcggtca aggatcccga gttcgccgcc
     3781 gagtcgaaga aggcgaagat cgagatcggc tacatcgggc cggaagaggt cctgaagatg
     3841 ttcaacggcc tcctcgacca gcccgccgaa gtgcaaaaag agatggtgaa atacatcaaa
     3901 ttcggcagct aatcgacctc gctcaggaag gtgcggggcg attcggaggc ggtgacg
//
LOCUS       DATBSZ010000295        27650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1085355, whole genome shotgun sequence.
ACCESSION   DATBSZ010000295 DATBSZ010000000
VERSION     DATBSZ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 27650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27650
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085355"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1345)
                     /locus_tag="VIG70_19320"
     CDS             complement(77..1345)
                     /locus_tag="VIG70_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY8252746.1"
                     /translation="MPFRFGVVTLTEAPQAFARVTIVLEDKKSSEGAAAELLAPKWFD
                     KNPALSNEDNFEQLRASMRKARTDYRAGGVNTAYGHSRPAVGLVENFGPALLDRALLD
                     ALCRALGVSFFRAVRANAPGIAFPDLDMPRFLAGLQSRHEIAARHTVGLVDPISSSEQ
                     RVNDGLPETLEEVIARYGHRWFKLKVGGDAKADVERLSAIARVLDRIPGDYHASLDGN
                     EQYEDVQGVLELWRRVREVPSLERLASNVVFIEQPIKRQKALAADISALAAEKPVIID
                     ESDDSLDAFPRAKALGYRGVSSKTCKGIYKSLINAARCAAWGKGYFMSGEDLTIQAGL
                     ALQQDLALVSVLGLTHVERNGHHYVNGMAGLPADEQERFLKSHADLYERSHGAVRVHI
                     RDGKVQLGSLDCPGYASRAMPDWNHMKEMP"
     gene            complement(1399..2175)
                     /locus_tag="VIG70_19325"
     CDS             complement(1399..2175)
                     /locus_tag="VIG70_19325"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8252747.1"
                     /translation="MSEPYIRLRGVGKTYETKDGAVDACAGIDLDVRQSEFVAIVGPS
                     GCGKTTILKMVAGLVPRSAGEITVGGRRVERPQTDVGIVFQESILLDWRDVLSNVMLQ
                     VDIRRMDRAAYEPVARHLLRSTGLEGFEHKKPYELSGGMRQRVSICRALVHDPPLLLM
                     DEPFGALDALTREQISMDIQRVWMEKRKTAMHITHSIPEAVLLADRVVVMGPRPGRIV
                     EILEIDLPRPRRLDRLPPRFNDYAGRIRDIFKAKGVLAMD"
     gene            complement(2186..3199)
                     /locus_tag="VIG70_19330"
     CDS             complement(2186..3199)
                     /locus_tag="VIG70_19330"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8252748.1"
                     /translation="MKSLFRWSLLAALAAGVSTPVGAQQKFQFRLNWTLYGEHAPFFV
                     ARDKGFYREEGLEVEIQEGSGSTTVSQLVANATSPVAYVDAATMMRGVGAGMPIKAVG
                     VTLQQSPMAFIYRADAARPTKISEIKGSRIAITAGDASLAIFTAFMGKLGMKVEDVQM
                     ITVANPAAKEQAVLAKQADALLGYFMDQGPRMQLQTGVKMGWTRLYDMAGVTTLSSAI
                     IANNDWLKVASNQDALRRFLRASQRGWQYSFDNRAEAAEIFRKAAPVFTQEIALLEVD
                     GTMTIIRTERTKGKPIAWSDAGDWKDSQDLLEKFAKLKPQPDVNVYYTNSYLSEAPYM
                     PKK"
     gene            complement(3226..4002)
                     /locus_tag="VIG70_19335"
     CDS             complement(3226..4002)
                     /locus_tag="VIG70_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8252749.1"
                     /translation="MAKLISKLTPLAGILVFLAIWEAAVAVLKVPAYLLPPPTEIVRV
                     FVNDFAKLAFHGWVTAYEMLLGYALAVGIGIPLAIAITSSERFDRFVMPQMLFFQVVP
                     KVAIAPLFLVWFGVGALPKVLVAFLISFFPIVIDAAVGLRSMSAEMRDLARSMGASRL
                     QVFAQFRLPTSLPYLFSGLKVAATLAVAGAVVGEFVGADKGLGYLLLVTNSNLETALM
                     FATLFALTFIGLAFFYAVEFLEALLIPWHVTHRVREEGTM"
     gene            complement(3990..5081)
                     /locus_tag="VIG70_19340"
     CDS             complement(3990..5081)
                     /locus_tag="VIG70_19340"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY8252750.1"
                     /translation="MSRIQRIEVGRLDYSLVGEFKFFKAGKRPSVVVRMTDDVGNCGW
                     GQAVPVETWTYETAETVESTLARYLAPALLGVEPSDLAEVHARMERAIRPSFSVGQPL
                     AKAALDLACYDLWGKQSGLSVSALLGKPSIAEIKLSWTVNAGSLEKAHEQLEQGSAAG
                     YSSFNVKLGPPQTPAHDRDLIRAVRDFAPRGFHWADANTSYDLPTALEMAPRLAHAGF
                     RALESPLPPNRLRDYQALKRLGALPILMDEGIVSPVEAEEFAALGMLDGVAMKVARCG
                     GLWPASRIVAVLKSHGLYVFASGLTDPDLSLAASLHLFACAGLEFPAALNGPQYLDMQ
                     FRPRKDVMQVPTGPGLGFDIDEKTVRWLS"
     gene            5121..6131
                     /locus_tag="VIG70_19345"
     CDS             5121..6131
                     /locus_tag="VIG70_19345"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEY8252751.1"
                     /translation="MAGPRILTTVVGSYPVPDWLVAAPSEQALLDATRVVIHLQEHAG
                     IDLVCDGELYRFDVNHPETNGMIEYFVRPLGGVRAEIGFSEWMEYARGAATRFRTRPP
                     GVVEGPIGAGSLNLPVACARARAMASRPFKFTLTGPHMLAKMLHDLHYRDPEKLAHAI
                     AEVLAEQVARVDADVVQLDEANLPGHPDEWKWAAAAINKVLAAVPAKAKGAVHLCFGN
                     YGGQTIQKGGWDKLLDYLNALRVDHVVMETAHRPPEELAVFKGLRPEIGFGLGVVDIK
                     RTEVESADAIARAIERADKVLGKGRVRYIHPDCGFWMLKRNMADGKTRALAKGRDLYE
                     GR"
     gene            complement(6141..6971)
                     /locus_tag="VIG70_19350"
     CDS             complement(6141..6971)
                     /locus_tag="VIG70_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY8252752.1"
                     /translation="MRIALCNEVIAPMPFPQQCEYAAKLGYDGLEIAPYTLSEEPHGM
                     GAARIAAARAAAEDAGIAVTGLHWLLIKPAGLSISSKDESVRKRTVDVMLALVDLCAE
                     LGGKYLVHGSPQQRRVEAGETRAAAMARARECFAAVAERAQKAGVVYCIEPLSSEQTP
                     LINTLEEASQMVSEIANPAVRSMLDCSSAGRMEKEPLNALVDRWLPNGVIAHVQVNDR
                     NRRGPGQGEQRFAPLFASLKKNGYAGDVAVEPFDYVPDGPGAAARAIGYIKGILEALE
                     "
     gene            complement(6952..8442)
                     /locus_tag="VIG70_19355"
     CDS             complement(6952..8442)
                     /locus_tag="VIG70_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY8252753.1"
                     /translation="MFEGLVLFGKSIGGFLTVESILWALGASLIGVVIGALPGLTATM
                     GVALMTTLTLKMEPNQALLVLVCTYVGAIYGGSRSAILLNIPGTPASAASCLDGYALA
                     RQGRAGYAMGIATTGSVMGTLIGMFFLALFTPLLGNVALKFGAYEFFWLAAFGVVISG
                     NLTGDDPLKGWMAGFLGLFLATIGQEGVYAFERFTFGTRDLAGGLSLVPALVGAFGFA
                     ELLVVMRERRPPVELSPVDSVVPKIRDVLKYWRTIIRSGCIGTFIGIIPGVGEDSAAW
                     TSYAAAKRASAEKELFGKGSVEGLMAAETGDNACVPGAIIPVLTLQIPGSAPAAVLMA
                     AMLIHGVRPGPLIMVESPQFVYDIIAMMLFAVVGILIYGLVLTRFLVQVLRVRYQIIV
                     PVIFVLCAVGSFAIAARLFDVWVMLGFGVLGFVLRLANYPMAPLVLGLVLGDLLEKSL
                     RRGLVLSDGDVTPFFTRPISALLCALIVLIVLSRFIRWRPHANRPV"
     gene            complement(8446..8877)
                     /locus_tag="VIG70_19360"
     CDS             complement(8446..8877)
                     /locus_tag="VIG70_19360"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY8252754.1"
                     /translation="MSDRAQGAAWIAVGGAIVYASWTMDRLERHGAALYTAPGLVPGL
                     LGLVLLVLGVVLAVRNRAAPSLRLRWENTPLVLVLCLGYAIGLVGRVPFWLATFVFVT
                     VFIAAFEYPSRRRMTLAPVYGAATSLAVTYLFETVFLVRLP"
     gene            complement(8874..9875)
                     /locus_tag="VIG70_19365"
     CDS             complement(8874..9875)
                     /locus_tag="VIG70_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8252755.1"
                     /translation="MRIFQALGFAAVLSASTLSQAQNWKPTKPVTIIVPWAAGGSTDQ
                     VTRITAAELEKHLGQKVVIVNQAGASGSIGTKSALEAPKDGYTWTAGAAQDLGVYRAL
                     GMLETNLADWNLFLSVANVPLVGVNPNTPYKSMKDLLDAMKAKPGEITVATAGVTSGG
                     HNAMEAIARATGVKYRHVTYDGGNPAVVATVAGETQVTTQLAVEQAEMIRGKRLRPLA
                     VVADKPLEIEGHGKIDAITQTVPSMKIPTNYFGIFVPKGVPPQVVATLDRIWGDHIMR
                     SEALKKYATSRGALFAPMSGQDAQKAAMPAVQANAWLLHAGGKTKVSPDSVGIPKPD"
     gene            complement(9878..10513)
                     /locus_tag="VIG70_19370"
     CDS             complement(9878..10513)
                     /locus_tag="VIG70_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8252756.1"
                     /translation="MAVLKKANGRDPERTRASILASATAEFARYGLGGARVDRIAARA
                     GANKRMLYYYFGDKEGLFLAALEDRYAHIRNAERSLKLEHLDPQQAVRRLVEFTWRYF
                     LEHPEFMTLLNSENLHQGRHVRRSRRVHEMHSHLVSMLRDVLRRGERQGLFRKGVDPV
                     QLYISIAGEGYFYLSNRYTLSRIFARDLMAPRALSGRARHISDMIVNALRR"
     gene            complement(10501..11511)
                     /locus_tag="VIG70_19375"
     CDS             complement(10501..11511)
                     /locus_tag="VIG70_19375"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY8252757.1"
                     /translation="MNTAEAHALLRRGAVIPAHLLALDARRKLDPRRQRALTRYYRDA
                     GAGGLAVGVHATQFAIREAGLYRPVLELAMEESQGGVAMIAGLAGKTSAAKREAQVAR
                     ELGYHAGMLSLGAMKGASIDALIRHCAAVAEEIPLVGFYLQPAVGGIPLPEPFWRRFA
                     AIENVIAIKIAPFDRYRTLDVVRGVVAARAEERIALYTGNDDHILLDLAVPFSVKRDG
                     EEVRVRIVGGLLGHWGVWTKTAVQLFERVKAGDIDLALDSRITDCNSAFFDVANNFAG
                     CIPGCHEVLRRQGLLEGTWCLDPNERLSPGQAEEIDRVYRDHADLSDDGFVRANLERW
                     LS"
     gene            complement(11537..12349)
                     /locus_tag="VIG70_19380"
     CDS             complement(11537..12349)
                     /locus_tag="VIG70_19380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8252758.1"
                     /translation="MAVTEVRLGADDIDGCVALSAAAGWNQNAADWRLMLELGEGWGL
                     QLRDGTLVASTLVIPYGSFAWVSMVLVLPGHRRKGYASQLLRVALAALARASLTPVLD
                     ATPAGREVYRQEGFRDAWGFKRFVVQRRAQQPESSRVRALREADWPWILELDRAAFGG
                     SRGPLLRSLASRLPVAALVVEGEGFLFGRDGREATQLGPLVATSAATAQSLFKTALAQ
                     AWGPLYLDIADHAVLPPEGLAEQRSFTRMVHGAANVPGERTKVFLVAGPELG"
     gene            complement(12336..13346)
                     /locus_tag="VIG70_19385"
     CDS             complement(12336..13346)
                     /locus_tag="VIG70_19385"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8252759.1"
                     /translation="MFTGIAELEDFMTRPTPELSADLERADGDLIVLGVGGKMGPTLA
                     QMAKRAAPGKRVVGVARFSEKGLRKKLEDQGIECIPCDLLDRAALERLPRPKNVVFMA
                     GHKFGASGEPWLTWAMNVAVPYMVAETWRDSRIVAFSTACVYPYVETTSSGATEATPT
                     TPPPGDYASSCVGREQMFFHGSRRYGTPGRLVRLSYAIDMRYGVLHDVASNVLAGNPI
                     DLAMGYVNVIWQGDANEQALRLLAHCTAPASPINVTGPERVSVRWLAAELGKRLGQEP
                     LFTGKESETAWLIDTTAAVKLFGAPRVALGTMIDWVADWVSRGGQSLGKPTHFDTRDG
                     RY"
     gene            complement(13360..13806)
                     /locus_tag="VIG70_19390"
     CDS             complement(13360..13806)
                     /locus_tag="VIG70_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2846 domain-containing protein"
                     /protein_id="HEY8252760.1"
                     /translation="MSALKTLLALVIIGILAGCAAQGPLHKEVAASIPTVSAGKGRVY
                     FYRPDTMFGGAVTSDITLNGRVVGKSERGSFFFVEENPGDCKASASTEVERQVSFTLS
                     AGETKYIKSTVSMGALVGRVNLELVSPNDAKAEITELHYTGAPLTK"
     gene            complement(13817..14188)
                     /locus_tag="VIG70_19395"
     CDS             complement(13817..14188)
                     /locus_tag="VIG70_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252761.1"
                     /translation="MKIVCVLSLALLVGCASEVHRVPTQFSASTDAAVIVLDRDLTVT
                     PTLGDPATLKAGSAWVRVGQIPEGPVYAIKNDVFMVKGRHAHEARCVVADDGKLIGFF
                     LPVERAFVPVDPQVQLPINRK"
     gene            complement(14241..14669)
                     /locus_tag="VIG70_19400"
     CDS             complement(14241..14669)
                     /locus_tag="VIG70_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8252762.1"
                     /translation="MNLPFTPPVELASRFVDVEHLPWEKTAYAGIEQKTLLFDRNTGL
                     ITALMRMAPGARLPDHEHVKIEQTYVLEGELHCGEGICKKGQFVWRPAGSRHEAWAGP
                     QGGLFLAMFQIPNRFFKPDGRETDFLGNDWNKSWGSVLKK"
     gene            complement(14666..16105)
                     /gene="pyk"
                     /locus_tag="VIG70_19405"
     CDS             complement(14666..16105)
                     /gene="pyk"
                     /locus_tag="VIG70_19405"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006406491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY8252763.1"
                     /translation="MLRSTKIVATLGPSSSEPAVLERMVRAGVDVVRLNFSHGTSDDH
                     LKRAALVKEISSRVGRTVAIMCDLQGPKIRVGKFKEGKVTLEKGARFILDAGIEIGDH
                     DRAGLDYKELPRDVEPGALLLLDDGKIVLEVTSVRGDEVHTLVRHGGVLSNNKGINRQ
                     GGGLTAPALTSKDMEDIKTATKLQADYLAVSFPKSGADMYMARELMRAAGGKAFLIAK
                     IERAEAVGEAALVDIMRGSDGIMVARGDLAVEVGDASVPALQKKMIRLARQENKLTIT
                     ATQMMESMISSPVPTRAEVSDVANAVLDGTDAVMLSAETASGRYPVEVIEAMDRICVE
                     VEQTQPLNLEQDFLNRVFQRVDQSIAMAALFTAYHLKVKCIASLTESGSTALWMSRLN
                     CGVPIYALTSQTQTRYRCALFRDVFPLMVKYVGHDREELLQEAEKALVSNKVVKEGDL
                     IVLTIGEPIGKAGGTNTMKIVKVGEHRKS"
     gene            complement(16122..16922)
                     /locus_tag="VIG70_19410"
     CDS             complement(16122..16922)
                     /locus_tag="VIG70_19410"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY8252764.1"
                     /translation="MNIAAANIVGREVGPDKQRVDARWLMAYSAALGETSFPHPLFPV
                     CYEWPATRLLRERAGLAPLDARLVHAQHDLVIHRAPTTEVLRISGKVVAARQRRPGAM
                     VVFRFVAHDARDQPVTTTDFTVLYRDVKLEDGERAIEEVDDPPPHRKRLPKVGEIEVA
                     ATAAHVYTECARIWNPIHTDIDYARAAGLPGIILHGTATLALSVSRVLASFGIAPGSV
                     RRVRCRFAGMVLMPSVLTVFASTQGTSLAFETRDARGEAVIERGWIIG"
     gene            complement(16919..17326)
                     /locus_tag="VIG70_19415"
     CDS             complement(16919..17326)
                     /locus_tag="VIG70_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY8252765.1"
                     /translation="MVRQFYVDTPGQGLQEITGKVAAAVDGSGVEEGLCTLFLRHTSA
                     SLLIQENADPTAKRDLERWLNRLVPDGDPFYSHDAEGPDDMPSHIKAALTATSLSIPI
                     QQKKLALGTWQGIYLWEHRRRGSRRELVVHIGA"
     gene            complement(17319..18461)
                     /locus_tag="VIG70_19420"
     CDS             complement(17319..18461)
                     /locus_tag="VIG70_19420"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY8252766.1"
                     /translation="MKFKRMSEVDLRGKRVFIRCDLNVPIEDGRITDDTRIRASVPGI
                     QHALDQGAAVMVTSHLGRPTEGELRPEDSLAPVAERLGTLLKTQVPLVRDWVNRVEVQ
                     PGQVVMLENCRVNKGEKKDDEALARKIAALCDVYVNDAFGTAHRAEATTHGVARFAKT
                     ACAGPLMAAELDALGKALANPARPLVAIVGGSKVSTKLTVLGALAKKVDSLIVGGGIA
                     NTFLLAAGKPIGKSLAEPDLAGEAKRIMKKTQVPLPSDVVVESGKTKAVEAVSKDEAI
                     LDIGPETAKAFAAALKKAGTIVWNGPVGVFEKEPFANGTQALARAVAASDAFSIAGGG
                     DTVAAIHKFKVDKRIDYISTAGGAFLEFLEGKKLPAVAALEARADG"
     gene            complement(18462..19499)
                     /gene="gap"
                     /locus_tag="VIG70_19425"
     CDS             complement(18462..19499)
                     /gene="gap"
                     /locus_tag="VIG70_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY8252767.1"
                     /translation="MIKVAINGFGRIGRMVLRAHYEGMREGRKKHDLRFVAVNDLGDA
                     ETNAHLLKYDTAHGKFPARVEVKDKTIVVNGDAIQVVAERDPAKLPWRSLGVDIVFEC
                     TGLFTSKARAGAHIQAGAKKVIISAPGEKDVDRTVVFGVNHKTLKDSDTVISNASCTT
                     NCLAPLVKAIDDRIGVASGLMNTIHSFTNDQVLTDVFHKDLRRARAAGHNMIPTKTGA
                     AAAVGLVLPHLNGKLDGYSIRVPTINVSIVDLTFVAKKPTTVEEVNRAVKQACDSDLK
                     GILEYNTEPLVSSDFNHNPASSIFDSNLTRVSDGTLVKVSSWYDNEWGFSNRMLDVAA
                     VFAEVGAVAAK"
     gene            complement(19496..21520)
                     /gene="tkt"
                     /locus_tag="VIG70_19430"
     CDS             complement(19496..21520)
                     /gene="tkt"
                     /locus_tag="VIG70_19430"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY8252768.1"
                     /translation="MLSPVVAPTTSPVSEAELANAIRALAMDAVEAAKSGHPGMPMGM
                     AEIAVALWKRHLRHNPANPDWPNRDRFVLSNGHGSMLLYSLLHLTGYALPIEELKRFR
                     QLGSKTPGHPERGCAPGVETTTGPLGQGLANAVGMALAERLLATRFNRPGCEIVDHMT
                     YVFLGDGCMMEGVSHEACALAGTLGLGKLIAVYDDNGISIDSDKGEIHQWYTDDVRRR
                     FEAYGWHLVGPVDGHDIEALDKALKKARREKGRPTLIIAKTVIAKGAPRKANTGAAHG
                     APLGAEEIAATRAAIGWPHAPFVVPEGVYRGWDAREAGKKAERKWNRLLKGYKAAYPD
                     DAAEFERRAAGGLPNQFDELISALISDFNQKGETIATRKASQNVLDALVPALPELVGG
                     SADLTGSNLTMAKTSKVAGREGNGNYVFYGVREFGMCAVMNGLALHGGLIPYGGTFLV
                     FSDYARNALRLAALMGVRAIYVFSHDSIGLGEDGPTHQPIEHAASLRLMPNMQVWRPC
                     DTVETAVAWASALRRKTGPTALLLTRQNVPFAKRDSLEGIEKGGYVLADAPQPRAVLI
                     ATGSEVSLALGAQKQLAESGIAVRVVSMPSTTEFDRQSADYRNSVLPKGLPKVAVEAG
                     VSDYWRKYVGTDGAVVGIDRYGESAPAADVFKHFGFTPENVASAVRSVLQ"
     gene            21582..22475
                     /gene="nudC"
                     /locus_tag="VIG70_19435"
     CDS             21582..22475
                     /gene="nudC"
                     /locus_tag="VIG70_19435"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEY8252769.1"
                     /translation="MIELPFRAPLQFEAACFSQSAGEGHWFAFCGDKLLVELGPLDRP
                     SDDPRFRARPAWARLALRKNHDPLYSAALRTLYLGRLGGVDCWAAELAADAPAPAGTA
                     WEGLRPLFSVLDDDHFALAGRALQLLEWDRTSQFCGRCGTPTEAKREERVRTCPACKL
                     SAYPRVAPAVMALVKREKQILLARSPHFPAGMYSALAGFVEPGESLEQCLAREVAEEV
                     GVRISRARYFASQSWPFPHSLMIAFVAEWQSGEIKPQDGEIEAANWFEVLQLPKLPSK
                     ISIARRLIDAVVDDVKGKNGV"
     gene            22601..23857
                     /gene="speE"
                     /locus_tag="VIG70_19440"
     CDS             22601..23857
                     /gene="speE"
                     /locus_tag="VIG70_19440"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEY8252770.1"
                     /translation="MNGLHLIGDLTGCRCDPQLLLDGEGFRAKCIEMVAAAGLTTMDA
                     TFHTFEGGGFTGTVVLAESHLAIHTWPERQGLTLDVYVCNYSADNSAKARKLFDVLVD
                     YFQPTEAARHEVDRGEHLLMEPLNDSTGFYIKASKQLGEWQTRFQKMAIYETPHYGKL
                     FRLDGFNMTSEKEEFVYHENLIHPALTAHAAPKKVLIIGGGDGGSSEEALKHPSVEQV
                     TMVEIDGDVIEVAKTHFHAVHQGVFDNPKLRVLVDDGMKFVRETQEKFDLIALDLNDP
                     MGPAEALYSPEFFQQLRQALAPGGALTLHIGSPVARPERVAQLAQRLNTVFRVVRPYT
                     MYIPLYGSQWAMAVCSDKLDPKSFTADEIDRRIEARKLTDLRYYNGETHEGVFALPNF
                     VRDLVVPARLKQPARGRRLGVVAAAK"
     gene            complement(23869..25026)
                     /locus_tag="VIG70_19445"
     CDS             complement(23869..25026)
                     /locus_tag="VIG70_19445"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY8252771.1"
                     /translation="MNYGGFWIRFLAYLVDGLIVSIAFLAIVALLGVMGLELISNELI
                     LFAMGVLYYTFMQASARQATFGKALVGLKVGGLNGERISLGRSLAREVAKILSVLTFL
                     LGFIIAGFTNRKQALHDFIATTCVVRVEPGHVAAALAVAAVMLLAPFVAVFMFGAGLV
                     TGVFGGIAGTMLSGPQVAVQTPKPAAPVAKPQPGIATASAPSPAPGPSAAPKTVPVAT
                     PTPVATPTPAVVTPAVATPAVVTPVSAPVVVAQAPAAPKEPAKAEPAPPAEAPMAEEQ
                     PPKPVLVPARPMAPRMPRFNDLTTAVLYGDSSAVEELLRYGKWADKPDSTGLTPLMLA
                     VQMGDAVSAELLLKAGANPNRPASGGNTAMKIARERKETALISLLQRYGGR"
     gene            complement(25071..26402)
                     /locus_tag="VIG70_19450"
     CDS             complement(25071..26402)
                     /locus_tag="VIG70_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8252772.1"
                     /translation="MNKRAEVKEVSLEQAAFWMPFTANRQFKKAPRLLARAEGMYYWT
                     PEGRQVLDGTAGLWCVNAGHCRPKIVEAVKRQVAQMDFAPTFQMGHPIAFEFAERLAR
                     IAPEGFSRVFFTNSGSESADTALKIALAYHRARGEAGRYRLIGRERGYHGVNFGGMAV
                     GGITANKKFFGPGVAGVDHIRHTHDPARNAFSRGQPKHGQEFADELERLCLLHDPSTI
                     AAVIVEPVAGSAGVLVPPVGYLERLREICTRHGILLIFDEVITGFGRLGKPFGSQHLG
                     VMPDLFTTAKGMTNATVPMGAVFIKDSIFEAFMQGAEGIELFHGYTYSGHPLACAAGV
                     ATLDTYEEEGLLTRVSTIEKHWENTAHALKGAPHVADIRNCGLMAAIELEPRPGQPGK
                     RAYEVFVKAFEAGVLIRVTGDTIALSPPLIVEKDQIDRIFECIRTMLRSVN"
     gene            complement(26473..27321)
                     /locus_tag="VIG70_19455"
     CDS             complement(26473..27321)
                     /locus_tag="VIG70_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8252773.1"
                     /translation="MKLCRYGKAGYEKPGVIDSEGRLRDLSKVIDNIGPNEISPRGLK
                     MLTRLKPESLPPVNGSPRFGVPYVGIGKFVAIGLNYSDHAKEAGLPIPTEPIVFMKAT
                     TCINGPSDDVIQPKNSTKLDWEIELGVVIGTQAKYVSESDALNHVAGYCIINDVSERA
                     FQMATTQWDKGKGFDTAGPIGPWLVTTDEISDPQTLDMRLDVNGKRMQTGNTRTMVFS
                     VAKIISHVSHYMTLLPGDVIATGTPPGVGLGIKPNPVFLKPGDLMTLGIQGLGEQRQK
                     VVGYSG"
     gene            complement(27318..>27650)
                     /locus_tag="VIG70_19460"
     CDS             complement(27318..>27650)
                     /locus_tag="VIG70_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8252774.1"
                     /translation="SSIRGLPNRFVYGASKAAVIGLTKAIAADYVGKGIRCNAICPGT
                     VDTPSLQGRINAFADPVQARKDFVARQPMGRLGTVDDIMGILVFLASDESLFATGNAY
                     SIDGGMTI"
BASE COUNT         4529 a         9252 c         9231 g         4638 t
ORIGIN      
        1 tcgcggaaga acccgaccgt gcggtcgagg ttcgaaacgg tgaagctcgt gtggttggtg
       61 ccgagtacgc gaaagctcat ggcatttcct tcatgtggtt ccagtcgggc atggcgcggc
      121 tggcgtagcc tgggcagtcg agggatccga gctgcacctt gccgtcgcgg atgtggacgc
      181 gcacggcgcc gtggctgcgc tcgtatagat cggcatgcga cttcagaaac cgctcctgct
      241 cgtccgccgg cagccctgcc atgccgttca cgtagtggtg gccgttgcgc tcgacgtgcg
      301 ttaggccgag gacggagacc agcgcgagat cctgctgcag ggcgaggccc gcctggatgg
      361 tgagatcctc gccgctcatg aagtagccct tgccccaggc ggcgcagcgc gccgcgttga
      421 tgagcgactt gtagattccc ttgcaggtct tggacgacac gccgcgatag ccgagcgctt
      481 tcgcgcgcgg gaacgcgtcg agcgagtcgt cggattcgtc gatgatcacc ggcttctcgg
      541 cagcgagcgc ggagatgtcc gccgcaagcg ccttctgccg cttgatcggc tgctcgatga
      601 acaccacgtt ggaggcgagg cgttccaggc tcggcacttc ccggacccgg cgccagagct
      661 cgagcacgcc ctgcacgtcc tcgtactgct cgttgccgtc gagcgaggca tgatagtcgc
      721 ccggaatgcg atcgagcacg cgcgcgattg cgctcaggcg ctcgacatcg gccttcgcat
      781 cgcccccgac cttgagcttg aaccagcggt ggccgtagcg agcgatgacc tcctccaggg
      841 tttcgggcaa gccgtcgttg actctttgct ccgaggaact gatgggatcg accagaccga
      901 cggtgtgccg ggccgcgatc tcatgacggg actgcaggcc ggccaggaag cgcggcatgt
      961 cgagatcggg aaaagcgatc cccggcgcgt tcgccctcac cgcccggaag aaagaaacgc
     1021 cgagggcgcg gcacagcgca tcgagcagcg cgcggtcgag cagcgccggg ccgaagttct
     1081 ccacaaggcc gaccgcaggc cgggaatgcc cataggcggt attgactcct cccgcccggt
     1141 agtcggttct cgccttgcgc atcgaggcgc gcaattgctc gaagttatcc tcgttgctga
     1201 gcgccgggtt cttgtcgaac cacttcggcg ccagcagctc ggccgcggcc ccttccgagg
     1261 attttttgtc ttcgagaacg atcgttaccc ttgcaaacgc ctgcggcgct tccgtgagcg
     1321 tgaccacgcc gaagcggaat ggcatgcgca ggcgaacctg tctttcaaaa aaatcaatct
     1381 gtttgatcga gaatcgcatc agtccatcgc cagcacgccc ttggccttga agatgtcgcg
     1441 gatccgtccg gcgtagtcgt tgaagcgcgg cggcagccgg tcgaggcggc gcggccgcgg
     1501 caggtcgatc tccagaatct cgacgatgcg gccggggcgc gggcccatca ccaccacgcg
     1561 gtcggcgagc agcaccgcct cgggaatgct gtgcgtgatg tgcatcgcgg tcttgcgctt
     1621 ctccatccat acgcgctgga tgtccatcga gatctgctcg cgggtgagcg cgtcgagcgc
     1681 gccgaagggc tcgtccatga gcagcagcgg cgggtcgtgc accagggcgc ggcagatcga
     1741 cacgcgctgg cgcatgccgc ccgaaagctc gtaaggcttc ttgtgctcga agccctcgag
     1801 gccggtggag cgcagcaggt gccgggccac cggctcgtag gcggcgcgat ccatgcgccg
     1861 gatgtcgacc tgcagcatca cgttcgacag cacgtcgcgc cagtcgagca ggatcgactc
     1921 ctggaacacg atgccgacgt cggtctgcgg ccgctcgacg cgccggccgc cgaccgtgat
     1981 ctcgcccgcg ctgcgcggca ccaggcccgc gaccatcttg aggatcgtgg tcttgccgca
     2041 gccgctcggg ccgacgatgg cgacgaactc cgactgccgg acgtcgaggt cgatgccggc
     2101 gcaggcgtcg accgcgccgt ccttggtctc gtaggtcttg ccgacgcccc gcagccggat
     2161 gtaaggctcg ctcacgtcgg catttctact tcttgggcat atacggcgct tcagacaggt
     2221 agctgttggt gtagtacacg tttacgtcgg gctggggctt gagcttggcg aacttctcca
     2281 gcaggtcctg gctgtctttc cagtcgcccg cgtcggacca ggcgatcggc ttgcccttgg
     2341 tgcgctcggt gcgaatgatg gtcatggtgc cgtcgacctc gagcagcgcg atctcctgcg
     2401 tgaacaccgg cgcggccttg cggaagatct cggcggcctc ggcgcggttg tcgaagctgt
     2461 actgccagcc gcgctgcgag gcgcgcagga agcggcgcag cgcatcctgg ttgcttgcga
     2521 ccttgagcca gtcgttattg gcgatgatcg cgctcgagag cgtggtgacc ccggccatgt
     2581 cgtagaggcg agtccagccc atcttcacgc cggtctgcag ctgcatgcgc ggcccctggt
     2641 ccatgaagta gccgagcagc gcgtcggcct gcttggcgag caccgcctgc tccttggccg
     2701 ccggatttgc gacggtgatc atctgcacgt cctcgacctt catgccgagc ttgcccatga
     2761 aggcggtgaa aatggcgagg ctcgcgtccc ccgcggtgat cgcaatgcgg ctgcccttga
     2821 tctcggagat cttggtcggc cgcgcggcgt cggcgcggta gatgaacgcc atcgggctct
     2881 gctgcagcgt cacgccgacc gccttgatcg gcatgcccgc gcccacgccg cgcatcatgg
     2941 tggcggcgtc gacgtaggcg accgggctgg tcgcgttggc gacgagctgc gacacggtgg
     3001 tcgagccgct gccttcctgg atctcgacct cgagcccttc ctccctataa aagcccttgt
     3061 cgcgcgcgac gaagaacggc gcatgctcgc cgtacagggt ccagttgaga cggaattgaa
     3121 acttctgttg cgcgcctacc ggggtgctca cgcccgcggc cagcgctgcc agcaacgacc
     3181 acctgaacag actcttcatg agttcctcct cctcttgaat agtggctaca tcgttccttc
     3241 ttcgcgcacg cggtgcgtca cgtgccacgg gatcaggagc gcttccagaa attcgaccgc
     3301 gtaaaagaac gccaggccga tgaacgtcag cgcaaacagc gtggcgaaca tcagcgccgt
     3361 ctccagattc gaattggtca ccagcagcag gtagcccagg cccttgtcgg caccgacgaa
     3421 ctcgcccacc acggcgccgg cgacggcgag cgtcgccgcg accttgaggc cgctgaagag
     3481 atacggcagg ctggtcggca gccggaactg tgcgaacacc tgcaggcggc tcgcgcccat
     3541 cgagcgcgcg aggtcgcgca tctcggccga catcgagcgc agccccacgg cggcgtcgat
     3601 cacgatcggg aagaacgaga tcaggaatgc gaccagcacc ttgggcagcg cgccgacgcc
     3661 gaaccagacc aggaacagcg gcgcgatcgc gaccttgggg accacctgga agaacagcat
     3721 ctgcggcatc acgaagcggt cgaagcgctc cgacgaggtg atcgcgatgg cgagcggaat
     3781 tccgatcccg accgcgagtg cgtagccgag cagcatctcg taggcggtga cccagccgtg
     3841 gaacgcgagc ttggcgaaat cgttgacgaa gacgcgcacg atctcggtcg gcggcggcag
     3901 gaggtaagcc ggcaccttga gcaccgcgac cgccgcctcc cagatggcga ggaacaccag
     3961 gattcctgcg agcggggtca gcttcgagat caacttagcc atcgcaccgt cttttcatcg
     4021 atgtcgaagc ccaggcccgg tcccgtcggc acctgcatga cgtctttccg gggacgaaac
     4081 tgcatgtcga gatactgcgg accgttcagc gccgccggga actcgaggcc cgcgcaggcg
     4141 aaaagatgca gcgaagcggc gagcgaaagg tcgggatcgg tcagccccga ggcgaacacg
     4201 taaagaccgt gggatttcag gaccgcgacg atgcgcgagg ccggccagag cccgccgcag
     4261 cgcgcgacct tcatcgccac gccgtcgagc atgccgagcg cggcaaactc ctccgcctcg
     4321 accggcgaaa cgatgccttc gtccatcagg atcggcagcg cgcccaggcg cttcagggcc
     4381 tggtagtcgc gcaggcggtt cggcggcagc ggtgactcga gcgcccggaa gccggcatgc
     4441 gccagcctgg gcgccatctc gagcgcggtc gggaggtcgt agctggtgtt ggcatcggcc
     4501 cagtggaagc ccctaggcgc gaaatcgcgc acggcgcgaa tcaggtcccg gtcatgagcg
     4561 ggggtctggg ggggaccgag cttgacgttg aagctcgagt agcccgcggc gctgccctgc
     4621 tcgagctgct cgtgcgcttt ctcgaggctg ccggcgttga cggtccagct gagtttgatt
     4681 tccgcaatgg agggttttcc aagcagcgcc gacactgaaa gacccgattg cttaccccac
     4741 aagtcgtaac aggctagatc gagcgccgct ttcgccagcg gctggccgac cgagaacgac
     4801 gggcggatgg cgcgctccat gcgcgcatgc acctccgcca ggtccgacgg ctcgacgccg
     4861 agaagcgcgg gcgcgagata gcgcgcgagc gtcgactcga ccgtctcggc ggtctcgtag
     4921 gtccaggtct ccaccggcac cgcctgtccc cagccgcagt tgccgacgtc gtcggtcatg
     4981 cgcacgacga ccgacggacg tttgccggcc ttgaagaact tgaactcgcc gaccagggaa
     5041 tagtcgagtc ggccgacctc gatgcgctgg attctggaca cgcgggtttt aactaatcgg
     5101 ttaacagtat aatgaaaacc atggccggcc cgcgcattct caccaccgtg gtgggctcgt
     5161 acccggtgcc cgactggctg gtcgcagcgc cttccgagca ggcgctgctc gacgcgacgc
     5221 gcgtcgtgat ccacctccag gagcatgccg gcatcgacct ggtgtgcgac ggcgagctct
     5281 accgcttcga cgtcaaccac cccgagacca acggcatgat cgagtacttc gtgcggccgc
     5341 tcggcggggt gcgcgccgag atcggctttt cggagtggat ggagtacgcg cgcggcgcgg
     5401 cgacgcgctt ccgcacacgc ccccccggcg tggtcgaggg cccgatcggc gccggctcgc
     5461 tcaacctgcc ggtggcgtgc gcgcgcgccc gcgcgatggc ctcgcggccg ttcaagttca
     5521 cgctgaccgg tccgcacatg ctcgcgaaga tgctgcacga cctgcactac cgcgacccgg
     5581 agaaactggc gcacgccatc gccgaggtgc tcgccgagca ggtggcgcgc gtggacgccg
     5641 acgtggtcca gctcgacgaa gcgaaccttc ccggccatcc cgacgagtgg aagtgggcgg
     5701 cggcggcgat caacaaggtg ctcgcggcgg taccggccaa ggcgaagggc gcggtgcacc
     5761 tgtgcttcgg gaactacggc gggcagacga tccagaaggg cggctgggac aagctgctcg
     5821 actacctgaa cgcgctgcgc gtcgatcatg tcgtcatgga aaccgcgcac cggccgcccg
     5881 aggagctcgc ggtgttcaag gggctgcggc cggagatcgg cttcggtctg ggcgtggtcg
     5941 acatcaagcg caccgaggtg gaatcggccg acgccatcgc gcgcgccatc gagcgcgcgg
     6001 ataaggtgct cggcaagggc cgcgtccggt acatccaccc cgactgcggg ttctggatgc
     6061 tcaagcgcaa catggccgac ggcaagacgc gggcgctcgc caaggggcgc gatctctacg
     6121 agggtcgtta acaaagacgc tcactcgagg gcctctagga ttcccttgat gtagccgatg
     6181 gcgcgcgcgg cggcgcccgg cccgtctgga acgtagtcga acggctcgac cgccacgtcc
     6241 cccgcatagc cgtttttctt gagagaagca aagagcggcg cgaagcgctg ctccccctgg
     6301 ccgggcccgc gacggttgcg atcgttcacc tgcacgtggg cgatcacgcc attcggcagc
     6361 cagcggtcga ccagcgcgtt gagcggctcc ttttccatgc gcccggcgga gctgcaatcg
     6421 agcatgcttc tgaccgccgg gttggcgatt tccgagacca tctgggaagc ttcctcgagc
     6481 gtgttgataa gcggcgtttg ctcggaagag agcggctcta tgcagtagac gacgccggcc
     6541 ttctgcgcgc gctcggcgac cgcggcgaag cattctcttg cgcgcgccat cgccgcggcg
     6601 cgcgtctcgc ccgcctcgac gcggcgctgc tgcggcgagc cgtgcaccag gtatttgccg
     6661 ccgagctcgg cgcacaagtc caccagggcg agcatcacat cgacggtgcg ctttctgacg
     6721 ctttcatcct tgctgctgat cgatagaccg gcgggcttga tgagaagcca gtggagtccc
     6781 gtcaccgcga tgccggcgtc ctcggccgcg gcgcgggcag cggcaatgcg cgcggcgccc
     6841 atgccgtgcg gctcctccga aagcgtgtac ggcgcgatct ccaggccgtc gtagccgagc
     6901 tttgccgcgt actcgcattg ctgcgggaag ggcatcggcg cgatgacctc gttacacagg
     6961 gcgattcgca tgaggtctcc aacggatgaa gcgcgacagc acgatcagca cgatcagagc
     7021 gcacaggagg gcggagatcg ggcgggtgaa gaacggcgtc acgtcgccgt ccgagagcac
     7081 cagcccgcgg cgcagcgact tctccaacag gtcgccgagg accagcccga gcacgagcgg
     7141 cgccatcggg tagttggcga gccgcaggac gaagccgagg acgccgaagc cgagcatcac
     7201 ccagacgtcg aacagacgcg ctgcgatcgc gaacgagccg accgcgcaca gcacgaagat
     7261 taccggcacg atgatctggt agcgcacgcg caggacctgc accaggaagc gcgtgagcac
     7321 caggccgtag atcaggatgc cgacgaccgc gaacagcatc atggcgatga tgtcgtaaac
     7381 gaactgcggc gactccacca tgatgagcgg ccccgggcgc acgccgtgga tcagcatcgc
     7441 cgccatcagc accgcggccg gcgccgagcc cgggatctgc agcgtcagca ccgggatgat
     7501 cgctcccggc acgcaagcgt tgtcgccagt ctcggcggcc atcaagcctt ccaccgagcc
     7561 cttgccgaaa agctccttct cggcgctcgc gcgctttgcc gccgcgtagg aagtccaggc
     7621 ggcgctgtcc tcgcccacgc ccggaatgat gccgatgaag gtgccgatgc agcccgagcg
     7681 aatgatggtg cgccagtact tcaagacatc ccggattttc ggcaccacgc tgtccacggg
     7741 gctgagctcc accggcgggc gccgctcgcg catcacgacc agaagctcgg cgaagccgaa
     7801 cgcgcccacc agcgccggca cgagcgacag cccgccggcg agatcgcgcg tgccgaaggt
     7861 gaaacgctcg aaggcgtaca cgccctcctg cccgatagtg gcgaggaaca ggcccaggaa
     7921 tcccgccatc cagcccttca gcggatcgtc gccggtcagg ttgcccgaga tcacgacgcc
     7981 gaacgcggca agccagaaga attcgtacgc gccgaacttg agggccacgt tgccgagcag
     8041 cggcgtgaag agagcaagga agaacatgcc gatcagcgtc cccatcacgc tgcccgtggt
     8101 ggcgatgccc atcgcgtagc cggcgcggcc ctggcgcgcc agcgcatagc cgtccaggca
     8161 cgaggcggcc gacgcgggcg tgcccggaat gttgagcagg atggcgctgc gcgagccgcc
     8221 gtagatcgcg ccgacgtaag tacacaccag caccagcagc gcctggttgg gctccatctt
     8281 cagcgtgagc gtggtcatga gcgccacgcc catcgtcgcg gtcaggcctg gcagcgcgcc
     8341 aatcaccacg ccgatgaggc tcgcccccag cgcccacaga atcgactcga cggtgaggaa
     8401 gccgccgatc gacttcccga aaagcaccag gccttcgaac atgggctacg gcagccgcac
     8461 caggaatacc gtttcgaaca ggtacgtgac ggcgaggctg gtcgcggcgc cgtagaccgg
     8521 cgccagcgtc atccggcggc gagacgggta ctcgaacgcg gcgatgaaga cggtgacgaa
     8581 aacgaacgtc gcgagccaga acggcacgcg gccgacgagg ccgatagcgt agcccaggca
     8641 cagcactagc accaagggcg tgttctccca ccgaaggcgc aaggaaggcg cggccctgtt
     8701 gcgcacggca agcaccacgc cgagcacgag cagcaccagc ccgagcagcc cgggcacgag
     8761 acccggcgcg gtatagaggg ccgcgccgtg gcgctcgagc cggtccatcg tccaggaggc
     8821 gtagacgatc gccccgccga cggcgatcca ggccgcgcct tgggctctgt cgctcaatca
     8881 ggcttgggaa tacccacgga gtccggagag accttggtct tgccgccggc gtgcaggagc
     8941 cacgcgttcg cctgcaccgc cggcatcgcc gccttctgcg cgtcctgtcc cgacatcggc
     9001 gcgaacagcg ccccgcggct ggtggcgtac ttcttcagcg cctccgagcg catgatgtgg
     9061 tcgccccaga tgcgatcgag cgtggcgacg acctgcggcg gcacgccttt cggaacgaag
     9121 atgccgaaat agttcgtggg gattttcatg ctcggcacgg tctgcgtgat ggcgtcgatc
     9181 ttgccgtgac cctcgatctc gagcggcttg tcggccacca ccgcgagcgg gcgcaggcgc
     9241 ttgccgcgga tcatctcggc ctgctcgacc gcgagctgcg tcgtcacctg ggtctcgccc
     9301 gcgaccgtcg ccactaccgc ggggttgccg ccgtcgtagg tgacgtggcg gtacttcacg
     9361 ccggtggcgc gcgcgatcgc ttccatcgcg ttgtggccgc cggaagtcac gcccgccgtg
     9421 gcgaccgtga tctcgcccgg cttcgccttc atcgcgtcga gcaggtcctt catgctcttg
     9481 tagggcgtgt tcgggttcac gccgacgagc ggcacgttgg cgacggagag aaacaggttc
     9541 cagtcggcga ggttggtttc cagcatgccg agcgcgcggt acacgcccag gtcctgcgct
     9601 gcacccgcgg tccaggtata gccgtccttc ggcgcctcga gcgcgctctt ggtgccgatc
     9661 gagcccgatg cgccggcctg gttcacgatc accaccttct gcccgaggtg cttctcgagc
     9721 tcggcggcgg taatgcgcgt cacctggtcg gtggagccgc ccgcagccca cggcacgatg
     9781 atcgtgaccg gcttggtcgg cttccagttc tgggcttggg acaacgtgct cgccgacaag
     9841 acggcggcaa atcccagtgc ttgaaaaatc ctcatgctta cctcctcagt gcgttgacaa
     9901 tcatgtccga aatgtgccgc gcgcgtccgg acagcgcgcg cggcgccatc aggtcgcggg
     9961 cgaagatgcg cgagagcgtg tagcgattgc tcaggtagaa atagccttcg ccggcgatcg
    10021 agatatagag ctgcaccggg tcgacgccct tgcggaacag accctggcgc tcgccccggc
    10081 gcagcacgtc gcgcagcatc gacacgaggt gcgagtgcat ctcgtgcacg cggcgcgagc
    10141 ggcgcacgtg gcgcccctgg tgcaggtttt cactgttgag gagcgtcatg aactccgggt
    10201 gctcgagaaa atagcgccag gtgaactcga ccaggcgccg caccgcctgt tgcgggtcga
    10261 ggtgctccag cttgagcgag cgctcggcgt tgcggatgtg cgcgtagcga tcctccagcg
    10321 cggcgaggaa caggccctcc ttgtcgccga agtagtagta gagcatgcgc ttgttcgcgc
    10381 ccgcgcgcgc ggcgatgcgg tcgacgcgcg cgccgccgag gccgtagcgc gcgaattccg
    10441 cggtggcgga ggcgaggatg ctcgcgcgcg tgcgctcggg gtcgcggccg ttcgcttttt
    10501 tcaggacagc caccgctcga gattggcccg cacgaagccg tcgtcggaaa gatcggcatg
    10561 gtcgcggtac acgcggtcga tctcctcggc ctgccccggc gagagccgct cgttcgggtc
    10621 gaggcaccag gtgccctcga gcagcccctg gcgccgcagc acctcgtggc agcccgggat
    10681 gcagccggcg aaattgttgg cgacgtcgaa gaacgcgctg ttgcagtcgg taatgcgtga
    10741 atcgagcgcc agatcgatgt cgcctgcttt taccctctca aaaagttgaa ccgccgtctt
    10801 ggtccacacg ccccagtgcc ccaggagccc gccgacgatg cgcacgcgca cctcctcccc
    10861 atctcgcttg acggagaaag gcaccgccaa gtcgagcagg atgtgatcat cgttgccggt
    10921 gtagagcgcg atgcgttctt ctgcccgcgc agccaccaca ccccgcacca cgtcgagcgt
    10981 ccggtagcgg tcgaacggcg cgatcttgat cgcgatcacg ttctcgatcg cggcgaagcg
    11041 acgccagaac ggctcgggga gcgggattcc gccgaccgcc ggctgcagat agaagccgac
    11101 cagcgggatc tcctcggcca ccgcggcgca atggcggatc aaggcgtcga tggatgcgcc
    11161 tttcatcgcg ccgagcgaca gcatcccggc gtggtaaccg agctcgcgcg cgacctgggc
    11221 ctctcgcttc gccgcgctgg tttttcccgc gaggccggcg atcatcgcca ccccgccttg
    11281 cgattcctcc attgcgagct ccagcaccgg ccggtagagg ccggcttccc gtatcgcgaa
    11341 ctgggtcgcg tggaccccta ccgccaaacc gcccgcgccg gcatcgcggt aatagcgggt
    11401 gagcgcgcgc tggcggcgcg ggtcgagctt gcggcgcgcg tccagggcga gcaaatgcgc
    11461 gggaatgaca gccccgcggc gcagcaacgc atgcgcttcg gctgtgttca tcctgggttt
    11521 taaccggctg gttactttag ccgagttccg gaccggcaac cagaaatacc ttggtgcgct
    11581 cgccgggaac gttcgccgcg ccgtgcacca tccgcgtgaa cgagcgctgc tcggcgagcc
    11641 cttcgggtgg aagcacggca tggtcggcga tgtcgaggta gaggggcccc catgcctgcg
    11701 ccagcgcggt cttgaacagg ctctgcgccg tggccgcgct cgtcgcgacc agcgggccga
    11761 gctgcgtcgc ctcccggccg tcgcggccga aaaggaatcc ttcgccttcc accaccagcg
    11821 ccgccaccgg caggcggctc gccagcgagc gcagcagcgg cccgcggctt ccgccgaagg
    11881 cggcgcgatc gagctcgagg atccagggcc aatccgcttc gcgcaacgcg cgcacgcgcg
    11941 aggactccgg ttgctgagcc ctcctctgca ccacgaaccg cttgaagccc caggcatcgc
    12001 gaaatccttc ctggcggtac acctcgcggc cggcgggagt ggcatcgagg accggcgtga
    12061 ggctcgcccg cgcgagcgcg gccagcgcga cccgcaggag ctgcgacgca tagcctttcc
    12121 ggcgatggcc cggcagcacg aggaccatgc tgacccaggc aaagctgccg tacgggatca
    12181 ccagggtaga ggcgacgaga gtcccgtcgc gaagctgaag gccccagccc tcgcccagct
    12241 cgagcatcag gcgccagtcg gccgcgttct ggttccagcc ggccgcggcc gagagcgcga
    12301 cgcagccgtc gatgtcgtcg gcgccgaggc ggacctcagt aacggccatc gcgcgtgtcg
    12361 aagtgggtcg gcttgccgag gctttgcccg ccgcgcgaca cccagtcagc gacccagtcg
    12421 atcatcgtgc cgagcgcgac gcgcggcgcg ccgaagagct tgaccgccgc agttgtgtcg
    12481 atcagccacg ccgtttcact ctccttgccc gtaaacaagg gctcctggcc gagccgcttg
    12541 ccgagctctg ccgccagcca gcgcaccgac accctctcgg gcccggtcac gttgatcggg
    12601 gaggccggcg cggtgcagtg cgcgaggagc cgcagcgcct gctcgttggc gtcgccctgc
    12661 cagatgacgt tcacgtaccc catggccaga tcgataggat tgcccgccag cacattcgag
    12721 gcaacgtcat gcagcacgcc gtagcgcatg tcgatcgcgt aggaaaggcg caccaagcgg
    12781 ccgggcgtgc cgtagcggcg cgagccgtgg aagaacatct gctcgcgccc gacgcaggag
    12841 ctcgcatagt cgccgggcgg aggtgtagtt ggggtcgctt ccgtggctcc cgagcttgtt
    12901 gtctccacat atggatacac gcaagcggtg gagaacgcga cgatgcgcga gtctctccac
    12961 gtctcggcca ccatgtaggg cacggcgacg ttcatcgccc aggtgagcca gggctcgccg
    13021 ctcgcgccga acttgtggcc ggccatgaac accacgttct tcggccgcgg caggcgctcg
    13081 agcgccgcgc gatcgagcag atcgcaggga atgcattcga ttccctggtc ctcgagcttt
    13141 ttcctgagtc ctttttccga aaagcgggca accccgacga cgcgtttccc cggcgcggcg
    13201 cgcttcgcca tctgcgcgag ggtcggcccc atcttgccgc cgacgccgag gacgatcagg
    13261 tcgccgtcgg cgcgctccag gtcggccgac agttccggcg tcggccgcgt catgaagtcc
    13321 tcgagttcgg cgatgccggt gaacacgcgt ccgccccggc tacttcgtca ggggcgcccc
    13381 cgtgtagtgg agctcggtga tttcggcctt ggcgtcattg gggcttacca gctcgagatt
    13441 gacccggccg acgagcgctc ccatggagac ggtggacttg atgtacttcg tctcgcccgc
    13501 ggacagcgtg aagctcacct gccgctcgac ctccgtcgac gccgacgcct tgcaatcccc
    13561 aggattctct tccacgaaga agaagctgcc ccgctcggac ttgccgacga cccgcccatt
    13621 gagcgtgatg tccgaagtaa cggcgccgcc gaacatggtg tctggccggt agaaatagac
    13681 tctcccctta cccgcggaaa cggtcgggat cgaagcggcg acctccttgt ggagcggccc
    13741 ttgggcggcg cagccggcga ggattccaat gattacgaga gcgaggagag tcttcaaagc
    13801 ggacatgcat gtctccttat tttcggttta tgggtaactg cacctgtgga tccaccggca
    13861 caaaggctcg ttccaccggc agaaagaacc cgatcagctt gccgtcgtcc gcgacgacac
    13921 atcgcgcctc gtgagcgtgt ctccctttga ccatgaacac gtcgttcttg attgcataca
    13981 cggggccctc cggaatctgg ccgacgcgca cccacgccga gccggccttt agggttgctg
    14041 ggtcgcccaa ggtcggcgtg acggtaaggt cccggtcgag cacgatcact gcagcgtccg
    14101 tggatgcgct gaactgggtc ggcacccgat ggacttccga ggcgcagccg actagcagcg
    14161 ccaatgacag cacgcaaacg atcttcatgc tcccccttga agcgacacct ccgaggcgaa
    14221 tcgggctctg accccgattt ctacttcttc aacacggagc cccagctctt gttccagtcg
    14281 ttgccgagaa aatcggtctc gcggccgtcg ggcttgaaga acctgttcgg gatctggaac
    14341 atggcgagga acaggccccc ttgcggcccg gcccaggcct cgtggcggct gcccgccggg
    14401 cgccagacga actggccctt tttgcagatt ccttcgccac agtgcaactc gccttccaat
    14461 acatacgtct gctcgatctt cacgtgctcg tggtcaggca gccgtgcgcc gggcgccatg
    14521 cgcatcagcg cggtgatgag gccggtattg cggtcgaaga gcagcgtctt ctgctcgatg
    14581 cccgcgtagg cggttttctc ccacggcagg tgctcgacgt cgacaaacct ggatgcgagt
    14641 tcaacagggg gtgtaaaagg caggttcatg atttgcggtg ctccccgacc ttgacgatct
    14701 tcatggtgtt ggtgccgccc gccttgccga tcggctcgcc gatggtgagg acgatgaggt
    14761 cgccttcctt caccaccttg ttcgaaacca gcgccttttc ggcctcctgc agcagctctt
    14821 cgcggtcgtg gcccacatat ttgaccatga gcgggaacac gtcgcggaac agcgcgcagc
    14881 ggtagcgggt ctgggtctgc gaggtgaggg cgtagatcgg cacgccgcag ttgaggcggc
    14941 tcatccacag cgcggtcgag cccgactcgg taagcgaggc gatgcacttc accttgaggt
    15001 ggtacgcggt gaacagcgcc gccatggcga tcgactggtc gacgcgctgg aacacgcggt
    15061 tcaggaagtc ctgctccagg ttgagcggct gggtctgctc gacctcgacg cagatccggt
    15121 ccatcgcctc gatcacctcc accgggtagc gcccgctcgc ggtctcggcc gaaagcatca
    15181 cggcgtcggt gccatcgagc acggcgttcg ccacgtccga gacctcggcg cgcgtcggca
    15241 ccgggctcga gatcatcgat tccatcatct gcgtcgcggt gatagtgagc ttgttctcct
    15301 gccgcgcgag gcggatcatc tttttctgca gcgcgggcac cgaggcgtcg ccgacctcga
    15361 ccgcgaggtc gccgcgcgcc accatgatgc cgtcggagcc gcgcatgatg tcgacgagcg
    15421 cggcctcgcc gaccgcttcg gcgcgctcga tcttcgcgat caggaacgcc ttgccgcccg
    15481 ccgcgcgcat cagctctctc gccatgtaca tgtcggcgcc gctcttcggg aacgagaccg
    15541 cgaggtagtc ggcctgcaac ttggtcgcgg tcttgatgtc ctccatgtcc ttggaggtga
    15601 gcgccggcgc ggtgaggccg ccaccctggc ggttgatgcc cttgttgttc gacagcaccc
    15661 cgccatggcg caccagcgtg tgcacctcgt cgccgcgcac gctggtgacc tcgagcacga
    15721 tcttgccgtc gtcgagcagc agcagcgcgc cgggctcgac gtcgcgcggc agctccttgt
    15781 agtcgaggcc ggcgcggtcg tgatcgccga tttcgatccc ggcatcgagg atgaagcggg
    15841 cgcccttctc gagggtgacc ttgccttcct tgaacttgcc gacgcggatc ttcggcccct
    15901 gcaggtcgca catgatcgcg accgtgcggc ccaccctgct cgagatctcc ttcaccagcg
    15961 cggcgcgctt gaggtgatcg tcggaggtgc cgtgcgagaa gttgagccgc accacgtcga
    16021 cgccggcgcg caccatgcgc tcgagcacgg ccggctcgct cgacgagggc ccgagggtgg
    16081 cgacgatttt tgtactgcgc agcatgaggg gcgctgcgag tttaaccgat gatccagccg
    16141 cgctcgatga ccgcctcccc gcgtgcgtcg cgcgtctcga acgcaaggga cgtgccttgc
    16201 gtggaagcga acacggtcag aaccgagggc atcagcacca tgccggcgaa gcggcagcgc
    16261 acgcggcgca cggagcccgg cgcgattccg aacgaagcga gaaccctcga cacggaaagc
    16321 gcgagcgtcg cggtgccgtg gaggatgatg ccgggcagcc cggcggcgcg cgcgtagtcg
    16381 atgtcggtat ggatcgggtt ccagatgcgc gcgcattcgg tgtagacgtg agccgccgtg
    16441 gcggcgacct cgatctcgcc aaccttcggc aatcgcttgc ggtgcggcgg agggtcgtcg
    16501 acctcttcga tcgcacgctc gccgtcctcg agcttcacgt cccggtagag caccgtgaag
    16561 tccgtggtgg tcaccggctg gtcgcgcgcg tcgtgcgcca cgaagcggaa caccaccatc
    16621 gcgcccgggc ggcgctgcct cgctgccacg acctttcccg aaatgcgcag gacctcggtg
    16681 gtcggcgccc ggtggatgac caggtcgtgc tgtgcgtgca ccaggcgcgc gtcgagcggc
    16741 gccagccccg cgcgctcgcg cagcaggcgg gtagccggcc attcgtagca caccggaaaa
    16801 agcggatgcg gaaagctggt ttcgccgagc gcggcgctgt aggccatgag ccagcgcgcg
    16861 tcgacgcgct gcttgtccgg ccccacctcc cgcccgacga tgttcgccgc cgcgatgttc
    16921 acgcgccgat gtgcacgacg agctcgcggc gcgagccgcg gcggcggtgc tcccagaggt
    16981 agatgccttg ccaggtgccc agcgcgagct tcttctgctg aatgggaatc gagagcgaag
    17041 tggccgtgag cgcggccttg atgtgcgacg gcatgtcgtc gggcccctcc gcgtcgtggc
    17101 tgtagaacgg atcgccgtcc ggcacgagac ggttcagcca gcgctcgaga tcgcgcttcg
    17161 cggtgggatc ggcgttctcc tggatcagga ggctcgcgga ggtatggcgc agaaagagcg
    17221 tgcacaggcc ttcctccaca cctgaaccgt cgaccgcggc cgcgactttg ccggtgattt
    17281 cctgcagccc ctgccccggc gtgtcgacgt agaactgcct aaccatccgc cctggcttcg
    17341 agcgcggcaa ccgccgggag cttcttccct tcgaggaact cgaggaacgc gccgcccgcg
    17401 gtggagatgt agtcgatgcg cttgtcgacc ttgaacttgt ggatcgccgc cacggtatcg
    17461 ccgccgcccg cgatcgagaa tgcgtcggac gcggccaccg ccctggcgag cgcctgggtg
    17521 ccgttggcga acggctcctt ctcgaacacc ccgaccgggc cgttccacac gatcgtgccg
    17581 gccttcttga gcgcggcggc gaacgctttc gcggtctcgg gtccgatgtc caggatcgcc
    17641 tcgtccttcg agaccgcctc gactgccttg gtcttgccgc tttccaccac cacgtcgctc
    17701 ggcagcggca cctgcgtttt cttcatgatg cgcttcgcct cgccggcgag gtcgggctcg
    17761 gcgagcgact ttccgatcgg cttgccggcg gcgagcagga aggtgttcgc gatgccgccg
    17821 cccacgatca ggctatccac cttcttcgcc agcgcgccga gcaccgtgag cttggtcgac
    17881 accttggagc cgccgacgat cgcgacgagc ggccgcgccg ggttggcgag cgccttgccc
    17941 agcgcatcga gctcggcggc catcaggggg cccgcgcagg cggttttcgc aaaccgggcg
    18001 acgccatggg tagtggcttc cgcgcggtgg gcggtgccga aggcatcgtt cacatagaca
    18061 tcgcacagcg ccgcgatctt gcgggcgagc gcctcgtcgt ccttcttctc gcccttgttg
    18121 acgcggcagt tctccagcat cacgacctgg ccgggctgga cctcgacgcg gttcacccaa
    18181 tcgcgaacca gcgggacctg ggttttcagc agagtgccca gcctctcggc cacgggagcg
    18241 agcgagtcct cgggcctgag ctcgccctcg gtggggcggc cgaggtgcga agtgaccatc
    18301 accgcggcgc cctggtcgag cgcatgctgg atgccgggga cggaggcgcg gatgcgcgtg
    18361 tcgtccgtga tgcgcccatc ctcgatcggg acgttgaggt cacagcggat gaaaacgcgc
    18421 ttccctcgga ggtccacctc cgacatccgc ttgaacttca tctattttgc tgcgaccgct
    18481 ccgacttcgg caaaaacggc ggcgacgtcc agcatccggt tggagaagcc ccactcgttg
    18541 tcgtaccacg aggacacctt gacgagcgtg ccgtcggaga cgcgggtgag gttcgagtcg
    18601 aagatcgagc tagccgggtt atggttgaag tccgaggaca ccagcggctc ggtgttgtac
    18661 tcgaggatgc ccttcaggtc cgagtcgcag gcctgcttca ccgcgcggtt tacctcctcc
    18721 acggtggtcg gcttcttcgc cacgaaggtg aggtcgacga tcgagacgtt gatggtcggc
    18781 acgcggatcg agtagccgtc gagcttgccg ttcaggtgcg gcagcaccag gcccacggcg
    18841 gcggcggcgc cggtcttcgt cgggatcatg ttgtgccccg cggcgcgcgc gcggcgcagg
    18901 tccttgtgga acacgtcggt gagcacctgg tcgttggtga acgaatggat cgtgttcatc
    18961 agccccgagg cgacgccgat gcgatcgtcg atcgccttca cgagcggcgc gaggcagttg
    19021 gtggtgcacg acgcattcga gatcaccgtg tccgaatcct tcaaggtctt gtgattcacg
    19081 ccgaacacca cggtgcggtc gacgtccttc tcgcccgggg cggagatgat gactttcttc
    19141 gcgcccgcct gaatgtgggc gccggccctt gccttgctgg tgaaaagccc ggtgcactcg
    19201 aagacgatgt ccacgccgag cgaacgccac ggcagcttgg ccggatcgcg ctccgccacc
    19261 acctggatcg catcgccgtt cacgacgatg gtcttgtcct tgacctccac gcgcgccgga
    19321 aacttcccgt gcgcggtgtc gtacttcagc aggtgcgcgt tggtctcggc atcgccgagg
    19381 tcgttcaccg cgacgaaacg aaggtcgtgc ttctttctgc cttcacgcat tccctcatag
    19441 tgggcgcgca gcaccatgcg cccgattcgt ccgaatccgt tgatggctac cttgatcatt
    19501 gaagaacgct cctcaccgcg cttgcgacat tttccggggt aaagccgaag tgcttgaaca
    19561 cgtcggcggc cggcgccgac tcgccgtagc ggtcgatgcc gaccaccgcg ccgtcggttc
    19621 cgacgtactt gcgccaataa tcgctgacgc ccgcttccac cgctaccttt gggagtccct
    19681 tcggcagcac agagttccgg taatcggcgc tctggcggtc gaattccgtg gtcgagggca
    19741 tcgacaccac ccgcaccgcg atccctgact cggcgagctg cttctgcgcg cccagggcga
    19801 gggacacctc cgagccggtc gcgatcagca ccgccctcgg ctgcggggcg tcggcgagca
    19861 cgtagccgcc cttttcgatg ccctcgaggg aatctcgctt cgcgaatggg acgttctggc
    19921 gggtcagcag gagcgccgtg gggccggtct tgcgccgcag cgccgaggcc caggcgaccg
    19981 cggtctcgac cgtgtcgcag ggccgccaga cctgcatgtt cggcatgagg cgaaggctcg
    20041 cggcgtgctc gatcggctgg tgcgtcggcc cgtcttcgcc caggccgatc gagtcgtgcg
    20101 agaacacgta gatcgcccgc acgcccatca acgcggcgag gcgcagggcg ttacgcgcgt
    20161 agtcggagaa cacgaggaac gtgccgccgt aggggatgag gccgccgtgc agcgcgaggc
    20221 cgttcatcac cgcgcacatg ccgaattccc gcacgccgta gaacacgtag ttgccattgc
    20281 cctctcgccc ggcgactttc gaggtcttgg ccatggtgag gttcgagccg gtgaggtcgg
    20341 ccgagccgcc gacgagctcc ggcagcgccg gaaccagcgc atccagcacg ttctgcgacg
    20401 ccttgcgcgt ggcaatggtc tcgcccttct ggttgaagtc ggaaatcagg gccgagatca
    20461 gttcgtcaaa ctggtttggc agtccacccg cagcacggcg ctcgaattcg gcggcgtcgt
    20521 cgggataggc tgccttgtag cccttgagga gcctgttcca cttgcgctcg gccttcttgc
    20581 cggcttcgcg cgcgtcccaa ccgcggtaga ccccctccgg gaccacgaac ggcgcatgcg
    20641 gccagccgat ggcggcgcgg gtcgcggcga tctcttcggc accgagcggc gcgccgtgcg
    20701 ccgcgccggt gttcgccttc ctgggcgcgc ccttggcgat gaccgtcttg gcgatgatca
    20761 aagtcgggcg tcccttctca cgccttgcct tcttgagcgc cttgtcgagc gcctcgatgt
    20821 cgtggccgtc gaccggcccg accaggtgcc agccgtaggc ctcgaagcgc cggcgcacgt
    20881 cgtcggtgta ccactggtgg atctcgccct tgtccgagtc gatggaaatg ccgttgtcgt
    20941 catacacggc gatcagcttg cccaggccga gcgtcccggc gagcgcgcag gcctcgtgcg
    21001 agacgccttc catcatgcag ccgtcgccga ggaacacata cgtcatatgg tcgactatct
    21061 cgcatccggg gcggttgaag cgcgtggcaa ggagccgctc ggcgagcgcc atgcccaccg
    21121 cgttcgcgag tccctgcccg agcgggccgg tggtggtctc gacgcccggc gcgcagccgc
    21181 gctcggggtg gcccggggtc ttcgagccga gctggcggaa gcgcttcagc tcctcgatcg
    21241 gcagcgcgta gccggtcagg tgcagcaggc tgtagagcag catcgagccg tgcccattgg
    21301 agagcacgaa gcggtcgcgg ttgggccaat cggggttcgc cgggttgtgg cgcaggtggc
    21361 gcttccacaa ggccacggcg atctccgcca tgcccatcgg catgcccggg tggccggact
    21421 tcgccgcctc caccgcatcc atggcgagcg cgcgaatggc gttggctagc tcggcctcgg
    21481 aaacggggga agtggtcggg gccactacgg gagaaagcat ggagacatgc aaattatact
    21541 cagcgacatc aggcgaaatg agggcgaaat gagatgaggc aatgatcgag cttcccttcc
    21601 gcgcgccgct gcagttcgag gccgcttgct tttcccaaag cgcgggcgag ggccactggt
    21661 tcgccttctg cggcgacaag ctgctggtcg agctcggtcc gctcgaccgg ccgagcgacg
    21721 acccgcgctt ccgcgcgcgg ccggcgtggg cgcgcctggc gttgcgaaaa aaccacgacc
    21781 ccttgtattc ggccgccttg agaaccctat atctgggccg gctgggcggc gtcgactgct
    21841 gggccgccga gctcgccgcg gacgcgccgg cacccgcggg caccgcctgg gaagggctgc
    21901 ggccgctgtt ttcggtgctc gacgacgacc atttcgccct cgccggccgc gcgctgcagc
    21961 tcctcgaatg ggaccgcacc agccagttct gcggccgctg cggcacgccg accgaggcga
    22021 agcgcgagga gcgggtgcgc acctgtcccg cctgcaagct ttcggcctat ccgagggtcg
    22081 cgcctgccgt catggccctg gtcaagagag agaagcagat tctgctggcg cgcagcccgc
    22141 atttcccggc gggcatgtac agcgcgctcg ccggcttcgt cgagcccggc gagtcgctcg
    22201 agcagtgcct cgcgcgcgag gtggcggagg aagtcggcgt gcgcatctcc cgcgcgcgct
    22261 acttcgcgag ccagtcctgg ccgttcccgc attcgctcat gatcgcgttc gtcgccgagt
    22321 ggcagagcgg cgagatcaag ccgcaagacg gagaaataga agcggcaaac tggttcgaag
    22381 tattgcaatt gcctaaactt ccaagcaaaa tctcgatcgc tcgaagactg atcgatgcgg
    22441 ttgtcgacga tgtcaagggc aaaaacggtg tttaggaggt tttgaacgag ttgagcacga
    22501 tcgaagatcg ctgttttgcg ccgcaaggcg cgaatggaag gcgtggcctg gactcccaat
    22561 acgcggcagg ctgcgccttc gcattttgga gggtacggaa atgaacggtc tgcatctcat
    22621 cggtgacctc accggctgcc gctgcgatcc gcagctccta ctggacggcg agggctttcg
    22681 cgccaagtgc atcgagatgg tcgcggccgc cggcctcacg accatggatg ccaccttcca
    22741 caccttcgag ggcggcggct tcacgggcac cgtggtgctc gccgagtcgc accttgcgat
    22801 ccatacctgg cccgagcgcc aggggctgac gctcgacgtc tacgtctgca actacagcgc
    22861 ggataactcc gccaaggcgc gcaagctgtt cgacgtcctc gtcgactact tccagcccac
    22921 cgaggcggcg cgccacgaag tcgaccgcgg cgagcacctc ctgatggagc cgctcaacga
    22981 ctcgaccggc ttctacatca aggccagcaa gcagctcggc gaatggcaga cgcgtttcca
    23041 gaaaatggcg atctacgaga cgccgcacta cgggaagctg ttccgcctcg acgggttcaa
    23101 catgacctcc gagaaggagg agttcgtcta tcacgagaac ctgatccacc ccgcgctcac
    23161 cgcgcacgcc gcgccgaaga aggtcctcat catcggcggc ggcgacggcg gctcctccga
    23221 ggaggcgctc aagcacccct cggtcgagca ggtgaccatg gtcgagatcg acggcgacgt
    23281 gatcgaggtc gcgaagaccc atttccatgc cgtgcaccag ggcgtgttcg acaacccgaa
    23341 gctgcgcgtg ctcgtcgacg atggcatgaa attcgtgcgc gagacccagg agaagttcga
    23401 cctgatcgcg ctcgacctga acgacccgat gggcccggcc gaggccctct actcccccga
    23461 gttcttccag cagctgcgcc aggcactggc gcccggcgga gcgcttacgc tgcacatcgg
    23521 ctcgccggtg gcgcggccgg agcgggtcgc ccagctcgcc cagcggctga acacggtgtt
    23581 ccgcgtcgtg cggccctaca ccatgtacat cccgctctat ggctcgcagt gggcgatggc
    23641 ggtgtgctcg gacaagctcg atcccaagtc cttcaccgcc gacgagatcg accgccggat
    23701 cgaggcacgc aagctcaccg acctgcgcta ctacaacggc gagacccacg agggcgtgtt
    23761 cgcgctgccc aacttcgtcc gcgacctggt cgttccggcg cgcctgaagc agccggcgcg
    23821 cgggcggcgg ctgggcgtgg tggcagcggc taagtaagaa accccgctct agcgaccccc
    23881 ataccgctgt agcaggctga tcagggcggt ttccttgcgc tcgcgggcga tcttcatcgc
    23941 cgtgttgccg ccggacgcgg gacggttggg attggccccg gccttgagaa gcagctcggc
    24001 gctgacggcg tctcccatct gcacggcgag catcaggggc gtcagcccgg tgctgtcggg
    24061 cttgtccgcc cacttgccgt acctgagaag ctcctcgacc gcggacgagt cgccgtaaag
    24121 caccgcggtc gtgagatcgt tgaaccgcgg catgcgaggg gccatcggcc gcgccggcac
    24181 gagcaccggt ttgggcggtt gctcttccgc catgggagcc tccgcgggcg gtgccggttc
    24241 ggccttggca ggctcctttg gcgcagccgg cgcttgagcg accaccacgg gcgcactaac
    24301 cggagttacg accgccgggg ttgcgactgc cggggttaca accgccgggg tcggggttgc
    24361 gacaggagta ggagtagcta cggggacggt cttgggcgcc gccgaaggcc cgggtgcagg
    24421 gctcggcgcc gaggcggtcg cgatccccgg ctgcggcttg gcgaccggcg cggccggctt
    24481 gggcgtctgc acagcgactt ggggcccgct cagcatcgtg ccggcgattc cgccgaacac
    24541 gccggtgacc aggccggcgc cgaacatgaa caccgctacg aagggcgcaa gcagcattac
    24601 cgcagcgact gccagcgcgg ccgccacgtg gcccggctcg acgcgcacga cgcaggtcgt
    24661 ggcgatgaaa tcgtgcagcg cctgcttgcg gttggtgaag ccggcgatga tgaagccgag
    24721 aaggaaggtg aggaccgaca ggatcttcgc cacttcgcgg gcgagggagc gcccgaggct
    24781 gatgcgctcg ccgttgaggc cgccgacctt gaggccgacc agcgccttgc cgaaggtcgc
    24841 ctgccgcgcc gaggcctgca tgaaggtgta gtagaggacg cccatcgcga acaggatcag
    24901 ctcgttgctg atcagctcga gccccatcac gccaagcagc gccacgatcg cgaggaacgc
    24961 gatcgagacg atcaggccgt cgacgaggta cgcgaggaac cggatccaga agccgccgta
    25021 gttcatattg ttgttctccc gtcccttctt gttttcccgt ttcctgccgc ttagttcacc
    25081 gagcgcagca tcgtcctgat gcactcgaaa atgcggtcta tttgatcctt ttcgacgatg
    25141 agcggcggcg acagcgcgat cgtatcgccc gtgacgcgga tcagcacgcc cgcctcgaag
    25201 gccttcacga acacctcgta ggcgcgcttg cccggctggc cggggcgcgg ctcgagctcg
    25261 atcgcggcca tcagaccgca attgcggata tcggcgacgt gcggcgcccc cttgagggcg
    25321 tgcgccgtgt tttcccagtg cttctcgatc gtggaaaccc gggtgagcag gccctcttcc
    25381 tcgtaggtgt ccagcgtggc gacacccgcg gcgcaggcga gcggatggcc ggagtaggtg
    25441 tagccgtgga agagctcgat accctccgcg ccctgcatga acgcctcgaa gatcgagtcc
    25501 ttgatgaata cggcccccat cggcaccgtc gcgttcgtca ttcccttcgc ggtggtgaag
    25561 agatcgggca tgacgcccaa atgctgcgaa ccgaacggct ttccgaggcg gccgaacccg
    25621 gtgattacct cgtcgaagat gagcaggatc ccgtggcggg tgcagatctc gcgcagccgc
    25681 tcgaggtacc ccactggcgg caccagcacg cccgccgagc cggccaccgg ctcgacgatc
    25741 actgcggcga tggtcgacgg atcatgcagc aggcaaagcc gctcgagctc gtcggcgaac
    25801 tcctgtccgt gcttcggttg gccgcgcgaa aaggcgttcc tggcggggtc gtgcgtatgc
    25861 cggatatggt ccacccccgc cacgccgggc ccgaagaact tcttgttcgc ggtgatgccg
    25921 ccgaccgcca tgccgccgaa gttgaccccg tgatagccgc gctcgcgccc gatcagccgg
    25981 tagcggcccg cctcgccgcg cgcgcggtgg taggcaagcg cgatcttgag cgcggtgtcg
    26041 gccgattccg agcctgagtt ggtgaagaac acgcgcgaga agccctcggg cgcgatgcgc
    26101 gcaaggcgct cggcaaactc gaaggcgatc gggtgcccca tctggaaggt cggggcaaag
    26161 tccatctgcg cgacctggcg cttgaccgct tcgacgatct tcggccggca gtggccggcg
    26221 ttcacgcacc acaggcccgc ggtgccgtcg agcacctggc gcccttcggg cgtccagtag
    26281 tacatgccct cggcgcgggc gaggagccgc ggcgccttct tgaactgccg gttcgcggtg
    26341 aacggcatcc agaaggccgc ctgctcaagg ctgacttcct tcacttcggc acgcttgttc
    26401 atgccgcaga cattacacct tccgcgggga gcggttcacg ccgcaccgca gtactgcagc
    26461 ccggctagcc tgctaccccg agtatccgac caccttctgg cgctgctcgc ccaggccctg
    26521 gatgccgagg gtcatcaggt cgcccggctt gaggaacacc gggttgggct tgatcccaag
    26581 gccgacgccc ggcggcgtgc cggtggcgat gacgtcgccc ggcagcagcg tcatatagtg
    26641 gctcacgtgg gaaatgatct tcgccacgct gaacaccatg gtgcgcgtgt tgccggtctg
    26701 catgcgcttg ccgttcacgt cgagccgcat gtcgagcgtc tgcgggtcgg agatctcgtc
    26761 ggtcgtgacg agccaggggc cgatcggacc ggcggtgtcg aagcccttgc ccttgtccca
    26821 ctgggtggtc gccatctgga aggcgcgctc ggagacgtcg ttgatgatgc agtagccggc
    26881 gacgtggttc agcgcgtcgc tctcgctcac atacttcgcc tgcgtgccga taaccacgcc
    26941 gagctcgatc tcccagtcga gcttggtgga gttcttcggc tggatcacgt cgtcgctcgg
    27001 gccgttgatg caggtcgtgg ccttcatgaa aacgatcggc tcggtcggaa tcggcagccc
    27061 cgcctccttg gcgtgatcgg agtagttgag gccgatggcg acgaacttgc cgatgccgac
    27121 gtaaggcacg ccgaagcgcg ggctgccgtt taccggcggc aggctttccg gcttcaagcg
    27181 cgtgagcatt ttcagcccgc gcggcgagat ctcgttgggc ccgatgttgt cgatcacctt
    27241 ggagaggtcg cgcaggcgcc cctcgctgtc gatcaccccg ggcttttcgt agcccgcttt
    27301 tccgtagcgg cacagtttca tatcgtcatc cccccatcaa tcgaataagc gttgccggtc
    27361 gcgaacagcg actcgtccga agcgagaaac accaggatcc ccatgatgtc gtcgaccgtg
    27421 ccgagccgtc ccatcggctg gcgcgcgacg aagtccttgc gcgcctgcac cgggtcggca
    27481 aaggcgttga tccgtccctg gagcgacggg gtgtcgaccg tgcccgggca gatggcgttg
    27541 cagcggatcc ccttgcctac gtagtcggcg gcgatcgcct tggtcaagcc gatcaccgcg
    27601 gccttgctcg cgccgtagac gaagcggttc ggcaggccgc ggatcgagga
//
LOCUS       DATBSZ010000296        17228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2454336, whole genome shotgun sequence.
ACCESSION   DATBSZ010000296 DATBSZ010000000
VERSION     DATBSZ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17228
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2454336"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /gene="ndhC"
                     /locus_tag="VIG70_19465"
     CDS             <1..219
                     /gene="ndhC"
                     /locus_tag="VIG70_19465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY8252775.1"
                     /translation="EAFEDSRIKFDVRYYLVAILFIIFDLEIAFLFPWAVSLDAIGGF
                     GLLSMAVFLGVLVVGFIYEWKKGALEWD"
     gene            210..719
                     /locus_tag="VIG70_19470"
     CDS             210..719
                     /locus_tag="VIG70_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY8252776.1"
                     /translation="MGLTEAAPELAPVVQGRGFVTTTVDNLMNWARTGSLWPMTFGLA
                     CCAVEMMHAGAARYDLDRFGVVFRGSPRQSDVMIVAGTLVNKMAPALRKVYDQMPEPR
                     WVISMGSCANGGGYYHYSYAVVRGCDRIVPVDVYVPGCPPTAEALVYGILQLQAKIRR
                     TSTIARGPA"
     gene            716..1402
                     /locus_tag="VIG70_19475"
     CDS             716..1402
                     /locus_tag="VIG70_19475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY8252777.1"
                     /translation="MSRIEATRLALSARFGERTRPLPAIAGEIAIDVAAADLVAVCRE
                     LRDAPDLAFEQLIDLSGIDYLEYGRDEWATNEATATGFSRGVERGPRDEEPAGEGRFA
                     VSYQLLSVARNTRLRVRCCCPAGEPPMIDSVAEVWAAADWYEREAFDLYGILFRGHPD
                     LRRILTDYGFIGHPFRKDFPLSGRVEVRYDPSRGRVVYEPVSIEPRVTVPKVIRKERT
                     PGAPGSGGHV"
     gene            1395..2648
                     /locus_tag="VIG70_19480"
     CDS             1395..2648
                     /locus_tag="VIG70_19480"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY8252778.1"
                     /translation="MSEFQNFTLNFGPQHPAAHGVLRLVLEMDGEVIQKADPHIGLLH
                     RGTEKLAESKPFNQSIGYMDRLDYVSMMCNEHAYVRAIENLLGIAAPERAQYIRTMFD
                     EMTRILNHLMWIGSHGLDIGAMTVFLYAFREREDLMDCYEAVSGTRMHATYYRPGGVY
                     RDLPAKMPEYRESKYRSQGDLKRRNAARSGSLLDFVADFTDRFPACVDEYETLLTDNR
                     IWKQRTVNIGVVTPERALQLGFTGPMLRGSGVAWDLRKKQPYEVYDKVDFDIPVGKNG
                     DCYDRYLVRIEEFRQSNRIVRQCVDWLRKNPGPVMVDNHKIAPPSRGEMKLNMEELIH
                     HFKLFTEGMHVPEGEAYAAVEAPKGEFGIYLVSDGANKPYRLKIRPPGFAHLAALDEM
                     ARGHMLADAVAIIGTQDIVFGEIDR"
     gene            2648..3121
                     /gene="nuoE"
                     /locus_tag="VIG70_19485"
     CDS             2648..3121
                     /gene="nuoE"
                     /locus_tag="VIG70_19485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY8252779.1"
                     /translation="MLTTDSLRKIDREVAKYPPDQKQSAVMSALIIAQDEKGWLSTEI
                     MDYVAKVLGMPAVQVYEVASFYTMYNLKPTGRHKLTLCTCLPCGLQGALAAADHLRER
                     LRIDFNETTADGRFTLKEGECMGACAMAPVLLVNNKKMHDYMSNEKLDRLIEELK"
     gene            3121..4458
                     /gene="nuoF"
                     /locus_tag="VIG70_19490"
     CDS             3121..4458
                     /gene="nuoF"
                     /locus_tag="VIG70_19490"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY8252780.1"
                     /translation="MLDYGPDAILMEGLDGQNWRLADYEKRDGYAALKKILQEKIKPE
                     DLIADLKKSALRGRGGAGFPTGLKWSFMPRQFPGAKYVVCNSDEGEPGTCKDRDLLRY
                     NPHSVIEGMVIAGYAMAATAGYNYIHGEIWEVYEQFEEALKEAYAAGYVGKNVLGSDF
                     SFEMYAVPGYGAYICGEETALLESLEGKKGMPRFKPPFPASYGLYGKPTTINNTETFA
                     AVPWIVRNGGEAFLALGKPNNGGTKLFTVSGHVNRPGNYEVKLGTPFAKLLEMAGGVR
                     TGRKLKGVIPGGSSMPVLPAEIMMKTDMDYDSIAKAGSMLGSGAVIVMDDSTCMVRAL
                     ERLSYFYFEESCGQCTPCREGTGWLYRVVNRIETGRGRMEDLDLLDNVSDNIAGRTIC
                     ALGDAAALPVKSFIKHYRDEFVFHIQNKSCLHGTVEPRWGRSGSHLKTLQEAA"
     gene            4458..6644
                     /gene="nuoG"
                     /locus_tag="VIG70_19495"
     CDS             4458..6644
                     /gene="nuoG"
                     /locus_tag="VIG70_19495"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEY8252781.1"
                     /translation="MPKLIVDGTEVEVPHGATVMDATNKLGVYVPHFCYHRKLSIAAN
                     CRMCLVEVEKAPKPLPACATPVTDGMKVWTHSKLAKDAQNGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGGSNSRYTEEKRVVLRKELGPLVAAEEMSRCIQCTRCVRFGQEI
                     GGIMELGLAGRGENAEIVSFVGRAVDSELSGNMIDLCPVGALTSEPFRYKARTWELSR
                     RRSISPHDALGSNLVVQVKQNTVMRVLPLENEDINECWLSDKDRFSYDGLNSPDRLRR
                     PMVKQDGAWREVDWEQAFDFLVPRLQQEEVGVLASPHSTLEELFLVSRLGAADFRLRQ
                     SDFSADGKRGGIPWLGMPIAELGKLDRVLVVGSFLRKDHPLIAHRLRQAAKRGAQIHV
                     LHSVDDDWLMPIASRKIVAPSEIPGALASFGEVLKGGKNAAVLLGNFAQQHPQAAQIH
                     AAAQALGVRIGFLGEAANSVGGYLAGLPAGGNVGDVLKKKNLVLVNVEPELDCADPHA
                     VRHAFEVSNFVVSLTAFKTGLEYADALLPIAPFTETSGTFVSTEGRVQSFHAVVHPLG
                     DARPGWKVLRVLGSLLGRQGFDFDTVEQVRAACIAGRDVAGLLSNAIEVRATDTLEKV
                     QGIQRITDVPIYFADPLVRRAPPLQKTKDARPPRAWMNSRGLAKLGVAAGQPVLVNSS
                     VKLLAALDDRLPDDCVRVAAAHPTTVGVGAMFGTLTLSKAAAERAA"
     gene            6645..7721
                     /gene="nuoH"
                     /locus_tag="VIG70_19500"
     CDS             6645..7721
                     /gene="nuoH"
                     /locus_tag="VIG70_19500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY8252782.1"
                     /translation="MEWLALQSNAWLGPVWGPALYETGINLAFIAAIVAPLMGAVAYL
                     TLWERKFIGWVQIRIGPNRVGPAGLLQPVADGVKLLLKEIILPTNANKALYLLAPVLM
                     IMPALAAWAVIPFAPEVVLADINAGLLYIMALSSIGVYGVIVAGWASNSKYAFLGAMR
                     SAAQMVSYELAMGFALVVVLMVSGSLNLSDIVEGQARGWFASQGLTFLSWNWLPLLPM
                     LVVYFVSGVAETNRAPFDVVEGESEIVAGHMVEYSGMTFALFFMAEYMNMILISALTA
                     VMFFGGWQSPIDSVLLNWIPPFAWLLLKIFLIVTMFLWIRATFPRYRYDQIMRLGWKV
                     FIPLTLVWIAVIGIWMQTPWSLWK"
     gene            7732..8220
                     /gene="nuoI"
                     /locus_tag="VIG70_19505"
     CDS             7732..8220
                     /gene="nuoI"
                     /locus_tag="VIG70_19505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY8252783.1"
                     /translation="MTRLVDFFRSFLLLELMQGLRLTGRHLFARKITVQYPEEKTPAS
                     PRMRGLHALRRYPNGEERCIACKLCEAVCPALAIHIESEQRADGTRRTTRYDIDLVKC
                     IFCGFCEEACPVDAIVETRIVEYHGEKRGDLYYTKPMLLAVGDRYEESIARDRAQDAK
                     YR"
     gene            8248..8838
                     /locus_tag="VIG70_19510"
     CDS             8248..8838
                     /locus_tag="VIG70_19510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY8252784.1"
                     /translation="MFETWVFYALAAVLLVSGLLVITARNPVHGVLFLVLAFFTAAGI
                     WLLLRAEFLAITLVVVYVGAVMVLFLFVVMMLDINLERVREGFWRNLPLALVVGGIMV
                     LEMGFILAGRIWGVGVPKPVPAGYSNTKEIGRVLYTQYAYAFEIAAVLLLVAIIAAIA
                     LTLRKRKDSRSQDPSEQVRARREDRVRLVSMQSEKE"
     gene            8835..9143
                     /gene="nuoK"
                     /locus_tag="VIG70_19515"
     CDS             8835..9143
                     /gene="nuoK"
                     /locus_tag="VIG70_19515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY8252785.1"
                     /translation="MTLTLSHYLVLAAILFAISVVGIFLNRRNLIVLLMAIELMLLAV
                     NLNFIAFSHFLGDLAGQVFVFFILTVAAAESAIGLAILVVLFRNLNTIDVEDMDALKG
                     "
     gene            9158..11032
                     /gene="nuoL"
                     /locus_tag="VIG70_19520"
     CDS             9158..11032
                     /gene="nuoL"
                     /locus_tag="VIG70_19520"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY8252786.1"
                     /translation="MKTLYLIIAFAPLLAAIVAGLFGRQVGRVGAHSVTIFAVLLSFL
                     CSCVVFWDVLQGNHFNGPVYAWLTSGGVRFEIGFLIDPLTALMMVVVTFVSLMVHVYT
                     IGYMAEDPGYQRFFCYISLFTFAMLMLVMANNFLQLFFGWEAVGLVSYLLIGFWYTRE
                     SAVYANLKAFLVNRVGDLGFLLGIGLILAHFGTLDYVDVFRQAPQLQSGVTLICLLLF
                     VGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVARMSPLYELSETARS
                     VVLVIGAITALSMALVALTQYDIKRVVAYSTLSQLGYMTAALGASAYSAAMFHLMTHA
                     FFKAVLFLAAGSVIIALHHEQDMRRMGGLRKYMPITYWTMLIGAISSAGIPGFAGFFS
                     KDAIIEAVHHSATPGAMFAYVCVLVCVFVTAAYTFRLVFMAFHGPERFDKHHPPHESP
                     PVVTVPLVLLAIPSVLAGYAVGHVVFGDYFGSSLPAPDPNYYGLWSFIAHGLVSVPFW
                     LALAGIATAWFLYLKRTDLPKRIAQKLGPVYALVERKYGFDELYAWLFAAGARNLGRG
                     FWRAGDQALIDGLLVNGSARVVGWFSGVVRLFQSGFVYQYAFSMLIGVVILAFWFLKN
                     "
     gene            11033..12511
                     /locus_tag="VIG70_19525"
     CDS             11033..12511
                     /locus_tag="VIG70_19525"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY8252787.1"
                     /translation="MLLSLAIWLPIFAGVAVFAVGRNDGGSGAAAARWVALAGSVLGF
                     LVTLPLYTGFDIHNPGFQFELRQPWIERFNIWYHVGVDGISLLFVLLNSFITILVVIA
                     GWSVIDNRVGQYYGAFLVMSGLLNGVFAALDAVLFYVFFEGTLIPMFIIIGIWGGPNR
                     VYAAVKFFLYTLLGSLLMLVAMLYLYNKSGGSFSILDWHKLPLALGVQITLFFGFLLA
                     FAVKVPMWPVHTWLPDAHTEAPTGGSVVLAAILLKLGAYGFVRFTLPILPDASRHLAW
                     LMIALSLVAIAYIGLVTLVQTDMKRLIAYSSVSHMGFVTLGFFVFNAYGVEGGLIQMI
                     SHGFVSAAMFLCVGVLYDRMHTRMIGDYGGVVNTMPKFAAFAMLFAMANCGLPATSGF
                     VGEFLVIMGAMKASFWLALVAASTLVLGAAYTLWMYKRVIFGAVANSHVAALKDLNFR
                     EFIVLGALAFAVLFMGVYPLPFTEVMHASVSELLRHVAAPKL"
     gene            12519..14006
                     /gene="nuoN"
                     /locus_tag="VIG70_19530"
     CDS             12519..14006
                     /gene="nuoN"
                     /locus_tag="VIG70_19530"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY8252788.1"
                     /translation="MNWIAIIDHLPALPEAILLLGACALMIVDLYVKGAERRATLWLA
                     QAVLALCVCATLFVLWGSGGRRLLIFSGLFVADQMGHLLKLVCYLAVSAALVYSRQYL
                     QDRALLRGEFVTLLLFSLLGMMVMISAASFLTLFLGLELMSLCLYALVALNRDSALST
                     EAAMKYFVLGALSSGLLLYGMSMIYGATGTLNLYEVAARSARLVGNESDRVFLVFGLG
                     FVVAGLAFKVGVVPFHMWIPDVYQGAPTAVTLIVGSAPKVAAFAMAVRLLVNGLPDLA
                     RDWQQMLALLAIASMAIGNITAIAQTNLKRMLAYSTIAHMGFMLLGLLSGVVDGNRLS
                     ADGAYSSALFYMIVYVLMSLGAFGVLLLLSRAGFECENLDDVKGLNRTHPWIAFIMLL
                     LMFSLAGIPPTAGFYAKLAVLSAAVDAGQIWLAVAAVILSLIGAFYYLRVVKLMYFDD
                     PKVPPQVTGSLGFRVALSANGLAMLLFGILPSLLMIPCLEAIKAL"
     gene            13984..14538
                     /locus_tag="VIG70_19535"
     CDS             13984..14538
                     /locus_tag="VIG70_19535"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8252789.1"
                     /translation="MKRSKRSDLTEHFVAGSRVFDGNLLKVHRDTVRLPDGSEGSREY
                     IRHPGAVTIVALFEDGKVLLERQFRYPSRREFIELPAGKIDPGEPHLDTAKRELLEET
                     GYTASDWTRLGVIHTSIGYSDEAIELFRATGLEHRGAQLDAGEFLEIFSVPFEEAIAM
                     VGDGRITDAKSVAGLLWLKTFAPV"
     gene            14550..15245
                     /locus_tag="VIG70_19540"
     CDS             14550..15245
                     /locus_tag="VIG70_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY8252790.1"
                     /translation="MTELRRSAERGHANHGWLDSYHSFSFADYYDPKHMGFGPLRVIN
                     EDRVQPGKGFGTHGHRDMEIISYVLEGALGHQDSMGNGSTIVPGDVQRMSAGSGVRHS
                     EYNHEKQGATHFLQIWIEPNVTGIPPSYEQKHFTPADKRGRLRLIASPDGRDGSVTLH
                     QDALVCAGLFDGRENASYELRNERGYVHVARGRITVNGHVLEAGDALKTEDGVIELEN
                     GEQAEVLVFDLPE"
     gene            complement(15261..16040)
                     /locus_tag="VIG70_19545"
     CDS             complement(15261..16040)
                     /locus_tag="VIG70_19545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8252791.1"
                     /translation="MALVYYLTPAGKDALREKDGAAKLPLEYRRLLALMELEGHIDVI
                     RGRLRRFPDRLIEEWLKELEDLKMIEPRWPAGKAADITFSGSRVPQLPPLPDEDSLRF
                     AKTTVIAGATLLRSGSFLAVDRIANLPALAKAPAKTVVLLVEDDPDQMALGELRLQMA
                     GYQVRSVDRAKALSRFLREQARPDLLLLDVMLPDGNGFDILSRLRARPEFAMLPIVLL
                     TVKAELADIRKGLVLGADGYITKPYSKNQLAEVIGRVLKQT"
     gene            complement(16141..>17228)
                     /locus_tag="VIG70_19550"
     CDS             complement(16141..>17228)
                     /locus_tag="VIG70_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HEY8252792.1"
                     /translation="QDDVNRRIQVKSVPAPVLASLRWPGRPDLSGGNPAWTYMIEHPQ
                     YRFAVFVGHVENGSAAPGSAPHSGGRPHAFEVWVNGSEQPRGLGALAKTLSMDMRADD
                     SAWLKLKLDTLAKAGGDEPFEMPFPPRGEKKLMPSVVSAFAQIVRWRVEQLQAGLAGD
                     EFQLAGEEASSPVLDAMFALKEPKTGTDGTMSWTVDVLNPRTGDDFVLGLKEITLPDG
                     ITRPYSVWLSGEYPRALDGLCKILSLDMRVLDPAWVGMKLKKLLEFPEPLGDFMAFVP
                     GSRKQQTFPSTVSYLARLILHRYAMLGILDDVGSAKQQMGILEAPAVKSTPVIAGARC
                     PECANPTLIRKDGCDYCTACGYVGVCG"
BASE COUNT         2718 a         5700 c         5680 g         3130 t
ORIGIN      
        1 gaggccttcg aggactcgcg catcaagttc gacgtgcgct attacctggt cgcaatcctc
       61 ttcatcattt tcgacctcga gatcgccttc ctgtttccgt gggcggtgtc gctcgacgcg
      121 atcggcggct tcggcctcct gtcgatggcc gtctttctcg gcgtcctcgt ggtcggcttc
      181 atctacgaat ggaagaaagg ggcgctcgaa tgggactgac ggaagccgcc ccggaactcg
      241 cgcccgtcgt ccagggccgc ggattcgtga ccacgacggt cgacaacctc atgaactggg
      301 cgcgtaccgg gtccctctgg cccatgacct ttggcctcgc ctgttgcgcc gtcgaaatga
      361 tgcacgcggg agcggcacgc tacgacctcg accgcttcgg cgtcgtcttc cgcggcagcc
      421 cgcgccagtc cgacgtgatg atcgtcgcgg gaacgctcgt caacaagatg gcgccggcgc
      481 tgcgcaaggt ctacgaccag atgccggagc cgcgctgggt catctccatg ggctcctgcg
      541 cgaacggagg cggctactac cactattcct atgccgtcgt gcgcggctgc gaccgtatcg
      601 tccccgtcga cgtctacgtg ccgggctgcc ccccgaccgc ggaagcgctc gtctacggca
      661 tcctgcagct gcaggccaag atccgccgca ccagcaccat cgcgcgcggt cccgcatgag
      721 ccgcatcgag gcgacgcggc tggccctgtc cgcccggttc ggcgagcgca cccggccgct
      781 gcccgcaatc gcgggcgaga tcgcgatcga cgtggcggcg gcggacctcg tcgccgtgtg
      841 cagggaattg cgcgacgcgc cggacctcgc cttcgagcag ctcatcgacc tctcggggat
      901 tgactacctc gaatacggcc gcgacgagtg ggccacgaac gaggcgacag cgactggctt
      961 cagccgcggc gtcgagcgcg gcccgcgcga tgaggagccc gccggcgagg gccgcttcgc
     1021 cgtctcctac caactcctgt ccgtcgcgcg gaacacgcgg ctgcgcgtcc gttgctgctg
     1081 cccggccggc gagccgccga tgatcgattc ggtggcggaa gtctgggctg cggccgactg
     1141 gtacgagcgc gaggccttcg acctctacgg catcctgttc cgcggccatc cggacctgcg
     1201 gcgcatcctc accgactacg gcttcatcgg ccacccgttc cgcaaggact tcccgctgtc
     1261 cggccgcgtc gaagtccgct acgacccctc gcggggccgc gtcgtttacg aaccggtcag
     1321 catcgagccg cgagtcaccg tgccgaaggt gattcgcaag gaacgcacgc cgggtgcgcc
     1381 agggtcgggc ggccatgtct gagttccaga acttcacgct gaacttcggc ccgcagcacc
     1441 ccgcggccca cggcgtgctc cgcctcgtgc tcgagatgga cggcgaggtg atccagaaag
     1501 ccgacccgca tatcggactc ctgcaccgcg ggaccgagaa actcgccgag tcgaagccgt
     1561 tcaaccagtc gatcggctac atggaccggc tcgactacgt gtcgatgatg tgcaacgagc
     1621 acgcctacgt gcgcgcgatc gagaacctgc tcggcatcgc cgcaccggag cgtgcccagt
     1681 acatccgcac gatgttcgac gagatgaccc gcatcctgaa ccacctgatg tggatcggct
     1741 cgcacggcct cgacatcggc gcgatgacgg tcttcctgta cgcgttccgc gagcgcgagg
     1801 acctcatgga ctgctacgag gcggtctcgg gcacgcgcat gcacgcgacg tactaccggc
     1861 cgggcggggt ctaccgcgac ctgccggcga agatgccgga gtaccgggag tcgaagtacc
     1921 gcagccaggg tgacctgaag cgcaggaacg cggcgcgctc gggctcgctc ctcgacttcg
     1981 tggcggactt cacggaccgc ttcccggcct gcgtggacga gtacgagacg ctgctgaccg
     2041 acaaccgcat ctggaagcag cgcacggtca acatcggtgt ggtcacgccc gagcgcgcgc
     2101 tgcagcttgg cttcaccggg ccgatgctgc gggggtcggg cgtcgcctgg gacctgcgca
     2161 agaagcagcc ctacgaggtc tacgacaagg tcgacttcga catcccggtc gggaagaacg
     2221 gcgactgcta cgaccggtat ctcgtgcgca tcgaggaatt ccgccagtcg aaccgcatcg
     2281 tcaggcagtg cgtcgactgg ctgcgcaaga accccgggcc ggtgatggtc gacaaccaca
     2341 agatcgcgcc gccctcgcgc ggcgagatga agctcaacat ggaagagctg atccaccact
     2401 tcaagctctt caccgagggc atgcacgttc ccgaaggcga ggcctacgcc gcggtcgagg
     2461 cgcccaaggg cgagttcggc atctacctcg tctcggacgg cgcgaacaag ccctaccgcc
     2521 tgaagatccg ccctcccggc ttcgcccacc tcgccgcgct cgacgagatg gcgcgcggcc
     2581 acatgctcgc ggacgcggtg gcgatcatcg gaacgcagga catcgttttt ggcgaaatcg
     2641 acaggtaatg ctgacgacgg attctctgag gaagatcgac cgcgaggtcg cgaagtaccc
     2701 gccggaccag aagcagtcgg cggtgatgag cgcgctcatc atcgcgcagg acgagaaggg
     2761 ctggctttcc accgagatca tggactacgt ggcgaaggtc ctcggcatgc cggcggtgca
     2821 ggtctacgag gtggcgagct tctacaccat gtacaacctc aagccgaccg ggcgccacaa
     2881 gctcacgctg tgcacctgcc tgccgtgcgg gctgcagggc gcgctcgccg cggcggacca
     2941 cctgcgcgag cggctgcgca tcgacttcaa cgaaaccacg gccgacgggc gctttaccct
     3001 caaagagggc gagtgcatgg gggcgtgcgc gatggcgccg gtgctgctcg tcaacaacaa
     3061 gaagatgcac gactacatgt cgaacgaaaa gctcgaccgc ttgatcgagg aactgaagtg
     3121 atgctggatt acggacccga cgcgatcctg atggagggcc tcgacggcca gaactggcgg
     3181 cttgccgatt acgagaagcg cgacggttac gcggcgctga agaagattct ccaggaaaag
     3241 ataaaaccgg aagatctgat cgcggacctc aagaagtccg cgctgcgcgg ccgcggcggc
     3301 gcggggtttc cgaccgggct caagtggtcg ttcatgccgc gccagttccc cggcgcgaag
     3361 tacgtggtgt gcaattccga cgagggcgag ccgggcacct gcaaggaccg cgacctcctg
     3421 cgctacaacc cgcactcggt gatcgagggc atggtgatcg ccgggtatgc gatggccgcg
     3481 accgccggct acaactacat ccacggcgag atctgggaag tctacgagca gttcgaggaa
     3541 gcgctgaagg aggcctacgc ggcggggtat gtcggcaaga acgtcctggg ttctgatttt
     3601 tcgtttgaaa tgtacgccgt cccgggctac ggggcgtaca tctgcggcga ggagacggcg
     3661 ctgctcgagt cgctcgaggg caagaagggc atgccgcgct tcaagccgcc gttcccggcg
     3721 agctacggcc tgtacggcaa gccgaccacc atcaacaaca ccgagacctt cgccgcggtg
     3781 ccctggatcg tgcgcaatgg cggcgaggcg ttcctcgcgc tcggcaagcc gaacaacggc
     3841 ggtaccaagc tcttcaccgt cagcggccat gtcaaccggc cgggcaacta cgaggtgaag
     3901 ctcggcacgc cgttcgcgaa gcttctcgag atggcgggcg gcgtgcgcac cggccgcaag
     3961 ctcaagggcg tgatcccggg cggttcctcg atgccggtgc tgccggccga gatcatgatg
     4021 aagaccgaca tggattacga ctcgatcgcc aaggcgggct cgatgctcgg ctcgggcgcg
     4081 gtgatcgtga tggacgactc gacctgcatg gtgcgcgcgc tcgagcggct gtcctacttc
     4141 tacttcgagg agtcctgcgg gcagtgcacg ccgtgccgcg agggcacggg gtggctctat
     4201 cgggttgtga accgcatcga gaccggccgc ggccgcatgg aggacctgga cctcctcgac
     4261 aacgtctcgg acaacatcgc cggccgcacc atctgcgcgc tgggcgacgc cgcggcgctg
     4321 ccggtgaaaa gcttcatcaa gcattaccgg gatgagttcg tttttcatat tcagaacaaa
     4381 tcctgtttgc acggcacggt ggagccgcgc tggggccgct ccggctccca cttgaaaacg
     4441 ctgcaagagg ccgcctgatg ccgaagctga tcgtcgacgg caccgaagtg gaggtcccgc
     4501 acggcgcgac cgtgatggac gccaccaaca agctcggcgt ctacgtgccg cacttctgct
     4561 atcaccgcaa gctctcgatc gccgccaact gccgcatgtg cctggtcgag gtcgagaagg
     4621 cgcccaagcc gctgccggcc tgcgccacgc cggtcaccga cggcatgaag gtctggaccc
     4681 actccaagct ggcgaaggac gcgcagaacg gggtgatgga gttcctcctc atcaaccacc
     4741 cgctcgactg cccgatctgc gaccagggcg gcgaatgcca gctgcaggac ctcgcggtcg
     4801 gctacggcgg cagcaactcg cgctacaccg aagagaagag agtcgtcctc agaaaagagc
     4861 tcggcccgct ggtggcggcg gaagagatga gccgctgcat ccagtgcacg cgctgcgtgc
     4921 gcttcggcca ggagatcggc ggcatcatgg agctcggcct cgccggtcgc ggcgagaacg
     4981 ccgagatcgt gtcgttcgtc ggccgcgcgg tcgattcgga gctctccggc aacatgatcg
     5041 acctgtgccc ggtgggcgcg ctcacctcgg agccgttccg ctacaaggcg cgcacctggg
     5101 agctctcgcg ccgccgctcg atctcgccgc acgacgcgct cggctcgaac ctggtggtgc
     5161 aggtgaagca gaacaccgtg atgcgcgtcc tgccgctcga gaacgaagac atcaacgagt
     5221 gctggctctc ggacaaggac cgcttctcct acgacggcct gaactcgccc gaccggctgc
     5281 gccgcccgat ggtcaagcag gacggcgcgt ggcgcgaggt cgactgggag caggcgttcg
     5341 acttcctggt gccgcggctg cagcaggagg aggtcggcgt gctcgcgtct ccgcactcga
     5401 cgctggaaga attgttcctg gtttcaagat taggtgcggc cgacttccgc ctgcggcaaa
     5461 gcgacttctc ggccgacggc aagcgcggcg gcatcccctg gctcggcatg ccgatcgccg
     5521 agctgggcaa gctcgaccgg gtgctcgtgg tcggctcgtt cctgcgcaag gaccacccgc
     5581 tgatcgcgca ccgcctgcgc caggccgcca agcgcggcgc gcagatccac gtcctgcact
     5641 cggtggacga cgactggctg atgccgatcg cgagcaggaa gatcgttgcg ccatcggaga
     5701 ttcccggcgc attggcttct tttggagaag tgctgaaagg cgggaaaaac gccgcggtcc
     5761 tcctcggcaa cttcgcgcag cagcatcccc aggcggcgca gatccacgcc gccgcccagg
     5821 cgttgggcgt gagaatcggt ttccttggcg aagcggcgaa ctccgtgggc ggctacctgg
     5881 cgggcctgcc ggcgggcggg aacgtcggcg acgtcctgaa gaagaaaaac ctggtgctgg
     5941 tgaacgtgga gcccgagctc gactgcgccg atccgcacgc ggtgcgccat gcgttcgaag
     6001 tgtccaactt cgtggtcagc ctgacggcct tcaagaccgg gctcgagtac gccgacgcgc
     6061 tgctgccgat cgcgccgttc accgagacct cgggcacctt cgtcagcacc gagggccgcg
     6121 tccagagctt ccacgccgtg gtgcacccgc tcggcgacgc gcgcccgggc tggaaggtgc
     6181 tgcgcgtgct gggcagcctg ctcggccggc agggcttcga tttcgacacc gtcgagcagg
     6241 ttcgggcggc gtgcatcgcg ggcagggacg tcgcggggct gctttcgaac gcgatcgaag
     6301 ttcgagcaac agatactctt gaaaaagtcc aaggcatcca acgcatcacg gacgtgccga
     6361 tctacttcgc cgatccgctg gtgcgccgcg cgccgccgct gcagaagacg aaggacgcgc
     6421 ggccgccgcg ggcgtggatg aactcccgcg ggctggcgaa gctcggcgtc gccgcggggc
     6481 agccggtgct ggtgaattct tcggtcaagc tgttggccgc gctcgacgac aggctgcccg
     6541 acgattgcgt gcgcgtcgca gcggcgcatc cgacaaccgt cggggtgggc gcgatgttcg
     6601 ggacattgac gctgtcgaag gcggccgcgg agcgggcggc ctagatggag tggctcgcgc
     6661 tccagtcgaa cgcctggctc ggcccggtct ggggaccggc gctctacgag accggcatca
     6721 acctcgcctt catcgccgcc attgtggcgc cgctcatggg cgcggtcgcc tatctcacgc
     6781 tgtgggagcg caagttcatc ggctgggtgc agatccgcat cgggccgaac cgcgtcgggc
     6841 cggcgggcct gctgcagccc gtcgccgacg gcgtcaagct cctcctcaag gagatcatcc
     6901 tccccaccaa cgccaacaag gcgctctatc tcctggcgcc ggtgctgatg atcatgccgg
     6961 cgctcgccgc ctgggcggtc atcccgttcg cgcccgaggt ggtgctcgcc gacatcaacg
     7021 cggggctcct gtacatcatg gcgctctcct cgatcggggt gtacggcgtg atcgtcgccg
     7081 gctgggcgtc gaactcgaag tacgccttcc tcggcgcgat gcgctcggcg gcgcagatgg
     7141 tctcctacga gctcgcgatg ggcttcgcgc tggtggtggt gctgatggtc tcggggagcc
     7201 tcaacctctc cgacatcgtc gaggggcagg cccggggctg gttcgcctcg cagggcctca
     7261 cgttcctgtc gtggaactgg ctgccgctcc tgcccatgct ggtggtgtac ttcgtgagcg
     7321 gcgtcgccga gaccaaccga gcgccgttcg acgtggtcga aggcgagtcc gagatcgtcg
     7381 ccggccacat ggtcgagtac tcggggatga cgttcgcgct gttcttcatg gccgagtaca
     7441 tgaacatgat cctgatcagc gcgctcacgg cggtcatgtt cttcggcggc tggcagtcgc
     7501 ccatcgattc ggtgctgctg aactggattc cgccgttcgc gtggctgctt ctgaagatct
     7561 tcctgatcgt gacgatgttc ctgtggatcc gcgccacgtt cccgcgctac cgctacgacc
     7621 agatcatgcg cctgggctgg aaggtgttca ttccgctcac gctggtatgg atcgcggtga
     7681 tcggcatctg gatgcagacg ccgtggagcc tgtggaaatg agcccgcatc tatgacccgt
     7741 ctggtcgatt ttttcaggtc ctttctcctg ctcgagctga tgcagggcct gcgccttacc
     7801 ggccggcatc tcttcgcgcg caagatcacg gtgcagtacc cggaagagaa gacgccggcg
     7861 agcccgcgca tgcgcggcct gcatgcgcta cgccgctatc cgaacggcga ggagcgctgc
     7921 atcgcctgca agctgtgcga ggcggtgtgc ccggcgctcg cgatccacat cgaatccgag
     7981 cagagagccg acggaacgcg ccgcaccacg cgctacgaca tcgacctggt gaagtgcatc
     8041 ttctgcggct tctgcgaaga ggcctgcccg gtggacgcga tcgtcgagac ccgcatcgtc
     8101 gagtaccacg gcgagaagag gggggatctc tactacacca agcccatgct gctcgcggtg
     8161 ggcgaccgct acgaggagag catcgcccgc gaccgggcgc aagacgcgaa gtaccgataa
     8221 ccgacaaccc ataatcatca aaaaatagtg ttcgaaacct gggtcttcta cgcgctcgcc
     8281 gcggtattgc tcgtgtccgg cctgctcgtc atcacggcgc gcaacccggt gcacggggtg
     8341 ctgttcctgg tgctcgcgtt cttcaccgcc gcggggatct ggctgctcct gcgcgccgag
     8401 tttctcgcca tcacgctggt ggtggtctac gtcggcgccg tgatggtgct gttcctgttc
     8461 gtggtgatga tgctcgacat caacctcgag cgcgtgcgcg agggcttctg gcgcaacctg
     8521 ccgctcgcgc tggtggtggg cggcatcatg gtgctcgaaa tgggattcat ccttgcgggg
     8581 cggatctggg gggttggcgt gccaaagccg gttcccgccg gctacagcaa caccaaggag
     8641 atcgggcgcg tcctgtacac ccagtatgcg tacgccttcg agatcgccgc cgtgctcctg
     8701 ctcgtcgcca tcatcgccgc gatcgcgctc actctgcgca agcgcaagga cagccgttcg
     8761 caggacccgt cggagcaggt gagggcgagg cgcgaagaca gagtccggct ggtctcgatg
     8821 cagtccgaga aagaatgacc cttacgcttt cccattacct ggtcctcgcc gcgatcctgt
     8881 tcgcgatcag cgtggtgggc atcttcctca accgccgcaa cctgatcgtg ctgctgatgg
     8941 cgatcgagct gatgctgctc gcggtcaacc tcaatttcat cgccttctcg catttcctcg
     9001 gcgaccttgc cgggcaggtg ttcgtgttct tcattctcac ggtggcggcg gcggagtcgg
     9061 ccatcggctt ggcgatcctg gtggttcttt tccggaacct gaacaccatt gacgtcgaag
     9121 atatggacgc cctaaaggga taaaagcaag acacgggatg aaaaccctgt acctcatcat
     9181 cgcgttcgcg ccgctgctgg cggcgatcgt cgcgggactc ttcggccggc aggtgggccg
     9241 cgtcggcgcg cattcggtga cgatcttcgc ggtgctgctc tcgttcctgt gctcgtgcgt
     9301 ggtgttctgg gacgtgctcc agggcaacca cttcaacggc ccggtctacg cctggctcac
     9361 gagcggcggg gtgcgcttcg agatcggctt cctgatcgac ccgctgaccg cgctgatgat
     9421 ggtggtggtc accttcgtgt cgctgatggt gcacgtctac accatcggct acatggccga
     9481 ggacccgggc taccagcgct tcttctgcta catctcgctc ttcaccttcg ccatgctgat
     9541 gctggtgatg gcgaacaatt tcctgcagct gttcttcggc tgggaggcgg tcgggctggt
     9601 gtcgtatctg ctgatcgggt tctggtacac gcgcgagagc gcggtctacg ccaatctcaa
     9661 ggcgttcctc gtcaaccgcg tcggcgacct cggcttcctc ctcggcatcg ggctgatcct
     9721 cgcgcacttc gggacgctcg actacgtcga cgtgttccgg caggcgcccc agctccagtc
     9781 gggcgtgacc ctgatctgcc tcctgctgtt cgtgggcgcg atgggcaaga gcgcgcagtt
     9841 cccgctgcac gtctggctgc ccgactcgat ggaaggcccg acgccgatct ccgcgctgat
     9901 ccacgcggcc accatggtga ccgcgggcat cttcatggtc gctcggatgt cgccgttgta
     9961 cgaattgagc gaaacggcaa gatcggtcgt gctggtcatc ggtgcaatca ccgcgctctc
    10021 gatggcgctg gtcgcgctca cgcagtacga catcaagcgc gtggtggcgt actcgaccct
    10081 ctcgcagctc ggctacatga ccgcggcgct cggcgcgtcg gcgtactcgg cggcgatgtt
    10141 ccatctcatg acgcacgcct tcttcaaggc ggtgctgttc cttgccgccg gctcggtgat
    10201 catcgcgctg catcacgagc aggacatgcg ccgcatgggc ggcctgcgca aatacatgcc
    10261 catcacgtac tggacgatgc tgatcggcgc gatctcgtcc gccggcatcc ccggctttgc
    10321 cggcttcttc tccaaggacg ccatcatcga ggcggtgcac cattcggcca cgcccggggc
    10381 gatgttcgcc tacgtctgcg tcctcgtctg cgtgttcgtc accgcggcgt acaccttccg
    10441 cctggtgttc atggcgttcc acgggcccga gcgcttcgac aagcaccacc cgccgcacga
    10501 gtcgccgccc gtggtcacgg tgccgctggt cctgctcgcc atcccttccg tccttgccgg
    10561 atacgcggtc gggcacgtgg tgttcggcga ctatttcggc tcgagcttgc cggcccccga
    10621 cccgaactat tacggccttt ggtcgttcat cgcgcacggc ctggtgtcgg tgccgttctg
    10681 gctggcgctg gccgggatcg ccactgcctg gttcctgtac ctgaaaagaa cggacctgcc
    10741 gaagcgcatc gcgcagaagc tcgggccggt ctatgcgctg gtcgagcgca agtacggctt
    10801 cgacgagctc tacgcctggc tgttcgccgc cggcgcgcgc aatctcggca ggggcttctg
    10861 gcgcgccggc gaccaggcgc tgatcgatgg cctgctggtg aacgggtcgg cgcgcgtggt
    10921 cggctggttc tcgggcgtgg tgcgcctgtt ccagagcggc ttcgtctacc agtacgcgtt
    10981 cagcatgctg atcggcgtgg tgatcctcgc tttctggttc ctgaaaaact aagtgctgct
    11041 ttccctcgcc atctggctgc ccattttcgc cggcgttgcg gtgttcgccg tcgggagaaa
    11101 tgacggcggc tcgggcgccg ccgcggcgcg ctgggtggcg ctcgcaggca gcgtgctcgg
    11161 ttttctcgtc accctcccgc tctacaccgg cttcgacatc cacaacccgg gcttccagtt
    11221 cgagctgagg cagccctgga tcgagcgctt caacatctgg taccacgtcg gcgtcgacgg
    11281 catctcgctc ttgttcgtcc tcctgaacag tttcatcacc atcctcgtgg tgatcgccgg
    11341 ctggtcggtg atcgacaacc gcgtcggcca gtactacggc gccttcctcg tcatgtcggg
    11401 cctgctgaac ggggtgttcg cggcgctcga cgcggtgctg ttctacgtgt tcttcgaagg
    11461 cacgctgatc ccgatgttca tcatcatcgg catctggggc gggccaaacc gggtgtacgc
    11521 ggcggtgaag ttcttcctct acacgctgct cgggtcgctg ctgatgctgg tggcgatgct
    11581 gtacctctac aacaagtcgg gcggcagctt ctcgatcctc gactggcaca agctgcccct
    11641 cgcgctcggc gtgcagatca cgctgttctt cggcttcctg cttgcctttg cggtcaaggt
    11701 gccgatgtgg ccggtgcaca cctggctgcc ggacgcccac accgaggcgc cgaccggcgg
    11761 gtcggtggtg ctggcggcga tcctgctgaa gctgggcgcg tacggcttcg tgcgcttcac
    11821 gctgccgatc ctgcccgacg cctcgcgcca cctcgcgtgg ctcatgatcg cgctctcgct
    11881 ggtggccatc gcgtacatcg gcctggtcac cctggtgcag accgacatga aacgcctgat
    11941 cgcctattcg tccgtctccc acatggggtt cgtcacgctc gggttttttg tcttcaatgc
    12001 ttatggcgtc gaaggcggcc tgatccagat gatctcgcac ggcttcgtgt cggcggcgat
    12061 gttcctttgc gtgggggtgc tctacgaccg catgcacacg cgcatgatcg gcgactacgg
    12121 gggcgtggtg aacaccatgc cgaagttcgc cgcgttcgcc atgctcttcg cgatggccaa
    12181 ttgcggcctg ccggcgacca gcggcttcgt cggcgagttc ctggtgatca tgggcgcgat
    12241 gaaagcgagc ttctggctcg cactcgtcgc cgccagcacg ctggtgctcg gcgcggccta
    12301 cacactgtgg atgtacaagc gcgtgatctt cggcgccgtg gcgaatagcc acgtcgctgc
    12361 attgaaagac ctgaatttca gagaattcat cgtgctcggg gccctcgcat tcgccgttct
    12421 gttcatgggc gtctatccgc tgcccttcac cgaggtgatg cacgcctcgg tgagcgagct
    12481 gttgcgccac gtggcggcgc ccaagctgtg agcccggcat gaactggatc gcgatcatcg
    12541 atcacctgcc ggcgctgccc gaggcgatcc tgctcctcgg cgcatgcgcg ctgatgatcg
    12601 tcgacctcta cgtcaagggc gccgagcgcc gcgccacgct ctggctcgcg caggccgtcc
    12661 tcgcgctgtg cgtgtgcgcg accttgttcg tgctgtgggg ctcgggcggc cggcgcctgc
    12721 tcatcttcag cgggctgttc gtcgccgacc agatgggcca cctgctgaag ctggtgtgct
    12781 atctcgcggt atcggcggcg ctggtgtatt cgcgccagta cctgcaggat cgcgcgctgc
    12841 tgcgcggcga gttcgtgacg ctgctgctgt tctcgctgct cggcatgatg gtgatgatca
    12901 gcgcggcgag cttcctgacg ctgttcctcg gcctcgagct gatgtcgctc tgcctgtacg
    12961 cgctggtcgc gctgaaccgc gactccgccc tctccaccga ggcggcgatg aagtacttcg
    13021 tgctcggcgc gctgtcctcg ggcctgctgc tctacggcat gtcgatgatc tacggcgcca
    13081 ccggcacgct caacctctac gaggtagcgg cccggtccgc ccggctggtc ggcaacgagt
    13141 ccgaccgcgt gttcctggtg ttcggcctgg ggttcgtggt cgccggcctc gcgttcaagg
    13201 tcggcgtggt gccgttccac atgtggattc ccgacgtcta ccagggcgcg cccaccgcgg
    13261 tgacgctgat cgtcggctcg gcgccgaagg tcgccgcgtt cgccatggcg gtgcgcctgc
    13321 tggtgaacgg gctgcccgac ctggcgcgcg actggcagca gatgctcgca ctgctcgcga
    13381 tcgcctcgat ggcgatcggc aatatcaccg cgatcgcgca aacgaacctg aagcgcatgc
    13441 tggcctactc gaccatcgcg cacatgggct tcatgctgct cgggctgctc tcgggcgtgg
    13501 tcgacggcaa tcggctgagc gccgacggcg cctactcgag cgcgctgttc tacatgatcg
    13561 tgtacgtgtt gatgtcgctc ggcgccttcg gcgtgctgct cctgctgtcg cgcgccggct
    13621 tcgagtgcga gaacctcgac gacgtaaaag gcctcaaccg cacccacccc tggatcgcgt
    13681 tcatcatgct gctcttgatg ttctcgctcg ccggcatccc gcccactgcc gggttctacg
    13741 ccaagctcgc cgtgctttcg gccgcggtag acgcgggcca gatctggctc gccgtagcgg
    13801 cagtgatcct gtcgctgatc ggcgcgttct actacctccg cgtggtgaag ctcatgtatt
    13861 tcgacgatcc gaaggttccg ccgcaggtga ccggcagcct gggcttccgc gtcgcgctct
    13921 ccgccaacgg cctggcgatg ctgctgttcg gcatcctgcc ctcgctcctg atgatcccct
    13981 gtcttgaagc gatcaaagcg ctctgacctg accgagcact tcgtcgccgg gagccgcgtg
    14041 ttcgacggca acctgctcaa ggtgcaccgc gacaccgtgc ggctgcccga cggcagcgaa
    14101 gggagccgcg aatacatccg ccaccccggc gcggtgacga tcgtcgcgct gttcgaggac
    14161 ggcaaagtgc tgctcgagcg ccagttccgc tatccgagcc ggcgggagtt catcgagctg
    14221 ccggcgggca agatcgaccc cggcgagccg cacctcgaca ccgcgaagcg cgagctgctc
    14281 gaggaaaccg gctacaccgc ctccgactgg acccggctcg gcgtgatcca cacctcgatc
    14341 ggctacagcg acgaggcgat cgagcttttc cgggccacgg gactggagca ccggggggcg
    14401 cagctcgacg cgggcgaatt cctcgagatc ttcagcgttc ccttcgagga agccatcgcc
    14461 atggtcggcg acggccgcat cacggatgca aagagcgtgg ccggcctgct ctggctgaaa
    14521 acctttgcgc cggtgtagca tcggtcccca tgaccgagct tcgccgcagc gccgagcgcg
    14581 gccacgccaa ccacggctgg ctcgactcct accacagctt ctccttcgcc gattactacg
    14641 acccgaaaca catgggcttc ggcccgctgc gcgtgatcaa cgaggaccgc gtgcagcccg
    14701 gcaagggctt cggcacccac ggccaccgcg acatggagat catcagctat gtgctcgagg
    14761 gcgcgctcgg ccaccaggac tcgatgggca acggctcgac catcgttccc ggcgacgtgc
    14821 agcgcatgag cgccggcagc ggcgtgcgcc acagcgagta caaccatgag aagcagggcg
    14881 ccacccattt cctgcagatc tggatcgagc ccaacgtcac cggcattccg ccgtcctacg
    14941 agcagaagca cttcacgccc gcggacaagc gcggcaggct gcgcttgatc gcttctcccg
    15001 acggccgcga cggctcggtg accctccacc aggacgcgct ggtctgcgcg ggattgttcg
    15061 atggccgcga gaatgcgagc tacgagctga gaaacgagcg aggctacgtc cacgtcgcgc
    15121 gcgggcgcat caccgtgaac ggtcatgtgc ttgaggcagg cgacgcgctg aagaccgagg
    15181 acggcgtgat cgagctggag aacggcgagc aggccgaagt gctggtgttc gaccttccgg
    15241 agtagccgcc caggactgcc tcaggtctgc ttgagcaccc ggccgatcac ctcggcgagc
    15301 tggttcttgc tgtaaggctt ggtgatgtag ccgtcggcgc cgagcacgag tcctttgcgg
    15361 atgtcggcga gctctgcctt caccgtgagc agcacgatcg gcagcatggc gaattccggg
    15421 cgggcacgca gccggctgag gatgtcgaag ccgtttccgt cgggcagcat gacgtcgaga
    15481 agcagcaagt cggggcgggc ctgctcgcgc aggaagcgcg agagcgcctt ggcgcggtcg
    15541 acgctgcgca cctggtagcc cgccatctgc agccggagct ctccgagcgc catctggtcc
    15601 gggtcatcct ccaccagcaa gaccacggtt tttgcgggcg ctttggcgag cgccggcagg
    15661 ttggcgatgc ggtcgaccgc gagaaaggag cccgagcgca gcagcgtcgc gccggcgatg
    15721 accgtggtct tggcgaatcg caggctgtcc tcgtcgggca ggggcggcag ctgcggcacg
    15781 cggctgccgc tgaaggtgat gtccgccgcc ttgcccgcgg gccacctcgg ctcgatcatc
    15841 ttcaggtcct cgagctcctt cagccattct tcgatcagcc ggtcggggaa gcggcgcagc
    15901 cgcccgcgga tcacgtcgat atggccctcg agctccatca gggcgaggag gcggcggtac
    15961 tcgaggggca gcttcgccgc gccatccttt tcccgcaggg cgtcctttcc tgcgggggta
    16021 aggtagtaca ccagggccat gggcagttat tgtcggcgat taccgcgtcc ggggagcgtg
    16081 gagcttgccg ctcacccgcg taaaggttcc ttcaccggcg caaagatatg tgggacctcg
    16141 ctatccgcag acgcctacgt atccgcaggc ggtgcagtag tcgcagccgt ccttgcggat
    16201 gagcgtcggg ttcgcgcact cgggacacct ggcgccggcg atgaccggcg tgctcttcac
    16261 ggccggcgct tcgaggattc ccatctgctg cttggcgctg ccgacgtcgt cgaggatgcc
    16321 gagcatggcg tagcggtgca ggatcagccg cgccaggtac gacacggtcg agggaaaggt
    16381 ctgctgcttg cggctccccg ggacgaaggc catgaagtcg ccgagcggct cggggaactc
    16441 cagcagcttc ttcagcttca tcccgaccca cgcggggtcg agcacccgca tgtcgagcga
    16501 gaggatcttg cacagcccgt cgagggcgcg cgggtactcg ccggagagcc atacggaata
    16561 gggccgggtg atgccatccg gcaaggtaat ctcttttaga cccaacacaa aatcatcgcc
    16621 ggtgcgggga ttgagcacgt cgacggtcca ggacatggtg ccgtcggtgc cggtcttggg
    16681 ctccttcagc gcgaacatgg catcgaggac tggagacgag gcctcttctc ccgcaagctg
    16741 gaactcgtcg ccggccaagc ctgcttgcaa ctgctcgacg cgccagcgga cgatctgggc
    16801 gaaagcggag accacggagg gcatcagctt cttctcgccg cgcggcggga acggcatctc
    16861 gaagggctcg tcgccgccgg ctttggcgag cgtgtcgagc ttgagcttga gccacgcgct
    16921 gtcgtccgcg cgcatgtcca tcgacagcgt tttggcgagc gcgcccaggc cgcgcggctg
    16981 ctcgctgccg ttgacccaga cttcaaatgc gtggggtctg ccaccgctgt gcggcgcgga
    17041 tcctggcgcc gccgacccat tctcgacgtg gccgacgaac accgcgaagc ggtactgcgg
    17101 gtgctcgatc atgtaggtcc acgccgggtt gccgccggag aggtcggggc ggccgggcca
    17161 gcgcagcgag gcgagcaccg gcgccgggac cgatttcacc tggatgcgcc ggttgacgtc
    17221 gtcctgga
//
LOCUS       DATBSZ010000297        24094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_198683, whole genome shotgun sequence.
ACCESSION   DATBSZ010000297 DATBSZ010000000
VERSION     DATBSZ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 24094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24094
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198683"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /gene="typA"
                     /locus_tag="VIG70_19555"
     CDS             <1..1770
                     /gene="typA"
                     /locus_tag="VIG70_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY8252793.1"
                     /translation="HVDHGKTTLVDKLLAQSGTFAAHEHVQERVMDSNDLERERGITI
                     LAKNCAIRYRGTHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAVEGPMPQTRFVTR
                     KALALGLKPIVVVNKIDRPGARAEWVVNQTFDLFDKLGASEDQLDFPVVYASALLGYS
                     KSNLNDEGRDMQPLFEAILEHVPVRDEDPDAPLQLQICSLDYSSYVGKIGIGRIRRGR
                     IRAAQEVLLLPENIKAKVNQVLMFEGLERKPVDEAEAGDIVLINGIDEVGIGATLCDP
                     EHPEALPALKVDEPTLTMNFLVNASPLAGREGKYVTSRQIRERLERETKSNVALRVEY
                     PSDTDTFIVHGRGELHLTILLETMRREGYEIAVSRPRVIFKEIDGQRCEPYELLTVDV
                     EDANQGAVMEELGRRRGELQDMQPDGKGRVRLDYRITARGLIGFQGEFMTLTRGTGIM
                     SHVFDDYGPVKAEIDEHRNGVLVSQDDGEAVAYALWKLQERGRMFVSPGEPVYEGMII
                     GIHSRDNDLVVNPIKTKQLTNIRASGKDEAILLTPPIELTLEYAVEFIGDDELAEITP
                     KSIRLRKRFLKENERKRAERAAA"
     gene            complement(1767..2960)
                     /locus_tag="VIG70_19560"
     CDS             complement(1767..2960)
                     /locus_tag="VIG70_19560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8252794.1"
                     /translation="MLVIGAGAAGLAAADELTRAGRSVLLAEARDRVGGRCLTRRLPG
                     VPVPVELGAEFIHGRPQTTLSLLQRAGIPAVDSTRTQLIAFDGKLRPVNMFTQATRVA
                     RRPVKGRDISFRAFLARQRLPRLTRTLGTMMVQGFDAADPGKASAREIVDEWSELSSS
                     QPRPYGGYGPLLEGLARGLQVQLETVVRRVRWSRGSVEVDGTFRGEPWRAWAPRAVIT
                     LPIGVLPSLRIREKSDALTKLASGPVIRVAMAFRSAFWEKKHSGVAFFHSPQAPFPTF
                     WTPLPMHAPLLTAWAGGPKAARLTGSSQKILLRKALESVRSVLGNVEEPGAFLIHDWQ
                     ADPHARGGYSYVKVGGTGAREALAEPLEDTLYFAGEATDVEQSGTVGGALASGIRAAR
                     EILEN"
     gene            complement(2978..5686)
                     /locus_tag="VIG70_19565"
     CDS             complement(2978..5686)
                     /locus_tag="VIG70_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY8252795.1"
                     /translation="MDRSRGVGPFKYFVGISSLEEVATRKDRVCVLNILGGESSEVTP
                     VSHAYSGGNVVFGTSPGRQGQVLKTPIGEVPVYNSVREGLDAGHRFNVGVVYLPPSGV
                     RDGVAELVRVNPDLRKIVMVTEKVAVHDAREIRAMGQQSGIDIFGANCLGVADSWNRV
                     RIGGALGGDDPAESLVKGSVAIYSNSGNFTTTIAQYLAAAGWGTTTLVSSGKDVYIHF
                     AAPEFVFALANDRRSKAAVIYAEPGGYYEYDLNFTKPVVACVVGRWKARLTRAVGHAG
                     AMGGSGDDALAKERWFMQSFGVEKLFTPEDPVCSPRGAVVTNIAHIPLALTRVMKLNR
                     IEPDFAPRGSLALKPWFGASRGLALPPELDLPVVEAVAPYHEHIAQLNRQIGRTFPRQ
                     SMRDTSGASVMDAKTQITQVHGVSVLEAAKRPLESNLCHALLREPGGANDSALMNVAV
                     TAGVNLHGDVMLAAAQAAREAGNAPNSVLAAACSIHGPNRVAAVRKATDALIDLFANS
                     GLQDAGDEAFDFSKMSIGDQERPLLVGPKPDPKAEAMLDALAARKTRSVFVRYLRALG
                     GPPTRDAVLAAICATLAWGPLMRKRISRETVRGLPWHVGLWATLIGASVDATRHEPNR
                     FCGISNEELAASWSATELAYLTLIGERAKPEELFAFQVLTGLLISNAVGSISAQGCKG
                     AVSSDGPETPGRVQVNKAMVGFLTHTGYSHGGAGYEGIIFLVEQFRDSGLKDPASPAH
                     GIDLKAMARRFAAGYARQKTQLKESGAERMRAIAGLNHPVFRDKPVNQDPREAFIREL
                     FRGRGEYNVFHEYYHELARALFEAGVTRNVFCVNIDGVIGALLLKMLWQRYRGGGFSE
                     HDLEIAAFTIFLYGRMIGCAAEIDDHINRGRNMDTRAPASQCRYVA"
     gene            complement(5688..6962)
                     /locus_tag="VIG70_19570"
     CDS             complement(5688..6962)
                     /locus_tag="VIG70_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HEY8252796.1"
                     /translation="MQLTGMLHGSKLLQFVDFPAAKVLGPEASEDEISALIESHGSVF
                     VKPIFKGGVGKKGKSGLIGRAKDLKAALAEKERLYFVEHRVGNVVSKAQGVTFEGAVP
                     AEHEVYFAIGDSTRFRAPTITLTHMGGMAIEELDRKHVAEVPFEALTGLKAFVIANAL
                     TDIGAPREIISPLVQNLPKLWELFHHYGLTTLELNPIRMSPGRGGRLVPVACDFKCGF
                     DRDDPRRERLNLPASLFAGDYSDFEREINQLRTYQGQSDVFVINPKGTILAPTFGGGA
                     NSLVTQALGDDAIISSDFGGNPPYEKMHDVARICFKYWLAQSNVLFIIGGKSNNTDIF
                     ETLRAMADALREHFGAHGPTPLFVVIGRGGPNLVRGMGAMRDTMQALGLPYRFFGFDS
                     AMSEVVNYALAADRWMKSGGRAQVAKAMGLEN"
     gene            complement(6973..8472)
                     /locus_tag="VIG70_19575"
     CDS             complement(6973..8472)
                     /locus_tag="VIG70_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY8252797.1"
                     /translation="MEALNALLAGFGVALQPMNLLWGLIGVTLGTFVGVLPGVGPALT
                     VAMLLPLTVKLDPTGALIMFAGIYYGAMFGGSTTTILLNTPGESASIATALEGNRMAK
                     AGRAGPALATSAVGSFVAGTIATVLLTLLAPLVVDLALKFGPAEYFALMVFAFITVAA
                     VLGNSTARGLTSLFLGLFFGLIGIDSQTGQPRFAFGVPELLDGVDVVVLAVGLFAVGE
                     ALYVAAYQSRLAETVEKVRGSLLMSASDWKRSWPAWLRATAIGFPFGTIPAGGSEIPT
                     FLSYATEKKLTKHPEEFGKGAIEGVAGPEAANNAAATGVLVPLLTLGIPTSATAAILL
                     GAFQNYGLQPGPLLFESQKDLVWGLIASLYVGNIILLILNLPLIGIWVKVLEIPKDLL
                     YAGILVFATLGAYSLHQSWVDLATLYVFGLVGFAMRRWDIPVGPAVIGLILGPLAETQ
                     FRRALSISQGDASVFFTQPISATFLAITAALIVVPFIVRHWGRLRRKPA"
     gene            complement(8486..8968)
                     /locus_tag="VIG70_19580"
     CDS             complement(8486..8968)
                     /locus_tag="VIG70_19580"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY8252798.1"
                     /translation="MRRSPAEIALSLGVLALGVGVAAVTATLPSEGGYSGIGPNFIPA
                     VVAGGIIVLGVWLGVEAFTGGWRDVAEHDAVFEASPFLWISAGLFAHMALIGWAGFVI
                     AGSVLFVCVARGFGSRRLIRDLSIGIVLALGVYLFFVKLLNVNLPAGWLAPLLGGAGI
                     "
     gene            complement(8976..9929)
                     /locus_tag="VIG70_19585"
     CDS             complement(8976..9929)
                     /locus_tag="VIG70_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8252799.1"
                     /translation="MPRRWFMRFLIALAVALVAGTAAAQPSLKMMIPANPGGGWDQTG
                     RNLAAAMQSAKLVSGVQYDNKGGAAGTIGLAQFVNSAKGDPNAVMIGGMVMVGGIILN
                     KSPVNLSQVTPIARLTSEWEVIVVPANSPHKTMADLVKALKDNPGKVSWGGGSAGGTD
                     HILVGLIAKDVGVDPAKINYVPFKGGGEAIAAILGGHVTAGVSGIGEFAEQIKAGKMR
                     ALAQSAPKKVEGIASLKEQKINVELGNWRGIFGAPGITPQQRDALVKLVRSATETPAW
                     KGTLEKLGWEPWFLGGEEYKKFLEEDTKRVAAIIESLGLKK"
     gene            9993..10913
                     /locus_tag="VIG70_19590"
     CDS             9993..10913
                     /locus_tag="VIG70_19590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8252800.1"
                     /translation="MIRIENLVKSFGAKRAVDGVSFNVERGEVLGFLGPNGAGKSTTM
                     RMITGFMPPTAGKVTVGGHNVAESPLEAKRLIGYLPENAASYPDMTVKGFLEFVAELR
                     GLKGSKRKQAVNRVVELCFLDSVLRQSIDTLSKGYKHRTCLAQALIHDPEVLIMDEPT
                     DGLDPNQKHEVRNLIRTLGKNKAIVFSTHILEEVDAACTRAIIIDRGKIVANGTPEEL
                     RDMSNLAGAVTLSAHGASAEKLSALGRVEDLGGAFRIYPNDKSQSAELARGVVELVAR
                     EGWKVEGMFSERGELDEVFRRITLPDTVKR"
     gene            10910..11644
                     /locus_tag="VIG70_19595"
     CDS             10910..11644
                     /locus_tag="VIG70_19595"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8252801.1"
                     /translation="MRIIWTIAKRELSAYFTSPVAYVFLVIFLLLTGFFTFTAGNFFE
                     RGEASLAAFFGWHPWLYLVLVPAVGMRVWAEERRSGTMELLLTMPVATWQAIVAKFLA
                     SWVFLALALALTFPTILTVNVLGDPDNGIIAAGYVGSLLLAGAYLAISCMTSAMTRNQ
                     VVAFILSVVVCLFLILAGFNPVTDLLTRWASPQVVDTVAAFSVVTHFDAFQRGVIDGR
                     DLMFFLSVIGFALFATGVIIRGHRSG"
     gene            11641..13476
                     /locus_tag="VIG70_19600"
     CDS             11641..13476
                     /locus_tag="VIG70_19600"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEY8252802.1"
                     /translation="MRKYEHLIYSAVGLIALFLLLVAVNFLVSRVPARLDLTQGNLYT
                     LSPGTKKVLRGLQAPVKVKLYVSQGEAVPVQLRGFAQRVEDLVREFKSVAGSNLVVER
                     YNPRPDSEEEDAAQLDGIEPQQLFSGEQFYLGVAVNQLDRKQSIPAISPQRERLLEYD
                     LVRAIARVGATERPKIGLMAGLPVLGERFNPFTRQSSEPWVLANELKREFEVKEVPLT
                     AKEIDKDLNVLLLIHPRDVPPSTEYALDQFVMRGGKLIAFVDPYAYFDQGPTMPGMPP
                     MPSSSTLPTLFKAWGLSMEPGKVIADVVFGSGGGARYTPTVLSLNRTAFSRDDIVTGQ
                     IETLLYAFGGAFELKLPENLKATELVKSSPNSMLVDAMNATKSGDEATRAFKPSGNAM
                     ALALRLTGKFKTAFPDGLKEDEKKPAAQGTPALRESPENSVIVVADVDMLADGAAVDV
                     QDVFGRKVVVPSNGNLAFALGMVEQFAAGDELISLRSRAASFRPLTVVRELEAEAQKE
                     YFGRIQALEEEKQKTQARLQELQKAQGGAAKGAQLLSAEQQAELERFKKTYIETNLAL
                     KELRKNLRQDAEALVFWTKVANIAFMPLLVALLGLLIAFLRKRAA"
     gene            13473..14498
                     /locus_tag="VIG70_19605"
     CDS             13473..14498
                     /locus_tag="VIG70_19605"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEY8252803.1"
                     /translation="MNARVAAILVVLLVVLGGGALLYQQQERTQRPENVATLGRSLFK
                     DLKVADIAVIRIVEPKGALTLQRKDDRWVIAERGEFPADLSRVREFVLKVIGLKVGQS
                     EPLGEKDRARLNLDASGTKVEFKGADGKDLGVFVVGRKYFKREVENPEKANPDGRFVG
                     LPQEAGTAYLVGDPLTQATVKTAEWIDRSSFQVEKVKTLQVRYPGGEGWRIDRRADNA
                     EWKLANAKPGEKLDISRANSASYSLSLLELADVAPKGDADTGLDKPTTIDATTLEGAA
                     YAIKVGKLAGENYYVTFTASGHKPDAKDAERVKLLSQHVLLIQKSKLEDTLKKRSELL
                     EKKANKK"
     gene            14614..15042
                     /locus_tag="VIG70_19610"
     CDS             14614..15042
                     /locus_tag="VIG70_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252804.1"
                     /translation="MKQLAAFAAFVCVATPSAAFDTLSQQSPGVMFFFSIPIDAPKAK
                     HETFSAGFAIQGKREYETVKIDSRMLNNFIGGGIEAKWIIAGVVAAGTAVAVASKDKS
                     TTTSQQQQQQAAQQQAQQQAQQTGGGTSPGTPCPVKPNCP"
     gene            15092..15532
                     /locus_tag="VIG70_19615"
     CDS             15092..15532
                     /locus_tag="VIG70_19615"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEY8252805.1"
                     /translation="MAFDPHVPEIAGVIQLAIAPVFMLTAVGTVIGALNIRLGRAVDR
                     RRELEERLSAMSQAELPSAQEELGTIARRIRFVYLSIVFAVISALFIALLIAGAFVGA
                     FVRADLSFTIAGMFICAMLALIVCLLLFLREIFLAVSTPRHVPR"
     gene            15563..17614
                     /gene="fusA"
                     /locus_tag="VIG70_19620"
     CDS             15563..17614
                     /gene="fusA"
                     /locus_tag="VIG70_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY8252806.1"
                     /translation="MAHSIKRIRTMALVGQSGAGKTTLAEALLAKAGTIPAAGSVERG
                     TTVCDYTPLEKQLQHSLKLAVASFDFQEHRIHLLDTPGYPDFLGHALPAFAAVETAAI
                     VINAQNGLEMMTGRFVQWAAKRGLDRLIIVNKIDADNVDLEDLLERIQDAFGKECLPL
                     NLPAANRTKVSDCFFAPSGESDFSSVDEAHRKLVDQVVEVDEKLMEAYLEKGEVSPDE
                     LHEPLERALREGHLIPVVFTSAKTGAGIGELLDVIVNLLPNPTEGNPPSYVVTKAGNG
                     QGTDLTPIPDPGKHALAHVFKIEIDPYIGRLAVFRVHQGRLTPGGQLFIGEGRKPIKA
                     GHLYLLRGKTQVEVHEAIPGDICAIAKIDEIQFDHILHDSAEDAHVHARPLELPTPVF
                     GLAVQPKKRGDEQKVSDVLHKIIAEDACFRLEHNSQANEMVMRGLGEMHLRTILEKMS
                     TQYKLELDTRPPSIAYRETIAARAEGHSRHKKQTGGAGQFGEVYLKIEPLPRGKGFEF
                     VDSVKGGAIPNQFIPSVEKGVRGVLDHGFLAGYPLQDVRVTVYDGKHHPVDSKDVAFV
                     SAGRKAFLDALAKARPIVLEPIVNVEIVAPEERMGDIAGELSGHRGQIKGSDAPRPGL
                     VQIKATAPLSELEHFPARLKSLTAGHGTYNLEFSHYEPAPPQLQQKLVAAHKPAVGAE
                     D"
     gene            17629..18399
                     /locus_tag="VIG70_19625"
     CDS             17629..18399
                     /locus_tag="VIG70_19625"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8252807.1"
                     /translation="MRQGRGAPPLVFVHGFLCSHEDWKFQVRELQRNHEVVACDLRGH
                     GETPGRPQECSIEHFGGDVAALVNNLELPNPILVGHSMGCRVVLEANRIAPERIAGIV
                     LVDGSRFSAGDPDAAEAAARSQIEQVGFAAWAEELFRQMFLTSTPASERLVARAVRQG
                     AEIGAALWPRSVRWDAGALEAALAAVRAPVLAIQTTTRDPQSGKRGPLKAGQTTPWLD
                     LLRTRIPTCRVEILPGLGHFPQLEAPERVNRLIEEFCR"
     gene            complement(18368..19354)
                     /locus_tag="VIG70_19630"
     CDS             complement(18368..19354)
                     /locus_tag="VIG70_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8252808.1"
                     /translation="MAFKVGLIGLGMAVTPHAKSLQDLRSRVEVAYAYSPSAARRKSF
                     AERFPFPQCDRLETILDDRSVNAVLVLTPPNTHLEIVERCAAAGKHVLLEKPLEISTA
                     RAERLVQSCKNMKLGMVLQHRFRPAAEKLREMLPLLGKLVSASAAIPNWRPQSYYDQP
                     GRGTRARDGGGVLLTQGIHTLDLFLSYTGEPAEVKSFVATTPVHKMETEDLAAAAVRF
                     KNGALGTVHATTAAYPGFPERIELIGTQGTALLEGTSLKMSFSDAQSFEMKTESGGGG
                     TGADPMAFPHDWHRGVLADFLDSIEQNREPRVSGTEALKVHRFIDRILRSGG"
     gene            complement(19344..20159)
                     /locus_tag="VIG70_19635"
     CDS             complement(19344..20159)
                     /locus_tag="VIG70_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8252809.1"
                     /translation="MKISVPKGACDTHMHFYDQALPAAPGTFLPGHFPVEDYRALQKK
                     LGLERVIVVQANAYKDDNSVIVDSIRKLGESAKGVAVVTPGVADAELERLTKAGICAV
                     RIMTLHGGMLGFDLMDPVMARVHPFGWHANIQLDGRELPKYEAQIKRLPGKFVIDHTG
                     KFLEPVSPESHEFRSLLNLVDTGRCWVKLSAPYETSKTGAPKYEDVSRLAKALVKKAP
                     ERMLWASNWPHPSARKPAPPDDAALLDLLLNWAPIENDRKKILVDNPAELYGF"
     gene            complement(20156..20980)
                     /locus_tag="VIG70_19640"
     CDS             complement(20156..20980)
                     /locus_tag="VIG70_19640"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8252810.1"
                     /translation="MKTVLITGATGDVGTHLARELAAKYKLRLSDRRPLKAKNFIKAD
                     ISKMADALRITRGVDAVVHLGGYSVEGPWEAILQANIIGCYNVFEAARRNGVKRIVFP
                     TSNHATGFYRRDQTIDHRVYPKPDGRYGVSKVFGEALGSLYADKYGLEFLMVRIGNVN
                     PAPLDKRRLSLWISPRDIAQLVSIGIDHPGIKFEIVYGVSGNKRNWYDNSNATRLGYR
                     PQDDSEPFAEEILRKEKPGSDAIAEAHQGGIFCTAEEVPNPAAPPKKPKAKKGKKK"
     gene            complement(20977..21786)
                     /locus_tag="VIG70_19645"
     CDS             complement(20977..21786)
                     /locus_tag="VIG70_19645"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8252811.1"
                     /translation="MRRAGRLRQEGGKGAGAEIYYEAVGEGPAVIFAHGLGGNHLSWW
                     QQIAHFAPRYTCVAFAHRGFPPSSPVPGSSAPDSYADDLAALIRELDLKDVALVAQSM
                     GGWTCLEYALREPRKVRALVMASTSGALDFSHLENAEISEWIDRSPAGLAELQSRGIH
                     PAGGERMAREQPALAQLYWQISEMAPASFREDVRVRIRDLRTQTPSLLAKLSMPVQFI
                     TGDEDWVFPPAAGPALASIAPKGSAVRVPAAGHSVYFERAAQFNDIIGRVL"
     gene            complement(21783..22712)
                     /locus_tag="VIG70_19650"
     CDS             complement(21783..22712)
                     /locus_tag="VIG70_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8252812.1"
                     /translation="MANYYGERALAGLRAVGDVKLNQKSDSLEGEALIAAAEGCDLIV
                     SYRQSPGPAAVFERLPRLIAFLRCAIDIRNVDVAAASKAGVLVTQASAGFITSVTEMV
                     LGFLVDLSRGITRSATLYHQNRVPKATMGRELKGSTLGVIGYGAIGKEVTRMGRALGM
                     RVLVHDPYQQVDQVPLDTLLRESDYVVPLAVATAETENLMNAEAFFKMKKGACLINVS
                     RGNLVDEAALEAALDSGRLAGCAMDVGRASDQMPTPRLAARPDVIATPHTAGLTMPAI
                     EHQSLETVAQAAEIVKGRVPKGAVNAEHWKRKP"
     gene            complement(22742..23473)
                     /locus_tag="VIG70_19655"
     CDS             complement(22742..23473)
                     /locus_tag="VIG70_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY8252813.1"
                     /translation="MRIRPVAAERKLSRRLFEQLAEQIRSGRFAPGARLPTEQALTQA
                     AGVSRTVVREAVAALRAEGLVVTRQGVGAFVSAEPQRAPFRIEPERMQTLDDILAVME
                     LRLGVEIESAGLAADRASKAQVRTISNALDAIEEASVAGRSAVDEDLEFHRAIAEATG
                     NPEFPRFLQFIGRHLIPRRTVSGLPERMGGERAYLALIQEEHRRIYQAIESADAKGAR
                     EAMRRHLTRSLERYRKLAAEQKKAA"
     gene            23547..>24094
                     /gene="dctP"
                     /locus_tag="VIG70_19660"
     CDS             23547..>24094
                     /gene="dctP"
                     /locus_tag="VIG70_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY8252814.1"
                     /translation="MFRRHFIGAASVAAVSVSLLSALPVQAQQIIKATDVHPLGYPTV
                     EALVRMGNKLEKATNGKYKMQMFPSMQLGGEKEMIEQAQVGALQIARISVGPMGPIVD
                     ELNVFNMPFVFKDEAQMRKVIDGPIGEEMLTKLSNSSARLIALGWMDAGTRNVYSDKP
                     VTKPADLKGMKIRMMGNPLFVET"
BASE COUNT         4091 a         8136 c         7974 g         3893 t
ORIGIN      
        1 cacgtcgacc acggcaagac gaccctggtc gacaagctgc tcgcgcagtc gggcaccttc
       61 gccgcgcacg agcacgtgca ggagcgggtc atggactcga acgacctcga gcgcgagcgc
      121 ggcatcacca tcctcgccaa gaactgcgcc atccgctacc gcggcacgca tatcaacatc
      181 gtcgacaccc ccggccacgc cgacttcggc ggcgaggtgg agcgcgtgct gtcgatggtc
      241 gacggggtgc tgctgctggt cgacgcggtc gaggggccga tgccgcagac gcgcttcgtc
      301 acccgcaagg cgctcgccct gggactgaag cccatcgtcg tggtgaacaa gatcgaccgc
      361 cccggcgcac gcgccgagtg ggtggtgaac cagaccttcg acctcttcga caagctcggc
      421 gccagcgagg accagctcga ctttcccgtg gtctacgcct ctgccctcct gggttactcg
      481 aaaagcaacc tgaacgacga gggtcgcgac atgcaacccc tgttcgaggc gatcctcgag
      541 cacgtgccgg tgcgcgacga ggatcccgac gcgccgctgc agctccagat ctgctcgctc
      601 gactactcga gctacgtcgg caagatcggg attggccgca tccgccgcgg gcgtatccgc
      661 gcggcacaag aggtcttgct ccttccggag aacatcaaag ccaaggtcaa tcaggtcctg
      721 atgttcgaag gtctggagcg caagccggtg gacgaagcgg aggccggcga cattgtgctg
      781 atcaacggca tcgacgaggt cggcatcggc gccaccctct gcgacccgga gcatcccgag
      841 gcgctgcccg cgctcaaggt cgacgagccg acgctgacca tgaacttcct ggtcaacgcc
      901 tcgccgctcg ccgggcgcga gggcaaatac gtgaccagcc gccagatccg cgagcgcctg
      961 gagcgcgaga ccaagagcaa cgtcgcgctg cgcgtcgagt atcccagcga caccgacacg
     1021 ttcatcgtgc acggccgcgg cgagctgcac ctcaccatcc tgctcgagac catgcggcgc
     1081 gagggctacg aaatcgccgt ctcgcgcccg cgcgtgatct tcaaggagat cgacggccag
     1141 cgctgcgagc cctacgagct gctcaccgtc gacgtagagg atgcgaatca gggcgcggtc
     1201 atggaggagc tcggccgccg gcgcggcgag ctgcaggaca tgcagcccga cggcaagggc
     1261 cgcgtgcgcc tcgactaccg catcaccgcg cgcggcctga tcggcttcca gggcgagttc
     1321 atgaccctca cgcgcggcac cggcatcatg agccatgtgt tcgacgacta cggcccggtt
     1381 aaggccgaga tcgacgagca ccgcaacggc gtgctcgtgt cgcaggacga cggcgaggcg
     1441 gtggcctacg cgctgtggaa gctgcaggag cgcggacgga tgttcgtcag ccccggcgag
     1501 ccggtgtacg agggcatgat catcggcatc cacagccgcg acaacgacct ggtcgtcaat
     1561 ccgatcaaga ccaagcagct caccaacatc cgcgcctcgg gcaaggacga agccatcctg
     1621 ctcaccccgc cgatcgagct cacgctcgag tacgcggtgg agttcatcgg cgacgacgag
     1681 ctcgccgaga tcacgcccaa gtcgatccgc ctgcgcaagc gcttcctcaa ggaaaacgaa
     1741 cgcaagcgcg ccgagcgcgc agcagcctag ttttcgagaa tctcgcgcgc ggcacgaatg
     1801 ccgcttgcaa gcgcgccgcc caccgtcccg gactgctcca cgtccgtcgc ttcgcccgcg
     1861 aaatagagcg tgtcctccag cggctcggcc agcgcctcgc gcgcgccggt gccgccgacc
     1921 ttcacgtagc tgtagccgcc gcgcgcatgc ggatcggcct gccagtcgtg gatcaggaac
     1981 gcgcctggct cctccacatt ccctagtacg ctccgcacgc tttcgagcgc ctttctcagc
     2041 aggatcttct gtgaagaacc cgtgagccga gccgccttcg gccctcccgc ccacgccgtc
     2101 agcagcggcg cgtgcatcgg cagcggcgtc cagaacgtcg ggaacggcgc ctgcggcgaa
     2161 tggaagaacg caaccccgga atgttttttt tcccagaacg cggagcggaa tgccatggcg
     2221 acgcggatca ccgggcctga cgcgagcttg gtaagcgcgt cgcttttttc tcggattcga
     2281 agtgaaggca agactccgat cgggagcgtg attactgcgc gcggcgccca ggcgcgccag
     2341 ggctcgccgc gaaacgttcc gtcgacctcg accgagccgc gcgaccagcg cacgcgccgt
     2401 accacggtct cgagctgcac ctggagtcct ctcgccagac cttcgagtag cgggccgtag
     2461 ccgccgtacg gacgcggctg tgaggaactg agctcggacc actcgtcgac gatctcgcgg
     2521 gcgctggcct tccccggatc ggccgcgtcg aagccctgga ccatcatcgt gccgagggtg
     2581 cgtgtaaggc gcggcagccg ctggcgcgca aggaacgcgc ggaaggaaat gtctctgccc
     2641 ttcaccggcc tccgcgcaac gcgcgtcgcc tgcgtgaaca tattgaccgg ccgcagcttg
     2701 ccgtcgaatg cgatgagctg cgtgcgcgtg gagtcgacgg ccgggatacc cgccctttgc
     2761 aggagagaaa gcgtggtttg cgggcggccg tggatgaact cggcgccgag ctcgaccggc
     2821 accggaactc ccggcaggcg gcgcgtgagg cagcggccgc ccaccctatc tcttgcctct
     2881 gcaagcaaga cggagcgacc ggcgcgcgtg agctcgtcag ccgcagcgag gcccgcggcg
     2941 cccgcgccga tgacgagcac gtcgcaggag gttttgctca ggccacgtag cggcactgcg
     3001 acgccggcgc gcgcgtgtcc atgttgcggc cgcgattgat gtggtcgtcg atctcggcgg
     3061 cgcagccgat catgcggccg tagaggaaaa tggtgaacgc ggcgatctcg aggtcgtgct
     3121 cggagaagcc gccgccgcgg tagcgctgcc agagcatctt caagagcagc gcgccgatga
     3181 cgccgtcgat gttgacgcag aagacgttgc gcgtgacccc ggcttcgaac agcgcgcgcg
     3241 cgagctcgtg gtagtactcg tgaaacacgt tgtattcccc gcggccgcga aacagctcgc
     3301 ggatgaacgc ttcgcgcgga tcctggttca ccggcttgtc ccggaacacc gggtggttga
     3361 gcccggcgat cgcgcgcatg cgctcggccc ccgattcctt gagctgcgtc ttctgccgcg
     3421 cgtacccggc ggcaaagcgc ctggccatcg ccttgaggtc gatgccgtgc gccgggctcg
     3481 ccgggtcctt cagccccgag tcgcggaact gctcgacgag gaagatgatg ccctcgtagc
     3541 cggcgccgcc gtgcgaatag ccggtgtgcg tgagaaagcc gaccatcgcc ttgttgacct
     3601 gcacgcggcc cggcgtttcc ggcccgtcgg aggacacggc gcccttgcag ccctgcgccg
     3661 agatgctgcc gaccgcgttc gagatcagga ggccggtgag cacctggaag gcgaacagct
     3721 cttcgggctt cgcgcgctcg ccgatcagcg tgaggtaggc gagctcggtt gccgaccatg
     3781 acgcagccag ttcctcgttg gaaatcccgc agaaccggtt cggttcgtgc cgcgtcgcgt
     3841 ccaccgaagc gccgatcagg gtcgcccaca accccacgtg ccagggcagg ccgcgcaccg
     3901 tctcgcgcga gatccgcttg cgcatcaacg gcccccaggc gagggtggcg cagatcgcgg
     3961 cgagcaccgc gtcgcgcgtc ggcggaccgc ccagcgcgcg cagataacgc acgaaaaccg
     4021 atcttgtttt gcgtgcagca agagcgtcaa gcatcgcttc tgccttcggg tcgggctttg
     4081 ggccgaccag cagcggcctt tcctggtcgc cgatgctcat cttgctgaag tcaaatgcct
     4141 cgtcgcccgc gtcctggagc ccggaatttg cgaacaagtc gatcagcgcg tcggtcgcct
     4201 tgcgcaccgc tgcgacgcgg ttcggcccat ggatgctgca cgccgcggcg agcaccgagt
     4261 tgggtgcgtt gccggcttcg cgcgccgcct gcgctgccgc gagcatgacg tcgccgtgca
     4321 gattgacgcc ggcggtgacg gcgacgttca tcagcgcgct gtcgttggcg cctccgggct
     4381 cgcgcagcag cgcgtggcac agattggact cgagcggccg ctttgccgcc tccagcaccg
     4441 atacgccgtg cacctgcgtg atctgggtct tggcgtccat caccgaagcg cccgaagtgt
     4501 ccctcatcga ctggcgcgga aacgtgcggc cgatctggcg gttgagctgc gcgatgtgct
     4561 cgtggtaggg cgccactgcc tcgaccacgg gaagatcgag ctcgggcgga agcgcgaggc
     4621 cgcggctggc gccgaaccag ggcttgagcg ccaggctgcc gcgcggcgcg aaatccggct
     4681 cgatgcggtt cagcttcatc acgcgggtga gcgcgagcgg gatatgcgcg atgttggtca
     4741 cgaccgcgcc gcgcggcgag cacaccgggt cctccggcgt gaagagcttc tcgacgccga
     4801 agctctgcat gaaccagcgt tccttggcga gggcgtcgtc tcccgagccg cccatggcgc
     4861 ccgcatggcc gacggcgcgc gtcaggcgcg ctttccagcg ccccaccacg caagccacca
     4921 ccggcttggt gaagttgagg tcgtattcgt agtagccgcc cggctcggcg tagatcactg
     4981 ccgccttgct gcgccggtcg ttggcgagcg cgaacacgaa ttccggcgcg gcgaaatgga
     5041 tgtacacgtc cttgccgctc gagaccagcg tggtggttcc ccagccggcg gctgcgaggt
     5101 attgcgcgat ggtggtggtg aagttgcctg aattcgagta gatcgccacc gagcccttga
     5161 cgagcgactc ggccggatcg tcgccgccca gcgcgccgcc gatgcgcacc cggttccacg
     5221 agtcggcgac gccgaggcag ttggcgccga agatgtcgat gccgctctgc tgccccatgg
     5281 cgcggatctc gcgcgcgtcg tgcaccgcca ccttctcggt gaccatcacg atcttgcgca
     5341 ggtcggggtt gacgcgcacc agctccgcga cgccgtcgcg cacccccgaa ggcggcaggt
     5401 agaccacgcc gacgttgaag cggtgcccgg cgtcgaggcc ttcgcgcacg ctgttgtaga
     5461 ccgggacttc gccgatcggc gtcttcagca cctgcccctg gcgccccggc gaggtgccga
     5521 acaccacgtt gccgccggaa taggcgtgac tgaccggcgt gacctcgctc gattcgccgc
     5581 cgaggatgtt gagcacgcag acgcgatcct tgcgcgtcgc cacttcttcc agcgagctga
     5641 taccgacgaa atacttgaaa ggccctacgc cgcggctgcg atccatatca gttctccaga
     5701 cccattgcct tggcgacttg ggcgcgaccc ccgctcttca tccagcggtc ggcggcgagc
     5761 gcatagttga ccacctcgct catcgcggag tcgaagccga agaagcggta aggcaggccg
     5821 agcgcctgca tcgtgtcgcg catggcgccc atgccgcgca ccaggttcgg cccgccacgc
     5881 ccgatgacga cgaacagcgg cgttggcccg tgcgcgccga agtgctcgcg cagcgcgtcg
     5941 gccatggcgc gcagcgtctc gaagatgtcg gtgttgttgg acttgccgcc gatgatgaac
     6001 agcacgttcg actgcgccag ccagtacttg aagcagatgc gcgccacgtc gtgcatcttc
     6061 tcgtagggcg ggttgccgcc gaagtccgac gagatgatgg cgtcgtcgcc gagcgcctgg
     6121 gtgacgagcg agttggcgcc gccgccgaag gtcggagcga ggatcgtgcc cttcgggttg
     6181 atcacgaaca cgtcgctctg gccctggtag gtgcgcagct ggttgatctc gcgctcgaaa
     6241 tcggagtagt cgcccgcgaa caggctcgcc ggcaggttga gccgctcgcg ccgcgggtcg
     6301 tcgcggtcga agccgcactt gaaatcgcag gccaccggca cgaggcgccc gccgcggccc
     6361 gggctcatgc ggatcggatt caactcgagc gtggtcaggc cgtagtggtg gaacagctcc
     6421 cacagcttcg gcaggttctg caccagcggc gagatgatct cgcgcggcgc gccgatgtcg
     6481 gtgagggcgt tggcgatgac gaaggccttc aggcccgtga gcgcctcgaa cggcacttcg
     6541 gcgacgtgtt tcctgtcgag ctcctcgatc gccatgccgc ccatgtgcgt gagcgtgatg
     6601 gtcggggcgc gaaagcgcgt cgaatcgccg atggcgaaat acacctcgtg ctcggccggc
     6661 accgcgccct cgaaggtcac gccctgcgcc ttggaaacga cgttcccgac ccggtgctcg
     6721 acaaagtaca ggcgctcctt ttcggcaagc gcggccttga gatccttcgc gcgtccgatc
     6781 aggccgctct tgcctttctt gcccaccccg cccttgaaga tcggcttgac gaacaccgag
     6841 ccgtggctct cgatgagagc gctgatctcg tcctcggagg cctcggggcc gagcactttc
     6901 gccgcaggga agtcgacgaa ctgcagcagc ttggagccgt gcagcattcc ggtgagttgc
     6961 atgggaccct ccttacgcgg gcttgcggcg caggcgcccc cagtggcgca cgatgaacgg
     7021 cacgacgatc aaggcggcgg tgatggccag gaatgtcgcc gagatcggtt gcgtgaagaa
     7081 cacgctggcg tcgccctggc tgatcgagag tgcgcggcgg aactgcgtct cggcgagcgg
     7141 cccgaggatg aggccgatca ccgcaggtcc gaccgggatg tcccagcgcc gcatggcgaa
     7201 gccgaccagt ccgaagacgt acagcgtggc gagatcgacc caggactggt gcaggctgta
     7261 cgcgccgagc gtcgcgaata ccaggatgcc ggcgtacagc aggtccttgg ggatctcgag
     7321 caccttcacc cagataccga tcagcggcag gttgaggatc agcaggatga tgttgccgac
     7381 gtagaggctg gcgatcaggc cccagaccag gtctttctgc gattcgaaca gcaacggtcc
     7441 gggctgcagc ccgtagttct ggaacgcgcc gagcaggatc gcggccgtcg cagaggtggg
     7501 tattcccaag gtcagcaagg gaacgagaac accggtcgcc gccgcgttgt tggccgcttc
     7561 cgggcccgcc acgccctcga tcgcgccctt gccgaactcc tcggggtgct tggtcagctt
     7621 tttctcggtc gcatacgaga gaaaggtcgg gatctcggag ccgccggcgg gaatcgtgcc
     7681 gaagggaaac ccgatcgcgg tcgcgcgcag ccatgccggc caggagcgct tccagtcgga
     7741 agcggacatg aggagcgatc cccggacctt ttcgacggtt tccgccagcc ggctctggta
     7801 cgccgcgacg tagagcgcct caccgacggc aaacaggcct acggccagca ccacgacgtc
     7861 cacgccgtcg aggagttccg ggacgccgaa ggcgaagcgc ggctggccgg tctggctgtc
     7921 gatgccgatc aggccgaaga acaggccgag gaacaggctc gtcaggccgc gcgcggtgga
     7981 gttgccgagc accgcggcca cggtgatgaa ggcgaacacc atgagcgcga agtactcggc
     8041 cggcccgaac ttgagcgcga gatccacgac cagcggggcg agcagggtga gcaggacggt
     8101 cgcgatggta ccggcgacga acgagccgac tgcgctcgtc gcgagcgcgg ggccggcgcg
     8161 tcccgctttc gccatgcggt tgccctcgag cgcggtggcg atcgatgccg attcgcccgg
     8221 cgtgttcagc aggatagtgg tggtcgagcc tccgaacatc gcgccgtagt agatcccggc
     8281 gaacataatg agcgcgccgg tcgggtcgag cttgaccgtg agcggcagca gcatcgccac
     8341 cgtaagcgcc ggcccgaccc ccggcagcac gccgacgaac gtgccgagcg tcacgccgat
     8401 caggccccac agcaggttca tcggctgcag cgcgacgccg aaaccggcga gcagggcgtt
     8461 cagcgcttcc attgcctcat tttcgttata tgccggcgcc gccgaggagc ggcgcgagcc
     8521 agcctgcagg caggttcacg ttgagcagct tgacgaagaa cagatacacc ccgagcgcga
     8581 gcacgatgcc gatcgagaga tcccggatca ggcgccggct gccgaagccg cgcgccacgc
     8641 atacgaaaag cacgctgccg gcgatgacga aacccgccca gccgatgagc gccatgtgcg
     8701 cgaaaaggcc cgcgctgatc cagaggaagg gactcgcctc gaacaccgcg tcgtgctcgg
     8761 cgacatcgcg ccagccgccg gtgaacgcct cgacgccgag ccacacgccg agcacgatga
     8821 ttccgccggc gaccacggca gggatgaagt tcggcccgat gcccgaatag ccgccctcgg
     8881 agggcagggt cgcggtgacc gcggccacgc cgacgccgag cgcgagcacg cccagcgaga
     8941 gcgcgatctc ggccggcgac cggcgcatca caccgctatt tcttcaagcc cagcgattcg
     9001 atgatggccg caacgcgctt ggtgtcctct tcgagaaatt tcttgtactc ctcgccgccg
     9061 aggaaccacg gctcccagcc gagcttttcg agcgtccctt tccaggccgg cgtctcggtc
     9121 gcggaccgga ccagcttcac cagcgcgtcg cgctgctgcg gggtgatgcc cggcgcgccg
     9181 aagatgccgc gccagttgcc gagctcgacg ttgatcttct gctccttcag cgaggcaatg
     9241 ccctcgacct tcttcggcgc cgactgcgcc agcgcgcgca tcttgccggc cttgatctgc
     9301 tcggcgaatt cgccgatgcc cgagacgccg gcggtgacgt gcccgccgag gatcgcggcg
     9361 atcgcctcgc cgccgccctt gaacggcacg tagttgatct tcgccgggtc gacgccgacg
     9421 tccttcgcga tcaagccgac caggatgtgg tcggtgccgc ccgcggagcc gccgccccag
     9481 gacaccttgc ccgggttgtc cttgagcgcc ttgaccagat cggccatcgt cttgtgcggc
     9541 gagttcgccg gcaccacgat cacctcccac tcgctcgtca agcgcgcgat cggcgtgacc
     9601 tgcgagaggt tcaccggcga cttgttgagg atgatgccgc cgaccatcac catgccgccg
     9661 atcatcacgg cgttgggatc gcccttggcc gagttcacga actgggcgag gccgatggtg
     9721 ccggccgcgc cgcccttgtt atcgtactgc acgccggaaa ccagcttcgc gctctgcatc
     9781 gccgcggcga ggttgcgccc ggtctggtcc cagccgccgc ccgggttggc ggggatcatc
     9841 atcttcaggc tcggctgcgc ggcggctgtg cccgcgacaa gcgccacggc tagcgcgata
     9901 agaaaacgca tgaaccacct cctcggtatg ggtttcgatc gacaatttaa gcgataatcc
     9961 gcccccttga aagagcctct ccccgcaatt gcatgatccg catcgaaaat ctggtcaaga
    10021 gcttcggcgc caagcgcgcc gtcgacggcg tttcgttcaa cgtcgagcgc ggcgaggtgc
    10081 tcggcttcct cggcccgaac ggcgccggca agtcgaccac catgcgcatg atcaccggct
    10141 tcatgccgcc gaccgctggc aaggtcacgg tcggcgggca caacgtggcc gagtctccgc
    10201 tcgaggcgaa gcggctgatc ggctacctgc cggagaacgc cgcctcctac cccgacatga
    10261 cggtgaaggg gtttctcgag ttcgtggccg agctgcgcgg cctcaagggc tcgaagcgca
    10321 agcaggcggt gaaccgcgtg gtcgagctgt gcttcctcga ctcggtgctg cgccagtcga
    10381 tcgacaccct ctccaagggc tacaagcacc gcacctgcct cgcgcaggcg ctcatccacg
    10441 accccgaagt gctcatcatg gatgagccca ccgacggcct cgatccgaac cagaagcacg
    10501 aggtgaggaa cctgatccgc acgctcggca agaacaaggc gatcgtgttc tcgacccaca
    10561 tcctcgagga agtggacgcg gcgtgcacgc gcgcgatcat catcgaccgc ggcaagatcg
    10621 tcgccaacgg cacgccggaa gagctgcgcg acatgtccaa cctcgccggc gccgtcacgc
    10681 tttcggcgca tggagccagc gccgaaaagc tctctgctct tgggagagtc gaagacctgg
    10741 gcggcgcgtt ccggatctat ccgaacgaca agtcccagtc ggccgagctc gcgcgcggcg
    10801 tggtcgagct ggtcgcgcgc gaaggctgga aggtcgaagg catgttcagc gagcgcggcg
    10861 agctcgacga agtgttcagg cgcatcacgc tccccgacac ggtgaagcga tgaggatcat
    10921 ctggaccatc gccaagcgcg agctgagcgc gtacttcacc tcgccggtgg cgtacgtgtt
    10981 cctggtcatc ttcctgctcc tcaccgggtt cttcaccttt accgcaggca acttcttcga
    11041 gcggggagaa gcgagcctgg cggccttctt cggctggcat ccgtggctgt acctggtgct
    11101 ggtgccggcg gtgggcatgc gcgtctgggc cgaagagcgc cgctccggga cgatggagct
    11161 cctcctcacc atgccggtcg cgacctggca ggcgatcgtc gccaagttcc tcgcctcctg
    11221 ggtgttcctc gcgctcgcgc tcgccctcac cttcccgacg atcctcaccg tgaacgtgct
    11281 gggcgatccc gacaacggca tcatcgcggc gggctacgtc ggcagcctgc tcctcgccgg
    11341 cgcatacctc gcaatcagct gcatgacctc ggccatgacg cgcaaccagg tggtcgcgtt
    11401 catcctttcg gtcgtggtgt gcctgttcct gatcctcgcc ggcttcaacc cggtgaccga
    11461 cctgctgacg cgctgggcga gcccgcaggt ggtcgacacg gtcgcggcgt tctcggtggt
    11521 cacccacttc gacgcgttcc agcgcggcgt gatcgacggc cgtgacctga tgttttttct
    11581 ctcggtgatc ggcttcgcgc tgtttgcgac cggcgtgatc attcgcggac accgctcagg
    11641 atgaggaaat acgaacacct gatttactcg gcggtcgggc tgatcgcgct cttcctgctg
    11701 ctggtggcgg tgaacttcct ggtgtcgcgg gtcccggcgc gcctcgacct cacgcagggc
    11761 aacctctaca cgctctcgcc gggaaccaag aaggtcctgc gcggcctgca ggccccggtg
    11821 aaggtgaagc tctacgtctc gcagggcgag gcggtaccgg tgcagctgcg cggcttcgcc
    11881 cagcgcgtcg aggacctggt acgcgaattc aagagcgtgg cgggatcgaa cctcgtcgtc
    11941 gagcgctaca acccgaggcc cgattccgag gaggaggacg ccgcgcagct cgacggcatc
    12001 gagccgcagc agctcttcag cggcgagcag ttctacctcg gcgtagcggt gaaccagctc
    12061 gaccgcaagc agtcgatccc ggcgatctcg ccgcagcgcg agcgcctgct cgagtacgac
    12121 ctggtgcgcg cgatcgcgcg cgtcggcgcg accgagcgcc cgaagatcgg cctgatggcc
    12181 ggactgccgg tgctcggcga gcgcttcaat ccgttcacgc gccagtcctc ggagccctgg
    12241 gtgctcgcca acgagctcaa gcgcgagttc gaggtcaagg aagtcccgct gaccgcgaaa
    12301 gagatcgaca aggacctgaa cgtcctgctt ctcatccatc cgcgcgacgt gccgccctcg
    12361 accgaatacg cgctcgacca gttcgtgatg cgcggcggca agctcatcgc tttcgtcgac
    12421 ccgtacgcct acttcgacca ggggccgacc atgccgggca tgccgccgat gccgtcgagc
    12481 tcgaccctgc ccacgctctt caaggcgtgg ggcctgagca tggagccggg caaggtgatc
    12541 gccgacgtgg tgttcggctc gggtggcggc gcgcgctaca cgcccaccgt actctcgctc
    12601 aaccgcaccg cgttcagccg cgacgacatc gtcaccgggc agatcgagac cttgctctat
    12661 gccttcggcg gcgccttcga gctcaagctt cccgagaacc tgaaggcgac cgagctggtg
    12721 aagagctccc ccaactcgat gctggtggac gccatgaacg cgaccaagtc gggcgacgag
    12781 gcgacgcgcg cgttcaagcc ctcgggcaac gcgatggcgc tcgcgctacg gctcaccggc
    12841 aagttcaaga cggcctttcc cgacgggctg aaggaagacg agaagaagcc cgccgcgcaa
    12901 ggaacgccgg cgctgcgcga gtcgccggag aactcggtca tcgtggtggc cgacgtggac
    12961 atgctcgccg acggcgccgc ggtcgacgtg caggacgtgt tcggccgcaa ggtcgtcgtg
    13021 ccctcgaacg gcaacctcgc gttcgcgctc ggcatggtgg agcagttcgc ggcgggcgac
    13081 gagctgatct cgctgcgcag ccgcgccgcg tcgttccggc cgctcaccgt ggtgcgcgag
    13141 ctcgaggccg aggcgcagaa ggagtatttc ggcaggatcc aggcgctgga ggaggaaaag
    13201 cagaagacgc aggcgcgcct ccaggagctg cagaaggccc aaggcggggc ggcgaaaggc
    13261 gcgcagctcc tgagcgccga gcagcaggcc gagctcgagc gcttcaagaa gacctacatc
    13321 gagaccaacc tcgcgctgaa ggagctgcgc aagaacctgc gccaggacgc cgaggcgctc
    13381 gtgttctgga ccaaagtggc gaacatcgcc ttcatgccgc tgctggtggc gctcctcggg
    13441 ctgctgatcg cctttctgag aaaacgggct gcatgaacgc gcgggtcgcc gcgatcctgg
    13501 tcgttctcct cgtggtgctg gggggcgggg cgctgctcta ccagcagcag gagcgcacgc
    13561 aacgcccgga gaatgtggcg acgctcggcc gcagcctctt caaggatctc aaggtcgccg
    13621 acatcgcggt catccgcatc gtcgagccaa agggcgcgct cacgctgcag cgcaaggacg
    13681 atcgctgggt gatcgccgag cgaggcgagt tccccgccga cctttcccgg gtgcgggagt
    13741 tcgtgctgaa ggtgatcggc ctcaaggtcg gccagagcga gccgctgggc gagaaggacc
    13801 gggcgcggct caatctcgat gcgtcaggca cgaaagtcga attcaaaggc gccgacggca
    13861 aggacctcgg cgtcttcgtc gtcggcagga agtacttcaa gcgggaagtg gagaacccgg
    13921 agaaggcgaa ccccgacggc cgcttcgtcg gcctgccgca ggaggccggg accgcctacc
    13981 tggtcggcga ccccctcacc caagccacgg tcaagaccgc cgaatggatc gaccgcagct
    14041 ccttccaggt ggagaaggtg aagaccctcc aggtgcgcta tcccggggga gagggctggc
    14101 gcattgaccg gcgggcggac aacgccgaat ggaagctggc gaacgccaaa ccgggcgaaa
    14161 agctcgacat ttcaagggct aatagcgctt cctactcgct ctcgctgctc gagctcgccg
    14221 atgtcgcacc gaagggggac gccgacacgg gcctcgacaa gccgaccacc atcgacgcga
    14281 ccacgctgga gggtgcagcg tacgcgatca aggtcggcaa gctcgccggc gaaaactact
    14341 acgtcacttt cactgcctcc ggccacaagc ccgacgcgaa ggacgccgag cgcgtcaagc
    14401 tgctctcgca gcacgtgctc cttatccaga agtccaagct cgaggacacg ctgaaaaaac
    14461 gctccgagct actggagaaa aaagcaaaca agaaataaaa gcccttccta gcggaccact
    14521 tccggcggcc tgtcgtcgat agtccgtgta gcgcgtctcc tcttaccctt tcggcatctt
    14581 ttcggagagg agcgtttcac catgagtagg ggcatgaagc aactggcggc attcgccgcc
    14641 ttcgtctgcg tggccacgcc gagcgccgcg ttcgacactc tgtcgcagca atcgcccggc
    14701 gtgatgttct tcttcagcat acccatcgac gcacccaagg cgaagcacga gaccttctcg
    14761 gcgggcttcg cgatccaggg caagcgcgag tacgagaccg tgaagatcga ctcgcgcatg
    14821 ctcaacaact tcatcggcgg cgggatcgag gcgaaatgga tcatcgccgg ggtcgtggcc
    14881 gcggggaccg cggtcgcggt ggcgagcaag gacaagtcca ccaccaccag ccagcaacag
    14941 cagcagcagg cggcgcagca acaggcccag cagcaagccc agcagaccgg cggcggcacc
    15001 agtcccggca cgccctgccc cgtcaagccg aactgcccgt agcgacgccc atgtagcttc
    15061 gcgttcctcg aggcgaggct aaactggggg cgtggcattc gatccgcacg tccccgagat
    15121 tgcgggggtg atccagctcg cgatcgcccc cgttttcatg ctgacggcgg taggcacggt
    15181 gatcggggcg ctcaacattc gcctcggccg ggccgtggac cgccgccgcg agctcgagga
    15241 gcggctttcg gccatgtcgc aggcggagtt gccctccgcg caggaagagc tcggcacgat
    15301 tgcgcgccgc atccgcttcg tctacctttc catcgttttc gccgtcattt cagcgctgtt
    15361 catcgcgctg ctgatcgcgg gcgcgttcgt gggcgcgttc gtgcgtgctg acctgtcctt
    15421 caccatcgcc ggcatgttca tctgcgccat gctggccctg atcgtctgcc tgctgctctt
    15481 cctgagggag atcttcctcg cggtctcgac cccgcgccac gtgccgcggt aacattaatt
    15541 tcgaggaaga ggaggcaggc ccgtggccca ttcgatcaaa cgcattagga cgatggcgct
    15601 cgtcggccag tccggggcag gcaagaccac cctggccgaa gcgctgctcg cgaaagcggg
    15661 gaccatcccc gcggcgggca gcgtcgagcg cggcaccacc gtttgcgatt acaccccgct
    15721 cgagaagcag ctgcagcact cgctcaagct cgcggtcgcg agcttcgatt tccaggaaca
    15781 ccgcatccac cttctggaca ccccgggcta tcccgacttt ctcgggcacg cgctgccggc
    15841 gttcgccgcg gtcgagaccg ccgccatcgt gatcaacgcc cagaacggcc tcgagatgat
    15901 gaccgggcgc tttgtgcaat gggcggcgaa gcgcggcctc gatcgcctca tcatcgtcaa
    15961 caagatcgac gccgacaacg tcgacctgga ggacttgctc gagcgcatcc aggacgcctt
    16021 cggcaaggag tgtctgccgc tcaacctgcc ggccgccaac cgcaccaaag tgtcggattg
    16081 cttcttcgcg ccctcgggcg agtccgactt ctcgtcggtc gacgaggcgc accgcaagct
    16141 cgtcgaccag gtggtcgagg tcgacgagaa gctgatggaa gcctatctcg agaagggcga
    16201 agtgagccct gacgagctgc acgagccgct cgagcgcgcg ctgcgcgaag gccacctgat
    16261 cccggtggtg ttcacgtccg cgaaaaccgg cgccggcatc ggcgagctgc tcgacgtgat
    16321 cgtcaatctg ctgcccaatc ccaccgaagg caacccgccc tcctacgtcg tcaccaaggc
    16381 cggcaacggc cagggtaccg acctgacgcc gattcccgat cccggcaagc acgcgctcgc
    16441 gcacgtgttc aagatcgaga tcgacccgta catcggccgg ctcgcggtgt tccgcgtgca
    16501 ccagggccgc ctcacccccg gcggccagct cttcatcggc gagggcagga agccgatcaa
    16561 ggccgggcac ctctatctcc tgcgcggaaa gacccaggtc gaggtgcacg aagcgatccc
    16621 gggcgacatc tgcgccatcg ccaagatcga cgagatccag ttcgaccaca tcctgcacga
    16681 ctcggccgag gacgcgcacg tgcacgcccg tccgctcgag ctgcccacgc ccgtgttcgg
    16741 cctcgcggtg cagccgaaga agcgcggcga cgagcagaag gtttccgacg tgctgcacaa
    16801 gatcatcgcc gaggacgcct gcttccgcct cgagcacaac tcgcaggcca acgagatggt
    16861 gatgcgcggg ctcggcgaga tgcacctgag gaccatcctg gaaaagatgt cgacgcagta
    16921 caagctcgag ctcgacacgc ggccgccctc gatcgcctat cgcgagacca tcgccgcgcg
    16981 cgccgagggc cacagccgcc acaagaagca gaccggcggc gccggccagt ttggcgaggt
    17041 ctacctcaag atcgagccgc tgccgcgcgg caagggcttc gagttcgtcg acagcgtgaa
    17101 gggcggcgcg atcccgaacc agttcatccc gtcggtggag aaaggcgtgc gcggcgtgct
    17161 cgaccacggc ttcctcgccg gctatccgct gcaggatgtg agagtgaccg tgtacgacgg
    17221 caagcaccac ccggtcgact cgaaggacgt cgccttcgtc tccgccgggc gcaaggcgtt
    17281 cctcgacgcg ctcgccaagg cgcgccccat cgtgctcgag cccatcgtga acgtggagat
    17341 cgtcgcgccc gaggagcgca tgggcgacat cgcgggcgag ctctccggcc accgcggcca
    17401 gatcaagggc tccgacgcgc cgcgcccggg cctggtgcag atcaaggcga ccgcgcccct
    17461 ctcggagctc gagcatttcc cggcgcgcct caagtcgctc accgcgggcc acggcaccta
    17521 caacctcgag ttcagccact acgagcccgc gccgccgcag cttcagcaga agctcgtcgc
    17581 cgcgcacaag cccgcggtcg gcgcggaaga ctaggcctct tgcatttcgt gcgccagggg
    17641 cgcggcgccc cgcccctggt gttcgtgcac ggctttctct gcagccacga agactggaag
    17701 tttcaagtaa gagaactgca gagaaaccac gaggtcgtcg cctgtgacct gcgtggtcac
    17761 ggcgagacgc cgggacgccc gcaggaatgc tcgatcgagc attttggcgg cgacgttgcc
    17821 gcgctcgtca acaacctcga gctgccgaat ccgattctcg tcggccacag catgggctgc
    17881 cgggtggtgc tggaagccaa tcgaatcgcg cccgaacgca tcgccgggat cgtgctggtc
    17941 gacggcagtc gcttcagcgc gggagacccg gatgccgcgg aagcggccgc gcgcagccag
    18001 atcgagcaag tgggattcgc cgcctgggcg gaggagctct tccggcagat gttcctcacg
    18061 tccacgccgg cgtcggagcg cctggtcgcg cgcgcggtgc gccagggcgc cgagatcggc
    18121 gccgcgctgt ggccgcgatc agtgcgctgg gacgcaggcg cgctggaagc ggcgctcgct
    18181 gccgtgcgcg cgccggttct cgccatccag accacaacgc gcgacccgca gtcgggaaag
    18241 cgcggaccgc tcaaggccgg gcaaaccacg ccatggcttg atcttctaag aacaagaatt
    18301 ccaacgtgcc gcgtcgagat cctgccgggc ctcggccact ttccgcagct cgaggcgccc
    18361 gagcgcgtca accgcctgat cgaagaattc tgtcgatgaa gcggtgaacc ttcagcgcct
    18421 cggtaccgct cacgcggggc tcgcggttct gctcgatcga atccaggaag tccgccagca
    18481 cgccgcggtg ccagtcgtgc gggaaagcca tcgggtcggc gccggtgccg ccgccgccgg
    18541 attcggtctt catctcgaag gactgcgcgt cgctgaagct catcttgagc gaggtgccct
    18601 cgagcagcgc cgtgccctgc gtgccgatga gctcgatgcg ctccgggaag cccgggtacg
    18661 ccgccgtggt cgcatgcacc gtgccgagag cgccgttctt gaagcgcacc gcagcggccg
    18721 cgagatcctc ggtctccatc ttgtgcaccg gcgtcgtggc gacgaagctc ttcacttcgg
    18781 ccggctcacc cgtatacgag agaaacaggt ccagcgtgtg gattccctgg gtcaggagca
    18841 cgccgccgcc gtcgcgcgcg cgcgtgccgc ggcccggctg gtcgtagtag ctctgcggcc
    18901 gccagttggg aatcgcggcg gacgccgaga ccagtttccc caggagcggc agcatctcgc
    18961 gcagcttctc cgcggcggga cggaagcggt gctgcaggac catccctagc ttcatgttct
    19021 tgcaggattg gacgagacgt tcggccctcg cggtggagat ctccagcggc ttctcgagaa
    19081 ggacatgttt gcccgcggcg gcgcagcgct cgacgatctc cagatgcgtg ttcggcggcg
    19141 tcaacacgag caccgcattc accgagcgat cgtcgaggat ggtttcgagg cgatcgcact
    19201 gcgggaaggg aaagcgctcg gcgaagctct ttcttctggc ggctgaaggg ctgtaggcgt
    19261 acgcgacttc gacgcgcgac ctgagatcct gcaagctctt ggcgtgcggc gtcacggcca
    19321 tgcccaggcc gatcagcccc accttaaaag ccatagagct ccgcggggtt gtccaccagg
    19381 atcttcttgc ggtcgttctc gatgggcgcc cagttgagaa gtagatcgag cagggccgcg
    19441 tcgtccggcg gcgccggctt gcgcgcggag ggatgcggcc agttggaagc ccacagcatg
    19501 cgctcgggcg ccttcttcac cagcgctttc gcgaggcggc tgacgtcttc gtacttcggc
    19561 gcaccggtct tcgacgtctc gtacggcgcc gagagcttga cccagcagcg gccggtgtcc
    19621 accaggttca gcagtgaacg gaactcgtgc gactccggcg aaaccggctc gagaaacttg
    19681 ccggtatggt cgatgacgaa cttgccgggc agacgcttga tctgcgcttc gtatttcggc
    19741 agctcacggc cgtcgagctg gatgttggcg tgccagccga acgggtgcac gcgcgccatc
    19801 accgggtcca tcaaatcgaa acccagcatg ccgccgtgca gcgtcatgat gcgcactgcg
    19861 caaatgcccg ccttggtgag acgctcgagc tccgcatcgg caacgccggg cgtgaccacc
    19921 gcgacgccct tggcgctctc gccgagcttc cggatcgaat ccacgatgac gctgttgtcg
    19981 tccttgtagg cattcgcctg cacgacgatg acacgctcga ggccgagctt cttctgcagc
    20041 gcgcggtagt cctccaccgg gaagtggccc ggcagaaaag tgcccggcgc ggcgggcagc
    20101 gcctggtcgt agaagtgcat gtgggtgtcg caggcaccct tcggcaccga gatcttcact
    20161 tcttctttcc ttttttcgcc tttggcttct tcggcggtgc tgcgggattc ggcacttcct
    20221 cggcggtgca gaagattccg ccctggtgcg cctccgcgat cgcgtcgctg cccggcttct
    20281 ccttgcgcag gatctcttcg gcgaacggct cggagtcgtc ctgcggccgg tagccgaggc
    20341 gcgtggcgtt ggagttgtcg taccagttgc gcttgttgcc cgagaccccg tagacgatct
    20401 cgaacttgat gcccgggtgg tcgatgccga tcgagacgag ctgcgcgatg tcgcgcgggc
    20461 tgatccacag cgagagccgg cgcttgtcga gcggcgccgg gttcacgttg ccgatgcgga
    20521 ccatgaggaa ctccaggccg tacttgtcgg catagagact gccgagcgcc tcgccgaaca
    20581 ccttggagac gccatagcgg ccgtcgggct tcggatagac ccggtggtcg atcgtctggt
    20641 cgcggcgata gaagccggtg gcgtggttgc tggtcgggaa gacgatgcgc ttcacgccgt
    20701 ttctgcgcgc cgcctcgaac acgttgtagc agccgatgat attcgcctgc aggatcgctt
    20761 cccacggccc ttcgaccgag tacccgccga gatgcacgac cgcgtcgacg cccctggtga
    20821 tgcgcagggc atcggccatt ttcgagatgt cggccttgat gaagttcttt gccttgagcg
    20881 gccgccggtc ggaaaggcgc agcttgtatt tcgccgcgag ctcgcgggcg agatgcgtgc
    20941 ccacgtctcc ggtggcgccg gtgatgagaa cggtcttcac aggactctcc caatgatgtc
    21001 gttgaactgc gccgcgcgct cgaaatagac cgagtgcccg gcggccggaa cccggaccgc
    21061 gcttcccttg ggcgcgatgc ttgcgagcgc cgggccggcc gcgggcggaa atacccagtc
    21121 ttcgtcgccg gtgatgaatt gaaccggcat ggacagcttg gccagaaggg aaggcgtctg
    21181 ggtgcgcaga tccctgatgc gcacgcgcac gtcctcgcgg aacgatgccg gagccatctc
    21241 cgagatctgc cagtagagct gcgcgagcgc cggctgctcg cgcgccatgc gctcgccgcc
    21301 cgcggggtgg atgccgcgcg actgaagctc ggcgagcccc gccggagatc ggtcaatcca
    21361 ctcgctgatc tccgcgtttt cgagatgact gaaatcgagc gcgcccgacg tcgaagccat
    21421 gacgagcgcc cgcaccttcc tgggctcgcg cagcgcatat tcgagacagg tccagccgcc
    21481 catcgactgc gctacgagag ccacgtcttt cagatcgagc tcccggatca gggccgcgag
    21541 gtcgtcggca tacgaatcgg gagcgctgct cccgggcacc gggctggaag gcggaaagcc
    21601 gcgatgcgcg aaggcaacgc aggtgtaccg gggggcgaag tgcgcgatct gctgccacca
    21661 cgagagatgg ttgccgccca gcccgtgcgc gaagatgacc gcaggtccct cgccgacggc
    21721 ttcgtaatag atctcggcgc ctgctccttt tcccccttct tgccgcaacc ggccggctcg
    21781 tctcacggct ttcgtttcca gtgctcggcg ttcacggcgc ccttgggaac gcggcctttg
    21841 acgatctccg ccgcctgcgc caccgtctcg agcgactggt gctcgatcgc cggcatcgtc
    21901 aggcccgcgg tgtgcggagt ggcgatcacg tcggggcgcg ccgcgagccg cggcgtcggc
    21961 atctggtcgc tcgcgcggcc gacgtccatc gcgcagccgg cgaggcgccc ggaatcgagc
    22021 gccgcctcga gcgccgcctc gtcgaccagg ttgccgcgcg agacgttgat caggcaggcg
    22081 ccctttttca tcttgaagaa tgcttcggca ttcatcaggt tctcggtctc cgcggtagcc
    22141 accgcgaggg gcacgacata gtcggactcg cgcaaaagcg tgtcgagcgg cacctggtcc
    22201 acctgctgat agggatcgtg aacgaggacg cgcatcccaa gcgctcgtcc cattcttgtg
    22261 acctcttttc cgatagcgcc gtagccgatg accccaagcg tgctgccctt cagctcgcgg
    22321 cccatcgtcg ccttcggcac ccggttctgg tggtaaaggg tcgccgagcg cgtgatcccg
    22381 cgcgaaaggt cgacgagaaa tcccagcacc atctcggtga ccgaggtgat gaagcccgcg
    22441 ctcgcctggg tcaccagcac gccggccttg ctcgcggcgg cgacgtcgac attgcggatg
    22501 tcgatcgcgc agcgcagaaa ggcgatcagg cgcggcaggc gctcgaaaac cgcggcagga
    22561 ccgggcgact ggcgatagga aacgatcaga tcgcagccct cggcggcagc gatcagggct
    22621 tcgccctcca acgagtcgga tttttgattc aacttcacgt cacccacggc tcgcaggccc
    22681 gcgagcgcgc gctcgccgta atagttagcc atcgcctccg gcgaatgggt cagcaggatc
    22741 ttcaagcggc ttttttctgt tccgcggcga gcttgcgata gcgctcgagc gagcgcgtga
    22801 ggtggcggcg catcgcttcg cgcgcgcctt tggcgtcggc gctctcgatc gcctggtaga
    22861 tccggcggtg ctcttcctgg atgagcgcga ggtaggcgcg ctcgccgccc atgcgctcgg
    22921 gcagcccgct caccgtgcgc cgcgggatga ggtggcggcc gatgaactgc aggaagcgcg
    22981 gaaactcggg attgccggtg gcctcggcga tcgcgcggtg gaattccagg tcctcgtcga
    23041 ccgccgagcg gccggcgacg ctcgcctctt caatggcatc cagggcgttg gaaatcgtgc
    23101 gtacctgggc tttcgaggcg cgatctgccg ccagccccgc cgactcgatc tcgaccccga
    23161 ggcgcagctc catgacggcg aggatatcgt cgagcgtctg catgcgctcg ggctcgatgc
    23221 ggaagggcgc gcgctgcggc tcggccgaca cgaacgcgcc gacgccctgg cgcgtcacta
    23281 ccaacccctc ggcgcgcagc gccgcgaccg cctcgcgcac cacggtgcgg ctgacgcccg
    23341 cggcctgcgt cagcgcctgc tcggtgggca ggcgcgcgcc cggagcgaag cgcccgctgc
    23401 ggatctgctc ggcgagctgc tcgaacagcc gccgcgacag cttgcgctcg gcggcgacgg
    23461 ggcggatgcg catgggctgg aggcgagcct tgacggcaaa tgctgcggtt gtatgatgac
    23521 tgacgaatct tatcagagga ggattcatgt tccgtcgtca tttcatcggc gcggccagcg
    23581 tggccgcggt atccgtttct ctattgagcg cactgcccgt gcaggcgcag cagatcatca
    23641 aggccaccga cgtgcacccg ctcggctatc cgacggtcga ggcgctggtg cgcatgggca
    23701 acaagctcga gaaggcgacc aacggcaagt acaagatgca gatgttcccg tcgatgcagc
    23761 tcggcggcga gaaggagatg atcgagcagg cgcaggtcgg cgcgctgcag atcgcgcgca
    23821 tctcggtcgg cccgatggga ccgatcgtcg acgagctcaa cgtcttcaac atgccgttcg
    23881 tgttcaagga cgaagcgcag atgcgcaagg tgatcgacgg cccgatcggc gaggagatgc
    23941 tcaccaagct ctccaacagc tcggcgcgcc tgatcgcgct cggctggatg gacgccggca
    24001 cgcgcaacgt ctactccgac aagccggtga ccaagcccgc cgacctgaag ggcatgaaga
    24061 tccgcatgat gggcaatccg ctgttcgtcg agac
//
LOCUS       DATBSZ010000298        12263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_608655, whole genome shotgun sequence.
ACCESSION   DATBSZ010000298 DATBSZ010000000
VERSION     DATBSZ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12263
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608655"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VIG70_19665"
     CDS             <1..302
                     /locus_tag="VIG70_19665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252815.1"
                     /translation="GTCPTAGFITREISRQARENSASCLLVDVRGLRDRAGRLREVLA
                     SRSIPGRIAVVDSAANDRIYQFAQIDAANRGCALQRFEDPDAALKWLWEFPSSGR"
     gene            complement(265..1407)
                     /locus_tag="VIG70_19670"
     CDS             complement(265..1407)
                     /locus_tag="VIG70_19670"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY8252816.1"
                     /translation="MIFDPAQIQRGARLRRSPYFEATQRAGCRGYTVYNHMLLPIRYD
                     SFEAEYRRLLADVTLWDVSVERNVEIAGRDGLRFAELLTPRDLSKCRVGEGRYVLLTD
                     DAGGIVNDPVLLRLEENRFWLACADSDVLLWAKGVARNSGMDVELRELDVAPLQIQGP
                     KSRDLVSMLFGARVLNLGYYHFFETKLDGIPVIVTRTGWTGELGYELYLLDPARGVDL
                     WKRVLKAGKRYNIAPTGPSDIRRIEAGILNYGIDMTLDNNPYELGLERLVNLEKAADF
                     IGRDALRKIRTDGAQKKLVGVEIGGSKMDLNMTRYPVRVEGKTAGFVTSCVYSPRLKK
                     NIGYAMLPIEKASAKKLVVEAPKGPRSAVVVPMPFIDPKKEIPKAT"
     gene            1482..2720
                     /locus_tag="VIG70_19675"
     CDS             1482..2720
                     /locus_tag="VIG70_19675"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8252817.1"
                     /translation="MQKLLGKGATGTVYLAKDTFTGKEVALKTIEPEVFRDPEFGTVY
                     RSQFLNEASLAGKLRHPYIVSILDAVVGEDSGHIAMELVTGGDLSKHATPDTLLPVPD
                     VLQIGFKCCGALEYASKEGIVHRDIKPANIMLAGGTEVKISDFGAAVLKKSQVVQSAA
                     LGSPYYMSPEQIEGKALTFHSDMYSLGVVLYELLTGKRPFVAEKIDALIAKILKADPS
                     PPSAVRQGLPKELDAVVLRALQKNTAHRYATWSEFALELSRVGEAVLPPGAIPDSEKY
                     VTLKKVPMLSTLADSELWELARAGRWTRVAKGKPVLQEDERGKSFFFLAKGEAKVIKR
                     KKLLNMVNGGECFGEMAYIGGGEQPRNATVEAVTELLLAEFEPSALDKMSLGAQLQLT
                     RALMRNVTDRLALANIRLAR"
     gene            2689..3561
                     /locus_tag="VIG70_19680"
     CDS             2689..3561
                     /locus_tag="VIG70_19680"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HEY8252818.1"
                     /translation="MPSPTSGSPGKTVKVDAPAWLVRFFTPFIGRWAARDLSAKYPGL
                     GPQEIREEMREKIFAERGRAPTEVEERMLDAMLERLPEKGQAASVEKPVSAWVLVAAN
                     LVPLAGVLFWGWDAFALIALFWMENVVVGVFFVARMLCADPRDPALWAAKLVMVPFFG
                     FHYGMFTAIHGVFVFDLLGGKRYDVQGLWVLEPAARAAADYGLWLPVAVLVASHFFSF
                     AWNYLVRGEFRRAKMSELMTKPYVRVVVLHVAIILGGIGAMALGSPLWALLVLLAMKI
                     GLDLKAHVKEHSAP"
     gene            3558..4355
                     /locus_tag="VIG70_19685"
     CDS             3558..4355
                     /locus_tag="VIG70_19685"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY8252819.1"
                     /translation="MKSKRIAILESRLGEQLVSLVSQRGGVPFHAPALAELPDLEPQK
                     IAELMRALEQRPPKLFVFQTGVGTRALFAALDGLKQTQLFLRLLDQSIVVVRGPKPTG
                     ALRARGVRIDSSAADPFTTKEVLAAIEGIALKGEGVIVQRYGVANAELDRALEARGAQ
                     VTEIPTYRWSLPADTQPLEQLIGALERNEMHAAVFTNAEQVRNLFAVARKLDKAAVLQ
                     QSLNRTLVASIGPVASAALREYQVKIGLESKPPKLGALMTALDSALS"
     gene            4352..5308
                     /locus_tag="VIG70_19690"
     CDS             4352..5308
                     /locus_tag="VIG70_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8252820.1"
                     /translation="MKIAFLALLLATQALAQPTYPSKPIRLVVGFPPGGAADFVSRAF
                     QEPLGRALGQPIVVENRPGAGSSIAAEHAARSAPDGYTVLIASPSSILVNPVISPKPG
                     FEPLKDLIPISRVSSSPLILAVNPGTGIDSVRELIAHAKKHPGTLNFATSGNGSAPHL
                     AAVLFMRLAGVEIVHVPYKGGAPAVQSVLAGDTQLAFATPPSVLPLVQAGRMRALATT
                     SRAATPLIPGIPGMGEAGLPDYEISFWYGLFVPEGTPAAVVRTLFAAIKQVVKAPETA
                     RILAREGTEAVGSGSPDEFAIFLEQDAKLWARLVKESGAKAE"
     gene            complement(5316..5657)
                     /locus_tag="VIG70_19695"
     CDS             complement(5316..5657)
                     /locus_tag="VIG70_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252821.1"
                     /translation="MTRLLAALLGALVAGSALAAPKAESAVECGIAADMAVVARSLAE
                     EEVQRPQAGAIMARIYDVSESSRGKELMKDILDAAYRTNGATSQKFAEDLFAACLKTG
                     GNMDTILGRRL"
     gene            complement(5663..5983)
                     /locus_tag="VIG70_19700"
     CDS             complement(5663..5983)
                     /locus_tag="VIG70_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252822.1"
                     /translation="MNLKTVMTLAMAAVFTTPVLAQQTGSGQEPRDTNTATPSVRGFP
                     AMDRNNDGYISRDEARDAPWSNRFSEMDKDNDSRLSLGEYDAMVQQGAAAGATRNERD
                     FRKE"
     gene            6050..6790
                     /locus_tag="VIG70_19705"
     CDS             6050..6790
                     /locus_tag="VIG70_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8252823.1"
                     /translation="MELGIQGRTALVTGASMGIGRGIATALAREGVKLAVVARRRNLL
                     EALESELKTKLVIIEQDFLKPGAPESIAEQALAGLGSIDILINNAGGSRKFTLDSSEE
                     QWEEALTLNFTRQRQLAHRLLPQMIAKKWGRIVNITGKSEPEGLNGAFAAKAAMHSWA
                     KGLSREVGKHGITVNCIPPGRIISEQILRNYPPEYRKKQSEEEIPVGEYGQPADIANL
                     VCFLASPLARYITGAVIPVDGGLRRYQF"
     gene            6800..7750
                     /locus_tag="VIG70_19710"
     CDS             6800..7750
                     /locus_tag="VIG70_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8252824.1"
                     /translation="MRIFLLALFFACTAHGQTYPNKPIRLVVPFAPGGSSTIVARSVA
                     AEMEKALGQSIVIENKGGGGGNLAMHDVARADADGYTLIIGHVGSLAMTPYITPNLGY
                     DVNKDFAPVSLLAKVPNIFVVHADVPAKNLREFVALAKAKPGQFYYGSAGNGSAGHLA
                     MEYLKLVSGMDLQHVPYKGTGPNLVDLVAGRTHATSAGTPPLMPHVKSGKLRVVAVGT
                     PQRLASLPDVATVAEQGYPGFETSQWYGLNAPAKTPPAIIKRLSDEAAKAVKSAGVIE
                     RFKADDAEAVGSSPQDYAQFISKEQARWKEVIQKAGVKAD"
     gene            7750..9234
                     /gene="tcuA"
                     /locus_tag="VIG70_19715"
     CDS             7750..9234
                     /gene="tcuA"
                     /locus_tag="VIG70_19715"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEY8252825.1"
                     /translation="MVDFDVIVVGGGNAALCAALSASESAKRVLVLERAPEEEAGGNS
                     RFTAGLFRLVYTGPEDLKTLIPDLSPEEIANTDFGTYTEGQFLDDMARLTEYRCDPDL
                     TEILVKESFPTVKWMRQKGVRFTAAWGRQAFRIGGKFKFWGGLTVEAVGGGPGLVESE
                     TQLAKKAGIEIWYQARAVALIADDDGVHGVVVKKAGKTLQAKAKAVVLAAGGFQANTE
                     WRTRYMGPGWELAKVRGTRFNTGDAIEMALDIGAAAHGNWSGCHAVAWDRNAPETGDL
                     AVGDGFQKHSYPWGVYINADGRRFVDEGADFRNYTYAKYGRVILQQPKQFAWQIFDAK
                     VKRQLRDEYRIKQVTKITANTLEELVSKMDDTDQKAALETLKKYNAAVRTDIPFNPNV
                     KDGRCTENLPVNKSNWANTLDTPPFEAYAVTCGITFSFGGLKINSQTQVLSSDGEPIA
                     GLYAAGELVGGIFWFNYPGGSGLTNGAVFGRIAGANAASASRER"
     gene            9236..10474
                     /locus_tag="VIG70_19720"
     CDS             9236..10474
                     /locus_tag="VIG70_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252826.1"
                     /translation="MERKMTPVASRGIKFVGHSDMGLHGDGVQVMVHRGHAYVGHGYS
                     NGITTLDVRDPKNPKVTDFIACAPGTRAFHLQTHDDLLLAVNAPSVWTMQQFQTEKDY
                     FGGSPADRLKGTGVQFSAGIRVYDISRPEKPREIGFMPTDGMGPHRIWYVGGRYAYAS
                     IHFADFTDHVLAVIDMSDPRKPQVVGRCWLPGMWRAGGETPTAPAGKRYALHHALLDG
                     DIAYAAWRDGGLTVINVADPARPKILVHRLPQPGGGGTHSPLPLPDRKLLVLADEPTF
                     ANCKEGRRHIWTYDVREPSNPAVIASFPEPGEADYCAKGGFFGPHNLHENRPGAFQSS
                     RLIFATYYNAGVRVYDIADQARPKEIAFYVPPNPAKMVDPRPNRPQVLQCGDCYVDRN
                     GFMYLTDQNAGLNILQFEGL"
     gene            10488..11624
                     /locus_tag="VIG70_19725"
     CDS             10488..11624
                     /locus_tag="VIG70_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8252827.1"
                     /translation="MKANWLGAALILGAALGTAHAQQTVFIPIVIEVSGSGAVSGTNF
                     RDGALMAIDEVNAKGGILGRRINAPVSDTQTNPGISRAQVQKALDGNPYAILGPVFSG
                     SVKVNMLLAQAAEIPQFTGAEAAEITQMGNDYIFRTSFGQQMSMPKMAVFVADGIKAK
                     SVAFLWVNNDYGKGGRDVFFKEMAKRNIKIVADVSTEAGQVDFAADIVKLKSANADAI
                     FIYTNEEECARVLREARKQAVKTPIIGETNVISQKVIDLAGDTAEGVRGHVGLTADAP
                     IPAVQAFTEKFQKRYKYVPDHNGIKGYTAVYAIKHATEKLGKFDSKALAKLMKGLTIT
                     PQQEPGILMEATWDAKGDIDRISFLVEVVKGRQKVVEVLPKLGK"
     gene            11701..>12263
                     /locus_tag="VIG70_19730"
     CDS             11701..>12263
                     /locus_tag="VIG70_19730"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8252828.1"
                     /translation="MLLWQTSQTINFAQGEFVMLPAFFVLAAMKFGGLSFPAALLVGL
                     LVALLVLGLLFKRLVVDPILRHGVLPLVISTLGLAILMKEGVKEFYAAEAQAFPGVWP
                     TTNVSIGGAVISAQSLVIIAVAVVAVGLLQYFLGATRTGRQMQATAQNPLLARVLGIP
                     VERMILYTFLVNAALVALASFLVSPIYL"
BASE COUNT         2125 a         4036 c         4085 g         2017 t
ORIGIN      
        1 ccggaacctg tccgacggcg ggcttcatca cgcgcgagat cagccggcaa gcgcgcgaga
       61 actcggcgag ctgcctgctc gtcgacgtgc gcgggctgcg cgaccgcgcc gggcgcctgc
      121 gcgaggtgct cgccagccgc agcattccgg gccgtatcgc ggtcgtcgac agcgcggcca
      181 acgaccggat ctaccagttc gcccagatcg acgccgcgaa ccgcggctgc gccctgcagc
      241 gcttcgagga ccccgacgcc gcgctcaagt ggctttggga atttccttct tcgggtcgat
      301 aaacggcatc ggcaccacca cggcgctgcg cggccccttc ggcgcctcga ccacgagctt
      361 cttcgcagac gctttctcga tcggcagcat ggcgtagccg atattcttct tgaggcgcgg
      421 cgagtacacg cacgacgtga cgaaacctgc cgttttcccc tccacgcgga ccgggtagcg
      481 ggtcatgttg aggtccatct tcgatccgcc gatttccacg cccacgagct ttttctgcgc
      541 accgtccgtt cgtatttttc gcaatgcatc ccgaccgatg aaatccgcag ccttttcgag
      601 gttgacgagc cgctccaggc cgagctcgta gggattgttg tcgagcgtca tgtcgatgcc
      661 gtagttgagg atgccggcct cgatgcgccg gatgtcggag ggcccggtcg gcgcgatgtt
      721 gtagcgcttg cccgccttga gcacgcgttt ccacaggtcc acgccgcgcg cggggtcgag
      781 gaggtagagc tcgtagccga gctcgccggt ccagccggtg cgggtgacga tcacggggat
      841 gccgtcgagc ttcgtttcga agaaatggta ataacccagg ttcagcactc tcgcgccaaa
      901 gagcatgctc accaggtccc gtgacttcgg tccctggatc tgcagcggcg cgacgtcgag
      961 ctcgcgcagc tcgacgtcca tgccggaatt gcgcgccacg cctttcgccc acagcagcac
     1021 gtcgctgtcg gcgcaggcga gccagaagcg gttctcttcc aggcgcagca gcaccggatc
     1081 gttgacgatg ccgcccgcgt cgtcggtgag cagcacgtag cggccttcgc cgacccggca
     1141 cttcgaaagg tcgcgcggcg tgaggagctc ggcgaacctc agcccgtcgc ggccggcgat
     1201 ctcgacgttg cgctccaccg agacgtccca tagcgtgacg tcggcgagca gccggcggta
     1261 ctccgcttcg aagctgtcgt agcggatcgg cagcagcatg tggttgtaca ccgtgtagcc
     1321 gcggcagccg gcgcgctgcg ttgcctcgaa atagggcgag cgcctgaggc gtgcgccacg
     1381 ttggatctgg gcgggatcga atatcatcta gtcatggtgg tgggcgaggc gacgcgcgtc
     1441 aatccgtaat ggatctcagc cttcaggttg ggagatatga cgtgcagaag cttctgggta
     1501 aaggggcgac cgggaccgtg tacctggcga aggacacgtt caccggcaag gaggtggcgc
     1561 tcaagacgat cgagcccgag gtgttccgcg accccgagtt cggcacggtg taccgctcgc
     1621 agttcctgaa cgaggcttcg ctcgccggaa agctgcgcca tccctacatc gtgagcatcc
     1681 tcgacgcggt ggtcggcgag gactcgggcc acatcgcgat ggagctcgtc accggcggcg
     1741 acctttcgaa gcacgccacg cccgacaccc tgctgccggt gcccgacgtg ctgcagatcg
     1801 gcttcaagtg ctgcggcgcc ctcgagtacg cgtcgaagga aggcatcgtg caccgcgaca
     1861 tcaagccggc gaacatcatg ctcgccggcg gcaccgaggt gaagatctcc gacttcggcg
     1921 ccgcggtgct gaagaaaagc caggtggtgc agagcgcggc gctcggctcg ccctactaca
     1981 tgtcgcccga gcagatcgaa ggcaaggcgc tcacgttcca cagcgacatg tactcgctcg
     2041 gcgtggtgct ctacgagctg ctcaccggca agcggccgtt cgtcgccgag aaaatcgacg
     2101 cgctcatcgc caagatcctc aaggccgacc catcgccgcc gagcgccgtg cgccagggct
     2161 tgcctaagga gctcgacgcg gtggtgctgc gcgcactgca gaaaaacacc gcgcaccgct
     2221 acgcgacctg gagcgagttc gcgctcgagc tttccagggt cggcgaggcg gtgctgccgc
     2281 ccggcgcgat cccggacagc gagaaatacg tcactctcaa aaaggtgccg atgctctcga
     2341 cgctcgccga ttcggagctg tgggagctgg cgcgcgccgg gcgctggacg cgcgtcgcca
     2401 agggcaagcc ggttctccag gaggacgagc ggggcaagag cttcttcttt ctcgccaagg
     2461 gcgaggccaa ggtcatcaag cggaaaaagc tgctcaacat ggtgaacggc ggcgagtgtt
     2521 tcggcgagat ggcctacatc ggcggcggcg agcagccgcg caacgcaacc gtcgaggcgg
     2581 tgaccgagct cctgctggcc gaattcgagc cctcggcgct ggacaaaatg agcctgggcg
     2641 cccagctcca gctcacgcgc gcgctgatgc gcaacgtcac cgaccgcctt gccctcgcca
     2701 acatccggct cgccaggtaa gaccgtcaag gtcgatgcgc cggcctggct ggtccggttc
     2761 ttcacgccgt tcatcggacg ctgggccgcg cgcgacctga gcgcgaagta ccccggcctc
     2821 gggccgcagg aaatccggga ggaaatgcgc gagaagatct tcgccgagcg cggtcgcgcg
     2881 ccgaccgagg tggaagagcg gatgctcgac gccatgctcg agcgccttcc cgaaaaggga
     2941 caggcggcga gcgtcgagaa gccggtgtcg gcatgggtgc tggtggccgc caatctcgtg
     3001 ccgctcgcgg gcgtgctgtt ctggggctgg gacgcgttcg cgctgatcgc cctcttctgg
     3061 atggagaacg tggtggtcgg cgttttcttc gtcgcgcgca tgctctgcgc cgacccgcgc
     3121 gacccggcgc tctgggccgc aaagctcgtg atggtgccgt tcttcggctt tcactacggc
     3181 atgttcaccg cgatccacgg cgtgttcgtg ttcgacctgc tgggcggaaa gcgctacgac
     3241 gtgcagggtc tgtgggtgct cgagccggcc gcgcgcgcgg ccgcggatta cgggctgtgg
     3301 ctgccggtgg ccgtgctggt ggcgagccac ttcttctcgt tcgcctggaa ctacctggtc
     3361 cgcggcgagt tccggcgggc gaaaatgtcc gagctgatga cgaagcccta cgtgcgcgtc
     3421 gtggtgctgc acgtcgccat catcctcggc ggcatcggcg ccatggcgct cggctcgccg
     3481 ctctgggcgc tgctcgtgct gctcgcgatg aagatcgggc ttgacttgaa ggcgcacgtc
     3541 aaagaacact ccgccccgtg aagtcgaagc gcattgcgat tctggaaagc cggctcggcg
     3601 agcagctcgt cagcctggtg tcgcagcgcg gcggcgtgcc gttccacgcg ccggcgctcg
     3661 ccgagctgcc cgacctcgag ccgcagaaga tcgccgagct gatgcgcgcg ctggagcagc
     3721 gcccgccgaa gctcttcgtc tttcagaccg gcgtcggcac gcgtgcgctt ttcgccgccc
     3781 tggacggatt gaagcagacg cagctcttcc tgcgcctgct cgaccagtcg atcgtcgtgg
     3841 tgcgcggccc gaagcccacg ggcgcgctgc gcgcgcgcgg cgtgcgcatc gacagcagcg
     3901 cggcggaccc gttcaccacc aaggaggttc tcgctgcgat cgagggcatc gccttgaaag
     3961 gcgagggggt gattgtccag cgctacggcg tcgccaacgc cgagctcgac cgcgcgctcg
     4021 aggcgcgagg cgcgcaggtg accgagatcc cgacctatcg ctggtcgctg cccgcagaca
     4081 cccagccgct cgagcagctc atcggcgcgc tcgagcgcaa cgagatgcac gccgcggtgt
     4141 tcaccaacgc ggagcaggtg cgcaatctgt ttgcagttgc aagaaaactg gacaaggcgg
     4201 cggttttgca gcaatccttg aatcgcaccc tggtcgcctc gatcggcccg gtggcctccg
     4261 cggcgctgcg cgagtaccag gtgaagatcg gcctggagtc caagccgccc aagctgggag
     4321 ccctcatgac cgcactcgac tctgccctat catgaaaatc gcgtttctcg cattgctgct
     4381 cgccacccag gcgctggcgc aacccaccta tccctcgaag cccatccgcc tggtggtggg
     4441 ctttccgccc ggcggcgcgg cggacttcgt gtcgcgagcg ttccaggagc cgctgggacg
     4501 ggcgctcggc cagccgatcg tagtggagaa ccggcccggc gccggctcga gcattgccgc
     4561 cgagcacgcg gcgaggagcg cgcccgacgg ctacaccgtg ctgatcgcga gcccgtcgag
     4621 cattctggtg aatccggtga tctcgcccaa gccgggcttc gagccgctga aagacctgat
     4681 cccgatcagc cgggtgagct cctcgccgct catcctcgcg gtgaatcccg gcaccggcat
     4741 cgactcggtc agggagctca tcgcccacgc gaagaagcat cccggcacgc tcaatttcgc
     4801 gacctcggga aacggctcgg cgccgcatct cgccgcggtg ctgttcatgc gccttgcggg
     4861 cgtcgagatc gtgcacgtgc cctacaaagg cggcgcgccc gcggtgcaat cggtgctcgc
     4921 cggcgatacc cagctcgcgt tcgccacgcc gccttcggtg ctgccgctgg tgcaggccgg
     4981 gcgcatgcgc gcgctggcga cgaccagccg cgccgcgacc cccctgatcc cgggcatacc
     5041 gggaatgggc gaagcaggtc tgcccgatta cgagatctcg ttctggtacg gcctattcgt
     5101 gccggagggc acgccggcgg cggtggtgcg caccttgttt gcggcgatca agcaggtggt
     5161 caaggcgccg gagaccgcgc gcatccttgc gcgcgagggc accgaggcgg tcggctcagg
     5221 ctcgcccgac gagttcgcga tcttcctcga gcaggatgcc aagctctggg cccgcctcgt
     5281 gaaggagtcg ggcgccaagg ccgagtgagc tggccttaga ggcggcggcc gaggatggtg
     5341 tccatgttgc cgccggtctt caggcaggcg gcgaacagat cctcggcgaa tttctggctg
     5401 gtcgccccgt tcgtgcggta ggcggcgtcg aggatatctt tcatgagctc tttgccgcgc
     5461 gacgattccg agacgtcgta gatgcgcgcc atgatggcgc cggcctgcgg gcgctgcact
     5521 tcctcttcgg ccagcgagcg cgcgaccacg gccatgtcgg ccgcgatccc gcattccacc
     5581 gcgctttcgg ctttcggggc ggccagcgcg ctgcctgcga cgagcgcgcc gaggagagct
     5641 gccaagagtc ttgtcatgag ggttattcct tcctgaaatc tcgttcgttt cgagtagcgc
     5701 cggcggcggc gccctgctgc accatcgcgt cgtattcgcc cagcgagagg cggctgtcgt
     5761 tgtccttgtc catctcgctg aaccggttgc tccatggggc gtcgcgcgct tcgtcgcgcg
     5821 agatgtagcc gtcgttgtta cggtccatcg ccgggaagcc acgcaccgag ggcgtggcgg
     5881 tattggtatc cctcggttcc tgaccgctcc ccgtctgctg cgcgagcacc ggcgttgtaa
     5941 agacggcggc cattgccagg gtcatgactg tcttaaggtt cataggacct ccatgaaggc
     6001 ggaccctcgc aatcctcgtg ccgtaatccg gtaggattgc cctccttcca tggaactagg
     6061 cattcaaggc cgtactgcgc tcgtcaccgg cgcaagcatg ggcatcggca ggggcatcgc
     6121 caccgcgctc gcgcgcgagg gagtcaagct cgccgtggtc gcacggcggc gcaacctgct
     6181 cgaggcgctc gaaagcgagc tcaagacaaa gctcgtgatc atcgagcagg actttctcaa
     6241 gcccggcgcg ccggaaagca ttgccgaaca agcattggcc ggcctcggct cgatcgacat
     6301 cctgatcaac aacgccggcg gcagccgcaa gttcacgctc gactcgagcg aagagcagtg
     6361 ggaggaggcg ctgacgctca acttcacgcg gcagcgccag ctcgcgcacc gcctgctgcc
     6421 gcagatgatc gcgaagaagt ggggccgcat cgtcaacatc accggcaagt ccgagccgga
     6481 agggttgaac ggcgcctttg cggccaaggc ggcgatgcat tcctgggcga agggactgtc
     6541 gcgcgaggtc ggaaagcacg gcatcacggt gaactgcatc ccgcccgggc gcatcatcag
     6601 cgagcagatc ctgcgcaact acccgcccga gtaccgcaag aagcagagcg aagaggagat
     6661 cccggtgggc gagtacggcc agccggccga catcgcgaac ctggtgtgct ttctcgcttc
     6721 gccgctcgcc cgatacatca ccggcgcggt gatccccgtc gatggcgggc tgcgccgtta
     6781 ccagttttaa ggggaaccga tgaggatttt tctgctagcg ctgtttttcg cttgtaccgc
     6841 tcacgggcaa acctacccga acaaacccat ccgcctggtc gttcccttcg cgcccggcgg
     6901 ctcgagcacc atcgtggcgc gcagcgttgc cgccgagatg gagaaggcgc tcgggcagtc
     6961 gatcgtgatc gagaacaagg gcggcggcgg cggcaacctg gcgatgcacg acgtggcgcg
     7021 cgccgacgcc gacggctaca cgctgatcat cggccacgtc ggcagcctgg cgatgacgcc
     7081 ctacatcacg cccaacctcg gctacgacgt gaacaaggac ttcgcgccgg tctcgctgct
     7141 cgccaaggtg ccgaacatct tcgtggtgca cgccgacgtg ccggcgaaga acctgcgcga
     7201 gttcgtcgcg ctggcgaaag cgaagcccgg gcagttctat tacggctcgg ccggcaacgg
     7261 cagcgccggg cacctggcga tggagtacct caagctcgtc agcggcatgg atctgcagca
     7321 cgtcccttac aaaggcaccg ggccgaacct cgtcgacctc gtcgccgggc gcacgcacgc
     7381 cacctcggcc ggcaccccgc cgctcatgcc gcacgtgaaa agcggcaagc tgcgcgtggt
     7441 cgcggtcggc accccgcagc gcctcgcctc gctgcccgac gtcgccaccg tggcggagca
     7501 gggctatccc ggtttcgaga ccagccagtg gtacggcttg aacgccccgg cgaagacccc
     7561 gccggcgatc atcaagcgcc tctcggacga ggcggcgaag gcggtgaaga gcgccggggt
     7621 catcgagcgc ttcaaggccg acgacgccga ggccgtgggc tcgagcccgc aggattacgc
     7681 gcagttcatt tcgaaagagc aggcgcgctg gaaggaagtg atccagaaag ccggcgtcaa
     7741 ggccgactga tggttgattt cgacgtgatc gttgtcggcg ggggcaacgc cgcgttgtgc
     7801 gcagcgctct ccgcttctga atctgcaaaa agagtactgg tcctggaaag agcgccggaa
     7861 gaggaagccg gcggcaactc gcgcttcacc gccggccttt tccgcctggt ctacaccggg
     7921 cccgaggatc tgaagacgct gatccccgac ctctcgcccg aggagatcgc caacaccgac
     7981 ttcggcacct acaccgaggg gcagtttctc gacgacatgg cgcggttgac cgagtacagg
     8041 tgcgacccgg atctgacgga aatattggta aaagaaagtt ttcccacggt gaagtggatg
     8101 cggcaaaaag gcgtgcgctt taccgccgcc tgggggcggc aggcgttcag gatcggcggc
     8161 aaattcaagt tctggggcgg cctcaccgtc gaggcggtgg gcggcggccc ggggttggtc
     8221 gaatccgaga cgcagctcgc gaagaaggcc ggcatcgaga tctggtacca ggcgcgcgcc
     8281 gttgcattga ttgccgacga cgatggcgtt catggcgtcg tggtcaagaa agcaggcaaa
     8341 acacttcaag caaaagccaa agcggtggtc ctcgccgcgg gcggcttcca ggccaacacc
     8401 gaatggcgca cgcgctacat gggcccgggc tgggagctcg ccaaagtgcg cggcacgcgc
     8461 ttcaacaccg gcgatgcgat cgagatggcg ctcgacatcg gcgccgcggc gcacggcaac
     8521 tggtcgggct gccacgcggt cgcctgggac cgcaacgcgc ctgagaccgg cgacctcgcg
     8581 gtgggcgacg gtttccagaa gcactcgtac ccctggggcg tgtacatcaa cgccgacggc
     8641 aggcgcttcg tcgacgaggg cgcggatttc cgcaactaca cctacgcgaa gtacggccgg
     8701 gtgatcctgc agcagccgaa gcagttcgcc tggcagatct tcgacgccaa ggtaaagcgg
     8761 cagctgcgcg acgagtaccg catcaagcag gtgacgaaga tcaccgccaa tactctcgag
     8821 gagctggtgt cgaagatgga cgacaccgat cagaaggccg cgctcgaaac gctgaagaaa
     8881 tacaacgcgg cggtgcgcac cgacatcccg ttcaacccca atgtgaagga cggtcgatgc
     8941 acggaaaatc taccggttaa taaatcgaat tgggccaata cgctggatac gccgccgttc
     9001 gaggcctacg cggtgacctg cggcatcacc ttcagctttg gcgggctaaa aataaattct
     9061 caaacacaag tcctgagctc ggacggcgag cccattgcgg gcctctacgc cgcgggcgag
     9121 ttggtgggcg gcatcttctg gttcaactac cccggcggct cgggactcac caatggtgcg
     9181 gtattcggac gaattgctgg cgcgaacgct gcgtcggcga gccgggagcg ataacatgga
     9241 acggaagatg actccggtcg cctcccgcgg catcaagttc gtcggccact cggacatggg
     9301 cttgcacggc gacggcgtgc aggtcatggt gcatcgcggc catgcctacg tcggccacgg
     9361 ctacagcaac ggcatcacca ccctcgatgt gcgcgatccg aagaacccaa aggtcaccga
     9421 cttcatcgcc tgcgcgccgg gcacacgcgc gtttcacctg cagacccacg acgatctgct
     9481 gctggcggtc aacgcgccga gcgtctggac catgcagcag ttccagaccg agaaggacta
     9541 tttcggcggc tcgcccgccg accggctgaa gggcaccggg gtccagttca gtgccggtat
     9601 ccgggtctac gacatttccc ggccggagaa gccgcgcgag atcggcttca tgccgaccga
     9661 cggcatgggt ccgcaccgca tctggtacgt gggcgggcgc tacgcgtacg cctccatcca
     9721 cttcgccgat ttcaccgacc acgtcctcgc cgtcatcgac atgtcggatc cgcgcaagcc
     9781 gcaggtcgtg ggccgctgct ggctaccggg catgtggcgc gccggcggcg aaacgccgac
     9841 cgcgcccgcc ggcaagcgct atgcgctgca ccacgcgctg ctcgacggcg acatcgcgta
     9901 cgccgcgtgg cgcgatggcg gcctgaccgt gatcaacgtc gccgatccgg ccaggccgaa
     9961 aatcctggtg caccgccttc cccaaccggg cggcggcggc acgcattcgc cgctgccgct
    10021 gccggaccga aagctcctcg tcctcgccga cgagccgact ttcgccaact gcaaggaagg
    10081 ccggcgccac atctggacct acgacgtgcg tgagccgtcc aatccggcgg tcatcgcgtc
    10141 cttcccggag cccggcgaag ccgactactg cgcaaagggc gggtttttcg gcccgcacaa
    10201 cctgcacgag aaccgtccgg gcgcattcca gagctcgcgc ctgatctttg ccacctacta
    10261 caacgccggt gtgcgggtct acgacatcgc ggaccaggcc cggccgaaag agatcgcgtt
    10321 ctacgtcccg cccaatccgg cgaagatggt ggatccgcgg ccgaaccggc cgcaggtgct
    10381 gcagtgcggc gactgctatg tcgatcgcaa cgggttcatg tacctgacgg accagaacgc
    10441 cgggcttaac attctgcagt tcgagggtct ctaacagagg aggggacgtg aaagcgaatt
    10501 ggctgggagc tgctctgata ctgggcgccg cattgggcac ggcgcacgcg cagcagacgg
    10561 tgttcattcc gatcgtgatc gaagtgtcgg gctcgggcgc ggtatcgggc accaacttcc
    10621 gcgacggcgc gctgatggcg atcgacgagg tcaacgcgaa gggcggcatc ctcgggcgca
    10681 ggatcaacgc accggtaagc gatacgcaga ccaacccggg catctcgcgc gcccaggtgc
    10741 agaaggcgct cgacggcaat ccctacgcga tcctcggccc ggtgttctcc ggctcggtga
    10801 aggtgaacat gctgctcgcc caggcggccg agatcccgca gttcaccggc gcggaggccg
    10861 ccgagatcac gcagatgggc aacgactaca tcttccgcac ctccttcggc cagcagatga
    10921 gcatgccgaa gatggcggtg ttcgtggccg acgggatcaa ggcgaagtcg gtggcgttcc
    10981 tgtgggtgaa caacgactac ggcaagggcg ggcgcgacgt gttcttcaag gagatggcga
    11041 agcgcaacat caagatcgtc gccgacgtct cgaccgaggc ggggcaggtg gacttcgccg
    11101 ccgacatcgt caagctcaag tcggccaacg cggacgcgat cttcatctac accaacgagg
    11161 aagagtgcgc gcgcgtgctg cgcgaggcgc gcaagcaggc cgtgaagacg ccgatcatcg
    11221 gcgagaccaa cgtcatcagc cagaaggtga tcgacctcgc gggcgacacg gccgagggcg
    11281 tccggggcca cgtcggactc accgccgatg cgccgatccc ggcggtgcag gcgttcacgg
    11341 aaaagttcca gaagcgctac aagtacgtgc cggaccacaa cggcatcaag ggctacaccg
    11401 cggtctacgc catcaagcac gccaccgaga agctgggcaa gttcgacagc aaggcgctcg
    11461 ccaagctgat gaagggcctg accatcacgc cgcagcagga gccgggcatc ctgatggagg
    11521 cgacctggga cgcgaagggc gacatcgacc gcatcagttt cctggtcgag gtggtgaagg
    11581 gtagacagaa agtcgtcgag gtgttgccca agctgggcaa gtagcctggc cgatttcctg
    11641 cagctcgcgg tcgcggggat cgcgaccggc ggtatctatg cgctggtcgc ggtcggcttc
    11701 gtgctgctgt ggcagacgtc gcagacgatc aacttcgcgc agggcgagtt cgtcatgctg
    11761 ccggcgttct tcgtgctcgc ggcgatgaaa ttcggcggcc tgtccttccc ggcggcgctg
    11821 ctggtcggcc tgctggtcgc gctcctcgtg ctggggctgc tcttcaagcg cctggtggtc
    11881 gacccgatcc tgcgccacgg cgtgctgccg ctcgtcatct ccacgctcgg cctcgccatc
    11941 ctcatgaagg agggcgtcaa ggagttctac gccgccgagg cgcaggcgtt cccgggcgtg
    12001 tggccgacga ccaacgtctc gatcggcggc gcggtcatct ccgcgcagag cctggtgatc
    12061 atcgccgtgg cagtcgtcgc agtcggactg ctgcagtact tcctgggcgc cacgcgcacc
    12121 ggccggcaga tgcaggccac cgcgcagaac ccgctgctcg cgcgcgtgct cggcattccg
    12181 gtcgagcgca tgatcctcta taccttcctg gtcaacgcgg cgctggtggc gctggcgtcg
    12241 ttcctggtga gcccgatcta cct
//
LOCUS       DATBSZ010000299         5727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_882772, whole genome shotgun sequence.
ACCESSION   DATBSZ010000299 DATBSZ010000000
VERSION     DATBSZ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882772"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..660
                     /locus_tag="VIG70_19735"
     CDS             133..660
                     /locus_tag="VIG70_19735"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY8252829.1"
                     /translation="MFRKITFWYSRLLSWLLVASVAVLIIPVSLQIFSRYTALIPSYI
                     WTEELARFCFIWSILLGAMVGIREGTHFVVDLWPAMNPRAQAAIRLVASVFVLVFALV
                     FLWWGIDFTRFAFNRISELAELPLWMIHIPWPIAGVTWVLFLGEQMWDDLRVMAGKAP
                     PASSRPPEEIPGSIT"
     gene            657..1973
                     /locus_tag="VIG70_19740"
     CDS             657..1973
                     /locus_tag="VIG70_19740"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY8252830.1"
                     /translation="MIGSTLAPGTASLIMFGVFFLLLVLRVPVAFALGLACVPIFLIE
                     ERLTPQNLVQETFNAYNSFILLAVPFFLLTANLMNVGGITTRLVRLSRDLVGHFPGGL
                     AQVNVVLSLFFAGISGSSTADAASQSKIFIEAQRKEGYDDSFSVAITAVSAVLAVIIP
                     PSILMIVWGGLLTVSIGGLFLAGIVPGVMIALAQMATVHVYAVRRGYPTYPRATLVET
                     FKAIGVAIPALMTPIIIIGGKIFGWFTATESACIAVLYAAALTMLVYREMDLKGLHHA
                     LLETGRLTGVALFCVGTASAFGWLLAYYQIPKALLEGVAAWGMGPVTTGFFIAGVFLV
                     VGCFLDAIPAIVIVGTVLEPLAKSVGMDPIHFAIVGIVALAFGLVTPPYGLCLMISCA
                     VAKIRILDAIKDVMIMLIPMFIVLALIIVFPQIPLFLPRLFPPGTL"
     gene            2027..2761
                     /locus_tag="VIG70_19745"
     CDS             2027..2761
                     /locus_tag="VIG70_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY8252831.1"
                     /translation="MIDGCARHGIRGISPWRDQLAQMGLREAKKSIREKNLVVTGLCR
                     GGFFTAKDWKDDNLRAIEEAHELQAQCLVLVVGGLLPASKDLLASRKRVRDSIAEVLP
                     VARKAGVPLAIEPLHPMQAAERACINTLEQALDLCDELGDGLGVAVDVYHVWWDPKLA
                     SQVKRAGKRILGYHICDWLSPTRDLLNDRGMMGDGVIDLPLIRSWVEAAGYRGFQEVE
                     IFSELDWWKRDPDDVLTTCKQRIAVS"
     gene            2778..4109
                     /locus_tag="VIG70_19750"
     CDS             2778..4109
                     /locus_tag="VIG70_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252832.1"
                     /translation="MNRREHLKGLFGLAAAGATGCSTAPSPSSATDPIPPQGIGHRIK
                     HISYSDQGGRPDAVQVMVNRRHVYVGHMFSNGITVLDANDPRNLKPVTYWSLGQGDFT
                     RTHQLQTANDLLLASNGANIVAMQSYDGQRGYFENNLADSITNRKKFRSGLSIHDISK
                     PGELREIAFLEIPGFGINRTFWTGGRYAYVSAHFDGFTDHILCIVDLNNITKPEIVSR
                     WWLPGMNRAAGEKPVLGPGRRSALHHMVVAGDRGYASWRDAGLTVHDVSDARNPKLLS
                     HINWSPPFPGGTHTAVPLPGRNLAVVCDEANAEKCAKGLFHTFVLDMRAPQNPVPVAT
                     LPTPRDRDYCAAPGTFGPHNLHENRPDSLQSESMIFATWNNAGVRIFDIKDQFAPKEV
                     AYWAPPAPKKMIDPRPKVTLAAKTADIYVMPDGLMYVTDWNAGLNVLQYEG"
     gene            4123..5145
                     /locus_tag="VIG70_19755"
     CDS             4123..5145
                     /locus_tag="VIG70_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY8252833.1"
                     /translation="MFLGNRKVEVRAFPDPTPGPGEVVIEMKASGMCGSDLKFYRSAP
                     GEAQKSLGFSADAEPFIGGHEPCGVVAARGPGVSEREAPIGQRVMDHHYSGCGVCKHC
                     RVGWSQLCREGITVYGVTANGGHAEYLKVPARTLVPLPDELSFVAGAAVSCGTGTAYG
                     ALRRMHLAGGSTLAVFGQGPVGISATLLGARMGARVIAVETNAERRALAKKFGAEVAV
                     ESADVIKELTKGEGVDLAMDTTGVSAARLAAVRSAKTWGTVCFVGEGGDVTLDVSRDM
                     LRKQLTIIGSWTFSAMGQLECARFAVDNGIDVDALFSHRWKLEQADEAYRVFDTQSTG
                     KGVIVF"
     gene            5174..>5727
                     /locus_tag="VIG70_19760"
     CDS             5174..>5727
                     /locus_tag="VIG70_19760"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY8252834.1"
                     /translation="MKKPVIALAIGDPAGISPELTAKVVALKEVREAARVVVVGDRRI
                     FDDGARIAGIQIADVEFQDLKHQNPSTIKRGVAAKAGGESALNNYRTALRMVADGKAD
                     AACFTPFNKQAMRMAEPTYDDEILFTSNVLGFKGLATEFNVLEGLWNARVTSHIPLAE
                     VSASLSIEKIVAALGLTATALRAAG"
BASE COUNT          946 a         1914 c         1848 g         1019 t
ORIGIN      
        1 aagaagctgg ccgacccggt gatggccgcg tacgccaagg aaatcggcgc cgaggacatc
       61 tacaacaaga tcaacaacct gaagtaatca acgctaaagg aacggcggcg cctggcgcgt
      121 cgccgttttt ttgtgtttcg aaaaatcacg ttttggtaca gccgcctgct gtcgtggctg
      181 ctggtcgcct cggtggcggt gctgatcatt ccggtatcgc tgcagatttt ttcgcgctat
      241 accgcgctga ttccgagcta catctggacc gaggagctgg cgcgcttctg cttcatctgg
      301 tcgatcctgc tcggcgccat ggtcggcatc cgcgagggca cgcacttcgt ggtcgacctg
      361 tggcccgcca tgaacccgcg tgcgcaggcc gcgatccggc tcgtggcgag cgtgttcgtg
      421 ctggtgttcg cgctcgtctt cctctggtgg ggcatcgact tcacgcgctt cgcgttcaac
      481 cgcatctcgg agctcgcgga gctgccgctg tggatgatcc acattccctg gccgatcgcc
      541 ggcgtcactt gggtgctatt cctcggcgag cagatgtggg atgacctgcg cgtcatggcg
      601 gggaaggcgc cgccagcctc atcccgacct cctgaagaaa tcccggggag catcacatga
      661 tcggctcgac gctcgccccg ggcaccgcca gcctgatcat gttcggcgtg tttttcctgc
      721 tcctcgtgtt gcgcgtgccg gtggctttcg cccttggact cgcctgcgtg ccgatcttcc
      781 tcatcgagga gcgcctcacg ccgcagaacc tggtgcagga gaccttcaac gcctacaact
      841 cgttcatcct gctggcggtg ccgttttttc tcctgaccgc aaacctgatg aacgtcggcg
      901 gcatcaccac gcgcctggtg cgcctgtcgc gcgacctggt cggccatttc cccggcggcc
      961 tcgcgcaggt gaacgtggtt ctgtcgctct tcttcgccgg catctcgggg tcgtccaccg
     1021 cggacgcggc gagccagtcg aagatcttca tcgaagcgca gaggaaggaa ggctacgacg
     1081 acagcttctc cgtcgcgatt accgccgttt cggcggtgct cgccgtgatc atcccgccgt
     1141 cgatcctgat gatcgtctgg ggcgggctgc tcacggtgtc gatcggaggc ctgtttctcg
     1201 ccggtatcgt gccgggcgtg atgatcgcgc tcgcgcagat ggcgaccgtg cacgtctacg
     1261 cggtgcgccg cggctacccg acctatccgc gcgccacgct ggtcgagacc ttcaaggcga
     1321 tcggtgtggc gattcccgcg ctgatgacgc cgatcatcat catcggcggc aagatcttcg
     1381 gctggttcac cgccaccgag tcggcgtgca tcgccgtgct ctatgccgcg gcgctcacca
     1441 tgctcgtcta ccgcgagatg gacctgaagg gactgcacca cgcgctgctc gagaccgggc
     1501 ggctcaccgg cgtggcgctt ttctgcgtcg gcacggcctc cgcgttcggc tggctgctgg
     1561 cctattacca gattcccaag gcgctgctcg aaggcgtcgc cgcctggggc atgggtccgg
     1621 tgacgaccgg tttcttcatc gccggcgtgt tcctggtggt cggctgcttc ctcgacgcga
     1681 ttcccgcgat cgtcatcgtc ggcacggtgc tcgagccgct cgccaagagc gtcggcatgg
     1741 acccgatcca cttcgccatc gtcggcatcg tcgcgctcgc tttcggcctg gtgacgccgc
     1801 cctacggcct gtgcctgatg atctcgtgtg cggtggccaa gatccgcatc ctcgacgcga
     1861 tcaaggatgt gatgatcatg ctgattccga tgttcatcgt gctcgcgctg atcatcgtct
     1921 tcccgcagat cccgctgttc ctgccgcgcc ttttcccgcc cggcacgctc tgatggaact
     1981 ctcactgaat acggcgaccg tccgtaaaca gtggaatctc gcgcaaatga tcgacggctg
     2041 cgcgcgccac gggatccgcg gcatctcgcc ctggagagat cagctggcgc agatgggcct
     2101 ccgggaagcg aagaaatcga ttcgagagaa aaacctcgtg gtcaccggcc tgtgccgcgg
     2161 cggctttttc acggcgaagg actggaaaga cgacaacctg cgcgccatcg aggaagcaca
     2221 cgagctgcag gcgcaatgcc tggtgctggt cgtggggggc ttgcttccgg cgtcgaagga
     2281 cctcctcgct tcacgcaaga gggtgcgcga ttccattgcc gaagttcttc cggtcgcacg
     2341 gaaagcgggc gttcccctgg cgatcgagcc gctgcatccg atgcaggcgg ccgagcgtgc
     2401 gtgcatcaat acgctcgagc aggcgctcga tctctgcgac gagctgggag acgggctcgg
     2461 cgtcgcggtc gacgtctatc acgtctggtg ggacccgaag ctcgcttccc aggtgaaaag
     2521 agccggcaag cggatcctcg gctatcacat ctgcgactgg ctctcgccga cccgcgatct
     2581 cctcaacgac cgcggcatga tgggggacgg cgtgatcgat ctgccgttga tcaggtcctg
     2641 ggtcgaggcc gccggctacc gcgggttcca ggaagtggag atcttctccg agctcgactg
     2701 gtggaagcgc gatcctgacg acgtgcttac gacttgcaag cagcgcatcg ccgtatcatg
     2761 acctcgagga ggggaccatg aatcgccgcg aacatctgaa gggtttgttc ggcctcgccg
     2821 ccgccggcgc gaccggatgc agcaccgcgc catccccatc gtcggccacc gacccgatcc
     2881 cgccgcaggg catcggccac cgcatcaagc acatctccta cagcgatcag ggcggccggc
     2941 cggacgcggt gcaggtgatg gtgaaccgcc gccacgtcta cgtcggccac atgttcagca
     3001 atggcatcac cgtgctcgac gcgaacgacc cccgcaatct gaagccggtg acctactgga
     3061 gcctagggca gggcgatttc acgcgcacgc accagctgca gaccgcgaac gacctgctgc
     3121 tcgcttccaa tggcgccaac atcgtcgcga tgcagtccta tgacggccag cgcggctact
     3181 tcgagaacaa tctcgccgac agcatcacca accgcaagaa attccgctcg ggcctgtcga
     3241 tccacgacat ctccaagccc ggtgagctgc gcgagatcgc gttcctcgag attccgggct
     3301 tcggcatcaa ccgcacgttc tggaccggcg ggcgctacgc ctacgtctcg gcgcatttcg
     3361 acggcttcac cgaccacatc ctgtgcatcg tcgatctcaa caacatcacc aagcccgaga
     3421 tcgtctcgcg ctggtggctg ccgggcatga accgcgcggc gggcgagaag ccggtgctcg
     3481 ggcccggccg gcgctcggcg ctgcaccaca tggtcgtcgc cggagaccgc ggctacgcct
     3541 cctggcgcga cgccggcctg accgtgcacg atgttagcga cgcgcgcaac ccgaagctgc
     3601 tgtcgcacat taactggtcg ccgccgttcc cgggcggaac acacaccgcg gtgccgctgc
     3661 ccggccgcaa cctggcggtg gtctgcgacg aagccaacgc ggagaaatgc gccaagggcc
     3721 tgttccacac gttcgtgctg gacatgcgcg cgccgcagaa cccggtcccg gtagcgacgc
     3781 tgccgacgcc gcgcgaccgc gactactgcg cggcgccggg cacgttcgga ccgcacaacc
     3841 tgcacgaaaa ccggccggac tccctgcaga gcgagagcat gatcttcgcc acctggaaca
     3901 acgccggcgt tcgcatcttc gacatcaagg accagttcgc gccgaaagag gtcgcctact
     3961 gggcgccgcc ggcgccgaag aagatgatcg acccccggcc gaaggtgacg ctcgcggcaa
     4021 agacggcgga catctacgtc atgcccgacg gcctgatgta cgtcaccgac tggaacgcag
     4081 gcctgaacgt cctgcaatac gagggctgat atgcgcggag ccgtgttcct cggtaaccgc
     4141 aaggtcgaag tacgcgcgtt ccccgacccc acgccgggtc cgggagaagt cgtcatcgaa
     4201 atgaaagcct cgggcatgtg cggctcggac ctcaagttct accgctcggc gccgggcgag
     4261 gcgcagaagt cgctcggctt cagcgccgac gccgagccgt tcatcggcgg gcacgagccc
     4321 tgcggggtgg tggcggcgcg cgggcctggc gtttcggagc gcgaggcgcc gatcggccag
     4381 cgcgtcatgg atcatcacta ttccggctgc ggtgtctgca agcactgccg cgtcggctgg
     4441 tcgcagctct gccgcgaggg aatcaccgtc tatggcgtca ccgccaacgg cggccatgcc
     4501 gagtacctca aagtgccggc gcgcacgctg gtgccgctac ccgacgagct ttccttcgtc
     4561 gcgggcgcgg cggtgtcgtg cggcacgggc accgcgtacg gcgcgctgcg gcgcatgcac
     4621 ctcgccggcg gctcgacgct cgcggtcttc ggccagggac cggtcgggat cagcgctacg
     4681 ctgctcggtg ccaggatggg tgcgcgcgtc attgcggtgg agaccaatgc cgagcgccgc
     4741 gcgctggcga agaagttcgg cgccgaagtg gcggtggaat cagccgacgt catcaaggag
     4801 ctcacaaagg gagagggtgt ggatctggcg atggatacca cgggcgtttc ggcggcgcgg
     4861 ctggccgccg tgcgcagcgc gaagacctgg ggcacggtgt gctttgtcgg cgagggcggc
     4921 gacgtcacgc tcgacgtcag ccgcgacatg ctgcgcaagc agctcaccat catcggctcg
     4981 tggaccttca gcgccatggg ccagctcgag tgcgcgcgct tcgcggtcga caacggtatc
     5041 gacgtcgacg cgctgttctc gcaccgctgg aagctcgagc aggccgacga ggcctaccgc
     5101 gtgttcgata cccagtcgac cggcaagggc gtcatcgttt tctgaaaagc gcgccccgct
     5161 tcgggaggtt gacttgaaga aacccgtaat cgcgcttgcc atcggcgacc cagccggcat
     5221 cagcccggag ctgacggcca aagtcgtggc cctgaaggaa gtgcgcgagg ctgcgcgcgt
     5281 cgtggtggtc ggcgaccggc gcatcttcga cgacggtgcc aggatcgccg ggattcaaat
     5341 cgctgatgtc gaatttcagg atctcaagca ccagaacccg tcgacgataa agcgcggcgt
     5401 ggcggcgaag gcgggcggcg agtccgccct caacaactat cgcacggcgc tgcgcatggt
     5461 cgccgacggc aaggccgacg cggcgtgctt cacgcccttt aacaagcagg cgatgcgcat
     5521 ggccgagccg acctacgacg acgagatcct tttcacctcc aacgtgctcg gcttcaaggg
     5581 actcgccacc gagttcaacg tgctcgaggg gctgtggaac gcgcgcgtca cctcgcacat
     5641 cccgctcgcc gaggtttcag ccagtctctc catcgagaag atcgtcgccg cgctcgggct
     5701 taccgccacg gcgctgcgcg cggccgg
//
LOCUS       DATBSZ010000300         5913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1497749, whole genome shotgun sequence.
ACCESSION   DATBSZ010000300 DATBSZ010000000
VERSION     DATBSZ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497749"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VIG70_19765"
     CDS             <1..624
                     /locus_tag="VIG70_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY8252835.1"
                     /translation="SRTLEGIRRGEGDILVGTQLLAKGHDFPALTLVGVLNADSALVS
                     TDYRAPERLFAVLAQVSGRAGRRERPGEVLIQTRYPRHPLFAALERHDFAGFAQAQLA
                     ERKSAGFPPFVFEAALRAEAPRLEAALEFLRSAAASVRPPDEVQVFDPVPHLLTRRAD
                     LERAQLVMQSSSRPALQEFLGQLSEKLFAEAPRAIRWHLDVDPIEFD"
     gene            637..2268
                     /gene="argS"
                     /locus_tag="VIG70_19770"
     CDS             637..2268
                     /gene="argS"
                     /locus_tag="VIG70_19770"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY8252836.1"
                     /translation="MTSDPKARLADAYRQALAKEAPEHADAPVVFEKPRQPEHGDFAC
                     SIALQLAKRLKRNPRQLAESLTAAAVAAYPQFKSDFLGYEIAGPGFINFRLNPALKLA
                     AIRLALQPGWGFVRKAKPDSVQVEFVSANPTGPLHVGHGRQAALGDALASLIEAQGDK
                     VTREFYYNDAGAQIHNLALSVQARARGVKPGEPGWPSDGYAGEYIAEIAKAYAGPEDL
                     DSVRRFAVDYLRKEQDIDLREFGVKFDVYYLESSLYSDGKVDEVVKALTASGKTYEKD
                     GALWLRTTDYGDDKDRVIRKSDGTYTYFVPDVAYHLTKWRRGFTKVINVQGTDHLGTT
                     ARVRAGLQALKVGVPQGYPDYVMHSMVRVMRGGEEVKLSKRAGSYVTLRDLLDWVGRD
                     AVRFFLLSRRADADFVFDVDLARAQTEENPVYYVQYAHARVCSVIGQWRGDASRLAQA
                     DLGALTHERELALAQRLGEFPDVVAEAARELSPHSVTFYLRSLAAEFHSYYNAERILV
                     DDEALRTARLALCAAVRQTLASGLSLIGVGAPEKM"
     gene            2290..2874
                     /locus_tag="VIG70_19775"
     CDS             2290..2874
                     /locus_tag="VIG70_19775"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY8252837.1"
                     /translation="MTRRPRREQGNFLAGIGVGVLIGLAVSLGIAFYLNRTPIPFMTA
                     KPKQAEKNGAAAKPPAISGLPQSGVGPAATAEKPKFDFYRILPGSEEPVSEREIRDRL
                     KAKGGGQQEAAKDVYFIQAGSFQNPADADNQKARLAILGFESSVEPANLPDKGTWYRV
                     RMGPYAKLDEINKVRQVLAQSGIEASLIKIKDQQ"
     gene            2871..3476
                     /locus_tag="VIG70_19780"
     CDS             2871..3476
                     /locus_tag="VIG70_19780"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY8252838.1"
                     /translation="MNAVRRSLIAVIGLIPFMAQAQRGGQFMELNPPQPVETANKIEV
                     LEFFWYGCIHCYNLEPKIDTWLKTLPKDVEFRRVPAVFNERWAHDAAIFYTFEAMGLL
                     DKLHRPFFDAIHRDRLRTDNMQALNAWLEKQGVDPKKFEATVKSFGVQSKTKRAIRMT
                     SGYQIDGTPAMAVHGRYTVGASEGMLEIVNNLVAAVRKNKP"
     gene            3473..4231
                     /locus_tag="VIG70_19785"
     CDS             3473..4231
                     /locus_tag="VIG70_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8252839.1"
                     /translation="MARVRIVITGASSGIGDALVRQYAADGVVLGLISRRKAEGKGLP
                     GQIASYAVDVTDERALAAAAEDFIRRFGAPDLVIANAGIASGTEGADLRDVAKLRKVL
                     EVNVAGLAATLAAFAPAMRAAGRGTLAGIASVAGFRGLAGNGAYCASKSAAITWMESL
                     RAELHGSGVSVVCICPGYIDTPMTQVNRFRMPFLLSADEAARRIARAIARKRRLAVIP
                     WQMALVSIALRAMPGWLYDRIASRAPRKPRDVPV"
     gene            4228..4701
                     /locus_tag="VIG70_19790"
     CDS             4228..4701
                     /locus_tag="VIG70_19790"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEY8252840.1"
                     /translation="MRKFFLLVLLAASPTAAEDSNLLSYPSGYRQWTHVKSMHIRPGH
                     PLHHLFGGVHHIYANKQAREGYRRGKFGNGATLVLDLLDARDDGFAVTEGPRRMVAVM
                     HKDAKKYPQTGGWGFEAFRADNRKDRMVGTNAAAQCFECHAQRKKNDFVFSSYRP"
     gene            complement(4685..>5913)
                     /gene="phaC"
                     /locus_tag="VIG70_19795"
     CDS             complement(4685..>5913)
                     /gene="phaC"
                     /locus_tag="VIG70_19795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEY8252841.1"
                     /translation="LLADIEKGGGKLRISMTDESAFQLGRNVATTPGKVVFQNDLMQL
                     IQYAPLTEQVYKRPLVIIPPWINKYYILDLREKNSFIRWAVQQGHTVFVISWVNPDAS
                     LAQKGFEDYMLEGPLAAMEAIEKGTGAREMNVIGYCLGGTLLGAALGYLTHKKQQNRV
                     ASATFFVSLLDFSQPGELGVFIDEEQVASLEKMNERGYLEGSEMAGTFNLLRANDLVW
                     SFVVNNYLMGKDPFPFDLLYWNADSTRMPARMHSYYLRNMYIKNLMGVPGGLELAGVP
                     IDLSQVTLPAYFISTVEDHIAPWKTTYRGARYLGGPVRFVLGGSGHIAGIVNPPAAKK
                     YHYWTNKTLAESPDEWFSNATQHPGSWWEDWQRWIEPHGGGKAPARIPGEGALRAIED
                     APGSYARLRIASKAGS"
BASE COUNT         1015 a         1979 c         1935 g          984 t
ORIGIN      
        1 tcgcgcaccc tggaaggcat ccgccgcggc gaaggcgaca tcctggtcgg cacccagctc
       61 ctcgccaagg gccacgattt ccccgcgctc acgctggtcg gggtcctgaa cgcggacagc
      121 gcgctcgttt cgaccgacta tcgcgcgccc gagcggctgt tcgcagtgct ggcgcaagtg
      181 tcggggcgcg ccggccggcg cgagcgcccg ggcgaagtgc tgatccagac gcgctatccg
      241 cgccacccgc tgttcgccgc gctcgagcgg cacgacttcg ccggcttcgc gcaagcccaa
      301 ttggcggaac gcaagtcggc aggattcccg ccgttcgtgt tcgaggcggc gcttcgcgcc
      361 gaggccccgc gccttgaagc ggcgctggag ttcttgcgaa gcgctgcggc ctcggtgcgc
      421 ccgcccgatg aagtgcaggt gttcgacccg gtgccgcacc tcctcacccg ccgcgccgac
      481 ctcgagcgcg cgcagctcgt catgcagtcg agctcgcggc ctgcgctgca ggagtttctg
      541 gggcagcttt ccgaaaagct cttcgccgaa gcaccgcgcg ccattcgctg gcacctcgac
      601 gtcgacccca ttgaattcga ctgaatataa tcttttgtga cttctgaccc gaaagcgcgc
      661 cttgccgacg cctaccgcca ggcgctcgcc aaggaagcgc cggagcacgc cgacgctccc
      721 gtggtgttcg agaagccgcg gcagcccgag cacggcgact tcgcctgctc gatcgcgctg
      781 cagctcgcca agcggctgaa acgcaacccg cgccagctgg cggagtccct caccgcggcg
      841 gcggtggccg cgtatccaca gttcaaaagc gattttctgg gttacgagat cgccggtccc
      901 ggcttcatca atttccggct gaaccccgcg ctcaagctcg ccgcgatccg cctcgctctt
      961 caacccggct ggggattcgt ccgcaaggca aagcccgact cggtgcaggt cgagttcgtc
     1021 tccgccaatc ccaccgggcc gcttcacgtc ggccacggca ggcaggcggc gctcggcgac
     1081 gcgctggctt cgctcatcga agcgcagggc gacaaggtaa cgcgcgagtt ctactacaac
     1141 gacgcgggcg cgcagatcca caacctcgcg ctctccgtgc aggctcgggc gcgcggcgta
     1201 aagcccgggg aacctggctg gccgtccgac ggatatgcgg gcgaatacat cgcggagatt
     1261 gccaaggcct acgcagggcc cgaagacctg gattccgtgc gccgctttgc cgtggattat
     1321 ttgagaaaag aacaggacat cgacctgcgc gagttcggcg tcaagttcga cgtctactac
     1381 ctggaatcga gcctctacag cgacggcaaa gtggacgagg tcgtgaaggc gttgaccgcc
     1441 agcggcaaga cctacgagaa ggacggcgcg ctgtggctgc gcaccaccga ctacggcgac
     1501 gacaaggacc gcgtgatccg caagtcggac ggcacctata cctatttcgt gcccgacgtg
     1561 gcctaccacc tcaccaagtg gcggcgcgga ttcaccaagg tgatcaacgt gcagggcacc
     1621 gaccacctgg ggaccaccgc gcgggtgcgc gccggcctgc aggcgctgaa agtcggcgtg
     1681 ccgcagggct atcccgacta cgtgatgcac agcatggtgc gggtgatgcg cggcggcgag
     1741 gaggtgaagc tctccaagcg cgccggctca tacgtgacgc tgcgcgacct cctcgactgg
     1801 gtcgggcgcg acgcggtgcg cttcttcctg ctttcgcgcc gcgccgacgc cgacttcgtg
     1861 ttcgacgtcg acctcgcgcg cgcgcagacc gaggaaaacc cggtctacta cgtgcagtac
     1921 gcgcacgctc gcgtctgcag cgtaattggc cagtggaggg gcgatgcgag ccgtctcgcg
     1981 caggcggacc tgggcgcgct gacccacgaa cgggagctcg cactggcgca gcggctcggc
     2041 gagtttcccg acgtcgtggc cgaagccgcg cgcgagctct cgccgcactc ggtcaccttt
     2101 tacctgcgca gcctcgcggc cgagtttcac agctactata atgccgagcg catcctcgtc
     2161 gacgacgagg cgctgcgcac ggcgcggctc gcgctgtgcg cggcggtacg ccagacgctg
     2221 gccagcgggc tgtcgctcat tggcgtcggc gctccggaga agatgtaaat gcggcgcatg
     2281 ccccgatcga tgaccaggcg acccagacgc gagcaaggca acttcctcgc cggcatcggc
     2341 gtcggcgtgc tgatcgggct cgccgtttcc ctcggcatcg cgttctatct caaccgcacc
     2401 ccgatcccgt tcatgacggc gaagcccaag caggccgaga agaacggcgc cgcggccaag
     2461 ccgccggcga tctccggcct gccgcagagc ggcgtggggc cggcagccac ggcggagaag
     2521 ccgaaattcg acttctaccg catcctgccg gggtcggaag agccggtgag cgagagggag
     2581 atccgcgatc gcctcaaggc caagggcggc ggtcaacagg aagcggccaa ggacgtttat
     2641 ttcatccagg caggctcgtt ccagaacccc gccgacgccg acaaccagaa ggcgcggctc
     2701 gcgatcctcg gcttcgagtc gagcgtcgag ccggcgaacc tccccgacaa ggggacctgg
     2761 tatcgcgtgc ggatggggcc ctacgccaag ctcgacgaaa tcaacaaggt gcgtcaggtg
     2821 ctggcacaga gcgggatcga ggccagcctg atcaagataa aggatcagca atgaacgcgg
     2881 tacgccgcag tctcatcgcc gtcatcggtc ttattccgtt catggcgcaa gcccagcgcg
     2941 gcggccagtt catggagctc aatccgccgc agccggtgga gacggcaaac aagattgaag
     3001 tgctggaatt cttttggtac gggtgcattc actgctacaa cctcgagccg aagatcgaca
     3061 catggctgaa gacgcttccc aaggacgtcg agttccggcg cgtcccggcc gtgttcaacg
     3121 agcgctgggc gcacgatgcg gcgattttct acaccttcga agcgatgggg ctgctcgaca
     3181 agttgcaccg gccgttcttc gatgccatcc atcgcgaccg cctgcgtacc gacaacatgc
     3241 aggcgctgaa cgcgtggctg gagaagcagg gcgtcgaccc caagaaattc gaggccacgg
     3301 tgaagtcgtt cggcgtgcag agcaagacca agcgcgcgat ccgcatgacc tctggctacc
     3361 agatcgacgg cacgccggcc atggcggtcc acggacgcta caccgtgggc gcgagcgaag
     3421 ggatgctcga gatcgtcaat aacctggtcg ctgcagtccg caagaacaag ccatagcacg
     3481 ggtgcgtatc gtcatcaccg gcgcttcctc cggtatcgga gatgcgctgg tgcggcagta
     3541 cgccgccgat ggcgtcgtcc tcggcctcat ttcccgccgc aaagccgaag gcaaggggct
     3601 tcccgggcaa atcgcttcct acgcggtcga cgtcaccgat gagcgggcgc tggccgcggc
     3661 cgccgaggat ttcatccgcc gcttcggcgc tcccgatctc gtgatcgcaa acgccggcat
     3721 cgcgagcggc accgagggcg cagacctgcg ggatgtcgca aagctcagga aggtgctgga
     3781 ggtgaatgtc gccggccttg ccgcgaccct cgctgccttt gcgccggcga tgcgcgcggc
     3841 cgggcgcggc acgcttgccg gcattgcgag cgtggcgggc ttccgcggcc tcgccggcaa
     3901 cggtgcctat tgcgcgtcga aatcggcggc gatcacctgg atggaaagcc tgcgcgccga
     3961 gctccatggc agcggcgtgt cggttgtctg catctgcccg gggtacatcg acaccccgat
     4021 gacgcaggtc aaccgctttc gcatgccttt cctgctgtcc gccgacgagg cggcgcgccg
     4081 cattgcgcgc gccatcgcga gaaagcggcg gctcgcggtg attccctggc agatggcgct
     4141 ggtttcgatc gcgctgcgcg ccatgccggg ctggctctac gaccggatcg cttcgcgcgc
     4201 cccgcgcaag ccccgcgacg ttcccgtatg aggaagttct ttctcctggt tttgctggcg
     4261 gcgagcccca cggcggccga ggactcgaat ctgctcagct atccgagcgg ctaccggcaa
     4321 tggacccacg tcaagagcat gcacatccgc ccggggcatc cgctgcacca tctcttcggc
     4381 ggcgtgcacc acatctacgc caacaagcag gcccgcgaag gttacaggcg cggcaagttc
     4441 ggcaacggcg cgacgctggt gctggacctg ctggacgcgc gcgatgacgg cttcgctgtc
     4501 accgaggggc cgcgcaggat ggtggcggtg atgcacaagg acgccaagaa gtacccgcag
     4561 accgggggct ggggcttcga agcgttccgc gccgacaatc gcaaggaccg gatggtgggc
     4621 acgaacgccg cggcgcagtg cttcgaatgc cacgcgcagc ggaaaaagaa cgacttcgtc
     4681 ttttctagct accggcctta gacgcaatcc gcagcctggc gtagctgccg ggcgcgtctt
     4741 cgatcgcgcg cagggcgccc tcgccgggga tgcgcgccgg cgccttgccg ccgccgtgcg
     4801 gctcgatcca gcgctgccag tcttcccacc acgagccggg atgctgcgtg gcattgctga
     4861 accattcgtc gggcgactcc gcgagcgtct tgttggtcca gtagtggtat ttcttcgccg
     4921 ccggcgggtt gacgatgccg gcgatatggc ccgagccgcc cagcacaaac cttaccggcc
     4981 cccccaggta ccgtgcgccg cgatacgtcg ttttccacgg cgcgatgtgg tcctcgacgg
     5041 tcgagatgaa gtaggcgggg agcgtcacct gggagaggtc gatcggcacg ccggcgagct
     5101 cgaggccgcc cggcacgccc atcaggttct tgatgtacat gttgcgcagg tagtagctgt
     5161 gcatgcgcgc cggcatgcgc gtcgagtcgg cgttccagta cagcagatcg aacggaaacg
     5221 ggtccttgcc catcaggtag ttgttcacca cgaacgacca caccaggtcg ttcgcgcgca
     5281 acaggttgaa ggtgcccgcc atctccgagc cctcgaggta gccgcgctcg ttcatcttct
     5341 cgaggctcgc cacctgctcc tcgtcgatga acacgccgag ctcgccgggc tgcgagaagt
     5401 cgagcaggct gacgaagaag gtcgcgcttg ccacgcggtt ttgttgtttt ttatgagtta
     5461 aataccccaa ggctgctcca aggagcgtgc cacccaggca atagccgatg acgttcatct
     5521 cgcgcgcgcc ggtgcctttc tcgatcgctt ccatcgcggc gagcggccct tcgagcatgt
     5581 agtcctcgaa gcccttctgc gccaggctcg cgtctggatt gacccaggag atgacgaaca
     5641 ccgtgtgccc ctgctgcacc gcccagcgga tgaaggagtt cttctcccgc aggtcgagga
     5701 tgtagtactt gttgatccac ggcgggatga tgaccagcgg ccgcttgtac acctgctcgg
     5761 tgagcggggc gtactggatc agctgcatca ggtcgttctg gaacaccacc ttgcccggcg
     5821 tggtggcgac gttacgcccg agctggaagg cgctctcgtc ggtcatcgag atgcgcagct
     5881 tgccgccgcc cttctcgatg tcggcgagca ggt
//
LOCUS       DATBSZ010000301         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_199163, whole genome shotgun sequence.
ACCESSION   DATBSZ010000301 DATBSZ010000000
VERSION     DATBSZ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199163"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VIG70_19800"
     CDS             <1..914
                     /locus_tag="VIG70_19800"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HEY8252842.1"
                     /translation="ARKKRLPFAIEIIGFGDEEGVRFGTTLLGSRAVAGIFDGKALHA
                     QDASGTSMSKALSDFGLDPKRIPEVARKKSDVLAYAELHIEQGPVLEAEGLPVGVVTA
                     INGFSRLRVTLRGVAGHAGTVPMNLRRDALAGAAECVLAAEKIAKGHPELVATVGRIE
                     AKPGAINVIPGEVVFTVDVRAPQDPLRKHAVAAIRSQIEQVSKNRKLDCEIETLQEFG
                     VSTCAPWLMEQMDQAVARQGFRVRRLPSGAGHDGMALGAITDICMLFVRCKGGISHSP
                     LESITEADAAAGARVLLDFIEHFQLPA"
     gene            889..1800
                     /gene="puuE"
                     /locus_tag="VIG70_19805"
     CDS             889..1800
                     /gene="puuE"
                     /locus_tag="VIG70_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HEY8252843.1"
                     /translation="MSTSSYPRDLVGYGPRPPKPKWPGSARVALQFVLNYEEGAENSV
                     LHGDKASEAFLSEIVGAQPLEGVRHMSMESIYEYGSRAGVWRLLSLFARYELPLTVFG
                     VAMAMERNPSAVDAFLKAKHEVASHGWRWINYQYVPLEVEREHMQRAIEIHRRLTGER
                     PLGWYTGRTSPNTRRLVVEDGGFVYDADDYNDDLPWYDTRHRDGSGKPQLVVPYTLDA
                     NDMRFATAQGFNSGDDFFNYLKDSFDVLYAEEGRMMSVGLHCRLVGRPGRAAALEKFI
                     RYVRGKKVWVARRIDIARHWLKNHPHG"
     gene            1793..>2115
                     /locus_tag="VIG70_19810"
     CDS             1793..>2115
                     /locus_tag="VIG70_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252844.1"
                     /translation="MAERLELFEDLNPPQRLLMGPGPINAHPRVLQAMSAPLLGQFDP
                     RFREYMKQTSALYRGVFQTANRWALLVDGTARAAIEAALVSLIEPGDRVLVPVFGRFG
                     HLKVE"
BASE COUNT          370 a          701 c          715 g          329 t
ORIGIN      
        1 atgcgagaaa aaagcgcctg ccgttcgcca tcgagatcat cggcttcggc gacgaggaag
       61 gcgtgcgctt cggcaccacg ctcctcggca gccgcgcggt cgcggggatt ttcgacggca
      121 aggctttgca cgctcaagac gcgagcggca caagcatgtc caaagcccta agcgacttcg
      181 ggctggatcc gaaacgcatc cccgaggtgg cgagaaagaa aagcgacgtg ctcgcctacg
      241 cggagctcca catcgagcag gggccggtgc tcgaggccga gggcttgccg gtcggcgtgg
      301 tgacggcgat caacggcttc tcccgcctgc gcgtgacgct gcgcggcgtg gccgggcacg
      361 caggcaccgt gccgatgaat ctgcggcgcg acgcgctcgc cggcgcagcg gaatgcgttc
      421 tggcagcgga aaagattgcc aaagggcatc cggagctggt cgccacggtc ggccgcatcg
      481 aggcgaagcc cggggcgatc aacgtaattc cgggcgaagt cgtcttcacc gttgacgtcc
      541 gcgcgccaca ggaccccctc aggaagcatg cggtcgctgc cattcgttcg cagattgaac
      601 aggtttcgaa aaaccggaag ctcgactgcg aaatcgaaac gcttcaggaa ttcggcgtct
      661 ccacctgcgc gccgtggctc atggagcaga tggaccaggc cgtggcgcgc caggggttcc
      721 gcgtgcgccg cctgccctcc ggcgccggcc acgacggcat ggcgctcggc gcgatcaccg
      781 acatctgcat gctgttcgtg cgctgcaagg gcggcatcag ccatagtccc ctggaatcca
      841 ttaccgaagc agacgcggcc gccggcgcgc gcgtgctgct cgatttcatt gagcacttcc
      901 agctacccgc gtgacctcgt gggctacggc cccaggccgc cgaagccgaa atggcccggc
      961 agcgcgcgcg tcgcgctgca gttcgtcctc aactacgagg aaggcgccga gaactcggtc
     1021 ctgcacggcg acaaggcttc ggaggcgttc ctctccgaga tcgtgggcgc gcagccgctc
     1081 gagggcgtgc gccacatgtc gatggagtcg atctacgagt acggctcgcg cgcgggcgtg
     1141 tggcggctgc tctcgctttt cgcgcgctac gagctgccgc tcaccgtgtt cggcgtggcg
     1201 atggcgatgg agcgcaatcc ttcggcggtc gacgcgttcc tcaaggcgaa gcacgaggtc
     1261 gcgagccacg gctggcgctg gatcaactac caatacgtgc ccctcgaggt cgagcgagag
     1321 cacatgcagc gcgcgatcga aatccaccgc cggctcaccg gcgagcgccc gctcggctgg
     1381 tacaccggcc gcaccagccc gaatacccgc cgcctggtgg tcgaggacgg cggcttcgtc
     1441 tacgacgccg acgactacaa cgacgacctg ccgtggtacg acacgcgaca cagggacggg
     1501 agcggcaagc cgcagctcgt cgttccctat acgctggacg cgaacgacat gcgcttcgcc
     1561 acggcgcagg gattcaactc gggggacgat tttttcaact acctgaagga cagcttcgac
     1621 gtgctgtacg ctgaagaggg ccgcatgatg tcagtcggcc tgcactgccg gctggtcggc
     1681 aggcccggac gagccgccgc gctggaaaaa ttcatccgct acgtcagagg caagaaggtg
     1741 tgggtcgccc gccgcatcga catcgcccgc cactggctga agaaccatcc tcatggctga
     1801 gcggttggag ttgtttgagg acctgaaccc gccgcagcgc ctgctcatgg gcccgggccc
     1861 gatcaacgcc cacccccgcg tgctgcaagc gatgagcgcg ccgctgctcg ggcagttcga
     1921 cccgcggttc cgcgagtaca tgaagcagac ctcggcgctg taccgcgggg tgttccagac
     1981 cgcgaaccgc tgggcgctcc tcgtcgacgg cacggcgcgc gcggcgatcg aagcggcgct
     2041 ggtgtcgctg atcgagccgg gagaccgggt gctggtgccg gtgttcggcc gcttcggcca
     2101 cctcaaggtc gagat
//
LOCUS       DATBSZ010000302         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_539936, whole genome shotgun sequence.
ACCESSION   DATBSZ010000302 DATBSZ010000000
VERSION     DATBSZ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539936"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="VIG70_19815"
     CDS             <1..1220
                     /locus_tag="VIG70_19815"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY8252845.1"
                     /translation="PPADQALAYEILPFAEMPQLVNPARGYIVNGNNDPIGVTLDNNP
                     LNKLRAGGQGILYLSPGYAIGNRAARIDRRIRAELARPDRHGGDLSFEDMMRIQADVK
                     LLDAEVLTPYLLRAFDAARAAGAPAPLAALGADPAVAEAVARLRRWDFSTPTGIAEGY
                     DAADRHGKRSKPTREEIANSVAATIYSVWRGQALRNVIDGTLARVGLTIVPPGDRAMI
                     ALRHLLEAFPASGSGLSFFDVPGMTLAPAAERDLVLLQSLKGALDLLASPAFASAFGQ
                     STDQDDYRWGKLHRIVLAHPLGAPFSIGPLAIDGGFDVVDASSHNPRAATPEAFMFSS
                     GPSKRFVGEARRSGIQAVQVIPGGASGVPGTPFFGSLVELWATNDYHEALSDLGDVLR
                     NAVSVQVFEPANH"
     gene            complement(1217..1621)
                     /locus_tag="VIG70_19820"
     CDS             complement(1217..1621)
                     /locus_tag="VIG70_19820"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEY8252846.1"
                     /translation="MTSTAEEDRPVILFDGVCNLCTGSVQFVIERDSRKQFRFASLQS
                     PVAEKLLGRRDDLESMVLVQGGTVYRKSTAALLIARRLDGLWPALSVFLVVPRFLRDA
                     VYDWIGRRRYRMFGKRDVCWTPRPELAERFLD"
     gene            complement(1605..2126)
                     /locus_tag="VIG70_19825"
     CDS             complement(1605..2126)
                     /locus_tag="VIG70_19825"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEY8252847.1"
                     /translation="MAELTFRQKLVVGFIAAWIGLQILVPLRYFFYPGNVSWTEEGHR
                     FSWMMKLRDKDAEARFTVRDPATGRQWRVSNERFLKRHQIGEMESRPDMILQFAHHLA
                     RVWEKERGISGVEVRARVCASLNGRKAELLIDPERDLARIERNLRHADWILPQKEPFE
                     RPDPRTGRVDLHC"
     gene            complement(2119..>2907)
                     /locus_tag="VIG70_19830"
     CDS             complement(2119..>2907)
                     /locus_tag="VIG70_19830"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEY8252848.1"
                     /translation="ALRVMFGVIMLVECWRFWSKGWIERHYIAPEFFFKYYGFEWVQA
                     WPGDGMYWHFAALAVLALLITLGVFYRAATVLFFLAFTYIFLLDQARYLNHFYLVILI
                     SVALMVIPAPHLRSPTPAWAIWLFRLQFEVMYIYAGIVKVNADWLRLEPMGMWLARRD
                     DFLLLGDLFQQDWVVAVASYGAIALHIIGAPLLLWKVTRGWVMVIYFAFHLMNHFMFN
                     IGIFPWVAMAGTLIFLEPDWPRRAYRATKQFAFKIFRIRAPAHG"
BASE COUNT          514 a         1004 c          969 g          420 t
ORIGIN      
        1 atccgcccgc ggaccaggcg ctcgcctacg agatcctgcc gttcgcggag atgccgcagc
       61 tcgtgaaccc cgcgcgcggc tacatcgtca acggcaacaa cgacccgatc ggggtcaccc
      121 tggacaacaa cccgctcaac aagctgcgcg cgggcgggca gggaatcctg tacctctcgc
      181 ccggttacgc catcggcaac cgcgcggcgc gcatcgaccg gcggatccgc gccgagctcg
      241 cgcgcccgga ccggcacggc ggcgatctct cgttcgaaga catgatgcgc atccaggccg
      301 acgtgaagct gctcgatgcc gaggtgctca cgccctatct gctgcgcgcc ttcgacgcgg
      361 cgcgcgctgc gggcgcgccg gcgccgctcg cggcattggg cgccgatcct gcggttgcgg
      421 aagcggtggc gcgcctgcgc cgctgggact tcagcacgcc gaccgggatc gcagagggct
      481 acgacgccgc cgatcgccac ggcaagcgat cgaagcccac gcgcgaagag atcgcgaaca
      541 gcgtggcggc gacgatctac tcggtgtggc gcgggcaggc gctgcggaac gtgatcgacg
      601 gcacgctcgc ccgtgtgggc ctgaccatcg tgccgcccgg agaccgggcg atgatcgcgt
      661 tgcgccacct gctcgaggct tttcccgcca gcggatcggg tctgagcttc ttcgacgtgc
      721 cgggcatgac tctcgcgccc gcggccgagc gcgacctcgt gctgctgcag agcctcaaag
      781 gcgcgctcga cctgctcgct tcacccgcgt tcgcgtccgc cttcggccag tcgaccgacc
      841 aggacgacta ccgctggggc aagctgcacc gcatcgtcct cgcccatccg ctcggcgcgc
      901 cgttcagcat cgggccgctc gcgatcgacg gcggtttcga cgtggtcgac gcctcgtcgc
      961 acaacccgcg cgccgcgacc cccgaagcgt tcatgttctc gagcgggccc tcgaagcgct
     1021 tcgtcggcga ggcgcggcgc tcgggaatcc aggcggtgca ggtgattccc gggggcgcga
     1081 gcggagtgcc ggggacgccg ttcttcggca gcctggtcga gctctgggcg acgaacgact
     1141 accacgaagc actgtccgac ctcggcgacg ttctgcggaa cgccgtgtca gtccaggtgt
     1201 tcgagccggc gaaccactag tccagaaagc gctccgcgag ctcgggacgc ggcgtccagc
     1261 agacgtcgcg cttgccgaac atgcggtagc gccgccggcc gatccagtcg tacaccgcgt
     1321 cgcgcaggaa ccgcggcacc acgaggaaaa ccgagagcgc cggccagagc ccatcgaggc
     1381 gcctggcaat gagaagcgct gccgtcgatt tgcggtagac cgtgccgccc tggaccagca
     1441 ccatcgactc gaggtcgtcg cggcgcccga ggagcttttc cgccaccggc gattgcagcg
     1501 aggcgaagcg gaactgcttg cgcgaatcgc gctcgatcac gaactgcacg ctgcccgtgc
     1561 agaggttgca cacgccgtcg aacaggatga cggggcggtc ctcttcagca gtggaggtca
     1621 acgcggccgg tcctcggatc cgggcgctcg aacggctcct tctggggcag gatccagtcg
     1681 gcgtggcgca ggttgcgctc tatccgggcg aggtcgcgct ccgggtcgat gagcagctcc
     1741 gccttgcggc cgttcagcga cgcgcacacg cgcgcgcgca cctcgacgcc cgaaatgccg
     1801 cgctccttct cccacacgcg cgcgaggtgg tgcgcgaact gcaggatcat atccggccgc
     1861 gactccatct cgccgatctg gtggcgcttg agaaaacgct cgttcgacac gcgccactgg
     1921 cgcccggtgg ccgggtcgcg caccgtgaag cgcgcctcgg cgtccttgtc gcgcagcttc
     1981 atcatccagg aaaagcggtg cccttcctcg gtccagctca cgttgccggg atagaagaag
     2041 tagcgcagcg gcaccaggat ctgcaggccg atccaggccg cgatgaagcc gaccaccagc
     2101 ttctggcgga aagtgagctc agccatgcgc aggcgcccgt attctgaata tcttgaacgc
     2161 gaactgcttc gtcgcgcggt acgcgcgccg cggccagtcg ggctcgagga agatcagcgt
     2221 tcccgccatc gccacccacg ggaagatgcc gatgttgaac atgaagtggt tcatcaggtg
     2281 gaaggcgaaa tagatcacca tcacccagcc gcgcgtaacc ttccagagga gcagcggcgc
     2341 gccgatgatg tggagcgcga tcgcgccgta cgaggcgacc gcgaccaccc agtcctgctg
     2401 gaacagatcg ccgagaagta agaagtcgtc gcgccgcgcg agccacatgc ccatcggctc
     2461 gaggcgcagc cagtccgcgt tgaccttgac gatgccggcg tagatgtaca tcacctcgaa
     2521 ctgcaggcgg aacagccata ttgcccacgc cggcgtcggc gagcgcaggt gcggcgccgg
     2581 aatcaccatc agtgccaccg agatcaggat caccaggtag aagtggttga ggtagcgcgc
     2641 ctggtcgagc aagaagatgt aggtgaacgc gaggaagaac agcaccgtcg ccgcccggta
     2701 gaacacgccg agcgtgatga ggagcgcgag cacggcgagc gccgcgaagt gccagtacat
     2761 gccgtcgccc ggccaggcct ggacccactc gaagccgtag tacttgaaga agaactcggg
     2821 cgcgatgtag tggcgctcga tccagccctt cgaccagaag cgccagcact ccaccagcat
     2881 gatgacgccg aacatcacgc gcagcgc
//
LOCUS       DATBSZ010000303        20993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_677286, whole genome shotgun sequence.
ACCESSION   DATBSZ010000303 DATBSZ010000000
VERSION     DATBSZ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20993
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677286"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..785
                     /locus_tag="VIG70_19835"
     CDS             108..785
                     /locus_tag="VIG70_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252849.1"
                     /translation="MPSSLFASVVFLKIQDFARRSATEQARLRAQLQAVVAVTAAEIP
                     PAGRVMLDSADGAAIVVLDDPRAALRLAERALTATAAGLPLSAGLNHGALQLAGRKGG
                     EGMTGDGIAVAASVAAFAPAARLLASRAFRNALAEASPGLESLLAPAGTFNDPGLRAH
                     ELFKPDEKAPARRSLRYTAAAMVVALALVGGGIALRNANGNQQPFVDAMMAKYPYWKA
                     LVQRVSY"
     gene            785..1909
                     /locus_tag="VIG70_19840"
     CDS             785..1909
                     /locus_tag="VIG70_19840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8252850.1"
                     /translation="MAKALKKIGRYKILGELGRGAMGVVYKAEDPNLDRTVALKTISL
                     DKDAEGKAEYQKRFFVEAKAAGKLNHPHIVTTFDYGEADGAAYIAMELLEGTDLRKRV
                     QQGPIPAIEAVEIACQVAEGLAYAHHRGIVHRDIKPGNIMLPDRGAAKIMDFGLARMR
                     VADHKTSTGIVLGTPRYMSPEQISGQPVDHRSDVFSLGIVLWEMLTGRRLFSGTEMAQ
                     VSHSITYDEHEPPTRVNPELPAMLDFVVARALKKDPAVRYQDADEMAADLHTCLAEMR
                     AREGEETGDGTKTQKLNVDGEPTIVGPPAAAILTDTRLPVSRHFDSAAALKRLGKSAR
                     FGHAPRPVGLVRRIISDAPARRLFVVTVLAAAAGSFVAFI"
     gene            complement(1938..3326)
                     /locus_tag="VIG70_19845"
     CDS             complement(1938..3326)
                     /locus_tag="VIG70_19845"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252851.1"
                     /translation="MRSLEDVIQAAGNPVDLLRHSPSGPNVYPGVPPEYTNWRDEQQA
                     WQKTCVLFNLSFHMADLYVEGPDATKLLSQLAINTFKNVSPGRAKQFVPCSHDGRVIG
                     DVILFNSAPNKYVLVGRIPALNWIQYHAKTGKYDVKLELDERSAARKDPFNRKSYRFQ
                     IQGPNAMKTMEKVLGAKPPEVKFFHVAPLTIAGKRVTALRHGMAGQPGFELVGPYAEG
                     DAVREAIVKAGEEFGLRLVGGRAYSSNTLESGWIPSPLPAVYSGEKMKPYRQWLTAES
                     YEAKASIGGSFASKKIEDYYLTPWDMGYSSFIDYEHDFIGRDALQEMAKGTHRKKVTL
                     ALEEADVLRVVGSQLEKGERGKFMEFPSAVYSMHPYDKVVAGGKTVGISTWIGYSSNE
                     RRMLTIAVLEAQHAEPGTEVTFVWGEENGGTKKPTVEKHKQMEIRAIVSPVPYAETAR
                     KSYADGGWRKQA"
     gene            complement(3334..4728)
                     /locus_tag="VIG70_19850"
     CDS             complement(3334..4728)
                     /locus_tag="VIG70_19850"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252852.1"
                     /translation="MKSLEEVLRAAGNPVTMLRNSQIGAYVYPVVPGEFSNWRDEQAA
                     WQKTAVLFDQSHHMVEQYVQGPDTLKLFTHLGMNSFANFPVNRAKQLAPTNYDGYVIG
                     DGILFHLEGNHMVFVGRAPAASWIQFHAETGGFNVKTRKDDRSPGNTKGLAINRTNYR
                     FQIQGPNAWKVIEKVNGGPVPELKFFHMATMKIAGRTVRTLRHGMAGAPGLEIWGPYA
                     DREEIRAAIVAAGKEFGLKEVGARAYATNTCESGWIPSPLPAVYTGEKMKKYREWLPA
                     SSYEATGSIGGSFASQNIEDYYVTPYEMGYGPFIKFDHDFIGSEALQKMAGKPHRRKV
                     TLAWNADDVVKVWRSMLEQGDPYKYIDLPLSNFTSATYDRLTHQGKTVGLNMFSGYSY
                     NERSMLSLAIVDPDIQLGNEVRLLWGEEGGGTKKPTVERHKQIEIRAIVSPAPYAKQA
                     RTSYHEGWRSKATA"
     gene            complement(4742..5899)
                     /gene="metK"
                     /locus_tag="VIG70_19855"
     CDS             complement(4742..5899)
                     /gene="metK"
                     /locus_tag="VIG70_19855"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY8252853.1"
                     /translation="MSDFLFTSESVSEGHPDKVADQISDAVLDAILKQDKTSRVAAET
                     LVKDNLVVLAGEITTNARVNYDAIARNTIRRIGYNDPSIGFDAATCTVLIAYGQQSPD
                     IAQGVDEGKGLDLEQGAGDQGLMFGYACDETPSLMPLPIFLSHRLVERQAELRRNGRL
                     PWLRPDAKSQVTIRYVNGKPDSLDTVVLSTQHAKGIEHNKLKESVIEEVIKPVLPKSM
                     LKGKVNYLVNPTGAFEIGGPKGDCGVTGRKIIVDTYGGSAPHGGGAFSGKDPSKVDRS
                     AAYAARYVAKNIVAAGLASKCLVQVSYAIGVARPTSVMVTTQGTGKMSDDALSALVTK
                     HFDLRPKGIIQMLDLQRPIYEKTAAYGHFGREEPEFTWEATDKALALKQAA"
     gene            5948..6835
                     /locus_tag="VIG70_19860"
     CDS             5948..6835
                     /locus_tag="VIG70_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8252854.1"
                     /translation="MPRLPRFLLGLIAGLPLRLLHGLGTVLGWAIYGISPTYRRHLRE
                     NLQHAGYRDAGLRRAAIANAGQMLAETPAMWLRPQQEVAALVSEVHGMDAVDAARAEG
                     KALLFLTPHMGCFEIAAHYAALHVPITVLYRAPKLGWLEPLMREGRGRGGVRLVPADF
                     TGVRELFAALKRREAAGFLPDQVPGAGEGEWAEFFGRLAYTATLAPKLARRDDLACFL
                     AYAKRLPRGAGYSITIRPYPEIRPNEAPARLLNRALEELVRECPEQYLWGYNRYKIPS
                     GAKPKPEAGGEIPPAPPAA"
     gene            6832..7689
                     /locus_tag="VIG70_19865"
     CDS             6832..7689
                     /locus_tag="VIG70_19865"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HEY8252855.1"
                     /translation="MTRLVFVFMWLVRFLPLSILSRIGSAIGAAVFWLIPERRMVTRV
                     NLGKCFPQKSPIERERLARAHFRAFCRAFIEQGILWWSPRSRIRDLVRVEGLEHLKGH
                     PKTIVFAPHFVGFEATLARLGLEFPVAMMYSRQKDPVFEARLLAGRTRFGSLMFPRQA
                     GIKTGIQVIESGTLYYYLPDLDFGPKRSVFVPFFGVPAATVTGLAYIARTTGAAVVPC
                     VTRILPGGGGYLARLYPAWSGFPSGDDHADARRMMAFIEERVLEMPEQYFWLHKRFKT
                     RPEGEPRFY"
     gene            7700..8593
                     /locus_tag="VIG70_19870"
     CDS             7700..8593
                     /locus_tag="VIG70_19870"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8252856.1"
                     /translation="MRLAIIGAGHMGRYHAEKFARLPGVELAAVVDPDPARAQVSDFR
                     KILGKVEAAVVAVPTNLHHEVARACLENGMHVLIEKPIAATLAEADDLVELASKKNAV
                     LQVGHVERYNKAFRALVARMDRPLFIDAERLSGFKQRGAEVDVILDLMIHDLDLALSL
                     ARSEVQAVSACGFRVLTGDTDIASVRIEFANGCVADLTASRVSQAAVRKFRVFQPGVY
                     VSADLQAGKLRYVKQADGVIEETEEAHPGGDALAAQAESFILAITENRPVLVDGAEGR
                     RVLKLALDIGRLVRERLERFA"
     gene            8590..9690
                     /locus_tag="VIG70_19875"
     CDS             8590..9690
                     /locus_tag="VIG70_19875"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY8252857.1"
                     /translation="MSIQIPMVDPAGEYRALKAEIDAAVGRVFESGRFVLGPEGEALE
                     REIAHFIGVEHAVGVNSGTDALHLPLVACGIGPGDEVVIPGFTFFATGEAVSYTGARP
                     VFADVDVGTFNLDPASLKKSITKKTKAVIAVHLFGQCAELDEIARICREKKLVLIEDC
                     AQCIGADYDGRNAGSWGDFGGFSFYPTKNLAAAGDAGLMTARLEEHAKILRMLRHHGS
                     RQTYLHDRVGWNSRLDELQAAVLRVKLKHLAHFNDARRAVARRYREKLAGANVILPAE
                     HGRGAHVYHQFTIRAERRDVIRETLSQEGIASSVFYPMPLHKQPAYESIAKGVSLPVA
                     EEVANTVLSLPIHPLLEEASIDRIAACVRRAA"
     gene            9687..10481
                     /gene="dapF"
                     /locus_tag="VIG70_19880"
     CDS             9687..10481
                     /gene="dapF"
                     /locus_tag="VIG70_19880"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY8252858.1"
                     /translation="MKLAFTKMQGAGNDFVVLDCTRQPLTLNPQQVKKIADRHFGVGC
                     DQILVVERPRSAGADFGYRIFNADGGEVEQCGNGARCFVKFVHARGLTDKREIRVETL
                     GGMIQPRLEADGEVSVDMGKPSTPAVERLNVQGRDVEVAILSMGNPHAVQVVPDVASA
                     PVLAQGPAIETHPRFPHRVNAGYMQVLERHAIALRVWERGAGETLSCGSGACAAVVAG
                     ISRKLLDSPVRVETRGGALTVAWAGGENAVFMKGPAATVFEGSIEL"
     gene            10489..11133
                     /locus_tag="VIG70_19885"
     CDS             10489..11133
                     /locus_tag="VIG70_19885"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEY8252859.1"
                     /translation="MNADDIARYLRTHPQFFDQHPELLEAIAVPHPYGGRAIPLAERQ
                     TVALREKLKLLEGRLAELLQFGEENDAISEKVHRLSVALTGARDFPALATSLYFHLRE
                     DFAVPHVALRVWGKSVPADFDEAQAVDEAQRHHAGTMGAPQCGAAVGNPFVPWFRQAS
                     EHVRSVALVPLGQTTVFGLLALGSEDPKRFYPEMGTLYLRRIGELCAAGVTARL"
     gene            11103..12005
                     /gene="xerC"
                     /locus_tag="VIG70_19890"
     CDS             11103..12005
                     /gene="xerC"
                     /locus_tag="VIG70_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY8252860.1"
                     /translation="MRGRRDSEALTHEYLDALKHQRRLSAATLSNYGRALDVLLELNG
                     KKDLQSLEPAEVRRYVAVLHSKGLSPRSLALALSAWRGCFRWLARHRGFHANPVLGIR
                     APKAARPLPKALSVEGAQRLLEGEREGESSPLGVQDAAMFELLYSSGLRVGELVALDL
                     SDDPAQGEVTVIGKGSKTRTVPVGAKAREALDHWVSVRNSIAEPDEKALFVGARGRRI
                     APSRVWHRLGAWAKRRGIAEHVHPHMLRHSFASHMLQSSQDLRAVQELLGHASISTTQ
                     VYTHLDFQALAKVYDAAHPRAKKK"
     gene            12002..13183
                     /locus_tag="VIG70_19895"
     CDS             12002..13183
                     /locus_tag="VIG70_19895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8252861.1"
                     /translation="MNHLVLKKGRDKSLRRRHPWIFSGAVEKGGGKSGETVEVRDASG
                     KVLAQAAYSPKSQIRARVWTFGAAEAIDADFFRGRIQKALALREALPAAKHANALRLV
                     HGESDGLPGLVVDRYADVLVAQFLAAGVERWRDPILDLLSELSGCEAIFERSDAEVRK
                     LEGLEPRVGFARGNRNASRCPIIEYGLNFRVDVEQGQKTGFFLDQRENRQRVRALAAG
                     REVLDGFCYTGGFSIAALAGGAKRVTAIESSAAALEVAKENLAANPLDASKVEFERAD
                     VFAKLRTLRDQNAKFGLIVLDPPKFAPTAAQVKKAARAYKDINLWALKLLEPGGLLAT
                     FSCSGGVSAELFQSIVAGAALDAEADAKIVERFTAAADHPVALEFPEGEYLKGLLVLK
                     S"
     gene            13184..14170
                     /locus_tag="VIG70_19900"
     CDS             13184..14170
                     /locus_tag="VIG70_19900"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8252862.1"
                     /translation="MRRRTILGLATALLAAPAKLLAQSQRVYRVGAFQFGETPVLRDH
                     MRVFRERLQQLGFAAGRNLQMVEEYSSIDPVARAQSADKLIRAKPDVILSFGSTNTRS
                     IQAASTNKLPVVFTIVGDPVAYGVVKELARPGGNTTGVAFLQREMTVKRLELLREVLP
                     KARRVVVAAYQQDVTYTASEPLVRQIAARLGFELVTAELSGPTPDAAVSKAIEGGADA
                     IFVYQPMSFVVGLEAPEKIVRLATGRRIPVFFAEPDLVVRGGLLSYGPNFLDETRRAA
                     DLVAKVLRGANAGDLPVEQSARFELVVNLKAAKALGIAIPESVRPRIDRVVE"
     gene            14220..15263
                     /locus_tag="VIG70_19905"
     CDS             14220..15263
                     /locus_tag="VIG70_19905"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEY8252863.1"
                     /translation="MIRTLAAALLALLSSAAPAQDKPQPSAAAAAKMRASVERVLAAL
                     PEKSRAQAMRPFEDRDRTDWHYTPRTRNGLSFKEMDKAGRDAVHGLLRTALSAAGYRK
                     VVNIIELEIVLREIETFGWMRDPERYHLTVYGTPDAAGRWGWRFEGHHLSLNFTLAGD
                     KLAVDTPSFFGANPATVQKGAKKGLRALGEEHDAGWALLESLGEAQRREAVFEERTYG
                     DIVTANKDKVEALPAAGIAGAKLDEKQRALLWRLIEIYAGNFEPGLAQARLARAKQGG
                     IEAIRFGWAGSTVRGKPHYYRVQGSLFLIEYDASQNDGNHIHTVWRDFSGDFGRDLLK
                     EHYAAARGTRHRH"
     gene            15263..17065
                     /locus_tag="VIG70_19910"
     CDS             15263..17065
                     /locus_tag="VIG70_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY8252864.1"
                     /translation="MRLAFFFLMFFGLTAWAAPVRTPQAEAELVAERDALVPGEPITV
                     ALRLAAIPGWHTYWRNPGDSGEPTRIEWRLPPGFAAGEIEWPVPVRIPVGPLMNFGYK
                     GEVLLLTRVLPPADLAADRPVTLRAKAFWLVCEVQCLQQDAELSVELPVARKTAEDAR
                     WGKPIAAARERLPVPAPAQWLFAAKAEDEGASLAISPPSGVELKEAFFLPFEEGKVEP
                     AAPQALAREGDGYRLALRAAAQPVGSFDRVSGLLISEKNALAIDVPIVARFADLGLVA
                     ALVLAFAGGLLLNLMPCVLPVLSIKVLGFAQGGDARVSRALLYSAGVLVSFWLLAAAL
                     IALRALGNQLGWGFQLQSPVAVALLAALFLVLALNLSGLFEFRFSFAGGPSGHFLSGV
                     LAVVVASPCTAPFMGAALGYAVFESGWRAFAVFTALGLGMALPYAALAAAPGWRRHLP
                     KPGPWMPRLKQWLAVPLYATVIWLAWVLVQQTTPPSAPHAAWEPYSAEKVAALTAAGK
                     PVFIDFTAAWCVTCQVNERVVLARDDVMQAFRDRGVTLVRADWTRRDPEIARALTALG
                     RSGVPTYAMYKPGRPPELLPELLTPERVLAALDG"
     gene            17089..17679
                     /locus_tag="VIG70_19915"
     CDS             17089..17679
                     /locus_tag="VIG70_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY8252865.1"
                     /translation="MKRLALALVLASASTAWAAKVGQAAPDFSAPDLQGKAVKLSDYR
                     GKYVVLEWTNPECPYVQRHYNSGNMPELQKELGSKDVVWLTVNSTNQSSSEFKTPQQM
                     GKWMGEKGAAPKATLIDKDSQVGKLYDARTTPHMYIVDPQGKLIYAGAIDDKRWASAE
                     ATKQAKNHVRTALGEAMAGKPVSTAATSPYGCTVKY"
     gene            17679..18659
                     /locus_tag="VIG70_19920"
     CDS             17679..18659
                     /locus_tag="VIG70_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8252866.1"
                     /translation="MAEENMVPVTILTGFLGSGKTTLLNRILKEDHGRRIAVIENEFG
                     EVGVDNDIIESGDEQIVEMNNGCICCTVRGDLIRILGTLKEKRDKGDLKFDRVVIETT
                     GMADPGPVAQTFFTDEEIGNYYLLDSIITLVDAKHAPKQLDEFHEAQEQVGFADRILL
                     SKTDLVSEDEAAKLSSRLKKMNPRAPIKKVHFGNAPIDEVLDIRGFNLNAILELDPEF
                     LKSIEHEHHDEVESFVFRSDKPFDGQKLESFLSGMIQVYGPDLLRYKGILWMKGNNRR
                     VVFQGVHMMMGGDMGKPWTKSEKKSSLLVFIGKKLPKDIFIAGLEECLAK"
     gene            18660..19286
                     /locus_tag="VIG70_19925"
     CDS             18660..19286
                     /locus_tag="VIG70_19925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8252867.1"
                     /translation="MIAVRGLAHRYGKHEALRLSEWKVAQGERWLVLGPSGCGKSTLL
                     HVIAGLIQPAEGEVEVSGENLRKLEGARMDRWRGSTVGIVLQALHLVRHLSVRDNLRL
                     AQYLAHVPQDNARIADTLGALGVGEKTARRPSELSQGEQQRVAIARAVVNRPKLLLAD
                     EPTANLDDSAAAKVVELLAEQAARHGATLVVATHDSRVKGKFRERLEL"
     gene            19283..20488
                     /locus_tag="VIG70_19930"
     CDS             19283..20488
                     /locus_tag="VIG70_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8252868.1"
                     /translation="MNLATLSASYLRARPLNTALSLILLALGVATIVLLILVVGQLEE
                     RMQRDARGIDLVVGAKGSPMQLILSGIYHVDAPTGNIPLAAARELGKHRLIRKSIPLA
                     LGDSWRGNRIVGAGREYPEHYGVKMREGRFHEKPMEAVLGAEVAARTGVGVGGSFVGA
                     HGIGGEGEEHKEAPYKVVGVLEKSGSVIDRLVLTSIESVWRVHEEHQGPEDEADRKAL
                     EEAREVTVLLVQYASPLAAAMLPRQINSQSELQAASPAYETARLFSIVGVGVEALRAF
                     ALVLILAAGLSVFIALYTALEERRYDLAVMRTLGASPGKLFGLLLGEGIALSLLGALI
                     GLGLGHAFASALGVWLESQRQSAVTGLEWRPEELWLLGVSLAVGLLAALLPAWRAYRT
                     DVSRTLAQG"
     gene            20498..20965
                     /locus_tag="VIG70_19935"
     CDS             20498..20965
                     /locus_tag="VIG70_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HEY8252869.1"
                     /translation="MKIRILLAALLIVSPAIAQHQTPPGADPNQFKPLPDRNDVVSWK
                     TLSQVELVKQKDRYVPQFAAAVAALDKKEVKVQGFMMPLEMGDKQSHFVLTAMPQSCA
                     FCLPGGPESMVEVKSKAPVKYTFEPVVLSGKLAILKDDPTGVFYRLTDAVPAK"
BASE COUNT         3395 a         6863 c         7153 g         3582 t
ORIGIN      
        1 tatttagctg agcctgaccc cctctgacat tgcaggggac gcaacgctcc tcagcgggcg
       61 aattataccc tgttcaagcg ccgccaatcc ctgcatcctt gcgcgctatg ccgtcgtcgc
      121 tcttcgccag cgtggttttc ctgaagatcc aggatttcgc gcgtcgttcc gccaccgagc
      181 aggcgcgcct gcgcgcgcag ctgcaggcgg tggtggcggt gaccgcggcg gaaattccgc
      241 ccgcgggccg ggtgatgctc gactcggccg acggcgccgc catcgtggtg ctcgacgatc
      301 cgcgcgccgc gctgcgtctc gccgagcgcg cgctgaccgc gactgcggcc gggctgccgc
      361 tttccgccgg cctgaaccac ggcgcactgc aacttgccgg ccgcaagggc ggggaaggca
      421 tgacgggcga cggcatcgcc gtcgccgcgt cggtcgccgc attcgcgccg gcggcgcgcc
      481 tgctggcttc gcgcgcgttc cgcaatgcgc tggccgaggc ttcgccgggg ctggaatcgc
      541 tgctcgcgcc ggcgggtacg ttcaacgacc ccggcctgcg cgcgcacgag ctgttcaagc
      601 ccgacgagaa agcgcccgcg cgccgcagcc tgcgctacac cgccgccgcg atggtcgtcg
      661 cgctcgcgct ggtcggcggc ggcatcgcgc tgcgcaacgc caacggcaat cagcagccgt
      721 tcgtcgacgc gatgatggcg aagtacccgt actggaaagc gctggtgcag cgggtgagct
      781 actagtggcc aaggccctga agaagatcgg ccgctacaag atcctcggcg agctcgggcg
      841 cggcgcgatg ggtgtggtct acaaggccga ggacccgaac ctcgaccgca ccgtcgcgct
      901 caagaccatc agcctcgaca aggacgccga gggaaaggcc gagtaccaga agcgcttctt
      961 cgtcgaggcc aaggcggcgg gcaagctcaa ccacccgcac atcgtcacca ccttcgacta
     1021 cggcgaggcc gacggcgcgg cgtacatcgc gatggagctg ctcgagggca cggatctgcg
     1081 caagcgcgtg cagcaggggc cgatcccggc gatcgaagcg gtcgagatcg cctgccaggt
     1141 ggccgagggg ctggcctatg cgcaccaccg cggcatcgtg catcgcgaca tcaagcccgg
     1201 caacatcatg ctgcccgacc gcggcgcggc gaagatcatg gacttcggcc tcgcacgcat
     1261 gcgcgtcgcc gaccacaaga ctagcaccgg catcgtgctc ggcacgccgc gctacatgtc
     1321 gccggagcag atctcggggc agccggtcga ccaccgctcc gacgtcttct cgctcggcat
     1381 cgtgctctgg gagatgctca ccggccggcg gctcttcagc ggcaccgaga tggcgcaggt
     1441 gagccacagc atcacctacg acgagcacga gccgccgacg cgcgtcaatc cggagctgcc
     1501 ggcgatgctc gatttcgtcg tggcgcgcgc gctgaagaag gacccggcgg tgcgctacca
     1561 ggacgccgac gagatggcag ccgacctgca cacctgcctt gccgagatgc gcgcgcgcga
     1621 aggcgaagag acgggcgacg gcaccaagac gcagaagctc aacgtcgacg gcgagccgac
     1681 gatcgtcggg ccgcccgcgg ccgccatcct caccgatacg cggttgccgg tgtcgcgcca
     1741 cttcgattcc gccgcggcgc tcaagcggct cggaaaatcg gcgcggttcg gccatgcgcc
     1801 gcgaccggtc ggcctcgtcc gcagaatcat cagcgacgcg ccggcgcgcc gccttttcgt
     1861 ggtgacggtc ctggcggccg ccgccggaag cttcgtcgcc tttatctagg ctacggggat
     1921 cgcttagggg atttccctca agcctgcttg cgccagccgc cgtcggcgta ggacttgcgc
     1981 gccgtctcgg cgtagggcac cgggctgacg atggcccgga tctccatctg cttgtgcttc
     2041 tcgaccgtcg gcttcttcgt gccgccgttc tcctcgcccc agacgaaggt gacctcggtg
     2101 ccgggttcgg cgtgctgcgc ctcgagcacg gcgatggtga gcatcctgcg ctcgtttgag
     2161 ctgtagccga tccaggtgga gatgccgacc gtcttgccgc cggcgacgac cttgtcgtac
     2221 ggatgcatcg agtacaccgc cgacgggaac tccatgaact tgccgcgctc gcccttctcg
     2281 agctggctgc cgacgacgcg cagcacgtcg gcttcctcga gcgccagggt cacttttttc
     2341 cggtgcgtcc ccttcgccat ctcttgcaga gcgtcgcggc cgatgaagtc gtgctcgtag
     2401 tcgatgaacg agctgtagcc catgtcccag ggcgtaaggt agtagtcctc gatcttcttc
     2461 gaggcgaagc tgccgccgat cgaagccttg gcctcgtagc tctcggcggt gagccactgg
     2521 cggtagggct tcatcttctc gcccgagtag accgcgggca ggggggaagg gatccagccg
     2581 gattcgagcg tgttggagga gtacgcgcga ccgccgacga ggcgcaggcc gaactcttcg
     2641 ccggctttca cgatggcctc gcgcaccgca tcaccctcgg cgtagggccc caccagctcg
     2701 aagccgggct ggccggccat gccgtggcgc aacgcggtca cccgcttgcc ggcgatggtc
     2761 agcggggcga cgtggaagaa cttcacttcc ggcggcttcg cgccgagcac cttctccatg
     2821 gtcttcatcg cgttcggacc ctggatctgg aagcggtagg acttgcggtt gaacgggtcc
     2881 ttgcgcgccg ccgagcgctc gtcgagctcg agcttcacgt cgtacttccc ggtcttcgcg
     2941 tggtactgga tccagttcag cgccgggatg cggccgacga ggacgtactt gttcggcgcc
     3001 gagttgaaca ggattacgtc gccgatcacg cggccgtcgt ggctgcaggg cacgaactgc
     3061 ttcgcgcgcc ccggcgagac gttcttgaac gtgttgatcg ccagctgcga cagcagcttg
     3121 gtcgcgtccg gtccctcgac gtagaggtcc gccatgtgga acgacaggtt gaagagcacg
     3181 caggttttct gccaagcctg ctgctcgtcg cgccagttgg tgtactccgg cggcacgccg
     3241 ggatagacgt tcgggccgct cggcgaatgg cgcaggagat cgacgggatt tcccgccgcc
     3301 tggatgacgt cctcgaggct cctcatgcgg gcctcaggcc gtcgctttgc tgcgccagcc
     3361 ctcgtggtaa ctggtgcgcg cctgcttggc gtacggcgcc gggctgacga tggcgcgaat
     3421 ctcgatctgc ttgtggcgct cgaccgtggg cttcttcgtg ccgccgcctt cctcgcccca
     3481 cagcagccgc acctcgttgc cgagctggat gtcggggtcg acgatggcga gcgaaagcat
     3541 cgagcgctcg ttgtagctgt agccgctgaa catgttgagg ccgacggtct tgccctggtg
     3601 cgtcaggcgg tcgtacgtgg ccgaggtgaa attcgacagc ggcaggtcga tgtacttgta
     3661 gggatcgccc tgctcgagca tcgagcgcca caccttgacc acgtcgtcgg cgttccaggc
     3721 cagcgtcacc ttgcggcggt gcggcttgcc ggccattttc tgcagcgcct cgcttccgat
     3781 gaagtcgtgg tcgaacttga tgaacgggcc gtagcccatc tcataaggcg tgacgtagta
     3841 gtcctcgatg ttctgcgaag cgaagctgcc gccgatcgag ccggttgctt catagctcga
     3901 cgccggcagc cactcgcggt acttcttcat cttctcgccg gtgtagaccg cgggcagcgg
     3961 tgaagggatc cagccggact cgcacgtgtt ggtcgcatag gcgcgcgcac cgacttcctt
     4021 caggccgaat tccttgccgg cggcgacgat cgcggcgcgg atctcctccc ggtcggcgta
     4081 cggaccccag atctccaggc cgggcgcgcc ggccatgcca tggcgcagcg tgcgtaccgt
     4141 gcggccggcg atcttcatcg tcgccatgtg gaagaacttg agttcgggca ccggcccgcc
     4201 gttcaccttc tcgatcacct tccaggcgtt cggcccctgg atctggaaac ggtagttggt
     4261 gcggttgatc gccaggccct tggtgttgcc tggcgagcgg tcgtccttgc gcgtcttcac
     4321 attgaagccg ccggtctcgg cgtggaactg gatccagctc gccgcgggcg cgcgcccgac
     4381 gaacaccatg tggttccctt cgaggtggaa gaggatgccg tcgccgatga cgtagccgtc
     4441 gtagttggtg ggcgcgagct gcttggcgcg gttgaccggg aaattggcga agctgttcat
     4501 cccgaggtgg gtgaagagct tcagcgtgtc ggggccctgg acgtactgct cgaccatgtg
     4561 gtgcgactgg tcgaagagca ccgcggtctt ctgccaggcg gcctgctcgt cgcgccagtt
     4621 ggagaattcg cccggcacca ccggatagac gtaggcgccg atctgcgagt tgcgcagcat
     4681 cgtgaccgga ttaccggcgg ccctcaggac ctcctcgagg cttttcatga ctcctcctgc
     4741 gctaagcggc ttgcttcagt gcaagggctt tgtcggtcgc ttcccaggtg aattccggct
     4801 cttcgcggcc gaagtgcccg taggcggcgg tcttctcgta gatcggccgc tgcaggtcga
     4861 gcatctggat gatgcccttc ggccgcaggt cgaaatgctt ggtgacgagc gccgagagcg
     4921 cgtcgtcgga catcttgccg gtgccctgcg tggtcaccat gacgctggtc gggcgggcga
     4981 cgccgatggc atacgacacc tgcaccaggc atttcgaggc gaggcccgcg gcgacgatgt
     5041 tcttcgccac gtagcgggcc gcatacgcgg ccgagcggtc gaccttggac gggtccttgc
     5101 ccgagaacgc gccgccgccg tgcggcgccg agccgccgta ggtgtcgacg atgatcttgc
     5161 gcccggtgac gccgcagtcg cctttggggc cgccgatctc gaaggcgccg gtcgggttga
     5221 cgaggtagtt caccttgccc ttgagcatgc tcttcggcag caccggcttg atcacttcct
     5281 cgatcacgct ttccttcaat ttgttgtgtt cgataccctt ggcgtgctgg gtggagagaa
     5341 ccacggtgtc cagggaatcg ggcttgccgt tgacatagcg gatcgtgacc tgggacttcg
     5401 cgtccggccg cagccacggc aagcggccgt tgcgccgcag ctcggcctgg cgctcgacca
     5461 gtcggtgcga caggaagatc ggcagcggca tcaggctcgg cgtctcgtcg caggcgtagc
     5521 cgaacatcag gccctggtcg ccggcgccct gctccagatc gagtcccttg ccttcgtcca
     5581 cgccctgcgc gatgtcgggc gactgctgac cgtaggcgat gagcacggtg caggtcgcgg
     5641 cgtcgaaccc gatcgacggg tcgttgtagc cgatgcgccg gatcgtgttg cgcgcgatcg
     5701 catcgtagtt cacgcgcgca ttcgtcgtga tctcgccggc gagcactacg aggttgtcct
     5761 tgaccagcgt ctcggcggcg acgcgcgaag tcttgtcctg ctttagaatc gcgtcgagca
     5821 cggcgtcgga gatctgatcc gcgaccttgt cgggatggcc ttcggacacc gattcggagg
     5881 taaacagaaa atcgctcatt gcctgcgttt cttctactaa gtgggaaggc cgcaaggata
     5941 ccaaaacttg ccgcgcctcc ccaggtttct cctcggtctc attgcgggtc tgccgctgcg
     6001 gctgctgcac gggttgggaa ccgtcctcgg ctgggcgatc tatgggatat cgcccaccta
     6061 ccgcaggcat ctgcgcgaaa acctgcagca cgccggatat cgcgacgcag gcttgcggcg
     6121 cgcggcgatc gccaacgcag ggcagatgct cgcggagacg ccggccatgt ggctgcggcc
     6181 gcagcaggag gtggccgccc tcgtgagcga agtgcatggc atggatgcgg tggacgccgc
     6241 gcgcgctgaa ggcaaggcgc tgctgttcct tacgccgcac atgggctgct tcgagatcgc
     6301 ggcgcactac gccgcgctcc atgtcccgat caccgtgctc taccgcgcgc ccaagctcgg
     6361 ctggctcgag ccgctgatgc gcgagggccg cggccgggga ggcgtgcgcc tggtgccggc
     6421 cgacttcacc ggcgtgcgcg agctgttcgc cgcgctgaag cgccgcgagg cggcaggctt
     6481 tctgcccgac caggtgccgg gcgcgggcga gggcgaatgg gccgaattct tcggccgcct
     6541 cgcctatacc gcgacgcttg cgccgaagct cgcgcggcgc gacgacctgg cctgctttct
     6601 cgcatacgcg aagcgcctgc cgcgcggcgc cgggtacagc atcacgattc gtccttatcc
     6661 cgagattcgt ccaaatgaag ccccggcacg gctgctcaac cgtgcgctcg aggagctggt
     6721 gcgagaatgc cccgagcagt acctctgggg gtacaaccgc tacaagatcc cgagcggcgc
     6781 aaagcccaaa ccggaagcag ggggagaaat cccccccgca ccccccgccg catgacgcgc
     6841 ctcgtttttg tcttcatgtg gctggtgcgc ttcctgccct tgagcattct ttcgcgcatc
     6901 gggagcgcga tcggcgccgc ggtcttctgg ctgattcccg agcggcgcat ggtcacgcgc
     6961 gtcaacctcg ggaaatgctt tccgcagaag agcccgatcg agcgcgagcg cctcgctcgc
     7021 gcccactttc gcgcgttctg ccgcgcgttc atcgagcagg gcatcctgtg gtggtcgccg
     7081 cgcagccgca tccgggacct ggtgcgcgtc gaaggccttg agcatctgaa agggcacccg
     7141 aaaacgatcg tgttcgcccc gcacttcgtc ggcttcgagg cgacgctggc gcgcctcggc
     7201 ctcgagttcc cggtggcgat gatgtactcg cggcagaaag atccggtctt cgaagcgcgc
     7261 ctgctcgccg ggcgcacgcg cttcggcagc ctgatgttcc cgcgccaggc gggaatcaag
     7321 accggcatcc aggtgatcga gtcggggacg ctctattact atctgcccga cctcgacttc
     7381 ggcccaaagc gctcggtgtt cgtgccgttc ttcggcgtgc ccgccgccac cgtgaccgga
     7441 ctcgcgtata ttgcccgcac gaccggcgcg gcggtggtgc cctgcgtgac gcggatactt
     7501 cccgggggcg gcggctatct cgcccggctg tatcctgcgt ggagcggctt tccgagcggc
     7561 gatgaccacg ccgacgcgcg gcgcatgatg gccttcatcg aggagcgggt gctggagatg
     7621 cccgagcagt acttctggct gcacaagcgg ttcaagacgc gccccgaggg tgagccgagg
     7681 ttctattgag gcgagccgct tgaggttggc catcatcggc gcgggccaca tggggcgcta
     7741 ccacgccgag aagttcgcgc gcctgcccgg ggtcgagctc gccgcggtcg tggaccctga
     7801 tcccgcgcgc gcgcaggttt cggatttcag gaagatattg ggaaaggtcg aggccgcggt
     7861 cgtcgccgtg ccgacgaacc ttcatcacga ggtggcgcgc gcgtgcctcg agaacggcat
     7921 gcatgtcctc atcgagaagc cgattgccgc cacactcgcg gaggccgacg atctggtgga
     7981 gttggcctcg aagaagaacg cggtcctcca ggtcggacac gtcgagcgct acaacaaggc
     8041 gttccgggcg ctcgtggctc gcatggaccg gccacttttc atcgatgccg agcgcctctc
     8101 cgggttcaag cagcgcggcg cggaagtaga cgtgattctc gatctgatga tccacgacct
     8161 cgatctcgcg ctttcgctgg cgcgctccga agtacaggca gtgagcgcat gcggctttcg
     8221 cgtgctcact ggcgacaccg acattgcctc ggtgcgcatc gagttcgcca acggttgcgt
     8281 tgccgacctg acggcgagcc gcgtcagcca ggccgcagtg cgcaaattcc gcgtgttcca
     8341 acccggcgtc tacgtctcgg ccgatctgca ggcgggaaag ctgcgctacg tgaaacaggc
     8401 cgatggcgtg atcgaggaga ccgaggaagc gcaccccggc ggcgacgccc tcgcggccca
     8461 ggctgaaagc ttcattcttg ccattaccga gaatcgtccg gttctggtcg atggcgcgga
     8521 aggccgccgc gtgctcaagc tcgccctcga tatcggccgc ctggtgcgcg agcgcctgga
     8581 gcgattcgca tgagcatcca gattccgatg gtggacccgg cgggcgaata ccgcgcgctg
     8641 aaagccgaga tcgacgccgc ggtcggccgc gtgttcgagt ccgggcgctt cgtgctcggc
     8701 cccgaaggcg aggcgctcga gcgcgagatc gcgcacttca tcggcgtgga gcatgccgtg
     8761 ggggtgaact cgggcaccga cgcgctgcac ctgccgctcg tcgcgtgcgg catcgggccc
     8821 ggcgatgagg tggtgattcc cggattcacc ttcttcgcga ccggcgaggc ggtgtcctat
     8881 accggcgcaa gaccggtgtt cgcggacgtc gatgtgggca ctttcaacct cgatcctgcc
     8941 tcgctgaaaa aaagcatcac caagaagacc aaggcggtca tcgccgtgca ccttttcggc
     9001 cagtgcgccg agctcgacga aatcgcccgc atctgccggg aaaagaagct cgtcctgatc
     9061 gaggattgcg cgcaatgcat cggcgccgac tacgacggca ggaacgccgg cagctggggg
     9121 gatttcggcg gattttcgtt ctatccgacg aagaatctgg ccgctgcggg agacgcgggg
     9181 ctgatgaccg ccaggctcga ggagcacgca aagatcctgc ggatgctgcg tcaccacggc
     9241 agccggcaga cctacctgca cgatcgcgtg ggctggaaca gccggctgga cgagctgcag
     9301 gcggcggtgc tgcgggtgaa gctgaagcac ctggcgcact tcaacgacgc gcgccgcgcg
     9361 gtggcgcgcc gctaccggga gaagctcgcg ggcgcgaacg tgatcctgcc cgccgagcac
     9421 gggcgcggcg cgcacgtcta tcaccagttc accatccgcg ccgagcggcg cgacgtgatc
     9481 cgcgagacgc tgtcccagga aggcatcgcg agctcggtgt tctacccgat gccgctgcac
     9541 aagcagccgg cctacgaaag catcgccaaa ggggtctctc ttccggtcgc ggaagaagtc
     9601 gccaacaccg tgctgtcgct gcccatccac ccgctcctcg aagaggcgtc gatcgaccgc
     9661 atcgcggcct gtgtgcgccg cgcggcatga agctcgcgtt tacaaagatg cagggggcgg
     9721 gcaacgattt cgtcgtcctg gattgcacgc ggcagccgtt gaccctcaac cctcagcagg
     9781 tgaagaaaat cgcagaccgc cacttcggcg tcggctgcga ccagatcctg gtggtggaga
     9841 ggcccaggag cgcgggggcg gacttcggct accgcatctt caacgccgac ggcggcgagg
     9901 tcgagcagtg cggcaacggc gcgcgctgct tcgtcaagtt cgtgcacgcg cgcggactca
     9961 cggacaagcg cgagatccgc gtcgagacgc tcggcggcat gatccagccg cggctcgagg
    10021 ccgacggcga ggtgagcgtc gacatgggca agccgtcgac gccggcggtg gagcgcttga
    10081 acgtgcaggg acgcgacgtc gaagtggcga ttctctcgat gggcaatccg catgcggtgc
    10141 aggtcgttcc ggacgtggcg agcgcgccgg tcctcgcgca gggaccggcg atcgagacgc
    10201 atccgcgctt tccccaccgc gtgaacgccg gctacatgca ggtgctcgag cgccacgcca
    10261 tcgcgctgcg cgtctgggag cgcggcgccg gcgagacgct ttcctgcggc agcggcgcct
    10321 gcgcggcggt ggtcgcgggg atctcgcgca agctgctcga ttcgccggtg agggtcgaaa
    10381 cacgcggcgg agcgttgacc gtcgcttggg ccggcggcga gaatgccgtg ttcatgaagg
    10441 gacccgcggc gacggtgttc gaaggatcga tagaactata agaggccaat gaacgcagac
    10501 gatatcgccc gctacctccg cacccatccg cagttcttcg accagcatcc cgagctcctc
    10561 gaggcgatcg ccgtgccgca cccgtacggc gggcgcgcga tcccgctcgc cgagcgccag
    10621 acggtggcgc tgcgcgagaa gctgaagctg ctcgaggggc gcctcgccga gctgctccag
    10681 ttcggcgagg agaacgacgc gatcagcgag aaggtgcacc gcctgtcggt cgcgctcacg
    10741 ggcgccaggg actttccggc gctcgcaacc tcgctctact tccacctgcg cgaggacttc
    10801 gcggtgccgc acgtggcgct gcgggtctgg ggcaagagcg taccggcgga tttcgacgaa
    10861 gctcaagccg tggacgaggc gcagcgccac cacgccggca ccatgggcgc gccgcagtgc
    10921 ggcgccgccg tcggcaaccc gttcgtgccc tggttccgcc aagcttccga gcacgtgcgc
    10981 tcggtggcgc tggtgccgct cggccagacc acggtgttcg gcctgctcgc gctgggcagc
    11041 gaggacccaa agcgctttta cccggagatg ggaacgctct atctccggcg catcggagag
    11101 ctatgcgcgg ccggcgtgac agcgaggctt tgacgcacga gtatctggac gcgctgaaac
    11161 accagcgacg gctgtccgcc gcgacgctct cgaactacgg ccgcgcgctc gatgttcttc
    11221 tcgaattgaa tggcaagaag gatctgcagt cgctggagcc ggccgaggta cggcgctacg
    11281 tcgcggtact gcactcgaag ggcctctcgc cgcgctcgct cgcgcttgcg ctttccgcct
    11341 ggcgcggctg cttccgctgg ctcgcgcgcc accgcggctt tcacgccaac ccggtgctcg
    11401 gcattcgcgc gccgaaagcc gcgcggccgt tgccgaaagc gctctcggtc gaaggagcgc
    11461 aacgccttct cgagggcgaa cgcgaaggcg aaagctctcc cctcggcgtt caggacgcgg
    11521 cgatgttcga gctcctgtat tcgtccgggt tgcgggtcgg cgagctcgtg gcgctcgacc
    11581 tgagcgacga tcccgcgcag ggcgaagtca cggtgatcgg caaaggctcg aagacgcgca
    11641 ccgtgccggt cggggccaag gcacgcgagg cgctcgatca ctgggtttca gtgaggaact
    11701 ctattgcaga gcctgacgaa aaagcgctct tcgtcggcgc ccgcggccgg cgaatcgcgc
    11761 cgagccgcgt ctggcatcgc ctgggcgcgt gggcgaagcg ccgcggcatc gccgagcacg
    11821 tgcacccgca catgctgcgc cactcgttcg ccagccacat gctgcagtct tcgcaggacc
    11881 tgcgcgcagt gcaggagctg ctcggacatg cgagcatctc cactacgcag gtctacaccc
    11941 acctcgattt ccaggcgctc gccaaggtct acgacgcggc gcacccgcgc gcaaagaaaa
    12001 agtgaatcat ctggttctca agaaagggcg cgacaaatcg ctcaggcggc gccacccgtg
    12061 gatcttctcc ggggcggttg agaaaggcgg cggaaaatcc ggcgagaccg tggaagtgcg
    12121 cgacgcatcc ggcaaagtgc tcgcccaagc cgcctacagc ccgaaatcgc agatccgcgc
    12181 gcgcgtctgg acgttcggcg cagccgaagc gatcgacgcg gatttcttcc gcggccgcat
    12241 ccaaaaggcg ctcgcgctgc gcgaggcgct gcctgccgcg aagcacgcaa acgccctgcg
    12301 cctcgtgcac ggcgagtccg acgggttgcc cggcctcgtg gtcgaccgct acgccgacgt
    12361 gctggtcgcg cagttcctcg ccgccggggt cgagcgctgg cgcgacccga tcctggatct
    12421 cctgagcgag ctttcgggct gcgaggcgat cttcgagcgc tccgacgccg aggtgaggaa
    12481 gctcgaaggg ctcgagccgc gcgtcggctt cgcgcgcggc aaccgcaacg cctcgcgctg
    12541 cccgatcatc gagtacggcc tcaacttccg cgtcgacgtc gagcaagggc agaagaccgg
    12601 gttcttcctc gaccagcgcg aaaatcggca gcgcgtgcga gcgctcgccg cgggccgcga
    12661 agtgctcgac ggtttctgct acaccggcgg cttctcgatc gccgcgctcg ccggcggcgc
    12721 gaagcgcgtg accgcgatcg agagctccgc cgcggcgctc gaggtcgcca aggaaaatct
    12781 tgcggcgaat cctctcgacg cctccaaggt ggagttcgaa cgggccgacg tgttcgccaa
    12841 gctccggacc ctaagagatc aaaacgccaa attcggcctg atcgtgctcg atccgcccaa
    12901 gttcgcgccg acggcggcgc aggtgaaaaa agcagcgcgg gcctacaagg acatcaacct
    12961 gtgggccttg aagctgctcg agcccggcgg gctgctggcc accttctcgt gctcgggcgg
    13021 ggtctccgcg gagctctttc aatcgatcgt cgccggcgcg gcgctcgacg ccgaggccga
    13081 cgcgaagatc gtcgagcgct ttaccgccgc ggccgatcac ccggtggcgc tcgagtttcc
    13141 ggaaggggaa tacctgaagg gcttgctggt gctgaagtcc tgaatgcgac gccggaccat
    13201 actcggactc gcgactgcgc tgctcgcggc gccggcaaag ttgcttgcgc agtcgcagcg
    13261 ggtctaccgc gtcggcgcgt tccagttcgg cgagacgccg gtgctgcgcg atcacatgcg
    13321 agtgttccgc gagcgcctcc agcaattggg attcgccgca ggacgcaacc tgcagatggt
    13381 ggaagaatat tcatcgatcg atccggttgc tcgcgcacag agcgcggaca agctgattcg
    13441 cgccaagcca gacgtgattc tttcgtttgg ctcgaccaac acacgatcga ttcaagccgc
    13501 atccacaaac aagcttcccg tcgtattcac gatcgtcggc gatccggtcg cgtacggagt
    13561 cgtcaaggag ctcgcgcgtc ctggcggcaa tacgaccgga gtggccttcc ttcagcgcga
    13621 aatgacggta aagcgtctgg aactgcttcg tgaggttctg ccgaaggcca gacgagttgt
    13681 cgttgccgct taccagcagg atgttaccta caccgctagt gaaccccttg ttcggcagat
    13741 cgctgcccga ctcggattcg aactggttac ggcggagctt tccggaccga cgcccgatgc
    13801 ggccgtcagc aaagcgattg agggcggtgc ggatgcgata tttgtctacc aaccgatgag
    13861 cttcgtcgtg ggcctggagg cgcctgaaaa aattgtgcgg cttgccacgg ggcgacggat
    13921 tcccgtgttc tttgcggaac cggatttggt cgtgcgggga ggcttacttt catacggtcc
    13981 gaatttcctt gatgaaacgc ggcgagcggc cgatctggtt gcgaaggtac tgaggggcgc
    14041 aaacgccgga gatctcccgg tggagcaaag cgcacgattc gagctggtgg tgaatctgaa
    14101 agcggcgaaa gcgctcggca ttgccatccc ggagtcggtg cggcctcgta tcgatcgcgt
    14161 agttgaatga gtgtctgctc ggcggaatat cgcaaggggt tgctgctgtt aaggacagca
    14221 tgatccggac tctggctgca gcccttctcg ccctcctcag cagcgcggcg ccggcgcaag
    14281 acaagccgca acccagtgcc gcggcggctg cgaagatgcg cgcgtcggtc gagcgcgtgc
    14341 tggccgcact gccggagaag agccgcgcgc aggcgatgcg gcccttcgag gaccgcgacc
    14401 gcaccgactg gcactacacg ccgcgcaccc gcaacggact ttccttcaaa gagatggaca
    14461 aggccgggcg cgatgcggtg cacggcctgc tgcgcacggc actgtcggcc gcgggctacc
    14521 gcaaggtcgt gaacatcatc gagctcgaga tcgtgctgcg cgagatcgag accttcggct
    14581 ggatgcgcga tccggagcgc taccacctca cggtgtacgg cacgccggat gccgccgggc
    14641 gctggggctg gcgcttcgaa ggccaccacc tgtctctcaa cttcacgctc gcgggcgaca
    14701 agctggccgt cgatacgccg agcttcttcg gcgccaaccc ggccacggtc cagaagggcg
    14761 cgaagaaagg actgcgcgct ctcggcgagg agcacgacgc gggttgggcg ctcctcgaat
    14821 cgctcggcga ggcgcagcgc cgcgaagcgg tgttcgagga gcgcacctac ggcgacatcg
    14881 tcacggccaa caaggacaaa gtggaggcgc ttcccgccgc gggtatcgcg ggtgcgaagc
    14941 tggacgagaa gcagcgcgcg ctgctgtgga ggctgatcga gatctacgcc ggcaacttcg
    15001 agcccggcct cgcgcaggcg cgccttgcgc gcgcgaagca gggcggcatc gaggcgatcc
    15061 gcttcggctg ggccggctcg accgttcgcg gcaagccgca ctactaccgc gtgcagggat
    15121 cgctgttcct gatcgaatac gacgcgtccc agaacgacgg caaccatatc catacggtat
    15181 ggcgtgactt cagcggcgac ttcggccgcg acctgctcaa ggagcactac gccgcggcgc
    15241 gcggcacccg ccaccggcac tgatgcggtt agccttcttc tttctcatgt ttttcggttt
    15301 aactgcgtgg gcggcgccgg tgcgcacgcc tcaagcggag gccgaactcg tcgccgagcg
    15361 cgacgcgctg gtgccgggcg agccgatcac ggtcgcgctg cgcctcgcgg cgattcccgg
    15421 atggcacacc tattggcgca atcccggcga ttccggcgag ccgacgcgca tcgagtggcg
    15481 gctgccgccg ggttttgccg cgggcgagat cgaatggccg gtgccggtcc gcatcccggt
    15541 cgggccgctg atgaacttcg gctacaaggg cgaggtgctg ctcttgaccc gcgtgttgcc
    15601 gccggcggat ctcgccgcgg atcggccggt gacgctgcgt gcgaaggcat tctggctggt
    15661 gtgcgaggtg cagtgcctgc agcaggatgc cgagctctcg gtcgagctcc ccgtagcgag
    15721 gaaaaccgca gaggacgcgc gctgggggaa gccgatcgcg gcggcgcgtg agcggcttcc
    15781 ggtccccgcg ccggcgcaat ggctgtttgc cgcgaaagcc gaggatgaag gcgcgtcgct
    15841 cgcgatctcg ccgccgtcag gcgtcgagct gaaggaagcg ttcttcctgc cgttcgagga
    15901 aggcaaagtc gagccggcgg cgccgcaggc gcttgcgcgc gaaggcgacg gctatcgcct
    15961 ggcgctgcgt gcggcggcgc agccggtggg atctttcgac cgggtctccg ggcttcttat
    16021 ctcggaaaag aatgctctgg cgatcgacgt gccgatcgtc gcgcgctttg cggacctggg
    16081 tctcgtggct gcgctcgtgc tcgccttcgc gggcgggctg ctcctcaact tgatgccttg
    16141 cgtgctgcca gtgttgtcga tcaaggtgct cggttttgcc caaggcgggg acgcgcgggt
    16201 ttcccgtgcg ttgctttaca gcgcgggtgt gctcgtctcg ttctggctac tcgccgcggc
    16261 gttgattgca ttgcgcgcgt tgggtaatca gcttggctgg ggattccagc ttcagtcgcc
    16321 ggtggcggtg gcgctgctcg cggcgttgtt cctggtgctc gcgctgaacc tgtcgggtct
    16381 cttcgagttc cgcttttcct tcgccggcgg cccgagcggc cactttctct cgggcgttct
    16441 cgcggtggtg gtcgcctcgc cctgcactgc gccgttcatg ggcgcggccc tcggctacgc
    16501 agtgttcgag tcgggctggc gcgcgttcgc ggtgttcacc gcgctcgggc tgggaatggc
    16561 gctgccttac gcggcgctcg ccgcggcgcc gggatggcgc cgccatctgc cgaaaccggg
    16621 gccgtggatg ccgcgtctca agcaatggct ggcagtgccg ctctacgcta cggtgatctg
    16681 gctcgcatgg gtcctggttc agcagaccac gccgccgtct gcgccgcacg cggcatggga
    16741 accctactct gcggagaagg tcgccgcgct caccgccgcc ggcaagccgg tgttcatcga
    16801 tttcaccgct gcctggtgcg ttacttgcca ggtgaacgag cgcgtggtcc tcgcgcgcga
    16861 cgacgtgatg caggccttcc gcgaccgcgg cgtaacgctg gtgcgcgccg actggacgcg
    16921 gcgcgatccc gagattgcgc gcgcgctcac cgcgctcggc cgcagcggcg tgccgaccta
    16981 tgcaatgtac aagccggggc gcccgcccga gctgctgccc gagctcctga cgccggagcg
    17041 cgtgctggcg gcgctggacg gatagaatcc cattctctgg aggtcaacgt gaaacgactc
    17101 gctcttgcat tggttcttgc ttcagcttca accgcttggg ctgcgaaggt cggccaggcc
    17161 gcgcccgatt tttccgcccc ggacctgcag ggcaaggcgg tcaagctctc cgactaccgc
    17221 ggcaagtacg tggtcctcga gtggaccaac cccgaatgtc cgtacgtgca gcgtcattac
    17281 aacagcggca acatgccaga gctgcagaag gagctcggct cgaaggacgt ggtctggctg
    17341 accgtcaact cgaccaacca gtcctcgagc gagttcaaga cgccgcagca aatgggcaag
    17401 tggatgggcg agaaaggcgc ggcgcccaag gcgacgctga tcgacaagga cagccaggtc
    17461 ggcaagctgt atgacgcgcg caccacgcct cacatgtaca tcgtcgaccc gcaaggcaag
    17521 ctgatctatg cgggcgcgat cgacgacaag cgctgggcga gcgccgaggc gacgaagcag
    17581 gcgaagaacc acgtgcgcac cgcgctcggc gaggcgatgg ccggaaagcc ggtgagcacg
    17641 gcggcgacct cgccgtacgg ctgcacggtg aagtactgat ggccgaagag aacatggtgc
    17701 cggtgacgat cctcaccggc ttcctcggca gcggcaagac cacgctgctc aaccgcatcc
    17761 tcaaggagga ccacggccgg cgcatcgcgg tgatcgagaa cgagttcggc gaggtcggcg
    17821 tcgacaacga catcatcgag tcgggcgacg agcagatcgt cgagatgaac aacggctgca
    17881 tctgctgcac ggtgcgcggc gacctgatcc gcatcctcgg cacgctcaag gagaagcgcg
    17941 acaagggcga cctcaaattc gaccgcgtgg tgatcgaaac caccggcatg gccgatccgg
    18001 gcccggtggc gcagaccttc ttcaccgacg aggagatcgg caactactac ctgctcgact
    18061 cgatcatcac gctggtcgac gccaagcacg cgcccaagca gctcgacgaa ttccacgaag
    18121 cgcaggagca ggtggggttt gcggaccgga ttctgctttc caagaccgat ctggtttcgg
    18181 aagacgaagc ggcgaaactc tcttcccgtt tgaagaaaat gaacccgagg gcaccgatca
    18241 agaaggtgca cttcggcaac gcgccgatcg acgaggtgct cgacatccgc ggcttcaacc
    18301 tgaacgcgat cctcgagctc gacccggaat tcctcaagag catcgagcac gagcaccacg
    18361 acgaggtgga gtcgttcgtg ttccgctcgg acaagccgtt cgacggccag aagctcgaga
    18421 gcttcctctc cgggatgatc caggtgtacg gcccggacct gctgcgctac aagggcatcc
    18481 tgtggatgaa gggcaacaac cggcgcgtcg tgttccaggg cgtgcacatg atgatgggcg
    18541 gcgacatggg caagccgtgg acgaaaagcg agaagaagag ctcgcttctc gtgttcatcg
    18601 gcaagaagct gccgaaggac atcttcatcg ccggcctcga ggagtgtctc gctaagtaaa
    18661 tgattgcggt tcgcggtctt gcgcatcgct acggcaagca cgaagcgctg cgcctttccg
    18721 aatggaaggt cgcgcagggc gagcgctggc tggtgctcgg gccgtcgggc tgcggcaaga
    18781 gcacgctgct gcacgtgatc gccgggctga tccaacccgc ggagggcgaa gtcgaggtct
    18841 ccggcgagaa cctgcgcaag ctggagggcg cgcgcatgga ccgctggcgc ggctcgactg
    18901 tcggcatcgt gctgcaggcg ctccatctcg tccgccatct cagcgtgcgc gacaacctgc
    18961 gcctcgcgca gtacctcgcg catgtgccgc aggacaacgc gcgcatcgcg gatacgctgg
    19021 gcgcgctggg ggtgggtgaa aagacggcgc ggcggccgag cgagctgtcg cagggcgaac
    19081 agcaacgcgt cgcgattgcg cgcgccgtgg tcaaccggcc caagctgctg ctcgccgacg
    19141 aacccaccgc caacctcgac gattccgcgg cggcgaaggt cgtggagctg cttgccgagc
    19201 aggcggcgcg ccacggcgcg acgctcgtgg tggccacgca cgactcgcgc gtgaagggca
    19261 agtttcgcga aaggctggag ctttgaatct cgccacgctt tcggcgagct acctgcgcgc
    19321 gcggccgctg aacaccgcgc tcagcctgat cctgctcgcg ctcggcgttg cgaccatcgt
    19381 gctgctgatc ctggtcgtcg gccagctcga ggagcgcatg cagcgcgacg cgcgcggcat
    19441 cgacctcgtg gtcggcgcga agggcagccc gatgcagctc atcctgtcgg gcatctatca
    19501 cgtcgacgcg ccgaccggga acatccccct cgccgccgcg cgcgagctcg gcaagcatcg
    19561 cctgatccgg aaatcaatcc cgctcgcgct cggcgatagt tggagaggaa atcgcatcgt
    19621 cggcgccggc agggagtatc cggagcacta cggcgtgaaa atgcgggaag gacgctttca
    19681 cgagaagccg atggaggcgg tgctcggcgc cgaggtggcg gcgcgcaccg gcgtgggcgt
    19741 gggtggcagc ttcgtcggtg cgcacggtat cggcggagaa ggcgaggagc acaaggaagc
    19801 gccttacaag gtcgtcggcg tcctggaaaa gagcggctcg gtgatcgatc gcctggtgct
    19861 caccagcatc gagagcgtgt ggcgggtgca cgaggagcac cagggaccgg aggacgaggc
    19921 cgatcgcaag gcgctcgaag aggcgcgcga agtgacggtg ctcctcgtcc agtatgcatc
    19981 gccgctcgcc gcggcgatgc ttccgcggca gatcaattcc cagagcgagc tgcaggcggc
    20041 gtcgccggcg tacgagaccg cgcgcctttt cagcatcgtc ggcgtcggcg tcgaagcgct
    20101 gcgcgccttc gccctcgtgc tgatccttgc cgccggcctg tcggtgttca tcgcgcttta
    20161 caccgcgctc gaggagcgcc gctacgacct tgccgtgatg cgcacgctcg gcgcatcgcc
    20221 gggcaagctt ttcggcctgc tgctgggcga ggggatcgcg ctttccctcc tcggggccct
    20281 gatcggcctc ggcctcggcc acgctttcgc ttcggccctg ggcgtttggc tcgagtccca
    20341 gcggcagtct gcggtgacag gattggaatg gcggcccgaa gagctgtggc tgcttggggt
    20401 gtccctcgcg gtgggcctgc tggcggcact gttaccggcc tggcgagctt atcgcaccga
    20461 tgtatcgcgg accttggcgc aggggtagga ttacaggatg aagatccgaa tcctgctcgc
    20521 cgcgttgctg atcgtctccc ccgccatcgc gcagcaccag acgccgcccg gcgcggaccc
    20581 gaaccagttc aagccgcttc cggatcgcaa cgacgtggtg tcgtggaaga cgctgtcgca
    20641 ggtcgagctg gtgaagcaga aggaccgcta cgtgccgcag ttcgccgcgg cggtcgccgc
    20701 gctggacaag aaggaagtga aggtgcaggg gttcatgatg ccgctggaga tgggcgacaa
    20761 gcagagccac ttcgtgctga cggcgatgcc gcaaagctgt gccttctgcc tgcccggcgg
    20821 tcccgagagc atggtcgagg tgaagagcaa ggcgccggtg aagtacacct tcgagccggt
    20881 ggtgctctcg ggcaagctcg cgatcctcaa ggacgatccg acgggcgtgt tctaccgcct
    20941 gaccgacgcg gttcccgcca agtaaagtgc ctgcccgtct cgcgcgggtc ctt
//
LOCUS       DATBSZ010000304         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1975482, whole genome shotgun sequence.
ACCESSION   DATBSZ010000304 DATBSZ010000000
VERSION     DATBSZ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1975482"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VIG70_19940"
     CDS             complement(<1..480)
                     /locus_tag="VIG70_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8252870.1"
                     /translation="MLRLLLLFASRRGRGRMVLSVAGIALGVALGYGVHLVNRAAVSD
                     VAAAVRSLAGEADLEVRGGRAGFPEALYPQIAQLPGVAVASPALELDVGVAGSERTLR
                     VNGIDIMRAALVQPNLILDDRYELLAPDRVFLSVAAAQSLGLGKGDTLPLVVGQTITE
                     "
     gene            501..1466
                     /locus_tag="VIG70_19945"
     CDS             501..1466
                     /locus_tag="VIG70_19945"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HEY8252871.1"
                     /translation="MRVFVFLLVLSLNPSFAQEPPAWFSESLLDLREDVAEAAAQGKR
                     VMLYFGQDGCPYCKRLMEVNFRQAAIAAKAQRQLVALAINIWGDREVTWTDGAVTTEK
                     RLAAQLKVQFTPTLVFLDERGAIALRLNGYHPPHQFDAALDHVAGKISFTEYLERQGR
                     EPASASLHPQPFFLPPPYDLRRGPGAKPLAVLFETPFCAQCDEMHAVAFKRPEVREQL
                     AKFDVVRLAFSSGEPVVAPDGKKISPADWASALRVGYVPTLLLFDARGREVFRTEAYL
                     RPFHIAGALDYVSSGSYVREPSFQRFLQEKAERMRGRGEPVDLWN"
     gene            1512..1937
                     /locus_tag="VIG70_19950"
     CDS             1512..1937
                     /locus_tag="VIG70_19950"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HEY8252872.1"
                     /translation="MARVFLSVLLFALGGCEPLAIALVGAGAGTALRYNIDGVTSRTF
                     TAPAAEVKNASLAAIERMGLAFESLEMFEHGELVYARTENRTIEIEIEPISPRATRMR
                     ITAKNGSYFFYDSATASEIVAQTERSLSHAAGGGTAPNN"
     gene            complement(1950..2459)
                     /locus_tag="VIG70_19955"
     CDS             complement(1950..2459)
                     /locus_tag="VIG70_19955"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEY8252873.1"
                     /translation="MGSRLLLRDLTELYAHALVVEREAAKRFVELERYMRDAGMDHIA
                     DEFESIGKEEREQYELLAIGTSGREMPQLRSWEYAPHFLGPQADAVRAPRSVREALTL
                     AIAIERRTQNFYVDVAEHTTDDTMCAFAAEMAADESRHIARLERLLAREPEAQGTVAG
                     DGDVLPLKT"
BASE COUNT          402 a          875 c          892 g          425 t
ORIGIN      
        1 ttctgtgatg gtttgaccca caacgagggg gagcgtgtcg cccttgccaa ggccaagact
       61 ctgggctgcc gcgaccgaga gaaaaacgcg atccggcgcg agcagctcgt agcgatcgtc
      121 gaggatcagg ttcggctgca cgagcgcggc gcgcattatg tcgatgccgt tgacgcgcag
      181 cgtgcgctcg ctgcccgcga ccccgacgtc gagctcgagc gccgggctcg ccaccgccac
      241 gcccggaagc tgcgcgattt gcggatagag cgcttcgggg aagccggcgc ggccgccgcg
      301 cacctcgagg tcggcctcgc cggcgagcga gcgcaccgcc gccgcgacgt cgctcaccgc
      361 ggcccggttg accaggtgca cgccgtaccc gagcgcgacg ccgagggcga tgcccgcgac
      421 cgagagcacc atgcgcccgc ggccgcggcg cgaggcgaag agcaggagca gccgcagcat
      481 cggcttacta tactgtgagc atgcgggtgt tcgttttctt gcttgttctg agcctcaacc
      541 cctcgtttgc gcaagagccc cctgcgtggt tcagcgagtc gctgctcgac ctgcgcgagg
      601 acgtggccga ggccgcggcg cagggcaagc gcgtgatgct ctatttcggc caggacggct
      661 gcccgtactg caagcgcctc atggaggtca atttccgcca ggctgcgatc gccgcgaagg
      721 cgcaacgcca gctcgtcgcg ctcgcgatca acatctgggg cgaccgcgag gtgacctgga
      781 ccgacggcgc agtgacgacc gagaagcggc tggccgccca gctcaaggtg cagttcacgc
      841 cgacgctggt gttcctcgac gagcgcggcg cgatcgcgct gcgcctgaac ggctaccacc
      901 cgccgcatca gttcgatgct gcgcttgatc acgtggcggg caagatttcc ttcacggaat
      961 atctggagcg ccaaggtcgc gagcccgcga gcgccagcct ccatccccag cccttcttcc
     1021 tgcccccgcc ctacgacctg cgccgcgggc cgggggcgaa gccgctggcg gtgctgttcg
     1081 agacgccctt ctgcgcccag tgcgacgaga tgcacgccgt ggccttcaag aggccggagg
     1141 ttcgcgagca gctggcgaag ttcgacgtcg tgcgcctggc gttttcgagc ggggaaccgg
     1201 ttgtggcccc tgacggcaag aaaatctccc ccgccgactg ggcgagcgcg ctgcgcgtcg
     1261 ggtatgtccc gacactgctg ctgttcgacg cgcgcgggcg cgaagttttt cgcaccgagg
     1321 cctatcttcg gccgttccat atcgcgggcg cgctcgacta cgtgtcgagc ggcagctacg
     1381 tgcgcgagcc ttcgttccag cgcttcctgc aggagaaggc cgagcgcatg cgcggccgcg
     1441 gcgagccggt ggacctctgg aactagtttc gccggatagc ggcttcttct acagaccgtc
     1501 actgtgccgg cttggcacga gtattcctat ccgtactgct gttcgctctc ggcggctgcg
     1561 agccgctcgc gatagcgctg gtcggcgcgg gcgccggcac cgccctgcgc tacaacatcg
     1621 acggggtcac ctcgcgcacg ttcaccgcgc ccgcggccga ggtaaagaac gcttcgctgg
     1681 cggcgatcga gcgcatggga ctcgcgttcg aatcgttgga gatgttcgag cacggcgagc
     1741 tggtctacgc gcgtaccgag aaccgcacca tcgagatcga gatcgagccg atcagcccgc
     1801 gcgcgacgcg catgcgcatc acggcgaaga acggcagcta cttcttctac gacagcgcca
     1861 ccgcgagcga gatcgtggcg cagactgagc gctcgctctc ccacgcggcg ggcggcggga
     1921 cggcgcccaa caactgaccg caggagtggc tacgtcttga gcggcaggac gtcgccgtcg
     1981 ccggcgaccg taccctgcgc ctcgggttcg cgcgcgagca ggcgctcgag gcgcgcgatg
     2041 tggcggctct cgtcggcggc catctcggcg gcgaaggcgc acatggtgtc gtcggtggtg
     2101 tgctcggcca cgtcgacgta gaagttctgc gtgcgccgct cgatggcgat ggcgagtgtg
     2161 agcgcctcgc gcacgctgcg cggcgcgcgt accgcgtcgg cttgcgggcc gaggaagtgc
     2221 ggcgcgtact cccaggagcg aagctgcggc atctcgcggc cggaggtgcc gatggcgagc
     2281 agctcgtact gctcgcgctc ctctttaccg atgctctcga actcgtcggc gatgtggtcc
     2341 atgccggcat cgcgcatgta gcgctcgagc tcgacgaagc gcttggcggc ctcccgctcc
     2401 actaccaggg cgtgcgcgta gagctcggtc aggtcacgaa gaaggagtcg ggaacccatg
     2461 gcgtaaccct attcggtgca ggttacgcac gaccatggtg cggcgcgcta gatcatgggt
     2521 cgaagttcct aatcacctcg cccgaaccgc actgtcgccg aatatatttc gcgccgtcga
     2581 aggcctgtag tccg
//
LOCUS       DATBSZ010000305         9036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1908444, whole genome shotgun sequence.
ACCESSION   DATBSZ010000305 DATBSZ010000000
VERSION     DATBSZ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9036
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908444"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VIG70_19960"
     CDS             complement(<1..215)
                     /locus_tag="VIG70_19960"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY8252874.1"
                     /translation="MKKIAVLTSGGDAPGMNAAVRAVTRGALARGWEVFGVRNGYAGL
                     LGDLIEPLAARDVGGIVQSGGTVLGSAR"
     gene            complement(241..1080)
                     /locus_tag="VIG70_19965"
     CDS             complement(241..1080)
                     /locus_tag="VIG70_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8252875.1"
                     /translation="MRLGVDVGGTKIAIAVLDAGGAERLRRRVEYPSREYGTALELLA
                     KEVLQAEKDAGARCSVGVGMPGLIDPDTGRVENAYNTPYNKKPLKKQLEERLGREVRF
                     GNDANCFALSEAVDGEARGARVVFGAILGTGVGGGIVVGGRMLEGRHGIAGEWGHTPL
                     PWMSDEEHPGPRCTCGRLGCIEQFLSGPAKNRERDEIGEERAMARYEDRLARAFSMII
                     NVLDPDVIVVGGGLSKHERLYANVPPLLKKYVYCEDPRTPIVRARHGEASGVRGAAML
                     WPL"
     gene            complement(1059..1799)
                     /gene="otsB"
                     /locus_tag="VIG70_19970"
     CDS             complement(1059..1799)
                     /gene="otsB"
                     /locus_tag="VIG70_19970"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY8252876.1"
                     /translation="MPPFDRNWAFFLDLDGTLLEIADTPEAVDPGEPEIILLGRLKNA
                     CGGAVALISGRSLSRIDQLFSPLILAAAGQHGAERRDAMGTRHRHRFPVTALRPAAGG
                     IRSFAARHQGLVFEDKGASVALHYRLAPQLAAAAQKAVREAAEPLGDQVEVQGGKMVV
                     ELKPAGCDKGKAIESFMQEPPFAGRVPVFLGDDVTDEYGFRVVNRLGGHSLKVGEGPS
                     AARWRLENPAAARAWLQAWLEKCGSAST"
     gene            complement(1806..4064)
                     /locus_tag="VIG70_19975"
     CDS             complement(1806..4064)
                     /locus_tag="VIG70_19975"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY8252877.1"
                     /translation="MPLSLRFVVPLALALAAIAYAVVPLVDRLTFQWFVRDLDMRGAL
                     IVQTAQDPLVELLRSGRGARERVLRYFDRIAEGQRVFALGFCDPAGKLLYATRVFPEA
                     VRCAKAERREPRNFVLELTDGALHVSAQPVAADGQVLGELMIVHDMSFAQRRSEDTRK
                     YVLWLFAGIAAVVALITVVIAEISWRGWVNGIKAVLSGDYLLRSPAAKAAAAGLRPEL
                     RPIARDLQALVSDLESERRSRDESQTTWGPDALRRILRQDLKGDEVLIVSNREPYLHV
                     RRKDNVIEIQRPASGLVTALEPVMRACSGTWIAHGAGSADRDTVDKHDHVMVPPEKPS
                     YRIRRVWLSQEEEQGYYFGFANEGLWPLCHIAHTRPIFRAPDWEHYQTVNRRFADVVA
                     DEARTDDPIVLVQDYHFALLPKMVRERLPRATIITFWHIPWPNPEAFGICPWREEVLE
                     GMLGSSILGFHTQFHCNNFFDTVDRFLEARVDRETFTISHGGDPTEVRRYPISIEWPP
                     NALAVQPPVPECRKRVRARLGLAPDVRLGVGVDRLDYTKGILERFAAIERLLELEPRW
                     IGQFSFLQAAAPSRVSIDEYQNLDARVRQVAARINERFGRPGCPPIVLKIEHHDAAQV
                     YEMYRAADLCYVSSLHDGMNLVAKEYVAARDDEQGVLLLSQFTGAARDLAEALIVNPY
                     DIEQSAAAMHLALTMSPEEQRARMRSMRNLVQEFNVYRWAGRMLIDAARMRHRSRVMA
                     RARAARRRSGAL"
     gene            complement(4149..5546)
                     /locus_tag="VIG70_19980"
     CDS             complement(4149..5546)
                     /locus_tag="VIG70_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HEY8252878.1"
                     /translation="MPANIGLIELTGTALFTLAVLHTFSTRYFEHLAHTRTAHAGVWH
                     LLGEVEAVFGFWAFVLVLFMALAYGWGRSSQYLDSLHFTEPMFVFVIMVVAASRPVMQ
                     LARDAVQAAGRVIPAPPAVAGYFLALSLVPLLGSVITEPAAMTLAALMLRERVFAAGI
                     SNRLKYATLGVLFVNVSVGGTLTHFAAPPVLMVAAKWGWTSGFMLSTFGAKALLAVLV
                     NALVVTLLFRRELAARALAPADARPRVPAAFVVLNVLILAAVIFTNHYPEAFMGLFLL
                     FLGLAEAYRRHHERLMLREGLMVAFFLAGLVVLGGQQQWWLQDVLSSLDETALFYGAT
                     ALTAITDNAALTYLGSLVEGVSDEYKYSLVAGAVAGGGLTVIANAPNPAGFAILKDHF
                     EERSISALGLFAAALAPTLVAVICFRGIQGPVHEILDWALWAQWFAGLDPSFLFLLIL
                     PFVVAVVGLWSSRRM"
     gene            5654..7189
                     /locus_tag="VIG70_19985"
     CDS             5654..7189
                     /locus_tag="VIG70_19985"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8252879.1"
                     /translation="MKVLRYLRDAAVFAPCYLLLDWVSYIHPLGAFNITPWNPQPALA
                     IAWMLLGGLGHAPAVFLTIILADGVIRNFPAGYGVTALASLALTAGYGAIAWALREHL
                     RPHADLRSLRQLTLFVVVVAAGTVVVAAGFVGVLHAAGALAPGTALSGWLRFWVGDAV
                     GVLVTAPLLLFASDAQRRSELRALARRGETYVQVGLVAATLWLIFTGLGGDPSHHFYL
                     LFLPLIWIAIRSGMAGAIIAIAIVQAGVVLGIHHRALQTLPTIELQALVAALTLTALY
                     LGVTVEERERAAESLKQSLRLAAAGEMAGAIAHEVNQPLTALANYGRAGQMLLEAGRH
                     AELAGVIGHMLAEAQRAAEVVRRLRDLFRQGTTRLERVTVAELLEAARRAGTGRLSVA
                     AADHLPDVWVDRLQIELVLRNLVANAAEAGGAHAAGEVAITAERHDARHLRIVVSDNG
                     PGIGPAQVRRVFEPFVSGKPSGMGLGLAVSRAIAEAHGGTLEARPASHGEFHLVLPFH
                     SHG"
     gene            7182..7802
                     /locus_tag="VIG70_19990"
     CDS             7182..7802
                     /locus_tag="VIG70_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8252880.1"
                     /translation="MAEPGRAPLVVYVVDDDPSIRDSLALMLGLGGYATRLFADAESF
                     LVAFDAGWSGCVVADLRLPGMSGVELQSRVRSRGSAIPFVIITAHGDVPAARAAFRAQ
                     AVDFIEKPFDDAQLRAGIDTAFALEQRRIDAAELRRADVEKLSRLTAREREVLDQAVK
                     GLHAKEIAAVLGISPRTVEVHRTRIMEKLEVRNIAELVRFAVAAER"
     gene            complement(7813..9000)
                     /gene="rmuC"
                     /locus_tag="VIG70_19995"
     CDS             complement(7813..9000)
                     /gene="rmuC"
                     /locus_tag="VIG70_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY8252881.1"
                     /translation="MVEILLVLTLIAAISTAGLLSIHLMRAKSAEREAREELSRGLAT
                     FSQTLSTQMGATATVQNAQFETLRGAVEQRLERIQQDNASKLEQMRQTVDEKLHATLE
                     KRLSESFKQVSERLEQVHKGLGEMQTLAAGVGDLKRVLTNVKTRGVFGETQLAALLEQ
                     VMAPEQYERNVATRPGSNARVEFAIKLPGRDGGAAVLLPIDAKFPTEDYQRLQAAQEA
                     GDKAALEMAEKALETRVKLEAKAIAEKYVEPPHTTDFALLYLPFEGLFGEVLRRPGLF
                     DHIQRQFRVTICGPTNLLAYLNSLQMGFRTLAIQERSSEVWKVLGTVKSEFGKFAEVL
                     ANTKRQLQTVANTIDQAEVRTRQIERKLKDVEAVPGIEATQENLQLEEDRPLGDLVRL
                     PKA"
BASE COUNT         1370 a         3127 c         3137 g         1402 t
ORIGIN      
        1 cgcgcgctgc cgagcacggt gccgcccgac tgcacgatgc cgccgacgtc gcgcgcggcg
       61 agcggctcga tcaggtcgcc gagcaggccg gcgtagccgt tgcgcacgcc gaagacttcc
      121 cagccgcggg cgagcgcccc ccgcgtcacg gcgcgcaccg cggcgttcat gcccggcgca
      181 tcgccgccgc tcgtcaggac tgcaatcttt ttcaacacga agattctaga gtggaaggct
      241 ctagagcggc catagcatcg cagcgccgcg cacgccgctc gcctcgccgt gccgcgcgcg
      301 cacgatcggg gtcctggggt cttcgcaata gacgtacttc ttcagcagcg gcggcacgtt
      361 ggcgtacagc cgctcgtgct tggaaaggcc gccgccgacc acgattacgt cgggatcgag
      421 cacgttgatg atcatggaga acgcgcgcgc caggcgatcc tcgtagcgcg ccatcgctcg
      481 ctcctcgccg atctcgtcgc gctcccggtt cttcgccggg ccggagagga actgctcgat
      541 gcagccgagc cggccgcagg tgcagcgcgg gcctgggtgc tcttcgtcgc tcatccaggg
      601 aagcggcgtg tggccccatt cgccggcaat gccgtgccgc ccctcgagca tgcgcccgcc
      661 cacgacgatg ccgccgccca cgccggtgcc gaggatcgcg ccgaacacca cgcgcgcgcc
      721 gcgcgcctcg ccgtccaccg cctcggacag cgcgaagcaa ttggcatcgt tgccaaaccg
      781 cacttcgcgc cccaggcgtt cctcaagctg tttcttcaaa ggtttcttgt tgtagggcgt
      841 gttgtaggcg ttctcgaccc ggccggtgtc ggggtcgatc agtcccggca tgccgacgcc
      901 gacgctgcag cgcgcaccgg cgtctttctc cgcctgaaga acctctttcg cgaggagctc
      961 gagagccgtg ccgtactcgc ggctcggata ctcgacgcgg cggcgaagcc gctcggcgcc
     1021 accggcgtct agaaccgcga ttgcgatctt cgtcccgcct acgtcgacgc cgagccgcat
     1081 ttctccagcc aggcttgcag ccaggctctg gccgcggcgg gattttccag ccgccagcgc
     1141 gccgcgctcg ggccctcgcc gaccttcagc gagtggccgc cgaggcggtt gacgacgcga
     1201 aagccgtact cgtcggtgac gtcgtcgccc aagaacaccg ggacgcggcc ggcgaacggc
     1261 ggctcctgca tgaagctctc gatcgccttg cccttgtcgc atcccgcggg cttgagctcg
     1321 accaccatct tgccgccctg cacttcgacc tggtcgccga ggggctcggc ggcctcgcgc
     1381 acagcctttt gcgcggcggc cgcgagctgc ggcgcgaggc ggtagtgcag cgcgacgctt
     1441 gcgcccttgt cttcgaacac caggccctgg tgccgcgcgg cgaagctgcg gatgccgccc
     1501 gcggcgggcc tgagcgcggt caccgggaag cggtgccgat ggcgcgtgcc cattgcgtcg
     1561 cgccgctctg cgccgtgctg cccggccgcg gcgaggatga gcggcgagaa cagctggtcg
     1621 atgcgcgaga gcgaacggcc gctgatgagc gccacggcgc cgccgcacgc gttcttgagc
     1681 cggccgagaa gaatgatctc cggctctccg ggatcgaccg cttcgggcgt gtcggcgatt
     1741 tcgagcagcg tgccgtcgag atcgaggaag aacgcccagt tacggtcgaa tgggggcatc
     1801 ttttttcaca gtgcacccga gcgccgccgc gccgcgcgcg cccgcgccat gacgcggctg
     1861 cggtggcgca tgcgcgcggc gtcgatcagc atgcgcccgg cccagcggta gacgttgaac
     1921 tcctgcacca ggtttcgcat gctgcgcatg cgcgcgcgct gttcctccgg cgacatggtg
     1981 agcgcgaggt gcatcgccgc ggcgctctgc tcgatgtcgt aggggttcac gatcagggcc
     2041 tcggccaggt cgcgcgcggc gccggtgaac tgcgagagca gcagcacgcc ctgctcgtcg
     2101 tcgcgcgcgg cgacgtactc cttcgccacc aggttcatgc cgtcgtgcag gctcgagacg
     2161 tagcacaggt ccgcggcgcg atacatctcg tagacctgcg ccgcgtcgtg gtgctcgatc
     2221 ttgagcacga tcggcgggca gccgggccgg ccgaaacgct cgttgatgcg cgccgccacc
     2281 tgccgcaccc gtgcgtcgag gttctggtac tcgtcgatcg agacgcgcga cggcgccgcc
     2341 gcctggagga aggagaactg gccgatccag cgcggctcga gctcgagcag ccgctcgatg
     2401 gcggcgaagc gctcgaggat ccccttggtg tagtcgagcc ggtcgacgcc cacgccgagg
     2461 cgcacgtcgg gcgcaaggcc gaggcgcgcg cgcacgcgct tgcggcattc gggcaccggc
     2521 ggctggaccg cgagcgcatt cggcggccac tcgatcgaga tgggatagcg gcgcacttcg
     2581 gtcggatcgc cgccgtgcga gatggtgaag gtctcgcggt cgacgcgcgc ctcgaggaag
     2641 cggtcgacgg tgtcgaagaa gttgttgcag tggaactggg tgtggaaacc caggatcgag
     2701 gagccgagca ttccctccag cacctcctcg cgccacgggc agatgccgaa cgcctcggga
     2761 ttgggccagg ggatgtgcca gaaagtgatg atggtggcgc gcggcagccg ctcgcgcacc
     2821 attttcggca gcagggcgaa gtgatagtcc tgcaccagca cgatcgggtc gtcggtgcgc
     2881 gcctcgtccg cgaccacgtc ggcgaagcgc cggttcaccg tctggtaatg ctcccagtcg
     2941 ggggcgcgga agatcggccg cgtgtgcgcg atatggcaga gcggccacag gccttcgttc
     3001 gcgaagccga agtagtagcc ttgctcttct tcctgcgaca gccacacgcg ccggatgcgg
     3061 tacgacggct tctcgggcgg caccatgacg tggtcgtgct tgtcgaccgt gtcgcggtcg
     3121 gccgagcccg cgccgtgcgc gatccaggtg ccggagcatg cgcgcatcac cggctcgagc
     3181 gcggtgacca gcccgctcgc cgggcgctgg atctcgatca cgttgtcctt gcgccgcacg
     3241 tgcaggtaag gctcgcgatt ggaaacgatg agcacctcgt cgcccttcag gtcctggcgc
     3301 aggatgcgcc gcagcgcatc gggcccccag gtggtctggc tctcgtcgcg cgagcgccgc
     3361 tcgctctcga ggtcgctcac cagcgcctgc aggtcgcgcg caatgggacg caactcgggc
     3421 cgcagcccgg ccgccgcggc cttggcggcc ggcgagcgca gcaggtagtc gcctgagagc
     3481 accgccttga tgccgttgac ccagccgcgc cagctgatct cggcgatcac cacggtgatg
     3541 agcgccacca cggcggcaat cccggcgaag agccacagca cgtacttgcg cgtgtcctcg
     3601 ctgcgccgct gggcgaagct catgtcgtgc acgatcatca gttcgccgag cacctgccca
     3661 tccgccgcca ccggctgcgc cgagacgtgc agcgcgccgt cggtcagctc gagcacgaaa
     3721 ttgcgcggct cgcggcgctc ggccttggcg cagcgcaccg cctcgggaaa gacgcgcgtg
     3781 gcgtagagca gcttgcctgc gggatcgcag aagccgagcg cgaaaacccg ctggccctcg
     3841 gcgatccggt cgaagtagcg cagcacgcgc tcgcgcgcgc cgcggccgct gcgcagcagc
     3901 tcgaccagcg ggtcctgcgc cgtctgcacg atcagcgcgc cgcgcatgtc gaggtcgcgc
     3961 acgaaccact ggaaggtcag ccggtcgacc agcggaacga cggcataggc gatcgcggcc
     4021 agcgccaggg ccagcggtac gacaaagcgc agcgaaagcg gcaatcgtcc tccgatcgct
     4081 tgattaaacc cccacgcccc tcagcaaatt ttacgccgcg gcgggggctt ttgaatgtcg
     4141 ctattcgact acatgcgccg gctggaccat agtccgacga ccgcaacgac gaagggcagg
     4201 atcaggagga aaaggaagga agggtcgagc ccggcgaacc actgtgccca gagcgcccaa
     4261 tcgaggatct cgtgaaccgg cccctggatc ccccggaagc aaatgaccgc cacgagcgtc
     4321 ggggcgaggg cggccgcgaa cagcccgagc gcgctgatcg agcgctcctc gaaatggtcc
     4381 ttgaggatgg cgaaaccggc ggggttgggg gcgttggcga tgaccgtcag gccgccgccg
     4441 gcgaccgcgc ccgccacgag cgagtacttg tattcgtccg acacgccctc gaccagcgag
     4501 ccgaggtagg tgagcgcggc gttatcggtg atggcggtga gcgcggtcgc accgtagaac
     4561 agcgcggtct cgtcgaggct ggaaagcacg tcttgcagcc accactgctg ctgtccgcca
     4621 agcaccacga ggccggcgag gaagaatgcg accatcagcc cctcgcgcag catgaggcgc
     4681 tcgtggtggc gccggtaggc ttcggccagc ccgaggaaca ggaggaacag gcccatgaac
     4741 gcttccgggt aatgattggt gaagatgacc gcggcgagga tcagcacgtt cagcacgacg
     4801 aacgccgccg gcacacgcgg ccgtgcatcg gcgggcgcca gcgcgcgcgc tgccagctcg
     4861 cgccggaaga gcagcgttac gacgagcgca ttgaccagca cggcgaggag cgcttttgcg
     4921 ccgaacgtgg acagcatgaa gccgctcgtc cagccccact tcgcggcgac catcagcacc
     4981 ggcggggcgg cgaaatgcgt cagcgtgccg ccgaccgaga cgttcacgaa cagcacgccg
     5041 agcgtggcgt atttcagccg gttggagatg ccggccgcga atacgcgctc gcgcagcatc
     5101 agcgcggcga gcgtcatggc cgccggctcc gtaatgaccg agccgagcag cggcaccagc
     5161 gacaacgcga ggaaatagcc ggcgaccgcc ggcggcgccg ggatcacgcg cccggcggcc
     5221 tgcaccgcgt cgcgcgccag ctgcatcacc ggccggctcg cggcgaccac catgatcacg
     5281 aagacgaaca tcggctcggt gaagtgcagg ctgtcgaggt attgggacga gcggccccag
     5341 ccgtaggcga gcgccatgaa cagcaccagc acgaaagccc agaagccgaa cactgcctcg
     5401 acctcgccga gcagatgcca gacgccggcg tgggcggtgc gcgtgtgcgc gagatgctcg
     5461 aagtaccgcg tggaaaacgt gtgcaggacg gcgagggtga acagcgccgt gccggtgagc
     5521 tcgatcagac cgatgtttgc aggcatcggc ccaaggtacc ggacgccccg agcgcgggat
     5581 attcgggcgt gcgcgtaagc gaaactacgt acttgccccg gcgtccgggc gctcgcacga
     5641 tggcggttcg gcgatgaagg tccttcgata tctgcgcgac gcggcggtgt tcgcgccctg
     5701 ctacctcctg ctcgactggg tgagctacat ccatccgctc ggggccttca acatcacgcc
     5761 gtggaacccg caacccgcgc tcgccatcgc ctggatgctg ctcggcggcc tcggacatgc
     5821 gccggcggtt ttcctgacga tcatcctcgc cgacggcgtg atccgcaatt ttcctgcggg
     5881 gtacggcgtg accgcgcttg cctcgctcgc gctgaccgcc ggctatgggg cgatcgcgtg
     5941 ggcgctgcgc gagcatctgc gcccgcacgc cgacctgcgc agcctgcggc agctcacctt
     6001 gttcgtggtc gtcgtcgccg cgggtacggt cgtggtcgcg gcggggttcg tcggcgtgct
     6061 gcacgccgcc ggcgcgctcg cgcccggcac ggcgttgtcc gggtggctgc gcttctgggt
     6121 cggcgacgcg gtcggcgtgc tggtcaccgc gccgctattg ctctttgcgt cggatgccca
     6181 acgccgaagc gagctgcgcg cgctcgcgcg acgcggtgag acctacgtgc aggtcgggct
     6241 cgtcgccgcc accctgtggc tgatcttcac cggcctgggc ggcgaccctt cgcaccattt
     6301 ctacctgctc ttcctgccgc tcatctggat cgctatccgc agcggaatgg cgggagcgat
     6361 catcgccatc gctatcgtcc aggccggggt cgttctgggc atccaccacc gcgccctcca
     6421 gaccctgccg accatcgagc ttcaggcgct ggtcgccgcg ctcacgctga ccgctctgta
     6481 tctcggggta acggtcgagg agcgcgagcg cgcggccgag agcctcaagc agtcgctgcg
     6541 cctcgcggcc gcaggagaga tggcgggcgc gatcgcgcac gaggtgaacc agccgctcac
     6601 cgcgctcgcc aactacggcc gcgcggggca gatgctgctc gaggccggcc ggcatgccga
     6661 gcttgccggc gtgatcggcc acatgctcgc ggaggcgcag cgcgccgccg aggtggtgcg
     6721 gcgactgcgc gatcttttcc ggcagggaac cacgcgcctc gagcgggtaa cggtcgccga
     6781 gctcctcgag gcggcgcggc gggccgggac gggtcgcctg agcgtggccg ccgcggacca
     6841 cctgcccgac gtgtgggtgg accggctgca gatcgagctg gtgctgcgca acctcgtggc
     6901 caacgccgcg gaagcgggtg gggcccacgc agccggcgag gttgcgataa ccgcggagcg
     6961 ccacgacgcg cgccacctgc gcatcgtcgt ctcggacaac gggcccggga taggcccggc
     7021 ccaggtcagg agagtgttcg agcccttcgt gtccggaaaa ccgagcggca tggggctggg
     7081 gctggcggta agccgggcaa tcgccgaggc gcacggcgga accctcgaag cgcgccccgc
     7141 ctcccacggc gagttccacc tcgtgctgcc cttccattcc catggctgag cccggccgcg
     7201 ccccgctcgt ggtgtacgtg gtcgacgacg atccgtcgat ccgcgattcg ctcgccctga
     7261 tgctgggcct cggcggctac gcgacgcgtc tcttcgcgga cgcggaaagc ttcctcgtgg
     7321 cgttcgatgc cggctggtcg ggctgcgtgg tcgccgacct gcggctgccg gggatgagcg
     7381 gcgtcgagct tcagtcgcgc gtgcgcagcc ggggcagcgc gatcccgttc gtcatcatca
     7441 ccgcgcacgg cgacgtgcct gcggcgcgag cggcgttccg ggcgcaagcg gtcgatttca
     7501 tcgagaagcc cttcgacgac gcgcagctgc gcgcggggat cgatacggca ttcgcgctgg
     7561 aacagcgccg aatcgacgct gccgagctac ggcgcgccga tgtcgagaag ctgtcgcggc
     7621 tcacggcgcg cgagcgcgag gtgctcgatc aggccgtcaa gggattgcac gcgaaggaga
     7681 tcgccgcagt gctcggcatc agcccccgca ccgtggaagt gcaccgcacg cgaatcatgg
     7741 agaagctcga ggtgcgcaac atcgcggagc tggtgcgctt cgcggtcgcg gcggagcgtt
     7801 agccgcggcg gactacgcct tcggcaggcg gacgagatcg ccgagcggcc tgtcttcctc
     7861 gagctgaaga ttttcctgcg tggcctcgat gcccggcacc gcctcgacgt ccttcagttt
     7921 gcgctcgatc tggcgcgtgc gcacttccgc ctggtcgatg gtgttcgcca ccgtctggag
     7981 ctgccgcttc gtgttcgcca gcacctcggc gaacttgccg aactccgact tcaccgtgcc
     8041 gagcaccttc catacctccg aggagcgctc ctggatcgcg agcgtgcgga agcccatctg
     8101 caggctgttc aggtaagcga gcaggttggt cgggccgcag atcgtcacgc ggaactggcg
     8161 ctggatgtgg tcgaacaggc ccggccggcg cagcacttcg ccgaacaggc cctcgaaggg
     8221 caggtagagc agcgcgaagt cggtggtgtg cgggggctcg acgtatttct cggcgatcgc
     8281 cttggcctcg agcttgacgc gcgtctcgag cgccttctcg gccatctcga gcgctgcctt
     8341 gtcgccggct tcctgcgcgg cctgcagccg ctggtagtcc tcggtgggga atttggcgtc
     8401 gatcggcagc agcaccgctg cgccgccgtc ccggcccggc agcttgatgg caaattccac
     8461 ccgggcgttc gagcccggcc gcgtcgccac gttcctttcg tactgctccg gcgccatcac
     8521 ctgctcgagc agcgcggcga gctgcgtctc gccgaacacg ccgcgcgtct tcacgttggt
     8581 cagcacgcgc ttcagatcgc cgacgcccgc ggccagcgtc tgcatctcgc ccaggccctt
     8641 gtgcacctgc tcgagccgct cggacacctg cttgaacgac tcggacaggc gcttctcgag
     8701 cgtggcgtgc agcttctcgt ccaccgtctg gcgcatctgc tcgagcttgg aggcgttgtc
     8761 ctgctggatg cgctccaggc gctgctcgac cgcgccgcgc agcgtctcga actgcgcgtt
     8821 ctgcaccgtg gcggtcgccc ccatctgggt gctgagcgtc tggctgaagg tcgccaggcc
     8881 gcgcgaaagc tcttcacgcg cttcgcgctc ggcgctcttc gcgcgcatca ggtggatgct
     8941 gagcaagccg gcagtcgaga tcgcagcgat cagcgtcagc accagaagaa tctcgaccat
     9001 gcggatagac tactggataa gcttccagta tggaag
//
LOCUS       DATBSZ010000306         3063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1089408, whole genome shotgun sequence.
ACCESSION   DATBSZ010000306 DATBSZ010000000
VERSION     DATBSZ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089408"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VIG70_20000"
     CDS             complement(<1..523)
                     /locus_tag="VIG70_20000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8252882.1"
                     /translation="MKRDLVKGFTYIGVLFVVAMMATGLALVGEVWHTSSLREKEAEL
                     LHIGNEYRKAIERYYVSGKGQYPRELADLVKDPRHPGTVRHLRRLYPDPITGKEEWGL
                     VKSPDGGFAGVYSLSEEAPLKTAGFAVRDASFEGKAKYAEWQFVFAPPAAPAAKPGAK
                     PGAEAKPGAVPTPP"
     gene            complement(520..897)
                     /locus_tag="VIG70_20005"
     CDS             complement(520..897)
                     /locus_tag="VIG70_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8252883.1"
                     /translation="MVKRLFGFTLIELIIVMAIIATLLTIAVPRYYASLDRSKEAVLK
                     ENLYQMRDAIGKYYADKGKYPTTLDALASDKYLRKVPVDPITESAESWVAVPPQDPEK
                     GGVFDVKSGAQGKALDGTEYSAW"
     gene            complement(891..1361)
                     /locus_tag="VIG70_20010"
     CDS             complement(891..1361)
                     /locus_tag="VIG70_20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8252884.1"
                     /translation="MAAKGFTLIELVVAVAIVSILAAAALPMNELVVQRAKEQDLRRA
                     LRDIRTAIDAHKQASDEGRIVKKVGESGYPKRLEELAEGVEDQKSPKRDKIYFLRRIP
                     RDPLATDSALSDAATWAKRSYASPPDDPREGEDVFDVYTTATGKGINGRPYREW"
     gene            complement(1364..1888)
                     /locus_tag="VIG70_20015"
     CDS             complement(1364..1888)
                     /locus_tag="VIG70_20015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252885.1"
                     /translation="MRRRLQRFLDALGPAGVAGVGVLLFCLAFWYTALAPAQAEVKRR
                     TAVAERQARLPDQAQPEPGDHAAELERFYSRFPKIDRLPVELNALYAHAKVSKVQLLQ
                     GEYRMESKGGGLSAYHVTLPVRGSYPQLRQFVGRVLKEMPTTSLDAVRFERKKAADAQ
                     LEAQVRLTIYLRKT"
     gene            complement(1911..2432)
                     /locus_tag="VIG70_20020"
     CDS             complement(1911..2432)
                     /locus_tag="VIG70_20020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252886.1"
                     /translation="MTRAALQLDYVRARRQARVLGVAVLAAALGLAGWLVLEHREAQQ
                     RLQALEASASLLSGARPAPAIPRDRIEAEMKSAQATVRQLALPWAQLIDSLERASIKD
                     VALLNIQPDAQARVLRLTAEARREELMLDYLKRLGATGSFVEVHLVSHQVREDDPQHP
                     IQFSVQATFRNPQ"
     gene            complement(2429..>3063)
                     /locus_tag="VIG70_20025"
     CDS             complement(2429..>3063)
                     /locus_tag="VIG70_20025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252887.1"
                     /translation="RSTVSRLWAERVVMTLSPAQDSAGEQEAWRGALEDLARKVAALR
                     GRVDVTVVLSNHFVRYAVLPEQDGGATAEEELALARFQFAKIHGDRAKAWDVRVSPEG
                     EGAQIACAIDAALLEGIRACFAKNRKARLVSVQPFLMAAFNQSRGKIPGEGAWLLLAE
                     RERTCLARVAARGWASVHNGRETDAEELLERERSRSSGERLPALVLRHQL"
BASE COUNT          421 a         1103 c         1029 g          510 t
ORIGIN      
        1 ccggcggggt ggggactgct cccggcttcg cctcggcgcc gggcttcgcg ccgggtttcg
       61 cggccggggc ggccggcggg gcgaagacga actgccactc ggcgtatttc gccttgccct
      121 cgaagctcgc gtcgcgcacg gcgaagccgg cggtcttcag cggcgcctct tccgacaggc
      181 tgtagacgcc ggcgaagccg ccgtcgggcg acttcaccag cccccactct tccttgccgg
      241 tgatcgggtc gggatagagc cgccgcaggt ggcgcacggt gccggggtgg cgcgggtcct
      301 tgacgaggtc ggcgagctcc ctggggtact ggcccttgcc cgagacgtag tagcgctcga
      361 tcgccttgcg gtactcgttg ccgatgtgga gcagctcggc ctccttctcg cgcaggctgc
      421 tcgtgtgcca cacctcgccg acgagcgcga ggccggtcgc catcatcgcc accacgaaca
      481 gcacgccgat gtaggtgaag cccttcacca ggtcgcgctt caccacgccg aatattccgt
      541 gccgtcgagc gctttgcctt gcgcgccgct cttcacgtcg aacacgccgc ccttctccgg
      601 gtcctgcggc ggcaccgcga cccagctctc cgcgctctcg gtgatcgggt cgaccggcac
      661 cttgcgcagg tacttgtcgc tcgccagcgc gtcgagcgtc gtcgggtact tgcccttgtc
      721 ggcgtagtac ttcccgatcg catcgcgcat ctggtagagg ttctccttga gcaccgcttc
      781 cttcgagcgg tcgaggctcg cgtagtagcg cggcaccgcg atcgtcagca gcgtggcgat
      841 gatcgccatc acgatgatca gctcgatcag ggtaaagccg aagagtcttt tcaccattcg
      901 cggtacggcc gcccgttgat gcccttgccg gtggcggtgg tgtagacgtc gaacacgtcc
      961 tcgccctcgc gcgggtcgtc gggcgggctc gcgtagctgc gcttcgccca cgtggcggcg
     1021 tcggaaagcg cgctgtcggt ggcaagcgga tccctgggta tccgccgcag gaagtagatc
     1081 ttgtcgcgct tcgggctctt ctggtcctcg acgccctcgg cgagctcctc cagccgcttc
     1141 ggatatccgg actcgcccac tttcttgacg atgcgcccct cgtccgaagc ctgcttgtgc
     1201 gcgtcgatcg cggtgcggat gtcgcgcagc gcgcgccgca ggtcctgctc cttcgcgcgc
     1261 tgcaccacga gctcgttcat cggcagcgcc gccgcggcga ggatcgacac gatcgcgacc
     1321 gcgactacca gctcgatgag agtgaatccc ttcgccgcca ttcctaggtt ttcctcaggt
     1381 agatggtgag gcgcacctgc gcttcgagct gcgcatcggc ggccttcttg cgctcgaagc
     1441 gcaccgcgtc gagcgaggtg gtcggcattt ccttcagcac gcggccgacg aactgccgca
     1501 gctgcgggta gctcccgcgc accggcagcg tgacgtggta ggcgctgagt cctccgccct
     1561 tcgactccat ccggtattcg ccctgcagga gctggacctt cgacaccttg gcgtgcgcgt
     1621 acagggcatt gagctcgacc ggaagccggt cgatcttcgg aaagcggctg tagaagcgct
     1681 cgagctccgc ggcgtggtcg ccgggctccg gctgcgcctg gtcgggaagc cgcgcctggc
     1741 gctcggcgac cgccgtgcgc cgcttgacct cggcctgcgc cggcgcaagc gcggtatacc
     1801 agaacgccag gcagaagagc agcacgccca cgccggcgac gcccgccggg ccgagcgcat
     1861 cgaggaaccg ctgcaggcgc cgcctcatgt cgcgcgtctc ctcattccac tcattgaggg
     1921 ttccggaagg tcgcctgcac cgagaactgg atcgggtgct gcggatcgtc ctcgcgcacc
     1981 tgatggctca cgagatgcac ctcgacgaag ctgccggtcg cgcccaggcg cttcaggtag
     2041 tcgagcatca gctcctcccg gcgcgcctcc gcggtcaggc gcagcacgcg agcctgcgcg
     2101 tcgggctgga tgttcagcag ggcgacatcc ttgatcgagg cgcgctcgag cgagtcgatg
     2161 agctgcgccc aggggagcgc gagctggcgc accgtggcct gcgcactttt catctcggct
     2221 tcgatccggt cgcgcgggat cgcgggcgcc ggccgggccc cgctcaacag cgacgcggac
     2281 gcctcgagcg cctgcagacg ctgctgcgcc tcgcggtgtt cgagcaccag ccagccggcg
     2341 aggcccaggg cggcggcgag gacggccacg ccgagcaccc gggcttgccg tcgcgcgcgg
     2401 acgtaatcga gctgcagcgc ggcgcgcgtc acagttgatg cctcaggacg agcgcaggca
     2461 gccgctcgcc gctcgaccgg ctgcgctcgc gctcgagcag ctcctccgcg tcggtctcgc
     2521 ggccgttgtg caccgaggcc cagcctcgcg cagccactcg cgcgaggcag gtgcgctcgc
     2581 gctcggcaag gagcagccag gcgccttcgc cggggatctt cccgcgcgac tggttgaacg
     2641 ccgccatgag aaagggctgg accgacacca ggcgcgcttt ccggttcttg gcaaaacagg
     2701 ccctgatgcc ctcgaggagc gcggcgtcga tcgcgcaggc gatctgcgcg ccttcgccct
     2761 cgggcgagac gcgcacgtcc caggccttcg cacgatcgcc gtggatcttg gcgaactgga
     2821 agcgcgcgag cgcgagctcc tcctccgcgg tcgcgccgcc gtcctgctcc ggcagcacgg
     2881 cgtagcgcac gaaatggttc gacagcacca cggtgacgtc gacccgcccg cgcaacgcgg
     2941 ccaccttgcg cgccagatcc tcgagcgcgc cgcgccatgc ctcctgctcg cccgcggaat
     3001 cctgcgcagg cgaaagcgtc atcacgaccc gctcagccca caaacgtgac acggttgatc
     3061 tcc
//
LOCUS       DATBSZ010000307        14177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1021334, whole genome shotgun sequence.
ACCESSION   DATBSZ010000307 DATBSZ010000000
VERSION     DATBSZ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14177
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021334"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VIG70_20030"
     CDS             complement(<1..281)
                     /locus_tag="VIG70_20030"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189552.1"
                     /note="Converts isocitrate to alpha ketoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY8252888.1"
                     /translation="MAYQHIKIPSGGDKIKVNKDFSLSVPDKPIIPYIEGDGTGVDIT
                     PVMLKVVDAAVKKAYGGKKKIVWMEMYAGEKSCKVYGENVWLPDETLAAA"
     gene            533..805
                     /locus_tag="VIG70_20035"
     CDS             533..805
                     /locus_tag="VIG70_20035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252889.1"
                     /translation="MNKLLSVIVAAMFAAVSASAFAASHAGAQTKDEKKTEKKAAPKG
                     DAKAAPKKDAKAAPKKDAKAAPKKDAKAAPKKDAKKAPAKKDEMKK"
     gene            857..1279
                     /locus_tag="VIG70_20040"
     CDS             857..1279
                     /locus_tag="VIG70_20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY8252890.1"
                     /translation="MRLLVSFMLCAAPALAQELPVVQLSAGMHLIRAEVAADFASRGR
                     GLMHRKSLGANAGMLFVFDQAAIHCMWMKNTYVPLSVAFLDEHGTIVNIADMQPHSEQ
                     SHCATRPSVYALEMAQGWFAQRGIKPGAKLGGLDKLPR"
     gene            complement(1528..2124)
                     /locus_tag="VIG70_20045"
     CDS             complement(1528..2124)
                     /locus_tag="VIG70_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY8252891.1"
                     /translation="MGALFQSLGRTITAGVVLLIIFIILAGLGTGSWVRAGDHAWWAF
                     FMRWLHVASGVMWIGLLWYFNFVQTPSMPKIPDEQKPAISKVIAPTALFWFRWGAMAT
                     IVTGLLLAFMNGYVGQALSLQKGYHAIGLGMWLGAIMWFNVWFIIWPNQKKALGIVQV
                     APEQKAAAAKMAGMTSRFNTMLSIPMLYCMVAQQNGGL"
     gene            2198..2977
                     /gene="otnI"
                     /locus_tag="VIG70_20050"
     CDS             2198..2977
                     /gene="otnI"
                     /locus_tag="VIG70_20050"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEY8252892.1"
                     /translation="MAKLAANLSMLFPELPFLERFAAAAKAGFRGVEYQYPYDWQPAD
                     IAAAARDARVEVLLHNMPQGDVQRGERGTACLPGREQRFRDDLERAIEYARVVRCPSL
                     HLLAGVAPQGADRAALHATFVSNLKQAATRLAREGVRALIEPLSERTVANCFLRSSRQ
                     AAQVLNDVGADNVFIQYDLFHMQIMEGNLAATIERLLPRIGHMQIADVPGRDEPGNGE
                     INFDFLLRHIDALGYSGWVGCEYNPRGDTLEGLKWARPYLS"
     gene            2953..4410
                     /locus_tag="VIG70_20055"
     CDS             2953..4410
                     /locus_tag="VIG70_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8252893.1"
                     /translation="MGAALPVVRALREFLPERAILHEEEDTRPYECDGLTAYRQLPMV
                     VALPETEEQVRRILQTCSALKIPVVPRGAGTGLSGGALPPGDGVLLSMAKFMRVLRID
                     PLARVAVVQPGVRNAVVSELAAPFGLYYAPDPSSQIACTIGGNVSENSGGVHCLKYGL
                     TLHNVLGVRGFTIDGEPLQLGGEAPDAPGYDLLALAVGSEGMLFVLTEVTLKLLPKPQ
                     SARVIMASFDDVEKAGAAVADVIAAGIVPAGLEMMDRPATQAVEQFVQAGYDLEAAAI
                     LLCESDGTPEEVEDEIARMQTLLARAGATRMTVSRDEAERLRFWSGRKAAFPAVGRIS
                     PDYYCMDGTIPRKRLGEVLRFIAALEKKYGLRCPNVFHAGDGNLHPLILFDANNADEL
                     HRTELFAGEVLEKCVEVGGTITGEHGVGVEKINQMCVQFRPTELELFRAVKRAFDPQG
                     LLNPGKAVPTLARCAEYGRMRVRGGQLPHPELPRF"
     gene            4407..5450
                     /gene="glcE"
                     /locus_tag="VIG70_20060"
     CDS             4407..5450
                     /gene="glcE"
                     /locus_tag="VIG70_20060"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEY8252894.1"
                     /translation="MIESLADSIREAAARHRPLRLRGGGTKDFYGNALRGEVFDTRGY
                     SGIVAYEPTELVVTARCGTSLSELEKRLEANSQCLPFEPPHFGPGATIGGCVAAGLSG
                     PRRASAGALRDFVLGVKLIDGRGQALSFGGQVMKNVAGYDVSRLVAGSLGTLGLIAEV
                     SLKLAPKPQAERTVRLDASQARALELMNRWAGQPLPVSATAWHKGELTVRLSGSGAGV
                     RAATAGIGGAALDEPFWSGIREQSHAFFFGPEPLWRLSVPSTSPPLELQGRQLIEWGG
                     ALRWLKSSEDPKTVRAAAKRCNGHATLFRAPDKTAGCFSPLEPVLARVHGQLKSAFDP
                     AGIFNPGRMYPEA"
     gene            complement(5447..5791)
                     /locus_tag="VIG70_20065"
     CDS             complement(5447..5791)
                     /locus_tag="VIG70_20065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252895.1"
                     /translation="MKALTWLLVIAAIGVLGYALWNWRQRFLERQRAAQERFAAFMAQ
                     AKPAAPAVAADPGLPQQRLLLEAGAKAGEAGEPVLSIQLYARLLARYPESALAAQARA
                     AVETQKQKLAKP"
     gene            5810..6994
                     /gene="glcF"
                     /locus_tag="VIG70_20070"
     CDS             5810..6994
                     /gene="glcF"
                     /locus_tag="VIG70_20070"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY8252896.1"
                     /translation="MQTELADFVRDTPEGREADAILRKCVHCGFCTATCPTYQVLGDD
                     LDSPRGRIYLMKRALEGAPVSEKTRLHLDRCLTCRACETTCPSGVRYGRLVDIGRAYV
                     EKRTRRTAWERLRRAVLSFGLPRTALFTAALRMGQLVGMAPATRPGGAWPAPRHDRKM
                     LVLRGCVQPALAPSINAAAARVLDRIGVSLLEAPGAGCCGGVRFHLNRQEAGRDDMRA
                     LIDAWWPMVAGSEIEAIVMTASGCGVTVKEYGHLLEIDPSYRGKAERVAGLTVDLCEV
                     IGPEDLPQRGKLGRIAFHSPCTLQHGQQLRGRIEALLTRVGYELVPVRDAHLCCGSAG
                     TYSLLQPQIAGELRARKLAALEEGAPQAIATANIGCLVHLQAPTTTPVRHWIELVDAA
                     LA"
     gene            7004..9055
                     /locus_tag="VIG70_20075"
     CDS             7004..9055
                     /locus_tag="VIG70_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEY8252897.1"
                     /translation="MAAVVPAVVPHVVRVACPHDCPDTCAMLVTVENGVATQIRGDPD
                     MPFTEGTLCTKVAHYLERTYAPDRLLYPQKRVGRKGEGKFRRITWDEALDEIAVRLKA
                     LAADNPESILPCSYAGTMGMVQYMSMDRRFFHRLGASLLDRTLCSSAGKFGLKATLGG
                     SVGMDPERFDEARLIILWGANPIVSNLHLWSRVQTAKRRGAKVIAIDPYRSLSAEKCT
                     QHIALLPGTDGALALGMMHVLISEGLLDPEYVAKYTLGYEALKQRVLKEYSPSWAARV
                     CGISTDEIVQLAREYGTAKPAAIRLNYGMQRHAGGGIAARTIACLPALTGAWRDPAGG
                     IVLTTADFYGFDHAALERADLLAGRRPRVINQSKLGDALTGAEPPVRMTIVYNNNPVA
                     VCPDSEKVIAGFSREDLFTVVMDHFQTDTADYADILLPATTQLEHHDVHKSYGHLYML
                     ANNPAIAPLGESLPNSEVFRRLAARMGFDEPCFRDSDEDICRTALKGVDWDRLKQTGW
                     QKLDVPARFAPFAQGGFPTPSGKCEFYSAALEKQGIDPLPFYNPPAEVENRELEKRYP
                     LAFISPPARNFLNSSFANIARFRELEREPHLDLHPKDAAERGIADGDMVRVFNDRGSQ
                     RLRARVNGKPRPGVVVAPSVWWKKYAPDGRNANNLTSQRTSDLGGGATFYDCRVQVER
                     A"
     gene            9052..9825
                     /locus_tag="VIG70_20080"
     CDS             9052..9825
                     /locus_tag="VIG70_20080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8252898.1"
                     /translation="MSKADELRLLRDAIKSFPDALAVWSAEGRLIACSDLYARLAAAT
                     PAGNSAAEYQSADGRWLRRTLRRVEGGGSVELVADVSELRRAEQASRFLGYHDTLTGL
                     PNRRLLDDRLRQAIHLAQRRDRMVATLLVRLEDFRQVNDTLGEGAGDALLREVAQRLS
                     ACVRKADTLARAGTDEFALVVCDLKDGADCRIVADKLLQALAPELRVGGRRLKLAVSV
                     GISLFPSDGGDGEALLRNADAAVLRAKQLGRNQVRFFGR"
     gene            9822..11582
                     /locus_tag="VIG70_20085"
     CDS             9822..11582
                     /locus_tag="VIG70_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY8252899.1"
                     /translation="MKLARFLWPYRMRIAVALLALLVTSACVLALGRGLQHVVDRGFG
                     SGDPRLLDQALAAMIAVVVVFAAATWVRFYFMMSTGERVVTDLRRAVFDHILGLDPAF
                     FEKERTGEVVSRLTNDATLVQQVIGYGLSMFVRNGLMMIGAAAMLFVTSWKLALLVLV
                     GAPATLLPILLLGRRVRGLSRASQDRVADVSAYVDEAIHEIRTVQAYAHEDADRAGFA
                     RHAEAAYAAGVERISYKAFLIAAVILIAFSAVGIILWIGGHDVLAGRLTQGELAAFVF
                     YAAVVAMGAGTVAEVWGELQRAAGATERLVELLDTPPRLVAPAVPVAAPIRSPLGVSF
                     EEVGFAYPARAATPALVRFTLQVAPGERVALVGPSGAGKSTVFALLLRFYDPQQGAVR
                     IDGIDLREMEPGAARRLVAVVPQEPVIFAASVLENVRYGRPDATREAVSAACEQACAL
                     EFIERLPHGLDTPLGERGVTLSGGQKQRLSIARALLADRPILLLDEATSSLDAAAERL
                     VQQGLEALEKGRTTLVIAHRLATVQNAGRIVVLERGEIAAQGSHAELMRQGGLYASLA
                     RLQFLGGRDEKDHRGLRVIG"
     gene            11601..13049
                     /locus_tag="VIG70_20090"
     CDS             11601..13049
                     /locus_tag="VIG70_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8252900.1"
                     /translation="MKWVGMLAAVIACAAQAQTFTVGSKRFTESYILGEILSQTAGAA
                     HKPGLGNTAILLEALKAGAIDLYPEYTGTIAREILKSEERLDLAALNQRLKSLGLAAS
                     VPLGFSNSYALGMRRKDAERLGIKKASDLAGHPQLRFGLSHEFLGRRDGWPGLQSAYG
                     LPHKPRGLDHGIAYEALAAGEIDVMDLYTTDAKIERYAIVALADDRAFFPAYDAVLLY
                     RADIPQRFPAAFDELRKLEKRIDEQTMVRLNARAELDKVAFADVAREYLGAKIESTKG
                     FWAAMFAPDFGRLLAEHLWLVFASLAIAVLVGVPLGLIAAKYGWVAQPVLLFTGVLQT
                     IPSLALLAFLIPVTGTIGVWPALIALFLYALLPITRNTHAGIAGVPRGLVQAGTALGL
                     RSRQILLKVEMPIAMPVIMAGVKTSAVISVGTATIAAFIGAGGFGERISQGLALNDHA
                     VLLAGALPAAALALAVHAAFEIAERSLSRLPR"
     gene            13081..>14177
                     /locus_tag="VIG70_20095"
     CDS             13081..>14177
                     /locus_tag="VIG70_20095"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY8252901.1"
                     /translation="MPQLIGVPREIEAGEKRVATVPDVVEKLIKLGFRVAVQSGAGDA
                     ANFADDTYRAAGAEIIGDAERLWTSADMIFKVRAPSMAEVQLMRDGTTSSTTLVSFIW
                     PAQNPQLMQALAAKKATVLAMDSVPRISRAQKLDALSSMANIAGYRAVIEAAHHFGRF
                     FTGQITAAGKVPPAKVFIIGAGVAGLAAIGAASGLGAIVRANDTRPEVADQIKSLGGE
                     YVGVDYVEEGSGTGGYAKVMSEGFQKAQREVFARQAKEVDVIITTALIPGKPAPRLIT
                     ADMVRSMRPGSVIVDLAAEQGGNCELTEPGQVTVKHGVTIIGYTDLPSRLAKQSSTLY
                     ATNLLRLTEELCKGKDGVINVNMDDEVIRGTT"
BASE COUNT         2245 a         4832 c         4881 g         2219 t
ORIGIN      
        1 gccgcggcga gcgtctcgtc gggcagccac acgttctcgc cgtacacctt gcaggacttc
       61 tcgccggcgt acatctccat ccagacgatc ttcttcttgc cgccgtaggc tttcttcacc
      121 gcggcgtcga ccaccttgag catcacgggc gtgatgtcga cgccggtgcc gtcgccttcg
      181 atatacggaa tgatcggctt gtcggggacc gacagcgaaa agtccttgtt gaccttgatc
      241 ttgtcgccgc cggagggaat cttgatgtgt tggtaggcca tagggggcgc aattataaga
      301 cccccggcgc tgcacccgcg cgcgttcagc ggaggctggc aagaggtgtt tcctatgtat
      361 aaacaacggt ctagaggaca aaaaaagccc tttgccgctt ttgtctagaa tgcgcgccgt
      421 ggggaaacac ccacagggag gtcaaccggt tcggcagtgg cgcgttgttc gcctccgggt
      481 ggccagagga ggttgctcgg ccccgtgtcc aaaaattaaa caggagaagg aaatgaacaa
      541 gctgctgtcc gtgatcgtcg ccgcgatgtt cgcggcggtt agcgcttccg ccttcgcggc
      601 gtcgcacgcc ggcgcacaga cgaaggacga gaagaagacc gagaagaagg cggctccgaa
      661 aggggacgcg aaggccgctc cgaagaaaga cgcgaaagcc gctccgaaga aagacgcgaa
      721 ggcggctccg aagaaagacg cgaaggccgc tccgaagaag gacgccaaga aggcgcccgc
      781 caaaaaagac gaaatgaaga agtaacccgc tgccgaacgc cgaaaaaggc aggacaatgt
      841 cctgcctttt tcatttatgc gtcttctcgt ttccttcatg ctctgcgccg cgccggcgct
      901 ggcgcaggag ctgccggtgg tgcagttgag cgccggcatg catctcatcc gcgccgaggt
      961 ggcggcggac ttcgccagcc gcggccgcgg gctgatgcac cgaaagtccc tcggcgccaa
     1021 cgccgggatg ctgttcgtgt tcgaccaggc ggcgattcac tgcatgtgga tgaagaacac
     1081 ctatgttccg ctgtcggtcg cgttcctcga cgagcacggc acgatcgtca acatcgcgga
     1141 tatgcagccg cacagcgagc agtcccattg cgccacgcgg ccttcggtgt acgcgctcga
     1201 gatggcgcag ggctggttcg cccagcgcgg catcaagccg ggcgcgaagc tcggcgggct
     1261 cgacaagctc cctagataag gatcatcagc gccaggccgg cgatgaccgc cgcgcgcgcg
     1321 agcggcgtcc atagcggccg cgcgacgttg cgcgaggatt tccacaccgc ccagccccag
     1381 gcccagtaga cccccaggcg caggacgtcc agcaggtcac cctggaccgc agaccccgcg
     1441 tcgcgcgctt cctcggcgat cagtaccagg cctgcgatga cgcaggcgac cgccgccccc
     1501 catagccacc agacctgcca aagaagccta gagcccgccg ttttgctggg ccaccatgca
     1561 gtacagcatc gggatcgaga gcatggtgtt gaagcgcgag gtcatgccgg ccatcttggc
     1621 cgccgcggcc ttctgctccg gggccacctg gacgatgccg agcgccttct tctggttcgg
     1681 ccagatgatg aaccagacgt tgaaccacat gatcgcgccg agccacatgc ccagcccgat
     1741 cgcgtggtag cccttctgca gcgacagagc ctggccgacg tagccgttca tgaacgccag
     1801 gaggaggccg gtgacgatcg tcgccatcgc gccccagcgg aaccagaata gcgcggtcgg
     1861 cgcgatcacc ttgctgatcg cgggcttctg ctcgtcgggg atcttgggca tcgagggcgt
     1921 ctgcacgaaa ttgaagtacc agagaagccc gatccacatc acgcccgagg ccacgtgcag
     1981 ccagcgcatg aagaacgccc accaggcgtg gtccccggca cggacccacg agccggtgcc
     2041 gaggccggcg aggatgatga agatgatgag aaggacgacg cctgcggtga tggtgcggcc
     2101 gagcgactgg aagagggcac ccattctttt ctcctgttcg tgtgatgggt ttgcagccaa
     2161 aaaagtctag cacttgtgag cgggttatgc tggcgccgtg gcgaagctgg cggcgaatct
     2221 ttccatgctg tttcccgagt tgccgttcct cgagcgcttt gccgccgcgg cgaaagccgg
     2281 attccgcggc gtcgaatacc agtaccccta tgactggcag cccgcggaca tcgcagcggc
     2341 cgcgcgcgat gcccgcgtcg aggtgctgct gcacaacatg ccgcagggcg acgtgcagcg
     2401 cggcgagcgc ggcacggcct gcctgcccgg gcgcgagcag cgcttccggg acgatctgga
     2461 gcgcgcgatc gaatacgcgc gcgtcgtgcg ctgcccgagc ctgcatctgc tggcgggcgt
     2521 ggccccgcaa ggggcggatc gcgctgcgct gcacgcgacc ttcgtttcga acctgaagca
     2581 ggcagcgacg cgcctcgcgc gcgagggcgt gcgcgcgctg atcgagccgc tgagcgagcg
     2641 caccgtggcc aactgcttcc tgcgctcaag ccggcaggcg gcgcaggtgc tgaacgacgt
     2701 cggcgccgac aatgtcttca tccagtacga cctgttccac atgcaaatca tggaaggcaa
     2761 cctcgccgcg acgatcgagc gcctgctgcc gcgcatcggc cacatgcaga ttgccgacgt
     2821 gccggggcgc gacgagccgg gcaacggcga gatcaacttc gacttcctgc ttcgccacat
     2881 cgacgcgctc gggtactcgg gctgggtggg atgcgaatac aatcccaggg gtgacacgct
     2941 ggaaggcttg aagtgggcgc ggccctacct gtcgtaagag ccctgcgcga gttcctgccg
     3001 gaacgcgcga ttctccacga ggaagaagat acccggccgt acgaatgcga cggcctgacc
     3061 gcgtatcgcc aattgccgat ggtggtcgcc ctgcccgaga ccgaggagca ggtgcggcgc
     3121 atcctccaga cctgctccgc cctgaagatt ccggtggtgc cgcggggggc gggcaccggc
     3181 ctgtccggcg gcgcgctgcc gccgggcgac ggcgtgctgc tttcgatggc gaagttcatg
     3241 cgcgtgctgc gcatcgatcc gctcgcgcgg gtggcggtgg tgcagcccgg cgtgcgcaac
     3301 gcggtggtgt cggagctggc ggcgccgttc ggcctgtact acgcgcccga cccgtcgagc
     3361 cagatcgcct gcaccatcgg cggcaacgtg tcggagaact ccggcggcgt gcattgcctg
     3421 aagtacggcc tcacgctgca caacgtcctc ggcgtgcgcg gcttcacgat cgacggcgag
     3481 ccgctgcagc tcggcggcga agcgcccgac gcgcccggct acgacctgct cgccctcgcg
     3541 gtgggctccg aaggcatgct gttcgtgctc accgaggtga cgttgaagct cttgccgaag
     3601 ccgcagagcg cgcgggtcat catggcgtcg ttcgacgacg tggagaaagc cggggccgcg
     3661 gtcgccgacg tgatcgccgc gggcatcgtt cccgcggggc tggaaatgat ggaccggccg
     3721 gcgacgcagg ccgtggagca gttcgtccag gccggctacg acctggaggc ggcggcgatc
     3781 ctgctgtgcg aatccgacgg cacgcccgag gaagtcgagg acgagatcgc gcgcatgcaa
     3841 acactgctcg cgcgcgccgg ggcgacgcgc atgacggtgt cgcgcgacga agccgagagg
     3901 ctgcggttct ggtctggccg caaggcggcg ttccccgccg tcggccgcat ctcgcccgac
     3961 tactactgca tggacggcac gattccgcgc aagcgcctcg gcgaagtgct ccgcttcatc
     4021 gccgccctgg agaaaaaata cgggttgcgc tgcccgaacg tgttccacgc gggcgacggc
     4081 aacctgcacc cgctgatcct gttcgacgcc aacaacgcgg acgagctcca ccgcacggag
     4141 ctgttcgccg gcgaggtgct cgagaaatgc gtggaggtcg gcggcaccat taccggcgag
     4201 cacggcgtcg gcgtggagaa gatcaaccag atgtgcgtgc agttccggcc gaccgagctc
     4261 gagctgttcc gcgcggtcaa gcgcgccttc gacccgcagg gcctgctcaa cccgggcaag
     4321 gcggtgccca cgctcgcgcg ctgcgcggaa tacgggcgca tgcgcgtccg cggcggtcag
     4381 ctgccacacc cggaactgcc tcgcttttga tcgagtccct tgccgacagc attcgcgagg
     4441 ccgccgcgcg ccatcggccg ctgcgcctgc gcggcggcgg caccaaggac ttttacggca
     4501 atgcgctgcg cggagaggtc ttcgatactc gcggctattc cggcatcgtc gcgtatgagc
     4561 ctaccgagct ggtggtgacg gctcgctgcg gcacttcgct ttcggaactg gaaaaaaggc
     4621 tcgaggcgaa cagccagtgt ctccccttcg agcctcccca cttcggcccc ggggctacga
     4681 tcggcggctg cgttgcggcc ggcttgtccg ggccgcggcg cgccagcgcc ggggcgctgc
     4741 gcgatttcgt gctcggcgta aagctgatcg acggccgcgg ccaggcgctc agcttcggcg
     4801 gccaggtgat gaagaatgtc gccggctacg acgtgtcgcg cctggtggcc ggctcgctcg
     4861 gcacgctcgg cctgattgcc gaggtgtcgc tcaagctcgc gccgaagccg caggcggagc
     4921 gcaccgtgcg cctcgacgcg tcgcaggcgc gcgcgctcga gctcatgaac cgctgggccg
     4981 ggcagccgct tccggtcagc gccacggcgt ggcacaaggg cgagctcacg gtgcggcttt
     5041 cgggctcggg cgccggcgtg cgcgcggcaa cggccgggat cggcggcgct gcgctcgacg
     5101 agcccttctg gagcgggatc cgcgagcaga gccacgcgtt ctttttcggt cccgagcctt
     5161 tgtggcggct ttcggttccg tcaacctcgc cgccgctcga gctgcagggc aggcagctca
     5221 tcgaatgggg cggcgcgttg cgctggctca agtcttcaga agaccccaaa accgttcgcg
     5281 ctgcggcgaa gcgctgcaat ggccacgcga ccttgttccg cgcgccggac aagaccgcgg
     5341 gctgcttctc gccgctcgag ccggtgctcg cgcgggtgca cggccagctg aagagcgcgt
     5401 tcgatccggc cgggatcttc aacccgggcc ggatgtatcc ggaggcctag ggcttggcga
     5461 gcttctgctt ctgcgtctcg acggcggcgc gcgcttgggc ggcgagcgcg ctctccgggt
     5521 atcgcgcgag cagccgcgcg tacagctgga tcgacagcac gggctcgccg gcttctccgg
     5581 ccttcgcccc tgcctccagg aggagccttt gctgcggcaa gcccggatcg gcggcaacgg
     5641 ccggcgcggc gggcttcgcc tgcgccatga aggcggcaaa gcgctcctgt gcggcgcgct
     5701 ggcgctcgag aaagcgctgc cgccagttcc agagcgcgta ccccagcacg ccgatcgcgg
     5761 caatcaccag cagccaggtc agcgctttca tgcgaaaagt atagccgccg tgcagaccga
     5821 gctcgccgac ttcgtccgcg acacgcccga gggccgggag gccgacgcca tcctgcgcaa
     5881 gtgcgtgcac tgcgggttct gcaccgcgac ctgcccgacc taccaggtgc tgggcgacga
     5941 cctcgacagc ccgcgcgggc gcatctacct catgaagcgc gcgctcgaag gcgcgccggt
     6001 gagcgaaaag acccgcctgc acctcgaccg ctgcctgacg tgccgcgcct gcgagaccac
     6061 ctgtccctcg ggcgtgcgct acgggcgcct cgtcgacatc ggccgcgctt acgtcgagaa
     6121 gcgcacgcgg cgcacggcat gggagcggtt gcggcgggcg gtgctttcat tcggcttgcc
     6181 gcgcacggcg ctcttcaccg ccgcgctgcg aatggggcaa ctggtcggga tggctcccgc
     6241 cacgcggccg ggcggcgcct ggccggcccc gcgtcacgac cggaagatgc tcgtgctgcg
     6301 cggctgcgtg cagccggcgc tcgcgccttc gatcaacgcg gcggcggcgc gcgtgctcga
     6361 tcgaatcggc gtgtcgctgc tcgaggctcc cggcgccggc tgctgcggcg gggtccggtt
     6421 ccacctcaat cgccaggaag cggggcgcga cgacatgcgc gcgctgatcg acgcatggtg
     6481 gccgatggtg gcgggcagcg agatcgaggc gatcgtgatg acggcgagcg gctgcggcgt
     6541 gacggtgaag gagtacggcc acctgctcga gatcgatccg agctatcgcg gcaaggccga
     6601 gcgcgtcgcc gggctgaccg tcgacctgtg cgaagtgatc gggcccgagg accttcccca
     6661 gcgcggaaag ctcggccgca tcgcctttca ctcaccgtgc acgctccagc acgggcagca
     6721 gctgcgcggg aggatcgagg ccctgctcac gcgcgtcggc tacgagctcg tgcctgtgcg
     6781 cgatgcgcac ctctgctgcg gctcggcggg cacctattcc ctgctgcagc cgcagatcgc
     6841 cggcgagctg cgcgcgcgca agctcgccgc gctggaagag ggcgcgcccc aggcgatcgc
     6901 gaccgcgaat atcggctgcc tggtgcatct gcaagcgccg acgacgacgc cagtgcgcca
     6961 ctggatcgag ctcgtcgacg ccgcgctggc gtagaatccc cctatggccg cagtcgttcc
     7021 cgccgttgtt cctcacgtcg ttcgcgtcgc ttgtccgcac gattgccccg acacctgcgc
     7081 gatgctcgtc acggtcgaga acggcgtcgc tacccagatc cgcggcgatc cggatatgcc
     7141 cttcaccgag ggcacgctct gcaccaaggt tgcgcattac ctcgagcgca cttatgcgcc
     7201 cgaccggctg ctgtatccgc agaaacgggt tggccggaaa ggcgaaggca agttccggcg
     7261 catcacctgg gatgaagccc tcgacgagat cgcggtgcgg ctgaaggcgc tcgccgccga
     7321 caatcccgag agcatcctgc cctgcagcta cgccggaacc atgggcatgg tgcagtacat
     7381 gtcgatggac cggcgcttct tccacaggct cggcgcctcg ctcctcgacc gcacgctgtg
     7441 ctcgagcgcc gggaagttcg gcctcaaggc aacgctcggc ggctcggtcg gcatggatcc
     7501 ggagcgcttc gacgaggcgc ggctcatcat cctgtggggc gcgaacccga tcgtgtcgaa
     7561 cctgcacctc tggtcgcgcg tgcagacggc gaagcgccgc ggcgccaagg tcatcgcgat
     7621 cgatccgtac cgcagcctgt cggccgagaa gtgcacccag cacatcgcgc ttcttcccgg
     7681 caccgacggc gcgctcgcgc tcggcatgat gcatgtgctg atcagcgaag gcttgctgga
     7741 ccccgaatac gtcgcgaagt acacgttggg ttacgaagcg ctgaagcaac gagtcctcaa
     7801 ggaatattcg ccatcctggg cggctcgcgt gtgcgggatt tctaccgacg aaatcgtgca
     7861 gctcgcgcgc gaatacggca ccgccaagcc ggcggcgatc cggctcaact acggcatgca
     7921 gcggcacgcg ggcggcggca ttgccgcgcg caccatcgcc tgcctgccgg cgctcaccgg
     7981 cgcgtggcgc gatccggccg gcggcatcgt gctcaccacc gcggacttct acggcttcga
     8041 ccacgcggcg ctcgagcgcg ccgacctgct cgccggacgc cggccgcgcg tgatcaacca
     8101 gtcgaagctc ggcgacgcgc tgaccggcgc cgagccgccg gtgcgcatga cgatcgtcta
     8161 caacaacaac ccggtcgcgg tatgccccga ctccgagaag gtgatcgcgg gattctcgcg
     8221 cgaggatctg ttcacggtgg tgatggatca cttccagacc gacaccgccg attacgccga
     8281 catcctgctg ccggcgacga cgcagctcga gcaccacgac gtgcacaagt cctatgggca
     8341 cctctacatg ctcgccaaca atcccgcgat cgcgccgctc ggcgagtcgc tgcccaattc
     8401 cgaggtgttc cgccgccttg cggcacgcat gggcttcgac gagccctgct tccgcgactc
     8461 tgacgaagac atctgccgca ccgccctgaa gggcgtcgac tgggaccgcc tcaagcaaac
     8521 cggctggcag aagctcgacg ttcccgcgcg cttcgcaccg ttcgcgcagg gcggcttccc
     8581 gactccgtcc ggcaagtgcg agttctacag cgcggcgctg gaaaagcagg gcatcgatcc
     8641 gctgccgttc tacaacccgc ccgccgaggt cgagaatcgc gagctcgaaa agcgctatcc
     8701 gctcgcgttc atctcgccgc cggcgcgcaa cttcctcaac tcgtcgttcg ccaacatcgc
     8761 gcggtttcgc gagctcgagc gcgagccgca cctcgacctg catccgaaag acgccgccga
     8821 gcgcggcatc gccgacggcg acatggtgcg agtgttcaac gaccgcggca gccagcggct
     8881 gcgcgcgcgc gtcaatggca agccgcgtcc cggcgtcgtg gtcgcgcctt cggtctggtg
     8941 gaagaagtac gcccccgacg gccgcaacgc caacaacctc acctcgcagc gcacgagcga
     9001 tctcggcggc ggcgccacgt tctacgactg ccgggtgcaa gtcgaaagag cgtgagcaag
     9061 gccgacgagc tgcggctgct gcgcgacgcg atcaagagtt ttcccgatgc cctggcggtg
     9121 tggagcgccg aggggcgcct gatcgcgtgc agcgacctct acgcgcggct tgccgcggca
     9181 acgccggcgg gcaatagcgc cgccgagtac cagagcgcgg acggccgctg gctgcggcgc
     9241 acgctgcggc gcgtcgaggg cggcggcagc gttgagctgg tcgccgacgt cagcgagctg
     9301 cgccgcgccg agcaggcgtc gcgcttcctc gggtaccacg acacgctcac cgggctgccc
     9361 aaccggcgcc tgctggacga ccggctgcgg caggcgattc acctcgccca gcggcgcgac
     9421 cggatggtcg cgaccctgct cgtgcgcctc gaggacttcc ggcaggtgaa cgacacgctc
     9481 ggcgagggcg caggcgatgc gctgttgcgc gaggtcgcgc agcgcctgag cgcctgcgtg
     9541 cgcaaggccg atacgctggc gcgcgccggc accgacgagt tcgcgctcgt ggtgtgcgac
     9601 ctcaaggacg gcgccgactg ccgcatcgtc gccgacaagc tgctgcaggc gctcgcgccg
     9661 gagctccgcg tcggcggccg gcgcctcaag ctcgcggtca gcgtgggcat ttcgctgttc
     9721 ccgtccgacg gcggcgacgg cgaggcgctg ctgcgcaacg ccgacgccgc ggtgctgcgt
     9781 gccaagcagc tcggccgcaa ccaggttcgt tttttcggcc gatgaagctc gcacgcttcc
     9841 tctggcccta ccggatgcgc attgccgtgg ccctgcttgc gctcctggtc acctcggcgt
     9901 gcgtgctggc gctcggccgc gggctgcagc acgtcgtcga ccgtggcttc ggcagcggcg
     9961 acccgcggct cctcgaccag gcgctcgcgg cgatgatcgc ggtggtcgtg gtgttcgcgg
    10021 cggcgacctg ggtgcgcttc tatttcatga tgagcaccgg cgagcgcgtg gtcaccgacc
    10081 tgcggcgcgc ggtgttcgat cacatcctgg gcctcgatcc ggcgttcttc gagaaggagc
    10141 gcaccggcga agtggtgtcg cggctcacca acgacgccac gctggtgcag caggtcatcg
    10201 gctacgggct gtcgatgttc gtccgcaacg gcctgatgat gatcggcgcc gcggcgatgc
    10261 tgttcgtcac gagctggaag ctcgcgctcc tggtgctggt cggcgcgccc gcgacgctgc
    10321 tgccgatcct gctgctcggc cgcagggtgc gagggctgtc gcgcgctagc caggaccgcg
    10381 tcgccgacgt gtccgcgtat gtcgacgagg caatccacga aattcgcacg gtgcaggctt
    10441 atgcgcacga ggacgcggat cgcgcgggct tcgcgcgcca cgccgaggcg gcgtacgcgg
    10501 ccggcgtcga gcgcatctcg tacaaggcat tcctgatcgc cgcggtgatc ctgatcgcct
    10561 tcagcgcggt cggcatcatc ctgtggatcg gcgggcacga cgtgctcgcc gggcggctca
    10621 cgcagggcga gctcgccgcc tttgtgttct atgccgcggt ggtcgcgatg ggcgcgggaa
    10681 cggtggcgga agtctggggc gagctgcagc gcgcggcggg cgcgaccgag cgtctggtcg
    10741 agctgctcga cacgccgccg cgcctcgtcg cgccggcggt gccggtcgcc gcgccgatac
    10801 ggtctccact aggagttagt tttgaagaag tgggttttgc gtacccggcg cgcgcagcga
    10861 cgcctgcgct cgtgcgcttc acgttacagg tcgcccccgg cgagcgcgtg gcgctggtcg
    10921 gaccttcggg ggcgggcaag tccacggtat tcgcgctgct cctgcgcttt tacgatccgc
    10981 agcagggcgc ggtgcggatc gacggcatcg acctgcggga gatggagccg ggcgcggccc
    11041 gccgcctggt cgccgtggtg ccgcaggaac cggtgatctt cgccgccagc gtgctggaaa
    11101 acgtccgcta cggccggccg gacgcgacgc gcgaggcggt cagcgcggcc tgcgagcaag
    11161 cctgcgcgct cgaattcatc gaacgcctgc cccacgggct cgacacgccg ctcggcgagc
    11221 gcggggtcac gctttccggc ggccagaagc agcgcctgtc gatcgcgcgc gcgctgctcg
    11281 ccgaccggcc gatcctgctg ctcgacgagg cgacgagctc gctcgacgcc gccgccgagc
    11341 gcctggtcca gcagggcctg gaagccctgg agaaaggacg caccacgctc gtcatcgcgc
    11401 accggctcgc cacggtgcag aatgccgggc gcattgtcgt tctcgagcgc ggcgaaattg
    11461 cggcgcaggg gtcgcacgcg gagctgatgc gccagggcgg gctctacgcg agcctggcgc
    11521 ggctgcagtt cctcggaggg agagatgaaa aggaccatcg cggtctgcgc gttattggtt
    11581 gaaagccgcc ggctcccagg atgaagtggg tggggatgct cgcggcggtg atcgcgtgcg
    11641 ccgctcaggc gcaaaccttc accgtcggtt caaagcgctt taccgagtcc tacatcctcg
    11701 gcgaaatcct cagccagacc gcgggcgcgg cgcacaagcc cgggctcggg aacaccgcga
    11761 tcctgctcga ggcgctcaag gccggcgcga tcgacctcta cccggaatac accggcacca
    11821 tcgcgcgcga gatcctcaag tccgaggagc ggctcgatct cgccgctctg aatcagaggc
    11881 tgaaatctct cggtctggcg gcgagcgtgc ccctgggctt cagcaacagc tacgcgctcg
    11941 gcatgcgccg caaggacgcc gagcgcctcg gcatcaagaa agcgtccgat ctggccgggc
    12001 atcctcagct gcgcttcggc ctgtcgcacg agttcctcgg ccgccgcgac ggctggccgg
    12061 gcctccagtc cgcctacggc ctgccgcaca agccgcgcgg cctcgatcac ggcatcgcct
    12121 acgaagcgct cgccgccggc gagatcgacg tcatggatct ctacaccacc gacgccaaga
    12181 tcgagcgcta tgcgatcgtg gcgctggccg acgaccgcgc gttctttccc gcctacgatg
    12241 cggtgctcct gtatcgcgca gacatcccgc agcgctttcc tgcagccttc gatgaactga
    12301 ggaagctcga gaagcgcatc gacgagcaga ccatggtgcg cctcaacgct cgcgccgagc
    12361 tcgacaaagt ggccttcgcc gatgtggcgc gggagtacct cggcgcgaag atcgagagca
    12421 cgaagggctt ctgggcggcg atgttcgcgc ccgatttcgg tcgcctgctg gccgagcacc
    12481 tgtggctcgt gttcgcttcg ctcgcgatcg cggtgctggt cggcgtgccg ctcgggctga
    12541 tcgccgcgaa gtacgggtgg gtcgcgcagc cggtgctgct attcaccggc gtgctgcaga
    12601 ccattccgtc gctcgcgctg ctcgccttcc tgattccggt caccggcacg atcggcgtct
    12661 ggccggctct gatcgcgctg tttctgtacg cccttctgcc gatcactcgc aacacgcatg
    12721 ccggcatcgc cggagtgccg cggggcttgg tgcaagcggg aaccgcgctg gggttgaggt
    12781 cccggcaaat tctgttgaaa gtcgaaatgc cgattgcaat gccggtgatc atggcgggcg
    12841 tgaagacttc cgcggtgatc agcgtcggca ccgcgacgat cgccgcattc atcggcgccg
    12901 gcgggttcgg cgagcgcatt tcgcaggggc tcgcgctcaa cgaccacgcc gtgctgctcg
    12961 ccggcgcgct gcccgcagcg gcgctcgcgc tggcggtgca cgccgcgttc gagatcgcgg
    13021 agcgctccct gtcgcggctg ccaaggtaga attccgcccc accgtttccc ggagaggtcc
    13081 ttgccgcagt tgatcggagt gccgcgcgag atcgaggcgg gcgagaagcg cgttgccacg
    13141 gttcccgacg tcgtcgagaa gctgatcaag ctggggttcc gggtcgcggt gcaatccggc
    13201 gccggcgacg ccgcgaactt cgccgacgac acctaccgcg cggcgggcgc cgagatcatc
    13261 ggcgatgccg agcgcctgtg gacgagcgcc gacatgatct tcaaggtgcg cgcgccgagc
    13321 atggcggaag tccagctgat gcgcgacggc actacctcat ctactacctt ggtgagcttc
    13381 atctggccgg cgcagaaccc gcagctgatg caggcgctgg cggcgaaaaa ggcgaccgtg
    13441 ctggcgatgg acagcgtgcc gcggatctcg cgcgcgcaga agctcgacgc gctctcgtcg
    13501 atggccaaca tcgccggcta ccgcgcggtg atcgaggcgg cgcaccactt cgggcggttc
    13561 ttcaccgggc agatcaccgc ggcgggcaag gtgcccccgg ccaaggtgtt catcatcggc
    13621 gccggtgtgg cgggcctcgc ggcgatcggc gcggcttccg gcctcggcgc gatcgtgcgc
    13681 gcgaacgaca cgcggcccga ggtcgccgac cagatcaagt cgctcggcgg cgagtacgtc
    13741 ggcgtcgact acgtcgaaga gggctccggg accggcggct atgccaaggt gatgagcgag
    13801 gggttccaga aggcccagcg tgaagtgttc gcgaggcagg cgaaggaagt cgacgtgatc
    13861 atcaccaccg ccctgatccc cggcaagccc gcaccccggc tgatcaccgc ggacatggtg
    13921 cggtcgatgc gccccggcag cgtgatcgtg gacctggcgg cggagcaggg cggcaactgc
    13981 gagctcaccg agcccggcca ggtgacggtg aagcacgggg tcacgatcat cggctacacc
    14041 gacctgccca gccggctcgc caagcagtcg agcacgctgt acgccaccaa cctgctgcgc
    14101 ctgaccgagg agctctgcaa gggtaaggac ggcgtcatca acgtgaacat ggacgacgag
    14161 gtgatccgtg gcaccac
//
LOCUS       DATBSZ010000308         8227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1361834, whole genome shotgun sequence.
ACCESSION   DATBSZ010000308 DATBSZ010000000
VERSION     DATBSZ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361834"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..699)
                     /locus_tag="VIG70_20100"
     CDS             complement(73..699)
                     /locus_tag="VIG70_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEY8252902.1"
                     /translation="MTRPLSLLETRVLGTLYEKQHTVPDSYPLTLNALVSGCNQKTSR
                     SPVMEATEAEVQAALDGLKSANFAMETSGGRVSRYSHNLERVLQVPSQSAAILTVLML
                     RGPQTAGELRINAERLHSFSDISAVQGFLDELAARAAGALVVELPRLPGARENRWAHL
                     LSGAPNIETIKKQEKQETSVEANVARLEAEVAALKDLVARIAKQVGVS"
     gene            complement(696..1898)
                     /locus_tag="VIG70_20105"
     CDS             complement(696..1898)
                     /locus_tag="VIG70_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEY8252903.1"
                     /translation="MPLEKPVSQLPGLRRILADFGASYAAAAFVAFLFATSGPVVIIL
                     SVGARGGLSESDIASWIFAAFCLNSVISVAFTLAYRQPLVFLWSIPGAVLVGPALGHL
                     TFPEVIGAFVATGVLMLLLGLSGWVRRAMDAVPMPVVMAMVAGVFLRFGVDLVLAFRD
                     ALWIALPMTAAFLVVSISRLERILPPLIAALITGAIAIGVLGAFKPPAGALFALAAPN
                     LYVPQFSWAAMVELVVPLAITVLAVQNAQGIAILAGNGHQAPVNAITAACGVGSIVTG
                     LFGAVSTCLTGPVNAVLSSSGEKPRQYTAALFFCLLAVAFGLLAPFFTRLLLATPPAF
                     IAALAGLAMLRVLQTAFTASFGGRFSLGALVCFLVTVADVPLFHIGAPFWGLIFGLGA
                     AWLLERSR"
     gene            1923..4022
                     /locus_tag="VIG70_20110"
     CDS             1923..4022
                     /locus_tag="VIG70_20110"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY8252904.1"
                     /translation="MAYLVGIDIGGTFTDCAIVDRAGKLLTTKVPSTPADFSRGMMDA
                     LDAGAQALGMKLDRFCRDIAFLSHGTTVGTNTIIQKKGAKVGLITTKGHEDAIHIMRG
                     SRGYGGRDIRKVVHFPETSKPQPIVPKRLIRGVSERVDCFGEVVVELNENEAEAAIRS
                     LVAEGVEAIAICFLWSFRNPDHELKVKKMVQRLAPSLFVTCSFDIAPKWGEYERVTAT
                     ALNAYLGPVMSGYLHKLNSSLKKLGYAHGLQITQCGGGTVPVERAGEAPLLTLDSGPV
                     SGVTASMFLGAAMGEKNVITTDMGGTSFDVSIIHDGRPAYSFVANTDQYEYFLPKVDL
                     QAIGAGGGSLVRVRPETRTMTVGPDSAGAFPGPVCYGRGGKMPTVTDAQVVLGYLDPD
                     NFAGGKMKLDKQSAADAIAAVGAQIGMKPVECAAGICRIVELQMADVIRKVTVEKGYD
                     PRDFVLFAFGGAGPAHAGVFARELGVRKIIVPQRKAASTWCAFGAAAADVLHIFEHSE
                     IMPTPVPARRINDALEALETKASALMKKESIAPGRQRFEFSLDVRHRGQINEVEVSLD
                     SPRLKDGYELPLRKRFVARYEKLYGRGSALADAQLELVVARLRARALTPRPRLVFTRK
                     NQGAIPASAKRHSRSIYWSDLGKFRQTPVFDGEKLATGNRIRGPAIVETADTTVVVHP
                     GRTLRLDALGNFEITFK"
     gene            4120..6393
                     /locus_tag="VIG70_20115"
     CDS             4120..6393
                     /locus_tag="VIG70_20115"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY8252905.1"
                     /translation="MNAPLKKLSDLAGVVFDGKRSGYVPPKQLRISPRLKLHKKAKGQ
                     IDPVTFEVVRHALWNVNEEHGATIQRLSGSPVAMYALDLNPSILTEDAEFVYFGPYMQ
                     YMSGVTDTQVKWTLENRAHNPGIADGDMFLANDPWVGAAHQMDVMLLCPVFWKGALFC
                     WVTNCLHQYDIGGITPGSFCPSARDAFDEGILIPPIKIIEKNEIRRDIEEVYLRSSRK
                     PSAVALDFRAQLAGNTTARDRILSLIKRYGAETVKGVMRKILDDGESAFLKKLARLPD
                     GVWRDRTYIECCRPGDRGVHRVALTLKKRGNKLVFENEGTDPQAGGINATYSGWRGAV
                     MVALNELMCWEQYYAVGGALRHVEFNPTPGTLNCADFPASVSTAPVQCMEISLYPAYN
                     VLSKMIYPDPELRRDTMCIGGTSQWPATIFRGIDQWGERYGYLLVDPIGGAIGAFSHA
                     DGISTGGQARTPICKLPNVEHTEQSFPVLFLYRKEICDSGGAGKWRGGLSAESCFIPH
                     GTDRITHDTLSSGNAIPTSTGMMGGYPGAVNRYRFLKDSDVLERMRRSELVGDIDDLK
                     GKPETLQLRQQDFPQNPSDVYAVLWSAAGGFGDPLERDPARVEDDVENGDVSEKTALE
                     VYGVVLGDARATGRRRADIRARRIQGTSKAPAKLGTPVRFLATANLAIHNNMRYGCAK
                     CSTDLGSIRENYKTHCVRNDLPIEASTPIAGDPRRFIDPLPQFRQFCCPGCGLAIENE
                     IAVADDPVLRDVEILSK"
     gene            6390..7439
                     /locus_tag="VIG70_20120"
     CDS             6390..7439
                     /locus_tag="VIG70_20120"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY8252906.1"
                     /translation="MKLSRRDALRDLVGLGLLALASSAANAQGDPELAGILGVDMHSH
                     AGGVHYRPLPQYHIARRMRQGRLAAACLCHTGDGPLLRRNSDGSITARQPAPYELYLH
                     TTERLSFFDALVRDQGFRRALKPGDLEAAHKEGVPAVVQAIEGCEFLDGRLERVGEVH
                     RRGVRHLQMVHFFKSELGDNQTEPPTQGGLTAFGREMIAECNRLGMVVDVAHATMKLV
                     ERAAKVCRTPLVLSHAPALREKAGPLSRVVSLQHAKLVAQTGGVVGIWTIVGTFKTLN
                     DWAEGVARAVDGLGIDHVGIGSDMGGAPENLVADYRDFPILLKRLRERGFSAEEIGKI
                     AGGNYVRVFNRSTHA"
     gene            7432..>8227
                     /locus_tag="VIG70_20125"
     CDS             7432..>8227
                     /locus_tag="VIG70_20125"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8252907.1"
                     /translation="MPDFSGVSYDEAMRRAREIVPLLRERAQKCEDARALLPENEKLL
                     HETGLFRYHQPKRFGGMELPFVAVVDITAELARGCPSTAWNVGNLGCHHWILGYYEPE
                     TQHELWDANPDVLIASSIALAAGRGRRSPGGFTVSGRWPFSSGVDNSDWNMLAVSVYE
                     DDKVVDWRLCLVPKSDYRVIDTWYAMGMVGTGSKDIEVKEVFVPERRALALQRCRGGL
                     EHPGAALNKGALFKVPIVASAGHPLSATCLGTAEGAFEHVLNTFKTR"
BASE COUNT         1490 a         2735 c         2755 g         1247 t
ORIGIN      
        1 cgcacaaaaa gaaggccctt ctgagccttt tctctcctcc cggcaatgcc gggcatcact
       61 ttccgcccag acctacgaaa cgccgacctg cttcgcgatg cgcgcgacca ggtccttcaa
      121 cgcggcaacc tccgcctcga gcctcgctac gttggcttcc accgaggttt cctgtttttc
      181 ctgttttttg atggtctcga tgttcggggc tccgctcagg aggtgggccc agcggttttc
      241 gcgcgcgccg gggagccgcg gcagctctac caccagggcg ccggcggcgc gcgcagcgag
      301 ctcgtcgagg aagccctgga ctgcggagat gtcggaaaag ctgtgcaggc gctcggcgtt
      361 gatgcgcaac tcccccgcag tctgcgggcc gcgcagcatc agcacggtga ggatcgcggc
      421 ggactgcgac ggcacctgca gcacacgctc gaggttgtgc gaatagcgcg acacccgccc
      481 cccgctcgtt tccatggcga agttcgcgct tttcaggccg tcgagcgccg cctggacctc
      541 ggcctcggtg gcctccatca ccggcgagcg gctggtcttc tggttgcagc cggagacgag
      601 cgcgttcagc gtcagcggat agctgtcggg aacggtgtgc tgcttctcat acaacgtgcc
      661 gaggacgcgg gtttcgagaa gcgaaagggg acgtgtcatc gtgaacgctc gaggagccac
      721 gcggcgccaa ggccgaatat taggccccag aacggcgcgc ctatgtgaaa gagcggaacg
      781 tcggcgaccg tcactaaaaa gcacaccagc gcaccaaggc tgaagcggcc gccgaaggat
      841 gccgtgaacg cggtctgcag gacccgcagc atcgcaaggc ccgcgagcgc ggcaatgaac
      901 gccggcggcg tcgccaggag cagccgcgta aagaacggcg cgagcagtcc gaaagcgacc
      961 gccagcaggc aaaagaagag cgcagccgtg tattggcggg gcttctcgcc gctgctcgac
     1021 agcaccgcgt tgaccgggcc ggtgaggcaa gtcgagaccg cgccgaagag cccggtgacg
     1081 atcgagccca ccccgcaagc ggcggtgatg gcgttgaccg gcgcctggtg cccgttgccg
     1141 gcgaggatcg cgatgccctg ggcgttctgc accgcgagca ccgtgatggc gagcggcacc
     1201 acgagctcca ccatcgccgc ccaggagaat tgcggcacat acaggttcgg cgccgccagc
     1261 gcgaacagcg cgccggcggg cggcttgaac gcgcctagaa cgccgatcgc gatcgccccg
     1321 gtgatcagcg ccgcaatcaa cggaggcaag atccgttcga gtctcgagat actgaccacc
     1381 agaaatgccg ctgtcatggg aagggcaatc catagcgcat cccggaaagc gagcaccagg
     1441 tcgacgccga agcgcaggaa cacgccggcg accatcgcca tcacgaccgg catcggcact
     1501 gcgtccatcg cgcgccgcac ccagcccgag aggccgagga ggagcatcag cacaccggtg
     1561 gcgacgaagg cgccgatcac ctccggaaac gtgagatgcc cgagcgccgg cccgaccagc
     1621 accgcgccgg ggatgctcca caggaagacg agcggctggc ggtaggcgag cgtgaacgcg
     1681 accgagatca cgctgttcag gcagaacgcg gcgaagatcc aggaggcgat gtcggattcg
     1741 gacagcccgc cgcgcgcgcc gaccgagaga atgatcacca ccgggccgct cgtcgcgaac
     1801 aggaaagcga cgaacgcagc cgcggcatac gacgcgccga agtcggcgag gatgcgccgg
     1861 agcccgggca gttgcgagac aggtttctcc agcggcatgc ttcggtagct aatatagcgt
     1921 taatggctta tctggtcggc atcgacatcg gcggtacctt caccgactgc gcgatcgtcg
     1981 accgcgccgg caagctgctc accaccaagg tgccgtcgac gccggcggat ttctcgcgcg
     2041 gcatgatgga cgcgctcgac gcgggcgcgc aggcgctcgg catgaagctc gaccggttct
     2101 gccgcgacat cgcgttcctg tcgcacggca ccaccgtcgg caccaacacc atcatccaga
     2161 agaaaggcgc caaggtcggc ctcatcacca ccaaggggca cgaggacgcg atccacatca
     2221 tgcgcggctc gcgcggctac ggcgggcgcg acatccgcaa ggtggtgcat ttccccgaga
     2281 cctcgaagcc gcagccgatc gtgcccaagc gcctgatccg cggcgtgtcg gagcgcgtgg
     2341 attgcttcgg cgaagtcgtg gtcgaattga acgagaacga agccgaggct gcgatccgca
     2401 gcctggtcgc ggaaggcgtc gaggcgatcg cgatctgctt cctgtggtcc ttcaggaatc
     2461 ccgatcacga gctgaaggtg aagaaaatgg tgcagcggct tgcgccgtcg ctcttcgtca
     2521 cctgctcctt cgacatcgcg cccaagtggg gcgagtacga gcgcgtcacc gccacagcgc
     2581 tgaacgcgta tctaggtccg gtgatgtcag gctatttgca caaactgaat tcttctttga
     2641 agaagcttgg ctatgctcac ggcctgcaga tcacgcaatg cggcggcggc accgtgcccg
     2701 tcgagcgcgc gggcgaggcg ccgctgctga cgctcgactc ggggccggtg tccggcgtga
     2761 cggcgtcgat gttcctcggc gccgcgatgg gcgagaagaa cgtcatcacc accgacatgg
     2821 gcggcacctc gttcgacgtc tccatcatcc acgacggaag accggcgtac tccttcgtcg
     2881 cgaacaccga ccagtacgag tacttcctgc ccaaggtcga cctgcaggcg atcggcgccg
     2941 gcggcggcag cctggtgcgg gtgcggccgg agacgcgcac catgacggtg ggcccggaca
     3001 gcgccggggc gtttcccgga ccggtgtgct acgggcgcgg cggcaagatg ccgacggtga
     3061 ccgatgcgca ggtcgtgctc ggctatctcg atccggacaa cttcgccggc ggcaagatga
     3121 agttggacaa gcaatccgcg gccgacgcga tcgcggcagt cggtgcacaa atcggaatga
     3181 agcccgtcga atgcgccgcg ggcatctgcc gcatcgtcga gctgcagatg gccgacgtga
     3241 tccgcaaggt gacggtggag aagggctacg acccgcgcga cttcgtgctg ttcgccttcg
     3301 gcggcgcggg cccggcgcac gccggcgtgt tcgcgcgcga gctcggggtc cggaaaataa
     3361 tcgttcctca gcgaaaggcg gcttctacct ggtgcgcgtt cggcgccgcc gcggccgacg
     3421 tgctgcacat cttcgagcac agcgagatca tgccgacgcc ggtgccggcg cggcggatca
     3481 acgacgcgct cgaggcgctc gagacgaagg cctccgcgct gatgaagaag gagagcatcg
     3541 cgccggggcg ccagcgcttc gagttctcgc tcgacgtgcg ccaccgcggc cagatcaacg
     3601 aggtcgaagt gtccctggat tctccgcgcc tgaaggacgg ctacgagttg ccgctgcgca
     3661 agcgcttcgt cgcgcgctac gagaagctct acgggcgcgg ctcggcgctc gccgacgcgc
     3721 aactcgagct ggtcgtcgcc cggctgcgcg cgcgggctct gacgccgcgg ccgagattgg
     3781 ttttcacaag gaaaaatcaa ggggcaatac cggcgagcgc aaagcgccat tcccgctcga
     3841 tctactggtc cgatctgggc aagttccgcc agactccggt gttcgacggc gagaagctcg
     3901 caacgggcaa ccggatccgg gggcccgcga tcgtcgagac cgccgacacc acggtcgtgg
     3961 tccatcccgg ccgaacgctg cggctcgacg cgctcggcaa cttcgagatc accttcaagt
     4021 gacgacgcac gacaagaaca gcaaaaacag ggacagacca cgttttattt attcctgcgg
     4081 gggtcgaaaa acgtggtctg tccctgattt ggtggttgca tgaatgcacc gctgaagaag
     4141 ctcagcgacc tcgccggcgt ggtgttcgac ggcaagcgca gcggctacgt gccgccgaag
     4201 cagctcagga tttctcccag gctgaaatta cataagaaag ccaagggcca gatcgatcct
     4261 gtgaccttcg aggtggtgcg ccacgcgctg tggaacgtga acgaggagca cggcgcgacc
     4321 atccagcgcc tgtcgggctc gccggtggcg atgtacgcgc tcgacttgaa cccgtcgatc
     4381 ctcaccgagg acgccgagtt cgtctatttc gggccgtaca tgcagtacat gtccggcgtc
     4441 accgacacgc aggtgaagtg gacgctcgag aaccgggccc acaatcccgg catcgccgac
     4501 ggcgacatgt tcctcgccaa cgacccctgg gtgggcgcgg cgcaccagat ggacgtgatg
     4561 ctgctgtgcc cggtgttctg gaagggagcc ctcttctgct gggtgacgaa ctgcctgcac
     4621 cagtacgaca tcggcggcat caccccggga agcttctgcc cctcggcgcg cgatgcgttc
     4681 gacgagggaa tcctgatccc gcccatcaag atcatcgaaa agaatgaaat ccggcgcgac
     4741 atcgaggagg tgtacctcag gagcagccgg aagccgagcg cagtggccct cgatttcagg
     4801 gcgcaactgg cggggaatac cacggcccgg gaccggatcc tgtctctcat aaagagatat
     4861 ggcgcggaga ccgtgaaggg cgtgatgcgc aagatcctcg acgacggcga gagcgcgttc
     4921 ctgaagaagc tcgcgcgcct gcccgacggg gtctggcggg accggaccta catcgagtgc
     4981 tgccggccgg gcgaccgcgg cgtccacagg gtcgcactga ccctgaaaaa gcgcggcaac
     5041 aagctggtgt tcgagaacga gggaacggac ccgcaggccg gcggcatcaa cgcgacctac
     5101 tccggctggc gcggcgcggt catggtcgcg ctgaacgagc tcatgtgctg ggagcagtac
     5161 tacgcggtcg gcggcgcgct gcgccacgtc gagttcaacc cgacccccgg gacgctcaac
     5221 tgcgcggatt tccccgcctc ggtgtcgacc gcgccggtgc agtgcatgga gatctcgctc
     5281 tatcccgcct acaacgtgct ctcgaagatg atctatcccg acccggagct gcgccgcgac
     5341 acgatgtgca tcggcggcac cagccagtgg ccggcgacga tcttccgcgg catcgaccag
     5401 tggggcgagc gctacggcta cctgctggtc gatccgatcg gcggcgcgat cggcgcgttc
     5461 tcgcatgccg acggcatctc caccggcggc caggcgcgca cgccgatctg caagctgccg
     5521 aacgtcgagc atacggagca aagcttcccg gtcctgtttc tgtaccggaa agagatctgt
     5581 gattccggcg gcgcgggcaa atggcgcggc ggcctgtcgg ccgaatcgtg cttcatcccg
     5641 cacggcaccg accgcatcac gcacgacacg ctgtcctcgg gcaacgcgat cccgacctcg
     5701 accggcatga tgggcggcta ccccggcgcg gtgaaccgct accggtttct caaggacagc
     5761 gacgtgctcg agcgcatgcg gcgatccgaa ctggtcggcg acatcgacga cctgaaaggc
     5821 aagccggaga ccttgcagct tcgccagcag gattttcccc agaatccaag tgacgtgtac
     5881 gcggtgctct ggtcggcggc cgggggcttc ggcgacccgc tcgagcgcga cccggcgaga
     5941 gtcgaagacg acgtggagaa cggcgacgtc tccgagaaga ccgcgctcga ggtctacggc
     6001 gtggtgctcg gcgacgccag ggcgaccggg cggcgccgcg cggacatccg cgcgcggcgg
     6061 atccagggaa cctcgaaagc accggccaag ctgggcacgc cggtgcgctt cctcgccacc
     6121 gcgaacctcg ccatccacaa caacatgcgc tacggctgcg ccaagtgcag cacggacctg
     6181 ggctcgattc gggaaaatta caagacccac tgcgtgcgca acgaccttcc gatcgaggcc
     6241 tcgaccccga tcgcgggaga cccgcggcgc ttcatcgacc cgctgccgca gttccgccag
     6301 ttctgctgcc cgggctgcgg cctcgcgatc gagaacgaga tcgccgtggc ggacgatccg
     6361 gtgctgaggg acgtggaaat cctgtcgaag tgaaactctc ccgccgcgac gcgctgcgcg
     6421 acctggtcgg gctggggctg ctggccctcg ccagcagtgc ggcgaatgcc caaggcgacc
     6481 cggagctcgc gggcattctc ggcgtggaca tgcactccca cgcgggcggc gttcactacc
     6541 gtcccctgcc gcagtaccac atcgccaggc gaatgcggca aggccggctc gccgccgcct
     6601 gcctttgcca cacgggcgac ggtccgctcc ttcgccgcaa ctccgacggg agcatcaccg
     6661 cccgccagcc cgcgccatac gagctctacc tccacaccac ggagcgtctg agctttttcg
     6721 atgcgctggt tcgcgaccag ggattccggc gcgcgctgaa acccggcgac ctggaggccg
     6781 cgcacaagga gggcgtgccc gccgtcgtgc aggccatcga aggctgcgag ttcctcgacg
     6841 gcaggctgga gcgcgtcggc gaggtgcacc ggcgcggcgt gcgccatttg cagatggtcc
     6901 atttcttcaa gagcgagctc ggcgacaacc agaccgagcc ccccactcag ggcggcctca
     6961 ccgccttcgg cagggagatg atcgcggagt gcaaccggct gggcatggtg gtggacgttg
     7021 cccacgccac gatgaagctc gtggagcggg ccgccaaggt gtgcaggacg ccgctcgtcc
     7081 tctcgcacgc ccctgcgctc cgcgaaaagg ccgggccgtt gtcccgcgtc gtctcgctcc
     7141 agcatgcgaa gctcgtggcg cagaccggcg gcgtggtcgg aatctggacc atcgtcggca
     7201 cgttcaagac cctcaacgac tgggcagagg gcgtggcccg ggccgtggac ggcctgggga
     7261 tagaccacgt cgggatcggc tcggacatgg ggggcgcgcc ggagaatctc gtcgcggact
     7321 accgggactt cccgatcctg ctgaagcggc tgcgggagcg cggcttttcc gccgaggaga
     7381 tcgggaagat cgccggcggc aactacgtgc gggtgttcaa ccggtccacc catgcctgac
     7441 ttctccggcg tctcgtacga cgaggcgatg cgccgcgcgc gcgagatcgt cccgctgctg
     7501 cgcgagcggg cgcagaagtg cgaggacgcg cgcgcccttc tgcccgagaa cgagaagctg
     7561 ctgcacgaga ccgggctctt ccgctaccac cagccgaagc gcttcggcgg catggagctg
     7621 ccttttgtcg cggtggtcga catcaccgcc gaactcgcgc gcggctgccc gtcgaccgcg
     7681 tggaacgtcg gcaacctcgg ctgccaccac tggatcctcg gctactacga gcccgagacg
     7741 cagcacgagc tgtgggacgc gaatcccgat gtcctcatcg cctcttcgat tgctcttgcg
     7801 gcagggcgtg gccggcggtc ccccggggga ttcactgtca gcggccgctg gcccttctcc
     7861 tcgggcgtcg ataattccga ctggaacatg ctcgcggtca gcgtctacga agatgacaag
     7921 gtcgtcgact ggcgcctgtg cctcgtaccg aagagcgatt acagggtgat cgacacctgg
     7981 tacgcgatgg gaatggtcgg caccgggagc aaggacatcg aggtcaagga ggtgttcgtc
     8041 cccgagcgac gcgcactcgc cctgcagcgc tgccgcggcg gtttggagca tcccggcgct
     8101 gcgctgaaca agggcgcgct gttcaaggtt ccaatcgtcg cgtcggccgg ccatccgctc
     8161 tccgcgacct gcctcggaac cgccgaaggc gccttcgagc acgtcctgaa tacattcaag
     8221 acccggg
//
LOCUS       DATBSZ010000309         4055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1362841, whole genome shotgun sequence.
ACCESSION   DATBSZ010000309 DATBSZ010000000
VERSION     DATBSZ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362841"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..483
                     /locus_tag="VIG70_20130"
     CDS             223..483
                     /locus_tag="VIG70_20130"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8252908.1"
                     /translation="MLHLRDLHCFTRVYESRSFSRAADSLDTVQSLVSTRIQRLEQFV
                     GAPLFVRLHRGVVSTEKGELLYQHAKRVLRDVAELESVFREP"
     gene            complement(480..1655)
                     /gene="ccmI"
                     /locus_tag="VIG70_20135"
     CDS             complement(480..1655)
                     /gene="ccmI"
                     /locus_tag="VIG70_20135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEY8252909.1"
                     /translation="MTLFWLAGAALAAVALGLVLRPLLFRGSKSSVSRREANLSIYRD
                     QLRELDADLAAGTLAQADHERARREIEARLLEDVAVAPEAAPARGGRALVAAIAVAIP
                     LAALGVYLLVGNPRALSLQFEHQVNAQQLEAMVERLAARLRESPDDIEGWKLLGRSYG
                     ALGRFPEAVDAYVKAATRAPRDAQILADLAEVLAMSRGQRLEGEPEKLVLRALELDPQ
                     NIKALALAGSAAFARKDFAGAVRYWQRILPLVAADSEDARSIQANIAEAQSFLKPGAA
                     SVAALKGSVRLAPELKAKASPDDTVFIFARAAQGPPMPLAVKRAKVRELPLEFTLDDS
                     MAMAPGMQLSGFPRVIVGARVSKSGSATPQPGDLQGASATVANDASSVTVVIDSVVR"
     gene            complement(1652..2074)
                     /locus_tag="VIG70_20140"
     CDS             complement(1652..2074)
                     /locus_tag="VIG70_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEY8252910.1"
                     /translation="MAAARVLLLWLACAAALAAENPGLEKRTAALAHELRCLVCQNQT
                     LADSNAPLAVDLRNQIREQLAAGKSEQDVLDFMVARYGEFVLYRPPLRAATLALWVGP
                     FVFLALGVWLLARRLARRRAAEPELSGAERERAAKLLE"
     gene            complement(2061..2582)
                     /locus_tag="VIG70_20145"
     CDS             complement(2061..2582)
                     /locus_tag="VIG70_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEY8252911.1"
                     /translation="MKKIWLPLGFIALIGLLAAGLRLNPREVPSPLIGKPAPHFELPL
                     LHQPDKRFTQKDMLGKVWVLNVWASWCPPCLVEHPVVTELARTVPVIGLNYKDAREDA
                     LPWLKRNGDPYQFSVYDAAGRIAIDYGVYGVPETYVMDRKGVIRYKHIGPLTREVAQT
                     RLRTLVNELNGGG"
     gene            complement(2579..>4055)
                     /locus_tag="VIG70_20150"
     CDS             complement(2579..>4055)
                     /locus_tag="VIG70_20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY8252912.1"
                     /translation="FPAPPDGRDLNPLLQDPGMVLHPPMLYMGYVGLSVAFSFAVAAL
                     LSGRLDAAWARWSRPWTTVAWCFLTVGIALGSGWAYYELGWGGWWFWDPVENASFMPW
                     LVATALIHSLAVTEKRGGFRSWTVLLAIVAFGLSLIGTFLVRSGVLTSVHAFAVDPKR
                     GIFILMLLALFIGGALALYAWRTSRIGLGGAFEPVSRESLLLGNNVLLVAAAGTVFLG
                     TLYPLLIDAVGAGKISVGPPYFETVFVPLMAPALFLMGVGPLARWKRASLPELAVRLR
                     WALGVSLALGFLSPLLFGNWSAMVGFGLALAIWIVSTALLTLKKSLRQTRAHYGMVVA
                     HIGVAAFVVGVTLVKGYESEQDAKMKPGDAVELAGYAFRLEQISNVRGPNYAAAQAKV
                     VVSRNGKPVKVMYPERRHYTVQDQVMTEAAIDPGLTRDLYVSLGDPLEGGAWLIKVQH
                     KPFIDWIWGGCLIMALGGLLAASDRRYRFAIRERRAAEAAA"
BASE COUNT          690 a         1405 c         1382 g          578 t
ORIGIN      
        1 atggggtgct gctcttcttt tttcttttat gacgcgacat caaagcacaa ggggaaaccc
       61 cggctcaatc accgaaaagt gatatctcgg atccgggtgt caacaaagcc tttgcatagg
      121 ctgcttgggg cgccgtgtcc gattccattg acaaatctgg tgaagtggaa tgtgatggca
      181 attagaaaaa caagaacgat cttgagagca gggaggtgcc atatgctcca tctcagggat
      241 ttgcactgct tcacgcgggt gtatgaatcg cgcagcttca gccgggcggc ggacagcctg
      301 gacacggtcc aatccctcgt ttcgacgcgg atacagaggc tcgagcagtt cgtcggcgcg
      361 cccctgttcg tgcggctgca tcgcggggtg gtgtcgaccg aaaaaggcga gctgctgtat
      421 cagcacgcga agcgcgtgct gcgcgacgta gccgagctgg aatcggtgtt ccgagaacct
      481 taacgcacca cgctgtcgat cacgaccgtg acgctgctgg cgtcgttggc cacggtcgcg
      541 ctcgcgccct gcaggtcgcc cggctgcggc gtggcgctgc ccgactttga cacccgcgcg
      601 ccgacgatga cgcgcggaaa gccggacagc tgcatgccgg gcgccatggc catcgaatcg
      661 tccagcgtga actcgagcgg cagctcgcgc accttcgcgc gcttcaccgc gagcggcatc
      721 ggcgggccct gcgcggcgcg cgcgaaaatg aacacggtgt cgtccggcga cgccttggcc
      781 ttgagctccg gcgcaagccg cacgctgccc ttcagcgccg ccaccgacgc ggcgccgggc
      841 ttcaggaacg actgcgcctc ggcgatgttc gcctggatcg agcgcgcgtc ctcggaatcg
      901 gcggcgacga gcggcaggat gcgctgccag tagcgcactg caccggcaaa gtccttgcgc
      961 gcgaacgccg cgctgccggc gagcgcgagc gccttgatgt tctgcgggtc gagctcgagc
     1021 gcgcgcagca cgagcttctc gggctcgccc tccagtcgct gcccgcggct catcgccagc
     1081 acttcagcga ggtcggccag gatctgcgcg tcgcgcggcg cgcgcgtcgc cgccttgacg
     1141 taggcatcga ccgcctcggg aaagcgcccg agcgcgccgt acgagcgccc gaggagcttc
     1201 cagccctcga tgtcgtcggg gctctcgcgc agccgcgccg cgagccgctc gaccatggct
     1261 tcgagctgct gggcattcac ttggtgctcg aactggagcg agagcgcgcg gggattgccg
     1321 acgagcagat acacgcccag cgctgccagg ggaattgcca cggcaatagc cgcgaccaac
     1381 gcgcggccgc cgcgcgcggg cgccgcctcg ggcgccaccg ccacgtcctc gagcagccgg
     1441 gcctcgatct cgcgccttgc gcgctcgtgg tcagcctgcg ccagggtgcc ggcggcaagg
     1501 tcggcgtcga gctcgcgcag ctggtcgcgg tagatcgaga ggttggcctc gcggcgcgag
     1561 accgatgatt tcgacccgcg gaacagcaat ggccggagca cgaggccgag cgcgaccgcc
     1621 gcgagcgcgg cgccggccag ccagaacagc gtcattcgag cagcttcgcc gcgcgctcgc
     1681 gctccgcgcc ggagagctcg ggctcggcgg cgcgccgcct ggccagccgg cgcgcgagca
     1741 gccagacgcc gagcgcgagg aacacgaacg ggccgaccca gagcgcgagc gtcgccgccc
     1801 tgagcggcgg acggtacagc acgaactcgc cgtagcgcgc caccatgaag tcgagcacgt
     1861 cctgctcgct cttgcccgcg gcgagctgct cgcggatctg gttgcgcagg tcgacggcga
     1921 gcggcgcgtt ggagtcggcg agggtctggt tctggcaaac caggcagcgc agctcgtgcg
     1981 caagcgcggc ggtgcgcttt tcgagcccgg ggttctcggc cgccagcgcc gccgcgcagg
     2041 ccagccacag caggagcacc ctagccgccg ccattcagct cgttcaccag cgtgcgcagc
     2101 ctggtctggg cgacttcgcg cgtgagcggt ccgatgtgct tgtagcggat cacgcccttc
     2161 cgatccatca cgtacgtttc aggtacgccg tacaccccgt agtcgatcgc gatgcgcccg
     2221 gcggcgtcgt acaccgagaa ctggtaagga tcgccgttgc gcttgagcca gggcagcgcg
     2281 tcctcgcgcg cgtccttgta gttcaggccg atcaccggaa cggtcctggc gagctcggtg
     2341 accaccgggt gctcgaccag gcacggcggg caccaggagg cccacacgtt cagcacccag
     2401 accttgccga gcatgtcctt ctgcgtgaag cgcttgtcgg gctgatgcaa gagcggcagc
     2461 tcgaaatgcg gcgccggctt gccgatgagc ggcgaaggca cctcgcgcgg attgagccgc
     2521 agcccggcgg cgagcaggcc gatcagggcg atgaagccga gcggcagcca gatcttcttc
     2581 acgccgccgc ctcggccgcg cgcctttccc ggatcgcgaa acggtatctg cggtcgctcg
     2641 ccgcgagcaa gccgccgagc gccatgatca ggcagccgcc ccagatccag tcgatgaagg
     2701 gcttgtgctg caccttgatc agccaggcgc cgccctcgag cgggtcgccg agcgagacgt
     2761 agaggtcgcg cgtcaggccc gggtcgatcg ccgcctcggt catcacctgg tcctgcacgg
     2821 tgtagtggcg ccgctccggg tacatcacct tcaccggctt gccgttgcgc gacaccacga
     2881 ccttcgcctg cgcggcggcg tagttcggcc ccctcacgtt cgatatctgc tccagccgga
     2941 aagcgtagcc ggccagctcc accgcgtcgc cgggcttcat cttcgcgtcc tgctcgctct
     3001 cgtagccctt caccagcgtt acgccgacca cgaaggcggc aaccccgata tgcgcgacca
     3061 ccatgccgta gtgagctcgg gtttgtcgaa gactcttctt caaagtcaga agcgcggtgc
     3121 tgacgatcca gatcgcgagc gcgaggccga agccgaccat ggcgctccag ttgccgaaaa
     3181 gcagcgggga gaggaaaccg agcgccaggc tcacgcccag cgcccagcgc aaccgcaccg
     3241 cgagctccgg caggctggcg cgcttccagc gcgccagcgg gcccacgccc atcaggaaga
     3301 gcgccggcgc catcagcggc acgaacacgg tctcgaaata cggcggaccg accgagatct
     3361 tgccggcgcc gactgcgtcg atcagcagcg gatagagcgt gccgaggaac acggtgccgg
     3421 ccgcggcgac caggaggacg ttgttcccca gcagcaggct ctccctggac acgggctcga
     3481 acgcaccccc caggccgatg cgcgaagtgc gccacgcgta aagcgcgagc gcgccgccga
     3541 tgaacagcgc cagcagcatc aggatgaaga tgccgcgctt cgggtcgacc gcgaacgcat
     3601 gcaccgaggt cagcacgccg gagcgcacga ggaaggtgcc gatcaggctc aggccgaacg
     3661 cgacgatggc gagcagtacg gtccagctgc ggaacccgcc gcgtttctcg gtcacggcaa
     3721 gcgaatggat gagcgcggtg gcgacgagcc agggcatgaa ggaggcgttc tccaccgggt
     3781 cccagaacca ccagccgccc cagccgagct cgtagtacgc ccagccgctg ccgagcgcga
     3841 tgccgacggt gaggaaacac caggcgaccg tggtccaggg ccgtgaccag cgcgcccagg
     3901 cggcgtcgag ccggccggag agcagcgccg cgaccgcgaa cgagaacgcc accgacagtc
     3961 cgacgtaccc catgtagagc atcggcgggt gcagcaccat ccccgggtcc tgcagcagcg
     4021 gattcaggtc gcgcccgtcc ggcggcgcgg ggaac
//
LOCUS       DATBSZ010000310         2903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2046967, whole genome shotgun sequence.
ACCESSION   DATBSZ010000310 DATBSZ010000000
VERSION     DATBSZ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046967"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VIG70_20155"
     CDS             <1..624
                     /locus_tag="VIG70_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8252913.1"
                     /translation="FRFRGASAKVNLALGELPDFTCLPADGINGRRALRGAISISPSV
                     AYLERAYDEAKYGEVSRRPYMDIVIPSMLDPSMAPPGKHVMSIFVQYAPYHVNGGWTD
                     ERREALGNTVVETLSQYAPNLKSAILHRQVITPADIERIVGLAEGNIFQGELSLQQMF
                     FLRPVPAWAKYRTPLAGLYQCGAGTHPGGGVMGASGRNAALQILGVA"
     gene            621..1979
                     /locus_tag="VIG70_20160"
     CDS             621..1979
                     /locus_tag="VIG70_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8252914.1"
                     /translation="MSSVIVIGGGADALVAAQILARAGREVITIEGAAAPPDDGWVPR
                     KVLRELRLEGLELEWPDPWLTVPLPGGGRLELWRDMARTVEAIRQFSPRDAERWPEFC
                     DRMARLARFLETVYSAPPPDPLSAGFALRARRLGRRGLADLLRILPMPAAELLDDWFE
                     SDVLKGALGAAGILHLLQGPRSGGTAFRLLHHHLGCPPGVFRPPRSNVMDLLHKLPRV
                     ELQPAEVLRITVRGGAVAGVVLKDGRELAASTVVSSADPRRSLLELVEPGWLDPELVR
                     TLRHMRRRGVAARLRLQLDRSPGFATLALAPSLDYLERAYDDAKHGRVSQRPYVEARC
                     DERQRMEVLVQYVPYGVDCSNLKDAVLALLSEHWGGAQPQHAELLAPRELEEFEGWPE
                     GQPHHAELSLDQALWMRPVPELARYRTPIQGLWLCGPAMHPGAGIAGAAGMHCAHAIL
                     GG"
     gene            1960..>2903
                     /locus_tag="VIG70_20165"
     CDS             1960..>2903
                     /locus_tag="VIG70_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252915.1"
                     /translation="MRSWAVELCLVFWAGSAIGAERLPGYGADRSTFTVSGVSSGGYM
                     AVQVHVAHSSRVTGIGAVAAGPYYCAQGSLWTALNHCMSPGAWSPLPRPEALKGHADR
                     FVRDKLVDPASNLASARVWLFAGMRDRTVEPEVVSALEAFYRLFNAKPVLVKDKPAGH
                     GMVTENAGNACGATEPPFINDCDYDAAGELLRHLLGSLSPPAAKAGGRLLSFDQREFA
                     DVYKISMADSGYAYVPLACDSQACKVHVAFHGCRQGAETIGERFVREAGFNRWADTNR
                     LIVLYPQAIARYWWVYNPRGCWDWWGYTGSRYATKEGA"
BASE COUNT          407 a          999 c         1038 g          459 t
ORIGIN      
        1 tttcgcttcc gcggcgcgtc ggccaaggtg aacctggcgc tcggcgagct gcccgatttc
       61 acctgcctgc ccgccgatgg gataaacggc aggcgcgccc tgcgcggcgc gatctcgatc
      121 agcccgagcg tcgcgtacct cgagcgcgcg tacgacgagg cgaaatacgg cgaagtctcg
      181 cgccggccgt acatggatat cgtgattccc tcgatgctcg atccctcgat ggcgccgccg
      241 ggaaagcacg tgatgtcgat cttcgtccag tacgcgccct accacgtgaa cggaggctgg
      301 accgacgagc gacgcgaggc gctgggaaac acggtggtcg agacgctctc gcagtacgcg
      361 ccgaacctga agtcggcgat cctgcaccgg caggtgatca cgcccgccga catcgagcgc
      421 atcgtcggcc tcgccgaggg caacattttc cagggcgagc tttcgctgca gcagatgttc
      481 ttcctgcggc cggtgccggc ttgggcgaag taccgcacgc cgctcgccgg cctctaccag
      541 tgcggggcgg gaacgcaccc gggcggcggc gtgatgggcg cgtcgggccg caacgcggcg
      601 ctccagattc tcggggtggc atgagcagcg tgatcgtcat cggcggcggc gccgacgcgc
      661 tggtggcggc gcagatcctc gcgcgcgccg gccgcgaggt gatcacgatc gaaggcgcag
      721 ccgcgccgcc cgacgatggc tgggtgccga ggaaagtgct acgcgagctg cgcctcgagg
      781 gcctcgagct cgagtggccg gatccgtggc tcacggttcc gctgcccggc ggcgggcgtc
      841 tcgagctgtg gcgggacatg gcgcggaccg tggaggcgat ccggcagttc agcccgcgcg
      901 acgccgagcg ctggccggaa ttctgcgatc gcatggcgcg gctcgcgcgc ttcctggaaa
      961 ccgtctattc agcgccgccg cccgatccgc tgagcgcggg cttcgcgctg cgcgcgaggc
     1021 gcctgggcag gcgcgggctc gccgacctcc tgcgcatcct gccgatgccc gcggcggagc
     1081 tgctcgacga ctggttcgaa agcgacgtgc tgaagggcgc gcttggcgca gccgggatcc
     1141 tgcacctcct gcaggggccg cgctccggcg gcaccgcgtt ccggctgctg caccatcacc
     1201 tgggctgtcc gcccggggtc ttccgcccgc cgcgctcgaa cgtcatggac ctgctgcaca
     1261 agctgccccg cgtcgagctg cagccggccg aggtgctgcg gatcacggtg cgcggcggtg
     1321 cggtcgccgg cgtggtcctg aaagacggcc gcgagctcgc ggcgtccacg gtcgtgagca
     1381 gcgcggatcc gcgccgcagc ctgctcgagc tggtggagcc cggctggctc gatcccgagc
     1441 tcgtgcgcac gctgcgccac atgcgccggc gcggcgtcgc agcgcgcctg cggctccagc
     1501 tcgaccgctc tcctggcttc gcgacgctcg cgcttgcgcc ctcgctcgac tacctggagc
     1561 gggcctacga cgacgcaaag cacggccgcg tgtcgcagcg gccttacgtc gaagcgcgat
     1621 gcgacgagcg gcagcgcatg gaagtcctcg tgcagtacgt gccctacggg gtcgattgct
     1681 cgaatctgaa ggatgccgtg ctggcgctgc tctcggagca ctggggcggg gcgcagccgc
     1741 agcacgcgga gcttcttgcg ccgcgcgagc tggaggagtt cgagggctgg ccggaagggc
     1801 agccgcacca cgccgagctg agcctcgatc aggccctgtg gatgcgaccc gtgccggagc
     1861 tggcgcgcta tcgcaccccg atccaaggcc tctggctttg cggccctgcc atgcacccgg
     1921 gcgcgggcat cgccggcgcc gcgggaatgc actgcgcgca tgcgatcctg ggcggttgag
     1981 ctctgccttg tcttctgggc cggctcggcg atcggggcgg aacggctgcc cggctacggc
     2041 gccgatcgct cgacgtttac ggtgtccggg gtctcgtccg gcggttacat ggcggtgcag
     2101 gtccatgtcg cgcattcgag ccgcgtaacc ggtatcgggg ccgttgccgc gggaccctac
     2161 tattgcgcgc agggcagctt gtggaccgcg ctcaatcact gcatgtctcc gggcgcctgg
     2221 agcccgctgc cgcggcccga ggctctgaaa ggccatgccg atcgcttcgt gcgcgacaag
     2281 ctcgtcgacc cggcgagcaa cctcgcgagc gcgcgcgtgt ggctgttcgc cgggatgcgc
     2341 gatcgcaccg tggagcccga ggtcgtgtcc gcgctggaag ctttttaccg cctcttcaat
     2401 gcgaaaccgg tcctcgtcaa agacaagccg gccggccacg ggatggtcac cgagaacgct
     2461 ggcaacgcgt gcggggcgac cgagccgccc ttcatcaacg attgcgacta cgacgccgcg
     2521 ggagagctgc tgcgtcatct tctcggcagc ctttcgccgc cggctgccaa ggcaggtggg
     2581 cgcctgctct ccttcgacca acgagagttt gcagatgtgt acaagatcag catggcggac
     2641 agcggttacg cctacgtccc gctcgcctgc gattcccagg cctgcaaggt gcacgtcgcg
     2701 tttcacggct gccgccaagg cgccgagacg atcggggaac gtttcgtcag ggaggccggc
     2761 ttcaaccgct gggcggacac gaaccggctg atcgtgctct acccgcaggc gatcgcgcgc
     2821 tactggtggg tctacaaccc ccgggggtgc tgggactggt ggggctatac cggctcgcgc
     2881 tatgccacca aggagggcgc gca
//
LOCUS       DATBSZ010000311         7132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_680249, whole genome shotgun sequence.
ACCESSION   DATBSZ010000311 DATBSZ010000000
VERSION     DATBSZ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7132
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680249"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VIG70_20170"
     CDS             <1..570
                     /locus_tag="VIG70_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8252916.1"
                     /translation="NDANCFALSEAVDGAGKGAGVVFGVILGTGVGGGVVVDGKVLVG
                     ANAIAGEWGHNPLPAPRAADLPHPACYCGRTGCIETYLSGPGLSRDHESRTGRRLSPE
                     EIVSLGDQSLERYEERLARALASVINLLDPDVIVLGGGMSNVARLYTEVPRLWGRHVF
                     SDRVATRLARPVHGDSSGVRGAAWLWGKN"
     gene            587..1372
                     /locus_tag="VIG70_20175"
     CDS             587..1372
                     /locus_tag="VIG70_20175"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEY8252917.1"
                     /translation="MKLQLVALSAALASNAAFAGPEKIKFPSDYLKGVLYQTLDRPDN
                     KQYRELYATPAAVEAVRKGQPIPSGSVLTLVQWSVEQDSSGNPIKDSNGRYIKKDIIA
                     HTVMEKRTGWGADYPSDWPRNGEWEYAVFTADGQPNPKGNANNKACFTCHLPHAKQDF
                     VISLAKLNNTFPGSQTLVKGIKGDVNIASFAFMPAKISAMAGKALTFFNGDDTPHQIT
                     VAGGPRTEVFLRGQKASLTIDKPGEYNYICGLHPSMKGVIEVK"
     gene            1460..3070
                     /locus_tag="VIG70_20180"
     CDS             1460..3070
                     /locus_tag="VIG70_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252918.1"
                     /translation="MKDTLSAVAASAALALAVAGCATQESHEGHARLGKVNFPVECNA
                     EAQREFNLAMAYYHSFAWQHVREPLEGVLKADPSCGMAHWARALASLDNPFTWPGTIS
                     VATLNQGPDILETARKTGLKSQRERDYVDALGVFFRDHDKLDHRTRARALENALDQVR
                     SRYPQDSEAAILYALVLSANFDPTDRKYTNQLKAAAVLEPIFKQQPEHPGVAHYLIHS
                     YDYPPIAKQGLDAARRYDKIAPDAPHALHMPSHIFTRVGAWKESIRSNRASATSATDK
                     TFDKWHAYDYMVYAHLQLGEDGAARKVVAEALGNAARVDHFATAYAYAAMPARLALER
                     AAWKDAAKVELAPAAAAYPWAKYPQAESINAYARGIGAAMSGDAAGARVELARLEKLR
                     GVAVERKIGYWAEEIGIQAEVVRGLSFCAEGKRADCAATLRAAADREDKLEKHVVTPG
                     RLVPAREMLAAVTLESGDAAAALREYETTLEREPNRLRTFAGAARAAERAGDKAKGAQ
                     YARRVVEQTASADSQLVDVADAKRLLGR"
     gene            complement(3056..4312)
                     /locus_tag="VIG70_20185"
     CDS             complement(3056..4312)
                     /locus_tag="VIG70_20185"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8252919.1"
                     /translation="MTTSSQSVLILGCGFGGLWAAQALRKAPVEVTVLDRTNHHLFTP
                     LLYQVATAGLSAPSVAGPIRHILAHQRNTTVLLADAQAVLPAEKCVLSENGDKIPYDY
                     LIVATGTTHSYFGHDDWAPYAPGLKTLADALEIRRRVLLGFELAERETDPARRAAWLT
                     FVVVGGGPTGVELAGTFAEIARHTLRGEFRRIDPHSARIVLVEGTGRVLPAYPADLSR
                     KAQLQLERLGVTVWPGRLVTGVDADGIQIGAERLSAKTVVWAAGVAASPLGRSLGAPL
                     DRAGRVRVAPDLSVPGHPEIFVAGDLASVDGVPGIAPAAKQMGSHAAKNIVLSLKGEK
                     TRPFRYRDYGQLATIGRNAAVVEMGKMQLSGFPAWLVWLVAHIYFLINFRNRLIVLID
                     WGWAYFTYQRYARIMIRPGPDRSSPK"
     gene            4340..5038
                     /locus_tag="VIG70_20190"
     CDS             4340..5038
                     /locus_tag="VIG70_20190"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8252920.1"
                     /translation="MKTVYGQFCPVAVASEIFAERWTPVILRELVLGSRRFNDIHRGV
                     PRISRGLLAKRLRELTASGVIENTDGDYHLTHAGEELGEVVKSLGAWGARWTAPVQRE
                     RLDARLLTWDMRRRFDVERLPEKRVTLRFDFRGVPQGHKAPRTYWFVLQRPEVDLCII
                     EPGFEVDLFVDADLATFVRVWLGEVPMRQVIREGAIKLAGAREAVRDFPSWLLLSTLA
                     AVPRPAESIQRRVA"
     gene            5035..6132
                     /locus_tag="VIG70_20195"
     CDS             5035..6132
                     /locus_tag="VIG70_20195"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8252921.1"
                     /translation="MNARADWRATLAALTPAFAERAARLDESDEFVAENYRELRASGL
                     LAAGVPKELGGEGLELEELCEMLRTLAQACPSTALAFSMHTHQVAVAAWRLKHQKAPV
                     TPMLEKVARDRVIILTTGGGDWLDSSGEAVAVEGGFRIKARKPFASASPVGDLLSTSA
                     VLGSEVIHFVIPTKTQGVRIEPTWRTMGMRNTASHDVVLEDVFVPESGVAIRRPRGKW
                     HPLFHLLSIIALPLIYAVYLGVAEAARERALALVRKRRPDDHLFQAVGEMENQLAAAR
                     LAHADWVALGKGAQPGPETTSKAMIDRTLAARGVLGTVDAAMNVAGGAAFFRVNGLER
                     LFRDAQGARYHPLQEGLQKTLTARHALGLEI"
     gene            complement(6134..7009)
                     /locus_tag="VIG70_20200"
     CDS             complement(6134..7009)
                     /locus_tag="VIG70_20200"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY8252922.1"
                     /translation="MMQTMQISKLKESFGVAIEGVSLASLDERGFSEIHDLWKAHGAM
                     LVRGQQAMSDAQFEAFSKRFGELDPPPNQGVGRKNVPGFPNLYVVSNELDAGGEPLGA
                     LGYSEAVWHTDMSYLPVPPIASMLLARKLPAWGGNTWAASMCAAYDNLPAKLKERIRT
                     LKIKHDGTRDSGGNLRKGVEDDPNPVTSRGHPHPAVIRDPGTGKPALFLGRRPRAYVM
                     GLPVEESEALLDELWRYAFKPANTYEHHWQVGDVLIWSNYSTMHKRDEFDKATIRRMH
                     RSQIKGTAAPAAYFS"
BASE COUNT         1165 a         2381 c         2517 g         1069 t
ORIGIN      
        1 aacgacgcca actgctttgc cttgtcggaa gcggtggacg gagcggggaa gggcgcgggc
       61 gtggtgttcg gcgtgatcct cggcaccggg gtcggcggcg gggtcgtggt cgacggcaag
      121 gtgctggtcg gggcgaacgc cattgccggg gaatgggggc acaatcccct gccggcgccg
      181 cgcgccgcgg atttgccgca tccagcctgc tactgcggcc gtacaggctg tatcgagact
      241 tacctgtcag gtccgggcct gtcgcgcgac catgagagca gaacgggaag gcggctgtcg
      301 ccggaggaaa tcgtttctct tggggatcag tctcttgaga ggtacgagga acgcctggcg
      361 cgggcgctcg cgtcggtgat caacctgctc gacccggacg tgatcgtgct gggcggcgga
      421 atgtcgaacg tggcgcggct gtacactgaa gtgccgcgcc tatgggggcg gcacgtgttc
      481 tccgaccggg tggcgacgcg gcttgcgcgt ccggtgcacg gcgactcgag cggggtgcgc
      541 ggggcggcct ggctttgggg aaagaactga acgaaaggga gatctcatga aactgcagct
      601 cgtcgcactg tccgcagcgc tcgcatccaa cgcagccttc gccggacccg agaagatcaa
      661 gtttccgtcc gactacctga agggcgtgct ctaccagacg ctcgaccggc cggacaacaa
      721 gcagtaccgc gagctctatg ccacgccggc ggctgtcgag gcggtacgca agggccagcc
      781 gatcccgagc ggcagcgttc tcacgctggt gcagtggagc gtggagcagg actcgagcgg
      841 caacccgatc aaggactcga acggccgcta tatcaagaag gacatcatcg cccacacggt
      901 gatggagaag cgcaccggct ggggcgccga ctatccgtcc gactggccgc gcaatggcga
      961 gtgggagtac gcagtgttca ccgccgacgg ccagcccaac cccaagggca acgccaacaa
     1021 caaggcctgc tttacctgcc acctgccgca cgccaagcag gacttcgtga tctcgctggc
     1081 gaagctgaac aacacgttcc cgggctctca gacgctcgtc aaaggaatca agggcgacgt
     1141 caatatcgcg tcgttcgcct tcatgccggc gaagatcagc gcgatggcgg gcaaggcgct
     1201 caccttcttc aacggtgacg acacgccgca ccagatcacg gtggcgggcg ggccgcggac
     1261 cgaggtgttc ctgcgcggcc agaaggcttc cctcaccatc gacaagcccg gcgagtacaa
     1321 ctacatctgc gggctgcacc cgagcatgaa gggcgtgata gaggtgaaat gaggcagtga
     1381 aataggggcc cgcggttcag atcctgaact tgcggcggcc gcgcgagggg cgcaagctgg
     1441 tcgcatcagg aggcaaaccg tgaaagacac gctatccgca gtggcggcga gcgccgccct
     1501 cgcgctcgca gtcgcaggct gcgccaccca ggagtcgcac gaaggccatg cgcggctcgg
     1561 caaggtgaat ttcccagtgg agtgcaatgc cgaggcgcag cgcgagttca acctcgcgat
     1621 ggcgtactac cattcgttcg cctggcagca tgtccgcgag ccgctcgagg gcgtgctcaa
     1681 ggcggacccg agctgcggca tggcgcactg ggcgcgagcg ctcgcgagcc tcgacaaccc
     1741 cttcacctgg ccgggcacca tctcggtcgc gacgctgaac caggggcccg acatcctcga
     1801 gacggcgcgc aagaccgggc tcaagtccca gcgcgagcgc gactacgtcg acgcgctagg
     1861 cgtgttcttc cgcgaccacg acaagctcga ccaccgcact cgcgcccggg cgctggaaaa
     1921 cgcgctcgac caggtccgca gccgctatcc gcaggacagc gaggccgcga ttctctatgc
     1981 gctggtcctg tcggccaact tcgatcccac ggaccgcaag tacaccaacc agctcaaggc
     2041 ggcggcggtg ctcgagccga tcttcaagca gcaacccgag catcccgggg tggcgcatta
     2101 cctcattcac agctacgact atccgccgat cgcgaagcag ggcctcgacg ccgcgcggcg
     2161 ctacgacaag atcgcgccgg acgcgccgca cgcgctgcac atgccctcgc acatcttcac
     2221 gcgggtcggg gcctggaagg agtcgatccg ctccaaccgc gcatcggcca cctcggccac
     2281 cgacaagact ttcgacaagt ggcacgccta cgactacatg gtgtacgccc acctgcagct
     2341 cggcgaagac ggcgccgcgc gcaaagtcgt cgcggaggcg ctcggcaatg ccgcgcgcgt
     2401 cgatcacttc gccaccgcct acgcctatgc ggcgatgccg gctcgcctgg cgctggaacg
     2461 cgcggcgtgg aaggacgccg cgaaggtgga gctcgccccc gcggccgcgg cctacccgtg
     2521 ggccaagtat ccgcaggccg agtcgatcaa cgcctacgcg cgcggcatcg gcgcggcgat
     2581 gagcggcgat gcggcaggcg cgcgcgtcga gctcgcgcgg ctcgagaagc tgcgcggggt
     2641 cgcggtagag cgcaagatcg gctactgggc ggaagagatc ggcatccagg ccgaggtggt
     2701 gcgcggcctc tccttctgcg cggaaggcaa gcgagccgac tgcgccgcca ccctgcgcgc
     2761 ggcggcggat cgcgaggaca agctcgagaa gcacgtcgtc acgcccggtc gattggtccc
     2821 ggcacgcgag atgctcgccg ccgtcacgct cgaatccggc gacgccgcgg ccgcgctgcg
     2881 cgagtacgaa accacgctcg agcgcgagcc gaaccgcctg cgtaccttcg ccggcgcggc
     2941 acgcgcggcg gagcgcgccg gggacaaggc gaagggagca cagtacgccc ggcgcgtcgt
     3001 cgagcagacc gcaagcgccg acagtcagct cgtcgacgtc gccgacgcca agcggctact
     3061 tgggagatga ccggtcgggt ccgggccgga tcatgatgcg cgcgtagcgc tggtaggtga
     3121 agtacgccca cccccagtcg atcagcacga tcaggcgatt gcggaagttg atgaggaagt
     3181 agatgtgggc gaccagccag accagccacg ccgggaagcc cgacagctgc atcttgccca
     3241 tctccaccac ggcggcgttg cggccaatgg tggcgagctg gccgtagtcg cggtagcgaa
     3301 acggcctcgt cttctcgcct ttcagggaaa gcacgatgtt ttttgccgca tggctcccca
     3361 tctgcttcgc cgcgggtgcg atgcccggca cgccgtcgac cgaggcgagg tcgccggcga
     3421 cgaagatttc cggatgaccc ggcactgaaa ggtcgggcgc gacgcgcacg cgcccggcgc
     3481 ggtcgagcgg cgcgcccagg ctccggccga gcggcgaagc ggcaaccccc gcggcccaga
     3541 cgacggtctt ggcggaaagg cgctcggcgc cgatctggat gccatccgcg tcaaccccgg
     3601 taacaagccg gccgggccag accgtgaccc ccaggcgctc gagctggagc tgcgccttgc
     3661 gcgacaggtc cgccgggtag gcgggcagca cgcggccggt tccctcgacc agcacgatgc
     3721 gcgcgctgtg cgggtcgatg cggcggaact cgccgcgcag cgtatggcgg gcgatttcgg
     3781 cgaaggtgcc ggcgagctcg accccggtcg ggccgccgcc gacgaccacg aaggtgagcc
     3841 aggcggcgcg ccgcgcgggg tcggtctcgc gctcggcgag ctcgaagccg agcagcaccc
     3901 tgcggcggat ctcgagcgcg tcggccagcg tcttcagccc gggcgcatac ggcgcccagt
     3961 cgtcgtggcc gaaatagctg tgcgtcgtgc cggtggcgac gatcaggtag tcgtagggaa
     4021 ttttgtcgcc attttcggat aggacgcact tctccgcggg aagaacggct tgagcatcag
     4081 ccagcagcac cgtggtgttg cgctggtggg cgaggatgtg gcggatcggg ccggctaccg
     4141 agggcgccga caggcccgcg gtcgccacct gatagaggag cggcgtgaac aggtggtggt
     4201 tggtgcggtc gagcaccgtg acctccaccg gcgccttgcg cagcgcctgc gccgcccaca
     4261 ggccgccgaa gccgcagccg aggatcagta cagattgtga acttgtcgtc atgccgcgcg
     4321 aggtctaggg tacgtgtcca tgaaaaccgt ctacggccag ttctgtccgg tcgcggtcgc
     4381 gagcgaaatc ttcgccgagc gctggacgcc cgtcatccta cgcgagctcg tgctcggcag
     4441 ccgccgcttc aacgacatcc accgcggcgt gccgcgcatc tcgcgggggc tgcttgccaa
     4501 gcggctgcgc gagctgaccg cgtccggggt gatcgagaac acggacggcg actatcacct
     4561 gacgcatgcc ggcgaggagc tgggcgaggt ggtcaagagc ctcggcgcct ggggcgcgcg
     4621 ctggaccgcg ccggtgcagc gcgagcgcct ggatgcccgc ctgctgacct gggacatgcg
     4681 ccggcgcttc gacgtcgagc gcctgcccga gaagcgcgtc acgctgcgct tcgatttccg
     4741 cggcgtgccg caggggcaca aggcgccgcg cacctactgg ttcgtgctgc agcgcccgga
     4801 agtggacctg tgcattatcg agccgggctt cgaagtcgac cttttcgtcg atgcggatct
     4861 cgccacgttc gtccgggtgt ggctcggcga ggtgccgatg cgccaggtga tccgcgaagg
     4921 cgcgatcaag cttgccggcg cgcgcgaggc ggtgcgcgat tttccgtcgt ggttgctgct
     4981 cagcacgctg gccgccgtgc cgcggccggc cgaatcgatc caaaggagag tggcatgaac
     5041 gcaagagcag attggcgtgc gacgctggcg gctttgacgc cggccttcgc cgagcgcgcc
     5101 gcgcggctgg acgagagcga cgaattcgtc gcggagaact accgcgagct cagggcgagc
     5161 gggctgctcg cggcgggcgt gccgaaagag ctgggcggcg agggcctcga gctcgaggag
     5221 ctgtgcgaga tgctgcgcac cctggcgcag gcctgcccga gcaccgcgct cgcgttctcg
     5281 atgcacacgc accaggtggc ggtcgccgcc tggcgcctga agcaccagaa ggcgccggtc
     5341 acgccgatgc tggagaaggt ggcgcgcgac cgggtgatca tcctcaccac cggcggcggc
     5401 gactggctcg acagctcggg cgaagcggtt gcggtcgagg gcggctttcg catcaaggcg
     5461 cgcaagccgt ttgcgagcgc ctcgcccgtc ggcgacctgc tctccacatc cgcagtgctc
     5521 ggcagcgagg tcatccactt cgtcatcccg accaagaccc agggcgtgcg tatcgaaccg
     5581 acctggcgaa cgatggggat gcgcaatacg gcgtcgcacg atgtcgtgct cgaggatgta
     5641 ttcgtgccgg agagcggggt ggcgatccgc aggccgcgcg gcaagtggca tccgctgttc
     5701 catcttctga gcatcatcgc gctgccgctg atctacgcgg tctatctcgg cgtcgccgag
     5761 gcggcgcgcg agcgcgcgct ggcgctcgtg cgcaagcgcc ggccggacga tcacttgttc
     5821 caggcggtcg gcgaaatgga gaaccagctt gccgcggcgc gcctggcgca cgccgactgg
     5881 gtggcgctcg gcaagggggc acaacccggg ccggagacca cgagcaaggc gatgatcgac
     5941 cgcacgctcg ccgcgcgcgg cgtgcttggc accgtagacg ccgccatgaa cgtggcgggc
     6001 ggcgcggcgt tcttcagggt caacggcctg gagcggctgt tccgcgacgc gcagggcgcg
     6061 cgctatcacc cgctgcagga gggcctgcag aagaccctca cggcgcggca cgcgctcgga
     6121 ctggagatct aggctaggag aagtaagccg cgggagcggc ggtgcccttg atctggctgc
     6181 ggtgcatgcg gcggatggtg gccttgtcga actcgtcgcg cttgtgcatc gtgctgtagt
     6241 tgctccagat cagcacgtcg cccacctgcc agtggtgctc gtaggtgttc gccggcttga
     6301 acgcgtagcg ccacagttcg tcgagcagcg cctcggactc ctcgacgggc agccccatca
     6361 cgtaggcgcg cgggcgccgg ccgaggaata gcgcgggctt tcccgtgccc gggtcgcgga
     6421 tcaccgccgg gtgcggatgg ccccgggagg tcaccgggtt cgggtcgtcc tcgacgccct
     6481 tcctcaggtt gccgccggaa tcgcgcgtgc cgtcgtgctt gatcttgagc gtcctgatgc
     6541 gttccttcag cttggcgggc aggttgtcgt acgcggcgca catgctcgcc gcccacgtgt
     6601 tgccgcccca ggcgggcagc ttgcgcgcga gcagcatcga ggcgatcggc ggcaccggca
     6661 ggtagctcat gtccgtgtgc cagaccgcct cgctgtagcc gagcgcgccg agcggctcgc
     6721 cgccggcatc gagctcgttc gacaccacgt agaggttcgg gaagcccgga acgttcttgc
     6781 gccccacgcc ctggttgggc ggcgggtcga gctcgccgaa gcgcttcgag aacgcctcga
     6841 actgcgcgtc gctcatcgcc tgctggccgc gcaccagcat cgcgccatgc gctttccaca
     6901 ggtcatggat ctcggaaaag ccccgctcgt cgagcgacgc gaggctgacg ccttcgatgg
     6961 cgacgccgaa gctctctttc agcttgctga tctgcatcgt ctgcatcatt cggcctcgat
     7021 gttcgcgtgc ttcaccaccg cgcgccattc ggcgatctcg cgcttcacca tctgtccgag
     7081 ccgctcgggc gtggagctca tgccgtcggc gccctgctcg agcagcttcg cc
//
LOCUS       DATBSZ010000312         6307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1977993, whole genome shotgun sequence.
ACCESSION   DATBSZ010000312 DATBSZ010000000
VERSION     DATBSZ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977993"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VIG70_20205"
     CDS             complement(<1..422)
                     /locus_tag="VIG70_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY8252923.1"
                     /translation="MHAFEYHRPGSTKDALALGKNEEARYLAGGQSLVQAMKLRLSSP
                     SDLVDLGTIKELVGIKVSSGSVEIGAMTRHADVEGSKDVQKAIPALAALAGIIGDRQV
                     RHMGTLGGSLANSDPAADYPAAVLGLGATITTNKRKIEA"
     gene            complement(409..2766)
                     /locus_tag="VIG70_20210"
     CDS             complement(409..2766)
                     /locus_tag="VIG70_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8252924.1"
                     /translation="MNAPLIGARVLRKEDYRFLTGTGQYTDDVALPRQTYAAFVRSPH
                     AHARIKSINSDKAKKSPGVLAVFTGADLAAAKVGGLPCGWLITDVNGKPMKEPPYPPL
                     VQGKALHVGDRVAVVIAETLEQARDAADKVKVDYEELPAVVDAAKARKGPALHDIAPD
                     NTCYVWALGDKAAVDGAFSKAHHVTKLDFVNNRLVPNAIEPRAANATYSRADDSYTLY
                     VASQNPHVERLLMTAFVLGLPESKVRVVAPDVGGGFGSKIYLYGEDVVVTWAAKQLNR
                     PVKWTADRSESYVSDAHGRDHVTTAELALDKDGNFLAMRVKTTANLGAYLSTFASCIP
                     TILYATLLAGQYKTPLIHCEVTAVFTNTTPVDAYRGAGRPEATYVVERLVETAAREMK
                     MDPVEIRRRNFIKNFPYQTPVALCYDTGDYEATLSAALKLADVSGYPRRKEESLKKGK
                     LRGIGYACYIEACGIAPSNVAGSLGARAGLFEAGEIRVHPTGTVTVFTGSHSHGQGHE
                     TSFAQVVADRLGLALEQVEIVHGDTSKVLFGMGTYGSRSIAVGGTAIVKALDKIIAKG
                     KKIAAHLLEASEADIEYDRGQFKVAGTDRKKMFGEVAFAAYVPHNYPLDKLEPGLNEN
                     AFYDPTNFTFPAGTYVCEVEVDKDTGATQIAAFTAVDDFGKIINPMIVEGQVHGGLAQ
                     GIGQALTEGCRYESSSGQLVTGSFMDYCIPRSSDVPLFKIDTRETPCTHNPLGVKGCG
                     EAGAIGAPAAVMNAVTDALGVKDVAMPATSQTVWKHLQEKSHARV"
     gene            complement(2763..3242)
                     /locus_tag="VIG70_20215"
     CDS             complement(2763..3242)
                     /locus_tag="VIG70_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8252925.1"
                     /translation="MTIKVSMKVNGKPVSAEVEARTLLVTYIRENLRLTGTHVGCDTA
                     QCGACTVLMNGRAIKSCSIVAMQAEGADLMTIEGVAKPDGTLHPMQQAFKENHGLQCG
                     FCTPGMVMNALDFAKSHPNPTDQEVREALEGNLCRCTGYHNIVKSIVAGAKAMGGKL"
     gene            3316..3996
                     /locus_tag="VIG70_20220"
     CDS             3316..3996
                     /locus_tag="VIG70_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8252926.1"
                     /translation="MRLLIVEDDAPLASGLQRILEAEGHAVDVTPRGEDAVAAASQEK
                     FDLVILDIGLPQMDGFEVLRRLRGTASDKTGPTPVLVLTARDAVDDRVRGLDLGADDY
                     MVKPFAMPELTARVRALLRRSQAHGGPRITHGPLTLDTVARRAFLRGEPLELAAREWA
                     VLEVLLAKVEKIVSKEAIIQAVAGWGDDLSPNAIEVYVSRLRSKLEPAGIRIRTVRGF
                     GYMLEEFK"
     gene            4001..5338
                     /locus_tag="VIG70_20225"
     CDS             4001..5338
                     /locus_tag="VIG70_20225"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8252927.1"
                     /translation="MAGRSLRAHLLRLLLPPIAALLAVGAVVAYYPSIEPATQAYDQA
                     LVDIGLALGSHVRVGQSEYRFDLPPAVEQVLRTDRFDSIYYRVISPAGLEIAGDADLP
                     APPGDAIAHNAVHRGQKVRVVSVQTSCGRSACNVLVAETMVKREHLARDLLLQSLFPD
                     FLIALATLVIVWFGVKRGLEPLARLSDEIKARSAGDLRPIDAAGAPEETLPLVGALNG
                     LLDEVSAASRKQQRFLADAAHQLRTPLAGLQAHTELALAQPLPEGCRAQLEQVHRATI
                     RTARLANQLLALARAEPGARSVTSSVDLKGIAGGEADAWVRQSLGRDIDLGFELEPAL
                     VEGDAFLLREALSNLVHNALEYSNRGGRVTVRTGRVNGHAFLEVEDDGPGIAQEERAR
                     VLERFYRVPGTPGTGSGLGLAIVREIAASHAASMFIADGSAGGCRVGITFPHG"
     gene            5331..>6307
                     /locus_tag="VIG70_20230"
     CDS             5331..>6307
                     /locus_tag="VIG70_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY8252928.1"
                     /translation="MDSVDIEVLRSAEAWRKSGHKVALGTIVKTWGSAPRPIGAMVAV
                     REDGQVVGSVSGGCVEDDLIDKVKAKLAGWKKPELFTYGVTNEDATRWGLPCGGTLQL
                     VMEPLSERSGVQELLQRISSQQLVKRRLEMDSGSSTLEPGRWQDVLEFDGRVLSTVHG
                     PRWRLVLIGAGQLTRYLAEMAHMLDYHVVVIDPREEYAAGWDLASAPLNRGMPDDVVR
                     ELQLDGHSALVALTHDPKLDDLALMEGLKSAAFYVGAIGSKKNNDARRERLKEFDVSA
                     DEIARLRGPVGLYIGSKTPPEIALAILAEMTAVRHGVREPSWAAKPERR"
BASE COUNT          926 a         2127 c         2187 g         1067 t
ORIGIN      
        1 gcctcgatct tgcgcttgtt ggtggtgatg gtcgcgccaa gcccgagcac tgccgcggga
       61 tagtcggccg ccgggtcgct gttggcgagc gagccgccga gggtgcccat gtggcgcacc
      121 tggcggtcgc cgatgatgcc ggcgagcgcg gcgagcgccg ggatcgcctt ctgcacgtcc
      181 ttggagccct cgacgtcggc gtggcgcgtc atcgcgccga tctcgacgga accgctcgag
      241 accttgatac cgaccagctc cttgatcgtt cccaggtcga ccagatcgct gggagaagaa
      301 aggcggagct tcatcgcctg caccaggctc tgcccgcccg cgaggtacct ggcctcttcg
      361 ttcttgccca gggcgagcgc gtccttcgtg ctgccgggcc ggtggtattc aaacgcgtgc
      421 atggcttttc tcctggagat gcttccaaac cgtttgcgag gtcgccggca tggcgacgtc
      481 cttcacgccg agcgcgtcgg tgacggcgtt catcaccgcc gccggcgcgc cgatcgcgcc
      541 ggcttcaccg cagcccttga cgccgagcgg gttatgcgtg cacggcgtct cgcgcgtatc
      601 gatcttgaac agcggcacgt ccgacgagcg cgggatgcaa tagtccatga acgagccggt
      661 caccagctgc cccgaggagg attcgtagcg gcagccctcg gtcagcgcct ggccgattcc
      721 ctgcgccagc ccgccgtgca cctggccctc gacgatcatc gggttgatga tcttgccgaa
      781 atcgtcgacc gcggtgaacg cggcgatctg cgtcgcgccg gtgtccttgt ccacttccac
      841 ctcgcacacg taggtccccg cggggaaggt gaagttggtc gggtcgtaga acgcgttctc
      901 gttcagcccg ggctcgagct tgtcgagcgg gtagttgtgc ggcacgtagg ccgcgaacgc
      961 cacctcgccg aacatcttct tccggtcggt gcccgcgacc ttgaactggc cgcggtcgta
     1021 ctcgatgtcg gcctcggagg cttccagcag gtgcgcggcg atcttcttgc ccttcgcgat
     1081 gatcttgtcg agcgccttca cgatcgcggt gccgccgacc gcgatcgagc gcgagccgta
     1141 ggtgcccatg ccgaacagga ccttcgaggt gtcgccgtgc acgatctcga cctgctcgag
     1201 cgccaagccg agccggtcgg cgaccacctg cgcgaagctg gtctcgtggc cctggccgtg
     1261 cgagtgcgag ccggtaaaca ccgttacggt cccggtcgga tgcacccgga tctctcctgc
     1321 ctcgaacagg cccgcgcgcg cgcccaggct gccggcgacg ttcgagggcg cgatgccgca
     1381 ggcctcgatg tagcaggcat acccgattcc ccgcagtttt cctttcttga gagactcctc
     1441 ttttcgccgc gggtagccgg acacgtcggc cagtttcaag gcggcgctca aggtggcctc
     1501 gtagtcgccg gtgtcgtagc acagcgcgac cggcgtctgg tacggaaaat tcttgatgaa
     1561 gttgcgccgc cggatttcca ccggatccat tttcatctcg cgcgcggcag tctccaccag
     1621 ccgctcgacg acgtaagtgg cttccggccg gccggcgccg cggtaggcgt ccaccggcgt
     1681 ggtgttggta aacaccgcgg tcacctcgca gtggatgagc ggcgtcttgt actgcccggc
     1741 gagcagcgtc gcgtacagga tggtcggaat gcacgaggcg aacgtggaga gatacgcgcc
     1801 gaggttggcg gtcgtcttca cgcgcatggc gaggaaattg ccgtccttgt cgagcgcgag
     1861 ctcggcagtc gtgacgtggt cgcgcccgtg cgcgtcggac acataggatt ccgagcgatc
     1921 ggcggtccac ttcaccggcc ggttcagctg cttcgctgcc caggtgacca cgacgtcttc
     1981 gccgtacaga tagattttcg agccgaagcc gccgccgacg tcgggggcga ccacgcgcac
     2041 cttgctctcc ggcaggccca gcacgaacgc ggtcatcagc agccgctcga cgtgcgggtt
     2101 ctggctcgcg acgtagagcg tgtagctgtc gtctgcgcgc gagtaggttg cgttcgccgc
     2161 gcgcggctcg atcgcgttgg gaacgagccg gttgttgacg aagtcgagct tcgtaacgtg
     2221 atgtgctttc gaaaaagcac catcgactgc ggccttgtcg cccagcgccc agacgtagca
     2281 ggtattgtcc ggcgcgatgt cgtggagcgc cgggcccttg cgcgccttgg cggcgtcgac
     2341 taccgcgggt agttcctcat agtcgacctt gaccttgtcg gcggcgtcgc gcgcctgctc
     2401 gagggtctcg gcaatgacca ccgcgacgcg atcgccgacg tgcaacgcct tgccttggac
     2461 cagcggcggg tacggcggct ccttcatcgg cttgccgttc acatccgtga tcagccagcc
     2521 gcacggcagt ccgccgacct tcgccgcggc caggtccgcg cccgtaaaga cggcaaggac
     2581 cccgggagat ttctttgcct tgtcgctgtt gatgctcttg atgcgggcat gcgcgtgcgg
     2641 cgagcgaaca aaggccgcat aggtctgcct aggcagcgcc acatcgtcgg tgtactggcc
     2701 ggtgccggtg aggaagcggt agtcctcctt gcgcaggacg cgcgcgccga tgagcggggc
     2761 gttcacagtt tgcctcccat cgccttcgcg ccggccacga tgcttttcac gatgttgtgg
     2821 tagccggtgc agcggcagag attcccctcg agcgcctcgc gcacctcctg gtcggtcggg
     2881 ttcggatggc tcttcgcgaa gtcgagcgcg ttcatcacca tgcccggggt gcagaagccg
     2941 cactgcaggc catggttctc cttgaacgcc tgctgcatcg ggtgcagggt gccgtcgggc
     3001 ttcgccaccc cctcgatggt catcaggtcg gcgccttctg cttgcattgc aacaatcgag
     3061 cagctcttga tggcgcggcc gttcatcagg acggtgcagg cgccgcactg cgcggtgtca
     3121 cagccgacgt gggtgccggt aagcctaagg ttttcgcgga tataggtgac gaggagggtg
     3181 cgcgcctcga cttccgcgga aacgggcttg ccgttgactt tcatggatac cttgatggtc
     3241 atgcgctcct cctgggggaa aaatatccta gcgcggcggt atcatttgcg ccacacccat
     3301 ttctggccgc tcgccatgcg attgctgatc gtggaggacg acgcgcccct tgcctcagga
     3361 ctgcagcgca tcctcgaggc cgagggccac gcggtcgacg tgacgccgcg cggcgaggac
     3421 gcggtggcgg ccgcgtcgca ggaaaagttc gatctcgtga tcctggacat cgggctgccg
     3481 cagatggacg gtttcgaggt gctgcggcgg ctgcgcggca cggcgtcgga caagaccggg
     3541 ccgacgccgg tcctggtgct caccgcgcgc gacgccgtgg acgatcgcgt gcgcgggctc
     3601 gatctgggcg ccgacgacta catggtcaag cccttcgcca tgccggagct gaccgcgcga
     3661 gtgcgcgcgc tcctgcggcg ctcgcaggcg cacggcgggc cgcgcatcac ccacgggccg
     3721 ttgacgctcg atacggtggc gcggcgcgct ttcctgcgcg gcgagccgct cgagctcgcg
     3781 gcgcgcgaat gggcggtgct cgaagtgctg ctcgccaagg tggagaagat cgtgtcgaag
     3841 gaggccatca tccaggcggt cgcgggctgg ggcgacgacc tctcgccgaa cgcgatcgag
     3901 gtgtacgtct cgcggctgcg ctcgaagctc gagcccgccg gcatccggat ccgtaccgtg
     3961 cgcggcttcg gctacatgct ggaggagttc aagtaagcgc atggccgggc gaagcctccg
     4021 cgcccacctg ctgcgcctgc tgctgccgcc gatcgccgcc ttgctcgcgg tcggggcggt
     4081 ggtcgcctat tacccgtcga tcgagccggc cacccaggcc tacgaccaag ccctcgtcga
     4141 catcggtctc gcgctcggct cgcacgtccg cgtcggccag agcgaatacc gcttcgacct
     4201 gccgccggcg gtcgagcagg tgctgcgcac cgatcgcttc gacagcatct attaccgcgt
     4261 gatcagcccg gcagggctcg agatcgccgg cgacgccgac ctgccggcgc cgccgggcga
     4321 cgcgatcgcc cacaacgccg tgcacagggg ccaaaaggtg cgcgtggttt cggtgcagac
     4381 gtcgtgcggc cggtccgcgt gcaacgtgct ggtcgcggag accatggtca agcgcgagca
     4441 cctcgcccgc gatctcctgc tgcaaagcct gttccccgat ttcctgatcg cgctcgccac
     4501 cctggtcatc gtgtggttcg gcgtgaagcg cggcttggag ccgctcgcgc gcctctccga
     4561 cgagatcaag gcgcgctcgg ccggcgacct gcggccgatc gacgccgccg gcgcccccga
     4621 ggagacgctg ccgctggtcg gcgccctgaa cgggctgctc gacgaagtgt cggcggcgag
     4681 ccgcaagcag cagcgctttc tcgccgacgc cgcgcaccag ttgcgcacgc cgctcgccgg
     4741 gctgcaggcg cacacggagc tcgcgctcgc gcagcccctg ccggagggct gccgcgcgca
     4801 gctcgagcag gtgcaccgcg cgacgatccg caccgcgcgc ctcgccaacc agctcctggc
     4861 gctggcgcgc gcggagccgg gcgctcgctc ggtgacttcc agcgtcgacc tgaagggcat
     4921 cgcgggcggc gaagccgacg cctgggtacg ccagtcgctc gggcgcgaca tcgacctcgg
     4981 attcgagctc gagcccgcgc tggtggaggg cgacgctttc ctgctgcgcg aggcgctctc
     5041 caatctcgtg cacaacgcgc tcgagtactc caaccgcggc gggcgcgtca ccgtgcgcac
     5101 cgggcgcgtg aacggccatg cctttctcga agtcgaggac gacgggccgg gaatcgcgca
     5161 ggaagagcgc gcgcgcgtgc tcgagcggtt ctaccgcgtg ccgggcaccc cgggcaccgg
     5221 cagcggcctg ggcctcgcga tcgtacggga gatcgccgcg agtcacgcgg cgagcatgtt
     5281 catcgccgac ggatcggctg gcggctgcag agttgggata acatttcccc atggatagcg
     5341 tagacatcga agtcctgcgt tccgcggagg catggcgcaa gagcggtcac aaggtcgcgc
     5401 tcggaacgat cgtcaagacc tggggctcgg cgccgcgccc gatcggcgcg atggtggcgg
     5461 tgcgcgagga cggccaggtg gtgggctcgg tgtccggcgg ctgcgtcgag gacgatctga
     5521 tcgacaaggt gaaggccaag ctcgccggct ggaagaagcc cgagctcttc acctacggcg
     5581 tgaccaacga ggacgcgacg cgctgggggc tgccgtgcgg cggcacgctc cagctcgtga
     5641 tggagccgct ctccgagcgc agcggcgtgc aggagctgct gcagagaatc agctcccagc
     5701 agctcgtgaa aaggcggctc gagatggaca gcggcagctc gacgctcgag cccggacgct
     5761 ggcaggacgt gctcgagttc gacggccggg tgctctccac ggtgcacggc ccgcgctggc
     5821 gcctggtgct gatcggcgcg ggccagctca cccgctacct cgccgagatg gcgcacatgc
     5881 tcgactacca cgtggtggtg atcgacccgc gcgaggagta cgccgcgggc tgggacctcg
     5941 cctcggcgcc gctcaaccgc ggcatgcccg acgacgtggt gcgcgagctg cagctcgacg
     6001 gccacagcgc gctggtcgcg ctgacccacg acccgaagct cgatgacctg gcgctgatgg
     6061 aagggctcaa gtcggcggcg ttctacgtcg gcgcgatcgg ctcgaagaag aacaacgacg
     6121 cgcgccgcga gcggctgaag gagttcgatg tctccgccga cgagatcgcg cgcctgcgcg
     6181 gcccggtcgg gctctacatc ggctccaaga ccccgccgga gatcgcgctc gcgatcctcg
     6241 ccgagatgac cgcggtgcgc cacggcgtga gggagccctc gtgggcggcg aagcccgagc
     6301 ggcgctt
//
LOCUS       DATBSZ010000313        11812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1569402, whole genome shotgun sequence.
ACCESSION   DATBSZ010000313 DATBSZ010000000
VERSION     DATBSZ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11812
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569402"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..984)
                     /locus_tag="VIG70_20235"
     CDS             complement(31..984)
                     /locus_tag="VIG70_20235"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8252929.1"
                     /translation="MRWLLAGFIALFSALASAQGFPERPVRIIVPLPPGGSPDTIART
                     LAQGLQGVWPHPVVVENRTGGSQNIGADAVAKSAPDGHTWLLAPDNVFSVNAHIAKQP
                     FDPFADLAPVTLLARIQFLLVVHPDVPAKSVRELIEHAKAKPGELNFGSSGIGSPQFL
                     GGTLLQQMSGTRMNHVPYKGAAPAVADLLAGRIQIWVGAANSLLPHIKADKLRVLGTA
                     AGQRFSALPDTPTIAEAGLPGYALYPWLGMFVPAKTPPEIVARINAEIGRILNSAEVK
                     ARLVPQGMDIATGSPEELARVIREDDARWGKVIRDAGIKPE"
     gene            1078..2610
                     /locus_tag="VIG70_20240"
     CDS             1078..2610
                     /locus_tag="VIG70_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252930.1"
                     /translation="MLTPEELEKLSGAETLFPSPIPVQFVSSDEYMPSPQSEKQKEFE
                     ARIKRSGEALAKKHGMTRRKFFKTAGGMAAAFAAMNEVHAKGSQPLYEVGRNETTDLQ
                     VAQARADGLKNQFVMDMHTHFLREGTPIRAFVAQREAVGKAGWNPTLVGKPQTIDDLM
                     FPNYVKEIFLDSDTKVACISGSYSVDEKFSFLTNQMKFDAREKLNKESGTRRMYSHAI
                     FTPGHDGWLQKVEEENGRLKPDSWKGYTIGDNTNKHLSKWPFRLDDEKVMYPFYERLV
                     KWSKEYPDRPGLKNVCIHKGLFPPSVERQFPHLVGYCTVNDVGQAAKDWPQLNFVIYH
                     SAYRWVAGPGGTAAAAWDQLQRQNRVDWVTDLADIPAQYGVKNVYADLGQIFAHSNMA
                     EPRVAAYMLGYMIKGLGADHVVWGTDSLWTGSPQWQIEALRRLEIPEEIQKKYGLAPM
                     GPADGPVKTAILGGNNARLYGYTPKMMSQLENDRLAYCKSIYERHGGERTNLAYGYIN
                     KG"
     gene            complement(2611..3468)
                     /locus_tag="VIG70_20245"
     CDS             complement(2611..3468)
                     /locus_tag="VIG70_20245"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8252931.1"
                     /translation="MTRTVWLFILALFAAGAVHAQALEGRLKKIVETSTISIAYRADA
                     VPFSFANDKNLPDGFAIDLCKRVVNSIERQLNVLGLKINWVPVTVQTRFEAVAKGQAD
                     MECGSSTMTLTRMKEVDFSSVIFVETTGLLTKATSGVRSISDMSGKKFAVVAGTTNER
                     AIHGQLKRRQLSATVLPFKSRDEALAALEEGKADVFASDKLLLLGAATKAKDPRVLTI
                     LADQLSMEPYGIVLPRGDAALRLAVNTGLAQIYAGEEIVEIFRRWFGALGRPSAILEA
                     AYNLGAIPE"
     gene            complement(3559..4491)
                     /locus_tag="VIG70_20250"
     CDS             complement(3559..4491)
                     /locus_tag="VIG70_20250"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEY8252932.1"
                     /translation="MTILDALILSLPTRNSTVRMRAWRALKDVGCGVLRDGVYVLPAS
                     PTAGAALEKVGSDIRSSGGFAMRVEMHLKTEDQRARLPSLFDRSAEYAALLGQIKDGR
                     ASLRRLGPRKAQTSLERLQRQYDRLDALDFFPGAAKAQATDAISALQRDYREIYGRGE
                     PRVSRRRLRPTDRAKYQARTWATRKNPWVDRLASAWLIKRFIDPAARFVWLERPSSLP
                     RKAIGYDFDGAEFTHVGNRVTFEVLAETFGLDADPALGPIAACVHFLDVGGAPAPDAK
                     GLETVLKGVKEKAGNDDALLAEAMRIFDHFYSAK"
     gene            4598..5551
                     /locus_tag="VIG70_20255"
     CDS             4598..5551
                     /locus_tag="VIG70_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEY8252933.1"
                     /translation="MKYQLKQIYCRPWTLSGLSLPLLESHYENNYGGALRRLNAITAQ
                     LESLDYANAPGYVINGLKREELVALNSTLLHELYFASLGGEGRPTKAMSEALARDFGT
                     VQAWRTQFSAMGYALGGGSGWVLLCWMPRDGRLINQYAAEHSQAVAGGIPILALDMYE
                     HAYHMDFGANAKAYVDAFLRNVDWKAVEGRYEDATKVAPLRPLVQEEFGDLPGVGVEE
                     VQAMLKEGRPLQLIDTRPKHFVSRQQDIATGVTWRDPERVGEWMAELSKETPVVVYCA
                     YGFHVGCRTAIALREAGFDARYMKVGHSGWKAVGGPIEMKV"
     gene            5557..6354
                     /locus_tag="VIG70_20260"
     CDS             5557..6354
                     /locus_tag="VIG70_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEY8252934.1"
                     /translation="MNPAISSSDLRARLDSGAPPIILDVRKAPAFLGASDMISGALRR
                     DPAAVEAWARSLPPASTAVVYCVHGHEVSQNAAKALRAAGIAAHFLEGGIEEGWRASG
                     GPLDSKPKDATTRWVTRERPKIDRIACPWLIARFVDKDAEFLYVPAKQVREVARERHA
                     EPYDVADVHFTHDGDLCSFDAFLNHYRLSDPALKRLALIVRGADTAKLDLAPQAPGLA
                     AISLGLSRIFPDDLEMLKHGMVVYDALYAWCKDGQEEVHTWNPMAYR"
     gene            6449..7054
                     /locus_tag="VIG70_20265"
     CDS             6449..7054
                     /locus_tag="VIG70_20265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252935.1"
                     /translation="MFHRSKLFIVLSVILLSACDGSVRREGPPAPAWLQVVRADTERN
                     RLWVLEPEALSLHDNINGRRLRRIALTEPFFVGEDQACAPDIAWDAAGTVFVSSNVLP
                     VLWRIDPQGFEITRIELKLGADGDKDVGFTALSFAEGGVLIAAGTTYASLWRIDLRTA
                     SASKIASYPLSTPACDPAVLLNARRERGGRMVAVLPAAARQ"
     gene            7056..8012
                     /locus_tag="VIG70_20270"
     CDS             7056..8012
                     /locus_tag="VIG70_20270"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY8252936.1"
                     /translation="METLLLTRSDIAALLDMRDCIDAVETAFRLHAEGKIDPPGILGM
                     HVPGGGFHVKAASLRSYFAAKTNANFPGNPARGLPTIQGAILLFDAADGRLLAIMDSA
                     EVTAVRTGATTGVAAKHLARRSAATLTLLGCGAQAAAQLRAVRAVRELQRVFVSDLDP
                     ARAARFAQEHAAAVAADLPAALAESDICVTCTTSRRFIVENAWVPAGCFVAAVGADNE
                     HKQEIDPALFRKARVVVDSLEQCATIGDLHHALDAGVMTRGEVYAELGELAAGRKPGR
                     QSDSETILFDSTGVALGDVAAAAAVYERALERKRGGAISLAD"
     gene            8025..8927
                     /locus_tag="VIG70_20275"
     CDS             8025..8927
                     /locus_tag="VIG70_20275"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8252937.1"
                     /translation="MELIDFHNHHIPLRFEQDVLRASPASQRPRWEVLARRLSDEELL
                     LRDIREGDINARVVNMSPALMANADGHVPHDTIVAMNDDLAALAARHPGRIHGLASVD
                     AYDGERSAREAERAICDLGLRGLFVDCARGDLMIDAPQARPTLEVAANLGVPILVHPV
                     APQPLTRRMTSYGSVGSLFARGTANSAALIALVEGEVFSELPGLRVVVTSLALGGIAM
                     IAGLSRQSLERVYMDTNICHPVLLRAAIDLLGAENVLAGTDWPINDGPIRSRLTAAMR
                     AARLSDAEQELVAAGNCRRLLRIS"
     gene            8997..9545
                     /locus_tag="VIG70_20280"
     CDS             8997..9545
                     /locus_tag="VIG70_20280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY8252938.1"
                     /translation="MQILGICGSLRKASLNMAALRACNELLPQGMTLRITSIADVPMF
                     NQDVFDAGIPEPAKRLRAEIAEADGVLIASPEYNFSLSAALKNAIDWASRPPSQSWQD
                     KPVAIFSVSQGPMGGARVQYDLRRILGQLWAHVLPRPEVFIGSAPSKFTDGKLTDEAT
                     RKFLTDLLAGFKPWIERMKVRK"
     gene            complement(9508..10245)
                     /locus_tag="VIG70_20285"
     CDS             complement(9508..10245)
                     /locus_tag="VIG70_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8252939.1"
                     /translation="MLRLSDLHASYGAAPVLQGVSLAVEKGETVALLGPNGAGKSTLL
                     GAVTGTIPRRSGTIDLEGLNLAKEASHAIVACGVALVPEGRLVFAPFSVEDNLRLGAV
                     RLQGDSRERFDYVYALFPRLLERKKQPAGTLSGGEQQMLAIGRALMSKPRLLLLDEPF
                     LGLAPLIVSEIRKSLETLRQSGLTLLMVEQKLDIALEFAQRAYVLIKGKVALEDSTAN
                     LARRPDLRDLYFALATTSLPSSAQSTA"
     gene            complement(10229..10984)
                     /locus_tag="VIG70_20290"
     CDS             complement(10229..10984)
                     /locus_tag="VIG70_20290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8252940.1"
                     /translation="MGPILEVSRLTKRFGGLTAVTGLDFELREGEILGLIGPNGAGKT
                     TTFNIIAGAYAADEGEIRFAGESILGLPPHQIARRGIMRTFQHNRPFGGMPVLENVMV
                     GAHTRFTRNPFSQKASVDERHQRQRAEKLIEFVGLGGLHAADVGTLSFGQGRLLEIAR
                     ALAGEPRVILFDEPAAGLTAAELDRLAGIIRGIAERGIAVLLIEHDMRFLLPLASRVV
                     VLNFGARIAEGTPQEVRSQRAVMDAYLGDAAAL"
     gene            complement(10963..>11812)
                     /locus_tag="VIG70_20295"
     CDS             complement(10963..>11812)
                     /locus_tag="VIG70_20295"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8252941.1"
                     /translation="YHNDVYRKLLLWITLALGYNFLFGICGQVAFSHFAFYGIGAYSV
                     VILLFQVGLPLPVAILLGVVICVALALAVAIPATRLEGFYLALVTLALAQLFIVVLNE
                     GGNVTGGAGGIANYRLPPILGWRIAGPAYTVVIVALLAATLAVLWRLDHSWFGRACRA
                     VRDNPEAAAAMGVDVARTKVIVFTLTSALAGIAGMVYAFVDNTVNPPIFGIDNAFLIL
                     FMVIVGGAGRHAGAAIGAILLFLAPFWLSPLVGHHHQLVFGVLMVAVILMQPKGLIGL
                     WDRFSK"
BASE COUNT         2042 a         3918 c         3933 g         1919 t
ORIGIN      
        1 cgggacgtcg cgcatgggcc tatggtgcgc tcattcgggc ttgatgccgg cgtcgcggat
       61 gaccttgccc cagcgcgcgt cgtcctcgcg gatgacgcgg gcgagctctt ccggcgagcc
      121 ggtcgcgata tccatgccct gcggcacgag cctcgccttc acctcggccg aattgaggat
      181 gcggccgatc tcggcgttga tccgggcgac gatctcgggc ggggtcttcg ccggcacgaa
      241 catcccgagc cagggataga gcgcgtagcc gggcaggccg gcttcggcga tggtcggggt
      301 gtccggcagc gcggaaaagc gctgcccggc cgccgtgccc agcacgcgca gcttgtcggc
      361 cttgatgtgc ggcagcagcg agttcgccgc gcccacccag atctgaatgc gtccggcgag
      421 cagatcggcg accgcgggcg ccgcgccctt gtatggcacg tggttcatcc gcgtgcccga
      481 catctgctgc agcagcgtcc cgcccaggaa ctgcgggcta ccgatgcccg aggagccgaa
      541 gttgagctcg cctggcttcg ctttcgcatg ctcgatgagc tcccggaccg acttcgccgg
      601 tacatcggga tgcaccacga gcaggaactg gatgcgcgca aggagcgtga ccggcgcgag
      661 gtcggcgaac ggatcgaacg gctgcttggc gatgtgcgcg ttgaccgaga acacgttgtc
      721 gggcgcgagc agccaggtgt ggccgtcggg cgcgctcttc gccaccgcgt cggcgccgat
      781 gttctggctg ccgccggtgc ggttctcgac taccaccggg tggggccaca cgccttgcaa
      841 cccctgcgcg agggtgcgcg caatggtgtc cggcgaaccg ccaggcggca gcggaacgat
      901 gatccggacc ggccgctcgg ggaacccctg ggcggacgca agtgccgaga aaagcgcgat
      961 aaacccggcc agcagccatc tcatcgtgaa cctcctccgc gcgatttcgc gccgcacagt
     1021 ttacctagtc gggggagcgg aatacgatgg tctttcacag aggaggagca ccccgtcatg
     1081 ttgacccccg aagagctgga gaagctgtcc ggcgccgaaa cgctgttccc gtcgccgatc
     1141 ccggtgcagt tcgtgtcgag cgacgagtac atgcccagcc cccagagcga gaaacagaag
     1201 gaattcgagg cgcgcatcaa gcggagcggc gaagcgctgg cgaagaagca cggcatgacg
     1261 cggcgcaagt tcttcaagac cgccggcggg atggcggcgg cgttcgccgc gatgaacgag
     1321 gtgcacgcaa aaggctctca gccactgtac gaagtgggcc gcaatgaaac tacggatctc
     1381 caggtagccc aggcgcgtgc cgacgggctg aagaaccagt tcgtgatgga catgcacacg
     1441 cacttcctgc gcgaaggcac gccgatccgg gcattcgtcg cgcagcgcga ggcggtcggc
     1501 aaggccggct ggaacccgac gctggtcggc aagccgcaga ccatcgacga cctgatgttc
     1561 ccgaactacg tgaaggagat cttcctcgac agcgacacca aggtcgcgtg catcagcggc
     1621 tcgtactcgg tggacgagaa gttctccttc ctcaccaacc agatgaagtt tgatgcgcgc
     1681 gagaagctca acaaggagtc gggcacgcgc cgcatgtact cgcacgccat cttcacgccc
     1741 ggtcacgacg gctggctgca gaaagtcgag gaggagaacg gccgcctcaa gcccgactcg
     1801 tggaagggct acaccatcgg cgacaacacc aacaagcacc tctcgaagtg gccgttccgg
     1861 ctcgacgacg agaaggtgat gtatccgttc tacgagaggc tggtgaagtg gtcgaaggag
     1921 taccccgacc gccccggcct gaagaacgtc tgcatccaca agggcctgtt cccgccgtcg
     1981 gtcgagcgcc agttcccgca cctggtcggc tactgcaccg tgaacgacgt cgggcaggcg
     2041 gccaaggact ggccgcagct caatttcgtg atctatcact cggcgtatcg ctgggtggcc
     2101 ggacccggcg gtaccgccgc tgcggcatgg gaccagctac agcgccagaa ccgcgtcgac
     2161 tgggtcaccg acctcgccga catcccggcg cagtacggcg tgaagaacgt ctacgccgat
     2221 ctcggccaga tcttcgcgca cagcaacatg gccgagccgc gcgtcgctgc gtacatgctg
     2281 ggctacatga tcaagggcct gggcgccgac cacgttgtct ggggcacgga ttcactgtgg
     2341 accggctcgc cgcagtggca gatcgaggcg ctgcgccgcc tggagattcc cgaggagatc
     2401 cagaagaagt acggcctcgc gccgatgggg ccggccgacg gtccggtgaa gaccgcgatc
     2461 ctcggcggca acaacgcgcg cctctacggc tacacgccga agatgatgag ccagctggag
     2521 aacgacaggc tcgcctactg caagtcgatc tacgaaagac acggcggcga gcgcaccaac
     2581 ctcgcctacg gctacatcaa caaaggatag ctactcgggg atggcgccca ggttgtaggc
     2641 cgcttcgaga atcgcggagg gccggcctag ggcgccgaac cagcgccgga agatctcgac
     2701 gatctcctcg ccggcataga tctgggcgag gcccgtgttg accgcgaggc gcaacgcggc
     2761 gtcgccgcgc ggcagcacga tgccgtaggg ctccatcgag agctggtcgg cgaggatggt
     2821 gagcacgcgc gggtccttcg ccttcgtcgc cgcgcccagc aggagcagct tgtcgctggc
     2881 gaagacgtcg gccttgcctt cctcgagagc tgcgagcgct tcgtcgcgcg acttgaacgg
     2941 cagcacggtt gcgctgagct gccggcgctt gagctgcccg tggatcgcgc gctcgttggt
     3001 ggtgcctgcg acgactgcga acttctttcc cgacatgtcg gaaatcgagc gcacgcccga
     3061 agtggccttg gtcagcaacc cggtcgtctc cacgaagatg acgctcgaga aatcgacctc
     3121 cttcatgcgc gtgagcgtca tggtgctcga gccgcactcc atgtcggcct ggcccttggc
     3181 cacggcttcg aaccgcgtct gcacggtgac cggcacccag ttgatcttca gccccaggac
     3241 gttgagctgg cgctcgatcg agttgaccac gcgcttgcac aggtcgatcg caaagccgtc
     3301 gggcaggttc ttgtcgttgg cgaacgaaaa cggcacggcg tcggcgcggt aggcgatcga
     3361 gatggtgctg gtctcgacga tcttcttcag gcggccttcg agggcctggg cgtgaaccgc
     3421 cccggcagcg aacagggcga ggatgaaaag ccaaacagta cgagtcatgg ttctcccttt
     3481 ggactagcca agtgtatacg gccgacccgg cactcgctcg cctgattgcg ggcaacaagc
     3541 gcttcgccgc cggccttttt attttgcaga gtaaaaatgg tcgaagatgc gcatcgcttc
     3601 ggcaagcaat gcgtcatcgt tgccggcttt ttccttcacg cctttcagga ccgtctccag
     3661 ccctttggca tccggcgcgg gagcgccacc cacgtcgagg aaatggacgc aggcagcgat
     3721 tgggccgagc gcggggtccg cgtccaggcc gaacgtttcc gcgaggacct cgaaagttac
     3781 gcggtttccg acatgggtga attcagcacc gtcgaagtcg tatccgatcg cctttcgcgg
     3841 caggctgctc gggcgctcaa gccacacgaa gcgcgcggcg gggtcgatga accgcttgat
     3901 cagccatgcg cttgccaggc ggtccaccca tggattcttg cgcgtcgccc atgtgcgggc
     3961 ttggtacttc gcgcgatcgg tcgggcggag ccggcggcgc gacaccctcg gctcaccccg
     4021 tccataaatc tcgcggtaat cgcgctgcaa cgccgaaatc gcgtccgtgg cctgggcttt
     4081 cgccgcgccg ggaaaaaagt ccagggcgtc gagcctgtcg tactggcgct gcagccgctc
     4141 gaggctggtt tgcgctttac gcggaccgag tcgacgcagg ctggcgcggc catccttgat
     4201 ttgaccgagc agcgctgcgt actcggcgct gcggtcgaac aggctgggca gacgcgcgcg
     4261 ctggtcctcg gtcttgaggt gcatctcgac cctcatggca aaaccgccgc tggagcggat
     4321 gtcggatccg actttctcga gcgcggcgcc tgccgttgga ctcgcgggca gcacgtacac
     4381 gccatcgcgc agtacgccgc agccgacgtc tttcagagca cgccatgcac gcatccgcac
     4441 ggtcgagttg cgagtcggca ggctgagaat cagcgcatcg aggattgtca ttctgttata
     4501 ggtatcacgg attagtgata aaagataggg accgcctgtg gacgcgacgg cgctcgagtg
     4561 acaggctctg gtcctcgcag actcaaaagg agcacgcgtg aaataccagc tgaagcagat
     4621 ctactgccgt ccctggacgt tgagcggcct ttcactgccg ctcctcgaga gtcattacga
     4681 gaataactac ggcggggcgc tgcgacgcct gaacgccatt accgcgcagc tcgaatcgct
     4741 cgactacgct aacgcgcccg gctatgtgat caacggcctg aagcgcgagg agctggtcgc
     4801 gctcaattcc accctgctgc acgagctcta cttcgccagc ctcggtggcg aaggtagacc
     4861 caccaaggcg atgtccgaag cgctggcgcg cgacttcggc acggtgcagg cctggcgcac
     4921 ccagttttcc gccatgggct atgccctggg cggcggctcg ggctgggtcc tgctctgctg
     4981 gatgccgcgc gacgggcgtc tcatcaatca gtatgcggcc gagcacagcc aggccgtggc
     5041 cggcggcatc ccgatcctcg cgctcgatat gtacgagcac gcgtaccaca tggacttcgg
     5101 cgctaacgcg aaggcctacg ttgacgcatt cctgcgcaac gtcgactgga aggcagtcga
     5161 gggccgctac gaggatgcaa ccaaggtggc gccactccgt cctctggtgc aggaggagtt
     5221 cggcgacctg cccggcgtcg gcgtcgagga agtgcaggcc atgctgaaag agggtaggcc
     5281 gcttcagctc atcgacacgc gcccaaaaca ttttgtttcg aggcagcagg acatcgccac
     5341 gggcgtcacc tggcgcgatc cggagagagt cggcgaatgg atggctgagc tttccaaaga
     5401 gaccccggtc gtcgtgtact gcgcctatgg ctttcacgtc ggctgccgca ctgcgattgc
     5461 gctgagagaa gccggattcg acgcccggta catgaaggtc ggtcactcag gctggaaggc
     5521 cgtcggcggc ccgatcgaga tgaaggtctg aaaaccatga acccagccat ctcgtcgtcc
     5581 gaccttcgcg cgcgcctgga ctccggcgcg ccacccatca tcctcgacgt gcgcaaagcg
     5641 cccgcgtttc tcggcgcatc cgacatgatc tcgggagcgc tgcggcgcga ccccgcggct
     5701 gtcgaggcgt gggccaggtc gctcccgccg gcgagcacgg ccgtcgttta ttgcgtgcat
     5761 ggccacgagg tcagccagaa cgcagccaag gcgctgcggg ccgccgggat tgcggcgcat
     5821 tttctcgaag gcggcatcga ggaaggctgg cgggcgagcg gcggtccgct cgattcgaag
     5881 cccaaggatg caaccacgcg ctgggtaacg cgcgagcggc cgaaaatcga tcggatcgcc
     5941 tgtccctggc tcattgcccg cttcgtcgac aaggatgcgg agttcctcta cgtgccggcg
     6001 aagcaggtgc gcgaggttgc gcgggagcgc cacgcggagc cttacgacgt cgccgacgtc
     6061 catttcacgc acgatggcga cctctgcagc ttcgatgcgt ttctgaatca ctatcgcttg
     6121 agcgacccgg cgctcaagcg cctggccctg attgttcgcg gtgcagatac ggcgaagctt
     6181 gacctggcgc cccaggcgcc ggggctggcc gcaatatcgc tcgggctttc gcgcatcttt
     6241 cccgatgatc tcgagatgct gaaacacggc atggtcgtct acgacgccct gtacgcatgg
     6301 tgcaaggacg gtcaggagga agttcatacc tggaatccca tggcttaccg ctgaagaatt
     6361 accgtcgggc ccgtcgcagt cgcccggcta cccaaggcat atcgcccaag gactgcaccg
     6421 tcccccgctt gcggggagga gaggcgatat gtttcatcga tccaagcttt tcatcgtttt
     6481 gtcggtaatt ctgctttccg cgtgcgacgg cagcgtgcgc cgtgaaggcc cgcctgcgcc
     6541 tgcctggctc caggtggtgc gcgccgatac cgagcgcaat cgcctatggg tgctcgagcc
     6601 cgaggcgctg tcgcttcacg acaacatcaa cggccggcgc ttgcggcgca tcgcgctgac
     6661 ggagccgttc ttcgtcggcg aagaccaggc ctgcgcgccg gacatcgcgt gggacgcggc
     6721 cggcacggtg ttcgtctcca gcaacgtgct gccggttcta tggcgcatcg acccgcaggg
     6781 cttcgagatc acccgcatcg agctcaagct cggcgccgat ggcgacaagg acgtgggctt
     6841 caccgcgctg tcgttcgccg aaggtggcgt tctgatcgcg gcgggtacca cctatgcgtc
     6901 cttgtggcgt atcgatctgc ggaccgcaag cgcgagcaag atcgcgtcct atccgctctc
     6961 cacgcccgcg tgcgacccgg cggtgctgct caacgcgcgc cgggagcgcg gtggacgcat
     7021 ggtcgcggta ttgcctgcgg cggcaaggca gtaacatgga aacgctcctt ctcacgcgtt
     7081 ccgacatcgc ggcgctgctc gacatgcgtg actgcatcga cgcggtggaa acggcttttc
     7141 gcctgcatgc cgagggaaag atcgacccac cgggaattct cgggatgcac gttcccggtg
     7201 gcggattcca cgtcaaggcg gcgagcttgc gctcgtactt cgccgccaag accaatgcca
     7261 actttcccgg aaatccggcg cgcggtttgc ccacgatcca aggcgcaatt ctgttgttcg
     7321 acgcggcgga cggccgcctg ctggcgatca tggattccgc cgaagtcacg gccgtgcgaa
     7381 ccggtgcgac gaccggagtg gcggccaagc atctcgccag gcgatccgcc gccaccctca
     7441 ccctcctcgg ctgcggcgcg caagcggccg cccagttgcg cgcagtgcgt gcggtgcgcg
     7501 agctgcagcg cgtctttgtc tcggatctcg atccggcgcg agccgcgcgc ttcgcccagg
     7561 agcatgccgc agccgtggcg gccgacttgc ccgccgcgct cgccgagagc gacatctgcg
     7621 tcacttgcac gacgtcgcgc cgtttcatcg tcgagaacgc ctgggtgccg gcgggctgct
     7681 tcgtcgcggc ggtcggcgcg gacaacgagc acaagcagga gatcgatccc gccctgttcc
     7741 gcaaggcccg cgtggtcgtc gacagcctcg agcagtgcgc aacgatcggc gacctgcatc
     7801 acgcgctcga tgcgggagtg atgacgcgcg gcgaggtgta cgcagagctc ggtgagctcg
     7861 ccgccggccg caagcccggg cgccagagtg acagcgaaac catcctgttc gattccaccg
     7921 gcgtcgcgct cggcgacgtc gccgccgcgg ccgcggtgta cgagcgcgcg ctcgagcgca
     7981 agcgcggcgg cgcaatctcg ctggccgact gaaaggagca tcccgtggaa ctcatcgact
     8041 ttcataacca ccatattcct ttgcgcttcg agcaggatgt gctgcgggca tcgccggcca
     8101 gccagcgtcc acgctgggag gtcctggcgc gccgactgtc ggacgaggag ctcctgctac
     8161 gcgacattcg cgagggcgac atcaacgcgc gcgtagtcaa catgtccccc gccctgatgg
     8221 cgaacgccga cgggcatgtg ccgcacgaca ccatcgtggc catgaacgac gatcttgcgg
     8281 cgctggccgc gcggcatccg ggccgcatcc acggcctggc ttcggtcgac gcctacgacg
     8341 gcgagcggtc cgcgcgcgag gccgagcgcg cgatctgcga cctcggcctg cggggactct
     8401 tcgtcgactg cgcgcgcggc gatctgatga tcgacgcgcc gcaggcgcgt ccgacacttg
     8461 aggtcgctgc aaacctcggc gtgccgatac tcgtccaccc ggttgcgccg cagccgctga
     8521 cgcggcggat gacgtcctat ggatccgtcg gtagcctgtt cgcgcgcggc acggcgaatt
     8581 ccgctgcgct catcgcgctg gtcgaaggcg aagtgttctc cgaactgccc ggcctgcgcg
     8641 tcgtcgtgac gtcgctcgcc ctcggcggca tagcgatgat cgcggggctc tcgcgccaga
     8701 gcctggagcg ggtgtacatg gacaccaaca tctgccatcc cgtcctgctt cgcgccgcaa
     8761 tcgacctgtt gggcgccgaa aacgtgctcg ccggcaccga ctggccgatc aacgacgggc
     8821 cgatccgcag ccggctgacc gccgcgatgc gggcggcacg cctttccgat gccgagcagg
     8881 agctcgtcgc cgccggcaac tgccggcggc tcctgcgaat ttcctgacgc gatgccggca
     8941 ggcctggctc gaaacgatgg tcgcgcacct gcccgagcgc cgctagactc gcgaccatgc
     9001 aaatcctcgg aatctgcggc agcctgcgaa aggcgtcgct caacatggcg gcgctgcgcg
     9061 cctgcaacga gctcctgccc cagggcatga cgctgcgcat caccagcatc gcggacgtcc
     9121 cgatgttcaa ccaggacgtg ttcgacgccg ggatccccga gccggcgaag cgcctgcgcg
     9181 ccgagatcgc cgaggccgac ggcgtgctga tcgccagccc cgagtacaac ttctcgctca
     9241 gcgcggcgct caagaacgcg atcgactggg cctcgcgccc gccgagccag tcctggcaag
     9301 acaagccggt ggcgatcttc tcggtgtccc agggaccgat gggcggcgcg cgcgttcagt
     9361 acgacctgcg gcgcatcctc ggccagctct gggcgcacgt cctgccgcgg cccgaggtgt
     9421 tcatcggctc ggcgccgagc aagttcaccg atggcaagct cacggacgag gccacccgca
     9481 agttcctcac cgatctcctc gccggcttca agccgtggat tgagcggatg aaggtaagga
     9541 agtagtagcc aacgcgaagt aaaggtctcg caggtccgga cggcgcgcga ggttggcagt
     9601 gctgtcttcc agggcaacct tccccttgat cagcacgtac gcgcgctgcg cgaactccag
     9661 cgcgatgtcc agcttctgct cgaccatcag gagcgtgagg ccgctctgcc tcagcgtctc
     9721 gagcgatttg cggatctcgg aaacgatgag cggcgcgagt cccaggaagg gctcgtcgag
     9781 gagcagcagg cgaggcttgc tcatgagcgc ccggccgatc gccagcatct gttgctcgcc
     9841 gccggacagc gttcccgcgg gctgcttctt tctttcgagc agcctgggaa agagcgcgta
     9901 gacgtaatcg aagcgttcgc gcgagtcgcc ttgcagccgc accgcgccga ggcgcaggtt
     9961 gtcctcgacg ctgaagggcg cgaagacaag cctaccttcc ggcaccagtg ccacgccgca
    10021 ggcgacgatg gcgtggctcg cttccttcgc caggttcagt ccttccaggt cgatcgttcc
    10081 gctgcgccgc gggatcgttc cggtgaccgc gccgagcagc gtcgacttgc cggcgccgtt
    10141 ggggccgagt agcgccacgg tctcgccctt ctccacggcg agcgacacgc cctgcagcac
    10201 cggggcggcg ccgtacgagg cgtgcaggtc agagagccgc agcatcgccc agataggcat
    10261 ccatcacggc gcgctgcgag cgcacttcct gcggcgtgcc ttcggcgatg cgcgcgccga
    10321 agttgagcac caccacgcgg ctcgcgagcg gaagcaagaa tcgcatgtcg tgctcgatca
    10381 ggagcaccgc gatcccgcgc tcggcgatgc cgcggatgat gccggccagg cggtcgagct
    10441 cggccgccgt cagtcccgcg gccggctcgt cgaacaggat gacgcgcggc tcgccggcga
    10501 gcgcgcgcgc gatctcgagc agccgcccct ggccgaagga cagcgtgccg acgtcggcgg
    10561 cgtgcaggcc gccgaggccg acgaactcga tcagcttctc cgcccgctgc cgctgatgtc
    10621 tttcgtcgac agacgctttc tgggagaacg gattccgcgt gaaccgcgta tgcgcgccca
    10681 ccatcacgtt ctccagcacc ggcatgccgc cgaacggacg gttgtgctgg aaggtgcgca
    10741 tgatgccgcg gcgcgcgatc tggtgcggcg gcaggccgag gatcgattcc ccggcgaagc
    10801 ggatctcgcc ttcgtccgcc gcgtaggcgc cggcgatgat gttgaaggtg gtggtcttgc
    10861 cggcgccgtt cggcccgatc aggccgagga tctcgccctc gcgcagctcg aagtcgagcc
    10921 cggtcacggc ggtcaggccg ccgaagcgct tggtgagcct gcttacttcg agaatcggtc
    10981 ccacagcccg atcagtccct tcggttgcat caggatcacg gccaccatca gcacgccgaa
    11041 cacgagctgg tggtggtgcc cgacgagcgg cgacagccag aacggcgcga ggaacagcag
    11101 gatcgcgccg atcgcggcgc ccgcgtggcg gcccgcgccg ccgacgatca ccatgaacag
    11161 gatcaggaac gcgttgtcga tgccgaagat cggcgggttc accgtgttgt cgacgaaggc
    11221 gtagaccatg ccggcgatgc cggcgagcgc gctggtgagc gtgaacacga tcaccttggt
    11281 gcgcgccaca tccacgccca tggccgcggc ggcctcgggg ttgtcgcgta ccgcgcggca
    11341 ggcgcggccg aaccacgaat ggtcgagccg ccacagcaca gccagggtcg cagcgagcaa
    11401 ggccacgatc accaccgtgt acgcggggcc cgcgatgcgc cagccgagga tcggcggcag
    11461 ccggtagttc gcgatgccgc cggcgccgcc ggtgacattg ccgccttcgt tcagcaccac
    11521 gatgaaaagc tgcgccagcg ccagcgtcac cagcgcgaga tagaagccct ccagccgcgt
    11581 cgccgggatc gcgactgcga gcgccagcgc gacgcagatc accacgccga gcagaatcgc
    11641 gaccggcagc ggcaggccga cctggaacag caggatcacc acgctgtagg caccgatgcc
    11701 gtagaaggca aagtgggaga acgccacctg cccgcagatg ccgaacagga agttgtagcc
    11761 gagcgcgagc gtgatccaga gcaggagctt gcgatagacg tcgttgtgat ag
//
LOCUS       DATBSZ010000314        11016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_2047956, whole genome shotgun sequence.
ACCESSION   DATBSZ010000314 DATBSZ010000000
VERSION     DATBSZ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047956"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VIG70_20300"
     CDS             complement(<1..669)
                     /locus_tag="VIG70_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8252942.1"
                     /translation="MVEFKSVSKRYGGVTAVDDISFSIEPGTLATLLGPSGCGKTTTL
                     RLIAGLEMVSSGTITIGGRDVTSAPASERDVSMVFQSYALFPHMNVLENVCYGLRRSG
                     LAKAAADARAREGLAQVGLEGYESRQPSELSGGQQQRVAVARALVLKPKVLLFDEPLS
                     NLDARLRRQMREEIRELQQRLGLTVVYVTHDQQEAMAVSDRIIVMNGGLIEQEGAPRD
                     LYERP"
     gene            complement(669..2723)
                     /locus_tag="VIG70_20305"
     CDS             complement(669..2723)
                     /locus_tag="VIG70_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY8252943.1"
                     /translation="MLLPWYAIEGARWGAPALLHGFQGKPWLLLLFLPVIAALATRPW
                     RLERIRAGTLLMLIGITGLLLLIGQGFLFRHPNITPAGWPTQPGMGYGAMIAGLGFLM
                     LLCLGLAARGFCRGDAFVVSAIGLVVATITAFVFFPILVMLRSAFEGNFLPSFFDRSI
                     WGLDCLYSSLRCGVAWNTVFLAIVVGAGSTVLGLAFALIAARTDFRFKGVLRVMSVLP
                     IITPPFVIGLAVILLFGRAGAASTLLFDWFDIPRSRWIYGLPGVALAQLLAFTPIAFL
                     VLLGVVQGISPSLEEASQTLRARGWTTFRTVTLPLLRPGLAAAFLLGFVESMADFGNP
                     LVLSGNFEVLSVKIFFAVVGASHDQSRAAVLALVLLVFTLAAFWAQQRWLGRASYTTV
                     TGKGDAGLPVPLPRGLRWLCYGIALPWSALTLLVYVTIFVGGFVKSVGRDYSFTLAHF
                     LAGFGVEATERGLHFYGAAWPSLTTTLIIATVAAPLTAGVGLLTAWLLSRQNFAGKRA
                     FEFGTLLSFAIPGTVVGISYILAFNVPPIEITGTAAILIICFVFRNMPVGVRAGIATL
                     SQIDKSLDEASHTLGARSATTIRRVVVPLLRPAIIASLVYSFVRAMTAVSAVIFLVTA
                     EYNMATAYIVGRVEAGEFGIAIAYSCVLIAIMLAVIGLIQVGVGERKLGRRDTVALQG
                     AA"
     gene            complement(2753..3763)
                     /locus_tag="VIG70_20310"
     CDS             complement(2753..3763)
                     /locus_tag="VIG70_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8252944.1"
                     /translation="MKHLFAALALLVPTLAAAQGNLVLYCTPQEEWCRGMVTAFERKT
                     GIKVAMTRKSSGENYAQVKAEASNPRGDIWWGGTGDPHLQAAEEGLLDEYRSPLLKDL
                     APWAVKQAEQSKYRTVGVYAGVLGFGYNTELLAKKKLPEPKCWGDLASDKYRDEVQVA
                     DPNSSGTSYTMLATIVQLHGEDKGFDLMKRMHKNINQYTRSGAAPAKAAASGETLIGI
                     TFLHDLVTFAVQGAPLKLVAPCEGTGYEVGSMSIIKGGKNPKQARIWYDWALTPEAQA
                     IGAETKAYQIPSNKNAKVPPQAPRLDQTKVIDYDFAKYGSSAERKRLLSKWDKEVKAL
                     PK"
     gene            complement(3760..4575)
                     /locus_tag="VIG70_20315"
     CDS             complement(3760..4575)
                     /locus_tag="VIG70_20315"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY8252945.1"
                     /translation="MTTQELDAREETLIAVAREAGELARELFSKPIEIKLKGKQDYIT
                     EADGQVERMIISRLKDRFPQDAFLGEEGGASHSGKSDALWVIDPIDGTANFAHGIPHF
                     CVSIAFMAGNELALGAIAAPMYGELYRARLGRGAFLNDRAMRVTDADIKQAVIELGWS
                     SRRPTRAYTSLVERVLATGATFLRAGSGALGLAYVAAGRTHGYCELHINSWDTLAGLL
                     MVREAGGWTNDFLAGHGLERGNAVLACAPGLKDALVAATGVGQANEKFERRMS"
     gene            complement(4572..5456)
                     /gene="iolE"
                     /locus_tag="VIG70_20320"
     CDS             complement(4572..5456)
                     /gene="iolE"
                     /locus_tag="VIG70_20320"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HEY8252946.1"
                     /translation="MVRIGINPLTWTNDDLPELGESNSLETCLTEAKLAGYEGVELGR
                     KFPRDPAVLGPILRKHQLNLVSGWYSARLLERSVEDEIKALQPHATLLKALGAREMVF
                     CEVSRCVHGDRKIPLSKRPQLAEKEWQQFSERLDAVARELKNQGLKMAYHHHMGTVIQ
                     AEDEVDRMMDSTKAVGLLLDTGHLTFAGGDPLRALKRHAKRIVHVHCKDIRKDKLAQA
                     RQKDMSFLDAVLGDVFAVPGDGSIDFEPVLKGLAGAGYDGWLVVEADQDPAKAHPLTH
                     ARIGHDCLRPLARRYWKE"
     gene            complement(5457..7274)
                     /gene="iolD"
                     /locus_tag="VIG70_20325"
     CDS             complement(5457..7274)
                     /gene="iolD"
                     /locus_tag="VIG70_20325"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEY8252947.1"
                     /translation="MATVRITTAEAVVRFLAAQQIEGEIPLFAGVWAIFGHGNVAGLG
                     PALHAARRKLPTLRAHNEQAMAHAAIAFAKAHARRRMMACTSSIGPGASNMVTAAAVA
                     HVNRLPVLFLPGDVFASRRPDPVLQQLEAFGDPTASVNDCFRPVSRYFDRIMRPEQIL
                     VSLPAALRVLLDPAECGPATLALPQDVQTEVYDYPAEFFRRRVHRVRRPLPDGDELAA
                     AVQLFKKARKPLIVAGGGVKYALAEDRLAAFAEAHGIPVAETQAGKGTLAWDHPCNAG
                     AIGVTGTSAANTLAARADLVLAIGARLGDFATGSRALFAEAKLVQLNVASFDAAKHGA
                     LALVADARAALDGLSRDLKGWKAPAAWTGEATRLTRAWNRKVDEIVAPSRGKPTDAQV
                     LGIVNQNLPGNGIVVCAAGGLPGELHKLWRATDSTSYHLEYGYSCMGYEIAGGLGVKL
                     AHPKREVCVLVGDGSYLMLNSEIATSVALGAKLVIVVLDNRGFGCIERLQAATGGASF
                     NNMLGDAAPRVDFAAHARSLGAQAEKVDGVTALSAALKKALRAKRTSVIVIDTDARRG
                     TAEGGAWWDVAVTEAPRSAGQKQARGAYERARKKQALGA"
     gene            7310..8305
                     /gene="iolG"
                     /locus_tag="VIG70_20330"
     CDS             7310..8305
                     /gene="iolG"
                     /locus_tag="VIG70_20330"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEY8252948.1"
                     /translation="MTEIAQFGAGRIGQIHASNIAAMKDVRLRYVVDVNEKAAQALAA
                     RYGARVVAEREAFADRAVDAVLIASSTDTHARLAIEAARAGKAIFCEKPIDLSLSKVD
                     SCLEEVRKAGVPMFVGFNRRFDPSFAALKRRLDAGEIGAVEQVVISSRDPAPPPPAYV
                     KVSGGMFRDMTIHDFDMARWLLGEEPVEVYAMGECLVDRKIGAAGDIDSAMVLLRTAT
                     DKMCHINNSRRAAYGYDQRIEVAGAKGMLRAENVGETTVEHFGARGTVSDRPLDFFLE
                     RYADAYRAEMAAFLRALKEKAPMPVGARDGRQALVLAEAALESHKKKKPVKVGNG"
     gene            8298..9290
                     /gene="iolG"
                     /locus_tag="VIG70_20335"
     CDS             8298..9290
                     /gene="iolG"
                     /locus_tag="VIG70_20335"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEY8252949.1"
                     /translation="MVNFALFGAGRIGRMHAENLVRRSDAKLLYVVDSKPEAARALAE
                     QLGAKAADADRAFSDPQVNAVLIASSTGSHADLSIAAARAGKAIFCEKPVDLTLAKVE
                     QCIAAVKKAKVPMFVGFNRRFDPSFRELKARLDAGAVGALEQVIITNRDPGLPDLRFL
                     ATSGGLYLDFTIHDFDMARWLLGEDPVEVFAWGAVLVDPRVRTEGKDIDTAMLLLRTK
                     SGKMCHINNTRRAVYGYDQRIEVFGSKGMLRAGNLAPTSVERSGEQATCGDNPWPNFQ
                     TRYAAAYAAELSSFIQSVEKRKPAEIPPEDSRKVLLLCEAALKSSQTGKPVKVG"
     gene            9332..10384
                     /locus_tag="VIG70_20340"
     CDS             9332..10384
                     /locus_tag="VIG70_20340"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8252950.1"
                     /translation="MDQAERKNTAQHALVYVAVAVGVVVVLVLLWYAIDVVLLAFIGI
                     LLAILLRAPADWLSERAGLPERWSLVLVGSVFLALLVLGATLFGRGIAAQALALVDRI
                     PEIVETLKSELAETEIGSRAVALAESSGMFSGGGGQFIGRGLGLIGSTFGAIANVLIV
                     LFFAVFMAAQPRPYVEGLLFLVPRRRRNRVREVLHEIGHVLRRWLVGQTVLAACVATL
                     TGVGLVLLGAPFPFALALLAGLMEFVPYIGPFIAAIPAILVGFAEGPQLALSIGLLFV
                     AIQLTESYLLAPLVQHRAVHLAPAAVLFAQVLMGAIVGALGVAVATPLAAAAMVAVSM
                     LYVEDALGDKSAKPRS"
     gene            complement(10381..10650)
                     /locus_tag="VIG70_20345"
     CDS             complement(10381..10650)
                     /locus_tag="VIG70_20345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252951.1"
                     /translation="MMLRAALALLLFVLGGCTLFPLSEADCKPASWRERGYADGFAGH
                     PPQDLRLIPECRERFGVEIGREDYLAGWKHGYDEWDRQMGSMGVD"
     gene            complement(10647..10901)
                     /locus_tag="VIG70_20350"
     CDS             complement(10647..10901)
                     /locus_tag="VIG70_20350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252952.1"
                     /translation="MFLLALLGGCALFPLGEADCKPASWTSRGYADGFGGHLPQDMRL
                     MPECRERYGVEVGQAEYLAGWKDGHDEWDRLMGSMRKHSQ"
BASE COUNT         1776 a         3801 c         3685 g         1754 t
ORIGIN      
        1 cggacgctcg tagaggtcgc gcggcgcgcc ctcctgctcg atcaggccgc cattcatcac
       61 gatgatgcgg tccgacaccg ccatcgcctc ctgctggtcg tgggtgacgt agacgacggt
      121 gaggccgagc cgctgctgca actcgcggat ttcctcgcgc atctggcggc gtaggcgggc
      181 gtcgagattg ctgagcggct cgtcgaacaa cagcaccttg ggcttcagca ccagggcgcg
      241 cgccaccgcc acgcgctgct gctggccgcc ggacagttcc gacggctggc ggctttcgta
      301 cccctcgagc ccgacctgcg cgaggccctc gcgcgcccgc gcatcggccg cggccttggc
      361 cagccccgag cgccggaggc catagcagac attctccagc acgttcatgt ggggaaacag
      421 cgcgtaggac tggaacacca tgctgacgtc gcgctcggac gcgggcgccg aggtcacgtc
      481 gcggccgccg atggtgatgg tccccgagct caccatctcg aggcccgcga tcagccgcag
      541 cgtggtggtc ttgccgcagc ccgagggccc gagcagggtg gcgagcgttc cgggctcgat
      601 actgaacgag atgtcgtcca ccgcggtcac gccgccgtag cgcttgctta cgctcttgaa
      661 ctcaaccatc aggccgcccc ctgcaacgct acggtatcgc gccgcccgag cttgcgctcg
      721 ccgacgccca cctggatcag gccgatcacc gccagcatga tggcgatcag cacgcacgag
      781 taggcgatgg cgatgccgaa ctcgccggct tccacgcgcc cgacgatgta ggcggtcgcc
      841 atgttgtatt cggcggtgac gaggaagatc accgcgctga ccgcggtcat ggcgcgcacg
      901 aagctgtaga ccagcgacgc gatgatcgcg gggcgcagca acggcaccac cacgcggcgg
      961 atcgtggtcg cggaacgcgc gcccagcgtg tgcgaggctt catccagcga cttgtcgatc
     1021 tgcgacagcg tggcgatgcc ggcgcgcacg ccgaccggca tgttgcggaa cacgaagcag
     1081 atgatgagga tcgccgcagt tccggtgatt tcgatcggcg gcacgttgaa ggcgaggata
     1141 tagctgatgc cgaccaccgt gccggggatg gcgaaggaaa gcagcgtgcc gaactcgaag
     1201 gcgcgcttgc cggcgaagtt ctggcgcgac agcagccacg cggtgagcag gccgaccccg
     1261 gcggtgagcg gcgcggcgac ggtggcgata atcagcgtgg tggtgagcga gggccacgcg
     1321 gcgccgtaga agtgcagccc gcgctccgtc gcctcgacgc cgaagccggc gaggaaatgc
     1381 gccagcgtga agctgtagtc gcggccgacc gacttgacga agccgccgac gaagatggtg
     1441 acgtagacga ggagcgtgag cgccgaccat ggcagcgcga tgccgtagca cagccagcgc
     1501 aggccgcgcg gcagcggcac cggcagcccg gcgtcgccct tgccggtgac cgtggtgtag
     1561 gaggcgcgcc cgagccagcg ctgctgcgcc cagaacgcgg ccagcgtgaa caccagcagc
     1621 acgagcgcca gcaccgcggc gcggctctgg tcgtgcgagg cgccgaccac ggcaaagaaa
     1681 atcttcaccg acaggacctc gaaatttccg gacagcacca gcgggttgcc gaagtccgcc
     1741 atgctctcga cgaaaccgag caggaaggcg gcggcgagcc ccggccgcag cagcggcagc
     1801 gtcaccgtgc ggaaggtcgt ccagccgcgc gctcgcagcg tctgcgaggc ttcctcgagc
     1861 gaagggctga tgccctgcac cacgccgagc agcacgagaa aggcgatcgg cgtgaacgcg
     1921 agcagctgcg cgagcgccac gcccggcaga ccgtagatcc agcgcgagcg cgggatgtcg
     1981 aaccagtcga agagcaacgt gctcgccgcg ccggcgcggc cgaagagcag gatcaccgcc
     2041 aggccgatca cgaacggcgg agtgatgatg ggcagcacgc tcatcacgcg cagcacgccc
     2101 ttgaagcgga aatcggtgcg cgccgcgatc agggcgaagg cgagcccgag cacggtggag
     2161 ccggcgccga ccacgatcgc gaggaacacc gtgttccagg ccacgccgca gcgcaggctc
     2221 gaatacaggc agtcgaggcc ccagatcgag cggtcgaaga agctggggag gaagttgccc
     2281 tcgaacgcgc tcctcagcat cacgaggatc ggaaagaaca cgaacgcggt gatcgtcgcc
     2341 accacgaggc cgatcgcgct gacgacgaag gcgtcgccgc ggcagaagcc gcgcgccgcg
     2401 agcccgaggc agagcagcat caggaagccg agccccgcga tcatcgcgcc gtatcccatg
     2461 cccggctggg tcggccagcc ggcgggcgtg atgttcggat ggcggaacag gaagccctgc
     2521 ccgatcagca acaggagccc ggttattcca atcaacatca aaagggtacc ggcgcgtatg
     2581 cgctccaggc gccagggacg cgtcgccagc gccgcaatca ccggcagaaa cagcaggagc
     2641 agccagggtt taccctgaaa gccgtgcaac agcgcgggcg cgccccagcg cgcgccctcg
     2701 atcgcgtacc agggcagcag caccagtgct gccgccgtgg cggcgatcca gattacttgg
     2761 gtagtgcttt gacttccttg tcccacttcg acagcagccg cttgcgctcc gccgacgagc
     2821 cgtacttcgc gaagtcgtag tcgatgacct tggtctggtc gagccgcggc gcctgcggcg
     2881 gcaccttggc gttcttgttg gacgggatct ggtaggcctt ggtctcggcg ccgatcgcct
     2941 gcgcctcggg agtgagcgcc cagtcgtacc agatcctcgc ctgtttcggg ttcttgccgc
     3001 ccttgatgat gctcatggac cccacctcgt aaccggtgcc ttcacacggg gcgaccagct
     3061 tgagcggcgc gccttgcacc gcgaaggtca ccagatcgtg caggaacgtg atgccgatca
     3121 gcgtctcgcc gctggccgcg gccttggcgg gcgcggcccc cgagcgcgtg tactggttga
     3181 tgttcttgtg catgcgcttc atcaggtcga agcccttgtc ctcgccgtgc agctgcacga
     3241 tcgtcgcgag catcgtgtag gaagttcccg aggagttcgg gtcggcgacc tgaacctcgt
     3301 cgcggtactt gtcggaggcg aggtcgcccc agcacttcgg ctcgggcagc ttcttcttcg
     3361 ccagcagctc ggtgttgtag ccgaacccca ggacgccggc atagacgccg accgtgcggt
     3421 acttcgactg ttcggcttgc ttcaccgccc agggggcgag gtccttcagc agcggcgagc
     3481 ggtactcgtc cagcaggccc tcttccgccg cctgcaagtg cggatcaccc gtgccgcccc
     3541 accagatgtc gccgcgcggg ttggaagcct cggccttcac ctgggcgtag ttctcgccgc
     3601 tcgacttgcg ggtcatcgcg accttgatgc cggtctttct ttcgaacgcg gtcaccatcc
     3661 cccggcacca ttcttcctgc ggcgtgcagt acaggaccag gttgccttgg gctgccgcca
     3721 gcgtgggaac cagcagcgcc agtgctgcga acagatgctt catgacatcc tcctctcgaa
     3781 tttttcgtta gcttgcccaa ctccggtcgc ggcgaccaga gcgtccttca gtcccggcgc
     3841 gcacgccagc accgcgttgc cgcgctcgag gccatggccg gcgagaaaat cattggtcca
     3901 cccgccggcc tcgcgcacca tcagcaggcc cgcgagcgta tcccaggaat tgatgtgcag
     3961 ctcgcaatag ccatgcgtgc gcccggcggc cacgtaggcg agcccgagcg cgcccgagcc
     4021 cgcgcgcagg aacgtcgcgc cggtggcgag cacgcgctcg accagcgacg tataggcgcg
     4081 cgtcggcctg cgcgaggacc agccgagctc gatcacggcc tgcttgatgt cggcgtccgt
     4141 gacgcgcatg gcgcggtcgt tgaggaaagc gccccggccc aggcgcgcgc ggtagagctc
     4201 gccgtacatc ggcgccgcga tcgcgccgag cgcgagctcg ttgccggcca tgaacgcgat
     4261 cgatacgcag aagtgcggaa tgccgtgagc gaaattcgcc gtgccgtcga tcgggtcgat
     4321 gacccagagc gcgtcgctct ttccggaatg ggaggcaccg ccttcctcgc cgagaaacgc
     4381 gtcctgcggg aagcggtctt tcaggcgcga gatgatcatg cgctcgacct gcccgtcggc
     4441 ctcggtgatg tagtcctgct tgcccttgag cttgatctcg atcggcttcg agaacagctc
     4501 gcgcgccagc tctcccgcct cgcgcgcgac cgcgatcagc gtctcctccc tggcgtcgag
     4561 ttcctgcgtg gtcactcttt ccagtatcgc cgcgcgagag gcctaaggca atcgtggccg
     4621 atgcgcgcgt gggtcagcgg gtgcgccttc gccgggtcct ggtccgcctc gaccacgagc
     4681 cacccgtcgt agccggcacc ggccaatcct ttcaatacgg gctcgaaatc gatcgacccg
     4741 tcgccgggca ccgcgaacac gtcgccgagc accgcgtcga ggaagctcat gtccttttgc
     4801 ctcgcctgcg cgagcttgtc cttgcggatg tccttgcagt gcacgtgcac gatgcgcttc
     4861 gcatggcgct tgagcgcgcg cagcgggtcg ccgcccgcga acgtgaggtg gccggtatcg
     4921 agaagcagtc cgacggcctt ggtcgaatcc atcatgcggt caacctcgtc ctcggcctgg
     4981 atcaccgtcc ccatgtgatg gtggtaggcc attttcaggc cctggttctt cagctcacgc
     5041 gccaccgcgt cgagccgctc gctgaactgc tgccactcct tttcggcgag ctgcgggcgc
     5101 ttcgacagcg gaatcttgcg atcgccgtgc acgcagcgcg acacctcgca gaagaccatt
     5161 tcccttgcgc cgagcgcctt gaggagcgtc gcatgcggct gcaacgcctt gatctcgtcc
     5221 tcgaccgagc gctcgagcag ccgggcgctg taccagcccg ataccaaatt gagttgatgc
     5281 ttcctgagaa tcggaccgag taccgcgggg tcacggggaa acttcctgcc caactcgacc
     5341 ccttcgtagc cggcgagctt cgcttccgtc aggcaggtct cgagcgaatt gctctcgccg
     5401 agctcgggca ggtcgtcgtt cgtccacgta agcggattga tgccgatgcg gaccatctag
     5461 gctcccaatg cctgcttttt ccgggcgcgc tcgtacgctc cgcgggcctg cttctgcccg
     5521 gccgagcggg gcgcttcggt gacggcgacg tcccaccagg ccccgccttc ggcggtgcca
     5581 cggcgcgcat cggtgtcgat cacgatcacc gaggtccgct tcgcgcgcag ggctttcttc
     5641 agcgcggcgc tcagtgccgt cacgccgtcg accttctcgg cctgcgcgcc gaggctgcgc
     5701 gcatgagcgg cgaaatcgac gcgcggcgcg gcgtcgccga gcatgttgtt gaacgaggcg
     5761 ccgccggtcg ccgcctgcag gcgctcgatg cagccgaagc cgcggttgtc cagcaccacg
     5821 atcaccagct tcgcgccgag cgcgaccgag gtggcgatct ccgagttgag catgaggtag
     5881 ctgccgtcgc ccaccagcac gcacacctcg cgcttcggat gcgccagctt cacgcctaga
     5941 ccgccggcga tctcgtagcc catgcaggag tagccgtatt cgagatggta gctggtcgaa
     6001 tcggtcgcgc gccacagctt gtggagctcg cccgggagcc cgccggcagc gcagacgacg
     6061 attccattcc cgggaagatt ctgattgacg atgccgagca cctgcgcatc ggtgggcttg
     6121 ccccgggagg gagccacgat ctcgtcgacc ttgcggttcc acgcccgcgt gagccgggtc
     6181 gcttcgcccg tccacgccgc cggcgccttc cagcccttca agtcgcggct caagccatca
     6241 agcgccgcgc gcgcgtctgc gacgagggcc agcgcgccgt gtttcgcggc gtcgaacgag
     6301 gcaacgttga gctgcacgag cttcgcttcg gcgaagagcg cgcgcgagcc ggtggcgaag
     6361 tcgcccagtc gcgccccgat ggcaagaacc aggtccgcgc gcgcggcgag cgtattggcg
     6421 gcgctcgtgc ccgtgacgcc gatcgcgccc gcgttgcagg gatggtccca ggcgagggtg
     6481 cccttgcccg cctgagtctc ggccaccggg atgccgtgtg cctcggcgaa cgcggccagc
     6541 cggtcttcgg cgagcgcgta cttcacgccg ccaccggcga cgatcagcgg ctttctcgcc
     6601 ttcttgaaaa gctggaccgc cgcggcgagc tcgtcgccgt caggcaaggg gcggcgcacc
     6661 cggtgcacgc ggcggcggaa gaactccgcc gggtagtcgt aaacctcggt ctgcacgtcc
     6721 tgcggcagcg cgagcgttgc cgggccgcac tcggcgggat cgagcagcac gcgcagcgct
     6781 gccggcaggc tgacgaggat ctgctccggg cgcatgatgc gatcgaaata gcgcgagact
     6841 ggccggaagc agtcgttgac cgacgcggtc ggatcgccga acgcctcgag ctgctgcagc
     6901 accggatcgg gacggcggct cgcgaacacg tcgccgggga ggaagagcac cggcaggcgg
     6961 ttgacgtgcg ccaccgcggc cgcggtcacc atgttgcttg cccccgggcc gatcgagctc
     7021 gtgcaggcca tcatgcgccg gcgcgcgtgc gccttcgcga acgctatcgc ggcgtgcgcc
     7081 atggcttgct cgttgtgagc gcgaagcgtc ggcagcttgc gccgcgcggc gtgcagcgcc
     7141 gggccgaggc cggcgacgtt gccgtggccg aagatcgccc acactccggc aaagagggga
     7201 atctcgcctt cgatctgctg tgcggccagg aagcggacca ccgcttcggc cgtcgtgatg
     7261 cgaaccgttg ccacacacct cccctcgagc aatctattat tacaagaaaa tgaccgagat
     7321 cgcccagttc ggtgccggcc gcatcggcca gatccacgct tccaacatcg ccgcgatgaa
     7381 ggacgtgcgg ttgcgctacg tggtcgacgt gaacgagaag gccgcgcagg cgctcgccgc
     7441 acgctacggc gcaagagtcg tggccgagcg cgaggccttc gccgatcgcg cggtcgatgc
     7501 cgtgctcatc gcctcgtcca ctgacaccca tgcgcgcctc gccatcgagg ccgcgcgcgc
     7561 cggcaaggcg atcttctgcg agaagccgat cgatctgtcg ctttccaaag tggattcctg
     7621 tctggaagaa gtccggaaag ccggggtccc gatgttcgtc ggcttcaatc ggcgcttcga
     7681 cccgagcttc gccgcgctca agcgccgcct cgacgccggg gagatcggcg cggtcgagca
     7741 ggtggtgatc tccagccgcg atccggcgcc gccgccgccc gcctacgtga aggtttccgg
     7801 cggcatgttc cgcgacatga cgatccacga cttcgacatg gcgcgctggc tgcttggcga
     7861 ggagccggtc gaggtgtacg ccatgggcga gtgcctggtg gaccgcaaga tcggtgccgc
     7921 cggcgacatc gactccgcga tggtgctgct gcgcactgcc accgacaaga tgtgccacat
     7981 caacaactcg cgccgcgccg cctacggcta cgaccagcgc atcgaagtcg ccggcgccaa
     8041 ggggatgctg cgcgccgaga acgtcggcga gaccacggtc gagcacttcg gcgcgcgcgg
     8101 cacggtgagc gaccggccgc tcgacttctt cctcgaacgc tacgccgacg cctatcgcgc
     8161 cgagatggct gctttcctgc gtgcgctaaa ggaaaaagcg ccgatgccgg tcggcgcgcg
     8221 cgacggccgc caggcgctgg tgctcgccga agctgctttg gaatctcata aaaagaaaaa
     8281 acccgtcaaa gtcggcaatg gttaacttcg ccctcttcgg cgcgggaaga atcgggcgca
     8341 tgcacgccga gaacctcgtg cggcgcagcg atgcgaagct cctctacgtg gtcgactcga
     8401 agcccgaggc ggcgcgtgcg ctcgccgagc aactcggagc gaaagcggcg gacgcggaca
     8461 gggctttctc ggacccgcaa gtcaacgccg tgctgatcgc gtcgtcgacc ggcagccacg
     8521 ccgacctctc gatcgccgcg gcgcgcgccg gcaaggcgat cttctgcgag aagccggtgg
     8581 atctcacgct cgcgaaagtg gagcaatgca tcgcagcggt gaagaaggcg aaggtgccga
     8641 tgttcgtcgg cttcaaccgg cgcttcgacc cgagcttccg cgagttgaag gcgcgcctgg
     8701 acgccggcgc ggtcggcgcc ctcgagcagg tgatcatcac caaccgcgat ccggggctgc
     8761 ccgacctgcg cttcctcgcc acctcgggcg ggctctatct cgacttcacg atccacgact
     8821 tcgacatggc gcgctggctg ctcggcgagg acccggtcga ggtatttgcg tggggcgcgg
     8881 tgctggtcga tccgcgcgtg cgcaccgagg gcaaggacat cgacaccgcg atgctgctgc
     8941 tgcggacgaa atccgggaag atgtgccaca tcaacaacac gcgccgcgcg gtgtacggct
     9001 acgaccagcg catcgaggtc ttcggctcga agggcatgct gcgcgccggg aatctcgcgc
     9061 cgaccagcgt cgagcgctcg ggcgagcagg cgacctgcgg cgacaatccg tggccgaatt
     9121 tccagacccg ctacgccgcc gcctacgctg cggagctctc gtcgttcatc cagagcgtgg
     9181 agaagcgcaa gcccgccgag attccgcccg aggacagccg caaggtattg ctcctgtgcg
     9241 aagcggcgct caagtccagc cagaccggca agcccgtaaa agtaggatga gaatcaagaa
     9301 ctagcgcgtc gcgggcgggt ccatagaagc catggaccag gccgaacgca agaacaccgc
     9361 gcagcacgcg ctggtttacg tcgccgttgc cgtcggggtc gtcgtcgttc ttgtgctcct
     9421 ctggtatgcg atcgacgtag tccttttggc cttcatcggc atcctgctcg ccatcctgct
     9481 gcgcgcgccg gccgactggc tgagcgagcg cgccgggctc ccggagcgct ggtcgctcgt
     9541 cctggtcggg tcggtgttcc tcgccctgct ggtgctcggc gcaacgctct tcgggcgcgg
     9601 catcgcggcg caagcgctcg cgctcgtcga tcgcatcccg gaaatcgtcg agacgctcaa
     9661 gagcgagctc gccgaaaccg agatcggcag ccgcgccgtg gcgctcgccg agtcgagcgg
     9721 gatgttctcc gggggcggcg gccagttcat cggccgcggc ctcggcctca tcggctcgac
     9781 cttcggcgcg atcgcgaacg tgctgatcgt gctgttcttc gccgtgttca tggccgcgca
     9841 gccgcggccc tacgtcgagg ggttgctctt cctcgtgccg cgcaggcgcc gcaaccgcgt
     9901 ccgcgaggtg ctccacgaga tcgggcacgt gctgcgccgc tggctcgtcg ggcagacggt
     9961 gctcgcggcc tgcgtggcga ctctcaccgg cgtgggcctc gtgctgctcg gcgcgccgtt
    10021 cccgttcgcg cttgcgctgc tcgccgggct gatggagttc gtgccttaca tcgggccttt
    10081 catcgccgcg attccggcga tcctggtggg cttcgccgaa ggaccgcagc tcgcgctctc
    10141 gatcggcctg ctcttcgtcg ccatccagct caccgagagc tatctgctcg ccccgctcgt
    10201 ccagcaccgc gcggtgcacc tcgccccggc ggcggtcctg ttcgcccagg tgctgatggg
    10261 cgcgatcgtc ggcgcgctcg gcgtcgcggt cgccaccccg ctcgcagccg cggcgatggt
    10321 ggcggtgagc atgctctacg tcgaggacgc gctcggcgac aagtcggcca agccgcgctc
    10381 ctagtcgacc cccatcgaac ccatctgccg gtcccactcg tcgtagccgt gcttccatcc
    10441 cgcgaggtag tcctcccgcc cgatttccac gccgaagcgc tcgcggcatt cggggatgag
    10501 acgcaggtcc tggggcgggt gcccggcgaa cccgtcggca tagccgcgtt cgcgccagct
    10561 cgcaggcttg caatccgcct cgctcagcgg gaagagcgtg cagccgccca gcacgaacaa
    10621 cagcaaggcc aacgcggcgc gaagcatcat tgcgagtgct tccgcatgct gcccatcagg
    10681 cgatcccact cgtcatggcc gtctttccat cccgcgaggt attccgcctg cccgacctcc
    10741 acgccgtaac gttcgcggca ttccggcatg agacgcatgt cctggggcag atggccgccg
    10801 aaaccgtcgg catagccgcg gctggtccaa ctcgcgggct tgcaatccgc ctcgcccagc
    10861 gggaaaagcg cgcaacctcc gagcagcgcg aggaggaaca ttacggatct cgacatacgg
    10921 ggcaaacacc gctatcccgg cttgattccg accatgcgct cgacgtagcg cgcgatgagg
    10981 tcgacctcga ggttcacctt tgccccgggc tgcagg
//
LOCUS       DATBSZ010000315        11816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1638276, whole genome shotgun sequence.
ACCESSION   DATBSZ010000315 DATBSZ010000000
VERSION     DATBSZ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11816
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638276"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VIG70_20355"
     CDS             <1..217
                     /locus_tag="VIG70_20355"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY8252953.1"
                     /translation="AAAAPAKTPKDVVTRLHGAMVKALRSPDIAERMNQIGFDVVASS
                     PEEFGAFMKAEVERWTRVVSKGGIKPD"
     gene            223..1290
                     /locus_tag="VIG70_20360"
     CDS             223..1290
                     /locus_tag="VIG70_20360"
                     /inference="COORDINATES: protein motif:HMM:NF007504.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HEY8252954.1"
                     /translation="MVFRKENLTRAIEAIALAGGSEPAEAKLVAENLVTANLMGHDSH
                     GIGMMPRYVEALLEGGLHPNRQVEVKFDAGALLTLDGCAGYGQSVGVQATQMAIARAR
                     EHGTAIVALGNSHHLGRIGHWAEMAVAQGLVSLHFVNVQSFARVAPFAGADRRFGTNP
                     VCIGIPLPGEPPFLLDMATSAVAQGKLRVAHNKRAKIPPGWLIDDQGNPTDDPRWGVV
                     APLGAMACFGEHKGYGLAVACELLGGALTGGGVTDYDNKTQRRVLNGMISIVIDPARL
                     GTQQAFAKDAKSFLVWLRASRAAPGHDRVRIAGEPEREYRAKRERDGVPVDEETWKEI
                     RAAAAKLKLSPERIDALARGG"
     gene            1293..2249
                     /locus_tag="VIG70_20365"
     CDS             1293..2249
                     /locus_tag="VIG70_20365"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY8252955.1"
                     /translation="MRRSFLAIVLLVVSAALGAQEYPVRTVRIVVPFAPGGGTDLIAR
                     VLSQHLSQRLGQSFVVENRPAGSGIVGADHVAKSAPDGYTLLFAFSSLSSSAKLFTKL
                     PYDPVRDFAPVALATTSPLLVVVPAALPAKDLREFIAHARATPGKLNYGSSGPGSSPH
                     LATELFMSLTGVEMAHISYKGIGPAITALLANEVQFSLVPIAVGMPHVKAGKLRALGT
                     CGPQRSAATPDVPTVNESGLPGFEVIAWWGMLAPAQTPPAVVGRLNREVRAILDLPEV
                     RRSMVDQGMDPAAGEPGEFAALIKADMEKWGDIGKRLGVKLD"
     gene            complement(2246..2833)
                     /locus_tag="VIG70_20370"
     CDS             complement(2246..2833)
                     /locus_tag="VIG70_20370"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY8252956.1"
                     /translation="MFDLGGVVVRWDPDAIIAGVFKDEDKRARVREGVFAHPDWLELD
                     RGTLGRDDAITRAAQRTGVPVPDIRRLLHAVPPSLVVFPETVDLLHRLKRKGYPLYCL
                     SNMHFASIEYLEKTHTFWDVFDGAVISCRLQLCKPEAGIYEHLLRTHGLKAQDTLFID
                     DVQKNLDAAAQLGIRTLRFENAAQCERELRALGVL"
     gene            complement(2839..3846)
                     /locus_tag="VIG70_20375"
     CDS             complement(2839..3846)
                     /locus_tag="VIG70_20375"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY8252957.1"
                     /translation="MLVPHEKLAAFTAACLEHLGLEDADAKLVAETLVAADLRGVDSH
                     GVVRLPHYARRLRNGSVKARPSISVQRTSASAAVVEGDAGMGQLVAQRAMNEAIKLAL
                     ETGIGAVVARNSSHCGACAWFVELAVQAGMIGVALTHTDSIMVPPGMKRIFLGSNPIA
                     FGAPGEREPVIIDMSTTHVAWGKVLVARQEGKSIPPDWGVDPDGNPTTDPQQVVGLAP
                     TGGHKGYALAAMIEILCAQLAGVPFGRHVTQMYGELNKPRNLGHFMLALDPARFAQRK
                     AFLSQIALFLDEIRAEGAMAPGDPERRTAAQRRREGIPLGEATVEELNRLADEAGVDK
                     L"
     gene            3887..5179
                     /locus_tag="VIG70_20380"
     CDS             3887..5179
                     /locus_tag="VIG70_20380"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY8252958.1"
                     /translation="MAKKKDGARPDDIDPKHRWDRPLQAPGRTQVDFEERVDFRRLHD
                     YRLARTRAALAHSGLGALLSFDQHNIRYTTSTVIGEWARDKLTRYSLLTGNGEPYIWD
                     FGSAARHHKLYAPWLKSDHCRAGLLGMRGAVGSDLFLFKGAAKEIKAILKAEGVANLP
                     LGVDVVEPPMLFALQDQNIEVRDGQQTMLQAREVKNVDELTLLNMAAAMVDGVYQDIA
                     EALKPGVQESQIVGLATKRLYEMGSDCVEAINSISGERCSPHPHNFTDRLIRPGDQAF
                     FDIIQSFMGYRTCYYRTFNVGRATGPQRTAYKKAREWMDRAIELLKPGMSSDKIAVAF
                     PEAKEIGFASEMDAFGLNFCHGLGLGLHERPLISRLTSLREPIELKAGMVFAVETYCP
                     ATDGISAARIEEEVILTPKGAKIISLYPAEELPIANPY"
     gene            5163..6095
                     /locus_tag="VIG70_20385"
     CDS             5163..6095
                     /locus_tag="VIG70_20385"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8252959.1"
                     /translation="MPTPTKQTLGWIGTGRMGYAMAERLAKGGCDIAVWNRTKAKAEP
                     LTKHGAKVVNQLEELSTKDILFVMVSTYEDVKEVIGKTLAAGGNAGKPKMVVECSSIS
                     LEGSAELRKNLAGKGVEYLAAPVSGNAKVIKAGKLTFVVSGPKAAYEKAKPYLDMMGT
                     GSSYVGEGELARIAKICHNVMLGVVIQNLCEITILAQKAGMPRHAFLDFLNKSVMGSM
                     FTRYKTPALVNLDFHVTFTPKLLRKDLDLGLDAARNLEVPMPLSSLMRDLLQQMIGQG
                     MTEQDFSALILAQAKASGLELKPENVQVGDGLSS"
     gene            6100..7230
                     /locus_tag="VIG70_20390"
     CDS             6100..7230
                     /locus_tag="VIG70_20390"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252960.1"
                     /translation="MILLSPGGTAVQRQAAPAARLVVGTVEGIFVFERDAGRHWFPAG
                     RALEGCFVSALTRLESGALIAATHGLGVVRSDDGGMTWRLANAGLSHFDVWVVKAERL
                     GGRERLFAGTLPAHLFVSEDGAASWRELTALRDVPSAPQWSFPPPPHQGHVLDVASLG
                     DSLYVGIEVGALLRSDDGGKTFSELPVNPDVSEVDIHRLALHPKRPQRIIAATGWGMI
                     ASDDRGASWRKGERLPGIDYPIPLVAHPDDPDLLFVAGGEGWPPNWYQIGRSRAKIAR
                     SRDGGRSWQRLLGGLPDGQRPTFGGLALEAWDGGFAVLAADTDGQIYESRDGGDRWSV
                     VAEAAPVSKGEQYRGLAKGRPPLVGTDSLVFAEAGQRRVSQA"
     gene            7243..7533
                     /locus_tag="VIG70_20395"
     CDS             7243..7533
                     /locus_tag="VIG70_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252961.1"
                     /translation="MRLLLTSLMIGALAIAAGCAGNPNSSLARQCESGLSIAYKELDF
                     AKAKGFGGTVEYSQAAGLLTAAKVQFEFGKYPNCIDKVDRARAFITRSQTGG"
     gene            7630..8811
                     /locus_tag="VIG70_20400"
     CDS             7630..8811
                     /locus_tag="VIG70_20400"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEY8252962.1"
                     /translation="MYTATSGVVLPTTIIGSLPRPGWYTQNLGRREFREAMVERSYRE
                     QYLDAVSTYLRDQEVAGLDIVTDGDCRFDADVAGHNWFSYAPLHMSGFDGANFYRAGG
                     KAGFPHKPGHILHDVLETRVMPDLVGPVGRGALQYTAIWKAAQRQTTKPVKFGTIMPE
                     LIAMAVRDLHYKDLRKAILAISDALNEELHELADAGCKVIQMEEPQIHLLAAKGLVDK
                     VLNPDFMVEVFNNTVRGLRNKTEIWCHTCWGNPAAQRLFAKTPSYAPALEPLSRIDAD
                     VITFETCSSGGMDLEAIGKHIRSQKVCIGVVDHHTLQVETSDQVAALVRKALEHIPAE
                     RLILGSDCGMGREGMSRRHAFYKMVSIVQGANVVRRERKLPEAPCLAADERFSMVERE
                     G"
     gene            8811..9860
                     /locus_tag="VIG70_20405"
     CDS             8811..9860
                     /locus_tag="VIG70_20405"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HEY8252963.1"
                     /translation="MKKVIVFALIAVAPLVVDARDYKMTIGSSHPPVLPWTIPLKTLV
                     VPESNKRLKALGLPDTIQWTEAYAGALYDFNNTLEGVGDGLADIGWVGSLWEPAKQPL
                     QNVAYYSPFVTGDFRALVEVQNQLMEKVPAFAAEWTKHNTVFLGAQVADTYHLVTKFP
                     VKSLEDIKGKKLLAPGAIASRVHGTGAIPVDAGLPVFYNMLQTGVADGAVILITGVLP
                     FKLHEVAPYITKVDLGAVFSGALAMNKKTWDGLPPHMKDLFRQIGRDYAKMCADLVAE
                     REASTWAALGKMPNVTVSELSQAERAKWAKALPDVAGDWARRNGDAGRQVLKIFMEEA
                     RKHGAKPLRDWDRGL"
     gene            9905..11755
                     /locus_tag="VIG70_20410"
     CDS             9905..11755
                     /locus_tag="VIG70_20410"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY8252964.1"
                     /translation="MNSVGTLWVFALMFLICADVAGRYLLSAPIKGAAEMVGYSIVTA
                     VFLQMASTLRAGRFTRVELLIEPLEARCPAAGHAFNALYHLVGAAVFAVITWGTWPKL
                     REAWANDEITGTPGVFVFLIWPFLAVIVFGAAVTALEFLIQLAGAAGRAARSWRAGSP
                     WPPLALGVAVVAALAGLFSFSDLTNAQIGIFGVGCMVVLLIGGLPIGVTMIVVGFGGI
                     WLIRGDPGLGMRTLSQSSTEYLNSYFFGVVPLYVLMGLLISTSDVGRDTFAVARWLLR
                     RVRGGLGVATVAANAVFAAVTGSSIASAAVFTKIATPEMLHHGYTPRFSVGVVAGSSV
                     LGMLIPPSLLFIVYGFLAEQSVGDLFLAGVVPGLVLAAAFAAMIVLMATFWPAQVGGH
                     KVEEEGEESVRSAALKVMPVVALVVIVLGGIYGGIFTPTESGAAGALGALVIALARRK
                     LGWRRLWDTMVETGYVTVSILFLILGARIFGQMLAMSGLPQHLSALAAGAGFGLVGFV
                     LLYVALAVVLGCMLDSVSIMVITLPFAIPVVKALGGDLIWFGVVAVVAVEIGLLTPPF
                     GISVYVVKSTLDDQRIALRDIFAGAFPFVVCMALVTLLLVFVPQLSLVFK"
BASE COUNT         1894 a         3932 c         4060 g         1930 t
ORIGIN      
        1 ggccgcggcc gcgccggcca agacacccaa ggacgtggtc acgcggctgc acggcgcgat
       61 ggtcaaggcg ctgcgctcgc cggatatcgc cgagcgcatg aaccagatcg gcttcgacgt
      121 ggtggcgagc tcgccggaag aattcggcgc cttcatgaag gccgaggtcg agcgctggac
      181 gcgcgtcgtg agcaagggcg gcatcaaacc cgactgatgg gtttggtttt cagaaaggag
      241 aatctgaccc gggcgatcga ggcgatcgcg ctcgccggcg gcagcgagcc ggcggaagcg
      301 aagctggtcg ccgagaacct ggtcaccgcc aacctgatgg gacacgactc gcacggcatc
      361 ggcatgatgc cgcgctacgt cgaggcgttg ctcgagggcg gactgcatcc gaaccggcag
      421 gtcgaggtga agttcgatgc cggtgcgctg ctcacgctcg atggctgcgc cggctacggc
      481 cagtcggttg gcgtgcaggc gacgcaaatg gcgatcgcgc gcgcgcgcga gcacggcacg
      541 gcgatcgtcg cgctcggcaa ttctcaccat ctcggacgca tcggccactg ggccgagatg
      601 gcggtcgcgc aaggcctggt gtcgctgcac ttcgtgaacg tccagtcctt cgcgcgcgtc
      661 gcgccgttcg ccggcgccga ccggcgcttc ggcaccaatc cggtgtgcat cggcattccg
      721 ctccccggcg agccgccgtt cctgctcgac atggcgacca gcgcagtcgc ccagggcaag
      781 ctgcgcgtgg cgcacaacaa gcgcgccaag attccaccgg gctggctgat cgacgaccag
      841 ggaaacccga ccgacgaccc gcgctggggt gtggtggcgc cgctcggagc gatggcgtgc
      901 ttcggcgagc acaaggggta cggcctggcg gtggcgtgcg agctcctcgg cggcgcgctc
      961 accggcggcg gcgttaccga ctacgacaac aagacgcagc ggcgcgtgct gaacggcatg
     1021 atctccatcg tgatcgatcc ggcgcgcctc ggcacccagc aggcgttcgc aaaagacgcg
     1081 aagagctttc ttgtttggct gcgcgcctcg cgcgccgcac ccggccacga ccgcgtgcgc
     1141 atcgccggcg agcccgagcg cgagtaccgc gcgaaacgcg agcgcgacgg cgttccggtg
     1201 gacgaggaga cctggaagga gatccgcgcc gccgcggcga agctgaagct ctcgccggag
     1261 cgcatcgacg cgctggcgcg aggaggctag ccatgcgccg ttcgtttctg gcaatcgttc
     1321 ttctggtcgt ctccgccgcg ctcggtgcgc aggagtaccc cgttcgcacg gtgcgcatcg
     1381 tggtgccctt cgcccccggc ggcggcaccg acctgatcgc gcgcgtcctg tcgcagcacc
     1441 tgagccagcg gctcgggcag agcttcgtgg tcgagaaccg cccggcgggc tcgggcatcg
     1501 tcggcgccga ccatgtggcg aagagcgcgc ccgacggcta cacgctgctc ttcgcgttct
     1561 cctcgctttc gagcagcgcc aagctcttca ccaagctgcc ctacgatccg gtgcgcgact
     1621 tcgcgccggt cgcgctcgcg acgacctcgc cgctgctcgt cgtggtgccg gccgcgctgc
     1681 cggcgaagga cttgcgcgag ttcatcgccc acgcccgcgc gacgccgggg aagctcaact
     1741 acggctcgtc gggccccggc agctcgccgc acctcgcgac cgagcttttc atgtcgctca
     1801 ccggcgtgga aatggcgcac atctcctaca aggggatcgg gcctgcgatc accgcactgc
     1861 tcgcgaacga ggtgcagttt tcgctggtgc cgatcgcggt cggcatgccg cacgtgaaag
     1921 ccgggaagct gcgcgcgctc gggacctgcg ggccgcagcg ctccgcggcg acgcccgatg
     1981 tgccgacggt caacgagtcc gggctgcccg gattcgaggt gatcgcgtgg tggggcatgc
     2041 tcgcgccggc gcagacgccg cccgcggtcg tcggccggct caaccgggaa gtgcgcgcca
     2101 tcctcgacct cccggaagtg cggcgcagca tggtcgatca gggcatggat cccgcggccg
     2161 gcgagcccgg cgaatttgct gcgctgatca aggccgacat ggaaaagtgg ggcgacatcg
     2221 gcaagcggct cggggtgaag ctcgactaga gcacgccgag cgcgcgcagc tcgcgctcgc
     2281 actgcgccgc gttctcgaac ctcagcgtgc ggatgcccag ctgcgccgcg gcgtcgaggt
     2341 tcttctgcac gtcgtcgatg aacagggtgt cttgggcctt caggccgtgc gtgcgcaaga
     2401 ggtgttcgta aattccagct tcgggcttgc agagctgcag ccggcacgaa atcaccgcgc
     2461 cgtcgaatac gtcccagaac gtgtgcgtct tctcgagata ttcgatcgag gcgaaatgca
     2521 tgttcgacag gcaatagagc gggtatccct tgcgcttcag gcggtggagc agatcgacgg
     2581 tttccgggaa cacgaccagc gacggcggca ccgcgtgcag cagccgcctg atgtctggca
     2641 ccggtactcc agtgcgctgc gctgcgcgcg tgatcgcgtc gtcgcgcccc agcgtgccgc
     2701 ggtcgagctc gagccagtcg gggtgggcga agactccctc gcgcactctc gctcttttat
     2761 cctcgtcctt gaaaactccg gcgatgatcg cgtccgggtc ccagcgcacc accacgccgc
     2821 cgagatcgaa caccagattc aaagcttgtc gactcccgct tcgtcggcga gccggttcag
     2881 ctcctcgacg gtcgcttcgc cgagcgggat gccctcgcgc ctgcgttgcg ccgcggtgcg
     2941 gcgctccgga tcgccgggcg ccatcgcgcc ctcggcccgg atctcgtcga ggaaaagcgc
     3001 gatctgcgaa aggaacgcct tgcgctgcgc aaaccgcgcc gggtcgagcg cgagcatgaa
     3061 gtggccaagg ttgcgcggct tgttcagctc gccgtacatc tgggtgacgt gccggccgaa
     3121 aggcacgccg gcgagctgcg cgcacaggat ctcgatcatc gccgcgagcg cgtagccctt
     3181 gtggccgccg gtcggggcga gcccgacgac ctgctgggga tcggtggtcg gattgccgtc
     3241 ggggtcgacg ccccagtcgg gcggaatcga tttcccctcc tggcgcgcga ccagcacctt
     3301 gccccacgcg acgtgggtgg tcgacatgtc gatgatcacc ggctcgcgct cgccgggggc
     3361 gccgaaggcg atcgggttcg agccgaggaa gatgcgcttc atccccggcg gcaccatgat
     3421 cgaatcggtg tgcgtgagcg ccacgccgat catgcctgcc tggacggcga gctcgacgaa
     3481 ccaggcgcag gcgccgcagt gcgagctgtt gcgggccacg actgctccaa tgcctgtttc
     3541 cagggccaac ttgatcgctt cgttcattgc gcgctgcgcg acgagctggc ccattccggc
     3601 gtcgccttcg accaccgccg ccgaagccga cgtgcgctgc accgagatgc tcgggcgcgc
     3661 cttcacgctg ccgttgcgca gccgcctcgc atagtggggg aggcgcacta cgccatggct
     3721 gtcgacgccg cgcaggtctg ccgcgaccag cgtctcggcc acgagcttgg cgtccgcgtc
     3781 ctcgagcccg aggtgttcca ggcacgcggc ggtgaaagcg gcgagcttct cgtgaggtac
     3841 cagcacgcgg agaattctag atagaatcgg cgcgcgagga ggcaggatgg cgaaaaagaa
     3901 ggacggcgcg cgtccggacg atattgaccc gaagcatcgc tgggaccggc cgcttcaagc
     3961 gccgggtcgc acgcaggtgg atttcgaaga gcgcgtcgat tttcgccgcc tgcatgacta
     4021 ccgcctcgca cgcacgcgcg cggcgctagc ccactccggg cttggcgcgc tgctctcgtt
     4081 cgaccagcac aacatccgct acaccacgag caccgtgatc ggcgaatggg cgcgcgacaa
     4141 gctcacgcgc tactcgctcc tcaccgggaa cggcgagccc tacatctggg acttcggcag
     4201 cgccgcgcgg caccacaagc tctacgcgcc gtggctgaag agcgaccatt gccgtgccgg
     4261 cctccttggg atgcgcggcg ccgttggctc ggacttgttc ttgttcaagg gagcagccaa
     4321 ggagatcaaa gcgatcctga aggccgaggg cgtggcgaat ctgcccctgg gcgtggacgt
     4381 ggtcgagccc cccatgcttt ttgcattaca agatcaaaat attgaagtac gcgatgggca
     4441 gcagacgatg ctgcaagcgc gcgaggtaaa gaacgtcgac gagctgacgc tgctcaacat
     4501 ggcggcagcg atggtcgacg gcgtctacca ggacatcgcc gaggcgctga agcccggcgt
     4561 gcaggagtcg cagatcgtgg ggctcgccac caagcgcctc tacgagatgg gctcggactg
     4621 cgtcgaggcg atcaactcga tctcgggcga gcgctgcagc ccgcacccgc acaacttcac
     4681 cgaccggctg atccgtccgg gggaccaggc gttcttcgac atcatccagt cgttcatggg
     4741 ctaccgcacc tgctactacc gcaccttcaa cgtcgggcgc gccaccgggc cgcagcgcac
     4801 cgcgtacaag aaggcgcgcg agtggatgga ccgggcgatc gagctcctga agcccggcat
     4861 gtcgagcgac aagatcgccg tcgccttccc ggaggcgaag gagatcggct tcgcgagcga
     4921 gatggacgcg tttggcctca acttctgcca cggcctcggc ctcgggctgc acgagcggcc
     4981 gctcatctcg cgcctcacct cgctgcgcga gccgatcgag ctgaaggccg gcatggtgtt
     5041 cgccgtcgag acctactgtc ccgcgaccga cggcatctcc gccgcgcgca tcgaggagga
     5101 ggtcatcctc acaccgaagg gcgccaagat catcagcctg taccccgcgg aggaattgcc
     5161 aattgccaac ccctactaag cagacgctcg gctggatcgg caccggccgc atgggctacg
     5221 ccatggccga gcgcctggca aagggcggct gcgacatcgc ggtgtggaac cgcaccaagg
     5281 cgaaggccga gccgctcacg aagcacggcg cgaaagtggt caatcagctc gaggagctgt
     5341 cgaccaagga cattcttttc gtgatggtct ccacctacga agacgtgaaa gaggtgatcg
     5401 gcaagacgct ggccgcaggt ggcaatgcgg gcaagccgaa gatggtggtg gagtgctcgt
     5461 cgatctccct cgaagggtct gcagagctcc gaaagaatct tgccggcaaa ggcgtcgagt
     5521 acctcgcggc gcccgtgagc ggaaacgcca aggtcatcaa ggccggcaag ctgacgttcg
     5581 tcgtgtccgg gccgaaggcc gcgtacgaga aagcgaagcc ctatctcgac atgatgggca
     5641 ccggctcgag ttacgtgggc gagggcgaac tcgcgcgcat cgccaagatc tgccacaacg
     5701 tgatgctcgg ggtggtgatc cagaacctgt gcgagatcac gattctcgcg cagaaggccg
     5761 gcatgccgcg ccacgcgttc ctcgacttcc tcaacaagag cgtgatgggc tcgatgttca
     5821 cgcgctacaa gaccccggcg ctggtgaacc tcgacttcca cgtcacgttc acgccgaagc
     5881 tgctgcgcaa ggacctcgac ctcggcctcg acgccgcgcg caatctcgag gtgccgatgc
     5941 cgctcagctc gctgatgcgc gatctgctgc agcagatgat cgggcagggc atgaccgagc
     6001 aggacttctc cgcgctgatc ctcgcgcagg cgaaggcctc cggcctcgag ctcaagccgg
     6061 agaatgtcca ggtgggagac gggctctcct cctgaggcca tgatcctcct gtcgcccggc
     6121 ggcacggcgg tccagcggca ggcagctccc gcggcgcggc tcgtcgtcgg caccgtggag
     6181 ggcatcttcg tgttcgagcg cgatgccggg cgtcactggt tcccggcggg acgtgccctg
     6241 gaggggtgtt tcgtctccgc gctcacgcgg ctcgaaagcg gcgcgctgat cgccgctacc
     6301 cacggcctcg gcgtggtgcg cagcgacgac ggcggcatga cctggaggct cgccaacgcg
     6361 ggcctgagtc acttcgacgt ctgggtggtg aaagccgagc gcctcggcgg ccgcgagcgg
     6421 ctgttcgccg ggacgctgcc ggcacacctc ttcgtgagcg aagacggcgc ggcgagctgg
     6481 cgcgagctca ccgcgctgcg cgacgtgccg agcgcaccgc agtggtcgtt tccgccgccg
     6541 ccccatcagg ggcacgtact cgacgttgcc tcactcggag attccttgta tgtgggcatc
     6601 gaggtgggtg cgctacttcg ctcggacgac ggcggcaaga ccttcagcga gctgccggtg
     6661 aatcccgacg tcagcgaggt cgatattcac cgcctggcgc tgcatccgaa gcggccacag
     6721 cgcatcatcg ccgcgaccgg ctggggcatg atcgcgagcg acgatcgcgg cgcgagctgg
     6781 cgcaagggcg agcgccttcc cggaatcgac tacccgattc cgctggtggc gcatccggac
     6841 gacccggacc tgctgttcgt cgccggcggc gagggctggc cgccgaactg gtaccagatc
     6901 ggccgctcgc gcgcgaagat cgcgcgcagc cgcgacggcg gacgcagctg gcagcgtctg
     6961 ctcggcggcc tgccggatgg gcagcgtcct acattcggcg ggctggctct ggaggcgtgg
     7021 gacggcggct tcgcggtgct ggcggccgac accgacggcc agatctacga aagccgcgac
     7081 ggcggcgatc gctggagcgt ggtggccgaa gccgcgccgg tatcgaaggg agagcagtat
     7141 cgcggtctgg cgaagggacg gccgccgctc gttgggacgg attccctcgt tttcgccgaa
     7201 gcgggacaac ggcgcgtaag ccaagcctga gctggcggag ccatgagact actgctcact
     7261 tccttgatga ttggtgctct tgcgatcgcc gcgggctgcg ccggcaatcc gaacagctct
     7321 ctggcgaggc agtgcgagag tggcctcagc atcgcctaca aggagctcga cttcgccaag
     7381 gccaagggct ttggcggcac cgtcgagtac tcgcaggcag cgggtttgct caccgcagca
     7441 aaggtgcagt tcgagttcgg caagtacccc aactgcatcg acaaggttga tcgggcgcgg
     7501 gcctttatca ccaggtcaca aaccggcggc tgatcccgcg caagcgactg cgcgaagcgc
     7561 catcgggctg atgggacagt tccctcggcc cttcctaagg tagcatccgt cccgaggagg
     7621 aggaggtcga tgtacaccgc gacatcgggc gtcgtgctgc cgacgaccat catcggctcg
     7681 ctgccgcgac cgggctggta cacccagaat ctcggccggc gcgaatttcg cgaggcgatg
     7741 gtcgagcgca gctatcgcga gcagtacctc gacgcggtct cgacctatct gcgcgaccag
     7801 gaagtcgccg gcctcgacat cgtcaccgac ggcgactgcc gcttcgacgc cgacgtcgcc
     7861 ggccataact ggttctcgta cgcgccgctg cacatgagcg gcttcgacgg cgcgaacttc
     7921 taccgcgcgg gcggcaaggc cggcttcccg cacaagcccg gccacatcct gcacgacgtg
     7981 ctcgagacgc gcgtcatgcc cgacctcgtg gggccggtcg ggcgcggcgc gctgcagtac
     8041 accgcgatct ggaaagccgc gcagcgccag acgaccaagc cggtcaagtt cggcaccatc
     8101 atgcccgagc tcatcgccat ggcggtgcgc gaccttcact acaaggactt gcgcaaggcg
     8161 atcctcgcca tcagcgacgc gttgaacgag gagttgcacg agcttgcgga cgcgggctgc
     8221 aaggtgatcc agatggagga gccgcagatc cacctgctcg cggcgaaggg gctggtcgac
     8281 aaggtgctca atcccgactt catggtggag gtattcaaca acacggtgcg cgggctgcgg
     8341 aacaagaccg agatctggtg ccacacctgc tggggcaacc cggcggcgca gcgcctgttc
     8401 gccaagactc cgagctacgc cccggcgcta gaaccgctct cgcgcatcga cgccgacgtc
     8461 attaccttcg agacctgcag ctccggcggc atggacctcg aggcgatcgg aaagcacatc
     8521 cgcagccaga aagtctgcat cggcgtggtc gaccaccata ccctgcaggt cgagacgtcc
     8581 gaccaggtcg cggcgctggt gcgcaaggcg ctcgagcaca ttccggccga gcgcctgatc
     8641 ctcggctccg attgcggcat ggggcgcgag ggcatgagcc gccgccacgc gttctacaag
     8701 atggtgtcga tcgtgcaggg cgcgaacgtg gtgcggcgcg agcgcaagct gccggaggcg
     8761 ccgtgcctcg cggccgacga acgattttcc atggtggaga gggagggctg atgaaaaagg
     8821 taattgtgtt tgcgttaatc gccgtcgcgc ccctggtggt cgatgcccgc gattacaaga
     8881 tgaccatcgg ctcgagccat ccgccggtcc tgccgtggac gatcccgctc aagaccctgg
     8941 tcgtcccgga gtcgaacaag cggctgaagg cgcttgggct gccggacacc atccagtgga
     9001 ccgaggccta cgccggggcg ctctacgatt tcaacaacac gctcgaaggc gtgggcgacg
     9061 gcctcgccga catcggctgg gtaggctcgc tctgggagcc ggcgaagcag ccgctgcaga
     9121 acgtcgccta ctactctccc ttcgtcaccg gcgacttccg cgccctggtc gaagtgcaga
     9181 accagctcat ggagaaggtg ccggcgttcg ccgcggagtg gaccaagcac aacaccgtgt
     9241 tccttggggc gcaggtggcg gacacctacc acctggtcac caaattcccg gtcaagagcc
     9301 tggaggacat caaagggaag aagctgctgg cgccgggcgc catcgcgagc cgcgtgcacg
     9361 gcaccggagc catcccggtc gacgccggac ttccggtgtt ctacaacatg ctgcagaccg
     9421 gcgtcgcgga cggcgccgtg atcctgatca ccggcgtgct cccgttcaag ctgcacgagg
     9481 tcgcgcctta catcaccaag gtcgatctcg gcgcggtgtt ctcgggggcg ctggcgatga
     9541 acaagaagac ctgggacggc ctgccgccgc acatgaagga tctgtttcgc cagatcggcc
     9601 gcgactacgc caagatgtgc gccgacctcg tggcggagcg cgaggcatcg acctgggcgg
     9661 cgctcggcaa gatgccgaac gtgacggttt ccgagctgtc gcaggccgag cgcgcaaagt
     9721 gggcgaaggc gctgcccgat gtcgccggcg actgggcgcg gcgaaacggc gacgccggcc
     9781 gccaggtgct gaagatcttc atggaggaag cgcgcaagca cggcgccaag cccttgcgcg
     9841 actgggatag aggtctctga aacccggccc cctgagcagg gggttcggcg cgctgacgct
     9901 ggtcatgaac agcgtgggca ccctgtgggt gttcgcgctg atgtttctca tctgcgccga
     9961 cgtcgccggc cgctatctcc tgagcgcgcc gatcaagggc gcggccgaga tggtcggcta
    10021 ctcgatcgtc accgccgtgt tcctgcagat ggcgagcacc ctgcgcgccg ggcgcttcac
    10081 ccgtgtcgag ctgctgatcg agcctctcga ggcgcgatgc ccggccgccg gccatgcctt
    10141 caacgcgttg tatcacctag tgggcgccgc ggtgttcgcc gtgatcacct ggggcacgtg
    10201 gcccaagctg cgcgaggcct gggcgaacga cgagatcacc ggcaccccgg gcgtgttcgt
    10261 gttcctgatc tggccgttcc tcgccgtgat cgtgttcggc gccgcggtga cggcgctcga
    10321 gttcctgatc cagctcgccg gcgccgcggg gcgcgcggca cgctcctggc gcgcgggctc
    10381 gccgtggccg ccgctcgcgc tcggcgtcgc cgtcgttgcc gcgctcgcag gcctgttctc
    10441 gttctccgac ctgacaaacg cccagatcgg gatcttcggc gtcggctgca tggtcgtgct
    10501 cctcatcggc ggcctgccga tcggcgtgac gatgatcgtg gtcggctttg gcggcatctg
    10561 gctgatccgc ggcgatcccg gcctgggcat gcgcacgctg tcgcagagct cgaccgagta
    10621 tctcaacagc tacttcttcg gcgtggtgcc gctctacgtg ctgatggggc tgctcatcag
    10681 cacgtcggac gtcggccgcg atacgttcgc ggtggcgcgc tggctgctgc ggcgggtgcg
    10741 cggcgggctg ggggtcgcga cggtcgcggc gaacgcggtg ttcgccgcag tcacgggctc
    10801 gtcgatcgcc tcggccgcgg tgttcaccaa gatcgccacc ccggagatgc tgcaccatgg
    10861 ctataccccg cgcttctcgg tcggcgtggt cgccgggtcc tcggtcctcg gcatgctgat
    10921 cccgccgagc ctgctattca tcgtgtatgg ctttctcgcc gagcagtcgg tcggcgacct
    10981 gttcctcgcc ggggtggtgc cgggactggt cctcgccgcg gcgttcgccg cgatgatcgt
    11041 gctgatggcg acgttctggc cggcgcaagt cggcggtcac aaggtcgaag aggaaggaga
    11101 ggagagcgtg cgcagcgcgg cgctcaaggt gatgccggtc gtcgcgctcg tcgtgatcgt
    11161 gctgggcggc atctacggcg gcattttcac gccgacggag tcgggcgccg cgggcgcgct
    11221 cggcgcgctg gtgatcgcgc tcgcgcggcg caagctcggc tggcgccggc tgtgggacac
    11281 gatggtcgag accggctacg tcacggtctc gatcctgttc ctgatcctcg gggcgcgcat
    11341 tttcgggcag atgctcgcga tgtcggggct gccgcagcac ctctcggcgc tcgccgccgg
    11401 cgccggcttc ggcctcgtcg gcttcgtgct gctctacgtg gcgctcgccg tggtgctcgg
    11461 ttgcatgctc gactcggtgt ccatcatggt gatcacgctg cccttcgcga ttcccgtggt
    11521 caaggcgctc ggcggcgatc tcatctggtt cggcgtggtc gcggtggttg cggtcgagat
    11581 cggactgctc accccgccgt tcggcatctc ggtctacgtg gtgaagtcca cgctcgacga
    11641 tcagcgcatt gcgctgcgcg acatcttcgc cggcgcgttc ccgttcgtag tctgcatggc
    11701 gctggtcacg ctgctcctcg tgttcgtgcc gcagctcagc ctggtcttca aatagccgcg
    11761 accagcagca gcccgccgat gacgccgcag ttcacgatca ggttggagag gtgcag
//
LOCUS       DATBSZ010000316        16822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_1024163, whole genome shotgun sequence.
ACCESSION   DATBSZ010000316 DATBSZ010000000
VERSION     DATBSZ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16822
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024163"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VIG70_20415"
     CDS             <1..654
                     /locus_tag="VIG70_20415"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY8252965.1"
                     /translation="GVYISPDGKLVSAAVELTNSVAFIDTASDTVVFRVKIKGDNPEH
                     AVFSPDGRFVYVSAEEDDKLEVIDVAKRALVTQLKVGTRPRGIAFTPDSAKAYVACER
                     ADTVYVVDARQHKILRTIKVGVRSNGLAMHPDGKRLYLSNGGAANVMVIDTGTDQVVA
                     TIPVGQRPWNMALSRNGAKLYVANGRSDSVSVIDTAKGERIKDIPVGKTPWGVHILD"
     gene            complement(662..2206)
                     /locus_tag="VIG70_20420"
     CDS             complement(662..2206)
                     /locus_tag="VIG70_20420"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY8252966.1"
                     /translation="MNRREFLAIGAASIGAACSAPAARRVPAAGASQAVPIGAVRRLI
                     VERVDPVLGRCRYAADVGGRQIVHPVLVARRGERFDAVIENRLPQPTTVHFHGLTLSE
                     AQDGAGFDAIAPGAAKALSFEVKNRSGLYWFHPHAHGFTAEQVYSGLVGLLVVVDAED
                     AALDAALALGPDNRLALAMSDVRAAAGSIRPYAPSAEDCLHGWYGNRALVNAALDVRR
                     TVAPGWVRLQLLNACNARGLLLAFRDADANVPFHLLGTDGGLLAAPVGVDRVFVYPGE
                     RVDLAIDASGRRELVAVSLAFDPRHQAKGGRLAHAHPARARYAPLAAETVCGERASSA
                     DALPDGAELGIFTLQVAGTGAGQLPPLPARLSTLPEPPQATVRRLRLDFDEHAGFLID
                     NTPYRADEIAFSVERGAREVWEVRNSPISMPHPMHLHGFGFRVLRRRGTFGPARALAG
                     EEGRLPTDLGVKDTVVVWPNETLWLAVDFALPDEAAFRGRQRYMFHCHNLEHEDGMMM
                     RNFAVL"
     gene            2352..4559
                     /locus_tag="VIG70_20425"
     CDS             2352..4559
                     /locus_tag="VIG70_20425"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HEY8252967.1"
                     /translation="MTSLKKLLHVFAGALLIGLTSGKAVAHDRDDDDRDRHRHHGDQQ
                     FQIQVLSGRPDTVAGGDALVRISAGKKDVRLSDVRVELNGRNITSAFVADPGAKTLTG
                     LVTGMRIGRNELEVDAKRKGHGRADADLVLTNHPIEGPVFSGPHEEPFFCQTHQFNMP
                     AGLGTLTPTQITDPCHIPMRVDYIYRTNATPSALAQWPAGATAYPANMAMTAKGKPYI
                     LRLETGTVNRSIYQIAMLHDPIAQPAAPTWSNRSANWNGRMVFNFGGGCPSGWYRQGA
                     NTGGVTDDFILSQGYALVSSSLNVAGNNCNNVTTAESMMMVKERFIERYGPPVHTQGF
                     GCSGGSYQQHEITDNYPGLLDGIIPGCSFPEVTFSLLTSLADAWLLDTYFKRSDIGAW
                     TTEQKRQVSGFLKFESIAAGAAIANRIDPDGNCGVVPVEMRYNAVTNPTGVRCTIYDH
                     AVNIYGTDPATGFARRPLDNEGIQYGLKLVNSGAISVEQFLSLNEKIGGFDKDANIVP
                     QRTQADLLATRISYRTGRMTNAGAGLAAVPIIDYRAYVDNATNGEVHLAYHSFSMRKR
                     LENENRRSDNQVMVLEDSRFGLYSSASPLLQRMVTTMDRWITAIKADKRRMSMADKIA
                     ANKPADLLEGCNTGGTSPTFMPQTLVREQEAATACNQLYPSNSFPREVAGADIAADII
                     KCQRRAPRQSDYAVTFTSAQWARLQAIFPTGVCNWSRPGYEQQDLKGTWLDFD"
     gene            complement(4568..6481)
                     /locus_tag="VIG70_20430"
     CDS             complement(4568..6481)
                     /locus_tag="VIG70_20430"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEY8252968.1"
                     /translation="MRPLWTPSPERAAASELARFMRLAGKPSYEELHRWSIESSAEFW
                     NRVWDFCAVEGKKGSQTVVNPERMPGAKWFPEAKLNYAENLLRERDASDAIVFWGEDR
                     IKRRINHFNLYQLVSRISQALADAGVRKGDRVAGYLPNVPEAAAALLATASLGAVWSS
                     CSPDFGVQGVLDRFGQIEPKILFCADGYLYGGKEFDSQEKASQVLEKLPSVEECVVVD
                     YLGTPVTAGTSLYDFLEPFDPAEIRFEQVEFDHPLYILYSSGTTGVPKCIVHGTGGAL
                     LQHLKEHRLHSDVKPGDRLFYFTTLGWMMWNWLVSGLASGATLLLYDGSPFVGRGNLM
                     FDLADAEGMTHFGTSAKFIDALAKTGLKPKETHRLAKLRAILSTGSPLAPEGFDYVYA
                     NVKEDVCLSSISGGTDIVSCFVLGNPMGPVWRGEIQAKGLGMAVEVFNEQGKPVKGEK
                     GELVCTKPFPSMPVGFWNDPDGAKYRAAYFEKYPGVWRHGDWCEETAHGGIVIYGRSD
                     AVLNPGGVRIGTAEIYRQVESLDEVAESLVIGQDWQGDVRVVLFVKLKDGLSLDDALT
                     ARIRSHVRANTTPRHVPAKILQVGDIPRTKSGKIVELAVRDVVHGRAVKNLEALANPE
                     ALEHFRSRPELGD"
     gene            complement(6478..6873)
                     /locus_tag="VIG70_20435"
     CDS             complement(6478..6873)
                     /locus_tag="VIG70_20435"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY8252969.1"
                     /translation="MNEKKLVHTERIAVRWGDMDAMGHVNNTVYFRYMEQARIAWFES
                     LVPRAEAWGETSIVIVNASCNFRRPINYPGTVEVKVFAGSPGGSSVGTFYELNVNGET
                     YADGAATVVFLDREQKPLRIPDRIRSLMQ"
     gene            6900..8525
                     /locus_tag="VIG70_20440"
     CDS             6900..8525
                     /locus_tag="VIG70_20440"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEY8252970.1"
                     /translation="MARESMQYDVVVVGGGPAGLAAAIRLKQRTPDRSVCVLEKGSEI
                     GAHILSGAVLDPRALSELIPDWKEKGAPLNTPVSRDRFLFLTQNSRFEVPAFALPACF
                     KNHGNYVVSLGNVCRWLGQQAEALGVEIFPGFAAAEVLYEGNAVKGVATGDMGVSRTG
                     EKTGAYQPGMELHGKYTLFAEGCRGHLGRQLEARFGLREGADPQVYGIGLKELWEVKP
                     ERHEPGLVVHTAGWPLDADTYGGSFLYHMDERLVAVGFVVGLGYENPYLSPYEEFQRF
                     KTHSAIRSFFEGGKRISYGARAIAAGGLQSLPRLVFPGGALIGDEAGFLNASRIKGSH
                     CAIKSAMLAADAAHDALAAGRAGDELAAYPEAFRSSWLHDELHRARNFKPWMSKGLYT
                     GTLMVGIDQVLFRGKAPWTLHHHRGDHEQTRPAAEFSPISYPKPDGALTFDRLSSVFI
                     SNTNHNEDQPVHLTLKDASVPVKVNLSRYAGPEQRYCPAGVYEFVDLDTKPRLQINAQ
                     NCVHCKTCDIKDPPQNIVWVAPEGGGGPNYSNM"
     gene            8666..8860
                     /locus_tag="VIG70_20445"
     CDS             8666..8860
                     /locus_tag="VIG70_20445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252971.1"
                     /translation="MNDEQQDFAVQLLLIEREASFVLEDLRPGVARDRIQHIVTIASL
                     LRARLDVASSLILPRPPMKD"
     gene            complement(9317..11548)
                     /locus_tag="VIG70_20450"
     CDS             complement(9317..11548)
                     /locus_tag="VIG70_20450"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8252972.1"
                     /translation="MATILVADDNRANREALAALLETAGHHVVRAIDGREALERAQEA
                     RPELVISDVLMPMMDGYELARRLKADPVTATAALIFYTAYFAGQDAQALAQAHGVSRV
                     LLKPSENAEILRQVDAALASRNEAPAEILGDLDQEHLRVMVDKLLEKTGALEAQQRRI
                     ERLNRTLEVLSAVNALIARAGDRQALLDEACRIAVDKGGFRLAAIGLADPLTHAIRQV
                     ASAGEGSAERDLQRLKLGTRSLIWNDAQSGSFVALPLLAGGETAGILLLYARQRDFFD
                     EEEMRLLHELAGDIAFALRHLAQKARMDYLAYHDSLTDLPNRSLFTDRMTQALNAARR
                     EKRFAAAIFVDVERFRMVNESFGRKAGDDLLREVGARLRQAAREQDTVARVGADHFAI
                     AVAPFDRPGDTAHAFLESLDQALAEPIVIEGVELRVSLKAGIAVFPTDGESTEALCAN
                     AETALIKAKQSGSHYLFYAPEMNARVAESLDMENRLRRALADGTLALHYQPKIEVGTG
                     AVAGLEALIRWNDPELGSVPPARFVSLLEETGMILAAGRWALRKTVEDIRRWQGLGLQ
                     VPRTSVNVSALQLRQKDFVDSVLDAICEFDGKAPLLDIEITESVLVDDIEESTRKLQA
                     LRRAGVEVSVDDFGTGFCSLSYLARLPVDTLKIDRSFVVRMRDAGYPRNIVAMIVSLA
                     HTLGLRVVAEGVEDDEQVRLLRELGCDQIQGFYVSRPVPPEDVDAMLSADVNTLKRRL
                     NAA"
     gene            11683..11904
                     /locus_tag="VIG70_20455"
     CDS             11683..11904
                     /locus_tag="VIG70_20455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8252973.1"
                     /translation="MALKPNARTCRIREQTVEDLPSGLTLHFQACGGDGARLVIAGKV
                     LPCGSHEIVFDTEGRTVRVGPVQLKKLPL"
     gene            11935..12249
                     /locus_tag="VIG70_20460"
     CDS             11935..12249
                     /locus_tag="VIG70_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEY8252974.1"
                     /translation="MGESAAGYRRFADFYPFYLSQHTDRTCRRLHFAGTTLGLLALLH
                     AFSTLNFWWLLAGLAAGYAFAWVGHFVFEKNRPATFTHPLYSFMGDWVMWKDMLTGRI
                     RL"
     gene            12246..13769
                     /locus_tag="VIG70_20465"
     CDS             12246..13769
                     /locus_tag="VIG70_20465"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY8252975.1"
                     /translation="MNGTASPFGFWTSAAPWLALVAFGLAVALLRARPAERATYLNTL
                     WLFLVGIGGEAAASLIWALNFVDAAGTVHAISRFVASIALIRLCGFAAFRLLLPLAGS
                     RPPRIAEDLTIVAVYVVYGFAQLRGAGLDLSSIVTTSAILTAVIAFAMQDTLGNVLAG
                     VAVQLDNSVRIGDWIRVDTVSGRVRDIRWRSTLIETRNWETVVIPNSMLMKARVEILG
                     QRDGAPLQWRRVLDFMVDPGVPPARVIAIVEDDMRELSIPNVARTPAPSCVLKGFEEG
                     NLRYQLRYFLTEINEDDLTDSMVRVHLFASLQRAGIRVAEPQRTVHAVQRDEAHAETV
                     RRRELTRRLQALASVPPFAKLPEETKAEIAERLQYAPFARGDVMTKQGNKAHWLYILA
                     YGEAEVLYEPPSGAPSVIGTVRAGEFFGEMALITGDARTATVVAKTDVECYRLDRASF
                     QEFLLGRAEISDDITRVMGGRRGDLERVRQAFATTVPVAEAEVRLATKIRRFFGMRT"
     gene            13811..14281
                     /locus_tag="VIG70_20470"
     CDS             13811..14281
                     /locus_tag="VIG70_20470"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8252976.1"
                     /translation="MTLRPLRPEDRDIEFAFVSGLSPETRSNRLLGGTRAITREYIES
                     LVRIDYSRNMALAATTMLGGETLTGVARYVLEKDGRSAEFAIVIADSWQGRGIGKRLL
                     AKLIDIARQRSLKRLYGDILATNRPMLELVRKLGFKLERQEDPTLTRASLDLGG"
     gene            complement(14270..16030)
                     /locus_tag="VIG70_20475"
     CDS             complement(14270..16030)
                     /locus_tag="VIG70_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY8252977.1"
                     /translation="MSAYQAPLKDMRFALNDLAGLGEVATLPGYQDATPDTVDAILEE
                     ASKFASEVLDPINYSGDREGSKFSAGEVRTPKGFQNAYKKFCEGGWNALPFEAEWGGQ
                     GLPRLVSTPVQEMWKSSNMSFSLCPLLTQGAIEALLLRGSEPLKKAYLPSMVAGKWTG
                     TMNLTEPQAGSDLALVKTKAERSGDHYLISGQKIFITYGEHDLAENIVHLVLARTPDA
                     PEGVKGISLFIVPKFVPDRDGKPGKRNAAKCASIEHKLGIHASPTAVMVYDKAVGYVV
                     GEENRGLETMFIMMNAARFAVGLEGVAIAERAFQRALAFAKERVQGRDLVGGGGSVPI
                     IRHPDVRRMLMLMKSQAEAMRALAYTTAAALDFAQRHPDKELRKKHQAFVDLMIPVVK
                     GWCTETGIEVASLGVQVHGGMGFVEETGAAQYLRDARITTIYEGTTGIQAMDLVGRKI
                     AREGGATAKAWLAELKKLDAKLASHPEIRKGLSEGAKAVEDSVDFIVGAKDPRAQFAG
                     AVPFLELMGIVAGGWQMARAALAAEKKVSEDQAFYEAKIATARFYADHVLVQAPGLRN
                     TVVNGAASVMALSEEQFLAA"
     gene            complement(16027..>16822)
                     /locus_tag="VIG70_20480"
     CDS             complement(16027..>16822)
                     /locus_tag="VIG70_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY8252978.1"
                     /translation="AQIAGVKKVLHADAPQLGEFLAENVAALLLQLSKNYSHVLAPAT
                     SNGKNVMPRVAALLDVQQISDISAVEGADTFVRPIYAGNALATVKSKDPIKVITVRTT
                     AFDAVAASGGTAAVENVAAPADSGLSAFVGREVSKSERPELTAAKIIVSGGRGMGSGE
                     NFTKVLEPLADKLGAAMGASRAAVDAGFVPNDWQVGQTGKIVAPDLYVAVGISGAIQH
                     LAGMKDSRVIVAINKDEEAPIFQVADFGVVGDLFQLVPSLVEELKK"
BASE COUNT         2817 a         5787 c         5465 g         2753 t
ORIGIN      
        1 ggggtctaca tctcgcccga cggcaagctg gtcagcgccg cggtcgagct caccaacagc
       61 gtcgcgttca tcgataccgc gagcgatacg gtggtgtttc gcgtcaagat caagggcgac
      121 aatcccgagc acgcggtgtt ctcgcccgac ggccgcttcg tctacgtgag cgccgaggag
      181 gacgacaagc tcgaggtcat cgacgtcgcc aagcgcgcgc tcgtcacgca gctcaaggtt
      241 ggaacgcgcc cgcgcggcat cgccttcacg cccgacagcg ccaaggcgta cgtcgcctgc
      301 gaaagggccg acaccgtcta cgtggtcgac gcgcgccagc acaagatcct gcgcaccatc
      361 aaagtcggcg tgcgctcgaa cggcctcgcg atgcatccgg acggcaagcg cctgtacctg
      421 agcaacggcg gcgccgccaa cgtgatggtg atcgacaccg gcaccgacca ggtcgtcgcc
      481 acgatcccgg tcggccagcg gccctggaac atggcgctga gccgcaacgg cgcgaagctc
      541 tatgtggcga acggccgatc ggacagcgta tcggtcatcg acaccgcaaa gggcgagcgc
      601 atcaaggaca tcccggtcgg caaaacgccc tggggcgtgc acatcctcga ctgagcgtcg
      661 ctcagagcac ggcgaagttg cgcatcatca tgccgtcctc gtgctcgagg ttgtggcagt
      721 ggaacatgta gcgctgccgg ccgcggaacg ccgcctcgtc cggcagcgcg aaatccacgg
      781 ccagccatag cgtttcgttc ggccagacaa ccacggtgtc cttcaccccg aggtcggtcg
      841 gcaaccgccc ctcctccccc gccaatgcac gcgccggtcc gaatgtcccc cggcgccgca
      901 atacgcgaaa gccgaagccg tgcaggtgca tcgggtgcgg catcgagatg gggctgttcc
      961 gcacttccca cacctcgcgc gcgccgcgct cgacgctgaa cgcgatttcg tcggcgcggt
     1021 aaggcgtgtt gtcgatcagg aagcccgcgt gctcatcgaa gtccaggcgc aagcgccgca
     1081 ctgtggcctg cgggggctcc gggagcgtcg acagccgcgc gggaagcggc ggtagctggc
     1141 ctgccccggt tccggccacc tgcaaggtga aaatgccgag ctcggccccg tccggcagcg
     1201 catccgcact gctcgcgcgc tcgccgcaca ccgtctcggc ggcgagcggc gcgtagcgcg
     1261 cgcgcgcggg gtgggcatgc gcgagccgcc cgcctttcgc ctggtggcgc ggatcgaaag
     1321 cgaggctgac cgcgacgagc tcgcggcgcc cggaagcatc gatcgcgaga tcgacccgtt
     1381 cgccgggata aacgaacaca cgatcgacac cgaccggggc cgcaagcagc ccgccatcgg
     1441 ttccgagcaa gtggaacggt acgttcgcat ccgcatcccg gaaagcgagc agcaacccgc
     1501 gtgcattgca cgcgttgagc agctgcagcc gcacccagcc gggcgcgacc gtgcggcgca
     1561 cgtcgagggc tgcgttgacc agcgcgcggt ttccgtacca gccgtgcagg cagtcttcgg
     1621 cgctgggggc atagggacga atcgagccgg ccgccgcgcg gacatcggac atggcgaggg
     1681 ccaggcgatt gtcgggcccg agggcaagcg cggcgtcgag cgcggcgtct tccgcgtcta
     1741 cgacgaccaa cagcccgacc aggcccgagt agacctgctc ggcggtgaag ccatgcgcgt
     1801 gcgggtgaaa ccagtacaac ccgctgcgat tcttcacctc gaagctcaga gccttggccg
     1861 cgccgggcgc gatcgcgtcg aaccctgctc cgtcctgcgc ttcggaaagc gtgagcccat
     1921 ggaagtgcac ggtggtcggc tgcggcagcc ggttctcgat gactgcatcg aaccgctcgc
     1981 cgcgccgcgc gaccagcacg gggtggacga tctggcgccc gccgacgtcc gcggcgtaac
     2041 ggcagcggcc cagaaccggg tcgacgcgct cgacgatcag cctgcgcacc gcgccgatcg
     2101 gcacggcctg cgacgccccg gcggcgggga cccgccgcgc cgcaggcgcg ctgcatgccg
     2161 cgccgatcga tgcggcgccg atcgcaagaa actcccgccg gttcacaagg agagatgttt
     2221 acaccatccg gcgagcttcg cggcgattca ttcccaacgc ttgtcgcccc acaacaggat
     2281 ttgctcaatc gccgccgagg ggcgtggcat agtcgttttc gcgactacca aggctggaac
     2341 tgggagaaac catgacgtct ttgaaaaaac tcttgcacgt gttcgccggc gcgctcctca
     2401 ttggactcac atcgggaaaa gccgtggcgc acgatcgcga cgatgatgac cgcgaccggc
     2461 atcgccacca cggcgaccag cagttccaga tccaggtgct ctctggccgg cccgacacgg
     2521 tggccggcgg cgatgcgctg gtgcgcatct cggccgggaa aaaggacgtg cgcctgtcag
     2581 acgtccgcgt cgagctgaac ggcaggaata tcacgagtgc cttcgtcgcc gacccgggcg
     2641 cgaagacgct gaccggcctg gtcacgggca tgcggatcgg acgcaacgag ctggaagtcg
     2701 acgccaagcg caagggccac ggccgcgccg acgccgacct cgtgctcacg aaccatccca
     2761 tcgagggccc ggtgttctcg ggcccgcacg aggagccctt cttctgccag acgcaccagt
     2821 tcaacatgcc cgcggggctg ggcaccctca cgcccacgca gatcaccgac ccctgccaca
     2881 ttccgatgcg ggtcgactac atctaccgca ccaacgccac gccatccgcg ctcgcgcaat
     2941 ggccggccgg cgcgacggcc tatccggcca acatggcgat gaccgccaag ggcaagccct
     3001 acatcctccg gctcgagacc ggcacggtga accgctcgat ctaccagatc gccatgctgc
     3061 acgatccgat cgcgcagccc gcggcgccga cctggagcaa ccgctcggcg aactggaacg
     3121 gccgcatggt cttcaacttc ggcggcggct gcccgagcgg ctggtaccgc cagggcgcga
     3181 acaccggggg cgtcacggat gacttcatcc tctcgcaggg ctacgctctc gtcagctcct
     3241 cgctgaacgt tgccggcaac aactgcaaca acgtgaccac cgccgagtcg atgatgatgg
     3301 tgaaggagcg cttcatcgag cgctacggcc cgcccgtgca tacgcagggc ttcggctgct
     3361 ccggcggctc gtaccagcag cacgagatca ccgacaacta ccccgggctg ctcgacggca
     3421 tcatccccgg ctgctcgttc ccggaagtga ccttctcgct gctgacgtcg ctcgcagacg
     3481 cctggctgct cgacacctac ttcaagcgca gcgacatcgg cgcgtggacc accgagcaaa
     3541 agcgccaggt cagcggcttc ctgaagtttg agagcatcgc ggccggcgct gcaatcgcta
     3601 accgcatcga ccccgacggc aactgcggcg tcgtgccggt ggagatgcgc tacaacgcgg
     3661 tcaccaaccc gaccggggta cgctgcacga tctacgacca cgcggtcaac atctacggga
     3721 cggatccggc gaccggcttc gcgcgccggc cgctcgacaa cgaaggcatc cagtacggcc
     3781 tgaagctggt caactcgggc gccatcagcg tcgagcagtt cctgagcctg aacgagaaga
     3841 tcggcggctt cgacaaggat gccaacatcg tgccgcagcg cactcaggcc gacctgctgg
     3901 cgacgcgcat ctcctaccgc accggcagga tgaccaacgc cggcgccggg ctcgccgccg
     3961 tgccgatcat cgactaccgt gcgtacgtcg acaacgccac caacggcgaa gtgcatctcg
     4021 cctaccactc gttctcgatg cgcaagcgcc tggagaacga gaaccggcgc tccgacaacc
     4081 aggtaatggt gctggaggac agccgcttcg ggctctacag cagcgcgagc ccgctgctcc
     4141 agcgcatggt cacgacaatg gaccgctgga tcaccgcgat caaggcggac aagcgcagga
     4201 tgtccatggc cgacaagatc gcggccaaca agcccgcgga tctcctcgag ggctgcaata
     4261 cgggcggcac cagtccgacg ttcatgcccc agacgctggt ccgcgaacag gaagccgcca
     4321 ccgcgtgcaa ccagctctac ccgagcaact cgttcccgcg cgaggtcgcc ggggcggaca
     4381 tcgcggccga catcatcaag tgccagagga gagcgccgcg gcaaagcgac tacgcagtga
     4441 ccttcacctc ggcccaatgg gcacgcctgc aggccatttt cccgaccggc gtttgcaatt
     4501 ggtccaggcc aggttacgag caacaggacc tcaagggaac ctggctcgat ttcgattgag
     4561 cccttcttca gtcgcctagt tcggggcggc tgcggaagtg ctcgagcgcc tcggggttgg
     4621 cgagcgcctc gaggtttttc accgctcgcc catgcacgac atcgcgcacc gcgagctcca
     4681 cgatcttgcc gctcttcgtg cgcgggatgt cgccgacctg caggatcttc gcgggaacat
     4741 ggcgcggcgt ggtgttggct ctaacgtgtg agcgaatccg cgcggtcaac gcgtcatcca
     4801 ggctaagccc gtccttcagc ttcacgaaga gcaccacccg gacatcgccc tgccagtcct
     4861 ggccgatcac cagcgactcg gccacctcgt cgaggctctc cacctggcgg tagatctcgg
     4921 cggtgccgat gcgtacgccg cccgggttga gcaccgcgtc ggagcggcca tagatgacga
     4981 tgccgccgtg cgcggtctcc tcgcaccagt cgccatggcg ccagacgccc gggtacttct
     5041 cgaagtaggc ggcgcggtac ttggctccgt cggggtcgtt ccagaaaccg accggcatcg
     5101 acgggaacgg cttggtgcag accagctcgc ccttctcccc tttcaccggc tttccctgtt
     5161 cattgaacac ctcgaccgcc atccccagcc ccttggcctg gatctcgccg cgccagaccg
     5221 ggcccatcgg attgccgaga acgaagcagg acacgatgtc ggtgccgccg gaaatcgagg
     5281 acaggcaaac gtcttccttg acgttggcgt agacgtagtc gaagccttcg ggtgcgagcg
     5341 gcgagccggt ggacaggatg gcgcgcagct tggccaggcg gtgggtttcc ttcggcttca
     5401 ggccggtctt ggcgagggcg tcgatgaact tggccgaggt gccgaaatgc gtcatgccct
     5461 cggcatcggc aagatcgaac atcaagttgc cacggccgac gaacggcgag ccgtcgtaga
     5521 gaaggagcgt ggcgcccgaa gcgagcccgg agacaagcca gttccacatc atccagccga
     5581 gcgtggtgaa gtagaacagg cggtcgcccg gcttcacgtc gctgtgcagg cggtgctcct
     5641 tgagatgctg cagcagcgcc ccgccggtac cgtgcacgat gcacttcggc acgcccgtgg
     5701 tccccgacga gtacaggatg tacaacggat ggtcgaactc gacctgctcg aagcggatct
     5761 cggccgggtc gaacggctcg aggaaatcgt agagcgaggt gccggccgtt accggagtgc
     5821 cgaggtagtc gactacgacg cactcctcga cgctcggcag cttctccaag acctggctcg
     5881 ctttttcctg cgaatcgaac tccttgccgc cgtagaggta gccgtcggcg cagaaaagga
     5941 tcttcggctc gatctggccg aagcggtcga gcacgccctg cacgccgaag tccggcgagc
     6001 agctcgacca caccgcgccg aggctcgccg tggcgaggag cgcggccgcg gcttcgggca
     6061 cgttggggag atagccggcc acgcggtcgc ccttcctcac gcccgcgtcg gcgagcgcct
     6121 gggagatacg cgataccagc tggtaaagat tgaaatgatt gattcttctc ttgatcctgt
     6181 cctcgcccca gaaaacgatc gcgtccgagg catcccgctc gcgcagcagg ttttcggcgt
     6241 aattgagctt cgcttccggg aaccatttcg cccccggcat gcgctccgga ttcaccacgg
     6301 tttggctgcc ctttttgccc tcgaccgcgc agaaatccca cacccggttc cagaactcgg
     6361 cgctcgactc gatcgaccag cggtgcagct cctcgtagct cggcttgccc gcgagcctca
     6421 tgaagcgcgc cagctcactc gccgccgcgc gctcgggcga cggggtccag agcggcctca
     6481 ttgcatcaat gagcggatcc tgtcaggaat gcgcaagggt ttctgctcgc ggtcgaggaa
     6541 caccaccgtg gcggcgccgt ccgcgtaggt ttcgccattg acgttcaact catagaaggt
     6601 cccgacgctc gagccgccgg gcgagccggc gaacaccttc acctccaccg tgccgggata
     6661 gttgatcggg cggcggaagt tgcaggaggc gttcacgatg acgatgctgg tctcgcccca
     6721 cgcctcggcg cgcggcacga gcgactcgaa ccacgcgatg cgcgcctgct ccatgtagcg
     6781 aaagtacacc gtgttgttga cgtgccccat cgcgtccatg tcgccccagc ggacggcgat
     6841 gcgctcggtg tgcaccagct ttttttcatt cataaaacca acaaattata attcgcgcca
     6901 tggcgcgcga atcgatgcag tacgacgtgg tcgtcgtcgg cggcggcccc gcgggactcg
     6961 ccgccgcgat ccggctcaag cagcgcacgc cggatcgctc ggtatgcgtg ctcgagaaag
     7021 gctccgagat cggcgcgcac atcctttcgg gcgcggtcct ggatccgcgc gcgctgtccg
     7081 agctgattcc cgattggaag gagaagggtg cgccgctcaa tacgccggtg agccgcgacc
     7141 ggttcctgtt cctcacgcag aattcgaggt tcgaggtccc ggcgttcgcg cttccggctt
     7201 gcttcaagaa ccacggcaac tatgtcgtga gcctcggcaa cgtctgccgc tggctgggcc
     7261 agcaggccga ggcgctgggc gtggagatct ttcccggatt cgcagccgcc gaggtcctct
     7321 acgaaggcaa cgccgtgaaa ggcgtcgcca ccggggacat gggcgtcagc cgcactggcg
     7381 agaagaccgg cgcctaccag ccggggatgg agttgcacgg caagtacacc ctcttcgccg
     7441 agggctgccg cggccacctc ggcaggcagc tcgaggcgcg attcggcttg cgcgagggcg
     7501 ccgacccgca ggtctacggc atcggcctga aggagctctg ggaagtgaag cccgagcggc
     7561 acgagccggg gctcgtcgtg cacaccgcgg gctggccgct cgacgccgac acctacggcg
     7621 gctcgttcct gtaccacatg gacgagcggc tggtcgcagt cggcttcgtc gtcggcctgg
     7681 ggtacgagaa tccttacctt tcgccgtacg aggagttcca acgcttcaag acgcattccg
     7741 cgatacggtc cttctttgag ggagggaaac ggatttccta tggcgcgcgg gccattgccg
     7801 ccggagggct gcaatccctg ccgaggctcg tgtttcccgg cggcgcgctg atcggcgacg
     7861 aggccggttt tctgaacgca tcgcgcatca agggcagcca ctgcgccatc aagtcggcga
     7921 tgctcgccgc cgatgcggcg cacgacgctc tcgccgccgg gcgcgccggc gacgagctcg
     7981 cggcgtatcc ggaagcgttc cgctcgagct ggctccacga cgagctgcac cgcgcgcgca
     8041 acttcaagcc gtggatgtcg aagggcttgt acaccggcac gctcatggtc ggcatcgacc
     8101 aggtgctgtt ccgcggcaag gcgccctgga ccttgcatca tcaccgtgga gaccacgagc
     8161 aaacccggcc cgctgccgag ttttctccca tttcctatcc gaaacccgat ggcgcgctga
     8221 ccttcgaccg cctctcctcg gtgttcatct cgaacaccaa ccacaacgag gaccagccgg
     8281 tgcatctgac gttgaaagat gcctccgtcc cggtgaaggt gaatctttcc cggtatgcag
     8341 gcccggagca gcgctactgc ccggcggggg tgtacgagtt cgtggatctc gacaccaagc
     8401 cgcggctgca gatcaacgcg cagaactgcg tccactgcaa gacctgcgac atcaaggacc
     8461 cgccgcagaa catcgtctgg gtcgcgccgg agggcggcgg cgggccgaac tacagcaaca
     8521 tgtaactccg cagcgccccg cgtgcaaagt tgcgttgtcg catcgcaacg acgccgcttg
     8581 tacagccatg tttccaatgt ttaccgctgc gccacgaagc ccgcgtgtaa ctccgtcgct
     8641 tgcccgctcc ttgcaggaac cgggcatgaa cgacgaacag caggacttcg cggtccagct
     8701 cctcctcatc gaaagggagg cgagcttcgt cctcgaggac ttgcgtcccg gcgtggcgcg
     8761 tgaccggatt cagcacatcg tcacgattgc gtcgctgctc agggcgcgcc tcgacgtggc
     8821 gtcgagcctc attctcccga gaccgccgat gaaggactga ccgttgcgcg gctagtgcgc
     8881 ggtctgggtc gccgaaggcg gtggccgcaa tgaggcgcgc ggccgtcgag tggccgcttt
     8941 gccgcagcac gctcgccacg aggtagccgt cgccgtcggg aagtccgatg tcgcacagca
     9001 cgatgtgcgg ctcgagctcc ctcgcggttt caattccctg cttgctggtg tgcgcgaccc
     9061 tgacctgata cccgcaaagg tcgagctttc tcgaccggta cgcgcggcct gaaccccagg
     9121 cagagcaggc agcccgggca ggtcttctgc gcttgcggaa ccagactttg agcggtgagt
     9181 tccatcgtga gggcaaatga tggaccgcgg aacgccgcct ggatatcggc gacggcggcg
     9241 ccgatgcgtc gaagtcgcct gaaattggct ggcggcatcg gcgacgggca attcccctca
     9301 ccgaaaaatc aggaccctac gctgcgttca gccttcgctt cagcgtgttc acgtccgcgg
     9361 acagcatggc gtcgacgtct tccggaggca ccggccggct gacatagaag ccctggatct
     9421 ggtcgcagcc gagctcgcgc agcagccgca cctgctcgtc gtcctcgacg ccctcggcga
     9481 cgacgcgcag ccccagggtg tgggcgagcg acacgatcat cgccacgatg ttgcgcggat
     9541 agccggcatc gcgcatgcgc accacgaagc tgcggtcgat cttcagcgtg tccaccggca
     9601 gccgggcgag gtagctgagc gagcagaagc cagtgccgaa gtcgtccacc gacacctcga
     9661 cgcccgcgcg ccgcagcgcc tgcagcttgc gcgtgctctc ctcgatgtcg tcgaccagca
     9721 cgctctcggt gatctcgatg tcgagcaacg gcgccttgcc gtcgaactcg cagatggcgt
     9781 cgagcaccga gtcgacgaag tccttctgcc gcagctgcag cgccgacacg ttgacggagg
     9841 tgcgcggcac ctggaggccg aggccctgcc agcggcggat atcctcgacg gtcttgcgca
     9901 gcgcccagcg cccggcggcg aggatcattc cggtctcctc gagcagcgac acgaagcgcg
     9961 cgggcggcac cgagccgagc tcggggtcgt tccagcggat gagcgcctcc aggcccgcca
    10021 cggccccggt gccgacttcg atcttcggct gatagtgcag cgcgagggtg ccgtcggcga
    10081 gcgcgcggcg caggcggttc tccatgtcca gcgactcggc gacgcgcgcg ttcatctcgg
    10141 gcgcgtagaa gagatagtgg ctgccgctct gcttggcctt gatcagcgcg gtctcggcat
    10201 tcgcgcacag cgcctcggtg gactcgccgt ccgtcgggaa cacggcgatg cccgccttca
    10261 gggacacgcg cagctcgacg ccttcgatca cgatcggctc cgccagcgcc tggtccaagc
    10321 tctcgaggaa cgcatgggcg gtgtcgcccg gacggtcgaa gggcgcgacc gcgatcgcga
    10381 aatgatcggc gccgacacgc gccaccgtgt cctgctcgcg cgccgcctgg cgcagccgcg
    10441 cgccgacctc gcgcagcagg tcgtcgcccg ctttacgccc gaagctctcg ttcaccatgc
    10501 ggaagcgctc gacgtccacg aagatcgccg cggcgaagcg cttctcgcgc cgcgccgcgt
    10561 tcagcgcctg ggtcatgcgg tcggtgaaca ggctccggtt cggcaggtcg gtgagcgagt
    10621 cgtggtacgc gaggtagtcc atgcgcgcct tctgcgcgag atggcgcagg gcgaacgcga
    10681 tgtcgccggc aagctcgtgc agcaggcgca tctcctcctc gtcgaagaaa tcgcgctgcc
    10741 gcgcatagag gagcaggatg cccgcggtct cgccgccggc gagcagcggc agcgcgacga
    10801 acgacccgct ttgcgcgtcg ttccagatga gcgagcgcgt gccgagcttg aggcgctgca
    10861 ggtcgcgctc ggcgctgcct tcgcccgccg acgccacctg gcggatggcg tgcgtcagcg
    10921 ggtcggccag cccgatcgcc gcgagccgga agccgccctt gtccaccgcg atgcggcacg
    10981 cttcgtcgag cagcgcctgg cggtcacccg cccgcgcgat gagtgcattg accgcgctaa
    11041 gcacctcgag ggtgcggttg agccgctcga tgcggcgctg ctgcgcttcc agcgccccgg
    11101 tcttctcgag gagcttgtcg accattacgc gcaggtgctc ctgatcgaga tctccgagga
    11161 tctcggctgg cgcctcgttg cgcgaagcga gcgcggcgtc cacttgccgc aggatctcgg
    11221 cgttctccga gggcttgagc agcacgcgcg acacgccgtg ggcctgcgcg agcgcctgcg
    11281 cgtcctgccc ggcgaagtag gcggtgtaga agatcagcgc cgcggtcgca gtgaccggat
    11341 cggctttcag ccggcgcgcg agctcgtagc cgtccatcat cggcatcagc acgtcggaga
    11401 tcacgagctc gggacgggct tcctgcgcgc gctccagcgc ctcgcggccg tcgatcgcgc
    11461 gcaccacatg gtgcccggcg gtctcgagca gcgcggccag cgcctcgcgg ttggcgcggt
    11521 tgtcgtcggc gaccagaatc gtagccatcg tggaatggac aatcttcgct caaggtcgtg
    11581 cccgcgaatg ccgcgcgctg ccaagtattt cgtagtgaat ccccggaatg acttgtcgcg
    11641 attcggcgac aggacccgga acaggggttg cagtagacat gtatggcgct caagccgaac
    11701 gcgcgcacct gccggatccg cgagcagacc gtcgaagacc tgccgagcgg cctcacgctg
    11761 cactttcagg cctgcggcgg cgacggggcc cgcctggtca tcgccggaaa ggttctgccg
    11821 tgcggcagcc acgagatcgt attcgacacc gaagggcgca ccgtccgcgt cggaccggtg
    11881 caattgaaaa aattgcctct gtagcggccc gcacgacacc gctagaatga gggcatggga
    11941 gagagcgcgg cggggtaccg ccgttttgcc gatttctatc cgttctatct gtcgcagcac
    12001 acagaccgca cctgccgccg gctgcacttc gccggcacca cgctcggcct gctcgcgctg
    12061 ctgcacgcgt tctctacgct gaatttctgg tggctgctcg ccggcctcgc cgccggctac
    12121 gccttcgcct gggtcgggca cttcgttttc gagaagaatc gccctgccac gttcacgcac
    12181 ccgctctaca gcttcatggg ggactgggtg atgtggaagg acatgctgac cggcaggatc
    12241 cggctttgaa cggcaccgcc agcccgttcg gattctggac ctcggctgcg ccgtggctcg
    12301 cgctggtggc gttcggcctc gctgtcgcgc tgctgcgcgc gcgccccgcc gagcgcgcca
    12361 cctatctcaa tacgctctgg ctgttcctgg tcggcatcgg cggcgaagcg gccgcgtcgc
    12421 tcatctgggc gctgaacttc gtggatgccg cgggcaccgt ccacgccatc tcccgcttcg
    12481 tcgcctcgat cgcgcttatc cgcctgtgcg gcttcgctgc gttccggctg ctgcttccgc
    12541 tcgccggcag ccgcccgccg cgcatcgccg aggatctcac gatcgtcgcc gtgtacgtcg
    12601 tctacggctt cgcgcagctg cgcggcgccg gcctcgacct ttccagcatc gtcaccacct
    12661 ccgcgatcct caccgcggtg atcgcgttcg cgatgcagga cacgctcggc aacgtgctcg
    12721 ccggcgtcgc ggtgcaactc gacaactcgg tacgcatcgg cgactggatc cgcgtcgaca
    12781 cggtgagcgg ccgggtgcgc gacatccgct ggcgctccac cctgatcgag acgcgcaact
    12841 gggaaacggt ggtgatcccg aacagcatgc tgatgaaggc gcgcgtcgag atcctcggcc
    12901 agcgcgacgg ggcgccgctg cagtggcggc gcgtcctcga cttcatggtc gacccgggcg
    12961 tgccgccggc gcgcgtaatc gcgatcgtgg aggacgacat gcgcgagctt tcgatcccga
    13021 acgtcgcgcg caccccggcg ccgagctgcg tcctgaaagg cttcgaggaa gggaatctgc
    13081 gctaccagtt gcgctacttt ctgaccgaaa tcaacgagga cgacctcacc gactcgatgg
    13141 ttcgggtgca cctcttcgca tcgctgcagc gcgccggaat ccgcgtcgcc gagccgcagc
    13201 gcacggtgca tgcggtgcag cgcgacgagg cgcacgccga gaccgtgcgg cggcgcgagc
    13261 tcacgcgccg cctgcaggcg ctcgccagcg tgccgccgtt cgccaagctg cccgaggaga
    13321 ccaaggcaga gatcgccgag cggctgcagt acgcgccgtt cgcgcgcggc gacgtcatga
    13381 ccaagcaggg caacaaggcg cactggcttt acatcctcgc ctatggcgag gccgaggtgc
    13441 tgtacgagcc gcccagcggc gcgccgagcg tcatcggcac ggtgcgcgcc ggcgaattct
    13501 tcggcgagat ggccctgatc accggcgacg ctcgcaccgc taccgtggtg gccaagaccg
    13561 acgtcgagtg ctaccgcctc gaccgggcct cgttccagga gttcctgctc gggcgcgccg
    13621 agatctccga tgacatcacc cgcgtcatgg gcggccgccg cggcgacctc gagcgcgtgc
    13681 gccaggcgtt cgccaccacc gtcccggtgg ccgaggccga agtgcgcctc gccaccaaga
    13741 tccgccgctt cttcgggatg cgcacataga cgacttcacc gagtccttcg agcccatcgt
    13801 cgggacctcg gtgacgctgc gcccactacg ccccgaagac cgcgatatcg agttcgcctt
    13861 cgtctccggg ctttcgccgg agacgcgcag caaccgcctc ctcggcggca cccgagccat
    13921 cacccgcgag tacatcgaga gcctggtcag gatcgactac tcgcgcaaca tggcgctcgc
    13981 agcgaccacc atgctcggcg gcgagaccct gactggcgtc gcacgatacg tcctggaaaa
    14041 agacggcagg tcagccgaat ttgcgatcgt gatcgcggac tcgtggcagg gacgcggcat
    14101 cggcaagcgg ctgctcgcca agctcatcga catcgcccgc caacgtagcc tgaagaggct
    14161 ctacggcgac atccttgcga ccaacaggcc gatgctcgag ctggtgcgca agctcggctt
    14221 caagctcgag cgccaggagg atccgacgct gacgcgcgcg tcgctcgact taggcggcta
    14281 ggaactgctc ttcggagagc gccatcacgc tggcagcgcc gttgaccacc gtattgcgga
    14341 gccccggcgc ctgcaccagg acgtggtcgg catagaagcg cgcggtggcg atttttgcct
    14401 cgtagaaggc ctggtcttcc gacactttct tctccgcagc cagggcggcg cgggccatct
    14461 gccacccgcc ggcgacgatg cccatcagct cgaggaacgg cactgcgccg gcaaactgcg
    14521 cgcgcgggtc tttcgcgccg acgatgaaat cgacgctgtc ctcgactgcc tttgcgcctt
    14581 cggacaggcc cttgcggatc tccggatggc tcgcaagctt ggcgtcgagc ttcttcagct
    14641 cggcgagcca tgcctttgcc gtggcgccgc cctcgcgcgc gatcttgcgg ccgacgaggt
    14701 ccatcgcctg gatgccggtg gtgccctcgt agatggtggt gatgcgcgcg tcgcgcaggt
    14761 attgcgccgc gccggtctcc tcgacgaaac ccatgccgcc gtgcacctgg acgccgagcg
    14821 acgcgacttc gatgccggtc tcggtgcacc agcccttcac gacgggaatc atcagatcta
    14881 cgaaggcctg gtgcttcttg cgcaattcct tgtcgggatg tcgctgagcg aagtcgaggg
    14941 cagcggcggt ggtgtaggcg agagcgcgca tcgcctcggc ctgcgacttc atcagcatca
    15001 gcatgcggcg cacgtcgggg tggcggatga tcggcaccga gccgccgccg ccgacgaggt
    15061 cgcggccctg cacgcgctcc ttggcgaacg cgagcgcgcg ctggaacgcg cgctccgcga
    15121 tcgccacgcc ttcgaggccg acggcgaagc gcgcggcgtt catcatgatg aacatggtct
    15181 cgaggcctcg gttctcctca cccaccacgt agccaacagc cttgtcgtag accatcaccg
    15241 cggtcgggct ggcgtggatg ccgagcttgt gctcgatcga ggcgcatttg gctgcgttgc
    15301 gcttgccggg cttgccgtcc ctgtccggga cgaatttcgg cacgatgaag agcgagatgc
    15361 cttttacgcc ttcgggcgcg tcgggggtgc gcgcgagcac caggtgcacg atgttctcgg
    15421 cgaggtcatg ctcgccgtag gtgataaaga tcttctggcc ggagatgagg tagtggtcgc
    15481 ccgatctctc ggcttttgtc ttgaccaggg ccagatcgga accggcctgc ggctccgtga
    15541 ggttcatcgt cccggtccac ttgcccgcga ccatgctcgg gaggtaggcc ttcttgagcg
    15601 gctcggatcc acgcaggagc agcgcctcga tcgcgccctg ggtgagcagc gggcacaaag
    15661 agaagctcat gttcgagctc ttccacatct cctgaaccgg agtcgaaacc agcctgggca
    15721 gtccttggcc gccccactcg gcctcgaacg gcagcgcgtt ccagccgccc tcgcagaatt
    15781 tcttgtaggc gttttggaac cccttgggcg tgcgcacttc cccggcgctg aatttcgagc
    15841 cttctcggtc tcctgaatag ttaattggat caagaacttc agatgcgaac ttcgaggctt
    15901 cctcgaggat tgcatcgacg gtgtccggag tcgcgtcctg gtatccggga agcgttgcca
    15961 cctcccccag tcccgcgaga tcgttgaggg cgaacctcat atccttcagt ggagcctggt
    16021 aggcgctcat ttcttgagct cctcgacgag cgacggcacg agctggaaca ggtcgcccac
    16081 caccccgaag tcggcgacct ggaagatcgg cgcctcctcg tccttgttga tggcgacaat
    16141 gacccggctg tccttcatcc cggccaggtg ctggatcgcc ccggaaatgc cgacggccac
    16201 gtagaggtcc ggggcgacga tcttgccggt ctgtcccacc tgccagtcgt tgggcacgaa
    16261 gccagcgtcc accgccgcgc gcgaggcgcc catcgccgcg ccgagcttgt cggccagggg
    16321 ctcgagcacc ttggtgaagt tctcccccga gcccatgccg cggccgccgg aaacgatgat
    16381 cttcgccgcg gtgagctccg ggcgctcgga tttcgagact tcccggccca cgaaggcgga
    16441 cagcccgctg tcggcgggcg cagcgacgtt ttcgaccgct gcagtcccgc ccgaagcggc
    16501 gactgcgtcg aaagcggtgg tgcgcaccgt gatgaccttg atcgggtcct tcgatttcac
    16561 ggtcgcgagc gcgttgccgg catagatcgg ccgcacgaag gtatcggcgc cctccactgc
    16621 ggagatgtcg gagatctgct ggacgtcgag cagcgcagcg acgcgcggca tgacgttctt
    16681 gccgttggag gtcgcaggcg ccaacacatg ggaataattc ttcgatagct gcagcagcag
    16741 ggccgccacg ttctcggcca ggaactcgcc cagctgaggc gcgtccgcgt gcaggacctt
    16801 cttgacaccc gcaatctgcg cc
//
LOCUS       DATBSZ010000317         5815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U4170
            k141_409386, whole genome shotgun sequence.
ACCESSION   DATBSZ010000317 DATBSZ010000000
VERSION     DATBSZ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959068
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:29:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,102
            CDSs (total)                      :: 4,063
            Genes (coding)                    :: 4,023
            CDSs (with protein)               :: 4,023
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 1 of 40
            Pseudo Genes (incomplete)         :: 39 of 40
            Pseudo Genes (internal stop)      :: 0 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409386"
                     /isolate="SMAG_U4170"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VIG70_20485"
     CDS             complement(<1..486)
                     /locus_tag="VIG70_20485"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEY8252979.1"
                     /translation="MSYLESETEAQEWVRVFVQALQALGWRDGVNLKLHHRWRGAGPE
                     VLQTNAAELAGLELDAVLAGATPAAMALKRATQSVPVVFANVADPVGQGLVAGLAQPG
                     GNMTGFGAFEYSIAGKWVQGLKELVPSARRFSIIVNPETAPFYRNFLPFIEKAAQQTG
                     IV"
     gene            complement(621..710)
                     /locus_tag="VIG70_20490"
     tRNA            complement(621..710)
                     /locus_tag="VIG70_20490"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(674..676),aa:Ser,seq:cga)
     gene            complement(737..2023)
                     /gene="serS"
                     /locus_tag="VIG70_20495"
     CDS             complement(737..2023)
                     /gene="serS"
                     /locus_tag="VIG70_20495"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY8252980.1"
                     /translation="MIDIQLLRKNPQEVARRLATRGPGAFDLNEFERFETTRKELQSA
                     VQAAQASRNRIAKDIGQAKAKGHDVSELMQQGEKLKSLLEESEKQLERLQAAFQDLLA
                     RIPNVPHESVPVGKSAEDNPERRRWGTPRNFSFAAKDHVDIGEGLGGLDFATATKISG
                     SRFVLMRGEVAHLHRALAQFMLDTHTREHGYTEIYTPYIVNSATAYGTGQLPKFEQDL
                     FVVSHGDGKSYLLPTAEVPVTNIVRDEIVEPKNLPLKFVCHSPCFRSEAGSYGKDTRG
                     MIRNHQFDKVELVHIVHPSKSYEALEELTGHAETVLKKLELPYRVVELCTADIGASAA
                     KTYDLEVWLPAQNAYREISSCSNCEAYQARRIQARFRAENGKPELVHTLNGSGLAVGR
                     TLVAILENFQNADGSVTIPTALRPFFGGQEKIARRA"
     gene            complement(2020..3306)
                     /locus_tag="VIG70_20500"
     CDS             complement(2020..3306)
                     /locus_tag="VIG70_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY8252981.1"
                     /translation="MTDLFGKREPAAPLADALRPQSLDEVVGQRHLIGPGKPLRLAFE
                     SGKPHSMILWGPPGSGKTTLARLMAKAFDAEFIALSAVLSGVKDIREAVQAAEHTLAA
                     SGRRTILFVDEVHRFNKAQQDAFLPYVERGLFTFVGATTENPSFEVNSALLSRATVYV
                     LEPLSPTDLAALLSKTAITIDEDATARLIGFADGDARRLLNAVEILLNTGADKVDAAF
                     LEQTLAKNLRRFDKGGEQFYDQISALHKSVRGSDADASLYWMVRMLDGGADPLYVGRR
                     LVRMAVEDIGLADPRALRVALDACETYERLGSPEGELALAEAVLYLAVAAKSNAVYQA
                     YNAARDFIKADGTRPVPLHIRNAPTALMKNLGYGKDYRYAHDEKDAFAAGEDYWPEGM
                     PSLEWYRPTDRGLEAKIRERLEELRRRNAEALNKRR"
     gene            complement(3303..3911)
                     /gene="lolA"
                     /locus_tag="VIG70_20505"
     CDS             complement(3303..3911)
                     /gene="lolA"
                     /locus_tag="VIG70_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEY8252982.1"
                     /translation="MRFALLLVVLFSATAQAAAIERFKTFVRGTQAARADFEQKVYDR
                     NGKLAQESKGNFVFQRPGLFRWIYAKPVDQVIVGDGERVWIHDRDLNQVTVRKLSRAL
                     GSTPAALLAGSADIEKAFELSDAGTKDGLEWLEARPREREAGFERVRMGFDAEGLRAM
                     ELLDHFGQTTMLRFMNLRRNPKVDKSEFRFEPPKGTDVLGEK"
     gene            complement(3920..>5815)
                     /locus_tag="VIG70_20510"
     CDS             complement(3920..>5815)
                     /locus_tag="VIG70_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY8252983.1"
                     /translation="ASAALESLRLHSLRAQLPLAAGGMIGGTVGALTLTLAGFTGATL
                     ILLTLAALGLSLLTGISWLTVAEAAGFALESAFLALRRAWDRRRDEKIGERSREEREI
                     VVKADKKREEEHAPLVIAPPPPEIRKSERAQKEKQAPLFEHLPDTPLPPLKLLDEPSA
                     EADTTVSPETLEYTSRLIEKKLSDFGVSVKVLAAYPGPVITRYEIEPAVGVKGSQIVN
                     LVKDLARALSVVSIRVVETIPGKSCMGLEIPNPHRQTVRLTEILGSETYHGVHSPLAL
                     ALGKDIAGNPVVADLAKMPHLLVAGTTGSGKSVAINAMILSLLYKAEPKNVRFILIDP
                     KMLELSVYQDIPHLLAPVVVDMKQATNALGWCVAEMDRRYKLMNWIAARNLSGYNHKV
                     ADRKIDDPFSPDPGKPQKLQPLPHIVVVIDELADLMLVVGKKVEELITRLAGKARAAG
                     IHLVLATQRPSVDVITGLIKANIPSRIAFQVSSKVDSRTILDQSGAETLLGAGDMLYL
                     PPGTGLPVRVHGAFVADHEVHKVVEHLKSLAKPEYLGEVLEPAVDEEAAALDGVPTGE
                     KDPLYDQAVEIVLRTRRPSISLVQRHLRIGYNRAARLIEDMERAGMVSAMQSNGNREV
                     LVPAKAE"
BASE COUNT          945 a         1893 c         1947 g         1030 t
ORIGIN      
        1 gacaattccg gtttgctgag cagccttctc aatgaagggc aaaaaatttc ggtagaacgg
       61 tgccgtctcc ggattgacaa ttatgctgaa ccgtctggcc gagggaacaa gttccttgag
      121 cccttggacc cactttccgg ctatcgagta ctcgaaagcg ccaaagccgg tcatgttacc
      181 gccgggctgc gccaagccgg cgacaaggcc ctgaccgacg gggtccgcga cattggcaaa
      241 tacgacgggg acagattgcg tggctctttt gagcgccatc gcagctggcg tagctcccgc
      301 caacacggcg tcgagctcca gaccagccag ttcagcagca ttcgtctgta ggacttcagg
      361 accggctcct cgccagcggt gatgcaattt gagattgacg ccgtcgcgcc aaccaagagc
      421 ctggagcgct tgaacaaata ctctcaccca ctcttgtgct tcggtctcgc tctccagata
      481 ggacatgagg acgccaactt ggcgtacgcg ctgaggctgc tgagccgttg cgcccaaagg
      541 ccaaaggccg gtagcgccaa ggagtctgat gaactcccgc cgtctcatcg atcacctcgc
      601 tggtcgtccg gggagggttc tggcggagag agagggattc gaacccccgg taccctcgcg
      661 ggtacaacag atttcgagtc tgccgcaatc gaccactctg ccatctctcc gcggccgaaa
      721 cgaaacgcga attttatcag gcgcgccggg cgatcttctc ttggccgccg aagaaaggac
      781 gcagcgcagt cgggatggtc accgagccgt cggcgttctg aaagttctcc aggatcgcca
      841 ccagcgtgcg gccgaccgcc agccccgagc cgttcagcgt gtgcaccagc tcgggcttgc
      901 cgttctcggc ccggaaccgc gcctgaatgc ggcgggcctg ataggcctcg cagttcgagc
      961 aggaactgat ctcgcggtac gcgttctgcg cgggcagcca gacctcgagg tcgtaggtct
     1021 tggcggcgga ggcgccgatg tccgcggtac agagctcgac cacgcgatag ggaagctcga
     1081 gcttcttcag gaccgtctcg gcgtggccgg taagctcctc cagcgcctcg tacgacttcg
     1141 acggatggac gatatgcacg agctcgacct tgtcgaactg gtggttgcgg atcatgccgc
     1201 gcgtgtcctt cccgtacgag cccgcctccg agcggaagca cggggaatgg cagacgaatt
     1261 tcagcggcag gttcttcggc tcgacgatct cgtcgcgcac gatgttggtg accgggacct
     1321 ccgccgtcgg cagcaggtag gacttgccgt cgccgtgcga cacgacaaaa agatcctgct
     1381 cgaatttcgg cagctggccg gtgccgtagg cggtcgcgct gttcacgatg tagggcgtat
     1441 agatttcggt atagccgtgc tcgcgcgtgt gcgtgtccag catgaactgg gcgagcgcgc
     1501 gatgcagatg cgccacctcg ccgcgcatga ggacgaagcg gctgcccgag atcttggtcg
     1561 cggtggcgaa gtcgagcccg ccaaggcctt cgccgatgtc cacgtgatct ttggcagcaa
     1621 aggaaaaatt cctgggcgtc ccccagcggc gccgctcagg attgtcttcg gcggacttgc
     1681 cgaccggaac ggactcgtgc ggaacgttgg ggatccgcgc gagcaggtcc tggaaggccg
     1741 cctgaagccg ctcgagctgc ttctccgact cctcgagcag cgacttcagc ttctcgccct
     1801 gctgcatgag ctcggacacg tcgtggccct tggccttcgc ctggccgatg tccttggcga
     1861 tgcggttgcg agaggcctgc gccgcctgca ccgccgactg cagctccttg cgggtggttt
     1921 cgaagcgctc gaactcgttg aggtcgaacg cgcccgggcc gcgcgtcgcg agccgcctcg
     1981 ccacctcctg gggattcttg cgcaggagct ggatgtcgat catcgccgtt tgttgagcgc
     2041 ctcggcgttg cgccgccgca gctcttccag cctttcgcga atcttggctt caagaccccg
     2101 gtcggtgggc cgataccact cgagtgaggg catgccctcc ggccaatagt cctcgcccgc
     2161 cgcgaacgca tccttctcgt cgtgcgcgta acgatagtcc ttgccgtaac cgaggttctt
     2221 catcagggcc gtgggcgcgt tccggatgtg cagcggcacg gggcgcgtgc cgtcggcttt
     2281 gatgaaatcg cgcgccgcat tataggcttg gtacacggca ttcgatttcg ccgccaccgc
     2341 gaggtagagg acggcctcgg cgagcgcgag ctcgccttcg gggctgccga ggcgctcgta
     2401 ggtctcgcag gcgtcgagcg cgacgcgcag cgctcgcgga tcggcaaggc cgatgtcctc
     2461 gaccgccatg cgcacgaggc gccggccgac gtatagcggg tcggcgccgc cgtcgagcat
     2521 gcgcaccatc cagtagagcg aggcatcggc atccgagccg cgcacggatt tgtggagcgc
     2581 ggagatctgg tcgtagaact gctcgccgcc cttgtcgaag cgccgcaggt tcttcgccag
     2641 ggtctgctcg aggaaggcgg cgtcgacctt gtcggctccg gtattcagca ggatctcgac
     2701 cgcgttcagc aaccggcgtg catcgccgtc ggcgaagccg atcagccgcg ctgttgcgtc
     2761 ttcatcgatc gtgatggcag ttttcgaaag cagggccgcc aggtccgtcg ggctgagcgg
     2821 ctcgagcaca tacacggtgg cgcgggaaag cagcgcgctg ttcacctcga acgacgggtt
     2881 ctccgtggtc gcgccgacga aggtgaacag gccgcgctcg acgtacggca ggaaggcatc
     2941 ctgctgcgcc ttgttgaaac ggtgcacttc gtcgacgaag agaatcgtgc gccgcccgct
     3001 cgccgccagc gtgtgctcgg ccgcctgcac cgcctcgcgg atgtccttca cgccggacag
     3061 cacggccgaa agcgcgatga actcggcgtc gaaagccttc gccatcaggc gcgcgagggt
     3121 ggtcttgccc gatccgggcg gcccccacag gatcatcgaa tgcggcttgc ccgactcgaa
     3181 ggcgagcctg agcggcttgc ccggcccgat caggtggcgc tggccgacga cctcgtcgag
     3241 cgactgcggg cgcagggcgt cggcgagtgg agctgcgggc tcccgcttgc cgaatagatc
     3301 ggtcactttt cccctaggac gtcggttccc ttgggcggct cgaagcggaa ttcggactta
     3361 tcgactttgg gattacggcg caggttcatg aaacgcagca tggtggtctg accgaagtgg
     3421 tcgagcagct ccatggcgcg caagccctcg gcgtcgaacc ccatgcgcac gcgctcgaag
     3481 cccgcttcgc gctcgcgcgg ccttgcttcc agccactcca ggccgtcctt ggttccggcg
     3541 tcggaaagct cgaacgcctt ctcgatgtcc gcagaacccg cgagcagcgc cgccggcgtg
     3601 gagccgagcg cgcgtgagag cttgcgcacg gtgacctggt tcaggtcgcg gtcgtggatc
     3661 catacgcgct cgccgtcgcc gacgatcacc tgatcgaccg gcttggcgta gatccagcgg
     3721 aacaggcccg ggcgctggaa cacgaagttg cctttcgatt cctgcgccag cttgccgttg
     3781 cggtcgtaga ccttctgctc gaagtcggcg cgcgccgcct gcgtgccgcg cacgaaggtc
     3841 ttgaagcgct cgatggcagc ggcttgcgcc gttgcagaga aaagtacgac gagaaggagc
     3901 gcaaaccgca tgcctgcctc tactccgcct tcgcggggac caggacctcg cggttgccgt
     3961 tggactgcat ggccgacacc atgccggcgc gctccatgtc ctcgatcagg cgcgcggcgc
     4021 ggttgtagcc gatgcgcaag tgccgctgca ccagcgagat cgacggccgg cgcgtgcgaa
     4081 gcacgatctc gactgcctgg tcgtacaggg ggtccttttc tcccgtcggc acgccgtcca
     4141 gcgccgccgc ctcctcgtcc acggcgggct cgagcacctc gcccagatac tcgggcttcg
     4201 ccagcgactt gaggtgctcc acgaccttgt gcacctcgtg gtcggcaacg aaggcgccgt
     4261 gcacgcgcac cggcaggccg gtcccgggcg gcaggtagag catgtcgccc gcgccgagga
     4321 gcgtctcggc gcccgactgg tcaaggatgg tgcgcgagtc gaccttggag gagacctgga
     4381 aggcaatgcg cgaaggaatg ttggccttga tgaggccggt aatcacatcg accgacggcc
     4441 gctgcgtggc gagcacgagg tggatgcctg ccgcgcgcgc cttgcccgcg agccgcgtga
     4501 tcagctcctc gactttcttt ccgacgacca gcatcaggtc ggcgagctcg tcgatcacca
     4561 ccacgatgtg cggcagcggc tgaagcttct gcggcttccc cgggtccggc gagaacggat
     4621 cgtcgatctt gcggtcggcg accttgtggt tgtagccgga gaggttgcgc gcggcgatcc
     4681 agttcatcag cttgtagcgc cggtccatct cggcgacgca ccagccgagc gcgttggtcg
     4741 cctgcttcat gtcgaccacc acgggggcga ggaggtgcgg gatgtcctgg tagaccgaga
     4801 gctcgagcat cttcgggtcg atcaggatga agcgcacgtt cttcggctct gccttgtaga
     4861 gcagcgacag gatcatggcg ttgatcgcga ccgacttgcc cgaaccggta gtgccggcca
     4921 ccagcaggtg cggcatcttc gcgaggtcgg ccaccaccgg gttgccggcg atgtccttgc
     4981 cgagcgcgag cgcgagcggc gagtgcacgc cgtgataggt ctcggagccg aggatctcgg
     5041 tgaggcgcac cgtctggcga tgcggattcg ggatctccag gcccatgcac gacttgcccg
     5101 ggatggtctc gaccacgcgg atcgagacca cggacagggc gcgcgccagg tccttcacca
     5161 ggttgacgat ctggctgcct ttcacgccga cggcgggctc gatctcgtag cgcgtgatga
     5221 ccggtccggg gtacgcggca agcaccttca ccgagacgcc gaaatcggag agcttcttct
     5281 cgatcaggcg cgaggtgtat tccagggtct ccgggctgac cgtggtatcg gcctccgccg
     5341 acggttcatc gagcagcttc aacggcggca gcggcgtgtc gggcagatgc tcgaagagcg
     5401 gcgcctgctt ttccttctgc gcgcgctcgg acttcctgat ctcgggcggc ggcggcgcga
     5461 tcaccagcgg cgcgtgctcc tcctcgcgct tcttatcggc cttgaccacg atctcgcgct
     5521 cttcgcgcga gcgctcgccg atcttctcgt cgcgccgccg gtcccaggcg cgccgcagcg
     5581 cgaggaaggc cgactcgagc gcaaacccgg ccgcctcggc gaccgtgagc caggaaatgc
     5641 cggtgagcag cgacaagccg agcgccgcca gcgtgagcag gatcaaggtt gcgccggtga
     5701 agccggcgag cgtgagggtc agcgcgccga cggtgccgcc gatcatgccg cccgcggcga
     5761 gcggcagctg ggcgcgcagc gaatgcaggc gcagcgactc gagcgccgcg ctcgc
//
